LOCUS       DAOTON010000001        15843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_3677_length_15843_cov_3.318660, whole genome shotgun sequence.
ACCESSION   DAOTON010000001 DAOTON010000000
VERSION     DAOTON010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15843
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3677_length_15843_cov_3.318660"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1801
                     /locus_tag="P5292_00005"
     CDS             <1..1801
                     /locus_tag="P5292_00005"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HRS37534.1"
                     /translation="FLPAGVFVLRLNHALMKAQPGLQLVDNYEVVMHSDEALITQEIL
                     PRLDVKSFDKEVPIMRITYSSEVPQKAADFSNALAQAYVRDYVESKTEAASKTVQFID
                     ERLDRIGKELRASEQRLESFRLRNRIVNTRQETETGLRKLSDLQVQLTNLDMQETGLN
                     QLAEAVGNRKDMNTIGPAFESFQDPLFTEMIKNLKSYQQERRELLTRYTRDHEKVKLV
                     EEKIDDVNSYLREAISNARKNIGLRRNELTTAVEEADHAFDDLPTKEKNMVNLERDFQ
                     LNQKVYQFLLEKRTEASIAEAATISFHRVIQNAPVPASPVFPRRGFLIALAAMTGFLA
                     SLTFLYARRYFRGVLEDKSTVERLSGIPVAGILHEQNDSRAIGRDFSTLAGQLTLTGA
                     LKFPALVCVTSSLREEGKTYTAAGLAAAFTRSGRRVLLVDLDLRSEVVGESPLGISEV
                     IERGLDPAGLWTQQADGYNLLAAGSRAEDPVALMQRPELPGLFEKLKRQFDLVIVDTP
                     ATAYVLDAVPVLKQADLCLYLLRAGQTPVHQVPFAEQLSVTYDLQNVRFILNGMPPVV
                     NYSGDFTGSRFRYGVRLESRFAKLRHYLRAYVS"
     gene            1798..3072
                     /locus_tag="P5292_00010"
     CDS             1798..3072
                     /locus_tag="P5292_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRS37535.1"
                     /translation="MKRLLNIYHNNRQAPVALLDQALVSGANFVTGVLLARFLGLKEY
                     GVFAMAWLVVLFFGSIQQSFIIAPLQTLLPKREQEERASLRTSLFLIQLCMGLLAMLL
                     TWGFCHLSTTLFLDQTPLGALTLVLPITVFTFLMNEYFRKLFFSEGRQTTALILDLIS
                     YGTQILGLLLVASAGELDLRRALIIIASSNSISTAYGLMRSHGLSADFRHFTRHLKEI
                     SSYSGWLIGTSLLQWCSGNFFIITAGGLLGPAPVGAIRMAQNVVGVLNILFLAMENFI
                     PSNAARIYQQQGLRYLYTYLRQVMFLAGAATLAALSLIMVFAREIIGMLYGAGFEQYD
                     DVLFGFSILYVFVFTGLPLRYFIRTIEKNRDIFVSYVLSAGTSLLLAGPIVSSFGIAG
                     VIAGLVIAQVIVQLWFLYSLRPEISNLWKSST"
     gene            3054..4172
                     /locus_tag="P5292_00015"
     CDS             3054..4172
                     /locus_tag="P5292_00015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS37536.1"
                     /translation="MEIIHLILGKANPDRMNGVNRAVHHLATSQAQNGMHVSVWGITP
                     TPDSAYPDGRLYATRLFRSRKSKLGLDPALSKALREVRPGVVFHFHGGFIPEYFHAAR
                     MLTRQGIPYIFTPHGSYNTVALRKSAWMKKLYFPLFERFVLNHARAIHLLGHSEELAM
                     QQLHTKARHHVVPNGQDQSELQHHHRELPRTQSPVFGFCGRIDAYTKGLDTLVSAFAD
                     YVAAGNPGECWIIGDGPDRTKLEQQVRRLHLQDRIVFFGAVYGEEKLNRIANMDVFLH
                     PSRNEGLPTAVLEASGLGVPCIVSEETNVGSVVRRFNAGIVLEKNNVQSLKESLVRFR
                     YESNRFSLSPLKENAKRMVEEEFDWRIIAHRLQALYLS"
     gene            4175..5458
                     /locus_tag="P5292_00020"
     CDS             4175..5458
                     /locus_tag="P5292_00020"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRS37537.1"
                     /translation="MLDDRYIRILTRWLVVILFLKIAGYFTLSENVAVTRVLKIILRT
                     GMTVAIILVYRDLRSRGAFAIREFGNLIVPVLYGAYLFLGFLSLFWSSDPGYSALQLG
                     MIVESFVFAYYFVLVYRLVLHYRPDASLRVSHLLSRSTGMVLLVFLLGVILLPEKFYR
                     LTHGGEEARLGGYLMNPNELGMLSVVGAGMCCLELLKGTKKGWPIFFLLVAITALVLT
                     GSRSSLIGFTLVLLYFVNKSSNRKVKLATVVTMIAAFPLALSTIIFKGGDVEEVMNMT
                     GRIPFWTALLTEGLPKEPLLGFGFMRIAYEDYFQSVHTYAGQMTHNTFIQVVMNLGLV
                     GLALVLIQVAVTIVAFVRNKDEDFRRFFVALFIPIIINSFTEFGIFGETNYGILFYQL
                     LLFFLAVRVSTRPTFKEQVLSRAMQRPRPRYHSVS"
     gene            5592..8237
                     /locus_tag="P5292_00025"
     CDS             5592..8237
                     /locus_tag="P5292_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRS37538.1"
                     /translation="MFHSLDLPVFLQLFTSGDVDLFTPGNRESGLPVAPSQENTSRRR
                     LAILRDDAETSAVSTWADFILHLHGSSRSLNGYTATTFCCVNNPDGSIRWMYPEGTRH
                     PNFLRLYNSGSLKAQAYRAIVRGAFFLGQGKRVADRRITIYARHETHLERILSALSVD
                     GYSIFTGTVGENRKCVVEVHRSGRRTHFVKIPLNRNSAQLLENENRTLAYLGRAGFRS
                     LQIPQSACHSSGAIFLSDVKPARVKRSRRFSSLHAKALCELYQRYLTVDQLGESDFFE
                     RVMQNLYLLRQRSSRLQQRGMQPLFRKLEQLFRSIDPTQRVHLAASHGDFTPWNTFVS
                     EERVHAYDWELSRRDAPLLSDLFHYVIQGEVMQHRSGYGKIREELERALSLPEVADLC
                     QKFRIDPMLHYRLYLLSNVSYYLNLYVTQEQLHPQARWVMDAWEQAITELLPADVARS
                     RREEILEQFFERYKQAPYAALKFIHGSVEAMGPFTDLDLVVTPEFRDTVIEDARRHPS
                     VFKSRVHRKSFMTTLELYFRDQSYLSIDLLTDFHRKDVAYLDPTRLLRSATPGRSHVR
                     MPDERFGFEYTWLFNLLNGSSLPKRYRTYYQSLTAEERERILSHINSSFGFEAVSLEE
                     FYDYSAYRRQQVVRKLKKSSTNSIVSRWIRSVSYTADTVRSLFERRGMMITLTGVDGA
                     GKSTIIDELKQLLSEKFRRKVVVLRHRPSLLPILSAWKHGKHHAEAIAVARGPRGGSN
                     RGKLSSLARFLYYYSDYLLGQFVVYARYQLRGYTVLYDRYYFDFIVDARRSNISLSEG
                     FTRSLYRLVRKPDLNILLYAPVDDILRRKQELDADTIQELTDGYLRLFDRLSGSSTRS
                     VYLPLENTDKERTLRVIESAIVKAA"
     gene            8241..8966
                     /locus_tag="P5292_00030"
     CDS             8241..8966
                     /locus_tag="P5292_00030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRS37539.1"
                     /translation="MKILLEQIIRLRNPHFRFDESVTSRLLLAFAWSYGRSYLRGLRL
                     LLLAKMPRHVLLGRGVGFFNSPNIRLGRWVKLGDHVQLSAAGTGELVIGDHSGIGAFS
                     RVVIATSFNQLGSHIRIGKNVGIGEFAYLGGGGGLEIGDDCIVGQYFSCHPENHITVD
                     PNRPIRMQGVTRKGIRVGKDCWIGSKVTILDGVEIGDHCVIAAGAVVTRSMPSGSVIA
                     GVPARVIRSRSADEPVTYQHRAS"
     gene            8983..11298
                     /locus_tag="P5292_00035"
     CDS             8983..11298
                     /locus_tag="P5292_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS37540.1"
                     /translation="MKTILLTAYAINPYKGSEDGMGWNFVLQAARHQRVIAVTRRNNR
                     PHIERYLSEHTIAQVNNITFLYFDLPAWTRFWKKGPTLSLIYFYMWQISLPFFLMRYR
                     KQVDIVHNLNFHNDWTPTFLWLLRKPMVWGPVGHHPLIPKQFILPVYGRKAWLADRAR
                     WAMKIIFWKLDPFLRIATRKADLILTMNSEARARLGFSAEKEFRCSSVASEDVAWTRR
                     TTKNGLKVLSVGRFVALKGFDVTLRSFAKFYHALPVEAREHVSLTLVGDGPERSMLER
                     MTDELQIREVTKFIPWIQRAELADLYRSSDVFLFPSHEGAGMVVSEAMSYGLPVVCFR
                     NSGPGEFVSLASGFRVPYDAYDACIDRFAAHLRELHDDSTLWARMSEYARMSFEERFA
                     WSNKGEVLRNAYGRLERQQRILAVQLMNDFSGSPQVLANAIRGFVREGRKVELYCSSA
                     TREGFLSGLPVKTHAFWYRFYPNKWKRLLALTTSQAHLFLKILIKARKNDIVYINTLL
                     PFGAAIAARLRGARVITHLHETSIQPASLKKFLRSVVEHTASRVVHVSYFLREQESFT
                     KPEQLVVYNATSEDFVRMGALHRYEAGNQHPFTVLMLCSLKAYKGVFEYAELARQLPD
                     LRFELVLNASESEIASFFKDRSLPANLQVHPRQSNVHPFYQRAQLVLNLSHPDQWVET
                     FGMTILESMSYGIPEIVPDIGGPTELVDHGVNGFRINVGELDRIAATIRFLEQNRGEC
                     IRLSAQARIKAGRFTIGKMQSELGKAVTWSN"
     gene            complement(11318..12355)
                     /locus_tag="P5292_00040"
     CDS             complement(11318..12355)
                     /locus_tag="P5292_00040"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRS37541.1"
                     /translation="MVKTDSFSRNNFDLLRILAASQVMVAHSFHHLGLDTESAWFQWL
                     DRFPGVPVFFIISGFLVSASLERSKSPADYFTNRFLRIFPALWCCLGISVLAAKCFGN
                     VDFSRWETLPWLLGQISFVQFFNPEFLRGYGTGVLNGSLWTITVELQFYLLLPLLYAV
                     LLRKSKNDQVLGALLVLFFLLSALLWTLDIPRQTEKWLQVTFLPHFSLFLLGIWIRRR
                     ALHESPLLKGKALIWLIVYASLVSAPVPDVFRLPLSRLVLGLLTISSAYTLPGLADNI
                     LQRQDLSYGVYIYHMVAVNAIVELQQTRTLSDFWMVAAATYSLAAMSWWLVEKPYLRK
                     KTGTLKVPVVT"
     gene            12604..15288
                     /locus_tag="P5292_00045"
     CDS             12604..15288
                     /locus_tag="P5292_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37542.1"
                     /translation="MFRRSNFLAAAIVVLLFLCSGHPASALTYYSRTSGGLWTNPTTW
                     STVTYGNATNTGTFPGAGDNVFIGDGYTIYFNTNGGCNNITIGQGASGALEFYNLAIY
                     WMTVTGDITINAGGRFAYGYNSSRAHILYIEGDITNNGTVDLYLDANDNVALVAQGNT
                     NSVISGTGSWDLYTVLMSKITSRSNYLEVQSSAFETAMRGLTLNYGTYVHNNSGTYTF
                     GSGSDYTIPPNVAIDGRSGTLNFSSGATNLNLQGALIVSGGTVYIASSTGTGGIRTDQ
                     GVAGVVPYLQVSAGQLVVYGSISFRTAPSSSFAEPFSYNQSGGTVLVNSGSTGVTSEV
                     FYVTDQATSSFVMSGGTLNLQRPNRTGSSVVDMFICGNSGTVNSTGGIVQFGNASTPV
                     GSTFNFRPFPNATYPAFRITGPSGSGNSLRTSNASTSNFRLLAMVIDAGTTFDIRSIA
                     GTAGNSKRMSLRGTFDGNSAWYNDGTFNPRSGEVAFEGTAAQTLDGSTDTWFYNMTMN
                     NAAGLTMNARAFVDNSLNMTNGIIYTDYTNLLSVSSSGNTTIGTSSSYIIGPFNQIYA
                     QTSAATGYFPLGKGANWRPMTLTPSHTTSDSVNYVGEMIESPASGLPYTLPGSLSAVS
                     ARRYWLVTNTNTGIFNNATARLYYGTDDGVTDRLSLRVGQAISPNWVNRGGTGTANGT
                     GNITSSSFNAWGAIYTLANATGGSNALPIELVSFVAESREKDVELRWTTATEINNDFF
                     TVERSRDGLHFEAVAQTAGAGNTTTLQHYKAYDLAPLNGTSFYRLRQTDFDGRSTTSD
                     VVTVYRKGNTDFSVYPNPIDADDDVMIRLPHDTNRNLLIQIKRSDGSLVFERTYPVWN
                     EQTIRLSGLPLEASGLYVLSVFTDDGGAYQQKLMANIR"
     gene            15323..15685
                     /locus_tag="P5292_00050"
     CDS             15323..15685
                     /locus_tag="P5292_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37543.1"
                     /translation="MKTPILLSVFAFSALTSFAKGTGKQEQIASNFEQEYRVNSMEAI
                     RNMGDVIFDNPELGSQRHPGKFQPGFPEALRPYDTNKDGKLTTNEIKPVIDRYLSSQT
                     EFSSREMVRFIDYFFSQF"
     gene            15694..>15843
                     /locus_tag="P5292_00055"
     CDS             15694..>15843
                     /locus_tag="P5292_00055"
                     /inference="COORDINATES: protein motif:HMM:NF020874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HRS37544.1"
                     /translation="MKQEREQLAIIGCAGVPARYGGFETLADHLVRELRRDYRITVYC
                     SGPMYR"
BASE COUNT         3379 a         4357 c         4312 g         3795 t
ORIGIN      
        1 gtttcttccg gccggcgtat ttgtcctgcg tttgaatcat gcgctgatga aagcgcagcc
       61 gggactgcaa ctggtcgaca actacgaagt cgtgatgcac tcggatgagg cactgatcac
      121 acaggagatc cttccgcgat tggatgtgaa gtccttcgat aaggaagttc cgatcatgcg
      181 cattacctat tcttcggaag ttccgcagaa ggccgctgat ttttccaacg cactggccca
      241 ggcttatgtg cgcgattacg tagagagcaa aacggaagcc gcttcgaaaa cggtgcagtt
      301 catcgacgag cgactggacc gtatcggaaa ggaacttcga gcttccgagc agcgccttga
      361 atcgtttcgc ctgcgcaacc ggatcgtcaa tacccggcag gagaccgaaa ccggcttgcg
      421 gaagctctcg gatctccaag tgcagcttac gaacctcgac atgcaggaaa ccggcctgaa
      481 ccaactggcg gaagccgttg gcaaccggaa ggacatgaac acgatcggac cggccttcga
      541 gtcattccag gacccgttgt ttaccgagat gatcaagaac ctgaagagct atcagcagga
      601 gcggagagaa ttgcttacgc gctatacgcg cgaccatgaa aaagtgaagc tggtagaaga
      661 gaagatcgac gacgtcaaca gttacctgcg cgaagcgatc tccaatgccc ggaagaacat
      721 cggcctgcgc cggaacgaac tcaccacggc ggtcgaagag gccgatcatg cgttcgacga
      781 tcttccgacc aaagaaaaga acatggtgaa cctggaacgc gatttccagc tcaaccagaa
      841 agtatatcaa tttctcctcg agaagcggac cgaagcgtcg atcgccgaag cggccacgat
      901 ttcctttcac cgggtcatcc agaacgctcc tgttccggca tctccggtgt ttcctcgtcg
      961 cggattcctg attgcccttg cggccatgac gggattcctt gcttcgctta ccttcctcta
     1021 tgcgcgacgg tatttccgcg gcgtgttgga ggataagtcg accgtagaac gcctgagcgg
     1081 cattccggtt gcgggcatct tgcacgagca aaacgatagc cgggcaattg gccgcgactt
     1141 cagcacgctg gcaggtcagt tgaccctcac gggtgcgttg aaattccctg cgctggtatg
     1201 cgtcacttct tcgctacggg aggaaggaaa gacttatacg gccgctggtc ttgccgcggc
     1261 gttcacccga tccggtcgtc gcgtgttgct ggttgacctt gatctccgca gcgaagtcgt
     1321 gggtgagtct ccgctcggga tatccgaagt catcgagcgc ggacttgatc ccgccggact
     1381 atggacccaa caggctgacg gttacaacct gcttgctgcc ggtagcagag cagaagatcc
     1441 ggtcgccctg atgcagcgcc ctgaattgcc cggtttattt gaaaagctga aaaggcagtt
     1501 tgacctcgtg atcgtcgata cgccggcaac tgcttatgtc ctggatgcgg tcccggtgct
     1561 caagcaagcc gatctctgtc tgtatctgtt gcgtgcaggc caaacgccgg tccatcaggt
     1621 tccctttgcc gaacaattat ccgtcaccta cgatctgcag aacgtccggt tcatcctgaa
     1681 cggcatgccg ccggtggtca attacagcgg tgactttacc ggaagtcgct tccgctacgg
     1741 tgttcgcctg gagagtcggt ttgccaaact tcgtcattac ctgcgtgcct acgtttcatg
     1801 aaacgactgc tgaatatcta tcataacaat cgccaggcac cggttgcctt gctggaccag
     1861 gctttggtca gcggggccaa cttcgtgacg ggcgtgctgc ttgcgcgatt cctcggcttg
     1921 aaggaatacg gagtcttcgc aatggcctgg ctggtagtct tgttcttcgg aagtatccag
     1981 cagtcattca tcatcgctcc gttgcagaca ctgcttccca agcgagagca agaggaacgt
     2041 gcaagcctgc ggacgtcctt gttcctgatc cagttgtgca tgggcttgtt ggcgatgctg
     2101 ctgacctggg gtttctgcca tctgtcgacg accttgtttc tcgatcagac tccgctcgga
     2161 gcgctcacgc tggtattgcc gatcaccgtg tttacctttc tgatgaacga gtatttccga
     2221 aaactgttct tttcggaagg aaggcagacg accgcgctta tcctcgacct gatctcctac
     2281 ggtacgcaga tcctgggact cttgttggtt gcttcggcgg gagaattgga ccttcgtcgc
     2341 gcgctcatca tcatcgccag ttccaatagc atctcgacgg catacggatt gatgcgttcg
     2401 cacggactat cggcggattt tcgtcatttc acccggcatc tgaaagagat cagttcctat
     2461 tccggctggc tgatcggcac ttcgttgttg caatggtgtt cggggaattt cttcatcatc
     2521 acggcgggcg gcttgttagg accggctccg gtaggagcga tccggatggc gcagaacgtg
     2581 gtcggagtgt tgaacatcct gtttctggcc atggagaatt tcataccgtc gaacgcggcg
     2641 cggatctatc agcagcaagg acttcgttac ctctatacct acttgcggca ggtgatgttc
     2701 ctggccggag cggctaccct ggcggctttg agcctgatca tggttttcgc ccgggagatc
     2761 atcggcatgt tgtatggagc gggattcgag cagtatgatg acgtcttgtt cgggttctcg
     2821 attttatacg tattcgtctt caccggtctg ccgttgcgct acttcatccg gaccattgag
     2881 aagaatcggg acatctttgt ttcgtacgtc ctcagtgccg ggacgagtct gttgcttgca
     2941 ggcccgatcg tcagctcctt cggtattgcc ggtgtcattg ccggtctggt catcgcgcag
     3001 gtgatcgttc aactctggtt cctgtattcc cttcgtcctg aaatttcaaa cttatggaaa
     3061 tcatccactt gatactcggt aaagccaatc ccgatcgcat gaacggtgtc aaccgcgctg
     3121 ttcatcatct cgccacaagt caggcgcaga acggaatgca cgtgagtgta tggggtatca
     3181 ccccgacacc tgattccgct tatccggacg gccggctgta tgcaacccgg ctctttcgct
     3241 cgcgaaagag caagttagga ttggatcctg cgctttcaaa agcgttgcgg gaagtccgcc
     3301 ccggagtggt cttccatttc cacggcggct ttattccaga gtatttccat gcagcgcgga
     3361 tgctgacacg gcagggaata ccctacatct tcacgccgca cggcagctac aacaccgtcg
     3421 cgctgcgaaa gagtgcctgg atgaagaaac tctacttccc cttgtttgag cgattcgtgc
     3481 tgaaccatgc ccgcgccatt catctgctgg gccacagcga agagcttgcc atgcagcagt
     3541 tgcataccaa agcgcggcac cacgtcgtcc ccaatgggca agaccaatcg gagctacaac
     3601 atcatcaccg agaattgccg cgaacccaat ctcccgtctt cgggttctgc ggtcgcatcg
     3661 acgcgtatac caaaggcctc gatacgcttg tatccgcctt tgctgactat gtcgcggccg
     3721 gtaacccggg tgaatgctgg atcatcggcg acggtcctga tcgtaccaaa ctcgaacagc
     3781 aggtgcgtcg cctccatttg caggaccgga tcgtcttctt tggtgccgtg tatggcgaag
     3841 agaaactgaa ccggatcgcg aacatggacg tatttctcca tccttcgcgc aacgagggat
     3901 taccaactgc ggtactggaa gccagtggcc tgggcgtccc ttgcattgtg agcgaagaaa
     3961 cgaacgtcgg ttcagtggtt cgccgattca atgccggtat tgttttggaa aagaacaacg
     4021 tacaaagcct gaaagaatca ctggtgcgat tccgatatga atcgaaccgt ttttcacttt
     4081 ctccgttgaa ggagaatgcc aagcgcatgg tagaggagga gtttgactgg cggatcatcg
     4141 cccatcgcct ccaggctctc tacctgtcct gaacatgctg gacgaccgtt acatccgaat
     4201 cctgacccgt tggctggtcg tgatcctctt cctgaagatc gcaggctact ttacgctttc
     4261 ggaaaatgtc gcggttaccc gggtcttgaa aatcatctta cgaaccggca tgaccgtggc
     4321 tattatcctg gtataccggg atctgcgcag ccggggtgcg ttcgcgatac gggaattcgg
     4381 aaacctcatc gttccggtgc tttacggcgc ttaccttttt cttggattcc tttcgctgtt
     4441 ctggagttcc gaccctggat attcggcact gcaactggga atgattgtag agagtttcgt
     4501 cttcgcgtat tacttcgtcc tggtctatcg gctggtgttg cactaccgtc ccgatgcgtc
     4561 gctgcgtgtg agtcatttgc tttctcggtc caccgggatg gtactcctgg tctttctgct
     4621 gggcgtcatt cttcttccgg aaaaattcta tcggttgacc catggtggcg aggaggcgcg
     4681 attgggtggt tatctgatga atcccaatga actcggcatg ctaagcgtgg tcggtgccgg
     4741 catgtgctgc ctggagctct tgaagggaac gaaaaaaggg tggccgatct ttttcctgtt
     4801 agtggcgatc acggcgcttg tcctaacggg ttcgcgttcc tcactgatcg gattcacctt
     4861 ggtattgctt tatttcgtga acaagagcag taaccgcaaa gtgaagcttg cgaccgtggt
     4921 gaccatgatt gccgcttttc cgctggcgtt atccaccatc atcttcaagg gaggcgatgt
     4981 ggaggaagtg atgaacatga ccggtcggat tccgttctgg acagccttgc tgacagaagg
     5041 cttacccaag gaacccttac tgggtttcgg cttcatgcgt atcgcgtatg aggattattt
     5101 ccaaagtgtc cacacctatg ccggccagat gacgcacaac acctttatcc aggtggtcat
     5161 gaacctcggc ctggtagggc tcgcgctggt gttgatccag gtcgcggtga cgatcgttgc
     5221 ctttgttcgg aacaaggacg aggactttcg acgattcttc gtggctttgt tcatcccgat
     5281 catcatcaat tccttcacgg agttcggcat cttcggcgag acgaactacg gcattctgtt
     5341 ttaccagttg ctgctctttt ttctcgcggt gcgcgtctcg acgcgaccga ccttcaaaga
     5401 gcaagtgctg agcagggcaa tgcagcgacc gcgaccgcga tatcattcgg tttcctgatt
     5461 ttggaatacc gttccttttt ttgaacgatc cggggccttg aggccctttt tttattgaaa
     5521 ttcttgctga attcgggggt tctctttgat ggcatgattt gggcctcgtg cgcgcgctca
     5581 aatagcccaa tatgtttcat tcccttgatc ttcctgtttt cctgcagctc ttcacgtccg
     5641 gcgatgtgga cctgttcact cccggcaatc gcgaatcggg cttgcccgtc gcgccatcgc
     5701 aggagaacac ttcccgtcgt cgcctcgcca tcctgcgtga tgacgcggaa acgtccgctg
     5761 tcagcacctg ggctgatttc attctgcatc ttcacggttc gtcccgttca ctgaacggct
     5821 atacggccac cacgttttgt tgcgtgaaca atcctgacgg ttccatccgc tggatgtatc
     5881 cggagggcac gcggcatccc aatttcttac gtttatataa ttccggttcg ctgaaggcgc
     5941 aggcttatcg cgcgatcgtt cgcggcgctt ttttccttgg acaaggaaag cgcgtcgcgg
     6001 atcgccggat cacgatttac gcgagacacg aaacacacct tgaacgcatc ttgtcggcgc
     6061 tttcggtgga tgggtattcc atcttcaccg gaacagtggg agagaacaga aagtgcgtgg
     6121 tggaggttca ccgatccggt cggaggaccc actttgtgaa gatcccgctg aaccgtaatt
     6181 cagctcagtt gctggagaat gagaaccgga cgttggccta cctgggccgc gccggttttc
     6241 gttccctgca gatcccgcag agcgcctgcc attccagcgg cgcgatcttt ctcagtgatg
     6301 tcaaaccggc ccgcgtgaag cgcagccgtc gattctcttc gcttcatgcg aaagcattgt
     6361 gcgaattgta ccagcgctac ctgaccgtcg atcaactcgg tgaatccgac ttctttgaac
     6421 gggtcatgca gaacctgtat ctgctgcggc agcgatcctc gcgtttgcag caacgcggca
     6481 tgcagccgtt gttccggaag ttggagcagc tcttccgcag catcgatccc acacagcgtg
     6541 ttcacctggc ggcgagccac ggtgatttca cgccctggaa cacgtttgtt tcggaggagc
     6601 gcgtgcatgc gtacgactgg gagttgtcgc gtcgcgatgc accgttgctt tccgacctgt
     6661 ttcactatgt catccagggc gaagtgatgc agcaccgcag cggctatggt aagatccggg
     6721 aagaactgga gcgcgctctc tcgttaccgg aggtcgccga tctttgtcag aagttccgga
     6781 tcgatccgat gctgcattac cggctgtatc tcctgtccaa tgtaagctac tacctcaacc
     6841 tgtatgtgac ccaggagcaa ctgcacccgc aggcgcgctg ggtcatggat gcctgggagc
     6901 aagccatcac cgagctgttg cctgccgatg ttgcgcgcag ccgtcgggaa gagatcctgg
     6961 aacagttctt cgaacgctac aagcaggcac cgtacgccgc gctcaaattc atccacggct
     7021 cggtggaagc catggggccg ttcaccgatc tggatctggt cgttacgccg gagttccgcg
     7081 atacggtgat cgaagacgcg cgccggcatc cgtcggtgtt caaatcgcgt gtacaccgga
     7141 aatcgttcat gacgaccctc gagctgtatt tccgggacca gtcgtacctc agcatcgacc
     7201 tgctgaccga cttccaccgc aaggacgttg cctatcttga tccgacccgt ttgttgcgta
     7261 gtgctacacc cgggcgttcc catgtccgga tgccggatga gcgttttggg ttcgagtata
     7321 cctggctctt caacctgttg aacggaagca gtctgccaaa gcgttatcgt acctattatc
     7381 agtcgttgac ggccgaggag cgcgagcgta tcctgtcgca tatcaattct tcttttggtt
     7441 tcgaagcagt ttcgctggag gaattctatg actattccgc ctaccgtcgt caacaggttg
     7501 tccggaaact gaagaagtct tcgaccaatt ccattgtttc ccgctggatc cgttcggtat
     7561 cctataccgc ggataccgtt cgatccctct tcgagcgccg cgggatgatg atcaccctga
     7621 ccggtgtaga tggcgcgggc aaatccacga tcatcgacga attgaagcaa ttgttgagtg
     7681 agaagtttcg acggaaggtc gtggtgttgc gtcatcgtcc ttcgctgctg cccatcctga
     7741 gcgcctggaa acatggtaag caccatgcgg aggcgatcgc ggtagcgcgc ggtccgcgtg
     7801 gcggcagcaa ccggggtaag ctctcttcac tcgcgcgatt cctctactac tattccgatt
     7861 atctcctggg tcagtttgtg gtctatgcgc gctatcagtt gcgcgggtac accgtgttgt
     7921 acgatcgcta ttatttcgac ttcatcgttg atgcgcgtcg ttcgaatatc agcctgagcg
     7981 aaggcttcac ccgttcattg taccgattgg tgcgtaagcc ggacctcaac atcctgttat
     8041 atgcgccggt ggacgatatc ctccgccgca agcaggaact ggatgcagat acgattcagg
     8101 agctgacgga cggttacctc cgattgtttg atcggttgtc aggctcttcg acgcgatcgg
     8161 tttacctgcc gctcgagaat accgataagg aacgcacgct gcgggtcatc gaaagcgcga
     8221 tcgttaaagc tgcctgatcc atgaagatcc tgttggagca aatcatccgg cttcgcaatc
     8281 cgcatttccg gttcgacgaa tcggtgacca gccgcctgtt gttagccttc gcctggtcgt
     8341 atggccgcag ctacctgcgt ggtctgcgtc tgttgctgtt ggcaaaaatg ccgcggcatg
     8401 tactgcttgg acgcggtgtc gggtttttca attcgccgaa catccgactt ggacgttggg
     8461 taaagcttgg cgaccatgtt caactgagcg cagccggcac cggggaactg gtcatcggcg
     8521 accatagcgg catcggagcc ttcagtcggg tggtgatcgc cacctcgttc aatcaactcg
     8581 gatcacacat ccggatcggt aagaatgtgg gcatcggtga attcgcgtac ctcggcggcg
     8641 gcggtgggtt ggagatcggc gacgattgca tcgtcggaca gtatttcagt tgtcacccgg
     8701 aaaatcatat caccgttgac cccaatcggc cgattcgcat gcaaggcgtt acacgcaaag
     8761 gcattcgcgt cggcaaggat tgctggatcg gctccaaggt caccatactg gacggtgtag
     8821 agatcggtga ccattgtgtt atcgccgcag gcgcggtggt tacgcgttcc atgccgtccg
     8881 gttccgtcat cgccggtgtt cctgcacgtg tcattcgttc acgcagcgcg gacgagccgg
     8941 ttacttatca acatcgcgca tcctgattca ttattaccgc ccatgaaaac gattctcctc
     9001 actgcctatg ccatcaaccc gtacaagggt tcagaagacg gcatgggctg gaattttgta
     9061 ttgcaggcag cgcgtcacca gcgcgtgatc gccgttacgc gtcggaacaa ccgcccgcac
     9121 atcgagcgat acttgtcgga gcataccata gcacaagtca ataacattac cttcctgtat
     9181 ttcgaccttc ctgcatggac ccgtttttgg aagaaaggac caacgctttc actgatctac
     9241 ttctatatgt ggcagatcag tcttcctttc ttcctgatgc gctaccggaa gcaggtggat
     9301 atcgttcaca acctcaactt tcataacgac tggacgccca ccttcctttg gctgctgcga
     9361 aaacccatgg tgtggggacc ggtgggccat catccgttga tccccaaaca gttcatactt
     9421 cccgtctatg gtcggaaagc atggttggcc gaccgtgccc ggtgggctat gaaaatcatc
     9481 ttctggaagc tcgatccttt tctgcgcatt gccacacgca aggccgacct gatcctgacg
     9541 atgaacagcg aagcgcgcgc tcgtctgggt ttctccgcgg agaaggagtt tcgctgttcc
     9601 tcggtcgctt cggaagatgt agcctggacg cgccggacaa ccaagaacgg cttgaaggtc
     9661 ttatcagtcg gcagattcgt agcgttaaag ggattcgacg tcaccctccg ttcgttcgca
     9721 aagttctatc atgcattacc ggttgaagcg cgagagcatg tcagccttac gctggttgga
     9781 gacggtccgg agcgttcgat gcttgaacgc atgaccgacg aattgcagat acgcgaagtc
     9841 acgaagttta ttccgtggat ccagcgcgcc gaactggcgg acctgtaccg atcctcggat
     9901 gtgttcttat tcccctcaca cgaaggagca ggcatggtcg tatccgaagc gatgagctac
     9961 ggcttaccgg tagtatgttt ccgtaacagc gggccggggg agttcgtttc gctcgccagc
    10021 ggctttcgtg ttccgtacga cgcgtacgat gcatgcattg atcgcttcgc ggcgcacctc
    10081 cgggaattgc acgatgactc gacgctgtgg gcgcgcatgt cggagtatgc ccgtatgtca
    10141 ttcgaagaac gcttcgcatg gtcgaacaag ggagaagtgc tgagaaacgc ctatggccga
    10201 ctggaacggc agcaacggat ccttgccgtt cagctgatga acgacttcag cggtagtccg
    10261 caggttttgg ccaatgcaat ccgcggattt gtcagggaag gccggaaggt cgaactgtat
    10321 tgttcttccg ctacccggga aggatttctt tccggcttgc ccgtgaagac acatgcgttc
    10381 tggtaccgtt tctatcccaa taaatggaag cgactgctgg cgctgactac cagccaggcg
    10441 catttgtttc tgaagatcct tatcaaggca cgaaagaacg atatcgttta catcaatacg
    10501 ctgctgccat ttggcgccgc tatcgctgct aggctccgtg gagcccgtgt gatcacgcat
    10561 ttgcacgaaa ccagcataca gccggcttcg ttgaagaaat tcctgcgttc tgtcgtggag
    10621 catacggcca gccgggtcgt gcatgtgtca tatttcctgc gcgaacagga atcgttcacg
    10681 aagccggaac aattggttgt ctataacgcg accagcgaag attttgtccg tatgggtgcg
    10741 ttgcatcgtt atgaggccgg taatcaacat ccgttcacgg tcctgatgtt gtgttccctg
    10801 aaagcctaca aaggtgtatt cgagtatgcg gagcttgcac gccaactccc ggatctgcga
    10861 tttgagctgg tgctcaacgc gagcgaatca gaaattgcct ccttctttaa ggatcggtcg
    10921 ttgcctgcca acttacaagt gcatccgcga caaagcaatg ttcacccgtt ctatcagcgc
    10981 gctcagctcg tgctgaatct ttcgcatccg gaccagtggg tggaaacgtt cggcatgacc
    11041 attctggaat ccatgagtta tggaataccc gagatcgtcc ccgatatcgg cggacctacc
    11101 gagttggtcg accacggtgt aaatggattt cgcatcaatg tcggtgagct ggaccgtatt
    11161 gcggccacca tccggttcct cgaacagaac aggggagaat gcatccgttt gtccgctcag
    11221 gctcgtatca aagcagggcg tttcacgatc gggaaaatgc agtcggaatt ggggaaagcc
    11281 gttacctgga gcaactgatc gataccgtta tcagcaatca agtcacgaca ggcaccttca
    11341 gggtacctgt tttttttcgc aggtagggtt tttctacgag ccaccaggac atggccgcga
    11401 gtgaataggt tgccgcggca accatccaga aatccgaaag cgtcctggtt tgttgaagtt
    11461 caacgatcgc gttcacggct accatgtggt agatataaac accataggac aaatcctgcc
    11521 gttgcaggat gttgtcggcc agaccgggca aggtataagc agaggaaatg gtcagcaatc
    11581 ccagtacaag ccgggagagg ggcaagcgga aaacatccgg aacgggtgcg cttaccaacg
    11641 aggcgtagac gatgagccag atcaacgctt tgcctttcaa taaaggggat tcgtgcaggg
    11701 cccgccggcg tatccaaatg ccgagcagga acaatgaaaa gtgggggagg aaggtcacct
    11761 gtagccattt ctccgtttga cgtgggatgt ccagtgtcca caacagagcg ctgagcagaa
    11821 agaagaggac cagcagcgct cccagtactt gatcgttttt cgatttgcgc aacaggaccg
    11881 cgtagagcag aggtaggagc aaataaaact gcaattcgac ggtgatcgtc caaaggctgc
    11941 cgttgaggac acccgttcca tacccacgaa gaaattccgg attgaaaaat tgaacaaagg
    12001 aaatttgtcc aagcagccag ggcagcgttt cccatcgcga gaaatccacg tttccaaaac
    12061 attttgcagc gagtacggag atgccgaggc aacaccataa ggccggaaag atgcgcagga
    12121 agcggttggt gaaatagtcg gctggcgact tgctgcgctc cagggatgcg gagacaagaa
    12181 atccgctgat gataaaaaat acaggaacac ccgggaagcg atccagccat tggaaccagg
    12241 ccgattcggt atccaagccg agatggtgaa acgaatgtgc gaccatgacc tggctggccg
    12301 caaggatccg caggaggtcg aagttattgc gggaaaagga gtccgttttc accattcgtt
    12361 cgggcgaaag tatcgaagtt cccttgtatg gactgagttg tgccccctca atcctcgcga
    12421 ttatcaaata gaagtatgcc ttccaggctt ttccggaaat tgaaatagtg gttcagaata
    12481 ggatttgaat cccagattgg gaacacgcgg gtcaggtaga gcccttaaaa tggtcttttt
    12541 tcaaagaaaa ggcccggtgg cacagctatg gcaaggcggg cgcagccaat gaaccgcctg
    12601 attatgtttc gacgctccaa ctttctggcc gctgccatcg tcgtcttgtt gttcctgtgt
    12661 tcaggtcacc cggcatctgc cctgacctat tacagccgga ccagcggggg actctggacc
    12721 aaccccacca cctggtctac tgtaacctac ggtaatgcta ccaacacggg tacgtttccc
    12781 ggtgccggtg acaatgtttt tatcggtgac ggatatacga tctacttcaa caccaatggt
    12841 ggttgcaaca acatcaccat cggacaggga gcatccggcg cgttggagtt ttataacctt
    12901 gcgatctatt ggatgaccgt caccggcgat attaccatca acgccggcgg tcgttttgct
    12961 tatggttaca acagttcgcg tgcgcacatc ctctacatcg aaggagacat cacgaacaat
    13021 ggtaccgtcg atctgtatct ggatgccaac gataatgtgg ccttggttgc gcaagggaat
    13081 actaattctg tgatttccgg taccggttcc tgggatctgt acaccgtgtt gatgtccaag
    13141 atcacttcgc gcagcaacta tcttgaagtg caatcttccg cattcgaaac cgcgatgcgt
    13201 ggccttacgc tcaactatgg tacttatgtt cacaacaaca gcggtacgta caccttcggc
    13261 tccggctccg attacaccat cccgccaaac gtcgcgattg acggtcgtag cggcaccctg
    13321 aatttttcca gcggtgcgac gaacctgaac ctgcaaggtg ccctgatcgt gtcgggcgga
    13381 acggtttata tcgcttcctc caccgggacg ggtggtattc gaaccgacca gggcgtagcc
    13441 ggtgtggttc cctatctgca agtgtcggcc ggacaattgg ttgtttacgg cagtatcagt
    13501 ttccgcaccg cgccttccag ttcctttgcc gaaccattca gctataacca gagcggcggt
    13561 acagtactcg tgaactccgg ttcgaccggc gttacatccg aagtgtttta cgtaaccgat
    13621 caggctacca gctctttcgt catgagcgga ggcacactca acctgcaacg gcctaaccgc
    13681 actggcagct cagtggtgga tatgtttatc tgtgggaaca gtggtacggt taattccact
    13741 ggcggtatcg ttcagtttgg aaatgcatcg acgccggttg gatcgacatt caactttcgg
    13801 ccgttcccaa acgcgaccta tccggctttc cgaatcacag gaccttctgg ttccggcaat
    13861 tcacttcgta cctccaacgc ctccacgtcg aacttccgcc tgctggccat ggttatcgat
    13921 gccggcacca cgttcgatat ccgttctatc gcgggtacgg cgggtaacag caaacgcatg
    13981 agcttgcgcg ggacttttga tggtaattcg gcctggtaca acgacggcac cttcaatccg
    14041 cgttccggtg aagtcgcttt tgaaggcaca gcagcccaga ccctcgacgg tagcaccgat
    14101 acctggtttt acaacatgac gatgaacaat gccgccggtc tgacgatgaa tgcgcgtgca
    14161 tttgtggaca attcattgaa catgacaaac ggtatcattt ataccgatta cacgaatctg
    14221 ctttcggtca gttcttccgg aaacaccact attggaacat catcatctta tatcatcggt
    14281 ccgttcaacc agatctatgc tcaaacttcc gctgctaccg gttactttcc gctgggaaag
    14341 ggagcgaact ggcgaccaat gacgcttaca ccctcacaca cgaccagtga tagtgtgaac
    14401 tacgttggtg agatgatcga atcccctgcc tccggtctcc cttacacgct tccgggatcc
    14461 ttgtctgccg tgtcggcccg tcgttactgg ctggtcacca ataccaacac cggaatcttt
    14521 aacaatgcaa cagcccgact ctactacggt acggacgatg gtgtcaccga ccgactctct
    14581 ctccgtgtag gccaggccat ttctcccaac tgggttaacc ggggcggaac aggaacggcc
    14641 aatggaaccg gaaacatcac ctccagctcg ttcaatgcct ggggagccat ctatacgctg
    14701 gcgaatgcca ccgggggcag caatgccttg ccgatcgaac tggtatcgtt cgttgccgag
    14761 agtcgcgaaa aggatgtcga gctacgctgg accaccgcga cggagatcaa caacgatttc
    14821 ttcaccgtcg aacgttcgcg cgacggattg cacttcgagg ctgtagcaca aaccgccggt
    14881 gccggtaaca cgaccacctt gcagcattac aaggcttacg atctcgcacc cctcaacggc
    14941 acatcgtttt atcgcttgcg acagaccgac ttcgacggcc ggtctaccac ctcggatgtg
    15001 gtgaccgtgt accggaaagg caataccgat ttttccgttt accctaatcc gatcgatgcg
    15061 gatgatgacg tcatgatccg tctgccgcac gacacgaatc gtaatctgct gatccagatc
    15121 aagcgaagcg atggctcgct cgtttttgaa cgcacctatc cggtctggaa tgaacagacg
    15181 attcgcctga gcgggttgcc cttagaagca tccggtttgt atgtgctgtc ggttttcacc
    15241 gacgatggcg gggcttacca gcaaaagctg atggccaaca ttcgttgatt cccaaattga
    15301 gaaacaccta cacctagttg atatgaaaac acctatcctt ctttccgtat tcgcgttttc
    15361 cgcgctgacc tcctttgcca aagggaccgg taagcaggaa cagatcgcaa gcaatttcga
    15421 gcaggagtat cgggtcaatt ccatggaagc gatccgcaac atgggcgatg tcatcttcga
    15481 caaccctgag cttggttccc agcgccaccc cggaaagttt caaccgggat ttccggaggc
    15541 cttgcgcccc tacgatacga acaaagacgg taagctgacg acgaatgaga tcaagccggt
    15601 gatcgaccgg tacctgagtt cgcagaccga attctccagc cgggaaatgg ttcggttcat
    15661 cgactacttc ttctcccaat tctgagatca tccgtgaaac aggaacgcga acaattggcc
    15721 atcatcggct gtgccggcgt cccggcccgc tacggcggct tcgagacctt ggcggaccac
    15781 ctcgtgcgcg agctgcgcag ggattaccgg atcacggtat actgcagcgg cccgatgtac
    15841 cgc
//
LOCUS       DAOTON010000002        11719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_5218_length_11719_cov_3.427298, whole genome shotgun sequence.
ACCESSION   DAOTON010000002 DAOTON010000000
VERSION     DAOTON010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11719
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5218_length_11719_cov_3.427298"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2777
                     /locus_tag="P5292_00060"
     CDS             <1..2777
                     /locus_tag="P5292_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS37545.1"
                     /translation="EALYVTGNYLFRQKKYKKSADWFGRIDMARASQERKDEVNFKTG
                     YGYYMTNDYDKASRSFYAVKDGNSKYASAARYYYAHMAYVNQNYETALKEFLKLRESE
                     AFAPVAPYYITQIYYKQGKYDEILKFAPGALDSNAAKNGMEISRMVAESFYRKEAYAD
                     ALPYLSDYEKNSGAAGRGDWYQLAYCYYRTNRHEAAIPYFQKVIGADDSLTQNAYYHL
                     ADCYLKTNARRSARTSFQSAGKSSFDPEIQEESRFAYAKLSYELNFQSAALEAFRTFT
                     RDFPNSAHVDEANELLVGIYANTRNYKDALTAIEAVKNKTPYIKAAYQKVAYYRGVEC
                     YMDNQLKDAVTLFATSLKYPVDQLLVAEAQYWKGEALYRQNDFDGAVRAYNDYLFTPA
                     AVKSPRYNLANYNVGYANFKRENYSEAQQAFRKYVKDKSQTDPKRYSDALLRVGDCLF
                     MLKDQVGALDYYNQAISSGATSGDYALYQKGVILGVQGRFNDKILTLEKLVEKYPKSV
                     YFDDAVYEIAQANLTIGKNEEALENFRKIITDYPSSSYVKKAELGEALVYYNTRQDER
                     AMAAYKRIVEKYPNSAEARDALVQIKNISVAQNKVDDYLTYVKGVPNADVSIAGQDSV
                     TYEAAELRYTQGNCEDATKDFDQYLKRFPDAIFRVNASYYRSDCLFRSKKYEDALEGY
                     EYVISQPRSPFTEKSLLNAGIIQYRLKQYEKALRYYRTLESVAEVKENIVLAQAGQMR
                     SSFRLNDYSSTIAMTNRVLESGTSDKDLQNEANLLAGKSYFQQDDLGEARKSLSIVAK
                     RTNSEMTAESKYYLAMIEFRLSNYKESQKLIFEIQNQVPSYDYWIAKGFILLGDNYLA
                     QRDTFQAKETYKSIVDNYQKESSDPDDLKAIAQEKLGALEKDTPPVIGEEMNEKLKGE
                     PAVEDTLEIK"
     gene            2790..4469
                     /locus_tag="P5292_00065"
     CDS             2790..4469
                     /locus_tag="P5292_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37546.1"
                     /translation="MHTIKKSLRVSFGWLLLSLLPQAVVAQQEKGDLGDKEYIIVKDY
                     RPVLAESAKISDTPEGDTSTFTAPPMQYNLRSKKAETNYETAVIKAVKVKDEPLPKLY
                     RSLVRGGVGNYTSYLGELYINSLRSKTGSLGLALRHLSASPNLKDAGPADFSDNSAMV
                     YGKYFLENATFDGRIRYDRNVVHYYGYDKNDTVIEKKDSRQLFGKFGVDLGFGSSEIR
                     KDRINYQGRFGFSTLSDNFAASENEIRVGLEAGKQVNDLYVNLPVSMEFFKKKDAEYQ
                     VLSLLSNQSRNIVRLRPEVRIDREKIRARLGLGFELEKNKKSEFHIFPRFDITAPIAD
                     QVLYAFVGADGAVVKNNFHTLAEENPFITSAIIPLNTIEKLNIRAGMRGNFSSTIGFS
                     AGVRYSTIENLQLFYNDLDYFNKFNVLYDDAKVLNLNAEVIYKSSEKFALSLHVDQYN
                     YKMDTEAEAWFRPNTEVALRADYNFRDKILVNTAIFARGPIQARTLDANGAVAEKING
                     WIDANLGVEYKYSKILSGFVRLNNLGFSRYYYWYRYQSMRFNVMAGVAYSF"
     gene            complement(4985..5743)
                     /locus_tag="P5292_00070"
     CDS             complement(4985..5743)
                     /locus_tag="P5292_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRS37547.1"
                     /translation="MSNGTVQHKIADQVSTVTFHHPQSNSLPGALLRELAATIEAAGN
                     DPGSRVIVLQSEGEKAFCAGASFDELVAINDMETGKVFFSGFAAVINAMRKAPKFVIA
                     RVQGKAVGGGVGLASAADYAIAHESASVKLSELAVGIGPFVVGPAVERKIGTAAFGSL
                     SINATEWQSAKWAADRGLYAEVHTSIEGVNEAVTNLANKLANSNPEAMLELKRVLWQG
                     TDHWDQLLLDRAMISGRLVLSDFTRAAIAKFKGK"
     gene            complement(5828..6280)
                     /locus_tag="P5292_00075"
     CDS             complement(5828..6280)
                     /locus_tag="P5292_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37548.1"
                     /translation="MMLYETLDRFEKKFSHLKKKGLRINGLKMTDPKRKKHVIDISRP
                     LVFDNRLLPKSFEGLEVKAVVHGEMPQEFQIDRTQPDWQKREYIWAPERFEHFVDRCS
                     DYIRKQLGNPKMTRDEMLSALAFGDFDAHKEKTSQMIKEGKVPAFPKN"
     gene            complement(6313..8358)
                     /gene="paaZ"
                     /locus_tag="P5292_00080"
     CDS             complement(6313..8358)
                     /gene="paaZ"
                     /locus_tag="P5292_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019620403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HRS37549.1"
                     /translation="MATQLKNYAQGQWIAGASKGMPLYHAITGEHIYTAGSEGLDFKG
                     MHDYARSTGGRALRKMSFHERARMIKALALFLNEKKKDLYAISAATGATKGDSWIDID
                     GGIGNLFVYASKGRRELPDETFLVDGQLEKISKNGTFAGHHICVPLEGVAVHINAFNF
                     PVWGMLEKIAVNFLAGMPAIVKPASATSYLTEAAVRQIVESGILPEGALQLICGSAGD
                     LLDHLDCQDVVTFTGSAETGKMLKRHPSIVEHSIRFNMEADSLNCSILGPDAEPGTEE
                     FNLFVRELSREITIKAGQKCTAIRRAIVPKHLTEAVAAAVSDKLKNTVIGDPSVEGVR
                     MGPLAHKGQVADVKAKVKQLLGACEVVYGDLDAVNVVGADSERGSFMSSMLLLCNEPF
                     RHREPHEVEAFGPVSTLMPYANADEAAELARMGKGSLVGSVFTNDNRFAKTLVLGAAS
                     MHGRMMIVNRECAAESTGHGSPLPHLVHGGPGRAGGGEEMGGVRGVHHYMQRTALQGS
                     PTTLSHILNVYLPGAAITEDRIHPFRKYFEELAIGETLITHKRTVTEADVVNFAGISG
                     DFFYAHTDETSLEGSIFERRVAHGYFVISAAAGLFVDPKKGPVLANYGLDNLRFVQPV
                     YPGDTLQVKLTCMRKTKKDKRDDQVPQGVVEWYVEVLNQRNEVVALETVLTLVACKE"
     gene            8921..9280
                     /gene="rpsF"
                     /locus_tag="P5292_00085"
     CDS             8921..9280
                     /gene="rpsF"
                     /locus_tag="P5292_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRS37550.1"
                     /translation="MLNQYETVFIATPVLSEEQLMDAVNKFRKILTDHGSEIVHENNW
                     GLRKLAYPIQKKNTGFYYLLEYKGPGEVISKLETEFKRDERIMRFLTVALDKHAIAYN
                     VKKRKNAELKKQEEQTA"
     gene            9293..9562
                     /gene="rpsR"
                     /locus_tag="P5292_00090"
     CDS             9293..9562
                     /gene="rpsR"
                     /locus_tag="P5292_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRS37551.1"
                     /translation="MAASANEVRYLNPPAVDTNKKKYCRFKKNGIKYIDYKDATFLIK
                     FVNEQGKMLPRRITGTSLKYQRKVAQAVKRARHLALMPYVSDMLK"
     gene            9578..10024
                     /gene="rplI"
                     /locus_tag="P5292_00095"
     CDS             9578..10024
                     /gene="rplI"
                     /locus_tag="P5292_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRS37552.1"
                     /translation="MEIILKQDVDNLGYADEVVKVRPGYARNFLIPRGLAVVATEANR
                     KMLAETMKQRSHKAAKIKGDAESVAKQLEAMTLKIGAKVGENNKIYGSVTALQIADAL
                     AKHGVNVDRKKVQLDTDHIKTLGTYTALVNLHKDVKAKVNFEVVAE"
     gene            complement(10135..10962)
                     /locus_tag="P5292_00100"
     CDS             complement(10135..10962)
                     /locus_tag="P5292_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRS37553.1"
                     /translation="MSSLAAQFERDGFLVLPDFHPAAACRELMERGRHLSENYRLDGK
                     GSVFQTSEQTKTSDAYFLESGERISFFFEKDAFDETGALKGDLFRSLNKIGHALHDLD
                     PVFDRFSRSPQLKALSASLGMQDHLLIQSMLIFKHARIGGVVDVHQDSTFLYTEPDSC
                     VGFWFALEDATIENGCLWAKPGGHRTSLRSRFARDGKGGTRFLELDPAPLETDGMQAL
                     EVKQGTCIVLHGRLPHFSQPNTSGKSRQAYAIHTIDPTAHYPMDNWLQRDRRALKGF"
     gene            complement(11018..11557)
                     /locus_tag="P5292_00105"
     CDS             complement(11018..11557)
                     /locus_tag="P5292_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRS37554.1"
                     /translation="MHVRSTHLFIAASLDGFIADANDDLSFLSKVEAPGEDYGYTAFM
                     EKVDTVVMGRRTYDKVMSMGIPFPHADRSCYVITRTPRAAIGRTIFHTGDPAALIREL
                     RAKPGGIIFVDGGAQVVNRLLQERLIDGITLSIIPVLLGDGIRLFSEGGLMQDLELLD
                     HRAYPSGLVQLRYRIKPGS"
BASE COUNT         2710 a         3278 c         3265 g         2466 t
ORIGIN      
        1 aggaggcgct ctatgtgacc ggcaattatc tcttccgcca gaagaagtac aagaagtcgg
       61 ccgactggtt cggccggatc gacatggccc gcgcgagtca ggagcgaaag gatgaggtga
      121 acttcaagac cggttacggg tattacatga cgaacgatta cgacaaagcc tcccgttcgt
      181 tctatgccgt gaaggatggg aacagcaagt acgccagtgc cgcgcgctat tactatgccc
      241 acatggccta cgtcaaccag aactatgaaa cggcgctgaa ggaattccta aagctccgcg
      301 aatcggaagc ctttgccccg gtagcgcctt actacatcac gcagatctat tacaagcagg
      361 gcaagtacga cgagatactc aagttcgcgc ccggggcact cgactccaac gccgcgaaaa
      421 acggtatgga gatctcccgc atggtcgccg aatcgtttta tcgcaaggaa gcctatgcgg
      481 acgctttgcc ttacctctcg gattacgaga agaactcggg tgccgccggc cgtggcgact
      541 ggtaccaatt ggcctactgt tattatcgca ccaaccggca cgaagcggcg atcccgtatt
      601 tccaaaaagt gatcggagcc gacgactccc tgacgcagaa tgcttactac cacctggcgg
      661 actgttatct caaaaccaac gcaagacgca gcgcgcggac gtcctttcag tctgccggca
      721 aatcttcctt cgatccggag atccaggaag aatcacgctt cgcctatgcc aagctttcct
      781 atgaactgaa tttccagtcg gccgcgctcg aagcgttccg gacgttcacg cgtgatttcc
      841 cgaacagcgc ccacgtggac gaagcgaacg aattgctcgt cggcatctat gccaatacgc
      901 gtaactacaa ggacgcgctc acggctatcg aagccgtgaa gaacaagacg ccttatatca
      961 aagccgctta tcagaaagtg gcttattacc ggggtgtgga gtgttacatg gacaaccaac
     1021 tgaaagatgc ggtcacactc ttcgctactt cactgaagta tcccgtcgac cagttgttgg
     1081 ttgcggaagc ccagtactgg aaaggggaag ccttgtatcg tcagaacgat ttcgacgggg
     1141 cggtgcgggc gtataacgat tatctcttta cgccggccgc ggtcaagtcg ccgcgctata
     1201 acctggccaa ctacaatgtc ggttacgcca atttcaagcg cgagaattat tccgaagccc
     1261 aacaggcgtt ccggaagtac gtgaaggaca aatcccagac cgacccgaaa cgctacagcg
     1321 atgccctcct gcgggtgggc gattgcctgt tcatgctgaa ggaccaggtc ggcgccttgg
     1381 attattacaa ccaggccatt tccagcggcg ccacatcggg cgactacgcg ctgtaccaga
     1441 agggcgtgat cctcggtgtg caaggccgtt tcaacgacaa gatcctcacg ctcgagaagc
     1501 tcgtcgagaa gtatcccaag tcggtgtact tcgacgacgc cgtttacgag atcgcacagg
     1561 ccaacctgac gatcgggaag aacgaagaag cgctcgagaa cttccggaag atcatcaccg
     1621 attatccgag cagcagctac gtgaagaagg cggagttggg ggaggcgctg gtgtactaca
     1681 atacccgtca ggacgaacgt gccatggccg cctacaagcg gatcgtggag aaatatccga
     1741 actccgccga agcgcgcgac gccctggtgc agatcaagaa catctcggtg gctcagaaca
     1801 aagtggacga ctacctcacc tatgtgaaag gcgttccgaa cgcggatgtg tccatcgccg
     1861 gacaggacag cgttacttac gaagccgccg agctgcggta cacccagggc aactgcgaag
     1921 acgcgacgaa ggacttcgac cagtacctga agcgtttccc ggacgcgatc tttcgggtca
     1981 acgccagcta ctatcgctcc gattgtctct tccggagcaa gaagtacgaa gacgcgctgg
     2041 aaggttatga atacgtgatc tcccaaccgc gttcaccgtt cacggaaaag tccctgctca
     2101 atgccggtat catccaatac cgcttgaagc aatacgagaa agccctgcgg tactaccgta
     2161 ccctcgagtc ggtcgccgag gtgaaggaga acatcgtcct cgcacaggcc gggcaaatgc
     2221 ggtccagttt ccggctgaac gactattcat cgacgatcgc catgacgaat cgtgtcctgg
     2281 aatccggtac atccgacaag gacctgcaga acgaggcgaa cctcctggcc ggtaaatcct
     2341 atttccaaca ggacgatctg ggagaagcca ggaagtcgct cagcatcgta gcgaagcgca
     2401 cgaacagtga aatgacggcg gagagcaagt actacctcgc catgatcgag ttccggttgt
     2461 cgaactataa ggaaagtcag aagctgatct tcgagatcca gaaccaggtg ccgtcgtacg
     2521 attactggat cgccaagggc ttcatcctgc tgggtgataa ctacctcgcg caacgcgaca
     2581 ccttccaggc gaaggagacc tacaagagca tcgtcgataa ttatcagaaa gagtcgtcgg
     2641 atcccgacga cctcaaggcg atcgcccagg agaaactcgg ggcactggaa aaggatacgc
     2701 ctccggtcat cggagaagaa atgaacgaga aattgaaggg cgagcccgca gtagaagata
     2761 ccctggaaat caaatagcac gataccacta tgcatactat caagaaaagc ttgcgcgttt
     2821 cattcggatg gctgttgctc tcgctgctgc cgcaagcggt ggtcgcgcaa caggaaaagg
     2881 gcgacctcgg tgataaggag tatatcatcg tcaaggacta tcgtccggta ctcgcggaat
     2941 cggcgaagat ctccgacacg cccgaaggcg atacctccac ctttacagcc ccgcccatgc
     3001 agtacaacct gcgctcgaag aaagcggaaa cgaactacga aaccgcggtg atcaaagcgg
     3061 tgaaggtgaa ggacgaacct ttaccgaaac tgtatcgctc gctcgtgcgc ggaggtgttg
     3121 ggaattacac atcctacctc ggcgaactct acatcaactc gctgcgctcc aagaccggtt
     3181 ccttaggtct cgccctgcgt cacctctccg cctcgccgaa cctgaaagat gccggccccg
     3241 ccgatttcag cgataactcg gccatggtgt acgggaagta cttcctggag aacgccacct
     3301 tcgatggccg gatccgctac gaccggaacg tggtgcatta ctacggctac gacaagaacg
     3361 ataccgttat cgaaaagaaa gactcccgcc agctgttcgg gaagtttggc gtggacctgg
     3421 gcttcggctc cagcgagatc cggaaggatc gcatcaacta tcagggccgc ttcggcttct
     3481 cgaccctgag cgacaacttc gctgcctccg agaacgagat ccgcgtcggc cttgaggccg
     3541 gtaagcaggt gaatgatctg tatgtcaacc tgccggtatc catggagttc ttcaagaaga
     3601 aggatgccga gtaccaggtg ctctccctgc tctcgaatca gagccggaac atcgtccgcc
     3661 tgcgacccga agtacggatc gatcgcgaga agatccgcgc ccggctcggt cttggcttcg
     3721 agctggagaa aaacaagaaa tcggaattcc acatcttccc gcgcttcgac atcacggcgc
     3781 cgatcgccga ccaggtgctg tatgccttcg ttggcgctga tggcgcagtc gtcaagaaca
     3841 acttccatac gctggcggag gagaatccat tcattacctc agcgatcatt ccgcttaata
     3901 cgatcgaaaa gctgaacatc cgggcaggta tgcggggcaa tttctcttcg accatcggct
     3961 tttcggccgg cgtccgttac agcacgattg agaacttgca gctcttttac aacgacctgg
     4021 actacttcaa taagttcaat gtgttgtacg acgatgccaa ggtgctcaat ctgaatgcgg
     4081 aagtgatcta caaatcatcc gagaagtttg cgctctccct gcacgtcgac cagtacaatt
     4141 acaagatgga caccgaggcc gaagcctggt tccggccgaa taccgaagtc gcgctgcggg
     4201 ccgattacaa cttccgggac aagatcctgg ttaacaccgc catcttcgca cgcggcccca
     4261 tccaggcccg tacactcgat gccaacggtg ccgtagccga aaagatcaac ggctggatcg
     4321 acgccaacct gggagtggag tacaagtaca gcaagatcct ctccggattc gtccgcctga
     4381 acaacctcgg cttcagccgc tactactact ggtaccgcta ccaaagcatg cgcttcaacg
     4441 tcatggccgg tgtggcgtat tcgttttgag cggtgcgatg tgtgttgatg ggagggttta
     4501 gcgaatagcg aatagccaat agcgaatagt gaatcttgcc cgccgtagct ttagcgtagg
     4561 tgggagtaaa cagtgaacag taaacagtaa acagcattgt ggtctctgat aaatcctcaa
     4621 cctgagctaa ttcattactt ccgcgttgta aaaagaggcg ttcctgtgga cgcctctttt
     4681 ttattggagt tgaccggctg ccggagaaat ggcagcaaca agaagcggaa tgggtgccat
     4741 gtattcgaaa ccggcaatca tgtattcgaa aaagaactgc taataaaaat gttacatcaa
     4801 aaaaagttga attaagcgag tgtcaaagga taaatttata aatcactaat cacaaatcac
     4861 aattcgcttc cgccaacgct aatgctacgg cgggcaagat tcactattcg ctattcgcta
     4921 ttcactgttc actgtttact gttcactctt cactattcac tattcactgt tcactccccc
     4981 ctcctcactt ccccttaaac ttcgcaatcg ccgccctggt aaaatccgaa agcaccaacc
     5041 gcccgctgat catggcgcgg tcgaggagca attggtccca gtgatcggtg ccttgccaga
     5101 gtacccgctt caattcgagc atggcttccg ggttcgaatt ggccagtttg ttggcgaggt
     5161 tggtgaccgc ttcattcacg ccttcgatcg aagtgtgtac ttcggcatac aacccgcgat
     5221 cagcagccca tttggccgat tgccactcgg tcgcgttgat cgacaacgag ccgaaggccg
     5281 ccgtgccgat ctttcgctcc actgccggac ctaccacgaa cggtccgata cccacggcca
     5341 gttcgctcag cttgacggac gcgctttcgt gcgcgatcgc gtagtcggct gcggaagcga
     5401 gtccgactcc gccacccacc gctttcccct gaacccgtgc gatcacgaac ttcggcgcct
     5461 tgcgcatggc gttgatcacc gcggcgaaac ctgaaaagaa gaccttgccg gtttccatgt
     5521 cgttgatcgc aaccagttcg tcgaacgaag cgccggcgca gaacgctttc tctccttcac
     5581 tttgcaggac aatcacgcgc gagcccggat cgtttcccgc ggcttcgatc gtagccgcca
     5641 gttcgcgcag caaggcaccc ggcagggagt tgctttgcgg atggtgaaag gtgacggttg
     5701 atacctggtc ggcgatcttg tgctgtacgg ttccgttgga cattggatag aggagtagga
     5761 ggagtgggta aagagaaacc gcggccagga atgtcggccg cggtcggagg attacggatt
     5821 gaacgaatca attcttcgga aaggccggga cttttccttc tttgatcatc tgggaggtct
     5881 tttccttgtg cgcgtcaaag tcgccgaagg cgagcgccga caacatttcg tcgcgggtca
     5941 tcttcgggtt gccgagttgt ttgcggatgt aatcgctgca acgatcgacg aaatgttcga
     6001 aacgctccgg cgcccagatg tactcgcgtt tttgccagtc gggctgggtg cggtcgatct
     6061 ggaattcctg cggcatctcc ccgtgcacta ccgccttcac ctcgagcccc tcgaagcttt
     6121 tcggtagcag gcggttgtcg aacacgagcg gacgactgat gtcgatcacg tgtttcttgc
     6181 gtttcggatc ggtcatctta aggccgttga tacgaaggcc tttcttcttc aggtgcgaga
     6241 attttttctc gaagcggtca agggtttcgt aaagcatcat ggcggtgtgg attgaagggt
     6301 gggggagtgg agttattctt tacaggcgac cagggtgagc acggtctcca gcgcgacaac
     6361 ctcgttgcgc tggttgagta cttctacata ccattcgacc acgccttgcg gcacctggtc
     6421 gtcacgtttg tcttttttcg ttttccgcat gcaagtcagt ttcacctgca gcgtgtcgcc
     6481 cgggtaaacg ggttgtacga agcggaggtt gtccaggccg tagttggcca gcacaggtcc
     6541 ctttttcgga tcgacgaaca atcccgcggc cgcggaaatg acgaagtatc cgtgcgcgac
     6601 gcgccgctcg aagatggatc cttccagcga ggtctcgtcg gtatgggcat agaagaagtc
     6661 gccgctgatg ccggcgaagt tcacgacatc agcttcggta acggtacgct tgtgcgtgat
     6721 cagggtttcg ccgatggcga gttcttcgaa gtatttgcgg aacgggtgga tgcggtcttc
     6781 ggtgatcgcc gctccaggga gataaacatt caggatgtgc gagagcgtgg tcggggaacc
     6841 ctgcaaggcc gtgcgctgca tataatggtg tacgccgcgg acgccgccca tttcttcgcc
     6901 tccaccggcg cgaccggggc cgccgtgtac gaggtgcggg agcggagaac cgtgtcctgt
     6961 cgactcggcc gcgcactcgc ggttgacgat catcatccgc ccgtgcatgc tggccgctcc
     7021 gagtacgagt gttttggcaa aacggttgtc gttcgtaaag acggaaccga ccaggctgcc
     7081 tttacccatg cgggcaagtt cggccgcttc gtcggcattg gcatacggca tcagcgtgct
     7141 gaccggaccg aaggcttcca cctcgtgcgg ctcgcggtgg cggaagggtt cgttgcacag
     7201 caacagcatg ctcgacatga acgatccgcg ctcgctgtcg gcgccgacca cattcaccgc
     7261 atccaagtca ccatacacga cttcgcaggc gccgagcagt tgcttcactt tcgctttcac
     7321 atcggccacc tggcctttgt gagccagcgg tcccatgcgg acgccttcca cggaaggatc
     7381 tccgatcacc gtattcttca acttgtcgga tacggcggcg gcgacggcct ccgtcagatg
     7441 cttcggtacg atggcgcggc ggatggccgt gcatttttgt ccggccttga tggtgatctc
     7501 ccggctgagc tcgcggacga agaggttgaa ttcttccgtt ccgggttcgg cgtcggggcc
     7561 gaggatggag cagttcaacg aatccgcctc catgttgaag cggatcgaat gttcgacaat
     7621 ggatgggtga cgcttcagca tcttgccggt ctccgcggag ccggtgaagg tgacgacatc
     7681 ctggcaatcg agatgatcga gcaggtcgcc cgcgcttccg cagatgagct ggagtgcgcc
     7741 ttcgggcagg atgccggatt cgacgatttg acggaccgcg gcttcagtga ggtaggaggt
     7801 ggcgctggcg ggcttcacga tggccggcat gccggccagg aaattcaccg cgattttttc
     7861 cagcataccc cacacaggga agttaaaggc gttgatgtga acggccacgc cttcgagcgg
     7921 tacacagata tggtgtccgg caaacgtccc gttcttcgat atcttttcga gctgaccgtc
     7981 gacaaggaac gtctcgtccg gcagttcgcg acggcctttg ctggcataca cgaagaggtt
     8041 gccgatgccg ccgtcgatat cgatccagga atcgcctttg gtcgcgccgg tggcggcgga
     8101 tatggcgtat aggtccttct tcttttcgtt caggaataag gcgagcgctt tgatcatgcg
     8161 cgcgcgctcg tgaaagctca tcttgcgcag tgcgcgacca cccgtcgaac gggcataatc
     8221 gtgcatgcct ttgaaatcga gcccttcact ccctgcggta tagatgtgct cgcccgtaat
     8281 ggcatggtac agcggcatgc ctttactggc gcctgcgatc cattgccctt gggcgtagtt
     8341 cttaagttgc gttgccattg aatcaagaaa gcaaacaccc tccgcccgcc tgtagttgga
     8401 ggcagtagtg tattgctcta actgagtttc cggttcactc cgcttccccg tggagtcgac
     8461 agttcgtcga tggtattggc ctgttcaggc ggtttccgga agtaattatc ccgctacaaa
     8521 gataacccga actgaaaggg agtcaaggga ttgggagaca atagttcagg gtaaaggcgg
     8581 gtttgaggtt aatggacggt tttgggacga ggggcggcga aagggaacta gggactgaag
     8641 gctggttacc agatgctgga gattaaacta ttaacactcc aaatagctga tattcaggca
     8701 aaaaaatgaa taaaggtcca gctcgtcgtg ctcaattcac ccttcctcgc cctcggtctc
     8761 gttcctcgca accaattccc ttcctcggtc ccggtctcgt tcctcgtaac aattctctcc
     8821 cgcctgttga gccttttccc accccccact tgcacattcc gcttcgtttt cctacatttg
     8881 caaccccttg tacaaaaacc ccaagggtca ctaaaccaac atgttaaacc agtacgagac
     8941 ggtcttcatc gctacgcctg ttctttcgga agaacagctg atggacgccg tcaacaaatt
     9001 ccgcaaaatc ctcaccgacc acggttccga gattgttcat gagaacaatt ggggattgcg
     9061 caagctggct tacccgattc aaaagaagaa caccgggttc tattacctgc tcgagtacaa
     9121 aggtccgggc gaggtaatct ccaaactgga gacggagttc aaacgggatg agcggatcat
     9181 gcgcttcctc actgtggcac tggacaaaca cgcgatcgcg tacaacgtga agaagcgtaa
     9241 gaacgctgaa ctcaagaaac aggaggaaca aaccgcttaa tcgaggaaca acatggcagc
     9301 atcagcaaat gaagtccgct acctcaaccc cccggcggtg gacaccaaca agaagaagta
     9361 ttgccgcttc aagaaaaacg gcatcaagta cattgattac aaagacgcga ccttcctgat
     9421 caagttcgta aacgaacagg gcaagatgct tccgcgccgc attaccggta cttccctgaa
     9481 gtaccagcgt aaggtggccc aggcagtaaa gcgtgcacgc cacctggcac tcatgccgta
     9541 cgtgagcgat atgttaaaat aagaaggagg aacgcacatg gaaatcatcc tgaaacaaga
     9601 cgtcgacaac ctcggctatg ccgacgaagt cgtaaaggtg cgtcccggtt acgcccgtaa
     9661 cttcctgatc ccgcgcggcc ttgccgtggt ggcgaccgaa gccaaccgca agatgctggc
     9721 cgagacgatg aagcagcgct cgcacaaggc cgccaagatc aaaggcgacg cagaatccgt
     9781 agccaagcaa ctcgaagcca tgacgctgaa gatcggcgcc aaggtgggcg agaacaacaa
     9841 gatctacggt tccgttaccg ccctgcagat cgcggacgcg ctggccaagc acggcgtgaa
     9901 cgtcgaccgc aagaaagtcc agctcgatac cgaccacatc aagaccctcg gtacctatac
     9961 cgcacttgtc aacctgcaca aggacgtaaa ggccaaggtg aacttcgaag tcgtggccga
    10021 gtaatacgac ctgacctgac caacagaggc cgtcccgaaa ggacggcctc ttgctttttc
    10081 agagtgcctg accgaatcgt ctgggttcac cgcggacgga gaagtcggcg attttcaaaa
    10141 tcccttcaac gccctacggt cgcgctgcag ccagttgtcc atcggatagt gggctgtcgg
    10201 atcgatggtg tggatcgcat acgcctggcg cgatttgccg ctggtattcg gctgactgaa
    10261 atgcggcagg cgaccgtgga ggacgatgca ggtgccctgc ttcacttcga gtgcttgcat
    10321 cccgtccgtc tcgagcggcg cgggatcgag ttcgagaaaa cgcgttccgc cctttccgtc
    10381 gcgcgcgaag cgcgatcgca ggctggtacg atgcccgccc ggcttggccc agaggcagcc
    10441 gttttcaatc gtcgcgtctt cgagcgcgaa ccagaacccg acgcaggaat ccggttcggt
    10501 atacaggaag gtgctgtcct ggtgcacgtc gaccacgccc ccgatgcggg cgtgtttgaa
    10561 gatcagcatg ctttggatga gcagatggtc ctgcataccc aacgaagccg aaagtgcttt
    10621 cagttgcggc gatcgcgaga agcggtcaaa gaccgggtcc aggtcatgca aggcatgacc
    10681 gatcttgtta agcgaacgaa agaggtcgcc ttttaaagcg ccggtctcgt cgaaggcgtc
    10741 tttctcgaag aagaaactga tgcgctcgcc gctctccaga aaatacgcgt cgctggtctt
    10801 ggtctgttcc gaggtctgga agaccgaacc ttttccgtcc agccgatagt tttcggacaa
    10861 gtgtcgtcct cgctccatca gttcccggca ggccgcggcc gggtggaagt cgggtaagac
    10921 gaggaacccg tcgcgttcaa actgcgcagc aagtgacgac atcgtgtcaa ggtagtgttt
    10981 gagcggcgct ttacctaagc cggagctgga agtcgcatca actgcccggc ttgatccggt
    11041 aacggagctg taccagtccc gagggatagg cgcgatggtc caggagctcc aggtcctgca
    11101 tcaatccccc ctcggaaaag agccgtatac cgtcgccgag caggacggga atgatcgaca
    11161 gtgtgatgcc gtcgatcagg cgttcctgca acaaccggtt cacgacctgc gcgcctccgt
    11221 ccacgaagat gatacctccc ggcttggccc gcagttcccg gatgagtgcc gcgggatcgc
    11281 cggtatggaa aatcgttcgc ccgatcgccg cgcgaggcgt gcgcgtgatg acgtagcagg
    11341 aacggtccgc atgcggaaac ggaatgccca tgctcatgac cttatcatag gtgcgacgac
    11401 ccatcaccac cgtgtccacc ttctccatga aagcggtata tccataatcc tccccgggcg
    11461 cttccacttt gcttagaaag ctgagatcgt cgttcgcatc cgcgatgaaa ccgtcgaggg
    11521 aagcggcgat gaagaggtga gtggagcgta cgtgcatgag gtagtaaagt gtcgggttag
    11581 attgtgttta tcgaaaatat aagatgaata agtatgcaag tatagaaaag tcaggcgtgt
    11641 gcttcgttaa attttccgaa acatttgcat ttttaaaatt cattgttatc ctttgatatt
    11701 gctaacgatt ttaaaccga
//
LOCUS       DAOTON010000003        11252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_5459_length_11252_cov_3.136554, whole genome shotgun sequence.
ACCESSION   DAOTON010000003 DAOTON010000000
VERSION     DAOTON010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5459_length_11252_cov_3.136554"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="P5292_00110"
     CDS             <1..810
                     /locus_tag="P5292_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37555.1"
                     /translation="PDDAAIVKFDVNGNFAGFFGYDGLGTGNDQAINLTHDASDNIIA
                     TFRTDVDPDPILANYNILTIKFDSNLNQVWTSPPEYNSPVNGDDLPIDLAVNSSGDVF
                     VVGASEADTIAARVNRNWVIIRYTPAGTQSWVTTVDGTGADDDEPNAVALRGTSLWVT
                     GYTVGAGTQKDLTVNRYDISTSISFPDRVDIMLSAFPNPMTASATLDFAKPLTAGSVL
                     EVSDITGKLVQRRSVSGSRVLIERNEWDSGLYLVSVSTPDGDRYLAKLIVQ"
     gene            819..2402
                     /locus_tag="P5292_00115"
     CDS             819..2402
                     /locus_tag="P5292_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HRS37556.1"
                     /translation="MRASLLSFILLLTLTTAGFAQRNLSIRSTLQIPGQTLAGCWHYE
                     DGSGKIYALVGAAQGIIIIDITDPSSPQQLFQLPGASSLWHEVKVQGDYAYAVSEGVD
                     SLGIKNGLQIIDLRFLPDSAPNKFWQGDGAWAGQLLTGHTVTTAGNFVYVNGHNLSSL
                     GRGVFIADISDPWNPTYVGAVTSRYCHDSYVRGDTIWTSDIIDGLFSVYDITNRQNPI
                     LLATQQTPGQFNHNSWLSDDGRTLFTTDERSNEPLAAFDVSDLSNISLLDEFYNENMS
                     SAEVHNIRVINDYLINPSYGSQLTIADASDPENLIEVANFPTGSSLCWDADPYLSNGL
                     IIATDMSSGIFYVFEPNYVRAARLEGTVTDSVSGLPLSDVAVELLSTTRPVRNTNLLG
                     KYKTGIVDAGAYTVRFSKAGYQTKDVTGINLVNGSLLTLDVQLVSSSIGIEEHDQYAA
                     LVAFPSPVDEFLNIRLASNAPVDWMISDSQGRLVSQGKMVPASSSLVLPVNELAQGVY
                     SIRLSQSSQLRQGRFIVAR"
     gene            complement(2403..3281)
                     /locus_tag="P5292_00120"
     CDS             complement(2403..3281)
                     /locus_tag="P5292_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRS37557.1"
                     /translation="MSKIPESELILNSDGSVYHLNLHPEEIADTVINVGDPDRVGMVS
                     KFFDSIEIRKQKREFVTHTGTYKSKRITVLSTGIGTDNIDIVYNELDALVNIDLRKRE
                     IQPQLKSLNLIRIGTSGSLQKEIPVDGFVFSRFGLGLDGLLNFYRLKNDQEEQQIVEA
                     FRHHYPNEGILPQSYLARCSNKLEMALSEGMFKGITASCSGFYAPQGRVLRYELARPN
                     FIETLHSFRLGEHRITNFEMETGAMYGLARILGHHCCSINAIVANRITNEHTHKGDET
                     MNRLIETVLDRIAAGL"
     gene            3324..4190
                     /locus_tag="P5292_00125"
     CDS             3324..4190
                     /locus_tag="P5292_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRS37558.1"
                     /translation="MAQPKNHILVPIDFSEQSLIALGQSYNLARLNRADLTLIHIIDE
                     SFHLPFMGKKEDKSMEKKIQKELDKLASETSQTAGIKVNTMVTRGKIYEEIQKAVKKL
                     KVSLIIMGTNGSVGFKRFIGSNALRVIREASCPVITIKGKKHRAGCKHIVLPLDLQKE
                     TKEKVHNAIEFSKLFGSTIDLVSVLTTDDEFIVNKLKRQMHQVHEYILEQNIPCTVEF
                     LHGDDVAEEVVKYAKRIKADIIMVMTQSEMDWSDMFISSATQDIINSSDIPVLSVHPK
                     EKKDTTLTPFEY"
     gene            4207..5076
                     /locus_tag="P5292_00130"
     CDS             4207..5076
                     /locus_tag="P5292_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRS37559.1"
                     /translation="MHSKNFQIKRILIPYDFSETADLALEHAVFMAKLHKADIVLLHV
                     IETYSFTSAISHAFSKSQSEFESKIESSASDKLHHDSGMGVQFRTEMGKIYKTIISVA
                     EETDADLIVMGTHGVSGFQEFLVGTNTYRVVVGAPCPVVSVQTHARKIGFKNIVLPID
                     NSPASRQKVKHAVEIARHYNSIVHILGIMTMSDTDMQRRFELKVHQVADYLAEHEIPY
                     TTKVFKGDNTAQITMDYATQCSADLIVIMTEQEGGGLFMGNFAQQIVNHSKIPVMSIR
                     PDEGDPDKISIGY"
     gene            complement(5141..5857)
                     /locus_tag="P5292_00135"
     CDS             complement(5141..5857)
                     /locus_tag="P5292_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRS37560.1"
                     /translation="MRRLLSLLLLALPLAGCSQIDLKKLEKSVNETVQGSKPLSESEV
                     VAGLKEALQVGSRNAAGSAAKTDGYFGNNLIKIPFPPEAKSMETKLRSLGMNKQVDDF
                     ILTVNRAAEEAAKEAAPVFLDAITGMTVTDGFNILRGADTAATGYLRQKTSGALHTKF
                     KPIIQRAIEKVSVTRYWNPLATTYNKIPFVDPVNPNLEEYITQRALSGLFTLVAQEET
                     KIRRDPAARVTALLKRVFGN"
     gene            complement(5913..6227)
                     /locus_tag="P5292_00140"
     CDS             complement(5913..6227)
                     /locus_tag="P5292_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37561.1"
                     /translation="MVGLVVFGALAGWVVMTLWNWLIPALFTGPVIGFWQALGILVLS
                     KILFGSAKGWKSGGGHCRDGSWKERWEGMSPEERERVRSLWKQRCRKSTPPPAPPAET
                     ES"
     gene            complement(6269..6832)
                     /locus_tag="P5292_00145"
     CDS             complement(6269..6832)
                     /locus_tag="P5292_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRS37562.1"
                     /translation="MTQSQDESIRTALRENGRRLRDFIRSRVAEPEDADDIFQDVVLE
                     LTSAYRLMQPIEKMAAWLFRVARNKITDRYRRKKSVRLEDYFRFPDPSGEETLLVDEL
                     LRSDTAGPDAEFDRSLLLQAIESALDELPVEQREVFIAHELEGKSFQQLSDESGLSIN
                     TLLARKRYAILKLRERLQEYYDELNTP"
     gene            6942..8198
                     /locus_tag="P5292_00150"
     CDS             6942..8198
                     /locus_tag="P5292_00150"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRS37563.1"
                     /translation="MLAEIITIGDELLIGQVVDTNSAWIGQRLNEIGVRIKQITSVSD
                     NEEHILKALDEAGQRSGIILITGGLGPTKDDITKKTLAKFFKAGMRLDGPSLANVERI
                     FRARGREVTELNRRQAEVPENATALLNPLGTAPGMWFEEHGRIYVSLPGVPHEMKGLM
                     EQEVIPQLRQRFKLPPVIHRTILTQGIGESMLSDLLEPWEDALPPHIRLAYLPADGRV
                     RLRLTASGKSEAEINAELDIQVAGLKNLANRYIYGYEEETMEGVVGALFRAKGWKVAT
                     AESCTGGYLSHRLTLVPGSSDYYMGSVIAYDNAVKTSLLGVDPKLFEEVGAVSEPVVR
                     VMAENVRKLLGTELGIAISGIAGPSGGTEEKPVGTVWIAVSSPAGTLSKRFQLGQFRE
                     RVIREAAQHALAMTRRILLAEPEPEN"
     gene            8365..8598
                     /gene="rpmB"
                     /locus_tag="P5292_00155"
     CDS             8365..8598
                     /gene="rpmB"
                     /locus_tag="P5292_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRS37564.1"
                     /translation="MARICDLTGKRAMGGHHVSFSNKKSKRKFNPNLQVKRFYVPEMG
                     EWITLKVSTSALRTIDKMGIGPAIEKAIKKGKI"
     gene            8672..10096
                     /locus_tag="P5292_00160"
     CDS             8672..10096
                     /locus_tag="P5292_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HRS37565.1"
                     /translation="MRVVSIVLWLLIGLPVASLLAQDSDRYRNEQDFLTPEFYKARRE
                     ALRQLIPEGSCAVLLSAPVRNRSNDVDYPYHQSPDFYYLSGWTEPNSVLVVFKEAQRF
                     NKGEVKDILLVPPRDPAKERWNGRRGGKTAARRISGADWVLTTESWDTLQLPFESQTK
                     VLQLRLEDPRIAGNQDPLEYDGLLRSYRKKVDSASVSPDDFLLKRALTTMRMVKHPDE
                     LALLQRAVDISVSGHLEMMKALEPVMTEYQVEAVGEYVFHALGAEAVGYPSICGGGEN
                     STVLHYTDNRRPLGAGDLILLDMGAEYHGYTADITRTLPVTGKYTPEQLAIYNLVLEA
                     RDSGIAACQPGNPFQAPNAAARRVISRGLQDLGIIQSADELKTYFMHGTSHYLGLDVH
                     DAGNYGPLAPGNVITVEPGIYIAADSPCDRKWWGIGIRIEDDILITTTGHQNLSEKLP
                     VKPEEIEKAMKQPAILNNSALKNE"
     gene            10178..10360
                     /gene="rpmG"
                     /locus_tag="P5292_00165"
     CDS             10178..10360
                     /gene="rpmG"
                     /locus_tag="P5292_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRS37566.1"
                     /translation="MAKKGNRVQVILECTEHKTSGQPGTSRYITTKNKKNTTERLELK
                     KFNPILKKMTVHKEIK"
     gene            10387..10542
                     /locus_tag="P5292_00170"
     CDS             10387..10542
                     /locus_tag="P5292_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HRS37567.1"
                     /translation="MAKKVVATLKTGKGKEFSKVIKMVKNPDTGSYQFKEEVVHNDHI
                     KDFLKEK"
     gene            10624..>11252
                     /locus_tag="P5292_00175"
     CDS             10624..>11252
                     /locus_tag="P5292_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HRS37568.1"
                     /translation="MGLFSFFSKEKKESLDKGLEKTKTSLFSRIAKAVVGKSTVDAEV
                     LDNLEEILVSSDVGVNTTLRIIKRLEERVARDKYVGTEELNRLLKEEITGLMAENNTA
                     DATEFSLPDTGGQPYVIMVVGVNGVGKTTTIGKLAYHFKKAGKKVVLGAADTFRAAAV
                     DQLDIWSKRVDVPIVKQGMGSDPAAVAYDSVQSGVAQEADVVIVDTAGR"
BASE COUNT         2760 a         2913 c         2958 g         2621 t
ORIGIN      
        1 ccggacgacg cggctattgt caaattcgat gtaaacggga attttgccgg cttcttcggc
       61 tatgatggtt tgggaacagg aaacgaccag gcgatcaacc tgacccacga cgcttccgat
      121 aacatcattg ccacgttccg gacagatgtt gatccggacc cgatcctcgc gaactacaat
      181 attctcacga tcaaattcga cagcaacctc aaccaggtgt ggaccagccc gccggaatac
      241 aattccccgg taaatggcga cgatctgccg atcgaccttg cggttaactc ctccggtgat
      301 gtttttgttg tcggtgcaag tgaagccgac accattgcag cgcgtgttaa ccggaattgg
      361 gtcattatcc gatacacacc agccggtacg caaagttggg tgacaaccgt cgacggtacc
      421 ggggccgatg atgatgaacc aaacgcagtg gcgttgcgtg gaacttcgtt gtgggtaacg
      481 ggttataccg tgggtgccgg aacacaaaag gacctgaccg tgaaccgcta tgatatttcc
      541 acaagcatca gtttccccga tcgtgtggac atcatgctgt ctgctttccc caaccccatg
      601 actgcatcgg caacactcga tttcgccaag ccgctgaccg ctggttcggt gctcgaagtc
      661 tcggatatca ccggcaaact ggtgcaacgc cgaagcgtca gcggaagccg cgtcctgatc
      721 gaacgcaatg agtgggattc cgggctctat ctggtttccg tcagcacacc ggatggcgac
      781 cgctatctgg ctaaattgat cgtacaataa cacctgccat gcgcgcaagc ttgctttcct
      841 tcatcctctt actgaccttg acaacagcag gcttcgcaca aagaaacctg agtatccggt
      901 cgactttgca gattcccgga caaaccctgg ccggttgttg gcactacgag gatggttcag
      961 gaaagatcta tgcattggta ggcgcggcac aggggatcat catcatagac attactgatc
     1021 cgtccagccc gcagcaactg tttcaacttc cgggagcaag cagcctttgg cacgaagtca
     1081 aagtgcaggg agattatgca tacgccgtca gcgaaggagt tgattcactt ggaatcaaga
     1141 acggtttgca gatcatcgat ttgcgctttc tgcccgatag tgcgcccaac aagttctggc
     1201 aaggcgacgg agcctgggcc ggacaattgt tgaccggtca tacggtcact accgcaggaa
     1261 actttgtgta cgtgaacggg cacaaccttt cttcgcttgg gcgcggtgta ttcatcgccg
     1321 acatcagtga tccctggaac ccaacgtatg tgggggctgt gaccagccgg tactgccacg
     1381 acagttatgt acgcggcgat acgatctgga ccagtgacat tatcgatggt ctgttttctg
     1441 tctacgatat caccaaccgg caaaatccca tcctgctcgc gactcagcag acacccggtc
     1501 aattcaacca caacagttgg ctgagtgatg acggacgcac acttttcact accgacgaac
     1561 gttcgaatga acccctggcc gcctttgatg tcagcgacct gagcaacatt tccctgttgg
     1621 atgagttcta caatgaaaac atgtcctccg cggaagttca taatatcagg gtaatcaacg
     1681 actacctgat caatccgagc tacggaagtc aactcaccat cgccgatgca tccgatccgg
     1741 agaacctcat tgaagttgcg aatttcccta ccggttcttc cctgtgttgg gacgcggatc
     1801 cttatctgag caatggattg atcattgcga cggatatgtc gtccggtatc ttctatgtct
     1861 ttgaaccgaa ctatgttcgt gctgcccgcc tggaaggaac cgtaaccgat agtgtgtccg
     1921 gactaccctt gagcgatgtg gcggtggaat tgctttcaac aacacgcccg gtgcgaaata
     1981 ccaatttgct tggaaagtat aagacgggca ttgtcgatgc aggtgcttat acggtgcggt
     2041 tcagtaaagc gggctaccag acaaaagatg tcaccggaat caatctggtg aacggttccc
     2101 tactcaccct ggatgttcaa ttggtttcct cttccatcgg tattgaagag catgaccaat
     2161 atgcggcttt ggttgcattc cccagtccgg tagatgagtt cctaaacatt cgacttgctt
     2221 ccaacgcgcc cgtggattgg atgatctcgg atagtcaggg gcgacttgtt tcgcaaggga
     2281 aaatggtgcc tgcttccagc tccctggtcc ttccggtgaa cgaactcgcg cagggagtct
     2341 attcgattcg cctgtcgcag tcgtctcagc tccgtcaagg gcgctttatc gtagctcgtt
     2401 gatcatagtc cggcagcgat ccggtcgagc accgtttcta tcaggcggtt catcgtctca
     2461 tcccctttgt gggtatgttc attggtgatc cggttggcca caatggcatt gatagagcaa
     2521 caatgatggc ccagtatcct ggccagtccg tacatggccc cggtttccat ctcgaagttg
     2581 gtgatccggt gttcgcccaa gcggaagctg tgcagcgttt caataaaatt cggccgggcc
     2641 aattcataac gcagcacacg accttgagga gcgtaaaatc cagagcatga agcagttatc
     2701 cctttaaaca tcccttctga aagcgccatc tctaatttgt tggagcagcg tgccaggtag
     2761 gattggggaa ggataccttc attggggtaa tgatgcctga aagcttccac gatttgctgt
     2821 tcctcctggt cgtttttcaa tcggtagaag ttgagcaatc cgtccagtcc cagaccaaac
     2881 ctggaaaaaa cgaatccgtc gaccgggatc tctttttgga gggagcctga ggtcccgatc
     2941 cggatgaggt tgagtgactt aagttgtggc tggatctcgc gctttcgtag atcgatgttg
     3001 accagggcgt ccagttcgtt gtagacaata tcgatattgt ctgtaccgat acccgtagat
     3061 aaaacggtga ttcgcttgct tttataggtt ccggtatgcg taacgaattc ccgtttctgt
     3121 ttacgtattt cgatcgaatc gaagaacttc gaaaccatac cgacccggtc aggatcaccc
     3181 acgttgatga ctgtatctgc gatttcttcc ggatgcaggt tgagatgata gacactgcca
     3241 tctgaattca aaatgagttc ggattcgggt attttgctca tctgagggtt tccgttattt
     3301 ttgctcacaa gataaaaatt cgtatggccc aaccgaagaa ccacattctg gttccgatcg
     3361 acttttccga acaatccctc atcgctctcg gtcaatccta caacctggcg cggctcaacc
     3421 gcgctgattt gacgctcatc catatcatcg acgaatcctt ccatcttccg tttatgggga
     3481 agaaagagga taagtcgatg gagaagaaga ttcagaagga attagataag ctcgcttcgg
     3541 aaacttcgca aactgcgggt atcaaggtga ataccatggt gacccgcggg aagatctacg
     3601 aagagatcca aaaagcggtc aagaaactca aggtttccct gatcatcatg ggaaccaatg
     3661 gcagtgtagg cttcaagcgc ttcatcgggt cgaacgccct tcgcgtcatt cgggaagcca
     3721 gttgtccggt gatcacgatc aaagggaaga agcaccgggc gggttgtaag catattgtgc
     3781 tgcccctgga tctgcaaaag gagaccaaag agaaagtcca caatgccatc gaattctcca
     3841 agctgttcgg ttcgaccatt gatctggttt cggtactgac gaccgacgac gagttcatcg
     3901 tcaataagct caaacgccag atgcatcagg tgcacgagta tatcctggag cagaacatcc
     3961 cctgtaccgt tgagttcctc cacggcgacg atgtggcgga agaggtagtg aagtacgcca
     4021 aacgcatcaa ggccgacatc atcatggtca tgacgcaaag tgagatggac tggtccgaca
     4081 tgttcatcag ttccgcgaca caggatatca tcaattcatc ggatattccg gtgctcagcg
     4141 ttcatccgaa ggaaaagaaa gacaccacgc ttacgccttt tgaatattaa cctgtagatc
     4201 actgccatgc attcaaaaaa cttccaaatc aaacggatcc tgattccgta cgatttttcc
     4261 gagaccgctg acctggcgct tgaacatgct gtattcatgg caaaattgca caaagcggat
     4321 atcgtgctgc tccacgtgat cgaaacctat tcgttcacgt ccgccatcag ccacgcgttc
     4381 agcaagtcgc aatcggagtt cgaatccaaa atcgagagtt cggcttccga taagctacat
     4441 cacgatagtg gcatgggtgt acagttccgg acggaaatgg gcaagatcta caagacgatc
     4501 atctccgtag cggaagaaac ggatgccgac ctgatcgtaa tgggaaccca tggcgtaagc
     4561 ggattccagg aattcctggt aggcaccaat acttaccggg tcgtggtcgg tgcgccatgt
     4621 cccgtcgttt ccgttcagac acatgcccgt aagatcggct tcaagaatat agtactgccg
     4681 attgacaatt ccccggcttc ccggcaaaag gtcaaacacg ccgttgagat cgcgcgtcat
     4741 tacaattcca tcgtccacat tctgggcatt atgaccatga gcgacaccga tatgcaacgt
     4801 cgtttcgaac tgaaggtcca tcaggttgca gattacctgg ccgaacatga aattccttat
     4861 acgacgaagg ttttcaaggg cgacaacacc gctcagatca ccatggatta tgccacccag
     4921 tgtagcgctg acctgattgt gatcatgacc gagcaggagg gaggcggatt gttcatggga
     4981 aattttgccc aacagatcgt caaccattcc aagattccgg tcatgagtat ccggccggat
     5041 gaaggcgatc cggataagat ctccatcggt tactgatcga aacatcctaa aacggaaagg
     5101 ccatcacgtc gtgatggcct ttctgtttat aggtgccgga tcagtttcca aaaacccgct
     5161 tcaaaagcgc ggtcactcgg gccgcggggt ctctccggat cttggtttct tcctgtgcga
     5221 ccagcgtaaa caaaccgctc agagcccgct gggtgatgta ttcttcgagg ttaggattga
     5281 cgggatctac gaacggtatc ttgttgtagg tggtcgcgag cggattccag taacgggtaa
     5341 cactgacctt ttcaatcgcg cgctgaatga tcggtttgaa tttggtgtga agtgcacctg
     5401 aggttttttg tctcaggtag cccgtagcgg ccgtatcagc accccgcagg atgttgaagc
     5461 cgtccgtaac ggtcatgccc gtgatcgcgt ccagaaagac cggtgcggcc tctttggcag
     5521 cctcctcagc ggcccggttg acggtcagga tgaagtcatc gacttgtttg ttcatcccaa
     5581 gcgaccggag cttggtttcc atggatttcg cttccggagg aaaagggatc ttgatcaggt
     5641 tattgccgaa gtagccatcg gttttggcgg cactacccgc ggcattgcgg cttcccacct
     5701 gtagggcttc cttcaatccg gctacgactt ccgattccga caggggttta ctaccctgaa
     5761 cggtctcgtt cacactcttt tcaagttttt taagatcgat ctgggaacag ccggccaacg
     5821 gaagggccag gaggaggagg gaaaggagtc ttcgcataaa ccggtttatc cggcgaagaa
     5881 acaaacttag taccaaagtc agtctgctct cctcaagatt cagtttcggc tggtggtgca
     5941 gggggaggtg ttgatttgcg gcagcgttgt ttccagaggc ttcgcacacg ttcacgttcc
     6001 tccgggctca tgccttccca acgttctttc caactgccat cgcgacaatg accaccaccg
     6061 cttttccagc ctttagcgct accaaagagg atcttactca gtacgaggat ccccaatgcc
     6121 tgccagaatc cgatcaccgg cccggtgaag agggcaggta tcagccaatt ccaaagggtc
     6181 atcactaccc aaccggccag tgccccgaat accacaaggc cgaccattat tttcattatc
     6241 catttcgctt tcatttctgt agtgattttt agggtgtgtt gagttcgtcg taatattcct
     6301 gtagccgctc ccgtagtttc agaatagcgt aacgcttgcg cgcgagaagg gtgttgatgg
     6361 aaaggccgga ttcatccgac aactgctgaa agctctttcc ttccagttcg tgcgcgatga
     6421 aaacctcccg ctgttccaca ggcaattcgt caagcgcgct ttcgatggct tgcagcaata
     6481 agcttctgtc gaattccgcg tcaggtcccg ccgtatcgct cctgagcaac tcatcgacca
     6541 gcagggtctc ttccccggat gggtcgggaa accgaaagta gtcctcgagg cgcacgcttt
     6601 ttttcctgcg gtagcggtca gtgatcttgt tgcgtgcaac ccggaacagc caggcggcca
     6661 ttttttcgat gggttgcatc agccggtatg cggacgtgag ttcaagcacc acatcctgaa
     6721 aaatgtcatc cgcatcttca ggttcagcga cacgggaacg gatgaagtcc cttagcctgc
     6781 gaccattctc gcgcaatgct gttcgtatgc tttcgtcctg ggactgcgtc atcgccgatc
     6841 gggatgtagg ggcaagttcc atataatccc gcagacgccg aaggatgccg gatattgcaa
     6901 acttccatga cttttttctg ttcttacctt tgacagcacc tatgctagcc gaaatcatca
     6961 ccatcggcga cgagctcctg atcgggcaag tcgtcgacac caacagtgcc tggatcggtc
     7021 aacgactgaa cgagatcggg gtccggatca aacagatcac ttctgtcagc gataacgagg
     7081 agcacatcct caaggcgctc gacgaggccg ggcagcggtc gggcatcatc ctgatcaccg
     7141 gtggtcttgg tccgaccaaa gacgatatca ccaaaaagac ccttgccaaa ttcttcaagg
     7201 cgggtatgcg tttggatggc ccttccctgg cgaatgtcga acgcatcttt cgggcccgtg
     7261 gaagagaagt gaccgaattg aaccgccgac aggcggaagt accggaaaac gctaccgcat
     7321 tgctcaatcc gctgggaacc gcccccggga tgtggttcga ggaacacggc aggatctatg
     7381 tctccctgcc aggagttccg catgagatga aaggcctgat ggagcaggaa gtgatcccgc
     7441 aactgcgcca acggttcaag cttcctcccg tcatccatcg gaccatcctt acccagggta
     7501 tcggcgagtc catgttgtct gatctgctgg aaccctggga ggatgcattg cctccacata
     7561 tccgtctggc ttacctcccg gcagatggtc gggtacgact ccgactgacc gcttcgggaa
     7621 agtcggaagc ggaaattaat gctgaattag atattcaggt agcaggacta aagaatttgg
     7681 caaacagata tatatatggc tatgaagaag aaaccatgga aggagttgtc ggcgccttat
     7741 tcagagccaa aggatggaaa gtcgccactg cagaaagctg tacaggcggg tacctgtcgc
     7801 atcgcttgac cctggttccc ggtagttcgg attattacat gggctctgtt attgcctacg
     7861 ataacgcggt aaaaacgagc ctgcttggtg ttgatcccaa gcttttcgag gaggtgggcg
     7921 ctgtcagcga accggttgta cgggtcatgg cggaaaatgt gaggaagctg ctgggaacgg
     7981 agttgggcat tgccatttcc ggaatagcag gcccaagcgg ggggacagaa gagaaaccgg
     8041 tcggaaccgt ctggatcgcc gtgagcagtc cggctgggac tttgtcgaag agatttcaat
     8101 taggccaatt ccgtgaacgg gtcatccggg aagcagccca acacgcgctc gccatgactc
     8161 gaaggatcct gttagcagaa cctgaaccgg aaaattgaat cttatataaa tatatgtaaa
     8221 tcaagcgttt gtaaataccg agcagttgat tgaagtctcg gcgattgtga aaaaaacctg
     8281 ttgaaaacgc gctgaaaatc cacctttcat tttgcaataa tcccaaagaa gtgtatcttt
     8341 gcactcctta aaaattcaac tatcatggca cggatttgtg atctgaccgg aaagcgcgcg
     8401 atgggcggcc accatgtttc cttctccaac aagaagtcga agcgtaaatt caacccgaac
     8461 ctgcaagtga agcgtttcta cgtgccggaa atgggtgagt ggatcacctt aaaagtttct
     8521 acttccgcac tgcgtaccat cgataagatg ggcatcggtc cggcaatcga aaaagctatc
     8581 aagaagggta agatctgatt cttactatcc tgtgaggaag tgctaaattc gggaacgggt
     8641 ttagcacttt cgcttttata cccagtccat catgcgcgtt gtcagtatcg tgttatggct
     8701 gttaatcggt ttgccggtag ccagtttgtt ggcacaggat tcggaccgct accggaatga
     8761 gcaggacttc ctcacgcctg aattttacaa ggctcgtcgg gaagccttgc gtcaacttat
     8821 cccggagggc tcctgtgctg tcctgctgtc ggctcctgtg aggaaccgat cgaacgatgt
     8881 ggattacccg tatcatcaaa gtcctgattt ctattacctc agcggctgga cagaacccaa
     8941 ttcagtgctg gtggtcttta aggaagccca acgattcaac aaaggtgagg taaaggatat
     9001 cctgttggtg cctccacgcg acccggcgaa agagcgatgg aacggcaggc gtggtggtaa
     9061 gacggccgcg cgcaggatca gcggtgctga ctgggtgttg acgaccgaga gttgggacac
     9121 cttgcaactg ccgtttgaat cgcaaacgaa ggtattgcag ctccggctgg aagatccccg
     9181 catcgccggg aatcaggatc ccttggagta cgatggtctg ttgcggtcgt atcggaagaa
     9241 ggtcgacagc gcctccgtgt ctccggatga tttcctgttg aagcgggcac tcaccaccat
     9301 gcgaatggtg aagcatccgg atgagttggc cttgttgcag cgggccgtgg atatcagtgt
     9361 cagcggacac ttggaaatga tgaaagcact ggaaccagtt atgacggagt atcaggtgga
     9421 agctgtgggc gaatatgttt ttcatgcctt gggtgcagaa gcggtgggat atccaagcat
     9481 ctgcggtggg ggtgaaaact caaccgttct gcactacacc gacaaccgac gcccgctcgg
     9541 agccggtgac ctgatcctac tcgatatggg tgcggaatac cacggctata cagccgatat
     9601 tacccgaacc cttccggtca ccgggaagta cacgcccgag caattagcca tctataacct
     9661 cgtactcgaa gcgcgtgatt ccgggatagc tgcttgtcag cccggaaatc cctttcaagc
     9721 acccaatgcc gctgcccgca gggtgatcag ccggggtttg caagatttgg gaatcatcca
     9781 atccgcggat gagctcaaga cctatttcat gcacggaact tcgcattacc tgggtctgga
     9841 cgttcacgat gcaggtaatt acgggccatt ggcaccgggt aatgtcatta ccgtcgaacc
     9901 gggcatttat atagctgcag acagtccatg cgatcgcaaa tggtggggaa ttggcatccg
     9961 catcgaggac gatatcctga ttacaacaac cgggcatcag aatttgagcg aaaaactgcc
    10021 cgtaaagccc gaagagatcg aaaaagccat gaaacagcct gccattttga acaattcagc
    10081 gctcaaaaat gaatagaaag ccttgattag atagggaata ttcgtatctt tgcagccctt
    10141 ccggggaaat tcccgggatt ttcaatcaat cgtcgtcatg gcaaaaaaag gcaatcgcgt
    10201 tcaggtgatc ctcgagtgca ccgagcacaa gaccagtggt cagcccggca cttcgcgcta
    10261 tatcaccacc aagaacaaga agaacaccac cgagcgtctg gagttgaaaa aattcaaccc
    10321 catcctgaag aagatgacgg tgcacaaaga gatcaagtaa tcgttaacta cgaataaccc
    10381 agtaccatgg caaagaaagt agtggcaacc ctgaagaccg gtaagggaaa ggaattctca
    10441 aaggtcatca agatggtgaa gaacccggat accggttctt accaattcaa ggaagaagtc
    10501 gtgcacaacg accacatcaa ggatttcctg aaagagaaat aagccctgtc agacaacatc
    10561 gacaaagctt ctttcgcatg aaggaagctt ttttaatttc cataccccag tcccttccgc
    10621 ctcatgggtc tcttcagttt cttttccaaa gagaaaaagg aaagtctcga caaaggactc
    10681 gagaaaacca aaaccagcct cttttccagg atcgccaaag ccgtcgttgg caaatcgacg
    10741 gtggacgcgg aagtgctgga caacctggaa gaaatcctcg tttcttccga cgtcggtgtg
    10801 aacaccacgc tacgcatcat caaacggctt gaggagcgcg tcgcgcgcga taaatacgtc
    10861 ggtacggagg aactcaaccg cctgctcaaa gaggagatca ccggactcat ggcggagaac
    10921 aataccgccg atgccacgga attctcgctg cccgataccg gcggacaacc ctacgtcatc
    10981 atggtagtcg gagtgaacgg tgtggggaaa accacgacca tcggaaaact ggcctatcat
    11041 ttcaagaaag cgggcaagaa agtggtgttg ggcgccgcgg acaccttccg tgccgctgcc
    11101 gtcgatcaac tcgacatctg gtctaagcgg gtggatgtcc cgatcgtcaa acaggggatg
    11161 ggctcggacc cggctgccgt cgcgtatgat tccgttcagt cgggtgtggc gcaagaggcg
    11221 gatgtcgtga tcgtagatac cgccggtcgt tt
//
LOCUS       DAOTON010000004        10794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_5731_length_10794_cov_4.057827, whole genome shotgun sequence.
ACCESSION   DAOTON010000004 DAOTON010000000
VERSION     DAOTON010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10794
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5731_length_10794_cov_4.057827"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="P5292_00180"
     CDS             <1..188
                     /locus_tag="P5292_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRS37569.1"
                     /translation="WVNVARNAAPRASIAIKADRDADLPTIKRGISILQDNKANRFAL
                     ITNLEGAPTAEPATDKQ"
     gene            233..877
                     /locus_tag="P5292_00185"
     CDS             233..877
                     /locus_tag="P5292_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRS37570.1"
                     /translation="MAEVQQQEGGSDKGKHKKVRAKKSSTHIDMTPMVDLAFLLLTFF
                     ILTTTFSKPKTMDITMPVKDKILDEERTKVPASQTLSLLLTENDRIIWYIGEDDPAKP
                     PSTNIADYSQDGAKSIRKILLEKNKLVIDQVKLVEDSVKAGLIPDTDEDIKRHKSAVK
                     AAEKKGLIVLIKPDDKSKYKNLVDILDEMLVCNVGRYAIVDLSDSEKTLIENTK"
     gene            889..1686
                     /locus_tag="P5292_00190"
     CDS             889..1686
                     /locus_tag="P5292_00190"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRS37571.1"
                     /translation="MKLFSWDNAVNPDRVELVFEHRNKEFGAYVLRSKYQRRILTAML
                     ISFVAAIVFFLLPQLMTLLSSVKFNDKSTVTEVVTLAEPPPIDKSTPPPPPVIPPPPV
                     QQTIKFTPPKVVKDEEAQEPPPTQEEVKEVQVSTVTQEGSKDVVDLPPDNPVVGDPDE
                     GKIFTVVEEMPSFPGGEEELFKYLQRNIKYPAIARENGISGRVYVTFVVDKDGKIKDA
                     KILRGIGGGCDEEALRVVRNMPEWKSGRQNGRPVQVQYNLPVNFTLK"
     gene            1701..1931
                     /locus_tag="P5292_00195"
     CDS             1701..1931
                     /locus_tag="P5292_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37572.1"
                     /translation="MADESSRPASRFRFYLNATMAVLYSAAGIVVFFFWTTSQVPANN
                     RRIAGGALMIYGVYRFYRSLRAYRAETTPQQP"
     gene            1928..2869
                     /locus_tag="P5292_00200"
     CDS             1928..2869
                     /locus_tag="P5292_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRS37573.1"
                     /translation="MNTRRLRLLYLLPLFWLFACGNRTDRPSYTDTPTSGTIDIVVDE
                     SYEPLIKVQLDTFMRIYKYANIRARYLPEAEVMNELLHNDSVRIAITARKLTKDETTE
                     FDKQRIIPRYTKIAVDAVALLVHPDNPDTALGTEQLRAIVQGKTSTWKMLDPKKGSAD
                     TIRIVFDRSGSSNTRYLKEEFLEGGNFPPNCYATNSNADVIDYVATHPNALGIISVNW
                     ISDSDDPTVNEFLKKVRVVYLAAEGESAFVQPYQAYIALKSYPLTRDVYVISREGRNG
                     LGTGFAAFAAADQGQRLVRMMGLLPATMPVRIVNLHE"
     gene            2878..4554
                     /locus_tag="P5292_00205"
     CDS             2878..4554
                     /locus_tag="P5292_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS37574.1"
                     /translation="MKNRFRYLLAALLLPGFVAAQSLEEARKLTENEQYDAATNVYQQ
                     LISREPANGNLYYFFGDNYLLGDNADSAMIVFEQGRKADAANLLLNIGMAKSLMDRMS
                     VREAKSAAEKDPSNAEAAARYADAQRRVAEAQQLIDDAVAKAPAKSAAVLIEAAEALI
                     HYTNKDLDKAKTLLDRALTIEPKNPEIHLLLGDIYTELNNGTLAADYYNKALDYNKTS
                     ARGIVSKGRLYKRSTNYEGAAAEFEQAIQLDPNYAPAYRELGETYFRLGRLEKAKEQY
                     RKYLELSRNNCNARIRYASFLYLSKEYTNALNEINQVKQKCDSGNVTLLRVESYCYFE
                     IKDYQRGLFVCNRLFDKLLEDKRTVTDYEYYGKILIGSGRDSLGVENLRRAYGMDPSR
                     VDLLSELANAYMKMKRYPDAIQVLNEKITLGKDVKVADYFNLGRSYYFNHQFAPADSA
                     MMKVNELSPKYASGYLWRAKANTQIDSTSELGLAKPHYEKYIELAEADSANAAKYNSG
                     LIEAYGYLAYYYILKKDNANALLYLRKKAALPLEPDDKKNVEDAIRQLSK"
     gene            complement(4625..6979)
                     /gene="rnr"
                     /locus_tag="P5292_00210"
     CDS             complement(4625..6979)
                     /gene="rnr"
                     /locus_tag="P5292_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRS37575.1"
                     /translation="MSKKKRKDENPVEHFILSELYKAFNQHPNRSFNHKQLARHLREP
                     YFDFIDRLPDSPEQNREEQGEELRNEIIRLLGDLAFKGELIEVERGKFKLKPKHSYLE
                     GIIDITASGAAFLLSENDEDDVFIAPRNVRQALHGDRVKVFLYAKHRNQRIEGEVVEV
                     LERARTEFVGTVQLVGKYAFVVPDSPKMLIDIFIPGHLLSNAEQGQKVMAEIVDWPKN
                     AKNPMGRISRVLGWPGENDAEMHSILAEYGFPLEFPAHVEKESEAISEEITPQEIRKR
                     RDFRSVPTFTIDPIDAKDFDDALSIRPAGEGRWEIGIHIADVTHYIREHTRLDKEAYE
                     RATSVYLVDRTIPMLPEKLSNGVCSLRPGEDKLCFSVVVVMDEHAKVHDTWFGKTVIF
                     SDKRFHYDEAQAVIETGQGPFSEEIGLMHRLAQRLRTERFRKGAIAFEKVEVKFQLDE
                     QGRPIGVYLKESKDANKLIEEFMLLANRSVAEFVGKRHRVTGQGTKKGKHQEEVEHLT
                     VEAKPFVYRVHEGPVNERLQSFAAFAGRWGYQIKTGNDREIAHSLNDLMKSVRGKKEQ
                     NVLEQLAIRSMSKAVYTTDNVGHYGLAFDYYTHFTSPIRRYPDVLVHRLLERYLASGQ
                     DVDVEALEEQCKHCTAMEIKASDAERTSIKYKQVEFLQDKLGQTFDGIISGVTEWGLY
                     VELSENKCEGLVRLRELEDDFYEFDDTNLRVIGSRTGRVYSLGDAVRVTVLKADLQRR
                     QIDFRMERNPEHHTPLMEAEARRSSRERPGRNGFRAPKKAKKRR"
     gene            7178..8077
                     /locus_tag="P5292_00215"
     CDS             7178..8077
                     /locus_tag="P5292_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37576.1"
                     /translation="METQNQDQKKGKGTLWLVLLILSGALNLYQWQNHSTTVNTYEQK
                     VDTLVVERVNVEKELAETKSELEKYRGISANLDSLLNEANAKLEEQAKKIKSISSQKQ
                     SMTELNEKLKKQLADLQMLRDEYLGRIDSLLMANKQLQTEKEQLTSNVETLSKNLETT
                     VATASVLKAEYIKVTSYKRKDSGKYVATLLAKRTHKMDVCFDVLDNKIAKQGDKTVYL
                     KITEPGGKPVGNRSTGSGTFKTSTGEEVMYAATGTLNYSGSKQNICLSYEEQEDKKFP
                     AGTYLIEVYVDGNVAGAGSYTLR"
     gene            complement(8153..8440)
                     /locus_tag="P5292_00220"
     CDS             complement(8153..8440)
                     /locus_tag="P5292_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRS37577.1"
                     /translation="MGDKTEPLLLLDFQATWCEPCKWSEPVMEQVLRHFGDRIVLEKI
                     DIDQFPERAREFHVLSVPTFVLLRDGKEIWRYRGFDSAPAMIRMMEPLLSV"
     gene            8521..9486
                     /locus_tag="P5292_00225"
     CDS             8521..9486
                     /locus_tag="P5292_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRS37578.1"
                     /translation="MMSVTAGTGGSPRNTRTVIGMVLFLAFLVAIPALGWSPSIALVC
                     GLLVGIIFGNPFPQYTKPVTKYLLQASIVGLGFGININKVVAAGQEGFVFTLLSLSAA
                     IGLGYLLGRLFRVDRVISYLVSVGTAICGGSAIAAVSQVVEADDQEVSISIGTVFILN
                     AIALLIFPPLGHWAELTQHQFGIWAAIAIHDTSSVVGAAASYGNEALTVATTVKLARA
                     LWIVPLALLSAAIFKKKSSWGGFPWFILFFLVASMLGSWVNMPLILLAAIVHSAKAGF
                     SITLFLIGAGISLGALKRVGPRPLLLGVVLWVIILVSSLYLISRY"
     gene            9539..>10794
                     /locus_tag="P5292_00230"
     CDS             9539..>10794
                     /locus_tag="P5292_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRS37579.1"
                     /translation="MQIAIVLGLLFVALILFSWDRIRIDVVTIGLLAILMAAGILTPM
                     EAFAGFGSDFIIMLAAIFVISGALQQTGVLDLVGSRMLKIAQLRPAALLTLVMFSVGF
                     TSAFMNNTTVTAIYIAPVMAVARKLKMSPSRLLLPVAYASILGGCCTLIGTSTNIAVG
                     GYLHRNGIGEIGMFDLLPVGASMFVVGWIYMNTIGKRLLPDRPEVSVTDEFGLREYVS
                     EVVITPGSPLIGQTVFHSNLSRMGFRILNMVRGEDRFIPDPTTRILEHDLLIVEGKIN
                     DLLEVREREGISIRADLLIDRELQSERFKLAEILVTPESEFLNSTLKEAKFRQRYGLT
                     VLAINRTGYSVNEKLGRFVLRSGDVLLVQGSVETISYHKRSKRMQVMSDFMPLLYRKS
                     KGLLTLGLFVAAVIIGSLGWLPLSVT"
BASE COUNT         2410 a         3083 c         2992 g         2309 t
ORIGIN      
        1 tgtgggtgaa tgtcgcccgt aacgccgctc cgcgtgccag catcgcgatc aaagccgacc
       61 gcgatgccga ccttccgacc atcaagcggg gcatctcgat cctgcaggat aataaggcga
      121 accgtttcgc cctgatcact aacctcgaag gcgcacccac ggcggagccc gccaccgata
      181 aacagtaaac cgctgcgacg ggcaaccgtc gcgcaagccg ggaacacgaa ccatggcaga
      241 agtacaacag caagaaggcg gtagcgacaa gggcaaacac aaaaaggtcc gcgccaagaa
      301 atcgtccacg cacatcgaca tgacgccgat ggtggacttg gcgttcctgc tgctcacctt
      361 cttcatcctc accacgacct tcagcaaacc gaagacgatg gatatcacca tgccggtgaa
      421 ggataagatc ttagacgagg agcgcaccaa agtccccgcc tcgcaaaccc tttcgcttct
      481 ccttaccgag aacgatcgca tcatctggta catcggggaa gacgatcccg ccaaaccacc
      541 gtccaccaac atcgccgact actcccagga cggagcgaag agcatccgca agattctcct
      601 cgaaaagaac aagctggtga tcgaccaggt gaagctggtg gaagactccg tcaaggccgg
      661 tttgattccg gacaccgacg aagacatcaa gcgacacaag tcggctgtga aagcggccga
      721 aaagaaaggc ctgatcgtgc tgatcaagcc ggacgataag tcgaagtaca agaacctggt
      781 cgacatcctc gacgagatgc tggtctgcaa cgttggccgt tacgccatcg tggacctctc
      841 ggactcggag aagaccctca ttgaaaacac gaaataaccg cccgaagcat gaagttgttc
      901 tcctgggata acgcggtgaa ccccgatcgg gtcgaactcg tgttcgaaca ccggaataag
      961 gaattcggag cctacgttct ccgcagtaaa taccagcgtc gcatcctgac cgcgatgctg
     1021 atctcgttcg tagccgcgat cgtcttcttc ctgctcccgc agttgatgac actactctcg
     1081 agcgtgaagt tcaacgacaa gtcgacggtg accgaagtgg tcacgctggc cgagccgcct
     1141 ccgatcgaca aatcgacgcc tccacctccg ccggtgatcc cgcctccgcc ggtgcaacag
     1201 accatcaagt tcacgccgcc gaaggttgtg aaggatgaag aagcacagga acctcctccc
     1261 acccaggagg aagtaaagga agtacaggta tccaccgtta cgcaggaagg gtcgaaagat
     1321 gttgttgatc ttcctccgga caatcccgtt gtgggcgatc cggatgaggg taagatcttc
     1381 accgtggtgg aagagatgcc gagttttccg ggcggagaag aagaactgtt caagtatctc
     1441 cagcgcaaca tcaaatatcc ggcgatcgca cgcgagaacg gcatttccgg tcgcgtctat
     1501 gtgaccttcg tggtcgataa ggacggcaag atcaaggacg ccaagatcct gcgcggcatc
     1561 ggcggaggtt gcgacgaaga agcgctgcgt gtcgtgcgca acatgcccga atggaaatcc
     1621 ggtcgtcaga atggtcgccc ggtgcaggtg cagtacaacc tgccggtaaa ctttacgctt
     1681 aaataagaca acccacacgc atggcggatg aatcgtcccg gcctgcgtcc cgtttccggt
     1741 tttacctcaa cgcgacgatg gccgtcctgt atagcgcagc gggcatcgtc gttttctttt
     1801 tctggacgac gagccaggtg ccggcaaaca accggcgaat cgccggcggc gcgctgatga
     1861 tctacggcgt gtaccggttc taccgaagct tgcgcgccta tcgggccgaa acaaccccgc
     1921 aacaaccatg aacacccggc gactacgatt gctttacctg cttccgttgt tttggctgtt
     1981 cgcctgcggc aaccggaccg accgaccgtc gtacaccgat acacccactt ccggtacgat
     2041 cgacatcgtc gtggatgaat cgtacgagcc gttgatcaag gtgcagcttg acaccttcat
     2101 gcgcatttac aagtacgcga acatccgggc gcgttatctg ccggaagcgg aggtgatgaa
     2161 cgaattgctc cacaacgatt ccgtccgcat cgcgatcacc gcacgaaaac tgaccaagga
     2221 cgaaaccacg gagttcgaca agcagcgcat catcccgcgc tacaccaaga tcgccgtcga
     2281 cgcggttgcg cttctggtac atcccgacaa tcccgacacc gccttgggga ccgagcaact
     2341 gcgtgccatc gtgcaaggca aaaccagtac ctggaagatg ctggatccga aaaaagggag
     2401 cgccgacacc atccgcatcg tatttgatcg cagtggttcc tcgaatacac gctacctgaa
     2461 ggaggagttt ctcgaaggcg gcaactttcc gccgaactgc tacgcgacga attcgaacgc
     2521 ggacgtgatc gattacgtcg ccacgcatcc gaatgccctg ggcatcatca gcgtgaactg
     2581 gatcagcgac tccgatgatc ctacggtgaa cgaattcctg aagaaagtac gcgttgttta
     2641 cctggctgcc gaaggcgaaa gtgcctttgt tcagccatat caggcgtata ttgcactgaa
     2701 atcgtatccc ttgacgcggg acgtctatgt gatcagccgg gagggccgaa acgggcttgg
     2761 cacgggcttt gccgcctttg ccgccgccga tcagggccag cgcctggtgc gcatgatggg
     2821 cctgctgccc gctaccatgc cggtccggat cgtcaaccta cacgagtgaa aagaaccatg
     2881 aagaaccgat tccgttacct gctcgccgca ctgctgctgc cgggctttgt cgcggcgcaa
     2941 tcgctggaag aagccaggaa gctgaccgaa aacgagcagt acgatgccgc gacgaatgtt
     3001 taccagcaac tcatctcgcg cgaacccgcc aacgggaacc tgtattattt ctttggcgac
     3061 aactatctcc tcggcgacaa tgccgattcc gccatgatcg tatttgagca gggacgcaag
     3121 gcggatgccg ccaacctgtt gctcaacatc ggaatggcca agtcgctgat ggatcgcatg
     3181 agtgttcgcg aagccaagag cgctgcggaa aaagacccct ccaatgcgga agccgcggcc
     3241 cgttatgccg acgcgcagcg ccgcgtagcc gaggcccagc aactgatcga cgacgcggta
     3301 gccaaagcgc ctgccaagag cgcagcggtg ctgatcgaag ccgcagaagc cctgatccac
     3361 tatacgaaca aggaccttga caaggctaag acccttctgg accgcgcgct gaccatcgag
     3421 ccgaagaacc cggagatcca cctcctgctg ggcgacattt atacagagct taacaacggc
     3481 accctcgccg ccgactacta caacaaggcg cttgattaca acaagacctc cgcacgcggg
     3541 atcgtgagca aggggcgctt gtacaaacgt tccaccaact acgaaggcgc ggccgccgaa
     3601 ttcgagcaag ccattcagct ggatcccaac tacgcccccg cttatcgtga gctcggtgaa
     3661 acctacttcc gccttggtcg tctggagaaa gccaaggaac aataccgcaa gtacctcgaa
     3721 ctctcccgca acaactgcaa cgcgcgtatc cggtacgcgt ccttcctcta cctgagcaag
     3781 gagtacacga acgccctgaa cgagatcaac caggtcaagc agaaatgcga ttcgggtaac
     3841 gtcaccctgc tgcgtgtaga atcctattgc tactttgaaa tcaaggacta ccagcgcgga
     3901 ctcttcgtgt gcaaccgact gttcgacaaa ctgcttgaag acaagcgcac ggtcaccgac
     3961 tacgaatact atggcaagat cctgatcggt tcaggacggg actcgctggg tgtcgagaac
     4021 cttcgccgcg cttacggcat ggatccgtcg cgcgtcgacc tgctttccga gctcgccaat
     4081 gcgtacatga agatgaagcg ctaccccgac gcgatccagg tgctgaacga aaagatcacc
     4141 ttgggcaagg acgtgaaggt ggccgactac ttcaacctcg gccgctccta ttatttcaat
     4201 caccagtttg cacccgcgga ttccgcgatg atgaaggtga acgagctttc gccgaagtat
     4261 gccagcggtt acctctggcg cgccaaggcc aatacgcaaa tcgacagcac cagcgaactg
     4321 ggcctggcca agccgcatta cgagaaatac atcgagctgg ccgaagcgga ctccgccaac
     4381 gccgcgaaat acaactccgg actgatcgaa gcctacggct acctcgccta ctactatatc
     4441 ctcaagaaag acaacgccaa tgccttgctc tacctgcgca agaaagcggc gctaccgctg
     4501 gaacccgacg acaagaagaa cgtcgaggac gccatccggc agttgagcaa gtaagcacaa
     4561 acgtccaaca gaaaacccgg cagcctacat caggctgccg ggttttttca tgggtcggat
     4621 tttctcaacg acgtttcttc gccttcttcg gggcccggaa accattgcgg ccgggccttt
     4681 cgcgggagct gcggcgcgct tccgcttcca tgagcggcgt gtggtgttcc ggattgcgct
     4741 ccatacgaaa atcgatctga cgtcgttgca gatcggcttt taacaccgtg acgcgcacag
     4801 catcgccaag cgaatacacg cggccggtcc gggacccgat cacgcggagg ttggtgtcgt
     4861 cgaattcgta gaaatcgtct tcgagttcgc ggaggcgcac cagcccctcg catttgtttt
     4921 cagagagctc cacgtagagg ccccattcgg tcacgccgga gatgatgcca tcgaacgttt
     4981 gtccgagttt atcctgcagg aactcgacct gcttgtattt gatcgaggtg cgttccgcat
     5041 cgcttgcctt gatctccatc gccgtgcagt gcttgcattg ctcttcgagc gcttccacat
     5101 cgacatcctg tccggaagcg aggtagcgtt cgagcagacg gtgcacgagc acgtcgggat
     5161 aacggcggat gggggaggtg aagtgcgtgt aataatcgaa tgcaagccca tagtgcccga
     5221 cgttatcggt ggtatagacc gccttcgaca tactgcggat ggcgagttgc tcgagtacgt
     5281 tttgttcttt ttttccgcgc acgctcttca tcaggtcgtt gagcgaatgg gcgatctcac
     5341 ggtcatttcc ggtcttgatc tggtagcccc agcgtcccgc gaaagcggca aacgactgca
     5401 gccgttcatt cacgggacct tcgtgtaccc ggtagacgaa gggcttcgct tcaaccgtaa
     5461 ggtgttccac ctcttcctgg tgctttcctt ttttcgtccc ctgcccggtc acgcgatgac
     5521 gttttccgac aaactcggca accgaacgat tggcgagcag catgaactct tcgatgagct
     5581 tgtttgcgtc cttactttcc ttcaggtaga cgccgatggg gcgaccctgt tcgtcgagtt
     5641 gaaatttgac ctccaccttt tcgaacgcga ttgcgccctt gcgaaagcgc tccgtgcgga
     5701 ggcgttgggc cagccggtgc atgaggccga tctcctcgga aaaggggcct tgtccggtct
     5761 cgatcaccgc ctgcgcttcg tcgtagtgga aacgcttgtc ggagaagatg accgttttcc
     5821 caaaccaggt gtcgtgcacc ttggcgtgtt cgtccatgac gacgacgacg gagaaacaca
     5881 gtttgtcctc tcccgggcgg agggaacaga caccgttcga cagtttttcg ggcagcatgg
     5941 ggatggtgcg gtcgaccagg taaaccgagg tggcccgttc gtaggcttct ttatcgagcc
     6001 gggtatgttc gcggatgtaa tgggtgacat cggcgatgtg gataccgatc tcccagcgac
     6061 cttctccggc cggccggatc gataaggcgt cgtcgaaatc cttcgcgtcg atcggatcga
     6121 tggtaaaggt cgggacggag cggaagtcgc gccgcttgcg gatctcctgg ggggtgatct
     6181 cctcggaaat cgcttcggat tccttttcca cgtgcgcggg aaactccagt gggaatccgt
     6241 actcggccag gatggaatgc atctcggcgt cgttctcacc cggccagccc aggacgcggg
     6301 agatgcggcc catcgggttc ttggcgttct tgggccaatc gacgatctcc gccatcacct
     6361 tctgtccctg ctcggcattc gacagcaggt gtccgggaat gaaaatgtcg atcagcatct
     6421 tggggctgtc gggaaccacg aacgcgtact tgccgacgag ctgtacggtt ccgacgaatt
     6481 cggtgcgcgc gcgttcgagc acctctacca cttctccctc gatgcgctgg ttgcggtgtt
     6541 tcgcatagag gaacaccttc acccggtcgc cgtgcagcgc ctgccggacg ttccgtgggg
     6601 cgatgaagac atcgtcttcg tcattttcgc tcagcaggaa tgccgcgccg gaggcggtaa
     6661 tgtcgatgat gccttcgagg taggaatgct tcggtttcaa tttgaacttc ccgcgctcta
     6721 cttcgatcaa ctcgcctttg aacgccaggt cacccaggag gcggatgatt tcgttgcgta
     6781 gttcttcccc ttgttcttcc cggttttgtt cgggcgaatc cgggaggcgg tcgataaaat
     6841 caaaataggg ttcacgcaag tggcgcgcga gttgcttgtg gttgaaactc ctgttcgggt
     6901 gttggttgaa tgccttatac agctccgaca ggatgaaatg ttcaaccgga ttttcgtctt
     6961 tcctcttctt ctttgacatg tgttgcttga agtccgcgcg ttcagaaatg gcagcgactt
     7021 gacgcccgtc ttcttcacac aagatacaac ttcattcaga gaacaaactc actgtgcgca
     7081 atgtgaaaaa cacgtttcat aacttttagc atgactacaa tacccggttc ccactgccgt
     7141 tacttttatg aaacatttaa gcaccactta agcgaacatg gaaacgcaaa accaagatca
     7201 aaagaaaggc aaaggcacgc tatggctcgt gttgttgatc ctttccggag cgttgaacct
     7261 ttaccaatgg cagaatcact ccacgaccgt gaacacctac gaacagaagg tggatacgct
     7321 ggtcgtcgag cgcgtcaacg ttgaaaagga actcgccgaa acgaaatccg aactcgagaa
     7381 gtaccgcggt atttccgcga acctcgacag cctcctgaac gaggccaacg cgaaactcga
     7441 agagcaggcc aagaagatca agagcatcag ctcgcagaag caaagcatga cggagctcaa
     7501 cgagaagctc aagaagcagc ttgctgacct gcaaatgctc cgtgacgaat acctgggccg
     7561 catcgacagt ctcctgatgg ccaacaagca gttgcagacc gagaaggagc agctcacctc
     7621 gaatgtggag accctctcga agaacctcga gacgaccgtc gcgaccgctt ccgtactgaa
     7681 ggccgagtac atcaaggtca cctcgtacaa gcgtaaggac agcggcaaat acgtcgctac
     7741 cctgctcgcg aagcgtacgc acaagatgga cgtctgcttc gacgtgctgg acaacaagat
     7801 cgcgaagcag ggtgacaaga ccgtctacct gaagatcacc gagccgggtg gtaagccggt
     7861 cggcaaccgc agcacgggat cgggcacgtt caagacctcc accggcgaag aagtcatgta
     7921 cgccgctacg ggcacgctca actattccgg ctccaagcag aacatctgtc tgagctacga
     7981 agagcaggaa gacaagaaat tcccggcagg cacctacctg atcgaagtgt atgtcgacgg
     8041 taacgttgca ggtgcgggtt cctatacgct ccgttaagcg tccgcccaat caatagaacg
     8101 aagcgacgcg atcaatcgat cgcgtcgctt cgtttttatc aggcccgagg aatcaaaccg
     8161 acaggagcgg ctccatcatc cggatcatag cgggcgccga atcaaatccc cggtatcgcc
     8221 agatctcttt tccgtcccgc aagaggacga aggtgggcac gctcagcacg tgaaattcgc
     8281 gtgctctttc agggaattga tcaatgtcga ttttttccaa gacgatccga tcaccgaaat
     8341 gtcgcaacac ctgttccatc acgggctccg accatttgca gggttcgcac caggtagcct
     8401 gaaaatccag cagtagaaga ggttcagtct tgtctcccat gatgtaaaaa tacacatcgg
     8461 ccgtttgatc cttaggcgga agcggtaata accttgcgtc tgtttccgtc gtacttttgt
     8521 atgatgtcag taacagccgg aacgggcggc agccccagga acacccgcac ggtcatcgga
     8581 atggtcttgt ttctggcttt tctggtagcc ataccggcat tgggatggtc gccttcgatc
     8641 gcattggtgt gcggactgct ggtcgggatc attttcggca atccgtttcc ccaatacacc
     8701 aagccggtca ccaagtatct gttacaggca tccattgtcg gactgggctt cggcatcaac
     8761 atcaacaaag ttgtcgcggc aggtcaggag ggatttgttt tcaccctgtt gtcgctttcg
     8821 gctgcgatcg gtttgggcta cctgctcggg cggctgtttc gggtggaccg tgtgatcagt
     8881 tacctggtgt cggttggcac cgcgatctgt ggaggaagcg cgattgccgc ggtgagtcag
     8941 gttgtggagg ccgatgatca ggaggtttcc atttccattg gaactgtttt tatcctgaat
     9001 gctattgcct tgctgatctt tccaccgctc ggacattggg cggagctgac gcagcatcaa
     9061 ttcggcatct gggccgcgat cgccattcac gacaccagtt cggtggtcgg cgcggccgcc
     9121 tcttacggca acgaagccct gacggtcgcc acgacggtga agttggcgcg tgcgttgtgg
     9181 atcgttccgc tcgcgttgct atcggctgcg attttcaaga agaaaagttc gtggggtgga
     9241 tttccctggt tcatcttgtt ttttcttgtc gcctccatgc tgggttcctg ggtgaacatg
     9301 ccgctgatcc tgctggccgc gatcgtacac agcgcaaaag cgggattcag catcaccctt
     9361 ttcctgatcg gcgccggaat ttccctgggt gctctgaagc gcgtaggtcc acgcccgttg
     9421 ttgctcggcg ttgtattgtg ggtgatcatc ctggtctcgt cgctctatct tatcagcagg
     9481 tactaactac tgaatcgcag gtaacacgcg attgaatttt actaacttgt aagcgatcat
     9541 gcagatcgcc atcgtactcg gattgttgtt tgtcgcgctg atcctttttt cctgggatcg
     9601 gatacggatc gatgtcgtca cgatcggact gcttgccatc ctgatggctg ccggcatcct
     9661 gacaccgatg gaagccttcg cgggattcgg cagcgacttc atcatcatgc tggccgcgat
     9721 cttcgtgatc agcggcgcct tgcagcaaac gggcgtgctc gatctggtcg gttcgcgaat
     9781 gctaaagatt gcccagctac gtccggcagc tttgttgacg ctcgtgatgt tttccgttgg
     9841 atttacgtcg gctttcatga acaatacgac cgtcacggcg atctacatcg caccggtcat
     9901 ggcggttgcg cgtaagttga agatgagtcc gtcgcgattg ctgctaccag tggcgtatgc
     9961 ttccattctc ggcggatgct gcaccctgat cggaacatct accaatatcg cggtaggcgg
    10021 ctacctgcac cgtaacggga tcggcgagat cggcatgttc gacctgttgc cggtaggcgc
    10081 ttcgatgttt gttgtcggct ggatctacat gaatacgatc ggaaagcgat tgctgccgga
    10141 ccgcccggaa gtctcggtca ccgacgagtt cggccttcgg gagtatgtca gcgaagttgt
    10201 gatcaccccc ggttcgccat tgatcggcca gacggttttc cattcgaatc tcagccggat
    10261 gggatttcgc attctcaaca tggtccgggg agaggatcgt ttcataccgg accccaccac
    10321 gcgcatactt gagcatgatc tgttgatcgt ggaggggaag atcaacgatt tgttggaggt
    10381 ccgcgaaagg gagggcattt ccattcgcgc cgacctgctg atcgatcggg agttgcaaag
    10441 cgagcgcttt aaactggccg agattctcgt gacacccgag agcgaattcc ttaactcgac
    10501 attgaaagag gcaaagtttc ggcagcgtta tgggttgacc gtactcgcca tcaaccgcac
    10561 cgggtattcg gtgaacgaga agcttggccg ttttgtctta agaagcggtg atgtcctgct
    10621 ggtacaagga tccgtggaaa cgatctcgta tcataaacgg agcaagcgca tgcaggtgat
    10681 gtcggacttc atgccattgc tgtatcgcaa gagcaagggt ttgctgacac tcggattgtt
    10741 tgttgcggcc gttattatag gatcactcgg ctggttgcct ttgtcggtga cctt
//
LOCUS       DAOTON010000005        10691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_5800_length_10691_cov_3.454964, whole genome shotgun sequence.
ACCESSION   DAOTON010000005 DAOTON010000000
VERSION     DAOTON010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10691
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5800_length_10691_cov_3.454964"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="P5292_00235"
     CDS             <1..559
                     /locus_tag="P5292_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS37580.1"
                     /translation="IALVAVYGTIFFQIFASIPQYFKTVSGYDEDRIGLLMALNGILV
                     VIIEMPLIAKLEKSKKIFRFIVIGVFCLPAAFALLYFGQHRLSFAILYTFVITLSEIF
                     AMPFMMNYALSRPKKERQGQYSALYSIAYGIATIAAPSLGLGIADRYGFDRLFVILIV
                     SSLFLSLGFLWMAGKEKTEHTESGG"
     gene            652..1758
                     /locus_tag="P5292_00240"
     CDS             652..1758
                     /locus_tag="P5292_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37581.1"
                     /translation="MKSFSTLFLLTCLLLVAFASAQTTRRVVIEEFTGAWCGACPDAA
                     IKIEEIEHDYPDNTIGLAWHEWDDLEIPEWTPVKTLFGVSSYPGGAVDRYKFTGASRI
                     ILSHLSFRTRFLNRYNTPAIVSVGMRDLSFDGAAYHFTVDANFSAAPTPGASLRVNVI
                     IKEDSIPAVGSLEQHNYYSTIQNGNNPLVNWYHNEAIRRCLGGPWGVTGGLPPVAVTG
                     ITYSSDFSFIPDPSWNLDQLHLVAIVTYDGDTSLNQKEILNAETVRLRDWMLSTNLNT
                     PSPAALSATLAPNPVTRFGRTEVAYDLSITGEVRYELFNQLGERVIDPVISYEVAGRH
                     TWQWSPRGLPAGIYQLQLSTAGGSFFATRICVVD"
     gene            1821..2411
                     /locus_tag="P5292_00245"
     CDS             1821..2411
                     /locus_tag="P5292_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37582.1"
                     /translation="MPYLLSAQDIAGDYSLHGFREVASGLRLSADHTFEFFYTYGASD
                     RQASGTWSLDSGTVVLQGSKKKGQDFKLRKSAREGGPGMVIKISDKNTYLQQSVVCFA
                     FTDRDSMIEESGIDGIVRFNADGIERIELIHAVFPDEPTIIDCSNDPSNYFEFTLNPS
                     LGEVVFDRVAVSLEGQSLRFSNKYLFGDRVAEFTKP"
     gene            complement(2505..2783)
                     /locus_tag="P5292_00250"
     CDS             complement(2505..2783)
                     /locus_tag="P5292_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRS37583.1"
                     /translation="MSKRRPELLIDDILESCSKILAYTDGLTYEDFCKDNKTIDAVIR
                     NFEIIGEATGRLPEVFKDQHTEIDWHKIRGFRNRIVHDYFGIDYSIVW"
     gene            complement(2776..3069)
                     /locus_tag="P5292_00255"
     CDS             complement(2776..3069)
                     /locus_tag="P5292_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRS37584.1"
                     /translation="MSAQLNLVKTALIAMKAELAAKFHVNSIGLFGSIVRDDFTSTSD
                     IDIIVEFSQPIGIEFVELADLFEQKLNRHVDLVSRNGIKDRYFKAIEAEIVYV"
     gene            complement(3160..3903)
                     /locus_tag="P5292_00260"
     CDS             complement(3160..3903)
                     /locus_tag="P5292_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37585.1"
                     /translation="MKYLLTILTTIFFLENTAQSADRFYFQWGWNKETYSKSTIHFTN
                     GDEYDLYWEKAKAHDKPDFNYILSPQITIPQFSFRLGVRIKDKWFVELNHDHAKYVVE
                     RNQIVRMYGTRNGQIVDENVELRWSEFNFEHTNGANFWMLMAAREFDSLFASPKFGAF
                     AKAGGGLLYPRTDVTLFGKRFNNNFHVAGYLVGVEGSFRYYPFRHFYLDLSGKTGYAN
                     YTNALTVEGGKAQHSFVFLEGIFSLGVRI"
     gene            complement(3988..5376)
                     /locus_tag="P5292_00265"
     CDS             complement(3988..5376)
                     /locus_tag="P5292_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37586.1"
                     /translation="MRHVLLSCLCFLLLQPTAYTQHLWGCYSIGGTYGAGTFFRMDLG
                     GGNLTRYFNCGVGDSGSPQTTFLPQPGNFYYGMTVSGGQYVRGDIYRFDVSSLQYTSL
                     FSMDTASGYYPRGPLYKAGDGNLYGLTSNGGANGLGVLFRFDPSTNVYTKLHEFDGPT
                     GQYPDGGVVQAANGKIYGMTTTGGSAGGGGVIFSWDPATSTYLVEYSFSFSDGFTPFG
                     TLTEYNGFLWGLTFGGGATGNGTLFRFDPSTSTHLLKYSFDGTNGSAPQGNLTLGANG
                     ILYSAASQGGTDSYGVLFSFDTNNDQYTVLHDFDPLEGSRPRGQLCYASDGKVYGTCF
                     NGGANSFGSIYSYDPVTNIYATIDDGDFTDGGTPYAGLIEYAFTIGIEETDPNVVRFF
                     PNPASRKINYAVETGALPDQILVYDPLGRLCSTLLPVTNTGMLELEPGCYTLLIDWTD
                     RRSSQRLVVTGE"
     gene            5722..9126
                     /gene="ileS"
                     /locus_tag="P5292_00270"
     CDS             5722..9126
                     /gene="ileS"
                     /locus_tag="P5292_00270"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRS37587.1"
                     /translation="MSTNYPEYRNLDLPGIAREVLETWEREQTFEQSVSSREGRPAFT
                     FYEGPPSANGLPGIHHVMARAIKDIFCRYRTLKGFQVKRKGGWDTHGLPIELSVEKTL
                     GIRKDDIGKTISIEDYNKACRREVMKYKDVWDDLTRKMGYWVDLEHPYITFENEYIET
                     CWYLLSQFYKKGLLYKGYTIQPYSPAAGTGLSSHELNQPGTYKNVKDTSAVAMFHVPG
                     DAIAKAFGLSAAEGKTAFLAWTTTPWTLPSNTALAVGAKIVYVAVATYNQYTHEKVTV
                     VLAKDLLGKYFPEKNAELSFDDYKSGDKAIPFRVLGETNGKSLDGIRYEQLLPYAQPT
                     DGDAFRVVLGDFVTTEDGTGIVHIAPSFGADDFRVAKQNGVGSLTLVDKQGRFIAEVT
                     DFAGEYVKEQYYTEEEKEAERKKQGGEKYLSVDDRIVIKLKKEGKAFKSERYEHNYPH
                     CWRTDKPVLYYPLDSWFIRTTALKDRMAELNTTINWKPESTGTGRFGNWLENLVDWNL
                     SRSRYWGIPLPIWRSEDAQEELCIGSIEQLREEMKKSIAAGFMDAKHLEHIDPAQPDF
                     DLHRPYVDNIVLVSPTGKKMFREADLIDVWFDSGAMPYAQFHWMGDPATAPFTKDTPA
                     FPADFIAEGVDQTRGWFFTLHAIAVMLFDSVAFRNVVSNGLVLDKNGNKMSKRLGNAV
                     DPFETLKQYGPDATRWYMISNAAPWENLKFNVDGIAEVQRKFFGTLHNTYAFFALYAN
                     IDGFRFNEAEVPVAERTELDRWILSELHTLVRTVDASYADYEPTRACRAIADFVSEHL
                     SNWYVRLSRRRFWKGDYTSDKIAAYQTLYTCLETVAVLMSPVAPFYADRLFRDLNAVT
                     GRHDVASVHLALFPQSDDKIIDKALEERMELAQQLSSMVLSLRKKVNIRVRQPLARIM
                     IPAEDERFRATLESVRPLVLAEVNVKNIEYITDTAGVLVKKIRPNFKVLGKKVGGLMK
                     DTAVAIGAMNQADILRMESEKSFELKVQGQTVTLSPEDVEILSEDIPGWQVASEGRLT
                     VALDTTLNEELREEGIAREFINRVQNLRKDKGYEVTDRIELKVQNHAAIRPSLLNNKD
                     YICAEILAASLDLVDRLEGDNADEVEVDDNIQTRVQIRRHTPGQVN"
     gene            9555..9971
                     /locus_tag="P5292_00275"
     CDS             9555..9971
                     /locus_tag="P5292_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRS37588.1"
                     /translation="MARTATPAKEEDPGKTRYSDKELEEFRQIIVKKLEEARHELNVL
                     QAQLTAANEHGTDDTASTFKMLEDGSESLAKEEAGQLAGRQKKFIEQLENALIRIENK
                     TYGICRVTGKLIPKERLRAVPHTTQSIEAKLNQYRD"
     gene            10195..>10691
                     /locus_tag="P5292_00280"
     CDS             10195..>10691
                     /locus_tag="P5292_00280"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HRS37589.1"
                     /translation="MPSIPTTRLRLAGIIIFLVLLIDQVVKFWVKTTMYLGEEHRVFG
                     HWFYIHFTENYGMAFGLEFGGMAGKFLLSAFRILACIAIGWYIVHLARHRAHPGLMVC
                     FALIFAGAVGNIIDSAFYGMIFNDSTDGIARFLPPDGGYAGFLQGRVVDMLYFPLISG
                     RFPDWL"
BASE COUNT         2558 a         2970 c         2822 g         2341 t
ORIGIN      
        1 catcgcgctg gtggccgtgt atgggaccat cttcttccag atcttcgcca gcattccaca
       61 atacttcaaa accgtcagcg ggtacgacga agaccgcatc ggactgctca tggcattgaa
      121 cgggatactg gtcgtgatca tcgagatgcc gttgatcgcc aagctggaaa agtcgaagaa
      181 gatcttccgg ttcatcgtca tcggcgtctt ttgtttacct gccgcgttcg ctttgcttta
      241 tttcggtcaa caccgactct ccttcgcgat actgtatacg tttgtgatca ccctctcgga
      301 gattttcgcg atgccgttca tgatgaacta tgcgctttcc cggccgaaaa aagaacggca
      361 gggacaatac tccgcgctct attcgatcgc ctacgggatc gcgaccatcg cggcgccatc
      421 attggggctt gggatcgcgg accgatacgg tttcgatcgc ttgttcgtca tcctgatcgt
      481 ttcatccctt tttctgtcgc tgggtttttt gtggatggcg gggaaggaga agacggaaca
      541 taccgaatcc ggcggctgag cagtgctttg aatcgttgga acgcggattg cgcggaggtc
      601 acggttttgc ggatcgactg aattcctata tttggatata gcatttatcg catgaagtcg
      661 ttcagtactt tgtttctgtt gacctgcctg ctcttggtag cgtttgcttc cgcccaaacc
      721 acccgccggg tcgtcattga agaattcacc ggcgcatggt gcggagcgtg cccggatgct
      781 gcgatcaaaa tagaagagat cgagcatgat tatcccgata atacgatcgg cctggcctgg
      841 cacgaatggg acgatctgga gatccccgaa tggacgccgg tcaaaaccct cttcggggtg
      901 tcgagctatc ccggtggagc agtggatcgt tacaagttta ccggagcatc ccggatcatc
      961 ctgtcgcatc tttcgtttcg tacccgtttc ctgaaccgct acaacacccc ggccattgtc
     1021 tccgtgggca tgcgggatct gtcatttgat ggggcagcct accattttac cgtcgatgcg
     1081 aatttttcgg cggctcccac accgggcgcc tccttgcgcg tgaatgtcat catcaaagaa
     1141 gacagcattc cggctgttgg atcgctggaa cagcataatt attattcgac gatccagaac
     1201 ggcaataatc cgctggtgaa ctggtaccac aacgaagcca ttcgccgttg tcttggcggt
     1261 ccgtggggtg tgaccggcgg cttgccgcca gtagcagtga ccggaatcac gtattcgtcc
     1321 gatttcagtt tcatccctga tccttcctgg aacctggacc agctgcacct ggtcgcgatc
     1381 gtgacctacg atggcgatac gtcgctcaat caaaaggaga tcctcaacgc ggaaacggtg
     1441 cggttgcgcg actggatgtt atcgaccaac ctgaacacac cttcgcctgc tgctttgagt
     1501 gccaccctgg ctcccaatcc cgttacgcgt tttggtcgta ccgaagtcgc ttacgattta
     1561 tccatcacgg gagaggtccg ttacgagctg ttcaatcaac tcggtgagcg tgtgatcgat
     1621 ccggttatca gctatgaggt cgccgggcgg catacctggc aatggtcgcc gcgcggtctt
     1681 cctgccggga tctatcaact tcagttatca actgccggcg gatccttctt cgctacgcgc
     1741 atatgcgttg tggactgagt atgaatgctg aagccttgcg cagtcgcgta atccgtttac
     1801 tactcctatt ggctgtaacc atgccttatt tgctatcggc ccaagacatt gccggcgact
     1861 attccctcca cggctttcgt gaggttgcct ccggattacg gctcagcgcg gatcacacgt
     1921 ttgagttctt ctacacttac ggagcctccg accgtcaggc ctccggtacc tggtcgctgg
     1981 actctggaac ggtcgtgctc cagggaagca agaagaaagg gcaggatttc aagctcagga
     2041 aaagcgcacg ggaaggtgga cctggaatgg tgatcaagat ctccgataag aatacgtacc
     2101 tccaacaatc cgtcgtctgc tttgccttca cagatcggga ttcaatgatc gaagaaagcg
     2161 gtatcgacgg cattgtccgg ttcaatgcag atgggataga gcggatcgaa ttgatacacg
     2221 ctgtgttccc ggacgaacct accatcatcg attgctccaa cgacccttcc aactatttcg
     2281 aattcacgct caatcccagc ctcggtgaag tggtgttcga ccgggtagca gtaagtctgg
     2341 aaggtcaatc cctgcggttc agcaacaaat atctttttgg cgaccgggtg gcggaattta
     2401 ccaaaccgtg aatgtccgtg agagtaaaga acatagtata aggagcatta accccttatg
     2461 gatttcaatt tttctgtcaa agcaggaagg taatggcttc gaatttacca aacgatcgaa
     2521 taatcaattc cgaaataatc atgaacgatc ctgtttctaa atccccggat cttgtgccaa
     2581 tctatttcgg tgtgttggtc tttgaatacc tctggtagac ggcccgtagc ttctcctatt
     2641 atttcaaagt ttctaattac cgcatctatc gttttgttgt ccttgcagaa gtcttcgtat
     2701 gtaagaccgt ctgtataagc cagaatcttg gagcagcttt ccagaatatc atcgatcaga
     2761 agctcgggtc gtcgcttaga catacacaat ctccgcttca atggctttaa aataccggtc
     2821 tttaatcccg tttcgtgaga cgaggtcaac atgccggttg agtttctgtt caaaaaggtc
     2881 tgccagttcg acgaactcaa ttccaatggg ttggctgaat tcaacgataa tatcgatatc
     2941 gctggtggat gtaaaatcgt cacgcacgat agaaccaaat aaaccaatcg aattgacatg
     3001 aaattttgct gccaattcag ctttcatggc aatcaatgca gtcttgacga gattcaactg
     3061 tgctgacata aatcaaatat aatgacaaaa tgcgatcagg cgaaaaattg catcaatcgc
     3121 aatttaagga acgaaatcat tgaaccacag ccgccccgtt cagatgcgca cacccaggct
     3181 aaagatccct tccaggaaaa cgaaggagtg ttgcgccttt ccaccttcaa ccgttaaggc
     3241 gttggtataa ttggcataac ctgttttgcc actgagatcc agatagaaat gccggaacgg
     3301 ataatagcgg aagctgccct caacacccac caggtaaccg gctacgtgga aattgttgtt
     3361 aaaccgtttc ccgaacagcg tcacatccgt ccggggataa agcaggccgc cgccggcctt
     3421 tgcgaacgcg ccgaatttcg gagaagcgaa gagtgagtcg aattcacgcg cggccatgag
     3481 catccagaag ttggcaccat tggtatgttc gaagttgaac tcgctccagc gcaattcgac
     3541 gttctcatcg actatctggc cgttccgtgt tccgtacatg cgcacgatct ggttgcgttc
     3601 caccacatac ttggcatggt cgtgattcag ttcaacgaac cacttgtctt ttatccgtac
     3661 acccagtcga aaactgaatt gtggaatcgt gatctgcgga ctgagaatat aattgaagtc
     3721 gggtttatcg tgcgccttcg ctttttccca atagaggtca tactcgtcgc cattggtgaa
     3781 atggatggta ctcttggaat aggtttcttt attccagccc cattggaaat agaagcggtc
     3841 ggccgattga gccgtattct ctaaaaagaa aattgttgtg aggatggtca gcaggtactt
     3901 catcttgatc gttaaaaacg tggcgaaggt acaagcatcc caattcaagg acaatgatgc
     3961 atgtcctatt ttatcatcct gacgtattca ctctccggtc accaccaggc gctgactgct
     4021 tcggcgatcc gtccaatcga tcaggagcgt gtaacaacct ggctcaagtt cgagcatgcc
     4081 ggtgttggtc acaggcaaca gggttgaaca aagacgcccg aggggatcgt acaccagtat
     4141 ctggtcgggc aaggctccag tttcaacagc atagttgatc ttcctgctcg cggggttcgg
     4201 gaaaaaccgg accacgttcg ggtcggtttc ttcgatgccg atggtgaatg catactcgat
     4261 caacccggca tacggcgttc cgccatcggt gaaatcgccg tcgtcgatgg tagcatagat
     4321 atttgtgaca gggtcgtaag aataaatgga gccaaaactg ttcgcgcctc cgttgaaaca
     4381 ggtgccgtac accttaccgt cggatgcata gcaaagctgc ccgcgtggtc gcgatccttc
     4441 gagcggatcg aaatcgtgca gcacggtgta ttggtcgttg ttcgtatcga aggagaacaa
     4501 cacgccgtag ctgtcggtgc cgccttgcga agcggcgcta taaagtatgc cgttcgcgcc
     4561 aagggtgaga tttccctgag gagccgaacc gtttgttccg tcgaacgaat atttcaacaa
     4621 atgcgtcgaa gtggaaggat caaagcggaa gagggtcccg ttcccggttg cgccgccgcc
     4681 gaaggttagt ccccagagga agccgttgta ttcggtcagt gttccaaacg gcgtaaaccc
     4741 gtcggagaac gagaaactat attcgacgag ataggtcgaa gtggcgggat cccaggaaaa
     4801 gatcacaccg ccgccgccgg cacttccacc cgtggtggtc atgccgtaga tcttcccgtt
     4861 agcagcctgc actacgccac cgtccgggta ttgtcctgtc ggtccatcga actcatggag
     4921 tttggtgtaa acattcgtcg acggatcgaa ccggaacaat acgccgagac cgttcgcgcc
     4981 tccattgctc gtcaggccgt acaggttgcc gtcgccggcc ttgtaaagcg gaccgcgcgg
     5041 ataatagccc gatgccgtat ccatactgaa caggctggtg tattgcagac tcgagacatc
     5101 gaaccggtaa atgtcgccgc gcacatattg gccacccgag accgtcatgc cgtaatagaa
     5161 gttaccgggc tggggcagaa aagtcgtctg tggggaaccg ctatcgccta ctccacagtt
     5221 gaaatagcgg gtcaggtttc cgccacccag gtccatccgg aaaaatgtgc cggcaccata
     5281 agtgccaccg atcgaataac aaccccaaag atgctgggta taggcggtgg gctgcaagag
     5341 taaaaagcaa aggcaggaga gaagaacgtg tcgcatgggg aggagtttaa atcttgaaaa
     5401 aggtacaaaa gggatcgggc atttacggac gatttaacct taataatgag atagttagta
     5461 gagtaaaaat atagcatcat taatgggcta aaattttcaa aaactccaat taatatttat
     5521 ttaaagcaat tattgataat ataaaacatc caactcatcg acttgatgat ggtattccga
     5581 aatcgccatt tttattgaga aaacggcatt ttcgagccct atgctccatc gcttgaccga
     5641 accattcgca aaaccctaaa aatcagcctg tttacccctt cttgttacct ccggaattcc
     5701 gtacctttgc ggcctttatt tatgtcgacc aattacccgg aataccgcaa ccttgacctg
     5761 cccggcatcg ccagggaggt gcttgagacc tgggagcgcg agcagacgtt tgagcaaagc
     5821 gtcagcagcc gcgaggggcg tcccgccttc accttttatg aaggcccgcc ttcggcgaat
     5881 ggcctgcccg gtatccatca cgtcatggcc cgtgccatca aggacatttt ttgccggtat
     5941 cgtacgctca aaggtttcca ggtaaagcgc aaaggcggtt gggataccca cggtctgccc
     6001 atcgaactga gcgtcgaaaa aacgctcggc atccgaaagg atgatatcgg gaagacgatc
     6061 tccatcgaag actacaacaa agcttgtcgc cgcgaggtga tgaagtacaa agatgtttgg
     6121 gacgacctca ccaggaagat gggttactgg gtcgacctcg agcatccgta catcaccttc
     6181 gagaacgagt acatcgagac ctgctggtac ctcctgtcgc agttctataa aaaaggtctg
     6241 ctctacaaag gctacaccat ccagccgtat tcgccggctg ccggtaccgg actgagttcg
     6301 cacgagctca accagccggg cacatacaag aacgtgaagg atacgtcggc agtggcgatg
     6361 ttccatgtgc caggtgatgc gattgccaag gcgttcggtt tgtcggctgc tgaggggaaa
     6421 accgccttcc tcgcctggac gaccactccc tggacgcttc cctccaacac cgcactggcc
     6481 gttggtgcca agatcgttta cgttgcagtt gctacgtaca atcagtacac gcacgagaaa
     6541 gtaaccgtcg ttctggccaa ggacctgttg ggaaaatact tcccggagaa gaacgccgag
     6601 ctgtcattcg acgattacaa atcgggcgac aaagcgattc cgttccgagt attgggcgag
     6661 acgaatggca aatcgctcga cggcatccgc tacgagcagc tattgcccta tgcccaaccg
     6721 accgacggcg acgcgttccg cgtagtgctg ggcgatttcg tcaccacgga agacggcacc
     6781 ggtatcgtac acatcgcacc gagcttcggc gccgacgact tccgcgtcgc gaagcagaat
     6841 ggcgtgggct cgctcacgct ggtcgacaag cagggtcgct tcattgccga ggtcaccgac
     6901 ttcgccggtg agtacgtgaa agagcagtac tacaccgaag aagagaaaga agccgaacgc
     6961 aagaagcagg gtggcgagaa atacctcagc gtcgacgacc gtatcgtcat caaactgaag
     7021 aaagaaggca aggccttcaa gtcggaacgc tacgagcaca actacccgca ttgctggcgt
     7081 accgataagc ccgtactgta ttatccgctc gactcctggt tcatccggac caccgccttg
     7141 aaagaccgga tggccgaatt gaataccacc atcaactgga aacccgaatc gaccggcacc
     7201 ggccgcttcg gtaactggct cgagaacctg gtcgactgga acctttcacg ttcccgttac
     7261 tggggcattc cgctgccgat ctggcgcagc gaagacgcgc aggaagaact gtgcatcggc
     7321 tcgatcgaac aactccgcga ggagatgaag aagtccatcg ccgcgggttt catggacgcg
     7381 aagcacctcg aacacattga tccggcgcaa cccgacttcg acctgcaccg tccgtacgtc
     7441 gacaacatcg tactggtctc cccgacggga aagaagatgt tccgcgaagc ggacctcatc
     7501 gacgtctggt tcgattcagg cgcgatgccc tacgcccagt tccattggat gggcgatccc
     7561 gctaccgctc ccttcacaaa ggacacccca gccttcccgg ccgacttcat cgccgaaggc
     7621 gttgaccaga cccgcggctg gttctttacc ttgcatgcca tcgccgtcat gttgttcgac
     7681 tccgtcgctt ttcgcaatgt ggtgagcaac ggtcttgtgc tcgacaagaa cggcaacaag
     7741 atgtcgaaac gtctcggcaa cgcggttgat ccgttcgaga cgctgaagca atacggaccc
     7801 gacgccacgc gctggtacat gatcagcaac gccgcgccct gggagaatct caagttcaac
     7861 gtcgacggca tcgccgaagt gcagcgaaag ttcttcggca cgctgcacaa cacctacgcc
     7921 ttcttcgcgc tctacgcgaa catcgacggc ttccgcttca acgaagcgga ggtgccggtc
     7981 gcggaacgca ccgaactgga ccgctggatc ctctccgaac tgcatacgct ggtccgtacg
     8041 gtggatgcga gctacgccga ttacgaaccc acccgtgcct gccgcgctat cgccgacttc
     8101 gtgagcgagc acctcagcaa ctggtacgtt cggctcagtc gtcgtcgttt ctggaaaggt
     8161 gattacacgt ccgacaagat cgcggcctat cagacgcttt atacttgtct ggaaaccgtt
     8221 gccgtcctga tgtcgccggt ggcgccgttc tacgccgacc ggcttttccg cgacctcaac
     8281 gcggtgacgg gccggcacga tgtcgcttcc gtacacctcg ccctctttcc gcagtcggat
     8341 gataagatca tcgataaagc gctggaagaa cgcatggaac tcgcgcagca gttgtcgtcg
     8401 atggtgttgt ccttgcgcaa gaaggtgaac atccgggtcc gccagccgct ggcacggatc
     8461 atgatcccgg cggaggacga gcgttttcga gcaacgctcg aatcggtacg tcccctggta
     8521 ctcgcagaag tgaacgtgaa gaacatcgaa tacatcaccg atacagcggg cgtactggtg
     8581 aaaaagattc gtcccaactt caaggtgctg ggtaagaaag tcggcggact gatgaaagac
     8641 acggccgtag cgatcggagc aatgaaccag gccgacatcc tgcgcatgga aagtgagaaa
     8701 tcgttcgaac tgaaggtgca gggacagacc gtcacgcttt cccccgaaga cgtggaaatc
     8761 ctgtcggagg acatccccgg ctggcaggtg gccagtgaag gccgactgac ggtggccctg
     8821 gacacgaccc tgaacgagga attgcgcgag gaaggaattg cccgggaatt catcaaccgg
     8881 gtgcagaatc tgcgcaaaga caagggttac gaagtaaccg accggattga gctgaaagtc
     8941 cagaaccacg cggctatccg gccgtcgctg ctgaacaata aagactatat ttgcgccgaa
     9001 attttggccg cctccctcga cctagtggat cgcctcgaag gcgacaacgc cgacgaggtg
     9061 gaagtcgacg ataacatcca gacccgcgta caaatccgcc ggcacacgcc gggccaggtc
     9121 aactgatcct ccctaacttc ttataattta attgataggt tgccatgagc aagaagacaa
     9181 gtccgaagaa ggccaaaccc gctgctaaag cgaaagcaaa gccggctgcg aaaaaacctg
     9241 ctgccaagaa gcccgccgcc aaggcgaagc cgaaagcgaa accggccccc aagaagcccg
     9301 cggcgaagaa accggtcgcg aagaaagtcg ctcctaaggc agccccgaaa aaggtcgcca
     9361 agcccgcagc aaaagcagcc aagaaagttg tcgttgcgaa agccccggtg aaaccggccg
     9421 tcgtagccaa accgaaaccg gctcccgctc ctgcgaaacc ggcagcatcg gctccgcgtc
     9481 ctgcggcacc tgcacctacc tataaggcag ccaatcctgc acccaaaccc attccgccga
     9541 tcagcaaaac tccgctggcg cggaccgcga caccggcgaa ggaggaagat cccggcaaga
     9601 cgcgctactc ggataaggaa ctggaggaat tccgtcagat catcgtgaag aagctcgaag
     9661 aagcgcgcca tgaactgaat gtgctgcagg cacagctcac ggctgcgaat gaacacggca
     9721 ccgacgatac cgccagtacg ttcaagatgc tggaagacgg ttccgagagt ctcgcgaaag
     9781 aagaagccgg acagttggcc ggtcgtcaga agaagttcat cgagcagctc gagaacgcgc
     9841 tcatccgcat cgagaacaag acctacggca tctgccgtgt aaccggaaaa ctcattccca
     9901 aagagcgcct ccgcgccgtt ccgcacacga cccaaagcat cgaagcaaag ctcaatcaat
     9961 accgcgactg agcacgaaga tccagcataa cgccagacag aatcggttag attcggtctg
    10021 gcgttttcat gaaatgtaga tgcgaggagc gtgaccgaga ccgaggaaaa cgctcataca
    10081 tcccacgtac cgcctaattc ctctcaacta accctccctc ggtctcgctc acgtccctcg
    10141 taactaacct tcctcggtct cggacacgct cctcgcaacc aaccctccct cgccttgccc
    10201 tccatcccaa ccactcgtct ccgcctcgcc ggtatcatca tcttcctggt cctgctcatc
    10261 gaccaggtgg tgaagttctg ggtgaagacg acgatgtatc tcggcgaaga gcaccgggtg
    10321 ttcggccatt ggttctacat tcatttcacg gagaactatg gcatggcgtt cggactggag
    10381 ttcggaggca tggcaggtaa gttcctcctg agtgccttcc ggattctcgc gtgcatcgcg
    10441 atcggctggt acatcgtcca cctggcccgg caccgcgcac atcccggcct gatggtttgc
    10501 tttgcgttga tctttgccgg ggcggtcggc aacatcatcg acagcgcctt ctacgggatg
    10561 atcttcaacg acagcaccga cgggatcgcg cgttttctgc cgcccgacgg cggatatgcc
    10621 ggctttctgc aaggtcgggt ggtggacatg ctgtactttc ctttgatctc cggacgcttt
    10681 cctgattggc t
//
LOCUS       DAOTON010000006        10229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_6126_length_10229_cov_4.260370, whole genome shotgun sequence.
ACCESSION   DAOTON010000006 DAOTON010000000
VERSION     DAOTON010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10229
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6126_length_10229_cov_4.260370"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="P5292_00285"
     CDS             <1..498
                     /locus_tag="P5292_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS37590.1"
                     /translation="CAAVRKNDRRFPVLMLTAWGAIDDKLKGFESGADDYLVKPFEFQ
                     ELLARVKALLKRSEPELSEPSTLRYADITLNLDTREVHRAGNKISLTPKEYGLLEYLL
                     RNPERVVTRAEIASHVWSIQYETGTNFVDVYINFLRKKIDRGYIRKLIHTHVGSGYIL
                     RQPNP"
     gene            495..1874
                     /locus_tag="P5292_00290"
     CDS             495..1874
                     /locus_tag="P5292_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS37591.1"
                     /translation="MNIRIRERLTLQFTLIVTSILLVFATGIYLLSSSYREQEFYARL
                     ETKALTTARLLIEVKEVDNDMLKIIDKNSINAMFSEKVLVYNTANKLIYNSVDDESIP
                     VSDSLLNEIRKSGQRRFHLGLNEAIGLRYVEGDDEYVILASALDKYGRSKLNNLFWVL
                     IFGFIASVIVTVIAARIYSARALKPMSDIVRQAERISFTSMGIRIPEGNGKDEIAQLA
                     ITFNKMLDRLQSSFESQRAFVSNASHELRTPLTSITGQLEVTLIRDRSGEEYRNVLRS
                     MLDDTRDLNQLLNSLLYLARANADDRLVRISKVRMDELIWQILGKLRKQHPDYEVELT
                     YSEKIEDENSVVIEADEDLLRVAVSNLIENGCKYSDPHRCIVSLEPQRDRLHLRIEDA
                     GIGIDPADLPHVFEPFYRSMEVARKGGHGLGLPLAKRIVQIHHGDLSLSSTPDTGTRV
                     DVFLPYLQS"
     gene            complement(1915..2286)
                     /locus_tag="P5292_00295"
     CDS             complement(1915..2286)
                     /locus_tag="P5292_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37592.1"
                     /translation="MPDETTAVQLQTATAVEEESQVIVFCHYRATGPDQLIRIWSTTV
                     LSDRSSSHESKLLFTDNVAVAPQWMHVPNGSTARFTLIFSGLPRSCTSFDLIERIPEP
                     GGFEVRNIGRNKTDVYEVEFL"
     gene            2437..2949
                     /locus_tag="P5292_00300"
     CDS             2437..2949
                     /locus_tag="P5292_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRS37593.1"
                     /translation="MARVRYTTIIQKFREKGEKSGWTYIDVPADVANRLKKGQRTSFR
                     VKGKLDDSPIEKVALIPIGNGDFILPLNAALRKAIGKREGAGLTVDLQLDESVYQINA
                     DLRACLEEEPAARKIFDKLPGSHQRYYSKWIDGAKTEVTRARRIATTIDGMIKGWSFA
                     EILRNASGKK"
     gene            complement(3005..3319)
                     /locus_tag="P5292_00305"
     CDS             complement(3005..3319)
                     /locus_tag="P5292_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37594.1"
                     /translation="MKTLILSLALAGITSLTASAQTSTPAIGQKQVNQHERIKDGVDS
                     GQLTKKEARHLRARQAKIRHDKRVAKMDGVVTPAERKQLHKEQRRASRAIHRQKHDEQ
                     HR"
     gene            3576..5792
                     /locus_tag="P5292_00310"
     CDS             3576..5792
                     /locus_tag="P5292_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS37595.1"
                     /translation="MQPTLIPAIAEAFLRQYGFSQEADRLVALQTRLTHEQEFSLDEF
                     LAKLTEFGSQFNLVFLSNDLPSGELSKLALSGSYPFLVFSKKGTSLYPILVHAGKGER
                     FATAFTAQGEQTMRIERVEDLGDLQRHDGLSEADSIVVITCFPHSNFEEPEELRAIDV
                     GSVRFKLFSKLLKLLQPERKEILYIFLYAILIGIISLSLPLGVQSLIGFISSGQVVTS
                     VVVLIAFILLGIVFSGIMTIYQLELVEFLHQKLFTRTAFSFASRIPRIRLESVLQYYP
                     PELMNRFFDTITLQKGLPILLIDITAATLQVIFGILLLSFYHPLFIVMGSVLVFLLIF
                     ILRMTGPKGLATAMTESDYKYRVANWLEEIARTLSTFKLAGHTSFALDKTDHLVSNYV
                     QAREDHFRVLKKQYYSFVVFKTIVTAMLLILGAVLIVDRQINLGQFVAAEIVIIMTIN
                     SIEKIILKIDKVYDVLVGVEKITKVTSLPVEEYKGIDLVVPKNSGLSIRTENLGYRFP
                     DRAQPTLNGVSLKVDASERIAITGYNSSGKSTLINLILGLYDSYSGNIQFNGVSLREL
                     NKASLMNHVGDFVSEEALFDGTILENISIGRKGVTMEDVLWACKAAGLNDFLSGLPEG
                     LNTRLVGGPVRVPESMVKKIVIARNIVEHPALLILDDFLLGVERREKKRILELILDRN
                     YGWTVILISNDPVVLTAVDRVVVMKEGRIVDEGTFDALSSRSEEFRELIQNYPASL"
     gene            5796..7184
                     /locus_tag="P5292_00315"
     CDS             5796..7184
                     /locus_tag="P5292_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRS37596.1"
                     /translation="MGQNIPPVNDRSLPFKAASLVRDTRYAKKFARITSGIFILLFLL
                     LFMPWTQNIRSSGSVTTYLPQDRPQSLQSVIPGRIERWYIREGQFVHKGDTIVRISEI
                     REKFFDPKLLERVSDQIKAKEGSQSSTAAKVSALAQQISALQKEKVLSLEKARNKVKQ
                     AELKILSDSAELQAIRVDMEIARVHARRADSLLKRGLIALTEQERRNLKQQETTAKQV
                     AVENKLLTSRNELINANIELGSLEAEYSNKISKAESELNSALSYLYETQAEIAKMNID
                     FSNLSIRSSFYFVTAPQDGYVVQAKVSGVGENIAEGDVICTIMPSDPSLAIQLYVQPM
                     DIPLLEKGRKVRLQFDGWPALVFSGWPNYSFGTFGGVVAVIDNFDTQGKYRILVVPDP
                     DDTPWPEQIRVGSGTFGWIMLKDVPIWYELWRQFNGFPPDYLGTAKVSTNPVDVKSAG
                     EKKKPGKSEEKE"
     gene            7184..8596
                     /locus_tag="P5292_00320"
     CDS             7184..8596
                     /locus_tag="P5292_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS37597.1"
                     /translation="MKRSTLLVLLTLCTLLATAQDSTRTLSLREVYRLVLDHHPVAKQ
                     AALLPDAARAELRMARGMFDPVLKSGFDEKNFEGKEYWKSWENSLVVPVWFGTDIKAG
                     FDNSRGPYLDSERVTPGQGLSFLGVSVPLGQGLLIDERRATVRQAQLLGTLAAAEQVK
                     VINKLLLQVNKDYLDWTYAFTRLQWHEESLRLAEVRFDAIRMRVLFGDQPAIDSLEAL
                     IEVQNRENIRNQSLLDFTNARLIMSNHLWSPEGAPLEMTAEVVPTFEGTVIDTLDAVS
                     LNAMLDSANSNHPELVKLRTKLQGLEIEKRWAAEKLRPKLNLDYNFLAPRADGLLENG
                     LQPIYQENYKMGLSFRMPLFLREERGKLNLMKIKMNQTEQEQLQTNREITNQVRAAYN
                     ELNNLGLQLRIQQQQLENAEKLLAGELYKFENGESFLFLVNSRENAVINSRIKLAELK
                     AKYGKSRAFLSWSAGRLIAE"
     gene            complement(8606..>10229)
                     /locus_tag="P5292_00325"
     CDS             complement(8606..>10229)
                     /locus_tag="P5292_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37598.1"
                     /translation="NAGASLTINPGITLSVCGDFINNGSLVMLPGSQVRFVGSGNQAV
                     VGNFTGANRFANFTMAKPSGLLVLDSDIDIEENDSLLIGGQFDPASHRIRLKKNFYNA
                     DGANTHVSPAAGTTYEFNGNAVQVFRNDGSDIVLDTVVMNQAPASSLQLSPSGNSELI
                     VAGRLTLTSGKIVTGTEEVDVINGDNAAVTSGNVNSYVEGKLIRALLPNATGIYDFPV
                     GNAARGYELARLDFTSPTQIPEIEAEFFSWGAVPNGPASSECVYADYSLNPSLNHGYW
                     TLTASSNGNTGVYDITLFNRSYTNAVVGGGWSVMKRSPSGSGPWFLDGTCVLTSTITA
                     STRTGLTGFSDFATVQSSFPLPIELLSFTAKLVGDQVLTQWSTATETNNDYFTVEHST
                     DGINFESVGVVDGAGNSVHTLTYAYVHEYPVRGVNYYRLKQTDYDGRSGYSDVVAVKV
                     VRAPSGISVYPSPAIQDITLEFSSTRGEAASIQVTDVIGQLVLEEAITLDKGMVSRIL
                     PVKMLADGLYTITIYSDHDCWRTTFVKGEARP"
BASE COUNT         2413 a         2737 c         2764 g         2315 t
ORIGIN      
        1 tgcgcggcgg tgcggaagaa tgatcgcagg tttccggtcc tcatgctgac ggcatggggt
       61 gcgattgacg acaagctcaa aggatttgaa agtggagccg atgactatct ggtcaaacca
      121 ttcgagtttc aggaattact ggcccgcgtt aaggccttac tcaagcgctc ggagcccgag
      181 ttatcggagc cgtccacact ccgttatgcg gacattaccc tgaacttgga cacgcgcgaa
      241 gtgcatcgtg ccggtaataa gatctccctg actccgaaag aatacggtct gttggaatat
      301 ctcctgcgca atccagaacg cgtcgttacg cgggcggaga tcgccagcca cgtctggtcc
      361 atccaatatg agaccggtac taatttcgtg gatgtttaca tcaattttct gcgtaagaag
      421 atcgatcgcg gatatatccg taaattgatc catacgcatg tcggcagtgg gtacatcctc
      481 cggcagccga acccatgaac attaggatca gggagcgcct gacgctccag ttcaccctga
      541 tcgttacttc cattctactg gtatttgcga ccggcatcta cctgctgtcg tcgtcctatc
      601 gtgagcagga attctatgcc cgtctggaaa ccaaagcgct gacgaccgcc cgcctgttga
      661 tcgaggtgaa ggaggtggac aacgacatgt tgaagatcat cgataagaat tccatcaacg
      721 cgatgttcag tgaaaaggtg ctggtataca acaccgccaa caagctgatc tacaattccg
      781 tcgatgatga atcgattccg gtttccgaca gcctgttaaa tgagatccgg aaatccggac
      841 aacgacgatt ccacctcgga ctgaatgagg cgatcgggct tcgctatgtt gagggggatg
      901 atgaatatgt tatccttgct tccgcacttg acaaatacgg ccgaagcaaa ctgaacaacc
      961 tgttctgggt actcatcttc ggattcattg cgagtgtgat cgtcacggtc atcgccgcac
     1021 gcatctattc ggcgcgtgcg cttaagccga tgtcggatat cgtccgacag gccgaacgca
     1081 tcagtttcac cagcatgggc atccgtatac cggaaggcaa tggaaaggat gagatcgcgc
     1141 aattggcgat taccttcaac aagatgctgg accggctcca gtcttccttt gagtcccagc
     1201 gcgcattcgt atcgaatgcc tcccacgaat tgcgcacccc tcttacatcc attaccggtc
     1261 agcttgaagt caccctgatc cgggaccgca gcggggaaga gtaccggaac gtcttgcgtt
     1321 ccatgctgga cgacacccgt gatctgaatc aactgctgaa cagcctgctc tacctggccc
     1381 gggcaaacgc agacgaccgc ctggtacgca tcagcaaggt ccgcatggat gaattgatct
     1441 ggcagatcct tggtaaacta cggaaacagc atcccgacta tgaggttgaa ctgacctata
     1501 gtgaaaagat cgaggacgag aacagcgtcg tgatcgaagc ggacgaagat ctcctgcgtg
     1561 tggccgtttc caacctcatc gagaacggtt gtaaatattc cgaccctcac cgctgcatcg
     1621 tatccctgga accgcagcgc gatcgtttgc accttcggat agaggatgcc gggatcggca
     1681 ttgacccggc tgatctgccg cacgtgtttg agccattcta tcgctccatg gaagtggcga
     1741 gaaaaggcgg tcacggactg ggtcttccgc tggctaaacg gatcgttcag atccaccacg
     1801 gtgacttgtc attatcttct accccggata ccggaacccg tgtggatgtt tttcttccct
     1861 accttcaatc gtaaacgaac gtttccgaag gggtatcctg ttttagtaaa atcattaaag
     1921 gaattccact tcgtaaacat cggtcttgtt ccggccgata ttccgaacct cgaaccctcc
     1981 cggttccggg atccgttcga tgagatcgaa gctggtacag gaacgcggca gtcctgaaaa
     2041 aatgagggta aagcgtgccg tgctcccatt gggcacatgc atccattgag gcgctacggc
     2101 gacgttatcg gtgaacagca gtttgctttc atgcgatgag gagcgatccg ataagaccgt
     2161 agtactccag atcctgatca attgatccgg tccggtcgcc cggtaatggc agaaaacaat
     2221 tacctggctc tcttcttcaa ctgcagtagc ggtttgcagt tggaccgctg tcgtttcatc
     2281 cggcatgcgg taaagttaag ggaatatcaa ccgcaatcag ttcaacgaat tgatgtcgat
     2341 gagtctcttg atatgacttt cattggatta gttttgtcag gacataatcg aggtcccaaa
     2401 aatattcggg tactgataat aatcttgacc ctcaacatgg cacgcgttcg ttatacgacc
     2461 atcatccaga aattccggga gaagggtgaa aaatccggat ggacgtacat tgatgttccg
     2521 gccgatgtgg ctaaccggct taaaaagggt caacgaactt cgttccgcgt caaaggaaaa
     2581 ctggatgatt cccccattga aaaggtcgcg ttgattccga tcggcaacgg ggatttcata
     2641 ttgccgttga atgctgcgct tcgcaaagcc attggtaaaa gagaaggtgc cggtttaacc
     2701 gtggacctgc aattggatga atccgtttat cagatcaacg ccgacctgcg ggcctgcctt
     2761 gaagaggaac ccgctgccag gaaaattttc gataagcttc ccggctctca tcagcgttat
     2821 tattccaagt ggatcgatgg cgcgaaaacc gaggtgacgc gtgcccgacg catcgccacc
     2881 acgatcgatg gtatgatcaa gggttggagt tttgcagaaa tcctgcgaaa cgccagcgga
     2941 aagaaatgaa aaaccgggcg aaagagtttc ctcttcgacc ggttgtgcta acgatggcaa
     3001 tctcttagcg atgctgttcg tcgtgcttct ggcggtggat cgccctgctg gcacgacgct
     3061 gttccttgtg tagctgcttg cgttcggccg gagtcacgac accatccatc ttggcaaccc
     3121 gtttgtcgtg ccggatcttc gcctggcgcg cgcgaagatg gcgggcttct ttcttggtca
     3181 gttgtccgga gtccactccg tctttgatcc gttcatgttg gttgacctgt ttttgcccga
     3241 tcgccggggt ggacgtctgg gcggaggccg tgagactggt gatgccggca agggccaggc
     3301 tgaggatgag cgttttcatc gaattggcgt ttaaagatgg cctcgtatta acgaaagcca
     3361 tgccaaaggg cagcccagac ccttccccgg gcgatatcac ttttccgatt gactgatctt
     3421 gtgaacaacc attatctatg taattgatat agagttgatt gtatttcttt agattaattc
     3481 taaaaaatcg ttaatggctg aactatggat ggctttccgg tgtttttctt tgtgcggtaa
     3541 aacagcgttt cattcaccca cactgatgaa ccactatgca accaactttg attccggcca
     3601 ttgccgaagc gttcctgcgg caatatggct tttcgcagga ggctgaccga ctggtagcgc
     3661 ttcagaccag gcttacccat gaacaggagt tttcgctgga tgaattcctg gccaagctca
     3721 ccgaattcgg ttctcaattc aaccttgttt ttctttccaa cgacttgcct tctggcgagc
     3781 tgagcaaact cgccttgagc ggcagctatc cgttcctggt attcagtaaa aaaggaactt
     3841 cgttgtatcc cattctggtc catgcgggaa aaggtgaacg attcgcaacg gcattcaccg
     3901 cacagggtga acagaccatg cgcattgagc gcgttgagga cctcggtgat ctccagcggc
     3961 atgacgggct ttcggaagcc gacagcattg tcgtcatcac ctgcttccct cattccaact
     4021 ttgaagagcc ggaagaactc cgggccatcg atgtcggcag cgttcgtttc aaactctttt
     4081 ccaaactact gaaactgctc cagcctgagc gaaaagagat cctctacatc ttcctgtatg
     4141 cgatcctgat cggcatcatc agcttgtcgc ttcccctggg ggttcaaagc ctgatcgggt
     4201 tcatctcgag cggacaggtc gttacctccg tggtggtgct gattgcattc atcctcttgg
     4261 gaatcgtgtt ttccgggatc atgaccatct accagttgga attggtcgaa ttccttcatc
     4321 agaaactctt tacccgcacg gccttttcgt tcgcctcgcg cattccgaga atacgcctcg
     4381 aatcagtcct gcaatactat ccgccggaac tgatgaaccg cttcttcgac acgatcacac
     4441 tgcagaaagg acttcccatc ctgttgatcg acatcacggc agcgaccttg caggtgatct
     4501 tcgggatcct gctcttgtcg ttctatcatc cgttgttcat cgtaatgggt tcggtcctgg
     4561 tcttcctgct gatcttcatc ctgcgaatga ccggtccgaa agggttggcc accgccatga
     4621 cggagtccga ctataaatac cgagttgcca actggctgga agagatcgcg cgcacgctca
     4681 gcacattcaa actggccggc cacaccagct tcgcgctcga caagaccgac cacctcgtca
     4741 gcaattacgt tcaggcgcgg gaagatcact ttcgggtgtt gaagaagcag tattacagct
     4801 tcgtcgtctt caagaccatc gtaaccgcca tgctgctcat cctgggcgcg gtgctgatcg
     4861 tagaccggca gatcaacctc ggtcaattcg ttgcggcgga gatcgtgatc atcatgacca
     4921 tcaattcgat cgaaaagatc atcctgaaga tcgacaaggt gtacgatgtg ctcgtcggcg
     4981 ttgaaaaaat aaccaaggtg accagtctgc cggtcgagga atacaaaggg atcgacctgg
     5041 tagtaccgaa gaactccggg ttgagcatcc gcaccgaaaa cctcggctac cggtttccgg
     5101 atcgcgctca accaaccctg aacggcgtga gcctgaaggt tgatgcatcg gaacgcatcg
     5161 cgatcaccgg ttacaacagc agcggcaaga gcacgctgat caaccttatc ctgggattgt
     5221 acgattccta ctccggtaat atccagttca acggggtttc tctgcgtgag ctcaacaagg
     5281 cttcgctgat gaatcatgtc ggcgatttcg tttctgagga agcattgttt gacgggacca
     5341 tactcgaaaa catcagcatc ggccgaaaag gcgtcaccat ggaggatgtc ttgtgggcat
     5401 gcaaagcggc cggtctgaat gactttctct cgggtttacc ggaaggcttg aatacgcgcc
     5461 tggtcggcgg gcctgttcgg gtgccggaaa gcatggtgaa gaagatcgtc atcgcccgga
     5521 acatcgtgga acatcccgcg ttgctcatcc tcgacgattt tctgcttggt gtcgagcgga
     5581 gggaaaagaa gcgcatcctg gaattgattc tcgaccgcaa ctacggctgg accgtcatcc
     5641 tgatttcgaa cgatcccgtc gtattgacgg cggtcgaccg ggtcgtggtc atgaaggaag
     5701 gccgtatcgt cgatgaggga acgttcgacg cattgtcctc ccgcagtgaa gaatttcgcg
     5761 aactcattca aaactatcca gcaagcctct aagccatggg acagaacata cctccggtaa
     5821 acgaccggtc gctgcccttc aaagcggcga gcctcgtgcg cgatacgcgc tatgcgaaga
     5881 agttcgcacg aatcacctcc gggatcttca tcctgctctt cctgctgttg ttcatgccct
     5941 ggacacagaa tatccggtcc agcggctcgg tgacgaccta cctgccacaa gaccggccgc
     6001 aatcactgca atcggttatc ccgggaagaa ttgaacgatg gtacattcgt gaaggtcaat
     6061 tcgtccacaa gggcgacacg atcgtacgca tcagcgagat ccgggaaaaa ttcttcgacc
     6121 cgaaactgct ggaacgcgtg agcgaccaga tcaaagcgaa ggagggttca caatcgtcta
     6181 ccgccgccaa ggtctccgcg cttgcgcaac agatcagcgc cttgcaaaaa gaaaaagtgc
     6241 tctccctgga aaaggcccgg aacaaagtga agcaggcgga gctgaagatc ctcagcgaca
     6301 gcgccgaatt gcaggccatc cgcgtcgata tggaaatcgc ccgtgtccat gcccgccgcg
     6361 ccgattcgtt gctcaagcgg ggcttgatcg cgctgaccga acaggaacgc cgcaacctca
     6421 aacagcagga gaccaccgcc aagcaggtgg cggtcgagaa caagttgctt acttccagga
     6481 acgaactgat caacgcgaac atcgaactcg gttcgctcga agccgaatac tccaacaaga
     6541 tcagcaaagc ggagtcggaa ctgaacagcg cgctgtccta tctttatgag acccaggcgg
     6601 agatcgcgaa gatgaacatc gacttctcca acctcagcat acgcagttcg ttctacttcg
     6661 tcacggctcc gcaagacggg tatgtcgtac aggcgaaagt gtcgggtgtg ggcgagaaca
     6721 tcgcggaagg cgacgtgatc tgcaccatca tgccgtccga tccgagtctc gccatccaat
     6781 tgtacgtgca gccgatggat atcccgctcc tcgagaaagg ccgaaaggtc cggctgcagt
     6841 tcgatggctg gccggcactt gtcttctccg gttggccgaa ctattcgttc ggaacatttg
     6901 gcggcgtggt agctgtcatt gacaacttcg atacacaggg aaagtaccgc atccttgttg
     6961 ttccggatcc cgatgacacg ccctggccgg aacagatacg ggtcggctca ggtacgttcg
     7021 gctggatcat gctgaaggat gtgccgatct ggtacgaact ctggcgtcaa ttcaacgggt
     7081 tcccgcccga ctacctcggt acggccaagg tgtcgaccaa tccggtcgat gtgaaaagtg
     7141 cgggcgaaaa gaagaaacct ggtaaatcgg aggagaagga ataatgaagc gctccacgct
     7201 gctggtactc ctgacgctgt gtacactgct ggcaaccgct caggatagca cgcgtacgct
     7261 ttcacttcgg gaagtgtacc gactggtgct ggatcatcat ccggtcgcga agcaggcagc
     7321 gctattgcct gacgctgcca gggcggagct gcgcatggcc cgcgggatgt tcgatccggt
     7381 attgaagagc ggattcgatg agaagaattt cgaaggaaag gaatactgga aaagctggga
     7441 aaactcgctg gtcgttcccg tctggttcgg aaccgacatc aaggcgggtt tcgacaatag
     7501 tcgcggcccg tacctcgatt ccgaacgcgt cacgcccgga caagggttaa gtttcctggg
     7561 ggtcagcgtg cccttgggac aagggctcct gatcgacgag cgacgcgcta ccgtgcgtca
     7621 ggcacaattg ctgggaacgt tggctgctgc tgaacaggtg aaggtgatca ataaactgct
     7681 gttgcaggtc aacaaggatt acctcgactg gacctacgcg tttacgcgac tgcaatggca
     7741 cgaagaatca ctccgcctgg cggaagtccg gttcgacgcg atccggatgc gcgtgttgtt
     7801 cggcgaccag cccgcgatcg attcgctgga agccctgatc gaggtgcaga accgggaaaa
     7861 catccggaat cagtcgttgc tcgatttcac caatgcccgc ctgatcatgt cgaatcacct
     7921 ctggagcccc gaaggcgctc cgctggagat gacggcggaa gtggtcccga cctttgaagg
     7981 aaccgtcatc gatacgctgg atgccgtatc actcaacgca atgctcgatt cggccaacag
     8041 caaccacccc gaactggtca agctccgcac caaattgcag ggacttgaaa tcgagaaacg
     8101 atgggcggct gagaagctga ggccaaaact caacctcgat tataatttcc ttgcgccgcg
     8161 tgccgacgga ctgttggaga acggattaca accgatctat caggagaatt acaaaatggg
     8221 gttatccttt cggatgccgt tgttccttcg cgaagagcgc ggtaagctca acctgatgaa
     8281 gatcaaaatg aaccagacgg agcaagagca actgcaaacc aaccgtgaaa tcaccaacca
     8341 ggtacgtgcc gcctacaatg aattgaataa cctgggactt caattacgca ttcagcagca
     8401 acagttggag aatgcggaaa aacttttagc cggcgagctc tacaaatttg agaacggcga
     8461 aagttttctt ttcctggtga actcccgcga aaacgccgtc atcaacagcc gtatcaagct
     8521 ggcggagtta aaggcgaagt acggcaagag cagggcattc ctgtcgtgga gcgcgggtcg
     8581 cttgatcgca gaataatcgg tacgctcagg gtcgtgcttc ccctttcaca aaggtagtac
     8641 gccagcaatc gtgatccgaa tagatggtga tggtgtacaa tccgtcggct aacattttta
     8701 caggaaggat tcgggacacc attcccttat ccagcgtgat ggcctcttcc aacaccaatt
     8761 gaccgatcac atccgtcact tgaatgctgg cggcttcgcc gcgcgtggag gaaaactcga
     8821 gggtgatgtc ctggattgcc ggactcgggt aaacggaaat gccagaaggg gctcgtacca
     8881 ctttcactgc tacgacatcg gaataacctg accggccgtc gtaatcggtc tgcttcaatc
     8941 gataataatt gactccccgt acagggtatt cgtgcacgta agcatacgtc aaagtgtgca
     9001 cactgtttcc ggcgccgtca actacaccga ctgattcgaa attaattcca tcggtgctgt
     9061 gttccaccgt aaagtaatcg ttgttggttt ccgttgcggt cgaccactgt gttaacacct
     9121 gatcgccgac cagtttagcg gtgaatgaga gcagctcgat tggaagggga aagctcgact
     9181 gtacagttgc gaaatccgag aagcccgtca gtccggtacg tgtactcgcg gtaatggtac
     9241 tggtcagcac gcaggtcccg tccaggaacc agggaccgga tcccgaagga ctgcgcttca
     9301 tcaccgacca gcctccgccg acaacggcat tggtgtagct gcggttgaag agcgtgatat
     9361 cgtagacccc ggtgtttccg ttggaagacg cggtcagtgt ccagtaacca tggttaaggg
     9421 atggattgag ggagtaatcg gcgtagacac actcactgga agccggcccg ttgggaacgg
     9481 caccccagga gaagaactcc gcttcaatct cgggaatctg ggttggactt gtgaaatcca
     9541 atcgcgcaag ttcgtagcca cgagccgcgt tgccgaccgg gaagtcatag atgccggtcg
     9601 cattgggcag aagtgcgcgg atcaactttc cttccacgta gctgttgaca ttgcccgaag
     9661 tcaccgcggc gttgtccccg ttgattacgt cgacttcttc cgttccggtc acgatctttc
     9721 cgctcgtcaa cgtcagccgt cctgcaacga tgagctcgct gttgccggaa gggctcaact
     9781 ggaggctgga tgccggtgcc tggttcatga cgacggtatc gagtacgata tcgctgccgt
     9841 cattgcggaa cacctgtacg gcattaccgt tgaattcata ggtggttcct gccgccgggc
     9901 tgacatgcgt attcgcgcca tcggcattat aaaagttctt cttcaggcgg atccggtgag
     9961 aagccggatc gaactggcca ccgatcagca gggaatcatt ctcctcaatg tcaatatccg
    10021 agtccagcac aagcaagccg gaaggtttcg ccatggtgaa attggcaaag cgattggccc
    10081 ctgtgaagtt tccaacgacc gcttgattac cggagccaac aaagcgtacc tgggaacccg
    10141 gtagcatcac caaactgccg ttattaatga agtcgccgca aacggacaag gtgattccgg
    10201 gattgatggt gagcgacgcg ccggcattg
//
LOCUS       DAOTON010000007         9653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_6505_length_9653_cov_3.862680, whole genome shotgun sequence.
ACCESSION   DAOTON010000007 DAOTON010000000
VERSION     DAOTON010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9653
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6505_length_9653_cov_3.862680"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..195)
                     /locus_tag="P5292_00330"
     CDS             complement(58..195)
                     /locus_tag="P5292_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007817840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HRS37599.1"
                     /translation="MKKSDLPHKICVTCSRPFNWRKKWKRTWEQVRYCSERCRKSRST
                     N"
     gene            complement(212..901)
                     /locus_tag="P5292_00335"
     CDS             complement(212..901)
                     /locus_tag="P5292_00335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS37600.1"
                     /translation="MVAGGRTGIGRALVNRLTNAGHDVLILGRTHPDSLPAGSSFSEV
                     DFGNPEAQLPVVEGPISGLAYLPGTIRLKSFRGLKAHDFQEDLQINALGAIRLLQHLE
                     VNLKGGDGGASVVLFSTVAVQTGMPFHSSIALAKGAVEGLARSLAAEWAPSVRINVIA
                     PTLTNTELASSMLSNDQRKQAAIDRHPLKRILDPEEPASLAEWLLTGASQSVSGKVFS
                     MDAGIGSLKTG"
     gene            992..1675
                     /locus_tag="P5292_00340"
     CDS             992..1675
                     /locus_tag="P5292_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRS37601.1"
                     /translation="MLFNPQVLAELQQTCARYNACLVAVSKTKPVAAIEAVYQTGQRD
                     FGENYVQELSEKAGQLPQDIRWHFIGHLQTNKVKQVVPYAHLIHGVDSKRLLSEINKE
                     AGKSGKMIHCLLQVFIANEATKFGLSESELYSILDQLDAGALPHVQVSGLMGMATNTD
                     EEQVIRAEFRHLKSVFDECKARYPKAAGHFTELSMGMTSDYRIALEEGSTMIRIGSAI
                     FGEREYARK"
     gene            1662..2642
                     /locus_tag="P5292_00345"
     CDS             1662..2642
                     /locus_tag="P5292_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRS37602.1"
                     /translation="MHGSDLHPLGVLFALLTTITWAMGIFPFTQAARRLGIGPLNFFR
                     LVLATCFIAVLAFMFPPSFLAIFGSHYFPAWCWLGLSGIIGLTVGDYFGFAMYAVLGA
                     RLGSVLTTFAPGAALVTGMLIVDEHMTWAGILGMVITIAGVIWISLSKSERERASDTR
                     FGSIAKGIIYGVLAAFCQGAGLVLAKKGMLDQAAAGLPLHPVHATFMRLSIGTLSLLI
                     MILLTRKWNQTVFPILINKEGGIRFAVLGTIFGPTLGVSLSLFTVAYLDAAAAQTIFS
                     LVPVVALFLSYFLIREKITGKSLIGVLVAVSGVIILIWHRQLEALLHLAP"
     gene            2646..4586
                     /locus_tag="P5292_00350"
     CDS             2646..4586
                     /locus_tag="P5292_00350"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRS37603.1"
                     /translation="MQAPRYLPTVLLLACFLIPFFHYFLTCTDSLMFDDAAEFALVIK
                     LGSIAHSPGTPAYILGGMIWNSLSSVFSSSLITRLNLFSVFLVSTAIALLYLALRNFL
                     LLSRQESNASVRLELSAAFAALAFGLGPVTWSWANTIEVYGFQVFAMALLLFGLTSWK
                     RSGRFPYLLLASIGWALGLSNHHLTMIVFSPFIPFFFLSDFFYVAAIESKSTKKKKSA
                     AEAGTFSKLLAAIRSRDFIRFTLIAAVFTLTCYAWMFWRAQSDYAFMFGQPKDLGALF
                     FHVRGGAYTKNITDTSSGMMAVRFPYFLNLTAQQLGIFLIPFFWGVYEMFRSRRRLTA
                     LVVLCFYALLFIYQVRNNQWASTDAYMLLPFLILSIPLAEGIFALTAKPWARILVPIA
                     LVLNFSLPGYAAHNRKTYPVSRDLMELLDASAPRNSVVIISDWTTVIQYYYYRIAENF
                     RPDLTVLNYDIKFTHFRILPILYPAFYTKIQPEYDGFIEALRSEHPYQVVNTGCDLNT
                     PYLLAKFKTLVAKMEKVCSEENRPFLIDPKGFVFYTQQGLISPQRHVSGCFVSSMPPD
                     SISAAKFLPMDFTFLESPLLRHDPSCLDKLVDFQAMLDQHITYYSGGGDTLRARQAER
                     SREKILKLQRELKQSMSFAYKK"
     gene            complement(4594..5013)
                     /gene="aroQ"
                     /locus_tag="P5292_00355"
     CDS             complement(4594..5013)
                     /gene="aroQ"
                     /locus_tag="P5292_00355"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRS37604.1"
                     /translation="MKLLILNGPNLNLLGRREPEIYGNRNFEDYLVELKQHFRDIEFG
                     YLQSNLEGELINALQAADGNWDGIIFNPAGYSHTSVALADTIAAVSTPVVEVHISNIH
                     AREPFRQQSLTARNSRGVIGGFGLDSYRLAVESFRIR"
     gene            5104..6021
                     /gene="xerD"
                     /locus_tag="P5292_00360"
     CDS             5104..6021
                     /gene="xerD"
                     /locus_tag="P5292_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRS37605.1"
                     /translation="MSIFIVVSWKSYINGFKSWLKVERSLSGNSVEAYLHDMSLFEHY
                     LESEQLRLPPADIRLEHLQGFIVRINEQGLSARSQARIISGLKSFFKYCLLENLTQDD
                     PSQLLETPKLGRKLPDTLSVDDINRLIAAIDLSKPEGIRNKAMLETLYSCGLRVSELV
                     NLKLSNLHLEIGFLKVIGKGDKERLVPIGSVATKHLKIYTAEVRNHQTIQHGFEDFVF
                     LNNRGRGLTRVMVFTIIKNLAIKIDLRKRISPHTFRHSFATHLIEGGADLRAVQEMLG
                     HESITTTEIYTHLDRDYLRQSILSHHPRA"
     gene            6018..6869
                     /locus_tag="P5292_00365"
     CDS             6018..6869
                     /locus_tag="P5292_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37606.1"
                     /translation="MSRQRLTFLHAPLPQPQRLFRRLWLIVLFLTMLSPSAEAGRPRY
                     TAPDTIEFSGYRWAVKESMNKRVGPGSNLFSSSRKNVWVDAEGKLHLRVTQQDEKWYC
                     PEIFMVNSLGYGRYTFLLSGLPQPEDKDLVIGMFLYDHLDSMNNHKEIDIEISRWGKS
                     EGQNCQFLLQPYVEEAFRFDIDASRKNRHEIDVRKHTIRFRSLYQGDHGHKSVSLLAR
                     WKCKPSRVYETSNEKVSLNVWIYKSIEPANLKEFEVIVEKFEFKPFKAEKLKPNFKIP
                     WFKKKQK"
     gene            6866..7243
                     /locus_tag="P5292_00370"
     CDS             6866..7243
                     /locus_tag="P5292_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRS37607.1"
                     /translation="MSRGLLSILLLIISNFFMTFAWYGHLQFKKWNVMQQSGLLSIIL
                     VSWFIALFEYLFMVPGNRIGHESNGGPFNLFQLKLLQEAITLVVFSFCAIFIFKTDTF
                     RWNYLAGFLCILLAVFFVFKKWD"
     gene            complement(7250..9484)
                     /locus_tag="P5292_00375"
     CDS             complement(7250..9484)
                     /locus_tag="P5292_00375"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRS37608.1"
                     /translation="MHAQLLTFLLVISTCFSLTGQEPERKVYHIQRCTGKIVLDGEPD
                     EAVWSVCEIAGDFWQSVPVDTSPAVSRTEFRMTYDDDFIYVSAVCYDDLPEKPYIIQS
                     LKRDYSYPVSDGIGVYFDPFNDKTNGFAFTCNPFGVQREGLLANSGFFGVSTDWDNRW
                     FVETRREAGAWKAEIAIPFKSLRYNDGVKQWRVNFSRNNLKRNEGSAWSPVPRVYNIA
                     SLLHTGLLVWDQPPKKPGLNAALIPYGILSHTIDFTRDDETTKPNGGVDAKVAVTSSL
                     NLDLTFNPDFSQVEVDRQQTNLSRFSLFFPERRNFFIENSDLFATFGFSKIRPFFSRN
                     IGLYNGNTIPIAGGARLSGKLDQNWRIGLMDMQTKASAEFDQPAYNFAVGALQRTVLK
                     KSALGAILVNRQTFDKGSEHSGDYNRLAGVDFVYNSKNNLATGKVFYHRTFDTLSGKD
                     KAATAVFFLYRTKNVNLEYNHEYVGENFNPVVGFLGYDKGYFRFENRAAYTWFPEKSA
                     LLSHGPEIYQSQYWNGGFPGGKASEFYIGRPLDYQYRMSYYLRWPNTAVLSVNLNAWY
                     TFLRRAFDPTGTNAERLPAATSYEYRDVNVAFASDFRKPFNYKVGATGGRYFNGRKRT
                     YTLEGVWRIQPIAILSMAYERNEIELPSPYASAYLDLLGPKFEFAFTKSVFFTTFIQY
                     NRQIDNLNINARLQWRFKPMSDFYIVYTDNYFAPEPGAGDTAGKFAIRNRGVVFKLNY
                     WLAI"
BASE COUNT         2222 a         2656 c         2383 g         2392 t
ORIGIN      
        1 caggatgcga tcaaggccat gatcggtacg gaaatgaaac tgtgccactt tttcatgcta
       61 gttcgtactt cgggacttgc gacaacgctc actgcaataa cggacctgct cccaggttct
      121 cttccacttc tttcgccaat tgaacggtcg ggaacaggtt acacagatct tatgcggcaa
      181 gtcggatttc ttcaccggaa tgcgccgaaa gttaacccgt tttcagtgaa ccgataccgg
      241 catccatgct gaaaaccttt ccactgacac tctgagaagc acccgtcaga agccactcgg
      301 caagagaggc cggctcttcc gggtcaagga tgcgcttcaa cggatgccgg tcgatcgctg
      361 cttgttttct ctggtcgttg gagagcatgc tgctggcgag ttctgtattg gtcagggtgg
      421 gtgcaatcac atttatgcga acagagggcg cccattcggc tgcaagagag cgtgccagac
      481 cttccacagc acctttagcc aaggcaatac tgctgtgaaa gggcattccg gtctgcaccg
      541 ccacggtact gaataaaacc accgaagccc caccatctcc acctttcaaa tttacttcca
      601 ggtgctgcaa caagcggatc gcgcccagcg cattgatttg caaatcctcc tggaagtcat
      661 gcgctttgag acctcggaag gatttgagac ggatcgtacc gggcaagtat gcaagtccgg
      721 agatgggtcc ttccacaacg ggtaattgcg cttcaggatt tccaaaatca acttcggaga
      781 aggagctgcc tgcgggtaag ctgtcgggat gcgtccgccc caggataagc acatcgtgac
      841 cagcgttagt caggcgattc acgagcgccc ttcctattcc ggtacgtcca ccggcaacca
      901 ctaattttcc cacgctccat caacattcgg gcggggtaaa tgttttacaa accgaccgcg
      961 aaagccccat tgagccgctt aactttactc catgcttttc aacccccagg ttttggcgga
     1021 actgcagcaa acctgcgcac gctataacgc ttgcctggtc gctgtttcca agaccaaacc
     1081 tgtagctgcg attgaggctg tctatcagac aggtcaacga gatttcggcg aaaattatgt
     1141 ccaggagttg agtgaaaaag ccggtcaatt gccgcaggat atccggtggc acttcatcgg
     1201 tcaccttcaa accaacaagg tcaaacaggt cgttccctat gcgcacctca tacacggcgt
     1261 cgacagtaaa cgcttgttat cggagatcaa taaggaggca ggcaaatcgg gtaagatgat
     1321 ccattgccta ttgcaggttt tcattgccaa cgaggcaacc aaattcggac tttccgaatc
     1381 cgaactgtat tcgatcctcg atcagctgga tgccggagca ctcccgcatg ttcaggttag
     1441 cggcctgatg gggatggcca ccaataccga tgaggaacaa gtcattcgtg ccgaatttcg
     1501 acacctgaaa tctgtcttcg acgaatgcaa ggcccgctac ccgaaagcag ccggacattt
     1561 tacggagctg tcgatgggaa tgacatccga ctaccggata gcgctggagg aaggaagcac
     1621 catgatccgg atcggttcgg caatctttgg cgaaagggaa tatgcacgga agtgatctgc
     1681 atcccttagg tgtactcttc gccctgctga ccacgatcac ctgggcgatg ggaatctttc
     1741 cctttacgca ggctgcacgt agactgggaa tcgggccgtt gaatttcttc cggcttgtcc
     1801 tcgcaacttg cttcattgcc gtccttgctt tcatgtttcc cccttcgttt ctcgcgatct
     1861 tcggttcaca ttactttcca gcctggtgct ggctcgggct atcagggatc attggtctta
     1921 ccgtgggcga ctatttcggc tttgccatgt atgctgtgct gggggcccga ctgggtagtg
     1981 tcctgaccac atttgctccg ggcgcagcac tggtcaccgg catgttgatc gtggacgagc
     2041 acatgacctg ggcaggaata ctcggcatgg tcatcaccat tgccggcgtt atctggatct
     2101 ccctctcgaa atccgagcgc gaacgggcaa gcgacacgcg tttcggttcg atcgcaaagg
     2161 gaatcatcta cggtgtgttg gcagcctttt gccagggcgc cggtctggtt ttggccaaga
     2221 aggggatgct ggaccaggct gcggccggtt tgccgcttca tcccgtccac gcgaccttta
     2281 tgcggctttc catcggaacg ctttctttgt tgatcatgat cctgctaaca cggaagtgga
     2341 accaaaccgt attccccatc ctaatcaaca aggaaggcgg tattcgcttt gccgtcctgg
     2401 gcacgatctt cggtccgaca ctcggagtca gtctgtcact atttacggta gcatacctgg
     2461 atgccgctgc agcccagacc atcttctcgc ttgtgccggt cgttgccctt ttcttatctt
     2521 acttcctgat ccgggagaag atcaccggaa agtccttgat aggtgtactg gtggctgtgt
     2581 ccggagtcat catcctcatc tggcaccgac agctcgaagc actcctccat ttagcgccct
     2641 gacccatgca agcaccacgc tacctcccta ccgttctgct tctcgcttgt ttcctgatcc
     2701 ctttcttcca ttactttctg acctgtaccg attcgctgat gtttgacgat gccgcggaat
     2761 tcgcgttggt catcaaactc ggatccatcg cccattcacc gggcacaccg gcctacatcc
     2821 tgggcggaat gatctggaac agcctgagct cggtcttcag ttcaagcctg attacgcggc
     2881 tcaacctgtt ttcagttttt ttggttagca ccgcaatcgc gttgctctat ctggcgcttc
     2941 ggaatttcct gctcctttcc cgccaggagt caaacgcgag tgttcgactg gaactatccg
     3001 ccgcctttgc cgcactggct ttcggcctcg ggccggttac ctggtcctgg gccaacacca
     3061 tcgaagtata cggattccag gtattcgcca tggcactcct gttgttcggt ttgaccagtt
     3121 ggaagcgatc cggcagattc ccctatctcc tcctcgcttc catcggctgg gctttgggtc
     3181 tgagtaacca tcaccttacc atgatcgtgt tctctccctt cattccgttt ttcttcctgt
     3241 ccgacttctt ttacgttgct gccattgaat cgaaatcaac gaaaaagaag aagtctgctg
     3301 ccgaagctgg tacattttcg aaactccttg cagccatccg ctcacgtgac tttattcgat
     3361 tcacgcttat cgccgcagtt tttacactga cctgttatgc ctggatgttt tggagagcgc
     3421 aatcggacta tgccttcatg ttcggacaac cgaaagacct tggtgcactc ttctttcatg
     3481 ttcgtggcgg agcctatacg aagaacatta ccgacacttc gtcgggcatg atggcggttc
     3541 gtttccctta cttcctcaac ctgacggccc aacaactggg cattttcctg ataccgtttt
     3601 tttggggagt ttacgaaatg ttcagaagcc gcagacgatt gacggcgttg gtcgtccttt
     3661 gcttttacgc tttgctcttc atctatcagg ttcgtaataa ccaatgggcc agtaccgatg
     3721 cctatatgtt actaccgttt ctgattctta gcattccgct tgccgaaggt atctttgccc
     3781 tcactgctaa accctgggcg cgcatcctcg ttcccatcgc cttagtcttg aatttttcac
     3841 tgcccggtta cgccgcacac aaccgtaaaa cgtatccggt aagcagggat ctcatggaat
     3901 tgctggatgc atcggctccg aggaatagtg tcgtgatcat ctcggactgg acaacggtga
     3961 tccagtatta ctactaccgg atcgcggaaa atttccgccc ggacttgacg gttctcaatt
     4021 acgatatcaa gttcacccac ttcaggatac ttccgatcct gtatcctgca ttctacacga
     4081 agatccaacc ggaatacgac ggtttcatcg aagccttgcg ctccgagcac ccctaccagg
     4141 tggtgaacac cggctgtgac ctgaatacgc cctacctcct cgcgaaattc aagacgctgg
     4201 tcgccaaaat ggaaaaggtc tgttccgagg agaatcggcc gttcctgatc gatccgaagg
     4261 gatttgtctt ttatacccaa cagggactga tcagtccaca acggcatgtt tcgggttgct
     4321 ttgtttcgag tatgccaccc gactcgatca gcgcggcgaa attcttaccg atggatttca
     4381 ctttcctgga gtccccgctg ttgcgccatg atccctcctg tctggataag ctggttgatt
     4441 ttcaggcgat gctggaccag cacatcacgt attacagtgg cggaggcgat acgcttcggg
     4501 cccgtcaggc agaacggtcg cgcgaaaaga ttctcaagct ccaacgtgag ctgaagcaaa
     4561 gtatgtcctt tgcttataag aaatgagccc caactatcgg atcctaaacg attccacggc
     4621 aagtcggtaa ctgtccagtc cgaatccgcc gatgactccc cggctgttcc tggcagtcag
     4681 ggactgctgt cgaaaaggct cccgggcgtg aatgttactg atatggactt cgaccaccgg
     4741 cgtgctgaca gccgcgatcg tatctgccag ggcaacagag gtatgactgt agccggccgg
     4801 gttgaagatg atgccgtccc aattcccatc agctgcctga agtgcattga tcaactctcc
     4861 ttccaggttg gattgaagat acccgaattc aatgtcacgg aagtgctgct ttaattccac
     4921 caggtaatcc tcaaaattcc ggttgccgta gatttcaggc tcccgccttc caagaaggtt
     4981 gaggttcggt ccgttcagga tcaggagttt cataggattt taatgcgagc acaaatttta
     5041 cataattatt caaataattc tgtgaataat taggtgttaa atggagcatt ccccatcgtc
     5101 ggaatgagca tttttatcgt cgtgagctgg aaatcctaca tcaatgggtt caaatcctgg
     5161 ctgaaagtcg agcggtcgct ttccggaaat tcggtggaag cgtatttgca cgacatgagt
     5221 ctgttcgaac actacctgga atccgagcaa cttcgcctcc ctccagccga cattcgcctg
     5281 gagcatctgc agggcttcat cgtccgcatc aacgagcagg gactcagtgc cagaagccag
     5341 gcgcgtatca tttccggcct gaagtcattc ttcaagtact gcctgttgga aaatctcaca
     5401 caggatgatc cctcccaact cctggaaacc ccgaaactcg ggcgaaagct gccggatacg
     5461 ctctcagtcg atgacattaa ccgcctgatt gcagccatcg acctgagcaa gcccgagggc
     5521 atccgaaaca aggcgatgct ggaaacactt tactcctgcg gacttcgcgt tagcgagctg
     5581 gtcaacctca agttgtcgaa cctccacctc gagatcgggt tcctgaaggt catcggaaag
     5641 ggcgacaaag aacgcctggt accgatcggg agtgtcgcca ccaagcacct gaagatttat
     5701 acggcagagg ttagaaacca tcagacgatc caacacgggt tcgaagactt cgtctttctc
     5761 aataaccgcg gccggggatt gactcgggtc atggtcttca cgatcatcaa gaatctggca
     5821 atcaagatcg atctgcggaa gcggatctct ccgcacacgt tccggcattc ctttgcaacc
     5881 catctgatcg aaggcggagc cgacctgcgg gccgtgcagg aaatgttggg acacgaatcc
     5941 atcaccacaa ccgaaatcta tacgcacctc gaccgcgact acctgcggca atccatttta
     6001 agccaccacc ctcgcgcatg agccgacaac gactcacctt tctacatgct ccgttaccgc
     6061 agccgcagcg gttgttcagg cggctgtggc ttatcgtatt gttcctgacc atgctcagtc
     6121 cctctgccga agccggtcgc ccccgctaca ctgctccgga taccattgag ttttccggct
     6181 atcgatgggc ggtaaaagaa tccatgaaca agcgggttgg accgggaagt aacctattct
     6241 cttcttcgag aaaaaatgtc tgggtggatg cggaaggcaa actacacctt cgcgtcaccc
     6301 aacaagatga aaagtggtat tgtcccgaga tcttcatggt gaacagcctt ggctatggcc
     6361 gctacacctt cttgctatcc ggactcccgc aaccggaaga caaagacctg gtgatcggaa
     6421 tgtttctgta tgaccatctt gattccatga acaaccacaa ggaaattgac atcgagatct
     6481 cccgttgggg aaagtccgaa ggacaaaatt gtcagtttct gctacaaccc tatgtggaag
     6541 aagctttccg attcgacatt gatgcgtccc gaaagaaccg ccatgagatt gatgtccgga
     6601 aacataccat tcgctttcgt tcactctacc agggcgatca cggccacaag tccgtttccc
     6661 tgctggcgcg ttggaagtgt aaaccttccc gtgtttacga aacaagtaac gagaaagtga
     6721 gcctgaacgt ctggatctac aaatccatcg agccggctaa cttgaaggaa ttcgaagtca
     6781 tcgtcgagaa atttgaattc aaaccattca aggccgagaa gttgaagccc aacttcaaga
     6841 tcccctggtt taaaaagaaa cagaaatgag tcgaggtttg ctgtcgatcc tgctgctgat
     6901 catcagcaac tttttcatga cgtttgcatg gtacggccat ctccaattta agaagtggaa
     6961 cgtaatgcag cagtccggcc tgctcagcat tatcctggtc agctggttta tcgcgttgtt
     7021 cgaatacctg ttcatggtac ccggaaaccg gatcggtcat gaatcgaacg gcggtccgtt
     7081 caatctgttt caactgaagc tgttgcagga agccatcacc ctggttgtat tttcgttctg
     7141 tgccattttc atattcaaga ccgacacctt tcggtggaat tatctggcgg gcttcctgtg
     7201 tattctcctc gccgtctttt tcgtcttcaa gaaatgggac tgagcgtcgt cagatagcca
     7261 accagtaatt caacttgaat accacacccc ggttcctgat cgcgaattta ccggctgtat
     7321 caccggctcc gggctccggc gcaaagtagt tatcggtata gacgatgtaa aagtcgctca
     7381 tcggcttgaa ccgccattgc aaacgcgcgt tgatgttcag gttatcgatc tgccggttat
     7441 actggatgaa ggtggtgaag aaaacactct tggtaaacgc gaattcgaat ttaggtccga
     7501 gcagatccag ataggcgctc gcataaggcg aaggcaattc gatctcgtta cgctcatacg
     7561 ccatcgagag aatggcgatc ggttgaatcc gccatacgcc ctccaaggtg taggtccgtt
     7621 tgcgtccgtt gaaatacctt cctccggtgg cacccacttt ataattgaaa ggctttctaa
     7681 aatccgaagc gaacgccacg ttcacgtccc gatactcata agaagtagcc gccggcaatc
     7741 gttctgcatt cgttccggtc gggtcaaatg cgcgccgaag gaatgtatac caagcattga
     7801 ggttcacgct cagcacagcc gtattcggcc agcgcaggta ataggacatc cggtactggt
     7861 aatccagcgg tctgccgatg tagaattcac tcgcctttcc accagggaag ccgccgttcc
     7921 aatattgact ctgataaatc tccggcccgt ggcttaataa agcgctcttt tcggggaacc
     7981 aggtataagc agccctgttt tcgaaacgga agtatccttt atcatacccc aggaatccta
     8041 ccaccggatt gaagttctct cctacatact cgtggttgta ttccaggttc acattctttg
     8101 tccggtaaag aaagaaaacg gctgttgcgg ctttgtcctt acccgataac gtatcgaacg
     8161 ttcggtgata gaatactttt ccggttgcca ggttgttctt cgagttataa acgaagtcca
     8221 ctccggccag tcggttgtaa tctcccgaat gctcgcttcc cttgtcaaaa gtctgacgat
     8281 tgaccagaat ggctcccagt gctgacttct taagaacggt acgctggagc gcacccacgg
     8341 caaagttgta tgcaggttga tcgaactcgg cagatgcttt cgtttgcata tccatcaacc
     8401 cgatccgcca gttctgatcc agttttccac tcaggcgggc accacctgca atggggatcg
     8461 tattgccatt gtacaagccg atgttacgcg aaaagaacgg ccggatcttg ctgaatccga
     8521 acgtggcgaa caaatcgctg ttctcgatga aaaagttccg gcgctctggg aaaaacaagc
     8581 tgaaccggct gaggttcgtt tgttgccggt ccacttcgac ctgggaaaaa tccgggttaa
     8641 acgtcaggtc aagattcagg gaagatgtca ccgctacttt ggcatccacg cccccattcg
     8701 gcttggtcgt ctcatcgtcg cgtgtaaagt cgatcgtatg gctgagtatc ccgtacggga
     8761 tcagggccgc attcaagccg ggcttcttag gcggctggtc ccagaccagc aagccggtat
     8821 gcagcaggga ggcgatgttg taaacacggg gtaccggcga ccaggccgag ccttcgttcc
     8881 gcttgagatt gttccgagaa aaattcaccc gccactgctt cactccgtcg ttgtagcgaa
     8941 gactcttaaa gggaatagcg atctccgctt tccaggctcc ggcctcccgt cttgtttcta
     9001 cgaaccaacg gttgtcccaa tcggtactca caccgaagaa tccactgttc gccaacaaac
     9061 cttcccgttg tacgccgaac ggattgcagg taaacgcgaa accgttggtc ttatcgttga
     9121 atggatcaaa gtaaacgcca ataccgtcac tgaccggata cgaatagtcc cgcttcaacg
     9181 actggatgat atagggcttt tcaggaagat cgtcatagca aacggcggag acgtaaataa
     9241 agtcatcgtc gtaggtcatc cggaactccg tccgggaaac agccggtgaa gtatccacgg
     9301 gcacactctg ccagaaatcc cctgcgatct cgcatactga ccagaccgcc tcgtcgggct
     9361 ccccgtcgag aacaattttc cccgtacagc gttggatgtg atacaccttt cgctccggct
     9421 cttgtcctgt gagcgaaaag caggtggaga tcaccagtag aaatgtcaac aactgtgcgt
     9481 gcatcgtgga taaacagccc gacaaaggaa caaagtttgg gggctttttc ggctatcttt
     9541 ggccagcttt cttgagcgta tgacgaacct ggaattcaac aagaacgaag atcaataccg
     9601 ccaactgatg gatcagttgc ggaacaagct ttcgaaaatc tcggaaggcg gcg
//
LOCUS       DAOTON010000008         9288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_6799_length_9288_cov_2.846637, whole genome shotgun sequence.
ACCESSION   DAOTON010000008 DAOTON010000000
VERSION     DAOTON010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6799_length_9288_cov_2.846637"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="P5292_00380"
     CDS             complement(<1..642)
                     /locus_tag="P5292_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37609.1"
                     /translation="MNLRLTLFLIAGITLRLAAQSDQDLRFRMSGFHTEPSSARLFDP
                     QSGFNLSAPSGNDAYTCALYFQGRFWTGGFDGPMIRRFSVGGSLVDSLTITGLPDTGT
                     TAITTDGSSLYIAQDQGIYLYEVNPSTLAVTDSILLPLFPKGVAYDSTRDAFWVAYDD
                     YPMVLFDRTGATLQTIPSAVHGLAGIYGLAFDAVTAGGPYLWAFARVTPAGLHR"
     gene            869..1723
                     /locus_tag="P5292_00385"
     CDS             869..1723
                     /locus_tag="P5292_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37610.1"
                     /translation="MHRPLHSLSFVLLFVALQLSVTAQQVCDSVFTPCFNTTSGTGFF
                     LDLEASTDVTIEGFSTMSQGAGTRDVELYYRPGGFVGYETDAAAWTLAGSASGFDPVS
                     AISCPIPVTPLPITFNVCIPAGERYGFYLASTSGSGSFELYDTLTTGTLFSDDGTLRL
                     YVGKGAFAFGAFTGFVTQNKLMQGTIQYSCGCSTGMQHPSAASNVRLFPSIVTREFQL
                     DLSKAQGVYQSARILDSAGRLLDQLELDATATSIIRNVASLSPGVYTLQLTGLKSSAQ
                     LKFVRSSD"
     gene            1891..3495
                     /gene="ricT"
                     /locus_tag="P5292_00390"
     CDS             1891..3495
                     /gene="ricT"
                     /locus_tag="P5292_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRS37611.1"
                     /translation="MACASCGTGTDGKPGGCRSNGTCSTGGCNKLNVYNWLSDMVLPN
                     GQQSFDVVEVRFKGSRKEFFRNKELQDLRVGDMVAVEGSPGHDVGTVSMVGELVRFQL
                     RKKSLAENSESIKALYRKAKPGDVEKWCEMKDKEPATRKRTREIIESLKLNMKLSDVE
                     FQGDGKKATFYYTAEERVDFRELIKRLADEFRIRVEMRQIGMRQEASRLGGIGVCGRE
                     LCCSTWLTDFKTVSTSAARYQNLALNPAKLAGQCGKLKCCLNYELDSYMDAVRDIPAS
                     NIILETEKGRAFHRKTDIFKRLMWYAFSENERRGEGEQAFTGEGNWVVMPVDRVKEIV
                     ALNKEGKKPGELMEGDWLEVEVEEEPDYANVVGQDRIDRMDKKKKKKKKKKSSGSGNA
                     QQAAPTGQAQGGQPQGGQPKERGQQGGQQRQRPDNRPQGGKGGGPRPQQNQQQGGKPQ
                     QQGQQQNQQQRQQQGQRPQQQGQNNNRQQQQGPRPQQQQTPRPQGEQRPQQEQRPQGG
                     NREPRQRPSAPPRQSPGQSTPPPESN"
     gene            3499..3990
                     /locus_tag="P5292_00395"
     CDS             3499..3990
                     /locus_tag="P5292_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HRS37612.1"
                     /translation="MNKPSLRTLLLLTILSLSLSSCDRSVLFEENVRIPDNRWDAANV
                     IKLEADIPDTAQLYNLYVNVRNAGGYPYSNIFLFLNTTLPDGKRSRDTLEVTLADDKG
                     QWLGDGMGDIWDNRALFRERMRFPQSGLYTFEIEQAMRQNPLPQIMDVGLRIERYDDP
                     AGR"
     gene            complement(4003..4512)
                     /locus_tag="P5292_00400"
     CDS             complement(4003..4512)
                     /locus_tag="P5292_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37613.1"
                     /translation="MLRHFLVLACFCYSLNSFANGNSAERAELIHLLEGRAAQFREYT
                     GSLQQKSGIFGQRTKTDLKGSHDRLAAIVETDNEIIRLLDRMLDQRTFERVSMTYDAS
                     RDADRIRNLQAVSDTMTARAARYEAELTKYKSGRSGRSAWLWFFALLALAEGAIILRL
                     RKSSGRRLT"
     gene            4616..6052
                     /locus_tag="P5292_00405"
     CDS             4616..6052
                     /locus_tag="P5292_00405"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRS37614.1"
                     /translation="MRRIILLLCCSIALLAQAQAPKKKPVPAQPASPKPGPAQTKAIN
                     APAPAPDIKNKVLPYTIHQKKMANGLNVVTIPFESPGVAAFYIVVRVGSRDEIEPGKT
                     GFAHFFEHMMFRGTKKYPKEAYSEALKMTGASANANTSLDRTVYHMTGDASKLEKMFE
                     LEADRFMNLSYSEQDFRTEAGAVKGEYTKNSSSPYTRLNEKLQETAFDQHTYKHTTMG
                     FLKDIVDMPNQFAYSRQFFQRYYRPEYTTILVVGDVTPAGVEALSQRYFGTWQRGTHV
                     NKIPSEPAQKGTRYAHIQKSGFPPYLSLNFRGPGYSDSQKDVAALNILGSALFGENSP
                     LYEKLVINEQKCREIDGGIFPTRDPFLFSADASLVNAADMKYVQEQINLALAGAKEAP
                     MDPARIDQARQNIRNSFAMRIDNPTSIAEAVSNFIWLTGDPESINRYYDQYEQVTPYD
                     VQRVAQQYCVSEQCTIGTISPSEKSDLQ"
     gene            6049..7617
                     /locus_tag="P5292_00410"
     CDS             6049..7617
                     /locus_tag="P5292_00410"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRS37615.1"
                     /translation="MNRHFTQPMNIRILALLLTLSCCVSTARAFETIELRQPGTNKIA
                     IRLMFRNGSACDPEGKEGLMCLTASLLTEGGTESMSAADITKTLYPMAVRMGSSIDKE
                     VSIVSFEFLKEHFERFYPILRDMVLAPGFRQEDIDRVSSNQLNYLTDVIRQSSDEEFG
                     KKWLEARLFSGTRYAHLVEGSVTGLKGITRGDITDCYKTYFTRGNLLLGIAGDYTTEQ
                     LSLLQSDLQKLPDTPVKCNFGSVTAPSGFQVSIVEKEGAMGSAISAGFPMSLTRSSDQ
                     FAALMIANSWLGEHRKSYSRLYQKIREARSMNYGDYTYIEWYENGGSNMLPPPGTPRS
                     MNYFSIWLRPVQTVTSLKNQYPELKDIEIGHSLFALRMAIREIDQLIKTGMSEKDFEE
                     TRSFLKSYTRLYAQTPARRLAYMMDSRFYGRQDWLNELGKLLEKATREDVNAAIREFW
                     QTKNMHIAIITEPKESRAIQNALAEQRSTPVSYSNTLSATLPASILEEDKLVEKYPLP
                     PATVEVVTSDVPFQ"
     gene            complement(7679..8188)
                     /locus_tag="P5292_00415"
     CDS             complement(7679..8188)
                     /locus_tag="P5292_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRS37616.1"
                     /translation="MLSKKINTLLNKQVELEASSSNVYLAMASWAEVQGFEGVSNFLY
                     KHSDEERMHMLKLMKFINERGGHALVPTLKAVQSSYKNVQQLFEQILEHEVGVSAEIN
                     KLVDECLKEKDYTTHNFLQWYVSEQIEEERLARTVLDKLKMIGGDKSGMYLFDRDLSQ
                     LSVAEEGNG"
     gene            8363..>9288
                     /locus_tag="P5292_00420"
     CDS             8363..>9288
                     /locus_tag="P5292_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRS37617.1"
                     /translation="MPAASGNKSRLVLLIWIVVLGPLLLLSGLMFFAYKGWVGEQLPS
                     FEELENPKSALASEIISSDQQLLGKYFIENRTNAHYYELSPNVIQALKATEDIRFEEH
                     SGVDLRGLLRAVFRAGTAGGGSTITQQLAKNLFHEKPGNKIERVIQKIQEWIIAVRLE
                     RRYTKEEIMAMYLNTVEFSSNAFGIKSAARTYFGKGPDSLNVQESALLVGMLQAPTRY
                     NPVRNPKNSFARRNVVLGQMKKYDFLTEEAFDSISALPIRLNYHQEDHNFGLATYFRE
                     VMREELLKWCKEHINNSTGKPYSLYTDGLRIYT"
BASE COUNT         2191 a         2666 c         2529 g         1902 t
ORIGIN      
        1 ccggtggagg ccggccggcg tgacccgcgc aaaggcccag aggtaaggcc cgccggccgt
       61 caccgcgtcg aatgcaaggc cgtagatgcc ggccagtccg tgtacggcag acggaatggt
      121 ctgcaaggtc gcgccagtcc ggtcgaagag caccatggga taatcgtcgt atgccaccca
      181 gaacgcgtcg cgggtggaat catacgcgac gcctttcggg aacaagggca gcagaatgga
      241 atccgtaacg gctaaggtgg aagggttgac ttcgtagagg tagatgccct ggtcctgcgc
      301 gatgtagaga ctactgccat ccgtcgtgat cgcggtggta ccggtgtcgg gcaagccggt
      361 aatggtgagc gaatccacca ggctgccacc gacggaaaag cggcggatca tgggcccgtc
      421 gaatccgccg gtccagaatc gcccctggaa gtagagcgcg caggtgtaag catcgttacc
      481 ggaaggtgca ctgaggttga agccggactg cgggtcgaac aggcgagccg aagatggctc
      541 ggtgtgaaaa ccggacatcc tgaagcgaag gtcctggtcg gattgcgcag cgagccggag
      601 tgtgatgccc gcaatcagga agagtgtcaa acgaaggttc atgatgggtg tgaattcggt
      661 taaagcgatc gaaagttaaa ataaaaccgc agctcacaag cgtacccgaa ttgtcataat
      721 agcgaaccgg ccttcatcct ccttccgaca aagaatacgc gcctttcaac aatcacttgc
      781 tggaaaaccg ggcccggctt ccggccggaa agctgaagca tttctttctt tcgcaacatc
      841 atccaacctt tcaccaaacc cacgcaccat gcatcgtcct ttacattcac tttcgttcgt
      901 actgctgttc gtcgctcttc agttgtccgt tactgcacaa caggtatgcg actccgtttt
      961 cacaccctgc ttcaatacga cttccggcac cggcttcttc ctcgacctgg aagcctcgac
     1021 cgatgtaacc attgagggct tctccaccat gtcgcaggga gccggaaccc gtgatgtgga
     1081 attgtactat cgtcccggtg gttttgtcgg atacgaaacc gacgccgctg cctggacgct
     1141 cgccggcagt gccagcggct ttgatcctgt gagtgccatt tcatgtccca ttccggtgac
     1201 accgctgcca atcaccttca acgtctgtat cccggcagga gagcgctacg gtttttacct
     1261 cgccagcacg agcggttccg gttcctttga actgtacgat acgctgacga ccgggaccct
     1321 gttctcggac gacggtacgc tgcgtctgta cgtcggcaaa ggcgcctttg ctttcggagc
     1381 attcaccgga tttgtcacgc agaacaaact catgcagggt acgatccagt attcctgcgg
     1441 ctgctcgacc ggaatgcagc atccatcggc tgcatcgaac gtgcgcttgt tcccttccat
     1501 cgtgacgcgc gaattccagc ttgacctttc caaggcacag ggtgtttatc aatccgcacg
     1561 catactggac agtgccggtc gactgctcga tcagctcgaa ctggacgcca ccgcgaccag
     1621 cattatccga aacgtcgctt cgctttcgcc gggtgtgtac accttgcaat tgaccggttt
     1681 gaaatcctcc gcgcagctca aattcgtgcg gagcagcgat taatcggtca cccaaacacg
     1741 acgttcaagc gtgcagggaa tgcagtggat gcgtacgcgt ggataccatt gctttacctt
     1801 atcatggtca tgtatctgac gatcagcctc ttagcattgg cgaaaattca ctatttttga
     1861 acctcatcag aatttcctcc cctatttatt atggcttgcg cttcgtgcgg aacaggaaca
     1921 gacgggaaac cgggtgggtg ccgcagcaac ggcacctgta gcacaggtgg ctgtaacaag
     1981 ttgaacgtct acaattggtt gtcggatatg gtgttgccca acgggcagca gtcgttcgac
     2041 gttgtagaag tacgcttcaa aggcagccgc aaagaatttt tccggaacaa agagctgcag
     2101 gacctgcgtg tgggtgatat ggtagcggtg gaaggaagtc ccggtcacga cgtcggcacg
     2161 gtgtcgatgg ttggcgagtt ggtgcgcttc cagttgcgca agaagagtct ggcagagaat
     2221 tcggagtcga tcaaagcgct ctaccgcaaa gccaagccgg gcgatgtgga gaagtggtgc
     2281 gagatgaaag acaaagagcc cgccacgcgc aagcgcacgc gggagatcat cgaatcgctc
     2341 aagctcaaca tgaagttgag cgatgtcgaa ttccagggcg acggcaagaa agccaccttc
     2401 tactataccg ctgaagagcg cgtcgatttt cgagaactca tcaaacgtct cgccgatgag
     2461 ttccgtatcc gggtcgagat gcggcagatc ggcatgcggc aggaagccag ccgattgggc
     2521 ggcattggtg tttgtggccg tgaactttgc tgttccacct ggctcacaga tttcaagacc
     2581 gtatcgacca gcgcggcgcg ctatcagaat cttgcgctca atcccgccaa gctcgcggga
     2641 caatgcggca agctcaagtg ttgtcttaac tacgaactgg attcgtatat ggacgcggta
     2701 cgcgatattc cggcttcgaa catcatcctc gaaaccgaga aaggtcgcgc gttccaccgc
     2761 aagaccgata tcttcaagcg attgatgtgg tacgcgttca gcgagaacga gcggagaggc
     2821 gaaggcgaac aagcgttcac cggcgaaggc aactgggtcg tcatgcccgt cgatcgtgtg
     2881 aaggagatcg tcgcgctgaa caaagaaggc aagaagccgg gcgaactcat ggaaggcgac
     2941 tggctggaag tggaggtcga agaagaaccg gactacgcca acgtggttgg tcaggaccgg
     3001 atcgaccgga tggacaagaa gaagaagaaa aagaagaaga agaaatcatc cggcagcggc
     3061 aacgctcaac aggcagctcc aacgggtcag gcacagggcg gacaacccca gggtggtcaa
     3121 ccgaaagaac gcggacagca aggtggtcaa caacggcagc gacccgacaa ccgtccgcaa
     3181 ggcggcaaag gaggcgggcc tcgtccgcag cagaaccagc agcaaggcgg caaaccgcag
     3241 cagcagggtc agcaacagaa ccaacagcaa cgtcagcaac agggacagcg cccccagcag
     3301 caaggacaaa acaacaaccg tcagcagcaa cagggaccgc gtccgcaaca gcaacaaact
     3361 ccgcgtccgc agggcgaaca gcgtccgcag caggagcagc gcccacaggg cggaaaccgg
     3421 gagccacgtc agcggccttc cgcacctccg cgtcagtcgc ccggacaatc aacgccgccg
     3481 ccagaatcga actgatccgt gaacaaacct tcacttcgta ctctcctgct gcttacgatc
     3541 ctgtcgttgt cgctgagttc gtgcgaccgt tccgtactgt tcgaagagaa cgttcgcatt
     3601 cccgacaacc gctgggatgc cgcgaacgtc atcaagcttg aggcggatat tcccgatacg
     3661 gctcagttgt acaatctcta cgtgaacgtg cgcaatgccg gcgggtatcc ctactccaac
     3721 atcttccttt tcctcaacac caccctaccc gacggcaagc gttcacgcga taccctcgaa
     3781 gtgaccctgg ccgacgacaa aggacaatgg ctgggtgacg gcatgggcga tatctgggac
     3841 aaccgcgcac tcttccgcga gcggatgcgc ttcccacaga gcggccttta cacgttcgag
     3901 atcgagcagg cgatgcgaca gaatcccctt ccgcagatca tggatgtcgg cttgcgcatc
     3961 gagcgatacg acgatccggc cgggcgttga tcctgcttcg cctcaggtta atctccgccc
     4021 gctgcttttt ctcaatcgga gaatgatggc tccttccgcc aacgcgagca aggcgaagaa
     4081 ccacagccac gcgcttcgac cgctccttcc ggatttatac ttcgtcagct ccgcctcgta
     4141 acgcgcggca cgggccgtca tcgtgtcgga tacggcctgt aggttccgga tacggtccgc
     4201 gtcgcgggag gcatcgtagg tcatgctgac ccgttcgaag gtgcgctggt ccagcatacg
     4261 atcgagcagc cggatgatct cgttgtcggt ctctacgatc gctgccagac gatcgtgcga
     4321 acctttcagg tcggtcttgg tacgctggcc gaagattccg cttttttgct gcaggctgcc
     4381 cgtgtattcc cggaattgcg cagcgcgacc ttcgagcagg tggatcagtt cggctcgttc
     4441 ggcggagttt ccgttcgcaa aggaattcag ggaatagcaa aagcaggcaa ggaccaggaa
     4501 gtgtcgtagc atcgtaacca ttaacgtaat ttaagaatcc ttattggtcc tgcgatgaaa
     4561 cgggtaccgc cttttcgata ccttcgcagg acacgcatct ttatcaagat cctacatgag
     4621 aagaatcatc ctgctgctgt gttgcagcat tgccctcctg gcgcaggcgc aagcgcccaa
     4681 gaagaaaccg gttcccgcgc aaccggcttc cccgaagccc ggccccgccc agacaaaagc
     4741 gatcaacgct cccgcgccgg cacccgacat taagaacaag gtgctgccct acaccattca
     4801 ccagaaaaaa atggccaacg ggctcaatgt ggtaacgatc ccgtttgaaa gtccgggcgt
     4861 tgcggcgttc tacatcgtcg tacgcgtagg ttcgcgtgac gagatcgaac cgggaaagac
     4921 gggctttgct cacttcttcg aacacatgat gttccgtggc acgaagaagt atccgaaaga
     4981 agcatacagc gaggcgctga aaatgaccgg cgcttccgcc aacgcgaata ccagcctcga
     5041 ccgtacggtt tatcacatga ccggtgatgc ctccaaactc gagaagatgt ttgagttgga
     5101 agcggaccga ttcatgaacc tgagttattc ggaacaggat ttccggacag aggccggagc
     5161 ggtgaaaggc gagtacacca agaactcctc cagtccctac acacggctga acgagaaatt
     5221 gcaggagacc gccttcgacc agcacaccta caagcatacc accatgggtt tcctgaagga
     5281 catcgtggat atgcccaatc agttcgccta ttcccgccag ttctttcaac gctactaccg
     5341 acccgaatac accaccatcc tggtcgtggg cgatgtaacg ccggcaggtg tcgaagcgct
     5401 gtcgcagcgt tacttcggta cctggcagcg cggcacgcac gttaacaaga tcccgtccga
     5461 acctgcacag aaaggtaccc gctacgcgca catccagaag agcggattcc ctccgtacct
     5521 cagcctgaat ttccgtggcc ccggttactc cgacagtcag aaggacgtcg cggcactgaa
     5581 catccttggt tcggccctgt tcggtgaaaa ctcgcccttg tatgaaaagt tggtgatcaa
     5641 cgagcagaag tgtcgggaaa tcgacggagg catctttcct acgcgagatc cttttctctt
     5701 cagcgccgac gcttcgctgg tgaatgcagc cgatatgaag tatgtacagg agcagatcaa
     5761 cctggcgctt gccggggcga aagaagctcc gatggatccc gcacggatcg atcaggcacg
     5821 ccaaaacatc cgcaactcgt tcgccatgcg gatcgacaac cccacttcga ttgccgaagc
     5881 ggttagcaac ttcatctggc tgacgggaga tcccgaatcg atcaaccggt attacgacca
     5941 atacgaacag gtgacgccgt atgacgttca gcgtgtcgcc cagcagtatt gcgtttccga
     6001 gcagtgtacc attggtacga tttccccttc cgaaaaatcc gatctacaat gaaccgccac
     6061 ttcacccaac cgatgaatat ccgcatcctg gccctgctgc ttaccctgag ttgctgcgtt
     6121 tcgaccgccc gcgcgtttga aacgattgaa ctccgtcagc ccggcaccaa taagatcgcc
     6181 atccggctga tgttccggaa cggctccgcc tgcgacccgg aaggcaaaga agggttgatg
     6241 tgcctgacag ccagccttct caccgaaggc ggcacggaat cgatgtcggc cgccgatatc
     6301 accaaaacac tttatccgat ggcggtccgc atgggcagtt ccatcgataa ggaagtctcc
     6361 atcgtttcgt tcgagttcct caaggaacac ttcgagcgct tctacccgat tttacgcgac
     6421 atggtactgg cgccgggatt ccgccaggaa gacatcgacc gcgtttcgtc gaaccagttg
     6481 aattatctca ccgatgtcat ccgtcaatcc tccgacgaag aattcggcaa gaagtggctc
     6541 gaagcgagac tgttttccgg aacacgctac gcgcacctgg tagagggcag cgttaccggt
     6601 ttgaagggca tcacacgcgg cgatatcacg gactgctaca agacctactt cacccgcggc
     6661 aatttattgc tggggatcgc cggcgactat acgaccgaac agctttcact cctgcaatcc
     6721 gacttgcaga agctcccgga cactccggtc aagtgtaact tcggatccgt aacggctccc
     6781 tccggttttc aggtgagcat cgttgagaag gaaggagcca tgggttcggc catttccgcc
     6841 ggattcccga tgagtctgac ccggtccagc gaccaattcg cggccttgat gatcgccaat
     6901 tcctggctag gcgaacaccg taaatcgtat tcccgtcttt accagaaaat ccgggaagcc
     6961 cggtcgatga attatggcga ctacacgtac atcgaatggt atgagaatgg cggttccaac
     7021 atgttgcccc ctccgggcac gccgcgcagc atgaactact tctcgatctg gctgagaccg
     7081 gtacagacgg tcacgagtct gaagaaccaa tacccggaac tcaaggacat cgaaatcggt
     7141 cattccctct tcgctttgcg catggccatc cgggagatcg atcaactgat caaaaccggc
     7201 atgtcggaaa aggattttga agaaacccgt tcgtttctga aaagctacac gcgcctgtat
     7261 gcacaaacgc ccgcacgccg gcttgcctac atgatggatt cgcgtttcta tggccgtcag
     7321 gactggctga acgaattggg caagctgttg gaaaaagcca cgcgcgaaga tgtgaatgcg
     7381 gcgatccggg aattctggca gacgaagaac atgcacatcg cgatcatcac cgagccgaaa
     7441 gaatcacgcg ccatccagaa cgccctggcg gaacagcgct caacgccggt atcctattcc
     7501 aataccctgt cggctaccct tcctgcatcc attctggaag aggataaact ggtcgagaaa
     7561 tacccgcttc ctccggcgac cgtggaagtc gtgacttcag acgtaccctt tcaatgatcg
     7621 atcggggact gaaacaaaaa aggcgaccgt atggtcgcct tttttgttga cggctgaatc
     7681 aaccgttacc ttcttcggcc accgaaagct ggctgaggtc acgatcgaac aggtacatgc
     7741 cgctcttgtc gccgccgatc atcttcagct tgtcgagcac cgtacgcgca aggcgctctt
     7801 cctcgatctg ttcggataca taccactgga ggaagttgtg cgtggtgtag tctttttcct
     7861 tcaggcactc atcgaccaac ttgttgatct cggccgaaac gccgacctcg tgctcgagga
     7921 tctgctcgaa caactgttgt acgttcttat aggaagattg aacggctttg agggtgggca
     7981 ccagcgcgtg tccaccacgc tcgttgatga acttcatcaa cttgagcatg tgcatccgct
     8041 cttcgtcgga atgcttatac aggaagttgc tcacgccttc gaatccctgt acctccgccc
     8101 aggaagccat ggcgaggtaa acattggagg aggaagcctc cagttcgacc tgcttgttca
     8161 acagggtatt gatcttcttg gaaagcatat tgtcggttct gtttttctca gcaacaaaat
     8221 tagtcgaaaa atagtttacc cggtggcgct tacccggcgg gacgcaatga aatgttggtt
     8281 cttccgttga atactcggcg atcccggagg ggctgccccc ttcgaaatcc gtaatttgga
     8341 acccttattc cccagatccc acatgcctgc cgcctccggt aataaatcac gcctcgtcct
     8401 gcttatctgg atcgtggtcc tcggcccctt gctcctgcta tccggcctga tgttctttgc
     8461 ctacaaaggg tgggtcggag aacagttgcc cagctttgag gaactggaaa acccgaagag
     8521 cgccctggcc tcggagatca tctcttccga ccagcaattg ctgggaaagt atttcattga
     8581 aaaccgtacc aacgcgcact actacgaact gtcgcccaac gtcatccagg cgctcaaagc
     8641 cacggaggat atccgttttg aggagcatag cggcgtggat ctgcgcggtt tgttacgggc
     8701 ggtatttcgc gcaggtaccg ctggtggcgg cagtaccatc actcagcaat tggcaaagaa
     8761 tctcttccac gaaaaacccg gcaataagat cgagcgggtt atccagaaga tacaggaatg
     8821 gatcattgcc gtacgcctcg aacgccggta caccaaggaa gagatcatgg ccatgtacct
     8881 gaacacggtc gaattcagca gcaacgcctt tggtatcaaa tccgctgccc ggacttattt
     8941 cggaaagggt ccggacagcc tgaatgtgca ggagtcggca ttgctggtag gcatgttaca
     9001 agccccgaca cggtacaacc ctgtacggaa cccgaagaac agctttgccc gccgaaacgt
     9061 cgtattggga cagatgaaga agtacgactt tctcaccgag gaggcgttcg actcgatcag
     9121 cgccttgccc atccggctga actaccatca ggaggaccac aacttcggac ttgccactta
     9181 cttccgggaa gtgatgcggg aagaattgtt gaagtggtgc aaagagcata tcaacaattc
     9241 aaccggtaag ccctatagcc tctatactga cggtctgcgt atatacac
//
LOCUS       DAOTON010000009         9208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_6875_length_9208_cov_3.805528, whole genome shotgun sequence.
ACCESSION   DAOTON010000009 DAOTON010000000
VERSION     DAOTON010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6875_length_9208_cov_3.805528"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1791
                     /gene="hrpB"
                     /locus_tag="P5292_00425"
     CDS             <1..1791
                     /gene="hrpB"
                     /locus_tag="P5292_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HRS37618.1"
                     /translation="AALLEEHGPGGIRVYSLYGDLPHEEQQRALQPDPNGLRKVVLST
                     SIAETSLTIEGIRVVVDSGYARVPRFDPRSGFTKLETVSATSDTTDQRAGRAGRLGPG
                     VCYRLWAEHAQGHRVPHRKPEILDADLAQLVLELAAWGHHDLSALTWLTPPPPGSVAQ
                     ARNLLESLDALESGKITDAGKRLLKFPTHPRLAHLLLYGQEHGLATLAADAAAVIEER
                     DPLGKEAGADLSLRVETLRRWRKKERVAADRAALDRIDRVARQWCRLLHMQTETGAFE
                     HHDLGRLVAAAYPERIGRRDDERGRYRLANGRAARLPEHDPLTHDAWIAVAQLDAGTN
                     EGKIFLAAPYDPSDALADARPQAVIEWDERNGQLIAREEWRLGNLVAATKPLTNPKPE
                     QVAAAVLTTLRTNGLRLFEPNETVLQFIARAGALANWRPELDLPDFSEAGLLASLEEW
                     AAPYLGEIRKREDLRKVPVYDLLKNRLSWEQQSILDRLVPERIEVPSGSQIRIEYFAD
                     GAPPVLAVRLQEVFGWTDTPTVNEGRTQLLMHLLSPGYKPVQVTRDLRSFWQGTYHEV
                     RKELRMRYPKHSWPEDPWTAQAVRGVKKRN"
     gene            1869..2369
                     /locus_tag="P5292_00430"
     CDS             1869..2369
                     /locus_tag="P5292_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HRS37619.1"
                     /translation="MESHWQYIFISALIPMFTGFIWYGPKTFGNVWMREAGVSEEKIK
                     SSNMALILSLTYVFSLFLSLILISVVIHQSHILSILADDPGMKDPNSEISLWLKNFMD
                     QYGQNFRTFKHGAFHGTLSGIFYAFPIIAINALFERRSWKYIWIHTGYWILTLALMGG
                     IVCANF"
     gene            2502..3347
                     /locus_tag="P5292_00435"
     CDS             2502..3347
                     /locus_tag="P5292_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37620.1"
                     /translation="MRKFLLLSLCTIATLTVFAQPQYFKGLGTSNSTFLLGNTSYASR
                     SQMIFLPSDLGNPPAGTIQRIYFRYGSTGQATGNTLTDFEVRLLQTTAIAFPNTDNFF
                     TGLTTVLSSPSYVIAPGTTGDWFAIELTNPFAYDPSQNLVVHTQMSASANQVFGTYGT
                     NNTGQKLVSPNLTDTVGSLTSSTWQDFGFDFLLNGLDPSAAKLPAVSLYPNPTSNLLT
                     VTIDGPSVSGNLVVRDMQGREMLRRRIEESGKTVINTGDWSTGSYWLIVETPEGHAYR
                     QFLRQ"
     gene            3429..5558
                     /locus_tag="P5292_00440"
     CDS             3429..5558
                     /locus_tag="P5292_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRS37621.1"
                     /translation="MKKILPFLLFALTIGFSVRAQVSTISPASANPGQSLTTTITLAT
                     GVMNSGSPPQSNVDIYLQQGAYQIFTDYFDETQVYPDGFGGYSDSLFTNFSIPANAPL
                     GWYEIHVVTYTPWDPWFGQQPVDNMLDYGFVVADPNSCPVPSGVSTSNVTATTIDINW
                     TDPTVADTFRIRYQPVGATTYQYLDVQGSGGLTSAPLSNLQPGTLYSYEIATRCNGYP
                     STYSIIDYFITDVAIVNCVRPFGLAITSVNNIDATIEWSPFLAADTFRIRYRPVGGSH
                     FSYINHDGGLGNSELLDNLYPGTDYEVQVSSICNGVSSGYCPKEYFTTLSNPVSCVAP
                     LGASAGSITATSAVISWSSLILADTFRVRYREFGALNYNYIDLDGATGDTSVSLTGLN
                     PNTTYYYQVSSICGGVSYGYCSQSNFTTLNQTINCGRPFNVSVGSITTGSAVVSWDPL
                     TVADTFRIRYSIDGTTNYSYIEHDGSGPNSQTISNLLPNTTYQVRVSSICNGVSSGYC
                     RPQVTFTTLNGPVSCVTPYGLGHSNVTGTTADISWTPLVQADSFMIRYSVNGTTNYRW
                     KKITGAGGVTSTTLTGLSTSTDYQWQVRSICSGVTVSIYSASDVFTTAPVRLANPETV
                     VASDWQLYPNPATRNVNLNVEATTAGTQTVRVTDLLGNTVITRNFPVESGNNQLQLDL
                     AGLSPGVYFVTLIADNRELRGKRLIVN"
     assembly_gap    5799..5898
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6095..7768)
                     /locus_tag="P5292_00445"
     CDS             complement(6095..7768)
                     /locus_tag="P5292_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS37622.1"
                     /translation="MNQSTTKTDWSKCSLFQHCSQAVIDEVVAKASAKVYKKGGVLID
                     KGQVPPALFVIEDGKVGIYIEDILLAELGAMAVMGESFLAHGVATATIVAASDVKTIE
                     IPRQLFTELAEQYPQLIFNIFTINFERLRSSNDAALREARAREQKLEEQVKERTRELN
                     AALEELQVTNQELTTTRDNLIETQKFRDQFLANMSHEIRTPMNAIVGLTNLLINSPLN
                     PLQEKYLHVIKKSGANLLVIINDILDLAKLEAGKMELEAVPFPLVTAIQNVHTILNLK
                     AEEKGITLVESIDANVPFYVVGDETRITQVLMNLTGNAIKFTEKGKVTIGAKVTAGTD
                     DEPEITFSVRDTGIGIPPDRLDKIFESFGQASSDTTRKYGGTGLGLTISKQLVEMHGG
                     QLEVKSKVGEGSVFFFTVKYKRAEPPSDAIRESSQAGIDMSGKRILLVEDNEFNQMVA
                     VDTLQELFPGIVVEVASNGKEAMQMAADFDYGLVFMDIHLPDFDGFEITQYIRNKLTG
                     PRQAVRICAMTASVTKEKIDECYVAGMNDYLMKPFTHEGLKERIYRAFA"
     gene            complement(7838..>9208)
                     /locus_tag="P5292_00450"
     CDS             complement(7838..>9208)
                     /locus_tag="P5292_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37623.1"
                     /translation="IVWSRVYGTAGYDNSEYAVVAADGGIVCTGRLNITGGNEDALVF
                     KTDSLGYLLWAKAYGGAAADGFAYVAEAGNGDLLFVGATQSVPGTLNSLFLMRTDANG
                     DTIFTKVFDTASRDIGLCVAETADSGLVVAGKLVYNNWAGLEQSNALVMKTDRNGNLL
                     WSRMYGDTLWEELASIVPLADGGFIAAGTSVGNGPGRYECWLLRLDSIGDVLWSKTYG
                     GIGDEAAYTVIADPDGGFVLSGYSNSLGYGHRTAGDDAMNVVLIKTDANGDTLWCRAY
                     GDGLQDEAYRSSKADDGGYLISGFTTSYTPANLSQMLLIHTDSLGWTGCHEQAIQPVV
                     TTATYVPTDTSMILYSGMTVSNPGYLEATATPVSDDACLILATNEFDLITGIRLFPNP
                     AADLVNVEVPMASAAETVELLDLSGRLLQRERIFGGKARLDLSAFSAGCYLLRAGSLT
                     GRLVKN"
BASE COUNT         1925 a         2895 c         2333 g         1955 t
ORIGIN      
        1 gccgctctgc tggaggagca tggcccgggc ggcattcggg tgtattcgct ctacggcgac
       61 ctgccgcacg aagaacaaca acgcgcgctg caacccgatc cgaacggctt gcgaaaagtc
      121 gtgctatcga cttccatcgc agagacatcc ctcaccatcg aaggcatccg cgtcgtcgtc
      181 gacagcggct acgcccgcgt gccgcgtttt gatccgcgct ccggattcac caaactggaa
      241 accgtcagcg cgaccagcga caccaccgat caacgggccg gtcgtgccgg tcgtctcgga
      301 cccggtgttt gttaccggct gtgggccgag cacgcgcaag gccatcgcgt accacaccga
      361 aagcccgaga tcctcgacgc agatctcgcg caactcgtct tggaacttgc agcctgggga
      421 caccacgacc tctcggcgct gacctggctg acaccgccac caccaggctc tgtggcacaa
      481 gcccgcaacc tgctcgaatc acttgacgcc ctggaatccg gtaagatcac cgatgcagga
      541 aagcgcctct tgaaattccc cactcatccg cggctcgcgc atttactttt gtacggacag
      601 gagcatgggc ttgccacact cgcggccgat gcggcggccg tcatagagga gcgcgatcca
      661 ctcgggaaag aagccggtgc cgatctgtcc ctgcgtgttg aaaccctgcg tcgctggcga
      721 aagaaagaac gcgtggctgc cgatcgcgcc gcgctcgacc gcatcgaccg cgtggcgcgg
      781 caatggtgca ggttgttgca catgcaaacg gaaaccggcg ccttcgaaca ccacgacctc
      841 ggcagactcg tcgcggccgc ctacccggag cggatcggtc ggcgcgacga cgagcgcgga
      901 cggtaccgtt tggcaaacgg tcgcgccgcg cgtttacccg aacacgaccc gctgacccac
      961 gatgcctgga tcgccgttgc gcaactggat gccggcacga acgaaggaaa gatctttctc
     1021 gctgcgccct acgatccttc cgatgcactg gccgatgctc gtccgcaggc ggtcatcgaa
     1081 tgggatgaac gcaacgggca actgatcgcg cgggaagagt ggcgcctcgg caatctggtc
     1141 gccgccacca agccgctcac gaatcccaaa ccggagcagg tagccgccgc ggtcctgacg
     1201 acacttcgaa cgaatggact ccgcttgttc gaaccgaatg aaacggtctt gcaattcatc
     1261 gcacgcgccg gcgctcttgc caattggcgg cccgaactgg acctccctga tttttccgaa
     1321 gcgggactac tcgcatcgtt ggaagaatgg gcagcgcctt atctcggaga aatacgaaag
     1381 cgggaagact tgcgaaaagt tcccgtgtac gatctgctga agaaccggct gagctgggag
     1441 caacaatcca tactcgatcg cctggtgccg gagcgcatcg aagtaccgag cggatcccag
     1501 atccggatcg aatacttcgc cgatggcgcg ccgccggtgt tggcggtccg tttgcaggaa
     1561 gtgttcggct ggaccgacac accgaccgtc aacgaaggac ggacgcaact gctgatgcac
     1621 ttgctttcac cgggatacaa accggtacag gttacgcgcg acctgcgcag cttttggcaa
     1681 gggacgtatc acgaagtgcg aaaggaatta cgcatgcgct atccgaaaca ttcctggccg
     1741 gaagatccct ggaccgcgca ggcggtcagg ggcgtaaaaa agcgcaatta acagtcatcc
     1801 cgttcctgat ttggagactg tgcaggggat tcgttacttg actgcccaaa ccctaaaccc
     1861 tccccactat ggaatcacac tggcagtaca ttttcatcag cgcgctcatt ccgatgttca
     1921 caggatttat ctggtacggt ccaaaaacat tcggtaacgt ctggatgcgg gaagccggcg
     1981 tcagcgagga aaagatcaag agctcgaaca tggccctcat cctcagtctg acctatgtgt
     2041 tcagtttgtt cctctccctc attctcattt cggttgtgat ccatcaatcg catatcctct
     2101 ccatcctggc agatgatccc ggcatgaaag atccgaactc cgagatctcg ctttggctca
     2161 agaacttcat ggatcagtac ggtcagaatt tccggacctt caagcacggc gccttccacg
     2221 gcaccctgtc gggcatcttc tacgcgtttc cgatcatcgc gatcaacgcg ctcttcgaac
     2281 gccggagctg gaaatacatc tggatccata ccggttactg gatcctcacc ctcgcgctca
     2341 tgggcggcat tgtctgtgcg aacttctaaa catccacagc ttgttatcgg aaaaggggcg
     2401 aaaatttcgc cccttttttc atgcgtaccg gtggaaaatt acgggcatca accggactga
     2461 gggatacaca tcctgagtag ctttgagaaa agctccttcg catgcgcaaa ttcctactcc
     2521 tgtccctttg cacgatagct accctgaccg tattcgcaca accgcagtac ttcaaaggtt
     2581 tgggtaccag caacagcacc ttcctgctcg gcaacacttc ctatgcaagc agaagccaga
     2641 tgatttttct gccgtcggac cttggaaacc ctccggccgg aaccattcag cggatctatt
     2701 tccgctacgg cagcacaggc caggctacgg gtaatacact caccgatttc gaagtgcgcc
     2761 tgctgcagac cactgcgatc gcgtttccaa acaccgacaa ctttttcacc ggcttaacga
     2821 ccgtgctgtc atccccttcc tacgtcatcg cacccggaac gaccggtgat tggttcgcca
     2881 tcgaactgac caatccgttc gcgtacgatc cttcacagaa cctggtcgtt cacacgcaaa
     2941 tgtccgcttc agccaaccag gtgttcggca catacggcac caacaacacc ggacaaaaac
     3001 tggtatcgcc gaacctcacc gatacagtcg gcagcctcac cagttccacc tggcaggatt
     3061 tcggcttcga cttcctgctg aacggacttg atccttcggc tgcgaaattg cccgcggtct
     3121 ccctgtatcc gaacccgacc agcaacctgc tgaccgtaac aatcgacgga ccatcggtct
     3181 cgggcaacct ggtcgtacgc gatatgcagg gacgggaaat gctgcgcaga cggatcgaag
     3241 agtccggaaa aacggtgatc aacaccggcg actggagcac gggcagctat tggctgatcg
     3301 tggaaacccc ggaaggtcac gcttatcgtc aattcctccg gcagtaagtt attcttcaaa
     3361 ttatccgcct ttataaaggc aaccctttcc atccgatctc gtacacgaat agattattcg
     3421 ctccgcccat gaaaaaaatt ttacccttcc tccttttcgc gctaaccatc ggcttctcgg
     3481 tcagggcgca ggtcagtacg atctcgcccg cctccgccaa ccctggtcag tcgcttacta
     3541 ccacgatcac actggccacc ggtgtgatga actcgggctc accgcctcaa tccaacgtcg
     3601 atatctacct ccagcagggt gcatatcaga tcttcaccga ctacttcgac gaaacccagg
     3661 tgtatcccga cggtttcggc ggctattccg actccctgtt caccaatttc tccattccgg
     3721 ccaacgctcc gctcggctgg tatgagatcc acgtcgtgac ctacacgccc tgggatccct
     3781 ggtttggcca gcagccggtc gataacatgc tcgactacgg cttcgtggtt gccgacccga
     3841 atagctgccc ggttccttcc ggtgtttcta cctcgaacgt caccgccacc accatcgata
     3901 tcaactggac ggatccgacc gtagccgata cgttccgcat ccgttaccag ccagtcggcg
     3961 cgacgaccta tcaatacctt gatgtacaag gcagcggtgg actcacttcc gcgcccctca
     4021 gcaacctgca acccggcacc ttatatagct acgagatcgc gacacgttgc aatggttatc
     4081 ccagcaccta cagcatcatc gattatttca tcaccgatgt agccatcgtc aactgcgtgc
     4141 gtccattcgg cctggcgatt acgtccgtga acaacatcga cgcaacgatc gaatggtcac
     4201 ccttcctggc agccgacacg ttccggatcc gctatcgtcc tgtcggcggc agtcacttct
     4261 cgtacatcaa ccacgacgga ggtctcggga attccgagtt gcttgacaac ctctatcccg
     4321 gaaccgacta cgaagttcag gtcagttcca tctgcaatgg cgtgagcagc ggctattgtc
     4381 cgaaagaata tttcacgacc ctgagcaatc ctgtatcctg cgttgctccg ctcggcgcca
     4441 gcgccggcag catcacggcc accagcgcgg tcatcagttg gtcgtcgctc atcctggcgg
     4501 atacattccg ggtgcgctac cgcgagttcg gcgcgttgaa ttataattac atcgacctcg
     4561 atggcgctac cggagacacc tcagtcagcc tcaccggcct gaacccgaat acgacgtact
     4621 attaccaggt gagctcgatc tgtggcggcg tcagctacgg ttattgcagc cagtccaact
     4681 tcaccacgct gaaccagacg atcaactgcg gtcgcccctt caacgtcagc gtcggatcga
     4741 tcacgaccgg ctccgcggta gtaagctggg atccgctgac cgtagccgat acgttccgca
     4801 tccgctactc gatcgacggt accaccaact acagctatat cgaacacgac ggcagtggac
     4861 ccaattcgca gacgatatcg aacctgctgc ccaacacaac ctatcaggtc cgcgtcagtt
     4921 cgatctgcaa cggcgttagc tcgggttact gtcgtccgca ggtgaccttt accacactca
     4981 atggtcccgt ctcctgtgtc acaccctacg ggctcggaca cagcaacgtc accggtacga
     5041 cggccgatat ctcctggacg ccgctggtac aagccgacag tttcatgatc cgctattcgg
     5101 tgaacggcac caccaattac cgctggaaga agatcaccgg cgccggcggt gtgaccagca
     5161 ccacgctgac gggtctctcg accagcaccg attaccagtg gcaggtgcgt tccatctgct
     5221 cgggcgtgac cgtttcgatc tactcggctt ccgatgtctt taccaccgcg ccggttcgtc
     5281 tcgcgaatcc ggaaacggtt gttgcaagcg actggcagtt gtaccccaac cccgccacgc
     5341 gcaacgtcaa cctgaatgtt gaagcaacaa cggcaggtac acagaccgtc cgggtaaccg
     5401 acctgctcgg caataccgtc atcacacgga acttcccggt tgaatccggc aacaaccaac
     5461 tccagctcga cctggcagga ctgagtcccg gcgtttactt cgtcaccctc atcgccgaca
     5521 accgggaact ccgcggcaag cggttgatcg ttaactgata acttacgcgg aacacgtttg
     5581 ctgttccggg tttcgggttc cgggttccga gttcatcggg acccggaatt tttttagggt
     5641 ctagtagttg cgaaaaccgg aatggaccaa cggttcaatg aagtggtaac tggtaattgg
     5701 ttattagtaa ttagtgattg gtgattcgcg aggtagatct ttcttctctc gctttcgcct
     5761 ctccgctcaa cagtcgcatc gctcgaaaag gccgtcgann nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnntt ctctcgcttt cgcctctccg ctcaacagtc gcatcgctcg
     5941 aaaaggccgt cgatctcatc cccttccctc ggtcacggtc tcgtaactcg cgtctcaatt
     6001 ccctcgcccc tcgtccctat aaaaaaggcc ggcccctttc ggaaccggct ttgctgagtg
     6061 gtgtgtgtgt gtgtaacctc agctcgtact cttgtcaggc aaacgcccgg tagatgcgtt
     6121 ctttcaatcc ttcgtgcgtg aagggcttca tcaggtagtc gttcatgccc gccacgtaac
     6181 actcgtcgat cttctctttc gtcacgctcg cggtcatcgc gcagatgcgc accgcctgac
     6241 gcgggccggt gagtttgttg cggatgtatt gcgtgatctc gaagccgtcg aaatccggca
     6301 agtgaatgtc catgaacacc agtccgtagt cgaagtcggc ggccatctgc atggcttctt
     6361 tcccattcga agcgacttcc accacgatac ccggaaacaa ctcctgcaag gtatccacgg
     6421 caaccatctg gttgaactcg ttgtcttcca ccagcaggat ccgcttgccg ctcatgtcga
     6481 tgccggcttg tgaggactcg cggatggcgt cggatggcgg ttctgcacgc ttgtacttga
     6541 ccgtgaagaa gaagaccgag ccttctccga ctttactttt cacttcgagc tgtccgccgt
     6601 gcatctccac aagctgcttg ctgatcgtca gcccgagtcc ggtgccgccg tacttgcgcg
     6661 tcgtatccga agaagcctgt ccgaaactct cgaagatctt gtcgagccgg tcgggcggaa
     6721 tgccgatacc ggtatcgcgt acgctgaacg tgatctccgg ctcatcgtcg gttccggcag
     6781 tgaccttggc tccgatcgtc acttttccct tctcggtaaa cttgatcgcg ttacccgtca
     6841 ggttcatcag cacctgggtg atgcgcgttt cgtcgcccac cacatagaac ggcacattcg
     6901 cgtcgatgct ctcgaccagc gtgatgcctt tttcctccgc cttcaggttg aggatcgtat
     6961 ggacgttctg gatcgccgtg acgagcggga aaggaaccgc ttccagttcc atcttgccgg
     7021 cttccagctt ggcaaggtcg aggatgtcgt tgatgatgac gagcaggttc gcgccgctct
     7081 tcttgatgac gtgcaggtat ttctcctgca acggattgag cggtgaattg atcagcaggt
     7141 tcgtcaaacc gacgatcgcg ttcatcggcg tgcggatctc gtgactcatg ttcgcgagga
     7201 actggtcgcg gaacttctgc gtctcgatca ggttgtcccg cgtggtcgtg agctcctggt
     7261 tcgtgacctg cagttcctcc agcgcggcat tcaattcacg tgtacgttct ttgacctgtt
     7321 cttccagctt ctgttcgcgt gcccgcgcct cgcgcaaagc cgcgtcgttc gaactgcgca
     7381 gtcgctcgaa gttgatcgta aagatgttga agatgagctg tggatactgc tcggccaatt
     7441 cggtgaacaa ctgacgcggg atctcgatcg tctttacgtc gctcgccgcg acgatcgtgg
     7501 ccgtcgccac gccgtgtgcg agaaaactct cgcccatcac ggccatcgcc cccagttcag
     7561 ccagcagaat gtcttcgatg tagatgccga ctttcccgtc ttcaatcaca aacaatgcgg
     7621 gtgggacctg acctttatcg atcaggactc cgcccttctt atacactttt gccgaagcct
     7681 tggcaacgac ttcgtcgatc accgcctggc tgcaatgctg gaacaaggaa cacttgctcc
     7741 agtcggtctt ggtagtagat tggttcataa cgttcaaaag gcccggagcc cgcgcaaggg
     7801 gtccggccgc ttccctgtct tcgtctaaga tagaaaatta gtttttaacc agccgacctg
     7861 tcaggcttcc ggctcgcagg agataacatc cggccgaaaa ggcgctaaga tctaggcggg
     7921 ccttgccacc gaaaatgcgt tcccgctgca acagccgacc cgaaaggtcc agtaattcaa
     7981 ccgtttcggc agccgatgcc attggcacct caacatttac caaatccgcg gccggattcg
     8041 gaaaaagacg gatcccggtt atcaagtcaa attcattcgt cgccaggatc aggcaggcgt
     8101 cgtccgaaac cggcgtcgcc gtcgcttcca ggtagccagg attgctcacc gtcatccccg
     8161 aatacaggat catgctggtg tccgtcggaa cgtaggtagc ggtggtcacc accggctgga
     8221 tggcttgctc atgacaaccg gtccagccca gactgtccgt atggatcagc agcatctggc
     8281 tcaggttggc cggcgtgtaa ctcgtcgtga aaccggagat caggtacccg ccatcatcgg
     8341 ctttcgagct gcgataggct tcatcctgca aaccgtcgcc atacgccctg caccacaagg
     8401 tatcaccgtt cgcatcggtc ttgatgagca ccacattcat cgcgtcgtca ccggcagtac
     8461 gatgtccgta gcccagcgaa ttggaataac ccgacaacac aaagccgcca tcagggtccg
     8521 cgatcacggt ataagcggct tcatcaccga taccgccata cgttttcgac cacaacacgt
     8581 cgccgatgct gtcgagtcgc aacagccagc actcgtaacg ccccggaccg ttaccgaccg
     8641 atgtccccgc cgcgatgaac ccgccgtccg ccaacggcac gatggaagcc agctcttccc
     8701 acagcgtgtc accgtacatc cgcgaccaca acaagtttcc gttccggtcg gtcttcatca
     8761 ccaaggcgtt cgattgttcc aatccggccc agttgttata caccagcttg ccggcgacaa
     8821 ccagtccgct gtcggccgtc tcggcaacac acaaaccgat atcacgcgat gccgtatcga
     8881 acactttcgt aaaaatcgta tcgccattgg catcggtgcg catcaggaaa aggctgttca
     8941 acgttcccgg cacgctctgc gtcgcgccga cgaacaagag gtcaccattc ccggcctccg
     9001 ccacataggc gaatccgtca gcggctgccc ctccatacgc cttggcccaa agcaggtagc
     9061 ccagactgtc ggtcttgaac accaacgcgt cttcattccc gcccgtaata ttcaatcgac
     9121 ccgtgcacac gatccctcca tcggccgcaa cgaccgcgta ctcggaatta tcgtaacccg
     9181 cggttccata aacacgcgac cacacgat
//
LOCUS       DAOTON010000010         8975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_7053_length_8975_cov_4.336211, whole genome shotgun sequence.
ACCESSION   DAOTON010000010 DAOTON010000000
VERSION     DAOTON010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7053_length_8975_cov_4.336211"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4568
                     /locus_tag="P5292_00455"
     CDS             <1..4568
                     /locus_tag="P5292_00455"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRS37624.1"
                     /translation="SRITLRIRRPDDAALEMFVTDGVSGRALPGASVRLFTEQYDYRK
                     HVYTEHEVAVGTTGPDGSIVIPGSDETYASRITIIAGEDRLHTDGQTLYLHKQRREKA
                     TYRKDVFFTDRSLYRPGQTIYFKALMMDAGAVTSAILPNLPVTVRLIDPNNEVVDSVS
                     LRSSSFGSVHGQFRIPVGRMSGSWRLQSSEGQGFCRFQVEEYKRPTFEVKLDPTRSTA
                     RPGDRLVITGSANAYTGAAIQYGRVNYRVHRRSRLPVWYFGPRKMFPAGNAQEIAHGV
                     VATDDAGKFRFEFDAIPDAQLKPEDRPEYTYSIETDVTDISGETRSAQREVPVAYVPL
                     RVVLNLPERIAKGDAATFPVKTTNLEGQRVNTNAVVRVWQLGEPQGALRPRVWPAPDQ
                     FAMTEQEFSQRFPLDVYDNRVEPESWEQQRQVVERTFDTSLDTILDLRQLKDLPVGWY
                     RLECVAINAQRQEAKEVRHFLLFDPAGEVVPTPELAWFDASPAKTEPGSSVRILFGSR
                     LPEVHWVYEVEREGKIVTRKPIVTNGTQTSILEPVLEQDRGNFSIHLSTSVGNRVYTF
                     HHTVEVPHSDKKLDIQLETFRSELEPGKLERWKIRLRNPDQSPANAELLANLYDASLD
                     AILPHSWQTSFHGTRAQTQFVQPYYSGPIQGNAVATRWYEIPPSGWREYDQLNWFGYS
                     GWSRRYFLDAVSTDAVMIQTAVAGEAKPKGSISSEVTLGDKAEAPVPAIPNQTGSTGS
                     VEQPIRGNLQETAFFFPELKADANGDVLLEFDSPEALTRWKLMLLGHDEDLRTAYREE
                     SVVTKKNIMVMPNLPRFLRAGDRVDLPVRVDNLFPGESSLSIQLELSLLDAATRQPVD
                     DFFGNTKRIQTVTAKSGSSALGTFSLQIPERMEPVVVRIVATANGSSDGEEHLLPVLS
                     DRMLITEAKPFVVRGDSTRTVRFAAMEENRSASLKPVAFTLSYTPDPVWFAIQALPYL
                     KEYPHECAEQLFSRYFANTVSAAILKRRPGLEAIFSRWRNAEPKALLSQLERNPELKQ
                     IALEETPWLLAARDESERKRNIGLLFDLDRQRSESQTSLERLRQSQHPDGSWSWFKGM
                     PGDEYITRYIVEGMGRMSRMGVLDLTLNRDAQQMTQQALRFLDDRFLASCREQEKLAG
                     HDTLPAAIEDLHYLFVRSCFVQEAGKELQSKLDYCYKRAQIRWSRLPLLSQSMLLRVA
                     VRRGDEALRKAMVESLLERSLTNPEEGRWWKQDVPAYYWWSAPVETQANLIEALDEAK
                     AGASVVDDLKTWLLRQKQTQDWKTTRATVQACFALLNSGTDWLEHKNTAVLRLGEQML
                     DITSDAETGTGTIERSWAGDAIQSAFSRLEIRPQSGNSISWGALYWQYTERMDMVRST
                     AAPFSITKAVFVERTGNSGPVLESLADSTQLLQGDRIVIRMTLQVDRDMDYVHLADPR
                     VGGLEPEQVLSETRWQSGTCWYQSTRDAATHFFFPRLQKGTYVISYPLRAFQSGSYTC
                     GPAQLQCLYAPEFSAHSAGSRISISKE"
     gene            4779..7247
                     /locus_tag="P5292_00460"
     CDS             4779..7247
                     /locus_tag="P5292_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRS37625.1"
                     /translation="MKHTIRRKFAAAFTLLLAFAATNAWGQCLVPSGLSTTNITNTSA
                     KLNWATTTADSFLVRYNVSGSTAYLYKTVKPGTSTNTTITGLYPNLTYQWQVRTWCNN
                     GSSGAYQTTPASFTTLNQSVACVPPNLNVTSNITANGATLTWSNLITADSFMVRYNVR
                     NTTNYVWVKTPGSVRTLTLTGLLPNTSYDWAVRCICASAPSQAYSSTNTFTTLSSSCG
                     TADVAYFSSTLITHNSATVGWRSVSGALSYNVRYAVRYSGNWTTVTTTTNSRALSGLQ
                     PTTWYEFQVQTVCSSGAGNWSSSGIFQTTTATLSISRGPYLQQANTSSIFIRWRTNIA
                     SDTRVRYGTSSSSLNLSVSNSTQLTEHVIQLTGLAPNTKYFYSVGTSTTTLQGDTGNY
                     FYTHPAVGSTGAVRIWATGDFGVNTSNQRAVRDSYRNYTGSTHTNVWLWLGDNAYNDG
                     TDSEYQTNVFNQYPYQFKKWVVWPSSGNHDLHTANSALQTGPYYDNFTMPKAGEVGGV
                     ASGTEAYYSFNYANIHFVCLESYSSAYRSSTGAMATWLTNDLSANTQRWTIVYFHHPP
                     YSKGSHNSDTETELIEMRQNIMPILEAKKVDLVLSGHSHSYERSMLIRGHFGLESTFN
                     TSTMAVNSGSGIAPSVYTKTSPNYYGTVYAVVGVSGQKGSTTSGYPHNAMYTSSVTYY
                     GSAVIDVVGNQLNFKFLTSAGSIWDQFTIQKTGSVTPVASDPSQFVNDYPASTIYDIA
                     LYPNPILDDATVEFRLSKDARVRLDILDLNGRIVYSTGSEEELPAGHHRLPLNLADAD
                     LPKGIYLLRLSGEGMEDSRRFVLQ"
     gene            complement(7291..7704)
                     /locus_tag="P5292_00465"
     CDS             complement(7291..7704)
                     /locus_tag="P5292_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRS37626.1"
                     /translation="MKTHCIRLRPGQDLRHELEAFAASSQLSAAAIVTCVGSLTVAAL
                     RFASRSGTDHVPGPLEITSLVGTFSVYGSHFHLTVADADGKAFEGHLQEGSIVRTTAE
                     VVILAMEDVAFLRETDQETGYRELKIEPLDSSRTH"
     gene            7772..>8975
                     /locus_tag="P5292_00470"
     CDS             7772..>8975
                     /locus_tag="P5292_00470"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRS37627.1"
                     /translation="MEQPTHYFEDLGLCDSLLDGLDAMGFQSPTPIQAQVIPLILDGR
                     DLIACAQTGTGKTAAYLLPVLDKISRQEIPHTSALVIAPTRELALQIDQALQGFSYFT
                     HASSIPIYGGNDGMSFDREKRALTDGASIIVATPGRLLSHLNLGYVRFDQLDTLILDE
                     ADKMLDMGFLDDILRIISFLPKQRQNLMFSATMPPKIRELAKRILQDPAEVNIALSKP
                     AAGVDQKAYVVYETQKGPLLLEILKQHADKTVIVFASRKVTVKQLEKELRQAKLPVEA
                     IHSDLAQEERENVLLRFRSRTTRILVATDILSRGIDIENIGMVINYDVPGDAEDYVHR
                     VGRTARAATTGEAITLISELDQRRFGQIESLIEATVPKMPLPEKIGSGPAYDPGRRPP
                     GKGRPSGKG"
BASE COUNT         2138 a         2435 c         2401 g         2001 t
ORIGIN      
        1 taagccggat tacgttacgc atccgccgtc cggatgatgc cgcgctggaa atgttcgtga
       61 ccgacggcgt gagtggccgc gctcttccgg gtgccagcgt gcggttattt accgagcaat
      121 acgattaccg caagcatgta tatacagagc atgaagtggc agtcggaaca acgggacccg
      181 acggctccat tgtcattcct ggcagcgatg aaacgtacgc ctcccgaatc acgatcatag
      241 ccggggagga tagactccat actgacggtc agacgctgta tctccacaag caaaggcggg
      301 agaaggcgac ttatagaaag gatgttttct tcacggaccg gtcgctctac cggccgggcc
      361 agaccatcta cttcaaagca ttgatgatgg acgcgggagc ggtcacctca gcgatcttac
      421 caaatctccc cgtgacggtt cgtcttatcg acccgaacaa tgaagtagtt gattcggttt
      481 cactccgttc ttcctcgttt gggagtgtgc atgggcagtt tcgtataccg gtcggcagga
      541 tgtccggctc gtggcgactt caatcttcgg aaggccaggg tttctgcagg ttccaagttg
      601 aagaatacaa gcgcccgacg ttcgaagtga aactggatcc gacgcgttcc accgccaggc
      661 ccggagaccg gcttgttatt accggatcag ccaatgccta taccggtgca gccatccagt
      721 atggtcgtgt gaattatcgt gttcatcggc ggtcgcgctt accggtatgg tatttcgggc
      781 cgaggaagat gttccctgcc ggcaatgcgc aggagattgc acatggcgtt gtagcaaccg
      841 acgatgccgg aaagttcagg ttcgaattcg acgcgattcc cgatgcgcaa ttgaaaccgg
      901 aagatcgccc ggagtatacc tatagtattg aaacggatgt caccgacatc agtggagaaa
      961 cgcggtccgc tcagcgtgag gtacccgtcg cctatgttcc gcttcgggtg gtgttgaatc
     1021 tgcccgagcg aattgcaaaa ggagatgcgg ccaccttccc ggttaagacc accaatctgg
     1081 aaggtcaacg tgtcaatacc aatgctgttg tccgggtctg gcaattgggc gaaccacagg
     1141 gggccctgcg tccacgcgta tggccggcac ctgatcagtt tgcgatgacg gagcaggaat
     1201 tctctcagcg atttccgttg gatgtctacg acaaccgcgt cgaaccggaa tcctgggagc
     1261 aacaacggca ggtagtggaa aggacctttg acaccagcct ggacacgatc cttgatctga
     1321 gacaattgaa agatctcccg gttggatggt accgactgga atgtgtggcg atcaatgctc
     1381 agcgacaaga agccaaagag gtccggcact ttctactctt cgacccggca ggcgaagtgg
     1441 ttccgacgcc tgagcttgcc tggtttgatg caagtccggc gaagactgag ccggggtctt
     1501 ccgtacgcat cttattcggc agcagattgc cggaagtaca ttgggtatat gaagtggaac
     1561 gggaaggaaa gatcgtaacg cgaaagccca ttgtgaccaa cggaacgcaa acgtccattc
     1621 tggaaccggt cctggaacag gatcggggta atttctcgat tcatctgagt acatccgtcg
     1681 gaaaccgtgt ctatacgttt catcatacgg tggaagtgcc gcattcggat aagaagctcg
     1741 acatccagct cgagactttt cgcagcgaat tggagccggg taagctggag cgttggaaga
     1801 tccggcttcg taacccggac cagtcgccgg ctaatgcgga attgcttgcc aacttgtacg
     1861 acgcatcgct ggatgcaatc ctgcctcatt cgtggcagac gtccttccat ggcacacgtg
     1921 ctcaaacgca attcgtccaa ccttactact caggcccgat tcagggtaac gcggttgcca
     1981 ctagatggta cgaaattcca ccatctggat ggcgcgaata cgaccagttg aattggttcg
     2041 gatattccgg atggtcacgg cgctattttc tggatgcggt ttccacggat gcggtcatga
     2101 tccaaaccgc tgtcgccggt gaggcaaaac ccaagggaag catatcttct gaagtgactt
     2161 tgggagacaa agcggaggcg ccggtgcctg ccatcccgaa tcaaaccgga tcgaccggat
     2221 ccgttgaaca acctattcga ggaaatttac aggagaccgc attctttttc ccggaactta
     2281 aagcggacgc gaacggagat gtattgctcg agttcgacag tccggaagcg cttacacgat
     2341 ggaagttgat gctcctgggt cacgatgagg atctgcgtac agcttatcgc gaagagagtg
     2401 tcgtgaccaa gaagaacatc atggtcatgc ccaacctacc ccgcttctta cgtgcgggtg
     2461 accgcgttga tcttccggtt agggttgaca atctttttcc gggcgaatcc agcctcagta
     2521 tccagttgga attatccttg ctggatgcgg ctacgcggca acctgtggac gacttctttg
     2581 gcaataccaa gaggatacaa acggtcacgg cgaaaagcgg cagcagcgct ctagggacct
     2641 tttcacttca gataccagaa agaatggaac cggttgtggt gcggatcgtg gctactgcga
     2701 atggcagcag cgacggcgaa gaacacctcc tgccggtgct ttcggatcgg atgctgatca
     2761 ccgaggcgaa accatttgtg gtccggggtg attcgacccg tacggtacgg tttgcggcca
     2821 tggaggaaaa caggagcgcc tccctgaaac cggtcgcctt cactctttcg tacacccccg
     2881 acccggtctg gtttgccatc caggctttac cgtacctgaa ggagtatcct catgaatgtg
     2941 ccgaacaatt gttctcccgg tattttgcga acacggtttc cgcagctatc ctgaaaaggc
     3001 gcccgggcct ggaagcgatt ttctcacgct ggcgaaacgc cgaaccgaag gcactgcttt
     3061 cccaattgga acgaaatccg gaattgaagc agatcgcatt agaggaaaca ccctggctgc
     3121 tcgcagctcg ggatgaatcc gaacgcaagc gaaatatcgg tctgttattc gacctggacc
     3181 ggcaacgaag cgaatcgcaa acttcgctgg agcgcttgcg acagtcccag catccggatg
     3241 gaagctggtc atggttcaaa ggcatgccgg gagacgagta catcacgcgc tatattgtcg
     3301 aaggcatggg ccggatgagc aggatgggcg tactcgacct aaccctgaac cgggatgcac
     3361 agcagatgac acaacaggct ttacggtttc tggatgatcg gtttctggca agttgtcgtg
     3421 aacaggagaa actagcaggg catgatacac tgcccgccgc gattgaagat ctgcattatc
     3481 tgttcgtgcg atcctgcttt gttcaggaag cggggaaaga actgcaatcc aaactggatt
     3541 actgctataa gcgggctcag atacgttgga gccgtttgcc cctcctcagc cagtcgatgt
     3601 tgctgcgcgt tgcggtcaga cgaggtgacg aggctttacg taaggccatg gtggaatcgc
     3661 tgctggaacg atcactgacc aatcccgaag aggggcgatg gtggaagcag gatgtgccgg
     3721 cttactactg gtggtctgcg cctgttgaaa cacaagcgaa tctgatcgaa gcgctggatg
     3781 aagcgaaagc gggcgcaagc gttgtggacg atctgaagac ctggctgctt cgccaaaagc
     3841 aaacacaaga ctggaagacg acgcgagcaa ctgttcaagc ctgtttcgct ttgctgaata
     3901 gtggtaccga ttggcttgag cacaagaata ccgctgttct tcgactggga gaacaaatgc
     3961 tggatatcac ctccgatgcc gaaacaggaa ccggaacgat tgaacgaagc tgggcgggtg
     4021 acgctattca gtcagcgttt tcccgacttg aaatccgtcc gcaatcgggt aattccattt
     4081 cctggggcgc actctactgg cagtataccg aacgaatgga tatggtccgt agtacggcgg
     4141 caccctttag cattaccaaa gccgtgtttg ttgaacggac cggaaatagc ggaccggtac
     4201 tggaatcact cgccgactca acccaactat tgcaaggcga caggatcgtg atccggatga
     4261 ccttgcaggt tgaccgcgac atggactacg ttcaccttgc ggatccgcgc gtgggcggac
     4321 ttgagccgga gcaggtacta tccgaaacca gatggcaatc cggcacctgt tggtatcaga
     4381 gtacccgtga tgcagcaact cactttttct ttccgagact ccagaaaggt acttacgtga
     4441 tcagttatcc actgcgtgcg tttcaatccg gatcttatac ctgcggtccc gcacaattgc
     4501 aatgcttgta tgcgcctgag ttctcggcgc actctgcagg atcgcgtatt agcatatcaa
     4561 aagagtagtc ggctttgcac ggttcccata ggccttgaca gcttgatggg aaccgccatt
     4621 gcccgattct gacgtcgtct accaactgtt ttcatctttt cgccaaagag acgggcaata
     4681 agcctgattt tcagcgatta gaaagttttt ttgaccgtta cacttcgtta acttgtgata
     4741 cccgtacgag acgggctaat catcctgcga acatgattat gaaacacaca atcagacgaa
     4801 aatttgcagc agcatttacg ctgttgctcg catttgcagc gacgaatgca tggggtcaat
     4861 gcctggttcc atccggactc agcacgacca atatcactaa caccagcgca aagctgaatt
     4921 gggcgacaac aacggcggat agttttcttg tccgctacaa cgtgtccggt tcaaccgcat
     4981 acttatataa gacggtcaag ccgggcacca gtacgaacac gaccatcacc ggcttgtatc
     5041 cgaacctgac gtatcagtgg caggttcgta cctggtgtaa caacggttca tcaggtgctt
     5101 accaaactac tccggcatcc ttcacgacac tgaatcagtc ggtagcctgt gttccgccga
     5161 acctgaatgt cacgtccaac atcaccgcca atggcgcgac cctgacttgg agcaatctga
     5221 tcacagctga cagcttcatg gttcgctaca atgttcgcaa cacaacgaat tatgtatggg
     5281 tcaagacacc gggcagcgtg cggaccctga cacttacggg attacttccg aatacatcct
     5341 acgactgggc tgtccgttgc atctgtgcaa gcgcgccgtc gcaggcatat agttccacca
     5401 ataccttcac cacgctttct tccagctgcg gaactgctga tgtcgcctac ttttcctcaa
     5461 cactcatcac gcacaatagc gcaacggttg gttggcgatc ggtatccggc gcgttgagtt
     5521 ataatgtacg gtatgcggtt cgctactccg gaaactggac gaccgtcacg actacgacga
     5581 attcacgcgc actgagcgga ttgcagccca ccacctggta tgaattccag gtacaaacgg
     5641 tttgttcctc cggggcggga aactggtctt cctccggaat cttccaaacc acgaccgcaa
     5701 cactttccat ttcgcgcgga ccgtatctgc agcaggcgaa taccagcagc attttcattc
     5761 gctggcggac caatatcgcg agcgatacac gggtgcgtta tggaaccagc tcctccagcc
     5821 tcaacctttc ggtatccaat tcgacgcagt tgacggagca cgtcattcaa ctgacgggcc
     5881 tggcgccaaa taccaagtac ttctattctg tcggcacctc cacgacaacc cttcagggcg
     5941 ataccggcaa ctatttctat acccatccgg ccgtcggatc taccggagct gtgcggattt
     6001 gggcgaccgg cgatttcggc gtcaacacct ctaaccaacg tgcagtccgc gattcctacc
     6061 ggaactatac gggttctacg cataccaatg tatggttgtg gttgggagac aacgcctata
     6121 acgacggtac ggattcggaa tatcagacga atgtgttcaa ccaatatccc tatcagttca
     6181 agaagtgggt cgtatggcct tcgtccggta accacgatct gcatacggcg aattccgcat
     6241 tgcaaaccgg cccctattac gacaacttca ccatgcccaa agcaggcgaa gtgggaggcg
     6301 tggccagtgg tacggaagcg tattattcct tcaattacgc gaacattcac ttcgtttgtc
     6361 tggagtccta cagttcagcc tatcgttctt cgactggtgc tatggcaact tggctgacca
     6421 atgatctgag cgcgaacaca caacgttgga ccatcgtcta tttccaccat cctccatatt
     6481 ccaaagggtc acacaattcc gatactgaaa cggagttgat cgagatgcgt cagaacatca
     6541 tgccgatcct ggaagcgaaa aaagtcgatc tcgttctcag cggacacagc cactcctatg
     6601 aacgctccat gctgatccgc ggtcactttg gactcgagtc tacattcaac accagcacca
     6661 tggccgtcaa cagtggcagt ggcatagcac cgagtgttta tacgaagact tcgcctaatt
     6721 actacggtac ggtgtacgcg gtggtaggtg tctcaggcca aaagggttcc acgacttccg
     6781 gttatccgca caacgcgatg tatacctctt ccgttactta ttatggatcg gctgtcatcg
     6841 atgtggtggg caaccagctc aatttcaaat tcctgacatc ggccggttcg atctgggatc
     6901 agttcacgat ccagaaaacc ggaagcgtta cccccgttgc ttcggatcct tcccaattcg
     6961 tcaacgacta tcctgccagt accatttatg acatcgcgct ttatccgaat cctattctgg
     7021 atgatgctac ggtggaattc cgcctttcca aggacgcccg tgttcgcctg gatattttgg
     7081 atctgaatgg ccgaatcgtc tattcaacag gtagcgaaga agaattgccg gccgggcatc
     7141 atcgcctgcc gctcaatctt gccgacgcag atcttccgaa aggaatctac ctccttcgcc
     7201 tcagcgggga agggatggaa gattcccgga gattcgttct tcagtaatca aacagaaaaa
     7261 aagaaagccg gcaccaaagc cggctttttt ttagtgagtt cgactggaat ccaagggctc
     7321 gattttcaac tcccggtatc cggtttcctg atccgtttca cgaaggaatg cgacatcttc
     7381 catggcgagg atcaccactt ctgcggttgt gcgaacgatc gaaccttcct gtaagtgacc
     7441 ttcgaacgct tttccgtccg cgtccgctac tgtcaggtga aaatgggaac cgtagacgga
     7501 aaaggtgcca accagtgagg ttatctccaa tggacccgga acatgatcgg ttccggatcg
     7561 ggatgcgaat cggagcgcgg caacagtcag gctgccgacg caggtcacga tggctgctgc
     7621 ggataattgg ctggaagctg caaatgcttc caattcgtgc cgcaagtctt gtccggggcg
     7681 gagccggata caatgagttt tcatagtcga tagcttgtag aaagataagg ataccgctcg
     7741 ggataccgtt cggagggcgt acctttgctt cgtggaacaa ccgacacact atttcgaaga
     7801 tctgggccta tgcgacagcc tcctggacgg cctggacgca atgggattcc agtctccgac
     7861 acccatacaa gcccaggtga tcccgctcat cctggatgga cgggacctga tcgcctgtgc
     7921 acagaccggt accggcaaga ccgcagcgta cctgcttccg gtgctggaca agatctcccg
     7981 gcaggaaatt ccccatacct ccgcgctggt catcgcgcct acccgcgaac tggcactaca
     8041 gatcgatcaa gccctgcagg gtttttctta tttcacccac gccagttcga ttccgatcta
     8101 tggcggcaac gacggcatgt cgttcgaccg ggagaaacgc gcgctgaccg acggcgcttc
     8161 cattatcgtc gcaacaccgg gtcgactctt gagtcacctc aacctcgggt atgtccgatt
     8221 cgatcagctc gatacgttga tcctggatga agccgacaag atgctcgaca tgggattcct
     8281 cgacgacatc ctgcgcatca tcagttttct cccgaagcaa cggcagaacc tgatgttcag
     8341 cgcgacgatg ccgcctaaaa tccgggaact tgccaagcgc attctgcagg acccggccga
     8401 agtgaacatc gcactcagta aaccggcggc cggcgttgat cagaaagcct atgtggtgta
     8461 tgaaacccaa aagggaccgt tgttgctcga gatactgaaa caacatgcgg acaaaaccgt
     8521 gatcgtgttc gcgtcgcgaa aggttacggt caagcaattg gaaaaggaac ttcgtcaggc
     8581 caaattgccg gtcgaggcta tccatagcga ccttgcacag gaggaacgcg aaaacgtcct
     8641 gctccgtttt cgaagtcgta cgacccgtat tctggtggcc accgacattt tatccagggg
     8701 tatcgatatc gaaaacatcg gcatggtgat caactacgat gtgcctggtg acgccgaaga
     8761 ctatgtacac cgagtcgggc gcaccgcgcg agccgctaca acaggggaag ccattaccct
     8821 gatcagtgaa ttggatcaac gacgcttcgg gcagatcgaa agtctgattg aagcaaccgt
     8881 ccccaagatg ccgctcccgg aaaaaatcgg cagcggtccg gcttatgacc ccggtcgccg
     8941 accgcctggc aaaggacgtc catcaggaaa aggcg
//
LOCUS       DAOTON010000011         8942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_7100_length_8942_cov_3.391471, whole genome shotgun sequence.
ACCESSION   DAOTON010000011 DAOTON010000000
VERSION     DAOTON010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7100_length_8942_cov_3.391471"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="P5292_00475"
     CDS             <1..1047
                     /locus_tag="P5292_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRS37628.1"
                     /translation="PPTTYITDFEILGQSVSLKAPIVVQPDSNSIGLTYIAPTFDQPH
                     LMQFRYRFLPDDEEWTVAATTGILISDMDPGYYRFEVQAKKFNSDWGPTAFLEFRILP
                     PFYKTLAFRILMIALAVGLAFLLIRRIIARRYQKQFVYLREQQALEVERRRIGADMHD
                     DLGADLTNILIYSKIISSAEALNERQRGALKKIGETSNDLINKMNEIIWALNPSNDTL
                     DNLIAYLRQYAYDFCELHNLPLQIESSPQSQLIPIKAAVRRNLFLVLKEALRNVLRHA
                     RAHQVKVHIVNDATNRSLLLLIEDDGNGIDRSKPKHGNGLVNMQKRIAEIGGTISIDS
                     PGTKGTRIQVQVHY"
     gene            1289..2038
                     /locus_tag="P5292_00480"
     CDS             1289..2038
                     /locus_tag="P5292_00480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS37629.1"
                     /translation="MSTEQKKDLNQYYLDTYTKEASVYDQRRWTSKGGSKAKSLRNAY
                     FFDILRKYNLIQPEKKIIDVASGTGRVALELVTFGYKKVVACDLTNAMLDVSRSKLPA
                     QYKDILEYHVADMKKLPFSDASFDGATLGAFLYLIPLEEYPAYTKDIHRVLQKDGILV
                     CEVMNQLHFFNPFKAVMKFWHRHVRGKKIKSHAYPWELKKAFAPFEVVDIIGTDFPAV
                     MTIFGDGFLRFLSRSPLTKYFGGRFVVGLKK"
     gene            2042..2971
                     /locus_tag="P5292_00485"
     CDS             2042..2971
                     /locus_tag="P5292_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRS37630.1"
                     /translation="MIAQPESLYQPILEQRSLRLMEVLSEKKLRGVYKVAELSGGPPL
                     ILKVNKLFPGKLRREADFLQLAEAHELKHLKLPRIVDRSDHWMLMDCVQLEHYTRDTI
                     LLKAWTTAEAEKWIAALQEFQQIPQSSRGFSKKEKLKGAFYPLIRLIELRKAVNKRLS
                     LWRRVQLGWMALRYLAYRPLLRQVTVHYDLNTFNFTNEQGSEKMLMIDFEAGAYRGDS
                     LYDILYYLTIPTVRLEEWGFQRTLLGTWFRASEGRERFLSLRLRFQLVVLCLQRIQRF
                     IDEPEKAQVYVDNLDLLFDGNRYRNWRIRLQQA"
     gene            3028..3666
                     /locus_tag="P5292_00490"
     CDS             3028..3666
                     /locus_tag="P5292_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS37631.1"
                     /translation="MKENEPMIRVAVIEDHHDIRDGMSFLIESSDEFLCRAYGTAEEA
                     LEAFAEMPPDVVLMDINLPGMDGIACSRIIKEKYPSVHIMMCTVYEDDEKIFRALSAG
                     ASGYILKRSAGDNLLDAIRELHRGGAPMSSSIARKVVASFRQAPLSSPEAALLTPREN
                     EILDLLAQGFRNKEIAEKLHVSVNTIRTHIYNIYEKLHVQNRIEALNKTGRI"
     gene            complement(4109..7552)
                     /locus_tag="P5292_00495"
     CDS             complement(4109..7552)
                     /locus_tag="P5292_00495"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRS37632.1"
                     /translation="MKKLLLLLAAMGAALLTSAQCTTSNATSCVCPAGASNCDLLPDI
                     SVGKDAITASGGYTEYAQINAGTSQSGQGSDDGRLRLTGSTPNIGYGPMEVRGVSKWV
                     CGTDTVTAYPGTGTCPDGSDPKQVVAQRVYHKNGSVMTYSDREAGTMTYHPTHGHQHI
                     DGWGFYTLRIENPTDPNPLNWPIVGSGSKLAFCLLDLSNCNSSNGHCRDSLNNILTST
                     NLPNYGLGGGQYGCSNTLQGISAGYVDIYSKTLDGMWINIPQGTCNGNYWIVVQIDPN
                     NNFQELRENNNVIAVPITLTRQTAPGTGVATISSNRAGVIAPGDVATLTANAGTAYTW
                     STGATTQSIQVSTAGTYSVTVTSPCGTATSAPYTLSVLTAPTAPSVTNASRCGSGSVT
                     LGASGSGTLKWYTGASGGISIATGSSYTTPTLSATTNYYVSNSVTSSGTNSSAGPASI
                     TIGAGAYNTSSTAQYLVFNVYSNIVLQSIKVYSNTAASRTFTLFDKNLSTLRTTTVPL
                     VNGTNTVTLNWSIPPGDFYRIQSSGTGMYYNTAGVSYPYNYPGLLQIHNSSGGHGQYY
                     YFYDWQVRRADIVQESARTMVTATINPIPSVSFTGLAASYTTGSSPAMLTGSPSGGTF
                     SGPGISGNTFTPSVAGAGGPYTVTYTYTNAFGCTGTATATTTVTAAGNNCLRPTGLGS
                     SAVTNVSATVSWSNAATADTFRIRYSVNGTTNYLYKDVNGAGGVSSTGLSGLSPGTTY
                     QFQVSSICRGVSSGYSNSATFTTTNAPIPCAVPNALSTTNIANNSATVGWTPFVTADV
                     FRIRYYVNGTSTYFYKDLNGSSGSSTSLTNLTQNTTYKWQVSSICNGVSSGYSSVATF
                     TTTNNPTPCGRPFGLGTSNVANTSATVSWTNLVTADIFRIRYSVNGTTNYLYKDVSGV
                     GGTTSASLTGLTPNTTYQFQVSSICSGLSSGYSTALTFTTTNAPVPCATPYGLSSTNV
                     TNNSAVVNWTPMVTADSFMVRYSVNGTTNYLWKKVSGAGGVTSMSLTGLSANTTYQWQ
                     VRSICNGVTTSIYSSSALFTTGLIRQGNFGTTDESDALTAFPNPAHENVQLRYFSGLF
                     GKARLTVYDLSGRNLLMETKTVMEGENRFELSLKDLQSGTYLIRLDQGEQTKVIRIVK
                     E"
     gene            complement(7731..>8942)
                     /locus_tag="P5292_00500"
     CDS             complement(7731..>8942)
                     /locus_tag="P5292_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37633.1"
                     /translation="TLGGLWTNIFLASIDPNGQVNWKRNLTRENPDAYEVGCLARHPD
                     GSVWYAHSDFSTSVICRLDVNGNEQRVDSVSGIRILSGLAFDPFGNRYLSGANESGTL
                     TVNNLSISAITSYGQFVARVRADGTAHWAYSGNDITFQRPEVVAGDDGSVSLSCAVFD
                     SITWGNISLPSPGNAQTFYLVHADSTGFFTWGRQMPVGADPFSYLQPLAQHYLSRLSD
                     GTIVLIGNQRATVDWGNGVVLSSGSSFSQSTAILYFHPADGSVIHGLIGGSTGYDFTQ
                     SLAVGPANEVWFTSTVTDSATYGPFLVNTGEAYRTIVGKVSAPLPSGVSGIAASDAVV
                     FPNPTSDLLWVRGQAPGTSARILSLDGRLLESRQADAGTVFSLQQYPAGVYLLQLINE
                     TGVRCLKVIRN"
BASE COUNT         2045 a         2367 c         2524 g         2006 t
ORIGIN      
        1 ccgccgacga cctacatcac cgattttgaa atcctcgggc aatcggtatc gttgaaggcg
       61 ccgatcgttg ttcagcccga cagcaattcg atcggactga cctatatcgc accaactttt
      121 gatcaacccc atctgatgca gttccgttac aggtttctgc ccgatgatga ggaatggacg
      181 gtcgcggcta cgacgggtat cctgatcagt gatatggatc ccggctacta tcgcttcgag
      241 gtacaggcga agaaattcaa ttccgactgg ggcccaacgg cgtttctgga attccggata
      301 ctgcccccct tttacaaaac actggcattc cggatcctga tgatcgccct cgcggtcggg
      361 ctggcgttcc tgctcattcg tagaatcatc gcccgccggt accaaaagca gttcgtttat
      421 ttacgcgaac agcaggcgtt ggaagtggag cgtcgaagaa tcggcgcgga tatgcacgac
      481 gatctgggcg ccgatctcac caacatcctg atctattcca agatcatcag ctccgccgaa
      541 gcattgaacg aacggcaacg gggcgcgctg aaaaagatcg gtgaaacctc gaatgatctc
      601 atcaacaaga tgaacgagat catctgggcg ctcaaccctt cgaacgacac cctcgacaac
      661 ctcatcgctt atttgcggca gtatgcctac gatttttgcg aattgcacaa cctgcccttg
      721 caaatcgaat cctctccgca atcgcaactc ataccgatca aagccgccgt tcgccggaac
      781 ctgttcctgg ttttgaaaga agcattgcgg aacgtgttgc gccatgcccg ggcacaccag
      841 gtgaaagtgc atattgtaaa tgatgcgacc aaccggagcc tgctcttgct gatagaagac
      901 gacggcaacg gcattgaccg gagcaagccc aaacacggca atggtctggt caatatgcaa
      961 aagcgtatcg cggagatcgg tggaacgatc agcattgatt cgcccggtac caaaggcacg
     1021 cgtattcaag tgcaagttca ctactaagca acccgaagaa agaccgccgc agacgcgagc
     1081 agcggttttt gcccaatcat ccccctgcga gacgtatctt ttttcgatga tttacgtctt
     1141 aatgcagtgg gagagcatgc tgagtttcaa tctgcagcca gcttggaccc ttctcacaat
     1201 aacactaata aactaatatt caataagata caggcggctt catcctgcgt acgttgtaga
     1261 ctccccgcga tcttatccca cccgttacat gagcactgaa caaaagaagg acctgaacca
     1321 gtattacctc gatacttata cgaaggaagc gagtgtttat gatcagcgtc gctggaccag
     1381 taaaggcgga agcaaggcga agagtctgcg taacgcgtat ttcttcgaca tccttcggaa
     1441 gtataacctg atccaaccgg agaagaaaat catcgatgtg gcttccggta ccggccgcgt
     1501 tgcgttggag ttggtgacct tcggctataa gaaagttgtc gcctgtgatc tcaccaacgc
     1561 catgctggat gtcagtcgct cgaaattgcc ggcgcagtac aaggacatcc tcgagtatca
     1621 tgtcgccgac atgaaaaagt tgccgttttc ggacgcatca ttcgacggcg ctacactggg
     1681 tgcatttctc tacctgatcc cgctcgaaga atatccggcg tataccaagg acatccatcg
     1741 cgttctacaa aaggacggca tcctggtttg cgaagtgatg aatcaattgc acttcttcaa
     1801 tccgttcaaa gcggtcatga aattctggca ccgccatgtc cgcggtaaga agatcaagag
     1861 tcatgcgtat ccatgggagt tgaaaaaggc gttcgcgcca ttcgaagtgg tcgatatcat
     1921 cggcaccgac ttccccgcgg tcatgacgat tttcggtgac ggttttctcc gctttctgtc
     1981 gcgttcaccg ctgaccaagt atttcggtgg acgttttgtc gtcggactaa aaaagtaagc
     2041 gttgatagcg caaccggaat ccctttacca gccgatcctg gagcagcggt cgctccggct
     2101 gatggaggtg ctttcggaaa aaaagctgcg cggcgtctat aaagttgcgg agctgtcggg
     2161 cggccctccc ctgattttga aagtgaacaa attgtttcct ggcaagttgc gccgggaagc
     2221 ggactttttg caattggccg aagcgcatga attgaagcac ctgaaacttc cgcggatcgt
     2281 ggaccggagc gatcactgga tgttgatgga ctgtgtgcaa ctcgaacact atacccgcga
     2341 taccatacta ctgaaagcct ggacgacggc ggaagcggaa aaatggatcg cggccctgca
     2401 ggagtttcaa cagatcccgc agtcgtcgcg tggctttagt aaaaaggaaa agctgaaagg
     2461 ggccttctat ccgctgatcc gtctgatcga gctccgaaaa gccgtgaaca agcggctgtc
     2521 actgtggagg cgcgtgcaac tggggtggat ggccctgcgc tacttggctt atcgtcctct
     2581 gctccgccag gtgaccgtgc actatgatct gaacaccttc aatttcacca atgaacaggg
     2641 ttcggaaaag atgctgatga tcgatttcga agccggcgcc taccggggcg attcgcttta
     2701 cgatatttta tattatctta cgatacccac ggtacgactg gaggaatggg gatttcagcg
     2761 aacgctgctc gggacctggt ttcgagccag cgaaggcagg gaacgctttc tgtcgctgcg
     2821 gctccggttt caattggtcg tcctgtgttt gcaacgcatc cagcggttca tcgatgaacc
     2881 ggagaaggca caggtctatg tagacaacct cgacctgctc tttgacggca atcgataccg
     2941 gaattggcgg attcgtttgc agcaagcttg agccggcttg cgtaacttgc gaccggttat
     3001 atgcaactac ttttagactg aagcaacatg aaggagaacg agccgatgat ccgcgtagcg
     3061 gtgattgaag atcaccacga catccgtgac gggatgtcat tcctgatcga gagcagtgac
     3121 gaattccttt gccgtgctta cggcactgcg gaagaagcgc tggaagcctt tgccgaaatg
     3181 cctcccgacg tcgtcttgat ggatatcaac cttcccggca tggatggtat cgcgtgttcc
     3241 aggatcatca aggaaaagta cccgtcggtg cacatcatga tgtgtaccgt gtacgaagac
     3301 gacgagaaga tcttccgtgc gctttccgcc ggtgcgagtg gatatattct gaaacgttcc
     3361 gccggagaca atttgcttga cgcgatccgc gaattgcatc ggggcggagc gcccatgagc
     3421 agctccatcg cgcgtaaagt ggtggcttct ttccgccagg cgccgctctc cagcccggaa
     3481 gccgctttgt taacgccgcg tgaaaacgaa atcctcgatc tgcttgcaca gggattccga
     3541 aataaagaga tcgccgaaaa actacacgtc agcgtaaaca cgatccgtac acacatctat
     3601 aacatctacg aaaagctgca cgtgcagaac aggatcgaag cgttgaataa gaccggacgg
     3661 atctagcagt agcgacgaag ggcaaagggt tgtttgttcc gggttccggg ttgagtggta
     3721 gggacgtggg acgagggacg aactgcgaga aagataacta gcctgaattt tcaaccttca
     3781 accttcaacc ttcaactgtc aaccctagtt aggcattgac aattgacaac gcggatcccg
     3841 gaacccgaaa cccggaacat tgcagcccaa aatcatccat ttgtcgtatt ggccttgggt
     3901 ccttgccttg cgacctttgc accactgagt acagccttcg ggcaccccct tcttctcata
     3961 gtagttattt atcagttttt catgctcatc tgaagatccc gcgcgcaggc gggatcttct
     4021 ctttcagagc aggttgtgtt ttggcaataa aaaaccccct tgttgattca acttgggggt
     4081 tttttacgtc aaagacgttt taaaataatc attctttcac gatccggatc accttggttt
     4141 gttcaccctg atccaggcgg atcaggtagg tgcccgactg aagatccttt agggaaagtt
     4201 cgaagcgatt ttctccttcc atgaccgttt tcgtttccat cagcaggttg cgaccactga
     4261 ggtcataaac ggtcaggcgg gcttttccga agagtccgga gaaatagcgc aactgaacat
     4321 tttcgtgtgc aggattcggg aaggccgtca gcgcatccga ctcatcggtc gttccgaagt
     4381 tgccctgacg gatcagacct gtggtgaaca atgccgagga actgtaaatc gaagttgtaa
     4441 caccgttaca gatcgagcgc acctgccact gataggtcgt gttcgcggag agaccggtaa
     4501 gcgacatgct ggtcacgcca ccggctccgc tgactttttt ccagaggtag ttcgtcgtac
     4561 cgtttaccga gtagcgtacc atgaaactgt cggccgttac catcggtgtc cagttgacaa
     4621 ccgcgctgtt gttggtcaca ttcgttgaac tgagtccgta cggagtcgcg cacggcaccg
     4681 gtgcattcgt cgtggtaaag gttaacgcgg tactgtaacc gctgctaaga ccggaacaga
     4741 tcgaagatac ctggaactga taggtcgtat tgggcgtcag accggtcagg ctcgcagaag
     4801 tcgttccgcc tacaccgctg acatctttat agagataatt cgtagtgccg ttgactgaat
     4861 agcgaatacg gaatatgtcg gcggtcacca ggttggtcca gctcacggta gcgctggtgt
     4921 tcgccacgtt cgaagttccg agaccgaagg gtcggccgca cggagtcgga ttattcgtcg
     4981 tcgtaaacgt agcaacggag ctataaccgc tgctcacacc attgcagatc gaactgacct
     5041 gccatttgta ggttgtgttt tgtgtcaggt tcgtcagcga tgtgctgctg ccggaacttc
     5101 cgttgaggtc tttgtagaag tacgtagacg ttccgttcac ataataccgg atacggaata
     5161 catcggcggt aacgaaaggt gtccaaccta ccgttgcgct gttgttcgcg atgttggtcg
     5221 tgctaagcgc gttggggacc gcgcagggaa tcggcgcgtt ggtcgtggta aaggtggcgg
     5281 agttgctata accgctgctc acgccgcggc agatggaact gacctggaac tggtaagtcg
     5341 ttcccggaga gagtccgcta aggccggtgc tgctgacacc acctgcgcca ttgacatcct
     5401 tatacagata gttcgtcgtg ccgttcaccg aataccggat ccggaacgtg tcggccgtgg
     5461 ccgcgttgct ccagctcacc gtcgcggaaa cgttggtgac cgcggaggag cccagtccgg
     5521 taggacgtag acaattgttc cccgcagcgg taaccgtggt agtggccgta gccgtgcccg
     5581 tgcaaccgaa tgcgttcgtg taggtgtagg ttacggtata cggaccacct gcgccagcaa
     5641 ctgaaggcgt gaacgtattg ccgctgatgc cgggacccga aaacgttccg ccggaaggag
     5701 agccggtcag cattgccggt gaagaaccag tcgtgtacga tgcggcaaga ccggtgaatg
     5761 aaaccgacgg aatcggattg atcgtagccg tcaccatagt gcgcgcactt tcctggacga
     5821 tatcggcgcg ccgcacctgc cagtcgtaga agtaataata ctgaccatgt ccacccgatg
     5881 agttatggat ctgcagcaat cccggatagt tgtacgggta agaaacaccc gccgtgttgt
     5941 aatacatgcc ggtgccactg ctctggatgc ggtaaaaatc gccgggcggg atgctccagt
     6001 tcagggtgac cgtattcgta ccgttcacca gcgggaccgt cgtggtgcgc agggtcgaaa
     6061 gattcttatc gaacaaggtg aaagtacgac tcgcggctgt gttggaatac accttgatcg
     6121 actgcaggac gatgttggaa tacacgttga ataccaggta ttgcgccgtg ctgctcgtgt
     6181 tgtaggcgcc ggcgccgatg gtaatgctgg cgggaccggc actgctgtta gtgccggagc
     6241 tggtcacgct gttcgataca taatagttcg tggtcgcgct gagcgtgggg gtggtgtaac
     6301 tcgatccggt agcaatggat attccacccg atgcgccggt gtaccacttc agggtcccgg
     6361 atccgctggc ccccaaggta accgaaccac ttccacatcg actcgcattc gtcacggacg
     6421 gggcagttgg ggcggtcagc acactcaagg tgtagggagc ggaggtcgcg gtgccgcaag
     6481 ggctggtgac ggtcacacta taggtgccag cagtgctgac ctggatcgac tgtgtcgttg
     6541 cacccgtgga ccaggtgtag gccgttccgg catttgccgt aagggttgcc acatcgccgg
     6601 gagcgatcac acctgcgcga ttgctgctga tggtcgcgac accggtaccc ggcgccgtct
     6661 gccgggtgag ggtgatcggt accgcgatca cgttgttgtt ctcacggagt tcctgaaaat
     6721 tgttgttggg gtcgatctgc accacgatcc agtagttgcc gttacaggtt ccctgcggaa
     6781 tgttgatcca catgccgtcc agcgtcttcg aataaatatc gacgtagccg gcggagatac
     6841 cctgaagcgt attcgaacaa ccatactggc cgccaccgag tccgtagttg ggcaggttgg
     6901 tgctggtgag gatattattg agggagtcgc gacaatgacc gttgctgctg ttgcaattgc
     6961 tcaggtcgag caggcagaaa gcgagtttgg aaccgctgcc gacaatcggc cagttgagcg
     7021 gattcggatc ggtagggttc tcgatgcgca gcgtgtagaa tccccagcca tcgatgtgct
     7081 gatggccgtg tgtagggtgg taggtcatcg tgcccgcttc acgatcggaa tacgtcatca
     7141 cgctgccgtt cttgtgatag acgcgctgcg cgacaacctg cttgggatcc gatccatcgg
     7201 gacaggtgcc ggtgccggga taagcggtca ccgtatcggt gccgcaaacc cacttcgaaa
     7261 caccgcgaac ttccatcgga ccgtacccga tgttcggtgt agagccggta agtcgcagac
     7321 gtccgtcatc ggaaccctgt ccgctttgac tggtaccggc gttgatctgg gcatattcgg
     7381 tatagccgcc ggaagcggtg atcgcatcct tgccaaccga aatatcgggt aacaggtcgc
     7441 agttggacgc gccggccgga cacacacaag aggtcgcgtt cgaggtcgtg cactgggcac
     7501 tcgtcagcaa agccgcaccc atggcggcca gcaacagtaa cagttttttc atgtgggatc
     7561 gcagcagttg atacgtcaag atagtgcgca tttcgatatt ttcaaaacac tcctgaaatc
     7621 aacgaaaaag cagccccaat gaatggccgg aaggcaggtt accgtttcat cagctacgct
     7681 cgacggctcc ctttttctct tgcggcagac ttgtagatga gtacggcgac tcagttccga
     7741 ataaccttca gacagcgcac accagtctca ttgatcagtt ggagcagata aactccggcc
     7801 gggtactgtt ggagtgagaa caccgtgccc gcgtccgctt gccgactctc cagcaatcgt
     7861 ccgtccaaag aaagtatccg ggcacttgtt ccgggcgctt gtccgcgaac ccacaacaga
     7921 tcactggtcg ggttcgggaa tacgaccgca tcggaagccg cgataccgct gacgccgctg
     7981 ggcaatggcg cactcacctt gccaacaatg gtacggtagg cttcgccggt attgacgagg
     8041 aacggtccgt aggtagcgct gtcggtaacg gtggaggtaa accagacttc attggctggt
     8101 ccgacggcga gcgattgggt aaaatcgtat ccggtcgatc cgccgatgag gccgtggatc
     8161 acactgccat cggcagggtg gaaatagagg atggcggtgc tttgtgaaaa cgagctgccg
     8221 gatgacagca cgacaccatt accccaatcc acggtcgcgc gttgattccc gatcaataca
     8281 atggtgccgt cgctcagcct gctgaggtaa tgttgcgcaa gcggttgcaa gtaggagaac
     8341 ggatctgcgc cgaccggcat ctgacgtccc caggtaaaga agccggttga gtcggcgtgt
     8401 accaggtaaa acgtttgcgc attccctgga gacggtaagg agatattccc ccaggtgatg
     8461 gaatcgaaca cggcacagga taggctgacc gatccatcgt cgcccgctac gacttcgggt
     8521 cgttgaaacg taatgtcgtt gccggagtag gcccaatgcg cggtaccgtc cgcgcgcacg
     8581 cgtgctacga actgtccgta ggaggtgatc gcgctgatgg aaaggttgtt cacggtcagc
     8641 gtccccgatt cattcgcgcc ggagaggtaa cgattgccga aaggatcgaa cgcgagacca
     8701 ctcaggatgc ggatgccgga aacgctatcg acccgttgct cgtttccgtt aacatcaagt
     8761 cggcagatga cactggtgga aaaatcactg tgtgcatacc aaacacttcc gtcaggatga
     8821 cgcgccaggc aaccgacctc gtatgcatcc ggattttcgc gtgtcaggtt gcgtttccaa
     8881 ttcacctgcc cgttcgggtc gatggaagcc aggaagatgt tcgtccaaag accccccagc
     8941 gt
//
LOCUS       DAOTON010000012         8924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_7122_length_8924_cov_4.440185, whole genome shotgun sequence.
ACCESSION   DAOTON010000012 DAOTON010000000
VERSION     DAOTON010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8924
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7122_length_8924_cov_4.440185"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="P5292_00505"
     CDS             <1..1086
                     /locus_tag="P5292_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRS37634.1"
                     /translation="VATGATVVASQALISASFTLINEAIRLNFWPRVRIKYPTDLKGQ
                     IYIPSINWMLYAGCVGVVLFFRESSHMEAAYGLTINIDMIMTSTLLVFYLSLRRYRSF
                     IIFLFIAVYATIEFSFLIANLDKFIHGGYVSMIISLAMIYVMWAWYQARKIRNRFVEF
                     VKLKDYLPLLRDLSHDFSIPKYATHLVYLTSANYTEEIEAKVVYSILQKQPKRADIYW
                     FVHVDVMDEPYMMEYKVVELIKGNIVRVDFRLGFRVEPRINLMFRKVVEDMVKNFEVD
                     ITSRYESLGRKNLIGDFRFVVMEKFLSLENDLPFYEKIILDTYFFFKRVSLSEERAFG
                     LDTSSVTIEKVPMVVNPVGDIPLKRVK"
     gene            1094..1609
                     /locus_tag="P5292_00510"
     CDS             1094..1609
                     /locus_tag="P5292_00510"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRS37635.1"
                     /translation="MSLKAKKASESFSILTEFVLPNDTNTLGNLMGGRLLHWMDIAAA
                     IAAHRHCGRVVVTASVNNVSFNQPIRLGEIVTLESKVSRAFNSSMEVFIDVWVENNAT
                     GEKKKCNEAIYTFVAVDQLGNPIHVPELIPETDAEKRRYEGALRRRQLSLILAGKMKP
                     ADATELKALFL"
     gene            1802..3739
                     /locus_tag="P5292_00515"
     CDS             1802..3739
                     /locus_tag="P5292_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRS37636.1"
                     /translation="MNKQAPHAVSFATLLVTLGIIYGDIGTSPLYVFKAIIGKNPIDP
                     DLVLGGISCVFWTLTIQTTFKYVILTLRADNKGEGGIFSLYALVRRTKTKWLLLPAII
                     GGSALLADGIITPPISVASAIEGLRVYNDSIPTVPIVIGIITALFLIQQFGTKFVGRF
                     FGPVMMIWFLMLGTLGVAEITQFPSVFKALNPYYAIHLLTQYHHGFWVLGAVFLCTTG
                     AEALYSDLGHCGRQNIRITWGFVKTMLVLNYLGQGAWLMQHQGQMLEDVNPFYAIMPS
                     WWLVPGIVIATAAAIVASQALISGSFTLINEAIRLNFWPKVRIVYPSDLRGQLYVPSV
                     NWLLLAGCIGIVLHFRESSGMEAAYGLAIIITMLSTTLLLMYYLYLRRVSYAVIGLLL
                     VLFLTVELSFLVANLEKFPHGGWLTLIIASMLIGVMWSWYAARKIRNRFVEFIKLKDY
                     LPMLRDLSHDFSIPKYSTHLVYLTSANFPEEIESKVVYSILQRQPKRADIYWFVHVDV
                     LDEPFTMDYKVTELIKGNIFRVDFRLGFRVEPRINLMFRNVVEDMVKNFEVDITSRYE
                     SLSRKNMIGDFRFVVMEKFLSYENELSAYEKIIMDIYFFFKRISLSEPRAFGLDTSSV
                     TVEKVPMIVNPIGRLSLRRIT"
     gene            complement(3930..5369)
                     /locus_tag="P5292_00520"
     CDS             complement(3930..5369)
                     /locus_tag="P5292_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37637.1"
                     /translation="MKYWLAWFILLFHVSATAQLTWTTSGDVAPDTCSNMHPRLCLDG
                     AGNPLVVWGRMNDQTVFFSRWTGSAFSAPVILNPPWMPVATASWMGPDIASHGDTVYV
                     VVKRIPESADSNRIFLFASFNGGVSFSNPVELGFIADSISRFPAVTTDATGNPIVAFM
                     KFNSSFLDSRWAVLRSSDYGNTFTTDTKASGWGGSAEVCDCCPGEIISEGNTCVMLYR
                     DNNANLRDSWAGISADNAISFTNGCAVDDNNWVIAACPASGPDGVIVGDTLYATFMSG
                     GTGSYQAYLSKTCLGSNTLCSIEPLTGNIPGLSQQNYPRIANDGSAIAIVWKQSVNGD
                     AQLPILLTPDITNGFPASYDTVAVNNVTNADVKLKNSRVWVVWQDNASGTVRYKTGTY
                     SAPTGLREEFDTNPFSVFPNPAGEELNVASDLSSVFTIRVYTVLGKLVYQETVQGKTT
                     VSLTDWSEGLYLMALEAGDRVYRKKFFKH"
     gene            complement(5710..6705)
                     /locus_tag="P5292_00525"
     CDS             complement(5710..6705)
                     /locus_tag="P5292_00525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRS37638.1"
                     /translation="MSKFLLLRFSSIGDIVLTSPVIRCLKEQLPGAEVHFLTKRSFEP
                     VLQHNPYIDRLHLTGTGDQLPLRELKAERFDAIIDLHHNLRTLRVAAALGVKRYAFRK
                     LNVEKWLRVNLKVDRLPKLHIVDRYLETVAGFGVRNDGKGLDYFISADEEQEGLSILP
                     PSHRNGYIGFVIGARHETKRMPDRNLVTLCGMLKRPVVLLGGKEDAELGEFIRHRVGE
                     TVFNACGKSGLNASAALVKHADSIITHDTGLMHIAAAFGKRIVSVWGNTIPEFGMYPY
                     KKQGSTEPIFIEEVKNLSCRPCSKIGYGKCPKGHFRCMRDQNLFAIAEHTLGRDT"
     gene            6794..7555
                     /locus_tag="P5292_00530"
     CDS             6794..7555
                     /locus_tag="P5292_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017242690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRS37639.1"
                     /translation="MVDTHTHLYADAFNADRPDLIKAAMAKGIGRFYLPNIDAASIGP
                     MLELEAAYPGVCIPMMGLHPCHVQQNWQAALKRVEAELVHRKWAAIGEIGIDLYWDKT
                     YFAEQQQAFLQQVAWANERQLPVVIHSRESFAEIIELLDRQPKASPQGIFHCFTGTIE
                     QARQAIDRGFYLGIGGVVTFKKSGLDAVVEQIPLEHLVLETDAPYLAPTPHRGKRNLP
                     EYVYLVAEKIAEIKGVTIEEVAKVTTENANRIFGI"
     gene            7801..8835
                     /locus_tag="P5292_00535"
     CDS             7801..8835
                     /locus_tag="P5292_00535"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I asparaginase"
                     /protein_id="HRS37640.1"
                     /translation="MSRKTTSSRKKILLIYTGGTIGMMKDHHTGALRPVDFSAIVEQV
                     PELRQLNCSFGFHAFGLPIDSSNVQPEFWGTLARVIEREYARYDGFVILHGTDTMAYT
                     ASALSYMLQNLDKPVLLTGSQLPLGAIRTDARRNLITSVEIIAGDVLVPEVCICFSNQ
                     LYRGNRAEKYTSSKFDAFHSLNYPVLAEAGTHIVFDKPEIRKKPTGKFRVKYGFNTEV
                     ALLKIYPGIHTEAMRAILSTPGLRAAIIETYGSGNAPTDPGFLRVLKEAIDRGITLIN
                     ISQCSGGSVEQGKYETSLHLQEIGVVSGMDMTTEAAITKAMFLLGQGYKGDRFERAMV
                     KSLAGEVGVF"
BASE COUNT         2062 a         2412 c         2392 g         2058 t
ORIGIN      
        1 gtggccaccg gggcaacggt cgtggccagt caggcgttga tcagcgcatc ctttacgctc
       61 atcaatgaag cgattcgcct caacttctgg ccgcgcgtac gcatcaagta cccgacggac
      121 ctgaaagggc agatctatat tccttcgatc aactggatgt tgtatgccgg ttgtgtgggg
      181 gtcgtcttat tcttccgcga atcctcgcac atggaggccg catatggtct gacgatcaac
      241 atcgacatga tcatgacctc gacgctgttg gtgttttacc tctccctgcg tcgttatcgt
      301 tcgttcatca ttttcctgtt catcgcggtc tatgccacga tcgaattctc tttcctcatc
      361 gccaacctcg acaagtttat tcacggcggc tatgtgagca tgatcatcag cctggccatg
      421 atctatgtga tgtgggcctg gtaccaggcg cggaagatcc ggaaccgctt tgttgaattc
      481 gtcaagctca aagattacct gccgctgttg cgcgacctca gccacgattt ctctatcccc
      541 aaatatgcca cgcacctggt ctaccttacc agcgcgaact acacggagga gatcgaggcg
      601 aaagtggtgt attcgatctt gcagaaacag cccaagcgcg ccgacatcta ctggttcgta
      661 cacgtggatg ttatggatga gccgtacatg atggaataca aggtggtgga gttgatcaaa
      721 ggcaacattg tccgcgtgga cttccgcctg ggcttccgcg tggaacccag gatcaacctg
      781 atgttccgga aggtggtcga agacatggtg aagaacttcg aagtcgacat tacctcccgt
      841 tacgaatcat tgggacggaa aaacctgatc ggcgatttcc gcttcgtagt aatggaaaag
      901 ttcctgagcc tcgagaacga cctgccgttc tacgaaaaga tcatcctcga cacctacttc
      961 ttcttcaaac gcgtctcgct ctccgaagaa cgggcattcg gactcgatac cagttctgtc
     1021 accatcgaaa aagttccgat ggttgtgaat ccggtcggag acatcccgct caaacgggtt
     1081 aaataacatc ctcatgtcac tcaaagctaa aaaggccagc gaatcgtttt cgatcctgac
     1141 cgaattcgta ttgcccaacg acaccaatac gctgggcaac ctcatgggag gccgcctgct
     1201 gcattggatg gacatcgccg ccgccatcgc cgcgcatcga cattgcggcc gcgtagtcgt
     1261 aaccgcctcg gtcaacaacg tatccttcaa ccagccgatc cgacttggcg agatcgtgac
     1321 cctggagtca aaggtgtcgc gcgccttcaa ttcttccatg gaagtcttca tcgacgtatg
     1381 ggtggagaac aacgcgaccg gagagaagaa gaagtgcaac gaggccatct ataccttcgt
     1441 ggccgtcgac cagcttggca acccgatcca tgtacccgaa ttgattcccg aaacggatgc
     1501 agaaaaacgc cgctacgaag gagcccttcg tcgccgacaa ctctccctca tcctcgccgg
     1561 taaaatgaaa ccagccgacg ccacggagct gaaggcgctc tttctctgag agagttgttt
     1621 gcttcgagtt tcgggtttca ggttgtcaat gcctgaccag ggttgcaggt tgcaggttgc
     1681 aggttgaaaa agaaatttca tttagaacca tagcgtacat caaaagggga aaccccaaga
     1741 ccagaagtta aacgcgaaac ccggaacccg aaacccggaa caactaaccc tcatcccctt
     1801 cgtgaacaaa caagcccctc acgccgtttc cttcgccacc ctgttggtga ccttggggat
     1861 catctacggc gatatcggaa cctcgccgct gtatgtattc aaggcgatca tcggtaagaa
     1921 tccgatcgac cccgatctgg tgctgggtgg gatctcctgc gtcttctgga cgctgacgat
     1981 ccagacgacg ttcaagtatg tgatcctgac cctgcgggcc gataacaaag gggagggggg
     2041 catcttttcg ttgtatgccc tggtgcggag gacgaagacc aagtggttgc tgcttcccgc
     2101 gatcattggc ggatccgccc tgttggccga cgggatcatc acgccgccga tctcggtcgc
     2161 atccgcgatc gagggtctgc gggtttataa cgacagcatc cctacggtgc cgatcgtaat
     2221 cggcatcatt acggcgctct ttctcattca acagttcgga acaaagttcg tcgggcgttt
     2281 cttcggtccg gtgatgatga tctggttcct catgctgggt acgttgggcg ttgccgagat
     2341 cacccagttt ccttcggtat tcaaagcact gaatccgtac tacgcgattc acctgctcac
     2401 gcaatatcac cacggcttct gggtgttggg ggcggtcttt ctctgcacaa cgggagcgga
     2461 ggctttgtac agtgatctcg gtcactgtgg tcgacagaac atccgcatca cctggggttt
     2521 tgtaaagacc atgctggtgc tgaattacct cggacagggc gcctggctca tgcaacatca
     2581 ggggcagatg ctggaagacg tcaatccgtt ctatgccatc atgccttcct ggtggctggt
     2641 gccggggatc gtcatcgcca ccgccgcggc gatcgtggca tcccaggcgc tgatcagcgg
     2701 atcgttcacg ctcatcaacg aagcaatccg gctgaacttc tggccgaagg tgcggatcgt
     2761 atatccgtcg gatcttcgcg gacaactata cgttccatcg gtgaactggc ttttacttgc
     2821 gggctgtatc gggatcgtat tgcacttccg ggagtcgtcg ggcatggaag ccgcctacgg
     2881 actcgccatc atcatcacca tgcttagcac cacgctgctg ctcatgtact acctgtacct
     2941 gcgcagggtc tcgtacgcgg tgatcggctt gttgttggtg ttgttcctca cggtggaact
     3001 atccttcctc gtggcgaacc tcgaaaagtt cccgcacggc ggctggctga cgctgatcat
     3061 cgcgtcgatg ctgatcgggg tcatgtggtc gtggtatgcc gcgcggaaga tccgcaaccg
     3121 cttcgtggag ttcatcaagc tcaaggacta cctgccgatg ctgcgcgacc tgagccatga
     3181 tttttcgatt ccgaaatatt ccacgcacct ggtctatctg accagcgcca acttcccgga
     3241 ggagatcgaa tccaaggtcg tgtactccat tcttcagcga cagccgaaac gcgccgatat
     3301 ctactggttc gtgcacgtcg atgtgttgga cgagccgttc acgatggatt ataaagtcac
     3361 ggagctgatc aagggcaaca tcttccgcgt cgacttccgc ctcgggttcc gggtggagcc
     3421 gcgcatcaac ctgatgttcc gcaacgtggt cgaagacatg gtgaagaact tcgaagtcga
     3481 catcacctcg cgctacgagt ccctcagccg aaagaacatg atcggggatt tccgcttcgt
     3541 ggtgatggag aaattcctct cgtatgagaa tgagctatcc gcgtacgaga agatcatcat
     3601 ggacatctat ttcttcttca agcggatctc cttgtccgaa ccccgggcct tcggtctcga
     3661 caccagttcg gtgaccgtgg agaaggtgcc gatgatcgtg aatccgatcg ggcggttgtc
     3721 attgcggagg attacgtaag cgaacctttg gtttgacagc gatcggttgt gccggttgaa
     3781 agttgaaggg gaaaaagagc agcagccgta ttcgagacgc cttggataat aaagttgaag
     3841 gattccggta gtcgacgctc accggggatc ataacctact tgtgtagtcc gtgatttcac
     3901 gggtcattaa agccgatcag aaaaaacaat tagtgcttga agaatttttt acgatagacc
     3961 ctgtctccgg cctccagcgc catcaaatac aagccctcgc tccaatcagt caacgaaacc
     4021 gttgtttttc cctgtacggt ttcctgatag accagtttcc ccagcacggt gtaaacgcga
     4081 attgtaaaaa cgctgcttaa atctgaagca acattcaatt cttcgcccgc cggattcgga
     4141 aagactgaaa atggatttgt atcgaattcc tcgcgcaggc cggtaggggc ggagtaagtt
     4201 cccgttttgt accggacggt accactggcg ttgtcctgcc ataccaccca gacccgactg
     4261 tttttcagtt tcacgtccgc attggtgaca ttgttcactg caacggtgtc ataagacgcg
     4321 gggaaaccat tggtaatatc gggagttagc agaataggaa gttgagcatc accattcacc
     4381 gattgcttcc atacgatggc gatggctgac ccatcgtttg caatgcgcgg atagttttgt
     4441 tgactcagtc cgggaatgtt tccggtgagt ggttcaatgc tgcagagtgt attggagcca
     4501 aggcaggttt tgctcaagta ggcctggtag cttccggttc ctccgctcat gaaggtggca
     4561 tacagggtgt cccccacgat gaccccatcc ggcccgctgg ccggacaggc tgcgatcacc
     4621 caattgttat catctaccgc acaaccgttt gtaaaagaaa tggcattatc cgctgaaatg
     4681 cccgcccagc tatcccggag attggcgttg ttgtcgcgat agagcatcac acaagtattg
     4741 ccctccgaga tgatctcacc cggacaacaa tcacacacct ccgccgagcc accccaaccg
     4801 ctggccttcg tatcagtggt gaacgtgtta ccataatccg aggaacgcag gacagcccag
     4861 cggctatcca gaaatgacga attgaatttc atgaatgcaa caatgggatt gccggtcgca
     4921 tcggttgtaa cggccggaaa gcgtgagatg ctgtcggcga tgaatccgag ttccaccggg
     4981 ttgctgaacg acacgccgcc gttgaatgaa gcaaacaaga agatacgatt agagtccgcg
     5041 gattcgggaa tgcgtttaac cacaacatac acggtatcac catgtgaagc gatatcggga
     5101 cccatccagc ttgccgtagc caccggcatc cagggcggat tgagtatcac cggagcactg
     5161 aacgcggacc ccgtccaccg ggaaaagaaa acggtctgat cgttcattct tccccaaacc
     5221 accaaaggat ttcccgcccc atcgaggcaa agtcgcgggt gcatgttgct gcaggtatcc
     5281 ggcgcaacat ctccggaagt cgtccaggtt agttgcgcag tggcagatac gtgaaacaat
     5341 aagatgaacc aggctaacca gtatttcatt gcgcgaacgt gtataagatt aaagggggaa
     5401 gacaagcatt tgatttcaag ataaagatcg cacatttctt tataagaatt aggcagggaa
     5461 cgaccacccc catttcaaaa aattgaagga ggtctatttt aaatgaaagt actttccttg
     5521 atcgagaaga cctttgtgga agcctctttc tacttaaaac gaatcatgct tccccaacga
     5581 cggtcataca gtcttgtcac cagcgcggta agtggtgaga aagtgccgat gatcgtgaac
     5641 ccaatcgggc ggttgtcgtt gcggaggatt ccctgagtag gactgggcgc ggccccgctt
     5701 caacagggat caggtgtcgc gcccgagggt gtgctccgcg attgcaaaga gattctgatc
     5761 ccgcatacac cggaaatgtc cctttggaca cttgccgtat ccgatcttgg agcagggacg
     5821 gcaagagagg ttcttgacct cttcgatgaa aatcggttcg gtgcttccct gttttttgta
     5881 aggatacata ccgaattccg gaatggtgtt tccccagacc gatacgatcc gcttaccgaa
     5941 ggctgctgcg atgtgcatca gccccgtgtc gtgggtgatg atggagtcgg catgtttcac
     6001 gagcgcggcg gaggcgttga gtccgctttt gccgcaggca ttgaagaccg tttctccgac
     6061 gcggtgacga atgaattcgc ccagctcggc atcttccttt ccgcccagta acacgaccgg
     6121 acgcttgagc atgccgcaaa gcgttaccag gttccgatcg ggcattcgtt tggtctcgtg
     6181 tcgcgcaccg atgacgaagc cgatgtagcc gttgcggtgg gagggaggca gtatcgaaag
     6241 cccttcctgt tcctcgtctg cgctgatgaa gtagtcgagc cccttaccgt cgttgcgcac
     6301 cccgaaacct gcgacagttt ccaggtaacg atcgactatg tgaagcttgg gcaggcgatc
     6361 caccttcagg ttgacgcgca accatttttc cacgttgagt tttcgaaacg cgtagcgctt
     6421 caccccaagg gcggctgcaa cccggagggt acgcaggttg tggtgcaggt cgatgatggc
     6481 atcgaaacgt tctgctttca gctcacgcaa gggcagttga tcacctgttc cggtaaggtg
     6541 taaacggtcg atgtatggat tgtgctgcaa taccggttcg aaggagcgct tggtaagaaa
     6601 gtgtacctcc gcgccgggca attgctcctt caggcaacgt atcaccgggc tcgtgagcac
     6661 gatgtcgcca atggaagaaa accgaaggag cagaaatttt gacacgcagc attcaaagat
     6721 accgatccgc cggaattcga cttggaagaa aggatgaagc cggaatttcc gtcctttgtc
     6781 atcccgaatg ttactggtcg atacccatac ccacctctac gccgacgctt ttaatgccga
     6841 ccgtccggac ctgatcaaag ccgcgatggc aaagggcatc ggacgatttt acctgcccaa
     6901 catcgatgcc gcatccatcg gtccgatgct cgaattggaa gccgcttacc ccggtgtctg
     6961 catcccgatg atgggcttgc acccctgtca tgtgcagcag aactggcagg cggcgctgaa
     7021 aagggtagaa gcggagttgg tgcaccgaaa atgggcggcg atcggggaga ttgggatcga
     7081 cctgtactgg gacaaaacct attttgccga gcagcagcag gcatttttgc aacaagtggc
     7141 ctgggcgaac gaacgacagc taccggtcgt tatccactcc cgggagtcct tcgcggaaat
     7201 catcgagctg ctcgaccggc agcccaaggc atcgccgcaa gggatcttcc attgcttcac
     7261 cggaacgatc gaacaagcgc gacaagcgat cgaccgcggt ttttacctcg gcatcggagg
     7321 tgttgtcacc ttcaagaaat ccggattgga tgcggtggtg gaacaaattc cgctcgaaca
     7381 cctcgtactg gagaccgacg ctccctacct tgcaccgacc ccgcaccgcg gcaagcgcaa
     7441 cctgcccgag tacgtctatc tcgtagcgga aaagatcgcg gagatcaagg gtgtgacgat
     7501 tgaagaggtg gctaaggtga cgacggagaa tgccaacagg atattcggga tatgaaatgg
     7561 gggttgaaag tttcgagttc cgagtttcct gccttcgctg aagcttcggc aggcaggcga
     7621 gtttcgggtt ccgagtttgg tattgttaat tcctgattag ggttgaaagt tgcagattgc
     7681 aggttgaaaa agatatttca tttagaacca cagattacaa tagaaagcgg aaaacccaag
     7741 accataagtt aaacgcgaaa cccggaaccc ggaacaaaat agctcttcgt ccctcgcccc
     7801 atgtcccgca agacaacctc atcccgcaaa aagatcctgc tcatctatac cggaggaaca
     7861 atcggtatga tgaaagacca ccataccgga gcactgcggc ccgttgattt cagcgcgatc
     7921 gttgaacaag tgcccgaact gcgtcagttg aattgcagct tcgggtttca tgctttcggc
     7981 ttgcccatcg attcttcgaa tgtccagccg gaattctggg gtacccttgc gcgggtgatt
     8041 gaacgcgagt acgcgcgcta cgacggcttc gtcattcttc acggcaccga cacgatggcg
     8101 tatactgcat ccgcgctgag ttatatgttg cagaatctgg acaagcccgt gttgttaacc
     8161 ggctcgcagt tgccgttagg cgccattcgt accgatgcac gcaggaacct catcaccagc
     8221 gtggagatca tcgccggcga tgtactcgta ccggaggtgt gcatctgctt cagcaatcaa
     8281 ctctaccgcg gcaaccgtgc ggagaaatat acatcttcga aattcgatgc attccactcg
     8341 ctcaactatc cggtattagc ggaagcgggc acacacatcg tcttcgacaa accggagatc
     8401 cggaagaaac ccaccggaaa atttcgggtg aaatatggtt tcaataccga agtggcgttg
     8461 ctcaagatct atcccggcat ccacactgaa gccatgcgag ccattctttc tacacccggt
     8521 ttgcgcgcgg cgatcatcga aacctacggc agcggtaatg cgccgacgga tcccggattc
     8581 ctgcgcgtcc tgaaggaagc gatcgaccgc ggcatcaccc tcatcaacat ctctcaatgt
     8641 tcaggaggat cggttgagca gggaaaatac gaaaccagcc tgcacctgca ggagatcggc
     8701 gtagtcagcg gcatggacat gaccaccgaa gccgcgatta ccaaagccat gttcctgctc
     8761 ggacaaggct acaaaggaga tcggtttgag cgggcaatgg taaaaagcct ggccggggag
     8821 gttggggttt tctagtttct agtttctagt ttctagtttc gggtttcctg tttcctgttt
     8881 cctgtttcct gtttcctgtt tcctgtttcc tgttaatacc acca
//
LOCUS       DAOTON010000013         8399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_7616_length_8399_cov_3.889142, whole genome shotgun sequence.
ACCESSION   DAOTON010000013 DAOTON010000000
VERSION     DAOTON010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7616_length_8399_cov_3.889142"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3472)
                     /locus_tag="P5292_00540"
     CDS             complement(<1..3472)
                     /locus_tag="P5292_00540"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRS37641.1"
                     /translation="MRLTLREKALRVLLIIALSVPAFAEAQVVINEICPSNSTGLTND
                     DGDKDDWIELYNKNNSSVNLNGYGLSDDPTQLDKFIFPSTTIPAHGRMVIFASDRNKT
                     ELVDHWETAVNASTQWRYAVGSNSIDTNWRNLGFNESAWSQGQGGIGYGDGDDQTSVA
                     SGTRSVMMRKTFNVPDTSDIARIVFQMDYDDGFVAYLNGVEIARVNLGVAGVRPAWNE
                     LAPGTMEAKIYQGLQPDSFNLDMSFIRTILRQGNNVLAVEVHNQSTSSNDLTARPFLS
                     FGLKSPGSTYGSLPSWFRVPPTDYYNADFKLSRTGETVYLTDGSRSVIDQQAYTNIEL
                     DNSWGRSSDGSGSWCLFGTPTPNASNSGSSCYSGYASIPIFSLSPGFYPSTRWLNLTT
                     TTPGGQIRYTTNGDDPTTSSRLYSSSVLIDTTQTVRAKVFAPGMLPSQTITNSYFIDE
                     DIDLPIWTITTDYDNLWDYNTGIYVMGPGASSSSPYFGANFWQDWEKPAAIEYWDKDK
                     NRLLRFNADIEIYGNYSRAKPQKSFNIKLSDKYGTGEINLPMYSTKPWLDKTDDIVLR
                     NSGTDWNKVHFRDAFMERGLKSTYSGYLAADPVVLFLNGEFWGVYTNHENHDGNWIGN
                     NFGYKSDEIDYLKEDGSTMTIKEGSDTSFWALYNYATTQSPSTQAYYNYIDARLDLKA
                     YADYFIAETYYNNGDWIGDWTNNIKLWRPREAGGKWRYLVYDLDFGCGLSGASPTENR
                     LGMALNPSAFSNSSNMFDAITDNPTFKNYFINRYADLINTIFLEDSLDNIIQEYESEM
                     SHDMQQHFQQWGSTNSDWQDYIDEAETFIRRRAAYARDQIESEFGLTSQVTLTLQVDP
                     PGAGRIEISTITPATYPWSGVYFRGNNVTVTAIPNPGYTFDEFSSTTLGSHNHNQSAN
                     ANFTSSQTITARFNGSSRTPDLDISEINFNSDSAYDAGDWVEIKNNGNYEIDLSGWSI
                     RDAQDNHRYVFPTGTTIPANGYLVIAEDMEKFDAQHPSVANVIGPTGFNFSNSEDQIR
                     LSDWRDDLVLMLYYQDVAPWPSGADGDGYTYERANFSGDPSDGNNWIVGCIGGSPGQA
                     FVPLNTTVNFSGSTLLCTGQSTQLTVSASGTGNTYQWRRDGVILPGETNATITITDAG
                     EYDVLVQRGGC"
     gene            3775..4428
                     /locus_tag="P5292_00545"
     CDS             3775..4428
                     /locus_tag="P5292_00545"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRS37642.1"
                     /translation="MIMGNSTARAAENLVPHTPEFTLPPLPYAYDALEPHIDKMTMEI
                     HHTKHHQAYVTNLNKALSETTLHADSLEEILSKVSGLSPAIRNNGGGHWNHSFFWKLM
                     KPNGGGAPAGKVAEAINQAFGSFADFQKQFNEAAMKRFGSGWAWLLVNGDGKLQIAST
                     PNQDNPLMDVSDTKGKPVLGIDVWEHAYYLKYQNKRADYVGAWWNLINWDQVAANLG"
     gene            complement(4429..8202)
                     /locus_tag="P5292_00550"
     CDS             complement(4429..8202)
                     /locus_tag="P5292_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HRS37643.1"
                     /translation="MKRIIVLIPALLFSLSLLAQRNKNIPPTGSITCSEFAISRPLSE
                     IAKEFPVDESKPYEKEESEDRENRKPQRFKFTVADGPEYGNDPATIQTKMGTVGTRAP
                     LTSWPGQTATGFRPYDPSGAAGPSHYVQMINSTTFKVYNKTTGSVILTATLGNLWSPA
                     TGNAGDPIVLYDKAADRWFLAQFGTSSDRKIYIAISTTGDPTGTYYTYTFVSPAFPDY
                     LKFGVWADGYYMTSNQAQKVFAFERTAMLAGNAASRAVYQSFSPPQSSGFFCPLPGDA
                     ADGTLPTVGAPCPIFSYSDNGWGAGYSDAVNIYQMSVDWVPTTPTATITLAANVATAT
                     FDASYNSSWNDVSQPGTTQKLDGIGGVCMYRAQWKSWSGYNTIVLNWAVNISATQRSI
                     KWCELRQDQSTGSWSMYQEGIYLPDAATRWMGSIAMDDNGSIGMGYIKTDASAGIYPG
                     LYYTGRRSCDPLGTLPITESLVIAGTGSQTGVNRVGDYAQLSMDPDGITFWYTGEYMG
                     GTTGGSAARTHIYSFQLPNCSNAAAVFIAQTSGSNPMCPGASATFTATPTNGGSAPVY
                     QWKVNGTNVGTNSTTYSTSSLVNGDVVTCVMTSNQAGVTGNPATSNAITMTVNSVSTP
                     SVTIAQTAGTNPICAGSSATFTATPVNGGTTPSYQWQVNGSNVGTNSGSYSSSSLTNG
                     QTVSCIMTSNALCVTTPTANSNTLTVAVQAVGDPVVTIAQTGGTAPACAGATLTYTAT
                     VTGGTPTFYQWKVDGANAGASASTFSSAGLGNGQTVSCTVTATPTCPVLTYKNLGTAT
                     TTNASTSSLGAAYPTYYGNGRQQYLIRASELTALGLSAGNMASIGFTTGTTLGDPATL
                     NGYTIKIGTTTATAASATFLAPTFTTVYGPVNYTPTLSATNTHVFNTPFTWNGTSNLV
                     VDICFSNQVVGNAAYATRYSSPGFNASAYYQADGAGGAGACTQATGATSTRRPNMVIA
                     MANPTAMVNSNVLTANISALHSISGFAPTSGGSGTSVVITGTGFVNVTSVLFNGTPAT
                     SYTVNSPNQITAIAPAATTGPVTVLTSACGAVNAAGSFNYGSGLTLNLKLYIEGFYLG
                     GGQMIGVLSPSVTDTVTAELASASPPYTILHTTTGTVSTAGNASFLFPSAILGNSYYF
                     VVKQRNTLETWSASPVLCNVSPITYDFSTGAATAYGSNLSDLKDGRYGIWSGDVNQDG
                     LIESTDYSGIENAVTTFLYGYVPEDLTGDNIVEASDYSLVENNALLFLFAVRP"
BASE COUNT         1930 a         2169 c         2341 g         1959 t
ORIGIN      
        1 tgcagccacc gcgctgaacc agtacgtcat actcacccgc atcggtgatc gtaatggtcg
       61 cgttggtttc tccgggtaga ataacgccgt cacgccgcca ttgatatgtg ttgccggtac
      121 cggatgctga aacggtcagt tgcgtggatt gaccggtaca caacaaggtg ctgccggaaa
      181 aattcacggt ggtgttcagc ggaacgaatg cctgtccggg cgatccgccg atacaaccga
      241 cgatccagtt gttaccatcg ctcggatctc cgctgaagtt ggcacgctcg taagtgtaac
      301 cgtcaccgtc ggctcccgac ggccagggtg cgacatcctg ataataaagc atcaggacaa
      361 ggtcgtcacg ccagtctgaa aggcggatct gatcttcaga attgctgaag ttgaaacccg
      421 tcggtccgat cacgttcgca accgaaggat gctgggcatc gaacttttcc atgtcctctg
      481 caatgaccag gtagccattt gcagggatcg ttgtaccggt cgggaaaaca tagcgatggt
      541 tgtcctgggc atcgcgaatg ctccagcccg acaggtcgat ctcgtagttt ccgttgttct
      601 tgatctctac ccagtcgccc gcatcgtaag cggagtccga attgaaattg atctccgaaa
      661 tatcaaggtc gggagtccgg gaagaaccgt tgaagcgcgc ggttatggtc tgcgaactgg
      721 tgaagttcgc gttggcgctt tgattgtgat tgtgactacc aagcgtcgtt gaggagaatt
      781 catcgaacgt atagccagga ttcggaatgg ccgtcaccgt tacattgttg ccgcggaaat
      841 aaacgccgga ccagggatag gtggcgggcg tgatcgtact gatctcgatc cttcccgcgc
      901 cgggaggatc cacttgcagg gtaagcgtca cctgcgaagt caatccgaac tcgctttcga
      961 tctggtcgcg ggcgtacgcg gcgcgcctgc gaatgaacgt ttctgcttcg tcgatatagt
     1021 cctgccaatc ggaattcgta cttccccact gctggaaatg ttgctgcata tcatgcgaca
     1081 tttccgactc atattcctga atgatattat caaggctgtc ttccaggaag atcgtgttaa
     1141 tcagatccgc atagcggttg atgaagtaat tcttgaacgt cgggttatcc gtaatggcgt
     1201 cgaacatatt cgacgaatta ctgaaggccg aggggttaag cgccataccc aggcggtttt
     1261 cggtaggaga agcaccactt aaaccacaac cgaagtcgag gtcatagacc aggtagcgcc
     1321 atttgcctcc tgcttcccgc ggccgccata acttgatgtt gttcgtccag tcaccgatcc
     1381 agtcaccatt gttgtaatac gtctcggcaa tgaagtagtc cgcataggct ttcaggtcca
     1441 ggcgcgcatc gatgtagttg tagtaggcct gtgtagacgg gctctgcgtt gtggcgtagt
     1501 tatacaaagc ccagaaggat gtgtcggatc cttctttgat cgtcatggtg ctgccatcct
     1561 ccttcaggta gtcgatctcg tccgacttat agccgaagtt gttaccgatc cagttaccat
     1621 catggttctc atggttggta taaactcccc agaactcgcc gttcaaaaag aggacgaccg
     1681 gatcggcggc gaggtatccg gaatatgtgc tcttcaagcc acgctccatg aacgcgtcgc
     1741 ggaagtgaac tttgttccag tcggtacccg agttgcgcaa gacgatatcg tctgtcttat
     1801 ccagccaggg tttggtggag tacatcggca gattgatctc gccggtgccg tatttgtcgc
     1861 tcaacttgat gttgaaactc ttctgcggct tcgcccggct atagttaccg tagatctcga
     1921 tgtccgcgtt gaatcgcagg agacggttct tgtccttatc ccaatattcg atggctgcgg
     1981 gcttttccca gtcttgccag aagttggccc cgaaataggg actcgaactg ctcgcaccgg
     2041 ggcccatgac gtaaatgccg gtattgtaat cccacaggtt atcataatcg gtcgtgatcg
     2101 tccagatcgg caggtcgata tcctcgtcga tgaaataact gttggtgatc gtctgactcg
     2161 gcaacatccc gggagcaaac acttttgcgc gaacggtctg cgtggtgtcg atgagcacag
     2221 aggagctgta caggcgagaa gatgtcgtgg ggtcgtcgcc gttggtcgta tagcggatct
     2281 gtcctcccgg tgtagtcgtc gtcagattca gccaacgggt gcttggatag aatccgggcg
     2341 acagcgaaaa gatcgggatc gatgcgtaac cactatagca ggatgagccg gaattactcg
     2401 cattcggagt aggagtacca aaaaggcacc aggagcccga accatcggaa ctccggcccc
     2461 aggaattgtc caactcgata ttggtgtatg cctgctgatc aatgacggaa cgcgaaccat
     2521 cggtcaggta aaccgtctcg ccggttctgc tcagtttgaa atcagcattg tagtagtcgg
     2581 tcggcggaac ccggaaccag gacggcagac taccatacgt cgagcccggc gacttcaaac
     2641 cgaaagaaag gaaaggacga gcggtcaggt cgttcgagct ggtagattgg ttgtgcactt
     2701 caaccgcaag gacgttgtta ccctgccgca ggatcgtgcg gataaaactc atgtcgaggt
     2761 tgaaggaatc cggctgaaga ccctggtaga tcttcgcctc catcgtaccc ggagcaagtt
     2821 cgttccaggc cggacgaaca ccggcaacac ccagattgac gcgtgcgatc tcgactccat
     2881 tcaggtaggc cacaaatcca tcatcgtaat ccatctgaaa cacgatgcgt gcgatgtcgg
     2941 aagtgtccgg cacattgaat gtcttccgca tcatgacgga gcgcgtaccg gacgcaaccg
     3001 atgtttgatc gtcgccgtcg ccgtagccga ttccaccctg gccctgagac caggcacttt
     3061 cgttgaagcc gagattacgc cagttggtat cgattgaatt ggaccctacc gcgtaacgcc
     3121 attgcgtgct ggcattcaca gccgtttccc aatgatcaac gagttcggtt ttgttgcgat
     3181 cggaagcgaa gatcaccatg cgaccgtgcg cggggatcgt cgtgctcggg aaaatgaact
     3241 tgtccaactg ggttggatca tcgctcaagc cataaccatt caggttgacc gacgaattat
     3301 tcttattgta aagttcgatc caatcgtcct tgtcaccatc gtcattcgtc agaccggtgc
     3361 tgttggaagg acagatttca ttgataacca cctgtgcttc agcaaaagcc ggaacggaca
     3421 gggcaataat caagagaaca cgaagagcct tttcgcgtag agtcaagcgc atgtgagggg
     3481 ttgagagagg tgtgtttcta tggatgcaaa gtggcaaatg agaatcttca tctcagtcac
     3541 aaaattaaac ggaaatgacc tcctttttaa gcttttttga cgaatgatta caaagactgg
     3601 gtggaaaaag ggctcctgac ggacaatgtc atgtcatctc ctccaaattc attaaaaatt
     3661 cgcaaaaaat ggggaccttt aaaggagatt aaaattatca acaacatgtc cagcaaccgt
     3721 cgtgaattcc tgaaaaccgc gtccatggcg gctttcgcta gccttggcac cagtatgatc
     3781 atgggtaatt caaccgcccg ggcagcggaa aacctggttc cgcacacccc ggaatttacc
     3841 ctgccgccac tcccctatgc ttacgatgcc ctcgaaccgc acatcgataa aatgaccatg
     3901 gaaattcatc atacgaagca tcatcaggct tatgtgacga atttgaacaa ggcgctttcg
     3961 gaaacaacac tgcacgcgga tagtctagaa gagatcctct ccaaggtttc gggtttgtct
     4021 ccagcgattc gtaacaatgg cggcggacat tggaatcatt ccttcttttg gaaattgatg
     4081 aaacccaatg gcggcggcgc tcctgcgggt aaagtcgcgg aagcgatcaa ccaggcattc
     4141 ggtagcttcg ccgactttca aaaacaattc aacgaagcgg cgatgaaacg cttcggcagc
     4201 ggctgggcct ggctgcttgt gaatggggat ggaaaactac agatcgcttc cacacccaat
     4261 caggataacc cgctcatgga cgtatccgat acgaaaggaa aacccgtatt gggcatcgat
     4321 gtttgggaac acgcttatta cctgaaatac cagaacaagc gagccgacta tgtcggtgcc
     4381 tggtggaacc tgatcaactg ggatcaggtt gcggcgaatc tgggatgatt atggcctaac
     4441 ggcaaagagg aacaacaagg cattgttctc caccagactg taatccgaag cttcgacaat
     4501 attgtctccg gtcaggtctt ccggcacata tccgtacagg aatgttgtaa ccgcattctc
     4561 gatcccggaa tagtcggtgg attcgatcaa accatcctga ttcacgtcac cgctccaaat
     4621 gccgtagcga ccatccttca aatcggacag gttgcttccg taagctgttg cagcacccgt
     4681 agagaagtcg taggtgatgg gagaaacatt gcacaagacg ggcgaagcgc tccaggtttc
     4741 cagcgtgttc cgttgtttca ccacgaagta gtacgagtta cccagtatgg ccgaagggaa
     4801 caagaagctg gcattcccgg cggtggacac cgttccggta gtggtatgca ggatagtgta
     4861 aggaggcgag gcggatgcaa gctccgccgt caccgtatcg gttaccgacg gtgacaacac
     4921 cccgatcatt tgaccgccgc ccagatagaa tccttcgatg tataacttaa gattcagtgt
     4981 taatccgcta ccgtagttga aactaccggc agcattgaca gctccgcagg cactggttaa
     5041 cacggttacc gggccggtgg tagctgccgg cgcgatggcc gtgatctggt tcgggctgtt
     5101 aaccgtataa cttgtcgccg gtgttccgtt gaacaatacg gacgtcacat ttacaaatcc
     5161 ggttccggtg atgacgacgc tggtgcccga acccccgctt gttggcgcaa agccactgat
     5221 cgaatgcaat gccgaaatgt ttgccgtcag cacattcgag ttcaccatcg ctgtcggatt
     5281 ggccatggca atgaccatgt tcggccttcg ggtggaagtt gcgccggttg cctgcgtaca
     5341 cgcgcccgcc cctcctgccc catcggcctg atagtaggcg ctcgcattga agcccggaga
     5401 actatagcgg gtggcgtagg cggcattacc gactacctga ttggaaaagc aaatgtccac
     5461 aacaaggttg gaggttccgt tccaggtaaa aggcgtgttg aatacatgcg tattcgtggc
     5521 gcttaaggtc ggggtgtaat tcaccggacc ataaaccgtg gtgaaggttg gagctaggaa
     5581 ggtagcagat gctgcggtag cggtcgttgt gccgatcttg atcgtatacc cgttcaaggt
     5641 tgccggatca cccagtgttg taccggttgt gaatccgatc gatgccatat tgccggccga
     5701 aaggcccagc gcagtcaatt cgctcgcccg gataaggtac tgctgtcggc cgttaccgta
     5761 gtaggttggg tacgccgcac ccaggctgct ggttgatgcg ttggtcgttg tggcggtacc
     5821 gagattctta taggtcagga cgggacaagt gggtgtcgcg gtaaccgtac agctcaccgt
     5881 ctgcccattc ccaagtccgg cactggagaa ggtcgaggcg ctggcgcccg cattcgctcc
     5941 gtcaactttc cactgataaa atgtaggcgt gccgccggtt acggtggccg tgtaggtaag
     6001 tgtagcgccc gcacaggccg gggcggttcc gccggtttgt gcgatcgtca ctaccggatc
     6061 gcctactgcc tgcacagcga cagtgagggt attcgagttc gccgtcggag tcgttacgca
     6121 gagtgcgttc gaggtcatga tgcaactcac tgtttgcccg ttggtcagcg aactcgaact
     6181 ataactgccg ctgttcgttc cgacattgga accattcacc tgccattggt aggatggcgt
     6241 agtacctcca ttgaccggcg ttgccgtgaa agtcgccgaa gaaccggcgc agatcggatt
     6301 cgtgccggcc gtttgcgcaa ttgtaaccga cggagttgac acgctattaa cggtcatggt
     6361 gatcgcattc gaagtagcgg ggttgccggt gacaccggct tggttagagg tcatgacaca
     6421 ggtcaccaca tcaccgttga ccagactact ggtggaatac gtggtactgt tggtgccaac
     6481 gttcgtgcca tttactttcc actgataaac cggggcgctg cctccattcg tcggggtggc
     6541 cgtgaaagtc gccgacgctc ccggacacat cggattagag ccacttgtct gtgcaatgaa
     6601 tacagcagcg gcattcgaac agtttggcaa ttggaacgag tagatatgtg ttcgggcggc
     6661 cgatccgccg gtagtcccgc ccatgtattc accggtatac caaaaggtga tcccgtccgg
     6721 gtccatcgat aactgagcat aatcacctac ccgattgaca ccggtttgag atccggtgcc
     6781 cgcgatgacc agcgattcgg tgatcggaag ggttcccagc ggatcgcagg agcgacgtcc
     6841 ggtataataa agtccgggat atattccggc cgaagcatcg gttttgatat aacccatgcc
     6901 gatggatccg ttatcgtcca tcgcgatgct gcccatccaa cgagttgcgg cgtccggaag
     6961 gtaaattcct tcctgataca tggaccaact cccggtagat tggtcctgtc gtaattcaca
     7021 ccatttgata cttcgctgcg tggcactgat gttgacagcc caattgagaa cgatggtatt
     7081 gtaaccgctc caggacttcc attgcgcccg atacatgcaa accccgccga tgccatccag
     7141 cttttgtgtg gttcccggtt gcgatacgtc attccagctg gagttgtacg aagcgtcgaa
     7201 ggttgcagtc gccacattcg ccgccagcgt gatcgtggca gtcggtgtgg tgggaaccca
     7261 gtcaacagac atctgataaa tgttcacggc atcggaataa ccggcgcccc aaccgttgtc
     7321 ggaatatgag aagatcgggc agggtgcgcc aaccgtagga agtgtaccat ctgcggcgtc
     7381 accgggcagc ggacaaaaga aaccggaact ttgtggcgga gagaagctct gataaaccgc
     7441 acgggaggcc gcgttaccgg ccaacatggc ggtcctttca aaggcgaata ccttttgtgc
     7501 ctggttggag gtcatgtaat atccgtccgc ccatacaccg aacttcagat aatccggaaa
     7561 tgccggtgaa acaaacgtat acgtgtagta ggttccggtg gggtcgccgg tcgtggaaat
     7621 cgcgatgtag atcttcctgt ccgaactcgt gccgaactgc gccagaaacc aacggtctgc
     7681 ggctttgtcg tagaggacga tcggatcgcc ggcgtttcct gtcgccggcg accaaagatt
     7741 tcccagcgtt gctgttaaaa tgacgctacc ggtagtttta ttgtaaacct taaaggtggt
     7801 cgagttgatc atctgcacat agtgcgatgg tccggccgca ccggaaggat cgtagggacg
     7861 aaagcccgta gccgtttgtc caggccagct ggtcagcggt gcgcgcgtgc ctaccgtacc
     7921 cattttggtt tggatggtag cggggtcgtt cccgtactca ggaccatccg ccacggtgaa
     7981 cttgaatcgc tgcggttttc tgttctcgcg atcttccgat tcctcttttt cgtagggctt
     8041 actttcatcg accggaaact ccttcgcgat ttcgcttagt gggcgactga tcgcaaattc
     8101 actacaggtt atggatccgg ttggtggaat gtttttgttg cgttgtgcca gcagggatag
     8161 gctgaataac aatgcaggaa tcagaacgat gattcttttc atggtgatgg tgataacggg
     8221 cgcagtccgt tgcgacgaat ggatgactac acgcggtatg cgcgcagtcc tatggagaaa
     8281 ctgagaataa gaatgccggt cggttctgct caggaatcgg acaggcaggt cgcagagagt
     8341 gacaaaaata acccgaatat gcaggcgatg caatgacccg gcaccttctt atgtgtgat
//
LOCUS       DAOTON010000014         8361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_7651_length_8361_cov_2.592824, whole genome shotgun sequence.
ACCESSION   DAOTON010000014 DAOTON010000000
VERSION     DAOTON010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7651_length_8361_cov_2.592824"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1583
                     /locus_tag="P5292_00555"
     CDS             159..1583
                     /locus_tag="P5292_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRS37644.1"
                     /translation="MSHSNRFQPNRAVFALLVLLATAFQSMAGPDAWVTKTIVGWNGS
                     SRYTFLRDSSLYTEGWDTVGQARFWREVITMTSDTCIINIASCRKPVERVSRSVWMNQ
                     TELEKICFKDSLSQVHCIAPTEQLYVTAGKREFYEVKKTLDDINQAIQVFRKHGCDPF
                     YAQAILLIESPGKGAARSYVGARGPFQLMPAVARRFGLKVSKYHDDRLDMNKAGMAAA
                     RLINTGCVPYIRKFLDEKGIPYQEHDLWFRLLVLHAYHAGAGNVRCVINALDPKAGGL
                     QLFEQIWQTTCGGFKNESQNYSQIALASLMNFDELIEQDGDTVFLVKGDRMLSRYSRA
                     GMKPWEAYEYLQASLRAYEHDLIDDMIPYEYFIRQVGQIRKEFTHWASVITLASRDVI
                     LKPYPASEEHVSLLAGELSKRRRFDEAIKLLKLNLDMHPGSPALYDSLAKAYRKSGDQ
                     KMADLYAGKAQAAAALQPGDKPNE"
     gene            1805..4087
                     /locus_tag="P5292_00560"
     CDS             1805..4087
                     /locus_tag="P5292_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37645.1"
                     /translation="MNLDQRQALPNGDIVLVTDSTHPYGAGYSWTWPDSLPSRSVLLH
                     CEAKLRSSTLNPGIQWVLSIEEQGQTLLWDGRDLSNQLGKQDEFINTNVGFKLPANFL
                     RRGTTIKVYLYQPDRRSRVETSGVTFSLCRWGLLSFLPPGSPQSDSRSAEQELFLGTD
                     YAVRIQPAGGILRVDRPDGQRLIRNVLLYTEAKGDSITPRFSTLEFQRDSVAQGVHHL
                     LFRSANERFNTTITLATDEKAAAIRWSLNTEWLQPTELVRQAIVMLVQPAASQAVDVV
                     GRMLRAGDDEESWLGRGAVSFGDSVPEMIVSYPDQLSSLQLDWASNTLWLNADWSMDH
                     PLLHWPKEKKSRNHRLDRSCSVYKVHERLEARWQTYFRMPSFLPALQDRPFGYDATFI
                     WTEHADYSDLKLQRAVMFGHDSISDPSTAKKGFVARGIPMTKSVFYNNPDRVTNRKRH
                     AAFPGTIATMRETPGFEALMQTLEESGSEIALHTPEQFTSNRKQLDVALQYMRSRFNT
                     TTWIDHGYDNAPTSNREDLVCDGLDSLSDGYALDTWLRYGITSVWNCFYEDTNVFLPY
                     SFDSRLDAPYSGISGRFPAPVWWTHPTRSKSILHWRTNTTLDPPDAGMWSYYLGEQRL
                     DDLVRRRGTYIAHCYPARVDSTNGFYRLNGGHWEIEPAFDDALALQARYREEHKLWLP
                     VVREYLSYQQRIREVKMVPTEDGWRYSNSGHQPVQGFTLYSEHLLDAGSKAMQIRSSG
                     SGHYYSFNLDPGETVFIKLK"
     gene            4098..4823
                     /gene="mtgA"
                     /locus_tag="P5292_00565"
     CDS             4098..4823
                     /gene="mtgA"
                     /locus_tag="P5292_00565"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HRS37646.1"
                     /translation="MAGKKVTGKGKSGWWARLRKWTIRIVAGFFILSILSVILFRFVP
                     VPVTPLMLIRCVEQLTDGRSLRMSKDWTSLDKMSPALPLAVIAAEDQRFEEHFGFDFD
                     AIQKAQRYNERHKGKRLKGASTISQQTAKNLFLWPSRSYVRKGFEVYFTFLIEVFWSK
                     ERIMEVYLNIVEMGEGIYGAEVAAKEYFHKPAARLTNREAALLAAVLPNPRKWSPARP
                     TAYIQRKASRIVRFMGNLELEDF"
     gene            4903..6327
                     /locus_tag="P5292_00570"
     CDS             4903..6327
                     /locus_tag="P5292_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37647.1"
                     /translation="METAQFSNDRLKYFLFFLAVLLSRLPFISDGYGLDGDSWAVALT
                     ARNIHDTGSYEASRLPGYPVQELLSALLINGGATALNLLVAMVSAAGILFFALSLKVL
                     RFRHPLLAAAALAAVPVIYIHSTTTIDYTIALGFLLASFYFVVSEKPVVAGILTGLAI
                     GTRVTSGAMLLPYIIILIGNGGLFVNLRRILLYAVPALIVGLLCFAPVIQRYGWDFLT
                     YYPVPYPSIAKILYKFTFEVWGVVGLFAIFLGVVMLFLPNRITSKRYLFPRSVNERYV
                     IAWLIAIDLYVIAFLRLPMESGYLAPVVPFVILILGKYLYEPAFKLCCYLLILSPFVG
                     SLGPVDRLDAAKPSSVSFRFRAGGETLVLDALRGPVVAYKSRRQEGMNFTREILNSVD
                     SISRPAVLVSGRWYNQLVVQEGDTTSGNLVFASYLDEPMLLYYIGKNYSVYYLPKQDY
                     YNQIMRNVDLNLFGAQAYLREEKY"
     gene            complement(6343..6828)
                     /locus_tag="P5292_00575"
     CDS             complement(6343..6828)
                     /locus_tag="P5292_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37648.1"
                     /translation="MLQPPAPSEGDRFIDRVFFGGNFGLQFGTQTIIEIAPIVGYRLT
                     DRLAAGVGGKYIYYRYRSAPFEYSTNMYGGSVFSRYNILENLFAYTEYEILNLDAPDP
                     LNPYRLRRTNVTSVFVGGGYRQMLGNNSSLNLMLLYNLNESTNSPYQNPIIRIGFGFG
                     I"
     gene            6968..7702
                     /locus_tag="P5292_00580"
     CDS             6968..7702
                     /locus_tag="P5292_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS37649.1"
                     /translation="MATLVITGGSSGIGFEATLWSAREGHTVMVVSRNRTRLGLLEAE
                     AEKRGVRARVHLLPADISKQQEVEAIGREIRSRSARIDALINNAGRLINKPFGELRAE
                     DWEEVYATNVFGTVSMSRVLLPGLIRSREALARGEAPGHIVNISSMGGVQGSAKFAGL
                     SAYSSSKAAIIGVTECLAEELKPRGVHVNALALGSVQTEMFSAAFPGIDASTTPERMG
                     EYVARFALEGMHLFNGKTIPVSASTP"
     gene            8300..>8361
                     /locus_tag="P5292_00585"
     rRNA            8300..>8361
                     /locus_tag="P5292_00585"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1902 a         2053 c         2332 g         2074 t
ORIGIN      
        1 cctttgaaga ggcgaccgat ctctgcatga actcgctttc acggcaaatc aaaaagcata
       61 aggagaaagt tcgcgggctc tgagctgaca attgcgtaag gatttcctgc gttttatccc
      121 aacttccgga acctccggac gtatgggtta ttcgaggtat gtcgcattct aaccgttttc
      181 aaccgaatcg agccgttttt gcgctcctgg tgttgctcgc cacggcgttt caaagcatgg
      241 ccggccccga tgcctgggtg accaagacga tcgtgggatg gaacggcagc agtcgttaca
      301 ccttcttgcg cgattcatcg ctttatacgg aaggctggga caccgtcgga caggcgcgct
      361 tctggcgcga ggtcatcacg atgaccagcg acacctgcat catcaacatc gcttcctgcc
      421 gtaaaccggt ggaacgcgta tccagatcgg tgtggatgaa ccaaaccgaa ttggagaaga
      481 tctgcttcaa ggacagtctc tcccaggtgc attgcatcgc tcccacggag caattgtatg
      541 tcacggcggg gaagcgggag ttctatgaag tcaaaaagac actcgacgat atcaatcagg
      601 cgatccaggt attccggaag cacggctgtg atccttttta cgcgcaggcg atcctgttga
      661 tcgaaagccc gggtaaaggt gcggcacgtt cctatgttgg agcacggggc ccctttcagc
      721 tcatgcctgc cgtggcccgt cgctttggat tgaaggtttc caaataccac gatgatcgtc
      781 tggacatgaa caaggccggt atggccgccg cccgcctgat caatacggga tgtgttccct
      841 atatccggaa gttcctggat gaaaaaggca taccgtatca ggaacatgat ctttggttcc
      901 ggcttttggt gttacacgct tatcatgccg gtgccggtaa tgtccgttgt gttatcaatg
      961 cactcgaccc caaggccggc ggcttgcagt tgtttgagca aatctggcaa acaacctgtg
     1021 gtggctttaa aaatgaatcc cagaattaca gccagatcgc gttggcttcg ttgatgaatt
     1081 tcgacgagtt gatcgagcag gatggagata ccgtatttct ggtgaaaggg gaccggatgc
     1141 tttcccgtta ttcccgtgcc ggcatgaagc cctgggaagc ctacgaatac ctgcaggcat
     1201 cgctgcgcgc atatgagcac gacctgatcg acgatatgat cccgtatgag tacttcatcc
     1261 ggcaggtagg gcagatcaga aaggaattca cgcattgggc atcggtcatc acccttgctt
     1321 cccgggatgt aattctcaaa ccctatccgg caagtgaaga acatgtatcg ctgctggccg
     1381 gcgaattgtc gaaacgccgg cgtttcgacg aagcgatcaa attgttgaag ctgaatcttg
     1441 atatgcatcc cggctcaccg gccttgtatg acagtctggc caaggcttat cggaagtccg
     1501 gcgaccagaa gatggcggac ctgtatgccg gaaaggcaca ggcggcggca gcgctccagc
     1561 cgggcgacaa accaaatgaa taggttctga cctgcagcat ggctccacct ccgggtggat
     1621 tttttttgtg ctataccgaa cgtttgccac tcatcggata cttttacctt cgtcgtccat
     1681 gcgtggccag ttgctttttt tcttcctact gctgatcgga tatcgaatgt cggcgcaaac
     1741 gacatttcca gatacaattc gttggtcggc ggatttttcg tcacagcagg ctgcagctgg
     1801 ttggatgaat ctggaccaac gacaagcttt gccgaacggg gatattgttt tggtgacgga
     1861 cagtacccat ccatacggtg ccggctattc ctggacctgg cccgatagct tgcctagccg
     1921 ctccgtcctg ttgcattgtg aagccaaact gcggtcttcc accctcaatc ccggtatcca
     1981 gtgggtgctt tcgatagaag agcagggaca aaccttattg tgggacggcc gggatctgag
     2041 caatcagttg gggaagcagg atgaattcat caatacaaac gtcgggttca agttgcctgc
     2101 taattttctg aggcgcggaa caaccatcaa agtctacctc taccagccgg accggcggtc
     2161 acgggtggaa acatccggtg taacgttcag tctctgccga tggggacttc tgtccttcct
     2221 gcctccgggc tcgccccaat ccgattcgag aagcgcggag caggaactct ttctgggaac
     2281 ggattacgcc gtacggatac agccggccgg tgggatcctg cgggtggatc gtcccgacgg
     2341 gcagcgactg atccgcaatg tgcttttata tacggaagca aagggcgatt ccatcacacc
     2401 gcggttcagt acgttggagt ttcaacgcga ttctgttgct cagggtgtgc accacctgct
     2461 gtttcgatca gccaatgaac ggttcaacac taccattacc ctggcaacag acgaaaaggc
     2521 ggcagcgata cgctggagcc tgaataccga atggcttcaa ccgactgaac tggtccgaca
     2581 ggctattgtg atgctggtgc agccggcagc cagccaggcg gtggatgtcg ttggaaggat
     2641 gctgcgggcc ggggatgacg aagaaagctg gttgggcagg ggtgccgtaa gttttggcga
     2701 tagcgttccg gagatgatcg tttcctatcc ggatcaactg tcttcgttgc aattggattg
     2761 ggcctccaac acgctttggt tgaatgccga ttggtcaatg gatcatccct tgctgcactg
     2821 gccgaaggag aagaagagcc gcaatcatcg cttggatagg agctgcagtg tgtataaagt
     2881 gcatgaacgc ctggaagccc gatggcaaac gtatttcaga atgccatcct ttcttcctgc
     2941 gctgcaagac cgtccgtttg gctacgatgc gacctttatc tggacggagc atgccgatta
     3001 ctcggatttg aaattgcaac gggcggtgat gtttggtcat gattccatct cggacccgtc
     3061 aacggcgaaa aaagggtttg tggcacgcgg cattccgatg accaagagtg tgttttacaa
     3121 caatccggat cgcgtcacga accgaaagcg tcatgcggcg ttcccgggta caatcgcgac
     3181 gatgagggaa acgccgggat ttgaagcatt gatgcagaca ctcgaggagt cgggtagtga
     3241 gatcgctttg catacccccg aacaattcac cagcaaccga aaacaattgg atgtcgcgtt
     3301 gcagtatatg cgatcccgat tcaataccac aacctggatc gaccatggtt acgacaatgc
     3361 tccgactagc aatcgggaag atttggtttg cgacggtctc gactcgctct cggacggcta
     3421 cgccttggat acatggttga ggtacgggat cacctcggtg tggaattgtt tttacgagga
     3481 tacgaatgtc tttctgccat actcgttcga ttcacgcctg gatgccccgt actccggcat
     3541 ttccggacga tttcctgcac cggtatggtg gacgcatccg acaagaagta aaagcatatt
     3601 gcattggcgt acgaatacta cactggatcc gccggatgca ggcatgtggt cctattacct
     3661 gggtgaacag cgcctggacg acttggtcag gcgtcgggga acttacatcg cgcattgtta
     3721 tcccgcgcgg gtggattcaa cgaatggttt ctatcgtctg aatggcggcc attgggagat
     3781 agagccggct ttcgatgacg cgctcgcctt gcaagcgcgt taccgggaag agcacaaact
     3841 gtggttgccg gtagtccggg aatatctgag ttatcagcaa aggatcagag aagtgaagat
     3901 ggttccaacc gaagatggct ggcgttattc caactcaggg caccaacccg tgcagggttt
     3961 tacactttat tcggaacacc tgctggatgc gggaagtaaa gcgatgcaga ttcggtcttc
     4021 cggctctggt cactactata gcttcaatct ggatccgggt gaaacagttt ttatcaagtt
     4081 gaagtaagaa tcaggcaatg gccgggaaaa aggtaacggg gaaaggcaag agtgggtggt
     4141 gggctcgtct gaggaaatgg acgattcgta tcgttgcggg atttttcatt ttaagcatcc
     4201 tgtccgtgat cctgttccgc ttcgttccgg ttccggttac tccgttgatg ctgatccgtt
     4261 gtgtcgagca gttgacggac ggtaggtcgt tacggatgag caaagattgg acgtccctcg
     4321 acaagatgag ccccgcatta ccacttgcag tcattgccgc ggaggatcag cgtttcgagg
     4381 agcatttcgg tttcgatttt gatgccatcc agaaagccca gcgttacaac gagcggcaca
     4441 aagggaaacg actgaagggg gcgagtacga tctcccagca gaccgctaag aacttgttcc
     4501 tttggccatc ccgatcctat gtacggaaag ggttcgaagt gtatttcact tttctgatcg
     4561 aagttttctg gagtaaggaa aggatcatgg aagtctatct caatattgtg gaaatggggg
     4621 agggcatcta tggcgccgaa gtggctgcga aggagtactt ccacaaaccg gccgcgcgac
     4681 tcaccaaccg cgaagcggcg ctcctggccg cagtcctgcc caatccccgg aaatggtcac
     4741 ctgcaagacc gacagcctac atacagcgta aggcgtccag gatcgtccgg ttcatgggta
     4801 acctggaact ggaggatttt tagccgggct ttaggcatgc tgttcaaaag aatgtaaatc
     4861 agtctttctt acccgcagga gtcggtataa atttgccctg ccttggagac cgctcaattt
     4921 tccaacgacc gcctgaagta cttcttgttc ttcctggccg ttctgctaag tcgcctgcca
     4981 tttatctctg atggttatgg ccttgacggg gattcatggg ccgtcgcgtt gacggcaagg
     5041 aacatccacg atacgggtag ctacgaagcg tcccggcttc cgggttatcc ggtgcaggaa
     5101 ctgctgagcg ctctgctgat caacggtggt gcgactgcgc tgaacttgtt ggtggcgatg
     5161 gtcagtgcgg cgggcatact ctttttcgct ctttcgctta aagttcttcg gtttcgtcac
     5221 cctttgctgg cggctgcagc cttagcggct gtaccggtaa tttacataca cagtacgaca
     5281 accatcgatt acaccatcgc gttgggtttt ctgctggcat ctttttattt cgtcgtttcg
     5341 gaaaaaccag tagtggccgg tattctcacc ggattggcca ttggcacgcg tgtcacctca
     5401 ggtgctatgc tgctgcccta tatcattatc ctgattggca atggtggtct gttcgtcaat
     5461 cttcgacgca tcctgttgta tgcagtgccg gcattgatag tcggtttgct gtgttttgct
     5521 cccgtcatcc agcgatacgg atgggatttc ctcacgtatt atcccgtacc ctatccctcg
     5581 atcgcgaaga tcctctacaa gttcacgttt gaagtgtggg gcgtggtggg actattcgcc
     5641 atttttcttg gcgtggtcat gttgttcctc ccgaacagga tcacgtccaa gcgctacttg
     5701 tttccccgct ctgtcaatga acgctatgtg atcgcctggc tgatcgcgat cgacctctat
     5761 gtcatcgcct ttttgcgctt gccgatggag tcaggatacc tggcgccggt tgtgccgttc
     5821 gtcattctga tcctcgggaa gtatctttat gaaccagctt tcaagttgtg ttgttacctg
     5881 ctgatcctgt cgcccttcgt aggttcgctt ggcccggtcg atcgactgga tgcggcaaag
     5941 ccatcgtcgg tcagtttccg ttttcgggcc gggggtgaga cgctggtgtt ggatgctttg
     6001 cgaggacctg tagttgccta caaatcgcgt cgtcaggagg gtatgaattt cacccgtgag
     6061 atcctgaact cggtggatag catcagccgg cctgcggtct tggtcagcgg tcgttggtac
     6121 aatcagttgg tcgtacagga aggagatacg acttccggta atctggtttt tgcgtcttac
     6181 ctcgacgaac ccatgctgtt gtattacatt ggtaaaaact actcggtgta ttatttgccg
     6241 aagcaggatt attacaacca gatcatgcgc aacgtcgatc tgaatctttt tggtgcacaa
     6301 gcctatctcc gggaagaaaa gtactgagga gggaaagggg attcaaatcc cgaagccgaa
     6361 accgatccgg atgatcggat tttgataggg cgaattggtt gattcgttga ggttgtacag
     6421 cagcatcagg ttgagggaag agttgtttcc cagcatctga cgataaccgc cgccgacgaa
     6481 gacggaggtg acattggttc tgcgaagtcg gtagggattc aagggatccg gtgcatcgag
     6541 attgaggatc tcgtattcgg tataggcgaa caggttttcc agaatgttgt agcgggaaaa
     6601 tacgcttccg ccgtacatgt tggtggaata ctcgaatggg gctgaacggt agcggtaata
     6661 gatatacttc ccgcctacgc cggcggcgag acggtcggtc agccggtaac cgacgatcgg
     6721 ggctatttcg atgatcgttt gtgtgccgaa ctgaagtcca aaatttccgc cgaagaatac
     6781 ccggtctatg aagcgatcac cttccgacgg ggccggtggt tgtagcatgg gtgattgtgc
     6841 ggaaaggaag ctggcgcaga ggcagcagga gaggagaagc aggaccctac ggatcatctt
     6901 gctaaattac gccaactttc gtgaaagcac ctagctggtt cgaaagctta cagattcagc
     6961 aacaactatg gcaacactgg tcattacggg cggatcgtcc ggtatcggat ttgaagcaac
     7021 tttgtggagc gcccgggagg gtcacaccgt catggtggtt tccaggaatc gcaccaggct
     7081 gggactgttg gaagccgaag ccgaaaagcg cggtgttcga gcgcgcgtgc acctgctgcc
     7141 ggcggatatt tcaaaacagc aggaagtgga agccattggt agggaaatac gctcgcgttc
     7201 cgccaggatc gatgcgttga tcaacaatgc gggtcgattg atcaacaaac cgttcggaga
     7261 attgcgggcc gaagactggg aagaagtcta cgcgaccaat gttttcggaa ccgtttccat
     7321 gagtcgggta ttgttgcccg gcttgatccg tagcagggaa gctttagcga ggggagaagc
     7381 ccctggtcat atcgtaaaca tctcgtcgat gggaggcgtg cagggaagtg ccaagtttgc
     7441 gggactctcc gcatacagtt cgagtaaggc tgctatcatc ggtgtgacgg aatgtttggc
     7501 ggaggagttg aagccaagag gtgtgcatgt aaatgcattg gcgttaggct cggtgcagac
     7561 ggaaatgttt tccgcggctt ttcccgggat agacgcttcg accactcctg agcgaatggg
     7621 ggaatatgta gcccgtttcg cgctggaggg gatgcactta ttcaacggaa aaacaatccc
     7681 ggtatcggca tccaccccat gaggcggaag accattgtgc ccggtaggcg gtaagcggtt
     7741 ggcagtgggc agtaggcagt aggcagtatc cggacaataa tcctaatccc gaataaccat
     7801 aaccaatcac caataaccag ttactaatta ccagttacca gttacaaatt cccaatctcc
     7861 catccccgtt ttccgctcca ttcctcgccc ccccacttgt tcataattgt tttcgctccc
     7921 ttgtttgcta attcaaaaac tcagagtact tttgtcctcc cattcacaat cgttctttgt
     7981 tgatatcgcg ccaccctggg aagtggcaaa atctgctgaa aagtggcttt tagattaaaa
     8041 gagttgagaa atcaactttt tttagctggt tttcataaga ttaagcgatt ttcaaaaaaa
     8101 atcttgcaaa aaggttgttt tgtacaagag tctttgtact tttgcagccc caatcaagaa
     8161 acgcgacgat ctgcaacggc agatttcgac atacgcgaac gttctttgag aaattgataa
     8221 ggaaacaaca ggctctattg ccctcaattc atattgagag taaatagcta ttgcgaatcc
     8281 caaaagtcaa atcaacttta ctatggagag tttgatcctg gctcaggatt aacgctagcg
     8341 gcaggcctaa tacatgcaag t
//
LOCUS       DAOTON010000015         8349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_7665_length_8349_cov_3.625030, whole genome shotgun sequence.
ACCESSION   DAOTON010000015 DAOTON010000000
VERSION     DAOTON010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7665_length_8349_cov_3.625030"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="P5292_00590"
     CDS             complement(<1..881)
                     /locus_tag="P5292_00590"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRS37650.1"
                     /translation="MKHFLRLLLLFPLLTGFGLRAQTFNANLAAMLQDSLDYYVNAIP
                     NIKGMSAGVYLPGQGYWQGQSGDSYAGQPITADMEFGIASNTKLFVSATMLKLAEDGL
                     LSLDDSVSAWLPAHPYIDSAITIRQLLNHTSGVSDPIFASPWMDTINANPTRVFTPQE
                     VLSWLGPPVFAPGASWSYSNVNYILAGMIAETASGFSIAQLIRDSLLTPLGLDSTFYD
                     VAEPERGTLAHRWWNSVDYHDTARTGLNSAGGCAGAIFSTAGEMCQWYHALFGGDVLQ
                     PASMTELTRFVPTTSSV"
     gene            1002..1892
                     /locus_tag="P5292_00595"
     CDS             1002..1892
                     /locus_tag="P5292_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS37651.1"
                     /translation="METAVPLTIVIPAYNEAENLKIVLPDLLELCEQHNWKIMITNDG
                     SKDDTRNVLQSFATNPRLSVVHHKLNKGYGAAIKSSIARCDTEYVITVDADGQHRKED
                     IVKLFDTIVAQDADMVVGSRKGTKAASRMRAVGKSIIRFTAKLLLTVPVHDINSGMKI
                     YRTELAQKYLHLTPNQMPFSDIITLIFISNRHLVLEVPIKVNDRLKGESTIGIQTAFE
                     TVIEILNIIVLFNPMKIFLPVSIITFLASCAWGIPIMLKGQGVSVGALLGFMSALIFF
                     FLGLIAEQLTLIRKNQKPND"
     gene            2041..4209
                     /locus_tag="P5292_00600"
     CDS             2041..4209
                     /locus_tag="P5292_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS37652.1"
                     /translation="MNKGMQKKKAVHKPNPSKTHAAPAEAGGSSIFSALQLLLIAFLG
                     ILIYSNSFDCSFHLDDRNSILENSAIRDLSNVGAIWKYSPTRFIPYLTLAFNYQLNGL
                     DVQGYHAVNLAIHIINAFLVWWLTALLFRTPNGKQSPLANRQRTIAFIVALLFVSHPL
                     ATQSVTYIVQRLASLATLFYLLSLCLYVKARLSETNARQKYGLFAGAFLAALLGLLSK
                     ENAYTLPFAIVLAELFFLRTEKPSAYLKDVRLLLLVAGIALAVFALYRGFLSTSGSGI
                     GSGGNAGNTSSVFSPIPPSGGTPYTVTPTNYLMTQFRVIVNYLQLLAFPTGLNLDHDI
                     RPSEQLFDAGTLLSLVFLLALLALAIYVFPKNRFISFGIFWFFLTLAVESSIIPISDL
                     MFEHRTYLPSFGFFLLITAGIFQVLYTKSGALTYALFTFIIGLNSLLTFQRNKVWKDE
                     LSLWNDVVSKSPEKARPYLNRGVAYWTAGQTAKAVSDYRKAISLNPEHYASAYFNLAV
                     AEAAMGQADQAIEHYTKAIAISPDYAIAYDGRGVVYGNRKEWDKALQDFSEAIRVSPG
                     FAKAYYNRGSLYSARSQWKEAISDYTKAVELDPKYTDAYCNRGVAYANIGEWDLSISD
                     CTASIRIDPRYVKAYFNRGTTFLNRGNPGAAAADFSSVLQYTPGNVLATYNRGLAYLQ
                     LKQWDNAIKDFTRTLQLDPGNQNAYTNREFAKSQLAVGGK"
     gene            complement(4756..4992)
                     /locus_tag="P5292_00605"
     CDS             complement(4756..4992)
                     /locus_tag="P5292_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37653.1"
                     /translation="MSSAELNSIKLSLISWINQLSDKELITFLEGLRTSSSKNDWWSA
                     LSVEQKKQVLAGLKDAEDGKVSDSGKFWKTLKNA"
     gene            complement(5275..6981)
                     /locus_tag="P5292_00610"
     CDS             complement(5275..6981)
                     /locus_tag="P5292_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37654.1"
                     /translation="MKFPINQFEQHIDGTILKRGLGYFKQGLVAEPEELRAGVYEAVV
                     SGTEDYRVRITIKDGMVTDHVCSCPYDLGPVCKHVVALLCWLQKDQLGIRETGATKRS
                     SRGTAKPKPAKPSIADRVNEILGRTPVEQLHQFIREQADQEPSFRNAFLAALGHTTGG
                     DSKAQYKQQLKALLRSAKGRDRFIAWNKTVIVGKGVYPMLRAAEKQLEQQDYMPVFHA
                     CTAVMEELVPALQYSDDSNGDLGGGITMAYDLLEKLAREPLPADLRKTLLDYCLGIFT
                     KGTFEGWDWHLGMLQVAALIVDEEKDADRIGQLIEKTKLSEYEQQHVQLIRLELIRRI
                     QGDQAAEKFLEKHLAHDNLRRKAIELAFGRRDYAKALRFAEDGVRRDSKGDADVATQW
                     HDWLLRIAQAQADREQIIKHARLLFLHDYRHEQDYYQVLKDTVPAGKWKSFVQALIRT
                     VVSGNRWTDYGLAEKIFIREEMWDKLLELLSIDPDLNRIERYESYLAKYFAPDLVRLY
                     AATIPEYIRLNSGRNHYQIAVRYMRRMKKLGGSEAVHALIVRLNQEFSKRKSLLEELE
                     RV"
     gene            complement(7496..7771)
                     /locus_tag="P5292_00615"
     CDS             complement(7496..7771)
                     /locus_tag="P5292_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37655.1"
                     /translation="MLLIVLRSDHASLKENYARLKAFSPEQLVNAYNQSYHVGIIGSK
                     AQGLYLLAMHKVFTDTFGRSPIELIEDCIIRFSKPIVLINGQWKHIE"
     regulatory      complement(7834..7927)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
BASE COUNT         1940 a         2393 c         2110 g         1906 t
ORIGIN      
        1 ataaccgaag aggttgtggg aacgaagcgc gtcaactccg tcatcgacgc cggttgcagc
       61 acgtctccgc cgaacagggc gtggtaccac tggcacatct ccccggcggt ggagaagatc
      121 gcgcccgcgc aaccacccgc gctgttgagt ccggtacggg cggtatcgtg atagtcgacg
      181 ctgttccacc agcggtgcgc gagcgttccg cgctcgggtt cggcgacatc gtagaaggtg
      241 ctgtcgaggc cgagtggcgt cagcaggctg tcgcggatca gttgcgcgat ggagaaaccg
      301 ctcgcggttt ccgcgatcat gccggcgagg atgtagttga cattcgaata gctccagctt
      361 gcgccgggcg cgaacaccgg cggtcccagc cagctcagca cttcctgcgg tgtgaacacc
      421 cgggtcgggt tggcgttgat cgtatccatc cagggtgagg cgaagatcgg gtcggacacc
      481 ccgctggtgt gattgagcaa ctgccggatg gtaatcgcgg agtcgatgta cggatgcgcg
      541 ggcagccagg ccgataccga atcgtcgagc gagagcagtc cgtcttcggc caacttcaac
      601 atcgtggcgg agacgaacag tttcgtattg ctcgcgatgc cgaattccat atcggccgtg
      661 atcggctgtc ccgcgtacga atcaccgctt tgtccctgcc aataaccctg cccgggcagg
      721 taaacaccag ccgacatccc tttgatgttc gggatcgcgt tcacgtagta atcgagtgaa
      781 tcctgcaaca tggcggccag gttcgcattg aacgtctgtg cccgaagacc gaagccggtc
      841 agcaacggaa acaacagcag caatcgaaga aaatgtttca tggcagcctc gtttccccga
      901 agataatcat tttgaaacct acacgcagtc tactcccgga agcaccgtaa aaaacctaca
      961 ttagtgcaat caagcctacc gtcaattccc acataccctg aatggagacc gcagtaccgc
     1021 tgaccatcgt catccccgct tacaacgaag ccgagaacct gaagatcgtg ctacccgatc
     1081 tgctcgaact ctgcgagcag cacaattgga agatcatgat caccaatgac gggtcgaaag
     1141 acgatacgcg aaatgtcctc cagtcgtttg ccacgaatcc ccgcctgtcg gtggtgcacc
     1201 acaagctgaa caaagggtac ggtgcggcga tcaagagctc gatcgcgcgc tgtgatacgg
     1261 aatatgtcat caccgtcgat gccgacggac aacaccggaa agaggacatt gtcaaactct
     1321 tcgacaccat tgtcgcgcag gacgcggaca tggtcgtcgg ctcgcggaaa ggaaccaaag
     1381 cggcttcccg gatgcgggca gtcgggaaat ccatcatccg gttcactgcc aaactcctgc
     1441 tgaccgtgcc ggtacacgac atcaactcgg gtatgaagat ctaccgtacc gagttggcgc
     1501 aaaagtacct gcacctgacg ccgaaccaga tgcccttcag cgacatcatc acgctcatct
     1561 tcatcagcaa ccgacacctg gtgctggagg ttcccatcaa agtgaacgac cggctgaagg
     1621 gagaaagcac gatcggcatc cagaccgcgt tcgagacggt gatcgagatc ctgaacatca
     1681 tcgtgctgtt caatccgatg aagatcttcc tgccggtctc gatcatcacc ttcctcgctt
     1741 cctgcgcctg ggggatcccg atcatgctga aaggccaggg cgtcagcgtc ggcgccctgc
     1801 tggggttcat gtcggccctg atctttttct tcctcggcct cattgccgaa caattgacgc
     1861 tgatccgcaa gaaccaaaag ccgaacgatt gatccggccc ggaggcccgg ctgtgagaat
     1921 ttgtcacccg ccccgtgctg acaaatgttt ctggcaggct atcgggcccg gaatacttcg
     1981 cctcggattt gcattatctt tatccgattc tccacagcgc cacacgaaca agtaccccgt
     2041 atgaacaaag gaatgcaaaa gaaaaaagcg gtccataaac cgaatccgtc gaagacccat
     2101 gcagcaccgg cggaagccgg cggttcgtcg atcttctccg ccctgcaact gctcctcatc
     2161 gcctttctcg ggatactgat ctattccaac tccttcgatt gttcctttca cctcgacgac
     2221 cggaacagca tcctcgagaa cagcgcgatc cgcgacctct ccaacgtcgg agcgatttgg
     2281 aaatactccc cgacccgctt cattccttat ctgacccttg ccttcaacta ccagctcaac
     2341 ggactggatg tccagggcta tcacgcggtc aacctcgcga tccacatcat caacgcgttc
     2401 ctcgtgtggt ggctcaccgc gctcctgttc aggacaccga acggaaaaca atctcccctt
     2461 gcgaaccgcc agcggacgat cgcctttatc gtcgcgctgc tgtttgtctc acacccgctg
     2521 gccacgcaat cggtgaccta catcgttcag cgactggctt cgctggccac cctgttctat
     2581 ctcctgagcc tgtgtctgta cgtgaaagcg cgcttgagtg aaacgaacgc gcgacagaaa
     2641 tacggactct ttgccggcgc gttcctggct gcactacttg gactcctctc gaaagaaaac
     2701 gcgtacacgc tgccgttcgc gatcgtcctg gcggagctgt ttttcctgcg gacggaaaag
     2761 ccgtccgcct atctgaaaga cgttcgcctc ctactcctgg tagccggcat cgcgctggcg
     2821 gtcttcgcgc tgtaccgtgg gttcctgtcc acctccggtt cgggcatcgg ttcgggtggt
     2881 aatgccggca ataccagcag cgtcttttcg cccatcccgc catccggtgg cactccttac
     2941 accgtcaccc cgaccaatta cctgatgacg cagttccggg tcatcgtcaa ctacctgcaa
     3001 ctgctggctt tcccgaccgg gctcaacctc gaccacgaca tccggccttc ggagcagttg
     3061 ttcgatgccg gcaccctgct ctcactggtg ttcctgctgg cgctgctggc cctggcgatt
     3121 tatgtctttc cgaaaaaccg gttcatctcc tttggcatct tctggttctt tctcaccctg
     3181 gcggtggagt cgagcatcat cccgatttcg gacttgatgt tcgagcaccg gacctacctc
     3241 ccgtcattcg gtttcttcct gctgatcacc gcgggaattt tccaggtgct ttatacgaag
     3301 tccggcgcgt tgacctatgc gctctttacg ttcatcatcg gcctgaactc cctgctcacg
     3361 ttccaacgga acaaggtctg gaaagacgag ctgagcctgt ggaacgacgt agtctcgaag
     3421 tcgcccgaga aggcccggcc ttacctgaat cgcggagtcg cgtactggac cgcgggacag
     3481 accgcgaaag cggtatcgga ttatcgaaag gctatctcgt tgaatcccga acattatgcc
     3541 tccgcctatt tcaacctcgc cgtggcggaa gcggcgatgg gacaagcgga ccaggcgatc
     3601 gaacattata cgaaggccat tgcgatcagt ccggattacg cgatcgccta tgacgggcgc
     3661 ggagtcgtgt acggcaaccg gaaggaatgg gacaaagcct tacaagattt ctccgaggcg
     3721 attcgtgtct ctcccggctt cgccaaagcc tattacaacc ggggcagttt gtattcggcc
     3781 cgttcgcagt ggaaagaagc gatcagcgat tacaccaagg cggtcgagct ggacccgaag
     3841 tacaccgatg cttattgtaa tcgcggagta gcctatgcca acatcggcga gtgggacctg
     3901 tcgatctccg actgcacggc gagcatccgc atcgacccgc gctatgtgaa agcctacttc
     3961 aaccggggca ccaccttcct gaaccggggc aatcccggcg ccgcggcggc ggacttcagc
     4021 agtgtcttgc agtacacccc cggaaacgtg ctggccacct acaaccgcgg actggcctat
     4081 ctacagctca agcaatggga taacgcgatc aaggatttca cgcgcaccct gcaactcgat
     4141 ccaggcaacc aaaacgccta caccaatcgc gaattcgcga agagtcagtt ggcggtgggc
     4201 gggaaatagt gatgtcttcc aaaaaaatga agccttgcgg ctgcagtgcg tggggcggaa
     4261 tgcgcgcggg agcttaaacc actttactca acgcttcgtg cgacactaaa cattccgtca
     4321 agccacggca ttgggcggaa tgcgcgctgg agcgtaaacc actttactca acgcttcgtg
     4381 tggcgcttaa cattctgcca agccacggta gtggggacat tcagcaaaat gcgggataag
     4441 gcggtttatc ttccggtctt taatcggcta ctggtgtcgc agcgattgtc cagcaaggcc
     4501 agcacctcta ttcttccccg attgatccga taaaatgctg aaagctcctt gctgagaact
     4561 gctcttctta cacttctctt ataggtagat cgtggataca gttcaggaaa tgcgcaaacc
     4621 gcgacttcaa atgattcaag cagtatataa aacttttgaa cttccttccg gctaaatctc
     4681 gtcatcaggt agcgttttat ttgaatcgca tgttgtaagg aattttccgt ccatacgatc
     4741 gatacggcct gcgacttagg cattcttcaa cgttttccag aactttccgg aatcggacac
     4801 cttgccgtcc tcagcatcct ttaagccggc caacacttgt tttttctgtt cgacagacaa
     4861 ggctgaccac cagtcattct ttgaactgga cgtccgcaag ccttccagaa aagtaatcag
     4921 ttccttatcg gacagctggt tgatccagga aatcaggcta agcttgatgc tattcaattc
     4981 cgctgatgac atgatatcaa aattgtatta aacaaatata cgaacttcta tcatgaatcg
     5041 ttggagcaac cttgacaatg cgaaatgtta acgcgatcgc caagttctca atcgtacatt
     5101 agcaactgcg cgcattacgc tccaaacgca acatccaacc actaccgatg attggcggga
     5161 tgttaagcgc cttcgctatg ctgaaaattg cagtacaaca gcagcgcgca ttccgcccaa
     5221 aacgcaccgg cctgccaata aaaaatgaag aaacacaccg ccaaaccacg gagttcaaac
     5281 ccgctccagc tcttccaaca gactcttacg tttgctgaat tcctgattca aacgaacaat
     5341 gagtgcatgc acggcctcgc tgccgcccag cttcttcatg cgtcgcatat agcggacggc
     5401 aatctgatag tgattccttc cggagttcag gcggatatac tccgggatgg tcgcagcgta
     5461 caatcggacc aggtcgggcg cgaagtattt ggccagatag gattcgtagc gttcaattct
     5521 attaaggtcg ggatcgatcg aaagcagttc caacaattta tcccacatct cttcccgtat
     5581 gaagatcttc tcggccagtc cgtaatcggt ccagcggtta cccgatacca cggtccggat
     5641 gagtgcttgc acgaatgact tccatttacc tgccggaacc gtatccttca gaacctggta
     5701 gtagtcctgt tcgtgcctgt aatcatgcag gaaaagcaat cgtgcatgtt taatgatctg
     5761 ctcacggtct gcttgtgcct gagcaatcct cagcagccaa tcgtgccatt gggtcgcaac
     5821 atcagcgtca cccttgctat ctctgcgcac accgtcttcg gcaaagcgca acgcctttgc
     5881 ataatcccgg cggccgaacg ccagctcgat ggcttttcgc ctgaggttgt catgggccag
     5941 gtgtttttcc aaaaacttct ctgcagcctg atccccctgg attcttcgaa tgagttccag
     6001 gcgaatcaac tggacgtgtt gctgttcgta ttccgagagc ttcgtcttct ctatcaattg
     6061 cccaatccga tcggcgtctt tttcttcatc tacgatcaac gcggccacct gcaacatgcc
     6121 gaggtgccaa tcccagcctt cgaaggtccc ttttgtgaag atgccgaggc aataatcaag
     6181 cagcgtcttt ctcaaatcgg ccggtaaagg ttcccgggcg agtttttcca acaagtcata
     6241 cgccatggtg atgcctccgc ccagatcccc gttgctatcg tcggaatatt ggagcgccgg
     6301 caccagttct tccatgactg ccgtgcaggc atgaaagaca ggcatgtagt cctgctgctc
     6361 cagctgtttt tcagcggccc tcagcatcgg atagacccct ttgccgacga tgaccgtctt
     6421 gttccaggcg atgaagcggt cgcggccttt cgcgctccgg aggagcgctt tcagttgttg
     6481 tttgtactga gctttcgaat caccccctgt cgtgtgtccc aacgcagcca ggaaagcatt
     6541 ccgaaaggaa ggttcctggt ctgcctgctc ccggatgaat tgatgcaact gttccaccgg
     6601 cgtccggccg aggatctcgt tgacgcggtc ggctatggac ggtttggcgg gtttgggttt
     6661 tgccgtaccg cgactgcttc gcttcgttgc tcctgtttcc cggataccca actggtcctt
     6721 ttgcagccag cacagcaacg cgaccacatg cttgcagacc ggacccagat catatggaca
     6781 ggaacagaca tgatcggtca ccataccgtc tttgatcgtt atccggacgc ggtagtcttc
     6841 cgtccccgaa acaacggcct catagacccc ggccctcagc tcctctggct ccgcaaccag
     6901 cccctgctta aagtagccaa ggccacgctt gaggatcgtt ccgtcgatat gttgctcgaa
     6961 ctggttgatc gggaatttca tcgattgaaa ttttgctcgc ggacaacgag caattatact
     7021 acttcagaac actaaatcca tcatgaacct tgcaacacaa acgcaccggc cctacaaagt
     7081 aaatgaagcc atgcgtgaaa taaaacaatc taccctctta cttaacttct ttcttgccaa
     7141 gaccaatgca ttgtcgagta tcccaccttg cggacaagta aatgaaaacc atgctgtacc
     7201 tggcgtccct aagttcgatc agcaggtccg gagagtttaa agattacttc gatcgaaggg
     7261 tgaaagaagg aaagaacaaa atgctagtga tcaatgccat ccgaaacaag cttatacacc
     7321 gcatattcgc ctgtgttaag gaacagagaa attatgtgaa gaaaggcttg caagtggcat
     7381 agaaatcgcc caaagagagc ggggaaagcc cagctactgg gtggcagacg ttcgccgaaa
     7441 agggttacgg aagaatgcat cggggaactc aaggaaggcc gaagcaattc aaaccctact
     7501 cgatatgttt ccattggcca ttgattagca ctatcggttt gctgaaccgg atgatacagt
     7561 cctcgatcaa ttcgatcggc gaccgcccaa aggtgtccgt gaagacctta tgcatggcaa
     7621 ggagatagag tccctgtgcc ttgcttccga tgatacctac atggtaactc tggttatagg
     7681 cattgaccaa ctgctcgggc gaaaacgctt tgagacgagc gtaattctcc tttagactgg
     7741 catgatcaga ccggaggaca ataagtaaca tttccatttt tacaaatgat ttgttgttta
     7801 cacattcatt tgcaatctcg aaaaatggag ggcgctaagc gtttattttg gtttgaccca
     7861 catcatttta ccaccgtggt gtgtcgccac tcggttggtg ctcacttaag agctcgaaat
     7921 gcgacataaa attacagcga aatttagttt taacaagggg gtcaagtgaa ataatctaag
     7981 cctgaatatg cgggaagtct tcatcatggt agttcaatat aatcaagagg gagctggcgg
     8041 tagatgtgga aactagttca agatcactga ttacgctgaa ttctctaatt tgatggagcg
     8101 tttacacatc ctcgtctaat tcacacaaca tccacgcacc ggctccgtcg gaaatcagga
     8161 gggtcactgg cgcaccaggc gatggtgctt cacgcgttcg cccgctttta ttggttgaat
     8221 gaaagacagt gattggcaca tcttcgcccg tgccatttgc ttaccagata cacaaacagg
     8281 acgcggccta ctttctcacg atgacggcgg tggaatgggt agaggtgctt tcgcggcggg
     8341 aagcgaagc
//
LOCUS       DAOTON010000016         8260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_7758_length_8260_cov_3.478123, whole genome shotgun sequence.
ACCESSION   DAOTON010000016 DAOTON010000000
VERSION     DAOTON010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7758_length_8260_cov_3.478123"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="P5292_00620"
     CDS             complement(<1..804)
                     /locus_tag="P5292_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRS37656.1"
                     /translation="MKTYKLYIDGKFPRTESGRYDIFTDASGKAIANICRASRKDFRD
                     AVVAARKAQPGWAARSAYNKGQILYRIAEMLEGRRAQFIEELGTEGLSPAAAGAEVSA
                     AVDRLIYYAGWSDKYQQVFSAVNPVESSHFNFSMPEPTGVVSMIAPAAHGLLGLVSVI
                     APAIVGGNTVIVLSSEAHPLTAITFSEVLHTSDLPGGVVNILTGQVAELVGHFSNHMD
                     VNAVVYCGDDAGFRKTIQEHAALNVKRPFFYHGDWTAETAQGPYRIMDLQ"
     gene            complement(918..2396)
                     /locus_tag="P5292_00625"
     CDS             complement(918..2396)
                     /locus_tag="P5292_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRS37657.1"
                     /translation="MAKKKPIAGLKFNGGWDYAAAPESKDHIRLQKQYDLFINGKFVA
                     PKSGKYFDTINPATEAKLASVAEAGADDVDLAVKAARQAYEQVWSKLPARERGKYIFR
                     IARIMQERARELAVIESMDGGKPIRESRDIDVPLAAAHFFYYAGWADKLDYAMPGARA
                     GSLGVAGQIIPWNFPLLMAAWKIAPALAAGNTVVIKPAETTPLTALKLAEIIQEADLP
                     PGVVNIITGAGATGAAIVNHPGVDKVAFTGSTDVGKIIQRALAGTDKKYTLELGGKAA
                     NIIFEDAAIDQAVEGIINGIFFNQGHVCCAGSRLFVQESVADKVIRKLKDRMETLVVG
                     DPLDKNTDIGAINSKPQLEKIRQFLKIGVNEGGTLYQSSCAVPSKGYWCRPALFTDVS
                     QSSRIVQEEIFGPVLAVQTFRTIEEVIEKANNIPYGLSGGVWTDKGSRIFKITGQLRA
                     GVVWANTYNKFDPTSPFGGYKESGMGREGGLHGLHPYVKINA"
     gene            complement(2444..3364)
                     /gene="deoC"
                     /locus_tag="P5292_00630"
     CDS             complement(2444..3364)
                     /gene="deoC"
                     /locus_tag="P5292_00630"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRS37658.1"
                     /translation="MMREERRAAVFGAWDSPPVDQVGVEERISRLGKRSIKKESKLSA
                     LKIAMSMLDLTTLEARDTPGKVRQLCIKAMHPHDAVPGIPSVAAVCVYPNLVRVAKET
                     LQGSKVKVAAVATAFPSGMSTRKVKLEETRWAVDEGADEIDMVISRGHFLRGEYGFVF
                     DEIAAVKEACGKAHLKVILETGELSTLDNVRRASDLAIQAGADFIKTSTGKIQPAATL
                     PVTLVMLEAIRDHYYNTGVKIGMKPAGGIATSKLALQYLVMVRETLGQDWLNADLFRF
                     GASALANDLLMQILKEQTGVYQSIDYFSKD"
     gene            complement(3451..4059)
                     /locus_tag="P5292_00635"
     CDS             complement(3451..4059)
                     /locus_tag="P5292_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRS37659.1"
                     /translation="MRNACCIAGITLFLLSCNLSVSAQDFRAGIKAVLCASQVSGDRL
                     SGFDKAGFAAGMFVRRDLSEQVAFQLEIQFIQKGSRKPLNKDDNTYYRMRLHYAEVPV
                     LLRVKPGKKWTFEAGAAYGSMLFSQEDNELGEIQGTPPFRKYELSWILGMDYPLGEKV
                     LFNIRYNHSITPVRTFEGSYNYNYWDRGQYNAVLQGGFYFAF"
     gene            complement(4056..5012)
                     /locus_tag="P5292_00640"
     CDS             complement(4056..5012)
                     /locus_tag="P5292_00640"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HRS37660.1"
                     /translation="MKNYRFLLSASCGVLLLALISLSACDKIDAPYREQTAVGVTYLS
                     DDAVVINGDTLEFPADNTTPEKHVLIEDYTGILCGNCPYAGLKLNDTLKPLFGDRLVA
                     MAVHAGFFADPCPQGLACSGSQPAGALETDFRTAVGDAWDTKFGNSNAGNPNGLVDRI
                     GYPTNQQVKFPNQWRGLIENRLLESTPFRLRIQTEFVDASREVKVAVQSENVAALNAT
                     YKLQVVITEDSLVDWQVWYPPRTPEYDPSFLHRHVLRSSLNSEFGEKIYEGTVAAGDK
                     VLRGYSATLPAGQVAAHCYAIAFIYNADTYEVLQVAERALIE"
     gene            complement(5016..6644)
                     /locus_tag="P5292_00645"
     CDS             complement(5016..6644)
                     /locus_tag="P5292_00645"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HRS37661.1"
                     /translation="MKRILCLLGLFALAGASKAQGLPNLPIHGNVQLDAQYYFEDSLI
                     GAPDVPEQMLFNGFANFTYEKDNFSAGLRYESYQNPILGFDKRYKGSGIPYRFLRYKN
                     NELDITAGTFYEQFGSGILLRAYEERGLGYDNAIDGFRFMYNPAKGVYFKGVIGKQRD
                     FFNLGQGIVRGGDVEWVVNESCESRAAAKTQWIIGGNYVSKYQSDEDPTFRLPENVGS
                     GSGRVSFTNGGWSASAEYAYKANDPSASNGFIFKEGQALLLNASYTKKGFGFVLGAKR
                     IDNMSFRSDRYATVNSLLVNYLPALTKNHTYLMAAFYPYATQPNGEFGFQGELFYKLK
                     PGGLGGKYGTDINLNYSRAQSIEKKAITDPEQADLGYTSDFFALGKEVYFEDFNIELN
                     RKLSKEVRLVLTYLYQNYNRDVIEGRTGFGHIYSHIGIAEVFYRISPKKNFRTELQHM
                     YTEQNTKSWAVLLAEYTVAPHWFVAAFDQWNYGNDDSNERFHYITGQFGYTRGSNRVV
                     VGYGRQRPGILCVGGVCRNVPAANGFTLSVTSSF"
     gene            complement(6786..7316)
                     /locus_tag="P5292_00650"
     CDS             complement(6786..7316)
                     /locus_tag="P5292_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HRS37662.1"
                     /translation="MKKSLVVVLLFLTAFTGFAQDQPATVRPLPKVNVKTLSGETVNT
                     ETIANDGKPIIIDFWATWCKPCIEELTTIHDLYADWQKETGVKLIAVSMDDQRTMTRV
                     APFVNGRGWNYENYVDPNKDLARALNVNMPPHTFVLNGKGEIVWQHVGFAEGNELELY
                     EVVKKVAAGQPLDEKK"
     gene            7558..>8260
                     /locus_tag="P5292_00655"
     CDS             7558..>8260
                     /locus_tag="P5292_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRS37663.1"
                     /translation="MKKLLLSLVALFALGTATVNAQMAAGNIAPDFTGTDINGNTWNL
                     YSLLDQGKTVYIDVSATWCGPCWNYHNGHALEDLYAAHGPTGTTSQDVMVFFVEGDAS
                     TTLADLQGTGGNTQGDWVTGTPYPIIDDATIGDALQISYFPTIYIICPDRIIKEVGQL
                     TATALYNQRNSNCSFATSTDDAGITNSFTALNGTLATCDVVDLDYRLCNYGTTPLTSA
                     TIDLTVNGTVQQSYNW"
BASE COUNT         1872 a         2350 c         2117 g         1921 t
ORIGIN      
        1 ctgtaagtcc atgatccggt aagggccttg ggccgtttca gcggtccaat cgccatgata
       61 gaagaatggc cgcttcacat tgagggccgc atgctcctga atggtctttc tgaatccggc
      121 atcgtcgccg caatacacaa cagcgttcac gtccatgtga ttggaaaaat gccccacaag
      181 ctccgccact tgcccggtca ggatgttgac tacacctccc ggaaggtcgg aggtatgcaa
      241 cacttcggag aaggtgatcg ctgtcagcgg atgcgcttcg gaactcagca cgatcacggt
      301 attgccacct acgatcgcag gtgcgataac cgaaacaagt ccgagcagcc catgtgccgc
      361 gggggcgatc atgctgacaa caccggtcgg ttccggcatc gaaaaattga aatgggacga
      421 ttccaccgga ttcaccgcgc tgaaaacctg ctggtactta tcactccagc cggcataata
      481 gatcaaccgg tccaccgccg cggaaacttc cgcgccggca gcggcaggac tgagcccttc
      541 cgttcccaac tcctcgatga attgtgcacg ccggccttcc agcatctcgg cgatgcggta
      601 caagatctgt cctttattgt acgctgaacg cgccgcccat ccgggttgcg ccttgcgcgc
      661 ggcaacgacg gcgtcgcgaa aatccttccg ggaagctcga cagatgttgg cgatggcctt
      721 tcccgaggcg tccgtgaaaa tatcgtagcg tccggattct gtccgaggga atttcccgtc
      781 gatgtagagt ttataggttt tcatcacccc gatgcgtccc gctccggcag cctgcccgtt
      841 gccgacagcc tgctgaccag tcagggtgga agatttctcg tttgccattg tttcgattct
      901 taactactgt tagcggatca ggcgttgatc tttacataag gatgcaagcc gtgcaggcct
      961 ccttcacggc ccatgccgct ctctttgtaa ccgccgaatg gcgaggtggg atcgaattta
     1021 ttgtaggtat tcgcccacac caccccggca cgcaattgac cggtgatctt gaagatgcgt
     1081 gaacctttgt ctgtccaaac accaccactc aatccatacg gaatgttgtt ggctttctca
     1141 atgacttctt caatggttcg gaaagtctgc acagcgagca caggaccgaa gatctcttct
     1201 tgcacgattc gagatgactg cgacacatcg gtgaacaagg cagggcggca ccaatagccc
     1261 tttgacggaa ccgcgcagga agattgatac agggtgccgc cttcgttcac tccgatcttg
     1321 aggaactgac ggatcttttc cagctggggt ttggagttga tggcgcctat gtcggtattc
     1381 ttatccagcg gatcgccgac aaccagcgtc tccatgcgat ccttcagttt acggatcacc
     1441 ttgtcggcaa cggattcctg cacgaacagg cgcgacccgg cgcaacagac gtgaccctga
     1501 ttgaagaaga tcccgttgat gatcccttcc accgcctgat cgatcgcggc atcctcgaag
     1561 atgatattgg ccgctttgcc gcccaactcc agcgtatatt tcttatcggt cccggccaac
     1621 gcgcgctgga tgatcttacc gacatcggtc gaacccgtga aggccacttt atcgactccc
     1681 ggatgattga cgatggccgc gccggtagct ccggcaccgg tgataatatt gacaacgccg
     1741 gggggaaggt cagcctcctg tatgatttca gccagtttca gggcggttag cggcgtggtc
     1801 tctgccggct tgatcactac cgtatttcct gcggcaagcg caggcgcgat cttccaggcc
     1861 gccatcagga gcgggaagtt ccacggaatg atctgaccgg caactcccag cgagcccgca
     1921 cgagcgcccg gcatcgcgta atccagctta tccgcccagc cggcgtaata gaaaaagtgc
     1981 gcagccgcta atggaacatc gatatcgcgt gattcacgga tcggtttacc gccgtccata
     2041 ctctcaatca ccgccagttc ccgcgcacgc tcctgcatga tgcgcgcgat gcgaaagatg
     2101 tacttacccc gctcgcgcgc cgggagcttg ctccaaacct gctcgtaggc ctgacgcgcg
     2161 gccttcaccg cgagatctac gtcgtccgct cccgcttcag cgacggaagc caattttgct
     2221 tccgtggccg ggttgatcgt atcaaaatac ttcccgctct tcggggcaac gaattttccg
     2281 tttatgaaga gatcgtactg cttttgcagg cggatatgat ccttgctttc cggcgcggcg
     2341 gcataatccc atcctccgtt gaacttcaga cctgcgattg gttttttctt tgccatctgt
     2401 tgaatgcctg tgaacggttg cggccaggct gccgttcccc tgattagtct ttggaaaaat
     2461 aatcgatgct ctgatacacg ccagtctgct ctttcagtat ctgcatgagc agatcgttgg
     2521 ccagagcgct tgctccgaaa cggaacaggt cggcgttcaa ccaatcctgt ccaagcgttt
     2581 cacgcaccat gaccaggtat tgcaaggcca gcttggacgt ggcgattcca cctgccggct
     2641 tcatcccgat cttcactccc gtgttgtaat agtggtcgcg aatggcttcc agcatgacca
     2701 gtgtaaccgg caaggtggcg gccggctgga tcttacccgt actggtcttg ataaaatccg
     2761 ctccggcctg aatggcgaga tcactcgccc ggcgcacgtt gtccaaggtg ctcaactcgc
     2821 ccgtttccag gatcaccttc aggtgagcct ttccacaggc ttcctttacg gcagcgatct
     2881 catcgaatac aaaaccgtat tcaccacgga ggaaatgtcc gcgcgaaatg accatgtcga
     2941 tctcatccgc accttcgtcg actgcccaac gggtctcttc gagtttcact ttacgggtag
     3001 acattccact ggggaacgct gtggctaccg cggcaacttt gaccttcgag ccctgtagcg
     3061 tctctttcgc gactcgtacg aggttgggat agacacaaac agcggcaacg gagggtatgc
     3121 caggaaccgc atcgtgcgga tgcatcgcct tgatacacag ttgacggacc tttccaggcg
     3181 tatcccgggc ttccagggtc gtcaggtcaa gcatgctcat ggcaatcttg agtgccgaca
     3241 acttcgactc tttcttgata ctccgtttgc ccagccggga gatccgctct tccacgccaa
     3301 cctgatcgac gggcggactg tcccatgctc cgaatacggc agcacgacgt tcttctctca
     3361 tcatgaaaag ttattcctac gaaggtagga atttacggga aaaatgaagg aatgggaaag
     3421 acctctctgg ctattgcaaa agccgactga tcagaatgcg aaatagaagc cgccttgaag
     3481 aacagcattg tactggccgc ggtcccaata gttgtaattg taggatcctt caaatgtcct
     3541 gaccggcgtg atcgaatgat tgtaccggat attgaagagt accttttcgc ctaagggata
     3601 atccattccc agtatccagg acaattcgta cttacggaat gggggtgttc cctggatctc
     3661 cccgagttca ttgtcctcct gtgagaataa catcgatcca tacgccgctc cggcctcaaa
     3721 tgtccatttc ttgcccggtt tgacacgcaa caacaccggc acctcagcgt aatgcaaccg
     3781 catccggtag taggtattgt cgtccttgtt gagtggtttc cggcttcctt tctgaatgaa
     3841 ctggatttcc agttggaatg ccacctgttc actcagatcc cgtcgcacga acatgccggc
     3901 cgcgaatcct gctttatcga atcccgacag acgatcacct gaaacctggg aagcacatag
     3961 caccgccttg attcctgctc tgaaatcctg cgcgctaacg gacaggttac aggagagcag
     4021 gaacaacgtg atcccggcga tacaacatgc atttctcatt cgatgagggc gcgctcggct
     4081 acttgcagga cttcgtaggt atcggcatta taaataaagg cgatcgcata gcagtgagcg
     4141 gcgacctgcc ccgcaggcaa tgttgcgcta taaccgcgca gcactttgtc gccggcagcg
     4201 acagtccctt cgtagatctt ttcaccgaac tccgagttca gggaactgcg caagacgtga
     4261 cgatgcagaa aactgggatc gtattccggc gtacggggag gataccagac ctgccagtcg
     4321 acaagactat cctccgtgat gacaacctgc aatttatagg tggcattcag cgcggcgaca
     4381 ttctccgatt gcactgcaac tttcacttcg cgggaagcgt ccacgaattc cgtttggata
     4441 cgcaaccgga acggcgtgct ctccagcaat ctgttctcga tcaagccccg ccattggttc
     4501 ggaaacttca cctgctggtt ggtcggatac ccgatccggt caacgagtcc gttcgggtta
     4561 ccggcattgg aatttccaaa cttggtgtcc caggcatcgc caacggctgt acgaaaatca
     4621 gtctccaggg caccggcggg ttgacttccg gaacaggcca atccctgcgg acaaggatca
     4681 gcaaagaatc ccgcatgcac cgccatggcc accaggcgat cgccgaacaa cggtttgagg
     4741 gtgtcgttca gtttgagtcc ggcatacggg caattgccac acaggatgcc ggtgtaatct
     4801 tctatgagca catgcttttc aggagtggtg ttgtctgccg gaaactcaag ggtgtctccg
     4861 ttgatgacca ccgcatcatc gctcagatag gtcaccccga cagccgtttg ctcgcggtaa
     4921 ggggcgtcga ttttgtcgca tgcgctgaga ctgatcaggg ccaacaacag aactccacag
     4981 gaagcggata agaggaaacg atagttcttc atgcgtcaga agctactggt aacagataag
     5041 gtaaaaccat tggcggcggg cacattccgg cagacaccgc ccacgcacag gatacccggg
     5101 cgttgacgtc cgtacccgac taccacgcga ttggaaccgc gtgtataacc gaattgcccg
     5161 gtgatgtaat gaaagcgttc gttggaatcg tcgttgccgt aattccactg gtcaaatgcc
     5221 gccacaaacc aatgcggagc cacggtatat tccgctaaca gcacagccca gctcttcgta
     5281 ttctgttcgg tgtacatgtg ctggagctct gtccggaaat tcttcttcgg actgatccgg
     5341 taaaagactt cggcgatccc gatgtggctg taaatatggc cgaagccggt tcgcccttcg
     5401 atgacatcgc ggttgtaatt ttgatagagg taggtgagca ccagtcggac ttccttgcta
     5461 agcttccggt tcaattcgat gttgaagtct tcaaaataga cttccttacc caacgcaaaa
     5521 aaatccgatg tatagccaag atccgcctgc tcaggatcgg tgatcgcctt cttttcgatc
     5581 gattgagcgc gggaataatt aaggttgatg tccgtgccgt acttaccccc aaggccaccg
     5641 ggcttcagct tgtagaacaa ttctccctgg aaaccaaatt caccgttcgg ctgggtggcg
     5701 taaggataaa acgcggccat caggtaggta tggtttttcg tcagggcggg aagatagttg
     5761 acgagtaaac tgttcaccgt ggcatagcgg tcgcttcgga acgacatgtt gtcgatgcgc
     5821 ttggcaccca ggacgaatcc gaatcctttc ttcgtatagg acgcattcaa caagagcgcc
     5881 tgaccttcct taaagataaa gccgttgctg gcggaagggt cattggcttt ataggcatac
     5941 tcggcactgg ccgaccatcc gccattcgta aatgaaacgc gtccggatcc cgaaccgaca
     6001 ttctccggca agcggaacgt gggatcctca tccgactgat atttgctaac gtaattgccg
     6061 ccaatgatcc actgggtttt tgctgcggcc cgcgactcgc aggattcgtt caccacccac
     6121 tctacatcac caccacgcac gatgccctgg cccaaattga aaaagtcacg ttgcttgccg
     6181 atcactcctt tgaaatagac gcccttcgca ggattataca tgaaacgaaa tccgtcgatg
     6241 gcgttgtcgt agcccagtcc ccgctcctca taggcacgaa gcaggatgcc gcttccgaac
     6301 tgttcgtaga aggtgccggc ggtgatatcc agttcattgt tcttgtaacg gaggaaccgg
     6361 tacggaatgc cgcttccttt gtagcgtttg tcgaaaccga gaatcgggtt ctgatacgat
     6421 tcataacgca ggccggccga aaaattgtct ttctcgtagg taaagttggc gaaaccgttg
     6481 aacagcatct gctccggtac gtccggtgca ccgatcaggc tgtcctcaaa ataatactgc
     6541 gcgtccaact gaacattacc gtgaataggc agattgggta acccctgggc tttgctcgcg
     6601 ccggccaggg cgaacaaacc caacagacat agaatacgct tcactggagg tggattaggt
     6661 gtggaatggt cagcccgaag gtaccgtata caagtttatg gaaaaaggcc ggcggcagga
     6721 tggttcgaaa tacggcaata aaaaaagccg cggttgcggc ttttcaaacg agctggatag
     6781 cccggtcact tcttttcgtc gaggggctgg ccagcggcca ctttcttgac aacttcatac
     6841 aattccaact cattcccttc ggcaaaaccg acgtgttgcc agacgatctc tccctttccg
     6901 ttcaagacaa atgtgtgcgg cggcatgttc acgttcaatg cccgggcgag atccttgttg
     6961 gggtcgacgt aattctcata gttccatcca cggccattca cgaatggcgc cacgcgcgtc
     7021 atggtacgct gatcatccat ggaaacggcg atcagtttca caccggtttc cttttgccag
     7081 tcggcgtaca gatcatgaat ggtcgtcaac tcctcgatgc acggcttaca ccaggttgcc
     7141 cagaaatcga tgatgatggg cttcccgtcg tttgcgatcg tttcggtatt caccgtctca
     7201 ccactgaggg tctttacatt gactttgggc aatggccgaa cggtggccgg ctggtcctgc
     7261 gcaaaaccgg taaaagcggt caagaacaga aggacaacta ctaggctttt tttcattttt
     7321 tcgatccgat aagtgataga ggccggatgg caattttttc aatttctgga gccctccagc
     7381 aaattccagt ccgaaataag gcaaacgcgg tcggattccc tatgccggtt tatcacagat
     7441 ttgttaaaaa gctggaattc aaagcgaaat cagcccaatg ccgttggatt tttcccaaaa
     7501 ttttacttgc tttggtgaac tgctataaaa gctaaccaac caaacaccaa ctgcgaaatg
     7561 aaaaaactgt tactaagtct tgtcgcgttg tttgccctcg gtaccgccac tgtgaacgca
     7621 cagatggccg ccggtaacat cgcacccgat ttcaccggta ccgatatcaa cggtaacacc
     7681 tggaacctgt attccctgct cgatcagggt aagaccgttt acatcgacgt atccgccacc
     7741 tggtgcggcc cttgctggaa ctaccacaac ggtcatgcgc tggaagacct ctatgctgcg
     7801 cacggcccga ccggtaccac atcacaggat gtaatggtct tcttcgttga aggggacgct
     7861 tctaccaccc tggccgacct gcaaggaacc ggcggtaata cccagggcga ctgggtgacc
     7921 ggcactcctt atccgatcat tgacgacgct accatcggtg acgcacttca gatcagctac
     7981 ttcccgacga tctacatcat ttgcccggat cgtatcatca aggaagtcgg acagttgacc
     8041 gccactgcat tgtataatca gcgtaactcc aactgttcgt tcgcaacctc gaccgatgat
     8101 gccggcatca ccaattcctt caccgccctc aacggaaccc tggcgacctg cgacgtggtt
     8161 gacctcgact accgcctgtg caactacggt actactccgc tgaccagtgc cacgatcgat
     8221 ctcaccgtga atggaactgt tcaacagtct tacaactgga
//
LOCUS       DAOTON010000017         8094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_7955_length_8094_cov_3.232367, whole genome shotgun sequence.
ACCESSION   DAOTON010000017 DAOTON010000000
VERSION     DAOTON010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7955_length_8094_cov_3.232367"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="P5292_00660"
     CDS             <1..1507
                     /locus_tag="P5292_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRS37664.1"
                     /translation="INAAAEADAKTGGKGGPGSAGKVLMATVKGDVHDIGKNIVGVVL
                     ACNNYQVIDLGVMVPAEKILEAARKEQVDVIGLSGLITPSLDEMVHVAKELEREGFKT
                     PLLIGGATTSRVHTAVRIEPHYTGPVVHVNDASRSVPVVSALLSREQREGFVQSQRTD
                     YARVREQHKQANAADKYLQLQLARENAFVSNHLDQPVPKPHRLGVTVLDDYPLAELVP
                     YIDWTPFFHSWELKGSYPKILQDPERGAEAKKLFDDAQAMLKRIVEERWLQARAVIGL
                     FPASREGDDVLVFDPEGKHTRCTFHTLRQQTKKPAGQHNLALADFVREPVIPGTPADY
                     VGAFALSTGFGIDERVAAFERDHDDYSAILLKALADRLAEAFAERLHERVRKEFWGYA
                     SDERLSNEELIGESYRGIRPAPGYPAQPDHTEKRTLWSLLDVEKNTGIQLTESLAMFP
                     TAAVSGLYFAHPDAHYFGIGRIQRDQVADYARRKGLSLEEMERWLGPNLAY"
     gene            1614..2681
                     /locus_tag="P5292_00665"
     CDS             1614..2681
                     /locus_tag="P5292_00665"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRS37665.1"
                     /translation="MSLSCSVGTDISRAARLLKRGKLVAIPTETVYGLAAHAFHPEAV
                     RAIYTAKGRPSNNPLILHTDHPERLPDWGMVLPPLAEELAREFSPGPLTFVIPASRRV
                     IPEVNGGQAGVAVRFPAHPLALELLQQLDFPLVAPSANPSGYMSPTRAGHVLEGLGDR
                     IDYILDGGPCAIGLESTILSFLEPQPVLLRHGGVPLELIEERIGTVRLHPSLWKEAAS
                     IDQHRLQEAPGMLSRHYAPRCPLQIGDPRERSGKYPGEKLARIFFCLPTGPLPERSWV
                     LSPEGSMGEAARNLFHVLRLAEQTDPDRIIVEPFPDEGIGRALNDRLRRAAFERTGAE
                     PNVFTSPPVPLSPRPARAAAR"
     gene            2849..3889
                     /locus_tag="P5292_00670"
     CDS             2849..3889
                     /locus_tag="P5292_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37666.1"
                     /translation="MNSISKKKLSYRINEELFMYLEEFGRLEELPFRYPDLLRYTYSV
                     PLYDKYGKDTLWETVYFQPTEMEELNQRLVKTYAMMKVEGNISLMEHLYVDRIDYCPF
                     GNSRPFRIRIKNQYNDNYDYFYVKLADASRVYGLELEDLLSPNRINYLVDADTLIEEH
                     IVGIPGDLFIKNQLNDQAVNPTRLAKEFIKFNERCFVRLLGDMRSYNFVIDMTADFDD
                     TQFRFRAIDFDQQCYEGKKSLYLPQYFKENNPYVNLCVKFINRETARQYQLEERTLMA
                     RRVKTARFRVASLLDVMIRDEISLKVKVEQLREEMAHHYHRPQFNKSKSMGDILRISL
                     DQLFEPDTGFRD"
     gene            3992..5416
                     /locus_tag="P5292_00675"
     CDS             3992..5416
                     /locus_tag="P5292_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS37667.1"
                     /translation="MRRIGFTFFLLLTGLSLRAQQAWTLQQCIDHALSHNLQVRQSEL
                     SVDQNKSQVQQSTATLFPSVNGSASQSYFFGRSIDPFTNVFANQQITNNNFNLNASMS
                     IFEGFQLMNTLRQSKLNYMASRADLDKIRNDIALNVATAYLQVLYNEDLVGVAEGQLE
                     SSRQQRDRAKRQYELGAIAQSGYLDLESQFATDDLNLVNARAQLAQSRLSLTQLLELD
                     SVSNFQITRPDVLLPAWDATSASVEVVFNAAMTNQPEIRASEYRYLSAERGLSIARGT
                     RYPRLSVSGNLNTNISSSAQEVTGFLALPPSIVESGFTSGGDTVYSVVPNNVIQTRET
                     PFSKQFEDNLGKSVGFTLSVPIFNGWAARNNVTRARIGLEQARISREQTRKTLYKSVQ
                     QAVTDAQAASSRYESGNRSLSALERSFEENRKRFEVGAISTYDFLLVQNNLARARATA
                     LQNKYDFLFRLKVLDFYQGKPLSF"
     gene            5431..6843
                     /locus_tag="P5292_00680"
     CDS             5431..6843
                     /locus_tag="P5292_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRS37668.1"
                     /translation="MAKKKSNLMKWLLITVAVLIVFAIVGKKAGWIGGAEEKEVAVEK
                     AEKRNIAETVSASGKIQPEVSVKISPDVSGEIVELPVKEGDRVKKGQLLVRILPDIYQ
                     SYVDRSVAALNGSKANFENAKSRLVQARSQFDKAKQTYDRSKKLFDEKLISPQEWEGV
                     KSAYEVAQAEVSASEQSVSAADYSVRSSEASLKEAQDNLRKTSIFAPVDGTISKLNVE
                     KGERVVGTSQMAGTEMMTLADLNEMEVSVDVNENDIVRVNTGDTATIEVDAYMGKKFK
                     GVVTEVANSANISGLTVDQVTNFTVKVRVLRESYEQMADPEHPERSVFHPGMSATVDI
                     MTKRVSDVISVPIQSVTTRDTTEKGGGTTGMKGGDNKMEDETEITVKDEKAEKAEKAD
                     QLDESDKRVECVFLLDNGKVRLVPVKTGIQDNSYIEIKAGLKVGDQVVSSPYSAIAKF
                     LKDGDPVKVVPKEQLFNKKD"
     gene            6897..7598
                     /gene="tsaB"
                     /locus_tag="P5292_00685"
     CDS             6897..7598
                     /gene="tsaB"
                     /locus_tag="P5292_00685"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRS37669.1"
                     /translation="MPTLLHIETATAVCSAAISRDGKLLALRETLEPQAHAAMLAVFI
                     RELFDEIGIQAPDLDAVSYSSGPGSYTGLRIGISTAKGLCFAADKPLIAIPTLDALAF
                     AATSVVQAGDRIIPMLDARRMEVYTQVYDFSGHPESGPEALVLDEQAFAALLAQGPVW
                     FLGDALTKARPLLSAHPNARFLPDVLCSAQHHAQPALRAWEDRRFEHLGLSEPFYLKE
                     FVAGKGSVLPGPAQG"
     gene            complement(7538..>8094)
                     /locus_tag="P5292_00690"
     CDS             complement(7538..>8094)
                     /locus_tag="P5292_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRS37670.1"
                     /translation="ALLLFLVPTHAALFERQMREWTARWPACRFIGWTAAHRPDDSDI
                     FRLLNAGVQALLPETSDSEDLYRACRDVLQGDIHLNCHVTQAMLHFLRRQQILKTKSA
                     DGLMSPRECRMVELRREGKTAEEIADRLCLSRKTVDKLFCELYRKTGTRNFFELLKEY
                     EQRAYQPWAGPGSTEPFPATNSLR"
BASE COUNT         1805 a         2450 c         2223 g         1616 t
ORIGIN      
        1 catcaacgcg gccgcagagg ctgacgcgaa gacgggaggg aagggcggcc ccggcagcgc
       61 gggcaaggta ctcatggcta ccgtcaaggg cgatgtacac gacatcggca agaacatcgt
      121 cggtgtggtc ttggcctgca acaactacca ggtcatcgac ctgggggtca tggtgccggc
      181 cgagaagatc ctggaagccg cgaggaagga gcaggtggat gtcatcggcc tcagcggact
      241 catcacccct tcgctcgatg aaatggtgca cgtggccaaa gaacttgaac gggaaggctt
      301 caagacgccg ctgctgatcg gcggcgctac gacttcgcgc gtgcacaccg ccgtgcgcat
      361 tgaaccacac tacaccggac cggtcgtgca cgtgaacgac gcgtcccgca gcgtacccgt
      421 tgtcagcgcc ctgctttccc gggagcagcg ggaaggtttc gtgcagtcgc agcgaaccga
      481 ttatgcccgt gtccgcgaac aacacaagca ggcgaacgcg gccgataagt atctccaatt
      541 gcagctcgcg cgcgagaatg ccttcgtcag caaccacctc gaccaaccgg tgccgaaacc
      601 gcatcggctg ggcgtgacgg tgcttgacga ttatccgttg gccgaattgg ttccgtacat
      661 cgactggacg cccttcttcc atagctggga gttgaaaggt tcgtatccga agatcctgca
      721 ggatccggaa cgcggcgcgg aagcgaagaa actgttcgac gacgcacagg cgatgctgaa
      781 acgcatcgtg gaagagcgct ggctgcaggc ccgtgccgtg atcggtctgt tccccgcttc
      841 ccgcgaaggc gacgatgtgt tggttttcga cccggaagga aaacacacac gctgtacttt
      901 tcataccctg cgtcagcaga caaagaaacc ggccgggcag cataacctgg cgctcgctga
      961 tttcgttcgc gagccggtga tcccggggac acccgccgac tatgttggtg ccttcgcgct
     1021 ctcgacagga ttcggtatcg acgaacgcgt cgctgcgttc gaacgcgacc acgacgacta
     1081 ttccgccatc ctcctcaaag cactcgccga tcgcctggca gaagcttttg ctgaacgact
     1141 gcacgagcgt gtgcgcaagg agttctgggg ctacgcctcc gacgagcggc tcagcaacga
     1201 agaactcatc ggcgagtcct accgcggcat ccgtcccgca cccggttacc cggcgcaacc
     1261 cgaccatacc gagaaacgca cgctctggtc cttgctcgac gtggagaaga ataccggcat
     1321 tcaactcacc gaaagcctgg ccatgttccc caccgctgcc gtgtcgggtc tctacttcgc
     1381 acaccccgac gcgcactact tcggcattgg tcgcattcag cgcgatcagg tcgccgatta
     1441 cgcgcgacgc aaaggcctct ccctcgagga gatggaacgc tggctaggcc cgaacctggc
     1501 gtactgagca ctcccatgca ggaggcccgg tcgaaaaatt tgaaaatgat agactcaatc
     1561 tttttaaaga tagtaatact attatttttc aaaaatgccc gtaactttgt ggcatgtcgt
     1621 tgtcctgttc ggtgggtacg gatatcagtc gggccgcccg cctgctcaag cgcggaaaat
     1681 tggtcgcgat cccgactgaa accgtgtatg gcctggcggc tcatgcgttc catccggaag
     1741 ccgtacgcgc gatctacacg gcgaaaggac gcccctcgaa caacccgctc atactgcata
     1801 cggatcatcc cgaacgttta ccagactggg gaatggtcct gccaccactc gccgaagaac
     1861 ttgcccgtga attttctccc ggtccgctca cttttgtcat ccctgcttcc cgtcgggtta
     1921 taccggaggt gaacggcgga caagccggcg ttgccgtgcg cttccccgca cacccgcttg
     1981 cgttggaatt gttgcagcaa ctcgatttcc ccctcgtagc accgagcgcg aaccccagcg
     2041 gatacatgag cccgacccgc gccgggcacg tgctggaagg cctcggcgat cgcatcgact
     2101 acatcctcga cggcggcccg tgtgccatcg gactggaatc caccatcctt tcttttctcg
     2161 aaccccaacc cgtactgctt cgtcacggcg gagtaccgtt ggaactgatc gaggagcgta
     2221 tcggtactgt gcgcctccat ccatcactgt ggaaggaagc tgccagcatc gaccaacacc
     2281 gcctgcagga agcacccggc atgctgagtc ggcattacgc gccacgctgc cccttgcaga
     2341 tcggtgatcc ccgcgaacga tccggcaagt accccggcga gaaattggca cgcatctttt
     2401 tttgtttacc gaccgggcca ctacccgagc gatcctgggt gctttctccc gagggcagca
     2461 tgggagaagc cgcgcgcaac ttgttccacg tcctccgact cgccgagcaa accgaccccg
     2521 accgcatcat cgttgaacct ttccccgacg aaggcatcgg tcgcgccctc aacgaccgtt
     2581 tgcgcagagc cgcatttgaa agaacaggag cggagccgaa cgttttcaca tcgccacccg
     2641 tcccgctctc gccccgtccc gcccgggcgg ctgcccgttg atattcgact ggcgatggcg
     2701 tgccggggct ccatcggggc tggcctggaa cgtctccggc ttcgagccgc tgacaaaccg
     2761 gcgatgaggc atatccgcag gctgttgttt gaaaactccg cagcctgcag cgtgaacccc
     2821 gcctcccttg ccgcatcttc gtagcgggat gaattccatt tcaaagaaga agctcagcta
     2881 ccgcatcaac gaggaactgt tcatgtacct cgaagagttc ggccggctcg aagaacttcc
     2941 gttccggtat ccggacctgc tccggtatac ctacagcgta ccgctctacg acaagtacgg
     3001 gaaggacacg ctctgggaaa cggtctattt ccagcctacg gaaatggagg agctcaacca
     3061 gcgactcgtg aagacctacg ccatgatgaa ggtggaaggg aacatcagcc tcatggagca
     3121 cctgtacgtc gaccgcatcg actactgccc cttcggcaac tcgcggcctt ttcgcatccg
     3181 gatcaagaac cagtacaacg acaactacga ctacttctat gtcaaactcg ccgatgcttc
     3241 gcgcgtctac ggattggaac tggaagatct cctctcgccg aaccgcatca actacctggt
     3301 cgatgccgat acgctcatcg aagaacacat cgtcggtatt cccggcgacc tctttatcaa
     3361 gaaccagttg aacgaccagg ccgtcaaccc gacgcgcctt gccaaggagt tcatcaagtt
     3421 caacgaacgc tgcttcgtac gcctgctcgg cgatatgcgg tcctacaact tcgtcatcga
     3481 catgaccgcc gatttcgacg atacgcagtt ccgcttccgc gcgatcgact tcgaccagca
     3541 atgctacgaa gggaagaagt cgttgtacct gccgcaatat ttcaaggaga acaatcccta
     3601 cgtcaacctc tgtgtcaagt tcatcaaccg ggaaaccgcc cggcagtacc agctcgaaga
     3661 gcgtacactc atggcccgcc gggtcaagac cgcgcgcttc cgggtggcgt ctttgctcga
     3721 cgtgatgatc cgcgacgaaa tttcgctcaa ggtgaaggtc gagcagttgc gcgaagagat
     3781 ggctcatcat taccaccgcc cgcaattcaa caagtccaaa agcatgggcg atattctcag
     3841 gatctccctg gatcaactct tcgaaccaga tacgggcttc cgggattgat tccgaaatgc
     3901 ctttttttaa cgttaatcta cgttaagggc ttccaaatgt taaaattgat tcccttactt
     3961 tgacccgtcc ctgatccttt accccttccg catgcgacgt atcggtttca ccttttttct
     4021 gctcctaacc gggctttccc tgcgtgcgca acaagcctgg acgctgcagc agtgtatcga
     4081 ccatgccttg tcgcacaacc tgcaggtgag gcaatcggaa ttgagcgtcg accagaacaa
     4141 aagccaggta cagcaaagca ccgccacgct attcccctcg gtgaatggtt ccgcttccca
     4201 gagctatttc ttcggccgaa gcatcgaccc gttcaccaac gtcttcgcca atcagcagat
     4261 caccaacaat aacttcaacc tgaatgcatc gatgagcatc ttcgaaggct ttcagttgat
     4321 gaacaccctg cgccaaagca aactgaatta catggcgagc agggcggacc ttgataagat
     4381 ccggaacgat atcgcgctca acgtcgcgac ggcttacctg caggtgttgt acaacgaaga
     4441 cctcgtcggc gtagccgaag gacagttgga atcgtcccgc cagcagcgcg accgggcaaa
     4501 acgccagtac gaactcggtg ccatcgcgca gtcgggttac ctggatctgg agtcccagtt
     4561 cgccaccgat gacctcaacc tggtgaacgc acgcgcgcaa ctggcacaat cccgcctttc
     4621 cttgacccaa ctcctggaac tggattcggt cagcaatttc cagatcacgc gtcccgacgt
     4681 cctgctgcct gcctgggacg ccacttccgc gtcggtcgag gtcgtcttca acgccgccat
     4741 gaccaatcag cccgagatcc gtgcttcgga atatcgctac ctcagcgctg agcgcggact
     4801 gtcgatcgct cgcggcacac gctatccgcg actttccgtg tccggtaacc tcaacaccaa
     4861 catctccagt tctgctcagg aagtaaccgg ttttctggcc ctgcctccct cgatcgtgga
     4921 gtcaggattc acctccggcg gcgatactgt ttattcggtt gttcccaaca acgtcattca
     4981 gacccgggaa acacccttct ccaagcaatt cgaagacaac ctgggtaaat cggtcggctt
     5041 tacgttgtcg gttccgatct tcaacggctg ggccgcgcgc aacaatgtta cgcgtgcccg
     5101 gatcggactc gaacaggccc gaatcagccg tgagcagacc cgcaagacac tctacaaatc
     5161 cgttcagcag gcggtaacgg atgcccaggc agcttcgtcg cggtatgaat ccggcaaccg
     5221 cagcttgtcg gcactcgaac gttccttcga ggaaaaccgg aaacgcttcg aggtaggtgc
     5281 gatctctacc tatgacttcc tgctggtgca aaacaacctg gcccgcgccc gtgcaacggc
     5341 cttgcagaac aaatacgatt tcctgtttcg tttaaaagta cttgacttct accagggcaa
     5401 accgttgtcc ttctaatcac cccttcgcac atggccaaga aaaagtccaa cctgatgaaa
     5461 tggctcttga tcacggtagc cgtcctgatc gtctttgcca ttgtcggcaa gaaagccgga
     5521 tggataggag gagcggagga aaaggaagta gccgtggaga aagccgaaaa gcgtaacatc
     5581 gccgaaaccg tatcggccag cggaaaaatc cagcccgagg tatccgtcaa gatcagcccg
     5641 gacgtttccg gagagatcgt cgaactgccg gtgaaagagg gcgaccgtgt gaagaaagga
     5701 caactgcttg tccgcatcct accggacatc tatcagtcgt atgtcgatcg ctccgtagcg
     5761 gctttgaacg ggtcgaaagc caatttcgaa aatgccaaat cccgcctggt gcaggcccgc
     5821 tcccaattcg acaaagcgaa gcagacctac gatcgcagca agaaactatt cgacgagaag
     5881 ctgatctccc cgcaagaatg ggaaggtgtg aaatccgctt acgaagtggc acaagccgaa
     5941 gtctccgcat cggagcaaag tgtttccgcc gccgattaca gcgtacgcag ttcggaggct
     6001 tccttgaaag aggcgcagga caacctgcgc aagacctcca tcttcgcccc ggtcgacggt
     6061 accatcagca aactgaatgt agagaaaggc gaacgggtcg tgggtacttc ccagatggcc
     6121 ggaaccgaga tgatgacgct ggccgatctg aacgagatgg aagtcagtgt cgatgtgaac
     6181 gagaacgaca ttgttcgggt caataccggc gatacggcta ccatcgaggt ggatgcctat
     6241 atggggaaga agttcaaagg tgtggtaacc gaagtggcga attccgcgaa catctcggga
     6301 ctgaccgtgg atcaggtcac caacttcacc gtcaaggtgc gcgtactgcg cgagtcgtat
     6361 gaacagatgg ccgatccgga gcatcctgaa cggagtgttt ttcatccggg catgtcggcc
     6421 acggtcgata tcatgaccaa gcgggtttcc gatgtcatat ccgtaccgat ccaatcggtc
     6481 accacgcgcg atacgaccga gaaaggcgga ggcacgacgg gtatgaaagg cggagacaat
     6541 aaaatggagg acgagaccga gatcaccgtc aaggatgaaa aggcggagaa agcggaaaag
     6601 gccgatcagt tggatgagtc ggataagcgt gtcgaatgcg tctttctgct ggacaacgga
     6661 aaagttcgac tcgtacctgt gaaaaccggc attcaggata acagctacat cgagatcaaa
     6721 gccggcctga aagtcggcga ccaggtggtt tcgtcccctt acagcgcgat cgcgaagttt
     6781 ctcaaggacg gcgatcccgt gaaagtcgta cccaaagagc agttgttcaa caaaaaggac
     6841 tgacgctgat cctgagcgaa tcaaaaccgg gtgttacctt tacacccggt ttttttatgc
     6901 ccacactcct gcacatcgag accgcgacgg cggtatgttc cgcggccatc agccgcgacg
     6961 gcaagctcct ggcgttgcgg gaaactttgg agccgcaggc gcatgcggcc atgctggctg
     7021 ttttcatccg ggaacttttc gacgaaatcg gtatacaggc acctgacctg gatgcggtca
     7081 gctatagttc aggtcctggt tcatataccg gtctgcggat cgggatcagc acggcgaaag
     7141 gactttgctt cgctgccgac aagcccctca tcgcgatacc cacgctggat gcgctcgcct
     7201 tcgccgcaac ctccgtcgtg caggcgggtg atcgcatcat cccgatgctc gacgcccgac
     7261 ggatggaagt gtacacgcag gtgtacgact tcagcggcca tcccgaatca ggacctgaag
     7321 cacttgtgct cgacgaacag gctttcgctg cgttactggc gcaggggccg gtgtggtttc
     7381 tcggcgatgc gttgaccaaa gcgcgcccct tgttatcggc tcatccgaac gcccgcttcc
     7441 tgccggacgt gctgtgttcc gcgcagcatc atgctcaacc ggcgctgcgg gcctgggaag
     7501 accgacgatt cgagcacctc ggactttcgg agccgtttta tctcaaagag ttcgtagccg
     7561 gaaaaggctc cgtactcccg ggaccggccc agggttgata ggcccgttgt tcgtactctt
     7621 tcagcagttc gaaaaagttc cgggtacccg ttttcctgta aagctcgcag aagagtttat
     7681 cgaccgtctt ccggctcagg cagaggcgat cggcgatctc ttccgcggtc tttccttccc
     7741 gccgcagttc gaccatgcgg cattcacggg gactcatgag accgtcggcg ctttttgttt
     7801 tcaggatttg ctgccgccgg aggaaatgca gcatcgcctg cgtcacatgg cagttgaggt
     7861 ggatgtcgcc ctgcaacacg tcgcgacaag cccggtagag gtcttcgctg tcggaagttt
     7921 cgggtaacag ggcctgtacg cccgcattga gcagtcggaa gatgtctgaa tcgtcgggac
     7981 gatgcgcggc ggtccagccg atgaaccggc aagcgggcca gcgtgctgtc cattcgcgca
     8041 tctgtcgttc gaacaaggca gcatgcgtcg gcacaagaaa caggagtagt gccg
//
LOCUS       DAOTON010000018         7803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_8264_length_7803_cov_3.538849, whole genome shotgun sequence.
ACCESSION   DAOTON010000018 DAOTON010000000
VERSION     DAOTON010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8264_length_7803_cov_3.538849"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1429
                     /locus_tag="P5292_00695"
     CDS             <1..1429
                     /locus_tag="P5292_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRS37671.1"
                     /translation="RPYGIHNAAVLVVNNATRAVEAYVGSPDFDDNGHGGQVDGVRAV
                     RSPGSTLKPLVYGIAFDLGLLTPKRIIDDVPVNFGGYAPDNFDEHFSGPVTIEDALAR
                     SLNIPAVKTLQQIGLATMIDRMRTAGFEQARHQEKSLGLSLVLGGCGVRLEELTALFA
                     ALADSGYYAPLRYLADDSVRTGVPVIGEGAAEMLRDILVLPNRPDLPASAGERSRSVR
                     IAWKTGTSYGRRDGWSIGYNPRYTIGVWVGNFSGEGVPELTGADRATPLLFELFHALD
                     YNSAGTWFKPGKGTDFRLVCKESGQVPSEHCAETVIDQFLPGISSVVRCGHEVEIATD
                     PRGSISYCTSCKPETGYRMQWFPNPAADLARFYDSEQIPYIKIPPHNPACTRVQREQA
                     PVIVSLQDGKEYLIEQDGGEQLPLNAQPANDVRQHYWYVNDRFVGACEPSETVFLKPL
                     AGTSKISCSDDKGRNRDIRISVRFY"
     gene            complement(1410..2411)
                     /locus_tag="P5292_00700"
     CDS             complement(1410..2411)
                     /locus_tag="P5292_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRS37672.1"
                     /translation="MLQWSIEPVRLELRYTWKISRNATDEKTNLIVTLADGSVEGQGE
                     AAPNVRYGESPERLQEEFLRFVSATGPEQRTPEAYVRIARASGCSNALRFAIESAAWH
                     FASGNRREELYRALSLDAPGRVPTSYTIPIIDPGQLRAFYDREQLSRFRYLKLKIDRE
                     SAYDSIAELCTFTKAPVMVDANEAFTDVEELIRLLEKIRRLPIEFVEQALPDKLREES
                     LYLKKYCPFPHFADEAVTDEPDMAFISAAYHGVNMKLMKAGGYSNGLRILREAKRLGL
                     RTMIGCMVETSLGIGSALNLCSLADYADLDSFLILKKEPYGLIREEDGLLSFSRTEH"
     gene            3107..5131
                     /locus_tag="P5292_00705"
     CDS             3107..5131
                     /locus_tag="P5292_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRS37673.1"
                     /translation="MITSFQETLRSQALRNQRSLNVFSAIVVTILGFLFEFAYHDGYI
                     LISGLLVSMLMTSNYFLSYYNDFYRNKLRQITELSVFILHFWALFVAYQRNFEIDVLL
                     PVAISTFTFSLVFDKFFRSLIFLFTITTVLLILMLTTHHWRPEYTITLVSLYAGAFLS
                     DQILKRKKQYHGILEKQEERYISLVENMNDGLVYIDNDRTVQFVNDTFCQISGYTREQ
                     VIGKDLIEFAPEAQLDSPNSFYRRLREGNNVRVESELSKWSGQVIRVQKSGAPYFGEG
                     RKRMGSMVVFTDISGLYDAREQLKKSEEGYRTFIEQSAVGIWRAEYRQPIPVDLPIDQ
                     QVELLLDTGIIVECNDFMARMYGHSDRADLIGRRIRDFYYIENNLDEQKTRELMSAYI
                     QNNYRVSNAESKELDKHGSIRYMLNNNIGIVENGHLMRTWGVQTDITDRKRTERELLE
                     TNQELDTFFYKASHDLKGPLASVMGIVNLARIESRDQIMSNYFDMIETSIKRLDQTLL
                     DLIELARTRKGASKLSSIRVHDLVGEILHSLRHLPHYSRINFELKIDDQLEIISDKVL
                     VLSVFQNLIHNAINYCNPDSPWIRIRVDGNPNGINIEITDNGKGIPEKIQGRVFEMFY
                     RGNPDSSGSGLGLFIVKNALEKLKGKIRFISAPGRGTSFYVEIPSALSES"
     gene            5145..5549
                     /gene="mce"
                     /locus_tag="P5292_00710"
     CDS             5145..5549
                     /gene="mce"
                     /locus_tag="P5292_00710"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRS37674.1"
                     /translation="MFLKIEHLGIAVRNLESANKLFAALLGTEHYKQEAVEREKVTTS
                     FFQMGGSKIELLEASDPESPIAKFIEKRGEGIHHIAFEVEDIRATIKRLKEEGFHLLS
                     DEPKPGADNKLIAFLHPKSTNGVLVEICQERH"
     gene            complement(5553..6251)
                     /locus_tag="P5292_00715"
     CDS             complement(5553..6251)
                     /locus_tag="P5292_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRS37675.1"
                     /translation="MRLRRYIFASIPEIPRPVRCSNDSMPIRNLILDLGGVLFAIDYR
                     RPVDAFRKLGFPEFQRQFSQQEQHHFFDDFEKGLITIPVFRDRLRSFTSLPVSDQAID
                     AAWNSILVGFPDDSRQLLERLQGKYRLFLLSNTNALHEIAFRKMIREQHGADLLDELF
                     EKVYLSHRIHQRKPDRDAFDLVMNDAGLDPDETFFVDDSPQHVKGAELAGIHAAWLEL
                     PKSTTILLEELKLL"
     gene            6281..6354
                     /locus_tag="P5292_00720"
     tRNA            6281..6354
                     /locus_tag="P5292_00720"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6315..6317,aa:Ile,seq:cat)
     assembly_gap    6547..6646
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6647..7123
                     /locus_tag="P5292_00725"
     CDS             <6647..7123
                     /locus_tag="P5292_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRS37676.1"
                     /translation="EQMNATNHFGTAFGLGMSSLRVDHNAMAATDTDGVGTVLVPIPD
                     SLDYKQNRYVMNSIDAAFEFRFRGNPNKNHKHFKFSLGFRAGYVIQSHLKYKDEDVKY
                     KLYSIEDIQRWRYGVTGRIGLGHLALDAYYSLAPLYKEGKGPADMMPYSIGIAWTF"
     gene            complement(7128..7736)
                     /locus_tag="P5292_00730"
     CDS             complement(7128..7736)
                     /locus_tag="P5292_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37677.1"
                     /translation="MSRTFAQFLSILLHPVLMPTYAIWFLFHHSSYFSYTVSPPERDA
                     LYMIVLLNTLVLPTIVSYILSRRGWIRSFEMEKREERVIPFLTNAALLLIAYYLMRKL
                     MLPQVFPLLILGAAVAVTFAAIINFKWKISIHMIGIGGIIGTFFGLSTFLLVDVRIPI
                     LFCLLVAGLLGTARLSLGAHTPMQIYAGFLVGFLCEFLLLSI"
BASE COUNT         1789 a         2055 c         2132 g         1727 t
ORIGIN      
        1 ccgaccttac ggcatccaca acgcggcggt ccttgtcgtc aataacgcaa cccgggcggt
       61 ggaagcgtat gtcggctctc ccgacttcga cgataacgga catggcggac aggttgacgg
      121 agtacgtgcg gtacgctcgc cgggatccac gctcaagccg cttgtgtacg gtatcgcctt
      181 cgatctcggc ttattgacgc ccaagcgcat catcgatgat gtgcccgtca acttcggtgg
      241 ttatgctcct gacaatttcg acgaacactt tagcggccct gtaacaatcg aagacgcgct
      301 cgcccgttcc ctgaatatac cggcggtaaa aacgctgcag cagatcggat tggctaccat
      361 gatcgaccgg atgcgtaccg cgggattcga acaagcccgg caccaggaaa agagtctcgg
      421 gctttctctt gttttgggcg gttgcggtgt tcgcctggag gaactgaccg ccttgttcgc
      481 cgcgctggcg gattcgggtt actatgcgcc gctgcgttat ttagcggatg acagtgtgcg
      541 caccggtgtt ccggtgatcg gtgaaggcgc ggcggagatg ctccgcgaca tcctcgtact
      601 ccccaatcgc cccgacctgc ccgcctcggc cggagaacgt tcgcgctccg tgcgtatcgc
      661 ctggaagacc gggaccagtt atggccgccg cgatggctgg agtatcgggt ataatccgcg
      721 ctatacgatc ggtgtttggg tcgggaattt ttccggcgaa ggagtacccg aactcactgg
      781 agccgaccgt gcgacacctt tgctgttcga gttgtttcat gcgctggact ataacagtgc
      841 cggcacctgg ttcaagccag gaaaggggac tgacttccgc ttggtttgta aggaaagcgg
      901 ccaggtgcct tccgaacatt gcgcggagac ggtcattgat cagtttttac cgggcatttc
      961 ctctgttgtg cgatgtgggc atgaagtgga aatagccacc gacccgcgcg ggagcatttc
     1021 gtattgtacc agttgtaaac cggagaccgg atatcgaatg cagtggtttc cgaatccggc
     1081 tgcggacctg gcccgattct atgattccga gcagatccct tacatcaaga taccacccca
     1141 caatccggcg tgtacacgcg tgcaacggga acaggctccg gtgatcgtat ccctgcagga
     1201 tggaaaagaa tacttgattg agcaggatgg cggcgaacaa ttgccgctca atgcccaacc
     1261 ggcgaacgat gttcgccagc actattggta cgtgaatgac cggttcgtcg gcgcgtgtga
     1321 accctcggaa acagttttcc tcaaaccgct ggcgggaacg agtaagatct cctgcagcga
     1381 cgacaagggc cgcaaccggg atatccgaat cagtgttcgg ttctactgaa gctgagaagt
     1441 ccgtcttctt cccggatcaa tccgtatggt tcctttttca agatcaggaa gctgtcaagg
     1501 tccgcgtagt cggccaggct gcaaaggttg agtgccgatc cgatgccgag actggtttcg
     1561 accatacaac cgatcatagt acgcaggccc aggcgtttcg cttcccgaag aatccgtaag
     1621 ccgttggaat aaccacccgc tttcatcagt ttcatgttca cgccatggta cgcggcgctg
     1681 atgaatgcca tatccggctc gtcggtcacc gcttcgtcgg cgaaatgggg gaaaggacag
     1741 tactttttca ggtaaagaga ttcttcccgc agcttgtcgg gcagggcttg ttccacgaat
     1801 tcaataggca ggcgacggat cttttccagc aaccggatga gttcttccac atcggtgaac
     1861 gcctcgttcg cgtcgaccat gaccggggct ttggtgaacg tgcagagttc ggcgatcgaa
     1921 tcataggcgg actcccggtc gatcttgagt ttcaggtacc ggaagcggct cagttgctcc
     1981 cgatcgtaaa acgctcgcaa ttgtccggga tcgatgatcg gaatggtgta cgaggtagga
     2041 acacgtcccg gtgcgtcgag cgagagtgcg cgatacagtt cttcccgacg gttgccgctg
     2101 gcgaagtgcc aggcagccga ttcgatggcg aagcgcaggg cgttggaaca accggaggcc
     2161 cgagcgatcc ggacgtatgc ttccggcgta cgctgttccg gacccgtcgc agaaacaaat
     2221 cggaggaact cttcctgtag tcgttccggg gattcaccgt aacggacatt cggcgccgct
     2281 tcgccctgac cttccacgga accgtcggcg agggtgacga tcaggttcgt cttttcgtcg
     2341 gtggcgtttc gggagatctt ccaggtgtac cggagttcga gtcgaaccgg ttcgatggac
     2401 cactgcaaca tgcggctgga tgagaatgac aaagcaaata aaacaaaagt ccctcgttcg
     2461 tgatgagcga gggactcttg cagaccgcta ctagagcggg aagcgttacg cttacttctt
     2521 cttcttagct gctttcttgg tagctttctt agctttctta gcagctttct ttgcttttgc
     2581 catagttaga agtttttgat tgttatgact actcaaacat aattaattaa aaaaggttac
     2641 actgtttgta cccaaaattc tatcaacatg aaattgttga tttcgttggc ggtgttttcg
     2701 tgctgcaatc atgtgcattc atgcgtgtcg caacgaagtt gatgatgctt gcaaagccgc
     2761 taccacagcg actttgcaat gctttgcaaa acatgtggaa ggtgatgcat cgcgtgtgac
     2821 gcgtgttgtt gtcgtgatcg cgaaatggag ttattcacaa tgacctcctg ccgaaaaaag
     2881 ttggcgaaga gatcaaagtt gcacgatcag cgaccggtga actactcatt tcgacccttt
     2941 ccatgtgctc cggttggcga ttcgtcaatc atctgtccat gatcgaacgg atcttgataa
     3001 ccactatacc tatatatata gtaacatccg gtcaaccgtc ggatcattca ctgttcgatc
     3061 cggctcattt cgttactttt gatcgttcgg tcacgcacta ccgggaatga tcaccagttt
     3121 tcaggaaaca ctacgatcgc aggcgctcag gaaccagcgg tcattgaatg ttttttccgc
     3181 catcgtcgtg acgatcctcg gattcctgtt cgagttcgcc tatcacgacg gttacattct
     3241 catttcggga ctactggtgt cgatgttgat gacgtcgaac tatttcctgt cttactacaa
     3301 cgatttctat cgcaacaagc ttcgccagat cacggagcta tcggtcttca tcctgcactt
     3361 ttgggccttg ttcgtggctt accagcgtaa tttcgagatt gatgtcctgt taccggtggc
     3421 catctcgacg ttcaccttta gcctggtgtt cgacaagttc ttccgaagcc tgatcttcct
     3481 gttcaccatc acgacggtcc tgctgatcct gatgctgacc acccatcact ggcgtccgga
     3541 atataccatc accctggttt ccctgtatgc cggcgcgttt ctgtccgatc agatcctcaa
     3601 gcggaagaag cagtaccatg ggatcctgga aaagcaggaa gagcgctaca tctcgctcgt
     3661 ggaaaacatg aacgacggac tggtctatat cgacaacgac cggacggtcc agttcgtcaa
     3721 cgataccttt tgtcagatct ccggctatac ccgggaacag gtcatcggca aggatctgat
     3781 cgagttcgcg ccggaagccc aacttgattc tccgaattcg ttctatcgcc gcctgcggga
     3841 agggaacaat gtccgggtag agtccgaact cagtaagtgg agcggacaag tgatccgggt
     3901 gcagaagtcc ggcgccccgt atttcggaga aggccgaaaa cgaatgggct ccatggtggt
     3961 gtttactgac atctcgggtc tatacgatgc gcgggagcaa ttgaaaaaat ccgaggaagg
     4021 ctaccggacg ttcatcgagc agagcgctgt cggaatctgg cgcgccgagt accgtcaacc
     4081 gattcccgtt gaccttccca tcgatcaaca ggtggagttg ctgctcgata ccgggatcat
     4141 cgtcgaatgc aacgacttca tggcccgaat gtatggacac tccgatcggg cggatctgat
     4201 cggacgcagg atccgggatt tctattacat cgagaacaac ctcgacgagc aaaagacgcg
     4261 ggagctgatg tcggcctata ttcagaacaa ctaccgggtc agcaatgcgg agtcgaaaga
     4321 actcgacaaa cacggcagta tccgctatat gctgaacaat aacatcggga tcgtcgaaaa
     4381 tggtcacctg atgcggacct ggggcgtcca aaccgatatc accgaccgga aacgaaccga
     4441 acgcgaactg ttggagacca accaggaact ggacaccttc ttttataagg cctcccacga
     4501 tctgaagggt ccgttggcca gcgtcatggg catcgtcaac ctggcgcgca ttgaatcccg
     4561 tgaccagatc atgtccaact atttcgacat gatcgagacc agcatcaagc gacttgacca
     4621 gacactcctc gacctgatcg agttggcgag aacgcgaaaa ggggccagca agctcagcag
     4681 tatccgcgtt catgacctcg taggcgagat cctgcactcc ctccggcacc tgccgcatta
     4741 ttcccggatc aatttcgagc tcaagatcga cgaccagctc gagatcatct cagataaggt
     4801 cctggtgctt tccgttttcc agaacctgat ccacaatgcc atcaactact gcaatccgga
     4861 cagcccctgg atcaggattc gggtcgacgg caatccgaac ggtatcaaca tcgagatcac
     4921 cgataacggg aagggaatcc cggaaaagat ccagggccgg gtgttcgaga tgttctatcg
     4981 cggcaatccc gattccagcg gttccggtct cggactcttt attgtaaaga acgccctcga
     5041 gaaactcaaa ggaaagatcc ggttcatcag cgccccgggt cgcgggacgt ccttctatgt
     5101 ggaaattccc agcgcgctgt cggaatcctg aaattaccaa tcgtatgttc ctgaagatcg
     5161 aacacctcgg catcgccgtc cgaaacctgg agtcggcaaa caaactcttt gcagccctcc
     5221 tgggtacaga gcattataag caggaggccg tcgagcggga aaaggtgacg acttcctttt
     5281 ttcaaatggg tggcagcaag atcgagttgc tggaggcgag cgatccggaa agcccgatag
     5341 cgaaattcat cgaaaagcgt ggcgagggta tccaccatat cgcgtttgaa gtggaagata
     5401 tccgggcaac gatcaagcgg ctgaaggaag aaggctttca tttgctgtcg gatgaaccca
     5461 agccgggtgc cgataacaag ctcatcgcct tcctgcatcc caaatcgacc aacggcgtgc
     5521 tggtcgagat ctgccaggaa cggcactgac cgttacagca atttaagttc ctccaacagg
     5581 atcgtcgtcg actttggcaa ctccagccag gcagcatgga tgccggccaa ttccgcgcct
     5641 ttcacatgtt gcggcgaatc gtcgacgaaa aaggtctcgt ccgggtcgag tcccgcatcg
     5701 ttcataacca gatcaaaagc gtcacggtcc ggcttgcgct gatggatgcg gtgcgagagg
     5761 tacacttttt caaacagctc gtcgagcaga tcggcaccgt gctgttcccg gatcatcttg
     5821 cgaaaggcga tctcatgcag cgcgtttgta ttgctcagca ggaagagccg gtacttgcct
     5881 tgcaaacgtt ccagtaactg tcggctgtcg tccggaaaac cgaccaggat gctgttccat
     5941 gcggcatcga tcgcctggtc ggaaaccggc aggctggtaa acgagcgcag gcggtcgcgg
     6001 aaaaccggga tcgtgatcag ccccttttca aagtcatcga agaaatgatg ctgctcctgc
     6061 tgggagaact gtcgctggaa ttcgggaaat ccaagtttgc gaaacgcatc aacgggccga
     6121 cgatagtcga tcgcgaagag taccccaccg aggtcgagga tcaggttgcg aatgggcatg
     6181 gagtcgtttg aacaccttac cggacgcggg atttccggaa tggacgcaaa gatatatctt
     6241 cgcaggcgca tccgcggtcg atttaatacc gactgtatga gggcccgtag ctcaattggt
     6301 tagagcagca gactcataat ctgcgggtcg ctggttcgat tccagccggg cccacgagcg
     6361 gaacacaatg cccggaaagt tccgggcttt ttttatgcct tccggttctg atctgtttac
     6421 ggatccggag tgaaggtcct tcttctcttc cgttcgcacc ggctcctgat ggccgatagt
     6481 ccccgaacag aagttcggtt ccgcagcgta cttttagccg aaagcttgtt tacctttatg
     6541 caaaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngagc agatgaacgc
     6661 gaccaatcac ttcggaacgg cattcggatt agggatgagt tcgttgcgtg tggatcataa
     6721 cgcgatggcc gccaccgaca cggatggcgt cggaacggtg ttggtcccga tacccgacag
     6781 cctcgactac aagcagaatc gctacgtcat gaattcgatc gacgcggcgt tcgagtttag
     6841 gttccgcggt aatcccaaca agaatcacaa gcatttcaaa ttcagtcttg gtttcagggc
     6901 cggttatgtg atacaaagtc acctgaagta caaagacgag gacgtgaaat acaaactgta
     6961 ctcgatcgag gacatccagc gctggcgcta tggcgtgacc ggcaggatcg gtctgggcca
     7021 cctcgcgctc gatgcctatt acagcctggc gcccctgtac aaagaaggga aaggtccggc
     7081 cgacatgatg ccgtattcca tcggcattgc ctggacattc tgatcgttca gatgcttaac
     7141 agcaggaatt cgcagaggaa tcctacgagg aagccggcgt agatttgcat gggcgtatgc
     7201 gcgcccaaac tgagacgggc tgtgccgagc aaaccggcga ccagcaggca gaacaggata
     7261 ggaatccgaa catccaccag gagaaaggtg gacaaaccaa aaaatgttcc gatgatgccg
     7321 ccgatcccga tcatgtggat gctgatcttc catttgaaat tgatgatggc cgcgaaggtc
     7381 acggctaccg cggcgcccag tatcaggagc ggaaacacct gggggagcat caattttcgc
     7441 atcaggtagt acgcgatcaa caacagggcg gcgttcgtca gaaacgggat cacacgctct
     7501 tcccgctttt ccatttcaaa ggaccggatc cagccccgcc ggctcaggat gtaggaaaca
     7561 atggtcggta gcacgagcgt gttgagcaac acaatcatgt aaagcgcgtc gcgctcagga
     7621 ggagaaaccg tataggaaaa gtaggagctg tggtggaaca aaaaccagat cgcgtaggtc
     7681 ggcatcagca ccgggtggag caggatggac aggaattgcg cgaacgtacg gctcatagct
     7741 ccttgcgtaa gcgggcaacg ggtatgtaga gcatctcctg gtatttcgct accgtacgac
     7801 gtg
//
LOCUS       DAOTON010000019         7735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_8360_length_7735_cov_4.983203, whole genome shotgun sequence.
ACCESSION   DAOTON010000019 DAOTON010000000
VERSION     DAOTON010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8360_length_7735_cov_4.983203"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="P5292_00735"
     CDS             complement(<1..777)
                     /locus_tag="P5292_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37678.1"
                     /translation="MKPIRFLTLFFTALALAANFSCSTVSKTPLTEAQLETKLREHIS
                     TLASDAYEGREAGSAGEIKARDYIVKAFKSAGLKPKGDKGFLQPFTFTAGATFGPGTQ
                     LYVNNYSFKVNDDFTPLPYSGNGVITGYVARLGYGIDDNKLGREDYKGKLNIAKKIFV
                     MESGTPDGANPHGKFGEWSDLRKKLDIAIQRGATAVIFINSDTAQADPKADFDHRVSP
                     VSIPVLFAKGKAAELLRDSIVTNCTVGAEVLKVTKDAYNVC"
     gene            complement(886..2430)
                     /gene="lysS"
                     /locus_tag="P5292_00740"
     CDS             complement(886..2430)
                     /gene="lysS"
                     /locus_tag="P5292_00740"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRS37679.1"
                     /translation="MELHEQEQIRRQKLDELQKHGYQAYPADAWPVTVSARQILDGFD
                     ANPDGFKELSYAGRIMSVRDMGKALFAVLQDASGRIQIYVRRDDICPGEDKSDYDFLV
                     KKMLDIGDIIGIKGFVFRTKMGEITIHATAVKLLSKSLRPLPIVKQDADGNVHDAFTD
                     PEQRYRMRYVDLIVNPQVRETFVKRTQLVNSMRSFLNTRGYLEVETPILQPLYGGAAA
                     RPFKTHHNTLDMTLYLRIANELYLKRLIVGGYDGVYEFSKDFRNEGMSRFHNPEFTQM
                     ELYVAYQDYEWMMNLVEEMVEKIALDLHGKTEVQVGEHVINFQRPWKRYTMYGAIAHY
                     TGIDISGMDEAELRKTARSIGIHVDDTMGRGKLIDEMFGEKVEAHLIQPTFITDYPLE
                     MSPLAKQHRSKPGLVERFEAICNGKEICNAFSELNDPLDQRKRFEDQLELGKRGDEEA
                     MQLDEDFLRALEFGMPPTAGLGIGIDRLSMIMTNSHSIQDVLFFPAMRPEKKETPAEG
                     TEAVSS"
     gene            2592..3584
                     /locus_tag="P5292_00745"
     CDS             2592..3584
                     /locus_tag="P5292_00745"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HRS37680.1"
                     /translation="MKDLVQRFPEQLEEALRIGEQASISRPIRPIRNVIITGLGGSGI
                     GGTIVSEIIANECPVPVTVNKDYFLPAFTGPETLVIVSSYSGNTEETLQAMDAALEKG
                     AQISCITSGGSVAERAREHGLDLVLIPGGNPPRSCLGYSLTQLFFILHKKGLIGGAFV
                     AQLRASIDRMRMEEAAIKSEAQEVTDFLFGKLPVIYAVDGYNGVATRFRQQINENSKM
                     LCWHHIIPEMNHNELVGWAEQHEECAVVILRNSTDYYRSQARIEINKEIIAQRTPHIR
                     EIWSRGDSMLERSLYLVHLTDWVSCYLADRKAIDAVEVNVINHLKGSLAKLGER"
     gene            complement(3822..6251)
                     /locus_tag="P5292_00750"
     CDS             complement(3822..6251)
                     /locus_tag="P5292_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS37681.1"
                     /translation="MRTRLLFSFFLLLSCLAFARTNRTVSGILHGSDRQPLAYATVML
                     LNAADSSLVKGAITDEQGAYRFDDLQPGRYRVKATQVGLETTLSAEFEVTEAGTEHPL
                     PTLVMGGKSVQLKEATVSGEKPFLEHRVDKIVVNVENSIVNAGGTALEILQKSPGVTV
                     DNNGNIFLRGKQGVLVMMDGKPTYLSAQELYNMLRNMPADQLAQIEIITNPSAKYDAA
                     GTTGIVNIRMRKNRNYGTNGDLRTSYGQGRYLDYGFGGSVNHRNKLFNAYGSYDYGNG
                     FYFESTEMQRRFRDGNFVSTFKQDNYDKGEYINHNFRGGLDVSLSPKHTIGFLVRGTY
                     NTGENHNRSVTDILNASDIPDSGYVTRNDNNSEWNNLNGNFNYRWAIDSAGQELSIDA
                     DLGRFDNKADFNFYTDHYDYLNRSNDWVERSYNRQPAVIDIRTAKADYVKPIGKKSRF
                     EAGAKISDVETDSDVRFFNIINGADVADTGKTNHFVYEETIRAGYVNGTTEFGKIGLQ
                     LGLRAEQTIAKGTQQVNNSTFTRDYTQLFPTAFLTYSFNDTHQVHVSYGRRIDRPAYQ
                     QLNPFKFYLDPYTYQEGNPLLQPQLTNAYEIGYTFLQRYSINLNFSRTNNAMTQITRQ
                     IDSLRTTYIITENLQTNDNLSVNLNLPFQPFPWWEMNNNLTVYNTRFKGPSSAGEVDV
                     QRTAVSFNTNHAIRLPKEWALEVNGYFNSASVWGTWMVRPFGSMSMGFRKQLLDGKVT
                     LRINVNDPFWTDRIRSTVRMENIDAQFNRFYDSRFVRFHVSWKFGKQTVAGNRQRRTG
                     AEEEQNRIQRR"
     gene            6362..7351
                     /locus_tag="P5292_00755"
     CDS             6362..7351
                     /locus_tag="P5292_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HRS37682.1"
                     /translation="MSKYKRIDLQERIRIETFLGLGYTCTAIAQELGRPTSAITRELD
                     RFDGYYSAIEAQHLAELRRVTQRKTRKLDANPALLAEVRGLLRKRYSPEQISRHLIEE
                     FPDDPTMQISHESIYTFIYMMPRGDLRKELIASLRQKKKKRNKRGLSIERRGKIPDMV
                     SIDERPDYILNRSVPGHWEGDLIVGKRHQSAIGSIVERKTRAVILVKLKAKDAESVRR
                     AFEKEFKSIPRQMKKSMTYDQGHEMAEHKLFSKNTRMKVYFCHPGSPWERGSNENMNM
                     LIRDFYPKGTDFNKITRRELKRVQDLLNERPRKTLNWKSPFDVFENEIMKKCS"
     gene            7544..>7735
                     /locus_tag="P5292_00760"
     CDS             7544..>7735
                     /locus_tag="P5292_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37683.1"
                     /translation="MNQEHVWEDDQIKFLRDRLHLKMIDAFREYQSFYGEIDINKFQE
                     KYKEFKNKYTKSHKFTNEEK"
BASE COUNT         1689 a         2104 c         2171 g         1771 t
ORIGIN      
        1 gcagacgttg taagcgtctt tcgtcacttt gagcacctcg gctccgaccg tgcaattcgt
       61 cacgatgctg tcgcgcaaca gttccgccgc cttgcccttc gcgaacagta cgggaatgct
      121 caccggtgag acgcggtgat cgaagtcggc ttttggatcc gcctgtgctg tgtcgctgtt
      181 gatgaagatc acggccgtgg ccccgcgctg gatcgcgatg tcgagtttct tccgcaagtc
      241 ggaccactcg ccgaacttac cgtgcgggtt cgcgccgtcc ggcgtacccg attccatcac
      301 gaagatcttc ttggcgatgt tgagcttccc tttgtaatct tcacgaccga gtttgttgtc
      361 gtcgatcccg tagcccagcc gggccacata gccggtgatg acgccgttgc cggagtaggg
      421 gagcggcgtg aagtcgtcgt tgaccttgaa gctgtagttg ttcacgtaca actgcgtgcc
      481 cggaccgaag gtcgcgccgg cggtgaaggt gaacggctgc aggaagcctt tatcgccttt
      541 cggtttcagg cccgccgact tgaacgcctt cacgatgtaa tcgcgcgctt tgatttcccc
      601 ggcgctgccg gcttcacgtc cctcgtaggc gtcgctggcg agagtgctga tatgttcccg
      661 cagcttcgtt tccagttgcg cttcggtgag cggcgtcttg gaaacggtgg aacaggaaaa
      721 gttcgccgcc agggcgaggg cggtgaagaa cagggtcagg aaacggatcg gcttcattgc
      781 agcataaaga tacggaatcg acgtgcaagc cttgtcgcag ggagcggctt ccgggaataa
      841 aaaagggcac gctcggtgcc cttttgactt ttttctgatg atgaatcaac tggatacggc
      901 ttcggtgccc tccgccggcg tttctttctt ctccgggcgc atggccggga agaagagcac
      961 gtcctggatc gaatgactgt tcgtcatgat catgctcagg cggtcgatgc caatacccag
     1021 gcctgcagta ggcggcatgc cgaactcgag cgcgcgcagg aagtcttcgt cgagctgcat
     1081 ggcttcttcg tcgccgcgct tgccgagttc gagctggtcc tcgaagcgct tgcgctggtc
     1141 gagcggatcg ttgagctcgg agaaggcgtt gcagatctcc ttgccgttgc agatcgcctc
     1201 gaaacgctcc accagccccg gcttggagcg gtgctgcttc gcgagcggac tcatctccag
     1261 cggatagtcg gtgatgaagg tcggctggat caggtgcgct tcaactttct ctccgaacat
     1321 ctcgtcaatc agcttaccgc ggcccatcgt atcgtccaca tgaatgccga tgctccgcgc
     1381 cgtctttcgc agttcggctt cgtccatccc ggagatgtcg atgccggtat agtgcgcgat
     1441 cgcgccgtac atcgtataac gcttccaggg ccgttggaag ttgatcacgt gttcacccac
     1501 ctgcacctcg gtcttgccgt gtaggtcgag tgcgatcttc tcgaccatct cttccaccag
     1561 gttcatcatc cattcgtagt cctggtaagc gacgtagagt tccatctggg tgaactccgg
     1621 gttgtggaac cggctcatgc cttcgttccg gaagtccttc gagaactcgt acaccccgtc
     1681 gtagccgccc acgatgaggc gcttgaggta caactcgttc gcgatgcgca ggtagagcgt
     1741 catgtcgagc gtgttgtggt gcgtcttgaa cggacgcgcc gccgcgccgc cgtagagcgg
     1801 ttgcaggatc ggcgtctcca cctcgaggta gccgcgggtg ttcaggaacg aacgcatcga
     1861 gttgaccagt tgcgtacgct tcacaaatgt ctcgcgcacc tgcgggttca cgatcaggtc
     1921 cacgtaccgc atccggtagc gctgctccgg atcggtaaag gcgtcgtgca cattgccgtc
     1981 ggcgtcctgc ttcacgatcg gcaacggacg cagtgacttg gaaagcaact tgacggcggt
     2041 ggcgtggatg gtgatctcgc ccatcttcgt gcggaatacg aagcccttga tcccgatgat
     2101 gtcgccgatg tcgagcatct tcttcaccag gaaatcgtaa tccgatttat cctcgcccgg
     2161 acagatgtcg tcgcggcgga cgtagatctg gatgcgtccg gatgcgtcct gcagcactgc
     2221 gaacagggcc ttgcccatgt cgcgtacgct catgatccgg ccggcatagc tcagctcctt
     2281 gaaaccgtcg gggttggcgt cgaagccgtc gaggatctgg cgggcgctga cggtgaccgg
     2341 ccaggcgtcg gccggatagg cctggtagcc gtgtttttga agttcgtcca acttctggcg
     2401 gcggatctgt tcctgctcgt gcaattccat gctgcgaaat tttcgcaaag atagcggttc
     2461 aggccggatg gctcaaaacg ttaatcgata cagaatcaat aaattgcaac aagtccacgc
     2521 aacgcgcgtt cggcccggaa tcggtaaccc cctacctttg cggccgtata cgccggactc
     2581 cattaatagt catgaaagat ctcgtacagc gattccccga gcagttggaa gaagccctgc
     2641 gcattggcga acaagccagc atttcccgtc ccattcgccc catccgcaat gtcatcatca
     2701 ccggactggg cggttcgggt atcggtggca ccatcgtgtc cgagattatc gcgaacgaat
     2761 gtccggtacc cgtcaccgtc aacaaggatt atttcctgcc ggctttcacc ggaccggaga
     2821 cgttggtgat cgtgtcgtcg tattcgggta ataccgaaga gaccctgcag gccatggacg
     2881 ccgcgctgga gaaaggcgcg cagatcagtt gcattacctc cggcggcagc gtggccgaac
     2941 gcgcacgcga gcacgggctc gacctcgtcc tcatccccgg cggcaacccg ccacgttcct
     3001 gtctcggtta ttcgctcacg cagctctttt tcatcctgca taagaaaggg ctcatcggcg
     3061 gcgcgttcgt cgcgcagctc cgggcttcga tcgaccggat gcgtatggaa gaagcggcca
     3121 tcaagtccga ggcgcaggaa gtaaccgatt ttcttttcgg caaactgccg gtgatatacg
     3181 cggtcgacgg ttacaacggc gtggccacgc gcttccggca gcagatcaac gagaactcca
     3241 agatgctctg ctggcaccac atcatccccg agatgaacca caacgaactg gtgggctggg
     3301 ccgaacaaca cgaagagtgt gccgtcgtga tcctgcgcaa cagcaccgac tactaccgtt
     3361 ctcaggcgcg catcgagatc aacaaggaga tcatcgcgca acgcaccccg cacatccgcg
     3421 aaatctggtc gcggggcgac tccatgctgg agcgttcgct ctacctggtg cacctcaccg
     3481 actgggtctc ctgctacctc gccgaccgca aagccatcga cgccgtcgaa gtgaacgtca
     3541 tcaaccacct caaaggctcc ctcgcgaagc tgggggagcg atgatagttc cgccagtgcc
     3601 ttattcatga tggaacgcgg atcccgcgga taaggcggat cctcgcggat aactgtccgc
     3661 gcaaatccgc aacatccgcg ggatccgcgt gccatcaatt cttgatcact atcgggatgc
     3721 ccggactttt aaccacggtg gacacagagg aatgcacgga gggacacgga gatcgaacgc
     3781 tgatgtacac taattagctt catcacgcaa ttcaatcttg cctatcgccg ctggatgcgg
     3841 ttctgttctt cttcggctcc ggtgcggcgt tggcggttgc cggctacggt ttgcttgccg
     3901 aatttccagg atacgtggaa gcgtacgaaa cggctgtcgt aaaagcggtt gaactgcgcg
     3961 tcgatgttct ccatccgcac cgtcgaccgg atacggtccg tccagaacgg atcattcacg
     4021 ttgatgcgca gggtcacctt tccgtcgagt aactgcttcc gaaagcccat cgacatgcta
     4081 ccgaacggcc gcaccatcca ggtgccccag accgatgcgc tgttgaaata gccgttcacc
     4141 tccagcgccc actccttcgg taaccggatc gcatggttgg tattgaagga taccgccgta
     4201 cgctgtacgt ccacttcgcc cgccgaactg gggcccttga agcgggtgtt gtacaccgtc
     4261 aggttgttgt tcatctccca ccaggggaac ggctggaaag gcaggttgag gttcacactc
     4321 aggttgtcgt tcgtctgcag gttttccgtg atgatgtagg tcgtccgcaa cgaatcgatc
     4381 tgccgcgtga tctgcgtcat cgcgttgttg gtccgactga aattcaggtt gatcgaatag
     4441 cgttgcagga aggtgtagcc gatctcatac gcgttggtca gttgcggctg caggagcgga
     4501 ttgccttcct ggtaggtata aggatccagg tagaacttga acggattgag ctgctgatac
     4561 gccggccggt cgatgcggcg cccatacgaa acgtgtacct ggtgcgtgtc gttgaagcta
     4621 taagtaagga aagcggtcgg gaagagctgc gtatagtcgc gggtgaaggt cgaattgttc
     4681 acctgttggg tgcccttcgc gatcgtctgt tccgcgcgca ggcccagttg caggccgatc
     4741 tttccgaact ccgtcgtgcc gttgacataa cccgcccgga tcgtctcttc gtacacgaag
     4801 tgattcgtct tgccggtgtc cgccacgtcc gcgccgttga tgatgttgaa gaaccgcacg
     4861 tcgctgtcgg tctccacatc gctgatcttg gccccggcct cgaagcgcga ctttttcccg
     4921 atcggtttca catagtcggc tttcgccgtc cgaatgtcga tcacggccgg ttgccggttg
     4981 taggaccgct cgacccagtc gttgctgcgg ttcaggtaat cgtaatggtc ggtgtagaaa
     5041 ttgaaatcgg ccttgttgtc gaagcggccc agatcggcgt caatcgagag ttcctgcccg
     5101 gcgctatcga tcgcccaacg gtagttgaaa tttccgttga ggttgttcca ctcgctgttg
     5161 ttatcgttcc gcgtcacgta acccgaatcg ggaatgtccg aagcattgag aatatcggtc
     5221 acgctccggt tgtggttctc gccggtattg taggtcccac ggaccaggaa accgatcgtg
     5281 tgcttaggcg aaagactcac atccagtccg ccgcggaagt tgtggttgat gtactcacct
     5341 ttgtcgtagt tgtcctgctt gaaagtcgag acaaagttcc cgtcacgaaa ccgccgctgc
     5401 atctccgtgc tctcgaaata gaaaccgttg ccataatcgt aactgccgta ggcgttgaac
     5461 agcttgttcc ggtgattgac gctgccgccg aatccgtagt cgaggtagcg gccctgtccg
     5521 tagctggtgc gcaggtcgcc gttggtgcca tagttgcggt tcttgcgcat cctgatgttc
     5581 acgatacccg tcgtgccggc agcgtcatac ttggcggagg ggttcgtgat gatctcgatc
     5641 tgcgcaagct gatccgccgg catgttgcgc agcatgttgt aaagttcctg ggcgctcagg
     5701 taggtcggtt tgccgtccat catcacgagt acaccctgct tgccgcgcag gaagatgttg
     5761 ccgttgttat ccaccgtcac gccgggcgac ttctggagga tttcgagggc cgtgccgccg
     5821 gcattcacga tgctgttttc cacgttgacg acgatcttat cgacccggtg ctccagaaag
     5881 ggtttctcac ccgatacggt cgcctctttc agttgcaccg acttaccacc catgaccagg
     5941 gtgggcaggg gatgctccgt gccagcctcc gtcacttcga actccgcgga gagcgttgtt
     6001 tccaatccca cctgcgtcgc ttttacacgg taacggccgg gctgcaggtc gtcgaagcgg
     6061 taagcgcctt gctcatcggt gatcgcgcct ttgaccaggc tgctgtctgc cgcattcagc
     6121 agcatcacgg ttgcatacgc gagtggctga cggtcggaac cgtgcaggat accggatacg
     6181 gtacgattgg tccgggcgaa tgccagacag gacagaagaa gaaaaaagga gaacaacagg
     6241 cgggtacgca tgggaggggg aatggtgtta ccaaacctac gtctctcagc aggtcatgcc
     6301 ggttaatgat agcttacttc caagttccta acgcaacata aaactattca tatgttgcaa
     6361 catgagcaag tataagcgaa ttgatttaca ggagcgaatc cggatcgaga cgttccttgg
     6421 gttagggtat acctgtactg cgatagcgca ggaattgggg cgccccacta gtgccattac
     6481 gcgagaattg gaccgatttg atggctacta ctctgcaatc gaggctcagc atttggccga
     6541 actcagacgt gtcactcaac gaaaaacaag gaagttggat gccaaccctg ctttactggc
     6601 ggaagttcgc ggattactca ggaagcgtta ctctcctgaa caaatttcaa gacatttgat
     6661 agaggaattc ccagatgatc caacaatgca gatatcccac gaatcgatct atactttcat
     6721 ctatatgatg ccccgaggcg atttgcgcaa ggaattgatt gcgagtttgc gtcagaagaa
     6781 gaaaaaacga aacaagagag ggttgtccat tgagcgcagg ggtaagatcc cggacatggt
     6841 gagcatcgac gagcgtcctg attacattct caatcggtct gtacctgggc attgggaagg
     6901 agacctgatt gttggaaaac ggcatcagtc cgccattggc tccatcgttg aacgaaaaac
     6961 ccgggctgtg atactggtaa aactgaaggc gaaagacgca gaaagtgtcc gaagagcttt
     7021 cgagaaagag ttcaaatcaa ttcccaggca aatgaagaag tccatgacct atgatcaggg
     7081 acacgaaatg gccgagcaca agctgttctc aaagaatacg cgcatgaaag tgtacttctg
     7141 tcacccaggc tctccctggg agcgcggatc caacgagaac atgaatatgc tcatccggga
     7201 tttttatccg aaaggaaccg acttcaataa aatcacgcgg cgggaattga aacgagttca
     7261 ggacctgttg aacgaaaggc cacgcaaaac gctcaactgg aaatctccct ttgatgtctt
     7321 cgaaaacgaa atcatgaaaa aatgcagtta agacaaatgt attattttta acaaaatgtt
     7381 gcgttagaag ttagaatgta ccttatgcaa aaattgaaaa tatataatat tttgaagata
     7441 ggtaaagatt tatgaaataa attaatgcca cgtgggactg actcaattct aaaatatgca
     7501 ctttatgcta gtcaaggaat aacaatctca aatctataaa tatatgaacc aagaacatgt
     7561 ctgggaagac gatcaaataa aattcttgcg tgatagatta catttaaaaa tgattgatgc
     7621 atttagagaa tatcaatctt tttatgggga aatagacata aacaaatttc aagagaaata
     7681 taaagaattt aaaaacaaat acactaaaag ccataagttt acgaatgaag agaaa
//
LOCUS       DAOTON010000020         7637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_8468_length_7637_cov_3.639805, whole genome shotgun sequence.
ACCESSION   DAOTON010000020 DAOTON010000000
VERSION     DAOTON010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8468_length_7637_cov_3.639805"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..7632)
                     /locus_tag="P5292_00765"
     CDS             complement(<1..7632)
                     /locus_tag="P5292_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRS37684.1"
                     /translation="MAGSPLQQGVWQFVAFTFNGNTKQAELYIDGARVGTVNSPGVSL
                     GADGSSVVLGADVAPFMNNFFQGAIDEVMIYGRVLTSEEILRISSATRDPGEVCNGID
                     DDCNGLTDEGDTTPPTITNGNNLLQSYRFNSGLLEDVHGSYGRMTSNGGSVGGDLYSF
                     GQNQGLSLRGGFDPANYSIELQFTPFNTAGYRKLIDFKNLTQDAGLYITATGFDFYTV
                     GGGSEPFLQNQLNRLVITRDGSTKVVSIFVNNVPAFDFIDAGDAAVFSSPDTVAHFFI
                     DDNGGVEATDGTVDRIRIFGSPLNPTQVDALYNPNAATNFQEYLLSGQCSTASTSFPF
                     TAADNCGNANVTYASIDGPPFYVGAVNEVYTIAVDDAGNYSYATFLYTIFPVDTWYAD
                     NDEDGFGDPNNDTLACSAPIGYVANNTDCNDADPGSQSLRPVVVHSLPAGIPNCIAVL
                     DTLSVFEYQSQAFANQVLGFSSEFTDADWSASRILGAPDVYPSYGDNVNAWAPFGQDA
                     SREFIELGFNNNTPINLIEIYETYTPGAIDTVSVWNPYTSDYQIVYTAAAGAFPEVAN
                     KLQIRFPVTAFPVSRIRIAMNSGFVHDWNELDAVSIGMIVPTSYSAYTWSTGETTPSI
                     TVSTAGQYNVSVANGACVANSGIAIRNQHFLAGPSFDWTICQGASVVLAVGGGDTYSW
                     STGETTQAITVSPTETTVYTVDGTDVYGCSMSDEITVYQYDPIQVASISVIPESAPSA
                     NNGSIEVTGVTGGVAGGYTYSLFFEAALVASNGTGLFTLLAPGDYYLVVNDNAPCEFS
                     TAVTVPPATVCTAAETPTIIQYDPIICPGEDLFLTVNGSALNDNTDWYWYEGSCGGTP
                     IETGANLYIPLATNTTYYVRGEGGCVQTGACASIAITPLLPPNISFNVSATDITCNGA
                     EDGTISILNATGGAPGVDGYTYTVSDGESYFSNHTGLFTGLWSSSYFVLVTDTSVCGL
                     QDTVDYFINEPDPIAMTINLAANSCYGQDVTGFIAVTGGTLPYTGDGAFNLGALAPTD
                     SVLGFLVSDANGCQQVDSVVFSQPSALVLDGVTDSTIVCLEFSTTGELTLTPSGGTAP
                     YTITGDATTGLVDGTYHYTLTDNNGCVLNKTTEVFVTNCILPYYNAPDSGKVNTALGP
                     ELVQLYNDPDSIGPQNNNVVFQLDGFGSVFIDVIANIGQYDALLNLLQTPQYGMTGII
                     DNGDTTLIITGYYPIDNLPLLDSIPTLVNFVRPNYAPVSSAGLINSQGDRAMKSDSAR
                     VLFDLDGSGIKVGVISDSYNKLAGNPAGLDISNKDLPGPGNATNPTPVQVLLDYPYGA
                     GLDEGRAMMQIVHDVAPKAELAFRTGFISAGDMAEGIVELQQAGCDVIVDDVTYITEP
                     YFQDGLVSRAVNAVRDSGVAYLTSAGNFGSVSYEGIFNPINAPSGLTGKVHDFGGGDY
                     LQLLNLKKGSYTLVLQWDDSLYSLKQLPGARNDFDVYLTDVTGTKLFGLNRKNNWGDP
                     IEVLPFTVLSNVQANLMIVRTWSQDTITPMKFKYIVFRGDMTIAEHPGGNSTIIGQAN
                     AEGAMSVGAVRYTQTPVFGTPTPVIETFSSVGGTPVKGVVRNKPDFSAPNGVNTSVNF
                     FSKDIESDGLYNFFGTSCAAPHAAGTAALLMQAREKFYSEHMLPDTVRSILQQTAIDM
                     GDAGFDYASGYGLIDANAAIMTFAEPKPLIDTVVATDPQAVPGVTDFEVTVSGSYFSD
                     SSVVYFDGQPLPTDFVDPTHLLVIVPAFTGNPMLQVYNPPTVPNVNDGGFSNGYYFYN
                     SVKPVITVRADNSVKYYGESLPAFTSTILVDGQPLSSTSLSLADLGLDDLQYQTIATP
                     LSGIGFYQVMPVMVPLDLNDPDDLTIDTSYEFTFEPGILKINKLPITITPRDTTLTYG
                     DKITGIQFKYDYDDSNIDPGFRVDFLDSIQETHQLTMSNALVLVNPRVVTNPRVVTNS
                     DFANLAMVATGQALSNPRVVTNPRVVTNTVVIDTMEIIDLSAESVFNYDNDSSLIILE
                     TPTGGLRSRAMANPRVVTNPRVVTNPRVVTNGVLDADTTALDTDNSDNIAVVLDEDDV
                     NQPDTATQLKSVNLITGTTPGNWFIVPGAFFDDNFEISYGIGNLTIVPAEITVKAKDT
                     TLDCFGTPPFEAEVSGFQYQDEEGVDANSGPSFDLLDDQNQSVATITAGGSYTIVPSD
                     YSFAADSNYVIVYENGTLQLPDPLVLNAIADPIGCFGATTSVTLLATGGNGGYQYGGD
                     AISGLSEGSYQYTVTDLKGCTATASVTIQPAPAKLLISATVTQPNCHGETGSVILDAT
                     GGVGPYTYSNTPTTGLTNGTYVYEVTDANGCTNSIEVEIQAAPSNLVLNAFTTQPACF
                     GDLGSVLLSPTGGVGPYTLGLDPVNDLGPGSYQYTVTDANGCAAVATAVINPAPDPVV
                     LIATPVNPSCFGQTGSVLLSATGGNGTYTYGGDATTGLGSGTYQYTVSDVKGCSGSAS
                     AQIVVPTQISINTTSTPTGCSGNTGTATAAPSGGTPGYQYLWTPGGGTTATIGSLASG
                     IYQVTV"
BASE COUNT         1780 a         2045 c         2161 g         1651 t
ORIGIN      
        1 caccgtaacc tggtagatgc cggatgcaag tgagccgatg gtggcggtcg ttcctccacc
       61 aggcgtccag aggtactggt accccggagt accgccactc ggcgcagcag tagcagtgcc
      121 ggtgttaccg gaacaaccgg tcggggtcga agtagtattg atgctgatct gggtgggcac
      181 gacgatctgc gcactggcgc ttccggagca acctttaaca tcgctgaccg tgtattgata
      241 cgtaccggag ccaagtccgg tcgtagcatc cccgccatag gtgtaagttc cgttaccgcc
      301 ggtagccgat agcagtacgc tgccggtctg accgaagcag gaaggattca cgggcgtggc
      361 aatgagcacg accggatccg gggccgggtt gatgacagct gttgcaaccg cggcacaacc
      421 gttcgcgtcg gtgacggtgt attggtaact acccgggcca agatcgttta ccggatccaa
      481 tccaagcgtg taaggcccta cgcctccggt cggcgataaa agcaccgagc cgagatcgcc
      541 gaagcaagcc ggttgcgtag taaacgcgtt caagacgagg ttgctgggag ctgcctgaat
      601 ttctacttcg atactgttcg tacaaccgtt cgcatccgtt acctcataca cataggttcc
      661 gttggtcaat ccggtcgtag gagtattcga ataggtgtaa ggtcctacac cgccggtcgc
      721 atcgagtatg acacttccgg tctcgccatg acaattgggt tgtgtaactg tagcagagat
      781 caacagctta gccggagcag gctggatcgt cacactggca gttgccgtac aacctttaag
      841 atcagtgacc gtgtattggt aactgccctc ggacaaaccg ctgattgcgt ctccaccata
      901 ttggtatcct ccattgcctc cggtagccag cagcgtaacg ctcgtcgttg cgccaaaaca
      961 accgatcgga tcggcaattg cattcagcac gagcgggtcg ggcaattgaa gcgtaccgtt
     1021 ctcatatacg atcacgtaat tcgaatccgc ggcaaaggag taatcactcg gtacgatggt
     1081 gtacgatcca cccgctgtaa tggtggcaac ggattggttc tggtcatcca gcaaatcgaa
     1141 actcggaccc gaattcgcat ctacgccttc ttcatcctga tattggaagc cagaaacttc
     1201 cgcttcgaat ggaggtgtgc cgaaacaatc cagcgtggtg tccttggctt tgaccgtgat
     1261 ctccgccgga acgatggtca gattaccgat tccataactg atttcgaaat tgtcgtcgaa
     1321 gaacgcgccg ggaacgatga accagttgcc gggcgtggta ccggtgatca ggttcaccga
     1381 cttcaactgc gtggcggtgt ccggttggtt gacgtcatct tcatcaagga caacggcgat
     1441 gttatcgctg ttgtcagtat ccagcgcggt cgtatcagcg tcgaggaccc cgttggtaac
     1501 gacgcgtgga ttggtaacaa cgcgcggatt cgtgaccaca cgcggattag ccatggcgcg
     1561 ggaacgcaga ccaccggtcg gtgtttccag gatgataagc gaggaatcgt tatcgtaatt
     1621 gaacaccgat tcggccgaaa ggtcaatgat ttccatcgta tcgatcacga cggtgttggt
     1681 caccacacgc ggattggtta ccacacgcgg gttagataat gcctgaccgg ttgctaccat
     1741 ggctaggttc gcgaagtccg agttcgtcac cacgcgcggg ttggtcacca cacgcggatt
     1801 gaccaacacc agcgcgttcg acatcgtcaa ctggtgcgtc tcctgaatgg aatcaaggaa
     1861 gtcgacgcgg aagccgggat cgatgttcga atcatcgtag tcatacttaa actgaatgcc
     1921 cgtgatctta tcgccgtacg tcagggtcgt atcgcgcggt gtgatcgtaa tcggaagctt
     1981 gttgatcttc agaatacccg gctcaaacgt gaactcatag ctggtatcga tggtgagatc
     2041 atccggatca ttcagatcca gcggcaccat gaccggcatg acctgataga aaccgattcc
     2101 gctcagcggc gtcgcaatgg tctgatattg cagatcgtcg aggccaagat cggccagcga
     2161 gaggctggtc gaagacagcg gctgtccatc caccaggatc gtcgaggtga acgcaggcag
     2221 gctctcgccg tagtacttga cggaattgtc ggcacgtacg gtaatgaccg gcttcaccga
     2281 gttgtagaag tagtagccgt tcgagaaacc accgtcgttg acattcggaa ccgtcggcgg
     2341 attgtagacc tgcaacatgg ggttacccgt gaaagcagga acaatgacca gcaggtgggt
     2401 cggatcgacg aaatcggtcg gcaaaggctg accgtcgaaa tacacgacgg aagaatcgga
     2461 gaagtaacta cccgaaacag taacctcgaa gtcggttacc cccggcaccg cttgcggatc
     2521 agtcgcgacc acggtgtcga tcaacggctt cggctcggca aaggtcatga tcgccgcgtt
     2581 cgcgtcgatc aaaccataac ccgaagcgta gtcgaatccg gcatcgccca tatcgatcgc
     2641 cgtctgctgc aggatgctgc gaacggtatc cgggagcatg tgctcgctat agaacttctc
     2701 acgcgcctgc atcaggagcg cggctgttcc ggcagcgtgg ggcgctgcac aggaagtgcc
     2761 gaagaagttg tacaggccgt cactttcaat atccttcgag aagaagttca cggaagtgtt
     2821 cacgccattc ggcgcggaga aatcaggctt gttgcggacc acacctttca ccggggtacc
     2881 accgacggac gagaaggttt cgatcaccgg ggtcggcgta ccgaagaccg gcgtctgcgt
     2941 gtagcgcacg gcaccgacag acatcgcgcc ttcggcgttg gcttgtccga tgatggtcga
     3001 atttccgcca gggtgttccg cgatggtcat atctccgcgg aagacgatgt acttgaactt
     3061 catcggcgtg atcgtatcct gggaccaggt acgaacgatc atcaggttcg cctgaacatt
     3121 cgacaacacc gtgaacggca atacctcgat cgggtcaccc cagttgttct tccggttcag
     3181 accgaacagc ttcgtgccgg tcacgtcggt caggtaaacg tcgaagtcgt tgcgtgcgcc
     3241 gggtaactgc ttcagtgaat aaagcgaatc gtcccactga agtaccagcg tgtatgagcc
     3301 tttcttcagg ttcagcagtt ggaggtaatc accgccacca aagtcgtgca cctttccggt
     3361 gaggccggaa ggcgcgttga tcggattgaa gattccctca taggatacac tgccaaagtt
     3421 tccggcggaa gtcaggtaag cgactccgga atcacgtacc gcgttgaccg cgcgtgacac
     3481 gaggccgtcc tggaagtagg gttccgtgat ataggttacg tcatcgacga tgacatcaca
     3541 tccggcctgc tgcaattcca caataccttc agccatatcc cccgcgctga tgaaaccggt
     3601 gcggaaggcc agctcggctt tcggcgccac atcatgtacg atctgcatca tcgcgcgacc
     3661 ttcatcaaga ccggctccat acggataatc caatagcacc tgtaccggag tagggttagt
     3721 tgcattgccg ggtccgggaa gatccttgtt gctgatatca agaccggccg gattacccgc
     3781 aagcttgttg tagctgtcgg agataacacc cactttgatg ccggaaccat ccaggtcgaa
     3841 gagtacgcgt gccgaatcgg acttcatcgc gcggtctccc tgtgaattga tcaggccggc
     3901 actgcttacg ggcgcgtagt taggacggac aaagttgaca agtgtcggaa tggaatccag
     3961 cagtggcagg ttgtcgatcg gataataacc ggtaatgatc agcgtggtat cgccgttatc
     4021 gatgataccg gtcatgccgt actgcggggt ttgcagcagg ttcagcaacg cgtcgtactg
     4081 accgatgttc gcgatcacgt cgatgaagac ggatccgaaa ccatccaact ggaatacgac
     4141 gttgttgttt tgcggtccga tcgaatccgg atcgttgtac aactgcacga gctctggccc
     4201 gagcgcggtg ttcaccttac cggaatccgg cgcgttgtaa tagggcagga tacaattggt
     4261 gacgaacacc tcggtggtct tattcagcac acaaccgttg ttatcggtca gtgtatagtg
     4321 gtaggtcccg tcaacaagac cggtggttgc atctccggtg atcgtataag gagccgttcc
     4381 gccagacggt gtcagggtga gttcgcccgt ggtcgagaac tccagacaaa caatggtgga
     4441 atcggtcaca ccgtcgagca ccaatgccga gggctgactg aatactaccg aatcgacctg
     4501 ctgacaaccg ttcgcgtccg acaccagaaa acccagaacc gaatccgtcg gcgccaaagc
     4561 acccaggttg aatgctccgt caccggtata cggcagggtg ccgccagtga ccgcgatgaa
     4621 tcccgttaca tcctggccat agcaactgtt cgccgccagg ttgatggtca tagcaatcgg
     4681 atccggttcg ttgatgaaat aatcgaccgt atcctgcaag ccacaaacag aggtgtctgt
     4741 aaccagcacg aagtagctgc tggaccacaa gccggtaaac aagcccgtgt ggttcgagaa
     4801 gtaggattca ccatcggaaa ccgtataggt gtaaccatcc actcccggcg ctccaccggt
     4861 tgcattgaga attgaaatgg taccgtcttc tgccccgtta caggtgatgt ccgtggcact
     4921 gacgttgaag ctgatgttgg gaggcaacag cggtgttatc gcaatggacg cacaagcacc
     4981 cgtctgtaca catccgcctt caccacgtac ataataggtg gtattggtgg ctaatggaat
     5041 atacaggttc gctcctgttt cgataggcgt tccgccgcag cttccttcat accagtacca
     5101 gtctgtgtta tcgttcaggg ccgagccgtt gaccgtcagg aacaggtctt ctccggggca
     5161 aatgattgga tcatattgga tgatcgtcgg tgtctcggct gccgtacaaa cggttgcagg
     5221 aggtacggtg accgcggttg aaaattcgca aggggcgttg tcgttgacaa caagataata
     5281 gtcccctgga gccaaaagcg taaataaacc ggtgccgttc gaagcgacca atgccgcttc
     5341 gaagaacagc gagtacgtat atccgccggc caccccgccg gttacaccgg tgacttcgat
     5401 cgatccgttg tttgccgatg gtgcggactc gggaataacc gatatggatg cgacctggat
     5461 cggatcgtac tgataaacgg tgatctcatc gctcatcgaa catccgtata cgtccgttcc
     5521 gtcgacggta taaacagtag tctctgtcgg tgagactgtg atggcttggg tcgtttcacc
     5581 ggtgctccag ctataggtat cacctccgcc taccgctaac acaacggatg caccctggca
     5641 tattgtccag tcgaaagagg gtccggccag aaaatgttga ttacggatcg caataccgct
     5701 attggccaca catgccccgt tggcgacgct cacattatac tgtcctgctg tgctgacagt
     5761 gatggaaggg gtcgtttcac cagtgctcca ggtgtaggcg gaatagctgg tggggacgat
     5821 cataccaatg gaaacggcat ccagctcgtt ccaatcgtgt acgaagcccg aattcatagc
     5881 gatccggatc cgtgaaaccg ggaaagcggt gacagggaat cgtatctgca gcttgttagc
     5941 tacctccggg aatgcgcctg cagccgccgt atacacgatc tgatagtccg aggtatacgg
     6001 attccaaacc gagaccgtat caatggcgcc cggcgtatag gtttcgtaga tctcgatcag
     6061 gttgatgggc gtgttgttgt taaagcccaa ttcgatgaac tcgcgtgaag catcctggcc
     6121 gaagggcgcc caggcgttca cattgtctcc ataggaagga taaacatccg gagcaccgag
     6181 gatgcgcgaa gcggaccagt cagcatcggt aaactcgctg ctgaagccta atacctggtt
     6241 ggcaaatgcc tgagactggt actcaaacac ggaaagcgta tccaaaacgg cgatgcagtt
     6301 cgggataccg gcaggaagac tgtgcaccac aaccgggcgc agggattgcg aaccgggatc
     6361 agcatcgtta caatcggtgt tattggctac ataaccgatc ggtgcactac aggccagggt
     6421 atcgttattg ggatcgccga atccgtcttc gtcattgtcg gcataccagg tatcgaccgg
     6481 aaagatcgta tagaggaagg tggcatacga gtaattcccc gcatcgtcga ccgcgatggt
     6541 atagacttcg ttgacggctc ctacatagaa tggaggacca tcaattgaag cataggtaac
     6601 gttcgcgttt ccacaattgt cagcggcagt gaaggggaag gacgtggagg ccgttgaaca
     6661 ctgacccgat agcaggtatt cctggaagtt ggtcgccgca ttcggattgt ataaggcgtc
     6721 gacctgggtc gggttaagcg gacttccaaa gatgcgaatg cgatccaccg taccatcggt
     6781 agcttccact ccgccgttat cgtcgataaa gaaatgcgct acggtatccg gagaggagaa
     6841 cacagccgcg tcaccggcat cgatgaagtc gaacgctgga acgttgttga caaagatgct
     6901 caccaccttc gtcgaaccgt cgcgcgtgat gaccagtcga ttcaactgat tctgcaggaa
     6961 gggctccgaa ccgccgccga ccgtatagaa atcgaatccg gtagcggtaa tgtataagcc
     7021 tgcatcctgc gtaaggttct tgaagtcgat cagtttacgg tagccggccg tgttgaaggg
     7081 agtaaactgc agttcgatcg aatagttagc cggatcaaaa ccgccgcgca atgacagtcc
     7141 ctggttctgt ccgaaggaat aaagatcacc accaacagaa cctccattag acgtcatgcg
     7201 cccgtagcta ccatgtacat cctcaagcaa tccggaattg aaccggtagg attgcagcaa
     7261 attgtttccg ttggtaatcg tcggtggagt cgtatcgccc tcgtccgtaa gtccgttgca
     7321 atcgtcgtcg atgccgttgc agacttcacc cgggtcgcgg gtcgcggaac tgatccgcag
     7381 gatctcttcc gatgtcaata cgcgaccata gatcatgact tcatcgatgg cgccctggaa
     7441 gaaattgttc atgaagggag ccacatcggc acccaacacc accgaactgc catcggcgcc
     7501 aaggctgacg cccggagaat tgaccgtgcc tactcgcgcg ccgtcgatgt acagttcagc
     7561 ctgcttggta tttccattga aggtgaaggc gacgaactgc cacacgcctt gttgcagagg
     7621 agaccccgcc accgtgt
//
LOCUS       DAOTON010000021         7603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_8518_length_7603_cov_3.350291, whole genome shotgun sequence.
ACCESSION   DAOTON010000021 DAOTON010000000
VERSION     DAOTON010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8518_length_7603_cov_3.350291"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="P5292_00770"
     CDS             <1..287
                     /locus_tag="P5292_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRS37685.1"
                     /translation="LVAWHPKSSTENERTTVVIENGAKTQVLLRQIAGALARRIVCYP
                     QDGWMVKQGEEMGFIKFGSRVDLFLPLDAKIKVQLDQKTRGGETVIAELA"
     gene            complement(320..736)
                     /locus_tag="P5292_00775"
     CDS             complement(320..736)
                     /locus_tag="P5292_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37686.1"
                     /translation="MKRILLPGLLCCLIVFGLSSCEKTDDGDISGNDREKFLGDWLGQ
                     STGSVQGSINFNMTITASNSAPDQVLFENFDGYGSGTFIPATVSGSDFSFIRTIISGD
                     TIEGSGRYNSNNTLSFSFTVRDGQTVDTRSGTARRP"
     gene            complement(842..2524)
                     /gene="hutU"
                     /locus_tag="P5292_00780"
     CDS             complement(842..2524)
                     /gene="hutU"
                     /locus_tag="P5292_00780"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRS37687.1"
                     /translation="MSKTLGTEKLDLTKTKTPHGTLLSCKGWIQEAALRMLLNNLDPE
                     VAERPEDLVVYGGRGKAARNVESFELIVKALKDLRDDETLLVQSGKPVGILPTHRDAP
                     RVLISNSQLVPKWATWDVFDELEKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFNAVA
                     EKHFGGSLKGRLCVTAGLGGMGGAQPLAITMAEGVCLAAEVEEWRAQKRVETRYLDII
                     EHDLDKAIDLALEFKAKGENKSIGVICTAVHMLQRLKQRGIVPDVLTDQTSAHDPLVG
                     YLPERLTIAEANVLRESNPKQYIDLAYDAMAEHVRLMLYMQQQGAITFDYGNNLRARA
                     QERGVQNAFDFPGFVPAYIRPLFCEGKGPFRWAALSGDPQDIAETDKVILELFPENTS
                     LHRWIRMAQERIAFQGLPARICWLGMGERARAGLAFNELVRTGKVKGPIVIGRDHLDT
                     GSVASPNRETEAMLDGSDAIADWPLLNALINTAGGASWVSLHHGGGVGMGYSIHAGMV
                     IVADGTEEAKRRLERVLHNDPAMGVIRHADAGYDIALDTARKFRLDLGSRLK"
     gene            complement(2535..2705)
                     /locus_tag="P5292_00785"
     CDS             complement(2535..2705)
                     /locus_tag="P5292_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HRS37688.1"
                     /translation="MKFTDKFDPEDYYLSPEGYVVFTEKYHLKRGYCCQSGCRHCPWR
                     KKDDTKKRKYPS"
     gene            2810..3742
                     /locus_tag="P5292_00790"
     CDS             2810..3742
                     /locus_tag="P5292_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRS37689.1"
                     /translation="MPLALPEQRIIQTVSLPNKEIGLFVQRDDLSDPWLGGNKVYKLR
                     ENLMAFRRSGKKALLTFGGAWSNHLVATAELCRRAGIPVVGIVRGEENRNPRLDFLRS
                     AGMRLVFSEREQYRRKEDEASQTFYCQAAARELDCSPSDFQVVPEGGSNEAGFQGSKR
                     ILDPAISSTFTHVVVACGTGTTLAGLTASLLPHQQAIGIAVVHDLKNIRERLRTFGVK
                     DERYILLDDFVFGGYAKKNAELLEFCTQFGVDTGIPIEPVYTGKAFFAVIRMINENYF
                     PKGSKVLIIHTGGLYPCDAAFEICTSSKLAVSNS"
     gene            3834..>4725
                     /locus_tag="P5292_00795"
     CDS             3834..>4725
                     /locus_tag="P5292_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRS37690.1"
                     /translation="MLSAFSAGASDAVKLTRTEYISLYKDDAIREMYRSGVPASITLA
                     QACLESSDGNSPLAKVANNHFGIKCSNWDGPGYYQDDDKPNECFRKYNSVFESFDDHS
                     NFLKTRPRYSFLFELNRTDYKGWAHGLKKAGYATDPTYANRLIKIIEEHNLHVYDLAE
                     NPENRPIASTNTIVPVEARPLPPVENTVREQRVFVPSVEPVDMFSNRRIERNNGVEFI
                     RARKGDTFESLSRELSYGYWQLPKYNELPENTPLFEGQIIYIKPKKRDADTRFYVVKP
                     GDTVHSVSQELGMKTRFLCKY"
     assembly_gap    4726..4735
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4789..6249)
                     /locus_tag="P5292_00800"
     CDS             complement(4789..6249)
                     /locus_tag="P5292_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37691.1"
                     /translation="MIGAFPGFGREIKWSPTLPENPKFPYLKIIGGDDEGFFVLRSNI
                     SLDTDRDRAGLRSRKYFLQYFSMEMALRWEGELNAQSDDTRFADIKVVNGRVLVVSYG
                     QDKSTRQFRFYAQYLGTGAAYIGTPVLIDTVAVSRLDEDNKPGILLNRDESQFVFTYR
                     KVPSDGSRQSFEAVLLDTALSLLYRKEIPVDTEQPRFSPLRYVLTERGNFYILGIEFL
                     TDKKVKAPGESYYRLLGYQHSGDRLVNQEIKVQDRFLTDVSLAADNRNNRIVVAGFYS
                     DKTNYTTSGVFYYGLEEDALATTRVLTAPFSQEFVRKTSGDRRDRGSGELVNYSIDRI
                     ILRNDGGAVIVAESFYQTTRTYWDFYMQASVTHYYYHFGNILLISMDPAGSISWSNVV
                     QKDQNSIDDSGYYSSYCSVVSNGMQYSLFNKYIDDDSSVLLSIVDGKGAQRTETLFNE
                     VERVAIIPRSGKQVDEETLLLPAYRQNKLYFVRITF"
     gene            6412..7236
                     /locus_tag="P5292_00805"
     CDS             6412..7236
                     /locus_tag="P5292_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37692.1"
                     /translation="MFFLLAFSAFATQTTQDRKLPFEKESEIAASNGGLSVQFILDVP
                     DCDSLCIGQFSCFVQGGVPPFTYSWSTGSIDSFTTGVCAQSQISLYVEDSVGDWVNTV
                     YFVPQPLRFLVTTTPASCATCCDGSGLGTVSTSCSLPVTYLWNPGMVTLPTINNMCGN
                     ITYSLCVTDVCGCTLCDTLILCGFPAGTSNLIADRPVFQATYASAGQCIMASLLDGPS
                     TAQFDLYDLQGRKILQSTLEKSETRSINAAALASGTYLVVVSDENRLLGNQLLVIP"
     gene            7251..>7603
                     /locus_tag="P5292_00810"
     CDS             7251..>7603
                     /locus_tag="P5292_00810"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromophore lyase CpcT/CpeT"
                     /protein_id="HRS37693.1"
                     /translation="MMRKSGISLLVFQIFLLIQAEAQSPDLTRLTDWMAGSYSSEAQH
                     LRDTANYFDIRLLMAPIWKERSDGHWFYVEQAVADYLDKPYRQRVYRVHEIEPGVFES
                     VIYTLQEPLRFTHHP"
BASE COUNT         1757 a         2103 c         1965 g         1768 t
ORIGIN      
        1 acctggtagc ctggcatccg aagtcgtcca ccgagaacga acgcaccacg gtcgtcatcg
       61 aaaacggagc caagacgcag gtactgcttc gtcagatcgc cggcgctttg gcccgacgca
      121 tcgtttgtta cccgcaggat ggttggatgg taaaacaagg agaagagatg ggcttcatca
      181 agttcggttc ccgcgtcgac cttttcctac cgctcgacgc gaagatcaag gtgcaactgg
      241 atcagaaaac gcgcggcggt gaaaccgtca tcgcggaact tgcctgatcg gtccttcgat
      301 catattacga aatatctttt taaggtcgtc gcgctgttcc gctgcgcgta tcgaccgttt
      361 gtccgtcacg taccgtgaag gagaagctca gcgtgttgtt tgaattatac cgaccgctgc
      421 cttctatggt atcaccggag atgatggtgc ggatgaacga aaaatccgat ccgctgaccg
      481 ttgcaggaat gaaggtgcca ctgccgtagc catcaaaatt ctcgaacaag acctgatccg
      541 gcgcgctgtt cgatgcggta atggtcatgt tgaagttgat tgaaccctgc accgaaccgg
      601 ttgattgtcc cagccagtcg cccaggaatt tttcccggtc gttaccggaa atgtccccgt
      661 catcggtctt ttcacacgaa ctgagtccga atacgatcag gcaacaaagc agtccgggga
      721 gcaggatgcg tttcatggga tgaagataag cagggaacag ccgcgataaa gcaagcacta
      781 tgccggaaaa agaacgggcg aaggtgaaga acccacgccc gtatccgttg cataacttcg
      841 tttatttgag tcggcttccc aggtcgagcc ggaacttccg ggcggtatca agcgcaatat
      901 cgtaccccgc gtccgcatgg cggatcacac ccattgccgg atcattgtgc aagacgcgct
      961 cgaggcgccg cttcgcttct tccgttccgt cagcgacgat gaccataccg gcgtgaatgg
     1021 aatatcccat accgactccg ccgccgtggt ggagcgaaac ccagctcgca ccacctgccg
     1081 tgttgatcaa agcgttcagg agcggccagt cagcgatcgc gtcggaccca tccagcatgg
     1141 cttcggtttc ccggttggga gatgcgactg aacccgtatc gaggtggtca cggccgatca
     1201 cgatgggccc tttgactttg cccgttctta ccagttcatt gaaggcaagc ccggccctgg
     1261 ctctttcacc catgccgagc cagcagatac gcgccggcaa tccctggaag gcgatccgct
     1321 cctgtgccat tcggatccag cgatggaggg aagtgttctc cggaaaaagt tcgagaataa
     1381 ccttatcggt ctctgcgata tcctgaggat cacccgacag ggccgcccaa cggaacggtc
     1441 ctttgccttc gcaaaagagc ggacggatgt aagccggaac gaaaccgggg aaatcaaacg
     1501 cgttctgaac gccgcgctcc tgtgcgcgtg cgcgcaggtt gttaccgtaa tcaaacgtga
     1561 tggcgccttg ctgttgcata tacagcatca ggcgcacgtg ctcggccatg gcgtcgtaag
     1621 ccaggtcgat gtattgcttc gggttcgatt cgcgcagcac attggcctcg gcgatcgtca
     1681 gcctttcggg cagatatcct accaggggat cgtgtgcgga agtctgatcg gtcaatacgt
     1741 ccggtacgat accacgctgc ttcagacgtt gcagcatatg aaccgcggta caaatgacgc
     1801 cgatcgactt attctccccc ttggctttga actccagggc aaggtcaatg gctttgtcga
     1861 ggtcgtgctc gatgatatca aggtagcggg tttccacgcg cttctgtgca cgccactcct
     1921 ccacttcagc ggcaaggcag acgccttccg ccatggtgat ggccaagggt tgcgcaccgc
     1981 ccattccgcc aaggcccgcg gtaacacaga gtcggccctt gagggacccg ccgaagtgtt
     2041 tttcggcaac cgcgttgaag gtctcatacg ttccctgcac gattccctgt gagccgatgt
     2101 agatccagga cccggcggtc atctgaccgt acatgatgag tccttttttc tccagttcgt
     2161 caaagacgtc ccaggtagcc cacttaggaa ccaactgtga attactgatc aggacccgcg
     2221 gcgcgtcacg atgcgtcgga agaataccga ccggctttcc gctttggact agcagcgttt
     2281 catcgtcgcg caagtccttc aacgctttta cgatcagctc gaagctctcc acattccgcg
     2341 ccgcctttcc acggccgccg tacacgacca ggtcttcggg ccgttcggcc acttccggat
     2401 caaggttgtt caacaacatc cgaagtgcgg cttcctggat ccagccttta caactgagga
     2461 gggtgccgtg cggcgttttt gttttcgtca ggtcgagctt ttcggtgcct agggtctttg
     2521 acatagctgc agtgtcagga tgggtatttt cgcttcttgg tgtcgtcctt cttcctccac
     2581 ggacaatggc ggcaaccgct ttgacaacag taaccccgct tgaggtggta tttttcggta
     2641 aagacgacgt agccttccgg agagaggtaa tagtcttcag gatcgaactt gtctgtgaac
     2701 ttcatgggtc cgcaaatctt cggatcacgc cgtcgggaaa tgaacggtaa attttagcaa
     2761 atttaagcaa tccgacagcc ttctccaatg tcggaatatg ctgattatta tgccgttagc
     2821 cttacccgaa cagcggatca tccagactgt atcgcttccg aacaaggaaa tcgggctatt
     2881 tgttcagcgc gacgatctgt cggacccctg gttgggcgga aacaaagttt acaaactccg
     2941 cgaaaacctg atggcctttc gccgttcagg aaaaaaggcc ctgcttacat tcggcggggc
     3001 ctggtcgaac catctggttg ctactgcgga actctgccgc cgcgcaggaa ttcccgttgt
     3061 tgggatcgtc cggggcgaag agaaccggaa tccgcgactc gactttcttc gttcggcggg
     3121 catgcgattg gtcttctccg agcgcgaaca atatcggcgc aaagaagacg aagcatcgca
     3181 gactttctac tgccaggctg ccgcacggga actggactgt tcgccatcgg atttccaggt
     3241 agttcctgaa ggtggctcga atgaagccgg cttccaggga agtaaacgga tcctggatcc
     3301 ggcgatctct tcgaccttca cccatgttgt agtggcctgt ggaaccggaa cgacgcttgc
     3361 cggactcacg gcatccttgc taccgcatca acaggccatc gggatcgcgg tggtgcatga
     3421 tctgaagaat atccgtgaac ggcttcgcac gttcggggta aaggacgagc gctatatcct
     3481 cctcgacgat ttcgtctttg gcggatatgc caaaaaaaat gcggagctgc tcgaattttg
     3541 tacccaattc ggggttgata caggcatccc catcgaaccc gtctataccg gaaaagcatt
     3601 ttttgcggtt atccggatga tcaacgaaaa ctacttccct aaaggcagta aggtgctgat
     3661 catccacacg ggtggcttgt atccgtgcga tgcagcattc gaaatctgca caagtagcaa
     3721 attggctgta tccaattcgt aaaattgccg ttcaaaggga taagaaaata gccctggatt
     3781 ccgttatgtc tttcgtgaaa tgcttccgcc gaattttacc cgccgccctg tcggtgctgt
     3841 ccgccttttc ggcgggtgcc agtgacgcgg taaaactgac ccggaccgag tatatcagcc
     3901 tgtacaagga cgatgcgatc cgggaaatgt atcgttcggg cgttccggcc agcattacac
     3961 ttgcccaggc ctgcctcgaa tcaagtgacg gcaacagtcc gctggcaaaa gtggcgaata
     4021 accactttgg cattaagtgt tccaactggg acggcccggg ctattaccag gacgacgaca
     4081 agccgaatga atgttttcgg aaatacaact cggtctttga atccttcgac gaccattcga
     4141 atttcttgaa gacccgtccg cgctattcct ttttattcga actcaatcgt accgattaca
     4201 aaggttgggc gcacggtctg aagaaggccg gctacgcaac tgatccgacc tacgcgaacc
     4261 gactcatcaa gatcattgaa gagcacaacc tgcacgtcta cgatcttgca gagaatccgg
     4321 aaaaccggcc gatcgccagc accaacacca tcgttccggt ggaggcacgt cccctcccac
     4381 ccgttgaaaa cacggtacgt gagcaacggg tgtttgtccc cagtgttgag ccggtggaca
     4441 tgttctccaa ccgccgcatc gaacggaaca acggggtgga gttcattcgt gcgcgcaagg
     4501 gcgatacgtt cgagtcactt tcccgtgaat tgagctacgg atattggcaa ttgcctaaat
     4561 acaacgaact gcccgagaac accccgttat tcgaaggaca gatcatttac atcaaaccga
     4621 aaaaacgcga cgcggatact cgtttctacg ttgtaaaacc cggtgatacc gtccattccg
     4681 tttcccagga acttggtatg aagacccgtt ttctttgcaa gtacannnnn nnnnnatcgg
     4741 ccagccttgc accgggcaca cgacttcagg tagcggaata agttccgttc agaacgtgat
     4801 ccttacgaag tacagtttat tctgccggta agcgggcagc agcagcgttt cctcgtcgac
     4861 ctgtttaccg gatcgcggga tgatggcgac gcgctccact tcattgaaaa gcgtttccgt
     4921 ccgttgcgcg ccttttccgt caacgatcga taacagcacg gacgaatcgt cgtcgatgta
     4981 tttattgaag agggaatact gcattccatt ggaaacgacg gaacagtagg acgaatagta
     5041 gccactatcg tcgatcgaat tctggtcctt ttgaaccacg ttgctccagg agatactgcc
     5101 ggcgggatcc atggagatca gcaggatgtt gccgaaatgg taatagtaat gcgtcacact
     5161 ggcctgcatg tagaaatccc aatacgtacg agtggtctgg taaaaggact ccgcgacgat
     5221 caccgcgccc ccgtcgttac gcaggatgat ccggtcgatc gagtagttga ccaactcccc
     5281 actgccccgg tcacggcgat cgccggacgt tttacgcacg aactcctggc tgaagggagc
     5341 cgtcaaaacc cgcgtggtcg cgagcgcatc ctcttccagg ccgtaataga aaacaccgga
     5401 cgtcgtgtag ttggttttat ccgagtaaaa acctgcgacg acaattcggt tattgcggtt
     5461 atcggccgcc agcgaaacat ccgtcagaaa acgatcctgc actttgatct cctgattgac
     5521 caggcgatca ccggaatgct gataacccaa gaggcggtaa tagctctctc ctggcgcttt
     5581 gactttcttg tctgtcagga actcgatgcc aagaatgtag aagttccccc gttcggtgag
     5641 cacataacga agcggtgaaa accggggctg ttcggtatcg accgggatct ctttccggta
     5701 gagcaacgac aatgcagtat cgagcaacac cgcttcgaac gattgtctcg aaccatccga
     5761 tgggactttt cggtaggtga aaacgaactg tgattcgtca cgattcagca gaatacccgg
     5821 cttgttgtct tcatcgagcc tgcttaccgc gacggtatcg atcaggactg gggtgccgat
     5881 gtaggctgca cccgtgccaa gatactgggc atagaagcga aactgtcggg tggacttatc
     5941 ctgcccatag gaaaccacca ggacgcggcc attgaccacc ttgatatctg cgaatcgggt
     6001 atcatccgat tgggcgttga gttcgccttc ccatcgcagc gccatttcca tggaaaagta
     6061 ctgaaggaaa tacttccgac tgcgcagtcc tgcccgatcg cggtcggtat caaggctgat
     6121 gttgctgcgc aatacgaaaa aaccttcgtc atcgcctccg atgatcttga ggtaggggaa
     6181 tttcgggttt tctggaagcg ttggcgacca ctttatctcc ctgccaaagc cgggaaaagc
     6241 accaatcaag gtcaataaaa tcagtgtacg tgaaaaaatt cccagcataa agcactatcc
     6301 atttcggatt ccaaaattac tacttttaag aaggattccg tggactcggc atcaaccctt
     6361 cgatctttat caaatttcaa gaacatgcga caacattcac ttttgacaac aatgtttttc
     6421 ctgttggcgt tctcggcttt tgccactcaa acaactcaag accggaagct tcccttcgaa
     6481 aaagaaagcg aaatcgcagc atcgaatgga ggtttgtcag tccagttcat acttgatgtt
     6541 cccgattgcg attcgctttg tatcggacaa ttctcctgct tcgtgcaagg cggtgtacca
     6601 cccttcactt atagttggtc gaccggctca atagacagtt tcactacggg cgtttgcgca
     6661 caatcccaga tcagtctata cgtcgaggac tccgtaggcg attgggtcaa cacggtatat
     6721 ttcgttccac agcccttacg gttcctggtg acgaccaccc cggccagttg cgcaacatgc
     6781 tgcgacggca gcggacttgg aaccgtcagc acttcgtgct cgctccccgt tacttatctt
     6841 tggaatcctg gcatggttac tcttcccacc ataaataaca tgtgcgggaa tatcacttat
     6901 agcctatgcg ttacggatgt atgtggatgc accttatgcg acacactaat actctgcggg
     6961 tttcctgccg ggacctctaa cctcatcgcc gatcggccag tattccaggc aacctatgca
     7021 tcagccggtc aatgcatcat ggcttcccta ttggatggac catccaccgc gcagttcgac
     7081 ttatatgatc ttcaaggcag aaagatcctg caatcgactc tggagaaaag cgaaaccaga
     7141 agcatcaacg cggccgccct ggcttccgga acttatcttg tcgtggtaag cgatgaaaat
     7201 cgtctgctcg gaaatcaact gctcgtcata ccctgactac ttcctgttcc atgatgcgta
     7261 aatctggtat aagccttctc gtatttcaaa tcttcctgct gatccaagcg gaagcgcaat
     7321 cgcccgacct tacccgtctg accgactgga tggccggatc ttacagcagt gaggcgcagc
     7381 acttacgcga cactgccaac tatttcgaca tccgtttgct gatggctcct atctggaaag
     7441 aacgatcgga tggtcattgg ttttacgtgg agcaggccgt tgccgattac cttgataaac
     7501 cctaccgcca acgggtttac cgcgtccacg aaatcgaacc cggtgttttc gagagcgtca
     7561 tatacaccct gcaggaaccc ttgcgtttca cccaccatcc gga
//
LOCUS       DAOTON010000022         7490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_8663_length_7490_cov_4.505313, whole genome shotgun sequence.
ACCESSION   DAOTON010000022 DAOTON010000000
VERSION     DAOTON010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8663_length_7490_cov_4.505313"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="P5292_00815"
     CDS             <1..488
                     /locus_tag="P5292_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HRS37694.1"
                     /translation="AIMSQQLSTKVADTLHRRFLELYGEAEPTPEQILETPHETLRAI
                     GLSNAKAIYVKEVARHFIEQGLSDALLLKMSDAEVIEELTKIKGVGVWTVQMILMFSL
                     ARPDVFPSGDFGIQQAMIRLYKLRTTDKKKMIARMEKIASAWSPYRTVASMYLWQWKD
                     E"
     gene            complement(592..1578)
                     /locus_tag="P5292_00820"
     CDS             complement(592..1578)
                     /locus_tag="P5292_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018398713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STELLO glycosyltransferase family protein"
                     /protein_id="HRS37695.1"
                     /translation="MTSLIITSISAPNAVLRRFAEACPQHGLDFIVIGDVKSPADFSL
                     PGCRFVSIEEQDTLDFRLARILPKKHYARKNLGYLLAWKSDCIIETDDDNFPYDTFFT
                     PRNRSVEAFTVGGKGWVNAYDYYSDDKLWPRGLPLEAIHNPTPPLSLTRQAVLAPVQQ
                     GLANENPDVDAVFRMTRELPVNFRSAPPVALGEGAWCPFNSQNTTWFPEAFLLLYLPS
                     YCSFRMTDIWRSFVAQRLLWTCGWPMLFHEATVWQERNEHNLLRDFSDEVPGYLHNAA
                     MAHALEELDLKSGQEHLSENLFRCYQTLTERGWVGKEELPLVEAWIQDAEIR"
     gene            complement(1584..2324)
                     /locus_tag="P5292_00825"
     CDS             complement(1584..2324)
                     /locus_tag="P5292_00825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS37696.1"
                     /translation="MSRFPNMNWAVVVPMANEEPDFAPFVEALTAQLDALGSGKVYFV
                     VDNVSKDRTLELCRKLSAQDARFVTIWAPENRCVVDAYLRGYREALRNGHEFILEMDA
                     GLSHDPATLPAFLEKLAGGYECVFGSRFIEGGSMGDSPPKRVLLSRTGTVLSNLLLGT
                     TLHDMTSGYQGFHRRIVERFCEKPLLSRGHFYQTELRYLLRRYKGVEVPIRYKAPSPR
                     VSGGSIRNSLETLLHFWWRRLTFRAQSL"
     gene            complement(2361..4142)
                     /locus_tag="P5292_00830"
     CDS             complement(2361..4142)
                     /locus_tag="P5292_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS37697.1"
                     /translation="MLPLLQVAHLITEFPSDEGTFTAVNDVSFSLQQGETVGIVGESG
                     SGKSVTSLSIMRLLASPGRIRSGSIVYQAPGKEAVDLTRMEEAALCRYRGKEIAMIFQ
                     EPMTSLNPVYSCGFQVAEALILHEKLNKREARERTIELFQEVALPRPEQLYDAYPHQL
                     SGGQKQRVMIAMAMSCRPKVLIADEPTTALDVTVQATILDLMRRLQEEHRMGMLFITH
                     DLGVIAEIAQRVVVMYKGRVVEQGTVQEIFANPKHPYTKSLLACRPPLDRRLRRLPVV
                     NDFLEEQADGSFTDKQGVVENLNAQVISSEERAAQHQGLYKTEPILRVDHLNTWFPIR
                     KGLFGKTNDYVRAVDDVSLVVHPGETLGLVGESGCGKTTLGRSILRLIEPTSGSVHFK
                     GTDFLALQGEELRRMRRHIQIIFQDPYSSLNPRLTIGAAILEPMQVHRLHANDRSRKE
                     KVMDLLRKVGLREEHYDRYPHEFSGGQRQRVCIARALSLEPEFIICDESVSALDVSVQ
                     AQVLNLLNDLKREFGFTYIFISHDLSVVRFMSDRMIVMNKGKIEEQGDPDQIYQSPST
                     EYTKRLIAAIPAADPEAAARKRLLATR"
     gene            4316..4975
                     /locus_tag="P5292_00835"
     CDS             4316..4975
                     /locus_tag="P5292_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37698.1"
                     /translation="MMMSSENQFLRNLYEQLDSAWNQSKISQNPKAARWNYALMGSPI
                     RCGGALMVGFNWGARKGHTYEKQTSIPLKSDDWGSLKRVLPLIEQYFNIGERDLMQTN
                     FCFFRSEKQKDIPEQDIVSCASIFLELIDFIRPKLMICFSTVLRNWLIDHAKVMVGVQ
                     RESVVSKRGNLVVLVGKLRGKSGSYPIVFLPHPNYPLERQSRRLTWEAAVKLLNDFNS
                     R"
     gene            5355..5726
                     /locus_tag="P5292_00840"
     CDS             5355..5726
                     /locus_tag="P5292_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRS37699.1"
                     /translation="MHLGAFSISLNVKDLEASKKFYEALGFSAFAGTKEHRYYIMKNG
                     NALVGLFQGMFDGNMLTFNPGWDESAQTIPGFTDVREIEKKVMAAGFSPVKRVGAGPS
                     GPAHFVISDPDGNVILIDQHV"
     gene            5843..6682
                     /locus_tag="P5292_00845"
     CDS             5843..6682
                     /locus_tag="P5292_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37700.1"
                     /translation="MFSLLLQFTKFIKSKQCILTLLLLLIFSRSDAQIHYIDFNPDIE
                     LSAYPEGNRDTFALDMNEDGVTDFTAAAWSWFQFIHQQCCWNYRAYITGTDTGNAFAD
                     SPSACSPLYLDSGSCFGSTNIYNAIMFGQMGPAGLCEPESINPEYYPFKMLIQNQPHY
                     GWLRVGANVGALTIYDGAINLVSGDSMCAGQVVGLNDAPEVLTLHVFPNPARDIITIE
                     APFPAVGGRLRLLDGFGRILISEQVKSTGPIRLSVDALASGFYFLKLESDRFRAISTV
                     VIR"
     gene            complement(6810..>7490)
                     /locus_tag="P5292_00850"
     CDS             complement(6810..>7490)
                     /locus_tag="P5292_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS37701.1"
                     /translation="EEKTIRNILDKVNEVTLIGGGKKEIVVVNDCSRDKTRETVEAYK
                     SEHPDQHISLFNQPVNMGKGAALRKGIELATGDFIIVQDADLEYDPHEFNLLLRPLIE
                     DKADVVYGSRFMGGRPHRILFFWHSLGNKFLTMLSNAFTNLNLTDMETCYKMFRAPVI
                     KGLQLKENRFGFEPEVTAKMSRVKGVRIYEVGISYYGRTYAEGKKIGWKDGFRAIWCI
                     IKYNLFSR"
BASE COUNT         1826 a         1811 c         2006 g         1847 t
ORIGIN      
        1 ctgccatcat gtcgcaacag ttatccacga aggtggcgga caccttgcac cgaaggtttt
       61 tggaactcta cggcgaagca gagcctacgc ccgaacaaat tctcgaaacc cctcacgaga
      121 ccttgcgcgc aataggcctg tcaaatgcga aggcgatcta tgtaaaagaa gtggcccgac
      181 attttatcga gcagggatta agcgacgcgc tgctcctaaa gatgagcgac gcggaggtga
      241 tcgaagaact gaccaagatc aagggcgttg gcgtatggac cgtgcagatg atcctcatgt
      301 tttcgctcgc ccgcccggat gtgtttccat cgggagattt cggaattcaa caggcaatga
      361 tcaggttgta caaactccgt acgaccgaca agaaaaaaat gatcgctcgt atggagaaga
      421 tcgcttcagc ctggtcgcct tatcggacgg tggcgagcat gtatctgtgg caatggaagg
      481 atgaatgaag tcccaggttc cgggttccgg gtttcgcgtt gaaattggat ttccattatt
      541 gaggactccc catcaaccat cctgcttact tgaaccggaa actcgaaacc atcaacggat
      601 ttccgcgtcc tggatccagg cttctaccag cggtagctct tcttttccga cccagccccg
      661 ttcagtgagt gtctggtagc aacggaataa attttcggaa aggtgttctt gcccggattt
      721 caggtcaagt tcctccagcg catgtgccat ggcggcattg tgcaggtatc ccggcacttc
      781 gtccgagaaa tcgcgcagca ggttgtgttc gttccgttcc tgccaaacgg tcgcttcgtg
      841 aaaaagcatg ggccagccgc aggtccacag cagccgttgt gcgacaaagc tgcgccagat
      901 atcggtcatg cggaaactgc aataggaagg caggtagagc agcaggaaag cttccggaaa
      961 ccaggtagtg ttctggctgt tgaaggggca ccaggcgcct tcgcccagtg ctaccggggg
     1021 cgccgaacga aaattcaccg ggagttcccg tgtcatacga aacaccgcgt caacatcggg
     1081 attttcattg gcaagtccct gctggaccgg agcaaggacg gcttgtcgtg tcagggaaag
     1141 cggaggtgta gggttgtgga tcgcctccag gggcaaccca cgcggccaaa gcttgtcgtc
     1201 ggaataatag tcatacgcat tcacccagcc ttttccgcca acggtgaatg cctcaaccga
     1261 tcggttacgt ggtgtgaaga acgtgtcgta cggaaaatta tcatcatccg tttcgatgat
     1321 gcagtcgctt ttccaggcca gcaggtagcc cagatttttg cgcgcgtagt gtttcttggg
     1381 aagaatccgc gccagccgga agtcgagcgt gtcttgctcc tcgatggata cgaaccggca
     1441 acccggtagc gagaaatcgg caggtgattt tacgtctcca atgacaataa agtcaaggcc
     1501 atgttgagga caggcttccg caaaacgacg cagcacggca ttcggagcgg aaatggaggt
     1561 gatgatcaga ctggtcatac agatcaaagt gattgtgccc gaaaggtaag ccgacgccac
     1621 cagaaatgca gcagggtctc gagtgaattc cggatcgagc cgccggacac ccggggagac
     1681 ggggctttat aacgaatcgg cacttcgact cccttgtatc gacggagcag gtaacgcaat
     1741 tcggtctggt aaaaatgccc gcgtgacaag agtggctttt cacaaaaccg ctcgacgatt
     1801 ctgcggtgaa atccctgata gccggaggtc atatcgtgta gggtcgtacc cagcaacaga
     1861 ttggaaagca ctgtaccggt tcgagaaagc agtacacgtt ttggtggcga gtcccccatc
     1921 gatcccccct caatgaaccg actaccgaat acgcattcgt aacccccggc aagcttttcg
     1981 agaaaggccg gcagtgttgc cggatcgtgt gagaggcctg catccatttc gaggatgaat
     2041 tcgtgaccgt ttcgtaatgc ttccctgtac ccccggaggt aggcatcgac tacgcagcgg
     2101 ttctccggtg cccagatcgt gacaaagcgg gcatcctgtg cactgagttt tctgcaaagt
     2161 tcgagggtcc ggtctttgga aacgttgtca acgacaaagt agaccttgcc cgaaccgagt
     2221 gcatcgagtt gagcggtcaa cgcttcgaca aacggcgcga agtcgggctc ttcattcgcc
     2281 atgggaacca ccacggccca gttcatgttc ggaaacctgg acatacgccg ggcgaaggta
     2341 agaaaaacag cagggtaggg ttaacgtgtg gccaacaagc gcttgcgggc cgccgcttcg
     2401 gggtcggctg ccggtatggc cgcgatcagg cgtttggtgt attcggttga gggcgattga
     2461 taaatctgat cgggatcgcc ttgttcttcg atcttacctt tattcattac gatcattcga
     2521 tcgctcatga agcgtaccac actcagatca tgtgaaatga aaatgtaggt gaatccgaat
     2581 tcccgtttca ggtcattcag caggttaagg acctgggctt gaaccgagac atccagtgca
     2641 gaaacggact cgtcgcagat gatgaattcg ggttccagcg aaagtgcccg cgcgatacag
     2701 acccgctggc gttgcccgcc actgaattca tgcggatagc gatcgtagtg ttcttcgcgc
     2761 aatccgactt tgcgtaacag gtccatcact ttttctttac gggaacgatc gttcgcgtga
     2821 agccggtgga cttgcatcgg ttccagtatc gcggcgccga tggtcaaacg ggggttgagt
     2881 gacgaatacg gatcctgaaa gatgatctgg atgtggcgac gcatcctgcg tagttcctca
     2941 ccctgaagag cgaggaagtc ggtccccttg aaatgtactg aaccggaagt aggttcgata
     3001 agtcggagga tggaacggcc gagggtggtc ttgccgcagc cggattcgcc gaccagtccg
     3061 agtgtttcgc cgggatgaac gaccaggctg acgtcatcga ccgcacgcac atagtcgttg
     3121 gtctttccga ataatccctt tcgaatggga aaccaggtat tcaggtgatc gacccgaagg
     3181 atcggttcgg ttttgtagag cccctggtgc tgggcggcgc gttcttcgga cgatatcacc
     3241 tgcgcattca ggttttcgac cacaccttgc ttgtccgtga atgatccgtc ggcttgttct
     3301 tcgaggaagt cgttcacgac cggaagtcga cgcaaccggc gatcgagcgg cggtcggcag
     3361 gccagcaggc ttttcgtata aggatgtttc gggttggcga agatctcctg tacggtccct
     3421 tgttcgacca cgcgcccttt gtacatgacc acgacgcgct gcgcaatttc cgcaatgaca
     3481 cccaggtcgt gggtgatgaa cagcatgccc atgcgatgct cttcctgcag ccggcgcatg
     3541 aggtcgagga tggtcgcctg taccgtcaca tcgagcgcgg tagtcggttc gtccgcaatg
     3601 agcaccttag gccggcaact catggccatc gcgatcatga cacgttgttt ttgtccgccc
     3661 gacaactggt gcggatacgc gtcataaagt tgttcgggcc ggggtagtgc gacctcttga
     3721 aagagttcga tcgttcgttc gcgtgcctcc cgtttgttga gcttttcgtg caggataagt
     3781 gcctcagcca cctggaagcc gcatgaataa accgggttaa gggaggtcat gggctcctga
     3841 aagatcatgg cgatctcctt gccccgataa cgacacagtg ccgcttcctc catacgcgta
     3901 agatccaccg cttctttacc gggtgcctgg taaacgatgg agcctgagcg gatacgtccc
     3961 ggcgaagcca atagccgcat gatcgagagc gaggttaccg actttcccga accggattca
     4021 cccacgatgc cgacggtctc cccctgttgg agggaaaaac tgacgtcgtt cacagctgtg
     4081 aacgtgcctt catccgaagg gaattcggtg atgagatggg ctacctggag caagggaagc
     4141 atctgtgcta agctagctga tcttcgaata ctccggtaac ttcgcaggat gaattcttca
     4201 cactgagttt gtcgaaaaga tttccatttt taaaaaatga aagagcaatt gaagcttcaa
     4261 tcaatgaagc tggatatttt gtagttttga tcatcgatca aggaatggaa taagaatgat
     4321 gatgagttca gagaatcagt ttctgagaaa tttatacgaa caactggact cggcttggaa
     4381 tcaatcgaaa atttcacaaa atccgaaggc tgcaaggtgg aattacgcct tgatgggaag
     4441 cccaatccgg tgtggcggag cgctgatggt aggctttaat tggggcgcac ggaaagggca
     4501 tacttacgaa aaacaaactt cgattccttt gaaatctgat gattggggtt cattgaaacg
     4561 agttttacct ttaattgaac agtatttcaa tattggagag cgggatttga tgcaaacgaa
     4621 tttttgcttc tttagatcag agaaacagaa ggatattcct gagcaggata ttgtttcctg
     4681 cgcatcaatt tttcttgaac tcattgattt tattagacca aaattaatga tctgtttttc
     4741 aacagttctt agaaattggt taatcgatca tgccaaagta atggtggggg tgcaaaggga
     4801 aagtgtagtt tcaaaaagag gtaatttggt agtattagta ggcaagttaa gaggtaaatc
     4861 tgggagctat ccaattgtgt ttctgcctca cccgaactac ccattagaaa gacaatcaag
     4921 gaggttgacg tgggaagcag cagtaaaatt gttgaatgat ttcaatagca gatgattttt
     4981 atcttaggtt tcatcgcagc tttgttgttc gtttattggg tctattccga gccaagtaac
     5041 cgtgtagata aagttcgaat tggcatgaca caacaagatg ttagaaaact tcttggcccc
     5101 cccagggcat ctgaaggggt gacaataaag caaaattata ggcgagaagc cctattattc
     5161 gggaagcctc agagcatccg cggaagggat gtttttccga tcagaattgt ttttgaaaac
     5221 gacaaggtta ccgagataaa cattaaaagt aaatgagatg ccagcccatt ggcacgattt
     5281 caataggata ttttccaact gctcagttca ttcaggggaa agcgctaact taatgctcct
     5341 aaaactcaaa caccatgcac ctgggagcct tttccatcag cctgaacgta aaagatctcg
     5401 aagccagtaa gaagttctat gaagcgcttg gtttcagcgc gtttgccgga acgaaagagc
     5461 atcggtatta catcatgaaa aacggaaacg cgttggttgg cttgttccag ggtatgtttg
     5521 atggaaacat gctcaccttc aatcccggtt gggatgagag cgcgcagacg attccgggat
     5581 ttaccgatgt tcgcgaaatt gaaaagaagg tgatggccgc gggcttttca ccggtcaagc
     5641 gggtaggggc tggaccgagc gggccggcgc acttcgtgat cagcgatccg gatgggaatg
     5701 tgattctgat cgatcaacat gtgtagaatt atcccttagc ctgagctgta gcttcaacag
     5761 aggatattgc aacatttaga caattatctt tgctaatagt gcgacaatat tacttcagga
     5821 aaaacatctg aaatcttcca tcatgttcag cttgcttctt caatttacca agttcattaa
     5881 gtcgaaacaa tgcatactta cattgttatt gctgctgatc ttttccaggt ccgatgcgca
     5941 gattcactat attgacttca atccggatat cgaattgtcc gcttaccctg aaggaaatcg
     6001 ggacactttt gcattggaca tgaatgagga tggtgtgacg gacttcaccg ctgcggcctg
     6061 gtcgtggttt cagtttattc atcagcagtg ttgttggaat tatcgcgctt acattaccgg
     6121 aaccgacacc ggcaatgcgt ttgcggattc accctccgcc tgtagtccgc tttatcttga
     6181 ttcgggatcc tgttttggtt ccacgaatat ttacaatgcg atcatgttcg gccagatggg
     6241 tcccgccggt ttgtgtgaac cggaatcaat caatcctgaa tattatccat tcaagatgtt
     6301 gattcagaac caaccccatt acggatggct cagagttggt gccaacgttg gggcattgac
     6361 aatctatgac ggtgcgatca acctggtaag tggagattct atgtgtgcgg gtcaggtcgt
     6421 cgggctcaat gatgctccgg aagttctgac cctgcacgtt ttcccgaacc cggccagaga
     6481 catcattact attgaagcac catttccggc tgttggcggc cggttacgcc ttttagacgg
     6541 attcggccgg atacttattt ctgaacaagt gaaatcgaca ggtcctattc gattgtctgt
     6601 cgatgcactg gcttcgggat tttacttttt gaaacttgaa tctgatcggt ttcgagccat
     6661 tagcaccgtg gtgatacgat gatgtgcacc aatcaatttg aaaaactgaa taaccagata
     6721 ctggttaccg ggcaaaaact agaatttccc ggttaggaaa acgcaaagaa ctacccagcg
     6781 gcggttagtg aatagtatcc taccactact caacgcgaaa acaggttgta cttgataata
     6841 caccagatcg cccggaagcc atccttccag ccgatctttt ttccttcggc gtacgtgcgg
     6901 ccataataag agatgcccac ttcatagatc cgcacgcctt ttacccgcga catcttggct
     6961 gtcacctccg gttcaaaacc gaagcggttc tcctttaact gcaatccctt gatcaccggt
     7021 gcacggaaca tcttgtagca ggtttccatg tcggtgaggt tgagattggt gaaggcattc
     7081 gataacatcg tcaggaattt attcccgagc gaatgccaga agaagaggat ccggtgcgga
     7141 cgtccgccca tgaaccggga cccgtagact acgtcggcct tgtcttcgat caaggggcga
     7201 agcaacaaat tgaattcgtg cggatcgtat tccaggtcgg catcttgcac gatgatgaaa
     7261 tcaccggtag ccagctcgat gcccttgcgt agcgcggcgc ctttccccat gttgacgggt
     7321 tggttgaaca ggctgatgtg ctggtccggg tgttcggatt tgtacgcctc gacggtttcc
     7381 cgggtcttgt cgcgcgagca gtcgttgacc accacgatct cttttttccc gccgccaatc
     7441 aacgtcactt cgttaacttt atccaggatg ttgcgaatgg tcttctcctc
//
LOCUS       DAOTON010000023         7490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_8664_length_7490_cov_3.923067, whole genome shotgun sequence.
ACCESSION   DAOTON010000023 DAOTON010000000
VERSION     DAOTON010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8664_length_7490_cov_3.923067"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="P5292_00855"
     CDS             complement(<1..1604)
                     /locus_tag="P5292_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRS37702.1"
                     /translation="MKYNLRRLKTALLVLGTVVVTLVSSAFVDDYFEVSKNLDIFSTL
                     YREVNMYYVDSTDPGKLMRKGIDSMLENLDPYTNYIPESDIEDYRFMTTGQYGGIGAL
                     IRQHGDYIQISDPYEGYPAQLADLRAGDVILEIDGRSAKGKKTDEVSRALKGSPNTDV
                     KLLIRREGEENPILKTITRKEIKVKSVPYASRIGDNIGYIRLSSFTENASGEVGDALK
                     RLKQEGELKGVILDLRGNPGGLLNEAVNISNLFVERGQEIVSTRGKIREMDKSYKAQN
                     NPLDPQLPVAVLVNSGSASASEIVSGSLQDLDRAVVVGQRTFGKGLVQTTRPLSYNAQ
                     LKITTAKYYIPSGRCIQALDYSHRNEDGSVGKIADSLTHEFKTKAGRTVRDGGGILPD
                     LIAESDKLSNIAQSLLSKNLIFDYATRYRRNHPDIPAARDFRLGDADFADFEAFLKDK
                     DYDYSTRSEKTLEELRKIADEEAYLTAVKTEYEALKAKLSHDKQNDLRKNKAEILELL
                     QEEIASRYYYQVGRIEAALQHDPQLN"
     gene            complement(1619..1831)
                     /gene="yidD"
                     /locus_tag="P5292_00860"
     CDS             complement(1619..1831)
                     /gene="yidD"
                     /locus_tag="P5292_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRS37703.1"
                     /translation="MMSRLFIAIIRFYQGALSPLLRPSCRFTPTCSQYGIEAIKKHGA
                     FRGGWLTLKRIARCHPWGGHGHDPVP"
     gene            complement(1821..2225)
                     /gene="rnpA"
                     /locus_tag="P5292_00865"
     CDS             complement(1821..2225)
                     /gene="rnpA"
                     /locus_tag="P5292_00865"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRS37704.1"
                     /translation="MAALRRNTFRKLERLVSRKSFETLVQEGQSVHHPPVRLTWQRQV
                     LSTGFPAATAFSVPKRNFRRAVDRNRIKRLLREAFRKNKAELYTLLEERKQEVALLFV
                     FTGRKLPDYRQVETQVMRCLQQLTGQLRSHDE"
     gene            complement(2238..2987)
                     /locus_tag="P5292_00870"
     CDS             complement(2238..2987)
                     /locus_tag="P5292_00870"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRS37705.1"
                     /translation="MSLKVKTILVTQPKPETDKSPYYDLAKKFSLKIDFRPFIQVEPI
                     PAKDFRKDKVNLAEHTAVILTSRNAADHFFRVCNELRVTVPETLKYFCISESTAYYLQ
                     KFVVYRKRKIFFGKQTISDLMDVIKKHKKEKFLVPCTDIHKQEIFETLDTHHIDYTKA
                     VIYRTVASDLSDLANVNYDVLVFFSPSGITSLLKNFPKFKQNNTRIAAFGPTTAQAVL
                     DAGLRLDIQAPVPAAPSMTMALEQYIKKANK"
     gene            complement(3022..3864)
                     /locus_tag="P5292_00875"
     CDS             complement(3022..3864)
                     /locus_tag="P5292_00875"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HRS37706.1"
                     /translation="MPDTLSLIGLPNDSLAFADSVGVSATDSLVRIVPAPPVKTSPAI
                     INLYGDHALQPTHNGPIDRPYVSPDWLFPILLLIAAVFTWLRVFYNKYFTQLFEAVVN
                     NNLTNQIVRDENILVQRASVYLSIVFYLVGALFLYEFSLHRNWPLAGIGSGFPRFLFF
                     AILVSAVYALKFLILKIAGWLFDVEKETALYIFNVFLLNNLLGILLLPFIALLAFHGA
                     LPGDRLFQAALILIGLVYLYRLFRGFLVGIGTSGFSPLYLFLYLCTLEIAPLLVLLRL
                     ASRS"
     gene            complement(3944..4828)
                     /locus_tag="P5292_00880"
     CDS             complement(3944..4828)
                     /locus_tag="P5292_00880"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRS37707.1"
                     /translation="MVKRLLLLSLLAALCAPAFAQDDLLNQLRLESGSGEHERVMATF
                     KGNHVVNMQSTETVRKHNLDFRVSHLFGTMGTESGGGKHTLYGLDQSNDIRIGLHFGI
                     TDKLMVGVARYKRMENFEGFLKYRILEQTQDDHVPLALTAFSNVALSAVAGEVIERNT
                     DRLTYHSSLIIARKFSPGFSLAAGGGFLHRNITADGDPSDLFSFQAGVRWRFTKSASV
                     LADYSHAFGREELLPDYQDVIGGGLELETGGHVFTIMLTNASGLMENDFLVNTLDSWS
                     KGGMKFSFIISRVFGLKK"
     gene            complement(4828..5394)
                     /locus_tag="P5292_00885"
     CDS             complement(4828..5394)
                     /locus_tag="P5292_00885"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRS37708.1"
                     /translation="MRLSPLAIMLLISFLFVSTCTEGQSVYITDKGFVSFFSEAPIEN
                     IEGRHLGPQSILNLPKRELAFIIPIRGFHFEKSLMEEHFNEKYMESDRFPNATFTGTL
                     SQPIHPGLDTSMAMEAAGKMTIHGVEQSLQIPGKAEVRNGVIRLTASFPIAVKSYGIS
                     IPKLLTENIADTVRVDLDITYKPFRKND"
     gene            complement(5398..5763)
                     /locus_tag="P5292_00890"
     CDS             complement(5398..5763)
                     /locus_tag="P5292_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37709.1"
                     /translation="MRLLFTGLLLLSSLWSCTKDKQTILFVRPAGCDSSLVSYSRQIR
                     PIILNNCAFSGCHVAGGEGAYDYRTYGVLADRIRSGRLLQRLRLDPEDPMKMPEDFEM
                     NPCDRYTLELWIQQGFPDN"
     gene            complement(5806..6597)
                     /locus_tag="P5292_00895"
     CDS             complement(5806..6597)
                     /locus_tag="P5292_00895"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRS37710.1"
                     /translation="MKEYIESGIVELYAMGNLSSTERMEFEQRLLLYPELRAELDRVQ
                     QALEQYAEAHSINPRPSTRRRLMDHVREQQRSESGEAQRDRSFYLTYKYLIAASLAAL
                     FISTFASWFFYSRWNDAEERYTAVLQEKQSLSLNLNTLQASFDKLYADHMIGRDPDVR
                     ILTLAMADSSAKPMARIYWNPRSHETWVEVLEPMPAENGMQYQLWAMEADTPVNAGMF
                     DMPEEDGMFRVKDVAIATAWAVTVEPKGGSERPTMERKVMVTPGS"
     gene            complement(6612..7163)
                     /locus_tag="P5292_00900"
     CDS             complement(6612..7163)
                     /locus_tag="P5292_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRS37711.1"
                     /translation="MVSRKTIPEQELIELLASRDVKGLEYLYDRYSAALYGVIHRIVG
                     VQERSEEILQETFLRIWERVRHYDPAKGRLFTWMLNIARNLAIDAVRSREFREHRQDD
                     PLEKAEHSHTRNGFNPETIGLRNLVDTLDADYREIIDLLYYRGLTQAEAALKLNIPLG
                     TVKTRSRAALLKLRALFETEIEH"
     gene            complement(7222..>7490)
                     /locus_tag="P5292_00905"
     CDS             complement(7222..>7490)
                     /locus_tag="P5292_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HRS37712.1"
                     /translation="AYPIKGTPLYGETEGSFLHAPDWARSTDRDIDFQRTYPRKFYDY
                     AIRRMHNEVAFTRAWVAGGRGIARALVFKMKSLAAKGGMLIHRR"
BASE COUNT         1644 a         1991 c         2095 g         1760 t
ORIGIN      
        1 tcattcagct gcggatcgtg ttgcagggcg gcttcgatac ggccaacctg atagtaataa
       61 cgggaagcga tctcttcctg aagcaattcc aggatctcgg ctttgttctt ccgaagatcg
      121 ttttgcttat cgtgggagag tttggccttc agggcttcgt attccgtctt tacagctgtg
      181 aggtaagcct cttcatcggc gatcttgcgc aattcctcca gcgtcttttc gctgcgggtg
      241 gaataatcgt aatccttgtc tttcaaaaag gcctcgaagt cggcaaagtc agcatcgcct
      301 aatcggaaat ccctggcagc cggtatgtct ggatggttgc gacgatagcg ggtcgcgtaa
      361 tcgaagatca ggtttttgct cagcaggctt tgcgcgatgt tgctgagctt gtccgattcc
      421 gcgatcaggt cgggcagaat accgccaccg tcgcggaccg ttcgccctgc tttggttttg
      481 aattcgtgcg tcagtgaatc cgcgatcttg cctacactgc cgtcctcatt gcggtgggaa
      541 taatccaatg cctgaataca ccggccgctc gggatgtagt atttggcggt cgtgatcttc
      601 aattgcgcat tgtaggagag gggacgcgtc gtttgtacaa ggcctttgcc gaacgtgcgt
      661 tgtccgacca cgacggcacg gtcgaggtcc tgcaatgaac cgctgacaat ctccgaggcg
      721 gacgctgagc cgctgttcac cagtacagcc accggtagct gcgggtccag cggattgttc
      781 tgggccttat aactcttgtc catctcgcgg atcttaccgc gggtgctgac gatctcctgt
      841 ccgcgttcca cgaacagatt ggaaatattg accgcttcgt tcaggagacc gccgggattt
      901 ccgcggagat caagtatgac gccttttagc tcgccttcct gcttcagtcg cttgagcgca
      961 tcgccgacct ccccgcttgc attctccgtg aaactgctga ggcggatgta accgatattg
     1021 tcaccgatcc ggctggcgta gggaacgctt ttcactttga tctctttccg cgtgatcgtt
     1081 ttcaggatcg ggttttcttc gccttcgcgt cggatgagca acttgacgtc cgtattgggt
     1141 gatcctttca gcgcccggct cacttcgtcg gtcttctttc cttttgcact gcggccgtcg
     1201 atttcaagaa tgacgtcgcc cgcccgcaaa tcggccagtt gcgccggata cccttcgtac
     1261 ggatcgctga tctggatgta atctccgtgc tgccggatga gtgcgccgat tccaccgtat
     1321 tgcccggtag tcatgaagcg ataatcttca atgtcggatt ccggaatgta attcgtataa
     1381 ggatccaggt tttccagcat cgagtcgatg cctttccgca tcaacttgcc cggatcggtc
     1441 gaatccacat agtacatgtt cacctcccgg tagagggtgg agaatatgtc caggttcttt
     1501 gacacctcga aatagtcgtc aacaaatgct gaactgacca gcgtgaccac taccgtgccc
     1561 aggaccagca gggcggtttt tagacggcgc agattgtatt tcattccaac gatttttttc
     1621 agggaacagg gtcgtgaccg tgtcctcccc agggatgaca ccgggcgatg cgcttcaagg
     1681 taagccatcc gcctcggaat gcaccgtgct ttttgatcgc ctcaatgccg tattgcgaac
     1741 aggtgggcgt gaaacgacaa ctcggacgga gcaggggcga caaggcaccc tgatagaaac
     1801 ggatgatcgc gatgaatagt ctactcatca tggctccgca attgaccggt cagttgttgc
     1861 agacaacgca tgacctgggt ttccacctgt cggtagtcgg ggagtttccg gccggtaaac
     1921 acgaacagaa gcgcgacctc ttgtttgcgt tcttccagca acgtgtaaag ttccgcttta
     1981 tttttccgga aggcttcgcg caacagccgc ttgatgcgat tccggtccac cgccctgcgg
     2041 aagtttcgtt ttggtactga aaaggccgtg gctgccggaa atcccgtgga aaggacctgt
     2101 cgttgccagg tcaggcgaac agggggatga tggacggatt gaccttcctg aacgagggtt
     2161 tcgaaggact tgcgactgac gagtcgctcc agtttacgga acgtattgcg gcggagcgct
     2221 gccatgccgc ccgggaatta cttgttggct ttcttgatgt actgctccag cgccatcgtc
     2281 atcgaaggcg ctgccggtac gggagcctgg atgtcgagac gaagacccgc atccagcaca
     2341 gcctgcgcgg tggtcggtcc gaaagcggcg atccgggtat tgttctgctt gaacttcggg
     2401 aagttcttca ggagcgaagt gatgccggaa ggggagaaga ataccagcac gtcatagttc
     2461 acattggcca ggtcggatag gtcgctggca acggttcgat agatgaccgc cttggtataa
     2521 tcgatgtgat gagtgtccag ggtctcgaaa atttcctgct tgtggatatc ggtgcaggga
     2581 acgaggaatt tctccttctt gtgcttcttg atgacgtcca tcagatcact gatggtttgc
     2641 ttgccgaaaa agatcttccg cttccggtag acaacgaatt tctgtaggta gtaagcagtc
     2701 gattccgaaa tgcagaagta ctttaacgtt tcgggcacag tgacgcgcaa ttcgttgcaa
     2761 acccggaaaa aatgatcggc ggcgtttcgg ctcgtcagga tgaccgccgt atgctcagcg
     2821 agattgacct tgtcttttcg gaagtctttc gccgggatcg gctcgacctg aatgaacgga
     2881 cggaagtcga tcttgaggct gaacttcttc gccaaatcgt aataaggaga cttatcggtt
     2941 tcaggtttcg gctgagtaac cagaatggtc ttcactttca ggctcatggg ctgcaatttc
     3001 cgtttcggtc gtcagtcgaa atcaactcct tgacgccagg cgcaggagca ccaacaaagg
     3061 ggcgatttca agggtgcaaa gatacaaaaa caaataaagc ggtgagaatc cggaggttcc
     3121 tatcccgacc aggaatccgc gaaacagccg atacagataa accaggccga tcaggatcaa
     3181 ggcggcctgg aacaagcggt ctcccggcag tgctccgtga aaggccaaca atgcgatgaa
     3241 aggcagtaac aggatgccca acaggttgtt caggagaaag acgttgaaga tgtagagggc
     3301 ggtctccttt tctacatcga acagccagcc ggcgattttt aatatcagga atttcaaagc
     3361 atagacggct gacaccagga tcgcgaagaa aagaaagcgg ggaaatccac tgccaatgcc
     3421 ggcaagcggc cagttccgat gcaggctgaa ttcgtacagg aacaaggcgc ctaccaggta
     3481 aaagacgatg ctgagataaa ccgaagcgcg ttgaaccagg atgttctcgt cgcgtacgat
     3541 ctggttcgtc aggttgttgt tgacgaccgc ctcaaagagc tgcgtaaagt acttgttgta
     3601 gaagacgcga agccaggtga acaccgccgc gatcagcagc aggattggaa acaaccaatc
     3661 cggcgaaaca tacggccggt cgatcggtcc gttgtgtgtc ggctgcagcg cgtggtcacc
     3721 gtacagattg atgatcgccg ggctggtctt gaccggcggg gccggcacga tccgaaccag
     3781 actgtcggta gcgctcaccc cgacagagtc ggcaaaggcc aggctgtcgt tcggcaaacc
     3841 tatcaggctc aaggtgtccg gcatgccgcg aaggtaagca gtttacgcgc tgtcaggaat
     3901 accgtctgga agagtttccc gttccggaat gtgctgtccc ggattatttc ttcaacccga
     3961 aaacgcgcga gatgatgaaa ctgaatttca ttcccccctt gctccaggaa tcaagcgtgt
     4021 tcacgagaaa atcgttttcc atcaatccac ttgcattggt cagcatgatc gtgaagacat
     4081 gcccgcctgt ttccagttcc agcccgccac cgatcacatc ctgataatcc ggcagaagtt
     4141 cttcgcggcc gaaggcgtgc gagtaatcgg cgagaacgga tgcgcttttg gtgaatcgcc
     4201 agcgaacacc ggcctgaaaa ctgaacagat ccgaaggatc gccatccgca gtaatgttgc
     4261 gatgaaggaa acctccaccg gcagcaagcg agaagccggg agagaatttt cgggcgatga
     4321 tcagcgaact gtgataggtc aaccggtcgg tgtttcgctc aatcacttcc ccggcgacag
     4381 ccgaaagcgc tacgttggag aatgcggtca gcgcgagggg aacgtgatca tcctgcgttt
     4441 gctccaggat gcggtacttc aggaatcctt caaagttctc catgcgtttg tagcgggcca
     4501 caccgaccat caacttgtcg gtgatgccaa aatgcagccc gatccggatg tcattcgact
     4561 ggtccagccc atacaaggtg tgctttcctc cgccgctttc cgttcccatg gtgccgaaca
     4621 aatgactgac acggaaatcc aggttgtgct ttcggacggt ttcggtgctc tgcatgttca
     4681 ccacatggtt gcccttgaag gtggccatga cgcgctcgtg ctcacccgac ccgctctcaa
     4741 gtcgcaactg attcaacagg tcgtcttgcg cgaacgccgg tgcgcacaaa gcggccagca
     4801 acgatagcag aaggagtcgt ttgaccatca gtcgttttta cgaaatggct tgtaggtaat
     4861 gtccagatcc acgcggaccg tatccgcaat gttctccgta agaagttttg ggatggagat
     4921 cccgtaagac ttgaccgcga ttggaaagct ggcagtcaga cggatcacgc cattccgaac
     4981 ttcggcttta ccgggaatct gcaggctttg ctcgacgccg tggatggtca tctttcccgc
     5041 ggcttccatg gccatcgacg tgtcaaggcc cggatggatc ggttggctga gggttcccgt
     5101 gaaggtggcg ttcggaaacc ggtcgctctc catgtacttc tcgttgaaat gctcctccat
     5161 cagcgacttt tcaaaatgaa atccccggat cggaatgatg aatgctagct cacgtttggg
     5221 aaggttcagg atcgattgcg gtccgaggtg gcggccttcg atattctcaa tcggcgcttc
     5281 ggaaaagaat gaaacgaatc ctttatcggt aatgtagacg ctttgccctt ctgtgcatgt
     5341 tgaaacgaac aggaaggaaa tcagcagcat gatggcgaga ggggagagcc gcatggttca
     5401 gttgtctgga aatccctgtt gtatccataa ttcaagcgtg taccggtcgc agggattcat
     5461 ctcgaaatct tccggcatct tcatcgggtc ttccggatcc aaccgaagtc gctgcaacaa
     5521 tcgaccgctc cggatgcggt cggccagtac cccgtaggtc cggtagtcgt acgcgccctc
     5581 gcctccggcc acatgacaac cgctgaaagc gcagttgttc aggatgatcg gacggatctg
     5641 acgactgtaa ctgaccaggc tgctgtcgca tccggcaggc cggacgaaca agatcgtttg
     5701 cttatccttg gtacacgacc aaagcgagct aagcaggagc aggccggtga acaggaggcg
     5761 catgactcct tcaaagatag aaaaaggtaa acgcgtcggt aggctttaac tacccggtgt
     5821 gaccatgacc tttcgttcca tggtgggtcg ttcgcttccg cctttcggct ccacggtgac
     5881 ggcccaggcg gtcgcgatgg cgacgtcctt tacccggaac ataccgtcct cctccggcat
     5941 atcgaacatg ccggcattga caggcgtgtc ggcctccatg gcccacaact ggtattgcat
     6001 gccgttttcg gccggcatgg gttccaatac ttcgacccag gtctcgtggg agcgcgggtt
     6061 ccagtagatg cgtgccatcg gtttcgcgct ggagtcggcc atggcaagtg tcaggatcct
     6121 gacatcggga tcacgtccga tcatgtggtc cgcatacagt ttatcgaagg aagcctggag
     6181 cgtattcaga ttcagcgaaa gtgattgttt ttcctgcagc accgccgtat agcgctcttc
     6241 ggcatcgttc cagcgggaat aaaagaacca ggatgcgaag gtgctgatga acaaggccgc
     6301 gagactcgcc gcgatcaggt atttataggt gaggtagaag gagcggtcac gctgtgcttc
     6361 cccggattcg gaacgttgtt gttcacgcac gtgatccatc agccgtcgac gcgtcgaggg
     6421 tcgtggattg atggaatgcg cctcggcata ctgttcaagt gcctgctgga cccgatccaa
     6481 ttcagctcgc aattccggat acaggagcag gcgctgctcg aactccatgc gctcggtcga
     6541 cgagaggttg cccatcgcgt acagctccac aattccggat tctatgtact ccttcacgtc
     6601 catgcttccg ttcaatgctc tatttctgtt tcaaacaagg cccgcaactt cagcagcgcc
     6661 gcccgcgatc gggtcttgac ggttcccagc ggaatgttca gtttcagcgc cgcctcggcc
     6721 tgggtcaaac cgcggtaata cagcagatcg atgatctccc ggtaatccgc gtcaagggta
     6781 tctacgaggt ttcgtagccc gatggtttcc gggttaaagc cgtttcgggt gtgggaatgt
     6841 tccgcttttt ccagcggatc gtcttgccgg tgttccctga attcgcgtga gcgaacggca
     6901 tcgatcgcca ggttgcgcgc aatgttcaac atccaggtga acaaacggcc cttggcagga
     6961 tcataatgac ggacccgctc ccaaatgcgc aaaaaagttt cctgcaggat ctcctctgaa
     7021 cgttcctgca cgcccacaat gcgatgtatc acaccgtata aggcagccga atagcgatcg
     7081 tacaggtatt ccagtccctt gacatcgcgg gatgcaagca gttcgatcag ttcctgttcg
     7141 ggaatcgtct ttctggaaac cactggtaag gggtgaatgt ggaggttaaa gaaacgaaaa
     7201 agccccggct ttattccggt ttcaacggcg gtgtatcaac ataccgcctt tggctgccaa
     7261 tgatttcatc ttgaatacca aagcacgggc aattccacgt cccccggcga cccatgcgcg
     7321 tgtgaaggca acttcattgt gcatcctcct gatcgcgtag tcatagaatt ttctcggata
     7381 ggttctttga aaatcaatat cccggtcggt tgatcgggcc caatccggag catgaaggaa
     7441 ggatccctcc gtttcgccgt acaacggagt gcctttgatc gggtaggcaa
//
LOCUS       DAOTON010000024         7481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_8684_length_7481_cov_3.487207, whole genome shotgun sequence.
ACCESSION   DAOTON010000024 DAOTON010000000
VERSION     DAOTON010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8684_length_7481_cov_3.487207"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..252)
                     /locus_tag="P5292_00910"
     CDS             complement(67..252)
                     /locus_tag="P5292_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37713.1"
                     /translation="MFEYTKQILTKVSFDRNLFRKELVKALQLLKKEERRMLKIWCVA
                     SFAAYSDIILEVYRKVY"
     gene            complement(427..996)
                     /locus_tag="P5292_00915"
     CDS             complement(427..996)
                     /locus_tag="P5292_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRS37714.1"
                     /translation="MTSARKIVVAVTGASGAIYAKVLFDKLTRLHDQIGEVGVVFSDN
                     AQDVWKFELGNESYRELPFKTYGKSDFMAPFASGSARFDTMIICPCSMGTLARIAAGT
                     SNDLITRAADVILKERRRLILVPRDTPYSLIHIRNMQTVTEAGAIVCPASPSFYSRPA
                     DFEALAATVVDRVIDLAGLQQSSYRWNES"
     gene            complement(1077..1478)
                     /locus_tag="P5292_00920"
     CDS             complement(1077..1478)
                     /locus_tag="P5292_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRS37715.1"
                     /translation="MKLKALAPALVWAFIVLVLCGIPGSRLPELSFWQWLRPDKIVHL
                     VLFGTQSYLLLVGFTGIPVTSRWHQQAGWRAVTISILYGALLEWLQAHVFIQRSGDVR
                     DAIANALGAILGWWLFSRFHQRLLRRLRREA"
     gene            complement(1485..1865)
                     /gene="gcvH"
                     /locus_tag="P5292_00925"
     CDS             complement(1485..1865)
                     /gene="gcvH"
                     /locus_tag="P5292_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRS37716.1"
                     /translation="MNFPDNLKYTKDHEWVRVDGNIATIGITDFAQSELGDIVYVDIN
                     TVGDTLDKEAVFGTVEAVKTVSDLFMPVSGKVLEMNPGLDGSPEKVNKDPYGDGWMIR
                     VEMTNASEAAGLMSAADYKSSIGA"
     gene            complement(1921..7449)
                     /gene="sprA"
                     /locus_tag="P5292_00930"
     CDS             complement(1921..7449)
                     /gene="sprA"
                     /locus_tag="P5292_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRS37717.1"
                     /translation="MRVLNLDDLNPNGDPQPDGVFDFVDGLTINRQTGRIIFPVLEPF
                     GSSLRAKFCGDSLLADYYCYDPLYDSTKVAAQQIPEKNKFSLRGTYQSSSGSDIPLNA
                     INIPQGSVKVTAGGVPLRENVDYTVDYTLGRVKIINDGLLKSNTPIRVSLESQSLFNL
                     QQKTYIGNRFDYTFNKDFQVGATVLHLNERPLTQKVNIYDEPISNTVVGVDGTYRTDS
                     RFLTRLLDKLPFYNTKETSTLTFSGEVAKLFPGHNKAIGQNGVSYLDDFEGAITPLDL
                     RVPGNWYLASAPQGQVQPGMFPEAQYNDSLVYGYNRAKVAWYYVDPLFQRDQPGITPA
                     DIDDDDQSNNFVREIPQREIFPNVSQPTGPQVITCMNLAYYPTERGPYNYDVDSVPGL
                     SAGIDTDGRLRDPASRWGGIMRRLETTDFEASNIEFIQFWMMDPFATGTENDGSGGEL
                     YFNLGNLSEDILRDGHKSFENGLPAPTNNYTTENSAWGRYPVVQTVVNAFDNDPASRA
                     SQDIGLDGLTTTDERNFFQTSYIDRVAARYGTGSVAYQLAVQDPSSDDYAYFLNPDYS
                     TTNTAPLDRYKRFNGHEGNSPANGTIDGVQSTATTLPDMEDVNRDNNMEIGENYFQYK
                     VLLKPANMVVGQNYITDMIESPVRYANNTTGTVKWYQFKVPVRSPDQVIGTVDVQNIR
                     FIRMFMKGFQTPVVARFARLELLRGEWRPYIYSLLNTGEYAPTPEVPGDTKFDVGVVS
                     IEENGSRIPVPYVLPPGIDRERDVSATQLATINEASLSMRVCELSDGDGRAAYRNTQF
                     DLRSYKKLRMFVHAESRGASDGLQNGDLHMFVRLGTDLSDNYYEYDIPLVVTPWGATA
                     RDQVWPEANNMEIELNKLVQAKLARNNAMQVNGTVTLRTPFYVQDGDRTITIKGSPNL
                     SNVRSLMIGVRNPKDPSGTGPKLCAEVWVNELRLSDFDENGGWAATARINAKLADLGN
                     MTIVGNHSAAGWGSIEKKVSERDRQDKTAYDFSTNIELGKFLPEKSGVKVPMYFGYSE
                     QFIKPQFNPLDPDVPLNQAIDAIPNAEGRDSLRKQTIDYTQRKSLNFTNVRKTKTGAA
                     PKTRIYDVENLNFTYAYTEIYQRNYNIAYSLYKTHSGLIGYNYNKTGKPIQPFEKVPG
                     KLINSKWMKPVKEFNFNPLPSSLTFSTALDRTYSETQLRNNSGLLFSIDPLFIKTFIM
                     QRRYGLNWDLTRSLKLDFNADANAIIDEPPGKLDTREERDSVRTNLMRLGRLQRYNHT
                     SNLTYNLPFNKIPATDWVSGNVRYGATYNWQTSPLYRDSVTNEIVPNPFANTIQNSQT
                     IAYNANLTFTTLYNKVPFLKKINQGAQRPAADRQPKPKIKSPTDSLNKAPKDSLREKK
                     SPLEPVFRGLANLLMSVKSGNITYTETNGTLLPGFRGKPDYLGMDFNYAGNSNAPGWG
                     FLFGSQRDIRPDGIRNNWFTFDTTLSSFFTRTQLQNLTARLTVEPVKSFRIELTGNRN
                     YTLNRTENFRYGSDGQFRSYSPVENGNFSISYLTWNTHFIKDANDYSNKNFDDFRAYR
                     SQMSGLLAAENSNYVSGSDTISGFKNGYGPAQQEVVAYAFLAAYTGRKPSDRFIDRFP
                     KIPRPNWRITYDGLSKLKFFQKFLQSFSLSHGYRSIYSINSFNQNLLYSEAGGDPFKR
                     DTVGNFIPRYDMQQITIAEQLAPLIGIDMTWKNSLQTRFEIKRDRTLTLAFSNIQVTE
                     VRGTEYTLGLGYKFKRVTLPFRTGNGRQKLSNDLNVRADFNWRENTTILRKVIENTNQ
                     PSAGSTVLSMKFNVDYPVNDRFNVRAFYEYSSNNPFVSSTFPTSTTFAGFAIRFTLAQ
                     "
BASE COUNT         1510 a         1921 c         2279 g         1771 t
ORIGIN      
        1 gggtctttag aaattcagca gtaaagcgta cgggctaccg tggactgatg cggaacctgg
       61 tgttcctcag taaaccttcc ggtaaacttc cagaatgata tcggaatacg cggcgaaaga
      121 ggccacgcac catatcttca acattcttcg ttcttccttc ttcagcaatt gaagagcttt
      181 caccaactcc ttccggaaga ggttccggtc gaaactaacc ttggttagaa tctgcttcgt
      241 gtattcgaac atgttgatcc gttaaggtga cgtgaattta gccgtaagcc gaattagtgt
      301 caagcgattt aacgttaaac cttacccttg aaggaaacgt gtgaaagcca attttattct
      361 aattaccgga tttcaacagg atcggttgaa aaacaggctg ttgcacggaa cttgttggag
      421 gcccgctcag gactcgttcc agcggtagga agattgttgc agaccggcca ggtcgatgac
      481 gcggtccact accgtagccg caagcgcttc gaaatcagca ggtcgggagt agaagctggg
      541 acttgcggga caaacgatcg cacctgcttc cgttacggtt tgcatattcc ggatgtggat
      601 caggctgtag ggggtatccc tgggaacgag gatcagtctc cggcgttcct tcaggataac
      661 atcggctgcc cgcgtgatga ggtcgttgct ggtacccgca gcaatccggg ccagtgtccc
      721 catcgaacat gggcaaatga tcatggtatc gaagcgggcg gagccggaag caaagggggc
      781 catgaagtcg ctcttgccat atgttttgaa tggcaattca cggtaggatt cattccccaa
      841 ttcgaatttc cagacatcct gtgcattgtc ggaaaagact accccgactt ccccgatttg
      901 atcatgcaga cgtgtcagtt tgtcgaagag taccttggcg tagatcgcgc cactggcacc
      961 ggtgaccgca acaacgattt ttctggcgga ggtcatggga gttagaacga ttggagtccc
     1021 gcattgttca tgcgtaagcg taccaggtta aagataagca atcggatggc gcggcatcag
     1081 gcctcccgcc gcaatcgccg aagcaggcgc tggtggaacc tggaaaagag ccaccagccc
     1141 aggatggcgc cgagagcatt ggcgatcgca tcccggacat ctccggagcg ctgaatgaaa
     1201 acgtgggcct ggagccattc taggagggct ccataaagga tgctgatcgt gaccgcgcgc
     1261 cagccggcct gttgatgcca gcggctggtg acagggatac ctgtaaaacc aaccagcaac
     1321 aggtacgact gcgtaccgaa aagtacgagg tgtacgatct tatcgggccg aagccattgc
     1381 caaaaactca gttcagggag tcggctgccc gggatcccgc aaaggaccag tacgatgaaa
     1441 gcccacacca gcgcgggcgc aagggctttc agcttcatgc cggatcaggc gccgatgctg
     1501 gatttgtagt cggctgcgga catcagaccg gcagcttcgg atgcgttggt catttcgacg
     1561 cggatcatcc aaccatcgcc gtaaggatcc ttgttcactt tttcgggact gccatccagg
     1621 ccgggattca tttcgagcac cttgccgctg accggcatga aaagatccga tacggtcttc
     1681 acggcttcaa cggtaccgaa gaccgcttcc ttgtccagtg tgtcgccgac cgtattgatg
     1741 tctacataca cgatatcgcc cagttcactt tgagcgaaat ccgtaatgcc gatggtggcg
     1801 atgtttccat ctacgcggac ccattcgtgg tccttggtgt acttcagatt gtccgggaaa
     1861 ttcatggccc caaacgtacg tactttttcg gaatccggga tggcccggga ccggccgtcc
     1921 ttactgggcg agggtgaacc ggatcgcgaa tccggcgaag gtggtcgagg tcgggaaggt
     1981 ggaggagacg aacggattgt tcgaactgta ctcgtaaaag gcccgaacgt taaaccggtc
     2041 gttgacggga taatccacgt tgaacttcat gctcagcacg gtgctgcccg cgctcggctg
     2101 gttcgtgttc tcgatcacct tgcgcaggat ggtggtattc tcccgccagt tgaaatccgc
     2161 acgcacattc aggtcgttgc tgagtttttg ccggccgttg ccggtccgga acggaagcgt
     2221 cacccgcttg aatttatagc ccagtccgag ggtgtactcc gtaccgcgaa cttccgtgac
     2281 ctggatgttg gagaaggcaa gagtcagggt gcggtcgcgc ttgatctcaa agcgagtctg
     2341 caggctgttc ttccaggtca tgtcgatgcc gatcagcggc gccaattgtt ccgcgatcgt
     2401 gatctgttgc atgtcgtagc gcgggatgaa gttgccaacc gtatcacgtt tgaaaggatc
     2461 tccgccggcc tcgctgtata gcagattctg gttaaaggag ttgatgctgt agatcgagcg
     2521 gtagccgtga gaaagcgaga aggactgcag gaatttctgg aagaacttca gcttcgacaa
     2581 gccgtcgtag gtgatacgcc agttcgggcg cgggatcttc gggaagcgat caatgaaccg
     2641 atccgatggc ttccgaccgg tataagctgc caggaaagcg taggcgacca cctcctgttg
     2701 cgcgggacca taaccgttct tgaaaccgga aatggtatcg ctgccggata cgtagttgct
     2761 gttttcagca gcgagtagcc ccgacatctg ggaacgatac gcgcggaagt catcgaagtt
     2821 cttattcgaa tagtcgttgg cgtccttgat aaaatgggta ttccacgtca ggtacgagat
     2881 cgagaagttg ccgttctcca ccggactgta actgcggaat tggccgtccg atccgtaacg
     2941 gaagttttcg gttcggttaa gtgtgtagtt gcggttgccg gtcagttcga tccggaagct
     3001 cttcacgggt tcgacagtca gtcgggcggt caggttctgc agttgcgttc gggtaaagaa
     3061 cgaactgagt gtcgtatcga aggtgaacca gttgttccgt atcccgtcgg gtcggatgtc
     3121 gcgctggctg ccgaagagga aaccccatcc gggagcgttc gagttcccgg cataattgaa
     3181 gtccatgccc aggtaatcag gcttgccgcg gaaacccggc agcagcgtac cgttggtctc
     3241 ggtataggtg atgttacccg acttgacgct catgagcagg ttggccaatc cgcggaacac
     3301 cggttcaagc ggtgactttt tctcccgcag actgtccttc ggagccttgt tcaggctgtc
     3361 ggtcgggctc ttgattttcg gtttcggctg tcggtcggcc gctggtcgct gagcaccctg
     3421 gttgatcttc ttcaggaagg gcactttgtt gtacagcgtg gtaaaggtca ggttggcgtt
     3481 ataagcgatc gtctgcgagt tctggatcgt gttggcgaac gggttgggaa cgatctcgtt
     3541 ggtcacgctg tcgcgataca gtggcgaggt ctgccaattg taagttgcgc cgtagcgcac
     3601 attacccgaa acccaatcgg tcgccgggat cttgttgaac ggaaggttgt aggtcaggtt
     3661 cgaggtgtgg ttgtaccgtt gcagacgacc caggcgcatc aggttggtcc gaaccgaatc
     3721 ccgttcctcg cgggtatcga gttttcccgg aggctcatcg atgatcgcgt ttgcatcggc
     3781 gttgaaatcg agtttcagcg aacgggtgag gtcccagttc aggccatagc ggcgctgcat
     3841 gatgaaggtc ttgatgaaca acggatcgat gctgaagagc aatccgctgt tgttccggag
     3901 ttgggtttcc gaatacgtac ggtccagggc ggtgctgaag gtcaggctgg acggaagcgg
     3961 gttgaagttg aactccttca ccggcttcat ccacttcgag ttgatcagct tgccagggac
     4021 tttctcgaac ggctggatcg gtttaccggt cttgttgtag ttgtacccga tcaggccgga
     4081 atgggtcttg tacagactat acgcgatgtt gtaattacgc tggtagattt cggtgtaggc
     4141 gtaggtgaag ttcaggttct ctacatcgta gatcctggtc ttgggagcgg ctcccgtctt
     4201 ggtcttgcgg acgttcgtga agttgaggct cttccgttgc gtataatcga tcgtctgctt
     4261 ccgcaacgaa tcgcgtcctt cggcgttcgg gatcgcgtcg attgcctggt tgagcggaac
     4321 gtcggggtcc agcggattga attggggctt gatgaattgc tccgagtaac cgaagtacat
     4381 cggaaccttg acacccgatt tttccggcag gaattttccg agctcgatgt tggtcgagaa
     4441 gtcgtaagcg gttttatcct gacggtcgcg ttcactcact ttcttttcaa tgcttcccca
     4501 gcccgccgca ctgtggtttc cgacgatggt catgtttccg agatcggcca gcttcgcatt
     4561 gatgcgtgcg gtggctgccc agccgccgtt ctcgtcgaag tcggacaagc gcaattcgtt
     4621 cacccagact tccgcgcaca atttcggccc ggtccccgag gggtctttcg ggttgcggac
     4681 accgatcatc agtgaacgca cgttgctgag attcggagat cccttgatgg tgatcgtacg
     4741 gtcaccatcc tgaacgtaga agggggtacg aagagttacc gtcccgttca cctgcatcgc
     4801 gttgttgcgg gcgagttttg cctgcacgag tttgttcaac tcgatctcca tgttgttcgc
     4861 ttccggccaa acctgatcac gcgcggtagc tccccagggc gttacgacga gcggaatatc
     4921 gtattcgtag tagttgtcgc tcaggtcggt tccgagtcgg acgaacatgt gcaggtcgcc
     4981 gttttgcaac ccatcggatg cgccgcggga ttccgcgtga acgaacatcc ggagcttttt
     5041 gtaagagcgg agatcgaatt gggtgttgcg ataagcggca cgaccgtcgc cgtcggaaag
     5101 ttcgcaaacg cgcatggaga gcgaggcttc gttgatcgta gccaactgcg tggccgatac
     5161 atcccgctcc cgatcgatac cgggaggcag cacgtacggg accggaatgc ggctgccgtt
     5221 ttcttcgatg ctgacgacgc cgacatcaaa cttggtgtct ccgggaactt ccggtgtggg
     5281 cgcgtattcg ccggtgttga gcagcgaata aatgtagggg cgccattcgc cgcggaggag
     5341 ttccagacgg gcgaagcggg cgacaaccgg tgtctggaag cctttcatga acatccggat
     5401 gaagcggatg ttctgtacgt cgaccgttcc gatgacctga tcggggctgc gtactggaac
     5461 cttgaactgg taccacttca cggtgccggt ggtgttgttc gcgtagcgca ccggcgattc
     5521 gatcatgtcg gtgatgtagt tctgacctac caccatgttc gccggcttga gcaggacttt
     5581 gtactggaag tagttctcgc caatctccat gttgttatcg cggttcacgt cttccatgtc
     5641 aggaagcgtg gtcgcggtgg attggacacc gtcaatggtt ccattggccg gcgagttacc
     5701 ctcgtgtccg ttgaatcgtt tgtagcggtc gagcggcgct gtgttcgtgg tactgtaatc
     5761 cgggttgagg aagtaggcgt agtcgtcgct ggacgggtcc tgcaccgcga gctggtaggc
     5821 gaccgatccg gttccgtaac gcgctgcaac acggtcaatg tagctcgtct ggaagaaatt
     5881 ccgttcgtcc gtcgtggtaa gaccgtcgag gccgatgtcc tgggacgcgc gactggccgg
     5941 atcgttgtcg aacgcgttga cgaccgtttg aacgacagga taacggcccc aggctgaatt
     6001 ctccgtcgtg tagttgttcg tcggagcggg cagaccgttt tcgaaggatt tatgtccgtc
     6061 gcgcaggatg tcttcggaca ggttgccgag gttgaaatag agttcaccgc ccgaaccgtc
     6121 gttctctgta ccggttgcga agggatccat catccagaac tggatgaact cgatgttgct
     6181 cgcttcgaag tcggtggttt ccagacgacg catgattcca ccccaacgtg aagcagggtc
     6241 gcgcagtcgg ccgtcggtat cgataccagc ggaaagcccc ggaacactgt cgacgtcgta
     6301 attgtagggg ccgcgttcgg tggggtaata ggctaagttc atgcaggtga tgacctgcgg
     6361 accggtaggc tgcgaaacgt tcgggaagat ctcgcgctgc ggaatctccc gtacaaagtt
     6421 gttgctctgg tcgtcgtcgt cgatgtcggc gggcgtaata ccgggctggt cgcgttggaa
     6481 cagcgggtcg acatagtacc aggcgacctt cgcgcggttg taaccgtaaa cgaggctgtc
     6541 gttgtattgc gcttccggga acatgcccgg ttgcacttgc ccctgcggag cgctggcgag
     6601 gtaccagttg ccgggaaccc gcaggtcaag cggagtgatg gcgccttcga agtcgtcgag
     6661 ataggagaca ccgttctggc cgattgcctt gttgtgaccc gggaacagct tcgctacttc
     6721 gccgctgaaa gtcagcgtcg aggtttcttt cgtgttgtag aagggcagct tatccagcag
     6781 gcgagtgagg aagcgcgaat cggtccgata ggttccgtcg acaccaacga cggtgttcga
     6841 aatcggttcg tcgtagatgt tcaccttctg ggtgagcgga cgctcgttca ggtggaggac
     6901 ggttgcgcct acctggaagt ccttgttgaa ggtgtagtcg aatcggttac cgatgtaggt
     6961 cttttgctgc agattgaata gcgactggct ttcgagcgac acccggatcg gcgtattcga
     7021 tttgagcagt ccgtcgttga tgatcttcac ccgaccgagc gtgtagtcga ccgtataatc
     7081 cacgttttcc cgcagcggca caccgccggc ggtaaccttc accgatccct gcggaatgtt
     7141 gatggcgttc aacggaatgt ccgagcccga agaggattgg tacgtaccac gcagactgaa
     7201 cttgttcttt tccggaatct gctgggcggc gactttcgtc gaatcgtaga gcggatcgta
     7261 gcaatagtaa tccgccaaca gggaatcgcc gcagaacttg gctcgcaaag aactgccgaa
     7321 cggttcgagg acggggaaga tgatgcgacc cgtttgccgg ttgatggtca atccgtcgac
     7381 gaagtcgaat acgccatcgg gttgcgggtc gccgttcggg ttaaggtcgt ccaggttcag
     7441 gacgcgcacg ccgccggtcg ccatcgcccc catcgccaac t
//
LOCUS       DAOTON010000025         7472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_8693_length_7472_cov_4.325469, whole genome shotgun sequence.
ACCESSION   DAOTON010000025 DAOTON010000000
VERSION     DAOTON010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8693_length_7472_cov_4.325469"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="P5292_00935"
     CDS             complement(<1..538)
                     /locus_tag="P5292_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRS37718.1"
                     /translation="MNISELSLRRPVLATVMNLVIILFGIIGMTFLGVREYPAIDPPT
                     ITVSTNYAGASADIVESQITEPLEKAINGIPGIRTISSTSSTGNSNISVEFNLDAELE
                     AAANDVRDKVSQAVRNLPQDIDSPPVVTKADANSDFIILLALQSQTKGLLELSDYAEN
                     VLQNKFQTIPDVSGVNIFG"
     gene            complement(557..1597)
                     /locus_tag="P5292_00940"
     CDS             complement(557..1597)
                     /locus_tag="P5292_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRS37719.1"
                     /translation="MRYRFLFLPLSILIFSSCKQSGPPAGAGGPAPAAVDVMIAGMDT
                     VPQDIEVNGTILAQESVELRPETSGRIIALEMEDGAVVSKGALLARVNDAELRAQLRQ
                     QEVQLELAQKNLARLNQLLSINGVNQADIDAATSQVASLDAAVKVTQAQLDKTEIRAP
                     FAGRLGLRQVSPGAYVTPATLLTTLQATDRIKVDFAVPETYGRNLKKGMSVFLEDMNG
                     NSSIASVSAIEPLVNANTRNIKVRAVLQGNDLVPGSFVKVRLRQSADGILVPTASIIP
                     DALSSKVVLVREGKGKFVNVKTGVRTADFVEITEGLTPGDSVVVTGVLFVRPNAPVKV
                     RGVRTRSSFTAS"
     gene            1799..2905
                     /locus_tag="P5292_00945"
     CDS             1799..2905
                     /locus_tag="P5292_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRS37720.1"
                     /translation="MPKIRSITLYAARIPLVDPFIISLGMLDHAENVVVRIEADALTG
                     FGECSPFRTIHGETQAGCMSVGKEIAKVLLGADASNPVDCLSRMDKLIFGNACIKSAF
                     DMAIHDLAAQAAGLPLWQYLGGKAGEELFTDYTVSIGPVDRMVKDAVSIRERGFPVVK
                     VKLGEHAAADIQRMRAIRAAIGNDLPIRIDANQGWTKSDAIEALQQMESLNIQHCEEP
                     VSRRAFFELPEIRKNAPIPIMADESCCDEYDAQNLLALQAVDSFNIKLSKSSGLIRAR
                     RIAVLAEQAGMRLQLGGFLESRLGFTAAAHFAWTLQETPYVDFDTPLMLSADPVDGGM
                     LYGPGGRIRLPEGPGLGAAVDAAFLQSCPREEVR"
     gene            2951..3334
                     /locus_tag="P5292_00950"
     CDS             2951..3334
                     /locus_tag="P5292_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS37721.1"
                     /translation="MKQSVYIVDDDPLFAQMLHDHITADGMRKVRVFHSGEECLQHLF
                     EKPNTIILDYHLDSVQKSAANGLAILEKIRKHDRNVHIIVLSSQTRYAVAAETIAKGA
                     EQYVIKDDEAFSKIDAMLQEFARED"
     gene            3410..4885
                     /locus_tag="P5292_00955"
     CDS             3410..4885
                     /locus_tag="P5292_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRS37722.1"
                     /translation="MKHGFSTLVLLLSLLFNATAGEPVLVGDTAVRIDQWLQQLEKEK
                     HFSGGVLVMSKGATILRKGYGWANQAQRIPFDERTLACMGSITKTFTAMAVLKLEEQG
                     KLKVTDTLGKFFSKVPSDKSGITLHQLMTHSGGFAEIFENDGGDYAKLTKEEFLQRAF
                     REPLMYQPGSRSLYSNVGMSVAAAVIEEVSHQSFESFVRNELLQPVGIGDIAYDYPDS
                     ETRRIAHGYDQGTDWGTLMDRYREFGGPYWNLYGNGGFYASISAMETFLRAILDEKIL
                     TPAAIAKQQTKHIEEWDTEGRSFFGYGCALGQSRRGTPVIQNGGSNGIYFADMLIYPK
                     DDVALYVVTNDNRMIANRVMAGITQLVFLGKIEQDPLVVQARFESPLAEAMYDAIGKR
                     GAAEFRKNGNGLIDSLGVGDNDMILLEVGQQLSRENRREETIALYELYTQRYPSIVVA
                     WNDLGDAYRAVGRKTDARRCYEQALKLRPGNPRATEALKLL"
     gene            5131..6132
                     /locus_tag="P5292_00960"
     CDS             5131..6132
                     /locus_tag="P5292_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37723.1"
                     /translation="MARKWNVQGLYLAPIFLLASLTLFLTSCQKEDLLTESNTAARMA
                     STSSPTQVQVLPELFGFYTHPNNLTIGNAASEDNVLNYLRNKGGNMLNCYARNSMATA
                     TERAKFAAFCRKAVSTYGIRLITCDIRETTELPTWDALYTAYPDLRTVVAPLTEKEPW
                     VTNDYAGCFSLLRLTKAMCNRYGAKFYFYEGWMGKNYSNPQAAVDSMVYYCDGIFLSN
                     YVSTADYLSTAPGYGKWDNRIKYRLDQGSSTYGGITKAAKKFNKLDYPIIELQSLQQS
                     FLFSIYATTGSYRSFYGGLYNDAKSLYNASNSDVLKYTELRGKSLFYHPYVLQAQPN"
     gene            6351..6563
                     /locus_tag="P5292_00965"
     CDS             6351..6563
                     /locus_tag="P5292_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37724.1"
                     /translation="MKSRSDLVKAITRTRDLIKITTQEVGELYLEYLETRTEPLLETA
                     RQKAVFIGQLKTRVTELENAFLTADH"
     gene            6721..7398
                     /locus_tag="P5292_00970"
     CDS             6721..7398
                     /locus_tag="P5292_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRS37725.1"
                     /translation="MKAPHPALSRLATIYRSSGDAVVAEGMSAYMKGLFPFVGIKKPQ
                     RAELSKPFFTELKNLDADTMRASVRWLWKQPEREFQYFAMELLWKNKKAWEDTYLELF
                     EELVESKSWWDTVDFIAATLVGHFLTKHPKRIATTTKAWCASGNLWLNRTAILFQLKY
                     KDKTDFALLQRLIEPHRGSKEFFHQKAIGWSLRQYAYTDPKAVVAYVKRTELKPLSRR
                     EAMKHLG"
BASE COUNT         1770 a         2030 c         2020 g         1652 t
ORIGIN      
        1 gtccgaagat gttcacgccg ctgacatccg ggatcgtctg gaacttgttc tgcaggacgt
       61 tctcggcata gtcgctgagt tccagcaagc ctttcgtctg gctctgcaag gcgaggagaa
      121 tgatgaaatc cgaattcgcg tcggctttgg tgacgaccgg aggcgagtcg atgtcttgcg
      181 gaagatttcg caccgcttgg ctcaccttgt cgcgtacatc gttcgcggcg gcttcgagct
      241 cggcatcgag gttgaactcg accgagatgt tcgagttgcc ggttgaactg gtcgacgaga
      301 tggtccgaat acccggaata ccgttgatcg ctttctccag cggttcggtg atctgactct
      361 cgacaatgtc ggcactcgcg ccggcataat tggtcgatac ggtgatcgtc ggcggatcga
      421 tggcgggata ctcacgcacc ccgaggaagg tcattccgat gatgccgaac aggatgatga
      481 ccaggttcat caccgtggcc agtaccggtc gtcgcaggga aagctcggaa atattcatag
      541 gcttcgcggc gaaagatcag gatgcggtga aagaagaacg ggtacgaacg ccgcggactt
      601 tgaccggcgc attgggtcgc acgaacaata caccggtaac gaccacggaa tcacccggcg
      661 tcagtccttc ggtgatctcc acgaaatcag cggtacgcac gccggtttta acattcacga
      721 atttgccttt cccttcccgt accaacacca ccttactgga cagagcgtcg ggaatgatgg
      781 aagccgtggg aaccaggatc ccgtcggccg actggcgcag ccggaccttt acaaaggagc
      841 cgggaacgag gtcattccct tgcagcaccg cgcgcacttt gatgttacgc gtattggcat
      901 tcaccagcgg ttcgatggcc gacacactgg cgatgctgga gttaccgttc atgtcttcga
      961 gaaataccga catgcccttc ttcaggttgc ggccgtatgt ttccggaaca gcgaaatcga
     1021 ctttgatacg atcggtcgcc tggagggtag tgagcagggt tgccggcgtg acgtaggcac
     1081 cggggctgac ttgtcgcaag cccaggcgac cggcgaacgg agcgcggatc tctgttttgt
     1141 ccaattgtgc ctgtgtgacc tttaccgcag cgtccagtga tgcaacctgg ctggttgccg
     1201 cgtcaatgtc ggcctggttg acgccattga tggacagcaa ctgattcaac cgcgccaggt
     1261 tcttttgcgc gagttcgagt tgaacctcct gttgccgcaa ctgggcgcgc aattcggcgt
     1321 cgttgacacg tgccagcaat gcgcctttgc tcaccactgc accgtcctcc atctccaggg
     1381 cgatgatgcg gccactggtt tccggacgca actcgacgga ctcttgcgcc aatatggttc
     1441 cgttgacctc gatatcctgc ggaacggtat ccatcccggc gatcatcaca tcgacagccg
     1501 ccggcgcagg accacctgct cctgccggag ggccggattg tttacacgaa ctgaagatca
     1561 ggatggaaag cgggaggaat aaaaaccggt aacgcatgcg aggaacggat gaggctcaaa
     1621 ggtaacaaaa acagaggcgc atggtttgac tatacgggtt ctgagtttac acgcttcatc
     1681 agaaatttag gaatacgaac atcatcggaa gataatccat tctttcctcc ctgacttgtg
     1741 gataattgca aagccgacac gcaagcgggt cttgaagcga ccggtaattt gcacacgcat
     1801 gccgaagatc cgttccatca cgctttatgc ggcccgtatc ccgcttgtcg accccttcat
     1861 catctccctc ggtatgctcg atcatgcgga aaacgtcgtg gtcaggatcg aagcggacgc
     1921 gttgaccgga ttcggcgaat gcagtccttt ccgcaccatt cacggcgaga cgcaggccgg
     1981 ctgcatgagt gtcgggaaag aaatcgcgaa agtattgctt ggcgccgatg caagtaatcc
     2041 ggtcgactgt ctttcgcgca tggacaaact catcttcggc aatgcctgca tcaagagcgc
     2101 gttcgatatg gccatccacg atctggcggc gcaagctgcc ggattaccgt tatggcaata
     2161 cctcggtgga aaagccggcg aggagctctt tacggattac accgtcagca tcggtccggt
     2221 ggaccggatg gtgaaggatg ccgtatcgat ccgggaacgc ggatttccgg tggtgaaagt
     2281 gaaacttggt gagcacgcag cggcagacat ccaacggatg cgcgcgatcc gagcagcaat
     2341 cggcaacgat cttcccattc gcatcgacgc gaatcagggg tggacaaaaa gcgatgccat
     2401 cgaagcgttg caacaaatgg agtcgctgaa catccagcat tgcgaagagc ccgtctcacg
     2461 tcgtgccttc ttcgagctgc ccgagatccg gaaaaacgct ccgatcccga tcatggccga
     2521 tgagtcctgt tgcgacgagt atgacgcgca aaatctgtta gccctgcaag cggtcgatag
     2581 ttttaacatc aagctgagta aatcctcggg gcttatccgg gcgcgacgca tcgcggtact
     2641 ggcggagcag gccgggatgc gcctgcaact cggtgggttt ctggagtcgc gcctgggctt
     2701 caccgccgca gcacacttcg cctggacgct gcaagagacg ccgtatgtcg acttcgacac
     2761 cccgctgatg ttgtcggccg atccggtgga tggcgggatg ctgtatggcc cgggcggccg
     2821 tatccggctt ccggaaggtc ccgggctggg ggccgccgtg gacgcggctt tcctgcagtc
     2881 ctgcccccgg gaagaggtcc gctgagccgc atttcgggcc tgtcccggca tttcgtacct
     2941 ttcgctcagg atgaaacaat cggtctatat cgtcgacgac gatcccctct tcgcgcagat
     3001 gttacacgat catatcactg ccgatggcat gcgaaaagtg cgcgtattcc attccggtga
     3061 agagtgcctg caacatcttt tcgagaagcc gaacacgatc atcctcgatt accacctgga
     3121 ctcggtacaa aaatccgccg ccaacggact tgccatcctt gaaaagatcc gcaagcacga
     3181 ccggaatgtc cacatcatcg tcctcagtag ccagacccgc tacgcggtcg ccgcggaaac
     3241 catcgcgaaa ggcgccgaac agtatgtcat caaagacgac gaggcctttt cgaagatcga
     3301 tgccatgctg caggaattcg cgcgggaaga ctgataaccg cttcccgact tcaccaactt
     3361 taggattccc ttaacgcgga aggcctttca ttcgccctat actagcgaaa tgaaacacgg
     3421 cttctccaca ctcgtcctgc tgctttccct tctcttcaac gcaaccgccg gcgaaccggt
     3481 attggttggc gacacagccg tacgcatcga ccagtggctg cagcaactgg aaaaagaaaa
     3541 gcacttctcc ggcggagtgc tcgtgatgag caaaggggcg accatcctcc gaaaaggcta
     3601 cggatgggcg aaccaggcgc agcgcattcc attcgacgag cggacactgg cctgtatggg
     3661 ctccatcact aaaactttca ccgccatggc ggtcctgaaa ctggaggagc agggaaaact
     3721 gaaggtgacg gacacgctgg gaaaattctt ttcaaaggtt ccttccgata aatccggcat
     3781 tactctgcac cagttgatga cgcattccgg aggttttgcg gagatctttg aaaacgatgg
     3841 aggagattac gcaaagctga ccaaggaaga atttctacag cgcgcctttc gcgaaccctt
     3901 gatgtaccaa ccgggtagtc gttccctgta ctccaatgta ggcatgagcg tggcagccgc
     3961 ggtgatcgag gaagtcagtc accagtcgtt tgaatcgttt gtcagaaatg aactgctcca
     4021 accggtcggc atcggggaca tcgcgtacga ttatcccgac agtgaaacgc gtcgcatcgc
     4081 tcacggctac gaccaaggca cggactgggg aacgctgatg gaccgttacc gggaattcgg
     4141 aggaccttac tggaatcttt acggcaacgg cgggttctat gcatccatca gcgcgatgga
     4201 aaccttcctg cgcgccatcc tggatgaaaa gatcctaacg cctgccgcga ttgccaaaca
     4261 acagacgaag catatcgaag aatgggacac tgaaggccga tcgttcttcg gctatggttg
     4321 cgccctggga caatcgcgcc ggggaacgcc ggtgatccag aatggcggca gcaacggcat
     4381 ctactttgcc gacatgctga tctatccgaa agacgatgtt gccttgtatg tcgtaaccaa
     4441 tgacaaccgg atgatcgcaa accgcgtaat ggcaggcatt acccaactgg tgttcctggg
     4501 taagatcgag caggacccgc tcgtggtgca agcgcgcttt gagagcccgc tcgcggaagc
     4561 gatgtacgat gcgattggga agcgcggagc agcagaattc cgcaagaacg gaaacggact
     4621 gatcgactca ctgggcgtgg gtgacaacga catgattctc ctggaagtcg ggcagcagct
     4681 atcgcgggaa aatcgccggg aagaaacgat cgcgttgtat gagctctata cgcaacgcta
     4741 cccgtccatc gtcgttgcct ggaatgatct gggagacgct taccgggccg tcggtcgaaa
     4801 aacggacgcc cgccgatgct atgaacaagc cttgaaactg cgtccgggaa acccccgtgc
     4861 tacggaagcg ctgaaattgc tctaaaaaac ggagtgtttc ccggtaaaaa acacattcga
     4921 aatctcattt cgaattgccc ttcctgttga cccgattcat atagaagcca aactctgtat
     4981 ctaacatcgc atcctcggaa acacgagtac tagcaagggt ttcacggaca tcaacccgat
     5041 gttctcccgg atggatgagt gaatatccgt agtacgcgac aaatccgaga tcgctggctt
     5101 aattaaccga ctaaacagga aacaaagaaa atggcaagga agtggaacgt tcagggactg
     5161 tatctcgctc cgatcttttt actggcaagt cttacacttt tccttacatc gtgtcaaaag
     5221 gaagacctgc tgaccgaatc caatactgcg gcaagaatgg cttcaacgtc atccccgacg
     5281 caagttcaag tcctgcctga attgttcgga ttttatactc atccgaacaa tctgaccatc
     5341 ggaaatgccg ccagtgaaga caatgtcctg aattacctgc ggaataaagg tggcaacatg
     5401 ctgaattgtt acgcgcgcaa ttccatggcc actgctacgg aaagagccaa gttcgccgcc
     5461 ttttgccgca aggctgtcag cacgtacggc attcgattga tcacctgcga catccgggag
     5521 acgaccgagc ttcctacctg ggatgctttg tacaccgctt atcctgatct gcgaaccgtg
     5581 gttgctccgc ttaccgaaaa agagccgtgg gttacgaatg attatgccgg ctgtttctcg
     5641 ctgttacgat tgaccaaggc gatgtgcaat cgctatggcg ccaaattcta tttctacgag
     5701 ggttggatgg ggaagaatta ttccaatccc caggcggcag tagattccat ggtgtactat
     5761 tgtgacggta tcttcctgtc gaactacgtc agcacggcag attatctctc aaccgcgccg
     5821 ggttatggca agtgggacaa caggatcaag taccgactcg atcagggatc ttccacttac
     5881 ggcggaatta ccaaggcagc caagaaattc aacaagctcg attatccgat catcgaactg
     5941 caatcactcc aacagtcgtt ccttttctcg atctatgcta ccacaggatc ttatcggtcc
     6001 ttttatggag gcttgtataa cgacgcgaaa tcgctttaca atgcatcaaa ttcagacgtc
     6061 ttaaagtaca ccgagctcag aggtaagagt ttgttttatc acccgtacgt attgcaagca
     6121 cagccgaatt gattcggagt gtacccggac ggatccgaag attcatcttc atcgtcagta
     6181 gttttaagac cgggtggatg tttccaatag ctggaaacat ccacccggtt cttttctaca
     6241 tgcaagcagc cggtttttaa cggggattca tgcgctaccg ttaccataga tgacttttag
     6301 catatccggc ccttgccggc tgccgtacat ttgcattagt aaaatacgct atgaaatccc
     6361 gttccgacct cgtcaaagcc ataacacgta cgcgcgatct gatcaagatc acgacacagg
     6421 aagtaggcga gctatatctt gagtacctgg aaacccgtac cgagcccttg ctcgaaaccg
     6481 cacgacagaa agccgttttc atcggacaac tcaaaacccg ggttaccgaa ttggagaacg
     6541 cgttcctgac ggccgatcat tgattcaggg ttttaattgg ttaaaggttt ttcgtgataa
     6601 cacattttcg ttatcgtttt tcctctgtgc gcactttgtg tcctgcgtgg ttaagtccct
     6661 gatgaatctt tgcttcgatc agctaacgat ttactcggtt ccttttagga aattcgtggc
     6721 gtgaaagcac cacaccccgc cctttcccga ctggccacca tctatcgatc ttccggcgat
     6781 gccgtcgtag ccgaaggcat gtcggcttac atgaaaggac tcttcccctt tgtcggcatc
     6841 aagaagccgc aacgggcgga attgtcgaag ccgtttttta ccgagctgaa gaacctggat
     6901 gccgacacga tgcgggcttc ggtgcgctgg ttgtggaaac aacccgaacg cgagttccag
     6961 tacttcgcga tggaactctt gtggaagaac aagaaagcct gggaggatac ctacctcgaa
     7021 cttttcgaag aactggtcga gtcgaaatcc tggtgggaca cggtggattt catcgcggca
     7081 acactggtcg gacattttct cacgaaacat cctaaacgca ttgccacgac gacaaaagcc
     7141 tggtgcgctt ccggcaatct gtggctgaac cggacggcga tcctcttcca gctcaagtac
     7201 aaggacaaaa ccgatttcgc gctgttgcag cgactgatcg aaccgcatcg cggatcgaag
     7261 gagttctttc accagaaggc gatcggctgg agtttgcggc agtatgctta caccgacccg
     7321 aaggcggtcg ttgcttatgt gaaacgtacc gagctgaagc ctttgagccg gcgggaggcg
     7381 atgaagcacc ttggttgatc cagatattgt tcgatcactt catcttagaa cacggaatac
     7441 acggatatca cggatttaca cggatcttaa ta
//
LOCUS       DAOTON010000026         7270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_8973_length_7270_cov_3.761192, whole genome shotgun sequence.
ACCESSION   DAOTON010000026 DAOTON010000000
VERSION     DAOTON010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8973_length_7270_cov_3.761192"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="P5292_00975"
     CDS             complement(<1..371)
                     /locus_tag="P5292_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HRS37726.1"
                     /translation="MSSLKQGLHQKLLQKLSPQQIQLMKLLQIPVANLEQRIKEELQE
                     NPALEEGQEHDEDEYRADTEGEAEDDNNDDEELSEREEDISLDEYLVDDEIPDYKTSA
                     NNTSPDEERREVPIGMQQNFQ"
     gene            complement(449..1492)
                     /locus_tag="P5292_00980"
     CDS             complement(449..1492)
                     /locus_tag="P5292_00980"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRS37727.1"
                     /translation="MRSLPDHIAGLLADHDCVIVPALGAFLASREPAKVDVSGRRLLP
                     PRRRIAFNVFLRQNDGLLAQRLVETEHFTYPEAVRHVEHFASGVIRKLENGEEFNIAR
                     VGILSMGSDAAIRFAPESESSLDPDCFGLTPVAIPHRDLTASRKSTEANPEPLSVPKN
                     RKTQQRSLAKYQLQQLLSAAAVAGALLWFSFNVYLVTRDGGQQAAMPSLDSVRPDVII
                     NDAAQQPKVLEKSEAKPETIYVASVAPVKSPEPAPEVRSPDVKPQEPAPQPVSSNNQH
                     YFVIAGAFRQPGNAETLVRELQGRGFTNARVIDTVGALLYVCYDGFADANAAEAMLTD
                     LRSRQEKGWIYRN"
     gene            complement(1579..2028)
                     /locus_tag="P5292_00985"
     CDS             complement(1579..2028)
                     /locus_tag="P5292_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRS37728.1"
                     /translation="MRLEDEIAQKQFRNQAHKLVVNIIYTYNWLMNYQQTMLKPHGIT
                     GQQFNVLRILRGQHPNPASIKLVRERMLDKMSDASRIVEKLRMKGLVERHTCPTDRRV
                     CDVTITAKGLALLQILDKENEKVNEMTERLTEDERQTVNDLLDKMRG"
     gene            complement(2428..3138)
                     /locus_tag="P5292_00990"
     CDS             complement(2428..3138)
                     /locus_tag="P5292_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRS37729.1"
                     /translation="MKTVFHPTEQRGHADHGWLNAHHSFSFAGWYDPSRVHFGMLRVL
                     NDDIVAGGQGFGLHPHNDMEIITIILDGALQHKDNMGNGSVIRPGDVQVMSAGTGVMH
                     SEFNPLPDQATNLFQLWIFPKEKGIAPRYDQKSFDAAARNGQFQLVASGFRENGELYI
                     HQDAGISLGDVKGGDTITYRMRKPGNGAYVMLIDGEVNIGDQAMRRRDALGVWDTDTI
                     RINATQNSSLMVVEVPMR"
     gene            complement(3152..3742)
                     /locus_tag="P5292_00995"
     CDS             complement(3152..3742)
                     /locus_tag="P5292_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016862989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRS37730.1"
                     /translation="MKNVPVHELISKRRSTRAFSDRTISDETLLQLFEAARWAPSSFN
                     EQPWRFILARRSDTRAFEAMLDCLNESNRSWAQHGNVFILPVAKLFTTHNHYHNRHAF
                     HDVGLAVANLSLEATAHDIYLHQLGGFHIDKAREHFAIPDEYEPVSIIVLGYAGDISG
                     LPDNLRQREEKPRQRLPLEQLVYSEKFGQSNDLPQQ"
     gene            complement(3848..4288)
                     /locus_tag="P5292_01000"
     CDS             complement(3848..4288)
                     /locus_tag="P5292_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HRS37731.1"
                     /translation="MSNLRPMTKCYSNGEITIVWKHHLCIHSGNCARGLASVFKPKES
                     PWIDPAGADSQSIIDQVAKCPSGALSIELKQSENNSVMENNSLTRVTVTDKGPYLVKG
                     TFVFVDKDGKEETKEGNIALCRCGLSKNKPFCDGTHRSSDVLSK"
     gene            complement(4295..4579)
                     /locus_tag="P5292_01005"
     CDS             complement(4295..4579)
                     /locus_tag="P5292_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37732.1"
                     /translation="MIRLRFPAFPTQEFLQLIPAQRAIVDRLLRQGVIRTYSLTGDRR
                     ELWIVLRAENESAMQTVLNKLPLSEFAQITAQEAMFHLDSVELFPEPSIN"
     gene            complement(4645..5223)
                     /locus_tag="P5292_01010"
     CDS             complement(4645..5223)
                     /locus_tag="P5292_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRS37733.1"
                     /translation="MKKVLTLIAAVALSATAIAQNWKLDGSHSKIRFTTKYLVISDVD
                     GEFKKFEGTFTSSKADWSDLQATMTVQVPSITTDNEMRDKHLLSDDFFNAEKFPTMTF
                     TSTGIKSLGNNKYVLSGNLTVRDVTKKVEVPLVYGGQVKDPWGNLKAGFKATGKINRK
                     DFGLKYANAAATGEAVVGDEVEFTIDAVLIKQ"
     gene            complement(5356..6354)
                     /gene="trpS"
                     /locus_tag="P5292_01015"
     CDS             complement(5356..6354)
                     /gene="trpS"
                     /locus_tag="P5292_01015"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRS37734.1"
                     /translation="MKETVVSGIRSTGNLHLGNYFGAISNFLKMQHEYNCYFFIADYH
                     SLTTHPTPSDLHANVRQVVAEYLACGLDPEVSTLYVQSDLPEIAELYLLLGMNAYVGE
                     LERCTSFKEKIRKQPDNINAGLLTYPVLMAADILIHRAVKVPVGKDQEQHLEMARTFG
                     NRFNRLYHCELFPEPYAFNYGTQLVKIPGLDGSGKMGKSEGEGNAIFLYDEPEVIRKK
                     VMRAVTDSGPTQPNQEKPEAIRNIFTLLEVVSSPDTVAHFNDAYNNMAIRYGDLKKQL
                     AEDMIAFTAPIREKIREMLSDEARLSKVIRHGAEKARESAGKTVREARHAIGIRHF"
     gene            complement(6407..7108)
                     /locus_tag="P5292_01020"
     CDS             complement(6407..7108)
                     /locus_tag="P5292_01020"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRS37735.1"
                     /translation="MIQLRFIAFLLLSLYLTAATPVDFYQDQLRAPRVANAIKVHRST
                     LTDDLNKFGIGPNDVQLLIVAYKAEQELEVYVRRTSEKRYRRFATFPICASSGMLGPK
                     KRKGDGQVPEGVYHLDRFNPYSNFHLSLGLNYPNAEDRQRAGRLDPGGDIFIHGSCVT
                     IGCMPMTDPVIERIYLLAIWSRRNGQAKIPVYSFPFRMNEANLARYSRLFPEHAVFWR
                     SLAEKSRDLEQNLLL"
BASE COUNT         1490 a         1798 c         2141 g         1841 t
ORIGIN      
        1 tcctggaagt tctgctgcat cccgatcggc acctcgcggc gctcttcgtc gggtgaggta
       61 ttgttagccg aagtcttgta atccgggatc tcgtcgtcga ccaggtattc gtcgaggctg
      121 atatcttctt cgcgttcgct cagttcctcg tcgtcgttgt tgtcgtcttc ggcttcgcct
      181 tcggtatccg cccggtattc gtcttcgtcg tgctcctgac cttcttccag ggcggggttc
      241 tcctgcaact cttctttgat gcgctgctcc aggttcgcga ccggaatttg cagcagcttc
      301 atgagttgga tctgctgcgg agagagtttt tgcagcagtt tctggtgaag accttgtttg
      361 agcgaggaca taaagaaagc agggggctct tggcccgtgt gaaattacgg aattggtatc
      421 gttgtaagtt tacggaaaac cgaaggggtc agttgcggta aatccaaccc ttttcctgcc
      481 tggaacgcag gtcggtaagc atggcctcgg cagcgttcgc gtccgcgaaa ccgtcatagc
      541 agacatacaa cagcgccccg accgtatcga tgacgcgggc gttggtgaat ccgcggccct
      601 ggagttcgcg gaccagcgtt tcggcgttac ccggttgccg gaaagcgccg gcaatgacaa
      661 aatagtgttg attgttgctg ctgacgggct ggggtgcggg ctcctgcggt ttaacatccg
      721 gtgatcggac ttccggcgca ggttcgggcg atttcacggg tgcgacactt gcaacgtaga
      781 tcgtctccgg cttcgcttcg cttttttcga ggactttcgg ctgttgggct gcgtcgttga
      841 tgatcacgtc gggtcgcacc gaatccagcg acggcatggc agcctgttga ccaccgtcac
      901 gggtaacgag gtagacgtta aaggagaacc acaggagcgc gccggcgacg gctgccgcgc
      961 tcaacaactg ttggagctga tacttagcga gtgaacgttg ctgagtctta cggtttttcg
     1021 ggacggatag cggttccggg ttagcttcgg ttgatttacg ggaagcagtc aggtcgcgat
     1081 gcgggatggc tacaggagta agtccgaagc agtcggggtc gagtgaggat tcagattcag
     1141 gtgcaaaacg aatcgcagca tcgctgccca tcgacaggat tccgacacgg gcgatattga
     1201 actcctctcc gttctcgagt ttgcgaataa ctccggaagc aaaatgctcg acgtggcgga
     1261 cggcttcagg gtaggtgaaa tgttccgtct ccaccagacg ttgagccagg aggccgtcgt
     1321 tctgtcgcag aaatacgttg aaagcgatgc ggcgacgggg aggaagcaac cggcgaccgg
     1381 atacatccac tttggccggt tcgcggctgg cgagaaatgc tcccaaagcg ggaacgatca
     1441 cgcagtcgtg atcggccaat aagccggcga tatggtcagg aagggaacgc atcgaagttg
     1501 ctgggacgaa attaagataa aaaaaagcgc gtgtccaacg cgcttgtcaa atttgtgata
     1561 aagaagctgt tggggggctc atccccgcat cttgtccagc aggtcgttga cggtctgtcg
     1621 ctcgtcctcg gtgagccgtt cggtcatttc gttcaccttt tcgttctctt tgtcgagtat
     1681 ctgaagcaac gcaagaccct ttgccgtaat ggtcacatcg cacacccggc gatcggtcgg
     1741 acaagtgtgg cgttccacca ggcctttcat ccgaagtttt tccacgatgc gcgaagcgtc
     1801 actcatcttg tccagcatcc gttcgcgtac cagtttgatg ctggcaggat tcgggtgctg
     1861 accgcgcagg atgcgcagga cgttgaactg ttgcccggtg atgccgtgtg gtttcagcat
     1921 cgtttgctgg tagttcatca accagttata ggtgtaaatg atattaacca cgagcttatg
     1981 tgcttgattc cggaattgct tctgggctat ttcgtcttcg aggcgcatgg acgggggggg
     2041 agtgaacagt gaacagtgaa cagtaaacag taaatagtaa atagtaaata gtgggtggtg
     2101 tctggtttac ggtttctggt tttcgggtaa ttgcaatatc gtcttgccgt taaggtttag
     2161 ccgccactca gctactgtga aatggagtat atattgcctt cgttccactg taatcgtcaa
     2221 gtttctttac caacgcatcc gccgtgtcga atactgcagg ttgtggagcg agccaaacac
     2281 gatagcgaag ttagtaactg gaaactggta attagaaatt agtaactagt aattagtaat
     2341 taaactgttt gcttcggcct acgccaaagc ttcaacaggc aaaattcgct attcactgtt
     2401 tactgtttac tgttcactat tcactcctca ccgcataggg acctccacta ccatcaagct
     2461 gctgttttgt gtcgcattga tacggatcgt atcggtatcc cagacgccca gcgcgtcgcg
     2521 tcgacgcatg gcttgatcgc cgatgttgac ttctccgtcg atgagcatga cgtaggcgcc
     2581 gttccctggc ttgcgcatac gataggtgat ggtgtcgcct ccttttacat cacccaggga
     2641 aataccggca tcctggtgga tgtacaattc cccgttttcc cggaagccgg aagcgacgag
     2701 ctggaactgt ccgttacgag cggcggcgtc gaatgatttc tgatcgtaac gtggagcaat
     2761 gcctttctcc ttcggaaaga tccaaagctg gaagaggttg gtggcctggt cgggcagcgg
     2821 gttgaactcg gaatgcatca cgccggtgcc ggcgctcatt acctgtacat cgcccggacg
     2881 aatgaccgaa ccgttgccca tgttgtcctt atgctgaagc gcaccatcga ggatgatggt
     2941 gatgatctcc atgtcgttgt gcgggtgcag gccaaagcct tgtccgcctg caacgatatc
     3001 gtcgttcagc acgcgcagca tgccgaaatg aacgcgtgac ggatcgtacc agccggcgaa
     3061 gctgaaacta tgatgggcat tcagccagcc gtgatcggca tggccgcgct gttcggtggg
     3121 atggaatacg gttttcatag gcttgttcct ttcattgttg aggcagatca tttgactgcc
     3181 cgaatttttc tgaatagacc agttgttcga gcggcaggcg ttgtcgcggc ttctcttcac
     3241 gctgtcgcag gttgtccgga agtccggaga tatcgccggc ataaccgagg acgatgatgc
     3301 tgaccggttc gtattcgtcc ggaatggcga agtgttcccg tgctttatcg atatggaagc
     3361 cgcccaactg gtggaggtag atgtcgtgcg cggtggcttc cagactgagg ttggcgaccg
     3421 ccaggcctac atcgtggaag gcgtggcgat tgtggtagtg gttgtgcgtg gtgaagagct
     3481 ttgccaccgg aaggatgaag acgttgccgt gttgcgccca ggagcggttc gattcgttca
     3541 ggcaatcgag catcgcttcg aacgcccggg tgtcggaacg acgggccagg atgaagcgcc
     3601 agggttgctc gttgaaactc gaaggagccc aacgggccgc ttcgaacaac tgcagcaggg
     3661 tttcatcgga gatggtccgg tccgaaaacg cgcgcgtgct gcgacgtttg ctgatgagtt
     3721 cgtgtacggg aacgtttttc atggtttcta tttgttcatc ggtatcagcg gccggaggcg
     3781 gatcccggca agggtcctca ccacacacca caggtgcccc cggtcgcgct gatacggatg
     3841 ctgcgcatca cttcgagagc acatcgctgc tgcggtgcgt accgtcgcag aatggtttgt
     3901 tcttggacag tccgcagcga cagagggcga tgtttccttc cttcgtttct tcttttccgt
     3961 ctttgtcgac gaatacaaag gtgcccttga cgaggtacgg tcctttgtcg gtaacagtga
     4021 cgcgggtaag tgaattgttt tccataacgc tattgttttc tgattgtttg agttcaatgg
     4081 agagtgcacc gctcggacac ttcgccacct gatcgatgat cgactggctg tcggcccctg
     4141 ccggatcaat ccagggcgac tccttcggct tgaacacgga cgcgagtccg cgtgcgcagt
     4201 tgccggaatg gatacacagg tggtgtttcc agacgatggt gatctcaccg ttggagtagc
     4261 acttggtcat cgggcgaagg ttcgacatga cggatcagtt gatggaaggt tcggggaaca
     4321 gttctacgga atcgaggtgg aacatggctt cctgggcggt gatctgcgcg aattccgaaa
     4381 gcggcaattt gttcaggacc gtttgcatgg ccgattcgtt ctcggcgcgc aggacgatcc
     4441 acaactcccg gcggtcgccg gtgagggagt aggtgcgaat cacgccctgg cgcaggagcc
     4501 ggtctacgat cgcccgttgt gccgggatga gttggaggaa ttcctgcgtt gggaatgccg
     4561 ggaagcggag gcggatcatc cagtggttca tcgttctttg agctaaaaaa accggttcgt
     4621 ttcggaaccg gtttttcgtt gaggttattg tttgatcaac accgcgtcga tcgtgaactc
     4681 gacctcatct ccgacgactg cttcgccggt tgcggcagcg ttggcgtatt tgagaccgaa
     4741 gtctttgcga ttgatcttac cggttgcttt gaaaccggct ttcagattgc cccacggatc
     4801 ttttacttgt ccgccataga ccagcggaac ttctactttc ttggtgacgt cacgtaccgt
     4861 caggttaccg ctcagtacat atttgttgtt accaaggctc ttgatgccgg tgctggtgaa
     4921 cgtcatggtc gggaatttct ccgcgttgaa gaaatcgtca ctgagcaggt gcttatcgcg
     4981 catctcgtta tcggtggtga tgctgggaac ctgtaccgtc atcgttgcct ggaggtcgga
     5041 ccagtcggct ttcgaagagg taaaggtgcc ttcgaacttc ttgaattcac catcgacgtc
     5101 ggaaatgacg aggtacttcg tggtgaaacg gatcttggag tgggagccat ctagcttcca
     5161 gttctgcgcg attgcggtag cgctcagggc tacggccgcg atcagggtga ggactttttt
     5221 catcgggtta tgtttttggg gtttgattga tttcgtgcta caaatgtacg ttgaaacatt
     5281 agatgttaca acatataata ttcaaaatgt atgtaacgca ttcattatca ataacaaaaa
     5341 atcgagtaaa ttgttttaga aatgccggat tccgatggca tgccgggctt cccgcaccgt
     5401 tttgccggcg ctttcgcgtg ctttttcggc tccgtgtcga atgactttac ttagtcgagc
     5461 ttcgtcggaa agcatctccc ggatcttttc gcggatggga gcggtgaacg cgatcatgtc
     5521 ttcggccagt tgctttttca gatcgccgta ccggatcgcc atattattat aggcatcgtt
     5581 gaagtgcgca accgtatcgg gcgatgatac cacttcgagc agggtgaaga tgttccggat
     5641 cgcttccggt ttttcctggt tgggttgcgt aggtcccgaa tcggtcacgg cacgcatcac
     5701 tttcttgcgg atcacttccg gttcgtcgta taggaagatc gcgtttcctt cgccttcact
     5761 cttgcccatt ttcccgctgc cgtcgagtcc ggggatcttg accagttggg tgccgtagtt
     5821 gaacgcgtag ggctccggga aaagttcaca atggtaaagt cggttgaagc ggttgccgaa
     5881 ggtgcgtgcc atctcgaggt gttgttcctg gtcctttccg accggcactt tcaccgcgcg
     5941 gtggatgagg atatcggccg ccatgagcac cggataggtg agcagtccgg cgttgatgtt
     6001 gtcgggctgc ttgcggatct tctctttgaa ggacgtgcag cgctccaact cgcctacata
     6061 ggcgttcatg cccagcagca ggtacaactc ggcgatttcc gggaggtcgc tctgcacgta
     6121 aagcgtcgat acttcgggat ccagtccgca agccaggtac tcggccacga cctgccgaac
     6181 gttggcatgc aggtccgacg gggtcgggtg ggtggtcagg gaatggtaat cggcaatgaa
     6241 gaaatagcag ttatactcat gctgcatctt cagaaaattg ctgatcgcac cgaaatagtt
     6301 tcccaggtgc aggttgcctg tactccggat gccgctgacg acggtttctt tcataagggc
     6361 acaaagatag agaattgcag ggctccggcg ggtcgggcgg aattcgtcag agaaggagat
     6421 tctgttccag gtccctgctt ttttctgcga gcgatctcca gaagacggcg tgttcaggaa
     6481 atagtcgtga atagcgggcc agattcgctt cgttcatgcg gaacggaaac gagtaaacgg
     6541 ggatcttagc ttgtccattg cgccgggacc agatcgcaag caggtagatt cgctcgataa
     6601 ccggatcggt catcggcatg catccgatcg tcacgcagga gccgtgaatg aaaatatcgc
     6661 ctccgggatc cagtcttcct gcgcgttgcc ggtcctccgc atttggataa ttcaacccga
     6721 ggctcaggtg aaaattgctg tacggattga accggtcgag gtgataaacg ccttccggta
     6781 cctgaccgtc gcctttccgc tttttcggac cgagcattcc ggaacttgca cagatgggaa
     6841 acgttgcgaa gcggcgatag cgcttctcag aggttcgtcg cacatatacc tccagttcct
     6901 gctcggcttt ataggcaacg atcaagagct ggacatcatt cggcccgatt ccgaacttat
     6961 tcaggtcgtc ggtgagggtg ctgcggtgta ctttaatcgc atttgctaca cggggagcac
     7021 gaagttggtc ttgataaaaa tcaaccggag tggcagcagt taagtaaagg gatagtagca
     7081 ggaacgcaat aaaacgaagt tggatcatgg cgggtttagt ttgctgcatc acgattgagc
     7141 agaacatgac acgattgatg tcgacctgaa caacgaattt cgcccactcc aaattttcac
     7201 ccgggagtaa aatgggccgg gtaactgtct acggctttgt aaattgcacg cggtttcgaa
     7261 acctgtatca
//
LOCUS       DAOTON010000027         7258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_8991_length_7258_cov_3.216160, whole genome shotgun sequence.
ACCESSION   DAOTON010000027 DAOTON010000000
VERSION     DAOTON010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8991_length_7258_cov_3.216160"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1460)
                     /locus_tag="P5292_01025"
     CDS             complement(39..1460)
                     /locus_tag="P5292_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRS37736.1"
                     /translation="MIIEQIYTGCLAQGAYYIESDGEAAIIDPLREVHPYLQRAEKNG
                     AAIKYIFETHFHADFVSGHLDLSRKTGAPIIYGPNANPEFDAIIASDSQEFQLGRIRI
                     KVLHTPGHTMESSCYLLFDESGKPHSIFTGDTLFLGDVGRPDLAQKAADMTQEQLAGM
                     LYDSLYSKILPLPDDLTVYPAHGAGSACGKNMMKETVDSLGNQKKMNYALNQPSREAF
                     IAAVTDGLLPPPAYFPMNVALNKKGYQSVEGVINRGMQALSVAEFEHLAAGGDVLLLD
                     TRTSGNFASAFIPRSINIALDGQFAPWVGALIQDVDQAILLITDPGKEEESVIRLARV
                     GFDNVLGHLGGGIDSWIKAGKEVDKVHRISPAEFAAEVKIGKDVVIDVRREGEYRAEH
                     LEEAYNKPLDFINDWFRDIAEQGHFFLHCQGGYRSMTAASILKSRGIHDFTEVGGGFA
                     AIASTTSLPRTDYVCQSKLQPVK"
     gene            complement(1749..2549)
                     /locus_tag="P5292_01030"
     CDS             complement(1749..2549)
                     /locus_tag="P5292_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRS37737.1"
                     /translation="MTLELLGYAGAILMGLSLGLIGGGGSILTVPILVYLFSVDAVLA
                     TAYSLFVVGLTSLIGSFSHMRLGNIHWRTAIVFGIPSIISVYLTRAYVVPAIPDPVFH
                     LGDTAVSKAHAILLLFAVIMLLASYSMIRKRVVMENEEAEPRYNYPLILLEGIVVGAV
                     TGLVGAGGGFLIIPALVLLAKLPMKQAVGTSLIIIAAKSLIGFTGDLKGNQVIDWRFL
                     LIFSLIAMVGIIAGSMLSRNISNEKLKPAFGWFVLVMGVYIIAKEMMG"
     gene            complement(2559..2867)
                     /locus_tag="P5292_01035"
     CDS             complement(2559..2867)
                     /locus_tag="P5292_01035"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRS37738.1"
                     /translation="MKTLEERVNPEKLNKAAEMLRVAAHPQRLAILDLLRSGKHYNNS
                     ELQELLGIEQPILSQHLTLMRDKGLLNCTKEGKFAHYSIRYEDFHFIIDCIENCCTKL
                     "
     gene            3100..3762
                     /locus_tag="P5292_01040"
     CDS             3100..3762
                     /locus_tag="P5292_01040"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HRS37739.1"
                     /translation="MTVSIMQPYLLPYAGYYRLLKAEVFVIYDCVQFIRRGRIHRNEL
                     LNRNGQYDWFTLPIEKPGFHDRIDSVRFVTDWESELRSRAQAFPSFATAIERFPSLNC
                     LFEVRDLDLTDYLERQLIATRDLLGLPARIIRSSSLNIPESLKGQDRILEICRQLNAT
                     DYVNVPGGVTLYQPEVFRGAGIELHILSDYKLGYESMLDLLATKAPEDIRTAIDTTYR
                     FI"
     gene            complement(3781..4851)
                     /gene="arsB"
                     /locus_tag="P5292_01045"
     CDS             complement(3781..4851)
                     /gene="arsB"
                     /locus_tag="P5292_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRS37740.1"
                     /translation="MSADNCAPAHQRKRLGFLDRYLTLWIFLAMVVGIGLGHFVPALP
                     ASIHLLSSGSVDLPIAIGLILMMYPPFTKVQYEKLPSVFNRPKVLGASLLLNWVIGPV
                     LMFLLAILFLPDKPAYMTGLILIGLARCIAMVIVWNELAEGNREYAAGLVALNSIFQV
                     VFFSVYAWLFISVLPPFFGLAGAAIDISIAEIGKSVLIYLGLPFAAGYLTRRVLLAAK
                     GADWYQEKFIPRISPLTLIALLFTIVVMFSLKGDTIVQIPLDVLRVAIPLSIYFALMF
                     LISFLIGKKLGADYSVSTSIAFTAAGNNFELAIAVAIAVFGIHSGQAFAGVIGPLVEV
                     PALIALVNVAFWIRKHYYPTKS"
     gene            complement(4899..5339)
                     /locus_tag="P5292_01050"
     CDS             complement(4899..5339)
                     /locus_tag="P5292_01050"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRS37741.1"
                     /translation="MNPINVLFLCIHNSARSQMAEAYLKKFGGDAYHVESAGLEAGRL
                     NPLAVAVMQEDGIDISHNKTKDVFEFFQEGRRYDYVITVCDEASASRCPIFPGMHTKI
                     HWGFDDPSSFTGEEHERLARTRAVRDTIRTAVEEFVRNTRSKAR"
     gene            complement(5362..6204)
                     /locus_tag="P5292_01055"
     CDS             complement(5362..6204)
                     /locus_tag="P5292_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRS37742.1"
                     /translation="METADQLKKLVKAQYTEIAEQDKASNQSSCCGSGCCSDEVYNIM
                     SEDYAGLKGYNPDADLGLGCGLPTQFARIRKGDTVLDLGSGAGNDCFIARAETGDTGK
                     VIGVDMTEAMIAKARANAEKLGLNNVEFRLGEIEALPVGVGTIDVVVSNCVLNLVPDK
                     KKAFSEINRVLKPGGHFSISDIVLVGELPDSILKTAELYAGCVAGAIQKDAYLGMVRE
                     AGFENITLQKEKAISIPDDILAHYLSPEQLEEFKHSGTGIYSITVYAEKKKDACCDKD
                     TCCK"
     gene            complement(6254..6583)
                     /locus_tag="P5292_01060"
     CDS             complement(6254..6583)
                     /locus_tag="P5292_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRS37743.1"
                     /translation="MGLAKIDEFTLLENRIAKYAKALGHPARVAILRLLLKRQACVCG
                     DIVDELPLSQSTVSQHLKELKNAGLIKGEIEGVRTCYCIDEAEWDKAKKCLVELMQTP
                     LTNIRCC"
     gene            complement(6802..6895)
                     /locus_tag="P5292_01065"
     tRNA            complement(6802..6895)
                     /locus_tag="P5292_01065"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(7033..7242)
                     /locus_tag="P5292_01070"
     CDS             complement(7033..7242)
                     /locus_tag="P5292_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRS37744.1"
                     /translation="MYYKGYSTNLEIRLQQHNSDESRFTAGKGPWTLVYFEQFESKAD
                     ALKREKVLKKLNRTSILKLIGSTAG"
BASE COUNT         1629 a         1921 c         1991 g         1717 t
ORIGIN      
        1 ccccggatgg ggaggctgaa tcatgggttg tggttggatt attttacagg ttggagcttc
       61 gactgacaga catagtccgt gcgcggcagg gacgtggtgc tcgcgatcgc ggcaaatcca
      121 ccgcctactt cagtgaagtc gtggatgcca cgtgacttca ggatgctggc agcggtcatg
      181 ctgcggtagc ctccctggca gtgcaggaag aaatgtcctt gctcggcaat gtcacggaac
      241 cagtcgttga tgaagtccaa cggtttgttg taagcttctt ccagatgctc ggcacggtat
      301 tctccttcgc gacggacgtc aataacgacg tccttgccga tttttacttc ggcggcgaat
      361 tctgccgggc tgatccgatg aaccttgtcg acttctttcc ccgctttgat ccaactgtca
      421 ataccgccgc ccaggtggcc gagtacgtta tcgaaaccga cgcgcgcgag ccggatgacc
      481 gattcctctt ccttacccgg atcggtgatc aacagaatgg cctggtccac gtcctgaatg
      541 agtgcgccta cccagggagc gaattgcccg tccagtgcga tgttgatcga gcgcgggatg
      601 aaagctgatg cgaaatttcc agaagtacgg gtgtcgagta acaagacatc gccacctgcc
      661 gcgaggtgtt cgaattcggc tacgctcaac gcctgcatgc cgcggttgat gacgccttct
      721 accgattgat aacctttctt gttcaaggcg acattcatcg ggaagtaagc cggcgggggc
      781 agcaaaccgt cggtaacggc tgcgatgaac gcttcgcgac tcggttggtt gagcgcgtag
      841 ttcattttct tctggttgcc gagcgagtcc accgtctcct tcatcatgtt cttgccgcag
      901 gcgctgccgg caccgtgtgc aggatagacc gtcagatcat ccggcaaggg aaggattttg
      961 gaatacaggg aatcatagag catgcctgca agctgctcct gggtcatatc ggcggctttc
     1021 tgcgcaaggt caggacgtcc gacatcgccg aggaacaagg tgtcgccggt gaagatactg
     1081 tgcggcttgc cgctctcgtc gaagaggagg tagcaactgc tttccatcgt gtggccgggc
     1141 gtatgcagca ccttgatgcg gatgcggccc aattggaatt cctggctgtc actcgcaatg
     1201 atggcatcga actcggggtt cgcgttcgga ccataaatga tcggagcacc ggtcttacga
     1261 ctcaggtcga ggtgacccga aacaaagtcg gcatggaagt gcgtttcaaa gatgtacttg
     1321 atcgccgcgc cgtttttctc ggcccgctgt aagtaaggat gcacttcccg caacggatca
     1381 atgatggccg cttcgccatc gctctcaatg taatacgcgc cctgcgcgag acaaccggtg
     1441 tagatctgtt cgatgatcat gaggaggggt attgttgttt gttgtttgtt gtttcgagtt
     1501 ccctgcctac gctaaagctt cggcaggcag gcgagttccg tgttctcaag cttcagaaga
     1561 aagagaaagg gaccgcttca ttttttcact attcactatt cgctattcac tgttcactcc
     1621 cgcctacact aaagctccgg cgggcaagat tcaccattta ctattcgcta ttcgctattc
     1681 gctattcgct attcgctatt cactatttac tgttcactgt tcactattca ctattcacta
     1741 ttcactcctc accccatcat ctccttcgca ataatataaa cccccatcac cagcacgaac
     1801 cagccgaatg ccggcttgag tttttcgttg ctgatgtttc gggacaacat actgccggcg
     1861 atgatgccga ccatggcaat gagtgagaag ataagtaaaa atcgccaatc gattacctga
     1921 ttgcctttga ggtcgccggt gaaaccgatc agtgattttg cagcaataat gatgagcgag
     1981 gtgccaacgg cttgtttcat gggcaatttc gcaaggagta cgagggccgg gatgatgagg
     2041 aaaccgccac ccgcaccgac cagaccggtc accgcgccga ccacgatgcc ttccagcagg
     2101 atcaggggat aattgtagcg cggttccgct tcttcgtttt ccatgaccac ccgcttgcgg
     2161 atcatcgaat acgaggcgag cagcatgatg acggcaaaca agagtaggat cgcatgtgct
     2221 ttactgactg ccgtgtcgcc cagatgaaag acggggtccg ggatggccgg tacgacatag
     2281 gcgcgcgtca ggtaaacgct gatgatcgaa ggaattccga atacgatagc ggtacgccag
     2341 tggatgttcc caagccgcat gtgactgaaa gagccgatca agcttgtcag gcccacaaca
     2401 aagagcgagt aggcggtcgc gagtacggca tcgaccgaga agaggtaaac caggatcggg
     2461 accgtgagta tggagccacc accgccgatc aagccgaggg atagtcccat caggatcgct
     2521 cctgcataac ccagtaattc aagcgtcata agcgtaggtc agagttttgt gcagcagttt
     2581 tcgatgcagt cgatgatgaa atggaaatcc tcgtaacgga tggagtagtg ggcgaacttt
     2641 ccttctttgg tacagttcag caatcccttg tcacgcatca acgtaaggtg ctgcgacagg
     2701 atcggttgtt caatgccgag cagctcctgc aattcggagt tgttgtagtg cttgccgctc
     2761 cgcaacaaat ccagaatggc caatcgttgt gggtgagcgg caacccgaag catctctgcc
     2821 gctttattga gtttttcggg attcacccgt tcctcgagcg ttttcatggt gttgaacttg
     2881 ggcacaaaga tacatatagt tatataacta tgtgacaatg ttaacgtatc tatttcctat
     2941 atttttgtat atcagctaaa tagattttgg tcgatgcccg gaggaaggat gttttctaat
     3001 ccccggacga caattgccac taaataaatc aggcgttggg agtatacttt ttcccggaat
     3061 ttccgttgaa aaaggctgtt tcaagatcct gcccacccca tgaccgtcag catcatgcaa
     3121 ccttacctgc taccgtatgc aggctattac cggctgctga aggcggaggt gttcgtcatc
     3181 tacgattgtg tgcagttcat ccgccgtggt cgcattcacc ggaatgagct gctgaaccgg
     3241 aacggtcagt acgactggtt caccctgccc atcgaaaagc ccggctttca cgatcgtatc
     3301 gattccgtga ggttcgtcac tgattgggaa tcggaattgc gttcgcgcgc tcaggctttc
     3361 ccgtccttcg caacggcgat cgaaaggttc ccttccttga attgtctgtt cgaagtgcgc
     3421 gatctcgacc tgaccgatta tctcgaacgc caactcatcg cgacaaggga cctgctcggt
     3481 ttacctgcgc ggatcattcg ctcgtcttct ctcaacattc cggaatcgct gaaaggccag
     3541 gaccgtatcc tggaaatttg ccgacaacta aacgcgacgg actatgtcaa cgtccccggc
     3601 ggtgtgacgc tttaccagcc cgaggtattt cgcggcgcgg gcatcgagct gcacattctc
     3661 tccgactaca agctcggtta cgagagcatg ctcgatttgc tggccacaaa ggcgccggaa
     3721 gatatccgga cggcgatcga taccacctat aggtttatct gaacaccgat cccgccgcgc
     3781 tcaggatttg gtcgggtaat agtgcttccg gatccagaac gccacgttca ccaacgcgat
     3841 cagcgcgggt acttccacca gcggaccgat cacaccggca aaggcctgtc cgctatggat
     3901 accgaatacg gcaatggcca ccgcgatcgc cagttcgaaa ttgttgcctg cggccgtgaa
     3961 ggcgatggag gtactcacgg aataatccgc cccgagcttt ttgccgatca ggaaactgat
     4021 gaggaacatc aaggcgaagt aaatgctcaa ggggatcgcc acccgcagca cgtcgagcgg
     4081 gatctgcaca atggtatcgc ccttaagact gaacatcacc acgatggtaa acaagagtgc
     4141 gatcagggta aggggcgaga tgcgtggaat gaatttttcc tggtaccagt ctgctccttt
     4201 agccgcgagc aggacgcggc gggtcagata accggccgcg aagggaagac cgaggtagat
     4261 caatacgctc tttccgattt ctgcgatgga gatgtcgatc gctgctcccg ctaagccgaa
     4321 gaaggggggc aggacggaga tgaacaacca ggcgtacacg ctgaagaaca cgacctggaa
     4381 aatgctgttc aacgcgacca ggccggccgc gtattcccgg ttcccttccg ccagttcgtt
     4441 ccagacgatc accatcgcga tgcaacgtgc caggccgatc agaatcaggc cggtcatgta
     4501 cgcgggtttg tccggcagga agaggatcgc gagcaggaac atcaacaccg ggccgatcac
     4561 ccagttgagg aggagggaag cgcccaacac cttcggccgg ttgaagaccg acggtagctt
     4621 ttcgtattgc actttggtaa agggcggata catcatgagg atcagcccga tggcaatcgg
     4681 caggtcgacg ctgcccgaag aaagcaagtg gatgctggca ggcaaggccg gaacaaaatg
     4741 gccaagtccg atgccgacta ccatggccag gaagatccag agggtcaggt aacggtcaag
     4801 gaatccaaga cgcttgcgtt gatgggcggg agcacagttg tcagctgaca tgtgataaag
     4861 gaagttgttg aacgaagact ggttggacgg caggaaactc accgggcttt tgaccgcgtg
     4921 ttgcgaacga attcttccac ggctgtccgg atcgtatcgc gcaccgctcg tgtgcgggcc
     4981 agtcgctcgt gttcttcacc ggtgaaagag gaaggatcgt cgaatcccca atggatcttc
     5041 gtatgcatgc ccggaaagat cgggcaacgc gaagcgctgg cttcatcgca gaccgtgatc
     5101 acatagtcgt accgacgacc ctcctggaag aactcgaaaa cgtctttcgt cttgttgtgt
     5161 gagatgtcga tcccgtcttc ctgcatgacc gctacggcca gaggattgag tcggccggcc
     5221 tccaatccgg cgctttctac gtggtaggca tcgcctccga attttttcag ataggcttcc
     5281 gccatctggc tgcgggcgga gttgtgaata caaaggaaga ggacgttgat cgggttcatg
     5341 gtgaagggga tgagcgggcg gttatttaca acaggtgtct ttgtcacaac aggcgtcttt
     5401 tttcttttca gcatacacgg tgatgctgta gatgccggtg ccgctgtgct tgaattcctc
     5461 cagttgctcc gggctcaggt agtgcgcgag gatatcgtcg gggatggaga tcgccttttc
     5521 tttttggagg gtgatgtttt cgaatccggc ttcccgcacc atgccgaggt acgcgtcttt
     5581 ctggatggcg ccggccacgc agcctgcata cagctcggcg gtcttgagga tcgaatccgg
     5641 gagctcgccc acgaggacaa tgtcggagat ggaaaaatgt ccgccgggct tcagcacccg
     5701 gttgatttcc gaaaaggctt ttttcttgtc gggcaccagg ttgagcacgc agttgctgac
     5761 caccacatcg atcgtgccca cgcctacggg cagtgcttcg atctcgccca ggcggaactc
     5821 gacattgttc aggcccagct tttcggcgtt cgctcgtgcc ttggcgatca tggcctcggt
     5881 catgtcaacg ccgatcacct tgccggtatc gcccgtttcc gcgcgcgcga tgaaacaatc
     5941 gttgcccgcg ccggaaccca ggtccaggac ggtatcgcct ttccggatcc tggcgaattg
     6001 cgtcggcaga ccacagccca gtccgagatc cgcgtccggg ttatagccct tcaatccggc
     6061 atagtcttcc gacatgatgt tatagacctc gtccgagcag cagcccgaac cgcagcagga
     6121 ggactggttg gaagctttgt cctgttcggc gatctcggtg tattgcgctt tcaccagttt
     6181 tttgagttga tcggcagttt ccatagtggt tgttttatat cgtattatta cgatgaatta
     6241 gggcaaattt tttttagcag caacggatat tggtcagcgg cgtctgcatc agttcaacca
     6301 ggcatttttt ggccttatcc cattcggctt cgtcgatgca gtaacaggtg cgcacccctt
     6361 cgatctcgcc tttgatgagt ccggcgttct tgagttcttt caggtgttgc gaaaccgtcg
     6421 actgggaaag cggcagctcg tccacgatat ccccgcagac acaggcttgc cgcttcaaca
     6481 gcaaacgcag gatcgccacc cgggcaggat gccccagcgc tttcgcgtat ttcgcgatgc
     6541 ggttctccag cagggtgaat tcatcgatct tggcgaggcc catggcgtta attatagatc
     6601 gcaatattac gattaatgat ggtcggttgt caaggacttt aacatacttt tcgatgtaag
     6661 cctgaaaacg aaccggaaat tgcgtctgga tcccgaactc caaaaagaaa attcgaatga
     6721 acgagatgga cctagcctga ctcggggcta cgctaacgga tgaaaaagaa aaaggaagtc
     6781 aaacttgact tccttttatt ctggcggtcc ggacgggact cgaacccgtc tcgtcactag
     6841 tgacgagatg acaggcatgt tcccgatagc tatcgggact aaccggctga actacttatt
     6901 taattgtgct gccatcttcg atggcctaaa aagcaaagag ccatcagttt cctgatggct
     6961 ccttagcggt ccggacggga ctcgaaccca tctcgtctcg tccaaagacg agacgagatg
     7021 acaggcatgt tatcatccag ccgtactacc gataagctta aggatcgatg ttctgtttaa
     7081 tttttttaaa accttctccc gctttagtgc atcagccttg ctttcaaatt gttcaaaata
     7141 aacgagcgtc caaggcccct taccagctgt gaacctcgat tcatctgaat tgtgctgttg
     7201 aagcctgatc tcaaggttag ttgaataacc tttgtagtac acatcagcgc tgctgcta
//
LOCUS       DAOTON010000028         7246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_9001_length_7246_cov_3.248783, whole genome shotgun sequence.
ACCESSION   DAOTON010000028 DAOTON010000000
VERSION     DAOTON010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9001_length_7246_cov_3.248783"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="P5292_01075"
     CDS             complement(<1..1343)
                     /locus_tag="P5292_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HRS37745.1"
                     /translation="MIKRVLAVFVSALCRSLQLQAQAPAPSAATVRPLLADYLVQHQQ
                     DWQLSDSDLNDFVVSDCYTDAKTGQTYAYLHQTVGGIRIFNAVSAASIREGRITGFGK
                     KLYTNAAARVNSLQPTLSAAAAVQRAAAHLGKAIVGDPRELASDPAMHRHYFGASGIA
                     RRPIRVDLVFVPVKNALRLAWDVNIDLEGEAHWWNVRIDALSGDFLEKNDWTVHCDFG
                     TPDAAQVQRQQHQHAGVQEHVATSVSTQRSSSLPVYNVLALPVEAPSFGARTLVTDPA
                     DPAASPYGWHDVDGAVGAEYTITRGNNVYAYDDINDQDVPGTSPDGGASLNFDFPINF
                     TQQPGTYLEASVTNLFYTNNWMHDVLWHHGFDGPSGAFQENNYGTAGADGDPVMAECQ
                     DGGGTNNANFATPDDGQSGRMQMYLWSGSPDIDGSLDNGVIMHELDSKGLLKREAL"
     gene            complement(1643..2530)
                     /gene="atpG"
                     /locus_tag="P5292_01080"
     CDS             complement(1643..2530)
                     /gene="atpG"
                     /locus_tag="P5292_01080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRS37746.1"
                     /translation="MANLKEVRNRIASVNSTQQITKAMKMVSAAKLRRAQDAILTMRP
                     YAAKLREIMENISGTLEGGAGGDFARVRPVEKVLIVAVNSNRGLCGAFNANINRAVNR
                     LIRETYAKQAKEGNVKVLAIGKKASDFFSKQKNVLVGNHNEVYANLNFENVSAIAEKI
                     MQEFAAGQYDRVEVVYNQFKNAAVQYVITEQMLPIAPPATKGDAAKTAASDYIFEPRK
                     EELVLELIPKSVKVQLFKAVLDSHASEHGARMTAMNKATDNAGEMLRELRLSYNKARQ
                     AAITGEILEIVAGAEALKG"
     gene            complement(2679..4259)
                     /gene="atpA"
                     /locus_tag="P5292_01085"
     CDS             complement(2679..4259)
                     /gene="atpA"
                     /locus_tag="P5292_01085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRS37747.1"
                     /translation="MAEVRPDEVSAILRQQLAGFKTEKELEEVGTVLQVGDGIARIYG
                     LTKVQSGELIEFENGLRGVVLNLEEDNVGAVLLGKSTDIREGDVVKRTGQIASIKVGD
                     GMVGRVVDTLGTPIDGKGPISGELFEMPLERKAPGVIYRQPVNEPLQTGIKAVDAMIP
                     IGRGQRELIIGDRQTGKTAVAIDTIINQKEFYERGEPVYCIYVAVGQKGSTVAQIVKT
                     LTDNGAMPYTIIVSATASDPAPMQFFAPMAGAAIGEYFRDTGRPALIVYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKVINSDEIARQMNDLPETLKNKVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLEANLFNSGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQYRELEAFSKFGSDLDAATKATLDKGSRNVEILKQGQY
                     NPMSVEKQIAIIYLGTRNLLRDVPTNRVKEFETEYLALLDAQHKATLDSLRKGVIDDS
                     VTSVLEKAAASLTPKYKA"
     gene            complement(4305..4862)
                     /gene="atpH"
                     /locus_tag="P5292_01090"
     CDS             complement(4305..4862)
                     /gene="atpH"
                     /locus_tag="P5292_01090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRS37748.1"
                     /translation="MHNIKVAKRYAKSLIDLAKETGVLDAVSADMKLLVSVCQSSKEL
                     TSLLGNPIINSDKKEAVLNAIFGDKIQPMTRSFFQILIRKGREGDLYEIAKEYIAQYK
                     QLKGITTAVVITATGLNDHLRSEVNRIVKEKVQREVELLEQVDKDLIGGFVLRIGDTQ
                     YDASVVSQLRRLAKEFSSNPYVRKN"
     gene            complement(4879..5373)
                     /locus_tag="P5292_01095"
     CDS             complement(4879..5373)
                     /locus_tag="P5292_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRS37749.1"
                     /translation="MELIQPAIGLIFWMSVSFLLLLFILRKFAWKPILKMLSDRETSI
                     AEALNSARKAKDEMANLKADNERLLNEARAERDRMLKEARDTKDAIVAEAKSKAQTEA
                     NKVLQQARETIQTEKLAAITELKNQVATMSIEIAEKILRTELGNDEKQKALVQSLLKD
                     INLN"
     gene            complement(5463..5717)
                     /gene="atpE"
                     /locus_tag="P5292_01100"
     CDS             complement(5463..5717)
                     /gene="atpE"
                     /locus_tag="P5292_01100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRS37750.1"
                     /translation="MLSSILLEAGNLGLGYGVAALGAGLAALGAGVGIGRIGGSALES
                     IARQPEASGDIRANMILTAALVEGAAFFAMVVGLLVVFKS"
     gene            complement(5747..7021)
                     /gene="atpB"
                     /locus_tag="P5292_01105"
     CDS             complement(5747..7021)
                     /gene="atpB"
                     /locus_tag="P5292_01105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRS37751.1"
                     /translation="MSQRVHYKSISSRLAAALCLLYFVFGLPVVRANQAEQEVGMHTD
                     SAAAEMITEATAADHSAASEEHAQGEEKFDAGKMIVDHITDAHDWHLWGHGHSAVSVP
                     LPVILKSEQGLHFFMSSAFHHDNDGKHVVDHNGERFVRYHNRIYIAGTSADAEGRFVS
                     VNEKGEASNVAPLDFSITKNVAALLFSVILLLWIFLSVAKAYRRREGQAPKGLQSLIE
                     PLIIFVRDEIAKPSIGAKHMKYMPYLLTVFFFIWINNLLGLIPIIPGGANLTGNIAVT
                     MTLALITFIITTVSANKHYWRHVFAMPGVPVGVLVLLTPIELLGVFLRPFVLMIRLFA
                     NITAGHIIALSFFSLIFIFGEMSTGLGLGVSVFSVAFVVFMSLLELLVAFLQAFVFTL
                     LSAIYFGSAVEEHDHHHEDEHGHAHVEEAAIV"
BASE COUNT         1484 a         1928 c         2195 g         1639 t
ORIGIN      
        1 agcgcctcgc gcttgagcag gcccttgctg tccagttcat gcatgatgac gccgttatcc
       61 agcgagccgt cgatatcggg cgatccgctc cagagataca tctgcatgcg accgctttgg
      121 ccgtcgtcgg gtgtcgcgaa gttggcgttg ttggtaccgc ctccgtcctg acactcggcc
      181 atcaccggat ctccgtcggc tcctgccgtg ccgtagttat tctcctggaa cgcgccggaa
      241 ggaccgtcga agccgtggtg ccaaagcaca tcgtgcatcc agttgttggt atagaagagg
      301 ttcgtgacag aagcttcgag atacgttccc ggttgctggg taaagttgat cgggaagtcg
      361 aagttcaggg atgcgccacc gtccggcgag gtgccgggta cgtcctgatc gttgatgtcg
      421 tcataggcgt aaacattgtt accacgcgtg atggtgtatt ccgcgccgac cgcgccgtca
      481 acgtcatgcc atccgtatgg ggaggcggcc ggatcagccg gatcggttac cagcgtacgt
      541 gcaccgaagg aaggtgcttc aaccggcaat gccaggacat tatacaccgg cagcgagctg
      601 ctgcgctggg tgctgacaga cgtcgcaaca tgctcttgca cgccggcatg ttgatgctgc
      661 tggcgctgaa cctgtgcagc gtccggtgta ccgaagtcgc aatgtacggt ccagtcgttc
      721 ttctcaagaa aatcgcccga caaggcgtcg atgcgtacgt tccaccagtg tgcctctcct
      781 tcgaggtcga tgttcacatc ccaggcaagt cgcagggcat tcttcaccgg aacgaagacc
      841 agatcgaccc gaatgggacg acgggcaatc cctgacgccc cgaagtaatg gcggtgcata
      901 gcgggatcgg aagcaagctc acgcggatcg cctacgatcg ccttacccag gtgcgcggcg
      961 gcacgttgta cagctgcagc agcagatagt gtcggctgca gactattgac gcgcgcggcg
     1021 gcgttggtgt aaagtttctt tccgaagccg gtgatacggc cttcccggat gctggcggca
     1081 ctgaccgcgt tgaagatgcg gataccacct accgtctggt gcaggtaggc ataggtctgt
     1141 ccggtcttcg catcagtgta acagtcactg acgacgaagt cgttcagatc ggaatcactg
     1201 agctgccaat cctgttggtg ttggacgaga taatcagcga gcagcgggcg aaccgttgcc
     1261 gcggaaggag ctggagcctg cgcctgcaat tgcagggacc gacaaagtgc cgacacgaag
     1321 acggcgagta cacgtttgat catgatgatg gttcagaata gtcgtgaggg aagaagcgcg
     1381 caggcgatat ccgagtgggt cgaaggacgg gacaggggaa acgctgttca gtgcagcttt
     1441 gcgcttgttc ctcaaatgta acgaacagat gtattcgtga caatgttgtc tgtgcaaggg
     1501 cgactgaaag acccgaaatc tgaatgtttc ctgacaatag cgggaaatta ggccctgatt
     1561 cgggaaaaat ccatcctccg ctttgaaaaa tgaaagcggc tgccccgttg ggagcagccg
     1621 cttctaagtc gcattggatg gatcaaccct tgagggcttc cgcgccggcg acgatctcga
     1681 ggatctctcc ggtaatggcc gcctgacggg ctttgttgta tgaaaggcga agttcacgga
     1741 gcatctcccc cgcgttatcg gtcgccttgt tcatggcagt catccgcgca ccgtgttcgg
     1801 aagcgtggga gtcgaggacg gctttgaaca attgcacctt caccgacttc gggatcaact
     1861 cgagtacgag ctcctccttg cgtggttcga agatgtaatc gctggcagcc gtctttgccg
     1921 catcgccctt agtagcggga ggcgcgatcg ggagcatctg ctcggtgatc acgtactgga
     1981 ccgccgcgtt cttgaactgg ttgtacacca cttccacgcg atcatactgc ccggccgcga
     2041 attcctgcat gatcttctcc gcaatggcgg atacgttttc aaagttcagg ttcgcgtaaa
     2101 cttcgttgtg attgccgacc aatacgttct tctgcttgga gaagaagtcg gaggcctttt
     2161 tgccgatggc cagcaccttc acattgcctt ctttagcctg cttcgcgtag gtctcgcgaa
     2221 tgagtcggtt gaccgcacgg ttgatgttgg cattgaaagc gccgcacaaa ccacgattgg
     2281 aattgacggc tacgatgagc accttctcca ccggtcgcac gcgcgcgaaa tcgccgcccg
     2341 caccgccttc gagcgtgccc gagatattct ccatgatctc gcgcagcttg gccgcgtacg
     2401 gacgcatggt caggatggcg tcctgcgcgc ggcggagctt ggccgcgctc accattttca
     2461 tcgccttggt gatctgctgc gtcgaattga cggaggcgat gcggttacgg acttctttca
     2521 gattggccat ggatggtggg ggttagtgaa tcgtaattag taattagtaa ctagaaactg
     2581 gtaattggtt attcgtgatt ggttattgga taaaaaacga cgaaaggacc gtccgcttct
     2641 acactactgt tcactgttta ctgttcactg ttcactaatc aggctttgta cttcggagta
     2701 agtgacgcag cagccttctc cagcaccgag gtcacgctgt cgtcgatgac gcctttgcgg
     2761 agcgaatcga gcgttgcttt gtgctgggcg tcgagcagtg cgaggtattc ggtttcgaat
     2821 tccttcacgc ggttcgtcgg aacgtcgcgg agcaggttgc gggtaccgag gtagatgatc
     2881 gcgatctgct tctctaccga catcggattg tactggccct gcttcaggat ctccacgttg
     2941 cgggaacctt tgtcgagcgt cgccttggtg gcggcgtcga ggtcggaacc gaacttggag
     3001 aaggcttcga gttcacggta ctgggcctgg tcgagcttca gcgtaccggc gactttcttc
     3061 atcgacttga tctgcgcgtt accgcctacg cgggatacgg agatacctac gttgatggcc
     3121 ggacgtacac cggagttgaa gaggttcgct tcgaggaaga tctgaccgtc ggtgatcgag
     3181 atcacgttcg tcgggatata cgcggatacg tcaccggcct gggtctcgat gatcggaagc
     3241 gcggtgagcg agcctccgcc tttcaccttg ttcttcagcg tctcgggcag gtcgttcatc
     3301 tggcgagcga tctcgtcgga gttgatgacc ttggccgcac gctcgagcag acgggagtgc
     3361 aggtagaata cgtcacccgg atacgcttcg cggcccggag gacgacggag cagcagggac
     3421 acttcacggt aggcgacggc ctgcttggac aagtcatcgt atacgatgag cgccgggcgg
     3481 ccggtatcgc ggaaatattc gccgatagcc gcgccggcca tcggagcgaa gaactgcatc
     3541 ggcgccggat cggaagcggt agccgacacg atgatggtgt acggcatggc gccgttgtcg
     3601 gtcagcgtct ttacgatctg cgcgacggtg gagcctttct gacctacggc aacgtagata
     3661 cagtaaaccg gttctccgcg ctcgtagaat tctttctggt tgatgatggt atcgatggcc
     3721 acggcggtct tacccgtctg acggtcaccg atgatcagtt cgcgctgacc gcgaccgatc
     3781 ggaatcatgg cgtcaacggc cttgatgccg gtctgcaacg gttcgttcac cggctgacgg
     3841 tagattacac ccggcgcttt gcgctcgagc ggcatctcga acagctcacc gctgatcggg
     3901 cctttgccgt cgatcggggt gcccagcgtg tcgacgacac ggccgaccat tccgtcaccc
     3961 accttgatgg aggcgatctg accggtacgc ttcacgacgt cgccttcgcg gatgtcggtg
     4021 gatttaccga gcaataccgc accgacgttg tcttcttcga gattcagtac gacgccgcgc
     4081 aggccgttct cgaattcgat gagttcaccg gactgcactt tggtgaggcc gtagatgcgg
     4141 gcgataccgt cgcccacctg gagcacggtg cctacttctt ccagttcttt ctcggtcttg
     4201 aagccggcca actgctgtcg cagtatcgcg gagacttcgt cgggtcttac ttctgccatg
     4261 atggtatgat gttgtcttgt tgcttatgga taggtgtccg gagatcagtt cttgcggacg
     4321 tatgggttcg agctgaattc ttttgccagg cggcggagct ggctcacgac gctggcatcg
     4381 tactgtgtat cgccgatgcg caacacgaaa ccgccgatca ggtccttgtc gacctgctcg
     4441 aggagttcca cttcgcgctg tactttttcc ttcacgatgc ggttcacctc cgagcgcagg
     4501 tggtcgttga ggccggtggc ggtgatcacg acggcggtcg tgatgccctt gagttgcttg
     4561 tattgggcga tgtattcttt cgcgatctcg tacaggtcgc cttcgcggcc cttccggatc
     4621 aggatctgaa agaacgaacg tgtcatcggc tggatcttgt cgccgaatat cgcgttcaat
     4681 accgcttcct tcttgtccga attgatgatc ggattgccga ggaggctggt gagttccttg
     4741 ctggactggc aaaccgaaac gaggagtttc atgtccgcac tcaccgcgtc caggactccg
     4801 gtctccttgg ccaggtcgat cagcgacttg gcgtaacgtt tcgcgacttt gatgttatgc
     4861 atgggatcag gagaccgatc agttcaggtt gatgtctttc aggaggctct gcacgagcgc
     4921 cttctgcttt tcgtcgttgc ccagctcggt gcgcaggatc ttttccgcga tctcgatcga
     4981 catggtggcc acctggttct tgagttcggt gatggcggcg agcttctcgg tctggatggt
     5041 ctcgcgggcc tgttgcaaca ccttgttggc ttcggtctgc gccttcgact tcgcttcagc
     5101 cacgatcgcg tccttggtat cgcgtgcttc tttcagcatg cgatcgcgtt cggcgcgggc
     5161 ttcgttgagc aggcgctcgt tgtcggcctt caggttggcc atctcgtctt tcgctttgcg
     5221 ggcggagttc agtgcttcgg cgatgctcgt ttcgcggtcg ctcagcatct tcaggatggg
     5281 cttccaggcg aacttgcgca ggatgaagag caacagcagg aaagaaacgc tcatccagaa
     5341 gatgagaccg atggcgggtt gaatgagttc catgttacag gaagtggttt ttcgtttttt
     5401 cgattctcga attaaacaaa cccggtgcgg ccaaccgccg caccggattc gttcgggcag
     5461 gatcagctct tgaacacgac gagcagacca acaaccatcg cgaagaaggc ggcgccttcc
     5521 acgagagctg cggtcaggat catgttggcg cggatgtcgc cggatgcttc aggctggcgg
     5581 gcgatggatt cgagtgccga accaccgata cgaccgatac cgacgccagc gccgagagcg
     5641 gcgaggccgg cacccaaagc agcaacaccg tagccgaggc caaggttacc tgcttccagg
     5701 agaattgaag ataacatagg gttgtttatt aagggttaat ggataatcag acaatagccg
     5761 cttcttccac gtgggcatgg ccgtgttcgt cttcgtgatg gtggtcgtgc tcctctacgg
     5821 cggaaccgaa atagatcgcc gagagcaggg tgaacacgaa cgcctgcagg aacgctacga
     5881 ggagttcgag caggctcatg aagacgacga acgcgacgga gaagaccgag acgcccaggc
     5941 cgaggccggt gctcatttct ccgaagatga agatgagact gaagaacgag agcgcgatga
     6001 tgtgacccgc cgtgatgttg gcgaaaagtc ggatcatcaa cacgaagggg cggaggaaga
     6061 caccgagcaa ttcgatcggc gtgagcagga cgagtacacc gaccggtacg ccgggcatgg
     6121 cgaagacgtg acgccagtag tgcttgttcg cgctgaccgt ggtgatgatg aaagtaatga
     6181 gcgcgagggt catcgtcacc gcgatgttac cggtcaggtt ggcaccaccc ggaatgatcg
     6241 ggatcagacc caggaggttg ttgatccaga tgaagaagaa caccgtcagc aggtacggca
     6301 tgtacttcat gtgcttggcg ccgatgctcg gcttcgcgat ttcgtcgcgt acgaagatga
     6361 tgagcggctc gatgagcgac tgcagccctt tgggcgcctg gccttcgcgg cggcggtagg
     6421 ctttggcgac ggacaggaag atccagagga gcaatatcac gctgaacagc agcgcggcga
     6481 cgttcttggt aatggaaaaa tcgagcggcg cgacattgga tgcttcgcct ttctcattca
     6541 ccgaaacaaa acgcccctcg gcatcggcgg aggtgccggc gatgtaaatg cggttgtggt
     6601 agcggacaaa acgttcgccg ttgtgatcca ctacgtgctt gccatcgtta tcgtgatgaa
     6661 aagcagagga catgaaaaag tgcagcccct gttcggattt taaaattacc ggcaggggaa
     6721 cggaaacagc ggaatgcccg tgaccccaga ggtgccagtc gtgcgcgtcg gtgatgtgat
     6781 cgacaatcat cttgccggca tcgaacttct cttcgccttg cgcatgctcc tctgaagcgg
     6841 cggaatgatc cgcagccgta gcttccgtaa tcatttcggc tgcggcagag tcggtatgca
     6901 taccaacttc ctgctcagcc tgattggcac gaacaaccgg aagaccgaaa acgaaataca
     6961 acaggcagag cgcagccgcc aacctggagc taatggactt ataatgaacg cgttgtgaca
     7021 tacccagcaa ccgtgatttt caaaagagag cgcaaaagta ctataaagag tggaaagggc
     7081 aaacgggaaa agttcgccaa gcgcttgtaa ttgtctgaaa atcagattat caggaataat
     7141 ttttggagcc ccgatttcaa tgcgaaataa tgctgagtcc tacttgtata tagaggtaaa
     7201 ggtagaccgc ctaagaaacg ttattttcgt tcgaaaaatc gtgttg
//
LOCUS       DAOTON010000029         7042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_9300_length_7042_cov_3.569343, whole genome shotgun sequence.
ACCESSION   DAOTON010000029 DAOTON010000000
VERSION     DAOTON010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9300_length_7042_cov_3.569343"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3527
                     /locus_tag="P5292_01110"
     CDS             <1..3527
                     /locus_tag="P5292_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS37752.1"
                     /translation="NDTLIIDAVYLSPEFGQITVPNVNANGMPGVSTLSSTSGNIALQ
                     QVQIIGQPGNVGYHTIFLEGTDNGTPAQTASAPIVIRVIPAPNSNFTVSPTGIQMPGT
                     LFTFTNTSVGGFSAVWDFGDGSPPSSLYNTSHIYGSGGTYTVTLTVTSPNGCTDVFTQ
                     QVQIQQCGTASFTTSNVCAGSPSLITFTGTAGVGATYNWNFAGGTVVSGSGQGPYNVV
                     WNTPGNYNVTVDVSSTGCSNVSASQGVTIQAVPNASISAAAAVCAGQNNAINFNGTAG
                     AGATYAWTFGGGTVTSGSGAGPYNVNWAAPGNPNLQVIVTENGCRDTANFAVTVNAIP
                     TSPFTVPSSACEGTPMTVTYTGTAGGAANYSWNFNGGTVLSGSGQGPYSIQWNNAGNY
                     NVSLTVTENGCTSAPTSVAVTMNDYPVAAFTADNAVCIGEDNTINFSGTAIAGATYNW
                     NFGSGTVGSGSGSGPYVVHWANAGNETVTLIVNQNGCRDTSTFNVRVNPIPNSTFSLP
                     TPVCEGTPMSFAYTGTAGAGATYNWTFTNGTVQSGSGPGPLNVLWNDAGTYAVSLTVT
                     ENGCTSPVTNQQVTLYDYPVAAVSGSPTVCVNGDNAISFTGTAIPGATYSWNFGNGTL
                     VSGSGAGPYTVRWAVDGTATVSVLVSQNGCSDSSTFNVRIYPIPTSAFSIPPNVCVND
                     PLSISYTGSASATATYTWNFGSGSVLAGSGSGPYSVVWNTSGNPAVTLTVTENGCVSP
                     VTTLNTFIAPLPVPHAGLDVGDCSGTSVNIGAPPIAGETYSWSPALGLADPAASSTSV
                     SLNNIGTNNRTDRYILTVTSSYGCVNRDTVDVTAFPVPAATFARPTGQCLEDNAFTFN
                     AGGLVFPGVDLQWDFSPSASTPTSTDQNPQPVSYSAPGVYTVTLHSSYNGCPGPDYID
                     SIEVFGMPVASFEPLVINGCEPLTVPFLNTSVGDINTYEWNYFDGAGDAIAEPSHTFE
                     KAGTYTVMLVVTSNEGCRADTILPNIITVWPQPVAGFIPNPSVTTIWEPVISFDNTTI
                     KADSYKWDFGDSTSTSLHEPTHRYTQVGAYLVTLYTINQYGCADTAIGTIRVEQGFNF
                     YVPNAFTPNGDGVNDTFRGLGTSFKTYELWIYNRWGRQIYYTNDYEKPWDGSMDDPVQ
                     NEVFTYRIRVVDIFDKDHTYLGHVSVVR"
     gene            3626..4219
                     /locus_tag="P5292_01115"
     CDS             3626..4219
                     /locus_tag="P5292_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRS37753.1"
                     /translation="MHQNTPLYGLVLAGGESRRMGSAKAALEYHGLPESDRMVDLLRR
                     SCTSVYVGCREDQLSSLPEHHLPISDLPRFAGNGPIGSLLSAWTVFPDVDWFVVGCDY
                     PFFGVPAVEYLLQADASEGWSGFHNNETQEPEPLLGIYRQSLQETLFQYFEKGGRSLR
                     KFLHSLEQDPHLPEDPRWIRSIDTREDYLLTLQTLRS"
     gene            4216..5070
                     /gene="fdhD"
                     /locus_tag="P5292_01120"
     CDS             4216..5070
                     /gene="fdhD"
                     /locus_tag="P5292_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRS37754.1"
                     /translation="MNQSSVSNVRIRRFSGGSWSEDPDLVVREEPLEIRLGFGPRESR
                     EQESISVTMRTPGHDFELALGFLRTEGVIQHPQDVHTVRYCTDGGRKEEQENIVRVEL
                     NEAVPVDLARLQRHVYTSSSCGVCGKTSLEAVHQLCPSTQSAHAQNFQLDAIKLTTVP
                     EAVRNAQTVFTHTGGLHAAALVDSSGTVRLLREDVGRHNAVDKLIGASFAKGDELLDT
                     YALFLSGRAGFELVQKAAMAGIPLVASVGAPSDLAVSLAQTTGITLVGFLREGRFNVY
                     SHPQRIRH"
     gene            5165..>7042
                     /locus_tag="P5292_01125"
     CDS             5165..>7042
                     /locus_tag="P5292_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008504090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HRS37755.1"
                     /translation="MAEEKDSALPNPENPERFTGLRLGKLKTAAAGIPGVAAAAVHVF
                     REMDVARGMKALLKLNQKDGYDCPGCAWPDPDDERSTIAEYCENGAKAIAEEATTRKL
                     GPEFFAANSVAALSQLNDYEIGKKGRIAQPMFLGKGETHYRPINWDDAFRKIAAHLNK
                     LASPDEAIFYTSGRTSNEAAFLYQLFVREYGTNNLPDCSNMCHESSGVALNESLGIGK
                     GSVTLEDFNHAEVIIILGQNPGTNHPRMLTALKKAKENGAVIISVNPLPETGLMGFND
                     PQTAKGILGIKARLTDIFLQVRINGDLALLQLINRALIEAERSEPGKVLDHDFIRDHT
                     SGFDAYTQEIFRSSKQELLRNCGISQKKIDETVEVLKYRKRIIACWAMGLTQHKNAVD
                     TIKEVVNLLLLKGSIGKPGAGTCPVRGHSNVQGDRTMGIYEKPSPKFLEQIERSFGFK
                     PPQEHGYDTVECIRAMHEGKAKVFIGMGGNFLSATPDTVYTAEALRKCALTVHVSTKL
                     NRSHLIHGEEAIILPTYGRSDLDVINGERQFISCENSMGVVQLSKGSLRPISDQLLSE
                     PVIVSRLAKATLGSRSRIDWNSFEQHYDRIRDAIERTIPGFGRYNERVREPGGFYLPN
                     CT"
BASE COUNT         1624 a         2008 c         1871 g         1539 t
ORIGIN      
        1 agaacgacac cctcatcatc gatgcggttt acctttcacc tgaattcggt cagatcaccg
       61 tacccaacgt gaacgcgaac ggtatgcccg gtgtcagtac actgagcagt accagtggta
      121 acatcgcgct tcaacaggtt cagatcatcg gacaaccagg caacgtaggt tatcacacga
      181 tctttctcga aggaacggat aatggtacgc ccgctcaaac agccagtgca ccgatcgtga
      241 tccgcgtcat tccggcgccg aattccaact tcaccgttag ccctacaggc attcagatgc
      301 ccggcaccct gttcaccttc accaacacca gcgtgggcgg cttcagcgcc gtatgggatt
      361 ttggcgacgg gtcgcctcct tcctcacttt acaatacctc gcacatctac ggcagcggcg
      421 gtacgtacac cgtcaccctg acggtcacca gccccaacgg ttgcaccgac gtctttacgc
      481 aacaagtgca gatccagcaa tgcggaaccg cttcgtttac tacctccaac gtgtgcgcag
      541 gcagcccctc actcatcacg ttcacgggta cagcgggcgt aggcgccacc tacaactgga
      601 actttgccgg tggtaccgta gtgtcgggtt cgggccaggg tccctataac gtggtctgga
      661 acacaccggg taactacaac gtcacggtcg atgtctcttc caccggttgc tccaacgttt
      721 ccgcgtcgca gggagtaacg atacaagcgg ttcccaatgc ttcgatttcg gcagccgccg
      781 ctgtttgtgc gggacagaac aatgccatca acttcaacgg tacggccggc gcgggtgcca
      841 cctatgcctg gacgttcggc ggaggcacgg tcacttcggg ctccggcgcg gggccctaca
      901 atgtgaattg ggccgctccg gggaatccga atcttcaggt gatcgtaaca gaaaacggat
      961 gtcgcgacac cgcgaacttt gccgttacgg taaacgcaat tccgacctct ccctttacgg
     1021 ttccatcgtc agcatgcgaa ggcacgccga tgaccgtcac ctataccgga acggctggtg
     1081 gcgctgccaa ctacagttgg aatttcaacg gcggaacggt gctcagtggt tcgggtcaag
     1141 gtccgtacag catccagtgg aacaacgccg gcaactacaa cgtcagtctt actgtcaccg
     1201 aaaacggctg tacttccgca cctaccagtg tagcggtaac gatgaacgat tatcctgttg
     1261 ccgccttcac ggcggataac gcagtatgca tcggcgaaga caacaccatc aacttctccg
     1321 gaacggcgat cgccggggct acctacaact ggaacttcgg cagtggaaca gtaggttccg
     1381 gctcgggttc gggtccgtat gttgtccatt gggcgaacgc ggggaatgaa acggttaccc
     1441 tgattgtgaa ccagaatggc tgccgcgata ccagtacgtt caacgtgcgg gtgaacccga
     1501 ttccgaattc gactttcagc ttgcccactc ctgtttgcga agggactccc atgtcgtttg
     1561 cctacacggg cacagccgga gcgggcgcga cctacaactg gaccttcacc aacggtaccg
     1621 tgcagtccgg tagcggtccg gggccgttga atgtgttgtg gaacgatgcg ggcacctacg
     1681 ccgtgtcgct taccgtcaca gagaacggtt gcacctcacc ggtcaccaac caacaggtga
     1741 ccctgtatga ttatccggtt gccgccgtga gcggatctcc gaccgtatgt gtcaacggcg
     1801 acaatgcaat ctccttcacc ggtactgcga tccccggagc aacctatagc tggaacttcg
     1861 gaaacggaac ccttgtgtcc ggtagcggag ccggccctta cacggtacgc tgggcggtcg
     1921 atggcacggc aacggtttcc gtgttggtct cgcaaaacgg ctgcagcgat tcgtccacct
     1981 tcaacgttcg catctatcca atcccgacct cggcattcag catcccgccg aatgtatgcg
     2041 tgaacgatcc gctgtcaatt tcctacacag gaagcgccag cgctaccgcc acctatacct
     2101 ggaacttcgg cagcggttcg gtactcgccg gaagtggttc agggccctat agtgtcgttt
     2161 ggaatacttc cggaaatccg gctgttacgc ttacggtgac ggagaatggt tgcgtatccc
     2221 ctgttaccac gctcaacacg ttcatcgctc ccctgcctgt gccgcatgcc ggccttgacg
     2281 taggcgattg ctccggaaca tccgtcaaca tcggcgcccc tccgatcgcc ggagaaacct
     2341 attcctggtc cccggcattg ggtctggccg atcccgccgc gagctccacc tccgtttctt
     2401 tgaataacat tggaacgaat aaccgcacgg accggtacat tctcacggtg acctccagtt
     2461 atggatgtgt caaccgtgat accgttgatg taacagcctt cccggttcct gccgccacct
     2521 ttgcgagacc gaccggtcag tgtctggaag acaatgcctt cacctttaat gccggcggtt
     2581 tggtgttccc gggcgttgat ctccagtggg attttagtcc atccgcttcc acgccgacca
     2641 gcaccgatca gaaccctcaa cccgtgagct attcggctcc cggcgtttat accgtaaccc
     2701 tccattcttc ctacaacggt tgccccggac ccgactatat tgattcgatc gaagtcttcg
     2761 gcatgccggt cgcctccttc gagccgctgg tgatcaatgg ctgtgaaccg ctgaccgtac
     2821 ccttccttaa taccagtgta ggcgacatca acacctacga atggaactac ttcgacggag
     2881 ccggtgatgc gatcgccgaa ccttcccaca cattcgagaa agcaggcacc tataccgtca
     2941 tgttggtagt gacaagcaat gaaggttgcc gcgccgacac catccttccg aacatcatca
     3001 cggtatggcc gcagcctgtc gccgggttca tcccgaatcc atcggtaacg accatctggg
     3061 aaccggtgat cagcttcgac aataccacca tcaaagcgga ttcgtacaag tgggactttg
     3121 gcgacagtac cagcacctcc ctgcatgagc ccacgcaccg gtacacgcag gtgggcgcct
     3181 atctggtcac cttgtatacg atcaaccagt acggttgcgc tgacaccgcc attggtacaa
     3241 tccgcgtcga acaaggcttc aacttctatg tacccaacgc cttcaccccc aacggtgatg
     3301 gggtgaacga tactttccga gggttgggaa cctcgttcaa aacgtatgag ttatggatct
     3361 ataaccgttg gggtcgtcag atctactata ccaacgacta cgaaaaaccc tgggacggat
     3421 cgatggatga tccggtacag aatgaggtat tcacctaccg catccgggtg gtggacatct
     3481 tcgacaaaga ccatacgtat ctcggacacg tttcggtagt acggtaattg cacaggcaaa
     3541 acgctccttg tgatggacgc atgttcatga caaggagcgt tttcatttct ttacctttaa
     3601 gccatcggaa ttgccggcgc aaatcatgca ccagaacaca ccgctatacg gactcgtgct
     3661 tgccggtggc gaaagccgta ggatgggtag cgcaaaagct gcgctggagt atcacgggct
     3721 tccggaatca gatcgcatgg tggacttgct gagacgatct tgcacgagtg tctacgtcgg
     3781 atgccgtgaa gaccaactga gcagcttgcc cgaacatcat cttcccatca gcgacctgcc
     3841 gcgcttcgcc ggcaatggac ccatcggcag cttgttgtcg gcctggacgg tttttccaga
     3901 cgtggactgg ttcgttgtcg gttgtgatta tccatttttc ggcgtaccgg cagtggaata
     3961 tctcctgcaa gccgacgcct cggaaggttg gtcaggcttt cacaacaacg aaacccaaga
     4021 gcccgaaccg ttgttgggta tttatcgcca atccctgcag gaaacgcttt ttcaatattt
     4081 cgaaaaaggt ggtcgttcac tccggaaatt tcttcactcc ctggagcaag atcctcattt
     4141 accggaagat ccccgttgga ttcgctccat tgatacacgg gaagattatc tcctgacatt
     4201 acaaacccta cgatcatgaa tcagtcttcg gtaagcaatg tccggatcag gcgattcagt
     4261 ggtggaagct ggtcggaaga ccccgatcta gttgttcggg aagaaccgct ggagatacgg
     4321 ctgggatttg gtccgcggga atcacgggag caagagagca tttccgtcac catgcggacg
     4381 cccggacatg atttcgagct ggcactcggc tttctgcgga cagaaggtgt catacaacat
     4441 ccgcaagacg tgcacacggt tcgttactgc acggatggcg gtcgtaagga agagcaggag
     4501 aacattgtcc gggtggaatt gaacgaagca gtaccggtag accttgcgcg cctccaacga
     4561 cacgtttata cctcctcgag ttgcggcgtg tgcggaaaaa cttccctgga agcggttcat
     4621 cagctttgtc cttctacaca aagtgcacat gctcagaatt tccaattgga cgcaatcaaa
     4681 ctcacgacgg tgcccgaagc ggtacggaac gctcaaaccg tcttcacgca tactggcggc
     4741 ttacacgctg cagcgctcgt cgactcatca ggaaccgttc gtttgctgcg ggaagatgtc
     4801 ggccgacaca acgcagtcga taaactcatt ggagccagct tcgcaaaagg tgatgaatta
     4861 ctggacacat atgcgctttt cctaagcgga cgcgcgggct ttgaactggt tcagaaagcc
     4921 gccatggccg gtattccgct ggttgcttcg gtaggagcgc cgagcgatct ggccgtctcc
     4981 cttgcacaaa caacgggtat caccctggtt ggttttttgc gggaagggcg attcaatgtt
     5041 tattcacatc ctcaacgaat acgccactga cgaatcgtat tttacacgtg caataaggac
     5101 agtattttta ttgcgggatc ggcacctttg gcaacgattt caactaatcc acctacccca
     5161 ttgcatggca gaagagaaag actccgctct ccccaatcct gaaaaccccg agcgttttac
     5221 ggggctccgg ttgggaaaac tgaagaccgc tgcggccgga atacccggtg tagcggccgc
     5281 tgctgttcat gtgtttcgtg aaatggatgt tgcgcgcgga atgaaggcgt tgctcaagct
     5341 gaaccagaag gacggatatg attgtcccgg ctgcgcctgg ccggatccgg acgacgagcg
     5401 gtcgaccatc gccgagtact gcgagaacgg agccaaggcc atcgcagaag aagcgacgac
     5461 ccgaaagctc ggccccgaat tctttgctgc aaacagtgta gctgcccttt cgcaactcaa
     5521 cgactacgaa atcggaaaga agggccggat cgcgcaaccg atgtttttgg gtaagggcga
     5581 aacgcattac cgtccgatca actgggacga tgcgttcagg aagatcgccg cgcatctcaa
     5641 caaattggcg agtcccgacg aagcgatttt ctacacttcg ggccggacaa gcaatgaagc
     5701 ggcttttctt tatcaactat tcgtacgcga atacggcacg aacaacctgc ccgactgctc
     5761 caacatgtgc catgaatcat ccggtgttgc actgaatgaa tcattgggga tcggaaaggg
     5821 ttctgtaacg ctggaagact ttaatcacgc ggaagtgatc atcattctcg gtcagaatcc
     5881 gggaacgaat catccacgaa tgctgacggc cctgaagaag gcgaaagaga acggcgctgt
     5941 catcatttcg gtgaatcccc tgccggagac cggcctgatg ggattcaacg atccgcaaac
     6001 cgcgaaaggc atcctgggta tcaaagcccg gctcacggac atctttctgc aagtgcgcat
     6061 caatggcgac ctggcgttac tgcaactcat caaccgtgcg ctgatcgaag cggaacgttc
     6121 tgagccgggt aaagtgctgg accatgattt tatccgtgat cataccagcg gattcgatgc
     6181 gtacactcag gaaattttcc gttcgtccaa acaagagttg ttgcggaatt gtggcatatc
     6241 tcaaaagaag atcgatgaaa cagtcgaggt actgaaatac cggaaacgga tcatcgcctg
     6301 ctgggcgatg ggacttaccc aacacaagaa tgcagtcgat acgatcaaag aggtggtgaa
     6361 tctgctcttg ctgaaaggaa gtatcggaaa acccggtgcc ggcacatgtc cggttcgggg
     6421 ccacagcaat gttcagggtg atcgtacgat gggaatctac gaaaagccgt ctccgaaatt
     6481 ccttgagcag atcgaacgct ccttcggctt caagcctccg caagaacacg gttatgatac
     6541 cgtggagtgc attcgcgcga tgcacgaagg gaaggccaag gtattcatcg gcatgggcgg
     6601 aaattttctt tccgctactc cggataccgt ttataccgcc gaggcacttc gtaagtgcgc
     6661 gttgaccgtt cacgtctcca caaaattgaa ccgcagtcat ctcattcacg gcgaggaggc
     6721 gatcatactc ccgacttacg gccgaagcga tctggatgtg atcaacggtg aacgacaatt
     6781 catcagttgt gaaaattcaa tgggcgtcgt gcaactcagc aaaggcagcc tgcggcccat
     6841 ctccgaccaa ttgttgagcg agccggtgat tgtttcccgt cttgcaaagg cgacattggg
     6901 aagtcgcagt cggatcgact ggaattcgtt tgaacagcat tacgaccgta tccgtgatgc
     6961 catcgagcgt accattccgg gttttggacg atacaacgag cgcgttcggg aaccgggcgg
     7021 cttctacctg cccaactgta cg
//
LOCUS       DAOTON010000030         7040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_9303_length_7040_cov_3.399141, whole genome shotgun sequence.
ACCESSION   DAOTON010000030 DAOTON010000000
VERSION     DAOTON010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9303_length_7040_cov_3.399141"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1812
                     /locus_tag="P5292_01130"
     CDS             <1..1812
                     /locus_tag="P5292_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS37756.1"
                     /translation="ENARILANAIEWGGINALASWIHLSQTSDTVVAGNTSNVIVTFQ
                     TTGLPAGTYYSNIAVASNDPATPVVLVPCTLSITGDPIVALSDTCVAFGNIMQYTTQQ
                     QSFDVINNGCDTLFINSITSNAAEFTLGTYPSFILPGGYATVNVNFTSNTVGTFSGTI
                     TVANNDVDTTVCLTAETFAAPDINPDPISVSQTLDACGATGSSTFDIQNLGGSDLTFN
                     ITGIPSWANVTPASGTVTAGTVQTITVDFNTGTLAGGTQSVNLLVTSNDPRTPVVQVA
                     CSLNVNNNPCISYTFASNTCTGCVDFTATSINTPDTWSWDFGDGNTSVLQSPTHCYTA
                     NGTYTVTLIAANAAGSDTLVQTVNAIITGPVATTCYPVTQAYCCGIGITNVSIANINN
                     TSADAIDGYQDYTCTDTTTLLTNNQYWMTVSTGFTYVESVKAWIDWNNDAVLDPVNEL
                     VFSDSAQMTNHGGFVSVPGTAVLGQPLRFRVASDYSGNPTPTPCLDLQYGQIEDYSVF
                     LVFDNGVKAPGANIPFSVFPNPFNRSTNIDYALSGNAVVSVEVFNMIGEKVSSFVLNE
                     EQPAGRHTYTFDNASSGVYYVRLTVDGQAMTQKIVKM"
     gene            complement(1890..2399)
                     /locus_tag="P5292_01135"
     CDS             complement(1890..2399)
                     /locus_tag="P5292_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HRS37757.1"
                     /translation="MEPPVDIVLRSLAVYLFIVIAIRLFGKKELAQLSVIDLVFILLI
                     SNSVQNAMVGPDSSLAGGLIAATVLFGANFLLKNLLYRNPDINRLVSGDPVMLVHQGN
                     VIEENLRREKITRDELEAAAREHGVASIKDVDLAILERDGNISILSSNYTHRSRRRRV
                     HKSMLKQDN"
     gene            2517..2849
                     /locus_tag="P5292_01140"
     CDS             2517..2849
                     /locus_tag="P5292_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRS37758.1"
                     /translation="MRFEKHVFICTNERAPGGRPSCGEACGMELVKLFKKAIKDRGLK
                     GRMRAQRAGCLDACEHGPSVVVYPEGVFYGGVKPEHVDEIVEQHLIQDRPVERLIIDF
                     SKDTPSDD"
     gene            2842..3207
                     /locus_tag="P5292_01145"
     CDS             2842..3207
                     /locus_tag="P5292_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37759.1"
                     /translation="MTELPERYRRDLALVRETAEQLIRDFNWYNIPIRFSGDPFHAYE
                     ELREQIIPHIDRMQREEPTLFQSLLYRIDLDERKFRRQLQAPDSGTHLADLILQREFQ
                     KVLTRRYFSDKDESADSFD"
     gene            3371..5791
                     /locus_tag="P5292_01150"
     CDS             3371..5791
                     /locus_tag="P5292_01150"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HRS37760.1"
                     /translation="MKKVFRSLVLSLLLATAGSVANAQTAELQVIHNCADPVADSVDV
                     YVDGLIAIDNFAFRNATGFLTLPAGVDIHVGIAPKTSTSVSDTLVNYTINLASGQRYV
                     AIASGVLNPGSFAANPDGRATGFNLLVRDNIRDAAASSGEVDFIVLHGATDAPTVDVI
                     PQSSATALVAGAAYTDITSYLTVPAAAYRLNITPAGDNSTIVASYNVDLSTLGGGAAV
                     VFASGFLNPAANQNGPAFGLFAALPNGTVVEFSLNTNARVQVIHNAADPAAAVVDVYG
                     NSDPLIPDFAFRTATPFIDVPAGVDITIGIAPGTSTSVNDTIPGLSTTVNLTAGQTYV
                     VIANGVTAPGSFATNPDGRPINFGLFLKTTVRESATDGSNVDFFVLHGATDAPTVDVI
                     PQSSAAPLVDNAAYGDITGYLSVPASQYFLNITPGSDNSTIVASYNVDLSSLGGGSAV
                     VFASGFLDPAANQNGEAFGIYAALANGTVVAFPLNTNARVQVIHNSADPAAAVVDVYG
                     NSNPLIPDFAFRTATPFIDVPAGVDITIGIAPGTSTSVNDTIPGLSTTVNLTAGQSYV
                     VIANGVTAPASFAVNPDGRPTSFALYVKPAVREAGTDGGNVDFFAFHGATDAPTVDVI
                     ARGVATLVDDAAYGDFTGYLSVPAALYTLDVTPAAGSPVVASFAADLSTLGGGSAVVF
                     ASGFLDPTTNQNGEAFGLFAALANGTVVPFTNTTGIDESNIRFATGIFPNPANNRIQV
                     GLQVKNAANAQVQVTDVTGRIVLNRQAYLVDNQLLSLDIDQLPGGVYNLTVIEGSSLQ
                     SAPFVIAR"
     gene            5982..6857
                     /locus_tag="P5292_01155"
     CDS             5982..6857
                     /locus_tag="P5292_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRS37761.1"
                     /translation="MSQFSIKDIEALTGIKAHTLRIWEQRYSLPQPKRTESNIRYYDD
                     DDLKLLLNVAMLNRHGHKISEITRLSEQQIRDIALDISVNSTHQSTHIQGLIASMINL
                     DERAFEKILSTCVLQYGLQRTVMEVVFPFMSLVGAMWQTGSVNPAFEHFMSNLIRQKL
                     IVAIDAQEIRPVAHPKNFLLFLPEGEHHELGLLFANYIIRSRGHRSLYLGQNLPVSDL
                     ARVSERFVPDVVFTSVTTGLPREVVEGMVKELKDSFPGASILLTGRYFSEHATVNEDV
                     TIVQRPSDLDAFWSR"
     gene            complement(6881..>7040)
                     /locus_tag="P5292_01160"
     CDS             complement(6881..>7040)
                     /locus_tag="P5292_01160"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HRS37762.1"
                     /translation="EDVAVIIDAEHLCVKARGIRDEHRSTLTSSYHGKFLNESTRLEL
                     LKYIELGR"
BASE COUNT         1480 a         2129 c         1830 g         1601 t
ORIGIN      
        1 gagaacgccc gcatcctggc caacgccatc gagtggggcg gtatcaacgc actggcttcc
       61 tggatccacc tcagccagac ttctgacacg gtagtagccg gtaacacctc gaacgtgatc
      121 gtcacgttcc agaccaccgg tctcccggcc ggcacctact attcgaacat cgcagtcgcc
      181 agcaacgacc cggctactcc ggtcgtactg gtaccctgca cgctgtccat caccggtgac
      241 ccgatcgttg cactctccga cacctgcgtc gcattcggca acatcatgca gtacaccacg
      301 cagcagcaga gcttcgacgt gatcaacaac ggttgcgata ccctgttcat caacagcatc
      361 acctcgaatg ccgccgagtt caccctgggt acttacccgt ccttcattct tccgggcggc
      421 tacgctaccg tgaacgtaaa cttcacttcg aacacggtcg gtacattctc cggcaccatt
      481 acggttgcca acaacgacgt cgacacgacc gtttgtctga cggctgaaac cttcgctgct
      541 ccggatatca acccggatcc gatctcggtc agccagaccc tcgatgcctg cggagctacc
      601 ggctcatcga ccttcgacat ccagaacctg ggtggcagcg atcttacgtt caacatcacc
      661 ggaatccctt cctgggctaa cgtaacgcct gcatcgggaa ccgttaccgc cggaaccgtt
      721 cagacgatca cggtcgactt caacaccggc acgctggccg gcggaacaca gtcggtcaac
      781 ctgctcgtca cctcgaacga cccgcgcaca ccggtcgtcc aggttgcctg cagcctgaac
      841 gtgaacaaca acccgtgtat cagctacacc ttcgccagca atacctgtac gggttgcgtg
      901 gacttcaccg ctacttcgat caacacgccg gacacctggt cgtgggattt cggtgacggc
      961 aatacctcgg tgctccagag cccgacccat tgctacaccg ccaacggcac ctacaccgta
     1021 accctcatcg ccgccaacgc ggccggttcc gatacgctgg tccagacggt caacgccatc
     1081 atcaccggcc cggttgccac gacctgttat ccggtcaccc aggcatattg ctgcggcatc
     1141 ggtatcacca acgtttcgat cgccaacatc aacaatactt cggcggacgc tatcgacggt
     1201 taccaggatt acacctgcac tgacacgacc acgctcctga ccaacaacca atactggatg
     1261 accgttagca cgggcttcac gtatgttgaa agcgtcaagg catggatcga ctggaacaac
     1321 gacgcggtgt tggatccggt gaacgaactc gtgttctccg attcggcaca gatgaccaac
     1381 cacggtggct tcgtatccgt accgggtacg gctgtattgg gtcagccgct tcgtttccgt
     1441 gtggcttccg attactccgg caaccccacg ccgactccgt gtctggacct ccagtacggt
     1501 cagatcgaag actactccgt attcctcgtg ttcgacaacg gtgtgaaagc tcctggcgcg
     1561 aacattccgt tctcggtatt cccgaatccg ttcaaccgct ccaccaacat cgactacgcc
     1621 ctttccggca acgctgttgt ttcggttgaa gtcttcaaca tgatcggcga gaaagtcagc
     1681 tccttcgtac tcaacgaaga gcagccggcc ggtcgtcata cgtacacctt tgacaacgct
     1741 tccagcggtg tctactatgt acgcctgacg gtcgatggcc aggccatgac gcagaagatc
     1801 gtcaagatgt gatccgttcc cggtccatca gaaaagccgc tccgaaagga gcggcttttt
     1861 ttatttacct gtatggttgc tgctgccggt cagttgtcct gcttcaacat ggatttgtgg
     1921 acgcgtcgtc gccggcttcg atgcgtgtag ttcgaagaaa ggatgctgat gttcccgtcg
     1981 cgttccagga tcgctaagtc gacatccttg atgctggcga caccgtgttc gcgtgccgcg
     2041 gcttccagtt cgtcgcgcgt gatcttttcg cggcggaggt tttcctcaat cacattaccc
     2101 tggtgtacca gcatcaccgg gtcgccgctg accaggcgat tgatgtcagg gttccggtag
     2161 agcaggtttt tcaaaagaaa gtttgccccg aacagcactg tcgcggcgat cagcccgccc
     2221 gccagtgagc tatccggacc gaccatggca ttttgcacgg agttgctgat gagcaggata
     2281 aagaccaggt cgatgaccga caattgtgcc agttcttttt taccgaagag ccggatggcg
     2341 atcacgataa agagataaac ggccaggcta cgtagaacga tgtcaaccgg aggttccatg
     2401 cggtcagtat tttccggtca agttaatccg gatcggtacg catgcggaga cggacgctcc
     2461 ggagttttta cccggaggtt gtgggaacgc ttgcggagat gtttaccttt gagtcaatgc
     2521 gcttcgagaa acacgtattc atttgcacca acgaacgcgc tcccggcgga agaccttcct
     2581 gcggcgaggc ttgtggtatg gagttggtca agctcttcaa gaaagcaatc aaagatcggg
     2641 gactgaaagg acgcatgcgc gcgcaacgtg ccggttgcct cgatgcctgc gaacacggac
     2701 cctccgtagt cgtttatccc gagggagtat tttacggcgg tgtgaaaccg gagcatgtcg
     2761 acgagatcgt cgagcaacac ctgatccagg accgtcccgt cgagcgattg atcatcgatt
     2821 tttcgaagga cacaccttcc gatgactgaa ttgccggaac gctaccgtag agaccttgct
     2881 ttggttcgag agacagcgga gcaactgatt cgcgatttca actggtacaa catcccgatc
     2941 cgattttccg gcgatccctt tcacgcgtac gaggagttgc gggagcagat cattccccac
     3001 atcgaccgga tgcagcggga agagccgacc ctctttcaat cgctcctgta ccgtatcgat
     3061 cttgatgaac gaaagttcag gcggcaattg caagcaccgg attccggtac gcacctggcc
     3121 gacctgatcc tccaacggga atttcaaaag gtcctgaccc gccgttactt ttctgacaag
     3181 gacgagtcgg ctgactcatt cgattaatag tgtttttagt aaaaaagata gtaacactat
     3241 tattttgatt ttgttttgac aactgcatga aattttcgca ttaacacttt tttcaaaaca
     3301 tgccgggcag gcggttgttg ggttgcttgt tactgtaatc cgctctaacc aactaaaacc
     3361 tacctccagc atgaaaaaag ttttccgatc cttagtgctg tcgttactgc tagcgacggc
     3421 gggatcagtt gcaaacgcgc agacagccga actgcaagtg atccacaact gcgcggaccc
     3481 ggtcgctgat tccgtagacg tatacgtcga tggtttaatt gccatcgaca acttcgcctt
     3541 ccgtaacgcg acgggtttcc tgactcttcc ggcgggcgtc gatatccacg tgggtattgc
     3601 tcccaagacc agcacatccg tttccgatac gctggtgaat tacaccatca accttgcttc
     3661 gggtcaacgc tatgtcgcga ttgccagcgg ggtgctgaat cccggcagct ttgcggccaa
     3721 cccggacgga cgtgctaccg gcttcaacct gctggtgcgc gacaacatcc gtgatgccgc
     3781 agcgtcctcc ggcgaagtcg atttcatcgt cctccacgga gcaaccgatg ctccgacagt
     3841 cgatgtgatc ccgcagagca gcgcaacggc attggttgca ggcgcggctt acacggatat
     3901 cacctcgtac ctgacggttc ccgcggcggc atatcgtttg aacatcactc cggcgggtga
     3961 caattccacg attgtcgctt cgtacaatgt cgatctgagc accctgggcg gaggcgccgc
     4021 agtggtattc gcttccggtt ttctgaaccc ggctgcgaat cagaacggcc cagctttcgg
     4081 actctttgcc gcattaccga acggaacggt tgtcgagttt tcactcaaca ccaacgcacg
     4141 cgttcaggtc attcacaatg ccgctgatcc ggcagccgcg gtggtcgatg tctacggcaa
     4201 cagcgatccg ctgattcctg acttcgcatt ccgcacggct actccgttca tcgacgttcc
     4261 tgccggagtg gacatcacca tcggcatagc tcccggcacc tctacctccg tcaatgatac
     4321 catcccgggt ctcagcacca cggtgaacct gaccgccggt caaacctatg ttgttatcgc
     4381 caatggcgtg acagctcccg gctcgttcgc taccaatccc gacggccgtc cgatcaattt
     4441 cggtctgttc cttaagacca ctgttcggga gagcgcaact gatggatcga atgtcgactt
     4501 ctttgtgctc cacggcgcca ccgacgcgcc gacggtggac gtcatcccgc agagcagcgc
     4561 tgcgccgctg gtcgacaacg ccgcatacgg tgacatcacc ggttacctca gcgtaccggc
     4621 ttcccagtac ttcctcaaca tcaccccggg tagcgacaac tcgaccatcg tggcttccta
     4681 caacgtagac ctgagctccc tcggcggcgg ctctgcggtc gtgttcgcat ccggtttcct
     4741 tgatccagcc gccaaccaga acggcgaagc gttcggaatc tatgccgcgc tggccaacgg
     4801 tacggtcgtg gccttcccgc tgaacaccaa cgcccgcgtg caggtgatcc acaattccgc
     4861 cgatccggct gccgcagtag tcgacgtata cggcaacagt aatccgttga ttcccgattt
     4921 cgcgttccgc acagccactc cgtttatcga tgttccggcc ggtgttgaca tcacgatcgg
     4981 tatagctcca ggtacctcca cttcggtgaa cgatacgatc ccgggtctaa gcacgacggt
     5041 gaacctgacc gccggtcaga gctacgtggt gatcgctaac ggcgtgaccg ctcccgcttc
     5101 cttcgctgtc aaccctgacg gccgcccgac cagcttcgcg ctgtatgtca agcctgctgt
     5161 acgggaggct ggtaccgatg gcggtaacgt cgacttcttc gccttccatg gtgcaactga
     5221 cgctccgact gttgatgtga ttgcgcgcgg tgtcgccacc ttggtggatg atgcggcgta
     5281 tggtgatttc accggttacc tcagcgtacc cgcggcactt tacaccctcg atgtaacgcc
     5341 ggctgccggc tcaccggtgg tggccagctt cgccgctgac ctgagcacac tcggtggtgg
     5401 ttccgcggta gtgttcgctt ccggcttcct ggatccaacc accaatcaga atggcgaagc
     5461 cttcggtttg ttcgccgctc tcgctaacgg aaccgttgtt ccattcacca acaccacggg
     5521 catcgacgaa agcaacatcc gtttcgccac cggtattttc ccgaatcctg ccaacaaccg
     5581 tatccaggta ggtttacagg tgaagaacgc cgctaatgcc caggtacaag taacggacgt
     5641 gaccggccgc atcgtgttga atcgccaggc ttacctggtc gacaatcaat tgctcagcct
     5701 cgacatcgac caattaccgg gtggagtgta taacctcacg gtgatcgaag gctccagcct
     5761 ccagagcgct ccgttcgtga tcgcacgttg atatttttgg gttagtttag acaaggcagg
     5821 tccggcgaat ttatccgccg ggcctgtttt ttatttattt gcagccattt ttgacctatt
     5881 tgccgtttct cacaaagaaa cctgaacaac agggccggat agctgttatt ttcgaatccc
     5941 cgttttatcc gggaaatgcg gaaacaagac cttcagtttc tatgagccag ttctccatca
     6001 aggacattga agccctaacc ggcatcaagg cgcatacgtt gcgtatctgg gagcagcgtt
     6061 acagcctgcc gcaacccaag cgtacggaga gcaatatccg ttattacgac gacgacgatc
     6121 tgaaactctt gctgaatgtc gccatgctca accggcatgg gcataaaatc tcggagatca
     6181 cccggctgag cgaacagcag atccgtgata tcgcgctcga catatcggtc aactccaccc
     6241 accagtcgac gcatattcag ggcctgatcg cgtcgatgat caacctcgac gaacgggcgt
     6301 tcgaaaagat cctgtcgacc tgtgtactgc agtatggttt acagcgcacg gtcatggaag
     6361 tggtattccc gttcatgagt ctggtcggtg ccatgtggca gaccggatcg gtcaatccgg
     6421 ctttcgaaca tttcatgtcg aatctgatcc ggcaaaagct gatcgtggcg atcgacgcgc
     6481 aggagatccg gccggttgct catccgaaaa atttcctgct cttcctgccg gaaggagagc
     6541 atcacgaact cggattgttg ttcgccaact acatcatccg ttcgcgcggt caccgttcgt
     6601 tgtaccttgg acagaacctg ccggtttccg acctggcccg tgtaagcgag cgctttgtgc
     6661 ctgacgtggt gttcaccagt gtaacaacgg gtctgccacg cgaggtcgtg gaaggcatgg
     6721 tgaaagaact taaagacagt tttccgggcg cgtcgatcct gttgaccggt cggtattttt
     6781 ccgagcatgc aacggtgaac gaagatgtca ccatcgttca gcgaccctcc gatctcgacg
     6841 ctttctggtc gcgctaacga ttgttatttc gggccggcag tcagcggccc agttcgatgt
     6901 acttcaacag ttcgagtcgc gtactttcgt tcaggaattt tccatggtac gaactggtca
     6961 gcgtgctgcg gtgttcatcg cggataccgc gtgctttcac acagaggtgt tccgcatcga
     7021 tgatcacggc gacatcttcc
//
LOCUS       DAOTON010000031         6983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_9388_length_6983_cov_3.399249, whole genome shotgun sequence.
ACCESSION   DAOTON010000031 DAOTON010000000
VERSION     DAOTON010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9388_length_6983_cov_3.399249"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="P5292_01165"
     CDS             complement(<1..700)
                     /locus_tag="P5292_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRS37763.1"
                     /translation="METLIQQGPELKTEGDFLPIHGTDYIELYVGNAKQAAHFYKTAF
                     GFQSLAYAGLETDVRDRASYVLRQGKITLVLTTPMSSATPIADHIRQHGDGVKVIAFW
                     VDDATYAFNETVRRGAQPYMEPTTESDAFGNVTRSGIHTYGETLHIFVERKNYQGVFL
                     PGYEKWESEYNPTPIGLEYVDHCVGNVEMGQMNRWAKFYEDVLGFKNLITFDDKDIST
                     EYTALMSKVMSNGNG"
     gene            complement(726..1898)
                     /locus_tag="P5292_01170"
     CDS             complement(726..1898)
                     /locus_tag="P5292_01170"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRS37764.1"
                     /translation="MPIYHRQGLLPAKRHVVFRQPNGQLYQEELVGTQGFSGMSSLVY
                     HLHPPTRVKQTGKPFGILPKVAVENNMRSLSFHGLEIPAEPDYLRSRKILFMNDDMRI
                     GLMAPSETAGYFFKNADADEMLFVHKGSGTLKTMFGSIRFGYGDYLVIPRGTVYQLNF
                     DDAENRLLLVESNGPILTPRRYRNDFGQLLEHSPFCERDFRRPENLETHAEPGDFEIM
                     IQKRGTIFPYTYAFHPFDVAGWDGYHYPYAFSIFDFEPLTGRIHMPPPIHQTFEGRNF
                     VICSFVPRMYDYHPEAIPAPYHHSNIDSDEILYYVDGDFMSRNNIHKGQLTLHPAGIP
                     HGPHPGAIERSIGQKETKELAVMIDPFHPVMITETALELEVPEYYLSWLDKETTNA"
     gene            2138..3772
                     /locus_tag="P5292_01175"
     CDS             2138..3772
                     /locus_tag="P5292_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37765.1"
                     /translation="MLQFDADHATEKADILNTLLGRPIRSVKVFTAYHDALLLAEAYA
                     PDENLYARCRQELQRLADLSLQWWNSTDERKRYALSGTGIANSLLVGSFSFELVQWLV
                     KRFPESFGLEGQDETELPFQDLLYQGLHPSENGVTEERTLELKPWLARFNGDQRLLSR
                     LVRIFQELPVSQELRDLLYENQKLYVEARLDKPELTRTLARFSAEPIYFHSSLLRKIN
                     FEEEISRPLPKPISLNERQRQSLWTNARVTLFNLFRETDPVTFTDPEETRWYPVGRGT
                     GIALFFLRPNRRLPLDSYVGYVAYKNGLPQAYGGSWIFRGQAKIGINIFPAYRGGESS
                     WLFAQLIRTYSQAFGVKTFAVEPYQIGKNNPEGIESGAFWFYYRHGFRPLQAELRTLA
                     AEEFDKINAIRRYKSASSTLRRLAESDLLLLPDGSDAQPFPIRYSRWMTEQFSSHFDG
                     DRKKFVSKSIQFCKRLGLLPERILNVQERQSLELLAPFVMAVYGNTTAPPPSATVHLS
                     NWLLSRYQKSESEWQELSGKCVHLFERLDALLSGDR"
     gene            complement(3831..>6983)
                     /locus_tag="P5292_01180"
     CDS             complement(3831..>6983)
                     /locus_tag="P5292_01180"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRS37766.1"
                     /translation="RESIIRNPQGEPIFEMRGVEVPAGWSQVATDILAQKYFRRTGVP
                     RTDGTTGGETSVRQVVHRLTSCWVEWGKRYGYFQEERDAHIFYEELVYMLLAQMAAPN
                     SPQWFNTGLYSVYGITGQPQGHYYVDPDTGELRQSASAYERPQPHACFILSVKDDLVN
                     PGGIMDLWVREARIFKYGSGVGTNYSSIRGEHERLSGGGYSSGLMSFLKIGDRAAGAI
                     KSGGTTRRAAKMVCLDIDHPEIESFILWKAKEEQKVAALIAAGYPSDYEGEAYATVSG
                     QNSNNSVRVPDTFFSALAQNRNWDLRARTDGSVIRSVSAQKLWEDIGDAAWHCADPGL
                     QFDTTINDWHTCPNGGRIRASNPCSEYMFLDDTACNLASLNLIRFYDEVTGRFNVEAY
                     EHACRLWTVVLEISVLMAQYPSEEVAQNSYDYRTLGLGYANLGSLLMVAGMAYDSPEA
                     RAFAASLTAIMTGTAYATSAEMASALGPFAKFKENRDAMLRVIRNHRYAAYHADDAYE
                     GLSVKPEGIDAGRCPDYLLKAACHAWDNALQEGERHGFRNAQVSVIAPTGTIGLVMDC
                     DTTGVEPDFALVKFKKLAGGGYFKIVNQSVSKALKRLGYSPGQIQDIEQYAKGHGTLY
                     GAPVINHETLEAKGLSKAEIRAIDSLCLSAFDIRYAVTVWSLQDESLQKLGITTAQKK
                     DPDFSLLEHLGFTEEQIEEANAFVCGTMTVEGAPHLREEHLPVFDCANRCGKIGQRFI
                     EPLGHIRMMAAVQPFISGAISKTINLPHESTRKDILEAYHFGWEKGLKAVAIYRDGSK
                     LSQPLSARTKKKDAPAAPAALTQEINSELLTADQVLDAARRIIQSSSDTVFKRQLSNI
                     VQRKRLPARRSGFTQKAKVGGQTIFVRTGEYDDGTLGEIFIDMHKEGASFRSLLNCFA
                     IAVSIGLQYGVPLEELTEKFIFTRFEPSGPVEHPNIRSATSVIDYIFRLLALEYLDRT
                     DLVQVPPQAPVSHKSSVPIEVRHTQPSEINTELAGQQTQDYLAGMMGDAPVCHNCGHI
                     TIRSGSCYKCLNCGTSLGCS"
BASE COUNT         1601 a         1895 c         1862 g         1625 t
ORIGIN      
        1 ggccgttacc attcgacatc accttactca tcaacgcggt gtactcggta ctgatatcct
       61 tatcgtcgaa ggtgatcagg ttcttgaaac ccagcacatc ttcgtaaaac ttcgcccagc
      121 ggttcatctg tcccatctcc acattgccta cacaatggtc gacgtactcc aagccgatcg
      181 gcgtaggatt gtattcactt tcccattttt cgtaacccgg caggaagacg ccttggtagt
      241 tcttgcgttc cacgaaaata tgcagggtct ctccgtaagt atgaataccg gatcgggtca
      301 cattaccgaa cgcatccgac tcggtggtgg gttccatata gggttgcgca ccacggcgaa
      361 ccgtttcgtt gaaggcatag gtcgcgtcat cgacccagaa ggcgatgacc ttcacaccat
      421 cgccgtgctg ccgaatgtga tcagcgatcg gtgtggctga actcatggga gtcgtcagca
      481 ccagggtgat cttaccttgg cgcaagacgt aggacgcccg atcccggaca tccgtttcga
      541 gccccgcgta cgccagcgat tggaatccga aagcggtttt ataaaagtgg gcggcctgtt
      601 tggcatttcc cacatacaat tcgatataat ccgttccgtg aatcggcaga aaatcgcctt
      661 cggttttcag ttcaggacct tgttggatca gcgtttccat ggtgatggta tggttttgtc
      721 aataatcagg cgttggtagt ttctttgtcg agccaggaga ggtaatactc cggtacctcg
      781 agctcgaggg cggtttcggt gatcatgacg ggatggaacg ggtcgatcat caccgcgagt
      841 tcttttgttt ccttctgacc gatgcttcgt tcgatcgccc cgggatgcgg gccgtgcgga
      901 ataccggccg gatggagcgt caactgacct ttgtggatgt tgtttcggct catgaagtcg
      961 ccatcgacat aatacagtat ctcgtccgaa tcgatgttgc tgtggtgata aggtgccggg
     1021 atcgcttccg gatggtagtc gtacatgcgc ggcacgaagg agcagatcac aaaattccgg
     1081 ccttcaaacg tctggtgaat aggaggcggc atatggatgc ggccggtgag gggctcgaag
     1141 tcgaagatgg agaacgcgta aggataatga tagccatccc aacccgcgac atcgaacgga
     1201 tggaacgcgt aggtgtacgg gaaaatcgtc ccccgcttct gaatcatgat ctcgaagtcg
     1261 ccgggctcgg catgtgtttc caggttttcc ggacggcgga aatctcgttc gcaaaacggc
     1321 gagtgctcga gcaattggcc gaaatcattg cggtatcgac gtggtgtaag gatcggccca
     1381 ttggactcga cgagtaagag acgattttcg gcatcatcga aattcaattg atagaccgta
     1441 ccccggggaa tgaccaggta atcgccgtat ccgaaccgaa tcgaaccgaa catggtcttc
     1501 agggtaccag accccttgtg tacgaagagc atctcgtccg cgtcggcatt cttgaagaag
     1561 taaccggctg tttcggacgg cgccatcaac ccgatgcgca tgtcatcgtt catgaacagg
     1621 atcttccggc tgcgcaaata atccggctcc gcgggaatct ccagtccgtg aaaactcagg
     1681 cttcgcatgt tgttctcaac ggccactttc ggtaggatgc caaaaggctt tcccgtctgc
     1741 tttacccgtg tcgggggatg caggtggtag accagagagg acatgccact gaaaccctgt
     1801 gttccgacga gttcttcctg atagagttgg ccgttgggtt gacgaaatac gacgtgccgt
     1861 tttgcaggca gcaacccctg tcgatggtag atgggcataa cgaatcggtt ggttttcaaa
     1921 attgcttttt caactaagca taccgaatga ccatcgtcac atcagttcct gactaaaatc
     1981 atctcaccag ccacgtcaag ggtcggaact tggtatgcct tgccagatga tgcgcggagc
     2041 gaaaaacccc aaattgccag tacgctccag aaagcgggac gtgaagccta aaatcacgcc
     2101 aattggggag tctgaggtat tggcatttcg gaatacgttg ctccaattcg atgcggatca
     2161 cgccaccgaa aaagcggaca tcctaaacac cctgctcggt cgaccgattc gctccgtcaa
     2221 agtcttcacc gcttaccatg acgcattgct attagctgaa gcctatgctc cagacgaaaa
     2281 cctgtatgcc cggtgtcggc aggaactcca gcgcttagcc gatctttccc tccaatggtg
     2341 gaacagcacg gatgagcgaa agcgttatgc actatccgga accgggatcg cgaattcatt
     2401 gctggtcgga tccttcagct ttgaactcgt tcagtggttg gtgaaacggt ttcctgaatc
     2461 gttcggactg gaaggacaag atgaaaccga gcttcccttt caggatctgc tttatcaggg
     2521 ccttcacccg agcgagaacg gggtaaccga agaacgcacg cttgaactaa aaccctggtt
     2581 ggccaggttc aacggtgatc aacgacttct aagcagactg gtcaggatct ttcaggagtt
     2641 gccggtctca caagaattgc gcgacctctt gtatgaaaat cagaaattgt atgtagaagc
     2701 ccgtttggac aagccggagt tgaccagaac gttggcacgt ttttccgccg agccaattta
     2761 cttccacagt tcgctacttc gcaaaattaa cttcgaggaa gaaatatccc gaccactgcc
     2821 aaagcctatt tccctgaatg agaggcagcg acaatccctt tggacaaacg ctcgcgttac
     2881 cttgttcaac ctctttcggg agacagaccc ggtaaccttt acggatccgg aggagacacg
     2941 atggtatccg gtcggacgtg gaacggggat cgcgttgttc ttccttcggc cgaatcggcg
     3001 cttaccgctt gatagttatg tgggctacgt tgcctacaag aatggtttac cacaagccta
     3061 cggcggcagt tggatcttcc ggggacaggc caagatcggc atcaacatct tcccggcata
     3121 ccgcggaggt gagtcttcct ggctgttcgc ccaactgatc cgcacgtatt cacaagcgtt
     3181 cggggtgaag acattcgcgg tggaaccgta tcagattgga aagaacaatc cggaagggat
     3241 cgaaagcggc gccttctggt tctattatcg ccatggtttt cgaccgcttc aggcggaact
     3301 gcgaacgctg gctgcagagg aatttgacaa gatcaatgct attcgtcgat acaaatccgc
     3361 gtcctccacg ctccgcaggc tggcggaatc ggatctgttg ctgttacccg atggaagcga
     3421 cgcccaaccg ttcccgatcc gctatagccg gtggatgacg gaacagttct ccagtcactt
     3481 cgatggtgac cggaaaaaat tcgttagcaa atcgatccaa ttctgtaagc ggctcggatt
     3541 gctacccgaa cggattctca acgttcagga gcgacaaagt cttgaactgc tggcaccctt
     3601 cgtcatggca gtgtatggca acacgacggc gcctcctcca tcggccacgg tccacttaag
     3661 caactggctt ttgtcgcgct atcaaaaatc cgaatccgag tggcaggagc tttcgggaaa
     3721 atgcgtgcat ctcttcgagc gactggatgc actactctcc ggggatagat gaaatacgca
     3781 gacaataaaa caagcggatc aggttggatc cgcttgttag ttgcctatgc tcagctacaa
     3841 cccaggctcg ttccgcaatt caggcacttg taacaactgc ccgaccgaat ggtgatgtgt
     3901 ccacagttat gacagacagg agcgtcgccc atcatgccgg caaggtaatc ttgtgtttgc
     3961 tgacctgcca gctccgtgtt gatttccgaa ggttgggtgt gcctgacttc gatcggcacg
     4021 gacgacttgt gtgagaccgg agcctgggga ggcacctgga ccaggtccgt tcgatccaga
     4081 tactccaacg cgagtaaacg gaagatgtaa tcgattaccg aagtggcgga acgaatattc
     4141 gggtgttcca ccggtccgga cggttcaaaa cgggtgaaga tgaatttctc cgtcaactcc
     4201 tccaggggta caccgtactg cagaccgatg gacacggcga tggcgaagca gttcaacagc
     4261 gaccggaagg aggcaccttc cttgtgcatg tcgatgaaaa tttcccccaa agtaccatcg
     4321 tcatactcac cagttcgaac gaaaatcgtt tgtccaccca ccttcgcctt ttgtgtaaaa
     4381 ccgctgcggc gtgcaggaag tcgtttgcgt tgcacaatgt tcgaaagctg ccgtttgaaa
     4441 accgtatcgg agctcgactg gatgatgcgt cgtgccgcat ccagcacctg atcagcggtc
     4501 aggagttcgc tgttgatttc ctgcgtcagt gcagcaggtg cagcaggtgc atccttcttt
     4561 ttggttctgg cggacaaggg ctgcgacaat ttggatccgt cgcgataaat ggcaaccgcc
     4621 ttcaggccct tctcccagcc gaaatggtaa gcttccagga tatccttccg ggttgattca
     4681 tgtgggaggt tgatcgtctt agaaatagcg ccggaaatga acggctgtac cgcggccatc
     4741 atccggatgt gcccgagcgg ttcgatgaac cgctgcccga tcttcccaca ccgattcgca
     4801 cagtcgaaaa ccgggagatg ttcctcccgc aagtgaggtg cgccctcgac ggtcatcgta
     4861 ccgcaaacaa aagcgttcgc ttcctcgatt tgttcttccg taaagcccag gtgctcgagc
     4921 aggctgaagt ccggatcttt cttttgggcc gtcgtgatac cgagtttttg caatgattcg
     4981 tcctgcaggg accagaccgt tacggcatag cgaatatcaa aggccgacag gcacaaactg
     5041 tcaatggccc tgatctcggc tttggaaagg cccttcgcct ccagggtttc gtggttgatg
     5101 accggtgcgc cgtagagtgt accgtgtccc ttagcgtact gttcaatatc ctgaatctgc
     5161 ccgggcgaat agcccaatcg cttcagggcc ttggatacgc tttgattcac gatcttgaaa
     5221 tatccgccgc cggcgagctt tttaaacttc accaaagcaa aatccggttc aacgcccgtc
     5281 gtatcgcaat ccatgaccaa cccgatcgtc cccgtaggag cgatgacact cacctgggca
     5341 tttctgaacc catgacgctc cccttcctgc aaggcgttgt cccaggcatg gcaagccgct
     5401 ttgaggaggt aatcagggca tctgcctgca tcgatccctt ccggtttgac cgacaatcct
     5461 tcatatgcat cgtccgcatg ataggcggca taacgatggt tgcgaattac acgcagcatc
     5521 gcatcccggt tctccttgaa ttttgcgaac ggcccgagcg cggaagccat ttccgccgag
     5581 gttgcatagg cggtaccggt catgatcgcg gtgagcgaag cggcgaaggc tcttgcttcc
     5641 gggctgtcat acgccattcc tgctaccatg agcagggatc ccaggttggc atatccaagg
     5701 cccagggtcc gatagtcgta actgttttgc gcaacttctt cggacggata ttgggccatc
     5761 aagacggata tttcgaggac aaccgtccaa agccggcaag catgctcata agcctctaca
     5821 ttgaaacgtc ctgtcacttc atcatagaat cggatcaggt tcagtgatgc gagattgcat
     5881 gcggtatcgt cgaggaacat gtattccgaa cagggattgg acgcgcgaat gcgtcctcca
     5941 ttcgggcagg tatgccagtc gttgatcgta gtatcgaact gtaatccggg gtcagcgcaa
     6001 tgccatgctg catcaccgat gtcttcccaa agtttttggg cgctgacgga ccggatgaca
     6061 ctaccgtcgg tcctggcccg aagatcccaa ttccggttct gcgccagcgc ggaaaagaac
     6121 gtatccggaa cacgaaccga gttgttgcta ttctgtccgg aaaccgtggc gtaggcttcg
     6181 ccttcgtagt cggacggata tcctgcagcg atcagagccg ctaccttttg ttcttccttc
     6241 gctttccaga gaataaagga ttcgatttcg gggtgatcga tatccaggca gaccatcttc
     6301 gcagcgcgac gggtggtccc gcctgatttg atcgcacccg cggcacggtc tccgatctta
     6361 aggaacgaca tcaacccgga agaataaccg ccaccggaaa gtcgttcgtg ttctccgcga
     6421 atggaagaat agttcgttcc gacaccggaa ccgtatttga agatacgtgc ctcacgcacc
     6481 caaaggtcca tgattccccc cgggttgacc agatcatcct tcacggacag tataaagcag
     6541 gcatgcggct gtgggcgttc atatgccgaa gccgattgac gcaattcccc cgtatccgga
     6601 tccacatagt aatggccctg cggctgaccg gtgataccat aaaccgaata caagcctgta
     6661 ttaaaccatt gcggagaatt gggtgcggcc atctgagcga gcagcatata caccaattcc
     6721 tcatagaata tgtgcgcgtc ccgctcctct tgaaaatatc cgtagcgttt tccccattcc
     6781 acccaacagg acgtcaatcg atgaacaacc tggcgaacag aggtttcacc gccggtcgtt
     6841 ccatcggttc gcggcacgcc ggtgcgacga aaatacttct gggccagaat gtcggttgcc
     6901 acctgcgacc aacctgccgg cacctcgaca ccgcgcatct cgaagatcgg ctctccttgc
     6961 ggattccgga tgatcgactc acg
//
LOCUS       DAOTON010000032         6950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_9456_length_6950_cov_3.674547, whole genome shotgun sequence.
ACCESSION   DAOTON010000032 DAOTON010000000
VERSION     DAOTON010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9456_length_6950_cov_3.674547"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2492
                     /locus_tag="P5292_01185"
     CDS             <1..2492
                     /locus_tag="P5292_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37767.1"
                     /translation="RITNNGSGQVVVGNGSGDTYNTDVILSNNTPSSIFYFAHAGTNT
                     FSGDVQLNNTSTGGVQWGASNGTSTFAAGKAVYTGEYRSGPLQLWRITQSGTTDKVIQ
                     ATPTIFQVTNSTLITAINVQCTASSTLSGSNLQGNVRLRATDITLSGANQLATQGDSI
                     VFEKTGNTGNAWTGGNTFGKSGGYMELKVSSNGQLVLANSTRDIFLSNVAIRNQGSGI
                     LYVAHNDNSGTVFSGDLELSNSGTGSIRFGQSSGTTILATGKQLIIRDSVFTSGDLYL
                     RRFTQLGSTAINIDVRSGTGAIYFQGSTVFNGTLSLAFPQVYLNGSTFNGSTRIEKTG
                     ATDNNSSGDNTYNGPVLLANSGTGAFNLAVSNPDDYNAAATFQQHGSGAMNPAYNNSN
                     TFAGDISTLGTTTAITFGAGSGTVRVNGATAQTFSNEAGKPANVRRLTMSSTGGGLVL
                     NGPLTVSASMNLSSGIITTTDNNLLTIANGFSGVSNTSNTSYVNGPVRKTGNQSFTFP
                     VGKNGYYRPVSISAPSVATDQFIAEYFQVDPASFSDVTRLEAALNRVSRCEYWNLRRN
                     AGSSNVNVTLSWNTTSCGVSIPSQLRVARWDSTGTMWRNQGNGGISGNPTSGTMVSTP
                     AVTQFGQFTLGSSTANNALPIELSAFSAEAAGESVLVRWETMSEINNDYFSVERSADG
                     ENFEVLKTLPGAGNTTERKNYQWLDEQPLEGISFYRLRQTDYDGKYEVFDPVSVKRGE
                     ASEEFRLMSTAPNPFSEQFKAVIYLPQSGDVVFDLSDVNGSSVFNNRLPATNGVSVLQ
                     AAQLSHLQAGFYLLRIFVNDELKQSVKLLKQ"
     gene            2635..2708
                     /locus_tag="P5292_01190"
     tRNA            2635..2708
                     /locus_tag="P5292_01190"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2667..2669,aa:Cys,seq:gca)
     gene            2942..3976
                     /locus_tag="P5292_01195"
     CDS             2942..3976
                     /locus_tag="P5292_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37768.1"
                     /translation="MKTLISILLVCIGICTKTYSQCYPATVATNGSSATRGMYVDCFD
                     CIIADIENGNPLGLENELFNYCDSRCIKYIALFRLDQGGSCLPGGVIGDPVHEATLSQ
                     LILDLKARIPGMTVGAVGSSKSWFYGHFTPNFPVNYFDEKAKCEAPIVFSNDALSRIM
                     NPAPDEMNELTFIASELSKFFGRTAMYNSTDYNGNHAGKIDVFSVEYEYWDQSASSNS
                     QYLNYLSIIQALKSISCKSQYHETIETELKLKNLQSTGCPSCPSAYDQAQAIDKYLDR
                     ILLVDYRHLLTDLFTGTSAGSTHCAGLYLLGDKHTGKQSHVWPLFSAEYPTSLGGSTW
                     QTCEPHGVSA"
     gene            4052..4525
                     /locus_tag="P5292_01200"
     CDS             4052..4525
                     /locus_tag="P5292_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37769.1"
                     /translation="MYNVENDYYAVPLASASNTFCTACGCTDWKENNIPGASNPFHNL
                     IDGFMWFTYTLMKDHSVARIRQLTDPASQENILNEIPELIFNSMTNELSCLGNPLNNQ
                     IYIDLYSVQGKLISEYKLHRNQKIYLGDLNTGVYILRSHFSDKSEEKIIKIAVAR"
     gene            4534..5955
                     /locus_tag="P5292_01205"
     CDS             4534..5955
                     /locus_tag="P5292_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37770.1"
                     /translation="MNKYILAYLIFASCWVSKAIASDLIVPYSHFALRYNSSVKIIQL
                     ADSNFLILSRHKDIGSTPAMAGLTWCHRNGDTIKTKLLTLNSGSAAPLDIISLYDGSL
                     FISGTTDDPLDNTLFTIHLDSTGSLISFKLHTSIDAVWLSNSIQLDSGHVLLAATLVN
                     VPESIIFYKVNPDGDSLSFHNYTFPGLLGVSCSRLLNDSTILFGGDMSNSNFVLLTNL
                     NGDSLSIDTFPFTTSGKVHISSIAITGSQYLVIAGTSTDTLDSNHQSVWCMKTDLSGL
                     ILWQNRFHSANPFWNSSAISEIGTNYFAINTFYNTTTTIGNHPPVLIIDSNGDSVSMS
                     NASGNYCEMASGNNNSIIGDMNGNAFLTTTIDSVCSNIYQNIAFEKFDFSSLVNSIDV
                     LNNNHHHLDVFPNPANNHLNVIDFDNEIGHISLYDISGRFLFSKIKEESIQLPDIHNG
                     VYVVVLYHKDRTPLKKIRLVVLH"
     gene            6113..6679
                     /locus_tag="P5292_01210"
     CDS             6113..6679
                     /locus_tag="P5292_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37771.1"
                     /translation="MYSRRKKDMDAFEQIIGQLLVEEKYWVRHSVKIDLTPEEKRYIN
                     KPSTPRPEIDIVAYNTTTDTIYLLEVKSYLDSPGVVSEHVAIEQDEQSGRYKLLTAMN
                     YRTTLAKRLHADWCKSGHIRKSTRISFGLIAGKVYRNRELELRRYFQKQGWLFWGPSE
                     IKSRILRLSQKGYENNTVTIAAKILTRV"
BASE COUNT         1940 a         1580 c         1583 g         1847 t
ORIGIN      
        1 tccggatcac caacaacggt tcgggacagg tcgttgtcgg caatgggtct ggtgacacgt
       61 acaataccga cgtgatcctg tcgaataata caccttcgtc catcttttat ttcgctcatg
      121 ccggtaccaa taccttctcc ggcgatgtgc aactgaataa cacctccacc ggcggagtgc
      181 aatggggcgc gtcgaacggc acttccacat ttgcagccgg gaaagcggtc tataccggtg
      241 aataccggag tggcccgctg caactgtggc gtatcacaca aagcgggacg accgacaaag
      301 tgatccaggc cacgccgacc attttccagg tgaccaattc cacgctcatc acggcgatca
      361 acgtgcaatg cacggcctcg tcgacgctca gtggttccaa cctgcagggc aacgtacgac
      421 tccgcgcgac cgacatcacg ctatccggcg ccaatcaact ggcgacgcag ggcgattcaa
      481 ttgtattcga gaaaaccggc aacaccggca atgcctggac gggtgggaac acctttggaa
      541 agagtggtgg ctacatggag ttgaaagtaa gcagcaacgg tcagttggtg ctggccaaca
      601 gcacccgcga catcttcctg agtaacgttg ccatccgcaa ccaggggagc ggcatactgt
      661 acgtggccca taacgataac agcggcaccg tcttcagtgg tgacctggaa ctcagcaatt
      721 ccggaaccgg ctccattcgt ttcgggcaat caagcggaac gaccatcctc gcaacaggca
      781 agcaactgat catccgcgat tcggtcttca catccggcga cctgtatttg cgacgcttca
      841 cacaattggg cagcaccgcc atcaacatcg atgtacgtag cggcaccggc gcgatctact
      901 tccagggcag caccgtattc aacggcacgc tctccctcgc gtttccgcag gtgtacctga
      961 acgggagtac cttcaacggc agtacgcgca tcgagaagac cggcgcgacc gacaacaaca
     1021 gcagcggtga caatacttat aatggtccgg tcctgctggc caacagcggt accggcgcgt
     1081 tcaacctggc ggtttcgaat cccgacgatt acaacgccgc ggccactttc cagcaacacg
     1141 gcagcggcgc gatgaatccc gcctacaaca acagcaacac cttcgcgggt gatatctcca
     1201 ccctcggcac caccacggcg attacgttcg gagcaggcag cggcacggtg cgggtcaacg
     1261 gcgctaccgc gcaaaccttc agcaacgaag ccggcaaacc tgccaacgta cgtcgtctga
     1321 cgatgtccag caccggcggc ggtctggtgc tcaacgggcc gctcaccgta tcggcatcga
     1381 tgaacttgtc gagcgggatc atcaccacga ccgacaacaa cctgctgacc atcgccaacg
     1441 gcttcagcgg cgtgagcaat acgtccaata ccagttacgt gaacggcccg gtacggaaga
     1501 ccggcaatca gtcgttcacc ttcccggtgg gtaagaatgg ctactaccgt ccggtatcga
     1561 tctcggcgcc atcggtggca acggatcagt tcatcgccga gtatttccag gttgatccgg
     1621 cgagtttcag tgatgtcacg cgactggaag cggcgctgaa ccgtgtgagt cgctgcgaat
     1681 actggaacct gcggcgcaac gccggcagtt cgaatgtgaa cgtcacgctg agctggaata
     1741 ccaccagttg cggcgtttcg attccctcgc agttgcgtgt ggcacgctgg gacagcaccg
     1801 gcacgatgtg gcgcaaccag ggtaacggcg gcatctccgg aaacccgacc tccggaacga
     1861 tggtgtcgac gccggctgta acgcagttcg gacaattcac gctcggttcc tccacggcca
     1921 acaacgcctt gccgatcgag ttgtcagcct tctcggcgga ggctgccgga gaaagcgtgc
     1981 tggtgcgttg ggaaacgatg agcgagatca acaacgatta tttcagtgtg gagcgcagcg
     2041 ccgacggcga aaacttcgag gtattgaaga cgctgcccgg cgcgggtaac accacggaac
     2101 gcaagaacta ccaatggctc gacgagcaac cgctcgaggg gatcagtttc taccgcttgc
     2161 ggcagaccga ttacgacggc aagtacgaag tcttcgatcc cgtttccgtg aaacgcggtg
     2221 aagcatcgga agagttccgt ctcatgagta cagcgccgaa tccgttcagc gagcagttca
     2281 aggcggtcat ctacctgcca cagtcgggcg atgtggtttt cgacctgagc gacgtgaacg
     2341 gcagcagtgt gttcaacaac cgactgcccg ccaccaacgg ggtgagtgta ctgcaggccg
     2401 cgcagctgag tcacctgcaa gccggctttt acctgctgcg gatcttcgtg aacgacgagc
     2461 tgaaacagag tgtcaaactg ctgaagcagt gattttgcgc tcgacgagcc aactggctga
     2521 ttagcaggag gaaaatcgac taccgaaccg tcctcccgaa ggcaacatca ggagccgtac
     2581 gaccgtaaaa ttggcctcac agccgggaaa acgctatatt tgcgctcccc aaagggtcct
     2641 gtggccgagt ggctaggctg agctctgcaa aagctcctac agcggttcga atccgctcgg
     2701 gacctccaat ttcgcttcat tagaagacat caagcctcat ttcgcaagaa tgaggctttt
     2761 ttgttttcat taggagacgt taggactcgt taggacgcgg gattgattcg ctcacgattc
     2821 gctgagaggg ttttgggtga ttcgctaaac actggcgtct acggaaaaat gagatcattt
     2881 gatatttgat ttggtatttg aaaatttatt tcgtacttcg tataaactaa aattcaatat
     2941 catgaaaact cttatttcaa ttttattggt ctgcataggt atttgcacaa aaacatattc
     3001 gcaatgttac cctgcgacgg tagcaacaaa cggaagttca gctacaagag gtatgtatgt
     3061 agattgcttt gactgcataa ttgcagatat tgagaatgga aatcctcttg gacttgaaaa
     3121 cgaattattt aattattgcg atagtcggtg cattaaatac attgcacttt tcagacttga
     3181 tcaaggaggt tcatgtcttc ccggtggagt aattggagat cctgttcatg aggctactct
     3241 ttcacaattg attcttgatc tgaaagcaag aatacccggg atgacagtag gggcggtagg
     3301 gtcttcgaaa agttggtttt atggccattt tacgccaaac tttccagtaa attattttga
     3361 tgaaaaagca aaatgcgaag cacctattgt tttttctaac gatgctttgt caaggattat
     3421 gaatcctgcg cctgacgaaa tgaatgaact aacttttatt gcctcagaat tatccaaatt
     3481 ctttggtagg acggctatgt ataattcaac agattacaat ggaaatcatg ctggtaaaat
     3541 tgatgttttt tctgttgaat atgaatactg ggatcaatcc gcttcgtcta attctcaata
     3601 tctcaattat ctttcaataa ttcaagctct aaaatccatt tcttgtaaat ctcagtatca
     3661 tgagactatt gagacagagc taaaattaaa aaacttgcag tcaacaggct gtcctagttg
     3721 tccgtcagcg tatgatcaag cccaagcaat cgataagtac cttgacagaa ttttattggt
     3781 cgactatcga catctcctaa ctgatttatt tactgggaca tctgcaggaa gtactcactg
     3841 tgccggatta tacctattag gtgataaaca tactggcaag caaagtcatg tatggccgct
     3901 attttccgct gaatatccta cttctcttgg aggatcaact tggcaaactt gcgaacccca
     3961 cggcgtatct gcttgatgta atgcaaatga tgtatgcaac gactatctag gttattatgt
     4021 tggcgatgtt ccgggcacag ggtataatcc aatgtataat gttgaaaatg attattacgc
     4081 ggtcccatta gcaagtgctt caaatacttt ttgcactgca tgcggttgta cggattggaa
     4141 agaaaataat attcctgggg cttctaaccc gtttcataat ttgattgatg gttttatgtg
     4201 gtttacatat acgctcatga aggatcactc tgttgcgcgt attcgacagc taacagatcc
     4261 tgcaagccaa gaaaatattt taaatgaaat tcctgaactt atatttaatt caatgacaaa
     4321 tgaattatca tgcctaggaa atccattaaa taatcagatt tatattgatt tatacagcgt
     4381 ccaaggcaaa ttgatttctg agtataaact acataggaat caaaaaatat accttggcga
     4441 tttaaatact ggtgtttata ttttaagatc acacttcagt gataaatccg aagaaaaaat
     4501 aatcaaaatt gcagttgcta gataaaaaga agcatgaaca aatatattct tgcttactta
     4561 atttttgcaa gttgctgggt tagtaaggct attgcgtctg atttaatagt gccttattcg
     4621 cactttgcat tacgttacaa cagctcagtt aagattattc aattagcaga cagtaatttt
     4681 ttaatactta gtcgacacaa agatattggt agtactcccg caatggcagg acttacctgg
     4741 tgtcaccgga acggcgatac tatcaagaca aaattattaa ctctcaattc aggaagtgca
     4801 gcgccattag atataatttc actgtatgat ggctctcttt ttatttccgg cacaactgat
     4861 gatccgttag ataacactct tttcacaatt catctggact cgactggttc attgattagc
     4921 tttaagttgc atacttcgat tgatgcagtt tggctttcaa atagtattca attggattca
     4981 ggacatgtat tgcttgcagc aacattggtg aatgttcctg aatccattat attttataaa
     5041 gttaatcctg atggagattc cctttcattt cataattata catttcctgg gcttctcgga
     5101 gtttcttgta gtcgattatt gaatgatagt acaatacttt ttggtggtga tatgagcaat
     5161 tctaatttcg tgctccttac aaatctcaat ggcgactcac tatcaataga cacatttcct
     5221 tttacaactt ccggaaaagt tcatataagt tcaattgcca ttacaggttc tcaatatttg
     5281 gttatcgcgg gaacttcgac tgatactctt gatagtaatc accaatcggt ttggtgtatg
     5341 aagacagatt tatcaggact aatattatgg caaaatagat ttcattctgc gaaccccttc
     5401 tggaattcat ctgcaatttc cgaaatcgga acaaattatt ttgcaataaa tactttttat
     5461 aacaccacta cgacaattgg aaatcatcca cctgttttaa ttatcgattc taacggcgac
     5521 tctgtttcaa tgagtaatgc atctggcaac tattgcgaaa tggcatcggg aaataataat
     5581 tcaatcattg gagatatgaa tggcaatgct tttttaacaa cgacaatcga ttcagtttgt
     5641 tcgaacattt atcaaaacat tgcatttgag aagtttgatt tttcatccct tgtaaattca
     5701 attgatgtgc tcaataataa tcatcatcat ttggatgttt tccctaaccc tgctaacaat
     5761 catttaaatg taattgattt cgataatgaa atagggcata ttagtcttta tgatatttcc
     5821 ggtagatttc tttttagtaa aattaaagaa gaatcaattc aattgccgga tattcataat
     5881 ggggtctacg ttgtggtttt atatcataaa gatcgtaccc ctcttaaaaa aatacggtta
     5941 gtcgttttgc attaaatcat ttgtaaaagt tacttgtgag attaacaaaa gcggaagcgt
     6001 tttaaatctt tggtaaagga gtaagttact tttcagcatc tcttaccaac aatccccact
     6061 cgataacaaa atccaacttg ctaaccatcc tattgcaccc gtcaccccgc ccatgtattc
     6121 tcgcaggaaa aaagacatgg acgcattcga acaaatcatt ggccagcttc tggtagaaga
     6181 aaagtattgg gttagacatt ccgtaaagat tgatttaaca cctgaagaga aaaggtacat
     6241 caacaaacct tccacgccaa gaccagagat cgatatagta gcttataaca caacaacaga
     6301 tacaatttac ctacttgaag tgaagtctta tctggatagt ccaggtgttg tatctgagca
     6361 tgtagctatt gaacaggacg aacagtcggg ccggtacaag ctgcttaccg caatgaacta
     6421 ccggacaaca ctggcgaaac ggctgcatgc agactggtgc aaatccgggc acattcggaa
     6481 aagtacaagg atttcatttg gactcattgc cggtaaagta taccggaata gagaacttga
     6541 actacggaga tatttccaaa aacaaggttg gttgttctgg ggcccgtcgg aaatcaagag
     6601 tcgtatccta cgcctgtctc aaaagggtta tgagaataat acggtgacga ttgctgctaa
     6661 gatcttgacc agggtataag gaatctggaa cctcggttta tttgaaccaa ggttggatta
     6721 ctagtgcgaa ataaacgttc cggaaaaagg ctggtcagga attgtctggg aatggcccag
     6781 gaaggatttg gcggtgcagg gaaagcttta gattgttttg aactttatgc tgaaagaatt
     6841 gcataaaacg gcagctgata tgtttccatt aaatagttat cagttacagc taaaatgtaa
     6901 tagttagaac ttcacctgac agacagggtt ggttaaaagt tatttccggt
//
LOCUS       DAOTON010000033         6935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_9489_length_6935_cov_3.108430, whole genome shotgun sequence.
ACCESSION   DAOTON010000033 DAOTON010000000
VERSION     DAOTON010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9489_length_6935_cov_3.108430"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="P5292_01215"
     CDS             <1..1657
                     /locus_tag="P5292_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37772.1"
                     /translation="IPAPVYQRARGPVDIRIYDPVKVAPGEYVLFLQDTVTATGKWKL
                     RNVTTGQEYESEKTLEFPYDQLFPDYGFYISMNQVKSPGETPTDGNGFAEGTISYSDA
                     NSRWLSFAQDIDNFTALNWIRAGNTDGVDLTAQGEFIDGAAFFEKVLNGAWAPYRLTN
                     TDVSGGAIAPAPGYSTSAARNQAQLKYLPGVDIVFTPDKSKWSRCVVFEMQGDRNKSE
                     GRQYKNLIRKHPSLNLDGTYSTTDSGFSWFPGYAINVETGERLNIAFGEDSYLNPDSG
                     YAGQTGADMIFNPTTTLVNDSLEAIAGGRHVVYVFGHTDVELPDGVTGNDSIYKSPAY
                     DGCQYIYDLLWPITTQPLATPGRPFPKAWADCQWVGIPMAAPGATWLANTAKVRLRVT
                     RPYRQVEFDSNVTENNFFPYYKFNTYDLVSKVNQTDVAKNALDLINIVPNPYYAYSMY
                     EKNQLDNRVKITNLPSRCTISIFTPSGTLVRKFDRNVASDNTSGGVYPDLNLDSSLEW
                     DLKNTKGIPVASGMYIIHVEAPGIGERTLKWFGVMRPLDLDTF"
     gene            1696..2757
                     /locus_tag="P5292_01220"
     CDS             1696..2757
                     /locus_tag="P5292_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRS37773.1"
                     /translation="MKTRYIGSFLRVALAALLLPAGVQAGNPDRAGQAGASELLINPW
                     ARSNGWGGANSGSIRGLEAQFLNVAGTAFTKKTEAIFAHTMYLEGSGMALNAFGITQK
                     AGEAGVIGLSVVSFDFGDIPVTTTEQPEGNIGNYRPQYINIGLSYAKVFSNSIYGGFN
                     LKIINENIANVSARGIAVDAGIQYVTGTNEDRDNIKFGIALKNVGTPMKFGGDGLSVR
                     LTAPSGAYQYTVEQRTQGFEIPSLLNIGASYDFSLAKDHRLTAAANFTSNSFTRDQIA
                     GGLEYAFREMFMIRGGFVYEDGITSDDDRTTVYTGPCAGVTVDVPMGKSGKKFGIDYA
                     FRATNPFNGVHTFGLRFTL"
     gene            2840..3313
                     /gene="greA"
                     /locus_tag="P5292_01225"
     CDS             2840..3313
                     /gene="greA"
                     /locus_tag="P5292_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRS37774.1"
                     /translation="MSSVQYFTPDGLKKLQEELHQLRNVERPRISQQIAEAREKGDLS
                     ENAEYDAAKDAQGLLELRISKLEEVIASARLLDDSKLDTSKVMVLTKVRIKNRKTGQE
                     LTYTLVAENEADLKAGKISVTSPIGKGLLGKKIGDTAQIEVPSGKMEFEVLEISR"
     gene            3313..3708
                     /locus_tag="P5292_01230"
     CDS             3313..3708
                     /locus_tag="P5292_01230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRS37775.1"
                     /translation="MASIFTRIVNGEIPCYKVAEDDRFLAFLDINPLVKGHTLVIPKQ
                     ETDYIFDIQSEEYTALWAFAQRVAKAVGAAVPCKRVGIAVIGLEVPHVHIHLIPMERV
                     SDMNFANPKISLPPAVMEETAAAIRKKII"
     gene            complement(3701..4270)
                     /gene="ruvC"
                     /locus_tag="P5292_01235"
     CDS             complement(3701..4270)
                     /gene="ruvC"
                     /locus_tag="P5292_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRS37776.1"
                     /translation="MATVKERIILGIDPGTNIMGYGIIRVIGTRPELITLGVLRLKRD
                     DDHSLRLKQIFEYTLGLIDQHLPDDLAIEAPFFGKNVQSMLKLGRAQGVAMAAGLYRQ
                     VPITEYSPKRVKQSITGHGNASKEQVAAMLKTLLSLNETHAYLDATDAVAVALCHHFQ
                     SGNNSAGAAGAHSGWKSFLSANPDRIVSK"
     gene            4317..5300
                     /locus_tag="P5292_01240"
     CDS             4317..5300
                     /locus_tag="P5292_01240"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HRS37777.1"
                     /translation="MRQSTENILVRWGWILKLLIAAVAFTFIYWQVVRREGLATLVAV
                     ARDSWSEPHNRVLLSAVLLGMLINWAIEAFKWKRVLRPLEQVSLLKALEAVFSGLSVS
                     FFTPNRVGEYAGRVFHLVKADRPQAVLATILENVSQLVITLFFGGFALAYGVQDLITT
                     QSYLMSSFWILSVVGACFLVLAFIYSGVLQAMFVRLRFSGRWLRVTGILERYTKAQMI
                     ELLLLSALRYFIFTGQFFLLLHVFGAPLPYGVAMLRIALTYLAMSVIPSIALTELGVR
                     GALAAWVFQPVTEQVSGVLLATSALWVINLVLPALIGLLFVFDFKFVKKRT"
     gene            5297..6430
                     /locus_tag="P5292_01245"
     CDS             5297..6430
                     /locus_tag="P5292_01245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS37778.1"
                     /translation="MMLVLGVYTLFSFLYVIFLGRVRKAWWSLRQANVEQPASVSFPF
                     VTVIVPVRNEAQHLPDLLNDLLAQDYPDDRWELIVVNDGSADGTAAIAADFFHSHPNR
                     GGLLNLPDREGGSKKQAVAMAIANAKGEWILTTDGDCRVGPSWLTTMAQGMSPGRTML
                     AGPVNLVGSPDFLGRLQTAEWIGLQCIGAAGIYRGRNFFCNGANLAYRKQAFTESGGF
                     TRSKKRLSGDDTDLLLQFSRSWPGSIGFQSGTGAIVETFADDGIGSAFSQRHRWASKI
                     PVALTTVTLLISLIAWLSHAGFLAALCIMFFSPIAAFVVLPVFFLKVLAEYLLLKDGA
                     DYFGHRVSFGLILAAQPFYALYITLVGAAAVFLPYTWKGRKGR"
     gene            6434..>6935
                     /locus_tag="P5292_01250"
     CDS             6434..>6935
                     /locus_tag="P5292_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37779.1"
                     /translation="MRYDSPGQQVWRRFRKNGAAMSGLAVIALAILVALCCTVIRSDE
                     SPEANRICLPIGNQRPGFTCDFLLLRKNQEAMVPGLWQQFWNGTPDSMQWVAVSDWRI
                     ASDTVFYHEWSPDALLPEQFVLLAEAVWPILDPHQLVNSDGKWSARDVYGAIRTSSTK
                     DLRTTFS"
BASE COUNT         1480 a         1906 c         1900 g         1649 t
ORIGIN      
        1 tattccggct ccggtttacc agcgtgcgcg cggaccggtt gatatccgga tctatgaccc
       61 ggtcaaggtt gctcccggtg agtatgtcct gttccttcag gatacggtta ccgctaccgg
      121 aaaatggaaa ctgcgtaatg tgaccactgg ccaagagtat gaatcagaga agacactcga
      181 gttcccctac gaccagttgt tccccgacta tggcttctac atcagcatga accaggtcaa
      241 gtcgcccggc gaaacgccga ccgacggaaa cggttttgcg gagggaacga tttcctattc
      301 ggacgcgaac agccgttggt tgtcgttcgc tcaggacatc gataacttca ccgcattgaa
      361 ctggatccgt gccggcaata cggatggtgt ggatctgacc gcgcaaggtg agttcatcga
      421 cggtgccgca ttctttgaaa aagtattgaa cggcgcctgg gctccttatc gtttgacgaa
      481 tacggacgtt tccggcggtg cgatcgctcc ggctcccgga tacagcacca gtgctgcgcg
      541 taaccaggct caactgaagt atctgccggg cgttgacatc gtcttcactc cggacaagag
      601 caagtggagc cgttgcgtgg tattcgaaat gcagggcgac cgcaataaat cggaaggccg
      661 tcagtacaag aacctgattc gcaagcatcc ttcgctcaat ctcgacggaa cctacagcac
      721 gaccgattcc ggtttctcct ggttccccgg ttatgccatc aatgtggaaa ccggcgagcg
      781 gttgaatatc gccttcggag aagattccta cctcaatccg gatagcggat atgccggcca
      841 gacgggcgcg gacatgatat tcaatccgac aacaacgctg gtcaacgaca gcctggaagc
      901 catcgccggt ggccgtcacg tagtgtatgt cttcggccat accgatgtgg aactgccgga
      961 cggtgttacc ggtaacgact cgatttacaa gagcccggcc tatgatggtt gccaatacat
     1021 ctatgatctg ttgtggccga tcacgacgca gccgctggct actccgggtc gccccttccc
     1081 gaaagcctgg gctgattgtc agtgggtggg cattccgatg gctgcgccgg gcgcaacctg
     1141 gctcgccaat acggcaaaag tgcgactgcg tgtaacgcgt ccttatcgcc aggtggaatt
     1201 tgattcgaac gttacggaga acaacttctt cccgtattac aagttcaata cctacgacct
     1261 ggtcagcaag gtgaatcaga ccgatgtcgc caagaacgcg ctggatctga tcaacatcgt
     1321 accgaatcca tactatgctt attcgatgta cgagaagaat cagttggaca atcgggtcaa
     1381 gatcaccaac ctgccgtctc gctgtaccat ttcgatcttc accccgtcgg gtaccctcgt
     1441 tcggaaattc gatcgcaatg ttgcatcgga caacacctcg ggcggtgtct atcccgacct
     1501 gaatctggac agctccctgg agtgggacct taagaatacc aaaggtatcc cggttgccag
     1561 tggaatgtat attatccacg tcgaggctcc cggaatcgga gagcgtacgc tgaagtggtt
     1621 cggcgtaatg cgtccgcttg atcttgatac cttctgattc cacttcacac gcaccgacac
     1681 agaaaaacac gcactatgaa aactcgctat atcggatcgt tccttcgcgt cgcgctggct
     1741 gctctcttgc ttccggcagg cgtgcaggcg ggtaaccccg accgtgccgg ccaggctggc
     1801 gcatcggagc tcctgatcaa tccctgggca cgctcgaatg gatggggcgg agccaactcg
     1861 ggctccatcc gcggcttgga agcacagttc ctcaacgttg caggaaccgc gttcaccaag
     1921 aagacggagg caatctttgc ccataccatg tacctggagg gaagtggaat ggcgctgaat
     1981 gcgtttggca tcacgcaaaa agcaggcgaa gccggcgtca tcggattgtc cgtcgtttcg
     2041 tttgatttcg gcgatattcc ggttacgacg accgaacagc cggaaggtaa tatcggtaac
     2101 taccgtccgc agtatatcaa catcgggctt tcgtatgcaa aggtattctc caactcgatc
     2161 tacggtggct tcaacctgaa gatcatcaac gagaacatcg ccaacgtaag tgctcgtggc
     2221 atcgcggttg acgccggcat ccagtatgtt accggtacca acgaagaccg cgacaacatt
     2281 aagttcggca tcgccctgaa gaatgtgggt acaccgatga aattcggcgg cgacggtctt
     2341 tccgttcgcc tgacagcccc gtccggcgca taccagtaca cggtcgagca gcgcacacaa
     2401 ggattcgaga tcccttcgct cctgaatatt ggcgcgagct atgatttcag cctcgcaaaa
     2461 gatcatcgcc ttaccgctgc cgctaacttc acctccaact ctttcacgcg tgaccagatc
     2521 gccggtggac ttgaatatgc cttccgcgag atgttcatga ttcgcggtgg ttttgtttac
     2581 gaagacggca tcacctccga cgacgatcgt accactgttt acaccggtcc ttgtgctggt
     2641 gtgacggtgg atgtgccgat gggaaagtcg ggtaagaagt tcggtatcga ctacgcgttc
     2701 cgggctacca accccttcaa cggggtgcat accttcggct tgcgttttac gctctaaaaa
     2761 accgtatatt tgcataatcg gacaagaccc cgcaccagcg gggtcttgtt ctgctttaaa
     2821 tgccttaata gattctgtta tgtcatccgt acaatatttc acacccgacg gcctgaagaa
     2881 actacaggaa gagctgcacc agctcaggaa cgtcgaacgc ccccgcattt cccagcagat
     2941 cgccgaagca cgcgaaaagg gagatctttc cgaaaacgcg gagtatgatg ctgccaaaga
     3001 cgcgcaggga cttttggaat tgcgcatttc caaactggaa gaagtgatcg ccagcgcgcg
     3061 cctgctggac gacagcaagc tagatacttc caaggtcatg gtattgacca aagtgcgcat
     3121 caagaaccgg aagaccggtc aggaactcac ctatacgctg gttgcggaaa atgaggcgga
     3181 ccttaaggcc gggaagatat ccgtcacgtc tccgatcggc aaaggcttgt tgggtaagaa
     3241 gatcggcgac acggcccaga tcgaagtccc ttccggcaag atggagtttg aagtactcga
     3301 gatctcccgc taatggcatc cattttcacc cgcattgtca acggcgaaat accttgctac
     3361 aaggtagcgg aggatgatcg tttcctggct tttctggaca tcaatccgtt ggtgaaaggg
     3421 cacaccctgg tcattccgaa acaggagacc gattacattt tcgatattca atcggaagag
     3481 tacaccgccc tctgggcctt tgcgcagcgc gtcgccaaag cggtcggcgc cgctgtgccc
     3541 tgcaagcggg tcggcattgc tgtgattggc ttggaagtgc cgcacgtgca cattcatcta
     3601 attccgatgg agcgggtttc agacatgaac ttcgcgaacc cgaaaatcag tctgcctccc
     3661 gctgtcatgg aggagaccgc cgctgccatt cggaaaaaga tcatttagat acgatcctgt
     3721 caggattagc cgacagaaac gatttccagc cggagtgtgc accagccgct ccggctgaat
     3781 tgtttccgct ttggaaatga tggcacaggg cgactgctac ggcatccgtt gcatccagat
     3841 acgcgtgtgt ttcgttaagc gagaggagcg tcttcagcat ggcggctacc tgttcctttg
     3901 acgcgttgcc atgccctgta atggattgtt tgacccgttt aggcgaatat tcggtaatcg
     3961 gaacttgtcg gtacagcccg gcagccatcg ctacgccctg tgctcgtccc aacttcaaca
     4021 tggactgcac gtttttaccg aagaacggcg cttcaatcgc gagatcatcc ggcaaatgct
     4081 ggtcgatcag gcccagggta tattcgaata tttgtttaag ccgtagggaa tggtcgtcat
     4141 cgcgcttgag gcgcaagaca cccagggtga tcaattccgg tctggtcccg atcacccgga
     4201 tgatcccgta gcccatgatg ttggtgcccg ggtcgatccc aagaatgatt cgttccttca
     4261 ccgtagccat cttcaggggt ttccggattc aaattgcgta atattgtcgt gaccggatgc
     4321 gacaaagtac ggaaaatata ctcgttcgct ggggctggat actcaagctc ctgatcgccg
     4381 ctgtcgcctt tacgtttatt tactggcagg tggttcggcg ggaaggactg gcaacactgg
     4441 tagctgttgc acgcgattcc tggagtgagc cgcacaaccg ggtactcctc tcggccgtgt
     4501 tgctcggcat gctgatcaac tgggcgatcg aggcgttcaa atggaaacgg gtcttgcgcc
     4561 cattggagca ggtctcgctc ctgaaggcgt tggaagcggt cttctccgga ctctccgtca
     4621 gcttttttac cccgaataga gtaggggaat acgcgggtcg cgttttccat ctcgtgaaag
     4681 ccgatcgccc acaggcggtt ttggcaacca tattggaaaa cgtgagccag ctcgtcatca
     4741 cgctcttctt tggcggtttc gcgttggctt acggtgtgca ggacctgatc acgacccaat
     4801 cctatctgat gagttcattc tggatcttgt ccgtggtagg tgcctgcttc ctcgtactag
     4861 cgtttatcta ttccggtgta ttgcaggcaa tgttcgtgcg gctgcgtttc tcgggcaggt
     4921 ggctgcgcgt caccgggatt ctggagcgct ataccaaggc gcagatgatc gaactcttgt
     4981 tgctctcggc cttacgttat ttcatcttca ccggacaatt cttcctgctc ctgcacgtgt
     5041 tcggtgcccc cttaccgtat ggggtcgcga tgctccggat cgccctgacg tacctggcca
     5101 tgtcggtcat tccttcgatc gcgctgacgg aactcggtgt acgaggcgcc ttggccgcct
     5161 gggtctttca gccggtcacc gagcaggtca gcggcgtact gctcgcgaca tccgcgctat
     5221 gggtcatcaa cctggtactg cccgcgctca tcggcctctt gttcgtgttt gatttcaaat
     5281 tcgtgaagaa gcgtacatga tgctcgtgct cggagtttat acactcttca gcttcctgta
     5341 tgttatcttt ctaggtcgtg tccggaaagc ctggtggagc ctgagacaag ccaatgtcga
     5401 gcagccagct tccgtttcat tcccattcgt cactgttatt gtaccggtgc ggaatgaagc
     5461 gcaacacctg cccgatcttt tgaacgactt gttggcacag gattatccgg acgatcggtg
     5521 ggagctcatc gttgtgaatg atgggtctgc cgacggcaca gcagccattg cggctgattt
     5581 ttttcattcc cacccgaacc gcggcggtct gctgaatctt ccggataggg aaggtggatc
     5641 aaaaaagcag gctgtcgcga tggcgatagc aaatgcaaaa ggcgaatgga tcctcaccac
     5701 cgacggggat tgtagggttg gcccttcctg gttaacaact atggcgcagg gtatgtcgcc
     5761 cggtcgtacc atgctggcag gacccgtcaa cctggttggt tcaccggatt tcctgggcag
     5821 actgcaaacc gctgagtgga tcggtcttca atgcatcggt gctgccggca tctaccgcgg
     5881 aaggaatttc ttctgtaacg gagccaacct ggcttatcgg aaacaagcat tcacagagag
     5941 cggaggattc accaggagca agaagaggct gtcgggtgat gacacggacc tcctgctgca
     6001 attcagtcga agctggcccg gcagcatcgg ctttcaatcc gggacaggtg ctatcgtcga
     6061 aaccttcgca gacgatggta ttggttctgc gtttagtcag cgtcatcgct gggcatccaa
     6121 gatcccggtt gcactgacca ccgttacctt gctgatttcc ctgatcgcct ggttgtcgca
     6181 tgccggcttc ctggctgcgc tctgcatcat gtttttctcg cccattgcag cctttgtggt
     6241 gctgccggtc tttttcctca aggtccttgc tgaatacctc ttgttgaaag atggagccga
     6301 ctacttcggt catcgcgttt ccttcggctt aattttagcc gctcaaccgt tctatgcgct
     6361 ctacatcacc ctggtaggcg cggcggcagt gtttcttccc tatacctgga aaggccgaaa
     6421 aggccggtaa cccatgcgat acgattctcc cggtcagcaa gtctggcgcc gctttcgaaa
     6481 aaacggtgcg gccatgtccg gattagcggt catcgcgctt gcgatcctgg tagctctttg
     6541 ctgcactgtc attcgatccg atgaaagccc ggaggctaac cggatctgcc tgccgatcgg
     6601 gaatcaacgc ccgggattca cttgcgattt tctcttgtta agaaagaacc aggaagccat
     6661 ggtcccgggt ctgtggcaac agttctggaa cggaaccccg gattcgatgc aatgggtggc
     6721 ggtttccgat tggcgcatcg cgtccgatac ggtattctat cacgaatggt cgccggatgc
     6781 cctgctcccg gagcagttcg tgcttctggc cgaagcggta tggcccatac tggatccgca
     6841 tcagttggtg aacagtgacg gcaagtggtc agcgcgcgat gtttatgggg cgatccgtac
     6901 ttcctctacg aaagatttga gaacgacatt cagcg
//
LOCUS       DAOTON010000034         6571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_10093_length_6571_cov_3.659454, whole genome shotgun sequence.
ACCESSION   DAOTON010000034 DAOTON010000000
VERSION     DAOTON010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10093_length_6571_cov_3.659454"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="P5292_01255"
     CDS             complement(<1..1625)
                     /locus_tag="P5292_01255"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRS37780.1"
                     /translation="MEISKTYAPSQVEDKWYRYWMDKGFFRSIPDEREPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDVLIRRARMLGFNACWVPGTDHASIATEAKVVAMLREKGIKK
                     SDLSRDEFLKHAWEWKEKYGGIILEQLKKLGASCDWDRTRFTMEDDLSDAVISVFIDL
                     YNKGHIYRGVRMVNWDPQGKTALSDEEVIHKEVNSRLYHVRYPVIDAALAEELKSSNS
                     AWTIADDGWQVTVNGRTHNGDGFITIATVRPETILGDTGVCVHPDDPRYTHLRGMFAV
                     IPMVNRPVPFIFDDYIDMEFGTGALKVTPAHDINDYNLGVKYGLEVIDTINDDGTMSA
                     AARFYVGEDRFVVRKKIAKDLEASGHLVKAEDYRNKVGYSERTDAVIEPKLSMQWFLR
                     MEQVSKPALDNVLNGNIQLIPEKFINTYRHWMENVRDWCISRQLWWGQRIPAWYSPDG
                     TDFVVCKTETEAIRLFETKGKKFGTGQLRQDEDVLDTWFSSWLWPISVFDGFKDPDNK
                     DIQYYYPTNDLVTAPEILFFWVARMIIAGYEYRG"
     gene            complement(1797..2174)
                     /locus_tag="P5292_01260"
     CDS             complement(1797..2174)
                     /locus_tag="P5292_01260"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRS37781.1"
                     /translation="MIIHAPYLPAGVWLYVFGLGVLLGLWAVIVIPPQSLRIHPEPAT
                     DAKLVRRGPYRVIRHPMYAALLLVGISWASDHPSFSRWMLFVMLLLVLVTKIKLEERI
                     LSNRFAAYQEYQRSSWKLVPFIW"
     gene            complement(2233..3915)
                     /locus_tag="P5292_01265"
     CDS             complement(2233..3915)
                     /locus_tag="P5292_01265"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRS37782.1"
                     /translation="MVVVLLLSSCHRHTEEKARSVPDTTGLAVLKKTDLFTDSSFVES
                     WLGAQRVDSLARIATREFYYQRYYRFAWFTPEGPSERARAFLNTIQQAAASATTEDTA
                     SWSAMTVYADSLLSSWSPDSIDASRDIGMTALYFSYTHREFEGLPEEDLQRLEWFIPR
                     KKVDWVQMLDSSIRSAGVAVPWQGPMFHQYDRLIRVLASYRSIRASGGWQLVDPVVLM
                     ARVGDADSSIAQLRKRLLQSDDLQDTSTSDLYDDALALAIKRFRLRHGLNGEAKLDSA
                     TLRLLNLPVDSGIRTILLNLERCRWVPEDPIGRHLVVNIPAFRLYAFSDSGFAWSTRV
                     IVGKVGTATTIFTGSIRTLVLNPTWTVPRKIVYEEIIPAQRANADYLTRNKMDLFRPS
                     DLSHPVNPKKVDWKKAGPGSFPYIVRQRSGSWNALGRYKFLFPNSYDIYLHDTPSRQL
                     FERSERTFSHGCIRVAQPERLARFVLSGDSLWTEERLAGELDLGKERFIPLLNKVPVS
                     IVYFTAWVDEDGILQEREDIYGHDRKLADVLFKSTDFAPSDSNLVLVNESRE"
     gene            complement(4044..4694)
                     /locus_tag="P5292_01270"
     CDS             complement(4044..4694)
                     /locus_tag="P5292_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRS37783.1"
                     /translation="MKSFLLACITSIALLLGTSYKTVSAQNTSSPSIGIKGGLNYSSL
                     NFSFTAWDKPKTGYHLGAFVNLPLSDRFSIQPELLYSTKGTRVPYRITDWLDGQVALD
                     LRYLDLPVMGQVKLGRFAHIEAGPYASLLLDANLKHGSTIPFLNFSYDLPNDVFSRLD
                     YGLAAGAGMDLGHFGVGVRYNYGLGKVEDVKQLLGTDFTITNARNTTWQLSVTYRF"
     gene            complement(4809..6131)
                     /locus_tag="P5292_01275"
     CDS             complement(4809..6131)
                     /locus_tag="P5292_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37784.1"
                     /translation="MVDFPEPFVRRMRALLGEEAESFFSALQSPAPVSLRLNPAKPVE
                     SFREEKEVAWCAEGRLLLQRPSFTQDPLFQAGCYYVQEASSMFLGSAYRQLFGARAPE
                     RVLDLCAAPGGKSTHLRSLLPAESLLVSNELIASRNQVLVQNLVKWGDPGIVVTQSDP
                     EQFRRLPDYFDLVVVDAPCSGEGLFRKDPAAIDEWSENAVEACAIRQTNILASAWEAL
                     APGGALFYSTCTYEPAENEEQVERLRKLGAEILQLQAPQGVDERSGCLRFYPHRIAGE
                     GFFFCALRKPEGSVTQRPRPGKSGIQVVQDPRYAAYLQPVDQWQAFRVGPHLHVIAQR
                     FVGALHELAAGLTIRYAGVRVGEIMGDQLIPAPELAFSTALSDTVRRIELDDAQAIRF
                     LKGEALRFNGALPGWCLVTTSGYGLGWVKVVSDGRANNHFPKGWRIRH"
     gene            6187..>6571
                     /locus_tag="P5292_01280"
     CDS             6187..>6571
                     /locus_tag="P5292_01280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS37785.1"
                     /translation="MSGYRISTDKRRLQLPVIHDFLRESYWAKDIPESVVRRSVDGSL
                     CFGVYHGREQVGFARVITDEATFAYLADVFILPAHRGKGLSRRLIEEIIAHPALQGLR
                     RMLLATCDAHELYARFGFKGLGIPDR"
BASE COUNT         1607 a         1847 c         1685 g         1432 t
ORIGIN      
        1 ccccggtact cataaccggc aatgatcatc cgcgcaaccc agaagaacag gatctccggg
       61 gcagtcacca ggtcgttggt ggggtaatag tattgaatgt ctttgttgtc gggatctttg
      121 aagccgtcga atacactgat cggccacagc caactggaga accacgtgtc caggacatcc
      181 tcatcctgcc tgagttgccc cgtcccgaat ttcttcccct tcgtttcaaa caacctgatc
      241 gcttcggttt ccgtcttaca gacgacgaaa tcggttccgt caggagagta ccaggccgga
      301 atacgttgtc cccaccacaa ctggcgcgaa atacaccagt cgcggacatt ctccatccaa
      361 tgccggtagg tattgatgaa tttttccggg atgagctgga tgttgccgtt aagcacattg
      421 tccagggccg gcttcgagac ctgctccatc cggaggaacc actgcatcga taatttcggc
      481 tcgatgacgg cgtcggtccg ctcgctgtag ccgactttat tcctgtaatc ttccgccttg
      541 acaagatgac cggatgcttc gagatccttg gcgatctttt tgcgaaccac aaaccggtct
      601 tctccgacat agaaacgagc cgcggcactc atggtaccgt cgtcgttgat ggtatcgatc
      661 acttcgagcc catacttcac acccaggttg tagtcgttga tatcgtgagc aggcgtcacc
      721 ttcagcgccc ccgtaccgaa ctccatgtcg atgtaatcat cgaagatgaa aggaaccggg
      781 cggttcacca tcggaatgac cgcgaacata ccacgcaagt gggtatagcg cgggtcatcg
      841 gggtggacgc atacgcccgt atcgcccagg atcgtctccg ggcggacggt cgcgatggta
      901 atgaatccat cgccattgtg cgtacgtcca ttgaccgtta cctgccaacc gtcgtcggca
      961 atggtccatg ctgaatttga cgatttgagt tcctccgcga gcgccgcatc gatgaccgga
     1021 taccggacat gatacaaacg cgaattcact tctttgtgaa tgacctcctc gtcgctgagt
     1081 gctgtttttc cctgcgggtc ccaattgacc attcggacac cgcgatagat atgccctttg
     1141 ttgtagaggt cgatgaaaac ggagatgacc gcgtcactca ggtcgtcctc catggtaaaa
     1201 cgcgtgcggt cccagtcgca ggaagcgccc agcttcttca actgttcgag gatgatgcca
     1261 ccgtacttct ctttccattc ccaggcatgc ttcaggaact catcacggct caggtcgctc
     1321 ttcttgattc ccttttcgcg caacatcgcc acgactttcg cttccgtcgc aatcgaagca
     1381 tggtccgttc ccggcaccca acatgcattg aagcccaaca tgcgggcgcg ccggatcagc
     1441 acgtcctgga tcgtgttgtt caacatatgc cccatgtgca aaacgcccgt tacgttgggc
     1501 ggggggatca cgatcgtata aggttcacgc tcatccggaa tactccggaa gaagccctta
     1561 tccatccagt aacggtacca cttatcttct acctgggaag gcgcgtatgt ctttgaaatt
     1621 tccattgaat gcggggagaa aggccaaatt tacgcatttt cagtgtaagg attcagccca
     1681 atgtaagacc ggaaagggag agcccgtcca ttcaggggat tcgaagattc aacctgcgtt
     1741 tacaaacaac ccattcggtt ttaacagccg gtgaaatcgt tcagaaactc gcgcaattac
     1801 cagataaatg gcacgagctt ccacgaggat cgttgatact cctgatatgc agcaaacctg
     1861 ttgctcagga tccgctcctc taacttaatt ttcgtaacca gcaccagcaa gagcattacg
     1921 aaaagcatcc atcgggagaa cgaaggatgg tctgacgccc aggaaatacc aaccaacaac
     1981 agcgccgcat acatcggatg acggatcaca cgatagggcc cccgacgaac aagcttcgca
     2041 tccgtcgcag gctcaggatg aatgcgaagg gattgcggag ggattacgat aacagcccaa
     2101 agaccgagca gtacacccaa cccgaacacg tatagccaga cgccagccgg caggtagggt
     2161 gcatgaataa tcatggcaag aagcagcgcg aactgcaaga caaccaagac ccaaccggcc
     2221 gttcgcacgt ggctattccc tgctttcatt taccaaaaca agatttgaat ccgaaggggc
     2281 gaaatcggtg gacttgaaga gaacatctgc cagcttccgg tcatggccat agatatcctc
     2341 acgttcctgg agaatgccgt cttcatccac ccaggctgta aaataaacaa tgctgaccgg
     2401 caccttattc aagagcggta tgaaacgctc cttacccaga tccaattccc cggcgagtcg
     2461 ttcctccgtc cacaaggaat ctccgctaag gacgaaacgg gccagtcgtt cgggttgtgc
     2521 tactcgtatg cagccatgac tgaaggtacg ctcggaccgc tcgaacaact gacgggatgg
     2581 cgtatcgtgc aggtaaatat cgtacgagtt tggaaagagg aacttgtaac gaccgagcgc
     2641 attccaactt cccgagcgct gtcggacgat ataagggaag cttccaggtc cggccttttt
     2701 ccagtctact ttcttcggat tcacgggatg gctcaagtcc gatgggcgga aaaggtccat
     2761 tttatttcgg gtaaggtaat ccgcattcgc ccgttgcgcc ggaatgatct cttcatacac
     2821 gatcttacgc gggactgtcc aggtcggatt cagcaccagg gttcgaatcg aaccggtaaa
     2881 gatggtcgtt gccgtgccga ctttccccac gatcacccgc gtgctccagg caaatcccga
     2941 atcactgaac gcatacaatc gaaacgcggg gatattgacg accaggtgcc gaccaatcgg
     3001 gtcttccgga acccaccggc atcgctcaag gttcagcagg atggtgcgaa taccggaatc
     3061 gactggtaag ttgagcaatc gtaaggtcgc gctatccaat ttcgcttcac cattcaggcc
     3121 atggcgtaat cgaaatcgtt ttatcgccaa agcgagagcg tcatcgtaaa gatccgacgt
     3181 actggtatcc tgcaaatcgt ctgattgaag caggcgcttt ctcaattgag caatgctgct
     3241 gtccgcatcc cctacccgcg ccattagcac aaccggatct accaattgcc atccgccgga
     3301 tgcgcgtatg gaccgatagg atgccagaac ccggatcaac cgatcatact ggtgaaacat
     3361 cggcccctgc cagggtaccg ctaccccggc gctgcgaatc gacgagtcga gcatctgcac
     3421 ccagtcaacc ttcttgcggg ggataaacca ttccagacgt tgcaggtcct cttccggcaa
     3481 cccttcgaac tcccgatggg tataggagaa atacaaggca gtcatgccga tatcccggct
     3541 cgcatcaatt gaatcaggac tccacgaaga caataggctg tccgcgtaca ccgtcatggc
     3601 cgaccatgaa gcggtgtctt cagtagttgc ggacgcagcc gcctgttgta tggtattcaa
     3661 aaaggcccgt gcacgttccg aagggccttc gggtgtgaac caggcaaatc ggtaatagcg
     3721 ttggtaataa aattcccgcg tggcgattcg tgctaaggag tcaacccgct gagcacccag
     3781 ccatgattcg acaaatgaac tatcggtaaa gagatctgtc ttctttaaga cggcgaggcc
     3841 ggtggtatcg ggtaccgaac gcgctttttc ttccgtgtgc cgatgacagg atgaaaggag
     3901 cagaacgacg accaacaagc tcaaactcga aatacctatc gtccgttgat cgtgcctgcc
     3961 aatcattatc ctgtgatata ccgggtgaaa atagcaaagt ccggagttgt ttttccggac
     4021 tttgtaacga attatcctga tgatcagaaa cgataagtaa ccgataattg ccaggtagtg
     4081 ttacgggcgt tggtgatggt gaaatccgtt cccaacagtt gcttcacgtc ttctactttc
     4141 cccaagccgt agttgtaacg aacccctacg ccaaaatgac caaggtccat cccggcacca
     4201 gcggcaagac catagtccag gcgggagaat acatcattcg gcaagtcata gctgaaattc
     4261 aggaacggga tcgtactgcc gtgtttcaaa ttggcgtcca ggagcagcga agcatacgga
     4321 ccggcttcga tgtgcgcaaa ccgaccgagt ttgacttgtc ccattacagg taaatcaaga
     4381 tagcgcagat ccagggcgac ctgaccatcg agccagtcgg tgatacggta gggaacccgg
     4441 gtacctttcg tggaatacag caactccggt tggatggaaa aacgatcact caaaggcaag
     4501 ttgacgaaag cgccgagatg atatccggtc ttgggtttgt cccatgcagt aaatgaaaaa
     4561 ttcagggagg aataattcag tccgcctttg ataccaatcg acggactgga agtattctga
     4621 gcgctgacag tcttataaga ggtgcccagc aacaaagcga tactcgtaat gcaagcaagc
     4681 aggaagcttt tcatggctgt gaggtttggt tgccgccact atgaaaatcg catgccatcc
     4741 tgctccgaag cgcatgaagc cgaagtgaag cgctggtgca atccgttgag attccaatga
     4801 atgcccgctc aatgacgtat tctccagccc ttggggaaat gattattggc gcggccatcg
     4861 ctcaccacct tcacccaacc gagtccgtat ccggaagtcg tgacaagaca ccagcctggc
     4921 aatgctccgt taaaacgcaa tgcctcaccc ttcaggaagc ggatcgcttg agcatcatcc
     4981 aattcgatcc gcctgacagt atccgacaag gccgtactga aagctagttc aggagcggga
     5041 atcaattgat cacccatgat ttccccgaca cggactcccg catagcgaat cgtaagcccg
     5101 gcagccagtt catgcaaggc gccgacaaac cgttgcgcta ttacatgcag atggggtccg
     5161 acccgaaatg cctgccactg atcgacaggc tgcaggtaag cagcataacg aggatcctga
     5221 actacttgaa ttcctgactt acctggccgt ggccgttgcg tcacggatcc ctccggtttg
     5281 cgcagcgcgc agaagaaaaa tccttcgccg gctatacgat gcggataaaa acgcagacat
     5341 ccggaacgct cgtcgactcc ctgcggtgcc tgcaactgaa ggatttccgc gcccaatttc
     5401 cgcaagcgct ccacctgttc ttcgttctcc gcgggctcat aggtacaggt cgaatagaac
     5461 aatgctcctc ccggcgccaa tgcttcccag gcggaagcta gaatgtttgt ttgcctgatc
     5521 gcgcaagcct ccaccgcgtt ttcactccat tcgtcgatgg cggcaggatc ctttcgaaac
     5581 aagccttctc cgctgcaggg cgcatcgaca accaccaggt caaaatagtc cggcaggcgc
     5641 cggaattgtt ccgggtccga ttgcgtgaca acaattcccg gatcccccca cttcacgaga
     5701 ttttgaacaa ggacctgatt gcgggaagca atgagttcat tcgaaaccag gagactttcg
     5761 gctggcagca ggctgcgcaa atgagtcgac ttccctcccg gcgccgcgca tagatcgagt
     5821 acacgttcgg gagcacgtgc accaaaaagc tgccggtatg cgctcccaag aaacatggaa
     5881 ctggcttcct gcacataata acagccggcc tggaacaagg ggtcctgcgt aaaggatggc
     5941 cgctggagga gcaaccgccc ttcagcgcac catgcaacct ctttttcttc acgaaacgac
     6001 tcaaccggct ttgccggatt cagacgcaag ctgaccggtg ccggtgattg caaggcggaa
     6061 aagaaggatt ccgcctcctc accgagtaag gcccgcatgc gacgtacaaa gggttccgga
     6121 aaatcgacca cgtgctgttg ttcctgcagc gagattatta cttttccggc aaagcgtcaa
     6181 acagtcatga gcggataccg tatcagcacc gataagcgtc gactacaact tccggtaatt
     6241 cacgatttcc tgcgcgaatc atactgggcg aaggatatcc cggaatcggt ggtacgtcgg
     6301 tcggtcgatg gctctttgtg ttttggcgtg tatcacggcc gggagcaagt cggttttgcc
     6361 cgggtcatta cagacgaagc caccttcgcc tatctcgccg atgtctttat cctgcccgcg
     6421 caccgcggga aaggcttgtc aaggcgcttg atagaagaga tcatcgccca cccggccctg
     6481 cagggacttc gtcgtatgct gctggcgaca tgcgatgcgc acgagctcta tgctcggttc
     6541 ggattcaaag gactgggaat tcccgaccgt t
//
LOCUS       DAOTON010000035         6552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_10135_length_6552_cov_3.366939, whole genome shotgun sequence.
ACCESSION   DAOTON010000035 DAOTON010000000
VERSION     DAOTON010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10135_length_6552_cov_3.366939"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="P5292_01285"
     CDS             complement(<1..395)
                     /locus_tag="P5292_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020405760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRS37786.1"
                     /translation="MSAQVTIMGLGYIGLPTAALIASKGIPVQGVDVNKKVVDTINKG
                     KIHIVEPDLEGLVHYAVQKGLLVASTSVKSSDVFLVAVPTPFKGDHEPDLAYVEKATR
                     MILPQLRAGNLFIIESTSPVGTTEKMAKL"
     gene            complement(431..1555)
                     /gene="wecB"
                     /locus_tag="P5292_01290"
     CDS             complement(431..1555)
                     /gene="wecB"
                     /locus_tag="P5292_01290"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRS37787.1"
                     /translation="MSPGKRLLFIFGTRPEAIKLAPLIREFQWRPGFTVKVAVTAQHR
                     EMLDQVLQFFSIRPDYDLNIMRPNQTLFDVTADGLKALQQVFDDAKPDLVFVQGDTTT
                     SFIGALAAYYKKVKVAHVEAGLRSGDKYAPYPEEMNRIMAGHLADIHFTPTEQSNRNL
                     AAEGITQQVFNVGNTVIDALFLGLDLLRPKGEAFFRAAFNGVNLDRKLVLITGHRRES
                     FGAPFEQICHAIRNTAIAHPDVEFVYPVHLNPNVQDPVNRILQGLENVHLISPLDYPH
                     FIWLMNRSSVVLTDSGGLQEEAPALGKPVLVMRNVTERQEGVDAGTARLVGTDRELIE
                     TELHKLLTNQTEYERMAKAVNPYGDGTACRKIVEKVNELI"
     gene            complement(1618..2664)
                     /locus_tag="P5292_01295"
     CDS             complement(1618..2664)
                     /locus_tag="P5292_01295"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRS37788.1"
                     /translation="MFQSFLTALVLALAAMPVLIRIAQQRGFVDLPDQRKLHQRPIPL
                     LGGLGIVSSFLLAVLFWSAPWFVPEQLFLLTALLVVFLMGLRDDLLPLGPQLKLAGQG
                     VAALIVVVPAGYRLHDLNGLLGMHLLDGGLSVALSVLFVLFVMNAFNFIDGADGLAAL
                     LSGMASIAFVAIFSLAGDQLFALMAASLAGALLGFLPFNMQPARIFMGDAGSLPVGLL
                     LAVFSIRMLALSPDLDFLPAGQLPSILLGLLIVPVVDLLRVITLRVFAGKSPLSPDRY
                     HIHYRLLELGWSHRRVSLTLFVVNLGFLSFTVFASGIGSPSQTFLITLLMGLLFSQLP
                     YWLLRRKISDSALK"
     gene            complement(2735..4333)
                     /locus_tag="P5292_01300"
     CDS             complement(2735..4333)
                     /locus_tag="P5292_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37789.1"
                     /translation="MRLRFLLAFIPLYVSLLPLKAQEYLSPMHRDWAIQTGGFLSVDT
                     TGFYTSVRPYMACELRNIMPYDSLRQYHGRDARWLSTWFGRKLRKEHLFQVRQDVFRL
                     DIDPIVNLRMGREQEQGRNVFTNTRGVMVQGDYKDKFYFYTRFYENQERFVNYIDSLV
                     RRDSVVPGQGKVKFLESETFDFSQAVGGLGYRLNEHFDFLLATDKNFLGDGYRSLLLS
                     DNAYSYPFFRAQMSFWKFRYAVYYTVLQNMREPHDDNSGYFRKYATIHYLSVNVGRKN
                     RLNLSLFEGVLWKPAAYRGFELHYLNPMLFLRPVENSLDSPDNALLGLNVRWNIHKGH
                     VVYGQLLLDEFLLDEVRAGKGWWGNKQGLQFGYKGFDLFGVPALGFQSEINFVRPFTY
                     QHRTNFQDYTHANQPLAHPLGADFTELLAFANYRWRSFGVEVRFQYAVTGQDTGTSNL
                     GNDIFKSYETRQGEYGNRMYQGLRTNLTAFTGKLTYCINPATGLVLEAGLTQRERSND
                     DGTQATQLVFFGLRTSLDQHYFDF"
     gene            complement(4351..5211)
                     /locus_tag="P5292_01305"
     CDS             complement(4351..5211)
                     /locus_tag="P5292_01305"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRS37790.1"
                     /translation="MRLLTFDLEDWYHILDHPETERPEQWISFPSRIERNTERILNWL
                     RERKVRSTWFVLGWVAERYPELVRRIAAEHDIAAHGYAHQLVYRSDRESFREDTRRCL
                     ECIRQAIGRDVSVYRAGGFSVTKATPWFFEELAHAGIEIDASIFPAARNHGGLPGAQW
                     SKPFIVQGDGWQIREFPLMYQTIAGRRLVFSGGGYFRLLPYPLIRRFTARADYTMTYF
                     HPRDFDPDQPVLSSLPVKRQWMSYTGLRSAPAKFRQYILDFEFTSIETADRTIDWSDR
                     PVFRHEELFR"
     gene            complement(5220..6290)
                     /locus_tag="P5292_01310"
     CDS             complement(5220..6290)
                     /locus_tag="P5292_01310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS37791.1"
                     /translation="MNADQIARWRVGDSLAPLLRLAATTYPGQELTSEDYLDWQYRRN
                     PDGPVLLHLGQSDQRVAAQYLVIPHTYWINGAPVKGSLSINTLTDPMHRGEGWFVRLA
                     EATYLACQQEGLTFTIGFPNRQSHSGFVGRLEFRSLGNLEFLAMPLRWSGIARRLVSR
                     TRKRKGSDLPIALPSPCLYRSKEYCIEEWEPGALSETFFRHFHESKLATTHRTSAYLM
                     WRYQQCPTRHYRFLRITDPGRTHMLGFVVIRALELSGLRSGVLVDFGCLPDTMSALAE
                     LFRFAIGRFQASGLETVVAAAVPASEEADQLIAEGFYRVPVRFLPQPLNMILRTHTPQ
                     SSSGIPTDLRSWFLTFGDYDVL"
     gene            complement(6287..>6552)
                     /locus_tag="P5292_01315"
     CDS             complement(6287..>6552)
                     /locus_tag="P5292_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRS37792.1"
                     /translation="NPALVEAMATAAVCLCHDNPFNRETTAGAAIYFSSDKELSSKLI
                     ELEQQSAGQRQAARLQMLQLAQERYSWERIVDQYLELFELAGR"
BASE COUNT         1668 a         1792 c         1709 g         1383 t
ORIGIN      
        1 atgagctttg ccatcttctc cgtcgtcccg accggactgg tggattcgat gatgaacagg
       61 ttcccggctc gcaactgcgg caggatcata cgggttgctt tctcgacata ggcgaggtcg
      121 ggctcgtggt cgcctttgaa aggcgtgggt actgcgacga gaaacacatc ggagctcttc
      181 acactggtac tggcaaccag caaacccttt tgtacggcat aatgcacaag gccttcgagg
      241 tcgggttcca cgatgtggat ctttcctttg ttgatcgtat cgacgacctt tttgttgaca
      301 tccacccctt gcaccggaat tcccttgctc gcgatcaaag ccgcggtcgg aagtccgatg
      361 taccccaaac ccatgattgt tacctgcgca gacatgctat tccttcaata ggatccggag
      421 accccgttta tcaaatcaat tcgtttactt tttccacgat cttcctgcag gccgtgccgt
      481 ctccgtaagg attgacagct ttggccatgc gttcatactc cgtttgatta gtgaggagtt
      541 tgtgcaattc cgtttcgatc aattcacggt cagtacccac cagtcgggcc gtacccgcat
      601 ccacaccttc ctgccgttcg gtgacgttgc gcatcaccag gaccggtttc ccaagcgccg
      661 gagcctcttc ctgaagtccg cccgaatcgg tcagcacgac cgacgaacgg ttcatcaacc
      721 agatgaagtg cggataatcg agcgggctga tcaggtgaac attctccaat ccttgcagga
      781 tcctgttgac cgggtcctgc acattgggat tgaggtgtac cgggtaaacg aattccacat
      841 ccggatgggc gatcgcggta ttccggatcg catggcagat ctgctcaaag ggtgcgccga
      901 aactctcccg gcggtggccg gtgattaaaa ccaacttccg gtcaaggttg accccgttaa
      961 aggcggcacg aaaaaacgct tcgcctttcg gtcgcaagag atcaagaccc agaaacaaag
     1021 catcgatcac ggtattccct acgttgaaaa cctgttgggt gatcccctcc gccgccaggt
     1081 tccggttcga ttgctccgtt ggtgtgaagt gaatatccgc cagatgtccc gccatgatcc
     1141 tgttcatttc ttcaggatag ggcgcatact tatccccact gcgcagaccc gcttccacgt
     1201 gagcgacctt cacctttttg taataagcgg ccaaggctcc aatgaaagaa gtcgtggtat
     1261 cgccttgtac aaatacaagg tccggtttcg catcatcgaa aacctgttgc aaggccttca
     1321 ggccatcggc tgttacgtcg aacagggtct gattcggccg catgatgttc aagtcatagt
     1381 cgggtcggat cgagaagaat tgcaacacct ggtccagcat ttcccggtgc tgcgccgtca
     1441 cggcaacctt gacggtgaac ccgggtcgcc attgaaattc acggatcagg ggagccaatt
     1501 tgatggcttc cggacgagtt ccgaagatga ataacaggcg tttaccgggt gacatgagta
     1561 gattccggac ctaagtccgg gacttacctt ataccaaaag caacggtcag ggttgcacta
     1621 tttcagtgca gaatcgctga ttttacgccg caaaagccag tacggaagct gagaaaagag
     1681 caatcccatc agaagggtaa tcagaaaggt ttgagatggc gacccgattc ccgaagcgaa
     1741 cacggtgaag gataaaaagc ccaggttcac cacgaacaag gtcaggctga cacgccgatg
     1801 tgaccaaccg agttccagca atcggtaatg gatatggtaa cgatccggtg aaaggggcga
     1861 ctttccggca aacacgcgca aggtgattac ccgaagcagg tcaacgaccg gtacaatcaa
     1921 caagcccagg agaatcgaag gcagctggcc ggccgggaga aaatccaggt caggtgataa
     1981 agcaagcatc cgaatggaaa aaaccgcgag cagaagccca accggtaaag agccggcgtc
     2041 ccccataaag atcctggcgg gttgcatgtt gaacggaagg aaaccaagca gtgcgccggc
     2101 taaggacgca gccatcagcg cgaataattg atcacctgcc agggagaaga tggctacgaa
     2161 tgcaatcgag gccatgccgc tcagcagtgc tgctaaacca tccgcgccgt cgatgaagtt
     2221 aaaggcgttc atcacaaaaa ggacaaagag gacggacaat gcaacggaca gtccgccatc
     2281 caacagatgc atccccaaca agccattcaa gtcatgcaac cggtaaccgg caggtacgac
     2341 tacgatcaag gctgcaacac cctggccggc gagtttgagc tgtggcccga gcggcagcag
     2401 gtcatcgcgc agtcccatca ggaaaaccac caacaaggct gttagcagga aaagttgttc
     2461 agggacaaac cagggagcag accagaacaa tacggcaagg agaaacgagg agacaatgcc
     2521 aaggccgccc aggaggggaa tgggccgctg atgcagtttt cgctggtcgg gcagatcaac
     2581 aaatccacgt tgctgcgcga tccggatcag gaccggcatg gcagccagcg caaggaccaa
     2641 cgctgtcagg aaagactgaa acacgaccat tcgggatacg aaggtaaagc aacggaatca
     2701 aagtggatgt taaagtgaaa agtaccggac aacactaaaa atcgaagtag tgctgatcca
     2761 ggctggtacg caaaccgaag aataccaatt gtgttgcctg tgtgccgtcg tcgttggatc
     2821 gttcccgttg ggtcagtccg gcttcaagca ccaatcccgt tgcggggttg atgcaatagg
     2881 tcagcttccc ggtgaaggcg gtgaggttgg tgcgcaggcc ctggtacatg cggttcccat
     2941 attcaccctg acgcgtctca taacttttga aaatgtcgtt acccaaatta ctggttccgg
     3001 tatcctgtcc ggtaaccgcg tattggaacc gcacttcaac tccgaaagaa cgccagcggt
     3061 aattggcaaa agccagcaac tccgtaaagt cagctcccaa cggatgggcc agcggctggt
     3121 ttgcatgcgt gtaatcctga aaattcgttc gatgctggta cgtgaacggg cgcacaaaat
     3181 tgatctccga ctgaaatccc agtgcaggaa cgccgaacag atcgaatccc ttataaccga
     3241 attgaagtcc ctgtttgttt ccccaccatc ccttgccggc cctcacctcg tccaacagga
     3301 attcgtcgag gagcaactgc ccgtagacga catggccctt gtggatattc caacggacat
     3361 tgagacccag cagtgcattg tcaggtgaat ccagcgaatt ctccacaggg cgcaggaaga
     3421 gcatcggatt caggtaatgc agttcaaatc cgcgatatgc cgcaggcttc cataagacac
     3481 cctcaaacaa actcaggttc aaacgattct tccgaccgac attgacactc aggtagtgga
     3541 tggtagcgta ctttcgaaag tatcccgaat tgtcgtcgtg cggttcccgc atattctgca
     3601 agacggtata ataaacggca taacggaatt tccaaaaaga catttgggca cgaaaaaacg
     3661 gataactgta ggcattgtct gacaacaaca acgaacgata accatcaccc agaaagttct
     3721 tatcggtagc cagcaggaaa tcgaaatgct cgttcaaccg ataaccgagg ccgccaaccg
     3781 cctgactaaa atcaaatgtt tccgattcga ggaacttcac ttttccctga cccggcacaa
     3841 ccgagtcccg ccttaccaga ctgtcgatat aattcacgaa tcgctcctga ttctcgtaga
     3901 agcgcgtgta gaaatagaat ttgtccttat agtcgccttg caccatcaca ccacgcgtgt
     3961 tggtgaatac atttcggccc tgctcctgtt cccgtcccat tcgaaggttg acaatcggat
     4021 caatgtccaa tcgaaaaaca tcttgccgta cctggaatag atgttccttc cggagcttgc
     4081 gcccgaacca ggtcgatagc catcgcgcat ccctgccatg atactgccgc aatgaatcat
     4141 acggcatgat gttccgaagc tcgcatgcca tatatggcct gacggaagta tagaacccgg
     4201 tagtgtcaac ggaaaggaac cctccggtct gtatagccca atcccggtgc atgggtgata
     4261 aatactcctg tgccttcagc gggagcaggc ttacatagag cgggataaaa gccaaaagga
     4321 atcgcaggcg catgctgtaa ggtagataaa tcaacggaaa agttcctcgt gccggaagac
     4381 gggccgatcc gaccaatcga tcgtccggtc agctgtttcg atggatgtga attcgaagtc
     4441 cagtatgtac tgcctgaatt tcgccggtgc cgagcgtaag cccgtatagg acatccactg
     4501 gcgctttacc ggcaatgaag acaataccgg ctggtccgga tcgaaatcgc gcggatggaa
     4561 ataggtcatc gtataatcgg cacgcgcagt aaatcgtcgg atcagcggat acggcaataa
     4621 ccggaagtat ccacctccgc tgaatactaa tctacgacct gctatggttt gatacatcag
     4681 cggaaactcc cggatctgcc aaccgtctcc ctgaacgata aacggcttgc tccattgcgc
     4741 tcccggaagt ccgccatgat tgcgggcagc cgggaagatc gatgcatcga tttcaattcc
     4801 cgcatgagca agctcttcga aaaaccaggg cgtggctttc gtaacagaga agcctccggc
     4861 ccgatagacc gatacgtcac gaccgatggc ctgtcggatg cattccagac acctgcgtgt
     4921 atcctcgcga aaactttcac gatcggacct gtacactaac tggtgggcat atccgtgtgc
     4981 ggctatatca tgttccgcag cgatcctacg gaccagttcc ggatatcgtt ccgccaccca
     5041 gcccagcacg aaccaggttg aacggacctt cctttcacgc aaccagttca ggatgcgctc
     5101 ggtattccgc tcgatgcggg atgggaagga gatccattgt tccggtcgtt ccgtttccgg
     5161 atggtccagg atgtggtacc agtcttccag atcgaatgtc agtagccgca acgagggttt
     5221 cacaagacgt cgtagtcccc aaaagtaaga aaccacgacc gcaagtccgt cggaatccca
     5281 gaactggact gaggggtgtg ggtacgcagg atcatattca ggggttgagg taaaaatcgt
     5341 accggcacac gataaaatcc ttcagcgatc agttgatctg cttcctccga tgcagggacg
     5401 gctgcagcga caacggtttc gagacccgat gcctgaaatc gtccgatggc aaaacggaat
     5461 agctctgcca gcgccgacat cgtatcgggc aggcatccga agtctaccaa cacgccgctg
     5521 cgcaagccgg acaattcgag tgcgcgtatg acgacgaacc ccagcatatg tgtccgacca
     5581 ggatcggtga tgcggagaaa acgatagtgg cgcgtcgggc actgttggta ccgccacatt
     5641 aaataagccg atgtgcggtg ggtggtcgcc agtttgcttt catgaaaatg ccggaagaac
     5701 gtttcggaca atgcccccgg ttcccattct tcaatacagt attctttgct ccggtagaga
     5761 caaggagacg gtaaggcaat gggaaggtcg gaacccttcc gctttctggt acgacttacc
     5821 aatcgcctgg cgatacccga ccatcgcaag ggcattgcca gaaattcgag attgcccaga
     5881 gaccggaatt ccaatcgtcc gacaaagccc gaatgcgatt gcctgttcgg aaaaccaatg
     5941 gtgaatgtca gtccctcctg ttgacaggcc aaataggttg cttctgcgag ccgaacaaac
     6001 caaccttcgc cccgatgcat cggatccgtc agcgtattga tcgatagcga tccctttaca
     6061 ggtgctccgt tgatccaata agtatgcgga ataaccagat actgagctgc gacgcgttga
     6121 tccgactgcc caagatgcag cagaactggg ccatccggat tcctgcgata ttgccaatca
     6181 aggtaatcct cgctggtcag ttcctgccct ggataagtgg tcgctgcaag acgaaggagg
     6241 ggcgccaggc tatccccgac tctccaacga gcgatctgat cggcattcat cgtcccgcaa
     6301 gttcgaacaa ttccaaatac tggtccacaa tccgttccca gctatagcgc tcctgagcca
     6361 gttgcaacat ttgcaggcgt gcagcctgtc gctgtccggc agattgttgt tccagttcaa
     6421 tcagcttcga cgacagttcc ttgtcgctcg agaagtaaat tgctgcgcca gccgttgtct
     6481 cccggttgaa cggattatca tggcaaagac agactgccgc agtcgccatc gcctccacca
     6541 gggccggatt cg
//
LOCUS       DAOTON010000036         6539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_10162_length_6539_cov_3.110580, whole genome shotgun sequence.
ACCESSION   DAOTON010000036 DAOTON010000000
VERSION     DAOTON010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10162_length_6539_cov_3.110580"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..374
                     /locus_tag="P5292_01320"
     CDS             3..374
                     /locus_tag="P5292_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRS37793.1"
                     /translation="MIVLESAFFAVLFLQSGLDKLLNWKDNLGFHQSHFATSPLARWG
                     RPMLLLLTMLELIAGGLSVVALLLQWTNPGSPIILCAFYLPALVFLCLFFGQRIAKDY
                     AGAAALVPYFILALFGILVAG"
     gene            complement(375..1454)
                     /locus_tag="P5292_01325"
     CDS             complement(375..1454)
                     /locus_tag="P5292_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRS37794.1"
                     /translation="MSGTAGGIHFDGKSAVPRTARLVIQEDHDVLVLHDEGGTARFIP
                     LSDLEVENTSGVFRLRFGENPEESLEIRDESFRAELLQTLRRKGHQGIYSRLVHGGAG
                     LHLLIALLLIGGIAFAYWFVVPWVAERAVSLLPERYDSAMGSTFYDSFVVDEEIDSVK
                     TQALRSFAAQLKLPASERQPKFTVVVSKEENAFALPDGNIVVYSGILKLLDRPEQLVA
                     LIGHENAHVTQRHSMKLLSRSLAGYLFISVALSDVNGVMAVLADNANALRNLSYSRAY
                     ESEADRIGLETLMANDIAASGMRELFEKLEAHHDLSIPGFLSTHPVGNERIAEVRAWE
                     TEHPYTASGKNAGLDSLFNVLKQGR"
     gene            complement(1454..2443)
                     /locus_tag="P5292_01330"
     CDS             complement(1454..2443)
                     /locus_tag="P5292_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HRS37795.1"
                     /translation="MENVTNTDAGSHLAPPPTRNYRLSFSGNGGEYFGITIVNWLLTV
                     VTLGLYYPWAKARELQYLYGATEFEENRFTFHGTGKEMFKGFIKALIIFGILMAAFIG
                     CAFTNHPLLGLVILYGGLFALIPLAIHGSYRYRMSRTSWRGIRFGYRGDRNELFVNFF
                     KWIFFTVITFGIYGAWMSMNLRNYVLNNVRFGNMKFRYKGNGGDYFVLNLKGYFLTIL
                     TLGIYIAWWQRDLFNYYVNNLSLHDEEREVKFRSTITGGGIFGMAIVNILILIFTLGL
                     GYAWVVTRSLQYVFRNIQPEGTIDFNRLEQTEAQYTDATGEDISDLLDFGFVI"
     gene            complement(2519..2851)
                     /locus_tag="P5292_01335"
     CDS             complement(2519..2851)
                     /locus_tag="P5292_01335"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HRS37796.1"
                     /translation="MSEHNHHREEVFSKSVRAGKRTYFFDVKSTRSNEYYITITESKK
                     RIGDDGNPFFEKHKIFLYREDFDKFMDGMREAMNHVVTVQPEPPVHHHNQSGNSSYSD
                     VDFDDLGK"
     gene            complement(2930..5854)
                     /gene="uvrA"
                     /locus_tag="P5292_01340"
     CDS             complement(2930..5854)
                     /gene="uvrA"
                     /locus_tag="P5292_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRS37797.1"
                     /translation="MSNVDKLDPRDFIVIKGARVHNLKNLDVAIPRNQFVVVTGLSGS
                     GKSSLAFDTLYAEGQRRYVESLSSYARQFIGRMDKPDVDYIKGISPAVAIEQKVNTRN
                     PRSTVGTSTEIYDYLKLLFARIGHTYSPVSGQEVKRHSVDDVVDFLIAFPQGTRAQLF
                     APLLLHPGRTLEEECNVLLQKGYSRLQVNQDIHRIEELLQPDQALFLRQLSESLGIQT
                     AQVKASGRKKKQELPAETGGPRFAPHLWVLIDRFALESDNDANRSRIADSIQTAFYEG
                     HGSCKVIVQPENGATSEHDFSNRFELDGLEFEEPSTALFSFNNPYGACKKCEGFGSVI
                     GIDEDLVIPDKSLSVYEGAIVCWRGEKMREWNDQLVKTAYKFDFPIHRPVFELTTEER
                     ALLWNGNKYFKGIREFFEWVETQTYKIQYRVMLSRYRGRTTCPECRGTRLRKDATYVK
                     ITGRSITDIVLMPVSESLRFFRELQLNAYEQEVARRILLEITNRLQFLSDVGLGYLTL
                     NRLSNTLSGGESQRINLATSLGSSLVGSMYILDEPSIGLHPRDTQRLIQVLLSLRDVG
                     NTLVVVEHDEEIMRSADHLIDIGPDAGRLGGELVYAGTVEKIASETRSYTGKYLSGQE
                     RIPLPYRRRKATSYIKLEGARENNLQNITVKFPLGLMTVVTGVSGSGKTSLVRKVLYP
                     ALKKIHGGFGDESGLYDKISGDYNKLSAVELVDQNPIGKSSRSNPVTYVKAYDEIRAL
                     YADLPLSRARKYKPAHFSFNVEGGRCEVCEGEGQVTIEMQFMADIHLVCENCGGKRFK
                     QEILDVSYKGKNVAELLDMTVDEAIEFFSSPIKSFGNDGKIATKLKPLQDVGLGYVKL
                     GQSSSTLSGGEAQRIKLASFLGKGNSHDHTLFIFDEPTTGLHFHDIRKLLLAFDMLLE
                     QGNSLIIIEHNPDVIKCADWVIDMGPEGGVNGGQVVFEGTPDELAKAEGNHTGHFLRD
                     KL"
     gene            6137..>6539
                     /locus_tag="P5292_01345"
     CDS             6137..>6539
                     /locus_tag="P5292_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRS37798.1"
                     /translation="MVSFQRMEDKDLVRLYQEGNQQAINELINRYKQRVYSAIFFLVR
                     DRDLADDLFQEAFIKIIQSLRSKHYSEQGKFLPWALRIAHNLVIDHFRKEKLMPMQRD
                     TDDFSVFDIIPANNRNAADQIIHDEKLAFVRS"
BASE COUNT         1453 a         1753 c         1747 g         1586 t
ORIGIN      
        1 ggatcatcgt attggaatcg gccttttttg ccgtactctt tcttcaatca ggattggata
       61 agctgctgaa ttggaaggat aatctcgggt ttcatcaaag tcatttcgcg acttcgcctt
      121 tggcccgttg gggccgaccg atgttactgc tgttaacgat gctcgagttg atcgccggcg
      181 gtttgtctgt tgttgccttg ctgttgcaat ggacgaatcc cgggagtcca atcattctct
      241 gtgcgtttta tttaccggcc ctggtatttc tatgtttgtt tttcggtcaa cggatcgcga
      301 aagactatgc aggcgcggcg gccctggtgc cgtatttcat cctggcactt ttcggaatac
      361 tcgtagccgg ttgatcagcg tccctgtttc agtacgttga acagcgagtc aagtccggca
      421 ttttttcctg atgccgtata aggatgttcc gtttcccatg ccctcacctc cgcgatgcgt
      481 tcgttgccta cagggtgggt gctcaggaag ccggggatgg agagatcgtg atgcgcttcc
      541 agtttttcaa agagctcccg cattccgctc gccgctatgt cattcgccat taaagtttcc
      601 agcccgatcc ggtcggcttc gctttcgtaa gccctggagt aggatagatt gcgcaaggca
      661 ttcgcattgt cggccaatac ggccatcacg ccattgacat cgctcaaggc aaccgagatg
      721 aacaggtagc ctgcaagact ccggctgagg agtttcatcg agtgtcgttg cgtgacgtgc
      781 gcgttctcgt gaccgatgag ggcgaccaat tgttccgggc gatccagtag tttcaggatg
      841 ccgctgtaaa cgacaatgtt tccatcgggc agggcgaagg cgttttcttc cttggaaaca
      901 acgacggtaa acttgggttg tcgttcgctc gctggtagct tcaattgagc cgcgaaagat
      961 cgcagggcct gggtcttcac cgagtcgatt tcttcatcga cgacgaagct gtcataaaag
     1021 gtgctgccca tggccgagtc gtagcgctcc ggcaacaggc tgaccgctcg ctccgccacc
     1081 cagggtacca caaaccaata ggcgaaggcg atgccaccga tcaacagcag ggcgatcaac
     1141 agatgcaagc cggcgccgcc gtgtacgagt cgggaataga ttccctggtg ccccttgcga
     1201 cgaagcgttt gaagtagttc ggcccgaaac gattcatccc ggatctccag cgattcttcc
     1261 gggttttcgc caaagcgtaa gcgaaatact ccggatgtgt tttcaacttc cagatcagac
     1321 agtggaataa agcgagcggt tccgccttca tcgtgcagta cgagcacgtc atgatcctcc
     1381 tggatcacca accgggcggt cctcggaacg gcgctctttc cgtcgaagtg aattcctccc
     1441 gcagtacccg acatcagatc acgaaaccga agtcgagcag gtcggaaata tcctccccgg
     1501 tcgcgtcggt gtattgggct tcggtttgtt ccagccggtt gaagtcgatc gtgccttccg
     1561 gctggatgtt ccgaaacaca tattgaaggc tacgggtcac cacccaggca tagcccagtc
     1621 cgagcgtgaa gatcaggatc aggatgttga cgatggccat tccgaaaata cccccgccgg
     1681 taatcgtcga acggaactta acctcccgtt cctcatcgtg caggctcagg ttgttgacat
     1741 aatagttgaa caagtcacgc tgccaccagg caatgtagat gcccaaggtc agaatggtca
     1801 ggaagtagcc cttcaggttc aggacaaagt agtcgcctcc gtttcccttg tagcggaatt
     1861 tcatgttgcc gaaacgcaca ttgttcagga cgtaattgcg caggttcatg ctcatccagg
     1921 cgccgtagat accgaaggtg ataacggtga agaagatcca cttgaagaaa ttcacgaaca
     1981 attcgttccg gtcgccccgg tagccgaagc gaataccccg ccaggaagtc cgggacatgc
     2041 gataacggta ggagccgtgt atggccagcg gaatcagtgc gaacagtccg ccatataaga
     2101 tcaccaatcc cagcagcggg tggttcgtaa acgcgcatcc gatgaaggcg gccatcaata
     2161 tgccaaagat gatcagggct ttgatgaacc ctttgaacat ttccttgccg gtaccgtgga
     2221 aggtgaagcg gttctcttcg aattccgtcg cgccgtacag gtactggagt tcacgcgcct
     2281 ttgcccaggg atagtaaaga ccgagcgtga cgaccgtcaa cagccagttg acgatcgtga
     2341 taccgaaata ttcccctccg ttaccggaga atgacagccg atagttgcgg gtagggggtg
     2401 gcgccaggtg agaaccggca tcggtgttgg tgacgttttc catggtgaaa gtgtggagta
     2461 gggaatagcg gaaatataaa agccccctga tcgggggctt gagaaggggt tatccggatc
     2521 acttaccgag atcgtcgaaa tcgacatccg agtaagaact gttaccggat tgattgtgat
     2581 gatgcaccgg aggctcgggt tgaacggtca cgacgtgatt catcgcttcc cgcataccgt
     2641 ccatgaactt gtcaaaatcc tcccggtaga gaaagatctt gtgcttctcg aagaacggat
     2701 tgccatcgtc accgatccgc ttcttgctct cggtgatggt gatgtaatac tcattggatc
     2761 gcgtgctctt tacgtcaaaa aagtaggtcc gttttccggc acgcacggat ttggaaaaca
     2821 cttcttcgcg gtgatgatta tgctccgaca tacgggcagt tgtgtaaggg tttgagcaaa
     2881 aatagaaaat aaatccaatg cgcaaggacg actttccggc cctttaacct catagtttgt
     2941 cgcgcaggaa gtgccctgta tggttaccct ccgccttggc cagctcgtcc ggagtacctt
     3001 caaagacgac ctgcccgcca ttgaccccgc cttccggtcc catgtcgatc acccagtcgg
     3061 cacatttgat cacatccggg ttatgttcaa tgatgatcag gctgttacct tgttccagaa
     3121 gcatgtcgaa ggccagcagc agcttacgga tatcgtggaa atgcaagccc gtagtgggtt
     3181 cgtcaaagat gaagagggta tggtcgtggg agttgccttt gcccagaaaa gaggccagtt
     3241 tgatccgctg ggcttcccca ccgctcaggg tgctcgacga ttgtccaagt ttcacatagc
     3301 ccaacccgac atcctgcaac ggcttcagtt tcgtcgcgat ctttccgtcg tttccaaagc
     3361 tcttgatcgg tgaactgaag aactcgatgg cttcgtcgac ggtcatatcg agcagttcgg
     3421 caacattctt tcccttatag ctgacatcca ggatctcttg cttgaatcgc ttaccgccac
     3481 agttctcgca aaccaggtgg atgtcggcca tgaactgcat ctcgatcgtc acctgtcctt
     3541 cgccttcgca cacttcgcac cgaccgcctt ccacgttgaa cgagaaatgc gccggcttgt
     3601 acttccttgc tcgcgaaagc gggaggtcgg catacagagc gcggatttca tcgtacgctt
     3661 tgacatacgt gaccggattg gatcgcgagg attttccgat cggattctgg tccaccagct
     3721 cgacggcgga aagcttgttg tagtcgccac tgatcttgtc gtacaagcca ctctcatcac
     3781 caaagcctcc atggatcttc ttgagagcgg ggtatagcac tttccgtaca agggatgtct
     3841 ttccgctacc gctcacaccg gtcaccacgg tcatgagccc caacgggaat ttcacggtaa
     3901 tgttttggag gttgttttcc cgggcgcctt ccaatttgat atagctggtg gccttccgtc
     3961 gacggtacgg taaggggata cgttcctgac cgctcaggta cttaccggta taactgcggg
     4021 tctccgacgc gatcttttcg accgttcctg catataccag ttctcctccg agtcgtcccg
     4081 catccgggcc gatgtcgatc aggtgatcgg ccgatcgcat gatctcttcg tcgtgctcaa
     4141 ccaccaccaa tgtattcccg acgtcccgaa ggctaagcaa gacctggatc aggcgctggg
     4201 tgtcacgcgg gtgcagtccg atgctgggtt cgtccaggat gtacatgctg cctaccaggc
     4261 tgctgcccaa agaagttgcg aggttgatgc gctgcgactc accgccggaa agggtattcg
     4321 acaggcggtt gagcgtcagg tagccgagtc cgacatcact gaggaattgg agacggttcg
     4381 tgatctccag caggatgcgt cgggcgactt cctgctcgta ggcgttcagt tgcagttccc
     4441 ggaaaaaccg gagactttca ctcaccggca tcagcacgat gtccgtgatc gatcggccgg
     4501 tgatcttgac ataggttgcg tctttccgaa ggcgagttcc gcggcattcc ggacaggtgg
     4561 tacggccgcg gtaacggctc agcatcaccc ggtactggat cttgtatgtt tgtgtctcca
     4621 cccattcgaa aaactcccgt atccctttga aatacttgtt tccgttccac aaaagcgcgc
     4681 gttcttccgt tgtcagctcg aaaaccggcc ggtggatcgg gaaatcaaac ttataggcgg
     4741 tcttgaccag ttgatcattc cactcgcgca tcttctcgcc gcgccagcag acaatggctc
     4801 cttcgtacac cgacaaactt ttatcgggaa tgaccaggtc ctcgtcgatg ccgatcacgg
     4861 aaccgaatcc ttcgcacttc ttacatgcgc cgtaaggatt gttgaaagag aagagcgccg
     4921 tgctgggttc ttcgaattca aggccgtcga gctcgaagcg attcgagaaa tcatgctcgc
     4981 tggttgcccc attttccggc tgtacgatca ctttgcaact gccgtgtccc tcgtaaaacg
     5041 ccgtctgtat agaatcggcg atacgcgaac ggttggcatc gttgtcggat tcaagtgcaa
     5101 agcggtcgat caggacccac aaatggggtg caaagcgcgg accaccggtc tcagcgggca
     5161 attcctgttt cttctttcgt ccgcttgctt tgacctgagc agtctgaatg cccagcgatt
     5221 cggaaagctg acgcaggaag agcgcctgat ccggttgcag caattcttcg atccggtgaa
     5281 tgtcctggtt gacctgcaaa cggctgtagc ctttttgcaa cagtacgtta cattcttctt
     5341 ccagtgttcg gccgggatgg agcaggagcg gagcgaacag ctgcgcccgg gtaccttgcg
     5401 ggaaggcgat caggaaatcc acgacatcat ccacactatg tcgcttgact tcttgtccgc
     5461 tgaccggtga ataagtgtgt ccgatccggg caaagagcaa tttcagatag tcgtagatct
     5521 ccgtggaagt cccgacggtc gaacgcggat tgcgggtgtt gaccttttgc tcaatggcta
     5581 ccgccggaga aatgcctttg atgtaatcca catccggctt gtccatccgt ccgatgaact
     5641 gacgtgcata agaactcagg ctttcgacgt agcgacgctg cccttcggca tacagcgtgt
     5701 cgaacgcaag ggaagatttc ccggatcctg aaagcccggt gaccaccacg aattggttgc
     5761 gtggaatcgc tacatccagg tttttcaggt tgtggacgcg tgcacctttt attacgataa
     5821 aatcgcgggg atccagttta tcaacgttcg acataaagcg tcttggcaat ctgcgaaggt
     5881 actgaaaaag ggcctttttt agcggatcgc cctgccttgt gtaagggaaa ggttggaaga
     5941 atgtaagatt cctgttgaaa aaagccggaa taatcgaagg ccgcatttgc ttttctcaaa
     6001 accattcgct acatttgatt caacgtaacc atccggctac gttttctttt acccacaacg
     6061 ccctataaaa ggaggaatca tagcgctaca tctactctaa taagacgact caccgttcta
     6121 ttaagcagaa gcgactatgg tatcattcca acggatggag gataaagacc ttgtgcgtct
     6181 ttatcaagag ggcaatcagc aagccattaa tgaactgatc aaccgctaca aacagcgggt
     6241 gtattcggct attttctttc tggtccgcga ccgggatctg gccgacgatc tctttcagga
     6301 ggctttcatc aagatcattc agagtttgcg cagcaagcat tacagcgaac aggggaagtt
     6361 cctgccctgg gccttgcgca tcgcgcacaa cctggtgatc gatcacttcc gcaaggaaaa
     6421 gctgatgccc atgcagcggg atacggacga tttcagcgtg ttcgatatca tcccggccaa
     6481 taaccgcaac gcggccgacc agatcattca cgacgaaaag ctggcctttg tccgcagtt
//
LOCUS       DAOTON010000037         6527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_10188_length_6527_cov_3.378399, whole genome shotgun sequence.
ACCESSION   DAOTON010000037 DAOTON010000000
VERSION     DAOTON010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10188_length_6527_cov_3.378399"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="P5292_01350"
     CDS             <1..1045
                     /locus_tag="P5292_01350"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRS37799.1"
                     /translation="SGLQENFAEALGLFEHLLANCKPDETALKNMIEDEIKARENNKL
                     EISYISGSAMNAYGKYEGKNPFNDVLSSEQLRALKGGELTEILHGLTGYAHNILYYGP
                     LAVTEATAVVARHHTDGKAPKTVPPAKQYRYRTVTDNQVYFTQYDQMVKAQINWTRNV
                     MPFDVAVLPKVRLFNQYFGGDMSSIVFQEIRESKALAYSSYSFFAAPGRKEDPYSLFA
                     YIGTQADKITQAIPAMNQLLTELPNSEKSFTQAKQAIRNNIAASRITKSQILFDYLTA
                     QRRGIEFDIRRPVYEQVENLKFEDIQTFYNEHISKKPFVITVLGDKSKIDLTSLGKFG
                     AVKELSMEELFGY"
     gene            complement(1112..1999)
                     /locus_tag="P5292_01355"
     CDS             complement(1112..1999)
                     /locus_tag="P5292_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRS37800.1"
                     /translation="MNAPTHTRRDTYFLLAILAIIWGSSYILMKRGLLAYSPQQIGAL
                     RIFTAGLVMLPFAWRSRAKVQRHHWQFLILTGLLGNAIPSILFPLSETRISSALAGMI
                     NTLTPLFTLLIGFLMFGMQATRYRMLGLLIGLAGAVLLILGRGWGEASADARYSMFVV
                     LATVCYAFSVNILRHKLHDLDPISTTSYSLLLMGTPMGIYLFTTDFVTRTSEHPKAFV
                     SMGSILLLGMLSTAFSTVLFNRLIKQSGALVASSVTYLAPAVAALWGLLDNEEIGLLH
                     LAGLGLILSGVYLIGRGGK"
     gene            complement(1996..3267)
                     /locus_tag="P5292_01360"
     CDS             complement(1996..3267)
                     /locus_tag="P5292_01360"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRS37801.1"
                     /translation="MTAFSDFKLNKQLLTAIADQGWDAPTPIQERAIPQILAGHDILG
                     IAQTGTGKTAAYVLPILMKVKYAQGEDPRALILVPTRELVIQVADWVKALGKNTDLRT
                     VALYGGVGIKPQAAALEAGCDIIVATPGRLMDLYLPGHVSLKKITTMVLDEAERLLDM
                     GFRPQIGRILEVVPRKRQNMLFSATFGDRVKRISEEFLVAPMEIRIEERIRPARSVTQ
                     SVYFTPNLKTKINLLGELLATDGFDRVILFCKTKETATNIARYLSRKYGEETVRVIHG
                     NKAQNTRLNAVEQFRRHEVRFLVSTDVAARGIDIPSVTHVFNFDVPLVYEDYVHRVGR
                     TGRAFETGAAITFCAPDDEYHLRKIQKLIGEKIPVVSLPERVEVPETPFEEQQRILRE
                     IDMQRRKEDPTYQGAFHERKRKSDKTKKRRS"
     gene            complement(3374..5419)
                     /locus_tag="P5292_01365"
     CDS             complement(3374..5419)
                     /locus_tag="P5292_01365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HRS37802.1"
                     /translation="MKRTLLLVSLAAAAAALATTACKQSEAKKGVGIDLANIDSSAAP
                     GNDFFQFANGSWIKANPIPNDQSRWGTFNILGDNNRKNLRGLAEEAAAKTGAAKGSPE
                     QMVGDFWFTAMDTVSIEKAGAEPIKGDMSTIDGLKDMKSVLEYVSRLQKWGANPMFTF
                     YAYQDPKNSEVVVPQLYQGGLSLPDRDYYVKTDARSQEVRKEFIAHVVKMFELYGLDN
                     ATATKYANTVMRIETALANASMTRVEQRDPFKTYNKVSIDDLNKLTPSIDWATMMDQL
                     EVPKKYDYLVLGQPDFLKELEKQLKSSSLDDWKIYLKWNVLNNAGNILSDAFVMEDFR
                     FNNQFMNGQKEIQPRWKRMVAMTDGMVGDALGQMYVAKYFPPEAKKKADELVSNLMAV
                     YNDRIQKLDWMSDETKKKALEKLSTIQRKIGYPDKWKDYAGLSIDRSSFYQNILNAAK
                     WNYEYMVKKVGQPVDRAEWGMTPPTVNAYYNPSLNEIVFPAGILQPPFFNAQADDAVN
                     YGGIGAVIGHEITHGFDDEGRNYDAHGNLNSWWTSEDSAKFTAKAQRIVEQFNGYVVL
                     DSLHVNGELTLGENIADLGGISISYEAFKRTPQGKGNEKIDGLTPDQRFFMGFATIWA
                     GSIRPEMAAQRLITDPHSPAKYRVNGPLSNLQEFYSAFGLKEGDALYRPDSTRAKIW"
     gene            5583..6167
                     /gene="lptC"
                     /locus_tag="P5292_01370"
     CDS             5583..6167
                     /gene="lptC"
                     /locus_tag="P5292_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRS37803.1"
                     /translation="MVKDVKTENRRSPLWLLILSMAVLTSCENDLEKVKLYGKAKELP
                     VETGTNIKILYSDSARVQVEVNAPVLDRYDQENPYVEMPKGLRAFFYNDRMEVKSRLT
                     ADYGIRYERQQRMEARKNVVVVNEKGEQLNTEHLVWDEKQEKLLSDEFVKITTKDEII
                     FGNGFEANQDFSKYRIFKIKGTISINNPRNASHP"
     gene            6151..6369
                     /locus_tag="P5292_01375"
     CDS             6151..6369
                     /locus_tag="P5292_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37804.1"
                     /translation="MLRILELAWLGIALASAGIAVYQFINDGSESAIWMLFVMVVSGI
                     MYTIRHRQRVRYDQGRQQPQQDEESRYH"
BASE COUNT         1488 a         1812 c         1753 g         1474 t
ORIGIN      
        1 gtccggcctg caggagaact tcgctgaagc gctcggactc ttcgaacacc tgcttgccaa
       61 ttgcaaaccg gatgagacgg ccttgaagaa catgatcgag gacgagatca aggctcgcga
      121 aaacaacaaa cttgagatct cttacatcag cggttccgcc atgaacgcct atggcaagta
      181 cgaaggaaag aatccgttca acgatgtact cagttcggaa caattacgtg ccctcaaagg
      241 cggcgaactc acggagatcc tgcatggtct taccggctat gcacacaaca tcctttacta
      301 tggaccgctc gcggtaacgg aagcgacggc cgttgtggct cgtcatcata ccgacggaaa
      361 ggccccgaaa accgttccgc cagcgaagca ataccgctac cgtacggtga ccgacaatca
      421 ggtgtatttc acccagtacg accagatggt gaaagcccag atcaactgga cacggaatgt
      481 catgccgttc gacgttgcgg tgcttccgaa agttcgtttg ttcaaccagt atttcggtgg
      541 cgacatgtct tcgattgtgt tccaggagat ccgcgaaagc aaggccctgg cctactccag
      601 ctattcgttc ttcgccgcgc ccggtcgcaa ggaagatccg tacagcctgt ttgcctacat
      661 cggcacacag gccgacaaga tcacccaggc gatcccggcc atgaatcagt tgctgaccga
      721 actgccaaac tcggaaaaat cgtttacgca ggccaaacag gccattcgca acaacatcgc
      781 cgcttcccgg atcaccaagt cgcagatcct cttcgattac ctcactgcac agcgtcgcgg
      841 gatcgaattc gacattcgtc gtccggttta cgaacaagtc gaaaacctga agttcgaaga
      901 cattcaaacc ttctacaacg aacacatttc caaaaagccg ttcgtgatca cggttctcgg
      961 cgataagtcc aaaatcgacc tcacctcttt gggaaaattc ggggcggtga aagaactttc
     1021 aatggaagaa ttgttcggct attgatccgg acaagaatcc aacaagaaag ccgcgctgaa
     1081 caacagcgcg gctttcttgt ttaaggcgat ctcacttccc tcccctgccg atcaggtaaa
     1141 cgcctgaaag gatcagtccg agtcccgcca ggtgcagcag tccgatctct tcgttatcca
     1201 gaagccccca cagcgcggca acagccggcg ccaggtaggt gactgaactt gccaccagcg
     1261 cgcccgactg cttgatgagg cggttgaaca gcacggtaga aaaggcggtc gagagcatgc
     1321 ccaacagtaa gatgctgccc atactgacaa aagccttcgg gtgttccgaa gtccgggtga
     1381 caaaatcggt agtgaagaga tagatcccca tcggtgtccc catgaggagc aaagagtaag
     1441 aagtcgttga gatgggatca agatcgtgga gcttatggcg gagaatgttc acgctgaacg
     1501 cgtagcaaac cgttgccagg accacgaaca tggagtatcg cgcatccgcg gaggcttcac
     1561 cccagccacg tcccaggatc aacaagaccg cgccggcaag accgatcagc agtccgagca
     1621 tgcggtagcg ggtagcttgc ataccgaaca tcagaaaacc gatcagcaag gtgaacaagg
     1681 gcgtcagggt attgatcatc ccggcaaggg cgctggagat tcgtgtttcg gataacggaa
     1741 aaaggatcga ggggatcgcg ttacccaaca ggccggtcag gatcaggaac tgccagtggt
     1801 ggcgctgcac tttcgcgcgc gatcgccacg caaacggcaa catcaccagg ccagccgtga
     1861 agatgcgcaa agcgccaatc tgctgtggag agtacgccag caaaccgcgc ttcatcagga
     1921 tatacgaact gccccagatg atcgccagaa tggccaataa gaagtaggta tcgcgacgcg
     1981 tgtgtgtcgg ggcgttcatg aacgacgctt tttcgtttta tcggatttgc gtttccgttc
     2041 gtgaaaggct ccctgataag tgggatcctc cttccgtcgc tgcatgtcga tctcccgcaa
     2101 gatgcgttgt tgttcttcga aaggcgtttc cggcacctcc acccgctccg gcaacgacac
     2161 aacgggaatt ttctcaccga tcaatttctg gatcttacgc aagtggtact cgtcatcggg
     2221 cgcacagaag gtgattgccg cccccgtttc aaaggcacgc ccggtgcgtc cgacccggtg
     2281 cacataatct tcgtacacca ggggaacatc gaaattgaag acgtgcgtaa cggacggaat
     2341 gtcgataccg cgtgcggcga catcggtgct gaccaggaag cgtacctcat gacggcggaa
     2401 ttgttctacc gcgttcaggc gcgtattctg cgccttgttg ccgtggatca cccggacggt
     2461 ctcttcaccg tactttcttg acaagtagcg cgcgatgttg gtagcggtct ctttcgtctt
     2521 acaaaacagg atgacccggt caaagccgtc ggtggcaagc aattctccca gcaaattgat
     2581 cttggtcttc aggttgggtg tgaaatagac cgattgcgtg accgagcgtg ctggccggat
     2641 tcgctcctca atacggatct ccataggcgc caccaggaat tcttcggaga tccgtttgac
     2701 gcggtcaccg aaggtggcgg agaataacat gttctgtcgt tttcgcggca ctacttccag
     2761 gatacgaccg atctgtggcc gaaaacccat gtccagcagt cgctccgctt catcgaggac
     2821 catcgtcgtt atcttcttca acgaaacatg acccggcagg tagaggtcca tgaggcggcc
     2881 tggtgtcgcg acgatgatat cgcacccggc ttcaagcgct gccgcctgcg gtttgatacc
     2941 cacgccgccg tagagcgcta ccgtacgcag gtcggtgttc tttccaaggg ctttcaccca
     3001 atccgctacc tggatcacca actccctcgt cggaacgagg atcaaggcac gcggatcttc
     3061 cccttgcgcg tatttgacct tcatcaggat gggcagcaca tacgccgctg tcttacccgt
     3121 gccggtctgg gcaattccca aaatatcgtg gccggcaagg atctgcggaa ttgcacgctc
     3181 ctggatcggc gtgggcgcat cccatccctg gtcggcgatc gccgtcagca gttgcttgtt
     3241 gagtttgaaa tcggaaaagg cggtcacggg tggaattggc tgcaaaggac gcaaaagtaa
     3301 aggagaaatg cagtggacta aaaacaggaa agccccggaa tgtccggggc tttcagttta
     3361 tacgtacaat cgattaccag atcttggcac gggtagaatc cggacgatac agggcatctc
     3421 cttctttgag gccgaatgcg ctgtaaaact cctgcaggtt gctcaacggg ccgttcacac
     3481 ggtatttagc cggtgaatgc gggtccgtga tgaggcgctg tgccgccatc tcgggacgga
     3541 tgctgccggc ccagatcgtt gcaaagccca tgaagaagcg ctgatccggc gtgaggccgt
     3601 cgatcttctc gttgcctttt ccttgcggcg tacgcttgaa ggcttcatac gagatgctga
     3661 taccgccgag gtcggcgatg ttctcgccca gggtcagttc gccgttcaca tgcaggctgt
     3721 cgagtaccac gtagccgttg aactgttcta cgatgcgctg cgccttcgcc gtgaacttcg
     3781 cggaatcttc cgaggtccac cagctgttca ggttgccgtg cgcgtcgtag ttacgacctt
     3841 cgtcgtcgaa gccgtgggtg atctcgtgac cgatgaccgc gccgatgccg ccgtaattca
     3901 ccgcgtcatc cgcctgtgca ttgaagaacg gaggctgcag gatgccggcc gggaatacga
     3961 tctcgttcag ggaaggattg tagtaagcat tcacggtagg aggtgtcata ccccactcgg
     4021 cgcgatccac cggttggccg actttcttca ccatgtattc gtagttccat ttggcggcat
     4081 tgaggatgtt ctggtagaac gagctccggt cgatgctcag acccgcatag tctttccact
     4141 tgtccgggta gccgatcttg cgttgaatgg tgctgagttt ctccagcgcc ttcttcttcg
     4201 tttcgtcact catccagtcg agtttctgga tacggtcgtt gtacacggcc atcaggttcg
     4261 acaccagctc atcggccttc ttcttggctt ccggcgggaa atacttggca acatacattt
     4321 ggccgagtgc gtcgccgacc attccgtcgg tcatcgcgac catgcgtttc cagcgtggct
     4381 ggatctcttt ctggccgttc atgaactggt tgttgaagcg gaagtcttcc atcacgaacg
     4441 cgtcgctgag gatgttgccg gcgttattca gcacattcca cttgaggtag attttccagt
     4501 cgtccaacga acttgatttg agttgcttct cgagctcttt caaaaagtcg ggctgaccca
     4561 gcaccaggta atcgtacttc ttcggtactt ccaactggtc catcatcgta gcccagtcga
     4621 tgctcggcgt gagtttgttg aggtcgtcga tgctgacctt gttgtaggtc ttgaacggat
     4681 cgcgttgttc cacgcgggtc atcgacgcgt tggcaagcgc cgtctcgatg cgcatgaccg
     4741 tattggcgta tttcgttgcc gttgcgttgt caagtccgta gagttcgaac atcttcacca
     4801 cgtgagcgat gaactccttc cgtacttcct gggaacgcgc gtccgtcttc acgtagtagt
     4861 cgcgatccgg cagggagagg ccgccctggt ataactgcgg aacaacgact tcgctgttct
     4921 tgggatcctg gtaagcatag aacgtgaaca tcggattggc tccccacttc tgcagacggg
     4981 aaacgtattc caacacgctc ttcatgtcct tcagaccgtc gatcgtgctc atgtcgccct
     5041 tgatcggttc ggcaccggct ttctcgatgc tgacggtatc catggcggtg aaccagaaat
     5101 cgccgaccat ttgttcagga cttcctttcg cggcgccggt cttggctgct gcttcttcag
     5161 caaggccacg caggtttttc cggttgttgt cgcccaggat gttgaatgtg ccccaacggg
     5221 attgatcgtt cgggatcgga ttggctttga tccaactgcc attggcaaat tggaagaagt
     5281 cgttaccggg agctgcgctc gagtcgatgt tggcgagatc gatgccgaca cctttcttcg
     5341 cttccgactg tttgcaagcg gtcgtcgcaa gcgcagctgc ggcggcggcc agcgaaacca
     5401 ggaggagagt acgtttcatt acaggttgtt tggtttaagg cttgcaaaaa taaaagaatc
     5461 aatgcagggc acttgaccct taaccaacct taacacaaac caacattctc attctcaata
     5521 tgtaggatta ttcctgaaag aacaggccat ttgccgttca ccgcaattcg gcttaggttt
     5581 gcatggtgaa ggatgttaag acggaaaatc gccggagtcc actgtggctg ctcatcctga
     5641 gcatggccgt gctgacttcc tgtgagaacg accttgaaaa agtcaaactc tacggaaagg
     5701 cgaaggaact tccagtcgaa accggaacaa acatcaagat cctgtacagc gattccgccc
     5761 gcgtgcaggt agaagtcaat gcgccggtcc tcgaccgtta cgaccaggag aacccctatg
     5821 tcgagatgcc gaaaggattg cgtgcgttct tttataacga ccgcatggaa gtcaaaagcc
     5881 ggttgacagc cgattatggc attcgttacg agcggcagca gcgtatggaa gcgcgaaaaa
     5941 acgtggtcgt tgtgaatgaa aaaggcgaac aattgaatac cgaacacctc gtctgggacg
     6001 aaaaacagga aaagctgctg agcgacgaat tcgtcaaaat caccacaaaa gacgagatca
     6061 tcttcggaaa cggtttcgag gctaaccagg atttctcgaa ataccgtatc tttaagatca
     6121 aaggaaccat ctccatcaac aacccgcgca atgcttcgca tccttgaact cgcctggctt
     6181 ggcatcgcgc tggccagtgc cggtatagcg gtctatcaat tcatcaacga cggatccgaa
     6241 tccgccatct ggatgctttt cgtcatggtc gtttccggca tcatgtatac gatccgacac
     6301 cgccagcggg ttcgttacga ccagggccgt cagcaaccgc aacaggacga agaaagccgc
     6361 tatcactaat ccattcatgt accaacgcat ccgtgcctgg gcttaccgga acctcattac
     6421 ccgcttcctg ttccactttt tgcagaacat cgtcctgtgg ttcgtcgtgt tctacgcgct
     6481 ccacctgatc tggcccgacg aaaaagaaca ggtcttctcc cgcaccc
//
LOCUS       DAOTON010000038         6458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_10324_length_6458_cov_3.022802, whole genome shotgun sequence.
ACCESSION   DAOTON010000038 DAOTON010000000
VERSION     DAOTON010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10324_length_6458_cov_3.022802"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..2316)
                     /gene="recQ"
                     /locus_tag="P5292_01380"
     CDS             complement(103..2316)
                     /gene="recQ"
                     /locus_tag="P5292_01380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRS37805.1"
                     /translation="MSSTDNLSTALHEHFGFSHFRHEQEAIISRVMANKDVLAIMPTG
                     GGKSICYQLPALLKPGLTVVFSPLIALMKDQVDSLRLNGIHAAFLNSSQSAEEQQAVI
                     RSVDAGEIRILYIAPERLASRRSEHIEWLRRVGVALFAIDEAHCISHWGHDFRPDYLG
                     LSVLKVNFPDTPIIALTATADRQTRLDILEKLGLHNAEVFVSSFNRENIRYTVDDKDD
                     FEYKLREFLDARKGQSGVIYCLSRNKTEELALRLEDLGYSAAAYHAGLDNKTRQERQD
                     QFKKDDLKIIVATIAFGMGIDKSNVRFVVHANLPKNIESYYQETGRAGRDGLASEALL
                     FYNPGDLMILKQFAAVDNNPAQSRIMLRKLEQMADYCTSKKCRREILLRYFDEDYTGP
                     CNNCDRCLSTEVEETFDGTRIAQIALSAVVRLKEKFGIGYVIDFLKGSNSSKLWEEHR
                     YLPTFGKGAEHTRDEWRSYLNDLIRQGFLKQSEGEFTLLRLTPEAHKVLYENHPVQLV
                     RVHSRKSNRELRQRNATRPNNPELPHPELNQLLRQLRRQIADSEGVAAYMVFSDASLA
                     ELVTYLPQTTEALHSISGFGQAKVDHYGSTIVEVIRAYCGSNGIEHAPERKARKKSKS
                     SGPRLDKDHLNTRERTLIQFQEGKSIQEIATERKLSPLTIEGHLIEFIKSGEINVLKF
                     ITKERLQELLQHFESNESLINGTLTQAKESLQNQYTFNEIKAAMAYREKKVTERR"
     gene            complement(2445..>6458)
                     /locus_tag="P5292_01385"
     CDS             complement(2445..>6458)
                     /locus_tag="P5292_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HRS37806.1"
                     /translation="ALLLLTLTVNVSNNNAPVLAYVGNVPFAASAVYPASGEPAERFR
                     FEIRYTDLDGDLPQPGYPRLLLDFEGNGLFTDANDRSISLLQSNPLDVDVTDGKNYYC
                     ETSGLPESYYYQHTFIVEDPTGCTTSYGPNAGPVVTFAPDLSIFANDITFSDPNPAPG
                     SPLTITARIHNYPGSANASQLTIRLKNQFDTTIVYPDQFIYTPINPYPYVDVVWNITT
                     PAVPAWCPMQVIVDATNALSEPNELDNQAIRPFTNGVYELPGDIMITASANPEVASVC
                     GSVTVCGTAYYRNTAIPLTDSSCAGATVTCRINETGQSASGYTNSSGEYCITLPTAAT
                     PGPYTVNVSITDFTLTGDTITDFELTGSCPTNNENDPGGGAGGGGEIEPSCNIIRDLL
                     HQGSGIITLHATLGITDVVVAGDSLHVYFSVENGGCDSITRQSIQQIQIPTALPSGIM
                     NTVPPLAPGGSYAIDLGYIRFPNPGQTYIRSICDATDSVTESNEFNNILIKNITVLPA
                     LPDLLPTAMNLRGQSSLLKCQLSTLIYSIQNTGGVPSGTYQARLDIYRNDTLESTQTQ
                     TFPSINGISSSNITFTPTLAGTGTYRFELNCDPSNFITELSENNNNAVDQFVLDDCKA
                     DLQMLSCGSVIITPTDPTPGGNMVISASIYNGGQLAANGPFTVDFDVNGTHYYGTYNG
                     NIQPDSSKTVSVLATVPPYGSDTLTVIADNSGVVPELDENNQVKQPLCYNFSLSNYSH
                     CPPSQNALINSSYPVNAPVTLATNVFNAGLYQAGNATIRFEVSGPGISGWALAGNISK
                     YLPGTCGCPIYVQLPLPYSFPQTGSYTIRITIDPANQYPECNEADNVLLIPVNISNSP
                     DYRVLSQYIAPDILNPEPGQQVTLDLTYENIGAYRPDSLELLVRGDTSDLDSILVAPL
                     TNGTFNTVRLAQTWSSQIRGVHVIRSIVDHDDVLSESDETNNEATRAIVVGRSPNLRF
                     TFFATTNTNPSPGAQININAIVQNNGYTRCTGLFGLYYLDNSNFEVLIATRNLNLDSN
                     ESANFSIPWFVAEARTTIIGRLLNCTPIEFNSTDNVAQLDIGRLTLNAFALPASCDSN
                     GTGLAIAAISGGQPPYFIQWSTGANVDSIRAVNGTYYVSVLDAEGTALSDSANIACLA
                     FVNGLTIKSWLSGFYDPGADNMRATIDPINMDTIADSIIVVLADTSTPFASLYQGTSL
                     LGTNGWSYVHFPQNVAQRSYYIVLRNRNHLETWSAAPVPFLESGTAYDFTDASGKAYG
                     ANQMELEPGVWGIWSGDVNQDRLIEATDYSLAENAVVNFAFGYVPEDLTGDQLVEASD
                     YSLIENNLLLFLFVITP"
BASE COUNT         1422 a         1546 c         1837 g         1653 t
ORIGIN      
        1 ccggaaataa cttttaacca accctgtctg tcaggtgaag ttctaactat tacaaattag
       61 ttactaatta ctagttacta attacgaatc tccaatcccc aatcacctcc gctccgtcac
      121 cttcttttcc ctgtaagcca tcgctgcttt gatttcattg aaggtgtatt ggttttgcag
      181 ggattccttc gcttgcgtca gcgttccgtt gatcagagat tcattggatt cgaagtgttg
      241 taacaattct tgaagtcttt ctttggtgat gaacttgagt acgttgatct ctccggattt
      301 gatgaactcg atgagatgac cttcgatcgt caacggcgat agctttcgtt cggtcgcgat
      361 ctcctggatg gacttacctt cctggaattg gatcagggtg cgctcgcggg tattgagatg
      421 gtctttgtcg agccggggac cggaggactt tgattttttc cgagcctttc gttccggtgc
      481 gtgctcgatg ccgtttgacc cgcagtacgc gcgtatgact tctacaatcg tggacccgta
      541 atgatcaact ttcgcctgcc cgaagcctga aatcgagtgt agcgcttccg tggtttgcgg
      601 caggtaggtg accaattctg ccagggaggc gtccgaaaag accatgtagg ccgcaacacc
      661 ttccgaatcg gcgatctgtc gtcggagttg gcgaagcaat tggttcaatt caggatgagg
      721 cagttctgga ttgttgggcc gggtagcgtt gcgctgccgg agttctcgat tggatttgcg
      781 actgtgtact ctgacgagtt gaaccggatg gttttcgtac aggactttgt gggcttccgg
      841 agtaagccgg agcagggtga attcgccctc tgattgtttc aggaatccct ggcggatcaa
      901 atcgttcagg tacgatcgcc attcatcgcg ggtatgttct gctcctttcc caaaggtggg
      961 caggtagcga tgttcttccc atagtttgga cgaattggac ccttttaaaa aatcgattac
     1021 atagccaatc ccgaatttct ccttcagccg tacaaccgca gacaaggcaa tttgtgcgat
     1081 ccttgttccg tcgaaagttt cttccacttc ggtggaaagg caacgatcgc agttgttgca
     1141 ggggccggta tagtcttcgt cgaagtagcg gagcaggatc tcccgcctgc atttttttga
     1201 cgtgcaataa tccgccatct gttccaactt ccgaagcatg atcctcgatt gcgccggatt
     1261 gttgtcgacc gccgcgaatt gtttcagaat catcagatca cccggattgt aaaacaacag
     1321 ggcttcgctc gccaggccgt cgcgaccggc tcttccggtt tcctgataat aactttcgat
     1381 gttcttgggc aagttggcgt gtacgacaaa ccggacgttc gatttatcga tccccatgcc
     1441 gaaggcgatg gtcgcgacga tgatcttcaa atcatccttc ttgaactggt cctgtcgttc
     1501 ctgtcgcgtc ttattgtcca agcccgcatg atatgcggcc gcactatacc cgaggtcctc
     1561 caggcgtagt gcgagttctt cggttttgtt tcgtgataaa cagtagatca caccactttg
     1621 cccttttcgg gcatcgagga attcgcgcag tttgtattca aagtcatctt tgtcatccac
     1681 cgtatagcgg atattctccc ggttaaaact gctgacgaaa acctctgcgt tgtgcaaacc
     1741 aagtttttcg aggatgtcca gacgggtttg acgatcagca gtcgcggtga gggcgatgat
     1801 cggtgtgtcg ggaaaattga ccttcaggac ggaaagtccc aaataatcgg gacggaagtc
     1861 gtggccccaa tgggaaatgc aatgcgcttc atcgatcgcg aagagggcaa cgccgaccct
     1921 gcgaagccat tcgatgtgtt cgctgcgtcg cgaagcaagg cgttcgggag ctatgtagag
     1981 gattcgtatt tctcccgcgt cgacactcct gatcaccgcc tgctgttctt cggcgctttg
     2041 cgatgaattg agaaaagcgg cgtggattcc gttcaggcga agactgtcga cctggtcctt
     2101 catgagcgcg atcagcgggg aaaaaaccac cgtcagaccg ggtttcaata gcgcgggtaa
     2161 ctgataacag atcgacttgc cgccgccagt cggcatgatc gccagcacat ccttgttcgc
     2221 catcacccgg ctgatgatcg cttcctgttc atgccggaaa tgcgaaaagc cgaagtgttc
     2281 gtgtagggca gtggagagat tgtctgttga actcatgcga aagcaaaggt aagcccatct
     2341 tattctgcgg gaaagtccat ggaaaaatat tcgcttttat tagggcctta aaagagaaaa
     2401 ggctgcacaa caaggcagcc ttttcggatt agtgctaaga tttctcaggg tgttatcaca
     2461 aacaggaata ataacaggtt gttttcaatc aggctatagt cggaagcttc cacaagttgg
     2521 tcgccggtaa gatcttccgg cacatacccg aacgcgaagt tcaccacggc attctcagcc
     2581 agggaatagt cggtcgcttc gatcaagcgg tcctgattga catcccccga ccagatgccc
     2641 caaacgcccg gttcaagttc catctggttg gccccgtacg cctttccgga tgcgtcggtg
     2701 aagtcgtagg ccgtgccgga ctccaggaat gggacgggag cagccgacca tgtttcgagg
     2761 tgattccgat tgcggagcac gatgtagtag gagcgttgtg caacattttg agggaagtgt
     2821 acataggacc atccgttcgt gccaagcagg ctggtgccct gatatagact ggcgaatggt
     2881 gtactcgtat ccgcaagtac cacgatgatc gaatccgcaa tggtgtccat gtttatgggg
     2941 tcgatggtgg cccgcatgtt gtcagcgccg gggtcgtaga atccggatag ccacgacttt
     3001 atcgtaaggc cgttgacgaa tgcgaggcag gcaatgttgg cggagtcgct cagcgcggtc
     3061 ccctccgcat ccagtacgct cacgtagtag gtgccgttga ccgcccggat ggaatcgaca
     3121 tttgctccgg tggaccattg gatgaagtat ggaggttgtc cgccactgat tgccgcaatg
     3181 gccagtccgg ttccgttact gtcacaactt gccggtaaag cgaaggcatt caacgtcagc
     3241 ctgccgatgt ctagctgggc tacattgtcg gtggaattaa attctattgg agtacagttt
     3301 aaaagtcttc caatgatcgt cgtacgtgct tcggcgacaa accaggggat ggagaaattc
     3361 gctgattcgt tactatccag attcaaattt cgcgttgcaa tgagaacttc aaaatttgaa
     3421 ttatcgagat aatacagtcc gaatagtccg gtacaccggg tatagccgtt gttctgtacg
     3481 atggcgttga tattgatctg tgcgcctggc gaaggattcg tattcgttgt ggcgaagaag
     3541 gtaaaccgaa gattgggtga ccttcccact acgatggcgc gtgtggcctc attatttgtt
     3601 tcgtcggatt cgctgaggac atcatcgtga tcaacgatcg agcggatcac atgcacgccc
     3661 cggatctgag atgaccaggt ctgtgccagc cggactgtgt tgaacgttcc gttcgtaagc
     3721 ggtgccacga ggatggagtc cagatcactg gtgtcgccgc ggacgagaag ttccaggcta
     3781 tcgggacgat aggctccgat attctcatag gtgaggtcca gggttacttg ctgcccgggc
     3841 tccgggttca ggatgtcggg agcaatgtac tgcgataaca cacggtaatc cggtgagttg
     3901 ctgatgttaa ccgggatcag gaggacatta tccgcttcgt tgcattccgg gtattggttc
     3961 gcggggtcaa tggtaatgcg tatcgtgtag gacccggtct gagggaagga gtagggcaat
     4021 ggcagttgta cgtagatcgg acagccgcag gtgccgggca ggtatttcga gatgttaccg
     4081 gccagtgccc agcctgagat tcccggcccg ctgacctcga atcgtatggt cgcgttgccg
     4141 gcctggtaaa gtcctgcatt gaagacgttc gtggcgagcg taacgggcgc gtttaccgga
     4201 taggaactgt tgatcagcgc attctgcgaa ggcggacagt gactatagtt ggataacgag
     4261 aagttatagc acaatggctg ctttacctgg ttgttctcgt ccaactccgg taccactccc
     4321 gaattatcag cgatcaccgt gagtgtgtcg ctgccgtagg gcggcaccgt tgccagaaca
     4381 gatacggtct tgctcgaatc gggttggatg ttgccgttgt aggttccgta atagtgtgta
     4441 ccgttgacgt cgaagtcgac agtgaacggt ccgttcgctg ccagttgtcc gccgttatag
     4501 attgacgcgg atatcaccat gtttcccccg ggagtcgggt cggttggtgt aatgatcacg
     4561 cttccgcaac tgagcatttg cagatcggct ttgcaatcgt ccaggacgaa ttgatcaact
     4621 gcattgttat tgttctccga taattccgta ataaaattag aaggatcaca attcaactcg
     4681 aagcggtaag tgccggttcc tgcgagggta ggcgtgaatg tgatattgct gctggagata
     4741 ccgttgatcg aagggaaagt ctgcgtctgg gtgctttcca gcgtatcgtt ccggtaaatg
     4801 tccagtctgg cctgataggt tcccgaagga acgccaccgg tattttgaat ggagtaaatc
     4861 agcgtgctca gctgacactt caacagggag gattgcccgc gaaggttcat cgctgttggt
     4921 agaagatcgg gtagcgcagg aaggaccgtg atgttcttga tcaggatgtt gttgaattca
     4981 ttgctttccg tcacggagtc cgtggcgtcg caaatggatc ggatgtaggt ttgccctggg
     5041 ttcggaaaac ggatgtagcc aaggtcaata gcataacttc ctcccggagc cagtggcggc
     5101 actgtattca tgatgccgga tggtaatgct gtcggaatct ggatttgttg gatagactga
     5161 cgtgttatac tgtcacagcc accattctca acagaaaagt aaacatgaag cgagtcgcct
     5221 gctaccacta catcggtgat gcccaaggtc gcatgtaggg taatgattcc ggatccctga
     5281 tggagtaaat cacggatgat gttgcaggat ggctcgattt cacctcctcc gccagcccca
     5341 cctccggggt cgttttcgtt attggtagga caggagccgg taagctcaaa gtcggtgatg
     5401 gtgtcgccgg taagggtgaa atctgtaatg ctgacgttca ctgtatacgg tccaggggtt
     5461 gctgccgtcg gaagcgtgat acaatattcc cccgatgaat tggtgtatcc gctggcgctc
     5521 tgtccggttt cgttgatcct acaggtaacc gtcgcgccgg cgcaggagga gtcggtcagc
     5581 ggtatcgccg tattccggta ataagcagtt ccgcaaacgg taacactgcc gcaaacactc
     5641 gccacttccg gattggcgga agcggtgatc atgatgtcgc cgggtaattc gtaaacacca
     5701 ttggtaaagg ggcgtatggc ctggttatcg agctcattcg gttccgaaag ggcgtttgtc
     5761 gcgtcgacga tcacctgcat gggacaccag gccggaactg ccggtgtggt aatgttccat
     5821 acgacatcta cataagggta ggggttgatc ggtgtataga taaactggtc cggataaacg
     5881 atcgtggtgt cgaattgatt ttttaaccgt attgtcaatt gagaagcatt agcagaacct
     5941 ggatagttgt gaattcgtgc tgtgatggtc aacggactgc caggagcggg attcgggtct
     6001 gagaaggtga tgtcgttcgc gaaaatgctc aggtcgggcg caaaggttac aacagggcct
     6061 gcattcggtc cgtatgaagt ggtacaaccg gtcgggtctt ccacaatgaa tgtatgctgg
     6121 tagtagtagg attccggcaa tccgcttgtc tcgcagtagt agtttttacc atcggtcacg
     6181 tcaacgtcca acgggttgct ttgcaacaac gagatcgaac ggtcgttagc gtcggtgaac
     6241 aatccgttgc cttcgaagtc cagcagcaag cggggatagc cgggttgcgg caaatcaccg
     6301 tcaaggtccg tgtaccggat ttcaaagcgg aatcgctccg ccggttcgcc gctggcaggg
     6361 taaacggcac tggcagcaaa tggcacgttg ccgacatagg ccagtaccgg agcgttgttg
     6421 ttactgacgt ttaccgtgag cgtcagtaac aacaacgc
//
LOCUS       DAOTON010000039         6368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_10505_length_6368_cov_3.169492, whole genome shotgun sequence.
ACCESSION   DAOTON010000039 DAOTON010000000
VERSION     DAOTON010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10505_length_6368_cov_3.169492"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="P5292_01390"
     CDS             complement(<1..717)
                     /locus_tag="P5292_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37807.1"
                     /translation="MKRILLFLSALLGLINSAQSSHIMGGELTYDWLGGNDYRVRYTF
                     YRDCAGIPAPNSVVITYGSVGCGATGLSATLYPTPASPVLVAPVCPSAITTCQGGLST
                     GIEQWSYEGTVLLFPCPDWVIEYTECCRNASITNLVNASSENGYFSAFLNNVATPFNN
                     LARFANAPVPFLYTGLTNQINNGAYDLDNDSIVVTLAPALSGPGTSILYNPGFSATSP
                     IMSSPATTVDPANGNMVVTPT"
     gene            918..2270
                     /locus_tag="P5292_01395"
     CDS             918..2270
                     /locus_tag="P5292_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37808.1"
                     /translation="MESKLQELIDALTATEKRWLERDLKRSGADELWSVYKKREKSPR
                     KRGEQPEDRRAESDLVGWIGDWLAVRRLRRHPEMMAWLRMENWIALEADKAAGYEELK
                     FPATEQLDVIHPYSGFWQHAFGSLRLERASRGAGRKGELDFSTVLDPLDAFYLARKLQ
                     LACELINARNVMQVRGELRAMEKVRQMAQEEPFRHDPAIAIYSTIYDTLTEPETPAHF
                     QRLKELVSQWSGRFSVAQQEEFYRYLKNYCVKQINTGASAFIGELFGIYQLYLSQTEL
                     LDETGLAPGEFKNIVTIGLRLKEYDWVRAFLDRELRRLPEKERSNAQRYNLGNYFFHT
                     GQYHAAIRIFQQVEFSDLYYQLDVRAILLKVYFELGEEELFDYHASAFRTFLSRNRKV
                     SDYQRLIYRNFISFASRTLRKQDDPIALKGLLQEISQNRRVADIRWLEDKIGRLTGLE
                     "
     gene            2532..3773
                     /gene="hemA"
                     /locus_tag="P5292_01400"
     CDS             2532..3773
                     /gene="hemA"
                     /locus_tag="P5292_01400"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRS37809.1"
                     /translation="MQSLKVISFTHKNTPLKELNRFFLHEENRKERLAFLKFSVDIDE
                     LLYLATCNRIEFIFSSPTACDKSFLRRFFQYFRPDWSDEELEFALRHGQVYEGEHALR
                     HIYRVASSLDSLVVGEREIITQVRKSYEKAQEEGLTGDLIRLLVKSTITTAKRVYTET
                     KIANNPVSVVSLAERKLRERKLSKDARILVVGTGETNTNLVKYLLKQGFHRFVFFNRT
                     LSNAEKLAKLVRTSTVTAEAYALSGLSNYKGGFDVLISCTGSADPVITMGVYERLLQG
                     ELTEKVLVDLAIPADIEAEVIRSFPVHLIDIAELKAEAERNLSERQGEFLHAERIIEE
                     SIRSFDDLHRTRSLEIRMREVPDKIREIRQKAVSDIFAKDIEGLDEHSREVLDKVIDY
                     LEKKYISVPMVMAKEILLNKQ"
     gene            3843..5417
                     /gene="hemC"
                     /locus_tag="P5292_01405"
     CDS             3843..5417
                     /gene="hemC"
                     /locus_tag="P5292_01405"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013668383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRS37810.1"
                     /translation="MRNPLIIGTRGSELALWQARFVQSLLKDIGLDSELKIIKTQGDR
                     IQHLSFDKLEGKGFFTKEIEEALLNGETDLAVHSHKDLPTEQPAGLIIAAVSGREDPS
                     ERLLIRKEAVDNRKKFSLRKQARVGTSAARRKSQLLAFRPDLILEDLRGNVPTRVQKL
                     RDGQYDAILMAAAGLERLELDLSDLHVERLDPSEFVPAPAQGVLALQIRAEDHELHRS
                     LQALHHPDVQACIALEREVLRLFEGGCHMPVGAYAVPLEEEGRYRLITSRAESWDQSP
                     VGVTVESRHPETMAATAVQRIKGIAPQRVFVSRDLTHESYLRRILEARQFTVDGRALI
                     ETNPVTIGSLPDSEWIFFSSKQAVQHFFRQRPTVDGRRFACVGKSTAEALRKFGHRAD
                     FIGGSTDTRLTGKQFAALIGDARVLFPQAKGSLRSVQQQLVKKGQAIDLVVYETILKN
                     ETPVDPASIYVFTSPSNVEAYFAKNTLPASVKVIAMGHATRSALREQGVQHPHLPDAF
                     DEAGLSRAVFGLSAQA"
     gene            5467..>6368
                     /gene="hemB"
                     /locus_tag="P5292_01410"
     CDS             5467..>6368
                     /gene="hemB"
                     /locus_tag="P5292_01410"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRS37811.1"
                     /translation="MLTIRPRRLRKNPIIREMVAETRLSKHQFIYPYFVIPGSKNRQA
                     IEAMPGIHRFSVDELVRDVETGLKLGIDKVLLFGVGEAKAEDAHSAYHDHSVVAEAVR
                     ALKGAFGEQLYVITDVCLCAYTTHGHCGLIEHDYVQNDPSLEVLARMALKHAECGADM
                     VAPSDMMDGRVAAIRSKLDANGFSDTPIMSYSVKFASAYYGPFREAADSAPGKGDRKS
                     YQMDFRNGRESVREALLDEAEGADMLMVKPALAYLDVIRSVRESSRLPLACYNVSAEY
                     SMVKAAAKAGFIDEQRIVLENMHA"
BASE COUNT         1489 a         1658 c         1831 g         1390 t
ORIGIN      
        1 cgtaggagtg accaccatgt tgccattcgc ggggtccacc gtcgtcgccg gtgagctcat
       61 gatgggtgag gtggcagaga agcccgggtt gtagaggatc gatgtgccag ggcccgataa
      121 tgcaggagcg agtgtgacta cgatggagtc gttgtcgaga tcgtatgcgc cgttgttgat
      181 ctggttggta agtccggtgt agaggaaagg aaccggtgcg ttggcgaaac gagccaggtt
      241 gttgaatggc gtggccacgt tgttcagaaa cgcgctgaag tacccatttt cggaagaggc
      301 gttcaccagg ttcgtgatcg atgcgttacg gcaacactcg gtgtattcga tcacccagtc
      361 cggacaaggg aacagcagta cggttccttc gtaggaccat tgctcgatac cggtggataa
      421 tccgccctga caggttgtga tcgcggaagg gcaaacaggt gccaccagaa ccggtgaagc
      481 cggtgtagga taaagcgtcg cagagaggcc ggtagcgcca caaccaacgg agccgtaggt
      541 aatgactacc gaattaggtg ccggaatacc cgcgcaatcc cgatagaagg tgtagcggac
      601 acggtaatcg tttcctccga gccaatcata ggtcaattcg ccacccatga tgtgggacga
      661 ttgtgcgctg ttgatgaggc cgagtaaggc ggacaggaag agtagaattc gtttcatggc
      721 gctgcgggat aaagtaaggt agttgattat cggggttaaa ttttagaggg cggattaacc
      781 cgatacgaat gtattggagt cgccaggtgc atgcaatttc attttttggt ataattacag
      841 tattatattc ttgtaaaata agtataaaat attgaaatac aagtttataa ctatacaata
      901 gttgtgtata attacgcatg gaatccaagc tacaggaatt gatcgacgcc ttgacggcca
      961 ctgaaaagcg gtggctggag cgtgacctga agcggagtgg cgccgatgaa ttatggagcg
     1021 tctataaaaa acgggaaaaa tcgccccgga agcgtggtga acagccggaa gaccggcggg
     1081 cggaatcgga tctggtgggt tggatcggag attggcttgc ggtgcgccgg ctgcgtcgtc
     1141 atcctgaaat gatggcctgg ctgcggatgg agaattggat cgctttggag gcggacaaag
     1201 cggctggata tgaagagctg aaattccctg cgacagagca actcgatgtg atccacccct
     1261 atagcgggtt ttggcaacat gcattcggca gtctgcgcct ggaacgagcg agccggggcg
     1321 cgggtcgaaa gggagagctg gatttctcga ctgtgctgga cccactcgat gccttctatc
     1381 ttgccagaaa acttcagctc gcgtgtgagt tgatcaatgc gcggaatgtc atgcaagtgc
     1441 ggggagaatt aagggcaatg gaaaaggtcc ggcaaatggc gcaggaggag ccctttcggc
     1501 acgacccggc gatcgcgatc tactccacca tctacgatac gctcacggaa ccggagaccc
     1561 ccgcacattt tcaacgcctg aaagaactcg tcagtcagtg gtcgggtcga ttcagtgtcg
     1621 ctcagcagga ggagttctac cgttacctga agaattactg cgtgaaacag atcaataccg
     1681 gcgccagtgc ctttatcgga gagttgttcg gtatctatca gttgtatctc tcgcaaacgg
     1741 aactgctaga cgagaccggg ctggctccag gcgagttcaa gaacatcgtg acgatcggct
     1801 tgcgcctgaa ggaatacgat tgggtccgcg cattccttga ccgtgaactg aggcggttgc
     1861 cggaaaagga acggtcgaat gcacagcgct ataacctggg taactacttc tttcatacgg
     1921 gacagtacca tgcggccatc cggattttcc agcaggtgga gttcagcgac ctctactatc
     1981 aactggatgt gcgcgcgatc ctgctcaagg tctatttcga attgggggag gaagaactct
     2041 tcgattacca tgcatcggcg ttccggacct tcctttcgcg taatagaaaa gtgtcggatt
     2101 accaacgcct gatttaccgc aacttcatct cgttcgcctc gcgcaccttg cgtaagcagg
     2161 atgacccgat tgccttgaag ggtcttctgc aggagatctc gcagaaccgg cgcgtggccg
     2221 atattcgctg gctggaggat aaaatcggac gcttgaccgg gctggagtga aagcatgaca
     2281 attccatgac ctgatccggg tggtttgcat gaacttcatc ttgctgattg ccaaacatca
     2341 gacggaattc agaattgtac ttcagttttg atgacccttc tctgcaagtc gggtgatgca
     2401 tgtcatgtcc cggctttcac catccatgtt tctttaccgc catgcaggtt aaccaaatcc
     2461 ttcgtgacac aaatatgaca ggggagaggg ccgctttccg acctttgcag ccggaaaaac
     2521 cagcagtcga attgcagtcc ctgaaggtca tttcgttcac gcataagaac actcccctca
     2581 aggagctcaa ccgattcttc ttacatgagg agaaccggaa ggaacgcctc gcattcctca
     2641 agttttcggt tgatatcgac gaactgcttt acctcgccac ctgtaaccgg atcgagttca
     2701 tcttttccag cccgactgcc tgcgacaaaa gtttcctgcg gaggttcttt cagtacttcc
     2761 gacccgactg gagcgatgaa gagctcgagt ttgccctgcg gcatggacag gtgtacgaag
     2821 gtgaacacgc tcttcggcat atttaccgcg tagcttcttc cctggactcc ctggtagtcg
     2881 gtgagcggga aatcattacc caggtccgca agtcgtatga gaaagcgcag gaagaaggcc
     2941 tgaccggcga cctgatccgc ctgctggtga aatcgacgat caccactgcg aagcgggtat
     3001 acacggaaac gaagatcgcc aacaaccctg tttccgtcgt gtctctggcg gaacgcaaac
     3061 tgcgggagcg caaactgtcc aaagacgcgc gcatcctggt agtcggaacc ggagaaacga
     3121 atacaaacct ggtgaagtac ctgctcaagc agggatttca tcgctttgtc ttttttaacc
     3181 ggacgttgtc caatgctgaa aagcttgcca agctggtccg cacatccaca gtcacagcgg
     3241 aggcatacgc gctgagcggg ctttcgaatt ataagggcgg cttcgacgtc ctgatctcct
     3301 gcaccggctc cgccgatccg gtgattacca tgggcgttta cgagcggctg ctgcaaggtg
     3361 agctgacgga aaaagtcctg gtcgacctcg cgattccggc cgatatcgaa gcggaagtga
     3421 tccgctcgtt cccggtacac ctgatcgaca tcgcagaact caaggccgaa gcggagcgca
     3481 acctcagcga acggcagggc gaattcctgc acgcagagcg gatcatcgaa gagtcgatcc
     3541 gcagtttcga cgacttgcac cgcacgcgtt cactggagat ccggatgcgg gaagtacccg
     3601 acaagatccg cgagatccgt cagaaagcgg tatccgatat ttttgccaaa gacatcgaag
     3661 gcctcgacga gcattcacgg gaagtattgg acaaggtgat cgactacctt gaaaagaaat
     3721 acatcagcgt cccgatggtc atggccaagg agatcctgct gaacaagcag tgaacaacgg
     3781 gacggcggta tattcgcacg ccccgattat ctttatcgcc ggtctgtccg gaatttccaa
     3841 ttatgcgtaa tcctttgatc atcggtaccc ggggaagcga actcgcgctc tggcaagctc
     3901 gattcgttca atccttgctg aaggatatcg gtctcgacag tgagttgaag atcatcaaga
     3961 ctcagggtga ccgcatccag cacctcagct tcgacaagct cgaaggcaag gggtttttca
     4021 ccaaagagat cgaagaagca ttgctgaacg gtgagaccga ccttgccgtg cattcgcaca
     4081 aggacctgcc caccgaacag cccgcgggcc tgatcatcgc agccgtttcg ggtcgtgaag
     4141 atccatcgga gcgcttgttg atccggaagg aagccgtcga taaccggaaa aagttctcgt
     4201 tgcgaaaaca ggcgcgtgtc ggcacttccg ctgcacgcag aaagtcgcag ttgctcgcat
     4261 ttcgccccga cctgatcctc gaagatcttc gcggcaacgt tcctacccgc gttcagaaat
     4321 tgcgcgacgg gcagtatgac gcgatcctga tggccgcggc gggtttagag cgactggagc
     4381 tcgacttgtc cgacctccac gtcgagcggc tggatccgtc ggaatttgtt ccagctccgg
     4441 cgcaaggcgt tctggccttg cagattcggg ctgaagacca tgaattacat cgctccctgc
     4501 aagccttgca ccatcccgat gttcaagcct gcatcgcgct tgaacgggaa gtgttgcgcc
     4561 tgtttgaagg cggctgtcat atgccggtgg gcgcctatgc agttccactc gaagaagaag
     4621 gacgttatcg cctcatcacc tcccgggcgg agtcctggga tcaaagtccc gtcggtgtca
     4681 cggtcgaatc caggcatccg gagaccatgg ccgctacggc tgtgcaacgc ataaaaggca
     4741 ttgcaccgca acgagtattc gtcagccggg atctgaccca cgaatcatat ttgcgacgca
     4801 tactggaagc gcgtcaattc acggtcgatg gccgggcatt aattgaaacg aatccggtaa
     4861 caatcggatc acttcccgat tcggagtgga tcttcttttc cagcaagcag gcggtacagc
     4921 atttcttccg gcaacggccg acggtcgacg gtcgacgttt cgcctgcgtg gggaagtcga
     4981 ctgcggaagc attgcgcaag ttcgggcatc gagcagactt tatcggcggt tcgacagaca
     5041 cgcgtttgac cgggaagcag tttgccgctt tgatcgggga tgcgcgtgtg ttgttcccgc
     5101 aagcgaaagg cagtttgcgt tccgtccagc agcagttggt gaaaaaggga caagccatcg
     5161 atctcgtggt ttacgagacg atactgaaaa acgagacccc ggtggatcca gcctccatct
     5221 atgtgtttac cagtccttcg aatgtcgaag catacttcgc aaaaaataca ttaccagcct
     5281 ctgtcaaagt aattgcgatg gggcatgcca cgcgatccgc cttgcgcgaa caaggtgttc
     5341 aacatccgca cttgcccgac gcattcgatg aagcggggct gtcccgcgcc gtctttgggt
     5401 taagcgcgca ggcttgatac tccaaacatc ttgatctgcg tcccattctg aaaaccactc
     5461 cttcgcatgc tcaccatacg tccccgccga cttcgtaaga acccgatcat ccgcgagatg
     5521 gtcgcggaga cccgcttgtc caagcatcag ttcatctatc cgtatttcgt cattccggga
     5581 agcaagaatc gacaggccat cgaagccatg ccgggcatcc atcgcttctc ggtggatgag
     5641 ttggtgcgcg acgtggaaac gggattgaaa ctcggaatcg acaaagtcct gttgttcggc
     5701 gtaggcgaag caaaggcgga agacgcccat tcagcttatc acgaccatag tgtggtggcc
     5761 gaagcggtac gtgcgttgaa aggagctttc ggggagcagt tgtatgtcat caccgacgtc
     5821 tgcctttgcg cgtataccac gcacgggcat tgcgggctca tcgagcatga ttacgtccaa
     5881 aacgatccgt ccctcgaagt gctggcacgc atggcgttga agcacgccga atgcggagca
     5941 gacatggttg ctcccagcga catgatggat ggccgggtcg cggcgatccg aagcaaactc
     6001 gatgcgaacg gtttttccga cacaccgatc atgtcctatt ccgtgaagtt cgcatccgcg
     6061 tattacggtc cgttccgcga ggccgccgat tctgcgcccg ggaaagggga tcgcaagtcg
     6121 taccagatgg atttccgcaa cgggcgcgag tcggttcggg aagccctgtt ggacgaggcg
     6181 gaaggggcgg atatgctgat ggtcaagccg gcactggctt atctggatgt catccggtct
     6241 gtccgggaga gcagccgcct gccgctggcc tgttacaacg tttcggccga atattcaatg
     6301 gtcaaagcgg ccgccaaagc cggatttatc gacgaacagc ggattgtttt agaaaatatg
     6361 cacgcatt
//
LOCUS       DAOTON010000040         6350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_10546_length_6350_cov_3.128991, whole genome shotgun sequence.
ACCESSION   DAOTON010000040 DAOTON010000000
VERSION     DAOTON010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10546_length_6350_cov_3.128991"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="P5292_01415"
     CDS             <1..447
                     /locus_tag="P5292_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRS37812.1"
                     /translation="VIDSMEPVLKSEADQVVAGSELTFEFNFPTALPQQETAVAAPQT
                     EKKQEERVNSIYALNDTPYEDPMDDQLRKSKERIAKLKSLSMKIGNGNVTDLEREPAF
                     RRRNVKLDSIPHSSESNVSRYTLTGEEEGKKSELRPNNSFLHDNVD"
     gene            656..1108
                     /locus_tag="P5292_01420"
     CDS             656..1108
                     /locus_tag="P5292_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRS37813.1"
                     /translation="MALEERITADLKTAMLAKDDAALRGLRAIKQAILLAKTSGGGGT
                     IAEADEVRMLQKLVKQRKESVEIYVQQGREDLAVSEREEIAVIERYLPALMSEAEVRS
                     AVQAIIAQVGATGPGELGKVMGVATKQLAGKADNKLVSQLVKELLSGN"
     gene            1243..1437
                     /locus_tag="P5292_01425"
     CDS             1243..1437
                     /locus_tag="P5292_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRS37814.1"
                     /translation="METGTVKFFNESKGFGFIVSDESKKDVFVHHSGLIDKVRENDRV
                     EFEVTEGRKGLNAVNVKRIQ"
     gene            1587..2543
                     /locus_tag="P5292_01430"
     CDS             1587..2543
                     /locus_tag="P5292_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRS37815.1"
                     /translation="MNQRVAAAIDIGGTNTAIGLVTAEGRLLAKTSIPTTDHPTPERL
                     VEGVAASIQGLLKQTPGTVPVHIGIGAPNGNYFKGTIEFAPNLLWKGIVPLASLFQDR
                     MNLPAVLTNDANAAALGELYYGAARGMKDYLFITLGTGLGSGVVVNGQVVYGHDGFAG
                     ELGHTIVIQHGRPCGCGRRGCLEQYCSSTGLVRTYAEIMRNTGAEKRHPDGEPDARGI
                     YELALAGDEDAFYAFNYTGEMLGFALANAVAITGPEAIFLFGGIAAAGELLFNPTQIS
                     FEKNLLNIYKHKVKLLPSGLPENDAALLGAASLAWNEWKTES"
     gene            2525..4825
                     /locus_tag="P5292_01435"
     CDS             2525..4825
                     /locus_tag="P5292_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 protein"
                     /protein_id="HRS37816.1"
                     /translation="MENRKLMYCLILTLGIFFMDHTVFAQGTDLPLVPRPESIKRGEG
                     VFRFNANTELLFTDAKLGSEAGLFATLIEQLYGFRPVSKTAAASRPNSIYLSLDGNAS
                     LGNEGYRLTVSTGEVVIQARAAAGIFYGLQTLYQLMPAGKVISAEVPVVEIEDVPRFT
                     WRGMHLDVCRHFFTKQEVFRYLDHLARYKMNRFHWHLTEDQGWRIEIKKYPRLQVVSA
                     WRNGTRIGHYSESPERYDTIRYGGYYSQDDVREVVAYAAQRHIEVIPEIEMPGHALAA
                     LAAYPELSCTGGPFETGRTWGVFQDVFCTREETFTFLQDVLTEVCALFPGKYIHIGGD
                     ECPKDRWKSCPHCQARMKELGLADEHALQSWFVQRMEKFVATKGKRIIGWDEILEGGL
                     APDATVMSWRGYAGGMDAARQGHDVIMAPTAYCYLDYYQSNNPGEPLSIGGYLPIDMV
                     YRFEPVPDALSASEGKYILGTQGNLWTEYIPNFRQVEYMALPRMIALAEVAWSPKAGK
                     DFEDFSRRLVSHMKLLDLLKVNYSKAICDVRLESLPLERSQLAISLSSSYPSGQIRYS
                     MDGKDPDLRSPLYERPIPIGQSAGVKAAMFDGGRRIGSVASQVYRLNRATASKVQLAN
                     PPAEEYANGGAFRLVDGVTGPIPWFGSDWLGFSGTDLDATIDLGVAQEIRRVAVDFLE
                     DRGSWIWYPTSVEVSVSQDGRNFTPVGSWTKETAGTVDRWCTIKFPPTAAQYVRIVAR
                     NAGVIPDGNAGAGRPAWLFADEIQAE"
     gene            4837..5820
                     /locus_tag="P5292_01440"
     CDS             4837..5820
                     /locus_tag="P5292_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HRS37817.1"
                     /translation="MALPRRRFLQLSSFAAAFLAGGRSWARATSPLVTADGPIVLSTW
                     DHGIPANVAAWDVLSRGGRALDAVEAGVRVPEADPKVQSVGYGGLPDRDGRVTLDACI
                     MDERAGCGSVAFLEHIMHPISVARLVMEKTPHVMLVGEGALKFALDHGFKKEELLTPE
                     SKKAWEEWLKTSEYKPVVNWENHDTIGMIALDAKGDLSGACTTSGMAYKLHGRVGDSP
                     IIGAGLFVDNEVGAATSTGVGEEVIRICGSHLVVELMRQGNAPEDACRIAVERIVKNQ
                     PEKSKNIQVGFLALNKHGEYGAYCLQPGFNYAVKSGRVENKLFDGKSYYKE"
     gene            5817..>6350
                     /locus_tag="P5292_01445"
     CDS             5817..>6350
                     /locus_tag="P5292_01445"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HRS37818.1"
                     /translation="MSVLIEVAAGNLESVIAAAEGGADRIELCSALSAGGLTPSPGFA
                     LEARAITRLPIFAMIRPREGDFFYTESEFQSMMHDIRILKETGVDGFVFGALTESGRV
                     DEENCKALLTLCNPLPVTFHRAFDRAADPMQSLEAIIALGFSRLLTSGQADSAWQGRS
                     LIRELVLAAGDRIAILAG"
BASE COUNT         1478 a         1580 c         1815 g         1477 t
ORIGIN      
        1 gttatcgatt ccatggaacc ggttttgaaa tcggaagctg accaggtggt agccggcagt
       61 gagttgacct tcgagttcaa tttcccgacg gcattgcccc agcaggaaac cgctgtagct
      121 gctcctcaaa cggagaagaa gcaggaagag cgtgtgaatt caatctacgc cctgaacgat
      181 acgccgtacg aggacccgat ggacgatcaa ctccgtaaat ccaaggagcg tatcgcgaag
      241 ctgaagtcgc tcagcatgaa gatcggaaat ggtaacgtga cggacctgga gcgtgagcct
      301 gctttccgtc gtcgtaatgt gaagcttgat tcgataccgc attccagtga atcgaacgtc
      361 tcccgatata ccctgacggg cgaggaggaa ggaaagaaat cggaattgcg accgaacaac
      421 tccttcctgc acgataacgt cgactaagat ttttcaacgc actactccgg acccggatca
      481 ctccgcttcc ccgatacccc gactccgcgt ccgcgtagct ttccttcaat agtttttcca
      541 gtgtaaaaac cctcccatcc ccagggaggg tttttttatt tatcgtgcct ttatccatcg
      601 tctcctatgg aaattctaac tttgggattc ccaacaaaac ctgacagcat tcatcatggc
      661 acttgaagaa agaataaccg ccgatctgaa aacggctatg ctcgctaagg acgatgccgc
      721 attgcgaggg ctccgtgcga tcaagcaggc cattctgctt gcaaagactt ccggcggtgg
      781 cggaaccatc gctgaagccg atgaggtcag gatgttgcaa aaactggtta agcaacgaaa
      841 agagtctgtc gagatctacg tacagcaggg cagggaagac ctggccgtct cggaacgcga
      901 agagatcgca gtcatcgagc ggtatctccc ggctctgatg agtgaggcgg aagtccgctc
      961 ggccgttcag gcgatcattg cgcaggtcgg cgctaccggt cccggggaac ttggtaaggt
     1021 catgggggtg gccactaaac aactggctgg aaaggccgat aataagctcg tttcgcagct
     1081 cgtcaaggaa ttactctccg gaaattgagt tttttagggg ggctgacttg cgcggtattc
     1141 taagaatatt acctttggat agcccgcata tcaggaacaa aacccaatgc tctgatcatt
     1201 gttcgcgggc gatggtgttc agggtgagta aaataccaat tcatggaaac cggaacggtt
     1261 aaatttttta atgaatccaa aggttttggg ttcattgtgt cggatgaatc gaagaaggat
     1321 gtcttcgttc atcacagcgg actcatcgac aaagttcgtg agaacgacag agtagaattc
     1381 gaagttaccg aaggacgtaa aggtctcaat gccgtcaacg tcaaacgaat tcagtaattc
     1441 cagaaactac actgaacgag cgggagtcct cagggattcc cgctcgtttt tttatacagg
     1501 ttgatcgcct tttcccagct tgtcgctatt tttcggatgt ttggcaaagt gacttcaccg
     1561 ggttcctacg gacggatccg aattccatga atcaacgagt agctgcagcg atcgatatag
     1621 gagggaccaa cacggccatc ggactggtaa cagccgaggg gcgtttgctg gcaaagacca
     1681 gtataccgac aaccgatcat ccgacgcctg aacgactcgt ggaaggagtt gccgcatcca
     1741 ttcagggtct gctgaaacaa actcccggta ctgttccggt tcatatcgga ataggggcgc
     1801 ccaacggaaa ttactttaaa ggtacgatcg agtttgcccc gaacctcctc tggaagggaa
     1861 ttgttccatt ggcaagtttg ttccaggatc gaatgaacct gccggcagtg ttgaccaacg
     1921 atgccaacgc tgcggcattg ggtgaacttt attatggggc agccagggga atgaaagatt
     1981 acctcttcat caccctggga accggtttgg gcagcggcgt cgtcgtgaat gggcaagtgg
     2041 tctacggtca cgacggattc gccggtgagc tcgggcatac catcgtgatc cagcatggcc
     2101 gtccctgtgg ttgcggccga cgcggatgcc tcgaacaata ttgcagttcg acgggcctgg
     2161 tcaggaccta tgctgaaata atgcgcaata ccggagccga gaagagacat ccggatggcg
     2221 aacccgacgc acgggggatt tatgaattag cacttgccgg cgatgaagac gctttctacg
     2281 cattcaatta caccggtgaa atgctgggtt tcgcgctcgc gaacgcggta gccatcaccg
     2341 gcccggaagc cattttcctt ttcggcggaa ttgcagcggc aggagagctg ttgttcaatc
     2401 ccacgcagat cagtttcgaa aagaacctac tcaatatcta taagcataaa gtgaaactat
     2461 tgcccagcgg gttgcccgaa aacgatgcgg ctttgctggg agcagcttcc cttgcctgga
     2521 acgaatggaa aacagaaagt tgatgtattg cctgatcctg acactcggga tcttcttcat
     2581 ggatcatact gtctttgctc aaggaacgga cttgccgctg gttcccagac cggaatccat
     2641 caaacggggt gagggagtat ttcgcttcaa tgcgaacacc gagctcttgt tcacggatgc
     2701 caaactgggc tccgaagcgg gattgtttgc gaccttgatc gaacagctgt atggttttcg
     2761 tccggtctca aaaacagccg cagcttcccg ccccaactca atctacctgt cgctggatgg
     2821 aaacgcttca ttgggaaatg aaggctatcg actgacggta tccacaggcg aagtggtcat
     2881 tcaagccagg gctgcagccg gtattttcta tggccttcag acgctgtatc agttgatgcc
     2941 ggccgggaaa gtgatcagcg ctgaagtgcc ggtagtggag atagaagatg tgccacgttt
     3001 cacgtggcgg ggaatgcacc tcgatgtttg caggcacttt tttaccaagc aggaggtctt
     3061 ccgttacctg gatcacctgg ctcgctataa aatgaaccgg ttccactggc acctgaccga
     3121 agatcagggt tggaggatcg agatcaagaa gtatccaagg ttgcaggtag taagcgcatg
     3181 gcggaacggt acgcgcatcg gacattacag cgagagtccg gaacggtacg atacgatccg
     3241 ttatggaggg tattattccc aggatgatgt tcgggaagtg gtcgcctatg cagcgcaacg
     3301 gcacatcgaa gtgattccgg agatcgaaat gcccggacat gcgcttgcag ctttggccgc
     3361 gtacccggaa ttgtcttgca ccggcggtcc gtttgaaacc ggtcgtacct ggggcgtttt
     3421 tcaggatgtg ttctgtacac gtgaagagac gtttaccttc ctgcaggatg tgctgaccga
     3481 ggtgtgcgcc ttgttcccgg ggaaatacat ccatatcgga ggtgatgaat gtccgaagga
     3541 tcgctggaaa tcctgcccgc attgccaggc gcgcatgaag gagctgggtc ttgcagatga
     3601 acatgccttg caaagctggt ttgttcagcg gatggaaaag ttcgtcgcga caaagggaaa
     3661 acggattatc ggatgggatg aaatcctcga aggaggtctg gctcccgacg cgacggtgat
     3721 gtcttggcgt ggctatgctg gtggtatgga tgcagctcgt cagggacatg atgtgatcat
     3781 ggcgcctaca gcctattgtt acctcgatta ttaccaatcc aacaatccgg gtgaacccct
     3841 gtcgatcggt ggttaccttc caattgacat ggtctatcgt ttcgaaccgg ttcccgatgc
     3901 tttgtcggca agcgaaggca agtacattct gggaacacag gggaacttgt ggacggagta
     3961 cattccgaat ttcaggcaag ttgagtatat ggcacttccc cgcatgatcg ccctcgcaga
     4021 agttgcctgg tcgccgaaag ccggaaagga cttcgaggat ttttctcgca ggttagtgtc
     4081 ccacatgaaa ttactcgacc tgctcaaggt gaattacagt aaggcgatct gtgatgttcg
     4141 tttggaatca ctcccacttg aacgatcaca actggcgata tcccttagtt caagttaccc
     4201 ttccggacag attcgttatt cgatggatgg gaaagatccg gatttgcgat ctcctttata
     4261 cgagcgtccg attccgattg ggcaatcagc aggcgttaag gctgctatgt ttgatggagg
     4321 acgtcgtatc ggcagtgttg ccagtcaggt ttaccggctt aatcgtgcaa ccgcctctaa
     4381 agtacaactt gccaatccgc ctgcggagga atatgccaac ggcggcgcgt tccgattggt
     4441 ggacggagta accggcccga ttccgtggtt cggttctgac tggcttggat tctcgggaac
     4501 cgacctggat gctacaatcg atcttggtgt agcacaggaa atccggcgtg tggcggttga
     4561 ctttctggaa gatcgtggtt cctggatctg gtatccgaca tccgttgaag tgtccgtcag
     4621 ccaggatggc aggaacttca caccggttgg aagttggacg aaggaaactg ccggaacggt
     4681 tgatcgttgg tgcaccatta agtttccgcc gaccgctgcg cagtatgtcc gcatcgtcgc
     4741 gcgcaatgcc ggtgtcatcc cggatggaaa tgcaggtgcc ggcagacctg cctggttgtt
     4801 tgcggatgaa attcaggctg aataataata ctccacatgg cacttccacg tcgcaggttt
     4861 cttcaattga gttcgtttgc cgccgcgttt ctggcaggcg gtcgttcatg ggccagggcc
     4921 acatcgccat tggtaactgc tgatggcccc atcgtacttt caacctggga tcatggtatt
     4981 ccggcgaatg tcgcggcctg ggatgtcttg tcgagaggcg gacgagcgct cgatgccgta
     5041 gaagccgggg tgcgcgtacc cgaagcggac ccgaaagttc aatccgtcgg ctacggcggt
     5101 ttgccggatc gcgacggacg tgttaccctc gacgcctgca tcatggacga acgcgccggt
     5161 tgcggatcgg tcgcattcct ggaacacatc atgcatccga tcagtgtagc cagattggtc
     5221 atggagaaga ccccgcatgt catgttggtt ggagaaggcg ccctgaagtt cgccctggat
     5281 catggattca agaaggaaga actgttgacg ccggaatcaa aaaaggcgtg ggaggaatgg
     5341 ttgaagacgt ccgaatacaa accggttgtc aattgggaaa atcatgatac gatcggcatg
     5401 atcgcgctgg atgcgaaagg tgacctttcc ggcgcatgca cgaccagtgg tatggcgtac
     5461 aagctccatg gtcgggtcgg agattctccc atcattggcg ccgggctgtt tgttgacaat
     5521 gaagtcggcg ctgccacttc tacgggagtc ggagaggagg tcatccgcat ttgcggatcg
     5581 cacctcgtgg tggagcttat gcggcagggg aatgctcccg aagacgcatg ccgaatcgcg
     5641 gttgaacgga tcgtaaagaa tcagccggaa aagtcgaaga acatccaggt tggtttcctt
     5701 gccttgaaca agcatggtga atatggcgct tattgcctgc agcccggttt taactatgcg
     5761 gtcaagtcgg gccgtgtaga gaacaaactc ttcgacggaa aatcatatta caaggaatga
     5821 gtgtattgat cgaagtagcg gcgggaaacc tggaatccgt gattgccgcc gcggaaggcg
     5881 gtgccgaccg tattgaattg tgttccgcac tctctgccgg aggcttgaca ccctctcccg
     5941 gctttgccct ggaggcccgc gccattacgc gtcttccgat ctttgccatg atccggccac
     6001 gcgagggaga ttttttttat acggagtccg agtttcaatc catgatgcac gatatccgca
     6061 ttctcaagga aaccggcgtc gacggattcg tcttcggagc cttgacggaa tccggacggg
     6121 tcgacgagga gaactgtaaa gcgctgttaa cgctttgcaa cccattgcca gtcacttttc
     6181 accgggcatt cgaccgtgcc gccgacccca tgcagagcct ggaagcaatc atcgcgctcg
     6241 gcttttcacg cttgctgacc tccggtcagg ccgatagtgc ctggcagggg cgatcgttga
     6301 tacgtgagct ggtcctggca gcaggagatc gtatagccat tctcgcaggt
//
LOCUS       DAOTON010000041         6339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_10570_length_6339_cov_3.490770, whole genome shotgun sequence.
ACCESSION   DAOTON010000041 DAOTON010000000
VERSION     DAOTON010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10570_length_6339_cov_3.490770"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1381
                     /locus_tag="P5292_01450"
     CDS             107..1381
                     /locus_tag="P5292_01450"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRS37819.1"
                     /translation="MLVFKFGGASVKDADAVRNVAAILAEYKREQLVVVVSAMGKTTN
                     ALEAVVDAYMNRSSDLADKLQVVREFHEQILDGLGFEANHPVRTEINNVFVEIDWVLE
                     EEPSRGYGYSYDQIVAQGELLSTRIVSAYLEKAGYANTWLDARDVIQTDNNYREAKVN
                     WDTTTKLVRWRVPELMKKHNIIITQGFIGATSENFTTTLGREGSDYSAAIFSFCLDAQ
                     EMTVWKDVPGVLSADPKFYDDADKLEQISYHDAIELTYYGATVIHPKTIKPLENKGIP
                     MRVRSFKAPQEKGTSIGNYQATKPLVPCFIFKTDQILLSISAKDFSFIAEDNLSRIFS
                     LFAEHRAKVNLMQNSAISFSICLDNDPHKVPMLLEALGRDFRVLYNDQLKLVTVRHYY
                     PSTIEQLTKGKTILLEQRSRQTAQVVIRENEK"
     gene            complement(1425..2417)
                     /locus_tag="P5292_01455"
     CDS             complement(1425..2417)
                     /locus_tag="P5292_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS37820.1"
                     /translation="MPDYSELGGKLSDMRVLFIDTVHPALRQGLERAGFVCVDASGWS
                     REELLRQCGDAVGMVIRSRIRIDREFLDAAVALKFIARAGAGMESIDVPYAEAKGVSC
                     LNSPEGNRDAVGEHALGMLLMLLNNLGRADRQVRSGEWQREANRGTELGGKTVGLIGY
                     GNMGGAFARKLVGFDCEVLAYDKYRKNYSDPYAKESDLFELYESCDIVSLHVPLAPDT
                     EYMVNAGFLDRFRKSIVLINTARGKCVRTDDLVAAMKAGKVTGACLDVLEYEDLSFEK
                     FDPRTAGFLDSDTWKYLAGSDRVVLSPHIAGWSFESHERISRVLLEKVISLGLI"
     gene            complement(2432..3217)
                     /gene="rsmA"
                     /locus_tag="P5292_01460"
     CDS             complement(2432..3217)
                     /gene="rsmA"
                     /locus_tag="P5292_01460"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRS37821.1"
                     /translation="MSQPVRAKKHLGQHFLIDEDAAGRIVGALDANGYRDVVEIGPGT
                     GVLSKYLLKREDIRTILLDIDTESITFLRKHYPAHSEQIVEADYLQWNPATIFSGQFA
                     VIGNFPYNISTQILFRVLEFRDRVPQVVGMFQEEVAVRIASRPGNRDYGILSVLMQAW
                     YDVQLLLSLPQEAFQPPPKVRSAVIVCKRKDTEVPSCDEVLFKRVIKAAFNQRRKTLR
                     NALSSLVPKEQMAGLPYLELRAEALSWQQFEILTNAVAQLPGN"
     gene            complement(3220..3525)
                     /locus_tag="P5292_01465"
     CDS             complement(3220..3525)
                     /locus_tag="P5292_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HRS37822.1"
                     /translation="MIIYNVTVNIDDSVHDEWLHWMRSVHIPDVLATGLFSGNRMLRV
                     MSQEESGGTTYSIQYSCASMEDFQRYEREFAEKLRADALRRYPNKFVAFRTLLEVID"
     gene            complement(3534..5387)
                     /locus_tag="P5292_01470"
     CDS             complement(3534..5387)
                     /locus_tag="P5292_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS37823.1"
                     /translation="MTLHFRQRLLRSLACLTLLAVFFVGGTSTVTAQQNTDRQLAEQY
                     LNSSEFEKAAELYDRLFDKDPFLTYGGYLRALLALKRFEDAEKLVKKMTRKSPENPQY
                     LVDLGFVYEAAGSGDKAKGQYDKAIRSLKPDQGYVMMMGSAFTQRQQWDYALQVYQYG
                     QKSLREQYGFFFESAEVYFQKGDAQGMIDQYLDAVADNPQMQQNILNILQARVGYDPE
                     NGRADVLRQSLLRRIQKNPDQPVYAEMLIWLFIQQKDFSSAFIQAKALDKRLREDGTR
                     VITLGNLAMANQQYDAAIQCYEYVVSKGPDNGQYLPARMELLQARNRKLTSGNTYTRP
                     ELVQLESDYLSTLNELGRTGRTAQLVRGLAHLRAFYLDQPDSAASDLEAAIDLPGISR
                     QSQAECKLELGDILLFIGNVWDSDLLYAQVDKDFKNDPLGQEAKFRSARLDYFRGDFL
                     WAQSQLDVLKSATSQLIANDALALSLLISDNIGLDSTYDALNLYARADLLEYRNKQND
                     AMTVLDSLLKEYPDHSLVDEAWFKMAEIEDRRRNWSAEDSLYKEILQRFPESVLADDA
                     LYRRAELYENKLKDTTKAMELYQELLTNYPGSLFAADARKRYRTLRGDLVN"
     gene            complement(5387..>6339)
                     /gene="serS"
                     /locus_tag="P5292_01475"
     CDS             complement(5387..>6339)
                     /gene="serS"
                     /locus_tag="P5292_01475"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRS37824.1"
                     /translation="PNLPSEKVPKGRTPEDNEVVHQEGEIPVLPAGAKPHWELAAQYD
                     IIDFELGVKLTGAGFPVYKGKGARMQRALINYFLDKATEAGYKEMQPPILVNKDSGFG
                     TGQLPDKDAQMYHVGLDEFYLIPTAEVPVTNIYRDVIVKTEELPIRMCAYTPCFRREA
                     GSYGKDVRGLNRLHQFDKVEIVQIEHPDRSYETLDGMVKHVTGLLRALQLPFRILRLC
                     GGDMSFASALTYDFEVYSAAQQKWLEVSSVSNFETFQANRMKLRFRSGSDKPQIAHTL
                     NGSALALPRILAALLEKYQTPDGVVIPDALRAYTGFDRIN"
BASE COUNT         1452 a         1874 c         1625 g         1388 t
ORIGIN      
        1 agtgcgaccg actgctttcg caagggttat ggaaaaatta tttgcaaatt ttatcctatt
       61 tactgttcac tgtttactgt tcactaaaat cgaataatca atccccatgc tcgtcttcaa
      121 attcggtggt gcttcagtca aggatgcgga tgcggtgcgc aatgtggccg cgattcttgc
      181 cgagtataag cgtgaacaac tggtcgtcgt agtttcggcc atgggaaaaa cgaccaacgc
      241 gctggaggcg gtcgttgacg cgtacatgaa tcgctcctcc gatctcgccg acaaactgca
      301 ggtggttcgc gaattccacg aacaaatact ggacggcctc ggattcgaag ccaatcatcc
      361 tgtacgaacc gagatcaaca atgtgttcgt ggagatcgac tgggtgttgg aagaagaacc
      421 cagtcgggga tacggctata gctacgacca gattgtcgcg caaggcgaat tactttccac
      481 ccgcatcgtg agcgcttacc tcgaaaaagc cggctatgcc aacacctggc tcgacgcgcg
      541 cgacgtcatc cagaccgaca acaactaccg cgaggcaaaa gtgaactggg acaccaccac
      601 caaactggtc cgctggcggg tcccggaact gatgaaaaag cacaacatca tcatcaccca
      661 gggctttatc ggcgccacct ctgaaaactt cacgaccaca ttgggccgag aaggttccga
      721 ttattcagcc gcgatctttt ccttctgcct cgacgcgcag gaaatgaccg tttggaagga
      781 cgtgcccggc gtcctcagtg ccgaccctaa gttttacgac gacgccgaca agctcgaaca
      841 gatcagctac cacgacgcga tcgaactgac ctactacggc gcaacggtca tccatcccaa
      901 aacgatcaaa ccgctcgaga acaaaggcat cccgatgcgg gtacgctcgt tcaaagcgcc
      961 ccaggagaaa ggaacgagca tcggcaacta ccaggcgacc aaacccctgg taccctgctt
     1021 tatcttcaaa acagatcaga tcctgctctc catttcagcg aaggacttct ccttcattgc
     1081 cgaggacaac ctcagccgga tcttctcact cttcgccgaa caccgcgcca aggtgaacct
     1141 gatgcagaat tctgccatca gcttctccat ctgtctcgac aacgatccgc acaaagtccc
     1201 catgctactc gaagccctgg gccgggattt ccgtgtactc tacaacgacc aactgaaact
     1261 ggtgaccgtc cggcactact acccgtccac cattgaacaa ctgaccaaag gaaaaacgat
     1321 cctgctggaa caacgaagcc gccagacggc acaggtggtg attcgggaaa acgaaaagta
     1381 actagcaact gaaagccggc agtatggctt tgtaccgaaa aggctcagat caatcccagc
     1441 gaaattactt tttcaaggag aacacgagat attctttcgt gcgattcaaa actccaaccc
     1501 gcaatgtgcg gcgacaagac gacccggtcg gagccggcca ggtatttcca ggtgtctgaa
     1561 tccaggaatc ccgctgtcct ggggtcgaat ttttcaaagc tgaggtcttc gtattccagc
     1621 acgtcgagac aagcgccggt gactttgcct gctttcatcg cggcaaccag gtcgtcggtt
     1681 cggacgcatt tcccccgggc agtattgatc aggacgatgg acttccggaa acggtccagg
     1741 aacccggcat tcaccatgta ctcggtatcc ggcgccagcg gaacatgcag gctcacgatg
     1801 tcgcagcttt cgtataactc gaacaggtcg gactccttcg cgtacggatc gctgtagttc
     1861 ttgcggtact tatcgtaggc cagtacctcg caatcgaacc cgaccagctt gcgggcaaac
     1921 gctccgccca tattcccgta tccgatcaag cccacggttt taccgcccag ctccgtaccg
     1981 cggttggctt cgcgttgcca ttcaccggat cgtacctgcc gatcggcccg gcccagatta
     2041 ttcaggagca tcaacaacat gcccaacgcg tgttcaccaa cggcatcccg attgccttcc
     2101 ggcgaattca ggcaggacac acctttcgct tccgcataag gcacgtcgat gctttccatg
     2161 cccgcgccgg cacgggcaat gaacttgagc gcgaccgcag catccaaaaa ttcccggtcg
     2221 atgcggatcc ggctgcggat caccatgccg accgcatcac cacattgccg caacagctct
     2281 tctcggctcc acccactggc gtccacgcag acgaatcccg cccgctccag gccttgccgg
     2341 agcgcaggat gaaccgtatc gatgaaaaga acccgcatat cggatagctt accgccaagt
     2401 tcggaataat ccggcatatg taccggcgcg atcagttacc gggaagctga gccacggcat
     2461 tcgtgaggat ttcaaactgc tgccagctca gggcttctgc ccgcagttcc aaatagggca
     2521 acccggccat ttgctctttg ggcaccagcg atgacaaggc attgcgaagt gtcttgcgcc
     2581 gttgattgaa cgccgctttg atgacccgct tgaagagtac ttcgtcgcaa gacggtacct
     2641 cggtatcctt ccgtttacaa acgatcacag cggaacgcac tttcggtggc ggctggaacg
     2701 cttcctgcgg cagcgacaac aacaactgca catcgtacca ggcctgcatc agcacgctga
     2761 gaatgccgta atcccggtta ccaggacgcg acgcgatccg taccgccact tcctcctgga
     2821 acatgccgac cacttgcgga acgcgatcac gaaactcgag tacgcgaaac aggatctgtg
     2881 tcgagatgtt gtacggaaaa ttgccgatta cggcgaactg cccggagaag atcgtagcgg
     2941 gattccattg cagataatcc gcctcgacga tctgctccga gtgtgcggga taatgcttgc
     3001 gcaagaaagt gatggactcc gtatcaatat ccaggaggat cgtccgaatg tcctcccgct
     3061 tcaaaaggta tttgcttaag acgcccgttc ccgggccgat ctcgacaaca tcacgataac
     3121 cattcgcgtc cagcgcgcca acgatccgtc ctgccgcatc ctcgtcgatc aggaagtgct
     3181 gacccaggtg ttttttagcg cgaacgggct gactcatgtt cagtcgatca cttcgagaag
     3241 cgtccggaaa gcgacaaatt tattcgggta gcggcgcaag gcatcggccc gcaacttctc
     3301 cgcgaactcg cgttcatacc gctggaagtc ctccatgctc gcgcaggaat attgaatcga
     3361 gtaggtagta ccgccgcttt cctcctgact catcacacgc aacatccggt ttccggaaaa
     3421 cagtccggtg gccagcacgt ccggaatgtg aacggaccgc atccagtgca gccattcatc
     3481 gtgcacacta tcgtcaatgt tgacggtcac attgtaaatg atcatcgctt cattcaattg
     3541 accaggtcgc cacgcagggt acggtaacgc ttgcgggcat ccgccgcgaa caaactgccc
     3601 gggtagttcg tgagcagttc ctggtagagt tccatggctt tggtggtgtc cttcagtttg
     3661 ttctcataca attcggcccg gcggtacagg gcatcatcgg ccaacacgct ttccggaaat
     3721 cgctgaagga tctccttata gagcgaatcc tccgcactcc agttcctgcg tcggtcttcg
     3781 atctccgcca tcttgaacca tgcctcatcg accagtgaat gatcgggata ctccttcaag
     3841 agtgaatcga gaacggtcat ggcgtcgttc tgcttattgc ggtattccag caagtcggcg
     3901 cgcgcgtaca ggttcagcgc atcgtaggtg gaatcgaggc cgatgttgtc gctgatcagc
     3961 aacgaaagag ccagtgcatc gttggcgata agttgcgacg tagccgactt cagcacatcg
     4021 agttgagact gcgcccagag gaaatcgccc cggaaatagt ccaggcgcgc gctgcggaac
     4081 ttggcttcct gtccgagcgg atcgttcttg aaatccttat ccacctgcgc atacagcagg
     4141 tcggaatccc acacgttgcc gatgaaaagc aagatgtcac ccaactccaa cttgcattcc
     4201 gcctggctct ggcgcgagat ccccggcaga tcgatcgccg cttcaaggtc ggatgccgct
     4261 gaatccggtt ggtcaaggta aaacgcacgc aggtgcgcga gtccccgcac gagctgcgct
     4321 gtacgacccg tccgcccaag ttcattcagg gtcgacagat aatcggactc caactgaacc
     4381 agctccggac gggtataggt attaccgctg gtcagcttcc tgttccgcgc ctggaggagc
     4441 tccatgcgcg ccgggaggta ctggccatta tccgggcctt tgctcacaac gtattcgtag
     4501 cattgaatgg ccgcgtcgta ctgctggttg gccatcgcga gatttccaag ggtgatcacc
     4561 cgcgttccgt cttctctcaa tcgcttgtcc aacgctttcg cctggatgaa ggcggagctg
     4621 aaatccttct gctgaatgaa cagccagatc agcatttccg catagaccgg ctgatcggga
     4681 tttttctgga tacgacgcag cagcgactgc cgcaagacgt ccgctcgtcc gttctcggga
     4741 tcgtaaccca ccctggcctg caggatattg agaatattct gttgcatctg cggattgtcc
     4801 gcaaccgcat ccaggtactg atcgatcatc ccctgggcat ctcctttttg aaagtacacc
     4861 tccgcggact caaagaagaa accatactgc tcgcgcagac tcttttgtcc gtactggtag
     4921 acctgaagcg cataatccca ttgctgtcgc tgggtgaacg cgctgcccat catcatgaca
     4981 tatccctggt cgggtttaag ggagcgaatg gctttatcgt actgaccctt tgctttgtct
     5041 ccggaacccg ccgcctcata cacaaacccg agatcgacca gatactgggg attctccggt
     5101 gacttgcgcg tcatcttttt gacgagcttt tccgcgtcct caaaacgctt caatgccaga
     5161 agggcgcgca gatatccgcc gtacgtgaga aaagggtctt tgtcgaataa ccggtcatac
     5221 aactcggcag ctttctcgaa ctccgaactg ttcaggtact gctccgccaa ttgccggtcc
     5281 gtattctgct gggccgtgac agtacttgtt ccaccgacga aaaataccgc cagcaaagtc
     5341 aggcaagcga gcgagcgtaa cagtcgttgg cggaagtgaa gggtcatcaa ttgattcggt
     5401 cgaagccggt ataggcccgg agcgcgtccg gtatgacaac gccgtccggg gtctggtatt
     5461 tttccagcaa ggcagcgagg atgcgcggca aggccagggc actgccgttg agcgtatggg
     5521 cgatctgggg cttgtccgat ccgctgcgga atcgcagttt cattcggtta gcctggaagg
     5581 tctcgaagtt ggaaacactg ctcacttcca gccatttttg ttgagctgcc gaatagactt
     5641 caaagtcgta ggtcaaagcg gaagcgaaac tcatatcgcc tccacaaagc cgcaggatgc
     5701 gaaacggcaa ctgcagggca cgcaacaaac cggtcacgtg cttgaccata ccgtccagtg
     5761 tttcataaga acgatccggg tgctcgatct gcacgatctc gaccttatcg aactgatgta
     5821 aacgattcag tccgcgaacg tcttttccgt agctgcccgc ctccctgcgg aagcagggcg
     5881 tgtatgcgca catacggatc ggcaattctt ccgtctttac gatcacatcc cggtagatat
     5941 tcgttaccgg cacttccgcg gtcgggatga gatagaattc gtcaagtccg acatgataca
     6001 tctgcgcatc cttgtcgggc aactgtcccg tgccgaatcc ggaatccttg ttgacaagga
     6061 tcggcggctg catttcttta taacccgctt ccgttgcctt gtcgaggaaa tagttgatca
     6121 gggcgcgttg catgcgtgcg cctttccctt tataaacggg aaatcccgct cccgtcaact
     6181 ttacgcccaa ttcaaaatcg atgatgtcgt attgagctgc aagttcccaa tgcggttttg
     6241 ctcccgcggg caataccgga atctcccctt cctggtgaac tacttcattg tcttccggcg
     6301 tacgtccctt cggaaccttc tcggacggca gattcggca
//
LOCUS       DAOTON010000042         6334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_10579_length_6334_cov_4.057175, whole genome shotgun sequence.
ACCESSION   DAOTON010000042 DAOTON010000000
VERSION     DAOTON010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10579_length_6334_cov_4.057175"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..536
                     /locus_tag="P5292_01480"
     CDS             87..536
                     /locus_tag="P5292_01480"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRS37825.1"
                     /translation="MNEKAEKHTRFMREALKEAQRAFELDEVPVGAVVVCQGRVIARG
                     HNLTERLGDPTAHAEMQAFTAATNFLGGKYLDQCTLYVTVEPCPMCAGGGYWTQLGGI
                     VFGTRDEKRGFLRFSKELIHPKTVLTGGVLEDECAELMRAFFRKKRN"
     gene            complement(517..924)
                     /locus_tag="P5292_01485"
     CDS             complement(517..924)
                     /locus_tag="P5292_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS37826.1"
                     /translation="MSDFSFERVLLIDDSEIDVLVNRRLIELTRFARQVEVAVSGEDA
                     LELLRNASEMPDWIFLDMHLPGMNGLEFLEELSVTGYNHSRIILLSVMTRPEDLQKVM
                     SFPGVAGQLDKPLTQQALRDLAMNGRVFNSVSS"
     gene            complement(946..1344)
                     /locus_tag="P5292_01490"
     CDS             complement(946..1344)
                     /locus_tag="P5292_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS37827.1"
                     /translation="MSQSRYPVVMLVDDNEIDNFVNKKLIETEKFGDTVLVHTSAVSA
                     LDDLKKRASEPGQLPDLIFLDIMMPNVDGFGFLEAFDGLPEPVKTRAKVVMLSTSESF
                     KDLNRANQNRYVYKFLNKPLNKAVLDAIRI"
     gene            complement(1479..1913)
                     /locus_tag="P5292_01495"
     CDS             complement(1479..1913)
                     /locus_tag="P5292_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS37828.1"
                     /translation="MNRLKTFILSLFLGISLHSSAQQLNVLDNKVYRVTAYKRGDTGI
                     TSRSNYAEVVPPLSIYIPNAFTPNGDGINDGFQVKGEGIRDFHIYIYNRWGEVIFEST
                     NPKQAWDGTFQGRPVEMETYVYQVFADGLGKKSRTGSVTLIR"
     gene            2132..2548
                     /locus_tag="P5292_01500"
     CDS             2132..2548
                     /locus_tag="P5292_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HRS37829.1"
                     /translation="MPYPEYICAPMRQELTAVGFEELKSPEAVDQAIPNSQGTTLLVL
                     NSVCGCAAGTARPGVKLALNHGKKPSKLLTVFAGQDLEATAQARKYTVPYPPSSPSIA
                     LFKDGKLVHFIERHHIEGRSAEMIAEHLQQAFEEFC"
     gene            complement(2602..3561)
                     /gene="plsX"
                     /locus_tag="P5292_01505"
     CDS             complement(2602..3561)
                     /gene="plsX"
                     /locus_tag="P5292_01505"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRS37830.1"
                     /translation="MRLGVDIMGGDYAPEQTVLGAIQALRELPSAVRLVLIGDRPSIL
                     SVLEREGVAADSFEIVHTTEVIGMADHPTKAIQQKPQSSIAIGFQLLKEGKLDSFASA
                     GNTGAMLVGSMFSVKAIPGILRPAISTLLPKENGGWGLLLDVGVNADCKPEVLQQFAV
                     LGSLYAKHVLNIADPTVGLMSIGEEEEKGNLLVKEAHQLIKDSKDIRFVGNVEGRDLF
                     NERADVIVCDGFTGNVMLKEAESFFSILRRRGIRDPYIDRFDYEDYGGTPILGINSTV
                     IIAHGISKAKAIKNMILLSRTVVEAKLSERIAHALSGSREAVS"
     gene            complement(3665..3856)
                     /gene="rpmF"
                     /locus_tag="P5292_01510"
     CDS             complement(3665..3856)
                     /gene="rpmF"
                     /locus_tag="P5292_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRS37831.1"
                     /translation="MPNPKHKHSKTRRDKRRTHWKAEAPTVAIDPTTGQPHLFHRAHW
                     YEGKLYYKGKVIMEQSAAL"
     gene            complement(3884..4444)
                     /locus_tag="P5292_01515"
     CDS             complement(3884..4444)
                     /locus_tag="P5292_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRS37832.1"
                     /translation="MSPFVEYVPMLPKREYVIEFGALPKGQHEFEFEVDDSFFQQFEG
                     SIISRGHADVLVVLERQQDNLLLLDFTIEGTVTVTCDRCLDDLDLDIVSYNELVVKLG
                     EQAEEQSDDVIVIPSGEHNIDIAQFIYEYVTLAIPMRNVHEEDESGNSGCNPELLKRL
                     GDLQGQHDEEPGTDPRWDKLKGINLN"
     gene            4713..5528
                     /locus_tag="P5292_01520"
     CDS             4713..5528
                     /locus_tag="P5292_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRS37833.1"
                     /translation="MSKNGWKYIFYPFQWVWRSLYFINAVLTFFLFFPLFAILLSSKK
                     WFPLVFRVKKIWAHFILWPVGLFYKIERRSQLDPNRSYIFCPNHSSYLDIMLIYISIP
                     SYFVTMGKAELKKVPLFSRFFRRMNIPVNRKSRIDSHRAFLRAGSELDKGVSIALFPE
                     GTIHHNGPVMGRYKNGPFRLAIEKQVPIVPITFVNNWVLLPDDYYRRIGHPGIAHVIL
                     HEPIETKGMTEADLDTLKQRVYAVIDAPLRERYPEWFKRAPSPTAVIPDSVAG"
     gene            5603..>6334
                     /locus_tag="P5292_01525"
     CDS             5603..>6334
                     /locus_tag="P5292_01525"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRS37834.1"
                     /translation="MSKVTAAITAIGGYVPEYVLTNAELEKMVDTTDEWITSRTGIKE
                     RRILKGEGLGTSDLAVPAVQDMLRKRGIQAEDIELLICATTTPDHQFPATANIICDKL
                     GAKNAFGYDISAACSGFIYALVTGSKFIESGQYKKVVVVGADKMSSIIDYTDRATCVI
                     FGDGAGAVLLEPDTDGYGIRDSILRSDGSGRVYLHQKAGGSVKPASHKTVDAGEHYVF
                     QDGQPVFKFAVTNMADVSAEIMEKNK"
BASE COUNT         1446 a         1710 c         1723 g         1455 t
ORIGIN      
        1 ggatgggagt tataccgcaa atttagcgga ggggtttcct tcaaaagaac gacatttatc
       61 aggaatttca atgtccggac cagaaaatga acgaaaaagc ggaaaaacac acccggttca
      121 tgcgggaagc actgaaagaa gcgcagcgtg ctttcgaact ggatgaggtt ccggttggag
      181 cggtggtcgt ctgccagggt cgcgttatcg cgcgcggtca caacctgacg gagcgtctgg
      241 gcgatcccac ggcgcacgcg gagatgcaag cctttaccgc tgccacaaat ttccttggtg
      301 gtaagtacct cgatcaatgt acgttgtacg tgacggtgga accctgcccg atgtgtgccg
      361 gtggaggcta ttggacgcaa ctgggcggga tcgtcttcgg aacgcgcgac gagaagcgtg
      421 ggttcctacg gttttcaaag gaactcattc acccgaaaac ggtgctcacc ggtggagtgc
      481 tggaagatga atgcgccgaa ttgatgcggg cgttcttcag gaagaaacgg aattgaaaac
      541 gcggccgttc atggcgagat cacgaagcgc ctgctgcgtc agcggtttgt cgagttgtcc
      601 cgcaacaccg ggaaacgaca taactttttg aagatcctcc ggtcgggtca ttaccgagag
      661 gaggatgatg cgggaatggt tgtagcccgt gacggacaac tcctccagga attccagacc
      721 gttcatgccg ggaaggtgca tatcgagaaa gatccaatcg ggcatttccg atgcgttgcg
      781 caggagttcc agcgcgtctt ctcctgaaac cgcgacttca acctggcggg caaaacgcgt
      841 cagttcgatg agccggcggt tcaccagcac atcgatctca ctgtcgtcga tcaacaatac
      901 ccgttcgaag gaaaaatcac tcatgcggca gaggggaaaa atcgattaga tgcggatcgc
      961 gtcgagtacc gccttgttca gcggcttgtt gaggaactta taaacgtagc ggttctggtt
     1021 ggcgcggttg aggtccttga aactttcgga ggtggataac atcaccactt tcgcacgcgt
     1081 cttcaccggc tcgggtaagc cgtcaaaggc ttcgaggaaa ccgaaaccgt cgacattcgg
     1141 catcatgatg tcgagaaaga tcaggtcggg caactgaccc ggttcgcttg cgcgtttttt
     1201 caaatcgtca agtgcggaga ccgccgacgt gtgcaccaaa acggtgtcgc cgaacttttc
     1261 ggtctcgatc agtttcttgt tgacgaagtt gtcgatctca ttatcgtcaa ccagcatgac
     1321 cactggatac cggctttggc tcataggcgg gagagaggga attagggttc gctaaattag
     1381 caaaaagcgg cgaacgtcgg ggctcgcttg gtaaacggcc aatatgaaga aagccatccc
     1441 gggggatggc tttcgaattg ttgatgtggt cacgatgttc agcggatcaa tgttacagaa
     1501 cctgttcgcg atttctttcc cagtccgtcg gcgaatacct gatacacgta ggtttccatt
     1561 tcgaccggtc ggccttgaaa tgtcccgtcc caggcctgct tcgggttggt ggattcgaag
     1621 atcacttcgc cccaacggtt atagatatag atatggaaat cgcggattcc ttcgcccttt
     1681 acctgaaatc catcgttgat tccgtcaccg ttcggtgtaa aggcattagg tatgtagata
     1741 ctcagcgggg gcacgacctc cgcgtagttg gagcggctgg tgattcccgt atcgccccgt
     1801 ttataggcgg tcacccggta taccttatta tccagcacat ttagctgttg tgcactggaa
     1861 tgaagggaaa ttccaagaaa taatgataaa atgaaggttt ttaagcggtt catggccaaa
     1921 ggggggcttt acttgatggc aaaatatccc cgcgcgaagg cctccgcaga ccaatgccgg
     1981 cccgaatctc acaactgtaa gagcctgaac ttttcccgcc ctaaatggtt agtgaataaa
     2041 gaaaaaagct ctaaaaacgt tgaaaatcga acaaaaagat gtttcaacaa cagagttcgt
     2101 acctttgcat ctcctctata aaccctaaaa aatgccttat ccagaataca tctgcgctcc
     2161 aatgcgccag gaactcaccg cagtaggctt cgaagagttg aaatctccgg aagcggttga
     2221 ccaggcgatc ccgaatagcc agggcactac cctactggtt ctcaactcgg tctgtggttg
     2281 tgcagccggt actgcccgac cgggcgtgaa actcgcactg aatcatggta aaaagccgtc
     2341 caaactgctg acggtttttg ccggacagga cctggaagcc acggctcagg ctcgtaagta
     2401 cacggttccc tatccaccct cttctccttc catcgccctt ttcaaggatg gcaaattggt
     2461 acacttcatc gaacgccacc acatcgaagg ccgcagcgcg gaaatgatcg ccgagcacct
     2521 gcaacaggct ttcgaagaat tctgctaaga gaaatggagt aaaaaaaaac gcccggcagt
     2581 accgggcgtt tttttttgtg atcagctgac cgcttcgcgc gaaccggaaa gcgcatgcgc
     2641 gatgcgttcg gagagtttcg cttccacgac tgtgcgggac agcaggatca tgttcttgat
     2701 cgctttcgct ttgctgatgc cgtgggcgat gatgacggta ctgttgattc caagaatagg
     2761 cgtgccgccg tagtcttcgt aatcgaaacg atcgatgtac ggatcacgaa tgccgcgacg
     2821 acgcaggatc gagaagaacg actcggcttc tttcagcatc acattgccgg tgaagccgtc
     2881 gcaaacgatc acatcggcgc gctcattgaa aaggtcgcgc ccttcgacgt tgccgacgaa
     2941 gcggatgtct ttcgaatctt tgatcagctg gtgcgcctcc tttaccagca ggtttccctt
     3001 ctcttcctcc tccccgatgc tcatcaagcc aaccgtcgga tcggcgatgt tgagtacgtg
     3061 tttggcgtat agggaaccga gtacggcgaa ctgctgcaat acttcgggct tgcaatcggc
     3121 gttcacgccg acatcgagca acaaacccca accgccgttc tctttcggca acaaggtgga
     3181 gattgcagga cgcaggatgc cgggaatggc tttcacgctg aacatggagc ctacgagcat
     3241 ggcgccggta ttgcctgcag aggcgaacga gtcgagcttt ccttctttga gcaattgaaa
     3301 gccgatggcg atactcgatt gcggtttctg ctggatggct ttcgtgggat ggtcggccat
     3361 gccgatcacc tccgtcgtgt gtacgatctc aaacgaatcg gccgcaacgc cttcgcgttc
     3421 cagtacggac agaatggaag gacggtcgcc gatgaggacg agtcggacgg ccgacggcag
     3481 ttcgcgcagc gcctggatgg caccgagcac cgtctgttcc ggcgcgtaat cgcctcccat
     3541 gatatcgact ccgagtctca tcgcgtggtg gtgtttgaca gcagtaaaat acgcattgaa
     3601 tgaaaaagca gctgacaggt cgtgcgcgga cttgtcaact gcttggtgtc aaatcgtgat
     3661 cggatcagag ggctgccgat tgctccatga tgaccttgcc cttgtagtag agcttgcctt
     3721 cgtaccagtg tgcacggtgg aaaaggtgcg gctggccggt ggtcgggtcg atggctacgg
     3781 tcggagcttc cgctttccaa tgcgtacggc gcttgtcgcg acgggtcttc gaatgtttgt
     3841 gtttaggatt tggcatggcg ttctactatg atatgcgttg tactcaattc aggttaatgc
     3901 ctttcaactt gtcccagcgg ggatcggtgc cgggttcctc gtcgtgttga ccttgcagat
     3961 caccgagacg tttcagcaac tccgggttgc aaccgctgtt ccccgactcg tcttcctcgt
     4021 gtacgttgcg catcgggatg gccaacgtga cgtactcgta gatgaactgc gcgatgtcga
     4081 tgttgtgttc tcccgaaggg atcacgatca catcgtccga ctgctcctcg gcttgttctc
     4141 cgagtttcac gaccagttcg ttgtaactga cgatgtcgag gtcgaggtcg tcgaggcaac
     4201 gatcacaggt aacggtaacc gtcccttcga tcgtaaagtc aagcagcagc aggttgtcct
     4261 gttgtcgctc cagcaccacc agcacatccg cgtgaccgcg gctgatgatg gagccttcga
     4321 attgttgaaa gaacgaatcg tcaacctcga attcaaactc gtgttgaccc tttggaaggg
     4381 ctccgaactc aatcacatat tcccgcttcg gcaacatcgg aacgtactca acaaagggcg
     4441 acaaaagtac ggaaaatccg gatacgacgc cacctgggag gagcgagatg cgagtacgag
     4501 gatcgaggaa agtgaggttt gttactatct gccatccctt aaaaccagcc ctgatttcta
     4561 ttgataagct aagaatgccc gttttttcga taaaaggctc gttttttcaa cagtcttcct
     4621 tcctcgttcc tcgtcctcgt acctcgtatc tcctttcaaa cgtccctcgt ccctccttta
     4681 gagtaccttt gccctgccta cgggctggcg aaatgtcgaa aaacggctgg aaatacattt
     4741 tttacccctt tcaatgggtg tggcggtcgc tctatttcat caatgccgtt ctcacgtttt
     4801 tcctcttctt ccctttattc gccatcctgc tttccagtaa aaagtggttt ccgctggttt
     4861 tccgggtcaa gaagatctgg gcccatttta tcctgtggcc ggtggggctc ttttacaaga
     4921 tcgagcgacg cagccaactg gacccgaatc gctcctatat cttttgtccg aaccacagtt
     4981 cctacctcga catcatgctg atctacatca gcatcccttc ctatttcgtc accatgggta
     5041 aagccgagtt gaagaaggtg cctctgttca gccgtttctt caggagaatg aacatcccgg
     5101 tgaaccgaaa gagccgcatc gactcgcacc gcgcctttct acgcgcaggt tccgagctcg
     5161 acaaaggtgt cagcatcgcg ctcttcccgg aaggaacgat ccaccacaac ggcccggtca
     5221 tgggtcgtta caagaacggg ccgttccggc tagccatcga aaagcaggta ccgatcgttc
     5281 ccatcacctt cgtcaataac tgggtgctat tgccggacga ttactaccgt cggattggtc
     5341 acccgggaat cgcacacgtc atcctgcacg agccgatcga aacgaaaggc atgacggaag
     5401 ccgatctgga tacactcaag caacgggtgt acgcggtgat cgatgcacca ttacgcgaac
     5461 gctatccgga gtggttcaaa cgtgcgccaa gcccgactgc cgtgatcccc gattctgtgg
     5521 cgggttaagt atggaagtac aggcgcgatt catttgaaca aaaccgggga ttcttacctt
     5581 tggtctcccc acgcaccctc ccatgtccaa ggtcactgcc gcaatcaccg ccatcggcgg
     5641 atatgttccg gaatatgtac tcacgaatgc cgagctcgaa aagatggtcg acaccaccga
     5701 cgagtggatc acgtcccgca ccggcatcaa ggagcgccgg atcctgaaag gcgaaggcct
     5761 gggcacgagc gacctcgccg tgccggcggt acaagatatg ctgcgcaagc gcggcatcca
     5821 ggccgaggac atcgagttgc tcatctgcgc caccaccacg cctgatcacc agttcccggc
     5881 caccgccaac attatttgcg acaaactcgg cgcgaagaac gccttcggct acgacatcag
     5941 cgccgcctgt tcgggtttca tttacgcgct ggtcaccggt tcgaagttca tcgagtcggg
     6001 tcagtataag aaagtcgtcg tggtaggtgc cgacaagatg tcgagtatca tcgactacac
     6061 cgaccgcgcc acctgtgtga tcttcggtga tggtgccggc gccgtgttgc tggagcccga
     6121 tacggatggt tacggaatcc gcgactcgat cctgcgcagc gacggttccg gtcgggtgta
     6181 cctgcaccag aaagccggcg gctcggtgaa gcccgcctcg cacaaaacgg tagacgcggg
     6241 cgaacactat gtgtttcagg acggacagcc cgtcttcaaa ttcgccgtga ccaacatggc
     6301 cgacgtgtct gccgagatca tggaaaagaa caaa
//
LOCUS       DAOTON010000043         6330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_10587_length_6330_cov_3.048765, whole genome shotgun sequence.
ACCESSION   DAOTON010000043 DAOTON010000000
VERSION     DAOTON010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10587_length_6330_cov_3.048765"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..964)
                     /locus_tag="P5292_01530"
     CDS             complement(206..964)
                     /locus_tag="P5292_01530"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS37835.1"
                     /translation="MSEKKFWDKIGGSYADEIFSVFASDKFGRLKKLVEKHAGKRKDA
                     IDFGCGIGRALPLLAPNFRHVLAVDISTELLAIACLYPALNVTYQQADLAVKNTGLPP
                     ADFAFCANVAIGDQPERNYRILENVTKGLKKNGTAVIVVPSVESAGLSKWTLMKLYEA
                     EGKPAYRIPKEDRKLLNGLDLKAVQHGIISIDGNPTKHYQLTELFALFDRGATRIESL
                     DKLEYPWSTEFAEPPASLRAPYPWDWVVEVKRVK"
     gene            complement(1118..2212)
                     /locus_tag="P5292_01535"
     CDS             complement(1118..2212)
                     /locus_tag="P5292_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HRS37836.1"
                     /translation="MAESSIQTEIDAAFLYGRLADHEKDPVISRIFRQMSEIEQGHAA
                     AFAQKAGIPATALPRPSWRAKVLDRIGRIFGYDTVLGALMDTEKSISNAIVRTKQQLN
                     EPVTGNEANHVRILRSILEKEERVSGTQLTRFEKRHRTVGGNAIRAAVLGGNDGLVSN
                     FSLVMGIAGASANEHTVLVTGLAGLLAGALSMSLGEWISVKSSQELYEQQMALEMEEI
                     QANPEGEKKELALIYMAKGIPDEQARAMAEKTMQDTAHAHEVLVKEELGINAEELKGS
                     AMEAALSSFLLFAIGAIIPVLPFFFFTGTRSVLLSVAFSAAGLFLIGAAITLFTGRSV
                     WFSGMRQVIFGLAAAAVTYGIGHLIGVSVN"
     gene            complement(2275..2751)
                     /locus_tag="P5292_01540"
     CDS             complement(2275..2751)
                     /locus_tag="P5292_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37837.1"
                     /translation="MQPFVLYGNRLNIPVTREFKQLVNITIAAGKFILLHPNYDLIRE
                     AMRLEMFEDTRLEDFEVHTWQYEIGEVISYDLEEVLFFYTLLDLSCRIFLCEIGDDLR
                     QMAIDSGETNTDEFNRVRSFFLKQAQEYLIQLRNCYSDNETFRVLQGKIEQLNSLA"
     gene            2877..3458
                     /locus_tag="P5292_01545"
     CDS             2877..3458
                     /locus_tag="P5292_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HRS37838.1"
                     /translation="MLGLKLPTDPRWVNIVETNISEILTDHAYCEQKAATNAINMVIQ
                     FPEYPDLVDAMLALAQEELQHFRQVHDKIQARGFSLGRERKDDYVWELNQFIRKGRER
                     HIVLVDRLLFAAMVEARSCERFRILSEHISDPDLRAFYRDLMISEANHYTLFIGFARQ
                     YGKGEDVDKRWQDFLDYEAQLIAKYGKKETMHG"
     gene            complement(3472..3906)
                     /locus_tag="P5292_01550"
     CDS             complement(3472..3906)
                     /locus_tag="P5292_01550"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRS37839.1"
                     /translation="MSIFKHKTPIEIRFADVDAFGHVNNARFLTYIEMARVKYFDEVV
                     EWGYDWSKEGIILARAEVDYIQPILFKEELFVYTRCSRLGKKSFDLEYQLVVFKDGKE
                     ILKADACTVMVAFDYRAHRSIEVSASWREAIIKYEGANVLQQ"
     gene            complement(4048..5943)
                     /locus_tag="P5292_01555"
     CDS             complement(4048..5943)
                     /locus_tag="P5292_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRS37840.1"
                     /translation="MRFRLSTRNTPRWIIFLIDTSICFLSLMLAYQVRFNFRVPENEI
                     ALWKYAVPTVLGVRIISFLISRVYQGIIRYTSNKDAQRIFYTITAGSVALAVANILSY
                     HSIGFYLVPYSIVIIEYFATVFSMTAFRILVKTVYMEVRNPSGERKKVIIYGAGESGV
                     ITKRTLDRDAGSRYKVIAFVDDNPAKAGKTLEGIRIYRADTELEELLHANVIDHLILS
                     IQNVPAPAKQALVEKCLAFGVNVLNVPPVTTWINGQLSFNQIRNIRIEDLLERPPIRL
                     DTEEIRRQLEGRVILVTGGAGSIGSEVVRQLLPFNPKKVIVLDQAESALYDLELELQE
                     RYKFRQFETVIADVRQIDRMRRVFEAFHPQVVFHAAAYKHVPVMEHNPSEAILTNVHG
                     TRVTADLAHEFGVEKFVLISTDKAVNPTNIMGATKRIAEIYCQSLGTVSATRFITTRF
                     GNVLDSNGSVIPRFRKQIESGGPVTVTHPDITRYFMTIPEACQLVLEAASMGKGGEIY
                     LFDMGTSVRIYDLAKKMIRLAGLTLDKDIRIEITGLRPGEKLQEELLADKETSLPTHH
                     EKILVAKVRPYDFKVIQSDIEELVSLFSTQRNDAIVRKMKQMVPEYVSANSEFEKLDV
                     VTGKFDG"
     gene            6060..>6330
                     /locus_tag="P5292_01560"
     CDS             6060..>6330
                     /locus_tag="P5292_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRS37841.1"
                     /translation="MKRVLFLVPYPQGEAASQRFRFEQYLPALQETGWDVHLSPFLDL
                     RAWKVLYQPGHLDRKAFAILRGYLRRLGDLLKLGRYDAVFIHREAS"
BASE COUNT         1425 a         1654 c         1696 g         1555 t
ORIGIN      
        1 actccagatc gaagagcaag aggagctgcc gtttgaaaat ggtgaagtcg gaaatgaata
       61 atggaagacg ggaaacggaa agtggtaact agaaactggt aactagaaac tagtaactag
      121 aaactggtaa ctggaaatta aaaaccagag atcgagtact aaccaattac taattactaa
      181 ttaccagtca cctgtctcaa ctttctcact tcacccgttt cacctccacc acccagtccc
      241 acggataggg ggcgcgaagg gaagcgggag gttccgcgaa ttcggttgac caggggtatt
      301 ccaacttgtc gagggattca atgcgggtag cgccccggtc gaagagcgcg aaaagctcgg
      361 tgagttgata gtgtttggtc gggttaccat caatggaaat aatgccgtgt tgaacggctt
      421 tcagatcgag tccgttcagg agcttccggt cttccttcgg gatccggtat gcaggtttac
      481 cttccgcctc atacaacttc atcagggtcc atttggatag ccctgcggac tcgaccgatg
      541 gtaccacgat caccgcggtt ccgtttttct tgaggccttt cgttacgttt tcgagaatcc
      601 ggtagttgcg ttcgggttga tcaccgatgg cgacgttggc acagaacgcg aaatcagcag
      661 gagggaggcc ggtgtttttt acggcaaggt cggcttgttg atacgtgaca ttcaaagccg
      721 ggtaaagaca agcgatggcc agcaactcgg tggaaatatc cactgccagt acgtggcgga
      781 agttaggagc cagcaaggga agcgccctgc caatgccgca gccgaagtcg atggcgtcct
      841 ttcttttgcc ggcatgcttt tcgaccagtt ttttcaaccg gccaaatttg tcgcttgcaa
      901 agacactgaa tatctcatcg gcgtaactgc cgccgatctt atcccagaat tttttttcgc
      961 tcatacgtcc agtacttggg ctcaatgtat ccaaaagatc aggattcccg accgctcggc
     1021 cgcagtgttt atctcgatga ctgaactatt ctgtgttcgg ttaacggatc tgcagttggg
     1081 agcgaacgat ttcgaatcaa tagcggttcc tgaaaattca attgacggaa acaccgatca
     1141 gatgtccgat gccataggtc accgcggctg cagccagtcc gaagatcacc tgccgcattc
     1201 cggaaaacca tacggaacgg ccggtgaaaa gtgtgatggc tgcaccgatg aggaaaagtc
     1261 cggccgcact gaaggcgacg ctcagcagga ccgaacgcgt accggtgaaa aagaaaaagg
     1321 gtaacaccgg aatgatggcg ccgatggcaa agagcaggaa ggaactgagg gcagcttcca
     1381 tcgccgaacc tttcagttcc tccgcgttga tccccaattc ttccttgacc agcacctcgt
     1441 gcgcgtgcgc cgtatcctgc atcgtttttt ccgccatggc gcgggcctgt tcgtccggga
     1501 ttcctttggc catgtagatc aatgccagtt ctttcttttc gccttccggg ttagcctgga
     1561 tctcttccat ttccaacgcc atctgttgtt cgtagagttc ctgcgagctc ttcacggaga
     1621 tccattcgcc caatgacatc gacaacgcgc ctgccaatag tccggcaagt ccggtaacca
     1681 ggactgtatg ctcgttcgcg ctggcaccgg ctatgcccat gaccaggctg aagttggaaa
     1741 caagaccatc gttgccaccg agtacggctg cccgtatcgc gtttcctccg accgtccggt
     1801 gacgcttctc aaaccgtgta agttgcgtgc cgctgacccg ttcctctttt tcaaggatgg
     1861 agcgcaggat gcgtacgtga ttggcttcgt tgccggtgac cggttcattc aattgttgct
     1921 tggtacgaac gatcgcgttc gagatgcttt tttccgtgtc catcaaggcg cccagtaccg
     1981 tgtcgtaacc gaagatccga ccgatgcggt ccagtacttt cgctcgccac gaagggcggg
     2041 gcagtgcggt cgcgggtata cccgctttct gcgcgaaagc ggcggcgtgt ccctgttcga
     2101 tctcactcat ctggcggaag atgcgtgaaa tgacggggtc tttctcgtga tcagccagac
     2161 gtccgtacag gaacgcggca tcgatctcgg tctgaatgga agattcggcc ataggaatag
     2221 ttcgtgctca ggcaaccggg cccggaaacg aagttaagca atcagccggt atgcttaggc
     2281 tagggagttc aactgctcga tctttccttg cagtacccgg aacgtctcgt tgtccgaata
     2341 gcaattgcgc agttgaatga ggtattcctg tgcctgtttc aggaaaaaac tacggacacg
     2401 gttgaactcg tcagtattgg tttctccgct gtcgatggcc atctgtcgca agtcatcacc
     2461 gatctcgcac aggaagatcc ggcaactcag gtccagcagg gtgtaaaaga aaagcacttc
     2521 ttccaagtca tacgagatga cttcgccgat ctcatattgc caggtatgta cctcgaagtc
     2581 ctccagccgc gtatcctcga acatctccag ccgcatcgct tcgcggatga ggtcgtaatt
     2641 cggatgcagg agaatgaact tgcctgcggc gatcgtgatg ttcacgagct gcttgaattc
     2701 ccgggttacg ggaatattca aacggttgcc gtacaggacg aagggttgca tgatgctctt
     2761 attacaaaaa tacggcacaa ttggttacta tcatcctttt cggaagacct ccttctgcga
     2821 gtattttctt acagcagttg cttactttta ccccctgatt tttcacccga attcgcatgc
     2881 ttggactgaa acttccgacg gatccgcgct gggtcaatat cgtggaaacc aatatcagtg
     2941 aaattcttac tgaccacgcg tattgtgagc agaaagcggc taccaatgct atcaatatgg
     3001 tcatccagtt tccggaatat cccgacctgg tggatgccat gctggcgctt gcacaagaag
     3061 agctgcagca tttccggcag gtccacgaca agatccaggc gcgtggtttt tcgctggggc
     3121 gggagcggaa agacgattat gtgtgggagc tcaaccagtt tatacggaaa ggacgggagc
     3181 gtcacatcgt tttggtcgac cgtctgctct tcgccgcaat ggtcgaagcc agaagctgtg
     3241 aacgattccg gatcctgtcg gagcatattt ctgacccgga tctgcgagcg ttttatcggg
     3301 atctcatgat ctccgaagcc aaccactata ccttgttcat cggattcgcc cggcagtatg
     3361 gaaaagggga agatgtggat aagcgctggc aggattttct ggactacgaa gcgcaattga
     3421 tcgccaaata cgggaagaag gaaaccatgc acggctgatc agcgtgtcga atcactgctg
     3481 cagtacgttc gctccttcgt acttgataat ggcctcccgc cacgaagccg atacttcaat
     3541 ggatcgatgt gcccggtagt cgaacgctac catgaccgta caggcatccg ctttcaggat
     3601 ttctttcccg tctttgaata cgactaattg atactcgaga tcgaaacttt tcttgcccag
     3661 ccttgaacag cgtgtgtaca cgaagagttc ttccttgaac aggatcggct ggatgtaatc
     3721 cacttcagcc cgcgccagga tgatgccttc cttggaccag tcgtatcccc attctaccac
     3781 ctcgtcgaag tacttgacac gggccatctc gatataagtc aggaatcgcg cattgttcac
     3841 atgtccgaaa gcatccacat ccgcgaaccg gatctcgatg ggggttttgt gtttgaaaat
     3901 tgacataggg ggacgggttt acttccttcg attaagctcc tgcgggcagg tgggcaggtt
     3961 tcgagtttcg agtttccggt tgatcgttgt aaatcaccaa tcaccaatca ccaatcacca
     4021 atcaccaatc acgaatctcc aatctcccta cccatcaaac tttcccgtaa ccacatccag
     4081 tttttcaaac tccgaattgg cggagacata ttccggcacc atttgtttca tctttcggac
     4141 aatggcatcg ttgcgttggg tggagaagag cgatacgagt tcttcaatat cggattgaat
     4201 gaccttgaaa tcgtaaggtc tcacttttgc aaccaggatc ttctcgtggt gagtcggaag
     4261 cgaagtttct ttgtcggcga gtagttcttc ctgcaatttt tctcccggac gaagtcccgt
     4321 gatctcgatc cggatgtcct tgtccagcgt caatcctgcc agtcggatca tttttttagc
     4381 aaggtcatag atgcggacac tcgttcccat gtcgaacagg tagatctctc cgcctttccc
     4441 catggacgcc gcttccagaa ccagctggca ggcttccgga atcgtcatga agtagcgcgt
     4501 gatgtccgga tgggtaaccg ttaccggtcc gccggattcg atctgtttac ggaagcgtgg
     4561 aattacagag ccgttcgaat cgagtacgtt tccgaagcgg gtcgtgatga atcgggtcgc
     4621 agagaccgta cccaacgact gacaatagat ctccgcgatg cgtttggtcg cacccatgat
     4681 gttcgtcggg ttgaccgctt tatcggttga gatcaggacg aatttttcta caccgaactc
     4741 gtgagcaagg tcagccgtga cgcgtgtgcc gtgtacgttg gtcaggatgg cttccgaagg
     4801 gttgtgctcc atgaccggta cgtgcttgta cgctgccgca tggaacacca cctgcggatg
     4861 aaaggcttcg aagacgcgac gcatccggtc gatttgtcgg acatcggcga tcacggtttc
     4921 aaactgccgg aacttgtagc gttcctgcaa ttccagttcc agatcgtata aggcggactc
     4981 tgcctggtcg agtacgatta cctttttcgg attgaacggt aacaattgtc gaacgacttc
     5041 gctgccgatc gatcctgcgc ccccggtcac cagaatgaca cgaccttcga gttgacgacg
     5101 gatctcctcg gtatcgagtc gaatgggtgg gcgctcgagc aggtcctcga tccggatgtt
     5161 ccggatctgg ttgaagctga gctggccgtt gatccaggtc gtgaccggcg gaacattgag
     5221 tacgttgaca ccgaaggcga ggcacttttc cacgagcgcc tgcttggcgg gagccggtac
     5281 gttctggatg ctcaggatca ggtggtcgat gacgttcgcg tggagcaatt cttccaactc
     5341 ggtatcggcg cggtagatcc gaatgccttc cagcgtcttg cccgccttag ccggattatc
     5401 atccacaaag gcgatgacct tgtatcggct acctgcgtcg cggtcgaggg tgcgcttggt
     5461 gatcacgccc gattcaccgg cgccgtagat gatgactttc tttcgttcgc cggaaggatt
     5521 gcgtacttcc atgtaaacgg tcttcaccag tatcctgaat gcggtcatcg agaaaacggt
     5581 tgcgaagtac tcgatgatga cgatggaata ggggacgagg taaaagccga tgctgtgata
     5641 cgacaggatg ttcgccaccg ccagtgcgac ggaaccggcc gtaatggtgt agaagatcct
     5701 ttgcgcgtcc ttgttgctcg tataccggat gatgccctgg tagacgcgcg atatcagaaa
     5761 gctgatgatc cgaacgccca gcacggtcgg cacggcatat ttccataggg cgatctcgtt
     5821 ctcgggaacg cgaaagttga agcgcacctg atacgccagc atcagggata aaaaacagat
     5881 cgaggtatcg atcaggaaaa tgatccagcg cggggtattg cgggtggaaa ggcgaaacct
     5941 catggctgga tgtggactag ctgccgacta accgacgatt taccaaatta accctcaaat
     6001 atactctaat ttctgcgttt tatctgttcc tccggctatc tttgtcgttt gcatcctgcg
     6061 tgaaacgagt actctttctg gtcccctatc cgcaaggtga agccgccagc cagcgatttc
     6121 gctttgagca ataccttccg gctttgcagg aaacgggatg ggatgtgcat ttatctccct
     6181 ttctggactt gcgtgcgtgg aaggtcttgt atcaacccgg gcacctcgat cgaaaagcgt
     6241 tcgcgatcct gcgcggctat ctccgcaggc tgggcgatct gctgaaactc ggtcgctacg
     6301 atgccgtgtt catccaccgt gaagcgagcc
//
LOCUS       DAOTON010000044         6246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_10759_length_6246_cov_3.658698, whole genome shotgun sequence.
ACCESSION   DAOTON010000044 DAOTON010000000
VERSION     DAOTON010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10759_length_6246_cov_3.658698"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="P5292_01565"
     CDS             <1..1255
                     /locus_tag="P5292_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRS37842.1"
                     /translation="WFAKSSGTTAGKSKFIPVSQEALDECHYKGGKDLLSIYCSNYPD
                     TLIFTGKGLTLGGSHRAAKDGSEAFAGDISAILMQNMPFWAQMIRTPDLSIALMDEWE
                     EKIERMARATIQENVTSISGVPTWTAVLANRVLEITGKRNLLEVWPNLELFIHGAVSF
                     TPYREQFRSLCPADSFHYLETYNASEGFFGIQDLRNSEELLLMLDYGVFYEFMPMEEY
                     GKEFPETVGLEGVEIGRNYALVISTNAGLWRYVIGDTVRFTSLDPFRIRITGRTRLFI
                     NAFGEEVIIENTDMAVRIACEKANCLVREYTVAPVYFNQRENGAHEWLIEFERSPDHL
                     DYFKELLDNALKSLNSDYEAKRYRDMALRFPVIREVPQGTFHEWLRMKGKLGGQHKVP
                     RLSNERQHVEEILQLLRERERPIQA"
     gene            1252..2046
                     /locus_tag="P5292_01570"
     CDS             1252..2046
                     /locus_tag="P5292_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37843.1"
                     /translation="MKRLLVILFLFTAGAFRPAEDFHLLHQLPVSGNATVTTDPLGNC
                     YLTVGNQVLKFDPKGKPLSNFAQVDRGELRGVDALNPLKVLLFYPDFAQIILLNTQLS
                     VQSTINLRNLDIQQPWLACSSLNDGYWVFDLQDAQLKKIGLDLQLQFQSGNLNQLLRK
                     RLVPMALLESQESVFLADPEAGILVFDRFGNYDKTLPVPGVRKFQVIGEEVLYLRNDS
                     VFAVNRKTLEEHPVVLPSHEGAVRDFRFEQQELYLLTTDALSIYSF"
     gene            2237..2845
                     /locus_tag="P5292_01575"
     CDS             2237..2845
                     /locus_tag="P5292_01575"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRS37844.1"
                     /translation="MAIAGNIGAGKTTLTNLLAKTMKWEPHFEDVDDNPYLNDFYEDM
                     QRWSFNLQIYFLNARFEQVMKIRQSGKTVIQDRTIYEDANIFAPNLHSMGLMTTRDFN
                     NYQSLFSLMTKFISPPDLLIYLRASVPTLVNQIGKRGRDYENSIRIDYLKRLNERYEA
                     WISTYEQGKLLIIDVDDLNFADNPEDMGKIVNKINAEINGLF"
     gene            complement(2983..3699)
                     /locus_tag="P5292_01580"
     CDS             complement(2983..3699)
                     /locus_tag="P5292_01580"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HRS37845.1"
                     /translation="MKKTLYTLLILTFALSAAAQYGGSVIHLHQYNNKRFALRVDGVP
                     FGSGERNYTVTGLQPGYHMLEATTWSGNGHHGHYPVTSFIGQVYVPAMTEMTARIERY
                     NNLAILSSVSMIPVPAYPAPHLPVYNPAPCAPVVPVCTGPVAMSDFEFQDMRAAIASR
                     DFESTRLSMTRQLLSQRNLSTRQVMSLMQLFDFESNRLEVAKFGYSRTIDRNNYYQTF
                     ALFDFDSSVRELSRFMETYS"
     gene            4069..4746
                     /locus_tag="P5292_01585"
     CDS             4069..4746
                     /locus_tag="P5292_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRS37846.1"
                     /translation="MEVQFQIKHNEKLYLRDPELSVIGRKMVRQSIGMINKMGFEQFT
                     FKKLAEAIDTTEASVYRYFENKHKLLLYLLAWYWSWLEYQVVFHTNNLSEPEKKLRKI
                     IDLLTRRPEPMSDASEIDFNALFQIVISEGSKSYLTKDVDQLNKHKLFKPYKDLCARI
                     SDAILEYNSRYRYPHSMATTLLEMSHYQYFFMLHLPSLTDFGDAKTPEGIRNFLESLL
                     FSAIAKK"
     gene            complement(4751..4891)
                     /locus_tag="P5292_01590"
     CDS             complement(4751..4891)
                     /locus_tag="P5292_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37847.1"
                     /translation="MKELESKFGHHPEMLEEKLHALAKALLQQKALEEAWLTLLVSLQ
                     VR"
     gene            complement(4888..5628)
                     /locus_tag="P5292_01595"
     CDS             complement(4888..5628)
                     /locus_tag="P5292_01595"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRS37848.1"
                     /translation="MRTIDPGSVKTSVFHSYMLGAIAPRPIAFASTVDKDGKVNLSPF
                     SFFNAFGSKPPVIVFSPARRVRDNSIKHTLENVYETMEVVINVVNYAMVQQMSLASTE
                     YPKGVDEFVKAGFTPVASELIRPPRVKESPVQMECKVLQVIETGTEGGAANLIVCEIL
                     RMHIDESVMTDDKIDPFKIDLVARMGGDWYCRASGAALFEVPKPNVKLGIGYDQIPEP
                     IRNSKVLSGNDLGQLGNIEALPTASVTS"
     gene            complement(5682..6026)
                     /locus_tag="P5292_01600"
     CDS             complement(5682..6026)
                     /locus_tag="P5292_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37849.1"
                     /translation="MTRIDLHKLKPGEHLTGDRLQRYRSGRMSSEERAAADRHLQSCQ
                     LCSEAVEGIGRLDTPEQLDRIRRDLHRRVAERLRNPRRLFPEHYLPILLITMVVLAII
                     VLLGFYMTTRLH"
     gene            complement(6013..>6246)
                     /locus_tag="P5292_01605"
     CDS             complement(6013..>6246)
                     /locus_tag="P5292_01605"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRS37850.1"
                     /translation="NKEEQLYTELRIEELERAMGMLQVHQRRCIEMFFIQELSYQQIS
                     DQTGFSLKQDKSYIQNGKRNLRIRLGGTGHDED"
BASE COUNT         1484 a         1618 c         1665 g         1479 t
ORIGIN      
        1 gtggttcgcc aaatcttccg gaactacagc cgggaagagc aagttcattc ccgtcagcca
       61 ggaagccctg gatgagtgtc attacaaagg agggaaagac ctactgagta tctattgttc
      121 gaactatcct gatacgctga tttttaccgg caaaggactt accctgggag gaagccatcg
      181 ggctgcgaaa gatggaagcg aagcgtttgc gggtgatatc tccgcgatcc tgatgcagaa
      241 catgccgttc tgggcgcaga tgatccgtac gccggatctt tccatagccc tgatggacga
      301 atgggaagag aagatcgagc ggatggctcg cgccacgatt caggagaacg taacgtccat
      361 cagcggtgtc cccacctgga cagctgtgct ggccaatcgt gttttggaaa ttaccggaaa
      421 gcggaacttg ctggaagtct ggcctaacct agagctcttc attcacggag cggtaagctt
      481 tactccctac cgggaacagt tccgatcact ttgtccggcc gacagctttc actacctcga
      541 gacctacaat gcttcggagg gctttttcgg cattcaggac ctccgcaatt cggaggagtt
      601 gctgttgatg cttgattacg gagtgttcta tgagttcatg ccgatggaag agtacggaaa
      661 ggaatttccg gaaaccgtcg gactcgaggg cgtggagatc ggcaggaact atgcgctggt
      721 catttcaaca aacgcgggat tgtggcgtta tgtcatcgga gacacggtac gttttacatc
      781 cctggatccc ttccggatcc gcattaccgg aaggacacga ttattcatca atgcgttcgg
      841 agaagaggtg atcattgaga acacggatat ggccgtccgc atcgcgtgcg agaaggcgaa
      901 ctgccttgtg cgggagtaca cggtagcgcc ggtctatttt aaccagcggg agaacggtgc
      961 ccatgaatgg ctcatcgagt tcgagcggtc tccagatcac ctggactatt tcaaagagct
     1021 actcgataac gcactcaaat ccctgaactc cgactacgaa gcgaagcgct accgggatat
     1081 ggctttgcgc tttccggtca tccgggaggt tccgcagggg acttttcacg agtggttgcg
     1141 gatgaaaggc aagcttggcg gccaacacaa ggtgccacgg ttgagtaacg agcgccagca
     1201 tgtagaggag attcttcaat tgttgcgcga acgggagcgt ccgatccagg catgaagcga
     1261 cttttggtta tcctgttcct gttcacggct ggtgcattcc ggccggcgga ggattttcac
     1321 ttgttgcatc aattgccggt gagcggaaat gcgaccgtga ccaccgaccc cttgggaaat
     1381 tgttacctga cggtaggcaa ccaggtgttg aagttcgatc cgaaaggcaa gccgctgagt
     1441 aatttcgctc aggtcgatcg gggcgaactg cgaggcgttg acgcgctgaa tcccctgaag
     1501 gtcctgctgt tctatcctga tttcgcccag atcatcctgc tgaatacaca attgtcggtt
     1561 cagtcgacca tcaatttgcg taacctggac atccagcagc cctggctggc ctgctcctct
     1621 ttgaacgatg gctattgggt gtttgatttg caggatgccc aattgaagaa gatcggtctg
     1681 gatctgcaac tccagtttca aagcggcaat ctgaaccagt tgttacgtaa gcgccttgtt
     1741 ccgatggcct tgctggaatc gcaggagagc gttttcctgg ctgaccctga ggcgggaatt
     1801 ctcgtattcg accggtttgg taattacgat aagacacttc cggttccggg ggtgcggaag
     1861 tttcaggtga tcggtgaaga agtactttac ttgcggaacg attccgtctt cgcggtgaac
     1921 cgcaagacgt tggaagaaca tcccgttgta cttccttcac acgaaggcgc tgtccgggat
     1981 ttccggttcg agcaacagga actttattta ttaacaaccg acgcgcttag catctattca
     2041 ttttaatttg ccctctacac acacactgta accggatttt cctcgcatgt catcaaagcc
     2101 tataacactc cggaagtccg cttccaaaaa tcaagcgaaa gtgacctcca ccaaaacctc
     2161 tggttcacgc gcaactgcta ccgaaaaaaa caccgaaaaa tacgcgccga ccaagaagca
     2221 acttgcgccg cttcatctgg cgatcgccgg caacatcgga gcaggtaaaa ccacgttgac
     2281 caatcttctg gcgaaaacca tgaagtggga acctcatttc gaggacgtgg acgataatcc
     2341 ttacctcaac gacttttatg aagacatgca gcgttggtcg ttcaacctgc agatttactt
     2401 cctcaatgcc cggttcgagc aggtgatgaa gatccgtcag agcggcaaga ccgtcattca
     2461 ggaccgcacc atctacgaag acgccaatat cttcgcgcct aacctgcatt cgatgggcct
     2521 gatgaccacc cgcgatttca acaattacca atcgctcttc agcctgatga cgaagttcat
     2581 cagtcctccc gacctgctca tctacctccg tgccagcgtg ccgaccctgg tcaatcagat
     2641 cggcaagcgg ggacgtgatt acgagaactc gatccggatc gattacctca agcgcctgaa
     2701 tgaacgctac gaagcatgga tctccaccta tgagcagggt aagttgctca tcatcgatgt
     2761 cgacgacctg aatttcgccg acaatcctga agacatgggc aagatcgtta acaagatcaa
     2821 cgccgagatc aacggactct tctgatcaac cactcaactc aataggaaaa gccagcccga
     2881 aagctggctt tttcatttaa tgtacgggta caataaaaaa ccgggtgctt acgggcaccc
     2941 ggtttacaca cacaccactc aataacgaac catgggactg gttcaactat acgtttccat
     3001 gaatcggctc aattcccgta cgctgctgtc gaagtcgaag agcgcgaaag tctgatagta
     3061 gttgttgcgg tcgatcgttc gggagtagcc gaacttggcc acttccaggc gattggattc
     3121 gaagtcgaac aattgcatca ggctcatgac ctgccgtgtg ctcagattcc tttgcgacag
     3181 taattgacgg gtcatgctga ggcgggtgct ttcgaaatcg cggctggcga tggctgctcg
     3241 catatcctgg aattcaaagt cgctcatggc taccgggccg gtgcataccg gtacgaccgg
     3301 agcgcaggga gcgggattgt acacgggcag gtggggagca gggtaggccg gtacggggat
     3361 catactgacc gacgacagga tcgcgaggtt gttataacgt tcgatgcgag ccgtcatttc
     3421 cgtcatcgcg ggcacgtaca cctgaccaat gaaactggta acagggtaat gaccatgatg
     3481 gccgtttcct gaccatgtcg ttgcttcgag catatggtag cccggctgta atccggttac
     3541 cgtatagttg cgctctccgg atccgaacgg cacgccgtcg acgcgaagcg cgaaacgttt
     3601 gttgttgtac tggtgcaggt ggatgaccga tcctccgtac tgcgcggctg cactcagcgc
     3661 aaaggtgagg atcaacagag tgtagagggt ctttttcatg ggaattcctc ccgggtttta
     3721 atgtacccgg gctatttcca aatccgtgcc aagccggccg gaaaagaatg aatcctttat
     3781 gaagagggtg gaagatgagc ggaaaccgac cgtttggtca gaaaaaaggc gtggtgcgct
     3841 accggaatag tttccctaac ccattaacac gcatcaccaa aacccgaaat gcgcattgac
     3901 gtacccggta gcgcaccaca cctgcggggg tgtccatttg ttcaaccgaa tggatatcct
     3961 gcaaacttac tgaataatag tattactact attcattaaa gggttattaa tttattggtg
     4021 gtggtagtcg aattctctag ttttgatcga atttcccacc accaccacat ggaagtccag
     4081 tttcagatca agcacaacga gaagttgtat ctccgcgatc cggagttgtc ggtcatcggc
     4141 agaaaaatgg tccggcaaag tatcggcatg atcaacaaaa tgggtttcga gcagttcacg
     4201 ttcaagaaac tcgccgaagc gatcgacact acggaagcca gcgtctatcg ctattttgag
     4261 aacaagcaca agttgctcct gtacctgttg gcgtggtact ggagttggct cgagtaccag
     4321 gtggtgtttc ataccaacaa cctgagcgag cccgaaaaga aattacggaa gatcatcgat
     4381 ctgcttacgc gtcgaccgga acccatgtcc gatgccagtg agatcgactt caacgccttg
     4441 tttcaaatcg tcatttcaga aggcagtaaa tcgtatctga ccaaagacgt cgatcaactg
     4501 aacaagcaca agttgttcaa accgtacaag gatttgtgtg cacgaatttc cgacgcgatc
     4561 ctggaatata attcccggta tcgttacccg cactctatgg cgaccacctt actggagatg
     4621 tcccattacc agtatttttt catgctgcac ctgccgtcgt tgactgattt cggtgacgcg
     4681 aaaacgccgg aaggaatacg gaactttctg gagtcgctct tgttttctgc gatcgctaaa
     4741 aagtgactgc tcagcgtacc tgaagcgaaa ccaacaaggt caaccaggct tcttccaggg
     4801 ctttctgttg cagcagggcc tttgccaacg cgtgtagttt ctcttccagc atttccggat
     4861 ggtgaccgaa tttcgattcg agttctttca tgaagtcacg ctggcggtag gcagcgcttc
     4921 gatattgccg agttgtccca ggtcgtttcc actcagcact ttgctgttgc gaatgggttc
     4981 gggaatctga tcgtagccga tgcccagctt cacattcggt ttcggaacct cgaacagcgc
     5041 ggctcccgac gcgcggcagt accagtcacc gcccatacgg gcaaccaggt cgatcttgaa
     5101 cgggtcgatc ttgtcgtcgg tcatcacgga ttcatcgatg tgcatgcgaa ggatttcgca
     5161 aacgatcagg ttcgcggcac cgccttccgt tccggtttcg atgacctgaa ggaccttgca
     5221 ttccatttgc accggtgatt cctttacccg cggaggacgg atcagttctg atgccaccgg
     5281 cgtgaacccg gccttgacga attcatcgac accttttgga tactccgtgg aagcgagcga
     5341 catctgctgg accatggcat agttgaccac gttgatcacc acttccatgg tctcataaac
     5401 attctcgagt gtatgcttga tgctgttgtc gcgcacgcgt cgggcagggg agaagacgat
     5461 aaccggcggc ttactgccaa atgcgttaaa aaaagagaag ggagaaaggt tcacctttcc
     5521 gtctttatcg accgtgctcg cgaacgcgat gggacgcgga gcgatggcgc ccagcatata
     5581 actgtggaat acactggtct tgacggaacc gggatcaatg gtacgcatgc agctgaatta
     5641 ttcaccgcaa aggtaacgcc cttcgggcat tcggcgaagc atcagtgaag gcgagtcgtc
     5701 atgtagaatc cgagcaagac gatgatcgcc aggacgacca tggtgatcag gagaatcggc
     5761 aggtagtgtt ccggaaagag gcgcctcgga ttgcgcaatc gctcggcgac cctgcggtgc
     5821 aggtcgcgcc ggatgcggtc caattgttct ggcgtatcga gcctgccgat gccttccacc
     5881 gcttcactgc agagctgaca cgactgcaag tgacgatcgg ctgcagcacg ctcttcggaa
     5941 ctcatacgtc cggagcgata gcgctgcaag cggtcaccgg tcaggtgttc gccaggctta
     6001 agtttgtgca ggtcaatcct cgtcatggcc agtacctccc agacggattc tgagatttcg
     6061 tttaccgttc tgaatatagc ttttatcctg tttcagcgaa aagcccgttt gatcgctgat
     6121 ctgctggtag cttaactcct gtatgaagaa catttcgatg cacctgcgtt gatgcacctg
     6181 gagcatgccc atggcgcgct ccaattcttc gatcctgagt tcggtgtaga gttgctcttc
     6241 cttgtt
//
LOCUS       DAOTON010000045         6208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_10834_length_6208_cov_4.084349, whole genome shotgun sequence.
ACCESSION   DAOTON010000045 DAOTON010000000
VERSION     DAOTON010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10834_length_6208_cov_4.084349"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="P5292_01610"
     CDS             <1..814
                     /locus_tag="P5292_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRS37851.1"
                     /translation="APNGWPGIQSGAAIVFFAFIGFDAVSTAAQEAKNPKRDMPIGIL
                     GSLVVCTILYILFGHVLTGVANWQDFVDPEKGREASVAYAISAYMPGYHWLATAVTVA
                     ILAGFSSVILVMLMGQSRVFFTMSNDGLIPSVFGEVHPRYKTPYKANAILFFFVGAFA
                     AFVPGHVAGDLTSFGTLFAFVLVSLGVWILRVKAPNIERPFRTPVAPVVSLLGALICT
                     AMIIGLDSQTLKVAFGWMAVGLVIYFLYSRKRSKLRQFGDVMPKASDFEQKG"
     gene            941..2113
                     /locus_tag="P5292_01615"
     CDS             941..2113
                     /locus_tag="P5292_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HRS37852.1"
                     /translation="MTIFLESPSFVRDIWLLLISLLLFGATLYIYVRLRSNTFRRIRH
                     LLEERVEVKTRQLVEKNLELEQLSLVASRTDNSVLIAAPDTRIEWVNDAFLRLYAMPK
                     DKVLGMRLHEIAIYRDIDQQVEQTLKQEHSSIFESNVTTHHPRNIWISSTLTPVYDEE
                     ARLKKLVMVDTDITSTKQLQQQIEASLKEKDVLLKEIHHRVKNNLQIIISLLNLQSGY
                     IKDESTLKAVQDGQNRVRSMALVHEKFYQADELTEIAFGEYMEKLCQYLYQSYGDKTD
                     RVRVLIDACDVALDMDTAMPCGLLVNEIVSNSYKYGFPGQRSGEIRIRMWREDKTVTL
                     DITDNGIGLPEGFSVESSESLGMQLIQALTSQLDGELSIGRENGTRFTVKFPYPKG"
     gene            2189..3331
                     /locus_tag="P5292_01620"
     CDS             2189..3331
                     /locus_tag="P5292_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS37853.1"
                     /translation="MSALKIMIVEDEIIVAKDIQRILKKLGYEAFDPFANGKKALDAV
                     EKLNPDLILLDINLKSSELDGIQVGEQIHQHYQIPFIYLTAFSDKNTLDRAKLTEPYG
                     YIIKPFEEDDIRTAIEIAYYKYTKDLELQNKGNRFAAALDTLDVAVIITDANEKVIFM
                     NKMAETLTGCLKQEALGKDISVAMKNSGSETKSVFKTLAHTVVGESQKQDGDSAVTIS
                     NGAAENKVAVNTFPILTVNNKINGCAFVISGGGSPRREAGTETTTDKNLFNFLENIYA
                     NNSFFVKKDSRFVRVNFQDILWIEALDNYVIIKTSSKEQFILHSSMKDIEAKLPPLNF
                     ARVHRSYIVQLEKIGALEENACIIDGNRIPIGKSYKENLMSRLNLF"
     gene            3447..4208
                     /locus_tag="P5292_01625"
     CDS             3447..4208
                     /locus_tag="P5292_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS37854.1"
                     /translation="MKQPIIVVDQLVKHYPGVQAVNGISFEVHEGEIFGLLGPNGAGK
                     TTTLEIIETLREKTAGSVRVCGYDVDRDVNEIKQRIGVQLQAAGYYPSLTLIELLRLF
                     SGLYNVTIDPMEMLRRVGLEDKGKAKYKELSGGQKQRFSICTTLINRPQVVFLDEPTT
                     GLDPQARRNLWELIREIRQQGTTVVITTHYMDEAENLCDRVAIVEKGRIIALDTPDRL
                     IDQLVASGFERPREVKKANLEDVFLSLTGHSWRDQ"
     gene            4210..5355
                     /locus_tag="P5292_01630"
     CDS             4210..5355
                     /locus_tag="P5292_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS37855.1"
                     /translation="MNAPQQPRYNQLKAMLAISKASLRSISRSPSAVIFSIGFPLVFI
                     VVFGFIRGNSIRLDVGVAPTCDTTNVIYRTVAQLPNIRLKSGLPQEQLDDQLIKGRID
                     AVLNIHPSDTVRVVEVITSLASREKGMFVKSVLTNLIDKGNLAHFRSAGSVFDSLIVV
                     RNGEHEPLAELRESEVNGREYKMIDFVLPGQLGFSILSAGVFGTAFVFFSLRQTLVLK
                     RFFATPIRRTYIVLGEALSRLVFQLAGAMVIIVLGKYAFGFTLINGWTTFLTMLLLSA
                     IGLVVFMGFGFVVSGLARNESTIPPIANIITLPQFLLSGTFFPVDAFPGWLQKIANVL
                     PLTWLNDALRKIAFEGAGFGDISTHLLVILAWGIVVYAAAVRVFRWE"
     gene            5427..>6208
                     /locus_tag="P5292_01635"
     CDS             5427..>6208
                     /locus_tag="P5292_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37856.1"
                     /translation="MSPLWRYSYRSIVLLLALFLPGNLLAQLGKDGAKTVTAAGTIVN
                     EYTTLTADALAGTSMLTVTSSSLNANGRFPAALAPGDLVFIIQIQGASMTSPDDSTYG
                     TIISMGNCGRQELAEVAAVPTATSIQLSCALQNSYTWVGRAQVVRVPRYASITINSGG
                     TLTCPNWNGSTGGVLALEVQGNTVINSGGRIDVTGLGFRGGQLLDNNSWYGVGNHRWM
                     TDDYGAEKGEGVLGYQVDYDMFGGRYCRGAAINAGGGGNGHNA"
BASE COUNT         1341 a         1803 c         1765 g         1299 t
ORIGIN      
        1 cgcgccgaac ggctggccgg ggattcaatc cggcgcggcg atcgtcttct ttgctttcat
       61 cggcttcgac gcggtgagta ctgccgcgca ggaggccaag aaccccaagc gcgacatgcc
      121 gatcgggatc ctgggttccc tcgtcgtttg taccattctg tacatcctct tcggacacgt
      181 gctcaccggc gtagccaact ggcaggactt tgtcgatccc gaaaaggggc gcgaagcatc
      241 cgtcgcctat gcgatctccg cttacatgcc gggctatcac tggctggcaa cggcggtgac
      301 ggtcgccatt ctggccggct tctcttcggt tattcttgtg atgctcatgg gacagagccg
      361 cgtgttcttc acgatgagca acgacggcct tattccgtcg gtcttcggcg aagtacaccc
      421 gcgctacaag acgccctaca aagccaacgc gatcctgttc ttcttcgtgg gcgccttcgc
      481 ggccttcgtt ccgggacacg ttgccggcga cctgaccagc ttcggcaccc tcttcgcgtt
      541 cgtgttggtg agcctgggcg tctggatcct ccgcgtgaag gcgccgaaca tcgaacgccc
      601 gttccgcacg ccggttgcgc cggtcgtgtc cctgctcggc gcgctgatct gtacggccat
      661 gatcatcggc ctcgactcgc aaacgctgaa agtggccttc ggctggatgg cggtcggctt
      721 ggtcatctac ttcctctata gccgcaagcg cagcaagctg cggcagttcg gcgacgtcat
      781 gcccaaggca tccgacttcg aacagaaggg ctgatcgatc ttattctcct gacaacgggc
      841 ccgttcggaa gagcgggcct gttctttttg tgttgggata acatttttcg ttcgcagtag
      901 tataaacggg cggaaagcta tgcctgcgcc ctttcgtttg ttgaccattt ttctggagag
      961 cccgtcgttc gtgcgcgaca tctggctgtt gctgatcagc ctgttgctgt tcggcgcgac
     1021 gctgtacatc tatgtgcgcc tccgcagcaa cacctttcgt cgtatccggc atttgctcga
     1081 agaacgcgtc gaagtgaaga cccggcagct tgtcgagaag aacctggaac tggaacagct
     1141 ttcgctcgtg gcgagccgta ccgacaacag cgtgctcatc gccgcgcccg acacccgcat
     1201 tgaatgggtg aacgacgcct tcctccggct ctacgcgatg cccaaagaca aggtgctggg
     1261 catgcgcctg cacgagatcg ccatttaccg cgacatcgac cagcaggtgg agcaaacgct
     1321 gaagcaggaa cactcgagca ttttcgaatc caacgtcacc acccaccatc cgcggaacat
     1381 ctggatcagc agtacgctga caccggtgta cgacgaagag gcgcggctga aaaaactggt
     1441 gatggtggac accgacatca cttccacgaa gcaactgcag cagcagatcg aagcctcgct
     1501 gaaggagaag gacgtgttgt tgaaagagat ccaccaccgc gtcaagaaca acctgcagat
     1561 catcatcagc ctgttgaacc tgcagtcggg ttacatcaag gacgaatcga cgcttaaggc
     1621 agtgcaggac ggacagaacc gcgttcgttc gatggcgctg gtgcacgaga agttctacca
     1681 ggccgacgaa cttaccgaga tcgcctttgg cgagtacatg gaaaaacttt gtcagtacct
     1741 gtaccagtcg tacggcgaca agaccgaccg cgtccgcgtg ctgatcgacg cgtgcgacgt
     1801 agcgctcgac atggacacgg cgatgccttg cggattgctg gtgaacgaga tcgtgtcgaa
     1861 cagctacaag tacggcttcc ccggacagcg cagcggcgag atccgcatcc ggatgtggcg
     1921 ggaagacaag accgtaacgc tggatatcac cgacaacggc atcggcctgc cggagggatt
     1981 ttcggtggaa tcctcggaat cgctgggtat gcagctcatc caggcgctga ccagtcagct
     2041 cgacggcgag ctgagcatcg ggcgggagaa cggaacgcgg tttacggtta agttcccgta
     2101 tccaaagggc tgattatttg atttttacgc attttaaggt aacctaatcg ggcggcgtcc
     2161 ggtacaaggt tatgatatac tttgacctat gtctgctttg aaaataatga ttgtcgagga
     2221 cgagatcatc gtcgcgaaag acatccagcg cattttgaaa aagttgggtt acgaagcgtt
     2281 cgatccgttc gccaatggta agaaggcgct cgacgcggtg gagaagttga atccggacct
     2341 catcctgctc gacatcaacc tgaagagcag cgagctggac ggcatccagg tgggcgagca
     2401 gatccaccag cactaccaga tcccgttcat ctacctcaca gccttctccg ataagaacac
     2461 cctcgaccgc gccaagctga cggagccgta cggctacatc atcaagccgt tcgaagaaga
     2521 cgatatccgc accgcgatcg agatcgcgta ttacaaatac accaaggacc tcgagctcca
     2581 gaacaaaggc aaccgtttcg ccgccgcgct ggacacgctc gacgtagccg tgatcatcac
     2641 cgacgccaac gagaaagtca tcttcatgaa caagatggcg gaaacgctga ccggctgtct
     2701 gaagcaggag gcgttgggca aagacatcag cgtcgcgatg aagaactccg gttcggagac
     2761 caaatccgtt ttcaagaccc tggcccatac ggtggtcggc gagtcgcaga agcaggatgg
     2821 cgattcggcc gtgaccatca gcaacggcgc ggcagagaac aaagtcgccg tgaacacctt
     2881 cccgatcctg acggtcaaca acaagatcaa cggctgcgcg tttgtgatca gcggcggagg
     2941 ttcgccccga cgcgaagccg gcacggagac cacgaccgac aagaacctgt tcaacttcct
     3001 ggagaacatt tatgcgaaca acagtttctt cgtcaagaag gactcgcgtt tcgtacgcgt
     3061 gaacttccag gacatcctct ggatcgaagc gctggacaac tacgtcatca tcaagacctc
     3121 gagcaaggag cagttcatcc tgcacagctc gatgaaagac atcgaagcca agttgccgcc
     3181 gctcaacttc gcgcgcgtgc accgttccta catcgtgcag ctcgaaaaga tcggcgcgct
     3241 ggaagagaac gcctgcatca tcgacggcaa ccggattccc atcggcaagt cctacaagga
     3301 gaacctgatg agccgactca acctgttctg atcgcaacag caacccattg aagccgcccc
     3361 cggaaacccc gtggggcggt tttgctttca tgcacccttc attcatccgg tgatcgccgg
     3421 aagggcggaa gccttacctt cgccccgtga aacagccgat cattgtcgtc gaccaactgg
     3481 tgaagcacta tcccggcgta caggcggtga acgggatcag cttcgaggtg cacgaaggcg
     3541 agatcttcgg gctcctcggg cccaacggcg cgggcaagac caccacactg gagatcatcg
     3601 aaacgctgcg cgaaaaaacc gcgggcagtg tacgcgtctg cggctacgac gttgaccgcg
     3661 atgtgaacga gatcaagcag cgcatcggcg tgcagttgca agccgcgggc tattatccga
     3721 gtcttaccct gatcgaactc ctccgcttgt tctccggcct gtacaacgtc accatcgatc
     3781 cgatggagat gctccgacgg gtcggactgg aagacaaggg caaggcgaaa tacaaagaac
     3841 tttccggggg acagaaacag cggttcagca tttgtaccac gctgatcaac cgcccgcagg
     3901 tcgtgtttct ggacgaacca acgaccggac tcgatccgca ggctcgccgc aatttgtggg
     3961 agctcatccg cgagatccgg caacagggca ccaccgtggt gatcaccacg cattacatgg
     4021 acgaggccga gaatctttgc gaccgggtag ccattgtcga gaaaggccgc atcatcgcgc
     4081 tcgatacccc ggaccggctg atcgatcaat tggtggcgag cggcttcgag cgtccgcgcg
     4141 aagtgaagaa agccaatctg gaagacgtat tcctgtcgct caccggacac agctggaggg
     4201 accaataaga tgaacgcccc acaacaaccc cggtacaatc aactgaaggc gatgctggcc
     4261 atcagcaagg ccagcctgcg ttcgatctcg cgcagtcctt cggcggtgat cttcagcatc
     4321 ggatttccgc tggtgttcat cgtcgtgttc ggattcatcc gtggcaactc tatccggctt
     4381 gatgttggtg tagcgcccac ctgcgatacc accaacgtga tctaccgcac ggtggcgcag
     4441 ttgcctaaca tccggctcaa gagcggcctg ccgcaggaac aattggacga tcaattgatc
     4501 aaaggacgca ttgacgcggt attgaatatt cacccaagcg atacggtgcg tgtggtggag
     4561 gtcatcacct cgctcgcctc acgggagaag ggcatgttcg tcaagtcggt gctcaccaac
     4621 ctcatcgaca aaggcaatct ggcgcacttc cgttcggcgg gttcggtttt cgattcgttg
     4681 attgtcgtca gaaacggaga acatgaacca ctggccgagt tgcgggagtc ggaagtgaac
     4741 ggtcgggagt ataagatgat cgacttcgtg ctaccggggc agttgggatt ttccattctc
     4801 agcgccggcg tgttcggaac cgcgttcgta ttcttctcgc tgcgtcagac gctggtgttg
     4861 aaacgctttt tcgcgacccc gattcgtcgt acctacatcg tgttgggcga agccctgagc
     4921 cggctcgtct tccaacttgc cggcgcgatg gtgatcatcg tgctgggtaa atacgccttc
     4981 gggtttaccc tcatcaacgg ctggaccact ttcctgacca tgctgttgtt gtcggcgatc
     5041 ggtttggtgg tgttcatggg cttcggtttc gtcgtctccg gccttgcgcg caacgaaagc
     5101 acgattccgc ccattgccaa catcattacc cttccccagt tcctgcttag cggtacgttc
     5161 ttccctgttg atgcctttcc gggctggtta cagaagatcg cgaatgtctt gccgctgacc
     5221 tggctgaacg acgccctgcg caagattgcc tttgagggag ccggttttgg cgatatttcg
     5281 acccacctgc tggtcatatt agcctgggga atcgtcgtgt atgccgccgc ggtacgggtt
     5341 ttccgttggg aataggacaa aacggtgtca cttttccttc gcctttacgt atcttggtcg
     5401 aaggtaaaac cctctcccgc agtatcatgt cccccctttg gcgttactct taccggagta
     5461 tcgtattgct tcttgccctt tttcttccgg gcaatctttt agcccaactg gggaaggacg
     5521 gcgccaaaac cgtcacggca gccggcacca tcgtcaacga gtacaccaca ctgaccgccg
     5581 acgccctggc cggaacctcc atgctgacgg ttaccagcag ttcgttgaat gcgaacggac
     5641 gatttcctgc agcacttgca ccgggcgatc ttgttttcat catccagatc cagggcgcga
     5701 gcatgacctc gcccgacgat tccacctacg gaacaatcat ctccatgggc aactgtggaa
     5761 ggcaggagct cgcggaagtt gcggctgttc cgaccgctac atcgattcag ctttcctgcg
     5821 ccctgcagaa ctcgtacacc tgggttggtc gtgcgcaagt ggtgcgcgtg ccgcgctatg
     5881 cttcgattac gatcaacagc ggcggcactc ttacctgtcc gaattggaac ggaagcaccg
     5941 gaggcgtgct ggcactggaa gtacagggca acacggtcat caactccggt ggacgaatcg
     6001 atgttaccgg tcttggtttt cgcggaggcc agttgctgga taacaacagt tggtacggcg
     6061 tcgggaacca tcgttggatg accgacgatt atggtgccga gaaaggcgaa ggcgtcctgg
     6121 gttaccaggt cgattacgat atgttcggag gacgttactg tcgtggcgcg gcgatcaacg
     6181 ccggtggcgg cgggaacgga cacaatgc
//
LOCUS       DAOTON010000046         6179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_10891_length_6179_cov_3.546048, whole genome shotgun sequence.
ACCESSION   DAOTON010000046 DAOTON010000000
VERSION     DAOTON010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10891_length_6179_cov_3.546048"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..740
                     /locus_tag="P5292_01640"
     CDS             198..740
                     /locus_tag="P5292_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015361082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HRS37857.1"
                     /translation="MTETSRQYDAVVARCKDIFLKKMKDYGTAWRILRVPSITDQIFI
                     KAQRIRSIEEKGTQRVDDGIDQEFIGIINYAVIALIQLELGVNDETNMLEQDVVKRFD
                     EQVRTAKRLMEDKNHDYGEAWRGMRTSSMTDLILMKLLRIKQIEDNRGQTLISEGIAA
                     NYHDIINYAVFAMIKLAEPA"
     gene            789..1907
                     /locus_tag="P5292_01645"
     CDS             789..1907
                     /locus_tag="P5292_01645"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRS37858.1"
                     /translation="MKVLAQISRVFVGILFIISGFIKANDPLGFSYKLDEYFQVFHMP
                     WLSAVSLALAIGICAFEIGLGVALLLGAKMRFTAWSLLLMIVFFTFLTFYSWKFDVVK
                     DCGCFGDALHLKPFESFMKDVILFVLILFIFIKRNEIKPLFGESGSTWLAYAGFVASL
                     TFSIYCYRHLPMIDFRPYAIGKSIPEGMKLPPNAVTDSVVMVFIYEKDGKRMELSMDQ
                     ISGIDSTYKFVDRTDKVIREGDKAPIHDFSIVSADGTDVTADVLSMDRVFLLVAYDLE
                     KSDEDAQSRINDFVALSQKAGVEFIGLTSSAPATVDAFKKKHHSAFEYYFTDGTTLKT
                     MIRSNPGLLLLKKGTVAAMWHHNDLPAFDEVNSMYLSK"
     gene            complement(1923..2636)
                     /locus_tag="P5292_01650"
     CDS             complement(1923..2636)
                     /locus_tag="P5292_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRS37859.1"
                     /translation="MSSTERTGLPEILYEDNHLIAVNKRPGDIVQGDKTGDAPLGDLL
                     KAYIKEKYKKPGEVFLGVVHRLDRPVSGVVVFARTSKALARMNELFRERRTQKTYWAV
                     VRTAPPQASGSLIHYLKKDEKNNKSRAYAQEVPGSSRAWLDYRLLGKSDHYYLLEVLP
                     HTGRHHQIRVQLSTMGCPIKGDVKYGAERTNENGSIHLHARRLVFLHPVKKEEIVLTA
                     QPPDDAVWNYFSRSLSEES"
     gene            complement(2623..3444)
                     /gene="panB"
                     /locus_tag="P5292_01655"
     CDS             complement(2623..3444)
                     /gene="panB"
                     /locus_tag="P5292_01655"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRS37860.1"
                     /translation="MSSAQSSEEKKITTHVLQEMKRRGEKISMLTAYDYSMARIIDAA
                     GIDVVLVGDSASNVMAGYETTLPITLNEMIYHAASVVRAVSRALVVVDLPFGSYQGNS
                     KEALNSSIRIMKESGAHAVKLEGGREVKESIERILTAGVPVMGHLGLTPQSIYKFGTY
                     VVRAKEDTEAQRLIEDAHILAESGCFAIVLEKIPAQLAARVAAEVKIPIIGIGAGAGV
                     DGQVLVLHDMLGITHEFSPRFLRRYMNLYDDIKAATERYISDVKSKDFPNKHEQY"
     gene            complement(3457..4290)
                     /locus_tag="P5292_01660"
     CDS             complement(3457..4290)
                     /locus_tag="P5292_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37861.1"
                     /translation="MRYRLFYMAMTVTALTYRFTIADVNGQKVRLDFPKTFFRDDFVS
                     DSGHWKELSNASNLLLLQKGEYILQRKSSGSYSSFPSLKNIQAPFSLTASLRIDDGTD
                     PESGIGLIFMAQPSGNGGFTLELNARKEYRIRQLVGVSYRLLTGDNRNLGWTKCEWLN
                     GKSTWNTIQVRTADRNYDVYINGQFVTSFSEPQYFGGDFGIAIGPNSSGRVDWIDVRT
                     PDQSEEPIIVLPVAADDPIRLQEELAHLKKENQALRDSLQRCRQAKPVIKKPLNNGHQ
                     E"
     gene            4347..5345
                     /locus_tag="P5292_01665"
     CDS             4347..5345
                     /locus_tag="P5292_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HRS37862.1"
                     /translation="MKKSLLFLFAALTLASCSEEIDLTADYKETMVLYGLLDPNATNQ
                     YVRISKAFLGEGNTLQMAQQSDSINYANVLDVRLERLSNGVVVNTYPMTRIDTIPKEN
                     GVFAAPYQVIYTTNQRILTDGSLYRIVARNTETGVEASSTTRIVGSMQVFNPSPTVPV
                     VDFSAFGETRPRIKGGDNGRLFGLVMRFRYIEIDSLGAVTRHTADWLFPDQLATQTGE
                     QVEFVYQRQDFYRIIGSQIPVKAGVTRKLDTLTVGLGAIEFRFYSATEDVYTYQQLVN
                     PANTGVVQDRPSFTTVENGLGLFSSRLLQQEYRNLTANSLAAFDTSAYTRDLNFSF"
     gene            5485..>6179
                     /locus_tag="P5292_01670"
     CDS             5485..>6179
                     /locus_tag="P5292_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRS37863.1"
                     /translation="MRKVIGIDLGTTNSCVAVMEGNEPIVIPNSEGRRTTPSVVAFLD
                     NGERKVGDPAKRQAITNPRNTVYSIKRFMGETFDRTTKEKERVPYEVVKGDNNTPRVK
                     ITDRLYTPQEISAIVLQKMKKTAEDYLGYEVSEAVITVPAYFNDAQRQATKEAGEIAG
                     LKVLRIINEPTAASLAYGLDKQAKDEKIAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDQKII"
BASE COUNT         1393 a         1635 c         1644 g         1507 t
ORIGIN      
        1 gtgtcaggtt cacaatcccc atgaccaccg gtgttgataa ttctaaaagc cttccggaga
       61 cgtttaaccg gtacttcatc gtttaagcct tatcttcgta ttcacgagtt agttataaat
      121 tattgataat caatacattg acgaaggttg agtctttagt ccccaactca gttcaccgaa
      181 ctaccggaaa gccgatgatc acagaaacct cccggcagta cgacgcggtc gttgctcgtt
      241 gcaaagatat ttttctgaag aagatgaaag actacgggac cgcctggcgg atcctgcggg
      301 ttccatccat cacggaccag atcttcatca aggcacaacg catccgcagt atcgaggaaa
      361 aaggtacgca acgggtcgat gacggcattg accaggagtt catcggtatc atcaattacg
      421 cggtcatcgc gctgatccaa ttggaactgg gggtcaatga tgagaccaac atgcttgaac
      481 aggatgtggt gaagcggttc gatgaacagg tacggacagc caagcggctg atggaagaca
      541 agaaccacga ctacggcgaa gcctggcggg ggatgcgaac cagttccatg accgacctga
      601 ttttgatgaa gctcttgcgc atcaagcaga tcgaagacaa tcgtggacaa accctcatct
      661 cggaaggcat cgccgcgaat taccacgaca tcatcaacta cgccgtgttc gccatgatca
      721 aactggcgga gccggcctga tcactgttgc atatccattc atcgattcaa ctatagcaat
      781 caccgatcat gaaagtactg gcccagatct cccgcgtctt cgtcggcatt cttttcatca
      841 tctccgggtt cattaaggcg aatgacccct tgggcttctc ctacaagctc gacgagtact
      901 tccaggtctt tcatatgccc tggctgagcg cggtttcgct ggcgttggcc atcggtattt
      961 gcgcgtttga gatcggtctg ggtgtcgcgt tgctgctagg cgcgaaaatg cgattcaccg
     1021 catggtcgct gctgctcatg atcgtcttct ttactttcct gacgttctat tcctggaagt
     1081 tcgacgtcgt caaggactgc ggttgtttcg gtgacgcatt gcacctcaag ccgttcgagt
     1141 cgttcatgaa ggatgtcatt ctcttcgtct tgattctgtt catcttcatc aaacggaatg
     1201 aaatcaagcc cttgtttggt gagagtggtt ctacctggct ggcatatgcc ggctttgttg
     1261 cttccctcac tttttccatc tactgttacc ggcacctgcc catgatcgat ttccgtcctt
     1321 acgcgatcgg gaagagcatt ccggaaggca tgaaactacc gcccaacgca gtaaccgata
     1381 gcgtggtcat ggtcttcatt tatgagaaag acgggaaaag gatggaactg tccatggatc
     1441 agatcagcgg aatcgacagc acctacaagt ttgttgaccg gaccgacaag gtcatccgcg
     1501 aaggtgacaa ggcgccgatc catgatttct ccattgtttc agcggatgga accgacgtga
     1561 cagcggatgt gctgagcatg gaccgtgtct tcctgctggt cgcatatgac ctggagaaat
     1621 ccgatgaaga cgctcagagc aggatcaacg attttgtcgc cttgtctcag aaagcaggcg
     1681 tggagttcat cgggctcacg tccagcgcac ctgctacggt tgatgccttc aagaaaaagc
     1741 accattcggc tttcgaatat tatttcacgg atggaactac cctgaagacc atgatacgtt
     1801 cgaaccccgg cctactatta ttgaaaaagg gtacagttgc ggccatgtgg catcacaacg
     1861 atttgccggc attcgacgag gtcaattcga tgtatctgtc aaaataattc aggcagccac
     1921 gttcagcttt cttcgctcag cgacctgctg aaataattcc agacagcatc gtcgggcggc
     1981 tgagcggtga ggacgatctc ttcctttttt accgggtgca ggaataccaa ccggcgggca
     2041 tggagatgga tcgagccgtt ctcattggtt cgctcagcgc cgtacttgac gtcacctttt
     2101 atcggacatc ccatggttga aagctggact cggatctgat ggtgccttcc ggtatgcggt
     2161 aaaacttcca gcaagtaata gtggtcggat tttccgagta gccggtaatc cagccaggct
     2221 cgcgacgaac ccggaacctc ttgcgcgtag gcgcggctct tgttgttttt ctcgtctttc
     2281 ttcagataat ggatcaagga gccggatgct tgcggtggtg cggtacgaac caccgcccag
     2341 taggtttttt gggtacggcg ttcccggaag agttcattca tcctggccag ggccttggag
     2401 gtgcgagcaa aaaccacaac accactcacc ggcctgtcca gccgatgcac aacccccagg
     2461 aagacctctc cgggcttctt gtatttctcc ttgatgtagg ctttgagcag atcgcccaac
     2521 ggcgcgtcgc cggtcttgtc gccttgtaca atgtcgccgg ggcgtttgtt gaccgcgatc
     2581 aggtgattgt cttcgtagag gatctccgga agtcctgtac gctcagtact gctcatgctt
     2641 gttcgggaaa tccttcgact ttacatcgct gatgtagcgc tcggtggctg ctttgatatc
     2701 gtcgtacagg ttcatgtatc ggcggaggaa tcgcggactg aactcgtggg tgatacccag
     2761 catatcgtgc aagaccagga cctgtccgtc cacaccggct ccggcaccga taccgatgat
     2821 cggaattttc acctcggctg ccacacgtgc cgcgagttga gcaggtatct tttcaagcac
     2881 gatggcaaag cagcccgatt cggccaggat gtgcgcgtct tcgatgagcc gttgagcctc
     2941 ggtatcttcc ttggcccgca cgacgtaggt gccgaatttg taaatggact gtggggtcag
     3001 tcccaggtgg cccatcacgg gcacacccgc cgtcaggatg cgttcgatgc tttctttcac
     3061 ttcacgaccg ccttccagct tcacggcatg tgcgccggat tccttcatga tgcggatcga
     3121 agagttgagg gcttccttcg aatttccctg ataggatccg aaggggaggt cgacgactac
     3181 caaagcccgg ctgacggcac gaaccaccga ggccgcgtga tagatcatct cgttgagcgt
     3241 gatgggcagg gtggtctcgt agcctgccat gacgttcgag gcggaatcgc ctaccagcac
     3301 cacatcgatc ccggctgcgt cgatgatgcg cgccatggaa tagtcgtagg cggtcagcat
     3361 ggagattttt tctccccgcc gcttcatttc ctgcaggacg tgtgtagtga tctttttttc
     3421 ttcggaagat tgtgctgaag acatgggggg agggtgttat tcctggtgac cgttgttgag
     3481 cggcttcttg atgaccggtt tagcttgtcg gcaacgctga agggagtcgc gcagcgcctg
     3541 gttctccttt ttcaggtgtg ccagctcttc ctgcagtcgg attgggtcgt cggcggccac
     3601 tggtaagacg atgatcggtt cttcgctctg gtccggtgtg cgcacatcga tccagtcgac
     3661 tcgaccgctg gagttcggcc cgatcgctat tccgaaatcc ccgccgaagt attgcggttc
     3721 ggaaaacgag gtaacgaact gaccgttgat atagacatca taattgcggt ctgccgtgcg
     3781 tacctgaatg gtattccagg tgctcttccc gtttagccat tcgcactttg tccatcccag
     3841 gttgcggtta tcgcccgtta acaaccgata gctgactccg accagttgtc ggatgcggta
     3901 ttcctttcgt gcgttcaatt cgagggtgaa tccgccgtta ccggaaggct gtgccataaa
     3961 gatcagtccg atacccgact ccggatcggt accgtcatcg atccgcagcg atgcggtcag
     4021 gctgaacgga gcctggatgt ttttaagcga tgggaaagaa gagtaggagc cactcgactt
     4081 ccgctgaagg atgtactcgc ctttttggag caacagtaaa ttgctggcat tcgacaattc
     4141 cttccagtgg ccgctatccg agacaaaatc atcgcggaag aaggtcttgg gaaaatccag
     4201 acggaccttt tggccattaa catccgcgat cgtaaagcgg tatgttaatg cggtaaccgt
     4261 catggccatg taaaagagcc ggtagcgcat gctacaaacc tacgaattca tttcctgttc
     4321 catgaaaaac ggtagttttg cgaagaatga aaaagtctct cctcttcctt ttcgctgccc
     4381 tgacactggc ctcttgttcc gaggaaatcg acctgaccgc cgactacaag gaaaccatgg
     4441 tactctacgg cttattggat cccaacgcga ccaatcaata cgttcggatt tccaaggcct
     4501 tcctgggcga agggaataca cttcaaatgg cccagcagtc cgattccatc aactatgcca
     4561 atgtgctgga tgttcgattg gaacggttat ccaatggcgt ggtggtcaat acctatccga
     4621 tgacgcgtat tgatacgatc ccgaaggaga acggggtctt cgcggcaccc tatcaggtga
     4681 tctatacgac caaccagcgg atactgaccg atggttcgct ttatcgcatc gttgcgcgca
     4741 atacggaaac cggcgtggaa gccagctcga ccacacggat cgttggcagc atgcaggtgt
     4801 tcaatccatc tcccactgtc ccggtagttg atttctccgc ttttggtgaa acaaggccgc
     4861 gcatcaaggg tggcgacaat gggcgattgt tcggcctcgt catgcgcttt cgttatatcg
     4921 aaatagattc gttgggcgca gtcacccgtc atacggcaga ctggttgttc ccggaccaat
     4981 tggcaaccca aacgggtgag caggtggaat tcgtttacca gcgtcaggac ttttaccgga
     5041 tcataggctc gcagataccg gtcaaggccg gcgtcacccg taaactcgat accctgacgg
     5101 tagggcttgg tgccattgag ttccgttttt attccgccac ggaagacgtc tatacctacc
     5161 agcaattggt taatccggcc aatacgggcg ttgttcagga ccggccgagt tttacgacgg
     5221 ttgagaacgg cctgggcttg ttcagcagcc gtttgttaca gcaggagtac cgtaacctga
     5281 cggccaattc cctcgcggca ttcgatacaa gtgcctatac gcgcgatttg aatttcagct
     5341 tttaatgccg ctttgtcagg tcatttttga cctaaaaagc gccatttatt ccagttctgc
     5401 cactttttcg ccagattttt gactggcact atgattgact ttcggggagc gcattacgca
     5461 ccacatcctg aaaatccaat catcatgaga aaagtaatag gcattgacct tggtaccacc
     5521 aactcctgcg tggctgtcat ggaaggcaac gagccgatcg taattcctaa cagcgaaggg
     5581 cgccggacca ccccgtctgt cgtcgcattc ctcgacaatg gcgagcgtaa ggtcggtgat
     5641 cccgccaaac gtcaggccat caccaatccg cgcaacaccg tttattcgat caaacgtttc
     5701 atgggagaga cctttgaccg gactaccaaa gagaaggaac gtgttcccta tgaagtcgta
     5761 aagggcgata acaatacccc gcgcgtcaag atcacggacc gactgtatac accgcaggag
     5821 atctcggcga tcgtactgca gaaaatgaaa aagacggcgg aggactacct cggttacgaa
     5881 gtgtccgaag cggtcatcac ggtacccgcg tatttcaacg atgcgcaacg tcaggcaacc
     5941 aaagaggccg gtgagatcgc cggattgaag gtgctccgca tcatcaacga gccgaccgcg
     6001 gcttctctgg cttatggcct cgacaagcag gcgaaggatg aaaagatcgc cgtcttcgat
     6061 ttgggcggtg gaacgttcga tatctcgatc ctcgaactgg gtgacggtgt gttcgaagtg
     6121 aaatccacca atggcgatac ccacctggga ggcgacgatt tcgatcagaa gatcatcga
//
LOCUS       DAOTON010000047         6164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_10921_length_6164_cov_3.881977, whole genome shotgun sequence.
ACCESSION   DAOTON010000047 DAOTON010000000
VERSION     DAOTON010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10921_length_6164_cov_3.881977"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="P5292_01675"
     CDS             <1..632
                     /locus_tag="P5292_01675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS37864.1"
                     /translation="LALVHKFSGKRDTIKLLDIGCGTGEFLASAKASGMQTKGIEPSA
                     AAREHAIHNHQLNVEGEEGLEKLTESYDVITLWHVLEHVHDLRVRVQKIAEILNNGGV
                     AIIAVPNPNSTDRNTYNKEWAAWDVPRHLYHFTPTTIKRLFNDFSLKHEASIPMKYDA
                     YYVSMLSERYRNNGKNNLPAAFLNGWKSNIHAGSNAELYSSVIYIFRKS"
     gene            complement(636..1763)
                     /locus_tag="P5292_01680"
     CDS             complement(636..1763)
                     /locus_tag="P5292_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS37865.1"
                     /translation="MQATVSPSRPDTATQVRPVKLLIVDDRPENILSLESILEGPGRE
                     ILKAESGNEALRIALKEDIAVMLLDVQMPEMDGFEVARLLKENSKTRDISIIFVTALS
                     KEERYTMQGYEEGAVDYLHKPLDVNVVKAKVAVFEKLYRQKIELRENAERLESVNKQL
                     GEFVYIVSHDLKAPLRGLASLASFMEEELGAEPKVEVVELLNMMKSRTARMQQLIDGI
                     LHYSRMANNRGDREDVDLNELINSIIDLIAPASNVVIETPDNLPVILAERVKLHEVLQ
                     NLIINGIKYNDKAEIRIKIAFEDNGSHYRFHVTDNGVGIRPEHQEKIFGIFQTLQPKD
                     QCESTGIGLTIVKKIIEQQEGAVTIASEVGSGTTFSFTWKK"
     gene            complement(1841..2695)
                     /locus_tag="P5292_01685"
     CDS             complement(1841..2695)
                     /locus_tag="P5292_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRS37866.1"
                     /translation="MAVQAISESIVLTDERVDQVLSVLHSRTGMNFIHYARPSIVRRL
                     RVFLQREGLPDLLALPLLLEHDPALTTRLVNTLTVHFTELFRDPEFFRAIREQVLPFL
                     ATYPRINIWHAGCSTGEEVYSMAIMLDEVNLLRKARIYATDINTDVLEQAQEGSFSPA
                     LFSHYTRNYRQAGGRGQLTDYCAPVNGRIVMKDQLRRRISFHQHNLAADASFNEFHLI
                     LCRNVMIYFDPLLQERATRLFHESLVNLGYFAIGNKESLRFNRMRTSFVTIDGSHKIF
                     RKVRFQKL"
     gene            complement(2677..>6164)
                     /locus_tag="P5292_01690"
     CDS             complement(2677..>6164)
                     /locus_tag="P5292_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS37867.1"
                     /translation="KERLKSLQNIEYPDLKGNILALASTWEEPGMVDSVNAIFKNFEQ
                     VLVYQRQVMNLLVSFDDYEDPMKHFAAEEVVDNEILPRTNGIINSLNKVIVQKRALAE
                     VEHAGMMASSRALMWSVLGIAILIVIVVLMAAFYMSNNIIVPTMKLRNYVLQMGKGEI
                     PEVNLRPRKNAVGQMSEAVIKLAESLRKTTGFAHAIGEGKFEEQFQPMSDKDELGNAL
                     VQMRDSLKTADEDNRQRHWTSAGIAQVNRTLREHSEDIHRLSDELVSSIVGHLEAYHG
                     ALYLVERDDLRDVSWIQLQGSYALDGRAKAKPQLELGEGLVGQCIKDKKSIWLKDAPV
                     HFARIQSGLGDAHASHVIIVPLSYHGQVFGAVEVASFRAFDRNTVGFLEGIGELIASS
                     IASVQANMLTRKLLEETRKQAERLTAQEEELRQTNEELSNQSRLLQASEEELKQSNLE
                     LKENAHALEHQNEILEQAREALSLKARELEQNSKYKSEFLANMSHELRTPLNSVLILA
                     KLLSENKDTNLTDKQIEYARVIHKSGSDLLVLINDILDLSKIEAGKVELLLESTELRI
                     IRDDLRSLFIEVANEKKIEFELEQETNLPEKIITDRVRLEQVIKNMLSNAFKFTPQGG
                     KVSLRMRRPDRNVRFTNPALMKSREVIEFSVTDTGIGIPQEKQQLIFEAFQQVDGSTS
                     RRYGGTGLGLSISKMLVSMLGGEMRLVSEQGKGSTFYVYLPFESIVVKTDTDQPEEEK
                     EIIRELERISELPANETSVVDDRESVQQGDRVLLIVEDDVQFAEILVDMAHEHQYKAV
                     VATQGDQGLQYAELLRPSAIIMDMQLPGMDGWSVLRKIRANESLAGIPVHVMSAMDRQ
                     SLGMELGANAYLRKPLDKVDLDNAFREIDDTVRHNTRRVLLIEDESIQQEIVSNLLEG
                     KDFRSEVTSVSNTQEAWTELKKAQFDCIILDLDLGNGPAEGIDFLDELKAGDEFRNVP
                     VIVFTGTEIDSAQEQSIRKHSATIVMKDGEAFNRLLSETEQFLHRVEEEQTGAATKTP
                     EKMSELLRGKRVLLVDDDMRNIYAMTSVLEDQGMVVIPATNGREAITLLTQQPDLDIV
                     LMDIMMPEMDGYEAMTEIRKVDRWKTLPIIALTAKAMVGDREKCLQHGATDYISKPVN
                     VEQLLSLLQVWLYKQ"
BASE COUNT         1367 a         1725 c         1524 g         1548 t
ORIGIN      
        1 aattagcgtt ggtacataaa ttctcgggga aacgcgacac cattaagctg ttagacatag
       61 gctgtggcac cggagagttc cttgccagtg caaaggcttc cggaatgcaa acgaagggga
      121 tagagcccag cgccgccgca agggagcatg cgatacataa ccatcaactg aatgtagaag
      181 gggaggaggg cttggagaaa ctgacagaat cctatgatgt gattaccctt tggcatgtac
      241 tggaacacgt acacgatctt cgggtccgag ttcaaaaaat cgccgaaatt ttaaacaatg
      301 ggggagtagc catcatagcg gttcccaacc ccaactccac ggatagaaat acgtataata
      361 aagaatgggc tgcctgggac gtaccgagac atttgtacca cttcacgccg accacgatca
      421 aacggctctt caatgacttt tcattgaagc acgaagcgtc aattccaatg aaatacgacg
      481 cgtattacgt cagcatgctg agtgaacgct atcgtaataa tggcaaaaac aacttgcccg
      541 ctgccttcct gaatggctgg aaatccaaca tccacgccgg atccaatgcc gagctatact
      601 ccagcgtgat ctacatcttt agaaaatcct gaagactact ttttccaggt gaagctgaag
      661 gttgttccgc taccaacttc tgaagcaatg gtcactgcgc cctcttgctg ttcaataatc
      721 ttctttacga ttgtcaaccc aatgccggta ctttcacact ggtccttggg ttgaagcgtc
      781 tggaatatgc caaagatttt ttcctgatgc tcgggacgaa ttccaacacc attatccgtt
      841 acatgaaacc gatagtgtga gccattatcc tcaaatgcaa tcttgatccg aatctcggct
      901 ttatcgttgt acttgattcc gttaatgatc agattctgaa gcacttcgtg aagtttcacg
      961 cgctcggcca aaatcaccgg caggttgtcc ggagtctcta tgaccacatt tgatgccgga
     1021 gcaataagat ctataatgct gttaatcagc tcattaagat caacatcctc ccggtcaccc
     1081 cgattattgg ccatacggga atagtggaga atgccatcga tcaattgttg catgcgtgct
     1141 gtacggctct tcatcatatt caagagctcg acaacttcca ccttcggttc ggcaccaagc
     1201 tcttcttcca tgaaactcgc aagactggcc aggccccgca acggcgcctt gagatcatgt
     1261 gaaacaatgt agacgaactc gcccaactgc ttattcacac tctcgagccg ctcagcattt
     1321 tcacgcaact caatcttctg tcgatagagc ttttcaaata cagcaacttt tgcctttacg
     1381 acattaacat ccaggggctt gtgcagatag tcgacggcgc cttcctcgta gccctgcatg
     1441 gtataacgct cctccttact cagggcagtt acgaagatga tggatatatc gcgggtcttg
     1501 ctattctctt tcaacaggcg cgcaacctcg aatccatcca tctccggcat ttgcacgtcg
     1561 agtagcataa cggcaatgtc ttccttcagc gcgattcgca gcgcctcgtt tccgctctcc
     1621 gctttcagga tctcacgacc cggaccttcc aggatgcttt ctaaggagag gatgttctcc
     1681 gggcgatcat ctacgatcag caatttgaca ggacgaacct gtgttgcggt atccgggcgc
     1741 gagggggaaa cagtggcttg cataagaatg aaaatggtct tttctgggtt tgaaaattac
     1801 gatggtgtag agatggcggc ggtaaatatc cgcgctgaac ttacagtttc tggaatcgga
     1861 cttttcggaa gattttatgg ctgccgtcga tcgttacaaa cgatgttcgc atacgattaa
     1921 aacgcaggct ctctttattg cctatggcaa agtatccgag gttcacaagc gactcgtgaa
     1981 acaaacgggt ggcgcgttcc tgcaacagcg gatcaaagta gatcatcaca tttctgcaga
     2041 ggatcagatg aaactcgttg aagctggcat cggctgcaag gttgtgctga tggaaggaaa
     2101 tgcgcctccg caattggtcc ttcatcacga ttcttccgtt aaccggggca cagtagtcgg
     2161 ttaactgacc tcgtccacca gcctgacggt agttacgggt ataatgtgag aacagggcag
     2221 gggagaagct gccttcttgc gcctgttcca atacatcggt attgatatcg gtcgcgtata
     2281 ttcttgcctt tcgcagcagg ttaacctcat ccagcatgat ggccatggag taaacttctt
     2341 ctccggtgga acatccggca tgccagatgt tgatcctggg ataggtcgcc agaaacggca
     2401 acacttgttc gcgaatggcc cggaagaatt ccggatcgcg aaacagctcg gtgaagtgaa
     2461 ccgtgagcgt attaacgagt cgggtagtca gtgcgggatc atgttccagc aacaagggca
     2521 aggccaacaa gtcgggaaga ccctcccgtt gcaaaaacac ccgcagccgc cgtacaatag
     2581 atggtcgcgc atagtgaatg aagttcattc cggtcctgct gtgcaaaacc gaaagaacct
     2641 ggtcgacgcg ctcatccgta aggacgatgg actcgcttat tgcttgtaca gccatacctg
     2701 gagcagggat aacaactgtt ctacattgac cggcttggag atgtaatcgg ttgctccgtg
     2761 ctggagacac ttttcccggt cacctaccat ggccttggcc gtgagcgcaa tgatcggcag
     2821 cgtcttccaa cgatctactt tccggatctc cgtcatggct tcatagccat ccatttccgg
     2881 catcatgata tccattagta caatatccag atcaggttgc tgcgtgagca gcgtgatcgc
     2941 ctcccgtccg tttgtggccg ggatgacgac catgccctgg tcttccagca cgctggtcat
     3001 cgcatagata ttacgcatat cgtcatctac gagcagtacg cgcttaccgc gtaacaactc
     3061 gctcatcttc tccggggtct tggttgcggc gccggtttgc tcttcttcca cccggtgcag
     3121 gaactgctcc gtctcactca gcaggcggtt aaaggcttcg ccgtctttca tgacaatggt
     3181 cgcgctgtgc ttgcggatcg attgctcttg agcagaatcg atctccgtac cggtgaagac
     3241 gatcacgggc acattgcgaa actcatcacc ggccttcagc tcatccaaaa agtcgattcc
     3301 ctctgcgggt ccattaccaa gatccaggtc gagaatgatg cagtcgaatt gcgctttctt
     3361 caattccgtc caggcttcct gtgtattcga cacgctggtc acttcggagc ggaaatcctt
     3421 tccctccagc agattcgata caatctcctg ctgtatgctt tcatcttcaa tcaacaaaac
     3481 acggcgcgtg ttgtgtcgga cggtatcgtc gatctcccgg aaggcattat ccaggtcaac
     3541 tttatccagc ggctttcgca aataggcgtt ggcgcccagt tccattccta aactctggcg
     3601 gtccatggcc gacatgacat gcaccgggat tccggcaagg ctttcattgg ctcggatctt
     3661 tcgcaaaacc gaccaaccgt ccattcccgg aagctgcata tccatgatga tcgccgacgg
     3721 ccgcagcaat tcggcatatt gcaagccctg atcaccctgc gtagccacaa cggccttgta
     3781 ctgatgctcg tgagccatgt cgaccaggat ctccgcgaac tgtacatcgt cctctacaat
     3841 gagcaacacc cggtcgcctt gctgcaccga ttcacggtcg tccacaaccg atgtttcatt
     3901 ggccggcaac tcgctgatcc gctccagttc ccggatgatc tccttctcct cttccggctg
     3961 atcggtatcc gttttcacta cgatggattc gaatggcagg taaacataga aggtgctgcc
     4021 cttgccttgc tcggaaacca aacgcatctc accgccaagc atggagacca gcatcttgct
     4081 gatcgagagg cccaatcccg taccgccata gcggcggctg gtggaaccat ccacttgctg
     4141 gaacgcttcg aagatcaatt gctgcttctc ctgcggaatg ccgatgccgg tatccgtcac
     4201 cgaaaattcg atcacctcgc gtgacttcat cagcgcggga ttggtgaacc ggacgttcct
     4261 gtcgggacga cgcatgcgga gcgacacctt gcctccctgc ggggtgaatt tgaacgcgtt
     4321 cgatagcatg ttcttgatca cttgctccag gcgcacgcga tcggtaatga tcttctcggg
     4381 cagattggtt tcctgctcca gctcgaactc gatctttttc tcgttcgcga cttcgatgaa
     4441 gagcgaacgt aaatcgtcgc gaatgatgcg cagctccgta ctttccaaca gcagctccac
     4501 cttaccggct tcgatcttgc tcaggtccag aatgtcgttg atcaggacga gcaggtcact
     4561 accggatttg tgaatgacgc gcgcgtattc gatctgctta tcggtcaggt tcgtatcctt
     4621 gttctccgat aagagtttcg ccaggatcaa tacgctgttg agcggcgtac ggagttcgtg
     4681 tgacatattg gccaggaact cggacttgta tttgctgttc tgctccagct cgcgtgcctt
     4741 tagactcagg gcttcgcgtg cctgttccag gatctcgttc tgatgctcca gcgcgtgggc
     4801 gttttccttc agctccaggt tcgactgctt cagctcttct tccgaagcct gcaacaagcg
     4861 tgactggttc gatagttctt cgttagtctg gcgcaattcc tcctcttggg cggtcaaccg
     4921 ctcggcctgc tttcgggttt cttccagcag ctttcgggtc agcatgttcg cctgtacgga
     4981 agcgatcgaa ctggcgatca gttcgccgat tccctccagg aagccgaccg tgtttcggtc
     5041 aaacgcccgg aagctcgcca cttccacagc accgaacacc tgcccgtggt aactcaacgg
     5101 cacgatgatc acatgcgagg cgtgcgcgtc gccaaggccc gattggatcc gcgcgaaatg
     5161 aaccggcgcg tccttcagcc agatactctt cttgtctttg atacactgtc ctaccaggcc
     5221 ctctcccagt tccagttgcg gcttcgcttt ggcgcgcccg tccagggcat agctgccctg
     5281 aagctggatc caactcacgt cgcggaggtc gtcgcgttcc acgaggtaca gggcaccatg
     5341 atacgcctcc aggtgtccca cgatcgaaga aacgagctca tcgctgagtc ggtggatgtc
     5401 ttcgctgtgc tcccgcaagg tgcggttgac ctgggcgata ccggcactgg tccagtggcg
     5461 ctgccggttg tcctcatcgg cagtcttcag gctatcgcgc atctgcacca gggcgttgcc
     5521 cagctcgtcc ttatcactca tcggctggaa ttgttcttcg aactttcctt ctcctatggc
     5581 atgggcaaag cccgtcgtct tccgcaggct ttccgcgagc tttatgaccg cttcactcat
     5641 ttgaccaacg gcattcttcc ttggtcgaag attcacctcc ggtatttcgc ctttacccat
     5701 ctgcaacacg tagttcctca gcttcatggt cggaacgatg atgttgttcg acatgtagaa
     5761 ggccgccatg agcaccacga tcacgatcag gatggcgatc ccgagtacgc tccacatcag
     5821 ggcccggctc gaggccatca tgccggcgtg ttctacttcc gcgagcgccc gtttctgaac
     5881 gatgacttta ttgagggaat tgatgatacc attcgttctg ggtaagatct cattatcgac
     5941 cacttcctcc gcggcgaaat gcttcatcgg atcctcgtag tcgtcaaaac tcaccagcag
     6001 gttcattacc tgtcgctggt aaacgagaac ctgttcgaaa ttcttgaaga tggcattgac
     6061 gctatcgacc attcccggct cttcccaggt agaagccagc gcaaggatat tgcccttgag
     6121 atcgggatac tcgatgtttt gcaggctctt cagtcgctcc ttgt
//
LOCUS       DAOTON010000048         6097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_11065_length_6097_cov_3.788812, whole genome shotgun sequence.
ACCESSION   DAOTON010000048 DAOTON010000000
VERSION     DAOTON010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11065_length_6097_cov_3.788812"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..944)
                     /locus_tag="P5292_01695"
     CDS             complement(18..944)
                     /locus_tag="P5292_01695"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRS37868.1"
                     /translation="MIFSSAVDPVTSLMRWGIFTFALFCTACGFNRTYLHPTTIPINA
                     KGATLSSKWDTTRVQFQGDIRQPVFYSGKGDTLKRDFTVESTWFTTSGGHKLNGWMIR
                     PTGMKPDITLLHFHGNAGCLLSQYQALVPLVEKGFQVFAFDYSGFGMSEGEATREQVL
                     LDGRAAVDYVRSRPDVNGTRLVLYGQSLGGHLAAVVGPEKSTSLDALVIEGAFSSHDD
                     IAAEKMGFIGRLLVREQYSGKAAIGQWTKQLLVIHSTEDEVIPFRLGRELFDHAHEPK
                     TFFEIRHPHICGPEFYADEIAQRIRAMVNLAN"
     gene            complement(972..1856)
                     /gene="ygiD"
                     /locus_tag="P5292_01700"
     CDS             complement(972..1856)
                     /gene="ygiD"
                     /locus_tag="P5292_01700"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HRS37869.1"
                     /translation="MDRSEFLRRLAILPITAAAMKLGELGALAETFAPTEKMPVLFLG
                     HGSPMNAIEENEFVQGFRSVAAGLPKPRAILCVSAHWETRGTFVTAMPNPPTIHDFGG
                     FPKALFDVQYPAPGSPTLARETKALVKKTEVGLDDKWGLDHGAWSVIKHLYPKADVPV
                     IQMSLDHYQNPEYHYELARELSSLRRKGILIIGSGNMVHNLGMVAWDKLNAVDYAYDW
                     AREASEKMNRFILEGDHQSLVKFRSQGKAFDLAIPTPEHFLPLLYTLALKDEKDEVSL
                     FNDKAVAGSLTMTSVRIG"
     gene            complement(1907..2359)
                     /locus_tag="P5292_01705"
     CDS             complement(1907..2359)
                     /locus_tag="P5292_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS37870.1"
                     /translation="MIRLLRTDSSNPDFQSLVQLLDAELRQRDGEEHAFFAQFNKIDQ
                     VRQVVVAYDGEIAVGCGAVKPYDAGTMEVKRMYVRPEARGKRIAVSVLRELEKWTRES
                     GYRNCILETGERQPEAIALYTRCGYERIPNYGQYAQVATSVCFHKTLE"
     gene            complement(2474..3091)
                     /locus_tag="P5292_01710"
     CDS             complement(2474..3091)
                     /locus_tag="P5292_01710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRS37871.1"
                     /translation="MNWETLLSFGDSITFGARSYLGYPEICGDHLMRHLHKSWHVVNH
                     STNGFTTMDLQRSINPLMQNFKSLHPGIITVMIGTNDIKQKVSLADFEIAYRQLIVKL
                     RLLSVNNNVLLLHIPRFTKKVVYPYNYSMNEKVEEFNAVIERLAREHDLRSLAMSFHD
                     MDFFDGVHFNQQGCENAARQLTDYLLKDKGLEELRDRSEHLTVAR"
     gene            complement(3291..3785)
                     /locus_tag="P5292_01715"
     CDS             complement(3291..3785)
                     /locus_tag="P5292_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 domain-containing protein"
                     /protein_id="HRS37872.1"
                     /translation="MTVTQQSAKHLREVFFGGNWTWSNVKDQLSDVDLDMATYKLDGF
                     NTIAVLTFHIGYYVRAVLSVLEDQPLNASDKFSFDLPPLESAAQWNALVAQTLSNAEK
                     AAGMIAELPDSRLEAIFVEVKYGTWFRNIHGLIEHTHYHLGQIAIIKKMYRTGLGSPK
                     GENQ"
     gene            complement(3799..4560)
                     /locus_tag="P5292_01720"
     CDS             complement(3799..4560)
                     /locus_tag="P5292_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRS37873.1"
                     /translation="MTNIVEYNRKAWDAQVDKKDRWTVPVGAAEIALARKGEWSVLLT
                     PTTPVPRTWFPDLRGLRLLGLASGGGQQGPLFAAAGAEVTIFDNSPKQLEQDRRVSDA
                     HDLGIRTVQGDMRNLSVFEPDSFDLIFNPCSILFVDTLQPVWEECFRVLRPGGILMTG
                     LLNPLAYQIDEEDLKLKYAEPYSDLHSLPKEKLEAFIEKQEPLLFGHTWTDQLGGQLQ
                     AGFSLTHLFEDGWGGNHVFDQYFRAFMATRAVKPN"
     gene            complement(4557..5498)
                     /locus_tag="P5292_01725"
     CDS             complement(4557..5498)
                     /locus_tag="P5292_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRS37874.1"
                     /translation="MNIHQFHYVLAVAEFRHFELAAEKCHISQSTLSTMIAKFEEEVG
                     VRIFNRKKKPVELTLEGAMIVDQLKIVSKDIDQLFEIAREIKGEVAGDINLSVIPTVA
                     PFLLPLFLQEFSLQFPKLNIVVREETTEEIRKKLKSREIDIGIVSIPLHDKELVELKL
                     YDEPFLFYDAKHVSGKKISLKELKSKNLCLMEEGHCMRTQVAELCNPGGAKQPATLNF
                     EYKAGSIDGLIRFVKSNKFSTLLPYLSTLDFSPEQRRHLSPFSASVPFRSIGLIVHQH
                     FVKKKLLNILRDRIIARTSGLLPKLDAKSQMLNPIGS"
     gene            5721..>6097
                     /locus_tag="P5292_01730"
     CDS             5721..>6097
                     /locus_tag="P5292_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015270751.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HRS37875.1"
                     /translation="MNGEAKCPFHNGTVTHSGASTNSHWWPNQLNIGILHQHAPASNP
                     MDKGFNYAKAFKQLDYPALKKDLVKLMTDSQDWWPADWGHYGGLFIRMAWHSAGTYRI
                     ADGRGGGGTGNQRFAPLNSWPDNG"
BASE COUNT         1440 a         1657 c         1523 g         1477 t
ORIGIN      
        1 gcaacatcat agcgggtcta attggccagg ttcaccatgg cgcgaatgcg ctgtgcgatc
       61 tcatcagcat aaaattcagg accgcagatg tgtggatggc ggatttcgaa gaaggtcttc
      121 ggctcatgcg catgatcgaa taattccctt ccgagtcgaa acgggatcac ttcatcttcc
      181 gtgctatgga tgaccagcaa ctgcttcgtc cattgcccga tcgccgcctt gccgctgtat
      241 tgctcgcgca caagcaggcg cccgatgaag cccattttct ccgcagcgat atcgtcgtgg
      301 gaggagaaag cgccctcaat gaccagagcg tcgagtgagg tcgatttctc gggaccgacc
      361 acggcagcca ggtgaccgcc gagcgattgt ccgtataaga cgagcctggt accgttcaca
      421 tcgggacgtg atcggacgta gtcgactgcc gcacgaccat ccagcaaaac ctgttcgcgg
      481 gtcgcctcgc cttccgacat accgaaacct gaataatcga aggcgaatac ctggaatcct
      541 ttctcgacta gtggcaccaa ggcctggtat tgtgataaca gacatcctgc gttgccatga
      601 aaatgcaaca gggtaatatc cggtttcatt ccggtcggac ggatcatcca accgttcagt
      661 ttgtggcctc cggatgtggt gaaccaggta ctctccacgg taaaatccct tttcaaggtg
      721 tcgcctttac cggaataaaa aaccggctga cgaatatccc cctggaattg aacgcgtgta
      781 gtatcccatt tggaactgag tgttgcaccc tttgcgttga tcgggatcgt tgtgggatgc
      841 agataggttc tgttgaaccc acatgcggta cagaataatg cgaacgtgaa aatgccccaa
      901 cgcatcaaac tggttacagg atcaaccgcg ctggaaaaaa tcattttgtt ctggattacg
      961 gtattggcgc atcatccgat ccttaccgat gtcatggtta acgacccggc gacagccttg
     1021 tcattgaaga gacttacttc atccttttca tccttcaacg cgagcgtata aagaagcggt
     1081 aaaaaatgtt ccggagtcgg aatggccaga tcaaacgctt ttccctggga acggaatttt
     1141 accagcgact ggtgatcgcc ttccagaatg aagcgattca ttttctcact tgcctcccgg
     1201 gcccagtcat aggcgtaatc gacggcattg agcttatccc aagccaccat gccgaggttg
     1261 tgtaccatgt ttccgctgcc gatgattaag atccccttgc ggcgcaagga cgacaactca
     1321 cgggccagtt catagtgata ctccggattc tggtaatgat cgagactcat ctgaatgacc
     1381 ggcacatccg cctttgggta taggtgcttg atgaccgacc atgctccatg gtcgaggccc
     1441 catttgtcgt cgagcccgac ttccgtcttc ttcaccaacg ctttagtctc ccgcgccagt
     1501 gtcgggcttc cgggtgcggg atactgcacg tcaaacagcg ctttgggaaa tccaccgaaa
     1561 tcgtggatgg taggaggatt gggcatggcg gttacgaatg tcccgcgagt ctcccagtgc
     1621 gcggacacac acaggattgc acggggcttg ggcaatccgg ccgctacgga ccggaaaccc
     1681 tgcacgaatt cgttctcctc gatcgcgttc atgggactgc catgtccgag aaaaagtacg
     1741 ggcattttct ctgttggcgc gaatgtctcc gccagtgcgc ccaattcacc aagtttcatt
     1801 gctgctgcag taattggcag gatcgccagt cgtctaagaa attcggatcg gtccatggaa
     1861 ctgaccttgc tgattcttta aaggtacgga atccgttttg gaagcgtcat tccagtgtct
     1921 tatgaaaaca aacgctggtg gccacctgcg cgtattgccc atagttcgga atccgctcat
     1981 acccgcatcg cgtgtaaagg gcgatcgctt cgggttgacg ctcgccggtc tccaagatgc
     2041 aatttcgata accggactcc ctggtccatt tttccaactc ccgcaggacg ctaacggcta
     2101 ttcgcttgcc acgcgcttcc ggccgtacat acatgcgctt cacttccatg gtaccggcat
     2161 cgtagggttt gaccgctccg cagcctactg ctatttcacc gtcataggct acgaccacct
     2221 gccggacctg gtcgatctta ttgaactgcg cgaagaacgc atgttcctcg ccgtcgcgct
     2281 gccgtaactc cgcatcgagg agctgaacca gtgactggaa atccggattg gaagaatcgg
     2341 ttctaagaag acgaatcata cttgacgccg gggaattagc attgaatcag ttcaacttct
     2401 gcttttcaac caatcgcttc agtaatgcga tttgtcccaa atgataatgc gtatgctcaa
     2461 tgagcccggt tcgtcatcgt gcgaccgtca aatgttcgga acgatcccga agttcttcca
     2521 gccctttgtc tttgagcaaa taatccgtca attgcctggc ggcgttctcg catccctgct
     2581 gattgaagtg aacaccgtcg aagaaatcca tgtcatggaa gctcatcgca agtgaccgga
     2641 ggtcatgctc acgagccagt cgctcgataa cagcattgaa ttcctcgacc ttctcgttca
     2701 tggaataatt gtatgggtag acgactttct tcgtgaagcg cgggatgtgc agcaacagta
     2761 cattgttatt cacagaaagc aatcggagtt tgacgatcaa ttgccgatac gcgatttcaa
     2821 aatcggccag tgataccttc tgttttatat cgttggttcc gatcatcacg gtgatgatgc
     2881 ccgggtgtaa acttttgaaa ttctgcatga gcggattgat ggatcgctga aggtccatcg
     2941 tcgtgaatcc attggttgaa tgattgacca catgccagct tttatgcaaa tgtcgcatga
     3001 gatgatctcc acaaatctcc gggtaaccca aataactgcg ggcgccgaat gtaatgctgt
     3061 caccgaatga taaaagtgtc tcccaattca tggaatatgt tttagaatct ggttagggga
     3121 atattcctgc ttctgccagg caattggaaa ctgattcaag ataggctcaa ggaatgaaag
     3181 ataggatttt aacattatcc gtactgtcac cagagcatca gtgatgaaaa tacatgacgc
     3241 aaatatgacg aaatctcagc aagggcggtg ccataagggc ttgccgcgga tcactgattt
     3301 tcgcccttag ggctcccgag gccggttctg tacatttttt taatgatcgc gatctgtcca
     3361 aggtgataat gcgtatgctc aatgagcccg tgaatattcc ggaaccaggt cccgtatttc
     3421 acctccacga agatcgcttc caaccgactg tcgggcagtt cagcgatcat accggctgct
     3481 ttttccgcat tcgatagcgt ctgggcgacc agggcattcc attgcgctgc cgattccagg
     3541 ggcgggagat cgaagctgaa tttatcactc gcatttaaag gttgatcttc caacaccgac
     3601 agcaccgccc ggacataata gccgatatgg aaagtgagca ccgcgatggt gttgaaaccg
     3661 tcgagcttgt aggtcgccat gtccagatcg acatccgaca attggtcctt tacattggac
     3721 caggtccagt tgccgccgaa gaaaacttcg cgtaagtgtt tggcggattg ttgtgttacg
     3781 gtcatctgcg gtgaagattc aattcggttt tacggcacgc gttgccatga acgccctgaa
     3841 gtactggtca aaaacatgat tcccgcccca tccatcctcg aacagatgcg tcaggctgaa
     3901 acctgcctgc aactgacctc ccaactgatc ggtccaggtg tgaccgaaca acaggggctc
     3961 ctgcttctcg atgaaagcct cgagtttctc tttaggaagg gaatgcaggt cggaataggg
     4021 ttcggcatat ttcaatttca gatcctcctc gtcgatctgg tacgcgagtg gattcagtaa
     4081 gccggtcatg aggatgccgc cgggtcgcaa cacgcggaaa cattcctccc agaccggctg
     4141 caaggtatcc acgaacagga tcgaacaggg attgaagatc aggtcgaatg aatccggttc
     4201 gaaaaccgac aagttccgca tatccccctg gacggtacgg atgcccagat cgtgtgcatc
     4261 actcactcga cgatcctgct ccaactgttt gggtgaatta tcgaagatgg taacctccgc
     4321 accggccgca gcgaagagcg gaccctgctg tcctcctccc gaagccaggc ccaacagccg
     4381 aaggccacgc aggtccggga accaggtacg cggcacggga gtagtcggcg tcagcaagac
     4441 ggaccattcc cccttacggg ccaacgcgat ctcggccgct ccaaccggga cggtccagcg
     4501 atctttctta tccacctgcg cgtcccaggc cttgcggttg tattcgacga tattggtcat
     4561 gatccgattg gattaagcat ctgcgacttg gcgtccagtt tcggcagtag gccggacgtt
     4621 ctggcgatga tccggtcacg caggatgttc agcagtttct ttttcacgaa atgttgatgc
     4681 acgatcaacc cgatcgaacg gaacggcacc gaagccgaga acggactgag gtgtcgcctt
     4741 tgctctggtg aaaaatccag ggttgacagg tacggcagca aggttgaaaa cttgttcgac
     4801 tttacaaacc ggatcagccc gtcaatggat ccggccttgt attcaaaatt cagcgtagcg
     4861 ggttgtttcg cgccacccgg attacaaagc tccgccacct gggtgcgcat acagtgcccc
     4921 tcttccatca ggcagaggtt cttcgacttc aattccttca aggaaatttt ctttccggaa
     4981 acgtgcttcg catcgtaaaa cagaaacggc tcatcgtaga gtttcagttc gaccagttcc
     5041 ttgtcatgca agggaatgga aacaatgccg atatcgatct cgcgcgattt caactttttc
     5101 ctgatctcct ccgttgtttc ttcccgcacg acgatgttca gtttcggaaa ttgcagcgaa
     5161 aactcctgaa gaaagagcgg caacaggaag ggggcaaccg tggggatcac cgacaggttg
     5221 atgtccccgg ccacttctcc ctttatctcc cgggctattt cgaacaattg atcgatatcc
     5281 tttgaaacga tcttcaactg gtcgacgatc atggcacctt ccaacgtcaa ctcgaccggc
     5341 tttttcttgc ggttgaagat cctgacgccg acctcttctt cgaatttcgc gatcatcgta
     5401 ctgagcgtcg actgactgat gtgacacttc tccgccgcca gttcgaaatg ccggaattcc
     5461 gccaccgcca gtacatagtg gaactgatga atgttcatct attttatcaa tattatgatc
     5521 taaaatatag tttttatcaa tcgaatgtac tgattacttt tacttcacca aacgagccac
     5581 atcccgataa ccgggttgat ccgaaattgc ccgcttatat ttcctaactt catagggcaa
     5641 tttcgttctg ctcatttttc acggaacaac acaaatcaac tatgtcgaac aacgcctcat
     5701 cccccgtcgc ttctccgcac atgaacggtg aagccaaatg cccgttccac aacgggaccg
     5761 ttacccattc cggcgcctct accaacagtc attggtggcc caatcagtta aacatcggga
     5821 tcctccacca gcacgccccg gcttcgaatc cgatggataa aggattcaac tacgccaaag
     5881 cgttcaaaca attggactac ccggccctga agaaagacct cgtcaagctg atgaccgatt
     5941 cgcaagactg gtggccggcc gactggggtc actacggcgg gctcttcatc cgcatggcct
     6001 ggcacagcgc cggcacgtat cggatcgccg acggtcgcgg tggtggtgga accggcaatc
     6061 aacgctttgc tccgctcaat agctggcccg acaacgg
//
LOCUS       DAOTON010000049         6071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_11136_length_6071_cov_3.543551, whole genome shotgun sequence.
ACCESSION   DAOTON010000049 DAOTON010000000
VERSION     DAOTON010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11136_length_6071_cov_3.543551"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1908)
                     /locus_tag="P5292_01735"
     CDS             complement(<1..1908)
                     /locus_tag="P5292_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRS37876.1"
                     /translation="MLQRIIRFSIENKLIIGLLVLGLVVWGAYSASRLPIDAVPDITN
                     NQVQIITVSPALGAGDIERLITFPIEQATRNIPGIIEQRSFSRFGLSLVTIVFDDATD
                     VYWARQQVNERLQTVRNEIPDGSGNPELAPVTTGLGEIFQYVVRAKPGYEGRYSELDL
                     RTIQDWIIRRQLLGTPGVADVSSFGGKVKQYEVAIDPNRLRSYGVSLETVFEALHANN
                     QNAGGSYIEKGPNALFIRTEGLVQSKTQIAGIPVKRLSDGSSLKIGDLGEVRLGHATR
                     FGALSYNDEGEVAGAVVMMLKGENSSAVIKAVKDRIREIETTLPEGVTIEPFLDRTKM
                     VNNAISTVERNLLEGALIVLFILVIFLGNLRAGLLVASVIPLSLLFAIILMNAFGVSG
                     NLMSLGALDFGLIVDGAVIIVEAVLHRLHHGKHFRGHGMVTRSEMNEEVGTAAGKLMN
                     AAVFGQIIILVVYLPILSLESIEGKMLKPMAQTVAFAILGAFLLSLTYIPMMSSLILS
                     RKIRTKPNLADRILRRLEHWHETAMNRVLKKPISVLLLAILLFGGSISVLASLGGEFI
                     PELEEGDFAVDTRVLTGSSLSTSVAAVQQAAGLLKQRYPEVEKVVTKTGSGEIPTDPM
                     PIEASDMMVILK"
     gene            complement(1997..2377)
                     /locus_tag="P5292_01740"
     CDS             complement(1997..2377)
                     /locus_tag="P5292_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37877.1"
                     /translation="MLRATSFFLSLSFLLTGADGIELFKLPGLISHYLEHRMEQPGMG
                     VDDYLALHYNTRHADQPEHDENLPFKSHEHSTACSQLVASSGEVREFSIRLFSPVINR
                     IHIPLDVKIRHADGNGVWQPPRLG"
     gene            complement(2448..4328)
                     /locus_tag="P5292_01745"
     CDS             complement(2448..4328)
                     /locus_tag="P5292_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HRS37878.1"
                     /translation="MTTSSNDSLSEVHGSVDTTVHKGRFRKLLSFLGPAYLVSVGYMD
                     PGNWATDIAGGSRFGYALIWVLLMSNLMAVLLQSLSARLGIVRGRDLAQASRETYSRP
                     VNLFLWFLAEIAIAATDLAEVLGMAIGIQLLFGLPLIYGVLLTVLDTFLLLFLLNFGI
                     RKIEAFILSLISIIGLSFLTEMILAKPDLSEVVTGFVPSLPNEAALYIAIGIIGATVM
                     PHNLYLHSSLVQTRRIDRSNSGIRRAIRFNFIDSVIALNLAFFVNAAILVLAASTFFR
                     NGMFTVSEIQDAHHFLEPLLGSKLAPVLFAVALIAAGQSSTVTGTLAGQIVMEGYLHL
                     RLSPWIRRIITRLVAIIPALLTIHYFGESATGELLVLSQVILSLQLGFAIIPLIHFTS
                     DKEKMGDFRISWWVKSLAWLSATIIVGLNVRLVIDEISAWLSASADPTLLAWTVIPLA
                     LSVGALLLYVTIKPLFPRIVRPSARAPHGGPRTLGQVETIRYSRIAITVDFSQSDEAS
                     IASALGQGGKDATYCLIHVVESAGAMVMRQDIRDMEMLSDRKNLEKYQEDLSAKGFKV
                     EYRIGFGTTRKAIPDLVKDFQPDLLVMGAHGHMGFKDLLFGTTVDKVRHRVQIPVLVV
                     RP"
     gene            complement(4338..5057)
                     /locus_tag="P5292_01750"
     CDS             complement(4338..5057)
                     /locus_tag="P5292_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRS37879.1"
                     /translation="MAHSTSEENYLKSIYHLSKSEGGSIGTNVLAADLNTTAASVTEM
                     LKRLSGKRLVDYRPYHGVSLTLAGRKAALATIRKHRLWEMFLVKSLGFGWDQVHDIAE
                     QLEHIQSDLLTDRLEAFLGHPAFDPHGDPIPDSRGRMPDRRELRLVELSPGESGILCS
                     VGTDDPEFLRQLDELGIGLGTRIQLEDRYAFDGSARIRIRPGRRQLYLAEPLMRHLFI
                     QRPAQAGKRASTKRQFTRTGK"
     gene            5210..>6071
                     /locus_tag="P5292_01755"
     CDS             5210..>6071
                     /locus_tag="P5292_01755"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRS37880.1"
                     /translation="MYRSFLKPLLFLFDPEQVHHAVFGILRNGMRIPIVRPLVRSMYR
                     VDDPGLKRTVAGLEFPNPVGLAAGFDKDARLYRELGDLGFGFVEIGTVTPRPQVGNPR
                     PRLFRLPLDEALINRMGFNNAGATAAAERLRKRRPGLIIGGNIGKNKDTPNEQAVDDY
                     LLCMEALYQVVDYFVVNVSSPNTPGLRALQEKEPLLAILRQLMKRCSDLAKGGKARPV
                     FLKIAPDLSTEQLDDIVAVVLEAGLAGVIASNTTLDRSGLKTASEQVEAIGAGGLSGR
                     PLTRKSTAVIR"
BASE COUNT         1408 a         1628 c         1740 g         1295 t
ORIGIN      
        1 tttcaggatt accatcatgt ccgatgcctc gatcggcatc gggtcggttg gaatttctcc
       61 gctaccggtt ttggtcacga ccttttccac ttcgggatat cgttgcttca agagcccggc
      121 ggcttgttga accgcggcca ccgaggtgga cagggaactt ccggtaagga cgcgcgtgtc
      181 aacggcaaaa tctccctctt ccagttccgg tatgaattca cctccaagac ttgccaacac
      241 ggaaatcgat ccgccaaaga gcagaatggc aagcaggagg acgctgatgg gttttttcag
      301 caccctgttc attgcggttt cgtgccaatg ttccaaccgg cgcaaaatcc gatccgccaa
      361 attgggtttt gtcctgatct tcctgctcag gatcagcgag ctcatcatcg ggatataggt
      421 caatgacagc aggaatgccc caagaatggc aaacgcgacc gtttgtgcca tcggcttgag
      481 catcttgcct tcgatacttt caagcgaaag gatgggtagg taaactacga ggatgatgat
      541 ctgcccgaat accgcggcgt tcatcaattt gccggcggcg gttccgactt cttcgttcat
      601 ctcagaacgg gtgaccattc catgtccccg gaaatgtttt ccatgatgca gacggtgcaa
      661 gaccgcttcg acgatgatca cggctccatc gacgatcaat ccgaagtcca acgcgccaag
      721 gctcatcaaa tttccactga caccgaaggc attcatcagg atgatggcga acaagagtga
      781 aagcggtatt accgatgcca cgagaagccc tgcccgaaga ttgcccagga agatcacgag
      841 gataaaaagg acaattagcg cgccttcgag gaggttccgt tccaccgtgg aaatggcgtt
      901 gttgaccatt ttggtccggt cgagaaatgg ctcgatggtg acgccctccg gcaaggtcgt
      961 ttcgatttcg cggatgcggt ctttcacagc cttgatgacg gctgagctgt tttctccttt
     1021 gagcatcatg acgaccgcgc cggcaacttc accttcatca ttataggaaa gcgcgccgaa
     1081 ccgggtggca tgacccagcc gaacttcccc aaggtctccg atctttagcg aactcccgtc
     1141 cgacaggcgc ttgaccggaa tccccgcgat ctgggttttc gattggacga ggccttccgt
     1201 acggatgaaa agcgcattcg gtcctttctc gatgtagctg cctcctgcgt tctggttgtt
     1261 ggcgtggagt gcttcaaata cggtttccag cgacacgcca taggagcgaa gtcggttggg
     1321 gtcaatcgcc acttcgtatt gtttcacttt tccgccaaag gaggaaacat ccgccacacc
     1381 gggcgtaccc agtagttgcc tccggatgat ccaatcctgg atggtcctca ggtccagttc
     1441 ggaataccgg ccctcatagc cgggctttgc cctgacgaca tattgaaaga tctcacccag
     1501 tcccgtggtc acgggggcca gttccggatt gccgctgcca tccggtattt cattgcgaac
     1561 ggtttgcagc cgctcattca cctgttgacg tgcccaatag acgtcggtcg cgtcatcaaa
     1621 gacgatcgta accagtgaaa gcccaaagcg ggagaaggac cgttgctcga tgatccccgg
     1681 gatgttacgg gtagcctgtt cgatcggaaa cgtgatcagt cgttcaatat caccggctcc
     1741 aagcgcgggc gataccgtga tgatctggac ctggttgttg gtgatgtcgg gaaccgcgtc
     1801 gatcggtaat cggctggcgg agtaggctcc ccatacgacg agaccgagga ccaggagtcc
     1861 aataatgagt ttgttctcga tggagaatcg aatgatgcgt tgtagcatga tgtgaaagct
     1921 tccggatcat gtgcaaaagg tccatgggcc tgatgcggat ccggtgggtt ggaaaaaaaa
     1981 tgaaaagaag aaaagatcag ccgagacgag ggggctgcca aacgccattc ccgtcggcat
     2041 gacggatctt tacatccagt gggatatgta tccggttaat gaccggagaa aaaagccgga
     2101 tgctgaactc ccgaacctcg ccggacgaag cgaccagttg cgagcaggca gtggaatgtt
     2161 cgtgggattt gaacggaaga ttctcatcat gctccggttg atcggcgtgc cgggtgttat
     2221 aatgcaatgc cagataatcg tcgacaccca tacccggttg ttccatccga tgctccagat
     2281 aatgggagat cagacccgga agtttgaaca attcaattcc gtccgccccg gtgagcagaa
     2341 aactcaatga cagaaaaaag ctggtggcac gaagcacgat gaacaaatat aaccggactt
     2401 ttcgaatgca aactgaattt ccgggttaat ttcccttaac gaaaggttca tgggcgtacg
     2461 accaaaaccg gtatttggac gcgatgccgg actttatcga cagtggtacc gaaaagcaga
     2521 tccttgaagc ccatgtggcc gtgagcgccc atgaccagca aatcaggttg aaaatccttg
     2581 accagatccg ggatagcctt acgcgtggtg ccaaaaccga tacggtactc gaccttgaac
     2641 cctttggcac tcaggtcctc ctggtacttt tccagattct tgcggtcact cagcatttcc
     2701 atatcccgga tatcctgtcg catgaccatt gcaccggcgg attctacaac atggatcaga
     2761 caatacgtcg cgtcctttcc tccttgtcct aaggcggaag caatggaagc ttcgtcggac
     2821 tgggagaagt cgacggtaat agcgattcga ctgtaccgga tggtttctac ttggccaagt
     2881 gttctgggtc caccgtgcgg cgcgcgtgca ctggggcgaa caatgcgcgg aaacagtggt
     2941 ttgatggtta cgtaaagcaa cagcgcaccc acagacaagg ccaatgggat gaccgtccag
     3001 gccagcagcg tgggatccgc actggcagac aaccaggcgc tgatctcatc gatgaccagg
     3061 cgcacattca agcctacgat gatcgtcgcg gacagccagg cgagtgactt cacccaccag
     3121 gaaatccgga agtcccccat tttttcttta tccgaagtga aatggatcag gggaatgatc
     3181 gcgaatccca attgcaggct gagaatcact tgtgacaata ccagcaactc gccggtcgcg
     3241 gactcgccga aatagtggat ggtaagcagt gcgggaatga tcgccaccag gcgcgtgatg
     3301 attctgcgga tccagggcga taatcggaga tggaggtaac cttccattac gatttgtccg
     3361 gccagggttc cggtgaccgt agaactttga ccggcggcga tcagggcaac cgcgaacagg
     3421 acaggcgcga gtttgctgcc gagcagcggc tccaggaaat ggtgcgcgtc ctggatctca
     3481 ctgaccgtaa acatgccgtt tctaaaaaag gtagaggcgg ccagtaccag gatggcggca
     3541 tttacaaaga aggcgagatt cagggcgatg acggagtcga taaaattgaa ccggatcgcg
     3601 cgtcgaattc cggagttgct gcggtcgatc cgccgggtct ggaccagtga tgagtgcagg
     3661 tagaggttgt gtggcatcac ggtcgccccg atgatgccga tcgcgatata caaggcggct
     3721 tcgttcggca gggagggaac gaagccggta acgacctcgc tcaggtccgg ttttgccagg
     3781 atcatctccg tcagaaaact gagtccgatg atggagatca gcgacagaat gaacgcctcg
     3841 atcttccgga tgccgaagtt caacaggaaa aggagcagaa aagtgtccag caccgtgagg
     3901 agaacgccat agatcagcgg tagaccgaac agtaactgaa tgccgatggc cattcccagc
     3961 acttcggcga gatcggtggc agcgatggcg atctcggcca ggaaccagag aaacaggttc
     4021 acgggtcggg aataggtttc gcgcgacgct tgagcaagat cccgtccccg gacaatccct
     4081 aatcgagccg aaagactttg gagaagtacg gccatcaggt tcgacatcag caacacccag
     4141 atcaacgcgt acccgaaacg gctgcctccg gcgatatcgg tagcccagtt gcccggatcc
     4201 atatacccca cggataccag gtaggcgggg cctagaaagg acagcagctt tcggaatctt
     4261 cctttatgta cagtggtatc gacggatccg tgaacctctg aaagggagtc gttggaagaa
     4321 gtggtcatcg tgaaaattta tttgccggtt cgcgtgaatt gccgcttcgt cgaagcgcgt
     4381 tttcctgcct gtgcgggtcg ttggatgaat agatggcgca tcagcggttc cgcaaggtag
     4441 agctggcgcc ggccgggccg gatcctgatc cgggcagagc cgtcgaaggc ataccggtcc
     4501 tccaactgga tccgggttcc cagcccgata cccagctcgt ccagttgacg caggaattcg
     4561 ggatcgtccg ttcctacgct gcacagaatg cccgattcac cgggtgaaag ttccaccaat
     4621 ctgagctcgc gacggtccgg catccgtccg cggctgtccg gtatcgggtc gccatgcgga
     4681 tcgaaagcag gatgccccag gaacgcttcc aaccggtcgg tcagcagatc cgactggata
     4741 tgttccaact gttcggcgat gtcatgcacc tgatcccaac caaagcccag ggattttacg
     4801 agaaacattt cccacagcct gtgcttgcgg atcgttgcca acgcggcctt cctgccggcc
     4861 agggtcaacg aaacgccgtg gtacggccgg tagtcgacga gccgctttcc cgaaagccgc
     4921 ttcagcatct cggtgacgga agcggcggtg gtgttcagat ccgcagccag cacattggtg
     4981 ccgatcgatc cgccttccga cttggacagg tgatagatgg atttcaggta gttctcctca
     5041 gaggtggaat gcgccatggg ataaatttag gcaaatatat aaattaaatt taggttagcc
     5101 taaaaaagtt taaccgcccc gattgctcgg attcatggcc ggtcggagac cccggatgtt
     5161 tgagctttag ccgggcaacg cattccgatt atctttgcgg ccgatgtcgc tgtaccgttc
     5221 cttcctgaaa ccactcctgt tcctgttcga cccggagcag gtgcatcatg cggtctttgg
     5281 tatcctgcga aacgggatgc gtatcccgat cgtgcggccg ctggtacggt cgatgtaccg
     5341 ggtggacgat cccggcctga aacgtaccgt agccggattg gaatttccca acccggttgg
     5401 cctggcagcc ggcttcgata aggatgcccg gctgtaccgg gaactgggtg atctcggttt
     5461 cggctttgtc gagatcggta cggtgacccc gcgaccacaa gtcggaaatc cccggcctcg
     5521 gttgtttagg ctgcctctag atgaagccct gatcaaccgc atgggtttca acaatgccgg
     5581 cgcaaccgcc gcggcggaac gattgcgaaa gcgacgcccc ggattgatca tcggcgggaa
     5641 catcggaaag aacaaggata cacccaacga acaggcggtg gatgactacc tgctgtgtat
     5701 ggaggctttg tatcaggtgg tcgattactt cgtagtgaat gtcagttccc cgaacacacc
     5761 cggcctccgc gcgctgcagg agaaagaacc cttgttggcg attctccggc aactgatgaa
     5821 acggtgcagt gatttggcta agggagggaa agccagaccg gttttcctga agatagcgcc
     5881 ggatctcagc acggaacaac tggacgatat cgtcgcggtt gtcctcgaag cgggcttggc
     5941 cggtgtgatc gcttcgaata cgacgctgga ccggagcgga ttgaagaccg cttcggaaca
     6001 ggtggaggcc atcggagccg gtggactaag cggacgcccc ctaacccgta aatcaaccgc
     6061 agttatccgc c
//
LOCUS       DAOTON010000050         6059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_11166_length_6059_cov_3.587608, whole genome shotgun sequence.
ACCESSION   DAOTON010000050 DAOTON010000000
VERSION     DAOTON010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11166_length_6059_cov_3.587608"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /gene="fbp"
                     /locus_tag="P5292_01760"
     CDS             <1..816
                     /gene="fbp"
                     /locus_tag="P5292_01760"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRS37881.1"
                     /translation="DVFANEQFISALSAGGETCAVASEENEGLINIDSEVSKNAKYVV
                     CVDPLDGSSNIDVNVSIGTIFSIYRRTSFGGIVEETDYLQRGTEQVAAGYIIYGSSTM
                     LVYTTGKGVNGFTLDPSIGEFCLSHPDMKCPKSGKIYSINEGNYAHFPDGVKKYIKYC
                     QEIDSATERPYSSRYIGSLVADFHRNLLKGGVFIYPSTHKSPKGKLRLLYECNPLAFI
                     IEQAGGKASDGFRRILDVQPTSLHQRTPLFIGSADMVTMAEEYMSRYSPVTAS"
     gene            892..4248
                     /gene="mfd"
                     /locus_tag="P5292_01765"
     CDS             892..4248
                     /gene="mfd"
                     /locus_tag="P5292_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRS37882.1"
                     /translation="MTVEELLGAYRSRPSVVSLSARLGQPERVHLHLHGLLGSADAVV
                     AAAVAGSTEVPMLFVLNDREDASYFLNDLEQLLGQEALLFFPASYKKSFQLDDPDNGN
                     ILLRAEVLSRINRGSRTLVVTYPEAIAERVVTKRNLEQNSIELRVGEKISTDFITEFL
                     LHHGFESVDFVAQAGEFSVRGGIVDIYSFANELPYRVEFFGDEVESIRTFDPESQLSV
                     QAMHHVMIMPNVQTKLLEESRSSFFEFISGNTAVWFRDAGAALSALETGLSKVKQLVA
                     AEKTYEPEQLDLVFEPAPEQEERWAKFTTVEFGKRFRFPEDHAWKFKLHPQPSFNKNF
                     NLLIANLQENKRKGLTNVLFADSARQIERLYAIFEDLEKKHGIKGHVEFTSLLLSLHE
                     GFIDEELKLACYTDHQIFDRYHRFRMRKNYSRSEAITLKELYALKPGDFVTHIDHGIG
                     RFAGLETILVNGKPQEAVRLVYKDNDILYVSIHSLHRIAKYTGKEGTTPSLHKLGSTA
                     WSTLKQKTKKKVKDIAKDLIQLYAKRRATKGFAFQPDTYLQNELEASFIYEDTPDQVK
                     STRDVKKDMERPYPMDRLICGDVGFGKTEVALRAAFKAVTDGKQVAVLVPTTILALQH
                     YNTFRERLKDFPVTVDYINRFKSAAKQKETLEHLVQGKIDIIIGTHRLLSKDVKFRDL
                     GLMVIDEEQKFGVASKEKLKAMRVNVDTLTLTATPIPRTLQFSLMGARDLSIINTPPP
                     NRYPVTTELHTFKDEIIREAINFELSRGGQVFFVHNRVSDIYDLAEMIRKLVPDVRVG
                     VGHGQLSGEQLEEVLLSFIEGELDVLVSTTIIESGLDIPNANTIIINQAHHFGLSDLH
                     QMRGRVGRSNKKAFCYLLAPPATVLTSEARQRLKAIEEFSDLGSGFNIAMRDLDIRGA
                     GNMLGGEQSGFISEIGYEMYHKILDEAIQELKETEFSDLFSEEIKEKRKFVQDCQIDT
                     DLEIRLPSDYVNSVTERLQLYKEIDSLVGEEQLVAYEAQLRDRFGPLPKPASELLNTI
                     RLRWLAEELGFEKIQLKNSRFFGNFVTNQQSPFYQSIAFMRMIRYVQKHPNRCRLKEE
                     KDKLSITIQSVPGVDDANAVLREILAYPEQ"
     gene            complement(4255..>6059)
                     /locus_tag="P5292_01770"
     CDS             complement(4255..>6059)
                     /locus_tag="P5292_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37883.1"
                     /translation="TEYPETYDVRKNEFYQIPGARGPEDKSVLAERVPTRWQKYSGGA
                     DSRLCVLLTDTASSWLGVAHGLKSFGIPFSITTDYRVALQHKVVMAYPVISGAALAPD
                     ALQALAAYPRSGGTLIGIQVLGALNEVFGFKEPVPSRQHFEVNIVNDSSAITGEFTDP
                     KELRISLGNKERFKETIGTYSYSGNQLPALAVYEDKSAAVSYKLYEGGAAYAFGFDIG
                     YFILKGHNNRHEDFHHNVVNEFEPSIDVIMRMIRNIYLSGNPDAAWIPTVPYFKNLSV
                     VITHNVNFGKALDESVEYARKEREAGMLSTYFIQTKYINDRSPFILSKDEDLTHLKQI
                     HAVGHDLQSNSVIGSPLFDQFEQGTGDETYPDYKPYVMAWDKTYHGTIFGEMRVSRYL
                     IDRIVPGNQTLAFRNSYLYTPFTYPQSLLATGYRFSSSVAANAHLTHLPFQLNYNREF
                     DSELEAFEFPVTDDDELPPYNITRAQSAIRLAKKIARYGGCFIGQVHPNALGWKVQKA
                     FYDDLKDEAWFGTLRDFGLWWTARNEVSIDITREGAQRVVTVNVPKRMEGLAIMLPIR
                     STPVAVTGGGKYSVDGKLIIFELAEGTIRITLNN"
BASE COUNT         1361 a         1580 c         1764 g         1354 t
ORIGIN      
        1 gatgtattcg ccaacgagca gttcatttcc gcgctcagcg ccggcggtga aacctgtgct
       61 gtggcatccg aagagaacga aggcttgatc aacatcgatt cggaagtctc caagaacgcg
      121 aagtatgtgg tgtgcgtcga tccgcttgat ggttcatcca acatcgacgt gaacgtttcg
      181 atcggtacga tcttctcgat ctatcgccgt acctccttcg gcgggatcgt ggaggagacc
      241 gattacctgc aacggggaac cgagcaggtc gccgccggct atatcatcta cgggtcatca
      301 acgatgttgg tctacacgac cggtaaggga gtcaacggct tcacactgga tcccagtatc
      361 ggggaatttt gcctctcgca ccctgacatg aagtgcccga aatcaggcaa gatctattcg
      421 atcaacgaag gcaactacgc gcatttcccc gacggtgtta agaagtacat caagtattgc
      481 caggagatcg attcggcgac cgagcggccg tactccagtc ggtacatcgg atcgctggtg
      541 gctgattttc accgcaacct gttgaaaggc ggcgttttca tttacccgtc cacgcacaaa
      601 tcgccgaaag gcaaactccg tctgctttac gaatgcaatc cattggcttt catcatcgag
      661 caggccggtg gaaaggcttc cgacggcttc cgccgtatcc tcgacgttca gccaacttcc
      721 ctgcaccagc gcacgccgct tttcatcggc agcgcagaca tggtgacgat ggcagaggag
      781 tacatgtcgc gctactcgcc ggttaccgcc agttaaggcg tttcctgtta gggcgattgc
      841 gttccaattc cgaccttcgc agcgccggta tttcaagtcg gcatattgcc tgtgaccgtt
      901 gaagaattgc tgggcgccta ccgctcccgg ccttccgtcg tttctctgag tgcgcgcctg
      961 ggtcagcctg agcgcgttca tcttcattta cacggcttgt tgggatcagc cgatgcagtc
     1021 gttgcagcag ccgttgccgg atcgaccgaa gtcccgatgt tgttcgtact gaatgaccgg
     1081 gaagatgctt cgtatttcct gaatgatctg gagcaattgt tgggacaaga agccttgttg
     1141 ttctttccgg catcctacaa gaaatccttc caactcgacg atccggacaa tggtaacatt
     1201 ctgttgcgtg cggaagtcct cagccgcatc aaccgcggaa gccggacctt ggtcgtgacc
     1261 tatccggaag cgattgcgga gcgggttgtc accaaacgca acctggaaca aaacagcatt
     1321 gagttacgtg tgggggagaa gatctccacc gatttcatca cggagttcct attacaccat
     1381 ggctttgaaa gtgtcgattt cgttgcgcag gcgggcgaat tttccgtacg cggcggaatc
     1441 gtggatatct actcttttgc caacgaactg ccctatcggg tagagttctt cggtgatgaa
     1501 gtggaaagta tccggacgtt cgacccggag agccagttgt cggtgcaggc catgcatcac
     1561 gtcatgatca tgccgaatgt tcagacgaag ttgctggaag agagccgatc ctcgttcttc
     1621 gagttcattt ctggcaatac cgccgtctgg ttccgcgacg cgggcgcggc cttgtctgca
     1681 ctggaaacag gcctgagcaa ggtcaaacag ttggtggcgg cagagaagac ctatgagccg
     1741 gagcaactgg atctggtctt cgagccggcc ccggagcagg aagagcgttg ggcgaagttc
     1801 acgacggtgg agttcgggaa gcgattccgt tttccggaag accatgcctg gaagttcaag
     1861 ctgcacccgc aaccttcgtt caataagaac ttcaacctgt tgatcgccaa ccttcaggag
     1921 aacaagcgga aaggactgac gaacgtgttg tttgcggatt ccgcccgtca gatcgagcgc
     1981 ctttacgcca tcttcgaaga cctcgagaag aaacacggga tcaaaggaca tgtggagttt
     2041 acctctctct tgctctccct gcacgaaggt ttcattgacg aggagctgaa gctggcctgt
     2101 tataccgatc accagatctt cgaccggtac caccgcttcc ggatgcggaa gaattacagc
     2161 cggagcgagg cgatcacgtt aaaggaactc tatgcgctga aacccggcga cttcgttacg
     2221 catatcgatc atggcatcgg ccgtttcgcc ggcctggaga ccatcctggt gaatggcaag
     2281 ccgcaggagg cggtgcggtt ggtgtacaag gacaacgaca tcctctacgt cagcattcat
     2341 tccctccacc ggatcgccaa gtataccggt aaagaaggga ctacgccctc gttacacaaa
     2401 ctcggcagca ccgcctggag cacgctgaag cagaagacga agaagaaggt caaggacatt
     2461 gccaaggacc tgatccagtt gtatgcgaag cgtcgcgcga ccaaaggatt cgcttttcaa
     2521 ccggatacgt atctacagaa cgaattggag gccagtttca tttacgagga tacgcctgat
     2581 caggtgaagt cgacgcgcga tgtaaagaaa gacatggagc gtccctatcc gatggaccgg
     2641 ctgatctgcg gagacgtcgg attcgggaag acggaagtcg cgttgcgcgc ggccttcaag
     2701 gcagtgacgg acgggaaaca agtggccgtg ctcgtgccaa cgacaatcct cgcgctgcaa
     2761 cactacaata cgttccggga acggctgaag gatttcccag tgaccgtcga ttatatcaac
     2821 cgattcaaaa gtgccgccaa acaaaaggaa actcttgagc atcttgtaca aggcaagatc
     2881 gacatcatca tcgggaccca tcgcctgttg agcaaggacg tgaagttccg cgacctcggg
     2941 ctgatggtga tcgacgagga gcaaaagttc ggtgtcgcga gcaaggagaa gctgaaggcg
     3001 atgcgggtga atgtcgatac gctgacgctg acggctacgc ccattccccg gacgctgcaa
     3061 ttctcgctca tgggtgcgcg tgacctcagc attatcaata caccgccgcc aaaccgttat
     3121 ccggtgacca cggagttgca caccttcaag gacgagatca tccgggaagc gatcaacttc
     3181 gaattatccc gcggcgggca ggtattcttt gtccacaacc gggtgagcga catctatgat
     3241 ctggctgaaa tgatccggaa actcgtcccg gatgtccggg ttggagtagg gcatggccag
     3301 ttgagtggag agcagttgga agaagtactc ctctccttca tcgaagggga attggatgta
     3361 ttggtttcta ccacgatcat cgaatcgggt ctcgatattc cgaatgcgaa cacgatcatc
     3421 atcaaccagg cgcaccattt cggtctgagc gatctgcacc agatgcgtgg acgtgtaggc
     3481 cgatcgaaca agaaggcatt ctgctacctg cttgctccgc cggctacggt cctgacttcc
     3541 gaggcgcgtc agcgactgaa agccatcgag gagttctcgg accttggttc cggattcaac
     3601 atcgcgatgc gcgacctcga tatccggggt gcgggcaata tgctgggcgg agaacaaagc
     3661 gggttcatct ccgagatcgg ttatgagatg taccacaaga tccttgacga ggcgattcag
     3721 gagttgaagg aaacggaatt ttccgacctg ttctcggagg agatcaaaga gaagcggaag
     3781 ttcgtacagg actgccagat cgatactgac ctggagatcc ggttgccgtc cgactatgtg
     3841 aacagcgtga ccgagcgttt gcagttgtat aaagagatcg acagcctggt cggtgaagag
     3901 caattggttg cctatgaggc gcaattgcgc gaccggtttg gtcccttgcc caagccggcg
     3961 agcgaattac ttaacacgat ccgtttgcgc tggctcgcgg aggagcttgg gttcgagaag
     4021 attcaattga agaacagccg cttcttcggc aacttcgtga ccaaccagca gtcacctttt
     4081 taccaatcca tcgctttcat gcgcatgatc cggtatgtgc aaaagcatcc gaaccgttgc
     4141 cgcttgaaag aagagaagga caaattgtcc atcacgattc agtcggtacc gggcgtcgac
     4201 gatgccaacg cggtgttgcg cgaaatcctc gcgtatccgg aacagtaaac cagcttagtt
     4261 attcagggtg atccgaatgg tgccttccgc caattcgaag atgatcagct tgccgtcgac
     4321 ggagtatttt ccgccgccag tgaccgctac cggtgtggag cggatcggta gcatgatcgc
     4381 gaggccttcc atccgtttgg gcacattgac tgtaacaacc cgttgagcgc cttcgcgcgt
     4441 gatgtcgatg ctgacttcat tgcgggcagt ccaccacaga ccgaaatccc gaagtgtacc
     4501 aaaccaggct tcgtctttca gatcatcgta gaacgctttc tggactttcc agccgagtgc
     4561 gttcgggtga acctgtccga taaagcaacc gccgtagcgg gcgattttct tcgccagccg
     4621 gatggcggat tgcgcccgtg tgatgttgta gggcgggagt tcatcgtcgt ccgtaaccgg
     4681 gaattcaaac gcttccagtt cggagtcgaa ctctcggttg taattcaact ggaaggggag
     4741 atgggtcagg tgcgcgttcg ccgcgaccga tgaactgaag cggtaaccag ttgcgagcag
     4801 gctttgtggg taggtgaagg gcgtgtagag gtaggaattg cggaatgcca gcgtctggtt
     4861 gcccggcacg atgcgatcga tgaggtaacg gctgacacgc atttcgccga agatcgtacc
     4921 gtggtacgtt ttgtcccagg ccatgacgta aggcttgtaa tccgggtagg tctcgtcgcc
     4981 ggttccctgt tcgaactgat cgaacaaggg cgagccgatc acggaattgc tctgcaggtc
     5041 atgaccaacg gcatggatct gtttgaggtg ggtgaggtct tcgtccttgg aaaggatgaa
     5101 cggactgcgg tcgttgatgt atttggtctg gatgaagtag gtcgagagca tgccggcttc
     5161 acgctccttg cgggcgtact cgacggattc atccagcgct tttccgaagt tgacgttgtg
     5221 cgtgatgaca acggagagat tcttgaagta cggtaccgtg ggaatccagg cggcatccgg
     5281 gttgccggag aggtagatgt tccggatcat gcgcatgatc acatcgatcg aaggttcgaa
     5341 ctcgttgacg acgttgtgat ggaaatcttc gtgccggttg ttgtgtccct ttagaatgaa
     5401 ataaccgatg tcgaatccga aggcatacgc ggccccgcct tcatacaatt tgtacgagac
     5461 ggcggcagac ttgtcttcgt acaccgcgag tgcgggcaat tggttgccgc tatagctgta
     5521 cgtaccgatg gtttccttga aacgctcttt gttgccgagg gaaatgcgca actccttcgg
     5581 gtcggtgaat tctcctgtaa tggcggaact gtcgttgacg atgttgactt cgaaatgctg
     5641 gcgggaggga accggttctt tgaatccgaa cacttcgttc aaggctccga gaacctggat
     5701 gccgatcaag gtgccgccac tgcgcggata ggcggctagt gcctgcaaag catccggtgc
     5761 cagtgccgct ccggagatca ccgggtacgc cataacgacc ttatgttgca gcgcgacgcg
     5821 gtagtcggtg gtgatgctga acgggatgcc gaacgatttc aggccatgcg cgactccgag
     5881 ccaggaggaa gccgtgtcgg tcaggaggac gcacaggcga ctatcggcac caccggagta
     5941 tttctgccag cgggtgggaa cacgttcggc caggacggat ttgtcttccg gtccgcgggc
     6001 acccgggatc tggtaaaact catttttccg gacgtcatac gtctccgggt attcggtct
//
LOCUS       DAOTON010000051         5959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_11366_length_5959_cov_2.939024, whole genome shotgun sequence.
ACCESSION   DAOTON010000051 DAOTON010000000
VERSION     DAOTON010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11366_length_5959_cov_2.939024"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="P5292_01775"
     CDS             complement(<1..173)
                     /locus_tag="P5292_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRS37884.1"
                     /translation="MDFLPVELAEYVAAHTSPEPDYLQELNRETWSKVLMPRMLSGHV
                     QGRFLSMLSCMVRP"
     gene            complement(180..1094)
                     /locus_tag="P5292_01780"
     CDS             complement(180..1094)
                     /locus_tag="P5292_01780"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HRS37885.1"
                     /translation="MRFRSFYHLLLAGLLLSSCNKEEGVLENPGNLPPISPVPASFKQ
                     NVLIEQFTQAGNGLCPASHLIVDSLVTFNPGRVWAASIHVGDPMVDSSITNLLTGANY
                     LDTLFNPSSFYPSGMLNRTVTDFSDIFPTFWPANVNAALGAIPRCGLALEASDVTGNT
                     LKLAVHVGFSDDLLGDYRLHVFLVQDAIQTSDTTYDQQNAFSQYGTSPDPASPYYLDT
                     TYLRNYRHEYVLKRIATDAGLAGDIIPQNLMSSGNEFVRTYTISLTGINRSNTRVIAF
                     VDKYGTSGTTHRIENVKACYIGEAVDWN"
     gene            1202..1804
                     /locus_tag="P5292_01785"
     CDS             1202..1804
                     /locus_tag="P5292_01785"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRS37886.1"
                     /translation="MKQFSLLLNIVLTLAVAYLYYLHYSSKSGAVTEEVKPLALPAAS
                     QQSGGILFLNSDTLLDQYDYFTEKRNQLDARQEKIRSELSTEKERLDASIQLYQQQAV
                     GMTDQERQKKEEELMMKQQSLMDRREKLLAQLDDEQAKSTEELFNRLTAFLRTYISDK
                     NVNFVLGYQKGGGILFANDSLDITQQVVDGLNKAYAAEKK"
     gene            complement(1812..2261)
                     /locus_tag="P5292_01790"
     CDS             complement(1812..2261)
                     /locus_tag="P5292_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37887.1"
                     /translation="MKRIAVVRSIGFLALLLTSRLIHAQNTGDPDRPYKNQQPHGKWT
                     IWSDSLKTKIESTGKYRNGKERGTWRYFSPTGQLLKKEHYRAKFIQTRYYHENGKRKS
                     SGKARLTMEEEYIHYFVDGKWKEFDSRGKRLATVWYNKGEEIRREDR"
     gene            complement(2258..3511)
                     /locus_tag="P5292_01795"
     CDS             complement(2258..3511)
                     /locus_tag="P5292_01795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRS37888.1"
                     /translation="MGHSSSEHFPVVILGAGPAGSGTALALARQGIRHLLVDKAVFPR
                     DKICGDALSGKVVQVLRNLDPEWINELASNQSEFVGSYGVRFVSPGGHAVDIPFSSNL
                     ASLKHPPGFLSRRLHFDAWLLKKLDATYTTFRQGWEAKSLRVTPEQAEITFHQDGQEH
                     VVTADVVVGADGDRSLVNRELNTRTIDPGHYCAGIRAYYRNVQGLHADHFIELHFLKE
                     FLPGYLWIFPMANGMANVGVGMLSSEVSRRRIDLKAMMQHAIRQHPELHARFAHAELE
                     GDVRGWGLPLGSVRRKLSGNRMMLTGDAASLIDPFTGEGIGNAMLSGEVAAGILHKAF
                     AQGEFDSTSLAAYDAEIYRRLWPEIRLSRRMQQLSRQAWLFDLVVRKAKKNPTLRETI
                     TCMFEDIDLRDRFRSPSFYFKLLFQ"
     gene            3571..4017
                     /locus_tag="P5292_01800"
     CDS             3571..4017
                     /locus_tag="P5292_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRS37889.1"
                     /translation="MVSKRFVIRVYGIYINEREEVLLSDEFIYGREVTKFPGGGLEFG
                     EGTRECLEREMMEETGHRFEVLDHLYTTDFFVPSAFDPQIQVMSIYYRMRPLEEFRME
                     TRDRPFDFVTYTENAQVFRWKSIHGLVPSDMTLVIDQKVAGLIKAS"
     gene            complement(4004..4618)
                     /locus_tag="P5292_01805"
                     /pseudo
     CDS             complement(4004..4618)
                     /locus_tag="P5292_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
     gene            complement(4746..5315)
                     /locus_tag="P5292_01810"
     CDS             complement(4746..5315)
                     /locus_tag="P5292_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37890.1"
                     /translation="MQDIEPFYNWSGYYSAAQDERSPFYGTEYSEFEYTNTVYNYYIH
                     PQWDQFGSNTLYLKILFAEYERGFAIIELIGEWNDALYNDIMFLKRDVIESMLGEGIN
                     KFILIGENVLNFHGSDDCYYEEWFQEVEDGWIAFVNFREHVAKEMNRFNLDYYTISGG
                     ELSELNWRNFLPQELFKKIDGIIRHRLVS"
     gene            complement(5361..>5959)
                     /locus_tag="P5292_01815"
     CDS             complement(5361..>5959)
                     /locus_tag="P5292_01815"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRS37891.1"
                     /translation="LGIVDASELPPTYGLEHGRVIGLIAGGDAAIRKAVEFAEDDPEK
                     GWNDLQQHDICPDDVVIGIAASGTTPYVVGALERCKQNQILTIAIACNPGTPVAKAAH
                     YPIEIIVGPEVVTGSTRMKAGTAQKLVLNMISTSVMVQLGRVQGNQMVDMQLTNNKLI
                     TRGTKMLMNALNINAIEAGDLLNKFGSVRKALAHYRRD"
BASE COUNT         1382 a         1498 c         1635 g         1444 t
ORIGIN      
        1 gggcgcacca tgcaactgag catggacagg aagcgacctt gtacatgtcc cgacagcatg
       61 cggggcatca gcaccttgct ccaggtctcc cggttgagtt cctgcaggta gtcaggttca
      121 ggagaagtat gagctgccac gtattcggca agctctactg ggaggaaatc catgccggtt
      181 tagttccagt cgactgcttc gccgatgtag caagccttca cattttcgat ccggtgcgtg
      241 gtgccgctgg tgccgtattt gtcgacgaaa gcaatgacac gcgtgttgga ccggttaatg
      301 cccgtcagac tgatcgtgta cgtacgaacg aattcgttcc cgctgctcat gagattctgc
      361 ggaatgatat cgccggccag tcctgcatcg gtggcgatgc gcttgagcac gtattcatga
      421 cgatagttgc gcagataggt ggtatcaaga tagtaaggtg aggcagggtc cggggaagta
      481 ccgtactggc tgaaagcgtt ttgttgatcg taggtcgtat ccgaggtctg aatggcatcc
      541 tggacaagga aaacatgcag gcggtagtcg ccaagcaggt cgtcggaaaa accaacgtgt
      601 accgcgagct tcaacgtatt gccggtgaca tcactggcct cgagcgcaag accgcagcgt
      661 gggatagcgc ctaaagccgc atttacattg gctggccaga atgttggaaa gatgtcgctg
      721 aagtcggtga ctgtccggtt caacatgccg gaaggataaa aggacgacgg attgaacagc
      781 gtatcgaggt aattagcccc ggtcaacaga ttggtaatcg aggaatccac cataggatcc
      841 ccaacgtgaa tactcgccgc ccatacgcgt cctggattga aggtcaccaa actgtcgacg
      901 atcaggtgcg aagcaggaca aagaccgttt ccggcctggg tgaattgctc gatcaggacg
      961 ttctgtttga acgaagcggg taccggggag atcggcggca ggttgccggg attttcaaga
     1021 acaccttcct ctttattgca gctactcaac aacaggccgg ccagcagaag gtgataaaag
     1081 gagcggaaac gcatagtgcg gatttagact cgtaaaataa gcggttctaa ccttgttttt
     1141 caaatgaaat catcggttaa tggcattgat ttcggctatc tttgcccaaa atccttcgga
     1201 aatgaagcaa ttttcactgc tcctgaatat tgtcctgaca ttagcggtag cgtacctcta
     1261 ttacctccat tattcatcca agagcggagc ggtaacggag gaggttaagc ccctcgcgct
     1321 tcctgcggct tcccagcaat caggcggtat tctattcctg aattcggata cattgcttga
     1381 ccagtacgat tacttcacgg aaaaacggaa tcaactggat gcccgacagg agaagatcag
     1441 aagtgaactt tccacggaga aggagcgact ggatgcctcg atccagttat accaacagca
     1501 ggcggtcggc atgaccgatc aggaaaggca gaaaaaagaa gaagagttga tgatgaagca
     1561 gcaatcgctg atggatcgcc gggaaaaatt gctggcgcaa ttggatgatg agcaggctaa
     1621 atcgacggaa gaactcttca accggctaac ggcatttctt cggacttaca ttagcgataa
     1681 gaatgtcaat ttcgtcttgg gctatcagaa aggcggcggc atcctctttg ccaacgatag
     1741 tcttgacatc acacaacaag tggtcgatgg gctgaataag gcatatgcgg cggagaaaaa
     1801 gtaagcccgg ttcagcggtc ttcgcgtcgg atctcttcgc ctttgttgta ccagacggta
     1861 gctaatcgct ttccacggct gtcgaattct ttccacttgc catcgacgaa atagtggatg
     1921 tactcttcct ccatggtcag cctcgctttt ccactgctct ttcgcttccc attttcgtgg
     1981 tagtaccggg tctggataaa cttggcacgg tagtgttctt tctttaaaag ttgccctgtc
     2041 ggactgaagt atcgccaagt accccgctcc tttccgttcc gatacttacc agtgctttcg
     2101 atcttcgttt tcaggctatc cgaccagatc gtccactttc cgtgtggttg ctggttcttg
     2161 taaggacgat cgggatcccc ggtgttttgt gcatgaatca atctggacgt tagcaacaac
     2221 gccaggaagc caatcgagcg tacaaccgca attcgcttca ttggaacagc aatttaaagt
     2281 agaatgacgg agaacggaat cggtcgcgaa gatcgatgtc ctcgaacatg cacgtgatgg
     2341 tttcccgcag ggttgggttc ttcttcgctt tgcgtacgac cagatcaaag agccaggctt
     2401 gcctgctgag ttgttgcatc cggcgactta gccggatctc cggccacagg cgccgataaa
     2461 tttccgcatc ataagcagcc agggaggttg agtcgaattc gccttgcgca aaggctttat
     2521 gcagtatacc agcagcgact tccccgctca gcatggcgtt gccgatccct tctccggtaa
     2581 atggatcgat gagtgaagca gcatcaccgg tgagcatcat gcggttgccc gacagtttcc
     2641 tgcgaacgga gcccaggggc agcccccagc cacgtacatc gccttccaat tcggcgtgag
     2701 cgaatcgcgc atgcagttcc ggatgctgtc ggatcgcatg ctgcatcatg gctttcaggt
     2761 caattctcct gcgggagacc tcgctactta acatgccgac acccacattc gccatgccat
     2821 tggccattgg aaagatccag agataaccag ggagaaactc ctttaaaaaa tgcaactcga
     2881 tgaaatggtc ggcgtgtaag ccctgcacat tccggtaata agcacggatg ccggcacaat
     2941 aatgacccgg gtctatggtg cgggtattca attcgcggtt gaccagcgaa cggtctccgt
     3001 cagcgccgac caccacatcg gccgttacga catgttcctg gccatcttga tggaaggtga
     3061 tttcagcctg ttccggggtt acgcggaggg atttcgcttc ccatccttgt cgaaaggtcg
     3121 tgtaggtggc atcgagtttt ttcaacagcc aggcatcaaa gtgcagacgg cgtgaaagga
     3181 aaccaggtgg atgtttcagg gaagccaggt tggaactgaa cgggatatcc acggcatgtc
     3241 cgcccggcga aacgaatcgg acaccataac tgccaacgaa ttcggactga ttcgaagcaa
     3301 gttcgttgat ccactccgga tccagattcc gcagtacctg cacgacttta ccgctcaggg
     3361 cgtcgccaca gatcttatcg cgggggaaga ccgccttgtc aaccagcaga tgccggatgc
     3421 cctgccgcgc cagtgccaga gctgtaccgg aaccggctgg accggctccg agtatcacaa
     3481 ccggaaagtg ttcagatgac gaatgtccca ctggtcaaag ttaagggaat gtctatgtaa
     3541 aacttgatcg ggtattttcg gttactttgt atggtgagta aacgattcgt gatccgtgtt
     3601 tacggcatat acatcaacga acgggaagaa gtcctgttga gcgacgagtt catttacgga
     3661 cgggaagtga ccaagttccc cggcgggggc ctcgaattcg gtgaaggtac ccgggagtgc
     3721 ctcgagcgtg aaatgatgga ggagaccggt catcggttcg aggttttgga tcatttgtac
     3781 acaaccgatt tttttgtgcc gtccgccttt gatcctcaga tccaggtgat gagcatctat
     3841 taccgtatgc gaccgctgga agaattcagg atggaaacgc gtgaccggcc gttcgatttc
     3901 gtgacgtaca cggagaacgc gcaggtcttt cgatggaaat ccattcacgg tcttgttccg
     3961 tcggatatga cgctggtgat cgaccagaaa gttgcgggac tgatcaaggc ttcctgattc
     4021 cgggccactg ttttagctgc acgaatccgt cattcctcaa tttagattcc agggcttttc
     4081 gctctttcgg atccggcggt tctgaagcga ggacacgata gtttcgatgt tcctgcgggc
     4141 cgtcaacgag gatataaact tcggacaggt ggtactccag gctgatctgg gtggccatgt
     4201 agaaggcgtg cttccagtgt tctgttgacc aaacctgcag atagtcgctt cccagttcga
     4261 ctttctgccc gaatacgtcc cagacctctc cgtcctgcat gagagaatcg gacaagggga
     4321 tccggtcgaa caggctcagt tgccggatcg tgaccggtac gaccccaaag ggaaccatgc
     4381 ccagcttgtt ggcggcagcg cgcgacaagt cgatgatgcg actcttcacg aacgggccac
     4441 ggtcgttgat gcggacgatc gccgatcggc cggtcttctt gtttgttaca agtacgatgc
     4501 tgttgaacgg tagattgcgg tgagcggctg tgaagtcgcc tttgtcatag tattctccgt
     4561 tgctggtttc ctgtccgtga aaccggtcgt gatagaagga agctttcccc gcctgcgaag
     4621 cggaatctcc tgcagcgatg acggatgata agcccggttg ctgaaggagg atgaatagat
     4681 agtgtaaaag gaagagggga tgcacggcga ggctcaagtt actcaatcct gcagcccttg
     4741 acaaatcaac tgaccaagcg atggcgaatg atgccgtcga tcttcttgaa tagttcctgc
     4801 ggaaggaagt tccgccagtt cagttcactc aactcgcctc cgctgatcgt gtaataatcg
     4861 aggttgaagc ggttcatttc tttcgcgacg tgctcgcgaa agttgacgaa cgcgatccat
     4921 ccgtcctcca cttcctgaaa ccattcttca tagtagcaat cgtcggagcc atgaaaattg
     4981 agcacgtttt caccgatgag gatgaacttg ttgatgcctt ctcccagcat ggattcaatg
     5041 acatcgcgtt tgaggaacat gatgtcgttg tacaaggcgt cgttccattc accgatcagt
     5101 tcaatgatcg cgaagccgcg ttcatattct gcgaaaagga tcttgaggta tagcgtattg
     5161 gatccgaatt ggtcccattg cggatgaatg taatagttgt aaaccgtgtt cgtgtattcg
     5221 aactcgctat actcggtgcc ataaaaaggg gatcgttcgt cttgggccgc ggagtaatac
     5281 cccgaccaat tgtagaacgg ttcaatatcc tgcatgacta ccgctaattt acaaaaaatc
     5341 agcgggagca gaacgtccga tcaatcacgc ctgtaatgtg ccagtgcttt ccgaactgag
     5401 ccaaatttgt tcaacaagtc gccggcctcg atcgcgttga tgttgagggc gttcatcagc
     5461 attttcgttc cgcgggtgat cagcttgtta ttggtgagtt gcatgtcgac catctggttt
     5521 ccctgtacgc ggccgagttg gaccatgacc gaggtggaga tcatattcag cacgagtttc
     5581 tgtgctgttc cggctttcat ccgggtgcta cccgtaacga cttccggccc gacgatgatt
     5641 tcgatcgggt agtgcgccgc cttggctacg ggtgttccgg ggttgcaggc gatcgcgatg
     5701 gttaggatct ggttttgttt gcagcgttcc aaagcgccta ccacgtaagg tgtagttcct
     5761 gatgcggcaa tgccgatcac cacatcgtcc ggacagatat catgttgttg cagatcgttc
     5821 cagccttttt caggatcatc ttccgcgaat tcgacggcct ttcgaatggc ggcatctccg
     5881 ccggctataa gtccgatcac ccgcccgtgt tccaggccat aggttggagg gagttccgat
     5941 gcatcgacta tcccgaggc
//
LOCUS       DAOTON010000052         5930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_11431_length_5930_cov_3.034213, whole genome shotgun sequence.
ACCESSION   DAOTON010000052 DAOTON010000000
VERSION     DAOTON010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11431_length_5930_cov_3.034213"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="P5292_01820"
     CDS             <1..522
                     /locus_tag="P5292_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HRS37892.1"
                     /translation="MFDGDPADPKMNSKLNYESTFAFRNYKLSTNPSEYEFSDIDNQP
                     PQRRIGRTEDMFTLFDFSAKWDPVPTMLCQNHEQVIKGFMGQTTAFKEKFIKPGVLIM
                     GETKSENEARYIHGEYGKGTWTFYSGHDPEDYQHQVGDPVTDLALHPNSPGYRLILNN
                     VLFPAAKKKKQKT"
     gene            complement(669..2531)
                     /locus_tag="P5292_01825"
     CDS             complement(669..2531)
                     /locus_tag="P5292_01825"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37893.1"
                     /translation="MIRQLFLLFFCLIAGLARSQEAKDSIAATPRVPTLIRYQTEADY
                     RDRQISERAPDTSVSRLHEVFITDNGETVHLGNTGSALNSIWFRPLTGLTTSLGINAY
                     SAYGSGPEEQSYYRTNKRYSRIDFWQGAFRESGIRFLHTQNVNRQLNLGLTIDRFTVK
                     EFLPEGDTYFNRISLFGSYESPNRRYRLFAHTSWMRIKNQVNGGLSNDSAFLAGSVDN
                     LGLKGSAIRLSDADQRIRRRELYLSQYYNFGHGDATDSLFVPWRLGWSLRYRRDSFTY
                     LDNASDSSYYADFFLKTSESYDSSLTDQLSNRIALERVSARNAAVPLSFQVYVEDQRI
                     NAYQSAATDWYNQVAGLTIRLGGDSTKIRVRLHAAQVISGRDQENREWQAQVAGKLLH
                     DLDWYAAVAQEQTAVPFIFQRYSGNHLQWDNDFTSQQVGLICAGIKIPKWRLGLTARA
                     LQLEDYLYVGYDQKPAQLSGSVNGVQAELSKEFRFRKWGFGNIVRFQSFDATDQLRVP
                     ELLLQHALFFEDALFNKALVLRTGINAWYFSEWNGAAYSPSTSLYYLQKTSTTGGYLR
                     TDLFLNLKIKTAGIFLRFTNLTDGLGAKAYELVPGYPQPGRVFHFGVRWMFFDQ"
     gene            2691..3881
                     /locus_tag="P5292_01830"
     CDS             2691..3881
                     /locus_tag="P5292_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37894.1"
                     /translation="MKRPLLIGVMLAVLQTASLTAATVYTRASGNWTSTSVWSYTDGG
                     ASCGCTPTGADVVYIRHVVNLNKHLTNGSGTLGAVTGSLNILPGGILSGSFSYDMDVR
                     SGGSLYVCGWLLIRNLSFYNGSSVNVCASGNLLVNGSFNNRNNSDNVRIDGSMTILGS
                     YTNGNGGIISGSGTIVVFFGPVSNPGDTYGCVNTNPCSSFPCSIQSPCGGTLAVSWVG
                     FTAVPDGDSVQLAWTTASEEGSVRFVVQRSLDGVHFEDRASMAASGYSSAQQHYSTRD
                     GELPEGWVYYRIREDAVVGEPSYSEVVAVRLAGSSEFTLLTDPVSGRDPQLLTASESA
                     LPMQFRLTDLFGNLVGYWEVPTEKDKTRYPVSTDLLNQTGIYVLTVTQGPRTFYGRFF
                     YAIE"
     gene            complement(3885..4073)
                     /locus_tag="P5292_01835"
     CDS             complement(3885..4073)
                     /locus_tag="P5292_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRS37895.1"
                     /translation="MQKRLVRKDKKIAGVCGGLGEYFNQDPVIFRILFVAAVLLFGTG
                     VLLYLVMAVIMPDGEKQG"
     gene            4121..>5930
                     /locus_tag="P5292_01840"
     CDS             4121..>5930
                     /locus_tag="P5292_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37896.1"
                     /translation="MPGFLSKLAETVVQQHGTDLSRIALVFPTRRAGLFFKKELAARL
                     QGPAWSPRIFAIQDFMVSLSESSIPDSVTLLFELFSVYRNYFPDEPFERFYPWGEVML
                     RDFEEIDRNLVDAEKIFTLVSDLRSIDAAFALEEDDLERFRGFWKNFFGRELSGIKSE
                     FLHTWKNLLPIYRDFRKKLQDKGQAYEGLAYRLAAEQLRGDGKPAVEFDSVIFAGLYA
                     LSKSEEAIIEHFVDQGIGATHWDTDRYYLDDPAQEAGYFLRSSPLFREPRNWVGDDLL
                     RQPKSFEVASIPLEVGQAKYAGDLLAGWVKEMGFLPERTAVVLPDEHLLFPVLYSIPE
                     DFPSINVTMGYPLRLTPLYRLLESLIALVRNRRSGKEDAGSYYFRDVEQILGHPYVRL
                     LDPEGLRDWQQRHRDKRWIRISESVLRENVPPFFSRFFKKVEGMPALFAWFREILRAV
                     LEAMQEQEFRFHRLESEFVFHFHKQLSRLEELVRDLPVEQSLETFWTLFREVVHSARI
                     PFTGEPLSGLQVMGFLEAYMKRSAGIDVHEQVAYKGTIVLGTVHQDVHSIGKDLAKTL
                     FENYGYRVVDLGVMTPCAKVLDTARRGPVEHFATKIA"
BASE COUNT         1364 a         1504 c         1634 g         1428 t
ORIGIN      
        1 atgttcgacg gtgatccggc cgatcccaag atgaacagca agctgaatta cgagagtacc
       61 ttcgcttttc gcaactataa actcagtacg aatccgtcgg agtacgaatt cagtgatatc
      121 gataatcaac ccccacagcg acggatcggt cgcacggagg atatgttcac gctcttcgac
      181 ttttcggcaa aatgggatcc tgttccaacc atgttgtgcc agaaccatga gcaggtgatc
      241 aaagggttca tgggacagac caccgcgttc aaagagaaat tcatcaaacc cggcgtgctc
      301 atcatggggg agacaaaatc ggagaacgaa gcccggtaca tccatggtga gtacggaaaa
      361 ggtacctgga ccttttacag cggtcacgat ccggaggatt atcagcacca ggtaggtgat
      421 cctgtcaccg atcttgccct gcatcccaat tccccaggtt atcgactcat cctgaacaac
      481 gtactttttc ctgctgccaa gaagaagaag cagaagacgt gaagggaact ggagctagta
      541 attagtaact agtaattggt aactagtaat tggtaactag taactagtaa ctggtaatta
      601 gtaattggta acttgtaatt agcctcaaat ttcttagtta ctaattacca gttactaatt
      661 accaattttc actgatcaaa aaacatccac cttacaccaa aatgaaacac ccgtcctggt
      721 tggggatagc cgggaaccag ttcgtaggct tttgcaccta aaccgtcggt caggttggtg
      781 aagcgcagga aaatgcctgc tgttttgatt ttcagattga ggaatagatc ggtacgcagg
      841 taacctccgg tggtacttgt tttttgaaga tagtagaggg aggtggaagg agagtaagcg
      901 gccccgttcc attcgctgaa ataccaggca ttgattccgg tacgcaacac cagcgctttg
      961 ttgaagagtg catcttcgaa aaaaagcgca tgctgcagca gcagctccgg aacgcgaagc
     1021 tgatcggttg cgtcgaatga ttggaatcgt acgatattgc cgaagcccca tttccggaac
     1081 cggaattcct tcgaaagttc cgcctggact ccgttgacgc ttccgctcaa ttgtgccggc
     1141 ttttgatcat acccgacata caggtagtct tccagttgta gcgcacgggc ggtaaggcct
     1201 agtcgccatt ttggaatctt gattccagcg caaataagtc caacttgctg ggaagtgaaa
     1261 tcattgtccc attgcaagtg gttgccggag tagcgctgaa agatgaacgg aaccgctgtc
     1321 tgttcctggg caacggcggc ataccagtcg agatcatgca agagctttcc tgctacttgc
     1381 gcttgccatt cccggttttc ctggtcgcgc ccgctgatga cctgtgcggc atgtaacctt
     1441 acccggatct tcgttgaatc cccgcccaat ctgatcgtta gccctgctac ctggttgtac
     1501 cagtccgtgg cagcggactg ataggcgttg atccgttgat cttccacgta cacttgaaaa
     1561 gacagtggca ctgcagcatt ccgtgccgag acccgttcca gtgcaatgcg attcgacaat
     1621 tgatcggtca ggcttgagtc gtaggattcc gaagttttca ggaagaaatc agcgtagtag
     1681 ctgctatcgg atgcgttgtc gaggtaggta aatgaatcac ggcgataacg taggctccag
     1741 ccaaggcgcc agggaacgaa cagcgagtcc gttgcatcgc catgaccgaa attgtaatat
     1801 tgactgaggt aaagttctct tcttcggatc cgttgatcgg catccgagag ccggatagcg
     1861 gaacctttca atccaaggtt gtcgacgctt cctgcaagga atgcggaatc attactcagc
     1921 cctccgttca cctggttttt aatgcgcatc caggaagtgt gcgcgaacaa ccgatagcgc
     1981 cggttgggac tttcgtagga accgaataag gagatccggt taaagtaagt gtcaccttcc
     2041 ggcaggaatt ccttaaccgt aaagcgatcg atcgtcagtc ccaggttaag ttgccggttg
     2101 acattctgcg tatgcagaaa ccggatgcca ctttcacgaa aagcaccttg ccagaaatcg
     2161 atgcggctat accgtttgtt ggtgcggtag taggattgtt cctccggtcc ggaaccgtag
     2221 gcggagtatg cattgatgcc gagggaggtg gtgagtccgg tcagcggtcg gaaccagatg
     2281 gaattcaagg ctgatccggt gtttccaagg tgcaccgttt caccattatc cgtaataaac
     2341 acctcgtgca atcggctaac ggaggtatcg ggtgcccgtt cactgatctg tcgatcacga
     2401 tagtcggcct ccgtctgata acggatcagg gtgggtaccc ggggtgtggc ggctatcgaa
     2461 tctttagctt cctgcgacct ggccagtccg gcaatcaggc aaaagaaaag gaggaatagc
     2521 tggcggatca tttgaaaggt gagcccgaaa tttcggtaaa tgtaagctat tttggcgctt
     2581 ttcggcctaa actaacaggt ttcgggtaaa ttctggatgc aagatgaata ggtgaatgct
     2641 tcaccggagc ctctggtatc ggttaccttg atgggtattc agcttcatcg atgaaacgcc
     2701 cattactcat cggtgttatg ctggcagtgc tccagactgc cagccttacc gccgcaaccg
     2761 tgtacacacg cgccagtgga aactggacga gtacatccgt ctggtcatat accgacggtg
     2821 gggcttcctg cggttgcacg cctacgggcg ccgacgtggt ctacatcagg cacgtcgtca
     2881 atctgaacaa acacctgacg aacggatccg gtacgctcgg ggcagtcact ggtagcctga
     2941 acatcctgcc gggaggtatc ctcagtggca gcttctctta tgatatggat gtacgctccg
     3001 gtggatcgct gtatgtctgt ggatggttgt tgatccgaaa cctgtccttt tacaatggct
     3061 catcggttaa tgtgtgcgcc tccggaaacc tgctcgtgaa tggcagtttc aataacagga
     3121 acaacagtga taatgtcagg atcgatggca gcatgaccat cctgggtagc tatacaaacg
     3181 gcaatggagg catcatctcc ggatccggaa caatcgtggt ctttttcgga ccggtcagca
     3241 atccgggtga tacgtacggt tgtgtgaaca ccaatccatg cagctcattt ccgtgcagta
     3301 tccaatcgcc ctgtggcggt accctggcag tatcctgggt cgggttcacg gccgtcccgg
     3361 acggcgacag cgtccaactg gcctggacaa cagcttcgga ggagggtagt gttcggttcg
     3421 tggtacaacg atcgttggat ggtgtacatt tcgaagaccg tgcttccatg gctgccagtg
     3481 gatatagcag cgcacagcaa cactattcaa cgcgggatgg tgaactgccg gaaggctggg
     3541 tctattatcg gatcagggag gacgcggtgg tgggagaacc ttcctattca gaagtcgtgg
     3601 ctgtccgtct agccggttct tcggaattca ccttgctcac cgacccggtg tcagggcgtg
     3661 atccgcaact tcttaccgca tctgaatcag cattacccat gcagtttcgc ttaaccgatc
     3721 tgtttggtaa cctggtaggc tattgggaag tgccgacgga aaaggataaa acccggtatc
     3781 cggtatccac cgacttgttg aaccagacgg gaatctacgt cctgactgtt acgcaagggc
     3841 cgaggacctt ttacggaagg ttcttctatg cgatcgaata aggttcagcc ttgcttttca
     3901 ccgtcaggca tgattacggc catgacaagg tagagcagta cccccgttcc aaatagcagc
     3961 acagcggcaa cgaacaggat gcggaagatc acaggatcct ggttaaaata ttcacccagc
     4021 cctccgcaga ctccggcgat ctttttgtct tttctgacga gacgtttttg catgggaacg
     4081 aaatttattc ccgaatttac gattcgattt tcattccgct atgccgggct ttctttccaa
     4141 acttgccgaa acagtcgtgc agcaacacgg gaccgatctg tcccgtatcg ccctggtttt
     4201 tccgacccgg cgggcaggcc tgtttttcaa aaaggaactc gctgccaggc tgcaggggcc
     4261 tgcctggtcg ccccgcattt ttgccattca ggatttcatg gtgtcgcttt ccgaatcaag
     4321 cattccggat tcggtgacac tcttgttcga gttgttctcg gtataccgga attattttcc
     4381 cgacgagccg tttgaacggt tttatccctg gggagaagtc atgttacgtg acttcgaaga
     4441 gatcgaccga aacctcgtgg atgcggagaa gatcttcacc ctggtatccg atctgcgaag
     4501 catagatgcc gcgttcgctc tggaggaaga tgacctcgag cgtttccgag gattctggaa
     4561 aaacttcttc ggtcgggagt tgtcagggat caagtcggag tttctccata cctggaaaaa
     4621 cctcttgccc atttatcggg acttccggaa aaagctacaa gataagggac aggcatacga
     4681 aggattggcc taccggctcg cggcggagca actccgggga gatgggaaac cggctgtgga
     4741 gttcgattcc gtgatcttcg cggggctgta tgccctaagc aagtccgaag aggctatcat
     4801 cgaacatttt gttgatcagg gaatcggcgc aacccactgg gatactgatc gctactacct
     4861 cgatgatccc gctcaggaag ccgggtactt cctgcgcagc agtccgctat tccgggagcc
     4921 gcgtaactgg gtgggcgacg atctgctccg tcaacccaaa tcattcgaag tagccagcat
     4981 tccactggag gtgggacaag cgaagtatgc cggagacctc ctggccgggt gggtgaagga
     5041 aatgggtttt cttccggaac gcaccgcggt cgttttaccc gatgagcact tgctgtttcc
     5101 ggtgctgtat tcgatacccg aagatttccc ctcgatcaat gtcacgatgg gctatccctt
     5161 gcggctgacg cctttgtatc gcctcctgga atcgctcatc gcgctcgtgc gcaaccgcag
     5221 gtccggtaag gaggatgccg gaagttatta cttccgggat gtcgaacaga tcctcggcca
     5281 tccgtatgtc cgattgcttg acccggaagg attgcgtgac tggcagcaac gtcatcgcga
     5341 caaacggtgg atacgcatca gcgagtccgt cttgcgtgag aacgtgcctc cgttcttctc
     5401 ccggttcttc aagaaggtcg aaggaatgcc cgcgctgttc gcctggttcc gtgagattct
     5461 ccgtgccgtg ctggaagcca tgcaggaaca ggagttccgc tttcaccggc tggagtccga
     5521 atttgtcttt catttccaca agcaactcag caggctggag gagttggtac gtgacttgcc
     5581 cgtcgaacag tcgctcgaaa cgttctggac gctcttccgc gaagtcgtgc actcggcaag
     5641 gattcctttt accggtgaac cgttgagcgg tctgcaggtg atggggtttc tcgaagccta
     5701 catgaagcgg tcggcgggca tcgacgtgca tgaacaggtc gcgtacaaag gcacgatcgt
     5761 gctggggacc gttcaccagg acgtgcattc gatcggaaaa gacctcgcga agacgctgtt
     5821 cgagaattac gggtaccggg tggttgatct gggcgtgatg acgccgtgcg cgaaggtgct
     5881 cgacacggcg cgccgcggtc ccgtcgagca cttcgcgacg aagatcgcga
//
LOCUS       DAOTON010000053         5904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_11492_length_5904_cov_2.953325, whole genome shotgun sequence.
ACCESSION   DAOTON010000053 DAOTON010000000
VERSION     DAOTON010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11492_length_5904_cov_2.953325"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="P5292_01845"
     CDS             <1..863
                     /locus_tag="P5292_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HRS37897.1"
                     /translation="QAEMDSLGQTGTTVAIPYELNLDDQLFRYGARVNTDLVMDLQAA
                     PIPVVTGYVGNQPKQELLPWYYFPLLESTDNHPIVRNLNAVKTEFPSSVDTIDVTGIK
                     KTVLLAGSQYCRLQLPPARISMNILQQEPDPATFPRRHVPIVLLLEGNFRSLYENRLP
                     DTIAKDPGIGFRAAGDSSKMIVAGDGNLIESQVSKKGAIYPLGYDRFSQQQFGNRNFI
                     LNAADYLCDDTGVLALRAKEFRLRLLDAGKLNEQGTIIRWANVVLPLLLLWIAGWLFH
                     LGRRRRYQRK"
     gene            876..1898
                     /locus_tag="P5292_01850"
     CDS             876..1898
                     /locus_tag="P5292_01850"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRS37898.1"
                     /translation="MKNKILFLAAVLLAITAVWFGWSNKNSTIDKEELRFAVDTGSVR
                     KIFLADRTGRSITIERAGGATWLVNDSFPARQDLLKSLLTAIAGVEVKTRVSKAGFNN
                     VVKALAADGVKCEIYSGDSEQPVMTYYVGGPTADQLGTFMMKEGADLPFICELPGFNG
                     YLSPRYTANSLEWRDKVIYSLQPEEITKVRVNYPSRPDWSYSLSRTSNGFVLGDPLDG
                     TSIGIADTTSVRNYLYLFRKVPFETEAMKMRKEEQDSLSRLTPVAELEVTDTAGRVHG
                     LRLFPMPVQTGALATHDSLGNPLQHDADRFYATDPVQLKWWVLQHFTFDPLLRKRSDF
                     LSGGKK"
     gene            2092..3216
                     /gene="dnaN"
                     /locus_tag="P5292_01855"
     CDS             2092..3216
                     /gene="dnaN"
                     /locus_tag="P5292_01855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRS37899.1"
                     /translation="MKFIVSSSTLLKQLQQISGVLNSSNPLPILDNFLFIVEKGNLTI
                     SASDLESTMTTEVTLIEGKQSGKIAIPAKILLETLKTFSEHPLTFLIDDKTKGIEIVS
                     DNGKYKLTGFDGDEFPKVPALDGAKSIEVPASLLQKAIAKTLFATGNDELRPVMSGVF
                     SQFSKDSLTFVATDAHKLVRYRRTDHRAKQEGAFILPKKPLNLLKGILGTGDEPVSIA
                     YNDSNAFFTFGNFSLICRLIDGRYPNYEAVIPVDNPNKLTIDRIQFLQSVRRVSIFSN
                     KTTHQVRLKIQGSALQISAEDLDFANEANERLSCQYTGEDMEIGFNARFLVEMLSNLD
                     SEEVMMELSAPNRAGLLIPSGTESSGEDLLMLVMPVMLNN"
     gene            complement(3282..3425)
                     /locus_tag="P5292_01860"
     CDS             complement(3282..3425)
                     /locus_tag="P5292_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37900.1"
                     /translation="MQRLSTFNRLFLVFFILLFAFIIWKVPHEPKQSESGGTISTTLS
                     ADE"
     gene            complement(3437..3688)
                     /locus_tag="P5292_01865"
     CDS             complement(3437..3688)
                     /locus_tag="P5292_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37901.1"
                     /translation="MSIREMQNLIYQQGNFTFSDGRKDEGMVISRYNIKEARVEFYFI
                     PAKNLLAFESARSHHDLEAHKKMGQLVDVQSIVTATTIH"
     gene            complement(3892..5610)
                     /locus_tag="P5292_01870"
     CDS             complement(3892..5610)
                     /locus_tag="P5292_01870"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37902.1"
                     /translation="MKKFTLALFLIALNLTAALWARAEGIQPASQLAATATPGPITGP
                     TAVCANQTGVAYAVAALPGSVTYDWTLPIGASIVSGAGTASIVVDFGSDFGDICVTAD
                     DGSGASSPSCITTIAATGRPNSPDSIVGTVYSVCPGQVVTYSVLTPDPNAASYDWIVP
                     PTMTILSGQGTATITVEAGPAFSWGYLRVSASNCRGTTGQKVITVFNAPTKPGAISGP
                     PTGACPNGTYTYSIQAVPGATSYTWFAPAGCVIASPVTSGNPLTTSVTSVDITFPAGF
                     VNGSIYVQSNSGCNSSERRELKIRSVPVKPGGIRGPLFGVCDMTSVMYYVDSVPGAES
                     YTWTFTPGTYTTINGNGNDTIYVDFATGFTQATLCVTANNSCGSSVARCGVVFARPQT
                     PRFIDGPTGACNSNPATSIAYYEVQPMFGANGYDWSVPPGANITLGQGTTRITVDFLG
                     ASTGNVSVRAFNDCGISPARTLEVIVNPCRTAGSESNTEFLELIAFPNPAKNSIQIAF
                     QSTVAQDYGLKVVDITGRDVMSLNRSSSIGSNRELLDLTGMSPGIYLICLNSAGSQEK
                     IRVIVE"
BASE COUNT         1432 a         1516 c         1580 g         1376 t
ORIGIN      
        1 tgcaggcgga gatggacagc cttggccaga ccggcacgac tgtggcaatc ccgtacgagc
       61 ttaacctcga cgaccagcta ttccgctacg gagctcgcgt caataccgat ctggtcatgg
      121 accttcaggc ggcacccatc ccggtggtga ccgggtatgt gggtaatcaa cccaagcagg
      181 aattactacc ctggtattat tttcccctgt tggaaagcac ggataaccat ccgatcgtcc
      241 gcaacctgaa cgcagttaaa acggaattcc cttcctccgt cgacaccatt gatgtaaccg
      301 ggatcaagaa aaccgttttg ctggcgggca gtcagtattg ccgtctgcag ttgccgcccg
      361 cccgtatctc catgaatatc ctgcaacagg agccggatcc cgcgaccttt ccacgccggc
      421 atgtgccgat cgtcttgctc ctggaaggta actttcgttc cctttatgaa aaccggttac
      481 cggacacgat cgcgaaggat cccggaatcg gtttccgagc cgcaggagac agcagtaaga
      541 tgatcgttgc gggtgacgga aatctgatcg aaagtcaggt aagtaaaaaa ggcgctattt
      601 atccgctcgg atacgaccgg ttttcgcagc aacaattcgg caatcggaat ttcatcctga
      661 atgctgccga ttacctgtgt gacgataccg gagtgctcgc acttcgtgcc aaggaattcc
      721 ggctgcgttt gctggatgcc ggtaagttga acgagcaggg aacgatcatc cgctgggcca
      781 atgtcgtgct gcctttgctc ctgctttgga tcgcgggctg gctgttccac ctcggtcggc
      841 gtcgccgtta tcaacgcaaa taaggcgggt caaacatgaa aaacaaaatc ctcttccttg
      901 cagctgtcct tttagcgatt accgcggttt ggttcggatg gtcgaataag aacagtacga
      961 tcgacaagga agaactccga ttcgcagtcg atacgggaag tgtccggaaa attttcctcg
     1021 ccgatcgtac cggaaggtcc attaccatcg aacgcgccgg tggagccacc tggttggtga
     1081 atgattcatt ccccgctcgt caggacctct tgaaaagcct gttaacggct atcgccggcg
     1141 tggaagtcaa gacccgtgtt tcgaaagccg ggttcaacaa tgtggtgaaa gcgttggctg
     1201 ccgacggcgt caaatgcgag atctattccg gcgattcgga gcaacctgta atgacgtatt
     1261 atgtcggagg gccgactgcg gaccaactgg gcaccttcat gatgaaagaa ggcgctgacc
     1321 tgccattcat ctgtgaacta ccgggcttca acggttatct cagcccccgg tatacagcta
     1381 attcgcttga atggcgcgac aaagtgatct actcgttgca accggaagag atcacgaagg
     1441 tccgcgtgaa ttacccgtcc cgaccagact ggtcgtattc gctgagccgt acatccaacg
     1501 gattcgttct cggtgatcca cttgatggaa cttcgatcgg cattgccgac acgacttcgg
     1561 tcaggaacta tctctacctg tttcgaaaag tacccttcga gacagaagcg atgaagatgc
     1621 gaaaagaaga acaagattca ctctcccgac tgacgccggt tgctgagttg gaagtcaccg
     1681 ataccgctgg acgggttcac ggcttgaggt tgttcccgat gcccgttcaa accggtgcgc
     1741 tggcaacaca tgactccctg ggaaacccac tccagcacga tgcagatcgc ttttatgcaa
     1801 ccgatcctgt tcagttgaag tggtgggtgc tgcaacattt cacctttgat ccgctgcttc
     1861 ggaagagaag tgacttttta tcaggaggaa agaaataaac agtccggaag aaaccctttc
     1921 cgcgccctcg taaatagctg tttattaaca acatagcatt actctaggta gtatcgcttg
     1981 ttcatatttt aaggagcaat cacgctgtca ggaactcaga ttttacgggg aatgtggtag
     2041 gtttgtgggt cgaaggcgac ggtagttcgc cttttgtttc cttccgaacc tatgaaattc
     2101 atcgtatcga gcagtacgct cctgaagcag ttacaacaga tctccggagt cttgaactcc
     2161 agtaacccgc ttcccatcct ggacaatttc cttttcattg ttgagaaagg caacctgacc
     2221 atttccgctt cggatcttga gtcaacgatg accaccgagg tcaccctgat cgaagggaag
     2281 caatccggaa agatcgccat ccccgccaag atcctgctgg agacactcaa gactttttcg
     2341 gaacatcccc tcaccttcct gatcgacgat aagacgaaag ggattgaaat cgtcagcgac
     2401 aacggaaaat acaagttgac cggtttcgat ggtgacgagt tcccgaaagt tccggccttg
     2461 gacggcgcca agagtatcga agttccggct tcgttgttgc agaaggccat tgccaagacc
     2521 ctcttcgcga ccggcaacga tgaactgcgg cctgtgatgt cgggggtgtt ctctcaattc
     2581 tccaaagaca gtctcacctt tgtagctacc gatgcgcaca agttggtgcg ataccggcgt
     2641 actgaccatc gtgccaagca ggaaggggct ttcattttgc caaagaagcc gctcaatctc
     2701 ttgaagggta ttctgggtac gggtgacgaa cctgtctcca tcgcctacaa cgacagcaat
     2761 gcattcttca cgttcggcaa cttctcgctc atttgcaggt tgatcgacgg acggtatccg
     2821 aattacgaag cggttattcc ggttgacaat ccgaacaaac taacgatcga tcgcatccag
     2881 ttccttcaat ccgtgcgacg ggtctcgatc ttttcgaaca agactaccca ccaggtacgg
     2941 ctcaagatcc aggggagcgc gttgcaaatc agtgcggagg accttgattt cgccaatgag
     3001 gcgaacgaac gcctgagctg ccaatacacg ggtgaagaca tggagatcgg attcaacgcc
     3061 cgcttcctcg tcgagatgtt atccaacctc gacagcgagg aagtgatgat ggaactttcg
     3121 gcacccaacc gtgcaggact tttgattccg tccggcaccg aaagttcggg cgaagacctg
     3181 ctcatgctgg tgatgccggt tatgttgaac aactgatcaa acccggaaaa atgaaaacgg
     3241 cagcccgata aggctgccgt ttttgcttgt tcaggatcgg atcattcgtc cgccgaaagc
     3301 gtagtcgaga tcgtgccgcc gctttctgat tgcttcggtt catgcggcac cttccaaatg
     3361 atgaaggcga acagcaggat aaagaaaacc agaaacagtc ggttgaaagt gctcaaacgt
     3421 tgcatgactg tggatttcag tgaatcgtag tggcagtaac gatcgattgt acgtccacca
     3481 attgacccat ttttttgtga gcttccaggt catggtgcga gcgtgccgat tcgaatgcca
     3541 ggaggttctt cgcagggata aagtagaatt ctacccgggc ttctttgatg ttgtaacggg
     3601 agatgaccat cccctcgtct ttgcgtccat cggaaaaagt gaaattgcct tgttggtaaa
     3661 tcaggttttg catttcgcgg atactcatat cgacaggtgt ttttagttga ttgttgaaca
     3721 ataatacctt cagcacctgc cgggctgcaa tttactttgg taaacggagc ataattcagg
     3781 cctgtttatc gcacattccg gggtgaatgg accgttttgt acggtaacag aaaaagtaaa
     3841 agggccttgg aagttgtacc tccaaggccc tttttatcaa aaattctgta attactctac
     3901 aattacccgg attttctcct gcgatcctgc cgaattgagg cagatgaggt agataccagg
     3961 gctcatgccg gtcagatcca acagttcacg gtttgaaccg attgagcttg aacgattcag
     4021 cgacatgacg tcgcggccgg tgatgtcgac cactttcaaa ccgtaatcct gagcaacggt
     4081 agattggaag gcgatctgta tggaattctt cgccggattc gggaacgcga tcagctccag
     4141 gaattcggta ttgctctcag aaccagcagt acggcaagga ttgacgatca cttccagcgt
     4201 gcgggccggg ctgatgccac agtcgttgaa tgcgcgcaca cttacatttc cggtcgaagc
     4261 acccaaaaag tctacggtaa tgcgcgtagt tccttgtccg agtgtgatgt tggcacccgg
     4321 cggtacactc cagtcatatc cgttcgcgcc gaacatcggt tgtacctcgt aatatgcaat
     4381 gctggtcgcc ggattggaat tgcatgcgcc ggtcggtcca tcgatgaagc gaggcgtttg
     4441 cggacgcgcg aataccactc cacaacgagc cactgaacta ccacaggaat tgttcgccgt
     4501 cacgcagagc gtggcttggg tgaagccggt agcgaaatcc acgtagatgg tatcgttccc
     4561 gtttccattg atcgtcgtgt aagttccggg ggtaaacgtc caggtgtacg actccgcgcc
     4621 cggcacagaa tcgacatagt acattacgct cgtcatatca cagacgccaa agagtggacc
     4681 acggattcca cccggcttga ccggtacaga acggatcttc agttcgcgtc gctcggaaga
     4741 gttacaaccg ctgttcgatt gcacgtagat cgagccgttc acaaaacctg ccgggaaagt
     4801 aatatccacg gaggtgacag aggtagtcaa cgggttaccg ctcgtcaccg gggaggcaat
     4861 gacacaacct gccggtgcga accaggtgta cgaggttgcg ccggggaccg cctggatcga
     4921 atacgtatag gtaccgttcg gacaggcgcc ggtcggcggg ccggaaatag cgcctggttt
     4981 ggtcggtgca ttgaatacgg tgatcacttt ttgtccggtg gttccacgac agttgctcgc
     5041 cgagacacgc aagtaccccc agctgaaagc gggacccgct tctaccgtga tggtagcagt
     5101 tccttgcccg ctgaggatgg tcatggtcgg tggaacgatc cagtcgtacg aagcagcatt
     5161 gggatcagga gtcaggacgg aatacgtcac gacttgaccg ggacatacgc tgtaaacagt
     5221 gcccacgatc gaatccggag aattcggtcg gccggtagct gcgatggtgg tgatgcagga
     5281 cgggctactg gctccgcttc cgtcatcggc cgtaacgcag atgtcgccga aatcgcttcc
     5341 gaaatcaacg acaattgatg ctgtaccggc accagagacg atggatgcac caatgggtag
     5401 tgtccagtcg taggtaacgc ttccgggtaa tgccgctacg gcatacgcta caccggtttg
     5461 gttggcgcac actgctgtcg gacctgtgat cggtccgggt gtcgcagttg ctgcaagttg
     5521 cgatgcgggt tgaatgcctt cggcacgcgc ccacagcgct gctgtcagat taagcgctat
     5581 cagaaataac gccagggtaa atttcttcat gaccgtagaa attgagattt gattccgact
     5641 gttatatagt ccggaagtcg ccctacagtt gaggtgtcag gctgctgcga cttacaactg
     5701 taaggttcat ggaagaaatg tcgccgcatt tcgaaatcac ggtgaagagt atccaacaat
     5761 cttgcgatac tggcatgaaa gcgagtcaat gggagttatc gcctccaaac ttcatttgaa
     5821 aagaagccga tagaatcgca tggatagaaa aatgaaggat aagaaaagca caatcgagcg
     5881 gtaaatagcg ctcatgttta caga
//
LOCUS       DAOTON010000054         5839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_11642_length_5839_cov_3.567082, whole genome shotgun sequence.
ACCESSION   DAOTON010000054 DAOTON010000000
VERSION     DAOTON010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11642_length_5839_cov_3.567082"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="P5292_01875"
     CDS             complement(<1..603)
                     /locus_tag="P5292_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37903.1"
                     /translation="MKRKQFLQSLAASATIPSLINGFSFRSFAASPLLQSLAASTIDD
                     HVLVLVQLTGGNDGLNTVIPLDQYANLSLARPNILIPDTAVLSLNGITGTGLHPAMGG
                     MQNLFNDGKLAIVQGVSYPSPNFSHFRATDIWLTASDSSQVLNTGWLGRYLNYEYPNF
                     PTGYPNPTMPDPLALQIGTSVSLALQGPTVGMGMTITDPTN"
     gene            complement(615..2267)
                     /locus_tag="P5292_01880"
     CDS             complement(615..2267)
                     /locus_tag="P5292_01880"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRS37904.1"
                     /translation="MNRRDFLSGRPQTDEATLVEPHARTQTGLAPYSGAWSLSEVKHL
                     LRRTRFGATKAECDHYLSIGLSGALNELLTPLATQPSPPLYTATAVYNDPNVPFGQTW
                     VNAAYDALANGQRLRSFRSWWIGLMLQPDPGITERMVLFWHNHFVTEAATLGNAVMGY
                     QNNALLRLHALGNFKTMTRAVTIDPGMLRYLNGYLNTASAPDENYGRELQELFTVGKD
                     ATGQPYYTEDDVKNAAKVLTGYRITFSNGTAYFDPNRHDTSDKVFSAYYGNAVIAGRS
                     GTAGQDELDDLLDMIFSRQEVALLIARKLYRYFVYYEIDAATETDVIQPLADIFRNNN
                     YEIIPALQALLGSEHFFDVANRGALIKTPIDFAVSHARDFAVAFPDASALEDQYALWY
                     RIFSQASAMGQSIGDPPDVAGWPAYYSAPAYHELWINATTLPARNAFTDRMVSTGYTS
                     GSSNISFDVVAYTETLADATDPNLLIQEVLDRHYCFDPSQTLKDYLKSFLLSGQAQDH
                     YWTDAWDDYLADPTNTMYYGIVESRLQGMYQYLMNLAEYQLS"
     assembly_gap    2406..2505
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2506..2946
                     /locus_tag="P5292_01885"
     CDS             <2506..2946
                     /locus_tag="P5292_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37905.1"
                     /translation="VIGFSSMQKFHPISIGTFYFAPPELEISVDHYSNVLGPSGAGWG
                     DDETLLPICWKTSCRQYQTDCRIRFMMILHWIQSAPEGPNTFVDGKPTTPKLRRSVTT
                     GPSGQQQFLSSVIGFSSMQKFHPISIGTFCFAPPELAISVNPLF"
     gene            3163..3705
                     /gene="tnpA"
                     /locus_tag="P5292_01890"
     CDS             3163..3705
                     /gene="tnpA"
                     /locus_tag="P5292_01890"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRS37906.1"
                     /translation="MKHLEFFCYLNKISRIYAVATDRITLLLCSMADSFTQVYLQLVF
                     STYRRQPFIAQEWKDELHKYITGILKNQQQVPIRINSMPDHIHILFGMKPQRSMSEVI
                     REIKADSTNWVKKNRWANGKFRWQNGYALFSYSQSHLDRVVKYIERQEYHHRSMKYED
                     EYTRILDLYKIVRIGKKVFD"
     gene            complement(3714..5540)
                     /locus_tag="P5292_01895"
     CDS             complement(3714..5540)
                     /locus_tag="P5292_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS37907.1"
                     /translation="MKNYLRLLRLVTGYKPYALLNILCNVLSVIFSLFSLTMVAPFLN
                     VLFSQGQTYTEMPWAFSVKVLLNNFNYHLSQYVAEHGKLEALSVISGLVVVMFLLKNL
                     FRYLGMYFMAPIRTGVVRDLRNELYGKVLVLPLSYFSNERKGDIMARMTNDVQEIEWS
                     IMQSLEVIFREPLTVILFLTTMVLMSPQLSLFVFVLLPIAGILIGQIGKSLRRTSGKS
                     QEQMGNLLSNIEETLGGLRIIRAFTAEEFSFEKFRENNNRFRKLQKRLYRKRDLSSPL
                     SEFLGAVVLVIVMYFGGRLVLSADATLDPSVFIAFIAIFSQLIPPAKAFTEAYSNVQR
                     GLASAERINKILDAEVTIRNRPGAVAIESFKDRIAYRDVSFAYHQGGTGWVLKHISLE
                     VPRGKTIALVGQSGSGKTTLVDLLPRFYELEQGSITIDGQDIRSLTLESLRGLMGIVN
                     QESILFNDTVFNNIAFGMPDATLEDVTRAATIAHAHEFIAHLPEGYQTNIGDRGGKLS
                     GGQRQRIAIARAILKNPPILILDEATSALDTESERLVQDAITRLMENRTVLVIAHRLS
                     TIQHADEILVMQQGEIIERGRHADLLARGGVYKKLYDLQVFA"
BASE COUNT         1347 a         1473 c         1619 g         1300 t
ORIGIN      
        1 gttggtcggg tcggtgattg tcatgcccat tccgaccgtt ggtccctgca aagccaggga
       61 aacactggtt ccgatctgca aggccagtgg atccggcatg gtcggattcg ggtagccggt
      121 ggggaagttg ggatactcgt aattgaggta gcgacccagc caaccggtat tgagcacctg
      181 gctgctgtcg gatgcggtca accagatatc cgtcgcacga aagtgcgaga agttgggaga
      241 cggataactc acgccttgca cgatggccag ctttccgtca ttgaacagat tctgcatccc
      301 gcccatggcc ggatgaagtc cggttcctgt gatcccgttg agtgagagga ccgccgtatc
      361 cgggatgagg atgttcggtc gtgccagcga aaggttggca tactgatcga gcggaatgac
      421 cgtgttcaaa ccgtcgttgc cgccggtgag ttgaaccagc acgaggacgt ggtcgtcgat
      481 cgtcgatgcc gccagcgact gcaacaaagg cgatgctgcg aaggaacgga acgagaatcc
      541 gttgatcagc gaaggaatgg tggccgatgc agccagcgat tggagaaact gttttctttt
      601 catggccagc gctgtcagga aagttggtat tcggcaaggt tcatcaggta ctggtacatc
      661 ccctgtaaac gggattcgac aatcccgtaa tacatcgtat tggtcggatc cgccaggtag
      721 tcgtcccagg catcggtcca gtaatggtcc tgagcctgtc ctgataaaag gaagcttttg
      781 agatagtctt tcaacgtctg ggacggatca aagcagtaat gccgatcgag tacttcctga
      841 atgagcaggt tggggtcggt tgcatccgcg agtgtttcgg tataggctac gacatcgaag
      901 gaaatattgc tgctgcccga cgtataaccg gtggacacca tgcggtcggt gaatgcattg
      961 cgcgccggga gtgtggtcgc gttgatccac aattcatgat acgccggagc ggaatagtaa
     1021 gccggccaac cggccacatc gggcgggtct ccgatcgact gccccatggc gctggcctgc
     1081 gagaaaatcc ggtaccagag tgcatactgg tcttccaatg cggaagcatc cggaaaggcg
     1141 actgcaaaat cgcgcgcgtg ggataccgcg aaatcgatgg gcgttttgat gagcgcaccg
     1201 cgattggcta catcgaaaaa atgttcactg ccgagcagcg cctgcaaagc cgggatgatc
     1261 tcgtaattgt tattccgaaa gatatcggcc agcggctgta tgacatccgt ctcggtcgcg
     1321 gcgtcaatct cgtagtagac gaaatagcgg tacagtttcc gcgcgatcag caaggcaacc
     1381 tcctgccgtg agaagatcat gtcgagcaga tcgtcgagtt catcctgacc ggcggtacca
     1441 ctgcgaccgg cgatgaccgc attaccgtaa taggcggaaa agaccttgtc gctggtatcg
     1501 tggcgattcg ggtcgaaata agctgttcca ttcgagaacg tgatgcggta tccagtgagc
     1561 accttcgcgg cgttcttcac atcatcctcc gtataataag gctgaccggt cgcatctttg
     1621 cctaccgtaa acaactcctg aagttcgcgt ccataatttt catccggcgc gctggcggta
     1681 ttcaggtagc cgttcaggta gcgcaacatt cccggatcga tggtaacggc ccgggtcatg
     1741 gtcttgaaat tccccaaggc atggagtcgc aaaagtgcgt tgttctggta gcccattaca
     1801 gcgttcccca gcgtggccgc ttcggtcaca aagtgattgt gccagaataa gaccattcgt
     1861 tccgtgatgc ccggatccgg ttgcagcatc aagccgatcc accaggaacg gaacgagcgc
     1921 aggcgttgtc cgttcgccaa ggcatcgtaa gcggcgttta cccacgtttg cccgaagggc
     1981 acattcgggt cgttgtaaac cgcggtagca gtatataaag gtggtgaggg ttgcgttgcc
     2041 aggggtgtga gcagttcgtt caaagcgcca ctcaagccaa tggacagata atgatcacac
     2101 tcggccttcg ttgctccgaa acgggtgcgg cgaagcaggt gcttcacttc gctcaggctc
     2161 caggcaccgg agtaaggcgc aagcccggtt tgcgtgcggg catgcggttc gacaagtgtt
     2221 gcttcgtcgg tttgcggccg accggaaagg aaatcacgac ggttcatgcg agtgggcgaa
     2281 tgttccctaa gacccggaca aatgggcaag gtttaaggct tgaatgaaag gacgacaggg
     2341 atgttagcgg aaggatgaag ataatgcaca gcggacagat tggatccaat gatcgattcg
     2401 ggtttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtgat tggattttca
     2521 tcgatgcaga aatttcaccc gatctcaatc ggcacctttt atttcgctcc tccggagctt
     2581 gaaatttctg tggatcatta ttccaacgtg ttgggcccct ccggggctgg gtggggtgat
     2641 gacgaaacgc ttttgcccat ttgttggaag acatcatgta ggcaatatca aacggactgc
     2701 cgtattcggt tcatgatgat ccttcactgg atacaatcag ccccggaggg gccaaatacg
     2761 tttgtcgatg gcaaacccac tactccaaag ctccggagga gcgtaacaac aggcccttca
     2821 gggcaacaac aattcttgag ttccgtgatt ggattttcat cgatgcagaa atttcacccg
     2881 atctcaatcg gcaccttttg tttcgctcct ccggagcttg caatttctgt gaatccatta
     2941 ttctaacgtg ttgggcccct ccggggctgg gtggggtcag gttgtaacgc atatcagttc
     3001 attagaatag ataacagtta gaaatttcga ctcaactcac attttttcat cttgaccctt
     3061 tcttcggaca caatttgccc cggagggact caacattatg gtagttagga ttcctataat
     3121 ctaaatattc cattggagcg atcgctcaac ggaatcggaa gaatgaagca tttagaattc
     3181 ttctgctacc tgaataaaat atcccgcatt tatgcggttg caactgaccg catcacccta
     3241 ttgctttgca gtatggcaga ctcctttaca caggtttact tacaacttgt attttccacc
     3301 taccgcaggc aaccattcat cgcgcaggaa tggaaggacg aacttcataa gtacatcaca
     3361 ggtattctga agaatcaaca acaagtgccc atcaggatca acagcatgcc cgatcatatc
     3421 catatcctct tcggcatgaa gccacagcga tccatgtcag aggtgatccg ggaaataaaa
     3481 gccgactcga ccaactgggt aaagaaaaac cgatgggcga atgggaaatt tcgatggcag
     3541 aacggctatg cgctcttctc ctactctcag tctcatctgg atcgtgtcgt caagtacatt
     3601 gagcgacaag aatatcacca tcgttccatg aaatatgaag atgagtacac ccggattctg
     3661 gatctgtaca aaatagttcg aatcggaaaa aaagtcttcg actgactgaa ggatcaggcg
     3721 aaaacctgca gatcatacaa cttcttgtag acgccgcccc tggccagcag atcggcgtgg
     3781 cgaccgcgtt cgatgatctc gccctgttgc atgaccagta tctcgtcggc gtgttggatg
     3841 gtggaaaggc ggtgggcaat gaccagtacg gtgcggttct ccatcaggcg ggtgatcgcg
     3901 tcctgcacca gccgctcgct ctcggtatcg agcgccgagg tggcttcgtc gagaatgagg
     3961 atcggcgggt tcttcaggat cgcacgggcg atcgcgatac gctgccgctg gccaccgctg
     4021 agctttcctc cgcgatcacc gatattggtc tggtaacctt ccggcaaatg cgctatgaac
     4081 tcgtgggcat gtgcaatggt ggcggcacgc gtgacgtctt cgagcgtggc atccggcatg
     4141 ccaaaggcga tgttgttgaa taccgtgtcg ttgaacagga tgctctcctg gttcacaatg
     4201 cccatcagtc cgcggaggct ctcgagcgtg agtgaacgga tgtcctgtcc gtcgatggtg
     4261 atacttccct gctccaactc atagaagcgc ggcagcagat cgaccagcgt ggtcttgccg
     4321 gatccgcttt gtccgaccag cgcgatcgtt ttgccgcgcg gcacctccag gctgatgtgc
     4381 ttcaacaccc agccggtacc gccctggtga taggcgaaac tcacatcccg gtaggcgatg
     4441 cggtccttga aggactcgat ggcaacggca cccggacggt tacggatcgt gacttccgcg
     4501 tcgaggatct tgttgatgcg ttcggccgag gcgagtcctc gttgcacgtt gctataagct
     4561 tccgtaaatg ccttggctgg cgggatcaat tgggaaaaga tggcgataaa cgcgatgaac
     4621 acactcggat caagtgtggc gtcagcactc aacaccagtc gaccgccgaa gtacatgaca
     4681 atgaccaata cgaccgcgcc gaggaactcc gacaagggag aactcaggtc gcgtttccgg
     4741 tagaggcgtt tctgtaactt ccggaagcgg ttgttgttct cgcggaactt ttcgaagcta
     4801 aactcctccg cggtaaacgc acggatgatg cgcaatccgc ccagcgtctc ttcgatgttg
     4861 ctcagcaggt tgcccatctg ctcctgcgat ttgcccgatg tccgccgcag gcttttcccg
     4921 atctgaccga tgaggatgcc ggcgatgggt aataacacaa aaacaaagag cgatagttgt
     4981 ggactcatca gcaccatggt ggtgaggaag aggataacgg taaggggctc gcggaagatc
     5041 acctccaagg actgcatgat gctccattcg atctcctgca cgtcgttggt catgcgcgcc
     5101 atgatgtcgc cttttcgttc gttggagaaa tacgagagcg gcagtaccag gactttccca
     5161 tacagctcgt tgcgcagatc gcgcaccacg ccggtacgga tcggcgccat gaagtacatg
     5221 ccgaggtaac ggaacaagtt cttcaacagg aacatcacca cgaccagtcc agagatcaca
     5281 ctcagggctt cgagcttacc gtgctcggcc acatactggc tgaggtgata gttgaaattg
     5341 ttgagcagga ccttcaccga aaaagcccag ggcatctcgg tgtaagtttg accctgactg
     5401 aagagtacgt tcaaaaaggg cgctaccatt gtcagcgaaa agagcgaaaa gatcaccgac
     5461 aacacattgc acaggatatt cagcagtgca tacggtttgt acccggtgac cagccgcagg
     5521 agccgaaggt agtttttcat ggggcggggt aaaggtagga aagaagggtg tcgggttttg
     5581 agttccgggt tccagactaa agggacgagg gacgagaccg agtgacgagg aggtttttat
     5641 gtttagagtt tcctgcctct gcatccgcta cggcagtcag ccgactaagg tcgctttttt
     5701 agaacgggac gagggccgat ggaattgaac cgaggctatt ttttcaacct tgtaacattc
     5761 aaccttcaac caaccgggaa cccggaactc gaaacccgga acaaccaact ggcgcatgcc
     5821 cggcctgccc tgtcccggg
//
LOCUS       DAOTON010000055         5788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_11763_length_5788_cov_3.530787, whole genome shotgun sequence.
ACCESSION   DAOTON010000055 DAOTON010000000
VERSION     DAOTON010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11763_length_5788_cov_3.530787"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..2628
                     /locus_tag="P5292_01900"
     CDS             220..2628
                     /locus_tag="P5292_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS37908.1"
                     /translation="MLQHPLQILYIEDDDLDRMIMKRALNSSGLPVELRFAENIDAGR
                     EATQGVEYDCIFLDYNLPGGTGLELLKVIRASGNNSPIIIVTSQGDEKIAVEAMKNGA
                     NDYIPKSFLSADGLSQSLRFVLRFRDQERERIRLQEELNAAQQQLQAVASNSPIILFA
                     FDQNQQLALLEGRSQTLFGIAPEAVLHQGLIEVQSKLPMNAEDVTTAMHGKEVTTVVA
                     FNDRYFEIYYAPVRDASQQISGVMGIAADITTHKAAEDQLLQAKQMAEETSRMKEQFL
                     ANMSHEIRTPMNGIIGLTRILLETSLTEEQERYLQSIKTCSNNLLVIINDILDFSKIE
                     AGKMNFEQVPFRMNDIVAHTIELFQTKADEKSIRLVQEIDSQVPMALIGDPTRLSQIL
                     NNLVSNAIKFTDKGDVTVRLSLRSKRDQDVTVDFEVRDSGIGIPEKSLDSIFESFTQA
                     SSDTTRKFGGTGLGLTIVKKLIELQGGAITVRSKVGQGTTFAFHISFPIATEASFRPV
                     EMIQEDKLDISHLRILIAEDNPVNQLIVKKLFKDWKTEVQFADNGKLAIEQLQQANFD
                     LILMDIQMPEMDGNTAAHYIRTELPEPFRSIPIMAMTAHATQTERDKSFQVGMNEYIS
                     KPFDPAELRKKILAITQDRKTPLNQAAEPAATTTTVLLPESNNNGNVTKNPASPEQVQ
                     AILSEHKINLTYLKSIADGNDGFIIEMIEMFLNKTPEALEKMEACFREKNWEELRQIA
                     HRIKPSFGYVGLATTQQMLAEIEKLSEDPSGDPSRVGTLMGEVRQISRSAFSQLEQEL
                     GSMR"
     gene            2743..3108
                     /locus_tag="P5292_01905"
     CDS             2743..3108
                     /locus_tag="P5292_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS37909.1"
                     /translation="MKILVCEDDEMVLKMVEFKLRKDGYEVQLAGDGKAALDQIRSFQ
                     PDLIITDIMMPYLTGLEIVHQVRKTLNLATPIIIVSSIGLEKTVLEAFQLGADDFITK
                     PFSPNELSVRVKKLLMKTA"
     gene            3248..4507
                     /locus_tag="P5292_01910"
     CDS             3248..4507
                     /locus_tag="P5292_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HRS37910.1"
                     /translation="MLLAFGGKLSAQSKGDTAIVINERTDIDALFKQAREVSFAGNNL
                     QARRILQRILERKPDYYDVRTFLGRTYAWDRQYDNARTELSRVLIDRENDQDALLALI
                     DVEDWSGNPEVANQYLKLGLSYYPTSEALLMKKARLQIKADDKESAAITLRRILDFNP
                     GNKEAIDLMNSLNTARLNNRVQISYTVDFFDQKTSPWQFISADIGRSFKFGSVIMRGN
                     VAERFGNRGLQYEVESFLRLFKGNYVNAAMAFSTADQVFPGQRYSAEMYQKLPWGFEL
                     SGGLRYLEFTDGTTIYTGSLGNYFRDYWIAFRAFITPKPDVVTSENSFFARTSQTLIL
                     TMRKYMGDADNFIGLRAGRGDSPDERRVIDATTPRLRSWSGDLEVQRRAFGRWIVRAD
                     MGYAYEEIRQDTYQQRITLGLQLRTNF"
     gene            4516..>5788
                     /locus_tag="P5292_01915"
     CDS             4516..>5788
                     /locus_tag="P5292_01915"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRS37911.1"
                     /translation="MHRFRQIIALLFLGFVLFSGQVLSQTPAKAPAKVKEDPRVTARR
                     FFEKDTIHTIRIYFPQCNYWDSLTYYKKIYDSLEVSTYMLATVIIDGKKMYSCGVRFK
                     GESSYEFYPGKKKPFRIKFNKFDKKQDYQGIEEINLNNNFKDPTMMREKLYLDLMNKY
                     DLPAPRSSYAKVYLNDKYWGLYTLTEPIDNEFLKSRFRNEDGNLYQGEPVASMAFLGN
                     DPTKYWNRYVKKNNLKQNDWTDLVKLIKLINDSTLSDAAYATQLDGILDTDKMLRAWA
                     INNLIGNIDAYNIFYPHNYFIYHDSLDGRWKWISLDGNYAFAAWNPIMNLPQLQRMSV
                     MVPDSTPYRDPRPLMERTIGKNSFYQKRYLNILKELVNSDFLPERMNAKIDSLSLRIR
                     VSVYADLNKMYSNADFDTNLESTLGDPLDPGN"
BASE COUNT         1495 a         1537 c         1453 g         1303 t
ORIGIN      
        1 ggcttgtaag tttggcgttg aaacgattat tgacctatac tgtttactgt ttactgttta
       61 ctattcacta ttcactattc gctattcgct gttcactccc caccctctcc ccacctattt
      121 cgaccttttg taaggatttc gggctttttt agaaaactac gggcaacttc tctggtacaa
      181 tgttacacgc cggcctatat ccggacgtga accattacca tgctgcaaca cccactccag
      241 atcctttaca tcgaggatga tgatcttgac cggatgatca tgaaacgtgc cctgaacagt
      301 tcgggcttgc cggtagaatt aagattcgcg gaaaacatcg atgcgggccg agaagccacg
      361 cagggggtcg aatacgactg tatttttctg gattataatc tacccggcgg tacgggtctg
      421 gaattgttga aagtgatccg tgcttcgggc aataactccc cgatcatcat cgtcacatct
      481 cagggtgatg agaagatcgc tgtggaagcc atgaaaaacg gcgccaacga ttatattccg
      541 aagagtttcc tgtctgccga cggcctctcg caaagtcttc gcttcgtact tcgattccgg
      601 gatcaggaaa gggaacgcat acggctgcag gaggaactca acgccgcgca gcagcagttg
      661 caggctgtag cttccaactc accgatcatc ctgtttgcat tcgaccagaa ccagcagctt
      721 gcgctgctgg aaggccgcag tcaaacgctt ttcggcattg ctccggaagc ggtactccac
      781 caggggctta tcgaagtcca aagcaagctg ccgatgaacg cggaagacgt cactaccgcg
      841 atgcacggaa aggaagtcac gacagtagtg gcattcaacg accgatattt cgagatctac
      901 tacgcgccgg tacgtgatgc cagtcagcag atctccggtg tcatgggaat cgcggcggac
      961 atcacgaccc acaaagcggc agaagaccaa ttgctacaag caaagcagat ggccgaggaa
     1021 acttcgcgca tgaaggaaca attcctcgcc aacatgagtc acgagatccg gaccccgatg
     1081 aacgggatca tcggcctgac caggatcctg ctggaaacat cgctcacaga agaacaagaa
     1141 cgctatctcc aatccatcaa gacctgctcg aacaatcttt tggtgatcat caacgacatc
     1201 ctggattttt caaagatcga agccggcaag atgaacttcg aacaagtgcc gttcaggatg
     1261 aacgacatcg ttgcgcatac gatcgagttg ttccagacca aggccgatga aaaatcgatc
     1321 cggctggtgc aggagatcga ctcgcaagtg ccgatggcgt tgatcggtga ccctacccga
     1381 ctgagtcaga tcctgaacaa cctggtcagc aatgccatca agttcaccga caagggcgac
     1441 gtgacggttc ggttgagcct tcggtcgaaa cgggaccagg atgtcacggt cgatttcgaa
     1501 gtccgggaca gcggaatcgg catccctgaa aagagcctcg acagcatctt tgagagcttc
     1561 acccaggcat cgagcgacac gacccgaaag ttcggcggta ccggtcttgg gcttaccatt
     1621 gtgaagaagc tcattgaatt gcagggcggc gctatcaccg tccgcagcaa ggtcggtcag
     1681 ggtaccacct tcgcttttca catcagtttc ccgatcgcga cggaagcttc cttccgtccg
     1741 gtggagatga tccaggaaga caagctggat atctcgcatc tgcgcattct gatcgcggaa
     1801 gacaatcccg tgaaccagtt gatcgtgaaa aagctgttca aagactggaa gaccgaagtt
     1861 caattcgccg acaacggcaa actggcgatt gagcaattgc agcaggcgaa tttcgacctg
     1921 atcctgatgg atatccaaat gcccgaaatg gatggaaaca cggcggctca ctacatccgc
     1981 accgagttac cggaaccttt ccggagcatc ccgatcatgg ccatgaccgc gcacgccacg
     2041 cagaccgaac gggacaaatc ctttcaggtc ggtatgaacg agtatatcag caagccgttt
     2101 gatccggcgg agcttagaaa aaagatcctg gctataaccc aggaccgtaa aactccgtta
     2161 aatcaagctg ccgaaccggc cgcaactacc actacggtgc tactcccaga atccaacaac
     2221 aacggaaacg tgacgaaaaa tccagccagc cctgaacagg tgcaggcaat cctgtccgag
     2281 cacaagatca acctcactta tctaaaatcg atcgctgacg gcaacgacgg gttcatcatt
     2341 gagatgatcg agatgttcct gaacaagacg ccggaagcgt tggagaagat ggaagcctgt
     2401 ttccgggaaa agaactggga ggaattgcgt cagatcgccc accgcatcaa accctcgttt
     2461 ggttatgtag ggttggcgac tacccagcag atgctggcgg agatcgagaa actcagtgaa
     2521 gacccgagtg gcgatccctc gcgtgtaggg accttgatgg gagaggttcg gcagatcagc
     2581 cggtccgcct tttcacaatt ggaacaggaa ctaggcagta tgcgctgaag ccgaagctta
     2641 tgcagtaatt actgaccttc ccctcttgaa aacttcaatg ccgctccgga atgttccggg
     2701 gcggtttttg cttttatcgc tatctttcgg cctcttagaa gaatgaagat cctcgtttgc
     2761 gaagacgatg aaatggtgct gaagatggtg gagttcaagc tccgaaagga tggctatgaa
     2821 gtccagttag caggagatgg gaaagccgcg cttgaccaga tccgcagctt tcaacctgac
     2881 cttatcatca ctgacatcat gatgccctac ctcaccggtc tggagatcgt ccaccaggtg
     2941 cggaagacgc tcaatcttgc cacgccaatc atcatcgttt cttctattgg cttggagaag
     3001 accgttttgg aagcgtttca gctaggcgcc gacgacttta tcaccaagcc attcagcccg
     3061 aatgagcttt ctgtaagggt gaaaaagtta ttgatgaaaa cggcctgatc cttgtttaac
     3121 ttttgcttcc gtattgcgta aagcaataag cggattctgt agttaatttc gcagtccgac
     3181 ttccggaatc cctgcctaaa aaatgcacaa tcgctttatt ttcagataca taaccctttt
     3241 ctgggttatg ctccttgctt tcggggggaa gctttccgct cagagcaaag gcgataccgc
     3301 gatcgtgatc aacgagcgta ccgacattga cgccctcttc aaacaggcca gggaagtcag
     3361 ttttgccgga aacaacctcc aggcaagacg tattttacaa cgtatcctgg aacgaaagcc
     3421 ggactattac gatgtccgca cctttctcgg tcgcacctat gcatgggatc gccagtatga
     3481 caacgcccgt accgaactca gccgggttct gatcgatcgg gaaaatgatc aggacgcgtt
     3541 gctggccctg atcgatgtgg aagactggtc gggtaatccc gaagtggcca accagtacct
     3601 gaagctcggc ctcagctact accctacatc ggaggctttg ctgatgaaga aagcccggtt
     3661 gcagatcaag gccgacgaca aggaaagcgc cgcgatcacc ctccgccgca tcctggattt
     3721 caatcccggt aataaggaag cgatcgatct gatgaacagt ctgaatacag cccgcttgaa
     3781 caaccgggtt cagatcagtt acaccgtcga tttctttgac cagaagactt ccccgtggca
     3841 attcatctcg gcggatatcg ggcgatcctt caagttcgga tcggtgatca tgcgcggaaa
     3901 cgttgccgag cgatttggca atcgtggctt gcaatatgag gtggagagct ttctccggct
     3961 attcaaaggg aactatgtga atgccgcgat ggctttttct acagccgatc aggtattccc
     4021 cggacaacgt tactcggccg agatgtacca aaagctaccg tggggtttcg agctatccgg
     4081 aggcttgcgc tatctggaat tcaccgatgg tacaaccatc tataccggca gtctgggcaa
     4141 ttacttccgc gattattgga tcgctttccg cgcgttcatc acacccaagc cggacgtagt
     4201 gacctccgag aacagtttct tcgccaggac ttcccagacc ctcattctga ccatgcgtaa
     4261 atacatgggc gatgccgaca acttcatcgg cttgcgtgca gggcgcgggg attcacctga
     4321 tgaacgacgg gtgatcgatg ccacgactcc ccgactacgt tcctggtccg gtgatctcga
     4381 ggtacagcgt cgggcattcg ggcgatggat tgtccgggcc gacatgggct atgcctacga
     4441 ggagatccgt caggacacct atcaacaacg tatcacactg ggactgcaat taagaaccaa
     4501 tttctaatcc gccacatgca tcgattccgg caaataatcg cgcttctttt tctcggtttc
     4561 gtccttttct ccggacaagt cctttcgcag acacctgcaa aagctcccgc gaaggtgaag
     4621 gaagatccgc gtgtaacagc ccgcaggttc tttgagaaag acacgataca caccattcgg
     4681 atctacttcc ctcagtgcaa ctattgggat tcgctgacct attacaagaa gatctacgac
     4741 tcgctggaag tttcaaccta tatgttggcc acggtcatca tcgacggtaa aaagatgtat
     4801 tcctgcggtg tgcgattcaa gggcgagtcc tcttatgaat tctatccggg taagaaaaag
     4861 ccctttcgaa tcaagtttaa caaattcgat aaaaagcaag attatcaggg aattgaggag
     4921 attaacttaa acaacaactt caaggatcca accatgatgc gggagaagtt gtacctcgac
     4981 ctgatgaaca aatatgacct gccggcacct cgctcctctt atgccaaggt ttacctgaac
     5041 gacaaatact ggggactgta caccctgacg gaaccgatcg ataacgagtt cctgaaaagc
     5101 cggttccgca atgaggatgg caacctgtat cagggcgaac cggtggccag tatggcattt
     5161 ctgggtaatg atccgaccaa atactggaac cggtacgtca agaagaacaa tctgaagcag
     5221 aatgactgga ccgacctggt caagctcatt aaactgatca acgactccac cctctcggat
     5281 gcggcctatg ccactcagtt ggacgggatc ctggatacgg ataagatgct gcgtgcctgg
     5341 gccatcaata accttatcgg gaatattgat gcctataaca tcttttatcc gcacaactac
     5401 ttcatttacc acgacagcct ggatggacgt tggaaatgga tcagtctgga tggcaactat
     5461 gcctttgccg cctggaaccc gatcatgaac ctgccccaac tccagcgtat gagcgtcatg
     5521 gtaccggatt cgacccccta ccgggatcct cgtccgctga tggagcgtac gatcggaaag
     5581 aacagcttct atcaaaaacg ctacctgaac atcctgaaag aactggtgaa ttccgatttt
     5641 cttccggaac ggatgaacgc gaaaatcgac agcttatcgc tccggatccg ggtttcggtc
     5701 tatgccgacc tgaacaagat gtactcaaat gcggacttcg acaccaacct ggaatcgacc
     5761 cttggcgatc cgttggaccc gggtaatt
//
LOCUS       DAOTON010000056         5787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_11765_length_5787_cov_3.030181, whole genome shotgun sequence.
ACCESSION   DAOTON010000056 DAOTON010000000
VERSION     DAOTON010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11765_length_5787_cov_3.030181"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="P5292_01920"
     CDS             complement(<1..576)
                     /locus_tag="P5292_01920"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRS37912.1"
                     /translation="MEPIDLEAADHDELYEHHRFVVDKGQAPLRVDKYLMNRLTGASR
                     NKIQQACDAGCILVNGKETKPSYKVKPLDQVQVVLPEPVREFELVPENIPLNIVFEDE
                     DVIILDKAAGMVVHPGVGNWTGTLMNALVYHFEHLPHFPDQLHRPGLVHRIDKNTSGL
                     MVIARSERALVTLAKEFFERTIDRKYLALVWG"
     gene            complement(595..2451)
                     /locus_tag="P5292_01925"
     CDS             complement(595..2451)
                     /locus_tag="P5292_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37913.1"
                     /translation="MHRRLRLPLALLCLFLTFSSSFAQEVVFPLFGNSELQQAASSKH
                     VNQSERRSASSLVQLPFSDDFSRQGFYPNEGLWQDSNVYVNPYYGENPPTIGVATFDG
                     LNRWGNPYNINAAASQKSDYLTSQPVELGTLGPDTSAVWLSFYYQPQGLGDRPESGDS
                     LVLQFLDNAGAWNNQWAVAGRSDTVFQRVSVQVRGAQYLYSGFQFRFFNYATVNGNRD
                     HWHLDYVILNKNTQPNDSIRDNAFIRPQTSLLSEFSALPYSHYKEISPGTAAMRASIS
                     DTVHNLNYGPVSFTYQSSISDALGNTLFLSPQSSLSSTSNTYTDFTTSLNGFSFPAQP
                     GDSADFLLKTWFGQPQLSNPYNDTNYYQQRFRNYYAYDDGSAELGYGVTGNADVWLAY
                     QFDVKKADTLRGIQIHFNPTGVNIATQLIQIAYWDQLSVAGNTHRLVYKMINQRPDTN
                     DYTNGFVTYLFDTLLVVQPGPAWIGFIQNNPQTLFGIGLDRNTDPGDKKFYHADGAWF
                     QSAIEGAWLMRPLFGDTITRGQVIGIEELDRGPILRVFPNPSTGSVRMERPAAAIGNW
                     RYELMESTGRVLRTEQSAQTGLFWQDLPAGYFLLRVTDDKGVSSCFKIVVSQ"
     gene            complement(2490..3239)
                     /locus_tag="P5292_01930"
     CDS             complement(2490..3239)
                     /locus_tag="P5292_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRS37914.1"
                     /translation="MAKKSTGKILLYNLIAAVLVSALVLGGTYSWLSSYTHHGESISV
                     PDVKGMSIKKTEKFLNEKNLEYKVVDSMFVLGKKPGEVLEQDPAADSKVKEGRTIYLT
                     VNASRPPKVKMPNLVDVSFRQAEAILQSFGLKVGKTSYQPDLAKNAVLEQHYKGRTIK
                     PGTEIDKGSVIDLVLGDGVGNAEVLVPDLVGLTRGEALFALKGSSLNVGTLHIDPGTR
                     DTNNAKVYRQIPEADGSTTLRLGEAVDLYLR"
     gene            complement(3253..>5787)
                     /locus_tag="P5292_01935"
     CDS             complement(3253..>5787)
                     /locus_tag="P5292_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRS37915.1"
                     /translation="KGSIRDFYPPVDQLPDSPNEFQVYAESLGKPDRELQDRLIREIG
                     KKVLPDQLNELIDFAFLCLHGPFGEDGRIQGLLEFYGIPYSGSGILSSAIGINKAIQK
                     SLMHEAGFPGPANFSIRRSEWADPMTAGGIITKIRDEIGYPCVVKPANQGSSIGVTIL
                     SAEDPARLAEAIELAFFRQRISQEEWKGLDHDHKLAELRSLCDIREGIGMPVRCTAGT
                     ECTILYHPEDLLRFLDNRFASGTESVLLEGLDADTEVLVEQFIDGKEFSCIVIRNANG
                     TPVALPPTEIRKGRELFDYRSKYLPGLSRKITPIELPDDQIRQIRKECERLFLQLHFG
                     VYARIDGFMTQDGRVFLNDPNTTSGMMPSSFFFHQAAEIGLNPSQFLTYIIRTSLWER
                     NHDLKEIVRTPALLKRMDAGLAGLRQLENAKTRVAVIMGGYSSERHISVESGRNVYEK
                     LSSSDHYEPFPVFLTGDEQEHELWQIPVNLMLKDNADDIRHLVQHHKVHPVIREIMQE
                     CEGITSEYTDQRNVLHPRKISYHELAEIADVVFIALHGRPGEDGEIQKRLETVGLPYN
                     GSGTDSSRITINKYETNELLMRNGFSVARHALADREEWKRDPEGFYRSIIQRFPFPFI
                     AKPVDDGCSSAVKKIRNEDDLRAFSEMIFRVEEDLLAGPASVLQLKAKEEFPRKRQFL
                     LEELVTRKDAAHFLEITGGLMTSLDDRGKRVYEVFEASEALSEGDVLSLEEKFLAGEG
                     QNITPARYARDPKERNRISEIVKRELQKAAELLNVEGYARIDAFVRVFPNGKVEVIFI
                     EVNSLPGMTPATCIFHQTALAGYKPYEFIDRILTYGIRRKHASVGI"
BASE COUNT         1260 a         1468 c         1652 g         1407 t
ORIGIN      
        1 accccagacc aatgccaggt atttgcgatc gatcgttcgt tcaaagaatt ctttcgccag
       61 ggtgacgagc gcccgttcgg aacgtgcgat gaccatcaga cccgatgtat tcttgtcgat
      121 gcgatgtacc agtccgggcc gatgcaattg atcggggaaa tgggggagat gctcgaaatg
      181 atagaccagc gcgttcatca acgttccggt ccagttgccg actcccggat ggaccaccat
      241 acccgctgcc ttatcgagga tgatcacgtc ttcgtcttcg aacacgatgt tgagcggaat
      301 attttcggga accagttcaa attccctgac cggctccggt aagactacct ggacctggtc
      361 cagcggtttt accttataac tcggcttggt ttcctttcca ttaaccagga tgcacccggc
      421 atcgcaggct tgctggatct tattccgcga tgcaccggta agccggttca tcaggtattt
      481 gtcaacacgc aagggcgcct gacctttatc gacgacgaat cgatggtgct cgtagagttc
      541 gtcgtgatcg gcggcttcga ggtcgatcgg ttccatatga tgccccaagg ggacttactg
      601 gctaacgacg attttgaaac agcttgacac gcctttatcg tccgttaccc gcaaaagaaa
      661 atagccggcc ggaagatctt gccagaaaag tcccgtctgt gcggattgct cggtgcgaag
      721 tactcgtcca gtactctcca tgagttcgta gcgccaattg ccgatggcgg cggctggtcg
      781 ctccatacgc acggagccag tggacggatt cggaaaaaca cgtaggatcg gtccgcgatc
      841 gagctcctca ataccgatca cttgcccgcg tgtgatggtg tcgccgaata acggtcgcat
      901 caaccaggcg ccttcgatgg cggattgaaa ccaggcgccg tcggcatgat agaatttttt
      961 gtcaccggga tcggtgtttc ggtcaaggcc gatgccgaag agtgtttgcg gattattctg
     1021 gataaacccg atccatgccg gtccgggttg taccaccaac agggtgtcga agaggtaggt
     1081 cacaaatccg ttggtatagt cgttggtgtc gggccgttga ttgatcatct tgtaaacaag
     1141 gcgatgggta ttgcccgcca cactgagctg gtcccaatac gcgatctgga tcaattgcgt
     1201 agcgatgttc accccggtag gattgaagtg gatctggatg ccgcgaaggg tgtccgcttt
     1261 ctttacatcg aactgatagg ccaaccacac atccgcgttt ccggtgaccc cgtatcccaa
     1321 ttcggcggag ccatcgtcgt atgcatagta gttgcgaaag cgttgctggt aatagttggt
     1381 gtcgttatac ggattcgaca attgaggctg accgaaccag gttttgagca ggaagtcggc
     1441 ggaatcgccc ggttgagccg ggaaactgaa accgttcagg gaagtggtga agtccgtgta
     1501 ggtattcgac gtggagctga gtgaactttg cggggagaga aaaagcgtgt taccgagcgc
     1561 gtcgctgatg ctgctttggt aggtgaagga gacggggccg tagttcaggt tgtgaacggt
     1621 atcgctgatg gaggcgcgca tggctgcggt gccaggagaa atttccttgt aatgtgagta
     1681 gggcaacgcg ctgaattcgg agagcagaga agtctgcgga cgaatgaagg cattgtcccg
     1741 gatggaatcg ttcggttgtg tattcttgtt caggatcaca taatccaggt gccagtggtc
     1801 ccggtttccg ttgacggttg cataattgaa aaagcgaaac tggaatccgg aatacaggta
     1861 ttgtgcaccg cgtacctgta cactgacacg ttgaaatacg gtatcgcttc tgccggccac
     1921 ggcccattgg ttgttccagg cgccggcatt gtcgagaaac tgcaacacga gtgaatctcc
     1981 ggattcggga cggtcgccca atccctgcgg ctgataatag aaactcagcc agaccgccga
     2041 tgtgtcggga ccaagtgttc caagttcgac aggttgggag gtgaggtaat cggatttctg
     2101 actcgcagct gcattgatgt tgtaagggtt gccccagcgg ttcagaccgt cgaaggtagc
     2161 gaccccgatc gtaggcgggt tctcgccata gtaaggattc acatatacgt tactatcctg
     2221 ccaaaggcct tcattcgggt aaaagccctg acgggaaaaa tcatcagaaa acggaagttg
     2281 aaccagagaa ctggcgctcc gtcgttccga ttgatttaca tgttttgagg aagcggcctg
     2341 ctgcaattca gaattcccga aaagtggaaa cacgacttcc tgagcgaagg aactggaaaa
     2401 tgtcagaaac aaacaaagca gggccagagg taaccgcagt cgccggtgca tcatgggata
     2461 aaggtaggat ttatggctga atgacgggtt tagcgcaagt ataagtctac tgcttcaccc
     2521 aatcgcaagg tcgtgctgcc gtcggcttcc gggatctggc gatagacttt cgcgttattg
     2581 gtgtcgcggg tacccgggtc gatgtgcagt gtcccgacat tcagggaaga acctttgagc
     2641 gcaaacaagg cttcgccgcg tgtcagtcca accaggtcgg gtaccagcac ttcggcattt
     2701 ccgacgccat cgcccagtac gaggtcaatg accgatcctt tgtcgatctc ggtgccgggt
     2761 ttgatcgtgc gacctttgta atgctgttcg aggacggcgt tttttgcgag atcgggttga
     2821 taggaagtct tgccgacctt cagcccaaac gactggagga tcgcttccgc ctgacggaat
     2881 gaaacgtcga ccaggttggg cattttcact ttgggggggc gactggcatt gacggtcagg
     2941 tagatcgtgc gaccttcctt gaccttcgaa tctgccgcag gatcctgttc caatacttct
     3001 cccggtttct tccccaggac gaacatggaa tcgacgactt tatattcgag gttcttctcg
     3061 ttgaggaact tttctgtttt tttgatcgac attcctttca catccggaac cgaaatactc
     3121 tctccgtgat gcgtatagga gctgagccaa ctgtacgtgc cgccaaggac cagcgcggaa
     3181 acaagtacag cggcgatcag gttgtaaagc agaattttgc cggtagattt ttttgccaca
     3241 gcgaatgagt tgtcagattc cgaccgatgc gtgtttcctc cggatgccgt acgtaaggat
     3301 ccggtcaatg aattcatacg gtttgtatcc ggctaaagcg gtttggtgga agatgcaggt
     3361 tgccggcgtc atgcccggca ggctgttgac ctcgatgaaa atcacctcta ctttcccatt
     3421 cgggaaaacg cgaacgaacg catcgatccg cgcatatcct tccacattga ggagttccgc
     3481 ggctttttgc aattcccgct tcacgatctc tgaaatcctg ttccgttcct tcggatccct
     3541 tgcatagcgc gccggggtga tattctgacc ttcacctgcc aggaacttct cttccaggga
     3601 aagcacgtcg ccttccgaca aggcttcgga tgcttcgaag acttcgtaca cccttttccc
     3661 gcgatcatcg aggctggtca tgaggccacc ggtgatttcc agaaaatgcg cggcgtcttt
     3721 cctggttacc aactcttcga gcaggaattg tcgttttctt ggaaactcct cctttgcttt
     3781 caactgcaag accgatgccg gcccggccag caggtcttct tcgacccgga agatcatttc
     3841 cgagaatgca cgcaaatcat cttcgttccg gatcttcttc accgcgctgc tgcaaccgtc
     3901 atcaacgggt tttgcgataa acggaaaggg gaagcgttgg atgatggagc gataaaaccc
     3961 ttccggatca cgtttccatt cctcccggtc ggccagcgcg tgacgtgcaa ccgaaaatcc
     4021 attgcgcatc agcagttcgt tcgtttcgta tttattgatg gtgatgcgcg aagaatcggt
     4081 ccccgatcca ttgtagggca agcccacagt ttccaggcgt ttctggatct cgccgtcctc
     4141 gcccggacgg ccgtggaggg cgatgaagac gacatcggcg atctcggcca attcgtggta
     4201 ggagattttt ctcggatgaa ggacgtttcg ttggtcggta tactcgctgg tgatcccttc
     4261 gcattcctgc atgatctccc ggatgacggg gtgcaccttg tggtgctgga caaggtgccg
     4321 gatatcgtcg gcgttatcct tgagcataag attcaccgga atctgccaaa gttcatgttc
     4381 ctgttcgtcg ccggtgagga aaaccgggaa gggttcgtaa tggtcagaac tcgacagttt
     4441 ctcgtacacg ttcctgccac tttcaacgga gatatgtcgt tcagaagaat agccgcccat
     4501 gatcacggct acccgggttt tagcattctc caattggcgt aaacccgcca gtccggcgtc
     4561 catgcgttta agcaaggccg gtgtccggac gatttccttc aggtcatgat tgcgttccca
     4621 gagggaagta cggatgatgt atgtcaggaa ctgtgagggg tttagtccga tctcggctgc
     4681 ctggtgaaag aagaacgaag atggcatcat acccgaggtg gtattcgggt cgttcaggaa
     4741 gacgcggccg tcctgcgtca tgaacccgtc gatgcgggcg tagacgccga aatgcagttg
     4801 gagaaagagg cgttcacatt ctttccggat ctggcgtatc tgatcatcgg gaagttcgat
     4861 gggtgtgatc tttctcgata aacccggcag gtacttggag cgatagtcga ataactcgcg
     4921 gcctttgcga atttcggtgg gtggcaaggc taccggtgta ccgtttgcat tccggatgac
     4981 gatgcaggag aactcctttc catcgatgaa ttgctctacc agcacttcgg tgtcggcatc
     5041 caggccttcg aggagtaccg actcggttcc actcgcgaag cggttatcca ggaatcgcag
     5101 tagatcttcc gggtgataga gtatggtgca ttcagtaccc gcggtacaac ggacgggcat
     5161 accgatccct tcgcggatgt cacagagtga acggagttca gcaagcttgt ggtcgtggtc
     5221 cagccctttc cattcttcct gactgatccg ttgtcggaag aaagcgagtt cgatcgcttc
     5281 cgcgagtcgg gccggatctt cggcggaaag gatcgtgaca ccgatggatg aaccctgatt
     5341 agccggcttt acgacgcagg gatatccgat ctcgtcgcgg atcttcgtga taataccacc
     5401 ggcggtcatg gggtccgccc actccgaacg acgaatggaa aaattggcgg gtccggggaa
     5461 gccggcttcg tgcatcaggc ttttctggat cgccttgttg atgccgatgg cggaagagag
     5521 gatgcccgag cctgaataag gaatgccgta gaattccagg agaccctgga tccgaccgtc
     5581 ttctccgaac ggaccgtgca ggcagaggaa ggcgaaatca atcagctcat tcagttggtc
     5641 gggaaggact ttcttgccga tttccctgat aaggcggtcc tgtagttccc ggtccggttt
     5701 gcccaggctt tcggcataga cctggaattc gttcgggcta tcgggtaatt gatcgaccgg
     5761 cggataaaaa tcccgaatgc ttccttt
//
LOCUS       DAOTON010000057         5772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_11801_length_5772_cov_3.624628, whole genome shotgun sequence.
ACCESSION   DAOTON010000057 DAOTON010000000
VERSION     DAOTON010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11801_length_5772_cov_3.624628"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="P5292_01940"
     CDS             <1..1053
                     /locus_tag="P5292_01940"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37916.1"
                     /translation="INYRLCNYGTDALTSATITLRDGATTIDTYNWTGNLATYESIPL
                     AFNGVNGVMGANTISVVVSDPNGQSDGNTTNDERSVQFTIFNSVGGPAVSETFASASF
                     PPSGWSELNGGDVAGWEYSTAGLNGAGSAKMNFVYSPAGDFDAIVMPTMDMTGYDNAV
                     LTFDIAAARYGTSNDNLKVKVSYNCGLTWTTLYNKTGAALATVGSQTSIFTPTNANQW
                     RAESINLNNYLTRPALLVKFEAFSNQGNNLYLDNINMSLTTGLNIVNRALDFSLSPNP
                     ARSLAMVNFSLAKQQAVQLSVLDAQGRIVYAFDETSVLPGDHQLFVPVSGLAEGIYLV
                     RLQGEDGQQLRRLVVE"
     gene            1187..1891
                     /locus_tag="P5292_01945"
     CDS             1187..1891
                     /locus_tag="P5292_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37917.1"
                     /translation="MITRELDLIGELTSPLFRILWTHDADEPARRQFENNIVAFHIGK
                     GYILTVAHNLRSEAPLIKSLPDAVYQAEIRPRLPDLQRQVFEQLYPLDQSNGRRYPRL
                     TAANDARNIGEFLRQNNIDTRWITLMRQQVCRPQLILQFKHPEYFGDAKLAAGFSPTR
                     RFHEPAINRHTFLVDLELVKHFFAKTSRFTGSLICRKRCLTRSRLWNRTSACSMPFRP
                     ICIVFKVPRTVKWADS"
     gene            1876..2202
                     /locus_tag="P5292_01950"
     CDS             1876..2202
                     /locus_tag="P5292_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37918.1"
                     /translation="MGRLLNRAVVEGYLEHFGMFPDRIGGHYTLEGMRYLIKGYFRFG
                     SSGAPYIRRNEQGRFVVNAIQSEASPIQLSIGNAREGNFQYVNAIASPLSGIEEQLRN
                     HLNVSA"
     gene            2367..5276
                     /locus_tag="P5292_01955"
     CDS             2367..5276
                     /locus_tag="P5292_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37919.1"
                     /translation="MILWLLILVAGDRSVCTAANVGVQAAFVKNNTIQDASEVAANVL
                     RIVNYTNKKVRFGVSVGLPAGWITLGNRDRMIDLPAGDSVFIPVRAIPDRQAKGGTSY
                     VLSAILRSEQDQQFAADNWYVTIKSHSDWRVNVPQRQIYFTNGSDSANFSVQISNQGN
                     SDESIRLVFLPDRRIQLLDPVTKQPAALTITCDLPVGHDTVVRYAVRKVTEQRRSAGK
                     KDLTAAADPNRESYPVQVLARSAGGTGNRSWSGNITFARVGNRVAQHLYSGNALPLTM
                     EVNLFDVLTDGTTMSLDLYGSTNLRNNAFLTYRLQSVFITNYLNEQAFLGQNHYIGYF
                     SNRYTVEAGEVNGWGRSLLTGRGLKASVKVRNETIGAIYVRGPGLFRNNPNSGYGLYH
                     QHAKGPLTWSNYFTRNENRQLKLTTNAANTYASYKAAPGHQLGFGMGMSSERFRPDTA
                     EGSFGAGYGFDANYSGTYGPYGFFANYNYGSSQYALYRGVRMFNTRLSWAISQDRNLS
                     LNTQHFTQRPEYFVNGRIIDGPFLRNQRYELRYGIFMPKAMIAFRPLYQFDENPRIRV
                     RTTGMGFDYNVRNNEAMRVSTNLFMGYGFAPTVGLDPFFLARAGVYARWNTTFLSLRY
                     FYGPNQLQEQIRFISYRINPQSVFLTGSNDYWFGNGKFVLTTTGNLFYETFYKRLNTR
                     IRPELFYFTNTGFRFSMYVSWVRNAQGSNPVLNEITGEETFEPVANADFNIGFGVKKQ
                     LGVPLPGPKYISTKVIVFRDMNGNRRQDGQEDGVENILVTIRPKRVEGGDTLSYSIVH
                     GEDFITDKKGEVWYDNITPGIYYMRMSALVGQGDWFTGEEQEVVVRKGEPVYLPLNRG
                     LRLNGSILVDRDKYSGGGEGTMDLSRIRITAIDSLGHTYTCLSERDGTFNLQLPMGKY
                     TLTANEAALGDGFLFLRNKIELDLTRYVENYSVTFNVVERKRKVEIKRFGGSEKR"
     gene            5347..>5772
                     /locus_tag="P5292_01960"
     CDS             5347..>5772
                     /locus_tag="P5292_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37920.1"
                     /translation="MKLRSLILMLLGSCLMPLLAGAQSVSMSPSRLYYKVDVGAYKTQ
                     TVTVTNNSSVRQAFNVSFGDFEPAGYQGKSKFMQAGESEHSCSEWLTATPSFFELDPG
                     QSQKVQVLLQVPTSPEANKVKWAAMRVKLTKERKSNDIAD"
BASE COUNT         1332 a         1612 c         1542 g         1286 t
ORIGIN      
        1 atcaattacc gcttgtgcaa ctacggaacg gatgcactta cctccgctac cattaccctg
       61 cgtgacggtg caaccacgat cgatacgtac aactggacgg gtaacctcgc gacctacgaa
      121 tccatccccc tggcattcaa cggtgttaac ggcgtcatgg gcgccaacac catttcggtc
      181 gtcgtgtcgg atccgaacgg acaatccgat ggcaacacga ccaatgatga gcgcagcgtg
      241 caattcacga tcttcaactc cgttggcgga cctgctgtca gtgagacatt tgccagtgca
      301 agcttcccgc cctccggctg gtcggagttg aatggcggtg acgtggctgg atgggaatac
      361 agtactgccg gtctgaatgg cgccggatcc gccaagatga atttcgtgta ctccccggca
      421 ggcgatttcg acgcgatcgt gatgccgacc atggacatga cgggctacga caatgccgtt
      481 ctcaccttcg atatcgcggc tgcccgttat ggcacttcga acgacaacct caaagtgaaa
      541 gtctcctaca actgcggcct gacgtggacc accttgtaca acaagaccgg ggcagcgctt
      601 gccaccgtgg gttcgcaaac ctccattttc acaccgacca atgcgaacca atggcgcgcc
      661 gagtccatta acctgaacaa ctacctgacg cgaccggctt tactggtcaa gtttgaagcg
      721 ttcagtaatc agggaaacaa cctgtatctc gacaacatca acatgagcct gaccaccggc
      781 ctgaatatag ttaaccgcgc tctcgatttc tccctgtcgc cgaacccggc ccgctcgctt
      841 gctatggtca acttcagtct ggctaagcag caggccgtac aactcagtgt gctggatgcg
      901 cagggccgga tcgtctacgc gtttgatgaa acctccgtgc ttcctggcga ccatcagctc
      961 tttgttccgg tctccggact ggcagaaggt atctacctcg tccgcctgca aggcgaagac
     1021 ggacagcagc ttcgccgctt ggtcgtagaa taatcatcct gtggtggacg gaacgctccc
     1081 ggtcttgtgg tccgggagcg ttcttttttg taagaaatcc tgctgcattt ggttgttaat
     1141 tctgctgcgt atgtaaccct gtcggtgtcg tacgtaataa gaaggaatga ttacccgtga
     1201 acttgatctc atcggcgaat tgacctcgcc gcttttccga attctgtgga cgcacgatgc
     1261 cgatgaaccc gcgcgtcgcc agttcgagaa caacatcgtg gcgtttcata tcgggaaggg
     1321 ttatatcctg accgtcgccc acaacctgcg atcggaagcg cctttgatca agtcgctgcc
     1381 ggatgccgtt taccaggcgg agatacgtcc acgtctgccg gacttgcaac ggcaggtgtt
     1441 tgaacaactg tatccgctcg atcaaagcaa cggccggcgt tatccgcgcc tgaccgcggc
     1501 gaacgatgcg cgaaacatcg gggagttcct gagacagaat aatatcgata cccgctggat
     1561 cacgctgatg cgccagcaag tttgccggcc acagctcatt ctgcagttta agcatccgga
     1621 atacttcggc gatgcgaaac ttgcagccgg cttcagcccc acgcgcagat ttcacgagcc
     1681 ggcgatcaac cgacatacgt ttctggtcga cctcgaacta gtcaagcatt ttttcgcgaa
     1741 gacatcgcgc tttaccggat ccttgatttg ccgcaagcga tgcttgacaa gatcccgttt
     1801 gtggaaccgg acttcggctt gctcgatgcc tttccgtccg atttgtattg tcttcaaagt
     1861 tccccgaaca gtgaagtggg ccgactcctg aaccgtgccg tcgtagaagg ctatctcgaa
     1921 cacttcggca tgttccccga ccgtatcggg gggcattata cgttagaggg catgcgttac
     1981 ctgatcaaag gatatttccg tttcggttca tccggtgcgc cgtacatccg gaggaatgaa
     2041 cagggccgct tcgttgtcaa cgcgatccaa agcgaagcca gtcccatcca gttgtcgatc
     2101 ggcaacgcac gagaagggaa tttccaatat gtgaacgcga tcgcgtcgcc tttgtccggc
     2161 atcgaagagc aattgaggaa tcatctcaac gtttcggcct gatttcgttt tgcgaatccc
     2221 gcattctttt tcatttgaga aagtgcgcaa gacaattttg taataaacct ttcattttta
     2281 cgctaaccga cagccggtgc tcccgttacc atctataagg aataatggac ccggttacgc
     2341 caattttaag gcaaatttca aggatattga tcctttggct actcatcctg gtagcagggg
     2401 atcgatccgt ctgtaccgca gcgaatgtcg gtgtccaggc cgcctttgta aagaacaata
     2461 cgatccagga tgccagcgag gtagcagcga atgtcctgcg gattgtcaac tacaccaaca
     2521 agaaggtccg attcggtgtc tcggttggat taccggcggg ttggatcacg ctgggtaatc
     2581 gcgatcgcat gatcgactta ccggccggtg actccgtctt tattccggtt cgtgcgatac
     2641 ccgaccgaca ggcgaagggc ggcaccagct atgtgttgtc cgccatcttg cgttccgagc
     2701 aggatcaaca gttcgcggcc gacaactggt acgtaaccat caagtcgcat tccgactggc
     2761 gggtaaatgt tccgcagcga cagatctatt tcaccaatgg ttccgactcg gccaacttca
     2821 gcgttcagat cagcaaccag ggaaactcgg acgagagcat ccgattggta ttcctgccgg
     2881 atcgccggat tcagttactc gatcctgtaa cgaaacaacc cgccgcgctg acgatcacct
     2941 gcgatcttcc cgtagggcac gataccgttg tgcggtacgc ggtacgaaag gtgacggagc
     3001 agcgccgctc ggcggggaaa aaagacctga ccgctgccgc ggatccgaac cgggagagct
     3061 atcccgtaca agtgctcgcc cgatcagccg gaggaaccgg caaccgaagc tggagcggca
     3121 acatcacctt cgcccgggtc ggtaaccggg tcgcgcagca tctttattcg ggaaacgcgt
     3181 tgccgttgac catggaagtc aacctgttcg acgtattgac ggacggaacc acgatgtcgc
     3241 tcgatctata cggcagtacg aacctgcgca acaacgcctt cctgacgtat cgcctccaaa
     3301 gcgtctttat cacgaactac ctcaacgagc aggcgttcct cggacagaac cattacatcg
     3361 gttacttctc caaccggtac acggtggaag caggagaagt aaacggatgg ggaaggagtt
     3421 tgctgacggg ccggggattg aaggcatcgg tgaaggtgcg gaatgaaacc attggggcta
     3481 tctatgtccg cggaccgggt ttattccgca acaacccgaa cagcggatat ggtctctatc
     3541 atcagcatgc taaaggcccg ttgacctgga gcaattattt tacgcggaac gagaaccggc
     3601 agttgaaact caccaccaat gccgccaaca cctacgcgag ttacaaggcc gcacctggtc
     3661 accagctggg ttttggtatg ggcatgtcgt ccgagcggtt ccgtccggat actgcggagg
     3721 gaagcttcgg tgccggctac ggtttcgacg cgaactactc gggtacatac ggtccgtatg
     3781 gcttcttcgc caactataat tacggttctt cccaatacgc cttgtatcgc ggcgtccgca
     3841 tgttcaatac gcggctttcc tgggcgatct cacaagaccg gaatctttcc ctgaacaccc
     3901 agcatttcac ccagcgtccc gagtatttcg tcaacggccg gatcatcgat gggccgttcc
     3961 tgcggaacca gcggtatgaa ttgcgttacg gcatcttcat gccgaaggcg atgatcgcct
     4021 tccggccact ctaccagttc gacgagaacc cgcgcattcg tgtgcggaca accggtatgg
     4081 gcttcgacta caatgtgcgg aacaacgaag ccatgcgtgt ttcgaccaac ctcttcatgg
     4141 ggtatgggtt tgcacctacg gtcggacttg atcccttctt cctggcgcgt gccggcgtgt
     4201 atgcacgttg gaacaccacc ttcctcagcc tgcgttattt ctatggtccc aaccagttgc
     4261 aggaacagat ccggttcatc agctatcgga tcaatccgca atcggtattc ctgaccggat
     4321 cgaacgacta ttggttcggc aacggaaagt tcgtgctgac gaccacgggt aatctctttt
     4381 acgagacatt ctataagcga ttgaacacgc gcattcgtcc tgagttgttc tatttcacga
     4441 acaccggatt tcgtttttcc atgtatgtct cctgggtgcg aaacgcccaa ggttccaacc
     4501 cggtcttgaa cgagatcacc ggagaggaaa ccttcgaacc cgttgccaat gcggacttca
     4561 acatcggctt cggcgtgaag aagcagcttg gcgttccctt gccgggtccg aaatatattt
     4621 ccacgaaagt gatcgtattc cgcgacatga acggcaaccg ccgccaggac ggacaggaag
     4681 atggtgtgga aaacatcctg gtgaccatcc ggcccaagcg cgtggagggt ggtgatacct
     4741 taagttacag catcgtacac ggggaggatt tcatcacgga taagaaaggc gaagtgtggt
     4801 acgataacat cacacccgga atctattaca tgcggatgag cgcactcgtc ggacaaggcg
     4861 actggttcac cggcgaggaa caagaggttg tggtgcggaa aggcgaaccg gtttatctgc
     4921 cgctgaaccg gggcttgcgt ctgaacggta gcatcctcgt ggatcgcgat aagtactcgg
     4981 gtggaggaga aggtacaatg gacctgtccc gcattcgcat caccgcgatc gattcgctgg
     5041 gtcatacgta cacctgcctt tcggaacgcg acggaacatt caatttgcaa ttgccgatgg
     5101 gaaaatatac gctgacggcg aacgaagccg cgctcggaga cgggttcctg ttcctccgca
     5161 acaagatcga gctggacctg acccgctatg tggagaacta cagcgtgacg ttcaatgtcg
     5221 tagaacgtaa gagaaaggtc gaaattaagc ggtttggtgg cagtgaaaag cgataaatgc
     5281 agcaacccga cccgtaaccc ggcgtaacac cttcagaaaa tccaaccaac caaacgaata
     5341 ttcatcatga agctccgcag tcttattctc atgctcctgg gttcctgcct gatgccttta
     5401 ttggcaggcg cgcaaagtgt aagcatgtcg ccgagccgcc tctattataa ggtggatgtc
     5461 ggagcgtaca agacgcaaac cgttaccgtc acgaacaatt cttcagtccg acaggccttc
     5521 aacgtttcgt tcggtgactt cgagccggcc ggttatcagg gcaagagcaa gtttatgcaa
     5581 gccggtgaga gcgaacattc ctgttcggaa tggttgaccg ctacgccttc gttcttcgaa
     5641 ctcgatccgg gtcaatcgca aaaggtacag gtgctgctgc aagtgccgac ctcgcccgaa
     5701 gcgaacaaag tgaaatgggc cgccatgcgc gtcaaactca ccaaggaacg taagtcgaac
     5761 gacatcgccg ac
//
LOCUS       DAOTON010000058         5764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_11820_length_5764_cov_3.009634, whole genome shotgun sequence.
ACCESSION   DAOTON010000058 DAOTON010000000
VERSION     DAOTON010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11820_length_5764_cov_3.009634"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..647
                     /locus_tag="P5292_01965"
     CDS             210..647
                     /locus_tag="P5292_01965"
                     /inference="COORDINATES: protein motif:HMM:NF040124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HRS37921.1"
                     /translation="MKRQSYSNHRRFVWWFHYLLLALCVLFFLGSSAYLYRVVRHAAG
                     DLLLASLMSLLSVVLLLFYYTLRKFPLGVQDRIIRAEENFRHYQLTGQHLDHRLHLRQ
                     IIALRFAPDDEFVNLCKEAIERELSETEIKKKIRRWRGDYHRI"
     gene            803..1759
                     /gene="paaA"
                     /locus_tag="P5292_01970"
                     /pseudo
     CDS             803..1759
                     /gene="paaA"
                     /locus_tag="P5292_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
     gene            1791..2075
                     /gene="paaB"
                     /locus_tag="P5292_01975"
     CDS             1791..2075
                     /gene="paaB"
                     /locus_tag="P5292_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015034815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HRS37922.1"
                     /translation="MEKDHWPVWEVFTQKKSGLPFEHAGSLHAPDKEMALMNARDVYS
                     RRNEATCIWVVPAESITSSTPTDMGPFFDPSNDKAYRHPNFYKTPGASFD"
     gene            2116..2874
                     /gene="paaC"
                     /locus_tag="P5292_01980"
     CDS             2116..2874
                     /gene="paaC"
                     /locus_tag="P5292_01980"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HRS37923.1"
                     /translation="MTKQEALFEYCLRIGDTGVVLGQRLGEWCGHGPVLEEDIAMTNI
                     ALDLIGQARGFLTYAGEVEGKGRSEDDLAYMRSDREYRNVLLAEQPNGDFAVTMMRQF
                     LISAFYHHFFQALTKSKDSTLAALGEKSLKEVTYHLRHSRDWMLRLGQGTEESRRRLL
                     AALDELWTYTGDLFDMDTVDELLVREGIGVDLKSIRPLWEHTVRETFTEAEIPYPADS
                     FMQRGSRDGRHTEHLGHLLSELQILHRSIPNAQW"
     gene            2922..3407
                     /gene="paaJ"
                     /locus_tag="P5292_01985"
     CDS             2922..3407
                     /gene="paaJ"
                     /locus_tag="P5292_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HRS37924.1"
                     /translation="MVTTDQVWRILEEIPDPEIPVISIVDLGIAREVNIHDTGVEICI
                     TPTYSGCPAMHAIENDIVRKLTEEGIPNVRVRMVYSPVWTTDWITDAAKERLRQYGIA
                     PPEKSTSDKGALLGKTREVACPRCGAKHTSLVSEFGSTACKALYRCEVCKEPFDHFKC
                     H"
     gene            3444..4232
                     /locus_tag="P5292_01990"
     CDS             3444..4232
                     /locus_tag="P5292_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRS37925.1"
                     /translation="MTEQHPLVITELRGSVLCITFNRPDKFNSFNRQLALELQAALDR
                     AANEANIRAVYMTGNGKAFCAGQDLSEAIAPDGPGIQRIVREHYNPVIRMIRSLEKPV
                     ICAVNGVAAGAGANIALACDIVIAGKSASFIQAFSKIGLVPDSGGTFTLPRLIGWQRA
                     SALMMTGDKVGADDALAMGMVYKVCEDEALTTTAFGIAETLSRMPTKGIGLTKRLLNQ
                     SFDNNLEQQLAAEETEQAAAGTTYDYQEGVQAFLEKRKPEFRGS"
     gene            complement(4297..4569)
                     /locus_tag="P5292_01995"
     CDS             complement(4297..4569)
                     /locus_tag="P5292_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRS37926.1"
                     /translation="MNKAELIEAISGEAKITKADASRALEAFMTVTTKTLKKGDRVAL
                     VGFGTFSVSKRAARNGRNPQTGKPIKIAAKKVAKFKAGAELSSKLK"
     gene            complement(4741..5508)
                     /locus_tag="P5292_02000"
     CDS             complement(4741..5508)
                     /locus_tag="P5292_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRS37927.1"
                     /translation="MAKQHPGKNGNHRRDAERYFVQGPHSRWRELGFTLGVVREFIRG
                     FRALHFLPPCVTVFGSARFQEDHPHYLLAREMGHALSRMGFTVMTGGGPGIMEAANRG
                     AKDAGGFSVGCNIHLPKEQKPNAYLDRTVTLHHFFVRKVLLLKYSYAFVVMPGGFGTM
                     DEFFETLTLIQTGKISDFPVVLMGKDYWKPLLDLLHQMVREGTAAQKDLEHLIVTDEP
                     GQALAHIRKHAIDEFGLSRFRRPRAFRFFGERRFRIR"
     gene            5597..>5764
                     /locus_tag="P5292_02005"
     CDS             5597..>5764
                     /locus_tag="P5292_02005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599034.1"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the tau chain serves as a
                     scaffold to help in the dimerizaton of the alpha,epsilon
                     and theta core complex; the gamma chain seems to interact
                     with the delta and delta' subunits to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRS37928.1"
                     /translation="MERFIVSARKYRPVTFDTVVGQPHITQTLKNAIRNNHLAQAFLF
                     TGPRGVGKTTTA"
BASE COUNT         1350 a         1575 c         1608 g         1231 t
ORIGIN      
        1 gataccctgc ttcatgcagt gaaagcgccg cgcgatggac ccgttggtcg gtgaccagat
       61 cgctgatgac agcactgatg atccggggtt tttccgtcat ggtgccgcaa agaacggcaa
      121 gtggtccgat attattggcc aatgacagca ggatttcttg gccggcaccg ggtattccgg
      181 tagttttaat tcgttcaact ttgtgtgtta tgaagcgtca aagctattcc aatcatcggc
      241 ggttcgtctg gtggttccac tacctgctct tagccctctg cgttttgttc tttctcggat
      301 cctcggcgta tctgtatcgg gtcgttcgtc atgctgcagg cgacctgctg cttgcatcgc
      361 tgatgagtct gttgagtgtc gtgctgctct tgttctatta caccttgcgg aagtttccgc
      421 tgggtgtaca ggaccgtatc attcgagccg aagaaaactt ccggcactat cagttgaccg
      481 gacagcacct ggatcatcgc ctgcatcttc gtcagatcat cgcgcttcgg ttcgcaccgg
      541 atgatgagtt cgtcaacctg tgcaaggagg cgatcgaacg ggaattgagc gaaacggaaa
      601 tcaagaaaaa gatacgccgc tggcggggtg attatcaccg gatttgagtt gattaactca
      661 accagtctaa ttttaggtcc acaacccgct ttttaggcaa ttttcaaagc ttaccttgga
      721 tatttccgtg aaactgcatg actctggtca ttgttatagc tgtgtgtatt gagtaaattc
      781 ggaacccaat aaatcagcga attccatgat gcaaagcatg actgaacagg aacgggaaca
      841 ggcattccaa aatcgtatcg accagggaga aatcatcgaa ccgaaggatt ggatgcccga
      901 gcgattcaag aagcagttga tccggatgat gagccagcac gcacactcgg aagtggtcgg
      961 tatgttaccg gaaggcaact gggtcacacg tgctcccagt ctgcggcgca aggctgtact
     1021 cctcgccaag atccaggacg aaggtggcca tggcttatac atttattgcg gaacggaaac
     1081 actcggtgtt tcgcgtgagc agttggtcga tgaactgctg agtggaaaag cgaaatattc
     1141 cagcatcttc aactacccgg ctcttacctg ggcggatatg ggatcgatcg gctggctggt
     1201 cgacggcgcg gccatcgtga accaaaccgc cctggccaag tgttcttacg gcccctatgc
     1261 gcgcgcgatg gtgcgtatct gcaaagaaga gaacttccac cacaagcaag gctatgagat
     1321 cctggctacg ctgatgaaag gaacaccgga gcaacgtgcc atggcccagg aatccgtcaa
     1381 tcgttggtgg tggccttcgc tgatgatgtt cggcccgagc gacaaggatt cgcccaacag
     1441 cggtgagctg atgcgttggc gcgtgaagcg ttattcgaat gacgatctgc gacagcgatt
     1501 catcgaccgg actgttccgc aagcggagaa catcggtctg accctgcctg atcccgatct
     1561 gaaatggaac gaagcccgtc agcactatga cttcggcacc atcaactggg aagaattcta
     1621 ccaggtcatc agcggacacg gtgtctgcaa ccgcgagcgt ctgaaagcgc gggtgaaagc
     1681 ccacgaagaa ggccgctggg tccgggaagc cgcgaacgcg tatgcccgca agaaatccga
     1741 aaaagtactg gccgcctgag ccagatcgcc taccaaccac acaacctgaa atggaaaaag
     1801 atcattggcc ggtatgggaa gtcttcaccc agaaaaagtc cggtttgcct tttgagcacg
     1861 caggcagcct gcacgctccg gataaagaga tggcgctgat gaacgcccgc gatgtgtact
     1921 cccgccgaaa cgaagcgacc tgtatctggg tggtaccggc ggaatcgatc acgtcgagta
     1981 ctccgactga tatgggcccc ttcttcgatc cctcgaacga caaggcctat cgtcacccga
     2041 atttttacaa aacacccggc gccagtttcg actgatcgct gaaaaaccgg tcgccagcta
     2101 acggcggcac tagtcatgac caaacaagaa gcgctcttcg aatattgtct tcgcatcggt
     2161 gataccggcg tggtgctggg tcagcgtctt ggcgaatggt gcggacacgg gcccgtgctc
     2221 gaagaagata tcgcgatgac caacatcgcg ctcgacctca tcggccaggc acgcggcttt
     2281 ctcacctacg ccggcgaagt ggaaggcaaa ggacggtctg aagacgatct cgcctacatg
     2341 cgctccgacc gcgaataccg caacgtgctg ctggccgagc aaccgaacgg cgattttgcc
     2401 gtcacgatga tgcgtcagtt cctgatcagc gcgttttatc atcacttctt ccaggctctt
     2461 accaaaagca aagacagcac gctcgccgcg ctcggcgaga agagcctgaa agaagtcacg
     2521 tatcacctgc gtcacagccg tgactggatg ctccgactcg gccagggtac cgaagaaagt
     2581 cggcggcgct tgcttgccgc gttggatgaa ctctggacct atacgggcga tctgttcgat
     2641 atggataccg tggatgaact cctggtgcgc gaaggcatcg gggtcgacct gaagtccatt
     2701 cgtccgctct gggaacatac cgttcgggaa acctttaccg aagcggagat tccttacccg
     2761 gctgattcgt tcatgcagcg cggttcgcgc gatggccgac ataccgagca cctggggcat
     2821 ctcttgtccg aactccagat cctgcatcgt tcgattccga acgcgcagtg gtgagtagcc
     2881 ggcgagtcct tgtttcctac tgagtcaatt tgttttcaac gatggtcacc accgatcagg
     2941 tttggcgaat cctggaagag attccggacc ccgaaatccc ggtcatcagc attgtggacc
     3001 tcggtatcgc acgggaagtc aacatccacg ataccggtgt ggaaatctgc atcaccccta
     3061 cctatagcgg ttgtccggcc atgcacgcga ttgagaacga tatcgtgcgc aaactgacgg
     3121 aagaaggtat cccgaacgtc cgtgtacgca tggtctattc tcccgtctgg accaccgact
     3181 ggatcaccga tgctgccaaa gagcgcctcc ggcaatacgg tatagcgcct ccggagaaaa
     3241 gcactagcga caaaggcgcc ttgctgggca aaacgcgaga agtcgcttgt ccgcgctgtg
     3301 gcgctaaaca caccagcctg gtgagcgaat tcggctcgac agcctgtaaa gcgctttacc
     3361 gctgcgaagt ctgcaaagaa ccgtttgatc atttcaagtg tcattgatcg aatccggtca
     3421 atgattccta ccttctcctc cccatgactg aacaacaccc gcttgttatc accgaactcc
     3481 gtggttcggt cttgtgcatc acctttaatc gacccgacaa attcaacagc ttcaaccggc
     3541 agttggcgct ggagcttcag gcggcgctgg accgtgcggc gaacgaagcg aatatccgcg
     3601 ccgtttacat gacggggaac ggcaaagcgt tttgtgccgg acaggaccta tcggaagcga
     3661 tcgcgcctga cggaccgggg atccagcgca ttgtccgcga gcactacaat ccggtgatcc
     3721 ggatgatccg gagcctggag aagccggtga tctgtgccgt gaacggcgta gcggcaggcg
     3781 cgggtgccaa catagcgttg gcatgcgata tcgtgatcgc aggaaagagc gcgtcgttca
     3841 ttcaggcgtt cagcaagatc ggactggtac cggacagcgg cggcacgttc accttaccac
     3901 gactgatcgg ctggcaacgt gcctccgcac tgatgatgac gggcgacaaa gtcggagcag
     3961 acgacgccct ggcgatgggc atggtgtaca aagtatgcga agacgaagcg ttgacaacaa
     4021 ccgctttcgg aatcgccgag accttgtcgc gcatgccgac gaaaggaatc ggactgacga
     4081 agcggctgtt gaaccagtcg ttcgacaaca acctggagca gcagttggcc gcggaagaaa
     4141 cggaacaagc ggcagccgga accacctacg attaccagga aggtgtacag gcattcctgg
     4201 aaaaacgaaa gccggagttc cggggctcat gacccggatc aaggcataaa aaaaacccgc
     4261 tcgatgagcg ggtttttcat ttcgtagaac tgaaaatcac ttcagcttcg aggacagttc
     4321 tgcaccggcc ttgaacttag cgactttctt agcagcgatc ttgatcggtt tgccggtctg
     4381 cgggttacgg ccgttacggg cagcacgctt agacacggag aaggtaccga aacccacgag
     4441 ggcaacgcgg tcacctttct tcagggtctt ggtggtcaca gtcatgaaag cttcgagcgc
     4501 acgggatgcg tcggccttcg taatcttggc ttcgccagag atggcttcga tcagttcagc
     4561 tttgttcatt gttgttggtt ttagtgatga cgtaaaagta acttcaactc aagcgcttac
     4621 gacagttata aacacaaatg ttgataaata cattcatttg ttgaaaactt aacacgcgaa
     4681 tccgccacca gtgcggcttg tcgaagcagg agcgtttgca ggtcgcgcca gcagtacgat
     4741 tcagcgaata cgaaaacgac gttcaccgaa aaaccggaat gcgcgtggtc gtcggaaacg
     4801 actcagaccg aattcatcga tggcgtgttt gcggatgtgt gccagcgctt gtccgggttc
     4861 atcggtaacg atgaggtgct cgagatcttt ttgcgcagcg gtgccttcgc gtaccatctg
     4921 gtgcaacagg tcgagcagcg gtttccaata gtccttgccc atcagcacca ccgggaagtc
     4981 ggaaatcttt ccggtttgta tgagtgtcag cgtctcgaag aattcgtcca tcgtaccgaa
     5041 accgccaggc attacgacga acgcgtagga gtatttcagc aacagcacct tgcgaacgaa
     5101 gaaatgatgc agcgtgacgg tacggtcgag gtaggcgttg ggcttttgtt ctttcggcaa
     5161 atggatattg cagcctacgc tgaagccacc ggcatccttg gcaccgcgat tggccgcttc
     5221 catgataccg ggtcctccgc cggtcatgac cgtgaatccc atccggctaa gcgcatgtcc
     5281 catctcccgg gccagcaggt agtgcggatg gtcttcctga aagcgggctg agccgaagac
     5341 cgtcacacaa ggcggcagga aatgcaacgc gcggaagccg cggatgaact cccgtacaac
     5401 gcccagggtg aaacccaatt cccgccagcg ggagtgtggt ccctgcacga agtaacgctc
     5461 cgcgtcgcgt cggtgattgc cgttctttcc cgggtgttgt ttggccataa acgcctgtta
     5521 gaatcatcaa atatcgactt agttcccgat ccctgaaact ccgggaatgg ccggacaaat
     5581 cgtatctttg gaacctatgg aacgcttcat cgtctccgcg cggaaatacc gtcctgtcac
     5641 gttcgacacc gtggtagggc aaccgcacat tacccagacg ctgaagaacg cgatccgaaa
     5701 caatcacctg gcgcaggcct tcctcttcac cggtccgcgc ggcgtgggaa aaaccaccac
     5761 cgca
//
LOCUS       DAOTON010000059         5722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_11908_length_5722_cov_3.586907, whole genome shotgun sequence.
ACCESSION   DAOTON010000059 DAOTON010000000
VERSION     DAOTON010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11908_length_5722_cov_3.586907"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1316
                     /locus_tag="P5292_02010"
     CDS             105..1316
                     /locus_tag="P5292_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRS37929.1"
                     /translation="MKTLLSSLQRFSYALALLSFLLFGCGSDTEQQAGRPTSQDSVTM
                     VVAEGKKPMRLVTDRPPNLETPLRYFREDFTPNDVFFVRWHLSNMPTAVNPDTFRLRI
                     SGNVKAPLELSLDELKNDFEQVSVNALCVCAGNARSCLQPRVAGAQWVNGGMGNARWT
                     GVRLKDVLAKAKADARSVAVSFNGMDDPPLETVPDFVKSLPFARANDGEVIIAYEMNG
                     EPLPLLNGYPLKLVVPGWYATYWVGMLNDIRVYADTFHGYWMDKAYRVPKGVSNANES
                     PDSLAKDLEPISRIAIRSIFVSPEPDSVLTKGTRYEMEGLAFDGGSGIARVEISTDDG
                     KSWTETRLDPSLGNYSWRRWRFPYTPAAAGKLRMLVRATGVSGETQPLKHWNRSGYAR
                     NEIESLELTVR"
     gene            1321..1662
                     /locus_tag="P5292_02015"
     CDS             1321..1662
                     /locus_tag="P5292_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRS37930.1"
                     /translation="MTGTSLLRYPFALLLVFYACSRPAEPAARVRTTDPIPLSAVLTE
                     LPEHPDKMLVMANCLPCHSLAYIANQPPMKREAWKKTVRKMIDVFGAPVPDTAVAERI
                     TDYLASIRGSE"
     gene            complement(1796..2809)
                     /locus_tag="P5292_02020"
     CDS             complement(1796..2809)
                     /locus_tag="P5292_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS37931.1"
                     /translation="MKHGNGRDWWIIYRSAPPSNNNFYVYSIDANGVQLDHVDSLGPL
                     HSSNGGDLRFNSDGTKFAFSNLNGLLAIYSFDRCTGSITLDQTIHAEFPAGTQPYFLD
                     CCFSPNDSLLYVSCAPYFSSLDTTQIYLYQFPLTATNVYASRTLIWQQTYPMTIGAVR
                     LAPDNKIYVAAQKTGYPYDSTDYYPENMSLSVINQPDQRGGAAACDFQPYSFYLGGKR
                     TYWGLPNNPNYDLGPLAGSPCDTIQTALQETAAPTPALSVYYHSAWESIVVNASKLEG
                     KRARIFVSDISGKILVEESVILLSGYLSKNIALHNRAAGIYFVTVVTEREKLTGKVIL
                     QSY"
     gene            complement(3310..5577)
                     /locus_tag="P5292_02025"
     CDS             complement(3310..5577)
                     /locus_tag="P5292_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HRS37932.1"
                     /translation="MGDNSRILISPGRTLTIQNSRLSACNALWGGISFADSTGLLILS
                     GDTIEDAQMAIADSAGSRISVTNSVFNRNRQSIVLYGSGAALSSVVSGCTFTCEDGNG
                     LPAQTKDGGYAYSGIETSGLNGWTINANQFRHLYYGVRSENSSITLQACAFRHMRRHA
                     YAYGNARAASYTWENDGFGIYSLNQSGSPVSLTIQGSSFTDCGYGVQARNSIQTTVSG
                     SSFDSLRYAAVSVVNPVAVDVNIHDNSITHYLNGITVSEPRLASSTTIRDNTFSSPSN
                     LNTSPLYNAAVNWSIKLQNRLPGVTTASVSNNHMMDQLKGIYLNGCEEVDINTNFIQL
                     KGAGSTTRQYGIWLVNSSNCTITDDSVVTTSPVTDPTLARGIRFESSTGCSIRNNYLL
                     NLGSGINGLNFCDLTSLCGNDLWNCARGLDVFNITIPQQGDVNAPQDNMWRNIPLANR
                     VVGSTANGLFLKFYHRYTQNDPNNKFSPGNNFIVQSIQSITGLSPCSVPDTLPQLFSE
                     IAEQIINDSIVFLVFNSENKWFADKYAFHLLSKDSMLMNQGLPTDQQRQQFFDDLKLR
                     NVGEIEAILNALNTENYLQAITKLISLQPDNTIEQNFKDVLYLVEKIQTELPLSSSDT
                     VLLDNISSQLAFEGGPAVFIARAIIDVEYDDELTGPLLRQLNNLTDVPDPELVLYPNP
                     SSGKIIISKDLADFGSCRIEVNDLQDRLLLSTSLNQNEAIWEEDLSGLKSGTYLLKIY
                     CDNELVSYNKLILQK"
BASE COUNT         1371 a         1470 c         1516 g         1365 t
ORIGIN      
        1 ggtcgtgatc cccaggacga actgagcggg aaattcaacc accggttgcg gctgaatgat
       61 taacttgtcc tcgccggatg cgccgcatcg cacgatcgtc gacaatgaaa acccttctga
      121 gttctctgca gcgtttcagc tacgcgcttg cgctcctgtc gttcctgttg ttcggttgcg
      181 gaagtgacac ggagcagcaa gccggccgac cgacatcgca ggattccgtc acgatggtcg
      241 ttgcggaggg caagaagccg atgcgcctgg tcaccgaccg tccgcccaac ctcgaaacgc
      301 cgctccgtta cttccgcgaa gacttcacgc cgaacgatgt cttcttcgtc cgctggcatc
      361 tttccaatat gcccacagcg gtcaacccgg atacgtttcg cctgcgaatc agcgggaacg
      421 tgaaagctcc actggaactc tcgctcgacg agctgaagaa tgacttcgaa caagtttccg
      481 tcaacgcgct gtgtgtctgc gccggcaacg cccgcagttg cctgcaaccg cgtgtagccg
      541 gcgcgcaatg ggtcaacggc gggatgggca acgcccgctg gaccggcgtc cggctgaagg
      601 atgtgttggc caaagccaaa gcggatgcga gatccgtcgc cgtctccttc aacggcatgg
      661 atgatccgcc actcgagacc gttcccgatt ttgtcaagtc cttacccttt gcccgcgcga
      721 acgatggcga agtcatcatc gcctatgaaa tgaacggtga gcccctgccc ttgctcaacg
      781 gctatccgct caagctggtc gttcccggat ggtatgcgac gtattgggtc ggcatgctca
      841 acgacatccg cgtgtatgcc gatacgtttc acgggtactg gatggacaaa gcctaccgtg
      901 ttcccaaagg cgtgagcaac gccaacgaat cgcccgactc gctcgcgaaa gacctcgaac
      961 cgatcagccg catcgccatt cgctccatct tcgtttctcc ggaaccggac agcgtcctga
     1021 ccaagggcac gcgctacgag atggagggat tggccttcga cggcggcagc ggtatcgcgc
     1081 gcgtggagat cagcaccgat gacgggaaaa gctggaccga aacccggctc gatccttcgc
     1141 tgggcaatta ttcctggcgc cgctggcgct tcccctacac accggcggcg gcaggcaagt
     1201 tgcgcatgct ggtgcgggcc accggagtgt ccggtgaaac gcaaccgctc aaacactgga
     1261 accgcagcgg ctatgcacgc aacgagattg aatcccttga actgacggta cgctgatccc
     1321 atgacaggca cttcgcttct tcgctatccc ttcgccttgt tgctggtttt ttatgcctgc
     1381 tcacgaccgg cagaaccggc ggcccgtgtt cgcaccaccg atccgattcc gctttcagca
     1441 gtactgaccg aattacccga gcatcccgac aaaatgctgg tgatggcgaa ttgcctgccc
     1501 tgccattcgc tcgcctacat cgccaaccaa ccaccgatga aacgcgaagc ctggaagaag
     1561 accgtacgca agatgatcga cgtgttcggc gcaccggtcc ccgatacggc cgtcgcggaa
     1621 cgcattacgg attaccttgc ttcgattcgg gggagtgaat aggagcaacg aaggacttgg
     1681 tagcgttgat cgtcagtcag cgattataca aagaatcgac tctgaccgat ttgagttcga
     1741 agtaaatgcc acgatctcgc aaccaggaaa tttaataaac aagtcagcgt ctctttcaat
     1801 acgactgaag aatgactttt cctgtgagct tctcccgctc cgtcaccacc gtcacaaagt
     1861 aaatccccgc cgcccggttg tgcagggcga tgttcttgct taaatagccg gagagcaaaa
     1921 tgacggactc ctcgaccagt atcttgccgg agatgtcgga cacaaagatc ctggctcgct
     1981 ttccttccag cttgctggcg ttgaccacga tgctctccca ggctgaatga tagtaaacgc
     2041 tcagggccgg tgtgggagcg gcggtttcct ggagtgcggt ttggatcgtg tcgcaggggc
     2101 tgccggccag cgggcccaga tcgtagttgg ggttgttggg caaaccccag taggtccgct
     2161 tgccgcccag gtagaaacta tagggctgaa aatcgcaggc cgccgcaccg ccccgctgat
     2221 cgggttggtt gatgaccgac aggctcatgt tctccgggta ataatccgta ctgtcgtagg
     2281 gatagccggt tttctgggcg gccacgtaga tcttgttgtc cggagccagg cggaccgctc
     2341 cgatggtcat ggggtaggtc tgttgccaga tcagggtcct agaggcatac acattggtcg
     2401 ccgtgagcgg aaactggtac aaataaatct gcgtcgtgtc cagggaagag aaatacggcg
     2461 cgcagcttac atacagcagg gagtcgttag gggaaaaaca acagtccagg aaataaggct
     2521 gtgttcctgc agggaactcg gcatgaatgg tttggtccag ggtgatactg ccggtgcagc
     2581 gatcgaaact gtaaatggcc agcaagccat tcaaattact gaatgcgaac ttcgtgccgt
     2641 ctgaattaaa acgcaaatct ccgccattgg aagagtgaag cggccctagg ctgtcaacat
     2701 ggtccaactg cacaccattg gcatcgatcg aatagacata gaaattatta ttggaaggag
     2761 gggccgaccg atatattatc caccaatccc tgccatttcc atgtttcaca gcttgtatgc
     2821 aatcagtggc aatatcattc cgtagttgat tgtttttcga aatcaccgca ccacgaccgc
     2881 tgtctaaggt cttatcaacc agagaaaagt ataagccata ctgtccggtt acgccaatgt
     2941 gaaaaacata atactgatca ggattttccg gactgggaac gataaccatt tcatgatacc
     3001 acacttcaca attcaaggag tctccattca acatttgttc atgtcgattg ttgtaaatga
     3061 tagctttcaa tactccatta tttaaatatc taatgtcagg tgagccggaa taaaaaagca
     3121 aattcccgag actatcacta atggaggctg ctgttccttt agtattgatt ccgctttgaa
     3181 agacgacagg agagagtcta ttgcgaaagt caatccctgc actgtcccca aaacaccata
     3241 tcgtattttt accttgtcca cttgcaagga aactggaaag tgaaagcata actagtatgg
     3301 caatgctcct catttttgaa ggatcaattt attgtatgaa acaagctcat tgtcgcagta
     3361 gatttttaac aaataagtac cggactttaa accgctcaag tcttcctccc aaattgcttc
     3421 attctgattc aagctggtgc ttagtagcaa acgatcttgt aaatcattga cctcaattct
     3481 acaggaacca aagtctgcta gatcctttga tattataatt ttcccacttg aagggttggg
     3541 atagagtact agttcaggat cgggaacgtc agtcagattg ttgagctggc gaagtagcgg
     3601 ccccgtcaac tcgtcatcat actcgacatc tataatggct ctggctatga atactgccgg
     3661 gcctccttca aaagccaact gactggaaat gttatcaaga agtacggtgt cgctactgga
     3721 aaggggaagt tcggtttgaa tcttctccac caaatagagt acatctttaa aattctgctc
     3781 aatcgtgtta tccggttgaa ggctgatcaa ctttgtaata gcctgcaagt aattctcggt
     3841 gtttaacgcg ttcagaatgg cttcgatttc cccaacattt cttagtttca ggtcgtcgaa
     3901 aaattgctgg cgctgctgat cggtaggcaa tccctgattc atcagcattg aatctttcga
     3961 aagtaaatgg aatgcatact tatcagcgaa ccatttgttt tcagagttga aaacaagaaa
     4021 gactatcgaa tcattaataa tctgttcggc aatttctgag aacaattgag gaagggtatc
     4081 cgggacacta catggggaca atccggtaat gctctggatg ctttggacaa tgaaattatt
     4141 tccagggctg aacttattat tcgggtcatt ttgggtgtac cgatggtaaa attttaagaa
     4201 taatccatta gcggtactgc cgacaaccct atttgccagc gggatattcc gccacatatt
     4261 atcctgcggc gcattcacat cgccttgctg gggaatcgta atgttgaaca cgtctaaccc
     4321 tctggcacaa ttccacaggt catttccgca aaggctggtg aggtcacaga agttcaggcc
     4381 gttgatgccg ctgccaagat tcaagaggta gttgttgcgg atggagcatc ccgtggagct
     4441 ttcaaaccgg ataccgcgtg ccaatgtcgg gtcggtgacc gggctggtgg tcacgacgct
     4501 gtcgtcggtg atggtacagt tcgaactgtt caccagccag attccgtatt gccgggtggt
     4561 gctgccggcg cctttgagtt gtataaaatt ggtgttgatg tccacttcct cacagccatt
     4621 caggtaaatg cccttgagct gatccatcat gtggttattg gagacggatg ctgtcgtaac
     4681 tccgggcagc cggttttgca gttttatcga ccaattgaca gctgcgttgt ataagggcga
     4741 agtgttgagg ttagacgggc tcgaaaatgt attgtcacgg atagtcgtgc tgctggccag
     4801 gcggggctct gaaacggtaa tgccgttgag gtagtgggtg atcgagttat cgtgaatgtt
     4861 cacatctaca gcgaccggat ttacaacaga aaccgcagca tatcgcaagg agtcaaagga
     4921 tgatccggaa acggtggttt ggatgctgtt cctggcttgt actccgtacc cgcaatcggt
     4981 aaaactactg ccttgtattg taagggagac cgggctgccg gattggttca gcgagtagat
     5041 tccaaaacca tcgttttccc aggtgtagga tgccgctctt gcattgccgt aggcataagc
     5101 atggcggcgc atatggcgga aggcacaggc ctgcagggtg atcgaactgt tttctgaacg
     5161 gacaccgtaa tagagatgcc ggaactggtt ggcattaatc gtccaaccgt tcaagccgga
     5221 tgtttcaatc ccgctgtagg catatccgcc atcttttgtc tgagcaggaa ggccattgcc
     5281 gtcttcacag gtaaaggtac agccgctgac caccgagctc agcgcagctc cggagccgta
     5341 gaggacaatc gactgccggt ttctgttaaa gaccgaattg gtgactgaga tccgggaacc
     5401 ggcagaatcg gcaatcgcca tttgtgcatc ttctatcgta tcgccgctca ggatgagcaa
     5461 gccggtgctg tccgcaaagg aaatcccgcc ccacagggca ttgcaggcag aaagccggct
     5521 gttctgaatg gtcagcgtcc tgcccgggga gatgaggatg cgggagttgt cacccatcaa
     5581 caggctggag ttggtaatgg tgaagttagt atttacaacc aaatccccgg cgatactgat
     5641 cgtccggccg gtccaattgg taaaatgcga ggtgctgtcg ctgcccagga tcgtggtatc
     5701 ctgcgcagga caacactggt aa
//
LOCUS       DAOTON010000060         5673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_12034_length_5673_cov_3.515664, whole genome shotgun sequence.
ACCESSION   DAOTON010000060 DAOTON010000000
VERSION     DAOTON010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12034_length_5673_cov_3.515664"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="P5292_02030"
     CDS             <1..1178
                     /locus_tag="P5292_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRS37933.1"
                     /translation="RPEPFLQRGYDGRGLRIAVFDAGFPNVNTHPAFQHLRDRNAIVA
                     THDFVTGKENVYAHHWHGTAVLSCIAGKMDSVPLGLASGAEFLLARTESALGEPFSEE
                     EHWLEAVEWAYAHGANIISSSLGYTNKRYFNYEMDGRSSLVARAANIAASKGLLVINA
                     AGNEGTDDWRFIDTPADADSVLAIGGTEPTQDVHIGFSSYGPSSDGRLKPNLCAPGRV
                     TVAAGRGYREVQGTSFSTPLVAGFAACTWQANREKRNMEVFHLLEQSGSLFPYYDYAH
                     GYGIPQAQRALGDTASSPTFDFVVVNDEVKVVLREQFSYPETERELGYAVQRNLYYKI
                     SDALGNIRTYTVMTAENKEALHFYAGDLQNGDAVTVHFEGYTSSFDIQHDPETTPEK"
     gene            1175..1885
                     /locus_tag="P5292_02035"
     CDS             1175..1885
                     /locus_tag="P5292_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37934.1"
                     /translation="MNTRNFVTLLLLSLLAFARPASAQTMLLHVDRAVDSLPQRTGPN
                     LSRFSHLYVQAGFVAGPDEAGARIKYGNSMDFAIGFRKKYKLGSLYSFGWELELRGMN
                     YRLVQDSAKVVPNNFQNDRERLSLSVVQVGVYQRINFDPLRGNHMGTYLDLGVSAGYQ
                     VLQNNVRNKLPDGSVVNASITKVPYDNNFGAMLNARIGRGHVALYGSWRLTDVFKSSY
                     SFPELPRLTVGLEIGLFQ"
     gene            2086..2328
                     /locus_tag="P5292_02040"
     CDS             2086..2328
                     /locus_tag="P5292_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37935.1"
                     /translation="MSLSKEQKKDLDKIGQELRKLERLSVENKLDLDVFMTSYSDEGY
                     DIYATEKVSIEEQASKRKKVFEKAGKQKTEKVAKAR"
     gene            2472..4361
                     /gene="nuoL"
                     /locus_tag="P5292_02045"
     CDS             2472..4361
                     /gene="nuoL"
                     /locus_tag="P5292_02045"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRS37936.1"
                     /translation="MIDWIWLVPVFPLLGFLLISLGGKQLPKQAAGFIGSATVLASFL
                     ISLGTFLQLPAEPTTVTLFPWIHAGNFHVDFSFLVDPVSAVMLLIVTGVGFLIHVYSI
                     GYMSHDEAFARFFSYLNLFVFFMLLLVLGDNYLVLFTGWEGVGLCSYLLIGFWFKHPE
                     YNKAANKAFIMNRIGDLAFLLGIILTFVHFGSITFSEVFPIAKNAATNQPVIIAITLL
                     FFIGATGKSAQLPLYTWLPDAMAGPTPVSALIHAATMVTAGLYMVVRSNLLYSAAPFT
                     LEIIGIVGTATALFAAAIALTQTDIKKVLAYSTVSQLGYMFAALGCGAYASSLFHVTT
                     HAFFKALLFLAAGSVIHAMGGEQDIRKMGGLRKKLPITYAVFLIGTLAIAGVPPFAGF
                     FSKDEILAYMWGHNRVLFFFLAVTSVMTAFYMFRLFFLTFFGGFRGTHEQEHHLHESP
                     AAMTFPLIVLAVLSTLGGFMGLPSIFSHHHALREFLEPVAANASLMAVEPSHFFEWAL
                     IATSLVVLAIVIVYAYNRYVKRANVPSGSEQEWDQLSRWSYHKFYVDEFYDALFVRPL
                     RALGGFFGRVFDPQVLDGAVTAVSAGVNGASGLLRRVQAGGIGFYIFLMVFGIIAILV
                     YNLVL"
     gene            4377..>5673
                     /locus_tag="P5292_02050"
     CDS             4377..>5673
                     /locus_tag="P5292_02050"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRS37937.1"
                     /translation="MLSILIFLPLVAGLIAGWSGRNARTIALAAAVAEFLFSVWFALQ
                     FNATGGTQFLEDYWWVQGLGISYKVGMDGISLLLVLLTTFLTPLIILSAAGQQQERPG
                     LLYALILVMEAALIGVFCALDGFLFYVFWELALIPIYLICLIWGGKDRLRITLKFFIY
                     TLLGSLFMLVALIYLRQQTPAHSFDIRQLYALHLSPAEQTWIFWAFFLAFAIKMPVFP
                     FHTWQPDTYTDSPTQGTMLLSGIMLKMGVYGLLRWLLPIAPAGVAEWGNVAMILSVIG
                     IVYASLIAWSQQDFKRLIAYSSIAHVGLISAGVFSLTVQGFQGSVMQMVAHGVNVVGL
                     FFIAEILMRRTHTRELDHLGGIRNVAPQFATYFVILLLASVALPLTNGFVGEFLLING
                     VYQYNAWMAAVAGLTVILGAVYMLNAYRKISLGETGSRTH"
BASE COUNT         1068 a         1756 c         1573 g         1276 t
ORIGIN      
        1 ttcgtccgga acctttcctg caacgcggct acgacggtcg cggtctccgc atcgccgttt
       61 tcgacgcggg ctttccgaac gtaaacacgc atccggcctt tcagcacctg cgcgaccgca
      121 acgccatcgt ggcgacgcat gatttcgtga ccggcaaaga gaacgtgtat gcacaccact
      181 ggcacggcac ggcggtcttg tcgtgcatcg ccggaaagat ggacagcgtt ccgctcggtc
      241 tcgccagcgg ggcggagttc ctgctcgcgc gtacggaaag cgcgctggga gagccgtttt
      301 cggaagagga gcactggctg gaagcggtgg agtgggcgta tgcgcacggc gccaacatca
      361 tcagcagttc gctcgggtat accaacaagc gttatttcaa ttacgagatg gacggccgca
      421 gcagcctggt cgcgcgtgcc gcgaacatcg ccgcctcgaa ggggctgctc gtgatcaacg
      481 ccgcgggcaa cgagggcacc gacgactggc ggttcatcga cacccccgcc gatgccgaca
      541 gcgtactggc catcggcgga accgagccga cgcaggacgt acacatcggc ttcagcagtt
      601 acggtccttc gagcgacggc cgcctcaagc ccaacctctg cgcgcccggg cgtgtgacgg
      661 tggcggcggg gcgcggttat cgcgaagtgc aggggacttc gttctccaca ccactcgtag
      721 ccggcttcgc ggcctgtacc tggcaggcga accgcgaaaa gcgcaacatg gaagtgttcc
      781 atctgctcga acaaagcggc agtctctttc cttattacga ttacgcgcac ggctacggta
      841 ttccgcaggc gcaacgcgcg ctgggcgata ccgcctcctc gccgaccttc gacttcgtgg
      901 tcgtcaacga cgaagtgaaa gtggtgctgc gcgaacagtt ttcgtatccc gagaccgaac
      961 gcgagctcgg ttatgcggtg cagcgcaatc tttactacaa gatctccgac gcgcttggga
     1021 acatccgtac ctacacggtg atgaccgccg agaacaaaga ggcgctgcac ttctacgccg
     1081 gcgatctcca gaacggcgac gcggtcaccg ttcacttcga aggttatacg tcctccttcg
     1141 acatccaaca cgacccggaa acgactcctg aaaaatgaac acacggaatt tcgtcaccct
     1201 gctgctgctg agtctgctcg cgttcgcgcg cccggcttcg gcgcagacca tgctgctgca
     1261 cgtcgaccgc gccgttgatt ccctgccgca acgcaccggc ccgaacctgt cgcgcttttc
     1321 gcacttgtat gtacaggccg gcttcgtcgc cggtcccgat gaggccggcg cacgcatcaa
     1381 atacggcaac tcgatggatt tcgccatcgg ctttcggaaa aagtacaagc tgggcagtct
     1441 ctattcgttc ggctgggaac tcgagctgcg cggcatgaac taccggctcg tacaggattc
     1501 ggcaaaggtg gtaccgaata actttcagaa cgaccgggaa cgcttgagtt tatcggtggt
     1561 acaagtaggc gtctaccagc ggatcaattt cgacccgctg cggggcaatc acatgggcac
     1621 ctacctcgat ctcggcgtaa gcgccgggta ccaggtactg cagaacaacg tccgcaacaa
     1681 gctgcccgac ggcagcgtgg tcaacgccag catcaccaag gttccgtacg acaacaattt
     1741 cggcgcgatg ctcaacgcgc ggatcggccg cgggcatgtc gcgctctacg gttcctggcg
     1801 gctgaccgat gttttcaaga gctcctacag cttccccgaa ttgccgcgcc tgacggtagg
     1861 cctggaaata ggacttttcc agtaatttaa aacatcaaaa acatcttttt acactctaaa
     1921 acccatttta aacacttatt aaccgtgttt ttcaacagag ttcgcagaaa ttaacagggt
     1981 ttattaacaa gttcggttaa taacattaaa aaacccctcc aaaacaccgc aaccccagcg
     2041 ggttatatcc ggttaaattt gctgtaatct caacaaaaaa cgggtatgtc cctctccaaa
     2101 gagcaaaaga aagacctgga caagatcggc caggagcttc gcaaactcga acgattgtcg
     2161 gtcgagaaca aattggatct ggatgtgttc atgacctcgt acagcgatga gggatacgac
     2221 atctatgcca ccgagaaagt gagcatagaa gagcaggcct ccaagcgcaa gaaggtcttt
     2281 gaaaaggccg gaaagcagaa gaccgaaaag gtcgccaagg cccgctgatc ctcctgaacc
     2341 gtaagtaatt agtcaacccc cgtctgcgaa cgggggttgt tgtttcaagt cgccgggtcc
     2401 gggcccgaag cgcgccaact ggcctccaat accggattag tactactttt gccccctcat
     2461 ttgtcccgac catgattgat tggatttggc tggtgcccgt gttcccgctt ctgggattcc
     2521 tgctcatcag cctgggagga aaacaacttc cgaagcaggc ggcgggtttc atcggctccg
     2581 ctaccgtact ggcctcgttc ctgatctccc ttggcacctt tttgcaactg ccagcggagc
     2641 ccacgactgt cacgctcttt ccgtggatcc acgcgggtaa ttttcatgtc gatttctcct
     2701 tcctcgtcga tcccgtatcg gcggtgatgt tgctcatcgt aaccggcgtc ggtttcctta
     2761 ttcatgtgta ctcgatcggg tacatgagcc acgacgaagc ctttgcgcgt ttcttttcct
     2821 acctcaacct cttcgtcttc ttcatgctgt tgctcgtgct gggcgacaac tacctggtgt
     2881 tgttcaccgg ctgggaaggc gtgggtcttt gttcgtacct gctgatcggc ttctggttca
     2941 agcatcccga gtacaacaag gcggcgaaca aggcctttat catgaaccgc attggtgatc
     3001 tggcgttcct gttgggcatc atcctcacct tcgtacactt tgggtcgatc acgttttccg
     3061 aagtgttccc catcgcgaag aacgccgcca cgaatcagcc ggtgatcatc gcgatcacgc
     3121 tgctcttctt catcggcgcc acgggcaaga gcgcccagct cccgctctat acctggctgc
     3181 ccgacgccat ggcgggtccg acaccggtct ccgcgctcat ccacgccgcg acgatggtga
     3241 ccgccggcct ctacatggtc gtgcgctcca acctgcttta ttcggccgcg cccttcacgc
     3301 tggagatcat cgggatcgtc ggcacggcaa cggcgctctt cgccgcggcc atcgcgctga
     3361 cacaaaccga catcaagaaa gtacttgcgt attcgaccgt cagtcagctc ggctacatgt
     3421 ttgccgcgct cggttgcggc gcgtatgcca gctcgctctt ccacgttacg acgcacgcct
     3481 tcttcaaggc cctcttgttc ctcgcggccg gatcggtgat ccatgctatg ggcggcgagc
     3541 aggacatccg caagatgggc gggctaagga agaagctgcc gatcacgtac gcggtcttcc
     3601 tgatcggcac cctcgccatc gcgggtgttc ctccatttgc cggatttttc tcgaaagacg
     3661 agatcctcgc ctacatgtgg ggacacaacc gcgtcctctt cttcttcctg gcggtcactt
     3721 cggtgatgac ggctttctac atgttccgcc tgttcttcct caccttcttc ggcggtttcc
     3781 gcggaacgca cgagcaggaa caccatttgc acgagagtcc ggcggcgatg accttcccgc
     3841 tgatcgtgtt ggcggtcttg tcgacgctcg gcggcttcat gggtttgcct tcgatcttca
     3901 gtcatcacca cgcgctgcgc gaattcctgg agcctgttgc ggccaacgcc tcgttgatgg
     3961 cggtcgagcc ttcgcacttc ttcgaatggg cgctgatcgc cacctccctg gtggtcctgg
     4021 ccatcgtgat cgtatacgct tacaaccgct acgtgaagcg cgcgaacgtg ccttccggca
     4081 gcgagcagga gtgggaccaa ctgtcccgct ggtcgtatca taagttctac gtcgacgagt
     4141 tctacgacgc gctcttcgtg cgcccgttgc gcgcgttggg cggattcttc ggtcgcgtat
     4201 tcgatccgca ggtgctcgac ggcgcggtta ccgctgtcag tgccggagtg aacggcgcga
     4261 gcggactgct gcgccgcgtt caggccggtg gcatcggctt ctacatcttc ctgatggtct
     4321 tcggcatcat cgccattctt gtatacaacc tcgtgctgta agcgaattcc tcttccatgc
     4381 tgtcgatcct catctttctt ccactggtag ccgggcttat cgcaggctgg agcggccgca
     4441 acgcgcgcac catcgcgctg gcggcggcag tggccgaatt cctgttcagc gtctggttcg
     4501 cgttgcagtt caacgcaaca ggcggtacac aattcctcga agattactgg tgggtgcagg
     4561 gactgggcat cagttataaa gtgggcatgg acggtatcag cctcctgctg gtgttgctga
     4621 ccaccttcct cacgccgctc atcatactct ccgccgccgg acaacagcag gaacgaccgg
     4681 gtttattgta cgcgctcatc ctcgtgatgg aagcggcgtt gatcggcgtc ttctgcgcgc
     4741 tcgacggatt cctcttctat gtattctggg aactcgcgct catcccgatc tacctgatct
     4801 gtctgatctg gggtggaaaa gaccggctgc gcatcacatt gaagttcttc atctataccc
     4861 tgctcggcag cttgttcatg cttgtcgcgc tgatctatct gcgccagcaa acgcctgctc
     4921 attcgttcga catccggcag ttgtatgcct tgcatttgtc tccggcggag cagacctgga
     4981 tcttctgggc ctttttcctg gccttcgcga tcaagatgcc ggtgtttcct ttccacacct
     5041 ggcaacccga tacctatacc gattcgccca cccagggcac gatgctgctc tccggcatca
     5101 tgctgaagat gggtgtttac ggcctgctcc gctggctgct gcccatcgcg ccggccggcg
     5161 tagcggagtg ggggaacgta gccatgatcc tttccgtgat cggcatcgtg tacgcgtcgc
     5221 tgatcgcctg gtcgcagcag gacttcaagc gcctgatcgc ttattcgtcg atcgcccacg
     5281 tgggattgat ttccgccggc gtcttctccc tcacggtgca gggcttccag ggtagtgtca
     5341 tgcagatggt cgctcacggc gtgaacgtgg tcggcctctt cttcatcgcc gagatcctga
     5401 tgcgccgcac gcatacgcgc gaactcgatc accttggcgg catccgcaac gtagcgccgc
     5461 agttcgcgac ctacttcgtg atcctgctgc tggcgtcggt cgcgctgccg ttgaccaacg
     5521 gcttcgtcgg cgaattcctg ctcatcaacg gcgtgtacca atacaacgcc tggatggcgg
     5581 ccgttgccgg cctgacggtg attctcggcg cggtgtacat gctcaacgcc taccgcaaga
     5641 tctcgcttgg ggagaccggc tcgcgcacgc aca
//
LOCUS       DAOTON010000061         5636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_12125_length_5636_cov_3.691095, whole genome shotgun sequence.
ACCESSION   DAOTON010000061 DAOTON010000000
VERSION     DAOTON010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12125_length_5636_cov_3.691095"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="P5292_02055"
     CDS             <1..1011
                     /locus_tag="P5292_02055"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRS37938.1"
                     /translation="QIHHIDMHLKLLDEETLLVGESPPGIADGPQIEQNLAYVLSNYA
                     SPWGDPYRVVRIPMPPDAMGVYPNTGGDYRTYTNAVFVNRTVLLPFYDTQYDTTALRI
                     WQEAMPGHRIVGIDCNDIIPSLGAIHCITKEIASRDPLYIAHQRIRDQPAGGSGYAVD
                     AIIRHRSGIASATVWYRTDTLQPWLAVTMNLTNPSNNTWSCTIPAQASGSLVGYYIEA
                     TSNSGKQSTRPLPAPDAYWSFNVGVVTNLVATQSSPGFTLFPNPASGICLFGTELAVT
                     ETVLIRTIDLTGRVVDEQTVLIPPGPAYWQLPIDGLPSGIYTVLAEWSSGSQARKLMI
                     NR"
     gene            complement(1012..1455)
                     /locus_tag="P5292_02060"
     CDS             complement(1012..1455)
                     /locus_tag="P5292_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRS37939.1"
                     /translation="MKRHPALITLSREHHDGLVIAQVLKSDVPAYRGMPEQPADRLEY
                     FKARFVTALKPHFISEEQYLFPMLKGRDAGVDDLLNRLTEEHRELETATRLAAGDPEL
                     ESRLDATGRLLERHIRAEERELFQWAQEHLSESELQTLASHVSRS"
     gene            complement(1470..2591)
                     /locus_tag="P5292_02065"
     CDS             complement(1470..2591)
                     /locus_tag="P5292_02065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRS37940.1"
                     /translation="MIQRRYLSFSLVVACLLCLPSCKVGRFFWYNFSNITDHKIFPKR
                     DLPASTQPFRFVDAQQNDVFGRTVKVKDHSGETRALDEFLAQSPTVAFLVIRNDSLLY
                     ENYFDGYSESSTVASFSMAKSYVSALVGIAIGEGKISSEHDTVTRYLPELKGRDNWDR
                     VTLHHLLQMSSGVKFSEGYYSPFSGAATYYYGRRLRESVRHLHTDKEPMSGFDYKSVN
                     TQLLADILERATGRTVTDYLNEKIWQPIGMEYDASWSIDKKDGMEKAFCCINAKARDF
                     AKFGRLYLNGGNWNGKQLVPEAWATSSVVPVDEKGAATYYNRQWWIGSKSEGDYAAIG
                     HLGQYIYVLPKKKLIVVRLGTSRGKDEYLSIIRQVAGQF"
     gene            complement(2641..4251)
                     /locus_tag="P5292_02070"
     CDS             complement(2641..4251)
                     /locus_tag="P5292_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRS37941.1"
                     /translation="MSRRLCCLALIALVVSSCAIQVAPQGGERDTKPPVLLRADPPDH
                     TAGFHGNSIRLDFDEYVQLKDAASQFVVSPPLAQPPLIKARKKSILVSWDDTLREATT
                     YTFSFGNCIVDNNEANPVADYQMVLSTGSVIDSLTVDGSVVDAYNDNPVNGALVMLYR
                     SLPDSVPVTDLPANFSRTGSDGKFRISNVSPGKYYVLAVNDLNSNYRYDGGDERVAFT
                     DSMIEVPVSGIRLRMFREVPESKLIRGFSIVAGKAAFAFSGPASGLRWEWITDSLYVN
                     PYGWEWNLKQDSVTLWYRNQIADSMAIRFLQAGRIDTADFRLFKKAEKNQGISKKIIA
                     SDFTITSFVNGKQQRKVPFSVEFRIPVASIDSSRIRFSADSVPVKVPFHWDDAIHRKL
                     SFFSEWADDRMYRVLFLPGAFTDLFGKANDSSVVEFKVRGEREYASLRFDLRQQQPGS
                     PRLLQLVSDHGDIVREAALRQDTIVSWGFLDAGIYRVKLIDDKNENGRWDTGDLLRRV
                     QPELVRFAPEAVPLRSNWDADLRWEEGK"
     gene            complement(4248..4736)
                     /locus_tag="P5292_02075"
     CDS             complement(4248..4736)
                     /locus_tag="P5292_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRS37942.1"
                     /translation="MAESIKLPKSATRAERYELLLPQLKALLDAGVGPVANMANVAAA
                     LREAFGFFWVGFYLVDPAIERLVLGPFQGPVACTTIAKGKGVCGTAWAESRVILVPDV
                     DQFPGHIACSSDSRSEIVLPIRRQGTIVGVLDVDSDKLNDFSEVDARGLSQVLALLEE
                     CL"
     gene            complement(4771..5463)
                     /locus_tag="P5292_02080"
     CDS             complement(4771..5463)
                     /locus_tag="P5292_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRS37943.1"
                     /translation="MRILRLWTDLLWLLYPLRCAACERDLVEGEKAICTHCRYRLPRT
                     HFHHFPGNVVERHFWGKIPLVAAAACFHFSKGERVQRMIHHLKYKQRPDVGVEVGAWY
                     GRDLLQVPAFASIDLIIPVPLHPEKLRLRGYNQAACFAEGLGTGMGKTVLPDGLLKVV
                     ATSTQTRKHRFDRFRNVEHVFQAADPAGLTGRHILLVDDVITTGSTLVSCAETLLEVP
                     GVRISIVGMAAA"
BASE COUNT         1305 a         1625 c         1503 g         1203 t
ORIGIN      
        1 cagatccatc acatcgacat gcacctgaaa ctgctggatg aagagacctt gctggtcggt
       61 gaatctcctc cgggcatcgc cgacggtccg cagatcgaac aaaacctggc gtacgtattg
      121 tcgaactacg cgtcgccctg gggcgacccc tatcgtgtcg tccgcatccc catgccgccg
      181 gacgcgatgg gggtctatcc gaataccggt ggtgactacc ggacctatac gaatgccgtg
      241 tttgtcaacc gaacggtgct cctccccttc tacgacacgc aatacgacac gacggcacta
      301 cgcatctggc aggaagcgat gccgggccac cggattgtcg gcattgactg caacgacatc
      361 attccatcct taggcgcgat ccattgcatc acgaaagaga tcgcttcccg cgacccctta
      421 tacatcgcgc accaacgaat acgcgatcag ccggcgggcg gcagcggtta tgcggtcgat
      481 gccatcatcc ggcatcgcag tggcattgct tcggctaccg tatggtacag gaccgatacc
      541 ttacaaccct ggttggcagt tacgatgaac ctcaccaacc cgtcgaacaa cacctggtcg
      601 tgtacgattc ctgcgcaagc cagcggcagc ctggttgggt actatatcga agccacgagc
      661 aattcaggta aacaatccac ccgtccccta ccggcaccgg atgcctattg gagttttaat
      721 gtcggggtag tcacgaacct ggtagcgacg cagagcagtc ccggcttcac actattcccg
      781 aatcccgcgt ccggcatttg tctattcgga acggaactgg ctgttaccga aaccgtcctg
      841 attcgcacga tcgacctgac aggacgcgtg gttgacgagc agacggtact aattccgccg
      901 ggtcctgcct attggcagct cccgattgac ggattgcctt ccgggattta caccgtgttg
      961 gcggaatggt cttccggatc ccaggcacgt aaactgatga tcaatcgctg atcagctcct
     1021 gctgacatgc gacgcaagcg tctgcagctc gctttcggat aggtgttcct gagcccattg
     1081 gaataattcc cgttcttctg cgcgaatgtg ccgttccagg agtcgaccgg tagcatccag
     1141 ccgggattcc aactccggat cgccggctgc caggcgcgtg gccgtttcca gttcccgatg
     1201 ctcctctgtc aggcgattca acaaatcgtc gacgccggcg tcgcgtcctt tcagcatcgg
     1261 gaacaggtac tgttcttccg aaatgaaatg gggcttgagt gcggttacga acctggcttt
     1321 aaaatattcc agacgatccg ccggctgctc cggcattccg cgataagccg gcacatcgct
     1381 cttcagtacc tgcgcgatca ccaagccgtc gtgatgttca cgcgaaagcg ttatcaaagc
     1441 cggatgtcgt ttcatggagg ctacttgaat caaaactgtc ctgcaacctg ccggatgatc
     1501 gacaggtatt cgtctttgcc tcggctggta ccgagacgga caacgatcaa cttctttttg
     1561 ggcaacacat agatatattg gccgaggtga ccgatggctg cataatcacc ttccgacttg
     1621 ctgccgatcc accattgacg gttgtagtag gtagcagcgc ctttttcatc gaccggcacc
     1681 acggagctgg tcgcccacgc ttcaggcacc aattgtttcc cgttccagtt accgccattc
     1741 aaatacaggc gaccgaactt ggcgaagtcg cgggctttgg cattgatgca acagaacgct
     1801 ttttccattc cgtccttttt atcaatgctc cagctcgcgt cgtattccat accgatgggc
     1861 tgccagatct tttcgttgag ataatcggtc accgtacgcc ccgtcgcccg ttccaggata
     1921 tcggccagca attgcgtatt cacgctctta tagtcaaagc cgctcatcgg ctccttgtcg
     1981 gtgtgcaggt gccgtaccga ttcgcgcagg cggcgtccat agtaatacgt cgcggcaccg
     2041 gagaatggcg agtaataccc ttcgctgaac ttcacgccgg acgacatttg taaaagatgg
     2101 tgcagggtca cccggtccca attatctctg cctttgagtt ccggcagata acgggtcacg
     2161 gtatcatgtt cactggaaat ttttccttct ccgatggcga tgccaacgag cgcggagaca
     2221 tacgacttgg ccatggaaaa agaagcgacg gtcgatgact cggaatagcc gtcaaagtaa
     2281 ttctcataaa gtaaactgtc attccggatg accaggaaag cgacagtagg agattgagcg
     2341 aggaactcgt cgagcgccct ggtctcgccg gaatggtcct tcaccttgac cgtacggccg
     2401 aacacatcgt tctgttgggc atcgacaaac cgaaagggtt gggtggaagc gggcaggtcc
     2461 cgtttcggaa agatcttgtg atccgtaatg ttggaaaaat tgtaccagaa gaatcgtccg
     2521 actttacagg aaggcaggca aagcagacag gcaacgacta aggaaaacga aagataccgg
     2581 cgctgaatca tgcggtaaat ttacggccaa atcccgagca tgggaggtag ccgacgggaa
     2641 ttacttaccc tcctcccagc gcaggtcggc atcccagttg gatcggagcg gaacggcttc
     2701 cggcgcgaac cggaccaact ccggctgaac cctgcgcagc aggtctccgg tgtcccagcg
     2761 accgttttca tttttatcat ctatcagctt aacgcgataa atgccggcat cgagaaagcc
     2821 ccaactgacg atggtatcct gtcgaagggc agcttcccgg acgatgtcac cgtgatcact
     2881 caccaactgc agcaggcggg gacttcccgg ctgctgctgc cgcagatcga agcgcaacga
     2941 tgcatactcc cgttctccac gaactttgaa ctccacaacg gatgaatcgt tcgcctttcc
     3001 aaacagatcg gtaaatgctc ccgggagaaa cagtacccga tacattctgt catccgccca
     3061 ttccgagaag aacgacagct tccggtggat cgcgtcgtcc caatgaaagg gaacttttac
     3121 cggtaccgaa tctgcactga accggatgcg tgaagaatcg atgctggcga caggtatgcg
     3181 gaactcgacg gagaacggca cctttcgttg ttgtttgccg ttgacgaaac tggtgatcgt
     3241 aaaatccgag gcgatgatct ttttggaaat accctggttc ttttccgctt tcttgaacag
     3301 ccggaagtcg gccgtatcga tgcggccggc ttgcaggaat cgaatggcca ttgaatccgc
     3361 gatctgattt cggtaccaga gcgtgaccga atcctgcttc aggttccatt cccagccata
     3421 gggatttacg taaaggctat cggtgatcca ttcccatcgc aaaccggacg caggaccaga
     3481 aaacgcaaag gctgcctttc ccgcgacgat ggagaagccc cgaatcagtt ttgattccgg
     3541 gacttcgcgg aacatgcgca agcgaatccc ggaaaccgga acctcgatca tggaatccgt
     3601 aaaagcgaca cgctcatcgc ctccatcgta ccggtaattg ctgttcaggt cattgacggc
     3661 aagtacgtag tacttacccg gtgatacgtt gctgatcctg aattttccgt cggaacccgt
     3721 gcgggagaaa ttcgccggca agtcggtcac cggaaccgaa tccggtaacg aacgatacag
     3781 catcaccagc gcgccgttga cgggattgtc gttgtaggca tcgacgaccg agccatcgac
     3841 agtgagtgaa tcaataacgg atcccgtact cagcaccatc tggtagtcgg ctacgggatt
     3901 ggcttcattg ttatcgacga tacaattccc gaaactgaac gtataggtcg tcgcttcgcg
     3961 caaagtatcg tcccagctta caagaatgct ttttttccgg gccttgatca ggggaggctg
     4021 ggctaagggt ggcgatacca cgaactgact cgccgcgtct ttcagttgga cgtattcatc
     4081 gaaatccagt cggatcgagt taccatgaaa accggctgta tgatccggcg gatcggcacg
     4141 caacaagacg ggcggtttcg tatcacgctc acccccttgc ggtgcaacct ggatcgcaca
     4201 ggaagaaacg accagcgcga tcagcgctaa acagcataga cggcgactca taagcattcc
     4261 tccagcaggg cgagcacctg tgaaagtccg cgtgcatcta cttcggaaaa gtcattcaat
     4321 ttgtcactat cgacatccag gacgccgacg atcgttccct gccttcggat cggaagcacg
     4381 atttccgaac gcgaatccga gctacaggcg atatgcccgg gaaactgatc cacgtccgga
     4441 accaggatga cacggctctc cgcccaggcg gtaccgcaaa cgcctttgcc tttcgcaatg
     4501 gtcgtacaag ccaccgggcc ttgaaagggt ccaagaacga gtcgttcgat agcaggatcg
     4561 accaggtaaa agccgaccca gaagaagccg aatgcttccc gtaaggcggc ggcaacgttg
     4621 gccatgttgg ccaccggacc gaccccggca tccagcaagg ccttcagttg cggtagcagc
     4681 aattcgtacc gctccgcccg ggttgccgac ttcggaagtt tgatggattc cgccacggcg
     4741 caaagatgaa aaaaagaggg cagatagccg tcaggcggct gccatcccga cgatgctgat
     4801 ccgaacgccc ggcacttcga gcaaggtctc cgcgcacgac acgagcgtgg agccggtcgt
     4861 gatgacatca tcgaccagca ggatgtgccg accggtcaaa ccagccgggt cggccgcttg
     4921 gaaaacatgc tcgacattcc tgaaacgatc gaatcggtgc ttgcgtgttt gggttgaggt
     4981 tgccaccact ttcagaagac catccggcag aaccgtctta cccatgccgg ttccgagtcc
     5041 ttccgcaaaa caagccgcct gattatagcc acgcagccgg agcttctccg ggtgtaaagg
     5101 aaccggaatg atcagatcga tcgaagcgaa agcgggcacc tgaagcaagt ccctgccgta
     5161 ccaggctcct acctcgacgc ctacatccgg tcgttgtttg tatttgaggt gatggatcat
     5221 gcgctgtacg cgttcccctt tgctgaagtg aaaacaagcc gcagccgcca ccaatggtat
     5281 ctttccccag aagtgacgtt ccacgacatt gcccggaaag tgatggaaat gcgttcgtgg
     5341 caaacggtac cggcaatgcg tgcaaatagc cttctcccct tccaccaggt cgcgttcgca
     5401 ggcggcgcag cgcaggggat ataaaagcca aagaaggtcc gtccacaatc gaaggattcg
     5461 catgccgcaa agtgctgcat gctgcccgca ttcacatcca cttaatgcgg tttctttaaa
     5521 agcaaacggg cgatgactga tcagccatcg cccgttcgta gtatcaagaa gagcttatcg
     5581 tgcggatttg atcgcgcgca ccaccttggt ggcggtctgg ttggctactt ccacca
//
LOCUS       DAOTON010000062         5604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_12212_length_5604_cov_3.341503, whole genome shotgun sequence.
ACCESSION   DAOTON010000062 DAOTON010000000
VERSION     DAOTON010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12212_length_5604_cov_3.341503"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..560
                     /locus_tag="P5292_02085"
     CDS             150..560
                     /locus_tag="P5292_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37944.1"
                     /translation="MAIIRFVTTAKNNALDTLKTAIDAGAGAGTIKVYNGTQPTTAND
                     AITSQTLLATLTFSDPCGTTSAGTLTMSAITQDTAADATGTATWARIADSTGSTVFDV
                     DVTVTGAGGTLQFNTTNFVIGGPVLISSFAVTVP"
     gene            1187..1471
                     /locus_tag="P5292_02090"
     CDS             1187..1471
                     /locus_tag="P5292_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37945.1"
                     /translation="MEQVSEHQLEPDRFQFRSRLLLLENMKGLERSRVIFYSASQHPE
                     LLPACTTGRQEQNLPSTHRRPGIARQQEQTQRIWRFPAVVQECVAVVGQY"
     gene            1773..3998
                     /locus_tag="P5292_02095"
     CDS             1773..3998
                     /locus_tag="P5292_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37946.1"
                     /translation="MPYDDKDLGIIFPTRKGEPIGSDNERTMIDNIEGSDQIRTSVRI
                     SEDENGCKKTVRLRTRWGYPDFITEKACKSSEETSPVYMDSGIVDMFSVVPDSPPLDI
                     QSAPLYYGNTQRSYATNHKLLGRIFPPNIDADTPPVEAEPGWSFDTTRGDVVGKKEAA
                     AKCPPSMFTGKARLYAQAIYGADLKNWKWTLTIPEGLSPRLAHDNGAVLFVNSGIYRD
                     ALYIHWMISIYPDGILITKLVRDNKVDSLVTKLKDPAFAADHDKIEAYILAYSTPSTT
                     MTFVLDVPVPETQMLGYGWKFNWAGNKADIIQHTEGFPNHVSTHYRFTFLRDTNLVGP
                     NNTPEQEAARWSAVLSVVEGPVSWHNFRYAQVVASPDWLFNLLYLFGTNSGGATNCDA
                     PIYCFYNKADQLELFRYSNTNGVSGVKYMVTSQPAQWMYPVDWTSENLSDYTHFGSAG
                     LKGAEGERRVRSLSPTSTGFYFSSGTCVVAAQNYTFDRYTLSEKTIVSDGSTWATSNV
                     GHNAYEHSCAANDTGWSPYYVTTDGVQRYSSGVEIINDLGDFLGYAVGDYNQVCVIGY
                     DRYEYHGNHNENFTTLLLVPFHDAEAAYIWGNVSTNESATVSGGYCEGTNQPGFFAWR
                     ETEVFSDGSGGYTYYKYYVFAGGDGTYLAVNNTPRPELNETTDSNVISLLVTKHGNYP
                     FSPPDSMSPFFAGVPYVEKQYYTHSAAVGPVVYGHGANNLDGFPASLVLTSPPPFLGW
                     A"
     gene            3999..5585
                     /locus_tag="P5292_02100"
     CDS             3999..5585
                     /locus_tag="P5292_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37947.1"
                     /translation="MKTLPSGPFLGINNRLPDFALVTDAGRWLSDAVNVDIDNAGRAR
                     RRDGVTLVQAMTGAHSVFSTGSTTGFLVRSSVLYSFSFSPSYSETLVKILTVNDAVSY
                     TKSNGSIYYSNGTDSGRIESGIWFPWALPTPTAPTVSAILGSVHAGKYQVAVRYYNSV
                     TGEAGGISAATGIELASDGALRVTLPSATTGATHVHVLVSDLNGSQVYLHSSVATGTA
                     TLDIISVSGTTLTAEEVYAEPMPPCANLFVHMGRLCGISGSTLYYGLPYRFGYYDAAD
                     GYIGFEEDISVAIANQFGTYVVTESKTFWLMGDLGKVERISDPLPYGGQAGTAFRVPH
                     KPLVGWFGNNGFVLGDEQGQVVAATQDAVDVTPDNGTWTAVLETRGYRRVVSNGYCMN
                     LENSALSRYVDYSLTSVSGGFSTKTDGLYLLTGDQAIDAHIDLGKDDFGDSSLKRLPA
                     LYLGVSSETPMELRVTTPEHEDYRYEARSCAEEMRIQRVDPGKGLRSNWYDLSIYNTD
                     GSDFTLASVSFAPVASGRRI"
BASE COUNT         1320 a         1473 c         1524 g         1287 t
ORIGIN      
        1 atctcgacga catcgaaatc tacaaaggcg tcggcttgaa caactacgga tacacagttc
       61 caaccagcgc attcacctat tcaccaatcg ccacgccgga aaacgctttg gtttacgaca
      121 acatcacccc cgtctaaagg agaaacatca tggcaatcat ccgatttgta accaccgcaa
      181 aaaacaacgc acttgacacc ctgaaaaccg ccatcgacgc tggcgctggc gcagggacga
      241 tcaaggtcta caacggcacg cagccgacca cggcaaacga cgcgattacg tcgcagacgt
      301 tgctggcaac gctgacgttc agtgacccgt gcggcacgac atcggctggt acgctcacca
      361 tgtcggctat tacgcaggat acggcggcgg atgctacggg aactgcaacg tgggcgcgga
      421 ttgcggactc gacaggatca acggtattcg acgttgacgt taccgtcact ggcgctggcg
      481 ggacgctaca attcaacacc acgaacttcg ttatcggcgg cccggtcctg atcagttcgt
      541 tcgccgtcac cgtcccgtaa catgagctat accccgcctg tcgggaattc tgtaggcttc
      601 cagaatacag gggtagcgta tacgccccct gctggggatg ctatagcttt ctcgtcagga
      661 gcagccggaa gcgccgcagc agagttaact ttctctgcaa caagttccgg tgttcatggc
      721 gtaacaggag cgggcgctgc gcagtttcta cttacggtaa gcgcggatgg gatatgcgcg
      781 ccaaaagggc ggtttgaatc tgccgaactt gcgcttgttg tttctgctga tgggtacacg
      841 gactataacg gaaacgcgtc ggcagagatt tcgttcgtag cggcatcaac cgggagtgtg
      901 cgcccggtag gggccatagc aacacgactc gccttggttg caagtgctgc cggagcaggg
      961 aacgctgcta ctggagaact cgccacatcc cttgtcatgc gggcagtcgg caccgggttc
     1021 aattcttccc caggcacttg tgtggcaacg ctcggattcg cagcagatgc aactggatac
     1081 cgtgctccca tagggggttc agccgcgcag gtacgattct cagggatagc tacaggcgcg
     1141 cacgcacaaa caggtattgc aaccaacgca ctacggtttt ccgctgatgg aacaggtatc
     1201 agagcaccaa ctggaaccgg atcggtttca attccgttcg cggcttctgc ttctggaaaa
     1261 catgaaaggc ttggaacggt cgcgtgtgat cttttattcg gcatcacagc atccggagtt
     1321 gttgccggcg tgtacaacgg ggaggcagga gcagaacttg ccttcgacgc atcgtcgtcc
     1381 gggtatcgcg cgccaacagg aacaaacgca gcgaatctgg cgttttccgg cagtggttca
     1441 ggagtgcgtg gcagtagtgg gccaatatta gcatcgctgc gttttgcttc atcaggaacg
     1501 ggcaaaacgg gcagaaccgg atcgtgctcg gcctctatct cgttcgattc cagtgttgct
     1561 ggcgggcatg gtggcagtgg atcggcatcc ggagacttga tgttcggagc aaccgcatcc
     1621 ggaatggttg cttcagtgca tagcggatcc ggtgacgctg gcttaggttt ctcttgttca
     1681 ggcgacggaa ccttgccaaa tcaacttcca tcggttccgg acgaattagt ctttgtaaca
     1741 accagggttc gcccaatcat cgcgcgtacc tgatgccata cgacgacaaa gacctcggaa
     1801 ttattttccc tacaaggaag ggtgaaccta ttggctcgga taacgagcgc accatgatcg
     1861 acaacattga agggtcagac cagatcagaa ccagcgtccg gatctcagaa gatgaaaacg
     1921 gatgcaagaa aaccgttcgg ctgcgtacca ggtggggtta tcctgatttc attaccgaga
     1981 aggcttgcaa gtcaagcgaa gaaaccagcc ctgtttacat ggactctgga attgttgaca
     2041 tgttttctgt cgttccagat agtccgcctc tcgatattca gtcggcgcca ctttactacg
     2101 gcaacacgca acgctcctat gccacaaacc acaagctgct tgggcgaatc ttccctccga
     2161 acatagacgc tgatacgccg cctgtagagg ctgagcctgg ttggagcttc gatacaaccc
     2221 gtggtgatgt tgtggggaag aaagaagcgg cagcgaaatg cccgccgagc atgttcaccg
     2281 gaaaggcccg gctctacgct caagccattt atggcgcaga cctcaagaac tggaaatgga
     2341 cactcacgat tccggaagga cttagcccga gactggctca cgacaatggc gctgtcctgt
     2401 tcgtcaatag tggaatctac cgggatgcgt tatacatcca ctggatgatc tccatctacc
     2461 ccgacgggat tttgatcact aaactcgtcc gtgacaacaa ggtggattcg cttgtaacaa
     2521 agctgaaaga tcccgcattt gccgcagacc acgacaagat cgaggcgtac attctggcgt
     2581 actcaacacc gtcgaccacc atgacgttcg tgctggacgt gccggttcct gaaacgcaga
     2641 tgcttgggta cgggtggaag ttcaattggg ccggtaacaa ggctgacatc atccagcaca
     2701 cagaaggatt tccgaaccac gtttcgacgc actaccgatt tacgtttttg agagatacca
     2761 atcttgtggg accaaacaac acgccggagc aggaagcggc aagatggtct gctgtactct
     2821 ctgttgtaga aggtccggta tcttggcaca acttcaggta tgcacaggtg gttgcttcac
     2881 ctgactggct gttcaacctt ctttacctct ttggaacaaa ttccggaggg gcaacaaatt
     2941 gtgatgcgcc aatctattgt ttctacaaca aggcagatca gcttgaactg tttcgctact
     3001 cgaacacaaa cggtgtttcc ggagtcaagt atatggttac gagtcagccg gcgcaatgga
     3061 tgtatccggt tgattggaca tctgaaaatc ttagtgacta tacgcatttt ggatctgcgg
     3121 gacttaaagg agcagaaggc gaacgtcgag tacggtcctt gtccccaaca tccacggggt
     3181 tttacttttc ttcaggaacc tgtgttgttg ctgcgcagaa ttacactttc gacaggtaca
     3241 cactcagcga aaaaacaata gtttcggatg gaagcacatg ggcaacaagc aatgttggtc
     3301 ataatgccta tgagcattct tgtgccgcca atgacaccgg atggtccccg tattatgtga
     3361 caacggacgg cgtacaaaga tattccagcg gcgttgagat tatcaatgac ttgggcgatt
     3421 ttcttggtta tgctgtcggc gactataacc aggtgtgtgt catagggtat gacaggtacg
     3481 agtaccacgg gaaccacaac gaaaacttca caactctcct tttggttccg ttccatgacg
     3541 cagaagccgc gtatatatgg ggaaatgttt ctacgaatga atcagcgact gtatccggtg
     3601 ggtactgcga aggcaccaac caaccggggt ttttcgcatg gcgtgaaacc gaagtattct
     3661 ctgacggcag cggtgggtat acatactata aatactacgt atttgcgggt ggcgatggaa
     3721 cgtacttggc tgtaaacaat actccgcgac cggagcttaa cgaaacaaca gattccaatg
     3781 ttatcagttt gcttgtgaca aaacacggca actatccgtt ctctccaccg gattctatgt
     3841 cgccattctt cgctggcgtg ccgtatgtcg agaagcagta ttacacccat tcagccgcag
     3901 ttggccccgt tgtttacggg cacggcgcca ataatctaga cgggttccct gcctcactgg
     3961 tgcttacttc tccaccacct ttcttgggat gggcataaat gaaaacactg ccttcaggtc
     4021 cgttcctcgg aataaataac cgccttcctg actttgccct ggtcactgat gccgggcggt
     4081 ggctgagtga tgcggtcaat gtggacatcg acaacgctgg ccgagcgcgg cggcgcgatg
     4141 gagttacgtt ggtccaggcg atgaccggag cgcattcggt attctcgacc ggctcaacaa
     4201 ccggctttct cgttcgttcg agtgttctct acagcttctc gttttcccct tcttacagtg
     4261 aaacgctcgt taagatactg acggtgaatg atgctgtcag ctacacgaag tccaatggga
     4321 gcatttacta ctccaacgga acggattccg gtcgcattga aagcgggatc tggtttccgt
     4381 gggcgcttcc tactccaaca gccccgactg tatcggccat cttgggttca gtgcatgccg
     4441 ggaaatacca ggttgcggtg cgttactaca acagcgtcac tggcgaggcc ggagggatct
     4501 ctgctgcaac aggaatcgaa cttgcttcgg atggtgcgct gcgcgtcacg cttccctctg
     4561 cgacaactgg cgcaacgcat gttcatgtat tggtatccga cctgaacggg tcgcaggttt
     4621 atctccactc ttctgttgcc acggggacag caacgctgga cattatttcc gtctcgggca
     4681 cgacgctgac cgcagaagaa gtttatgccg agccgatgcc gccatgtgcc aacctgtttg
     4741 tccatatggg gcgcctgtgc ggaatctcag gctctaccct ctactatgga cttccatacc
     4801 gattcggtta ctacgatgcg gcggacgggt atatagggtt cgaggaagat atttccgtgg
     4861 cgattgccaa ccagttcggg acatacgtcg tgacagaatc caagacattc tggctgatgg
     4921 gtgatctggg caaggtagag cggatcagtg acccgctgcc ctatggcggt caagccggaa
     4981 ccgcattccg tgttccgcac aaaccgcttg tcggatggtt cgggaataac gggtttgtcc
     5041 ttggcgacga acagggccag gttgttgccg caacacagga cgccgtggac gtaacgcctg
     5101 acaatggcac gtggaccgct gtccttgaga cacgcgggta tcgtcgtgtc gtatcaaacg
     5161 gatactgcat gaacctagaa aacagcgcgt tatccaggta tgtggattat tcgctgactt
     5221 cagtttccgg aggcttttcc acaaaaacgg acgggctgta cctgctgaca ggagatcagg
     5281 caattgacgc ccatatcgac cttggaaaag acgactttgg agactcgagc cttaaacgtc
     5341 ttccggcgct ttatctcggg gtgtcgtcgg aaacgccgat ggaattgcgc gttaccactc
     5401 cggaacatga agattacagg tatgaagcgc gctcctgtgc cgaagaaatg cgaattcaaa
     5461 gagtcgatcc aggcaaggga ttgcgctcta attggtacga cctgtcaatc tacaacacgg
     5521 acgggtctga cttcacgctg gcttcagtca gctttgcgcc ggtcgcttca ggcaggagga
     5581 tttaattatg cacaaacaca ggaa
//
LOCUS       DAOTON010000063         5595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_12237_length_5595_cov_2.794404, whole genome shotgun sequence.
ACCESSION   DAOTON010000063 DAOTON010000000
VERSION     DAOTON010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12237_length_5595_cov_2.794404"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="P5292_02105"
     CDS             complement(<1..838)
                     /locus_tag="P5292_02105"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRS37948.1"
                     /translation="MFTRGTLRNLRYLWSLAPGLVTIAGNALGGAWSWANLVFSLGLL
                     AALEWFVAEDHHNESASTSFLPDLILILHAVFQVVALGALFDSIAGGRIAGIHLIGAA
                     LSTGVHSGSSSIVIAHELVHRKHLAWQALGKFLLFTAGNIYFFVEHLRVHHKWVGTPR
                     DPATARFGENLYAFFLRSLSGQLSGAIRLENQRLRHEGKSTVHPDHYVWRNFTGLLLV
                     SIVCWYFLGWPGLGAWALQILTANFLLEYTNYIEHYGLSRREDERVRETHSWQTDKVV
                     SRF"
     gene            948..1640
                     /locus_tag="P5292_02110"
     CDS             948..1640
                     /locus_tag="P5292_02110"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRS37949.1"
                     /translation="MPKKYGFPAILALLLFTLGTKTAVAQSNRLMPDDLVQFSGVVVS
                     NDSLMPIPFSSIMIKNTSRGTISDYYGFFSFVAKMKDTVEFAAIGFKKAVFIIPDTIS
                     DHRCSLIQLLRRDTVLLKEVVIFPWPTKEQFKDAFLQLRVPDDDLARAERNLDRNQLN
                     ILATAMPMDGSMNFRYQMEQQSSRLYYAGQLPPNNLLNPVAWSKFIQMWQNGDFRNKN
                     KNSRKTTDDDDN"
     gene            1624..4155
                     /locus_tag="P5292_02115"
     CDS             1624..4155
                     /locus_tag="P5292_02115"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRS37950.1"
                     /translation="MTTIIKRLSAAILLGFLLLLLPGSILAQNTGVLRGTVRDSVGKP
                     LFGVTVAVFGKPQGTTTRDDGRYELSLPANEALRIVFSSTGLRTDTVLVTLTPGESRV
                     LDRRMRSRVVQFKEVNIEDRSLKTINVTPINPKVVSVLPTPNQSVEDLIKTMPGVGSA
                     NELSSTYSVRGGNYDENLVYINDFEVYRPLLVRSGQQEGISIINPDMVESIFFSAGGF
                     DAVYGDKLSSVLDIRYKKPKEFGISASASMLGGSLTIQNRSKNDKWYWMASARQRSNQ
                     YLLNTFETKGEYRPSFTDIQLLTGFEPSSRWTIELFTNYARNRYQLTPSTRETNFGTI
                     NDAKRFTVYFEGQEFDRYQTGSASASVAFRPNERVKLKLIASGFATREEENFDILGQY
                     FLDQLEADLGKDDFGDVAFNLGVGSFLNHARNRLEGQATSLEHKGERVGERSLWQWGA
                     KVSTERIEDKMHEWNYNDSAGFSIPSPRDSADPRILLNDVVIARNTLESNRVMGFLQH
                     TWQLSDTNRVILTTGIRANYWDLNEQLVISPRASITYKPDWKRNMSFRASGGVYYQPP
                     FYRELRDLDGTAYPKTKAQQSIHAVLGGDYTFLALGREFKLTTEVYYKFLENLIPYKE
                     DNLRLRYLPTLQSKGYARGIDFRLFGEFVPGTESWASLSILQTEEDITGDAYYLRYNA
                     AGEQIIAGYTFDQNAVDSQKVEPGYIPRPADQRVNFGLFFQDYLPKSPTFKMSLNLLF
                     GTALPFGPPGRDRYKDVYRSPSYRRVDLGLSKQIIGEDVVNKPQGKFFSGFKSLWIGV
                     EVFNLLQVSNVASYSWITDVSNARKYAVPNYLTARQLNLRINARF"
     gene            4155..4559
                     /locus_tag="P5292_02120"
     CDS             4155..4559
                     /locus_tag="P5292_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37951.1"
                     /translation="MDARALLTSLLLTVSLLAGPSASHGQSVVFCEQVSSDGKPVNAS
                     DYFSIKRDGGFLHVLVTLPHAVNTDQVRADLFRREKGASVFESSVYIPTSPSYRWFSH
                     RLTFYQEGEYEIFFYDKQEKLLAVGGIRLQVR"
     gene            complement(4562..5377)
                     /locus_tag="P5292_02125"
     CDS             complement(4562..5377)
                     /locus_tag="P5292_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS37952.1"
                     /translation="MPDRNRPVVVITGASSGIGKALAFEFGRNGFAVSISARQADRLG
                     KTVNELQAAGIPVYAVTGDVAKEQDCKNLIQETVNTFGRIDVLINNAGMSMRALFEKT
                     DLSVIRQLMDINFWGTVYCSKFALPYLLKQKGSIVGISSIAGKKGLPGRTGYSASKFA
                     MEGFLETLRTENLKKGLHILVACPGFTASNIRVTSLGADGKGQGESPREEGKMMQPEE
                     VARRIFNAVKQRKRDLVMTREGKLTVFLNKFFPGWLDGVVYNHMAKEPDSPFK"
     gene            complement(5370..>5595)
                     /locus_tag="P5292_02130"
     CDS             complement(5370..>5595)
                     /locus_tag="P5292_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRS37953.1"
                     /translation="DEAWQREVPKGIREIFELCVSLGGTISGEHGIGYVQKQYMDIPF
                     DPVQLELMKGIKRLFDPNGILNPGKIFPDA"
BASE COUNT         1346 a         1630 c         1396 g         1223 t
ORIGIN      
        1 caaatcgact gaccactttg tccgtctgcc agctgtgcgt ttcccgaacc cgctcgtctt
       61 cccggcggga aaggccatag tgttcaatgt agttggtgta ttcgagcagg aaattcgcgg
      121 ttagtatctg caaggcccag gcacccagac cgggccaacc caggaaatac cagcacacga
      181 tggaaacaag caacaagccc gtgaagtttc tccagacata atgatcagga tgaaccgttg
      241 atttaccttc atgcctaaga cgctggtttt ccaatcgaat agctccggag agctgaccgc
      301 tgagcgatcg caagaagaat gcgtacaggt tctcgccaaa gcgggcagta gcagggtccc
      361 tgggtgttcc gacccatttg tggtgcaccc gcaggtgttc aacgaagaag tagatgttgc
      421 ctgctgtgaa aagcaggaac ttccccaagg cctgccaggc caggtgcttt cggtgaacca
      481 attcatgggc gatgacaatc gaagaagatc ccgaatgaac gcctgtagat agcgcggccc
      541 cgatcaggtg aataccggca attctgccgc cggcaatgga atcgaacaac gcgcccaacg
      601 ccactacctg aaaaacggca tgcaaaatca ggatcagatc cggtaaaaag gaggtggatg
      661 ctgactcatt gtgatgatcc tcggcgacga accactcgag ggctgccagc aatcccaggg
      721 agaaaaccag gttcgcccag gaccaggcac ctcccaacgc atttccggca atggttacca
      781 gtccgggcgc aaggctccag agataccgca gattgcgcag ggtcccgcga gtaaacatgt
      841 acaacaaatt tacgaaaacg accggggcta aagcgttgat aaaccgtcaa ttttttaccg
      901 gaattgccgt taatcaactt cccattagtt aataccttta ccccactatg cctaaaaaat
      961 acggcttccc ggccattttg gccctgttac tcttcacgct tggcacaaag accgcagtgg
     1021 cccagtctaa ccggctgatg cccgatgacc ttgtccagtt ttccggagtc gtagtcagca
     1081 acgacagcct gatgcccatc cccttttcga gcatcatgat caagaatacc agccggggaa
     1141 ccatctctga ttactacgga ttcttttcgt tcgtcgccaa aatgaaagac accgtggaat
     1201 tcgcggccat cggcttcaag aaagcggtct tcatcatccc ggatacgatc agtgaccacc
     1261 ggtgctcgct catacaactg ctgcgacgcg atacggttct cctgaaagag gtcgtgatct
     1321 tcccctggcc gacgaaggaa caattcaagg acgccttcct gcagcttcgc gtaccggacg
     1381 acgatctggc ccgtgccgaa cgaaacctgg accgtaatca actcaacatt cttgcgaccg
     1441 ccatgccgat ggatggctcc atgaacttcc gctaccagat ggaacagcaa tcgagccgct
     1501 tgtactatgc cgggcagcta ccgccgaata acctgctcaa cccggtcgcc tggtccaagt
     1561 tcatccagat gtggcaaaac ggcgatttta gaaacaagaa caagaatagt cgcaagacca
     1621 cggatgacga cgataattaa acggctttcc gccgctattc tgctcggttt cctcctgctt
     1681 ttactcccgg gcagcatcct ggcccagaac acgggcgtat tacgcgggac ggtacgtgac
     1741 agtgttggta aaccgctctt tggcgttacg gtcgcagtct ttggcaaacc tcagggaacg
     1801 acgacacggg atgacggccg atatgaactg tcgcttccgg cgaacgaagc gctgcgcatc
     1861 gttttcagca gcacaggact ccgtaccgac acggttctgg tcacgctgac gccgggggaa
     1921 tcccgggtgc tggaccgccg gatgcgttca cgtgtcgtgc aattcaaaga ggtcaacatc
     1981 gaagaccgat cgctcaagac gatcaacgtt accccgatca atccgaaagt cgtatccgtg
     2041 ctcccaacgc cgaatcagag cgttgaagac ctgatcaaaa cgatgcccgg tgttggttct
     2101 gccaatgaat tgagttccac ctattccgtg cgcggcggca attacgacga gaaccttgtt
     2161 tacatcaacg acttcgaagt ataccggccc ttgctcgtac gctccgggca acaggaaggg
     2221 atcagtatca tcaatccgga tatggtcgaa tccatcttct tctctgccgg cggattcgat
     2281 gctgtctatg gcgacaaact gagtagcgta ctcgatatcc ggtacaagaa accgaaggaa
     2341 ttcggaataa gcgccagcgc cagcatgctg ggcggttcgt tgaccattca gaaccgaagc
     2401 aaaaacgaca aatggtactg gatggcgagt gcccgccagc ggtccaatca atatctgctt
     2461 aacacgttcg agacaaaagg tgaataccgg ccttccttca cggacattca attgctgacg
     2521 ggatttgaac ccagctcgcg ctggaccatt gaattgttca ccaactatgc ccggaaccgt
     2581 taccagctca ccccctccac gcgggagacg aatttcggca ccatcaatga cgcgaaacga
     2641 ttcacggtct actttgaagg acaggaattc gatcgctacc agaccggatc ggcttcagcc
     2701 tccgtcgcct ttaggccgaa tgagcgcgtc aagcttaaac tgatcgccag cggattcgcc
     2761 acacgcgaag aagagaactt cgacattctc ggacaatact tcctggatca gttggaagcg
     2821 gaccttggca aagacgattt cggagacgtc gccttcaacc tgggtgtcgg ctccttccta
     2881 aaccacgcac gcaaccgtct ggaaggtcag gcaaccagtc tggagcacaa aggtgagcgc
     2941 gtgggcgagc gctcgctctg gcaatgggga gcgaaggtca gcacagagcg gatcgaagac
     3001 aagatgcacg aatggaacta caacgattcg gcaggctttt caatcccctc tccgcgcgat
     3061 tccgccgacc cgcgcattct gctgaacgac gtggttattg ccaggaacac gctggaatcg
     3121 aaccgggtca tgggcttcct gcaacatacc tggcaactct ccgataccaa ccgcgtcatc
     3181 ctgaccaccg gcattcgtgc caattattgg gacctgaacg aacaactggt gatcagcccg
     3241 cgcgccagca tcacctataa gcctgactgg aaacgcaaca tgagtttccg ggcttccgga
     3301 ggagtctatt atcagccccc cttctaccgg gaactgcgcg acctggatgg aaccgcctac
     3361 ccaaaaacga aagcacagca atcgatccat gcagtactcg gtggcgatta taccttcctg
     3421 gcactcggcc gggagttcaa actcaccacc gaagtctatt acaagttcct tgaaaacctg
     3481 atcccgtaca aggaagacaa cctgcgactc cgctacctgc cgaccttgca gtcaaaaggt
     3541 tatgcgcgcg ggatcgactt ccggttgttc ggtgaattcg taccgggaac cgaatcctgg
     3601 gccagcctct ccatcctcca aacggaagag gacatcaccg gtgatgctta ttacctccgg
     3661 tataacgcgg cgggcgaaca gatcattgcc gggtacacct ttgaccagaa tgcggtcgat
     3721 tcccaaaaag ttgaaccggg ctacattccg cggccggctg atcaacgcgt caatttcggt
     3781 ctcttcttcc aggattacct gcccaagtcg ccgaccttca agatgtcact caacttgctc
     3841 ttcggcactg cactcccctt cggccctccc ggacgtgatc gttacaaaga cgtttaccgt
     3901 tcaccctcct accgacgcgt cgacctgggc ctgtcaaaac agatcatcgg cgaagacgtg
     3961 gtaaacaaac cgcaaggaaa gttcttttcc ggtttcaaat ccttatggat cggtgtcgaa
     4021 gtgttcaatc tcctgcaggt ctccaatgta gcgtcctact cctggatcac cgatgtcagc
     4081 aacgcccgga aatatgctgt gccgaattac ctgacagcac ggcaactcaa cctccgtatc
     4141 aacgctcgct tctgatggat gcacgcgcac tgcttaccag tttacttctg accgtctcgc
     4201 tgctggcagg cccatcggcc tcccacggac aaagcgtcgt attctgcgag caggtgagtt
     4261 cggacggaaa gccggtgaat gccagtgatt atttttccat caagcgcgat ggcggttttt
     4321 tgcatgtgct ggtgacgctt ccacatgcgg tgaataccga ccaggtacgc gcggatctct
     4381 ttcgtcgcga gaaaggcgca tccgtttttg agagttcggt gtacatcccg acctctccgt
     4441 cctaccgatg gttctcccat cggctgacct tttatcagga aggcgaatac gagatcttct
     4501 tctacgataa acaggaaaaa ctcctggcgg tcggcggcat ccgtttacag gtccgctgag
     4561 cttatttgaa aggggaatca ggctccttgg ccatgtggtt ataaaccacc ccatccagcc
     4621 atccgggaaa aaacttgttc aggaagaccg tcagcttacc ctcgcgggtc atgaccaggt
     4681 cgcgtttgcg ctgctttact gcattgaaaa tcctgcgagc aacttcctcg ggttgcatca
     4741 tctttccttc ctcccgggga gattccccct gccctttacc gtcggctccc agcgaggtga
     4801 cccggatgtt ggaggccgta aagcccgggc atgccacaag tatatgcagg cctttcttca
     4861 agttctctgt tcgcagggtc tccagaaatc cttccatggc aaacttggag gcggaataac
     4921 cagtcctgcc gggtagcccc ttcttaccgg caatggatga tataccgaca atgctgccct
     4981 tttgcttcaa caggtagggt aacgcgaact tggaacaata aacggttccc caaaagttga
     5041 tatccatcaa ttgtcggata accgacaggt ccgtcttttc gaataaggca cgcatcgaca
     5101 tcccggcgtt gttgatgagc acgtcgatcc ggccaaaggt gttgaccgtc tcttgtatca
     5161 ggtttttaca atcctgctct ttcgctacat ctccggtaac cgcgtacacc gggattccgg
     5221 ctgcctgcaa ttcgttaacg gtttttccca gccggtccgc ctgccgggcg ctgattgaaa
     5281 cggcgaaacc attccgtccg aattcaaacg cgagcgcctt cccgattccg gaagaagcgc
     5341 cagtgatcac gacaacagga cgattacgat caggcatctg gaaagatctt tcccgggttg
     5401 agtattccgt tcgggtcgaa cagacgtttg atccctttca tcagctccaa ttgcaccgga
     5461 tcgaacggga tgtccatgta ctgtttctgt acgtaaccga ttccgtgttc accagaaatc
     5521 gtaccgccca acgaaacgca taattcaaaa atctcccgaa ttcctttcgg cacttctcgc
     5581 tgccaggctt cgtcc
//
LOCUS       DAOTON010000064         5569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_12290_length_5569_cov_3.497280, whole genome shotgun sequence.
ACCESSION   DAOTON010000064 DAOTON010000000
VERSION     DAOTON010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12290_length_5569_cov_3.497280"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="P5292_02135"
     CDS             complement(<1..706)
                     /locus_tag="P5292_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRS37954.1"
                     /translation="MPGNSNGKNRTMGNSFEVTGGTVLQGELVPQGAKNEALQVICAT
                     LLTPEKVIIRNIPDIRDVNKLIELLQHLGVHVEQLGVLKGEFSFEAKNINLDFLETPE
                     FKKQAAALRGSIMIVGPLLARFGRARIPKPGGDKIGRRRLDTHFIGFQQLGATFEFLE
                     GDSFYTVEARKLRGTYMLLDEASVTGTANIVMAAVLAEGTTTIYNAACEPYVQQLCNM
                     LNRMGARISGLDTNLLV"
     gene            complement(812..1771)
                     /locus_tag="P5292_02140"
     CDS             complement(812..1771)
                     /locus_tag="P5292_02140"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRS37955.1"
                     /translation="MSPQDFRFSNEPEPHKQRTRAILTSHPEVRELIGKNPTTFLAIL
                     FCVGSMVALAWLVRDAAWYWVLLLAYFVGAFFNHSLFVLIHECSHNLLFKKKSWNYLA
                     SILANLPHGLPSAVSFTRYHIIHHSHQGEFLWDADLPGRKEAAWFGNNPFSKALWLLL
                     FPFFQLFRTFRLKHIRPLDGWIVTNWIVQFAFDAFIWIVFGPQALLFLLLSFFFSVGL
                     HPLGARWIQEHYLTLDPVQETYSYYGPLNTIAFNVGYHNEHHDLPSVPWNRLPELRKR
                     APEFYNTLKAHRSWTLLFFRFLFDPSITLHSRMLRADRTPGQD"
     gene            complement(1792..2481)
                     /locus_tag="P5292_02145"
     CDS             complement(1792..2481)
                     /locus_tag="P5292_02145"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HRS37956.1"
                     /translation="MAKSVRTTQRPPVDTIEYNTQRDRLIISEYGRAIQKMVQHCVEI
                     PDRDLRTRVAASIVNAMAVLNPQLKETADYKQKLWDHLFVMSDFKLDVDAPYPVPERE
                     AMRLQPKRIAYPSGDIRYKYYGKTTQEMIEKIGEMEEGQQREIITRNLANFMKMSYLT
                     WNKDTVDDETIFNHLEDLSDGKLRMSESARLNHTAEILALSKEKVRESAGTNKNNKGG
                     RMMMMKKKRKK"
     gene            complement(2575..4950)
                     /locus_tag="P5292_02150"
     CDS             complement(2575..4950)
                     /locus_tag="P5292_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRS37957.1"
                     /translation="MQHYLDELNEPQRAAVTHTDGPVMIIAGAGSGKTRVLTYRITHL
                     LHSGVDPFNILALTFTNKAAREMRERISKVAGTEARNLWMGTFHAVFAKILRIEAERL
                     GYPSNFTIYDTDDAKSLMKDILREQGLDDKVYKPDVVLNRISAAKNNLYSWMEYHQNT
                     DFIADDNSSGKPKMGLLFELYVKRCFKSSGMDFDDLLYNMYVLIQKFPDVLHKYQHKF
                     RYILVDEFQDTNYAQYVILKKLAKTFENICVVGDDAQSIYAFRGANINNILSFERDFP
                     DLNVFKLEQNYRSTKNIVHAANSIIAKNERQIRKEVWTENEIGEKIQLLRALSDNEEG
                     VMVASDIFNTKMNRQWRNKDFAILYRTNAQSRAMEEALRKGNIPYRIYGGLSFYKRKE
                     IKDLLAYFRLVINFNDEEALKRIINVPARGIGKTTIEKILLVAAEKDTNPWSLIAEPS
                     ANPCGFNAGTLNRINEFVTMIRSYAARVKTTDAYELASQLSSASGLMRELYADKTPEG
                     LSRYENVQELLNGIREFTESGRSITPDGDEVVPGTHPELRTLEQFMQDIALLTDADDD
                     EKANEDSVSLMTIHAAKGLEFPAVYVVGLEENLFPSQMSLDSRDDLEEERRLFYVAVT
                     RAEKKLTISYANSRYRWGNLISCEPSRFIEEIDPQFLEEGAVKNLRPKPRYQDDFLED
                     RWERPGLRNGGNSKPVYGTAPKNKPSAPVPPPRTVPTNLKKVNTAEVRDFEGDDTSQI
                     QQGMEVEHPKFGRGKVMLIEGRENDRKATVFFQGVGNKQLLLKFAKLRILG"
     gene            5257..>5569
                     /gene="rpsP"
                     /locus_tag="P5292_02155"
     CDS             5257..>5569
                     /gene="rpsP"
                     /locus_tag="P5292_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRS37958.1"
                     /translation="MPAKIRLTRHGKKHNAYYHIVVADGRAPRDGKFIEVIGNYNPNT
                     NPATINLDATKALNWLNKGAQPTDTCRAILSYKGVLYRNHLNKGVLKGALTAEQADAK
                     FE"
BASE COUNT         1273 a         1473 c         1474 g         1349 t
ORIGIN      
        1 tgaccaagag attggtgtcc agccccgaga ttctggcgcc catccggttg agcatgttgc
       61 ataactgctg aacatagggt tcgcaggccg cattgtagat ggtcgtcgta ccctccgcca
      121 gcacagcagc catcacgatg ttcgcggtcc cggttaccga tgcttcatcg agcaacatat
      181 aggtgccccg gagtttcctg gcttcaacgg tatagaagct gtcgccttcc aaaaactcaa
      241 aggtcgcacc cagttgctgg aaaccgatga agtgcgtatc cagacggcgg cgaccgattt
      301 tatctcctcc cggcttagga atgcgggcac gaccaaagcg ggccagcaaa ggtccgacga
      361 tcatgatgga cccgcgcaag gctgcggctt gtttcttgaa ctcaggcgtc tcgagaaaat
      421 caaggttgat gttttttgct tcaaaggaaa attccccttt gagtacaccc agttgctcca
      481 catgcacacc caggtgctgc aacagctcga tcagtttgtt cacgtcccgt atgtccggga
      541 tgttgcgaat gatcactttc tccggggtga gcaatgtcgc acagatcact tgcaaggctt
      601 cgttcttcgc gccctgaggt actaattctc cctgcagtac tgtcccgccg gtgacttcaa
      661 aactatttcc catcgttcgg ttctttccgt tgctgttccc cggcatgggg aaccacgcta
      721 cgaaataaga accggtgcgg tatcccgcgg ttcacactgc tgaatagttt taaacaaccc
      781 gcatctgaca gcgggaaagc cagcgccagg atcagtcctg accgggtgtg cgatcggcgc
      841 gcaacatccg ggaatgcagc gtgatcgacg gatcaaagag aaagcggaaa aagagcaggg
      901 tccaggaccg atgtgctttg agggtgttgt agaattccgg cgcacgtttc cgcaattccg
      961 gtaagcgatt ccagggaacc gacggcagat cgtggtgttc gttgtgatag cctacattga
     1021 acgcgatcgt attgaggggt ccgtagtagc tataggtctc ctgcacggga tcaagtgtaa
     1081 gatagtgttc ctgaatccag cgggcaccca ggggatgcag accaacggag aagaagaagc
     1141 tcaacagcag aaataaaagc gcctgaggac cgaatacgat ccagatgaaa gcatcgaagg
     1201 cgaattggac gatccaattt gttacgatcc atccgtccaa agggcgaatg tgcttcaatc
     1261 tgaatgtacg aaacaattga aagaagggga acaagagcaa ccacagcgct ttcgaaaagg
     1321 gattgttacc gaaccaggcg gcctccttgc gtccgggcaa atcggcatcc catagaaatt
     1381 caccctggtg cgaatggtgg atgatatgat accgcgtgaa cgacacggca ctgggcaacc
     1441 cgtgcggcag gttagcgaga atggaagcca ggtaattcca ggacttcttc ttaaagagca
     1501 ggttatgcga acactcgtgg atcagcacga acaacgaatg gttaaaaaat gctccgacaa
     1561 aataggccag caggagcacc cagtaccagg cagcatcccg aaccaaccag gccagtgcga
     1621 ccatggagcc gacacagaac aagatcgcca aaaaggtggt tgggttcttg ccgatcagct
     1681 cccgtacttc cgggtgactg gtcaatatcg ctctggtccg ctgcttatgc ggctctggct
     1741 cgttggaaaa ccgaaaatcc tgtggtgaca tatccgggaa ggaaatgcga attacttctt
     1801 ccgctttttc ttcatcatca tcatccgccc gcccttgttg ttcttgttgg tgcctgcgct
     1861 ttcacgcacc ttttccttac tgagcgcgag gatctcggcg gtgtgattca accgagccga
     1921 ctccgacatt ctgagttttc cgtctgaaag atcttccagg tggttgaaga tcgtttcatc
     1981 atccacggta tccttgttcc aggtcaggta cgacatcttc atgaagttgg ccaggtttcg
     2041 cgtaatgatc tcgcgctgtt gcccttcttc catctcaccg atcttttcga tcatctcctg
     2101 ggtcgtcttg ccgtaatact tatagcggat gtcaccggaa ggatatgcaa tccgtttggg
     2161 ctgcaaccgc atggcttctc tttcgggaac cggatacggc gcatcgacat ccagtttgaa
     2221 atccgacatc acgaaaagat ggtcccacag cttctgcttg taatcagcgg tttccttcaa
     2281 ttgaggattc aacacagcca tcgcattgac gatggaagcg gccacgcggg tacgcaggtc
     2341 gcgatccgga atctccacgc aatgttggac cattttctgg atcgcacgac cgtattcgga
     2401 aatgatgagt cgatcgcgct gcgtgttgta ctcgatcgta tccacaggcg gacgttgagt
     2461 agtccggacg gatttagcca ttatgaaagg tatgagggaa ccgggagcgt attccgcgta
     2521 ttcgcagatg cgagctgccg ggagcttttc acaaatgtaa ttgttgattc ggtatcaacc
     2581 aagaatgcgc agtttggcga attttagcag caattgctta ttcccgacgc cctggaaaaa
     2641 tacggttgcc ttgcggtcgt tttcccgacc ctcaatgagc atgacctttc cacgaccgaa
     2701 cttggggtgt tcaacctcca tgccttgctg gatctggctg gtatcatcac cttcaaaatc
     2761 ccgcacttcg gccgtattta ctttcttcag gttcgtcgga accgtgcgag gcggaggaac
     2821 cggcgcagaa ggtttgttct tcggagccgt tccataaacc ggtttcgaat ttccaccatt
     2881 ccgtaagccg gggcgctccc aacgatcttc caggaaatca tcctgataac ggggcttggg
     2941 acgaaggttc ttcacggctc cctcttcgag gaactgggga tcgatctctt caatgaaccg
     3001 gcttggctcg caggaaatca ggttacccca gcgataccgc gaattggcat acgagatcgt
     3061 cagctttttc tcggcgcgtg taaccgctac ataaaacaat cggcgttcct cttccaggtc
     3121 gtcccgggag tcgagcgaca tctgggatgg gaataggttt tcttcgagtc cgaccacata
     3181 aaccgccgga aactccaggc ctttcgcagc gtggatcgtc atgagcgata ccgaatcctc
     3241 gttcgctttc tcatcgtcgt cggcatcggt cagcagggca atgtcctgca tgaactgttc
     3301 cagtgtccgc aattcgggat gcgttcccgg tacgacttcg tctccatccg gagtgatgct
     3361 tcgccccgac tccgtaaatt cccggatacc gttcaacaat tcctggacgt tctcgtatcg
     3421 gctgaggcct tccggcgtct tgtcggcgta tagttcccgc atgagtccgg aagctgacga
     3481 gagctgactg gccagttcat aagcatccgt ggttttcaca cgggccgcgt acgatcggat
     3541 catggtcaca aattcgttga tgcggttcaa cgtaccggca ttgaatccgc agggattcgc
     3601 ggaaggttcg gcgatcagtg accagggatt ggtgtccttt tccgctgcga ccaggaggat
     3661 cttctcaatg gttgtcttgc ctatcccccg tgcgggcaca ttgatgatcc gcttcagggc
     3721 ttcttcgtcg ttgaagttga tgaccaggcg gaaatacgcc aacaggtcct tgatctcctt
     3781 gcgcttgtag aaggacaggc cgccgtagat ccggtaggga atgttccctt tccgcaacgc
     3841 ttcttccatc gcgcgcgact gggcgttggt ccggtagagt atcgcaaagt ccttgttcct
     3901 ccactgccgg ttcatcttgg tattgaagat gtcgctggcg accatgacgc cttcttcgtt
     3961 atcgctcagg gcacgcagca actggatctt ctcgccgatt tcattctccg tccaaacctc
     4021 tttccggatc tggcgctcgt tcttcgcgat gatgctgttg gcggcatgga cgatattctt
     4081 cgtcgagcgg taattctgtt cgagcttgaa gacattcaga tccgggaagt cccgctcgaa
     4141 cgacaggatg ttgttgatgt tggctccgcg gaaggcatag atcgactgcg cgtcgtcgcc
     4201 gacgacacag atgttctcga acgttttcgc cagcttcttc aggatgacgt actgggcgta
     4261 gttggtgtcc tggaactcat cgaccaggat atagcggaat ttatgctggt acttgtgcag
     4321 gacatccggg aacttctgga tgaggacgta catattgtac aacagatcgt cgaaatccat
     4381 tcccgacgat ttgaagcagc gtttcacata gagctcgaac aacagtccca tcttcggttt
     4441 cccggagctg ttatcatcgg cgatgaaatc cgtgttttga tgatactcca tccaggagta
     4501 aagattattc ttggcggccg atatgcggtt caagaccaca tcgggtttgt agaccttgtc
     4561 gtcgagacct tgttcacgca ggatgtcttt catcagactc ttggcgtcat cggtatcgta
     4621 gatggtgaaa ttcgacgggt agccgagtcg ctccgcttcg atccgcagga ttttggcgaa
     4681 cacagcgtga aaggtgccca tccataagtt ccgtgcttcc gttccggcga cctttgaaat
     4741 acgttcgcgc atttcacgcg cggccttgtt ggtgaaggtc agtgccagga tgttgaacgg
     4801 atcgacaccg gaatgtaaca ggtgggtgat ccggtacgtc aatacgcgtg tcttaccgga
     4861 acccgcaccg gcaatgatca tgaccggacc atcggtgtgg gtgaccgccg cccgttgggg
     4921 ctcattcaat tcatcgagat aatgctgcat ccgaattcag agaccggcag aaaaattcag
     4981 gccccgcccc ctggcaggga atttcgtaca tcctgtaatc cgctggagat tgaaattttt
     5041 gcaaaaatac gggaatacag ccggtctgga gcaggttctt ttcaacaatc ccatcagggg
     5101 tcaatttcgg ttattctatt gaagcagaag gggcaaaatc ttggaaatcc gggaagaaag
     5161 tcgtttcttt gccgtccttt taaacgcgta tccggagaat acgcatccgc ctgggtcggt
     5221 ggaaaggatc cgatcaacca tcaaaattaa cagaagatgc ctgcaaagat tcgtctgaca
     5281 cgtcatggaa agaagcacaa tgcttactac cacatcgtgg tcgcggacgg tcgtgctccg
     5341 agggatggta agtttatcga ggtcatcggt aactacaacc ccaacacgaa ccccgccacc
     5401 atcaatctcg acgctaccaa agcgttgaat tggctcaaca aaggagcaca accgaccgac
     5461 acctgtcgtg ctatcctttc ttacaagggt gtattgtacc gtaaccacct caacaagggt
     5521 gtcctgaaag gcgcgctcac agctgaacag gctgatgcaa aattcgagc
//
LOCUS       DAOTON010000065         5511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_12440_length_5511_cov_3.904142, whole genome shotgun sequence.
ACCESSION   DAOTON010000065 DAOTON010000000
VERSION     DAOTON010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12440_length_5511_cov_3.904142"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="P5292_02160"
     CDS             complement(<1..317)
                     /locus_tag="P5292_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRS37959.1"
                     /translation="MKNLLRSIFIAAGFLFVTTAASAQKWAYVNTQYILENIPEYKQA
                     QQQLDNLSVQWQKEIEDKYAIIDKLYKAYQAEQVLLTEEMKKRRQDEIMQKEKDVKDL
                     QKQR"
     gene            complement(380..2875)
                     /gene="bamA"
                     /locus_tag="P5292_02165"
     CDS             complement(380..2875)
                     /gene="bamA"
                     /locus_tag="P5292_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRS37960.1"
                     /translation="MIKRLLFFFAVLLSTPALAQIQIGGDSLSVDYGRPKDFEIGGVT
                     VSGTQFLDPGVLINISGLTIGDTIAVPGDDISNAIHNLWKQGLFSDIKVIATRIQGST
                     IFLELRLTERPRLSKFTFKGVSKSEADKIRESIKLERDKVITENVLNNTRNSVRAFFI
                     EKGYLDTKVDIREIRDSSYANREILEIIVDKQERIKVGRINFIGNNSLPASKLRRAMK
                     DTKEKRWYKVFTSSKYLEDKYADDKVKVVAKYNEIGYRDAKVVRDSTYRIDKKRMGIT
                     IWVDEGNKYFFRNINWVGNTKHTSQELSNILGIKRGDVYDQSVLDERLYMNQNGRDVS
                     SLYMDDGYLFFQVTPVEVAVESDSIDLEMRVYEGRQARINRVTVSGNSKTNDRVILRE
                     IRTKPGQLFSRSDIIRTQRELAQLGYFDQEKLGVNQKPNPTDGTVDIDYTVEERPSDQ
                     LELSGGYGANQLVGTLGISFNNFSARNFFKKGAWHPLPSGDGQRLSLRAQTNGKFFQS
                     YNASFTEPWLGGKKPNSFSVTAFHSIQSNGRTKEDPLRSSIKITGLSVGLGKRLRKPD
                     DYFTIYNEVNYQYYELQNYGSTFLFSDGTANNLNFRETISRNSVDAPIYPRSGSNISL
                     SLQFTPPYSLLNNVNYKTATDQEKYKFIEYHKWKFTSQWYTRLAGNLVLHARIQYGFL
                     GLYNRNLGASPFERFFLGGDGLSGFALDGREIIALRGYQNNSLTPIDAGTGRQVGGTI
                     FDKYTLEMRYPLSLNPSATIFALAFAEGGNSWLRFREFNPFEARRSLGAGIRIFLPVF
                     GLLGLDWGYGFDKLPDAPDANGPQFHFSLGQQF"
     gene            complement(2879..3619)
                     /locus_tag="P5292_02170"
     CDS             complement(2879..3619)
                     /locus_tag="P5292_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRS37961.1"
                     /translation="MSLKDKIIPEKLPLHVAVIMDGNGRWAQRQGQLRVFGHQHGVKA
                     VRDTTEAAAELGIKFLTLYAFSTENWNRPKEEVDALMELLIHTIHQEKETLNKNKIRL
                     QAIGDMDALPPKCREELNAAIRDTATNDRMTLILALSYSSKWELSRAMRLIAEDVKAG
                     RLDPASISEEHIADRLCTREFPDPELLIRTSGEHRISNFLLWQIAYAEFYFTDKLWPE
                     FGKEDFYAAIADYQSRERRFGLVSEQLK"
     gene            complement(3757..4614)
                     /locus_tag="P5292_02175"
     CDS             complement(3757..4614)
                     /locus_tag="P5292_02175"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRS37962.1"
                     /translation="MLQKKSILIIVVSVLLSGSPARAQYDEMGIVIGGSNYKGELSNH
                     LFNTDFLHFAAGGFFRHNWDRHWAWKLAINYGKVSGDDAKSNDGFQLDRNLSFHSTIL
                     EVSPQIEFNFFPYETGSYQYPVTPYVFTGFSVFKFNPKADFGDETVELQPLGTEGQGF
                     DGKKKYRRFSFALPIGGGIKISAGKIGIGIEVGARKTFTDYLDDVSTVYPDPVALRSN
                     NGNLAVALSDRSFSSRDTLMVLTGIKGKQRGNSFDKDWYLFGGITLYFRVGSILRDIC
                     EPFKRRRYF"
     gene            complement(4659..>5511)
                     /locus_tag="P5292_02180"
     CDS             complement(4659..>5511)
                     /locus_tag="P5292_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37963.1"
                     /translation="LPLASEAQRWKRQRYEFSFGAGASNFLGELGGANQIGTNYFKDL
                     EWSQTRFAVAIGLRYKLSNYFALKSHLTYGRVAGDDKLTTEYFRNYRNLNFFSDIYEF
                     NVNFEGAFQQEQVGHRYRLRKVRGLKGYELYTYGFVGVGVFYFDPRTDYLGSVVRLQP
                     LGTEGQGLIASRTKYSLIGICIPVGIGFKYTIDRYWGVGLEIGLRKTFTDYIDDVSTT
                     YFDFANYPDANDLAAVLADRSNKSDPKVTTAGQQRGDPRDKDSYMFGIFSLNYKLRTG
                     RNNLPRF"
BASE COUNT         1185 a         1362 c         1606 g         1358 t
ORIGIN      
        1 cgctgcttct ggaggtcttt cacatctttc tccttttgca tgatctcgtc ctggcggcgc
       61 tttttcatct cttccgtcag caatacctgc tcagcctggt aggctttgta aagcttgtcg
      121 atgatcgcgt acttgtcttc gatctctttc tgccattgaa cgctcaggtt gtcgagctgt
      181 tgctgcgcct gcttgtattc cgggatgttc tccaggatat actgcgtgtt gacataggcc
      241 catttctgtg cagaggctgc tgtcgttacg aacaggaatc ctgcggcgat gaaaatggaa
      301 cggaggaggt ttttcatttg tactgcgacc atgggttcgc tcctttctgt tttgaagggt
      361 tgcaaatgta ggaatgtaat cagaactgtt gtccgaggga gaagtggaat tgcgggccgt
      421 tggcgtcagg agcatccggg agtttgtcga atccgtagcc ccagtcgaga ccaagcaggc
      481 cgaagaccgg caggaagatg cggataccgg caccgagtga cctgcgtgct tcgaatggat
      541 tgaattcgcg gaaacgaagc caggaattac cgccctcggc gaacgccagc gcgaaaatgg
      601 tggcggaagg attcagcgag agcggataac gcatttcgag cgtgtatttg tcaaagattg
      661 tgccacccac ctggcgaccg gtaccggcgt cgatcggggt aagcgagttg ttctggtagc
      721 cacgcagcgc gatgatctcg cgtccgtcga gcgcgaagcc ggaaagtccg tcgccgccga
      781 ggaagaagcg ttcaaacggg gaagcgccga gattgcgatt gtagagaccc aggaagccgt
      841 actggatgcg ggcatgcaac accaggttgc cggcaaggcg ggtgtaccac tgagacgtga
      901 acttccactt gtggtactcg atgaatttgt acttctcctg gtcggtcgcc gtcttatagt
      961 tgacgttatt caacagcgag taaggaggcg tgaattgcag cgagagcgaa atgttggagc
     1021 cgctgcgcgg atagatcggc gcgtcgaccg aattgcggga gatcgtttcg cggaagttca
     1081 ggttgttggc cgtaccgtcg ctgaaaagga aggtcgatcc gtagttctgc agttcgtagt
     1141 actggtaatt gacttcgttg tagatcgtga agtaatcgtc gggtttgcgc agtcgtttgc
     1201 ccagtccgac cgacagaccg gtgatcttga tcgaggagcg cagtggatcc tctttcgtcc
     1261 tgccgttcga ctgaatggag tggaaggctg ttaccgagaa actgttcggc ttcttgccac
     1321 ccagccaggg ttcggtaaag gaagcgttgt agctctggaa gaacttaccg ttggtctgcg
     1381 cacgcaggct cagtcgctga ccgtcgcccg atggaagcgg gtgccaggcg cctttcttga
     1441 aaaagttccg ggccgagaag ttgttaaacg aaataccgag tgtaccgacc agttggttcg
     1501 cgccgtaacc gccggaaagt tcgagctggt cggacggccg ttcttccacc gtatagtcga
     1561 tatcgaccgt gccgtcggtt gggttgggct tctggtttac tcccagcttt tcctggtcga
     1621 aataaccgag ctgcgcaagt tcccgctggg tgcggatgat atcggagcgg ctgaacaact
     1681 ggcccggttt ggtgcggatc tcccgcagga tcacacggtc gttggtcttt gaattgccgc
     1741 tcaccgttac gcggttgatg cgggcctgac ggccttcgta aacgcgcatc tccagatcga
     1801 tggagtcgct ttccaccgcc acttcgaccg gtgtcacctg gaagaacagg tatccgtcgt
     1861 ccatatagag cgaactgacg tcgcggccgt tctggttcat gtacagtcgc tcgtcgagga
     1921 ctgattggtc gtagacatcg ccacgtttga tgccgagaat gttagagagt tcctgggagg
     1981 tgtgcttggt gttcccgacc cagttgatgt tccggaagaa atacttgttg ccttcgtcga
     2041 cccagatggt gatgcccatc cgcttcttgt cgatgcggta ggtggaatcc cggacgacct
     2101 tggcatcccg gtaaccgatt tcgttgtatt tggcaacgac tttcaccttg tcgtcggcgt
     2161 atttgtcttc gaggtatttc gaagaggtga agactttgta ccagcgcttc tctttggtat
     2221 ccttcatggc ccgacgcagt ttcgaagccg gcagcgaatt gttgccgatg aagttgatgc
     2281 gtccgacttt gatgcgctcc tgcttgtcca cgatgatctc caggatctcg cggttggcat
     2341 aactgctgtc gcggatctca cggatgtcga ccttcgtatc gagatatcct ttctcaatga
     2401 agaacgctcg cacggagttg cgggtattgt tgagtacgtt ttcggtgatg accttgtcgc
     2461 gctccagctt gatgctttcc cggatcttgt cggcttccga cttgctgaca cccttgaagg
     2521 tgaacttcga caggcgcggg cgttccgtga ggcggagttc caggaaaatg gtgcttccct
     2581 ggatgcgggt agcgattact ttaatatcgg agaagaggcc ctgcttccag aggttgtgga
     2641 tagcatttga aatatcgtca ccgggcacgg cgatggtatc tccgatggtc aggcccgaga
     2701 tgttgatcag tacgccaggg tccaggaact gggttccgct taccgtaacg cctccgattt
     2761 cgaagtcttt gggccggcca tagtcgacgg aaagggaatc gccgcctatc tggatttgtg
     2821 ccagcgccgg ggtgctaagc aatacggcga aaaagaaaag cagtcgcttg atcacaggtt
     2881 atttgagttg ctcgctgacg agaccgaatc gtcgttcgcg ggattggtag tcggcaatag
     2941 cggcatagaa gtcttctttg ccgaattccg gccagagttt atcggtaaag tagaattcgg
     3001 cataggcgat ctgccacaaa aggaagttgc tgatccgatg ttctccgctg gttcggatca
     3061 gaagttccgg gtccgggaat tcccgggtgc acaaacgatc ggcgatgtgt tcttcgctga
     3121 tggatgcggg atccagacga cctgctttca catcttcggc tatcaaacgc atggcgcggc
     3181 tcaattccca cttggaggaa taactcaggg cgaggatcag cgtcatgcga tcattcgtgg
     3241 cggtgtcgcg tatggcggcg ttcaattcct cccggcattt cggcgggagc gcgtccatat
     3301 caccgatcgc ttgcaaacgg atcttgttct tgttcagggt ttccttttcc tggtggatcg
     3361 tgtggatgag caattccatc aaggcgtcga cttcttcctt ggggcggttc cagttttccg
     3421 tggagaaggc gtataaggtg aggaatttga tgcccagttc agcagcggct tcggtcgtgt
     3481 cccgcacggc ttttacccca tgctgatggc cgaatactcg cagctggccc tgccgttgcg
     3541 cccaacgtcc attgccgtcc atgatgacag ccacatgcaa cggcaatttt tccggaatta
     3601 ttttgtcttt taggctcatt cctccgtgaa aaggaggcgc aatttactaa aagggaatga
     3661 aggaacttgg ttgaatttgt gtaaatgaac attcaaaatg cgccaacttc acgcaaatga
     3721 caatataaaa aagctaaaaa ctattaaact ttcggatcag aaataccgac gccgtttgaa
     3781 cggttcacag atgtcgcgca gaatgctacc gacgcgaaaa taaagggtaa tgccgccgaa
     3841 caggtaccag tctttgtcaa acgaatttcc ccgttgcttc cctttgatac cggtcaggac
     3901 catcaaggta tcgcgggaag agaatgaacg gtcggaaagt gcgaccgcga ggttgccgtt
     3961 attactgcgc agcgctaccg gatcgggata aacggtactg acatcgtcga ggtaatcggt
     4021 gaaggtcttt cgggcaccga cttctattcc gatgccgatc ttccctgcag agatcttgat
     4081 acctccgccg atcgggaggg cgaaactgaa gcgacgatat tttttctttc cgtcgaagcc
     4141 ttgtccttcg gtgccgagcg gctgcagttc aacggtctcg tcgccaaagt ccgctttcgg
     4201 gttgaacttg aaaacggaaa agcccgtgaa tacatagggc gttaccggat actgatagct
     4261 gccggtttcg taaggaaaaa aattgaactc gatctgagga cttacctcga gaatggtaga
     4321 atggaaagac aggttccggt cgagttgaaa tccgtcgttc gacttcgcgt catcgccgga
     4381 tactttaccg tagttgatgg ccagtttcca ggcccagtga cgatcccaat tgtgacggaa
     4441 aaagcccccg gcagcgaaat gcaggaagtc ggtattgaag aggtggttgg aaagctcacc
     4501 cttgtagttt gatccgccaa tgacgattcc catctcgtca tattgcgcac gggctggtga
     4561 ccctgacaac aggacggaaa caacgataat cagtatgctt tttttctgaa gcatgcccaa
     4621 ggcctccgtt aacggaaaat tcggggagaa attgaagatc agaaccgggg caggttattc
     4681 ctgccggtgc ggagcttata gttcaggctg aagatcccga acatgtaaga atccttatcg
     4741 cggggatctc cgcgttgctg gcccgctgtg gtgactttcg ggtcgctttt attggaacgg
     4801 tcagccagta cggctgccag atcgttcgca tccgggtaat tggcaaagtc gaagtaggtt
     4861 gtactgacgt cgtcaatgta gtcggtgaag gtcttccgca gtcctatttc aaggccgacg
     4921 ccccagtagc ggtcaatcgt gtatttgaat ccgataccca ccggaatgca gattccgatg
     4981 agcgaatact tggtacggga agcgatcagg ccctgtcctt cggttcccag aggctggagg
     5041 cgcacgactg aaccgaggta atcggtacgc ggatcaaagt agaagacgcc gacgccgacg
     5101 aagccgtagg tgtaaagttc atagcctttg agaccgcgaa ccttgcggag gcggtagcgg
     5161 tgacctacct gttcctgctg gaaagcgcct tcgaagttga cattgaactc atagatgtcc
     5221 gagaagaaat tcaggttccg gtagttccgg aagtattccg tggtcagttt gtcatccccg
     5281 gcaacgcgac cgtaggtcag gtggctcttg agtgcgaaat aattggatag cttgtaacga
     5341 aggccgatcg cgacggcgaa acgggtctga gaccattcga ggtctttgaa gtagttggtg
     5401 ccgatctgat tggcgccgcc caattcacct aaaaagttgc tggcccctgc cccgaagctg
     5461 aattcgtagc gctgacgctt ccaccgttgc gcctcagacg caagcggcag g
//
LOCUS       DAOTON010000066         5498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_12478_length_5498_cov_3.543083, whole genome shotgun sequence.
ACCESSION   DAOTON010000066 DAOTON010000000
VERSION     DAOTON010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12478_length_5498_cov_3.543083"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="P5292_02185"
     CDS             <1..1162
                     /locus_tag="P5292_02185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS37964.1"
                     /translation="PVHSEPIQGYRIHWADSILEAGIASEWTSFPSSSTDTLDAGYLA
                     ALEQAQPGDLSFRYGLLYYRDDGQPVARIALQVLNFTSRNFKFPKTTWKTLLAMIALR
                     FRRFRVIMAGNLFSVGKPMIDCPDENHLPALLQATEIEARRLGYDLLLLKDLPEYWHE
                     SVFENAGYHPFEADLTMQLSLPASWNSFDEYLHALTKKYRQRAVKIRRSGALLERRWL
                     TPAEVRTGANAINRLFRSVQEKQVVRMGIVDVCYFLALSTALGDRFRMLGYFLQDRLV
                     GFASYIRHGDELEVHYIGMDYTVNATHQLYFNILLDGIEHAIRDRYAKLELGRTAREA
                     KAVCGCRPHPFRDRFKTRNAGIQRLLNRMAASFTEDEGASWQIRHPFGERMI"
     regulatory      1202..1383
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1471..3651
                     /locus_tag="P5292_02190"
     CDS             1471..3651
                     /locus_tag="P5292_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS37965.1"
                     /translation="MHRKLTRTGLLLWLLHASDPVSAQAPDTLQRELKEVTITASRTP
                     EPIGAIGRSVSVLDRQAIENTSCTTLGELLSRLAGIYLLGAGQTPGSNQTIFMRGSNS
                     NQTAIYLDGVRINDVSSVNGVADLSELPLSSLDRIEILRGSHSTVFGSAAVGGVILLQ
                     SRKPEKEGWNLRFGTGAGIFRTSGSQWNTDINLGHRWKNGCWVSASTELLSVNGLDAT
                     VDTTDGTSAIPRDQDDWKKQNFSIAGGWTSGNWSSQIRYRRTGMQTDIDRSAYTDDDN
                     YNLDFTRQLISGELKWKKGNWQLGLNGGWTSSERLSLNDSSRIDLTGNYDGNFADDRY
                     TGQQLNGDLFAHYLGQSWSMLAGINASSEQMNQEHFYYSTAFGSVFESRSTLDSLNPD
                     AQLTAPYLKIRLGGDLVSHRLRPFSLVAGIRSVHHSSFGSIRTFELNPSWQLEENSLL
                     FASFSSGYNPPSLYQLYAPETYQAWDGTSYTPTTRGNKDLTPEYSQALEFGIRQQLAS
                     SEFSFSIFRNRTEDIIDYVYLWNAAVPVDSLGTDWSRDDYRGDRYINIGNQTAYGAEL
                     SVRTKLSNKATATASMSMIDGYLDIKSSDELQTNGYTLQAFSNGAFLSEGKRLRGLIR
                     RPNTMTAGIELSVRENLVLSARVQYVSARNDNYYEALYGPYGALGRRTVDDYSLLDVY
                     FHWKLSPTFQLTGRVENLLDTRYEEILGFSSRGRGAFLNLQYSL"
     gene            3706..4230
                     /locus_tag="P5292_02195"
     CDS             3706..4230
                     /locus_tag="P5292_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37966.1"
                     /translation="MNRKPTSTTETLWILGLILVAAATRIIPHPFNFTALGAMSLFSG
                     SRLQDSRKAYLLPFAALFMTDLILGFHASILPVYLGFGIYVWLGSRMRNRESVLKIGA
                     SAITGSILFFLLTNLPFWYVDIQLYPMTLEGVRQSYTAGLPYFANQLAADLLYAAALF
                     GIHSWVTRKSVSLA"
     gene            4738..>5498
                     /locus_tag="P5292_02200"
     CDS             4738..>5498
                     /locus_tag="P5292_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37967.1"
                     /translation="MDYPSKVIEMPAGGNLILGTVGSTNGAVIGSHGSSDIWLVRTDA
                     NGNMLWQSCFGGSAMDVAGSFVALPNGHLLIAGYTASQNGDVTANHGQFDAWLLEVDA
                     TGHLVWQKTYGGSASDLLYTITLAPNGDILLGGGTYSNDGDVNGQHGNQDFWLLRTDA
                     SGNLLWQRSLGGSNHEVCYGLAVSTSGHLLACGGTNSTNGQVTGKRGAYDGWVVSLDG
                     NGNLQWSACMGGTMSESFSALTLNGNELLLAGYTSS"
BASE COUNT         1294 a         1479 c         1408 g         1317 t
ORIGIN      
        1 gccggtacat tccgaaccaa ttcaaggcta ccgaattcat tgggctgaca gtatcctgga
       61 ggcgggaatc gcttccgaat ggacatcgtt cccatcgtcc tcgacagaca ccctcgatgc
      121 cggttatctc gcagccctgg aacaagcgca acccggagat ctgagtttcc ggtatggtct
      181 gctttactac cgggatgatg gtcaacctgt agcccgtatc gcccttcagg tacttaattt
      241 cacttccagg aattttaagt ttccaaaaac gacctggaaa acactactgg ccatgatcgc
      301 gctgcgtttt cgtcgcttcc gggtaatcat ggctggtaac ctgttttccg tcgggaagcc
      361 catgatcgat tgtcccgacg aaaaccatct tcccgcgcta ctgcaggcaa ccgaaatcga
      421 agcccgacgg ttgggctacg atctactctt gttgaaagac ttgccggagt attggcacga
      481 atcagtcttt gagaacgcag gctatcatcc gttcgaggcg gatctgacca tgcaactatc
      541 gctgccggct tcctggaact cctttgacga atatctgcac gcacttacaa agaaataccg
      601 ccaacgcgca gtcaagatcc gacgatccgg agccctcctg gaaaggagat ggctcacacc
      661 agcggaagtc cgaaccggcg caaacgcgat caatcgattg tttcgttcgg tccaggaaaa
      721 gcaagtggta cggatgggaa tagtggatgt atgctatttc ctggcgttat ctacggcgct
      781 tggcgatcga tttcgcatgc ttgggtattt cctccaagac cggctcgtgg ggttcgcatc
      841 gtatatccgc catggcgacg aattggaagt tcattatatc ggtatggact atacagtaaa
      901 tgccacccac caactctatt tcaacatcct gctggacggg atcgagcatg caatccgtga
      961 ccgatatgct aagcttgaac tgggtcgaac cgcccgggaa gccaaggctg tttgtggctg
     1021 tcggccgcac cccttccggg accggttcaa aacccgaaac gcagggatcc agcggctctt
     1081 gaaccggatg gccgcaagtt tcaccgaaga tgaaggagca tcgtggcaga tccgacatcc
     1141 gtttggcgaa aggatgatat aagcgtcggt taacctctgt ttttttgttt cctgaattgt
     1201 ctatcttgca ccggaatgcg gttccgcctt cggcggatga aaagggaatc acgtgaaaac
     1261 cgtgaacagt tcccgctgct gtgaatccca atagaggttt tgccttattg ccactgttcc
     1321 acgaacggga aggcggtgaa accgggatga gtcagaagac ctgccgtgtt cgttaaccat
     1381 cagctttcgg gtaaaaagca tgaatggcga gagtctcttt tctttcgttt cattccgcga
     1441 tttgcccggt gttgagtaaa gcattactct atgcaccgga aacttacaag gacaggtctg
     1501 ttgctttggc tgctgcacgc atcagacccg gtatcggcgc aggcgccgga taccctgcag
     1561 cgagaactca aagaagtcac gatcaccgcg agccgtacgc cggaacccat cggcgcgatc
     1621 gggcggagtg tatccgtact ggacagacaa gcgattgaga atacttcctg tacaacgttg
     1681 ggagagctct tgtcgcgtct ggcaggcatt tatctgctcg gagccggtca aacacccggt
     1741 tccaaccaga ccattttcat gcgtggcagc aacagcaatc agacggctat ctaccttgat
     1801 ggagtccgga taaatgatgt cagcagtgtg aacggtgttg ctgatctgag tgaattgcca
     1861 ttgagttcac tggatcgaat tgaaatattg agaggttcgc acagtacggt ttttggaagt
     1921 gcagcagtcg gaggcgtaat cctgttgcaa agccgcaagc cggaaaaaga aggctggaac
     1981 cttcgcttcg gtaccggagc gggcattttt cgcacctccg gaagccaatg gaatacggac
     2041 atcaatctgg gtcatcgctg gaagaacggt tgctgggtat ccgcttcaac agagctgttg
     2101 tcggtaaacg gcctggatgc caccgtagac actaccgatg gaacttccgc cattccccgt
     2161 gaccaggatg actggaagaa gcagaacttt tcaatcgccg gcggttggac gtcgggaaac
     2221 tggtcgtcac aaatcaggta tcggcgaacc ggcatgcaaa ccgacattga ccgaagcgcc
     2281 tataccgatg acgacaatta caaccttgat ttcacccgtc agttgatcag cggcgagctg
     2341 aagtggaaaa aaggcaattg gcagctgggg ctgaatggcg gctggacgtc cagcgagcgt
     2401 ctctcgctga atgactcgtc ccggatcgac ctaaccggta actatgatgg caattttgcc
     2461 gatgaccgct ataccggcca acaactgaac ggagatctgt tcgcccacta tctcggtcaa
     2521 tcgtggagca tgctcgcggg gatcaacgcc tccagcgagc aaatgaatca ggaacatttt
     2581 tactacagca cggcgttcgg ttcggttttc gaatcccgct ccacactgga ttccctgaac
     2641 cccgatgccc aactcaccgc accttatctg aaaatcaggt tgggtggtga tttggtttca
     2701 cacaggttgc gtccattttc attggtcgcc ggcattcgct ccgtacacca cagcagcttc
     2761 ggatcgatcc ggacctttga gctcaatcca agctggcaat tggaggagaa cagtttgttg
     2821 tttgcatcct tttcgtctgg atataatccc ccctcccttt accagctgta cgcaccggaa
     2881 acctatcagg cctgggacgg caccagctac acgccgacca cacgcgggaa caaggacctt
     2941 acccccgaat attctcaagc gctcgaattc gggatccgtc agcaattggc gagttcggag
     3001 ttcagctttt ccattttccg gaaccgaacg gaagatatca tcgattacgt ttacctgtgg
     3061 aacgctgcag ttccggtgga ttcgctcggg accgattggt cacgggacga ttaccggggt
     3121 gaccgataca tcaacatcgg caaccaaacg gcctatggtg ctgaactatc cgtacgaacg
     3181 aaactcagca acaaggcgac agcgactgca tccatgagca tgatcgacgg ttacctggac
     3241 atcaaatcgt cggacgaact gcaaaccaac ggctatacgt tacaggcctt cagcaatggt
     3301 gccttcctat cggaaggcaa gcggttgcgg ggtttgatca gacgccctaa tacgatgaca
     3361 gccggaatcg aattgagcgt acgggaaaat cttgtgttga gcgcacgcgt tcagtatgtc
     3421 agcgcccgca acgacaacta ctacgaagca ctctatggtc cctacggtgc cttaggacgt
     3481 cgtaccgttg acgactattc actgctcgac gtatatttcc attggaaact ctctccaact
     3541 ttccagctaa ccggcagggt cgaaaaccta ctggataccc gatacgagga gattcttggt
     3601 ttttcgagcc ggggacgcgg ggctttcctg aatcttcaat attccctctg aaatcaagac
     3661 ctctcctaca aaaccaaaaa aactatcttt gtacagcatc ttactatgaa ccgtaagccg
     3721 acctccacta cagaaaccct ctggatactc gggttgattc tggttgccgc cgcgacccgc
     3781 attattccgc atcccttcaa ctttacggca cttggtgcca tgtccctgtt ttcaggatcc
     3841 aggcttcagg acagccggaa agcctacttg cttccgtttg ccgccctgtt catgactgac
     3901 ctgattttgg gatttcatgc ttccattctc cccgtctacc tgggttttgg aatttacgtt
     3961 tggttgggta gccgaatgcg taaccgggaa agtgtactga agattggtgc ctcggccatt
     4021 accggatcga tcctgttttt cctgctgacc aacctcccat tctggtatgt tgacattcaa
     4081 ctctatccga tgacgctgga aggggttcga caaagttata ccgccggact accttatttc
     4141 gccaatcaac tggctgccga cttgctttat gctgccgcct tgttcggcat ccattcctgg
     4201 gtgacccgga agtcggtttc cctggcctaa aaacttgatt taaaaaaaga aacccagcct
     4261 tacggggctg ggttttctta tttcagcctt acggggctga ttggaaccat tcaatcttac
     4321 gggattgaaa aaaggcttta ctgatggttc agagggggtg ttcctgatcg aacacagtgg
     4381 gtttaacgcc ccgtctaatc caaaggattg gtcatcggga acctatccct tccaattgta
     4441 agaatttcgc gtcttgccaa tattttcact aaaaatcgac cctttcatcg gcagctcaac
     4501 ccaaacaatc gcttacccta aagcctcatt agcccataat tcacagcctt ttaccgaata
     4561 aaattgaaag cccgcggtgt cggggtggat atttacatca ctccaccgac ctgaagtcac
     4621 cgcatgagat atatttcgat tttcttcctt tttctggccg ttttttcgaa aaacgagaaa
     4681 attcaggcat tagctccccc ggttagcgga attaactgga gctctggagg aagcggaatg
     4741 gattacccgt caaaagtgat cgaaatgccc gcaggtggaa atctgatcct ggggaccgta
     4801 ggctcgacca atggcgctgt gatcggttct catggatcct cggacatctg gctggtcaga
     4861 acggatgcaa acggcaatat gctctggcaa tcctgcttcg gtgggagcgc catggacgtt
     4921 gccggaagtt tcgtagcact cccgaatgga cacctgctga tcgcgggtta taccgcctca
     4981 caaaatggtg atgtaaccgc caaccacggt caattcgatg catggctgct cgaggttgac
     5041 gcaacgggcc acctggtctg gcaaaaaacc tacggcggct ccgcttccga tctgttgtac
     5101 accattaccc tggctcccaa tggagacatc ctgttgggtg gcggaaccta ttccaatgat
     5161 ggggatgtga atggtcagca tggcaaccag gacttctggc tgctccgtac ggatgccagc
     5221 gggaacctgc tctggcagcg aagccttggc ggcagtaacc acgaagtatg ctacggactt
     5281 gccgtctcca ccagcggtca tcttcttgct tgcggcggta ccaactccac caacgggcag
     5341 gtgaccggca agcgcggcgc ttatgatggc tgggtggttt ccctcgacgg taacggcaac
     5401 ttgcaatggt ctgcctgcat gggtggaacc atgagtgaaa gcttcagtgc cctgacactc
     5461 aacgggaacg aactccttct cgctggttac accagttc
//
LOCUS       DAOTON010000067         5434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_12653_length_5434_cov_3.408440, whole genome shotgun sequence.
ACCESSION   DAOTON010000067 DAOTON010000000
VERSION     DAOTON010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12653_length_5434_cov_3.408440"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..612
                     /locus_tag="P5292_02205"
     CDS             190..612
                     /locus_tag="P5292_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37968.1"
                     /translation="MKKLLIIGGLLGVLTAGGIGVYLFNKPHQSIKSVDAEFQSTAPE
                     LVTQFQDNEEAANKQYLGKVVEVNGVVSDIATDEKGVLNITLQGGDLSGVTCQFEKAN
                     QESALTLKKGQSVSIKGICTGILMDVVLVDCVVSSNPS"
     gene            673..975
                     /locus_tag="P5292_02210"
     CDS             673..975
                     /locus_tag="P5292_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37969.1"
                     /translation="MPVLRISPSGKILYANKAATGLLKEWNCMASEKLPDQLTSEYPL
                     LLNLWSDEELAIPVSGGTCRCAVIGFPDADYIGIYVHHLTAYTDPTAVDERIMASV"
     gene            1021..1587
                     /locus_tag="P5292_02215"
     CDS             1021..1587
                     /locus_tag="P5292_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRS37970.1"
                     /translation="MKTSILIAFLAFLTAGVSAQNRYFTKTGKIYFDATTPSSPEKIE
                     ATNEKATSVIDAGSGAMEFAVLLKAFAFERALMQEHFNENYVEADKYPKSTFKGSVTN
                     IKEVNLSKDGSYPVKLKGQMTLHGVTKEVEADGSLTVKGGAIVSGKSNFKILFSDFNI
                     VIPNLVKDKVAKEATIKVDVNYEPLKAS"
     gene            1745..2641
                     /locus_tag="P5292_02220"
     CDS             1745..2641
                     /locus_tag="P5292_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRS37971.1"
                     /translation="MKKNYSLLLLGLLLTTLTVRAQDDMLSLLGNDAPVREPVKNAFK
                     SSRVINGHSMEMIAGGVLDFRILHRFGRVNQGVKELFGLDQASMRMGFDYGITDHLTA
                     GVGRSTFKKELDGFLKFRLLQQSTGKGAFPFSIILLSGMTLDGSEWTQPERNNYFTSR
                     LAYYHQVILGRKFNDVFSLQLTPTLLHRNLVPTTEAPSDLYALGVGTRVKLNKRIALT
                     ADYSYNFNPGPSPAYRNPLSIGFDIETGGHVFQLHFSNTRGMNERAYLADTEGKWGKG
                     DIMFGFNLSRVFTLDSRGRQGK"
     gene            complement(2629..3057)
                     /locus_tag="P5292_02225"
     CDS             complement(2629..3057)
                     /locus_tag="P5292_02225"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HRS37972.1"
                     /translation="MFTPEVQIAMAFLLLRGVAGALFFFQGYDKLFNIGVPGVATAFT
                     DANRKDILPTNLFRFFITISSWVEFICGALLFFGLFRGFALTCLSLNLLFVAIAFSNF
                     KAMWDMQYFFPRMVFVTALLLMPWAADRWSLDLLLEAYLP"
     gene            3139..3882
                     /locus_tag="P5292_02230"
     CDS             3139..3882
                     /locus_tag="P5292_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRS37973.1"
                     /translation="MFNLIAFLLLAFLSEIVGTIGGFGSSVFFVPIAQFLFDFQTVLM
                     LTAILHDFSNTAKLFLFYRHLDFRLLLLYGIPSTVFVILGAWLSKDLYLQYLELILGV
                     FLLLFGSFVMMYPSFRLSQDRVSAVTGGSVAGFLAGLIGTGGAIRGMSLAAFDLEKST
                     FIATSAGIDFFVDLSRTIVYWQNGFYRSEFSWYIPLLILIAFAGSYAGKRLLGKIAQP
                     TFRKIVLSLLILIGITMIVKTLTGNGLAV"
     gene            complement(3856..4554)
                     /locus_tag="P5292_02235"
     CDS             complement(3856..4554)
                     /locus_tag="P5292_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRS37974.1"
                     /translation="MFLYSALIVGFVGSLHCVGMCGPIALSLPLQDKASWRFLFGRLM
                     YNSGRVVTYTFLGVLAGLIGHTIAMAGFQRYLSIASGLLILLAVAVPVIARYLERLSA
                     SSAKWTGVIRSGFRKLFSSHSGTRLFAIGLVNGLLPCGMVYLAVAAAVATGDVVSSAT
                     YMFLFGLGTVPAMFAMTVAGSFFGFRFQKAVRQLTPVIAVVIGVLLIIRGLTTNPKDC
                     CRNHKASYSQSITR"
     gene            complement(4557..5000)
                     /locus_tag="P5292_02240"
     CDS             complement(4557..5000)
                     /locus_tag="P5292_02240"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRS37975.1"
                     /translation="MRFNWGFGIVVAAGLFVAFILNLVYQCSQQQVDLVTDKYYEKEL
                     KYQEQIDQEKNTLALSGKLTVVAGPGEVVIRYPEDHQSGQMTGEVRFFRPDNAKLDFT
                     VPVNSGSANEQRIATEKVKQGWWNVQVQWNHAGKPFYQQEKVLIN"
     gene            complement(5005..>5434)
                     /locus_tag="P5292_02245"
     CDS             complement(5005..>5434)
                     /locus_tag="P5292_02245"
                     /inference="COORDINATES: protein motif:HMM:NF023045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HRS37976.1"
                     /translation="RFRFTARIIGYIAVLLVLLTTLITLLVLRSDVETTVLRTPGQMY
                     QQLTDGTISNLYNIQIVNKTFNDLPLDVDLVSPAGEIRYVGQELNMVRQDSLAEGVFF
                     IVLDRNQIHQIKTPVVISVKSAGKEIDRVETNFMGPNTKQ"
BASE COUNT         1353 a         1513 c         1371 g         1197 t
ORIGIN      
        1 gggatcaccc tggaaggcgc aaccaatctc aagacctatg ccgataacgg ccgactgatg
       61 gggtccatcg aacaccgctc cggctacagt gccatgccgc aaagcgcacc aaaactatct
      121 gactgcgaaa tccggacgat ccaggcatgg atcagtgccg gtcaaccata gtacttaatg
      181 agaacgaaaa tgaaaaagct tctgatcatc ggtggattgt tgggcgtatt gacagccgga
      241 ggtataggcg tgtacctctt caacaaacct caccagagca tcaaatccgt tgatgcagag
      301 tttcagtcaa ccgcgcccga actcgtaacg caatttcagg ataacgagga agctgccaac
      361 aagcaatacc tgggcaaagt cgttgaagtg aatggagttg tcagcgatat cgctaccgac
      421 gagaaaggtg tactgaacat caccctgcag ggtggtgacc tgtccggcgt gacgtgtcag
      481 ttcgaaaaag cgaaccagga gagcgcgctt acgctgaaga aaggccagtc cgtatccatc
      541 aaagggatct gcaccggcat cctcatggat gtagtactgg tcgattgtgt cgtatcctcc
      601 aaccccagct aagccatgaa gagtctcgat caacccgttg agttccgatc tgtagcattt
      661 cgtgaaatgt caatgcctgt tctccgcatc agtccgtccg gcaagatcct gtacgcgaat
      721 aaggccgcga ccggtctgct gaaggaatgg aattgcatgg ccagcgaaaa gctcccggat
      781 cagctgacct ccgagtatcc gctgttactg aacctgtggt cggatgagga attggctatc
      841 cccgtttcag gcggaacttg tcgttgcgcc gtgatcgggt ttcccgacgc cgactacatt
      901 ggcatctacg ttcaccacct gacagcctac acggatccaa ccgccgttga cgaacgcatc
      961 atggccagtg tctgatctgg caccattcgt gcattaacaa tcaaaaccca ataaaatcca
     1021 atgaaaacat ccatcctgat cgcgtttctg gctttcctga ctgcaggcgt aagcgcccag
     1081 aaccgctact ttaccaaaac cggcaagatt tactttgacg ctaccacccc aagtagcccg
     1141 gaaaaaatcg aagccaccaa cgaaaaagca accagtgtga tcgatgccgg ttccggcgcg
     1201 atggaattcg cggtcctgtt gaaagcgttt gcgttcgagc gcgcgttgat gcaggagcac
     1261 ttcaacgaaa actatgtgga ggcggataag tatcccaagt ccacattcaa aggaagcgtt
     1321 accaacatca aagaggtcaa cctttccaaa gacggaagct acccggtaaa actgaaaggc
     1381 cagatgacgc ttcacggcgt aacaaaagaa gtcgaagccg acggctcgtt gacggttaaa
     1441 ggtggcgcga tcgtcagcgg taaatccaac ttcaagatcc tgttcagcga tttcaatatt
     1501 gtcatcccga acctggtcaa agacaaagtc gccaaagaag ctacgatcaa agtggatgtg
     1561 aattacgagc cgctcaaggc ttcttaagga atacggtttc ctccccacca cggccctgta
     1621 aggccagtca caaccggcag catcatcgat ctgccgctcg ttccaacaaa ttccgatctg
     1681 gtacctcacg ggccggtgaa ccaacaactc accggcccgt gttgtatcct attgccgctg
     1741 caccatgaaa aaaaactact ccctcctgct attgggtctc ctgctgacca ccttaacggt
     1801 acgtgcccag gacgacatgt tgtccctgtt gggaaatgac gcgcccgttc gcgaacctgt
     1861 aaagaacgcc ttcaaatcca gccgggtgat caatgggcat tcgatggaaa tgattgccgg
     1921 cggcgtactg gatttccgca tcctgcaccg cttcggtcgt gtcaaccagg gagtcaaaga
     1981 actcttcggg ctcgatcagg ccagcatgcg catgggcttt gactacggca tcacggatca
     2041 tttgaccgca ggcgtaggcc gcagcacgtt taaaaaagag ctcgacggtt ttctgaagtt
     2101 ccgcctgctg cagcaatcca ccggcaaggg agcgtttcca ttttccatca tcctgctatc
     2161 gggcatgacc cttgatggta gcgagtggac acaaccggag cgaaacaact acttcacatc
     2221 aaggctggcc tattatcatc aagtcatcct tggtagaaaa ttcaacgacg tcttcagtct
     2281 gcagctaacg ccgacactac ttcaccgaaa tctcgtgcct accaccgaag ccccgagtga
     2341 tctgtacgcg ctgggcgtgg gtacacgtgt gaaattgaac aagcgcattg cgctgacagc
     2401 cgattactct tacaacttca atcccgggcc ttcaccagct tatcgcaatc cactctcgat
     2461 cggattcgat atcgaaaccg gtggccatgt atttcaattg catttttcca atacacgcgg
     2521 aatgaacgag cgggcttacc tggccgatac cgaaggaaaa tggggaaaag gcgacatcat
     2581 gttcggcttc aacctgtcgc gggtctttac actcgacagc cggggtcgtc aaggcaaata
     2641 ggcttcgagc aggaggtcca ggctccagcg atccgcggcc cacggcatca gcaagagcgc
     2701 ggtaacgaac accatccggg gaaagaaata ctgcatatcc cacatcgcct tgaagttcga
     2761 aaaggcgatg gcaacgaaca gcaggttgag tgacagacag gttaaagcga atccgcggaa
     2821 caagccgaag aacaacaagg caccgcagat gaattccacc catgaactga tggtaatgaa
     2881 aaagcgaaag aggttggtgg gcaggatatc cttcctattg gcatcggtga aggccgtagc
     2941 cacgcccggc accccaatat tgaagagctt atcatatccc tgaaagaaga ataaagcgcc
     3001 tgctactccc cggagcaaca ggaaggccat cgcgatctgc acttctggtg taaacatgtt
     3061 cccgactttt ctcaaattta cgaaattgcc ggcaatcggg cggtcatcga atgatcgttc
     3121 accaacctca ccaccgggat gttcaacctg atcgcattct tgctgctcgc cttcctgtcg
     3181 gaaattgtcg gaaccattgg cggtttcgga tcgtccgtct ttttcgttcc aatcgcccag
     3241 tttttgttcg actttcagac ggtcctgatg ttgacagcca ttctgcacga tttcagcaat
     3301 accgcaaagc tcttcttgtt ttaccggcac ttggacttcc ggctattgct gctctacggc
     3361 attcccagca cggtattcgt cattcttggc gcctggttga gcaaggatct ttacctccaa
     3421 tatctggaat tgatcctggg cgtgtttctg ctgctgttcg ggagttttgt catgatgtac
     3481 ccgagcttcc ggttgtcaca agaccgggtc agcgcggtca cgggcggatc ggttgccgga
     3541 ttcctcgcgg gattgatcgg gaccggtggc gccattcgtg gtatgagcct ggccgccttc
     3601 gatctggaga agtccacttt catcgccaca tccgcaggca tcgacttctt tgtcgacctc
     3661 agccgcacta tcgtctactg gcaaaacggt ttttaccgat cggaattcag ttggtacatt
     3721 cccctcttga tcctgatcgc ctttgcaggc tcttatgcgg ggaaacgact gttggggaaa
     3781 atagctcagc ccacttttcg caagatcgta ctcagtctgc tgatcctgat cggcattacc
     3841 atgatcgtaa aaaccctaac gggtaatgga ctggctgtat gaagccttgt ggttccggca
     3901 acaatccttg gggttggtcg tcagtccccg gatgatcagg agcacgccta tcactacggc
     3961 gataaccggc gtcaattggc gaaccgcctt ttgaaaccgg aagccgaaaa aactgccggc
     4021 aaccgtcatg gcaaacatgg ccggtaccgt acccaatccg aacaggaaca tataagtcgc
     4081 actggacacg acatctcccg tcgcgaccgc ggctgcaacg gccaggtaca ccatcccaca
     4141 cggtaacaat ccatttacca agccgatggc aaagagacgt gtgccggaat gcgaagaaaa
     4201 aagtttccgg aaaccggaac gaatgacgcc ggtccatttt gccgaggagg ctgaaagccg
     4261 ttccaggtag cgcgcgatca ccggtaccgc taccgcgagc aggatcaaca gtccggaagc
     4321 gatcgacaag taacgttgaa atcccgccat cgcgatggta tgcccgatca gtcctgccag
     4381 taccccaagg aacgtatagg taacgaccct cccactgttg tacatcaatc gtccgaagag
     4441 gaaccgccag ctcgccttgt cctgtaatgg aaggctgagc gcgatcggtc cgcacatgcc
     4501 aacacaatgc aggctgccga caaaaccgac gatgagtgcg gaatacagga acatcttcag
     4561 ttgatgagga ctttctcctg ctgatagaaa ggcttacccg cgtgattcca ctgtacctgg
     4621 acgttccacc agccctgctt gactttctcc gttgcgatcc gctgctcgtt ggctgatccg
     4681 gagttcaccg gaacggtaaa atcaagtttc gcattgtccg gacgaaagaa gcgcacctcg
     4741 ccggtcattt gtcctgattg atggtcttcc gggtaacgga tcacgacttc acccggcccg
     4801 gctaccacgg taagcttacc cgagagcgcg agggtgtttt tctcctgatc gatctgctcc
     4861 tgatacttga gctccttttc atagtactta tccgtcacca ggtcgacctg ctgttgagag
     4921 cattggtaga ccaggttgag aatgaacgcg acgaacaaac cggccgcgac gacaattccg
     4981 aatccccagt tgaagcgcat gttgctattg tttagtattg ggtcccatga aattcgtttc
     5041 cacacgatcg atctccttgc ccgcagactt aacgctgatc acgaccggcg ttttgatctg
     5101 gtggatctgg ttacgatcca ggacgataaa gaaaacacct tccgccaatg aatcctgccg
     5161 gaccatgttc agttcctggc ccacatacct gatctcaccg gctggcgata ccaagtcgac
     5221 atccaacgga agatcgttaa acgtcttgtt gacgatctga atgttgtaga ggttggagat
     5281 cgtaccgtca gttagctgct ggtacatctg tccaggtgtc cgcagcacgg tcgtttccac
     5341 atcggagcgg agcaccagca aggtgatcag cgtggtcagc agcaccaaca agaccgcgat
     5401 gtagccgatg atcctggccg taaaccggaa tcgc
//
LOCUS       DAOTON010000068         5421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_12686_length_5421_cov_3.456206, whole genome shotgun sequence.
ACCESSION   DAOTON010000068 DAOTON010000000
VERSION     DAOTON010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12686_length_5421_cov_3.456206"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..892)
                     /locus_tag="P5292_02250"
     CDS             complement(200..892)
                     /locus_tag="P5292_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRS37977.1"
                     /translation="MQRTIPLHEIAFLDIETAAVYPSFESMPEAWQQLWRRKSVGFRD
                     PEPPEDSYRKAGIYAEFGKVICIVVAIPDRFGAIQYEVFDDPDEATLLRSFGLFLHTA
                     FRSGRKWLCAHNGKEFDFPFIARRMLVNGLPLPDALDTAGRKPWEVQHLDTMELWKFG
                     DYKAFTSLTLLAQLFNLPSPKDDLDGSMVGETYWVEQDLPRIVRYCRKDVETLVQVYL
                     RMRGNMVGATLL"
     gene            complement(1212..1628)
                     /locus_tag="P5292_02255"
     CDS             complement(1212..1628)
                     /locus_tag="P5292_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37978.1"
                     /translation="MIDMRLNFYLLVFLLIHSVLDVYGQHIGDVYVYDSTLSRFSDYD
                     FDDQGNLLFSGYVGPRNATGTDPFGNLSFDGYWYENGLTCKADSTGFLQWMTVSGNNN
                     AQENVQEIRFDNVNRAKEAFKSSMPPDDWSRLRADG"
     gene            complement(1857..2264)
                     /locus_tag="P5292_02260"
     CDS             complement(1857..2264)
                     /locus_tag="P5292_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37979.1"
                     /translation="MLKDIHRPEVKNIVLAAVKEPNEESGTMEWHVYLVNLNDEVIRG
                     VLVSSKGYGELDGKEVKTSILRHLIGNVDPQDYARIEMIMDNLFGLSNEYWVSFYMGS
                     DMYDKKYIFLPESIKEENFTNIPVLNKRGVMLR"
     gene            complement(2484..>2699)
                     /locus_tag="P5292_02265"
     CDS             complement(2484..>2699)
                     /locus_tag="P5292_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRS37980.1"
                     /translation="VTDYYGYHWWLATVDGHRVVYARGILGQYIIVIPDMQLVVVRLG
                     IERGTKEEDQHYSDFKAYVRGAVEMVR"
     assembly_gap    2700..2709
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2710..3680)
                     /locus_tag="P5292_02270"
     CDS             complement(<2710..3680)
                     /locus_tag="P5292_02270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRS37981.1"
                     /translation="MANARFRRILFRTLGLIFLFLLSGSVALMITGYSYVFKTLVYTY
                     PGIDDLELFSTRIIDDPAPVRWPYASDYQDRRPSAPLSGELEKNKSVAYLVVRNDSLL
                     YEQYWDRYDTVSPSNSFSVAKSIVGLLVGIAADEHLLELDQPIGRYLPHFNTEAGNTC
                     SIRELLMMSSGLNWDESYASLFSYTTEAYYGTDLPALTNKLKVIEEPGSVFRYMSGNT
                     LLLGMILERATGERLSDYAAKKLWKPLGATAPAFWSLDHADGIEKAYCCFYSNARDFA
                     RIGQLVLDSGMSQGRQIVSKDYIRQMTTPIGLPDERGAPLGRHRRGRL"
     gene            complement(3734..3952)
                     /locus_tag="P5292_02275"
     CDS             complement(3734..3952)
                     /locus_tag="P5292_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37982.1"
                     /translation="MNRSIVELRQLDERQLPDDDHPDRGRDISPAEFDTWMDAIISKT
                     KNFEPKAPCDIGQATIDALRSLKPTTEG"
     gene            complement(4008..4724)
                     /gene="lipB"
                     /locus_tag="P5292_02280"
     CDS             complement(4008..4724)
                     /gene="lipB"
                     /locus_tag="P5292_02280"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015329756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRS37983.1"
                     /translation="MPRVLFSELGRFGYQEAWDLQERLFQELVDRKLRNRNLRQEEQE
                     PLHHYLLFCEHPHVFTLGRSGDAGHLLADEQTLKEIGAIFIKNNRGGDITYHGPGQLV
                     GYPILDLDDFFTDIHRYLRYLEEAIIRVLGSYGVKAGRYPGYTGVWIDPEDDAKARKI
                     CAMGVRCSRWVTMHGFAFNVNTDLSYFEHIVPCGIKDKDVTSLQRELGRAIDMSEVSH
                     RLKHELAALFGMQLEPAELG"
     gene            4965..>5421
                     /locus_tag="P5292_02285"
     CDS             4965..>5421
                     /locus_tag="P5292_02285"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRS37984.1"
                     /translation="MRTSLCLLLLFLLLPLFLSAQSHSVSGKLSGSDGSALIGASVIL
                     QRLPDSSFVAGTVTDATGNFSLLAPRPGDYVIKLAFVGFEDLFIRRNITTNIELGTLK
                     LRDKATVLSTVNIVELTPPALQKGDTTEYNSKAFKTQPDANAEDLVTKMP"
BASE COUNT         1325 a         1485 c         1322 g         1279 t
ORIGIN      
        1 catgatggca gaacagatgc gtaaatagat ctgcttcttg cgacgcaatt tcttcgggct
       61 gaattgacga tgcaatccgg ccatgaccgg atcacgttca aatacttgat ctatggaagg
      121 agtcatcacg ggcggcatcg cgatcatgcg cgactttttc aagatacccg tttcgtatca
      181 agtacacaac caattgccat tacagcaggg ttgctcctac catatttcca cgcattcgca
      241 ggtaaacctg cacgagcgtt tcgacgtcct ttcggcaata gcgtactatt cgtggaaggt
      301 cctgttccac ccaataggtc tcaccgacca tgctgccgtc cagatcgtcc ttcggactgg
      361 gcaggttaaa caattgagcc agcagggtaa gcgaagtaaa cgctttgtaa tcgccgaatt
      421 tccaaagctc catggtatcc aggtgctgaa cctcccaggg ttttcgacct gccgtatcaa
      481 gggcatcggg tagtggcaat ccatttacca gcatccttct ggcaatgaac ggaaagtcga
      541 actctttccc gttgtgcgcg cacaaccact ttcgtccgct tcgaaatgcg gtgtgcagaa
      601 acaatccgaa acttcttaag agtgtcgcct catcgggatc gtcgaatact tcgtactgga
      661 tggcaccgaa acgatccggt atggccacca cgatgcagat tactttccca aattcggcgt
      721 aaatgcctgc cttgcgatag ctatcctccg gaggttccgg atcgcgaaag ccgaccgact
      781 ttcgcctcca caactgctgc caggcttccg gcatcgactc gaaggaagga taaacagccg
      841 ccgtctcaat atccagaaac gcgatctcat gcaggggaat agtgcgttgc atgccacaag
      901 actacgcgga tgcgcggtcg ctggcaacca atctatgctg tttcttttag gaggggcgag
      961 ggacgaggga cgggggacgg gggacgaggg acggggggcg agggacgagg gacgagggac
     1021 gagggacgag ggacggggga cgaaaaaaat gacgcgttta taagaaacgg gataattcca
     1081 ggacgcgatg gatggtataa accttggcta ttgctttatc atgcgtaagg ttgccaggcg
     1141 attcccggat gttacttgta tcagataaac ttcgtttgga atgtggctgc agtcgagcgt
     1201 ttctgagata gctagccgtc cgctctgagc cttgaccagt cgtccggtgg catcgaagat
     1261 ttgaatgctt ccttcgcccg gttcacatta tcgaatcgaa tctcctgcac attctcctgg
     1321 gcattgttat tccccgaaac ggtcatccac tgaagaaacc ccgtggaatc ggctttgcag
     1381 gtcagtccgt tttcatacca atatccgtca aaactcaggt tcccaaacgg atccgtaccg
     1441 gtagcgtttc ttggacccac ataacccgag aagagcagat tgccctggtc atcgaaatca
     1501 taatcgctga agcgcgagag ggtgctatca taaacataca catcaccgat gtgttgtccg
     1561 tacacatcta atacggaatg gatcagcagg aaaacaagta agtagaaatt aagtcgcatg
     1621 tcgatcattt tcggaaagaa atgaaaaaac cactaaagtg ccatgcggtc tccaacctat
     1681 tccggtactg tcatccaagc aaaggcgaac gcaacaattc aggaggtaaa aggaagaaaa
     1741 gacgtacttg gtgggcttcg cttcggattc atgatccgtc attcatccca aggtgcggca
     1801 agaaaccggc attccagtca ccaaattcca atcactaata acaaatctcc aacctctcac
     1861 ctcaacatca ctccccgctt attcagcacc gggatgttgg tgaaattttc ttccttgatt
     1921 gactccggta aaaagatgta cttcttgtcg tacatatcgg aacccatata gaaactgacc
     1981 caatactcgt tactcaatcc gaacaggttg tccatgatca tctcgatgcg cgcataatcc
     2041 tgcggatcca cattcccgat aaggtggcga aggatggatg ttttcacttc tttcccgtcg
     2101 agttcgccat accctttcga gctcaccaat actccgcgga tcacttcatc gttcaggttg
     2161 acgaggtaca catgccattc catggtaccg gattcttcgt tcggctcctt cacagcagca
     2221 aggacgatat tcttgacttc aggccggtga atgtctttca gcataggagg cgtaaaggta
     2281 cggaaaagtt ggggcggggt ggattggata ttggaggttg gttattcgtg atttaatatc
     2341 ggtgattggc ggtgggtcgg gttgaaagat cattccaaac tctacaagca ataaaactgg
     2401 aaacgcggaa ctcgaaaccc gcctgcctgc cgcagcttta gcgtaggcag ggaacccgga
     2461 accagttcgt cacacctccc tccttaccgg accatctcga cagctccccg cacgtaggcc
     2521 ttgaagtcgc tgtaatgctg gtcttcctct ttggtgccgc gttcaatgcc gagccgcacc
     2581 acgacgagtt gcatatccgg gatcacgatg atgtattgac caagaatccc tcttgcatac
     2641 acgacccgat gaccatccac ggttgcaagc caccagtgat aaccataata atcagtgacn
     2701 nnnnnnnnna gccggcctcg ccgatgtcgg cccagtggcg ctccccgctc atccggcaag
     2761 ccgatgggcg tggtcatttg tcggatataa tctttcgaca ctatttgcct gccttgagac
     2821 attccactgt caagcaccaa ctgtccgatc cgggcaaaat cgcgggcatt ggaatagaaa
     2881 caacaatacg ccttttcaat tccgtcggcg tgatcgagcg accagaacgc gggagcggtt
     2941 gcccccagcg gcttccacag ttttttcgca gcgtaatcag acaaacgttc gcccgtggct
     3001 cgttcaagga tcattcccaa caacaacgtg ttgccgctca tgtaccggaa cacggaaccg
     3061 ggctcctcga tcaccttgag cttattcgtc agtgccggta agtcggttcc ataatacgcc
     3121 tcggtagtat aggagaagag cgaagcatac gattcatccc agttcaggcc ggagctcatc
     3181 atcaacaact cccggatgct gcaggtgttc cccgcttccg tattgaaatg cggcagatag
     3241 cgcccgatcg gctggtcgag ttccaggagg tgttcatcag ccgcgatccc cacgagcaac
     3301 ccgacaatgc tctttgcaac ggaaaaagaa ttgctgggcg ataccgtatc gtagcggtcc
     3361 cagtattgtt cgtacaacag gctgtcgttg cgaactacca ggtaggcaac gcttttgttc
     3421 ttctccaact ccccgctgag cggtgcggag ggccggcggt cctgatagtc ggatgcatag
     3481 ggccaacgaa cgggtgccgg atcgtcgatg atgcgggtgg aaaacagctc cagatcatcg
     3541 atacccggat aggtatagac cagtgttttg aaaacgtagc tgtaaccggt aatcatcagg
     3601 gccaccgagc cactgaggag aaacaagaag atcagcccga gtgttcgaaa caggatccga
     3661 cggaagcgtg cgttagccat gctctcaagt taggcatcgc actgccgtgt cagtaatcgt
     3721 ccggaagagt ttttcagcct tccgttgttg gcttcagact gcgtaacgca tcgatcgtcg
     3781 cctgcccgat atcacagggg gccttcggtt caaaattctt cgtcttggaa atgatcgcat
     3841 ccatccaggt atcgaactct gcaggcgaaa tatcccgccc acgatccgga tggtcatcgt
     3901 cgggtaactg gcgttcatcc aattggcgca actctacaat ggatctgttc atgttttttg
     3961 aaggtacgaa aaatttgtcg gaattccaaa tgcgagaata gggcaattca ccccagctcc
     4021 gccggttcca actgcatacc gaaaagcgcg gccagctcgt gtttcaatct gtgactcact
     4081 tctgacatat cgattgcccg gcccaattcc cgttgcagcg aggtcacatc cttgtctttg
     4141 attccgcagg gtacgatgtg ttcgaaatag gaaaggtccg tattgacatt gaacgcgaaa
     4201 ccgtgcatgg taacccagcg ggagcaacgc acacccatcg cgcagatctt acgggccttc
     4261 gcatcatctt ccgggtcgat ccagacacct gtatagccgg gataacgacc agctttcaca
     4321 ccataggaac cgagcacgcg gatgatggcc tcttccaaat accggaggta gcggtgtata
     4381 tcggtgaaaa aatcgtcgag gtcgaggatc ggatagccta ccagctgccc gggaccgtga
     4441 tacgtgatat cgccgccccg gttattctta atgaaaatcg ctccaatttc ctttaacgtt
     4501 tgttcatccg ccaataaatg accggcatcc ccgcttctac ccagggtgaa tacgtggggg
     4561 tgctcgcaga aaaggagata atgatggagt ggctcctgtt cttcctgccg aagatttcgg
     4621 ttccgaagtt tgcgatctac caattcctgg aacagccgtt cctgtaaatc ccaggcctcc
     4681 tggtatccga aacgccccaa ctcgctgaac aatactcgcg gcatcgcgca aagttaaatg
     4741 tttcgagtct tcctcctcct ttactccagg ccgaagcgat tcttcgaatt tcaaacttta
     4801 acaaatccga ttcgaaagcc tttgaatctc cgataacatt tctctttccc ttctcctctg
     4861 gaatcaatcg atccgcctac aaatgcaagt ctccgcttcc gattccctac ttttgcacgg
     4921 tcgcgatttg acccaaccgt tcgcttcgcg acatcatacg aactatgcgt acttccctct
     4981 gcctgctcct gctgtttcta cttttaccac ttttcctttc agcccaatct cattccgtca
     5041 gcggcaagct gtccggatcc gatggcagcg cgttgattgg cgcctccgtc attctgcaac
     5101 gacttcccga ctcctccttc gtagcaggaa cggttacgga tgcaaccggc aatttctcgc
     5161 tcctggcacc ccggcctgga gactatgtta tcaaactcgc gtttgtcggc ttcgaagatc
     5221 ttttcattcg acgaaacatc actacgaaca tcgaattagg gaccctgaag ctgcgcgaca
     5281 aagccacggt cttatcgact gtcaatattg tagaactcac gcctcccgcg ctccagaaag
     5341 gcgacaccac cgaatacaac agtaaagcgt tcaagacaca gcccgatgcc aatgccgagg
     5401 acctggtaac aaaaatgccg g
//
LOCUS       DAOTON010000069         5311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_12983_length_5311_cov_3.964992, whole genome shotgun sequence.
ACCESSION   DAOTON010000069 DAOTON010000000
VERSION     DAOTON010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12983_length_5311_cov_3.964992"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="P5292_02290"
     CDS             <1..553
                     /locus_tag="P5292_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37985.1"
                     /translation="GFRQSNAMTDIPTALVNRDFTHREYYGADLQLSLDWKAGITVLR
                     GEYIQGDQPGLGSDTKSPNTNSPVLKPIYNRAFNGAYFYLCQGIGHTPFSAVIKYDWY
                     DPNTDVKGDEIGKATANGLKATNATDLRFDTWGFGAAWRMDANTRISAYFDLVKNETS
                     KNLNLYTVDQHDNVFTLRVQTRF"
     gene            593..1411
                     /locus_tag="P5292_02295"
     CDS             593..1411
                     /locus_tag="P5292_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRS37986.1"
                     /translation="MKKTLIILSILATAFQALGQKIVVKGSDTVLPLTQKEAERYMKK
                     MSGNSVTVVGGGSGVGIAALMDGTTDIAMSSRRLKMDERLKLQEAGKPFKEVVVANDA
                     LSVIVHPANKVTRLTREQIEGIFTGKIKNWKEVGGADLTIVAYSRETSSGTYEFFKEH
                     VLNRKNYASTVLNMPATGAIIQSVSQTKGAIGYVGLAYVTKEVKDVAVSYDGGKNYVL
                     ATKENAINKTYPVVRPLYYYYQTSKEKQVKPFVDFILSSEGQQIVDEVGYIRLK"
     gene            1439..2644
                     /gene="pstC"
                     /locus_tag="P5292_02300"
     CDS             1439..2644
                     /gene="pstC"
                     /locus_tag="P5292_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRS37987.1"
                     /translation="MNTRFKRFLESLVEGLLTISGTVTTFTVLLIVLFLFREGASLFS
                     TSPVENNNLLVVHAGNPVSHLSATQCKDIFDQKITHWNEIGGKPDSIRIVTINELTNY
                     FSEEELGAEFEFLPDRINQLVDSFPDILAVINAKYRNASFPGKVIEIDKNNLPDFLGG
                     KEWFPTAEPVVQLGVLPLILGTLMVSIFAILFALPIGLATAIYLAEVADERVRKVLKP
                     VIELLAGIPSVVYGFFGLIVIVPMIHDVFQLPVGETALAGSIVLAIMALPTIITVSED
                     AIRNTPRAMKEASLALGANRWQTIHRVVIPYSISGISAAAILGIGRAIGETMAVLMVT
                     GNAAVIPHSLLQPVRTIPATIAAELGEAPQGGVHYKALFALGCILFLLTMLINLWVES
                     ISRKQKRHR"
     gene            2647..3501
                     /gene="pstA"
                     /locus_tag="P5292_02305"
     CDS             2647..3501
                     /gene="pstA"
                     /locus_tag="P5292_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRS37988.1"
                     /translation="MTKQLKEKLAFWTFRILSLFVVGVLFWILYFIFIRGWKTISWEF
                     LTSMPEDGMTAGGIYPAIIGTLCLMAGSMLFSFPIGIMAGIYLNEYMKDSWFKRFVKM
                     MTNNLAGVPSIVFGLFGMSLFVNTLEFGDSILAGSLTLSLLALPVVIRVTEEALLAVD
                     QSFRQGSLALGATKLQTIRRVVLPIATPNIITGLILSVGRVSGETAPILFTVAAYFLP
                     KLPSSIFDQVMALPYHLYVISTSGTDIQASRDMAYGTALVLVMIVLLLNLAANALRRY
                     FGKKVKMN"
     gene            3508..4266
                     /gene="pstB"
                     /locus_tag="P5292_02310"
     CDS             3508..4266
                     /gene="pstB"
                     /locus_tag="P5292_02310"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRS37989.1"
                     /translation="MNKIETRNVHLYYGDFHALKGIDIAIEANTVTALIGPSGCGKST
                     YLRLFNRMNDLIDNVRIEGDVLLDGRDVYKKDIGMRMDELRKRVGMVFQKPNPFPKSI
                     FENVAYGLRVNGIGNESFIEERVETSLRQAALWEEVKDKLKKSAFELSGGQQQRLCIA
                     RALAIEPSVLLMDEPASALDPISTAKIEELIYELKSKYTIIIVTHNMQQAGRVSDKTA
                     FFYLGELIEYDTTPTIFTNPRDKRTQDYVTGRFG"
     gene            4325..5002
                     /gene="phoU"
                     /locus_tag="P5292_02315"
     CDS             4325..5002
                     /gene="phoU"
                     /locus_tag="P5292_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRS37990.1"
                     /translation="MMTHLDVELKRLKDESVELFELVISQLRKGRIALLDFDKDLARE
                     VNFNEKRVNALELKIDKDCENIFALFNPVAIDLRFVLAVLKINSNLERIGDIADGIAR
                     YVIEADAPFHGDLLKVTRLDEMYTLAIEMLEDVRKCFNEEDTRLARGVFKKDDVLDDI
                     NGSANRVVEALIRKEAEHIAPALHIISTIRKLERTGDQVKNIAEEIIFYLEAKVLKHG
                     SGKVIED"
     gene            complement(5015..5245)
                     /locus_tag="P5292_02320"
                     /pseudo
     CDS             complement(5015..5245)
                     /locus_tag="P5292_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666793.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
BASE COUNT         1301 a         1490 c         1334 g         1186 t
ORIGIN      
        1 aggtttccgt cagtccaacg ccatgaccga catcccaacc gcgttggtca accgggactt
       61 cacccaccgc gaatactacg gagccgatct tcaactcagc ctcgactgga aagccggcat
      121 cacggtactt cgtggcgaat acattcaggg cgaccaaccc ggactcggtt ccgatacgaa
      181 gagtcccaat accaacagcc ctgttctcaa accgatctac aaccgcgcat ttaatggcgc
      241 ttacttctat ctctgccagg ggatcggcca tactcccttc tccgcagtca tcaaatatga
      301 ctggtacgat ccgaatacgg acgtgaaagg tgatgaaatc ggaaaagcta cggcaaatgg
      361 tttaaaagcc accaatgcaa cggacctccg gttcgacacc tggggattcg gagcagcctg
      421 gcgtatggat gccaacaccc gcatcagcgc ctatttcgac ctggtcaaaa atgaaaccag
      481 caagaacctt aacctctaca cggtcgatca gcacgacaac gtgttcacgc ttcgtgtaca
      541 gacacgattc taacattcga taaccgagat acaactatca gcagataaga aaatgaaaaa
      601 gacactgatt atcctgtcga tcctggctac cgcctttcag gccctgggtc agaaaattgt
      661 cgtaaaaggc agcgacactg tgcttccgct tacacaaaaa gaagcggaac ggtacatgaa
      721 aaagatgtcc ggaaattcag tcacggtcgt cggtggcgga tcgggcgttg gaattgccgc
      781 cctaatggat ggtactacgg atatcgccat gtcatcccga cgactgaaga tggacgagcg
      841 tctcaaactt caggaggccg ggaagccgtt taaggaagtg gtagttgcca atgacgcgct
      901 ttccgttatc gtacatcctg ccaataaagt aaccaggcta acccgcgaac agatcgaagg
      961 gatcttcacc ggtaagatca agaactggaa agaagtcggc ggagcggatc tcacgatcgt
     1021 ggcttactcc cgcgaaacca gttcgggcac gtatgagttc ttcaaagagc atgtgttgaa
     1081 tcggaagaac tatgcttcca ccgttctgaa tatgccggcg accggcgcca tcatccagtc
     1141 cgtcagtcag accaagggtg cgatcgggta tgtcggcctg gcttacgtaa cgaaagaagt
     1201 gaaagacgtt gcagtttcct acgatggcgg caagaactat gtcctggcca ccaaggagaa
     1261 cgccatcaac aagacctatc cggtcgttcg tccgctctat tattactatc agacttccaa
     1321 agaaaagcaa gtgaaaccgt tcgtggactt catcttgtcc tccgaaggtc agcagatcgt
     1381 agacgaagtc ggttacatcc ggcttaaata aacctgcgac aaccctcaga aaaaggttgt
     1441 gaatacacga tttaaacgat tcctggaaag cctcgtcgaa ggcctcctga cgatcagcgg
     1501 taccgtaacc acgtttacgg tgctgctgat cgttcttttc ctgttccggg aaggcgcttc
     1561 actcttttcc acctcaccgg tcgagaacaa taacctcctg gtcgtgcatg cgggtaatcc
     1621 cgtcagccac ctcagcgcga cgcagtgcaa agacattttt gatcagaaga tcacccattg
     1681 gaatgaaatc ggtgggaagc cggattcgat ccgcatcgtg acgatcaatg aactgaccaa
     1741 ttatttttcc gaggaagaac tgggcgccga attcgagttc ctaccggacc ggatcaatca
     1801 attggtcgac agttttcccg acatcctggc ggtaatcaat gccaagtaca ggaacgcctc
     1861 ctttcccgga aaggtcattg aaattgacaa gaacaatctt cccgattttc ttggcggcaa
     1921 agagtggttt cctaccgccg agccggtcgt acagcttgga gtgctcccac tcattctggg
     1981 caccctgatg gtctccatct tcgccatcct cttcgcgctt ccaatcggtc tggctacggc
     2041 catctatctg gcagaagtcg ccgacgagcg tgtacgcaaa gtcctgaaac cggtcatcga
     2101 actgctggca ggcatcccgt ctgtcgtata tggattcttc ggcttgatcg tcatcgtgcc
     2161 catgattcac gatgtgttcc agttacccgt cggcgaaacc gcgctggcgg gatcgatcgt
     2221 actcgccatc atggcgcttc ccaccatcat tacggtatcg gaagacgcga tccgcaatac
     2281 gccgcgtgcc atgaaggaag cctctctggc actgggcgcg aaccgctggc agaccattca
     2341 ccgggtcgtg atcccctatt ccatttccgg catctctgcc gcggccattc tgggtatcgg
     2401 gcgtgccatc ggtgagacca tggccgtgct gatggttacc ggcaacgcgg ccgtcatacc
     2461 ccattccctg ctccaaccgg tacgaaccat accagcaacg atcgcagcag aactcggaga
     2521 agcgccgcaa ggcggtgtcc actataaagc gctgttcgca cttggttgca tactctttct
     2581 tctcaccatg ctgatcaatc tgtgggtaga atccatttcc cgaaaacaga aacgccatcg
     2641 ttgagtatga ccaaacaact taaagaaaaa ctggccttct ggacgttcag gatcttaagc
     2701 ctgttcgtgg tcggggtgct cttctggata ctctacttca tcttcatcag aggctggaag
     2761 acgatatcct gggagttcct taccagcatg ccggaagatg gcatgaccgc cggtgggatt
     2821 tatccggcca tcatcggaac gctttgcctc atggccggca gcatgctctt ttcgttcccc
     2881 atcggtatca tggccggcat ctatctgaat gaatacatga aggattcctg gttcaagcga
     2941 tttgtcaaga tgatgaccaa caacctggcc ggtgtacctt cgatcgtctt cggactcttc
     3001 ggcatgtcgc ttttcgtgaa tacactggag ttcggcgatt ccatcctggc cggatccctc
     3061 accctcagcc tgctggcatt gcctgtcgtg atccgggtaa cggaggaagc cttgctggcg
     3121 gtcgatcaat cgttccggca gggaagcctg gcacttggcg cgaccaaact gcaaaccatc
     3181 cgccgggtgg tacttccgat cgccacaccg aatatcatta ccggtcttat cctttccgtc
     3241 ggcagggttt cgggcgaaac ggctccgatc ctttttaccg tagcagccta cttcctgcct
     3301 aaattgcctt ccagcatctt tgaccaggtc atggcgctac cctatcactt gtacgtcatc
     3361 tccaccagcg gaaccgatat ccaggcatcg cgcgacatgg cttacggcac ggctctcgtg
     3421 ctggtcatga tcgttctcct gctgaacctg gccgccaatg cgctccgtcg ttactttggc
     3481 aaaaaagtca aaatgaattg atgctccatg aataagatcg aaacccggaa cgtccacctc
     3541 tactacggag atttccacgc cctgaagggt attgacatcg ccatagaagc caatacggtg
     3601 acggcgctca tcggtccttc cggctgcggg aagtccacgt atctgcgact cttcaaccgc
     3661 atgaatgacc tcatcgataa tgtccggata gaaggagatg tattgctcga tgggcgcgac
     3721 gtttacaaga aggacatcgg catgcggatg gatgaactgc gcaagcgggt cggtatggtt
     3781 ttccaaaagc cgaacccctt cccgaagtcc atctttgaaa atgtcgccta cggtttgcgt
     3841 gtcaacggta tcggaaacga atcattcatc gaagaacgcg tggaaacttc tctgcgacag
     3901 gcggcactct gggaagaagt caaggacaag ttgaaaaaat ccgccttcga gctctccgga
     3961 ggacaacagc agcgactctg catcgcgcgc gcgctggcga tcgaaccttc cgtcttattg
     4021 atggacgaac ctgcatccgc gctcgatccg atctcaacgg ccaagatcga ggaactcatc
     4081 tacgaactca agagcaagta caccatcatc attgtcaccc acaacatgca acaggccggt
     4141 cgtgtttcgg ataagacggc cttcttctat cttggggaac tgatcgaata cgacaccaca
     4201 ccgaccatct ttaccaatcc ccgggacaaa cgcacccagg attacgtaac cggccgattc
     4261 ggctgagcgc tgttggctca tagtagccgt acgattatca ttaaaaactg tatcaacatc
     4321 cttcatcatg acgcacctgg acgtcgaact caaacgactc aaagacgaat ccgtcgaatt
     4381 gttcgaactg gtcatttccc agctccgaaa aggcaggatc gcactgctgg atttcgacaa
     4441 ggacctggcg cgcgaagtca atttcaacga aaagcgcgtc aatgcgctgg agttgaaaat
     4501 cgacaaggac tgcgagaaca tctttgcctt gttcaacccc gttgcgatcg acctgcgctt
     4561 tgtgctggcg gtgttgaaga tcaattccaa tcttgaacgg atcggagaca tcgccgacgg
     4621 catcgcacgc tatgtgatcg aagccgatgc accgtttcac ggcgacttac tgaaagtgac
     4681 ccgtctggac gagatgtaca ccctcgccat tgaaatgctg gaggatgtac ggaaatgctt
     4741 caatgaagaa gacacccggc tggcccgagg cgttttcaag aaggacgatg tgctggacga
     4801 tatcaacggg agcgccaacc gtgtcgtgga agccctgatc cgcaaggaag cggaacacat
     4861 tgctcccgcc cttcacatca tctccactat tcgaaagctc gaacgtacgg gggaccaggt
     4921 caagaacatt gctgaggaga tcatcttcta cctcgaagcc aaggtgctga aacacggcag
     4981 cggtaaggta atcgaagact gaggcccaaa ccgctcatcg tacgaggctg cgcagaatcc
     5041 gcttcacgag tccgggccct tcgtagatga agccagtgta cagttgcacc agatcggctc
     5101 ctgcgtccag cttttcctga gcttcttccg gtgaatgaat tccaccgacg ccgatgatgg
     5161 cgaagcgctt ttgcgcgcgc ccggcgatgt ggcggataac tgcggttgat ttacgggtta
     5221 gggggcgtcc gcttagtcca ccggtacaat ggttacatca gggtggttat tattcataat
     5281 acttaaacag gtgctgcaga ctctgcacgg c
//
LOCUS       DAOTON010000070         5301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13010_length_5301_cov_3.353603, whole genome shotgun sequence.
ACCESSION   DAOTON010000070 DAOTON010000000
VERSION     DAOTON010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13010_length_5301_cov_3.353603"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..665)
                     /locus_tag="P5292_02325"
     CDS             complement(126..665)
                     /locus_tag="P5292_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS37991.1"
                     /translation="MVIKDRTNVLLTERLELRPLTAQDAGFLFDLYTDPEVIRYTGET
                     RLRDEAHAREFLDRCTAMPASHPPVYLISIVGEEKKSGWCGLRYDDEDQVFDLGFRLE
                     RTSWGKGYATEAARAVMEEAFGQYGLIRIRARCARENIGASHVLLKLGFKAVRSFTAH
                     GYLCDEYALERHELPIGRN"
     gene            complement(659..1390)
                     /locus_tag="P5292_02330"
     CDS             complement(659..1390)
                     /locus_tag="P5292_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS37992.1"
                     /translation="MSLLMLQAPTHTLAQSERKLVREGNTAYEAKKFDQAEIQYRKSL
                     EKNKTSLPAQYNLGNALFRQDKLDEAKSQYEQAVNLKDAPAADRAKAYHNLGNSLLKQ
                     EKYQESIDAYKQSLRINPADNDTRYNLAYAQAKLQQQQNQQNQNNKQDDKQEKDQQKN
                     QNQQQQQNKDQQDKNKQQQDQQQQDQQQDQAKKDQQKPDQQKPERISKEDAEKILQAL
                     NNDEKKTQKKLNKKEAVRVRIDKQW"
     gene            complement(1429..2487)
                     /locus_tag="P5292_02335"
     CDS             complement(1429..2487)
                     /locus_tag="P5292_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRS37993.1"
                     /translation="MFRWAHPEHLYALAVVPVLLMFYLFARDARGKRLYRFGERKLVE
                     RLLPFASVNKPWLRMFLVLFALLSLVLAWANPQFGSRLVEVKREGVDVIVALDVSNSM
                     KAEDIRPNRLERSKQLISRLIDRLENDRIGLVVFAGQAYVQLPITTDYAAAKLFLNSI
                     DTDIIPTQGTAIGSALDLCQESFVGNDNKHKVIVLITDGENHEDDPITAAKKAGEAGV
                     IVHAIGMGSPEGVPVPVYRNGSQVDYLRDRDGNTVLTKLDEMTLQQIAAETKGEYIRA
                     TNSDDGLTTVINAIGRMEKKQFGTKQYSDYEDRFQYLLAFSLLLLVLESLFSDRRSEV
                     LHRLDIFGENKNKEERSS"
     gene            complement(2489..3484)
                     /locus_tag="P5292_02340"
     CDS             complement(2489..3484)
                     /locus_tag="P5292_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRS37994.1"
                     /translation="MIPGWKFLHPQWLWLLLVIPAVLAFHFFYLRKRQPTLRFSSLTG
                     FSNVPNSCRTKLKDLPVILRSLVIAALIVALARPQSSSSSQNITTEGIDIVMALDISA
                     SMLAEDLKPNRIEAAKKVARDFIDQRPNDRIGLVVFSGESFTQCPMTTDHAVLKNLLL
                     GIQSGMLADGTALGEGLATAVNRIRNSTAKSKVIILLTDGVNNIGAIAPETAGDIAQA
                     FGIRVYTIGVGTEGMAPYPVQTPLGTQYQYMPVQIDEAVLQKIAGSTGGKYFRATSSG
                     QLKTIYQDIDKLEKTRIDVTEFRHRSEEYYPIALVAIGLLCLEYFLRQTLFRTLP"
     gene            complement(3481..4461)
                     /locus_tag="P5292_02345"
     CDS             complement(3481..4461)
                     /locus_tag="P5292_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS37995.1"
                     /translation="MSRSILPTLLIVCLLWLAMTRPVAAQIASHAEARIDTNTIRIGE
                     QIRLDLSLSGPSNARVQFPVIGDTLPGLEIVKRDSSDTLRSPDGRTITYSQRFYLTRF
                     DTGYVVLEPFLFVVADPTTGAIDSLTTEAQLISVTTLAVDTTQGIKDIKPILDVPFTW
                     QDALPYVAIGLAALLIGYGIYRYLKQRKKQPQPEKRIIPPAIPAHVRAFEELERIAAE
                     KLWQQGKIKEYHTRVTDVLRNYLEERFGIPAPEMTTDELIDRMRRKIRDIRSQELLRS
                     MLVLADLVKFAKAHPLPDEHEQLLRECREFIELNRPVVAEDMTDKQEVLP"
     gene            complement(4464..5300)
                     /locus_tag="P5292_02350"
     CDS             complement(4464..5300)
                     /locus_tag="P5292_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRS37996.1"
                     /translation="MEIKTRGLSAHIFSGEYHSAFKGRGMAFSEVREYTPGDDIRSID
                     WNVTARFGHPYVKVFEEERELTVILLVDVSASGDFGTKQQFKRQLMTELCAVLAFSAI
                     QNNDKTGVIFFSDRIEKFIPPKKGKTHILRIIRELIEFKPVNRKTDLPVALQYLTNAI
                     KKRSICFLISDFYTENFERELKLANKKHDLVALEIIDPREESLPDIGLVRFEDAETGT
                     SQWIDTGDPAVREAFQKNMHVRSRRNADLFARSGVDTVRLSTDRSYVQPLMQLFKKRG
                     SK"
BASE COUNT         1056 a         1404 c         1485 g         1356 t
ORIGIN      
        1 cggcaaccga attggaactg agactggccg tgaatacatc cgccgtagcc cgggaatgca
       61 cgggcaggag caaggtggcc agcaacatcg ccagccagct ccggagtcca aggacccgcg
      121 ttagatcaat tccgaccgat cgggagttca tgacgttcta aggcgtattc atcgcaaagg
      181 taaccgtgcg ccgtaaacga ccgcacggcc ttgaaaccga gtttcagcag tacatgggaa
      241 gcgccgatgt tctcgcgcgc gcagcgtgcc cgtatcctga tcaaaccgta ttgtccgaac
      301 gcttcctcca tgacggctcg tgcagcctct gtggcgtacc cttttcccca ggaggttcgc
      361 tccaggcgaa atcccaggtc gaacacctga tcctcgtcgt catagcgaag gccgcaccag
      421 ccgcttttct tttcttcgcc gacaatactg atcagataaa ccggcggatg ggaagccggc
      481 atcgcagtgc agcgatccaa aaattcgcgc gcatgcgctt catcccgcaa ccgggtttct
      541 ccggtatacc ggatcacttc cggatccgtg tacaggtcga acagaaagcc ggcatcctgt
      601 gcggttaacg gacgaagctc cagacgttcc gtcaacaata cgttcgttct gtctttgatc
      661 accattgctt atcgatccga acccgaaccg cctccttttt gttcaacttt ttttgcgttt
      721 tcttttcgtc gttgttcagg gcttgcagaa ttttttccgc gtcttccttg ctgatccgtt
      781 caggtttttg ctgatccggt ttttgctgat cttttttcgc ctgatcctgc tgctgatcct
      841 gttgctgctg gtcctgctgc tgcttgttct tatcctgctg gtctttattc tgttgttgct
      901 gctggttctg gttcttctgc tggtcctttt cttgcttgtc gtcttgcttg ttgttttgat
      961 tctgttggtt ctgttgttgc tgtagcttgg cctgtgcgta agcaaggttg taacgcgtgt
     1021 cgttatcggc gggatttatg cgcagcgatt gtttgtacgc gtcgatgctt tcctggtatt
     1081 tttcctgttt caaaaggctg ttgcccaggt tgtgatacgc cttggcacga tccgccgccg
     1141 gtgcatcttt caggttgacg gcctgttcgt actggctctt cgcttcgtcg agtttatcct
     1201 gccggaacag tgcgtttccg aggttgtatt gtgccgggag ggaagtcttg ttcttctcca
     1261 gggatttccg gtattgtatt tccgcctggt caaacttctt cgcttcgtaa gcagtgttcc
     1321 cttcacgaac aagttttcgt tctgattgag ccagggtatg ggtaggagcc tggagcatta
     1381 acaggctcat ggcaagcagt tgatatctca gagtgatgga agctatcatc atgatgatcg
     1441 ttcctccttg tttttatttt ctccaaaaat atccagccgg tgcagcactt cgcttctgcg
     1501 gtcgctgaag agcgattcca atacaaggag cagcagcgag aaggcaagca gatactgaaa
     1561 ccggtcttcg taatcggaat attgcttggt gccgaattgt ttcttttcca tccggccgat
     1621 cgcgttgatc acggtcgtca agccgtcgtc gctgtttgtg gcccggatgt attccccttt
     1681 cgtctctgcg gcgatttgtt gaagggtcat ttcatccagc ttggtcaaga cggtatttcc
     1741 gtcacggtca cgcaggtagt cgacctggct gccgtttcga tagaccggta ccggtacgcc
     1801 ctccggagaa cccatgccga tggcgtggac gatgactccc gcttctccgg cttttttcgc
     1861 agcggttatc gggtcgtctt cgtggttctc gccatccgta atcaggacga tgactttgtg
     1921 cttgttgtca ttgcctacga atgattcctg gcaaagatca agtgctgaac cgatggccgt
     1981 accctgggtc ggaatgatat cggtgtcaat cgagttgagg aacaacttag ccgccgcata
     2041 atcggtcgtg atcggcaact gcacataggc ttgtcctgca aagaccacca atccgatgcg
     2101 atcgttttcc aggcgatcga tcaaacggct gatgagctgt ttggagcgtt ccagacggtt
     2161 cggccgtatg tcttccgcct tcatggagtt ggatacgtcg agcgcgacga tcacgtcaac
     2221 cccttcgcgt ttcacttcta ccagtcggga accgaattgc ggattcgccc aggccaggac
     2281 aagcgacaac agcgcgaaga gtacgaggaa catccgcaac cacggcttgt ttacggaagc
     2341 gaacggtagg agacgttcca cgagtttgcg ctcgccaaag cggtaaagac gtttcccgcg
     2401 agcgtcccgt gcgaataaat agaacatcag caggaccggt acgacggcca gtgcatagag
     2461 atgttcagga tgtgcccagc gaaacatatc aggggagggt gcggaacaag gtctgacgta
     2521 agaagtactc gaggcagagc aacccgatcg cgaccagcgc gatcgggtaa tactcttccg
     2581 agcgatgacg gaactcggtc acgtcgatcc gggttttttc cagtttgtcg atatcctggt
     2641 agatggtctt caactggccg gaagaagtag cgcggaagta ttttcctccg gtcgagccgg
     2701 cgatcttttg taagacggct tcgtcgatct gtaccggcat atactggtac tgggtaccca
     2761 gcggggtctg taccggatag ggcgccatgc cttccgttcc cacgccgatc gtatagaccc
     2821 ggataccaaa agcctgtgca atgtcgccgg cggtctccgg cgcgatcgct ccgatgttgt
     2881 ttacgccgtc ggtcagcagg atgatgacct tgcttttcgc cgtgctgttg cgtatgcggt
     2941 tcaccgcggt tgccagtcct tcacccaggg cggttccgtc cgccagcatt ccgctttgaa
     3001 tacccagcaa caggttcttc agcacggcat ggtcggtcgt catcggacac tgggtgaagc
     3061 tttctccgct gaacacgacg agaccgatgc ggtcattggg tcgctggtcg atgaaatcac
     3121 gggcaacttt cttagcagct tcgatgcggt tgggtttcag gtcttcggcg agcatggatg
     3181 cggagatgtc cagcgccatg acgatgtcaa taccttcggt cgtaatgttc tgactgctgg
     3241 aactggattg cggccgggcc aacgcgacga tcaacgcggc gatcaccagg cttctcagga
     3301 tcaccggaag gtctttcagt ttggttctac agctgttcgg aacgttcgag aagcccgtca
     3361 ggctggagaa tcgaagggtg ggttgccgct tccgcaggta gaagaaatga aacgccaaaa
     3421 cggccggaat gaccaataac agccacagcc attgcgggtg aagaaacttc cagcccggga
     3481 tcatggcagc acctcctgct tatcggtcat atcttccgcc accacaggcc ggttaagttc
     3541 gatgaactcg cggcattcgc gcagtaattg ttcgtgttcg tcgggtagcg gatgcgcttt
     3601 ggcgaacttg accaggtcgg ccaataccaa catggaacgg agcaattcct ggctccggat
     3661 atcgcggatc tttctgcgca tgcgatcgat caattcatcg gtcgtcatct caggagcggg
     3721 tatcccgaag cgttcttcca ggtaattgcg cagcacatcg gtcacccggg tatgatactc
     3781 cttgatctta ccctgttgcc agagtttttc ggcggcgatc cgttccagtt cttcaaatgc
     3841 ccggacgtga gccggaatcg ccggcgggat gatgcgcttt tcgggttgcg gttgtttctt
     3901 tcgttgcttc aggtatcgat agatcccgta cccgatcagc agagcggcga gaccaatggc
     3961 gacatacggt aaggcatcct gccaggtaaa cggaacatcg aggatcggtt tgatgtcctt
     4021 gatgccttgc gtcgtatcaa ccgcgagtgt cgtgacgctg atcagttgcg cttccgtggt
     4081 caggctgtcg atcgcgccgg ttgtcggatc ggcgacgaca aagagaaagg gctccagtac
     4141 gacataaccc gtatcaaagc gtgtgaggta aaagcgctga gaataagtga tcgtcctgcc
     4201 gtcaggtgag cgcagggtgt cggatgaatc acgtttcacg atttccagcc caggcaaggt
     4261 gtcaccgata acaggaaact gaacacgggc gttggacggg ccggaaagag ataggtcgag
     4321 tcgaatttgt tccccgattc gaatggtgtt cgtatcgatc cgggcttccg catgggaagc
     4381 tatttgcgca gccacaggcc gtgtcatggc cagccagagc agacacacga tcaagagtgt
     4441 agggagaatc gacctgctca tgctcacttg cttccgcgct ttttaaacaa ttgcatgagt
     4501 ggttgaacat aggagcgatc ggtgctcaat ctgactgtat cgacgccgga acgtgcaaac
     4561 aggtccgcat ttctgcgtga tcgtacgtgc atgttcttct ggaaagcttc ccgcacggca
     4621 gggtccccgg tatcgatcca ttgggacgta cccgtttccg cgtcttcaaa tctgaccagg
     4681 ccgatatcgg gcaacgattc ctcccgcggg tcgatgatct caagcgcgac caggtcgtgt
     4741 ttcttgttcg cgagtttgag ttcacgttcg aaattctccg tgtagaaatc ggagatcagg
     4801 aaacagatac tgcgtttctt gatcgcattc gtgaggtact gcaaagcgac tggcaggtcg
     4861 gttttgcggt taacgggttt gaattcgatc agttcccgga tgatcctcag aatgtgggtc
     4921 tttcctttct ttggcgggat gaacttctcg atacggtcgc tgaaaaagat gacgccggtc
     4981 ttgtcgttgt tctgtatggc ggagaaggcg agcaccgcac aaagctccgt catcaattgg
     5041 cgtttgaact gttgcttcgt gccgaagtct ccggaggcgc tgacatcgac cagcaggatg
     5101 acggtcagtt cccgctcttc ctcgaatacc ttgacatacg gatgtccgaa gcgggcggtg
     5161 acgttccagt cgatggaccg gatgtcgtct cccggtgtat attcccgcac ctcgctgaat
     5221 gccatgccac gccctttgaa ggcgctgtgg tattcgccgg aaaagatgtg cgccgacaag
     5281 ccgcgagtct tgatctcgat c
//
LOCUS       DAOTON010000071         5286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13053_length_5286_cov_3.416937, whole genome shotgun sequence.
ACCESSION   DAOTON010000071 DAOTON010000000
VERSION     DAOTON010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13053_length_5286_cov_3.416937"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4240
                     /locus_tag="P5292_02355"
     CDS             <1..4240
                     /locus_tag="P5292_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HRS37997.1"
                     /translation="DDVTFLTSPFYQDGVIAKAVDSVAAMGVTYFTSAGNFGNKSYEG
                     AFSPMVAPLGIAGQAHDFGGNDNLLSVSLPPGNYTIVMQWQDSIYSLGQTSTGTVNDF
                     DIYLTNQYGTQYFGFNRNNLGGDPLEVLPFIVPGTSNVQANISVIRAAGNQAARFKLI
                     VYRGEIIFNEHTTGSSTITGQGNANGAITLGASNYFNTPPFGVSPPLVQNFSSRGGTL
                     VDGAIRNKPDLVAPNGGNTTVNLGAPNVDNDPFPNFFGTSAAAPHAAAVGALLRQGKQ
                     KFYGTDLSPSEVRNLLQTTALDMDAPGFDFASGAGYIQAYAALKTFAAPTPDLDSLVV
                     PSGITPGTSTFNTSVYGQFFTTQSVVMFNGSVLPTSFVNGNVLSATVPPFFGNYPLSV
                     YTPPIVPNINDGGYSDTLYFFSPVKKQVVITADDKTKKYGEQLPEFTATITVDGVPLA
                     SSGFTLTDLGLDQLTFTSSATSASNTGLFFIQPSNVFTDPGYQTLFNYSFVNGLLTID
                     PMPIVITPRDTTLTYGDKIEGFQFNYDYDDTNIDPSLRANFLGDIKQTHNSQLSNVLA
                     LVDGRVVTNGRVVTNIDLQNLAMVASGRAVTNGRVVTNGRVVTNGIEYDTTIVVDVAP
                     ASIFSYVDSMATYTGDTLYATLYSPRAGVNGRVVTNGRVVTNGRVVTNGRVVTNGRVV
                     TNGYPVLNSSNVNLGSNDNMAIIIDSTDIYAAPPGDSLYDLKSINMVTGTTVGTWSIV
                     PGAFVSSNFSISYGLGELTIEPATLTVKAEDKSATYGESPAFTYTVSGYQYQDADSPV
                     FAGPPVFEVVDAQNAVVNPPGAGSFGVRIQSMPLLQPSNYITQFEQGDLDVAKADLHI
                     TADDKSRTYGAANPALTLSYAGFVYNDGPAALTAPTASTSAEATSPAGNYPITLSGGQ
                     ADNYNLLLHDGNLRIDKANLEARADNKTMVGGTSVPSLTITYTGFVNNEGPSAISQPT
                     IATTATSSSSPGNYLITLTGGASDNYNISLVNGTLTVLTPITCNISMPSTLPLCGIGG
                     NSINATASGGNSYSWNVSSSNNSWSMTGGQNSLCATYQAGSSGVSGTFILTVTNTTYN
                     VSNSCSLTVSSRCDEYCTYVQSFWGSTTATSCTGASSLTLINSALTTPLVNGSGSRLV
                     TIGTSEGSCLRSKMPGGTTYASLPSGCVSCSGATGTSYLSSSKFKNGLLSNTIALGLS
                     IRLQPALGSVRITARYLTTYNATSCSGGTAVPGTKQVYSLPSSVVSALGSANTVNDLL
                     ILANRVLGGACTTPSATDVNTAVSAVLTGFNSCKVLAGFSSSSGGSRETEVEPVPDEV
                     PAYELEPVMVYPNPTDGNATAVFLGGLGQLTTLEVYDLSGSRMATLWNGQTETGLPYL
                     VEIPSAQWAAGIYFVRLTNGSESVVTKLILTDQR"
     gene            4383..>5286
                     /locus_tag="P5292_02360"
     CDS             4383..>5286
                     /locus_tag="P5292_02360"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS37998.1"
                     /translation="MKCSHYLLFIVLLLTHPLFAQDPVADSLKNALATAPADTNRVNL
                     LFTLAKSSMATSPTEALDYSEQMLKLSEQLKFSKGVAQGYKWLGIAKFNLGNYFDAIQ
                     NYEKAQAVFDSIGDRRGVANMYSNIGNVYYNQGEDSKALEYFLQSLKISEEINDTLRT
                     TTALINIGAVFANKSITYPQALKYLGRALPLSLAIDDLTTYGTACVNIGEIWLNSNEY
                     DSAQVYFEKAAKAYDGTEDLPYALNDLGKVYQMRQEYDAAYRYQKQAYDIAKSLDLRN
                     DMAISMLGIAKTLQLRGETRTALNA"
BASE COUNT         1205 a         1651 c         1316 g         1114 t
ORIGIN      
        1 ggatgatgtg accttcctta cctcaccgtt ctatcaggac ggcgtgatcg cgaaagcagt
       61 ggattccgtt gcggcaatgg gtgtcaccta tttcacctcc gccggcaact tcgggaacaa
      121 atcctacgaa ggcgctttca gtccgatggt cgcccccttg ggcatcgcag gtcaggcgca
      181 cgacttcggc ggcaacgaca acctgctcag cgtcagcctg cctccaggca actacaccat
      241 tgtgatgcaa tggcaggatt ccatttactc gcttggccag acatccaccg gtacggtcaa
      301 cgatttcgac atctacctga ccaatcagta cggcacccaa tacttcggat tcaaccgcaa
      361 caacctgggt ggtgatccgc ttgaagtgct gccgttcatc gtcccgggca cttccaatgt
      421 ccaggcgaat atctcggtca tccgtgccgc cggcaaccag gctgcccggt ttaaattgat
      481 cgtctaccgc ggcgagatca ttttcaacga acacaccacc ggaagctcga ccatcaccgg
      541 acagggtaac gcgaacggcg cgatcaccct gggtgcgtcc aactacttca atacgcctcc
      601 cttcggcgtg agccctccct tggtgcagaa tttctcctcg cgcggaggta cgctcgttga
      661 cggcgccatc cgaaacaagc cggatcttgt cgcaccgaac ggtggtaaca cgaccgttaa
      721 tctcggagcc ccgaacgttg acaacgaccc cttcccgaat ttcttcggaa cctcggctgc
      781 tgctccgcat gcggcagcgg tcggcgcact gctccgtcag ggcaaacaga agttttacgg
      841 aaccgacctg agtccgtccg aagtcaggaa cctgttgcaa actacagcgc tcgacatgga
      901 cgcgccgggc ttcgattttg ccagcggcgc cggttacatt caagcctacg cggccttgaa
      961 gaccttcgcg gctccgaccc cggatctcga ttccctggtc gtgcccagcg gcatcacccc
     1021 cggcacctcc accttcaaca ccagcgtgta tggccagttc tttacgacac agtcggtggt
     1081 catgttcaac ggcagcgtgc taccgaccag tttcgtgaat ggaaacgtgc tctcggctac
     1141 cgtacctccc ttcttcggta actacccgct cagtgtttat actcctccca tcgtaccgaa
     1201 catcaacgac ggcggctact ccgacacctt gtatttcttt tcgcctgtca agaagcaggt
     1261 cgtcatcact gccgatgaca agacgaaaaa atacggtgag cagttgccgg agttcacggc
     1321 taccatcacg gtcgacggag ttccactggc gtcttccgga ttcacgctga ccgatctcgg
     1381 actcgaccag cttacgttca cgagttccgc gaccagtgcc agtaataccg gcctcttctt
     1441 catccaacca tccaacgtct tcacggatcc gggataccag accctgttca attactcatt
     1501 cgtgaatggg ctccttacca ttgatccgat gccgatcgtg atcacgcctc gggatacaac
     1561 acttacctac ggcgacaaga tcgaaggatt ccaattcaac tacgactacg acgacaccaa
     1621 catcgatcct tccctgcgcg ccaatttcct gggcgacatc aagcagacgc acaattccca
     1681 gctctccaac gtgctggcgc ttgtggacgg ccgggtggtg acgaatggcc gggtagtcac
     1741 gaatatcgac ctgcagaacc tggccatggt agccagcgga cgggcggtaa cgaacgggcg
     1801 ggtcgtgacg aacggacggg tggtaacgaa cggcatcgag tacgacacga cgattgtcgt
     1861 ggatgtcgcg cccgcttcga ttttctctta tgtcgattcg atggccacct ataccggcga
     1921 caccttgtat gccactttgt acagccctcg cgccggcgtc aacggtcgtg tcgtgaccaa
     1981 tggtcgcgta gtaaccaatg gtcgcgtagt gaccaacgga cgcgtggtga ccaacggacg
     2041 ggtggttacc aatggctatc cggtgctcaa cagttccaac gtgaacctgg gcagcaacga
     2101 taacatggcg atcatcatcg actccacgga catttacgcg gcgcctcccg gcgattcact
     2161 ctatgacctg aagtcgatca acatggtaac cggcaccacg gtcggtacct ggagcatcgt
     2221 tcccggagcc ttcgtgagca gcaacttctc gatttcttac ggcttgggcg aactgacgat
     2281 cgagccggct acgctgacgg tgaaagcgga agacaaatca gctacctatg gcgaatcgcc
     2341 cgccttcacc tatacggttt cgggttatca gtatcaggat gccgacagcc ccgtattcgc
     2401 aggacctccc gtattcgaag tagtcgacgc ccagaatgct gtcgtcaatc cgcccggtgc
     2461 ggggtcgttc ggtgtacgga tccaatccat gccgttgttg cagccatcga attatataac
     2521 gcaattcgag caaggcgatc tggatgtcgc taaggcggac ctccacatca cggctgacga
     2581 taaatcacga acctacggtg cagccaaccc tgccctgacg ctcagctacg cgggcttcgt
     2641 atataacgat ggtcctgcgg ccctcaccgc acctacggct tctacctccg ccgaggcgac
     2701 gagtccggcc ggcaattatc cgatcaccct ttccggcggc caggcagaca actataacct
     2761 cttgttgcac gacggcaact tgcggatcga caaagcgaat ctggaagcca gagccgacaa
     2821 caagaccatg gtaggcggaa cgtctgtccc gtcgttaacg atcacctata ccggattcgt
     2881 gaacaacgaa ggcccatccg ccatttcaca accgacgatc gcaacaacgg ctaccagcag
     2941 ttctagcccg ggcaactatc tcattacgct tacgggcgga gcttctgaca actataacat
     3001 cagcctcgtg aacggaacct tgacggtcct cacgccgatc acctgtaaca tcagcatgcc
     3061 gtcaacccta cccctttgcg gcatcggcgg aaatagcatc aatgctaccg caagcggtgg
     3121 taacagctat tcctggaatg tcagttcttc gaataattcc tggagcatga ccggcggaca
     3181 aaacagcctt tgcgctacct accaggccgg cagcagcgga gtttccggaa ccttcatact
     3241 tacggtaacc aataccacgt ataacgtcag caattcttgt tcacttaccg tgagttcacg
     3301 ttgcgatgaa tactgtacgt atgttcaatc cttctggggc agcaccaccg ccacttcctg
     3361 taccggcgct tcgagcctga cgctgatcaa ttccgcgctc accaccccac tcgtgaacgg
     3421 aagcggttcc agactggtta ccatcggaac ttcagaaggc agttgcctcc gctccaagat
     3481 gcccggtgga acgacgtacg cttcgttgcc gagcggctgc gtatcctgtt ccggcgcaac
     3541 cggaacatcc tacctgagca gcagcaaatt caagaacggt ttgttgtcga atacgattgc
     3601 tttgggtctc agcattcgcc tccagccggc cttgggcagt gttcggatca ccgcccgcta
     3661 cctgacgacc tataacgcga cctcctgcag cggcggcact gccgttcccg gcaccaagca
     3721 ggtttattcg cttccgtcct ccgtcgtttc ggcattggga agcgccaata ccgtgaatga
     3781 tctgctcata ctggccaacc gcgtattggg tggcgcttgc acgacaccat ccgctacgga
     3841 tgtcaacacc gctgtatcgg ctgtattgac cggcttcaac agttgcaaag tactcgccgg
     3901 cttctcgagt tcttcgggcg gcagtcggga aaccgaagtc gaaccggttc cggacgaagt
     3961 tccggcttac gaactcgagc cggtcatggt ctaccctaac ccgaccgatg gtaatgcaac
     4021 cgcggtattc ctgggcggac tgggacaact gacaacgctg gaagtctatg acctcagcgg
     4081 ttcccgtatg gcaacgctct ggaacggaca aaccgaaacc ggcctaccct atctggtgga
     4141 gattccctct gcacaatggg ccgccggaat ctattttgtc cgcctgacca atggttcgga
     4201 gtcggttgtg accaaactga tcctgaccga ccagcgctaa catcagcgac aacatttttc
     4261 caatgcaaaa ggccgtcccg caagacggcc ttttgcattg gaaatacagc ctaaacaccg
     4321 gtttcaatcg ctgaagcttt cattgaaatt gattaaattg tcccatcatt cgccggtact
     4381 tcatgaagtg ttcgcactac cttttattca tcgtcctgct gctcacgcat cccctttttg
     4441 cacaggatcc cgtagccgac agccttaaga acgccttggc aactgcgcca gcggacacca
     4501 atcgtgtgaa cctgcttttc accctagcca agtcgagtat ggctacatcg ccaacggaag
     4561 ccctcgacta cagtgagcaa atgctgaaac tttcggagca actgaaattc agcaaaggtg
     4621 tcgcgcaagg ttacaaatgg ctcggcatcg ccaaattcaa cctcggcaac tatttcgatg
     4681 ccatccagaa ttatgaaaaa gcacaagccg tctttgattc catcggtgac cggcgcggcg
     4741 ttgccaacat gtacagcaac atcggcaatg tctattacaa ccagggtgaa gactcgaaag
     4801 ccctggaata cttcctgcaa tcactcaaga tctcggaaga gatcaacgac accctgcgga
     4861 ccacgaccgc gctgatcaac atcggcgccg tgttcgcaaa caagtccatc acctatcctc
     4921 aggcactgaa atacctcgga cgggccttac cgttgagcct cgcgatcgac gaccttacca
     4981 cctacgggac cgcctgcgtc aacatcggcg agatctggct gaacagcaat gaatacgatt
     5041 ccgcccaggt gtacttcgaa aaagccgcga aggcctatga tggaaccgag gatctgcctt
     5101 atgccctgaa cgacctgggc aaggtctacc agatgcgcca ggaatacgac gcggcctacc
     5161 gctaccaaaa gcaggcctac gacatcgcca agagcctcga cctgcggaac gacatggcca
     5221 tctcgatgct cggcatcgcc aagactcttc aattgcgggg cgaaacccgt accgccctta
     5281 acgcct
//
LOCUS       DAOTON010000072         5277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13072_length_5277_cov_3.714094, whole genome shotgun sequence.
ACCESSION   DAOTON010000072 DAOTON010000000
VERSION     DAOTON010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13072_length_5277_cov_3.714094"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..455
                     /locus_tag="P5292_02365"
     CDS             63..455
                     /locus_tag="P5292_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRS37999.1"
                     /translation="MTRLSSDTVTCPKNPETIFGFLSDFNNFQRLMPDQVTNWQSTSD
                     SCSFTIAGMATLGMRIQEKTPHSSIKVVRDGKAPFDFNLTCTIAPNGTGSDVQLLFDA
                     DLNPMLKMMAERPLTNFLNLLAHKMKEI"
     gene            complement(465..1235)
                     /locus_tag="P5292_02370"
     CDS             complement(465..1235)
                     /locus_tag="P5292_02370"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRS38000.1"
                     /translation="MTRELTTLFTGRRRFDLDSVASTNAYLGDLLRRESLPEGALVCT
                     TVQPEGRGQQGTRWLSAPGKNLLASFYFRPDFLPLSEVFRLNEIFSLAVSDTVASFIT
                     DEVQIKWPNDIYVNGEKVAGLLIENTIQGSRIGASILGVGLNVNETMFPAELPNPVSL
                     AVLTRHQHEIESVVIELCNHLEHRYLQLKQGKTESLRQEFIARQYRRGVWALFSDSNG
                     TFRARFKDITPEGRLCLEHEDGSVVKYDLKSIRYVLDC"
     gene            1342..1758
                     /gene="rsfS"
                     /locus_tag="P5292_02375"
     CDS             1342..1758
                     /gene="rsfS"
                     /locus_tag="P5292_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRS38001.1"
                     /translation="MPKTKKAPARKVAKKSVRKLAEHEQLRDAIIEGMQEKKAKDIVW
                     IDLRNLKNSVADFFVICHADSRTHIESIARSIEEFVYKKLGEEPLHSEGQTNAEWILL
                     DYFTVVAHVFLQDKREFYGLERLWADADIQRIASNY"
     gene            1843..3924
                     /gene="ftsH"
                     /locus_tag="P5292_02380"
     CDS             1843..3924
                     /gene="ftsH"
                     /locus_tag="P5292_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRS38002.1"
                     /translation="MSEEKKNPEQRESGNKGGGKKLPGPPRFNFNFYWIYGIIIVILI
                     LTQLFQWGGTERKTTFDKFERNMLMTKDVDRIVVTNDVAHIYIKPERLKEDKYKEVRT
                     KTWGNIDNPGPHFILNTGPAELFAKKIEEAQKDFPAEERVSLEYEQDNRTLWDMFFTW
                     IPTIAFFVLIWVLVMRRMNTAGGGSQIFNIGKSKAQLFDKELQVKVTFNDVAGLEEAK
                     VEVMEIVDFLKNPKKYTQLGGKIPRGALLVGPPGTGKTLLAKAVAGEAQVPFFSLSGS
                     DFVEMFVGVGASRVRDLFRQAKEKAPCIIFIDEIDAIGRARGKSPSFSANDERESTLN
                     QLLTEMDGFGSNSGVIVLAATNRADILDRALLRPGRFDRQIYVELPDLNGRHAIFNVH
                     LKPLKTNETVDVEFLARQTPGFSGADIANVCNEAALIAARKNKQVIERQDFLDAIDRI
                     IGGLEKKNKIISSQEKKVIAYHEAGHASVSWLLEHAHPLVKVTIVPRGNSLGAAWYLP
                     EERQITTKEQIMDEICAALGGRAAEDIIFGKVSTGALSDLEKITKQAYAMVTIYGLNE
                     KIGNISFYDSTGQQEYNFGKPYSELTAQTIDQEVKKMIDEAYERTRNLLMKNKEKLDQ
                     LAQRLLEREVIFKEDLEQIFGKRQWEDREVPSLKAPSSNGNSTATPPAVQDPAPTVPP
                     EPGPALTGGQA"
     gene            4024..4674
                     /locus_tag="P5292_02385"
     CDS             4024..4674
                     /locus_tag="P5292_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HRS38003.1"
                     /translation="MQESTTREKVLKRIRQALIHKTNPQFPVIDWEKNVYSEGQADSL
                     EEQFAEAFQKIGGRFVYCENELDFAEQLVTLASDSGWKQIHCTEPWLTELFDRVDFPY
                     VRETNEYPEEMVGITGCEALVARLGSILVSSRQGSGRRLFVAPTIHIIVARTHDLVRD
                     LSEALARVKAKYGEQFPSFVASLAGPSRTADIEKTLVSPAHGPRDIYVFLIDTNEA"
     gene            4675..4878
                     /locus_tag="P5292_02390"
     CDS             4675..4878
                     /locus_tag="P5292_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRS38004.1"
                     /translation="MEPQWERVYFTGQIHQAEIAREILEENSIPSVIVDKRDSSYLVG
                     DIELYVRPEDLPLARIILKQHEL"
     gene            4875..>5277
                     /locus_tag="P5292_02395"
     CDS             4875..>5277
                     /locus_tag="P5292_02395"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRS38005.1"
                     /translation="MNNLTQRVLTAIVGASLLIGAIVYSEASFLLILLLITVLSLVEF
                     YRLAEGDGIRPQTSFGLLIAGIVFIPPLLNTFGIALNLLPLLIITPFIVFIRELYTGA
                     EKPFTNIAYTLLGQIYLVAPLFLFYLFGFQGV"
BASE COUNT         1294 a         1535 c         1360 g         1088 t
ORIGIN      
        1 tacactttaa aacaactcga aacccggaac ccgaaacccg gaacccggaa caaataaccc
       61 tcatgacccg cttatcctcc gacaccgtca cctgccccaa gaacccggaa acgatcttcg
      121 gttttctatc cgacttcaac aactttcagc gactgatgcc ggatcaggtt accaactggc
      181 aatccacttc cgatagttgc agttttacca ttgccggaat ggcaacactg ggcatgcgga
      241 tacaggaaaa gacgccccac agcagcatca aagtcgtacg cgacggcaaa gcgccctttg
      301 atttcaacct gacgtgcacc attgcgccga acggcacagg ttctgacgta caattgttgt
      361 ttgatgccga cctgaacccg atgctcaaga tgatggcaga gcgtccgctc accaacttcc
      421 tgaacttgct ggcacataaa atgaaggaga tctgagcggc aagctcagca atcgagtacg
      481 taccggatgg atttcaggtc gtacttcacc accgatccat cctcgtgttc gaggcaaagt
      541 cggccttccg gggtaatgtc cttgaagcgc gcccggaaag tgccgtttga atcgctgaac
      601 aacgcccata ctccccgccg gtattgtctc gcaatgaatt cctgccgcaa gctttccgtt
      661 ttcccttgct tcaactgaag gtagcggtgc tccaggtggt tacacaattc gatcacaacg
      721 ctttcgatct catgctggtg gcgcgtcagg accgcgagtg aaaccggatt cggcaactcg
      781 gcagggaaca tcgtttcgtt cacgttcaat ccgactccca gaatactggc accgatcctg
      841 ctcccctgga tcgtgttctc gatcagcagg ccggcaacct tctcgccatt cacgtagata
      901 tcgttgggcc acttaatctg cacctcgtcc gtgataaatg aagctacggt gtctgaaaca
      961 gccaaactga aaatttcgtt aagccggaag acttccgaca agggcagaaa gtccggacgg
     1021 aagtaaaagc tggccaacag gttctttccg ggagcgctga gccatcgggt gccctgctga
     1081 ccacgtcctt cgggttgtac ggtcgtgcaa acaagtgcac cctccggcag ggattcgcgc
     1141 cggagcagat cgcccaggta ggcattggtc gacgcgacgg agtccagatc aaaccgcctt
     1201 cggcctgtga ataaggttgt taactcgcgt gtcatatcag gaaccggcag aagctgatcc
     1261 cgttgtaatt taacgggtac aataaattgc ttacctttgc gtctctataa ttaattaaaa
     1321 accagccttt cacccacctg aatgcccaaa acaaaaaagg cccctgcgcg taaagttgct
     1381 aaaaaatccg tccgtaaact ggccgaacac gaacagctgc gcgatgcgat catcgagggg
     1441 atgcaggaga agaaggcgaa ggatattgtc tggatcgact tacgcaacct gaaaaattcc
     1501 gttgccgact ttttcgtcat ctgccacgcc gactcccgga cacacattga atccatcgcc
     1561 cgctccatcg aagaatttgt ttacaagaaa ttgggggaag agccgcttca ttccgaagga
     1621 caaacaaatg cggaatggat cctgctggat tattttaccg tcgtcgccca cgtcttcctg
     1681 caggataaac gtgaattcta cgggctggaa cgcctgtggg cagatgccga tatccaacgc
     1741 atcgcaagta actattaaac caaaacattg ttaagctgtc gaccgtctgc gaatccgtgt
     1801 gtttgacacg cggaacggtt gtacggtttc agtacgtcta ccatgagtga ggagaagaaa
     1861 aacccggagc agcgtgagag cggcaataag ggcggcggca agaaactgcc gggtccgcca
     1921 cgtttcaatt tcaatttcta ttggatctat gggatcatca tcgtcatcct gatcctgacc
     1981 cagttgttcc aatggggcgg aaccgaacgc aagaccacct tcgataaatt cgagcgaaac
     2041 atgctcatga cgaaggatgt cgatcggatc gtcgtgacca acgatgtcgc ccacatttac
     2101 atcaagccgg aacggctgaa agaggacaag tacaaggaag tccgcaccaa gacctggggc
     2161 aacatcgaca atccggggcc gcacttcatt ctgaacaccg gtccggctga attattcgcc
     2221 aagaagatcg aagaagccca gaaagatttc cccgcagagg agcgcgtttc actcgaatac
     2281 gagcaagaca accgcaccct ctgggatatg ttcttcacct ggatcccgac gatcgccttc
     2341 ttcgtgctga tctgggtgct ggtcatgcga cgcatgaata ctgccggcgg cggcagccag
     2401 atcttcaaca tcggcaaatc gaaagcgcaa ctgttcgaca aggagttgca agtgaaggtg
     2461 accttcaacg acgttgccgg actcgaagag gcaaaggtcg aagtgatgga aatcgtcgac
     2521 ttcctcaaga acccgaaaaa gtacacccaa ctcggcggca agatcccgcg cggtgcattg
     2581 ctggtcggtc ctcccggaac cgggaaaact ttgctcgcca aagccgtggc cggtgaagcg
     2641 caggtgccgt ttttcagcct gtcgggctcc gatttcgtcg agatgttcgt tggcgtcggc
     2701 gcatcacgcg ttcgcgacct gttccgccag gccaaggaga aagcgccttg tatcatcttc
     2761 attgacgaga tcgacgcgat cggccgcgcg cgcggcaaat caccttcctt cagcgccaac
     2821 gacgaacgcg aaagcacgct caaccagttg cttaccgaga tggacggctt cggcagcaac
     2881 tccggtgtta tcgtgctcgc ggcgaccaac cgcgccgaca tcctcgaccg agcgctgctt
     2941 cgtcccggac gattcgaccg gcagatctac gttgagttgc ccgacctgaa cggacgccat
     3001 gccatcttta acgttcacct gaaaccgctg aagaccaacg aaacggttga tgtggaattt
     3061 ctcgcccgtc agacaccggg cttctccggc gcggacattg ccaacgtctg taacgaagcc
     3121 gcgctcatcg cggctcggaa gaacaaacaa gtcatcgaac gacaggattt cctcgacgcc
     3181 attgaccgta tcattggcgg cctggagaag aagaacaaga tcatctcatc gcaagagaag
     3241 aaggtgatcg cttatcacga ggcgggccac gcttcggtaa gctggttgct cgaacatgct
     3301 catccgctcg tcaaggtgac cattgtcccg cgcggaaatt cactcggtgc cgcctggtac
     3361 ctgccggaag aacggcagat caccacgaag gagcagatca tggacgaaat ctgcgccgcc
     3421 ctcggtggcc gtgccgcgga agacatcatc ttcgggaaag tatcgaccgg agcgctcagc
     3481 gatctggaga agatcaccaa acaggcttac gccatggtga ccatttacgg cttgaacgaa
     3541 aagatcggca acatcagctt ctacgattcg accggccaac aggaatacaa cttcggcaaa
     3601 ccgtacagtg agctcaccgc tcagaccatc gatcaggaag taaagaagat gatcgatgaa
     3661 gcgtacgaaa gaacgcgcaa cctgctcatg aagaacaagg agaagctcga ccaactcgcg
     3721 cagcgactcc tggaacgcga agtcattttc aaggaagacc ttgaacagat cttcggcaag
     3781 cgccagtggg aagaccggga ggtaccgagc ctgaaagctc catcctcgaa cggcaattcc
     3841 acggccacgc ctccggcagt ccaggatccc gctccaaccg ttcctccgga gcccggaccc
     3901 gccctcaccg gagggcaagc ctgaaccaga ctgataggga gcggcgccag tgggcgccgc
     3961 tcttttttta cctccgccaa ttacccaatt cgtatcttcg ttgagttcga acgcgcatag
     4021 cgtatgcaag agagtacgac ccgggaaaaa gtcctcaaac ggatccgcca ggcgctgatc
     4081 cacaagacca atccgcagtt tccggtcatt gactgggaaa agaatgtcta ttcagaaggt
     4141 caagcggaca gcctggaaga acaattcgcc gaagcttttc agaagatcgg cggacgattc
     4201 gtctattgcg agaacgaact cgacttcgcc gagcaactgg ttacgcttgc cagcgactcc
     4261 ggttggaagc agatccattg cactgaaccc tggctgacgg aactctttga ccgggtggat
     4321 ttcccatacg tccgggagac aaatgaatac ccggaagaga tggtcggaat taccggatgt
     4381 gaagccctcg tcgcccggct gggcagcatc ctcgtctcct cccgccaggg ctccggacgc
     4441 agactgttcg ttgcaccgac cattcacatc atcgtagcac gcacgcatga cttggtgcgc
     4501 gacctcagcg aggccctggc ccgggtgaaa gcgaaatacg gtgaacagtt cccttccttt
     4561 gtcgcgtcac ttgccggtcc gagtcgcacc gccgatatcg aaaagacctt ggttagtccg
     4621 gcacacggac cgcgggacat ctatgtgttc ctgatcgaca ccaacgaagc ctgaatggag
     4681 ccgcagtggg agcgtgttta tttcacggga caaattcacc aggccgagat cgctcgtgag
     4741 atcctggaag aaaacagcat tccttccgtc atcgtcgaca aacgggattc cagctatctt
     4801 gtcggtgata ttgaattgta cgtccggccg gaagaccttc cgctcgcccg gatcatcctg
     4861 aagcagcacg aactgtgaac aacctcaccc aacgcgtcct caccgctatc gtcggggcat
     4921 ccctcctgat cggcgcaatt gtttacagcg aggcctcctt cctgctcatc ctgttgttga
     4981 tcacggtcct gtcactggtg gaattctacc ggctcgcgga aggcgacggg atccgtccac
     5041 agacatcctt cggattgttg atcgcgggga tcgtcttcat cccgccgctg ctgaacacat
     5101 tcggcatcgc gctgaatctc ctgcccctgc tgatcattac gcccttcatt gtcttcattc
     5161 gggaattata caccggcgcc gagaagccct tcaccaacat cgcttacacc ctgcttggac
     5221 agatctacct ggtcgctccg ctatttctct tttacctctt cggtttccag ggagttg
//
LOCUS       DAOTON010000073         5271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13088_length_5271_cov_3.189609, whole genome shotgun sequence.
ACCESSION   DAOTON010000073 DAOTON010000000
VERSION     DAOTON010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13088_length_5271_cov_3.189609"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="P5292_02400"
     CDS             complement(<1..999)
                     /locus_tag="P5292_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRS38006.1"
                     /translation="MSEPLQRRNFLSRFALGLTTALAAPLVAASKKLGGDAENTSPMP
                     AIRKIKPLGFQWETADPFLFCVHHEDQFPKGNMEMGPACSLQGRHLGDDFIIKDGFRM
                     YHGKKVPGFPGHPHRGFETITVVRKGLVDHADSMGAAGRYGNGDVQWMTAGKGVQHSE
                     MFPLLSQEKENPMELFQIWLNLPARNKMVEPHFKMLWRDSIPNYVHRDKAGKKTVVEI
                     IAGKIGDKRAPAPPPDSWAAPEQHEVAVWNIKMEAGSSWTLPAASKGVNRTIYCYEGE
                     GIRVAATAIPRYNAVELEPDAIVEIVAGDKPASILLLQGRPIGETVIQYGPFVMNTK"
     gene            complement(1488..1562)
                     /locus_tag="P5292_02405"
     tRNA            complement(1488..1562)
                     /locus_tag="P5292_02405"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Val,seq:gac)
     gene            1695..2597
                     /locus_tag="P5292_02410"
     CDS             1695..2597
                     /locus_tag="P5292_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HRS38007.1"
                     /translation="MASLISDHPFRSPLAEQETGLCLVDESLSNHTAGTCDLFALFCG
                     GGLSISLQERRSRKFLALETLPNAEQPGRSSAEQFRLAVNESRILKEFEFSRSFLGFG
                     NAWYTLVPNGLFQQGDETRLLRFTQSRSDLQAFHQRIQGFDARLIFGLEPELYHMAAH
                     LFQNPVVLHAVGAQLERASLESSIGSQPTVSLHLSQRELDILVYSGKKLLLQNSFPVN
                     TADEVLYYTLFVLERLELMPDQVNLEIFGSADPRSGALLRLKDHLPQLRVAGWPDYFQ
                     ASYKLLEVPAYRHLDLFSLSLCAS"
     gene            2585..3133
                     /locus_tag="P5292_02415"
     CDS             2585..3133
                     /locus_tag="P5292_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009392898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRS38008.1"
                     /translation="MRIIGGSRKGRMLQAPAKLPVRPTTDYAKTGLFNILSNRIDFES
                     VSVLDLFCGTGGISFEFASRGSLSITAVDQHAGCVRFVQETARQLDFPGIRTLKADVF
                     RYLKQPAIPYDIVFADPPFELEESDRLPELVLNNGWLAPEGMFILEHQAKRKIVSVYE
                     PAEVRVYGNCAFSFYTFKPVQD"
     gene            3168..3626
                     /gene="coaD"
                     /locus_tag="P5292_02420"
     CDS             3168..3626
                     /gene="coaD"
                     /locus_tag="P5292_02420"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020081743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRS38009.1"
                     /translation="MKKIAVFPGSFDPITLGHESIVRRCLPLFDEIVIGIGYNSNKHY
                     FFSQDRREHFIRQTFADASNIRVERYSGLTVDFCKSIGANYILRGLRTSADYEFERAI
                     AQMNRALHPGVDTIFVVADPHLSHISSTIVRDILLYQGDVSQFVPKSVTL"
     gene            complement(3631..4263)
                     /locus_tag="P5292_02425"
     CDS             complement(3631..4263)
                     /locus_tag="P5292_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRS38010.1"
                     /translation="MIHTAYLHDKPLHFVEVYNAGGTDFQPGYLITSEQEMTLEQAIR
                     ELSKEKSPNGILYLCEHPDTVWLQFCHMFTLVEASGGVVENADGELLAIFRKGKWDLP
                     KGKVDGDESPEEAGIREVEEECGITSPVIESELRPTFHTYEEKGRRLLKKTHWYRMRY
                     EGNEALLPQLEESITDARWMSRDEFSRVFLGNTYASLRAMLERYLKSEKA"
     gene            4523..5167
                     /gene="pyrE"
                     /locus_tag="P5292_02430"
     CDS             4523..5167
                     /gene="pyrE"
                     /locus_tag="P5292_02430"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRS38011.1"
                     /translation="MSQRDDTAHTIAEHLLQIKAIRLQPEEPFTWASGWKSPIYCDNR
                     IALSYPRIRTYIRQELVKAIETRFGKPEVIAGVATAAIPQGALVAEALGLPFVYVRSS
                     PKDHGRQNLIEGELKPGQTVVVIEDLVSTGKSSLKAVEALREAGCTVKGLLAVFSYGL
                     KKAEENFRTAHCPFYSLSNYEVLLEKAVETKAVSSQHLEVLRQWRHDPEHWNTK"
BASE COUNT         1194 a         1489 c         1339 g         1249 t
ORIGIN      
        1 cttcgtgttc atcacgaagg gaccgtattg gatgaccgtt tcgccgatcg gacgaccctg
       61 caataacagg atgcttgcag gcttatcgcc tgcaacgatt tcaacgatgg catccggctc
      121 caattcgacg gcattgtagc gcgggatcgc cgtcgcagct acgcgaatgc cctctccttc
      181 gtagcagtag atcgtacggt tgacaccctt ggatgcagcc ggaagtgtcc agctgctacc
      241 cgcctccatc ttgatattcc ataccgctac ctcgtgttgt tccggtgcgg cccacgaatc
      301 gggcggcggc gctggagcgc gcttgtcgcc gatctttccg gcgatgattt ctaccactgt
      361 tttctttcca gctttatccc gatgcacgta gttcggaatg ctgtcgcgcc acaacatctt
      421 gaagtgcggc tccaccatct tgtttcgtgc cgggaggttc agccagatct ggaagagttc
      481 catcggattc tccttctcct ggctgagcaa gggaaacatc tccgaatgct gcacgccttt
      541 cccggcggtc atccattgta catctccatt gccatagcgt cctgccgcgc ccatcgaatc
      601 ggcatggtcg accaggcctt tccgtacaac cgtaatggtt tcaaatccgc ggtgcgggtg
      661 cccggggaag cctggcactt tcttaccatg gtacatgcgg aatccgtcct tgataatgaa
      721 atcatcgccc aggtgacggc cctgcagtga acaggcgggg cccatttcca tgttcccttt
      781 ggggaactgg tcttcgtgat ggacacaaaa gagaaagggg tccgcggttt cccattggaa
      841 tcccaggggt ttgattttcc ggatggctgg catgggggag gtgttttctg catcgcctcc
      901 gagttttttg gaagcagcga ccaaaggagc cgccagggcg gtcgtcagcc ccagggcgaa
      961 gcgcgaaagg aagttccggc gttggagtgg ctcactcatg attcaggatg ttttaggaaa
     1021 ataaccgtgt ggcgaggcaa gtgtatagtt acaaaaaaca gttcaatcgg tcaatagttt
     1081 tcccttcttc tgttcactcg cactgtttct tacactcaca ctaccccttt cttagcatta
     1141 gtgcggagtg tgagaaacag tgtgggtgag cagaaaaaca ctgtacactc acactgtttc
     1201 ttacactcac actacccttc cgtggaatta gtgcggagtg tgagaatcag ggtgggtgag
     1261 cagaaaaaca ctgttcactc acactgtttc ttactctcac actacccttt cttagcatta
     1321 gtgcggagtg tgagaaacag ggtgggtgag cagaaaaaca ctgtacactc acactgtttc
     1381 ttacgctcac acaaaccttc cgtggcaata gtgcgggtgt gagaaacagg gtgagtgtac
     1441 agttaaaaaa aaagcccccc ggagctaacc aggaggcctt atcgttgtgg acgctacagg
     1501 attcgaacct gtgaccccta ccctgtcaag gtaatgctct aaaccaactg agctaagcgt
     1561 ccgggcaccc cacggtgagg tgggggcgca aaagtagaaa attatccaac gccagaaaat
     1621 cctgccggct ttcctacccc aaaaaagcac agtacgacgg gtttttttag ctttggaggc
     1681 cgattcacca tgtcatggcc agcctgatct ccgaccaccc gtttcgttcg ccactggctg
     1741 aacaggaaac cggcctttgc ctggtagacg aatcactctc gaaccataca gccggcacgt
     1801 gcgatctctt cgcattgttc tgcggcggcg ggttgagtat cagcctgcag gaacgacgta
     1861 gccgcaagtt cctggcgctc gaaacgctac ctaatgcgga gcaaccgggc agatcctccg
     1921 ccgaacagtt tcgacttgcg gtgaacgaga gtcggatcct caaggaattc gaatttagcc
     1981 ggagtttcct gggattcggt aacgcctggt acaccctggt gccaaacggt ctcttccagc
     2041 agggtgacga aacgcgcctg cttcgcttta cccaaagccg cagcgatctg caagcgttcc
     2101 atcaacgcat tcagggcttt gatgcccgtc tgattttcgg actcgaaccc gaactctacc
     2161 acatggccgc gcatctgttt caaaacccgg tcgtgctgca tgcggtaggc gctcagttgg
     2221 aacgcgcctc ccttgaatcg agcattggta gccaaccaac tgtttccctg cacctcagtc
     2281 agcgggaact cgatatcctg gtgtacagtg gaaagaaact gctcctgcaa aattcctttc
     2341 cggtcaatac cgccgacgaa gtgctgtatt acaccttgtt cgtactggaa cggctggagt
     2401 tgatgccgga tcaggtaaac ctggagatct tcggaagcgc tgacccgcgc agtggagccc
     2461 ttcttcggct gaaagaccat cttcctcagc ttcgagtggc cggatggccc gactattttc
     2521 aagccagcta taaactgctc gaagtgccgg cctaccgtca cctcgatcta ttcagcctga
     2581 gcctgtgcgc atcataggag gcagtcgcaa ggggcgcatg ctccaggcgc cggccaagtt
     2641 gcccgtccgc ccgactaccg actacgccaa gaccgggctg ttcaacattc tgagcaaccg
     2701 catcgacttc gaatccgtca gcgtcctgga cctgttttgc ggaacagggg gtatcagttt
     2761 cgaattcgct tcccgtggtt cactttccat tacggctgtg gaccagcatg caggttgtgt
     2821 gcgcttcgtt caggaaacgg cgcgacagct ggactttccc ggcatccgga cccttaaagc
     2881 ggatgtgttt cgttacctca aacagcccgc cattccctat gacatcgtct tcgcggaccc
     2941 gccttttgaa ctggaggaat ccgaccgtct gccggaactg gttttgaaca acggctggct
     3001 ggcacctgag ggtatgttca ttctagaaca ccaggctaaa cggaaaatcg tatccgttta
     3061 cgaaccggca gaagtgcgcg tttacgggaa ctgcgcattt tccttctaca ctttcaaacc
     3121 tgtacaagac taagcgtttc gtaacttcgt tcactcgcgc aagcaccatg aaaaagatcg
     3181 ccgttttccc cggctccttc gatccgatca cgctgggtca cgagagcatc gttcgtcgtt
     3241 gtctgccact cttcgacgag attgtgatcg gcatcgggta taactcgaac aaacactatt
     3301 tcttttcgca ggatcggcgg gaacatttca tccggcagac cttcgccgac gcctccaaca
     3361 tccgggtgga gcgctatagc ggtcttaccg ttgatttctg caaatcgatc ggggcgaatt
     3421 acatcctgcg cgggctccgc acgtcggctg attacgaatt cgaacgcgcc atcgcccaga
     3481 tgaaccgggc gctgcatccg ggcgtcgaca cgatctttgt cgtggcggac ccccacttgt
     3541 cacacatttc gtcaacgatt gttcgcgaca tcctgcttta tcagggagac gtctcgcaat
     3601 tcgtcccgaa gtcggtgacc ctttgatcga tcaggctttt tcggacttga gataccgttc
     3661 cagcatcgca cgcaggctgg cataggtgtt ccccaggaac acacgggaaa actcatcacg
     3721 cgacatccag cgtgcatccg tgatggactc ctcgagctgg ggcaacagcg cttcgttgcc
     3781 ttcgtaacgc atgcggtacc aatgcgtctt tttcaacaag cgccggcctt tttcctcgta
     3841 cgtatgaaac gtaggacgaa gttccgattc aatgaccggt gacgtgattc cacattcctc
     3901 ctctacttca cgaatacctg cttcctcagg actttcatcc ccgtcaacct tgcccttggg
     3961 aagatcccat ttacctttcc ggaagatcgc cagtagctcg ccatcagcgt tttccacaac
     4021 acctccggaa gcttctacaa gggtgaacat gtggcagaac tgaagccaaa ccgtatcggg
     4081 atgctcacag aggtagagaa taccgttcgg cgacttctcc ttgctcaact cgcggatggc
     4141 ctgctccagc gtcatttcct gttcactggt gatcaggtag ccgggttgaa aatcagtacc
     4201 accggcgttg tacacttcta cgaagtgcaa gggtttatca tgcagataag cagtgtgaat
     4261 cacatgagga agataaatac gattcaggaa gattggtaat gatgagcgaa aattaatttg
     4321 aatccttctg ttgccactag tggaactccc catttaatcc attgatcaac attccggacc
     4381 caaacaattt ttggtcatcg taaatcccgt gacaagctct catccgaagt tcgtaggcag
     4441 gaaccgtgtg tacagtgggg ttgctgcaag gtcattcaac cgtcaatcag taggaaacgc
     4501 ccgaattgcg tagttttgca ccatgtccca gcgcgacgac accgcccaca ccatcgcgga
     4561 gcacctgctt caaatcaaag ccattcgcct gcaacccgaa gaacccttta cctgggcgtc
     4621 gggctggaaa tcacccatct actgcgacaa caggatcgcc ttgtcttatc caaggatccg
     4681 cacctacatc cgacaagagc tggtcaaagc catcgaaaca cgattcggca agccggaagt
     4741 gattgcaggt gtcgcgactg ctgcgattcc gcaaggagcg ctcgtagcgg aagcattggg
     4801 gttaccattc gtttacgtcc gctcctcgcc taaagaccac ggccgtcaga atctcatcga
     4861 aggcgaattg aaacccggac agaccgtcgt tgtgatcgaa gacctcgtct ccaccggaaa
     4921 atccagtctg aaagcggtag aggccttgcg tgaagccggc tgcaccgtga aaggattgtt
     4981 ggccgtgttc tcttacggat tgaaaaaagc cgaggagaat ttccggaccg ctcactgccc
     5041 gttttactcg ctcagcaact acgaagtgct cctggaaaaa gccgttgaaa caaaagcggt
     5101 atcctcccaa caccttgaag ttctccgcca atggcgacac gatcctgaac attggaacac
     5161 taaatgagag ttgttggttt cgagtttcga gttccgggtt ccgggttgga aattgaaatc
     5221 tgcagcgcta cctgatgcta ttgcggtcgc tcttaccatt acactttaaa a
//
LOCUS       DAOTON010000074         5267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13098_length_5267_cov_3.363584, whole genome shotgun sequence.
ACCESSION   DAOTON010000074 DAOTON010000000
VERSION     DAOTON010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13098_length_5267_cov_3.363584"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="P5292_02435"
     CDS             complement(<1..883)
                     /locus_tag="P5292_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38012.1"
                     /translation="MRTLGVLFLLIGLSGPAAASAVVDLQGRLLNRADSLPLAFAVVR
                     EPTSGIGTTADIDGRFDIRLEVETGSVLLEVTRVGCRTKHWVSMRNTTPIVLYIDCPA
                     LNIREVGIRGYTAEQMVREAVSRIPGNTAAFTYVTPGRARRCRTVNGKITALTESELM
                     VLYNARDDSGKVSEAVAMAMLDDRQPVPEGEDHRTRDRYELLFAADPVLRLKENLLNP
                     AAFPEYRFDFLDSGTGDTVRIRYVCESLENESHGIENYHEVELREEGWELGEITLLQS
                     SFAVLSVKREAFRRAAYQ"
     gene            complement(1029..4502)
                     /locus_tag="P5292_02440"
     CDS             complement(1029..4502)
                     /locus_tag="P5292_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38013.1"
                     /translation="MKCLLAGFLYLGWLSAGAQVTVGSGTTDQGYSLNTGAVYVDPNL
                     QITSLTPISGFKVTISSGFQSGDVLSFTEPLPLGVNDSWDGNTGVLTFTGSATAEDWQ
                     TLLRSVTFENANDWEVGDRTITFSAGSLSAFSNGHFYELVDNGGDDWNMARANAEART
                     YLGKTGYLATITSQAENDFIAQVLAADAWIGAADEYTLINDATGTTTYPDQWSAEGNW
                     YWVTGPEAGTLFSVGNEFPATVPGEYANWNDWEPNDSGGEHFGQIYSSGAYGRWNDLP
                     DWVGLAFVVEYGDMPGDPATSISATTRIYINVTGNTVANAQTICYNASAAALTGSNPG
                     GGTGSYTYQWVRSTSGASGTYTAAPGTNNDIDYSPGTLTQSSWYRRVVTSGSAVDSSS
                     AILVTVNPSISFSSSTTAVSCNGGSNGVAAVTVVGGTSPYTYSWTGGGSSATRTGLAA
                     GNYVCTITDAANCTTAAHFTVTQPTAVSATTSQTNVACNGAATGVAAVSATGGTTPYS
                     YQWSSNASTTSSVSSLPAGSYTCTITDANACTLTRTFTITQPTAISAASATLQHLSCN
                     GANDGSILLSATGGTGSLSYAWSSGASGNNPTGLSAGSYTCTVQDANNCTAQFSYTIT
                     QPAAMNASAATTDATCNGASDGEATVTATGGTAPYTYSWSSGASSQTAAGLAAGNYTV
                     TITDDNGCTTTQQATIAQPTAVQTALSATATSCYGSSNGEAHAMTSGGTAPYSYDWSN
                     GASASALANIPAGTYSVTVTDANGCTTTAQVDVNAPDEIQATTSAVALSCAGDASGEA
                     AIAASGGTGALTYLWSNGATTTTMNGLDAGNYTVIVTDANNCTRSFSVNVSEPAALNA
                     GIQTTDAACGQANGSASASVAGGTGPYQYEWSTGTMGQYATGFSAGNYAIEITDAHGC
                     QTYQSFVINNEDAPMVAATTQGPSCHGGSNGTIDMQIWTSAGGYTLLWDDGFTGTSLT
                     GLSAGVYGYKVIDANGCTTIGSVSVNEPQAVSVSATVNDATGTGNGSIDLTVDGGTPA
                     YSYLWSNGATTEDLNNLLPYDYTVTVTDANGCTATQLFSVGTTTALGPDVSDKGQFVF
                     PNPNNGYFRIQGHADAVYQLFDVSGKLVRAFDLRGKSDSVLDVSSLGKGIYFLRETDA
                     RGVRAERIIVQ"
     gene            complement(4929..>5267)
                     /locus_tag="P5292_02445"
     CDS             complement(4929..>5267)
                     /locus_tag="P5292_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38014.1"
                     /translation="KWVYGLFQSFRLSWLVVIIGLFFALQRKNWNAVLFIFLPVALAA
                     SQYYFAYDLSRLGGLAYPAFFVAIDQLRKAHPTWFAPLAIALVLANFFVPAYCVGALD
                     PLWYGPFWMK"
BASE COUNT         1130 a         1463 c         1567 g         1107 t
ORIGIN      
        1 actgataagc ggcccgacga aaagcctcgc gtttaacgga gagcaccgca aagcttgatt
       61 gaagcagcgt gatctcgccg agctcccaac cttcctcccg cagctccact tcgtgatagt
      121 tctcgatgcc gtggctttcg ttctcgagcg actcgcatac atagcgaata cgcaccgtat
      181 cgccggttcc gctatccaaa aaatcgaacc gatactccgg aaaggccgcg gggttgagca
      241 ggttctcctt caggcgaagg accggatcgg cagcgaacag caattcgtag cgatcacgtg
      301 tacgatgatc ttccccttcc ggcacgggtt gccggtcatc caacatggcc atggcaaccg
      361 cttcgctcac cttaccgctg tcatcccggg cgttatacag gaccatcaac tccgattcgg
      421 tcagggcggt gatctttccg ttcacggtcc gacaacgcct cgcccggccc ggcgttacat
      481 acgtgaaggc cgcggtattg cccggaatcc gactgacggc ttcccgaacc atttgctcgg
      541 ctgtatatcc ccggatgccg acttcccgga tgttcaaggc cggacaatcg atgtagagta
      601 cgatgggtgt tgtgttccgc atcgacaccc agtgcttcgt ccggcatccc acccgcgtca
      661 cctccagcaa gacggagccc gtctccactt ccagccgtat atcgaagcga ccgtcgatgt
      721 ctgcggtggt tccgatgccg ctggtcggtt cccgaacaac ggcaaaagcc aggggcaagg
      781 aatcggcgcg gttaagcaac cgtccttgca aatccactac agctgatgca gcagccggcc
      841 cggagagtcc gatcagcagg aatagcacac ccaacgtccg cattcccgaa tatactgaat
      901 cgacgacgga tggggctacg cccggtagtc accgataaaa agcaaacggc ctgaagcaag
      961 acttcaggcc gtttgatctg gtatccggct cgattacacc ctcatgagcc gaatcaaatg
     1021 aacgatcctt attgtacgat gatccgctcg gcacgaacgc cacgggcatc cgtctcgcgc
     1081 aggaagtaaa tgcctttacc aagcgaggac acgtcgagca cactatcgga tttaccacga
     1141 aggtcgaatg cacggacgag ttttcccgac acgtcgaaca actggtaaac cgcgtccgca
     1201 tgaccctgga tccggaagta gccgttgttc gggttcggga atacgaattg acctttatcg
     1261 gatacgtcgg ggcccagtgc ggtagtggtg ccgaccgaga agagttgcgt agcggtacag
     1321 ccgttcgcgt cggtgacggt cacggtgtaa tcgtacggca ggaggttgtt caggtcttcg
     1381 gttgtagcgc cgttgctcca gagatacgaa taagccgggg ttccgccgtc gacggtgagg
     1441 tcgatcgagc cgttgccggt tcccgttgca tcattgaccg tagcggaaac cgaaaccgct
     1501 tgcggctcat tcaccgatac ggaaccgatg gtggtacaac cgttcgcgtc gatcactttg
     1561 taaccgtaca ctccggctga aagtccggtc agggatgtgc cggtaaagcc atcatcccac
     1621 agcagcgtat agccaccggc gctggtccag atctgcatat cgatggtacc gttgctgcca
     1681 ccgtggcagc tagggccttg cgtcgtagcg gcgaccatcg gagcatcttc attgttgatc
     1741 acaaacgact ggtacgtctg gcaaccgtgc gcatcggtga tttcgatcgc gtaattgccg
     1801 gcactaaaac cggttgcata ttgtcccatg gtaccggttg accattcgta ctggtacggg
     1861 ccggttccac ccgctaccga agccgaggcg gagccgttgg cctggccgca agcagcgtcg
     1921 gttgtttgaa tgcccgcatt cagagcagca ggttcggaga cattgaccga gaaggagcgt
     1981 gtgcagttgt tcgcgtcggt tacgataacg gtatagttac cggcatcgag gccgttcatg
     2041 gtcgtggtgg tagcaccgtt cgaccagagg tacgtcagcg ctccggttcc gccgctggct
     2101 gcaatcgcag cttcaccgct ggcatcgccg gcgcagctca gcgcgacagc gctcgtggtg
     2161 gcctggatct catccggggc gttgacatcc acttgcgcgg ttgtcgtgca gccgtttgcg
     2221 tccgtgacgg taaccgaata cgttcctgcc gggatgttgg caagggccga ggcgctggca
     2281 ccattcgacc aatcgtagga atatggagcc gtaccgccgg aagtcatggc atgcgcttct
     2341 ccgttggagg aaccgtagca cgaggtagcc gtagcgctca gtgctgtttg taccgcggtc
     2401 ggttgtgcga tcgtcgcttg ttgagtcgtc gtacaaccgt tgtcgtcggt gatggtcacg
     2461 gtatagttac cggcagcaag gccggcagcc gtttgactgg aagcgccact gctccaggag
     2521 taagtgtatg gagcagtacc accggtggcg gtcacggtcg cttcaccgtc gctggcgccg
     2581 ttacaagtag catcagtcgt tgcagcggaa gcgttcatcg cggccggctg ggtaatggtg
     2641 tagctgaatt gcgcggtgca gttgttggca tcctggaccg tgcaggtgta gctgccggcg
     2701 gaaagtccgg tcgggttgtt accactcgcg ccggatgacc atgcatacga caagctgccg
     2761 gtcccgccgg ttgcggacag gaggatcgaa ccatcgttgg cgccattgca actcaggtgt
     2821 tggagcgtag cgcttgcagc ggagatggcc gtcggctgtg tgatcgtaaa ggtgcgggtc
     2881 agggtacacg cgttcgcgtc ggtgatcgtg caggtgtaag atcctgccgg caggctggaa
     2941 acggatgaag tggtcgaagc attcgacgac cattgatagc tatagggcgt cgtacctccg
     3001 gttgcactca ccgcggcaac gcccgtggcc gcgccattac aagcaacgtt ggtctgcgag
     3061 gtcgtagcgc ttaccgccgt gggttgcgtg acggtgaaat gagctgccgt cgtacagttg
     3121 gccgcatcgg tgatcgtgca gacatagttg ccggcagcca gtccggtacg ggtggcggaa
     3181 gagccgccgc cggtccagga gtaagtatag ggtgatgtgc caccgaccac cgttacagcc
     3241 gcaacaccgt tgctgccgcc attacaggag acggcagtag tgctcgacga aaaactgatc
     3301 gacggattaa cggttaccag gatagccgaa gaggaatcca ccgccgagcc ggaagtcacc
     3361 acacgacggt accacgaaga ttgcgtgagc gtacccgggc tgtagtcgat gtcgttgttg
     3421 gtgccgggcg cggccgtata ggtgccggaa gcgccggaag tagaacggac ccattgataa
     3481 gtatatgatc ccgttccgcc gccgggattc gatccggtca gcgcagcagc ggaagcattg
     3541 tagcaaatgg tctgcgcatt ggcgaccgta ttgcccgtta cattgatata aatgcgcgtg
     3601 gttgcggaaa tgctggtggc aggatcgccc ggcatatcgc cgtactcaac gacgaaagcc
     3661 agccctaccc aatccgggag gtcgttccaa cggccgtagg ccccgcttga atagatctgt
     3721 ccgaagtgtt cgccacccga atcgttgggt tcccaatcgt tccagttggc gtattctccc
     3781 ggtacggtgg ccgggaactc gtttcctacg gaaaacaagg tgccggcttc cggacccgtt
     3841 acccaatacc agttaccttc ggcggaccac tgatccgggt aggtggtcgt tccggtcgcg
     3901 tcgttgatca gggtgtattc atccgccgca ccgatccagg cgtcggcagc gagtacctgc
     3961 gcgatgaaat cattctccgc ttgtgaggtg atggtcgcga ggtagccggt ctttccaagg
     4021 taggtgcgtg cttccgcgtt cgccctggcc atattccagt catcaccgcc gttatcgacc
     4081 agctcgtaaa agtggccgtt actgaaagct gacaagctgc ctgcactgaa agtgatcgta
     4141 cgatcgccta cttcccagtc gttggcattt tcgaaggtga ccgaacgcaa cagcgtttgc
     4201 cagtcttcgg ctgtggcgga cccggtgaag gtgagcacac cggtgttacc gtcccaggag
     4261 tcgttcaccc ccagtggaag tggttccgtg aaggaaagga cgtcgccgga ctgaaagccg
     4321 gaggagatcg tcaccttgaa gccgctgatc ggtgtcagcg aggtgatctg caggttcggg
     4381 tcgacgtaca cggcgccggt gttcagggaa taaccctgat ccgtggtgcc ggaaccgacc
     4441 gtgacctgcg cgcctgcgct cagccatcca agatagagga agccggcgag caggcatttc
     4501 atgattttcg tttgaaattt gttcatgggc gtagaacagt tagtcaagga tgtgtttaga
     4561 ttttgactct gatacgctca ctggtgccgc taccgatact cgtcattcac gcgccgcctt
     4621 tcaatgtgat gatgtgtaag aattaacgtg aataaacgca tggttttttc ccaatgcggg
     4681 acggaccttc cttgatttaa tcagaatccg cccttgcacg atgcttgttt cggcctaagg
     4741 aagttttaaa agtgaatgga attggtgaaa tcgttcacgc aattccgttc atgcgcgagg
     4801 attcgaacat tcattacagt ttaatcaaga catttcctgc ctttaccagg atgctgaaag
     4861 caggcagccg gctgtgcaat tgcacaggta ctattccgtt aacgactcca cgtttaagga
     4921 aaggacaatt acttcatcca aaacggccca taccacaaag ggtccagggc accgacgcag
     4981 taagccggga cgaaaaagtt ggccaggact aaagcaattg ccaagggggc gaaccaggtc
     5041 ggatgcgctt tgcgcagttg atcaatagcc acgaaaaaag ccgggtatgc cagaccgccc
     5101 aggcgggaaa gatcataggc gaaataatac tgcgaagcgg cgagtgcgac gggaagaaaa
     5161 ataaacagga cggcattcca gtttttccgc tgcagggcga agaacaatcc gatgatcacc
     5221 acgagccacg acaaacggaa cgactggaac aagccgtaca cccattt
//
LOCUS       DAOTON010000075         5263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13106_length_5263_cov_4.113863, whole genome shotgun sequence.
ACCESSION   DAOTON010000075 DAOTON010000000
VERSION     DAOTON010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13106_length_5263_cov_4.113863"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="P5292_02450"
     CDS             complement(<1..266)
                     /locus_tag="P5292_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRS38015.1"
                     /translation="MKAQQEINRHIATHPAPKRSELSTLHELVLSVDPGCKLWYYDGK
                     DEQGKVVANPTIGYGTQVLRYANGKTREWFRIGISGNKTGISVYL"
     gene            complement(306..725)
                     /locus_tag="P5292_02455"
     CDS             complement(306..725)
                     /locus_tag="P5292_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRS38016.1"
                     /translation="MATLHPHINFNGNAREAFEFYRSVFGGEFSRILYLKELAGPDVP
                     IADEDADKLLHIALPVGTGMLIGNDVPSFLGRVNEQENRSKISVRAVSREEADRLYHG
                     LSQGGTVEAPIGESPWGTYFGMFRDKYGIEWIVETDR"
     gene            complement(864..1088)
                     /locus_tag="P5292_02460"
     CDS             complement(864..1088)
                     /locus_tag="P5292_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRS38017.1"
                     /translation="MAIVVNLDVMMAKRKMSLNELSEKVDLTLSNLSILKTGKAKAVR
                     FSTLEAICKALNCQPGDLLEYVADREGEIE"
     gene            complement(1092..1628)
                     /locus_tag="P5292_02465"
     CDS             complement(1092..1628)
                     /locus_tag="P5292_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HRS38018.1"
                     /translation="MSKTNSLVFKALYIIAWFIFVGLSIEAGGLVVNFFFHLYRPDFV
                     VNLYQKLDLREMLTQSRASFFGIYSLILSIALLKSFLFYIVIRLMHTMNLSKPFDLFV
                     SSQILRISYFTLSVGFLSYIARQFARNLQQHGLITDGLHQFWEDSQAFVLMGAVIYII
                     AAIFKRGVDIQSENDLTV"
     gene            complement(1927..3972)
                     /locus_tag="P5292_02470"
     CDS             complement(1927..3972)
                     /locus_tag="P5292_02470"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRS38019.1"
                     /translation="MKIVLVSLLIFFCQVSQAQQQHACGHAEMEELLLQKHPALLHER
                     AALEQFTATYTAHRQQRVLSDTILFIIPVVFHIMHNYGEENISREQIVDAVRIMNEYY
                     QKRNADTAQIIPLFQPLIGDVRLEFRLAQLDPQGNCTDGITRHQTYLTNGGNDLLKSI
                     LQWPPDRYLNIWVEKNTLNSFSAYAYLPGAPPALDGIVVTHPYVGSIGTAQGSSSIAI
                     LAHEAGHYFNLLHTWGPTNTPGLASNCSLDDQVFDTPNCIGGVCNLNAPGCIAGEISN
                     VQNIMDYCFEQMFTLGQVTRMQAALNAPTGNRNNLWSTGNLALTGTNDGYIPVACPPI
                     ADLTNRVMRICEGQSITFENLSYGGNVDSVEWQFTGGNIVSSTDPSPTVQYPVAGIHD
                     VVLTVFNGSGSSSIVRNGLVNVLPAVPSLTAPVTQDFESLQFPSGAWGVENAAGSAWE
                     ITTNASVSGSHSIFLQRDSLNRETDDVIYTESYDFSNCPSPTFSFRLAFANVNASGDV
                     LKIFMSTDCGQTWNMRYSKSGSFLATATDTAYFFIPSSGQWRQDGANINGAAGEAQVC
                     FKFQFTSKGGNSIYVDDINIGGLPVKVQTMEDVDLIQLKPNPAYQQAILILDLPLSAN
                     ISYTILDVAGRAVRSNDMQLLTAGKHAFEISRPPAAGLYFLLVRVNDQLSLRKLVFE"
     gene            complement(3991..>5263)
                     /locus_tag="P5292_02475"
     CDS             complement(3991..>5263)
                     /locus_tag="P5292_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRS38020.1"
                     /translation="DTASKITKVLEPFEHYGWSIHDPLVDPTFGSAVASVHSGSSSRL
                     FLLGGYGQLTILPDAYEYDLGFNSWTSIAPMPVECTYASSATANGKIFVFGGRTLGTA
                     QGSTQIYDYATDTWSTGSPMPTPVITAAYGTYRDSLVYIIGGLIDATGGAVSLVQIYN
                     AYTDTWSSGTTLPGPALYASRAGIVNNKIVVAGGYNPVTGQATASTYVGEINVADPLQ
                     VNWTQADDHPAGMLSRGGAATSLDPASGLVVFAGGSPEPSIFTTTDRTFAYDVNSGSW
                     KLGPDKPTARNLFYMTPILYQDSVYLVALGGNPPGTSLNHSDAHEWLNLGRYSLATGI
                     VTMENIAGVSVFPNPFQDGFNVVLELPKPMRCKITLTDVLGRELKVVSDGRLPGGRVT
                     VPVSLPDVPAGLYSCTIQAEGNTLYTKVVKY"
BASE COUNT         1290 a         1375 c         1377 g         1221 t
ORIGIN      
        1 aggtaaaccg aaatccctgt cttgttgcca ctgatcccga tccggaacca ttcccgcgtc
       61 ttgccattcg cataacgcaa tacctgcgtg ccgtacccga tggtcggatt ggcgacgact
      121 ttaccctgct cgtcctttcc gtcgtaatac cacaacttgc agcctggatc gaccgataac
      181 accaactcat gcagagtcga cagttcactc cgcttcggtg cgggatgggt agcgatgtgg
      241 cggtttattt cctgttgtgc tttcatgctg atcagtccgg ctgcgacacg ccagctgtta
      301 cttgcttagc ggtccgtctc cacgatccat tcaatgccgt acttatcccg gaacataccg
      361 aaataagttc cccagggact ttcgccgatc ggtgcctcga cagttcctcc ctgggatagt
      421 ccgtgataca agcggtccgc ctcttcccgg ctcacggcgc ggaccgagat cttggagcga
      481 ttttcctgct cattcacgcg tccgaggaag gaaggaacgt cgtttccgat cagcatacct
      541 gtgccgaccg gcaaggcgat gtggaggagc ttgtcggcgt cttcgtccgc tatcggaaca
      601 tccggtcccg ccaattcctt caggtacagg atccggctga actcaccacc gaagaccgaa
      661 cgatagaact caaaagcttc ccgtgcgttg ccgttgaagt tgatgtgggg atggagtgtg
      721 gccatatcac cgaaggtcgg gtattaccgg cgatttccat tggaccaatt gttaaagcgg
      781 tacttgcaag tattccgatg aatgactgcc catccttccg gacttaaata agaaaattcc
      841 agcaggccgt tgcaacagct gacttactcg atctcccctt cccggtccgc gacatattcg
      901 agcagatcgc ccggttgaca attcagcgcc ttgcagatcg cttccagcgt gctgaagcgg
      961 acagccttgg cttttccggt cttcaggatg gaaaggttcg acaaggtcag gtcgactttt
     1021 tccgacagct cgttcagcga catctttcgc ttcgccatca tgacatccag gtttacgacg
     1081 atcgccatgg cttatacggt tagatcgttt tcgctttgaa tgtccacgcc gcgtttgaag
     1141 atggcggcga tgatgtagat caccgcgccc atcagcacaa aggcctgact gtcctcccag
     1201 aactggtgca gcccgtcggt gatcagtccg tgttgctgaa gattcctggc aaactgccgg
     1261 gcgatataac tcaaaaaacc gaccgacaag gtgaaataac tgatccgcag gatctgactg
     1321 gaaacaaaaa ggtcgaaggg tttcgacagg ttcatggtat gcatcaggcg gatgacaatg
     1381 tagaagagga aggacttcag cagcgctatc gacaggatca gcgagtagat cccgaagaag
     1441 gatgccctgc tttgcgtcag catctcgcgc aggtccagct tctggtaaag gttcaccacg
     1501 aagtcgggtc gatataggtg aaagaagaaa ttgacgacca gcccaccggc ctcaatgctc
     1561 aagccgacga agatgaacca ggcgataatg tataacgcct tgaatacgag ggaatttgtc
     1621 ttagacatgc cgctttatga ttataaatgc tgcggcaaat ataataaata tttattgttt
     1681 gtcaataaat atatacactt attcaagaaa aatcatgtga ttcagttatt gccgctcgtc
     1741 cacttactgt tgtcaagact cctgaaacca gaaaatgaat gggataggct gatccggcac
     1801 cagatctccc tccggacttg agaaagtcga gaaatcacca ataagaggtg gactgagttc
     1861 cggccgacat gttttctggc ccatgtacga tcggctggac ctgcatatcc attgcaactt
     1921 ccgtgtttac tcgaaaacga gtttacggag gcttagttgg tcatttacgc ggaccagcag
     1981 aaaatacaga ccagcagcag gtggacggga tatttcgaac gcatgtttcc cggccgtgag
     2041 caactgcata tcattggatc gaactgccct acccgcaacg tccaatatgg tataagaaat
     2101 gttagctgat aaaggtaagt ccaggatcaa tatcgcttgt tggtaagccg ggttgggttt
     2161 aagttgaatc aaatcaacat cctccatcgt ttgcaccttg accggcaggc cacctatatt
     2221 aatgtcatcc acgtaaatac tgttaccgcc ctttgaggtg aattgaaatt taaaacaaac
     2281 ctgcgcttca ccggctgctc cattgatgtt tgcgccatcc tgccgccatt gtccgcttga
     2341 cggtatgaaa aaataggcag tgtcagttgc agtggccaga aaactcccgc tcttggaata
     2401 acgcatgttc caggtctgcc cacaatccgt actcatgaat attttcaaaa catctccgga
     2461 agcattgaca ttggcaaaag ccagtcggaa gctgaaggta ggcgatgggc aatttgaaaa
     2521 atcatagctt tccgtataga tcacatcatc ggtttcccga ttgaggctat cccgctgcag
     2581 aaaaatggaa tgactgccgg agaccgaagc gttggtggtg atctcccagg cagagcctgc
     2641 tgcattctcg acaccccagg caccggaagg gaactgcagg ctttcaaaat cctgggtgac
     2701 gggggcggtg agtgaaggga ccgcgggtaa tacgttcacc aatccgttgc gaacaatgga
     2761 agatgaaccg gacccattga aaaccgttaa gactacatcg tgaattcccg ccaccggata
     2821 ctgtacagtc gggctgggat cagtgctcga aacaatgttt cctccggtaa actgccattc
     2881 caccgaatcg acattcccgc cataagacaa attctcgaac gttatggact gcccctcgca
     2941 aatacgcatg acccggttcg tcaggtccgc gatgggcggg caggctacag gaatatatcc
     3001 atcgtttgtt ccggtcaagg ccaaattgcc ggtcgaccag agattgttcc tgtttcccgt
     3061 cggtgcattg agtgccgcct gcatgcgggt cacctgccca agcgtgaaca tctgttcgaa
     3121 acagtaatcc atgatgttct gaacattgct gatctcgccg gcaatgcagc ccggcgcatt
     3181 cagattacaa accccgccaa tgcagttggg tgtatcgaaa acctgatcgt ccaggctgca
     3241 attggatgcc aacccgggag tattcgtcgg gccccaggta tgtaacagat tgaagtagtg
     3301 accggcctca tgcgccagga ttgcgatgga tgagcttccc tgagcggtgc caatgcttcc
     3361 aacatacgga tgcgtgacga cgattccgtc taacgcagga ggcgcaccgg gcaagtaggc
     3421 atatgccgag aaactgttta acgtattttt ctccacccaa atgttcaggt agcgatcggg
     3481 gggccattgt aagatggact tcagtaagtc attcccgccg ttcgtcaggt acgtttgatg
     3541 acgggtgatc ccgtcggtac agtttccctg cggatcaagt tgcgccaggc ggaactccaa
     3601 ccggacatcc ccgatcagcg gttgaaacag gggaatgatc tgcgccgtat cggcgtttcg
     3661 tttttgatag tattcgttca tgatccggac tgcatccaca atctgctccc gtgagatatt
     3721 ctcctcaccg taattgtgca tgatgtgaaa tacgactgga atgatgaaca ggatcgtatc
     3781 cgatagcacg cgctgctgcc gatgagccgt ataagttgca gtgaattgct ccaatgcagc
     3841 acgttcatgt agcaaggcgg ggtgcttttg caacagcaat tcttccattt ctgcatggcc
     3901 gcatgcgtgt tgttgctgtg cttgtgaaac ctgacagaaa aagatgagca aggataccaa
     3961 tacgatcttc attgcgagaa aattcaggaa tcaatacttg acgactttgg tgtatagtgt
     4021 attgccctct gcctgaatcg tacacgagta tagtccggcg ggaacatccg gtaaggaaac
     4081 cggaaccgtg acgcggccgc ctggcaaccg gccatccgat acgaccttca gttcacggcc
     4141 aagtacatcg gtcagggtga tcttgcacct catgggcttg ggcaactcca ggacaacatt
     4201 aaacccgtcc tgaaacggat tgggaaatac ggaaacgccg gcgatgttct ccattgttac
     4261 tatgccggta gccaatgaat atcgtccgag gttcagccat tcatgcgcat cggaatgatt
     4321 cagggacgtg ccgggagggt tgccgccaag cgctaccaga tagaccgaat cctggtaaag
     4381 aatcggtgtc atgtagaaca ggttcctggc agttggctta tcggggccca gtttccagga
     4441 acctgaattc acatcatagg caaaggtcct gtccgtagtg gtgaagatcg aaggctcagg
     4501 cgacccaccc gcaaagacca ccagtccgga agccgggtcc agggaggtgg ccgcacctcc
     4561 gcgcgacaac atgcccgcgg gatggtcatc cgcctgcgtc caattgacct gcaatgggtc
     4621 agccacatta atttctccga cataggtcga tgccgttgcc tgaccggtca cgggattata
     4681 accgcctgca acgacgatct tattgttgac tataccggca cgggaagcat aaagcgccgg
     4741 gcctggtaat gtggttccgc tgctccaggt gtcggtgtac gcattgtaga tctgaacgag
     4801 gcttacagca cctccggtcg catcgatcag tccgcctatg atataaacga gggaatcccg
     4861 ataggtacca taagctgcag taataacggg cgtcggcatc ggactacccg tgctccaggt
     4921 atcggtggca taatcgtaga tctgggtgga accttgcgca gtgcccagtg ttcgtccgcc
     4981 gaatacgaag atctttccgt tggcggtagc cgatgacgca taggtgcatt cgacgggcat
     5041 cggtgcaatg ctcgtccagg aattgaaacc aagatcgtac tcgtaagcat ccggcagaat
     5101 ggtcaactga ccataaccgc ccaacaggaa cagacgcgaa gaagagccgg agtgaaccga
     5161 agcgacggcg ctgccaaacg tagggtcgac caggggatcg tgtatactcc agccgtagtg
     5221 ttcgaaaggc tcgagtactt tggtgatctt cgatgccgta tcg
//
LOCUS       DAOTON010000076         5254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13133_length_5254_cov_3.703982, whole genome shotgun sequence.
ACCESSION   DAOTON010000076 DAOTON010000000
VERSION     DAOTON010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13133_length_5254_cov_3.703982"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..1674)
                     /locus_tag="P5292_02480"
     CDS             complement(283..1674)
                     /locus_tag="P5292_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRS38021.1"
                     /translation="MKRFMLSFALAILGMTGASAQTTETRELPPFTGIDIRSVAKVFI
                     RQDATQEVKIVSGDRSEATETRVDGGTLNIDGAGSTIYIALPSLERIEVSGRASIMGQ
                     STFTGKALLIDIGGDGKITLDVQYESLSANISGLGSIILSGAVTNANLKISGSGKVEA
                     LDLKATNCNASISGLGKCSIDVTDNLNTNISGSGTVAYKNAPKNISQNVTGLGRVVTN
                     ESLNIKRDTTHLTFGDSEVLIISAKDSLKARKKHDVKPIWAGLDLGMNTYMTRDNSFS
                     LPAGWNGFELREEKSISVGLNLLQKDVQLGKSNFWFLTGLGVTWNNYRFSNNVSLSNK
                     DGRVSAIVDTTSEIKYQKSKLTASYLTVPVLFEYFTSKDKKKAFHFTAGAMLGLRIGS
                     HTKQKYELDGKTEKPKTYDNFYLNPFRYGARFAMGYGNFNLFADYYFSSLFKDNKGPE
                     LYPVNVGITIAGW"
     gene            complement(2129..3244)
                     /locus_tag="P5292_02485"
     CDS             complement(2129..3244)
                     /locus_tag="P5292_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38022.1"
                     /translation="MLTRSNYESFFLDYHEGNLSDALKAEVLAFLDQNPDLREEFEQF
                     AAITLEPEPVVFSEKSLLLKKPSVDQHDTEQLIALHEGDLSLLEAQQLQKRFEQEPAL
                     RKESGLIARLRIEPDQSVVFPDHNSLKKGGRVIAFRTRVYQSLSIAAAIVILLLAYVY
                     FRSEEQPMTADRHVVPADSSDTSNPEKQIMPLEKQVPPSTQYAGLPVDTSKESAPVRQ
                     LKEKQKSVAADPGSSYAQKAPAPRPIFEQPKQQIREQSPEQKQSIQPEQSPLPEPVFA
                     QQPTPAKSEPLRPAGVVNGSESLADIFSEQELAELGLREQTTKDGQLWKIVEKGARKL
                     GEATGSDVAVDHQSDPVTQSNTYALAIGKFSIRRSGR"
     gene            complement(3435..3920)
                     /locus_tag="P5292_02490"
     CDS             complement(3435..3920)
                     /locus_tag="P5292_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRS38023.1"
                     /translation="MTTKEYNDCVERHADGLYRFILKNMRDEDEARDVVQDTFEKMWR
                     NVENIDGNKAKSYLFTTGYHTMIDRIRKKSRTSELTEAHEESLQHSQQYSDLKQILHQ
                     AVNKLPEIQKAVLTLRDFEGYSYAEIGQITGLNESQVKVYIYRARLFLKQYIGSLEVV
                     V"
     gene            4057..>5254
                     /locus_tag="P5292_02495"
     CDS             4057..>5254
                     /locus_tag="P5292_02495"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRS38024.1"
                     /translation="MTRAPGNAREFDKFNIAADRHYTEAMKKNCYLILFLLFLTHHSL
                     RAQLIVNSGVLNPQQLVQTLVGPGVTISNVSFTGVLTATGSFTDTTGNLGMSNGIVLT
                     SGSPLRIPGPNNLDSAQVDNLQPGDPQLDTVYNTFTFDATILEFDFVPASDTFAFNYR
                     FGSEEYNEFVNLGVSDIFAFFVSGPGINGAENVALIPGTTTPVTIDSVNNGFTPVGTL
                     STGPCVNCQYYIDNLNGTFLQYDGYTTILTAGRKVSPCLTYHLKMVIADVGDGIYDSG
                     IFLEAGSFKSTADFRIQFNGQNAPSTVRVCPGDCAVLTAPTLPNYLWSNGETTQSITV
                     CSDGQYNVSTSTGTCSAQSNNVFVQITAGPDTPVVSIVNDTAYASVSGSFLSYQWYSG
                     GVPVSGA"
BASE COUNT         1118 a         1341 c         1518 g         1277 t
ORIGIN      
        1 ccaggttacg ggcggaggca ttcaggctga tgaagaatgc caggagggcg aggatgggtt
       61 tcatggggtg aggagttatg gggttggagt gaggggtggg tgggtaggtc gtaattagaa
      121 actagtaact agtaattagt aattggtggt tggtggttgg tgattggtcg ttggtcgttg
      181 atgaaatgac ctgaggcgtt tcttctatcc aagaactaat taccagttac cagttactag
      241 ttactagtta ccagttacca attaccaatc accaacttcg aatcaccatc ccgcgatcgt
      301 gattccgaca ttcaccggat agagctcagg acctttgttg tctttgaaga gcgaagagaa
      361 gtagtaatcc gcgaagaggt tgaagttacc atagcccatc gcgaagcggg cgccgtagcg
      421 gaacgggttc aggtagaagt tgtcgtaggt cttgggtttt tcggtcttgc cgtcgagctc
      481 atacttctgt ttggtatggg aaccgatacg gagcccaagc atggcgccgg cagtgaagtg
      541 aaacgccttc ttcttgtcct tgctggtgaa gtactcgaac agtacgggca cggtcaggta
      601 actggctgtc agtttcgact tttggtactt gatctcactg gtggtgtcaa cgatggcaga
      661 aacgcgtccg tctttgttgc tcagggaaac gttgttggag aagcggtagt tgttccaggt
      721 aacgcccagt ccggtgagga accagaagtt actcttgccg agttgcacgt ccttctgcag
      781 caggttgagt ccgaccgaga tggacttctc ttcgcgcagc tcgaaaccgt tccagccggc
      841 cggcagactg aaggagttgt cgcgggtcat gtaagtattc atacccaaat caaggcccgc
      901 ccagatcggc ttcacgtcat gcttcttgcg cgccttgagg ctgtcctttg cggagatgat
      961 gagcacttcc gaatccccaa aggtcaggtg tgtcgtatcg cgtttgatgt tgagcgactc
     1021 gttggtgact acacgcccca ggccggtgac gttctgcgaa atgtttttgg gagcgttctt
     1081 gtaggcgacc gtaccgctgc cgctgatgtt cgtgttcagg ttatcggtga catcgatgct
     1141 gcacttaccc aggccggaaa tgctggcatt gcagttggtg gctttcagat cgagcgcttc
     1201 cactttaccg gagccggaga tcttgaggtt ggcgttggtt acagcgccgc tgagtatgat
     1261 ggaacccagt ccggaaatgt tcgcgctcag cgattcgtat tgcacgtcga gtgtgatctt
     1321 accgtcgccg ccgatatcga tcaggagggc ctttcccgta aaagtcgatt gacccatgat
     1381 gctggcgcga ccggaaacct caatacgctc gagggagggc agtgcgatgt agatcgtcga
     1441 accggcgccg tcgatgttca gggtaccacc atcgacgcgg gtctcggtag cttcgctacg
     1501 gtcaccgctg acgatcttga cttcctgggt tgcatcctga cggatgaaga ccttggccac
     1561 ggagcggata tcgattccgg tgaagggagg caactcccgg gtttccgtgg tctgtgcgct
     1621 ggcacccgtc atcccgagga tagccagtgc gaaagagagc atgaagcgtt tcatatgtgt
     1681 aggatttagt ttgtttttcg cttgtttatg aataggacgg ttgagtgggg cggttgttac
     1741 aggatgagaa aaaaaagtgc gagaccggga gggagggacg tgggacgagg gaggtgggac
     1801 gagggacgag ggacgtggga cgagggacga gggggtggaa actgggaatt agtaattagt
     1861 aatttgtaat tagtaattgg ttattggaga ttagggatct ttatgaagcc tggtcacggc
     1921 ttggtcaatc gataaaaagg gttgcgacgg aagattcgtt gtcacaataa ctgaaaacac
     1981 gaaacctgcc tgcctgcctg cctgcctgcg ctaaagcttc ggcaggctag cacgaaactc
     2041 aaaacacgga acacgcctgc ctgccgaagc tttagcgtag gcagggaact cggaacattt
     2101 tcacctcagt tccgttgctc gtacctactt accggccgga acgacggatg gagaacttgc
     2161 cgatagcgag tgcgtaagtg ttggattggg tgacgggatc cgactgatgg tcgacggcta
     2221 cgtcggagcc ggtcgcttct cccaacttcc gggcgccttt ttcgacgatc ttccagagtt
     2281 gtccgtcttt ggtagtctgt tcacgcagac cgagctcggc gagttcctgc tcggagaaaa
     2341 tatcggccag ggattcactg ccgttgacga caccggccgg acgtaagggt tcggatttgg
     2401 cgggagttgg ttgctgcgcg aacaccggct ccggtagtgg cgattgctca ggctgaatgc
     2461 tctgcttttg ttccggcgac tgctcccgga tctgctgctt cggttgttcg aagatcggac
     2521 gcggagccgg cgctttttgc gcgtaagacg agcccggatc cgctgccacc gacttctgct
     2581 tctctttgag ctgacgaaca ggtgccgatt ctttggatgt atccaccggc aaaccggcgt
     2641 actgcgtcga aggtggtact tgcttttcca gcggcatgat ctgcttttcc ggattggaag
     2701 tatcggaaga atctgcagga acgacgtgac gatcggccgt catcggttgt tcttcggaac
     2761 ggaagtagac atacgccagg agtaagatca caatggcagc agcgatggaa agcgactgat
     2821 acacccgggt ccggaaggca atgacgcgtc ctcccttctt taggctattg tggtcaggga
     2881 acaccaccga ctgatcgggc tcgatgcgaa ggcgggcgat caacccggac tccttgcgaa
     2941 gggccggctc ctgctcgaaa cgcttttgaa gttgctgcgc ttcaagaagg gacaaatcac
     3001 cttcgtgcag cgcgatcaac tgctcggtat cgtgctggtc gaccgatggt tttttcagca
     3061 acaaagattt ctccgaaaaa acgaccggct ccggctccaa cgtaatagca gcaaactgct
     3121 caaactcctc ccgcaggtcc ggattctgat ccagaaaagc caacacctcc gccttcagcg
     3181 catcgctgag gttcccctcg tggtagtcga ggaagaagga ttcgtagttg gatcgggtta
     3241 acatgaggga cgaggggcga gggacgaggg ttggaggttt agggcttcct gcctacgcta
     3301 aagctacggc aggcaagcgg gttccgggtt tcgagtttcg agtttcgagt ttcgggtttc
     3361 gagttgaaaa aggactattt actatttact gttcactatt cactgttcac tattcactgt
     3421 tcactgttca ctcctcaaac aaccacctcc aacgacccga tgtactgctt gaggaacaat
     3481 cgggcgcggt agatgtagac tttgacttgt gattcgttga ggccggtgat ctggccgatt
     3541 tcggcgtagc tgtaaccttc gaagtcgcgc agcgtcagca cggctttttg gatctccggg
     3601 agtttgttga ccgcctggtg gaggatctgt ttgagatcgg aatactgttg ggaatgctgc
     3661 agactttctt cgtgcgcttc ggtgagttcg ctggtgcgcg actttttccg gatgcggtcg
     3721 atcatggtgt gataacccgt ggtgaacagg taactcttgg ccttgtttcc gtcgatgttt
     3781 tccacgttgc gccacatttt ctcgaaggta tcctgcacca cgtcacgtgc ctcgtcttca
     3841 tcccgcatgt tcttcaggat gaaacggtac agaccgtctg cgtgccgctc gacacaatcg
     3901 ttgtattctt ttgtggtcat ggttcccggt gggggtcgga aatcagtgat ctaattatgg
     3961 gacgggctcc cgggtcattt gttacaggcc aggtattcaa tttatattat tgattatcat
     4021 atacttatca ataatttcag gattatgccc gatgccgtga cgagggcccc gggaaatgca
     4081 cgggaattcg ataaattcaa catcgctgcc gaccggcatt acaccgaagc tatgaaaaag
     4141 aactgctacc tgatcctgtt cctcttgttt ttaacgcatc acagcttacg cgctcagctc
     4201 atcgtcaaca gcggcgtgct gaacccgcag caactggtac agaccctggt gggtccgggc
     4261 gtgacgatca gcaatgtttc gttcaccggc gtgctcacgg ccacgggctc gttcaccgac
     4321 acgaccggaa acctgggcat gagcaacggc atcgtactca cgagtggtag tccgcttcgc
     4381 atcccgggac cgaacaacct cgacagcgcg caggtcgaca atctgcagcc gggcgacccc
     4441 caactcgaca cggtgtacaa cacgttcacc ttcgacgcga ccattttgga attcgatttc
     4501 gttccggcat ccgacacctt cgctttcaac taccgattcg gttccgaaga gtacaacgaa
     4561 ttcgtcaacc tcggggtgag cgacatcttc gcgttcttcg tcagcggtcc gggtatcaac
     4621 ggagcggaga acgtagccct gatccccggc accaccacgc cggtgacgat tgacagcgtc
     4681 aacaacggtt tcacgcccgt cggaacactc tcaaccggcc cgtgtgtgaa ttgtcaatac
     4741 tacattgaca acctaaacgg cacctttctg cagtacgatg gttatacgac catcctcacg
     4801 gccggccgga aagtctcgcc ctgtctcacc taccacctga agatggtcat cgccgacgta
     4861 ggcgacggca tctatgattc gggcattttc ctggaagccg gcagtttcaa gtccaccgcc
     4921 gatttccgca tccagttcaa cggacaaaac gctccttcta ccgtgcgtgt ttgtccgggt
     4981 gattgcgccg tactcaccgc ccctaccctg cccaattact tgtggagcaa cggagaaacg
     5041 acccagtcga ttaccgtgtg cagcgacggg cagtacaacg tgagtacttc caccggcact
     5101 tgttcggcac aaagcaacaa cgtcttcgta caaatcaccg cgggccccga cacgccggtc
     5161 gtaagcatcg tgaacgatac ggcttatgcc agcgtgagcg gttccttcct ttcctaccag
     5221 tggtacagcg gcggtgttcc cgtgagtgga gcta
//
LOCUS       DAOTON010000077         5248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13148_length_5248_cov_3.010013, whole genome shotgun sequence.
ACCESSION   DAOTON010000077 DAOTON010000000
VERSION     DAOTON010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13148_length_5248_cov_3.010013"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /gene="asnB"
                     /locus_tag="P5292_02500"
     CDS             complement(<1..892)
                     /gene="asnB"
                     /locus_tag="P5292_02500"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRS38025.1"
                     /translation="MCGINGFWNLHGRRLDHAPELMGRMNQALQHRGPDDEGYWGTET
                     EGVHLGHRRLSIIDLSPAGHQPMLSPSGNVIVFNGEIYNYKELREQFFRGERLRSDSD
                     TEILLLLYERFGERCLDHLNGMFAFAIWDPRKEELFLARDRAGKKPIYYTLQEGVFAF
                     SSELRSLFELPFVKRELDESALYHFLTYNLLPPPLTMFKGIHKFHPAHWMKVKRNGIH
                     EYQPYWEVGYQHTGARDERELSAEVLEGLRKAVSYRMVADVPVGAFLSGGVDSSAIVA
                     LMSGMTTVPVKTYSIGFSGQP"
     gene            complement(980..1990)
                     /locus_tag="P5292_02505"
     CDS             complement(980..1990)
                     /locus_tag="P5292_02505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS38026.1"
                     /translation="MNILWQSKTATVAEAFRVGPDQIPMNGGNAYDVQAAIALKNHFS
                     IAIDPVTIRGKENVLAYWWKMSRHKAKADLLILEPFPIVYGRRLPGQRSIAMIHHIDP
                     VIRSSSRYHRWYFDRLIRRTKSCDCVVTVSRYWQDYFITQGCNKVEVIYNAFDPEPYR
                     TPGFDARAFRVRHGLPEDKPLVYIGNAIREKGVYEVSEALKDQPYHLVMTGAANRAGD
                     LPVQYLKLNRQDYRGLLRASDVVIAYSRMIEGWNRIAHEALLSGTPVVGSGTGGMHEL
                     LENAGQPEVKNPEELVTAVEAVLRDRQTYSAKGYDYIRQFDLAYFERRWKAVVDQVMQ
                     GG"
     gene            complement(2034..3035)
                     /locus_tag="P5292_02510"
     CDS             complement(2034..3035)
                     /locus_tag="P5292_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38027.1"
                     /translation="MRGPSFQYSDFVRPATREDYARAIDNRIEWLRRQPGLVTVYSIG
                     SVGHPGISDIDLVAVFEDDIAVAEHPLDGLDPMSRYLFVHTLYGARRSDFLAAQRFSF
                     YQNYHLLSGENLLAGSQDLPPGHRSTIERQIALEFMLRMYISLFLQREYGLLRIRTLL
                     LHGKGLLNDARSLGIPQADWIGMLEEVMMMRDQWFTRSEAENNRAVDDWFRRFDPAYG
                     AFLKTYLQAEPFEVPATSGFNYAKNIQVRSGEVLRASRHGFRIPFGNALLRRKYFNLQ
                     NRLNRFVFEVPFISSVNNPLVREYYAFSTGLRDRTRQNLPGFFALTSAFQVGHANSP"
     gene            complement(3032..4039)
                     /locus_tag="P5292_02515"
     CDS             complement(3032..4039)
                     /locus_tag="P5292_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38028.1"
                     /translation="MKRSEFRARLNRYVNGAIFSTSDQVRSLLSDTWSFRAHAAQICE
                     DYRVLEPALPTLPAEEVVIPPYKYRDVRIEDLRGTLEKEFRRDIDGIVLHGSFGSGEP
                     VAYSDFDGLILLNDEVFQDAPRLADLAARLHRLRSPMLQIDPLQHHGWFVLTSSDLRQ
                     YPESFLPVEVLKNACSIYGYKDMRLSISRLEHDPLDQGFRRMADTLSGKIRNGRVPAN
                     FYQAKVFMSEVMLLPALYVQARDQKGIFKRESFAAARADFSPSVWSIMDEYSAYRANW
                     NFQPRGFDKWMLSKSSWLWLHWRKSRGTPLSREVKALFEQGKLQALETLITEMRRRIG
                     K"
     gene            complement(4195..>5248)
                     /locus_tag="P5292_02520"
     CDS             complement(4195..>5248)
                     /locus_tag="P5292_02520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS38029.1"
                     /translation="YPYTYMRDNIRRAEIPKMYGTRFSVRERMHWTPLREHSPSWCRI
                     GWIMFAFSMTALRLRFFSGKRKQTLVFSRDPNLLLPILLLMKWLGGRPAIRTVFLLAE
                     MKDRKLYKWVAKNSDFLLAGVSATKDAVRKLVDVPEDRFMLALAPVPAYPNDCTKEEA
                     RRRIGYSEKAPLVVYTGKLGLSNREVRHILEAAGLEPNYRFLFTGGRASTVEAIKAWC
                     AERNIRNVILTGFLNDSTAIRDYQLAADVLVSYYTSEDHMVEFNYPQKINEYLSTGNP
                     VVTPDFAATRDVINDRNVIFVEPDDSKALLNGIRKAIEQPEWAAGIAAQAKKDVAPLS
                     FDERTGAWLRFVLKRD"
BASE COUNT         1247 a         1477 c         1373 g         1151 t
ORIGIN      
        1 cgggctgacc ggaaaatccg atggaatacg ttttgaccgg cacagttgtc atccccgaca
       61 tcaacgcgac aatggcgctg gaatctacac caccactgag gaatgctcct accggcacat
      121 cggcaaccat gcgataagac accgctttgc gcagtccttc caatacttcc gcggaaagct
      181 cccgctcgtc gcgtgcgccg gtatgctggt atccgacctc ccaatacggt tggtactcgt
      241 gaatgccatt ccgtttcacc ttcatccaat gcgccgggtg aaacttgtga atgcctttga
      301 acatcgtcaa aggaggcgga agcagattat aggtcaggaa atgatacagt gccgactcgt
      361 cgagttcccg tttcacaaaa ggcagttcga agagagagcg caactcggaa gagaaggcaa
      421 agacgccctc ctgcaaggtg taatagatcg gcttcttacc ggcacggtca cgcgccagga
      481 agagctcttc ctttcgcgga tcccagatcg cgaacgcgaa catcccgttg agatgatcca
      541 ggcaacgctc gccgaagcgc tcgtagagta ataacaggat ctcggtatcg ctatcggacc
      601 tcaaccgctc cccgcggaaa aactgctccc ggagttcctt gtaattatag atctccccgt
      661 tgaagacgat cacattcccg gaagggctga gcatcggctg atgcccggcg ggcgagaggt
      721 cgatgatgct caggcgacga tggccaaggt gtacaccttc cgtttcggtc ccccagtagc
      781 cttcatcatc cggcccacgg tgttgcaaag cctgattcat gcgacccatc agctcgggag
      841 catgatccag gcgcctaccg tgaagattcc aaaaaccgtt gatgccgcac atgaggacag
      901 atacgctgct gtcgtaaaaa cgcagctgcg acgattatag caagataact attacggaga
      961 aaaacggttt ccgttccatt tatccgcctt gcattacctg gtcgaccacg gctttccagc
     1021 gtcgctcaaa ataggccagg tcaaactgac ggatatagtc gtatccctta gcggaatacg
     1081 tctgccgatc cctgagtacc gcttccacgg cggtcaccaa ctcttcggga ttcttaactt
     1141 cgggttgccc ggcgttttcc aggagttcat gcatgccccc ggtgccgctt cctacgaccg
     1201 gagtaccgct cagcaaggct tcgtgggcga tacggttcca gccctcgatc atgcgggaat
     1261 atgcgatcac cacatcggat gctcgcagca agccacgata atcctgacgg ttgagcttca
     1321 ggtactgcac gggaaggtct ccggcacggt tggcagcacc ggtcatgacc aggtggtagg
     1381 gttgatcctt caaggcttcg gatacctcgt aaacgccctt ttcccgaatg gcattaccga
     1441 tataaaccag tggtttgtct tccggcagtc catgtcggac ccgaaaggca cgcgcatcga
     1501 atccgggagt acgatatggt tcaggatcaa aggcgttgta aatgacctct accttattac
     1561 aaccctgtgt gatgaaatag tcctgccaat agcgggaaac cgtgaccacg caatcgcagg
     1621 atttcgtgcg gcggatcagg cggtcgaaat accaacggtg gtagcgactg gagcttcgga
     1681 tcaccggatc gatatgatgg atcatcgcga tggaacgctg ccctggcaaa cgtcggccgt
     1741 aaacgatcgg gaacggctcc aggatgagca gatcggcctt tgccttgtgg cgcgacatct
     1801 tccaccaata cgccagcaca ttctcctttc cccgaatggt aacaggatcg atcgcaatcg
     1861 aaaagtggtt cttcaacgca atagcagcct gaacatcgta ggcatttcca ccgttcatgg
     1921 gaatctgatc cgggccgacc cggaatgcct cggctacggt agccgtctta ctttgccaaa
     1981 ggatgttcat ccgtcttaag cgaatacggc agaatactcg ttacggttcc agatcatggt
     2041 gaattcgcat ggccaacctg aaaggcactg gtgagggcga agaaacccgg caggttttgc
     2101 cgtgtccggt ctctcaatcc ggttgaaaag gcataatact cgcgcaccag cgggttgttg
     2161 acactactga tgaacggaac ttcgaacacg aacctgttca ggcggttttg aaggttgaag
     2221 tacttccgcc tgaggagcgc gttaccgaac gggatacgaa aaccgtgtcg gcttgcacgc
     2281 aagacctcgc cggaccgaac ctggatattc tttgcgtagt tgaacccgga agttgccggc
     2341 acttcaaacg gctccgcttg caggtacgtc ttcaggaaag ctccataagc aggatcaaaa
     2401 cggcgaaacc aatcgtccac cgcccggttg ttttcggctt cacttctcgt gaaccattgg
     2461 tcacgcatca tcatcacttc ctcgagcatc ccgatccagt ccgcctgtgg aataccgagt
     2521 gaacgggcat cgttcagtaa acctttcccg tgcaacaaga gcgtccggat acgaagcagc
     2581 ccgtattccc gttggagaaa caagctgatg tacatccgaa gcatgaattc aagggcgatc
     2641 tgtcgttcga tcgtggagcg atgaccggga ggtaaatcct gactgccagc cagcaggttt
     2701 tcgcccgaaa gcagatgata attctgatag aacgaaaaac gctgtgcagc caggaagtcg
     2761 gatcgccttg ctccgtagag ggtatgtacg aacagataac gcgacatcgg atcaagacca
     2821 tccaaaggat gttcagcaac cgcgatatcg tcctcaaaga cggctacgag atctatatcc
     2881 gaaatcccgg gatgcccgac actgccgatc gaatagacag tgaccaaacc gggctgtctt
     2941 cgcaaccatt cgatgcggtt atcgatagcg cgcgcatagt cttcccgtgt agcgggcctg
     3001 acaaagtcgg aatattgaaa cgaaggccct ctcattttcc gattcgacga cgcatctccg
     3061 tgatcagcgt ctcgagggct tgcaattttc cctgctcaaa caatgctttc acttcccgac
     3121 tcaggggcgt tccacgggac tttctccagt gcaaccagag ccaggagctt tttgacagca
     3181 tccatttatc gaatcctctc ggctgaaaat tccaattggc ccggtaggcc gagtattcat
     3241 ccatgataga ccagaccgaa gggctgaaat cggcacgggc agcggcaaag ctctcccgct
     3301 tgaagattcc tttttggtcg cgggcttgca catacaaggc tggcaatagc atcacttcgc
     3361 tcatgaagac cttggcctgg tagaaattcg cagggacgcg cccattgcgg atctttccag
     3421 acaaagtatc cgccattctc ctgaaaccct gatccagagg atcgtgctcc aacctggaga
     3481 tagaaaggcg catatccttg tatccgtaga tgctgcaagc attcttaagc acttcaacag
     3541 gcaagaaaga ttccggatac tgacggagat ccgaggaagt cagtacaaac catccatggt
     3601 gctgcaaggg gtcgatttgc aacataggtg aacgcaatcg gtgcagtcgg gcagccagat
     3661 cggcaagcct gggcgcgtct tgaaatactt cgtcgttcag caggatcagt ccgtcaaaat
     3721 cactgtacgc aaccggctcc ccgctaccga aactgccatg caggacgatt ccgtcgatgt
     3781 cgcgtcgaaa ctctttctcc agggtgccgc ggaggtcctc tatccgaaca tcccggtatt
     3841 tgtagggagg aattacaacc tcctctgcag gcagggtggg tagtgcgggt tccagaacgc
     3901 gatagtcttc gcaaatctgg gccgcatgcg cccgaaaact ccaggtgtcg gaaagcaacg
     3961 aacgcacctg atcggaagtg ctgaagatcg cgccattcac atagcggttc agcctggccc
     4021 ggaattccga tcgtttcatg ctcataacag gacgctgtaa tctgccactt tcattcgatt
     4081 gtttcaggct ctgctcatcg gaactccaaa acttccgcaa tttacggtac agcgggcaat
     4141 ccgccttgcg aatcatgcag agttatccct ccatcaggag cacttgtcag ggactcaatc
     4201 ccttttcagc acaaagcgca gccaggcacc ggtccgttcg tcgaaagaga gcggcgcaac
     4261 atccttttta gcctgagctg cgatcccggc cgcccattcc ggttgttcga tggctttccg
     4321 gattccgttg agcagggctt tgctgtcgtc cggttctacg aaaatcacgt tccgatcgtt
     4381 gatcacatca cgtgtagcgg cgaagtcggg ggtcacgacc gggtttccgg tcgagaggta
     4441 ttcgttgatc ttttgtgggt agttgaattc caccatatgg tcctcggagg tataataaga
     4501 aaccaacaca tcggccgcga gttgataatc gcggatagca gtggaatcgt tcagaaaccc
     4561 ggtcagtatt acgttgcgaa tgtttcgctc cgcacaccag gccttgatcg cttcaaccgt
     4621 gctggcacga ccgccggtaa acaggaaccg gtagttcggc tccaacccag cggcttcgag
     4681 gatatgccgc acttcccggt tactcaatcc taacttgccg gtatacacca ccaatggcgc
     4741 tttttccgaa tagccgatac gccgacgggc ctcctctttg gtacaatcat tcggatatgc
     4801 gggaaccggc gcaagcgcga gcatgaaccg gtcttctggc acgtcaacca gtttccgaac
     4861 cgcgtctttc gtcgctgaaa ctcctgccag gagaaaatcg ctgttcttcg ccacccactt
     4921 gtacaactta cggtccttca tttccgccag cagaaagacg gttctgatcg cgggtctgcc
     4981 gcccagccat ttcatcagca gcaggatcgg cagcaggaga ttggggtcgc gtgaaaatac
     5041 cagcgtttgt tttcgcttac cggaaaagaa ccgcaggcgc aaggcggtca tgctaaaggc
     5101 gaacatgatc cagccgatcc tacaccagga ggggctgtgt tcgcgcaaag gcgtccagtg
     5161 catgcgttca cgaacggaaa accgcgtgcc atacatcttc gggatctcgg cacgccggat
     5221 gttgtcccgc atatatgtgt acggatag
//
LOCUS       DAOTON010000078         5208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13276_length_5208_cov_3.785950, whole genome shotgun sequence.
ACCESSION   DAOTON010000078 DAOTON010000000
VERSION     DAOTON010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13276_length_5208_cov_3.785950"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..471)
                     /locus_tag="P5292_02525"
     CDS             complement(1..471)
                     /locus_tag="P5292_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS38030.1"
                     /translation="MDLQNQRIVIVDDDPNFAALLRYELSMNGYLRVEYFQDPDAFLQ
                     TLADPPALVLLDYQFDDRTGLELMREVRTSHPDVHVMMLTAYGDVSNAVASLKEGAFD
                     FIEKGQEAFPKIMESLTRLQQLELAIHGTKANGKRSVFGLFSWVPLLGVLGGSQ"
     gene            complement(1133..4312)
                     /locus_tag="P5292_02530"
     CDS             complement(1133..4312)
                     /locus_tag="P5292_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRS38031.1"
                     /translation="MPHNFLKYLVQEMERTPEANPLAPEVLKQYFLEHLLKNPEAVAE
                     QVATLMTSTSRRMEDYKLHLNEQNRELEENAKMLQASNLRLEQEAESQRKSLRNLRVT
                     ADFLAQYTDGSGLTEADTLESLSERVAELIRQREQDRQLLEESQDRYSSLVEQMRQVV
                     FQTDYEGRIIFLNSAWVHTTGYSVEESIGKDILGFLQPEDAAIAQQRYEQMKLSSGNA
                     DSIQVRFVHKDGQERYIEVRSRLQLTRDGHPNGVSGTFHDVTDSVMSARRLQELKDFY
                     ESILNGLPLGIAVMDPEQRYLFANPAAVADPEARQWILGQTPEAYCQKYGYDASLAEQ
                     RKFTFQRAVRTRAAVENEEVFLRSDNTPGHYLRNTTPFFDEQGVLKYMISYGLDLTER
                     KHTENQLQSTASRLTTLIGNMNAGIVVEDEHRKIVLVNDHLCELFQVTEPAESLSGRE
                     SSELAVFSSASFRESGRFLIRIEEIVRQRQPVYNEVLEGADGRVYERDYIPIRGERQE
                     FYGHLWQYRDITERVRMDLQLLEAKETAEESLRAKELFLANMSHEIRTPMNAILGMSN
                     LLLKTEVTPKQNQYLEAIHNSSRNLLVIINDILDFSKIEAGRLELDKVGFRMKKLLLS
                     TLDPFHYTAAEKSIGLNIDIQGLDEVVLLGDPVRFSQVVINLVTNAIKFTESGAVHVH
                     VRKRSEDASRVTIECSVTDTGIGIPQDKLDTIFESFTQADVGVTRKFGGTGLGLSISQ
                     KLTEIMGGRIRVESRVNLGTTFTIEITFERGSSVDIEEEKPALIPAVELGTLRILLAE
                     DHEYNRMYATSLLQEWGYEIDVAVNGVEAVQRLREAKYDLVLMDVQMPELDGLGATRV
                     IRTELPESAANVPIIALTANAIKGDNERCLQAGMDDYVAKPFDPQQLHNKIVALVQKR
                     RGISARPIETMQKPIPAASVDLSDTEPLVDLSFLRKMSRGNQVFVMNMINLFFETSQP
                     LIEQVRLHHKNGEMEKVRKSLHKMKPSVDSMGMRSLKQLMSRIENAIDKQQHADKIDD
                     WLYETYQLYTHCEEALRSEQARLEQEAVS"
     gene            complement(4326..>5208)
                     /locus_tag="P5292_02535"
     CDS             complement(4326..>5208)
                     /locus_tag="P5292_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRS38032.1"
                     /translation="KAASSQITVSVTGETGTGKELVAKAIHYHSPRKEKPFVAVNVAA
                     IPRELLESELFGHEKGAFTGAIASRAGKFEEADGGTLFLDEIGELDLALQAKLLRVLQ
                     EREVVRIGGNKVLKVDVRVVIATHRNLQEEVRKGNFREDLYYRLLGLPIHLPPLRERG
                     NDILVIARWLLTQFCKDNRMEALSFSPEAQDKLLRYYYPGNVRELKAMVELAAVLSTG
                     NTITADDIRLENTSNMDAFAFEETTLEEYTMRIIRHFLDKYNNNVVLVARKLGVGKST
                     IYRYIKENKLQTIRIEA"
BASE COUNT         1049 a         1494 c         1431 g         1234 t
ORIGIN      
        1 tcactgtgaa ccacccagga cgccgagcaa cggcacccaa ctgaacaacc cgaatacgct
       61 gcgcttcccg ttggccttgg tgccatgaat ggctaactcc agttgctgga gtcgcgtcag
      121 gctttccatg atcttcggaa aggcttcctg acccttctcg atgaaatcaa acgccccttc
      181 tttcagggag gccacggcat tcgacacatc accataggcg gtgagcatca tgacgtgtac
      241 atcgggatgg gaagtgcgga cctcgcgcat caactcaaga ccggtacggt cgtcgaattg
      301 atagtcgagc aacaccagag cgggaggatc cgccagtgtc tgtaaaaatg cgtcggggtc
      361 ctggaagtac tctacccgga ggtaaccgtt catcgacagt tcgtaccgga gcagggctgc
      421 gaaattcgga tcgtcatcga cgatcactat gcgttggttt tgcaaatcca tgggcgtgag
      481 atttgatcgc gcgccccttg ccaaacccga gccagcacgg aaaaaggccc tatttggtca
      541 aatttcaaat attttaagca cctctgtttc catttctggg aatgttattc cgggatggag
      601 gcgggtgcgg ctggcagtag gcggttggcg gcaggcggtt tgtagaagca acagtgggtt
      661 tcaaatgata aatagtgaat cctgcccgcc ggagctttag tgtaggcggg agtgaatagt
      721 gaatcctgtc cgccggagct ttagcgtagg cgggagtaaa cagcgaatag ccaatagcga
      781 acagcgaata gttttttgtg aattttccct gccgaaactt tagcgtaggc aggagtgaat
      841 agtgaatagt gaatagtgaa cagtgaatag ctgttcgttc ctcgcatccc gctccttttc
      901 ctcggtctcg gtctcgtaac tcgtaactta tcctcagtct cgttactcgt aacctccgcc
      961 aggtttcgtc ccgccgttcc cagctagccc cgggtgatct cgtcacgctt cgaacggcca
     1021 tcgttgttgt ggcgttgtgg tgacgagaga acacgggacc gccgctgcca cgaagcatgg
     1081 cgttgtgctc caaataaata atcggaaaat ctaacgggct aagccgcccg gttcaggaga
     1141 ccgcttcctg ttcgaggcgt gcctgctccg aacgcagggc ttcttcgcag tgcgtgtaca
     1201 gttgatacgt ctcgtacaac cagtcgtcga tcttgtcggc gtgctgctgc ttgtcgatcg
     1261 cgttctcgat ccgggacatg agttgcttga gcgaacgcat gcccatcgag tcgaccgatg
     1321 gcttcatctt gtgcagcgac tttcgcactt tttccatttc gccgttcttg tgatggaggc
     1381 gcacctgctc gatgagcggc tgcgaggtct cgaagaacag gttgatcatg ttcatcacga
     1441 acacctggtt gccgcgcgac atcttgcgca ggaatgaaag atcgaccagg ggttccgtat
     1501 ccgagagatc gaccgaggcg gcaggaatgg gcttttgcat ggtttcgatg ggccgggcgg
     1561 aaatgccgcg gcgcttctgt accagggcga cgatcttgtt gtgcaattgc tgcggatcga
     1621 acggcttggc aacataatca tccatgccgg cctgcaggca gcgttcgtta tcgcctttga
     1681 tggcgttcgc cgtgagggca atgatcggca cgttggccgc cgactcaggc agttccgtcc
     1741 gaatgacacg ggtagcgccc agtccatcga gctcgggcat ctgcacgtcc atcaacacca
     1801 ggtcgtactt cgcttcgcgc agtcgctgga cagcctccac accgtttacg gccacatcga
     1861 tctcatatcc ccactcctgt aaaagcgatg tcgcgtacat gcggttgtac tcgtggtcct
     1921 cggccagcaa gatgcgcaag gtgcccagtt ccaccgcggg aatcagcgcc ggtttctcct
     1981 cttcgatatc gacactgctg ccccgctcaa aagtgatctc gatggtaaac gtcgttccga
     2041 gattgacgcg gctttccacg cggatgcgtc cgcccatgat ctccgtgagt ttctgcgaga
     2101 tcgacaagcc gaggccggta cccccgaact tccgcgtgac ccctacatcg gcctgcgtga
     2161 acgattcgaa gatcgtatcg agcttgtcct gcgggatacc gataccggta tccgtgacgg
     2221 aacactcgat cgtcacgcga ctggcgtctt ccgagcgttt gcggacgtgc acatggactg
     2281 ctccgctttc ggtgaacttg atcgcgttcg tcacgaggtt gatcaccact tgcgaaaaac
     2341 gcacggggtc gccgagcagc acgacttcgt cgaggccctg tatgtcgatg ttcagaccga
     2401 tggatttctc cgcggcggta taatggaacg gatcaagcgt cgacaagagc aacttcttca
     2461 tgcggaagcc gaccttatcc aactccaacc gtcccgcctc gatcttggaa aagtcgagga
     2521 tgtcgttgat gatcaccaac aggttccggg atgaattgtg gatcgcctca aggtattggt
     2581 tctgcttcgg cgtcacttcg gtcttgagca acaggttgct catgccgagg atggcgttca
     2641 tcggcgtacg gatctcgtgg ctcatgttag ccaggaacaa ctccttcgcc cgcaaagatt
     2701 cttcagccgt ctccttcgct tccaacaact gcaggtccat ccgcacccgt tcggtgatgt
     2761 cgcggtactg ccaaaggtgt ccgtagaact cctggcgttc tccgcggatc gggatataat
     2821 cgcgctcata caccctgcca tcggcacctt ccaggacttc attgtacacc ggctgtcgtt
     2881 gccggacgat ctcctcgatt cgaatcagga aacgacccga ttcgcggaag gacgcgctgc
     2941 tgaaaaccgc cagctccgag ctctcgcgcc cgctgagcga ttccgccggt tccgtcacct
     3001 ggaacagttc gcacaaatgg tcgttcacga gtacgatctt ccggtgttcg tcttccacca
     3061 caatacccgc gttcatgttc ccgatgagcg tcgtcagacg ggaagcggtc gactgcaact
     3121 ggttctccgt atgcttccgc tcggtgaggt ccagtccgta gctgatcatg tatttcaaca
     3181 caccctgctc gtcaaagaag ggcgtcgtat tcctgaggta atggccgggg gtattatctg
     3241 acctgagaaa gacctcttca ttctccactg ctgcgcgcgt ccggacggca cgctgaaatg
     3301 tgaactttcg ttgttcagcc aggctcgcat cgtaaccgta cttctgacaa tacgcttcgg
     3361 gcgtctgacc gagaatccac tgtcgtgcct cgggatccgc tacggcggcg ggattggcga
     3421 agagataacg ctgctcagga tccatcacgg cgatgccgag tggcagtccg ttcaggatgg
     3481 attcgtaaaa gtctttcaac tcctgcagac gacgggctga catcacacta tcggtcacat
     3541 catggaacgt tccgctcacc ccattgggat gaccatcgcg cgtaagctgt agccggctcc
     3601 ggacttcgat ataacgctcc tgcccgtctt tgtgaacaaa gcggacctga atggaatcag
     3661 cgtttcccga cgatagcttc atctgttcgt aacgctgctg tgcgatcgct gcgtcttccg
     3721 gctgcaggaa tccgagaatg tccttgccga ttgactcctc gactgaatag ccggtggtat
     3781 gtacccaggc ggaattgagg aagatgatcc gtccttcgta atcggtctga aacacaacct
     3841 gccgcatctg ctccacgagc gagctgtaac ggtcctggct ttcttccagt agctgacggt
     3901 cctgttcacg ttgccggatc agttccgcta cacgttccga aagtgattcc agcgtatccg
     3961 cttccgtcag tccggaaccg tccgtgtact gcgccaggaa gtcggcggtg acgcgcagat
     4021 tgcggagtga tttccgctga gattccgctt cctgttccag gcgcaggttg gacgcctgca
     4081 acatcttagc gttctcctcg agctcacggt tctgttcgtt caggtggagt ttgtaatcct
     4141 ccatccgccg actcgtactg gtcatgagcg tggcgacctg ctcggctacg gcctccggat
     4201 tcttgagcag gtgttcaaga aaatactgtt tcagcacttc aggcgcgagc ggattcgctt
     4261 ccggcgtccg ctccatctcc tgaacgagat acttcaaaaa attatggggc atacgaatgg
     4321 tcgaatcagg cttcgatgcg gatcgtctgg agtttgtttt ccttgatata gcggtagatc
     4381 gtcgacttgc ctacacccag tttgcgggcg accagcacga cgttgttgtt atacttgtcg
     4441 aggaagtgac ggatgatgcg catcgtatat tcctccaggg tggtctcttc gaaggcgaac
     4501 gcgtccatgt tgctcgtgtt ctccagccgg atatcgtccg ctgtgatcgt attgccggtc
     4561 gataacacgg ccgccagttc gaccatggct ttcaactcgc gcacgttacc cgggtaatag
     4621 taacgcaaca acttatcctg cgcttccgga gaaaaactca gggcctccat ccggttgtcc
     4681 ttgcagaact gggtcaacag ccagcgggcg atcaccagga tgtcgtttcc acgctcgcgc
     4741 aagggcggca ggtggatcgg taatccaagc agacgatagt ataagtcctc gcggaaattg
     4801 cccttccgga cttcctcctg caggttacgg tgcgtggcga tgacaacacg tacatcgact
     4861 ttcaacactt tgttccctcc gatgcgcacg acctcgcgct cttgcaacac gcgcaacaac
     4921 tttgcctgaa gggcaaggtc tagctcgccg atctcgtcga ggaacaaggt gccgccgtca
     4981 gcctcttcga acttaccggc acgcgaagcg atcgccccgg tgaaggcgcc cttctcatgg
     5041 ccgaacagtt cgctttccag caactcgcgc gggatcgccg cgacgttcac cgcgacgaat
     5101 ggcttctctt ttctgggtga atggtagtgg atggcttttg caaccagctc cttcccggtt
     5161 ccggtttcac cggtgactga taccgtgatc tgactcgacg ctgccttt
//
LOCUS       DAOTON010000079         5199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13298_length_5199_cov_3.411936, whole genome shotgun sequence.
ACCESSION   DAOTON010000079 DAOTON010000000
VERSION     DAOTON010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13298_length_5199_cov_3.411936"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="P5292_02540"
     CDS             <1..437
                     /locus_tag="P5292_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38033.1"
                     /translation="PLSFESDESEQRHDFGIMMTQSPKSKPQALSMLQTYVLDEIDEC
                     YFDIAIEELSGYDELVTDSDFDLVDAMLCGIACDIDMKLAKVQKPTDKPVMEQQWVYN
                     QQSGTYQLKQVAVYPSSKDDEQDKEQRFKDPFMEMLRSGAFQ"
     gene            461..2722
                     /locus_tag="P5292_02545"
     CDS             461..2722
                     /locus_tag="P5292_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38034.1"
                     /translation="MSDFPTSTAAPSYFPGHKMYDMPPDKMTREQYQRMIGEQCLEYA
                     IHRFSQFRARQIQKMNSLYNRYNGVINEVEFNHINKTYNSDNLVRYKDYRLAKNKIDL
                     LVGEFLLRERMFKVFSMNPDARSELIDNFHFQIGLKNVSSEIGKLRKNGVPILDGMEP
                     LQMDDDAIFDMLSSKRKNNVIMQLLLEKGMEQEDIFVKLASNLKDIAISSECYGKVYI
                     DAAGYTRYREIDPRDRLAEETDRDPFLLRSPYLGERRLMFPHDIYANWEFTPQEKKKL
                     EEDIANFRGSSSNGTATISRYNGFQWVANQLQVETYTIEWLAVEPVIIKETPRKLGGV
                     LKSEMSFDYYQRKGKQIRNEVKNGKYKIRVYPLAYLWEASRIGRSITKNVRVVPDQSR
                     DNSNPFLANYSYKSLVFGTHDGVRISVLNALDHLSELYNLTWLSIRRELNKAKGKLLA
                     YNRAMLPAGKQIEQVIGRMVNDSIIDLDTSTDQAQFSGGAQSLANFLKEFDLGLSQNF
                     PQMLELKREIERTSEVLIGVGGPREGRTPASMTATNAVNQIQISRTSTEYLFTMHHQF
                     CKKVIKDMLQKLKITYGQHHPEEARRLLGDKMAMYLQNTKELFLDDWDAYITDGRKEM
                     EIRDMMREWFPQAVNAGELRVEDAMEAAMKESIDMAIGVLRKGRQLMEQQRQQEAQMA
                     SQEKQNITQAQLGAAAEQADKERKFKVFMEMLKALLEQGKITQQAMNDYTVMAQEMIG
                     TQPEASNQMEMIG"
     gene            2719..3096
                     /locus_tag="P5292_02550"
     CDS             2719..3096
                     /locus_tag="P5292_02550"
                     /inference="COORDINATES: protein motif:HMM:NF039553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5856 family protein"
                     /protein_id="HRS38035.1"
                     /translation="MKEYASICGFLVWMQTSIKLTHWSQKGIGSEARHEALGDLYQTL
                     DGKIDQLVESLQSYAGILELRVPEAATKAKDHAETLKFLEAIRSQLKTFKVNIAQMSE
                     IANLYEEIILAVSQTIYKIKILS"
     gene            3093..3410
                     /locus_tag="P5292_02555"
     CDS             3093..3410
                     /locus_tag="P5292_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38036.1"
                     /translation="MSKIRIPNRNMGSLMGTVKAVVIDKTAKPVDEYSWDVPPDNRPS
                     FQLNGRDFPAVQNWKVGERYKLEIEVEMTSSKAVDTKGGNTIVANFRINTIKEEEPYE
                     GQQ"
     gene            3417..4628
                     /locus_tag="P5292_02560"
     CDS             3417..4628
                     /locus_tag="P5292_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38037.1"
                     /translation="MKKLYRYYNAGPGAGESGGAAAPAAETPPAGSESASQGQQQPGG
                     NGITSFAELDLPPEATIDDIERALKSGVPAEKKEGDKNGEGEILKNPPKTEAGEEFVL
                     DIKEDLGEAGSENDSAEATWIDMSTALELEAPEEDSFDGFKSAYQKKLDAVKAEAREE
                     GKKEYTEKIAAMSPEAKALFDFLLIEGNTVKNFMQPTRQIDEYLAMDDEALIRADLKA
                     RNYDEEMINAKLDELKLDNKTKIHAYDIRKTLEVIRAKKEEQIVSNARAALQAKEQQK
                     LEKAKKELEDFQGALTKQNDLWGVPVSPKAKEVILQRFKAGQYRTRLEQDPDLAAEIA
                     LLIEFKDSLKSQLLGNAKTEGKESLLKKVHAVSLGQQSSASGKPSDDKLEGFDVWEQK
                     LAEEKASQKGE"
     gene            4693..>5199
                     /locus_tag="P5292_02565"
     CDS             4693..>5199
                     /locus_tag="P5292_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38038.1"
                     /translation="MKIQTIQGKWDDTCTQSNNLTENAIKKPYLREIMEYADGRALST
                     LIVSGAKSPWDLRTGDTDAIKTQIGKIPDGHQIGDHGMRYKVMGRIQAPAVVVAQVGS
                     TNADGTFVLTMKDQQLYPGQMVKFYRDNFYARVQGQPVPAAGGWNYVFRNTSELFDAN
                     VHLHPVGDK"
BASE COUNT         1627 a         1199 c         1281 g         1092 t
ORIGIN      
        1 ggccattgtc gttcgaatcg gatgaaagtg agcagcgtca tgactttggt atcatgatga
       61 cgcaaagccc caaaagcaaa ccgcaggcct tgtctatgct tcagacgtac gtcctggacg
      121 agattgacga atgctatttt gatattgcca ttgaagaact ttccggatat gacgagctgg
      181 tcacagactc ggactttgac ctggttgatg ccatgctgtg cgggatagcc tgtgatatcg
      241 atatgaagct cgcaaaggtt cagaagccaa ctgataagcc ggtaatggaa caacaatggg
      301 tctataacca gcagtcaggc acgtatcaat tgaaacaagt cgcagtgtat ccttccagca
      361 aggatgatga acaagacaaa gagcaacggt tcaaagaccc gttcatggaa atgcttcgat
      421 ccggggcttt tcagtaaccg ttttttttta aattagccgc atgagcgact tccctacgtc
      481 cacagctgcg ccctcatatt ttccggggca caagatgtac gacatgccac cggacaagat
      541 gacgcgggag cagtatcagc gtatgatcgg tgagcagtgc ttggaatacg ccatacatcg
      601 gttttctcag tttcgcgcac ggcaaattca gaagatgaac tcattgtaca atcgatacaa
      661 tggcgtcatc aatgaagtcg aattcaacca catcaataag acctacaatt cggacaacct
      721 ggttcgttac aaagactacc gtcttgcaaa gaataagatt gacctcttag taggagaatt
      781 tttgctgaga gaacgcatgt tcaaggtgtt ctctatgaac cctgatgcgc gaagtgaact
      841 cattgacaat ttccactttc agattggact gaaaaacgtt tcttcagaga taggaaagct
      901 gcgcaaaaac ggcgttccca tacttgacgg aatggagcct ttgcaaatgg atgatgatgc
      961 catatttgac atgctatcat ctaagcgcaa aaacaatgtg atcatgcagc tccttctcga
     1021 aaaaggaatg gagcaggagg acatctttgt caagctggca tcaaatctga aagacatagc
     1081 aatctcttcg gaatgctacg ggaaggttta cattgacgct gccggctata cacgataccg
     1141 ggaaatcgat ccgcgcgatc gcttggcaga agagacggat cgagatcctt ttctgcttcg
     1201 ttcgccttat ctgggcgagc gccggctgat gttccctcac gacatttatg ccaactggga
     1261 atttacgcca caagagaaaa aaaagctcga agaagatatt gcaaatttcc gggggtcgag
     1321 cagcaatgga accgcaacca taagtcgata caacggattt caatgggtcg ccaatcagct
     1381 ccaggttgag acatacacaa tcgaatggtt ggcagttgag cctgtgatca ttaaagagac
     1441 tccgcggaag cttggcggcg ttcttaaaag cgaaatgagc tttgactact accagcggaa
     1501 aggaaaacag atccgcaatg aagtcaagaa cggcaagtac aaaatccgtg tttatccatt
     1561 ggcttacttg tgggaagcct ctcggatagg tcgctcaatc accaaaaatg tacgagtcgt
     1621 tccggatcaa agccgcgata attcaaaccc gtttttagca aactactcat acaaatcgct
     1681 cgtatttggt actcacgacg gagtccgaat atcagtgcta aatgcgctgg accacctgtc
     1741 tgaactttac aacctcactt ggttaagtat ccgaagggaa ctcaacaaag ccaaaggaaa
     1801 gctgctggct tacaaccggg ccatgttgcc tgcgggcaaa caaattgagc aggtaattgg
     1861 tcggatggta aacgatagta ttatcgatct tgatacatca actgatcagg ctcaattcag
     1921 tggcggcgct caatcgttgg ccaattttct taaagaattc gacctgggtc tttcccaaaa
     1981 tttcccacag atgcttgaat taaagcggga aattgaacgt acaagcgaag ttttgatcgg
     2041 cgtaggcggg cccagggagg gccgtacgcc ggcaagcatg acggctacca atgcggtcaa
     2101 ccagatccaa atcagccgaa ccagtacgga atacttgttt accatgcatc accagttttg
     2161 caaaaaggtc attaaagaca tgttgcaaaa gctgaagatc acctatggcc agcatcatcc
     2221 ggaagaagcg cgtcgactgc ttggagacaa gatggctatg tatctccaaa acacaaaaga
     2281 gttgtttctt gacgactggg atgcttacat cactgatggt cgcaaggaaa tggagatccg
     2341 tgacatgatg cgcgagtggt tcccgcaggc ggtaaatgcc ggtgagctgc gtgtagaaga
     2401 tgccatggag gcagccatga aagaatcgat tgacatggcg atcggcgtat tgcgaaaagg
     2461 gcgacagctc atggagcaac aacgccagca ggaagcccaa atggcaagcc aggaaaagca
     2521 aaacattacg caagctcagc ttggcgccgc tgcagaacaa gcggataaag agcgaaaatt
     2581 caaagtgttc atggaaatgc tcaaagcgct gctcgagcaa ggcaagatta ctcagcaggc
     2641 aatgaatgac tataccgtaa tggcccaaga aatgatcgga actcagccgg aagccagtaa
     2701 ccaaatggag atgataggat gaaagaatat gcttcaatct gtggcttctt agtgtggatg
     2761 caaacgtcca tcaaactgac ccattggtct cagaaaggca ttggttcaga agcaaggcat
     2821 gaagcattgg gtgatttgta tcaaaccctc gatggaaaaa tagaccaatt ggtagagtcg
     2881 ttgcaatcct atgccgggat tcttgaattg cgcgtccccg aagccgcaac aaaagcaaaa
     2941 gaccacgctg aaacgctaaa attcttggaa gcaattcgaa gccaacttaa aactttcaaa
     3001 gtgaacattg cccaaatgtc tgaaattgca aacctgtatg aagagatcat acttgcagtc
     3061 agccaaacta tttacaaaat caaaatcttg tcatgagcaa aatacgcatt ccaaacagaa
     3121 acatgggttc cctgatggga accgtaaaag cagtagtcat tgacaagact gcaaaaccgg
     3181 ttgatgaata cagttgggat gtcccgccag acaatcgtcc ttcatttcag ctcaacggcc
     3241 gagattttcc ggcagtacag aattggaagg taggagaacg atataagctg gaaattgaag
     3301 ttgaaatgac ttcatccaaa gctgttgaca ccaaaggagg caatacaatt gttgccaact
     3361 ttcgaatcaa cacgatcaaa gaagaagagc cgtatgaagg ccaacaataa gaaaccatga
     3421 aaaaactgta tcgctactac aacgccggcc cgggagctgg cgaaagcggt ggagctgctg
     3481 cacctgcagc tgaaaccccg ccagcaggca gtgagtctgc aagtcaaggt caacaacaac
     3541 ctggaggtaa cggaatcact tcttttgcag aattggatct ccctccggaa gctaccattg
     3601 acgatatcga acgtgcgctt aaatcaggag tgccagcaga aaagaaagaa ggagataaaa
     3661 atggagaagg agaaatcctg aaaaacccgc ctaaaacaga agctggcgaa gagtttgttc
     3721 tggacatcaa agaagatttg ggtgaagcgg gttcagaaaa tgactccgca gaagccacct
     3781 ggattgacat gagcactgca ctggagcttg aagctccgga agaagatagc tttgacggct
     3841 tcaaaagcgc gtaccaaaaa aagcttgatg cagttaaagc cgaagcccgg gaagaaggga
     3901 aaaaggaata caccgaaaag attgcagcaa tgtcgccgga agccaaagct ttgtttgact
     3961 tcttgctcat cgaaggcaat accgttaaaa acttcatgca gccgactcgt caaatcgacg
     4021 agtacttagc catggatgat gaggcgctga ttcgtgcaga cctgaaagcc aggaattacg
     4081 acgaagaaat gatcaacgct aagttggacg agctgaagct tgacaacaaa accaagattc
     4141 atgcgtatga catacgcaaa acgctggaag taattcgcgc caaaaaagaa gagcagattg
     4201 taagtaatgc tcgagcagca cttcaagcca aagaacagca aaagcttgaa aaagcaaaaa
     4261 aagagctcga agatttccag ggggcactca ccaagcaaaa cgatctttgg ggagttcccg
     4321 tttcgccaaa agcaaaagaa gttatcttgc agcggttcaa ggccggccaa tatcgcacgc
     4381 gattggagca ggaccctgat ctggcagcag aaatagcctt actcattgaa ttcaaggatt
     4441 ctttaaaaag ccaactactc ggaaacgcaa aaaccgaagg aaaggaatcg ctgttgaaaa
     4501 aggttcacgc cgtgagtctc ggacagcagt cgtctgcctc cggaaagcca tccgatgaca
     4561 agttagaagg ctttgacgtg tgggaacaaa aactcgccga agaaaaagca tcccaaaaag
     4621 gagaatgagc atgaggcaac ccccctcaat tacatccttc aggtgcgcaa cactcaaaac
     4681 ctgttgtaaa ccatgaagat tcaaacgatc caagggaagt gggacgatac ctgtacccaa
     4741 tccaacaacc tgacggagaa cgcgattaaa aaaccgtatc tccgcgaaat catggagtat
     4801 gctgacggcc gcgccctcag cacgctcatc gtatccggcg caaaaagtcc ctgggacttg
     4861 cgtaccggtg acaccgacgc gatcaagacc caaatcggca agattccgga tggccaccaa
     4921 atcggcgatc acggtatgcg ctacaaagtc atgggccgta tccaggctcc ggcggtcgtc
     4981 gtagcacagg tcggcagcac caatgctgac ggaacctttg tgctgaccat gaaagaccag
     5041 cagctctatc cgggccagat ggtcaaattc taccgcgaca atttctatgc tcgtgtccag
     5101 ggtcagcctg tgccggcagc cggtggctgg aactatgtgt tccgtaacac cagcgaactg
     5161 ttcgacgcaa acgtgcacct gcacccggtg ggagacaag
//
LOCUS       DAOTON010000080         5181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13339_length_5181_cov_3.284237, whole genome shotgun sequence.
ACCESSION   DAOTON010000080 DAOTON010000000
VERSION     DAOTON010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13339_length_5181_cov_3.284237"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2782)
                     /locus_tag="P5292_02570"
     CDS             complement(<1..2782)
                     /locus_tag="P5292_02570"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS38039.1"
                     /translation="MGRKLYVLLLLLLCLCGVSYAQTGEIRGRVTEKGGAEGVPFASV
                     AAMLNGVQVQATLTDFDGNYVIKPLNPAKYDVKVTCVGYTAKETRGVLVTTDKAAFVS
                     FEMTKGVELKAVEITEYTVPLIDKGSPSTKKTVTYEEIQAAPTRDVNSLASTTAGVYQ
                     KDEGGALNIRGSRDDATTYFVDGVRVRGGTGLPQRGIEQITVTTGGLEAQYGDVTGGV
                     IAITTRGASPVYSGGLEFVTSEFLDSYGYRLATGSVSGPIYTKRDSAGKKFGQPLAGF
                     FLAAEYQYDKDPSPSSIPIYQVKDDILESAKSNPLVRDPSGFTYIHRSHYWTYDSLDK
                     VKARPNVSADAYRFNGRLDLQPAKNVTVAFGGSYERSKSRDFIDIYSLMNYDNNPQTI
                     NTNWRAWGRITQRFVPETKEGASSALKSAYYSIQADYSKNLSTSQDYRLKDDIFRYGH
                     VGKFTTYSVPFYLGEAITRPGQSDSIVFTQIANFDTLYDYQASNDNPDISAYTTQYYD
                     LTDPLGTPGYQDSYGTLLVRGALVNGDNRNPLNVYGLWATTGRLRNFYGVTDNTQFRL
                     TARGSVDIKNHNIVAGIEYEQRADRGWSVNPSNLWSLMRQLANGRFTGYDTTTAIITS
                     TTSGGSPITFIAYPDATYSPTTNADGETAPGFFENIRDRLGIADNDTVLVDALDPSQL
                     SLDMFTPDELVDFGFVNYYGYDYTGNKLTSQPAYDDFFTKKDDKNNFLREVDAFRPNY
                     VAGYLQDRFTFNDITFNVGIRVDRFDANQKVLADKYLLFRSYTAGDPQVTSRIPNAVI
                     PDNIGSDYTVYVNNVQNPTAIVGFRNEDQWYDAQGTPITDLTPLLDNSTGGSGIQPFV
                     VNYDDVQKGRIRTDVFKDYSPQVNVMPRIAFSFPISDEAILRAHYDLLTQRPQNSGLL
                     RSNPVSYLFLARG"
     gene            3127..>5181
                     /gene="smc"
                     /locus_tag="P5292_02575"
     CDS             3127..>5181
                     /gene="smc"
                     /locus_tag="P5292_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRS38040.1"
                     /translation="MRLTTLEIKGFKSFGEKVTIHFDKGVTSIVGPNGSGKSNVVDAI
                     RWVLGEQKTRMLRSEKMENIIFNGTKSRKAANVAEVSLTFENTKNILPTEYSTVTISR
                     RYYREGESEYLLNGVTCRLKDITELFLDTGIGSDSYAIIELKMVDEILNDKNNAIKNL
                     LEEAAGISKYKIRKKQTFQKLEDTEGDLNRVNDLLFEIERNMKSLESQSKKTERYYRL
                     KEQYKELSTELAVFTLSGSKQTFDELQQQEVQQVEQKVALDTQLDTSEAELQQKKLLA
                     LDREKELTESQKKLNLKVDTIRSQENDKKVSNEKLKYLQDKEQTLTQQISSDRANLQT
                     TQDQIRQSETDRLQEESLLDQLQAQLQEMKTALDVVRSRHDEMKSDIHRFTTESQSIQ
                     TAMHETEKTLAVTQARKESLQKEIERLGSDTSHKEQELGRISSDTDQSRLNKDNREQE
                     LNSLLEREVQIGADLTRYEEELKKANESLMAEGRKLDARQNEFNLTKSLVENLEGYPE
                     SLRYLRKNAPDALRAPLFSEILSCQAEFKTAIENYLDPFMNYFVIEDIEAASQAVNLL
                     AQAAKGRANFFILQNFEGSASTDSNGPQGTIPALSILDVEPRYQALCNHLLRNVYIVV
                     EDEDRTPFLRESQEDRIFISRNGKYCRTRHALSGGSVGLFDGKRIGRAKHLDTLSKEI
                     EQL"
BASE COUNT         1336 a         1382 c         1379 g         1084 t
ORIGIN      
        1 ccccacgggc caggaacaga tagctaaccg ggttggagcg cagcaatcca ctgttctggg
       61 gacgctgggt cagcagatca tagtgcgcgc ggaggatcgc ctcgtccgag atcgggaatg
      121 agaaagcgat acggggcatg acgttgacct gaggagagta gtccttgaaa acatccgtgc
      181 ggatacggcc tttttgtacg tcatcatagt tgacgacaaa cggctgaata ccgctaccgc
      241 cggtactgtt atccagcagc ggtgtaaggt ccgtgatcgg tgttccctgt gcatcgtacc
      301 attggtcttc attgcggaaa cctacaatgg cagtcgggtt ctggacattg ttcacgtaca
      361 cggtgtagtc ggaaccgatg ttatccggga taacggcatt cgggatgcgg ctggtcacct
      421 gcggatcgcc ggcagtgtaa ctgcggaaca acaggtattt gtcggccaga accttctggt
      481 tagcgtcgaa ccgatcaacg cggataccga cgttgaacgt gatgtcgttg aacgtaaagc
      541 gatcctggag ataacccgca acgtagttcg ggcggaaagc gtcgacttca cgcaggaagt
      601 tgttcttatc atccttcttc gtgaagaaat cgtcgtaggc cggctggctg gtgagtttgt
      661 tacccgtata gtcgtaaccg tagtagttga caaatccgaa atcaaccagt tcgtccgggg
      721 tgaacatatc gagtgacaat tgggacggat ccaaagcgtc gaccaggaca gtatcgttgt
      781 cggcaatgcc caggcgatcg cggatattct caaaaaatcc aggagccgtt tcaccatcag
      841 cgttcgtggt cgggctatac gtggcatccg gataagcaat gaacgtaatc ggggatccgc
      901 ctgaagtggt cgaggtaatg atcgccgtgg tggtatcgta accggtgaag cgaccattgg
      961 ccaactggcg catcagcgac cagaggttgc tcggattgac cgaccagccg cggtcagcgc
     1021 gctgttcgta ttcgataccg gcgacgatgt tatggttctt gatatcgacg gagccgcgtg
     1081 ccgtgaggcg gaactgcgtg ttatcggtca cgccgtagaa gttacggaga cgaccggtgg
     1141 tagcccaaag tccatagacg ttgagcgggt tccggttatc accattcacc agcgcgccgc
     1201 ggacgagcag cgtaccataa gaatcctgat aacccggtgt gccgagcgga tcggtcagat
     1261 cgtaatactg ggtcgtgtag gcagaaatgt ccgggttgtc gttgctcgcc tggtagtcgt
     1321 agagcgtatc gaagttggcg atctgggtaa atacaatgct atccgactgg cccgggcggg
     1381 tgatcgcttc gccgaggtag aatggaacgc tgtaagtggt gaatttacct acgtgtccat
     1441 accggaaaat gtcgtccttc agacggtaat cctggctggt cgaaaggttt ttcgaatagt
     1501 ccgcctgaat ggaatagtaa gcactcttga gtgcggacga agcaccttct ttggtctcag
     1561 gaacgaatcg ttgtgtaata cgaccccagg cgcgccaatt ggtattgatg gtctgcgggt
     1621 tgttgtcata gttcatcagc gaatagatgt cgatgaaatc acgactcttg gacctctcat
     1681 aggaaccacc gaaagcgacc gtaacgttct tggcgggttg aagatccaaa cgaccattga
     1741 agcgatacgc atccgccgaa acgttcggac gggctttcac cttatccaag ctgtcgtagg
     1801 tccagtagtg tgaacggtgg atataggtga aaccactcgg gtcacgcacc aacgggttcg
     1861 acttcgcact ttccaggata tcgtccttca cctgatagat cggaatggaa gaaggcgaag
     1921 gatccttgtc gtattgatat tcggccgcca ggaagaatcc tgccaacggt tgtccgaact
     1981 tcttgcctgc tgagtcgcgc ttggtataaa tcggtccgga taccgaaccg gtggcaagac
     2041 gatagccata cgaatcgagg aactcggaag tcacgaattc caaaccgccg ctgtaaaccg
     2101 gagaagcacc acgggtggtg atcgcgatca caccaccggt aacgtcaccg tattgggctt
     2161 ccaaaccacc ggtcgtcacc gtaatttgtt cgataccacg ctggggaaga ccggtaccgc
     2221 cacgaacgcg tacaccgtcg acaaagtacg tggtagcatc atcacgcgaa ccgcggatgt
     2281 tcagcgctcc accttcgtcc ttttgataca caccggcggt tgtagaggca agcgagttga
     2341 cgtcgcgtgt cggagccgcc tgaatttctt cataggtaac ggtcttcttg gtcgaaggcg
     2401 aacccttatc aatcagcgga acggtatatt ccgtgatctc aactgctttc aactccacac
     2461 ccttcgtcat ttcaaaagaa acgaatgcgg ccttatcggt cgtgacgaga acgcctcgcg
     2521 tttctttggc cgtgtaaccg acacaggtca ccttgacatc gtacttcgca gggttcaacg
     2581 gcttgatgac atagttaccg tcaaaatccg tcagtgtcgc ctggacctga acaccgttga
     2641 gcatggcagc cacgctggcg aacggaactc cttcggctcc gcctttttcc gtgacacgac
     2701 cacggatctc accggtctgc gcatagctga cgccgcagag gcaaaggagc aatagaagga
     2761 gtacgtatag ctttcgtccc ataggatgga tttgttagca ggtagttaat tttcgtgggc
     2821 ggtaaatgta tgaattgaat agttaaaacc aaaaacaacg catttttccg acattcgatg
     2881 acgaccttca tgctgagtgg gttagcaatt attccggcag aaaatacttc cgtgtaattg
     2941 ttaaagccag tcactttctt ccacccaaag aaaaggcgac gtttttcgaa ttctgtcaaa
     3001 cgctcgtcag cttatttacc gcacatgaat taccccgtca tcacagggtc aagagttatt
     3061 aacaaatcct tctgaagccc catcaaatgg cttatttttg ccagttcacg actcgacaca
     3121 cactttatgc gtcttacaac actagaaatc aagggattta agagtttcgg cgagaaggtg
     3181 accatccact tcgacaaggg ggtcacttcg atcgtaggtc ccaacggtag cggcaaatcc
     3241 aacgtggtgg atgccatccg ttgggtactg ggcgagcaga agacgcggat gctgcgctct
     3301 gaaaagatgg agaacatcat tttcaacggc accaagagcc ggaaagccgc gaacgtcgcc
     3361 gaagtctccc tgacatttga aaacaccaag aatatcctcc cgacggagta ttccaccgtc
     3421 acgatatccc gccgctatta ccgcgaaggc gaaagcgaat acctgctcaa cggggttacg
     3481 tgtcgtctca aggacattac cgaacttttc ctggacacgg gtatcggttc ggactcctat
     3541 gccatcatcg aattgaagat ggtcgacgag atcctcaacg ataagaataa cgcgatcaag
     3601 aatctcctcg aggaagctgc cggcatctcg aagtacaaga tccgtaagaa gcagactttc
     3661 cagaaactcg aggataccga aggcgacctc aaccgggtca acgacctgtt gttcgagatc
     3721 gagcggaaca tgaaatcgct cgaatcgcaa tcaaagaaaa ccgagcgtta ctaccggctc
     3781 aaggaacaat acaaagaact gagtaccgaa ctggcggttt tcaccttgtc cggcagcaag
     3841 cagacattcg acgaactgca gcagcaggaa gtccagcagg tggaacaaaa ggtcgcgctg
     3901 gatacccaac tggataccag tgaagccgaa ctacagcaaa agaaactgct cgcactcgat
     3961 cgcgagaagg aactgacgga gtcacaaaag aaactcaatc tgaaagtcga cacgatccgc
     4021 tctcaggaaa acgacaagaa ggtcagtaat gaaaaactga agtacctcca ggacaaggag
     4081 cagaccctga cgcagcagat ctcttccgat cgcgccaatc tgcagacgac tcaggaccag
     4141 atccgccagt cggaaaccga tcgtttgcag gaggaatccc tgcttgacca gcttcaggca
     4201 cagttacagg aaatgaaaac cgcgctcgac gtcgtgcgtt cccgccacga cgaaatgaag
     4261 agcgacatcc accgctttac caccgaatcg cagtcgatcc agacggcgat gcatgaaacg
     4321 gagaaaacgc tcgcggtcac ccaggcgcgg aaagaatccc ttcagaagga gatcgaacga
     4381 ctgggctccg acaccagcca caaagagcag gaactcggac gcatcagctc cgataccgac
     4441 cagagccgcc tcaacaaaga caaccgggaa caggagttga attcgctgct tgaacgcgaa
     4501 gtacagatcg gcgccgacct gacgcgttac gaagaagaat tgaaaaaggc gaatgagtcg
     4561 ctcatggccg agggtcgtaa gctggacgcc cgccagaatg aattcaacct caccaagagc
     4621 ctcgtcgaga acctcgaggg ttatccggaa tcgctgcgtt acctgcgcaa gaacgcgccg
     4681 gacgcacttc gcgctcccct tttctcggaa atcctgagct gccaggccga gttcaagacg
     4741 gcaattgaaa attacctcga tccgttcatg aactatttcg tgatcgagga tattgaagcc
     4801 gccagccagg cggttaacct gctggcacaa gccgcgaaag gccgcgcgaa tttcttcatc
     4861 ctccaaaatt tcgaaggaag cgcctctacc gattccaacg gcccgcaagg caccatccct
     4921 gccctctcca tcctcgacgt agagccacgc taccaggccc tttgcaatca cctcctgcga
     4981 aacgtataca tcgtcgtgga agacgaggac cgcacaccct tcctgcgtga aagccaggag
     5041 gaccgcatct tcatttcccg caacggcaaa tactgccgta cccgccacgc cctttccggc
     5101 ggctcggtcg gcctcttcga cggaaagcgc atcggacgcg ccaagcatct ggatacgctt
     5161 tccaaagaga tcgaacaatt g
//
LOCUS       DAOTON010000081         5127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13503_length_5127_cov_3.422713, whole genome shotgun sequence.
ACCESSION   DAOTON010000081 DAOTON010000000
VERSION     DAOTON010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13503_length_5127_cov_3.422713"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..801
                     /locus_tag="P5292_02580"
     CDS             43..801
                     /locus_tag="P5292_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HRS38041.1"
                     /translation="MNIPVRISLVSYLNSRPFLYGLRHSTLARSIELQLDNPAVCADK
                     LTQGSVDIGLVPAAILPKLPDYQIVSEYCIGAFGPVESVLLVSDVPLESISSVLLDYQ
                     SRTSVSLVRILAEQLWHITPEWHPATPGFEEKIAGSQAAVIIGDRAFKAARSHPYVYD
                     LAEAWTELTGKPFVFAVWASRTRLDAEFISAFNAALSFGLSHLDQVVSAETETALSPE
                     ALRKYLTHSIRFNFDSAAQEGLAEFLHRQSVSLV"
     gene            complement(864..2153)
                     /locus_tag="P5292_02585"
     CDS             complement(864..2153)
                     /locus_tag="P5292_02585"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRS38042.1"
                     /translation="MKNLVLAGLLLAGTLYVGSSNGQTGNAHPIRCNTTDYMNAKMAA
                     DPAYARQFEMDEQTLQRAIANLANNRSSSSNSIVTIPCVVHVVYRTVGQNISDAQVES
                     QIDVLNEDFARLNADTNVTPAPFRAAASGSQFRFCLAQTDPNGNPTTGIERRQTTVNT
                     FSTNDLVKAYSTGGMDAWDTDVYFNIWVCSLGGGILGYAEFPAASHTDTYGVVITYSA
                     FGRVGLVAAPYDQGRTCTHEVGHAFRLNHIWGDDGGSCGGTDYVADTPNQAGETYGCR
                     TFPHTDACTPSGNGVMFMNYMDYSDDDCLNMFTTGQVTRMTTAMNTFYSSLLVSTRCE
                     SANSIQDGPENFSFSVYPNPTNGVINLDMFLTRPIGDQAVVRVVDALGKIVYTQVLNQ
                     PSGQVHSLDLSSLPNGIYLMNVGNAEFSKTVRVSVSR"
     gene            complement(2238..2843)
                     /locus_tag="P5292_02590"
     CDS             complement(2238..2843)
                     /locus_tag="P5292_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRS38043.1"
                     /translation="MTQRWRYFSIYKPYGMLSQFTDEGGNPGLSRLEFRFPSDCYPLG
                     RLDRDSEGLLLLTNDAAVNQSLLDPRNAHPRTYWVQVEGSMGVSDCEKLSNGVIISID
                     GKEFRTRPALVELIDTPANLPDRTPPVRFRKSIPTSWISLTLTEGKNRQVRKMTAAVG
                     YPTLRLIRVAVGKLELPEWKPGSVTEWSSSDFFEKTGIKKA"
     gene            2994..3410
                     /locus_tag="P5292_02595"
     CDS             2994..3410
                     /locus_tag="P5292_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38044.1"
                     /translation="MRSISPFVASVIAVIFVLSSSSCKKEDKCIAGTGGNLTIVAKLR
                     HHGTLIPNDSLKPDTVWIKFNAKDWGGAPSGYDMRVIGEFPEDHVHLSGLKCGDYYFY
                     ASGWDTSIVQLVKGGIPFSTDQESGEIVLDIPVTED"
     gene            3420..3818
                     /locus_tag="P5292_02600"
     CDS             3420..3818
                     /locus_tag="P5292_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38045.1"
                     /translation="MRSTLAIAILFLLLFQTGCRKEAVNPDGKVIVEGTIRLQVSAVH
                     HSWGVPGIRIYLKKGATEFPGYDPALYEYAIQADNEGLGAFEKLYPGKYFVYAKGWDY
                     YWGDTVIGYAPVVLTDPTRLDNAMTLYVSE"
     gene            3901..4380
                     /locus_tag="P5292_02605"
     CDS             3901..4380
                     /locus_tag="P5292_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38046.1"
                     /translation="MAETGFPESVDKIISGKCATAGCHNDISYQNAGGLDFSTWDVTF
                     RGGRNGSSIIPYSTLYSYCLYFVNTDSTRGPVLEPTMPYQAAPLSTAEYQTLYDWIAN
                     GAPNKDGFVKYSDDPDREKVYICMQGCDQVAVFDAASQNIMRYIPVGNDPGQIEAPH"
     gene            4381..>5127
                     /locus_tag="P5292_02610"
     CDS             4381..>5127
                     /locus_tag="P5292_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38047.1"
                     /translation="MRVSPDGVYWYVVFYAGSVLQKFRTSDDSLVATFNIGFGDWNTI
                     IFTPDGSRGFVNGTNINTTAVVDLVNNTVITQISPELPHGGFVTPDGHWLYLTSQVSN
                     FINKVDITDPGFSFDPVILQPGEPKLTTSRYDPHEAVLSPDGTKYFVSCQTSDEIRVF
                     RLSDDSLLKIIPVGTKPQEFAESTNKPYLYITCTEEVVDVNRKGLVYVINYNTLELVD
                     SIYTGYQPHGIDFDPVRKLVYVANLNVDNTG"
BASE COUNT         1227 a         1325 c         1323 g         1252 t
ORIGIN      
        1 taccctgcat gacggagaga tcgtaccttc gccggtaacc gcatgaacat accggtccgc
       61 atatcgctgg tatcctacct gaacagtcgc ccgttcctct atggtttgcg ccattccacc
      121 cttgcgcgct ccatcgaact ccaactggac aatccggccg tctgcgccga caaacttact
      181 cagggaagcg tggacatcgg attggttccg gcagccattt tgccaaagct accggattat
      241 cagatcgtca gtgaatattg cattggagct tttgggccgg ttgaatcggt actcctggtt
      301 tccgacgttc cgctggaatc catttcctcc gtcttgctgg attaccaatc gcgcacctcc
      361 gtatcgctcg tgaggatcct tgcggaacag ttgtggcaca tcacccctga atggcatcct
      421 gccactcccg gcttcgaaga aaaaattgcc ggcagtcagg ccgccgtcat cattggagac
      481 cgcgctttta aagctgcaag atcacatcca tacgtttacg atctggctga agcatggact
      541 gaattaaccg ggaagccctt tgtctttgcc gtatgggcca gtcgcacccg gctggatgcg
      601 gagttcattt ccgcattcaa cgcggcatta tccttcggct tgtcccatct ggatcaggta
      661 gtaagtgccg aaaccgaaac ggcactatct cctgaggcgc tacggaagta tctgactcat
      721 tccatccggt tcaatttcga ttcagccgcc caggagggcc tggctgaatt tctacatcga
      781 caatcggttt cgctcgtctg atttcttaaa taagaaaggc cgctcagagg agcggccttt
      841 cttatttacg gtaattcaga aaatcaacgg gagacgctaa cgcgtaccgt ttttgaaaac
      901 tcggcattcc cgacattcat caggtaaatt ccgtttggaa ggctgctaag gtcaagcgaa
      961 tgcacctggc cggaaggttg gttgagcact tgcgtataga cgatctttcc cagcgcatcc
     1021 acgacccgta caacagcctg atctccgatc ggacgcgtaa ggaacatgtc gaggttgatc
     1081 acaccattcg taggattggg gtaaacgctg aacgagaagt tctccggacc gtcctgtatg
     1141 gaattagcgg attcacaacg cgtagaaacc agcaaggagc tgtagaacgt gttcatggcg
     1201 gtcgtcatgc gggttacctg acctgtggtg aacatattga gacaatcgtc atcggaataa
     1261 tccatatagt tcatgaacat aaccccgttt ccgctcgggg tgcaggcgtc cgtgtgcggg
     1321 aaggtgcggc aaccataggt ttccccagcc tggttgggag tatcggcaac gtaatcggta
     1381 ccgccgcagc taccaccgtc gtcgccccag atgtggttca gacgaaaagc gtgaccaact
     1441 tcatgggtac aggtacgtcc ctgatcgtac ggagcggcaa ccaagccaac tcgtccgaaa
     1501 gcagaatatg taatcacgac gccataggta tcggtatgtg aagcagccgg aaattccgca
     1561 taaccgagga taccaccacc gaggctacaa acccagatgt tgaaatacac atccgtgtcc
     1621 caagcatcca ttccgccggt cgagtatgcc tttaccaggt cattcgtgga gaaggtattg
     1681 accgttgtct gacggcgctc gatgccggta gttggattac cgttaggatc ggtttgagcg
     1741 agacaaaagc gaaactggga acccgatgca gccgcccgga aaggagccgg agtgacattc
     1801 gtatcggcgt tgagacgggc gaagtcttcg ttcaacacat caatctggga ttcaacctgc
     1861 gcatcggaaa tattctgtcc gacggtgcga taaacaacgt gcacgacgca aggaatcgtc
     1921 acaatcgagt tggaagaaga cgatcgattg ttagccaggt tggcaatcgc gcgttgcaat
     1981 gtctgctcgt ccatttcgaa ttggcgcgca taagccggat cagcggccat cttggcattc
     2041 atatagtccg ttgtgttgca gcggatgggg tgcgcatttc cggtttgtcc gtttgacgaa
     2101 ccgacataaa gagtgcctgc cagcagcagg cctgcgagta caagattttt catgtaatag
     2161 ttgatggttg gcaagtattt aacaaatata gggaatcggc ttgtaaggtg ggtgaagcta
     2221 gccgtaagct ctaagaatca ggccttcttt atacccgttt tttcaaagaa atcagaagat
     2281 gaccattcgg tgaccgaacc gggtttccat tccggcaatt ccaacttacc cacggctacc
     2341 cgaatcaggc gaagggtcgg gtagccgact gctgccgtca tctttcgaac ctgtctgttt
     2401 tttccctccg ttaacgtcag cgagatccag gatgtcggaa tggatttacg aaaacgcacc
     2461 ggcggtgtcc gatcaggaag attggctggc gtgtcgatca actcgaccag tgccgggcgt
     2521 gttcgaaatt ccttcccatc aatactgatg ataacgccat tggagagctt ttcgcagtct
     2581 gacacaccca tgctgccttc cacttgcacc caataggtcc gggggtgtgc gttccgtggg
     2641 tcgagcaggc tttgattcac agcggcatcg ttggtcagca gtaacaagcc ttcgctgtcg
     2701 cgatccagcc tacccagggg gtagcaatcc gacggaaacc tgaactccag gcgggacaaa
     2761 cccgggttgc ctccctcatc ggtgaactgc gacaacatcc cgtaaggctt gtaaatgctg
     2821 aaataccgcc agcgttgtgt catatgtttg aaggaacgtc aggattccgc cagggttgca
     2881 gtgtgggttg aagaaattgt ggttaacttt agggtgcgaa tcattgttaa gcagatctgc
     2941 gtatcaactg ttggagcatt tctttgcttc agtttaaatc tacttaagtt caaatgcgat
     3001 caatctcccc cttcgttgcg agtgtaattg cagtaatttt tgtgctttct tcctcttcat
     3061 gtaaaaagga agacaagtgc attgcgggta ccggtggcaa cctgaccatt gtcgccaagc
     3121 ttcgccacca tgggacgctg atcccgaatg attcattgaa accggatacc gtctggataa
     3181 aattcaacgc aaaggattgg ggcggtgcgc caagcggata tgacatgcgg gtcatcggtg
     3241 aatttcccga agaccatgtc catctatccg gtttgaagtg tggagactac tatttttacg
     3301 cctccggttg ggacacctcg atcgttcagt tggtgaaggg cggcatcccc ttctccaccg
     3361 atcaggaaag cggcgaaatt gttttggata ttcctgtaac ggaagattaa accgaggttg
     3421 tgcggtccac tctcgctatc gctatacttt tcctgctcct gtttcaaacc ggttgcagaa
     3481 aggaagcggt caaccccgac ggaaaagtga ttgtcgaggg aacgatccgg cttcaggttt
     3541 cagcagtgca ccatagttgg ggcgttcccg gtattcgcat ctatctcaag aaaggcgcga
     3601 cggaatttcc gggttatgat cccgcgctgt acgagtatgc aatccaggcc gacaatgaag
     3661 gcttgggagc attcgaaaag ttatatcctg gcaagtactt cgtatatgcc aaaggatggg
     3721 attattactg gggcgatacg gtcatcggat atgccccggt cgtgctcacg gaccctactc
     3781 gactcgataa tgccatgacc ctctatgtca gtgaataatt cgcatcgctt cctaatctta
     3841 ttgggtttag taacggtgtt cagcacgcat gcctgccggt acgatgaact tccctcacca
     3901 atggcggaaa ccggcttccc ggagtcggta gacaaaatca tttcaggaaa atgcgcaaca
     3961 gccggttgcc acaatgatat cagttatcag aatgccggag gtctggattt ttcaacctgg
     4021 gatgtcacct tccgtggcgg ccgcaatggc tccagtatca ttccttacag caccttatac
     4081 agctattgtc tttatttcgt caataccgat agtacgcgtg ggcctgtcct ggagccgacc
     4141 atgccctatc aagctgcacc gctgagtacc gccgaatacc agaccttata cgattggatc
     4201 gcgaacggtg ctccgaacaa ggatggcttc gtgaaatatt cggatgatcc ggatcgggaa
     4261 aaagtctaca tctgcatgca gggttgcgac caggtagcgg tcttcgatgc agcatcgcag
     4321 aatatcatgc gttacatacc ggtcggtaat gatcccggtc agatcgaagc gccccattaa
     4381 gtgcgtgttt ctccagacgg agtgtactgg tatgtggtgt tctacgcggg aagtgtcttg
     4441 cagaaattcc gaacaagtga cgactccctc gttgcgacct ttaacatcgg gtttggcgac
     4501 tggaatacga tcatttttac acccgacggc tcaaggggtt tcgtgaatgg aacgaacatc
     4561 aacacgacag ccgtagtcga tctcgtcaac aatacggtga tcactcaaat ttcaccggaa
     4621 ctgccccatg gcggattcgt cacacccgac gggcattggc tttacctgac atcgcaggta
     4681 tcaaacttca tcaacaaagt tgacatcacg gatccgggct tctccttcga cccggtcatc
     4741 ctgcaacccg gcgagcccaa actgaccact tcacgatacg atccacacga agccgtgctt
     4801 tcgccggatg gaaccaagta tttcgtcagt tgccagacca gtgatgagat acgcgtcttt
     4861 cgtctttcgg atgacagttt gttgaagatc atcccggtcg gcactaagcc tcaggaattc
     4921 gccgaatcca ccaacaagcc gtatctgtat atcacctgta cagaggaagt cgtggatgtc
     4981 aaccgaaagg ggttggtgta tgtgatcaat tataacaccc tcgaactggt cgattccatc
     5041 tataccggtt atcaaccaca tggaatagat ttcgatccgg ttcgcaagct ggtgtatgtc
     5101 gccaacctga atgtggacaa tacgggt
//
LOCUS       DAOTON010000082         5124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13519_length_5124_cov_2.820477, whole genome shotgun sequence.
ACCESSION   DAOTON010000082 DAOTON010000000
VERSION     DAOTON010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13519_length_5124_cov_2.820477"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1594
                     /locus_tag="P5292_02615"
     CDS             194..1594
                     /locus_tag="P5292_02615"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRS38048.1"
                     /translation="MLFNSLAFSLFFPLVTFGYFLLRPGQRWWWLLLASCVFYMYFRP
                     VYILVLALTIGIDYAAGLLMERSSDFRVRRRWLGASLIANIGILAFFKYWGFLDENLA
                     ALTGWTGSPLRLPVLDVVLPVGLSFHTFQAMSYTFDVYYGRQKAERHLGIYALYVMFY
                     PQLVAGPIERPGQLLPQFREHHTIDYGRISKGLVRMLWGLFKKIVIADRLSLFVDPIF
                     REPEGYGGITMVWAVLFFSIQIYADFSGYCDIALGAAQVMGFRLMENFRHPYFSASFR
                     EFWTRWHISLSQWFRDFVYIPLGGNRVPIVKEIRNLLIVFALSGLWHGASWTFVVWGL
                     IHGFFLITERLLFPNGRSSTILNRLLTFTGVCLAWVFFRADSFDDAMQVFAGLFRPSA
                     GWIGLPLITTQSFITDLLLLLLLFLAEWRQWHQPGTASAAPSRRWAAYGWHAALAVLI
                     LLYGVFEAQSFIYFQF"
     gene            1596..2558
                     /locus_tag="P5292_02620"
     CDS             1596..2558
                     /locus_tag="P5292_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38049.1"
                     /translation="MRRLILELFLLLAFMGVVYGLLEWRYRDYSIGLDGQYAQFRMEQ
                     TQAEVLFLGNSHIIPLKAASDSMRKVRSASLAFVGMDLFWTKVLLNRQQDALPRLKEV
                     ILYADPEILGYNQTLHGQAWINRALYRYGDTLYNDRLSDRWMARSNFFRSNRDLSYLF
                     RERNGSAELPIVRDRLSLPLDDTIACKVRAIENSRSRFDERLFPENLRYLNTILDCCK
                     QHGWQVTLVRTPKRSAFLRYFEHAGIERARLKLDSLAKARGLFLFEPGWSAEDDRLIA
                     DPDHLLLDGAKRFLEELDSAKEHHARESGMGNPDSISFSMTTGK"
     gene            complement(2492..3085)
                     /gene="purN"
                     /locus_tag="P5292_02625"
     CDS             complement(2492..3085)
                     /gene="purN"
                     /locus_tag="P5292_02625"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009642258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRS38050.1"
                     /translation="MTRIAVFASGSGSNAEKIIRYFQQHPAIRVCWVLTNNPNAGVIG
                     RAKALDVPCHLFDRRQFRETTAVVEFLKSEKIDLVVLAGFLWLVPANLINAFHGRIIN
                     IHPALLPQFGGKGFYGEHVHRAVIESGQPISGITVHAVNEQFDSGDILFQAACHVDRS
                     DNADQLAHKIHALEHRYFPVVIEKLIESGLPIPDSRA"
     gene            3174..3821
                     /locus_tag="P5292_02630"
     CDS             3174..3821
                     /locus_tag="P5292_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRS38051.1"
                     /translation="MSANSFIQLFVPKDRKFYPMLEQASANLVAVSKVLYELMNTSSP
                     EKRIPLFRQIEKLEHTGDEITHQIFNETSKTFITPFDREDLQRLASGLDDVLDYIHGS
                     AKRIELYKVETMHPSMVKLAELIMSCSNELYNAISGLRSMKNQMKVREALVRVNSIEN
                     HADDIFDNAVARLFEDEKDAIQIIKIKEVLSALETATDKCEDVANVIETIIVKQA"
     gene            3842..>5124
                     /locus_tag="P5292_02635"
     CDS             3842..>5124
                     /locus_tag="P5292_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PiT family inorganic phosphate transporter"
                     /protein_id="HRS38052.1"
                     /translation="MTLLITVIVLALLFDFINGFHDAANSIATVVSTRVLSPVSAVIW
                     AALFNFVAFFIFKDHSVANTIAKTVQDRFTSPEMSPLPMILAGLLAAISWNLITWWYG
                     IPSSSSHTLIGGFAGAFIAAYGFEAVRSGVVWATAIFIVVAPVAGMLMAYLISLWFLH
                     SFRKSLIAKFVSITIMVGVAWAVWNTLEFKKDFNGGASYTYMFDRKVSKDELKKALTF
                     KDDHYKEYAPVIAAGKDSLGQPDASGTVYTIRTDYLKKPGQTTELSELTVAIEQGIGK
                     TKAGYRLLETHRISEQADPKERFDTSNLKNGFETYWLKVIFHGANFKWILLGFIVMVI
                     GGTTLVLSSINMSQTNTLFKRLQLFSSAAFSIGHGGNDAQKVMGIISVALIANGNIAS
                     MKEMPTWVPLACYTAIAAGTLSGGWKIVKTMGGKIT"
BASE COUNT         1148 a         1419 c         1326 g         1231 t
ORIGIN      
        1 aggcgcgctg ccgaaccgca tggcaagcaa tgccacgatc gcgaagatca atgcgaacgc
       61 aagcgatgaa tttgccggat ttctgaacag atgacgcacg ccggtgaatg gaaacaaatg
      121 tctgttaacg ataaaacgac acagaagttc atcttctcaa aactattctt ttttttacac
      181 gccctgccga aagatgctgt tcaactccct cgccttctcg ctgttttttc ccctggtgac
      241 gttcgggtac ttcctgctgc ggccagggca acgttggtgg tggttattac tggccagttg
      301 cgtgttttac atgtacttcc ggccggttta cattcttgtt ctggcactga cgatcggtat
      361 cgactacgcc gccggtctgc tgatggaacg cagcagcgat ttccgggttc gccggaggtg
      421 gctgggcgcc agcctgatcg ccaacatcgg cattctggcg ttcttcaagt attgggggtt
      481 tctggacgaa aacctggcgg cgctgaccgg ctggaccgga tcccccttgc gattgcccgt
      541 gttggatgtg gtgctgccgg tcgggttgag ttttcatacg tttcaggcga tgagctacac
      601 cttcgacgtg tactatggcc gacaaaaagc cgaacgccat ctcggcattt acgcgttgta
      661 cgtcatgttt tatccgcaat tggtggcggg tcccatcgaa cgaccgggac aattgttgcc
      721 gcaattccgg gaacatcata cgatcgatta cggacgtatc agcaaagggc tggtgcgtat
      781 gctctggggg cttttcaaaa agatcgtcat cgccgaccgt ttgtctttgt ttgtggatcc
      841 catctttcgg gaacccgagg gttatggcgg catcacgatg gtctgggcgg ttctcttttt
      901 ctccattcag atctacgcgg atttttccgg gtactgtgac atcgcattgg gcgccgcgca
      961 agtaatgggt ttccggttga tggagaattt ccgtcatccg tatttcagcg ccagcttccg
     1021 tgaattctgg acccgttggc acatctcgct ttcgcaatgg ttccgcgact tcgtttacat
     1081 tcccctgggc gggaatcggg ttccgatcgt caaagaaatc cgtaacctgc tgatcgtttt
     1141 cgcgctaagc ggtctctggc atggcgcgag ctggacgttc gtagtctggg gactgatcca
     1201 cggtttcttt ctgatcaccg agcgactgtt attccccaac ggtcgaagtt caacgatcct
     1261 gaatcgcctg ctcaccttca cgggcgtttg cctggcatgg gtcttctttc gcgccgacag
     1321 cttcgacgac gctatgcaag tctttgccgg attgtttcga ccatcggcag gatggatcgg
     1381 tctgccgctc atcactacac agtcgttcat tacagatctg ctcctgctgc tcctgctctt
     1441 tcttgccgaa tggcgtcaat ggcatcaacc cggaacagcc tccgcagctc cttcccggcg
     1501 atgggcagct tacggctggc atgcagccct ggccgtgctg atcctgttgt acggcgtctt
     1561 cgaggcccaa tcgttcatct attttcaatt ctgacatgcg caggctgatt ctggaactct
     1621 ttcttctatt agccttcatg ggtgtggtgt atggcttgct tgaatggcgc taccgcgact
     1681 attccattgg cctcgacgga cagtacgcgc aattccggat ggaacaaaca caggctgaag
     1741 tgcttttcct cggcaattcg catatcatac ccttaaaagc cgcctcggat tcaatgcgca
     1801 aggtgcgctc cgcctcgctg gcatttgtgg ggatggactt gttctggaca aaagtgctgc
     1861 tgaaccgcca gcaggatgct cttccccgtc tgaaagaagt gatcttgtat gccgatccgg
     1921 aaatactggg ttacaatcag accttacacg gccaggcctg gatcaaccgg gccttatatc
     1981 gttacggtga caccctgtac aatgatcgcc tttccgatcg ctggatggcg cgcagtaatt
     2041 tcttccggtc aaatcgggac ctttcgtacc tgttcagaga gcggaacgga agtgccgaac
     2101 ttccgatcgt tcgcgaccgg ctctccctgc cactggacga tacgatcgct tgcaaagtca
     2161 gggccatcga aaacagcaga tcaagatttg acgaacgcct gtttccggaa aatctacgct
     2221 acctgaacac gatccttgat tgctgtaaac aacacgggtg gcaggtaacg cttgtgcgca
     2281 caccgaaacg atcggctttc ctgcgttact tcgaacatgc cgggatcgaa cgtgcgcgtt
     2341 taaaattgga ttccctggca aaagccagag gcttgtttct gtttgaaccc ggctggtcgg
     2401 ctgaagacga tcggttgatc gcggatccgg atcacctcct cctggatggc gcgaaacgat
     2461 tcttagagga acttgactcg gcaaaggagc atcacgcgcg cgaatccggg atgggcaatc
     2521 cggattcgat cagcttttcg atgactaccg gaaaataacg gtgttctagg gcgtggatct
     2581 tgtgtgcgag ctgatcggcg ttgtccgacc gatcaacatg acaggcggct tgaaaaagta
     2641 tatcgcccga atcgaactgc tcattcaccg cgtgtaccgt gatgccggaa atcggctggc
     2701 cggactcgat gacagcacgg tgaacgtgtt cgccgtaaaa acccttgccg ccgaactgtg
     2761 gtagtaaagc gggatggatg ttgatgatcc tcccgtgaaa agcgttgatc aggttggccg
     2821 gcaccagcca gagaaaaccg gcaaggacga ccaaatcgat cttctccgat tttaggaatt
     2881 cgactacagc ggttgtttcg cggaattgcc ggcgatcgaa caggtgacaa gggacatcca
     2941 gcgcttttgc acgaccgatc acaccggcat tgggattgtt ggtcaacacc caacagaccc
     3001 gtatcgccgg atgttgttga aaataacgga tgattttttc ggcattactt cctgaaccgg
     3061 aagcgaatac agcgatgcgc gtcatgcccg ccaaagatag caaatgaggg tacttgggaa
     3121 tcccgtgttc cttgcttttt cgttactttg cccccgttca acttccacac ctcatgtcgg
     3181 ccaacagttt cattcaactc ttcgttccca aagaccggaa attctatccc atgctcgaac
     3241 aggcttccgc caacctggtg gcggtttcca aagtcctgta cgaactgatg aatacctcca
     3301 gtccggaaaa acgcataccg cttttccgcc agattgaaaa actggaacat accggcgacg
     3361 agatcaccca ccagatcttc aacgaaacca gtaagacctt cattactccc ttcgaccggg
     3421 aagatttgca gcgcctcgcc agcggactcg acgacgtact cgactacatc cacggctccg
     3481 ccaaacgcat cgaactgtac aaggtcgaaa cgatgcaccc cagcatggtc aaactcgccg
     3541 agttgatcat gagttgttcc aatgaactgt acaacgcgat cagcggattg cgcagcatga
     3601 agaaccagat gaaggtccgc gaagccctgg tgcgtgtcaa cagcatcgaa aaccacgcgg
     3661 acgacatctt cgataacgcg gttgcacgcc tgttcgaaga cgaaaaagac gccattcaga
     3721 tcatcaagat aaaggaagtg ctcagcgcgc tggaaacggc caccgataag tgcgaagacg
     3781 tcgccaacgt catcgaaacc atcatcgtga aacaggccta aacggaaccg ccgaaccatc
     3841 catgacactg ctgatcaccg tcatcgtcct cgccctcctg ttcgatttca tcaacgggtt
     3901 ccacgatgcc gccaactcca tcgctacggt tgtatcgacc cgcgtcttat cgccggtcag
     3961 cgccgtgatc tgggcggctt tgttcaactt cgtcgccttt ttcatcttca aggaccacag
     4021 cgtcgccaac accatcgcca agacggttca ggaccgtttc acctctcctg aaatgagccc
     4081 tttgccgatg attctggccg gattgctcgc agccatatcg tggaatctca tcacctggtg
     4141 gtacgggatt ccatcttctt catcccatac acttattggc ggattcgccg gcgctttcat
     4201 cgctgcttac ggttttgaag ccgttcgatc cggtgttgtc tgggcaacgg ccatcttcat
     4261 cgtcgtcgcc ccggtcgcag gtatgttgat ggcctacctc atctcgcttt ggttcctcca
     4321 cagtttccga aaatccctga ttgcgaaatt cgtatccatc acgatcatgg tcggcgtggc
     4381 atgggctgta tggaatacat tggaattcaa gaaggatttc aatggcggcg cctcctatac
     4441 ctacatgttc gatcgcaagg tgagcaagga tgagttgaag aaggccctta cgttcaagga
     4501 tgatcactac aaagaatatg ctccggtcat tgccgccggt aaggacagtc tcggtcagcc
     4561 ggatgccagt ggaaccgtgt atacgataag aacggactac ctgaaaaagc ccggacaaac
     4621 aaccgaactc tccgaactga ccgtggcgat cgaacaagga atcggaaaga ccaaagcggg
     4681 ttatcgcttg ctggaaacac accgaatcag cgaacaggct gatccgaaag aacggttcga
     4741 tacttccaat ttgaagaacg gcttcgaaac ctattggctg aaggtcatct tccatggcgc
     4801 gaactttaaa tggatcctgc tcggcttcat cgtgatggta atcggcggca cgacgctggt
     4861 cctcagttcc atcaacatgt cgcaaaccaa taccctgttc aagcgcctgc aactgttctc
     4921 ttccgccgca ttctcgatcg gtcacggtgg taacgacgcg caaaaggtca tgggtatcat
     4981 ctccgtcgcg ctcatcgcga atggcaacat cgcgagcatg aaagagatgc cgacctgggt
     5041 gccgctcgca tgctataccg ccattgcagc cggcacactc agcggcggct ggaaaatcgt
     5101 taagacgatg ggcggaaaga tcac
//
LOCUS       DAOTON010000083         5049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13755_length_5049_cov_3.366840, whole genome shotgun sequence.
ACCESSION   DAOTON010000083 DAOTON010000000
VERSION     DAOTON010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13755_length_5049_cov_3.366840"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /locus_tag="P5292_02640"
     CDS             complement(<1..1540)
                     /locus_tag="P5292_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRS38053.1"
                     /translation="MGFSWQPPSSSLPEFDTQTLVSLRNSDVMALEKSLAELRKTALR
                     SDDPIRDSLLRERWIDVRVHFRLLEEWLLLTDRKLFKRWNGPPLERPEEEEAFAPILP
                     PAGLQALEPALFDSAGPQRRKELQAQFESIRILLQRLKLHATNEYSSERSYLRLCHRQ
                     LLRLFLTGLTQMETPVSRIAFEEAARTLNEQARRLSLLTQPEFESSRLALKNFVDQLA
                     TAARTLRSSGKDSEPDYYTLYTQCFLPMSDAFSRYLITRFPDWDSTDPVNPAVRSVFD
                     RNAFLLDKFMPAKHEGDHELLTAIGRMLFFDPALSSNNERSCASCHQPDKAFTDGQLT
                     SHGVQHGSVLRRNAPTLINAMLQQSLFFDLRVVNPENQAGEVLNNSEEMHGDASVIAE
                     KLQGSPGYRELFRKAFAGSEDTLITPASIVLAIAEYERRLIALNSEFDLAMRGESQLS
                     SEAISGFNLFVGKGRCASCHFVPLFNGTVPPDYWESEAEVLNVPTTSDPAHARPDPDP
                     GRAGF"
     gene            1795..>5049
                     /locus_tag="P5292_02645"
     CDS             1795..>5049
                     /locus_tag="P5292_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRS38054.1"
                     /translation="MKKPTVYVPQNKIRIVTAASLFDGHDAAINIMRRIIQSSGAEVI
                     HLGHDRSVQEVVDCAIQEDVQAIAMTSYQGGHIEYFKYMYDLLKEKGCGHIKIFGGGG
                     GVILPEEISELHAYGITRIYSPDDGRSMGLQGMINDLLKQCDFPTGINLNGEVKHLAD
                     REPRVVAKLISCAENNPDEFSKVHRDLDKLVGSKKVPVLGITGTGGAGKSSLVDELVR
                     RFLIDFSDKTIAIVSVDPSKRKTGGALLGDRIRMNSIHHPRVYMRSLATRQSNLALSK
                     YVKDAVDILKAAQYDLIILETSGIGQSDTEILDHSDVSLYVMTPEYGAASQLEKIDML
                     DFADVIALNKFDKRGALDALRDVKKQYQRNHQLWDQDPEAMPVFGTIASQFNDPGMNR
                     LYRKLIDKLNEKLEAGFESKFEITGEMSEKIYIIPPARTRYLSEISESIRNYNQWVKD
                     QAEIAQRLFAIRKSLETLDASDLAEKAPVKLALDKLYEQTELKLDGQNKKALQEWPRK
                     VQQFRDEVYTYKVRDKEVKVKTFTESLSRTHIPKIATPKFEAWGEVLRWSLQENFPGE
                     FPFTAGVYPFKRTEEDPTRMFAGEGGPERTNRRFHYVSKGMPAKRLSTAFDSVTLYGH
                     DPDYRPDIYGKIGNSGVSICCLDDAKKLYSGFNLADPKTSVSMTINGPAAILTGFFMN
                     AAIDQQCELYIRQHGLEQEVEAKIQAIYAATGTARPAYQGPLPEGNDGLGLMLLGVTG
                     DQVLPKEVYDKIKAHTVQVVRGTVQADILKEDQAQNTCIFSTEFSLRLMGDVQQYFIE
                     KKVRNFYSVSISGYHIAEAGANPITQLAFTLANGFTYVEYYRSRGMDINAFAPNLSFF
                     FSNGIDPEYAVIGRVARRIWAKAMKQKYASDERSQLLKYHIQTSGRSLHAQEIDFNDI
                     RTTLQALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINKELGLAKNENPMQ
                     GSFIIEELTDLVEEAVMAEFDRITERGGVLGAMETMYQRGKIQEESLYYETLKHTGEF
                     PIIGVNTFLSSKGSPTVIPAEVIRATTEEKEYQISMLKDLHARNEKKAVDQLKQL"
BASE COUNT         1210 a         1279 c         1488 g         1072 t
ORIGIN      
        1 gaaagccggc acgcccggga tcgggatcgg ggcgggcgtg cgcagggtcg gaagtggtcg
       61 gaacattgag cacttcggct tccgattccc agtagtcggg tggaacagtt ccgttgaaaa
      121 gcggaacgaa gtgacaactg gcgcaacggc ctttgccaac gaagaggttg aatccggaaa
      181 tcgcttcgga tgatagctgg ctttctccgc gcatggcaag atcgaattcc gagttcaagg
      241 caatcaaccg gcgttcgtat tccgcgatgg ccagcacgat ggatgccggt gtgatcaggg
      301 tgtcttcgga accggcaaag gctttgcgaa ataactcacg gtagcctggt gaaccctgaa
      361 gtttttccgc gatcacggaa gcatcgccat gcatttcttc ggagttgttc aggacttctc
      421 ccgcctgatt ttcgggattc actacgcgca ggtcgaagaa caggctttgc tgcagcattg
      481 cattgatgag ggtgggggcg ttgcggcgta aaaccgagcc atgctgaaca ccgtgactgg
      541 tgagttgtcc atccgtaaag gctttatcgg gctggtggca actcgcgcaa ctgcgttcat
      601 tgttggatga aagcgccgga tcgaaaaaca gcatacgacc gattgccgtc agcagttcgt
      661 ggtcgccttc gtgctttgcc ggcatgaact tgtccagcag gaaagcgttg cggtcgaaca
      721 ccgatcgaac agccgggttg accgggtcag tcgaatccca gtcgggaaag cgggtgatga
      781 ggtagcggga aaacgcgtcg ctcattggaa ggaagcattg cgtatagagt gtatagtagt
      841 cgggctccga atctttgccg gatgatcgaa gtgtccgcgc tgcagtggcg agttggtcga
      901 cgaaattctt cagggcaagc cgggaacttt cgaattccgg ttgggtcaaa agcgaaagtc
      961 gtcttgcctg ttcattcagc gttcgtgcgg cttcttcgaa cgcgatccgg ctgaccggcg
     1021 tttccatctg ggtgaggccg gtgagaaaca gtcgcaagag ttggcggtgg cataggcgca
     1081 ggtagcttcg ctcgctggaa tattcattcg tcgcatgcag tttcaagcgc tgcagcagga
     1141 tcctgatcga ctcgaattgt gcctgcaact cttttcgacg ttgcggtccg gctgaatcga
     1201 aaagcgcagg ttcgagtgcc tgtaggcctg ccggtggcaa gatgggtgca aacgcctcct
     1261 cttcttccgg acgctcgagc ggggggccgt tccagcgttt gaaaagtttc cggtcggtta
     1321 acaacaacca ttcttccagc aacctgaaat ggactcgaac gtcgatccat cgttcccgaa
     1381 gtaggctatc ccggatcggg tcgtcagatc gaagggcagt cttacgaagt tcggccaggg
     1441 atttctccaa agccatgacg tcactgttgc gcagcgatac gagggtttgc gtatcgaact
     1501 ccggcaggga ggaagatgga ggttgccaac tgaatcccat caagaggatc agtaggacgg
     1561 gtattagcca ggtaaaacga aactgcatag gattcccgct tgaatttagg gaaatgaatc
     1621 cgatgaagaa gggtgaaaag tcattcgaag gaatcagcct tgtccgtgaa aatagcacca
     1681 agggctgtcg gtctgtaaac aactggtaat tagcacccta gaactggctt ggcccggcta
     1741 aacggaacaa aaggttagcc ccggagtttt atctttgcga gattaccccg aagtatgaaa
     1801 aagccgaccg tttacgttcc ccagaataag atccggattg ttaccgctgc ctcgcttttt
     1861 gacgggcacg atgccgctat caacatcatg cgccggatca ttcaaagcag tggcgcggaa
     1921 gtgattcacc tgggtcacga tcgttcggtt caggaagtgg tcgattgcgc gatccaggag
     1981 gacgtgcagg cgatcgccat gaccagctat cagggcggac atattgagta cttcaagtac
     2041 atgtatgacc tgttgaagga aaagggctgc ggacacatca agattttcgg tgggggcggc
     2101 ggtgtcattc ttccggaaga gatcagtgaa ctgcatgcat acggcatcac ccggatctat
     2161 tctccggacg acgggcgctc gatgggcttg cagggcatga tcaacgacct gctgaagcag
     2221 tgtgatttcc cgaccggcat caacctgaac ggagaagtga agcatttggc tgatcgtgag
     2281 ccgcgcgtgg tggcgaaact gatctcctgt gccgagaata atccggacga gtttagcaag
     2341 gttcatcggg atctcgataa actcgtcggc tcgaagaagg tgccggtact gggcatcacc
     2401 ggaacgggcg gagcaggaaa atcgtcgctg gtggatgaac tggttcgacg atttctcatc
     2461 gacttttcgg ataaaaccat cgccatcgtt tcggtggatc cttccaagcg caagaccggc
     2521 ggtgccctgc tgggcgatcg tatccggatg aattccattc atcacccgcg cgtgtatatg
     2581 cgttcactgg ccacccgcca gtcgaatctg gccttgtcga aatacgtcaa ggacgcagtc
     2641 gacatcctga aggcggccca atacgatctc attatcctgg agacatccgg catcggacaa
     2701 agcgataccg agatcctgga tcattcggat gtttccttgt acgtgatgac accggagtat
     2761 ggcgccgcat cgcagttgga aaagatcgat atgctcgact tcgccgacgt gatcgcgttg
     2821 aacaagttcg acaagcgcgg cgcgctcgac gcgttgcgcg acgtcaagaa gcaatatcag
     2881 cggaaccatc agttgtggga ccaggatccg gaagcgatgc cggttttcgg gacgatcgct
     2941 tcgcagttca acgatccggg aatgaaccgg ctttaccgca agctgatcga caagttgaac
     3001 gaaaaactgg aagcgggatt cgagagcaag ttcgagatca ccggagagat gtcggagaag
     3061 atttacatca ttcctccggc acgaacccgt tacctgagcg agatcagcga atcgatccgc
     3121 aactacaatc aatgggtgaa agaccaggcg gaaatcgcgc agcggctgtt tgccatccgg
     3181 aaatcactgg aaaccctgga tgcttcggat ctcgcggaga aggctcccgt taaattggcg
     3241 cttgacaagc tgtacgagca gaccgagttg aagctcgacg gacagaacaa gaaagcactg
     3301 caggaatggc cccggaaggt ccagcagttc cgggatgaag tctataccta caaagtccgc
     3361 gacaaggaag tcaaggtgaa gacttttacc gaatccctgt cgaggacgca cattcccaag
     3421 atcgccacgc cgaaatttga agcctggggt gaagtgttgc gttggagcct gcaggagaat
     3481 tttccgggcg agtttccttt taccgcgggg gtgtacccct tcaagcggac ggaagaagat
     3541 cccacgcgca tgttcgccgg cgaaggcgga cctgagcgca ccaaccgtcg tttccattat
     3601 gtgagcaagg gaatgcctgc caagcggttg tcgacagcat tcgattcggt aaccctttac
     3661 ggtcacgatc ctgattatcg cccggatatt tacggtaaga tcggtaacag cggcgtgtcc
     3721 atctgttgcc tggatgatgc gaagaagctg tacagcggat ttaatcttgc cgatccgaag
     3781 acatcggtat cgatgaccat taacggtcct gctgcgatcc tcaccggctt tttcatgaac
     3841 gccgcgatcg atcagcagtg cgaactgtac atccggcagc acggacttga gcaggaagtg
     3901 gaggcgaaga tccaggcgat ctacgcggca accggtacgg ctcgaccggc ttaccagggg
     3961 ccgttgcccg aaggtaacga cggtctgggc ctgatgctgc tgggcgtgac gggtgaccag
     4021 gtcttgccga aggaagtgta cgataagatc aaggcccaca ccgtgcaagt cgtgcgcggt
     4081 acggtgcagg cggatattct caaagaagac caggcgcaga atacctgtat tttctcgaca
     4141 gaattttcgc ttcgcctcat gggcgacgtg caacagtatt ttatcgagaa aaaagtgcgg
     4201 aatttttatt ccgtttccat atccggctac cacattgccg aagcgggcgc caatccgatc
     4261 acccaactgg ccttcacgct cgcgaatgga ttcacctatg tggagtatta ccgttcgcgc
     4321 gggatggaca tcaacgcgtt cgcgccgaac ctgagtttct ttttcagcaa cgggatcgat
     4381 ccagaatacg cggtgatcgg tcgggttgca cgcaggatct gggcgaaggc gatgaagcag
     4441 aagtacgcat ccgacgagcg atcgcagttg ctcaagtatc acattcagac ttccggccgc
     4501 tctttgcatg cgcaggagat cgacttcaac gacatccgga ccacgctgca ggccttgtat
     4561 gccatctatg acaactgcaa cagccttcat accaacgcct acgacgaagc catcacgacg
     4621 cctacggagg aaagcgttcg tcgggccatg gcgatccagc tgatcatcaa taaagaactg
     4681 ggcttggcca agaatgagaa cccgatgcag ggttcgttca tcatcgaaga gttgacggat
     4741 ctcgtcgagg aggcggtgat ggccgagttc gatcgcatca cggaacgtgg cggcgtcttg
     4801 ggcgctatgg aaaccatgta ccagcgcggc aagatccagg aggagagtct ttactatgag
     4861 accctgaagc ataccggcga attcccgatc atcggcgtga acaccttcct gtcctcgaaa
     4921 ggatcgccga ccgtcatacc ggctgaagtg atccgggcaa cgacggaaga gaaggagtac
     4981 cagatcagca tgttgaaaga cctccatgct cggaatgaga aaaaagcggt cgatcaattg
     5041 aagcaattg
//
LOCUS       DAOTON010000084         5001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13908_length_5001_cov_3.156490, whole genome shotgun sequence.
ACCESSION   DAOTON010000084 DAOTON010000000
VERSION     DAOTON010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13908_length_5001_cov_3.156490"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="P5292_02650"
     CDS             complement(<1..263)
                     /locus_tag="P5292_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRS38055.1"
                     /translation="MGSEKLVIAIDGFSSCGKSTLAKALARHLGYRYIDSGAMYRAVT
                     LFMIQHDLTVAQLESMNAEEMDRFLDHIKITFQVNPETKLSEV"
     gene            complement(528..1589)
                     /gene="porQ"
                     /locus_tag="P5292_02655"
     CDS             complement(528..1589)
                     /gene="porQ"
                     /locus_tag="P5292_02655"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HRS38056.1"
                     /translation="MHRTIRSLFLPAILLTSLAAHAQIGGNYVYQFLNVPASARIAAL
                     GGTLITVKDHDLNTALQAPSLINPSMDRSLALSGVLYPDGVKFGNAAFARDYGKLGTF
                     LAYMHYANYGEFRETNVFGDQEGTFRAADYALNLGWGYQYNPLFSVGAGMKFIYSDYY
                     LYNSFGIGVDISATYLDTVNNWTATILARNAGAQIDYYVKGNAEPLPIELLLGVSKRL
                     KHTPLRFNITYRHLQKFDLSYNDPFDLSGVDPLTGEAQQKKINFWNKLSRHFIIGTEI
                     LLSRNFHLRAAYNFQRRRELVVDTAPGTVGFSFGFGLKISKFVLGYGRGNFHQAGGAD
                     HFSITMNLAEFSKKAGDTN"
     gene            complement(1662..2603)
                     /locus_tag="P5292_02660"
     CDS             complement(1662..2603)
                     /locus_tag="P5292_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS38057.1"
                     /translation="MIPFVSPSSRQTLRLEGDFLVSPSGERFPVINGIPRFVPAENYA
                     DAFGFQWKNFARTQLDSHSGTPITRIRLERCLGFPINQLRGKEVLEVGSGAGRFTELL
                     LSAGAQVHSVDLSNAVEVNRENMGADKVYQLAQASVYELPFPPKSFDVVICLGVIQHT
                     PSSEQTIAALYAMAKPGGLLVIDHYPWRINYYFNPATWYRQVLKHLRPTTSKKIVDGM
                     VNFFFPWHWRFRNSKTLTWLLKRVSPLITFMNVFPELSKVQQEEWARLDTYDSLTDYY
                     KHLRTPDQIRKTLEALGGRELWVERGGNGVEARCTVG"
     gene            complement(2689..3177)
                     /locus_tag="P5292_02665"
     CDS             complement(2689..3177)
                     /locus_tag="P5292_02665"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRS38058.1"
                     /translation="MTIAQKYLEELERESAATRNILQAVPLDKSDWKPHPKSMSLGRL
                     ATHVAEIPSWFKNTLEDDELDFQKSDYKPYVARDTADLLGILEKNLAAAKGILEKFPD
                     ERMTDNWTMRNGDTIYFILPKEQVARTWCLNHWYHHRAQFGVFLRLLDIPLPGTYGPS
                     GG"
     gene            3669..>5001
                     /locus_tag="P5292_02670"
     CDS             3669..>5001
                     /locus_tag="P5292_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38059.1"
                     /translation="MNEVIIRPEAGAGPILLHSFSSVLTFSGVQFVTLDGRPGGNGTQ
                     SGLTLLCNGAGSAAVFTNGAYANWIRYCNLEASNSGSTSGVVSFTTTNSSLGNNNNWI
                     SDCYISGRGTSLNGIYSKGTAGKPNQFGRIENSHITGFLAKGICFEENTRHWQIINND
                     IYQESSVVFQFGPIHGIFVNDTLSDGFTITGNHIGGQAPACGGNPYNVVGSFIGVLMA
                     TGTDNLTIIDNNEVANIHWHNQFSLEYLAGIQVLAGKVQVGGPSGNLIGSLNRNGSIV
                     TSSYYLTEPEISGILIGYEILPGTIDTVRVLNNRIGGIRCETDSNNYLPTTLRGIYSF
                     QQKAGWIEVRDNHIGSPNLQNSLVSEGTTGDVIGIHLDVAYLPGNVLTFDFHNRIEGN
                     EITNLGSYNKGIQLRGGAPELTNNHIHHFYSNYVTGSTTIPNNTGINAIELR"
BASE COUNT         1263 a         1295 c         1253 g         1190 t
ORIGIN      
        1 tgaacttcgg aaagtttcgt ttcaggattg acctgaaaag tgatcttgat gtgatcgaga
       61 aaacgatcca tctcttcggc attcatcgat tccaactggg cgaccgtaag gtcgtgttga
      121 atcatgaata gcgtcactgc ccggtacatg gcgccggagt cgatgtatcg ataccccaaa
      181 tgccgggcta atgccttggc cagggtgctt tttccgcagg aggaaaaacc atcgatcgcg
      241 ataactagct tttctgagcc cacttcaggt tctgaacgga tggtataccc cccatttcct
      301 tttaacggat aggcagtcat gtttcggaag gcaatttagg aaaatggacg atgtcaaatg
      361 gctgcaatgc cggtcaatcc ctgcttcgcc ctgaaaacga attcactgaa ttttcgtgcg
      421 aagattgatt tcaattggat gagcaggaaa cctggcaaca tacagccatc caaccatcaa
      481 actaatatcg gcaaagtcaa aggaacgaag agcctgggcc tggaaaatca gttagtatct
      541 ccggcttttt tcgaaaattc agccaggttc atggtaatcg agaagtggtc ggcgcctccg
      601 gcctgatgga aattaccgcg tccatacccc agtacgaact tggatatctt caggccgaag
      661 ccgaacgaaa agccgaccgt tccgggcgcg gtatcaacta cgagctcgcg tctccgttgg
      721 aaattataag cggcacggag gtgaaaattc ctggagagca ggatctcggt accgatgatg
      781 aaatgacggg agagtttgtt ccaaaagttg atcttcttct gctgtgcctc gccggtcagt
      841 ggatcaacac cggataaatc aaagggatca ttgtacgaaa gatcgaactt ctgaagatgc
      901 cgataagtga tattgaaccg aagcggcgta tgctttaacc ttttcgaaac tcccagcaac
      961 aattcgatcg gcaggggttc cgcattaccc tttacataat agtcgatctg cgcgccggca
     1021 ttccgggcca aaattgtggc tgtccagttg ttgacggtgt ccagatacgt ggcgctgatg
     1081 tccacaccga ttccgaagga attatagagg taataatcgc tgtaaatgaa tttcattccg
     1141 gcccctacgc tgaacaaggg attgtattgg taaccccatc cgaggttcag cgcgtagtcc
     1201 gctgcccgga acgtaccctc ctgatcgcca aacacgtttg tctcccggaa ctccccgtag
     1261 ttggcgtaat gcatgtaggc gaggaatgtt cccagcttac cgtaatccct ggcaaaagcg
     1321 gcgttgccga atttcacgcc atcgggatac aagacaccgc tcaacgcgag tgaacggtcc
     1381 atggaaggat tgatgaggga cggcgcctgc agggcggtat tcaggtcgtg gtccttcacg
     1441 gtaatcaatg ttccgcccag cgcggcgata cgggccgaag ccggtacgtt caggaactgg
     1501 tagacataat tacctccgat ctgtgcatgg gcagcaagcg aagtgaggag aattgccggc
     1561 aggaagagcg atcggatggt ccggtgcata cgaggtaaat gtaggaaatt gaacaggaac
     1621 gattcagcga ccggaatatt gcacacagca ggtactgttt ttcatcctac ggtgcaacgg
     1681 gcttccactc cattgcctcc ccgttccacc cacagttcgc ggcctcccag cgcttcaagt
     1741 gttttcctga tctgatcggg tgtccggagg tgcttgtagt agtccgtcag ggaatcgtac
     1801 gtgtccaacc gtgcccattc ttcctgctgc actttgctca gttccggaaa aacattcatg
     1861 aacgtgatga gcggtgaaac gcgtttcaac agccaggtca gggtcttgct attccgaaac
     1921 ctccaatgcc agggaaagaa aaagttcacc attccgtcca cgatcttttt ggaagtggtc
     1981 ggacggaggt gcttcaacac ctggcggtac caggtagctg gattgaaata atagttgatg
     2041 cgccagggat aatggtcgat caccagtagc ccacccggct tagccatcgc atacaaagca
     2101 gcgatcgtct gctccgaact gggcgtatgc tgaatgaccc ccaggcagat caccacatca
     2161 aagctcttcg gcggaaaggg cagttcatag acacttgcct gcgccaattg atagactttg
     2221 tcggctccca tgttttcccg gttgacctcg acagcattgc tcagatcgac cgaatgcacc
     2281 tgtgctcctg ccgagagcaa taactccgta aagcgacctg ctccgctgcc gacttccagc
     2341 acttcctttc cacggagctg attaatggga aaccccagac aacgctccag tcggatcctg
     2401 gtgatcggcg ttccgctatg actgtctaat tgggtgcgcg caaagttctt ccattgaaag
     2461 ccgaaggcat cggcatagtt ctcggccggt acgaagcggg gaattccatt aataacaggg
     2521 aatcgctccc cggagggaga aaccagaaag tccccttcaa gacgaagcgt ttgtcgactg
     2581 gaaggtgaaa caaaaggaat catacacgta cgcgaatagc ggggcgaatg tagggaattt
     2641 caggccgagc ttagcctgcc gtaggtcccc ggcactcgcc ctgatcgctt aaccgccgga
     2701 gggaccgtac gtacccggca agggtatgtc cagcaaacgc aagaacactc caaactgggc
     2761 gcgatgatga taccagtgat tcaggcacca ggttctggcg acctgttctt tgggcagtat
     2821 gaagtaaatg gtatcgccgt tacgcattgt ccagttgtcg gtcatccgtt catccggaaa
     2881 cttttccagg atacctttag cggctgccag gtttttttcg agtatgccga gcaaatcggc
     2941 agtatcccgg gctacatagg gcttgtaatc actcttttga aaatcgagtt catcgtcctc
     3001 cagggtattt ttaaaccagg acggaatctc cgccacatgc gtagccagtc gcccgaggct
     3061 catgctcttc ggatgaggtt tccagtccga tttatccaag gggactgcct gcaagatatt
     3121 tcgggttgcg gctgactctc tttccaattc ttccagatat ttctgtgcaa tagtcatgat
     3181 tgtgatactg tcgttggtga cactgtcaat aaatgaaatt attcataatg caggaaattt
     3241 ctttcgcatc attttccaaa gtgattggaa agcaatcaat gcacgagcat caaaaaaccg
     3301 atccgggtgc ttctacctga tttgataggc catgagcatt caaatgttcc tggactttcg
     3361 gttaatttgc caggccaaaa cattgttccc tccatcttat gcatcttcaa tcagcatcat
     3421 cgaccctgca gcgactttta acccaatttc tgttgctgct tccaacgatg gtattttcac
     3481 aaccctgcaa tttacaagga acctataaaa tcggtcctgg gggacagtat ccgagtctga
     3541 cgaacgccat cgccgaccta atgagtaccg gagttggcgg gaccgttact cttgaattca
     3601 aagcaaacta cactgattcg gttgaaacct tcccgatttt tatcggaaac atcccctgtg
     3661 tttcggcagt gaacgaagta ataatccggc cggaagcagg tgcagggcca atactcttgc
     3721 attcattcag cagtgtcctg accttttccg gcgtccaatt tgtcacgctg gatggtcgac
     3781 ccggaggcaa cggaacgcaa tccggactca cccttctgtg caatggtgcc ggcagcgcgg
     3841 ctgtctttac caatggcgcg tatgccaact ggattcgtta ctgcaatctg gaagcttcca
     3901 attcgggatc caccagcggg gtggtttcat ttaccacaac aaacagttcc cttgggaaca
     3961 acaataattg gatcagtgat tgttacatca gcggtcgcgg gaccagctta aatgggatct
     4021 attccaaagg gaccgcgggc aagccgaatc aattcggacg tattgaaaac tcccacatca
     4081 caggattcct ggccaaaggc atctgtttcg aggagaacac gcgtcactgg cagatcatca
     4141 acaatgacat ttaccaggaa agttctgtcg tctttcaatt tggaccgatt catggcatct
     4201 tcgtaaacga tacgctcagt gacggtttta cgattacagg aaatcacatt ggcgggcagg
     4261 cacccgcctg cgggggaaac ccctacaatg tcgttggcag cttcattggc gtactcatgg
     4321 ccacgggtac agacaatctc actatcatcg acaataacga agtagccaac atccattggc
     4381 ataatcagtt ttcacttgaa tacctagccg gcatccaggt actggcaggc aaggtccagg
     4441 ttggcggccc ttccggcaac ctgatcggca gtctaaatcg gaacggcagt atcgtaacaa
     4501 gctcctacta tcttacagaa cccgagatct ccggcatcct gatcggatat gagatattgc
     4561 cagggacgat cgacacggtg cgggtgctga ataaccgtat cggcgggatc cgttgcgaaa
     4621 ccgattccaa caattatctc cccaccaccc tgcgtggcat ctattccttc cagcagaaag
     4681 cgggctggat cgaggtccgg gacaaccaca tcggaagtcc gaacctgcag aacagtctgg
     4741 tgtcggaagg cacaaccggc gatgtgatcg gcatccacct ggatgtagcc taccttcctg
     4801 gtaacgttct aacctttgat ttccacaacc ggatcgaagg gaatgaaatc acgaacctgg
     4861 gcagttacaa caaaggaatt cagttacggg gtggcgctcc tgaattgacg aataatcaca
     4921 tccatcattt ttactccaat tatgtaacgg gttcaactac cattccgaac aataccggca
     4981 tcaacgcgat cgaattgcgg c
//
LOCUS       DAOTON010000085         4996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13925_length_4996_cov_3.323214, whole genome shotgun sequence.
ACCESSION   DAOTON010000085 DAOTON010000000
VERSION     DAOTON010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13925_length_4996_cov_3.323214"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2541
                     /locus_tag="P5292_02675"
     CDS             <1..2541
                     /locus_tag="P5292_02675"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRS38060.1"
                     /translation="PTAISLILFNTYFSGMIPTFPGLRLNVRLLLSQLLVLGSIASTA
                     QITRVNGRVYDPLTNEPIPFASILFVGTTVGKNADLDGNFSIETTQPVDSIRATFVGY
                     LPATIHVKRGQTQKIDFALRANKFDLPEVTIVAGENPAIILLRKVIDNKPKNDPDRFS
                     ALQYETYNKLEFDINNITDKFKRRKLFKPFSFVFDNIDTTSTNKKPYLPVFLTESVSD
                     VYQRKNPKLKKEVIRASKISGVENATVSQYLGDLYAKINVYDAYLYLFGKGFVSPISG
                     ISQLYYKYMLVDSAYLDNKWCYKITFHPRRKQELTFNGEFWVHDTTFAIRKLNMRISG
                     DANINFIEDLAIVHDYTFVADQTWLLAKEVLVVNLAPTETKSKETTGFIGRKTTFYRN
                     FKLNDPQPESFFAEGHEVIVKDDAYKQTSAFWDSTRGESLSESERKIYAMIDTIKTIP
                     AYKRWADLVRVAATGYKEVGLFEIGPIYKFISSNPIEGFRLRFGGQTSNKFSTRLVLS
                     GYSAYGFKDERFKYGGTLRYFTSKDPRQYFGVEYTKDVEQLGVSANAFANDNVFATLL
                     RRQQALLLNDVEMEKVYFEHEWKPGFSNRVSITHTEYAPLGKLNFTYFANDAKTDSSS
                     RINNTDLSFYIRFANHERFVSGEVDRISLGTNWPIFHLQYQTGLKDFLESDFTYHKLR
                     FKVEDYLYPGNFGTFYFTAEAGKIWGTLPYPLLYIHPGNNSFVYDRSAFNLMNFYEFV
                     SDEYISFKAEHNFGGFFLDRIPALRKLKWREMATFSAVTGKVSDRNRDILSRPDAFYS
                     LGKPYIEAGVGISNIFRILRIDNIWRLSYLDHPDIAKVAILGTVHISF"
     gene            2587..3312
                     /gene="lptB"
                     /locus_tag="P5292_02680"
     CDS             2587..3312
                     /gene="lptB"
                     /locus_tag="P5292_02680"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRS38061.1"
                     /translation="MILRADQLIKRYKQRTVVDRVSIQVEQGEIVGLLGPNGAGKTTT
                     FYMTVGLIQPNEGRIFLDQTEITGLPMYKRAQLGIGYLAQEASVFRKLSVEDNIKAVL
                     EMTRLSKEEQSRKLESLLDEFGLQRIRGNRGDLLSGGERRRTEIARALAVDPKFILLD
                     EPFAGVDPIAVEDIQGIVAHLKDRNIGVLITDHNVHETLSITDRAYLLFEGRILKAGT
                     AEELAADEQVRRVYLGQNFVLRK"
     gene            complement(3343..3597)
                     /gene="purS"
                     /locus_tag="P5292_02685"
     CDS             complement(3343..3597)
                     /gene="purS"
                     /locus_tag="P5292_02685"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRS38062.1"
                     /translation="MKFIAEIDVMPLKALLDPQGKAVTHGLQNMGYATMENVRVGKHI
                     SLEIDADSREAANQQVEEACKKLLANPIMESFHFVLNEAK"
     gene            complement(3594..4313)
                     /locus_tag="P5292_02690"
     CDS             complement(3594..4313)
                     /locus_tag="P5292_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRS38063.1"
                     /translation="MKKHLPNLLTSLNLFCGCIASILVFRGKVEWVGYVVLLAAVFDL
                     FDGFLARKLGVSGPFGRELDSLADMVTFGFVPGAILFQLLQASDLALAFPDPDLRRVI
                     QFLPFVVTVFSGLRLAKFNIDTRQSTSFIGLPTPANTLWIISLPIILTWYPGQYEFLR
                     SHWVILLLSILSAGLLVAELPLFSLKFKHFGWKGNEYPYILILIALPLALFFSFAALP
                     MIVATVVLLSLVRNLIPRTAP"
     gene            4592..>4996
                     /locus_tag="P5292_02695"
     CDS             4592..>4996
                     /locus_tag="P5292_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38064.1"
                     /translation="MNRTLLTAYCLLGLFGIVNSQTPKYSNEFLSIGVGARPMGMSGA
                     VAASTDDATSAAWNPAGLNGIGSDIQIAAMHAELFAGISKFDYGTVATRIDTNRVIGF
                     SVIRFGTDDIPNTLDLMDASGVIDYSKLKSFSI"
BASE COUNT         1278 a         1417 c         1236 g         1065 t
ORIGIN      
        1 ccaactgcaa tcagcctgat ccttttcaac acgtattttt cgggcatgat tcccactttt
       61 ccgggcttac gattgaatgt acggctcctc ctctcccagc ttttggtcct gggaagcatt
      121 gcctccaccg cgcagatcac ccgcgtcaac ggacgggtct atgacccgct caccaacgaa
      181 ccgataccct ttgccagcat cctcttcgtc gggacaaccg ttggtaagaa tgccgacctc
      241 gacggcaact tctcaatcga gaccactcag ccggtcgaca gcattcgagc cactttcgtc
      301 ggctacctgc ccgctacgat ccatgtgaag cgaggccaga cccagaagat cgatttcgcg
      361 ctacgggcca acaagttcga ccttccggaa gtcaccatcg ttgccggaga gaaccccgcc
      421 atcatcctgc ttcgaaaggt catcgataac aagcccaaga acgaccccga ccgcttttcc
      481 gcgctccagt acgaaaccta taacaagctg gaattcgaca tcaacaacat caccgacaaa
      541 ttcaaacgtc gcaagctgtt caaacctttc tcgttcgtat tcgacaacat cgacacgacc
      601 tcaaccaaca agaaacccta tttacccgtc ttcctgaccg aatcggtctc ggatgtgtat
      661 caacgcaaga atccgaaact gaagaaggag gtcattcggg ccagcaagat ctcgggcgtc
      721 gagaacgcca ccgtttccca gtacctggga gacctgtacg ccaagatcaa cgtatacgat
      781 gcctacctct atcttttcgg aaagggcttc gtcagtccga tcagcggcat ttcccagctt
      841 tactataaat acatgctggt cgacagcgcc taccttgaca acaagtggtg ctataagatc
      901 acgtttcatc cccggcggaa acaggaactt accttcaacg gggaattctg ggttcacgac
      961 accacctttg ccatccggaa actgaacatg cgcatcagcg gtgacgcaaa catcaacttc
     1021 atcgaggacc tggccatcgt tcacgactac accttcgttg ccgaccaaac ctggttgttg
     1081 gcaaaggaag tcctcgtggt caatctggcg ccgacggaaa ccaaatcaaa agagaccacc
     1141 ggcttcatcg gacgtaaaac aaccttttac cggaacttca aattgaacga cccgcaaccg
     1201 gagagtttct ttgccgaagg acacgaggtg atcgtaaaag atgacgctta caagcagacc
     1261 tcagcttttt gggactccac ccgcggcgaa agcctgagtg aaagcgaacg gaagatctac
     1321 gcgatgatcg atacgatcaa aacgatcccg gcgtacaaac gctgggcgga cctggttcgc
     1381 gtggccgcca cgggctacaa ggaagtagga ctcttcgaaa tcggccctat ctacaaattc
     1441 atttcctcca atccgatcga agggtttcgc ctccgcttcg gcggccagac cagtaacaaa
     1501 ttcagcaccc gcctggtgct gagcggatat tccgcttacg gcttcaagga cgaacgattc
     1561 aaatacggcg gcaccttgcg ctacttcacc tccaaggacc cgcgtcagta cttcggcgtg
     1621 gaatacacaa aggatgtcga gcagctcggc gtgagcgcca acgcttttgc gaacgacaac
     1681 gtcttcgcga ctttactccg gcgtcaacaa gccttgttgt tgaacgatgt cgaaatggaa
     1741 aaagtctatt tcgaacacga atggaaaccc ggcttctcca accgcgtcag catcacccat
     1801 accgaatacg caccgctggg caaactcaac ttcacttact tcgccaatga cgcgaagacg
     1861 gacagcagca gccggatcaa caacaccgac ctttccttct acatccgctt cgccaaccac
     1921 gaacgtttcg tatcggggga agtggaccgc atctcccttg gaaccaactg gccgatcttt
     1981 cacttgcagt atcagaccgg actcaaggac ttcctggaaa gcgatttcac ttaccacaag
     2041 cttcggttca aagtagaaga ctatctgtat cccggaaact tcggcacctt ctacttcacc
     2101 gctgaagccg gaaagatctg gggcaccctg ccctatccgc tcctgtacat tcatcccgga
     2161 aacaattcat tcgtgtacga ccggtccgca tttaacctga tgaacttcta cgagttcgtc
     2221 agcgatgaat acatcagctt caaagccgag cacaacttcg gcggcttctt cctcgaccga
     2281 attccggcat tgcgtaaact gaaatggcgg gagatggcaa ccttcagcgc agtcaccgga
     2341 aaagtatcgg acagaaaccg ggacatcctt tcgcgccctg acgctttcta ttcgttgggt
     2401 aagccctaca tcgaagccgg ggtcggcatc tcgaacatct tccgcatcct gcgtatcgac
     2461 aacatctggc gtctatccta tctggatcat cccgacattg ccaaagtcgc gatcctgggc
     2521 accgttcaca tcagctttta aggttgggca tccgcttcgg attccccatc tttgcgcctg
     2581 tccagcatga tcctacgcgc cgatcaactc atcaagcggt ataaacaacg caccgtcgtt
     2641 gaccgtgttt ccattcaggt cgaacaaggc gaaatcgtcg gtttgctcgg gcccaatggc
     2701 gccgggaaaa cgactacttt ttacatgacc gtaggcttga tccagcccaa cgaagggcgg
     2761 atcttcctgg atcagacgga gatcacgggc ttgccgatgt acaagcgtgc tcaactgggt
     2821 atcggctacc tggctcagga ggcctccgtt ttccgcaaac tgagtgtgga ggacaatatc
     2881 aaagccgtgc tcgaaatgac ccggctgagc aaggaagaac aatccagaaa gctggaatcc
     2941 ctgctcgacg aattcgggct gcagcgtatc cgcgggaacc gaggcgatct gttatcgggt
     3001 ggcgagcggc gcagaaccga gatcgcacgc gccctggcgg ttgatccgaa attcatcttg
     3061 ctcgacgaac ccttcgctgg cgtggacccg atcgcagtgg aagacatcca gggaatcgtc
     3121 gctcacctga aagaccggaa catcggcgtg ttgatcacgg accacaacgt tcacgaaaca
     3181 ctttcgatca ccgatcgggc ttacctcttg tttgagggaa gaattctaaa agcaggtaca
     3241 gcggaagaac tggcagccga cgaacaggtc cgccgggttt acctcggtca gaattttgtc
     3301 ctgcgaaaat aggtaacggc tatttcggca gcatgccgtt tatcacttcg cctcgttgag
     3361 gacgaaatga aaactctcca tgatcggatt cgccagcagt tttttacacg cctcttccac
     3421 ctgctgattg gccgcttcgc gggaatccgc atcgatctcc agcgaaatgt gtttgccgac
     3481 gcgcacattc tccatggtcg cgtagcccat attttgaagg ccgtgcgtca ccgcttttcc
     3541 ctgcggatcc aataaggctt tcaatggcat aacgtcgatt tcggcaataa acttcatggt
     3601 gcggtgcggg gtatgaggtt tcgtacgagc gacaagagta cgacggtggc gacgatcatg
     3661 ggcaatgccg cgaaggaaaa aaagagggcc agcggcaaag cgatcaggat caggatgtac
     3721 ggatattcat ttcccttcca gccaaaatgc ttgaacttga gtgagaacag cggcaactca
     3781 gcaaccagga gtccggcaga caggatggac aacagaagaa tgacccaatg ggatcgcagg
     3841 aactcgtact ggccgggata ccaggtcagg atgatcggaa gcgaaatgat ccaaagtgta
     3901 ttggccggag tcgggagccc gatgaaggaa gtcgattgcc gggtatcgat gttgaatttc
     3961 gccaggcgga gaccggagaa gacggttacg acgaacggta gaaactgaat cacacgacgg
     4021 aggtcaggat cagggaaagc cagggcaagg tcggaggctt gcaggagttg aaagaggatc
     4081 gctcccggga cgaacccgaa ggtcaccata tcggccaggg agtccagttc ccttccgaag
     4141 ggtccgctaa caccgagttt tcgcgccaga aaaccatcga agagatcaaa tacggccgca
     4201 agcaggacga cataacccac ccactctacc ttgccccgga aaaccaatat ggaggcgatg
     4261 caaccacaaa aaaggttgag ggaagtcagg aggttgggca agtgcttttt caagggacaa
     4321 agccggcggg gcgtgtaaaa gtaatgattg aataaaaagg gataaatcca tgcggccatc
     4381 ttacagtttt aatgtgtcag attgtcgaag gaattccgtc aacccccttg ttggaaaccc
     4441 ggtataacag tattgataaa tgcttgaaat cttccaaaaa accgacttca tctgcttgca
     4501 taaatcgtaa ctttactata ggtaacctgt tgattatcta taataaaacc ttaccaaaag
     4561 gcgttttgcc cggatgaact tgctccgctc catgaatcgt acgcttctga ccgcttattg
     4621 cctgcttggc ctcttcggga tagtcaattc ccagactccg aagtacagta atgaattcct
     4681 gtccatcggc gtaggtgcac ggcccatggg catgtccggt gccgtggctg ccagcaccga
     4741 cgacgccacc tcggctgcat ggaatccggc cggcctgaac gggatcggca gcgacattca
     4801 gatcgcggcc atgcacgccg agctcttcgc gggtatttca aaattcgatt acggcactgt
     4861 cgctacacgc atcgacacga atcgagtcat tggcttttcc gtgatccgat tcggaacgga
     4921 tgacatcccc aacaccctgg acctcatgga tgcatccggt gtgatcgatt attccaaatt
     4981 gaaatccttc tcgatc
//
LOCUS       DAOTON010000086         4995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13931_length_4995_cov_4.138664, whole genome shotgun sequence.
ACCESSION   DAOTON010000086 DAOTON010000000
VERSION     DAOTON010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13931_length_4995_cov_4.138664"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2184
                     /locus_tag="P5292_02700"
     CDS             <1..2184
                     /locus_tag="P5292_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRS38065.1"
                     /translation="HARFRALELMSVRQPISVNLPSGKVSDYYGQHVFSQDAMQRFLS
                     KDAYRAVMDAINHGGMIDRKMAGQIALGMKEWASGKGATHYTHWFQPLTGLTAEKHDS
                     FFELNDGRAIELFSGNALVQQEPDASSFPSGGIRNTFEARGYTAWDPSSPAFIMERAG
                     GKTLCIPTVFVSYTGETLDYKAPLLKALHALDKAAVDVCQYFDKDVTKVMATLGIEQE
                     YFLVDAALLQSRPDLAITGRTLFGHSPAKGQQLEDHYFGSIPERVLGFMLDLETEAYK
                     LGIPLKTRHNEVAPSQFECAPIFEDINLAVDHNCLLMDVMDKIARRHGFHILLHEKPY
                     AGINGSGKHNNWSLGTNTGKNLLSPGSTPKSNLMFLTFFINTIRAVFEHADLLRASIA
                     SASNDHRLGANEAPPAIMSIFLGGQLSKVLDDLEKSVSEKKMTPDEKTELKLNIGKIP
                     EILLDNTDRNRTSPFAFTGNKFEFRAVGSSANCSNAMTVLNTIVASQLAAFKKEVDGL
                     IEKGTKKDEAILKVLRRYIVESKNIRFEGNGYSDEWVKEAKRRGLSNVTTTPPALDAY
                     ISKKSLKLFEEVGIFSHREAEARHEIMLETYIKKIQIEARVIGDLAINHILPAAVKYQ
                     TILAENITALKSAGMKADTYSMQLEQLKEISGYIASIKSNVDAMVEARKRANKLENVR
                     DKAIAYCDKVKAYFDPIRDDVDHLEMLIDDALWPMAKYRELVTIR"
     gene            complement(2260..4362)
                     /locus_tag="P5292_02705"
     CDS             complement(2260..4362)
                     /locus_tag="P5292_02705"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRS38066.1"
                     /translation="MNFQNATAAGELLQTEQSSATTNPLMEKWTGNYGGVPPLDKIQV
                     AHFKPALEAAMKENLAEIDKITANAAAPSFDNTIVEMERSGKLLDRVQTIYGIWSSTM
                     STKDFQGVEEEMEPKLAAFYDQIVQNEKLFQRIEKVYTDEAAMKALTPEQKRLTWNYY
                     TNFTRAGAKLDAKSKARLSEINQELASLFTKFSQNQLHDEMEIRLVLTSKEDLAGLPQ
                     SLVDAAAAAAKEPGTWVIRNTRSSVEPFLTYSSRRDLREKAWRMFVNRGDNGGEFDNN
                     SIITNILKLRAERAKLLGYSTHAHWRLENSMAKTPERAVELMEKVWTPAVARVKEEVA
                     DMQAQAEKEGDKITIEPWDYRYYAEKVRKAKYDLDQDEVKQYCQLEKLREGMFWVAGE
                     LFDFEFTPVTDVPVNHPDVRVWQVKEKTSGKAIGLWYFDPYAREGKRSGAWMNAYRNQ
                     QRVDGEILTIVSNNSNFVKGKPGEPVLISWDDANTLFHEFGHALHGLCSNVTYPSLSG
                     TNVVRDYVEFPSQLLEHWFSTPEVLKRFALHHKTNEPIPGELVARIEKAATFNQGFIT
                     TEFLASALVDMKLHLEGGAEIDPDKFERVTLEKLGMPKEIVMRHRTPQFGHVFSSDGY
                     SAGYYSYLWADVITADAYEAFTEAGGPYDKSVGAKLKKEIFSVGNTLDPAEAYRNFRG
                     RDAKIDGLMRDRGFPLTN"
     gene            4660..>4995
                     /locus_tag="P5292_02710"
     CDS             4660..>4995
                     /locus_tag="P5292_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38067.1"
                     /translation="MKSSKKLIFTCLFAICSLFVSAQRGPTWQADQAWTKGDYYDAAL
                     LYKKAFTKEKNKAKKAEIIFKVGEAYRMVNDSKNQEVWYAKAIKANYKNPEVFLRYAD
                     ALKLNSKYDE"
BASE COUNT         1150 a         1389 c         1349 g         1107 t
ORIGIN      
        1 cacgcgcgct ttcgggccct ggagctgatg tctgttcgtc agccgatttc tgtgaacctg
       61 ccatccggca aagtgtccga ttattacggg caacacgtgt tcagccagga cgccatgcag
      121 cgattccttt ccaaggatgc gtaccgggct gtcatggatg ctatcaatca tggcggcatg
      181 atcgaccgta aaatggcggg ccagatcgcc ctgggcatga aagaatgggc gagcggaaaa
      241 ggcgccacgc attacacgca ttggttccag ccgctcaccg gcctgacggc cgaaaagcat
      301 gattccttct ttgaactgaa cgacggtcgg gccattgagt tgttctccgg caacgccctg
      361 gttcagcagg agccggatgc gtcgtccttc ccatcgggtg gcatccgcaa tacgttcgag
      421 gcacgaggtt acacggcctg ggacccgagc tcccctgcct ttatcatgga acgtgccggc
      481 ggtaagacgc tgtgcattcc gaccgttttc gtttcctaca cgggagagac actggactac
      541 aaagctccct tgctgaaagc cttgcacgcg ctcgacaaag ccgctgtcga tgtttgtcag
      601 tactttgaca aggatgtcac gaaagtcatg gccacgctgg gcattgaaca ggaatatttc
      661 ctggtcgatg ccgcgctcct gcagtcgcgt cccgacctgg ccattaccgg tcgcaccctg
      721 ttcggccatt ccccggcaaa aggccagcag ctcgaggacc actacttcgg atccattccg
      781 gagcgggtgc tcggtttcat gctggacctg gagacggagg cctataaact gggcattccg
      841 ctgaagaccc gccacaacga agttgcaccg agccagttcg aatgcgcgcc gatctttgaa
      901 gacatcaacc tggcggtgga ccacaactgc ctgttgatgg acgtcatgga caagatcgcc
      961 cgtcgtcacg gctttcacat cctgttacat gagaagccgt acgccggcat caacggttcc
     1021 ggcaagcaca acaactggag tcttggcacg aacacgggca agaacctgct ctctcccggc
     1081 agcacgccga agagcaacct gatgttcctg accttcttca tcaacacgat ccgcgcggta
     1141 ttcgaacacg ctgatctgtt gcgcgccagc atcgcctcgg ccagcaacga ccatcgtctc
     1201 ggcgccaacg aagctccgcc ggcgatcatg tcgatcttcc tcggcggtca gttgagcaaa
     1261 gtgctcgatg atctggaaaa gagcgtgtcg gagaagaaga tgacaccgga cgaaaagacc
     1321 gagttgaaac tgaacatcgg gaagatcccg gaaatcctgc tcgacaatac cgaccgtaac
     1381 cgtacttctc cttttgcttt caccggtaac aagttcgaat tccgggcagt cggctcctcc
     1441 gccaactgtt ccaacgcgat gacggtgctg aacaccatcg tcgcttccca gttggccgct
     1501 ttcaagaaag aagtcgacgg gctgatcgag aagggcacga agaaagacga agccatccta
     1561 aaggtattgc gccgttacat cgtcgagtcg aagaatatcc gcttcgaagg aaacggttac
     1621 agcgacgaat gggtgaaaga agcgaagcgc cgcggactct cgaacgtgac cacgacccca
     1681 cccgcactgg acgcgtacat ttccaagaag tcgctcaaac tgttcgagga agtcggcatc
     1741 ttttctcacc gcgaagcgga agcccgccac gagatcatgc tggagaccta tatcaagaag
     1801 atccagatcg aggcgcgggt tattggagat ctcgcgatca accacatcct gccggcggcg
     1861 gtgaagtacc agacgatcct ggctgagaac atcacggctt tgaaatcggc cggtatgaaa
     1921 gcggacacgt acagcatgca gctcgagcaa ctgaaggaga tctccggcta tatcgcctcg
     1981 atcaagtcga atgttgacgc gatggtggaa gcacggaaac gtgccaataa gctggagaac
     2041 gtccgcgaca aggcgatcgc gtattgcgac aaggtgaagg cctacttcga tccgatccgc
     2101 gacgatgtcg accatctcga aatgcttatc gacgacgcgc tgtggccgat ggcgaagtac
     2161 cgggaactgg tgaccattcg ttaacaacga atacaaaatg aaaaaaggcc tgtccgaatc
     2221 cggacaggcc tttttttctt ccttgcggaa atcattggat tagttcgtga gcggaaatcc
     2281 gcggtcgcgc atcagtccat cgatcttcgc gtcacgaccg cggaagttgc ggtatgcctc
     2341 tgcgggatca agggtgtttc ctacagagaa aatctccttc ttcaatttcg cgccaaccga
     2401 tttgtcatag ggcccaccgg cttcggtgaa cgcctcgtac gcatcggccg tgatcacatc
     2461 ggcccacagg tagctgtaat agccggcgga atagccgtcg ctggagaata cgtgaccgaa
     2521 ttggggagtg cggtgacgca tgacgatctc cttgggcatc ccgagctttt caagcgttac
     2581 acgctcgaat ttgtccggat cgatctccgc tccgccctcg aggtgcagtt tcatgtcgac
     2641 caacgcgctt gcgaggaatt cggtcgtgat aaagccctgg ttgaaggtgg cagccttttc
     2701 aatgcgtgcg acaagttcac ccggaatggg ctcgttggtc ttgtgatgca gggcaaaacg
     2761 cttgaggact tcgggagtcg agaaccagtg ctccagcaac tgggaaggga actctacgta
     2821 atcgcgtaca acattcgttc cggacaagga aggataggtt acgttcgagc agagaccatg
     2881 cagcgcgtgg ccgaactcat ggaacagggt gttggcatca tcccaggaga tcaataccgg
     2941 ctcaccgggt ttgcccttga cgaagttgga gttattcgat acgatggtca aaatctcccc
     3001 atcgacgcgt tgctggttgc ggtaggcgtt catccaggca cccgatcgtt ttccttcacg
     3061 ggcataggga tcaaagtacc aaaggccaat ggctttgccg gaggtctttt cttttacctg
     3121 ccatacgcgg acatccggat gattgaccgg aacatcggta accggggtga attcaaaatc
     3181 gaagagttca ccggcaaccc agaacattcc ttcgcggagt ttttccagtt ggcaatattg
     3241 cttcacctca tcctgatcca ggtcgtactt tgccttacgg accttttcag catagtagcg
     3301 atagtcccag ggttcgatcg tgatcttatc gccctctttc tccgcctgcg cctgcatatc
     3361 agctacttct tcctttaccc gggcaacggc cggcgtccag accttctcca tcagttcgac
     3421 cgcgcgctcc ggagttttag ccatggaatt ttcgaggcgc cagtgggcat gcgtcgaata
     3481 tccgagcagc ttggctcgct cggcgcgcag cttcagaatg ttggtgatga tcgaattgtt
     3541 atcgaactcg ccaccattgt cgccacggtt gacgaacata cgccaggcct tttcgcgcag
     3601 gtcgcgtcgg gaggaatacg tcagaaacgg ttcgaccgaa gaccgcgtgt tgcggatcac
     3661 ccaggtaccc ggctccttcg ctgcagccgc tgctgcgtcg acgagtgatt ggggcaagcc
     3721 ggccagatcc tccttgctgg tcagtaccag acgaatctcc atctcgtcgt ggagctgatt
     3781 ctgactgaac ttggtgaaga gcgaagcaag ttcctggttg atttccgaca gacgcgcttt
     3841 cgatttagcg tccagcttgg caccggcgcg ggtgaagttg gtgtagtagt tccaggtcag
     3901 acgcttctgt tcgggcgtca aagccttcat cgccgcttcg tcggtataaa ccttttcgat
     3961 ccgctggaaa agcttttcgt tctgcacgat ctggtcgtag aacgccgcga gcttcggctc
     4021 catctcctcc tctacaccct ggaaatcctt ggtcgacatg gtcgaactcc agataccgta
     4081 gatcgtctgc acacggtcca gcaattttcc ggaacgttcc atttcaacga tggtattgtc
     4141 aaacgatggc gctgcggcat tcgccgtgat cttgtcgatc tcggcaaggt tttccttcat
     4201 cgcggcttcc aaggccggct taaaatgggc aacctggatc ttatccaggg gcggaacccc
     4261 gccgtaattt cccgtccatt tctccatgag cgggttggtg gtagcggaac tttgctccgt
     4321 ttgcagcagc tcacctgccg cagtggcgtt ttggaagttc atcggaattg ccataaggca
     4381 gaggctgccg cccagcagaa gccggcggaa tgatcgtgtt ttgttttgca tggatggaag
     4441 taacatttcg tgtgaggtgt gaggacacat tgaccttgac caaaggtaaa caatcgtgtt
     4501 tcccacccca gttaaaggat gttaaacaaa acgtgggaaa cgtcgtagaa cgtcctcaaa
     4561 aacgagcgga acttccatat ttgccccctc gaaaattctt cctttctttg cttacacggg
     4621 aaaattccca accaatttca ggcaccaaat taccatccta tgaaatcaag caagaagctg
     4681 attttcacat gcttatttgc aatctgcagc ctctttgtca gcgcccagcg cggacccacc
     4741 tggcaagctg accaggcctg gaccaaaggc gactattacg acgctgcctt gttgtacaag
     4801 aaggctttca caaaggagaa gaacaaggca aagaaggccg agatcatttt caaggttggc
     4861 gaagcctacc ggatggtcaa cgacagcaag aatcaggagg tttggtacgc caaggcgatc
     4921 aaagccaatt acaagaatcc ggaggttttc ctgcgttatg ccgacgcctt gaaactcaac
     4981 agcaagtacg acgaa
//
LOCUS       DAOTON010000087         4989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_13949_length_4989_cov_2.991690, whole genome shotgun sequence.
ACCESSION   DAOTON010000087 DAOTON010000000
VERSION     DAOTON010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13949_length_4989_cov_2.991690"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="P5292_02715"
     CDS             <1..512
                     /locus_tag="P5292_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRS38068.1"
                     /translation="LFTAIGVFLSSRGPIFYSHERIGLHGKPFRIYKFRSMYTDAEKT
                     GRPQLSSHNDPRMTPFGRWMRKIRLDEIPQFWNVLKGDMSIVGPRPERQFFIDQIMQV
                     APHYRHLQKVRPGITSWGQVKFGYAENVEQMVERLKYDLIYIENMSLALDFKIMTYTV
                     LIILKHKGK"
     gene            764..1654
                     /locus_tag="P5292_02720"
     CDS             764..1654
                     /locus_tag="P5292_02720"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRS38069.1"
                     /translation="MKNISVIGAGTMGNGIAHVFAQSGYQVAMIDVSEDALKRAVDTI
                     SKNLDRMVAKEKISVQDKESTLKHIRTFTSLQEGVAGADLVVEAATENVDLKCRIFRD
                     MDQHAPAGAILATNTSSISITLMAAQTKRPEKVIGMHFMNPVPIMKLVEVIRGYATSD
                     EVTGTILELSRKLGKVPVEVNDYPGFVSNRILMPMINEAIETLFQGVAGVEEIDTVMK
                     LGMAHPMGPLQLADFIGLDTCLSILRVLQNGFGNPKYAPCPLLVNMVTTGNLGVKSGK
                     GFYSYSAGSKELVVAPEFRR"
     gene            complement(1831..2421)
                     /locus_tag="P5292_02725"
     CDS             complement(1831..2421)
                     /locus_tag="P5292_02725"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HRS38070.1"
                     /translation="MKTLLLLCCCLLSLGAVAQKGDPQGPYKLPYILEGDDTIPVVNL
                     PIVDIVESGADYQKNLKEYYRLRYNVIKVYPYARLAAVKVREMNARMEKMDSEREKRR
                     YRKAVEDQIRKDFEEQIKKLSINQGNVLIKLIDRETGQSSYSLIKELKGSFNAFFAQS
                     LARLFGHDLKDRYDPTGADKTIENIVRQIETGQIAF"
     gene            complement(2458..3951)
                     /gene="accC"
                     /locus_tag="P5292_02730"
     CDS             complement(2458..3951)
                     /gene="accC"
                     /locus_tag="P5292_02730"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRS38071.1"
                     /translation="MKKILIANRGEIAIRVMRSAREMGIATVAVFSEADRNALHVRYA
                     DEAVCIGPAASSQSYLVMERIIEAARQTGADAIHPGYGFLSENAAFADLVKASGIIFI
                     GPSGDSMRKMGSKLAAKATAEAHNIPLVPGTAKALESVEEGMRVAEKIGFPVLIKASA
                     GGGGKGMRIVHAAVEFEEQMRLAVSEATSSFGDGAVFIERYVGSPRHIEIQVLGDTHG
                     NVVHLFERECSVQRRHQKVVEEAPSSVLTPQLRAEMGRCAVEVAKACNYVGAGTVEFL
                     LDERLNFYFLEMNTRLQVEHPVTEYITGLDLVKEQIKVARGERLSFRQEDLKILGHAI
                     EIRVYAEDPADNFLPDIGRLSTYRIPQGPGIRVDDAYEEGMDIPIYYDPMIAKLIAYG
                     SDRSEAIERMKRAIEDYHITGVKTTLPFCHFVMRHEAFVSGKFDTHFVKNHYTPDMLH
                     RAEPDEEMIAALLAVRSIRNNSIPRNSTSGEQGKVSRWKSNRLRSAD"
     gene            complement(4020..4673)
                     /locus_tag="P5292_02735"
     CDS             complement(4020..4673)
                     /locus_tag="P5292_02735"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRS38072.1"
                     /translation="MKDFKIDNKGSGRLFSSSFLERLTRTHFLFPVILYYLAAISCVG
                     YAIYETSISFLRFWWLFVPGMLLFSLVEYLIHRFLFHFNAKSPAELQLQYNIHGIHHE
                     FPRDKDRLVMPPVVSIVVASLFGYCFYFLMGGNGWILFGGFLAGYSTYLVIHYSVHAR
                     RPPDNFLRYWWKHHSRHHYASVHSAFAVSMPLWDFVFGTMPPKSDTGRSGATDDQHS"
     gene            complement(4724..>4989)
                     /locus_tag="P5292_02740"
     CDS             complement(4724..>4989)
                     /locus_tag="P5292_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016516808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HRS38073.1"
                     /translation="AIAALPKNVYLTIDADGFDPAVIPAVGTAEPNGLFWVETLQFLR
                     RVFRERNVVGFDVVECAPIEGSILSEYTLAKLVYRLIGYQVTR"
BASE COUNT         1240 a         1306 c         1292 g         1151 t
ORIGIN      
        1 acctgttcac ggccatcggc gtgttccttt catcccgcgg ccccatcttc tacagtcatg
       61 agcgcatcgg cctccacggg aaacccttcc gcatctataa gttccgctcg atgtacaccg
      121 atgcggaaaa gaccggccgg ccgcaactct cgagtcacaa cgatccgcgc atgacgccct
      181 tcggacgctg gatgcgcaag atccgcctgg atgagattcc ccagttttgg aatgtcctca
      241 aaggcgatat gtcgatcgtt ggtccacgcc cggagcggca gttcttcatc gaccagatca
      301 tgcaggtagc accccactac cggcacctcc aaaaagtaag gcccggcatt acctcctggg
      361 gacaggtgaa attcggctac gcggaaaacg tcgaacagat ggtcgaacga ctcaagtacg
      421 acctgatcta tatcgaaaac atgtcgctcg ctcttgactt caagattatg acgtatacgg
      481 tgttgattat tttgaagcat aaggggaagt agtggagagt gaacagtgaa tagtgaacag
      541 tgaacagtga acagtgaaca gtaaacagcg aatagcgaat agcgaatagt gaatagtgaa
      601 tagtgaattt tacctgccga agctttagcg taggcaggag tgaatagtac gttcggagtt
      661 aatacccaat tactaattac tagttactaa ttactagtta ctaattacta gttactaatt
      721 gtaatttcaa gtcaccacca tcatctgatc atccattctt tctatgaaaa acatcagtgt
      781 tatcggggcg gggaccatgg gaaatggtat cgcgcatgtg tttgcccagt cgggttatca
      841 ggtcgcgatg atcgacgtat ctgaagacgc tttgaagcgc gcagtggata cgatttccaa
      901 gaacctggat cggatggtcg ccaaggaaaa gatcagtgtg caggacaagg aaagcaccct
      961 gaagcatatc cggaccttta cgtctttgca ggaaggtgtt gccggtgcag acctcgtggt
     1021 tgaagccgcg acagagaacg tcgacctcaa atgcaggatc ttccgcgaca tggaccagca
     1081 cgctcctgcc ggcgcgatcc tggccaccaa cacctcgtcg atttccatca cgctgatggc
     1141 ggcgcagacg aagcgacccg aaaaagtgat cggcatgcac ttcatgaatc ccgttccgat
     1201 catgaaactg gtggaagtca tacgcggtta cgccacgtcg gatgaagtga ccggaaccat
     1261 tctggaactt tcacgcaaac tcggcaaggt gccggtagaa gtgaacgatt atcccggatt
     1321 cgtttccaac cgcatcctca tgcccatgat caacgaagcc atcgaaaccc ttttccaggg
     1381 cgtagccggc gtagaagaaa tcgacacggt catgaaactc gggatggctc atccgatggg
     1441 tccgctgcaa cttgccgact tcatcgggct ggacacctgc ctgagcatcc tgcgtgtgtt
     1501 acaaaacggc ttcggtaatc cgaaatacgc tccctgtccc ctgctggtca acatggttac
     1561 caccggaaac ctgggcgtca aaagcggaaa gggattttac agctactccg cgggcagtaa
     1621 ggaattggtg gtagcaccgg agttcaggcg gtgaaagtgg aaatagtgat tggggatttg
     1681 aagatggtaa ctagaaacta gtaactggta attagtaatt agtgattagt gattagtagc
     1741 ttgagctgat tattttagaa ttaccaatat ctggttatca gtttctagtt actagtttct
     1801 agttacttgt tacccgcctt cctcccactc tcaaaacgct atctgccccg tctcgatctg
     1861 acgtacgatg ttctcgatcg ttttgtcggc cccggtggga tcgtaacggt cctttaagtc
     1921 gtggccgaat aacctggcca ggctttgtgc aaagaaggcg ttgaaagagc cctttagttc
     1981 ctttatcagg ctataggaag attgtccggt ttcccggtcg atcagtttga tcaggacatt
     2041 gccctggttg atgctgagct ttttgatctg ctcctcgaag tcctttcgaa tttggtcttc
     2101 taccgccttc cggtaccggc gtttttcacg ctcgctgtcc atcttctcca ttcttgcatt
     2161 catctcccta actttcactg ctgcaagacg cgcataggga tagactttga tcacattgta
     2221 tcgaagccgg tagtactctt tgaggttctt ctggtagtcc gcaccgcttt cgacgatatc
     2281 gacgatcgga aggttgacga ccggaatggt atcgtctcct tcgagtatat aggggagctt
     2341 gtaggggccc tgcgggtcac ctttttgtgc gacagcgcca agggaaagca gacaacagca
     2401 caacagcaaa agcgttttca tcccgtcctt gattacaaag cacgtgccga attaaagtca
     2461 gtcagcagac cttaacctgt ttgatttcca gcgtgaaacc ttcccctgct ctccggaagt
     2521 ggaatttcgg ggtatggaat tattccgaat actccggaca gccagcaagg ccgcgatcat
     2581 ctcctcatcg ggctccgcac ggtgcaacat atcaggagta tagtggttct tgacgaaatg
     2641 ggtatcgaac tttcccgaaa cgaatgcttc atgccgcatc acgaaatggc agaacggtag
     2701 tgtggtcttt accccggtga tgtggtagtc ctcgatcgcc cgcttcatgc gttcgatagc
     2761 ttccgaacga tcgcttccgt aggcgatcag tttggcgatc atcggatcgt aatagatcgg
     2821 aatatccatg ccctcctcat aggcatcgtc cacgcgtatg ccaggtccct gcgggatccg
     2881 gtaggtcgag agtcgaccga tgtccggcag gaaattatcc gccgggtctt ccgcatacac
     2941 ccggatctcg atggcgtgac cgagaatctt caggtcttcc tgacggaatg aaagacgttc
     3001 gcctcgggcg acctttattt gctctttcac taggtcgagg ccggtgatat actctgttac
     3061 cgggtgttct acctggagac gcgtattcat ttccaaaaag tagaagttaa ggcgttcgtc
     3121 gagtagaaat tccacggtgc ctgctcctac gtagttacaa gccttcgcga cttccaccgc
     3181 gcaacgaccc atttccgccc gtagttgagg tgtcaacacg gatgatggcg cttcctctac
     3241 gaccttctga tgcctgcgct ggacactgca ctcccgttcg aacaggtgaa ccacattgcc
     3301 gtgcgtatcg ccgagtacct ggatctcaat gtgacgaggg gaaccgacgt atcgttcgat
     3361 gaatacagct ccgtcaccga aggaggaagt cgcttcgctg accgccaggc gcatttgctc
     3421 ttcgaactca acggctgcat gtacgatgcg catgcctttt cctccaccgc ccgcggatgc
     3481 cttaatcagc accggaaacc cgatcttctc ggctacccgc ataccctctt ctaccgattc
     3541 aagtgccttg gccgtgccgg gtaccagggg aatattatgc gcttcggcgg tcgccttcgc
     3601 ggccagtttg ctgcccatct tacgcatgga atcgccgctc ggtccgatga agatgatacc
     3661 ggaagctttc accaggtccg cgaacgccgc gttctccgac aggaacccat aacccggatg
     3721 gatggcatcg gcgccggttt gacgggcagc ctcgatgatg cgctccatga ccagataact
     3781 ctggctggaa gcagccggtc caatgcaaac tgcttcgtcg gcatagcgca cgtgcagggc
     3841 attccgatcg gcttcagaaa acaccgcaac ggtagcgata cccatttcac gggcagagcg
     3901 catgacccgg atcgcgatct cgccccggtt ggcgatcaat atcttcttca taacaggtta
     3961 ttggccttcg aaaggtacgg aatccgttga aaggggattc cctcaataca atgaagtaat
     4021 caggaatgct gatcatcggt cgcaccggac cttcccgtat ccgatttcgg tggcatcgta
     4081 ccgaagacga agtcccacaa gggcatcgag accgcaaagg ccgaatgcac cgaggcatag
     4141 tggtgacggc tgtgatgctt ccaccagtac cgcaggaaat tgtcgggagg acgccgggca
     4201 tgcacggaat aatggatcac caggtaagtg gaataaccgg ccagaaatcc tccgaagagt
     4261 atccacccgt ttcctcccat caggaagtaa aagcagtagc cgaagagcga tgctacaact
     4321 atagaaacaa ccggcggcat gacgagtcgg tccttgtcgc gcgggaattc atgatggatc
     4381 ccgtgaatgt tatactggag ttgaagttca gccggcgact ttgcgttaaa atgaaaaagg
     4441 aagcgatgga tgaggtactc gaccagggag aagagcaaca tgccgggaac aaacaaccac
     4501 caaaatcgaa gaaacgagat ggatgtttcg tagatcgcat aacccacaca ggagatcgcg
     4561 gccagatagt acaagatgac cggaaacaaa aagtgcgttc tggtgagtcg ctcgaggaat
     4621 gaactggaaa aaaggcgacc cgatcctttg ttgtcaatct tgaaatcctt catgattgta
     4681 acgctgggtg cattacaaaa ttaaggatta aaaaaccgaa atatcaacgg gtaacctggt
     4741 agccgatcag gcggtacacg agcttcgcca gggtatactc agacaggata ctgccttcga
     4801 tgggagcaca ttcgacgaca tcgaaaccga caacattccg ttcacggaag acacggcgaa
     4861 gaaactgcag cgtttccacc cagaaaagac cattgggctc agccgtaccg actgccggaa
     4921 taacagcagg atcgaaacca tccgcatcga tcgtcaggta cacgttcttc ggaagggcag
     4981 cgatggcct
//
LOCUS       DAOTON010000088         4937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_14117_length_4937_cov_3.602827, whole genome shotgun sequence.
ACCESSION   DAOTON010000088 DAOTON010000000
VERSION     DAOTON010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14117_length_4937_cov_3.602827"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2048
                     /locus_tag="P5292_02745"
     CDS             <1..2048
                     /locus_tag="P5292_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRS38074.1"
                     /translation="HPDYHEKARSLFEQLNKTGKLSDISVPLVNNDGSTYWVRTNATS
                     EKAANGQLLHKVNTVEITEGRHNLEKVRFQSKLLEECNDAIISVDLETRVTSWNKGAE
                     RMYGYQESEALGIKVNDLMGLDKPFSATELEAMITALLSNKRLIGERTHHNKAGEKIN
                     VLYSISPLMNERGEIIGAVSVFQDISLRKRFEEQLMDFNISLEQKVRENTEEFKQLVT
                     GISDAFIALDHQLRVKFLNMKAAELLEGQPGDFLGHYLTKSTSIPNASVWIEFLQRVL
                     LSKQPETFELKCSESVTSYMCRAYPTESGITLLIENSTERLRAQQLLQESEEKYRLFF
                     ENSPLPLYIFELTNLQVVDANMAACRQYGYDKEQLIGISALDMVQSHDQQSVREHLQA
                     ISEGKRFEGTCMHQRKDRTPMQVEMFSETIHRNGKEYRLVLAYDISARVAAEEELKRS
                     RDEFRKLNQHLENIREEERTKIARELHDEIGQQMTVMKMDLALLLRKATRDESRVTLN
                     GMIALADTTIATIRRISSSLRPGILDDLGLLSAIQWHCKEMSNHAKIPIRVISDGTDY
                     DFDPETNTAIFRIVQESLTNILRHAKASYIEIDVHTADDRWMITVRDNGVGFEEDPAL
                     YGLGLLGMRERARALDAKLELTSRPGFGTELRLSLPLACPMGTVDQPTHQLDAYTAD"
     gene            2029..2655
                     /locus_tag="P5292_02750"
     CDS             2029..2655
                     /locus_tag="P5292_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS38075.1"
                     /translation="MRILLIDDHAIVRKGLRQILTEEWPDARYGEAGSFHDGQLAATG
                     EEWDVIISDVSLPDKSGIELVKAIVSQGIQTPILILSIHPEELYASKALRAGACGYLP
                     KETAPNDLIVAVKTILAGNRFLTESMRRSLSASIEREKEQPAHELLSEREFQVMILLS
                     SGKNLTTIADNLNLSINTVSTYRTRILEKLKVDSTAALMHYAISHRLV"
     gene            2814..3191
                     /locus_tag="P5292_02755"
     CDS             2814..3191
                     /locus_tag="P5292_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS38076.1"
                     /translation="MKLKVLIADDSDDIVRRLRNMVLDLDYVSGIGHARNGDEALVVT
                     NFMQPSVVILDIRMPGRSGIETLKEIKQNLPQTTVIILTNHSEEEWRSHCLDAGADHF
                     LDKSKEFDKIPSILGAIAGNPVN"
     gene            3249..>4937
                     /locus_tag="P5292_02760"
     CDS             3249..>4937
                     /locus_tag="P5292_02760"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRS38077.1"
                     /translation="MKKPGFKYLLMVIVFLLTIGYFVTSSIRHSRLTDETAQRVKLVL
                     DRMIVYENIYDDMQDFETGYRGYVITRDSDYLEPIDRHTREIPLDITKLRSLTNQEGI
                     SAAEASDIHNLDSLVQKKIEHGKVVIQLVHDGKQDEAFQRVSSGYGKDLMEKIRTVIS
                     SLESSGRYQLQVANTRSSEAAARTRNDYLLSGVIGFSLIGLLLYFLYLEEKRRIRLGR
                     EAAHFAAMVDQSQEAIFSVGTDSRILSWNKGAEMMYRIPEQDAVNKRFLDVVRPVMSQ
                     EELSSISQHLKTSSHWEGEQIHLRQDGVPIRVISSTNVLLDDKGIKVGYVAHTKDITR
                     IKELEEELQQINSNLAGQVAEKTREIREIFERVTDGFLAVDSKWNITFISDNGAVLLK
                     LEKASALQRNFWDLIPMKYSGEFVQGCEEALLRQRPVELEAYYDPAGSWFQYRIHPSA
                     NGLSVFFRDVTVEKRVMEELHRSEQRFRVLVENSRNLIAQFNEHQQLTYINPAGAFIL
                     GIPSPEKPQIPFIIQQQLDAVIRKVLQDGAPNEQLVSWKKEEHSCHLLVRVAMVQ"
BASE COUNT         1327 a         1290 c         1235 g         1085 t
ORIGIN      
        1 ttcatcccga ctaccacgag aaagcccgat ccttgttcga acaattgaac aaaaccggca
       61 agttgagcga tatcagtgtc ccactcgtga acaacgatgg atccacatac tgggttcgta
      121 ccaacgcgac atcggaaaag gccgcgaacg gtcaattgct gcacaaagtc aatacggtcg
      181 agataacgga aggccgccac aacctggaaa aggtccgctt ccagtccaag ctactggagg
      241 aatgcaatga tgcgatcata tcggtagatc tcgagacccg ggtgaccagt tggaacaaag
      301 gggcggaacg catgtatggt tatcaggaat cggaagcgct gggaataaag gtcaacgatc
      361 tcatggggct cgacaaacca ttttctgcga ctgagctgga ggcgatgatc acagccctac
      421 tttcgaacaa acgtttgata ggagaacgaa cccatcataa taaagccggg gaaaagatca
      481 atgtacttta ttcaatttct cccttgatga acgagagggg cgaaataatc ggtgccgttt
      541 cggtttttca ggacatctcg cttcgtaagc gcttcgaaga gcaacttatg gatttcaaca
      601 tttccctgga acagaaagtc agggaaaaca cggaagagtt caaacagctt gttaccggaa
      661 tctctgatgc atttatcgcc ttggaccacc agttacgtgt caaattcctc aacatgaaag
      721 ctgccgagtt gcttgaaggg caacctgggg attttctcgg acattacctg accaagtcca
      781 catcgatacc gaatgcgtcc gtctggatag aattccttca acgagtactg ctatcaaaac
      841 aaccggagac cttcgaatta aaatgctctg aatccgtcac aagttacatg tgtcgggctt
      901 acccgaccga atccgggata acactgctga ttgaaaacag tacggaacga cttcgtgcac
      961 aacaattact gcaggaatcg gaagaaaagt accgcttgtt cttcgaaaac agcccgctgc
     1021 cactctacat tttcgaactg accaaccttc aggtggtcga cgcgaacatg gctgcatgcc
     1081 gacaatatgg ctatgacaag gagcaattga tcggcatctc cgcactcgac atggtgcaga
     1141 gccatgatca gcaatccgtt cgcgaacatt tacaagccat cagcgagggt aagcgattcg
     1201 aaggcacttg catgcaccaa cgcaaggacc gcacgcccat gcaggtggag atgttctccg
     1261 aaaccattca ccgaaatggt aaggaataca gactggtact ggcctacgac atcagtgccc
     1321 gtgttgccgc tgaagaagaa ctcaagcgtt cccgcgacga attccggaaa ctcaaccagc
     1381 atcttgaaaa tatccgggag gaagaacgta caaagatcgc ccgcgaattg cacgatgaga
     1441 tcggacaaca gatgaccgtc atgaaaatgg accttgcgct cctcctccga aaagccaccc
     1501 gggatgaatc aagggtcacc ttgaacggca tgatcgcgct tgccgacacg accattgcca
     1561 ccattcgacg gatcagctcc agcttacggc ccggcatatt ggatgacctt ggcttgcttt
     1621 ccgccatcca atggcattgc aaggagatga gcaatcacgc caagattccc attcgcgtga
     1681 tcagcgacgg tacggattac gatttcgacc ccgaaaccaa tacggcgatc tttcgcattg
     1741 tacaagagag cctcaccaac atcctgcgtc acgccaaagc cagctacatt gaaatcgatg
     1801 ttcatactgc cgacgatcgc tggatgatta cggttcggga caacggggtc ggttttgaag
     1861 aagatcctgc cctctatggt cttggattac ttggcatgcg tgaacgcgca cgtgcgctgg
     1921 acgcgaaact ggagcttact tcccgacccg ggttcggaac ggaacttcgc ctgagtttgc
     1981 ctttagcctg cccgatggga acggtcgacc aacctactca ccaactcgat gcgtatactg
     2041 ctgattgacg accacgcgat tgtccgcaaa ggcctccggc aaatcctgac cgaagaatgg
     2101 ccggacgccc gctacggcga agccggaagt tttcatgatg gtcaactagc cgccactggt
     2161 gaagaatggg acgtcatcat cagcgacgtc agtctgcccg acaaatccgg tattgagttg
     2221 gtcaaagcca ttgtatcgca aggcatacaa actccgatcc tgatcctgtc cattcacccg
     2281 gaagaattgt atgccagcaa ggcgcttcgt gccggcgcct gtggatatct gccgaaggaa
     2341 acggccccca atgacctgat cgtagcggtc aagaccatcc tggccggaaa ccgctttctg
     2401 acggaaagta tgcggcgttc cctgagcgct tccatcgagc gggaaaaaga acaaccggcc
     2461 cacgaactat tgtccgaacg ggaattccag gtgatgatcc tgttgtcaag cggtaaaaac
     2521 cttacaacaa tcgccgacaa cctgaatttg agtatcaata cggtcagcac ctaccggacc
     2581 cggattcttg aaaagttgaa agtggatagt accgctgcgc tcatgcacta cgcgatcagt
     2641 caccggctcg tctaaacaat caacattttt ctggtggtat acccccaaaa gagggcacct
     2701 gcttgtagta caaaaaacca caagcattca tccgtttttc tgattcttac ataggatgga
     2761 acctgacatg ctgtttttcg tttctttaag agggaattcg ctttcccggt ttgatgaaat
     2821 tgaaagttct catagctgat gattccgacg acattgtcag acgcctgcgc aacatggtgc
     2881 tggacttgga ttatgtcagc ggtatcggtc atgcgcgcaa cggtgatgag gcgctggtcg
     2941 ttacgaattt catgcagccg tccgttgtga tattggatat ccgaatgccc ggacgctcgg
     3001 ggattgaaac gctcaaagag atcaaacaaa accttccgca gaccaccgtg atcatcctga
     3061 ccaatcattc cgaagaagaa tggaggtcac attgcctgga tgccggtgct gatcattttt
     3121 tagacaaatc gaaggagttt gacaagatac cttcgatcct gggagccatc gccgggaatc
     3181 cagtgaactg attccgtatt gggatcacct tttctgagta atttcctact tttagattta
     3241 ctttcaatat gaagaagccg ggattcaaat acctgctgat ggttattgtc tttttactca
     3301 ccattggcta cttcgtaacc tccagtatcc gccactcgcg cttaaccgat gaaacggcac
     3361 aacgggtaaa gttggtcctg gatcgcatga tcgtttatga gaacatttac gatgatatgc
     3421 aggatttcga aacaggttat cggggatacg taatcacacg cgattccgat tacctggaac
     3481 cgattgaccg gcatacaagg gagatcccgc ttgacatcac caaattacgc agcctgacaa
     3541 accaggaagg gatcagcgct gctgaagctt ccgacatcca taacctggac tccctggtac
     3601 agaagaaaat agaacatgga aaggttgtca ttcaactggt tcatgacggg aaacaggacg
     3661 aggcgtttca gcgtgtcagc tccggctatg gcaaggacct gatggaaaaa atccggacgg
     3721 taatttcatc actcgaaagt tccgggcgat atcagttgca ggtagccaat acgcggagct
     3781 cggaggccgc ggcaagaaca aggaacgatt acctcttatc cggcgtcatc ggcttttcat
     3841 tgatcgggct tttgctctac tttctctacc tcgaagaaaa gcgaagaatc cgactggggc
     3901 gggaagctgc tcacttcgcc gcgatggtgg accagtcgca ggaagcgatt ttctccgtag
     3961 gaacggatag ccgcattctc agttggaaca aaggcgcgga aatgatgtac aggattcctg
     4021 aacaagatgc ggtcaacaaa cggttcctgg atgtggttcg cccggtcatg agccaggaag
     4081 aattatcttc catcagtcaa cacctcaaaa catccagcca ctgggaaggc gaacagattc
     4141 atctccgaca ggatggcgtt cccatccggg tcatttcctc caccaacgtg ttgttggacg
     4201 ataaagggat caaagtcggg tatgtcgccc atacgaagga cattacccgc atcaaggaac
     4261 tcgaagaaga actccagcag atcaacagca acctggccgg ccaggtcgca gaaaagaccc
     4321 gggaaatacg ggagatcttc gagcgggtga cggacggttt tctggcggtc gattcgaagt
     4381 ggaacatcac ctttatcagc gacaatggcg ctgttctgct gaaactggaa aaagcttccg
     4441 cgcttcagcg gaatttctgg gacctaattc cgatgaaata ttccggtgaa ttcgtccagg
     4501 gctgcgagga agccctgctc aggcagcgcc cggtcgaact ggaagcctac tatgatcctg
     4561 ccggatcctg gtttcagtac cgaatccatc cgtctgccaa tggcttgtcg gtcttcttcc
     4621 gggatgtcac agtcgaaaaa cgggtaatgg aagaactcca ccgaagcgaa cagcgctttc
     4681 gtgtattggt cgaaaattcc cgtaacctca tcgcccaatt caacgagcac cagcaactca
     4741 cgtatatcaa ccccgccggc gcgttcatcc tgggtatccc ctctcccgaa aagccgcaga
     4801 tcccgttcat catccaacag caactcgatg cggtcatccg gaaagtgctg caggatggcg
     4861 cccccaacga acagttggtg agctggaaaa aggaagagca ctcctgccac ctgctggtgc
     4921 gggtagcgat ggtccaa
//
LOCUS       DAOTON010000089         4932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_14130_length_4932_cov_3.040804, whole genome shotgun sequence.
ACCESSION   DAOTON010000089 DAOTON010000000
VERSION     DAOTON010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14130_length_4932_cov_3.040804"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="P5292_02765"
     CDS             complement(<1..1353)
                     /locus_tag="P5292_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRS38078.1"
                     /translation="MNRQSRAEEGRRVLYRLLLIVVAVVYGLVFVLDYLLQLHFTFFL
                     LLSLFGFVVLVLFYMGFRVSTTVESREPVTEPDDRKGPELNEDDTTTEAGSLIDAGLS
                     EWPEPMIRINPVTGLMVDVSPGALRLLGAKGFDDVIGVDLYTRFRDTWTAEERQQFRL
                     RLAAENRAAAAVELSGFDGVTRKVLLQVSRSEMAGQRMLLVHLTDLSLLPEASGIRPQ
                     PPADAYQAVFSEGLLPMAFFGIDYRIQRANKAFCDLLGYQEEELRGMTAIELVHPEER
                     EQERQILSRLLRGELPVARREKRFIRRNHDVIWVQTSASVSQGIGGTPAFVITMAENI
                     TQRKRIERSLSDSRQRLNALVENAEYAILSVDVRHTILLINSRLAALLFALTGIVVET
                     GYNVLDILPDQFRDDFLSLHARAMAGESLTIEKQLVLTGKRIDVEIIATPVRNEHRLV
                     V"
     gene            complement(1662..3389)
                     /locus_tag="P5292_02770"
     CDS             complement(1662..3389)
                     /locus_tag="P5292_02770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRS38079.1"
                     /translation="METASTYFTAPVKAPRRFLPDTLEIDSWDALSPYFKELEERLLH
                     SADGLKQWLEDLSELESVVQEHVGWLYIRMTCNTQDEALVKAYTFFINEINPHIEPFA
                     DRLNRKLLDCPFRNGLDKRFSIHLRSTENEIRLFREENIPLNAELSTREQKYGEIAGA
                     MSVELNGKTLTLQQAANFLREPNRGVRQSAYEKIVSRRLQDREKLDSLFDELLMLRHQ
                     IANNSGFSNYRDYKFVAMDRFDYTADDCMRFHDSVRQAIVPVLDKLMEERQRDMGLES
                     LKPWDLDVDSKGRPDLKPFSDGSDLMRKTIACFSEIDPYFGFVARTLDEMRFIDLDSR
                     IGKAPGGYNYPLYETGVPFIFMNASGSFRDVVTMVHEGGHAIHSMLTRDLEFVGFKEL
                     TSEIAELASMSMELISMEHWHHFFPNEEELRRAKREQLENVLEALPWIACVDRFQHWL
                     YTHPGHTVEERTAAWDEVYRAFSPSRVDWSGHEPVRKSLWQKQLHLFEVPFYYIEYGM
                     AQLGAIALWRNYRNDPKTAIEQYKQALSLGYTRSIPEVYQAAGIRFDFSENYIQELAA
                     FVQEELEKL"
     gene            complement(3389..3904)
                     /locus_tag="P5292_02775"
     CDS             complement(3389..3904)
                     /locus_tag="P5292_02775"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HRS38080.1"
                     /translation="MEFKDILAVPGMPGLYKVVGNNKNGFIVESILDGKRSMVNGQQR
                     IMTLIDIAVYTSGDEKPLREIFLSLQEKTNGKLPVDLKGDDKKIRDYFRTIVPDFDEE
                     RVYNSDIKKMLNWFQLLNGKVDFTKPVEADAAAVKKEDDKPVVPKVHEAHGPKAEQHA
                     KTSTARTRKKV"
     gene            complement(4005..>4932)
                     /locus_tag="P5292_02780"
     CDS             complement(4005..>4932)
                     /locus_tag="P5292_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38081.1"
                     /translation="VSGQEPGTVSDFDAYGQFVDNGTTSSTPLPVLVSHRAYAWAAPA
                     ADRKYIIVDYTIRNNGVNTLNNLYAGLFADWDIPQYGNNKSDTDAGRKMGYTWSTDAG
                     GLWAGMKLLTSGGFNHYAVDNVTGGNGGLDLSDGFSNTEKYSALSSSRSPAGNTASTG
                     NDVIDVVSSGPFALAAGDSIRVAFALIAGEDLTMIQASADAAQVRWDNTVLTTGLIAL
                     PSSETFGLSTIYPNPAGSNAMLRFTLDRSGKVELSIYNALGERVQSVLAQTLGVGSYS
                     TGVDLNKLESGTYFVRLTCDGQSATRPLQVVH"
BASE COUNT         1174 a         1386 c         1280 g         1092 t
ORIGIN      
        1 gaccactaac cggtgctcat tcctaaccgg cgtcgcgatg atctccacat cgattctttt
       61 gcctgtcagc accagttgct tctcgatggt aagcgattca ccggccattg cccgcgcatg
      121 caaggacagg aaatcgtcgc ggaattgatc cggaagaata tccaggacgt tatatccggt
      181 ctctactacg atgccggtaa gggcgaagag cagagcggcc aagcgtgagt tgatcagcag
      241 gatcgtatgc cgtacgtcga ccgagaggat cgcgtactcg gcattctcga ccaaggcgtt
      301 cagccgctgc cgactgtccg acaaggatcg ttcgatccgt ttccgttggg tgatgttttc
      361 cgccatcgta atgacgaagg ccggcgtgcc gccaataccc tggctgaccg aggcggatgt
      421 ttgtacccag atcacatcgt ggttccgacg gataaagcgc ttctcccgcc gggcaaccgg
      481 cagctcaccc cgcagcagac gtgataatat ttgccgctcc tgctcacgtt cttcagggtg
      541 gaccagttcg atggcggtca taccgcgcag ttcttcttcc tggtagccca acaggtcgca
      601 gaaagctttg ttcgcgcgtt gtatccggta gtcgatgccg aagaatgcca taggaagcaa
      661 tccttcggag aatacggcct gataggcatc ggcaggaggt tgcggacgaa tgccactggc
      721 ttccggcaac aaggaaaggt ccgtcaaatg aaccagcagc atgcgctgtc cggccatttc
      781 ggaacgactg acctgcagga gtactttacg cgttacgccg tcgaatcccg acagttccac
      841 cgcggctgcg gcacgatttt ccgcggccaa acgaaggcgg aattgctggc gttcttctgc
      901 agtccaggta tcccggaatc gcgtatataa atcgactccg atcacatcat caaacccttt
      961 agcgcccagc aaccgcaaag ctccgggtga aacgtctacc atcaaaccgg taaccggatt
     1021 gatacgaatc atcggctcag gccattcgct gaggccggcg tcgatcaatg aacccgcctc
     1081 tgtggtcgta tcatcctcat tcagctccgg ccccttgcga tcatccggct ctgtaactgg
     1141 ctcgcgcgat tcaaccgtag tcgaaacgcg aaaccccatg taaaacagca caaggacaac
     1201 gaaaccgaag aggctcagca gcaagaagaa cgtaaaatgc aattgcagga gatagtccaa
     1261 tacgaacacg agcccgtaca ccacagccac tacgatgagc agcaagcggt acagcacacg
     1321 gcggccttct tcagcacgcg attgtcggtt cattggacgc atcgatccca agcagggagt
     1381 tcctgaaaat cagtgtatcc ttatatggat tcggaagccc caggttgtag agaaggagcg
     1441 tcagttaccg gtaatatgcc cgcgggcatt acacgggtaa cgcgtttccg ttttcaagtc
     1501 gaatcttaca atttggaacc gtgtcatcaa aagggaaatg cgccccgcac cagaagcgcc
     1561 attaacacgg gaaattcgcg gcaattccga caaagcagtc caggctgaac atgcgcgagc
     1621 ggctcatcag ctccctgaaa caaaaaaacc caggcttttc cttacagttt ttccagctcc
     1681 tcttggacaa acgcagccag ctcctggatg tagttttccg agaaatcaaa ccggataccg
     1741 gccgcctgat agacctccgg aatcgagcgg gtatagccca gggaaagcgc ctgtttgtac
     1801 tgttcaatag ccgttttcgg gtcgttccgg taattcctcc aaagggcaat ggcacccagt
     1861 tgcgccattc cgtactcgat gtaatagaaa ggcacttcaa agagatggag ctgtttctgc
     1921 cacaaggatt tcctaaccgg ctcatgcccg ctccagtcaa ccctggaagg actgaaagcg
     1981 cgataaacct catcccaggc agcagtacgt tcttcaaccg tatgaccagg gtgggtatac
     2041 aaccaatgct ggaaccgatc aacacacgcg atccagggca atgcttccag cacattttcc
     2101 aactgctcac gtttggcgcg acgcaactcc tcctcgttcg ggaaaaagtg atgccagtgt
     2161 tccatggaga tcaactccat gctcatggac gcgagttccg cgatctccga cgtcagctcc
     2221 ttgaacccta cgaattcgag atcgcgcgta agcatcgagt ggatcgcatg tccaccctcg
     2281 tgcaccatgg tgaccacatc gcggaaagag ccacttgcat tcatgaagat gaacggtact
     2341 ccggtctcgt acaggggata attatatccg ccaggcgctt tgccgatccg ggaatccaga
     2401 tcgatgaacc gcatttcatc caacgttcgg gcaacgaaac cgaaataggg atcgatctcc
     2461 gagaagcagg caatcgtctt tcgcatcaga tcgcttccat ccgaaaatgg tttaagatcc
     2521 ggccgccctt tggaatcaac gtcaaggtcc cagggcttca gcgactcgag tcccatgtcg
     2581 cgctgacgtt cttccattaa cttgtccagt accggaacaa tcgcctgccg caccgaatca
     2641 tggaaacgca tacaatcatc agcggtgtaa tcgaaccgat ccatggctac gaacttgtaa
     2701 tcacggtaat tcgaaaaacc ggaattgttg gcgatctgat ggcgcagcat caggagttca
     2761 tcaaacaggc tatcgagctt ctcccggtcc tgcaagcgac ggctgacgat cttctcgtag
     2821 gccgattgac gcacgccacg attcggctcc cgaaggaaat tggccgcctg ttggagcgtc
     2881 agggtcttgc cgttgagctc aacgctcatg gcgccggcga tctcaccgta cttctgctca
     2941 cgggtggaaa gctcggcatt caggggaatg ttctcttccc ggaatagtcg gatctcattc
     3001 tccgtcgacc gcaggtgaat cgaaaatcgt ttgtccagac cattccggaa cggacaatcc
     3061 agcaatttcc tgttcaagcg atccgcgaac ggctcgatgt gcgggttgat ctcattgatg
     3121 aaaaatgtat acgccttcac cagggcttcg tcctgcgtgt tacaggtcat ccggatgtac
     3181 aaccaaccta cgtgctcctg aacaaccgat tccaattcac tcaggtcttc cagccattgc
     3241 ttgagaccgt ctgccgaatg gagaagtcgt tcctccagct ccttgaaata gggcgaaagg
     3301 gcgtcccagg aatcgatttc cagcgtatcc ggcagaaatc ggcgcggcgc ttttaccgga
     3361 gctgtgaagt aggtggaggc tgtttccatt ataccttttt ccgggttcgg gctgtggacg
     3421 tcttggcgtg ctgctccgct ttgggaccat gtgcttcgtg cactttcggt accacaggtt
     3481 tgtcgtcttc tttcttgacg gcagcggcat cagcttctac aggcttggtg aaatccacct
     3541 taccattcag caactgaaac cagttgagca tcttcttgat gtccgaattg tacacccgct
     3601 cttcatcaaa gtccggaaca atagtccgga agtaatcccg gatcttcttg tcgtcgccct
     3661 tcaaatcgac cggcaatttt ccattggttt tctcctgaag actcaggaag atctcacgca
     3721 acggcttctc atcaccgctg gtataaacgg cgatatcgat cagggtcatg atgcgttgct
     3781 ggccattgac catggaacgc ttaccatcga ggatggactc cacgataaaa ccgttcttgt
     3841 tgttaccgac gactttgtaa agtcccggca ttcccggaac ggcaagaatg tctttgaatt
     3901 ccataataag attgaattag aacaaaggta aagtttttag gccttcccaa aacgcgaacg
     3961 ggccggtgaa accaccggcc cgtcgaagta aaagttcgct cccgtcaatg taccacctgc
     4021 agcgggcgcg tcgcggactg accgtcgcag gtcaggcgca cgaaataggt tcctgactcg
     4081 agtttgttca ggtcaacacc cgtggaataa gaacccactc cgagggtctg tgcaagaact
     4141 gattgtacgc gttcacccag tgcattgtag atggaaagtt ccactttgcc ggaacggtcc
     4201 agcgtgaagc gcaacatggc attgctgcct gccgggttcg gatagatcgt cgacaaacca
     4261 aacgtttcgg atgacggaag tgcgatcagt cccgtggtca ggactgtatt gtcccaacgc
     4321 acttgcgctg catccgccga ggcctggatc atggtcagat cttcgccggc gatgagggcg
     4381 aatgcgaccc ggatggaatc tccggcggca agcgcaaagg gaccgctcga cactacgtcg
     4441 atcacgtcgt tacccgttga cgcggtattt cctgcagggc tgcgggaaga agagagtgcg
     4501 gaatatttct ccgtgttgct gaagccatcg gaaagatcca agccgccatt accgccggtc
     4561 acgttgtcga ccgcgtaatg attgaaccct ccggaggtaa ggagtttcat accggcccac
     4621 agtccaccgg catcggtact ccaggtataa cccatcttcc gaccggcatc ggtatccgat
     4681 ttgttgttgc cgtattgcgg aatgtcccaa tcggcgaaca ggcctgcata caggttattc
     4741 agcgtattga ctccattgtt gcgaatggta tagtcgacga tgatgtattt acgatcagca
     4801 gcaggagccg cccaggcata tgcacggtga gaaaccagga ccggtaaggg agtcgaggaa
     4861 gtcgtgccat tgtcaacgaa ctgaccatac gcgtcaaagt cggaaaccgt gccaggctcc
     4921 tggccgctga cg
//
LOCUS       DAOTON010000090         4930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_14140_length_4930_cov_4.043077, whole genome shotgun sequence.
ACCESSION   DAOTON010000090 DAOTON010000000
VERSION     DAOTON010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14140_length_4930_cov_4.043077"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="P5292_02785"
     CDS             complement(<1..469)
                     /locus_tag="P5292_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRS38082.1"
                     /translation="METTGASVVITNHVLSDRITEYEAWLNEIGPICRRSEGLIDWQI
                     IRPVKDLTFTYTVIIRYDSEANLRKWIESDERKRLIEKARPFLRKGDHYVIHSGLDFL
                     FSPEDDSTTSPVRWKQVLITWSAIFPLVLLIPLLILPALRACGIPPSHYFDNLL"
     gene            complement(494..844)
                     /locus_tag="P5292_02790"
     CDS             complement(494..844)
                     /locus_tag="P5292_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRS38083.1"
                     /translation="MLRSVHPKLPMRQVAVTRSFYTEKLGFTVTADYGNYLIVEREKV
                     ELHFFEFVSLDPKENYGQVYIRVSDIDQLHRSFREAGVPLLPKEQPENKPWGQREFSL
                     LDPDHNLLTFGQEV"
     gene            complement(921..1703)
                     /locus_tag="P5292_02795"
     CDS             complement(921..1703)
                     /locus_tag="P5292_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRS38084.1"
                     /translation="MGSQAAQNQLWSRGADDWAAIQEGTVEDCYRYVLEYLPALKDKR
                     VLDIGCGSGLFCHLAQTHSAIVTGMDASDGLIAAARKRNARVDFSVGEMERLPFDDEA
                     FDLVCGFNSFQFAANPLNAFAEARRVLKPGGTLVAMIWGEREDCEAATFMKALGGLMP
                     STMPPGAGPFALTSDRKLENLIGQAGFTIHHQVDRENAWIYPDQDTALRGLLSAGPAA
                     AAIAHSGEERVRTELRQSMQPFVKPDGRVIYRNKFRIVIAKK"
     gene            complement(1719..2216)
                     /locus_tag="P5292_02800"
     CDS             complement(1719..2216)
                     /locus_tag="P5292_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38085.1"
                     /translation="MKQMRILHRYLGYFLTGMMAVYAVTGFIMTFRDTNFLKFDKTWE
                     RTVEPNLPGSVLGEAIEQRRLKVTREDSTTIYFDNGQYDKASGKATFTTKEWPAYIEK
                     LTDLHTSRTADPLFFMNVFFACSLFFFVVSSFWMFPPQAKTFKKGMWFVGAGVILVVI
                     MLISR"
     gene            complement(2411..2749)
                     /locus_tag="P5292_02805"
     CDS             complement(2411..2749)
                     /locus_tag="P5292_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRS38086.1"
                     /translation="MEEEINAWLADILNAIGEIEAFLPVEKNFLTFQEDLRTRRAIER
                     NIEIMGESMSRILRVRPEISISSARKIVDTRNRISHGYDSVSAEILWSIVIRDLPLLK
                     REVQLLLPPS"
     gene            complement(2733..3071)
                     /locus_tag="P5292_02810"
     CDS             complement(2733..3071)
                     /locus_tag="P5292_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRS38087.1"
                     /translation="MLIVHYLAGAMDLISQNISQIRALCDQYHVRYLFAFGSAVRNDL
                     KAGSDVDFLVDFESSDPIIYSDNYFALKFNLQQLFDRPIDLLEMKALSNQFIKQRIDQ
                     TKVLVYGRGN"
     gene            complement(3183..3632)
                     /locus_tag="P5292_02815"
     CDS             complement(3183..3632)
                     /locus_tag="P5292_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38088.1"
                     /translation="MMKHTRFLLFAIAFSLFACNSSTIKEKINQAGDVAGQTAGEFIE
                     GASKGVQKAFDVKVELSESLKSKGIELGKSTVSSDSLGTDNLLSQYVIFNQDFSGALT
                     ARVFDSSDKEMGRAAVEVEGKQKEARFIDFRFDPRTNIDSKCRLNVE"
     gene            complement(3734..4099)
                     /locus_tag="P5292_02820"
     CDS             complement(3734..4099)
                     /locus_tag="P5292_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HRS38089.1"
                     /translation="MQQNEKQRQRIAMMTFASVYPLYLQKVTRKGRTEAELREVINWL
                     TGYDGKRLDAVIHDKLTFEAFFQQARLHPNAHLITGLICGYRIEEIEDPLTRQVRYLD
                     KLVDELAKGRTMEKILRKA"
     gene            complement(4223..4699)
                     /locus_tag="P5292_02825"
     CDS             complement(4223..4699)
                     /locus_tag="P5292_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38090.1"
                     /translation="MKRTSIRLLLLRIPLRLLLWSLVIIPLMVEGRMPVDSLLTEKQV
                     YTSREPIGFTVRLSSEAKPGTVRVIPTMSCSCSDRDFYYVVYRLGKGRGSRSRTVFLN
                     HSEAQTAPRCQCKVRYANFKEGGTWSIPAINKRGRYQIEVRSSSSILNSNVFVVRE"
     gene            complement(4716..>4930)
                     /locus_tag="P5292_02830"
     CDS             complement(4716..>4930)
                     /locus_tag="P5292_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRS38091.1"
                     /translation="EWFRIGISGNKTGISVYLLGIADKGHLAKTYGKTIGKASVTGYC
                     IKFKSISDIKLDVLEKAIRFGMEYGN"
BASE COUNT         1230 a         1333 c         1167 g         1200 t
ORIGIN      
        1 cgagcaggtt gtcgaaataa tgagacggag gaatgccaca tgcacgcaaa gctggcagga
       61 tcagcagcgg gatcagcaac accagcggaa agatcgcgga ccaggtaatc aacacctgtt
      121 tccaacgaac cggcgaggtc gtggaatcgt cctccggtga aaagagaaaa tcgagtccgc
      181 tgtgaatgac atagtgatcg ccttttcgca ggaaaggtct tgctttttcg atcaggcgct
      241 tccgttcatc cgactcgatc cactttcgca ggttcgcttc gctgtcatac cggatgatga
      301 ccgtataagt aaaagtcaga tccttcaccg gccggatgat ttgccagtcg atgagtcctt
      361 cggaacggcg acagatcgga ccgatctcat tcaaccaggc ttcgtactcc gtaatgcgat
      421 cggaaaggac gtgattcgtg atgacgacgg atgctccggt agtttccatt ggcaatgttg
      481 aatggaacaa aggtcacact tcctgaccaa aggtcaagag gttatgatca gggtccagca
      541 gtgagaactc gcgctgcccc cagggtttgt tttcaggttg ttcctttggt aacagcggca
      601 cgcccgcctc ccggaatgaa cggtgcaatt gatcgatatc ggacacccgg atgtaaacct
      661 gaccgtaatt ctccttggga tccaaggaaa cgaattcgaa aaaatgcaat tccaccttct
      721 cccgctccac gatcaggtag tttccgtaat cggctgttac tgtaaagccg agcttctccg
      781 tgtaaaagct acgggtgacg gcaacctgcc gcatgggtag tttagggtga acgcttcgta
      841 acatttccaa cctgatagag ccggtagcag ctaacggttg caagttcgtg atcctggcag
      901 taccatccgc ttccgatacc ttactttttc gcgatcacga tccggaattt gttccggtaa
      961 atgacccgac catccggctt cacaaaaggc tgcatcgatt gccgaagttc cgttcgtacc
     1021 cgttcctcac cggaatgcgc gatggccgcc gccgccggcc cggccgagag gagtccgcga
     1081 agagccgtgt cctgatccgg atagatccat gcgttttccc ggtccacctg atggtgaatg
     1141 gtaaaaccgg cttgcccgat caggttttcc agtttacgat ccgacgtcaa ggcgaaagga
     1201 cccgctcccg ggggcattgt ggacggcatc agtccgccca gtgctttcat aaaagttgcg
     1261 gcttcgcagt cttcccgctc cccccagatc atcgccacca gcgtccctcc gggcttcagt
     1321 acacgccttg cttcggcaaa cgcgttcagc gggttggcgg cgaactgaaa ggaattgaac
     1381 ccacagacca ggtcgaacgc ctcgtcatca aacggcagtc gctccatttc accgacagag
     1441 aaatcaacgc gcgcgtttct ctttcttgcc gcggctatca ggccgtcgct cgcgtccatt
     1501 ccggtaacga tcgcgctatg tgtttgagcg agatggcaga acaggcccga accgcagccg
     1561 atgtcgagca cgcgtttatc cttcagtgcc ggtaaatact ccagcacata gcgataacaa
     1621 tcttccacgg taccttcctg aatagccgcc cagtcatccg ctccgcgact ccagagttgg
     1681 ttttgtgctg cttgcgatcc cattggttag gctatctact atcgtgaaat aagcatgatc
     1741 actaccaaaa tcacccccgc ccctacgaac cacattccct tcttaaacgt cttcgcctga
     1801 ggaggaaaca tccagaagga tgaaacgaca aagaagaaca gcgagcaggc gaaaaagacg
     1861 ttcatgaaaa acaagggatc ggcagtacgc gacgtgtgca ggtcggtcag tttctcgatg
     1921 taagccggcc attccttcgt ggtaaaagtc gcctttccgc tggccttgtc gtactgtccg
     1981 ttatcgaaat agatcgtagt ggaatcttcg cgggtcacct tgagccgacg ctgttcgatc
     2041 gcttcaccca gcacgctacc cgggaggttc ggttctacgg tgcgttccca ggtcttgtcg
     2101 aacttcaaaa aattcgtgtc acgaaaggtc atgatgaagc ccgtgaccgc gtacaccgcc
     2161 atcatgccgg tgagaaaata accgagatag cggtggagaa tgcgcatttg tttcatacgt
     2221 gtactaacaa taaagcaaga tacgaagtgc gattgtcaac agaagtcaaa agcatctgat
     2281 ttgcacatat gacgatgaat gtataatcaa gataatctgc tcaaggttca gctgacatcg
     2341 acaatcctaa tgtacgaacc cccttgaagg taaccggaag aaccaaacag atgttcgcga
     2401 tcttcccgac ctagctagga ggcaaaagca attgtacttc tcttttgaga agaggtaaat
     2461 cacgaatgac aatagaccac aaaatttcag ccgatacact atcatagccg tgcgatatgc
     2521 gatttcgtgt atcaacgatc tttcgtgcgc tggaaatcga aatctcggga cggactctca
     2581 ggatccggct catcgattcg cccataatct caatatttcg ttcgatagca cgcctggttc
     2641 tcaggtcttc ttgaaaggtt aagaagttct tttctacagg aagaaacgct tcgatctccc
     2701 cgatggcgtt caaaatatca gccaaccaag cgttaatttc ctcttccata taccagaact
     2761 ttggtctgat caatgcgctg cttaataaac tgattgctta gtgccttcat ctccagcaaa
     2821 tcaatcggcc tatcaaaaag ttgctgtaaa ttgaacttta aagcaaaata attatcagag
     2881 taaatgattg gatcagaact ttcaaaatcg acaagaaaat ctacatcact tccggctttt
     2941 aggtcgtttc tcactgctga gccaaatgca aacagatacc ggacgtgata ctggtcacac
     3001 aaagcacgaa tttgagatat gttctgcgaa atcaagtcca ttgctcctgc aagataatga
     3061 actatcagca ttcaagcatt tgcttccatg atgttgcggc aagcgattct atccatcaga
     3121 tccgaactgt cggattggta taaaaaatcg agcgattcgt actccaagta cggcacccca
     3181 attcattcga cattaaggcg acacttactg tcaatattcg tacgcggatc gaaccggaaa
     3241 tcaataaatc gtgcttcctt ttgctttccc tccacctcga cggcagcgcg acccatctcc
     3301 ttgtcgctgc tgtcgaatac ccgtgcggtc agcgctccgg aaaaatcctg gttgaagatc
     3361 acgtattgcg acaagaggtt gtcggttccg aggctgtcgc tcgaaaccgt ggactttccc
     3421 agctcaatgc ccttgctctt cagactttcc gacaattcga ctttcacgtc gaacgccttc
     3481 tgaacgccct tcgaagcgcc ttcgatgaac tcgcctgccg tctgcccggc cacatcaccg
     3541 gcctggttga tcttttcctt gatggtggaa gagttgcagg cgaagagtga gaaggcaatc
     3601 gcaaataaga ggaaccttgt gtgtttcatc ataccattag cgacttgaaa gttcagatta
     3661 tttgactcgt tcgtgccaag tcccggaacg aaagttattc atttatggaa actgtgcccg
     3721 acgaaggcca ggttcaagcc ttccgcagta tcttctccat cgtcctgcct ttcgccaatt
     3781 catccaccaa tttgtcgaga tagcggacct gccgcgtaag gggatcttcg atctcctcga
     3841 tccggtagcc gcaaatgagg ccggtgatca gatgagcatt ggggtgcagc ctggcttgtt
     3901 ggaagaacgc ctcaaatgta agcttgtcgt gtatcaccgc atccaaccgc tttccatcat
     3961 aaccggtcag ccagttgatg acctcccgaa gttccgcttc cgtcctgcct ttcctggtta
     4021 ctttctgaag atacaacgga tagaccgacg cgaaagtcat catggcaatg cgctgacgct
     4081 gtttctcgtt ttgttgcata aattacgcta acgaattact aagttaaggg caggatctgg
     4141 attgaaaaag cggcgcgcgc ttgcagttac tatttccaat ctgcgggata tgatctccat
     4201 ggctgtgttc cgaatcgaaa agctattccc gaacaacaaa aacattcgag ttaagaatcg
     4261 aactgctgct ccttacctcg atctggtatc gcccgcgctt gttgatcgct ggaatggacc
     4321 aggttccacc ttccttgaaa ttcgcgtacc ggaccttgca ctggcaacga ggggcggtct
     4381 gggcttccga gtgattcaga aatacggtcc ggcttcgaga tccacggccc ttgcccaggc
     4441 ggtaaacgac ataatagaaa tcacggtcgc tgcaggaaca cgacatggtc ggtatgacgc
     4501 gaaccgttcc aggcttagct tcgctactca accggaccgt aaacccgatc ggttcccggc
     4561 ttgtgtacac ctgcttttcc gtcaaaagcg aatcaacggg catcctgccc tcgaccatca
     4621 gcgggatgat gacgaggctc cagagcaata agcgtagtgg aatccgaagc agcaacagcc
     4681 tgatgctcgt tcgtttcatg ctgacatctt tcaacctagt taccatactc cataccgaat
     4741 cgaatcgcct tttccaatac gtccaatttg atatcgctga tcgatttgaa cttgatgcaa
     4801 tacccggtta cgctggcctt cccgatcgtc tttccgtaag tcttcgcgag atgcccttta
     4861 tccgcaatac cgagtaggta aaccgaaatc cctgtcttgt tgccactgat cccgatccgg
     4921 aaccattccc
//
LOCUS       DAOTON010000091         4918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_14182_length_4918_cov_2.930701, whole genome shotgun sequence.
ACCESSION   DAOTON010000091 DAOTON010000000
VERSION     DAOTON010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14182_length_4918_cov_2.930701"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="P5292_02835"
     CDS             complement(<1..1437)
                     /locus_tag="P5292_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38092.1"
                     /translation="MILFLLFFASMGVMAQRTGYVEVTGKVRKDNKPVGGVSIAVNCP
                     GIPPQTITSQANGTFSFRMELQHDYTINFSQYGLVPKSVIFSTKVPADQADIIFQNEF
                     NIDMFEDVAGVSQNASMNKPVARIAFNPTYEDFVHDASYTKQIQAEQDLARRQAEELR
                     RQQERARLDSLNKVWNDSLAKAKDREAQLMAQRAEQDRLKKEQERARLDSLARVQAAA
                     SAAMREQARLDSLSAAEAKAKALAEAREKARQDSIAKADAEQARLAEQARLREQARLE
                     AEAKVAEKARQDSIVAAEAAERARLKAEADAIAKAEADAKARAIAEEARKKQVADSLA
                     KAEEARLRAEAAARAKAEADARAQAEAAKAKEKARQDSIVAAENAERARLKAEADAKL
                     KADAEAKLRAEAEAKAREKARQDSIARAEQEARDRVLAEEKARQEKIQKDKEAAEAKR
                     LAELAEQTRRDSLAKAEADRVKREAEAKA"
     gene            complement(1629..2486)
                     /locus_tag="P5292_02840"
     CDS             complement(1629..2486)
                     /locus_tag="P5292_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRS38093.1"
                     /translation="MTSTIQLTECPRDAMQGWPEFIPTDVKARYLQALLEVGFDTLDF
                     GSFVSPKAIPQLRDTSEVLSKLDLRTTTTSLLAIVANVRGAEDACKHEPIRYLGFPFS
                     ISEEFQRRNTNATIAESLERVREIQSLAKTAGKDVLIYISMGFGNPYGEAWSPELAVQ
                     WVQRLADEGIRRIALADTVGVSNPENIRALFSHLIPSVPEVTFGAHLHSHPATWEEKI
                     TAAYESGCRNFDLALKGIGGCPMANNDLVGNIATEQVVTKFGGPSAMGLNAEAWSRAV
                     AIAGEIFHA"
     gene            complement(2491..2994)
                     /locus_tag="P5292_02845"
     CDS             complement(2491..2994)
                     /locus_tag="P5292_02845"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRS38094.1"
                     /translation="MRPLLLLLFCLIIGEARAQTDASGYTVLAEGTIAPDIKGIDQQG
                     KPVCLSKVKATYVAVFFYEPGCHFCEYILPDLQRFYQREHGANLEIVAVALTTDAAAW
                     KEQLAAHTAGWTELLPADVNKVRQDYRIEVAPTIYLIDRKHRVRSTRLVRSDDLELRW
                     FELFNEL"
     gene            complement(3001..3462)
                     /locus_tag="P5292_02850"
     CDS             complement(3001..3462)
                     /locus_tag="P5292_02850"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HRS38095.1"
                     /translation="MKSRITLLLIATVLTLSFSCKNVNQPTSFGGPVDEKVAVPAADA
                     KNLLTDKPEATATLAGKVNEVCQAEGCWFNLDQGSGVALLVRMPEHSFTVPKDLSGKQ
                     VVVAGRLHYDTTDVATLREYAKEDGKSEEEINAINEPSVELVLEASGVKIQ"
     gene            3600..4280
                     /locus_tag="P5292_02855"
     CDS             3600..4280
                     /locus_tag="P5292_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS38096.1"
                     /translation="MAETQQEQEISVQEAIGRTEHFINQNRKSLGIIIGALVVAVGGY
                     LFYQKVYVAGKEKEASTALFHAEAYFRQDSLRLAINGDGNNMGLEEIVNQYSVSPSGN
                     LARYYLGMAYLRTGAFDKAVESLKSYDAKDETTGSLAYGAIGDAYMELNNADEAISFY
                     EKASKEKPNNFTTPIMLMKLAGALEGKSNFKEAKEVYERLKKDYPASTEGTQAEKYIS
                     RAEAMIGK"
     gene            4343..4837
                     /gene="ribH"
                     /locus_tag="P5292_02860"
     CDS             4343..4837
                     /gene="ribH"
                     /locus_tag="P5292_02860"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRS38097.1"
                     /translation="MATHLKNLSDFNAAELPSAKGMRFALVVSSWNDKISGALADGAR
                     EALLAAGCSAEDIERVDVPGSYELPLGARWMADQQRYDAILCLGCVIQGETRHFDFIC
                     DAVANGLMDLNLEYGLPFIFGVLTPNTLEQAIDRAGGKHGNKGVEAATTAIRMVALKR
                     SINK"
BASE COUNT         1050 a         1401 c         1313 g         1154 t
ORIGIN      
        1 ggccttggct tccgcttccc gcttgactcg gtcggcttcg gctttcgcaa gggagtcgcg
       61 acgggtttgc tcggcgagtt ctgccaggcg cttcgcttca gcagcttcct tatccttttg
      121 gatcttctct tgccgcgcct tctcttcggc cagtacacgg tcacgtgctt cctgctcggc
      181 tctggcgatc gaatcctgac gcgccttttc acgagccttc gcttctgctt ccgctctcag
      241 cttagcttcc gcgtcggctt tgagtttcgc gtcggcttct gctttcaggc gagcgcgttc
      301 agcattttcc gcggctacga ttgaatcctg acgtgctttt tcctttgctt tagctgcttc
      361 tgcctgcgca cgcgcatccg cctctgcctt ggctcttgcg gcggcctcgg cgcgaagacg
      421 agcttcttca gctttggcca gcgaatccgc tacttgtttc ttccgggctt cctccgcgat
      481 ggctcttgct ttggcatccg cctcggcttt tgcaatggca tccgcctcgg ccttcagcct
      541 tgcccgctca gcggcttcgg ccgcaacgat tgaatcttgt cttgctttct cggccacttt
      601 cgcttccgcc tccagacggg cctgctcgcg caagcgggct tgctccgcta aacgggcctg
      661 ttcggcatcg gctttggcga tggaatcctg tcgggctttt tctcttgcct ctgcgagcgc
      721 tttcgccttt gcttcggcgg cggaaagact gtcgaggcgc gcttgttcgc gcatagctgc
      781 cgacgcagcc gcctggacac gggccaacga atcaaggcgc gcacgctcct gctccttctt
      841 taaccggtcc tgctcggccc gttgtgccat caactgggcc tcccgatcct tggccttggc
      901 cagcgagtca ttccaaacct tattcagaga atccaggcga gcacgctctt gttgacgccg
      961 aagttcttcc gcctgccggc gtgcaagatc ctgttcggcc tggatctgct tcgtgtaact
     1021 ggcgtcatgg acaaaatcct cataagtagg attaaaggca atccgggcaa ccggtttgtt
     1081 catggatgca ttctgagaaa ctcccgctac atcctcgaac atatcgatgt tgaactcgtt
     1141 ctgaaaaatg atgtcggcct gatcggccgg cactttggta ctaaaaatga cggatttggg
     1201 cacaaggccg tactgggaga aattgatggt gtaatcgtgc tgtaactcca tgcggaagga
     1261 gaaggtccca ttcgcctgcg aggtgatggt ctgtggggga atgcccggac aattaacggc
     1321 aatcgaaacg ccgccgacgg gcttattgtc cttccgcacc tttccggtga cctccacgta
     1381 cccggtacgt tgggccataa cgcccatgga agcgaagaaa aggaggaata aaatcaccaa
     1441 ccggaagtac cggcttcctg gggcaggaat cgtttgaatg ttacgcacgc agactgattc
     1501 gaattaaggt ataacaaagg tacggagtag aaccccgttt attgtatgaa tttcagactt
     1561 ttccggagaa atttgcacac gtttctccgg tattcaaata ccgtacctgt acaagaaagt
     1621 ttcagtaatc aggcgtgaaa aatctcaccg gctatagcta cggccctgga ccaggcttcc
     1681 gcattcagcc ccatggctga cggcccgccg aatttcgtca ccacctgctc ggtggcaata
     1741 tttccgacca gatcattgtt ggccatcggg cagccgccga tacccttcag ggccagatcg
     1801 aaattacggc agccgctttc ataggcggct gtaattttct cttcccaggt ggcaggatgc
     1861 gaatgaagat gcgctccgaa ggtcacttcc ggcaccgaag gaatcaggtg ggaaaacaag
     1921 gcccggatgt tttccggatt actgaccccg acggtgtcag ccaaggcgat cctccggatg
     1981 ccttcatcgg ccagtctctg cacccactga accgccagtt cggggctcca ggcttcgcca
     2041 tacggattgc cgaaacccat cgagatgtag atgagcacat ctttccctgc cgtcttcgcc
     2101 agcgattgga tctcccgtac ccgctccaac gactctgcga tggtcgcatt cgtattcctg
     2161 cgttggaatt cctcgctgat cgaaaaagga aaacccaggt aacggatcgg ctcgtgctta
     2221 caggcatcct ccgcgccgcg tacattggcc acgatggcca gcagggaggt cgtggtagtc
     2281 cgcagatcga gtttcgataa tacttcggaa gtatcccgca attgcggaat ggctttgggc
     2341 gaaacgaagc tgccaaaatc gagtgtatca aaaccgactt ccaacagggc ttgcaggtaa
     2401 cgagccttca catcggtcgg aataaactcc ggccaaccct gcatggcatc ccgcggacat
     2461 tcggtgagct gaatcgttga cgtcatcgga tcataattca ttgaagagtt cgaaccagcg
     2521 caattccagg tcatcgcttc tgacaaggcg tgtactacga acccggtgtt tccggtcgat
     2581 caggtagatg gtcggggcta cttctattcg atagtcctgt ctgaccttgt ttacgtcagc
     2641 tggcagtagt tctgtccagc cggcggtgtg ggcagccagt tgttccttcc aggcggctgc
     2701 atccgtcgtc agggccaccg cgacgatttc gagattggct ccatgctcac gctggtaaaa
     2761 gcgctgcaag tcgggcaaga tgtattcgca aaaatggcaa ccgggctcat agaagaagac
     2821 ggctacataa gtcgccttga cctttgaaag gcaaacgggc tttccctgct gatcgatgcc
     2881 tttgatgtcg ggagcgatcg tcccttcagc caaaacggta tatccgctcg catccgtttg
     2941 ggcgcgagct tctccgatga tcaggcagaa gagcagcagg agaagtggcc gcatggaggt
     3001 ttattggatc ttcactccgc tcgcctcgag gaccaattcc acggaaggtt cgttgatcgc
     3061 gttgatctcc tcctccgact ttccgtcttc tttggcatac tcccgcaggg tggccacatc
     3121 ggtggtgtcg taatgcaaac gaccggctac gaccacttgc tttcctgaaa ggtctttcgg
     3181 cacggtgaaa ctgtgttcgg gcatacgcac cagcagtgcg acaccgcttc cctgatcgag
     3241 gttgaaccag cagccttccg cctggcagac ctcattcacc ttaccggcaa gtgtcgcggt
     3301 ggcttccggt ttgtccgtca acaggttctt cgcgtcggca gccggcaccg caaccttctc
     3361 atcgaccggt ccgccaaagc tggtcggctg gttgacattt ttgcaggaaa aagacagggt
     3421 caggaccgtg gctatcagca gcagggtgat tctggatttc ataactgcaa aggtacatcc
     3481 tgggagtcga tttgtggatt tgctcaaaaa cgtgttgaaa acacggattt cagcatcctg
     3541 aatccttgga atagccacaa aaaaaggtta tttttgcagc cttgtttatt tgaacaaaaa
     3601 tggctgaaac tcaacaagaa caggaaatca gcgtccagga agcgatcggg cgcacggagc
     3661 atttcatcaa ccagaatcgc aagagcctgg gtatcatcat cggtgccctg gtcgtagcgg
     3721 tcggcggtta cctgttttat cagaaagtat acgttgcagg caaggagaaa gaagcatcca
     3781 ccgccctctt ccatgctgaa gcgtatttcc gtcaggactc cctccgtctt gctatcaatg
     3841 gcgatggcaa caacatgggc ctggaggaga tcgtgaacca atacagtgtc agcccctccg
     3901 gcaacctggc tcgctactac ctcggcatgg cttatctgcg caccggcgct ttcgacaagg
     3961 ccgtagagtc gttgaagagc tacgatgcga aagacgagac taccggttcg ctcgcttatg
     4021 gtgcaatcgg tgatgcctat atggaattga acaacgccga cgaagccatt tcgttctacg
     4081 aaaaggcttc caaggaaaag cctaacaact tcaccacccc gatcatgctg atgaagctgg
     4141 ccggcgcgct ggaaggtaaa tccaatttca aggaagcgaa ggaagtatac gaacgcctca
     4201 agaaggacta tcctgccagc accgaaggca cacaggccga gaaatacatc tcccgtgccg
     4261 aggcgatgat cggtaaataa ccctaaacga acgcatgaaa aaggctgccc gaacggcagc
     4321 ctttttcata tccgcagcat ccatggctac ccatctgaag aacctgtctg atttcaacgc
     4381 ggccgaactt ccttccgcga agggaatgcg tttcgcgctg gtcgtttctt cctggaatga
     4441 caagatcagc ggcgcactcg ccgatggagc ccgggaagcg ctcttagccg ctggttgttc
     4501 ggcggaggat atcgaacggg tcgatgtacc gggaagctat gagttaccct tgggagcccg
     4561 ctggatggcc gaccagcagc gctacgacgc catcctttgc ctgggctgtg tgatccaggg
     4621 agaaacccgg cactttgatt tcatctgcga tgccgttgcg aacggattga tggacctgaa
     4681 tctcgagtac ggactcccct tcattttcgg cgtgttgaca cccaataccc tggagcaagc
     4741 catcgaccgg gctggcggta aacacgggaa caaaggtgtt gaggcggcaa ccacggctat
     4801 ccgcatggta gccctgaagc gatccatcaa caaataaaaa aggcggaaag aacatcttcc
     4861 cgcctcccta aaactcttgc ctgggtcagt cgcgcacttg aatgttcgta acacgatc
//
LOCUS       DAOTON010000092         4881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_14306_length_4881_cov_2.932864, whole genome shotgun sequence.
ACCESSION   DAOTON010000092 DAOTON010000000
VERSION     DAOTON010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14306_length_4881_cov_2.932864"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1414)
                     /locus_tag="P5292_02865"
     CDS             complement(20..1414)
                     /locus_tag="P5292_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38098.1"
                     /translation="MIKKVLLPLLLLFSCSQVVVADEPNQTSTETEKSRPVRLPTLGI
                     SHGVMSFIGDIGYHRFNEPLWNRSGLQFDLQYPLLPRFSVSAFLLSGRIGSNDYVPER
                     PLNFRASIVSEGVLLRYEWISNKMNQQILIPYITGGIGYTFFMSKADLKDGQGRTYHY
                     WSDGSIRDLAEDAPNAADAVRIYRDYDYETDLRDANLDGFGKYRPGTLTIPAGAGVRM
                     RISGRCSIQLGTILHLYRTDLLESVNAESAGSRKGNSDNDKLVFTSAGFRYDFSGPRE
                     NSKKAKGYRVRKEDVKNIDFNALLSEDADGDGVPDVRDDSAASPEVARKNVDVSGKPV
                     DTDYDGIPDYRDKEPNSGRDAVVTEEGITITQQMVEEKFRRDSLEALPAVREYLQSFD
                     RLTQRKAAAGQPPQPQDRTFIPSQYLRLDRDLNGTISPNEVSAAIDEYMEGKSPYTVA
                     EFYQLIDYFFGQRR"
     gene            complement(1494..2684)
                     /locus_tag="P5292_02870"
     CDS             complement(1494..2684)
                     /locus_tag="P5292_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38099.1"
                     /translation="MFKLLSTFLSASVLFVLSYFFSPEAGPQVSIDAPAQIRPGESAE
                     VRLTVEKGSMTGFARLQVFLPEGLTADGGALQNSQFLHEDNFIKFIWIDLPTDSIFTV
                     SLFLHVAPDAQGTKYLNGFFSYLEEEVSKKITFQETAIEISPNAMATTLPKPDVTRRI
                     IANNPENGSYRVELDIRPNSGQLSARFIDQLPAGFTAEAIESKGAEFSQDAEKVIFRW
                     TALPQDSLFTISYQATGPVTAGAPEISGMLVFGTEAIDLNDATAATDTEPADLIVEEL
                     LATTAAKESNPTASSAPADISVPAPQAGLYFTVQVAATNRSPKRDNAWFESRFHFNRQ
                     VSRTEHEGWNKYLIGQFPSYEEARSVRDKARGEVTDAFVVAYDENGNRIPVGQALSRK
                     PLNQ"
     gene            complement(2831..3538)
                     /locus_tag="P5292_02875"
     CDS             complement(2831..3538)
                     /locus_tag="P5292_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRS38100.1"
                     /translation="MNLDELKIRKAFSDKDWQEIKAFDTWQIFKIMAEFVDGFEKLSK
                     IGPCVSIFGSARTKPEHPYYQLAEEIAYKLTKEGYGIITGGGPGIMEAANKGARTAGG
                     KSVGLNIELPFEQSSNPYIDRDKLITFDYFFVRKLMFIKYAQGFVVLPGGFGTMDELF
                     EALTLIQTKKIGKFPIVLVDKDYWQGLMDWVTKTFIKEGMIDKNDMNLFKVADSADEA
                     VQHINEFYSRYLLKPNF"
     gene            complement(3680..>4881)
                     /locus_tag="P5292_02880"
     CDS             complement(3680..>4881)
                     /locus_tag="P5292_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRS38101.1"
                     /translation="ETILAADHILDLGPGAGVHGGRVVAEGTPESILQQSSLTADYLN
                     GRRQIALPEKPRKGNGKHLELKGATGNNLKKVSVRIPLGKLVVVTGVSGSGKSTLINE
                     TLYPILNQHFFNALQKPLPYQSIKGLEHIDKVIEIDQSPIGRTPRSNPATYTGVFTDI
                     RNLFASLPEAAIRGYAPGRFSFNVKGGRCETCQGGGMRVIEMNFLPDVYVECETCHGK
                     RYNRETLEIRYRGKSISDVLDMSIEQAVDFFSNLPHIVHKIKTLQEVGLGYITLGQQS
                     TTLSGGEAQRVKLATELSKRDTGNTFYILDEPTTGLHFEDVRILLEVLNRLVEKGNSV
                     LVIEHNMDVIKVADHIIDLGPEGGERGGTIICEGNPQEVAKSQESVTAQFLRKELEEM
                     AQLAARR"
BASE COUNT         1047 a         1287 c         1327 g         1220 t
ORIGIN      
        1 actgcctact gccaaccgcc tatcgtcgtt gaccgaagaa ataatcaatc aactgataaa
       61 attccgccac cgtatacggc gatttgcctt ccatgtactc gtcgatcgct gcacttactt
      121 cattcggtga aatggtgccg ttcaggtcgc ggtcgagtcg gagatactgc gatgggataa
      181 aggtccggtc ttgcggctga ggcggctgac ctgccgctgc cttgcgctgt gtcagacggt
      241 caaagctctg tagatattca cgaacagcag gcagtgcttc cagtgagtcg cgacggaatt
      301 tctcttccac catttgttgg gtgatggtaa tgccttcctc cgttaccacc gcgtcgcggc
      361 cactgttcgg ctctttgtcg cggtaatcgg ggatgccgtc ataatcagta tccaccggtt
      421 tgccagacac atccacgttc ttgcgtgcaa cttccggcga tgcggccgaa tcatcccgaa
      481 catccggtac tccgtccccg tctgcgtctt ccgacaacaa agcgttgaag tcgatgttct
      541 tcacgtcctc cttccgcacc cgataacctt tggccttctt actgttttcc cgcggacccg
      601 agaaatcgta acgaaagccc gcggaggtga agacgagttt gtcgttatcc gaatttcctt
      661 tgcggctgcc ggcactttcc gcgttgacgg attccaacag atcggtccgg tagaggtgaa
      721 ggatcgtacc cagttgaatg gagcaacgtc cggaaattcg catccggaca cccgctcccg
      781 ctggtatcgt caatgtgcct gggcgatact tcccaaaacc gtcgaggttg gcgtcgcgca
      841 ggtccgtttc gtagtcataa tcccggtaga tcctgaccgc atccgcagca ttcggagcat
      901 cttccgcgag gtcgcggatg gaaccgtcgc tccagtagtg atacgtgcgt ccctggccgt
      961 ccttcagatc ggctttggac atgaagaagg tgtaaccgat cccgcccgta atgtacggga
     1021 tgaggatctg ttggttcatt ttattgctga tccattcgta gcgcaacaat acgccttccg
     1081 aaacgatgga tgcacggaag ttcaacgggc gttccgggac ataatcattc gacccgatac
     1141 ggccggaaag aagaaaggct gaaacactga aacggggcag gaggggatac tgcaggtcga
     1201 attgcaaacc ggaacgattc cataacggct cattgaaacg atggtaaccg atatcgccga
     1261 tgaaactcat cacaccatga ctgatgccca gtgtcggcaa acgtaccgga cggctttttt
     1321 cggtctcggt actcgtctga ttcggttcat ccgcaacgac gacttgtgag caactgaaaa
     1381 gcaggagaag cggtagcagt actttcttga tcatggtgtc ggattgcatg cacgtaggtg
     1441 ccggccggtg gcgatgtcca gtggagtggg tagaatgatc cctgttatac tgcttactga
     1501 ttcaagggtt tccggctgag ggcctggcct acgggaatgc ggttcccgtt ttcatcgtaa
     1561 gccaccacaa aggcatccgt gacttcacca cgcgccttat cccgaacgga acgggcttct
     1621 tcataggaag gaaactgtcc gatcaggtac ttgttccagc cctcatgctc ggttcggctg
     1681 acctggcggt tgaagtgaaa acggctctcg aaccaggcgt tatcacgctt cggactgcgg
     1741 ttcgtggcag cgacctgaac ggtgaagtac aatccggctt ggggcgcagg aacggaaatg
     1801 tcggcaggtg ctgatgaggc tgtcgggttg ctttctttcg ctgctgtggt agcaagcagt
     1861 tcttccacga tcaggtcggc aggttcggta tccgtggccg cggtggcgtc attcaagtcg
     1921 atggcttcgg ttccaaaaac cagcataccg gaaatttccg gggcaccggc tgtaacgggg
     1981 cctgtcgcct gataactgat cgtgaacagg gaatcctgcg ggagtgccgt ccaacggaag
     2041 atcacttttt ccgcatcctg gctgaactcc gcgcccttgg actcgatcgc ttcggccgtg
     2101 aatcctgccg gaagctgatc gatgaaacgg gccgatagtt gtccgctgtt gggacggatg
     2161 tcaagctcaa cgcgatagga accgttttcg ggattgttgg caatgatccg acgggtaaca
     2221 tcgggtttcg gaagggtagt ggccattgcg ttcgggctga tctcgatcgc ggtttcctgg
     2281 aaggtgattt ttttggaaac ttcttcttcc agataagaga agaacccgtt caggtatttg
     2341 gttccctgtg cgtccggtgc aacatgcagg aacagactga ccgtgaatat cgaatctgtc
     2401 ggcagatcga tccagatgaa cttgatgaaa ttgtcttcgt gcaggaactg ggagttctgc
     2461 aaggcgccgc cgtcagccgt cagtccttcc ggaagaaaga cttgcagtcg ggcaaaaccg
     2521 gtcatggaac ctttttcgac ggtcagcctg acttccgccg attcacccgg acggatctgt
     2581 gccggcgcgt caatgctgac ctgtggcccc gcttccggac tgaaaaaata ggataagacg
     2641 aagagaacag aggcggagag gaaggtgctg agcagtttga acatgatata cgcttttgcg
     2701 tcgtatcaaa gtactggctt tcagctcaaa tcggatggtt tgtctgctta cagttatcca
     2761 tcccgccatg ttaaaaacca aaggccgtga aatgcatctc acggccttcg ttacgactga
     2821 aaaatcgcac tcagaagttg ggtttcaaca ggtagcgcga gtagaattca ttgatatgct
     2881 gaaccgcctc atccgcagaa tcggcgacct tgaacaggtt catgtcattc ttatcgatca
     2941 tgccctcctt gatgaaggtt ttagttaccc aatccatcaa tccctgccag tagtccttgt
     3001 cgaccagtac gatcgggaac ttaccgatct ttttggtttg tatcagggtc aaggcctcaa
     3061 agagttcatc catcgttccg aaacctcccg gcaagacgac gaatccctgg gcgtatttga
     3121 tgaacatcag tttccggaca aagaagtaat cgaaggtgat gagtttgtcc cggtcgatat
     3181 acggattgga actttgttcg aatggcagtt cgatgttaag cccgacggat ttcccgcccg
     3241 cagtacgcgc tcccttattg gccgcttcca tgatgccggg tccgccaccg gtgatgatgc
     3301 cgtatccttc tttcgtgagt ttgtaggcga tctcctctgc cagttggtag tagggatgct
     3361 ccggttttgt ccgggcggaa ccgaagatgg aaacacacgg cccgatcttg ctcagctttt
     3421 cgaatccgtc cacgaattcg gccatgatct tgaagatctg ccaggtatca aatgctttga
     3481 tttcctgcca gtctttgtcc gaaaaagctt tcctgatttt taattcatcc agattcataa
     3541 taatacgtta gttcaacaca ggcgtttgta ggtaacaatc gccgcgggag agggtttagc
     3601 cctgttaata cgatggcgaa caaagaaggt tgccggggcg aaattgcctt gtaataagga
     3661 agttcagccg gttctcggat caacgccttg cagccagctg ggccatttct tccagttcct
     3721 tgcgcaggaa ctgggcggtc acactctctt gcgatttggc cacttcctgc ggattgcctt
     3781 cacaaatgat ggtgccgcct cgctcaccgc cctccggacc gagatcaatg atgtgatcgg
     3841 ctactttgat cacgtccatg ttatgttcga tcaccagcac gctgttgcct ttttctacca
     3901 gtcggttcag tacctccagc agaatgcgga catcttcgaa gtgcaaaccg gtcgtcggtt
     3961 cgtccaggat gtaaaatgta ttgccggtgt cacgcttgga taattcagta gcgagtttga
     4021 cacgctgtgc ttccccgccc gatagcgtgg tgctctgttg tccgagcgtg atgtaaccca
     4081 gtccgacttc ctggagcgtc ttgatcttgt gtacgatgtg tggcaggttg gaaaagaagt
     4141 cgacggcctg ctcgatactc atatccagta catcgctgat cgacttgccc cggtaacgta
     4201 tttccagtgt ttcccggttg tagcgtttcc catgacaggt ttcgcattcc acgtaaacat
     4261 caggaagaaa gttcatctcg atgacccgca tgccaccacc ctgacaggtc tcacagcggc
     4321 ctccttttac attgaaggaa aaccgtccgg gtgcgtagcc gcgaattgct gcttccggaa
     4381 gcgaagcgaa cagattgcgg atgtcggtaa agactccggt ataggtggcg ggattcgaac
     4441 gcggtgtccg cccgatcggc gattggtcga tttcaatgac cttgtcgata tgctccaatc
     4501 ctttgatgct ctggtagggg agcggcttct gcagcgcgtt gaaaaaatgc tggttcagga
     4561 tgggatataa ggtctcgttg atgagtgtcg acttaccgct gcccgatacg ccggtcacaa
     4621 ccaccagctt ccccaggggg atcctgacac tgactttttt cagattgttg cccgttgctc
     4681 ccttcagctc aaggtgcttg ccgttacctt ttcggggctt ttccggcagc gcgatttgac
     4741 gacggccatt caggtagtcg gcggtcagac tgctctgttg cagaatactt tcaggagttc
     4801 cttcggcaac gacgcggccg ccgtgcacgc cggcacccgg accaagatcc aggatatgat
     4861 cggccgcaag gatggtttcc t
//
LOCUS       DAOTON010000093         4875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_14333_length_4875_cov_3.193154, whole genome shotgun sequence.
ACCESSION   DAOTON010000093 DAOTON010000000
VERSION     DAOTON010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14333_length_4875_cov_3.193154"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..1094
                     /locus_tag="P5292_02885"
                     /pseudo
     CDS             13..1094
                     /locus_tag="P5292_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            1266..3527
                     /locus_tag="P5292_02890"
     CDS             1266..3527
                     /locus_tag="P5292_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRS38102.1"
                     /translation="MPADKYLLSQNIIKSDTTILEKDKILSLVKQQPNRLILGFLPFH
                     LTVYNIGNTGKPNRFKNWLKSIGEEPAVLDPLLTNRSRNQINLLLLQNGFFNSNVTDS
                     TSYHKRRARVYYSISYGDAYRLRKVDFSTRDTALQQYLELIRTTTLLKPETRYDEELF
                     EEERERVTSELKDRGFYQFSKNYISFSADTTVGAHRVDLYVYINRINENVDPALTNEG
                     PKRSHAVYFLRNIYIQTDYDPKNPNAAAPVDTQLVNGYYLLGNHPQHFIRDQTLLDCI
                     FVKSGDRYLQRDFDYSYRRLQSLNVFKFVNMLFREVPDDSSGRRQLDVIIQLTPLDKQ
                     DFTLETEATHTGGNLGIAGSFGYRNKNVFRGAEVFEFRIKGALEALPNFSDSLETKRL
                     LFFNTYEIGPEVSLSFKKFLLPGFITRSTSRYFNPRSTLTTGFNYQDRPDFRRSILNL
                     SFGYQWRGSTTQRFSVFPFELNSVYVKPSPGFQARLDSLQDPRLRYSYENHLITAARF
                     SWLYTDQEIRPNQSFWFLRANFEMSGLVMRGIAPLFNWERNEEGSYVFSGIKFSQYIK
                     PDVDVSYHHILNKNNTLVYRVATGMGLPYGNSRALPFEKSFFAGGASSIRAWSARTLG
                     PGSYNESLNIEQSGDFKFEANVEYRSFLLQLFGSTILEGAAFVDAGNVWTRNEDASRP
                     GSQFRISTFARELGIGGGLGMRFNFSFFILRLDMAVKLHDPSLDLSERWVYPNQKFVI
                     GDIVPNLAIGYPF"
     gene            3785..4843
                     /locus_tag="P5292_02895"
     CDS             3785..4843
                     /locus_tag="P5292_02895"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HRS38103.1"
                     /translation="MSSAKIAELLGAGAESLLNHQSKTISKEHLHLPGPDFLDRIFAP
                     TNRSNQVIRSLQALYGHGRLKDTGYVSILPVDQGIEHSAGASFAPNPIYFDPENIVKL
                     AIEGGCNAVASTYGVLAAVSRKYAHKIPFIVKINHNEFLSYPNKFDQIMFGTVQEAWN
                     MGAVAVGATIYFGSDESARQIQEVSEAFEYAHELGMATILWCYLRNPGFKKDGKDYHT
                     AADLTGQANHLGVTIQADIIKQKLPTNNGGYTAVAFGKTHPKVYSELSSDHPIDLCRY
                     QVANCYMGRMGLINSGGESKGASDLSEAVTTAVINKRAGGQGLISGRKAFQKPMTEGV
                     ALLNAIQDVYLSKDITIA"
BASE COUNT         1127 a         1461 c         1222 g         1065 t
ORIGIN      
        1 gatgtcctca tcatcggcag cgggatcgtc gggctgaatg ccgctttgta cctgaaacgg
       61 aaatcccccg aactcaccgt caccatcatc gaccgggaat cctgcccttc ggagcatcca
      121 cgcgcaacgc aggctttgcc tgcttcggta gtccgtccga gatactggcc gatctgaaaa
      181 agcagaacga agatgcggtc ttctcgctgg tcgaacgccg ctggaaagga ctactgcggt
      241 tacgggaaaa tctcggcgac gcggcgattg cttatgaagc cttcggcagc cacgaagtat
      301 tccgcaagga agacgaagtc ctttatcaga cttgccttga gcagctcccc tatctgaatc
      361 gtcaactgaa gaccatcaca ggcaccgacg agaactacgg catcagcagc gaacgcatca
      421 gcgatttcgg cttccggaac atccggcaca tgttgttcaa caggagcgaa ggtcagctcg
      481 acaccggccg gatgatcgaa gcgctcttgc acaaagtgcg cgggcttggt gtaaggatcc
      541 tgaacggact caccgtggag gcgatagaat caaacagcag tggcgccgtc gtgatgtgca
      601 aagatgacat tcgcttttcc gcacgccagg tgttgctcgc taccaacgga ttcaccaaaa
      661 cgctgttacc ggaagtggac gtacagcctg cgcgtgccca ggtgttcgtg acggatccga
      721 ttcccggtct accgtttcgc ggctgctttc actacgacga agggttctac tatttccgca
      781 acatcgggga tcgtatcctg cttggcggcg gccgcaacct caacatcgcg ggcgaaacga
      841 cgatggacat gaccctgaca gaccagatcc aaagcgcact ggaccgttta ttgcacgaaa
      901 ccattgcacc cgggaaaaac gcgaaagtcg cgatgcgctg gagcggtatc atgggcatcg
      961 gcgaagtgaa agctccgatt ctgcagcaag tcaggcccgc tgtatatacc gctgtcagaa
     1021 tgggtggaat gggtgtagcc ctggcaagcc ttgtcggcga agaagccgcg gatatgctgc
     1081 tcggggaccg ctgagcttat tcaccaggtc gtgttcaacg cccgttgtcc gagcaatgct
     1141 caggggccta attatgctat ttttgcaccg atacgcaggc ctcaccagtg ctccactcct
     1201 ttcggcgtct agttcttctt ttcattgcta tcagtgcgct ggcggcgtgt cgaccaacgc
     1261 tttacgtgcc tgccgataaa tacctgctct cccagaatat catcaagtcg gatacgacga
     1321 tcctggagaa agataagatc ctctcgctgg tcaagcagca accgaaccgg ctgatccttg
     1381 gcttcctgcc gtttcacctg acggtttaca acatcggcaa caccgggaag ccgaaccgat
     1441 tcaagaactg gctgaaaagc atcggagaag aaccggctgt gctcgatcct ctgctgacca
     1501 atcgctcccg gaaccagatc aacctcttgc tgctgcaaaa cggtttcttc aattccaacg
     1561 tcaccgattc caccagttac cacaagcgaa gagcccgggt ctattattcg atcagttatg
     1621 gtgacgccta tcgcctgagg aaagtcgatt tctcgacacg cgataccgcc ttgcagcaat
     1681 acctcgagtt gatccggacc accaccctgt tgaagcccga gacacgctac gacgaagaac
     1741 tgttcgaaga ggaacgggaa cgcgtcacct ccgagttgaa ggaccgcggt ttctaccagt
     1801 tcagcaagaa ttacatctcc ttcagtgcag acacgacggt tggcgcgcac agggtcgatc
     1861 tgtacgtgta catcaaccgg atcaacgaaa acgtggatcc ggcgctgacg aacgaaggac
     1921 cgaaacgctc acacgcggtc tatttcctgc ggaacatata catacaaacc gactacgatc
     1981 cgaagaatcc aaacgcggct gctccggttg acacccaatt ggtcaatggc tattacctgt
     2041 taggcaatca tccgcagcac ttcatccgcg accagaccct gctcgattgc atcttcgtaa
     2101 aaagcggcga tcgctacctg caacgcgact tcgattactc ctatcgccgt ttacaatcgt
     2161 tgaacgtgtt caagttcgtg aacatgctct tccgtgaagt ccccgacgac agttccggtc
     2221 gtcgtcaact cgatgtgatc atccagttga ctccgctcga caagcaggac ttcacgctcg
     2281 aaacggaggc cacgcatacc ggtggcaacc tgggaattgc gggatccttc ggttaccgga
     2341 acaagaacgt tttccgcgga gccgaagtgt ttgaattccg gataaagggc gcgctcgaag
     2401 cccttcccaa cttctccgac agcctcgaaa ccaagcgact cctgttcttc aacacctacg
     2461 agatcgggcc ggaagtgtcg ctgtctttca agaaattcct gttgccgggt ttcattacac
     2521 gtagcacttc ccgttacttc aatccgaggt cgacgctaac aaccggattc aattaccagg
     2581 accgtcccga cttccgccgg tccatcctta atctctcctt tggctatcaa tggcgcggat
     2641 caacaacaca acggttctcc gtttttccgt tcgaactgaa ctccgtttat gtaaaaccga
     2701 gtccgggttt ccaggctcga ctcgacagcc tgcaggaccc gcgcctacgg tacagctacg
     2761 aaaatcacct catcacggcc gcgcgctttt cctggttgta caccgaccag gagatccggc
     2821 ccaaccagag tttttggttc ctgcgtgcga actttgaaat gtcggggctg gtcatgcgcg
     2881 gcatcgcgcc cttgttcaac tgggaacgga acgaagaagg cagctatgta ttttccggca
     2941 tcaagttttc gcagtacata aagccggatg tggatgtgag ctatcaccac atcctcaaca
     3001 agaacaacac cctcgtttac cgcgttgcaa ccggcatggg cttgccgtac gggaactccc
     3061 gggcgttgcc gtttgaaaaa agcttcttcg ccggcggtgc cagcagcatc agagcctgga
     3121 gcgcgcgtac actgggtccg ggctcttaca atgaatcgct caacatcgaa cagagcggtg
     3181 atttcaagtt cgaagccaat gtcgagtacc gttccttcct cctccaactc ttcggctcca
     3241 ccatattaga aggtgcggca ttcgtcgatg ccggcaacgt ctggacacga aacgaagacg
     3301 cgagccggcc ggggtcgcag ttccgcatct ccaccttcgc gcgcgagctc ggcatcggcg
     3361 gcggattggg catgcgcttc aacttttcct tcttcatcct ccgcctcgac atggccgtca
     3421 aactgcacga cccatccctc gacctgtccg aacgctgggt ctatcccaac caaaaattcg
     3481 tcatcggcga catcgtaccg aacctggcga tcgggtaccc cttctgatgg ttcctggttt
     3541 cgggttccgg gtttcgagtt tcgggttgtt cgtggttagt tattggtgac tggtgattgg
     3601 atatttgtta ctagctattg gtggttgatt attaatcaca aatcacgaat atctaatcac
     3661 aagtcccaat cactgttcac tgttcactgt tcactgttta ctgtttactg ttcactgttc
     3721 actcccccac ccaattccac aataaatcct acctttaccg ccattcaaaa gcatgcacac
     3781 cactatgtca tctgcgaaaa tcgctgagtt gctgggagcc ggcgctgaga gcctgctgaa
     3841 ccaccagtcc aagaccattt ccaaagaaca ccttcacctt ccgggacccg atttcctcga
     3901 ccgcatcttc gcccctacca accgcagcaa ccaggtcatc cgcagtctgc aggccttgta
     3961 tggacacggt cgtctgaaag acaccggcta cgtttccatt ctgccggtgg accagggtat
     4021 cgaacattcg gccggtgcat ccttcgcgcc gaacccgatc tacttcgatc cggagaacat
     4081 cgtgaagctt gccatcgaag gcggctgcaa tgccgttgct tccacctatg gtgtgcttgc
     4141 cgccgtttcg cgcaagtacg cgcacaagat cccattcatc gtcaagatca accacaacga
     4201 attcctttcg tacccgaaca agttcgacca gatcatgttc ggcaccgtgc aggaagcctg
     4261 gaacatgggc gctgtcgccg taggcgctac gatttatttc ggttccgatg agtcggcccg
     4321 ccagatccag gaagtgagcg aagcattcga atacgcgcac gaactcggta tggcgaccat
     4381 tctctggtgt tacctgcgta accccggctt caagaaggac ggtaaagact atcataccgc
     4441 cgccgacctg accggtcagg ccaaccacct cggcgtaacg atacaggccg acatcattaa
     4501 gcagaaactg cccaccaaca acggcggtta tacggccgtc gcattcggca agacgcatcc
     4561 gaaagtgtac agcgaactca gctccgatca tccgatcgat ctttgccgct accaggtagc
     4621 caactgctac atgggccgca tgggcctcat caattccggc ggcgaatcca aaggcgcgag
     4681 cgacctttcc gaagccgtaa ccaccgcggt catcaacaag cgggcgggcg gacaaggact
     4741 gatctccggt cggaaggcct tccagaagcc gatgacggaa ggcgttgccc tgctcaacgc
     4801 cattcaggac gtttacctgt ccaaggatat caccatcgcc tgatcattct ccatgccgaa
     4861 cagccgttgt aaagc
//
LOCUS       DAOTON010000094         4871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_14348_length_4871_cov_3.824336, whole genome shotgun sequence.
ACCESSION   DAOTON010000094 DAOTON010000000
VERSION     DAOTON010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14348_length_4871_cov_3.824336"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /gene="nuoF"
                     /locus_tag="P5292_02900"
     CDS             complement(<1..1267)
                     /gene="nuoF"
                     /locus_tag="P5292_02900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRS38104.1"
                     /translation="MGKKLLLQNDRIPGIQTYEVYRKNGGYASVEKAIKTMTPEAVVE
                     EVKKSGLRGRGGAGFPTGMKWSFLAKPPGVPRYLVCNADESEPGTFKDRYLMERIPHL
                     LVEGMITSSFALGAHTSYIYIRGEYFYVARVLEQAIREAYANGWLGKNILGSGYDLDL
                     YVQVGAGAYICGEETALLESLEGKRGNPRIKPPFPAIAGLYGCPTVVNNVETIAAVVP
                     IVNDGGDAYAAIGIGKSTGTKLISASGHINKPGVYEIELGVPVEEFIYSEAYCGGIRN
                     GKKLKAVVAGGSSVPILPADLILKTANGEPRLMSYESLADGGFATGTMLGSGGFIVMD
                     EDTSIVKNLWNFTRFYHHESCGQCSPCREGTGWMEKILHRILDGHGDMSDIELLWDIQ
                     SKIEGKTICPLGDAAAWPVASAIRHFRKEF"
     gene            complement(1271..1807)
                     /gene="nuoE"
                     /locus_tag="P5292_02905"
     CDS             complement(1271..1807)
                     /gene="nuoE"
                     /locus_tag="P5292_02905"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRS38105.1"
                     /translation="MTQIKFSDESLNLVQELIARYPEGRQKSALIPVLHIAQAEFDGW
                     LSAPVMDYVASLLKLQPIEVYEVASFYSMFNLKPVGKCVIEVCRTSSCWLLGAEDVIR
                     HLEKKLNIKVGQTTADGMFTLKSVECLGSCGTAPMMQVGADYYENLNLQKVDQLVDRF
                     RADNKRKSYTDTPEPRNV"
     gene            complement(2079..3290)
                     /locus_tag="P5292_02910"
     CDS             complement(2079..3290)
                     /locus_tag="P5292_02910"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRS38106.1"
                     /translation="MDSIKLPLTEEPKEYSTLNLGPTHPATHGIFQNVLKMDGEIIID
                     SEPTIGYIHRAFEKLAEHKPFYQVTPLTDRLNYCSSPINNMGWHMTVEKLIKVEIPKR
                     VDYLRVIIMELARIADHLVCNSVIAVDTGAMTGFLYVFQWREFIYEIYEEICGARLTT
                     NIGRIGGLERPFNETVWRKIKHLLKELPAGLKEFESLVMRNRIFMDRTIGCGPITAEK
                     ALSYSFSGPNLRAAGVDYDVRVMNPYSSYQDFQFDIPVGVNGDTYDRFMVREEEMWQS
                     LSIIRQAMEKLEKLEDQTSFHADVPSFYLPPKEDVYNNMEALIYHFKIVMGETDVPVG
                     EVYHCVEGGNGELGFYLVSDGGRQPFRLHFRRPCFIYYQAYPEMIKGGFLSDAILTMS
                     SMNVIAGELDA"
     gene            complement(3302..3841)
                     /locus_tag="P5292_02915"
     CDS             complement(3302..3841)
                     /locus_tag="P5292_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002687749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRS38107.1"
                     /translation="MEKPTIDQVLAALREKFGEAVLSAHLEYDFQVLTLRKDMVLAIV
                     RFLYDDERFRFRYLTFATGIHIPNTEKPFGMVYQLHSLENNLRIRLKAFTTDHDIHFD
                     SLTPVFPAANWMERETFDFFGYKFHGHPNLKRILNVDDMDYHPLRKEYPVEDQTRYDK
                     DDTLFGRKPSQYDRKRLMS"
     gene            complement(3844..4401)
                     /locus_tag="P5292_02920"
     CDS             complement(3844..4401)
                     /locus_tag="P5292_02920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HRS38108.1"
                     /translation="MSDIQTSNIKIAKAPEGFEGPGFFATSLDKVVGLARKNSIWPLP
                     FATSCCGIEFMATMASHYDLSRFGSERLSFTPRQADLLMVMGTIAKKMGPVLRQVYEQ
                     MAEPRWVLSVGACASSGGIFDSYSVLQGIDHIIPVDIYVPGCPPRPEQILEGVMNIQK
                     LVHNESLRRRNSPEYKQLLASYGIE"
     gene            complement(4447..4818)
                     /locus_tag="P5292_02925"
     CDS             complement(4447..4818)
                     /locus_tag="P5292_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRS38109.1"
                     /translation="MNPQSQPADYFPILLQFIVALGFVVTTLVATHLLGPKRKSKVKL
                     DPFECGIESKGNARIPFSIKYFLVAILFVLFDVEVIFMYPWAVNFKTLGVTGILEMFL
                     FMGLLLTGFFYVMKKGAFKWE"
BASE COUNT         1062 a         1272 c         1371 g         1166 t
ORIGIN      
        1 cgaactcttt ccggaagtgt cggatggcac tggccacggg ccaggcggcc gcgtcgccaa
       61 gcgggcagat ggtcttgcct tcgatcttgc tctggatatc ccacagcaac tcgatatcgc
      121 tcatgtcgcc gtgtccgtcg aggatacggt gcaggatctt ctccatccag ccggtgcctt
      181 cacgacacgg actgcactga ccacaggatt catggtggta gaagcgggtg aagttccaga
      241 ggttcttgac gatgctggtg tcttcgtcca tcacgatgaa gccgccggag ccaagcatgg
      301 tgccggtggc gaaaccgcca tccgcgaggg attcgtacga catcaggcga ggttcgccgt
      361 tggccgtttt caggatgaga tcggcgggaa gaataggaac ggaagagccg ccggcgacta
      421 ccgccttcag ctttttaccg ttacggatac caccgcagta tgcttcggag taaatgaatt
      481 cttctaccgg tacgccgagt tcgatctcgt aaacgcccgg tttgttgata tggccgctgg
      541 cggagatcag cttggtgccg gtgctcttac cgataccgat agccgcatag gcatcgcccc
      601 cgtcgttcac gatcggtacg accgcggcaa tggtttctac gttgttcact acggtcggac
      661 aaccgtacag acccgcgata gctgggaacg gaggcttgat gcgcggattg ccacgcttgc
      721 cttcgagtga ttctaataat gctgtttctt cgccgcagat gtaagcgccg gcaccgacct
      781 gtacgtacag gtcgagatcg tagccggagc cgaggatatt ctttcccagc caaccgttgg
      841 cgtatgcctc ccggatggct tgttcaagga cacgggcgac atagaaatat tcgccgcgga
      901 tgtagatgta cgaggtgtgt gcgcccagcg caaaactgga cgtgatcatg ccctccacca
      961 agaggtgggg aatccgctcc atcaggtagc ggtctttgaa cgtgcctggc tcggactcgt
     1021 cggcgttgca aacgaggtaa cgcggtacgc ccggaggctt cgcgagaaaa ctccacttca
     1081 taccggtagg aaagcctgca ccgccgcggc cgcgcaagcc ggattttttc acctcctcga
     1141 cgaccgcctc cggcgtcatc gtcttgattg ctttctccac tgaagcgtaa ccgccgtttt
     1201 tacggtacac ctcgtaagtc tggatgcccg gaatccggtc gttttgcagg agcagtttct
     1261 ttcccatgga tcagacgtta cggggttcgg gagtgtcggt gtaggattta cgcttgttgt
     1321 cggcccggaa acggtccacg agttgatcga ctttctgcaa gttgaggttc tcgtaatagt
     1381 cggcgccgac ctgcatcatc ggagcggtgc cgcaggagcc gaggcattcc accgatttga
     1441 gggtgaacat gccgtcggca gtggtctgcc cgactttgat gttcagtttc ttttcgaggt
     1501 gacgaatcac gtcttccgca cccaacaacc agcacgaact cgtccggcag acttcaatca
     1561 cgcacttgcc aaccggtttg aggttgaaca tggaatagaa cgaggcgact tcgtaaacct
     1621 cgatcggctg cagcttgagt aaggaagcga cgtagtccat taccggcgcg ctcagccaac
     1681 cgtcaaattc ggcttgggca atgtgcaata ccggaatcag cgccgacttc tgccggccct
     1741 ccggataacg cgcgatcagc tcctgtacga gattcagcga ttcgtcggag aattttattt
     1801 gagtcatggg tgaattcgat ctgacttgta ctgattagga gcgaatagcg aatagtgaat
     1861 agcgaagagt gaattggggc ggattgaatt tcttttacac aggaaagatt agtcttctct
     1921 ctactcttgc cttacaagca ctttcaagct tttacttatc caagagctaa ttacgaatga
     1981 ccatttacta tttactgttc gctattcgct attcactatt tactgttcac tgttcactgt
     2041 tcactgttca ctgttcacta ttcactgttc actccccctc aggcatccaa ctcccccgca
     2101 atcacattca tcgaactcat ggtcaggatg gcgtcgctga ggaagcctcc tttgatcatt
     2161 tcgggatagg cctggtagta gatgaagcag ggtcggcgga agtgcaggcg aaaaggctgg
     2221 cggccaccgt cgctgacgag gtagaagccg agttcgccgt tgccgccttc gacgcaatgg
     2281 tagacttccc cgacgggaac gtccgtttcg cccatcacga tcttgaaatg atagatgagc
     2341 gcttccatgt tgttatacac gtcctccttc ggagggagat agaaggaggg tacatcggca
     2401 tggaagctgg tctggtcctc gagtttttcc aacttctcca ttgcctggcg gatgatcgac
     2461 aggctctgcc acatttcttc ctcgcgtacc atgaagcggt cataggtgtc gccgttcacc
     2521 ccgaccggaa tgtcgaattg gaaatcctga taagaagaat aaggattcat cacgcggacg
     2581 tcgtagtcga cgccggcagc gcgaaggttc gggccgctga agctgtagga taatgccttc
     2641 tccgccgtaa tcgggccgca gccgatggtg cggtccatga agatgcggtt acgcatcacc
     2701 aggctttcga attccttcag tccggcaggt agttctttca ggaggtgttt gatcttgcgc
     2761 caaaccgttt cattgaacgg tcgctccagg ccgccgatgc gaccgatgtt ggtcgtcagg
     2821 cgggcgccgc agatctcttc gtagatctcg taaatgaatt cgcgccactg gaaaacatac
     2881 aggaagccgg tcatggcgcc ggtatccacg gcgatcacgc tgttgcagac caggtgatcc
     2941 gcaatgcgcg ccagttccat gatgatgaca cgcaggtagt cgacgcgttt cgggatctct
     3001 actttgatca gtttctccac cgtcatgtgc caccccatgt tgttgatggg ggaggaacag
     3061 tagttgagac ggtcggtcag cggcgtgacc tgatagaacg gcttgtgctc ggcgagtttc
     3121 tcgaatgccc gatggatata accgatcgta ggctcggagt cgatgatgat ctcgccgtcc
     3181 attttcagga cgttctggaa aataccgtgc gtggccggat gggtgggacc caggttgagg
     3241 gtcgaatatt ctttgggttc ttccgtcagc ggaagtttga tcgaatccat gcgggtgcgg
     3301 ttcaggacat caaacgtttg cggtcatact gggatggctt gcgaccgaag agcgtgtcat
     3361 ccttgtcgta acgggtctgg tcttccaccg gatattcttt tcgaaggggg tgatagtcca
     3421 tgtcatccac gttcagaata cgcttcaggt tcggatgtcc gtggaatttg tatccgaaga
     3481 agtcgaaggt ctcacgctcc atccagttcg cggcggggaa taccggcgtc agcgaatcga
     3541 aatgaatgtc gtgatccgtc gtgaaggctt tgagccggat gcggaggttg ttttccagac
     3601 tgtgcaactg gtacaccatg ccgaacggct tttccgtatt gggaatatgg atgcccgttg
     3661 caaaggtgag ataacggaaa cggaagcgtt cgtcgtcgta caggaagcgc acgatggcaa
     3721 ggaccatatc cttccgcagg gtcagcacct ggaagtcgta ttcaagatgc gccgataaaa
     3781 cggcttcccc gaatttctcc cgtagggctg caagtacctg atcaatcgtt ggtttttcca
     3841 tggtcattcg atgccgtagc tggccaggag ttgtttgtat tcgggagaat tgcgacgacg
     3901 aagcgattcg ttgtgcacga gtttctgaat gttcatcacg ccttccagaa tttgttccgg
     3961 tcggggagga catcccggta cataaatatc gaccggaatg atgtggtcga tgccctgcag
     4021 cacggagtag ctgtcgaaaa taccgccgct ggaagcgcag gcgcctacac tcagcaccca
     4081 ccgcggttcc gccatctgtt cgtacacttg gcgcagcaca ggtcccatct tcttggcaat
     4141 cgtacccatg accatgagca ggtcggcttg tcgcggagta aaactcagtc gctcggaacc
     4201 gaaacgtgag agatcatagt gcgaggccat ggtggccatg aattcgatcc cacaacagga
     4261 cgtggcgaac ggcaaaggcc aaatggaatt cttacgcgcg agtcctacga ctttgtcaag
     4321 ggaagtcgcg aaaaaccccg ggccttcgaa gccttccggc gctttcgcga tcttgatgtt
     4381 ggaggtctgg atgtcggaca ttgacttggg attttgcagg aacgatttat tcagcttccg
     4441 ctgtcatcat tcccacttga atgcgccttt cttcatgaca taaaagaatc cggtcagcag
     4501 gaggcccatg aacaggaaca tttccaggat gccggttact cccagcgtct tgaagttcac
     4561 ggcccaggga tacatgaaaa tgacttccac gtcgaacaac acgaacagga tggcaaccag
     4621 gaagtatttg atcgagaatg gaattcgggc attccccttc gactcgattc cgcattcgaa
     4681 cggatccagc ttgaccttcg atttacgttt tggcccaagc aagtgcgtag ccaccagggt
     4741 ggtaaccacg aaacctaggg caacgatgaa ttgcagcagg atgggaaagt aatcggcagg
     4801 ttgactttga ggattcatgc tccggatttc aggcatttaa cagcgaaatc ggtcgcaaag
     4861 ttaggctttt t
//
LOCUS       DAOTON010000095         4833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_14478_length_4833_cov_2.730640, whole genome shotgun sequence.
ACCESSION   DAOTON010000095 DAOTON010000000
VERSION     DAOTON010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14478_length_4833_cov_2.730640"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="P5292_02930"
     CDS             <1..1011
                     /locus_tag="P5292_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38110.1"
                     /translation="SEPVRTSYGWHIIKMMERKPLASYEEMQNDLKGRVQKDSRSELS
                     KSSMINRVKSKYAFKENPKTRDEFNKVVDSTLTQGKWTADRASALKSVMFSLGNKSYT
                     QQDFATYVADHQSKRGGTIAPQVMVSNLYNEWVNESVLAYEESKLDSLYPDFRNLMQE
                     YRDGILLFELTDKKVWSKAVKDTAGLKSFYEANKAKYPWPDRLDATIYTCANADIAKQ
                     VHKLMKTVDDVDTLMSRINKTSQLNLQVKTGKFAKGDNDVIDKIEWKKGLSKDMPHNN
                     QVVFVDVHNVLPAGPKSLDEAKGLVTADYQSSLEKEWIEQLRSKYAVKINQDVVDSVA
                     NK"
     gene            1086..1961
                     /locus_tag="P5292_02935"
     CDS             1086..1961
                     /locus_tag="P5292_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRS38111.1"
                     /translation="MLSPKLRLILPLAALLMLQGTGCKYFSGRKSDNPGEGAIARAFD
                     RYLFPEDLEGVVPVGASHDDSAAATTAYVENWIRQQTVLHLAEEHLDDEQKEVEQKLE
                     DYRRSLLIHAYEAELVRQKLDTNVTEEEIEAYYQQNKKNFELRDNIIKVVYLKLPRKA
                     PKIGKVRDLFSSTRPRDREALADYCRQYAVNYFLDDSTWLLFDDLLKEIPIKTYDKEQ
                     FLQNNRNIEIEDSTNIYLVSIRGFMIKNSTSPLSFERNNIRTLIINERKLKLIGEMEQ
                     QAYEEARKNGKAEVY"
     gene            1980..3338
                     /locus_tag="P5292_02940"
     CDS             1980..3338
                     /locus_tag="P5292_02940"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRS38112.1"
                     /translation="MTCRRTGIAAWLILLTVFLHSTLSRAQTLIDQVVATVGNQIILR
                     SDIEKQYMQYLAQGGEAAEEARCGIFDQLILSKLMVNQAAIDSVDVPEAQVESELDRR
                     MRFYVRQIGSEQKLEEYFKTTIRQLKVELRDMIRDQLLVQTMQGKITKDVTATPNDVR
                     EYFNSIPPDSLPYIDAELEVAQIVRRPPVSDAERKAVRSRLEEFRKKILAGEDFAVYA
                     ALYSQDPGSAKKGGELGFFERGTMVPEFEATAFNLKPGEISPIIETKFGFHVLQLVER
                     RGDQINVRHILLVPKTGDDDLIKCVNTLDSIRQQINKGSITFEDAALKFSDDEDTRNN
                     GGLMINPETNTTRLSPDKIDRLLFFQVDTLPTGRISPPLMMNNADGKTAYRIVMVKTR
                     TKPHQANLRDDYQKIQEVALSEKQAKVLSDWVDKKRKSTYININPEFSSCQELQHWQT
                     GQ"
     gene            3373..4341
                     /locus_tag="P5292_02945"
     CDS             3373..4341
                     /locus_tag="P5292_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS38113.1"
                     /translation="MKQYSSDVEAVQDLVAKYRELRSEIGKVIIGQDEVVRDVIISIF
                     SRGHCLLVGVPGLAKTLLVNTIARSLGLTYNRIQFTPDLMPSDIIGTEVLDENRQFRF
                     IKGPLFANIILADEINRTPPKTQSALLESMQEYAVTAAGKRYPLDQPFFVLATQNPIE
                     QEGTYPLPEAQLDRFMFNVLLTYPAFEEEVTVVKNTTSNYSPELKPVLNGEEIRFFQE
                     LVRRIPIPDNVLQYAVTLAAKTRPNTPAATAQVNQYVSWGAGPRASQYLVIGAKCHAA
                     LNGKFSPDAEDVRAVAVPILRHRIVRNYRAEADGISVEAIIEGLLK"
     gene            complement(4367..>4833)
                     /locus_tag="P5292_02950"
     CDS             complement(4367..>4833)
                     /locus_tag="P5292_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38114.1"
                     /translation="REVAELERRKQRTDVAGQRFEQLSQQLEERMERDKSKREELRKL
                     AELGRKMQSFAEEWEKSKDKKQVIKKFVGNMTAEKKKKAAEHTPEKVAKRKAALLDRL
                     RKEIRVGSSVRMMKSRQVGIVEEIRKDVVFVSFGNLRASVSLENLELVTDPS"
BASE COUNT         1183 a         1362 c         1261 g         1027 t
ORIGIN      
        1 tccgaaccgg tccgtacttc ctatggctgg cacatcatca agatgatgga gcgcaaaccg
       61 ctcgcctcgt atgaggaaat gcagaacgac ctgaaagggc gtgtgcagaa agattcccgc
      121 tccgaactga gcaagtcgtc catgatcaac cgcgtcaaat ccaagtacgc tttcaaggag
      181 aatcctaaaa cgcgggatga attcaacaag gtggtcgaca gtaccctgac ccagggtaaa
      241 tggacggccg atcgcgcctc cgctttgaag tcggtgatgt tctccctggg caacaaatcc
      301 tatacgcaac aggatttcgc aacctacgtg gccgatcacc aaagcaagcg cggtggtacc
      361 atcgctccgc aggtcatggt cagcaatctc tacaatgaat gggtaaacga aagtgtcctg
      421 gcgtatgagg aatcgaaatt ggattcgctg tatcccgact tccgtaacct gatgcaggaa
      481 taccgcgatg gtatcctgct gttcgaattg accgacaaga aggtgtggtc aaaagccgtg
      541 aaggataccg cgggtcttaa gtcgttttat gaagcgaaca aggctaagta tccctggccg
      601 gaccggctcg acgctacgat ctatacctgt gccaatgccg atatcgccaa gcaggtgcac
      661 aagttgatga aaacggtcga tgatgtcgat acgcttatgt cacggatcaa caagacctcg
      721 caactcaatc tccaggtcaa gaccggaaaa ttcgccaaag gcgacaatga tgttatcgat
      781 aagatcgagt ggaagaaggg cttgtccaag gatatgccgc acaataacca ggtggtgttc
      841 gtggacgttc ataacgtcct gcccgcaggt ccgaagtccc tcgacgaggc taagggcctc
      901 gtaaccgccg attaccagag ctcgctggaa aaggaatgga tcgagcaatt gcgctcgaag
      961 tatgctgtca agatcaacca ggatgttgta gattccgtgg ccaacaagtg atccggccct
     1021 ccaatattac ccgcacgatg gcgttcccat tacaggagcg ccatcggcgt ttctgaatcc
     1081 acccgatgtt gtcaccaaag ctccgcctca tcctgccgct tgcagctctc ctgatgcttc
     1141 aggggaccgg atgcaagtac ttttcaggaa ggaagtctga caacccgggt gaaggggcca
     1201 tagcacgggc gttcgaccgc tacctctttc cggaagacct cgaaggggtc gtcccggtcg
     1261 gcgcctccca cgacgatagc gcggccgcga cgactgcgta cgtggagaac tggatccggc
     1321 aacagaccgt cttgcacctg gcggaagaac acctggatga cgaacagaag gaagtcgagc
     1381 agaaactgga ggattatcga cgttcgctgc tcattcatgc ctacgaagcg gaattggtcc
     1441 gccagaaact cgataccaac gtgaccgagg aagagatcga ggcatactac caacagaata
     1501 agaagaactt cgaactgcgc gacaacatca tcaaggttgt ttacctcaaa ctccccagga
     1561 aggcgccgaa aattggaaag gtgcgcgacc tgttctccag cactcgtccg cgcgaccggg
     1621 aagcgctggc cgactactgc cggcaatacg cagtcaacta cttcctggac gactcgacct
     1681 ggctcttgtt cgatgacctg ttgaaagaga ttccgatcaa aacctacgat aaggaacagt
     1741 tcctgcagaa taaccggaac attgagatcg aggactccac gaacatctat ctggtctcca
     1801 ttcgcggatt catgatcaag aacagtacct cgccgctcag cttcgagcga aacaacatcc
     1861 ggacgctgat catcaacgaa cggaaactca aattgatcgg agaaatggag cagcaggcct
     1921 acgaagaggc ccgtaagaat gggaaggccg aagtctatta aaacccgtat ttttgctcca
     1981 tgacctgtcg acgaaccggt attgccgcct ggctgatact cttgactgtt tttctgcatt
     2041 ccactttgag ccgggctcag accttgatcg accaggtggt ggccaccgtc ggcaatcaga
     2101 tcatcctgcg ttcggatatc gaaaagcaat acatgcaata cctcgcccag ggcggggaag
     2161 cggctgaaga agcccgctgc gggatctttg accaattgat cctttccaaa cttatggtga
     2221 accaggcggc cattgacagc gtcgatgtgc cggaagccca ggtggagagt gaactggacc
     2281 gccggatgcg tttctacgtg cggcagatcg gttccgaaca gaaactggaa gagtatttta
     2341 agacgaccat ccgtcagctc aaagtcgaat tgcgcgacat gattcgtgat caattgctgg
     2401 tgcagaccat gcagggaaaa atcaccaagg atgtgaccgc cacgccgaac gatgttcggg
     2461 agtactttaa ctccattcct cccgacagcc tgccgtatat cgatgctgaa ctggaagtgg
     2521 cgcagatcgt gcgccgtcct ccggtcagcg atgcggaacg aaaagcggtt cgttcccgac
     2581 tcgaagaatt ccggaagaag atactggccg gcgaagattt cgccgtgtat gccgcgcttt
     2641 actcacagga ccccggttca gcgaaaaagg gtggggaact cggcttcttc gagcgcggta
     2701 cgatggtgcc ggagttcgaa gcaacggcct ttaacctgaa acccggcgag ataagcccga
     2761 tcatcgagac caagttcggt tttcacgtcc tgcaactggt cgagcgcagg ggagaccaga
     2821 tcaatgttcg ccacattctc ctcgttccaa agaccggcga tgacgatctg atcaagtgtg
     2881 tcaataccct cgattcgatc cggcagcaga tcaacaaagg atcgattacc ttcgaagatg
     2941 cggccttgaa attctccgac gatgaagata cccgcaacaa cggcggtctc atgatcaatc
     3001 cggagaccaa cacgacccgc ctttctcccg ataagatcga tcgcctgctg ttcttccagg
     3061 tagacacgct tccgaccggc cgcatctcac cgccactcat gatgaacaat gcggatggta
     3121 agacggctta tcgtatcgtg atggtcaaga cccgtacgaa gccgcaccag gcgaatctcc
     3181 gggacgatta tcagaagatc caggaagtgg cgctgtcgga aaagcaggcg aaagtgctca
     3241 gcgactgggt ggataagaaa cgcaaatcca cctacatcaa catcaacccc gaattctcct
     3301 cctgccagga attgcagcac tggcagaccg gacaatgatc cccgccgcag ccaacgagac
     3361 ccctgacaac ttatgaagca gtattcaagc gatgtagaag ccgtacaaga cctggttgcc
     3421 aaataccggg aacttcggtc ggagatcggc aaagtgatca tcggtcaaga tgaagtcgta
     3481 cgcgatgtca tcatctccat cttcagccgc ggccactgtc tgctcgtcgg tgttccggga
     3541 cttgccaaga cgctgctggt aaacaccatc gcccgctcac tcggcctcac gtacaaccgc
     3601 atccagttca cacccgacct gatgccttcc gatatcattg gtacggaggt attggacgag
     3661 aaccgtcaat ttcgatttat caaaggcccg ttattcgcca acatcatcct cgccgacgag
     3721 atcaaccgta cgcctccaaa aacgcaaagc gcgttactcg agtccatgca ggagtacgcg
     3781 gtcacagccg ccggaaaacg ctaccccttg gatcagcctt tcttcgtact cgctacgcag
     3841 aacccgatcg aacaggaggg gacctatccg ctccccgaag cgcaactcga ccgattcatg
     3901 ttcaatgtgc tgttgacgta cccggcattc gaggaagagg tgaccgttgt gaagaatacg
     3961 accagtaact actcaccgga gttgaaaccc gtgctgaacg gtgaagagat tcgtttcttt
     4021 caggagctcg ttcggcggat accgatcccg gataacgtac tccagtacgc ggtgaccctg
     4081 gctgccaaaa cccgaccgaa tacaccggct gccacggctc aggtgaacca atacgtttcc
     4141 tggggtgccg gcccccgggc ttcccaatac ctggtgatag gagccaagtg ccatgcggcg
     4201 cttaacggca agttttcacc cgatgcggaa gatgtacgcg cggttgctgt cccgatcctg
     4261 cgccaccgga tcgttcggaa ctaccgggcg gaagccgatg gtatttccgt cgaagcgatc
     4321 atcgaaggcc tgctgaaatg attctggtgt agcgcgatcg gccctgctat gacgggtccg
     4381 tgaccagttc caggttctcc agactgacgc ttgcgcgaag gttgccaaag ctgacgaaga
     4441 ccacatcctt ccggatctct tctacgatcc ctacctgacg acttttcatc atccgtacgg
     4501 agctcccgac gcgtatttct ttacgcagtc gatcgagtag cgctgccttt cgtttggcga
     4561 ctttttccgg tgtgtgttcc gcggctttct ttttcttttc cgcggtcatg ttgcctacga
     4621 atttcttgat gacctgtttt ttgtccttcg atttttccca ttcttccgcg aacgactgca
     4681 tcttgcggcc aagttccgca agcttgcgga gttcttcgcg tttggatttg tcgcgctcca
     4741 tccgttcttc gagctgctga ctgagttgct caaatcgttg cccggcaaca tcggttcgtt
     4801 gcttgcgacg ttccaactcc gccacttccc gct
//
LOCUS       DAOTON010000096         4816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_14533_length_4816_cov_3.868305, whole genome shotgun sequence.
ACCESSION   DAOTON010000096 DAOTON010000000
VERSION     DAOTON010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14533_length_4816_cov_3.868305"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /gene="ric"
                     /locus_tag="P5292_02955"
     CDS             complement(<1..682)
                     /gene="ric"
                     /locus_tag="P5292_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HRS38115.1"
                     /translation="MLKEQTLAEIVTRHPMAAAVFERHKLDYCCRGKRTLQDACENDP
                     LLLNTVTTEVERVTRLEGGLSTYPEFDHLSLEELVQYIIGKHHHYVRSAMPLLDAHLR
                     KVAGKHGERHPELHRIQHLFAQVKDDMDQHMFKEERILFPRILEIERAAQSASMHWIP
                     DRHYITAPIHVMEAEHDEAGTALAEIHQLSSAYTPPDDACTTYRLVFEELKAFEQDLD
                     QHVHLENNL"
     gene            complement(774..1649)
                     /locus_tag="P5292_02960"
     CDS             complement(774..1649)
                     /locus_tag="P5292_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRS38116.1"
                     /translation="MTIQKARSIRKILVPTDFSENAANAARFAVELGRNMASSVVLYH
                     ACRVPVLASNEMMIAASNTDLERESKDRLRQFADRLQSETGYLNIDVRISSGFAVDEI
                     KELVEHEDFDLVVMGTKGATRLQSMLIGSNTADVISHCHCPVLAVPEDARFVPPARIV
                     FATNYADNDFQTLYMLADMFRSFNPEIMVVHVEENRHNRIEQRMLDWFEAQVVASIPY
                     DKFSFHLIHAEELEGALNDFLTSEKADMLAVATRRRGFFEKLTSKSLTRKLAFHSHLP
                     LLAFHARNVPSTPLF"
     gene            1807..2868
                     /locus_tag="P5292_02965"
     CDS             1807..2868
                     /locus_tag="P5292_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRS38117.1"
                     /translation="MNAVQKRILLTGILLALGIFGLGYYLGPDYHLEFLYSLVVLLTI
                     FVPGNRSTFDASVAMTGLILVGFFLSKDTVATNNRVLFTFFPVLIIWSFTFAIIKYKL
                     SQENLLKSTEHLNAMFRFATEGILISNSKGVIVMANPRACQLFGYDADGLNNRSIEDL
                     VPTRFRDHHAGFRKGYTQDPKNRPMGKGRDLFAKRSDGSEFPVEISLSTFVIKKEMFV
                     ISFLIDITERKLQDELIRQANEELEQRVEQRTAELAAANLSLATVNRTLHGEMATRAK
                     VEEALRDSERLFNTIAHNFPDGIICVLDRNLRVVLIDGNALQDLDIPREKLNGKSLQD
                     LQFLQLDDETKEKFRRVFQ"
     gene            2881..3726
                     /locus_tag="P5292_02970"
     CDS             2881..3726
                     /locus_tag="P5292_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRS38118.1"
                     /translation="MEFSYADQRFALNAVPLPDNKGFVKEILLVIHNVTEIRKAEQEM
                     KASLEKERSLNEMKSRFVSIASHEFRTPLSTILSSISLVDRYHQAGESEKARKHVERI
                     KTSVKNLTEILNDFLSLEKLEAGKVEIHPETINLVRFAEDLCEELQALAKPGQVIHYR
                     HTGEKEDVVSDKQLLRPICINLLNNAIKYSPENSRISFTTDTRNGIQLIVEDQGMGIP
                     LEDQQHLFDRFFRAGNVTAIQGTGLGLNIVRKYARLLGGDIRFTSEVQKGTTFTVNLP
                     ETATA"
     gene            3729..4787
                     /locus_tag="P5292_02975"
     CDS             3729..4787
                     /locus_tag="P5292_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS38119.1"
                     /translation="MKKILLIEDNADMRENTAEILELANYKVISAPNGRVGVEKASKE
                     NPDLIICDIMMPELDGYGVLYLLSKNPSTASIPFIFLTAKAEKADLRKGMSMGADDYL
                     TKPFEEMELLNAVEARLRKSDVFRKEFTKNIEGLSEFLAQARGLEELSRLSADRKVHH
                     FKKKEMIYMEGDEPNGIIFVVKGRIKTYKTNEDGKEFITGIHKEGDFLGYIDLIENTE
                     YRESAEAMDEAEVTIIPRQDFFSLLYSNRDVAAKFIKLLSNNLQETEDRLLNLAYNSV
                     RKRVADALITLQQRYHTAKDQNSGFSVSREDLASMVGTATESVIRTLSDFKEEKLIDI
                     KEKNIFILNPDRLSRMKN"
BASE COUNT         1234 a         1292 c         1240 g         1050 t
ORIGIN      
        1 agaggttgtt ctccaaatga acgtgttggt cgagatcctg ttcaaaggcc tttaactcct
       61 caaatacgag ccggtacgtc gtgcaggcat catccggagg cgtgtaagcc gaagaaagtt
      121 gatggatctc ggcgagcgcc gtccccgctt cgtcgtgctc ggcttccatc acatggatag
      181 gcgccgtaat ataatggcga tccggaatcc agtgcatact ggcactttgc gctgcgcgtt
      241 cgatctccag gatacgcgga aaaaggatac gctcttcctt gaacatatgc tgatccatgt
      301 cgtccttcac ctgtgcgaac aggtgctgaa tacggtgcag ctcgggatgt cgctccccgt
      361 gcttgccggc cacctttcgc aggtgagcat ccagtaaagg catcgctgaa cgtacatagt
      421 gatggtgttt tccgatgatg tattgcacca actcctccag actcaggtgg tcgaactcgg
      481 ggtatgtgga caggccgcct tccaagcggg taactcgttc cacttcggtg gtaaccgtgt
      541 tcaacaataa cggatcattc tcacaggcat cctgcaaggt gcgtttgccg cgacagcaat
      601 aatccagttt atgacgttcg aagacagcgg cggccatggg gtgccgtgtc acgatttcgg
      661 ccagcgtttg ttctttcagc atgacaatag cttatgcgcg taaagttcct ttataccaaa
      721 aggaaagtcg ctgacacaag tcatgcaatc cgctactcaa acacccctat tcgtcagaat
      781 aaaggtgtag acggcacatt ccgggcatgg aacgccagga gcggaagatg ggaatgaaaa
      841 gccagctttc gggtcaggct cttactggtg agtttttcga aaaagccgcg acgacgcgtc
      901 gccacagcca gcatatccgc tttctccgag gttaagaaat cattcagtgc gccttcgagc
      961 tcttccgcgt gtatcaggtg aaagctgaat ttatcgtagg gaatgctggc gactacctga
     1021 gcttcaaacc aatccagcat ccgctgttcg atccggttat gtcggttttc ttccacatgc
     1081 acgaccatga tctccggatt gaaggaacgg aacatgtccg caagcatata gagcgtctga
     1141 aaatcgttgt ccgcgtaatt cgtcgcgaag acgatacggg cgggcggtac aaaccgggca
     1201 tcttccggaa ccgccagtac cgggcaatgg cagtgactga tgacgtcagc agtattgctg
     1261 ccgatcagca tactctgcag acgtgtggca cctttggtac ccatgaccac caaatcgaaa
     1321 tcttcgtgct cgaccagttc cttgatctca tccactgcga aaccgctgct gatacgaaca
     1381 tcgatgttca gatatccggt ctcggactga agccggtcgg caaactggcg caaccgatcc
     1441 ttcgactcgc gttccagatc ggtattggaa gcggcgatca tcatctcgtt agaagcgagc
     1501 acgggtaccc ggcaagcatg gtacaacacc acactggagg ccatgttcct tcccaactca
     1561 acggcaaaac gcgcagcatt ggcggcattt tccgaaaaat cggtgggaac caaaatctta
     1621 cgaatggacc gggctttttg gatcgtcata gtatttcccc ttttctgatt tcaaaactat
     1681 cgaaagcagt gaccccgccc ctatgacctt tgtcattcat tatcattgac ctatggcagt
     1741 tttccggaaa ggacaagtgc actccatttt ctattttaaa tccattattt ttgaagaggg
     1801 atgcaaatga acgctgttca aaagaggata ctcctgaccg ggatcctatt ggcgctgggt
     1861 attttcggtt tggggtacta cctcgggcct gactatcatc tggagttcct gtactcgctg
     1921 gtggtcttgc tgaccatttt cgtacctggc aaccgctcca ccttcgatgc cagtgtggcg
     1981 atgacggggc tgatcctggt cggtttcttc ctgagcaagg acacggtggc gaccaacaac
     2041 cgggtgctct tcactttttt tccggtgttg atcatctggt ctttcacgtt cgccattatc
     2101 aaatacaagc tgtcgcagga aaatctgctc aaaagcaccg aacatctcaa tgccatgttc
     2161 cggtttgcta ccgaaggaat cctgatctcg aattccaaag gcgtcatcgt gatggcgaat
     2221 ccccgggcct gtcagttgtt cggatatgat gcggacgggc tgaacaaccg atccatcgag
     2281 gaccttgttc ctacccggtt tcgcgaccat catgccggat tccggaaagg ctatacgcag
     2341 gatcccaaga atcgtccgat gggaaagggc cgcgaccttt ttgctaaaag aagcgacggc
     2401 tccgagttcc cggtagagat aagtctcagc acattcgtga tcaagaaaga aatgttcgtc
     2461 atctccttcc tgatcgatat cacggagcga aagctacagg acgaactgat ccgacaggcg
     2521 aacgaagaac tggagcaacg ggtagagcag cgtaccgccg aacttgccgc cgcgaacctc
     2581 agcctggcca ccgtgaaccg taccctccac ggcgaaatgg ctacccgcgc gaaagtggaa
     2641 gaagccctgc gcgacagcga acgactattc aatacgatcg cccacaattt tccggacggg
     2701 atcatttgcg tattggaccg taacctgcgc gtggtactga tcgacgggaa cgcattgcag
     2761 gacctcgata ttccccgtga aaagctaaac ggcaagtcgt tacaggacct tcaattcctc
     2821 caattggatg atgaaacgaa ggaaaaattc aggcgggttt tccagtgaga aacggcttat
     2881 gtggaattca gctatgccga tcagcgcttc gcgctcaatg ctgttccgct ccccgacaac
     2941 aaaggttttg tcaaggagat cctgttggtc atccataacg tgaccgagat ccggaaagcg
     3001 gagcaggaaa tgaaagcgtc gctcgaaaag gagcgttccc tgaatgaaat gaagtcgcgt
     3061 ttcgtttcga tcgcgtcgca cgaattccgc acccccctga gtacgatcct ctcctccatt
     3121 tcactggtcg accgttacca ccaggccggt gaaagcgaga aagcccggaa gcacgtcgag
     3181 cgaatcaaga catccgtcaa gaacctgacg gagatcctga acgattttct ttcactggaa
     3241 aaactggaag ccggaaaggt cgaaattcat ccggagacta taaacctcgt gcgttttgcc
     3301 gaggatttgt gtgaggaact tcaggccctg gcaaaacccg gacaggtcat tcattaccgg
     3361 cacaccggcg aaaaggagga cgtggtatcc gacaagcaac tgctgcggcc gatctgcatc
     3421 aacctgctca acaacgcgat aaaatactcc ccggagaaca gcaggatttc gttcacaaca
     3481 gacacccgga acggcatcca actgatcgtg gaggaccaag gtatgggtat acctctggaa
     3541 gaccaacagc acctgttcga ccggttcttc agggccggaa acgtgacagc catacaggga
     3601 accggactag gcctgaacat cgtccgcaaa tacgcgcgtc tgctgggcgg cgacatccgg
     3661 ttcaccagtg aagtccaaaa aggcactacc tttaccgtca acctccctga aacggcaacc
     3721 gcctaactat gaagaagatc ctactgattg aagacaatgc cgacatgcgg gaaaataccg
     3781 cagaaatcct cgagctggcc aattacaaag tgatatcggc tccgaatggc cgtgtaggcg
     3841 tggagaaagc ctccaaagaa aatccggacc tgatcatttg cgacatcatg atgccggagt
     3901 tggatgggta tggcgtattg tacctgcttt ccaaaaatcc gtcgaccgcc agcattccct
     3961 tcatctttct tacggctaaa gccgagaaag ccgacctgag aaaaggcatg agcatgggcg
     4021 ccgacgatta cctcaccaag cccttcgaag agatggaact gttgaacgcg gtcgaagcac
     4081 gcctgcggaa gagtgacgta ttcaggaagg aattcacgaa gaacatcgag gggctcagcg
     4141 aattcctggc acaagcccgc ggcctcgaag agttaagcag gctttcggcc gaccgcaaag
     4201 tccaccactt caaaaagaag gaaatgatct acatggaagg cgacgagccc aacgggatca
     4261 ttttcgtcgt gaaaggacgt atcaagacct acaagaccaa cgaagacggc aaggaattca
     4321 ttaccggcat ccataaggaa ggcgatttcc tgggttacat cgatctgatc gagaacaccg
     4381 agtaccgcga atcagccgaa gcgatggacg aagcggaagt gacgatcatc ccccgacagg
     4441 atttcttctc gttgctctac tcgaaccgcg atgtggcggc caagttcatc aaattgcttt
     4501 ccaacaacct gcaggaaacg gaagaccggt tgctcaacct cgcgtataat tccgtacgga
     4561 aacgggtagc cgacgcgttg atcacgctgc agcagcgcta ccataccgcc aaggatcaga
     4621 actccgggtt ttccgtttcg cgcgaagatc tcgccagtat ggtcggaacg gccaccgaat
     4681 cggtcatccg taccttgtct gacttcaagg aagaaaagct gatcgacatc aaggagaaga
     4741 atatcttcat cctgaatccg gaccggcttt cacgcatgaa gaactgaacg gctgttagcc
     4801 acgaagcgcg tgaacg
//
LOCUS       DAOTON010000097         4797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_14596_length_4797_cov_3.676930, whole genome shotgun sequence.
ACCESSION   DAOTON010000097 DAOTON010000000
VERSION     DAOTON010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14596_length_4797_cov_3.676930"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4797)
                     /locus_tag="P5292_02980"
     CDS             complement(<1..>4797)
                     /locus_tag="P5292_02980"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRS38120.1"
                     /translation="ATFLDEYQESDDWIELFNSGSSPVDLCGYAISDDSLDLRKFVFP
                     SYSLGPQDHLLLVAADPVKTEITATWQTAVRENDTWRYRANTSAPPDTNWRNTSFNDG
                     SWNSGTGGIGYGDGDDGTTISTTISAYSRRTFSIADTAKILDAVFHADYDDAFVAYLN
                     GIEIARANIGVPGIRPAWNAVSASSHEAVMYQGQLPDSFHVDAATLKAALLPGNNVLA
                     IEVHNLNTTNTDLSGRFWLSFLCNPGAGSFYSLPSWFRAPAKMYFHTGFKLSRTGETV
                     WLSDPAGNVLDAKATGSLEADHAVARIPDGGSWCLTDQPTPDASNNASSCGSGYAGIP
                     VFSKAAGFYASAQSLTITTTYPGGQVRYTLDGSDVTSSSPLASGPITLNDTAITVRAR
                     VFASGVLPGTTITNSYFIGVNSRLPVYSLTTDPDNLWDYNTGIYVEGPNADPNTPHWG
                     ANYWMDWEKPVTVEFFDRARQRAFRFNGGLKITGGWSRSAPQKSFEINLGDRYGLSSL
                     DYPMESLKPWMTGWDNFILHMTGNDRSNARMRDPLMQRLLRTTFNDYVAYEPCLLFLN
                     GGNWGVYYTRENDDHHWVKLNYGYDDDEIDLLKESYFASGIEVKRGSTDAFWKLYNYA
                     TTVSPSDPAYYDSVASMMDLQNMADYFIAETYYPNDDWMGGGNNNLKLWRPRKEGGKF
                     RYLIYDLDFGLGLYGSVTNNMLSVARNPSPQNYNSDIFDALTQNTVFRRYFINRYADL
                     INTIFLPSNVQQVAYMYRDTISTDMAYQFAWGSNNSDWLSNIQSMLTFASQRPSNVRG
                     QIQSEFGLSGQVTLTLQVQPAGAGRIEISTITPTSYPWSGVYFNGNPVTITAIPNPGY
                     SFDHWRSNTTISSNNPNRSVTYNFTSSDAITAYFNGNAATAQVEFSEINFNSDSAWNT
                     GDWIELRNPAAFDLDISGWKFRDDADNHTYTLPTGTVLPAGGYLVLASDLDKFASINP
                     AVTNVIGDFGFDFNNGGEELRLFNRDDQLFGSVFYDDFAPWPVTADGAGFTLERVAGS
                     ADPNDPASWFAGCYGGSPGRAYGAPTIGLTAAGTTQLCQGATVTLTASPANATTYQWQ
                     RDGVDIPGATAVDYVASVTGVYRARVNAGGCTGLSDTLRVTVSAISSLTSTTGASRCG
                     DGSVQLQAVSTSPIEWYDAASGGTLVGTGTSFTTPVLQSTTTYYAQAAGNCPGPLVAV
                     TAVVNAVTADPVIQDGSRCGDGSLTLTATDTAAVRWYATASGGSVLASGYTFVTPALT
                     TTTTYYAEAGTLCPSNRVPAVATILPLTADPVTQSDSRCGPGSVTLTATDTAQIRWYD
                     APVGGTLLATGSSFTTPILTATTTYYAEAGDVCPSQRIPADAIIISGAALPATQDATR
                     CGPGTLLLTATPGDTATLYWYDAAVGGTLLSTGNTYTTPVLNTSTTYYVQAGISCPSA
                     RISVDAIITSNPVDPQVTGAQRCGSGTLTLAAVSSDPVSWYDAPGGNLLGTGLSFTTP
                     SISVDITYYAQSGSGSCLSNFIPVLAQINLVSVDPVVTGGSVCGFGQVLLTASAADPM
                     SWAATPGGAVVETGGTYLTPIIAVNTTYYVQAGTLCPSNWVAVTATV"
BASE COUNT         1015 a         1312 c         1503 g          967 t
ORIGIN      
        1 caccgtggca gttaccgcca cccagttgct tggacaaagt gtcccggcct gcacatagta
       61 cgtggtgttc accgcgatga tgggcgtgag gtaggttccg ccggtttcga ccacggctcc
      121 tcccggtgtc gccgcccaac tcatcggatc agcggcgctg gcggtcagca gcacctggcc
      181 gaatccgcaa accgaaccgc cggtgacgac cggatcaaca ctgaccagat tgatctgtgc
      241 caagaccggt atgaagttgc tcaggcaact gccgctgccg gactgcgcgt agtacgtgat
      301 atcgacactg atcgaaggcg tggtaaagct caagcctgtt cccaacaggt tgccgccggg
      361 ggcgtcgtac caggagaccg gatcgctgct gacggcagcg agtgtcagcg ttccgctgcc
      421 gcatcgttgc gcgccagtta cctgcggatc gaccgggttc gaggtgatga tcgcgtcaac
      481 ggagatgcgc gccgacggac aacttattcc ggcctgaaca tagtacgtcg tggaggtgtt
      541 gaggaccggc gtcgtgtagg tgttaccggt tgaaagcaat gtcccaccaa cagcggcatc
      601 gtaccaatac agtgtcgcgg tatcgcccgg tgtggccgtc agcaagagcg tacccggacc
      661 acatcgggtg gcatcctgcg ttgcaggaag agcagcgccg ctaataatga tcgcatcggc
      721 aggaatccgc tggctcgggc aaacatcgcc ggcctccgcg tagtaggtgg tcgttgcggt
      781 cagtatcggg gtagtgaagc tactaccggt agccagcaag gtgccgccga ccggcgcatc
      841 gtaccagcgg atctgcgcgg tatcggttgc cgtcagcgtt accgagccgg gtccgcagcg
      901 gctgtccgac tgtgtgaccg gatcggcggt aagcggaagt atcgttgcaa ccgccggcac
      961 gcggttactc ggacaaagcg ttcctgcttc cgcgtaatag gtcgtcgtgg tggtcagggc
     1021 gggcgtgacg aatgtgtagc ccgaagccag tacactgcca ccacttgcgg ttgcatacca
     1081 acgcacagcg gccgtatcgg ttgcagtaag cgtcaggctg ccatcaccgc agcgactacc
     1141 gtcttgtatc accggatccg cggtaacggc attcaccacc gcggtcaccg ctaccaacgg
     1201 gccgggacag ttaccagcgg cctgggcata gtaggtcgtc gttgactgaa gtaccggtgt
     1261 ggtaaagctc gtgcccgttc ccaccaaggt cccgccgctg gcggcatcgt accattcgat
     1321 cgggctggtg gacacagcct gcaattgcac actgccgtca ccgcaacggg aagcgccggt
     1381 agttgacgtg agtgaactga tcgccgaaac ggttacccgc agcgtgtcac tcagtccggt
     1441 acatccgccc gcgttcaccc gtgcacgata gactccggta acggaggcaa cgtaatcgac
     1501 cgcggtcgca ccgggaatat cgacaccgtc gcgctgccac tgatacgtcg tggcgttggc
     1561 aggtgaagcc gtcagcgtga cggttgctcc ctgacaaagc tgtgtcgtgc cagcagcggt
     1621 gagtccgatg gttggcgcac catacgcgcg tccaggagaa ccgccataac agccggcgaa
     1681 ccaactggcc ggatcattcg gatctgcaga cccggcaaca cgctcaagtg tgaagccggc
     1741 tccatcggcg gtaaccggcc agggcgcgaa gtcgtcatag aagaccgagc cgaacaattg
     1801 gtcatcgcgg ttaaagagcc ggagctcttc gcctccatta ttgaaatcaa atccgaagtc
     1861 gccgatcaca ttggttactg ccggattgat cgaagcgaac ttgtcaagat ccgaggccag
     1921 gaccaggtaa ccgcccgccg ggagtacggt gccggtcgga agggtgtagg tatgattatc
     1981 ggcatcgtca cggaatttcc aaccggatat atcaagatcg aaagcagcag ggttgcgcaa
     2041 ctcgatccag tcgccggtgt tccaggcgga atcgctgttg aaattgatct cgctgaattc
     2101 gacctgcgca gtggccgcgt tgccgttgaa gtaggccgtg atcgcgtcgc tggaggtgaa
     2161 attataggtg accgaacggt tcgggttgtt cgagctgatg gtcgtgttag aacgccagtg
     2221 atcgaacgaa taaccggggt tgggaatggc ggtgatcgtc accggattgc cattgaagta
     2281 aacgccggac cagggatagg atgtcggggt gatcgtggag atctcgatac gtccggcacc
     2341 ggccggttgg acctggagcg tcagcgtgac ctgaccggag agtccgaact cactttgaat
     2401 ttgtccgcgc acgttcgacg gtcgctgact agcaaaggtc agcatgcttt gaatgttgct
     2461 gagccagtcg gagttattgc tgccccaggc gaattggtac gccatgtcgg tcgagatcgt
     2521 atcccgatac atatacgcca cctgctgcac gttcgacggc aggaagatgg tgttgatcag
     2581 gtccgcgtaa cggttgatga agtagcgacg gaagaccgtg ttctgcgtca gcgcgtcgaa
     2641 gatgtcggag ttataattct gcgggctggg attgcgggcc acggacaaca tgttattcgt
     2701 gaccgaaccg tacaagccca aaccgaagtc gaggtcgtag atgaggtagc ggaatttgcc
     2761 tccttccttc cggggtcgcc agagcttcag gttgttgttg cctcctccca tccagtcgtc
     2821 gttcggatag taggtttcgg cgatgaagta atcggccatg ttctgaaggt ccatcatgga
     2881 cgcgacggaa tcgtagtagg caggatcgga gggcgaaacc gtcgtagcat agttgtacaa
     2941 cttccaaaag gcatcggtgc ttccacgctt gacctcgatg ccggaagcga aataactttc
     3001 cttcaggagg tcgatctcat cgtcgtcgta gccgtagttg agtttgaccc agtgatggtc
     3061 gtcgttctcc cgggtatagt acacgcccca gttgccgccg ttgaggaaca ggagacaagg
     3121 ctcataggcc acataatcgt tgaacgtcgt tcggagtagt cgttgcatga ggggatcccg
     3181 catgcgcgca ttcgaccgat cattgccggt catgtgcagg atgaaattat cccatccggt
     3241 catccagggt ttcaacgact ccatcggata atcgaggctc gaaaggccgt agcggtcgcc
     3301 gaggttgatc tcaaagctct tttggggagc ggaacgtgac cagccaccgg tgatcttcag
     3361 gccgccgttg aaacgaaatg cccgctggcg cgcgcggtcg aagaactcga ccgttaccgg
     3421 tttctcccaa tccatccaat agttggcgcc ccagtgcgga gtgttcggat cggcgttggg
     3481 tccttcgacg tatatgccag tgttgtaatc ccagaggttg tcgggatcgg tggtcagtga
     3541 atacaccggc aagcgactgt tcacaccgat gaaataagag ttcgtaatgg tagtacccgg
     3601 caatacgccg gaggcgaaca cccgggcgcg tacggtgatg gcggtatcgt tcaaggtgat
     3661 cggaccggaa gcaagcggag atgaggatgt cacatcactg ccgtcgagcg tatagcgtac
     3721 ctgtccgccc ggataggtgg tggtgatcgt cagcgattgc gcggatgcat agaagcccgc
     3781 ggccttcgag aagacgggaa tgccggcata cccgcttccg caggacgacg cgttgttcga
     3841 agcatcgggt gtcggttggt ccgtgaggca ccaggagccg ccgtcgggaa tgcgggcaac
     3901 ggcatggtcc gcttcgagac ttccggtggc tttcgcgtca aggacgtttc ccgcaggatc
     3961 ggaaagccag accgtctcac cggtgcgact cagtttaaaa cccgtatgga aatacatctt
     4021 cgccggagcg cggaaccagg aaggaagact gtagaaggaa cccgcgccgg gattgcacag
     4081 gaagctcaac cagaaacgac cgctgaggtc ggtgttggtg gtgttcaaat tatggacctc
     4141 gatcgcgagg acattattcc cgggcaagag tgcagccttc agtgtcgctg cgtccacatg
     4201 aaaagaatcg ggtaactgtc cctggtacat caccgcttca tgcgagctgg ccgagacggc
     4261 attccaagcg ggacgaattc cgggtacacc gatgttggcg cgggcgatct cgataccatt
     4321 gaggtaggcg acgaaagcgt cgtcgtaatc ggcatgaaat accgcgtcga ggatcttcgc
     4381 tgtatcggca atggagaatg tacggcgaga ataggccgat atcgtggtgg agatggtcgt
     4441 accatcatcc ccatcgccgt agccgatgcc gccggtaccg gaattccagg aaccgtcgtt
     4501 gaaggacgtg ttgcgccagt tcgtatcggg tggcgcggaa gtgttggcgc gataacgcca
     4561 ggtatcgttc tcccgcacgg cggtttgcca ggtggcggtg atctccgttt tcaccggatc
     4621 ggcagccacc agcagcaggt ggtcttgcgg tccgagcgaa tacgatggaa agacgaactt
     4681 ccgcaagtcg agtgaatcgt ccgagatcgc gtaaccgcac aggtcaaccg gagaacttcc
     4741 gctgttgaac aattcgatcc aatcatccga ctcctggtac tcgtcgagga aggtcgc
//
LOCUS       DAOTON010000098         4790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_14623_length_4790_cov_3.309398, whole genome shotgun sequence.
ACCESSION   DAOTON010000098 DAOTON010000000
VERSION     DAOTON010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14623_length_4790_cov_3.309398"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..792)
                     /locus_tag="P5292_02985"
     CDS             complement(40..792)
                     /locus_tag="P5292_02985"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRS38121.1"
                     /translation="MSAGKNIYFASDFHLGIPDRARSLERERRIVRWLEQIRPDTREL
                     YLMGDVFDFWFEYKTVVPRGYVRLLAAIAAFTDAGIPVHYFTGNHDMWTFGYLEQELG
                     VRLYRKPIEAEYDGKKFYIGHGDGLGPGDHGYKFIKKVFASPLCQWLFARLHPNFGIG
                     VADYFSRKSRVATGTAEDRYLGDEQEWLVQYCKDLQQNKRYDYMIFGHRHLPLDMIVN
                     GKGRYINLGDWIRYNTYAVFDGADLQLKKFEG"
     gene            complement(792..3797)
                     /locus_tag="P5292_02990"
     CDS             complement(792..3797)
                     /locus_tag="P5292_02990"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRS38122.1"
                     /translation="MNRLHHTLLCFFGLACLAASATRPYFQQALTYRIQVKLDDREHM
                     LRGQVAIDYTNRSDSSLDELYLHVWPNAYREDRSGLARELYGNQDPVFALTRPEERGF
                     MDSLDFRCDGQDVQWTYFSGAADIIRIKLQQPLAPGTSMTLATPFRVKLPDAAISRLG
                     HADGRYYLTQWYPKPAVYDRNGWNALTYQSWGEFYGEYARYEVELTLPESYWVAATGV
                     LKDQAEHTRRLLADSVTRAGGVVANAGLRPEATSAGTTLHFEADSVHDFALFAAKDYQ
                     VLADRAPGKPEIWSFYLQQHEQNWKLSNAVLRQALDSLTNWIGPYPYAQLSVVDVDDI
                     AGRDMEYPTIVAIGACSANQFLQTLYHEAAHQWFYGMLGSNERRHPWMDEGWTTYLEN
                     RLYLNTQRLVSSPKESKFSQWLRLSDEPEDFLPHLRLHYLKGASRNSDPLTGAAALFY
                     DESDYYTAAYSKPTLGIVWLEDVLGRDVFDLAMRNYYREWAFRHPQPEDLQTAFERVS
                     GKKLDWYFDDWTFTNAKADFALEVKTGKGGISRVTVMNKGEVNGPVSLAAYRDGQLLE
                     KRQISTATFNHAFDWKTDSADSFVLDPENRVPEVNRRNNFSRAQGLLRKTEPIRFQFF
                     IGHEVDRRTTIGLFPAVGWNTNNGLLLGGGLHNLNFEEKPFEALAMPLYGTEDKELNF
                     GGFVRYHFYPKTGNLRRIRLQSGWSRYAYATDSYQDTSGRVLADGGLHYLKADHSLQL
                     KFMHGSREKVESQLRFHAIQIRKDLPYAYYYSKTTRDDLFLTGSYERVTAFPDASHLT
                     VEAQWHPDMYKVTLTEQLLLPYRNPKKGLRLRLFGGYVSINNTSVAGQDFRLRLSGFE
                     GGDDYLFRDVFPGRNEPDGVFSHQFVLTEGGFTTPVSYFGKSSGWMLTARASTTLPGK
                     LPFRLYLAGGSFEDINDFRPEFGKVSFEGGVELPILGDIFVVYLPAVYSKDIRDALDR
                     LELSTAETIRFELRLRELNPIELARKYLQ"
     gene            complement(3838..>4790)
                     /locus_tag="P5292_02995"
     CDS             complement(3838..>4790)
                     /locus_tag="P5292_02995"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRS38123.1"
                     /translation="DSVTTDADGKFSFRNPVTELDYYILRANPTNLLFLILKGGETVE
                     INGDAQRLDETYSVKGSEDSESIRKLREYERKLTDSLNSIYAKARNENPLGKDSVGLK
                     LQAAYSRSMEHFAQDFIRQHNNSIASLSATKYLNQQQSLPLMSELEENLRKAYPDNKY
                     ARDFSALVQDLRKLPPGSLAPEIKLPGIDGQALALSSLRGKVVLVDFWASWCQPCRME
                     NPNLVRIYKKYRGDKFEILGVSLDRDAAAWKAAVVKDSLSWPQISELKMWESGFVKDY
                     NIDAIPFSVLLDANGKIIAKGLRGEDLELKIREQLGKTSS"
BASE COUNT         1047 a         1297 c         1295 g         1151 t
ORIGIN      
        1 ttcgactgaa atgacgttcg atgcagttgt ggccaggact cagccttcga actttttcaa
       61 ttgcagatcc gctccgtcga ataccgcata ggtgttgtac cggatccaat ctcccaggtt
      121 gatgtaccgg cctttgccgt tgacaatcat atcgagtggg aggtgtcgat gaccgaagat
      181 catgtagtcg tatcgcttgt tttgctggag gtccttgcag tattgaacga gccattcctg
      241 ctcgtctccg aggtagcggt cctcggcggt accggtagcg acgcggctct tgcgtgagaa
      301 ataatcggcc acgccgatcc cgaaattcgg atgcaggcgc gcgaacagcc actgacaaag
      361 cggactggca aatactttct tgatgaattt gtatccgtgg tcacccggtc ccagaccgtc
      421 tccgtgtccg atgtagaact ttttaccgtc gtattcggct tcgatcggtt tgcggtaaag
      481 ccggacgcca agttcttgtt ccaaatagcc gaacgtccac atgtcgtgat tgccggtgaa
      541 gtagtgcacc ggaataccgg catccgtgaa ggcggcgatg gctgccaaca agcgcacgta
      601 tcctcgtggt actacggtct tgtactcgaa ccagaaatca aacacatcgc ccatgagata
      661 cagttcccgg gtgtccggac gaatctgctc gagccagcgc acgatgcgcc gttcacgttc
      721 caggctgcgg gcccggtcgg gaatgccgag gtgaaaatcg gacgcgaaat aaatgttctt
      781 accggcggac atcactggag gtattttcgg gcaagttcaa tcgggttaag ttcgcgcagt
      841 cgcaattcga agcggatcgt ctcagcggtg gagagctcta aacggtcaag agcgtcgcga
      901 atgtctttgc tatagactgc cggcaaatag acgacaaaaa tatcgcccag gatcggtaat
      961 tcaacaccac cttcaaaact gactttgccg aattccggtc ggaaatcgtt gatgtcttcg
     1021 aaactcccgc cggccaggta cagccggaag ggcaatttac cgggaagtgt cgtgctggct
     1081 ctggcggtta gcatccagcc ggaggacttt ccgaaataac tcaccggtgt cgtaaacccg
     1141 ccttcggtga gtacgaactg atgactgaaa acgccgtctg gttcattccg acccgggaag
     1201 acgtcgcgaa acagataatc gtcgccgccc tcgaaaccac tgagccgtag tcggaaatcc
     1261 tgccccgcaa ccgaggtgtt gttgatcgaa acgtagcctc cgaacaacct taaccgaagt
     1321 cctttcttcg gattccggta gggaagcagc agttgctcgg tcagcgtgac tttgtacata
     1381 tccggatgcc actgtgcctc tacggtgaga tgactggcgt ccggaaaggc tgttacccgt
     1441 tcgtaggaac ccgtcaggaa aagatcgtct cgcgtcgttt tggaatagta gtaggcataa
     1501 ggcaggtctt ttcgtatttg aatggcgtgg aaccggagct gagattccac cttttcccgg
     1561 ctcccatgca tgaacttcaa ttgcagggag tgatccgctt tcaaatagtg caatccgcca
     1621 tcggctaata cacgaccact ggtgtcctgg taactgtccg tcgcgtaggc atatcggctc
     1681 cagccggatt gtaaacggat ccggcgcagg ttgccggttt taggatagaa atgatagcgt
     1741 acgaatccac cgaaattcaa ttccttgtct tccgttccgt agagtggcat cgccagcgct
     1801 tcgaatggtt tctcttcaaa gttcaggttg tgcaagccgc ctccgagcag caaaccgtta
     1861 ttggtgttcc agcccactgc cggaaacagg ccgatcgtcg tgcgacggtc cacttcgtgc
     1921 ccgatgaaga actggaaccg gatgggttcg gtttttcgga gtaagccctg cgctcgactg
     1981 aagttgttac gccggttcac ttccggtaca cggttttcag gatcaagaac gaaagaatca
     2041 gcgctatccg ttttccagtc aaatgcatgg ttgaacgttg cagtactgat ctgtcgcttt
     2101 tccaataatt gaccgtcccg atatgcggca agtgaaacag ggccgttgac ttctcctttg
     2161 ttcattaccg taacgcgtga tataccgcct tttccggttt ttacttccaa cgcaaaatca
     2221 gcctttgcat tggtgaacgt ccaatcgtcg aaataccaat ccagtttttt tccggaaacg
     2281 cgttcgaaag cggtttgcag gtcctcgggc tgtggatgcc ggaacgccca ttcccggtag
     2341 taattgcgca tcgcaaggtc gaagacatcc cggcccaaga cgtcttccaa ccagacaatg
     2401 cccaaggtcg gttttgaata ggctgccgta tagtagtcgc tttcatcata gaagagcgct
     2461 gctgctccgg taagtggatc ggagttcctg cttgcgcctt tcagataatg aagacgaagg
     2521 tgcggcagga aatcctcagg ttcgtcgctc aatcgcagcc actgtgagaa cttcgactct
     2581 ttgggcgagc tgaccaatcg ctgtgtattc aaatacaatc ggttctccag gtaagtggtc
     2641 caaccttcat ccatccaggg atgccgacgt tcgttgctgc ccagcattcc atagaaccat
     2701 tggtgtgcgg cttcatggta aagcgtctgc aggaattgat tggcgctgca tgctccgata
     2761 gccacgatgg tcgggtactc catgtcccgc cccgcgatgt catccacatc aaccacgctc
     2821 agttgtgcat acgggtacgg accgatccaa tttgtcagac tgtccagtgc ctggcgcagt
     2881 acggcgttgg atagtttcca gttttgttcg tgctgctgca aatagaaact ccagatttct
     2941 ggtttccccg gagcccggtc cgcaagtacc tgatagtcct tggccgcgaa aagcgcgaaa
     3001 tcgtgtacac tgtcggcttc gaagtgcaag gtcgtccccg cgctggttgc ttccggccga
     3061 agtccggcat tcgctacaac cccaccggca cgggtaacgg agtctgccag caaccgtcgc
     3121 gtatgttccg cctgatcctt gagtacgccg gtagcggcga cccagtaact ctccggtaat
     3181 gtcagctcga cttcataccg agcgtattct ccataaaatt ctccccagct ctgatacgtc
     3241 agggcgttcc agccatttcg gtcgtagaca gccggcttgg gataccactg cgtcaggtaa
     3301 taacgaccat cggcatgtcc cagtctcgaa atggcggcgt cgggcagttt aacccggaag
     3361 ggtgtggcaa gtgtcatgct cgtacccggc gccagcggtt gctgtaactt gattcgaatg
     3421 atgtcggctg ctcctgaaaa gtaggtccat tgtacatcct gaccatcaca ccggaagtcc
     3481 agcgagtcca tgaaaccgcg ctcttccggt cgggtcaatg cgaatacggg atcctggttt
     3541 ccgtaaagtt cccgcgccag cccggaacgg tcctcccggt aagcgttcgg ccaaacatgt
     3601 agatacaatt cgtccagtga gctgtcggag cggttggtgt aatcgatcgc aacctgcccg
     3661 cgcagcatgt gctcccggtc atccagttta acctgaatgc ggtaggtaag cgcctgctgg
     3721 aaatagggac gtgtcgctga agcggcaagg catgcaagcc cgaaaaaaca gagtaaggtg
     3781 tgatgtaatc gattcacgcg ggaaatgtag taagccggac aggatgagcg tgcggcttta
     3841 cgaagaagtt ttccctaact gctcccgtat tttgagttca aggtcctcgc cccgtagtcc
     3901 cttggcgatg atcttgccat tggcgtccag caaaacactg aatggtatcg cgtcaatgtt
     3961 gtaatccttc acgaagccgc tctcccacat cttcaattca ctgatctgtg gccaactcag
     4021 gctgtctttc acgacggccg ctttccaggc tgcggcatcc cggtccaggg atacgccaag
     4081 gatctcaaac ttgtcgcccc ggtacttttt ataaatgcgc accaggttgg ggttttccat
     4141 cctgcacggc tggcaccagc ttgcccagaa atcaaccaac accactttcc cgcgaaggga
     4201 agaaagtgca agggcttgcc cgtcgatacc gggaagtttg atctccggcg ccaggctgcc
     4261 gggcgggagt ttgcgaagat cctgtacgag ggcggaaaaa tcgcgggcgt acttgttgtc
     4321 cggataggcc ttgcgtaaat tctcttccag ttcgctcatc aggggcaggg attgctgctg
     4381 gttgaggtat ttcgttgccg atagcgaggc gatcgaattg ttgtgctgcc ggatgaaatc
     4441 ctgcgcgaaa tgctccatgc tgcgggaata ggcggcctgc aatttcaagc ccacggaatc
     4501 ttttccaaga ggattttcat tgcgtgcctt cgcgtagatc gaattgagtg agtcggtgag
     4561 cttgcgttcg tactcccgca gtttccggat gctctccgaa tcctcggaac cttttacgga
     4621 ataggtctcg tcaagtcgct gggcgtctcc gttgatctcc accgtttcac cgcctttcag
     4681 aatgagaaac aacaggttgg tcggattcgc gcgcaggatg taatagtcca gctcggtcac
     4741 cggattgcga aagctgaact tcccgtcggc atcggtcgtc acggaatcca
//
LOCUS       DAOTON010000099         4711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_14940_length_4711_cov_3.184493, whole genome shotgun sequence.
ACCESSION   DAOTON010000099 DAOTON010000000
VERSION     DAOTON010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14940_length_4711_cov_3.184493"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="P5292_03000"
     CDS             <1..442
                     /locus_tag="P5292_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRS38124.1"
                     /translation="LFLESVDAKKDLQIYMNSPGGSVYAGLGIYDTMQLVQPSIGTIC
                     TGMAASMAAVLMCAGDKGKRAALKHSRIMIHQPLGGAEGQASDIEITAREIQKLKKEL
                     YEIIASHSGQEYEKVWKDSDRDYWMTAEEAKAYGMVDEVLTRKK"
     gene            550..1791
                     /gene="clpX"
                     /locus_tag="P5292_03005"
     CDS             550..1791
                     /gene="clpX"
                     /locus_tag="P5292_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRS38125.1"
                     /translation="MKNPKEIKCSFCGRDKSETYVLIAGITGHICDQCIQQAQNILND
                     EMNSKLKTSLNSHLTLLKPVEIKKFLDQYVIGQDEAKKVLAVAVYNHYKRISSKIKKQ
                     DEVEIEKSNILLVGETGTGKTLLARTIAKMLNVPFCIADATVLTEAGYVGEDVESILT
                     RLLQAADYDVPSAERGVVYIDEIDKIARKGDNPSITRDVSGEGVQQALLKLLEGSVVN
                     VPPQGGRKHPEQKMISMDTSNILFICGGAFDGIDKKIARRLETHAIGYKSSKDAEKID
                     RSNYLQYISPTDLKAFGLIPELIGRVPVLTHLDPLDKPALKAILTEPKNALIKQYEKL
                     FQLEGVSLSVEKGVLDFIVEKAFEFKLGARGLRSICEAILLDAMFEVPSNKQGIKEFT
                     LTLAYAKEKFSKANINRLKVA"
     gene            2048..2446
                     /gene="rpsL"
                     /locus_tag="P5292_03010"
     CDS             2048..2446
                     /gene="rpsL"
                     /locus_tag="P5292_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRS38126.1"
                     /translation="MPTISQLVRKGREKLIDKSKSPALTSCPQRRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNKYEVTAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGSL
                     DTAGVEGRKQQRSKYGTKRPKPGAAPAGKK"
     gene            2483..2950
                     /gene="rpsG"
                     /locus_tag="P5292_03015"
     CDS             2483..2950
                     /gene="rpsG"
                     /locus_tag="P5292_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRS38127.1"
                     /translation="MRKTKPKKRILLPDPKFNDTAVTRFVNMMMLSGKKNTAFAIFYD
                     AVQKVEDKTKENGLDTWKKAMANVMPTVEVKSRRVGGATFQIPTEIRPDRRISLSIKW
                     MINYSRLRNEKTMSDRLAGEIIAAAKGEGAAVKKKEDTHRMAEANKAFSHFRA"
     gene            3161..>4711
                     /gene="fusA"
                     /locus_tag="P5292_03020"
     CDS             3161..>4711
                     /gene="fusA"
                     /locus_tag="P5292_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRS38128.1"
                     /translation="MSKELTNTRNIGISAHIDAGKTTTTERVLYYTGKVHKIGEVHEG
                     AATMDWMVQEQERGITITSAATTTYWNYLNDQYKINIIDTPGHVDFTVEVERSLRVLD
                     GAVALFCAVGGVEPQSETVWRQMNKYRVPRIGFVNKMDRAGANFLNVVAQVKERLSGN
                     PVPLQLPIGAEDMFKGVVDLITNKAIIWHEETKGATFDVVPIPVDMQADVNHWRANLV
                     EAVAEFDDHLMEKFFEDPNSITEDELRTAIRKAVIAMKITPMMCGSAFKNKGVQAMLD
                     AVIQYLPAPNEIENVKGVNPNTDQEEERKADPKDKFAALAFKIMTDPFVGRLAFFRVY
                     SGRLDAGSYVLNARTGKNERISRIIRMHANKQNPIDFIEAGDIGAAVGFKEIKTGDTL
                     CDVNAPIILESMSFPTPVIGLAIEPKTQADVDKLGMALAKLAEEDPTFRVHTDEETGQ
                     TVISGMGELHLEIIVDRLKREFKVECNQGAPQVAYKEAIMGSASHRETYKKQTGGRGK
                     FADIQFEIS"
BASE COUNT         1239 a         1190 c         1286 g          996 t
ORIGIN      
        1 cctttttctg gagtcggtgg atgccaagaa ggatcttcag atctatatga acagtccggg
       61 cggatccgtt tatgcaggtt tgggcatcta tgataccatg cagttggtac agccgagcat
      121 tggtaccatt tgcaccggca tggccgcatc gatggccgcg gtattgatgt gtgccggcga
      181 taaaggcaag cgggcagcgt tgaagcactc ccggatcatg atccaccagc cgctgggcgg
      241 cgcagaaggt caggcgtccg atatcgaaat cacggcacgg gagatccaga agctgaagaa
      301 agaactgtac gagatcattg cttcgcattc gggtcaggag tacgagaaag tgtggaaaga
      361 ttccgaccgc gattactgga tgaccgccga agaagccaag gcttacggca tggtcgatga
      421 agtactgacg cgaaagaaat aagcggtaaa accgcatttt ttaaacgcaa gttttaaggt
      481 gttcttgttt gacccggtcg aacaaaattc cttagcttgc gtttaatttt tcgcggctct
      541 ccatccaaca tgaagaatcc aaaagagatc aaatgttcct tctgcggcag ggacaagagt
      601 gagacctatg tgctgattgc gggcataacg ggtcatattt gcgaccagtg catccagcag
      661 gctcagaata ttctgaatga cgagatgaac agcaagttga aaacttcgct gaattcccat
      721 ctcacgctgc tgaaacctgt cgaaataaag aagttcctgg atcaatacgt gatcggtcag
      781 gacgaagcca agaaggtatt ggccgtcgcc gtttacaacc actacaagcg catttccagc
      841 aagatcaaga agcaggacga agtagagatc gagaaatcga acatattact cgtcggtgaa
      901 acgggtaccg gcaagaccct gctggcccgt acgatcgcta aaatgctgaa tgtgcctttc
      961 tgcatcgcgg atgccaccgt attgacagag gcgggctatg tcggggaaga cgtagaaagc
     1021 attcttaccc gtttattgca ggcagccgat tatgacgtac catcggcgga gcggggcgtg
     1081 gtgtacatcg acgagatcga taaaatcgcc cggaaaggcg ataatccgtc cattacccgg
     1141 gatgtaagcg gggaaggggt gcagcaagcc ttgttgaaac tcctggaagg ttccgtggtg
     1201 aatgtccccc cgcaaggagg ccgcaagcat ccggagcaga agatgatctc gatggatacc
     1261 tccaatatcc tgttcatctg tggaggcgcc tttgacggga ttgacaaaaa gatcgcccgg
     1321 cgtttggaga cgcacgcgat cggctataaa tcgtccaaag acgctgaaaa aatcgaccgg
     1381 tcgaattatc tgcaatatat cagtccgacc gacctgaagg ccttcggact cattccggag
     1441 ctgatcgggc gcgtgccggt attgacccac ctggatccgc ttgacaagcc tgccttgaag
     1501 gcgatcctga ccgagcccaa gaacgcgctc atcaagcaat acgagaaact gttccaatta
     1561 gagggtgtaa gcctgtcggt tgagaagggt gtacttgact tcattgttga aaaagcgttc
     1621 gaattcaaac tgggagcccg cggcctgcgg tcaatctgcg aagccattct tttggacgcc
     1681 atgtttgaag ttccttctaa caagcaggga atcaaggaat ttacgctcac tttggcctat
     1741 gccaaggaga aattctccaa agcgaacatc aaccgcctga aagtagctta aatagctgaa
     1801 aatcaacaga aaagggctcc gcatgccggg gcccttttta ttttattgat aatgagcgat
     1861 ttgcagattg actggggaat agccgggtag ccagtcagcg gcggccttgt gttgagccgt
     1921 gaaaatgtgt gaaatatttt atgaacagca tttggtaaat agggagtttg tggtaccttt
     1981 gcattcctca aaatcaggcg aaaagcgcct aatagactga acgttaaccc gaaagccagt
     2041 tacccgaatg ccgacaattt cacagttggt ccgtaaggga cgcgaaaaat tgatcgataa
     2101 gagcaaatca cctgctctta cctcgtgtcc gcagcgtcgt ggtgtttgca cccgcgtgta
     2161 taccaccact cccaagaagc cgaattccgc catgcgtaag gtggcgcgtg ttcgtctgac
     2221 caacaagtat gaagtgacgg cctacatccc gggtgagggt cacaacctcc aggagcactc
     2281 catcgtgttg atccgcggcg gtcgtgtgaa ggatcttccg ggtgttcgct accacatcgt
     2341 tcgtggttct ttggacacgg ctggtgttga gggtcgcaag cagcagcgtt cgaaatacgg
     2401 aaccaagcgt ccgaagccgg gtgctgcacc tgcaggtaag aagtaatcag aaataacgaa
     2461 tagcgtatag ctgcatcatc ccatgcggaa gacaaaaccg aaaaaacgca tcctgttacc
     2521 ggatcccaag ttcaacgaca cggcagtaac ccgtttcgtg aacatgatga tgttatccgg
     2581 taagaagaac acggcattcg ccattttcta cgacgccgtc cagaaggtag aagacaagac
     2641 gaaggagaac ggactggaca cctggaaaaa agccatggcg aacgtgatgc cgacggtaga
     2701 agtaaagagc cgtcgtgtcg gcggagctac cttccagatc ccgacggaaa ttcgtccgga
     2761 ccgccgcatc agtctttcga tcaagtggat gatcaattac tcccgcctgc gcaacgagaa
     2821 gaccatgtcg gatcgtctgg ccggtgaaat catcgccgct gcaaagggtg aaggtgcagc
     2881 tgttaagaaa aaagaagaca cgcaccggat ggcagaagcc aacaaggctt tctcgcactt
     2941 ccgtgcctga taatacgaat gcccgggccg acaacggtcg gtccaactcg gatagttctt
     3001 tctttaacgg gataaccccc tgacttgccc agcatggcaa ggtaacagac tggataactg
     3061 agcggaggac gtctccgaca agcgcgcagg cgccgctcaa agtgtccggt tatcgcggaa
     3121 tagaaacaac tatcgttatg attaacggat aagtaatcaa atgagcaaag agctaacaaa
     3181 tacccgcaac atcggcatct cggcacacat tgacgccggg aagacgacta ctacggaacg
     3241 tgtcttgtat tacaccggta aggtgcacaa gatcggtgag gtacacgaag gcgctgctac
     3301 catggactgg atggttcagg agcaggagcg tggtatcacc attacctccg cagctaccac
     3361 tacttattgg aattacctga atgaccagta taagatcaat atcattgata cacctggtca
     3421 cgttgacttt accgttgaag tagagcgctc cctgcgcgta ctcgacggcg ctgtcgccct
     3481 tttctgtgcc gttggtggtg tggagcccca gtcggagacc gtttggcgtc agatgaacaa
     3541 ataccgcgtt ccccggatcg gtttcgtgaa caagatggac cgtgccggcg cgaacttttt
     3601 gaacgttgtc gctcaggtaa aagagcgcct cagcggaaat cccgttcccc tgcagctccc
     3661 gatcggcgcc gaagacatgt tcaaaggcgt ggtcgacctg atcaccaaca aagccatcat
     3721 ctggcatgaa gagaccaaag gcgctacgtt cgacgtagta ccgatcccgg tcgacatgca
     3781 agccgatgtg aaccactggc gcgccaacct ggttgaagcc gtggctgaat tcgacgacca
     3841 cctgatggag aagttcttcg aagatccgaa cagcatcacc gaagacgagc tccgtacggc
     3901 tatccgtaag gcggttattg ccatgaagat caccccgatg atgtgcggtt ccgccttcaa
     3961 gaacaagggt gttcaggcga tgttggacgc agtgatccag tacctcccgg ctccgaacga
     4021 gatcgagaac gtgaagggtg tcaaccccaa cactgaccag gaggaagagc gtaaggctga
     4081 tccgaaggac aaattcgcag cacttgcgtt caagatcatg accgatccgt tcgtaggtcg
     4141 tctcgcgttc ttccgtgtgt attccggtcg cctggatgca ggttcgtatg tgctcaacgc
     4201 acgtaccggc aagaacgagc gtatctcgcg tatcatccgc atgcacgcca acaagcagaa
     4261 cccgatcgac ttcatcgaag ccggtgatat cggtgctgcc gttggtttca aggaaatcaa
     4321 gaccggtgac acgctgtgcg atgtcaacgc gccgatcatc ctcgaatcga tgagcttccc
     4381 gaccccggtt atcggtctcg ccatcgagcc gaagacccag gccgacgtag acaagctggg
     4441 tatggcgctt gccaaacttg ccgaagaaga tccgacgttc cgcgtacaca ccgacgaaga
     4501 gaccggtcag accgttatct ccggtatggg tgaactccac ctggagatca tcgtggaccg
     4561 tctgaagcgc gagttcaaag tagaatgtaa tcagggtgca ccgcaggtgg cttacaaaga
     4621 agccatcatg ggcagtgctt cgcaccgtga gacgtataag aagcagacgg gtggtcgcgg
     4681 taagttcgcc gacatccagt tcgaaatttc t
//
LOCUS       DAOTON010000100         4702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_14977_length_4702_cov_2.971595, whole genome shotgun sequence.
ACCESSION   DAOTON010000100 DAOTON010000000
VERSION     DAOTON010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14977_length_4702_cov_2.971595"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="P5292_03025"
     CDS             complement(<1..1382)
                     /locus_tag="P5292_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HRS38129.1"
                     /translation="MEFSRPFELIDLPGNETAPDSLHYTLRFKEDKSNELLVKVALSS
                     VSEANARMNLERELPGWDFVATKSAARAAWNRELGKIEAKGGSEKQLRIFYTALYHTM
                     IVPNVYSDVDGRYRGRDGQVHAIDGPEQYTVFSLWDTYRAWHPLMTIIDRKRTTAYVN
                     TFLRQYREGGLLPVWELSANETECMIGYHAVPVIADADAKGIGGFDRMQALMAMRKSA
                     EAKERYGLGAYMKHAMLSLDDEHESVSKTLEYAYDDWCIATMAQRLGQGPLAQQYFER
                     SQSWKNLLNPVTGFMQPRVNGGWLSPFDPYEVNNNFTEANSWQYSFYVPQDIPGLMRI
                     HGGMERFAARLDSLFAADNRTRGREQADITGLIGQYAHGNEPSHHMAYLYNYCGQPWK
                     TQRLVRKIMDEFYQASPDGLIGNEDCGQMSAWLVFSAMGFYPVTPGAPYYAIGTPWFQ
                     EVTIHLEDGKS"
     gene            complement(1355..1978)
                     /locus_tag="P5292_03030"
     CDS             complement(1355..1978)
                     /locus_tag="P5292_03030"
                     /inference="COORDINATES: protein motif:HMM:NF037611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38130.1"
                     /translation="MKKILFSLFACLSFQANAQVDLARHVDPFIGTGGHGHTYPGATL
                     PFGMVQLSPDTRVDGSWDGCGGYHHSDSMMYGFSHTHLSGTGCSDYGDILLLPYVNKL
                     DGTPIQPVRFSHADELAEPGYYSVDLPTVGVKTEFTTTVRVGFHRYAFNGVGEHRVLL
                     DLRHRDKVLGAEMRMVSDRRIEGYRRSSAWAGIRSSISPWSFLGHSN"
     gene            complement(1985..4516)
                     /locus_tag="P5292_03035"
     CDS             complement(1985..4516)
                     /locus_tag="P5292_03035"
                     /inference="COORDINATES: protein motif:HMM:NF036482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HRS38131.1"
                     /translation="MKKLFAFALVCCTVFLSEALARPGKFVWTLGGQWEFRQQDSTEW
                     WPAIVPGTVHRDLLRAGRIPDPYFGTNESRLGWIDSVAWIYRCRFDASAELLSRRNIE
                     LVLDGLDTYATVSLNGVELLTADNMFRQWSVAVKSVLREKKNELLIRFKPAILVARQQ
                     AEKTDIVIPGEDRIYARKAAYHFGWDWGARFIGCGVYSPVHLDASDDFQYEATRFTIE
                     RLDSAVAVVNFTTEVQVAVPGRYHLRVAGGKGSESVYEVWSDLNAGKQQLSVSGRIEQ
                     PRLWWPNRSGGHPLYPFRITVSDSRGDAVEDSLLVGLRSIRLQQDVDSVGSAFTVVVN
                     DRPLFMKGANIIPPDHFSTMSDDSSWVALVRLANETGMNMLRVWGGGIYPPNAFYNAC
                     DSLGILVWQDFMFACSMVPLDDEFRKNVEQEAIGQVRRLRNHPSLALWCGNNESDEGW
                     NNWGWQRQYGYTPEDSMRVKAAYDCLFHDVLPGVVKSNDDRPYHPSSPATGWGRSAAY
                     RVGDVHYWGVWWGHAPFATYEDHYGRFVSEYGFQGLPCMATIREFAGSEVLDASKGAL
                     AAHQKHPTGYQTITEYMKARYRDPKDIQSYAHVSQLLQADALVTAIDAHRGAMPLCMG
                     SLYWQLNDTWPVVSWSTVDHTGRKKAAHWAVTEAFSTVRLLPRVDGQRLEVRVVNDSA
                     TDLLLRMELTLIDFHGNIRWSEVAAADAKSSAVSRVYSSNLAPVLRFMDTCDAMLRIR
                     LVSHDEVISQRDVFFTSPRNLKLYRPAMSIKVIPGPVGAQYIELRSSVLALGVELSVD
                     GDPFWFEENYFTLLPKENYRIRIVERPGKTFDSTRLRFRSLVDTY"
     gene            complement(4513..>4702)
                     /locus_tag="P5292_03040"
     CDS             complement(4513..>4702)
                     /locus_tag="P5292_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38132.1"
                     /translation="AGAGVSADHARQLVEESGVKEIHLSAKRSRVRRLPAAHALTEDR
                     PDGVSDPSFIRLLKQALG"
BASE COUNT         1149 a         1381 c         1161 g         1011 t
ORIGIN      
        1 gacttaccat cctcgagatg gatcgtcacc tcctgaaacc agggcgtacc gatcgcatag
       61 tagggagctc cgggtgtcac gggataaaat cccatcgcgc tgaacaccag ccaggccgac
      121 atttggccac aatcctcatt gccgatcaat ccatcggggg aagcctgata gaactcatcc
      181 atgatcttgc gcaccaatcg ctgtgtcttc cagggttgac cgcaataatt gtagaggtaa
      241 gccatgtgat gactcggctc gttcccgtgt gcatattgtc cgatcagtcc ggtgatatcg
      301 gcctgttccc gaccccgggt ccggttatca gcagcgaaaa gcgaatccag tcgtgctgcg
      361 aatcgctcca ttcctccgtg tatccgcatc aacccgggaa tatcctgcgg aacataaaac
      421 gaatattgcc aggagttcgc ctcggtaaaa ttattgttga cttcatacgg atcgaaggga
      481 gacaaccacc caccgttaac ccggggttgc ataaaaccgg tgaccgggtt cagcaggttc
      541 ttccaggatt gcgatcgttc gaaatactgc tgcgccaacg gcccctgacc aagccgttgc
      601 gccatggtgg caatgcacca atcatcatat gcatactcca gcgtcttgga aaccgactca
      661 tgctcatcgt ccagggagag catcgcatgt ttcatgtaag ccccgaggcc gtagcgttct
      721 ttggcttcag cgcttttacg catggccatc agggcctgca tgcggtcaaa accgccgata
      781 cccttcgcat cggcatcggc aatcaccggc accgcgtgat aaccgatcat gcattcggtc
      841 tcattcgcgg acaactccca gacgggtaat aatccgcctt ccctgtactg ccggagaaac
      901 gtgttcacat aagccgtagt tcgcttccgg tcgataatcg tcatcaaggg atgccatgcc
      961 cggtaagtgt cccacaacga gaaaaccgtg tattgttccg gcccatcaat ggcatgcacc
     1021 tgaccatcac gaccacgata gcgaccatct acatcggaat agacgttggg gacgatcatg
     1081 gtatggtaga gggccgtgta gaaaatccgg agttgctttt cacttcctcc ctttgcttcg
     1141 atcttaccca actcccggtt ccaggcagcg cgtgccgcgc ttttcgtcgc gacgaaatcc
     1201 cagcccggca actcgcgctc aaggttcatt cgggcgttgg cttcgctcac ggaagaaagg
     1261 gcgaccttca ccaggagttc gtttgatttg tcttctttaa agcgcaatgt gtagtgcaaa
     1321 ctatcgggag ctgtttcgtt gcccgggaga tcgatcagtt cgaatggccg agaaaactcc
     1381 atggagaaat agacgacctg atccccgccc aggcggaaga gcgccgatag ccttcaattc
     1441 gtctatcgct gaccatccgc atttccgcac caaggacttt atcacgatgg cgcaggtcca
     1501 gcaggacccg gtgttcacca acgccattga aagcataacg atgaaacccg actcgcacgg
     1561 tggtggtaaa ttcggttttg acacctacag tcggaagatc cacgctgtag tatcccggtt
     1621 ccgccaattc atccgcgtgt gaaaatcgca cgggctggat cggagttcca tctaacttgt
     1681 tgacatacgg cagcagcagg atatcgccat aatccgagca tccggtcccg ctcaaatggg
     1741 tatgagaaaa tccatacatc atggaatccg aatgatggta accgccgcaa ccatcccagg
     1801 aaccatcaac gcgcgtatcg gggcttaact gcaccattcc gaaaggcagg gtcgcaccgg
     1861 gataggtatg accatggccc cctgtcccga tgaacggatc aacatggcgg gcgaggtcga
     1921 cctgggcgtt ggcctggaat gagagacagg cgaataagct gaaaagaatc ttcttcatgt
     1981 tcgtttaata cgtatcaacc agggatcgaa agcgtaaacg tgtgctgtcg aatgtcttcc
     2041 ccggtctttc cactattcga atgcgataat tctcctttgg caacaaggta aaatagtttt
     2101 cctcaaacca gaaaggatcc ccgtccacac tgagttcgac acccagcgca agaacactgg
     2161 agcgaagctc aatgtattgc gctcctaccg gcccgggtat gaccttgatc gacatggccg
     2221 gccggtataa tttcaaattc ctgggtgaag taaagaaaac gtcacgctgg ctgatgacct
     2281 catcgtgtga caccaagcgg atcctcaaca tggcatcgca ggtatccatg aatcgcagta
     2341 cgggcgcgag attggaggaa tacacccgac tgacagcgga cgactttgca tcggcagcgg
     2401 ccacttcgct ccaacggata ttcccatgga aatcgatcag cgtgagctcc atccgcagca
     2461 ggagatcggt cgctgaatca ttcacgaccc gaacttccag tcgctgccca tccacccgtg
     2521 gtaagagtcg aacggtactg aacgcttcgg tgaccgccca gtgtgctgcc ttcttgcggc
     2581 cggtatgatc caccgtactc caggaaacca ccggccaggt atcgttcagt tgccagtaca
     2641 acgaccccat acaaagcggc atggcgcctc gatgtgcatc aatcgccgtg acaagagcat
     2701 ccgcttgcaa caattgagat acatgcgcat acgattgaat gtcctttggg tcgcggtacc
     2761 gcgctttcat gtattcggtg atcgtctgat agccggtagg atgcttctga tgtgccgcca
     2821 gcgcgccttt ggatgcatcc aacacttccg aacctgcgaa ctcacgaatg gtcgccatgc
     2881 agggaagccc ttgaaaacca tactcactga cgaaccggcc ataatgatct tcataggtag
     2941 cgaacggagc atgtccccac caaacgcccc aataatgcac gtcccctacc cgataagccg
     3001 cactacgtcc ccaacccgtt gccggggaag aggggtgata gggacgatcg tcatttgatt
     3061 tgaccacacc gggaagcaca tcatgaaaca gacaatcata ggccgccttg actcgcatcg
     3121 agtcttccgg tgtgtatccg tattgacgct gccagcccca gttgttccaa ccttcgtccg
     3181 attcattgtt gccacaccac aaggcaagac tcggatgatt ccgaagacga cgtacttgcc
     3241 cgatcgcttc ctgctcgaca tttttcctaa actcatcatc caacgggacc atggagcagg
     3301 cgaacatgaa atcctgccat accagaattc caaggctatc acaagcattg taaaacgcat
     3361 tcggcggata gatccctcca ccccaaacgc gtagcatatt catacccgtt tcattcgcca
     3421 gtctcaccaa agccacccag gatgaatcat ccgacatggt actgaaatga tccggaggga
     3481 tgatgttggc acctttcatg aacaatggac gatcgttcac gaccacggtg aacgcactac
     3541 cgacactatc cacgtcctgc tgaaggcgga tcgagcgtaa accgaccagg agggaatctt
     3601 cgaccgcatc gcctctgctg tcggacacgg taatacgaaa aggatacaac ggatggccgc
     3661 cacttcggtt gggccaccag agcctgggct gctcgatcct tccacttacg gaaagttgtt
     3721 gcttccctgc attcagatca ctccacactt cataaacgct ttcggaccct ttccctccgg
     3781 caacacgaag gtgataccgt cccggaaccg cgacttgtac ctccgtggta aaattcacaa
     3841 cggccacagc ggaatctaga cgttcgatcg tgaacctggt ggcttcatac tgaaaatcat
     3901 ccgaagcatc gaggtgaacc ggactataga cgccgcatcc aatgaaacgg gctccccaat
     3961 cccaaccgaa atgataagcg gctttacgcg cataaattcg gtcttcccca ggaatgacga
     4021 tgtctgtttt ctccgcctgc tgacgcgcga ccagtattgc cggcttgaat ctgatcaaca
     4081 attcattctt cttctcccgg agtactgact ttacggcgac gctccactgc cggaacatat
     4141 tgtcggcagt aagcaattcg actccgttca ggctgacagt cgcatacgta tccaagccgt
     4201 caagtaccaa ctcgatattt ctgcgcgaca acaactcggc gcttgcatca aagcggcatc
     4261 ggtaaatcca ggcaacgctg tcaatccagc caagacgaga ctcgttcgta ccgaaataag
     4321 ggtctggaat tcttcctgca cgcaagaggt cgcggtgtac cgtgccggga acaattgccg
     4381 gccaccattc ggtagaatcc tgctgacgga attcccactg tcctcccagt gtccaaacaa
     4441 acttacccgg gcgggccaat gcctccgaca ggaacaccgt gcagcagacc agggcaaacg
     4501 cgaacagctt cttcatccaa gggcttgttt cagcaaccgg atgaaggaag ggtccgaaac
     4561 cccatccggg cgatcttccg tcaatgcgtg cgcggcaggc agtcgtcgta cgcgcgatcg
     4621 ctttgcggaa aggtgaattt ccttcacgcc tgattcttcc acgagctgac gagcatggtc
     4681 cgcggaaaca ccggcacctg cg
//
LOCUS       DAOTON010000101         4696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15008_length_4696_cov_4.054945, whole genome shotgun sequence.
ACCESSION   DAOTON010000101 DAOTON010000000
VERSION     DAOTON010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15008_length_4696_cov_4.054945"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="P5292_03045"
     CDS             <1..908
                     /locus_tag="P5292_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS38133.1"
                     /translation="FNDLIPEADRVYVVIGSLIVILGLLFYAMGAAAKNKQGWGAMQY
                     PQLVLGMIAIFVYVGMEVTVQSNMGALLKQPEFGGLDEKYISQFISLYWGSLMIGRWT
                     GAVAVFKVSPSVKKLLTVIVPLIAFGVILFVNHLRGNDVENLYVYVLCVLVLIAAFLY
                     ANEQPVRMLFTVAALGTLALLVGLFTTGTVSTYAFISAGLCCSVMWPCIFALAIMGLG
                     KYTSQGSAFLIMMILGGAIIPPAQGVICDLDKHAPGGILGMSYTHFSYVLPLLCFIYL
                     AWHALRTSGILKAQGLQVETPAAGH"
     gene            complement(914..1987)
                     /locus_tag="P5292_03050"
     CDS             complement(914..1987)
                     /locus_tag="P5292_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38134.1"
                     /translation="MMNRLYALLLAMLPLLAIGQSYNGPESAEFDYVNRRWLIGNTSS
                     HQILSRDSLGNLSVLVNSTTSGPYGIEIVGDTVFCCSGGSIKGYLLSDGTPVYNLNVG
                     GSFLNGLTHDNAGNLFATDFSTKSIIRVHIASQTFAPVATSLPNTPNGIVFDGYNNRC
                     VFVNWGSNAPIRAIDLATYAVTTVVTTTLGSCDGITRDAIGRYYVSNWSGQSIVRFDS
                     AFAAPPATVVSGLSNPADIFYNVVDDTLAIPNAGNNTVTFYGFPSAVGISNVMPEHLV
                     RMGPNPLPVGGTLVVHCPSRVSGVKLHNLNGALIGTPVCEQRDLETVLILHMPTGLSG
                     GTYYLSFITDAGVIRKKFSMIDP"
     gene            2073..3938
                     /locus_tag="P5292_03055"
     CDS             2073..3938
                     /locus_tag="P5292_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38135.1"
                     /translation="MRHTFLIAVCIGLFLHSHGQGTLPKERSAEIVQKIIREKLRPGF
                     LDNPEVRKHLPATLQAEFQQEARTANITEVVVSGGSEAESEVHATGSPQDSTILIVSS
                     NTVDPNGSGATDVPVNHIYYSHDFGSTWQRSAFDPLPVTSGVTIAGGGDPNFCYDMNG
                     RIYTSWINLYLQGFTSVTWDLLWAYSDDDGVTWQRGTDPAIISTTGPITALFDPTQFN
                     GPIADKEWLAVDRGFSSYANTVYCVYYEAGGPANLNAIAVRHLSPGSGSFSSSTYVTD
                     NSFVFVQFGSIDVAANGRVHVSFFGSTDNVNFGVYHAYSDDGGISFSAPQLVSETQIP
                     NFSPGQSGQTIAGINVDRMYPCVYLAVDKSYMQTNSGNAYLVWSANGVNAQGTTGMDI
                     YFSKSTDEGQTWSPAIVLNNTAPGDASDQFYPNLAVSYGGIVAVGWYDRRNHPGTANT
                     DYYMAWSFDGGTSFNNDIPVSDAPTDFNTIGSVNQDFGIGEYNGLVVTENYGIPVWGD
                     GRTNDGELDLVAALVPFRFNTGLPAHTGSIASMGIAPNPAGSFSRVEVNLLTAGTYRL
                     QIVDARGRICTTSTLQLSAGVSRIPIETDHLANGHYTILLRNDKGLSQAELVINH"
     gene            3956..>4696
                     /locus_tag="P5292_03060"
     CDS             3956..>4696
                     /locus_tag="P5292_03060"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRS38136.1"
                     /translation="MHTFRTFLLFILAFTLNTTAQTGTPLDPTLDQSVKEGLLQNPND
                     KGLQLIQSQQYSTAETFLNNSLKENDLDPKAYFQRGVVNWQQSDTLAACRDWSSVLAL
                     GDTATYILLTKNCGGNVHFGDEVMPVKTFRRALVTKPEPVMSAAEADKQTERYVDQLP
                     EFPGGTEGLFGYLKKNLNTAAVKDGSAGTLAFVEFIIDRKGKVLYPHVVRGANKDLNT
                     EAVRVIRNMPAWKPATKKGQPVLVRYTLP"
BASE COUNT         1075 a         1306 c         1307 g         1008 t
ORIGIN      
        1 tcttcaacga ccttatacct gaagcggacc gtgtatatgt ggtcatcggt tcattgatcg
       61 taatacttgg cctgctcttc tacgccatgg gggcagccgc gaagaacaag cagggctggg
      121 gcgccatgca atacccgcaa ctggtgctgg gcatgatcgc gatcttcgtg tatgtcggga
      181 tggaagtgac ggtgcaaagc aatatgggcg cgttgctgaa acaaccggag ttcggcgggc
      241 tcgacgaaaa atacatttcg cagttcatct cgctctattg gggtagtctc atgatcggtc
      301 gctggaccgg cgcggtggcg gtattcaaag tgtcgccctc ggtaaagaaa ttgctgaccg
      361 taatcgttcc gttgatcgcc ttcggggtca tcctgttcgt gaaccacctg cgcggtaacg
      421 atgtggagaa cctgtacgtc tatgtactgt gcgtacttgt cctgatcgcc gccttcctct
      481 atgcgaacga acagccggtt cgcatgctct ttacggtcgc tgcgcttggg accctcgcgc
      541 tgcttgtcgg attgtttacg accgggacgg tgagcacgta cgccttcatc agcgcgggct
      601 tgtgttgctc ggtcatgtgg ccctgcattt tcgcattggc gatcatggga cttggaaaat
      661 acaccagcca gggttcggca tttttgatca tgatgatcct ggggggcgcc atcattccgc
      721 cggcgcaagg tgtcatctgt gatctggaca agcatgcgcc gggagggatc ctgggcatga
      781 gctacacgca cttctcgtac gtacttccgc tactctgttt catctacctg gcctggcatg
      841 ccctgcgtac gtcgggtatc ctgaaagcgc aggggttgca ggtggagacg cctgcagccg
      901 gacattgaac cggtcaggga tcgatcatgc tgaacttctt ccggatcaca ccggcatcgg
      961 tgatgaagct gaggtagtac gtacctcccg aaagtcccgt cggcatatgc aatatcagaa
     1021 ccgtttccag atcgcgctgt tcgcagaccg gcgtcccgat cagggctccg tttaagttgt
     1081 gcagctttac gccggaaaca cgcgaaggac aatggacgac cagggtgccg ccgacaggca
     1141 ggggattcgg acccatgcga accagatgtt ccggcatcac attgctgatt cctaccgccg
     1201 atggaaatcc ataaaaagtg accgtattat tccccgcgtt gggaatcgcc agcgtatcat
     1261 ccaccacatt gtaaaagata tcggccggat tggaaagtcc gctcaccacc gttgcaggag
     1321 gagcagcgaa ggcgctatcg aagcgcacaa tggattgacc gctccagtta cttacataat
     1381 aacgaccgat cgcgtcgcgg gtgatgccgt cgcagctacc aagcgtcgtg gtaacaaccg
     1441 tggtgacggc atacgtggca aggtcgatcg cacggatcgg cgcgttacta ccccagttca
     1501 cgaagacgca acgattgttg tatccgtcga agacgattcc gttgggcgta ttcggcaacg
     1561 aagtggcaac cggcgcgaaa gtctgcgatg cgatgtgaac gcggatgatg cttttcgtcg
     1621 agaaatccgt tgcaaaaaga ttgcccgcat tgtcgtgggt caatccgttc aggaaacttc
     1681 cgccgacatt caggttgtaa accggcgttc catcggaaag cagataccct ttgatactgc
     1741 cgccgctgca acagaacacc gtatcgccga cgatctcgat tccgtacgga ccactggtgg
     1801 tggagttgac caagacgctc aggttgccca ggctgtcgcg gctcaggatc tggtgactgc
     1861 tcgtatttcc aatcagccaa cggcggttga cgtaatcgaa ttcggcgctt tcggggccat
     1921 tataggattg gccgatggcc aggagcggga gcatggccag caagagtgcg tagaggcggt
     1981 tcatcatggc ggcaagatag agaatccgac gaccggcttc gcaagggtga aatcaggaaa
     2041 acattatctt gtaggaccat ctccacagca ccatgcgcca tactttcctg atcgccgtct
     2101 gcattggcct ttttcttcat tcccatggcc agggtacgct cccgaaggaa cgctccgcgg
     2161 aaatagtcca gaagatcatc cgcgaaaaac ttcgtccggg atttctggat aatccggagg
     2221 tccggaaaca tctgccggcc accctgcaag ccgaatttca gcaagaggcc cgaaccgcga
     2281 atatcaccga agtggtggtg agtggcggaa gtgaagcgga gtcggaagtg catgccaccg
     2341 gcagccctca agacagcacc atcctgatcg tttcttccaa tacggtcgac cccaacggtt
     2401 ccggcgcaac cgatgttcct gtcaatcaca tttattatag ccatgatttc ggttccacct
     2461 ggcaacgcag cgcattcgat ccattgcccg taacaagcgg cgtcaccata gccggaggcg
     2521 gggatccgaa tttctgctac gacatgaacg gcaggatcta taccagttgg atcaatttgt
     2581 acttacaggg tttcacctcc gtcacctggg acctgctctg ggcctattcc gacgacgatg
     2641 gcgtgacctg gcagcggggc accgatcccg ctattatcag tacgacgggt ccgattactg
     2701 cactgttcga tcccactcag ttcaacgggc cgatcgcgga caaagaatgg ctcgctgtcg
     2761 atcgtggatt ttcatcgtac gcgaacacgg tgtactgtgt ttactatgaa gccggaggcc
     2821 ccgcgaacct taacgcgata gccgttcggc atttaagccc cggttcgggg agtttttcgt
     2881 cttccacgta tgtgaccgat aattcgttcg tcttcgtcca gttcggctcc atcgatgttg
     2941 ccgccaacgg gagagtacac gtttcgtttt tcggatcaac ggacaatgtc aatttcgggg
     3001 tgtatcatgc ttactccgac gacggcggca tcagcttcag cgcaccacaa cttgtgagcg
     3061 agacacaaat acccaacttc tcacccgggc aatcggggca gacgatcgcc ggcatcaacg
     3121 ttgatcgcat gtacccgtgc gtgtacctgg ccgtggacaa atcctacatg cagaccaatt
     3181 ccgggaatgc atatctggtc tggtcggcaa atggtgtgaa cgcgcaggga acgacgggca
     3241 tggacattta cttcagcaag tcgaccgatg aaggacaaac ctggagcccg gcgatcgtgc
     3301 ttaacaacac ggcacccgga gacgcctccg atcaattcta cccgaacctg gcggtgagct
     3361 acggagggat cgtcgcggtc ggttggtacg accgacggaa ccatcccggc acggccaaca
     3421 ccgactatta catggcctgg tcgttcgacg gcggaactag tttcaacaac gacattcccg
     3481 tcagcgacgc cccgaccgat ttcaatacga tcgggtccgt caatcaggat ttcggtatcg
     3541 gagaatacaa cggactcgtc gtgacggaga actacggcat tcccgtatgg ggtgatggca
     3601 ggacgaacga cggcgaattg gatctggtcg cggccctggt accctttcgt tttaacaccg
     3661 gacttcccgc tcatacgggc agcatcgctt ccatgggcat tgcacccaat ccggccggca
     3721 gcttcagccg ggtggaggtc aacctcctta ctgccggcac gtatcgcctg caaatcgtgg
     3781 atgctcgtgg acgcatttgt acaacaagca ccttgcaact ctccgccggt gtcagcagga
     3841 ttccgatcga aaccgatcat ctcgcaaacg gccactacac gatactgctg cggaatgata
     3901 aagggttgtc gcaggcggaa ctggtcatta accactaatc ctatcgcgat aatccatgca
     3961 tacgtttcgg acttttctac ttttcatatt agcatttacg ctgaatacaa cagcacaaac
     4021 gggcaccccg ctggatccta cactcgatca atcggtcaag gaaggactat tgcaaaatcc
     4081 gaatgacaaa ggcctgcaac tgatccagtc acagcagtac tcgaccgccg aaacgttctt
     4141 gaacaacagc ctgaaggaaa acgacctcga cccgaaagcc tactttcaac ggggcgtcgt
     4201 aaactggcag cagagcgata cgctggcagc ctgccgcgac tggagttcgg tgcttgcgct
     4261 gggtgacacg gctacgtaca tcctgctgac gaaaaactgc ggcggcaatg tgcacttcgg
     4321 cgacgaggtg atgccggtga agaccttccg ccgggccctg gttaccaaac ccgagccggt
     4381 gatgagcgcc gcggaagccg ataaacaaac cgaacgttat gttgatcagt taccggaatt
     4441 cccgggtggc acggaaggat tgttcggtta cctgaagaaa aacctgaaca ccgccgcggt
     4501 aaaagacggc tcggccggaa cgctggcatt cgtggaattc atcatcgacc gcaaaggcaa
     4561 agtactgtat ccgcacgtcg tacgcggcgc gaacaaggac ttgaatacgg aagcggtccg
     4621 ggtgatacgg aacatgccgg catggaaacc cgcgacgaaa aaaggacaac cggtcctggt
     4681 acgttatacg ttaccg
//
LOCUS       DAOTON010000102         4685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15045_length_4685_cov_3.612959, whole genome shotgun sequence.
ACCESSION   DAOTON010000102 DAOTON010000000
VERSION     DAOTON010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15045_length_4685_cov_3.612959"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..186
                     /locus_tag="P5292_03065"
     tRNA            115..186
                     /locus_tag="P5292_03065"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:148..150,aa:Arg,seq:cct)
     gene            202..456
                     /locus_tag="P5292_03070"
     CDS             202..456
                     /locus_tag="P5292_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRS38137.1"
                     /translation="MEYFVYAIRSLKDGRIYVGMSENVAKRLNQHNMGSTKSTKGYRP
                     WELVYSEKCVDRAKARDREKFFKSGSGKEALKRLLVFQIH"
     gene            583..2052
                     /locus_tag="P5292_03075"
     CDS             583..2052
                     /locus_tag="P5292_03075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRS38138.1"
                     /translation="MILIGAAFFRLLAVLFSKGYGMHDDHFLVIEAAQSWVDGRDYNY
                     WIPSLSPQVTEPTGHSLLYPGLHYFLFRLLEGIGMFDPQAKMYVVRLLHALLSMTIVV
                     MGYRLADRLQGKGAAKIAGLLLGMLFFMPMMSVRNLAEFVCIPALLYASVIAVREEHD
                     QRWKPLLLAGLLLGLACSVRFQTIVFSGGMGLALLILRRWRQAFALGAGFVGSLVLVQ
                     MVTDMIIWGTPFMELMAYIRYNIDNAETYGVQRWYNYILLLSGILIPPVSLFLLFGYL
                     RSWRKHLLLFLPAFAFLLFHSSFPNKQERFILPAIPFVIILGSIGWSAFHAGSQFWMR
                     NTRLYRGIWIFFWSVNLAPLLFVSTTYSHRSRVEAMVYLSRKADFRELIVEETNHDYT
                     TQPPLFYLRHWTSPLELHSGITMDTLKARMAARIPAHRPNYVVFNQEDDIDARVAAFN
                     REFPGLTYETTIYPSFVDEVMHWLNHHNANFTAYVYRIE"
     gene            complement(2378..3976)
                     /locus_tag="P5292_03080"
     CDS             complement(2378..3976)
                     /locus_tag="P5292_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRS38139.1"
                     /translation="MELEKEIAKRRTFGIISHPDAGKTTLTEKFLLFGGAIVTAGAVK
                     SNKIRKAATSDFMEIEKQRGISVATSVMGFDYQGRRVNILDTPGHKDFAEDTYRTLTA
                     VDSVILVIDCIKGVEEQTEKLMAVCRMRSTPVIIFINKMDREGKDPFDLLDELQEKLH
                     IHVRPLSWPISQGESFQGVYNLYRKELNLFEAHKTRIGESKAKISDLGDEQLDELVGK
                     KFADKLREDVQLVEGVFEPFDTGLYRDGLLAPVFFGSAINNFGVQELLETFLEIAPSP
                     RPRPTVEREVSPTEQKFSGFVFKIHANLDPNHRDRIAFLRVCSGKFERNTFYWHTRLD
                     KKVRFSNPTSFMANEKSLIEEAYPGDVVGLYDNDIFKIGDTLTEGEELHYKGIPNFSP
                     EIFRELENRDPFRTKQLEKGIQQLTEEGVAQLFVQQPGNRKIIGTVGELQFDVIKFRL
                     EHEYGAKCDFRPLNFTKAYWITADSPAELQQFMTSRANSIVYDKENRPVFLSESDWMM
                     KMIKQTFAAIHFHTSSEATTKDEE"
     gene            complement(4048..4461)
                     /locus_tag="P5292_03085"
     CDS             complement(4048..4461)
                     /locus_tag="P5292_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRS38140.1"
                     /translation="MKMSRYILVGMLLGFILYKSEAVSWYRIFEMFHFQSFHMFGIIG
                     SAIVSGIIVVQLIKREHMKNVEGQEITIKDKNKSVPRYLIGGFIFGLGWALAGACPAP
                     MFILLGSGYTVFVAYLLAAMTGTFCYGLLRKRLPH"
BASE COUNT         1023 a         1225 c         1230 g         1207 t
ORIGIN      
        1 aactttgaca gggaaaataa gtaatcaacg gccccgtagt tcaacggata gaatacctgc
       61 ctgccgtcag gcagggaagt ttctaaactt tgacagggaa aataagtaat caacggcccc
      121 gtagttcaac ggatagaata gaagtttcct aaacttttga tacaggttcg attcctgtcg
      181 gggctgcata gttttattca tatggagtat tttgtttatg cgattcgtag tttgaaggat
      241 ggtcgaatat atgttggtat gtcagagaat gttgccaagc ggttgaatca acataacatg
      301 ggatcaacaa aatctacaaa agggtatcgc ccatgggaat tggtttattc ggaaaagtgt
      361 gtggataggg cgaaagcacg tgatcgtgaa aagttcttta aaagtggctc aggtaaagag
      421 gcattgaaga gattacttgt atttcagatt cactgagaat tcgtgattga gaattacttg
      481 cctcgaagga tttggtgcca gttttattca cttttgtaga ttgcatcaaa catgacatta
      541 acgaactata tccaaaaatt atggaatagt gccccgctcg cattgatcct tatcggtgcc
      601 gcattttttc gtctgctcgc tgtcctgttc agcaaaggct atggcatgca cgacgatcat
      661 ttcctggtga tcgaggctgc gcaaagctgg gtcgatggac gagattacaa ctattggatc
      721 ccgtcactca gtccgcaggt cacggaaccg acggggcata gcctgcttta tccgggattg
      781 cactacttcc tttttcgcct gcttgaaggt atcggcatgt tcgacccgca ggcgaagatg
      841 tacgtcgtac gcttgctcca tgcgctcctg tcgatgacca tcgtggtgat gggttatcga
      901 ctggcggacc gcctgcaggg aaagggcgcg gcaaagatcg cgggcctgct gctgggcatg
      961 ttgttcttca tgccgatgat gagcgtccgc aacctggcgg agttcgtgtg cattccggcg
     1021 ctgttgtacg ccagtgtgat cgccgtgcgg gaagagcacg accaacgttg gaaacccctg
     1081 ctgctggccg gactcttact cggactcgcg tgcagcgtgc ggttccaaac gatcgttttt
     1141 tccggcggca tgggactcgc cttgttgatc ctgcgccgct ggcggcaggc gtttgcgttg
     1201 ggtgcaggct tcgtaggaag cctcgtgttg gtgcagatgg tcaccgacat gatcatctgg
     1261 ggcacgccgt tcatggagct gatggcctac atccgctaca acatcgacaa cgccgagacc
     1321 tacggcgtgc agcgctggta caattacatc ctgctgttgt ccgggatcct catcccgccg
     1381 gtcagcctgt tcctgttgtt cggctacctc cgttcctggc ggaaacatct gttgcttttc
     1441 ctccctgcat tcgcttttct cttgttccat tcgtccttcc cgaataagca agagcgcttc
     1501 atcctgcccg ccattccgtt tgtcattatc ctcggatcaa tcggttggtc ggccttccat
     1561 gccggttcgc agttctggat gcgcaacacc cgactctacc gcggcatctg gatcttcttc
     1621 tggtcggtga acctggcccc gctgctgttt gtttccacga cgtattccca tcgcagcagg
     1681 gtagaagcga tggtttatct ttccaggaaa gccgacttcc gtgagctgat cgtggaagag
     1741 accaaccacg actataccac ccagccgcct ttgttctacc tgcgacactg gaccagtccg
     1801 ctggagctgc acagcgggat cacgatggat acgctcaaag caagaatggc agcccggata
     1861 ccggcgcacc gaccgaatta cgtggtcttt aaccaggaag acgatatcga cgcgcgcgtc
     1921 gccgcgttca atcgggagtt cccgggttta acctacgaaa ccaccattta tccgagcttc
     1981 gtcgacgagg tcatgcactg gctcaatcac cacaacgcga attttacggc gtatgtatat
     2041 cgcattgagt gaacagtgaa cagtaaacag tgggtagcga atagcgaata gtgaatagcg
     2101 aatagtatgg gatgaaggtt gtttttcctc gttcctcgtc cctcatccca ccttctgtcg
     2161 gcgaactttc aaactttcaa acttccaaac tttcaaactt tcaacgaacc cggaacccgg
     2221 aacccgaaac gcggaactat tcctcgtccc tcgccccacc ttctgtcggc gaacttccaa
     2281 cttccaactt tcaactttca actttcaatt ttcaacgttt aaacgttcaa acgttcaacg
     2341 aatcctgaac cctgaaccct gaacccgaaa cgcggaacta ttcttcatcc ttcgtcgtcg
     2401 cttccgagga ggtatgaaag tggatcgccg caaaggtttg tttgatcatc ttcatcatcc
     2461 agtcgctttc cgacaggaag accggacggt tctctttgtc gtagacgatc gagttggcgc
     2521 ggctggtcat gaactgttgc agctcggccg gtgaatcggc ggtgatccag taggcctttg
     2581 tgaaattgag cggacggaaa tcgcacttgg cgccgtattc gtgctccagg cggaacttga
     2641 tcacatcaaa ctgcagttct cccaccgtgc cgatgatctt ccggttaccc ggttgctgga
     2701 cgaacaactg cgcgacgcct tcttcggtga gttgctggat gcctttttcc aattgcttgg
     2761 tgcggaacgg gtccctgttt tccaactcgc ggaagatctc gggcgaaaaa ttgggaatgc
     2821 ccttgtaatg cagttcttct ccttcggtca gggtatcgcc gatcttgaag atgtcgttgt
     2881 cgtagagtcc gaccacatcg ccgggataag cttcttcgat gagcgatttt tcgttcgcca
     2941 tgaagctggt cgggttggaa aagcggactt tcttgtcgag ccgtgtgtgc cagtagaagg
     3001 tgttgcgctc gaattttccg gagcagacac gcaggaaggc gatccggtcg cggtggttcg
     3061 gatccaggtt cgcgtggatc ttgaatacga agccgctgaa tttctgctcg gtcggggaga
     3121 cttcccgttc caccgtcggg cggggacgcg gactcggtgc gatctcgaga aacgtttcca
     3181 ggagttcctg tacgccgaag ttgttgatcg cgcttccgaa gaacacggga gcgagcagtc
     3241 catcgcgata aaggccggta tcgaagggtt cgaagacccc ttccaccagt tgcacgtctt
     3301 cccgcagttt gtcggcgaat ttttttccga ccaattcgtc gagttgttca tcgccgaggt
     3361 ccgagatctt cgccttgctt tcgccgatgc gcgtcttgtg cgcttcgaag aggttgagtt
     3421 ctttccggta gaggttgtaa accccctgga agctttcacc ctggctgatc ggccagctca
     3481 gcggccggac gtggatgtgc agcttctcct gcaattcgtc gagcaggtcg aagggatcct
     3541 tgccttcccg gtccatcttg ttgatgaaga tgatcacagg cgtactgcgc atgcggcaga
     3601 ccgccatgag tttctccgtt tgctcttcga cacctttgat acagtcgatc accaggatga
     3661 ccgaatccac ggcggtcagc gtacgatagg tgtcctcggc gaagtccttg tgaccgggcg
     3721 tatcgaggat gttgacccgc cggccctggt aatcgaagcc catcaccgag gtggcaaccg
     3781 agataccgcg ttgtttctcg atctccatga agtcggaggt agcggctttt cggatcttgt
     3841 tactcttcac cgcaccggcc gtgacgatcg cgccgccgaa gaggaggaat ttttcggtga
     3901 gggtcgtctt accggcgtcg gggtgactga tgatgccgaa agtccggcgt ttcgcgattt
     3961 ccttttcgag ctccatggaa cgggcggata aaaaagccgc gccggaattc ccggcgcggc
     4021 gcgaaagtac tcatttgccc gcaaggctca atggggtagc cgtttgcgga gcaatccgta
     4081 gcagaaggtt cccgtcatag cggccaggag gtaggccaca aagacggtgt atccgctgcc
     4141 caggaggatg aacatcgggg ccggacaagc accggcaagc gcccaaccca aaccgaagat
     4201 aaagccaccg atcagatagc gcggtacgct tttgttcttg tctttgatcg tgatttcctg
     4261 tccttcgaca ttcttcatat gctcccgctt gatcaactgc acgacaataa taccggacac
     4321 aatggcggaa ccgataatgc cgaacatatg aaagctctgg aagtggaaca tttcaaagat
     4381 ccgataccag gaaaccgctt cgctcttata aaggatgaat cccagcagca taccaaccag
     4441 gatataacgg gacattttca tggggtgagg gaatggttat ttgttatttg taattggtaa
     4501 ttagtaatta gtaattagta attagtaact agtaactaca atactattcg ctattcgcta
     4561 ttcgctattc gctattcact attcactatt cactattcac tgtttactgt ttactgttta
     4621 ctgttcacta gtcaccaacc cctatcttta ccccatgaat cgccatctcg ccaccctgtt
     4681 gctgc
//
LOCUS       DAOTON010000103         4655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15157_length_4655_cov_3.291522, whole genome shotgun sequence.
ACCESSION   DAOTON010000103 DAOTON010000000
VERSION     DAOTON010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15157_length_4655_cov_3.291522"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="P5292_03090"
     CDS             <1..557
                     /locus_tag="P5292_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRS38141.1"
                     /translation="GVAQKMEAKGGVLTEYATRTRPDRDNFPARNRIVAGLCDATLVI
                     ESAERGGALITAQFANEYNREVFAIPGRINDPYSRGCHWLIREHLARLVEQPEQFLEY
                     MGWNETADGSKLSGQSRQLPLFEELNDEERTVVESLRDSSEDLDALAFRVRMPVNRLS
                     VLLLQMEFRGIVRLRPGNRVELRS"
     gene            636..1841
                     /gene="kbl"
                     /locus_tag="P5292_03095"
     CDS             636..1841
                     /gene="kbl"
                     /locus_tag="P5292_03095"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRS38142.1"
                     /translation="MYETLQPVLRKELEEITAAGLYKQERIISTPQGAEIRANGKDVL
                     NFCANNYLGLSSHPKVIAAAKTAIDSHGYGMSSVRFICGTQDIHKTLEQKISGFLGTE
                     DTILYAAAFDANGGVFEPLFNEQDAIISDELNHASIIDGVRLCKAQRFRYKHNNMAEL
                     EQHLQATQHLRHRIIVTDGVFSMDGTIAQLDKICDLADRYKALVMIDECHASGFMGKT
                     GRGTHEHHGVMGRIDIVTGTLGKALGGAMGGFVSARKEIVDMLRQRSRPYLFSNSLAP
                     SIVGASIAVLDMLTETTDLRDKLWENTRYFRERMTAAGFDIKPGEHPIVPIMLYEAPL
                     AQQFAARLLEEGIYVIGFFYPVVAKGNARIRVQLSAGHERHHLDRAIEAFTKVGKELG
                     VLKGVNSEQ"
     gene            1955..2413
                     /gene="rnhA"
                     /locus_tag="P5292_03100"
     CDS             1955..2413
                     /gene="rnhA"
                     /locus_tag="P5292_03100"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRS38143.1"
                     /translation="MITIYTDGASRGNPGPGGYGVILMAGHHRKELSEGFRLTTNNRM
                     ELLSVIKGLEALKNAGSQVTIYSDSKYVVDAVEKGWLFGWEKKGFDKKANPDLWRRFL
                     IIYRRHKVRFVWVKGHASNPLNNRCDELAVAAALSSDLKIDHGYEESRDA"
     gene            complement(2540..3271)
                     /locus_tag="P5292_03105"
     CDS             complement(2540..3271)
                     /locus_tag="P5292_03105"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRS38144.1"
                     /translation="MARSFLLLLLLSLVVSCGKDHEFDCFKSTGAQSSEQRSLASFTR
                     IQLDDNIDLVLRLDTTSFAEVQAGENLIDGIITEVENGTLYLRNENRCNWVRNFRKPV
                     TVTVGMGQPELINYYGYGDIHCIDTIRQHDFTFNCYNGSGSIWLKLNTDRSYLQLNVG
                     RADLHAAGRSNVSFVSNNDVGHIDNRALSSDLCYLRTKSTGECRVRVERELEATVEYI
                     GDVYYSGSPYRVVVAGNGTGQVKAE"
     gene            complement(3276..4385)
                     /locus_tag="P5292_03110"
     CDS             complement(3276..4385)
                     /locus_tag="P5292_03110"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRS38145.1"
                     /translation="MRFRVALLLSLCSALAGGSPLPDSTLGSYGLEARLTYGFLVAHR
                     PMLRLLQEEHLRSFEVSLLFPSTGKKEWERGFRFPDRGLHLAIIDLGSRKLLGTGIAV
                     YPFVDFPLFFGNDWSCRLRYGMGLGYVQHTFDPEENYKNTALSSHLNGTIHFDLHVNK
                     CIGQRGAFDAGIGITHYSNGATALPNLGINLAAAHIGYRRYFGERRALHKETHDQRQL
                     RPGMTLFLSGSLKEIYPPLGPLYFASTLSGDYLLPLNPKIDAAVGADLFYDASLRVRM
                     EASEDVQQPVSTNFRPGVHGGLQFNLDPLGLIFNLGYYPYTRFKDDGRYFHRIGLRYY
                     FDRLFVCMNLKTHYARADFIEWGVGWRFRSTSPKP"
     gene            complement(4387..>4655)
                     /locus_tag="P5292_03115"
     CDS             complement(4387..>4655)
                     /locus_tag="P5292_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38146.1"
                     /translation="QVRNRVEAALLARNATGRYFRSAAVSALRSLKSTVESRTNTLDK
                     ELNQLPVTNTPQIERSRKERDRNEHRELLDRLERMLAELDATRP"
BASE COUNT         1037 a         1218 c         1384 g         1016 t
ORIGIN      
        1 tgggggttgc gcagaagatg gaagcgaagg gcggggtgtt gacggagtac gcgacccgta
       61 cccggcctga tcgcgacaac tttcctgccc gcaaccgcat cgtcgccggc ttgtgtgatg
      121 ccacgctggt gatcgagtcg gcggaacgcg gaggcgcgct cattacagcg caattcgcca
      181 acgagtacaa ccgcgaggtc ttcgcgattc cggggaggat caacgatccc tattcgcgcg
      241 gctgtcattg gctgatccgt gagcacctgg cccgcctggt cgagcaaccg gagcagttcc
      301 tggaatacat gggctggaat gaaactgcgg atggctcgaa attgtccggg cagtcgcggc
      361 agttaccgct tttcgaagag ctgaacgatg aggaacgtac cgtagtggaa tcgcttcggg
      421 attcctccga ggacctggac gcgctggcct ttcgggtgcg gatgccggtc aaccgcctgt
      481 cggtgctgct cttacagatg gaattccgcg gcatcgtccg cctgcggccc ggaaatcggg
      541 tggagttgcg gagttgattc gtggaaaagc cgtggtgatg agcttgtctg cgcgcgttaa
      601 tttttaagac ttttacgtca tcgaataact gtatcatgta cgaaacgctg caaccggtcc
      661 tccggaaaga actcgaagaa atcacggccg ccggcctcta caaacaggag cgcatcatct
      721 ccacacccca gggcgcagag atccgggcga acggaaaaga cgtactcaac ttctgtgcga
      781 acaattacct cgggctgtct tcgcatccga aggtgatcgc cgcggcgaaa accgcgatcg
      841 attcgcacgg ttacgggatg tcgtccgtcc gctttatctg tggtacgcag gatattcata
      901 aaacactcga acagaaaatt tcaggtttcc tcggcacaga ggataccatc ctctatgcgg
      961 cggccttcga cgcgaacggt ggggtattcg agccgctgtt caacgaacag gacgcgatca
     1021 tttcggacga gctgaatcat gcgtcgatca tcgacggtgt gcggctctgt aaagcgcagc
     1081 gtttccgcta caaacacaac aacatggccg agttggaaca gcacctgcag gccacgcagc
     1141 atttgcgcca tcgtatcatc gtgaccgacg gtgtgttcag catggatggg accattgccc
     1201 aactcgataa gatctgcgac ctggccgacc gttacaaggc cctggtcatg atcgatgaat
     1261 gtcacgcttc cggtttcatg ggtaagacag gccgcggtac gcatgaacac cacggtgtga
     1321 tggggcgcat cgacattgtg accggaaccc ttggcaaagc ccttggcggc gccatgggcg
     1381 gtttcgtgag cgcacgcaaa gagatcgtcg acatgctgcg gcaacgatca cgtccttatc
     1441 ttttttcgaa ttccctcgcg cctagcatcg taggagcttc catcgccgtg ctcgacatgc
     1501 tgacggagac caccgacctg cgtgataagc tgtgggagaa caccaggtat ttccgggagc
     1561 gcatgaccgc tgccggattc gacatcaagc cgggcgagca ccccatcgtg ccgatcatgc
     1621 tgtacgaagc cccgctcgcc cagcagttcg cagcgcgctt actggaagaa ggtatttacg
     1681 tgatcggttt cttttatccg gttgtcgcca agggcaacgc ccgtatccgc gtacaactct
     1741 ccgcaggcca cgaacgccac cacctcgacc gcgccattga agcgtttacg aaggtgggta
     1801 aggagttggg ggtgttgaag ggagttaata gtgaacagta aatagtgaac agtaaacagt
     1861 gaatcctgcc tgccggagct ttagcgaagg caggaaacct gaaacgccct acacttcgtc
     1921 cctcgtccct cgtccctcgt ccctcgtccc tcgcatgatc accatctaca ccgacggcgc
     1981 ttcgcgcggt aatccgggtc cgggcggata tggggtgatc ctgatggcgg gacatcatcg
     2041 gaaggaattg tcggaaggct ttcggctgac gacgaataat cgcatggagt tgttgagtgt
     2101 gatcaagggg ctggaagcgc tgaagaatgc cggcagtcag gtaaccatct actccgactc
     2161 gaagtatgtg gtcgatgccg tggagaaggg ctggctgttc ggttgggaga aaaaaggttt
     2221 tgataagaag gccaacccgg atctctggag acggtttctc atcatttatc gccggcacaa
     2281 ggtgcgcttt gtttgggtga aaggccatgc cagcaatccg ctcaacaacc gctgcgatga
     2341 actggcggtg gctgcggctt tgtcatccga tttgaaaatt gatcacggct atgaagaatc
     2401 ccgggacgcg taatgcggcc cgaagaaata gtagatagtg gtgttgcaac ttggggaagc
     2461 cggatattcc ggttatttgg gaatgtaaca tcccgccgac cgttcgaagt tgatatcaga
     2521 ttttaaaata gcggtcgagt tattccgctt tcacctggcc ggttccattc cccgctacca
     2581 ccacccggta aggacttcct gaataataaa cgtcgccgat gtactctacg gtggcttcca
     2641 gttcccgttc gacgcgtaca cggcattcac cggtcgattt tgtccggagg tagcagagat
     2701 cgctggacag ggcgcggtta tcaatatggc cgacgtcgtt gttggaaacg aaagaaacat
     2761 tgcttctgcc ggccgcgtgc aggtcggcgc gtccgacatt gagttgcaga taggagcgat
     2821 ccgtgttcag cttgagccag atgcttcccg agccgttgta gcagttgaat gtgaaatcgt
     2881 gttgccggat agtgtcgatg caatggatat cgccgtaacc atagtagttg atgagctcgg
     2941 gttgtcccat ccccacggtc accgtgacgg gcttgcggaa gttgcgcacc cagttgcagc
     3001 ggttttcgtt gcgcaggtag agcgtgccgt tttccacttc agtaatgatg ccgtcgatca
     3061 ggttttcacc ggcttgcact tccgcaaagc tagtggtgtc gagtcgtaac accaggtcga
     3121 tgttgtcgtc aagttgtatg cgcgtgaagg aagccagcga cctttgttcg ctcgactgag
     3181 ccccggtgct tttgaagcaa tcgaactcgt gatcctttcc gcaggaaacc accaggctga
     3241 gcaatagcag gagcaggaat gaacgcgcca tggactcagg gttttggtga agttgaacgg
     3301 aagcgccaac cgactcccca ttcgatgaag tcggccctgg cgtaatgcgt cttaagattc
     3361 atgcagacga ataagcggtc aaaataatag cgcaagccaa tacgatgaaa gtatcgtccg
     3421 tcatccttga atcgggtgta cggataataa ccaagattga agatcagccc gagtgggtcc
     3481 aggttgaatt gcagtccgcc gtgaacgccg ggacgaaaat tggtagagac gggctgctgt
     3541 acatcttcac tggcttccat ccgtactcgc aaggacgcgt catagaacag gtccgctcca
     3601 acagctgcgt ctattttcgg gtttagtggg agcagatagt cgcccgaaag tgtcgaggca
     3661 aaatacaagg gaccaagtgg aggatagatt tccttgagcg atccggatag gaacagggtc
     3721 atgccggggc gtagttggcg ttgatcgtgg gtttccttat gaagcgctct acgttcacca
     3781 aagtaacgtc ggtaacctat gtgcgcagcg gccaggttga taccaagatt gggcagggca
     3841 gtggcgccat ttgaatagtg tgtgatcccg ataccggcat cgaaggcgcc gcgctggccg
     3901 atgcacttgt ttacatgcag atcgaagtgg atcgttccgt tcaggtgtga actcagcgct
     3961 gtgttcttat agttctcctc cgggtcaaac gtgtgttgaa cgtaacccag tcccatgccg
     4021 taccgcaagc gacaggacca gtcgttaccg aagaagagcg ggaagtcgac aaatggataa
     4081 acggcaatac cggttccgag aagtttgcga ctgcccaagt cgatgattgc caggtgtagc
     4141 ccccggtcag gaaaacggaa tccgcgttcc cattccttct tacctgttga cgggaataga
     4201 agcgacactt cgaacgagcg aagatgttcc tcctgaagta gtcgcagcat cggccggtgg
     4261 gcgaccagga agccataggt aaggcgtgct tccaagccgt acgagccaag cgtactgtcg
     4321 ggaaggggag aaccgcccgc caatgcggaa cacagggaga ggaggagcgc tacgcggaat
     4381 cgcatctcag gggcgtgtgg catccaactc agccagcata cgttcgagcc ggtccagtaa
     4441 ttcacggtgt tcgttgcggt cgcgttcttt tcgggaccgc tcgatctgag gcgtattggt
     4501 gaccggtagt tggttgagtt ctttgtcgag cgtattcgtt ctgctttcga ccgtcgattt
     4561 caacgagcgc aaagccgata cggctgccga gcggaaataa cgcccggtcg cgttgcgcgc
     4621 gagcagtgcc gcctcgacgc ggttgcgcac ctgga
//
LOCUS       DAOTON010000104         4648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15196_length_4648_cov_3.278685, whole genome shotgun sequence.
ACCESSION   DAOTON010000104 DAOTON010000000
VERSION     DAOTON010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15196_length_4648_cov_3.278685"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1930
                     /locus_tag="P5292_03120"
     CDS             <1..1930
                     /locus_tag="P5292_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS38147.1"
                     /translation="RGGFGGRGGGRGRGGENFYNPADNFLVDQRNGISTTQSFGVNYA
                     NKWKTVDASFSYFFNRSENEAVSDLYRTYITAENSDLAYTETSNTNSINLNHRFSSRV
                     EWKLDSLNSFIIQPKFSLQHNEGDRVLLGSNISGSSITGFTDTQNGSDLDGINTSVSL
                     LWRHSFQKRGRSLSLSMTPGFNNQKGNNYLLSLLDSYNDTLAADKTDQVGRIDKQGLT
                     LNTQVNYTEPLSERSQLQVSYGGNYQRNTSDKATFNYVAATRDYIDKDSSLSNNFRTE
                     YVSHQGGLTYRYNKQKWNLGIGANYQQASLDNTFIDPEREPVARNFSSVLPNAFFQYR
                     FSQRKSLRANYRSNNNAPTADQLQSVINNSNPLQLTSGNPDLSQDFQNNLSIRYSAVN
                     TEKNSSFFALLAGSYTFDYIGNSTFVAFSDTVLSSTVTLASGSQYTRPVNLDGYYNLR
                     SFMNYSFPVKFLKSNLNLNASLNYNLTPSLINELKNEAGSGTYGAGVLLSSNISEKVD
                     FSIGTNFSYNSLHNTLQASADNEYYSQISRAKIQVMPWKGLVLLSEWNYTYNTGLSSS
                     INQNILLWNAGIGYKFGKDRAFDLRLTAFDLLEENTSVTRTTTDTYFDDSRTNILQRY
                     FMLTLTYNFKKFREAEGQ"
     gene            2181..4520
                     /locus_tag="P5292_03125"
     CDS             2181..4520
                     /locus_tag="P5292_03125"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRS38148.1"
                     /translation="MKKFTAILLVFAGFLGATDSRGQGMPDEMYLSPDGRTLYTGGQA
                     PSGLYEPYLVRRVDLQFSQSNYWTLLTQGYQNHTDLPATMIVDGVTYDSVGVRFKGQT
                     SYNTGSSQKKSFNITTNSFVSGQDLMGYQILNFNNAFQDESFLHEVIYQHQIRRHIPS
                     AKSNFIKLYINGGNWGLYPNVQQLNKEFLKEWFLTNDGSNWRADRPPGSGGGGGGGWG
                     DGTAAMNYRGPDSTDYQPYYTLKSSTKPQPWDDLVHTCDVLNNTAAGDLDSLLPAVLD
                     VDRTLWHLGSEILFSDDDSYVYKGKMDYYTFYEIETGRMTPLEYDGNSVMESMAQNWS
                     VFYNANNANYPLLNRLLNNAEWRQRYLAHFRTLIQDEMDTTTLFPLIDDYVSLIDTIV
                     QNDPKKLYSYAQFQTGVAGVKSFIRNRRSYVLSNSEVNRPVPSLSNAAWSVAGTSFGR
                     PNAGQSVDVQVEASATAGIQTVNLYYASGIVGNFAKTSMYDDGAHNDGAAGDGVWGGT
                     IPGFDAGFWVRHYFEAVAADAYNTVSYLPAGAEHDVFVYQVNLERATDTSVVINEVMA
                     INQSTITDSAGQYEDWIELYNNGSQAVDLSGYWLTDNAFNLRKWQMPAGTTILPNEYL
                     IIWADEDGGQGDYHCNFKLSASGEDLLLINPSGQLVDEVLFGQQTADQGYARVPNGTG
                     PFVIQDPTFGANNNLSTDISTLDAPVRLLVYPNPANGYVTIAGKNRNGELVEVYDMVG
                     RAVFSGRMQETLTIDTQSWTAGIYTLRCGSESRKLLVKH"
BASE COUNT         1167 a         1335 c         1149 g          997 t
ORIGIN      
        1 gcgcgggggt ttcggtggac gtggaggcgg gcgcgggcgc ggaggtgaaa acttctataa
       61 cccggctgac aatttcctcg tcgatcagcg aaacggcatc agcaccacac aatcgttcgg
      121 tgtgaactat gccaataaat ggaaaacagt cgatgccagt ttcagctact tcttcaaccg
      181 gagtgaaaac gaagcggttt cagacctcta tcgaacctac atcacggcgg agaacagtga
      241 cctggcctac acggagacct cgaacaccaa ttcgatcaac ctgaaccatc gcttcagttc
      301 gcgcgtggaa tggaaactcg actccctcaa ttccttcatc atccagccga agttctctct
      361 tcagcataat gagggggatc gggtcttgct cggaagcaac atctccggtt catcgattac
      421 cggtttcacc gacacgcaga atggctctga tctggacggg atcaacacca gcgtttcgct
      481 cctctggcgc cacagtttcc aaaaacgtgg tcgctcgcta agcctttcca tgacgccagg
      541 ctttaacaat caaaagggga ataactatct gctctcctta ctcgacagct acaacgatac
      601 gctcgcggca gacaaaaccg atcaggtcgg aagaatcgac aagcaaggtc ttaccctgaa
      661 cacacaagtg aactatactg aaccactgag cgaacgcagc caacttcaag tcagctatgg
      721 cggaaattac caacggaaca cctccgacaa agcgacgttc aactatgtgg cggccacacg
      781 cgactacatc gacaaagact cgagcctttc caacaacttc cgaacggagt atgtttcgca
      841 tcagggcgga ctgacctatc gatacaacaa acaaaagtgg aacctgggta tcggcgccaa
      901 ctatcagcag gcatcgcttg acaacacgtt cattgatccc gagcgagaac cggttgcccg
      961 caacttttcc tccgttttgc cgaacgcttt cttccagtac cgattcagtc agcgtaaaag
     1021 cctgcgggcg aattatcgct cgaacaataa tgccccgaca gccgaccaat tacaatccgt
     1081 catcaacaac agcaatcccc tgcaactcac ttccggcaac ccggacttgt cgcaggactt
     1141 tcaaaacaat ctcagcattc gctattcggc ggtgaatacc gagaagaatt cgtctttctt
     1201 cgccttgctt gcaggctcct acacgttcga ttatatcggc aacagcacct ttgtcgcgtt
     1261 ttctgacacc gttctgagca gcacggtcac acttgccagc ggatcccagt acactcgtcc
     1321 ggttaacctt gacggttact acaacctgcg ttcgttcatg aattacagct ttccggtgaa
     1381 attcctgaag tccaacctca acctgaacgc gtctcttaac tacaacctga caccgagcct
     1441 gatcaacgag ttgaaaaacg aagcgggcag cggcacctac ggcgccggcg tgttactcag
     1501 cagtaacatc agcgagaaag tggacttcag catcggcact aatttttcct acaattccct
     1561 gcacaatacc ctacaggctt ctgccgacaa cgaatactac tcacaaatca gcagggccaa
     1621 aatccaggtg atgccgtgga aagggctggt cctgctttcg gagtggaact atacgtacaa
     1681 cacggggctt agcagcagca tcaaccagaa catcctcttg tggaatgccg ggatcggata
     1741 caaattcggc aaggaccgtg cattcgactt gcgactgacg gccttcgacc tgctcgaaga
     1801 aaacaccagt gtaacccgta ccaccacgga tacctatttt gacgatagcc gaacgaacat
     1861 cctgcaacgg tatttcatgc tgacactgac ctataatttc aagaaattcc gcgaagcgga
     1921 aggacagtga ggcaggggtt gggggtttcg ggttcccggt ttcgcgtttg ttctgaacta
     1981 attcaacctg aatattccag agctcgaaac ccggaaccca aaacccggaa cttccaacca
     2041 attcaacatc ggtatattcc accatttcgt cggtcattcc tcatggtagt tcccgaaaag
     2101 gttaaacctt tgtgaacgat tgaaatccaa ggaagccgct accttagctg gaagcggttc
     2161 caccatctaa ccaaccacac atgaagaaat ttactgcgat cctgcttgtt tttgcaggat
     2221 tcctgggcgc gaccgatagc cgcggccagg gaatgccgga tgagatgtac ctgtctccgg
     2281 acgggcgaac actctatacc ggcggacagg ctcccagcgg cttgtatgaa ccctacctgg
     2341 tccgtcgggt cgatctgcaa ttctcacagt ccaactactg gacgctgctg acccagggtt
     2401 atcagaatca caccgacctg cctgcgacca tgatcgtcga tggtgtgacc tacgatagcg
     2461 tgggtgtccg cttcaaggga cagacttcct acaataccgg gagctcccaa aagaagtcat
     2521 tcaatatcac gaccaattcc ttcgtatccg gtcaggacct catggggtat cagatcctga
     2581 atttcaacaa cgcgtttcag gatgaatcct tccttcacga agtcatttac cagcaccaga
     2641 tccgtcggca tattccgtcg gccaaatcga acttcattaa actctatatc aacggcggca
     2701 actggggact ctaccccaac gtgcagcaac tgaataaaga gtttctgaaa gaatggttcc
     2761 tgaccaacga cggttccaac tggcgcgctg accgtccgcc gggatccggt ggcggcggcg
     2821 gcggtggctg gggcgatggt accgcggcca tgaactaccg cggtccggac agtaccgact
     2881 accagcctta ttataccctg aagtcatcga ccaagccgca accctgggat gacctggtgc
     2941 acacctgcga tgtgctgaac aacacggcag ccggcgacct cgattccttg ttgcctgccg
     3001 tgctggatgt ggaccgcacc ttatggcatc tcggttcgga gatcctgttc tccgacgatg
     3061 acagctacgt ctacaaggga aagatggatt actatacttt ttacgagatc gagaccggtc
     3121 gcatgacgcc gctggagtat gacggaaaca gcgtcatgga atccatggct cagaattgga
     3181 gcgtgtttta caatgccaac aacgctaatt atccattgtt gaatcgtctg ctcaacaatg
     3241 ccgagtggcg tcagcgttac ctcgcccatt tccgtaccct catccaggat gaaatggaca
     3301 cgacgacgct ctttccgttg atcgacgatt atgtaagcct gatcgacacc atcgttcaga
     3361 acgacccgaa gaaactgtac agctatgccc agttccaaac cggggtggca ggtgtcaaat
     3421 cgttcatccg aaatcgtcgc agctatgtac tcagcaattc cgaggtgaac cgcccggtgc
     3481 ccagcctgag caatgcagcc tggtcggtag ccggaacatc cttcggtcgc ccgaacgcgg
     3541 gtcagtcggt ggatgtacaa gtcgaagcat ccgctacggc cggaatccag acggtgaacc
     3601 tgtattacgc gagcggcatc gtcggcaact tcgccaagac gagcatgtat gatgacgggg
     3661 cacacaacga cggcgctgcc ggcgacggcg tttggggcgg aacgatcccg ggctttgatg
     3721 caggcttctg ggttcggcac tattttgaag cggttgcagc ggatgcttac aataccgtca
     3781 gctatctgcc tgccggagcc gaacacgatg tgttcgttta tcaggtcaac ctcgaacgtg
     3841 caacggatac gagcgtagtg atcaacgaag tgatggcgat caaccagagc accatcacgg
     3901 attccgccgg acagtacgaa gactggatcg aattgtacaa caacggttca caggctgtcg
     3961 acctgagcgg atactggctc accgacaacg cctttaacct gcgcaagtgg cagatgcctg
     4021 cgggtaccac catcctgcca aacgaatacc tcatcatctg ggccgatgaa gacggcggcc
     4081 agggtgacta ccattgcaac ttcaaacttt cggcatcagg cgaagatctg ttgctcatca
     4141 acccttccgg gcagttggtg gatgaagtgc tcttcggaca acagacagcc gatcagggat
     4201 atgcccgcgt accgaacgga accggtccgt tcgtgatcca ggatccgacc ttcggagcga
     4261 acaacaacct gagtacggac atctccacac tggatgctcc cgtgcgactg ctcgtttatc
     4321 cgaatccggc caacggctat gtcaccatcg cgggcaagaa tcgaaacggc gaactcgtgg
     4381 aggtatacga catggtcggt cgtgccgtat tcagcggacg catgcaggag acgctgacca
     4441 tcgacaccca gtcgtggacg gcgggtatct acaccctgcg ctgcggttcc gaatcgcgca
     4501 agctactggt caagcattga tcaccaccct gataaaaaaa gcccgctcag ttgagcgggc
     4561 tttttttatc actttcccgg gtagagtttc gcatgccacc tcaccatagc cttcgaaatt
     4621 tccgccggat tacccatgcg ttgttgct
//
LOCUS       DAOTON010000105         4618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15301_length_4618_cov_3.128424, whole genome shotgun sequence.
ACCESSION   DAOTON010000105 DAOTON010000000
VERSION     DAOTON010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15301_length_4618_cov_3.128424"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="P5292_03130"
     CDS             <1..332
                     /locus_tag="P5292_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38149.1"
                     /translation="THGNGVYRVSLGALPVGVDLTLQQPGFQLALMGNPVRDELLLRL
                     GLGTTGLVQFRIFDLQGRLIDQSSMQGLPRGMQLVRLPAPKTSGAYLLEVSQHGKRRV
                     VKFVSGW"
     gene            complement(333..905)
                     /locus_tag="P5292_03135"
     CDS             complement(333..905)
                     /locus_tag="P5292_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRS38150.1"
                     /translation="MSKPIRYLRKSNKNKKRVKTLATFLRGLSRRKLRGLDGLAMALH
                     KEAFKKIDCLDCAHCCKVMTPTFKPADIKRISKHLGMTYQEYQDKYLYKDETGDWMNT
                     KTPCQHLRKDNKCAIYPVRPKDCSEFPHTQNREFKAYIASTHIQNIEYCPATLHVVER
                     MHQLIIEQGKKNLSAADVKVKQAVPVGYPY"
     gene            complement(955..3429)
                     /locus_tag="P5292_03140"
     CDS             complement(955..3429)
                     /locus_tag="P5292_03140"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRS38151.1"
                     /translation="MKRSVSIYWLVIGWLLCLPAIAKGGMITGKITDEHAQPLPFVSV
                     YLEGTTQGTTSNIEGDYKLELAPGTYRLVYRMIGYRQQVEEIRLGQETVRRDIRLLPE
                     SYKLKEVTINAAAEDPAYAIIRQAQAKRSYYQDEVKAFRCESYVKSTQRLTSYPKRFF
                     GQPVDIGPLVDSASGIMYLSESVSKIAYRQPGGVKEEMLSSIVSGDPRAYSFNRGADL
                     QRINFYSALLDFGGLVPRGIVSPIAPSAMLFYRFKLEGTFVENGQLINKISVIPRRPN
                     DPVFTGDIFIVENSWRIHSADLYITRAQQMEFVDTFRVRQNYLPIGQDLWMPFNNTIN
                     FSFSFLGFVGRGQILGIFSNYQLQPEFAEKEFNNEVLKVNADANKKDSVYWAETRPVP
                     LTDDERHDYVRRDSVRQVVESKPYLDSIDRKNNKFKFNSILTSYNHSNSWKHRFWSIH
                     SPIETISFNTVEGWNGTLRATISKEKSRDDAREWKLEGNLRYGLSNTHWNPSIHFYNL
                     YNTKSQSSIHVYGGSEVLQFNRSNPITPLTNALYSLYARKNYMKIFQQQYLRLEHRSE
                     ITNGFAAGIFLEYAHREPLRNTTDYAFKLAGKERKYTSNDPLHPTSDESAFPANDLLE
                     VTLRTRFIFGQEYITRPEGKYIIGSDLPILRLQYRKAVPLDDLSPDFDEWRASLEDDM
                     RFGLLGRFEYLVAAGDFLNSKRLYLMDARHFNGNKTWFSPFRISDFMNLEYYAFSTTG
                     FYVEGHAEHHFGGFLLNKIPLIRKLKLNEVASFHYLHNDLVDQYVELGLGVEKLNLFR
                     FQVYTSIAEGKRGNFGVVVGIRKPIR"
     gene            complement(3413..4597)
                     /locus_tag="P5292_03145"
     CDS             complement(3413..4597)
                     /locus_tag="P5292_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS38152.1"
                     /translation="MKRVLLTSHKFFPEHKAGTEVLTLKIAQDLQRRGYEVKVVAACP
                     PDLDARRKVEHSFEGDFREYEYEGVPVHIVGESLRLRDYEFRSEYYHPLMKRHFQGLL
                     ADFKPDLVIVRYWLPFMGPALGTIARRLRKCGIRVIAITDNVLPHEQRPGDAALTRYF
                     VRSCDAFVAMSQSVLDDLSRFTDNPYRAFLPHPVYDIFGKTLSKSEARQRLGLPEDQR
                     LVLFFGFIRKYKGLDYLLEAMGEAELARLQVHLLVAGEFYEPEAPYLDIVKNNGALNR
                     CRFDKEFIPKERVREYFCAADLVVQPYRHATQSGITQIAYHFGRPMLVTRVGGLAEIV
                     EDGKTGYVTAPDASSIAAAIRDFYSHARESSMSEAVVAGRSRFDWSTFVMGLMKLYEK
                     IR"
BASE COUNT         1086 a         1219 c         1192 g         1121 t
ORIGIN      
        1 tcacgcacgg gaacggtgtt taccgggtca gtcttggagc tttacctgtt ggcgttgatc
       61 tgactttgca gcaaccgggc ttccagcttg cactgatggg caatccggta cgcgatgaac
      121 ttttactccg actcggcctg ggtactacag gtcttgttca attcaggata ttcgatctgc
      181 aaggtcgcct gatcgatcag tcttccatgc agggacttcc ccggggaatg caactggtca
      241 ggctgccagc cccgaaaacc agcggcgcct acctgttgga agtgtcccaa catggcaagc
      301 gccgcgtcgt gaaattcgta tcgggttggt gatcagtaag gataaccgac cggcaccgct
      361 tgctttactt taacgtcggc agcggagaga ttcttctttc cctgctcgat gatgagctga
      421 tgcattcgct ctaccacatg tagtgttgcc ggacagtatt cgatattctg aatgtgcgtg
      481 gaggcgatat aggccttgaa ttcccggttc tgggtatgcg gaaattcact gcaatccttc
      541 ggacgcaccg gatagatggc acacttgtta tccttgcgaa ggtgttggca aggggttttg
      601 gtgttcatcc agtccccggt ctcgtccttg tagagatact tatcctggta ctcctggtag
      661 gtcataccca ggtgcttgga gatgcgcttg atgtcggcag gtttgaaggt cggcgtcatg
      721 accttgcagc aatgcgcgca atccaggcag tcgatcttct tgaaagcctc cttatgcagg
      781 gccatggcca gaccatcaag cccgcgcagc tttcttcgtg ataaaccgcg tagaaaggtt
      841 gcgagtgttt ttacacgctt cttgttctta ttgctcttac gtagataccg gatgggtttt
      901 gacatataca agctattgcg gcgcaaagaa aaggaatttc acgggacgac caagttaccg
      961 gatgggcttt cggatgccga ccacgacacc aaaatttccc cgcttccctt ccgcgatgga
     1021 cgtataaacc tggaagcgga acaggttgag cttttccacg cccagcccca gttctacata
     1081 ttgatcaaca aggtcgttgt gcaggtaatg gaaactggca acttcgttga gcttcagctt
     1141 tcggatcaag gggatcttat tcagcagaaa gcctccgaaa tggtgctcgg catggccttc
     1201 aacgtaaaat ccggtggtac tgaaggcata gtactccagg ttcatgaaat cgctgatccg
     1261 gaatggagaa aaccaggttt tgtttccgtt gaaatgccgg gcgtccatca gatagaggcg
     1321 cttgctgttt aaaaagtctc cggccgccac gaggtattca aatcgaccca acaagccgaa
     1381 tcgcatatcg tcttccaggc tggcacgcca ttcgtcgaag tcgggactca agtcatccag
     1441 cggtaccgcc ttacgatatt ggaggcgcag gattggcagg tcggaaccga tgatatactt
     1501 accctccggt cgcgtaatgt actcttgacc gaaaatgaac cgggtccgga gcgtcacttc
     1561 caacaggtcg ttcgcgggaa aagcgctttc atccgaagta ggatggagcg gatcattgga
     1621 cgtgtacttt ctttccttac cggccaactt gaacgcgtaa tcggtggtgt tccgcaaggg
     1681 ctcccggtgc gcatactcca ggaatattcc ggctgcaaag ccgttcgtga tttcagaacg
     1741 atgctccaaa cgcaggtatt gctgctggaa gatcttcatg taattcttcc gggcatagag
     1801 cgaatacagg gcgttggtca gcggcgtgat cgggttgctt cgattgaact gcaacacttc
     1861 acttcctcca taaacatgga tactggattg tgatttggta ttatagagat tgtagaaatg
     1921 aattgacggg ttccaatgcg tattggaaag tccatagcgg agattccctt ccagcttcca
     1981 ctcccgggca tcgtctctac ttttctcctt actgatcgtc gcgcgcagtg ttccattcca
     2041 accttccacg gtattgaatg aaatcgtctc gataggcgaa tgaatggacc agaagcggtg
     2101 cttccacgaa ttgctatggt tatacgaagt cagaatagaa ttgaatttga atttgttgtt
     2161 cttccgatca atggaatcga gatagggttt tgattccacc acctgccgga cactgtcgcg
     2221 ccgtacataa tcgtgccgct cgtcatccgt cagcggaaca gggcgcgttt cagcccaata
     2281 aaccgaatcc ttcttgttag catcggcatt gactttcagg acttcgttgt tgaactcctt
     2341 ctccgcaaac tccggttgga gctggtaatt ggaaaagata ccgaggatct gtccgcgccc
     2401 cacaaagccc agaaaggaaa aggagaaatt gatggtattg ttaaacggca tccacaggtc
     2461 ttgtccaatg ggtaaatagt tctgcctgac ccggaatgta tccacgaact ccatctgctg
     2521 cgccctggtg atgtacaggt cggcagaatg aatacgccaa ctgttctcca caatgaaaat
     2581 gtctccggta aatacaggat cgttcggcct gcgtgggatg acactgatct tgttgatcag
     2641 ttgcccgttc tccacgaatg tgccttccag tttgaagcga tagaagagca tcgccgaagg
     2701 ggcgatcggc gacacgattc cgcgcgggac caggcccccg aaatccagca aggccgagta
     2761 aaaattgatc cgctgtaaat ccgcgccccg gttgaaactg tacgcgcgcg ggtcaccact
     2821 cacgatactc gacaacatct cctccttgac tccgccgggc tgccgatagg cgatcttcga
     2881 taccgactcg ctgaggtaca tgataccgct ggcggaatcc accagtggac cgatatccac
     2941 cggctgtcca aagaatcttt tcggataaga tgtcagccgc tgtgtcgatt tcacataact
     3001 ctcacaacga aatgccttga cttcatcctg atagtacgac cgcttcgcct gcgcttgccg
     3061 gatgatggca tacgcggggt cttccgcagc ggcattgatc gtgacctcct tgagcttata
     3121 ggattccggc aagagccgga tatccctgcg cacggtctcc tgtcccaaac ggatctcctc
     3181 tacctgttgc cggtaaccga tcatgcggta aaccagacga tatgtaccgg gggcaagttc
     3241 gagtttatag tccccttcga tgttggaggt ggtcccttgg gttgttcctt ccaggtaaac
     3301 actgacgaac ggcaacggct gcgcgtgctc atcggtgatc ttaccggtga tcattccacc
     3361 cttcgcgatt gcaggcaaac aaagtaacca gccaatgact aaccaataaa tgctaacgga
     3421 tcttttcata caatttcatc aatcccataa caaacgtgct ccagtcaaac cgacttcgtc
     3481 cggcaacgac ggcctccgac atgcttgatt cacgtgcatg cgaataaaaa tcacgaatgg
     3541 cggcagcaat ggacgaagca tcgggagccg taacataacc tgtttttccg tcttcaacga
     3601 tttcagccaa accacccacc cgggtaacca gcatgggacg gccgaaatga taagcaattt
     3661 gtgtaatacc gctttgggtt gcatggcgat agggttgaac aaccaaatcg gccgcacaga
     3721 aatactcccg tacccgctcc ttggggatga actccttgtc gaaccggcat cggttgagcg
     3781 cgccattatt ctttacgatg tcgagatagg gagcttccgg ctcataaaac tccccggcaa
     3841 ccaacagatg aacctgcagc ctggcgagtt cagcttcccc catggcctcc agcaggtagt
     3901 caaggccttt gtattttcgg atgaagccga agaacaacac caggcgttgg tcttccggta
     3961 agcccaaccg ctgacgggct tcggatttcg aaagcgtctt gccgaagatg tcgtagaccg
     4021 gatgcggaag aaaggcccgg tagggattgt ccgtgaaccg gctgaggtcg tccaataccg
     4081 actgtgacat ggcaacgaaa gcgtcgcagg aacggacgaa atacctggtc aaagctgcat
     4141 cgcccggtcg ttgttcgtgc ggcagtacat tatcggtaat ggcgatcaca cggatgccgc
     4201 atttgcgcaa cctgcgggcg atggttccca aagcggggcc catgaacggc aaccagtaac
     4261 ggacgatgac caggtcgggt ttgaagtcgg ccagtagacc ctgaaaatgc cgcttcatga
     4321 gcggatgata atactctgag cgaaactcgt aatccctcag cctcagggac tctcccacta
     4381 tgtggaccgg cacgccctca tattcatatt ctcgaaagtc accttcaaaa ctatgctcga
     4441 ccttgcgcct ggcatcgagg tcaggcgggc aggctgccac caccttcact tcatagcccc
     4501 gtctttgcag gtcctgggca atcttgagtg taagtacttc cgtaccggct ttgtgttcag
     4561 gaaaaaactt atgggaggta agtaaaactc ttttcatgcg accttttatg ttaccagg
//
LOCUS       DAOTON010000106         4609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15330_length_4609_cov_3.741326, whole genome shotgun sequence.
ACCESSION   DAOTON010000106 DAOTON010000000
VERSION     DAOTON010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15330_length_4609_cov_3.741326"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="P5292_03150"
     CDS             <1..995
                     /locus_tag="P5292_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38153.1"
                     /translation="VVIDSLYELPAIPLVTASRANPICPGDSVQLFIYSGSGTQAWYQ
                     FDGSVIDFIVGATDTSLFAFENQGYGVRVTDNNSCSVLSDPYWVELDQELYFVNANGP
                     ISFCAGGQVQLTAGAGSEYAWSSLDSVQTITVSTTGLYSYTCISPAGCPKSSDTLQVT
                     ILDLPVINLGADTVLCTADTLMLDAGSGFLFYQWSDGSSDQYFSLPFTSPDDTLYIAA
                     YVTDTNYCSNGDTVRVIMTICDGINSTALPEVLQVFPNPVPTGWDAVRVEFADADVIG
                     GLLSLFSQDGKWILDLEMVAPPGLIRLPRLSTGVYYLVFSKNGRISATRRLTVQ"
     gene            1045..1464
                     /locus_tag="P5292_03155"
     CDS             1045..1464
                     /locus_tag="P5292_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38154.1"
                     /translation="MRRILAIGLASIIFLITSGMIVTVHECHSEGKKDLSVFQDKGCC
                     SKKTNSCKSGSSDRMKAIECCDVTTTWIAAHVETILLDSKTDLSPKAFFSAFFASFSP
                     SLANPLAVFSVNSISDPPDLDKSYLTFGLTDLGVFRI"
     gene            1533..3794
                     /locus_tag="P5292_03160"
     CDS             1533..3794
                     /locus_tag="P5292_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS38155.1"
                     /translation="MNTNIIQQLLRSCRRILTSIVLLLCSATVYGQSGNLRGTVRSTA
                     SSAGQTPAPLPGATVLWLGTIDATTTDANGNFEIRPPAGFPARLVVSYVGFLSDTVNV
                     EQSQFLEISLKSSAQLKEVEVTGKQDAISISSLSTIQVEKINQKELLRAACCNLSEAF
                     ETNPTVSVAYTDAVTGAREVQLLGLSGIYSQLTIENIPAFRGLAAPYGLTFVPGPWME
                     SIQVTKGAGAIVNGFEGTSGQINIEYIKPDNDDQPRFFLNAFAEPMGYSDLNAFRRFR
                     LNDHWSTLLMVHGDKMFGSQDRNKDGWMDIPMGQQVNLYNRWQYHSRKKLEAQFGFRF
                     MLDDRDGGQVAGEGSGNHHGGDYMTSVQNARTEVFGKLGIVFPEKPLKSIGNIAQVSL
                     HRLRSSFGFRSYNADQTSFFVQSIYQNAINAGQHQYKIGLNLRYDLLEESLSGSPDTL
                     EELVPGGFLEYSWHYFDQLTIVAGYRLDYHNLFGVFSTPRVHLKYDISEDLVLRASAG
                     KSYRVPYRIAENLSLLVSSKRFDFQDELRPEQAWSYGFNLLKRYRLFGKDGSVLLDLF
                     HTDFIDQLVVDRYADSSRVVFFNLKGRSYSNSAQFTVNQEILSQLEIRLALKIDDVRT
                     DYLNGLARKPLVPYQRALGTLHYESTDRRWKADYTMVWTGMKALPVTFTDPVTGIQDK
                     ESPSFFTTNIQLTRAFRRLELYAGSENLFDFRQPAPIINRDDPYSDRFDAANVWGPIL
                     GRRIYAGFRFKWL"
     gene            3849..4199
                     /locus_tag="P5292_03165"
     CDS             3849..4199
                     /locus_tag="P5292_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019990205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRS38156.1"
                     /translation="MYKFTLAAFLLLLVITAETVAQQTSDTVSVLTTAICSSCKNRIE
                     NDLSFEAGVRKVTMDLDTKRVSVVYNPQKTDPDKIRLAITRIGYDADSLKADQKAYKR
                     LPECCRNHDAEHPH"
     gene            complement(4200..>4609)
                     /locus_tag="P5292_03170"
     CDS             complement(4200..>4609)
                     /locus_tag="P5292_03170"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS38157.1"
                     /translation="WAEERLGKDRMTGAYAYRSLEREAETIAFGTDFPVERINPLLTF
                     YAAVSRKDLEGNPPDGFRAEEAMKRKDVLKAMTTWAAYANFEEEEKGLIDNGYFADFI
                     VLDRNIMEIDEEQIPQTRVLSTYVNGELVYGAE"
BASE COUNT         1068 a         1192 c         1163 g         1186 t
ORIGIN      
        1 gcgtcgttat cgactcgctc tacgaactgc cagccattcc gttggttacc gcgagccggg
       61 ccaatccgat ctgtccgggc gacagtgtcc agctatttat ttattccgga agcggaacgc
      121 aagcgtggta tcaattcgac ggaagcgtaa ttgactttat cgtcggtgcg acggatacca
      181 gcttattcgc ctttgaaaat cagggatacg gagttcgcgt aaccgacaac aattcctgta
      241 gtgttctgtc ggacccctac tgggtcgagc tggatcaaga gctttacttc gtgaatgcca
      301 atggtccgat tagtttttgt gccggtggcc aggtgcagtt gacggctggg gccgggagtg
      361 aatatgcatg gtcgtccctg gattcggttc agacaataac cgtcagtacc actggtctgt
      421 acagttacac ctgcatcagt ccggccggat gtccgaaaag ttccgatacc ctgcaggtca
      481 ctatcctcga cctgccggtg atcaacctgg gcgccgatac ggtattatgt accgctgaca
      541 cgctgatgct cgatgcggga agcggcttcc tgttttatca atggtcggat ggatcgtcgg
      601 accagtattt ctcgttgcca ttcacttcac cggacgatac cctttacatc gccgcttacg
      661 tcaccgatac gaactattgt tcaaacggag acaccgtacg cgtgatcatg accatctgtg
      721 acggaatcaa tagcacggcg ttgccggaag ttttacaagt cttccccaac cctgttccga
      781 cgggatggga tgcggtccgt gtcgaattcg ctgacgctga tgtaataggc ggactactga
      841 gtctcttcag tcaggacggc aaatggatcc tcgatttgga aatggttgcc ccgcccgggt
      901 taatcagatt gccacgtctg agtaccgggg tgtattacct tgttttttca aagaatggga
      961 ggatttcggc aacccgccgg ctaaccgttc agtagtataa gttcttcatc tttcgataat
     1021 tccggttttc tacctttgca aaacatgcga cggattctcg ccatcggatt ggcctctatt
     1081 atcttcctga tcacttcagg aatgatagtg accgttcatg aatgccattc ggaaggcaaa
     1141 aaagatctgt ccgtttttca ggataaaggc tgctgcagta aaaaaacgaa ttcgtgtaaa
     1201 tccggatcat cggatcgcat gaaggcgatc gaatgttgcg atgtcacgac cacctggatc
     1261 gcagcacatg tcgagacgat tttacttgat tccaagactg acttatcacc caaagcgttt
     1321 ttttccgcgt tttttgcaag tttttcgcct tctttagcca accctttggc tgttttttca
     1381 gtaaattcaa tatcggatcc tccggatctt gataagtcct acctgacatt cgggttgact
     1441 gatttgggtg tcttcaggat ttgatttgcc gttcgtgcaa tcggtttccg tttggtaaac
     1501 ggaagttgtt tcatccttaa cggcattcat ccatgaatac gaatatcatc cagcaactac
     1561 tgcgttcttg tcggcggatt ctgacaagca tagtcctgct actttgctcc gccactgttt
     1621 acggccagtc tggcaatctt cgtggtacgg taagaagtac ggcctcgtcc gctggtcaga
     1681 cgccagcacc acttccggga gctactgttt tatggctcgg taccatagac gctaccacga
     1741 ccgacgccaa tggaaatttc gaaatccgtc cgcctgccgg gttccctgcc cggttagtcg
     1801 tctcctatgt tgggttctta tcggataccg tgaacgtaga gcaatcgcaa ttcctggaaa
     1861 tcagcctgaa atcttccgcc caattgaagg aagtggaagt gaccggcaag caggatgcga
     1921 tcagcatttc cagtctcagt accattcagg ttgaaaagat caaccagaaa gaattactgc
     1981 gggccgcctg ttgcaatctc tcggaagcct ttgaaaccaa tcctaccgta agtgttgcct
     2041 atacggatgc ggtcactgga gcccgagaag tgcaattgct cggactttcc ggtatctaca
     2101 gccagcttac gatagaaaac attcccgcgt tcagaggact tgccgctccc tacgggctca
     2161 cctttgttcc cggcccctgg atggagtcca tccaggtaac gaaaggagcc ggtgctatcg
     2221 tcaacggatt cgaggggact tcgggtcaga tcaacatcga gtacatcaaa cccgacaacg
     2281 acgatcagcc acgttttttc ctcaacgcat ttgctgagcc gatggggtac tcggacctca
     2341 atgcctttcg tcggttccgg ttgaatgacc attggagcac gctcctgatg gttcatggag
     2401 acaagatgtt cggaagtcag gaccgcaaca aagacggctg gatggatatt cccatgggtc
     2461 agcaggtcaa tctttacaat cgctggcaat atcatagtag aaaaaagttg gaagcccagt
     2521 tcggattccg gttcatgctt gatgatcgcg atggcggaca agtggcaggg gaagggagtg
     2581 ggaatcacca tggcggagac tatatgactt ctgtgcagaa tgcccgaacg gaagtattcg
     2641 gaaaactcgg gatcgtcttt cctgaaaaac ccctgaagag catcggcaat attgcccaag
     2701 tcagccttca tcggctgcgc tcttcattcg gcttccgttc ctacaatgcg gatcaaacca
     2761 gctttttcgt tcagtcgatc taccaaaatg cgattaatgc cgggcagcat cagtacaaga
     2821 tcggtctgaa cctgcgctac gacttgctgg aagaatccct gtcaggtagc ccggatacac
     2881 tggaggagct agtcccgggc ggatttctcg aatactcctg gcattatttc gaccaactga
     2941 cgatcgtagc gggataccga ctggattacc acaatctgtt tggcgttttt tcgacgccca
     3001 gggttcattt gaaatacgat atcagcgaag acctggtcct ccgggcgagt gccggaaaaa
     3061 gctaccgggt accataccgg atcgccgaaa acctgtcgtt gctggtatcc agcaagcggt
     3121 ttgattttca ggatgagctt cgtccggagc aggcctggag ctatggattt aacctcctaa
     3181 aacgataccg gctctttggc aaggatggtt cagtcctgct ggatctgttt cataccgatt
     3241 ttatcgacca gcttgttgtg gaccgatatg cagactcttc ccgcgtggtg tttttcaacc
     3301 tgaagggaag gagctactcg aacagtgcgc agtttactgt caaccaggag atcctgtcac
     3361 agttggagat ccgactggcg ctcaagatcg atgatgtccg taccgattac ctgaacggct
     3421 tggcccggaa accactggtt ccgtatcagc gtgccttggg tacacttcac tatgaatcga
     3481 cggatcggcg ctggaaggcc gactatacca tggtctggac tggtatgaaa gcgctaccgg
     3541 tgacgttcac cgatccggtg acgggaatcc aggacaagga atcaccttcc ttcttcacga
     3601 ccaatatcca attgacccgc gcttttcggc gattggaatt gtatgccggt tctgaaaacc
     3661 tgttcgactt ccgccaaccc gctccgatca tcaatcgcga tgacccttat tccgatcgat
     3721 ttgatgcagc gaatgtctgg gggccgattc ttgggcggag gatctacgcc ggattccggt
     3781 ttaaatggct ctgatgaccc ggcattaggg tataattctt gatactgttg atatataaaa
     3841 accttcccat gtataagttc acgctggctg cgttcctgct tcttctcgtc ataaccgccg
     3901 aaacggttgc ccaacaaaca tccgataccg tcagcgtttt gacgacggcg atctgttcat
     3961 cgtgcaagaa tcgaatagaa aacgacctga gcttcgaagc cggagttcga aaagtaacca
     4021 tggaccttga cacgaagcgg gtgagtgtcg tttacaaccc ccaaaagacg gacccggata
     4081 agatccggct ggccatcacc cgcatcggtt atgacgctga ttccttgaaa gcggatcaga
     4141 aagcgtacaa acgacttccg gaatgttgcc gtaatcacga tgcggaacat ccgcactgat
     4201 cattcagctc cataaaccag ttccccgttt acatacgtac tcagtacacg ggtctgtggt
     4261 atctgctctt cgtcgatctc catgatgtta cggtcaagca cgatgaagtc cgcaaagtaa
     4321 ccgttgtcga tcaagccttt ttcctcctct tcaaaattag cataggccgc ccaggtggtc
     4381 atggctttca ggacatcttt acgcttcatc gcttcttccg ctcgaaaacc atccggtgga
     4441 ttgccttcaa ggtcctttcg tgacacggcg gcataaaagg tgagaagggg gttgatgcgt
     4501 tcgacgggaa agtcggttcc aaaagcgatg gtttcagctt cccgctcgag tgagcgataa
     4561 gcgtacgctc cggtcattcg gtctttgccc aaacgttctt cggcccagt
//
LOCUS       DAOTON010000107         4605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15346_length_4605_cov_3.649670, whole genome shotgun sequence.
ACCESSION   DAOTON010000107 DAOTON010000000
VERSION     DAOTON010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15346_length_4605_cov_3.649670"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="P5292_03175"
     CDS             <1..714
                     /locus_tag="P5292_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS38158.1"
                     /translation="AYRKAQELGSKEFTVISQILFLDSQLQDWDAMIRDSEEAMNLFP
                     DQPLVYFFNGIANSQKKQFAKAVVSLNAGVKLVVDNKELESNFYASLGDAYQELKDFT
                     RSDENYDKALDIDPKNANVLNNYAYYLSVRGERLEKAEQMSKLSNELEPNQASYEDTY
                     GWIMFRLGRYNDAKEWIGKSLKNSNDNSATVLEHYGDVLFKLGDSAGALDYWQRARNA
                     GEGASEFLEKKILERKYIE"
     gene            810..1643
                     /locus_tag="P5292_03180"
     CDS             810..1643
                     /locus_tag="P5292_03180"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HRS38159.1"
                     /translation="MNSTRFGIALGILISTLVLFSSCKTSKKIVESVPVPVKVKGDDA
                     AAVFDSVSARAFRSEWLTAKAQVDYTDKRGETNSFDVNMRLRKDSLLWISITPLLGIE
                     AARVLITPDSIVILDRVHKEVAIRKYDYLEEMLHAPVSFAMIQAVIAGNYFPYRTNEK
                     IRSLYEEEPYFILSTLNKRQARRAMEEKDPNTPVVQDFWIDGNYRIAKAKITDDKLDR
                     WVEARYTAFDSVSTGLFPQQVIVTVSGSTPVIMKITYDKITPDEPVAMPFTVPEKYER
                     K"
     gene            2172..3446
                     /locus_tag="P5292_03185"
     CDS             2172..3446
                     /locus_tag="P5292_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRS38160.1"
                     /translation="MSLVRTLGLVGICLLVLLSGAFAQSKKELERKKAQLHKDIEYTN
                     QLLKETKKNKSSSLNQLVNLNRKINRRTELIQTIQSEIGSVDKNIGTVETNIDSLNQR
                     LARLKDQYARMLYYAYKNQRPASQLLFVFSAENFNQAFKRMQYLRELSRYRHRQHDMI
                     VAIQDSLSGKKRQLQDVRKEKSGLLVAKEQEKKELDKEKKEQVSLLSNLSAKEKKLRA
                     DLREKQRKEQQLSARIESIIRKEIEAAQAAARKRSSSGATASKSNNVNRTTEKYNSPS
                     VLSNTPEAAKLSSDFENNRGRLPWPVEQGVITSSFGRHPHPVWRDVVVNNNGVDIGSA
                     RGARARSVFEGKVLRVIMVVDKYAVLVQHGEYFTLYSNLSEVFVKAGDKLITRQPIGR
                     IQTSDEDGRTEVHLEIWKGSTKMDPEGWLASR"
     gene            3568..3756
                     /gene="tatA"
                     /locus_tag="P5292_03190"
     CDS             3568..3756
                     /gene="tatA"
                     /locus_tag="P5292_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRS38161.1"
                     /translation="MLASILLGFLGGLGGGEIFIILVILLLFFGAKRIPELAKGLGKG
                     IKEFKDAKNGSDSKEDAK"
     gene            complement(3762..4019)
                     /locus_tag="P5292_03195"
     CDS             complement(3762..4019)
                     /locus_tag="P5292_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38162.1"
                     /translation="MGYDDITPHLNKIATLYLKGNKRKVGWIFVDRKQANSTPPAEIS
                     FISVQKGKRLLEALENRETEALEPFREVLPVMLIERIRSSK"
     gene            complement(4006..4335)
                     /locus_tag="P5292_03200"
     CDS             complement(4006..4335)
                     /locus_tag="P5292_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38163.1"
                     /translation="METALLGCALPVVAHLLEDFLRSRGYQIESDKDHPEHILAYQDG
                     SWFRSPRRVEINLLPVGEKTTRVEVTAEIESRKRNEEAEEILEEKIIEAICKNYQTTL
                     QQNNGLR"
BASE COUNT         1067 a         1303 c         1289 g          946 t
ORIGIN      
        1 gcctaccgca aggcacagga actcggctcg aaggagttca ccgtcatcag ccagatcctc
       61 ttcctcgact cgcagttgca ggattgggac gccatgatcc gtgacagcga ggaggcgatg
      121 aacctgttcc ccgaccagcc gctggtgtac ttcttcaacg gcatcgccaa cagccagaag
      181 aaacagttcg ccaaagcggt ggtttcgctc aatgccggcg tcaagctggt ggtcgataac
      241 aaggagctcg aatcgaattt ctacgccagc ctcggcgacg cctaccagga gctgaaggac
      301 ttcacgcgca gcgacgagaa ctacgacaag gcgctggata tcgatccgaa gaacgccaac
      361 gtgctgaaca actacgccta ttacttgtcc gtccgcggcg agcggctcga aaaggcggag
      421 cagatgtcga agctttcgaa cgagctcgaa cccaaccagg cttcctacga agacacctac
      481 ggctggatca tgttccgcct cggcaggtac aacgacgcca aagaatggat cggcaagtcg
      541 ctgaagaaca gtaacgacaa cagcgccacg gtgctcgagc actatggcga tgtattgttc
      601 aagctgggcg acagcgccgg cgccctcgac tactggcagc gcgccaggaa tgccggcgaa
      661 ggcgcttccg agtttttgga gaagaagatc ctggaacgga agtacatcga gtaagctgcc
      721 gcttcccttg ctgtttatcg tgtacctgaa ccccggcccc gcgcgcctgc cttgatttcg
      781 tgcaggatgc ctacattcgt taccggccca tgaactccac acgtttcggt atagcgctcg
      841 gcattctgat cagcacgctt gtgctttttt cgtcctgcaa aaccagcaag aagatcgtcg
      901 agtccgtgcc ggtcccggtg aaggtgaaag gcgatgatgc cgccgcggta ttcgacagcg
      961 tctcggcccg tgctttccgc agcgaatggc ttaccgccaa agcgcaggtg gattataccg
     1021 acaaacgcgg cgagaccaat tcgttcgacg tgaacatgcg gctccgcaag gacagcctgc
     1081 tctggatctc catcaccccg ctgctgggca tcgaagcggc ccgcgtactc atcacgcccg
     1141 attcgatcgt catcctcgac cgcgtacaca aggaagtggc gatccgcaag tacgactacc
     1201 tcgaggaaat gctgcacgcg cccgtcagct tcgcgatgat ccaggcggtg atcgccggca
     1261 actactttcc ttaccgtacc aacgaaaaga tcagatccct ctacgaggaa gaaccctact
     1321 tcatcctcag caccctcaac aagcgccagg cccgacgcgc gatggaggag aaggatccca
     1381 acacgccggt cgtgcaggat ttctggatcg acggcaacta ccgcatcgcc aaagctaaga
     1441 tcaccgacga caaactcgac cgctgggtcg aagcacgcta taccgccttc gactccgtct
     1501 ccaccggcct cttcccgcaa caagtcatcg tcacggtctc cggctccaca ccggtcatca
     1561 tgaagatcac ctacgacaaa atcacccccg acgaacccgt cgccatgccg tttaccgtgc
     1621 cggagaagta cgaacgcaag tgagtttatt gttccgggtt ccgggttagg aagtagtaga
     1681 tggacattaa acactcttca aaacagtaca aaactcggaa cccggaactc ggaacccgga
     1741 actcccccgc accttcccca ggcaattcgc aaaggatcca tcatgatcat taggagctga
     1801 acgaacgtgc attcctcacc atcggtcccg tgttcgctcg tcacctaagc gccgatccgt
     1861 ttctcaacga actgctcgac gagctcaccc ggggctaggt tgcatggttg gtagcttgta
     1921 tcagttgttt gttgtttcgg gtttccagtt tcctgtttcg ggtttccggt tttgggatga
     1981 gtaattcctt atttcataag tcctgaagac agttgcattc caacaactcg aaacccgaaa
     2041 ctcggaaccc ggaaccgtaa cattcccgaa cattttcccc atttccccgt tcaacttcct
     2101 tgctgaattc tccgttatct tagggttggc aagcagtaga aatgtccttt tttgttgaaa
     2161 ataagcaagt tgtgagtctt gtccggacgc tcggactggt aggcatttgc ctgctggtgc
     2221 tgctttccgg cgcgtttgcg cagagtaaga aagagctgga acgcaagaaa gcgcaactgc
     2281 acaaggacat cgagtacacc aaccaactgc tcaaagagac caagaagaac aagagttcgt
     2341 ccctcaatca attggtcaac ctgaaccgga agatcaaccg gcgcacggaa ttgatccaga
     2401 cgatccagtc ggagatcggt tcggtcgata agaacatcgg tacggtggag acgaacatcg
     2461 attcgctcaa ccagcgtctt gcaagactga aagaccagta tgcgcggatg ctgtactacg
     2521 cgtacaagaa ccagcgcccg gcctcgcaac tgctgttcgt gttctcggcg gagaacttca
     2581 accaggcgtt caagcgcatg cagtacctgc gtgagctgag ccgctaccgc catcggcagc
     2641 acgacatgat cgtcgcgatc caggattcgc tcagcggcaa gaagcgtcag ttgcaggatg
     2701 tacgcaaaga gaagagcgga ctgctcgtgg cgaaggagca ggagaagaaa gagctcgaca
     2761 aggagaaaaa ggaacaggtt tcgctgctga gtaacctcag cgcgaaggaa aagaaattgc
     2821 gcgccgactt gcgcgagaag caacgcaagg aacagcagct ttcggcccgc atcgaatcga
     2881 tcatccggaa ggagatcgaa gcggcccagg ctgcggcgag aaaacgttcg tcgtcgggcg
     2941 ctaccgccag caagagcaac aacgtcaacc gtacgacgga aaaatataat tcaccttccg
     3001 tgctctccaa tacgccggaa gcggcgaagc tgagcagcga tttcgagaac aaccgcggtc
     3061 gtttgccctg gccggtcgag cagggcgtga tcaccagttc ctttggtcgt catccccacc
     3121 cggtctggcg cgatgtggtg gtgaacaaca acggcgtcga catcgggtcc gcacgcggcg
     3181 cgcgcgcgcg ttcggtgttc gaaggaaagg tactgcgcgt gatcatggtg gtcgacaagt
     3241 acgccgtgct cgtgcagcat ggcgagtact tcaccttgta ttcgaatctc tccgaagtct
     3301 tcgtgaaagc aggggataaa ctgatcaccc ggcaaccgat cgggcggatc cagaccagcg
     3361 acgaagacgg gcgtacggaa gtgcacctgg agatctggaa aggcagtacg aagatggatc
     3421 ccgaaggctg gctggcttcc cggtgatctt cggaaactgc tcaattcgcc tgaaaagggc
     3481 gaaatcgatc gtaagcggga cggttcagcc ggaattcacc cgccggcggt cgggaattga
     3541 ttacttttgc aacatattca ttcagcgatg ttagcatcca ttctgctcgg attcctcgga
     3601 ggccttggtg gcggcgagat tttcatcatc ctcgtcatcc tcctcctgtt cttcggcgcc
     3661 aagcgcattc cggaacttgc gaagggtctc ggcaagggca tcaaggagtt caaagacgcc
     3721 aagaacggct ccgactccaa agaagacgcc aagtaaaacc cttacttgct cgaacggata
     3781 cgttcgatca gcatgacggg aagcacttcc cggaagggct cgagcgcttc ggtttcccgg
     3841 ttttccaacg cctccaggag gcgttttcct ttttgtacac tgatgaagga gatctccgca
     3901 ggcggtgtgg agttcgcttg tttgcggtcg acaaagatcc agccgacctt tcgcttgttg
     3961 cctttcaggt acagcgtggc gatcttgttc agatgcgggg tgatgtcatc gtagcccatt
     4021 gttctgttgt agggtagttt ggtagttttt acaaatggcc tcgatgatct tctcttccag
     4081 gatctcttcg gcttcctcgt tacgtttgcg tgattcgatt tcggcggtga cttccacccg
     4141 ggtggtcttc tccccgacgg gcaggaggtt gatctccacc cgtcggggac tcctgaacca
     4201 ggaaccatcc tggtaagcca ggatgtgttc aggatggtcc ttgtcggact cgatttgata
     4261 gcccctgctg cggaggaagt cctcgagcag gtgagccact accggtagtg cgcagccaag
     4321 cagcgccgtt tccattgcca tgggcaatac attttcagcc gcggttaagc gggcgttatt
     4381 cccagactgg tcggaggatt cacctttccc gcatttcgca gagcgggacg gcgcttgatt
     4441 agacgatgac aaaaatgggc ctgcagcggc cggaagtcca ataaatcccg ccaaatggcc
     4501 ggaagcaggg acgaggggca ggggaggaag ggtgaggggt accggtaggt ggtccccggt
     4561 tggcagtagg cggtaggcgg tccccggttg gcagtaggcg gtagg
//
LOCUS       DAOTON010000108         4598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15378_length_4598_cov_2.675545, whole genome shotgun sequence.
ACCESSION   DAOTON010000108 DAOTON010000000
VERSION     DAOTON010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15378_length_4598_cov_2.675545"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..859)
                     /locus_tag="P5292_03205"
     CDS             complement(74..859)
                     /locus_tag="P5292_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38164.1"
                     /translation="MTTEYRFRFSQFRLFLLVLACSVVGRSASAQKSVRDSSISMTMI
                     GAIVGVQSPGGDLSDRFGTNFNIGGTFQYKTRTNFLFGVDGYFLFGPDVKDEGLLRYF
                     LNSSGAITGLDGQFANILLYERGMKFDLKAGRIFPVIGPNPNSGITLTLGAGWIQHKI
                     RIDNKSEAIPALSGDYLKGYDRLTGGFALTGFAGYTNFGNKRLVNFFLGVEVTRGFTH
                     NLRRFNFDTREQDDRLRNDLFFGIRGGWLFPLYRRSPRAYYYN"
     gene            complement(856..1392)
                     /locus_tag="P5292_03210"
     CDS             complement(856..1392)
                     /locus_tag="P5292_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HRS38165.1"
                     /translation="MSDPGEIYLLECLKSFKGIKSTSEKAIIQIKDEELHWSSDPESN
                     SVAIILRHISGNLRSRFTDFLTTDGEKPDRNRDAEFVDSGISREVLLSEWETSWKILF
                     DTLGQLTSASLTQTVYIRNEPHTVIRALQRQLVHYAYHSGQIVYLCKQIRAGNFKSLT
                     IPRGASGDFLHSPPTSAT"
     gene            complement(1389..1889)
                     /gene="cdd"
                     /locus_tag="P5292_03215"
     CDS             complement(1389..1889)
                     /gene="cdd"
                     /locus_tag="P5292_03215"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRS38166.1"
                     /translation="MKKEIQLQSTFTEYDDISDLPEQDRLLVAEARKSVGQAYAPYSH
                     FQVGAALRLENGVILRGNNQENASYPIGLCAERVAVFAAGANYPGIRILSLAITANSN
                     HFHVNKPITPCGACRQAIAEYEHRYQQPIRLIMVGESGKVLVADSIQHFLPYQFNADD
                     LQVKTS"
     gene            2110..2550
                     /locus_tag="P5292_03220"
     CDS             2110..2550
                     /locus_tag="P5292_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38167.1"
                     /translation="MLHNIKSVLAALLIVSGLFLSSCDKDAGEGGTSTIKGKVMVYDY
                     DASFQACQDTYPAFKEDVYIIYGDDRSTYDDKMETSYDGSFEFKYLRKGKYKIFAYTK
                     DTTGASNGTYNDSRPRLPSMVEVEITSNRSEVVAKDIIMLDNNQ"
     gene            2565..3122
                     /locus_tag="P5292_03225"
     CDS             2565..3122
                     /locus_tag="P5292_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38168.1"
                     /translation="MLLVSFLVIGTTVQVSAQKSKDAKKYKLKAAKITSTITRDGKEV
                     TVVELDERYDSEGNIIEEVDFDSEGAFKSKITRTFNKAGDPTEESYYTSDGKLKKRHV
                     MKYNAAGDKVEEQRFDANNVLQEKSVYTYNAKGERTAEIVLDGKNNTVEKSLMIYDAK
                     GMRIERKTFDAKGTLVETKKYQYEY"
     gene            3127..3882
                     /locus_tag="P5292_03230"
     CDS             3127..3882
                     /locus_tag="P5292_03230"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRS38169.1"
                     /translation="MIALDTLLAPFDPISLKQMDNVKLLDRVDTKFMFREELLPAILE
                     RMRPEYYVLEINGQRYNHYETLYFDTHNFGLYLSHHNGKLNRFKFRARRYVESNLHFF
                     EVKFKNNKGRTIKDRIKRPEISPVIADKAEELVRSASNVDPSTLRAQLWVDYVRITFV
                     SKTSQERLTVDTRLTYRNDGHQVDYNGLVIAEVKQGSARDKSVFVDLMREHSIPQRSI
                     SKYCLGVISLNPDVKRNRFKPTLLYLQKLLRAS"
     gene            3879..4496
                     /locus_tag="P5292_03235"
     CDS             3879..4496
                     /locus_tag="P5292_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRS38170.1"
                     /translation="MNDAMTGFELFDKLSDKFFIRLLIDIVSMLVLVRLIYFRIYKKK
                     DYLFTFFLFNIIIFIITYLLNKVDMSMGAAFGLFAVFSMLRYRTEGITTKDMTYLFIV
                     IAIGLICAVSKATYFELGVINLILIGFTYALDGNWLVRNEMIKTVQYENIDLIRPENY
                     QLLLEDLRKRTGLNIHRASINKIDFLKDIAVVKVYYYEDTVRHSK"
BASE COUNT         1131 a         1166 c         1253 g         1048 t
ORIGIN      
        1 ttatttccga gcaaggctgc gagccggata ccggctccgt agaggagaat tcccaggttg
       61 taaagagcgt acatcaatta taatagtaag ctcggggcga ccgccgataa agcggaaaca
      121 accaaccacc acggatgccg aaaaataagt cgttgcgcaa tcgatcatcc tgctcccggg
      181 tatcgaaatt gaatcgacgc agattatggg tgaatccccg ggttacttcc acgcccagga
      241 agaagttgac gagtcgcttg tttccgaaat tggtgtaacc cgcaaagccg gtgagcgcga
      301 atccccccgt cagccggtca taccctttca ggtagtctcc gctcaacgcc ggaatggctt
      361 ccgatttgtt gtcgatgcga atcttgtgtt ggatccaacc ggctcccaat gtcagggtga
      421 tgccggagtt ggggttcgga ccgattacgg gaaatatcct gccggccttc aggtcgaatt
      481 tcatgccccg ctcgtagagg agtatgttcg cgaactgacc gtccagaccg gtgatagcgc
      541 cggaactgtt caggaaataa cgtaagaggc cttcatcctt gacgtcgggg ccgaacagaa
      601 aatacccgtc aactccgaac aggaagttcg tgcgtgtctt atattggaag gtgccgccga
      661 tgttgaaatt cgtcccgaac cggtccgata gatcaccacc gggcgactga acgccgacga
      721 tcgcgccgat catggtcatg gagatgctgg aatcccgcac acttttctgc gcggaagcac
      781 ttcggccgac gactgaacac gccagcacta gcaggaagag ccggaattgg ctgaagcgga
      841 atcgatattc ggttgtcacg tcgcggaggt gggaggtgag tgcaggaaat cgccggaagc
      901 gccgcgcgga atagtgagcg atttgaaatt gccggctcgg atttgtttgc aaaggtacac
      961 gatctgaccg ctgtggtaag cgtagtgtac cagttgccgc tgaagtgcgc gtatgaccgt
     1021 gtgcggttcg ttgcggatat atacggtctg tgtgagcgag gcggaggtta gctgtcccaa
     1081 cgtatcgaac aggatcttcc aggaggtctc ccattccgat aagaggactt cccgggagat
     1141 gccgctgtcc acgaactccg cgtcgcggtt gcggtcgggt ttttctccat cggtcgtcag
     1201 gaagtccgta aagcggctcc ggagattgcc ggatatatga cgaagaataa tggccaccga
     1261 gttggattcc ggatcagacg accaatgcaa ttcctcatcc ttgatctgga taatggcttt
     1321 ctcggacgtg ctcttgatgc ctttgaaact cttgaggcat tccagcagat agatttcacc
     1381 cggatcactc atgaggtctt gacttgcagg tcatccgcgt tgaattggta cggcaggaaa
     1441 tgctggatac tgtcggcgac caggactttt ccggactcgc ccaccatgat gagccggatc
     1501 ggttgctgat accggtgctc gtattcggcg atcgcttgtc ggcacgcgcc acaaggggtg
     1561 atgggtttgt ttacgtggaa gtgattggag ttagcggtaa tggcgaggga gaggatgcgg
     1621 atgccgggat aattcgcgcc tgccgcaaag acggctacac gttccgcgca gagcccgatc
     1681 ggataggacg cgttttcctg gttgttgccc cgcaggatga caccgttttc caggcgcagc
     1741 gcggcgccta cctgaaaatg actgtaaggt gcatacgcct gaccaaccga tttccgggct
     1801 tccgcgacta acaaccggtc ctgctcgggt agatcggaaa tgtcatcgta ctccgtaaag
     1861 gtcgattgaa gttggatctc ttttttcatg gaagagcgaa tgtaggaaga cgacgggaga
     1921 ctgggtagag aacgcgtagg caattgtcgg aaggaagtaa gtttcggcaa ggattttgcc
     1981 cttcctttaa caacatttcg gggcgccttt ccgtatatct ctgaccgaac gccatatcct
     2041 ggtccacaat ggcggataaa ttcagcatat ttgcagttgc ctattaatag aaatgatctg
     2101 ataatgagaa tgttacataa tatcaagtca gttttagccg cgctgctgat cgtctccggc
     2161 ctgtttctct cttcctgcga taaagatgcg ggtgaggggg ggacttcgac tatcaagggc
     2221 aaggtgatgg tgtatgatta tgacgcttct ttccaggctt gtcaggacac gtatccggcc
     2281 ttcaaggagg acgtctacat catctacgga gatgaccggt cgacctatga cgataaaatg
     2341 gagaccagtt acgacggcag ctttgaattc aagtacctgc gcaaagggaa gtacaagatt
     2401 ttcgcctata ccaaggacac gaccggtgct tccaacggca cctataacga ttcccgcccc
     2461 aggcttcctt cgatggtcga agtggagatc acttccaacc ggtccgaagt cgttgctaag
     2521 gatatcatca tgctcgacaa taatcagtaa tgcgaatgta caggatgtta ttagtgtcgt
     2581 tcctcgtgat cggcacaacg gttcaggtat ccgcgcagaa aagcaaggat gccaagaaat
     2641 acaagctcaa agccgcgaag attaccagta cgatcacgcg tgatgggaaa gaagttaccg
     2701 tggtagaact ggatgagcgg tacgattcgg agggcaacat catcgaggag gtggacttcg
     2761 attccgaagg cgcgttcaaa tcgaaaatca cacgcacctt caacaaggcc ggagacccga
     2821 ccgaagaatc ctactatacg agcgacggga aattgaaaaa gcggcatgtc atgaaataca
     2881 atgccgccgg cgataaggtg gaggagcaac gcttcgacgc caacaatgtg ttgcaggaaa
     2941 agtcggtcta tacgtacaat gcaaagggcg aacggacggc ggagatcgta ctggatggca
     3001 agaacaatac ggtggagaag tcgctcatga tctacgatgc caagggcatg cggattgagc
     3061 gtaagacctt tgacgccaag ggtaccctgg tggaaacgaa aaaatatcaa tacgaatact
     3121 gattccgtga tcgcgctcga tacactgctg gctccgttcg atccgatctc cctcaagcag
     3181 atggacaatg tcaaactgct cgaccgggtc gatacgaagt tcatgtttcg cgaggaactc
     3241 ctcccggcca ttcttgagcg aatgcgcccg gaatattatg tcctggagat caacgggcag
     3301 cgctacaatc actacgagac gctctacttc gatacgcata atttcggcct ctacctgagc
     3361 caccacaacg gcaagctcaa ccgcttcaag ttcagagcca ggcggtatgt ggaaagcaac
     3421 ctgcacttct tcgaagtgaa attcaagaac aacaagggcc gaacgatcaa ggaccgcatc
     3481 aaacgccccg agatctctcc cgtgatcgcg gataaagccg aggagttggt acgcagtgct
     3541 tccaatgttg atccctccac actccgcgcg cagctctggg tggattatgt ccgcatcacg
     3601 ttcgtatcca agacttcgca ggagcgtctc acggtcgata ccaggctgac gtaccgcaac
     3661 gacggtcatc aggtcgacta caacggtctc gtgattgccg aagtgaagca ggggagcgcc
     3721 cgcgacaaga gcgtattcgt cgatctgatg cgggagcatt ccattcccca gcgatccatc
     3781 agtaagtact gcctcggggt catcagcctg aatcccgacg taaagagaaa cagattcaaa
     3841 cccacgcttt tatacctcca aaaattactt cgtgcatcat gaatgacgca atgaccggct
     3901 tcgaattatt cgacaagctt tccgacaagt tcttcatccg cctcctcatc gacatcgtct
     3961 cgatgctggt actggtgcgt ttgatctatt tccggatcta caagaagaaa gattatctct
     4021 tcaccttttt cctgttcaac atcatcatct tcatcatcac ctacctgctc aacaaggtgg
     4081 atatgtcgat gggagctgcg ttcggacttt ttgccgtctt cagcatgttg cggtaccgta
     4141 ccgagggcat tacgacgaag gatatgacct acctcttcat cgtgatcgcg atcggcttga
     4201 tctgtgcggt aagtaaggcg acctatttcg aactgggtgt gatcaacctc atcctgatcg
     4261 ggttcaccta tgcgctggat ggaaactggt tggtgcgcaa cgagatgatc aagacggtac
     4321 aatacgagaa catcgatctg atccgtcccg agaattacca gttgctcctg gaggacctcc
     4381 ggaagcgtac cggcctgaat atccaccgtg ccagcatcaa caagatcgac tttctgaaag
     4441 acatcgcggt ggtaaaggtg tattactacg aagacaccgt gcgccattcc aaatgatgcg
     4501 gagtatccgg ctgttcgtgt tagccctgtt gatcggctca tcggctgctg cctttgagcc
     4561 ggacagcgat tttggcgcct ggttcagtgt caatgtcc
//
LOCUS       DAOTON010000109         4521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15685_length_4521_cov_2.565383, whole genome shotgun sequence.
ACCESSION   DAOTON010000109 DAOTON010000000
VERSION     DAOTON010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15685_length_4521_cov_2.565383"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4424)
                     /locus_tag="P5292_03240"
     CDS             complement(<1..4424)
                     /locus_tag="P5292_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38171.1"
                     /translation="MPWWDDMGTGTNGNVQYQLLGTSPNRKLVVEWRVRNIGETGNYT
                     KTYQLWLFESNFAIQFVYGTGTAGSSASIGMSTSATEYQSVTTTSNTASTTTPANTQS
                     TFPASGRYYLFTPASCAGTPSPGNTLASANPVCSGSALTLSMSTPPSSIGITYRWQTS
                     PDGSTWTNVAGAAGNNVTLSANPTVATYYRCRVTCSASGLNGFSTPLLVNVVNCYNMQ
                     NGSLSNVCSARFYDSGGLAAAYANAESYTLTIYPTPGNLLRVQFNSFVTESGYDYLSI
                     YNGNSTAAPLLLNTSGSTVPGTYTSSAVDGSLTFRFTSDLSITYAGWDALLSCVSLPV
                     CSGTPTAGTISGTSGLCAGGTTTLTASGFSSGVSGLSFQWEESDDNGVADPWANVVGG
                     SGANTNSYTSPALSVSRYYRMRVTCSNGGGTATTSGYLVTANPLPTVAIAANPGTTIC
                     GPGAINLSASGADTYNWSPASGLNATTGASVTATLSSSTSYTVVGTITATGCTATQSV
                     SLNLLPGYSVNATANPVNICSGGSAQLNAVAQMPSSSYLLNPISPALVSTAGFATGPA
                     SDDAIVGPITLPFNFNFFGSSEDSLYISSNGHIIFGNSAPANQLAGLNIPDATDPDNV
                     IALCWADLNPGTSGSVTYGTVGTAPNRIFVISWNAVPFYLQSGSNVTGQIQLYEGSNI
                     VEVHISVINRGASTSNTVLGIENATGSSGLAPASRNGGTWSVSSPEAWRFSPYVPGYT
                     YAWTPSTGLDNPTIPMPIASGVSGTTVYTVTVTADGVCTATATALVNEGVALSATASA
                     LPASVCEGQSTQLSGAASGGGEPFAYSWSDGVTEIGTTQLLTITPPVGSSTYYLTVAD
                     ACGGSTSTTVVVTALPGPVLALQPVSAVYCGTPLALTASSGGGTTFGWSPATGLNATT
                     GSSVSASPATATTYTVTATATNGCTISGTIPITIGTPLTVSVGADTTQGCVPFGSVLR
                     ACVNPTFNTYTLDSAATDYSPVGSTAFITLSSADDGDAAVNLPFPFNFYGVNYTSIRV
                     GSNGYIGMGTTPVTSLANQALPSATAPNGIIALCWDDLLHSGLATGIDTFTVGTAPNR
                     KFVVRYNAGAVAFYNIGSQTGSLGGKIVLHEGTGVIEVRIDVMNKGVRIDNQTLGIED
                     AAGLAAVTPPGKNNLDWLQSTPRTYRFTPAAQQCNNTGISFDWTPAATLHATNLDTAI
                     ITGLNSTTIFTVTASNASGCSAQDTVQIFVSPQAPTPVISSSGPLSFCPGGSVTLSSS
                     STAFPNYWSTGETTNSITVNTTQTVSVYSSDAFCPSGRAYVDVVRFDTVPPLINVSGG
                     GLALCSGNRDLLSDGAPLFVAWSWSTAETSQQITIMNPGTYAVAATDTNGCVTHAEIT
                     FTAGVAPVAPVISTLSSLSVCNNEYVTIDSDIPDGLTWFPTFDNTPSIAYNLSPPGVY
                     DFYVSRDSLGCTSESNHLVFTVNPTPEVYFFLPADSACV"
BASE COUNT         1107 a         1170 c         1299 g          945 t
ORIGIN      
        1 acacaggcgg agtccgccgg caggaagaaa tatacctccg gagtcggatt gaccgtaaag
       61 accaggtgat tggactccga ggtacaaccc agggagtcgc gcgaaacgta gaagtcgtat
      121 actcccggag gtgaaaggtt gtaggcgatc gacggtgtat tgtcaaacgt cgggaaccag
      181 gtgagcccat ccggaatatc ggaatcgatc gtcacgtatt cattgttgca tacactgagc
      241 gaactcaagg tggagatcac aggggccacc ggagcgacac ctgccgtgaa cgttatctcc
      301 gcatgcgtta cacagccatt ggtatccgta gcggccactg cataggtccc gggattcatg
      361 atcgtgattt gctgagaggt ttcggcggtg ctccaactcc aggctacgaa gagcggtgcg
      421 ccatcggaga gcagatcacg gttgcccgaa catagcgcga gtccgccgcc ggagacgttg
      481 atcagcggcg gtaccgtatc gaatcgcacg acatcgacat aagcccggcc ggaaggacaa
      541 aaagcatccg aactgtaaac cgaaacggtc tgggtagtat ttactgttat cgaattcgtt
      601 gtttcgccgg tactccagta attcgggaaa gccgtactgg aagaacttaa cgtcacgctt
      661 ccgcccgggc agaaggaaag cggtccgctg ctcgaaatca ccggtgtagg agcttgtggc
      721 gatacgaata tctgtaccgt atcctgagca ctgcatcccg aagcgttcga cgcggttacg
      781 gtaaaaatgg ttgtgctgtt cagtcctgtg ataatagcgg tgtccaggtt tgttgcatgc
      841 agggtggcag ccggtgtcca gtcaaacgag ataccggtgt tgttgcactg ttgtgcagca
      901 ggagtgaacc gataggttcg tggtgtcgat tgaagccaat ccagattgtt ctttcccgga
      961 ggcgttacag ctgccaatcc ggccgcatct tcgattccaa gcgtctggtt atcgatccgt
     1021 acccctttgt tcatcacatc gatcctgact tcgatgactc cggttccttc atgtaaaacg
     1081 atcttaccgc ccaaggaacc ggtttgtgat ccgatgttgt agaacgcgac cgctcctgcg
     1141 ttataccgga cgacaaactt tctattgggc gcggttccca ccgtaaatgt atcgatgccc
     1201 gtggccagtc cgctgtgaag cagatcgtcc caacacaagg cgatgattcc attaggcgcc
     1261 gtggctgaag gaagcgcctg attggccagg gaggtcaccg gggtggttcc catcccgatg
     1321 tagccattcg atccaacgcg tatcgaagtg taattcacgc catagaagtt gaacgggaac
     1381 ggcagattga ccgccgcatc gccatcatct gcactggaca gtgtaatgaa agcggttgat
     1441 ccgactggtg aataatcggt ggcagcagaa tccaacgtat aggtattgaa ggtcggattg
     1501 acacaagccc gcagtacaga gccgaaggga acacatcctt gtgtagtgtc cgcgccgaca
     1561 ctaaccgtca gcggggttcc gatcgtaatg ggaatagtac cactaatcgt acaaccatta
     1621 gtagcagtcg cggtgacagt atacgtcgtg gcggtcgccg gactcgcact aacagaagag
     1681 ccggtggtag cgttcaatcc cgttgcaggc gaccagccaa aggttgttcc gccgcccgaa
     1741 gatgccgtaa gcgcaagtgg tgttccgcaa tacaccgcgc taaccggttg cagggcaagt
     1801 accggccctg gaagggcggt gacgacaaca gtagtggacg tgctgccccc acaggcgtcg
     1861 gcaacggtca ggtaataggt ggaagatcca accgggggag tgatcgtcag caattgcgtg
     1921 gtcccgattt cggttacgcc gtcggaccaa ctataggcga atggttcccc gccaccggaa
     1981 gcggccccgc tcagttgcgt cgattgccct tcgcataccg aagcaggaag cgcggaagcc
     2041 gtagcactca gtgcgactcc ttcgttcacc agggcagttg ccgttgccgt gcacacacca
     2101 tccgccgtaa ccgtcacggt ataaacggtc gtgcctgaca ctccactggc gatcggcatc
     2161 ggaatggttg gattatcgag tccggtcgaa ggggtccatg cataggtata acccggaacg
     2221 taaggtgaaa atcgccaggc ctccggactg ctcaccgacc atgttccgcc gttacgactg
     2281 gcgggcgcaa gtccacttga accggtagca ttttcaatcc ccaaaacggt atttgaggtc
     2341 gatgcacccc ggttgattac gctgatgtga acctccacaa tattggaacc ttcgtacaat
     2401 tggatctgac cggtgacgtt gcttccggac tgcaggtaaa acggtacggc gttccaactg
     2461 atcacaaaga tcctgttggg tgctgtgccc accgtaccat aggtgaccga tccggaagtg
     2521 cccggattga ggtcggccca gcagagcgcg atgacgttat ctggatctgt tgcatccggt
     2581 atgttgaggc ccgctaactg attcgcgggt gccgaattac caaatatgat atgaccgttc
     2641 gaggagatat acagcgaatc ctccgatgac ccgaagaaat tgaagttgaa gggcagtgta
     2701 atggggccaa cgatcgcatc atcggacgcg gggccggtcg cgaatcctgc ggtggaaacc
     2761 aatgcaggag aaatcggatt caacagatag gaacttgagg gcatttgagc aacagcgttg
     2821 agttgagcgc ttccaccgga acaaatgttg accggattag ccgtcgcatt caccgaatag
     2881 cccggcaaca aattgagtga aacactttga gtcgcagtgc accccgtagc agtgatcgta
     2941 ccgacaactg tataactggt agaactcgaa agggtggccg tgactgaagc accggtagta
     3001 gcgttcaagc cggaagcagg tgaccagttg taggtgtcag ctccggaagc gctcaggtta
     3061 atcgcacccg gtccgcaaat ggtcgtaccc ggattggcag caattgccac tgtaggaagc
     3121 ggattagcag taaccaaata accggaagtc gtggcagtac cgcctccatt tgaacaagtg
     3181 acccgcatcc ggtaatacct ggaaacactc agggccgggc tggtatagct attcgtattg
     3241 gcacccgatc caccgacgac attcgcccag ggatcagcca cgccattgtc atccgactcc
     3301 tcccactgaa acgaaagccc tgaaacaccg gacgaaaatc cggaggcggt aagggttgtc
     3361 gttcctcctg cacacaggcc ggatgttcca ctgatagtac cggcagtcgg tgttccacta
     3421 catacaggaa gcgaaacaca actcaataag gcatcccaac cggcataggt gatactgagg
     3481 tcggacgtga accggaatgt caatgatcca tccaccgccg aagaggtata ggttccgggc
     3541 acggtggagc cggaagtgtt gagcaaaagc ggcgcggccg ttgaattgcc attgtaaatg
     3601 gacaagtaat catacccgct ttcggtgacg aaggagttga attgcacgcg aagcaaattt
     3661 ccgggtgtag gataaatggt aagagtataa gattccgcat tagcataagc agcggccagt
     3721 cctcccgaat cataaaacct ggccgaacag acgttggaca aagagccatt ctgcatgtta
     3781 tagcaattca ccacattcac caacaaaggc gttgagaagc cgttgaggcc ggaagcagaa
     3841 caggtgacac ggcaacggta gtacgttgca accgtcgggt tcgcggataa ggtaacgttg
     3901 tttccagcgg caccggctac gttggtccag gtcgaaccat ccggcgaagt ctgccatcgg
     3961 taggtgatgc cgatgctgga aggaggagtt gacatcgata gcgtcaaagc gctgccgctg
     4021 cagaccgggt tggcagaagc aagggtgtta cccgggctgg gtgttccggc acaggaggca
     4081 ggcgtaaata aataataccg accggatgcg ggaaatgtgg actgggtatt ggcaggagtt
     4141 gtcgtcgaag cggtattcga tgtggtcgta acactctggt actcggtagc cgatgttgac
     4201 atgccaatcg atgccgagga tccggcggtg cctgtcccat acacgaattg aatggcaaag
     4261 ttgctttcga acaaccagag ctgataggtc ttggtatagt tgcccgtctc tcctatgttc
     4321 cgaactcgcc attcaacaac caatttccga ttaggcgatg ttcccaataa ttggtactga
     4381 acgttcccgt ttgtaccggt gcccatgtca tcccaccagg gcatcagggc cgggaaactt
     4441 ggcggtgctg taattgaatt tgagtacgac gtactcggcg atgttccgaa accgaacagg
     4501 ccgtttgcgt tcacccaaaa a
//
LOCUS       DAOTON010000110         4499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15774_length_4499_cov_3.443744, whole genome shotgun sequence.
ACCESSION   DAOTON010000110 DAOTON010000000
VERSION     DAOTON010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15774_length_4499_cov_3.443744"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="P5292_03245"
     CDS             complement(<1..235)
                     /locus_tag="P5292_03245"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRS38172.1"
                     /translation="MKNFQRQVLVTGGAGFIGSHVVRLLVNNYPQYRIINLDKLTYAG
                     NLANLRDVEDLPNYSFVRGDIVDGDFIQSLFDRE"
     gene            complement(232..1263)
                     /gene="galE"
                     /locus_tag="P5292_03250"
     CDS             complement(232..1263)
                     /gene="galE"
                     /locus_tag="P5292_03250"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRS38173.1"
                     /translation="MNILVTGGTGYIGSHTAVELIQRGYNAILLDNLSNSREEVVHAI
                     GEITGKKIDFFNIDLCDKKELDKFFSVYKIDAVIHFAAFKAVGESVEDPLRYYRNNLE
                     SLINLLEIYRDRGLDNFLFSSSCSVYGQASKLPVSEDAPLLKAESPYGNTKQIGEEIL
                     ADTIRVSKFRGIALLYFNPAGAHPSALIGEYPLQAPNNLVPVITQTAIGKRPGMTVFG
                     SDYDTPDGTCVRDYIHVVDIARAHIAAMERLIQRKNKTPFEIFNLGTGTGFTVLQTIQ
                     AFEKVTGKPLNYTLGPRRPGDVEKVWADTTLVSKELGWKAELGIEEIMRSAWAWELRL
                     QEESKKQLA"
     gene            complement(1265..2554)
                     /locus_tag="P5292_03255"
     CDS             complement(1265..2554)
                     /locus_tag="P5292_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRS38174.1"
                     /translation="MVQELLKKEKKLAILGLGYVGLPIALEFAKKIKVIGFDINEARL
                     DKMRNSIDPSGELDTEAFNGCDIEFTSSIDKLREASFFIVTVPTPIDEHNLPDLKPLL
                     GATRFIGKVLKKGDFVVYESTVYPGCTEEDCLPILEDESGLKVNADFKLGYSPERINP
                     GDKEHTITRILKVVSGSDKEALDTIASVYEIIVKAGVFRASSIKVAEAAKIIENTQRD
                     VNIALMNELSIIFNKMGINTYDVLQAAGTKWNFIRMQPGLVGGHCIGVDPYYLTYKAQ
                     ELGYHAQVINSGRYVNDSMGFYVAKQTVKKIIAAGKDVSKARALVMGATFKENVDDIR
                     NSKVADVVKELQSYGVQTDVVDPRADSNELQHEYGFGLLEKPGTGYDAVIVAVNHSEY
                     QQLDEAYFRSLLQPDGLVVDIKGMYKGKIQSINYWSL"
     gene            complement(2589..3626)
                     /locus_tag="P5292_03260"
     CDS             complement(2589..3626)
                     /locus_tag="P5292_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS38175.1"
                     /translation="MIDSNKRVRFGVVGAGHIGKRHAEMIRRNGESELVAMCDVRPQS
                     EVQLDPSIPLYPSLEAMLEAHPDLDVINVCTPNGLHGEHTLKALEARKHVVIEKPMAL
                     TVAECEKIIFKALQVSRQVFCVMQNRYSPPSVWIKELVEAGTLGRIFMVQLNCYWNRD
                     NRYYTGKNWKGTPDLDGGTLFTQFSHFIDIMYWLFGDITEIQARFDDFTHENLTAFED
                     SGLVNFRFVNGGMGSINYSTAVWDKNLESSITIIGEKGSVKIGGQYMNEVERCHIDGY
                     TMPELPPASPANDYGAYKGSAANHHFVIENVIDTLKGRTSATTNALEGLKVVDIIERI
                     YALRKLPKLNR"
     gene            complement(3629..4207)
                     /locus_tag="P5292_03265"
     CDS             complement(3629..4207)
                     /locus_tag="P5292_03265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRS38176.1"
                     /translation="MDKNYFAHATAVVDDKAVIGTGTKIWHFSHIMDGATIGENCNIG
                     QNVVISPDVTLGKNVKVQNNVSIYTGVTCADDVFLGPSMVFTNVNNPRSAITRRNQFL
                     KTHVGTGATIGANATVVCGHNIGEFAFIGAGAVVTKDVPAYALVVGNPARQLGWMSEY
                     GHRLKFDANGIAVCPESKQEYKLEGNKVRRIK"
     gene            complement(4282..>4499)
                     /locus_tag="P5292_03270"
     CDS             complement(4282..>4499)
                     /locus_tag="P5292_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015024466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD-dependent epimerase/dehydratase"
                     /protein_id="HRS38177.1"
                     /translation="EIIRLTGTNQKVVYHPLPQDDPKQRQPDITRAREVLGWEPRIDR
                     SEGLRRTYEYFRGLSPEELRNSAHRFA"
BASE COUNT          940 a         1129 c         1247 g         1183 t
ORIGIN      
        1 gttcgcgatc gaatagggat tggatgaaat ccccgtcgac gatatcgccc cggacaaacg
       61 aatagtttgg aagatcttct acatcgcgga gattggccag gttgcccgca taggtcagct
      121 tgtccagatt gatgatccgg tactgtgggt agttgtttac cagcagtctg acaacgtggg
      181 agccgataaa tcctgctccg cccgtgacga gcacctgtcg ttggaaattc ttcatgccaa
      241 ttgttttttt gactcttcct gcaaacgaag ctcccacgcc caggcggaac gcatgatctc
      301 ttcgatgccg agctccgctt tccagccaag ttctttgcta acaagcgtcg tgtcggccca
      361 gactttctcc acatcgccag ggcgacgcgg ccccagggtg tagttgagtg gtttcccggt
      421 cactttttcg aaggcctgga tggtttgcag aacggtaaat ccggttcctg tgccaaggtt
      481 gaagatctca aagggtgttt tattcttgcg ctggatgagg cgctccatag cggcgatatg
      541 cgcacgggcg atatccacca catgtatata atcccggacg caggtgccat ccggagtatc
      601 gtagtcgctt ccgaaaacgg tcatgccggg tcgtttaccg attgcggtct gagtaatgac
      661 gggcaccaga ttgttcggtg cctgaagcgg atattcacca atcaatgcgc tggggtgggc
      721 acccgcggga ttgaaataga ggagggcgat gccacggaat ttgctgaccc gtatcgtgtc
      781 ggccaagatc tcttctccga tttgtttagt gttgccgtaa ggtgattccg ctttcagcag
      841 gggggcatct tcagaaacag ggagcttgga tgcctgaccg tacacggaac aggaagacga
      901 gaagaggaaa ttatcaagtc cgcgatcgcg gtagatctcc agcagattga tcagcgattc
      961 caggttgttg cggtagtacc gcagcggatc ttcgacggat tcgcctaccg ctttgaaggc
     1021 ggcaaagtgg atcaccgcat cgatcttata aaccgaaaag aacttgtcga gttccttttt
     1081 gtcgcagagg tcgatgttaa agaaatcgat tttttttcct gtaatttctc caatggcgtg
     1141 caccacctct tcgcgggaat tgctgaggtt gtcgagtagg atggcattgt accctcgttg
     1201 gatcaattcg accgcggtgt gcgagccgat gtaaccggtt ccgcctgtaa ccaggatgtt
     1261 catgtcagag actccagtag ttgatggatt ggatctttcc tttgtacatg cctttgatat
     1321 cgacgaccag gccgtccggt tgcaagaggc tgcggaagta ggcttcgtcg agttgttggt
     1381 attcggaatg gttaacggct acgatcacgg cgtcgtatcc agtgccaggt ttctccagta
     1441 atccaaatcc gtattcgtgt tgaagttcat tcgaatcggc acgcgggtcg actacatcgg
     1501 tctgcacccc gtaggattgg agttccttga ccacatcggc gactttactg ttacggatat
     1561 cgtctacgtt ttccttgaag gtggcgccca tgaccagggc gcgggccttg gagacatctt
     1621 tccccgcggc gatgatcttc ttgaccgttt gctttgccac atagaaaccc atgctgtcgt
     1681 tcacgtagcg gccgctgttg atgacctggg cgtggtagcc gagttcctgc gctttgtagg
     1741 tcaggtagta gggatcgacg ccgatgcaat ggccgccgac gagtccgggt tgcatgcgga
     1801 tgaaattcca cttggtcccg gcggcttgaa gtacgtcgta ggtattgatg cccatcttgt
     1861 tgaagatgat ggacaattcg ttcatcagcg cgatgttcac gtcgcgttgc gtgttctcga
     1921 tgatcttggc ggcttctgcg accttgatgg aggatgcccg aaatactccg gcctttacga
     1981 tgatctcata aacggaggcg atcgtatcga gcgcttcctt gtcggatccc gaaaccactt
     2041 tgaggattct ggtgatcgta tgttccttgt cgcccggatt gatacgttcc ggagagtatc
     2101 ccaatttgaa atcagcgttg accttcaggc ctgattcatc ctcaagaatc ggcaggcaat
     2161 cctcttcggt gcatccggga tagacggtgg attcatacac cacgaaatca cctttcttga
     2221 gcactttgcc gataaagcgt gtggctccca gaagtggttt caggtcgggc aggttgtgct
     2281 catcaatcgg cgttggtacc gttacgatga aaaaacttgc ctcgcggagt ttatcgatgg
     2341 atgaggtgaa ttctatatca catccgttga aagcttccgt gtccagttcg ccgctcgggt
     2401 caatgctatt gcgcatctta tccaaccggg cttcgttgat gtcgaagccg atgaccttga
     2461 tctttttggc gaactccagc gcgatcggga gcccgacata gccgagtccc agaatggcaa
     2521 gttttttttc tttcttgagt aattcctgaa ccatatggat gcttcttccg gaaaattccg
     2581 aaaggtattt agcgattgag ttttggtagt ttgcgcaaag cgtaaatgcg ctcgatgatg
     2641 tcgaccactt tcaggccttc caacgcgttg gtagtggcgc tcgtgcggcc tttcaaggtg
     2701 tcgataacgt tttcgatgac gaagtggtga ttggcggcag aacccttata ggctccgtaa
     2761 tcattggcgg ggcttgccgg tggtaattcc ggcatggtgt aaccgtcgat atgacaacgc
     2821 tccacttcat tcatgtattg tccaccgatc ttgacgctgc ctttttcgcc gatgatggta
     2881 atgctcgatt caaggttttt gtcccaaacc gcggtcgagt agttgatgct gcccatgccg
     2941 ccgttgacga aacggaagtt caccagaccg gaatcttcaa aggcggtgag gttctcgtgc
     3001 gtaaaatcgt cgaagcgtgc ctggatctcc gtgatatcgc cgaacagcca gtacatgatg
     3061 tcgatgaaat gtgaaaactg ggtaaagagc gtcccgccgt caaggtcggg tgtgcctttc
     3121 cagttctttc cggtgtagta gcggttgtcg cggttccagt aacaattcaa ttgtaccatg
     3181 aagatgcgac cgagcgtgcc cgcctcaacc agttccttga tccagacaga gggaggggaa
     3241 tagcgattct gcatgacgca gaacacttgc cgggaaacct gcagtgcttt gaagatgatc
     3301 ttctcacatt cagctaccgt gagcgccatc ggtttctcaa taacgacatg cttgcgcgct
     3361 tcgagcgctt tcagcgtgtg ttcgccatga agaccgttcg gggtacatac attgatgaca
     3421 tccagatccg gatgggcttc gagcatggcc tcgagcgagg ggtagagcgg gatggaagga
     3481 tccagctgaa cctccgattg tggtcgaacg tcgcacatgg cgacaagttc ggattcgccg
     3541 ttcctgcgga tcatttccgc atgccgtttt ccgatgtggc cggcaccgac tactccgaag
     3601 cggacgcgtt tgtttgaatc gatgatgctc acttgatgcg acgaacctta ttgccttcca
     3661 atttatattc ctgtttgctt tcgggacaaa cggcaatgcc gttggcatcg aacttcaggc
     3721 gatgaccgta ttcactcatc cagcccagtt gccgggcggg gttgccgact accagcgcgt
     3781 aggcaggaac atccttcgtt accacggctc ccgcaccgat gaacgcgaac tcaccgatgt
     3841 tgtgtccgca cacgaccgtc gcattggccc caatggtcgc gcctgtaccc acatgcgttt
     3901 tgaggaattg gtttcggcgc gtgatggcgc tgcgcggatt gttgacgttt gtgaagacca
     3961 tggatgggcc aaggaataca tcgtcggcac aggtcacgcc agtatagatc gatacgttgt
     4021 tctgaacctt gacattcttt ccgagcgtga cgtcgggtga gatcaccacg ttctgtccga
     4081 tgttgcagtt ctcgcctatt gtggctccat ccatgatgtg cgaaaagtgc cagatcttcg
     4141 ttccggtgcc aattaccgct ttgtcgtcga ccaccgcagt cgcgtgagcg aagtagttct
     4201 tatccatagt ctgcttattc ccgctaagtt acgaaatcgg gaaggaatgt attattctga
     4261 taatcaatta tgtaaatatg atcaagcgaa cctgtgggcg ctattacgga gttcttccgg
     4321 actcagcccg cggaagtact cataagtccg gcgcaggcct tcagaacggt cgatcctggg
     4381 ctcccatccg agcacttccc gggcacgggt gatgtccggt tgacgttgtt tgggatcgtc
     4441 ctgtggaagc gggtgataga ctaccttctg attggttccg gtaaggcgga tgatctctt
//
LOCUS       DAOTON010000111         4498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15780_length_4498_cov_3.537025, whole genome shotgun sequence.
ACCESSION   DAOTON010000111 DAOTON010000000
VERSION     DAOTON010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15780_length_4498_cov_3.537025"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="P5292_03275"
     CDS             complement(<1..1209)
                     /locus_tag="P5292_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38178.1"
                     /translation="MNRFRRPDFRRRKAFTRFIYSFPIQLLVVMLKKNHLLLLYWVVL
                     FGWVTESLSKRFGIPYLFLDPEYMGTVGFLSFFIMGFATGAFIMVFNISSYIINGFRF
                     PFIATLSRPFLKYTINNFILPALFVLVYLGNLISFQLNNEYQGLWSIISEVTGFLIGM
                     TLIFTVTLTYFFRTNKDIVLMFGMETSDADPNAPVAAHVDDEDDLLLTRKLFTKKKKP
                     AQIKEWRVDTYLSTFRKVKLVRHTGHYTRDMVESVFRQNHLNAAIIEIIVFGMFILMG
                     LFKEYSLFKIPAGASVMLTFSLLIMLSSAFRFWLKSWAGSAFILLILVVNFLSQYGIF
                     YPENKAYGLDYTVKKSAYDMDAIRRSDDSLRVQADMAATIAQLEKWKEKAAVGGTKPK
                     MIFVNCSGGGL"
     gene            complement(1259..2917)
                     /gene="ftcD"
                     /locus_tag="P5292_03280"
     CDS             complement(1259..2917)
                     /gene="ftcD"
                     /locus_tag="P5292_03280"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRS38179.1"
                     /translation="MQQLIECVPNFSEGQDPAIIRQITEVIEATDGIRLLNVDPGKAT
                     NRTVVTFVGAPEQVVEAAFQAIRKAAELIDMSKHKGEHPRMGATDVCPLIPISGISME
                     ETAAWAQKLAQRVGNELKIPVYLYEAAQPDPARKNLSVIRAGEYEGFFEKIRKPEWKP
                     DFGPAVFPARSGATVIGARNFLVAYNINLNTTSVRRANSVAFDVRENGRKVKNEKGEE
                     IVQPGTCKSVKAIGWFIEEYGIAQVSMNLTDISVTPVHIAFDECVKSAYQRGLRVTGS
                     ELVGLIPLSAMTDAGRYFLKKQKRSTGVSEEELIRIAIKSMGLDELGPFKPEERIIEY
                     RLRDAAQERLVGMRLREFANETAAESPAPGGGSISAYVGALGVSLGSMVGNLSSHKKG
                     MDDRWEFFSDWAEKGQQLKDELLFLVDEDTRSFNRIMAAFGLPKATEEEKKARSVEIQ
                     AATRYATEIPLRVMKAAFASLAIIKAMAEEGNPNSVTDAGVGALCARTAVQGAYLNVR
                     INASGLKDKEFVDGILKEAAAVMEKAKIEEKVILETVERKITTA"
     gene            complement(2936..4204)
                     /gene="hutI"
                     /locus_tag="P5292_03285"
     CDS             complement(2936..4204)
                     /gene="hutI"
                     /locus_tag="P5292_03285"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRS38180.1"
                     /translation="MALLIRNIRTLVLAHPGSLSPLRGDAMDRIPLVDGAYLLVRHGK
                     FEAFGQETPEALAELRSRYTIGEEIDAQGGLVLPSWCDAHTHTVFAGSREQEFVDRMQ
                     GLSYQQIAERGGGILNSARRLRDASEDELFHAARERVLDAIRMGTGAIEIKSGYGLDE
                     ASELKMLRVIRRLRDHFPIPVRSTFLGAHAVPAEFQGDRSAYLKFLTDTLFPKVADEG
                     LADFCDIFCEQGYFTADETFQYLSKARAFGMRPKVHAEQLTRSGGILAGIKVGAISVD
                     HLEYANDEDIRALADSSTIPVLLPGAQLFLGLPAPPARAMITAGNAVALATDFNPGSS
                     PSSNMNLMVSLACILYKMTPREAICAATINAAAAMGIQDTHGSIGIGKVANFFVTSPL
                     ESVEAFPYFFGQPLIRQCFINGRTLSSLNI"
     gene            4182..>4498
                     /locus_tag="P5292_03290"
     CDS             4182..>4498
                     /locus_tag="P5292_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS38181.1"
                     /translation="MFRINKATGTLVDSPAKVAINPATACMDTYDVVILGAGPAGCTC
                     ALALHDAGLKVALVDKSDFPRDKVCGDAIGGRAERVLRELDPDLADEFSRRAFGVKAA
                     GWR"
BASE COUNT         1025 a         1216 c         1223 g         1034 t
ORIGIN      
        1 caagccgcca ccggaacaat tgacgaagat catcttcggt ttggtcccgc cgacggccgc
       61 tttctctttc catttttcca actgcgcgat cgtggcggcc atgtctgcct gaacgcgcag
      121 actgtcgtcg cttctccgga tggcatccat gtcgtacgcg ctctttttca ccgtgtaatc
      181 gagcccgtag gccttatttt ccggatagaa gatgccgtat tgcgatagga aattgaccac
      241 caggatcagg agaatgaacg cggagcccgc ccaggatttc aaccagaacc ggaaggcact
      301 gctcaacatg atcagcaggg agaaggtgag catcacgctg gcgccggccg ggatcttgaa
      361 aagggagtac tccttgaaaa gtcccatcag gatgaacatg ccaaagacga tgatctcgat
      421 gatcgcggcg ttcaggtgat tctggcggaa caccgattcg accatgtcgc gggtatagtg
      481 tccggtatgc cgtaccaact tgacttttcg gaaggtgctc aggtacgtat caacccgcca
      541 ctccttgatc tgggccggtt ttttcttctt ggtgaataat tttcttgtca gcagcagatc
      601 gtcttcgtca tccacatgcg ccgcgacagg cgcattggga tcggcatcgg aagtctccat
      661 gccgaacatg agcacgatgt ccttgttggt tcggaaaaaa tacgtcagcg tgacggtgaa
      721 gatcagcgtc atgccgatca ggaaaccggt cacctccgaa attatgctcc ataagccctg
      781 gtattcattg ttgagctgaa aactgatgag attgccgagg tacaccagga cgaacagcgc
      841 aggaagaatg aagttgttga tcgtgtactt cagaaacggg cgcgataagg tggcaatgaa
      901 cggaaagcgg aagccgttga tgatgtaact ggaaatgttg aacaccatga tgaacgcgcc
      961 ggtggcgaaa cccatgatga agaaactcag aaaaccgacg gtccccatgt actcgggatc
     1021 gagaaacaga tagggtatac cgaagcgttt ggaaagcgat tccgtcaccc agccgaacag
     1081 caccacccag tacaacaaca ggaggtgatt ctttttcagc atcaccacca gcaactggat
     1141 gggaaacgaa tagataaagc gggtaaaggc tttacgtctt cggaaatcgg ggcggcggaa
     1201 cctgttcatt gaacgcagta ccaataattt acgttcaaaa caggaaaaag tttgacactt
     1261 aagcggtagt tatcttacgt tcgaccgttt ccaggataac tttctcttcg attttagcct
     1321 tttccatgac tgccgccgct tccttcagga tcccgtcaac gaattccttg tcctttagcc
     1381 ccgaagcgtt gatccggacg ttcagatagg cgccctgtac ggctgtcctg gcacacaacg
     1441 cacctacacc agcatcggta accgaattgg ggttgccctc ttcggccatg gctttaatga
     1501 ttgcaagcga agcgaaggcg gccttcatga cacggagcgg tatttcggtc gcataccggg
     1561 tagccgcctg gatctccaca cttcgggcct ttttttcttc ctccgtagct tttggaagac
     1621 cgaaggcggc catgatacgg ttgaacgatc gggtgtcttc atcgaccagg aacaaaagct
     1681 cgtccttcag ttgctgaccc ttttctgccc aatccgagaa gaactcccaa cgatcatcca
     1741 tgcccttttt atgggaggaa agattcccga ccatggaacc cagggaaaca cccaatgcac
     1801 ccacataggc ggaaatggag ccaccgccgg gtgcagggga ttcagcggct gtctcgttcg
     1861 cgaattcgcg tagccgcatg ccgaccaaac gctcctgggc tgcgtcgcgc aaacggtatt
     1921 cgatgatccg ctcttccggt ttaaatggtc ccagttcgtc gagtcccatc gacttgatgg
     1981 cgatccgtat cagttcttct tccgatacac cggttgatcg cttctgcttt tttaaaaagt
     2041 accttcccgc atccgtcatt gcggataggg gaatcagacc gaccagttcg gaacccgtaa
     2101 cccgaagccc tcgttggtaa gcgcttttca cacattcgtc aaacgcgatg tgaaccggag
     2161 tcacgctgat gtcggtcagg ttcatgctga cctgggcaat gccgtattct tcgatgaacc
     2221 atccgatggc tttgaccgat ttgcaggtgc cgggctgtac aatttcttcg cctttttcat
     2281 tcttgacctt tcggccattt tcccgtacgt cgaacgcaac cgaattggcc cgtcgtacag
     2341 aagtggtatt gaggttgatg ttataagcca cgaggaaatt tcgtgctccg ataaccgtag
     2401 cgcctgaacg ggcaggaaaa acagcgggac cgaagtcggg tttccattcc ggcttcctga
     2461 tcttttcgaa gaagccctca tactcgccgg cgcggatgac cgatagattc ttcctggccg
     2521 ggtcaggctg agctgcctcg tagaggtata ccggaatttt caattcattt cccacgcgct
     2581 gagccagctt ctgcgcccag gcagccgtct cttccatgga gataccgctg atcgggataa
     2641 gcggacaaac atcggtagca cccatgcggg gatgctcacc tttgtgcttg ctcatgtcaa
     2701 tcagttccgc cgccttgcgg atggcctgga acgcggcttc cacaacttgt tcaggcgctc
     2761 cgacaaacgt aaccacagta cgattggtag cctttcccgg atcgacgttc agcaaacgga
     2821 ttccatcggt cgcttcgata acctcggtga tctgccggat gatcgccgga tcctgtcctt
     2881 cactgaaatt gggaacacat tcgataagct gttgcatatg gggtgttgtc agttgtcaga
     2941 tattgagtga ggataatgtc cggccgttga tgaagcactg ccggatgagt ggctgaccga
     3001 agaagtatgg aaatgcttcg acggattcaa gcggcgaggt cacaaagaaa ttggcaacct
     3061 ttccgatgcc gatacttcca tgtgtatcct gaatgcccat ggcggccgct gcattgattg
     3121 ttgcggcgca gatcgcttcc cgtggggtca tcttgtagag gatgcaggca agagacacca
     3181 tgaggttcat gttcgaactg ggactgctgc cgggattgaa atcggtcgcc agtgcgaccg
     3241 cattgccggc cgtgatcatc gcccgggccg ggggagcggg aagtccgagg aacaactgag
     3301 ctccgggcaa caggaccggt atggttgaag agtctgccag cgcccggatg tcttcgtcat
     3361 tcgcgtattc cagatgatcc acgctgatgg ctccgacttt gattccggcc aggataccac
     3421 cgcttcgggt caactgctcg gcatgcacct tgggccgcat tccgaacgca cgagcttttg
     3481 acaggtactg aaacgtctcg tccgccgtaa aatacccttg ttcacaaaag atgtcgcaga
     3541 aatcagcgag cccttcatcg gcgactttcg ggaataaggt atcggtaagg aatttcagat
     3601 aggctgatcg gtcaccctgg aactcggctg ggaccgcgtg tgctccaaga aaggtgctgc
     3661 gcaccggaat cgggaagtga tcccgcaacc gacgaatgac ccggagcatt ttcaactcgc
     3721 tggcttcgtc aaggccataa ccgctcttga tctcgatcgc tccggttccc atccggatcg
     3781 catccagcac acgctcacgt gccgcgtgga aaagctcatc ttccgaagca tcccgaaggc
     3841 gcctggctga attcaatata ccacccccac gctccgctat ctgctgatag ctgaggccct
     3901 gcatacgatc aacgaattcc tgttcacgac taccggcaaa tacggtatgc gtatgggcat
     3961 cgcaccagga gggtagcacc aatccgcctt gggcatcgat ctcttcgccg atcgtgtagc
     4021 gggatcgaag ttcggccagg gcctccggtg tttcctgtcc aaaggcttca aatttcccgt
     4081 gccggacaag caggtaggct ccatccacga gcggaatacg gtccatggca tcgccacgaa
     4141 gcggagaaag tgaacccggg tgggcaagca ccagggtacg gatgttccgg atcaataaag
     4201 ccacaggaac tttggtagat tcgccggcga aagtagcaat taatcccgcc accgcctgta
     4261 tggataccta cgatgtcgtg atccttggcg ccggtccagc cggttgtacc tgtgctttgg
     4321 ctttgcatga cgcagggctg aaggtggcgc ttgttgacaa aagtgatttt ccgcgcgaca
     4381 aggtatgtgg ggatgcgatt ggagggaggg ctgaacgtgt cctgagggaa cttgatccgg
     4441 acctggccga tgaattttca aggcgcgcgt tcggcgtaaa ggctgccggc tggcgatt
//
LOCUS       DAOTON010000112         4496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15792_length_4496_cov_3.506643, whole genome shotgun sequence.
ACCESSION   DAOTON010000112 DAOTON010000000
VERSION     DAOTON010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15792_length_4496_cov_3.506643"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="P5292_03295"
     CDS             <1..912
                     /locus_tag="P5292_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS38182.1"
                     /translation="KNENFDALFSPALSAVYTMKPNQILRVSFSSAIRNPTLADQFLY
                     YNVGRAVLLGNTKGYDSLVTVPSLLDYFINQTPLEYFNLDPVKPEKVRTVELGYRGTI
                     GKNLFVDLVGYYSSYTDFIGYQVGADVTVIPSASLIYINNIYRIAANASESVSTYGTS
                     IGLSYFLGKFLTINGNYSFNRLEKSSSDPLIPAYNTPENKYNIGISGRDIEAEVLGLR
                     LRNYGFSINYKWVEGFRFEGSPQFTGYVPQYDFVDAQISKRVPKIHATFKVGASNVLN
                     QRRFTVYGGPETGRLAYASILVELQKN"
     gene            979..2514
                     /locus_tag="P5292_03300"
     CDS             979..2514
                     /locus_tag="P5292_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38183.1"
                     /translation="MKKQLLLILCAVSLWQFASAQRYSTEIFSDVSVQQNIVYGDNFS
                     VLTGTPTSVQLKMDVYTPVGDVETSRPVVVMLHTGSFLPAIINQTPTGSMRDSAIVEM
                     CSRFARRGYVAISADYRTGWNPQAVGSAGQDIRTGSLLQAVYRSILDIKACVRYIKND
                     VATANTYGIDTTKIILGGMGSGGYIALAYATIQDTADFNLPKFISSTTNASYGFVAGS
                     SYVNTGLWGDIEGYGGTPGLNNPNNTPGRSTDVAFVFNCGGALGDSSWLRAGDVPMVC
                     FHPVTDPFAPYGDGTVIVPTTGDPVVDVSGSQTIVTRANALGNNDCFLSATFTDPFTA
                     AADLNNNGQDGLFPFLFATPQAGPWEWFDSTTTVQVAIALGLGQAAGTQIYQNAKLTN
                     PDMSKAKGMAYIDTIQGYVNPRMIQCLGLNVGITDLGQLNQQVSVYPNPSAGAFTVDL
                     RQVADAVSYIRVADLTGQIVRDLRPDLSGLNPVDAAGLSKGVYTVRVGFSNGEVVRKV
                     VLQ"
     gene            2599..3858
                     /locus_tag="P5292_03305"
     CDS             2599..3858
                     /locus_tag="P5292_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38184.1"
                     /translation="MLRSLLLFAFGISLAWNASAQTIIDCGSGRYDSELFPDVTVQSD
                     VIYGSNVDANGQTLQLKMDIYQPDGDTAAYRPAIVMVHGGSFIAGSKLDNDVVEICTR
                     FAKRGYVCASIDYRIGIPFPIAEANAVRAVYRAVQDMKAAVRFFRQDAATANTYRIDP
                     DIIFGGGSSAGAFTALHLAYLDDPAELPSQIDTVALGGMEGNSGNPGYSSEIKAVVNL
                     CGALGNKTWIHPGDEPLCSMHGTVDNTVPYGTDMIYLAGIFPIMIVDGSYPVAEQGNL
                     VGLDNTMYTFFGADHVPYAIDAAYMDTTIRFVSNFLYRQLGCTPADPEPLANTFPTTG
                     IQDQQKADWSIFPNPAAGRFQWQNLPAEAASFRLVDPQGRVVINSREFRDRAVDLSGL
                     PAGMYFIRLMNEKEQEIGQKVLILLGK"
     gene            complement(3950..>4496)
                     /locus_tag="P5292_03310"
     CDS             complement(3950..>4496)
                     /locus_tag="P5292_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRS38185.1"
                     /translation="EVAGQLSVEKIIRKIKLMHQSLRRDFGFRKPRIAVMGLNPHAGD
                     NGLIGQEEQQVIIPAIKQCFDEGMFVYGPYSADGFFGSGAFQKFDAVLAMYHDQGLVP
                     FKTLSFGTGVNYTAGLPFVRTSPDHGTAYDIAGKGEANEESLRDAIYTAIDVLRKRAE
                     YDEINANPLAFGKQTKEGRGD"
BASE COUNT          947 a         1299 c         1172 g         1078 t
ORIGIN      
        1 aagaacgaga atttcgatgc gctcttttca ccggctcttt cggcggttta taccatgaag
       61 cctaaccaga tccttcgcgt atcgttttcc tccgctatcc ggaatcccac actcgctgac
      121 caattcctct attacaatgt cggtcgtgcg gtcctgttgg gcaatacgaa agggtacgat
      181 tctctggtga cggtcccgtc cctgctcgat tacttcatca accaaacccc cttggagtat
      241 ttcaatctcg atccggtcaa gcccgagaaa gtgcgtactg tagaactcgg ctaccgggga
      301 acgatcggga agaacctctt cgtcgacctg gtcggttatt acagcagcta caccgacttt
      361 atcgggtatc aggttggtgc cgatgtaacg gtgatcccga gcgcttcgct catttatatc
      421 aataacatct accggatcgc cgccaacgca tcggaatcgg tatcgaccta tggtacctcc
      481 attggcctga gctattttct gggaaagttt ctgacgatca acggcaacta ttccttcaac
      541 cgtttggaaa agagttcatc cgatccgttg atcccggcat acaatacgcc ggaaaacaag
      601 tacaacatcg ggatctcggg tcgtgatatc gaggctgagg tgcttggact tcgactccgg
      661 aattacggct tcagcattaa ctataagtgg gtggaaggat tccgctttga gggctctccc
      721 cagttcaccg gctatgtgcc gcaatatgat tttgtcgatg cccagatctc caagcgggtg
      781 ccgaaaattc acgcgacctt caaggtgggc gcttccaatg tgctgaatca gcgtaggttt
      841 acggtttacg gcggaccgga aacgggtcgc ctggcctatg cttccatttt agtggagctt
      901 cagaaaaatt gaccgtggcc attccgttcc ggaatcggaa tttccgtaaa ttgcatttcc
      961 tatcttaacc tgactcggat gaaaaaacaa ctacttctga tcttgtgtgc ggtttccctc
     1021 tggcagttcg cgtctgcgca acgctactca acggaaatct tttccgatgt ttctgtacag
     1081 cagaacattg tttatggtga caacttcagt gttctcaccg gcacacctac cagcgttcag
     1141 ctgaaaatgg acgtgtatac tcctgttgga gacgtcgaaa ccagccgtcc ggtcgtggtc
     1201 atgctccaca ccggcagctt cctgccggcg atcatcaacc agacgcctac cggttcgatg
     1261 cgcgacagcg ccatcgtgga aatgtgcagc cgcttcgcac gccgcggcta tgtggccatc
     1321 tccgccgact accgtaccgg ctggaacccg caggccgtcg gttctgccgg acaggatatc
     1381 cgtaccggta gcctgctcca ggctgtttac cggagtatcc tcgatatcaa ggcttgtgtc
     1441 cgttacatca agaatgatgt ggcgaccgcc aacacctacg gtatcgatac caccaagatc
     1501 atcctcggcg gtatgggctc cggcggttat atcgcgcttg cctatgctac cattcaggat
     1561 acggccgact tcaacctgcc gaagttcatc agcagcacta cgaatgcgtc ctatggcttc
     1621 gtcgcgggtt ccagctatgt gaacaccggc ctctggggtg atatcgaagg ctacggcgga
     1681 acgcccggcc tcaataaccc gaacaataca cccggtcgtt cgaccgatgt tgcattcgtc
     1741 ttcaattgcg gcggcgcgct cggcgacagc tcctggctgc gcgccggtga cgtaccgatg
     1801 gtttgtttcc atccggtaac cgatccgttc gctccttatg gagacggtac ggttatcgtt
     1861 ccgaccaccg gcgacccggt tgtcgacgta agcggtagcc agaccatcgt tacacgtgcc
     1921 aatgccttgg gtaacaacga ttgcttcctt tccgctacgt tcaccgatcc tttcacggcg
     1981 gctgccgacc tgaacaacaa cggtcaggat ggactgttcc ccttcctctt cgccacgccg
     2041 caggctggtc cctgggagtg gttcgattca accactactg ttcaggttgc catcgccctc
     2101 ggtctcggac aagctgccgg aacgcagatc tatcagaacg ccaagctgac caacccggat
     2161 atgtcgaaag ccaaaggaat ggcctacatc gacaccattc agggttacgt caacccgcgt
     2221 atgatccagt gtctcggcct caacgttggc attaccgatc tgggtcaatt gaatcagcag
     2281 gtttcggttt acccgaatcc ttcggccggt gctttcaccg tcgaccttcg tcaggttgcc
     2341 gacgctgttt cgtacatccg cgtggctgat ctgaccggac aaatcgtccg cgatcttcgt
     2401 ccggatctca gcggtctcaa cccggtcgat gccgccggac tttcaaaagg tgtctacacc
     2461 gtacgcgtcg gcttctccaa cggcgaagtc gtgcgcaaag tggtgctgca ataattacta
     2521 cttgaagcga gatgattgga aggccacccc gaacgggtgg ccttcttttt ttgtattggt
     2581 aattcattac cttcatgcat gcttcgatca ctactgttat tcgcattcgg tatttccctg
     2641 gcctggaacg ccagcgccca aacgatcatc gattgcggct ccggccgtta cgattccgag
     2701 ctgttcccgg acgtgacggt tcaatccgat gtgatttacg gttccaacgt tgacgccaat
     2761 ggtcagacgc ttcagttgaa gatggacatc taccaaccgg acggtgatac tgcggcctac
     2821 cggccggcca tcgtcatggt ccatggcggt agtttcattg ccgggtcgaa actcgacaac
     2881 gacgttgtgg agatctgtac ccgtttcgct aaacgagggt atgtctgtgc ttccatcgac
     2941 taccgaatcg gtattccgtt tccgatcgcg gaagcgaacg cggtacgcgc cgtgtatcgg
     3001 gcggtacagg atatgaaggc ggctgtgcgc ttctttcggc aggatgccgc cacagcgaat
     3061 acctatcgga tcgatcccga tatcatcttc ggcgggggga gctccgccgg cgctttcacc
     3121 gcgttgcacc tcgcttacct cgatgatccc gcggaactgc cgtctcagat cgacaccgtc
     3181 gcgctcggcg gtatggaagg taacagcggt aatcccgggt attcatcgga gatcaaagcc
     3241 gtcgtgaatc tttgtggcgc gttgggtaat aagacgtgga ttcatcccgg cgatgaaccg
     3301 ctctgcagca tgcacggaac ggtggacaat accgtaccct atggtaccga catgatctac
     3361 ctcgccggga ttttcccgat catgatcgtg gatggaagct atccggttgc cgaacaaggg
     3421 aacctcgtcg gactcgacaa taccatgtac acgttctttg gtgccgatca cgtaccctat
     3481 gcgatcgatg ccgcatacat ggacacaacc attcgcttcg tcagcaattt cctctatcgc
     3541 caactcggct gcacgcctgc cgacccggaa ccgctcgcga atacgtttcc gactaccggt
     3601 attcaggatc agcaaaaagc ggactggagc atctttccga atccggcagc aggccgtttc
     3661 caatggcaga accttccggc agaagccgct tcctttcgtt tggtcgatcc gcaaggacgt
     3721 gttgtcatta actcccgcga attccgtgat cgggccgtcg acctgtccgg tctgcccgcc
     3781 gggatgtatt tcattcggct gatgaacgag aaagagcagg aaataggtca gaaagtgctg
     3841 atactgctgg gcaaataacc ggcccgccca actcgaaact tggaactcgg aacacggaac
     3901 ctgaaacccg cctgcctgcc tttcgaagct ttagcgtagg caggaaacct caatctcctc
     3961 ttccttcttt cgtctgcttt ccgaacgcaa gcgggttggc attgatctcg tcgtactcgg
     4021 cccgtttgcg cagtacgtcg atggccgtat agatagcatc gcgaagtgac tcttcgttgg
     4081 cttcaccttt tccggcaatg tcgtaagcgg tgccatgatc cggtgaggtg cgaacgaacg
     4141 ggagtccggc cgtgtaattg accccggtac cgaagctcaa cgtcttgaaa ggaaccaatc
     4201 cctggtcatg gtacatggcc aagacggcgt cgaacttttg aaaagcgccg gaaccgaaga
     4261 atccgtcggc agagtagggg ccatatacga acatgccttc atcgaaacac tgcttgatgg
     4321 ccgggatgat cacctgctgc tcctcttgcc cgatgagtcc gttgtcgcca gcatgcggat
     4381 tgagccccat gacggcaatg cgcggcttgc gaaatccgaa gtcgcggcgg agcgattggt
     4441 gcatcagctt gatcttgcgg ataatctttt ccacactcag ttggcctgcg acttcc
//
LOCUS       DAOTON010000113         4490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15817_length_4490_cov_2.777678, whole genome shotgun sequence.
ACCESSION   DAOTON010000113 DAOTON010000000
VERSION     DAOTON010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15817_length_4490_cov_2.777678"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2875
                     /locus_tag="P5292_03315"
     CDS             <1..2875
                     /locus_tag="P5292_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38186.1"
                     /translation="LQASAVAGLVFLFFSVIGADMRACLHLLAAFQGAVVVSVISRHL
                     MKAQVNDEINLLVIGWLGALGISLGILIRTFFSGSLPLPSLPESVFVTVCVGWVFLFY
                     QLKRPQHPTNHALGLIRRSSGLVVAPLLIVVAAELRMIFGMESQLPGWIALAMVAASV
                     FRPHRHPAELHLVQERFFSRTLPLFIAGVLAYAYYQPILAAQVDVFEDANRILPLLQW
                     HQFGKIPFLDSFSSHALSDWCWGGLYNLLNGFDAYGTYLYGFLLHVLIAATTYWFVWK
                     IWNNGWLALFLALFYPYTHLLIPAYYHFVPLTVVLLLPIANQPTRKHFILLFSWVFFL
                     MLWRLDVGLSNTLATIALVLILGFSGEPKPFFRTALRGLLPAALPWLLLGLAALALRG
                     GAIFSRLREILAYTNSYQSFGLRTLGTENGLIAQLLYYILPAAVLIVLGVAVLRPRRH
                     RPTVLAMAFFTAFFLVNLQRGLIRHSLAEGWDAAWTSYGFFVLAASVYVSGKTRSITR
                     FFSFSIAATLIVLLLKYDRPAADTNNRYAQVSARSFQAINLFPQPGTRVQLSADQTAF
                     ANELKHVLEHDFSDSATFLDFSNTPMLYAFTGRAVPNYFCQIPHTAHNSFLQERFLDE
                     LKNYDIPLVVFAHQPENFWDALDGIPNAARHYRIAEYIYAHYRPAWLLGPHTVWLRND
                     LTIPATKEVTRLWRQDWTHCRVTQGYLADSITVRSHGDGLRIVDILPADGIALTSGKR
                     YYLRVEGHAEGGGNAELFIKATGLEKPPPVRWNLQWGATTAYFPITLPDSCTRLEQLE
                     LQLPVQVFRLQSLALVEADHVPDQFSGAFRVWDLGSIPYYWGTYDQGVITQSLELLNN
                     RSLIADAEVRFSLPKPGVKATYLDLDAQIAGDQPVNMIVSYGKGETKLGAFTLRISPG
                     AEARYRIRALSQYNWFAAQPDWVALYPAGKDVQLRRFSLANGE"
     gene            2880..4139
                     /locus_tag="P5292_03320"
     CDS             2880..4139
                     /locus_tag="P5292_03320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRS38187.1"
                     /translation="MIRTRTKVVLDAVAGRTLVFFLNLAARVLGRILSIDHALTRPPK
                     TIVVCKYLGMGSIIQATPLLQTLRKNFPDARLVFVTSQANRGLMKQVAAVHEVLTVND
                     SGLLPLASSSWKLLRRLWKLRPDLYIDLETYSYYSTAIATMSCARNRIGFYRVERNIR
                     MGVYTHMMFFNARAPIAQSYLQMARLVGCREIVAQLYPFRVDEADLASMRRKLERQVG
                     ESFEPFVVINPNASDLRIERRWPSERFIALIRSLSQRFPSHRFFLIGSVSEAAYVGEI
                     YRRLELPNEDQVINTAGRLSLGELFALISRSELVVTNDTGPMHIAFSLGRPTVSLFGP
                     ASPSQYGDNPHAFGIYKNLYCSPCVHDFLVPPCGGDNQCMKQITLEEVEGLCTRLLRG
                     DRSTVPAENLRFNRAGADGALGVVNRL"
     gene            4136..>4490
                     /locus_tag="P5292_03325"
     CDS             4136..>4490
                     /locus_tag="P5292_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38188.1"
                     /translation="MSQAPVTCRCCGSQQQHFLVGGLDSIYARRTYDVVVCEQCSNGI
                     TVPAETQASLDRLYAETYLYPVHECVIGEKRYRAEGLARFLRQHYPAAKYSNVLEIGC
                     MFGYLMEALRRDYRVK"
BASE COUNT          851 a         1306 c         1274 g         1059 t
ORIGIN      
        1 cttgcaggcg agtgccgtag cgggtctggt ctttctcttc ttctcggtga ttggagccga
       61 tatgcgcgcg tgcctgcatc tgcttgctgc atttcaagga gcggtcgttg tctccgtcat
      121 cagccgtcac ctgatgaagg cacaggtgaa cgatgagatc aatctcctgg tcatcggctg
      181 gctgggagcc ctgggaatca gcctcggcat cctgatccgc accttctttt cgggaagcct
      241 gccactcccc tccctgccgg agtcggtctt tgtgacggtt tgcgtgggct gggtatttct
      301 gttctatcag ttaaagcgac cgcaacaccc gacgaatcac gcgctgggtc tgatccgacg
      361 cagcagcggt ctcgtcgtcg ccccgctgtt gatcgtggtg gctgctgagc tgcgcatgat
      421 cttcggaatg gaatcgcaac tgcctggctg gatcgcgctg gcgatggttg cagcatccgt
      481 ttttcggccg catcgacatc ctgccgaatt gcatttggtt caggaacgct ttttcagccg
      541 tacgctcccg ttgttcatcg ccggcgtact tgcctatgcc tactatcaac cgatccttgc
      601 cgcgcaggtg gatgtattcg aggacgcgaa ccgcatactg cccttgctgc aatggcatca
      661 gttcggaaag attccgttcc tggattcctt cagctcgcac gcgctctcgg actggtgctg
      721 gggcggcctg tacaatctgt tgaacggatt cgacgcttac ggaacctacc tctatggctt
      781 cctgctgcac gtcctgatcg cggcgaccac ctattggttt gtctggaaga tctggaacaa
      841 cggctggctg gcactgttcc tcgccctgtt ttatccatac acgcacttgc tgatcccggc
      901 gtattatcat tttgtgccgt tgacggtggt gctcttgtta ccaatcgcga atcaacctac
      961 gcgaaagcac tttatcctgc tcttcagttg ggtgtttttc ctgatgctct ggcgactgga
     1021 cgtcggatta tcgaataccc tggcgactat cgcgctggtg ctaattctcg gattttccgg
     1081 ggagccgaag cccttcttcc gtaccgcgct acgcgggttg ctgccagcgg cattgccctg
     1141 gttgttactc gggctggcag cgctggcgct gcgcggtgga gcgatcttct caaggctgag
     1201 agagatcctg gcttatacca attcctatca atcgtttgga ctgcgaacgc tcggcaccga
     1261 gaacggattg atcgcgcagt tgctgtatta catacttccc gccgccgtgc tcatcgtcct
     1321 cggtgttgcg gtattacgtc cgcgccggca tcgcccgacc gtgttggcga tggcgttctt
     1381 cacggccttc ttcctcgtca acctgcaacg cggactgatc cgccacagcc tggcggaagg
     1441 ttgggatgcc gcctggacct cttacgggtt cttcgtgctg gcggcgagcg tctatgtaag
     1501 cggcaaaacc cgcagcatca cgcgcttctt ttccttcagc attgcagcaa ccctgatcgt
     1561 cctgttgttg aaatacgatc gtccggcagc ggatacgaat aaccggtacg ctcaggtgtc
     1621 ggctcgcagt ttccaggcga tcaatttgtt tccgcaaccc ggcacacgcg tacagttgtc
     1681 ggccgaccaa accgcgttcg cgaacgagct gaaacatgta cttgaacatg acttcagcga
     1741 cagcgccacc ttcctcgatt tttcgaatac cccgatgctg tatgcgttca cgggccgggc
     1801 ggtgccgaat tatttctgcc agatcccgca taccgcgcac aacagctttt tacaggagcg
     1861 gttcctggat gagttgaaga actatgacat tccgctggtc gtgttcgcgc atcagccgga
     1921 aaacttctgg gacgcgctcg acggcattcc gaacgcggcg cgtcactatc ggatcgcgga
     1981 gtacatctat gcccattatc gtccggcctg gctgctcgga ccacacaccg tttggttgcg
     2041 taacgacttg accataccgg cgacaaaaga agttacccga ctctggcggc aagactggac
     2101 gcactgccgg gtcacgcagg gctatctggc cgactcgatc acggttcgca gccatggcga
     2161 tggtctccgc atcgtggaca tactgccggc ggacggtatc gcactcacat cggggaagcg
     2221 gtactattta cgagtcgaag gacatgcaga aggcggcgga aacgccgagt tattcataaa
     2281 agcaacgggg cttgaaaaac ctccaccggt caggtggaat cttcagtggg gtgcaacgac
     2341 tgcttacttc ccgatcacat tacccgacag ttgtacccgg ttggaacaac tcgaacttca
     2401 gttaccggtt caggtctttc gattgcaatc gctggcgctg gtggaagcgg atcatgtgcc
     2461 ggatcaattc tccggagcct ttcgtgtttg ggacctggga tccatccctt attactgggg
     2521 aacctacgac cagggtgtga tcacacaaag tcttgaactg ctgaacaacc gaagcctgat
     2581 tgcggacgcg gaagtccgct tctcgttgcc gaagccaggg gtaaaagcta cgtacctcga
     2641 tcttgatgca cagatcgctg gtgatcagcc ggtgaacatg atcgtcagtt atggcaaagg
     2701 ggaaacgaaa ttaggtgcct tcacactccg aatctctccc ggcgcggaag cccggtatcg
     2761 aatcagagcc ctgagccagt ataactggtt tgccgctcag ccggattggg tagctttgta
     2821 cccggccggc aaggacgttc aactgcgacg tttttcgctc gccaacggcg aataaaccca
     2881 tgatccgtac ccgtaccaaa gtcgtgcttg atgcggtggc gggaagaacg ctggtgttct
     2941 tcctgaacct cgccgcgcgc gtactcgggc ggatcctctc catcgatcac gcactcaccc
     3001 gaccgcccaa aacgatcgtg gtctgcaaat acctcggcat gggcagcatc atccaggcca
     3061 caccgctctt gcagaccctg cggaagaact ttcccgatgc caggttggtc ttcgtcactt
     3121 cacaggctaa tcgcgggctc atgaagcagg ttgccgcggt acacgaagta ctgacggtga
     3181 atgactccgg tttgctgccg ttggccagtt cgagttggaa actgttgcgg cggctctgga
     3241 aacttcgacc ggacctgtac atcgatctcg aaacctactc gtattacagc accgccatcg
     3301 cgaccatgag ctgcgcgcgt aatcgtatcg gcttctatcg tgtggagcgg aacatccgca
     3361 tgggggttta tacccacatg atgtttttca atgcccgggc accgatcgcg cagtcgtatt
     3421 tgcagatggc acgtctggtc ggctgccggg agatcgtcgc gcagttgtat ccgttccgcg
     3481 tggatgaggc cgatcttgcc tcgatgcgtc ggaagctgga gcggcaagtc ggtgaatcgt
     3541 tcgagccgtt cgtggtgatc aatccgaacg cgtccgacct ccgcatcgaa cggcgctggc
     3601 cctcggagcg gtttatcgcc ctgatccgtt cgctgagtca gcggttccct tcccatcgct
     3661 tctttctgat cggttccgtg agtgaggccg cgtatgtcgg agagatctac cgtcggctgg
     3721 aactgccgaa cgaggaccag gtgatcaata cggcagggcg actgagcctc ggagagctgt
     3781 tcgcgctcat cagcagaagc gaattggtcg tcacgaacga caccggaccg atgcacatcg
     3841 cgttttcgct cggccgcccg actgtttcgc tgttcgggcc ggcttctcct tcacaatacg
     3901 gcgacaaccc acacgccttc ggtatctata agaacctgta ttgcagtccg tgcgtgcacg
     3961 acttcctcgt cccgccctgc ggcggcgata accagtgcat gaaacagatc acgctcgaag
     4021 aagtggaggg tctctgcacg cgtttgctgc gtggcgaccg cagcactgtt ccggctgaaa
     4081 acttacgctt taaccgcgcc ggggccgacg gcgccttggg tgtcgtgaac cgtctatgag
     4141 ccaggctcca gtcacgtgtc gctgttgcgg cagtcagcaa caacatttcc tggtcggcgg
     4201 gctcgactcg atttatgcgc gtcgtacgta tgatgtcgtg gtgtgcgaac agtgcagtaa
     4261 cgggatcacg gtgccggcag aaacacaggc atcactggat cgcttgtatg cggagaccta
     4321 cctgtatccc gttcacgaat gtgtgatcgg ggaaaaacgc tatcgggccg aaggcctcgc
     4381 acgcttcctg cgccagcact atcctgcggc gaagtattcg aacgtactgg agatcggttg
     4441 catgttcggt tacctgatgg aagcgcttcg tcgtgattat cgcgtcaagg
//
LOCUS       DAOTON010000114         4485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15840_length_4485_cov_3.744921, whole genome shotgun sequence.
ACCESSION   DAOTON010000114 DAOTON010000000
VERSION     DAOTON010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15840_length_4485_cov_3.744921"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="P5292_03330"
     CDS             complement(<1..200)
                     /locus_tag="P5292_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38189.1"
                     /translation="MSQGVISPAIGGFFFKSRDPKSLARWMEDRLGVPFGTQLYASFL
                     WKTSPDDSMTGRAVARATEGRI"
     gene            complement(200..1603)
                     /locus_tag="P5292_03335"
     CDS             complement(200..1603)
                     /locus_tag="P5292_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HRS38190.1"
                     /translation="MKNTYRDLIEQTFYFPQEGFEVIDNQLHFYGIDLMEIIKQYGTP
                     LKISYLPKIGSQIKKAKRIFNVAIAKADYKGSYTYCYCTKSSHFSFVLDEVLKNEVHL
                     ETSSAYDIQIIRSLYAAKKLDKSITIICNGFKRDQYIQYICDLINDGFENVIPVLDNM
                     NELEQYEKRVKGRRKVKFGIRIAAEEEPNFEFYTSRLGIRYDEIKDYYVKSIKKNKRF
                     QLKMLHFFINTGIKDTAYYWSELNKCVNVYGELKKVCPTLDSLDIGGGWPIRHSLGFE
                     YDYEYMAEQIIKSIQSACKTQGVDEPNIYSEFGSFTVAESGATLYSVVDKKQQNDSEL
                     WYMIDSSFITTLPDTWGIKQKFILLAINQWERDYDRVNIGGITCDSDDYYNNESHVNQ
                     VYLPRIREDDKEPLYMGFFHTGAYQESIGGYGGIQHCLIPAPKHVIIDRDQDGEIVTR
                     LFAKEQSAKSMLKILGY"
     gene            1744..2685
                     /gene="rocF"
                     /locus_tag="P5292_03340"
     CDS             1744..2685
                     /gene="rocF"
                     /locus_tag="P5292_03340"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HRS38191.1"
                     /translation="MKKIRFIEVKSEIAAGTRGASMGPDAIKVAALDYGSNLFKKIDA
                     TEVPNENKQLFDAPGSPYAKRIKAVYAMLERVANAVKNSLKGNEFPIVLAGDHSTAAG
                     TISGIRMAYPKQRLGVIWVDAHADLHSPWTTPSGNMHGMPLAAVLNEDNASNKMNRPD
                     KETLEWWNKIKKLGNIAPKLDYKDLVFIALRDTEPEETYLLKKHKVKVFSTSEVKRNG
                     VEKIARDTLAHLNHCHLIYISFDVDSMDSSISKGTGTPVRNGITEKEAGSLLVRLIQN
                     EKVCCFEIAEVNPTLDKENLMAENTFEILQRVVSQLA"
     gene            2701..>4485
                     /gene="argS"
                     /locus_tag="P5292_03345"
     CDS             2701..>4485
                     /gene="argS"
                     /locus_tag="P5292_03345"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRS38192.1"
                     /translation="MSIQRFQDTIRQSVVNAVQDRFGKSIAPDQVVFQETKREFEGDI
                     TLLVFPLTKVSGLSPELTAEQLGGYLVENSGIVTGFNVVKGFLNLVIADRFWNDFFQA
                     IAADTRQLFDAGKTARRRLMVEYSSPNTNKPLHLGHIRNNLLGYSVAEILKAAGHDVT
                     KVNLVNDRGVHICKSMLAWKKFGHGETPESSGMKGDHLVGKYYVVYDKKLKEQIEEGK
                     KAGLPEEAAEKQAPLTLEVQEMLRQWEAGDPETIALWKRMNGWVYEGFDETYRLMGVD
                     FDKTYYESETYLLGKRIVDEGLSQGVFYRKDDGSVWIDLTDVGLDQKLLLRSDGTSVY
                     ITQDLGTAQLRYDEFKGLESLIYVVGNEQEYHFKVLREIFRKLGREWATGLYHLSYGM
                     VDLPSGKMKSREGTVVDADDLIREIVEQCEMATKAQGKIDDFDSAEARHLYHTLGMGA
                     LKYFILKVDPKKRMLFNPAESIDINGNTGPFIQYAHARICSVLRKGSAANLEKGLAGT
                     HRPDEGEKELLRVLYRYPEVLQDAANGYSPALIANYAYELAKSFNHFWHDHQIIDAAN
                     PGASAFRLELSRVTGSVIKEALRLLGIEA"
BASE COUNT         1080 a         1124 c         1223 g         1058 t
ORIGIN      
        1 tcgatcctcc cctcggtggc gcgggcgacg gcccggccgg tcatcgagtc gtcaggagac
       61 gtcttccata ggaaagatgc atacaactgc gtgccgaacg gaacacccag acggtcttcc
      121 atccatcgag ccagtgattt tggatcccgg gatttgaaga agaagccgcc aatggcaggg
      181 gagatgacgc cctgactcat caatagccga ggattttgag catgctcttc gcgctttgct
      241 cttttgcgaa caatcgggta acgatctctc cgtcctgatc ccggtcgatg atgacatgct
      301 tgggtgccgg aatgagacaa tgctggatgc cgccgtatcc gccgatgctt tcctgataag
      361 cgccggtatg aaaaaaaccc atatacagcg gctctttgtc gtcttcccgt atgcgcggaa
      421 gatacacctg gttgacgtgg gattcgttgt tgtaataatc gtcactgtcg caagtgatac
      481 cgccgatatt cacccggtcg taatcccttt cccattggtt gatcgccagc aggatgaatt
      541 tttgcttgat gccccaggtg tcgggcaggg tagtgatgaa ggaactgtcg atcatgtacc
      601 acagttcact gtcattctgc tgtttcttgt cgacgactga gtagagcgta gccccgcttt
      661 cagcaacggt gaagcttccg aactccgaat agatgttggg ttcgtcgacg ccctgggtct
      721 tgcaggccga ttggatcgat ttgatgatct gctcggccat gtactcgtaa tcgtactcaa
      781 agcccaggga atgtcgaatg ggccatccgc caccgatgtc gagcgagtcg agcgtcgggc
      841 agactttttt caattcgcca tagacattga cgcacttgtt caactccgac cagtaatagg
      901 cggtatcctt gatgcccgtg ttaatgaaga agtgcagcat cttcaattga aaacgcttgt
      961 tcttcttgat cgatttgacg tagtaatcct tgatctcgtc gtaccggatg ccgaggcgcg
     1021 aggtatagaa ttcgaaattg ggttcctctt cggccgcgat ccggattccg aacttgacct
     1081 tcctgcgccc cttgacccgt ttctcgtatt gctccagctc gttcatgttg tcgagcacgg
     1141 gaatgacgtt ttcgaatccg tcgttgatca ggtcgcagat gtactgaatg tactggtctc
     1201 gtttaaaacc attacaaatg atcgtgatgg acttatcgag tttctttgcc gcgtacaacg
     1261 accggatgat ctggatatcg taggccgagg aggtctccag gtgtacttcg ttctttaaaa
     1321 cctcgtccag tacgaaactg aaatggctgc ttttcgtaca gtaacagtac gtatacgacc
     1381 ctttgtagtc ggctttggca atggctacgt tgaaaatccg cttcgctttc ttaatttgcg
     1441 atccgatttt ggggaggtag gagatcttca acggagtacc gtattgcttg atgatctcca
     1501 tcaaatcgat gccgtagaag tgtagctggt tgtcgatgac ctcgaatcct tcctggggga
     1561 agtagaacgt ttgttcgatc aggtcgcgat aggtgttttt catcgtcccg tggatgctcc
     1621 gggtaagtcg gattggtcgt tttagcgtga agtaagtttc ccggatcgcc gggggcttgc
     1681 gaaagtaagt aacttacatt tccctcctgc gttggagagt aaatttttag atgccagaac
     1741 cttatgaaga agatccggtt cattgaagtc aagtcggaga tcgccgccgg tacccgggga
     1801 gccagcatgg gaccagatgc catcaaagtg gcagcgctcg actacggaag taacctgttc
     1861 aagaagatcg atgcgacgga agtgccgaac gagaacaaac agttgttcga tgctccggga
     1921 agtccgtacg cgaagcgtat caaggcggtt tatgccatgt tggaacgggt ggccaacgcg
     1981 gtgaagaatt ccctcaaggg taatgagttt ccgatcgtcc tggctggcga ccattcaaca
     2041 gcggcgggaa cgatctccgg tatccggatg gcttacccga agcagcgcct cggcgtgatc
     2101 tgggtcgacg cccatgccga tctccattca ccctggacga ccccgtccgg caacatgcac
     2161 ggcatgccgt tggctgcagt actcaacgag gacaatgcct cgaataagat gaaccggcct
     2221 gataaggaga cactggagtg gtggaacaag atcaagaaat tgggaaatat tgcacccaaa
     2281 ttagactata aagaccttgt attcattgcc ttacgggaca ccgaaccgga agagacctat
     2341 ctgctgaaaa aacacaaagt gaaggtcttc agcacgagcg aagtgaagcg caatggcgtg
     2401 gaaaagatcg cccgcgatac cctggcacac ctgaaccact gccacctgat ctatatcagc
     2461 tttgacgtgg attccatgga ttcctccatt tcaaaaggaa ccgggacccc cgtccgtaac
     2521 ggcattacag agaaagaagc gggtagttta ctggtgcgac ttatccagaa tgaaaaggtt
     2581 tgttgctttg agatcgccga agtcaatccg accctcgata aagaaaacct gatggctgaa
     2641 aacacctttg agatccttca acgggtggtc agccagctcg cctgattccc agcgtacgat
     2701 atgtcaattc aacgattcca ggataccatt cgccaatcgg tggtcaatgc cgttcaagac
     2761 cggtttggaa aatccatcgc ccctgaccag gtggtattcc aggagaccaa gcgggagttt
     2821 gaaggggata tcaccctgtt ggtattccca ctgaccaagg tttccggcct gtcacccgag
     2881 ctgaccgccg aacagttggg aggttacctc gttgaaaatt ccggtatcgt cacaggtttc
     2941 aatgtcgtca aaggcttttt aaacctggtc attgccgatc gtttctggaa cgatttcttt
     3001 caggccattg ccgctgatac ccggcaattg tttgatgcag ggaagacggc gcgtcgccgt
     3061 ttgatggtag agtattcttc gccgaatacc aataaacccc tccaccttgg gcacatccgg
     3121 aacaacctgc tcggctactc cgtcgcggag atcctcaaag ccgccggcca tgatgtcacg
     3181 aaggtgaacc tcgtcaacga ccggggcgtt cacatctgca aatccatgct ggcctggaaa
     3241 aagttcggtc atggcgagac gcccgaatcg agtgggatga aaggcgacca cctggttggc
     3301 aagtactacg tcgtgtacga caagaaactc aaggagcaga tcgaggaagg caagaaagcc
     3361 ggtcttccgg aagaagcggc cgagaaacag gcgccactga cccttgaagt ccaggagatg
     3421 ttgcgccaat gggaagccgg cgatccggag accattgctt tgtggaaacg gatgaacggc
     3481 tgggtctacg aaggctttga tgagacttac cggctcatgg gggtggactt cgacaaaacc
     3541 tattacgaat ccgaaaccta cctcttggga aaacgaattg tcgatgaagg tctgtcccaa
     3601 ggggtgttct atcgcaaaga cgatggctcc gtctggattg atctgacgga tgtcggactt
     3661 gatcagaaat tgctcctgcg ctccgacgga acttctgtct atatcaccca ggacctgggt
     3721 accgcgcagt tgcgctacga tgagtttaag ggcttggaat cattgattta tgtagtcgga
     3781 aatgaacaag aatatcattt caaagtactg cgggaaatct tccggaaact gggccgggaa
     3841 tgggctaccg gtttatacca tctgtcatac gggatggtcg acctgccgtc cggcaagatg
     3901 aaatcccgag aagggaccgt cgtggatgct gacgacctga tccgtgaaat tgtcgaacag
     3961 tgcgaaatgg cgaccaaggc gcaggggaag atcgacgatt tcgattcagc ggaagcccgg
     4021 catttgtacc acacgcttgg aatgggcgcg ttgaaatact tcatcctcaa agtggatccg
     4081 aagaagcgta tgctcttcaa tccggcggag agcattgata ttaacggcaa caccggccca
     4141 ttcattcagt atgcccatgc ccgtatatgt tccgtattga ggaaaggatc ggcggccaat
     4201 ctggaaaagg ggcttgccgg aacgcaccgg ccggatgaag gtgagaagga actgctacgg
     4261 gtcctgtatc gatatccgga agtcctgcaa gatgccgcca acggctactc cccggcgctg
     4321 atcgcgaact atgcctacga gctggcgaaa agtttcaatc acttttggca cgaccaccag
     4381 atcatcgatg ccgccaaccc gggtgcctct gcattccgtc tcgagctaag cagggtaacg
     4441 ggatcggtta tcaaggaagc cttaaggttg ctcggtatcg aagcg
//
LOCUS       DAOTON010000115         4459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15932_length_4459_cov_4.504087, whole genome shotgun sequence.
ACCESSION   DAOTON010000115 DAOTON010000000
VERSION     DAOTON010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15932_length_4459_cov_4.504087"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="P5292_03350"
     CDS             complement(<1..271)
                     /locus_tag="P5292_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRS38193.1"
                     /translation="MQKRPLADATRLDITMQRLCYQLIENHGDFSKTALIGLQPRGVF
                     VARRLKAHLERILEGRKIRLGELDVTFYRDDFRRREILAPSATNMD"
     gene            complement(320..790)
                     /locus_tag="P5292_03355"
     CDS             complement(320..790)
                     /locus_tag="P5292_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRS38194.1"
                     /translation="MQTSLQFLLEEFPRQLRMIDPATPPVFGKMNVHQMIEHLCEAVR
                     NANGKDVFPEILTPAERVPAMQEFVVGPKEFRPNTVNQLMPVEPLPAKMTSMEELIRQ
                     LEAELHDFVKCFEGQADKRIRNPFFGDLNFEQWVALLAKHARHHLRQFGWQQPA"
     gene            complement(909..2747)
                     /gene="rpsA"
                     /locus_tag="P5292_03360"
     CDS             complement(909..2747)
                     /gene="rpsA"
                     /locus_tag="P5292_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRS38195.1"
                     /translation="MITEEITNQSAAPKADPFNWETSGKGWNNYSAEERTRMEKMYDQ
                     TLNAVVEHDIVEGIVVSVSPKDVLINIGYKSDGLIPLTEFRHMPDIKAGDKVEVYVEK
                     QEDKNGQLLLSHKKARALKSWDRINAALEGDEVITGYVKCRTKGGLIVDVFGIEAFLP
                     GSQIDVKPIRDYDQFVGKNMEFKVVKINNEFKNVVVSHKILIEEELELQKAEIMKKLE
                     KGQVLEGTVKNITSYGVFMDLGGVDGLLHITDISWGRISHPEEVLKLDQKINVVILDF
                     DEEKKRIALGLKQLTPHPWTQLDGNLNVGDKVKGKVVVLADYGAFVEIAPGVEGLLHV
                     SEMSWSQHLRSPHDFLKVGEEIECVVLTLDREERKMSLGLKQLKPDPWVNVITKYPAG
                     TKLTATVKNFTNFGVFVELEEGIDGLVHISDLSWSKKIKHPSEFTKVGDKLDVVVLEV
                     DAENRRLSLGHKQLEENPWDVFETIFGVDSLHEGTVLKVGEKGATVALPYGVEGFCPN
                     KHLVKEDGKSLKADDKADFKVIEFNKDQRKIVVSHARIHEEARGIARVAEKETKEKDR
                     EDNAKAVKKVKENVEKTTLGDIAALSNLRTELEAAEKKAQDSDKKD"
     gene            complement(3068..3958)
                     /locus_tag="P5292_03365"
     CDS             complement(3068..3958)
                     /locus_tag="P5292_03365"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRS38196.1"
                     /translation="MKITLDPHSGFCFGVVYAIQMAEDELDRSGSLYCLGDIVHNNVE
                     VERLKAKGLKIIDHETLRQLHDCKVLIRAHGEPPETYQIALQNNIELIDASCPVVLKL
                     QNRVRSSYEEVVANNGQVVIYGEIGHAEVNGLVGQTGGEALIIRNEDDLSKIDFSRPI
                     YFFSQTTKSSRGFERMKALIEERSRLAQESEIQDEYLSTNDTICRQVSNREPQLRKFA
                     EQHDLILFVSGKKSSNGKALFEVCRAVNPRTYFISDVEEIDYAWFDGVQSIGICGATS
                     TPMWLMQKVASHLEPIGTSA"
     gene            complement(4006..>4459)
                     /gene="cmk"
                     /locus_tag="P5292_03370"
     CDS             complement(4006..>4459)
                     /gene="cmk"
                     /locus_tag="P5292_03370"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRS38197.1"
                     /translation="VHLNGLNVERQIRDLRISDLVSPVSAIPAIRRRMVKKQQGYGRD
                     KGIVMDGRDIGTTVFPNAELKIFMTARPEVRAKRRFDELNEKGFMVTMDEVSKNIEDR
                     DHTDTHRAESPLRKAEDAIVLDNSDLNQEEQLAIALDLVRQRSVQSPA"
BASE COUNT          933 a         1176 c         1189 g         1161 t
ORIGIN      
        1 aatccatatt cgttgcactg ggggcaagga tctctctgcg gcggaaatcg tcccgataga
       61 aggtcacgtc cagttctccc aggcggatct ttcggccttc caggatgcgt tccagatgcg
      121 ctttcaagcg gcgggcgacg aaaacgcctc gcggctggag tccgatcaat gcagtcttgg
      181 aaaaatcccc gtgattctcg atgagttgat agcacaatcg ctgcatcgtg atgtcgaggc
      241 gggtggcgtc ggccaggggg cgtttctgca tagtcgatcc caaggtagga tgtttaaggg
      301 gctgttccaa tgtccgcgat caggcaggtt gttgccagcc gaattgccgc agatgatggc
      361 gggcgtgctt ggccagtaac gctacccact gttcgaagtt gaggtcgccg aagaatgggt
      421 tacggatccg tttatcggct tgcccttcaa agcacttgac aaaatcgtgc agctctgctt
      481 ccaactgccg gatcaactcc tccatactgg tcatcttagc aggaagcggt tccaccggca
      541 tgagttgatt gacggtattg ggacgaaact ccttgggacc aacgacgaac tcctgcatgg
      601 ccggcacgcg ttcggccggc gtaaggattt caggaaatac gtctttgcca ttggcgttac
      661 gcaccgcttc gcaaagatgt tcaatcatct gatgaacgtt catctttccg aacacaggtg
      721 gcgtagcggg gtcgatcatt cgtaactggc ggggaaactc ttccaggagg aattgcaggg
      781 atgtttgcat ggtcggttgg ctggtcaact gctaaggtgc ataggaatat tcagcccgga
      841 aaaagaaaaa agccgtccca ggggacggct ttttctttct tgagcttctg aattcaacat
      901 tatgaagttc agtccttttt atcagagtcc tgagctttct tttcagcggc ttcgagttcg
      961 gtacgcaggt tgctgagggc cgcgatatcg ccaagggtcg tcttttcaac gttctccttc
     1021 actttcttca ctgcctttgc attatcctcg cgatccttct ccttggtctc cttctcggca
     1081 acacgcgcga ttccgcgggc ttcctcgtgg atacgggcat gggatacgac gatcttacgc
     1141 tggtctttgt taaactcgat gaccttgaag tcggctttgt catcagcttt gagggacttg
     1201 ccgtcttcct tcaccaggtg cttgttcggg cagaagcctt cgacaccgta gggaagcgcg
     1261 actgttgcgc ccttctcacc gactttcaat accgtgcctt cgtgcaggct gtcgacgccg
     1321 aagatggttt cgaacacatc ccaggggttc tcctcgagtt gtttgtgtcc gaggctgagg
     1381 cgacggtttt ccgcatccac ttcgagcacg accacgtcga gtttatcacc cactttggtg
     1441 aactccgacg ggtgcttgat cttcttgctc caggagaggt cggagatgtg cacaagtccg
     1501 tcgatgcctt cttcgagttc cacgaagacg ccgaagttgg tgaagttctt caccgtagcg
     1561 gtcaacttcg tgccggccgg gtatttcgtg atgacgttca cccacggatc aggcttgagt
     1621 tgcttgaggc cgagtgacat cttacgctct tcacggtcaa gggtcaggac aacacactcg
     1681 atctcttcgc cgactttcag gaagtcgtgc gggctgcgga ggtgctgcga ccaggacatt
     1741 tccgatacgt ggaggagtcc ttcgacaccc ggagcgatct cgacgaacgc gccgtagtcg
     1801 gcgaggacaa ccactttgcc cttcacctta tcgccaacgt tgaggttgcc atcgagctgc
     1861 gtccaaggat gcggagtaag ctgcttgagg ccaagcgcga tgcgcttctt ctcttcgtcg
     1921 aaatcgagga taacgacgtt gatcttctga tcgagcttga gtacttcttc cggatgcgag
     1981 atgcggcccc aggagatatc cgtaatgtgc agcaggccgt cgacaccacc gaggtccatg
     2041 aacacaccgt aggaggtgat gttcttgacg gttccctcga gtacttgacc cttctcgagc
     2101 ttcttcatga tctcggcctt ttggagctcg agttcctctt cgatcaggat cttgtgagag
     2161 acgactacgt tcttgaattc gttgttgatc ttcaccacct tgaactccat gttcttgcct
     2221 acgaattggt cgtagtcgcg gatcggcttg acgtcgatct gggagccggg caggaaggcc
     2281 tcgatgccga agacgtcgac gatcagacca cccttggtac ggcacttcac atagccggtg
     2341 atgacctcgt caccttccag ggccgcgttg atgcgatccc aggacttcag tgcacgggct
     2401 ttcttgtgag aaagcaggag ctgaccattc ttgtcctctt gcttctccac gtaaacttcc
     2461 accttgtcgc cggctttgat gtccggcatg tggcggaatt cagtcagcgg aatcagacca
     2521 tccgatttgt agccgatgtt gatgagcaca tccttgggcg aaacggatac gacaattcct
     2581 tccacgatgt cgtgttcgac caccgcgtta agggtttgat cgtacatttt ctccatccgg
     2641 gtacgttcct cggcggagta attgttccag cctttgcccg aagtttccca gttgaaggga
     2701 tctgctttgg gagcagcgga ttggttcgtg atttcttctg taatcattta atttttaagt
     2761 tgtttatagt taagtggcgt cgtttctgtt tggggtcgca aatttaggaa ttatcttctt
     2821 tggttaaaag tgctcaatta gaaactttta aaggccatta gcagtctgcc atgacttgaa
     2881 attttgattt tttcaagggg taggaagcac cactacccga ttgaaggatc aggataattg
     2941 attacgacca tatatttaac ataataatac acaaatggga caaaatcctg tatttactcc
     3001 tctatggact aaaaggttgc aaaacattga aatgaaagtg gggaaataac caatccggaa
     3061 ccccggatca ggcagaagta ccgatcggtt cgaggtggct ggcgaccttt tgcatcagcc
     3121 acatcggcgt actggtcgcg ccacaaatgc cgattgactg cacaccatcg aaccaggcat
     3181 agtcgatctc ctccacgtcc gaaatgaaat aggtcctggg gttgaccgcg cgacagacct
     3241 cgaacagcgc ttttccattg gaactcttct tcccggaaac aaacaggatg aggtcgtgct
     3301 gttccgcgaa ctttcgaagc tggggttccc ggttggaaac ctgccggcag atggtatcat
     3361 tggtggaaag gtactcgtcc tggatctcgc tttcctgtgc aagtctcgac cgttcctcaa
     3421 tcaacgcctt catgcgttca aagccgcggg aactcttcgt agtctgagag aagaaataga
     3481 tcgggcggga gaaatcgatc ttactgaggt catcctcgtt gcggatgatg agggcctccc
     3541 ctcctgtttg cccaaccagg ccgttcactt cggcatgtcc gatctctccg tagatgacga
     3601 cctgtccgtt gttcgccacc acttcttcgt aactggagcg gacgcggttc tggagtttga
     3661 gtacaacggg acaagaggca tcaatcaact cgatgttgtt ttgcagcgcg atctgatagg
     3721 tctccggtgg ttcaccgtgc gcacggatta gcactttgca atcgtgcagt tgccgcaagg
     3781 tctcatggtc tatgatcttc agccctttgg ctttcagccg ctcgacctcg acgttattgt
     3841 ggacgatatc accgaggcaa tagagtgaac cggaacggtc cagttcatct tcagccatct
     3901 ggatggcgta aacaacgcca aaacaaaagc ccgaatgcgg gtctaaggtg attttcattc
     3961 gagatgcaaa ggtacgaaaa agcgctcgaa ccagttaact ggttctcaag ccggtgattg
     4021 cacggagcgc tgccggacca ggtccagcgc gatagccagt tgctcttcct ggttgaggtc
     4081 ggaattgtcg agcacgatcg cgtcctccgc cttcctaagc gggctttcgg cccgatgggt
     4141 atcggtatgg tcacggtcct cgatgttctt gctgacttcg tccatcgtca ccatgaagcc
     4201 cttttcgttc agttcgtcga accgccgttt ggcacgcact tccggtcggg ccgtcatgaa
     4261 aatcttcagc tccgcattcg ggaataccgt agtgccgata tcccgcccat ccatgacgat
     4321 ccctttatcg cgtccatagc cttgttgttt ctttaccatc ctccggcgaa tggctggaat
     4381 ggcgctgacc ggacttacca gatccgagat acgcaggtca cgaatctgtc gttccacgtt
     4441 cagcccgttc aggtgaact
//
LOCUS       DAOTON010000116         4456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_15946_length_4456_cov_4.179505, whole genome shotgun sequence.
ACCESSION   DAOTON010000116 DAOTON010000000
VERSION     DAOTON010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15946_length_4456_cov_4.179505"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="P5292_03375"
     CDS             <1..856
                     /locus_tag="P5292_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS38198.1"
                     /translation="MDIEPEVPAVLKGDPVRLNQILINLAGNAIKFTEKGSVRIHVKQ
                     LSRQADESILEFSVSDSGIGISEEQINKIFESFSQAGHDTARKFGGTGLGLTISKQLI
                     ELQGGSIYVRSELGKGTTFSFKIPYRIGDAARLVATEATGTDEAVQALKGVRILLVED
                     NQFNQMVAVDTLQDMLLEPTIDLAENGQEAVEKVRENDYDLVLMDVQMPVMNGFEATR
                     QIRNLSGPRSRTKIMAMTANVTEEEINNCFACGMDEYLAKPFDQTKLLQKLVRLYLKR
                     AETTNQAT"
     gene            853..1194
                     /locus_tag="P5292_03380"
     CDS             853..1194
                     /locus_tag="P5292_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRS38199.1"
                     /translation="MTNLTDMTFLDGFTGGNADKKKKYISMFLQLCPSALDSMKASLD
                     QQDYDALRATAHSLKPQITYMGIKAGEPMVKDIEHRAGQRVEVEKLPALFSEFDQLCR
                     QAMEELRQRIA"
     gene            1256..1774
                     /locus_tag="P5292_03385"
     CDS             1256..1774
                     /locus_tag="P5292_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS38200.1"
                     /translation="MNTVKNIFVVDDDQTQAMMLQDYLSKYSTFTIHTFNSGEECIKN
                     LSLKPDIIFLDYNFDKVGKDAMNGIEILREIKSANPSTEVVMFSGQDRIEVAVNSMKY
                     GAFDYIVKNESAFHRSENVIFNIIKRLKLQYQASLYKKLTIFLGITLLLVIILVVVLY
                     QSGVISKNPGWM"
     gene            1879..3033
                     /locus_tag="P5292_03390"
     CDS             1879..3033
                     /locus_tag="P5292_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HRS38201.1"
                     /translation="MPTFPGSLQSKLPNVGTTIFTVMSALAQEHQAINLSQGFPNYPV
                     SPEIIESIHRYLRDGFNQYAPMPGVQILRERIAEKMESLYGARYHPDSEITITSGGTQ
                     AIYTAITAVVRPGDEVIVIEPAYDCYVPAIELAGGLPVYVQLELPSGKVDWEQVRKAI
                     RPRTRMIIVNTPHNPTGSCFDASDLETLKQLTDGTEILILSDEVYEHILFDGRIHQSV
                     ARHPELADRSMVVFSFGKTYHATGWKMGYCLAPANLMKEFRRVHQFVVFCANTPFQYA
                     LADAMQTRDYLGIPEFLQAKRDRFLELLKGSRFRFSPAGGSYFQLLDYSAITDEPDTD
                     FAIRLTKEAGVASIPVSVFSHHYGDQRLLRFCFAKNDETLEQAAERLRNV"
     gene            3042..3821
                     /locus_tag="P5292_03395"
     CDS             3042..3821
                     /locus_tag="P5292_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRS38202.1"
                     /translation="MSLRIALLQADLRWEDPTANRELLSGMIDKIRERPDLILLPEMF
                     ATGFSMLADTLAQDMDGPDVRWMQSVAERSGATVTGSLIIEENGRYYNRLLWMSPNGL
                     SGMYDKRHLFRLAHEQDTYTAGTTRPVFRLNDFRIAPLICYDLRFPVWSRRSPAYDYD
                     LLLYAANWPEKRGHAWRSLLPARAIENQSYVAAVNRVGADGHGMDHRGDSVVLDYRGE
                     VISDSEPGSPSVLEATLELDPLVRFREQFAFWQDADRFRIE"
     gene            3854..>4456
                     /locus_tag="P5292_03400"
     CDS             3854..>4456
                     /locus_tag="P5292_03400"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRS38203.1"
                     /translation="MRTIVLLFTLIVLHTASAGQDHRISRAERDAFPAYRDSVRRAFR
                     LNGIDQPPSYSVRAAAEWEEIDALVIAWTGFPDILREIVRGAQTECRVLIVCTDSTIV
                     KNNLQSNGVPLTNIEYVVAPFNTIWCRDYGPWNVYDERNDSLYLIDWIYNRPRPKDDT
                     VNTSLERYTSLPMYRTITTPWDLVHTGGNFMVDGMGTAFSS"
BASE COUNT         1052 a         1241 c         1179 g          984 t
ORIGIN      
        1 catggatatc gagcccgaag taccggccgt cctgaaggga gatccggtcc gcctcaatca
       61 gattctgatc aacctggccg gcaacgcgat caagttcacg gaaaaaggct ccgtccgcat
      121 acacgtcaaa caactcagcc ggcaggccga tgagtcgatc ctggaatttt cggtttccga
      181 ttccggcatc ggcatttcgg aagaacagat caacaagatt tttgaaagct tctcacaagc
      241 cggtcacgat accgcccgca aattcggtgg caccggcctg gggcttacca tttccaagca
      301 actcatcgag ctacagggcg gcagcattta cgtcaggagt gaattgggga aagggaccac
      361 cttcagcttc aagattccct atcggatcgg tgatgctgcc cggctggtgg caacagaagc
      421 caccggcacc gatgaggcgg tacaagccct gaaaggagtt cgcatcctgc tggtggagga
      481 caaccaattc aaccagatgg tggcggtcga taccctgcag gacatgctcc tggaacccac
      541 catcgacctg gccgaaaacg gacaggaagc ggtcgaaaag gtgcgcgaaa acgactatga
      601 tctggtgctg atggacgttc aaatgcccgt aatgaacggt tttgaagcaa cccggcagat
      661 tcgtaaccta tcgggccccc gtagtcgtac gaagatcatg gccatgacgg ccaatgtaac
      721 cgaggaggag atcaacaatt gttttgcatg cggtatggat gagtaccttg caaagccctt
      781 tgaccagacc aagctgctcc aaaagctggt acgattatat ctgaagcgag ctgaaacaac
      841 caatcaagcg acatgaccaa tctgactgac atgaccttcc tggacggatt cacgggaggt
      901 aatgccgaca aaaagaaaaa gtatatcagc atgttcctcc agttatgccc ttccgcgctg
      961 gatagcatga aagccagtct cgaccagcag gattacgatg cgttgcgtgc aaccgcgcac
     1021 agccttaaac cccagatcac ctacatgggc atcaaggcgg gagaacctat ggtgaaagac
     1081 attgagcacc gggccggaca gcgtgtggag gtggagaaac ttcccgcttt gttctccgaa
     1141 ttcgaccaat tatgccgtca ggcgatggaa gaattgcgtc aacggatcgc ctgaggggta
     1201 aacaccctcc atttttccaa aataaactac atttgccccc ttcaagctcc cgctcatgaa
     1261 tacggtaaag aacatttttg ttgtagatga cgaccagacg caggcgatga tgctgcagga
     1321 ttacctgagc aaatacagca ccttcaccat ccataccttt aattcgggtg aggaatgcat
     1381 caagaacctg tcattaaagc ccgatatcat cttcctcgac tacaacttcg ataaagtcgg
     1441 gaaggatgcc atgaacggta tcgagatcct gcgcgagatc aagagtgcca atccgagtac
     1501 ggaggttgtg atgttcagcg gtcaggaccg catcgaagta gccgtcaatt ccatgaagta
     1561 tggcgcgttc gattacatcg tcaagaatga aagcgcgttc caccgttccg agaacgtcat
     1621 cttcaacatc atcaagcgtc taaagctcca gtaccaggcc agcctttata agaaactgac
     1681 gatcttcctt ggcatcacct tgcttctggt gatcatcctg gttgtcgtgc tttatcagtc
     1741 cggcgtcatt tcgaagaacc cgggatggat gtaaaataat ccgaatcagg tatccgaagc
     1801 cggccggttg aaactggccg gcttcgtcgt tttcaggcgt ggatggtctt ctgtatcttt
     1861 tcgccacaat cggacgacat gcccaccttt ccaggctccc tacagtcaaa actcccgaat
     1921 gtcgggacga ccatcttcac ggtgatgtcg gcgttggcgc aggagcatca ggccatcaac
     1981 ctctcgcaag ggtttccgaa ctatccggtt tccccggaga tcattgaaag catccaccgg
     2041 tatctgcggg atgggttcaa ccaatatgcg ccaatgccgg gcgtgcagat cctgcgtgaa
     2101 cgcatcgcgg aaaagatgga atccctgtat ggcgcacgct atcatcccga ttccgaaatc
     2161 accatcacca gcggtggcac acaggcgatc tacacggcta tcacggcggt cgttcgcccc
     2221 ggtgacgaag tgatcgtgat cgaacccgct tatgattgct acgtaccggc gatcgaactc
     2281 gccggtggtc tgccggtgta tgtgcaactg gagctgccat cgggaaaagt cgattgggag
     2341 caggttcgaa aggccatccg tccgcgcacg cgaatgatca tcgtcaacac tccgcacaac
     2401 cctaccggtt cctgtttcga tgcatccgat ctcgaaacgc tgaaacagtt gacggacgga
     2461 accgagatcc tgatcctgag tgacgaggtc tatgaacaca tcctgttcga cggccgtatt
     2521 catcagagtg ttgcccgaca tccggaactg gccgaccgtt cgatggtggt cttctccttt
     2581 gggaagacct atcatgctac cggctggaag atgggctatt gcctggcgcc tgccaatctc
     2641 atgaaagaat ttcgtcgtgt acaccaattc gtggtattct gcgcgaatac gcctttccaa
     2701 tacgcactgg ccgacgcgat gcaaacccgc gattaccttg gaattccgga gttcctccaa
     2761 gccaaacggg atcgcttcct cgaattgctg aaaggatcgc gtttccgttt ttcgccggcc
     2821 ggcggctcgt attttcagtt gctcgattac agtgcgatca ctgacgagcc cgataccgac
     2881 ttcgcgatcc gtcttacaaa agaagccggc gtcgcttcca ttcctgtttc cgtattcagt
     2941 catcattacg gtgaccagcg cttattgcgg ttctgcttcg cgaaaaacga cgagaccctg
     3001 gaacaagccg ctgaacgcct tcgcaacgtt tgagcgatcc catgtcactt cgcatcgccc
     3061 tgcttcaagc cgaccttcgc tgggaggatc ctacagccaa ccgggaattg ttatccggga
     3121 tgatcgacaa gatccgggag cgcccggatc tgatcctgct acccgagatg ttcgctacgg
     3181 gtttcagcat gctggccgac acccttgcgc aagacatgga tggaccggat gtccgttgga
     3241 tgcaaagcgt cgcagagcgc agcggggcta cggttaccgg gagtctcatc atcgaagaga
     3301 acggacgata ttataatcgc ttgctgtgga tgagtccgaa cggcttgtcg ggcatgtatg
     3361 ataagcggca cttgttccgg ttggcgcatg aacaagacac ctatacggcc ggtactaccc
     3421 gccctgtttt ccgcctcaac gactttcgca tcgcgccgct gatctgctac gatctgcggt
     3481 tcccggtctg gagtcgaaga agtccggctt atgattatga cttgttgctt tatgcagcca
     3541 actggccgga gaagcgcgga catgcatggc gctccctctt gcccgcccgg gccatcgaga
     3601 atcagtcgta cgttgcggca gttaaccggg tcggagcaga cggccacggc atggatcatc
     3661 gcggtgattc ggtagtactc gattaccgcg gagaagtgat atccgactcc gaaccgggaa
     3721 gtccttcggt cttggaggcc acattggaac ttgatccgct tgtacgattc cgggagcaat
     3781 tcgccttctg gcaggatgcc gaccggttcc ggatcgaata agcttacctt gcaacatccg
     3841 gctgtcacgg cgaatgagaa cgatcgtact cctgttcacg ttaatagtgt tgcacaccgc
     3901 gtctgctggt caggaccacc gtatcagcag ggccgaacgc gacgcattcc ccgcgtatcg
     3961 tgattccgtc cgtcgggcat ttcgtctcaa cgggattgat caaccacctt cctattcggt
     4021 tcgtgctgcg gccgaatggg aagagatcga cgcgttggtg atcgcctgga cgggattccc
     4081 cgacatcctg cgggaaatcg tacgcggagc gcagaccgaa tgccgtgtgc tgatcgtttg
     4141 taccgattca acgatcgtga agaacaacct gcagtcgaat ggcgtaccgc ttacgaatat
     4201 cgagtacgtc gtagcgccgt tcaataccat ttggtgccgc gactatggtc cctggaatgt
     4261 gtacgacgag cgcaacgact cgctctacct gatcgactgg atctacaatc gacccaggcc
     4321 gaaagacgat acggtcaaca cctcgctgga gcgctacaca tccttaccca tgtaccgcac
     4381 catcactaca ccctgggacc tggtgcatac cggtggcaat ttcatggtcg atggaatggg
     4441 aacagccttt tcgtcg
//
LOCUS       DAOTON010000117         4439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16036_length_4439_cov_4.127281, whole genome shotgun sequence.
ACCESSION   DAOTON010000117 DAOTON010000000
VERSION     DAOTON010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16036_length_4439_cov_4.127281"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2629
                     /locus_tag="P5292_03405"
     CDS             <1..2629
                     /locus_tag="P5292_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38204.1"
                     /translation="GQWAFLGLFGVFMMASCSQTKDTFINRTFHNLSGHYNGYYNAGV
                     KLLEGTDRLAEQHEDHYDRTLRVYRYANAEKAKSVYPQMDDVIKRTGTVISRHTIYDK
                     NGNEKPNSEHWIDDNWLLYGKALFFKHEYFAAMEAFNYVEVTYKKETTRHLASLWIAK
                     TYLELTQLNEAENKLDYVRNQSDFPKKNRWELEATFADYYVQVRNNSKAIEHLTRAAE
                     LAPRRDDRIRFLFILGQLQQAEEKFSLAFDTYSRVIKMNPPYEMGFNARINRARCYEA
                     GGKGSDEVRNELAKLQKDPKNKDFLDQIYYALAGLEKKSGNEPGEIEYLNKSVQASTT
                     NKNQKALSYLELAKIAFVKPDYRLAKGYYDSTIANLSNDHPDYTEVLQRRNSLTRLVR
                     YLNIIQNEDSLQSLAHMTPEQRAKMVEDQIRKEEEEKARQKEEEQSNQIFQPGNNPQQ
                     ANQFNKEGGSNWYFYNQQATAFGFNEFTRRWGNRKLEDNWRRSSREQVFQATEEKEDT
                     LVTEAKEIADPKAAAEAKKKAMLDAIPATPEAIEKSTNKIIDAYYNAAMIYREQLKDN
                     SSAAEMFEELLRRFPENKFKLQSMYQLYRLYAQLGNPTKSEYYKNIILNQYGDTEYAE
                     IIRNPNYGQDMATRKSQLELFYEETYRKYLNGEYTAVIQRRSQAETMFPQNAFMPKFD
                     LLKAMSIGKTQTRNNFEASLQDVVRTWADDPVREAAQDMLDYLQQQGGGAAPAADTAR
                     STPVEQPAATARNYNYFPDTTHYVIVVFQAIGGALDANRFKTKLSNFNTANFGSKGLQ
                     TQDLMLDHRMKLVVIKNFANKAEAMAYFSALYDNDEVYGVVDVSGYQQYAVSINNFPE
                     IIRQKKLDEYEDFFRSFYR"
     gene            2875..3306
                     /locus_tag="P5292_03410"
     CDS             2875..3306
                     /locus_tag="P5292_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRS38205.1"
                     /translation="MFKQNKAAGSPGKSNDPNTASINLIGAGTVIDGDIRSNGDIRID
                     GTVNGQVISKAKVVVGSTGLVEGDVMCQNADVSGTIKGKTTVSELLFLKSTAKVIGDI
                     VTGKLVVEVGATFTGSCNMGPVIKDIKDGDQPTLSKEKSAI"
     gene            3320..3496
                     /locus_tag="P5292_03415"
     CDS             3320..3496
                     /locus_tag="P5292_03415"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRS38206.1"
                     /translation="MRYATMGTQMLVIMGAMTFGGYWLDKHFGLQFPIFTVVLSLFGI
                     AAAFYLSLKDLLKK"
     gene            3672..4043
                     /locus_tag="P5292_03420"
     CDS             3672..4043
                     /locus_tag="P5292_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38207.1"
                     /translation="MFRYLVTLVIFTLVLAGVLYGVQSSRPEWVGESSWWMLGYFFLL
                     TAAFHWGLVAASKGNPQAFIRYYMGATSFKLLIHLGVLVLYAFFYGEHVVRFIVTFLF
                     GYVLFTGLEVFLSWNRFRRAG"
     gene            4157..>4439
                     /locus_tag="P5292_03425"
     CDS             4157..>4439
                     /locus_tag="P5292_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRS38208.1"
                     /translation="MLYPEITDEIIRSFYNVYKILGYGFLEKVYENSMIIDLLRRKRY
                     VEKQVPIDVHFDGHRVGKYFADLLVDRRVIVEIKAVEYLIPEHELQLINY"
BASE COUNT         1150 a         1148 c         1177 g          964 t
ORIGIN      
        1 cggccaatgg gcctttttag gccttttcgg ggtctttatg atggcgtcct gctcccagac
       61 caaggacacg ttcatcaacc gcacctttca taatctgtcg ggtcactaca acggctacta
      121 taatgccggg gtgaagctgc tggagggaac cgaccgattg gcggagcagc acgaagatca
      181 ttacgatcgt accctgcggg tttatcgcta tgccaatgcg gaaaaggcga agtcggtcta
      241 tccgcagatg gacgatgtga tcaagcgtac cggcacggtc atttcgcgtc acaccatcta
      301 cgacaagaac ggcaacgaga aaccgaactc cgaacactgg atcgacgaca actggctgct
      361 ttacggcaag gcgctgttct tcaagcacga gtacttcgcc gcgatggagg cgttcaacta
      421 cgtcgaggtt acttacaaaa aggaaacgac ccgtcacctg gcttcgctgt ggatcgccaa
      481 gacgtacctg gaacttacgc agctcaacga agcggaaaac aaactcgact acgtccgcaa
      541 ccaaagtgat tttccgaaaa agaatcgttg ggaactggaa gccacctttg ccgactacta
      601 cgtgcaggtg cgcaacaatt ccaaagccat cgaacacctt acccgtgcgg ccgaattggc
      661 tccccgccgc gacgaccgca tccgcttcct gttcatcctt ggacaattgc aacaggcaga
      721 ggagaaattc tccctggcgt tcgacacgta ttcgcgcgtt atcaagatga acccgccgta
      781 cgaaatgggc ttcaacgccc gtatcaaccg tgcccgttgt tacgaggccg gcggcaaagg
      841 cagcgacgaa gtgcgcaacg aactcgcgaa gttgcagaag gatccgaaga acaaggactt
      901 cctcgaccag atctattacg cgctggccgg cctggagaag aagtcgggca acgagccggg
      961 agagatcgaa tacctgaaca aatcggttca ggccagcacg accaacaaga atcaaaaagc
     1021 gctttcctac ctcgagctcg cgaagatcgc gttcgtcaag cccgactatc gactggccaa
     1081 aggatattac gacagtacca tcgccaacct gtccaacgac catccggact acacggaagt
     1141 cctgcagcgc cggaacagtc ttacccgttt ggtgcgttac ctgaacatca tccagaacga
     1201 agacagtttg cagtcgctcg cccacatgac accggaacag cgcgccaaga tggtggaaga
     1261 ccagatccgg aaagaagagg aagagaaggc acggcagaag gaagaagagc agtccaacca
     1321 gatcttccag ccgggcaaca acccgcaaca agctaatcag ttcaacaagg aaggcggttc
     1381 gaactggtat ttctacaatc agcaggcgac cgcgttcggc tttaacgagt tcacccgccg
     1441 ctggggaaac cgtaaactcg aagacaactg gaggcgcagt tcccgtgagc aggtattcca
     1501 ggctacggaa gaaaaagaag acaccctggt tacggaagcc aaggagatcg cggatccgaa
     1561 agccgctgcc gaagccaaga agaaggcgat gctcgacgcg atccccgcca cgccggaggc
     1621 gatcgagaag tcgaccaaca agatcatcga cgcctattac aacgcggcca tgatctaccg
     1681 tgaacaactg aaggacaatt cgtctgcggc ggagatgttc gaggagttgt tgcgccgctt
     1741 cccggagaac aagttcaagc tgcagtcgat gtaccagttg taccggctgt acgcgcaact
     1801 gggcaacccg acgaagtcgg agtattacaa gaacatcatc ctcaaccagt atggagatac
     1861 cgagtacgcg gagatcatcc ggaatccgaa ctacggacag gacatggcca cccgtaagag
     1921 ccagttggaa ttgttctacg aggagacgta ccgcaaatac ctgaacggcg aatacacggc
     1981 cgtgatacaa cgtcgttcac aggccgagac catgtttccg caaaacgcgt tcatgccgaa
     2041 gttcgacctg ctcaaggcga tgagcatcgg caagacgcag acgcgcaata acttcgaagc
     2101 gtctttgcag gatgttgtgc gtacctgggc ggatgatccg gtgcgggaag cggcgcagga
     2161 tatgctcgac taccttcaac agcagggtgg aggcgcggca ccagccgcag ataccgcgcg
     2221 ttcaaccccc gtagagcaac cggctgccac ggcccgcaac tacaattatt tccctgacac
     2281 gacgcactac gtgatcgtgg tcttccaggc gatcggcgga gcactcgatg cgaaccggtt
     2341 caagaccaag ctgtcgaact tcaacaccgc gaatttcggc tcaaaggggt tgcagacgca
     2401 ggacctcatg ctcgaccatc gcatgaaact tgtcgtgatc aagaatttcg cgaacaaagc
     2461 cgaagcgatg gcctacttca gcgcactgta cgacaacgac gaggtgtacg gtgtagtcga
     2521 tgtcagcggt taccagcaat acgcggtatc catcaacaac ttccccgaga tcatccggca
     2581 gaaaaagctg gatgagtacg aggacttctt ccggtcgttt taccggtgag gagtagaaag
     2641 tttcgagttt ccggtttcgg gtttccggtt ggtttaatgg ttgatgattg aaagttgaaa
     2701 gctgatagaa tcacgtttca gatacccatt caacgcgaaa cgcgaaacgc gaaacccgaa
     2761 attcgaaacc gcgaacttat tcacccttgc gccgcattgc cggtcgtcct atatttgctg
     2821 cggttttcgt gaaaatcgcg gaaaatgagc aacttataag agaaaggagt ccccatgttc
     2881 aagcagaaca aagcggccgg cagccccggc aaatcgaacg atccgaatac cgcgtccatc
     2941 aacctgatcg gggcgggaac ggtcatcgat ggggacattc gttccaatgg agacatccgg
     3001 atcgacggta ccgtcaacgg acaggtgatc tcaaaagcca aggtggtcgt gggttctacc
     3061 ggcctggtgg aaggcgatgt catgtgtcag aatgccgacg tttccgggac gatcaagggc
     3121 aagacgacgg tttcagaatt gttgttcctc aagtcgaccg ccaaggtgat tggcgatatc
     3181 gtcaccggca agttggtggt ggaggtcggc gctaccttta cgggctcctg caacatgggt
     3241 ccggtgatca aagacatcaa ggatggcgac caaccaaccc tctccaaaga aaaatccgct
     3301 atctgataat cgcgcgctga tgcgctacgc gaccatgggc acccagatgc tcgtgatcat
     3361 gggcgccatg acgttcggcg gttactggct cgacaaacac ttcgggctgc aattccccat
     3421 cttcacggtt gtactcagtc tcttcggcat cgcggcggcg ttctatttga gtttgaagga
     3481 tttgttgaag aagtgaacag tgaagaagtg aacagtgaac agtgaatagc gaatagtttt
     3541 ttagggatga taagcaatac catagaattt gaattcactg atatttattt cagttttttc
     3601 agttactaat tactaattac caattaccaa ttaccaattt cactcccacc cactccaacg
     3661 tcccgaccgc tatgttccgc tacctggtca cgcttgtcat ctttaccctg gttcttgccg
     3721 gtgttttgta tggtgtacaa tcttcgcggc cggaatgggt aggggagtcg tcgtggtgga
     3781 tgctgggata tttttttcta ctgacggcgg cgtttcattg gggtctggtc gccgcttcga
     3841 aaggcaatcc gcaggcgttc atccggtatt acatgggcgc gacgagcttc aagttgctca
     3901 ttcacctcgg cgtgctggtc ctttacgcgt tcttctacgg cgaacacgta gtgcgtttca
     3961 tcgtgacgtt tctattcggt tatgtattgt tcaccggact cgaggtgttt ctttcgtgga
     4021 acaggttccg gcgagccgga tgattctttg tacttcgaat tgatagcacg cggatcccgc
     4081 ggatgatgcg gattcacgca gattattccc gcttttattg gatgctacca aagccaattt
     4141 gaggttactt caccttatgt tgtatccgga gatcaccgac gagattatca gatcgtttta
     4201 caatgtttat aagattctgg gctatggatt ccttgagaag gtatatgaga actccatgat
     4261 tattgacttg ttgagaagaa aaagatacgt ggaaaaacaa gtgcccatcg atgttcactt
     4321 tgatgggcat agggttggga aatactttgc agacttatta gttgaccgaa gagtgatcgt
     4381 tgaaattaaa gctgttgaat accttattcc agagcatgaa ctgcaattga ttaattatt
//
LOCUS       DAOTON010000118         4402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16190_length_4402_cov_3.395905, whole genome shotgun sequence.
ACCESSION   DAOTON010000118 DAOTON010000000
VERSION     DAOTON010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16190_length_4402_cov_3.395905"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="P5292_03430"
     CDS             complement(<1..1408)
                     /locus_tag="P5292_03430"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRS38209.1"
                     /translation="MKLRLFLGLSLIALTRFASAQTFSGTGGSIITLSDTSRFNINVS
                     GLSPASVDYTFGLESVTININHSRARDIDCFLAAPDGTLIMLTTDNGGTSGSNFTNTI
                     FRNDAATSITSGSAPFTGTYRPEGTLYNVNNGQNGNGTWQLRVIDDSNNGITGSLINW
                     SINFGGNPARPFLFSGSNLPIVVINTNGQSIPDDPKIICDMGIVNNGIGQRNHLNDPF
                     NEYNGKIAIEIRGSSSQSFPKKSYGFETRMPNGVTDTNVALLGMPSEHDWILSANYTD
                     KSFCRNVLLYQLSMEMGHYAARTRYVDVVLNGEYIGIYVLMESIKRDGDRVDIDRLHP
                     WENTAPDITGGYILKVDKTTGSGGEGWTSPYPPINHSNGQTTYLQYDYPDPDSISLQQ
                     KAYIQAYVDSFEDALSSSTFMDPINGYQKYIGNGSWIDYFFCNELSKNVDGYRISSYL
                     YKDKNKTLKAGPVWDYDIA"
     gene            complement(1552..2049)
                     /locus_tag="P5292_03435"
     CDS             complement(1552..2049)
                     /locus_tag="P5292_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRS38210.1"
                     /translation="MYKVTVNGREYQVAYDARHQSVNGEEMHPDILEYRKGKFHLLYK
                     GRSYEAELIEANFEEKSLSIKVNNTVYQLNVRDKYDDLLREMGIDITAGKKVNDIKAP
                     MPGLVLNVAVAEGQAIKKGDPILVLEAMKMENVIKAPADGAIRKISVNKGDKVEKNQV
                     MVSLH"
     gene            2106..>4402
                     /locus_tag="P5292_03440"
     CDS             2106..>4402
                     /locus_tag="P5292_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRS38211.1"
                     /translation="MSVPAPFIRLSLLSVALLLVACSGSRQLPASPPAAATSKTDPRK
                     QQEAYEAKPNRIWDLVHTRLELQPQWGSREIDGKATLFLHPHFYPTDSLVLDAKMMLV
                     KQVGRIRAQGDTLLIPFRYDSSTIRLKLDTVFHRDNELVLWIDYTGRPDLRTGDGSRA
                     IRGEKGLYFINPDGKDPNKPTQLWSQGETAHNSVWFPTLDVPNQKMTQEIYLTVDTSF
                     VTLSNGLLLSQQLNGNGTRTDYWKQSLPAPPYLTMIGVGRWSVTRERWNNIELSYYTD
                     PAYAPYARRIFGKTAEMLDFFSEKLGYPFVWEKYAQVCVHEYISGAMENATAVVHGTG
                     IQQDPRQQLDYDYEDVIAHELIHHWFGDLVTCASWSNVVLNEGFANYGEYLWREHAYG
                     SDDADRLNIEDLSSYLAATSKSDPPAVRYRYEDAEDLFDAVSYNKGGRILHLLRRQIG
                     DDAFFASLKLYLQRHAFGNAEVDDFRQAVEEVTGEDLHWFFDQWYFRPGRPILNVDYA
                     WDESARTMSVMLEQQQSMEEHAPYRLPMLIDVYTVNGRKSVPVDFNSAKQTIRIPLEQ
                     APELVNVDANKRILCTRRDNKPKEWLPYQYAHGPRYYDRWEAVSKLSENYEVGSAGGK
                     TIFSALQDPYWRIRKKAVESIGSWMQSRADTALPAVKRLATGDPESAVRSEAWRSLRR
                     FAEYDDYAELVPSGLRDSSYEVCAKVFELLKEKDPTLAAQQAPQLENDSGNAILEVMA
                     AYFATDEQSDRIGWYTRALRQAKGYT"
BASE COUNT         1039 a         1183 c         1190 g          990 t
ORIGIN      
        1 aagcgatgtc ataatcccag acgggtccgg ctttcagggt cttgttcttg tctttataca
       61 aataagaact gatacggtag ccatcaacat tcttgctcaa ctcattgcag aagaaataat
      121 cgatccagga accgtttcca atgtatttct ggtaaccgtt gatcgggtcc atgaaggtgg
      181 aagacgacaa cgcgtcctca aagctgtcga cataggcctg tatgtacgcc ttctgctgca
      241 gggaaatact gtccggatcc ggataatcat attgcagata ggtagtctga ccgttcgagt
      301 ggttgatcgg gggataaggg gaggtccaac cttcgccgcc acttccggtg gtcttatcga
      361 ccttaagaat atatccaccg gtaatgtccg gagcggtatt ttcccacgga tgcagtctgt
      421 cgatgtcgac ccggtctcca tcacgcttga tcgactccat cagcacgtag atgccgatgt
      481 actcaccgtt caggaccaca tcgacgtacc gcgtacgggc ggcgtaatgc cccatctcca
      541 ttgaaagttg gtacaagagt acgttccggc aaaacgattt gtcggtgtag ttggcactga
      601 ggatccaatc atgttccgac ggcatgccca gcagggcgac gttggtatcg gtaacaccgt
      661 tcggcatccg tgtttcaaag ccatacgact tcttggggaa cgactgcgag gacgatcccc
      721 ggatctcgat cgctatcttt ccgttgtatt cgttgaacgg atcgttcaaa tgattgcgtt
      781 ggccgatacc gttattcacg atacccatgt cgcagatgat cttcgggtcg tcgggaatcg
      841 actgcccgtt ggtgttgatg acaacgatgg gaagattgga accggaaaac agaaacggtc
      901 gtgcggggtt accgccaaaa ttgatactcc aattgatcaa tgagccggta atgccgttgt
      961 tgctgtcgtc gatcacacgt aactgccagg tgccgttacc gttttgcccg ttgttgacgt
     1021 tatacagggt tccttccgga cgatacgtgc cggtgaaagg cgcggagccg ctggtgatcg
     1081 acgtagcggc gtcgttgcgg aagatcgtgt tggtgaaatt ggaaccactc gttccgccat
     1141 tgtcggtggt caacatgata agcgtaccat ccggcgcggc caggaaacaa tcgatatcgc
     1201 gggcacggct gtgattgata ttgatcgtca ccgattcgag cccgaaggtg taatccacac
     1261 tggcgggaga gagtcccgaa acgttgatgt tgaagcggga agtatcggac aaggtgatga
     1321 tactgccacc ggtacccgaa aacgtttgcg cggatgcgaa acgcgtcagg gcgatgagtg
     1381 acagtcctaa gaacagccga agtttcatgc gagagggaca gaaaagttac tcaaaccgga
     1441 acgaatggta gatccggaga ctacaaggca aaaaccctgt ttctttaaaa ttttacgaaa
     1501 aaatgcagga aagttgtctg aaaaagggcg aaaatgtccc gtttttgcag tttagtgaag
     1561 cgaaaccatg acctggtttt tctccacttt atcgccttta ttgacgctga ttttccggat
     1621 agcgccgtca gccggagctt tgatgacatt ctccatcttc atggcttcca gcaccaggat
     1681 agggtcgccc tttttgattg cctgtccttc cgcaacagcg acattgagca ccagacccgg
     1741 catcggggct ttgatgtcat tcaccttttt gcctgcggtg atgtcgattc ccatctcgcg
     1801 cagcagatcg tcatacttgt cgcgcacatt gagctggtaa acggtattgt tcaccttgat
     1861 cgagagtgat ttctcctcga agttcgcttc gatcagttcg gcttcataag aacggccctt
     1921 gtacaggaga tgaaacttcc cttttcggta ttcgagaatg tcgggatgca tctcttcacc
     1981 gttcacgctt tggtggcggg catcgtacgc gacctggtat tctcgaccgt tgacggttac
     2041 cttgtacatg ggacggcaaa gttaacctaa aattcaagtc cgggccagcc cttatctttg
     2101 atgcaatgtc tgttcctgcg ccatttatcc ggctttccct gctgtccgtc gccctgttgc
     2161 tcgttgcctg ctcggggagc cggcaacttc cggcatcgcc tccggcagcc gccacctcaa
     2221 aaacggatcc tagaaaacag caggaagcct atgaggcaaa gccaaaccgg atttgggacc
     2281 tcgtacatac acgactcgag ttgcaacccc aatggggcag cagggagatc gacggtaaag
     2341 cgacgctctt tttgcatccg catttttacc cgaccgacag ccttgtactg gacgcgaaga
     2401 tgatgctggt aaaacaggta ggacgtatcc gtgcgcaagg cgatacgctg ctgataccgt
     2461 tccggtacga cagcagcacg atccggctga aactcgatac cgttttccat cgcgacaacg
     2521 agttggtgct atggatcgac tataccggcc ggccggacct gcgcacgggc gatggctcac
     2581 gggccattcg gggagagaaa ggtctttact tcatcaaccc cgacggaaag gatcccaaca
     2641 aaccgacaca attgtggagc cagggagaga cggcacataa ttccgtctgg ttccccacac
     2701 tggatgtgcc caatcaaaaa atgacccagg agatctatct cacggtggac acttccttcg
     2761 ttaccctttc caacggttta ttactctcgc agcaactgaa cggaaacgga acacgcaccg
     2821 attactggaa gcagagcctc ccggcgcccc cctacctcac gatgatcggc gtcgggcgtt
     2881 ggtcggttac gcgggaacgg tggaacaaca tcgaactgag ctactatacc gatcccgcct
     2941 acgcgcctta tgcccgccgc atcttcggaa agacagcgga aatgctcgac ttcttctctg
     3001 aaaaactcgg ataccccttc gtctgggaaa agtacgcgca ggtttgcgta cacgagtaca
     3061 tcagtggcgc catggaaaac gccaccgcgg tcgtgcacgg caccggcatc cagcaggacc
     3121 cacgacaaca actcgactac gactacgaag atgtgatcgc gcatgaattg attcaccact
     3181 ggttcggcga tctggtgacc tgcgcatcct ggagcaatgt cgtactcaac gaaggctttg
     3241 ccaattatgg cgaatacctc tggcgggaac acgcgtacgg cagcgacgat gccgaccggc
     3301 tcaacatcga ggacctctct tcttacctgg cggccacgag caaatcggat ccacccgccg
     3361 ttcgctatcg gtacgaggac gcggaggacc tgttcgacgc cgtatcctat aacaagggtg
     3421 ggcgtatcct gcacttgttg cggaggcaaa tcggcgacga tgcatttttc gcatccctga
     3481 aactttattt acaacgtcac gctttcggga acgcagaggt ggacgacttc cggcaggcgg
     3541 tggaagaagt caccggcgaa gacctccatt ggtttttcga tcaatggtac ttccgtcccg
     3601 gtcggccgat cctgaatgtc gactacgcct gggacgaatc agcccggacg atgagtgtga
     3661 tgcttgaaca gcaacaatcg atggaggaac atgctcccta tcgactgccc atgttgatcg
     3721 atgtctatac ggtgaacggc aggaagagtg ttccagtcga cttcaacagc gcgaagcaga
     3781 ccatccgcat tccgcttgag caggcacctg agctggtcaa tgtcgatgcc aataaacgca
     3841 tcctctgcac ccggcgagac aataaaccga aagaatggtt gccctatcag tatgcgcacg
     3901 gtccccggta ctatgaccgc tgggaagcgg taagtaaact cagcgaaaac tatgaagtcg
     3961 ggtccgccgg cggaaaaacc atcttctccg ccttgcaaga tccctattgg cgcattcgga
     4021 aaaaggcggt cgagtcgatc ggctcctgga tgcaatcgcg tgccgacact gcactacccg
     4081 cagtgaaacg acttgccacc ggcgatccgg agtctgccgt gcgatcggag gcctggcgca
     4141 gtttgcgccg cttcgccgag tacgacgact atgcggaact cgttccgtca ggtttgcgtg
     4201 acagcagtta tgaagtgtgc gcgaaagtgt tcgaactatt gaaagaaaaa gatcccacgt
     4261 tagccgcgca gcaggcgccg caactggaaa acgatagtgg caatgccata ctggaagtga
     4321 tggcggccta cttcgctacc gacgaacagt ccgaccggat cggctggtac acccgggcgt
     4381 tgcggcaggc gaaggggtat ac
//
LOCUS       DAOTON010000119         4398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16203_length_4398_cov_3.036150, whole genome shotgun sequence.
ACCESSION   DAOTON010000119 DAOTON010000000
VERSION     DAOTON010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16203_length_4398_cov_3.036150"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="P5292_03445"
     CDS             complement(<1..276)
                     /locus_tag="P5292_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRS38212.1"
                     /translation="MNPFEALLLCLVAFGGSLLTFFSGFGLGTLLMPVLAVFFPIEIA
                     IGMTAIVHLSNNLFKLALVGTKAKSDIILRFGIPSVLAALIGAFLLNF"
     gene            389..865
                     /locus_tag="P5292_03450"
     CDS             389..865
                     /locus_tag="P5292_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HRS38213.1"
                     /translation="MQDRPSFDEIYMDLAQNLARKSHCVKIKVGAVLTKDTRIVSLGY
                     NGPPAGTHNCDEEWPETGCPRDSKGSCSLALHAEQNAILYASKNNVSVDGCTLYITLS
                     PCLACARIIYTMGIKRVFYLQSYATYKGIGTDEGVDFLRRFGVEVRQYAPAPELNS"
     gene            913..2538
                     /locus_tag="P5292_03455"
     CDS             913..2538
                     /locus_tag="P5292_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRS38214.1"
                     /translation="MRSSRIWQPLLLAIMLAAGIYIGMRMSEPYRSSRGFFTFRTGQF
                     NKLNDVINYISNEYVDTVNSRALVESTIEDMLHQLDPHSSYIPGEDLQAMNEPLEGNF
                     DGIGVEFHIQDDTIMVVSALAGGPSEQLGIQAGDRIIRVEGKNVGGIKITNTQVMQLL
                     RGPSGTMVKVTILRRSTGQTIDFTIKRGKIPIYSVDVGYMLNRETGYIKIGHFADKTY
                     DEYLKAFADLKEKGMQNLILDLRGNPGGYLKTAIQIADEYLPDGKMIVYTEGRNRPKE
                     TFRATSRGFFEEGALVILIDEGSASASEIVSGAVQDWDRATIVGRRSFGKGLVQEQSE
                     FPDGSAVRLTVARYYTPTGRSIQKPYTAGYEQYETELLSRYQHGELLSQDSIKFADSL
                     RYTTPGGKIVYGGGGIMPDVFVPLDTAGTSDYYSEANGKGLINQFAYDYLDLHRKEIT
                     GYKDFDAFNQGFHVDDKIYNEFLTYARKSGMKADPAGARISSAIIRTQLKALIARQIW
                     KNDGFYPVIHSLDQTLRQALELIGKQQVAVKGS"
     gene            2644..3252
                     /locus_tag="P5292_03460"
     CDS             2644..3252
                     /locus_tag="P5292_03460"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRS38215.1"
                     /translation="MAFELPVLSYAFNALEPHIDARTMEIHHGKHHQAYVTNLNNAIA
                     GTDAEKLSIEEICRKISTLPVAVRNNGGGHYNHSLFWELMKPGGGALSGPLADAITST
                     FSSVDEFKSKFATAGATRFGSGWAWLVVTPEKKLAIGSSPNQDNPLMDISDLKGTPIL
                     GLDVWEHAYYLHYQNRRPDYISAWWNVVNWDEVARRFTAAMK"
     gene            3379..>4398
                     /locus_tag="P5292_03465"
     CDS             3379..>4398
                     /locus_tag="P5292_03465"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRS38216.1"
                     /translation="MQFARSRLLRIFLRLLLVAGGAFLALVLVVTVFYEAEVKQKIIS
                     ELNRHLRSEIKVSDFDISLLRHFPFASFEMKEVTAMDAVAGKESDTLLRAERISLQFN
                     LAGLLRKDIVIRRITANDGFIRLRIDEQGEPNYIIWKTDSTAATGSGGIDLSLVHLLR
                     MDVRYDDRKNRQDYDFNFRDARIAGKFSSDRFNLKAEGDLLCRRFVSDSTVYLKEKAV
                     RLEGALDVNTTTGQYRLEPTRLKVAAVDFNVGGDFTVDSVATKLNLTVEASEAGLKEF
                     ISLLPAGYEVYFKDYKTSGRFLFRGTIRGTANKQSSPAIVVDFSIRDGRLQPKGEDVA
                     LERIQF"
BASE COUNT         1069 a         1179 c         1180 g          970 t
ORIGIN      
        1 gaaattcaag aggaacgcgc cgatcaaggc ggccaatacg gacgggattc cgaaacgaag
       61 tatgatatca cttttcgcct tggtaccgac aagcgcgagt ttgaacaggt tattgctcag
      121 gtgtacgatt gccgtcatcc cgatggcgat ctcgatcggg aagaatacgg ccagcaccgg
      181 catcaggagt gtacccaggc cgaaaccgga aaagaaggtc agcaggctgc cgccaaaggc
      241 taccaggcaa agtagaagag cttcgaatgg attcatcgcg cagcaatcgg atcaaggccg
      301 aaaccggatt taaactggta gtacggggat tgtcctattc cgttacagcc ggaaagtcga
      361 aaggtggaat tctcgctatc tttacagcat gcaagaccga ccctcgtttg atgagatcta
      421 tatggatctg gcccagaacc tggcccgtaa gtcgcattgc gtcaagatca aagtcggtgc
      481 cgtattgacc aaagacaccc gcatcgtttc gctgggttac aatggtccgc ctgccggaac
      541 ccataattgc gacgaagaat ggccggagac cggctgtccg cgcgacagca aaggttcctg
      601 ttccctggcc cttcatgccg aacagaatgc tatcctgtac gcgtccaaga acaatgtttc
      661 agtggacggt tgtacgttgt acatcaccct gtcgccctgt ctggcttgtg cccggatcat
      721 ctataccatg ggcatcaagc gggtctttta cctgcagtcc tacgcgacct ataagggcat
      781 cggaaccgac gaaggcgttg atttcctgcg tcgattcggg gtagaagtgc ggcaatatgc
      841 accagccccg gaactcaata gttgaacctt tttccgcgtt ttacgttatc gctcatttac
      901 aagtaatcat ccgtgcgtag ttccaggatt tggcaaccct tactgctggc gatcatgctg
      961 gcggccggca tatatatcgg tatgcggatg agtgaaccgt atcgcagctc gcggggcttt
     1021 ttcacgttcc ggaccgggca attcaacaag ctcaacgatg tgatcaatta catctcgaac
     1081 gagtacgttg acacggtgaa ctcccgcgcg ctggtcgaaa gtacgatcga ggatatgctg
     1141 caccagctcg acccgcattc ttcttacata ccgggcgaag acctgcaagc gatgaatgaa
     1201 ccgctggaag ggaacttcga cggaatcggc gtcgagtttc acattcagga cgatacgatc
     1261 atggtcgttt ccgcccttgc gggaggacct tcggagcaac tgggaatcca ggccggtgac
     1321 cgcatcatcc gtgtcgaagg aaagaatgtg ggcgggatca agatcacgaa cacccaggtc
     1381 atgcaattgt tgcgcggacc aagcgggacc atggtcaagg tgacgattct ccgtcgttca
     1441 accggacaaa cgatcgattt caccatcaag cgtggtaaga ttccgatcta cagtgtcgac
     1501 gtaggctaca tgctgaaccg tgaaaccggt tatatcaaga tcggccactt cgcagacaag
     1561 acctatgatg aatacctcaa ggctttcgcc gacctgaagg agaagggtat gcaaaacctg
     1621 atcctcgatc tgcgcggaaa tccgggagga tatttgaaaa cggccatcca gattgccgat
     1681 gaatatctcc cggacggaaa aatgatcgtt tacaccgaag gaaggaaccg tcccaaggaa
     1741 accttccggg caacctcccg cggtttcttt gaagagggcg cgctggtgat cctgatcgat
     1801 gaaggcagcg cttcggcgag tgagatcgta tccggagccg tacaagactg ggaccgtgcc
     1861 accatcgttg gccgtcgttc cttcgggaaa ggcctggtcc aggagcaaag cgaatttccc
     1921 gacggctcag cggttcggct caccgtcgca cgctattata caccgaccgg acgtagcatt
     1981 caaaagccct acacggccgg ttatgagcag tacgagaccg aactgctttc gcggtaccag
     2041 catggagaat tgctctcgca ggatagcatc aaattcgcgg attctttaag atatacaaca
     2101 cccggcggga agatcgtcta cggtggcggt ggcatcatgc cggatgtatt cgttccactc
     2161 gacactgccg gaacatccga ttactacagt gaagcgaacg gtaaggggct gatcaaccag
     2221 tttgcctacg attacctcga cttgcaccgc aaggaaatta ccgggtacaa ggatttcgac
     2281 gccttcaacc agggtttcca tgtcgatgat aagatctaca acgaattcct gacctatgcc
     2341 cggaagtccg gcatgaaggc ggaccctgcc ggggcgcgta tttcatcggc catcatccgg
     2401 acgcagctca aggccctcat tgcccgacag atctggaaga acgatggctt ttatccggtt
     2461 atccactcgc tggaccagac acttcgacag gcactcgaat tgatcggcaa gcagcaggta
     2521 gcggtcaagg ggagttgatt tttctgcctt gtattggcag gatattactg gttcatttct
     2581 cgtatttttg catcaggaaa tcatccggat ttcccgttca actattgtta aaaaagacac
     2641 atcatggcat tcgaacttcc cgtcctttct tatgcgttca acgcgctgga gccgcacatc
     2701 gatgcgcgta ccatggagat ccaccacggt aagcaccatc aggcttatgt aaccaatctc
     2761 aataacgcga ttgcaggaac cgatgcggag aaactcagca tcgaagagat ctgccgcaag
     2821 atttcgacac ttccggtcgc ggtacgcaat aacggcggcg gccactacaa tcacagtctc
     2881 ttttgggaac tgatgaagcc gggtggcggc gcgctctccg gcccgctcgc ggacgcgatc
     2941 acaagcacgt tctcttcggt ggatgaattc aaatccaagt tcgccacggc cggcgcgaca
     3001 cgtttcggaa gcggttgggc ctggctggtc gtaacgcccg agaagaaact ggccattgga
     3061 tccagcccga atcaggacaa ccccttgatg gatatttcgg atctcaaagg gacgccgatc
     3121 ctcggactcg acgtttggga acatgcttac tacctgcatt accagaaccg cagacccgat
     3181 tacatctccg cctggtggaa tgtggtcaac tgggacgaag tcgcccgtcg tttcactgct
     3241 gcgatgaagt gattattcca gcaaacttgc tttaaaaagc gaacccgagg gttcgctttt
     3301 tttgtccccg ggataacaat tcccccggct ccccggcgtt atgcaacctt tgaaggatgt
     3361 cttcgttcat ccgatcggat gcagttcgcc cgctctcgcc tgttgcgcat attcctccgc
     3421 ctgctgctgg tagcaggtgg cgcattcctg gcgttggtgc tggtggtcac cgtattttac
     3481 gaagcggagg ttaagcagaa aatcatttcg gaattgaacc ggcatttgcg gtcggagatc
     3541 aaagtaagtg atttcgatat ttccctgctg cgccacttcc cttttgcttc ctttgaaatg
     3601 aaggaggtga cggcaatgga cgcggtcgcg gggaaagaaa gcgacacctt gttgcgggcc
     3661 gaacggatct ccctgcagtt caacctggcc ggtctgttac gtaaagacat tgtcatccgc
     3721 cgcatcaccg ccaacgacgg attcatcagg cttcggatag acgaacaagg ggaaccgaat
     3781 tacattatct ggaaaacgga ttcaacggcc gcgaccggca gcggtggaat cgatttatcc
     3841 cttgtgcacc tgctgcgcat ggatgtgcga tacgatgatc gaaagaaccg gcaggattac
     3901 gacttcaatt tccgcgacgc ccgcattgcg ggtaaattct cctccgaccg cttcaacctg
     3961 aaagcggaag gtgacctgct ttgcagacgg ttcgtatccg actcaacggt ttacctgaaa
     4021 gagaaagcgg tgcgcctcga gggagcactt gatgtaaaca cgaccaccgg acaataccgg
     4081 ctcgaaccga cccggctcaa agtcgcggcg gtcgatttca atgtcggcgg agatttcacc
     4141 gttgactccg ttgccacgaa actcaacctg acggtagaag caagcgaagc gggactgaaa
     4201 gaattcatca gcctgttgcc tgccgggtac gaggtctatt ttaaggatta caagacgtcg
     4261 ggccgttttc tattccgggg aacgatccgt ggaactgcca acaaacaatc atcccccgcc
     4321 attgtggtcg acttttccat ccgagacgga agattgcaac caaagggaga agacgtggca
     4381 ctcgagcgga ttcaattc
//
LOCUS       DAOTON010000120         4375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16297_length_4375_cov_3.160880, whole genome shotgun sequence.
ACCESSION   DAOTON010000120 DAOTON010000000
VERSION     DAOTON010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16297_length_4375_cov_3.160880"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1064
                     /locus_tag="P5292_03470"
     CDS             204..1064
                     /locus_tag="P5292_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS38217.1"
                     /translation="MFYTTALVLGLAFGAMALGVFLSMRIFSIPDITTDGSFTLGGAV
                     TAIGLVNGWSAVALLPVALVAGGLAGAATGWIHTRLKVHALLAGILVMTGLYSINLAI
                     LGRPNVPLLGTDSLFLLFPSTSNESLRQLWVLTIVVAALWFGLRWLLRTDFGLSMRAT
                     GNNETMIRALGVNTDGRKILGLAFANGLTALSGYLIVQVQGFADINMGIGIVILGLGA
                     VMIGESLLRGGIRSRIGWQLASVIAGSLVFRLLLAFALSMGIDALWLKLVVAVFVLIV
                     IALPKLKTAR"
     gene            1061..1813
                     /locus_tag="P5292_03475"
     CDS             1061..1813
                     /locus_tag="P5292_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS38218.1"
                     /translation="MIRLEHIRCSFPSGQGERTLALDGIELIVPEAQFITVIGTNGSG
                     KSTLLNCIAGTVQPQSGRVLFDGTDVTDLADHQRSRWVARIFQNPLAGTAPELSLLEN
                     FRLAALRTQPKGFRIGTDQAFRKKVEQAVEEIGLGLERKLDQPMGTFSGGQRQALTLV
                     MAVMDRSSILLLDEPTAALDPRTASLVMRLADQLIRKHRLTALMVTHNLKDALHYGDR
                     LLHLAEGRIAHDLTGKEQESLSTTTLLDWFEA"
     gene            1899..3920
                     /locus_tag="P5292_03480"
     CDS             1899..3920
                     /locus_tag="P5292_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRS38219.1"
                     /translation="MRRLYLLLSLLAFCATANAQILKPAKWKFTVEPGKSGEQTLVFS
                     AKLDEHWHMYSLYTPDGGPLPMVITYEQSNCFKTIGKAVEYKPVEEYDSVFMVKVLIF
                     HHTAEIRQKVKVTGDCVIKGRIEYQVCKESCIFQEDEFEFKVSANTEEGKANTETETQ
                     ASVPVTPVDTPAVQALDTAAGTITAPASTDSKLESGCGAGETPPAGGASTPWTIFIAG
                     MLGGLLALLTPCVFPMIPMTVSFFTKRSGNRAKGVRNALIYAISIILIYVTLGLLVTV
                     TFGSDALNALASNAFFNLAFFIIFIVFAISFFGAFEITLPSWIINKADSASDRGGLIG
                     IFFMAFTLSLVSFSCTGPIIGTLLVEAAHGRSYLGPLMGMTGFSFALAVPFALFALFP
                     SWLNSLPKSGGWLNTVKVSLGFIELALALKFLSNVDLAYHWGLLKRELFIVLWIVIFG
                     MLGLYLLGKIRLSHDSDQQHVGIGRLLFALLSLSFALYLVPGIWGAPLKLISGFPPPD
                     FYKEWKTGKEGDCPHDILCFKDYEEGMRYAKEHNKPVMIDFTGWSCVNCRKMEDNVWS
                     DPKVLKKLGEDYVLISLYVDDKTPLPTEQQSVSPTTGRKIRTTGNKWSDLQASVYNTN
                     SQPYYVLLDHQGKILAEPRGYTPDISAYLSFLEEGLCRYQQRKSTLAAN"
     gene            4003..>4375
                     /locus_tag="P5292_03485"
     CDS             4003..>4375
                     /locus_tag="P5292_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRS38220.1"
                     /translation="MQRKFVTNLALVLALNLLIKPFWILGIDRAVQNAVGTEQYGFYY
                     AIFNFSFLLNILLDLGITNFNNKNIAQNNHLLSKHFSSIVMLRVLLAGVFTLATMVGG
                     FIIGYTADMLKLLLAVLVNQIL"
BASE COUNT          920 a         1266 c         1239 g          950 t
ORIGIN      
        1 cgatggccgc acgcatgttg aagcagggaa acgctgacgg actcaagccg gaagcattga
       61 gggtgcggaa gcgggtggtg aatgaggaga aactaaaaag cctcggcgtt gctttacaat
      121 aatggcacgc ggataacacg gacaggcacg gaaattcacg gatacgtcgc atacaacttt
      181 caagaatcca gtctcaccga ttcatgttct acaccaccgc cctcgtactc ggtctcgcat
      241 tcggcgccat ggcgctgggg gtgtttctga gcatgcggat cttttcgata cccgacatca
      301 cgaccgacgg cagttttacg ctgggcggag cggtgacggc gatcggactg gtgaatggct
      361 ggtcggcggt tgcgctcttg ccggtcgcgc tggtggcggg cggactcgca ggcgccgcga
      421 caggatggat ccacacgcgt ttgaaagtac acgcgctgct ggccggcatc ctggtgatga
      481 cgggcctcta ctccatcaac ctcgcgatcc tgggtcgtcc gaacgtgccg ctgctgggca
      541 ccgatagttt attcctgctc ttcccttcca ccagcaatga aagcctgcga cagctttggg
      601 tcctgacgat cgtcgtcgcc gcattatggt tcggcttgcg ctggctgctg cgcaccgatt
      661 tcggtttatc gatgcgcgcg acgggtaaca acgaaaccat gatccgcgcg ctgggggtga
      721 atacggatgg acgcaagata ctcgggctcg ccttcgcgaa cggattgaca gcgctcagcg
      781 gttatctgat cgtgcaggta cagggatttg ccgacatcaa catgggcatc gggatcgtga
      841 tcctgggcct gggtgccgtg atgatcggag aaagtttact acgcggcggc atccggagca
      901 ggatcggctg gcagttggca tcggtgatcg cgggcagcct ggtcttccga ctgctgctgg
      961 cgttcgcgct cagcatgggc attgacgcgc tctggctgaa gcttgtggtc gcagtgttcg
     1021 tgctgatcgt cattgcactt cccaaactca aaaccgcccg atgatccggc ttgagcatat
     1081 tcgctgcagt ttcccgtccg gacagggcga acgaacactc gcgctcgacg gcattgagtt
     1141 gatcgtacct gaagcgcagt tcataacagt catcgggacg aacggctccg gcaagtccac
     1201 cctgctcaat tgcatagccg gaactgtaca gccccaaagc ggccgggtac tcttcgacgg
     1261 caccgatgtc accgacctgg ccgatcatca acgctcgcgt tgggtcgcgc ggattttcca
     1321 gaatccattg gccggcaccg cgccggagct cagcttattg gagaactttc gcctcgcggc
     1381 actgcgcaca cagcccaagg gtttccgcat cggaaccgat caggcgttcc ggaaaaaagt
     1441 cgagcaagcg gtcgaagaga tcggactggg gttggaacgc aagttggatc aacccatggg
     1501 aacgttttcg ggcgggcagc ggcaagccct gacgctggtc atggccgtaa tggaccgatc
     1561 gagtatactc ctgctcgacg aacccacggc ggccctggac cccagaacgg cgagtctggt
     1621 catgcggctg gccgaccaac tgatccggaa acatcgtttg acggccctga tggtgaccca
     1681 caacctgaag gacgccctgc actatggaga ccggctgctg caccttgcgg aaggccgaat
     1741 cgcgcatgat ttgaccggaa aagagcagga aagcctgagc acaaccacgc ttttggactg
     1801 gttcgaagcc tgaaatttta ctattttcgc ctttcacgta cacgattcct tcacggggca
     1861 cgttgtacat tttgctcccc ttgccgggac aatcaactat gagaagactc tacctcctgc
     1921 tttccctgct ggcattttgc gcaactgcga acgcgcaaat cctgaaaccg gctaaatgga
     1981 aattcactgt cgagccgggt aaatcgggcg aacagacgct ggtcttttcc gccaagctgg
     2041 acgagcactg gcatatgtac agtctctaca cgccggatgg cggaccgctg ccgatggtga
     2101 tcacctacga acagagcaat tgcttcaaga cgatcggcaa agcggtggag tacaaaccgg
     2161 tggaagaata cgactccgtg ttcatggtga aggtcctgat cttccatcac acggcggaga
     2221 tccggcagaa ggtgaaagtg accggtgatt gcgtcatcaa aggacgcatc gagtaccagg
     2281 tttgcaaaga gagctgcatc ttccaggaag acgaatttga attcaaggtt agcgcgaata
     2341 cggaagaagg aaaagccaat accgaaaccg aaacgcaggc cagtgttccg gttacaccgg
     2401 tcgacacacc tgccgtccag gcgttggata cggcagccgg caccatcacc gcaccagcgt
     2461 cgaccgacag caaactggaa tcaggatgcg gcgcaggcga aacccctccg gcgggcggag
     2521 cctccacacc ctggaccatc ttcatcgcgg gcatgctggg aggcctgctg gccctgctga
     2581 caccctgtgt gtttcccatg atcccgatga cggtgagttt ttttaccaag cgcagcggca
     2641 accgggcgaa gggtgtccgc aacgcgctga tctacgcgat ctcgatcatc ctgatctatg
     2701 tcacgcttgg actgctggtg accgtcacct tcggctccga cgcgttgaac gcattggcca
     2761 gtaacgcttt ttttaacctg gcgttcttca tcatcttcat tgtcttcgcc atctccttct
     2821 tcggcgcgtt cgagatcacg ctgccgagct ggatcatcaa caaagccgat tccgccagtg
     2881 atcgcggcgg actgatcggc atcttcttca tggcattcac gctctcgctc gtctccttca
     2941 gttgtacggg cccgatcatc ggcaccttgc tggtggaagc cgcgcacggc cgcagttacc
     3001 tcggcccact catgggcatg acgggatttt ccttcgcgct tgcggtccct ttcgccttgt
     3061 tcgccttgtt tccgagctgg ctgaattcct tgccgaaatc cggcggctgg ctgaacaccg
     3121 tgaaagtgtc gctcggtttt atcgaactgg cgctcgcgtt gaagtttctc tccaacgtcg
     3181 acctggccta tcactggggc ctgctcaagc gcgagctgtt catcgtcttg tggatcgtga
     3241 tcttcggcat gctgggcctc tacctgctcg gaaagatccg actctcccac gacagtgacc
     3301 agcagcacgt cggcatcggt cgcctcctgt tcgcgctctt gtccctgagc ttcgccttgt
     3361 acctcgtacc cggcatctgg ggagcgccct tgaagctgat cagcggattt ccgccgcccg
     3421 atttctacaa agaatggaaa accgggaagg aaggcgattg tccgcacgac attctttgct
     3481 tcaaggatta tgaagaaggc atgcgctacg cgaaggagca caacaaaccg gtcatgatcg
     3541 acttcaccgg ctggagttgt gtcaactgcc ggaagatgga agacaacgtc tggagtgatc
     3601 cgaaagtgct caagaaactg ggtgaagatt atgtcctgat ttccctctac gttgacgaca
     3661 agacaccctt gccgaccgaa cagcaatcgg tctccccgac caccgggcga aagatccgca
     3721 ccaccggaaa caagtggagc gacctgcagg cttcggtcta taacaccaac agtcagcctt
     3781 attatgtcct gctcgaccac cagggaaaaa tcctggcgga accgcggggt tacacacccg
     3841 acatatccgc ttacctctct ttcctggagg aaggcttgtg ccgttaccag cagcggaaat
     3901 cgacgctggc ggcgaactga ttaccggtca tcgatttttt ccgcttgcag tgcggtaggc
     3961 tgacccgccg ggatgccgta cctacgcagc cgtttttagt gtatgcaacg taagttcgtc
     4021 accaacctcg ccctggttct ggccctgaac ctgctcatca agccgttctg gatcctgggc
     4081 atcgaccgcg cggtacagaa cgcggtgggg acggaacaat acggtttcta ttatgccatt
     4141 ttcaatttct cgttcctgct gaacatcctg ctggacctgg gcattacgaa tttcaacaac
     4201 aagaatatcg cccagaacaa ccacctgttg tcgaagcatt tttcgagtat cgtgatgtta
     4261 cgggtattgc tcgcaggcgt tttcacgctg gcgacgatgg tgggcggttt catcatcggc
     4321 tatacggccg acatgctgaa gctgctgctg gcagtgctgg tcaaccagat cctga
//
LOCUS       DAOTON010000121         4374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16302_length_4374_cov_3.609169, whole genome shotgun sequence.
ACCESSION   DAOTON010000121 DAOTON010000000
VERSION     DAOTON010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16302_length_4374_cov_3.609169"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1067)
                     /locus_tag="P5292_03490"
     CDS             complement(138..1067)
                     /locus_tag="P5292_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS38221.1"
                     /translation="MNILTIEHVSKSYAAHKALDNVSLQVPKQSIFGLLGPNGAGKTS
                     LIRIITQITAPDSGRILFEDKPLSEEHIARMGYLPEERGLYKKMEVGEQALYLARLKG
                     LSRADAMKRLKYWFEKFEIQAWWKKKVEELSKGMAQKVQFITTVLHEPSFLILDEPFT
                     GFDPINAELVKNELLELKSKGTTIILSTHRMESVEELCSHIGLINRSQLVLQGPVRDI
                     RKAHKSNTYELSFRGNRISFTHALWTGFELLDVKEEDDHQVAFVKMLDGHTPNQLLQL
                     LLPNVEVLGLREIIPTMSDIFIQKVGASNQEIH"
     gene            complement(1185..2342)
                     /gene="dnaJ"
                     /locus_tag="P5292_03495"
     CDS             complement(1185..2342)
                     /gene="dnaJ"
                     /locus_tag="P5292_03495"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRS38222.1"
                     /translation="MAKRDYYEVLGVQKSSSEADIKKAYRQMAMKYHPDRNPGDKEAE
                     EKFKEAAEAYEVLSDTDKRKRYDQFGHQGMGGNGGFGGGGGMSMDDIFSHFGDIFSEG
                     SPFESFFGGGGGGGRGRRVNRGSNLRIKVKLTLEEIAHGVEKKVKVAKYVGCGTCHGS
                     GAAEGASFNRCTTCNGSGQVHRVTNTFIGQMRTTTTCPSCNGEGQTIANKCKTCSGAG
                     IMKGEEVISINIPPGVGEGMQLSMSGKGNAGERGGIPGDLIIVIEEVPHQHLQRDGSN
                     LLFDLYVNFADASLGTAVEIPTLEGKAKVKIEPGTQAGKVLRLKGKGLPSVNAYGRGD
                     LLVNINVWTPQHLSSEEKKLLEKLRESPNFRPNPGKNDKSFYEKMREFFHS"
     gene            complement(2375..2950)
                     /locus_tag="P5292_03500"
     CDS             complement(2375..2950)
                     /locus_tag="P5292_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRS38223.1"
                     /translation="MKTNSEHEATEGKPMDDGKKTAESAPESVNEAALPDAAVEQMDP
                     VAHLEASLSEAQDKYLRLYSEFENYKKRVARDRVDQSRMAAADTWSAILPVIDDFERA
                     IKAMESKAAADPALDGIRLIYSKLKSITEARGLKPMDAIGKPFDPELHDAITRMPAPD
                     DSMKGAVLDEVEKGYFLHDRVLRHAKVVVGQ"
     gene            complement(3122..3559)
                     /locus_tag="P5292_03505"
                     /pseudo
     CDS             complement(3122..3559)
                     /locus_tag="P5292_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020597426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
     gene            complement(3731..>4374)
                     /locus_tag="P5292_03510"
     CDS             complement(3731..>4374)
                     /locus_tag="P5292_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRS38224.1"
                     /translation="IQGVSSLRGTEHKLLPDMIEIGSFIGLAAMTRSEITIKEVSYPN
                     LGIIPTVFERLGIRMELRGDDLYIPKHDHYEIETFIDGSILTVADQIWPGFTPDLLSV
                     ILVTATQAAGTVLVHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLNRKVPLRAI
                     QMTSPDIRAGVALLIAAMSAKGKSVIHNIEQIDRGYQRIDERLNAIGAQIRRV"
BASE COUNT          915 a         1215 c         1098 g         1146 t
ORIGIN      
        1 ccacccatca ggagcggacc gaggatcgtc atgaccagga atgatttctt ccggacgcgg
       61 gtcaggtatt cccgttggat gaccagtagt actttggaca tggcgctact tgctttcgtt
      121 taaatcgttg aatcggttca atgaatttcc tggttggaag ctccaacctt ctggatgaag
      181 atatcgctca tggtcgggat gatctcgcgc agtccgagga cctccacatt cggcaacaac
      241 aattgcaata gttggttcgg ggtatgccca tccagcatct tgacaaatgc aacctgatgg
      301 tcgtcctcct ccttcacatc gagcagctcg aaaccggtcc aaagcgcgtg cgtgaaagaa
      361 atgcgattac cccggaacga aagttcatag gtgttgctct tatgcgcttt gcggatatcg
      421 cgtacagggc cctgcaagac cagttgagaa cgattgatca gtccgatatg gctacacagt
      481 tcctccacgc tttccatacg gtgcgtggaa aggatgatcg ttgtcccttt ggatttcagc
      541 tccagcagct cgttcttgac cagttcggca ttgatgggat cgaagccggt aaagggctcg
      601 tcgaggatga ggaagctcgg ctcgtgcaat acggtggtga tgaactgcac cttctgggcc
      661 atccctttcg agagttcctc gactttcttt ttccaccagg cctgtatctc gaacttttcg
      721 aaccagtatt tcagtcgctt catggcatcc gcccggctca gccctttcag tcgggccaga
      781 tagagcgcct gctctccgac ttccattttc ttatacaatc cccgctcctc cggcaggtat
      841 cccatccgcg cgatatgttc ttccgagagt ggcttgtcct cgaacaagat tcgtccactg
      901 tcgggcgcgg tgatctgcgt gatgatccgg atgagtgagg ttttaccggc accgttgggg
      961 ccgagcaggc cgaagatgct ttgcttcggt acctggaggc tgacattatc cagcgccttg
     1021 tgggccgcat aggacttgct gacgtgctcg atcgttaaaa tattcataag aatccggcga
     1081 ttattgacct tctggaaacc ggatacgaag aacgcaggat cggaggactt tcacaataga
     1141 aaaggccgct tgtccggcgg ccttcatctt ttctttcagc atgatcagga atggaaaaac
     1201 tcccgcatct tctcgtaaaa actcttatcg ttctttcccg ggttggggcg gaagttcgga
     1261 gactcccgaa gtttctccag cagcttcttt tcttcagaag acaagtgttg cggcgtccag
     1321 acattgatgt tcacgaggag gtcgccgcgg ccatacgcat tcaccgaggg caggcctttt
     1381 cctttgagtc gcagcacttt cccggcctgg gtgcccggct cgatcttcac cttggctttt
     1441 ccttccagtg tcgggatctc gaccgcggta cccagggaag cgtcggcgaa attgacgtag
     1501 agatcgaaca acaagttact gccgtcgcgt tgtaaatgct ggtggggcac ctcttcgatc
     1561 acgatgatca ggtcgccggg aattcctccg cgttctcccg cattgccctt cccgctcatg
     1621 ctcaactgca tgccttctcc cactccgggc ggaatattga tggagatcac ttcttccccc
     1681 ttcatgatac cggcacccga acaggtttta cacttattgg cgatcgtttg accttcaccg
     1741 ttgcaagacg gacaggtagt cgtcgtccgc atctgcccga tgaaggtgtt ggtgacacga
     1801 tgtacctgac cactgccgtt acaggtcgta cagcggttga aggatgcgcc ttcggcagcg
     1861 ccgctgccgt ggcaggtgcc acagccgaca tacttcgcca ccttaacttt tttctcaaca
     1921 ccatgcgcga tctcctccag cgtcaatttc actttgatcc gcaggttgga tccgcggttc
     1981 acacgccgcc cacgaccgcc gccgccacct cctccaaaga aactttcgaa cgggcttcct
     2041 tcactaaaaa tatctccgaa gtgtgagaaa atgtcgtcca tcgacattcc accaccacca
     2101 ccgaacccgc cgtttccgcc cattccctga tggccgaatt ggtcgtagcg ctttcgctta
     2161 tccgtatcac tcaggacttc gtaagcttcg gcagcttctt tgaacttttc ttccgcctct
     2221 ttatcgccgg ggtttctgtc gggatggtat ttcatggcca tctgacggta agcctttttg
     2281 atatcggctt ccgacgagga cttctgtact cccagaactt cgtagtaatc tcttttggcc
     2341 atgtcttaaa cgcgaatggt tctcaattat aggatcattg accgaccacc actttcgcgt
     2401 ggcgcaatac ccggtcatgc agaaagtaac ccttctccac ttcgtccaac acggcgcctt
     2461 tcatgctatc atccggagct ggcatacggg taatggcgtc gtgtaattcc ggatcaaacg
     2521 gttttccgat cgcatccatc ggcttcaggc cacgcgcctc cgttatggat ttcaatttgg
     2581 aatagatgag gcgaatacca tccagtgccg gatcagcggc agccttggac tccatcgctt
     2641 tgatggcacg ctcaaagtca tcgatcaccg gcaggatggc cgaccaggta tcggcagcag
     2701 ccatccggct ttgatcgacc cgatcacgcg ccacccgctt cttataattc tcaaactccg
     2761 aataaaggcg gaggtattta tcctgcgctt cggagagact cgcttccaag tgcgccaccg
     2821 ggtccatttg ttcgaccgcg gcgtccggca atgcggcttc gttgaccgat tctggtgctg
     2881 actcggcggt cttttttcca tcatccatcg gctttccctc cgtagcttcg tgctccgaat
     2941 tggtcttcat gtcagctatc ttttgttttt attagcaaac agccctttta caaggatgct
     3001 gccactggtc gagccaggcc aaattggcag tattcccctt taaacggctg caaagttcgc
     3061 cattttctaa aaaaaaagag gccaaatcat ttggcctctt tcagcatgaa ctctggttca
     3121 attatttcac ttccagcttt tccaactctt gagaaagagc ggcgccacgg agattcttcg
     3181 ctacgatgat gcctttgtcg tcgatcaacc aattggtcgg gatggcattc acgccatagt
     3241 ttttcgctgc gtcagaatac caccacttaa ggtcggagac gtgattggtc cattccaaac
     3301 catcctggcg aattgcgttc atccaggcgg ttttgtcctt gtcaagcgac acggaataga
     3361 cggtaaatcc ttttcctccc ttgaatttgg aagccttgaa gcgcttatag gtctgtacaa
     3421 cgttagggtt ttccatccgg caaggtccgc accagcttgc ccagaagtcg atcaggacca
     3481 ttttaccctt cagggatgat agcggaatta cgttgccttc cggtgagctg agtttgatgt
     3541 ccggagcgag gtttcctact tccgtaccgg tcttcgggct ctgggcattt gcgaccaaga
     3601 ccgaaaagaa catgatgagg gcaaataagc ctgatttttt cattttttgg gtttttcgtg
     3661 gtgcattttt ggcaaatacc ctgccaagtt aatcaatttc gggtctgtac gcacttaggg
     3721 tttaggtggt tcagaccctg cggatctgtg caccgatcgc gttgagacgt tcatcgattc
     3781 gttgatagcc ccggtcgatc tgctcgatgt tgtggatgac gctctttcct ttggcagaca
     3841 tggccgcaat caacaaagca acacctgccc ggatatcagg cgacgtcatc tgtatcgcgc
     3901 gaagcggcac cttacggtta aggccgatca cggtagcgcg gtgcggatca caaaggatga
     3961 tctgcgctcc catatcgatg agcttgtcga cgaagaacaa ccggctctcg aacattttct
     4021 ggtgcacgag caccgttcct gctgcctgcg tcgcggtgac gaggatcacc gacaacagat
     4081 cgggtgtaaa gccaggccag atctggtcgg ccactgtcag gatcgaaccg tcgatgaagg
     4141 tttcgatttc gtagtggtcg tgctttggaa tgtagaggtc atcgccgcgg agttccatcc
     4201 ggatacccag gcgttcgaac acagtgggga tgattccaag attcgggtag ctgacttctt
     4261 tgatcgtgat ctccgaacgg gtcatcgcgg ccaagccgat gaacgaacct atttcgatca
     4321 tatcgggcaa caacttgtgt tcagtaccgc gcagactact cactccctgg atgg
//
LOCUS       DAOTON010000122         4354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16393_length_4354_cov_3.774599, whole genome shotgun sequence.
ACCESSION   DAOTON010000122 DAOTON010000000
VERSION     DAOTON010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16393_length_4354_cov_3.774599"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="P5292_03515"
     CDS             <1..304
                     /locus_tag="P5292_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38225.1"
                     /translation="SGQAGSDSITVIFDVCDQVGTPLGDRIALYPVPSTGRINISESP
                     VPYRLVITDLTGRAVWVESVPAGNVKQLKLPSGVFHFLLQTEQQEILLSRKVVVLE"
     gene            complement(325..816)
                     /locus_tag="P5292_03520"
     CDS             complement(325..816)
                     /locus_tag="P5292_03520"
                     /inference="COORDINATES: protein motif:HMM:NF028004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HRS38226.1"
                     /translation="MNNSLKLMLMLLPAVTTMIACKKDKEESSADQAFSSEIRNGSYS
                     YYQNGNTLSAASPSPHGSFKLKFNSTALAALDSTGELPSGATFPNGSVIVKEVVSGSG
                     VTLLAVMKKEPSNANAGSGWMWAEYEPDGSTVFSVGKKGDGCIGCHSGSPNRDLVRSF
                     DLH"
     gene            complement(965..2185)
                     /locus_tag="P5292_03525"
     CDS             complement(965..2185)
                     /locus_tag="P5292_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38227.1"
                     /translation="MRLTLLLLIWSLFASAQQTFTVFDRNNSPLPDNQCRYIAIDSLN
                     RKWFCTEFGLAIYDDNTWTVYQTTNSGLTDNSVKHVAFDAAGRAWISTQNGGVCVFDG
                     TNWTSYTTANSPIAADYIRSVTIDRFGSAWICTIAGLSRFDGANWTTWNIGNSNIPVD
                     HISRCVIENDTLRWLGTVNGGFSQMIRDTGFTTWDYMHQNFPDNSMNDLVLGPLNHLW
                     MTCPAGALVNFYNGGWFTYNTLSSGIPTNSLNSLQFDSLTNIWLGTYDRGIVKKEGPV
                     YYSWYTANSDMPDDNVFYTCIEGNGIVWAGTEQSGVVRFDENLWLGPKPVAEPSRLTL
                     FPNPAHDFVNLRFDHARPDLVQLYNMQGQLIRSMHDLSAPVLRLDLTGLSSGCYLVRI
                     AEGTRIERKTLIIE"
     gene            2336..>4354
                     /locus_tag="P5292_03530"
     CDS             2336..>4354
                     /locus_tag="P5292_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRS38228.1"
                     /translation="MRPFLRLLTCLFCFLLSGARAQDPVYRVYDRRDGLPSNTVYCAF
                     QDARGFLWFGTDAGASRFDGRAFRGYNVQNGLVDSEVLRIYQDSKGRIWFLTLKGLLC
                     YYLDGKIHNPYNDPDLPKGLADRGLLSFREDAKGNIWFGGLSNQVLRIDPSDHCDFIM
                     PDTIANPARAGWTFLYEPEPGVMIVNVQDGLVRINSDDLKETPVEMPGIGDTVAWMEN
                     TGPRTGLLISTRGLFRLNGYEAELLLSAAEIPAQMNMGRITQDEDGSLWMMNNDKCVY
                     YLKKTPGGFAPALRYFTGQVISRVYNDREGNRWFTTIGNGVYKSPANNPQISAQYIPN
                     DEHREQVFSIIAARDGSQWIGTSNGSVYHREGGRLSRFDLLKENNTINRVLSLLEDST
                     GNILCAADRGLFLLARRSDGSYEEARPIPSIEGFEVAGLKRLFRDRNGTNYTSSLTGI
                     RPVIQRNGRLYQGELTHPDINFRVYAPFFASDGRLWFEHFERLSYLEGDKVREFPQFD
                     SLFISRITVINETDDGCLLIGTYGRGLYVMKNDAIIFHADYNSLGLTGDHCTKLVRRE
                     GKIFLATTNGVNILQWDGASLHLIEKFANRDGLLNEYTNDLAIDNRYLYLATPDGVCT
                     IDRIRKKTKALPPTTYITDFEILGQSVSLKAPIVVQPDSNSIGLTYIAP"
BASE COUNT          976 a         1129 c         1241 g         1008 t
ORIGIN      
        1 cagcggacag gccggtagcg attcgatcac ggttatcttc gatgtctgcg accaggtcgg
       61 aacgccgctt ggcgatcgta tcgcgctcta tccggtacct tccaccggtc gtatcaacat
      121 ttcggaaagc ccggtgccgt atcgactggt catcaccgat ctgacgggtc gcgccgtatg
      181 ggtagaatcg gtgcctgcag ggaatgtgaa gcagctgaaa ttgccttcag gagtattcca
      241 ttttctattg caaacagagc aacaggaaat cttactcagt cggaaggtgg ttgtcctgga
      301 atagtcgggg gtaatattcg cggcttagtg caagtcgaag ctgcggacca gatcacggtt
      361 gggtgatccg ctgtggcacc cgatgcaacc atctcccttt ttacctacac tgaaaacagt
      421 agatccgtcc ggttcatatt ctgcccacat ccagccgctg ccggcatttg cgttcgaggg
      481 ctccttcttc atgaccgcca ataaggttac gccggaaccc gacacgactt ccttaacgat
      541 caccgagccg ttcgggaaag tcgccccgga cgggagttcg cctgttgaat cgagcgcggc
      601 caatgcggtg gagttgaatt tcagtttgaa agagccgtgc gggctggggg acgcggcgct
      661 cagcgtgttt ccattttggt aatacgaata actaccgttc cggatctccg aagagaacgc
      721 ctgatcagca gatgattctt ctttgtcctt cttacacgcg atcatcgttg taactgccgg
      781 aagcagcatc aacattaact tgagagagtt gttcatggca gagggaatgg aaggggttaa
      841 caagagacag atccaatcgg aatcgaagtt tctatcaatc ccctaaagct aacgcaggca
      901 gctatccaat gattgtgatg attgtcacga atcacaggat gaaagcaaga aaaaggaaga
      961 agcgctattc gatgatcagt gtttttcgct caatacgggt tccttccgcg atgcgaacca
     1021 gatagcaccc actcgatagt cctgtgagat caaggcgcag tacaggtgca gagaggtcgt
     1081 gcatagaccg gatcaattgt ccttgcatgt tgtaaagctg gacaaggtcg ggtcgcgcat
     1141 ggtcgaatcg gaggttgacg aaatcgtgcg ccggattcgg aaagagtgtg aggcgggaag
     1201 gttcggctac cggcttgggg ccgagccaga ggttttcatc gaagcgcacc acgccgcttt
     1261 gttccgttcc ggcccagacg attccgtttc cttcaataca ggtataaaag acattgtcgt
     1321 ccggcatgtc ggagttcgcg gtgtaccagc tgtagtaaac cgggccttct tttttcacga
     1381 tgccacgatc gtaggtgccc aaccagatgt tggtcagtga atcgaactgc agagagttga
     1441 gcgaattggt ggggataccg gaactgagcg tattgtaagt gaaccaaccg ccgttgtaga
     1501 aattgaccag cgcgccggca ggacatgtca tccacaggtg gttaagcgga cccagtacaa
     1561 ggtcgttcat gctgttgtcg ggaaagttct ggtgcatgta gtcccaggtg gtgaaccccg
     1621 tatcacggat catttgggaa aatccgccgt tcaccgttcc cagccagcgc agcgtatcgt
     1681 tttcaatcac gcagcgtgag atgtgatcaa cgggaatgtt cgaattgcca atgttccagg
     1741 tcgtccagtt ggcgccgtcg aaccggctaa gtccggcgat ggtacagatc catgcgctgc
     1801 caaagcgatc gatcgtaacg cttcggatat agtcggcggc gatcggcgag ttggccgtgg
     1861 tgtagctggt ccagttcgta ccgtcgaata cgcagacgcc tccgttctgt gtcgagatcc
     1921 acgcccgacc agccgcatcg aacgcgacat gcttgacaga attgtcggtg agaccactgt
     1981 tcgtcgtctg gtaaaccgtc caggtgttgt cgtcgtaaat cgcaagaccg aattcggtac
     2041 agaaccattt ccggttcaga ctgtcgatcg cgatgtagcg gcattggttg tcgggaagcg
     2101 gggagttatt tcggtcgaag acagtaaagg tctgttgcgc ggaggcgaac agggaccaga
     2161 tcagcaggag tagggtcagg cgcatgtcga acaaatatac tgaagccctt gaagcggagg
     2221 atgacgcccg tcacgtaatc gctgaacttt cccgccttgc gcgcgaatcc gccacaggag
     2281 cggctttcgg ggactgttgc aagccccggg ggcattgatt aacttgattc cctacgtgag
     2341 acctttcctg cgccttttaa cctgcctgtt ttgcttcctg ttgtccggtg cccgggcaca
     2401 ggatcccgtt taccgggtct atgaccggcg cgacggactt ccttcgaata ccgtctactg
     2461 cgcatttcag gacgcccggg gttttttgtg gttcggcacg gatgcgggcg cttcccgctt
     2521 cgacgggcgg gcgtttcgtg gatacaatgt gcagaacgga ctggtcgaca gcgaagtcct
     2581 gcgcatctat caggattcga agggacgcat ctggtttctg accctgaaag gattgttgtg
     2641 ctattacctc gacggtaaga tccataatcc ctacaatgac cccgacctgc cgaagggtct
     2701 ggcggatcgt ggcttgttgt cgttccggga agacgcgaag ggaaatatct ggttcggagg
     2761 gctgagtaac caggtgctgc gcattgatcc gtcggatcac tgtgatttta tcatgcccga
     2821 tacgatcgcc aatcctgcgc gtgcgggctg gacctttctc tatgaacccg agcccggcgt
     2881 catgatcgtg aacgtgcagg atggattggt tcgcatcaac tcggacgatc tgaaggaaac
     2941 gccggttgaa atgccgggca tcggcgatac cgtggcctgg atggaaaata ccggtccgcg
     3001 aacgggattg ctgatcagta cgcgtgggct ctttcgcctc aatggctacg aggcggaatt
     3061 gctgctgagc gccgccgaga tccccgcgca gatgaacatg ggacgcatca cacaggatga
     3121 agacggcagt ctgtggatga tgaacaacga caaatgcgtg tactatctga agaagacacc
     3181 cggcggattc gctccggccc tccgatactt caccggccag gtgatcagcc gggtctacaa
     3241 cgatcgtgaa ggcaatcgtt ggttcacgac gatcggcaac ggggtctata aatcgccggc
     3301 caacaatcct cagatttcgg cccagtacat tcccaacgac gagcatcggg aacaggtgtt
     3361 ctccattatc gccgctcgcg acgggagcca gtggatcggt accagcaacg gatcggtgta
     3421 tcatcgggaa ggcggccgac tgtctcgctt tgacctgttg aaggaaaaca acaccatcaa
     3481 ccgggtcttg tctttattgg aagattccac cgggaatatc ttatgtgccg cagaccgagg
     3541 cttgttcctc ctggctcgac ggagtgatgg gtcatatgag gaggcccgtc ccattccatc
     3601 gattgagggt tttgaagtcg ccggcttgaa gcgattgttc cgtgaccgca acggcacgaa
     3661 ttacaccagt tcccttaccg gtatccgacc tgtcatccaa cggaatggtc gcctgtacca
     3721 gggggagctg acgcatccgg acatcaattt ccgggtctat gccccgttct ttgccagcga
     3781 cggacgattg tggttcgaac attttgagcg actatcctat ctggaaggtg acaaagtccg
     3841 ggagttcccg cagttcgatt ccttgttcat cagccgtatt acggtaatca acgagacgga
     3901 cgacggatgt ctgttgatcg gcacctatgg tcggggtttg tatgtgatga aaaatgatgc
     3961 gatcattttt cacgcggatt acaattccct cggtcttacc ggcgaccatt gcaccaagct
     4021 cgtccgccgg gaaggaaaga tcttcctcgc gactacgaac ggtgtgaata ttctgcagtg
     4081 ggatggagct tcccttcacc tgatcgaaaa attcgccaac cgcgacggtt tgctgaatga
     4141 gtacaccaac gatctggcta ttgacaaccg gtatctatac ttggctactc cggatggcgt
     4201 ttgcaccatc gacaggatcc ggaagaagac gaaagccctg ccgccgacga cttacatcac
     4261 cgattttgaa atcctcgggc aatcggtatc gttaaaagct ccgatcgttg ttcagcccga
     4321 cagcaattcg atcggactga cctatatcgc acca
//
LOCUS       DAOTON010000123         4325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16520_length_4325_cov_4.148009, whole genome shotgun sequence.
ACCESSION   DAOTON010000123 DAOTON010000000
VERSION     DAOTON010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16520_length_4325_cov_4.148009"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="P5292_03535"
     CDS             complement(<1..1402)
                     /locus_tag="P5292_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38229.1"
                     /translation="MKTLQNDRKDRSRTTMSGRMQQLAFLLLGLILFSGKSSQAGALH
                     DFTASDAAVNNMLNRFDEKIYFTRNQGQYRQGVLFKADFPLGQAIATPQGMLIKAYDP
                     ASVEARREDGERIEQEIHDGLPARALNVNLRGHGWMMRFLNSSASMKVEGRDAHGDPM
                     NFFMGNKSAVGVQSFQEVWYRNVYRNVDVRYYPAEDGTMEYDIICKPGFDASRIAVSF
                     DGLKELQVIDGRLVLATSVGDIAVPAPVAYQQINGQREFVSAAYTVQTNILRFQLGKY
                     DPAYPVIIDPIALRWATWVNTNSSSDNHGHCIWVDPTDGAIYMVARVVGTTDEITVGA
                     FDETANGNLDMIVGKYLEPSAVGQSGTRVWQTYLGGSGDDNPYAMEQGPDGNLYITGY
                     TSSSNYPLIGGGAFTGSSVDQRGQTVNNIFVTKITPDGASIKSAVIGGNQDEGSFDIR
                     LDATGNPVVCGNTRSTN"
     gene            complement(1944..3995)
                     /locus_tag="P5292_03540"
     CDS             complement(1944..3995)
                     /locus_tag="P5292_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38230.1"
                     /translation="MKNRYALRLIQVFLLLLATESSLAQWQPLNGPFSGYVSSIASDN
                     DYRYAGTRGGGFFRYTDVAGTWSRVNFDLLYYDYYDLLISNDTIFAATNNGVAYSTDQ
                     GVNWTLSNNGINDAVSEILLLNGIYYAASGYSGVFVSTDGGMNWNPSNNGLTTLTLYD
                     IEHDGNDLYVATDGEGVFKSTDGGANWIAVNNGITGTIAQAISAGGGYIFAAISSDGV
                     YRSSDQGANWEPANTGMPTVYYFPAMEYYGSELYVGTYGYGFWKTSDNGDNWTEINTG
                     ILNPQGYSVYSENGNLWAGSGAGIFKSTNSGTSWTDMSDGMLAAQVTRIVQTGTSLIC
                     ILEDGGLVRSSDGGANWSSSNTGFTEVNQQDLYVYGSKIFCGTNYSSVYVSTDDGASW
                     SPSGNGITNPNIGCFTSYGNKLFAGSIGGGVYFSDDDGANWSEVNNGLTTQVISSLAN
                     TGTEVFAATYSAGVFRTTDDGANWNVVNNGLTNLSVFSLVSLNGNLYAGTNGDGVFMS
                     DDGGANWSQINNGLGSLYIRRLYSDGTYLYSCSDFAGIFISGDGGLTWEEFNNGLVNT
                     NLYDVVSDGVNLIAATAGASTWMRPVSDFTGLGSMNDSKSSVRLFPNPSSGRFTIKGL
                     PAEGSSTITLYDLTGREFRRVVTQGEQQCSFDVEDLPKGSYIVSILSDRLVAISVLVL
                     N"
     gene            complement(4130..>4325)
                     /locus_tag="P5292_03545"
     CDS             complement(4130..>4325)
                     /locus_tag="P5292_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38231.1"
                     /translation="STAIGSEKDQEVAVCYFFSKAGNQSGKQALATSGRDFQNIEAEV
                     LGAIGLDKLLLGNTFATLHD"
BASE COUNT         1030 a         1085 c         1166 g         1044 t
ORIGIN      
        1 gattcgtact gcgcgtgttg ccgcaaacca ccgggttgcc ggttgcatca agacgaatgt
       61 cgaaggaacc ttcatcctgg ttgccaccga tcacggcgct tttgatagaa gctccatctg
      121 gcgtgatctt ggtgacgaat atgttgttga cggtttgacc gcgctggtcg acgctgctac
      181 ccgtaaacgc gccaccgccg atcagcggat aattcgagct ggaggtgtaa ccggtaatgt
      241 atagattgcc atccggaccc tgttccatcg cataaggatt gtcatcgccg ctgccgccaa
      301 gatacgtctg ccatacacgc gtaccagatt ggcctaccgc ggagggttcc agatattttc
      361 cgacgatcat atcaagatta ccattggccg tttcatcgaa tgcccctact gtgatctcgt
      421 cagtagtgcc aacaactcgg gctaccatgt agatggctcc atcggtagga tctacccaga
      481 tgcagtggcc gtggttgtcg ctactcgagt tcgtattcac ccaggtcgcc caacggagcg
      541 cgatcggatc gataatgacc ggataagccg gatcgtattt accaagctgg aatcggagaa
      601 tgttggtttg aaccgtatag gctgcgctga cgaattcccg ctgaccgttg atctgctgat
      661 acgcaaccgg agcgggaacc gcgatatcac cgacagatgt cgcgagcaca agtcgtccgt
      721 ctatcacctg tagttccttt aagccgtcga acgataccgc aatgcgggaa gcatcaaacc
      781 ccggtttgca gatgatgtca tattccattg tgccgtcttc ggcagggtag tagcggacat
      841 ccacgttgcg gtagacattc cggtaccaca cttcctgaaa actctgaacg cccactgcag
      901 acttgtttcc catgaagaaa ttcatcgggt caccgtgtgc gtctcgtcct tccactttca
      961 tcgaagccga actgttgagg aatcgcatca tccagccgtg tccgcgcaga ttgacgttca
     1021 gcgcacgtgc cggaagcccg tcatggatct cttgttcaat gcgttcacca tcttcacgtc
     1081 gagcttcaac agaagcggga tcgtaggctt tgatcagcat cccctgcggg gtagcgatcg
     1141 cctggccgag cgggaaatcc gccttgaaaa ggacgccctg ccggtactgc ccctggttgc
     1201 gggtgaaata gattttctcg tcgaagcggt tcagcatatt gttgaccgca gcgtccgatg
     1261 ctgtaaaatc atgcagcgcg ccggcttgcg atgattttcc gctaaaaagg atcagtccca
     1321 acaggaggaa ggccagttgt tgcatccttc cggacatggt tgtccgagat cgatcttttc
     1381 gatcgttttg aagtgttttc attgcagaag tgtagttgtg tgaatgcatc gaatgaagct
     1441 gatttaacgt gggcaaagta gcttcccgat gggccgaagc tcaattatat tcggcgatca
     1501 tgcattccgg atgtgtcaat gcagcaatct gtattgtgaa aaggcggact aatgaatttg
     1561 tatgatgatg gggattttat tgaaatggac ccaattcacc gcctataata tgtcgaagaa
     1621 ctgcccggaa taatcttcaa tctgcaatta atccttacac cagccttttt cgctgacact
     1681 tcacccggat aatgggagtt gatttattgc aatgctacca aggctgaata cctgaggctt
     1741 ttctaactac gaggaatcag cgaccgttta tgcattgctt ccgagtagta atcggctgat
     1801 gcacatggat gccagggttg attcacacca cgttgaattt gcgaaaacgt taaccgtata
     1861 cgattaagag aatttagttt cgaccgatat aaaaagaaga gcggtcgtta ttcacgaccg
     1921 cccctcatac attttcctac agatcaattc aagaccagca cagaaatagc aacaagccgg
     1981 tcgctaagga tactcacgat gtagctccct tttggcagat cctcgacgtc gaacgaacac
     2041 tgctgttcgc cttgtgtgac gacacgccgg aattcgcggc cggtgagatc gtatagcgtg
     2101 atggtggaag atccctcagc gggcaagcct ttgatggtaa accggccgct ggaaggattg
     2161 ggaaacagcc gaactgacga tttggaatcg ttcatggaac cgagccccgt aaaatcgctg
     2221 accggccgca tccaggtcga agcgcctgca gtggcggcaa tcaggttgac gccgtcgctg
     2281 accacatcat aaaggttggt gttgacgagc ccgttgttga attcctccca ggttagaccg
     2341 ccgtcgccgg agatgaagat accggcgaaa tcggagcagg aatacaggta ggtgccatcc
     2401 gaatagagtc gtcggatata caaagaaccc aatccgttgt taatctgtga ccagttggcg
     2461 ccgccgtcat ccgacatgaa gacgccatcg ccgttggttc cggcgtagag gttgccgttc
     2521 agggacacca ggctgaatac cgaaaggttg gtcagaccat tgttaacgac attccagttt
     2581 gctccgtcat ccgtagtacg aaagacaccg gcagaatacg tggctgcgaa aacctctgtt
     2641 cccgtattgg ccagcgagga gatgacctgg gtggtgaggc cattgttcac ttctgaccaa
     2701 ttcgcgccgt cgtcgtccga gaaatagact cctccgccga tggatccggc aaagagtttg
     2761 tttccatacg aagtgaaaca tccgatattg ggattggtga tgccgttgcc ggatggcgac
     2821 cagctcgcgc catcgtccgt agaaacatag acggatgaat agttggttcc gcaaaagatc
     2881 ttgctgccgt acacatagag atcctgttgg ttcacttccg tgaaaccggt attgctgctc
     2941 gaccagttag ctccgccatc gctggaacgg accaatccgc cgtcttcgag aatgcagatc
     3001 aatgaggttc cggtttggac aattcgtgta acctgcgccg ccagcatacc gtctgacata
     3061 tcggtccaac tggttccact gttggtggac ttgaagatgc ctgcaccgga accggcccag
     3121 agatttccat tctctgaata cacggaatat ccctgcggat tgaggatgcc ggtgttaatc
     3181 tccgtccagt tgtcgccgtt gtcggaagtt ttccagaaac catagccgta ggtgccgacg
     3241 taaagttccg agccgtagta ttccatggcg gggaaataat aaactgtcgg catgccggtg
     3301 ttggcgggtt cccagttcgc gccctgatcg gaggagcgat aaactccgtc ggatgaaatg
     3361 gccgcgaaga tgtatcctcc gcccgcggaa attgcctgcg cgatcgtgcc ggtgatgccg
     3421 ttgtttactg caatccagtt cgcgccgcca tctgttgact taaacactcc ctctccgtcg
     3481 gtggcaacat acagatcgtt gccgtcatgc tcaatgtcat agagcgtaag tgtagtgagt
     3541 ccgttgttgg agggattcca gttcataccg ccgtccgttg aaacgaaaac gccgctataa
     3601 ccgctagcgg catagtaaat gccgtttagt aagaggattt cggaaacggc atcgttgatc
     3661 ccgttgttgg aaagggtcca gtttacgcct tgatccgtag aataagcaac tccattattg
     3721 gttgcagcga aaatggtatc gttgctgatg aggaggtcat agtaatcata ataaagcaga
     3781 tcgaaattga ctcgggacca ggttccggcg acatcggtat agcggaagaa tccgccgccg
     3841 cgggtaccgg catatcggta atcgttgtcc gaggcgatgg acgagacata tcccgagaag
     3901 gggccattca acggttgcca ttgggccagc gaagattccg ttgccaacag caataggaag
     3961 acctggatga gccgaagcgc gtagcgattt ttcattggat cgaagtgtag tggatgaagt
     4021 gacaagatcg atagaaggga ttgtttgtgc aaacgagtga ttaattcagc aaaaaccccc
     4081 tccggattca ccggaagggg gcaggtgtgt gtcatttcag aaaagctcgt cagtcatgga
     4141 gagttgcaaa tgtgttgccg aggaggagct tgtcgagtcc gatagcgccc aaaacttctg
     4201 cttcgatgtt ttggaagtca cgaccggaag tagcgagtgc ttgcttgccg gattggttgc
     4261 cggctttact gaagaagtag caaactgcaa cttcctggtc cttttcgctt ccgatagcgg
     4321 tcgag
//
LOCUS       DAOTON010000124         4320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16547_length_4320_cov_3.156858, whole genome shotgun sequence.
ACCESSION   DAOTON010000124 DAOTON010000000
VERSION     DAOTON010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16547_length_4320_cov_3.156858"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="P5292_03550"
     CDS             complement(<1..390)
                     /locus_tag="P5292_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38232.1"
                     /translation="MKRLLLAACALLATLGATAQGIWVSQATNFTPVSSGVRYISAVD
                     TNVVWICSYDGSGGGASRQDFSRTVDGGATWTAGTVPCPTSHDWSMINAVSADSAWAV
                     FYNATAGSGGGIWRTADGGANWTQVGVG"
     gene            complement(576..1178)
                     /locus_tag="P5292_03555"
     CDS             complement(576..1178)
                     /locus_tag="P5292_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRS38233.1"
                     /translation="MKQLLTTLVLVFVVLAAQAGYKVGDEARDFALKNVDGTSVSLSK
                     FAGDTKGAIVIFTCNHCPFSVAYEDRILALDKKYAALGYPVIAINPNDAKIQPGDSYE
                     NMIERAKEKNFTFPYLHDETQEIATAFGAARTPHVYVLEREKDKFIVRYIGAIDNNSD
                     EPGAVTSKFVEEAVDALVAGKPVTTAETKAIGCTIKWTQK"
     gene            complement(1218..1709)
                     /locus_tag="P5292_03560"
     CDS             complement(1218..1709)
                     /locus_tag="P5292_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRS38234.1"
                     /translation="MRFPLLLVVSLYFQVPGSVKAQVIHRMSFDGMEQRLHGISDSLV
                     VLNFWATWCKPCVEELPYFDSAPDRHPDRPLKIILVNLDFNSRVSSLAEPFVVKRKVR
                     SEVWHLDDTDPNTWINRLDPAWSGAIPASAFYYNGKKLDFREGQLTQVELDQIIQRLS
                     EHR"
     gene            1782..1877
                     /locus_tag="P5292_03565"
     CDS             1782..1877
                     /locus_tag="P5292_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HRS38235.1"
                     /translation="MGRGDKRSRKGKIWRGTSGKSRPARKTNKRI"
     gene            complement(1949..3430)
                     /locus_tag="P5292_03570"
     CDS             complement(1949..3430)
                     /locus_tag="P5292_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRS38236.1"
                     /translation="MSYSLEQIRDFKGKYPKQLWYLFFSEMWERFCFYGMRGMLTVFM
                     VDKIVGLSMREDVANLQYGATQAFVYAFTFIGGLFADKILGFRKSLFWGGLLMISGSC
                     ILAADPKDFFFLGISFTIVGTGFFKPNISTMVGALYNDGDHRRDAGFSLFYAGINIGA
                     LLGGYACVAIAKSYSWNLAFGLAAIVMTISLLTFVFTQRSLGPIGRSPMEAGKGGKRW
                     YEYATYIGSLAVIPLIMTMVAKTEYTDWFMYIIGPCTLIYMFYEMTRYSAEERKKLIA
                     ALVFILFSVLFWAFFEQSGGSLNLFASNNLKDSFLGVKLDPNGVNNSANSLFVIIFAP
                     LSGLFWLWLSNRKAEPNTVVKFGLGFLFLSLAFYVFYSTRFFAGADGMTSLEVFTLAY
                     FVITFGELCLSPIGLSIMTKLSPQPLQGVMMGMWFLASAYGQYVAGILGAGMASPNEN
                     ATLSEKLISYTDGYQQLAIYAVISGVVLIAISPMVRRLMGNVK"
     gene            complement(3558..>4320)
                     /locus_tag="P5292_03575"
     CDS             complement(3558..>4320)
                     /locus_tag="P5292_03575"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRS38237.1"
                     /translation="EGLKLDLVRILSIECPVSEQEFLNGKSEAITEKVFAEAQRYYRH
                     KSQLMIDRTWPFINDVFTNQSATIQNIVVPFSDGMKGIQVIANLRKSHDSHGRELISA
                     FERTIISALIDDAWKEHLREMDDLKTSVQNAVYEQKDPLLIYKFESFELFKRMVTETN
                     RDIISFLFKAVIPQQDPNQVREAKPQPRQDLRNVQTSKADASEAQRSNGIPQGPPPSA
                     PKAQPVRVEQKVGRNDPCPCGSGKKFKNCHGAGTL"
BASE COUNT         1010 a         1099 c         1202 g         1009 t
ORIGIN      
        1 acctacacct acttgggtcc agttggcacc gccatcagcc gtgcgccaaa tgccaccgcc
       61 ggaaccggca gtcgcattgt agaatacggc ccatgcagaa tccgcagata ctgcattgat
      121 catcgaccag tcgtgcgagg tcgggcaggg aacagttccc gccgtccagg tggctccacc
      181 gtcaacggta cgggagaaat cctgacggct ggcgccaccg ccagagccgt cgtaggaaca
      241 gatccataca acgttggtgt caacggcgct gatgtagcgt acaccggaag ataccggcgt
      301 gaaattcgtg gcctgactga cccagattcc ctgggcagta gcgcccagcg ttgcaagaag
      361 ggcacaggct gcaagtaaca gtcgtttcat tttgttggta attggttttt ggttagtcgg
      421 ttgctttaaa agaggagccc caagttacga tttcccggcc tgtcgcacta tcaaaaccgg
      481 taagccaagt gtcataaaac acgttgaaaa acaggcagat gaagaaaatg cctcccggat
      541 ggggaggcat taacattttc aagcgttatg gaactttatt tctgggtcca cttgatggta
      601 caaccgatgg ctttggtttc cgcggtggtg accggttttc ccgccaccaa tgcatcgacg
      661 gcttcctcaa cgaatttgga agtaacggct cccggttcgt cactgttgtt gtcgatcgcg
      721 ccgatatacc gtacaatgaa tttgtctttt tcccgctcaa ggacatatac atgaggcgtg
      781 cgtgctgcgc cgaatgccgt agcgatctcc tgcgtttcgt cgtgcaggta aggaaatgtg
      841 aagttctttt ctttcgcacg ttcgatcata ttctcgtaag aatcacccgg ctggatcttg
      901 gcatcgttcg ggttgatggc aatcaccgga tatccaagtg cagcatactt cttgtccaag
      961 gccaggatgc ggtcttcgta cgcgacggag aagggacaat ggttgcaggt gaaaatgacg
     1021 atggcgcctt tggtatcacc ggcgaatttg gagagcgaga cggatgtgcc gtcaacattt
     1081 ttcagcgcga agtcacgcgc ttcgtcaccg actttatacc cggcttgcgc ggcaagtacg
     1141 acgaatacca acacgagggt agtgaggagt tgtttcatgg ttgttatggt ttgagttatg
     1201 atgctggttc tggatgatta tcggtgttcg gaaaggcgtt ggatgatttg atccagttca
     1261 acctgtgtca attgcccttc gcgaaagtcg agtttttttc cattgtaata aaaggcggat
     1321 gccggaatgg cgccactcca tgccggatca aggcggttga tccaggtatt ggggtcggtg
     1381 tcatccaggt gccaaacctc ggatcgtact tttcttttga cgacaaaggg ttccgcaagc
     1441 gaactgaccc gcgagttaaa atccagattg actaagatga tcttgagggg tcgatccggg
     1501 tggcgatcag gagctgagtc gaagtacggc aattcctcca cgcagggttt gcaccaggtc
     1561 gcccagaaat tcaagaccac cagactgtcc gaaataccat gcaatcgctg ttccatcccg
     1621 tcaaaggaca tccggtggat cacctgggcc ttgaccgaac ccggtacttg aaagtatagg
     1681 ctaaccacaa gtaagagcgg gaatcgcatg ctataaaaat aacattctgg ttgctggatt
     1741 gttccggcct ttctattttt aagccgttaa aaaactgaat catgggaaga ggtgacaaac
     1801 gttcccgtaa agggaaaatc tggcgcggca ccagcggcaa atcccgcccg gcgcgcaaga
     1861 ccaacaaacg gatctgatac aatcggttcg tcgatcttta cagcatgcgg tactgccgca
     1921 tgctgttttc gtttgtacgc ccggctgttt atttcacgtt gcccatcaga cgacggacca
     1981 tcggagaaat ggcgatcagg accacgccgc tgatgacggc atagatggcc aactgctggt
     2041 agccgtcggt ataggagatc aatttttcgg ataaggtcgc gttttcattg ggcgatgcca
     2101 tacctgcccc gaggatgcct gctacgtatt gcccgtaggc gctggcgaga aaccacatgc
     2161 ccatcatcac gccctgtaag ggctgtgggg aaagtttcgt catgatcgat agtccgatcg
     2221 gcgagaggca aagctcgcca aaggtgatca cgaaataggc cagggtaaat acttccagcg
     2281 aggtcatgcc atcggcgccc gcgaagaatc gggtggagta gaagacgtaa aacgcaagcg
     2341 acaaaaaaag gaatccgaga ccgaatttca ccaccgtgtt cggttccgct ttccggttgc
     2401 tcagccataa ccagaagagc ccggacaagg gcgcgaagat gatcacgaag agtgaattgg
     2461 cggaattgtt gacgccgttc gggtcaagct ttactcccag gaaggaatcc ttgagattgt
     2521 tgctggcgaa caggttcagg gagccaccgc tttgttcgaa gaaagcccag aagagtaccg
     2581 aaaagagaat gaagaccagt gcagcgatca gcttcttacg ttcctcggcg ctgtaccgcg
     2641 tcatctcgta gaacatgtag atcagtgtgc agggcccgat gatgtacatg aaccagtcgg
     2701 tatactccgt cttggccacc atggtcatga tcagcggaat gaccgccagc gaaccgatgt
     2761 aggtcgcgta ctcgtaccac cgctttccac ccttgccggc ttccatcggc gaacgaccga
     2821 tcggaccgag gctgcgttgg gtgaaaacga aggtcagcag actgatggtc atcacaatgg
     2881 cggccagacc gaatgcaagg ttccacgaat aactttttgc gatcgctaca caggcatatc
     2941 cgcccagcaa ggcgccgatg ttgatgcctg cgtagaagag cgaaaagccc gcgtcccgcc
     3001 gatggtcccc gtcgttgtac aacgccccga ccatggtcga tatgttaggc ttgaagaatc
     3061 cggtaccgac aattgtgaaa ctgatcccca ggaaaaagaa atccttcgga tcggctgcaa
     3121 ggatgcaact acctgaaatc atcaggagcc cgccccagaa gagcgatttc cggaatccga
     3181 ggatcttgtc tgcgaacagt cccccgataa aagtgaaagc gtacacgaac gcttgcgttg
     3241 caccgtactg caaattggcg acgtcttccc gcatgctgag accgacgatc ttgtcgacca
     3301 tgaagacggt caacatccca cgcattccgt agaagcaaaa acgctcccac atttcggaaa
     3361 agaaaagata ccagagttgc ttcgggtact ttcctttgaa gtcccggatc tgttctaagc
     3421 tgtagctcat agcgtggtgg gggcgttagc gcgtgcccga caagtataag caattcaatc
     3481 ttttggttcc attgtaaaaa ggtcaaaaaa aagccccgga ggaagttcct tcggggctgt
     3541 agtgtttatt atccggctca aagcgtacct gcgccgtggc agtttttgaa ttttttgccg
     3601 ctgccacaag ggcatggatc gtttcgtcct actttttgtt cgacgcgaac gggttgggct
     3661 ttcggcgcgg acggcggcgg tccttgggga atgccgttgg aacgctgtgc ttcactggca
     3721 tcggccttgc tggtctgtac gttgcgcagg tcctgacggg gttgaggctt cgcttcccgc
     3781 acctggttcg ggtcctgttg cggaatgacg gctttgaaaa ggaaggagat gatgtcccgg
     3841 ttcgtttccg tcaccatgcg cttgaacaat tcgaacgact cgaacttgta aatgagcagc
     3901 gggtccttct gttcgtacac cgcgttctgc acggacgtct tcaggtcgtc catctcccgc
     3961 aaatgctctt tccaggcgtc gtcgatgagc gctgaaatga tggtgcgttc aaatgccgaa
     4021 atgagttccc gcccgtgcga gtcgtgcgac ttccgcaggt tggcgatcac ctggatgcct
     4081 ttcatgccgt ccgagaacgg cacgacgata ttctggatgg tcgcgctttg gttcgtgaaa
     4141 acgtcgttga tgaacggcca ggtgcggtcg atcatcaatt gggatttgtg acggtagtat
     4201 cgctgcgctt cggcgaatac tttctccgtg atggcttcgg attttccgtt gaggaattcc
     4261 tgttcgctga cggggcactc gatcgaaagg atccggacaa ggtcgagttt cagaccctcg
//
LOCUS       DAOTON010000125         4314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16577_length_4314_cov_3.001174, whole genome shotgun sequence.
ACCESSION   DAOTON010000125 DAOTON010000000
VERSION     DAOTON010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16577_length_4314_cov_3.001174"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="P5292_03580"
     CDS             <1..131
                     /locus_tag="P5292_03580"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HRS38238.1"
                     /translation="FIKLLGANVNLGNIAPNEVIAVESLRIGLRSDTFFQYLDKKH"
     gene            142..630
                     /locus_tag="P5292_03585"
     CDS             142..630
                     /locus_tag="P5292_03585"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRS38239.1"
                     /translation="MPRFRILSIPCLVACSFLHTGCLTMKSVKISSIDALQVELNGNV
                     PNIRFDLRVENPNNWGVRLLEAETGLLIDNKPVGTAKADQRLKIRAKSETAVPVSIQT
                     SYMELLKMAPQGLELLSGQRSVEGRAVGSLKIGKFIFRKRIAFDFSEKLDQQRLKKLF
                     GK"
     gene            636..1208
                     /locus_tag="P5292_03590"
     CDS             636..1208
                     /locus_tag="P5292_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRS38240.1"
                     /translation="MNQVMSHPEIRTAIDRLRVCLARVDKLKDLRPDQLLWKPAPHRW
                     SIAQCLDHLIVSNECYFPVLDRLCGDRYQATFWERWSPFSKSIGRNMVATLGPEVRRP
                     FKAPKIFQPRQKEVKDLAIVDRFRKHQETLIGKLEAIRVQTINKVVVTSPVAGLITIP
                     FPDVLEILAGHEERHLSQAERLLVETGFPV"
     gene            complement(1284..2264)
                     /locus_tag="P5292_03595"
     CDS             complement(1284..2264)
                     /locus_tag="P5292_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38241.1"
                     /translation="MKQRIAPLEAISWTVLLLAALLFGTAIAYAGEPKKDQRKKKEDK
                     HEGKAVISICKEDNGKVIRLDTTINLADEAAIEAALKKLELGDDFHFDLRMPGEHGKE
                     DAFTMKFWSEDLSPDDRKRMKDELAAAMRDSERSLEDAHKAMKEFHMEIKGMDEEGSG
                     ALSMHFPADFDFEFPEHFRCGVGDPELDSLQSDDRILIIGKPDEAAPVFEKTVTTASG
                     KQVFVFQRQLSQEAQRSRAAEKQLDIRDIEIFPNPADDRLTLSFEKEKTGDITIRLMD
                     LQGKVVYDETLRAFSGAYYKRIDISGIAAGTYTLSMEQGESRITRKVIIR"
     gene            2393..4060
                     /locus_tag="P5292_03600"
     CDS             2393..4060
                     /locus_tag="P5292_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRS38242.1"
                     /translation="MNKRQIRWIIALMSSALIGIIGLQVYWILHDIHLKEQQFDQTVN
                     QAMNAIVDRIERSEAADVLQRRFFDIDPATISHMMMSEDDADEEDLVVPSAQKVEMVT
                     DTAFEVTDLHALQHSPYFFHDLDNADINIEFHRPGTNRTFLRIQRRNLFQRDSVSQHT
                     IRSSQITRVYGDSAEVIIRQNEEKIKARLEKLRDVMQKMAVEFAGKEPDIRQRIDPKL
                     LDSLISHELGNRGIHVPFQFGVLNGVKDSLVISKTDRHAGDLMNSKYRVLLFPNDIVS
                     KPDYLILQFPNSIRYVLSSMWLMLGSSTLFTFIIVFGFAFTIHVILRQKKLSDIKTDF
                     INNMTHEFKTPIATISLAVDSIKDPRVSSQPEKLEYFSRIIREENKRMNKQVEHVLQM
                     AQLEKGELSLRRETVDLHDTIRQAVDSIGMQVASRGGKIETRMEALHPAVTGDPVHLM
                     NVVLNLLDNANKYSPEAPDILIASNDHPGGILISVRDQGMGMTKETQKRIFEKFYRVP
                     TGNIHDIKGFGLGLSYVKAIVEKHRGWIRLDSDPGKGSTFEIYIPHS"
     gene            4076..>4314
                     /locus_tag="P5292_03605"
     CDS             4076..>4314
                     /locus_tag="P5292_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS38243.1"
                     /translation="MSISKPHILLVEDDPNFGSVLKNYLELNDYQVNLCQDGFAGYDQ
                     FVTGKYDICILDVMMPRKDGFTLAREIREKNPQVP"
BASE COUNT         1046 a         1151 c         1104 g         1013 t
ORIGIN      
        1 acttcatcaa actgctgggc gcgaacgtca accttggcaa catcgcgccc aatgaagtga
       61 tcgcggtcga atcgttacgc atcggacttc gcagcgatac ttttttccag tatctcgaca
      121 aaaagcactg aggccattgc catgccccgc tttcggatcc tgagcattcc ctgcctggtc
      181 gcttgttcgt ttcttcatac cggttgcctg accatgaaat cggtcaagat cagttccatc
      241 gatgccttgc aggtggagct gaatggaaac gtaccgaaca ttcgttttga cctgcgcgtg
      301 gaaaatccca acaactgggg tgtccgcttg ctggaggcgg aaaccgggtt gttgatcgac
      361 aacaaaccgg tggggacggc taaagctgat caacgattga aaatccgggc caaaagcgag
      421 acagccgttc cggtgtccat acagacttct tacatggaat tattgaaaat ggcaccgcaa
      481 ggattggaac ttttatcggg ccaacgttcg gtggaaggcc gcgcggtcgg tagtttgaag
      541 atcggcaagt tcatcttccg gaaacggatc gcgttcgact tcagcgaaaa gttggaccaa
      601 cagcgcctga aaaaactatt cgggaagtaa tcaggatgaa ccaggtaatg tcacatcccg
      661 agatccgaac ggccatcgat cgcctgcggg tatgccttgc tcgcgtggac aagctcaagg
      721 accttcgccc cgatcaattg ttgtggaagc ctgctccgca tcgctggagt atcgctcagt
      781 gcctggatca cctcattgtt tccaacgagt gctatttccc cgtactggac cggctctgcg
      841 gcgaccgata ccaggcgacc ttctgggaac gctggagtcc gttcagcaag agcatcggcc
      901 ggaatatggt tgccacactt ggcccggagg tgcgaaggcc tttcaaggca ccgaagatct
      961 ttcagccgcg acagaaagaa gtgaaggacc ttgcgatcgt cgatcgcttt cgaaagcatc
     1021 aggaaacgct gatcggcaaa ctggaagcta tccgggtcca gacgataaac aaagtcgtgg
     1081 tcacatcgcc cgttgccggc ctgattacca ttccattccc ggatgtgctt gagatccttg
     1141 ccgggcatga agagcgacat ttgtctcagg ctgagcgcct gctggtcgaa accggcttcc
     1201 cggtataatt aaaaaacccc ggatcatcgt ccggggtcaa cctgggaacc atcacgaaac
     1261 caggtacaat tgaaattgaa cgtttagcgt atgatcactt ttcgggtgat gcgcgattct
     1321 ccctgctcca tgctgagcgt ataggtacct gcggcaattc cggaaatatc gatgcgtttg
     1381 tagtatgcac ccgagaaagc acgaagggtt tcatcgtaca ccaccttccc ctgcaggtcc
     1441 atcagacgga tcgtgatatc gccggtcttt tccttttcaa agctgagggt gagccggtca
     1501 tcggccggat taggaaaaat ctcgatatcg cgaatgtcca actgcttctc ggcagcccgg
     1561 gagcgctgtg cttcctggct gagttgacgc tggaagacga acacttgttt tccggaagcg
     1621 gtggtaacgg tcttctcgaa aaccggtgca gcctcgtccg gttttccgat gatcaggata
     1681 cgatcgtccg actgaaggct gtccagttca ggatccccta caccgcaacg aaaatgctca
     1741 gggaattcga aatcaaagtc agccgggaaa tgcatggata aggcacctga gccttcttcg
     1801 tccatgccct tgatctccat atggaattcc ttcatggctt tatgggcatc ttccaaagac
     1861 cgttcactat cccgcatagc ggctgctagc tcgtccttca tacgtttccg gtcatccggg
     1921 ctaagatctt cactccagaa tttcattgtg aaggcatcct ccttaccatg ctcaccaggc
     1981 attcgcaggt caaaatggaa atcgtcgccg agctccagtt tcttcaaggc cgcttcgatg
     2041 gcggcttcat cggccaggtt gatggtggtg tcgagtcgaa tgactttccc gttgtcctct
     2101 ttgcaaatgg agatcacagc tttcccttcg tgcttgtctt ccttcttttt cctttgatcc
     2161 tttttaggct caccggcata agcgatggct gttccaaaga gcagtgctgc cagcagcaga
     2221 acggtccagg agatggcttc caagggggcg atgcgttgtt tcatggtgtt gtcgcttggt
     2281 tgacacaaag aaagcccggg aagcagccct ttgcggttaa tgagccgtta atctctgtta
     2341 aggtccggtt aatcatcccg ccaaatgtta aaggagggtt acttttgttg aaatgaataa
     2401 acgccaaatc cgctggatca tcgccctgat gagctcggcc ctgatcggga tcatcgggct
     2461 gcaggtttac tggattttgc acgatatcca cctgaaggag cagcaattcg accagacggt
     2521 caaccaggcc atgaatgcca ttgtcgaccg catagaacgg agcgaggctg ccgacgtgct
     2581 gcaaagaaga tttttcgata tcgatccggc gaccatcagc cacatgatga tgagcgaaga
     2641 cgatgccgat gaggaagacc tggtggtgcc ttctgctcag aaagtcgaaa tggtcacgga
     2701 taccgccttc gaggtaacgg acctgcatgc actccagcat tccccctatt ttttccatga
     2761 cctggacaat gcggatatca acatcgagtt tcaccgtccg ggtacgaacc gtacgttcct
     2821 tcggatccag cggcggaatc tcttccagcg cgattcggtt tcacagcata cgattcgttc
     2881 ttcccagatc acccgtgttt atggggattc cgccgaggtg atcatccggc agaacgaaga
     2941 gaaaataaaa gcccgactcg agaagctccg tgacgtgatg cagaaaatgg ccgttgagtt
     3001 cgccggaaag gaaccggata tccgacagcg gattgaccca aagctgctcg actccctgat
     3061 ctcccacgaa cttggcaacc gtggcatcca cgtcccattt caatttggcg tattgaacgg
     3121 cgtaaaggat tcactggtca tctcgaagac cgaccggcat gccggagacc tgatgaactc
     3181 caagtaccgg gtcttgttgt tcccgaacga catcgtatcc aaaccggatt atctgatcct
     3241 gcagtttccg aacagcatcc ggtatgtgct ttcttcgatg tggctgatgc tgggcagttc
     3301 aaccctgttc acgttcatca tcgtgtttgg attcgcgttt accatccatg tgatcctgcg
     3361 tcaaaagaag ttgtcggaca tcaagaccga tttcatcaac aacatgacgc acgaattcaa
     3421 aaccccgatc gcgacaattt cactggccgt cgactcgatc aaggatccgc gcgtatcctc
     3481 ccaaccggag aagctggaat atttctcccg catcatccgg gaagaaaaca aaaggatgaa
     3541 caagcaggtg gaacacgtac tgcagatggc gcagttggaa aagggagagc ttagccttcg
     3601 tcgcgagacg gtcgacctgc acgacaccat ccgtcaagcc gttgattcca tcggcatgca
     3661 ggttgcatcc cggggtggta agatcgaaac acgaatggaa gcgcttcacc ctgccgttac
     3721 cggtgatccg gttcatttga tgaacgtggt cctcaactta ctggacaatg ccaataagta
     3781 cagcccggaa gctccggata ttctgatcgc ttccaacgac catcccggag gcatattgat
     3841 ttccgtacgc gaccagggca tgggtatgac caaggaaacg caaaagcgaa tcttcgaaaa
     3901 gttctaccgg gtaccgaccg gcaacattca cgatatcaaa ggcttcggtt tgggtctgag
     3961 ttatgtcaaa gcaatcgtgg aaaagcaccg gggatggatc cgtcttgaca gcgatcccgg
     4021 gaaaggcagt acctttgaaa tttacatccc tcattcctaa taccctctct gcaacatgtc
     4081 catttcaaaa ccacacatcc tgttggtgga agatgatccc aacttcggtt cggttctcaa
     4141 aaactatctg gaattaaacg actaccaggt caacctgtgt caggacggat ttgccggcta
     4201 tgaccagttt gtcacgggca agtatgacat ctgcatcctc gatgtcatga tgcctagaaa
     4261 agacgggttc acccttgccc gggaaatccg ggaaaagaat ccacaagtac cgat
//
LOCUS       DAOTON010000126         4311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16593_length_4311_cov_3.423402, whole genome shotgun sequence.
ACCESSION   DAOTON010000126 DAOTON010000000
VERSION     DAOTON010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16593_length_4311_cov_3.423402"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /gene="carA"
                     /locus_tag="P5292_03610"
     CDS             <1..1034
                     /gene="carA"
                     /locus_tag="P5292_03610"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRS38244.1"
                     /translation="TTTGEICFNTGMTGYQEIFTDPSYFRQVLVATHVHIGNYGIHAD
                     EVESDGIKISGLVCRGFNVTPSRKSASESIQQYFDRQSVVAICDIDTRAVVRHIRNKG
                     AMNCIISSSVLDLDALKEQLSGVPSMAGLELSSLVTTKKPYTLGNPSARRRIAVMDYG
                     VKKNILRNLVERDSFLQVFPADTSFREIRSWNPDGILLSNGPGDPSVMPKQVAEIKLM
                     LEDNAPIFGICLGHQLLAEACGISTFKMFNGHRGINHPVKNLITGRSEITSQNHGFSV
                     NAEDVKAAKHLDITHVNLNDGTIEGIRVKDRVASSVQYHPEAAPGPHDSSYLFDDFIQ
                     CIDKAVVES"
     gene            1004..1681
                     /locus_tag="P5292_03615"
     CDS             1004..1681
                     /locus_tag="P5292_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HRS38245.1"
                     /translation="MHRQGRGRVLIPGNSGILPAIFFSLVLALFTSCRKEPATDQLAE
                     TFTVLTNRDPGADFSSYSTFFLSDTITYINDNPYDSILTGPGAGNLASSVRNELISRG
                     FSSVPLDSSPDLAVQLTIIENTDYYSAVYPVGYWWGAPGYFDPFYYGYNYPYTYGYYV
                     QNYSVSSGAVICELIDLKNANQQQALRIIWEATLLGAFEGSENYNINRSIAGFQQAFR
                     QSPYLKK"
     gene            1683..2300
                     /locus_tag="P5292_03620"
     CDS             1683..2300
                     /locus_tag="P5292_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38246.1"
                     /translation="MQRFLLLLLLILSGPRSMGQSDWIAQYGLSWNIATPIGRDFVEG
                     TTVSGGQVEFLVKPGKKPLMVGLQIEWSTYDTYTPTATYYFDNGNSAITTDYYAYQYN
                     LPLLACAQWYFGKSNRFFPWTRASMGGQYSLQQLYYGDFLEEYKSWGFAAKGEAGMSL
                     LVSQHLPLMISLSSGILYATNGAAELGDKDWMSWDVQLGFVFRQF"
     gene            complement(2393..3061)
                     /locus_tag="P5292_03625"
     CDS             complement(2393..3061)
                     /locus_tag="P5292_03625"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HRS38247.1"
                     /translation="MSLWNRLTGKSRTPEFPELTDFASIGTDIHSHLIPGIDDGVQNL
                     EESISMIRQLRELVFKKLVTTPHIMSDYFRNTPETILGGLETVRSAIVQEGIDLSIDA
                     AAEYYLDEVFVQRIEANEPLLKFAGNYILFEVSYVNPPDNLHRIAFELKIRGYSPILA
                     HPERYPYWYNRLTEFEKLKEAGLLFQLNANSLAGYYGPGAKRIGEWMIDQNMIDFIGT
                     DLHR"
     gene            complement(3058..4050)
                     /locus_tag="P5292_03630"
     CDS             complement(3058..4050)
                     /locus_tag="P5292_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS38248.1"
                     /translation="MYSSPFHPGSLRDTSFLVTGGSGFIGSNIVHYLVRHQAGKIRIL
                     DNLLTSSIANLEPYLNLPNVEFIEGDIRDLDTCHKACKGIDLVTHQAALGSVPRSVRD
                     PLATHAINATGFLNMLIAARDAGVKRFVYASSSSVYGDHPVLPKREDETGNPLSPYAV
                     SKKTNELYARVFAETYGMQIMGLRYFNVFGPNQSPDGPYAAVIPLFMKAALDKTAPNI
                     DGDGEQTRDFTFVENAVQANIRALFTPDPEANNRVYNVAIGERITVNELYDIIAELTG
                     SEVRPTYRERRAGDIRDSLADISLAKKHLGYQPTVRIREGLQQTLAWFRQTVGQ"
     gene            complement(4074..>4311)
                     /locus_tag="P5292_03635"
     CDS             complement(4074..>4311)
                     /locus_tag="P5292_03635"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRS38249.1"
                     /translation="PDGTSEKLITFVKDRAGHDLRYAIDASKLKHNLGWMPSLQFEEG
                     LEKTVAWYLANETWMEQVTSGDYLHYYEQQYAGR"
BASE COUNT          965 a         1085 c         1191 g         1070 t
ORIGIN      
        1 gaaccaccac tggggaaatt tgctttaaca ccggcatgac cgggtaccag gagatcttta
       61 cggatccatc ctatttccga caggtgctcg tggccacgca tgtccatatc ggcaactacg
      121 gcatccatgc ggacgaggtg gaaagtgacg ggatcaagat cagcgggctg gtctgtcgtg
      181 gattcaatgt tacgccttcc cggaagagtg cgagcgagag tattcagcag tatttcgacc
      241 gtcaatccgt cgtagccatt tgtgatatcg atacccgtgc agtcgtccgt cacatccgca
      301 ataaaggagc gatgaactgt atcatctctt cctcggtgct ggaccttgac gccttgaagg
      361 agcaactatc cggggtacct tccatggccg gcttggaatt gtcgagtctg gtgacgacca
      421 agaagcccta tacgttgggt aacccttccg cacgtcgtcg cattgcagtc atggattacg
      481 gagtaaagaa gaatatccta cgtaatcttg tcgagcgtga cagtttcctg caggtatttc
      541 ctgccgatac cagtttccgc gagatccgtt cgtggaatcc cgatggcata ctgctatcca
      601 acggccctgg cgatccttcc gtgatgccga aacaggtcgc cgagatcaag ttgatgttgg
      661 aagataatgc ccccatcttc ggtatctgtc tgggccacca attgctggcg gaggcttgcg
      721 gtatctctac ctttaagatg ttcaacgggc accggggtat caaccatccc gtcaagaacc
      781 tgatcaccgg tcgttctgaa atcacgtcgc agaatcacgg cttcagtgtg aatgccgaag
      841 acgtcaaagc tgccaaacac ctcgacatca cgcacgtcaa tctgaatgat gggacgatcg
      901 aagggatccg tgtgaaagac cgcgttgcgt ccagtgtaca atatcacccg gaagccgcgc
      961 ccggacctca cgacagctct tatctattcg atgattttat ccaatgcatc gacaaggccg
     1021 tggtcgagtc ctgatacccg gtaactccgg gatcttgccg gcgatcttct tttcgctggt
     1081 actagcactt tttacctcct gcagaaaaga accagcgact gatcaactgg cagagacctt
     1141 tacggtcctc acgaaccggg atccgggcgc ggatttcagt tcctattcaa ctttcttcct
     1201 ttcggatacg atcacgtata tcaacgacaa tccgtacgac agcatcctga ccggaccggg
     1261 cgccgggaat ctggcatcga gtgtcagaaa cgaattgatc agccgtggat tttcatcggt
     1321 gccgctcgat tccagccctg accttgctgt gcaattgacg atcatcgaga ataccgatta
     1381 ttactccgca gtttaccccg taggttattg gtggggtgca cccggttact ttgatccttt
     1441 ctattacggt tacaattatc catacacgta cggatactat gtacagaatt attccgtcag
     1501 cagcggggcc gtgatctgcg aactgatcga tctgaaaaac gcgaatcagc agcaagcttt
     1561 gcggatcatt tgggaagcga cactgttggg cgcattcgag gggagcgaga attataacat
     1621 caaccgctcg attgccggct tccagcaggc ttttcgtcaa tcaccctatt tgaagaagtg
     1681 atatgcagcg cttcctgctt cttctattgc tgatattatc cggaccgcgt tccatgggac
     1741 aatccgactg gatcgctcag tacggacttt cctggaatat cgcgacaccg atcgggagag
     1801 acttcgtgga ggggactaca gtgtcgggcg ggcaagtcga gtttctggta aagcccggta
     1861 agaaaccact gatggtcggc ctgcaaattg aatggtcaac ctacgatacc tatacgccta
     1921 ccgccaccta ttatttcgat aacggtaaca gcgcgatcac gaccgattat tacgcttatc
     1981 aatacaattt gccgctactg gcctgtgcgc agtggtactt tggaaaaagc aaccgatttt
     2041 ttccctggac ccgcgcttcc atgggcgggc aatacagctt gcaacaattg tattacggtg
     2101 acttcctgga ggaatataaa tcctggggat ttgccgccaa gggcgaggcc ggtatgagct
     2161 tattggtctc ccaacatctt ccgctgatga tcagcttgtc gagtggaatc ttgtacgcaa
     2221 ccaatggagc tgcggagctc ggtgacaagg actggatgtc ctgggatgta caactgggtt
     2281 ttgtattccg ccaattctga aaagcttaga gcaagctgtt ctttacgccg ttcgaagcca
     2341 gtttccagaa gtaccgttcc cgaacaactc gtcggagcgc ttcctggtgt cgttatctgt
     2401 ggaggtctgt gccgatgaag tcaatcatgt tctgatcgat catccattcg ccaattcttt
     2461 tagcccccgg tccatagtag cccgccaatg agttcgcgtt gagttggaac aggagtccgg
     2521 cttctttcag tttctcgaat tcggtcaggc gattgtacca gtagggataa cgttcagggt
     2581 gggcgagaat gggtgaatac ccgcgaatct tcagttcaaa agcgatccgg tggagattat
     2641 ccggcggatt gacataggat acttcaaaga ggatgtagtt gcctgcgaac ttcagcagcg
     2701 gctcattggc ttcgatgcgt tggacgaaga cttcatcgag atagtattcc gcagcggcgt
     2761 cgatgctgag gtcgatgcct tcctgcacaa tggcggatcg tacggtttcg agaccaccca
     2821 ggatcgtttc cggtgtgttc cggaaataat cactcatgat gtgcggggtg gtgaccaact
     2881 tcttaaacac caattcccgc aattgtcgga tcatggagat ggactcttcg aggttctgga
     2941 caccatcgtc gattccggga atgaggtggg aatggatatc ggttccgatt gaagcgaaat
     3001 ccgtgagttc cggaaattcg ggagtgcgtg atttaccggt cagccgattc cagagactca
     3061 ttgtccgacg gtttgacgga accaggccag tgtctgttga aggccttcgc ggatccggac
     3121 ggtgggttgg tatcccaggt gttttttagc gagcgaaatg tctgcgagcg aatcgcgaat
     3181 atcgccggcg cgacgttccc ggtaggttgg tcggacttca ctgccggtca gttccgcgat
     3241 aatgtcgtat agttcgttca ccgtaatgcg ttcaccgatc gctacattgt acacccggtt
     3301 attggcctcc ggatcagggg tgaacagcgc acggatgtta gcttgtacgg cattttccac
     3361 gaaggtgaaa tcacgtgttt gttccccgtc gccgtcgatg ttcggagcgg tcttatccaa
     3421 ggcggctttc atgaaaagcg ggatgaccgc cgcgtaggga ccgtcggggc tctggttagg
     3481 gccaaagacg ttgaagtagc gcagccccat gatctgcata ccgtaggttt cggcgaagac
     3541 ccgggcatag agctcgttcg tcttcttgct gacagcataa ggtgataacg gatttcccgt
     3601 ttcatcttcc cgctttggta agaccggatg atcaccgtac acggaactgc tgctggcata
     3661 aacgaagcgt ttcacgccgg cgtcacgcgc ggctatgagc atgttgagga atccggttgc
     3721 attgatcgca tgggtggcca gcggatcccg gacggaacga gggacggaac cgagggcagc
     3781 ctggtgtgtg accaggtcga ttcccttgca ggctttgtgg caggtatcga gatcccggat
     3841 gtcgccttcg atgaattcca cgttgggaag attcagatag ggttcaaggt tggctatgga
     3901 actcgtcagc aggttgtcga ggatgcggat ctttcccgcc tgatgtcgaa ccaggtaatg
     3961 gacgatattc gaaccgatga agccggagcc tccggtgacc aggaagctgg tatcgcgcaa
     4021 cgaaccggga tggaaaggag atgaatacat ggagtgggag ggcggataag cctttagcgg
     4081 ccggcgtatt gctgttcgta ataatgcagg taatctccgg acgtgacttg ttccatccag
     4141 gtttcattgg ccaggtacca ggctacggtt ttttccaatc cttcttcaaa ctgaagggag
     4201 ggcatccaac ccaaattgtg tttcaatttg gatgcgtcga tcgcgtatcg cagatcgtgt
     4261 ccggcacggt ccttcacgaa ggtgatcagt ttttccgagg tgccgtcggg g
//
LOCUS       DAOTON010000127         4308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16616_length_4308_cov_3.036210, whole genome shotgun sequence.
ACCESSION   DAOTON010000127 DAOTON010000000
VERSION     DAOTON010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16616_length_4308_cov_3.036210"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="P5292_03640"
     CDS             <1..704
                     /locus_tag="P5292_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38250.1"
                     /translation="LAKNNLKVTAAGWVCVAGLGRRTITTANAYQKMVKPGNGGLSCW
                     NSFIRVYKNDLSAPLYSSLIVGQWDTLTQQGGGNTELFGIFKTEGGVVGVGRQQADGS
                     GVPLGNDIPVTQVPSWGQSTPAGEAAILVFYPAGNLVNPLDNPGVTTGVADALSKSAF
                     VYPNPTTGMVSISGVWAAQSITLMDHTGRLLGSCNWSETGNGLRVTLPEVVEGAYLLS
                     VKSASGEHRFPFIIK"
     gene            complement(718..1764)
                     /locus_tag="P5292_03645"
     CDS             complement(718..1764)
                     /locus_tag="P5292_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRS38251.1"
                     /translation="MKRVLLYPVSLLLVLFSCKKDEGNFVAAGGPTPYSLVIPQGLPQ
                     LSIPVDNPLTVEGVSLGKRLFYDPILSGDNTISCASCHRQQYAFTDSTLQFSIGIDLL
                     PGTRNAMPLFNLGYARNFFWDGGATNLESQVIGPIMNPVEMHEDLANCIAELQAHPEY
                     PLLFYKAFKTDSITTALLMKAVAQFERTILSAGSKYDAVQRGQASFTAAEQRGLDIFT
                     DFQKGDCTHCHTLGSTFTDFEYRNTGLDSIAVDSGRARITLLPADLGRFKTPSLRNIE
                     LTAPYMHDGRFQTLQEVMDHYNTGFHSAANLDPNLRSATKGRMSQQDMDDVIAFLKSL
                     TDHSLLTNPAFQPD"
     gene            complement(1773..2534)
                     /locus_tag="P5292_03650"
     CDS             complement(1773..2534)
                     /locus_tag="P5292_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38252.1"
                     /translation="MNIRLLLLPAVFVLFLSSCKKDKDDTSDPTPTTTNKITVQVSNV
                     VDGSPVVFGQLIYTNAFGSSYSIDLLKYYLTNFRLVATDSSVHNFRNYDLINAADTTT
                     CNFAYSDIPERTYCKVRFYLGVDADSNHTGAQSGDLDPVNGMIWTWSTGYIFFKHEGQ
                     FLDTVGATQDLLYHFGTDAVLTEIEIPITPFTLGETDQKTIHLEFDLNGLYGSNTIVD
                     FNGNNIRQSLSVDDRPWMSDLMNNFPGAFRITSID"
     gene            complement(2773..3006)
                     /locus_tag="P5292_03655"
     CDS             complement(2773..3006)
                     /locus_tag="P5292_03655"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRS38253.1"
                     /translation="MKQLSKSLYAFLLLPVLMACNLPSSQKSSHEQLVVATDSTKAQW
                     HCPMNCQGDTSYTAAGSCPVCGMDLVEVEPKSK"
     gene            complement(3003..>4308)
                     /locus_tag="P5292_03660"
     CDS             complement(3003..>4308)
                     /locus_tag="P5292_03660"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRS38254.1"
                     /translation="HLHGKTMEQVLLGAAGMIIVRDSEEATLNLPHRYGIDDFPLALQ
                     WKTFDTLTNQLVMNDELDNAVMVNGAINGQLDVPAQMVRLRILNGSSHRFFYLAMNDN
                     RNFNVIAGDESLLNAPVSMNRLMVSPGERYEIVVDFSGQAGSTFFLRQLGTSLPAGYP
                     GGPPDGMGMMPMGPLDNTDFDIMRFNVQPTTADPITSLPAALTTNTVTPTTGAGSRSF
                     LMQGVPMMSMTNFTINGAQFDEGVINFQVEQDSVLIWNLTNQSMMPHPWHIHGNYFYI
                     NSIDGSAPPAHMQGRKDVVTVAPMGGTAQIIMKFEDFSDAMMPYMYHCHILSHEDNGM
                     MGQFLVTARTTDISMTGSATGVSSFPNPSNDSWTITGSSKDAAVRLSLLTITGVTVQQ
                     TLTSPVNGRFMQRMDASALPAGVYLLKIEENNTITTIKLLKR"
BASE COUNT          973 a         1040 c         1252 g         1043 t
ORIGIN      
        1 gccttgcgaa gaataatctt aaagtgactg ccgcgggttg ggtttgtgta gccgggcttg
       61 gacgccgtac gatcacgacg gccaatgcct accagaaaat ggtcaagccg ggcaacggcg
      121 gattgagttg ctggaacagc tttattcgtg tatacaaaaa tgacctgtca gctcccctgt
      181 acagttcact catcgtcggc caatgggaca cgctgacgca gcaaggcgga ggcaacaccg
      241 agcttttcgg aatcttcaaa acggaaggag gggtcgtagg tgtcggacga caacaggccg
      301 atgggtctgg agtcccactc ggaaatgata taccggttac acaggttcct tcctgggggc
      361 agtcaacacc tgcaggggag gctgcgatcc tcgtttttta tccggccggc aatctggtaa
      421 atccgttgga taatcctgga gttactacgg gcgttgctga tgcattatcc aaatccgctt
      481 tcgtctatcc caacccgacg actggtatgg tgagcatcag tggagtttgg gctgcgcagt
      541 cgatcacgct aatggatcat acgggcagac tactcggttc gtgcaactgg agtgaaaccg
      601 gaaatggctt acgcgtgacc ttgccggagg ttgtggaagg agcttacctg ttgtcggtga
      661 aatcagctag cggggaacac cgattcccgt tcatcatcaa gtaagtttat tcccgtatca
      721 atccggctgg aacgcgggat tcgtcagcag ggagtgatcg gtgagcgact tcaggaaggc
      781 gatcacatca tccatgtcct gttggctcat gcgacccttt gtcgcgctgc gcaggttagg
      841 atcgaggttg gcggcggaat gaaatccggt attgtaatgg tccatgacct cttgcaaggt
      901 ctggaaccgg ccgtcgtgca tgtaaggagc cgtcaattcg atgttccgta acgatggtgt
      961 cttgaacctt cccagatcgg caggcaggag ggtgatgcgg gcccgaccgg aatccaccgc
     1021 aatcgaatca agcccggtat tgcggtattc gaagtcggta aaggtgcttc ccagtgtgtg
     1081 gcaatgggtg caatcgcctt tctggaagtc ggtaaagata tccagtccgc gttgctcggc
     1141 tgccgtaaag gatgcctggc cccgttgcac ggcgtcatac ttcgatccgg ccgacaggat
     1201 ggtccgttcg aactgcgcga ccgctttcat cagcaaggcg gtcgtgatgg aatctgtttt
     1261 gaaagccttg taaaacagca acggatattc cggatgcgcc tgcaattcgg cgatgcaatt
     1321 ggccaggtct tcgtgcatct cgaccggatt catgatcggt ccgatcacct gtgattccag
     1381 attcgttgcc ccgccatccc agaaaaagtt ccgggcataa ccgaggttga agagcggcat
     1441 ggcattccgc gttcccggca gcaggtcgat gccaatgctg aattgcaggg tgctgtcggt
     1501 aaaagcgtac tgctgtcggt ggcaggaagc gcaggagatc gtgttgtcac cggaaaggat
     1561 cgggtcatag aacaggcgct tacccaagga gacgccttct acggtgagcg gattatcgac
     1621 cggaatcgac agttgtggca atccctgcgg aatcaccagg ctatagggag taggaccgcc
     1681 ggcagcgacg aaattacctt catccttttt acaggaaaac aggactaaga gaagggagac
     1741 cgggtagagc agtacgcgtt tcatgttccg gatcagtcga tggaggtgat gcgaaaagct
     1801 ccggggaagt tgttcatcag gtcgctcatc cacggtcggt cgtccacgct taaggattgc
     1861 cggatgttgt tgccgttgaa gtcgacgatc gtgttggacc catacaggcc gttcaggtcg
     1921 aattcaagat gaatggtctt ctggtctgtt tcaccaagcg tgaacggtgt aatcggaatt
     1981 tcaatttcag tcagtaccgc atcggtaccg aaatgataga gcaggtcttg ggtggccccc
     2041 acagtgtcca ggaactggcc ttcgtgcttg aagaaaatgt atccggtgct ccaggtccag
     2101 atcatgccgt taacgggatc gaggtcgccg ctttgcgcgc cggtatggtt gctgtcggca
     2161 tcgaccccca ggtagaagcg gactttgcaa tacgtccgtt ccggaatatc cgaataggcg
     2221 aaattgcagg tagtcgtgtc cgccgcgttg atcagatcat aattccgaaa attgtgtacg
     2281 gaagaatcgg ttgctacaag acggaaattg gtcaggtagt atttcagcaa gtcaatggaa
     2341 tacgaactgc cgaaagcgtt ggtatagatc aattgaccaa aaaccacagg cgaaccgtcc
     2401 accacattgc tgacctgtac ggtgatcttg ttggtagtgg tgggggtcgg atcggatgtg
     2461 tcgtccttgt cttttttgca cgatgacagg aacaagacga acacggcagg tagaaggagt
     2521 agccggatat tcatgacagg taaaggtagg ggattaagag tgggaagaca agtggttgtg
     2581 tgttgttgtc agagccttat tctctttttg aggttggttt gtccattcgg acttgatatc
     2641 aacggacggt ttaccaggaa ccgggcggct gccggcttcc gggtgagctc agagaaaggc
     2701 caatcgggcc gtcccgagaa gtaaacggat gatcggctcc tttcagatcg gagccgatca
     2761 tccgtacttt tcttatttcg attttggttc gacttctacc agatccattc cgcaaaccgg
     2821 gcaggagcct gcagcggtgt aggaggtatc accctggcaa ttcatcgggc agtgccattg
     2881 tgccttggtt gaatccgtcg cgacaaccag ctgttcgtga ctggatttct gactggacgg
     2941 caggttgcag gccatcaaaa ccggtagtaa cagaaatgcg tagaggcttt ttgacaattg
     3001 tttcatcgct tgagtagttt gatggtggta atcgtattgt tttcttcaat ttttaacagg
     3061 taaacaccag ccggtagtgc cgaagcatcc atgcgttgca tgaaccttcc gttgaccggg
     3121 gatgtcaagg tctgctggac agtaactccg gtaatggtca gcaggctgag gcggacggcc
     3181 gcgtcttttg aagaacccgt gatggtccaa ctgtcattcg acgggttagg gaaagagctt
     3241 acgcccgttg ccgatccggt catcgaaata tccgttgtgc gggcggtgac caggaactgt
     3301 cccatcatgc cattgtcctc gtgactcagg atgtgacaat gatacatata cggcatcatg
     3361 gcatcactga aatcttcgaa cttcatgatg atctgagccg ttccacccat tggagcgacc
     3421 gtcaccacgt ctttcctgcc ctgcatgtga gcgggcggag ctgacccgtc gatcgagttg
     3481 atataaaagt agttcccgtg tatgtgccac ggatggggca tcatgctttg attggtaagg
     3541 ttccagatca atacactgtc ctgttcgacc tggaagttga tgacaccttc atcgaattgc
     3601 gcgccgttga tggtgaaatt ggtcatgctc atcatgggaa cgccttgcat gagaaaggag
     3661 cgacttcctg caccggtggt gggtgtcacc gtattcgtgg tgagggcggc tggcagactt
     3721 gtaataggat cggcggtcgt tggttgaacg ttgaatcgca tgatatcgaa atccgtattg
     3781 tcaagcggtc ccatgggcat catacccatg ccatccggtg gtccgccggg atagcctgca
     3841 ggcaaactcg ttccgagttg acggagaaaa aatgtactgc cagcctggcc gctgaaatca
     3901 acgacgatct cgtaacgttc gcccggactg accattagcc tgttcatgct cacgggagca
     3961 ttcaaaagac tttcgtctcc ggcgatgacg ttgaaattac ggttgtcgtt catggccaga
     4021 tagaagaaac ggtggctact gccattcaga atacggagtc tgaccatctg cgccggtaca
     4081 tcgagctgtc cgttgatcgc tccgtttacc atcacggcgt tgtcgagttc atcgttcatg
     4141 acaagttggt tggtcaacgt gtcgaatgtc ttccattgca atgccagcgg gaagtcatcg
     4201 atgccataac gatgcggcag gttcaacgtg gcttcttccg aatcgcggac gatgatcatg
     4261 ccagccgcgc cgagcagcac ctgttccatc gtcttcccat gtaggtgt
//
LOCUS       DAOTON010000128         4288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16707_length_4288_cov_3.216631, whole genome shotgun sequence.
ACCESSION   DAOTON010000128 DAOTON010000000
VERSION     DAOTON010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16707_length_4288_cov_3.216631"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2407
                     /locus_tag="P5292_03665"
     CDS             <1..2407
                     /locus_tag="P5292_03665"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRS38255.1"
                     /translation="IPEDTLVSSEGLSLPVKYHARDSIRVDVANEAVYLYGAATVDYD
                     DLHLEAERIRINMGDKEVYAEGLTDSTGTPIGTPRFSQGPQEFRSRAMRYNFQTKKGK
                     IDYVITKEGDGYIHGEVVKKDPENNFFIRNGRYTTCELDTPHFSISSNKLKVISNNKI
                     VTGPAYLTIEQVPTPLFIPFGFFPNKKGRSSGIIFPGIGESAQRGFFFQHLGYYFGFN
                     DYFNLALTTDLYTKGSYTIDGISQYRKRYRYGGSFRVSYAYSVQSEKELPDFNARKDF
                     QITWNHNQDPKANPYSIFNASVHAGSSAYYQNTIASTNNFLANTFQSSLTWSKLFPDQ
                     PFNLSVAVNHSQNNLTRDVRITAPDVSFNVARIFPFQRKQQIGKQRWYEKIGFTYNLR
                     GTNYIQQKDSLLFRRESLDDMQNGLQHQIPISTAFNVLKFINVSPTFNYTERWYFRTT
                     EYAYNAEKGGLDTLTVNGFKAAREYSTGINALTRVYGMFQFAKGPVAAFRHVLTPSIG
                     FSYRPDFSQPNFGYFKTVQYDTSGNTLTYTIFQNSVYGGPSGGKFGSVNFGLDNNFEM
                     KVRTYSDTGMALKKIKLLESLRLGTNYNLIADSMNWSPLSISGRTTILDRAQITFNGS
                     LNPYAYDENNRYYNRFLKDVNGKLFQLTNANAAVNFSLLAKRKDKSSNKLSRDELNYI
                     NSHPEEYIDFEVPYNLYVGYNYGYSKFGNAASNTSQALSLSGDFSMTPRWKVGFNSWY
                     DIEAGRFTNFNLNINRDLHCWEFRFNWVPFGYQESYFFQINVKSSVLQDLKMMKQRTL
                     FDR"
     gene            complement(2561..3223)
                     /locus_tag="P5292_03670"
     CDS             complement(2561..3223)
                     /locus_tag="P5292_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38256.1"
                     /translation="MHPYWFRWIIFITAAGAAALSPATSYAGEGSLFRQENSGWFSLG
                     TRNTLSLFNEMPEEGMGVGIGGQFRLQLGKRLNTEWYFDYLTSEVGDFAQRNDYHIGW
                     SVMYYPGKHVDFSRLLQPYLILGHCFDYTDVFEKDNRSNHADRLSMATQGGIGTHFNI
                     TRALDVSLSGQYMVHFGKEVAADKQVGHTHIEVEDHTHAGGHVLLTVSANYKFVRLWG
                     RE"
     gene            complement(3266..3805)
                     /locus_tag="P5292_03675"
     CDS             complement(3266..3805)
                     /locus_tag="P5292_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS38257.1"
                     /translation="MAWTVEIAGVQDTEVVARIGRETFYETWRDYNTPEDMALYLQDA
                     FDHDKVRKDLANSEVNTFFLVRDGARIGGYAKVRRDRIYPALEGTRPLEIERVYVFRE
                     LHGEGLAATLMGAIIRLAQEEGMDMLWLGVNIDNARAIRFYRKYGFEIFGSKLFQLGK
                     ALDEDYLMKAAVPVLKQQQ"
     gene            3882..>4288
                     /locus_tag="P5292_03680"
     CDS             3882..>4288
                     /locus_tag="P5292_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38258.1"
                     /translation="MKKLLLPFLLATGLSVSAQKDNQMAPVKAPQPVKPVNPILRGEQ
                     VSGKIDTIATPDPLTPGRSQWTILNPNGSVKAQGFMRDGKRDGVWREYASSNNVLVKV
                     EEYRDGHKNGAVLAFSAVGQVNLNETYLNDTLQG"
BASE COUNT         1102 a         1245 c          979 g          962 t
ORIGIN      
        1 catccctgaa gatacgctgg taagctcgga aggactctcc cttccggtca agtatcacgc
       61 cagagactcg atccgggtgg acgtagcaaa cgaagcggtg tatttatatg gcgctgcaac
      121 ggtcgattac gatgacttgc acctcgaagc ggaaaggatc cgcatcaaca tgggcgacaa
      181 ggaagtctat gctgaagggc tgaccgatag caccggtaca ccgatcggca ctccccgctt
      241 ctcgcaagga cctcaggaat tccggtcccg tgccatgcgg tacaacttcc aaacgaaaaa
      301 aggcaagatt gattatgtaa tcaccaaaga aggagacggt tatatccacg gtgaggtcgt
      361 caaaaaagac ccggaaaaca actttttcat ccgcaacgga cggtacacta cctgcgaact
      421 cgatacacct catttttcca tatcttccaa caagctgaag gtcatcagca acaataagat
      481 cgtcacaggc cccgcttacc tgacgattga acaggttccg acccccttgt tcattccctt
      541 cggtttcttt ccgaacaaga aaggtcgcag ctccggtatc atttttccag gcatcggcga
      601 atccgcccaa cgcgggttct tcttccagca tctgggatac tacttcggct tcaacgacta
      661 tttcaacctg gccctgacca ccgacctgta caccaaaggc agctacacga tcgacgggat
      721 ttcacaatac cggaagcgtt atcgttatgg tggctccttt cgggtgagct atgcctactc
      781 ggtacaaagt gaaaaggaac tgcccgactt caacgccagg aaggattttc agatcacctg
      841 gaaccacaac caggacccca aagccaatcc ttacagcatc ttcaacgcca gtgtacacgc
      901 cggctcctcg gcctactatc aaaacacgat cgccagtacg aataactttc tggcgaatac
      961 cttccagtct tccctcacct ggtcgaaatt gttcccggac caacccttca acctcagtgt
     1021 agccgtcaac cattcccaaa acaacctcac ccgggatgta cggatcaccg cgccggatgt
     1081 cagtttcaat gtggcccgta tctttccttt ccagcgcaag caacagatcg gcaagcaacg
     1141 ctggtatgag aagatcggct ttacctacaa cctgcgcggc accaactata ttcagcaaaa
     1201 ggatagcctg cttttccgac gtgaatcgct ggatgacatg caaaacggcc tgcagcacca
     1261 gatcccgatc tcaacggctt tcaacgttct caaattcatc aacgtctccc cgaccttcaa
     1321 ctataccgag cgctggtatt tccgcacgac cgaatatgcc tacaacgcag aaaaaggcgg
     1381 actcgacacg ctcacagtga atggcttcaa agccgccagg gaatattcta ccggaatcaa
     1441 tgcgttgaca cgtgtttacg gcatgttcca gttcgccaaa ggtcctgtgg cggctttccg
     1501 gcatgtactg actccttcaa taggattcag ttaccgtcct gatttctccc aaccgaattt
     1561 cggctacttc aaaaccgttc agtacgatac cagcggaaat acactcacct acacgatctt
     1621 ccagaatagc gtttacggcg gtccctccgg cgggaaattc ggcagcgtaa atttcggtct
     1681 cgacaacaac tttgagatga aagtgcggac ctactccgac accggcatgg cgctgaaaaa
     1741 gatcaaactg ctggagagcc tgcgcctggg aacaaattat aacctgatcg ccgattccat
     1801 gaactggagt ccgctgtcga tcagtggtcg caccacgatc ctcgaccgcg cccagatcac
     1861 gtttaacgga tcactgaacc cctatgccta cgatgagaac aaccggtatt acaaccgttt
     1921 cctcaaggat gtcaatggca agctcttcca attgaccaat gcgaatgctg ccgtgaactt
     1981 ctcgctgctg gcaaagcgca aagacaagag cagcaataaa ctcagtcggg acgaactcaa
     2041 ttacatcaac agtcaccccg aggagtacat cgacttcgaa gtaccctaca acctgtatgt
     2101 cggttacaac tacggttatt cgaaattcgg caatgctgcc agtaacacct cgcaggcact
     2161 cagcctgagc ggcgatttca gcatgacacc ccgctggaaa gtcggcttca actcctggta
     2221 cgatatcgaa gcaggccgtt tcaccaactt caacctcaac atcaaccgcg acctgcactg
     2281 ctgggagttc cgcttcaact gggttccctt cggctaccag gaaagctact ttttccagat
     2341 caacgtaaag tcatccgtcc tccaggacct caaaatgatg aagcaacgga cgctttttga
     2401 caggtagggg tggggattag aaattagtaa ttagtaattt gtaatttgtg atttgtgatt
     2461 gttgattggg gagagttgaa agatgaaagt ggaaagttga aggatcactg ttcacaattc
     2521 actattcgct attcgctaat cgctattggc tgctcactgt tcactccctt ccccacaacc
     2581 gcacaaactt ataattcgcc gaaacggtca acaatacgtg tccgcccgcg tgggtatggt
     2641 cctctacttc gatgtgagtg tgaccgacct gcttgtctgc cgctacctct tttccaaaat
     2701 gcaccatgta ttgcccggag aggcttacat ccagcgctct cgtgatgttg aagtgggtcc
     2761 cgataccgcc ttgcgtcgcc atactcaggc gatcggcatg attgctccgg ttgtcttttt
     2821 caaaaacatc cgtgtaatcg aaacaatgcc ccagaatcag gtacggctgt agcaagcggg
     2881 aaaaatcaac gtgcttaccg ggataataca tcaccgacca accgatgtgg taatcgttcc
     2941 gctgagcaaa atcgcccacc tccgacgtca gataatcgaa gtaccattcg gtattcaaac
     3001 gcttacctaa ttgcaaccgg aactggccgc cgatgccaac ccccatgcct tcttccggca
     3061 tctcgttgaa caggctaagc gtgttgcgcg taccaagtga aaaccaaccg gaattttcct
     3121 gtcggaagag actcccttct ccggcatagc ttgttgcagg actcagtgcg gccgcccctg
     3181 cagccgtgat gaagatgatc caacgaaacc agtaaggatg catggggtct gtgatcttcg
     3241 ggtaataacg ctgataagct gatgtttatt gttgttgctt caacactggt accgcagcct
     3301 tcatcaggta atcttcgtcc aacgctttgc cgagctgaaa cagtttactg ccaaagatct
     3361 cgaaaccata tttccggtaa aagcggatcg cgcgcgcgtt gtcgatgttg acccctagcc
     3421 aaagcatatc catgccctcc tcttgcgcaa gacgaatgat cgcacccatg agcgtcgccg
     3481 ccaatccttc cccatgcaac tcccggaaga cgtacacccg ctcgatctcc aacggacgtg
     3541 ttccttccaa agcgggatag atccggtcgc gacgaacctt ggcgtagcca ccgatccgcg
     3601 caccatcacg taccaggaag aaagtgttca cttctgaatt ggcgagatcc ttccggactt
     3661 tatcgtggtc aaaggcatct tgcagataga gcgccatatc ctccggggta ttgtaatccc
     3721 gccatgtttc ataaaaggtc tcccgaccga tccgggcgac aacctccgtg tcttgaactc
     3781 cggcaatttc tactgtccag gccatgcggt gaaaatacgc agcttccgat gttcatcaca
     3841 aattcccgaa gcaggactat ctttgcgccg gtttaactgc tatgaaaaaa ctcctgctcc
     3901 ccttcctgct cgcaaccggc ctgagtgtct cggctcagaa agacaatcaa atggccccgg
     3961 tcaaggctcc tcaaccggtc aaaccggtga acccgatcct gcgtggagaa caggtttctg
     4021 gaaagatcga taccattgca acgcccgatc ccctgactcc tggtcgcagc cagtggacca
     4081 tcctcaaccc caacgggtcc gtaaaagcgc agggtttcat gcgcgacggc aagcgggacg
     4141 gtgtctggcg cgaatatgcc agtagcaaca acgtgctggt aaaagtcgaa gaatatagag
     4201 atgggcacaa gaacggcgcc gtacttgcct tttccgccgt cggccaggtc aacctgaacg
     4261 agacctacct caacgatacc ctccaggg
//
LOCUS       DAOTON010000129         4279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16742_length_4279_cov_3.001420, whole genome shotgun sequence.
ACCESSION   DAOTON010000129 DAOTON010000000
VERSION     DAOTON010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16742_length_4279_cov_3.001420"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /gene="rpsM"
                     /locus_tag="P5292_03685"
     CDS             complement(<1..248)
                     /gene="rpsM"
                     /locus_tag="P5292_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRS38259.1"
                     /translation="MARIAGIDLPKNKRGEIGLTYIYGIGHTSAQRILREAGIDFNTK
                     VSEWNDDQLNKIRSIINEMKVEGALRSETQLNIKRLVD"
     gene            complement(285..401)
                     /gene="rpmJ"
                     /locus_tag="P5292_03690"
     CDS             complement(285..401)
                     /gene="rpmJ"
                     /locus_tag="P5292_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRS38260.1"
                     /translation="MKVKPAIKKRSVDCIIVRRKGRLYVINKKNPRFKQRQG"
     gene            complement(441..662)
                     /gene="infA"
                     /locus_tag="P5292_03695"
     CDS             complement(441..662)
                     /gene="infA"
                     /locus_tag="P5292_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRS38261.1"
                     /translation="MAKQANIEQDGKITEALSNAMFRVELENGHEIIAHISGKMRMHY
                     IKILPGDKVKVEMSPYDLTKGRITYRYKR"
     gene            complement(667..1464)
                     /gene="map"
                     /locus_tag="P5292_03700"
     CDS             complement(667..1464)
                     /gene="map"
                     /locus_tag="P5292_03700"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRS38262.1"
                     /translation="MLYYKTLEEIELIRQSSLLVAKTLAEVAKSIRPGITTIELDRIA
                     ETFIRDQGAVPAFLNYNGFPNSLCISVNSQVVHGIPGKAELKDGDICSVDCGVLKDGF
                     YGDSAYTFPVGDVAASTLNLLRITRECLYEGIAKAVAGNRLGDISSAIQEYAESNGYS
                     VVRELVGHGIGRHLHEKPEVPNYGKRGSGLVLREGLVIAIEPMINMGTKGVVHERDGW
                     TIRTADNKPSAHFEHTVAIGKEKADILSSFDYVEEALKTNTNIKVIN"
     gene            complement(1515..2873)
                     /gene="secY"
                     /locus_tag="P5292_03705"
     CDS             complement(1515..2873)
                     /gene="secY"
                     /locus_tag="P5292_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRS38263.1"
                     /translation="MKNLIQTIKNIFKIEDLRVRILNTLLFLLVYRLGSHIVLPGVDP
                     LQLDALRQQTSGGVLGLLDMFSGGAFSHSSIFALGIMPYISASIVIQLLSMAVPYFQK
                     LQKEGESGRKKINNITRWLTVIITGFQAPGYIVNLQAQVPSAIISVSNPELVSPFQFW
                     LTSIVILTAGTIFVMWLGERITDKGIGNGISLIIMIGIIARLPASFRDEFASRMNQGG
                     GLVVLLIELVALAAVIAFVILLVQGTRRIPVNFAKKIVGNKQYGGVRQYIPLKVNAAG
                     VMPIIFAQAIMFIPATIAQYFPESSASQGILTTFGNYTSFWYNFVFATLIILFTYFYT
                     AIAVNPNQMADDMKRNNGFIPGVKPGRSTADFIDDVMSKITLPGAIFLALIAILPTFA
                     MLLNVNNNFAHFYGGTSLLIMVGVVLDTLQQIESHLLMRHYDGLMKSGRIKGRSTVAS
                     AV"
     gene            complement(2870..3322)
                     /gene="rplO"
                     /locus_tag="P5292_03710"
     CDS             complement(2870..3322)
                     /gene="rplO"
                     /locus_tag="P5292_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRS38264.1"
                     /translation="MNLSSLKPAKGSVRNNVRRGRGTGSGMGGTATRGHKGAQSRSGY
                     SAKRGFEGGQMPLQRRVPKFGFKNPFRKEYNGINLDTLQKLAEAKKLSSIDFASLLES
                     GLVSKRDRVKILGRGELKSKLDVKAHAFSASAKAAIEAKGGTTTIIEA"
     gene            complement(3352..3531)
                     /gene="rpmD"
                     /locus_tag="P5292_03715"
     CDS             complement(3352..3531)
                     /gene="rpmD"
                     /locus_tag="P5292_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRS38265.1"
                     /translation="MAKVKITLVRSGIDRPESQKRTLKALGLTKMHRSVEVEATAQIR
                     GMINKVQHLVEVKEA"
     gene            complement(3552..4070)
                     /gene="rpsE"
                     /locus_tag="P5292_03720"
     CDS             complement(3552..4070)
                     /gene="rpsE"
                     /locus_tag="P5292_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRS38266.1"
                     /translation="MSETNVKRVKSSEIELKDRLVGVNRVTKVTKGGRAFTFAAIVVV
                     GDESGVVGHGLGKAKEVSDAVQKGIDDAKKNLVKVPVMKGTIPHEAHGKFGGAYVFLK
                     PAAPGTGVIAGGAMRAVLESVGIHNVLAKSKGSSNPHNVVKATIDALAQMRDPATVAQ
                     QRGIKMARVFNG"
     gene            complement(4104..>4279)
                     /locus_tag="P5292_03725"
     CDS             complement(4104..>4279)
                     /locus_tag="P5292_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRS38267.1"
                     /translation="TATKGPKVDLAREVGKKIAEKAVQSGIKTCVFDRGGYLYHGRVK
                     ALAEGAREAGLQF"
BASE COUNT          897 a         1198 c         1139 g         1045 t
ORIGIN      
        1 atatccacca gacgcttgat gttcagttgc gtctccgaac ggagcgcacc ttccaccttc
       61 atctcgttga tgatcgatcg gatcttgttg agttggtcgt cgttccactc cgacaccttg
      121 gtgttgaagt cgataccggc ttcccggagg atccgttgtg cagaggtgtg accgatcccg
      181 tagatgtagg tcagcccgat ctctccgcgt ttgttttttg gtaaatcgat accagcaata
      241 cgtgccatta tcttgaggag tgtttagttg ttgaagtaca ggaatcagcc ctggcgctgt
      301 ttgaaacgcg ggttcttctt gttaatgaca tacaagcggc ccttgcgtcg cacaatgatg
      361 cagtctacgc tgcgcttctt gatggcgggt tttactttca tcgctttgtt tgctcttcat
      421 gatcccggat taggggatcg ttatcgttta tagcggtaag tgattcttcc tttcgtcagg
      481 tcgtaaggcg acatttccac cttgacttta tccccgggca ggatcttgat atagtgcatt
      541 ctcatcttac cggaaatatg ggcgatgatc tcgtgaccgt tctccaattc cacgcggaac
      601 atggcgttgg agagggcttc ggtgatcttc ccgtcctgtt cgatatttgc ttgtttggcc
      661 aacttgtcag ttgataacct taatgttagt attcgttttg agcgcttcct ccacgtagtc
      721 gaaagaactc agtatgtctg ctttttcctt gccaatcgcg accgtgtgct cgaagtgcgc
      781 cgacggtttg ttgtccgccg tccggatggt ccagccatcc cgctcgtgca ccaccccctt
      841 cgttcccata ttgatcatgg gttcgatggc gatgaccagc ccttcgcgca acaccagccc
      901 actcccccgc ttgccgtaat tcggcacctc gggtttctcg tgcaggtggc ggccgatccc
      961 gtgaccgacc agttcgcgca ctacggagta accgtttgat tcagcatact cctgaatggc
     1021 ggagctgata tccccgagcc ggtttcccgc gactgctttc gcgatccctt cgtacaggca
     1081 ttcccgcgtg atgcgcaaca ggttcagggt cgatgccgct acatccccta ccggaaatgt
     1141 ataagcggaa tctccataga acccatcttt caacacacca caatcgaccg agcagatatc
     1201 accatccttc aattccgcct ttcccggaat gccatgtacc acctgcgaat tcacagagat
     1261 acaaaggctg ttgggaaagc cattgtaatt gagaaaggct ggaacggcac cctggtcacg
     1321 aataaacgtt tccgcgatcc ggtccaactc aatcgtcgtt atgcccggcc ggatggattt
     1381 cgccacctcg gcaagggtct tggcaacaag taaagaactc tgcctgatca gttcgatctc
     1441 ttcgagcgtc ttatagtaca gcatcgctct tggtcggctc cccggatttt tgacgcccgg
     1501 gaaacctgat caggttaaac agcggaagca acggtcgaac gacccttgat ccgcccactc
     1561 ttcatgagcc cgtcgtaatg acgcatcagc aggtggctct caatctgctg aagtgtatcc
     1621 agtactacac ccaccatgat cagcagggag gtcccgccgt aaaagtgagc gaagttgttg
     1681 ttgacattca ataacatcgc aaaggtcggc aaaatcgcga tgagggcaag gaagatcgca
     1741 cccggaagcg tgatcttcga catgacgtca tcaatgaaat ccgccgtgct tcgacccggc
     1801 ttcacacccg gaatgaatcc gttgttccgc ttcatgtcat ccgccatctg gttcgggttg
     1861 acggcgattg ccgtgtagaa gtaggtgaac aggatgatca gcgtagcgaa tacgaagttg
     1921 taccagaacg aagtgtagtt tccgaaggtg gtcagaattc cctgcgaagc agaggactcc
     1981 gggaaatact gggcgatcgt tgccgggatg aacatgatcg cctgcgcgaa gatgatcggc
     2041 atgacaccgg ctgcattcac tttcaagggg atatactgac gtacaccgcc gtattgctta
     2101 ttgccgacaa tcttcttggc gaaattcacc ggaatgcgcc gcgtaccctg caccagcagg
     2161 atcacgaagg caatcacggc agcaagcgct accagttcga tcaggagcac gaccaatccg
     2221 ccaccctggt tcatgcgcga tgcgaattcg tcgcggaacg acgcgggcag tcgggcgatg
     2281 atgccgatca tgatgatcag ggaaataccg ttgccgatac ccttatccgt gatccgctca
     2341 cccagccaca tcacgaagat ggtcccggcc gtcagaatga cgatggaggt cagccagaac
     2401 tggaacggcg acaccaattc cggattggat acggagatga tggccgaagg aacctgagcc
     2461 tgcaggttaa cgatgtaacc aggggcctgg aagccggtga tgatcaccgt cagccaacgg
     2521 gtgatattgt tgatcttttt acgaccgctt tcgccttcct tctggagctt ctggaagtac
     2581 ggaactgcca tactcaacag ctggatcacg atcgaagcgg agatgtacgg catgataccg
     2641 agcgcgaaga tcgacgagtg cgagaacgcg ccgccggaga acatgtcgag aagacccaag
     2701 acaccgccgg acgtctgctg acgcagggca tccagttgca acggatccac acccggaagc
     2761 acaatgtgcg aaccgaggcg gtataccagc aggaacagca gggtgttcag gatccggacc
     2821 cggaggtctt cgatcttgaa gatattctta atcgtctgga tcaggttctt catgcctcga
     2881 tgatggtcgt ggttcctcct ttagcttcaa tggctgcctt tgcggaagcg gagaaggcgt
     2941 gcgccttgac atccagcttg gacttcaact ctccgcgacc gaggatcttt acacggtcac
     3001 gcttgctgac caatccgctc tccaacaggg aggcgaaatc aatcgaactg agtttcttgg
     3061 cttcagccag cttctggagc gtgtccaggt tgatgccgtt gtattccttg cggaacggat
     3121 tcttgaaacc gaacttcgga acacgacgct gaaggggcat ctggccgcct tcaaatccac
     3181 gcttcgcgct gtagccggag cgggattggg cgcctttgtg accgcgtgta gcggtaccgc
     3241 ccataccgga accggtacca cggccgcggc gtacgttgtt tcgaacggaa cctttcgcag
     3301 gtttaaggga cgacaaattc atgagaaggt ctttcttcgg attgttagtg attaggcttc
     3361 cttcacctct accaggtgct ggaccttgtt gatcatgccc cggatctggg cggtggcttc
     3421 cacctcgacg ctgcggtgca tcttcgtcag gccaagtgcc ttcagcgtac gcttctggct
     3481 ctcgggacgg tcaatgccgc tacggacaag ggtgatttta acttttgcca tgggattcgt
     3541 atagagggtt cttaaccgtt gaatactcgg gccatcttga tgccacgttg ctgagcaacc
     3601 gtagccggat cacgcatctg cgccagcgcg tcgatcgtag ctttcaccac gttgtgcggg
     3661 ttggaactac ccttcgactt cgccagtacg ttgtggatgc ctacgctctc cagaacggca
     3721 cgcattgcac cgccggcgat tactccggta ccgggtgctg ccggcttcag gaacacgtat
     3781 gcgccaccaa acttaccgtg cgcttcatgc ggaatggtgc ctttcatgac cggcactttt
     3841 accaggttct tcttcgcgtc gtcgatgcct ttctgtacag catcggatac ttctttcgct
     3901 ttgccaaggc cgtgaccgac tacaccggac tcgtctccta ccaccacgat ggcggcaaag
     3961 gtgaaggcgc ggccaccttt ggtcaccttg gtgacccggt tcacgccgac caggcgatcc
     4021 ttcagttcga tttcgctcga cttgacgcgt ttaacgtttg tttcagacat ggtttgtctt
     4081 cctaatgaga atgatcgtat cgtttagaat tggagtccgg cttcgcgggc cccttcggcc
     4141 agggctttca cccggccatg gtacaggtaa ccgccacggt cgaacacgca ggtcttgatg
     4201 ccgctctgga cagccttctc ggcgatcttc ttgccgactt cgcgggccag atcgaccttc
     4261 ggtcctttgg ttgcagtca
//
LOCUS       DAOTON010000130         4261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16824_length_4261_cov_4.964099, whole genome shotgun sequence.
ACCESSION   DAOTON010000130 DAOTON010000000
VERSION     DAOTON010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16824_length_4261_cov_4.964099"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1008
                     /locus_tag="P5292_03730"
     CDS             97..1008
                     /locus_tag="P5292_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38268.1"
                     /translation="MATEITTQEILQEAGKTKFRTILLTVLLSFATFYFCRFHLFLYQ
                     SEAKVTWVDPTVVDARIIEDQGANPFKSDFYNRLFATVHSNDMYRHLIRKFGLLQHYG
                     IDTTKEFYEERAIRILKSRIKLVKTPFNLIIISVEDAYRYRCAEIANEAATYADELNR
                     KNTVTALRKRLRINEEMIGKLRAEHDAQAQRLQKSLDELEGLPHSATMDRSQEALAQT
                     IYALHSSTDQLINAGKYYRLLEASLLDRNLPSIVTVESARASASSLNGKAGLIAGLVA
                     IGCFLFALLSTYFRLRYQHLAKAYLRG"
     gene            1019..1705
                     /locus_tag="P5292_03735"
     CDS             1019..1705
                     /locus_tag="P5292_03735"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRS38269.1"
                     /translation="MRKILILVAGGSGSRMGSDVPKQFLLLNGKPVLLHTLDQAHQAD
                     PALEIVVVLPATQLERWNALCREHACAVSHQTVAGGATRYESVRNGLSAVSASITSPA
                     QTVIGIHDGVRPYLPKEFLQRCFSEASEKGTAIPCLPVTDSLRQQIGDRYSAVDRSRF
                     FLMQTPQCFRADLGLRAYAGEEQRGFTDDASVLEQANIPLHFVAGERWNIKITYPGDL
                     AVAAALLAGK"
     gene            1797..2765
                     /locus_tag="P5292_03740"
     CDS             1797..2765
                     /locus_tag="P5292_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRS38270.1"
                     /translation="MKTFLRILKRIGLILLVILVLFVAVVYIRADRTFEAPLPEISAS
                     RDSAVIARGEYLVYGPAHCADCHTSRDQRADVLAGKHVPLHGGGYFDLPFGRVYTHNL
                     TPDATGLLSFSDYEIARSLRYGVAKDGHALLDFMPFHNLSDEDLTAVISFLRTLEPVK
                     YEVPKTEFNFIGKAIKAFMIKPIGPDGEVAKSVTPALSAEYGKYLANSIANCRGCHTN
                     RSLATGEYTGPFFAGGFHMPVDDKPGVFVVSPNLTPDPETGHIAKWSEDDFVKRFREG
                     KKIPDSPMPWGPFSNFSDDDLRSIYRFLHTIEPVKQVNGPTIVVEN"
     gene            complement(2880..3947)
                     /locus_tag="P5292_03745"
     CDS             complement(2880..3947)
                     /locus_tag="P5292_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HRS38271.1"
                     /translation="MENVIRQCIGIDCGMKEHVASFSRMYADLRVEYVSTLTIPNGPG
                     GFVKLSKWIRKLMDPSMPCQFVLEATGVYHETLACFLTEKGWPVSVVLPNRAKYFSKT
                     LTVKTVNDKVSSQTLAAMGLEKKLDVWSPPDPIYNQLRQLTRERDQLKAESTQIKCQL
                     HAEESGAWPNKSSIKRMNQRLSLIERQVEEVETSIREVVAESPEIDQAVRRMTSIVGV
                     GMLTAVTVLAETNGFNLVRNKKQLVSYAGLDVVERTSGISVRGRSRISHRGNRYLRKC
                     LYFPAMTAARHSNDHKALYNRLISKHGIAMKAAVAVQRKLLVMMYAIWKNGTIYDPNY
                     ESKKQQNELGQTRRPALTELA"
BASE COUNT          913 a         1243 c         1110 g          995 t
ORIGIN      
        1 gcggtgacca acgcgaagaa acggcgctca tggaaacgga accgatgata cgattttttt
       61 ggtttgtcgt acgggtcttt ctatacttac agaagaatgg cgacggaaat caccacacag
      121 gaaattctgc aggaggccgg gaagaccaaa ttccggacga tcctcctcac cgtcctgctc
      181 tccttcgcca cgttttattt ctgtcgtttc catcttttcc tctaccagtc ggaagcgaaa
      241 gtgacctggg tggacccgac ggtcgttgat gcacgcatca tcgaggatca gggcgccaat
      301 ccgttcaaaa gcgatttcta caaccggttg ttcgccacgg tgcactcgaa cgacatgtac
      361 cggcacctca tccggaagtt cggcttgttg caacactatg gcatcgatac caccaaagaa
      421 ttctacgaag agcgcgccat ccggatcctg aagtcccgta tcaagctggt caagacgccg
      481 ttcaacctga tcatcatctc ggtggaagac gcttaccgct atcgctgcgc cgagatcgcg
      541 aacgaggccg ccacctatgc tgacgagctc aaccggaaga acaccgtcac cgcgctgcgc
      601 aaacgactgc gcatcaacga agagatgatc gggaagctgc gcgcggaaca cgatgcccag
      661 gcacagcgtc tgcagaagag cctggatgaa cttgaaggcc ttccccactc cgcgacgatg
      721 gaccgttcac aggaggcgct ggctcagacg atctacgctt tgcacagctc gaccgatcag
      781 ctcatcaacg ccggcaagta ctaccgactc ctcgaagcct cgctcctcga ccgcaacctg
      841 cccagcatcg tcacggtgga gtcggcgcgg gccagcgcgt cttcgctgaa cggaaaggcc
      901 ggactcatcg ccggtctcgt cgcgatcggc tgtttcctgt tcgcgctcct ttccacttat
      961 ttccgcttgc ggtaccagca cctcgcaaag gcgtatcttc gcggctgagt tcagcacgat
     1021 gcgcaagatc ctgatcctgg tcgccggagg ctccggaagc cgcatgggaa gcgatgttcc
     1081 caagcagttc ctcctgctca acggcaaacc ggtcctgctg catacgctcg accaggcaca
     1141 ccaggccgat cctgcgctgg agatcgtcgt cgtacttccc gccactcaac tggaacgctg
     1201 gaacgcgctc tgccgcgaac acgcttgcgc ggtgagccat caaaccgtcg ccggcggcgc
     1261 gacccgctat gagagcgtcc gcaacggact ctccgcggtc agcgcctcca tcacctcgcc
     1321 cgcgcaaacg gtgatcggca ttcacgatgg tgttcgacct tacctgccga aagaatttct
     1381 ccagcgctgc ttttctgaag ccagcgaaaa aggaaccgcc attccctgcc tgccggtgac
     1441 cgactcgctg cgacagcaaa tcggtgatcg ctactcggcc gtcgatcgct cccgcttttt
     1501 cctgatgcag acgccgcagt gttttcgcgc cgacctcggc ttgcgcgctt acgcgggtga
     1561 agaacaacgc ggcttcaccg acgatgcttc ggtgttggaa caagccaaca tccccctgca
     1621 cttcgtggcc ggtgagcgtt ggaacatcaa aatcacgtac ccgggcgatc ttgccgttgc
     1681 cgccgcgctg cttgccggga agtaggattt ttctccgaaa aaattgaccg atttccggct
     1741 gttgttcaat gcaaggaatt gcattctaat tttctattcc ccccaactct ttgatcatga
     1801 aaacgttcct tcggatcctt aaaaggatcg gtttgatcct gcttgttatt ctggtattgt
     1861 ttgtcgctgt tgtttacatc cgggccgacc ggacttttga ggccccactg ccggagatct
     1921 ccgcgagccg cgacagcgcc gtaatcgcac ggggcgaata cctggtgtac ggtccggcac
     1981 actgcgccga ctgccatact tcccgcgacc agcgggcgga tgtgctcgcc ggtaagcatg
     2041 tgccgctgca cggcggcggc tatttcgatc tgccgttcgg cagggtgtac acgcacaacc
     2101 tgaccccgga tgcgacgggt ttgctttcct tcagcgacta cgagatcgcg cgctcgttgc
     2161 ggtatggtgt cgcgaaggac ggacacgcgc tgctcgactt catgccgttc cacaacctga
     2221 gcgacgagga cctgacggcc gtgatctcct ttctccgtac actggaaccg gtgaagtacg
     2281 aggtgccgaa aaccgaattc aatttcatcg gcaaggcgat caaggctttt atgatcaaac
     2341 cgatcgggcc ggatggcgag gtggcgaaaa gcgtgacgcc tgctttgagt gcggaatacg
     2401 gaaaatacct ggcgaacagc atcgcgaact gccgcggttg tcatacgaac cggagtctgg
     2461 cgacgggtga atatacagga ccgttcttcg cgggcggatt ccatatgccg gtcgatgata
     2521 aaccgggcgt gttcgtggtt tcaccgaacc tgacgccgga tccggagacg ggtcatatcg
     2581 cgaagtggag cgaggatgat ttcgtgaagc gtttccggga ggggaagaag atccccgatt
     2641 cgccgatgcc ctggggaccg ttctccaact tcagcgacga cgacctgcga tcgatctatc
     2701 gcttcctgca taccatagaa cccgtcaaac aagtgaatgg cccgacgatc gtggtggaga
     2761 actgagtacg ctaaaaaaga gtagcacaga gtaacgcgga gggcacacag agattctgtg
     2821 ttaaccgcca aatttccgtg ttagattttt ttctaaattc gggattacaa agaacggctt
     2881 taagccagtt ccgttagggc aggtcttcgg gtctgcccta attcattttg ttgctttttt
     2941 gattcgtagt tggggtcgta gattgttccg tttttccaga tcgcatacat cataaccaat
     3001 aatttccgtt gaaccgccac cgctgctttc atcgcaattc cgtgtttgct gatgagcctg
     3061 ttgtacaacg ccttatggtc atttgaatga cgtgctgcag tcatggccgg gaagtacagg
     3121 cactttctta agtaccggtt ccctcgatgc gatatgcggg accttcccct gaccgatata
     3181 ccggatgtcc tttccaccac atccagcccc gcatagctga ctagctgttt cttgttcctt
     3241 accagattga atccattggt ttccgccagc accgttactg ctgtgagcat cccaacacct
     3301 acaatcgatg tcatgcgtct tacagcctga tcaatctcag gtgattcagc aacgacttcc
     3361 cggatggacg tttccacttc ttctacctgt cgttcaatca aagacaatcg ttgattcatc
     3421 cgtttaatgc ttgacttgtt gggccaggct ccgctctctt ctgcgtgtaa ctgacatttg
     3481 atctgcgtac tttccgcttt gagctgatct cgctcccggg ttagctgccg cagctgattg
     3541 taaatcggat ccggcggcga ccagacgtcc agtttcttct ccaggcccat ggcggccaat
     3601 gtctgggaag atactttgtc attgaccgtc tttactgtaa gtgtcttgga aaagtacttt
     3661 gcccggttgg gaagtactac gctcaccggc cagcccttct ccgtaaggaa gcacgccagc
     3721 gtttcatgat aaacaccggt ggcttccaac acaaactgac agggcatgga tggatccatc
     3781 agcttccgga tccatttgga gagcttgacg aagcctcccg ggccattggg gatggtcagc
     3841 gtcgatacat actccacccg aaggtcggca tacatgcggc tgaagctggc aacatgctcc
     3901 ttcatcccgc aatcaattcc aatgcattgg cgaataacgt tctccatgac tgaatgggtt
     3961 ttgatctcgg atgataagca tactccccct cgtctttact cgtaacctac tcaagctgtc
     4021 tctgacatcc aggacgcctt ggattctgtt cagactctaa agagtaagaa gatgacgggg
     4081 gcatatctct gtgagagcat gtctcacgga ctgcttgggc acggatcacc tcccgccacc
     4141 ttacttatca accttgcctc taatttccta cttttaagga atggcgctaa cgtacgaggc
     4201 acacggagtt ttttaaggct gagccctgtc tgatcagagg ccctgagaag cccgaaatag
     4261 c
//
LOCUS       DAOTON010000131         4261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16825_length_4261_cov_3.598906, whole genome shotgun sequence.
ACCESSION   DAOTON010000131 DAOTON010000000
VERSION     DAOTON010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16825_length_4261_cov_3.598906"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="P5292_03750"
     CDS             complement(<1..1402)
                     /locus_tag="P5292_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38272.1"
                     /translation="MAKTASPDLFLLIRSLSKHEKGYIRTYAFGKRQDDPKFMRLFTA
                     IERLREYDETALLKSETYIRQLPRMKMYLYERILDALDNYYSVRNAQLEVRKVLNRIT
                     ILFEKGLYDACIRQLPKAKELASRYEFFGQWMEAISWERTLMLEKQLVENFAKVEAEE
                     RLVITRLEELAACNVVYDQINKLYSESIWIRSEADHRKYKTALNAPVMRRPDAGMGVL
                     ARVLRYKARAKYYSALDERKRYLEVSRRAVELFDSDPAFTNQHLLAYIKALNNLIATL
                     GENDRFQEFDRALEKLRSIPDRFPAGNSEKMRSMIRMRVLLREFYQNLYLREYDKAGE
                     LTETIEERLQKDRHLISAVHAVIFQYALSYIHFINGDYRKSLRWINAILNLKPLPVNR
                     LFQGYARVLGIILHFELGDVDMLQSLVPSALRFFDKCPDVYPLERMFIHQLWRKVVRT
                     SSDMERKKRLQELGSMI"
     gene            1612..3447
                     /locus_tag="P5292_03755"
     CDS             1612..3447
                     /locus_tag="P5292_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38273.1"
                     /translation="MRKPILLLSFLVLIAVGQLRAQVFSNTTSVPIPSGGTELRIPVT
                     VMGLPTVLNSSFGLGALCFDITHQADVNLRIQLESPDGHRITLIDQKGSNGDNFTGTC
                     LMENGVNGWVMTGTAPFTGSYFPMQSLNLLNNGQDPNGVWYFCVTDLFLPADTGSFHY
                     VNLTFMNNPPADPTGTSGPCNEQNAGACQCPTPGSTDCDLLPDVTASQLSMQQDHHEY
                     NGHITVGVGTPNIGWGPLEIHGIQSCYCDTVQVSCSTSFCPSGEPPKQLVNQRIYHKT
                     GNNMTYFDRPAGTMSYHPSHGHIHVDNWLYYTLRTWSPDPDPSNWPIVGTGTKMSFCL
                     INLSDCDSWYGYCVDSTGRVLTRADFPNADFGNVSGCTNDQGIYVGNIDEYSSGMNGA
                     GIVIPNACNDTYYIVAITDPLNLFLESNERNNWAVLPITLNMQTNANLLPSGFTYTLN
                     GLSINSESNASGADTCIWVWGDGTRDTVLANSVAHHSYTAPGSFVLFHYAINQCGPTV
                     SADTIQVLSTGLGSPVELAETGLFPNPSKDQTFLIYSLASSSQPVIEVFDALGNRLKQ
                     IQPGTQFPGKYRVPMSSASENWTAGTYFIRIRTANSEKTLRWIVF"
     gene            3598..>4261
                     /locus_tag="P5292_03760"
     CDS             3598..>4261
                     /locus_tag="P5292_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38274.1"
                     /translation="MKRGVLITFLLFLGWIASIATPPSNHFRSLPKYRKHRIGLGIEG
                     GGSFLLPDQPAGLAAVLEAYYCKDLGEHLLAESGAGVRLTSLHTRFELDGANHEDSTA
                     SINSLSIPLRLRYVISRWNVAPTVGLSYECRISTGISIDSVEQGKQGAWLKQARYNSN
                     TGVAAGNLDMQLGVSYMSPNRRTALGFLFNRNLLDWASDGYPEARAWGLSLRIDVTFG
                     LDP"
BASE COUNT          987 a         1142 c         1079 g         1053 t
ORIGIN      
        1 ggatcatact acccagttcc tgcaatcgtt tcttcctttc catatcagaa gatgtccgaa
       61 ccacctttcg ccaaagttgg tgtataaaca ttcgttcaag cggatagacg tcagggcact
      121 tgtcgaagaa ccggagtgca gatggaacca gtgattgcaa catgtcaacg tcgcccagtt
      181 cgaagtgaag gataatacca agcacgcggg catatccctg aaagaggcga tttaccggga
      241 gcggcttaag attgagtatg gcattgatcc aacgcaggct tttgcggtag tcaccgttga
      301 tgaagtggat gtaggacaat gcatactgga agatcaccgc gtgcacggcg ctgatcaggt
      361 ggcggtcctt ttgtaaacgc tcttcaatcg tctcggtcag ttctccggcc ttatcgtatt
      421 cacggagata caggttctgg taaaactcgc gcaggaggac acgcatcctg atcatggatc
      481 gcattttctc agaattaccg gcggggaaac ggtcgggaat gctcctgagt ttctccaaag
      541 cccggtcgaa ttcctgaaaa cgatcgttct caccgagtgt tgcgatcagg ttgttcagcg
      601 ccttgatata cgcaagcaaa tgctggttgg tgaaggccgg atccgaatca aacaattcga
      661 cggcgcgtct tgatacttcc aggtatcgtt tccgttcgtc cagtgcgctg taatactttg
      721 cgcgcgcttt gtagcgcaac acgcgcgcaa gcacacccat gcccgcatcg gggcggcgca
      781 ttacgggcgc gttgagtgcg gtcttgtatt tacggtggtc cgcttcactt ctgatccaga
      841 tcgattcgct gtacagttta ttgatctgat cgtacacgac attgcaggct gcaagctctt
      901 ccaggcgggt gattaccaat cgctcttccg cctccacctt cgcaaaattc tctacgagtt
      961 gcttctcgag catcagcgtt cgctcccagg agatcgcttc catccattga ccgaaaaact
     1021 cgtagcgact ggccagctcc ttcgctttcg gtagttgtcg aatgcaggcg tcatataatc
     1081 ctttttcgaa taagatggta atacgattca ggaccttcct gacttccaac tgagcgttcc
     1141 gcaccgagta ataattatcg agggcatcga gtatgcgctc gtacaagtac atcttcatcc
     1201 ttggcagctg ccggatatag gtttcggatt ttagtaaggc agtttcatcg tattcccgta
     1261 gccgctcaat cgccgtaaac agtcgcatga acttgggatc gtcctgccgc ttaccgaaag
     1321 catatgttcg gatgtatccc ttttcgtgct tactgagcga acggataagg agaaaaagat
     1381 ccggtgatgc cgttttggcc atgggtcaaa gatagatcac ctgcgacatt cagcacacgg
     1441 aatggcgggt cgggtttatt cgacaattcg atttcaggcc caaaagcaaa acgaattctg
     1501 ctcgttgacg gtgattaaga ctgcgtcgga ttgaatttta tccgggattt tgattcggct
     1561 tgcccgggtt gattccggaa ttttacggta caccaattca atttacccct catgcgaaaa
     1621 ccaatcctcc ttctgtcatt cctggtactg atcgcggtcg gtcaactgcg agcgcaggtt
     1681 ttcagcaaca ccacctccgt gccgattccc tccggcggaa cagaactgcg gataccggta
     1741 accgtcatgg gccttccaac ggtactgaac agttccttcg gtctcggcgc cctgtgcttt
     1801 gacattacgc accaggccga tgtcaatctc cgcattcaac tcgaatctcc ggatggtcac
     1861 cgcatcacgc tgattgacca gaaaggttcg aatggagaca acttcaccgg aacctgtctt
     1921 atggaaaacg gcgtgaacgg ctgggtcatg accggcactg cgccgtttac cggaagttac
     1981 tttccgatgc agtcgctcaa cctgctgaac aacggacagg atcctaacgg agtctggtac
     2041 ttctgcgtga ccgatctgtt ccttccggcc gataccggca gctttcatta tgtgaatctt
     2101 acgttcatga ataatccacc agccgatccg accggaacat cgggaccttg taatgaacag
     2161 aatgccggcg cgtgtcagtg cccaaccccc ggctcgacgg actgcgacct gcttccggac
     2221 gttacggctt ctcaactgag catgcagcag gatcatcatg agtacaacgg acatattacc
     2281 gtaggagtcg gcacgccgaa tataggatgg gggccgttgg agatccacgg cattcaaagt
     2341 tgttattgtg atacggtgca agtgagttgc tccacgtctt tttgtccaag cggggaaccg
     2401 cccaagcaat tggtcaatca gcgcatctac cacaaaacag gtaacaacat gacgtatttc
     2461 gatcgccctg ccggcaccat gagttaccat ccatctcacg gacacattca tgtcgataac
     2521 tggctgtact acacgctgcg cacctggtct ccggatcctg atccgagtaa ctggccgatc
     2581 gtcgggaccg ggacgaaaat gagtttttgt ctgatcaatc tgagtgattg cgactcctgg
     2641 tatggatact gtgtcgattc gaccggacgc gttctaaccc gggccgattt tccaaacgcg
     2701 gacttcggca atgtatccgg ttgtacaaac gaccagggca tctacgtcgg caacatcgat
     2761 gaatacagca gcggcatgaa tggagccgga atcgttattc cgaacgcctg caacgacact
     2821 tattacattg tggcgatcac cgacccgttg aatctttttc ttgaatcgaa cgaacggaac
     2881 aactgggcgg tcttaccgat tacgctgaac atgcaaacca acgcgaactt gttgccgagt
     2941 ggattcacct atacgctgaa cggcttatcc atcaacagcg aatccaatgc ctccggtgcc
     3001 gacacttgca tctgggtatg gggagatggt acacgcgata ccgttctggc aaactccgtc
     3061 gcccatcatt cctacaccgc accgggttcc tttgtgctct tccattatgc catcaatcaa
     3121 tgcggaccta cagtcagcgc tgataccatt caagtattgt cgaccggact tggcagtccg
     3181 gtcgaacttg cagaaaccgg attgtttccg aacccgtcga aagaccaaac gttcctgatt
     3241 tattcgttgg ccagttccag ccaaccggtc atcgaagtgt tcgacgcgct gggtaaccgg
     3301 ctgaaacaaa tccaacccgg gacccagttc ccgggtaaat accgcgtacc catgagcagc
     3361 gccagtgaga actggacagc cggaacttat ttcatccgaa tccggacggc caattcggaa
     3421 aagacgctgc gctggatcgt cttctagagc ggtttgtacg ttaacctgat catgttggtg
     3481 gtaacttgcc caggtgataa aacccctgtc cggatggaca ggggtttttt attcagtaaa
     3541 tacaatcgcg ttgatatccg tcattggact cgtttaaaag tatgttgacc tttaaacatg
     3601 aaaaggggag tgcttatcac attcttgtta tttcttggct ggatagcgtc cattgccacc
     3661 ccaccctcca accattttcg ctccctaccg aaatacagga aacaccggat cggcctgggg
     3721 atcgagggcg gtggatcttt tctgctgccg gatcaacctg ccggtcttgc ggcggtctta
     3781 gaggcctatt attgcaagga cctcggagag cacctgcttg cggagtctgg cgccggagtt
     3841 cggcttactt cgttacatac acgattcgaa ctcgacgggg cgaatcacga agacagtacc
     3901 gcttcgatca actccctctc cattcccctg aggttgcggt atgtcatttc cagatggaac
     3961 gtcgcgccga ccgtcggcct gagttacgag tgccggattt ccaccgggat ttcgattgat
     4021 tccgttgagc aggggaaaca aggagcctgg ttgaaacaag cgcgatacaa ttccaatacc
     4081 ggtgtagcgg ccggcaacct ggacatgcaa ctgggggttt cctacatgag tcccaaccgc
     4141 aggaccgcct tgggtttctt gttcaacagg aatttgcttg attgggcaag cgacggctac
     4201 ccggaagctc gtgcatgggg actttcgctc cggatagatg ttaccttcgg ccttgatcca
     4261 t
//
LOCUS       DAOTON010000132         4247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_16902_length_4247_cov_3.420086, whole genome shotgun sequence.
ACCESSION   DAOTON010000132 DAOTON010000000
VERSION     DAOTON010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16902_length_4247_cov_3.420086"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="P5292_03765"
     CDS             <1..1345
                     /locus_tag="P5292_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38275.1"
                     /translation="TFREWKDHWKELDWTRKWFPIFLFLRPVADNFYFLKDYGSLYSP
                     IYILGVLTPILCFVAVNRMKNIRTVAADQYMRIWAIMILVNGIALLTYDASMDNFGNT
                     LKYLSPPLLFFYLRHFIQSKRDLVFILQTFLYSAVYPYLSMALELVFGPVDPEYVSAG
                     RGGGARLRGYYADSANYFIYFINSVIISVYFFLDRIFTKSKTNPMTASKLLILFAAAL
                     IGIIQLRHVTTWGALAVIIALLLYFNSKNVKGYGVLIVVGLIVVPLLATLVYDQYIYP
                     LIAKEFNVWEGDVDIQYGFNGRISRWERYFEIWGQMPSIAHFFGVALSGFRQAPIMMS
                     GGMHNDFVRILFMTGIFGVALYVLFILSIFFRWTIYKVPERFLILTSVAALMMYSMTT
                     LPMIYPGFQYVFFSVFSFALLKKQKAYGAPPPRPVFRPSPQFPAGGPAPSTSTAS"
     gene            1345..2445
                     /locus_tag="P5292_03770"
     CDS             1345..2445
                     /locus_tag="P5292_03770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS38276.1"
                     /translation="MEKPVVVILGKIPPPYMGPSLATQIILKSSLNEQFRLVHVDTRA
                     VNSLTAMGKFSFAKIGRNLGIYRHLWKTIRREHPALVLVPISQSTIGFLKDAVFILIA
                     LLLGRKVLIHLRGSNFLNWMKGSSAITRTFVKSVLRRTNGVIVLGNNLRYLFAGIYPP
                     ERIYVVPNGADYALSSRPKSNHPVRLLYLANLQASKGIEDLVEAIRLLKEQGVADFRL
                     DVVGGWRSSATQQSCESLVQTHQLPVSFHPADAGEKKFQFLSEADIFIFPPRAPEGHP
                     WVVVEAMAAGLPIISTDQGAIVESVLDGKNGFIVPVRDPAAIAAHLKELIQNDAKRVE
                     MGQFSRHLYETEFTEKRMVERLAATFRAVIAA"
     gene            2457..>4247
                     /locus_tag="P5292_03775"
     CDS             2457..>4247
                     /locus_tag="P5292_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HRS38277.1"
                     /translation="MQQLTQNLKDGTMQLLEVPFPALTSGTVLVRNHFSLISAGTEGK
                     SVKDARLGYIAKARARKEEVKKVLQAVKTFGLKDTYRMVMNRLDAPSALGYSCAGEVI
                     AVASDVRDFQVGDRVACGGSTANHAEVVMVPVNLCVKLDPNVSLEQAAYTTVGSIALQ
                     GIRQADLRLGENCVVIGLGLLGQITIQLLRAAGVRTIGIDIDERMVRLAETMGCDLAL
                     SRRLEQLEDIVANFTGGHGTDAVIITAGTDSTDPVDLAGALCRKKGRVVVVGAVPTGF
                     KRPNYFKKELELRMSCSYGPGRYDPEYEEQGLDYPYAWVRWTENRNMMAFVELLRSRR
                     IDLEPLTTHRFDFNEAKNAYQLILDRAEPFVGVLLRYDTSRPLKTRIEFQERKKAGSE
                     PGIGLVGAGSFGQNFLLPALKGLGRMIHVSTARSNNARNIADKHGFVRCTGDASEVIN
                     DPEVNVLFIATRHDTHAALVLDGLKAGKDVFVEKPLCLHPDELTAIQAAYQQGQSRLM
                     VGFNRRFAPMIKKIKRTLSSNAPVAIQYRINAGHVAADHWIHNPSVGGGRIVGEVCHF
                     VDLCSFLAASQVTQVSAMTMADPANQQDTLS"
BASE COUNT          929 a         1166 c         1155 g          997 t
ORIGIN      
        1 gacatttcgc gagtggaaag atcactggaa ggaactggat tggacccgga aatggttccc
       61 gatcttcctg ttcctgcgtc ccgtcgccga caacttctac tttctcaagg actacggctc
      121 cctttattcg cccatctata tacttggtgt attgacgccg atcctgtgct tcgtggcggt
      181 gaaccggatg aaaaatatcc gtaccgtagc agcagaccag tatatgcgga tctgggccat
      241 catgatcctg gtaaacggga tcgccttgct cacctacgac gcttctatgg ataacttcgg
      301 caacacgctg aagtacttat cgccacccct gttgttcttt tatctccgcc actttatcca
      361 gtcaaagcgc gacctcgttt tcattctcca gacgtttctc tactcagctg tctatccgta
      421 cctctcgatg gccctcgaac tggtcttcgg tccggtcgat ccggaatacg tctcggctgg
      481 tcggggcgga ggcgcccgtc tgcgggggta ttatgccgac agcgccaatt attttatcta
      541 cttcatcaac tcggtaatca tcagcgtcta tttcttcctg gatcggatct tcaccaagag
      601 caagacgaat ccgatgaccg ccagcaaact gctcatcttg ttcgcggcgg cgctgatcgg
      661 tatcatccag ttgcgacacg tgactacctg gggagccctg gcggtgatca tcgcactgct
      721 gctttacttc aattcaaaaa atgtcaaggg gtatggcgtg ttgattgtag tgggattgat
      781 cgtggtaccc ttactggcaa cactcgttta cgatcagtac atctatcccc tgatcgcaaa
      841 ggaattcaac gtgtgggaag gggatgtgga catacaatac ggtttcaatg gtcggatatc
      901 ccgttgggag cggtactttg agatctgggg tcagatgcca tccatcgctc acttctttgg
      961 cgttgcattg tccggattcc ggcaggcgcc gatcatgatg agtggaggta tgcacaatga
     1021 ttttgtccgc attcttttca tgaccggaat tttcggtgta gcgctttatg tattgtttat
     1081 cctcagtatt ttctttcgct ggaccatcta taaagtgccc gaacgtttcc tcatcctgac
     1141 ctcggtggcg gccctgatga tgtattccat gaccacgctc ccgatgatct atcccgggtt
     1201 ccagtatgtg ttcttttcgg tgttcagttt cgcattgctg aagaagcaaa aagcctacgg
     1261 ggcgccgcct ccccgaccgg tgtttcgtcc ttcgcctcaa ttcccggcag ggggaccggc
     1321 cccatcaact tcgaccgctt cctgatggaa aaaccggttg ttgtcattct gggtaagatc
     1381 ccgccgcctt acatggggcc ttcactcgcc acgcagatca tcctgaagtc ctccctgaat
     1441 gaacagttca ggctggtaca cgtcgatacg cgggcggtaa actcgctgac cgcgatgggt
     1501 aagttttcgt ttgcgaaaat cggacgaaac ctcgggatct accgtcatct ctggaaaacg
     1561 atccggcgtg agcacccggc tttggtgttg gtacccatca gtcagtcgac gatcggcttc
     1621 ttaaaagacg cggtctttat cctgatcgcc ttgctccttg gtcgcaaagt gctcatccac
     1681 ctgcgtggta gcaatttcct caactggatg aagggtagta gcgcgatcac acggacgttc
     1741 gtcaaaagtg tcctgcgacg aaccaacggt gtcatcgtac tcgggaacaa cctgcgctac
     1801 ctgttcgcgg ggatctatcc gcctgaacga atctatgtgg tccccaacgg tgcggattat
     1861 gcactcagca gccgacccaa atcaaatcat ccggttcgcc tgctctacct ggccaacctg
     1921 caggcatcca aagggatcga agacctcgta gaagcaatcc ggttactgaa agagcaggga
     1981 gtggcggatt ttcgactgga tgtggtgggc ggttggcgat catcggcgac ccaacaatct
     2041 tgcgaatcac tcgtgcagac gcatcaactg ccggtcagct ttcatccggc cgatgccggt
     2101 gagaagaaat ttcaattctt atccgaagcc gatatattca tctttccgcc gagagcgccg
     2161 gaaggtcacc cctgggtggt ggttgaagcc atggctgccg gactgccgat catctccacc
     2221 gaccagggag ccatcgtcga gagtgtgttg gatggaaaga atggattcat tgtacccgtt
     2281 cgtgatccgg ccgctattgc agcgcacctg aaggaactga tccaaaacga cgcgaaaaga
     2341 gttgaaatgg gacagttttc gagacatttg tatgaaacgg aatttaccga aaaacgtatg
     2401 gtagaaagac tggctgcgac ctttcgggcc gttatcgccg cgtagtacca catcgtatgc
     2461 aacaactgac gcagaatctg aaagacggaa ccatgcaact gctggaagtt ccttttccgg
     2521 cattgacatc cggtacggtt ctggtcagaa atcatttttc cctgatcagc gcgggtacgg
     2581 aaggtaagtc ggtcaaggac gcgcgcctgg ggtacatcgc caaagcacgt gctcgtaagg
     2641 aagaagtcaa aaaggtattg caagcggtca agacgttcgg actgaaagac acctaccgca
     2701 tggtcatgaa tcggctcgat gcgccctcag cgctcggcta tagctgcgcg ggggaggtga
     2761 tcgcggttgc atccgatgtc cgtgatttcc aggtcggcga tcgcgtagcg tgcggcggtt
     2821 ctaccgccaa tcacgcggag gtggtgatgg ttcctgtcaa cctctgcgtg aagcttgatc
     2881 cgaacgtatc actggaacag gcggcctata cgaccgtcgg ttccatcgcc cttcagggga
     2941 ttcgccaggc ggatctgcgg ttgggtgaaa attgcgttgt gatcggactc ggactcctgg
     3001 ggcagatcac cattcagttg ctgcgtgcag caggggtacg aaccatcggg atcgacatcg
     3061 acgagcggat ggtccgcctc gcggaaacga tgggatgtga cctcgccttg tcacggcgcc
     3121 tcgaacagtt ggaagacatc gtggctaact ttacgggcgg acacggtacc gacgcggtaa
     3181 tcatcacagc cggcaccgac tccaccgatc cggtcgatct ggccggcgca ctttgcagga
     3241 agaagggcag ggtagtggtt gtcggagcgg tcccgaccgg attcaagcgt ccgaattatt
     3301 tcaagaaaga actggaactc cgcatgtcct gctcctacgg tcccggtcgt tacgatccgg
     3361 aatacgaaga acagggactg gattacccct atgcctgggt acgctggaca gagaaccgga
     3421 atatgatggc ctttgtcgaa ctgctgcgtt caagacgcat cgacctggag ccactgacga
     3481 cgcaccgctt cgatttcaac gaagcgaaaa acgcttacca actgattctc gaccgcgccg
     3541 aaccctttgt cggggttctc ctgcgatacg atacgagccg tccgttaaag acccgcatcg
     3601 agtttcagga acgaaagaag gcgggaagcg aaccgggtat cggcctggtc ggcgcgggtt
     3661 ccttcggaca gaacttcctg ctgccggctt tgaaaggcct cggccggatg atccatgtct
     3721 ccaccgcccg gtcgaacaac gcgcgcaaca tcgccgataa gcatggcttc gtgcggtgta
     3781 ccggcgatgc ttccgaagtc atcaacgatc cggaagtcaa cgtccttttc attgcaacac
     3841 gacacgatac ccatgcagcc ttggtgctcg atggcttgaa ggccgggaag gatgtattcg
     3901 tcgagaagcc gctctgcctg caccccgacg agttgacggc tattcaagca gcctatcagc
     3961 aaggacaatc gcgtctgatg gtaggtttca accgtcgttt cgctccgatg atcaaaaaga
     4021 tcaagcgaac actctcatcg aacgcgccag ttgccatcca atacaggatc aatgcggggc
     4081 atgtggccgc tgatcactgg atccacaacc cctcggtagg aggtgggcgc atcgtcggag
     4141 aagtctgcca cttcgtggac ctctgttcat tcctggccgc gtcgcaggtt acgcaggttt
     4201 cagccatgac catggccgat cccgccaatc agcaggatac actttca
//
LOCUS       DAOTON010000133         4212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17065_length_4212_cov_3.299976, whole genome shotgun sequence.
ACCESSION   DAOTON010000133 DAOTON010000000
VERSION     DAOTON010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17065_length_4212_cov_3.299976"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="P5292_03780"
     CDS             <1..384
                     /locus_tag="P5292_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRS38278.1"
                     /translation="SQFSYQTSAIPPVRALYFIWREPWMAAGNNTFIHSMLRQAGFLN
                     VAGHLERYPELPLAAWSDLQPEVVMLSSEPYPFAEKHMAAVQSAFPDSKVTLVDGEMF
                     SWYGSNLLLAPDYFGKLTRVIAAMD"
     gene            407..2686
                     /locus_tag="P5292_03785"
     CDS             407..2686
                     /locus_tag="P5292_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRS38279.1"
                     /translation="MKFRLILLFYLALTVSLHAQKVGLVLSGGGARGMAHIGAIRALE
                     ESGIPIDYIAGTSAGAIVGCMYALGYSPHEMDSIVRTDDFYNWANGIIDENYFYYYRK
                     NPENASWVNLKFSLDSLLQTSLPTNLVSSVPYDFALLEQTAAASAATRYDFDSLFVPF
                     RCVAADIENKQTIIFREGDLGQAVRASSAYPFYFRPMVFQNKILYDGGLYNNFPADVM
                     LEDFQPDIIIGVNASGKITPTTEGNIISQIRAMMTTPTEFSVICENGILVEAKTDEFG
                     LFDFSDLPSIIQEGYRATMEKIDTIRFNVHRRINADQVHRQRKVFRSRFPEVVINNVS
                     IDGVNSKQSEYVHRILKAGSQPIPLSRLKASYFQLVSDPNIRAIQPKLMFDPSTGMFD
                     LNVRIQQERDLITQFGGLLSSRPISEVFAGLTYNIWSRRAWSFNTNFYFGKLYTSGQA
                     RIRMDAPFRKPFFLEADATLNQYDFFRSSNAFFSDQKPAYILASDYFFGLHGGIPARN
                     KGKIVASAGYVRIADNYYQTQNFLQKDTADKTILKGFTGALTFERSTLNRKQFANQGT
                     FLQLRIRGVDLEEDTKPGSTSASRTIEHDHHQWLQAKLTYENYYKRRGRLRLGFYSEV
                     AATNQTFFNNYTATILNTPSFEPVLETQTLFLPAYRAQFFGGAGLRNVIMLRNNLELR
                     IDGYVFQPYQELIRTDDLKTRFGEPLAKRYFLGSGGLVLHSPIGPISLAVNYYDQREE
                     NLSLLFHVGFLLFHASALD"
     gene            2726..3244
                     /locus_tag="P5292_03790"
     CDS             2726..3244
                     /locus_tag="P5292_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRS38280.1"
                     /translation="MKTLLLILTMMLPSAPGSLYDFTANGIDGKPVDFSAFKGKKVLI
                     VNTASQCGYTPQYAQLEELAKANKGKLVVIGFPANEFGGQEPGSNTEIRDFCSKNYGV
                     TFQMMEKVVVKGESIHPLFQWLTTEANPDFTGDINWNFEKFLLDEQGKLIHRYRSKVN
                     PMGDEIRAALGQ"
     gene            3301..3660
                     /locus_tag="P5292_03795"
     CDS             3301..3660
                     /locus_tag="P5292_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38281.1"
                     /translation="MHSNAQIRKYENFHIFLWLIKDLCWVTVSRLAGMFMILPTLSLA
                     IYITWKNRAERAELFHNLAVCFWISANSIWMYGEFYLDDTTRHPAVIFFVAGLLSMGY
                     YYLIEILGTSRSKVRKE"
     gene            complement(3666..3971)
                     /locus_tag="P5292_03800"
     CDS             complement(3666..3971)
                     /locus_tag="P5292_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRS38282.1"
                     /translation="MEDQQNQLNIELSEEIAEGIYSNLAIITHSNSEFVLDFVKVMPG
                     VPKAKVKSRILLTPQHAKRLLAAMQDNIAKYEAVHGPIKQTEGFGMPMNFGGPAAQA"
BASE COUNT         1015 a         1122 c         1015 g         1060 t
ORIGIN      
        1 agtcagtttt cctatcaaac atcagcgatt ccaccggttc gtgcgcttta tttcatctgg
       61 cgggagccct ggatggcagc aggcaacaac accttcatcc attccatgct tcggcaagcc
      121 gggtttctca atgtcgcagg ccatctggag cgatatcctg aactgccctt agcggcgtgg
      181 tccgaccttc agcccgaggt ggtcatgctg tctagcgaac cctacccctt cgcggaaaaa
      241 cacatggccg ctgttcaaag cgcctttccc gattcaaaag ttacgctggt tgacggcgaa
      301 atgttctcct ggtacggctc caacctgctg ctggcacccg attattttgg aaaattaacc
      361 cgtgtcatcg cggctatgga ttaattttgt cttccccgct ccgcacatga aattccggct
      421 gatcctcctt ttctacctgg ccttgactgt ttcgcttcat gcgcaaaaag tcggccttgt
      481 actgagtggc ggcggtgcgc ggggtatggc acacatcggt gcaattcgtg cgttggagga
      541 atcgggaatt ccgattgact acatcgcggg gacttcggca ggcgccattg tcggatgcat
      601 gtatgcgctc ggttactcgc cgcacgaaat ggattccatt gttcgcacgg atgatttcta
      661 caattgggcg aacggcatca tcgacgagaa ctacttctac tattaccgga aaaatccgga
      721 gaatgcctct tgggtcaacc tgaagttttc gctggactca ctgcttcaaa caagtctccc
      781 gaccaacctg gtcagttcag tgccatacga tttcgcactg ctggaacaaa cagcggcggc
      841 ctcagcggct acccgctatg atttcgattc gctcttcgtc ccctttcgtt gtgttgcagc
      901 ggacatcgag aacaaacaaa ccatcatctt ccgtgaaggt gacctcggtc aggcggtcag
      961 agcatcttcc gcctatccat tttacttccg cccaatggtc tttcaaaata agatcctgta
     1021 cgatggcggg ctctacaaca acttcccggc ggatgtgatg ttggaggatt ttcaacccga
     1081 tatcatcatc ggggtgaatg catccggaaa gatcacgcct accacggaag ggaatatcat
     1141 ttcccagata agggcgatga tgacgacgcc gacagaattc tccgtcatct gcgaaaacgg
     1201 cattctggtt gaagcgaaaa ccgacgaatt cggtctattc gatttttcgg atctgcccag
     1261 tatcatccag gaaggctacc gggcgacgat ggagaagatc gatacgatac gtttcaacgt
     1321 acaccggagg atcaatgcgg accaggtcca tcgacagcgt aaagtatttc gttccaggtt
     1381 tccggaagtg gtcatcaata atgtctccat cgatggcgtc aacagcaagc aatcggagta
     1441 cgtacacagg atactgaagg cgggctctca gccgattcca ttatcccgcc tgaaggcatc
     1501 ctattttcaa ttggtctcgg acccgaatat ccgtgctatt caacccaagc tgatgttcga
     1561 tccgtcaacg gggatgttcg atctaaatgt gaggatccag caggagcggg atctgatcac
     1621 gcaattcggc gggctgctct catcgcgacc gatttccgaa gtattcgcgg gattgaccta
     1681 caacatctgg agccgaagag cctggagttt caacaccaac ttttatttcg gcaaactata
     1741 cacctccggc caggcaagga tcaggatgga tgccccattc cggaagccgt tttttctgga
     1801 agctgacgcg acactcaacc agtacgattt cttccggagc agcaacgcat ttttcagtga
     1861 ccagaagcct gcctatatcc ttgcgtctga ttactttttc ggcctgcacg gtggaattcc
     1921 cgcacgaaac aagggtaaaa tcgtcgcgag cgccggctat gtacgcatcg cggacaatta
     1981 ttatcagacg cagaatttcc tccagaagga cacggctgac aagaccattc tcaagggctt
     2041 cacgggcgcg ttgacctttg aacggagcac cctgaacaga aaacagtttg ccaaccaggg
     2101 cacattttta cagttgcgca ttcgcggtgt tgacctggaa gaagacacaa agcctggttc
     2161 cacttccgct tcgagaacga tcgagcacga tcatcaccaa tggctgcagg caaaactgac
     2221 ctatgaaaat tactataaac ggcgcggacg tcttcgtttg ggcttctatt cggaagttgc
     2281 agcgaccaac cagacctttt tcaacaacta caccgctacg atcctgaaca cgccttcctt
     2341 tgagccggta ctggaaaccc aaaccttgtt tctacctgcc taccgtgctc aatttttcgg
     2401 cggagccggg cttcgaaacg tcattatgct ccggaataac ctggaattgc gcatcgacgg
     2461 ctatgtattt caaccttatc aggaactcat ccgcacggac gatctgaaaa cccgctttgg
     2521 cgaaccgctg gccaaacgtt atttcctggg atccggtggc ctggttttac attcaccgat
     2581 cggcccgatc agtctggcag tgaattacta tgatcaacgg gaagagaatc tgtcactctt
     2641 gtttcatgtc ggattcctgc tctttcatgc ttccgccctt gactgaacct ttcgccgaat
     2701 ccattgttac tgcagatata caactatgaa aacactcctc cttatcctca ctatgatgct
     2761 accgtctgca cccggctccc tctatgactt tacagcaaac ggcatcgatg gtaagccggt
     2821 cgatttttcc gcattcaaag gcaagaaagt actgatcgtg aacaccgctt cccaatgcgg
     2881 ttacacacct caatacgctc aactggaaga attagcgaag gccaacaagg gtaaactggt
     2941 cgtgatcggc tttccggcca acgagttcgg cggtcaggag cccggcagca atacggagat
     3001 ccgggatttt tgcagtaaaa actatggggt cacttttcaa atgatggaaa aggtagtggt
     3061 gaaaggcgaa tccattcatc ccttgtttca atggcttacc acagaggcaa atccggattt
     3121 tacgggtgat atcaactgga atttcgaaaa gttcctcttg gatgaacagg gcaaattgat
     3181 tcaccggtac cgcagcaagg tcaacccgat gggtgatgaa atacgtgctg cattaggaca
     3241 ataaagcgaa tagtccgatc atcctcgaaa gttaatcgaa caatcgatta actttcggac
     3301 atgcactcca acgcccaaat tcgtaagtac gagaatttcc acatcttcct ctggctgatc
     3361 aaagacctgt gttgggttac cgtttcccga cttgcgggca tgttcatgat cctgccgacc
     3421 ctgagtctgg cgatctatat tacctggaaa aatcgcgcgg aaagagcgga gctattccac
     3481 aacctggcgg tttgcttttg gatatccgcc aattccatct ggatgtatgg cgagttttac
     3541 ctggatgaca caacccgaca tccggcggtt attttcttcg tcgccggatt gctgtcgatg
     3601 ggctattatt acctgatcga gatactgggg acttcacgta gcaaagtccg gaaagagtaa
     3661 agcacttaag cttgcgcggc cggcccaccg aaattcatcg gcatcccgaa gccttccgtt
     3721 tgtttgatcg ggccgtgaac agcttcatac ttggcgatat tatcctgcat ggctgccagg
     3781 agtcgcttgg catgctgcgg agtcagcaga atacgcgatt tcacctttgc cttaggaacg
     3841 cccggcatca cttttacaaa atccagaacg aattctgaat tgctgtgcgt aatgatcgcg
     3901 agattggaat atatgccttc tgcgatctct tccgataact cgatgttgag ttggttttgc
     3961 tggtcttcca ttgtatggcg cttgtacgta tgatacaaat atcctaaaaa accgtcgttc
     4021 cccttcaaaa aaaacgcccc gggtcttccg gggcgtttcg ttcaattatt cgtcaggaat
     4081 cagcgggtac cgacttcttc ctcgtccttg gatgccatga ggagatcata ctcctcctgg
     4141 gaaccgacga taagtttatc gtactcgcgc aatccggtac cggccgggat gaggtggccg
     4201 acgatcacgt tt
//
LOCUS       DAOTON010000134         4196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17147_length_4196_cov_2.646704, whole genome shotgun sequence.
ACCESSION   DAOTON010000134 DAOTON010000000
VERSION     DAOTON010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17147_length_4196_cov_2.646704"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="P5292_03805"
     CDS             complement(<1..417)
                     /locus_tag="P5292_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRS38283.1"
                     /translation="MQRFFTLLCCLFFLFINANAQQTFPVNGPTDPRHITYAITNVRL
                     YVDYKTVVDPATILIRDGLILEAGAGVTVPSDAVVYDYKGKTIYPSLIELVSDYGQPE
                     LKRPQGNFEDPQLLTNTKGAYDWNQAVKAEVDAYRSF"
     gene            complement(491..1669)
                     /locus_tag="P5292_03810"
     CDS             complement(491..1669)
                     /locus_tag="P5292_03810"
                     /inference="COORDINATES: protein motif:HMM:NF018880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38284.1"
                     /translation="MTRRLLFTLFGLMLTGFQGFAQPFAIGHLSTTYTDAARSRPVPV
                     EIYYPADQAGDGVPVAAGNGEAYPLLVFGHGFVMSWDAYQNIWEAVVPSGYIIAFPRT
                     ETGLSPQHAALGQDIAFVRSALLNENSDPSDLFYQRVATASAAMGHSMGGGASFLAAA
                     QDTGFRVLVNFAAAETTPSAITAAAGIDLPALLFAGENDCVTPIAAHQQPMYDALGSN
                     CRNLVTIRGGSHCQMAESNFYCNIGEASCTPAPAISRAVQHDCINRFLLPWLDAQLKA
                     DCAAGLRFDSLVLNDTSVSVQRTCAACVTTAITPPVKAGPSVTLSPSGDQLLFSGLNL
                     EAGDAIRLSDLSGRELTFHRLPAAANEFRLNLPVLPPGAILLRLERNSMVIWAGKVLR
                     "
     gene            1757..2410
                     /locus_tag="P5292_03815"
     CDS             1757..2410
                     /locus_tag="P5292_03815"
                     /inference="COORDINATES: protein motif:HMM:NF016161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRS38285.1"
                     /translation="MSSALLAKIHFISALLFLILYLVKLILLFTNKKALQSMTAVTKV
                     PEMIISTLFLVTGVWLLVILGGIKLFHIIKLVFVFLAIPLGVIGFKKQRYAAAILSVL
                     FIVLAYGMAEMSKNKPFLPAKVVMNNDDGSPLAQGAKVYHANCVFCHGTDGKKMYRTA
                     PDLSTSSLDALSMQGMITDGSKGKMPAYGKVLSAEEIAQVALYVQSLKTGSNIQVNP"
     gene            2423..3622
                     /gene="hflX"
                     /locus_tag="P5292_03820"
     CDS             2423..3622
                     /gene="hflX"
                     /locus_tag="P5292_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRS38286.1"
                     /translation="MSKHLHPTGRKQETAVLIGLLQKGQSDRQLTEYLDELAFLTETA
                     GALTLRRFSQKLDAPDSKTFIGSGKLNEIRDFIAEHKVDLAVFDDELSGTQIRNIEKV
                     LNCRVLDRSNLILDIFATRARSAQAKAQVELAQYQYLLPRLTRMWTHLEKQRGGIGMR
                     GPGESEIETDRRAIRDRLARLKEQLRAIEKQSATQRKQRGEFVRVALVGYTNVGKSTL
                     MNLLSKSDVFAENKLFATLDTTVRKVVYDRIPFLLSDTVGFIRKLPHGLIESFKSTLD
                     EVREADVLLHVVDISHPQFEDQIAVVKETLAELGTADKPTLMVFNKIDAYHYVPKEED
                     DLTPETKENLSLEEFKKSWMSQLGEQCVFISAANRTNIDELRLRLTSMVKEIHLVRYP
                     NYLGSEQ"
     gene            complement(3830..>4196)
                     /locus_tag="P5292_03825"
     CDS             complement(3830..>4196)
                     /locus_tag="P5292_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRS38287.1"
                     /translation="EGIAQTSQRKAVIDLSSGDTAVMSVTLRQLRNFRNADPQAQLEL
                     VCHGPAVKLLMTDSTFFREQVVSLKKEQVEFKACGNSMKRMNIKAEQLLPESDVVPAA
                     IVELSDKQQEGWSYIKAGR"
BASE COUNT          984 a         1157 c         1197 g          858 t
ORIGIN      
        1 aaagctgcga taggcatcca cctccgcctt gacggcctgg ttccagtcgt aggcgccctt
       61 ggtattcgtc agcaactgcg ggtcttcgaa atttccctgc ggacgcttca gttcgggttg
      121 accgtaatcg gaaaccagtt cgatcagtga aggatagatc gtctttcctt tgtagtcgta
      181 cacgaccgcg tcggatggaa ccgtgacacc ggcgccggcc tccaggatca atccatcgcg
      241 tatcaggatg gtggcgggat caaccacggt cttgtagtcc acgtagaggc ggacattcgt
      301 gatcgcatag gtgatgtgcc gggggtcggt gggaccgttg acaggaaagg tttgctgcgc
      361 gttggcgttg atgaacagaa agaacaggca gcacaggagc gtaaagaatc gttgcatgca
      421 ggtcgtttaa ggcgaccgaa gataacgatt cgtcgagtgt taaaaggtga atgaaacctt
      481 aaaatcggtg ttaacggagg accttcccgg cccaaataac catcgaatta cgttccaagc
      541 gcagcaggat ggctcccggt ggcagcaccg gcaggttgag ccgaaattca ttggccgccg
      601 ccggcaaccg atggaaggtc agctcccttc cggacaggtc cgacaacctg atagcatcac
      661 cagcttccag gttcaggccg ctgaacagca gttggtcgcc ggacggactc agtgttacgc
      721 tcggaccagc cttcacagga ggagtgattg ccgttgtgac acaggccgca catgtccgct
      781 gaaccgacac actggtatcg ttgagtacaa gcgaatcgaa acgcagaccg gccgcgcaat
      841 cggctttcag ttgcgcgtcg agccagggaa gcaggaaccg gttgatgcag tcgtgctgga
      901 ccgcgcggga aatcgcggga gccggagtgc aactggcctc accgatgttg caatagaaat
      961 tcgattccgc catctggcaa tgactgccgc cccggatggt cacgaggttg cggcagttgc
     1021 tgccaagcgc atcgtacatc ggttgttgat gtgccgcgat cggtgtcacg cagtcatttt
     1081 caccggcaaa caaaagtgcg ggaaggtcga tgccagccgc agcggtgatc gcggaaggcg
     1141 ttgtttcggc agcagcgaaa ttgacgagca cacggaaacc cgtatcctgt gcagcagcca
     1201 gaaagctggc tcctccgccc atcgaatgcc ccatggcggc ggaagccgtt gcgacgcgct
     1261 gatagaacaa gtcggaagga tcgctgtttt cattgagcag ggcggaacgc acaaaggcga
     1321 tgtcctgtcc gagtgcggca tgttggggag ataagccggt ctcggtgcgc ggaaacgcga
     1381 tgatgtagcc ggaaggaacg accgcttccc agatgttctg atacgcatcc caactcatca
     1441 cgaagccgtg tccgaatacc aacaaaggat acgcttcccc gttgcccgca gcgaccggaa
     1501 caccgtcgcc ggcctggtcc gcaggatagt agatctccac cggaaccggt ctgcttcggg
     1561 cagcatccgt ataggtggtg ctgagatgac cgatggcaaa gggctgggca aagccttgaa
     1621 agcctgtaag catcagtccg aaaagggtaa acagcagtcg cctggtcatt tccataggat
     1681 cggaaacaag ttaagccgat tttctgttca gaagaacacg cgtaaaaagc ctacttttga
     1741 cgaaaatcct ccccccatga gttccgccct actcgccaag atccacttta tcagcgccct
     1801 gctgttcctg atcctgtacc tggtcaagct gatcttactc ttcacgaaca agaaggcatt
     1861 gcagtcgatg accgccgtga cgaaagttcc ggaaatgatc atcagcacgc tctttctggt
     1921 gaccggcgta tggctcttgg tgatccttgg tggaatcaag ctgttccaca tcatcaaact
     1981 ggtcttcgtg ttcctcgcga tcccgctggg ggtgatcggc ttcaagaaac agcgctatgc
     2041 tgccgcgatc ctgtcggtgc tctttatcgt ccttgcgtac ggcatggcgg agatgagcaa
     2101 gaacaagccc tttctgccgg ccaaggtggt gatgaataac gacgacggca gtccgttggc
     2161 gcaaggcgcg aaagtgtatc atgccaactg cgtgttctgt catggcaccg acggcaagaa
     2221 aatgtaccgt accgcgccgg accttagcac aagcagcctc gacgccctca gcatgcaagg
     2281 catgatcacc gatggcagca agggtaaaat gcccgcctac ggcaaagtgc tctccgccga
     2341 agagatcgcg caggtcgcgc tctacgtgca atcgttgaag accggttcca acatccaggt
     2401 gaatccctga gcatagacat ccttgtccaa acaccttcac ccgaccggtc gtaagcagga
     2461 aactgcggtg ctcatcggcc tactgcagaa aggacaatcg gaccggcagt tgaccgaata
     2521 cctcgacgag ctggccttcc tgacggagac ggccggcgcc ctcacgttgc gtcgcttttc
     2581 ccaaaagctg gacgcacctg actccaagac cttcatcggc agcggcaagc tgaacgagat
     2641 ccgtgatttc atcgccgaac acaaggttga cctggcggtc ttcgacgatg aactgagcgg
     2701 cacacagatc cgcaacatcg aaaaggtgtt gaactgccgc gtgctcgacc gcagcaacct
     2761 catcctcgac atcttcgcca cgcgtgcccg cagcgcacag gccaaggcac aagtggagct
     2821 ggcgcaatac cagtaccttc tcccccgcct tacccgcatg tggacgcacc tcgagaagca
     2881 acgcggcggc atcgggatgc gcggtccggg cgaatcggaa attgaaacag accgtcgggc
     2941 catccgggac cgactggccc gcctgaagga acagttgcgc gcgatcgaaa agcaaagcgc
     3001 cacccagcgc aagcaacgcg gagaattcgt acgcgtagcc ctggtgggtt atacgaacgt
     3061 cgggaaatcc acgttgatga acctgctcag caaaagcgac gtctttgccg agaacaaatt
     3121 gttcgccaca ctggacacca ccgttcgcaa ggtggtatac gaccggatcc cgttcctgct
     3181 ctccgatacg gtgggattca tccggaagct gccgcacgga ctgatcgaat cgttcaaatc
     3241 aacgctcgac gaagttcggg aagcggacgt gttgttgcac gtggtcgaca tctcccatcc
     3301 gcagttcgag gaccagatcg ccgtggtaaa agaaaccctg gccgaactgg gtacggccga
     3361 caagcccacc ctgatggtgt tcaacaagat cgacgcctat cattatgtac cgaaggaaga
     3421 agacgatctg acgcccgaaa cgaaagagaa cctttcgctg gaagaattca aaaaaagctg
     3481 gatgagccag ttgggcgagc aatgtgtatt catctccgcc gccaatcgga ccaatatcga
     3541 cgagttgcga ctacgcctta cttcgatggt gaaggagata catttggtgc ggtatccgaa
     3601 ttatttgggg agtgaacagt gaaccctgcc cgccgtagct ttagcatagg cgggagtgaa
     3661 ccctgcccgc cgtagcttta gcgtaggcgg gagtgaacag cgaatagcga atagcgaata
     3721 gtaaaaggtg aacagttact agttactagt tactagttac taattactag ttcccgataa
     3781 taaaaaaaga aaccccgaca agcggggttt ctttttttgt tatcggggtt tatcggccgg
     3841 cttttatgta gctccagcct tcttgttgtt tgtcgctgag ttcgacgatg gcggcgggga
     3901 ccacgtcgga ttcgggtagc agttgctccg ctttgatgtt catgcgtttc atcgagttgc
     3961 cgcaggcttt gaactccacc tgttcctttt taaggctgac gacttgttca cgaaaaaaag
     4021 tgctgtcggt catgagcagt ttcaccgccg ggccatggca gacaagttcc agttgcgcct
     4081 gcgggtcggc attacggaag ttgcgaagtt gccgtagcgt gaccgacatc accgccgtgt
     4141 ctccgctgga gaggtcgatc accgctttgc gctgactggt ttgagcaatg ccctcg
//
LOCUS       DAOTON010000135         4195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17154_length_4195_cov_2.833092, whole genome shotgun sequence.
ACCESSION   DAOTON010000135 DAOTON010000000
VERSION     DAOTON010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17154_length_4195_cov_2.833092"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..442
                     /locus_tag="P5292_03830"
     CDS             170..442
                     /locus_tag="P5292_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38288.1"
                     /translation="MKKFSAMTFKNLLTIVVGGMLILMLGIGLGMHLYAKSLERETEQ
                     MARCQEPSGLCDNDEPACKDAASQMTDKALPALLRCLPGADKLMPH"
     gene            650..1210
                     /locus_tag="P5292_03835"
     CDS             650..1210
                     /locus_tag="P5292_03835"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRS38289.1"
                     /translation="MKTMFTLLAALVLQVSAAFAGTRQPVTETLQSDPYHAIVIKGDL
                     EVVLVPSENFEVAVQGTAYQVRNVSVYRNNDTLYITEVNKNGRRKSPARVEIRVKDLA
                     LLDVTGNTSVYASGHINTDILSIRANEGATVNLDVRALQVKTRVFGCSKIRLTGTTGD
                     LTCSASECKLLDISGLDVVLRQARVL"
     gene            1363..2229
                     /locus_tag="P5292_03840"
     CDS             1363..2229
                     /locus_tag="P5292_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38290.1"
                     /translation="MKTTLRLPFILIVLLTAIGPRSAHAQNPVPNPGFEEWNGGTPAG
                     WSVNNNGPVLPLTRTIDAHGGSYAVRGEVVAGTTSVAVPPLLQSQTNGVGFPLDEPIT
                     AVQCWYKCQLFSSDVFNANVYVYDAGHALVGGGSVMITQSCADFTLLNIPVTVFGSGA
                     AFAMVAMTIVDGSGTVPAHLQSSFVVDDLQALSVASGTKNLASEQAVLTVFPNPAREV
                     VRILLPPAGRNSAQSTSDLLVLDQEGREVFRQQVRQERDVAEIRLDILGWQPGTYTLV
                     LNDTPKQRFVVR"
     gene            2295..3125
                     /locus_tag="P5292_03845"
     CDS             2295..3125
                     /locus_tag="P5292_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38291.1"
                     /translation="MKKILTLLAAGLFSGGLFAQIPNAGFETWTSGNPDSWLSNNVPG
                     TYTPVTQSADVHSGTSAARGEIVDFFGSVVPPLLYVANGGGGFPVTQDYTSLIGWYKG
                     NVLNNDIIQITIAAYDASNNPVGGGSDYFGNSAGYTAFTVPIFTNGTPTASYQIFFSI
                     ADTSGAGDGTVGSYFIVDDLELTTSTDIQDRASADQLKVFPNPASSYLFYVPSGNMRG
                     QIHAQLLDLQGKSVFAENFEAAVNQQARKIDLNGIAPGFYLLRLVSGEANEVVKVVVK
                     "
     gene            complement(3186..3851)
                     /locus_tag="P5292_03850"
     CDS             complement(3186..3851)
                     /locus_tag="P5292_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HRS38292.1"
                     /translation="MTTIQPKTFAFLKALKKNNNRDWFNDHKSEYENAKDNVLGFIQE
                     LVVAFSTFDSSLRGLEAKDCLFRIYRDTRFSKDKTPYKTNLGASINAGGKKSMGPGYY
                     VHLEPGGSFIAGGIWMPPADEVKKIRQEIDYNGKDLKKVLTKPSFKKAFGGLSREHAL
                     KTAPKGYPKDHPDIELLKLNSFIVWKNVSDKDILGPKSIKTLTTLGKEMKPLMDFLKT
                     ALD"
     gene            4014..>4195
                     /locus_tag="P5292_03855"
     CDS             4014..>4195
                     /locus_tag="P5292_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38293.1"
                     /translation="MKRILFLLLLLVSNQAWSQSALLFNETRREAYLFRSNSSSSIGQ
                     VINKLAQAQGRPATSV"
BASE COUNT          908 a         1147 c         1055 g         1085 t
ORIGIN      
        1 cgcacctcgt ccctcgtccc tcgtccctcg tccctcgtcc ctcgccccac gtccctcgcc
       61 ccacgtccct cgccccacgt ccctcgcccc tcctttcaac acgcttcggt gcggaagatt
      121 aacgtttcgg aaagatttcg gatatttaaa ttgacttaat tcgccatcat tgaagaaatt
      181 ttcagccatg accttcaaga acctgctcac catcgttgtt ggtggaatgt tgatcctgat
      241 gcttgggatc ggattgggaa tgcatttgta tgcgaaatcc ttagaacgcg aaaccgagca
      301 aatggcccgt tgtcaggagc cttccggact ttgtgataat gacgagcctg cctgcaagga
      361 cgcggcgagt cagatgacgg ataaagctct ccctgcactc ttgcgttgtt tgccgggagc
      421 tgataagctg atgccgcatt gatccgccac gcaagctgtt attcttaagc aaacgttatc
      481 gtttgctttt tttatttcca cccattcttc gattcgcgaa aatttaaagg ttcgaatcgc
      541 atgtcttcgt ttcaaccagc atttctgtcg cttcggtcat ttctaccggt ctgaccgcaa
      601 ttgtcgggtg catctttgct cccgcaacac cccctcaaac cgttacgtca tgaaaacaat
      661 gttcaccctc ctcgccgcac ttgtcctcca agtcagtgct gcatttgccg gcaccaggca
      721 gccggtcact gaaaccttac agtctgatcc ttatcacgcg atcgtgatca agggagacct
      781 ggaagtcgtg cttgtaccct ccgagaattt cgaagtagcg gtacagggca ccgcgtatca
      841 ggtacggaat gtctcggttt accgaaataa tgacacgctc tacatcacgg aagtgaataa
      901 aaacggacga cgtaaatctc ctgcccgcgt ggagattcgt gtaaaagacc tggctttgct
      961 ggatgtcaca gggaatacat ccgtctatgc cagcggacac atcaataccg atatcctgag
     1021 cattcgtgcg aacgaaggcg cgacggtcaa cctggatgtt cgtgcgctgc aggttaaaac
     1081 ccgtgtattc ggatgcagca agatccgcct caccggaaca acgggcgacc ttacctgttc
     1141 cgccagcgaa tgcaagctgc tcgatatctc aggcctggat gtcgttttgc gtcaagcccg
     1201 cgtactgtaa tccctccccg gaaaccctga taatttgttt tttctcaagt gtggggctcc
     1261 gcgccgtaag gtgcggggct tttttttatt cttccggtaa tcacgcggcc tatctttctg
     1321 ttgcggtcga ggcggtactc tcccgaccgc aggccctatg aagtgaaaac tacgctgcgg
     1381 ttacctttca tactaatcgt tctcctgaca gcaatcggac cccgatccgc tcatgcacaa
     1441 aatccggtac cgaatcccgg atttgaagag tggaacggcg gcacaccggc aggctggtcg
     1501 gtgaacaaca atggccctgt gctacccctt acccggacca ttgatgccca cgggggtagt
     1561 tatgcggtac gcggagaagt ggtagccggc accaccagtg tcgctgttcc tcctctattg
     1621 caatcacaaa ccaatggtgt aggatttcca ttggacgaac cgatcacggc cgtgcaatgc
     1681 tggtacaagt gccagttgtt ttccagtgat gtgttcaatg caaacgtcta tgtgtacgac
     1741 gccggtcacg cgctggtagg cggaggatcg gtcatgatca cccagtcgtg cgcggatttc
     1801 accttgttga atattccggt taccgtgttc gggagtggag cggcctttgc gatggttgcg
     1861 atgaccatcg tggacggcag cggaactgtc ccggcccatt tgcaatcatc gtttgtcgtt
     1921 gatgacctgc aggcactttc ggtagcgagt ggaacgaaaa atctcgcgtc agaacaggct
     1981 gtgttgaccg tatttccgaa tccggcacgg gaagttgttc ggatcctgct gccaccggct
     2041 ggtcggaaca gtgcgcaaag cacttccgat ctcctcgtgc tggaccagga ggggcgggaa
     2101 gtgttccgtc aacaggtacg tcaggaacgg gatgttgccg aaatccgcct ggatatcctc
     2161 ggatggcagc ccgggacata tacccttgtc ctgaatgaca cgccgaaaca acgattcgtt
     2221 gtccggtaat taatatccga ggtcattggt ttcatgcggc aggcttggta attttacgta
     2281 ccaattctac catcatgaaa aaaatcctta cgctgcttgc agccggtctc ttctcgggcg
     2341 gtttgttcgc tcaaatcccc aatgccggct tcgaaacctg gacgagtggc aacccggata
     2401 gttggttgtc caacaatgtg ccggggacct atactccggt aacccaatct gcggatgttc
     2461 attccggcac gtccgcggcg cgcggagaaa tcgttgattt cttcggcagt gtcgttcctc
     2521 ctttgctgta cgttgccaac ggaggcggcg gattcccggt cacgcaggat tacaccagcc
     2581 tgatcggctg gtacaaaggc aatgtgttga ataacgacat cattcagatc acgatcgcgg
     2641 cctacgatgc atccaacaac ccggtcggtg gtggtagcga ttactttggc aacagtgccg
     2701 gatacaccgc tttcactgtt ccgatcttta ccaacggtac gccgacggcc tcttatcaaa
     2761 tcttcttctc cattgcggat acctccggag ccggtgatgg cacggtcggt tcttatttta
     2821 tcgtagatga tttggaactt accacatcca ctgacatcca ggatcgggct tcggctgatc
     2881 aattgaaagt gttccctaat ccggccagca gctatttgtt ctatgtgcca tccggcaata
     2941 tgcgcggcca gatccatgca caacttctcg acctgcaggg taaatcggtg tttgcggaaa
     3001 atttcgaagc ggccgtgaat caacaagctc gcaaaataga cctcaacggt attgctccag
     3061 gcttttacct gctgcgcctt gtctccggcg aggccaacga agtcgtaaaa gtcgtagtga
     3121 aataagcgtt gcacgtttga acgtttgaac gttgtaacgt tcaaacgttc aaccttcaac
     3181 tcacgtcaat ccaaagccgt cttcaggaaa tccatcaacg gcttcatttc cttgccaagc
     3241 gttgtcagtg ttttgatcga cttcggccca aggatgtctt tatcggaaac gtttttccaa
     3301 acaataaagc tgttcagttt aagaagttca atatccggat gatctttcgg gtagccttta
     3361 ggcgcggtct ttaaggcatg ctcgcgactt aacccgccaa aggctttttt gaacgaaggt
     3421 ttggtgagga ctttcttcag gtctttacca ttataatcga tttcctgccg gatctttttg
     3481 acttcatcgg caggcggcat ccaaatgccg cctgcgataa agcttccgcc gggctccagg
     3541 tgcacataat aacctggtcc catcgatttc tttccacccg cgttgatgga cgcccccagg
     3601 ttggtcttgt agggcgtctt gtctttggag aaacgcgtgt cccggtagat ccggaacaga
     3661 cagtccttcg cttccagtcc gcgcaggctc gaatcaaagg tgctgaacgc gacaaccaac
     3721 tcctggatga acccgagtac gttgtccttc gcgttttcgt attcggattt gtggtcgttg
     3781 aaccagtcgc gattgttgtt cttcttcaat gccttcagga aggcgaacgt cttgggttga
     3841 atggttgtca tgggtagtcg ggttggacga aaatctatcg gatcgcaata ccgtcagagc
     3901 cgtgatggtc aagttactat ttgcggtata atttccaact gccgttaacg ggtggtaagc
     3961 tgccacgact tattttcgct catggcacca tccttgccaa ttctgccgct cctatgaaac
     4021 gtatcctttt tctgctcttg ctcctggtgt ccaaccaggc ctggtcgcaa tccgccttgc
     4081 tgttcaacga gacccggcgg gaagcctatc tgttccggtc gaacagcagt tcgtcgatcg
     4141 gacaggtcat caataaattg gcacaggcac agggccgacc ggcaaccagt gtcga
//
LOCUS       DAOTON010000136         4194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17159_length_4194_cov_3.432955, whole genome shotgun sequence.
ACCESSION   DAOTON010000136 DAOTON010000000
VERSION     DAOTON010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17159_length_4194_cov_3.432955"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2241
                     /locus_tag="P5292_03860"
     CDS             <1..2241
                     /locus_tag="P5292_03860"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS38294.1"
                     /translation="VIAVAVNPAPVAAFSATTTTGCAPLTVSFTNSTSGSPAYSWTFG
                     DGGSATTASPAHTYTATGTYTVTLIATQGSCADTLVRTAYITIATTPTASFSTANVCL
                     GDPVSFTNSSLGNGSPITAYSWDFGDGSSPSSSASPTHNYAGAGTFAVTLTADNGSCS
                     DDTTISVTISPAPVAAISSSSLSGCAPLAVNFSNTTTGSPSYSWTFGDGGTSTATSPS
                     HTYTSGGTFPVQLIATQGSCADTASLSITVTPTPIASFTTANVCQGDSVRLNNTSGFP
                     GSPSNSISWTFGDGGTSNLGSPAHFYANAGTYTVLLDITNAAGCHDTAGALVTVNPAP
                     VVQYAASTLTGCDQLTVNFTNTTTGAVAYSWLFGDGGTSSANQPSHTYTAPGTYTVVQ
                     TATSDSGCTASRASLNMITVRATPQASFTASSQSICKDACISFTGQASAPVNGWTWRF
                     DDGQPAAASAQSPAQVCYPTTGDYAVTLVVSDGFCSDTLTQPQFITVADCSQLPVADF
                     RSSDTTICAGDCIDFVSLSLNATNWNWTFQGANTATSTIESPTGICYPNAGIYPVKLI
                     VQNLSGRDTLEVLNFITVHALPATPGFSQAGNVLTAPTATAYQWYFNGIAISGANGQT
                     YTATLSGNYAVEVFDANGCSAISPAKAVTLVGIDELQSLGMVFHVYPNPLQESLHVLI
                     HAERKLDYRIRVQDLLGQTLIFETGTLLPGDETLHFSSADWAAGVYWVIMEAENRLSA
                     RMVVRP"
     gene            2374..>4194
                     /locus_tag="P5292_03865"
     CDS             2374..>4194
                     /locus_tag="P5292_03865"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRS38295.1"
                     /translation="MPGNRPLQIAFVLLSLCFSSHRVFAENPIADSSSFYNLSLEQLM
                     NVEVTVASQVAMTAKESPGVVTVITEEELRRSGCDDLMELLQRLPGIDFGVDVEGVVG
                     IGVRGNWGHEGKVLMLIDGQEMNEELFSTLQFGRHMPLQFVRRIEIIRGPGSAIYGGN
                     AEYAVINIQTTLDEKREGITATASYGQMQRAMAGRSLGVAGGHRIGELHWNYQLQQGE
                     YNRSDRTYQDNLGDSYDMKENSDLSHGSFATSFLFRGLRVSGMLDRYHTHQRDGYEGA
                     LTQAYPTNFDSWHLNAMYEKRLGDKWTLSPGIRVKQEAPYNFTGTSAEDEFTAYDRTT
                     LKKRAFVNAQYDSGERFNLSLGASYYQLIARDRRDDGLFFNGSPSFYIDNASVFAQAI
                     IKTRPANITLGARYNYNNRFDPAFVPRIGLNRVRDGYHVKLLYSAAFRAPSVENIDAT
                     SAGIRPESTTVAEIEAGVRITRDAYLTANLYDVQTKDVIVFFYENENEDSYRNAGRTG
                     TRGAELDFRWKTNWGYLSVSHAYYTSDKGTGVEEYVVPGVSGVHLAFPAHKSVCSANV
                     RLSDRFNITPAVTGMSKRFGARYNPDNGEDEAVEFRPVVYA"
BASE COUNT          907 a         1211 c         1154 g          922 t
ORIGIN      
        1 gtaatcgcgg ttgccgtgaa tcccgcgccg gttgcggctt tcagcgccac gaccaccacc
       61 ggctgcgcac ccctcacggt gagtttcacc aatagcacga gtgggtcgcc ggcgtatagt
      121 tggactttcg gcgacggcgg cagcgcgacg accgcaagtc cggcgcacac ctataccgcc
      181 accggaacct ataccgtgac cctgatcgcc acgcagggaa gttgtgccga tacgctcgtt
      241 cgcacagcgt acattaccat cgcgacgact ccgaccgcat ccttcagcac cgccaacgtc
      301 tgtcttggtg atccggtaag ttttaccaat agcagcctgg gtaacggctc gcccatcacc
      361 gcttactcct gggattttgg cgacggttcc tccccgagct cctccgcctc gcccacgcac
      421 aactatgccg gggccgggac atttgcggtg acactcacgg ccgacaacgg aagctgtagc
      481 gacgacacga ccatcagtgt gacaatcagt ccggcaccgg tggcggcgat cagctcctct
      541 tcgctcagcg gctgtgcgcc attggcggtt aacttcagca acaccactac cggctcgccc
      601 tcctactcct ggacctttgg tgatggcggc acttctacag caaccagccc atcgcatact
      661 tacacgagtg gcggcacgtt tccggtgcaa ctgatcgcga cacagggaag ttgtgcggat
      721 accgcgagcc tgtcgattac cgttacccca acacccattg catcatttac caccgccaat
      781 gtttgtcagg gtgattcggt tcgactaaac aataccagtg gcttcccggg ctctccttcg
      841 aacagtatca gctggacctt tggtgacggc ggcacgagca accttggttc tcccgcgcat
      901 ttttacgcga atgcgggcac gtacaccgtc ttgctggata ttacgaatgc ggccggttgt
      961 catgatactg ccggcgcact cgtcaccgtc aatccggcac cggttgtaca gtatgccgcc
     1021 agtacgttga cgggatgtga tcaactgacc gtgaatttca ccaacaccac tactggcgcg
     1081 gtagcgtata gctggttgtt tggcgatggc ggaacatcat cggccaatca accgtcgcac
     1141 acctatacgg cccccggtac ctatacggtg gttcaaacgg cgacctccga cagcggctgt
     1201 acggcttccc gcgcatcgct caacatgatc acggttcgtg caacaccaca ggcatcgttt
     1261 acagcttcct ctcagagcat ttgcaaggac gcgtgtattt cctttacggg tcaggcatcc
     1321 gctccggtga atggctggac ttggagattt gacgacggac aacccgcggc cgcgagcgct
     1381 caatcgcccg cccaggtttg ttatccgacc accggggatt atgccgtaac gctggtggtc
     1441 agcgacgggt tctgcagcga cacactgaca caaccgcaat tcatcacggt agcggattgt
     1501 tcccaattgc cggtagcgga ttttcgttcg agtgacacca cgatctgcgc gggtgattgc
     1561 atcgatttcg tcagcctgtc tctgaatgca accaactgga actggacctt ccagggcgcc
     1621 aatacggcaa cctcaacgat cgaaagcccg acggggatct gttatcccaa cgcaggcatt
     1681 tacccggtga agttgattgt ccagaatctt tcaggacgcg acaccctgga ggtgctgaac
     1741 tttatcaccg tgcacgcgtt gcccgcgact ccgggttttt cgcaggcggg caatgtgttg
     1801 accgctccga cggcgaccgc ttaccaatgg tatttcaacg gcatcgcgat atccggcgcg
     1861 aatggtcaaa cctacacggc aacgctttcg ggtaattatg cggtagaagt tttcgacgcg
     1921 aatggctgtt cggccatttc acccgcaaaa gccgtgacgc ttgtaggcat cgacgaactg
     1981 caatcgctcg gcatggtttt ccacgtctac ccgaatccac tgcaggaaag ccttcatgtg
     2041 ttgatccatg cggagcgcaa gctggattac cggatccggg tgcaagatct acttgggcaa
     2101 acactgattt tcgagacagg aacccttctg cccggggacg aaaccctaca cttttcttcc
     2161 gcagactggg ccgcgggtgt atactgggtc atcatggagg ctgaaaaccg cttgtcggcc
     2221 cgcatggtgg ttcgccccta agccgacacg ctttgaatag agcacctttt cgtttttctt
     2281 acaactgtaa gatgcggtga catttcaccg gtttatatgg taccctaaga atagggtaac
     2341 gggtataatg ggaaacatca ttcccggaag cccatgccag gaaaccgccc cttacaaatt
     2401 gcctttgtcc tactgagttt atgcttcagc agccatcgcg tttttgccga gaaccccatt
     2461 gcggacagct cgtcgtttta caacctgtcg cttgaacagt tgatgaatgt ggaagtgacc
     2521 gttgcctctc aggtagcgat gactgccaag gagtcgcccg gtgtcgtgac cgtcattacc
     2581 gaagaagagc ttcgtcgatc gggttgtgat gacctgatgg aattgttaca acgattaccc
     2641 ggcattgact tcggcgtgga tgtggaggga gtagtcggca tcggcgtgcg tggcaattgg
     2701 ggccacgagg gaaaagtgtt gatgctgatc gacggacagg agatgaacga agagctcttc
     2761 tccacgcttc agttcggtag acacatgccg cttcaatttg ttcgccgtat cgagatcatc
     2821 cgcggtcctg gttccgccat ctatggtggc aatgcggagt atgcggtgat caacatccag
     2881 acgacactcg atgaaaagcg cgaaggtata accgccaccg ccagttatgg tcagatgcaa
     2941 cgtgcgatgg ccggtcgttc cctgggagta gctggaggcc atcgtatcgg tgagttgcac
     3001 tggaactacc agttgcagca aggcgagtat aaccgcagtg accggaccta ccaggacaac
     3061 ctgggagatt cgtacgatat gaaggagaat tccgacctgt cgcacggttc cttcgcgacc
     3121 agcttcctgt tccgcggttt gcgtgtcagc ggcatgctgg accgctacca cacccatcag
     3181 cgggatggtt atgaaggcgc gttgacacag gcctatccga ccaacttcga ctcctggcac
     3241 ctcaacgcga tgtacgaaaa gcggctgggc gacaaatgga cgcttagtcc gggcatacga
     3301 gtgaagcagg aggcgccgta taattttacc ggcacatcag ccgaagacga gtttaccgct
     3361 tacgatcgga cgaccttaaa gaaacgagcg tttgtcaacg cgcaatacga ttccggtgag
     3421 cggttcaacc tgagccttgg cgcgagctat tatcaactca ttgcccgaga ccggagagac
     3481 gacggtttgt ttttcaacgg gagcccgagt ttttacatcg acaacgcgag cgtgttcgca
     3541 caggcgatca tcaagacccg acccgcgaac attaccctgg gtgcgcgcta taattacaac
     3601 aaccggtttg atccggcctt cgttccgcgg atcggtctaa atcgcgtgcg cgacggctat
     3661 cacgtgaagt tgctgtacag cgcggccttc agagccccct cggtcgagaa tatcgacgcc
     3721 accagtgcgg gcatacgccc ggaaagcacc accgtggccg agatcgaggc gggtgtccgg
     3781 atcacccgcg atgcctacct tacggcaaac ctttacgatg tacagacgaa agacgtgatc
     3841 gttttctttt acgagaacga gaatgaagac tcctatcgta acgcgggccg aaccggcaca
     3901 cgtggtgcgg aattggattt ccgctggaaa acgaactggg gatacctgtc ggtttctcac
     3961 gcctattaca cctctgataa aggaaccgga gtcgaggagt atgtcgtgcc gggcgtatcc
     4021 ggtgtccacc tggcatttcc ggcccacaag agtgtttgtt cagccaatgt tcgcctgagc
     4081 gatcgcttca acatcacgcc ggcggttacg ggcatgtcca aacgttttgg tgcccggtat
     4141 aatccggata acggtgaaga cgaagccgtg gagtttcgtc cggttgtcta tgcg
//
LOCUS       DAOTON010000137         4162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17309_length_4162_cov_3.647675, whole genome shotgun sequence.
ACCESSION   DAOTON010000137 DAOTON010000000
VERSION     DAOTON010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17309_length_4162_cov_3.647675"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="P5292_03870"
     CDS             complement(<1..652)
                     /locus_tag="P5292_03870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS38296.1"
                     /translation="MPLHLYRHAFSNLNRNIWILSLAMFINRSGSMVLLFTTLYMTRD
                     LHFSIAEAGFVMSFYGMGSVLGSYLGGWLTDRKNHFDIMVSALISSGLILLSLLVITT
                     PAGMAAVIFIYALAADSFRPANAAAIAEYSTPENRTRSVGLMRLAVNLGFAVGPAVGG
                     FVALYLGYRWLFVIDACTGFLAALMLFLYLPRPATIPGRHKNEVLADSSTSAYRDLR"
     gene            871..2298
                     /locus_tag="P5292_03875"
     CDS             871..2298
                     /locus_tag="P5292_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38297.1"
                     /translation="MKKIYAIFLWFSTSLVFSEAQTPTFQIPFNNSGGTLSNELFCVE
                     SDQSGNIYFCGKHTDVITFGGTTLSVGSGGAWFGKLSPGGNLEWLRQGGTPGTTDIAY
                     GIAPDPRNNGRCYVAGSLNTSSASTFGSQTLPAGYLGFVACYDATGSALWLAGTSSAV
                     YSIACDANGYLFINTGDAAIHKLDAGNGNDLGTITLSGSLMNPQWHNIVIHGNDVIAQ
                     GGNKILKFDNNLNQLWSTPVNASLAETFRLNLDANGDVYGTFYALFGSVTVGSVTKSN
                     FPNGYVYRLDGATGNVLSCDSILINGAASKIKEYIPAAGSPQFYISGDGAFNTPTVLK
                     DGPSLSDTWTKVFPSNAPVNDLEIISNNCMVAVGKHSGTSVFDSYTLNLPAGSAGIDN
                     SYLIGLCSGTVDIPEQSDASTRAYPNPAADYIVVPGNETETIRLSNVLGEHFIVPAIG
                     SNRFDLRGLPAGVYVMPRGMRFVKQ"
     gene            2396..2884
                     /locus_tag="P5292_03880"
     CDS             2396..2884
                     /locus_tag="P5292_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38298.1"
                     /translation="MIVDIVIRETPEGEVIHYLLSEDEREFDLRGLLFEDEVTDCDWL
                     DEEQRFLVAAYNDWEGKSNFAILSLHGELLRKGIRQIRQYIPEQRLFIIEQTGASLGE
                     DRFDYQAEEDDILFAVMNDTGDYVIEPQPEQIFYNEQETAFGVGSGQEWIIYDLKGKR
                     IS"
     gene            2884..3351
                     /locus_tag="P5292_03885"
     CDS             2884..3351
                     /locus_tag="P5292_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38299.1"
                     /translation="MDTALLSGLKAGLHQRCLELLASKIVELTRMQTELLEGALDDSK
                     SSAGDKHETARAMMQLEQEKLAGQLQDLLDQQQQLIRFDPSLSDGVIRPGSLVQTDRM
                     LFYLTVGLGKLRLETTECMVLSPSSPLGKRMNGLSAGDRCEVNGQVYHILAVV"
     gene            3446..>4162
                     /locus_tag="P5292_03890"
     CDS             3446..>4162
                     /locus_tag="P5292_03890"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRS38300.1"
                     /translation="MRTILFSMLWMLPGLTRLLAQSDPIAVSGLYSLGNDFQDRAYAV
                     TVDDSGNTVMSGSASSAMVNFDLLGGSTPGEHPVPGNYLVRYRADQSVQWARYSGYSE
                     SRFVITHLETDRMNHTLVLGYYRGLVDIDFGQSGTEYLQEANYQPSFFLAQLDSNGKP
                     IRHVEWVALSDSFDTYTGDRTFVTDLSLDENGNIYLAGYSGSVFTTQQLPNDTLREFT
                     WFVIKLDSSLQVQWMDGLEKV"
BASE COUNT          961 a         1123 c         1155 g          923 t
ORIGIN      
        1 aacgcagatc gcggtaggca gaagtgctgc tgtcggccag cacttcattc ttgtgacgtc
       61 cgggtatcgt tgcaggccgg ggaaggtaga ggaacaacat gagcgcggcc agaaaaccgg
      121 tgcaggcatc gatgacgaac agccagcgat agccgaggta gagcgcgacg aatccgccaa
      181 cagccggacc aacggcaaag ccgaggttga ccgccaggcg catcagcccg accgaccggg
      241 tgcggttttc aggcgtgctg tattcggcga tggcagcggc gttggccggt cggaaactgt
      301 cggccgccag cgcatagatg aagatcaccg ccgccatacc cgccggcgtg gtgatgacca
      361 gtaaggagag caggatcagt ccgctcgaga tcagcgccga caccatgatg tcgaagtgat
      421 tcttgcgatc ggtcagccag ccgcccagat aactgcccag tacgctgccc atcccgtaga
      481 agctcatgac aaatcctgcc tccgcgatcg agaagtgcag gtcgcgcgtc atgtagagcg
      541 tggtaaacaa caacaccatc gacccgctgc ggttgatgaa catcgccagc gagaggatcc
      601 agatgttgcg gttcagattc gaaaaagcgt gacggtaaag atggaggggc atgggagaga
      661 atggtaaaga taggcaatgt gggattgatg ggggatcgtt gacagaggtg atttgaaatt
      721 ggtaactggt aattagcaat tagtaattag taattaaaaa tcggtgttta gttactaatt
      781 actaattacc agttaccagt taccaaaata cccaccagca cggatacccg tgctcctttt
      841 cccggttacc tttgcgtaaa cacctcaccc atgaaaaaaa tctacgccat cttcctttgg
      901 ttctcgacct ccctcgtatt tagcgaggcc caaaccccga cgttccagat cccgttcaac
      961 aattccggag gcactttgtc caatgagtta ttctgtgtgg aatctgacca atccggcaac
     1021 atctatttct gtggtaagca taccgatgtg atcaccttcg gtggcaccac cttgagcgta
     1081 ggcagtggcg gagcctggtt cggaaaactg agccctggcg gcaacctcga atggttgcgg
     1141 cagggtggca cacccggcac caccgacatt gcttatggca ttgccccgga cccgcgcaac
     1201 aacgggcgtt gctacgtggc cggttcgctg aacaccagct ccgcttccac cttcggaagt
     1261 cagaccctgc cggccggtta tctgggattc gttgcctgct acgatgctac aggtagcgcc
     1321 ttgtggctgg caggaaccag ttcggcggtc tattccatcg cctgcgacgc gaacgggtac
     1381 ctgttcatca ataccggtga cgcggccatc cacaagctgg acgcggggaa cggaaacgat
     1441 ctgggcacca tcaccctgag cggtagcctc atgaaccccc aatggcacaa cattgtgatc
     1501 cacggcaacg atgtgatcgc gcagggaggg aataagatcc tcaaattcga caacaacctg
     1561 aatcaactct ggagtacgcc ggtgaatgca tccctggccg agacgttccg gctcaacctg
     1621 gatgcgaacg gcgatgtgta tggcaccttc tacgcactct ttggtagtgt gaccgttggt
     1681 agcgtgacga aaagcaattt tccgaacggc tatgtttatc gcctcgacgg cgcaaccgga
     1741 aacgtgctgt cctgcgattc gatcctgatc aacggggccg cctcgaagat aaaagagtat
     1801 atcccggctg ccggcagtcc gcagttttat atcagtggcg acggcgcttt caacacccct
     1861 acggtactga aggatgggcc ttcgctttct gacacctgga ccaaagtgtt cccttccaac
     1921 gcgccggtga atgatctgga aatcatttcg aataattgca tggtggcggt cggcaaacat
     1981 tcgggcacat cagtgttcga cagttacacg cttaacctcc ccgcgggatc tgccggcatc
     2041 gataacagct acctgatcgg gctttgttcc ggaaccgtcg acatccctga acaatccgac
     2101 gcaagtacca gggcctatcc aaatccggct gccgattaca tcgttgtgcc gggaaatgaa
     2161 acggagacga ttaggctttc gaatgtcctg ggcgaacact tcatcgtgcc ggcgatcggc
     2221 agcaatcgct tcgatctgcg cgggctgccg gcaggagttt atgtcatgcc ccgtggaatg
     2281 cgcttcgtaa aacaataagc gcagtaccgg aaagaccgca tcccttccgt tcgtcgtcct
     2341 gcaggagccc gttccgcgtg gccggtctcc gaaaagcgcc tacctttgcg ctcgcatgat
     2401 cgtcgatatt gtcattcggg aaacaccgga aggtgaagtc atccattacc tgctgagcga
     2461 agacgaacgc gagttcgatc tgcgcggttt gctgttcgag gacgaagtta ccgactgcga
     2521 ctggctggat gaggaacaac gcttcctggt ggccgcttat aacgactggg aaggcaaatc
     2581 gaatttcgcg atactgagcc tgcatggtga attgttgcgg aaagggatcc ggcagatccg
     2641 gcagtacatt ccggaacaac gcctcttcat catcgagcaa acgggagcca gtcttggcga
     2701 ggatcgtttc gattaccagg cggaggaaga cgacatactt ttcgcggtga tgaacgatac
     2761 gggcgactac gtgatcgaac cgcagccgga acagatcttt tacaacgaac aggaaaccgc
     2821 cttcggcgtc ggtagcggac aggagtggat catttacgat ctcaagggga agcgcatttc
     2881 ctgatggaca cggctctctt gtcgggttta aaagccggtc tgcaccaacg atgtctggag
     2941 ctgctcgctt caaagatcgt cgaacttacc cggatgcaaa ccgaactgct ggaaggcgcg
     3001 ctggatgaca gtaaaagttc cgcgggcgac aagcacgaaa ccgcgcgcgc gatgatgcag
     3061 ttggagcagg aaaagcttgc cggacaattg caagacctgc tcgatcagca acaacagctt
     3121 atccgatttg atccgtcgtt atcggacggg gtgatccgtc ccggaagtct ggtacaaacg
     3181 gatcgcatgc ttttttatct caccgtcggg ctcgggaaac tgcgactgga aaccactgaa
     3241 tgcatggtcc tctccccttc ctccccgctc ggcaaacgca tgaacgggct ttccgccggc
     3301 gatcgatgtg aagtcaacgg gcaagtgtat cacattctgg cggtcgttta gatttttcgc
     3361 tattttcagt catcgccgaa ttgtcattta acaagatcct gtctcttgat gtaccaatga
     3421 attccgatct tcggctaact cttccatgcg cacgatcctg ttttcgatgc tctggatgtt
     3481 gccgggtctg acgcgactgc ttgcacagtc ggatccgatc gcggtgtccg ggctttattc
     3541 actgggcaat gatttccagg accgcgccta tgcggtcacc gtggatgata gcggcaacac
     3601 tgttatgtcc ggttccgcct ccagtgctat ggttaatttc gatctgctgg gaggaagtac
     3661 gccgggcgag cacccggttc ccggcaatta cctcgtccgc tatcgggcgg accaatcggt
     3721 gcaatgggct cgttactccg gctattcgga atcacgtttc gttatcacac acctggagac
     3781 tgatcgaatg aatcacacgc tcgtcctggg ctattaccgg ggtctggtgg atattgattt
     3841 cggtcaaagc ggaaccgaat acttgcagga ggccaactat caaccatcct ttttcctggc
     3901 gcaacttgac agcaacggaa aaccgatccg gcatgttgaa tgggtggccc tgtcggatag
     3961 tttcgacact tataccggtg atcggacctt tgtaaccgat ctttcactcg acgaaaacgg
     4021 taacatctat ctggcggggt actcgggaag tgtttttacc acgcagcagt tgcccaacga
     4081 tacgcttcgt gaattcacct ggtttgtgat taagctggac agttcattgc aggtccaatg
     4141 gatggatggt ctggaaaagg tt
//
LOCUS       DAOTON010000138         4149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17373_length_4149_cov_3.053249, whole genome shotgun sequence.
ACCESSION   DAOTON010000138 DAOTON010000000
VERSION     DAOTON010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17373_length_4149_cov_3.053249"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="P5292_03895"
     CDS             <1..916
                     /locus_tag="P5292_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRS38301.1"
                     /translation="PRVIDINAVGRWLLVNGIIAPFRAPKSAKLYKEIWTEQGSPLLY
                     YTRRQAELLQAALGTNYRVDFAMRYQSPSIQAVLEKFRQSGVSDITILPLFPQYASAT
                     TGSVHEKVMETVGRWQAIPEIRFISSYCDLPEFIDGFVALGRKYDPSKYDHVLFSFHG
                     LPERQIRKADDHGHCLKDNCCAELGPRNANCYRAQCFQTARKIAAGLGLSFEQYTVCF
                     QSRLGKDPWVRPYSDDIIRERAKKGDKRLLVFAPAFTSDCLETIHEIGVEYDELFREL
                     GGEKIQLVESLNESELWVRAMRKLVSRH"
     gene            1048..2694
                     /locus_tag="P5292_03900"
     CDS             1048..2694
                     /locus_tag="P5292_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38302.1"
                     /translation="MRIARLRLLLSSLLLISLGTRAQPMLREWTTYHDGNLNGYDVAR
                     DIQITDSQMVVVTGTSFHLFSGGNFTTLLLDELGNVRWKDHYYSAETGVLNGGQRIRT
                     ASGNVFYAVGTLAFRAGDISLMKYGPTGRRWTQNFTPSWFSDYLDEGIDLALDGDENL
                     YAIGVATSTAGNLNDVYTIKCDSSGQVIWEEDYSGASGDDFPARILTGPSGGCHSLSG
                     SFNFFGSGNYDFTLLEYNTTGTLVNTRYLDGPGNRSDHPRDITSTLGGWYVCGTTATT
                     SNNNDALIGLLNDNGTAGWRQTYAGTANQGDTAIQILETQTGDVVALLHSKELLNGSS
                     RDAIVVRCYDAGGNLRWSWTTFGNDSLGALPAEMLLDATGTIMLCGSYTSSGQQSNGL
                     LAAIDANGQELWMTGFDGGFNQHDGFTGIAIDTIGGIYVCGYSRNGIANSRYVIAKYS
                     FNGTTGIPMSDAKRNIRVFPQPAPPGSIISLELGETLPPDVYSLLMTVEGKTVCVMEK
                     FEVNDRVLRLQLPATIAAGSYLLVTSTAAQTFRQTILVQP"
     gene            2691..>4149
                     /locus_tag="P5292_03905"
     CDS             2691..>4149
                     /locus_tag="P5292_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38303.1"
                     /translation="MIRSSLLCLSILAALNTVHADHHVAMDLTYRATGVQDQYELTVK
                     FFRDCNGMAMPADVVVCFNSVSCNFQGSVTLNRVSGPTLVPFPSCVVPPPGASSCSGG
                     PVYGVQQSVFRGLLTLPYACSDWKLEYVQCCRNYAITNVQNAGTESFYLLATLDNLNF
                     PGNSSPAYATIPVTMWCTGTPFIYDHGVTETDGDSLSFEFTSPMAKSNLVPCDVNVLP
                     YPVTFTGGLSATQPFYTVAGTTIDPANGVVRCIANQVQIGVMDILVKEWRNGQLAGTT
                     RRDIQVYFGATCQASIPEFVTGVVNGVPNAVAVTCGDTSVLLNIDRDIYCHSAALDGT
                     DFLALDVNQVPLTVTRAEPVNCGSVFTRTVRLYFAQPLQAGVSVVWTRNGTDGNTLIG
                     SCSDAMLLNDSVNLVAAPCNPLNAGFAAGLAGLCLNGCTDFTDQSLGNPTSWSWTFQG
                     ADPPTSTLQNVPSVCYDSAGVFDVRLIISNGSNTDT"
BASE COUNT          904 a         1218 c         1133 g          894 t
ORIGIN      
        1 cccgcgggtg attgacatca atgcggtggg gcgttggttg ttggtgaatg gcatcatcgc
       61 accattcagg gctccgaaat cggctaaact gtacaaggag atctggaccg aacagggctc
      121 tcccctcctc tattacacac gccgacaggc ggagttatta caagccgcgc tgggaaccaa
      181 ctatcgcgtg gatttcgcga tgcggtatca gtcaccgtcg atccaggccg tgctcgaaaa
      241 attccggcag tcgggtgtat ccgacatcac catacttcca ttatttccac agtacgcttc
      301 ggcaacgacc ggttcggtgc acgagaaggt gatggaaacc gtcggtcgct ggcaggcgat
      361 cccggagatc cgatttatca gcagctactg cgacctaccg gagttcatcg acggatttgt
      421 cgcgctgggc cgcaagtacg atccttcgaa atacgaccac gtattgttca gcttccatgg
      481 ccttcccgaa cggcagatcc gaaaggcgga cgatcacggg cattgtctga aagacaattg
      541 ctgtgcggaa ctgggaccgc gcaacgcgaa ctgttaccgt gcccagtgtt tccagactgc
      601 acgaaagatc gcggcaggac tgggattatc gttcgagcag tataccgtct gttttcaatc
      661 gcggctgggc aaagatccct gggtgcgtcc gtactccgac gacatcatcc gcgagcgcgc
      721 caaaaaaggc gacaaacgct tactggtgtt tgctccggca ttcacgagcg attgcctgga
      781 aaccattcac gagatcggcg tggagtacga cgaattgttc cgcgaactcg gaggcgagaa
      841 gatccagttg gtggagagtt tgaatgagtc ggagctttgg gtacgcgcga tgcgtaagct
      901 tgtgagccgg cattaacatt tccgacattt tagcgtgact tattccgacg ggcccgcatc
      961 ccgttcggac agcacttttt ccggttcagc gtttttcatt ctgccaaata agcggtgcag
     1021 gtacatcttg gcgcaaaatt caacatcatg cgcattgcaa gactccgcct actcctgtca
     1081 tcactgctgc tgatcagcct gggcacaaga gcgcagccca tgcttcggga atggaccacc
     1141 tatcatgacg ggaatctcaa cgggtacgat gtagccaggg acattcagat taccgatagt
     1201 cagatggtcg tcgtaacggg tacgagtttc catctcttct caggcggcaa cttcaccacc
     1261 ctgctgcttg atgaactcgg gaatgtccgt tggaaagacc actactacag tgcggaaacc
     1321 ggcgtgctca acggcggaca acgcatccgt accgccagtg gaaacgtctt ctacgcggtc
     1381 ggaacgctgg cgttccgagc aggcgacatc agcctgatga aatatggccc gaccggtcgg
     1441 cgctggacgc agaatttcac acccagttgg ttcagcgact atttggacga aggcatcgac
     1501 ctggcgctgg acggtgatga aaacctctac gcgatcggtg tggccacctc gactgccggc
     1561 aacctcaacg acgtctatac gatcaagtgc gacagcagtg gccaggtgat ctgggaagaa
     1621 gactacagcg gagcgtcggg cgacgacttc cctgcacgga tattgaccgg ccccagcggt
     1681 ggttgtcatt ccctttcagg atcgttcaac ttcttcggaa gcggtaacta cgacttcacc
     1741 ttactggaat acaatacgac gggaaccctg gtcaataccc ggtatctcga cggccccggc
     1801 aaccgaagcg atcacccgcg cgatatcaca tcgaccctgg gcggatggta tgtctgcggg
     1861 accacggcaa ccaccagcaa caacaacgac gccttgatcg gcctcttgaa cgacaacggt
     1921 acggccggct ggcgacagac ctatgccgga accgcgaatc agggcgacac ggccatccag
     1981 atactcgaaa cccaaacggg cgatgtggtc gctttgctgc atagcaaaga actcctgaac
     2041 ggcagttccc gcgatgccat cgtggtacgc tgctacgatg cgggtggcaa ccttcgttgg
     2101 tcctggacca cctttgggaa tgattcgctg ggcgcacttc cggcagaaat gttactcgac
     2161 gcgacgggca cgatcatgct ttgcggaagc tacaccagca gcggtcagca aagcaacgga
     2221 ctgttggccg ccatcgatgc gaacggccag gagctttgga tgaccggctt cgacggcggc
     2281 ttcaaccaac acgacggatt caccggcatt gcaatcgata caatcggcgg catttacgta
     2341 tgtggatata gtcgcaacgg aatcgccaac agccggtatg tgattgcgaa atactccttc
     2401 aatggaacta cgggtatacc catgtcggat gcgaagcgaa acatccgtgt atttccacaa
     2461 cccgcgcccc ccggatcgat catctccctg gaacttgggg aaaccctccc tcctgatgtt
     2521 tactcgttgc tgatgacggt cgagggcaag acagtctgcg ttatggaaaa gttcgaggtg
     2581 aatgatcgcg tcctccgcct ccaactccct gcaacgatcg ctgccggctc ttacctgctc
     2641 gttacgtcta ccgctgcgca aacatttcgt caaaccatcc tcgttcagcc atgatccgat
     2701 cctcactgct ctgcttgtcc atactggccg ccctgaatac agtacacgcc gatcaccatg
     2761 tcgctatgga tcttacctac cgggcgaccg gagttcagga tcagtatgaa cttacggtca
     2821 agttcttccg ggattgcaac ggcatggcga tgccggcgga tgtggtcgtc tgcttcaact
     2881 cggtcagttg caatttccag ggatccgtca cgctcaatcg tgtttcgggt ccgacgctgg
     2941 tgccattccc ttcgtgcgtg gtgccgccac ccggcgccag cagttgcagc ggcgggcccg
     3001 tgtatggcgt gcaacaatcg gtctttcgcg ggttgctcac gctaccgtat gcatgcagcg
     3061 actggaagct ggagtatgtg cagtgctgcc ggaattatgc catcaccaat gttcagaatg
     3121 ccggaacgga aagtttctat ttactggcca cgctcgacaa cctgaacttc cccggcaaca
     3181 gctcgcctgc ttacgcaacc atcccggtca ccatgtggtg taccggcact ccgttcatct
     3241 acgaccacgg agttacagaa acggacggag attcgttaag tttcgaattc accagtccga
     3301 tggcgaagag caacctggtt ccctgcgacg tcaacgtact cccctatccg gtgacattca
     3361 ccggtggctt atccgccaca cagcccttct acacggttgc aggcaccacg atcgatcctg
     3421 ccaacggagt agtgcgttgc atcgccaatc aggtacagat cggcgtcatg gatatattgg
     3481 tgaaagaatg gcgaaacggt cagcttgccg gaacaacccg gcgggacatc caggtgtact
     3541 ttggcgctac ctgtcaggct tcgattccgg agtttgtgac cggagtcgtc aacggcgtac
     3601 ccaacgcggt agcggtcacc tgcggggaca ccagcgtgtt actgaacatc gatcgtgaca
     3661 tctattgcca ttccgctgcc cttgacggca cggatttcct ggcgctcgat gtcaaccagg
     3721 ttcccttgac cgtcacccgg gcggagccgg tcaactgcgg cagcgttttc acccgcaccg
     3781 ttcgcttata cttcgctcaa cccttgcagg cgggagtatc ggttgtatgg acccgcaatg
     3841 gcaccgacgg caacaccctc atcggctcct gctccgacgc gatgctgctg aacgattccg
     3901 tcaacctggt ggcagcgcct tgtaatccgc tcaacgcggg gtttgcagca ggacttgccg
     3961 gactttgcct gaacggctgt accgacttca ccgatcaatc gctgggcaat ccgacgtcct
     4021 ggtcctggac tttccagggc gcggacccgc ccacgagcac cttacaaaac gtgcccagtg
     4081 tatgctatga ttccgccggc gttttcgatg tgcggctgat catttccaac ggtagtaata
     4141 ccgatacac
//
LOCUS       DAOTON010000139         4128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17459_length_4128_cov_2.893199, whole genome shotgun sequence.
ACCESSION   DAOTON010000139 DAOTON010000000
VERSION     DAOTON010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17459_length_4128_cov_2.893199"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..1481)
                     /locus_tag="P5292_03910"
     CDS             complement(192..1481)
                     /locus_tag="P5292_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38304.1"
                     /translation="MLTYALVPGYVALFVWLIGRSGFFAATGVPVRWFQGVFLLKVLA
                     GCVLGWLYTYHYTDRTTSDTFKFFDDSGILFSALTERPWDFFRMFTGIGGNDPELRHY
                     YESMNAWLNKDVLFNDNKTIIRLNTFFRFFSMGNYYVHVVFINFLSLTGLTGLYRSFT
                     STLPGRGRILFVTTFLLPSVVFWGSGLLKDGLLLFALGTLVYVYRQLMLGDRRWSRLA
                     LFGISFLLLLFTKFYVIVTITPGLIAWWMSRGKSLSGALLRFGAVYGVFFVLAFNLHR
                     IVPAYKLADIIYYKQQNFYVLAEMTKAKSVIRAPEFYATGWSLAAHAPLGFLTTLLRP
                     TVFDAQGNLLVMASAIENALLLVLMLVLLARPRRVEPTALSFVLFAGIYVLLLYSLIG
                     LITPILGAMVRYKVVGMPFLFFVLVAFSSVWGRRASK"
     gene            1870..4104
                     /gene="purL"
                     /locus_tag="P5292_03915"
     CDS             1870..4104
                     /gene="purL"
                     /locus_tag="P5292_03915"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRS38305.1"
                     /translation="MSETLEQAVTVETAKELGLLPEEFDKIKEYLGRTPNFTELSIYS
                     VMWSEHCSYKNSIVWLKTLPKDGPHMLVQAGAENAGLVAIGDGLACAFKIESHNHPSA
                     IEPYQGAATGVGGINRDIFTMGARPVAQLNSLRFGNLSLDKTRWLLRGVVKGIGDYGN
                     AFGVPTVGGELFFDDCYNINPLVNAMSAGIVEVGKTISATSYGVGNPVYIVGSATGKD
                     GIHGATFASGDLHEDSAEDLPSVQVGDPFTEKLLLEASLEVIASGAVVGMQDMGAAGI
                     ICSTSEMSAKGKHGMVIHLDKVPTRQKHMQAWEILLSESQERMLIVVKRGREPEVRKI
                     FDKWDLNCVQIGEVTSGTQLKFFMDGEMVADVPADSLVLGGGAPVYHREYSVPSYFYE
                     NKRFRIDEIEEPEDLREVGQFLLRHPNIASRRWVYRQYDSMVGTVNMSTNAPSDAAIV
                     NLKRSDKALALTVDCNSRYVHADPEQGCAIAVAEAARNIVCSGGEPSAITNCLNFGNP
                     YNKEVYWQFVNAIKGMGKACRKFQTPVTGGNVSFYNQSSDEGPVFPTPTIGMLGVLQS
                     KSHRMTLDFKRAGDSIFLIGESVNDINSSEYLYSWHKRKNTPAPHFDLDTEYAVQQVV
                     KQLIRKGLVASAHDVSDGGLFIALAESGFPNNLGFSIETDFDCRADAFLFGEAQSRVV
                     VSVNEQHIDAFVDYLSTVNNVTWSNLGTVTEGEVLIDEQSWGSIGEMKAEYETVIEKM
                     MNVE"
BASE COUNT          966 a         1304 c         1128 g          730 t
ORIGIN      
        1 caactgatgt ccgtcaccgg caggccccgc ttccgcttcc cgtaaaatat tcccgacaga
       61 tttttttcga aatagatcca taaaagtcgt gtggacttgt tctttcaaat ataggtttta
      121 ttgcagtcgt tgcggatgct tgcagaaagc gatccgcgtt catccgccaa gtccgcggaa
      181 tccgcgtgca atcacttcga agcccgccgc ccccaaaccg aactaaaagc caccagcacg
      241 aagaacagga acggcatgcc cacgaccttg taccgcacca tggctcccag gatcggcgtg
      301 atcaatccga tgaggctgta gagcagcagc acgtagatcc cggcgaatag cacgaagctc
      361 aaagccgtcg gttccacccg ccgcggcctt gccagcaaga ccagcatcag cacgagcagc
      421 agcgcattct cgatcgccga agccatcacc agcagattcc cctgcgcgtc gaacactgtc
      481 ggccgcaaca aagtcgtcag gaaacccagc ggcgcgtgcg cggccaggct ccagcccgtc
      541 gcatagaact ccggcgcccg gatcacgctc ttagccttgg tcatctccgc cagcacgtag
      601 aagttctgtt gtttgtagta gatgatgtcg gccagtttat acgccggaac gatccggtgc
      661 aggttgaatg ccaacacaaa gaatactcca tacaccgccc cgaagcgcag caaagctccg
      721 ctcaacgact tcccccggct catccaccag gcgatcagtc cgggcgtgat ggtgacgatc
      781 acgtagaact tcgtgaacaa caacaacagg aaactgatac cgaagagcgc cagtcgcgac
      841 cagcgtcggt cgcccagcat cagttggcga tacacataca ccagcgtccc cagggcaaag
      901 agcaagagcc cgtccttcag caaacccgat ccccagaaca cgaccgacgg caggaggaaa
      961 gtcgtcacga acaggatacg gccgcgaccg ggcagggtgg acgtgaagct gcggtagagt
     1021 cctgtgagtc cggtcagcga gaggaaattg atgaacacca cgtgcacgta gtaattcccc
     1081 atcgagaaaa accggaagaa cgtattcagc cggatgatgg tcttgttgtc gttgaacagc
     1141 acgtccttgt tcagccaggc gttcatcgac tcgtaataat gccgtagctc cggatcgttt
     1201 ccgccgatgc cggtgaacat gcggaaaaaa tcccagggcc gctcggtcag cgccgagaac
     1261 aggatgccgc tatcgtcgaa gaacttgaac gtatcggagg tcgtccggtc cgtgtaatga
     1321 taagtgtaca gccacccgag gacgcaaccc gccagcacct tcaacagaaa caccccctgg
     1381 aaccagcgca ccggcacacc cgtcgcagcg aaaaacccgc tcctcccgat cagccagaca
     1441 aacagggcca cgtaccccgg gacaagcgcg taggttaaca tcgtgggtaa agttaggaaa
     1501 tgacgtgggc aaaagtgttc cgggttccga gttccgagtt acttgaaaaa gggaggaggc
     1561 ttgcctgccg gagctttagc gaaggcaggg acgagggagg agaaagagga gggtggcgga
     1621 atgagtcgga ttatttcacc ggagcaataa aggggtatca ggatcaagct tgcaaaattc
     1681 ccccgatgag tcaataggac tttcgtattc aaaggattag tcgccccaag cagcaggcag
     1741 gccgctttta actttcaacc ttcaaccttc aactttcaac tttcaacttt caaccttcaa
     1801 ctttcaactt tcaaccaacc cggaacctaa aacctagttg ctacctttgc ctccaaacca
     1861 cccccaccca tgtccgaaac actcgagcag gcagtgactg tggaaacggc caaagaactg
     1921 ggcctgcttc cggaggaatt cgataagatc aaggaatacc tgggccgtac gcccaacttc
     1981 accgaactca gtatctactc ggtgatgtgg agcgaacact gctcctacaa gaactccatc
     2041 gtctggctca agacgctgcc gaaagacggt ccgcacatgc tcgtgcaggc cggtgcggag
     2101 aatgccggtc tggttgccat cggcgacgga ctcgcctgcg ccttcaagat cgaatcgcac
     2161 aaccatcctt ccgccattga accctaccag ggcgcggcga cgggtgtggg cggcatcaac
     2221 cgcgacatct tcaccatggg cgcccgcccg gtggcgcagc tcaacagcct gcgcttcggt
     2281 aacctgtcgc tcgacaagac ccgctggctc ttgcgtggcg tcgtgaaagg catcggcgac
     2341 tacggcaacg ccttcggcgt accgacggtc ggcggtgaac tcttcttcga cgactgctac
     2401 aacatcaacc cgctcgtgaa tgccatgtcg gcgggcatcg tggaagtcgg caagacgatc
     2461 tccgctactt cctacggcgt cggtaacccg gtgtacatcg tcggttccgc cacgggcaag
     2521 gacggcatcc atggcgccac cttcgcctcg ggcgacctgc acgaagattc cgccgaagac
     2581 ctgccgtctg tacaggtcgg tgatccgttc accgaaaaac tgctgctcga agccagcctc
     2641 gaggtgatcg cctccggcgc ggtggtcggc atgcaggaca tgggcgcggc gggcatcatc
     2701 tgctcgacca gcgagatgtc ggccaagggc aagcacggca tggtcatcca cctcgacaag
     2761 gtgccgacgc gccagaagca catgcaagcc tgggagatcc tcctgagcga atcccaggag
     2821 cggatgctca tcgtcgtcaa gcgcggccgg gaacccgagg tgcgcaagat ctttgacaag
     2881 tgggacctca actgcgtgca gatcggagaa gtgacttccg gcacgcagct caagtttttc
     2941 atggacggcg aaatggtcgc cgacgtcccc gccgactccc tcgtgctggg cggcggcgct
     3001 ccggtctatc accgcgagta ttcggtgccg agttatttct acgagaacaa gcgcttccgc
     3061 atcgacgaga tcgaagagcc ggaagacctg cgcgaagtcg gacagttcct gctccgccac
     3121 cccaacatcg cctcgcgccg ctgggtgtac cggcagtacg actcgatggt cggcacggtg
     3181 aacatgagca ccaacgcgcc ttccgacgcc gcgatcgtca acctcaagcg ttccgacaaa
     3241 gccctcgccc ttacagtcga ttgcaatagc cgctacgtgc acgccgaccc cgaacagggc
     3301 tgcgccatcg ccgtggccga agccgcgcgc aacatcgtct gctccggcgg tgaaccttcc
     3361 gccatcacca actgcctcaa cttcggcaat ccgtacaaca aggaagtgta ctggcagttc
     3421 gtcaacgcca tcaagggcat gggcaaggcc tgccggaagt tccagacacc cgtcaccggc
     3481 ggcaacgtga gcttctacaa ccagagttcc gacgaaggcc cggtcttccc gacgccgacg
     3541 atcggcatgc tcggcgtgtt gcagagcaag tcgcaccgca tgacgctcga cttcaagcgc
     3601 gcgggcgaca gcattttcct catcggcgaa tcggtgaacg acatcaacag ctccgagtac
     3661 ctgtatagct ggcacaagcg caagaacacc cccgcgccgc acttcgacct cgacaccgag
     3721 tatgccgtgc aacaggtcgt gaagcagctc atccgcaaag gcctcgtagc ctcggcgcac
     3781 gacgtatccg acggcggcct cttcatcgcc ctggctgaat cgggtttccc caacaacctc
     3841 ggcttcagca tcgagaccga tttcgactgc cgcgccgacg ccttcctctt cggcgaagcc
     3901 cagagccgcg tggtcgtcag cgtcaacgaa caacacatcg acgccttcgt cgactacctc
     3961 tccaccgtca acaacgtcac ctggtccaac ctcggcaccg tcaccgaagg cgaagtcctc
     4021 atcgacgaac aatcctgggg cagcatcggc gagatgaaag cggagtatga gacggttatt
     4081 gagaagatga tgaatgtcga gtgaggttta gggttccggg ttcccagc
//
LOCUS       DAOTON010000140         4124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17478_length_4124_cov_3.028754, whole genome shotgun sequence.
ACCESSION   DAOTON010000140 DAOTON010000000
VERSION     DAOTON010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17478_length_4124_cov_3.028754"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..440)
                     /locus_tag="P5292_03920"
     CDS             complement(96..440)
                     /locus_tag="P5292_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRS38306.1"
                     /translation="MKDQVQEFNAYRSRMNERILSQDNLVLKRLFNLDTNTYAEGALS
                     VKTKELLGLVASMVLRCDDCIRYHLGRCHEEGVTTEELFETFAVANIVGGTIIIPHLR
                     RAVEYWDQLQSA"
     gene            complement(447..1280)
                     /gene="tatC"
                     /locus_tag="P5292_03925"
     CDS             complement(447..1280)
                     /gene="tatC"
                     /locus_tag="P5292_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010177112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRS38307.1"
                     /translation="MALFDRSNINPQAEMSFLGHLEALRWHLVRAVGVVMVLALTWFF
                     LKELLFDGILLAPKNPQFLTYRILCHLSDRLQLGEDLCIREIPFRLIATDISSQFTTH
                     MWVAFVAGLVTGFPYVVWELWRFIKPALHEKERRYANGIVFYTSFLFITGVLFGYYII
                     TPMTVNFLGNYQVSTEVQNMISLDSFISTVTTMTLITGIVFELPIVVYFLSKVGILSP
                     KFMRDYRKHAIVVILILSAVITPTSDATTLIMVAIPLYILYEVSIFVSAYVVRGKQAV
                     Q"
     gene            complement(1331..2296)
                     /locus_tag="P5292_03930"
     CDS             complement(1331..2296)
                     /locus_tag="P5292_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRS38308.1"
                     /translation="MKNQSNIRKLAVRTLQIEAGAVANLTQHIDDQFVACVEAILKSK
                     GRVIVSGIGKSAIIAQKIVATLNSTGTPSIFMHAADAIHGDLGIIQKHDVIICISKSG
                     DTPEIKVLVPLLRSWGNILIGMVGNPESYLAKQADLVLNTYVAKEACPNNLAPTSSST
                     AQMAMGDALAVCLLEQRGFTSRDFAKYHPGGALGKKLYLKVKDIFPGNAKPAVSSNTD
                     IKDVILEISSNRLGATAVLEKGKLVGVITDGDLRRMLINRKSIEGIKARDVMNTNPKQ
                     VAKDTMAVDALNLMKQYNISQLLVTDKGRFAGFVHLHDLVKEGIL"
     gene            2429..>4124
                     /gene="recQ"
                     /locus_tag="P5292_03935"
     CDS             2429..>4124
                     /gene="recQ"
                     /locus_tag="P5292_03935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRS38309.1"
                     /translation="MLVAEKPLKTYLRKYFGFDKFKGEQESIIRNVLSGKDTFVIMPT
                     GGGKSMCYQLPSMIMEGTAIVVSPLIALMKNQVDAMRGFSNEEGIAHFLNSSLNKSEI
                     ATVRKDIKEGRTKLLYVAPESLTKEENVEFLRSVPISFFAIDEAHCISEWGHDFRPEY
                     RRLRPIIDAIGKVPIIALTATATPKVQLDIQKNLGMTDATVYKASFNRGNLYYEVRPK
                     VDVIKEIIKFVKQNTGKSGIIYCLSRKKVEEVASTLSVNGIRALPYHAGLDAGVRADT
                     QDKFLMEEIEVIVATIAFGMGIDKPDVRFVIHHDIPKSLEGYYQETGRSGRDGREGKC
                     VTFYSYKDIEKLEKFMKGKPVAEQEVGKQLLLETVGYAETSVCRRKVLLNYFGEQYVE
                     ENCGNCDNCLHPKKSFDGKEEVSTVLETVQAVKEKFPMEHIVNVVCGKAENNVKRHGH
                     DQLEIFGEGSDHEPRFWTAIIRQSLLAGLLSKDIETYGTLRLTDKGKAFLKKPVKIMV
                     VEDKDYSNTESDEEEEFSGSNAAAADPVLFAQLKDLRKQIAKQKSVPPFVVFQDPSL"
BASE COUNT         1059 a          986 c         1156 g          923 t
ORIGIN      
        1 tcccaggaag aaaagcagga aaaggaataa acgtacgacc atgcgaaagc ccggaacagg
       61 gggaatcatg cccgaaaaat acgtgttgaa aaggatcagg ctgattgcag ttggtcccag
      121 tattcaacag cccggcgtag atgcggaatg ataatggtgc cgccgacaat attggcgaca
      181 gcaaaggttt caaacagttc ctccgttgtt actccctctt catgacagcg gccaaggtga
      241 tagcgaatgc agtcgtcaca gcggagcacc atgctggcaa cgagaccgag caattccttg
      301 gtctttacag ataaggcacc ttcggcgtag gtgttcgtgt caaggttgaa cagccgtttc
      361 agaacaagat tatcctgcga aaggatgcgc tcgttcatcc ggcttcggta cgcgttgaat
      421 tcctggacct ggtctttcat ggtgttttac tgaacagctt gtttgccccg aaccacgtag
      481 gcgctgacga agatggaaac ttcgtaaagg atgtagaggg ggatggcgac catgatcagc
      541 gtcgtggcgt ccgaggttgg cgtgatcacg gccgacagga tcaggatcac cacgatggca
      601 tgtttccggt agtcgcgcat gaatttagga ctcaggatac ccaccttgga aaggaaatag
      661 accacgatcg ggagttcgaa cacaatgccg gtaatcaggg tcatcgtggt tacggtggaa
      721 atgaacgagt cgaggctgat catattctgg acttccgtgc tgacctggta attgcctaga
      781 aaattgacgg tcatcggcgt aatgatgtag tagccgaaga gtacacccgt gatgaagagg
      841 aacgacgtgt aaaacacgat cccgttggcg taccgtcttt ccttctcatg cagcgcgggt
      901 ttgatgaagc gccagagttc ccataccaca tagggaaagc ccgtgaccag gcccgccacg
      961 aaagcgaccc acatatgggt cgtgaactgc gaagaaatat cggttgcgat cagccggaag
     1021 gggatctcgc ggatgcacag atcttccccc aactgcaagc ggtctgacag atggcacagg
     1081 atacggtagg taaggaactg agggttcttg ggggcaagca gtattccgtc aaagaggagc
     1141 tccttcagga aaaaccaggt cagcgccagc accatgacga caccgaccgc gcgtaccagg
     1201 tgccagcgta aggcctccag atgcccgaga aaagacatct ccgcctgcgg attgatgtta
     1261 ctgcgatcga aaagagccat gaagggtcaa agatagccag tgaattcaaa tcgcccggag
     1321 gactgtgtta tcacaggatg ccttccttga cgagatcatg caggtgcacg aagcccgcga
     1381 atcgaccctt atcggtcacc aacagttgtg aaatattgta ctgcttcatc aggttgagcg
     1441 cgtccactgc catggtgtcc ttggcaactt gcttgggatt cgtattcatc acatcgcggg
     1501 ccttgatgcc ttcaatggat tttcggttga tgagcatccg gcgaaggtcg ccgtccgtta
     1561 tcaccccaac caatttgccc ttttcaagca cagccgtcgc gcctaaacgg ttcgatgaga
     1621 tctcgagaat cacatccttg atgtccgtat tggaggaaac ggcgggtttc gcattgccgg
     1681 ggaatatgtc cttgactttc aggtaaagct ttttccccaa ggcgcctccg ggatggtatt
     1741 tggcaaagtc gcggctggtg aagccccgtt gctcgagcag acagactgcc agcgcgtccc
     1801 ccatagccat ctgcgcggtg gaactgctgg ttggagccag gttgttagga caggcctcct
     1861 tggccacata ggtattcagg acgaggtcgg cctgtttggc caggtacgat tcgggattac
     1921 cgaccatccc gatcaggatg tttccccagg aacggagcaa gggcaccagc actttgatct
     1981 caggagtatc accacttttg gagatgcaga tgatcacatc gtgcttttga atgatgccaa
     2041 ggtcgccgtg gatagcgtcg gcggcatgca tgaaaatgga aggtgttccg gtcgaattaa
     2101 gcgtcgcgac gatcttttga gcgataatcg cactcttgcc gataccgctg acaatcacgc
     2161 gtcctttgct ttttagaatt gcctcaacgc aggccacaaa ctgatcgtca atgtgttgcg
     2221 tgagattggc cacagctccg gcttcgattt ggagcgtcct tacggccagt ttgcggatgt
     2281 tggattgatt tttcaatgat ctagcttaaa aatatcgcgc gggaatagcc gtttattggg
     2341 acaaaattat ttaattttaa agcaggaacg cgtgatgcct tgctcacgcg taattgtttt
     2401 gtaaaaagct gaaaaacaga atactataat gctagtggcg gaaaaaccgt tgaagactta
     2461 cctcagaaag tacttcggtt ttgacaaatt taagggggaa caggagagca tcattcgaaa
     2521 tgtgctgtcg gggaaggaca ccttcgtcat catgccgaca ggtggtggga aatccatgtg
     2581 ctatcagctt ccttccatga tcatggaggg aactgcgatt gtcgtttcac ccctcatcgc
     2641 cctgatgaag aaccaggtcg atgccatgcg gggattcagt aacgaagagg ggatcgcgca
     2701 ttttctgaac tcctcactta ataaatcaga gatcgcgacg gtccgcaagg acatcaagga
     2761 gggtcgaacc aagctcctgt acgttgcccc ggagtcactg acaaaagagg aaaacgtaga
     2821 gtttctgcgc agtgtaccca tctcgttttt cgcgatcgat gaggcgcact gtatttcgga
     2881 atgggggcat gacttccgac ccgaataccg ccggcttcgc ccgattattg acgcgatcgg
     2941 taaagttccc atcattgctc ttacagccac ggcaacgccc aaggtccaat tggacattca
     3001 aaagaacctg ggtatgaccg acgcgacggt ctataaagcg tccttcaacc gcggaaacct
     3061 gtactatgag gttcgcccga aggttgacgt catcaaagag atcatcaaat tcgtcaagca
     3121 gaacaccgga aaatccggaa tcatctattg tcttagcagg aagaaagtgg aagaggtagc
     3181 atccacgctt tccgtgaatg gcattcgtgc attaccgtat catgccggcc tcgacgccgg
     3241 cgttcgtgcc gatacccagg ataaattcct catggaagaa atagaggtca tcgtggctac
     3301 gatcgccttt ggaatgggta tcgataaacc ggacgtccgt tttgtcatcc accacgatat
     3361 acccaagagc ctggaagggt attaccagga gaccggacgt tccggcaggg atggtcgcga
     3421 agggaaatgc gttaccttct acagttacaa ggatatcgag aagctggaga agttcatgaa
     3481 gggaaaaccg gtggccgagc aggaagtggg taaacagctg ttgcttgaaa ccgtcggcta
     3541 tgcggaaacg tccgtttgca gaagaaaagt gttgctcaat tatttcggcg aacaatatgt
     3601 cgaggagaat tgcggcaatt gtgataactg cctccacccg aagaagagtt tcgacggtaa
     3661 ggaggaagta agcactgtgc ttgagaccgt tcaggcggtg aaggagaaat tcccgatgga
     3721 gcacatcgtg aatgttgttt gtggaaaagc ggagaacaat gtgaagcgcc atgggcacga
     3781 ccagctcgag atattcggcg aggggtcgga ccatgagccg aggttctgga cagccatcat
     3841 ccggcaatcg ttgctggccg ggctgttgtc gaaggatatt gagacctacg gaacgcttcg
     3901 gttgacggat aagggcaagg cctttctcaa gaagcctgtc aagatcatgg tggtcgaaga
     3961 caaggactac agcaacacgg aaagtgatga ggaagaggag ttcagcggaa gtaatgctgc
     4021 cgctgccgac ccggtcctgt tcgcgcaatt gaaagacctg cgcaagcaga ttgccaagca
     4081 gaaaagcgtt ccgccctttg tggtctttca ggatccttcg ctgg
//
LOCUS       DAOTON010000141         4122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17486_length_4122_cov_3.542169, whole genome shotgun sequence.
ACCESSION   DAOTON010000141 DAOTON010000000
VERSION     DAOTON010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17486_length_4122_cov_3.542169"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="P5292_03940"
     CDS             <1..894
                     /locus_tag="P5292_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRS38310.1"
                     /translation="LLPILAFIYQGSAVLRSLQRTNLAMVPVYLMLPVLLGIASLLHY
                     YKYGRLTADAVFALYILCGILVSIVLNRWLNRYVHRRLDQAEPEFERKTWMPVAASFL
                     VATLLNLALKQADVLYVGHLLNHTKAGYYGAAARMASLVALGLSAADFVYMPRIAALF
                     QQEKKRALQEMLRQASRQILLISVPVILLLAIAGKMLLGWFGDSFRDAYLPLVILLAG
                     QAVNAATGMVGGVLSMTGHHRIFVLFNLVAFLVQLTLSWLLIPSFGMTGAAIATSTAL
                     IALNLLGYTYLRRKLDLVASV"
     gene            931..2196
                     /locus_tag="P5292_03945"
     CDS             931..2196
                     /locus_tag="P5292_03945"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRS38311.1"
                     /translation="MLVESAAAPDLSIRSTWFDRLLIFLLVFSIYFEANLPFVGRAST
                     PFLLFAFTLAYLSFYRGRSLLRQLSSRYFIVSLLFALTCLFMENLHPDPTYDFITRFL
                     NMTIGIFCIAVLCRDLVAFDIALFTFVLASALQSVILITGTFNILHQFSADGFYDASR
                     VRLQAFEEFYLRGNLNDISYFSSIGAIIGIIWSYFERETWKKVLLLSLTFPSILGVFL
                     PASRTGAVIFFASMLVFLWRSGFSLRRWVIPALTLLVFLIVVVPEVVWVRLGSLMRFS
                     ELQEQDSRTRVYGAVLRNFDDYVLTGVGSGHYWDGWAVASGITDRFSPQVAAAAHNAF
                     FQVWIYWGLPALILFFLLIREYARAVDTRIEGDRRKSAIFVFALMIPMIFLFYHSFYH
                     KSFSIGLGMLLGTRMWELFDKERREEVKA"
     gene            2193..>4122
                     /locus_tag="P5292_03950"
     CDS             2193..>4122
                     /locus_tag="P5292_03950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS38312.1"
                     /translation="MNQLHWTFVLGSLRTGGAERATLNLANALIRRGHRVSLVLLHED
                     GTLREQLNPAVAVHVLPVDRARRAVPSLTILLERLQPDLLVAVQNHMQWAVLRANNRL
                     KQSVPLILNEQSSLDENLRRQGWKGWLLKRWLKGSWKRIAAWTAVSEASAESLRKAFP
                     AAGLLIRAIPNPAVTDELASLSNEAVDHPYFRSGTRVIVAVGRLVESKNFPLLLKAIA
                     RLRDEVKLRCIILGDGPERNRLKGLVTQLGLDDIVDLPGAVSNPYAWIRKSHLFVLCS
                     DYEGMPLALIEAMALGCPVVSTDCPGGSGEILRQGDRIYGELVPVGDDAALAQAIKKT
                     LRSPIPSNVLQDAVKDFHANRVAIRYEDLAAELLPERSQHLVMLVGPVAPPRGGVSTH
                     LERLAAHAGAGPFRFIIADFRKRVLIGQERRATGFVALLHYFRKAEIVHLHLAHPVKL
                     LIACWARMSGKKLVYTHHNSRELQRFDTRWTMRLAHQVVLVTPGALEDVRIPEERVQV
                     IPAYLPSRPRSGVGAGVAARLQGYRHVMLSSAFHLPGRPSMLYGRDIYGFDLLLQAAL
                     NFPDAVREGTCIVLLDPAGTMRATYEPLLQQLRTAGIDCWYPEEEIHLPSILPWVTVY
                     VRPTRSDGDALAVREALAA"
BASE COUNT          747 a         1153 c         1163 g         1059 t
ORIGIN      
        1 cttttaccca tccttgcgtt catttatcag ggtagtgccg tactgcgatc cttgcagcgt
       61 acgaacctgg ccatggttcc ggtgtacctg atgttgccgg tcttgctcgg gatcgcttca
      121 cttctgcatt actataagta tgggaggctg acggccgacg cggtctttgc cctgtatatc
      181 ctctgcggca tattggtcag tatagtgctc aaccgctggc tcaaccgata cgtgcatcgt
      241 cgccttgatc aggctgagcc ggagttcgag cgcaagacct ggatgccggt agccgccagt
      301 ttcctggtgg cgacgctgct caacctggcc ctgaaacaag cggacgtact gtatgtagga
      361 catttgctca accataccaa ggccggatac tatggagcag ccgcccgcat ggcatcgctc
      421 gtagcgctgg gattgtcggc cgctgatttt gtttacatgc cccgtatcgc ggccttgttc
      481 caacaggaaa agaaaagagc cttgcaggaa atgctgcgac aggcgagccg tcagatattg
      541 ttgatctccg tgccggtgat tctgttactg gcaatcgcgg gaaagatgct gttgggttgg
      601 tttggcgatt cgttccggga tgcctatttg cccttggtga tcctgctcgc cggtcaggcg
      661 gtcaatgcag ctaccggcat ggtaggcggt gtgttgagca tgaccgggca tcaccgcatt
      721 tttgttttat tcaatctggt tgcttttctc gttcagttga cgctgagttg gttgctcatt
      781 ccgtccttcg gcatgaccgg cgcggcgatc gctacttcaa ccgcgctgat tgccctgaat
      841 cttttgggtt acacgtacct gcggcgtaag ctcgacctgg tggcttccgt ctgaacaacg
      901 cccgtcgctt ttgactaact tagcggcaag atgcttgtag agtctgcggc cgcaccggat
      961 ttatccattc gatccacctg gttcgaccgc ttgttgattt tcctgctggt tttcagcatc
     1021 tacttcgaag ccaatctgcc ctttgtcgga agggcgagca ctccgtttct cctcttcgcg
     1081 ttcacgctgg cgtacctgag tttttacagg gggcgcagcc tgctcagaca attgtccagc
     1141 cgatacttta tcgtttccct cttattcgcg ctgacgtgtc tgttcatgga gaacctgcac
     1201 ccggacccga cctatgattt catcacgcga ttcctaaaca tgaccatcgg cattttctgt
     1261 atcgcggtac tttgccggga cctggtcgca ttcgacatcg cgctcttcac cttcgtcctg
     1321 gccagcgctt tgcaatcggt gatcctgatc accggtacat tcaacatcct ccaccagttt
     1381 tcggcggatg gtttttacga tgcttcccgt gttcgactac aggcctttga ggagttttac
     1441 ctgcgcggca acctcaatga tatctcttat ttctcttcca tcggagccat catcgggatc
     1501 atctggtctt acttcgaacg ggaaacctgg aaaaaggtac tcttgctgtc gctgacgttc
     1561 ccgtccattc tgggtgtctt tcttccggcc agccgtaccg gcgctgtcat cttcttcgct
     1621 tccatgctcg tgttcctgtg gcgttcgggt ttcagccttc gccgttgggt gattccggca
     1681 ctcacccttc tggtattcct gatcgtggtc gtgccggagg ttgtctgggt acggctcggt
     1741 tcactcatgc gtttttctga attgcaggaa caggattccc gtacaagggt ttacggagcg
     1801 gtgctgcgta acttcgacga ctatgttctg acgggtgttg gatccgggca ttactgggat
     1861 ggatgggcgg tcgcttcggg gattaccgat cggttctcac cgcaagtggc ggcggccgca
     1921 cacaacgcat tctttcaggt gtggatctat tggggtttac cggcgctgat cctcttcttt
     1981 ctgctgatac gggagtacgc cagggccgta gacacgcgga tcgaaggcga ccggaggaag
     2041 tccgccatct ttgttttcgc attgatgata ccgatgatct tccttttcta ccacagcttc
     2101 tatcataaat cgttttccat cggtcttggt atgctgttgg gcacacgcat gtgggaactc
     2161 tttgataagg aacggaggga ggaagtcaaa gcatgaatca gttgcattgg accttcgtgc
     2221 tcggatccct gcgtaccggt ggtgcggagc gcgctaccct taacctggcc aatgcactga
     2281 tacgtcgcgg acatcgcgtc agccttgtgc tcttgcatga ggatggcacc ctgcgtgagc
     2341 aattgaatcc cgctgtagct gtgcacgtgc ttcccgtcga ccgtgcacgc agggcagtgc
     2401 cctcactgac catcctgctg gaacgacttc agcccgacct gctggtggcc gttcagaatc
     2461 atatgcaatg ggccgtcctc cgtgcgaaca accggttgaa gcagtcggtt ccgctcatcc
     2521 tgaatgaaca gagctcgctc gatgaaaacc tgcgacgaca aggctggaag ggttggttgc
     2581 tgaagcggtg gttgaaaggc agttggaaaa gaatcgcagc ctggacagct gtgtccgaag
     2641 catcggctga aagtctccga aaggcatttc cggcggccgg tctgctaata cgcgccattc
     2701 cgaatcccgc cgtcaccgat gaactcgctt ccctcagcaa cgaagctgtc gatcaccctt
     2761 acttcaggtc cggaacgcgc gtcatcgtcg cggttggccg cctggtggag agtaagaact
     2821 ttcccttact gctcaaggcg attgcgcgtc ttcgtgacga ggtcaagttg cgttgtatca
     2881 tcctgggcga tggacctgaa cggaacaggt tgaaaggtct cgtcacgcaa ctgggtctgg
     2941 acgacatcgt cgatttgccg ggcgcggtta gtaaccccta cgcctggatc cggaaaagtc
     3001 acttgttcgt cctctgttcc gactatgaag gaatgccgtt agcgcttatc gaggccatgg
     3061 ccttgggttg cccggtcgtc agcaccgatt gtccgggagg ttccggcgaa atccttcgac
     3121 aaggcgaccg gatttacggt gagctcgttc ccgttggtga tgatgccgcg ctggcacagg
     3181 caatcaagaa gacgctgcgt tcaccgatcc cttcgaatgt gttgcaagat gcggtaaagg
     3241 actttcacgc caatcgtgtc gcgatccgtt atgaagatct cgcggccgaa ctgttgccgg
     3301 agcgcagtca acacctggtg atgctggtcg ggcctgtcgc tcctccccgc ggaggcgtgt
     3361 ccacccatct cgaacgcctg gcagcacatg ccggcgcggg tcctttccgt ttcatcattg
     3421 ctgacttccg caaaagggtg ctcatcggac aagaacgccg tgctacaggc ttcgtcgcgt
     3481 tacttcatta tttccggaaa gcagagatcg tgcacttaca tcttgcgcat ccggtcaaat
     3541 tgctgatcgc atgttgggca cgcatgagcg gaaagaagct cgtgtacacg caccacaaca
     3601 gccgggagtt gcagcgcttt gatacgcggt ggacgatgcg gcttgcccat caggtggtgc
     3661 tcgtcacccc gggcgctctg gaagacgtac gcattccgga agaacgggtg caggtgattc
     3721 ctgcttatct gccttcccgt ccgcgttccg gtgttggcgc cggtgtggcc gcgcgcctgc
     3781 aaggataccg gcatgtaatg ctctccagcg cctttcatct tccggggcga ccatcgatgt
     3841 tgtatggccg ggatatttac ggttttgacc tgttgctgca agccgcgctg aattttccgg
     3901 atgccgtccg ggaaggaaca tgtatcgtgc tcctggaccc tgccggtacc atgcgggcaa
     3961 cctatgagcc actgttgcag caactgcgga cagcaggaat cgattgctgg taccccgaag
     4021 aagagattca cctgccttcc atcttaccct gggtcacggt gtatgtgcgt ccgacccgat
     4081 cggatggtga tgccctcgcg gttcgggaag cgctggcagc cg
//
LOCUS       DAOTON010000142         4087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17669_length_4087_cov_3.536210, whole genome shotgun sequence.
ACCESSION   DAOTON010000142 DAOTON010000000
VERSION     DAOTON010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17669_length_4087_cov_3.536210"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="P5292_03955"
     CDS             complement(<1..977)
                     /locus_tag="P5292_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38313.1"
                     /translation="MNISKLTFVILIVLSHKTVGQPIQWNVPNRQQCGLANGSFEVIN
                     GHLLKRDQAGASIWMKQVGSGTEHIAASSSASVYGVTGQRVYKLDGNGNLMWCLNFSV
                     PPNPFNHEPTRLGGLAISGNRLFVQEYQCPPPPIMTFATIDDGVIIIDTNGVFQGVYN
                     KSRVTGKMEYRGCRSSPDGGVWLFNESGNSGTGVEMLHVDSLGNSYPSQYYGIQHGHY
                     TTLADFHIMPDSTLVTATEGYFDIMVMDPPEQSINITKQQPDGTPVWSYYYSDSVRAP
                     YGYGNYPRSLTSDSSGNIYLVSVTYRDNGTGIFDIGPSVIGLDSNGSVFL"
     gene            1687..3150
                     /locus_tag="P5292_03960"
     CDS             1687..3150
                     /locus_tag="P5292_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRS38314.1"
                     /translation="MAQKVHTLSLNLTMALMRSISQVDRFDAEWTSIEQRERQSLREL
                     RAIATVRSVGASTRIEGSSMTDAEVEVLLDALKVSRLEERDQQEVAGYFGALSVIIES
                     FNDIDITEANIKNLHKILMRHSEKDVWHSGNYKQISNAVEAKHADGSKWLLFKTTEPG
                     IETERAMRKLIEWYRDDQETLPIVKSAIFVYDFLSIHPFQEGNGRLSRLLSTLLLLKQ
                     GYKWIQYISFEHEIESRKAEYYEVLMQTQRKRPGENVDQWVGFFLSCLVNIQELLKNK
                     LKASTYSYSLGPKERSIVSFIANRPGSRSGQIAKSLQIPLPTIKRILNGLVENKVIAR
                     HGIGAGTNYVVEDQAVERTGRMFKLTDRNRNAEFTLRTGNSYLEIYKIILTPLFTWDR
                     PEEWSKRLLNQGLCFVLKVYTSSGGTYQDSYPIGSFVSPMHYEPIFNLTDALNIPLSV
                     TMRPLRLNEYPIRVEVELTGSMEKLDFDVLFVYNERS"
     gene            3250..3723
                     /locus_tag="P5292_03965"
     CDS             3250..3723
                     /locus_tag="P5292_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRS38315.1"
                     /translation="MSDSIHHYYLTTTWKGNRGTGTSDVGAYDRLHTVEVPGKPVLTL
                     TTDNAQYGDRSVLNPEDLLVTALSSCHMMSYLYLCAKARVVVTAYVDRAEGKMIEWEK
                     GGGRFEEVVLKPEVTVQDASMLERARQLHEMAHKICYIANSVNFPVRCDAVVRVA"
BASE COUNT         1101 a          889 c         1009 g         1088 t
ORIGIN      
        1 agaaagacac tgccattgga atctaatccg attaccgatg gtcctatgtc gaatatgcca
       61 gtgccattgt ccctataagt aacggaaacg aggtagatgt ttccggaaga atcggatgtg
      121 agggatcgcg ggtaatttcc gtatccgtat ggagccctga cactgtcact atagtagtat
      181 gaccaaacgg gcgtcccgtc gggttgttgt ttggtgatat taatggattg ctccggcgga
      241 tccataacca tgatatcaaa gtatccttca gtagcggtga ccaatgtgga atcaggcata
      301 atgtgaaaat cggccaatgt ggtgtaatgt ccatgttgaa ttccgtaata ttgagatgga
      361 taggagttgc ctaaggagtc gacgtgcagc atttcaacac ctgttccgct gtttcccgat
      421 tcattaaata accacacgcc accgtctgga gacgatctgc aacctcggta ttccattttg
      481 cccgtgaccc ggctcttatt atagactccc tgaaaaacac cgttggtgtc gataataatc
      541 actccgtcat caattgttgc aaatgtcata ataggcggtg gaggacattg atattcctgg
      601 acaaataagc gatttccgct gatggccaga ccacctaacc gtgttggttc atggttaaag
      661 ggattgggcg gtaccgagaa gttgaggcac cacatgaggt taccgtttcc atccagtttg
      721 taaacccttt ggccagtgac cccataaaca gaagctgaag atgatgcggc aatatgttca
      781 gtcccggagc caacctgctt catccagatc gatgcgcccg cctgatctct tttcagtaaa
      841 tgcccgttga taacctcaaa cgaaccatta gcaaggccgc attgttgtcg gtttggaaca
      901 ttccattgaa ttggttgtcc tactgtcttg tgagaaagta caatcaagat cacgaaggtc
      961 aatttcgaaa tattcattgt gattattttt tgtaaacctg tgaattatat cattgattca
     1021 cgaattattg tcaactgaaa atatccaatt cacctatcga aaccggcgct tcatcaattt
     1081 tcgcagcata tccaaaagaa atcgattcca gtcagccgaa aagcatgtaa gcagcaactg
     1141 cgatcctctc tacctttgca cctctttccg accacttaca ccactaccgt ctaaacacca
     1201 cctgggaagg aatcttggta gctccgctga aatccatgca ttgtttatcc cgatagtcac
     1261 ttagcgatca atagggggag gctaacaagg ttgtgattca aattataata aaacggattg
     1321 ccggtgaagg tccatacagg actcaatatt caagtaagtc gaatcccgga tacacgtcaa
     1381 caatagggat tgccggagcg attttgtaat tcggttctgg aaatcttcca gcaagatcgg
     1441 ggaatctaaa gtctgatggc gctaacgaat caacccgtga cgacataagt tggtcattag
     1501 ccggttttat ttggcgtcta ctgtcgttta ggaaagattc aggcttcgat ttattgcctg
     1561 attcactttt tatgcgtttt atcggctttt agcctaagag attgtatctt ttcgtatcaa
     1621 atcgtatcat tttttagttg atttttcgaa tctggtatcg tatatttacg tatcatatcg
     1681 tatcaaatgg cgcaaaaagt ccatacacta tccctcaatt taaccatggc gctgatgagg
     1741 agcatcagcc aggtggatcg ttttgatgct gaatggacca gcatcgagca gcgtgagcga
     1801 caaagcctgc gagaattgag agcaatagcg acagtcagaa gtgtcggagc atcaactcga
     1861 attgaaggct ccagcatgac ggacgcggaa gtggaggttc tgctggatgc tttgaaggtg
     1921 tcgagactcg aagaacgaga tcagcaggaa gtggccggat actttggagc gttgagcgta
     1981 atcatagaat ctttcaatga cattgatata acagaagcga atattaagaa tctgcataag
     2041 atcctgatgc gtcatagtga aaaggatgtt tggcacagtg gaaattacaa gcagatttca
     2101 aatgcggtgg aggcaaagca cgcggacgga agcaaatggc ttctttttaa aacgactgaa
     2161 cccggaattg aaacggaacg ggcgatgaga aagttgattg aatggtatcg ggacgatcag
     2221 gaaacgttgc ccatcgtgaa gtccgcaatt tttgtctacg attttctgag tattcatcca
     2281 tttcaagaag gcaatggccg actgagcaga ttgttgagca ccctcttatt gctgaagcag
     2341 ggatataaat ggatccaata catcagtttc gaacatgaaa tcgaaagcag gaaagcggag
     2401 tattatgaag tgctgatgca aacccagcgg aagagaccag gggaaaatgt tgatcaatgg
     2461 gtcggtttct ttctgagctg tttagtgaat attcaggagt tgctgaagaa taagttgaaa
     2521 gcatcgacat attcgtactc cttggggcct aaagaaagaa gcattgtttc attcattgcg
     2581 aatcgcccgg gaagtagatc cggccaaatt gccaaaagct tacaaatacc attgccaaca
     2641 ataaagagaa tattgaatgg cttggtagag aataaagtga ttgcaaggca tggcattggc
     2701 gctggtacaa attatgtcgt ggaagatcag gctgttgaaa ggacagggag aatgttcaag
     2761 ctcaccgata ggaaccggaa tgcggagttc accttgcgga ccggcaactc ctaccttgag
     2821 atctataaga tcatcttgac cccgcttttt acttgggata gaccggaaga gtggtcaaag
     2881 agattgttga atcaaggcct gtgttttgtt cttaaagttt acaccagtag cggaggaacg
     2941 taccaggatt catatcccat tggttcattc gttagtccaa tgcactatga acccatattc
     3001 aatttgacgg atgctttaaa catcccattg agcgttacca tgaggccgct tagattgaat
     3061 gagtatccga ttagggttga agttgaactc acgggatcaa tggagaagct tgactttgat
     3121 gtactgttcg tgtacaacga acggagttag gagacgcctg atgaatggtg gtagcttcca
     3181 atggtgctcg ggaatggatc agccgccggt tcaagtaaac gctatcgcaa tcctgaatac
     3241 atttgtcgca tgtctgactc cattcaccac tattatctca ccaccacctg gaaaggcaac
     3301 cgcggcaccg gcacttcgga tgtcggcgct tacgatcgct tgcatacggt tgaggtcccg
     3361 ggaaaaccgg tgcttacgct cacgaccgac aacgcccaat acggcgaccg cagtgtgttg
     3421 aacccggaag acctgctggt gacggccttg tcgtcctgtc acatgatgtc gtacctctac
     3481 ctctgcgcga aagcgcgggt ggtggtcacc gcttatgtgg atcgggcgga ggggaagatg
     3541 atcgagtggg agaagggggg aggccgtttc gaagaagtcg tcctgaaacc ggaggtaacc
     3601 gtgcaagatg cttccatgct cgaacgcgcc aggcagttgc acgagatggc gcacaagatc
     3661 tgctacatcg ccaactcggt gaatttcccg gtgcggtgtg atgccgtggt tcgcgtagct
     3721 tgaaccctcc gatgaatttc ttgtgttttc tttgaaaacg gcatctgtag cgggtttccg
     3781 ggcccataat ttgacgcctg cttccttttt aatatattgc gtatgtatac gctttctaac
     3841 ttagtacgtt caattttcac caccggagcc gattgttgct gatgaatctt tccgcagcac
     3901 gacgtacgaa aaacgactga gccattcggc gttaccaacg gtcggactgt tccttccgct
     3961 tgcttaaagg tcttgttaac tacttccact atgcggaacc tctatattat tgttctgttc
     4021 gtactgttca atccttcaac ttcactggcc caacctgaat tgtggtcgat gagcccgcgg
     4081 ggcgggg
//
LOCUS       DAOTON010000143         4070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17743_length_4070_cov_3.930511, whole genome shotgun sequence.
ACCESSION   DAOTON010000143 DAOTON010000000
VERSION     DAOTON010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17743_length_4070_cov_3.930511"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="P5292_03970"
     CDS             <1..1567
                     /locus_tag="P5292_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS38316.1"
                     /translation="CFPRSLFIPFAIALLYAVHPLHTEVIANVKSRDEILSMLFFLLT
                     LRSALLYARGGKWQHLPILALSFFAALLSKESAITYLAAVPLALYFFSEAKGSHYRGV
                     MVSMAVPAVLFLLIRASILGQYQSAPYAMVDNHLIGIKDFVTQRTSAIYLLGVYLYKH
                     FIPHPLICDGSYAAFDPAGPGDWKFILSFLIHIGALVYAIMNFKKRDPIAFGILFYFV
                     TISIISNVLMLIGTNYAERLLFVPSLGWCIVVAVLLDRLAGWLSPQSGTTPVPLVLTA
                     LVVLGFSSLTLARNQDWHDETRLFNTDLKKVPRSAHMRFYVGNHISAEDYLTTLPDSA
                     AIKAALVESIAQLDTAISIWEPYGDAWQRRAYNKYVLNNYPAAEQDFIRALEINPTNP
                     VAHNNYGNLLFNTRRYDLALQHFEQAVKYNPRYSHAWNNLASVYGVYGEGAREQAMNN
                     PAERDRYLADARQKWETAIGFFKKAIEQEADYAVPYRMLGFTYRNLGDEAMANKYFRE
                     ADEIAKKKANAKN"
     gene            1554..2561
                     /locus_tag="P5292_03975"
     CDS             1554..2561
                     /locus_tag="P5292_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS38317.1"
                     /translation="MLKIDIHTHILPSELPDFRKKFGYGGFVSLEHHKPCCARMMIDG
                     KFFREIQDNCWDPEVRMRECAHHQVGVQVLSTIPVMFSYWAQPQDALDLSMFLNDHIA
                     DIVQRYPKRFIGLGTIPLQAPELAIRELERCMKIGLAGVQIGSHINDWNLNEPALFPV
                     FEAAQELGAAIFVHPWDMMGKEKMNKYWLPWLVGMPAETSLAICSMIFGGIFERLPRL
                     RVAFAHGGGSFPATFGRIEHGFRVRPDLVAVDNPESPRKYLKQFWLDSLVHDPVMLDY
                     LVRFMGPERIALGTDYPFPLGELEPGKLIGEMPYSNDIKERLLHGSALEWLGLEKRLF
                     A"
     gene            2595..3341
                     /locus_tag="P5292_03980"
     CDS             2595..3341
                     /locus_tag="P5292_03980"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HRS38318.1"
                     /translation="MTRIVRMLTVFFLLNLAATAQTSSFRSGFAVLELFTSESDANSP
                     AGDAVFQEVIREARKAKQPVIGLAFHVDYWNKGSWKDPFSRNAFTFRQENYSRAFPQK
                     ELYTPQLIVNGRAQMTASSRDSVKAAIKKALISSGDPGFRLQVDSVLRDTLFLTAITP
                     KPGVNWVVRVAVTEDGLKSEVKAGPNKGKILEHDAVVRLWYSTGAKSKSLAVKVPLKG
                     LKLNDRMRLVGLLQEKKTMKILGASESGFD"
     gene            complement(3658..>4070)
                     /locus_tag="P5292_03985"
     CDS             complement(3658..>4070)
                     /locus_tag="P5292_03985"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRS38319.1"
                     /translation="EKETSRWSFNYQILIARKFSPSFSFQIAPWYNHYNMVTSISEQN
                     DSYGASALFRLKFSKRSAITAEYAYRANTYSNTTYYDSFGVGYEIETGGHVFQVHLTN
                     SFGLVENQFLPYTDTQWNDAGIRLGFNVSRVFTL"
BASE COUNT          895 a         1074 c         1122 g          979 t
ORIGIN      
        1 ctgtttcccg cgatcgcttt ttatcccgtt cgcgatcgcg ttgttgtatg cggtccaccc
       61 cctgcacacg gaggtgatcg ccaatgtgaa gagccgcgac gagatcctca gcatgttgtt
      121 cttcctgttg acactccgct cggctttgtt gtatgcccgg ggtggcaaat ggcagcacct
      181 gccgatactg gcgctttcct tcttcgcggc actgttgtcg aaggaatcgg cgatcaccta
      241 tctcgccgcc gtgccgctgg cgttgtactt tttctcggaa gcgaagggta gccactatcg
      301 cggggtcatg gtctccatgg cggtaccggc ggtattgttc ctcctgatcc gggccagtat
      361 tctcgggcag tatcaatctg caccgtatgc aatggtggat aaccacctca tcgggatcaa
      421 ggactttgtc acccagcgta cctcggcgat ctatctcctg ggcgtttatc tgtacaagca
      481 ttttatccca cacccgctta tctgtgacgg atcctacgcg gccttcgatc cggcgggtcc
      541 cggcgattgg aaattcatcc tgtcattcct gattcacatc ggcgcgttgg tttacgcgat
      601 catgaatttc aaaaagcgcg accccatcgc cttcggtatc ctgttctact ttgttacgat
      661 ctccatcatt tccaacgtgc tgatgctcat cggtaccaac tatgccgaac gcctgttgtt
      721 tgtcccttca ctgggttggt gcatcgtcgt agcggtgtta ctggatcggc ttgccggctg
      781 gttgagtccg cagtcgggga caaccccggt acccctggtg ctgacagccc tggtcgtact
      841 cggattttcc tcgctgaccc tggcacgcaa ccaggattgg cacgacgaga cgaggctctt
      901 caataccgat ctgaaaaaag taccacgcag cgcgcacatg cggttctatg tggggaatca
      961 tatttcagcg gaagactatc taaccacact tccggactcg gcagcaatca aggccgcgtt
     1021 agtcgaatcg atcgcgcagc tcgacaccgc catttcgatc tgggaaccgt acggcgacgc
     1081 ctggcaacgc cgcgcatata acaagtacgt gctgaacaat tatcctgcag ccgagcagga
     1141 ttttatccgg gcgctcgaga tcaaccccac caacccggtg gcgcacaaca actacggcaa
     1201 tctcctgttc aacacgcgga ggtatgatct ggctctgcaa catttcgaac aggcggtcaa
     1261 atacaatccc agatattcgc acgcgtggaa caacctggcc agcgtttacg gtgtctatgg
     1321 tgaaggggcc cgcgagcagg cgatgaacaa tcctgccgaa cgcgaccgct acctggcgga
     1381 tgccagacaa aagtgggaaa cagcgatcgg ctttttcaag aaagcgatcg agcaagaggc
     1441 ggattatgcg gtaccctacc gcatgttggg tttcacgtat cggaacctgg gcgatgaagc
     1501 catggcaaac aagtatttcc gggaggctga tgaaatagcc aagaagaagg ccaatgctaa
     1561 aaattgacat ccacacgcat atcctgcctt cggaactccc cgactttcgc aaaaagttcg
     1621 gttatggcgg cttcgtttct ctggagcatc ataagccctg ttgcgccagg atgatgatcg
     1681 acggaaaatt ctttcgcgag atccaggaca actgttggga tcctgaagtc cgcatgcgcg
     1741 agtgcgccca tcaccaggtc ggcgtgcagg tgttgtcgac cattccggtc atgttcagtt
     1801 attgggcgca accgcaggac gcgctcgacc tttccatgtt cctgaacgat cacatcgcgg
     1861 atatcgtgca gcgctatccg aagcgtttca tcggccttgg aaccatcccg ctgcaagcac
     1921 cggagcttgc gattcgggag ctggagcgtt gtatgaagat cgggttggca ggagttcaga
     1981 tcggctcgca tatcaacgac tggaacctca acgagccggc cttgtttccg gtctttgaag
     2041 cggcgcagga actgggtgcc gcgatcttcg tgcatccctg ggacatgatg ggcaaggaga
     2101 agatgaacaa gtactggctg ccctggctcg tcggcatgcc ggcggaaaca tccctggcca
     2161 tttgctccat gatcttcggc gggatctttg aacgcctgcc acgcctgcgc gttgcctttg
     2221 cgcacggcgg cggatcgttc cccgcgacct ttggcaggat cgagcatggc tttcgtgttc
     2281 gtcccgatct ggtagcggtg gacaatcctg aatctccgcg caagtacctg aaacaattct
     2341 ggctggactc actcgttcac gacccggtga tgctggatta tcttgtccgt ttcatggggc
     2401 cggagcggat cgcgcttgga accgattatc cctttccgct cggtgaattg gagcccggta
     2461 aattgatcgg cgagatgcct tattccaacg acatcaaaga acggctgctg catggctcgg
     2521 ccttggaatg gctcggattg gaaaaacgcc tgtttgcata gtacacttac agtagtttta
     2581 tttttagcac acggatgaca cggattgtac ggatgctcac ggtttttttt ctgttgaacc
     2641 tggccgcgac agcccagaca agttcgttca ggtccggatt cgcggtattg gaattgttta
     2701 catccgagag cgatgccaac agcccggcag gagatgcggt ctttcaggaa gtgatccgtg
     2761 aagcccgaaa ggcgaaacaa ccggtcatcg gccttgcctt tcacgtcgat tactggaaca
     2821 agggaagttg gaaggatccc ttcagccgca acgcctttac tttccggcag gagaattatt
     2881 ctcgggcatt cccgcagaag gaattgtaca ccccgcagtt gattgtcaat ggccgcgccc
     2941 agatgacagc ctcgtcacgc gattccgtca aagccgccat caagaaagcg ctgatcagtt
     3001 ccggcgatcc cggatttcga ctgcaagtgg attccgtatt gcgggatacg ctattcctca
     3061 cggctattac gccgaagccc ggagtgaatt gggtggtacg ggtcgcagtt acggaagacg
     3121 gactgaaaag cgaagtaaaa gccggtccca acaaagggaa gatcctggaa cacgatgccg
     3181 tggtcaggct ctggtattcc accggtgcga agtcgaaatc acttgcggtg aaggtgcctt
     3241 tgaaaggact gaagctgaac gatcggatgc gcctggtagg attgctgcag gagaagaaaa
     3301 cgatgaaaat tctgggggcg agcgagtcgg gattcgactg actaatcgat gcgttgaagt
     3361 caaaaaaaaa ttgctgaaca gccggcggac accccctctg caaataaggt ccgttacgtc
     3421 tgaacagcaa ttttatcttt tgagttggaa tcactccctg caggcggcaa aaccgacagg
     3481 gagtttttcc tatttacacc gctataatgt ctgtttgaat gcttgtgcta aataccaatc
     3541 caattctgta agtaatctga atagctcggc gaatttggat ttttttctga ctcagcgcaa
     3601 gactttcggt gaattcacaa aaaactgcgg cgagctttta tgaaatcttt ttcatgttta
     3661 gagggtgaat acgcgcgaca cgttgaagcc aagtcggatt cccgcgtcgt tccattgggt
     3721 atccgtatag ggcaggaatt gattttcaac gaggccgaat gaattggtca ggtgaacctg
     3781 gaatacgtgt ccgccggttt cgatctcgta tcctacaccg aagctgtcgt agtaggtggt
     3841 gttcgagtag gtgttggcgc gatacgcgta ttcagccgtg atcgccgagc gcttgctgaa
     3901 cttcaagcgg aagagtgctg atgctccgta ggagtcgttc tgctcgctga tgctggtgac
     3961 catgttgtaa tggttgtacc agggagcgat ctgaaagctg aagctcggcg agaacttccg
     4021 tgcgatcagg atctggtagt tgaaggacca gcgactggtt tccttctcgt
//
LOCUS       DAOTON010000144         4066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17762_length_4066_cov_4.020942, whole genome shotgun sequence.
ACCESSION   DAOTON010000144 DAOTON010000000
VERSION     DAOTON010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17762_length_4066_cov_4.020942"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..1108
                     /locus_tag="P5292_03990"
     CDS             338..1108
                     /locus_tag="P5292_03990"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HRS38320.1"
                     /translation="MKHSPRILVWALSLLLTIPAFGQTEVVPLVGKNVVKFNLTSTAL
                     SHYAIQYEYVTNPRQSVGFGFGISPDVALPFKDNLLDQFGDDDDARRAIESTRFTKYT
                     FTPEYRFYVGKKSAPEGFYVATFMRYTHMSMSQVYSFTTSAGKLHTPVVDGTFNGFGA
                     GAMIGTQWLFGKTKNLALDLWIVGPFAGIMKAKFHGTDDMSDMTQQDKIDLKEDIESI
                     EIPLWTTEATIGNNEIDVDMNGPFYGVRMLGLSFGVRF"
     gene            complement(1183..1464)
                     /locus_tag="P5292_03995"
                     /pseudo
     CDS             complement(1183..1464)
                     /locus_tag="P5292_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    1755..1854
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2024..2716)
                     /gene="purQ"
                     /locus_tag="P5292_04000"
     CDS             complement(2024..2716)
                     /gene="purQ"
                     /locus_tag="P5292_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRS38321.1"
                     /translation="MKFGVVVFPGSNCDEDMVYVLRQRMKQPVVKLWHKDHDLQNCDF
                     IVLPGGFSYGDYLRSGAIARFSPIMQEVIAFANKGGFVFGVCNGFQILCEAGLVPGAL
                     LRNDHSRFNCENVYVRPEHQAARLTAATKPGQALKIPIAHGEGRYYTDAETLRSMEAN
                     GQILFRYCDASGQVTPEANPNGSLGNIAGVCNAGKNVFGMMPHPERAADPELGNTDGQ
                     VLFEGLLQLVSA"
     gene            complement(2780..3310)
                     /locus_tag="P5292_04005"
     CDS             complement(2780..3310)
                     /locus_tag="P5292_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRS38322.1"
                     /translation="METNQEFRKQVTDETRPLKAYAMKLTQDSEEANDLVQDTVLKAL
                     TNEEKFAAGTNLKGWLYTIMKNIFINKYRRAMKSNIFNDDTDNQYYINNLATAKRNDG
                     EGNMVMREIRTAMAGLNDNLRTPFLMSYSGYKYEEIAQRLQIPLGTVKVRIHNARKEL
                     MKKLSVYKPADARREA"
     gene            3530..>4066
                     /gene="folE"
                     /locus_tag="P5292_04010"
     CDS             3530..>4066
                     /gene="folE"
                     /locus_tag="P5292_04010"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRS38323.1"
                     /translation="MKKEIIENQALPSIDDLLGGSTGSLTIHPVRSEAGDLTEEEKIQ
                     RIAARFRDIMSTLGLDLTDESLRDTPMRVAKMYVQEIFSGLNPANKPSPTLFDNAYRY
                     NQMLVERDIKVQSTCEHHFVPILGHAHVAYIAKGKVIGLSKLNRIVDHFARRPQVQER
                     LTVQIAEELKRVLDTEDVA"
BASE COUNT          979 a         1067 c         1041 g          879 t
ORIGIN      
        1 ccgctacccg ggagatttcc attccgctca acggactggc tgccggcgcc tacctgctgc
       61 aagtgcggag cgaccgtggc gaacaatcca tccgcttcac cgtgaaataa aacaagactt
      121 cttttttgtg tacgacgccc ggataaccgg gcgtcgtttt tttttgctgc atacgtattc
      181 atgcagtcaa ttggcggact tctatatccc gtttgcccac ttttcccgct ttatctgcca
      241 aatatcattg acctgcggaa tcgttcgggg tacatttaat aagagaggca agtccttagg
      301 gcttttgcca agcgtatgaa ccattgacat caccatcatg aaacactcac cacgcattct
      361 ggtatgggcg ctttccctgc tgctcaccat cccggccttt gggcaaaccg aggtcgttcc
      421 cttggtcggg aaaaacgttg tcaagtttaa cctcacctcg acggcgctca gtcactacgc
      481 gattcaatac gaatacgtca ccaacccgcg gcaatccgta gggttcggat tcggcatctc
      541 gccggatgtc gcgttaccgt tcaaggacaa cctgctggat caattcggcg acgacgacga
      601 cgcgcgtcgc gccatcgagt ccacgcgatt caccaagtac accttcaccc cggagtatcg
      661 cttctatgtc ggtaaaaaat ccgcaccgga aggattctat gtggcaacct ttatgcgcta
      721 tacgcacatg tcgatgtcgc aggtctactc cttcacaacc agcgccggaa agctccatac
      781 accggtggta gatggcacct tcaacggctt tggtgctggt gccatgatcg gtacacaatg
      841 gctgttcgga aaaacgaaga acctcgcgct cgatctctgg attgtcggac cctttgccgg
      901 gatcatgaaa gccaagttcc acggcaccga cgacatgagc gatatgaccc aacaagacaa
      961 gatcgacctg aaagaagata ttgaatcgat tgaaattccg ttatggacga cggaggcgac
     1021 aatcggcaac aacgaaatcg atgtggatat gaacgggcca ttctatggcg ttcgcatgct
     1081 gggtctcagc tttggcgttc gcttctgatc gatcttccca tcacgaaacg caaaacgcgg
     1141 cgcccggatc agggccgccg cgttttactt tctgataagt actcaagcag cagaggtctg
     1201 cgtccctgcc tttccgccaa gccaggccat cgggagatag gcgccgacaa ggtccgtgat
     1261 ggaaaaccag accggtgaag gtaacatcat ggtattggcg attcctccca acaggaagaa
     1321 tccgccgaca atgaatgccg aacgcatttt actcaccgat aaccgcgccg cgagaaacgc
     1381 gccggccagg gtgccgatcg cgtgagcgag aaatgggaag acaaagtgct tgggttccat
     1441 caggtgcatc gaggctttga ggccgaggaa aatagatgcg aggtgcgagg tgcgaaaatc
     1501 taccgcatac tgccgactga aaaaagggac gaggagcgag tccgaggaaa atactgccta
     1561 ctgccaactg ccaactgaaa aaagggacgg gggacgagat gcgaggtgcg aggtgcgagg
     1621 tgcgaggtgc gaggtatgaa aaactaccgc atactgccta ctgaaaaaag ggatgagaag
     1681 cgagaccgat gaaaatactg cctaccgcct actgccaact gcctactgaa aaaagggacg
     1741 agggacgagg aaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaccga
     1861 gaccgaggaa aatactgcct actgaaaaaa gggacgggag acgagatgcg aggtgcgagg
     1921 catgaaaaac taccgcatac tgcctactga aaaaagggac gagaagcgag accggggaaa
     1981 ataccgccta ccgccaactg tctaccgtca accgaattcc agctcaggcg gagaccagtt
     2041 gcaacaatcc ttcaaaaaga acctgtccgt cagtgttgcc caactccgga tcggcggcgc
     2101 gttcgggatg gggcatcata ccgaagacgt tcttgccggc attgcacaca ccggcgatgt
     2161 tgccgaggga tccgttggga tttgcttcgg gtgtcacttg tccggaagca tcgcagtagc
     2221 ggaacaggat ctgtccgttt gcttccatgg aacgcaatgt ttcggcgtcg gtgtaatagc
     2281 gtccttctcc gtgtgcgatc gggatcttga gcgcttgtcc gggcttggtc gcagccgtca
     2341 gtcgcgcagc ctgatgctcg ggacgaacat acacgttctc gcagttgaag cgcgaatggt
     2401 cgttgcgcaa caatgccccg ggtaccaggc ctgcttcgca gaggatctgg aacccgttgc
     2461 agacaccgaa gacaaagccg cccttgttgg caaaggcaat gacttcctgc atgatcggag
     2521 aaaagcgcgc gatggcgccg gagcgcaggt agtcgccgta gctgaatccg cccggaagta
     2581 cgatgaaatc gcagttctgc aaatcatgat ccttgtgcca caacttcacc accggttgct
     2641 tcatccgctg gcgcagcaca tagaccatgt cttcgtcaca gttcgatcca gggaaaacga
     2701 cgactccgaa tttcatgacc cgaaggtacg aaaaaacccc tgtgcagaca ggggtttcag
     2761 gtcatgagcc ggatgcgctt caggcttcgc gacgcgcgtc tgcaggcttg taaacgctga
     2821 gtttcttcat caattccttc cgggcattgt gaatgcgcac tttcaccgta ccgagcggaa
     2881 tctgcaaccg ctgcgcgatc tcctcgtact tgtagccgct gtagctcatc aggaagggcg
     2941 tccgcaggtt gtcgttcaaa ccggccatgg cggtgcggat ctcccgcatg accatgttcc
     3001 cttccccgtc gttccgtttt gcggtcgcga ggttgttgat gtagtactga ttgtccgtat
     3061 cgtcgttgaa gatgttcgac ttcatcgcgc gacggtactt gttgatgaaa atgtttttca
     3121 tgatggtgta caaccatccc ttcaggttcg taccggcggc gaacttctct tcgttcgtca
     3181 aggctttcag gacggtatcc tgcaccagat cattcgcctc ctcgctatcc tgagtgagtt
     3241 tcatcgcata tgctttcaac ggacgggtct cgtcagttac ctgcttgcgg aattcctgat
     3301 tcgtttccat ggcgatgtat tttgaactat tctaaactgt ttaactatct gtgcctaatc
     3361 gttaaacaaa aatagaataa aaatggtcaa cttcgacatt cgtgtttaat atttaactct
     3421 tattaactaa acaaaacttg gatttttcca tgaaattcta tggccgaaaa caatctgaaa
     3481 acggccttgt tctctctatt cctccggcag tactggggta aaatcaccta tgaagaaaga
     3541 aataattgaa aatcaagctc ttccatccat cgacgatctg ctgggagggt ccacaggatc
     3601 gctgacaatt catccggtcc gatccgaggc gggggatctg accgaggagg agaagatcca
     3661 gcggatcgct gcgcggttcc gtgatatcat gtcgacactc ggacttgacc ttaccgatga
     3721 aagtctgcgt gacacgccga tgcgcgtcgc caagatgtat gtacaggaaa ttttcagtgg
     3781 cctcaatcca gccaacaagc ccagccctac gcttttcgac aacgcctatc gctacaacca
     3841 gatgttggtg gaacgcgaca tcaaggtgca atcaacctgc gaacaccact tcgtaccgat
     3901 cctgggacat gcacacgtag cctacatcgc gaaaggaaaa gtgatcggac tttcgaaact
     3961 caaccgcatt gtcgatcatt tcgcgcgccg tccccaggtg caggagcgac tgaccgttca
     4021 gatcgcggaa gaactgaagc gggtactcga cacggaagat gtcgcc
//
LOCUS       DAOTON010000145         4061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17795_length_4061_cov_2.702696, whole genome shotgun sequence.
ACCESSION   DAOTON010000145 DAOTON010000000
VERSION     DAOTON010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17795_length_4061_cov_2.702696"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..1824
                     /locus_tag="P5292_04015"
     CDS             334..1824
                     /locus_tag="P5292_04015"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HRS38324.1"
                     /translation="MSLKNALLLAFFGLLIGFSSCKKDEFITDGDAKLEFSTDTVIFD
                     TVFTTVGSSTEVFVVYNRNDQPVKVSSIRLATGDQSMFRLNVDGLPGRSFSDVEIGAD
                     DSIFIFVEVTVDPNNQNTPLVVTDSILFETNGNLQDIDLVAWGQDAYFHKPPAGSPPL
                     FFLDCNDTWNNDKPHVIYGYALVDSACTLTINAGTNVHLHPGSGIIVLSSGTLKVEGT
                     TGSPVTIQGDRLGFDYQEIAGQWDRIWLSNITRSNLINGTNEIGPGSRNSVIRNCVIK
                     NGNIGLHVDTVFGAGETTLRLENTFVRNMSGNALLLRGAVIDSYNCAFANCGLQVANM
                     LYGGTYRWYHCTFANFWSEESRQDPVVLMNNYFGNNVRRIDANYYNCIVYGNNDQELG
                     IDSFPNAAPGLFNYKFDHALLKIEGTFPTSNPLFYNAIIRATGSSNSPRFQDVDNNIY
                     KLDSTSIAIDAGDAAFLSIDPILNTDLEGLVRPQGLGPDLGAYERR"
     gene            2073..2684
                     /locus_tag="P5292_04020"
     CDS             2073..2684
                     /locus_tag="P5292_04020"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HRS38325.1"
                     /translation="MLLPTAYLPPISWLKQALSGEAVVIEAKEHFSKQTWRNRCRIYG
                     GNGPIELVIPVDHTNRWRIPIDELKTSTATDWKSLHWKSIRSAYGKAPYFEYYAETIE
                     ACFRKDAPEHLLDFNEACLRSVLKLLKADIQIERTESFTPYTDNDPRFVWSEIDGETG
                     WTFPSYYQSFADRHGFLPNLSSLDLLFHLGPDSREYLEKITRA"
     gene            complement(2694..3677)
                     /gene="pfkA"
                     /locus_tag="P5292_04025"
     CDS             complement(2694..3677)
                     /gene="pfkA"
                     /locus_tag="P5292_04025"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRS38326.1"
                     /translation="MASNIKCIGVLTSGGDAPGMNAAIRAVTRAAIFNGIRVKGIYKG
                     YRGLISGDVVEFKTDSVSNIIQRGGTILKTARSEQFKTVEGRAKAYENLRTNGVDGLI
                     LLGGDGSIRGIQQFLQEHDLPVVAIPKTIDNDIYGTDYAIGYDTALNTVVQAIDKIRD
                     TADSHDRIFFVEVMGRDAGMIAVLSGIGSGAEAIAIPETRTRIEQIVQVLQRGWHRKK
                     TSMIVIVAEGDDAGGAYKIADAVRQRFDSFDTRVSVLGHMQRGGSPTCFDRVLSSRLG
                     VAAVDALVQGRRDIMVGVIDNKIAETPFETVVARRKEFPYDLMRVAEILSL"
     gene            complement(3770..>4061)
                     /locus_tag="P5292_04030"
     CDS             complement(3770..>4061)
                     /locus_tag="P5292_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRS38327.1"
                     /translation="YHDTICRQVSNRIPHINMFASQYDIIFFIAGEKSSNGKVLFNEC
                     KKSNPNSYFIHSPDELDVSLIKDNDRVGICGATSTPKWQMQALAERIMKLKP"
BASE COUNT          984 a         1146 c          967 g          964 t
ORIGIN      
        1 agtgtggaaa atcttacgcg tccaagaaag gccattgggg tagcgggctg gagattagta
       61 aatagtaatt agtaactaga aactggtaac tagaaactag aaactagaaa cttgttggaa
      121 gtccctaatt actaattact agttactaat taccagttac aaatcactcg aacgccacca
      181 cacaaccttc caacatacaa catacaacat acaaccttca accttcaacc ttcaaccaac
      241 ccgaaacgcg aaactcgaaa cccggaacaa caaacaatct tacccatcca atccccacaa
      301 cccaccccca cctttcccgt atatttgccg cctatgagcc ttaaaaacgc gttgttactg
      361 gcttttttcg gattgctgat cggattttct tcctgtaaaa aggacgaatt catcaccgac
      421 ggcgacgcga agctggagtt ttcgactgat acggtcatct tcgatacggt cttcaccacg
      481 gtcggcagtt ccacggaggt tttcgtcgtt tacaaccgca acgaccagcc ggtgaaggtc
      541 agttccatcc gcctggctac cggcgaccag tcgatgttcc gcctgaacgt cgatggcctg
      601 cccggacgct cgttcagcga tgtggagatc ggcgccgacg acagcatctt cattttcgtg
      661 gaagtaacgg tggacccgaa caaccagaac acgcctttgg ttgtcacgga ttccattctc
      721 ttcgagacca acggtaacct gcaggacatt gatctggtgg cctggggaca ggatgcttat
      781 ttccacaagc cgccggccgg ctctcccccg ctcttctttc tggattgtaa cgatacctgg
      841 aacaacgata agccgcatgt catctatggc tacgccctgg tcgattccgc ctgcacgctg
      901 acgatcaacg cgggaacgaa tgtccactta catcccggct cgggcatcat cgtcttatcc
      961 tccgggacac tcaaggtgga aggcacgacc ggcagtccgg tcaccatcca gggcgaccgg
     1021 ctcggcttcg actaccagga gatcgccgga cagtgggacc gcatctggct gagcaacatc
     1081 acacgcagca acctgatcaa cggcacgaac gagatcggtc cgggttctcg taattccgtt
     1141 attcgaaact gcgtgatcaa gaacggcaac atcggcctgc acgtcgatac cgtattcggt
     1201 gcgggcgaaa cgacgcttcg cctggaaaac accttcgtgc ggaacatgag cggcaacgcg
     1261 ctcctgctgc gcggggcggt gatcgattcg tacaactgcg cctttgccaa ctgcggactg
     1321 caggtggcca acatgctcta cggcggcacc tatcgctggt accactgtac ctttgcaaac
     1381 ttctggtcgg aggaatcccg ccaggacccg gtcgtgttga tgaacaacta cttcggcaac
     1441 aacgtgcgac ggatcgacgc gaactattac aactgcatcg tgtacggcaa caacgaccag
     1501 gaactgggca tcgactcctt tcccaatgcc gcccccggcc tgttcaacta taagttcgac
     1561 cacgcgctgc tgaagatcga aggcaccttc cccacttcca acccgctctt ctacaacgcg
     1621 atcatccgcg ctacgggcag cagcaacagt ccgcgcttcc aggatgtaga caacaatatc
     1681 tacaaactcg acagtaccag catcgccatc gatgccggtg acgccgcttt tctttccatt
     1741 gacccgatcc tcaacaccga tctcgaaggc cttgtcaggc cgcagggttt gggaccggat
     1801 ttgggggctt atgaacgccg atgatttctt aattgtagat tgaaaattga aggtagaagg
     1861 tagaaggaat cacgctgctt atgtacatgg agactgttga agttggcctg tgtcagtgat
     1921 ccaacaatca tatggtataa ttcgtcacct atatcaaaat ctcaagattc tcttactcac
     1981 ttcgcaatca ttcgcacttt caaacgccca tttcaatcaa ctttcaactt tcaactttca
     2041 accttcaacc ttcaactttc aactaaaaag taatgctgct cccaaccgcc tacctccctc
     2101 ccatctcctg gctgaaacaa gccttgtccg gagaagcggt tgtgatcgaa gcgaaagagc
     2161 atttttccaa acagacctgg cgcaaccgtt gcaggatcta tggcggcaac ggtccgatcg
     2221 agttggtgat tccggtggac cataccaacc gctggaggat cccgatcgac gaactgaaaa
     2281 cttccaccgc caccgattgg aaaagcctgc actggaagag tatccgttcg gcttacggca
     2341 aggcgcccta cttcgagtat tatgcagaga cgatcgaagc ctgcttccga aaagacgcac
     2401 ccgaacacct gctggatttc aatgaagcct gtctgcggag cgtcctgaaa ctgttgaaag
     2461 cggatatcca gatcgaacgc acggaatcgt tcacgccata cacggacaac gacccgcgat
     2521 ttgtatggtc ggagattgat ggggagaccg gatggacgtt tccttcctac taccagtcgt
     2581 tcgccgaccg tcacggcttt ttacctaacc ttagttcgct ggacctgctt tttcatctcg
     2641 ggccggatag ccgggaatac ctggagaaaa tcacccgcgc ttaaccattc tcctcagagt
     2701 gacaggatct ccgcgacccg catgaggtcg taggggaatt ctttgcggcg cgcaacgacc
     2761 gtttcaaagg gagtttccgc gatcttattg tcgattacac cgaccatgat gtcgcgtcgg
     2821 ccctgcacca gcgcgtcgac cgcggctact cccagccgac tggagagcac ccggtcgaag
     2881 caggtcggac ttccgccccg ttgcatatgt cccaatacgc tgacgcgcgt atcgaaggaa
     2941 tcgaaacgct gtcgcacggc gtccgcgatc ttgtatgctc cgccggcatc atcgccttcg
     3001 gcaacgatca cgatcatgga tgtcttctta cgatgccagc cgcgttgcag gacctggacg
     3061 atctgttcga tgcgtgtacg cgtttccgga atggcaatcg cttccgcccc ggagccgatg
     3121 ccgctgagca cggcgatcat gccggcatca cgtcccatca cttccacgaa gaaaatccgg
     3181 tcgtgcgaat cggccgtgtc gcggatcttg tcgatcgcct gcacgacggt gttcaatgcc
     3241 gtatcgtaac cgatcgcgta atcggttccg tagatgtcgt tatcgatcgt cttcggtatg
     3301 gcgacgacgg gaagatcgtg ctcctgtaaa aactgttgaa tgccgcgaat ggagccatcg
     3361 ccacccagga ggatcaatcc atcgacaccg ttggtccgca ggttctcata cgcctttgcc
     3421 cgtccttcaa cggtcttgaa ttgttcggaa cgcgccgtct tcaggatcgt accgccacgc
     3481 tggatgatgt tgctcacgct atctgtttta aattccacta catctccgct aataagacct
     3541 ctatatcctt tatagatacc ttttacccga attccgttaa aaatggcagc gcgggttact
     3601 gcacgaatag cagcattcat acccggagca tcgcctcctg aggtaagaac tcctatacat
     3661 ttaatgtttg atgccataat gagaaattat tttttaggat acccttatgt attttcccaa
     3721 aacaatgaac tgcaaagata aagttttttg cgggtaacaa aaaagctttt tagggtttta
     3781 atttcatgat tctttctgcc agcgcttgca tttgccactt aggagtggat gttgcgccgc
     3841 agatgccaac cctgtcattg tcttttatca acgaaacatc caattcgtcg ggagaatgga
     3901 tgaagtagga gttgggatta ctctttttgc attcattaaa aaggactttt ccgttggagc
     3961 ttttttctcc ggcaataaag aagatgatat cgtattgcga tgcgaacatg ttgatgtgcg
     4021 gaatccggtt ggatacctgg cgacagatcg tatcgtgata t
//
LOCUS       DAOTON010000146         4060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17797_length_4060_cov_3.834707, whole genome shotgun sequence.
ACCESSION   DAOTON010000146 DAOTON010000000
VERSION     DAOTON010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17797_length_4060_cov_3.834707"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="P5292_04035"
     CDS             <1..226
                     /locus_tag="P5292_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRS38328.1"
                     /translation="RLRALYDELKVHTIEERMELWGLNADRADVIVPAARIYTGIMKA
                     AQINEIIVPQIGLADGIVHRLHEERAVMKP"
     gene            complement(299..1222)
                     /locus_tag="P5292_04040"
     CDS             complement(299..1222)
                     /locus_tag="P5292_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRS38329.1"
                     /translation="MSLVVVGTVAFDAIQTPFGKTDKIIGGAATYSGTAASYFTKNVK
                     LVSVVGDDFPPAMIDQFRKSGMSTEGLQVKHGAKTFFWSGRYHEDMNTRDTLDTQLNV
                     LLDFDPVIPDSYQDCEFLMLSNLMPQLQKKVIERMRTRPKLVVMDTMNFWMETQWDAL
                     METIQLVDVLTVNDSEARLLTKEYSLVKAAQKILQMGPRYLIIKKGEHGALLFNKEQV
                     FFAPALPLEDVFDPTGAGDTFAGGFIGHIAQTRDISFDNMKRAIIFGSAMASFCVEKF
                     GLERMIGLTQAEIDERVQQFIDLVQFDIELV"
     gene            1392..1465
                     /locus_tag="P5292_04045"
     tRNA            1392..1465
                     /locus_tag="P5292_04045"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1426..1428,aa:Asn,seq:gtt)
     gene            2154..2513
                     /locus_tag="P5292_04050"
     CDS             2154..2513
                     /locus_tag="P5292_04050"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRS38330.1"
                     /translation="MDFKGLIVYRKALEVYTQLTTRVLTIDGLDKDLKNQLRRAMMSI
                     VLNIAEGSSRFSAADRRNFLVISRGSAFECSAVLDLVAISTAKHFPDLDMLLVEVSKM
                     LYKMIDTLNEKQRSKGG"
     gene            2763..3530
                     /locus_tag="P5292_04055"
     CDS             2763..3530
                     /locus_tag="P5292_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRS38331.1"
                     /translation="MKKLSRLFVPLVVLISIFSSCTPYRNILMFQPTEQDTTLKALDT
                     TYRPIIGPNDILDIFVTSTSPEASKYFNYSEQPDNQASMVSGYLVDSKGEIQIPFIGS
                     LRVAGLHTDAARDTIRHRLEKYLVSPSVKLSLRNFRVTVIGEVTHPGMFNVQNERVTL
                     PEALAMAGDLTVFSVRDQVLVIRDSAGYKTYNYVDLYSRELFSSPYFVLHSNDIVYVQ
                     PNKKRRFQGENYYRVFPVVFGTITLFLTALQLVNQSK"
     gene            3632..4006
                     /locus_tag="P5292_04060"
     CDS             3632..4006
                     /locus_tag="P5292_04060"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HRS38332.1"
                     /translation="MDVYYDDGCSFCKRAMRIARRLDIFHRLRFLPVSAQLGKAGPLQ
                     HIPAQDLLYDIHGIDDKGRIKRGIAVYQSALRAMIWLAPLGWLLMIPGLSHLGVAIYR
                     RIADNRYGSSCAVDPEEISRGS"
BASE COUNT          931 a          984 c         1112 g         1033 t
ORIGIN      
        1 acgactgcgc gcgttatatg acgagttgaa agtgcacacc atcgaagagc gtatggagct
       61 gtgggggttg aatgcggatc gtgccgacgt aatcgttccg gcggcgagga tctataccgg
      121 catcatgaaa gcggcgcaaa tcaatgagat catcgtaccg cagatcggac tggccgacgg
      181 gatcgtgcat cggttgcacg aagaacgcgc ggtaatgaaa ccctgatccc cgatcagacg
      241 atgaaacaaa aaagccggag tacaaacgct ccggcttttg gattttatca atcgggactt
      301 acaccaactc gatatcgaac tgcaccaggt cgatgaattg ttgtacgcgt tcgtcgatct
      361 cagcctgggt gaggccgatc atgcgctcga gtccgaattt ctcgacgcag aacgaagcca
      421 tggcggagcc gaagatgatg gcgcgtttca tgttgtcgaa ggagatatcg cgcgtttgcg
      481 cgatgtgacc gatgaagccg ccggcgaagg tgtcgcccgc gccggtcgga tcgaacacat
      541 cttcgagcgg aagggcggga gcgaagaaca cctgctcctt gttgaagagg agtgcaccgt
      601 gttcgccttt cttgatgatc aggtaacgcg gacccatctg gaggatcttc tgcgcggcct
      661 tcacgagcga gtattctttg gtgagcagcc gggcttcgct gtcgttgacg gtgaggacat
      721 cgacgagttg gatggtttcc atgagcgcgt cccactgcgt ttccatccag aagttcatcg
      781 tgtccatgac gaccagcttg gggcgggtgc gcatgcgctc gatgactttc ttctgcagtt
      841 ggggcatgag gttgctgagc atgaggaact cgcaatcctg gtaagaatcg ggaatgaccg
      901 ggtcgaagtc gaggagcacg ttcaactggg tgtcgagcgt gtcgcgggtg ttcatatcct
      961 cgtggtagcg gcctgaccag aagaacgtct tggcgccatg cttgacctgg aggccttcgg
     1021 tggacatgcc gcttttgcgg aactggtcga tcatggccgg ggggaaatcg tcgcccacga
     1081 cggagaccag cttgacattt ttggtaaaat aggaagccgc tgtacctgaa taagttgcag
     1141 cgccgccgat gattttgtct gtctttccga agggagtttg gatggcatca aaggccaccg
     1201 taccgacgac taccaggctc atttaggcta attttttggc gaatatattg ctttttccct
     1261 gaaaagcctt acttttgtcg ccccttagta cccctgatct gcgtgcttta gtcctgttgt
     1321 tcaacacaat aggatggctg accgtgaaga ggaagggttc tccgcccgga gcgttccgcg
     1381 cgaaggatta ttcctcctta gctcagctgg ttagagcatc tgactgttaa tcagagggtc
     1441 cctggttcga gcccaggagg gggagcttat agttcgaatg cgagaaacag tgtgggtgaa
     1501 cagtatttga ctgttcactc gcactgtttc tgttttgtcg cactactatc attcgtggga
     1561 ttcttggaaa gattagtact gttaatcaga gccccgatag ctatcggggc tggttcgagc
     1621 ccaggagggg gagcatatag ttcgaatgcg agaaacagtg tgggtgggca gtgtttgact
     1681 gttcactcac actgtttctc acacttgcac ttctgagatt agtgtaagag taagcaacag
     1741 tgtgagtgag cagtgtttta ccatccaccc gcactgtttc tgttttgtag cacgtctgag
     1801 aatagtgtga gtgtgagcaa cagtgtgggt gggcagtgtt tgactgttca ctcacactgt
     1861 ttcttacact tgcacttctg agattagtgt aagagtaagc aacagagcga gtgagcagtg
     1921 ttttactgtc cacccgcact gtttctgttt tgtcgcactt ctgaaagtag tgtatgtgta
     1981 agaaacagtg tgagtaaaca gtgaatgatt accctcccga actgtttctg ttttattgta
     2041 cttctgaaaa tagtgtaagt gtaagaaaca gtgcgagtga acagtggaat tataacctat
     2101 aatcccgcat atattgtttg tataccggct ggagcttggg atagttttgc ggtatggatt
     2161 tcaaaggact gatcgtttat cgaaaagcac tggaggtgta tacccaacta acaacccgcg
     2221 tactgacgat cgacggactt gataaggacc tgaagaacca gcttcgacgc gccatgatga
     2281 gcatcgtact caatatcgcg gagggaagta gtcgtttcag cgcggcagac aggaggaact
     2341 ttttggtgat ttccagagga tcggcttttg aatgttctgc agtgctcgac ctggtagcca
     2401 tctccaccgc caaacacttc cccgaccttg atatgctgct ggtcgaggtc tcaaagatgt
     2461 tatacaaaat gatcgacacg ctgaatgaga agcagcgaag taagggtggg tgatttgcat
     2521 ttgactgttc actcacactg tttcttacac tcacacttct gaaaatagtg taagtgtaag
     2581 aaacagtgtg agtgagcagt gtttgattat ccacccgccc tgtttctgtt ttgtcgcact
     2641 cctgaaaata gcgtaagtgt gagaatcagt ccaagtgaac cgtgtttttt gagctggcgc
     2701 gaaacttttt ttggggctgg aagtaaacac ccattgccct atgctacggg cgcgctatgt
     2761 ccatgaaaaa gctgtcgcgg ctcttcgttc cgctggtcgt actgatttcg atcttctcct
     2821 cctgcacgcc gtaccggaat atcctgatgt tccagcccac cgagcaggat acgacactca
     2881 aagcgcttga caccacctac cggccgatta tcggacctaa cgacattctc gacattttcg
     2941 tcaccagtac cagtccggag gcgagtaagt acttcaacta ttccgagcaa cccgacaacc
     3001 aggcgtccat ggtcagcggc tacctggtcg attcgaaagg cgagatacaa atacccttca
     3061 tcggctcttt acgggttgcc ggtttacata cggatgctgc ccgcgacacc atccggcacc
     3121 ggctggagaa gtacctggtc agcccgtcgg tcaaactaag cctgcgcaac tttcgggtga
     3181 cggtgatcgg cgaagtaacc catccgggaa tgttcaatgt gcagaacgaa cgtgtcaccc
     3241 tgcccgaagc cctggccatg gccggggacc tgaccgtgtt tagtgtccgg gatcaagtgc
     3301 tggtgatccg cgacagcgca ggatacaaga cctataatta tgtagacctg tattcacgag
     3361 aactgttttc ttcaccgtat ttcgtgctgc attcgaacga catcgtctat gtgcagccca
     3421 ataagaaacg ccgcttccag ggtgagaatt actaccgtgt cttcccggtg gtattcggaa
     3481 cgatcacctt gttcctgaca gccctgcaac tggtgaacca gtcgaaataa acgggcgatc
     3541 cacagtcggg ttaaacaatc cgactcccgg caggttgtta tagtttggaa aagagtcgcg
     3601 tcatttctcc gacagcgact cccggaattc gatggacgtc tattacgatg atggttgttc
     3661 gttttgcaag cgcgcgatgc gtattgctcg ccgtttggac atctttcatc gccttcgttt
     3721 tctgccggta agtgcacagc tcgggaaagc cgggccattg cagcacattc cggcgcagga
     3781 tttactctat gatatccatg gcattgatga caagggacgc atcaaacgag ggatagccgt
     3841 ttaccagtcg gctttgcgtg ccatgatctg gttggcccct ttaggttggt tactcatgat
     3901 tcccggcctt tctcaccttg gagtcgcgat ttatcgccga attgcggata atcggtacgg
     3961 atcctcctgc gcggttgatc cggaagaaat atcgaggggc tcatgatcca accaaaatat
     4021 ccggtattct cgcgccagaa acgcccgcac tgtccggtat
//
LOCUS       DAOTON010000147         4055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17824_length_4055_cov_3.312500, whole genome shotgun sequence.
ACCESSION   DAOTON010000147 DAOTON010000000
VERSION     DAOTON010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17824_length_4055_cov_3.312500"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="P5292_04065"
     CDS             complement(<1..1314)
                     /locus_tag="P5292_04065"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRS38333.1"
                     /translation="MLLGLKRLSLKCAWIISLLFVFIDAFGQREEIDSLKKTLATQLA
                     DTDRVRTMIRLSYQYINRHELELPMALADSALRFADQIGFKVGYAEALMLKGSVYRWT
                     VNEAKGLEYYYQALRILETAGDKRKLAVCYQLLGFSNFWQQNHEAAFDAYRKCLKLSR
                     EAGYTDGIIESLAYLCNERFDKRDLEGALALSTEIEELSDTTGNNRGLGYAYSGFAKY
                     WREKGGEAERTGNRPLMRECLIKSDHYEELAVEQFDQVDDPGAAGDCYNRRAEIHIKL
                     NQLGVAAQLLEKARAYAVRFGHDDNFKNYFYTLSNLDSASGDFRNAYAHYKEYITYKE
                     RLTDLENARKSQQLYAQYEFEKKEAAVAATQKLRDADAASELRMQKLLRNAFIVGTIL
                     LILLIAVLINRQKLKRSIELERMRSRLSRDLHDDIGSTLSSINMLS"
     gene            complement(1329..1961)
                     /locus_tag="P5292_04070"
     CDS             complement(1329..1961)
                     /locus_tag="P5292_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS38334.1"
                     /translation="MIRILIFDDNPDFVDGISGLVAEAEGMELVGAYKNCKNVVRNVQ
                     HHQPDVVLMDIDMPVENGLQGLRTLRDAGLEVLVLMLTVFDDNERVFQSICFGANGYL
                     LKHTPPEQIVHAIREAHTGGAPMTPSVARQVLKLFSQPFRHAKELQTLTPREHDVLSC
                     LVRGYSYKMAAAELDVSIETLRFHIKNIYVKLHVNSKSEAVAKAIQNKLT"
     gene            complement(2318..3271)
                     /locus_tag="P5292_04075"
     CDS             complement(2318..3271)
                     /locus_tag="P5292_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRS38335.1"
                     /translation="MKAFTRTVYGGPEVLRLSETDKPLVGDDQILVRVRANSANPADW
                     HILRGKPYFARLTFGLFTPKDKIPGADFAGVVEAAGRNVNDFRAGDRVFGESLKGGAF
                     AEYIAVPATVCARMPAAVDFPVMAAVPIAGLTALQAVVTHGKLKQGESVLINGSSGGV
                     GHFAVQIAKAHGARVTAVCSSRNAEFVRSLGADAVIAYDKEDIHLHTTKYDLVLDTNG
                     NLEYRDFVRMGRRGVVTGFTTMGHMMSVQFKKALGKFPLVQFTAQANPKDLATLSSLV
                     REGKIRPHIEKSYPYAELPQAIGYIEAMRTRGKVVMNWESR"
     gene            complement(3278..>4055)
                     /locus_tag="P5292_04080"
     CDS             complement(3278..>4055)
                     /locus_tag="P5292_04080"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRS38336.1"
                     /translation="KVTRDSIPVLVFNPLHKDNLPVIIQYHGGGFISPLVPGLEYSLW
                     QDARNYEAVVFAVDYRVAPEHRFPAAVDDSYASFLWISENAQRFGGDVNKIVLMGNSA
                     GANLVAAITQRAKKAGIGNRIKLQVLNGLPADLRPQHMESSESYLQNASGYFQTKALC
                     YFAVETYAPEQYNDPEVSPILAADFSGLPPALIVTAEFDPMRDDGPLYAAKLSAAGVK
                     VREKCFAGQLHCLIGALPESKAQHEFVTLVKNAMSDHLSK"
BASE COUNT          959 a         1136 c          976 g          984 t
ORIGIN      
        1 gctcaacatg ttgatgctgc tgagggtact tccgatatcg tcgtgcaggt cgcgggataa
       61 gcgggagcgc attcgttcca attcgattga acgcttcagt ttctgtctgt tgatcaagac
      121 tgcaatcagc agaatcagca ggatggtacc gacgataaag gcgttccgga gaagcttctg
      181 catccgcaat tccgaagcag cgtcggcatc tcttagtttt tgagtcgcag ctacggcagc
      241 ttccttcttc tcgaattcgt attgtgcata caactgctgg ctcttcctgg cattttcgag
      301 atcggtcaat cgctccttgt acgttatgta ctccttgtaa tgagcatagg cattccgaaa
      361 atcaccggac gcactgtcca gattcgacaa agtgtagaag taattcttaa aattatcatc
      421 atgaccgaag cgcaccgcat atgctcgcgc cttttccaat aattgggcgg ctactcccaa
      481 ttgattcaat ttgatgtgga tctctgcccg acggttgtag caatcaccgg cagctccggg
      541 atcgtccacc tgatcgaact gctcaacagc caattcctcg tagtgatccg attttatcaa
      601 acattcgcgc atcaagggcc ggttaccagt tctttccgct tctccaccct tctcgcgcca
      661 atacttcgca aatccggaat aggcgtatcc caacccgcga ttatttccgg tagtatcgga
      721 taattcttca atctcggtag agagcgccaa cgcgccttcc agatcacgct tatcgaatcg
      781 ctcgttacag agataagcaa gcgactcgat gataccgtcc gtataaccag cctcccgact
      841 tagtttcagg catttccggt aagcatcgaa ggccgcttca tggttctgct gccagaaatt
      901 actgaagccc aataactgat aacaaacagc gagcttgcgt ttatcgccgg cggtctctaa
      961 gatcctgagg gcctgataat agtactccag tcctttcgct tcattcacgg tccaacgata
     1021 gacggaccct ttcagcatca gggcttccgc atatccgacc ttgaacccga tttgatcggc
     1081 aaatctcaat gcggagtctg ccaatgccat cggaagttcc aactcgtgcc ggttgatata
     1141 ctgataactc agtcggatca tcgtccttac gcgatccgta tcagcgagct gggtggcgag
     1201 ggttttctta agtgaatcga tctcctcgcg ctgcccaaat gcgtctatga atacaaacaa
     1261 cagggaaatg atccatgcac acttcaacga taatcgtttt aagcctaaaa gcatggatac
     1321 aaagccggtc aggtcagttt gttttgaatc gcctttgcca ccgcttcact tttcgaattg
     1381 acatgcagtt taacatagat gttcttgatg tgaaagcgaa gcgtttcgat gctgacatcc
     1441 aattcagcgg cggccatttt gtagctgtag cccctcacca gacagcttag gacatcatgt
     1501 tcacgcgggg tcagggtttg caactccttg gcatgacgaa acggctgtga aaacagtttc
     1561 aagacctgtc tcgcaacgga aggggtcatg ggagctccgc cggtatgagc ttctcgaatg
     1621 gcatgcacga tctgttccgg gggggtgtgc ttgagcagat atccattggc gccgaaacag
     1681 atcgactgaa acacccgttc attgtcgtcg aataccgtca gcattagcac caggacctcc
     1741 aagccggcat cccggagtgt acgcaaaccc tgcagaccgt tctccaccgg catgtcgata
     1801 tccatcaaca ccacatcggg ctggtggtgc tgcacattac gaacaacgtt tttacaattc
     1861 ttatacgcgc ccactaattc cataccctcc gcttctgcaa caagtccact gatcccgtca
     1921 acaaaatcgg gattatcatc aaaaattaat atgcgaatca ttcctcttac aagtcattga
     1981 agatagtctt tttttcgtcc ccgggccctg ccaacttgca aattaatcac actttcaatg
     2041 gcgtttatac cacatattca ggtgggaaat gggtctggaa aaagtgtgcg cagtcaaatt
     2101 tgggaacacg cctatttacg tggaacagtg aaaataaaat gcagagagca agccgtgttt
     2161 gtactaggtc ccaaataaag cagcagggat ctatcatgcc aagggccggc gttcaattga
     2221 atcaagatct cctcaacgaa gaagttgagc cgtattgttc caatacctgg ataatagtac
     2281 catatcgatc aatagatcca ccctatccag tacacaatca tctgctctcc cagttcatca
     2341 ccaccttgcc ccgcgtacgc atcgcttcga tatagccgat cgcctgcggt aattcagcgt
     2401 agggatagga cttctcgata tgcggacgga tctttccttc ccgaaccagc gacgacaagg
     2461 tcgccaggtc tttcgggttc gcctgggcgg tgaattgcac gagcgggaac tttcccagtg
     2521 ctttcttgaa ttgcacggac atcatatgtc ccatcgtcgt gaagcctgtc acgacgcctc
     2581 gccgacccat gcgaacaaaa tccctgtact ccagattgcc attcgtgtcg agtaccagat
     2641 catacttagt agtatgtaaa tggatgtctt ctttgtcgta cgcgatcacc gcatcggccc
     2701 cgagcgagcg gacaaactcg gcattccgtg aagagcagac ggccgtcacc cgtgcgccat
     2761 gggctttcgc gatctgaacg gcaaaatgtc cgacccctcc ggaggaaccg ttgatcaaga
     2821 ccgactcgcc ttgtttcagt tttccgtggg tgacgacagc ctgcaaggcc gtcagtccgg
     2881 caatcggaac ggcagccatg acgggaaagt cgaccgcggc gggcatgcgc gcgcaaaccg
     2941 tcgcgggcac cgcgatgtat tcggcaaagg cgcctccctt cagcgattca ccgaacacgc
     3001 gatcgcccgc tctgaagtcg ttcacatttc gtccggccgc ttccaccaca ccggcaaagt
     3061 cggcacccgg aattttatcc ttgggcgtga aaagcccgaa cgtcaatcgg gcaaagtatg
     3121 gtttaccgcg caggatgtgc cagtcggccg ggttggctga attcgccctg acccggacga
     3181 ggatctggtc gtcgcctaca agaggcttat cggtttcact caggcgaagg acttccggtc
     3241 ctccataaac ggttctggta aatgctttca tgaggtttta ttttgaaagg tgatccgaca
     3301 tggcattctt aacaagtgtc acaaattcat gctgcgcctt ggattccggc agtgctccga
     3361 tcaggcagtg gagttgcccg gcgaaacact tttcccggac cttgacaccg gcagcgctca
     3421 acttagcggc atacaatgga ccgtcgtcgc gcatcgggtc aaactcggcc gtgacgatga
     3481 gcgccggcgg caatccggaa aaatccgctg ccagtatcgg tgacacttcc gggtcgttgt
     3541 actgctccgg tgcataggtc tccaccgcga aataacacaa cgccttcgtt tggaaatagc
     3601 cgcttgcatt ctgcaagtaa gattcggaag actccatgtg ctgcggacgc aaatccgccg
     3661 gcaagccgtt caacacctgt agttttattc gatttccgat tccagccttc ttcgctcgct
     3721 gtgttatcgc tgccaccaga ttcgctcccg cgctgtttcc catgaggacg atcttgttca
     3781 cgtctccgcc aaaccgctgc gcgttttccg atatccacaa aaagctcgcg tagctgtcat
     3841 cgaccgccgc gggaaatcga tgctccggcg cgacgcggta atccaccgca aacaccaccg
     3901 cctcgtagtt cctggcatcc tgccagagcg aatactcgag tccgggtacc agcggcgaaa
     3961 tgaaaccgcc tccatgatat tggatgatga ccggaagatt gtccttatgc agcgggttga
     4021 acacgaggac cggaatacta tcccgggtca ctttg
//
LOCUS       DAOTON010000148         4055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17826_length_4055_cov_2.868250, whole genome shotgun sequence.
ACCESSION   DAOTON010000148 DAOTON010000000
VERSION     DAOTON010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17826_length_4055_cov_2.868250"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="P5292_04085"
     CDS             complement(<1..190)
                     /locus_tag="P5292_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRS38337.1"
                     /translation="MLKCLLRKTPLLGLMVVLLEACNPTGNGSDGRQVFRFNDASGIL
                     TLDPAFAKDQSHIWASMLF"
     gene            346..1695
                     /gene="mtaB"
                     /locus_tag="P5292_04090"
     CDS             346..1695
                     /gene="mtaB"
                     /locus_tag="P5292_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRS38338.1"
                     /translation="MNRRPSVSFHTLGCKLNFAETSTIGKQLRDAGFQKTEFDSGADV
                     YVINTCSVTENADKECRTLVRRALQRNPNAFVAIIGCYAQLKPEEISRIPGVDLVLGA
                     TEKFNLPAYLNDLKKSGSTQVHSCEVSDATRFIASESAGDRTRAFVKVQDGCDYPCTY
                     CTIPLARGHSRSDTIDNVVARVKALAATGIREVVLTGVNLGDFGLRHLEGGGSERPEH
                     FLQLAEALEQVEAIDRFRISSIEPNLLRDEIIRFVAQSTRFVPHFHLPLQSGSDKILA
                     AMKRRYRTGLYTERVEAIRQLMPHAAIGVDVITGFPGEGEAEFMETFNFLHGLPVSYF
                     HVFTYSERDHTEAATLPGKVPMEIRKDRTRQLRNLGDKKYRSFIESFIGQTRPILWEA
                     EQVNGRMHGYTDNYIRVSTPFAAAAVNTVVPTELGEVDPNGTPCLMCRNVEALIHVS"
     gene            1739..2899
                     /locus_tag="P5292_04095"
     CDS             1739..2899
                     /locus_tag="P5292_04095"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRS38339.1"
                     /translation="MYPGISDLFNELFGTHFDFSFPPSFGTLVAISFALAAWTLGLEL
                     KRKEREGRLQPVKRTVVIGTPASFSELASNGLFGFILGAKALLIISDSSAFLQDPPGI
                     LLSLKGSWTGGIAGGLLFAYLKYREKKRVQLPAPKTIQETVYPHQQVTELTMMAALGG
                     LVGAKLFHILEYWHAFLQDPLDMIFSGSGLTMYGGLIVGAIAVLYYGRKQGIPPLELC
                     DATAPGLMLAYGTGRIGCQLAGDGDWGIVNHAPKPDWLSFIPDWAWSSTYPHNVLKEG
                     VPIPGCTGRHCFELPEPVFPTPLYESILCIGFFFLLWAFRKRLRLPGQVFGWYLILNG
                     LERFAIESIRVNSLYHVAGISFTQAQLISVLLFISGLTLLTLIRRDRPKQHA"
     gene            2892..3692
                     /locus_tag="P5292_04100"
     CDS             2892..3692
                     /locus_tag="P5292_04100"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRS38340.1"
                     /translation="MREDLQLVCSAVTSLTGKVGQFILQERKRFRADRVEKKGHNDLV
                     SYVDKEAERMLVDGLADILPDAGFLTEEKTRTDEGNGLRWIIDPLDGTTNFIHGIPCY
                     CISIGLFDGNKGLVGVVHELNLDECFYASADNGAYLNGEPIRTSNVPSLADSLIATGF
                     PYHDYDRMKPYMEVFDHCMRHTRGLRRLGSAAADLAYVAAGRFEIFYEYGLNPWDVAG
                     GIVLVREAGGIVTDFSGEDNPLYGAEIIAGGPEIQNSFLDVVRERFRP"
     gene            complement(3699..>4055)
                     /locus_tag="P5292_04105"
     CDS             complement(3699..>4055)
                     /locus_tag="P5292_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38341.1"
                     /translation="VESEVKPKTDARPVPEPDYAFTNGRKKVTEQGMAGWVGDEDINP
                     NRYYALHRSAPNGTIIRVTNRMNNANVFVKVVGRLPDSGENEGMIIKISKAAADKMGV
                     IDQRFQVDLLYGIQEK"
BASE COUNT          899 a         1073 c         1088 g          995 t
ORIGIN      
        1 agaacaacat ggatgcccaa atatgcgatt ggtctttcgc gaaggcaggg tcaagtgtca
       61 ggatcccgct ggcatcgttg aaacgaaaga cttgtcggcc atccgaacca ttaccggtcg
      121 ggttgcaggc ctccaggagc acgaccatga gtccgagcaa aggagtcttt cgaagtaagc
      181 acttcaacat agggtcctca agttatagca gttggcgtat tggatggttc cttttcatca
      241 gcagttttaa acgtatccgg gtggacgtga gattattctg aacgactgaa aaacaagcag
      301 ttaacagctt ttcgatgcat tggccgtttc gtacctttgc gacccatgaa tcggaggccg
      361 agcgtttcat ttcacaccct gggttgcaaa ttgaattttg ccgagaccag tacgatcggc
      421 aagcagctac gcgatgccgg ctttcagaaa accgaattcg attccggtgc ggatgtctat
      481 gtgatcaaca cctgttccgt tacggagaat gccgacaaag aatgtcggac tctcgttcgt
      541 cgtgctttac agcgaaaccc gaacgcgttt gtcgccatca tcggatgtta tgcgcagctg
      601 aagcccgagg agatatcccg catcccggga gtcgacctgg tgctgggtgc aaccgaaaag
      661 ttcaacttac ccgcatacct gaacgatctg aagaaatcag gttctactca ggtgcacagt
      721 tgcgaggtaa gcgacgcgac acgattcatc gcgtccgaat cggccggcga ccgtacccgc
      781 gctttcgtga aagttcagga tggctgcgac tacccctgca cctactgtac catccccctg
      841 gcacgtggac atagccgaag cgataccatc gacaacgtag ttgcccgtgt aaaggccctc
      901 gcggcaacgg gcattcggga agtggtcctg acgggtgtca atttgggtga tttcgggctg
      961 cgtcacctcg aaggaggcgg atcagagcga cccgagcatt tcctccaact ggcggaggct
     1021 ctggagcaag ttgaagcgat cgaccgcttc cgtatctcct cgatcgaacc caatcttttg
     1081 cgggacgaga tcatccggtt cgtcgcgcag tcgacccggt ttgttccaca tttccatctg
     1141 ccgttgcaat cgggatctga taagatcctg gcagcgatga aacgtcgtta ccggaccggc
     1201 ttgtatactg aacgagtcga agcgatccgg caattgatgc cgcatgcggc catcggcgtt
     1261 gatgtgatca ccggctttcc gggggaaggc gaagctgaat tcatggaaac gttcaatttc
     1321 ctgcatggct taccggtatc gtatttccat gtattcacct attcggagcg tgatcatacg
     1381 gaggcggcta ccctgccggg gaaagtgccg atggaaattc gaaaagatcg tacacggcag
     1441 ttgcgtaatc tgggcgataa aaaataccgt tcgttcatcg aatcgttcat cggacaaaca
     1501 cgcccgatcc tttgggaggc cgagcaggtg aatggaagga tgcatggcta taccgataat
     1561 tacatccggg tcagcactcc ttttgcagcg gctgcggtaa atacggttgt tcccaccgaa
     1621 cttggagaag ttgatcccaa tggtacaccc tgcctgatgt gccggaatgt agaagcattg
     1681 atccatgttt cctgacctcc tcacccaccg tcgctgacga tcagcacccc tacttcacat
     1741 gtatccaggc atcagcgacc tcttcaacga actgttcggc acccatttcg atttcagttt
     1801 ccccccttcg ttcggcacgc tcgtcgctat cagctttgcg ctggctgcct ggacacttgg
     1861 tctggaacta aaacgaaaag aacgtgaagg ccgcttgcaa cctgtgaaac ggacagtggt
     1921 gatcggaacc cccgcctcct tttcggaact ggcctccaac gggctcttcg gtttcatttt
     1981 gggtgccaaa gccttgctga tcatttcaga cagcagcgcc tttttgcagg accctcccgg
     2041 gatcctgctt tctttaaaag ggagttggac tggaggtatt gccggaggat tgttgttcgc
     2101 ttatctgaag taccgggaaa aaaagcgagt ccagttaccc gctccaaaaa ccattcaaga
     2161 aacggtatat ccacatcagc aggtcactga attgaccatg atggcggcgc ttggcggact
     2221 ggttggcgcc aagctcttcc atatacttga gtactggcat gccttcctac aggatccgct
     2281 tgatatgatc ttcagcggca gcggacttac gatgtatgga ggtctgatcg tcggtgcgat
     2341 agcggtgttg tattatggcc gaaaacaagg cattccgccc ctggaattat gcgacgcgac
     2401 cgctcccggt ctgatgctgg catatggtac cggccgtatc ggttgtcaac tggccggcga
     2461 tggggactgg ggcatcgtca atcatgcacc aaagcccgat tggctgagtt tcattccgga
     2521 ttgggcctgg tcctccacct atccacacaa tgttctcaag gaaggtgtac ccatccccgg
     2581 atgtaccggc aggcattgtt tcgaattacc ggaaccggta tttcctacgc cactctatga
     2641 aagcattttg tgcattgggt tcttcttttt gctttgggca ttccgaaaac gactgcgctt
     2701 accgggacag gtgtttggat ggtacctcat cctgaatggc ctggagcgct tcgccatcga
     2761 gtccattcgt gtcaattccc tctaccatgt tgccggtatt tcctttacgc aggcgcagct
     2821 tatttctgta ttgcttttca tttccggact gaccttgctt acccttatcc ggcgagaccg
     2881 cccgaagcaa catgcgtgag gacctccaac tggtatgcag tgcggttacc agtctgaccg
     2941 gaaaggtcgg tcagttcatt ctacaggaac gtaagcgctt tcgcgcggac cgtgtggaaa
     3001 agaaaggaca taacgacctg gtttcgtatg tggacaaaga agctgagcgt atgctggttg
     3061 acggattggc ggacatcctg ccggatgccg gattcctgac ggaggaaaag acacgtaccg
     3121 atgaaggaaa tggcttacgc tggatcatcg acccacttga cggcactacc aatttcatcc
     3181 acggtatccc ctgctattgt atcagcatcg gcttgttcga cggaaacaaa ggccttgtag
     3241 gagtcgttca tgaattgaac ctggacgagt gcttctatgc ttcagccgac aatggcgcct
     3301 atctgaatgg agaaccgatc cggacttcga acgtcccgtc attggcggat tcgctgatcg
     3361 ctacaggatt cccttaccac gattatgacc gaatgaagcc ctacatggaa gtcttcgatc
     3421 attgtatgcg tcatacgcgg ggtttacgca gactggggtc tgcagcggct gatcttgcct
     3481 atgtggcagc gggccggttc gaaatttttt acgaatatgg attgaacccg tgggacgtcg
     3541 caggcgggat cgtgctggta cgggaggctg gcgggatcgt aacggatttt tcgggcgagg
     3601 ataacccgtt gtacggtgcc gagatcattg ccggcggacc ggagatacaa aacagcttcc
     3661 tggatgtggt acgggaacgg tttcgtccct gaaagaactc acttctcctg aattccgtag
     3721 aggaggtcaa cctggaatcg ctggtcgatc acgcccatct tgtcggccgc ggctttggaa
     3781 atcttgatga tcatgccttc gttctcaccg gaatccggca atcgtccgac cactttcaca
     3841 aacacatttg cattgttcat ccggttggtg acccggataa tggtaccatt cggtgcactg
     3901 cggtgcaaag cgtaataacg gttgggattg atatcctcgt cgccaaccca tcccgccatg
     3961 ccctgttcgg tcactttctt tcgtccattc gtgaaagcgt aatccggctc cgggacgggt
     4021 cttgcatcgg tcttcggctt tacctcggac tccac
//
LOCUS       DAOTON010000149         4051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17852_length_4051_cov_3.452452, whole genome shotgun sequence.
ACCESSION   DAOTON010000149 DAOTON010000000
VERSION     DAOTON010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17852_length_4051_cov_3.452452"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..940
                     /locus_tag="P5292_04110"
     CDS             191..940
                     /locus_tag="P5292_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HRS38342.1"
                     /translation="MEQRILELLKRARRENRKLFAVLVDPDKSDDRSLDVLTKTGKEA
                     GIDLFLVGSSILRDDRFDSTLLRLKQQAHCPVIIFPGNAMQVSEHADGILLLSLISGR
                     NADLLIGQHVLAAPFLRNSGIEIIPTAYLLIDSGAPTSVSYMSQTLPIPHDKDDIASC
                     TALAGEQLGLQVLYLDAGSGARHPVSARMIRNVRQVTNIPLVIGGGIRTPEKAAELAQ
                     AGADMIVVGNSLETDPGMLQELADAVHLTVR"
     gene            complement(1117..2049)
                     /locus_tag="P5292_04115"
     CDS             complement(1117..2049)
                     /locus_tag="P5292_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRS38343.1"
                     /translation="MSLLNKFIDSLQIRFLGSAFREVIHPFAFQGRMEQHNVIYMLNK
                     GHLSVGPHAVPVSPGDFYFFPAGQQHQLRFGSGRQTVIGPEGFAGAHPREEFLRDVSC
                     SEDFSQLKELYTEISFEVLLYNAIPFFEILQLPAIPMPADLEFGHLIRYITQEQEQNK
                     LGRDKIISNYMEEIIIHLCRYIDSQARFRPYVERLEFLADRRLVDIVKYIQGNLEKDL
                     SNKVIANIAYVSEDYVGQFFKSLTGQNLQDYIENQRLERAMQLLRTQPDNIQEIAHRV
                     GFKDPAYFSRRFKLKFNANANSIRQFARKDAIGE"
     gene            2289..2885
                     /gene="pnuC"
                     /locus_tag="P5292_04120"
     CDS             2289..2885
                     /gene="pnuC"
                     /locus_tag="P5292_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRS38344.1"
                     /translation="MQEIVSMFNQQMTWLEFAGMLTGLAGVWLTVRASVWCFPTGILC
                     VSIYAYIFFHPTVRLYADGILQLIFLPLQTIGWINWRTRRETTTRITRTPRKEWGILF
                     LVFLAGWFFLSSMLHRYTNAYLPWLDGALTTLSLIAQWQIARKQLENWLLWIFVDILY
                     IPLYFSRDLPLTAFLYLIFLFMAIAGYRAWRKQLASDA"
     gene            2878..3414
                     /locus_tag="P5292_04125"
     CDS             2878..3414
                     /locus_tag="P5292_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS38345.1"
                     /translation="MPEHPIVRIALLGPESTGKSTLAERLAEQYQTVWVPEFARTYLA
                     TLDHPYTAADVLHCFREQVAAEDSATAAASRLLFCDTEIINFRVWLLYRFGHCPSFVS
                     DAWTHRYDFYLLTYPDIAFEPDPLREHPGRRLFFFDWYRRELEMRGANYSVIKGEGEA
                     RIRNAKLALDQFLAARAQ"
     gene            complement(3408..3935)
                     /locus_tag="P5292_04130"
     CDS             complement(3408..3935)
                     /locus_tag="P5292_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38346.1"
                     /translation="MKKYLIVFLFGCLSAQFASAQLYYAAIGGRAGKFNTGVTFKYFA
                     NTNNAIGYQIDAYYTNIASGGYTAKGFVIKQIPFKLPIVQLPLDFIVGGGAHAGYFPF
                     DPQGYYKRNGDEADYYEKDVVSVGVDATIQIEYQVRKVVPLTIGIDATPFFEFLNPGP
                     EWIDFGVSLRYVFRY"
BASE COUNT          952 a         1115 c         1033 g          951 t
ORIGIN      
        1 tggattttcg aaaagagctc gaagtatcca gcttccagat cggagaatcg ggaacactac
       61 gggcagcgat tcgaaagaca gacttcaatc tggacacgtt gatccactat cgctttttcg
      121 acgacatgct cctggtttat accgcccatc cggcatgaca gtaagcgttt tcgtacttta
      181 gccagagcgt atggaacaac ggatcctcga attgctcaaa cgtgcccggc gggaaaaccg
      241 gaagttgttc gccgtcctgg tggatcccga taagtcggac gaccgaagcc tcgatgtact
      301 gacgaaaacc ggcaaggaag ccggtatcga tctcttcctg gtcggcagca gtatcctgcg
      361 cgacgaccgc ttcgattcca ctctcctccg actgaaacag caggcgcatt gcccggtgat
      421 catctttcca ggcaacgcga tgcaggtaag cgaacatgcc gacgggatct tactgctcag
      481 cctgatcagc ggtcgtaatg ccgacctgct catcggacaa catgtcctcg ctgcgccctt
      541 cctgcgaaac agcggaatag aaatcatccc caccgcttac ctgctcatcg acagcggcgc
      601 tccgacctcg gtcagctata tgagtcagac gctgcccatc ccgcacgaca aagacgatat
      661 cgcctcctgt acagcattgg ccggcgaaca actcggtttg caggtattgt acctggatgc
      721 cggcagcggc gcccgacacc cggtcagcgc ccgcatgatc cgcaacgttc gccaagtgac
      781 gaacataccg ctggtcattg gtggcggtat ccgtacaccc gaaaaagcgg ccgaactcgc
      841 gcaagccgga gctgatatga tcgtcgttgg aaactccctc gaaaccgatc ccgggatgtt
      901 gcaggaactc gcggatgcgg tacacctgac ggtgcgatag aagtttcggg ttccgggttc
      961 cgggtttcgg gtttcgagtt ccgatttaaa agagtacgat gaatgaaatg ttatcctgag
     1021 cagatagagc tttcagaatt tagggaattg acagaaataa ccggaaactg gaaactggaa
     1081 accggaaacc ggaaacgcgg aactatttcc cctcccttac tcgcctatcg catctttccg
     1141 tgcaaactgc cggatcgagt tggcgttggc gttgaatttt agtttgaagc ggcgactgaa
     1201 gtaggcagga tctttgaatc ctacgcggtg ggcgatctcc tggatgttgt cgggttgtgt
     1261 tcgcaggagt tgcatcgctc gctcgaggcg ctggttctcg atatagtcct ggagattctg
     1321 gcccgtcagg gatttgaaaa actgtccgac gtagtcctcg ctgacatacg cgatattcgc
     1381 gatcaccttg ttggataggt ccttttccag gttgccctga atgtatttta cgatgtcgac
     1441 cagacggcgg tcggcgagaa actccagccg ctccacatac ggacgaaacc tcgcctgtga
     1501 gtcgatgtac cggcacaggt gaatgatgat ctcctccatg tagttggaga taatcttatc
     1561 ccgtcccagt ttgttctgct cctgctcctg tgtgatgtag cggatcagat ggccgaattc
     1621 cagatcggcc ggcatgggaa tggccggcag ttgcaggatc tcgaagaacg gaatggcatt
     1681 gtagaggagg acttcaaaag atatctccgt gtacaactct tttagctggg aaaagtcttc
     1741 cgagcatgat acatcccgaa gaaattcttc acgcggatgc gcacctgcga atccttcagg
     1801 accgatgacg gtctgtcgtc ccgaaccgaa cctcaactga tgttgttgtc cggcagggaa
     1861 gaaatagaaa tcacccggcg aaacgggaac agcatgcgga cccaccgaca ggtggccctt
     1921 gttcaacatg tagatcacat tgtgctgttc catcctaccc tggaaggcga acggatgaat
     1981 cacttcgcga aaggcagatc cgagaaaacg gatctgaaga gagtcaataa acttgtttag
     2041 cagcgacata atacccccca aatttgaaag cggttgctcg ctacaaacaa cctcccccga
     2101 attagcagta ccgaaagtta gccatttcat cttggtatta tccaagtctg gtacggataa
     2161 attcaattga taaggcgcaa gattttcctg aattcaagcc gaatttcaat tttgacaccc
     2221 tcctgacaaa atcagggctc cataaccgtc aaaattcgcc cttcactcga attactcatc
     2281 tttgcagggt gcaggaaata gtcagcatgt tcaaccagca aatgacctgg ctggaatttg
     2341 caggaatgtt aaccggtctg gccggagtat ggctcaccgt tcgcgcatcg gtgtggtgct
     2401 ttccgacagg catcctgtgc gtttcgatct acgcgtatat tttttttcac cccactgtcc
     2461 ggttatacgc cgacggtatc ctgcagttga tcttcctacc gttgcaaacc atcggctgga
     2521 taaactggag gacaaggagg gaaacgacca cccggatcac cagaacgccg cgcaaggaat
     2581 ggggcatact cttcctggtt tttctcgcgg gctggttctt tctctcttcc atgctgcatc
     2641 gttacaccaa tgcttatcta ccctggctgg acggtgcgct gacaacactt tcactgatcg
     2701 cccagtggca gatcgcacga aaacaactgg agaactggct cctttggatc ttcgtcgaca
     2761 tcctgtatat tcccttgtat ttctcccgtg acttgcccct gaccgccttc ttatacctga
     2821 tcttcctgtt catggctatc gccggatatc gcgcctggag aaaacaactc gcttccgatg
     2881 cctgaacatc cgatcgtacg catagccttg ctcggtcccg aatcgacggg gaagtccacc
     2941 ttggccgaac ggctggcgga acaatatcaa acggtctggg taccggagtt cgcgcgcacc
     3001 tacctcgcaa cactcgatca tccctacaca gcagcggatg tgttgcattg tttccgggaa
     3061 caagtcgcgg cagaagatag cgctacggct gctgccagcc ggttgttgtt ttgcgatacg
     3121 gagatcatca acttccgtgt ctggttgctc taccgcttcg gccattgccc ttcgttcgta
     3181 tccgacgcct ggacccatcg ttacgatttc tacctcctca cctatcccga tatcgctttc
     3241 gaaccggatc ccttacggga acatccgggg cgtcgcctct tcttctttga ctggtaccgg
     3301 cgtgaactgg aaatgcgcgg agcgaactac tccgtgataa aaggcgaagg tgaagcgcga
     3361 atccgaaacg cgaaactcgc gctggatcaa tttcttgctg cgcgggctca gtagcggaaa
     3421 acgtatcgaa gggaaacccc gaagtcgatc cattccggac cggggttcag gaactcgaaa
     3481 aaaggcgtcg cgtcgatacc gatggtcagc ggcactacct tgcgcacctg gtattcgatc
     3541 tggatcgtag cgtcgacacc cacggataca acatccttct cgtagtaatc ggcctcatcg
     3601 ccgtttcgct tgtaatatcc ctgcggatca aaggggaaat agccggcatg cgctcctccg
     3661 ccgacaatga aatcgagcgg caactgaacg atcggcaatt tgaacgggat ctgcttgatc
     3721 acaaaaccct ttgccgtata accgccggag gcgatgttgg tatagtacgc gtcgatctgg
     3781 tagccgatgg cattgttcgt attcgcgaag tatttaaagg tgacccccgt attgaattta
     3841 ccggcccgtc caccaatggc cgcataatac aactgcgcac tggcgaactg ggcgctcaaa
     3901 caaccgaaga ggaatacgat cagatatttt ttcatgccaa gacgaaaata gcgaattagt
     3961 ccgtatccgg tgctatcgta ttcttgctac ctttgtcctc atcactgggg tgccccctga
     4021 tccacggggg ctgagatcat acccatcgaa c
//
LOCUS       DAOTON010000150         4043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17895_length_4043_cov_3.520562, whole genome shotgun sequence.
ACCESSION   DAOTON010000150 DAOTON010000000
VERSION     DAOTON010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17895_length_4043_cov_3.520562"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="P5292_04135"
     CDS             complement(<1..909)
                     /locus_tag="P5292_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38347.1"
                     /translation="MQPRRIRRPLLSTVLLIISLIANFSAFADEPGLVVLDVTLIRNR
                     HTRDHILLRELTFAKGDTLPTGELEKIFKRSEDNLFNTSLFNSTKITWLRDGDGIRVF
                     VIVTERWYIFPVPIIEVAERNLNTWWKTKDYSRLIYGGSLAWNNFRGRRETFSTTLRL
                     GYTQRMNFYYSVPFLDKARQWGASVSAAFSRNRQNVYNTVDNKQLNFRWDDVYSKNEY
                     SFSGAVSYRPDLYESHFVEAGFRHIEIDDTLLKLNPDYLINGQHTQRAISLRYLFRVE
                     HRDLVVYPLKGTYFDIELGKNGFPVLG"
     gene            complement(919..1581)
                     /locus_tag="P5292_04140"
     CDS             complement(919..1581)
                     /locus_tag="P5292_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRS38348.1"
                     /translation="MIARELVNDSIPPLKLSDTGLKALNWMEEFRVEHLAIVDGVSYI
                     GLISEEDILKLSALDQPLANSKLSLIRPYVRYNQPVFEVVRLMSKDKLTLVPVLDASD
                     HYLGLITLGDVLKHYSDSGIFEDANGVIVLEMSPKDYLLSAIAHLVESENARILSAYV
                     TPNPEHETIDVTIKINQPELTRILSSFTRHGYLVKEHYHQSEFMDDLKSRYDSLMNYL
                     GI"
     gene            1767..2498
                     /locus_tag="P5292_04145"
     CDS             1767..2498
                     /locus_tag="P5292_04145"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRS38349.1"
                     /translation="MTRLSVNINKIATLRNARGGNIPDVVKAAIDIQRFGADGITVHP
                     RPDERHIRRSDVYALREVVTTEFNIEGYPSADFLKLIREVHPEQATLVPDPPDVLTSN
                     AGWDTVRHAAQLKELIAEIKSYGIRTSIFIAPDPAMIEAAAAVGTERIELYTEAYASN
                     FSHDRAAAIAPYAAAAAKAREVGLGINAGHDLSLENLRYFTAGIHGLEEVSIGHALIS
                     DALYFGLENTVQLYLRALRGNVDPA"
     gene            2495..3262
                     /locus_tag="P5292_04150"
     CDS             2495..3262
                     /locus_tag="P5292_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRS38350.1"
                     /translation="MKLFFNRIGEGRPLIILHGLFGLGDNWATIARQLATHGFACYLV
                     DQRNHGRSPHSPDFSYPLMSEDLFELLQDQRISQPILLGHSMGGKTVIRFALDHAGIA
                     IKLVVVDIAPRYYPPHHQAVLAALHAVDFDQVRKRKEVEDMLRNALHEEATVQFLLKN
                     LYWVSDDRLAWRFDLDTLSRSIDSVGDAMPNDRVIETPTLFVRGERSGYITAGDEVEI
                     SRHFSTASVVTVPAAGHWVHAENPKGFLEAVLPFLNS"
     gene            complement(3326..>4043)
                     /locus_tag="P5292_04155"
     CDS             complement(3326..>4043)
                     /locus_tag="P5292_04155"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HRS38351.1"
                     /translation="GNGPMTAIFTIYSGDPELILRTTEDVSGMTDTRCDGTSGTLTVM
                     SIDSVRLAYTGTTTTAPSGKKIRGIVTSDYTNSNLVSQNLTIQDATGAIVVRFGSSHS
                     FTLGTEIEVNISGQELSEFNGWLQVNNVPLGNATAVGTGTVTPRTATIAQVLANYDAW
                     ESQVIKITGVTISGTGTYSGSQTLSDGSGTITMFTRTGASFSSQSYPTGTVSVTGILS
                     EYSPSTFTPQIIIRNTSDVQ"
BASE COUNT          872 a         1054 c         1189 g          928 t
ORIGIN      
        1 gccgagtacc ggaaacccgt tcttaccgag ctcgatatcg aaataggtcc ctttgagggg
       61 atataccacc aggtcgcggt gctccacccg aaaaaggtaa cgaagcgaaa tggcgcgttg
      121 cgtatgctgc ccgttgatca ggtaatcggg attgagtttc agcagcgtat cgtctatttc
      181 gatgtgacga aatccggctt ccacaaaatg tgattcatac agatcgggtc ggtaagagac
      241 ggctcccgaa aaactgtatt cgtttttgga atagacgtcg tcccagcgaa aattaagctg
      301 cttgttatcg acggtattgt aaacgttctg tcggtttctt gaaaacgcag cgctcaccga
      361 ggccccccac tgccgggcct tgtcgaggaa aggaacgctg taatagaaat tcatgcgttg
      421 tgtatagccc aggcgcaggg tggtggagaa ggtctccctc cgtccccgga aattgttcca
      481 ggccagggat ccgccataga taagccggga gtaatccttg gttttccacc aggtgttgag
      541 gttgcgttcc gccacttcga tgatggggac aggaaaaatg taccagcgtt cggtgacgat
      601 gacaaacacc cggataccgt cgccatcgcg cagccaggtg atcttggtgg agttgaagag
      661 cgaggtgttg aatagattgt cctccgaacg cttgaagatc ttctccagtt cgccggtcgg
      721 gagcgtatcg cctttggcaa aggtgagttc ccgcaggagg atgtggtcgc gtgtgtgccg
      781 gttcctgatc agcgtgacat ccagcaccac gagtccgggc tcatcggcaa aggccgagaa
      841 attcgcgatc agggaaatga tcagtaaaac cgtgctgagt aacggcctgc ggatgcgccg
      901 gggctgcatt ccgtaaagtt aaatgcccag gtaattcatg agcgaatcat agcgtgattt
      961 caaatcgtcc atgaattcgc tctgatggta atgctccttg acgaggtatc cgtgacgggt
     1021 aaaggaagaa aggatccggg tgagctcggg ctggttgatc ttgatcgtca cgtcgatcgt
     1081 ctcgtgttcc ggattgggcg tgacgtaggc ggagaggatc cgcgcgttct ccgattccac
     1141 cagatgggca atggccgaaa ggagataatc cttcggactc atctcgagaa cgatcacgcc
     1201 gttggcgtct tcgaagatgc cgctgtccga gtagtgtttc aggacatcgc ccagggtgat
     1261 gaggccgaga taatgatcgg atgcatcaag caccgggacg agcgtgagct tgtctttcga
     1321 catcagccgc actacttcga agaccggctg gttgtaacgt acgtaagggc ggatgagcga
     1381 gagtttggag ttggccaacg gctggtccag cgcgctcagt ttcaaaatgt cttcctcaga
     1441 gatcagcccg atataactga cgccatcgac gatcgccaga tgttcgacac ggaactcctc
     1501 catccagttc agcgccttca gtccggtatc gctgagcttg agtggaggga tcgagtcgtt
     1561 gacgagttcg cgcgcgatca tgttgggttt acgttgtaag tacccggaag gttttcgggc
     1621 gacaagtata cgcatttttc gtgccgatgc cgggaacggc gcgcccgatg cgagttttat
     1681 gacattctgc ccctttcatc agtaaaacac gcagtcgttt gcattgttgt aacagaaccg
     1741 ggaaatttgg tcttctttac aaacggatga cccggctcag cgtcaacatc aacaagatcg
     1801 ccaccctgcg gaacgcgcgg ggcggaaaca ttcccgacgt ggtgaaggcc gcgatcgaca
     1861 tccagcgttt cggcgcagac ggcattactg ttcatcctcg acccgacgag cgacacattc
     1921 gtcgatccga cgtatatgcg ttgcgtgaag tggttaccac cgagttcaac atcgagggat
     1981 atccatccgc cgacttcctc aagttgatcc gtgaggtcca tcccgagcag gctacgctgg
     2041 tacccgaccc gcccgatgtc ctgacatcga acgccggttg ggacacggtt cgccatgctg
     2101 ctcaattgaa agagttgatc gccgagatca aaagttacgg catacgcact tcaatcttca
     2161 tcgcgccgga tccggcaatg atcgaagcgg ccgctgccgt tggcacggaa cgcatcgagt
     2221 tatacacgga ggcttatgct tccaacttct cacacgatcg tgcggcggcg atcgctccct
     2281 acgcggcggc ggcggcgaaa gcccgggagg taggcctcgg catcaacgcc ggacatgatc
     2341 tcagtctgga aaacctgcga tatttcacag caggcattca cggactggaa gaggtttcca
     2401 tcgggcatgc gctgatcagc gacgcgctct attttggatt ggagaataca gttcaacttt
     2461 atttgcgtgc cttgcgcggc aacgtggacc ctgcatgaag cttttcttca ataggatcgg
     2521 cgaaggccgg ccgctgatca ttctgcatgg cttgttcggc ctgggggata actgggcgac
     2581 catcgctcgt caattggcta cccatggatt tgcctgttac ctggtcgacc agcgaaatca
     2641 tggccgcagt ccgcacagtc cggatttcag ttatccgttg atgtcagaag accttttcga
     2701 gttgctgcaa gaccaacgaa tcagccaacc gatcctgctc ggacattcca tgggcggcaa
     2761 gacggtcatc cgatttgcat tggatcatgc ggggattgcg atcaaactcg tagtggtcga
     2821 tatcgctccg cgttactatc ctcctcacca ccaggcggtg ttggccgcct tgcacgcggt
     2881 ggattttgac caggtgcgaa agcgcaagga agttgaagac atgctgcgaa acgcattgca
     2941 cgaagaagct actgtgcagt tcctgctcaa gaatctctac tgggtcagcg acgaccggct
     3001 cgcctggcga ttcgatttgg atactctttc ccgctcgatc gactcggttg gcgatgccat
     3061 gccaaacgac cgggtgatcg aaaccccgac cttatttgtg cggggcgagc ggtccggtta
     3121 tatcacagcg ggcgatgaag tggagatcag ccgacatttc tccacggcga gcgtggtcac
     3181 cgttccagcc gccggtcatt gggtccatgc cgagaatccc aaaggctttc tggaagccgt
     3241 gctccccttt ctgaattcct gatcgatgag aataaaaaaa gccatacgcg gcttgcgtat
     3301 ggcttttctc attcagcgaa gtggcttact gtacgtcgct ggtattgcgg atgatgatct
     3361 gtggtgtgaa cgtgctgggt gagtactcgg ataggattcc ggtaaccgaa accgttccgg
     3421 tcggatacga ctgagacgag aacgatgcgc cggtacgcgt aaacatggta attgtgccgg
     3481 agccgtcgct gagtgtctgc gatccactgt aggtgccggt tccactgatg gtaactccgg
     3541 tgatcttgat tacttgtgat tcccaggcat cgtaattggc caggacctgc gcgatcgtgg
     3601 cggtgcgagg ggtcacggtg ccggtgccga cagccgtggc gttgccgagc ggaacgttgt
     3661 tcacctgcaa ccaaccgttg aactcagaca attcctgtcc cgagatgttg acctcgatct
     3721 cggtgcccag cgtaaagctg tgactggaac cgaaacgaac aacgatagcg cccgtagcgt
     3781 cctgaatggt aaggttctga cttaccaggt tgctgttggt gtagtcgctg gtaacgatgc
     3841 cccggatctt cttaccggaa ggcgccgtgg tggtggtgcc cgtgtaggca agtcgaacac
     3901 tgtcgatgct catgacggtc aaggtgcccg acgtaccgtc gcagcgtgtg tcggtcatgc
     3961 cggagacgtc ttcggtggtg cgtaggatca gttccgggtc gccgctgtag atggtgaaga
     4021 tggccgtcat cgggccgttg ccg
//
LOCUS       DAOTON010000151         4041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17909_length_4041_cov_3.482689, whole genome shotgun sequence.
ACCESSION   DAOTON010000151 DAOTON010000000
VERSION     DAOTON010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17909_length_4041_cov_3.482689"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..3637
                     /locus_tag="P5292_04160"
     CDS             287..3637
                     /locus_tag="P5292_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 domain-containing protein"
                     /protein_id="HRS38352.1"
                     /translation="MAPDSYRLLIEKLDGFIRKYYQNQLIRGAIYAFTLSLGFFLLVT
                     LLESFGHFSIGLRTFLFWAFILGIGFLLVRFVAIPLAHLYRIGKIISHEEAARIIGKH
                     FSHVQDKLLNVLQLRELAGSAGDNALIEASISQKVDELRPVPFVAAVNLRENRRYLRY
                     AALPLAVLLVLLFAAPSLITDSTKRLIEHRTYFEKPAPFQFVVENKSLRAIQQEDFRL
                     DVRIEGKEIPAEASIELDGKLFRLEKESATKFHYNFRNLQQPQEFRLTADGFYSSTYQ
                     LEAVPKPTLMNFQVRLDYPAYLNKKSETLENNGDLVLPAGTRVTWTFNTRNTDVFRLA
                     FGDTLLALQPEGEERFSLSRRFLHNDSYALLPSNRFLGSPDTVKYGITIVPDQYPTIT
                     MEEQQDSLSPKRTFFRGLVRDDYGFSALSFHYHFLKSNDTTGANRNRTFSENLPVNRS
                     LTTDAFYYSWDLSSVNIQAGDEVEYYFEVRDNDGVSGPKAARTPVRVFKAPSLREIAE
                     SSEKSNQSIKSDLTESIRKAQQLQKDVNDLNRKLLEKKEASWEDRKKAEELLKREQEL
                     RKQLEEISKQNQEKNRQEQEYKRVNEDILAKQEQLQSLLDKLMSPELEKLMQQLQELM
                     QQLDKTQLQEQLGQMKNDNKDLQKELERTLELFKQMEFEQKMQESIDKLKDLAKKQDD
                     LSKQADDKNADSKDLQAKQDELNKEFDDFRKDMDELAKKNDALEEKQNLENTDQQEQE
                     IQQEMENSSQQLNQGKNNKAGKSQKNAAQKMDQLAQKMEKNQQQMEQEQAEEDMDALR
                     ALLENLLRMSFDQEQLMTDLKGMDINNPQFLRAGQEQRRLKDNARVIEDSLFALSKRV
                     MQIQAKVNQEIAAINLNMGEAIDNLEERNVPQARSRQQFVMTSVNNLTLLLSEALQQM
                     QQQQANAQPSASCKKPGKKKSSSIAEMKKMQQELNKNMQKMKDQLKQQGSKSQGKISK
                     DQSMSEQLAKMAAQQEYIRQQLQQLNQEDNKDGRKSLGNLEDIANQMEQTEKDIVNRM
                     ISDQTLRRQEEILTRLLESERAERERDQDEQRKSEEAKNRFERNPAAFEEYKRLKLKE
                     MELLRTVPPSLSSFYRQKVSDYFQSIEK"
     gene            3627..>4041
                     /locus_tag="P5292_04165"
     CDS             3627..>4041
                     /locus_tag="P5292_04165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS38353.1"
                     /translation="MKNSELLMQGNDQLQTLELRSQPDSIGAVERMIDQLRDQYEVSE
                     DMYGNMLVALTEAVTNAIYHGNQSDPGKKVHIKYGRVNNSLNFTISDEGRGFDYYNLP
                     DPTAPENLEKECGRGIFLMKHLTDQLIFSENGRVVE"
BASE COUNT         1073 a         1253 c         1046 g          669 t
ORIGIN      
        1 ctactgccta ccacctgccg gggactgtgt gacgaggatt cgaatccaaa ttcggtcacc
       61 tacctctcaa aacccccaat accccaacta ttcgctattc gctattcgct gttcgctccc
      121 gcctacgctc ccacctacgc taaagctccg gcgggcagga ttcactattc actgttcact
      181 gtttactgtt cactgttcac tacttccctt caacttttaa cgttccctta cgttactacc
      241 catcccccgc cctgctggcg aattcgtaga tttgtgatac tctattatgg caccggattc
      301 ctaccgattg ttgattgaga agctggacgg attcatccgg aaatactacc agaaccagct
      361 catccgtggc gcgatttacg cgttcaccct ttcgttgggc tttttcctgc tggtcaccct
      421 gctcgaatcg ttcggtcact tcagcatcgg gttgcgcacc ttcctgttct gggcgttcat
      481 ccttggcatc gggttcctgc tggtccgctt cgtcgccatc cccctcgccc atctgtaccg
      541 catcggcaag atcatctccc acgaagaagc tgcgcgcatc atcgggaagc atttttccca
      601 cgtgcaggac aagctcctca acgtcctcca gcttcgtgag ctggccggca gcgccggcga
      661 caacgccctg atcgaagcct ccatcagcca gaaggtcgac gagctgcggc cggtcccgtt
      721 cgtcgccgca gtcaacctgc gtgagaaccg ccgatacctc cgctacgcgg cgctgcccct
      781 ggcggtgcta ctcgtgttgc tcttcgcggc gccctcgttg atcaccgaca gcaccaaacg
      841 cctcatcgag caccggacct atttcgaaaa gccggcgcct tttcaattcg ttgtcgagaa
      901 caaatcgctc cgcgcgatcc agcaggagga cttccgcctc gacgtacgta ttgaaggaaa
      961 agagatcccc gccgaagcat ccatcgaact cgacggcaaa ttgtttcgcc tcgaaaaaga
     1021 atcggcaacg aagttccact acaacttccg caacctgcaa caaccgcagg agttccgcct
     1081 cacggccgac ggattctatt cgtccaccta ccagcttgaa gccgttccta aacccacgct
     1141 catgaacttc caggtgcgcc tcgactaccc ggcctacctg aacaagaaga gcgaaacgct
     1201 ggagaacaac ggcgacctcg tactgccggc gggtacacgg gtcacctgga cgttcaacac
     1261 ccgcaacacc gacgttttcc gtctcgcgtt cggcgacacg ctgctggcac ttcaacccga
     1321 aggcgaagag cggttcagcc tgagccgtcg cttcctgcac aacgacagct acgcgctgct
     1381 tccatccaat cgtttcctcg gctcaccgga tacggtgaag tacggcatca ccatcgtgcc
     1441 cgaccagtac ccgacgatca ccatggaaga acagcaggac tcgctttcgc ccaagcgcac
     1501 cttcttccgc ggactcgtgc gcgacgacta cggtttcagc gccctttcgt tccactatca
     1561 tttcctgaag tcgaacgaca ccacgggcgc caaccgcaac cgcaccttct ccgagaacct
     1621 gccggtgaac cgcagcctga ccaccgacgc gttctattat tcctgggatc tttcctcggt
     1681 caacatccag gccggcgacg aagtcgaata ttatttcgaa gtgcgcgaca acgacggcgt
     1741 gagcggaccc aaagccgcgc gtacaccggt gcgcgtgttc aaagccccga gtctgcgcga
     1801 gatcgccgag agcagtgaaa agagcaacca gtcgatcaag agcgacctca ccgaaagcat
     1861 ccgcaaagcg cagcaactgc agaaggatgt gaacgacctc aaccgcaaac tgctcgagaa
     1921 gaaagaagca tcctgggaag accggaagaa agcggaagaa ctgctgaaac gcgaacagga
     1981 actgcgcaag caactcgagg agatcagcaa gcagaaccag gagaagaacc ggcaggaaca
     2041 ggaatacaag cgcgtgaacg aggacatcct cgccaagcag gagcaactgc agtcgctgct
     2101 cgacaaactc atgtcgcccg aactggagaa actcatgcag caactccagg agctcatgca
     2161 acagctcgac aagacccagc tccaggagca actcggtcaa atgaagaacg acaacaagga
     2221 cctccagaaa gaactggaac gcaccctgga gttgttcaag caaatggaat tcgagcagaa
     2281 gatgcaggaa agcatcgaca agctgaaaga cctcgcgaag aagcaggacg acctttccaa
     2341 acaagccgac gacaagaacg ccgacagcaa ggatctgcaa gcgaagcagg acgagctgaa
     2401 caaggagttc gatgatttcc gcaaggacat ggacgagctg gcgaagaaga acgacgcgct
     2461 cgaggaaaag cagaacctgg aaaacaccga ccagcaggag caggaaatcc agcaggagat
     2521 ggagaacagc agccagcaac tgaaccaggg caagaacaac aaggcgggca agtcgcagaa
     2581 gaacgcagcc cagaaaatgg accagctggc gcagaagatg gaaaagaacc agcagcagat
     2641 ggagcaggaa caggccgagg aggacatgga cgccctgcgc gccctgctcg agaacctgct
     2701 gcgcatgtcg ttcgaccagg aacaactgat gaccgacttg aaggggatgg acatcaacaa
     2761 cccgcagttc ctccgcgccg gtcaggagca acgccggttg aaagacaacg cccgcgtgat
     2821 cgaagattcg ctcttcgccc tctccaaacg cgtcatgcag atccaggcca aggtgaacca
     2881 ggagatcgcc gcgatcaacc tcaacatggg cgaagcgatc gacaacctgg aagaacgcaa
     2941 cgtgccgcag gcgcgaagcc gtcagcagtt cgtgatgacc tcggtgaaca acctcaccct
     3001 gctactcagc gaagcgctcc agcagatgca gcagcagcag gcgaacgccc agccgtcggc
     3061 ctcctgtaag aagcccggca agaagaaatc gtcttcgatc gccgagatga aaaagatgca
     3121 gcaggagctg aacaagaaca tgcagaaaat gaaggaccag ctcaagcagc agggttcgaa
     3181 gagccagggc aagatcagca aggaccagtc gatgagcgaa caactcgcca agatggccgc
     3241 gcagcaggag tacatccgcc agcagttgca gcagctcaac caggaagaca acaaggacgg
     3301 ccgcaagtcg ctcggcaacc tcgaagacat cgccaaccag atggagcaga cggagaagga
     3361 catcgtcaac cggatgatct ccgaccagac cctgagacgg caggaagaga tcctgacccg
     3421 gctcctggaa agcgaacggg ccgagcgcga gcgggaccag gacgaacaac gcaaatccga
     3481 ggaggcgaaa aatcgcttcg agcgtaaccc tgcggccttc gaggagtata aaagacttaa
     3541 gttaaaggag atggaactgc tccggacggt acctccgtcg ctcagttcct tctaccggca
     3601 aaaagtttca gattacttcc aatccattga aaaatagcga actgctgatg cagggcaacg
     3661 accaattaca gaccctggag ctccggtccc agccggatag tattggcgcc gtggagcgca
     3721 tgatcgacca gctccgcgac cagtacgaag tcagcgagga catgtacggc aacatgctgg
     3781 tggccctcac cgaggctgtc accaacgcca tttaccacgg aaaccaatcg gatcccggta
     3841 aaaaggtgca catcaaatac ggccgcgtca ataactccct gaatttcacg atctccgacg
     3901 aaggccgcgg cttcgactac tacaatctcc ccgacccgac cgcccccgaa aacctcgaaa
     3961 aagagtgtgg tcgcggcatt tttctcatga agcacctcac cgaccagctc attttctccg
     4021 aaaacggccg ggtggtcgaa c
//
LOCUS       DAOTON010000152         4037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17926_length_4037_cov_3.738071, whole genome shotgun sequence.
ACCESSION   DAOTON010000152 DAOTON010000000
VERSION     DAOTON010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17926_length_4037_cov_3.738071"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..488)
                     /locus_tag="P5292_04170"
     CDS             complement(102..488)
                     /locus_tag="P5292_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38354.1"
                     /translation="MESHKFQMHRSVDLYTRKNGKIVPESKMSLVEGEEMACWPYNRE
                     FFHAHGFTNYAAERNDRMGLFYCPRLDKYFPAKYTVIFQIPKDEQIGEMVMDGDIESV
                     VGDRCYEPDGHDENKFPSWLLALGIV"
     gene            complement(566..1198)
                     /locus_tag="P5292_04175"
     CDS             complement(566..1198)
                     /locus_tag="P5292_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38355.1"
                     /translation="MKKFIYALLLAFICSFSSYAAPPAPDKPISDESAIQQNQPDVIS
                     VMSVPFGKSRAEVKEILYQTQPTCKSAAPNEHSSPDAIQYRDIQFAGRYGCSASFEFV
                     YDQFCLAIIQIPVTNIARDYEKMKSDLSAKYGEPSEDVEHYSPPYEKGDGYLELALSK
                     EKADIHAWWHLPSSRIRLSMSENRIVLSYMDDERTKEYFKVLAKRRQGQL"
     gene            complement(1350..1721)
                     /locus_tag="P5292_04180"
     CDS             complement(1350..1721)
                     /locus_tag="P5292_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38356.1"
                     /translation="MSTHPFAPTLKNLRDSRNKDATEMKKVLDAAITLSKVYKNQMER
                     LKMIIKELNHQESVPSIVLTYPQKKHVHIENFVVAECNLNQTMVDRLTAVFKIKGIEL
                     KELLDTIEETLDLHGLNSEDL"
     gene            complement(1782..2171)
                     /locus_tag="P5292_04185"
     CDS             complement(1782..2171)
                     /locus_tag="P5292_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38357.1"
                     /translation="MSDTSLQFINKKVEFWKRMFSGYFYEWKIDRYRQLIERIETGNY
                     HLDDPIELLAKIKALQEDEEVRQYYQFAPDSLVTHHDLYQAAKKLFRFRYERFAITGR
                     GKRMESKNATISDPTQPQLQIGVGIQH"
     gene            complement(2219..2809)
                     /locus_tag="P5292_04190"
     CDS             complement(2219..2809)
                     /locus_tag="P5292_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38358.1"
                     /translation="MHEYLTVFLISEWGSTMRFVFRMKDLIDLSFFMDPVKPGMKPDL
                     LQVMINQDNMKTPLEDVIKAQWPKLDPEAYKLNGKFRFSGKCGKIGDVRRTFKSCHYS
                     FIKARLHEMLIFPMEHLYSDWDLLSNFMGKTRSVAQMNACLNLGGPDIDSPEALKEYI
                     LLHNMNPSFAAWDHVRWIKFKQKNADDPEDYQPLQM"
     gene            complement(2985..3512)
                     /locus_tag="P5292_04195"
     CDS             complement(2985..3512)
                     /locus_tag="P5292_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38359.1"
                     /translation="MTQTTTQLRVLTWWDFESFNPCYDPQERYGDFSGTIIDILKDER
                     IPAKDRIWAATRRDILDDRTLRLFACKCLREVWHLLTDERSRKAVEVAERYAVGEATD
                     EELAAARTAARAAAWNAARTAARTAAWDAARTVAWDAAWSVAWDAARTAASQALEDAT
                     LKKEVTILIKLIEKP"
     gene            complement(3599..>4037)
                     /locus_tag="P5292_04200"
     CDS             complement(3599..>4037)
                     /locus_tag="P5292_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38360.1"
                     /translation="MDRASNSNVVYRRICYVGQQLSTGDVLLKDLSWRVDNVEFFIDR
                     DVAYFYHNPQTEEVQCICLHCEAHNKGKIVHHQVSKIKLGPTGLVSKAYLKKYVREQN
                     KSYLALQGKLRNGVFELKKHEIPKGIITVSYIPKVGNTIWIWL"
BASE COUNT          904 a          936 c          974 g         1223 t
ORIGIN      
        1 atttccctgg tgaaatcgta gatgattctc ttggttttca tgattgaaaa aaagagaaag
       61 ccggcatagc cggctctctt cgcagaacac actactccac ctcacacaat tcccagtgcc
      121 aaaagccagc tgggaaactt attttcatcg tgtccgtccg gctcgtagca ccgatcacca
      181 acaacgcttt ctatgtctcc atccatcacc atttctccga tttgctcatc tttcgggatc
      241 tgaaaaatga ccgtgtactt tgccggaaaa tacttgtcaa gccgcgggca gtaaaacagg
      301 cccattcggt cgtttctttc cgctgcataa ttggtgaatc cgtgtgcatg aaagaactcg
      361 cggttgtaag gccagcaagc catctcttcc ccctcaacaa gactcatctt gctttcaggt
      421 acaatcttgc catttttgcg cgtgtataga tctacgcttc tgtgcatttg aaatttgtga
      481 ctttccatga ttgtaaaaaa gaaaaacccc gtccagtgac ggggtttcct attgtccaac
      541 tattttttgt tgatcttatg aggggttaca attgcccctg ccgtcgcttg gcgaggactt
      601 tgaagtattc cttggtcctt tcatcgtcca tgtacgacaa gacaatacgg ttctcggaca
      661 tggacagacg aattcgactc gacggtagat gccaccaggc atggatgtcg gccttttctt
      721 tagatagcgc aagttcaaga tagccgtcac ctttttcata gggcggacta taatgttcta
      781 cgtcttcgga tggctcccca tacttagcag ataggtcgga cttcatcttt tcatagtccc
      841 tggcgatatt ggtaaccgga atttgaatga tggccagaca gaactgatcg tatacgaatt
      901 cgaagcttgc tgagcacccg tatctcccgg caaattggat atcccggtac tggatcgcgt
      961 caggcgaact gtgttcattc ggagccgcag atttgcaggt cggttgcgtc tggtacagga
     1021 tttcctttac ctctgccctg gacttgccaa agggcacgga catgacgctg atcacgtcgg
     1081 gctggttctg ctgaatcgcg ctttcatcgg aaatgggttt gtcgggtgcg ggcggcgccg
     1141 cataggaact gaagctgcaa atgaacgcca gcagcaaggc gtagatgaac tttttcatcg
     1201 gtttttgggt tttggaattg tgagtttttc cggcactaaa acatcgttga ccccactgcc
     1261 ggctgatggg attacgataa tattcttgtt cttcttttcc aagtatggaa tgctggacgg
     1321 ccagttaaag cggagatacg gttgagattt cataagtctt cgctgttaag tccatgcaaa
     1381 tctaatgttt cctcgatagt atccaaaagc tccttaagct ctattccctt aattttgaac
     1441 actgccgtta accggtcgac catggtttga ttcagattgc attcagctac cacaaaattc
     1501 tcaatgtgaa cgtgtttctt ttgtggatag gtaagcacta tgcttggaac gctttcttga
     1561 tgattaagct ctttgatgat catcttcagt ctttccattt ggttcttgta caccttggaa
     1621 agcgtaatgg ctgcatccag gacttttttc atttcagtag catccttgtt tctcgagtca
     1681 cgtaagtttt tcagcgttgg ggcaaaggga tgagtgctca tggttgacca aaagaaaatt
     1741 caggttggtt aggcataatg cttaccagaa tttgactcga gtcagtgttg tattccaact
     1801 ccaatctgca gctggggctg tgtaggatct gatattgtag cgtttttgct ttccatgcgc
     1861 tttcctctgc ctgtaatcgc aaatcgttca tatctgaatc gaaagagctt tttagctgct
     1921 tgatatagat catggtgagt aacaagacta tcaggagcaa attggtagta ttgacgtact
     1981 tcttcatctt cttgaagcgc tttgattttt gctaatagtt cgattgggtc gtccagatgg
     2041 tagtttccgg tttcaatgcg ttcaataagc tgcctgtacc ggtcaatctt ccattcgtaa
     2101 aagtatccgg aaaacatcct tttccagaat tccacctttt tgttgatgaa ttggaggctg
     2161 gtgtctgaca tgattgtaaa aaagagaaac cctggcttaa tgccaaggtt tttagtactc
     2221 acatctgcag cggttgataa tcttccggat catccgcatt tttctgcttg aacttgatcc
     2281 agcgaacgtg gtcccaggca gcgaaactgg gattcatgtt gtgcagcagg atgtattcct
     2341 tcagcgcttc cggagaatcg atatccgggc ctcccaggtt cagacaggcg ttcatctggg
     2401 ccacagatcg ggtctttccc atgaagttgc tgagcaggtc ccaatcgctg taaaggtgct
     2461 ccataggaaa gatcagcatt tcgtgtaacc gggctttgat gaatgagtaa tggcaggact
     2521 tgaaggtgcg tcttacatcg cctattttgc cgcattttcc ggaaaacctg aatttgccgt
     2581 tgagcttata ggcttccggg tctagcttgg gccattgagc tttgatgaca tcttccagag
     2641 gggttttcat attgtcctgg ttgatcatca cctgcagaag atccggcttc atcccgggct
     2701 ttacgggatc cataaaaaag gacagatcaa tcagatcttt catccggaat acgaatcgca
     2761 tggtggaacc ccattcgctg ataagaaata cggtgaggta ttcatgcatt ttggatcctg
     2821 gacaaaactt aagcaggttc gattcgaact gcttttcgac cttatgtcgg tcaaattgtt
     2881 gttttacggt ctgattttcc atgttgaata ggttatacaa aagaaaaacc ccaacggatg
     2941 accggagggg ttttgggctt aatttggata gacgatagct tggtttatgg tttttcgatt
     3001 aatttgatga ggatggtcac ttctttttta agtgttgcat cctctaatgc ctgcgatgct
     3061 gcggtccttg ctgcgtccca tgctacggac catgctgcgt cccatgctac ggtccttgct
     3121 gcgtcccatg ctgcggtcct tgctgcggtc cttgctgcgt tccatgctgc ggcccttgct
     3181 gcagtccttg ctgcggccaa ctcttcatct gttgcctcac ctactgcata ccgttcagcc
     3241 acttcaacgg ccttgcggct tcgctcatcg gtaagcagat gccacacctc ccggaggcac
     3301 ttacacgcga acaggcgcaa ggtgcggtcg tcaagtatgt ctctgcgcgt agccgcccag
     3361 atgcggtctt ttgccgggat gcgctcgtct ttcaggatgt caatgatggt acctgaaaag
     3421 tcgccgtagc gctcttgcgg gtcgtaacaa gggttgaaac tttcgaagtc ccaccatgtg
     3481 aggacgcgta gttgtgttgt ggtttgggtc ataggtttgg ttttttatgc tttttatgat
     3541 tgctgcataa gccggtcgta cagcgtggct gaaaccgtta tagggtggtc gtagcgtatc
     3601 atagccatat ccagatggtg ttgcctactt tggggatgta ggatacggta atgatgcctt
     3661 tagggatttc atgcttttta agctcaaata caccattgcg tagtttcccc tgtagggcca
     3721 aatagctttt gttttgctcg cggacgtatt ttttgaggta ggctttactt accaagccgg
     3781 tcggccccag tttgatcttt gaaacttggt gatgcacgat cttgcccttg ttgtgcgcct
     3841 cgcagtgtag gcagatgcac tggacttctt ctgtttgggg gttgtggtaa aaatacgcca
     3901 catcccgatc gatgaaaaac tcgacgttgt ccacgcgcca ggataagtct ttgagtagca
     3961 cgtcacctgt tgataactgc tgcccgacgt aacagatccg ccggtaaacc acatttgaat
     4021 tgcttgctcg atccatc
//
LOCUS       DAOTON010000153         4035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17935_length_4035_cov_3.517839, whole genome shotgun sequence.
ACCESSION   DAOTON010000153 DAOTON010000000
VERSION     DAOTON010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17935_length_4035_cov_3.517839"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /gene="nhaA"
                     /locus_tag="P5292_04205"
     CDS             <1..877
                     /gene="nhaA"
                     /locus_tag="P5292_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRS38361.1"
                     /translation="PVMAAIGGMIAPALFYTAFNAGTPSVSGWGIPMATDIAFALGVL
                     SLLGNRVPLSLKVFLTALAVVDDLGAVGVIAVFYTHDLSTTYLSYAAAVLLLLMIMNR
                     SGINSLWKYLIPGLVLWYCVLQSGVHATVAGVLLALCIPHKTRKGPPLLSRLEERLHT
                     PVNYFIMPLFALANTALPIKSEMVTHLSDPIALGVIFGLVVGKPVGIFLFSWLSVTTG
                     LSQLPAGTRFRELAGLGMLGGIGFTMAIFIALLAFRDPGQIVEAKIAILVASLLSGIF
                     GYLFLSKSLHERAGS"
     gene            858..1526
                     /locus_tag="P5292_04210"
     CDS             858..1526
                     /locus_tag="P5292_04210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS38362.1"
                     /translation="MKELDPEALASQLRSPHGDHAKAVGEFMSKGNKRLYALLFRRMT
                     ENPIGSVLEVGCGNGWYMDQFLALGNATTYQGLDLSEEMVRDAHERIKALGKTDLASV
                     QQGDILHWQSDVQYDLVIAMNLIYFFPEPSELIKRLSGFLKPGGQLLLGFRTAGSMQQ
                     LPFASFGFRLFEPNEVEDLFKGTGLTSIDTQLEREELETPAGTFSLVNAVVSGIRPSV
                     RALD"
     gene            1558..2022
                     /locus_tag="P5292_04215"
     CDS             1558..2022
                     /locus_tag="P5292_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38363.1"
                     /translation="MRDYKKYSFRNLAVATSFLWLFAAQVAWSQKVTKVTITDQLSAA
                     SALSYGTPKPAAAKDKFVLAGDKGAKVLVYLSLEPDSMMQPGFGFKYTVNRSQDGKEE
                     WIDERLLALKPTARYSMAAFTLTEPGRYHFLVTDQQDERKVYAEGWATVVQE"
     gene            2112..2753
                     /gene="msrA"
                     /locus_tag="P5292_04220"
     CDS             2112..2753
                     /gene="msrA"
                     /locus_tag="P5292_04220"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRS38364.1"
                     /translation="MRTLILVTYILFSLAACGQRNTPSPVMSDVTSVPPEFLDTATLG
                     GGCFWCTEAQYKLLDGVVNVVSGYSGGEIRNPAYKEVCSGRTGHAEVIQVVYDTRKLS
                     FDQILEAFWVSHDPTQLNRQGNDIGTQYRSVIFYHNAEQQRIAETYKKRLNDEQVFSD
                     PVVTEISAFKAFYPAEDYHQDYYAENGDQPYCQFVVAPKVEKFKKVFKERLKH"
     gene            complement(2762..3133)
                     /locus_tag="P5292_04225"
     CDS             complement(2762..3133)
                     /locus_tag="P5292_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRS38365.1"
                     /translation="MEKTRRLNKSEAGYHLLQLLSAVDGKFTLEEDLVIRKYLVENYP
                     FHVSLDGAIEHLSALHADDYVLHFQKCMDDFYSDSTRKERIEFLDFATQLVKADNEVT
                     GKENTFLKMLFNSWDSENEGL"
     gene            3274..>4035
                     /gene="mutL"
                     /locus_tag="P5292_04230"
     CDS             3274..>4035
                     /gene="mutL"
                     /locus_tag="P5292_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRS38366.1"
                     /translation="MPDLIRLLPDTVANQIAAGEVIQRPASAVKELLENAIDAGSSRI
                     DLFVKDSGKTLIQVTDNGCGMSATDARMAFERHATSKIRSADDLFHIRTMGFRGEALA
                     SIAAVAQVELKTRRHDDEVGTRLQIDGNEILSQEPVTAAAGSTFYIRNLFFNVPARRN
                     FLKSNPVEARHIIDEFERVALAQPSIAFSLNQNGLEVFKLPASNLRQRIVNLYGQPYN
                     ERLVPVQEETTLLNIQGFVGKPEFSRKTRGEQYFFV"
BASE COUNT          956 a         1029 c         1040 g         1010 t
ORIGIN      
        1 gccggtgatg gcagccattg gcggcatgat cgctcctgcc ttgttttata ctgcttttaa
       61 cgccggaacc ccgagtgttt cgggatgggg cattcccatg gcgaccgaca tcgccttcgc
      121 actgggagtc ttatcactgc ttggtaaccg cgtaccctta tccctgaagg tgtttctcac
      181 cgcactggct gttgtcgatg atctgggggc cgttggtgtg atcgccgttt tctatacaca
      241 cgatctttcg actacgtacc tgtcttatgc cgctgcagtg ctgttgttgc tgatgataat
      301 gaaccgaagc ggtatcaact ccctttggaa gtacctgatc cccggccttg ttttgtggta
      361 ttgtgtcctt caatccggcg tacatgcaac cgtcgccgga gtattgcttg ccctttgtat
      421 tcctcacaag acccggaaag gacctcccct cctgagtcga ctggaagagc ggctgcatac
      481 tcccgtcaat tactttatca tgccactatt cgccctggcg aataccgctt taccgatcaa
      541 gtcggagatg gtgacgcatc tttcggatcc gatagcgctt ggggtgattt tcggacttgt
      601 agtagggaag ccggtgggca tatttctgtt tagttggctt tcagtcacta ccggtctgag
      661 tcagttgcct gctggaacgc ggttccggga gttggccgga ttggggatgt tagggggaat
      721 tggtttcacc atggcaatat tcatcgcctt gctggccttt cgtgaccctg ggcagatcgt
      781 tgaagccaag atcgccatcc tggtagcgag cctgttatcc ggcatattcg gttatttgtt
      841 tttatccaaa agccttcatg aaagagctgg atcctgaagc gctcgcgagc cagcttcgct
      901 ccccgcatgg tgatcacgcc aaagcagtgg gagaattcat gagtaagggt aataagcggc
      961 tctacgcact cttatttcgc cggatgacgg agaacccgat cggcagcgtc ctggaggttg
     1021 ggtgtggaaa tggctggtac atggaccagt tcctggcatt agggaatgct acgacctatc
     1081 agggattgga tctaagtgag gaaatggtca gggatgccca cgaacggatc aaagcattgg
     1141 gaaagacgga tttggccagc gtgcaacaag gcgatatcct gcactggcaa tccgatgtgc
     1201 aatatgacct ggtcatcgcc atgaacctca tctatttctt ccctgaaccg tcggaactga
     1261 tcaagcgatt gtccggtttt ctaaagcccg gaggccagtt gctgcttgga ttccggacag
     1321 ccggaagtat gcaacaatta cccttcgcca gtttcgggtt tcgattattt gagccgaatg
     1381 aagtggaaga cttgttcaaa ggaacaggat tgacttccat cgacactcag ttggaacggg
     1441 aagaattgga gacacctgca ggtacctttt cgctcgtgaa tgcagttgtt tccggtataa
     1501 gaccgagcgt acgagcactt gactaaattg attttattca ttagatttga gtgtattatg
     1561 agagattata aaaagtattc atttagaaat ctggctgtag cgactagctt tctttggctc
     1621 ttcgctgctc aggtcgcctg gtcgcaaaag gtcaccaagg tgaccattac ggaccagctt
     1681 tcggccgcca gcgccctctc ctatggaact cccaaacctg ccgcagccaa ggataaattc
     1741 gtactggcag gcgacaaggg agcaaaggtg ctggtctatc tctcgcttga acccgactcc
     1801 atgatgcaac cgggcttcgg tttcaagtat accgttaacc gaagtcagga tgggaaagag
     1861 gagtggatag acgaacggct gttagcctta aaacccacgg ccaggtatag catggcggca
     1921 ttcaccctga cagaacccgg tcgatatcat ttcctggtaa ccgatcagca agacgaacgc
     1981 aaggtctacg cggaaggttg ggcgacggtg gtgcaggaat agggattcgg tcggttgtta
     2041 atctttcata aatcacgctc tccgacaaat cccaaacaat ccgaaccccg tggaagcgcg
     2101 tacatccgga catgcgaaca cttatcttgg tcacctatat attattcagt ctggctgcct
     2161 gcggccagcg caataccccc tccccggtta tgtctgatgt aacaagcgtg ccccccgaat
     2221 tcctggatac tgccacgctc gggggtggct gtttttggtg taccgaagct caatacaaac
     2281 tgcttgatgg agttgtgaac gtagtctccg gatattccgg cggagagatc aggaatccgg
     2341 cgtacaagga agtctgttcc ggaagaaccg gccacgcgga agtcatacaa gtggtttacg
     2401 acacgcgtaa attaagcttc gaccaaatac tggaagcgtt ctgggtatca cacgatccca
     2461 cccaactgaa ccgacaaggc aacgacatcg gaacgcaata tcgttcggtc attttctatc
     2521 ataatgcaga gcaacagcgt atcgcggaaa cctataagaa acgtttgaac gatgagcagg
     2581 tcttcagcga tccggtcgtc acggaaatat cggcattcaa ggctttttac cctgccgaag
     2641 attaccatca ggattactat gcggaaaacg gtgaccaacc gtattgccag tttgtagtcg
     2701 ctcccaaagt ggaaaaattc aaaaaggtct tcaaagagcg cttaaagcac taagcaactg
     2761 atcagagacc ttcgttttcg gagtcccagg agttgaagag catcttgagg aaggtatttt
     2821 ccttgccggt gacctcatta tccgccttca ccaattgggt ggcaaagtcc aggaactcga
     2881 tccgttcctt tcgggtggaa tcggaataga aatcatccat acacttttgg aagtgcagca
     2941 cataatcgtc ggcatgaaga gcgctcaggt gctcgatcgc gccatcgagt gaaacgtgga
     3001 aggggtaatt ctccaccagg tactttcgga tgaccaggtc ctcttccaac gtgaattttc
     3061 catcaacagc ggatagtaat tgcaagagat gatagcctgc ttccgattta ttcaaacggc
     3121 gtgttttttc cataaccaag tattcgctca tcaaattaga tttttttctc tgaataagca
     3181 acgtttcagg gcaccggttg ttttgaaatt tgggcttgtt tttcaacaac aaacgcgaaa
     3241 aacgtacctt taggcagatt tttgcatgac cgtatgcccg acttgatccg cctgcttccc
     3301 gacaccgtag ccaaccagat tgcagcaggt gaagtcattc aacgtcccgc ttccgcagtg
     3361 aaggaactgt tggaaaatgc aatcgatgcc ggcagttcca ggatcgatct cttcgtcaag
     3421 gattccggca agaccctgat ccaggtcacc gacaacggct gcggcatgag tgccaccgat
     3481 gcccggatgg cctttgagcg gcatgctacc tccaagatcc gctccgccga cgatctcttt
     3541 catatccgca cgatggggtt tcgcggtgaa gcccttgcct ccatcgctgc cgtggcgcag
     3601 gtcgaactga agacccgacg gcacgacgat gaagtgggta cccgtctcca gattgacggc
     3661 aacgaaatac tttcccagga gcctgtaacc gcagcggccg gtagcacctt ttacatccgg
     3721 aacttgttct tcaatgtgcc ggcaagaagg aatttcctca agtcgaatcc tgtcgaagcg
     3781 cgacacatca tcgacgagtt cgagcgggta gcgcttgctc agccgtctat cgcattttca
     3841 ctgaaccaga atggtctgga agtcttcaag cttcccgcat ccaatcttcg acaacggatt
     3901 gtcaacctct acgggcagcc ctataacgaa cgcctggtac cggttcagga ggagaccacc
     3961 cttctgaaca ttcagggttt tgttggtaag cccgagtttt cgcgtaagac ccgcggagag
     4021 cagtatttct ttgtc
//
LOCUS       DAOTON010000154         4028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_17977_length_4028_cov_3.420337, whole genome shotgun sequence.
ACCESSION   DAOTON010000154 DAOTON010000000
VERSION     DAOTON010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17977_length_4028_cov_3.420337"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2608
                     /locus_tag="P5292_04235"
     CDS             <1..2608
                     /locus_tag="P5292_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38367.1"
                     /translation="MDPAIGAIPPMIREKELAFAATLPDDRSAQARRAGINGDWNFRG
                     PWNVGGRTRALAIDVTNENVLLAGGVSGGMWRSTDGGNSWSRVSDGMGHPGVNAIAQD
                     TRPGREQTWYYLSGEAYGTSASGGSAFYLGNGMYKSGDGGLTWASLSSTVSGTPHVFD
                     NVWDVTWNVVTDPGNLIQDVVYGACFDAIYKSVNGGTSWSLVRGNAGVQSTYSYFTDV
                     AVSPTSVVYATLSSDGPQKGIWRAPGGTGFVNILPSNFPPNYDRLVIGIDPNNENIVY
                     FFGPTPGYGRMSTDFQGDTLWNSLWKYEYLSGNGTGTGGTWTDLSPNLPGNIGVFNGL
                     NTQGGYDLVVKVKPGNSNVVFIGGTNFFRSDNAFADSTQTTVIGGYALGAALPFVDEY
                     PGHHPDQHALVFLPSNPDIMYAGCDGGVSKTLDNTASNVVWEEKNLGYITSQFYTVTL
                     DHGGNSDVIIGGLQDNGTYYSNDTSATADWYHSFDGDGSYCQITDGGSYYYFSKQQGK
                     IAKTTLDPSGQVTAYRRIDPIGAENYQFINPFVLDPNNQDVMYLCAGQYIWRNDDLSG
                     IPLSNQWDSIATNWVRLPDTLPTAGEEITALAISRQPAHRLYYGTNKRHIYRFDSANT
                     GVPQRIDITYSLFPASGYTSCIAVNPQNADELLVVFSNYGVYSLFHSSDAGANWRKVG
                     GNLEQNTGGTGNGPSCRWASILPVSDGTVYLVGTSVGLFAADTLITNSTVWVRQGSTT
                     IGTAVVDMLDCRESDGLVAVATHGNGVYTARITSINDITTGTAFANRQPEFSLFPNPA
                     RDRITLQVASGTMSRPVHITLLDELGRELRSLSANSIDNLTLDVADLHAGLYYVRLQD
                     ELGTQQRAFIRQ"
     gene            3190..>4028
                     /locus_tag="P5292_04240"
     CDS             3190..>4028
                     /locus_tag="P5292_04240"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRS38368.1"
                     /translation="MYLNTHTYFSFLHGTLSVEELLAEAQRCGVRKLALTDINNTSGV
                     LDFIRLAPQYGVEPVVGIEFRRDHRLCYVGIARNNVGFHELNEFLTHCLRGGTNAEAD
                     VPDQPPLLPNVSWILVPPALPGAKALSDWIDERLRPIQPALRERVWFGLRRLPSWGGL
                     RIDPARLVLWWPVSFRSPGEHDIHRVLRAVDRNTLLTKLPPEDLADPGEVMLSPDALR
                     EQCTDSLSLIDNAERLLEGCGIAFDYHESKNKLSFTGSKALDRELLRRETFAGMRERY
                     GEAG"
BASE COUNT          815 a         1279 c         1072 g          862 t
ORIGIN      
        1 gatggatcct gccatcggtg cgatcccgcc catgatccgc gaaaaggaac tggccttcgc
       61 cgccaccctg cccgacgatc gctccgctca ggcgcgacgt gccggtatca acggcgactg
      121 gaacttccgt ggcccctgga acgtcggcgg acgaacccgt gcgctggcca tcgatgttac
      181 caatgagaat gtcttgctcg ccggcggcgt cagcggcggg atgtggcgca gcaccgacgg
      241 tggaaattcc tggtcgcgcg tcagtgatgg catgggtcat cccggcgtga acgcgatcgc
      301 gcaggatacc cggccgggcc gcgaacagac ctggtactac ctctccggcg aagcctacgg
      361 cacctcggcc agcggtggct cggccttcta tcttggcaac ggcatgtaca aatccggcga
      421 cggtggcctc acatgggctt cgttgtccag cacggtctcc ggtactccgc atgttttcga
      481 caacgtatgg gacgttacct ggaacgtggt caccgatccc gggaacctca tccaggacgt
      541 tgtttatggc gcctgcttcg atgctattta taagagtgtc aacggtggca cgagttggtc
      601 gctcgtgcgt ggcaatgccg gcgtacaatc cacttacagc tacttcaccg atgtggccgt
      661 ttcacccacc agtgtcgtct atgcaacact cagcagcgac ggtccgcaga aaggtatctg
      721 gcgcgctccc ggcggtacgg gcttcgtcaa catcctgcca tccaacttcc cgcccaacta
      781 cgaccgcctg gtgatcggca tcgatccgaa caacgagaac atcgtctact tcttcggccc
      841 cactcccggc tacggccgca tgagcaccga ctttcagggc gacacgctct ggaacagcct
      901 gtggaagtac gaatacctct ccggcaatgg cacaggaacg ggcgggacct ggaccgatct
      961 ctccccgaac ctccccggca acatcggggt cttcaacggc ttgaacacac aaggtggcta
     1021 tgacctcgtt gtgaaggtaa agccgggcaa ttcgaacgtt gtcttcatcg gcggtacgaa
     1081 cttcttccgc tcagacaatg cctttgccga tagcacgcag acgaccgtga taggcggcta
     1141 tgcacttggc gccgcgcttc cgttcgtcga tgaatacccg ggccatcatc ccgaccaaca
     1201 tgcgctggtc ttcctgcctt ccaaccccga tatcatgtat gctggttgtg acggaggtgt
     1261 ttccaaaaca ctggacaaca cggcttcgaa tgtcgtttgg gaggaaaaga acctcggcta
     1321 catcaccagc cagttctata cggtcacctt ggaccatggc ggtaacagcg atgtgatcat
     1381 cggcggactg caggataacg gcacctacta ttccaacgat accagcgcga cggccgactg
     1441 gtaccattcc ttcgacggag acggttccta ttgccagatc accgacggtg gatcgtatta
     1501 ttatttctcc aagcagcagg gaaagatcgc caagacgacg ttggatccgt ccggacaagt
     1561 gacagcctat cggcgtattg atccgatcgg tgcggaaaac tatcagttca tcaatccctt
     1621 cgtgctggac ccgaacaacc aggatgtgat gtacctctgc gccggtcagt acatctggcg
     1681 gaacgacgac ctctccggta ttccgctttc taaccagtgg gattccatcg ccaccaactg
     1741 ggtgcgactg cccgacacct taccgacggc aggggaggag atcaccgcgc tcgcgatctc
     1801 ccggcaacct gcacatcgtc tgtactatgg caccaacaag cggcatatct atcgcttcga
     1861 cagcgccaat acgggcgtac cgcagcgcat cgacatcacg tactcgctct ttcccgcaag
     1921 cggctatacg tcttgtatcg ccgtgaatcc gcagaatgcc gatgagttgc tggtggtgtt
     1981 ttccaactat ggcgtctata gcctctttca ttcgtccgat gccggcgcca actggcgaaa
     2041 ggtcggcggc aacctcgagc agaacaccgg cggaaccggc aacggacctt cctgccgttg
     2101 ggcaagtatt cttcccgtct ccgacggaac cgtatacctc gtcggtacgt cggtaggcct
     2161 ttttgccgca gatacgctca tcacgaacag caccgtctgg gtccggcagg gcagcacgac
     2221 catcggcact gcggtagtgg atatgctcga ctgccgcgag tcggacggcc tggtggcggt
     2281 cgctacgcac ggcaacggtg tttataccgc ccgcatcacc agcatcaacg acatcacgac
     2341 ggggaccgct ttcgccaatc gccagccgga gttttcactc ttcccgaatc ctgcacgcga
     2401 tcgtattaca ctgcaagttg cgagtggaac aatgtcgcgc cccgttcaca tcaccctgct
     2461 cgatgagctc ggccgcgagt tgcgttcgct atccgcgaat tcgatcgata accttacgtt
     2521 ggatgtagcg gatctccacg ccggccttta ttatgttcgc cttcaagacg agttgggtac
     2581 gcagcagcgt gcgttcatcc ggcagtaagg tttcaacaca ctgctcactc gcactgtttc
     2641 tcacactgtt tctaaacctg ctcaagctct ttctcacact tcgcactaac taaaaagtgc
     2701 gaagttcggg tgtcagtgca agtgtgtggt gttcccactg ctcactcgca ctgtttctca
     2761 cactgtttct aaatctgctc actcgcactg tttctcacac tacgcactaa aataaaagtg
     2821 cgaagtggga gaaacactgt gagtgaacag tggtatgccg ccccgcgttt ttttctctgc
     2881 cattggttgc ctgccaacca ggcgcgcgtt ttttatgctc tgaaaatcac aatgtcccgg
     2941 ttttttctct cgtctcgccc ttgcattttc attcgtgaaa gattactata caatcataca
     3001 aaatgattgt ttagcgatcc ggtagcaaat agtgaatagt aaacagtgaa cagtaaacag
     3061 taaacagtga tttagggaga ttaggtttcg ggccccgacg catcccccaa tccccaatca
     3121 ctaatctcgc cccacgtccc tcgccccacg tccctcgccc cacgtccctc gccccacgtc
     3181 cctccttaca tgtacctcaa cacccacacc tacttcagct tcctccacgg cacgctctcg
     3241 gtcgaagagc tgcttgcgga ggcgcagcgt tgcggggtgc ggaagctggc gctgaccgac
     3301 atcaacaaca cctcgggtgt gctcgacttt atccgactcg ctccgcagta cggcgtagag
     3361 ccggtggtcg ggatcgagtt ccggcgcgat caccggcttt gttatgtcgg catcgcgcgc
     3421 aacaacgtgg gctttcacga actcaacgag ttcctcacgc actgcctgcg cggcggtacc
     3481 aacgccgaag ccgatgtgcc cgatcagccg ccgctgttgc ccaacgtatc ctggatactt
     3541 gtgccgcccg cgctgccggg agcaaaggct ttgtccgact ggatcgacga acgcctgcgc
     3601 cccatccagc ccgccctgcg cgaacgcgtt tggttcggct tgcgccgcct gccttcctgg
     3661 ggcggtttgc gcatcgatcc tgcgcgcctc gtgctctggt ggcccgtaag cttccgctcg
     3721 cccggcgaac acgacatcca tcgcgtactg cgcgccgttg accggaatac gctcctgacc
     3781 aaactgcctc cggaagacct cgccgatccc ggcgaagtga tgctgtcgcc cgacgcactg
     3841 cgggagcaat gcactgatag tttgtcgctg atcgacaatg ccgaacggtt gctcgaaggc
     3901 tgcggcatcg ccttcgacta tcacgagagc aagaacaagc tcagcttcac cggcagcaag
     3961 gcgctcgatc gcgaactctt gcgacgcgaa accttcgccg gcatgcggga gcgctacggc
     4021 gaggccgg
//
LOCUS       DAOTON010000155         3968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18297_length_3968_cov_3.413493, whole genome shotgun sequence.
ACCESSION   DAOTON010000155 DAOTON010000000
VERSION     DAOTON010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18297_length_3968_cov_3.413493"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="P5292_04245"
     CDS             <1..1080
                     /locus_tag="P5292_04245"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38369.1"
                     /translation="LATCDVVDLDYRLCNYGTSPLTSATIDLTVNGTVQQSYNWTGSL
                     NTYNSEVLTFSGVTGTPGTTNAITFTVSNPNGNTDGNSANNSRNASFITYPLVGGPAV
                     NEAYAATAFPPSGWYLLNGGSSPTWTRSTAGLNGAGSAKMDFYNSPSGDQDILQLPAM
                     DLSGLSDAVLTFDLAHARYGSSNDNLKIKISTNCGTTWTTLYNKTGAALSTTAASTAS
                     FTPSNAAQWRNEIVSLSNYIGNDNVFIKFEANSNYGNNAYVDNVNVSFNTAVGTVTKT
                     ISFEVYPNPASSVASVDFTLDSKDDVTVEVFSKVGTLVYSNTAASLNAGDHSFSIPVE
                     GFAKGLYMVNIRTSAGSVMKKLVVE"
     gene            1145..1846
                     /locus_tag="P5292_04250"
     CDS             1145..1846
                     /locus_tag="P5292_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38370.1"
                     /translation="MKKLLLLASCFLLITGISGTRAQSLQITADELDLTGPANLTMTS
                     HIAISNIGSGTVSLVIERITNDLATGHSSYFCWGALCYPPTTNVSQQFMVNPGEGDTM
                     KAYLNPTGDVGTSHVTYRVYDADNPTDSASFTITYNATTVGVEELSLDKMLSPATPNP
                     ADQYTRLNYNVNSLNSKIVITNLLGGVVREIPLTDKKNTLLMPTGDLRAGIYLYSLIV
                     DNRTIGSRKLIVTHR"
     gene            1985..>3968
                     /locus_tag="P5292_04255"
     CDS             1985..>3968
                     /locus_tag="P5292_04255"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRS38371.1"
                     /translation="MPSKKLNQEALDYHAMGRPGKIEVIPSKPYSTQRDLSLAYSPGV
                     AEPCLRIADQPEDVYKYTAKGNLVAVISNGTAVLGLGNIGPEAAKPVMEGKGLLFKIF
                     ADIDVFDIEINETDIDRFVQTVKAIAPTFGGINLEDIKAPECFAIEQRLKKELNIPIM
                     HDDQHGTAIISGAALLNALELARKKIEDIRIVVSGAGASAISCAKLYLSLGARKENII
                     MFDSTGAIHSDRTDLDENKIFFANKTSVRTLDEAMKGADVFLGLSKGNIVSQDMVRSM
                     AKNAIVFALANPDPEITYPEALAAREDIIIATGRSDYPNQVNNVLGFPYIFRGALDVR
                     ATQINEAMKLAAVRAIAELAKDPVPEIVNLAYNQKNIVFGKDYIIPKPLDPRLITTVA
                     PAVARAAIESGVAQAPIANWNHYVENLQKRLGLDNKLIRVVTSKARQNPQRVVFAEAD
                     NYKILKAAQIVRDEGIAKPILLGNQERIRALLEENKLDLGDTPIIDPRREEEKRHEFG
                     EIFFRKRKRRGFTLYEAKKIMNERNYYGAMMVETGEADALISGLTRKYSDTIRPALQI
                     IGTQEDVKKVAGMYIMMTKKGPFFFADTTINVDPTAEDLAEITVLTAWAVQQFNVKPR
                     IALLSFSNFGAHDDPHAKKVRDAVAILHQKYPGMLVD"
BASE COUNT          965 a         1220 c         1001 g          782 t
ORIGIN      
        1 ctggcgacct gcgacgtggt tgacctcgac taccgcctgt gcaactacgg tacttccccg
       61 ctgaccagcg ccacgatcga tctcaccgtg aatggaacgg ttcaacagtc ttacaactgg
      121 accggtagtc tgaacaccta caactccgaa gtgctgacct tctccggtgt aaccggaacc
      181 ccgggcacca ccaatgcgat caccttcacc gtttcgaacc cgaacggaaa taccgacggc
      241 aactcggcca acaactcccg caacgcatcc ttcattacct acccgcttgt cggcggtccg
      301 gctgtgaacg aagcgtatgc agcaacggcg ttccctcctt caggctggta cctgctcaac
      361 ggtggcagct cgcctacatg gacccgcagc accgcaggcc tcaacggcgc aggttcggcg
      421 aagatggact tttacaactc gcccagcggt gaccaggaca ttctgcaact tccggccatg
      481 gacctcagcg gcctcagcga cgctgtcctg acgttcgacc ttgcacatgc ccgttacgga
      541 tcgtccaacg acaacctgaa gatcaagatc agcaccaact gcggtaccac ctggaccacc
      601 ctctacaaca agaccggcgc tgccctttct acgaccgcgg ccagcactgc ctccttcacg
      661 ccttcgaatg ccgctcagtg gcgtaacgaa atcgtgagcc tgagcaacta catcggcaac
      721 gacaacgtgt tcatcaagtt cgaagccaac tcgaactacg gtaacaacgc ctatgtcgac
      781 aatgtgaacg tctctttcaa caccgccgtt ggtacagtga ccaagaccat ctctttcgaa
      841 gtttacccga acccggcttc cagcgtggct tcggtcgact tcacgctcga cagcaaggac
      901 gatgtgaccg tggaggtatt ctccaaggtg ggcacgcttg tttacagcaa cactgccgcc
      961 agcctgaacg ccggcgacca ctccttcagc attccggtag aaggcttcgc gaaaggcctt
     1021 tacatggtga acatccgcac ctccgccggc tccgtgatga agaagctcgt agtggaataa
     1081 ttcttgctta aatcaacgga gccggatatc cggctccgtt gatttcttaa aaacactacc
     1141 ccgaatgaaa aaactgctac ttctcgcctc ctgtttcctg ctgatcaccg gaatttccgg
     1201 cactcgggca caatccctgc agatcactgc cgatgagttg gacctgaccg ggcctgcgaa
     1261 tctgaccatg acatcgcaca tcgcgatctc caatattggc agcggaacag tctcgcttgt
     1321 tatcgagcga atcacgaatg acctggcgac cggccactct tcttatttct gctggggcgc
     1381 gttgtgctac cctcctacta ccaacgtttc tcagcaattc atggtcaacc cgggtgaagg
     1441 ggacaccatg aaggcctacc tgaaccccac cggagatgtc ggcaccagtc acgtgaccta
     1501 tcgtgtgtac gatgccgaca atccaaccga cagcgcttcc ttcacgatta cgtacaatgc
     1561 cacgaccgtt ggcgtagaag aattgtcgct cgacaagatg ctgtctccgg ccacacccaa
     1621 tccggccgac cagtatacgc gcctgaacta caacgtcaat tcactgaaca gtaagatcgt
     1681 catcacgaat ctgctgggcg gagttgtacg cgagatcccg ttgaccgaca agaagaacac
     1741 cctcttgatg ccgaccggcg acctgcgtgc gggcatctac ctgtattccc tcatcgttga
     1801 caatcgaacg atcggttcgc gtaaactgat tgtaacccat cgttaacctt ctggaattcc
     1861 tggttaaaag ccggcaccaa cagtgccggc tttttttatg ctccaaccgc ggccgacggt
     1921 ttaggttggg atggatagtc gggattgata cctttgctgg ttcccactga aaccgaaggg
     1981 aaatatgcca agcaaaaaac tcaaccagga agcgttggat taccacgcga tgggacgccc
     2041 gggaaagatc gaagtaatcc cgagcaaacc ctacagcacc caacgcgacc tctccctcgc
     2101 ctattcgccc ggagtagcag aaccgtgcct gcgcatcgcc gatcaacccg aggatgtata
     2161 caagtacacc gccaaaggaa acctggtcgc ggtgatctct aatggcacgg ctgtgctggg
     2221 tctcggaaac atcggacccg aagcagccaa gccggtcatg gaaggaaaag ggctgctctt
     2281 taagatattc gccgacatcg atgtcttcga tatcgagatc aacgagaccg acatcgaccg
     2341 ttttgttcaa acggtaaagg ccattgcacc gacctttggt ggcatcaacc tcgaagacat
     2401 caaagcgcct gaatgcttcg cgattgaaca gcgtctgaaa aaagagctga acatcccgat
     2461 catgcacgat gaccagcatg gcaccgccat catttccggc gccgcgctgc tgaacgcact
     2521 cgaactggcc agaaaaaaga tcgaggatat ccggatcgtg gtgagcggtg ccggcgcctc
     2581 cgcgatctcc tgcgccaaac tttacctctc actcggggcc cggaaggaga acatcatcat
     2641 gttcgattcc accggcgcaa ttcacagcga tcgcaccgat ctcgacgaga ataaaatatt
     2701 cttcgccaac aagacttcgg tacgcaccct cgatgaagcc atgaaaggag cggacgtctt
     2761 tttggggctg tccaagggta acatcgtcag ccaggacatg gtccgttcca tggcgaagaa
     2821 tgcgatcgtc tttgcactgg ccaatccgga tccggagatc acctatccgg aagcacttgc
     2881 ggcacgggaa gatatcatca tcgccaccgg aagatccgat tatccgaacc aggtgaacaa
     2941 cgttctcgga ttcccttaca tcttccgcgg cgcactggat gtcagggcca cccagatcaa
     3001 cgaagcgatg aaactcgctg ctgtacgcgc catcgcggag ctcgccaaag atccggttcc
     3061 ggagatcgtt aacctggctt acaaccagaa gaatatcgtg ttcggcaagg attacatcat
     3121 tccaaaaccg ctggaccccc gtctcatcac gaccgttgca ccggctgtcg ctcgcgcagc
     3181 catcgaaagc ggtgtcgcgc aggcgccgat cgcgaactgg aaccactatg tcgagaacct
     3241 gcagaaacga ctggggctcg acaacaagct gatccgcgtc gttaccagta aggcccgcca
     3301 gaatccccag cgcgtggtct tcgcggaagc cgacaactac aagatcctca aagccgctca
     3361 gatcgtacgc gatgaaggga tcgccaagcc catcctcctt ggcaaccagg agcgcatccg
     3421 tgccttgctg gaagagaata agctggactt gggcgacacg ccgatcattg acccgcgccg
     3481 ggaggaagag aagcgtcacg aattcggtga gatctttttc cgcaaacgca agcgccgcgg
     3541 cttcacgctg tatgaggcga agaagatcat gaacgagcgc aactactacg gcgccatgat
     3601 ggtggagacc ggcgaagcgg acgcgctcat ttcgggtctc acgcgaaaat acagtgacac
     3661 gatccgtccg gccttgcaga tcatcggcac ccaggaggac gtgaaaaagg tagctggcat
     3721 gtacatcatg atgaccaaga agggtccgtt cttctttgcc gataccacca tcaacgtgga
     3781 tcccacggcg gaagacctgg cagagatcac ggtcctcaca gcctgggcag tccagcagtt
     3841 caacgtcaag ccgcggatcg ccttactttc cttctccaac ttcggcgcgc acgacgaccc
     3901 gcatgcgaag aaagtgcgtg acgcggtggc gatccttcat cagaaatatc ccggtatgct
     3961 cgtcgatg
//
LOCUS       DAOTON010000156         3957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18351_length_3957_cov_2.994618, whole genome shotgun sequence.
ACCESSION   DAOTON010000156 DAOTON010000000
VERSION     DAOTON010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18351_length_3957_cov_2.994618"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="P5292_04260"
     CDS             complement(<1..1129)
                     /locus_tag="P5292_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRS38372.1"
                     /translation="MQRLAFSCWMFLICICCCSGSLFAQTSNALNFDNTDDEVLVPNA
                     SARIAGSSQISLSFWVYPTNAAPAFPDYDGFAGIRNNVDADFYIVQLTDSQVEARFRN
                     SAGVNVDILYNGLLLNTWQHFVLVYNGAEFLLYHNGSLVGNTGATGTITNVAEALYIG
                     NLLFGTYSYWMQGSIDEVSLWNRALTPAEVRCIYRSSVDVTAIGLQLYYQFDQGIAGG
                     NNAGLTTLIDGMGNINGTLYNFALNGALSNWVEGADTRIPLSATLCRGGTYTFGTQTL
                     DEPGVYTETFAGTSGCDSTVALTLSWAIDTSLQQSGPMLTANQAGVAYTWVDCQNGYT
                     ALPGEIAQSFIALADGFYAVVLDDGTCVDTSSCYEVLVSGLS"
     gene            1273..2148
                     /locus_tag="P5292_04265"
     CDS             1273..2148
                     /locus_tag="P5292_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38373.1"
                     /translation="MKRFFTGFLLAFALTTLFSCEKTELETIGDNQAPPDQTVETVTL
                     ENYVTRAYILALGREPDSAEFIQQVNALKAANADSASRRAMVDAIFSDPDYLPRLYDQ
                     NRIDLLNNVDTAEYTGWINLFSLYLQDSTYLFQWPVLQYERDRMILLRDAFGELTTGA
                     IDVRELHRRMCNNYLYDQINMGSANFVISTFQHLIDRNPTASEQQSGVSMVDGNNAVL
                     FLQAGASKNDYLTIVTSARNYYEAQVVLLYRKYLQRDPGTVEMTTGTDRFLSTGDYTA
                     VQRDLLTTDEFIGIE"
     gene            2145..2633
                     /locus_tag="P5292_04270"
     CDS             2145..2633
                     /locus_tag="P5292_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38374.1"
                     /translation="MNLKRLLPLFLLPLALFACKKETDYQYEVDPVSVSAAGGDKTNQ
                     KTTVEFISIAYADLFGTNIPQTKLVNLNTAYTSFGDKKVVEDLIIRNFLSDTAAVIPA
                     ALSVSGDTLLFVTNTYKKFYNREPNPFEQYFWRELIRRDANLSPRTIYYALMTSDEYR
                     FY"
     gene            2641..>3957
                     /locus_tag="P5292_04275"
     CDS             2641..>3957
                     /locus_tag="P5292_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38375.1"
                     /translation="MKRRDFLRKSALATAGAFAAPYLLPGGRLFAASGLRRVNHVVFC
                     LYAGGVRNLESVHMNDGNLMPNLLNGNLPVSPDIAGAMEPLPTAPLPMPLQNYGTLFK
                     EFRYNSGPTGHYNGHTVAVTGSYVDVDLNLREHPRKPTIFEYYRKHNSPQQTAMNCWW
                     VANQLGPYPALNYSSSPDYGPAYGANFIAPTSLISPQGFDVLGNPRNFSSNEEATIRN
                     MKDFVNGVFNHSYTDGSAGVVNPEADANTLRQFIGDLYTKAQTPSWINSWNAGSAMNN
                     DMYNILFAEEIIRTFRPELLVVNMQDVDICHFNYTSYANNLRKADFAVAHLWKTIQET
                     PGMADDTVLIIAPEHGRNYYPNTSVDAYGRRATDHTAPTDPNFNGDPLMQMAREIFCL
                     VVGPPSVVRQGQVINSLMGESTEIVPTIASLLGFEDAMPGGFGVKPY"
BASE COUNT          831 a         1313 c         1082 g          731 t
ORIGIN      
        1 tggagagtcc gctgacgagt acttcgtagc aggaggaggt gtccacacag gtgccgtcgt
       61 cgagaactac ggcgtagaag ccgtcggcaa gggcgatgaa cgactgcgcg atctcgcccg
      121 gaagcgcggt gtaaccgttc tggcaatcca cccaggtgta ggccacaccc gcctggttgg
      181 cggtcagcat cggaccactt tgctgcagac tggtgtcgat cgcccagctc agcgtaagtg
      241 ctaccgtgga atcacaaccg gaggtgccgg cgaaggtctc ggtgtaaaca ccgggctcgt
      301 cgagggtctg cgtgccgaag gtataggtac cgccccggca caaggtcgcc gacaaaggaa
      361 tgcgtgtatc ggcgccttcc acccagttgg agagcgcgcc gttgagcgcg aagttgtaca
      421 gcgttccgtt gatgttcccc atgccgtcga tcagcgtggt caggccggcg ttgttaccgc
      481 cggcgatgcc ctggtcgaac tggtagtaca gttgtagccc gatcgcggtc acatccaccg
      541 agctccggta gatgcaacgc acttccgccg gcgtgagtgc gcggttccag agactgactt
      601 cgtcgatgct gccctgcatc cagtaactgt aggtgccgaa caacaggttg ccgatgtaca
      661 gggcctccgc gacattggtg atggttccgg ttgcgcccgt attgcccacc agcgatccgt
      721 tgtggtacag caggaactcc gccccgttgt acaccagcac gaaatgctgc caggtattca
      781 gcaacaaacc gttgtagagg atgtccacgt tgacgcccgc ggaattccgg aagcgcgcct
      841 ccacctgact gtcggtcaac tggacgatgt agaaatcggc atcgacattg ttgcggatcc
      901 ccgcgaagcc gtcgtagtcc ggaaaggcgg gcgcggcgtt ggtagggtac acccagaagc
      961 tgagcgaaat ctgactcgat cccgcgatgc gtgcgctggc attcgggacg agcacctcgt
     1021 cgtccgtatt gtcgaagttg agggcattgg aggtctgcgc gaagagactg ccggagcagc
     1081 aacagataca gatcaggaac atccaacagg agaaggccag gcgttgcatg ggaaggggtt
     1141 ttgtatcggg taaatttaat ttttttcgaa taacgcgccc gtcccccacg ttggtaatta
     1201 ccgggaaaag tcctgtgtta attcttcaaa gtcccgcttc atcaccgccc ttttctgata
     1261 attttacttc gaatgaaacg cttctttact ggtttcctcc tggccttcgc cctgaccacc
     1321 cttttctcct gtgagaaaac ggagttggag accatcggcg acaaccaggc tcctcccgac
     1381 cagacggtcg agacggtcac cctggagaac tacgtgaccc gcgcctacat cctcgccctc
     1441 gggcgcgaac ccgacagcgc cgagttcatc caacaggtga acgcgctcaa ggccgcgaac
     1501 gccgattcgg ccagccgccg ggcaatggtg gacgcgatct tctcggaccc ggactacctc
     1561 ccgcgcctct acgaccagaa ccgcattgac ctgctcaaca atgtcgatac cgccgagtac
     1621 acgggctgga tcaacctgtt cagcctgtac ctgcaggaca gcacgtatct ctttcaatgg
     1681 ccggtgctgc aatacgaacg cgaccggatg atcctgctgc gcgacgcgtt cggggaactg
     1741 accaccggcg cgatcgacgt ccgcgagctg caccggcgca tgtgcaacaa ctacctgtac
     1801 gaccagatca acatgggatc ggcgaacttc gtcatctcca ccttccagca cctgatcgac
     1861 cgcaacccga cggcttccga acaacaatcg ggcgtctcga tggtcgacgg caacaatgcc
     1921 gtgctcttcc tgcaggccgg cgcctcgaag aacgactacc tcacgatcgt cacctccgcg
     1981 cgtaactact acgaagccca ggtggtgctc ctctaccgca agtacctgca acgcgatccc
     2041 ggcaccgtcg aaatgactac cggcaccgac cgcttcctct ccaccggcga ctacaccgcc
     2101 gtacaacgcg acctcctcac caccgacgaa ttcatcggca tcgaatgaac ctgaaacgcc
     2161 tccttccgct cttcctcctg ccgctggcat tattcgcctg caagaaggaa accgactacc
     2221 agtacgaggt cgatccggtc agcgtcagcg cggccggcgg cgacaagacg aaccagaaga
     2281 cgaccgtcga gttcatctcc atcgcctacg ccgacctctt cggcaccaac atcccgcaga
     2341 cgaagctggt gaacctgaac acggcttaca cttcgttcgg cgacaagaag gtcgtcgagg
     2401 acctgatcat ccggaatttc ctcagcgata ccgcggccgt cattccggcg gcgctgtcgg
     2461 tgagcggcga cacgttattg ttcgtcacca atacctacaa gaaattctac aaccgcgaac
     2521 ccaatccgtt cgagcaatat ttctggcgcg agctgatccg ccgcgacgcg aacctctcgc
     2581 cccgcacgat ctactacgcg ctgatgacct ccgacgaata ccgcttctac tgagccgagc
     2641 atgaaacgac gcgacttcct tcgaaaatcc gcgctggcca ccgccggcgc cttcgccgcg
     2701 ccttacctcc tgccgggagg gcggctcttc gcggcctccg gcctgcgcag ggtgaaccac
     2761 gtggtcttct gcctctacgc cggcggcgtg cgcaacctcg agtcggtaca catgaacgac
     2821 ggcaacctga tgcccaacct gctgaacggc aacctgccgg tcagtccgga tatcgccggc
     2881 gcgatggaac ccctgcccac cgcgccgctg ccgatgcccc tgcagaacta cggcacgctc
     2941 ttcaaggagt tccgctacaa ttcggggccg accggacact acaacggcca tacggttgcc
     3001 gtcaccggca gctacgtgga cgtggacctc aacctgcgcg aacacccgcg caagcccacc
     3061 atcttcgaat actaccgcaa gcacaacagt ccgcagcaga ccgcgatgaa ctgctggtgg
     3121 gtcgccaacc agctcggccc ctacccggcg ctgaactact ccagctctcc ggattacggt
     3181 ccggcctacg gcgcgaactt catcgctccc acttcgctga tcagtccgca gggtttcgac
     3241 gtgctgggca acccgcgcaa cttctcgagc aacgaggaag cgacgatccg caacatgaag
     3301 gatttcgtca acggcgtctt caaccattcg tacaccgatg gttccgccgg cgttgtgaat
     3361 ccggaagccg acgcgaacac gctccggcag ttcatcggcg acctctacac gaaagcgcag
     3421 actccttcct ggatcaactc ctggaacgcc ggcagcgcga tgaacaacga catgtacaac
     3481 atcctcttcg ccgaggagat catccgcact ttccgtccgg agttgctggt ggtgaacatg
     3541 caggacgtcg acatctgcca cttcaactac acctcctacg cgaacaacct ccgcaaagcc
     3601 gacttcgccg tggcgcacct ctggaagacc atccaggaaa cgcccggcat ggccgacgat
     3661 accgtactca tcatcgcgcc ggagcacggc cgcaactatt acccgaacac ctccgtcgat
     3721 gcctacggtc gtcgcgcgac cgaccatacc gccccgaccg atccgaactt caacggcgac
     3781 ccgctgatgc agatggcccg cgagatcttc tgtctggtcg tcggaccgcc ttcggtggtg
     3841 cggcagggac aggtcatcaa ttcgctcatg ggcgaaagca cggagatcgt tcccacgatc
     3901 gcgagcctgc tcggcttcga agacgcgatg cccggcggct tcggtgttaa accctat
//
LOCUS       DAOTON010000157         3938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18459_length_3938_cov_2.840072, whole genome shotgun sequence.
ACCESSION   DAOTON010000157 DAOTON010000000
VERSION     DAOTON010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18459_length_3938_cov_2.840072"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="P5292_04280"
     CDS             complement(<1..225)
                     /locus_tag="P5292_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38376.1"
                     /translation="MTAFRYPLLAFYVLALFACNRSGQVESRELTFNDSIAPIIFRNC
                     SPCHRPGSAAPFSLLSYEDVKRHARTIQLTV"
     gene            complement(222..779)
                     /locus_tag="P5292_04285"
     CDS             complement(222..779)
                     /locus_tag="P5292_04285"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRS38377.1"
                     /translation="MRLLLIIGLWLPIWASAQSVFPLSLSDLKDHPATYRPAQDAKGV
                     AIVFLLPDCPSCQSYSKTLNDLYREFQPQGLEMIGVFPGKYASLSEVEVFRSDFRIAF
                     PLFVDKDQGQVRKVGAAVAPEVFLFDRQGHSVYSGRIDDWMVAPGKKRLQIRNHELRD
                     AMKALSEGRKVRVPRTIPIGCIIEQ"
     gene            complement(776..2221)
                     /locus_tag="P5292_04290"
     CDS             complement(776..2221)
                     /locus_tag="P5292_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRS38378.1"
                     /translation="MCLFIYKGGHFRIFGLLKTVRYAILDIETTGGNANTDRITEIAI
                     YLHDGERVVDEFVSLINPERRIPPFISRLTGIDDSMVAEAPRFFEVAKDIVKMTEGAV
                     VVAHNAQFDYSFIREEYRSLGFTFSRDYLCTVRLSRKLLPGHRSYSLGNLCERLGITI
                     ENRHRAAGDALATVRLFEMLIANDRNGDILKSVKNDYLHLRFPPNIDRSLLDKLPESP
                     GVYYLHNEDGSILYVGKSNNIRKRVLSHFVNKQNRKALELRNAIHDVTFTETGNELIA
                     LLLESDEIKKLQPRFNRAQRRTFFRFGIISETTEAGYFRLKPARVNAKDEPLVVCGSA
                     EEARTMLERLCRKFQLCQKLCGLYDIQHACFQHSIGQCNGACVGKESAEAYNERVESA
                     IASLRLQHPNFFLFGRGRTTGERSVVHVENGRYRGFGYADEGTGQQLDDLRSVITLRD
                     DNRDIQRILRQQLMNTLPRNLLIYQDELRYE"
     gene            complement(2265..3551)
                     /locus_tag="P5292_04295"
     CDS             complement(2265..3551)
                     /locus_tag="P5292_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38379.1"
                     /translation="MKRTITLLSFLLLVLAIPSKAQWTAMGSGLNGRIRSLVVYNNQV
                     YAGGTFTGHVAVWNGSTWSTAGDGLGTAATDSVNSLCVHGGVLYAGGVFTASGTGTTV
                     KYIAKLNTGTNKWEQVGSQINNFVRGLYSDGTDLYAGGAFTTPSSTVSRIAKLSGGTW
                     VQVGDPSALTGFVNTIVKFNGSLYIGGGFAGLVAKLNGSTWTTISGVTGTEVNALAVF
                     GNRIYIGGNFNQYLVTYNGSSIGTAFNTVNGRVWSLLALPTRIFAGGSFTSSPSTGAS
                     LPHFLLYDGASPFTALGSGFNGDVYAFTNFNGKIVAGGGFTTAGGVSATNVAISAQTI
                     DVPETDAVVAHAFYPNPMMDRSVFELETRETLVQPAVQIYDASGRLVRSLETDMEING
                     NQLRVIVLRDQLTAGYYLLSVSDNGRPVYAERFLVR"
     gene            complement(3621..>3938)
                     /locus_tag="P5292_04300"
     CDS             complement(3621..>3938)
                     /locus_tag="P5292_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38380.1"
                     /translation="SIEVNSPTLTISVYDNSFIDGDTLSLYFNGKCILDHYGVVKQKR
                     IIHLQLEPDINNYLILFAENLGLRPPNTAAILVNDGKTDRFFRLSSDMKVCSSLNFTY
                     NKK"
BASE COUNT          893 a         1012 c         1150 g          883 t
ORIGIN      
        1 gacggtcaac tggatcgttc gcgcgtgacg cttcacatcc tcataggaca gcagactgaa
       61 cggagcggcg gagcccggac gatggcatgg gctgcagttc ctgaagatga tgggtgcgat
      121 cgagtcgttg aaggtcagtt cccggctttc gacttgcccc gaccggttgc aggcaaacag
      181 cgccaggacg taaaaggcca atagggggta gcggaaagcg gtcattgttc gatgatgcat
      241 ccgatgggaa tcgtgcgtgg taccctgact tttcttcctt cactgagcgc tttcatggcg
      301 tcccggagtt cgtggtttcg gatctgcagt cgttttttcc cgggtgcgac catccagtcg
      361 tcgatccttc ccgaatagac ggaatgaccc tgacgatcga acaagaatac ttccggcgcg
      421 acggcagctc cgacctttct cacttgcccc tggtccttgt caacgaagag gggaaatgcg
      481 atccggaagt cgcttctgaa tacttccact tcggataagg aggcgtactt gccggggaat
      541 acaccgatca tttcaagtcc ctgcggctga aactcgcgat agagatcgtt cagtgttttg
      601 ctgtacgact ggcacgacgg gcaatcgggt aataaaaata caatagctac gcctttagca
      661 tcctgtgcgg gtcggtaagt ggcaggatga tccttcagat cggaaagcga caaaggaaac
      721 accgactgtg ccgaagccca aatcggaagc catagaccga tgatcaacaa caatctcatt
      781 cgtacctcaa ttcgtcctga tagatcagga gatttctggg caaggtgttc atcagttgtt
      841 gccgaaggat ccgttggatg tcccggttgt cgtcgcgcag ggtgatgacg gatcgaaggt
      901 cgtcgagttg ctgccccgtc ccttcatccg cgtagccgaa gccgcggtac cgaccgttct
      961 ccacatgcac caccgaacgc tcgccggttg ttcttccccg gccaaacagg aaaaagttag
     1021 gatgttgcag gcgaaggctc gcgatggcgg attcgacccg ttcattgtat gcctcggccg
     1081 attcttttcc tacacaggcg ccgttgcatt gaccgatgct gtgctggaag caggcgtgtt
     1141 ggatatcgta caagccgcag agtttctggc aaagctggaa cttccggcaa agccgttcca
     1201 acatggtgcg cgcttcttcc gccgatccgc aaaccaccag gggctcgtcc ttcgcattga
     1261 cgcgtgccgg cttcagccga aagtagccgg cctccgttgt ttcgctgatg atgccgaaac
     1321 ggaaaaaggt ccgtcgttgg gcacgattga aacgcggttg cagctttttg atctcatcgg
     1381 attccaacag gagcgcgatc agttcgttgc ccgtttcggt gaaggtcaca tcgtggatgg
     1441 cgttacgcaa ttcaagggct ttccggttct gcttgttcac gaaatgggag aggactcgct
     1501 tccgaatgtt gttactcttc ccgacgtaca ggatcgaccc gtcttcgttg tggaggtaat
     1561 agacgccggg tgattccggc aatttgtcca acaggctgcg gtcgatgttg ggagggaagc
     1621 gcaggtgcag gtaatcgttc ttgaccgatt tgagaatgtc gccgttccgg tcattcgcga
     1681 tgagcatttc aaagagccgg acggtggcga gggcatcccc ggcggcgcgg tgacggtttt
     1741 caatcgtgat gccgagtcgc tcgcagaggt tgcccaggct gtagctgcgg tgtccgggca
     1801 acagttttct gctcagccgg accgtacaga ggtaatcacg ggaaaaggtg aagcccagcg
     1861 agcggtactc ttcccggata aaggaataat cgaattgggc gttgtgggca accactaccg
     1921 cgccttccgt cattttcacg atatccttcg ccacttcgaa aaagcgtggc gcttccgcga
     1981 ccatcgaatc gtcgatgccg gtcaatcggg agatgaaagg aggaatcctt cgctctggat
     2041 tgatcaggga aacgaattca tccacgacgc gttcaccgtc gtgcaggtag atggcgatct
     2101 ctgtaatccg gtcggtgttg gcgtttccac cggtcgtttc aatatctaaa atggcgtagc
     2161 gcacggtctt cagcagcccg aagatacgaa agtggccgcc cttgtaaatg aaaaggcaca
     2221 ccgggcggtg tgctttttca ttgttgtgcc gtttacggca ggtattacct gaccaggaat
     2281 cgctcggcat agaccgggcg accattgtcc gataccgaaa gtaaatagta accggcggtg
     2341 agttggtcgc ggagtacgat cacccgaagc tggttcccgt tgatctccat gtcagtttcc
     2401 agagagcgga ccaggcgtcc ggacgcgtcg tagatctgga cggctggttg gacgagcgtt
     2461 tcacgcgtct cgagctcgaa tacggagcgg tccatcatcg ggttcggata gaacgcgtgc
     2521 gcaacgaccg catcggtttc cggaacatcg atcgtttggg cagagatggc cacgttggtg
     2581 gcggatacgc cgcctgctgt cgtgaaaccg ccacctgcta cgatctttcc attgaaattc
     2641 gtgaaggcat acacatctcc gttgaatcca ctgcctaagg cagtgaacgg gctggcgcca
     2701 tcatacaaga ggaagtgcgg aagtgaagca ccggtagaag gggaggaagt gaaggagcct
     2761 ccagcgaaga tccgcgtcgg gagggcgagc agcgaccaga cacgaccgtt gacggtgttg
     2821 aatgcggtgc cgatgctgga accgttatag gttacgaggt actggttgaa attaccaccg
     2881 atgtaaatgc ggttgccaaa tactgccagt gcattgactt ccgttccggt aacgccggaa
     2941 atggtggtcc aggtgctgcc gttcagcttt gcgaccaaac cggcaaaacc accgccgatg
     3001 tatagcgaac cattgaactt gacgatggta ttcacaaaac ccgtcaaggc gctcggatcg
     3061 ccgacctgta cccaggtgcc gccgcttaac ttggcgatac gagataccgt cgacgaagga
     3121 gtcgtgaagg cgccgcctgc atacaggtcc gtgccgtcac tgtagagtcc acgtacaaaa
     3181 ttgttgatct gcgaaccgac ttgttcccat ttgttggtgc cggtattcaa cttcgcgatg
     3241 tatttgaccg tagtgcccgt tcccgatgca gtgaaaacac cacctgcata gagtacacca
     3301 ccgtgaacgc aaaggctgtt gaccgaatcg gtagccgcag tgcccaggcc atcgccagcc
     3361 gtagaccagg tggagccatt ccagaccgca acgtgacccg tgaaggtgcc accggcataa
     3421 acctggttat tgtacacgac cagcgagcgg atgcggccgt ttaatccact gcccatagcg
     3481 gtccattgcg ccttggatgg gatcgccagg accaggagga ggaaagaaag taacgtaatg
     3541 gttcttttca tgataataag tgcggtttgg taaagcaggc gcaaaataag acaaagcggc
     3601 cgaagggccg gaggtcgccc ttattttttg ttataggtga aattgaggga gctgcaaacc
     3661 ttcatatcag aagaaagcct gaaaaaacgg tcggttttcc catcatttac caaaatggcg
     3721 gcggtattcg gcggacggag gcccagattt tccgcaaaga ggatcaggta attgttgata
     3781 tcgggttcca gttgcaagtg aatgatccgc ttttgcttca cgaccccgta gtggtccagg
     3841 atgcacttgc cgttgaaata gagtgaaagg gtgtcaccgt caatgaatga gttgtcgtaa
     3901 acggagatcg tcagggtggg agaattcact tcaatcga
//
LOCUS       DAOTON010000158         3936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18464_length_3936_cov_4.346045, whole genome shotgun sequence.
ACCESSION   DAOTON010000158 DAOTON010000000
VERSION     DAOTON010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18464_length_3936_cov_4.346045"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2313)
                     /locus_tag="P5292_04305"
     CDS             complement(<1..2313)
                     /locus_tag="P5292_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38381.1"
                     /translation="MRFSPDRLNARRFPWIGIMVAVLVVAGIASAIPARNAARVARSG
                     RIASDHVSLEMRWLDINNFKCPITNYGFIGNNNAENRAGGEWPAGSGEYYLYGGGIWI
                     AGTVDQGPFTENIGGNTVYTFRPSTTNPLGWQGLGAGSRAILTDPRAVIGYEPSGGTA
                     EMAPLTKVFMSTESDWPFPNTVSILDTYTEYDDQDPQRWNTGTGSNSGKDTGKWSGTP
                     TASTIATYGLGVKVKQTTYSWNYANNSDIHFVVYDVENIRTDGKTINNCFVGVVSDPD
                     IGNEAADDMAGFDASRNLGYAYDSDFSEAEFRGVPGFIGYRFLKSPVAEHDIDKNGDG
                     VIDNNPVLINSVPVYDVRAGQQIGLHAYKIFGRIAGDPDTEWEWYMTMAGHNFREDQN
                     QVYQPFDLSNTPEDQRFLQSTGPFTLKPGQPVQIVVAMMVAKAAGNPGDPISVRVAEL
                     QRVSDVAQSIFDNNFLLPVPPKAPALTARAGDRTAYLSWDTVAETTPDPFYSVASDPA
                     SALYDPNYKQYDFEGYRVWKSLTGRPDDWELLAEYDRQSVVPRNAEITVQAGVYGGTL
                     TYDAATTEGYFLEYGGTSLFNGHEFLVMLDGATQKMKVFDVSLGGQEIPCNTYADGDD
                     YWYYVGDNSQFELFDSNLNYLSSDSTRNANGINDQYVPGMIMYLAGMALQFDNVPAPA
                     STVIWRIVPSQEEDFGANTTVAHSYIDRNLINGQKYYYAVTAYDFQLSSPRSLESGRT
                     LSQAVVVPRSEPNGLATPDVAPVTRISGSSD"
     gene            complement(2366..>3936)
                     /locus_tag="P5292_04310"
     CDS             complement(2366..>3936)
                     /locus_tag="P5292_04310"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRS38382.1"
                     /translation="VTNLFFTSGNSRSANAVAGNNVVGQLHATSQINNNHELSGGIEI
                     TRSMTKQDRLSDPWSGTPFFDQWGPFNPIYGAAFLQDKIEYEGLTVTAGLRMDYYDPD
                     AYVWSDPLKPGAGSIMPDGSINVNGRPDVNADKVNAFRDKRVDPKMTLSPRLGISHPV
                     SDVGLVYFNYGHFVTQPANPYESYVPDLNRSNSRLGNPDLPFMRTISYEIGYSHEIVT
                     NTKLDVVLYRRDIRNTNTYQRIPAMNGAYTYAMASATTLVNGKEVNIGYGNSKGLELI
                     LTRYRGAEGMFSGWLSYSYLKAEAIYSDANDAYERFSRSALDPETGELAYPPDVPGIA
                     DYDRTHSFKANFDIRFRQGFGPQVAGMFPLQNMGINILEVINSGLPYTRINMSGKPVG
                     INNGFRKPWTYETDLMLNKQLRLANVPVTLYAKVENLFDTRNVLNIYERTGNPIDDGR
                     IMPQAQPNSVTPASPTWVYEGVKDGIDGSTPDGRISIAEDETAYRKAWSIYARDPLFF
                     SNPRIIRIGVGMSF"
BASE COUNT          728 a         1141 c         1182 g          885 t
ORIGIN      
        1 gtcgctcgac ccgctgatcc tggtgaccgg agcaacatca ggagtcgcaa ggccattggg
       61 ttcagaacgc ggcactacca ccgcctggct cagcgtccgt ccactttcga ggcttctcgg
      121 cgacgaaagc tggaagtcat acgccgtcac ggcgtagtag tacttctgtc cgttgatcag
      181 gttccggtcg atgtagctgt gggcaacggt ggtgttggca ccgaagtcct cttcctgtga
      241 cggcacgatg cgccagatca cggtgctggc aggagccggc acgttatcga actggagcgc
      301 catgccggcc agatacatga tcataccggg cacgtactgg tcgttgatgc cgttggcgtt
      361 cctcgtcgaa tccgagctga ggtagttcag gttactatca aacaactcga attgcgagtt
      421 atcgccgacg taataccagt aatcatcgcc atccgcgtaa gtgttgcagg ggatttcctg
      481 cccgccgagg ctgacgtcga agaccttcat tttctgggtg gcgccatcca gcatgacaag
      541 gaactcatgc ccgttgaaca ggctggttcc gccatactca aggaagtagc cttccgtcgt
      601 ggcggcatca tacgtgagtg ttccaccgta cacgcccgcc tgtaccgtaa tctcagcgtt
      661 tctcggcacc accgattgcc tgtcgtactc cgccagcagc tcccagtcat cgggacggcc
      721 tgtcaggctc ttccagacgc ggtagccttc aaagtcgtac tgcttgtaat tggggtcgta
      781 cagggcactc gccgggtccg acgcgaccga gtagaacggg tcgggagtcg tttcggcaac
      841 ggtatcccat gagagatacg cggtgcggtc tcccgcgcgc gccgtcaggg cgggagcctt
      901 tggcggaacg ggaaggagga agttgttgtc gaaaatggac tgcgcgacgt cggaaacgcg
      961 ctgaagctcc gccacgcgca cgctgatggg atcacccggg ttgccggcgg ctttcgccac
     1021 catcatcgca accacgattt gcaccggctg acccggtttc agcgtgaaag gtccggtgct
     1081 ctggaggaaa cgctgatctt ccggcgtgtt ggaaagatcg aacggctggt acacctggtt
     1141 ctggtcttcg cggaagttgt gccccgccat tgtcatgtac cattcccatt cggtatccgg
     1201 gtcgcccgcg atacgtccga agattttgta cgcgtgcaag ccgatctgct ggcctgcgcg
     1261 cacatcgtac accgggacgc tgttgatgag caccggattg ttgtcgataa cgccgtcgcc
     1321 gttcttgtca atgtcgtgct cggcaaccgg gctcttcagg aagcgatacc cgatgaagcc
     1381 gggaacgcca cggaactcag cctcggagaa gtcgctgtca tacgcatacc cgaggttgcg
     1441 cgacgcatca aaccctgcca tgtcgtccgc cgcctcgttc ccgatatccg ggtcggagac
     1501 aacaccgacg aagcagttgt tgattgtttt gccatcggtc cggatgttct ccacgtcata
     1561 caccacgaag tggatatccg agttgttggc gtagttccat gaatacgtcg tctgcttcac
     1621 cttcacgcca agcccgtagg tggcgattgt gctggcagtc ggcgtaccgg accatttgcc
     1681 ggtgtccttg ccggagttcg agccggttcc ggtgttccac cgctgcggat cctgatcgtc
     1741 gtactctgtg tacgtatcca gaatcgacac ggtgttcggg aacggccagt ccgactcggt
     1801 gctcatgaac accttcgtca atggggccat ttccgcggtt ccaccggagg gttcgtaccc
     1861 gatcaccgcg cggggatccg tgaggatggc acggctgcct gcgccaaggc cctgccaacc
     1921 gagcgggttc gttgtggacg gcctgaacgt gtagacggtg ttcccaccga tgttttcggt
     1981 gaagggaccc tggtctacgg ttccggcgat ccagatgccg ccgccgtaga ggtagtactc
     2041 acccgatccg gcgggccatt cgccaccggc gcggttctcg gcgttgttgt tgccgataaa
     2101 cccgtagttc gtaatcgggc atttgaaatt gttgatatcg agccatcgca tttcgagcga
     2161 cacatggtcg gacgcgatcc ttccggaccg cgccacgcga gctgcgttgc gcgcgggaat
     2221 ggcggatgcg attccagcga caaccagcac cgcgaccata atgccgatcc acgggaaccg
     2281 gcgtgcattc agccggtcag gcgagaatct catttggcac tccttcattc gtatatgttt
     2341 tcctgaaatc cgcatgtatc caccctcaga acgacatccc cacgccgatt cggatgatgc
     2401 gcggattcga gaagaacaag gggtcgcggg cgtagattga ccacgctttc cgatacgctg
     2461 tttcgtcttc ggcgatgctg attctgccgt cgggcgtgct gccgtcgatg ccgtccttca
     2521 cgccttcata cacccatgtc gggctggcag gtgtaacgga gttcggctgc gcctgaggca
     2581 tgattcttcc gtcgtcgatc gggttacccg tgcgctcgta gatgttcaac acgttgcggg
     2641 tatcgaacag gttctcgacc ttcgcgtaga gcgtaaccgg aacgttcgca agccggagct
     2701 gcttgttcag catgaggtcc gtctcgtagg tccacggttt ccggaacccg ttgttgatac
     2761 cgaccggttt gccgctcatg ttgatgcgcg tgtagggcaa tccggagttg atgacttcaa
     2821 ggatgttgat acccatgttc tgcaacggga acatcccggc cacctgcggc ccgaagccct
     2881 ggcggaaccg gatatcgaag ttcgccttga agctgtgggt gcgatcgtag tcggcgattc
     2941 cgggcacgtc aggcggatac gccagttcgc cggtttcggg gtcgagcgcg cttcgggaga
     3001 accgctcgta cgcgtcgttg gcgtcggagt aaatcgcttc cgccttgagg tacgagtagc
     3061 tcagccagcc gctgaacatc ccttcggctc cccggtatct cgtcaggata agttcgaggc
     3121 ccttggagtt gccgtacccg atgttgactt ccttgccatt caccagagtg gtggcggacg
     3181 ccatcgcgta ggtgtacgcg ccattcattg cgggaatgcg ctgataggtg ttcgtattcc
     3241 gtatatcgcg gcgatacaac accacgtcga gcttggtgtt ggtgacgatt tcgtgcgaat
     3301 agccgatttc gtacgaaatc gttcgcatga acggcaggtc cgggttcccg agacgggagt
     3361 tcgaccggtt gaggtctggc acgtagctct cgtacgggtt tgccggctgc gtgacgaaat
     3421 gaccgtagtt gaagtacaca agcccgacat ccgacaccgg atggctgatg cccagacgag
     3481 ggctgagcgt catcttcggg tctacgcgct tatcacggaa ggcgttcact ttgtccgcgt
     3541 tgacgtcggg ccgtccgttg acgttgatgc tgccatccgg catgatgctt cccgcgccgg
     3601 gtttcagcgg atcagaccag acgtacgcgt cgggatcgta gtaatccatg cgcaggccgg
     3661 ccgtaaccgt gagaccttcg tattcgatct tgtcctgcag gaaggccgca ccgtagatcg
     3721 gattgaaagg accccattga tcaaagaacg gggtgcccga ccacggatcg ctcagacggt
     3781 cctgtttggt catgctgcgc gtgatttcga taccaccact caattcgtgg ttgttattga
     3841 tctgtgaggt agcgtgcaac tgccctacaa cgttgtttcc agcaaccgcg ttagcgctgc
     3901 gggaattccc gctcgtaaag aacaggttcg tcacgc
//
LOCUS       DAOTON010000159         3928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18508_length_3928_cov_3.369481, whole genome shotgun sequence.
ACCESSION   DAOTON010000159 DAOTON010000000
VERSION     DAOTON010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18508_length_3928_cov_3.369481"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1927)
                     /locus_tag="P5292_04315"
     CDS             complement(236..1927)
                     /locus_tag="P5292_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38383.1"
                     /translation="MKKSLLALLFLTGSASAQWTSDPSLNTIVHDDAGLEEVVPLTTP
                     GDNGSTIISWFAANSSGNYDFKMQKLDVDGYGQWGATGKFISTYPQNSALFRYDLTSD
                     LFGNSVVAFQDMRLGILSVFAYRLGANGTLGWNANGIPCFDSLSTGGIAPDVAIAPGS
                     GNAFVAWNADDGNDRWISIQCIAPNGSFIWGNTPLRIKDSTGVVNYSRPQVVPMAGTD
                     DFLVFYVQETGNFPFTSTMFVQRYTAAGVAVWSNAVQVSTKTIPWVHYPSVIRDDADG
                     LYIGFNTSNPVMSTMNDVYVQHIDANGNLWNATGFEASTSTTTHKELVALRANMSPGV
                     HWLLMKELDSNQGQAGVTVQKFDNTGTMLLGANGASVTPISATYDEPWDMQEVTNGYI
                     IAYANGSFGNQELRAIRIDDNGATVWPSHVVMSNVASSKGDGSMLRFHSNNVVVVWDD
                     ERQDRGVYAQNIDIDGNLGVLTAIEASTQTPELQVAPIPLTTASNILLPRDSKLEQRL
                     ELFDMLGRPLGMLSVPATRQFVSIRELLSGDLPSSGTFLLRYTSGSVSIMRKLIR"
     gene            complement(1997..3073)
                     /locus_tag="P5292_04320"
     CDS             complement(1997..3073)
                     /locus_tag="P5292_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRS38384.1"
                     /translation="MKILDRYIIRKFLGTFFFSLALIILIAVIFDISEKIDDFIERKA
                     PFSKIVFDYYFNFIPYFANLFAPLFVFISVIYFTARMAANTEIVAILNSGISFRRMLR
                     PYFMAAGFLAALSFYFNGWVIPHSNKVKLEFENVYIKNPVEFKDRNIHRQISPGVYMY
                     MESYNNVDNIGYRFSLERIEDGKRVWFLNSDRIVWDSTASRWKIENYYIRRITGFRET
                     LTNGWQLDTTLSIKPADFRRRLNIVDAMDTPALSAFIEEEKISGSESINSYLVEKYRR
                     IALPFSTFILMLIGVSLSSRKVRGGIGSQLGLGITLSFAYILFMQVANTFAISGSIPA
                     MIAVWIPNIVFAFVAVYLLRVAPK"
     gene            complement(3392..>3928)
                     /locus_tag="P5292_04325"
     CDS             complement(3392..>3928)
                     /locus_tag="P5292_04325"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003007962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRS38385.1"
                     /translation="RDLRVQSAETIAAAGAEGNAIGGLSVGEPAEVMYEMTEVVNNIL
                     PKDKPRYLMGVGTPINILEGIDRGVDMFDCVMPTRNARNGMLFTRQGIINIRNEKWKD
                     DHGPIDPDGTSFVDQYYSRAYLRHLVVSKEILAAQIATLHNLAFYLDLVTEARQRILD
                     GSFKPWKESMVKVLGRRL"
BASE COUNT          867 a          970 c         1172 g          919 t
ORIGIN      
        1 cgatcactga tctcaccggg cgtatcgtac actcggaaat gcaacgcgta accccgggta
       61 cctcccgtta ctccatccgg gcaggcaagc tcgcacaggg tttgtatgta ttgcgcgtgt
      121 cgaacggaat cagcgaaaac caaaccacct ttggtgccgc agagtaacgg atcagtttcc
      181 tataaaaaaa ggccgcgaca tgttcgcggc cttttttatt tcgaatgaat agtggttatc
      241 ggatcaactt tctcataatc gaaaccgatc ccgatgtata acgcaacaag aaggtaccgg
      301 aggagggcag gtcgcccgat agcagttccc gaatggaaac gaactggcgg gtagcaggta
      361 cggatagcat tccgagcgga cgacccagca tatcgaacaa ttccagtcgt tgctccagct
      421 tgctgtctcg cggcaacaga atgttcgatg cagttgtcaa cggtatcggg gcgacttgta
      481 attccggagt ctgggtgctt gcttcgatcg cagtgagcac gcccaggttg ccgtcgatgt
      541 cgatgttctg cgcgtaaaca ccacggtcct ggcgttcatc gtcccatacg accactacat
      601 tgttggaatg gaagcgaagc atgctgccat cgcctttgct gctggcgaca ttgctcatca
      661 ccacgtgtga aggccaaacg gtagcgccgt tgtcgtcgat gcggatagcg cgaagctcct
      721 ggttgccgaa ggagccgttg gcataggcga tgatgtatcc gttggtgact tcctgcatat
      781 cccagggttc gtcgtaggtc gcgctgatcg gggtgacgga ggctccgttc gcgcctaaaa
      841 gcatcgtgcc ggtgttgtcg aacttttgga ccgtaacacc tgcctgaccc tggttgctgt
      901 cgagttcttt catcagcagc cagtgtactc ccggggacat gttagcacgc agtgcgacta
      961 gttccttgtg tgtcgtggtg gatgtgctgg cttcgaatcc ggttgcattc cagagattgc
     1021 cattggcatc gatgtgctga acatagacat cgttcatggt ggacatgacc gggttggagg
     1081 tattgaaacc gatgtagagt ccgtccgcgt cgtctcgtat tactgacggg tagtgaaccc
     1141 aaggaatggt cttcgtggaa acctgtaccg cgttcgacca gaccgcgaca cccgctgcgg
     1201 tatagcgttg gacgaacatc gtagaagtga aggggaagtt tccggtttcc tgtacgtaga
     1261 agaccaggaa atcgtcggtc cctgccatcg gaactacctg aggccggcta tagttcacca
     1321 cgccggtgct gtccttgatc cgcagcggcg tatttcccca gataaaggat ccgttcgggg
     1381 cgatgcactg aatggagatc caacggtcgt tgccgtcatc ggcattccag gctacgaagg
     1441 cgtttcccga gccgggagca attgctacat ccggtgcgat acccccggtt gaaaggctgt
     1501 cgaagcaggg aatgccgttt gcgttccatc ccaatgttcc gtttgcaccg agccggtagg
     1561 caaataccga aaggatgccc aggcgcatgt cctgaaaggc gacgacggag ttgccgaata
     1621 gatcgctcgt caggtcgtag cgaaagaggg cggagttctg tgggtaggta ctgatgaatt
     1681 ttccggtggc tccccactga ccgtagccgt cgacatccag cttttgcatc ttgaagtcgt
     1741 agttgccgga gctgttcgcc gcgaaccagg aaatgatggt ggagccatta tcacccggtg
     1801 ttgtgagcgg aacgacttcc tccaatcccg cgtcatcgtg gacaatggtg ttgaggctgg
     1861 ggtcggatgt ccattgtgcg ctggcgctgc cggtcaggaa aagcagggcg agtagcgatt
     1921 ttttcatggg tagtcggatt tgggtagccg aaaggtactc aaacccggac actcgaagcc
     1981 tgagaaaaat cagctgctat ttaggggcga cccgtagcag gtaaacggcg acgaaagcga
     2041 atacgatgtt cgggatccag accgcgatca tggccgggat cgagccactg atggcgaaag
     2101 tattcgccac ctgcatgaaa aggatgtaag cgaaactcag ggtaatcccc agcccaagtt
     2161 gagagccgat tcctccccgc accttccggc tggaaaggga aacgccgatc agcatcagga
     2221 taaacgtgga gaacggcagt gcgatgcgcc ggtatttctc gaccaggtac gaattgatgc
     2281 tttcggaacc gctgatcttt tcctcttcga tgaaagcact gagggccggt gtatccatcg
     2341 catcgacaat gttaagtcgc cggcggaagt ctgcaggctt tatcgataac gtagtgtcaa
     2401 gctgccatcc gttggtgagg gtttcccgaa atccggtgat tcggcggatg tagtagttct
     2461 cgattttcca tcgggaagcc gtgctgtccc acacgatccg gtcggagttc aggaaccaca
     2521 cgcgcttacc gtcttcgatg cgttcgagtg aaaagcggta gccgatgttg tcgacgttgt
     2581 tatagctttc catgtacatg tacacacccg gactgatctg ccgatggatg ttgcggtcct
     2641 tgaattcgac ggggttcttg atgtagacgt tctcgaactc gagtttcacc ttgttgctgt
     2701 gagggatcac ccagccgttg aagtagaacg agagcgcagc caggaaaccc gccgccatga
     2761 agtaggggcg gagcatgcgg cggaaggaga tgccggaatt caggatggcg acgatctccg
     2821 tattggcggc catgcgtgct gtgaagtaga tcaccgagat gaacacgaac agcggcgcga
     2881 acaggttggc gaagtagggg atgaagttga agtagtagtc gaatacgatc ttgctgaacg
     2941 gcgccttgcg ctcgatgaag tcgtcgatct tttcagagat atcgaaaatg accgcgatca
     3001 aaatgatcaa tgccagggag aagaagaacg tccccaggaa cttgcggatg atatagcggt
     3061 cgaggatttt caagaggggg gatcggactt aggggggctg tgttgggtta ttcagcgaat
     3121 agtgaatagc gaacagcgaa tagtttttgg tgaacagtga acagtgaata gtgaatagcg
     3181 gacagccaat agtaagttgt ggtttttcca aagcgctgag ggcaggaatt cactttcaaa
     3241 atttcggctg actactcgtg tcattattgc aaacatatgt gctgttttga aaaaacaatg
     3301 gattagttcg atttttatct ttagaaaaca acctaaaact attcgctgtt cgctgttcgc
     3361 tattcgctat tcgctattcg ctaatcactg ttcaaagtct ccgacccagc acctttacca
     3421 tactttcttt ccacggcttg aagctgccgt cgagaatgcg ttggcgggct tcggtgacga
     3481 ggtcgaggta gaaggcgagg ttgtgcagcg tggcgatctg ggcggcgagg atctccttgc
     3541 tcacgaccag gtgacgcagg taggcacggg aatagtactg gtcgacgaaa ctggtgccgt
     3601 cggggtcgat cggtccgtgg tcgtccttcc atttctcgtt gcgaatgttg atgatgccct
     3661 gccgggtgaa cagcatgccg ttgcgcgcgt tgcgggtggg catgacgcag tcgaacatgt
     3721 cgacgccccg gtcgatgcct tccaggatgt tgatcggcgt gccgacgccc atcaggtagc
     3781 ggggtttgtc tttcggcagg atgttgttga ccacttcggt catctcgtac atcacttcgg
     3841 cgggttctcc gaccgacaag ccgccgatgg cattgccttc ggctccggcg gcggcgatgg
     3901 tctctgctga ctggacgcgc aggtcgcg
//
LOCUS       DAOTON010000160         3914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18593_length_3914_cov_2.772221, whole genome shotgun sequence.
ACCESSION   DAOTON010000160 DAOTON010000000
VERSION     DAOTON010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18593_length_3914_cov_2.772221"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1318)
                     /gene="thrC"
                     /locus_tag="P5292_04330"
     CDS             complement(8..1318)
                     /gene="thrC"
                     /locus_tag="P5292_04330"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRS38386.1"
                     /translation="MRYFSTKDPNHPFSLRDAVLLGLPPDNGLFMPERIPVLPQPMIE
                     AMRSMSLPEIASIISKAWFGDEIPASDLETMCQDAYPFDTPLVRLRDQTYMLELFHGP
                     TLAFKDVGARFMARLMAYLNKGESRKLTILVATSGDTGSAVASGFWNVPGIDVVILYP
                     SGKVSEWQEKQLTTWGNNITALEVDGAFDDCQRLVKEAFLDPELRGSLRLSSANSISI
                     ARLVPQIFYYFRAVAQLPTGSADPVFVVPSGNFGNLTAGILAWMMGLGVHRFVAATNS
                     NDVVPEYLYTGEFKPRASVSTMSNAMDVGNPSNFWRMTALFGNDLEAMRKMIFAATAD
                     EATTARLMRLCWDECHYLTEPHAAVGLYGWEQYVKQNGSLHPGIVLGTAHPSKFIEAV
                     EAISGRSPEVPAALAEVIRKEKQAVRMKAEFSELRRFLLEKSKI"
     gene            complement(1325..2263)
                     /locus_tag="P5292_04335"
     CDS             complement(1325..2263)
                     /locus_tag="P5292_04335"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRS38387.1"
                     /translation="MPKTSVTVFAPATVANLGSGFDVFGLAISGLGDSLRMEPNTYGG
                     IRIRSIKGDDGKLSTDPEQNTAGVALMALLEHLGVNRGFDLWLTKKMPMGSGMGSSAA
                     SAAAAVVAANHLLGSPCSKEELVYWAMQGERIASGAAHADNVAPSILGGITLVRTTDP
                     LEVLRLPVPSRLHVAILHPHLELPTSESRKALPRQIPLSDAVRQWANTASLVAGLYAK
                     DLSLIGRAMEDRVAEPARGPLIPDFAKVKSAALDAGALACTISGAGPSIVALCSSRPQ
                     ADRVGAAMREVYLEKNLSCSLYVSAVNRKGTVITKR"
     gene            complement(2421..>3914)
                     /locus_tag="P5292_04340"
     CDS             complement(2421..>3914)
                     /locus_tag="P5292_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRS38388.1"
                     /translation="MVGVAGISARLFSALSQSKVNVILITQGSSEHTITFAVRPGDAA
                     QAKAAIEEAFRFEIRDHLIEQVKVEEGLSVIAVIGENMKNTPGVSARLFGALGKNGIS
                     VVATAQGSSELNISTVIRKQDLGKAMNALHQAFFLSEVKTLSVFLVGTGLIGKTLLKQ
                     VAEHRDHLKQARSLEIVIAGVANSRKMYFAPDGISLKRLSETLDRSGEPMDIRLFVDR
                     MKSMNLPQTVFVDCTSNESVASVYGDLIASNISVVTPNKLANAGPYRQYRKLHELVRK
                     HHAKFLYETNVGAGLPVINTLQNLLTSGDRIRSIEAVLSGTLSYIFNTFREGIPFSEV
                     VKQAKELGYTEPDPRIDLSGTDVARKLLILARETGLPLESGDIQIKPLLPARLMKAKT
                     VGAFLSALPDIDAEMESLRARAAKKGCVLRFIARLENGRASIQLTEVDTKHPFYNLSG
                     SDNIISFRTDRYNQRPLVVKGPGAGAEVTAAGVFAEIISIGNYFGVE"
BASE COUNT          908 a         1077 c         1052 g          877 t
ORIGIN      
        1 gtgttggtta aatttttgac ttttcaagta aaaatcgccg taattcggaa aattccgcct
       61 tcatccggac agcttgtttc tcttttcgga ttacttccgc caaagcagcc gggacctccg
      121 gagaacgacc ggatatcgcc tctaccgcct caataaattt cgaagggtgc gcagtgccca
      181 gcacgatgcc gggatgcaga gagccatttt gcttaacgta ctgctcccaa ccatacaagc
      241 cgacagcggc atgcggctct gtgaggtaat gacattcatc ccagcacagg cgcataaggc
      301 gcgcggtcgt agcctcgtcg gcagtagcgg caaagatcat tttccgcatc gcctcaaggt
      361 cattaccgaa caaggctgtc atccgccaga agttgcttgg gttgccgacg tccatcgcgt
      421 tggacatggt cgaaacggaa gctcgtggct tgaactcccc ggtgtagagg tactccggga
      481 cgacatcgtt gctattcgtc gctgcaacaa aacgatgcac gcccaagccc atcatccagg
      541 caaggattcc tgcggtcagg ttgccgaaat tcccggatgg cacgacaaat acaggatcag
      601 ccgatccggt cggcagctgc gccactgccc ggaaataata aaaaatctgg ggaaccaggc
      661 gagcaatgct gatgctgttc gccgaagaga gtcgcaagga tccacgcaat tcaggatcga
      721 ggaaggcctc tttcaccaat cgttgacaat catcaaaagc cccgtccact tcaagggccg
      781 tgatattatt gccccaggtc gtcaactgct tctcctgcca ttcacttact tttcccgagg
      841 gataaagaat cacgacatcg attcccggaa cattccaaaa gccgctggct accgcgcttc
      901 cggtatcacc ggatgtggcg acgagaatcg ttagtttcct ggattcacct ttgttgagat
      961 aagccatcaa gcgggccatg aagcgcgcgc cgacatcctt gaaggccagc gttgggccat
     1021 gaaagagctc caacatataa gtttggtcgc gcaaccgtac cagcggagta tcgaacggat
     1081 atgcgtcctg gcacatggtc tcgagatccg acgcaggaat ttcgtcaccg aaccaggctt
     1141 tggatattat cgaagcgatc tccggcaacg acatggaacg catcgcttcg atcatcggct
     1201 gaggcaagac agggatccgt tcgggcatga agaggccatt gtcagggggc aatcccaaca
     1261 ataccgcatc ccggagactg aaaggatgat tcggatcctt cgtagaaaaa tagcgcatac
     1321 tcgttcaacg cttggttatg accgttccct tgcggttaac ggccgagaca tacagactgc
     1381 aggataagtt cttttccaga tagacctcac gcattgcggc gccgacccga tcggcttgtg
     1441 gccgactgct gcacagtgca acgatggaag ggcccgcccc actgatggtg caggcaagcg
     1501 cgccggcatc caacgcggcc gacttgacct tggcgaaatc gggaatgagt ggaccgcgag
     1561 caggttccgc cacacgatcc tccatcgccc gcccgatgag cgacaagtct ttcgcataca
     1621 agccggctac cagggaagcc gtatttgccc attgacgaac ggcatccgac aggggtatct
     1681 gcctgggtaa cgctttcctg gattcggatg tgggcaactc cagatgagga tgaaggatgg
     1741 caacgtgcag gcgggacggc acaggcaagc gaagcacttc caacggatcc gtagtgcgaa
     1801 cgagtgttat tcctcccaga atggaaggcg caacattgtc ggcatgcgca gcaccgctgg
     1861 cgatccgttc tccctgcatg gcccagtata ccagttcttc cttgctacaa ggagagccca
     1921 gcaagtgatt tgcagccacc acggcagccg cagcgctcgc cgcactcgat cccatcccac
     1981 tgcccatcgg cattttcttt gtcaaccaca ggtcaaagcc ccggtttacg ccaagatgtt
     2041 cgagcaatgc catcaaggct acgcccgccg tattttgttc cgggtcggtg ctgagttttc
     2101 cgtcatcgcc ctttatgctt cggatccgga tccccccata ggtgttgggt tccatgcgca
     2161 gcgaatcgcc caaacccgag atcgcaagtc cgaacacgtc aaatcccgaa cccaggttgg
     2221 caacagtagc aggagcgaaa acggtaacgg aagttttggg cataggcgga gtggctgggc
     2281 gatggaaagc gggaattagt aattagtaat tagtaattag taattaaaaa ttgaggttcc
     2341 agaataaaca tacgattcgc taaactattt actgtttact attcactatt cactattcac
     2401 tattcgctat tcgctattcg ctattcaacg ccaaaatagt tcccaatact gataatctca
     2461 gcaaacacac cggctgcggt gacttcggcg ccggcgcctg ggccttttac gaccaagggt
     2521 cgttgattgt agcgatcggt ccggaaagag atgatgttat cgcttcccga taaattgtag
     2581 aaagggtgtt tggtatccac ctcggtcaat tgtatgctgg ctcttccgtt ttcgagtcgg
     2641 gcgatgaaac gaagtacaca gcctttcttc gccgctcgtg cgcgaagcga ttccatttcg
     2701 gcatcgatat ccggcaaggc agacaggaac gctccaacgg ttttggcctt catcaggcga
     2761 gcgggtaaga ggggtttgat ttgaatgtca ccggattcga gcggtaaccc ggtttcgcgc
     2821 gccaggataa ggagttttcg ggctacatcg gtaccggaca aatcgattcg cgggtccggt
     2881 tcggtatacc ccagttcctt ggcttgcttg acaacttcgc tgaaggggat gccttcccgg
     2941 aaggtattga agatatagga aagggtaccc gacaatactg cctcgatcga acggatacga
     3001 tcaccactcg tgagcaggtt ttgcagggtg ttgatgaccg gcaggccggc tcctacgttg
     3061 gtttcgtaca agaacttcgc gtgatgcttc cgtaccagtt cgtgcagttt ccggtattga
     3121 cggtacggac cggcattcgc cagtttattc ggcgtaacca ccgagatgtt tgacgcgatc
     3181 aagtctccgt aaaccgaggc taccgattcg ttcgacgtac aatcgacgaa gaccgtttgc
     3241 gggaggttca tggatttcat ccggtcgaca aacaaccgga tatccatcgg ttcgcccgaa
     3301 cggtcaagcg tttcactcaa gcgtttaagt gaaatgccat ctggagcgaa gtacattttc
     3361 cggctgttgg cgacgcccgc gatcacgatc tccagggaac gggcctgctt caggtggtcg
     3421 cggtgttcgg ctacctgctt caggagtgtt ttaccgatga gtccggtgcc gaccaagaag
     3481 acggagagcg tcttgacttc cgacaggaag aatgcctggt gcaaggcgtt catcgcctta
     3541 cccagatcct gtttccggat gaccgttgaa atgttcagtt ccgaagaacc ctgggcggta
     3601 gctacaacgc tgataccgtt tttcccgagc gcgccgaaaa gccgggcgga gacgccgggt
     3661 gtgtttttca tgttctcacc gatcacggct atcacgctca ggccctcctc cactttcacc
     3721 tgctcaatga ggtggtcgcg gatctcaaat cgaaatgctt cctcgatagc ggcctttgcc
     3781 tgcgcggcat caccgggacg gaccgcgaag gtgatcgtat gttcggaact gccctgggtg
     3841 atcagaatga cattcacctt cgactgactc aaggcgctga ataagcgtgc cgatatgccg
     3901 gccaccccga ccat
//
LOCUS       DAOTON010000161         3908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18625_length_3908_cov_4.609395, whole genome shotgun sequence.
ACCESSION   DAOTON010000161 DAOTON010000000
VERSION     DAOTON010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18625_length_3908_cov_4.609395"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..717)
                     /locus_tag="P5292_04345"
     CDS             complement(64..717)
                     /locus_tag="P5292_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRS38389.1"
                     /translation="MSALHRKLRITPGQRLIAYHAPAEFKALLSPLPAGVTISNRLSQ
                     HVDALHWFVRNRKEMEADLARVRSLLKPGFLLWCYYPKGTSGIQTDLTRDKGWDRLLG
                     LDVQWLTLVSFNDTWSAFAVRGKTDADQKKHARRAVREIEHYSDPVTKTVKVPTELAV
                     LFKKEKEAKAVFNSLAYSHRREYVEWIVSAKKEETRITRANGTIDRLKKGRKNPRNL"
     gene            complement(826..1239)
                     /locus_tag="P5292_04350"
     CDS             complement(826..1239)
                     /locus_tag="P5292_04350"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRS38390.1"
                     /translation="MSQKFESSKAPEPVGLYPHARRVGDLLFLSGVGPRERGSKKIPG
                     VELDDKGAIVSYDIEQQCRSVFQNVRWILEDAGSSWEKIVDVTVFLTNMKDDFPIYNK
                     LWAEYFAENPPCRTTIEINCLPTPIAIELKVIATI"
     gene            1443..1634
                     /locus_tag="P5292_04355"
     CDS             1443..1634
                     /locus_tag="P5292_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRS38391.1"
                     /translation="MKNGKVKFFNNAKGFGFIVEEGTQQEIFVHVTGLKDQIRDNDNV
                     TFEVEQGRKGPNAVNVRLA"
     gene            complement(1682..2536)
                     /locus_tag="P5292_04360"
     CDS             complement(1682..2536)
                     /locus_tag="P5292_04360"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HRS38392.1"
                     /translation="MASRIGAVSATELTNWQAVSGGSINQAWQFRYRSHGFFVKFNHA
                     ARYPGMFEAEADGLKRLQSAGIRVPGVIAFGSSGAFTWLLQRLIVSGRRAVDFEEHFG
                     RLLASLHRHSADGFGHVPDNWIGSLPQRNANCDRWTDFFREQRIDPLLREAISLKLLP
                     PDAAQYAERLFSNLENLFPSEPPALLHGDLWSGNRMTGEDGLPVWIDPAVYYGFREMD
                     LAMTRLFGGFDRRWEDAYREIYPLTPGFEERVELCNLYPLLVHVVLFGGGYADDFLRC
                     LRYYSGVK"
     gene            complement(2560..3528)
                     /locus_tag="P5292_04365"
     CDS             complement(2560..3528)
                     /locus_tag="P5292_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRS38393.1"
                     /translation="METLQFSDRQDSVRFYEDRYAHGYMGHWSVFEKARLINLVRELQ
                     LPARGVALDFGCGRGIFTSVIREALPGWRIFGCDISAEAIAWAKQHQPDITFFVLGDK
                     AYDNLRADFIHSHHVLEHTFNAEITAREMAGYAAAACTMLHSFPCDHPGSLEYQLSEA
                     VKSGIDPENGKFFFEDTAHQRRLSRKKAEALFAGDGFKGVDASYANQRAGAVKWIAES
                     DLALVLNITDSRLARDATAAAFLKGWRRKLLFRWWCHFAATAFTPADRGRHYRLKRVI
                     QFLAFVSCFWFAIPVNRAMQRAALREWETDRKKENGSDLFLVLRRG"
     gene            complement(3566..>3908)
                     /locus_tag="P5292_04370"
     CDS             complement(3566..>3908)
                     /locus_tag="P5292_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS38394.1"
                     /translation="QALADQTGVGELLLEPEVFSPDNDGKDDVLQIHLQFPDAGRRAT
                     VAIFDPHGNEVARPLEYSLAGTDHVLTWNGVGANGRLVAEGVYIVWTESFDLDGHVSR
                     LKKAVVVLRKG"
BASE COUNT          940 a         1087 c         1030 g          851 t
ORIGIN      
        1 atgggggctt tcgctccgga ttgatattac cttcggcctt gatcaaaaaa acaaaagagc
       61 cgattaaaga ttacgcgggt tcttccgccc cttcttcagc cggtcgattg tgccgttggc
      121 gcgtgtaatg cgggtctcct ccttcttcgc cgatacgatc cattcgacgt attcgcgacg
      181 gtgtgaatag gcgagtgaat tgaaaaccgc tttcgcctct ttttcctttt taaatagtac
      241 agcgagttca gtgggcactt tcacggtttt tgtaaccggg tcggagtagt gctcgatttc
      301 gcgaaccgca cgccttgcat gcttcttttg atcggcatcc gtcttccccc gaaccgcgaa
      361 cgcgctccag gtatcgttga acgaaaccag cgttaaccac tgaacatcca atccgagcag
      421 acgatcccag cctttatcac gtgtcagatc ggtttgaatg ccggaagtgc ctttgggata
      481 gtaacaccac aacaggaaac cgggcttcag caaggaacgg accctggcaa ggtccgcttc
      541 catttccttc cggttgcgca cgaaccaatg taaggcgtca acatgttgcg aaaggcgatt
      601 ggaaatcgtc acacccgccg gtagcggcga gagcagggct ttgaattcag ccggagcgtg
      661 gtaggcgatc agtcgttgcc cgggcgtgat gcggagtttc cggtgtagag ccgacatgct
      721 ggtggggagt tgaaccttca aacgttgtaa cgttaaaacg tttgaacgtt ctaacgttat
      781 gggtatagtt aaggcaggta atgcgattcc tgcatcaggg ggcaatcaga tcgtcgcgat
      841 gacttttagt tcgatggcga tcggggttgg gaggcagttg atctcgatcg tcgtgcgaca
      901 gggcggattt tcggcgaagt attccgccca taatttgttg tagatgggga aatcgtcttt
      961 catgttcgtc aggaagacgg tcacatccac gatcttttcc caactacttc cggcatcttc
     1021 caggatccaa cgaacgtttt gaaacacgga acggcactgt tgctcgatat cgtaagagac
     1081 gatcgcgcct ttgtcgtcga gctcgacacc gggaattttt ttggaacccc gttcgcgcgg
     1141 accgacaccg gacaggaaga gcaagtcacc aacccgacgt gcatggggat acaatccgac
     1201 gggttcgggg gctttggaag attcaaactt ttgagacatg gcagacaggt gactacctgc
     1261 aaatctacag gaaaagccat ggattcattc cgggtaaagg ctcagaaagg gccggaaaac
     1321 agtgaaaaag cgtgattctg tttaaaaaat gcggtttaac gcgggcttaa cgcataaacg
     1381 ttaggtttcc cccggttatc gccgtacctt tgcagcaaca ccaattaaaa tcaatatcac
     1441 taatgaaaaa tggtaaagta aaattcttca acaacgccaa aggctttgga ttcatcgttg
     1501 aagaaggtac ccaacaggaa attttcgttc acgttaccgg tctgaaagac cagatccgtg
     1561 ataacgacaa tgtcacgttt gaagtggaac aaggtcgcaa aggcccgaac gcagttaacg
     1621 tccgccttgc gtaagcatag cgcctgagag aaccgaaaaa ccccgccctg tgcggggttt
     1681 tttattttac accggaataa tagcgcaagc aacgcaggaa atcatccgca tatccgccgc
     1741 cgaaaagcac cacatgcacc agcagcggat acaaattgca cagttcgacc cgctcttcaa
     1801 acccgggagt caagggataa atctcccggt aagcatcctc ccaccgacga tcgaatccgc
     1861 caaacaagcg ggtcatggcc agatccattt cgcgaaaccc atagtaaaca gccggatcga
     1921 tccatacggg caagccgtct tcgccggtca tccgattccc gctccacaga tcgccgtgca
     1981 acaaagcagg cggctccgat ggaaaaagat tttccaaatt cgaaaaaagt cgctccgcat
     2041 actgcgcggc gtccggggga agcagtttca aggagatcgc ttcgcgtaac aacgggtcaa
     2101 tgcgttgttc gcggaaaaag tcggtccaac gatcgcaatt cgcattccgc tgcggcagcg
     2161 agccgatcca gttatccgga acatgcccga aaccgtctgc gctatgccgg tgcagcgacg
     2221 cgagcaagcg accgaagtgt tcttcgaagt cgacggctcg tcttcctgaa acgatcagtc
     2281 gttgcaacaa ccaggtaaaa gcgccggaac tgccgaaagc gatcaccccg ggcacccgta
     2341 ttcccgccga ctgcaagcgt ttcaatccgt cggcttccgc ttcgaacatg ccgggatacc
     2401 tcgctgcatg attgaacttg acgaaaaaac catgtgaccg atagcgaaat tgccaggcct
     2461 ggttgatgga gcccccggaa acagcctgcc agtttgtaag ttcagtagcg cttactgcgc
     2521 cgatgcgact cgccaagtcc cgaacgatga cttcagggat catccgcgtc gcaacacaag
     2581 aaagaggtcg ctgccgtttt ccttcttcct gtccgtttcc cattcgcgca atgccgcgcg
     2641 ttgcatggcc cggttcacgg gaatggcgaa ccagaaacag gatacgaatg caagaaactg
     2701 gatcacccgc ttcaaccggt aatgtctgcc gcggtcggcg ggcgtgaagg cggtcgcggc
     2761 gaaatgacac caccagcgaa acagcaattt tcttctccag cctttcagaa atgcggcagc
     2821 agtggcatcc cgtgccagtc ttgaatcggt aatgttcagc actaacgcaa gatcgctttc
     2881 ggcgatccac ttgaccgcgc ccgcgcgttg attggcgtag gatgcatcga cgcccttgaa
     2941 tccgtcgccg gcaaacaagg cttccgcttt cttcctggag agtcgtcgct gatgcgcggt
     3001 gtcctcgaaa aagaacttgc cgttctccgg gtcgattccg cttttaaccg cttcactcag
     3061 ttgatactcc aggctgccgg gatgatcgca gggaaagctg tgtaacatcg tgcaagccgc
     3121 tgcagcatat ccggccattt cgcgggccgt gatctccgcg ttgaacgtat gttccagcac
     3181 gtgatggctg tgaatgaaat cggcgcgcag gttgtcgtat gccttatcgc ctaagacaaa
     3241 aaatgtgatg tcgggttggt gctgtttggc ccaggcgatg gcttcggcac tgatgtcgca
     3301 accgaagata cgccagccgg gaagcgcttc gcgtatcacg gaagtgaaaa tgccgcgacc
     3361 acatccgaaa tcgagcgcga ctcctcttgc cggaagctgg agctcgcgga ccaggttgat
     3421 gagccgcgcc ttttcaaaca cggaccaatg acccatgtag ccatgcgcgt accgatcctc
     3481 gtaaaagcgt accgaatcct gacggtcgga aaactgcagt gtttccatca actgcaggca
     3541 aagtaacgac cttcccccga aaccctcaac ctttcctgag caccaccacg gcttttttca
     3601 aacgactcac gtgtccgtcc aggtcgaacg attctgtcca gacaatgtac accccctctg
     3661 ccaccaagcg tccgttggcg ccgacgccgt tccaggtgag cacatgatcg gtacccgcca
     3721 gcgaatactc cagcggccgg gccacctcgt tgccatgcgg gtcgaagatg gccaccgtcg
     3781 cgcgtcgacc ggcatcgggg aactgcaagt gaatctgcaa cacgtcgtct ttcccgtcgt
     3841 tgtcgggtga aaatacctcc ggctccagca gcaattcgcc aacgccagtc tgatcagcga
     3901 gtgcttgt
//
LOCUS       DAOTON010000162         3907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18632_length_3907_cov_2.898494, whole genome shotgun sequence.
ACCESSION   DAOTON010000162 DAOTON010000000
VERSION     DAOTON010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18632_length_3907_cov_2.898494"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="P5292_04375"
     CDS             <1..477
                     /locus_tag="P5292_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38395.1"
                     /translation="GPDVTGCIGSTVTLDAGAGFASYLWNDNSTAQTLDVTVDGTYSV
                     LVTDNNGCENFDAVVVNFIQCQTVRPEITPVGGPGIDLFPNPGRNRVQLTVPGVKDTE
                     VTVIVRDILGNVMSFSRENAGHGYIKNLDIKGLAAGIYTVNVTYAGQNETIRLVKE"
     gene            712..2283
                     /locus_tag="P5292_04380"
     CDS             712..2283
                     /locus_tag="P5292_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38396.1"
                     /translation="MQHLFETTPRNLTLGLLALVVAAFTSSSPVNAEAPGALPLTGID
                     GTDRYSATVCAGSSICFDVFTQPDKDVQIRRWVGDLPNALFELLNAQGNSGHLCWTPG
                     EQDAREEPYRFTIFAGTENTEVPVEYTLLVPHIQIRSVTKEISCKGMDDGQILLESIP
                     AVDEYFYTWSDTPGQTPERDHLKAGSYLVVISDPNGCSIEREFVLDEPEALTTKYTVI
                     PSDCRIVNGRIKLEVSGGTWPYQYDWNGLSGSEPELGPVAEGYYGLALTDANGCSLRD
                     RIHVPSAALMVQNAKIQPASCPEQADGSASITINGGTAPYEIEWFPYGGSAQHAAALT
                     AGLYTVQVADAAGCSATLELSIEARSQSPKLEMPATIEFCQGSQVALSAGNAWHTVEW
                     SDGSRHSSLSVNRAGKYSVSVTDDHGCQQQSTIVATAVNCNDQRSARPTDSPLLRVSP
                     NPAGDLTTVLLDPEYPEGTTLFLSDALGRQLQVITPRRDGAEIEIPLRNYPAGTYLLT
                     ILQPSGSETIRLIKY"
     gene            2362..3099
                     /gene="gldF"
                     /locus_tag="P5292_04385"
     CDS             2362..3099
                     /gene="gldF"
                     /locus_tag="P5292_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HRS38397.1"
                     /translation="MYALFRKELSSFLGSLTGYLVMTVFLLSVGILLWIFPDTSFSIP
                     DNRIAALDGLFIVAPWLFLFLIPAISMRMFSEEQKTGTLELLLTRPLSEFQLVFAKFL
                     AAFVLVVLSLIPTLIYYFTVYRLAVPAGNVDTAGIAGSYIGLLFLSSGFVAIGIFCSA
                     QTNNQVVAFLFALILCYFFYAGFDAFALLGIPVRLQNILSQLGIANHYAAMSRGVMDS
                     RDLLYFVSLTTCFLLLARFTLEKRKWS"
     gene            3096..>3907
                     /locus_tag="P5292_04390"
     CDS             3096..>3907
                     /locus_tag="P5292_04390"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HRS38398.1"
                     /translation="MSSGKQRKDSLLRLIASLVILVSVNIIGNFLFFRIDLTAEKRYS
                     ISDASRELAGSLNDVVYFKVYLDGELPPGFRRLRNATRELLDEFRIYAGGNIEYEFVD
                     PSAEPEEKKRLELYKQLARKGLLPTNLEEREKGGSSQKIIFPGAIASFGGEEIPVQLL
                     KSRLGSSPEEMLNTSVENLEYEIAGVLRRFTRTGGAPRIGFLRGHGELDNKHISDAAR
                     ALNEYYNVDTVFIGGRLDALKDHRLLIIAKPTLSFDEKDKFVIDQFIMRGGRV"
BASE COUNT          897 a         1055 c         1012 g          943 t
ORIGIN      
        1 ggtccggatg tgaccggctg catcggttct acggttaccc tcgacgctgg tgccggtttc
       61 gcatcctatc tgtggaacga caactccacc gctcaaacgc tggatgtcac ggttgatggt
      121 acgtactccg tactcgtcac cgacaacaac ggctgcgaga acttcgacgc agtggtcgta
      181 aacttcatcc agtgccagac ggttcgtcct gaaatcaccc cggtgggtgg acctggcatc
      241 gacctcttcc cgaaccctgg ccgcaaccgt gtacaactga cggttccggg tgttaaggat
      301 acggaagtaa cggtcatcgt acgcgatatc ctgggtaacg tgatgtcctt ctcccgtgag
      361 aacgccggtc acggttacat caagaacctt gacatcaagg gcctcgctgc cggtatctat
      421 acggttaacg taacgtacgc cggtcagaac gagaccatcc gactcgtcaa agaataagtt
      481 gattgtagtg attagtcaag ggcgtaccgc aaggtgcgcc cttttctatt tttataatat
      541 attgattttc aattatatac aatactttcc gaataacggg cactttaaca actatcacga
      601 ttcaccccag ctgggtcact gttgacctgc cattttaagc tgaaagcata aataagttga
      661 tgccgctggc cggtagggat attttgcatt tccaccctat tggattcacg cgtgcaacat
      721 ctatttgaaa ctactccccg taatttaacg cttggtcttc tcgcacttgt tgttgctgca
      781 ttcacctcat caagtccggt aaacgcggaa gctcccggag cacttccact cacgggaatc
      841 gatggtacgg atcgttactc agcaaccgtt tgcgcaggca gcagcatctg ctttgatgta
      901 ttcactcaac ccgataagga cgtgcagatc cgacgctggg tgggggatct tcccaacgcc
      961 ctgtttgagc tgttaaacgc gcaaggaaac agcggtcatt tatgctggac gccgggcgaa
     1021 caggatgcac gcgaggagcc ttatcgcttc accattttcg cagggactga aaacacagaa
     1081 gttcccgtgg agtacacctt actggtcccg cacatccaga tcagaagcgt gacgaaggag
     1141 atcagttgca aaggaatgga tgatggtcag atccttctgg aatcaatccc tgcagttgat
     1201 gagtatttct acacctggag cgacacccct ggacaaactc cggagcgaga tcatttgaaa
     1261 gccggcagct acttagtcgt tatcagcgac ccgaacggat gttccattga aagagaattc
     1321 gtgctggatg aaccagaagc gttgaccacg aaatacacgg tgattccctc cgattgccga
     1381 atagtcaacg gccgaatcaa actggaagta tcaggtggaa catggccata ccagtatgac
     1441 tggaacggct tgtccggatc cgaacctgaa ttgggtcccg tagcggaagg gtattatgga
     1501 ttagccttaa ccgatgcgaa tggatgcagt cttcgggacc gcattcatgt tccgtcagca
     1561 gcactgatgg tacaaaatgc caagatccag ccagcctcct gcccggaaca agcggacggt
     1621 agcgcgtcca ttacaatcaa tggtggcact gctccttatg agatcgagtg gtttccttac
     1681 ggcggatcag cacagcatgc agcagcgctc actgccggac tttataccgt tcaggttgcc
     1741 gatgccgccg gatgctccgc tacactggaa ttgtcgatcg aagcaagatc ccaaagtcca
     1801 aagctggaga tgccggctac cattgaattc tgtcaaggta gtcaggtcgc gctatcagcc
     1861 gggaatgcat ggcatacggt tgaatggagc gatggcagcc gacatagttc cttgtcggtc
     1921 aaccgggccg gaaaatattc cgtatccgtc accgatgatc atggttgtca acagcaatcg
     1981 acgatcgtgg caaccgcagt gaattgcaat gatcaacgtt cggcacgtcc gaccgactct
     2041 cccctgctac gggtttcgcc gaatcctgct ggtgatttga caacggtact gctggaccct
     2101 gaatatccgg aaggcacaac cctgttttta tcagacgcac ttggccgaca attgcaagtg
     2161 attacaccgc ggcgtgatgg cgctgagatt gaaatccctt tgcgcaacta cccggcagga
     2221 acttacctgc tgacgatcct tcagccttcc ggcagcgaga ccatccggtt gataaagtac
     2281 tgataacccg ccttagcatc gcttcaaaag tgaagtgggc gccaacagga aatacgctat
     2341 ttttgcggcg cattcctgcc cgtgtacgca ctctttcgta aagaactatc gagctttctg
     2401 ggatccctga ccgggtacct ggtgatgacc gttttcctgt tgtcggtcgg aatactcctg
     2461 tggatttttc cggatacttc cttcagcatt ccggataacc ggatcgccgc cctggacggc
     2521 ttgttcatcg tcgcgccctg gttgttcctg ttcctgatcc cggcgatcag catgcggatg
     2581 ttttccgaag agcagaagac cggaacactt gaactcctgc taacgcgtcc cctgagcgaa
     2641 tttcaattgg tcttcgcaaa gttcctcgcc gcgttcgtct tggtggtgtt gtcgttgatt
     2701 cctaccctga tctactactt cacggtctac cgtttggcgg ttccagccgg taatgttgac
     2761 acggcaggca tagcgggttc ttacatcgga ttattgttcc tgtcctccgg cttcgtggca
     2821 atcggcatct tttgctcggc gcaaacgaac aatcaggtcg tcgcgttctt gttcgcgctg
     2881 atcctctgtt acttcttcta cgccggattc gacgcattcg ccttgttagg aattcctgta
     2941 cggttgcaga acatcctgtc gcagttggga atcgccaatc actatgccgc gatgagtcga
     3001 ggcgtgatgg attcccgcga cttgctctat ttcgtcagcc tgaccacctg cttcctgcta
     3061 cttgcccgct tcacgctcga aaaaagaaaa tggtcatgag ttcgggcaag caacgcaagg
     3121 atagtctgct tcggctgatc gcctccctgg tgatcctggt ctcggtcaac atcatcggca
     3181 actttctctt tttccggatc gatctgaccg ctgaaaagcg atacagcatc agcgatgctt
     3241 cacgcgaact ggccggctcg ctgaatgatg tggtttattt caaggtctac ctcgatggcg
     3301 aactaccacc gggattccgg cggcttcgaa atgctacccg ggaattactc gatgaattca
     3361 ggatctacgc gggcggcaat atcgaatatg agttcgtcga cccctccgcc gaaccggagg
     3421 aaaagaaacg gctggaactc tacaagcaat tagccaggaa aggtctgctc ccaaccaatt
     3481 tagaggaacg tgaaaaaggc gggagtagtc agaagatcat tttccccggc gcgatcgctt
     3541 ccttcggtgg ggaggaaatt cccgttcaat tgctgaagag ccggcttggc agctcgccag
     3601 aagagatgtt gaacacgtcg gtcgaaaacc tggagtatga gatcgcggga gtgttacgtc
     3661 gcttcacccg caccggcgga gccccgcgta tcggcttcct gcgcggtcac ggcgaattgg
     3721 acaacaagca tatttccgac gcagcgcgcg cgttgaacga atattacaat gtagataccg
     3781 tcttcattgg tggtcgactc gacgccctga aggaccaccg gttgctgatc atcgccaagc
     3841 caacgctgtc gtttgacgag aaggacaagt tcgtcatcga tcagttcatc atgcgaggcg
     3901 gacgggt
//
LOCUS       DAOTON010000163         3882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18784_length_3882_cov_2.946172, whole genome shotgun sequence.
ACCESSION   DAOTON010000163 DAOTON010000000
VERSION     DAOTON010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18784_length_3882_cov_2.946172"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="P5292_04395"
     CDS             <1..830
                     /locus_tag="P5292_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS38399.1"
                     /translation="RNNFGCVTNDTITVTVIPPGIPNGGPDLVSCIGDSVILSGTQQN
                     AGGLIWSAAGDGTFQPSTTDPNVYYQPGTADTTNGVVQIFLTTTGACLNLQDTILVQL
                     LDLPTVSVGTDTVLTSGPGSGATVTLSGQALNAPTLQWTSTGSGTFVPSDTALNAVYE
                     PSDADFELDSVIVVLTATGGCLLVSDSLRIDFTPFVIPNVFTPYPASPGYNDYFEIKN
                     LPRNSLLKIWDRWGLLVFTSDDYRNNWDAANLNSDIYYYILETATGKYKGWIKVLRD"
     gene            908..2074
                     /locus_tag="P5292_04400"
     CDS             908..2074
                     /locus_tag="P5292_04400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HRS38400.1"
                     /translation="MKDQLLRFVDEGRIARWAVVITLAITVLLTAVQFHKKRSDQYTP
                     VNNYIIFKSSFHFLLTGEDLYALHPETHFDYYKYSPSFPLFMAPFYALPDLPGVLLWN
                     ILNSLLLVTGLLRLRFTDKRKEALALLFVLLETATSLQNSQSNGLIAGLAVWSLVFQR
                     DGKILPGTLLAALSVSIKVYGGLTILLFLFDRRWLKSLLAFGAWCMLIAALPLLVVSP
                     AQLQFLYESWGRLLSADHDASTGISLMGLLAPWLKFSSLKAVVTLLGLAALADYLWRS
                     LFAKQVDRNLRSLLILGHVLIWLVILNHKAESPTFVIAVTGAMLLYAGGPKDPNWRWM
                     LPLLLILTCLSPTDLFPKFIRDTWVVPYSLKALPCVVIYCYSFVQLHRLKKAAA"
     gene            2071..2457
                     /locus_tag="P5292_04405"
     CDS             2071..2457
                     /locus_tag="P5292_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRS38401.1"
                     /translation="MKKTWIAYLMFCLVGFSGMIVDFSITYLLKEFLLVNKFVANSIG
                     FTCAAISNYYLNSRWTFRESAKRNASSLFTFLFISTVGLALNNTLIYLMSPYFDFYLC
                     KLISTLLVSLFNFSANARITFRQRGA"
     gene            complement(2460..3434)
                     /locus_tag="P5292_04410"
     CDS             complement(2460..3434)
                     /locus_tag="P5292_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRS38402.1"
                     /translation="MHSPRYFQEKVERAISSLQLGEHPAELYEPIRYMLSLGGKRLRP
                     ALLLMANEAFGGREEDALGPALGIEVFHNFTLLHDDIMDKAPLRRSRETVHEKWNTDI
                     AILSGDAMFVRSCQLMAKCPDRYLRATLEDFHQTALLVCEGQQLDMNFEHQSLVRIED
                     YLEMISLKTAVLIGAALRMGARLGGASEEDAQHLYEFGRSLGVAFQLHDDILDAYGDP
                     EKFGKQVGGDILSNKKTYLLLTALDSADLHTLEELEFWIKRKEFNPAEKVQAVKTIFE
                     KLNVREKAEAELSRHFECALAELDCIPLPEEARKPLRELAEHLMVRES"
     gene            complement(3522..>3882)
                     /locus_tag="P5292_04415"
     CDS             complement(3522..>3882)
                     /locus_tag="P5292_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HRS38403.1"
                     /translation="AALKDRDITIYGAGKQTRTFCYIEDHLDATTNCFYNNLLVNDVA
                     NIGTDIEFTILELAQTIVRVTGSRSQIVHLPSLKEGDMTRRRPDSTKMRELLNRPFTP
                     LEDGLRAILANSHYVTS"
BASE COUNT          788 a         1107 c         1037 g          950 t
ORIGIN      
        1 tgcgcaataa cttcgggtgc gtgacgaacg acacgatcac ggtcacggtc attcctcccg
       61 gaatcccgaa tggcggcccg gatctggtga gttgcattgg cgacagcgtc atcctgagcg
      121 gaacgcaaca gaacgcgggc ggattgatct ggtcggcagc gggtgacgga accttccagc
      181 cttccacgac tgatccgaac gtttactacc agccgggtac tgcggacacg accaacggtg
      241 tcgtccagat cttcctcaca acgaccggcg cgtgcctgaa tcttcaggat acgattctcg
      301 tgcaactact cgatctccct acggtatcgg tcggcaccga tacggtcttg acctccggtc
      361 cgggcagcgg agctaccgtc accctgagcg ggcaagcact gaacgcgcct accctgcagt
      421 ggacctcgac cggaagcggc accttcgttc ccagcgatac agcgctgaac gcggtgtatg
      481 aaccttccga tgcagacttc gaactcgact cggtgatcgt cgttctgacc gcgaccggcg
      541 gctgcctgct ggtaagtgat tccctgcgta tcgactttac tccctttgtg atcccgaacg
      601 ttttcacgcc ttatcccgca tcaccggggt ataacgatta tttcgagatc aagaatctcc
      661 cccggaactc ccttttgaag atctgggatc gttgggggtt gttggtcttt acatccgacg
      721 attatcggaa caactgggac gccgccaacc tgaactcgga tatttattat tacatactcg
      781 agacggcgac cggcaagtac aagggctgga tcaaggtgtt gcgcgactag tccgccacta
      841 caaccagcgg aaaagtcttc tttcctccgg gtgggaagac ttttcttttt tactttgagg
      901 cgtccgcatg aaagaccagc tactacggtt tgttgatgaa ggtcgcatcg cccgctgggc
      961 ggtcgtgatc acgctggcaa tcacggtcct tttgaccgcg gtacagtttc ataagaagcg
     1021 atcggaccag tatacaccgg tcaacaacta catcattttc aaatcatcct ttcatttcct
     1081 gctgacgggc gaagacttgt acgccttgca tccggaaaca catttcgatt attacaagta
     1141 cagtccgagt ttccccttgt tcatggcgcc gttctacgca ctgccggacc tgccgggggt
     1201 attgctgtgg aatatcctga acagtctgtt gctggtaacc ggtctgctcc ggctgcggtt
     1261 taccgacaaa cggaaggaag ccctggcgct gctttttgtc ctgcttgaga ccgcgacctc
     1321 gctccagaac agtcagagca acggactgat tgccggactt gccgtgtggt ccctggtctt
     1381 tcaacgggat gggaagatcc tgcccggcac cttgttggcc gcgttatcgg ttagcatcaa
     1441 ggtttacggc ggactcacga tcctgctatt tctcttcgac cgtcgctggc tgaagtcgct
     1501 gctcgcattc ggcgcctggt gtatgctcat cgctgcacta cccctgctgg tggtcagtcc
     1561 ggctcagttg caatttctct acgaatcctg ggggcgtttg ctttcagcgg atcacgatgc
     1621 ctcaacgggc atctcgctca tgggtttgct tgctccctgg ctgaagttct cgtccttaaa
     1681 ggcagtggtc accttgctcg gcctggccgc gctggcggac tatctgtgga gaagcctgtt
     1741 tgccaaacag gtcgatcgga atctccgtag cctgctgata ctggggcatg tattgatctg
     1801 gctcgtcatt ctcaatcaca aggccgagtc gcccaccttc gttatcgcgg taacaggcgc
     1861 gatgttgctc tatgccggtg gaccgaagga tcccaactgg cggtggatgc tgcctttgct
     1921 gctgatcctg acctgcctct cgcccaccga tctgtttccg aagttcattc gtgacacctg
     1981 ggtggtgccg tacagtctga aggcacttcc ttgtgttgtg atctattgtt attcgttcgt
     2041 tcagttgcat aggctgaaga aggccgccgc atgaaaaaga cctggatcgc ttacctgatg
     2101 ttctgcctgg tcggctttag cggcatgatc gtggatttca gcataacgta cctgctgaaa
     2161 gaattcctgc tcgtcaataa attcgtcgcg aactcgatcg gcttcacctg cgctgcgatc
     2221 tccaattact atctgaacag ccggtggacc ttccgcgaat cggcgaaaag gaatgcctcc
     2281 tcgctcttca ccttcctttt catctctacg gtgggactgg cgctgaacaa tacgctgatc
     2341 tacctgatga gcccgtactt cgacttttac ctgtgtaaac tgatctcgac cttgttggtg
     2401 agccttttca atttcagcgc gaacgcgcgg atcaccttcc gtcaacgcgg agcctgaact
     2461 caactttcgc gcaccatcag gtgttccgcc aactcacgca gcggcttcct ggcttcctcc
     2521 ggaagcggaa tacaatccag ttccgccaag gcacattcaa agtgacggct caattcggct
     2581 tccgcctttt cccggacatt caatttttca aagatggtct ttaccgcctg caccttctct
     2641 gccgggttga actcctttcg tttgatccag aattccagtt cttcgagcgt gtgaaggtcc
     2701 gcggagtcga gggcggtcag gagcagatag gttttcttgt ttgacaggat gtcgccgccg
     2761 acctgcttgc cgaatttctc cgggtcaccg taggcgtcga ggatatcatc atgcaattga
     2821 aaagccacac caagcgatcg cccgaattcg tacagatgct gcgcgtcttc ttccgaagcg
     2881 ccacccagtc gcgcgcccat gcgcaacgcg gcgccgatca ggaccgcggt cttgaggctg
     2941 atcatctcga ggtaatcctc tattcgcacc agcgactggt gttcgaaatt catgtcgagc
     3001 tgctgaccct cacacaccag caaggcagtc tggtggaaat cctccagtgt cgcccttaaa
     3061 taccggtcgg ggcatttagc catgagctgg catgaacgga cgaacatcgc gtcgcccgag
     3121 aggatcgcga tatcggtatt ccatttctcg tgcaccgtct ctctggacct gcgcaaaggc
     3181 gccttgtcca tgatatcgtc gtgcaacaaa gtgaaattgt gaaacacctc gatgccgaga
     3241 gcgggcccca gcgcatcttc ttcccggcct ccgaaggctt cattggccat cagcaacaaa
     3301 gcaggtcgca ggcgtttccc acccagcgag agcatgtacc gtatcggctc gtacagttcg
     3361 gcgggatgtt cgcccagttg cagactgctg attgcgcgtt ctactttttc ctgaaaataa
     3421 cggggcgaat gcatgaaaca gttaacagcc gtgatcggta tttgttcgga ataaaatcaa
     3481 aaacccggcg aatgaaaacg cagggtgacg tcgaccccag atcaggaggt cacgtagtgc
     3541 gagttcgcga ggatagcgcg caatccgtct tccagcgggg taaacggacg attcaacagt
     3601 tcgcgcatct tggtgctgtc gggacgacga cgggtcatgt cgccctcctt cagtgaaggc
     3661 aggtggacga tctgactacg cgagccggtc acgcggacga ttgtttgcgc gagctccaga
     3721 atcgtgaact cgatgtcggt accgatgttc gcgacatcat tcacgaggag gttattataa
     3781 aagcaattgg tcgtggcatc gagatggtct tcgatataac agaacgttct cgtctgtttg
     3841 ccggcaccat agatggtgat gtcccggtcc ttgagggcgg cg
//
LOCUS       DAOTON010000164         3881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18793_length_3881_cov_3.142185, whole genome shotgun sequence.
ACCESSION   DAOTON010000164 DAOTON010000000
VERSION     DAOTON010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18793_length_3881_cov_3.142185"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="P5292_04420"
     CDS             complement(<1..1173)
                     /locus_tag="P5292_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS38404.1"
                     /translation="MKILFTHGYFLREDPREQQIMKPYPPLGILYLSAWLEQNGFINE
                     VFDSTFSDFETLCRKLEQEPPQVLAVYTNLMTKLNVLRLIRHVRSTEALSGTRIVLGG
                     PEVRNHAENFLRHGADLIVVGEGEETMLDIVRRLQERGAGIRNFELATGIDGCITLDD
                     RGELSQSPERRLLRDLDELPAPARHQIQLTRYLDAWRSRHGYSAISVSTMRGCPYTCK
                     WCSRAVYGQTYRRRSPEKVVDELAQLKQRYDFDTIWFVDDVFTISHRWLEGFRDALVN
                     RGLDLRFECITRADRMNETVIRLLKESGCFRVWIGAESGSQSVIDAMDRRVDVQQVRT
                     MIRATRAAGMEAGTFLMLGYPGETEADIEETIRHLKDSRPDHYTITLAYPIKGTPLY"
     gene            complement(1170..2006)
                     /locus_tag="P5292_04425"
     CDS             complement(1170..2006)
                     /locus_tag="P5292_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRS38405.1"
                     /translation="MNPTVPFDAVAQAYDQQFTRSATGRLQRERVHRFIETMPLLKKP
                     ARVLELNCGTGEDALWLARKGHQVTATDNSAGMLDAAREKFRNADPLHGSVRFLHYDL
                     NGPPAAGWQSSYDLIFSNFGGLNCLDASSIQRLSGKLVQYLRPGGKLVLVIMGRRCLS
                     EQLYFLSKGHPQKAFRRKSKQPVTVHLADGHEQLTWYYRPGELDHLLNPHFRRSHLEP
                     VGFFLPPSYLDHRVARHPRLLRLLKRLEDSLQGWSLLADRADHYLAEFTSLRQDNPVK
                     TA"
     gene            complement(2003..3466)
                     /locus_tag="P5292_04430"
     CDS             complement(2003..3466)
                     /locus_tag="P5292_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS38406.1"
                     /translation="MKRLLFTHSYFLRFDPKQWKAQQAFPPLGTLYAAGVARESGHVV
                     SLFDSQFERSPESLLPRLERERPEVLVVWDDGFNYLTKMCLTNMREAALHMIRSASER
                     GITVLVSSSDASDHPELYLAAGAKYVLAGEGEQTLKELLDQDIPPFAPEIPGLIWQEA
                     GVLKRSAAREVLRDLDRLPRPAWDLVDMQSYRERWLKSAGRFSLNISTTRGCPFKCNW
                     CAKPIYGNRYNIHGPERVVQDLIYLKEQYQPDHLWITDDIFGLKPGWVEAFAGLIEKA
                     GIRIPFKIQSRVDLLLEENTVAALARAGCEEVWVGAESGAQHILDAMDKGTTVTQIRE
                     ATRLLKSHGIRPAFFLQFGYPGETLEDIRATIRMVEELEPADIGISVSYPLPGTVFHE
                     RVRSDMRSKQNWTDSDELAVMFRSTYPGSFYKELQRYVHRRFRARQALAALRGWRAER
                     SLSPKALRRIAALPVYYTRALLHRRKLEAQLEQKLLA"
     gene            complement(3463..>3881)
                     /locus_tag="P5292_04435"
     CDS             complement(3463..>3881)
                     /locus_tag="P5292_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38407.1"
                     /translation="HNAFTATEIAFVIPTYNYTLYRKFLHANDWYRHHYPNFPQRDQR
                     YIVQERSPRIKRSVEFLLSGQPGTWLDRWCFVKTLGFWKRKFRHFDPGTFDRRLRSRR
                     NVSKHNPLGYQDRVLRLLDERLRSFEQRFELAPFSL"
BASE COUNT          816 a         1098 c         1124 g          843 t
ORIGIN      
        1 gtacaagggg gtgcctttga tcgggtaggc taaggtgatg gtataatgat cgggacgtga
       61 atccttcaga tgacggatcg tttcttctat atcggcttcc gtctctcccg gatatcccag
      121 catcaggaaa gtgccggcct ccatacccgc ggctcgcgtt gcccggatca tggttcgcac
      181 ctgttgcacg tcgacgcgcc ggtccatcgc gtcaatcacc gactgtgagc cgctttccgc
      241 cccgatccag acgcggaagc aaccgctttc cttcaacaag cggatgacgg tttcattcat
      301 ccggtcggcc cgggtgatgc attcgaaccg gaggtcaagc ccgcggttca ccaacgcgtc
      361 cctaaatcct tccagccagc gatgactgat cgtgaacacg tcgtccacaa accagatggt
      421 gtcgaagtcg taacgctgtt tcaattgtgc cagttcatcc acgacttttt cgggactgcg
      481 ccttcggtag gtctggccat acacggcccg gctgcaccac ttgcaggtat acggacatcc
      541 gcgcatggta ctcaccgaga tggcgctgta gccgtgacgg gatcgccagg catccaggta
      601 gcgcgtcaat tggatctgat gtcgcgcggg agccggcagt tcgtccaggt cgcggaggag
      661 ccggcgctcc ggcgattggc tcagttcgcc gcgatcatca agcgtgatac agccgtcgat
      721 tcccgttgcg agttcgaagt tcctgatacc tgctccgcgt tcctgtaaac gtcgtacaat
      781 gtccagcatc gtttcttccc cctcgccaac cacgatcagg tcggcgccgt gacggagaaa
      841 attctccgcg tgattccgaa cctccggtcc gcccagcacg atccgcgtac ccgacaaggc
      901 ttcggttgaa cgtacatgac ggatcaggcg caggacgttc aatttcgtca tcaggttcgt
      961 gtacacggcc agcacctgcg gaggttcttg ctcgagtttg cggcagagcg tttcgaaatc
     1021 cgaaaaggtc gagtcgaaga cttcgttgat gaagccgttc tgttccagcc aggccgacaa
     1081 atacaagatg cccagtggcg gatagggttt catgatctgc tgttcacgcg gatcttccct
     1141 caggaaatag ccgtgggtaa agaggatttt caagcggtct tgaccggatt atcctgacgc
     1201 agcgaagtga attcagccag gtagtgatcg gcccggtcgg cgagcagcga ccagccttga
     1261 agggagtctt ccaaccgttt gagcaggcgc aacaaccggg gatgccgcgc cacccgatga
     1321 tcgagatagc tcggcggcag gaaaaagccg acgggttcca aatgagaacg ccgaaaatgc
     1381 gggttgagga gatggtccaa ctcgcccggc cggtagtacc atgtcaattg ttcatggcca
     1441 tcggccagat gcacggtaac cggttgtttg ctcttcctgc gaaaggcttt ttgcggatga
     1501 cctttcgata gaaaatacaa ttgctcactc agacaacggc gtcccatgat caccagcacg
     1561 agcttgcctc ccggtcgcag gtactggacc agctttccgg acagccgttg gatcgatgac
     1621 gcgtccagac agttgagtcc gccgaagttc gagaaaatca aatcgtagga ggattgccaa
     1681 cccgctgccg gggggccgtt caaatcataa tgaagaaagc gaacgctacc gtgcagtgga
     1741 tcggcattcc ggaatttctc tcgcgcggca tccagcatgc ctgctgagtt atcggtggcg
     1801 gtgacctgat gtcctttccg ggccagccat agcgcgtctt ctccggtgcc gcaattcaac
     1861 tcgagcacgc gtgcgggctt cttcaacagc ggcatcgttt cgataaaacg atggacgcgt
     1921 tcacgctgga gtcggccggt ggcactccgg gtgaactgtt ggtcgtacgc ctgcgcgacc
     1981 gcatcgaacg gcacggtggg attcatgcca ggagtttttg ttccaactgc gcctccagct
     2041 ttcgccggtg cagcagcgca cgggtatagt aaaccggtaa tgccgcgatg cgacgcaatg
     2101 ctttcgggga gagggagcgc tccgccctcc atccgcgcaa ggctgctaat gcctgtcggg
     2161 cgcggaaacg ccggtgaaca tagcgttgca attctttata gaaggagccg ggataggtgc
     2221 tgcgaaacat caccgccagt tcatcggagt cggtccagtt ctgtttcgat cgcatgtccg
     2281 atcgcacacg ttcatggaat accgttccgg gcaggggata actcaccgaa atgcctatgt
     2341 cagcaggctc cagttcttct accatccgaa tggttgcacg gatgtcttcg agggtttccc
     2401 ccggataacc gaattggaga aagaaggcgg gtcggatgcc gtgtgacttc aacaatcgcg
     2461 tcgcttcccg gatctgtgtc accgtggtgc ctttgtccat cgcgtcgaga atgtgttgcg
     2521 cgccgctttc tgcgcctacc cagacttcct cgcaaccggc acgtgccaaa gcggcgacgg
     2581 tattctcctc cagcaacagg tcaacgcgcg attgtatctt gaatggaatg cggatgcccg
     2641 ctttttcgat cagcccggcg aatgcttcca cccagccggg ctttaacccg aagatgtcgt
     2701 cggtgatcca aagatgatcg ggttgatact gttctttcag gtagatcagg tcctgcacca
     2761 cccgctccgg accgtgtatg ttgtagcgat tcccgtagat cggcttcgcg caccagttgc
     2821 atttgaacgg acatccacgg gtcgtactga tgttgagtga aaacctgccg gccgatttca
     2881 gccagcgttc acggtacgat tgcatgtcca cgaggtccca ggccggccgg ggcagccggt
     2941 ctaagtcgcg aaggacttca cgggctgccg aacgtttcaa aacgcccgct tcctgccaga
     3001 tcagtccggg aatctccggg gcaaacgggg gaatatcttg atccaacaat tccttcaagg
     3061 tctgttcgcc ttctccggcc agtacatatt ttgctccggc agcgaggtac aactccggat
     3121 gatcactggc atcgctggac gataccagca ccgtgatacc acgctccgag gccgaacgga
     3181 tcatgtgcaa cgcggcctcc cgcatgttgg tgaggcacat tttggtcagg tagttgaagc
     3241 cgtcgtccca tacgaccagt acctccggac gctcacgctc cagtcgcggg agcaacgatt
     3301 ccggtgatcg ttcaaactgc gaatcaaaca gggatacgac atggcccgat tcgcgcgcaa
     3361 caccggcagc atagagggtg cccagcggcg gaaaggcttg ctgcgctttc cactgtttgg
     3421 ggtcgaagcg taaaaagtaa ctgtgcgtga acagcagcct tttcatagcg agaagggcgc
     3481 cagttcgaac cgttgctcga atgaacgtaa gcgttcgtcg agcagacgga ggacccggtc
     3541 ctgataaccc aacggattat gcttggacac gttgcggcgg cttcgtagtc gtcggtcgaa
     3601 cgttcccgga tcgaaatgcc ggaattttcg tttccagaaa ccaagcgtct tgacaaaaca
     3661 ccaccggtcc agccaggtgc cgggttgacc actcaggagg aattcgacgg atcgtttaat
     3721 gcggggcgaa cgttcctgca cgatgtaacg ctggtcacgt tgcgggaaat tcggatagtg
     3781 gtggcggtac cagtcgttcg catgaaggaa cttacggtac agggtgtagt tataagtggg
     3841 aataacgaag gcgatctcgg ttgccgtaaa ggcgttgtgg t
//
LOCUS       DAOTON010000165         3881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18794_length_3881_cov_3.139833, whole genome shotgun sequence.
ACCESSION   DAOTON010000165 DAOTON010000000
VERSION     DAOTON010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18794_length_3881_cov_3.139833"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="P5292_04440"
     CDS             complement(<1..310)
                     /locus_tag="P5292_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38408.1"
                     /translation="MSRLCRILLFFLSLAIAADGTHIVGGEMSYLSLGGNQYRVTLTV
                     YRDCYNGQAGFDNPAAIGIFDSNGDLVASLDATITNSGAVANTINSPCLVPPTNVCYE
                     Y"
     gene            complement(394..1332)
                     /locus_tag="P5292_04445"
     CDS             complement(394..1332)
                     /locus_tag="P5292_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRS38409.1"
                     /translation="MSTQQFRPAIAIHGGAGTILRSVMRPEQEKEYHHALKAALESGY
                     NILQAGGSSLDAVVAAVESLEDCPLFNAGRGAVFTNEGKHELDAAVMIGNSRAAGAVA
                     SVSGVRNPVRLARAVMEHSEHVLLAGAGAEQFAKSRAIAFEPEDYFFTRQRYDQWQEA
                     MREGVVQLDHRDDKKFGTVGAVALDNAGNVAAATSTGGMTNKRFGRIGDSPLIGAGTW
                     ADNTTCAVSCTGHGEYFIRAVAAYDVACLMEYKGMTLRQACEEVVMRKLKPAGGEGGL
                     IALDRTGVPVLIFNSEGMYRGWQTMPGAPNTAIYGD"
     gene            1653..3641
                     /locus_tag="P5292_04450"
     CDS             1653..3641
                     /locus_tag="P5292_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38410.1"
                     /translation="MMKKLYTLALLTCCYLLTGSAFAQLPNLRGIYVNQFSSILGNTA
                     KEDSLFKYAQDSSFNYLAFYDLHSFDLTNATKANQLASFLRRGREQFGIPYMGAVGES
                     WNFFVNKMKPYQNSRSNVNERFNVFNVEFEFWTSSSVNSGGYYCTQYLQPAGCNCDTA
                     GAFKFYKDLLRKVDSLATSLNVLSETYVGWFNAGQAKELQPLVDRILVHAYRLDPSSV
                     FGYSKTRLGYLAGNNLPVNVVPIFSSEPDFMGSGWIQSHAQIEAYNKYLTDYNADNSA
                     WKAHINLQGYQWFCYGYMPKPVPGTGGATTTFNPIITASGSTSFCIGGGVGLAASTGD
                     SYLWSNGATTPSISVSTAGTYTCRVTKAGVSATTPAVTVTVFNAPSVVMTVGAPLSAS
                     VPLTANAAAGSGTISSYQWLLNGSTIGNAIGAGYDATASGNYSVRVTNSNGCSATSAS
                     QSVSVPVTVACQLTAPSGLTSTILSSTSVKVTWNAVSQCDTIVVRYKREGTNTYNYIR
                     MPYSNQTSTTINNLTAGARYSWRVKTVCGTTSSSYSSKKYFTPMNASELPHNELALRR
                     LDDGTELESRELGIDLSVYPNPADYQVKLYFLAEEEANAQVDIFDLNGRIVLSQQVQS
                     VEGDNFVMLETSQLLPGLYVVSVRTTDTFYSARLSVNH"
BASE COUNT          924 a         1130 c          973 g          854 t
ORIGIN      
        1 cgtattcgta gcaaacattg gttggaggga ccaggcaggg gctgttgatc gtgttggcga
       61 cagcgccgga attggtgatg gtggcatcca gtgaagcgac cagatcgcca ttactgtcaa
      121 agatgccaat ggctgccgga ttatcgaatc cggcttgtcc gttgtaacaa tcccggtaaa
      181 ccgtgagggt gacccgatat tgattgccgc ccagggagag ataggacatt tctccaccga
      241 caatgtgtgt tccgtctgcc gctattgcca gcgacaaaaa gaacaacagt atcctgcaga
      301 gacgggacat gcgaaagacg ggacttggaa agccactaaa gatacgacac gcgagcaggc
      361 ggggaaagac cgaccgatca gtaaattgtc atatcaatcg ccgtaaatcg cggtatttgg
      421 ggcaccgggc attgtttgcc agccgcggta cataccttct gaattaaaga tcagaaccgg
      481 aacaccggta cggtccaggg caatcagccc cccttctcca ccggccggtt tcaatttacg
      541 catgaccacc tcttcgcagg cttgccggag cgtcatgcct ttgtattcca tgagacaagc
      601 gacatcgtac gcggcaacgg cccggatgaa atattccccg tggccggtac aggaaaccgc
      661 acaggtcgtg ttatcggccc aggtgccggc gcctatgagg gggctgtcgc cgattctgcc
      721 aaagcgtttg ttggtcattc caccggtgga agtcgccgcg gctacattgc cagcgttatc
      781 gagcgccaca gcaccaacgg ttccgaattt cttatcgtcc cggtgatcca actggacaac
      841 accttcgcgc atggcttcct gccattggtc gtaccgttgc cgggtgaaaa agtagtcttc
      901 aggttcaaac gcaatcgcgc ggcttttcgc gaattgctcc gcacctgcgc cggccagcaa
      961 cacatgttcc gaatgctcca tgacagcacg cgcgagtcgt accggattcc gaacaccgct
     1021 cacagacgca accgctccgg ccgcgcgtga attgccgatc atcaccgcgg catccagttc
     1081 gtgtttaccc tcgttggtga acacggcccc tcttccggca ttgaagagcg gacagtcttc
     1141 caaagattcg acggcagcga caaccgcgtc aagagaagat ccgccggcct gcaggatgtt
     1201 gtagccggat tccaacgcag ccttcaaagc atggtggtat tctttctcct gttccggacg
     1261 catgacgctt ctcagaatgg tgcctgctcc tccatgaatc gcgatagccg gacgaaattg
     1321 ctgggtcgac atagtcccgc aaagaaacga agctgaaatc gaactgggat cagattacag
     1381 gaaaatgaag aaatacccgt tccgggaaca ccatttcagc cgaaaatagg aacgagcaca
     1441 ggtgctgtac ccccttgcct tggacttgga attcatgatt cctcccactt agttctttga
     1501 attctttaaa aaaacgaggc ttcaccctca atcctgaata tttgatcgga aagccgtcac
     1561 aaccttacat tttcaattga gcccgtttct ctcgcaaatc ggggtatatc acgcggggta
     1621 ctttgcaatc gttgtttatc tgcgaagaca ccatgatgaa aaaactctac accctcgcgc
     1681 tgctgacctg ctgctacctg ctgaccggtt ccgctttcgc tcagctcccg aaccttcggg
     1741 ggatctacgt gaatcaattc agctccatcc tcggcaatac cgccaaggaa gacagccttt
     1801 tcaagtacgc gcaagacagc tcgttcaact acctggcgtt ttacgacctg cattcctttg
     1861 acctgacgaa tgcgaccaaa gccaatcaac tggcttcgtt cctgcgtcgt ggtcgtgaac
     1921 aattcggcat tccgtacatg ggtgccgtgg gcgaatcctg gaacttcttc gtgaacaaaa
     1981 tgaagccgta ccagaacagc cggtcgaacg tgaacgaacg tttcaatgtc ttcaacgtgg
     2041 aattcgaatt ctggaccagt tcatccgtga attcgggcgg atactattgt acccagtatc
     2101 tgcaacctgc aggatgcaac tgcgataccg ccggggcttt taaattctac aaagacctcc
     2161 tccgtaaagt agactccctg gccacttcgc tgaacgtcct ttccgaaacc tatgtaggct
     2221 ggttcaatgc aggacaggca aaagaactcc aaccgctggt tgaccgtatc ctggtgcatg
     2281 cctaccggct tgatccttcc tccgtctttg gttacagcaa gacgcgactg ggttatctcg
     2341 caggaaacaa cctcccggtg aatgtcgtac cgatcttctc ttccgaaccc gatttcatgg
     2401 gcagtggctg gatccagtcg catgcgcaga tcgaagctta caacaaatac ctgactgatt
     2461 acaacgcaga caactccgcc tggaaagcgc acatcaacct tcagggatac caatggttct
     2521 gctacggcta tatgccgaaa cccgtccccg gtaccggtgg tgccaccacg accttcaatc
     2581 cgatcattac cgccagtggc agtaccagct tctgcatcgg tggcggcgta ggtttggctg
     2641 cgagcacggg cgacagctac ctctggtcca acggcgcaac cacgccatcc atttcggtat
     2701 ccacggccgg aacctatacc tgtcgggtta ccaaagcagg tgtctccgcg accactccgg
     2761 cggtgaccgt tacagtcttc aatgccccga gtgtcgtgat gacggtcggc gcgccgcttt
     2821 cggccagtgt accgctgacc gcgaacgcgg ctgcgggaag cggaacgatc agcagctacc
     2881 agtggttgct caatggaagc acgatcggga acgcgatcgg cgcgggctat gacgcgacgg
     2941 cttccggcaa ctacagtgtg cgggtgacca acagcaatgg ttgctccgca acatcggcaa
     3001 gccagtcggt ctccgtaccc gtaaccgttg cctgtcagtt gaccgctccc agcggcctca
     3061 cctctaccat cctctcctct accagcgtga aggtcacctg gaacgccgtt tcgcaatgtg
     3121 ataccatcgt ggtccgctac aagcgggaag gcaccaatac gtacaactat atccggatgc
     3181 cgtacagcaa ccagacctcg accaccatca acaacctgac ggccggcgcg cgttattcct
     3241 ggcgcgtaaa aacggtttgc ggcaccactt cgagttcgta ttcgagcaag aagtacttca
     3301 cgccgatgaa cgcatccgaa ctcccgcaca acgaactggc gcttcgccgg ctcgacgatg
     3361 gcaccgaact cgagtcgcgt gaactgggca tcgatctctc cgtttatccg aatcctgccg
     3421 actaccaggt gaaactgtac ttccttgccg aggaagaggc aaacgcgcaa gtcgacatct
     3481 ttgacctcaa cggaagaatc gtcctgagtc agcaagtcca gtcggtcgaa ggcgacaact
     3541 tcgtgatgct ggaaaccagc caactgctgc ccggactgta tgtagtgagc gtccgcacca
     3601 ccgacacatt ctactcggcc cgcctgagcg tgaaccattg atcgccaaac gaatccatcc
     3661 aaaaagcccg ggaaatcccg ggctttttgc ttttccccaa attttaacat ttcgagttat
     3721 caacacacca agtggtcgag tttcattttt cttccaaatt gttgtgcttt tcaaggagaa
     3781 aaaagccttc aaatttttaa aaattggagg ttaggtgaac cgggagccaa catcgttaat
     3841 atccattttt gcctcagaat cacgaggata aacgctgatt a
//
LOCUS       DAOTON010000166         3863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18914_length_3863_cov_2.980567, whole genome shotgun sequence.
ACCESSION   DAOTON010000166 DAOTON010000000
VERSION     DAOTON010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18914_length_3863_cov_2.980567"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /gene="nth"
                     /locus_tag="P5292_04455"
     CDS             <1..482
                     /gene="nth"
                     /locus_tag="P5292_04455"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRS38411.1"
                     /translation="ISGYAWGLGYFGGLLSTVGVAGMARMLVERFKGKVPSSVEELVQ
                     LPGVGRKTANVIASVVYNLPAIAVDTHVFRVSARLGLTVRAKNPLQTERQLVKFIPEN
                     KLAIAHHWLILHGRYVCIARKPACEGCGLTRYCKYFESTFLKKKDIRPRKVPPRSSR"
     gene            complement(445..1230)
                     /locus_tag="P5292_04460"
     CDS             complement(445..1230)
                     /locus_tag="P5292_04460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS38412.1"
                     /translation="MIQSYAIITGASKGIGRAIALELARRGYSLLLIARSATLLEETA
                     ALARKAGAAEVRPMALDLTKAESVTAVLQEVQRNNLPVNVLVNNAGYGAWGRFDTLSL
                     EEQDAMMRINMHVPVELTHGLLPLLKSRQPAYILNVASTAAYQAVATLALYAASKAFI
                     LQFSRALRIELKKSGVSVTCLSPGPTETNFMSQAGMHHPKMIQRASKFNMTPEAVAKF
                     AVKGLFAGKNEIIPGVTNSVSALLTRFVPKALTEKIAAGLYEV"
     gene            complement(1303..2112)
                     /locus_tag="P5292_04465"
     CDS             complement(1303..2112)
                     /locus_tag="P5292_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRS38413.1"
                     /translation="MNNASYPKFKLGDQLTPEQIAFFNKNGFIHFSGVASPQEVADII
                     RSTEDIQSKWIREGVKKINGVPIKFGIDENNKTIVHRFCFTSQYCEPVHAFVNSQRVK
                     ALKVLMPDGARIAENEKDGVVFNHYVNTDNSNFRQMGWHTDSMRDIFYGKKIMPMLNV
                     GLYLDDSSSDKGGLRIIPGTHKQNLFSMLFKKAYFMNNEEDKNEMLVDAKAGDMVVHD
                     GRIWHRVAMSPHKGAASRRRVMYIPVILGKYQPKDDGSPTPLYHHFQKLVR"
     gene            complement(2319..3644)
                     /locus_tag="P5292_04470"
     CDS             complement(2319..3644)
                     /locus_tag="P5292_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRS38414.1"
                     /translation="MKLQVKLALYNAISKALIILAIGLVIPLIIERVVLRHMDKRLLA
                     RRDKMMMMIGKGGLNEITLDQDCSFDSYSVFKEEYVSISPLTAYPKDFGKPSFRDTTQ
                     LIENEAVKHRVLRQAFLYDNQLYQLQIGEGMSTIDQLNHTIRNFTVDLMLVVVLISIF
                     LDIGFVQLILRPFNRIINEKLRQMNHPTGYRPVSIKTNTYEFNYLDRSIDEMMQKVQE
                     SFRTEREFITNVSHELLTPVSILRNRVENILNDPNVPEEIQAKMVESQKTLSRLTRVV
                     KALLYISKIDNDQYLRNEEADLQELMTDVVAELEDRVHEKDLRLVQEWVDPFVVRNCN
                     RSLLHTLLLNLVSNAIKYNKPGGRISIRGWSGEQHYVLTIQDSGVGIREEQLPFIFDR
                     FKRFRPEDGMSYGLGLPIVRSIAQIHEIDVDVQSEPEVGTTFTLRFPLV"
     gene            complement(3670..>3863)
                     /locus_tag="P5292_04475"
     CDS             complement(3670..>3863)
                     /locus_tag="P5292_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRS38415.1"
                     /translation="LHKNRVITRVQLTEHIWGDILEEDYESNYIDVHIKNLRKKLSAH
                     ANAEFIETVRGIGYRLNMP"
BASE COUNT          833 a         1001 c         1087 g          942 t
ORIGIN      
        1 agatttccgg ctatgcttgg ggactgggct atttcggcgg actgctgtcc acggttggcg
       61 tggccggcat ggcgcgcatg ctggtggagc gattcaaggg caaggtgcct tcctctgtag
      121 aggaactcgt gcaactcccc ggagtaggac ggaagaccgc caacgtgatc gcgtcggtcg
      181 tgtacaactt gccggcgatc gcggtggata cacacgtgtt tcgtgtttcg gcaaggctgg
      241 gcctgacggt gcgtgcaaag aaccccttac agacggaacg acaactggta aaattcatcc
      301 cagaaaacaa gctggccatc gcgcaccact ggctgatcct gcacggacgc tatgtctgca
      361 tcgcacgcaa acccgcctgc gaaggttgcg gactcacccg ctactgcaag tatttcgaat
      421 cgaccttcct gaagaaaaag gacattagac ctcgtaaagt cccgccgcga tcttctcggt
      481 gagcgccttc ggaacaaaac gcgtgagcag agccgacacc gaattggtga cgcccggaat
      541 gatctcgttc tttccggcaa acaacccttt cacggcaaac ttcgccacgg cttcgggcgt
      601 catgttgaac ttgcttgcgc gttggatcat cttcggatga tgcatcccgg cctgcgacat
      661 gaaatttgtt tcagtcggtc ccggactcaa acaggtcacc gaaacacctg acttcttcaa
      721 ctcaatccgc aaggcccggg agaactgcag gatgaatgcc ttggaagctg cataaagggc
      781 cagggtcgcg accgcctggt aggcagcggt gctggcgacg ttcaggatgt aggccggttg
      841 ccgggatttc aacaagggca gcaatccatg tgtgagctcc accggtacgt gcatgttgat
      901 gcgcatcatc gcgtcctgtt cttccaggct gagtgtgtcg aagcggcccc aggcgccgta
      961 tccggcattg ttgaccagca cgttcaccgg cagattgttc cgttgcactt cctgcaaaac
     1021 ggcggtaacc gattcagcct tggtgaggtc cagcgccatc ggacgtacct ccgctgctcc
     1081 ggcttttcgg gcaagtgccg cggtttcttc cagcagtgtc gcggagcgag cgatcagcag
     1141 caatgaatag ccgcggcggg caagttccag ggcgatagcg cgaccgattc ccttactggc
     1201 gccggtgatg attgcgtagg attgaatcat gttgaaggat aaaaagaaac cggaaccaca
     1261 tgctggctcc ggtcttctgt tctttatcga agaatcgctt ccttagcgga ccagcttttg
     1321 gaagtgatga tagagcggag tggggctgcc gtcgtctttc ggttggtact ttccaagaat
     1381 gaccggaatg tacatgacac ggcgacggga agccgcgcct ttgtgcggcg acatcgcgac
     1441 gcggtgccag atgcgaccat cgtgcacgac catgtcgccg gcttttgcgt caacgagcat
     1501 ctcgttcttg tcttcttcgt tgttcatgaa gtaggccttc ttgaacaaca tggagaaaag
     1561 gttctgcttg tgtgtgccgg ggatgatgcg caatccgcct ttgtcggaag atgagtcgtc
     1621 gaggtaaagg cctacgttca acatgggcat gatcttcttg ccgtagaaga tatcgcgcat
     1681 gctgtcggta tgccagccca tctggcggaa attgctgttg tcggtattga cgtaatgatt
     1741 gaataccacg ccgtcctttt cattctcggc gatacgagcg ccatcaggca tcagcacctt
     1801 gagcgccttc acccgctgac tgttcacaaa cgcgtggaca ggttcacagt actgggaagt
     1861 gaagcaaaag cgatgaacga tggtcttgtt attctcgtct attccgaatt taatcggaac
     1921 gccgttgatc ttcttcacac cttcccggat ccatttcgac tggatgtcct cggtggaccg
     1981 gatgatatcg gccacttcct gcggactggc tacgccggag aaatggataa agccgttttt
     2041 gttaaagaat gcgatctgct ccggtgtgag ttggtcaccc agcttgaact tcggatagga
     2101 tgcgttattc atcagctttt taggtcagag tattggaccg gttgtagacc ggcaaaggaa
     2161 tacacgcaga aataacttcc tgtgaacccc ctatacgggc aaaaataggc cttcgaatag
     2221 ttacttccaa aactgtctat tcttgatttt caacaattta acgctctatt aaggattctt
     2281 cggtggctgg aaaggaaggt gcatccggaa ttagagaatc agaccagggg aaatcgaagg
     2341 gtaaaggtgg tgcccacctc gggttcactc tgaacatcta cgtcgatttc gtggatctgg
     2401 gcgatactgc gaacgatcgg caaccccagg ccatagctca tgccgtcttc cggacggaag
     2461 cgcttgaatc ggtcgaagat gaacgggagt tgctcctccc ggatcccgac tccgctatcc
     2521 tggatggtca gtacgtaatg ttgctcaccc gaccagccac ggatcgatat tctgcctccg
     2581 ggtttattgt atttgatggc gttggatacc aggttgagca acaaggtatg caacagggat
     2641 cggttgcagt ttcgcacgac aaacggatcg acccattcct gtaccagccg caggtctttt
     2701 tcgtgcacgc ggtcttccag ttcggcgacg acgtcggtca tcaattcctg caggtcggct
     2761 tcttcgtttc ggagatattg gtcgttatcg atcttcgaga tgtacaacag tgccttgacc
     2821 acccgcgtca accgcgacaa ggtcttctgg gattcgacca tcttggcctg tatctcctcg
     2881 gggacgttgg gatcgttgag gatgttctcg acccggttac gcaggatgga taccggcgtc
     2941 agcaattcgt gcgatacatt ggtgatgaat tcccgctctg tccggaacga ctcctgtact
     3001 ttctgcatca tctcgtcgat cgaacggtcg aggtagttga attcgtaggt gttggtcttg
     3061 atgctcaccg gtcggtaacc ggtcgggtga ttcatctgtc gcaacttctc gttgatgatg
     3121 cggttgaacg gccgcaggat caattggaca aaaccgatat cgagaaagat ggagatcaga
     3181 acgaccacca gcatcaaatc gaccgtgaaa ttgcgaatgg tgtggttgag ctgatcgatc
     3241 gtgctcatgc cttccccgat ctgcagttgg tacagctggt tgtcgtacag gaacgcctga
     3301 cgcagtacgc ggtgcttgac cgcttcgttt tcgatcaact gggtggtgtc ccgaaaactc
     3361 ggtttaccga agtccttcgg ataagcggtg agcggcgaaa ttgagacgta ttcttctttg
     3421 aaaaccgagt aggagtcgaa gctgcaatcc tgatcgaggg tgatctcgtt caagccgccc
     3481 tttccgatca tcatcatcat cttgtcgcgg cgagccaaca gacgtttgtc catatgacgt
     3541 agcacgacac gttcgatgat gagcgggatc accaggccga tggccaaaat gatcagcgcc
     3601 ttcgaaatgg cgttgtagag tgcgagcttg acctggagtt tcacggtagg atccttgtcc
     3661 ggccgcagct caaggcatgt tcaaccggta accgatgccg cggacggttt cgatgaactc
     3721 ggcattggcg tgcgcggata atttcttgcg caggttcttg atgtggacgt cgatataatt
     3781 cgattcgtag tcttcttcca ggatgtcgcc ccagatatgt tccgtcaact gcacccgggt
     3841 gatgacccgg ttcttgtgca ggg
//
LOCUS       DAOTON010000167         3862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18917_length_3862_cov_3.683740, whole genome shotgun sequence.
ACCESSION   DAOTON010000167 DAOTON010000000
VERSION     DAOTON010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18917_length_3862_cov_3.683740"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="P5292_04480"
     CDS             complement(<1..605)
                     /locus_tag="P5292_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS38416.1"
                     /translation="MQRYYSTFFLLLLAFGLRAATPDSSSAFSVSQAIEYALTHQKDV
                     LNAQLDASIAKQQVNETIGIGLPQINGKFDVQDFVKIPTSVIPGEFFGEPAGTYIPVQ
                     FGTQWQANAGISASQLLFEPSYIIGVQASRTISELSQKNVKRSRIETAVAVYKAYYNH
                     LLMLERKKVIDANVERVRTLRDNTKALYDNGFVEKTDADRI"
     gene            complement(634..1602)
                     /locus_tag="P5292_04485"
     CDS             complement(634..1602)
                     /locus_tag="P5292_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRS38417.1"
                     /translation="MQPVAHQRLLREKREQFVYLFTVPTIDRRDRVQGVDFHMDGKAR
                     RYKTAYRRTIQVSTYASTPINDGSGFPMIVSTGITAETPGTILVSRWFSSLKKLAGNF
                     RTENSFHSFAPQMEQLTEPQESRIMEVSRELFFRHGLKSITMDDIAQQLGMSKKTIYQ
                     HYSDKDALIMAMVRLQTNCHEKDLLDIRKSSENPIHEILIGMNTFAATFARINPTFFF
                     DLQKYHPACWKMFKEFKDKQMIGFLEDNLRRGIRQELYRKDINVKILSRLRMSEVEIG
                     FNPQIFPPDLFTMPEVQVKIMEHFLYGIVSLKGYKLINRYKQINED"
     gene            1489..2832
                     /gene="purB"
                     /locus_tag="P5292_04490"
     CDS             1489..2832
                     /gene="purB"
                     /locus_tag="P5292_04490"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRS38418.1"
                     /translation="MEINPLNAISPIDGRYRKQVDELFPFFSEQALMRYRLQVEVEYF
                     IALVECGLPQLTAAAKLDLDGLRSIYRDFDEAGARIIKEKEAITNHDVKALEYYLKDQ
                     LDKRGFTPFLEFVHFGLTSQDVNNTAFPMSIRDALLQVYQPALDKIIQRLADLSSDWA
                     ELPMLSRTHGQPASPTRLGKEIRVFVERLQNQREQLFAIPPSAKFGGATGNFNAHCVA
                     YPEIDWRVFANAFLKNRLGLERQQYTTQIEHYDNLCALFDGMKRINNILIDLNRDVWT
                     YISLDYFKQRIKEGEVGSSAMPHKVNPIDFENSEGNLGMANAVFEHLSAKLPISRLQR
                     DLTDSTVLRNVGVPFAHSLIAFKSLLRGLVKLLVNEAALKADLENNWAVVAEAIQTVL
                     RREGVANPYEQLRDLTRKNERIGKEQLHAFIDQLPVSDNIRKELKAVTPFNYTGV"
     gene            2835..3707
                     /locus_tag="P5292_04495"
     CDS             2835..3707
                     /locus_tag="P5292_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS38419.1"
                     /translation="MFVSGFTFIRNAVRFDYPIVEAIRSILPLCDEVVVAVGRSDDNT
                     RELIEQIGSDKIRILDTVWDDALREGGRVLAEETNKAFAAVNPKADWCFYIQGDEVIH
                     ERDHPAIRQEMLQWKDDPRVEGLLFRYLHFYGSYDYVGDSTRWYRREVRIVRRDPAIV
                     SFRDAQGFRKSGRPLWVKPVDAVVHHYGWVKPPEQQQAKQRSFHALWHSDEWVRDRVG
                     TNSTFDYSGIDSLAPFKGSHPAVIRERIQRQNWTFDFDPSRRRLSIKSKFKLWVEHLT
                     GWLPGEYRNYRLLR"
BASE COUNT          901 a          993 c         1021 g          947 t
ORIGIN      
        1 tcgatccgat cggcatccgt tttctctaca aagccgttgt catagagcgc cttggtattg
       61 tcccgcaggg tacgcacgcg ttcaacattg gcatcgatca ccttcttgcg ctcaagcatg
      121 agcagatggt tgtagtaggc cttgtaaacg gcgacggcgg tctcgatccg tgatcgtttg
      181 acgttctttt gtgaaagttc gctgatggtc cggctggcct ggactccgat gatataggaa
      241 ggttcaaaca gcagctggct ggccgaaatg ccggcattgg cttgccattg tgtgccgaat
      301 tgtaccggaa tgtaggtgcc ggctggttcg ccgaagaatt cgccggggat cacgctggtc
      361 gggatcttga cgaagtcttg cacatcgaac ttgccgttga tctgcggaag accgatgccg
      421 atcgtctcgt tgacctgctg tttcgcgatg gaagcgtcaa gctgggcgtt gagtacatct
      481 ttttgatgcg tgagggcgta ctcgatcgcc tggctcaccg agaaagcgga tgaactgtcg
      541 ggtgttgccg cccgaaggcc gaaggccaac agcagcagaa agaaggttga ataatagcgt
      601 tgcataaggt tgggagtgaa ggagggaggg ggattagtct tcgttgattt gcttataacg
      661 gttgatgagc ttgtagcctt tgagactgac aattccataa aggaaatgtt ccatgatctt
      721 gacttggact tccggcatgg tgaacaggtc gggaggaaaa atctgcgggt taaaaccgat
      781 ttcgacttca ctcatgcgca agcgggaaag tatcttgaca ttgatgtctt tacgatagag
      841 ctcctgccga ataccccgcc gtaaattatc ctccaggaat ccgatcatct gcttgtcttt
      901 gaattccttg aacatcttcc aacaagcagg atgatacttc tgcagatcga agaaaaaggt
      961 gggattgatc cgggcaaacg ttgcggcaaa cgtattcatg ccgatcagaa tttcgtggat
     1021 cgggttttcg ctcgatttcc ggatatccag caggtccttt tcatggcagt tcgtttgtag
     1081 acgaaccatc gccatgatta gtgcatcctt atcggaataa tgctggtaaa tggtcttttt
     1141 tgacatgccc agttgctggg ctatgtcgtc catcgtaatg ctcttcaacc cgtgacggaa
     1201 gaacagctca cgcgagacct ccatgatccg tgattcctgt ggttcagtca gttgttccat
     1261 ttgcggggca aaactatgga aactattttc agtcctaaag tttcctgcaa gtttttttag
     1321 gcttgaaaac cagcgcgaaa ctaagatcgt tcccggcgtt tctgcggtaa ttccggtgga
     1381 aacgatcatt ggaaaccccg atccgtcatt aatcggggtt gaagcgtaag ttgaaacctg
     1441 aattgtccgt cgataggccg ttttgtacct tcgcgcttta ccatccatat ggaaatcaac
     1501 cccctgaacg cgatctcgcc gatcgatggt cggtaccgta aacaggtaga cgaactgttc
     1561 ccttttttct ctgagcaggc gctgatgcgc taccggctgc aagtggaggt tgagtatttc
     1621 atcgcattgg tggaatgcgg tttgccacag ttgaccgcgg ccgctaaact cgatttggac
     1681 ggactccgtt ccatttatcg ggactttgat gaagccggag cgcggatcat caaggaaaag
     1741 gaagcgatca cgaatcacga tgttaaagcc ctggaatatt atctgaagga tcagcttgac
     1801 aaacggggat tcaccccctt tctcgagttc gttcacttcg gcctgacctc ccaggacgtg
     1861 aacaataccg ccttcccgat gtccatccgg gatgcactgc tccaggtcta tcagccggca
     1921 ctggacaaaa tcatccaacg actggccgat ctttcgtccg attgggcgga acttcccatg
     1981 ctttcccgta cccacgggca acccgcatcg ccgacccgcc tgggaaaaga gatccgcgta
     2041 ttcgtagagc gattgcagaa ccagcgtgaa caactgttcg ccatcccgcc ttcggccaag
     2101 ttcggcggag ctaccggcaa cttcaacgcg cattgcgtcg cctatccgga gatcgactgg
     2161 cgggtattcg cgaacgcgtt cctcaaaaat cgtctcggtc tggagcgtca acaatacacc
     2221 acccagatcg agcactacga taatctgtgt gcgctctttg acggcatgaa gcggatcaac
     2281 aacatcctga tcgatctgaa ccgggatgtc tggacctata tctccctcga ttatttcaag
     2341 cagcgaatca aggaagggga agtgggctcg tcggctatgc cgcataaggt gaacccgatc
     2401 gattttgaaa attcggaagg gaacctgggc atggccaatg cggtgttcga gcatctctca
     2461 gctaagctcc cgatttcccg actgcaacgc gacctcaccg attcaacggt gttgcgcaac
     2521 gtcggcgttc ccttcgcgca ttccctcatc gcgtttaagt ccctgctccg cgggcttgtt
     2581 aaattgctgg tcaatgaagc tgccctgaaa gcggacctgg aaaacaactg ggccgtggta
     2641 gccgaggcga ttcagaccgt actgcgccgt gaaggagtag ccaacccgta cgaacagttg
     2701 cgcgacctga cccgcaagaa tgagcgcatc ggaaaggagc agttacatgc tttcatcgac
     2761 cagttaccgg taagcgataa catccgaaag gaactcaaag cggtgacccc ctttaactat
     2821 acgggagtct gaacatgttt gtcagcggtt tcacgttcat ccgcaacgcg gtccgcttcg
     2881 actacccgat cgttgaggct atccggtcca tccttcctct ctgtgacgag gtggtagtgg
     2941 cagtcggtcg ctccgacgac aatacgcgtg aattgatcga acagatcggt tcggataaga
     3001 tccgtatcct ggataccgtt tgggatgatg ctctccgcga aggaggtcgg gtactcgccg
     3061 aggagaccaa taaggctttt gctgccgtca atccgaaggc cgattggtgt ttttatatcc
     3121 agggcgatga ggtgatccac gagcgggacc atccggcgat ccggcaggag atgttgcagt
     3181 ggaaggacga tccacgcgtg gaaggattgc tgttccgcta ccttcacttt tatgggagct
     3241 acgactatgt aggcgattct acgcgttggt atcgtcgcga agtcaggatc gtccgccgtg
     3301 accctgcgat cgtttcgttt cgcgacgcac agggttttcg gaaatccggc cggccgttat
     3361 gggtgaaacc ggtcgatgcc gtagtgcatc attacggctg ggtgaagcca ccggaacaac
     3421 aacaggctaa gcagcggagt ttccatgccc tttggcattc cgacgaatgg gtacgcgatc
     3481 gggtgggtac caatagcacg ttcgattact ccgggatcga ttccctggca ccgttcaaag
     3541 ggagccatcc ggcggttatc cgcgaacgta tccaacgcca aaactggacg ttcgatttcg
     3601 atcccagccg tcgccggcta tcgatcaaat caaaattcaa attgtgggta gaacacctca
     3661 ccggctggct tcccggcgaa taccgcaatt accgactact tcgctaatat gcagaagagc
     3721 tgaaactgag ccaggaaata actttttcaa atgagaataa agacttaaaa atcaatagat
     3781 tatatatcgc atccttcacc tcaattcacg ccatcttttt caaactctta atttctaacc
     3841 cccagtctct aattactaat ca
//
LOCUS       DAOTON010000168         3850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18978_length_3850_cov_3.122003, whole genome shotgun sequence.
ACCESSION   DAOTON010000168 DAOTON010000000
VERSION     DAOTON010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18978_length_3850_cov_3.122003"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /gene="dnaG"
                     /locus_tag="P5292_04500"
     CDS             complement(<1..1316)
                     /gene="dnaG"
                     /locus_tag="P5292_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRS38420.1"
                     /translation="MIARETIDRILDAVRIDEVVGDFVQLKKRGTNLLGLCPFHNEKT
                     PSFNVNPVRNIYKCFGCGKGGDAVNFVMEHEHYSYPEALRYLAKRYNIEIEETAPDPQ
                     EAQLRDERESLYILNSYAQRVFSEQLFEDEEGRAIGMSYFRERGFTEETIKTFQLGFN
                     RSDWSAFAERAVKEGYKEEYLEKTGLCIRKDNGKLFDRFRGRVMFPIHNLSGRVIGFG
                     GRVLKKDDKTAKYLNSPESEIYHKSKSLYGIYFAKKAIVQLDNCYLVEGYTDVISLHQ
                     AGIENVVASSGTSLTSEQIRLIGRYTKNITVLYDGDAAGIKASLRGIDLILEEGLNVK
                     VVLFPDGDDPDSYTRKHGSSSTVDFIEANAKDFVLFKTGLLLDDVKNDPVRKAGLIRD
                     VVETIGHIPDPILRSTYTKQCSALLDIQENILVSELNKIRRNQLKK"
     gene            complement(1313..2317)
                     /locus_tag="P5292_04505"
     CDS             complement(1313..2317)
                     /locus_tag="P5292_04505"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRS38421.1"
                     /translation="MNLRKSYYLLSTLLLLGAAFLALSPSFVQPDFILPKRVDSLFVI
                     YETETAHRVNQQADTLPLAAPLFNPGELGLAYEQLRVYTSDSIVLHGWYVPCADTNAN
                     TLLLVHDLNDSKLRLLDAVKQFHDRGLHVAVFDLRAHGESEGKIFSPGMIAVEDLKHV
                     TDALLRRAETKHICLMGYGIGSAIVLQSAVVDGRADALVLQSPFANLENYLARYAYAK
                     WGRLKPLWYPVFHRKTEQLLGHPVEAMDLPLLASVVKTPTLVVTGSEDELVFPTESLA
                     VFDSSAAPKKDLLLIRKAGHQNIDELGGADYYNRIAAFLVTAVPRKQQKTRFKRLAQL
                     "
     gene            complement(2317..3294)
                     /locus_tag="P5292_04510"
     CDS             complement(2317..3294)
                     /locus_tag="P5292_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRS38422.1"
                     /translation="MSQKLDEIKAPIAPVMEEFERKFRESMKSPVPLLDRITHYIVKR
                     KGKQMRPMFVFLSAGLHGPVGDTSHRAATLIELLHTATLVHDDVVDDSNERRGFFSLN
                     ALWKNKIAVLVGDYLLSKGLLLSIGNKDFRLLELVSEAVKVMSEGELLQIEKARRLDI
                     TEDIYYEIIRRKTASLIAACCACGASSAGASDETVQQMHRFGELIGIAFQIKDDLFDY
                     GPEGVIGKPTGIDIKESKMTLPLIHALSKATQADKKRIIHIIKNHNTDNKKVREVIRF
                     VESSGGITYATERMYHYQEEAFRLLDTFPANAYRDSLRSLVRYTTERPL"
     gene            complement(3634..>3850)
                     /locus_tag="P5292_04515"
     CDS             complement(3634..>3850)
                     /locus_tag="P5292_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38423.1"
                     /translation="PVPARERVYLRAHVRTQGYTYTVLDLLGRTILPTQALQPDTYLD
                     RATIGSGTFFVIIGNGEWREVERVVVE"
BASE COUNT          653 a         1123 c         1217 g          857 t
ORIGIN      
        1 tctttcttca gttggttccg gcggatcttg ttcagctccg aaacgaggat gttctcctgg
       61 atgtcgagca gggcgctgca ctgcttggtg taggtcgaac gcaggatcgg atcggggatg
      121 tgtccgatcg tttccaccac gtcgcggatg agcccggctt tgcgtaccgg gtcgttcttc
      181 acgtcgtcaa gcagcaggcc ggtcttgaac aggacgaaat ccttggcgtt cgcttcgatg
      241 aaatcgaccg tcgaactgct gccgtgctta cgcgtatagg aatcgggatc gtcgccgtcg
      301 gggaagagga cgaccttcac gttcagtcct tcttcgagga tcagatcgat cccgcgcagc
      361 gaggccttga tgcccgccgc gtcgccgtcg tacaggacgg tgatgttctt cgtataacga
      421 ccgatcagcc ggatctgctc gctggtgagc gaggtgccgc tgctggccac cacgttctcg
      481 atgcccgcct ggtggagcga gatcacgtcg gtatagcctt cgacgagata acagttgtcg
      541 agctgtacga tcgccttctt ggcgaagtag atcccgtaga gcgatttgct cttgtggtag
      601 atctccgact cgggcgagtt gaggtacttg gcggtcttgt cgtccttctt cagcacgcgc
      661 ccgccgaaac cgatcacgcg accgctcagg ttgtggatcg ggaacatgac gcgtccgcgg
      721 aagcggtcga acagcttgcc gttgtccttg cggatgcata gcccggtctt ttcgaggtac
      781 tcttccttgt acccttcctt caccgcgcgc tccgcgaacg cgctccagtc ggagcggttg
      841 aagccgagtt gaaaggtctt gatcgtttct tcggtgaatc cgcgctcacg gaagtaggac
      901 atgccgatgg cacggccttc ctcgtcctcg aacaactgct cggaaaagac gcgctgggcg
      961 tagctgttca ggatgtagag gctttcgcgt tcgtcgcgca gttgcgcttc ctgcgggtcg
     1021 ggcgcggtct cttcgatctc gatgttgtag cgcttggcca ggtaccgcag ggcttccggg
     1081 tagctgtagt gctcgtgctc catcacgaag ttcaccgcgt cgccgccctt gccgcagccg
     1141 aaacacttgt agatgttgcg gaccgggttg acgttgaacg aaggggtctt ctcgttgtgg
     1201 aacgggcaaa ggccgagcag gttcgtgcca cgctttttaa gctggacgaa atcgccgacc
     1261 acctcatcga tgcggacggc gtcgaggatc cggtcgatgg tctcgcgcgc gatcatagct
     1321 gcgccagtcg tttgaaacgc gttttctgct gttttctggg aaccgcggtc accaggaacg
     1381 cggcgatgcg gttgtaatag tccgccccgc ccagttcgtc gatgttctgg tgccccgcct
     1441 tgcgtatcaa aagtaagtct tttttcggag cggcggagga gtcgaaaacg gccagggatt
     1501 cggtcgggaa gaccagctcg tcctcgctgc cggtcaccac cagggtgggt gttttcacca
     1561 cgctggcgag caggggcagg tccatggctt cgacggggtg gcccagcaat tgttccgttt
     1621 tccggtggaa aacagggtac caaaggggtt tcagccgtcc ccacttcgcg taggcatagc
     1681 gggccaggta gttctcgagg ttggcgaacg ggctctgcaa cacgagcgcg tcggcgcgtc
     1741 cgtcgaccac ggccgactgc aacacgatgg ccgaaccgat cccgtagccc atcaggcaga
     1801 tgtgcttcgt ctccgcacgt cgcagcaggg catcggtcac gtgcttcagg tcttccaccg
     1861 cgatcatgcc gggcgagaag atctttcctt cgctctcgcc gtgcgcgcgc aagtcgaaca
     1921 ccgccacgtg caggccgcgg tcgtggaact gtttcaccgc gtcgagcagc cgcagcttgc
     1981 tgtcgttgag gtcgtggacc aacagcaagg tattcgcgtt cgtatccgcg cagggcacat
     2041 accagccgtg caggacgatg ctgtcgctgg tgtagacgcg cagttgttcg tacgccagtc
     2101 ccagttcgcc gggattgaag agcggcgcgg ccagcggcag ggtgtcggct tgttggttga
     2161 cacgatgtgc cgtttcggtt tcgtaaatga cgaaaagcga atcgacgcgc ttgggcagga
     2221 tgaaatccgg ctgcacgaac gaaggcgaca gggcaagaaa ggccgcgccg aggagcagca
     2281 gggttgacag caggtagtac gacttgcgca ggttcatcag agcgggcgtt cggtggtgta
     2341 acgaacgagg ctgcggagcg aatcgcggta ggcgttggcc ggaaaggtgt cgagcaggcg
     2401 gaaggcttcc tcctgataat ggtacatgcg ttcggtggcg taggtgatgc ctcccgagct
     2461 ttccacgaag cggatgactt cgcgtacctt cttgttgtcc gtgttgtggt tcttgatgat
     2521 gtggatgatc cgcttcttgt cggcctgggt ggccttcgac agcgcgtgga tcagcggcag
     2581 ggtcatcttg ctttccttga tgtcgatgcc ggtcggtttg ccgatcacgc cttcggggcc
     2641 gtagtcgaag aggtcgtcct tgatctggaa ggcgatgccg atgagttcgc cgaagcggtg
     2701 catctgctgc acggtctcgt cgctcgcccc ggccgaggaa gccccgcagg cgcagcatgc
     2761 ggcgatgagc gaagccgtct tgcggcggat gatctcgtag tagatgtcct cggtgatgtc
     2821 gagccgccgg gctttttcaa tctgcagcag ctcgccctcg ctcatcacct tcacggcctc
     2881 ggagaccagc tcgagcaggc ggaagtcctt gttgccgatg gacaacaaca ggcccttgct
     2941 cagcaggtag tcgcccacca gcaccgcgat cttgttcttc cagagcgcgt tcaggctgaa
     3001 gaagccccgg cgctcgttgg agtcgtcgac cacgtcgtcg tggacgagcg tggccgtgtg
     3061 cagcagttcg atgagcgtgg cggcgcggtg gctggtatca cccaccggtc cgtgcagtcc
     3121 ggccgagagg aacacgaaca tcggccgcat ctgcttgccc ttgcgcttga cgatgtagtg
     3181 cgtgatgcgg tcgagcagcg gcaccggact cttcatcgac tcccggaact tccgttcgaa
     3241 ctcctccatc accggcgcga tcggcgcctt tatttcgtcc agtttttggg acatagcagg
     3301 cggcaaaggt acggattggc ggtttgcttc ttagcgaata gcgaatagcg aacagcgaat
     3361 agttttttta gtgaacagta agtagcgaat agcgaatagc gattagcgaa tagtttttgg
     3421 taacagccag agccgaaacc gcatccattt tctcgctcct cgctcctcgc atctaaccct
     3481 cggtctcgtc ccacgtccca cgtccctcgt ccctcgtccc tccttcctcg gtctcggtct
     3541 cgttcctcgc aactctactt cgtccctcgt ccctcgtccc tcgtccctcc ttcctcggtc
     3601 tcggtctcgt tcctcgcaac tctatctcgt ccctcactcc accaccaccc gctcaacctc
     3661 ccgccactcc ccattcccga tgatgacgaa gaacgtgccg ctgccgatgg tggcgcggtc
     3721 gaggtaggtg tcgggttgga gggcttgggt ggggaggatg gtgcggccga ggaggtcgag
     3781 tacggtgtag gtgtagccct gggtgcgcac gtgcgcgcgg aggtacacgc gctcgcgcgc
     3841 gggcacgggg
//
LOCUS       DAOTON010000169         3850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_18979_length_3850_cov_2.526482, whole genome shotgun sequence.
ACCESSION   DAOTON010000169 DAOTON010000000
VERSION     DAOTON010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18979_length_3850_cov_2.526482"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..748)
                     /locus_tag="P5292_04520"
     CDS             complement(44..748)
                     /locus_tag="P5292_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRS38424.1"
                     /translation="MKRHPRLVVLSGAGMSADSGIRTFRGADGLWENYRIEDVATPMA
                     WQRDPELVQRFYNERRKQVLEAQPNEGHLALARLEACFDVRIITQNIDDLHERAGSSS
                     VLHLHGEIRKSRSSLDPSLTYPIEGWELRMGERCAKGSQLRPHIVWFGEMVPLLPEAA
                     HLVSTADVFLVVGTSLAVYPAASLVDYVPVAAERFVLDPEIPDHLMGYTAIRSSAASG
                     LPTLALQLIETYTTKS"
     gene            complement(810..1784)
                     /gene="fmt"
                     /locus_tag="P5292_04525"
     CDS             complement(810..1784)
                     /gene="fmt"
                     /locus_tag="P5292_04525"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRS38425.1"
                     /translation="MKHTHPRIVFMGTPEFAVPSLEALVEAGFPVVGVVTAPDKPAGR
                     GQQLQASAVKQAALRLGIPVLQPEKLRSPEFLEQLKSLQAELQIVVAFRMLPEVIWSA
                     PPMGTFNLHASLLPQYRGAAPINWAIIQGERFTGITTFFLRQDIDTGNILLQEKVEIG
                     TDETVGSLYARLMHAGAGLVVRTVQGLMDKSITPRPQADTSLDGSPFKPAPKLNKENT
                     RLSTALPVASQFNFVRGLSPYPAAHGEFFRLSDQRSIPVKLFQVRASPDDKSHLSGKL
                     LVQDKEKLFIEGPDGLLEILELQPAGKGRMKARDFLNGNRLEGEWTFR"
     gene            complement(1781..3076)
                     /locus_tag="P5292_04530"
     CDS             complement(1781..3076)
                     /locus_tag="P5292_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRS38426.1"
                     /translation="MAYPVMLGSFATTILNVTDSVFLGRIGEVELGASALGGILYFVF
                     AMIGVSIGTGTQILIARRTGERNDAGVGPIFDHSFLLLAGISILLFLILFVGAPVFLP
                     YMVRNPKILAAAIAFLEYRSFGIFFIMIAAVYRSFYVGIAQPKVYGTYSFLMAGLNLL
                     LNYTLIFGHWGFPEMGIAGSGLASSISEAVALFYLVFYTSGKRSLNIYQLFKFKSIQS
                     SVFRSTLELSIPLIVQNLLSMGAWFIFFIFIEKIGQHELAISNMVRGAYMVSMTPIWG
                     FAIAANSMVSNVIGQDRREEVMVLLNRIILLAFGTAAVMVAINLLFPVQLLGAFTQNQ
                     QLIEDARPSLMVVNVSMFFFAFAMVCISAVSGTGATRTALYIEIAAILLYMVYNYLVT
                     FVFSGTVEAVWMSEIIYWVFTGLASYQFLRSSRWKAIQL"
     gene            complement(3172..>3850)
                     /locus_tag="P5292_04535"
     CDS             complement(3172..>3850)
                     /locus_tag="P5292_04535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008563781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRS38427.1"
                     /translation="ELQDGLIDGPEPPRPLFIEENGLRYGIDIVEGQKTGFFLDQREN
                     RELIRTYAHERDVLNTFSYSGGFSMYAGLAGARSVVSVDSSRKAIEACNRNAELNGLQ
                     QHASSCSDVFDFLKHQSEQQFDLIVLDPPAFAKHLSSVENAMIGYRNLNTEGFRRIKS
                     GGILFTFSCSQVMDKLLFRKVVFQAALQARRTVRILHQLSQGPDHPINVYHPEGEYLK
                     GLVLYVS"
BASE COUNT          987 a         1028 c         1014 g          821 t
ORIGIN      
        1 aatactaccc aacggaaaaa ctcggtatca atgaaagctg aattcatgac ttggttgtat
       61 aggtctcgat caattgaagc gccaacgtag gcaagccgga tgcagcactg gaacggatgg
      121 cggtataacc catcaagtgg tccggaattt ccggatcaag aacgaatcgt tctgctgcta
      181 ccggcacgta atcgaccagg ctggcagcag gataaacggc cagtgacgtt ccaacgacca
      241 gaaaaacatc ggctgtggaa acaaggtggg cagcctccgg caataacggc accatctcac
      301 cgaaccagac tatgtgcggc cgcaactgcg acccttttgc acaacgttct cccatgcgca
      361 actcccaacc ctcgatcgga taggtcaagg aggggtccag ggacgacctt gatttgcgga
      421 tctctccatg caagtgcaac acactgctcg acccggcacg ttcgtgtaag tcatcgatgt
      481 tttgggttat gatcctgaca tcaaaacacg cttccaatcg tgcaagcgcg aggtgaccct
      541 cattgggttg agcttccagt acctgtttgc ggcgctcgtt ataaaaacgc tgaacaagtt
      601 ccggatcccg ctgccaggcc atgggagtag ccacgtcttc gatccggtag ttttcccaaa
      661 gaccgtctgc gccccgaaaa gtgcgaatac cactgtcggc actcatgccg gcaccggaca
      721 atacgaccag tcgtggatgc cttttcatgc tgcaaagttc ctgactgccg caatgcgctc
      781 gactggcaat gctgtttttt ttttcacggc tatcgaaagg tccattcacc ttccagtcga
      841 ttgccattca gaaaatcacg ggccttcatc cgacctttcc cggccggttg aagttccagg
      901 atctccaaca agccatcggg accttctata aacagcttct ctttgtcctg taccagcaac
      961 tttccggaaa gatgcgactt gtcatcagga gaggccctga cctgaaagag ttttaccggt
     1021 attgaacgct ggtccgatag tcggaaaaac tctccatgcg cagccggata gggggaaagt
     1081 ccccggacga aattgaattg cgacgcaact ggtagcgcgg tggacaaacg agtattttcc
     1141 ttgttcagct tcggtgcggg cttgaatgga gatccatcca gcgacgtgtc tgcctgcggt
     1201 cggggagtga tgctcttgtc catcagcccc tgcaccgtcc tgaccacgag tccggcgcct
     1261 gcgtgcatca gacgcgcgta gagggaacct acggtttcgt cggttccgat ctccactttt
     1321 tcctgcaaca agatattacc ggtatcgatg tcctgccgga gaaaaaaggt cgtaataccg
     1381 gtaaaacgct caccttgtat gatggcccaa ttgatcggtg cggcaccgcg gtattgggga
     1441 agtaaagacg cgtgtaaatt gaaggttccc attggaggag ccgaccatat cacctcgggt
     1501 aacattcgaa aggccacgac aatctgcagc tctgcctgga gggatttcaa ttgttccagg
     1561 aactccggag atcgtagctt ttcgggttgc aataccggga tgcccaaacg aagtgccgcc
     1621 tgcttgacag cggatgcctg aagttgctga ccacggccgg caggtttgtc gggggctgtc
     1681 actactccaa ccactggaaa tcccgcctcc accagtgcct ccagggaagg gacggcaaac
     1741 tcgggagttc ccatgaatac gatcctgggg tgcgtgtgct tcataattgg atggctttcc
     1801 atcgtgagga acgtaaaaac tggtaagaag ccagaccggt gaatacccag taaatgattt
     1861 ccgacatcca cacggcctcc accgttccgg aaaaaacaaa ggtgacaaga taattgtata
     1921 ccatgtacaa caggatggcg gcaatctcga tataaagggc ggtgcgggtc gcccccgttc
     1981 cgctgacagc gctgatgcag accatggcaa atgcgaaaaa gaacatcgaa acattcacca
     2041 ccatcagact tggtcgcgcg tcctcgatga gctgttggtt ctgcgtaaag gcccccagta
     2101 gctgaacggg aaacagcagg ttgatggcga ccatgactgc agcggttccg aatgcaagca
     2161 gaatgatcct gtttaggagc accatgactt cctcgcgccg gtcttgtccg atgacgttgc
     2221 tgaccatgga attcgccgcg atggcgaagc cccatattgg ggtcatgctg accatgtagg
     2281 caccacggac catattgctg atggccagct cgtgttgccc gatcttttcg ataaaaatga
     2341 aaaagatgaa ccaggctccc atggaaagga ggttttgaac tatcagcggt atcgacaact
     2401 caagtgtgga acgaaagacg gacgactgaa tggatttgaa tttgaaaagc tgatagatgt
     2461 tcagggatcg tttgccgctc gtatagaaga ccagatagaa caacgcgaca gcttccgaga
     2521 tactggaagc aagtcctgat ccggcaatcc ccatttcagg aaagccccaa tgcccgaaga
     2581 tcagtgtgta attcagtaac aggttcaacc ctgccatgag aaaactgtag gtcccataga
     2641 ctttgggttg cgcgatgccg acatagaagc ttcgatagac cgcagcgatc atgatgaaga
     2701 aaataccgaa agaccgatac tccaagaagg cgatcgctgc cgccaggatc ttggggttcc
     2761 ggaccatata gggcaggaag accggagctc ctacaaacaa gatcaggaac aacaggatgc
     2821 ttattcccgc aagcagtaga aaactgtggt cgaagatcgg cccaaccccg gcatcgttcc
     2881 tttcaccggt acgacgcgcg atcagtattt gcgttccggt accgatggag acaccgatca
     2941 tcgcaaagac gaaatacagg ataccgccaa gcgcagaagc gcccagttcc acctcgccga
     3001 ttcgtccaag gaataccgaa tcggtaacgt tgaggatcgt ggtcgcgaag ctgcccagca
     3061 tgaccggata ggccatacgg aggatgtcct tgtatgtcgt attaacctgc aaaacgaaaa
     3121 ttcgggagga cgaaggtact caaatactca gaggaggcag gagggaagcc gtcaggaaac
     3181 gtacaagacc agtcctttca ggtactcacc ttccggatgg tagacattga tcgggtgatc
     3241 gggtccctga ctgagttgat gcaggatgcg gaccgttctg cgcgcctgta aagccgcttg
     3301 aaaaactact ttccggaaga gcaatttgtc catcacctga gaacaagaaa aggtgaacag
     3361 gatgccgccg gatttgattc gtcggaaccc ttcggtgttc agattgcgat aaccgatcat
     3421 ggcattctca accgaactca ggtgcttggc aaatgccggc ggatccagta cgatcaggtc
     3481 gaattgctgc tcggactggt gcttaagaaa gtcgaacaca tcggaacacg aagaagcgtg
     3541 ttgctgtagt ccgttcagct ccgcattgcg gttacaggct tctatcgctt tcctagaact
     3601 gtcaacggaa acaaccgacc gcgctcccgc caaaccggca tacatcgaga atccacccga
     3661 gtaactgaag gtgttcagga catcgcgctc atgggcataa gtgcgtatga gctcccgatt
     3721 ctcccgttga tccagaaaga aaccggtctt ctgcccctcg acgatatcga tcccgtaccg
     3781 caatccgttc tcttcgatga acaacggtcg cggcggctcg ggaccatcga tcagcccatc
     3841 ctgcagttcc
//
LOCUS       DAOTON010000170         3841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19035_length_3841_cov_2.560486, whole genome shotgun sequence.
ACCESSION   DAOTON010000170 DAOTON010000000
VERSION     DAOTON010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19035_length_3841_cov_2.560486"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /gene="rho"
                     /locus_tag="P5292_04540"
     CDS             <1..936
                     /gene="rho"
                     /locus_tag="P5292_04540"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRS38428.1"
                     /translation="EGEKYFPLIRAELINGLDPAVVRDRVHFDYLTPLFPNEKLNLAE
                     GKQVTYSTRIMDMFTPIGKGQRGLIVAQPKTGKTMLLKEVANAIASNHPEVYMIILLI
                     DERPEEVTDMARSVKAEVVASTFDEPAERHVKIANIVLEKAKRLVECGHDVVILLDSI
                     TRLARAYNTVAPASGKVLSGGVEANALHKPKRFFGSARKIENGGSLTIIATALIDTGS
                     RMDEVIFEEFKGTGNMELQLDRKLANKRIFPAIDLVASSTRREDMLLDKEMLQRMWIL
                     RNHLADMNPLEAMEFLLQQLRGTKTNEEFMISMRG"
     gene            1006..2568
                     /locus_tag="P5292_04545"
     CDS             1006..2568
                     /locus_tag="P5292_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRS38429.1"
                     /translation="MRDLARQRRQRYQRKLFLRHDLPAGLYVFLVALPLCLGIALASG
                     APLYAGIISGIVGGLFVSLVSGSALGVSGPAAGLTTIVAASIAIHGDFRSFLFAVMIA
                     GLFQVGLGVLRLGTIARFFPSAVIKGMLAAIGILLISKQIPVSIGYNQPDFWSSGFLE
                     LFQSKHVLSNLLDLNEHISRSAIIITLCSLGTLALFRRLQQPWAKSIPAPLLAVLIGI
                     MCNELFSLLDSGALKPTQLVNIPSNLFQEFQWPDYHRISSWEQTIRDGLLIGLVASLE
                     TLLCVEAIDKLDIHNRITPPNRELIAQGTGNILCGVLGGLPMTAVIVRGAANVQAGAR
                     SRWSSFMHGVFLLLAVLLIPSLLNRIPYAALAAILLFTGYNLTPPRLYRNIWRQGMTQ
                     FLPFILTIVVILLTDLIIGVSIGLLLSVYFIVQNNFQAEFRLERTKRHETDHVYITLN
                     TNVTFLNKVKIAQLLDKIEPYTVLTIDGANSNFIDYDVREVISDFALKAKERHIELHL
                     IGIETVQVDFMH"
     gene            complement(2630..>3841)
                     /locus_tag="P5292_04550"
     CDS             complement(2630..>3841)
                     /locus_tag="P5292_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS38430.1"
                     /translation="KTYIGAMPGRIIQNLRKTKSSNPVFVLDEVDKLGNDFHGDPSSA
                     LLEVLDPEQNNSFYDNYLEVDYDLSNVLFIATANSLSRVQPALLDRMEIIEINGYTLE
                     EKVQIAIRHLLPKQLEAHGLKKDQLKLSPKVIEHIIERYTRESGVRGLEKKIASVVRA
                     IAKSVAMEEEYELAVSVEAVGKILGPPIFQQDKYQGNENAGVVTGLAWTAAGGDILFV
                     ETSLNRGQGKLSITGNLGEVMKESAVIAMAFLKAHAQELGIDYRVFEQWDIHIHVPEG
                     ATPKDGPSAGITILTALTSAFTQRKVRSNLAMTGEITLRGKVLPVGGIKEKMLAAKRA
                     GIKDIILSEENRKDVEDIRKDYIKDLRFHYVSDMMDVVRYALLKEKVRDALDIRFEDN
                     GQEKKKKVNAS"
BASE COUNT          807 a         1115 c          992 g          927 t
ORIGIN      
        1 gaaggtgaaa agtacttccc cctgatccgg gcagaactca tcaacggtct ggatcccgca
       61 gtggttcgcg atcgggttca cttcgattat ctgacaccgc tcttcccgaa cgaaaaactc
      121 aacctggccg aaggcaagca agtaacctat tccacgcgta tcatggatat gttcacgccg
      181 atcggtaagg gtcagcgcgg actcatcgtc gctcagccga agaccggtaa gacgatgctg
      241 ttgaaggaag tcgccaacgc cattgcgtcg aatcacccgg aagtgtacat gatcatcctg
      301 ctgatcgacg aacgtccgga agaagtaacc gacatggccc gcagtgtaaa agcggaagtc
      361 gtcgcctcca cttttgacga acctgcagag cgtcacgtca agatcgccaa catcgtgctg
      421 gagaaagcca agcgcctggt ggaatgtggc catgacgtcg tcatccttct ggactccatc
      481 acccgacttg cccgtgcata caatacggta gcccctgcat ccggaaaagt gctctccggc
      541 ggcgtcgaag cgaatgcgct ccacaaaccg aagcgcttct tcggttccgc gcgaaagatc
      601 gaaaacggcg gaagcctcac gatcatcgcg acggccctga tcgataccgg atcccgtatg
      661 gacgaagtca tcttcgagga attcaaaggt accggtaaca tggaattgca gcttgaccgg
      721 aaacttgcca acaagcgcat cttcccggca atcgacctgg tcgcttcctc cacccgacgc
      781 gaggatatgc tcctcgacaa ggaaatgctt cagcgcatgt ggatcctgcg caatcacctt
      841 gccgacatga atccgctgga agcgatggaa ttcctcctgc aacagctccg gggaacaaaa
      901 acaaacgaag aattcatgat ctccatgaga ggttgatctt ctgccccgcc ctcgagcggg
      961 gctttttttt attcatcaag acaacggtct tactacactt gtccaatgcg tgatctggcc
     1021 cgacaacgaa ggcagcgcta tcaacgcaag ctctttctcc ggcacgacct tcccgccgga
     1081 ttgtatgtat tcctggtcgc gctgccactc tgccttggca ttgcccttgc ctccggcgca
     1141 cccttgtatg ccggaataat ctccggcatc gttggcggac tctttgtttc actcgtgagc
     1201 ggatccgccc tcggcgtttc tgggccggca gcgggtttga ccaccatcgt tgcagcctcc
     1261 attgccattc atggcgactt ccgatctttc ctcttcgcgg tcatgatcgc cgggctgttc
     1321 caggtgggtc ttggcgtgct gcgcctgggt acgatcgccc gtttctttcc atccgcggtc
     1381 atcaaaggga tgctcgccgc catcgggatc ctcctgatca gcaaacagat cccggtttcc
     1441 atcggttaca atcagcccga tttctggtcg agtgggtttc tcgagctctt ccagtcgaaa
     1501 cacgtgttgt cgaacttgct tgatttgaac gaacacatca gccgcagcgc gatcatcatc
     1561 accctttgca gcctgggaac gcttgccttg ttccgcaggt tgcaacaacc ctgggcgaag
     1621 agcattccgg ctccactgct ggccgtgctg atcgggatca tgtgtaacga acttttctcc
     1681 cttctggata gtggggcact aaaacctacg cagttggtga atattccatc caaccttttt
     1741 caggaatttc agtggccgga ttatcatcgt atcagttcct gggaacagac gatccgggac
     1801 ggcctgctga tcggtctggt tgcttcgttg gaaacactgc tgtgtgtgga agcgatcgac
     1861 aagttagaca tccataaccg catcacgccg ccgaaccgtg aactcatcgc gcagggaacc
     1921 ggcaatatcc tttgcggggt gctgggcggg ttgccgatga ccgccgtgat cgtgcggggc
     1981 gctgccaacg ttcaggccgg ggcacggagc cgctggtcgt cgttcatgca tggcgtcttt
     2041 ttgctgctcg ccgtgctcct gatcccgtcg ctgctcaacc ggatccccta cgcggcttta
     2101 gcagccatcc tgctcttcac cggttacaac ctgacgccac cccgcctcta tcggaacatc
     2161 tggaggcagg gaatgaccca gtttcttccg ttcatcctta cgattgtcgt catcctactg
     2221 accgacctca taatcggtgt cagcatcggc ttgttattgt ccgtttattt tatcgtgcag
     2281 aataatttcc aggcggaatt tcgtcttgaa cgcaccaagc ggcatgaaac cgaccatgtg
     2341 tacatcacgc tgaatacgaa tgtcacattc ttgaacaagg tcaagatcgc gcagttgttg
     2401 gataagatcg aaccctacac cgtattgacg attgatggtg cgaacagcaa cttcatcgac
     2461 tacgatgtgc gggaagtcat cagcgatttc gcgttgaaag cgaaagaacg acacatcgaa
     2521 ttgcacctga tcggtatcga aaccgtgcag gtggacttca tgcattgatg gaaataaaaa
     2581 gccccggaat cgtacgatac tccggggctt tcaacggatt ggctctcggt caggaagcgt
     2641 tcaccttctt tttcttttcc tgcccgttgt cttcgaatcg gatgtccagc gcgtcgcgca
     2701 ctttctcttt cagcagtgca tagcgcacta catccatcat atcggaaaca taatggaagc
     2761 gcaggtcctt gatgtaatcc tttcggatat cctccacatc cttccggttc tcttcgctca
     2821 ggatgatatc cttgatgccc gcacgcttgg ccgccagcat cttctccttg attccgccca
     2881 cgggcaatac ctttccacgc aacgtgattt caccggtcat ggccaggttg ctgcggactt
     2941 tccgctgcgt gaaggctgat gtaagcgccg tcaaaatggt aatgccggcc gatgggccat
     3001 ccttgggtgt tgctccctcc ggaacatgga tgtggatgtc ccattgttcg aacacgcgat
     3061 aatcgatccc gagttcctga gcatgggcct tgaggaatgc catggcgatc acggcggatt
     3121 ccttcatcac ttccccgaga ttgccggtga tggacagttt tccctgtccg cgattcagac
     3181 tggtctccac gaagaggata tccccgcccg ctgcagtcca ggcaagtccg gtaacgacac
     3241 ccgcgttctc gttgccctgg tatttgtcct gctgaaagat cggagggcct agtatcttgc
     3301 cgaccgcttc caccgagacg gcgagttcat attcttcctc catcgcgacc gacttggcga
     3361 ttgcccgcac cacggaagcg atcttcttct ccagaccgcg tacgcccgac tcacgggtgt
     3421 agcgctcgat gatgtgctcg atgactttcg gcgacaactt tagctggtcc ttcttcaggc
     3481 cgtgcgcctc cagttgcttc ggcagcaggt ggcggatggc gatctgcact ttctcttcca
     3541 gcgtgtaccc gttgatttcg atgatctcca tccggtcaag caaggccggc tgtacccgac
     3601 tcagggaatt cgcagtagcg atgaaaagga cattggaaag gtcgtaatcg acctccaggt
     3661 agttatcgta gaacgaattg ttctgctccg gatcgagcac ttcgagtaag gcggatgatg
     3721 gatcgccgtg aaagtcgttt cccagtttgt ccacctcgtc aagtacgaag accggattgg
     3781 aggatttggt cttccgaaga ttctgaatga ttcgcccagg catggcaccg atgtaggttt
     3841 t
//
LOCUS       DAOTON010000171         3840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19037_length_3840_cov_2.743197, whole genome shotgun sequence.
ACCESSION   DAOTON010000171 DAOTON010000000
VERSION     DAOTON010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19037_length_3840_cov_2.743197"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="P5292_04555"
     CDS             <1..1089
                     /locus_tag="P5292_04555"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRS38431.1"
                     /translation="SLYAEELVSTHGFSNVYSLIYHCHPPTMIKELGEPWSVEPKLAF
                     KRSLKHQSFEGFHVEPKDDFIESRVPVLVNDDLYITLAAPRKSMSDYFYKNADADEVI
                     FIHEGSGKMKSIYGSLSFRYGDYIVIPRGTIYQLEFDGPDNRLFIVESFSAVETCKRY
                     RNTYGQLEEHSPYCERDIRRPVDLKTYDLTGDFKILIRKQGLIYPYIYATHPFDAIGW
                     DGNHYPWILSIHDFEPLTGRVHLPPPIHQTFEAHNYVICSFVPRLYDYHPQAIVVPYN
                     HSNVDSDEVLYYVDGDFMSRKSVTRGMITLHPAGIPHGPHPGTVERSIGKKETHELAV
                     MVDTFRPLWVTEEAVRIMNPDYFRSWLS"
     gene            1198..>3840
                     /locus_tag="P5292_04560"
     CDS             1198..>3840
                     /locus_tag="P5292_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38432.1"
                     /translation="MRRRLLYLLLLFGATLLQWTERAGAVALPYATDFTSDTLWTASS
                     DQGSAWELGTPAYGITTGAHSPAEAWDVALNSAVTDGTVCYLYSPAFDFNQDLNCRLS
                     FWQNRSCEDYFDGFRIDYSLDGSTWLVLGLSGDANGKNWFTRNNIEASGLPAWSGDSH
                     GWQKSEYRLSLLNNYIGSVRFRFVFTSNDSVPSAGCSIDDFSIEQASAYDVELRDLLR
                     PDRYVVAGVADSIQAIVRNVGASSINNVLIDFTTDGVMLFSENINITLGPSEEDTITL
                     QWHYTPPAGDFYLSCFLSNPSDGDRSNDTLNCSLRGLARMQLPFADEFMASPDDWATD
                     PGSTWTLVPDSSGASSFHWDDSPGGDYLTHTDASLYSPFIQWNGERNVRCSFLQQCET
                     ESGYDGFRLEYTLDGGLNWLPVGQPGDENTENWYDHAALSAFQAGPGWSGLLPEWRNS
                     RCLLSALDGWVGLVQFRFHLSTDGSNVASGVRIDDFRLEPAPGIDLSINDVSVSHRFA
                     AAAFPDSIHIRIRNTGSTDLQDPVVQLELGTLVRSFQYSGVLQPGAAADWTIDTIGLP
                     EGPSELTIQSAVPSDGDNLNDSRSLQLFGIPTRTLPFTDDLESAILFADTSANWSLGK
                     PAFSSETSAASGMNAWNLPGSGSYPDRVTAVLYTPYFNLRGWYNPRLKFNHWFNTQSG
                     TDGGRVEVSVDSGMTWSTLGQRNDPLGINWYNTAALASSGQSGWSGGSGNWSESVYWM
                     PAYSNAQALVQFRFVFQSDDSVAVDGWSIDDIRLEASPAHSVSPEAVNGLPDPINPVL
                     PAALQCRVRNSGALPVSEFDLRVLIDDSLFHQQHCTLPTPIGNGQVATIQLSSPWQAP
                     PGGHEVTVIASATSPAEAWPADDTL"
BASE COUNT          807 a         1032 c         1019 g          982 t
ORIGIN      
        1 tccctgtatg ccgaagagct tgtatccacc cacggcttct ctaacgtcta ttcactcatc
       61 taccattgtc acccgcccac gatgatcaag gagttgggtg aaccttggtc ggtagaaccc
      121 aaactggcgt tcaagcgatc cctaaaacac cagagctttg agggctttca tgtggagccg
      181 aaggatgatt tcatcgaaag ccgggtgccc gtattggtga acgatgattt gtacatcacg
      241 ctggccgccc cccgcaagtc gatgtccgac tacttctata agaacgccga tgccgacgaa
      301 gtgatcttta tccacgaagg aagcggtaag atgaaatcga tctacggttc gctttcgttt
      361 cgatacggag attatatcgt gattccgcgt ggtaccatct accaactgga attcgacggc
      421 ccggataacc gtttgttcat cgtcgagtcg ttctcggcgg tggaaacttg taaacgttac
      481 cggaacacct acggtcaact cgaggaacac tctccgtatt gcgagcggga tatacggcga
      541 ccagtggatc tgaaaaccta cgatctaaca ggagatttta agatcctgat ccggaagcag
      601 ggactcatct acccctatat ctatgccacc cacccgttcg atgcgatcgg atgggacggt
      661 aaccattatc cctggatact ttcgatccat gacttcgaac cgctgaccgg ccgggtccac
      721 ctgccacctc ccattcatca gacgttcgaa gcgcataatt atgtgatctg ttcattcgtt
      781 ccgcgattat acgattacca tccgcaggcg atcgtagtgc cgtataacca cagcaatgtg
      841 gattccgatg aggtccttta ttatgtcgat ggcgatttca tgagccggaa atccgtaacg
      901 agaggcatga tcacactgca tccggccgga attccgcatg gaccgcatcc cggaacggtt
      961 gaaagaagca tcggaaaaaa ggaaacccat gagttggcgg tgatggtcga caccttccgt
     1021 ccgttatggg ttaccgaaga ggcggttcgc atcatgaacc cggactattt ccgctcctgg
     1081 ttgtcatagg gaagtgtaag atttcagccc ttatttcggg ctgttaagca tagtttttcc
     1141 caactcacct cctccgggga cgtatcttct gtggccggca attgccgggt cgtgacgatg
     1201 cgtcgaaggc tgctttatct tttattgctc ttcggagcga cactcctgca atggacggaa
     1261 cgcgcaggtg ctgttgcctt accgtatgcg accgatttca cttctgatac actgtggacg
     1321 gcgtcttccg atcagggaag tgcatgggaa ctcggcactc cggcctatgg tattactacc
     1381 ggtgctcatt ctcccgcaga agcctgggat gtcgccctga attcagcggt taccgacggt
     1441 acggtttgct acctgtactc tccggccttt gacttcaatc aggatctgaa ttgccgcttg
     1501 agtttttggc agaaccggtc gtgtgaggat tatttcgacg gcttccggat cgactattcg
     1561 ctcgatggtt ctacctggct ggtcctcggt ctatccggcg atgccaacgg caagaactgg
     1621 tttacgcgca acaatattga ggcttcagga ttgcccgcct ggtcagggga ttcacacgga
     1681 tggcagaaat cggagtatcg gctgtcgctg ctcaacaact acatcggcag tgtgcggttc
     1741 cggttcgttt tcacctcaaa cgattctgtt ccaagcgccg gttgctccat agatgacttc
     1801 tcgatagaac aagcttccgc gtacgatgtc gaactgcggg acctgttacg acctgaccgg
     1861 tatgtcgtcg ccggcgtagc ggatagcatc caggcgattg ttcggaatgt cggggcttct
     1921 tcgatcaaca atgtcttaat cgacttcacg accgatggag tcatgctctt ctcagagaat
     1981 atcaatatca ccctggggcc atcagaggaa gataccatca ctttgcaatg gcactataca
     2041 ccgccggctg gagattttta cctgagttgc tttctttcta atccttccga tggcgatcgg
     2101 tccaatgata ctttaaattg ttcgcttcgg ggactcgcac ggatgcaatt gccttttgcc
     2161 gacgaattca tggcaagccc ggatgactgg gctaccgatc cgggttccac ctggacgttg
     2221 gttccggatt cttcgggtgc ttcttccttt cattgggatg atagtcccgg tggagattat
     2281 ctcacccata cggatgccag tttgtattcc ccctttatcc aatggaacgg agagcgaaat
     2341 gtacgatgta gctttttgca gcaatgtgaa accgaatctg gatacgacgg atttcggctt
     2401 gaatataccc tggatggtgg cctgaactgg ctgccggtcg gtcagcccgg cgatgagaat
     2461 accgaaaact ggtacgatca tgccgctttg tctgcattcc aggcaggacc gggatggtct
     2521 ggactcctgc ctgaatggag gaacagtcgt tgtctgcttt cagcgctcga tggttgggtt
     2581 ggcctggtgc aattccggtt tcatttgtca acggatggaa gtaacgtggc ttcgggcgtg
     2641 cggatcgatg actttcgttt ggaacctgct ccgggaatag atttgtccat caatgacgtc
     2701 tccgtcagcc atcgtttcgc ggcggctgcg ttcccggatt ccatacacat ccggatccgg
     2761 aataccggct ccaccgattt gcaggatcct gtcgttcaac tcgaactcgg tacgttggtc
     2821 aggagcttcc agtattctgg cgtactgcaa ccgggcgctg ccgctgactg gacgatcgac
     2881 acgattggac ttccggaagg tccgtcggag ttgacgatac aatccgctgt gccctccgac
     2941 ggagacaacc tgaatgattc ccgttcactt cagctcttcg gtatcccgac ccgcaccttg
     3001 ccgttcacgg atgatttgga atctgccatc ttgtttgccg atacttccgc aaactggtcc
     3061 ctggggaaac cggctttttc cagcgagacc agtgcggctt ccggtatgaa tgcctggaac
     3121 ctgccgggaa gcggttccta cccggatcga gtgactgctg tcttgtacac accgtatttc
     3181 aatctgcggg gatggtacaa tcctcgtttg aagttcaatc attggttcaa tacccagagc
     3241 ggaactgacg gcggtcgggt agaggtttcg gtggatagtg gaatgacctg gtccacactc
     3301 ggtcaacgaa acgatccgct cgggatcaat tggtacaata ctgcggcatt ggcttccagc
     3361 ggacagtcgg gatggagcgg tggttccggt aattggagcg aaagcgtcta ttggatgccg
     3421 gcctattcga atgcccaggc gctcgtccaa ttccggttcg tctttcaatc ggatgactct
     3481 gttgcagtcg atggctggtc gatcgatgac attcgactcg aggcatcgcc ggcacattcc
     3541 gtttcaccgg aagcggtgaa cggtttgccg gatccgatca atcccgtatt gccggctgct
     3601 ctgcaatgcc gtgtccggaa ttcgggcgca ttgccggtaa gtgaatttga tcttcgcgtg
     3661 ttgatcgacg attcactctt ccatcaacaa cactgcacct tgcccacacc gatcggaaac
     3721 gggcaggtgg ctaccatcca gctttcatcg ccctggcaag cccctcccgg tggtcatgaa
     3781 gttacggtca ttgcatccgc tacctccccg gcagaagcct ggcctgccga tgataccttg
//
LOCUS       DAOTON010000172         3826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19122_length_3826_cov_3.164413, whole genome shotgun sequence.
ACCESSION   DAOTON010000172 DAOTON010000000
VERSION     DAOTON010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19122_length_3826_cov_3.164413"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="P5292_04565"
     CDS             complement(<1..296)
                     /locus_tag="P5292_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRS38433.1"
                     /translation="MNRTTILCAALLFLGSLAAVAQQKSDAIILNIAGEDIPKSEFER
                     VFKKNNTKDNNYDQKAVNDYLQLYINYKLKVREALELGMDTVKSFVDELAGYRK"
     gene            384..2183
                     /locus_tag="P5292_04570"
     CDS             384..2183
                     /locus_tag="P5292_04570"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRS38434.1"
                     /translation="MNSRFGDQSVNTWKEQINVMSKAEFLNYFTAPNPSPMNADLRRL
                     LTLLLLLPVLADAQSSNPMPIPPLLSGKNFSLSCDTSSVFFYPGMRTSTFGVNGSFLG
                     PTLEFLKWDTVSIDVMNNLQEMTTMHWHGMHVPGDMDGGPYQEIMPGMMWNATYRVLN
                     NAGTYWYHPHPHMMTQSQVTMGMAGFIIVRDSAEAALALPREYGVDDFPVVLQDRKWN
                     TVTGQFEVTALGDSMLINGVPNAYLDVPSRIVRLRVLNGSNARVYQLGFLDNRTFQVI
                     GNDGGLLAAPVTVSRLLLSNGERAEILLDLNGLQGDSLQLMSYATEMSTTIPGNMSAG
                     MGGTGPLERVDFPLLKLRVQGPGTGGVTSIPATLVPQYVWNPNDVNRIRNRTITGMGM
                     PGMGNFYLDGNTFSMNFVNDTMRLGDIEVWYMTNASNMAHPMHTHDVQFYILEKNGQA
                     PPPEESGAKDVVLIVPGDSITIITRFLDYADVTSPYMYHCHNLAHEDMGMMTSFVVID
                     TATTLVRDILDQGFAVYPNPSSTDWTLHMANPDQPLEWKLTDLQGAVIQQQRWTDTSG
                     KDLIIPGRNLAPGLYLLSIQSGTTTRVARLVRL"
     gene            complement(2193..3662)
                     /gene="guaB"
                     /locus_tag="P5292_04575"
     CDS             complement(2193..3662)
                     /gene="guaB"
                     /locus_tag="P5292_04575"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRS38435.1"
                     /translation="MKVATGRFLGEALTYDDVLLIPAYSEVLPNQVDVSSQLTPGIRL
                     NMPILSAAMDTVTEADMAVAIAHEGGIGMLHKNMTAEQQAAEVRRVKRSESGMIDDPV
                     TLFEDAKVHDALRIMRDYRIGGIPVIDHDRKLVGICTSRDLRFEKDVGRPIAQIMTKD
                     RLVTTTGVKDLKEAESILQRYKIEKLPVVDKKGRLVGLITYKDILKFKSRPNACKDEF
                     GRLRVGAAIGVTRDSLERADALVKAGVDIITIDTAHGHTKGVVEVLKAVKKKHPKLPV
                     IVGNIATADAARYLAKAGADAVKVGIGPGSICTTRVIAGIGVPQFTAVYQVAEALKGS
                     KVGVIADGGIRYSGDIAKAIAAGAHCVMLGSLLAGTEESPGETIIYEGRKFKTYRGMG
                     SIEAMREGSKDRYFQAQTDDVKKLVPEGIVGRVPYKGTVGEELYQLVGGLRASMGYCG
                     APNIASLQKASFIRITSAGVRESHPHDVSIVKDAPNYHR"
BASE COUNT          848 a         1045 c         1013 g          920 t
ORIGIN      
        1 tgtttccggt aaccggccag ttcatctacg aacgacttga ccgtatccat gccaagttcg
       61 agcgcttcac ggaccttcag cttgtaattg atatacaact gcaggtaatc gttgaccgct
      121 ttctggtcat agttattgtc cttggtgttg ttcttcttga aaacacgctc gaactcactc
      181 ttgggaatat cctccccggc gatgttgaga ataatggcat ccgacttctg ctgcgctacg
      241 gcggcaaggc tgcccaggaa cagcagggct gcacacagga tggttgtacg gttcattttg
      301 gttaaacaat tgagcggcgc aaatttagca atccataccg ggtaccaaaa gatgaagaaa
      361 ggtcattttc agatgaattt cacatgaact cgagatttgg tgatcaatcg gtgaatacct
      421 ggaaagagca gataaatgtc atgtcaaaag cggaattcct gaattacttt accgccccta
      481 atccatcacc catgaatgct gacctgcgcc ggcttttaac gctcctccta ctactacccg
      541 tgctggcgga cgctcaatcg tccaatccga tgccgattcc accgctactc agcggaaaga
      601 atttcagtct cagttgcgat acttcctccg tcttcttcta tccgggaatg cggaccagca
      661 cctttggggt aaacggatca tttctgggtc cgaccctgga atttctgaag tgggataccg
      721 tttcgatcga tgtcatgaac aaccttcagg agatgaccac gatgcactgg cacggcatgc
      781 acgtgccggg tgacatggac ggagggccct atcaggagat tatgccgggt atgatgtgga
      841 acgcgaccta tcgtgtgctc aacaatgccg gaacctattg gtatcatccg catccgcaca
      901 tgatgaccca gtcgcaggtc accatgggca tggccgggtt catcatcgta cgggattccg
      961 cggaagccgc gcttgcattg ccgcgtgaat atggtgtcga tgattttccg gtcgtattgc
     1021 aggatcgtaa atggaatact gtaaccggac aatttgaggt taccgcactg ggtgactcca
     1081 tgctgatcaa cggagtaccg aatgcttatc tggatgtgcc gagccggatc gttcggctgc
     1141 gcgtactcaa cggatccaat gcccgcgtct accagcttgg gttcctggac aaccgaacct
     1201 ttcaggtcat tggcaacgat ggaggtttgc tcgctgcacc ggtgaccgtc agccgactgc
     1261 tgctttccaa cggcgaacgc gcggagatcc tgctcgatct gaacggtctc cagggcgact
     1321 ccctgcaatt gatgagctat gcgacggaaa tgtcgaccac cattcccggc aacatgagcg
     1381 cgggcatggg gggtaccggc ccgctcgagc gtgtcgattt cccgctcttg aaactgcgcg
     1441 tccagggacc cggaaccggc ggagtgacca gcattcccgc taccttggtt ccacaatacg
     1501 tttggaatcc gaatgatgtc aaccgtatcc gcaaccggac tatcacgggg atgggcatgc
     1561 cgggcatggg caatttctac ctcgacggga atacgttctc gatgaatttt gtcaacgata
     1621 cgatgcgcct gggcgatatt gaagtgtggt acatgaccaa cgcctcaaac atggcgcacc
     1681 ccatgcatac gcacgatgtg cagttctaca tcctcgaaaa gaacgggcag gcaccgccac
     1741 ccgaagagag cggagcgaag gatgtggtcc tgatcgttcc gggcgattcc atcaccatca
     1801 tcacgcggtt tctggattat gccgatgtga cctcacccta catgtaccat tgtcacaatc
     1861 ttgcccacga ggatatgggg atgatgacct cgtttgtggt tatcgatact gccaccacac
     1921 tggttcgcga tatcctggat cagggcttcg ctgtttaccc gaatccgagt tcaacggact
     1981 ggacgctgca catggccaat ccggatcaac cgctggagtg gaaactgacc gaccttcaag
     2041 gtgctgtcat ccaacagcag cgatggacgg atacgtctgg aaaagacctg atcatcccgg
     2101 gcagaaatct ggcacccgga ctttatctct taagcattca atccggaacg accacccgtg
     2161 tagccaggct ggtcaggttg tagcagagcc gttcagcggt ggtaattcgg agcatccttc
     2221 acgatggaaa catcgtgtgg atggctttcg cgaacgccgg cgctggtaat acggatgaag
     2281 ctggcctttt gaagcgacgc gatgttgggt gctccgcagt atcccatgga agcccggagg
     2341 ccgcccacca actggtacaa ctcctcgcct accgttcctt tgtaaggtac gcggccgacg
     2401 attccttccg gtaccagttt cttcacatcg tccgtttgtg cctggaaata acggtccttg
     2461 gaaccttcgc gcatggcttc gatggaaccc atcccgcgat aggtcttgaa ctttcttcct
     2521 tcgtaaatga tcgtttcgcc cggagactct tcggtgccag ccaacaggct gccgagcatt
     2581 acgcaatgcg ctccggcagc gatggctttc gcgatgtctc cgctgtatcg gataccgcca
     2641 tcggcaatga cacccacctt gcttcccttc aaggcttccg cgacctgata gacagcggta
     2701 aactgcggta cgccgatccc ggcgatgacg cgcgtcgtgc aaatcgatcc gggaccgatc
     2761 ccgaccttga ctgcatccgc gccggctttc gcgaggtatc gtgcggcatc cgccgttgcg
     2821 atgtttccga cgataaccgg gagtttaggg tgtttctttt taacggcttt gaggacttct
     2881 accacgccct tcgtatgtcc gtgtgccgtg tcgatcgtga tgatgtcgac accggctttc
     2941 accagcgcat cggcacgctc cagactgtcg cgcgtcacgc cgatcgctgc tccgacacgc
     3001 aagcggccaa actcgtcctt gcaagcattg gggcgcgatt tgaacttgag tatgtcctta
     3061 tacgtgatca aaccgacgag acgacctttc ttatcgacta ccggtaactt ctcaatcttg
     3121 tatcgctgca ggatgctttc cgcttctttc aggtccttga cgccggtggt ggttaccagc
     3181 ctgtccttgg tcatgatctg ggcaatcggg cgacctacat ctttttcaaa ccggaggtca
     3241 cggctggtgc agataccgac caatttccga tcgtggtcaa tgaccgggat acccccgata
     3301 cggtaatcgc gcatgatccg cagggcgtcg tgcactttcg catcctcaaa aagtgtgacc
     3361 ggatcgtcga tcattccact ttcactgcgc tttacccgtc gaacctcagc ggcctgctgt
     3421 tcagccgtca tattcttgtg caacatcccg atgccacctt cgtgggcgat ggcaaccgcc
     3481 atatcggctt cggtgaccgt gtccatagcc gcggatagaa taggcatatt caaccggatg
     3541 ccgggagtca actggcttga tacatctact tgatttggaa ggacttccga gtaggccggg
     3601 atcagaagta catcatcgta ggtgagcgct tcacctaaaa atcggccggt tgcaactttc
     3661 atagacggag tctgggttga aaaaaagttg cacgcaaagg tagggattta tggggagatg
     3721 tggtggggga atttttggaa tagtgaatag tgaacagtga acagtaaaca gtgaatagtg
     3781 aatagcgaat agcgaatagt tattagttac tagttaccag tttcat
//
LOCUS       DAOTON010000173         3810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19219_length_3810_cov_3.146471, whole genome shotgun sequence.
ACCESSION   DAOTON010000173 DAOTON010000000
VERSION     DAOTON010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19219_length_3810_cov_3.146471"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /gene="folP"
                     /locus_tag="P5292_04580"
     CDS             <1..754
                     /gene="folP"
                     /locus_tag="P5292_04580"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRS38436.1"
                     /translation="HLASRVSEQEAILDLVGRHLREGAAILDLGAQSTRPGADLLDAE
                     TEWSRLSPGLELIRGNFPDAILSIDTFYASVAERAVAKGADMINDVSGGQLDAAMFST
                     IGRLKVPYVLMHMQGTPKSMQRSPEYRDVTAEVIRHLAEGIDRLRQSGVPDVIVDPGF
                     GFGKDLSHNYTLLRNLDAFRVLDAPLLVGLSRKSMVNKVLGTTAEQALNGTTVLNTLA
                     LTGGAKILRVHDVREAVEAIRLFERYCSPAGA"
     gene            826..1629
                     /gene="cdaA"
                     /locus_tag="P5292_04585"
     CDS             826..1629
                     /gene="cdaA"
                     /locus_tag="P5292_04585"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRS38437.1"
                     /translation="MDLFKIGFLTVRLIDLIDIGIVTFLLYKLYHLLRGGVAINIFIG
                     LLTVYALYWICVKVLNMELLGTILGQFISLGFIALIIVFQQEIRRFLVVLGTNNILAK
                     SPFTRQLLPWNWQTQRTPLDISAIVKACRQMSKDRTGAIIVLAKSSELKYYANTGDLI
                     DAEISQRLIESVFFKNSPLHDGAIIVVNNKIKAARCVLPVTENVELPAHLGMRHRAAI
                     GISEHSDALALVVSEETGEISIAKEGQIRINVSLEELEKAMLEDFGAET"
     gene            complement(1632..2168)
                     /locus_tag="P5292_04590"
     CDS             complement(1632..2168)
                     /locus_tag="P5292_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS38438.1"
                     /translation="MRGYSLLISFCFLLLQACTSPQQKLRDAIAAGEQRLINDSTRLP
                     DLAMAEKVSHLYVQYANEYKEDTLSAEYLFRAGDLANGMRHFDDAVTYYRRLRTEYPQ
                     HRKVAAALFMEAFNLQTGLNRNEEARTCYLEFLAKYPSHPMAEAARLSVDQINSGLSD
                     EELVRMFEAKNDSLAAGK"
     gene            complement(2177..2731)
                     /gene="def"
                     /locus_tag="P5292_04595"
     CDS             complement(2177..2731)
                     /gene="def"
                     /locus_tag="P5292_04595"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRS38439.1"
                     /translation="MILPIYAYGDPVLKKKASPIEKDHPGLKELIANMYETMYASHGV
                     GLAAPQIGLSLRLFVTDGSPFEEDEVKDFKQVFINPQIVEEAGDPWKFNEGCLSIPHV
                     REDVSRKETVLIEYYDEDWNFRRELFSGLAARIIQHEYDHIEGVLFTDRISPLRRKLL
                     NKRLSDISSGNVKVDYKMRFPSQR"
     gene            complement(2735..3157)
                     /gene="ruvX"
                     /locus_tag="P5292_04600"
     CDS             complement(2735..3157)
                     /gene="ruvX"
                     /locus_tag="P5292_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRS38440.1"
                     /translation="MGRIIGIDYGTRRVGLAVTDPLQLIATDLATVAEQETINWLKDY
                     LKRETVDRFVVGEPRQTDGSPSETGPAADAFAQRLARSFPDIPIDRFDERFTSRMAQQ
                     AIIQSGVRKKGRQDKGLVDRVSAVILLQSWMEARNFKA"
     gene            3226..>3810
                     /locus_tag="P5292_04605"
     CDS             3226..>3810
                     /locus_tag="P5292_04605"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008866516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRS38441.1"
                     /translation="MHELIEKAWENRSLLQDPATQEAIRTVIGHLDKGILRVAEPQGD
                     SWIVHDWVKKAVILYFPIQKMETWNAGPMEFHDKMLLKRYYAELGVRVVPHGVARYGS
                     FLAHGVILMPSYVNIGAYVDEGTMVDTWATVGSCAQVGKHVHLSGGVGLGGVLEPVQA
                     APVIIEDNCFIGSRCIVVEGVRVGKEAVRGANVVL"
BASE COUNT          851 a         1080 c         1015 g          864 t
ORIGIN      
        1 ccaccttgcc agccgggtaa gtgaacagga agcgatcctg gacttggtcg ggcggcacct
       61 acgagaaggg gcggccatcc tggaccttgg cgcgcaatct acccgaccgg gagccgacct
      121 gctcgatgcc gagaccgagt ggtcccgcct ctcccctggt ctggaactga ttcgcggaaa
      181 tttccccgat gcgatcctgt ccatcgatac cttttacgct tcagtcgcgg agcgcgccgt
      241 tgctaaggga gccgatatga tcaacgatgt ttccggaggc caactggacg cggccatgtt
      301 ctccaccata ggccgcctta aggttcccta tgtgctcatg cacatgcaag gcacaccgaa
      361 gtcgatgcaa cgatcacccg aataccggga tgttacagca gaagtcatcc gccaccttgc
      421 agaaggcatc gacaggctcc ggcaatcggg agttccggat gtgatcgttg acccgggttt
      481 cggtttcggc aaagacctct cgcataatta caccttgctt cggaatctgg acgcgtttcg
      541 cgtgctggac gcgcctttac tggtggggct atcccgaaaa agtatggtaa acaaggtcct
      601 gggcaccact gcggagcagg cgctgaacgg cacgactgtt ctgaatacgc tggcattgac
      661 cggaggcgcg aagatcctgc gggtgcacga tgtccgggaa gctgttgaag cgatccgtct
      721 attcgagcgc tactgttcac cggctggcgc ctgaaccgaa cggttcataa cacttccggt
      781 agtcgaattc cgattccgta ataattccga aattcgttca gcgccatgga ccttttcaag
      841 atcggctttc tgaccgtccg cctgatcgac ctgatcgata tcgggatcgt gactttttta
      901 ctgtacaaac tctaccatct gctgcgcggc ggtgtcgcca tcaacatctt catcggattg
      961 ctgacggtat atgccttgta ctggatctgt gtgaaggtgc tcaacatgga actgctggga
     1021 acgatcctgg gacaattcat cagcctcgga ttcatcgcgc tgatcatcgt cttccaacag
     1081 gagatacgcc gcttcctcgt cgtcctcggc acgaataaca tcctcgccaa gtcgcctttt
     1141 acccggcaac tgctgccctg gaattggcaa acccaacgga cgcccctcga catttccgcg
     1201 atcgtcaagg cctgcaggca gatgtcgaaa gacaggaccg gcgccatcat cgtgttggcg
     1261 aagtcctccg aactcaagta ctatgcgaac accggcgacc tcatcgacgc ggagatatcc
     1321 caacgcctga ttgagagcgt gtttttcaaa aactcccccc tgcacgacgg cgccattatc
     1381 gtggtcaaca acaagatcaa agccgcccga tgcgtattac cggtcacgga aaacgttgaa
     1441 ctaccggctc acctcggaat gcgacatcgc gcggcgatcg gcatctcgga gcatagcgat
     1501 gcgctcgcgc tggtcgtctc tgaagaaacc ggggaaatct cgatagccaa agaaggtcag
     1561 atccgcatca acgtgagcct ggaggaactc gaaaaagcga tgctggaaga tttcggagcc
     1621 gagacctgaa cttatttccc tgccgccagc gagtcgtttt tcgcctcgaa catgcgcacg
     1681 agctcttcat ccgacaagcc ggaattgatc tgatctactg acaagcgggc tgcttcggcc
     1741 atcggatgcg acggatactt cgccaggaac tccaggtagc aggtccgcgc ttcttcatta
     1801 cggttcaagc ccgtctgtaa attgaacgcc tccatgaaca aggcggcagc gaccttccgg
     1861 tgctgcggat attcggtgcg aagtcgccga tagtaggtca ccgcatcatc gaagtgacgc
     1921 ataccgtttg caagatctcc agcccggaaa aggtattccg ccgaaagggt atcttcctta
     1981 tactcgttcg cgtattgaac atacagatgc gacacctttt cggccatggc cagatccggc
     2041 aaacgtgtcg aatcgttgat cagtctttgc tcacctgccg caattgcgtc ccgtaacttt
     2101 tgctggggtg acgtacaagc ctgcagcagc aggaagcaga aagaaatcaa gagagaatac
     2161 ccgcgcattt tttcaattat cgttgagaag ggaaacgcat cttgtaatcg actttgacgt
     2221 taccgctact gatgtcgctt aagcgcttat tcaacaactt gcgtcgaagc ggtgaaatcc
     2281 ggtcggtaaa cagcacccct tcaatgtggt cgtattcgtg ttggatgatc cgggcggcaa
     2341 ggccggagaa gagttccctc cgaaaattcc agtcttcgtc gtaatattcg atcaacacgg
     2401 tctcctttcg gctgacatcc tcccgaacgt gtggaatact caggcaaccc tcattgaatt
     2461 tccagggatc acccgcctcc tcaacgatct gcggattgat gaaaacctgc ttaaaatcct
     2521 ttacctcatc ctcctcaaac ggtgacccat cggttacaaa caggcgcagg gaaaggccga
     2581 tctgaggcgc tgccaaaccg acgccatggg atgcgtacat cgtttcgtac atattggcga
     2641 ttaactcttt caggccggga tggtcttttt cgatggggct cgccttcttc ttcaatacag
     2701 gatcgccgta agcgtaaatg ggtagtatca tgtctcaggc cttgaaatta cgggcttcca
     2761 tccacgactg caacaagatg acagcactta cccggtcgac caacccctta tcctgtcggc
     2821 ccttcttgcg tacaccactt tgaataattg cctgttgtgc catccgggaa gtaaaccgtt
     2881 catcgaagcg gtcgatcggt atgtcgggaa atgatcttgc caatctttgt gcgaatgcat
     2941 cagccgccgg tcctgtttcc gaagggctcc catccgtttg acggggctcc ccgaccacga
     3001 accggtcaac cgtttcccgc ttcaggtaat ccttcaacca gttaatcgtt tcctgttcgg
     3061 cgaccgtggc aaggtcagtt gcaatgagtt gcagagggtc ggtgaccgcc agtccgactc
     3121 gacgtgttcc gtaatcgatg ccaatgatcc gccccattcg gcaaagatag ccctttgccg
     3181 tatgtttgag gccgggaatc ggcctattgc cattccaagc tcggtatgca cgaactcatc
     3241 gaaaaggcct gggaaaaccg ctcgctctta caggatcctg ccacgcagga agcgatccgg
     3301 accgttatcg gtcacctgga taaagggata ctgcgtgtcg cagaaccgca gggcgacagt
     3361 tggatcgtac acgactgggt gaaaaaagcc gtcatcctct acttcccgat ccagaaaatg
     3421 gaaacctgga atgccgggcc gatggagttc cacgacaaga tgttgctcaa acgctactac
     3481 gcggagcttg gagtccgtgt cgttccgcac ggtgtcgccc gttacggtag cttcctggca
     3541 catggcgtca ttctaatgcc gtcttacgtc aacatcgggg cttacgtgga cgaaggtacc
     3601 atggtggata cctgggcgac tgtcggctcc tgtgctcagg ttgggaaaca cgtgcacctg
     3661 agtggaggcg tcgggttggg tggcgttctt gagccagtgc aagccgctcc ggtgatcatc
     3721 gaagacaatt gcttcatcgg atcacggtgc atcgtagtgg aaggtgtccg cgtcggcaag
     3781 gaagctgtcc gcggtgccaa cgtggtactg
//
LOCUS       DAOTON010000174         3806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19240_length_3806_cov_2.748067, whole genome shotgun sequence.
ACCESSION   DAOTON010000174 DAOTON010000000
VERSION     DAOTON010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19240_length_3806_cov_2.748067"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..2917
                     /locus_tag="P5292_04610"
     CDS             146..2917
                     /locus_tag="P5292_04610"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRS38442.1"
                     /translation="MCLLCLAAAGQQYDLQTIIPVDPAVRTGVLSNGLTYYIRKNSKP
                     ENRAELRLAVNAGSTAEDDGQRGLAHVVEHMAFNGTKNFKKSELVDYLESIGTKFGPH
                     LNAYTSFDETVYMIQLPTDKPEILDKGLQIMEDWSHNLLFDSLEVEKERGVVVEEWRL
                     GQGADERMRRAYWPVLFKDSRYAERLPIGKKEIIEGCPQSALRNFYNDWYRPELMAVI
                     LVGDFDLDAMEAKVKARFSNVPSRQNPRPLVKWPVPAQNNLAVSTVTDKEARYAQIQL
                     IYKRPVESRSTEGDYRRSLAQQLFTGMLNNRMGELQRLAEPPFLFAGSDYGNFVRNVN
                     AYYSFAISKGEGIEKALQTVLDENERVRRFGFTESELSRQKSDMMTGIEKAFNERDKT
                     ESRNFAREYVSNYLEDEPMPGISFEYDLYKKYVPGITLAEVNAFAKEWMGQSQNAVVI
                     ITAPEKETTKMPTETRIREMVAGMEKLDLKPYEDKVIDKPLVEGQLKGGTVVNTKEIK
                     ELGITEWQLDNGIKVACKTTDFKNDEVIFTSFSWGGWSQYDEKDYPSAASCDEIVDQA
                     GLGAFDANALEKLLAGKIVSCSPYVGELTEGFNGSCAPKDMETMFQLIYKYFTDPRRD
                     EAAFQSYVTKQKGFLQNRGSDPQSVFMDTVSYAMSGYNARYKPRTPESVDAINLDRAM
                     AIYKERFADASGFTFFFVGNFKPEELKAHCQKYLGSIPATRKREMWQDINAYPPKGKV
                     EKTVYKGSEPRSTVMMRFNTDFEFNRKNRNEVSALNKLLSIRLREVLREDKSGVYGVS
                     CQANPKKFPANGLEFIVYFSCSPDNVEPLTKAALDVFEEVKTKGCDEKNLTKIKETAL
                     RERETNLKENQFWLSLMSSSAQNGEEVTEILGYNEWVNKLSVADMNAFAAKYLKLDNY
                     ARFVLRPEK"
     gene            complement(3020..>3806)
                     /gene="miaA"
                     /locus_tag="P5292_04615"
     CDS             complement(3020..>3806)
                     /gene="miaA"
                     /locus_tag="P5292_04615"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRS38443.1"
                     /translation="GTAKPTAAELKSVPHHFIDFLSVTEDYNIGKFEQDALKRLDELF
                     LKHRIVILVGGSGLYIRAVCQGLDELPPGDPIIREEVIRLWQEQGLPALQAELESADP
                     AYYQTVDLKNPSRLIRALEVCRQTGLPFSTFRQSKAKPRNFSIATLILNPERTALYAR
                     IDQRVDEMMQHGLLEEVRGLLPYKDRNPLATVGYQELFPYFEGSSSLESAVALIRQHS
                     RNYAKRQMTWFRKVPNATWIHPSDRASIVQWADTVTQSFRNAS"
BASE COUNT          951 a          929 c         1071 g          855 t
ORIGIN      
        1 agcagcttta ttttcatttt gccacggatt taaatggtgt cctgcaacgt ttgtagaact
       61 gattattaaa gatatgaatt cgcaatgaac cgattagctt agtgcaaaat ttaaacggat
      121 gcggtttttt caaactttgt tattggtgtg cctgctgtgc ctcgctgctg ccggccagca
      181 atacgatctc caaacaataa ttccggttga tccggccgtc agaacaggcg tgctctccaa
      241 tggcctcact tattatatcc ggaagaacag taagccggag aaccgggcgg aattgcggct
      301 tgcggtcaat gcgggctcaa cggccgagga cgatggtcaa cgcggccttg ctcacgtcgt
      361 ggagcatatg gcgtttaacg ggaccaagaa cttcaagaaa agcgagttgg tcgactacct
      421 ggaatccatc ggaacgaagt tcggtccgca cctgaatgcg tacacgagct ttgatgagac
      481 ggtttacatg atacagttgc cgacggacaa gcctgaaatc ctcgacaaag gactgcagat
      541 catggaagac tggtcgcata acctcttatt cgattcgctg gaggtggaaa aggagcgtgg
      601 cgttgtcgtg gaagaatggc gactcggtca gggagccgac gaacggatgc gccgggccta
      661 ctggccggta ctttttaagg attcccgtta cgcggagcgt ttacccattg gtaagaagga
      721 gatcattgaa ggttgtccgc aatccgcttt gcgaaacttc tacaacgact ggtaccgtcc
      781 cgaattgatg gccgtgatat tggtaggtga tttcgacctc gatgcaatgg aagcgaaagt
      841 gaaagcgcgt ttcagcaacg ttccttccag gcagaatccc cggcccctgg tcaagtggcc
      901 ggtgccggca cagaataacc tggccgtaag tacggtcacg gacaaggagg cgcgttatgc
      961 ccagatccag ttgatctaca agcgacccgt cgaatcccgt tctacggaag gcgattatcg
     1021 ccgttcactc gcgcagcaat tgtttaccgg tatgttgaac aaccggatgg gcgaattgca
     1081 gcgattggca gagccgcctt tcctgtttgc cggttctgat tacgggaatt ttgttcggaa
     1141 tgtgaatgct tactactcct ttgccatcag caaaggcgag ggaattgaga aggcgcttca
     1201 aaccgtgttg gatgagaacg agcgggtacg tcgtttcggc tttacggagt cagaattgtc
     1261 ccgccagaaa tccgatatga tgaccgggat cgaaaaggct ttcaacgaac gggacaagac
     1321 cgaatcaagg aatttcgccc gtgagtatgt cagcaattat ctggaagacg agccgatgcc
     1381 cggaatctcc tttgagtatg acttatacaa gaagtatgtt cccggaatca cgctggcgga
     1441 ggtaaatgca tttgccaaag agtggatggg acagtcacaa aatgcggtgg tgatcattac
     1501 tgcaccggaa aaggaaacga ccaagatgcc gacggaaacg cgcattcgcg agatggttgc
     1561 cggcatggag aagctggatc tgaaacccta cgaggataag gtgattgaca aaccgctggt
     1621 cgaaggacaa ctgaaaggcg gaacggtcgt gaacacgaag gagatcaagg aactcggcat
     1681 taccgaatgg caattggaca atggcatcaa ggtcgcgtgc aagaccaccg atttcaagaa
     1741 cgatgaagtg atctttacgt cctttagctg gggcggctgg tcacaatacg atgagaaaga
     1801 ctatccctct gctgcttctt gcgacgagat cgtggaccag gcaggattgg gcgcatttga
     1861 cgcgaatgcc ctggaaaagc tcctggcagg gaagatcgtt tcttgctcgc catacgttgg
     1921 ggaattgacg gaaggattca acggtagttg tgcgcccaaa gacatggaaa ccatgttcca
     1981 gttgatctac aagtacttca ccgatccccg ccgggacgaa gcggccttcc agtcgtacgt
     2041 caccaagcag aagggattcc tgcaaaaccg cggttccgac ccacagtcgg tcttcatgga
     2101 tacggtctcc tatgccatgt cgggttacaa tgcccgctac aagccgcgta ctccggagtc
     2161 cgtcgatgcc atcaacctcg atcgtgcaat ggccatctac aaagagcgtt ttgcggatgc
     2221 atccggcttc acctttttct tcgtcggcaa cttcaagccg gaagaactga aagcccattg
     2281 ccagaagtat ctgggaagca taccggctac tcgtaagcgc gaaatgtggc aggatatcaa
     2341 cgcgtatcct ccaaagggaa aagtcgaaaa gacggtctat aaaggctccg agccgcgcag
     2401 tacggtcatg atgcgcttca atacggattt tgagttcaat cgaaagaacc ggaatgaagt
     2461 cagcgcattg aacaaactcc tcagcatccg gttgcgcgag gtgctgcgtg aagacaagag
     2521 cggtgtatac ggcgtgagct gtcaggctaa cccgaaaaag ttcccggcca acggtctgga
     2581 gttcatcgtc tatttctcct gcagtccgga taacgtcgag ccactgacca aagccgcact
     2641 ggacgtgttc gaagaagtga agacaaaagg gtgcgatgag aagaatctga ccaagatcaa
     2701 ggagaccgcg ttgcgtgagc gcgaaacgaa cctgaaggag aaccagttct ggcttagcct
     2761 gatgagttca agtgcacaga acggtgagga agtcaccgag atccttggtt acaacgagtg
     2821 ggtgaacaaa ctcagcgttg ccgacatgaa tgcatttgct gcgaagtacc tgaagctgga
     2881 caactacgcc aggtttgtcc ttcgacccga gaagtaatcg ggaacctgct gacactaaag
     2941 ggagctgaaa ggctcccttt tttgggtctc gaaattgagt aggggattga cccttctcgg
     3001 tcgcgcttca acgccgtgat caggacgcgt tccggaagga ctgtgtcacc gtatctgccc
     3061 actgcacgat gctcgccctg tccgagggat gtatccaggt tgcgttgggt acttttcgga
     3121 accaggtcat ttgtcgtttc gcgtagttcc gactgtgttg tctgatcagc gcaacggccg
     3181 attcgagcga agaacttccc tcgaagtagg ggaagagttc ctgatagccg acggtggcca
     3241 ggggatttcg gtccttgtag gggagtaacc cgcgcacttc ttccagtaaa ccatgctgca
     3301 tcatctcatc gacccgttgg tcaatgcgtg cgtacagagc cgttcgttca gggttcagga
     3361 tgagtgtagc gatggagaaa ttcctcggtt tggcttttga ttgccgaaaa gtggaaaacg
     3421 gcaggccggt ttgacgacag acttccaaag cccggatcaa ccggctggga ttcttgagat
     3481 ctaccgtctg gtaatatgca ggatcagcgc tttccaactc ggcctgcagt gccggaagtc
     3541 cctgttcctg ccagaggcgg atgacttctt cccggataat gggatcgcca ggcggcaatt
     3601 cgtccagacc ctggcaaact gcccgtatat acagccccga tccgccaacc agtatcacga
     3661 tgcggtgttt aagaaacaac tcatccaggc gcttcaaggc atcctgctcg aatttaccga
     3721 tgttgtagtc ttcggtaacc gataagaagt ctatgaaatg gtgggggacg gatttcagct
     3781 ctgccgctgt aggcttggcg gtcccg
//
LOCUS       DAOTON010000175         3800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19255_length_3800_cov_3.476368, whole genome shotgun sequence.
ACCESSION   DAOTON010000175 DAOTON010000000
VERSION     DAOTON010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19255_length_3800_cov_3.476368"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="P5292_04620"
     CDS             complement(<1..338)
                     /locus_tag="P5292_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRS38444.1"
                     /translation="MPVNQQEIQRFGHLELLAKQVVEGFITGLHKSPFHGFSVEFAEH
                     RLYNTGESTRHIDWKLFGRTEKLFVKRYEEETNLRCQLVIDNSSSMHFPEPGQSKIPN
                     KIDFSVHAAAA"
     gene            complement(434..754)
                     /gene="trxA"
                     /locus_tag="P5292_04625"
     CDS             complement(434..754)
                     /gene="trxA"
                     /locus_tag="P5292_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRS38445.1"
                     /translation="MALEINDQNFEELVMKSDKPVLVDFWAEWCGPCRMVGPIVEELA
                     KEYDGKAVIGKVNVDHNPNISMNFGIRNIPTLLIFKNGQVVDKQVGAVPKSILANKLN
                     AQIA"
     gene            complement(848..>3800)
                     /gene="dnaE"
                     /locus_tag="P5292_04630"
     CDS             complement(848..>3800)
                     /gene="dnaE"
                     /locus_tag="P5292_04630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRS38446.1"
                     /translation="DSHYTDRDDYNAHDILLCINTGEKQATPTMKEFSDDDAFTKGKR
                     FAFYNDQFYFKTTDEMSALFADVPEAIDNTNEIVSKVDTLRLKQDILLPHYQLPKGFT
                     DQDEYLRHITFEGAKKRYGDIAPETEERLNFELFTIKTMGFAGYFLIVADFIKAGRDL
                     GVFVGPGRGSAAGSAVAYCIGITNIDPIKYNLLFERFLNPDRKSMPDIDTDFDDVGRQ
                     KVIDYVVTKYGRNQVAQIVTYGTMAAKMSIKDVARVLDLPLPESNALAKLVPERPGIE
                     LQRVLEAPLDGEKSLREKENLSSDDLEQVKRLREIYKGKDLQAAVLREALRLEGSVRN
                     TGIHAAGIIIAPKDLTEIIPVATSKDTELLITQYDGKVIEDAGVIKMDFLGLKTLTII
                     RDALRLIEQNHGVKIDIDAIPLDDTKTFELYQRGETNGTFQFESAGMQKYLKELRPDK
                     FEDLIAMNALYRPGPLEYIPNFIARKHGREAVAYDLPAMEEYLQESYGITVYQEQVML
                     LSQKLAGFTKGDADTLRKAMGKKQKDVLDKMKSKFMDGCKANGHDPEVCEKIWTDWEA
                     FASYAFNKSHSTCYAFVAFQTAYLKAHYPAEYMASVLTHNLGTIDKITFFMEECRRMG
                     IPVLGPDVNESLYDFSVNQQGQIRFGLGAVKGVGENAVISIIDERTKEGPYESIFDLT
                     KRVSSKAMNKKCLESLVQAGALDSLGSIERSRYFAIDPKDNANGIEKAIRFGSALQNK
                     QGSMQNSLFGDDHEETIALPVIPEGEFWNNLEKLRKEKEVIGFYISGHPLDEYRFEIR
                     SFCSHRIEQLANLEELKNREVSIAGILTEVNHRISKNGKPFGSFTVEDYTGGVQMVLF
                     GEDYMKMKDYLAQESFVFIRAKVQNRFNAPDQYEVRPYMMQYLSDVSDKMAKSFTLKL
                     PLSVLSEGLIDRLSDVLSASPGNCQVKIQVSDPVDNLAVDLPSRKLRIRLNRQLLEDL
                     DAIGTVEYQVN"
BASE COUNT          775 a         1023 c         1015 g          987 t
ORIGIN      
        1 gcagcggcgg catgcaccga gaaatcgatc ttgttcggga tcttcgattg cccgggttcc
       61 gggaagtgca tcgaggaaga attgtcgatg accaactgac aacgcaggtt ggtctcttct
      121 tcgtagcgtt tgacgaagag cttttcggta cggccgaaga gtttccaatc gatgtgccgg
      181 gtcgattcgc cggtattgta gagccggtgc tcggcgaatt ccacggagaa accatgaaac
      241 ggacttttgt gaagcccggt gatgaatccc tccactacct gcttggcaag cagttcgagg
      301 tgaccgaaac gttggatctc ctgttgattg accggcatgg gatcaaagat aacgaatcgc
      361 ttcgggcggt attgaaaagc aaagagcggc cagccttggg gctaaccgct ctgctctatg
      421 cgtccaggat cgatcaggcg atctgggcgt tcaatttatt ggccagaatc gacttcggta
      481 cggcgccgac ctgcttgtcg actacctgac cgttcttgaa gatcaacagg gtcgggatat
      541 tccggatacc gaagttcatc gaaatgttcg ggttatgatc cacgtttacc ttaccgatca
      601 cagccttgcc gtcgtattct ttggcgagtt cttccacgat cggacctacc atgcggcagg
      661 gtccacacca ctcggcccaa aaatcgacga ggaccggctt atcggacttc atgaccagtt
      721 cttcgaaatt ctggtcgtta atttctaatg ccatttttct ttggtttttt gatgttttta
      781 cttcttcccg tagcgggcac aaacttaggc aaaatcccag ccaaggccgg tttccgcctt
      841 attgtcacta attaacttga tattctactg tcccgatggc gtcaagatcc tccagaagct
      901 gcctattcag gcggattcta agctttctgg aaggtaaatc caccgccaga ttgtccaccg
      961 ggtcgctgac ctggatcttc acctggcaat tgcccggact ggcggacagg acgtccgaga
     1021 ggcggtcaat caacccttcc gacaagacac tcaaaggcag tttgagtgta aaggattttg
     1081 ccattttatc gcttacgtcg gacaggtact gcatcatata cgggcgaact tcatactgat
     1141 cgggtgcatt gaaccggttc tggactttgg ccctgatgaa gacgaatgat tcctgtgcca
     1201 ggtagtcttt catcttcatg tagtcctctc cgaacaagac catttgaacc ccgccggtat
     1261 aatcctcaac agtgaacgag ccgaatggtt ttccattctt gctgatacgg tgattcactt
     1321 ccgtcagaat tcccgcaatg gaaacttccc ggttcttcaa ctcctccagg ttcgccagtt
     1381 gttcgatgcg atgactgcaa aagctgcgga tctcgaaccg gtattcgtcg aggggatggc
     1441 cgctgatgta gaatccgatt acttcttttt ccttccgcaa cttctccagg ttattccaaa
     1501 actccccttc cggaatcacg ggcaatgcga tggtctcttc atgatcgtct ccgaaaaggc
     1561 tattctgcat ggaaccttgt ttgttctgca aggcgctacc gaaacgtatc gccttctcga
     1621 tgccgttggc gttgtctttg ggatcgatcg cgaaatagcg tgatcgctcg atcgatccaa
     1681 ggctgtccag cgcaccggcc tgcacaaggc tctccaggca tttcttgttc atcgccttgc
     1741 tgctgacacg tttggtaaga tcgaaaatgc tttcgtaggg cccctctttg gttcgttcgt
     1801 caatgatgga gatcaccgcg ttctccccaa cccctttcac cgcacccaat ccgaatcgaa
     1861 tctgtccttg ttggttgaca ctgaaatcat agagcgattc gttcacgtcg ggcccgagaa
     1921 ccggaatacc catccgccgg cattcttcca tgaagaaggt gatcttatcg atcgttccga
     1981 ggttgtgcgt cagtaccgaa gccatgtatt cggccgggta atgcgctttg aggtaggccg
     2041 tttggaacgc tacgaacgcg tagcaggtgg agtgtgattt attgaacgca taggaagcga
     2101 acgcttccca gtcggtccag atcttctcgc agacctccgg atcatgtccg ttggctttgc
     2161 aaccgtccat gaacttcgac ttcatcttgt cgagtacgtc cttctgcttc ttgcccatcg
     2221 ccttccgcag cgtatccgcg tcgcccttgg tgaatccggc cagtttctgc gacagcagca
     2281 tgacctgctc ctgatagacg gtgatcccgt aagactcctg aaggtattct tccatcgccg
     2341 gcaggtcgta ggcaaccgcc tcccggccgt gtttacgtgc gatgaagttg gggatgtatt
     2401 ccagcgggcc cggacggtag agggcgttca tggcgatgag gtcctcgaac ttgtcggggc
     2461 gcaactcctt gaggtatttc tgcatgccgg cggattcgaa ctggaacgtt ccgtttgttt
     2521 cgccgcgttg gtagagttca aacgtcttgg tatcgtcgag cgggatggcg tcgatatcga
     2581 tcttgacacc gtgattctgc tcaatgagac gcagcgcatc acgaatgatc gtcagggttt
     2641 tcagcccaag gaagtccatc ttgatgacgc cggcatcctc aatgactttg ccatcatact
     2701 gggtgatgag caattccgta tccttcgagg tggcgaccgg gatgatctcg gtgagatcct
     2761 tcggcgcgat gatgatacca gcggcatgga tgccggtatt ccggaccgac ccttccaatc
     2821 ggagggcttc ccgcaacacc gccgcttgca gatccttgcc tttatagatc tcacgcaggc
     2881 gcttgacctg ttccagatcg tcgctgctga ggttctcttt ttcccggagg cttttttctc
     2941 catcgagcgg ggcttcgagt acccgctgga gttcgatgcc tggccgctcc ggcaccagct
     3001 tggccagggc gttggattcg ggcaatggaa gatccaggac acgcgcaacg tccttgatgc
     3061 tcatcttggc ggccatggtg ccgtaggtga cgatctgggc cacctggttt cgtccgtact
     3121 tcgtcacgac atagtcgatc actttctgac ggcctacatc gtcgaagtcg gtatcgatat
     3181 cgggcatgct cttgcgatcc ggattcagga atcgctcgaa cagcaggttg tacttgatcg
     3241 gatcaatatt cgtaatgccg atgcaatacg ccacggcaga accggcggcc gatccgcggc
     3301 ctggtccgac gaaaacgccg aggtcgcggc cggccttgat gaaatccgca acgatcagga
     3361 agtagcccgc gaagcccatc gtcttgatcg taaacaactc gaagttgagt cgctcttcgg
     3421 tttccggggc gatatcgcca taacgctttt tcgcgccctc gaaggtgatg tgccgcaggt
     3481 attcgtcctg gtcggtaaat cccttcggca actgatagtg cggcagcagg atgtcctgct
     3541 tcagccggag cgtatcgacc ttggagacga tctcgttggt gttgtcgatc gcttccggca
     3601 catcggcgaa gagggccgac atttcgtcgg tcgtcttgaa atagaactgg tcgttgtaga
     3661 aagcgaagcg ttttcctttc gtaaaggcat cgtcgtcgct gaactccttc atggtaggag
     3721 tcgcctgttt ttctcctgtg ttgatgcaca gcaggatgtc gtgggcattg taatcatcgc
     3781 gatcggtata atgggaatcg
//
LOCUS       DAOTON010000176         3795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19277_length_3795_cov_4.237166, whole genome shotgun sequence.
ACCESSION   DAOTON010000176 DAOTON010000000
VERSION     DAOTON010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19277_length_3795_cov_4.237166"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="P5292_04635"
     CDS             complement(<1..576)
                     /locus_tag="P5292_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HRS38447.1"
                     /translation="MMRIAIVGTRGIPAAYGGFETLAERLALGLHALGHQVTVLGNSG
                     SAHDRWQGIKRIPTRFQKQDHPVLFYLESLRLSRQGFDRVLVCGVGGAPFYSLYPMKE
                     GVRITHVDGLEHLRDKFSSVKKWYVRKAQRLLCRSSAPVVSDSAAVTAYWREELEHKG
                     LLRTIMYGADIVTVADRSLLPESLVPGEYYLV"
     gene            complement(573..1637)
                     /locus_tag="P5292_04640"
     CDS             complement(573..1637)
                     /locus_tag="P5292_04640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS38448.1"
                     /translation="MDHYDLGLARGLAELGNEVNIHSNFTSDESHVRSFKSFGRGEVR
                     WWNLAALYFRYLQVLRKCKTNHVGTLIIHLFHFNQFDAWLVRKARRMGFYLIGIVHDV
                     EGFVRPTNNRRLLNIVQHGLDRMVVHNRYSRDELLRISGNKLGEKIRVIPHGQFAEIP
                     GEEAPVAGKESFRVGQEKEFTILFFGMIKPTKGLETLIGAMASLPPDVKLLVAGRTRP
                     GMKGSVIRDLQALEREGRARLFLHSIPPEAVSSFFQSADVVVLPYSRVYQSGVLLRAW
                     CEGIPVVLSDLPAFREVAAEGSDALFVPAGDRQALMKAIMRLKDDGSLRSSLSEGGRK
                     RLVRDHNWKDIAREFSNLLA"
     gene            complement(1688..2596)
                     /locus_tag="P5292_04645"
     CDS             complement(1688..2596)
                     /locus_tag="P5292_04645"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRS38449.1"
                     /translation="MSKKHYPDFLLVGAARAGTTAIDRYLRQHPEIFLPRVKEPCFFA
                     FAGMTTEYKRGKFSFAVTDSESYEKLFKDRKPNQLAGDLSTPYLFLYKQTLENIQRYH
                     PDPKRLRIVILLRQPVDRAYSQYRWRLRDGREELSFAEAVAAEPIRKREGFSFDYLYL
                     ERSYYAAALQAYRDQFPHVHIIFYEDLRKDPLLVMQGLCRFLGVDPEFPFQAIGEVNG
                     TWSPRYPWLSRLVTFEHPLKYRLLNRIPGHWRDSIRAGFTKINAVKHAEEQLDPEYRR
                     QLTERFTEDILAVQSITGRNLASWLQ"
     gene            complement(2593..>3795)
                     /locus_tag="P5292_04650"
     CDS             complement(2593..>3795)
                     /locus_tag="P5292_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38450.1"
                     /translation="STFRVFATLPLETLHQTLSSVAIISLTLYYIGSVYVDLRASRLT
                     WLDVLMWIFILVNFFAAYQGRVVFGQPYYFGIMAQRSVLLSISGVLLISMMNRGMIHI
                     RQVERTFVVLSLVLLAVFYFFFLFVNPVKFLDAEFVAYSPIRGYRYRFQFALVIMLLF
                     YSLFKISLEKRSRYYIVVLLILFYLIWYLQSRTTLAVLAITLLIFFVRNFSLREKFRN
                     TVLYGGFALVVVAIFFSLGYTSLFDKYQVLYKNVFNVFIGESPDEASSAVRFMEMNIA
                     LDYISKNPLLGNGFISNQWNGGWHDILGYFYPVDIGLLGNIFVFGVIGTTLLYLPYAY
                     SYTLAKQIRSNDVFFKTCEYMLLFFFLSMFFSAVNIRDSSSIMFLVCLLYYFRFYYFT
                     ANERAVVG"
BASE COUNT         1058 a         1051 c          909 g          777 t
ORIGIN      
        1 cacgaggtaa tactcgccag gcacgagcga ttccggcaac agcgaacgat ccgcaacagt
       61 cacgatatcc gctccataca tgatcgtacg cagcaagccc ttatgctcca actcctcacg
      121 ccaataagcg gtgacggccg ccgaatctga aacgaccggg gcggaactac gacacaacaa
      181 tctctgcgct ttccgaacat accatttctt cacgctggaa aatttgtcac gaagatgctc
      241 cagtccgtcg acgtgcgtga tgcgaacgcc ttccttcatt ggatagagcg aatagaatgg
      301 cgctcccccc actccacaga ccaatacgcg atcaaatccc tgcctggaaa gtcgaagact
      361 ttcgaggtag aacagtacgg ggtgatcctg cttctgaaag cgggtaggaa ttcttttaat
      421 tccctgccac cgatcatgcg ccgaaccgga atttcccagc acggttactt gatgaccaag
      481 cgcatgaagt ccgagcgcaa gacgctcggc aagtgtttca aaaccgccat aggctgcggg
      541 gatgccgcgg gtacccacga tcgcgatgcg catcatgcca gcaagttgga aaattcccgt
      601 gcaatatcct tccaattatg gtcgcgcacc aatcgcttgc gaccaccctc cgacaaggat
      661 gaacggagtg aaccgtcatc cttcaaacgc atgatggcct tcatgagggc ttgtcgatca
      721 cctgccggaa cgaagagcgc gtccgacccc tccgctgcca cctcccgaaa tgcaggcaga
      781 tcggaaagta ctactggtat tccttcacac caggcgcgta gcaagactcc gctctggtag
      841 acacgactgt atggcaagac aacaacatcg gcagactgga aaaacgaact gaccgcttcg
      901 ggaggaattg aatgaagaaa aagtcgagca cgcccttcac gctcgagtgc ttgcaaatcc
      961 ctgatcacag accctttcat accaggccga gtccgtccgg cgaccaacaa cttcacatcg
     1021 ggcggcaacg atgccatcgc accaattaag gtttccaaac ccttcgtcgg tttaatcatg
     1081 ccgaaaaaca ggatcgtgaa ctccttttcc tgaccaaccc tgaatgactc cttcccggca
     1141 acaggcgcct cctcacccgg aatttccgcg aactgaccgt ggggaatgac cctgatcttt
     1201 tcgcccaact tgttgcctga aatacgcaac aactcatcac gactatatcg gttatggaca
     1261 accatccggt ccaaaccatg ctgaacgatg ttcagcaatc gccggttgtt cgtgggccgg
     1321 acaaaccctt caacatcgtg gacgataccg atgagataaa aacccatccg ccgggctttt
     1381 ctcaccagcc aggcgtcgaa ctggttgaaa tgaaacaagt ggataatcag cgttcctacg
     1441 tgattagtct tacacttcct caaaacttga agatatctga aatacaaggc cgccaaattc
     1501 caccaacgca cttctccacg cccgaaactt ttaaaggacc gcacgtggct ttcatcgctg
     1561 gtgaaattgg aatgtatatt cacttcattt ccgagttccg ctaatcctcg cgccaaaccg
     1621 agatcatagt gatccatacc cgccttcatg cccaccggat ccaggatagc aagcctgcca
     1681 cgcgatttca ttgcagccat gacgcaagat ttcttccggt tatcgattgt accgccagga
     1741 tatcctcggt gaaccgctcc gtgagctggc gacgatactc cggatccaac tgctcctctg
     1801 cgtgtttaac agcattgatc ttggtaaagc cggcacggat ggaatcacgc caatgtccag
     1861 gaatcctatt aagtagccgg tatttcaacg ggtgctcgaa agtgaccaac ctgctcaacc
     1921 agggataccg tggcgaccag gtcccgttca cttccccgat ggcttgaaac ggaaactccg
     1981 gatcgactcc caggaatcta caaagccctt gcatcaccag cagcggatcc ttccgcaggt
     2041 cttcataaaa aataatgtgt acatgaggga attgatcccg gtaagcttgc aaagccgcag
     2101 cataatagga acgttccagg tagagatagt caaaactgaa tccttctctt ttccggatcg
     2161 gctccgccgc taccgcttca gcgaaactca actcctcccg gccatcccgc aagcgccagc
     2221 gatactgtga ataggcccga tcgaccggtt gacgcaacag gataacgatt cgcaatcgtt
     2281 tcggatcagg atgataccgc tgaatgttct ccagggtctg cttgtaaagg aacaggtagg
     2341 gtgtggaaag atctcccgcc aattgattgg gctttcggtc cttgaagagt ttttcataag
     2401 attcggagtc cgtcacggca aacgagaact ttccccgctt gtattcagtt gtcattcctg
     2461 caaaagcgaa aaaacagggc tccttgacac ggggtaagaa aatttccgga tgttgtcgca
     2521 aataacgatc aatggcggta gtaccggctc gcgcagctcc cacgagaaga aagtccggat
     2581 aatgcttctt gctcatccga ctacggctcg ctcgttcgca gtgaaataat agaaccggaa
     2641 ataatacagc aggcaaacaa ggaacatgat gctgctcgag tcccggatat tcacggcaga
     2701 gaagaacatc gacaggaaaa agaacaacaa catgtattca caggttttga agaatacgtc
     2761 gttggaccgg atctgctttg ccagggtata cgagtaggcg tagggcaggt aaaggagcgt
     2821 agtgccgatc acgccgaaca cgaaaatatt gcccagcaag ccgatatcga ccggatagaa
     2881 ataacccagg atgtcgtgcc agcctccatt ccattgattg ctgatgaatc cgttacccaa
     2941 cagcgggttc ttactgatat aatcgagggc aatgttcatc tccataaaac ggaccgcgga
     3001 gctggcttcg tccggcgact ctccgatgaa tacgttgaag acattcttat acagtacttg
     3061 gtatttgtca aacagcgagg tatagcccaa gctgaaaaag atcgcgacca caaccagcgc
     3121 aaagccccca tacaatacgg tgttccggaa tttctcgcgg agagaaaaat tccggacgaa
     3181 aaagatcagg agcgtgatgg ccagtaccgc cagcgtcgtc ctcgactgca ggtaccaaat
     3241 caggtagaac aggatcaaca ggacaacgat gtaataccgg ctgcgttttt ccagggagat
     3301 cttgaacaag gagtaaaaca ggagcatgat caccagcgca aactgaaacc ggtaacgata
     3361 accccgtatc gggctataag ccacaaattc cgcatcgagg aatttcaccg ggttgacgaa
     3421 caggaagaaa aaatagaaca ccgccaacag gaccagggac aatacgacaa atgtccgttc
     3481 cacctgccgg atatggatca tcccccggtt catcattgaa atcagcaaca cgcccgagat
     3541 cgacaacaag acggatcgct gcgccatgat gccgaagtaa tacggctggc cgaagacaac
     3601 cctaccctga taggccgcga agaaattgac caggatgaag atccacatca atacatccag
     3661 ccaggtcagg cgggaggcgc gcagatcgac atagacgctg ccaatataat aaagtgtcag
     3721 gctgatgatc gcgacggaag aaagcgtctg atgaagggtt tcgagcggta aagttgcgaa
     3781 gacccgaaat gtcga
//
LOCUS       DAOTON010000177         3787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19321_length_3787_cov_2.989818, whole genome shotgun sequence.
ACCESSION   DAOTON010000177 DAOTON010000000
VERSION     DAOTON010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19321_length_3787_cov_2.989818"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="P5292_04655"
     CDS             complement(<1..479)
                     /locus_tag="P5292_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRS38451.1"
                     /translation="MSTEITKEATIGVLGAGTMGNGIAQVAAGAGHRVILLDANAAVL
                     DKARAIHHQVLDKLVAKGKLTPEAAKANLSRIHYAANLDAFAPCMLVIEAIVEDLDVK
                     RKVFAEIERWVAADAVIATNTSSLSVTSIAAACKQAERVLGVHFFNPAPLMPLVEIVP
                     "
     gene            690..944
                     /gene="rpsT"
                     /locus_tag="P5292_04660"
     CDS             690..944
                     /gene="rpsT"
                     /locus_tag="P5292_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRS38452.1"
                     /translation="MANHKSALKRIRNSEAKRNRNRYQAKTMRNALKNFRLLKNKKEA
                     AAALPGLASMLDRLAKKNVIHKNKASNLKSELAIHMNHLS"
     gene            1065..1760
                     /gene="radC"
                     /locus_tag="P5292_04665"
     CDS             1065..1760
                     /gene="radC"
                     /locus_tag="P5292_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRS38453.1"
                     /translation="MEHVTGSIKEWAEADRPREKLMQKGRQSLSDAELLAILIGSGTR
                     HESALEIARRILTKVDNNLHALGLMPLNELRSFRGIGNARAIIIQAALELGLRRTRDE
                     TRPPQDKIRGAADVAAQFRPLLCDLMHEEFWVLLLNRANTIIGKEQISKGGLTGTVAD
                     PRLIFRSAIDRRAAAIVVCHNHPSGNLSPSEQDIRLTKNLVEAGKVLEIPVLDHIIIT
                     QHGFYSFADEGRI"
     gene            1790..3094
                     /locus_tag="P5292_04670"
     CDS             1790..3094
                     /locus_tag="P5292_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRS38454.1"
                     /translation="MLLVFVRSITPRLRYTFDFIFRDILGMEYSFTQDADQFSFHTGP
                     RMSYTDRPVGDEPFFFATSLLFEKGVHQQEISVFDWESGKVFFATHPKFLLPFDPFAA
                     TFYLVSRYEEYLPHKRDQHDRFDARESLAYQKGFLHLPVVDQWAYRIRDLLLHHFPGL
                     TFQRRSYQYISTVDIDNAWAYREKGFVRTVGALGKALFRFDFSSMVDRCAVLLGSRKD
                     PYDTYELLNILQQRYRYPSIYFVLLGDYGEYDRNVSPYRRAFQSLIKSLADYNRCGIH
                     PSYRSGGDAERVRLEKGRLRKMIRRDVTDSRQHFLRIRFPDTYRNLIDCDITDDYSMG
                     YANETGFRAGTCTPFYFYDLEQEHPTHLRIHPFAVMDATLRFYQRVRPEEAMGTVGPL
                     IKAVKAVDGNFVSLWHNESLSEREPWVGWRTVYEELVKSASA"
     gene            3096..>3787
                     /locus_tag="P5292_04675"
     CDS             3096..>3787
                     /locus_tag="P5292_04675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS38455.1"
                     /translation="MIRFLRHEEIDRHRWDEVITHSPEGTIYAESWFLDAVCPGWHAL
                     VEDDYKAVFPLTWRKKYGLHYLFQPPFCQQLGLFSKQETPLIDAAPFLEAIPKHYLLV
                     ELQLNSGNRCTHPAFTVTERPNLELRLQRDYAALAKGYSDNTSRNLRKAQQSGLVVET
                     ADSIEPVIRLFRDNRGSTLERFDEQQYERLRSLAGSALEKGRGIIRLVRDPSGQLHAG
                     AFFLQSKDRLIFL"
BASE COUNT          857 a         1055 c         1030 g          845 t
ORIGIN      
        1 ggaacaatct cgaccaacgg catgagcggt gcgggattga aaaagtggac accgagcaca
       61 cgttccgctt gcttgcaggc agcagcaatg ctggtgacgg aaagcgaaga agtgttggtg
      121 gcgatcaccg catccgccgc tacccagcgt tcgatctccg cgaatacttt ccgtttgaca
      181 tccaaatcct cgacaatggc ttcgattacc agcatacagg gcgcgaatgc gtccaggttg
      241 gcggcatagt ggatgcggga aagattggct ttcgccgctt cgggcgtcag ttttcccttg
      301 gctaccaatt tatccagtac ctggtgatgg atcgcccgag ccttatccag cacagcggca
      361 tttgcgtcga gtagaatcac cctgtggccg gcgccggcgg caacctgtgc gatgccattg
      421 cccatggtac cggctcccag cacgccaatg gtggcttctt tcgttatttc agtgctcatt
      481 cgaagtacaa aagtagcaat aaatcgaggg tgccattttt acacggaatc gcccgattag
      541 tcatatatta ttgattatta agtatttaaa tgaatgtcac caatttgaag aaaagcatcg
      601 gctggtattt ggttcaatag ttccggaatc gtatatttgc actccttttc cgcgatattg
      661 gcggaattta ccaacagcat tcattaccca tggcgaatca taaatcagca ttgaagcgca
      721 tccgcaacag cgaagcgaag cgcaaccgca accgttacca ggctaaaacg atgcgtaacg
      781 ctttgaagaa tttccgtttg ttgaagaaca agaaagaagc agcagccgca cttccgggac
      841 ttgcctccat gctggaccgt cttgccaaga agaacgtcat tcacaagaac aaggcgtcca
      901 acctgaagag cgagttggcc atccacatga accacctgag ctgatcagtt caacgatatt
      961 cctgaaaagg ctgtctttcc cgacagcctt tttttttggc cctgctgaat tattccgcat
     1021 ctattggatg cagcattgca gacgactcgc caacttgccc gaccatggag catgtaaccg
     1081 ggtcgatcaa agaatgggcg gaagccgacc gtccgcgaga aaagctgatg caaaaaggcc
     1141 ggcagtcgct cagcgatgcg gaactgttgg ccattctgat cggctcgggt acccgacacg
     1201 aatcggcgct cgagatcgcg cgccgcatcc tcacgaaagt cgacaacaac ctccacgcgc
     1261 tgggtctgat gccgctgaac gagctgcgtt cattccgggg tatcgggaac gcccgggcga
     1321 tcatcatcca ggctgcgttg gaacttggac tccgacgcac gcgcgacgaa acccgtccgc
     1381 cgcaggataa gatccgcggc gccgccgatg tcgcggccca attccggccg cttctctgcg
     1441 acctgatgca cgaagaattc tgggtgctgc tgctgaacag agccaatacc atcatcggta
     1501 aggagcaaat cagcaagggc gggctgacgg gtaccgtagc cgatccgcgg ctgatcttcc
     1561 gctccgccat cgatcgtcgg gctgcggcca tcgtggtctg ccacaaccat ccgagcggga
     1621 acctgagtcc gagcgagcag gacatccggc tgacgaaaaa cctggtggaa gccgggaagg
     1681 ttttggagat accggtcctg gatcatatca tcattacaca gcatggtttt tatagctttg
     1741 cggatgaggg ccggatctga gcaggaagac tccccggccc acccgtttca tgttgctggt
     1801 attcgtccgc tcgatcactc cccgactccg gtacaccttc gatttcattt tccgggacat
     1861 tctcggaatg gagtattcct tcacacagga cgcggatcaa ttctcctttc atacggggcc
     1921 gcgcatgagt tatacggacc ggccggtggg cgacgaaccc ttcttcttcg cgacatcgtt
     1981 gctgttcgaa aagggtgtcc atcagcagga gatctcggtc ttcgactggg agagcggtaa
     2041 agttttcttc gcgacacacc cgaaattcct cttgcctttc gatcctttcg cggcgacttt
     2101 ttatctggtc agccggtacg aagaatacct gccacacaag cgcgaccagc acgaccggtt
     2161 cgacgcgcga gaaagtctgg cctatcagaa aggtttcctg cacctgccgg tagtcgatca
     2221 atgggcctac cgtatccgcg acctgttgtt gcatcacttc ccgggtttga cgtttcaacg
     2281 cagaagctat cagtacattt ctacggtgga catcgataac gcctgggcgt atcgggagaa
     2341 aggattcgtg cgaacggtcg gagcgctcgg caaagccttg ttccgattcg acttctcttc
     2401 catggttgat cgttgcgcgg tcttgttggg cagccggaaa gatccgtacg atacgtacga
     2461 gttgctcaac atcctccagc agcgctaccg ctacccgtcg atctatttcg tcctgttggg
     2521 tgattatggc gaatacgatc gaaacgtatc gccgtatcgt cgtgctttcc agtctttgat
     2581 caagtcgtta gccgactaca accgttgcgg tatccacccc tcgtatcgtt cgggtgggga
     2641 tgcggaacgt gttcggctgg agaaaggacg gctgcggaag atgattcgac gcgacgtaac
     2701 cgacagccgg caacattttc tccggatacg ctttccggat acctaccgca atctgatcga
     2761 ctgcgatatc accgacgatt acagtatggg gtatgccaat gaaaccggtt tccgtgccgg
     2821 gacgtgcacg cctttctact tttacgacct ggaacaggaa cacccgacgc atctgcgcat
     2881 ccatcccttc gcggtgatgg atgcgaccct gcgcttctac caacgcgttc gtccggagga
     2941 agcaatgggg acggtcgggc ccttgatcaa agccgtgaag gcggtcgatg gcaacttcgt
     3001 ttccctctgg cacaacgaat cactcagcga acgcgagccc tgggtcggtt ggcgaactgt
     3061 ttacgaagaa ctggtaaaat cggcgagcgc ctgatatgat ccgcttcctg cgtcacgagg
     3121 aaatcgaccg ccatcgttgg gatgaggtca ttacccattc tccggaaggg accatctatg
     3181 cagaaagctg gtttctggac gcggtttgtc cgggctggca cgccttggtt gaagatgact
     3241 acaaagccgt cttcccactg acctggcgaa agaaatacgg actgcactat ctcttccagc
     3301 caccgttctg tcagcaattg ggactcttca gcaaacaaga aacgccgctg attgacgcgg
     3361 caccctttct cgaggccatt ccgaaacact accttttggt agagttgcag ttgaacagcg
     3421 ggaatcgttg cacgcatccc gcgtttaccg tcacggagcg acccaacctg gagttgaggt
     3481 tgcagcgcga ctacgcggca ctggcgaaag gctattcgga caataccagc cgcaacttgc
     3541 gtaaggcgca gcagtcgggg ctggtcgttg aaacggcgga ttcgatcgag ccggtcatcc
     3601 gattgttccg ggacaaccgg ggaagcacac tggagcgttt tgacgaacag cagtacgagc
     3661 gactgcgcag cttagccggc agtgcactgg aaaaaggcag gggaatcatc cgccttgtcc
     3721 gggatccaag tggacagttg catgccggcg ccttcttctt acaaagcaaa gaccggctga
     3781 tctttct
//
LOCUS       DAOTON010000178         3758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19473_length_3758_cov_3.248177, whole genome shotgun sequence.
ACCESSION   DAOTON010000178 DAOTON010000000
VERSION     DAOTON010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19473_length_3758_cov_3.248177"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="P5292_04680"
     CDS             <1..519
                     /locus_tag="P5292_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38456.1"
                     /translation="AEKRWVVGHLFVAKRAYRISLEARDRATKLEQDARLDHDKDGGE
                     VDAFRHAYWMARLTQSIGRKKALKLGEAHELGNYQDYLSGKVEEVHLPDSMSSVMDRW
                     NNRLGAGIGEGCAHFSKEQLEQRLIDEIRHGRARKLRKNGQGEFLDCAGQRVDRSLRT
                     WRKETCLIPTGR"
     gene            complement(532..1650)
                     /locus_tag="P5292_04685"
     CDS             complement(532..1650)
                     /locus_tag="P5292_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38457.1"
                     /translation="MRILLLILLLLHGLIHLIGFVHEFQLANVRQLRGKLSVPVGSIG
                     LKVLGVLWLATTVLFLLGAVQVLRSQENWSILVLIAIALSQLLILLYWKDAKAGMAAN
                     ILLLLLALPALKEVRFKSENESLKQAMLDQAEIFQPDSNRMGELPAPVQAWLLSHAGG
                     HLSGKRYVSLKQTGSMRSKTDGKWVPFQAEQTVTCNPAAFQWSVRMDMAPGLFVLGRD
                     RFENGKGRMLIRMLGLFSIADAQGPETDQGTLVRYLAEMIWYPWFALSPDILWETIDE
                     HRAMATLRIRGTEGSGVFTFNDQHRPVAFNAQRYYDRKGTSTLEHWHVEMDPTSYREF
                     DGVSVPTKSVVRWDLEGGKFEWLQLEVTSMTIRENRQP"
     gene            complement(1734..2228)
                     /locus_tag="P5292_04690"
     CDS             complement(1734..2228)
                     /locus_tag="P5292_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38458.1"
                     /translation="MMKTPASRLAFLLLILLHLTLGFSAIGGGLLLMLEPDGGLLHMQ
                     QGWLHGSVFPNFFLPGLMLTTVIGIIPLRVAFGLNSGLKRCSPGYINVYRSRHWAWTW
                     SLYYGIILCAWIAIQQLLTQYFILQPVILLSGILIIVLTLTPSLMHRYHMNPREQLEA
                     ETRE"
     gene            complement(2308..3066)
                     /locus_tag="P5292_04695"
     CDS             complement(2308..3066)
                     /locus_tag="P5292_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38459.1"
                     /translation="MRYFTALLLFFSTIAFGREPRTLKTDTGQVVLHFHRNGHTSTVE
                     WTDLAGRYGVSYALDAKGKEILRLQTRRFAGHATVHFSYHANGSVALAEYSNAPDAGI
                     QWYRATYRFDESGNQTGFEEQGHDNYGPVPRPGIRPSERMPAPREACQEPVVNTYFIV
                     NPLDYPVLVHLLPKHPSPASKDTACIVYPADTLKGGAWITGDATGDVGAVLDWRLTAL
                     KNGSLNIQPIIVKTEIRVQGSRQRDHFLVLGVGR"
     gene            3308..3673
                     /locus_tag="P5292_04700"
     CDS             3308..3673
                     /locus_tag="P5292_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRS38460.1"
                     /translation="MKNALNWFEIPVKNFERAKTFYSKLLGTEIGEMPFPDGRYGIIA
                     WDQEGVGGGLVQCEGYEPSDKGTIVYLNGGEDLSGPLSRVETAGGKILLPKTSIGQNG
                     FMAHFMDTEGNRVALHSMK"
BASE COUNT          918 a          975 c         1030 g          835 t
ORIGIN      
        1 gcggaaaagc ggtgggtcgt cgggcacctg tttgtcgcga aacgcgctta ccgtatttcg
       61 ctggaggcgc gtgaccgtgc aacgaaattg gaacaggacg cgcgactgga ccatgataag
      121 gatggagggg aagtggacgc gtttcggcat gcttactgga tggccagact cacgcagtcg
      181 atcgggagga agaaagcgtt gaaactcggc gaagcccatg agttgggtaa ttatcaggac
      241 tatctaagcg gaaaagtgga agaggtccat ctgcccgatt ccatgtcatc ggtgatggac
      301 cgctggaaca accgattggg cgcgggcatt ggcgaaggtt gcgcgcattt ttcaaaagaa
      361 cagttggagc agcgcctcat cgacgagatc cgccacggcc gtgcccgaaa gctgcgcaag
      421 aacggacagg gtgaatttct cgactgcgcc ggacaacgtg tagatcgttc gctgcgtacc
      481 tggcgaaagg agacctgcct gataccaacc ggccggtgaa tgaatacacg atcagggttg
      541 acgattttcc cgaatggtca tgctcgtgac ttccaattgc aaccactcga attttccacc
      601 ttccagatcc caacgcacga cactttttgt cggaactgaa acaccatcga actcccggta
      661 ggaagtcggg tccatctcca catgccaatg ttcgagcgtc gaggttcctt ttcggtcgta
      721 ataacgttgt gcattgaacg ctacgggacg atgctgatcg ttaaaagtaa aaactccgct
      781 gccttccgtt ccccggatgc ggagagttgc cattgcgcgg tgttcatcga ttgtttccca
      841 aaggatgtca ggtgataaag cgaaccaggg gtaccagatc atttccgcga ggtaacgaac
      901 cagcgtcccc tggtcggttt ccggtccctg cgcgtcagcg atactgaaca gtcctaacat
      961 cctgatcaac attcggccct tgccgttttc aaaccgatca cgacccagca cgaacaaacc
     1021 gggcgccatg tccatacgga cactccattg gaaagccgca ggattacagg tcaccgtctg
     1081 ttccgcctgg aacggcaccc actttccatc cgtcttgctc cgcatactgc cggtctgttt
     1141 cagcgaaaca tatcgctttc cggagaggtg gcctccggca tgtgaaagta gccaggcttg
     1201 aaccggcgct ggtagttcac ccatccgatt ggaatccggc tggaaaattt ctgcttgatc
     1261 gagcattgcc tgcttcaacg attcgttctc cgacttgaag cgcacttctt taagtgcagg
     1321 cagtgccagg agcaagagca ggatattcgc cgccatgccc gctttggcat ctttccagta
     1381 gagcaggatg agtagctgtg aaagcgcaat cgcgatcaat accaggatag accagttttc
     1441 ctgtgaacgc aacacttgta ctgcgccgag caggaataaa accgtcgttg caagccacag
     1501 tacacccaac accttcaagc cgatcgaacc tacgggaacg gatagcttgc cacgcagttg
     1561 tcggacattt gccaactgga actcatgcac gaaaccgatc aggtggatca ggccatggag
     1621 cagaaggagg atgagtagta aaatgcgcat gatttgggtt gattgttccg ggttccgagt
     1681 ttcgggtcat agtagtcaac ccgaaacccg gaactcggaa ccctgtacca ccctcattct
     1741 cgggtctccg cttccagttg ttccctgggg ttcatgtggt agcggtgcat cagggagggt
     1801 gtcagtgtga ggacgatgat caggatcccg gagagcagga tgaccggctg caggatgaag
     1861 tattgggtca gcaattgctg gatagcgatc caagcgcaca ggatgattcc gtagtagagt
     1921 gaccaggtcc aggcccagtg tctggagcgg tacacattga tataaccagg actgcaacgc
     1981 tttaaaccgc tattcagccc gaatgcgaca cgtaagggaa tgatcccgat cactgtggtg
     2041 agcatcagtc ccggcaggaa gaaattcgga aacacggatc catgcaacca cccttgttgc
     2101 atgtgcaata gcccgccgtc gggttccagc atcagcaaaa gtccgccacc gattgccgaa
     2161 aagcccaggg tcagatgcaa caggattaaa agtaaaaatg ccaggcgcga agcaggtgtt
     2221 ttcatcatca atcgaacgac agttttaaaa atatcaatag ctgaatgcgt acgctttgat
     2281 gaagatcaga aaaaatactg acttccgtca ccggccgacc cccaggacca gaaaatggtc
     2341 gcgttgcctg gagccctgaa cccgaatttc tgttttgaca atgatcggtt ggatgttcaa
     2401 actcccgttt ttcaaggcgg tcagccgcca gtctaaaaca gcaccaacat ccccggtggc
     2461 atcccccgtg atccaggctc cgcctttgag tgtatcggcc ggatagacaa tgcaggcagt
     2521 gtccttcgat gcaggtgaag gatgcttcgg tagcaggtgg accagaacgg gatagtccaa
     2581 cggattcacg ataaagtacg tgttgaccac cggctcctga caggcttcgc gcggtgccgg
     2641 catccgctca gatggcctta tcccgggcct tggcaccgga ccgtaattat catgtccttg
     2701 ttcttcaaag cctgtttgat ttcctgattc atcgaagcga tacgtcgccc ggtaccactg
     2761 gataccggca tccggcgcat tcgaatattc ggccagcgct accgatccgt ttgcatgata
     2821 ggaaaagtgt actgttgcat gtcccgcgaa gcgccgtgtc tgtaagcgca ggatttcttt
     2881 ccctttcgca tcgagcgcgt aactgacgcc atagcgacct gcaaggtccg tccattccac
     2941 cgtcgaagta tgcccgttcc ggtgaaaatg caggaccacc tgtccggtgt cggttttcag
     3001 ggttctgggt tcccgcccga aggcgatggt agagaagaag agcagcaggg ctgtgaagta
     3061 acgcatgtgg atcgctaacc aacgattgtg ccggagctta ggtacagcag aagggcggaa
     3121 accagggtgc cctgcttgct ctccccccag tccttatgcg gttacggtaa cttgaaactt
     3181 gtaactgtgg tttgtttctt tcgcaatgca tgaatttgta acttccacgg cacaggtatg
     3241 atggccggac aatcaaccaa caatgcatga gccttcggct attcaacaat caatttcaac
     3301 tgacacgatg aaaaacgcac tcaactggtt tgaaataccg gtcaagaatt tcgaaagggc
     3361 gaagacattt tattcgaagt tgctgggcac cgagattggc gagatgccgt ttcccgatgg
     3421 tcgttacggg atcatcgcct gggatcagga aggggtaggc ggcggactcg ttcaatgcga
     3481 aggatatgaa ccatcggaca aagggacaat tgtctacctg aacggaggcg aggacctcag
     3541 cggtccgctt tcgcgggtgg aaacagcagg aggaaagatc ctgctgccca aaacgtccat
     3601 cggtcagaac ggtttcatgg cccacttcat ggatactgaa ggaaaccggg tggcgctgca
     3661 ttcgatgaag taatgcattg catggaaatg gatgggatgt atttccatgc tgacctgttt
     3721 gctttatcta acagtatata caaggatgcg cttctccg
//
LOCUS       DAOTON010000179         3755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19493_length_3755_cov_3.237838, whole genome shotgun sequence.
ACCESSION   DAOTON010000179 DAOTON010000000
VERSION     DAOTON010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19493_length_3755_cov_3.237838"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1984
                     /locus_tag="P5292_04705"
     CDS             <1..1984
                     /locus_tag="P5292_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HRS38461.1"
                     /translation="GPSNTNCLFNGEQGGEGWSDYFALLFTIEPGDQGSDSRGIGTYA
                     FDEPTTGGGIRRYPYSTDMGINPQTYGDLAGSGAVHDIGEIWCGTLWDMTWNLIDVEG
                     FDPDWINGTGGNNIALRLVLEGMKLQPCGPGFLDGRDAILLADDNLYSGAHRCLIWNA
                     FARRGMGASAQQGSANNTGDEVENFDLPTICLTPVAPPVANFISNVTNTCYGIVFFTD
                     QSTDIPQSWFWDFGDGSTSTAQNPSHTYLSSGSYTVTLIVTNTLGADTLVRTNLITVA
                     YPTSPSISGDNAICSGETTTLTASVSPSYDVTWETSGGTVVGTGTTFTTPALTATTTY
                     RAIASTQSSVVNVGPPDASFGGGGYHATTFEGREIFTTFAPMRLRSVWVDASGTADRT
                     INLYNQSGTILQTLTVNIPNGQSRVQVNFDIPTPGNYEIGVVAGSNLYRNNSGASYPY
                     TIGGLVSITSSNSTTNPATYYYYLYDWEVQELPCESTPATYTINVSAGPSSNYTYSAT
                     GLTVQFTDNSGGSPVSWSWDFGDGNTSIQQNPSHTYAANGTYSAVLTVTDANGCTNTT
                     QQSITVTSVGIADPGTANWQILQQDRTLIVRFPLSEQVQARITVLDITGRELLRSETR
                     QHEWRVALDRFAAEVLLVRIEQGGRTEYRKLFTDRY"
     gene            2120..2686
                     /locus_tag="P5292_04710"
     CDS             2120..2686
                     /locus_tag="P5292_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRS38462.1"
                     /translation="MEFNLRDTLTVSTILFAVIDIIGSIPALLQVKTRVGYIKAEQAT
                     FVSLIIMVVFLFLGESILAFLGVDVRSFAIAGSLVIFFLALEMILGIRLYRDEIPETA
                     SIVPVAFPLIAGTGTMTTLLSLRAVYDMPTIVIGIFLNLIVVYVVLRNLYRLEKLLGA
                     AGISVLRKVFGIILLAIAVKLFRTNVGL"
     gene            2814..>3755
                     /locus_tag="P5292_04715"
     CDS             2814..>3755
                     /locus_tag="P5292_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS38463.1"
                     /translation="MKKRLPNVWMLLAAMLFAGISANAQVTYNICSTPAVVTDTAATL
                     YDTGGPSGDYQVNENCTLLVQPSCATSITLTFTSFETESGFDYFYVYDGTTIAAPQLL
                     AANGQGTIPGPVTCTSGAMLIIWRSDVSIVYSGFECSWTSVIAPSAAPTAAFTANDFT
                     PPLLTNVLFTDQSVGSPTTWLWDFGDGDTARSQNPVHAYQNPGTYTVTLIAFTCNESD
                     TITQQITVDAAPQIDVTPLGLSANAQCGDSVSFNLDISNIAGGELFWTTDNSNVSTGI
                     PVRVVAMTYGTDQFSEYPNTIDAIDQIGREVFGPVLHV"
BASE COUNT          782 a         1129 c          982 g          862 t
ORIGIN      
        1 tggcccatcc aacaccaatt gcctctttaa cggagagcag ggcggcgaag gatggagtga
       61 ttatttcgcc ttgttgttca cgatcgaacc gggcgaccag ggatccgatt cgcgtggcat
      121 cggaacgtat gcgttcgacg aaccgaccac cggcggaggg attcgccggt atccttactc
      181 caccgacatg ggcatcaacc cgcagaccta cggcgatctg gccggcagcg gcgcggtgca
      241 cgatatcggt gagatctggt gcggaacatt gtgggatatg acctggaacc tgatcgatgt
      301 ggaaggattc gatcccgatt ggatcaacgg taccggcggc aacaacatcg cgctgcgtct
      361 ggtgctggaa ggcatgaagc ttcaaccttg tggtcccgga ttcctggacg gtcgtgatgc
      421 catcctgtta gcggatgaca acctctacag cggcgcacac cgttgcctga tctggaacgc
      481 tttcgcccgc cgtggcatgg gtgccagtgc gcaacagggc agtgcgaaca ataccggtga
      541 cgaagtagag aacttcgacc tgccgaccat ttgccttacg ccggtggcgc ctcctgtcgc
      601 caacttcatc agcaatgtca ccaacacctg ctacggcatc gtgttcttca ctgaccagtc
      661 gacggacatt ccgcaaagct ggttttggga tttcggcgat ggttccacct caaccgcgca
      721 gaatccttcg catacctatc tcagcagtgg cagttacacg gttaccctga tcgtgaccaa
      781 cacgcttggc gccgataccc tggtgcgcac caacctcatt acggtggctt atccgacttc
      841 cccgagcatc tccggcgaca acgcgatctg ttcgggcgaa accacgacgc tcaccgcttc
      901 ggtttctccg agctacgacg tcacctggga aacttccggc ggtactgttg tcggcaccgg
      961 caccacgttc acgactcctg ccctgacagc taccacaact tatcgcgcca tcgcttccac
     1021 tcaatcgtcg gtcgtcaatg tcggcccgcc ggatgcgagc ttcggcggtg gcggttatca
     1081 tgccaccacc ttcgaaggtc gggagatctt caccaccttc gcaccgatgc gtctgcgatc
     1141 ggtatgggtg gatgcttcgg gaaccgccga ccgtaccatc aacttgtaca accagagcgg
     1201 tacgattctg cagacgctga ccgtcaacat tccgaacgga caaagccgcg tgcaggtcaa
     1261 tttcgatatc ccgacgcccg gcaattatga gatcggtgta gtagcgggat cgaacctcta
     1321 ccggaacaat tcaggcgcca gctatccgta caccattggc ggattggtca gcatcacctc
     1381 gtcgaactcg accaccaatc cggctactta ctattattat ctctatgatt gggaggtgca
     1441 ggaacttccg tgcgaaagca cacctgcgac ctatacgatc aacgtgagcg caggtccgtc
     1501 cagcaattat acctatagcg ctaccggact taccgttcag ttcaccgaca attccggcgg
     1561 cagccccgta tcgtggagct gggatttcgg cgatggtaac acgtcgatcc agcagaaccc
     1621 ttcgcacacc tacgcagcga atggtacgta ctctgcggtc ctgaccgtga cggatgcgaa
     1681 cggttgcacc aatacgacgc agcaatccat caccgttact tcggtcggca ttgctgatcc
     1741 cggaacggcg aactggcaga tcctgcagca agaccgtacc ctgatcgttc ggttcccgct
     1801 cagcgagcag gttcaggcgc gcattaccgt gctcgatatc accggacgtg aattgctccg
     1861 gtcggaaacg cgtcagcacg aatggcgtgt agcgctcgat cgttttgcag ccgaagtgtt
     1921 gctggtacgc atcgagcagg gcggacgtac cgaataccgt aaactgttca cagatcgtta
     1981 ctgatccgga ttcgcctact caacagaaaa ggccggaggg aatattcctt ccggcctttc
     2041 ccgttgatta cttccttcaa tgcgcgtccg gcgatcaggc cgctgtccct atcttggcgg
     2101 aaacgcaacc accctcccca tggaattcaa cctgcgcgat acgctgaccg tttcgaccat
     2161 tcttttcgcg gtgatcgaca tcatcggatc cataccggcc ttgttgcagg tcaagacacg
     2221 agtaggctac atcaaggcgg aacaggcgac cttcgtttcg ctgatcatca tggtggtctt
     2281 tctgttcctg ggcgaatcca tcctcgcctt tctgggtgtc gatgtccggt cgttcgccat
     2341 cgcgggttcg ctggttattt ttttcctggc actcgaaatg atcctcggca tccgcttgta
     2401 tcgtgacgaa attccggaaa ccgcctcgat cgttccggtc gctttcccct taatcgccgg
     2461 tacgggaacc atgacgaccc tgctttcctt acgggccgtt tatgacatgc cgaccatcgt
     2521 catcggtatc tttcttaacc tgatcgtggt ctatgtcgtg cttcggaact tgtaccgttt
     2581 ggagaaattg ttgggtgctg ccgggatcag cgtactgcgt aaagtgttcg gaatcatatt
     2641 actggctatt gcggtcaagc ttttcaggac caatgtgggc ctttgagccc cggttttaac
     2701 acttgaaacc ccgggaaacc cttatgggac ggggctttcc ggcctttccg ggatatttgt
     2761 ttttggagaa attatcctac atttggatac atttccaaaa agtccagacc gctatgaaaa
     2821 aacgcttacc caacgtttgg atgttactgg ctgccatgct tttcgctgga atttcagcga
     2881 acgcccaggt aacctataac atctgttcca cccccgcggt ggtaaccgac acggccgcaa
     2941 ccctctacga caccggaggt ccgagcggcg attatcaggt caacgaaaac tgtacacttc
     3001 tggtacagcc ctcgtgcgcg acctctatca cccttacgtt cacttcattt gaaacggaat
     3061 cggggtttga ttacttctac gtgtacgatg gtacgaccat cgccgctcct cagcttttag
     3121 ctgcgaacgg tcagggcacc attccgggcc ctgtgacctg tacatcgggc gcgatgttga
     3181 tcatctggcg ttcggatgta tcgatcgttt acagcggttt cgagtgtagc tggacttcgg
     3241 tgatcgctcc gagtgctgca ccgactgcag cgttcacggc aaacgatttc acgcctccgc
     3301 tgctgaccaa cgtattgttc accgaccaga gtgtcggttc accgaccacc tggttgtggg
     3361 atttcggtga tggcgatacc gcccgcagcc agaacccggt acacgcatat cagaacccgg
     3421 gaacctacac ggtcaccctc atcgcattca cctgcaacga gtccgatacg atcacccagc
     3481 agatcaccgt tgacgctgct ccccagatcg atgtcactcc gctcggcctc agcgccaatg
     3541 cgcaatgcgg tgattccgtt tcgttcaacc tcgacatcag caacatcgcg ggtggcgaac
     3601 tcttctggac aaccgacaac tccaacgtca gcaccggcat tccggtacgc gtggtagcga
     3661 tgacctacgg tacggaccag ttctccgagt acccgaatac catcgacgcc atcgaccaga
     3721 tcgggcgcga ggtgttcgga ccggtgctgc atgtg
//
LOCUS       DAOTON010000180         3749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19518_length_3749_cov_3.434488, whole genome shotgun sequence.
ACCESSION   DAOTON010000180 DAOTON010000000
VERSION     DAOTON010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19518_length_3749_cov_3.434488"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="P5292_04720"
     CDS             complement(<1..257)
                     /locus_tag="P5292_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRS38464.1"
                     /translation="MTDGEKIIPINIEEEMKTAYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLYGMLDLGVLSNRPYKKSARIVGEVLGKYHPHGDGSVY"
     gene            605..3181
                     /locus_tag="P5292_04725"
     CDS             605..3181
                     /locus_tag="P5292_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRS38465.1"
                     /translation="MEAKFSPRVKDVITFSREEALRLGHDYIGTEHLLLGLIREGEGM
                     AIKILKSLGINLQELRKSVESTVRGSTASINNLSNIPLTKQAEKALKITYLEAKVFRS
                     DVIGTEHLLLAILKDDDNIATQILNQYGISYDIVKEELEIIKSNFKSETPQSSSEDDP
                     YSKDDEGGGGSGSGMGGKKPSDSKSKTPVLDNFGRDLTKAAEEGRLDPIVGREKEIER
                     VSQILSRRKKNNPILIGEPGVGKSAIAEGLALRIVQRKCSRVLFNKRIVTLDLASLVA
                     GTKYRGQFEERMKAVMNELEKSPDVILFIDEIHTIIGAGGASGSLDASNMFKPALARG
                     EIQCIGATTLDEYRQYIEKDGALDRRFQRVLVEPASADETLEILNNIKERYEEHHNVN
                     FTPEALKACVTLTNRYITDRFLPDKAIDALDEAGSRVHITNIDVPKNILELEGKIADI
                     KNEKNRVVRSQKYEEAAKLRDTEKQLLEQLEVAKRQWEEETKSHRYTVSEQDVAEVVA
                     MMTGIPVTRVAQTESNRLIKMGELLKGSVIGQDEAIAKIVKAIQRNRAGLKDPNKPIG
                     TFVFLGPTGVGKTQLAKVIAKYLFDSEDALIRIDMSEYMEKFAVSRLIGAPPGYVGYE
                     EGGQLTEKVRRKPYSVVLLDEIEKAHPDIFNLLLQVLDEGMLTDSLGRKVDFKNTIMI
                     MTSNIGSRQLKDFGQGVGFQTTSKSSQADEHARGVVENALKKAFAPEFLNRIDDVVMF
                     NSLSKEDIHKIIDVELVHLYKRVQELGFKIVLSGDAKDYIADKGFDQNYGARPLKRAI
                     QKYLEDPLAEEIIKSEISEGDTIDVTFDKEKSELKIAVVKASTPPPPAKSGPGRKKKD
                     SE"
     gene            complement(3253..>3749)
                     /locus_tag="P5292_04730"
     CDS             complement(3253..>3749)
                     /locus_tag="P5292_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS38466.1"
                     /translation="YTESNYDYYLKGADVSALSLHKMLQAAMKLDAMNEWGSVVALTY
                     IAAQRTFPFYTDMADIKALLESIARSFGYHYGKAKKVRVNTVSQSPTKTTAGTGIKGF
                     DDFYNFADELSPLGNASADACAEYCVALFSDLTRMVTMQNLYHDGGYSTTGISQSIMD
                     RFGK"
BASE COUNT          984 a         1033 c          936 g          796 t
ORIGIN      
        1 tcgtaaacgg aaccatcgcc atgcggatgg tatttaccca atacttcccc tacgatacgg
       61 gcggatttct tatagggcct gttactcagt acacccagat caagcatgcc gtagagcacg
      121 cgacggtgca cgggtttcag gccgtcccgg acatccggca acgcacgcga tacgatcacc
      181 gacatcgagt aatcgatgta ggcggtcttc atttcctctt cgatgttgat cggaatgatc
      241 ttttctccgt cagtcatgat ggctaaactt caattcaaaa tttttcggct ggataaccgt
      301 ctcttccttt gtgtaaggtt ccggaaaagt cggtccgccg ggcctttccg atacccatcg
      361 gtagggccaa aatggcgctt ttactagaag cccgaaggta cgtaaaaatg ccgttccggg
      421 ggcgtttttc gcaaacagaa atttgttgat aaaagcaact gcccgattgg cagtccccaa
      481 aagctagcac gttacttgca aactaattcc gcaagaaaat cggtagtttt gttgaaagaa
      541 atcgatgatt cgaccgaact tgtctacagc cggttcgttt tacattagaa tcacaacccc
      601 aaacatggaa gccaaattct caccgcgtgt caaagatgta atcaccttca gtcgcgagga
      661 agcccttcgc cttggacacg attacattgg taccgaacac ctcctgctgg gcctcatccg
      721 cgagggtgaa ggcatggcca tcaaaatcct gaaatccctc ggcatcaacc tgcaggaatt
      781 gcggaaatcc gttgaaagca ctgttcgcgg cagcacggcc tcgatcaaca acctcagcaa
      841 tattcccttg accaagcagg ctgagaaggc actcaagatc acctatctcg aggccaaagt
      901 tttccgcagt gatgtcatag gaacagagca tctgctcctg gccatcctca aggacgacga
      961 taacatcgca acccagatcc tcaatcaata cggtatcagt tatgatatcg tgaaagagga
     1021 attggagatc atcaaatcca acttcaagtc ggagactccc cagagctcat cggaagatga
     1081 cccctactcc aaagacgatg aaggtggcgg tggcagcggt tcgggcatgg gtggtaagaa
     1141 accctccgac agcaaatcca aaacaccggt tcttgacaac ttcggtcgtg acctcaccaa
     1201 agctgccgaa gaaggtcgcc tcgacccgat cgtcggccgt gaaaaggaaa tcgaacgcgt
     1261 ctcgcagatc ctctcccgtc gtaagaagaa caacccgatc ctgatcggcg aacctggcgt
     1321 aggtaaatcc gccatcgccg aaggactcgc gctccgcatc gtccaacgga aatgttcacg
     1381 cgtcctcttc aacaagcgga tcgtgacgct cgacctggct tcgctcgttg ccggtaccaa
     1441 gtaccgtggc caattcgaag aacgcatgaa agcggtgatg aacgaactgg aaaaatctcc
     1501 ggatgtgatc ctgttcatcg acgagatcca caccatcatc ggtgccggtg gcgcctccgg
     1561 ttcactcgat gcttccaaca tgttcaagcc ggcgcttgcc cggggtgaga tccagtgcat
     1621 cggtgctact accctcgatg aataccgcca gtatattgaa aaagacggtg cgcttgaccg
     1681 tcgcttccag cgtgtcctgg ttgagccggc ttctgccgac gaaaccctgg agatcctgaa
     1741 caacatcaag gagcgctatg aggagcacca caacgtgaac ttcacgccgg aagccctcaa
     1801 ggcctgcgta acgctgacta accgctacat caccgatcgt ttcctgccgg acaaggccat
     1861 cgacgcgctc gacgaagcgg gatcacgcgt acacatcacg aacatcgacg tccccaagaa
     1921 cattctcgaa ctcgaaggca agatcgccga tatcaagaac gagaagaacc gtgtcgtccg
     1981 cagccagaaa tacgaagaag cagccaaact ccgggatacc gagaagcagt tgctggaaca
     2041 actggaggtg gccaaacgtc aatgggaaga agaaacaaag agtcaccgct acaccgtttc
     2101 cgaacaggat gtcgctgaag tagtggccat gatgaccggc attccggtca cccgtgtggc
     2161 ccagaccgag tcgaaccgcc tcattaagat gggtgaactg ctcaaaggca gtgtgatcgg
     2221 ccaggatgaa gcgatcgcca agatcgtgaa ggccatccag cgaaaccgcg ccggattgaa
     2281 agacccgaac aaaccgattg gaacgttcgt cttcctcggc cccaccggag ttggtaagac
     2341 ccaattggcc aaagtcatcg cgaaatacct gtttgactcg gaagacgcgt tgatccgcat
     2401 cgacatgagc gaatacatgg aaaagttcgc ggtatcccgc ctgatcggtg cgcctcctgg
     2461 atacgtcggt tatgaagaag gcggacagtt gaccgaaaag gtccgccgta agccttattc
     2521 ggttgtactc ctcgatgaga ttgaaaaggc ccatccggat atctttaacc tgctcctgca
     2581 ggtattggat gaaggtatgt tgaccgacag cctcggtcgc aaagtcgact tcaagaatac
     2641 catcatgatc atgacctcca acatcggttc ccgccagttg aaggacttcg gtcagggtgt
     2701 tggattccag acgacctcga aatccagtca ggccgacgaa cacgctcgcg gcgtagtcga
     2761 aaatgcgctg aaaaaggctt tcgctcccga attcctcaac cgtatcgacg atgtggtcat
     2821 gttcaactcc ttgtccaagg aggatatcca caagatcatt gatgtcgaac tcgtccacct
     2881 ctacaaacgt gtacaggaac tcggcttcaa gatcgttttg tcaggcgatg ccaaagacta
     2941 catcgccgac aaaggcttcg accaaaacta tggtgcccgc ccattgaagc gcgccatcca
     3001 gaaatacctg gaagatccgc tggccgaaga gatcatcaag tcagaaatca gtgaaggtga
     3061 tacgatcgat gtcacctttg acaaggagaa gagcgaattg aagatcgcgg ttgtgaaagc
     3121 ttccacgcct cctcctccgg ctaaatccgg ccccggtcgc aagaagaagg actcggaata
     3181 accgaaccga ttaattaaaa aaggctgccc gaaggcagcc ttttttaatt agatcaacaa
     3241 cagaattgct gtttatttcc caaagcggtc catgatcgac tgactgattc cggtggtcga
     3301 atatccaccg tcgtggtaga gattctgcat cgttaccatc cgggtcaggt cactgaacag
     3361 cgctacgcaa tactccgcac aggcatccgc actggcgtta cccagcggac tgagttcgtc
     3421 cgcgaaattg taaaaatcgt caaagccttt gatgccggta ccagccgtcg tcttcgttgg
     3481 cgactgggaa accgtgttga cgcggacctt ctttgctttt ccgtagtggt atccgaaact
     3541 gcgggcgata ctttccagca gtgctttgat atcggccata tcggtataga acggaaacgt
     3601 acgctgtgcc gcaatgtagg tcagcgccac gacgctaccc cattcattca tggcgtccag
     3661 cttcatggct gcctgcaaca tcttgtgtaa cgacaaagcg gacacatcgg cgcctttcag
     3721 gtagtagtcg taattgctct cggtatagg
//
LOCUS       DAOTON010000181         3745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19549_length_3745_cov_2.687263, whole genome shotgun sequence.
ACCESSION   DAOTON010000181 DAOTON010000000
VERSION     DAOTON010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19549_length_3745_cov_2.687263"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /gene="rny"
                     /locus_tag="P5292_04735"
     CDS             complement(<1..1387)
                     /gene="rny"
                     /locus_tag="P5292_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRS38467.1"
                     /translation="MDSQIIILIAEGLVAMIIGFLVSRYVMARTMKSKETAAESKAQS
                     IIKEAELEAEVIKKNKILEAKERFIQLKAEHEKSIGEKDKNIAAAENRIKQKESQLAQ
                     KVEQTQKKQQEYDTLQANLKNQLQILEGKKEELAKLHQRQVEQLEKISGLSVDQAKSQ
                     LVEALKAEAKTEAMSSIKDIMEEAKLTANKEAKRIVIQTIQRVATEHAVENAVTVFPI
                     ENDEVKGRVIGREGRNIRALEAATGVEIIVDDTPETIILSCFDPVRREIARLALHQLV
                     SDGRIHPARIEEVVAKVKRQLEEEIVEVGKRTTIDLGIHGLAPELIRMVGRMKYRSSY
                     GQNLLQHSREVANLCATMAAELGLNAKLAKRAGFLHDIGKVPDDEPELPHAILGMKLA
                     EKYKENPEVCNAIGAHHDEIEMNSLISPIVQVCDAISGSRPGARREVVESYIKRLKEM
                     EALALSYPGVEK"
     gene            complement(1746..2057)
                     /locus_tag="P5292_04740"
     CDS             complement(1746..2057)
                     /locus_tag="P5292_04740"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRS38468.1"
                     /translation="MGELLSVKVSIGNRTYPLRITREEHERVTQAAELINKRMKEFEE
                     LYAVKDKQDLLAMCALQFANESMGHHTASASSGKDEVGDRIEYLTLLVDEYLSKEAPS
                     Q"
     gene            complement(2069..2377)
                     /locus_tag="P5292_04745"
     CDS             complement(2069..2377)
                     /locus_tag="P5292_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38469.1"
                     /translation="MTQPNDVQHQVGELENRIHKFIHLHDTLRAQCQELLSENRRLQL
                     ELDEERAKSRRVEEGYRNLQDVEQQAARQRVAGIKRKIGDIIGEIDRNLTLIDVQQHK
                     "
     gene            2571..>3745
                     /locus_tag="P5292_04750"
     CDS             2571..>3745
                     /locus_tag="P5292_04750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRS38470.1"
                     /translation="MFRRLISATLLFLSASTCTHGQTDYPKNYFSPPLDSDLVVVGTF
                     GEIRPDHFHTGIDLGTGEMEGWPVKASADGWISRIKVDSRGFGKALYITHPNGFVTVY
                     GHLKSFEEPVASLVRKQQDKNEVYEVELFPKKNELKVKKGQIIALSGNTGNTSGPHLH
                     FEIRDEVTEQPINPLLFGWNVPDNIAPVLKNLRIFPVREAGMLDKCDTAITYEILPTE
                     GLYILNTADFIQASGSIAFGFETVDQIDGSAATLGIYTAEFYVDGQLAYQWRMDRLDF
                     DEQRYVNAHIDYLSKVRDGYTIERCHRLPGNHFSLIYSDTNATGYSIFGDDAAHDLKF
                     IARDFAGNASQIEFQVIAYSTLFNNPYLPRPEGSVLVSNQKGIAVHKAHLDVTIPNGA
                     "
BASE COUNT          779 a         1058 c          944 g          964 t
ORIGIN      
        1 tcttctctac tccggggtac gacaaggcca gtgcttccat ctctttgagt cgcttgatgt
       61 acgactcgac cacttcgcgt cgcgcccccg gacgggaacc ggaaatggcg tcacatacct
      121 ggacgatcgg cgagatcagg ctgttcattt cgatttcgtc gtggtgagcg ccgatggcgt
      181 tacagacctc cgggttctcc ttgtatttct cggcgagttt catgccgagg atcgcgtgcg
      241 gcaattccgg ttcgtcgtcc ggaactttgc cgatatcgtg caagaagccg gcacgcttgg
      301 ccagcttggc gttcaatccg agttccgccg ccatggttgc gcacaagttg gcgacttcac
      361 ggctgtgctg gagcaggttc tgaccgtagg aagaacggta tttcatgcgg cccaccatcc
      421 ggatcagttc cggggcaagt ccgtgaatac cgagatcgat ggtggttcgc ttaccaactt
      481 ccacgatctc ttcttccagt tgacgtttta ccttggcgac gacttcctcg atacgcgcgg
      541 ggtgaatccg gccatcggaa accagttggt gaagcgcgag gcgggcgatc tcccggcgta
      601 cggggtcgaa acaagagaga atgatcgtct ccggcgtatc gtcgacgatg atctccacac
      661 cggtagctgc ctccagggcc cggatgttcc ggccttcccg gccgatgaca cggcctttca
      721 cttcgtcgtt ctcaatcgga aataccgtaa cggcattctc cacggcgtgt tcggttgcaa
      781 cgcgctgaat ggtttgaatg acgatccgct ttgcttcttt attcgcggta agtttggcct
      841 cttccatgat gtccttgatg gacgacatgg cttccgtctt ggcctccgcc ttcagggctt
      901 cgacaagttg gcttttggcc tgatcaacac tcagtccact gatcttttcc agttgttcga
      961 cctggcgctg gtgcaacttg gccagttctt cttttttacc ctcaaggatc tgcaactggt
     1021 tcttcagatt ggcctggagc gtgtcgtact cctgttgctt cttctgggtc tgctcgacct
     1081 tttgggccag ctgcgattcc ttttgtttga tgcgattctc ggccgcagcg atgtttttat
     1141 ccttttcgcc gatggatttc tcgtgttcgg ccttaagttg aatgaatcgt tccttggcct
     1201 cgagaatctt gttcttcttg atgacttccg cttccagttc ggcttccttg atgatcgact
     1261 gggcctttga ttcggcggcg gtctccttcg acttcatggt tctggccatg acatatcgcg
     1321 aaaccagaaa cccgatgatc atggctacca gtccttcggc aatcagaatg atgatttggc
     1381 tatccatata ggtgcttact tatgttgagg cggcttatgc cgctggttga caattccacc
     1441 ggttacccga tggtcaataa aaaacccgca tctgtcccgg aactcataga aaaccccgcg
     1501 atacagggtt ggaacctcct ggtttctctg gtttctgctg tcccggagga ttcccgcgaa
     1561 gcgggagtgc agcctgccat actaaccact gagttgggct ccaatggtat tgaagtgttg
     1621 agtttttata agtgaatcgg aactgatgcg ggatattatc ccgactcggt gcaagggacg
     1681 atccggcgaa tcacttcgac aggacgaccg gttcacaccg attcgacttc aaagaacgat
     1741 accctttatt gggatggcgc ttctttggac agatattcgt ccaccaaaag cgtcaaatac
     1801 tcaatccggt cgccaacttc gtcttttcct gacgaggccg aggctgtatg atgacccatt
     1861 gattcattcg caaactgcaa tgcgcacatg gccagcaggt cctgcttatc cttcacggcg
     1921 tagagttctt cgaactcttt catccgctta ttgatcagct cggcagcctg ggtcacgcgc
     1981 tcgtgctcct cccgggtgat ccggagtgga taggtgcgat taccaatgga aactttgacc
     2041 gacagtaatt ctcccatgga gggttgattc acttatgctg ttggacatcg atcagggtaa
     2101 gattgcggtc aatctcgccg atgatgtcac cgatcttgcg tttaataccc gccacccgct
     2161 gacgtgccgc ttgttgctca acgtcctgca ggttccggta cccttcttcc acacgcctgc
     2221 tttttgcccg ctcttcgtcc aactccaact gcaaccgtcg attctcggac agcagttcct
     2281 gacattgtgc acgaagggta tcgtgcaaat gtatgaattt atggatgcgg ttctcgagct
     2341 cgccgacctg gtgctgaacg tcgttcggtt gggtcatgac aaacgtttct tggaagataa
     2401 gccccgaaag tacggattcc tcctgaatta tcacataaat tatgaacagc gattgttcag
     2461 aaaatcagcc agatgaggat gctcaggcaa agtaaagaaa tggggcagca aggcgtgatt
     2521 tcatctcatt tccatccctt cgaacgtcag gaattcccgt tctttgtttc atgttccgac
     2581 gcctcatttc ggccaccctg ctcttcttga gcgcctctac ctgcacgcac ggccagaccg
     2641 attacccgaa aaattacttc tctccgcccc tcgacagcga cctggtggtc gttgggacat
     2701 ttggagagat ccggcccgat cactttcata ctggcatcga tctcggaacc ggtgaaatgg
     2761 aaggatggcc ggttaaagct tccgccgatg gttggatctc caggatcaaa gtggatagcc
     2821 gcggctttgg taaagccctg tatatcaccc acccgaacgg ttttgtgacc gtttacgggc
     2881 atctaaaatc attcgaagag ccggtcgcct ccctggttcg aaagcaacag gacaagaacg
     2941 aagtctacga agtcgaactc ttcccaaaga agaatgaatt gaaggtaaag aaaggccaga
     3001 tcattgccct ctcgggaaat acagggaata cctccggccc ccatcttcac tttgagatcc
     3061 gcgacgaggt gaccgaacaa cccatcaacc ccctgctatt cggctggaat gttcctgaca
     3121 acatcgcgcc ggtcctcaag aatcttcgga tcttcccggt acgtgaagcg ggcatgctcg
     3181 acaaatgcga taccgccatc acctatgaaa tcctcccgac ggaaggattg tacatcctca
     3241 atacggccga cttcatccag gcatccggca gcatcgcatt cggtttcgag accgttgacc
     3301 agatcgacgg ttctgccgcg accctgggga tctacacagc cgagttctac gtagatggac
     3361 aactcgccta tcagtggcgg atggaccggc tcgactttga cgagcaacgg tacgtgaacg
     3421 cgcacatcga ctatctctcc aaagttcgcg atggttatac catcgaacgc tgccatcgcc
     3481 tgcccgggaa ccacttttcg ctcatctaca gcgacaccaa cgcgaccggt tacagcatct
     3541 ttggtgacga tgccgctcac gatctgaaat tcattgcacg cgacttcgcc ggtaacgctt
     3601 cgcagatcga gttccaggta atcgcctatt ctaccctctt caacaatcct tacctgcctc
     3661 gcccggaggg ctccgtcctt gtttccaatc agaaagggat cgccgttcac aaggcccatc
     3721 tggacgtgac gattccgaac ggtgc
//
LOCUS       DAOTON010000182         3740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19584_length_3740_cov_3.466214, whole genome shotgun sequence.
ACCESSION   DAOTON010000182 DAOTON010000000
VERSION     DAOTON010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19584_length_3740_cov_3.466214"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..706)
                     /locus_tag="P5292_04755"
     CDS             complement(86..706)
                     /locus_tag="P5292_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38471.1"
                     /translation="MKPHQPLLLSFALLLWVSVSAQKTLERVSERVCRRMEKANLQRP
                     ADSLRTEMMNIFMEEAMLDVDGFMKDYKLKTFDESAGQMIGQELGKHLVSTCPAFLQF
                     SLALAKDEVATEEQAASNEHEELYAYDHADETELRHLYFLDEKGKEVKFYWTEHFPGS
                     DLFTVNPERHKGKHFSVKWKDAEYFLPKADGYYQIRILSSIELREN"
     gene            complement(743..1759)
                     /gene="murB"
                     /locus_tag="P5292_04760"
     CDS             complement(743..1759)
                     /gene="murB"
                     /locus_tag="P5292_04760"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRS38472.1"
                     /translation="MQILQNTSLRGYNTFGLEVRADWFAEVRSVDDLIGLVRDPKFQP
                     MPKMILGGGSNVLFTGDVHALVIYNKILGKSLRPDGDDHFLVTAGGGETWHELVKYSI
                     EHSCGGIENLSLIPGSAGAAPIQNIGAYGVELKDHFHELEAIDLTSGDVRIFSKEDCA
                     FGYRDSVFKRSEKGKYLIASITLRLDRHHRVRTHYGAIEQELSKMGILNPGIRDVSEA
                     VIRIRSSKLPDPAVIGNAGSFFKNPEVSQEDYDRLHALFPDLIAYPGASGKMKLAAGW
                     LIEKAGWKGKRLGNVGMHEKQALVLVNYGGATGIELIEHARRVQQHVQDQFGVLLEME
                     VNIL"
     gene            complement(2041..2646)
                     /locus_tag="P5292_04765"
     CDS             complement(2041..2646)
                     /locus_tag="P5292_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38473.1"
                     /translation="MQRLKAFFLFFLLPLFAQSQTFVQGNVMVTAGAQLGIHQVVTSD
                     EIRGTRRLGGTVSRILPFGLEVAATDYLGICVHYRKDTYVSTRDSVDAENSEWNIGAN
                     VHFLRTQLTNVFVGLHGGPSKFKYTELTDLDEFEAKGLHLALVSGANMYFSNRFGLQF
                     SAAFNNLIYRDGVVKAKNGDSTRYDLFLTGVTLGLSLVFII"
     gene            2749..3324
                     /gene="lpcA"
                     /locus_tag="P5292_04770"
     CDS             2749..3324
                     /gene="lpcA"
                     /locus_tag="P5292_04770"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HRS38474.1"
                     /translation="MTNNDPIRQHLEEARQCLETFLKNEQNLECIRDAANLMLDAIRS
                     GGKILSCGNGGSLCDAMHFAEELSGRFREDRRALPAIAISDPSHLTCVANDFGYDEVF
                     ARYVSAIGRSGDVLLAISTSGNSANVLKAIAAARQHGMKVVGLTGKDGGKMAGMCDVE
                     IRAPHSAYADRAQEIHIKVIHCLIDAIERGI"
     gene            3418..>3740
                     /locus_tag="P5292_04775"
     CDS             3418..>3740
                     /locus_tag="P5292_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRS38475.1"
                     /translation="MLVKTYGSAVYGIHATTITVEVNLGTGINFYLVGLPDSAVKESH
                     KRIVAALRNCKFNNPRHEITVNMAPADIRKEGSAYDLPIALGILAGSAQVNQERLGEY
                     VIMGEL"
BASE COUNT          835 a          975 c         1038 g          892 t
ORIGIN      
        1 atgatacgct tcagatcctg attgaccgat tgggaaaaga acttgtcgaa tcggaagcgg
       61 tactaagaga ggtacgctga caacatcagt tttcccgtag ttcaattgaa ctcaggatcc
      121 gtatctggta ataaccatcc gccttgggta gaaagtactc cgcgtctttc catttcacgg
      181 aaaagtgttt gcccttgtga cgttcagggt tgaccgtgaa gagatcggat cccggaaagt
      241 gctccgtcca atagaatttc acttcttttc ccttctcgtc gaggaagtag agatgccgga
      301 gttcggtttc gtccgcgtgg tcgtaggcgt atagttcttc gtgttcgttc gaagcagcct
      361 gttcttccgt agcgacttca tctttcgcaa gcgccaggga aaactggagg aacgcgggac
      421 aagtcgaaac gaggtgtttg cccagttcct gcccgatcat ctgcccggcg gattcatcaa
      481 acgttttgag tttgtaatcc ttcatgaacc cgtccacgtc cagcatcgct tcttccatga
      541 agatgttcat catctccgtc cgcagcgaat cggcgggccg ttgcaggttg gctttttcca
      601 tccgacgaca aacgcgctcg gatacccgtt ccaatgtttt ttgagcgctt acgcttaccc
      661 ataacaagag ggcgaaggag aggaggagcg gttgatgtgg tttcatgata gagcgataac
      721 gaagataagt cattcttgct gcttaaagga tattcacttc catttccaga agtacgccaa
      781 actgatcctg aacatgctgc tggacccgcc gggcatgctc aatcagttct atcccggtcg
      841 caccgccata attgaccaac acgagcgcct gtttttcgtg catgccgacg ttcccgagac
      901 gcttaccctt ccatcccgct ttttctatca gccatcccgc ggccagtttc atttttccgg
      961 aagcgccggg gtaggcgatc aagtcgggaa atagagcgtg taatcggtca tagtcctcct
     1021 gactcacttc cgggttcttg aaaaagctgc cggcatttcc gattaccgcc ggatcgggca
     1081 gcttgctgct gcggatccgg atcacggctt cgctcacatc ccggatgccg ggattcagga
     1141 tacccatttt cgatagctct tgttcgatag cgccgtagtg tgtccggacg cggtggtgcc
     1201 ggtcgagtcg aagggtgatc gacgcgatca ggtactttcc tttttcactt cgtttgaata
     1261 cgctgtcgcg gtaaccgaat gcgcaatctt ctttgctgaa gatgcgtacg tccccgcttg
     1321 tcagatcaat cgcttcgagt tcatggaaat gatctttcaa ctcgacgccg tatgccccaa
     1381 tgttctggat gggcgctgcg ccggctgatc ccggaatgag ggagagattc tcgattccgc
     1441 cgcaggaatg ttcgatgctg tacttgacga gctcgtgcca ggtctcgcca ccaccggccg
     1501 tcaccaggaa atgatcatcg ccatccggcc gcaacgactt ccccaggatc ttgttgtaga
     1561 tgaccaatgc atggacatca ccggtgaaga gaacattgct tccgcctccc aggatcattt
     1621 tgggcatcgg ctggaacttc ggatcccgga ccagcccgat cagatcatcc accgatcgaa
     1681 cttccgcgaa ccagtcggct cgtacctcca ggccgaacgt attgtatccc cgaagggagg
     1741 tgttttgcag gatttgcatg gcgggaaagg gtacggacga aggtaatcct gaaggagcaa
     1801 atcaacccgt agcgtaaccg gttttttaaa cgcggttact cacattgaaa ttcgattcat
     1861 tcgccaagcc tgtaaggaag catgccgaat tatcttgccg tccggcacta ccggggatat
     1921 cttcgggaca cagtctcatg gttcaggtcc gctcagcgga ccgtggtttt tggttaaacg
     1981 accagaggtg taattcgagt taaggcacga cagcggcccg cacagccgct gttcgtgttt
     2041 ttatatgatg aacaccaggg agaggccaag cgtgacgcct gtgaggaaga gatcatatcg
     2101 ggtggagtcc ccgttcttgg cctttaccac accatcccga tagatcaggt tattgaaggc
     2161 cgcggagaat tgcaacccaa agcggttgga gaagtacata ttggcgccgc taaccagggc
     2221 gaggtgcagg ccttttgcct cgaattcgtc cagatcggtc agttccgtgt acttgaactt
     2281 ggatggtccg ccatgaagtc ccacaaaaac gttggtcagt tgagtgcgca ggaagtggac
     2341 attcgccccg atgttccatt cgctgttttc ggcatcaacg gaatcgcggg tggagacata
     2401 ggtgtctttc ctgtagtgca cacagatgcc gaggtagtct gtcgccgcca cttcgagtcc
     2461 gaacggcagg atacggctga ccgtaccgcc caggcggcgg gtgccgcgga tttcgtcgct
     2521 ggtgaccacc tgatggatgc ccaactgggc gccggctgtg accatcacat tcccctgtac
     2581 gaacgtctgg gattgggcaa acaacggcag cagaaagaaa agaaagaagg ctttgagccg
     2641 ttgcataaat gcagttgaag gaatgcgtga atctccgggc aagatagtaa tttccgaggc
     2701 cggtattcgt tctctttcta ccttcacgcc gcagaaacaa gctgtctcat gaccaacaac
     2761 gatccgatcc gccagcacct ggaagaagca aggcagtgcc tggaaacgtt cctgaaaaat
     2821 gaacagaatc tggaatgtat ccgggatgca gctaatctta tgctcgacgc gatccgatcc
     2881 ggaggaaaga ttctttcctg tggaaacggt ggtagtttgt gtgacgccat gcacttcgcc
     2941 gaagaactgt ccggccgatt tcgggaggac cgtcgcgcat taccagctat cgcgatctcg
     3001 gacccgtccc accttacgtg tgtggcaaat gattttggct atgatgaagt gttcgcccgg
     3061 tatgtcagcg ccatcggccg ttcgggggat gtgctgttgg ccatcagcac gagtggcaac
     3121 tctgccaatg ttctgaaagc cattgcggcc gcgcgacagc atggaatgaa ggtcgtaggg
     3181 ctgaccggaa aggacggcgg taagatggcc gggatgtgtg atgtggaaat acgtgcgccg
     3241 cacagcgcgt atgccgatcg ggcgcaggag attcacatca aggtgatcca ctgcctcatc
     3301 gacgcgatcg aacggggtat ctgacccggt tcctgtagtt taggattacc gccggagcta
     3361 ccggttttga ttcacgcttt tcagcgtgcg gaatgatatt gtctaatctc aaggagtatg
     3421 ctcgttaaaa cgtatgggag tgccgtctat ggtattcatg ccacgacgat caccgtggag
     3481 gtgaacctgg gtaccggaat caatttttac ctcgtagggt tgcccgatag tgcggtgaag
     3541 gaaagtcata aacgcatcgt cgcggctttg cgtaattgca agttcaacaa cccgcgacac
     3601 gagatcaccg tgaacatggc gccagcggat atccgcaagg aaggttctgc ttacgacctg
     3661 ccgatcgcac tggggatcct ggctggctcc gcacaggtca accaggagcg cttgggcgaa
     3721 tatgtgatca tgggggagct
//
LOCUS       DAOTON010000183         3735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19619_length_3735_cov_3.185054, whole genome shotgun sequence.
ACCESSION   DAOTON010000183 DAOTON010000000
VERSION     DAOTON010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19619_length_3735_cov_3.185054"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="P5292_04780"
     CDS             complement(<1..195)
                     /locus_tag="P5292_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38476.1"
                     /translation="MISVSSSNPVQLLMRSLVSLCLVLLSHPGFVHAQFMPGMGGGAT
                     KIYDGKISGTIIDSLNNKPVE"
     gene            357..>3735
                     /locus_tag="P5292_04785"
     CDS             357..>3735
                     /locus_tag="P5292_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38477.1"
                     /translation="MRRSRVRLILSLLLLLLAGGLGLHWWLVRHTEEILQELVRQESD
                     GKVALQVTKARLRYRAHRVSLENVRLFSRDTAEGHAAFDLHFKSVDLQLASFSDMIFR
                     GLLVVPVIEVSRPVIEVLPSGRAGQRISLSEELGDVYIYIQKTLNNLQVQNLHITDGT
                     FALASRQQHNSRISIFNIGLQVNNFRVDPGDPDSSRFFFSDDVQLTTGRESIRFPDSV
                     HVLHYKDLRLSTRSRIVELDSCIISGTSRDSIQGSFELQFERIRLVDVDFSTLYREGR
                     LRMDSLQCTLPVFRLDLVRRQSANAANTRAVLSRLASKLAGDLDIKHASLLRSKVSIQ
                     VRKGDKTLPFRTENDDFYIRGLRIKQDQDPAIAVDAFDFFLRDYISYTADSLYSIRFD
                     SIRIRDEALVLTNLRLQPAKPSRDGSVRTLNVPAFVLRDVDWSRLLLERELSANEAVL
                     QHPVIRIVNTRSPGTVSAQAKETVAALSNWVLLKNLKLEDASIDVENKSSGTHLQMNE
                     LVAQLIPGDILKAPSFENLEAGLRALNVATTRIETPALRVDLGGLNYSGNKNSWTAAS
                     VNFQSANGRFIGQCDRMLVRNLQLPDKQDDVLRIGALSWESGSLRLQESPEKGSGRKR
                     LAKLPALRIDSVRLQNTSLEGALRNEGSVSTFIQQAGGAGISISPNGDWSGSLTDLVG
                     SGGAWMRPGATVRWQSMRQEDGGTVRANGVSCAVNGAADSIFASIPALRFRMSASGEG
                     RELLHASFLEIPDAVLRFRHFPKIDSLSASKRQLWKLLIDTLQVHRAELDLESGDERK
                     LRLVAKAAQLRAEPLRFRSDGSLFFGSTDLQLEQWQVESDDGRKADGRSGRLKIQCDS
                     LSAGGNTRDGWTAAVRKLVLQHVGFFYKNRSRELREVMVDGLEANSLLLNPQLVRSPE
                     RWWPEQKRMELHNASFHWHLKESELSLANAAWSAATKHFTAEDVSFRPYLSPDSFLSA
                     RAFETDYLRLHRGRLELEELDLDFLEETRFRSRKATVSDAFLEVRRDKRLPDPIGQLK
                     PLPVQQLMRLGFDFRIDTFAVTNGHVEYTEIGDKSGQPGTVSFDRLQATLTGIRSRDV
                     ADGDSLRLKANARFLDTTDIALRFRESYLDTLGGFVLT"
BASE COUNT          844 a         1038 c         1040 g          813 t
ORIGIN      
        1 ttcgaccggt ttgttgttga gcgagtcgat gatcgtgccg ctgatctttc cgtcatagat
       61 cttcgtcgcg ccgccgccca tgccgggcat gaactgagca tggacgaaac ccggatgaga
      121 gagcaatacg agacacagac tgaccagaga acgcatcaat aactgaacgg gattggagga
      181 ggaaacagag atcataagga gtgggcaggg gtcgctaaag gtacctccac tacctgtctg
      241 acactactgt gacgatgtta ttttgtgtta aaaaagcatg ttttaggctt ctgaatgtcg
      301 aaaaattgat gaaatcgcct gcacggggca ggtaatccgg tctatcttga aagctgatgc
      361 gccgttcccg ggtccgcctg atcctttccc ttctattgct gctgctggcc ggcggactgg
      421 ggcttcattg gtggctggtc cgacatacgg aagagatcct gcaagagttg gttcgacagg
      481 aatcggatgg caaagttgcc ttgcaggtaa caaaagcgcg cttgcgctac cgggcacacc
      541 gggtgagtct tgaaaatgtc cggctctttt cccgcgatac agcggaaggg catgccgcgt
      601 tcgatttaca tttcaagtcg gtcgatctgc aactagcctc cttcagcgac atgatctttc
      661 gcgggctgct tgtagttccg gtcatcgaag tcagtcgacc cgttatcgag gtgctgccat
      721 ccggacgtgc cgggcaacga atttcattga gtgaagaatt gggtgatgtt tacatctaca
      781 ttcagaaaac gctgaacaac ctgcaggtac agaatctgca catcaccgac gggaccttcg
      841 cgctggcatc gcggcaacag cacaacagtc gcatttccat ctttaacatc ggattacagg
      901 taaacaactt ccgggtcgat cccggcgatc cggattcttc acgcttcttt ttcagcgatg
      961 atgtccaact gacaaccggt cgcgaatcga tccgctttcc tgacagcgtg cacgttcttc
     1021 actacaaaga tctgcgcttg tccacccgga gccggatcgt cgagctggac agttgcatca
     1081 tttccggaac aagccgcgac tcgatccagg gatcattcga attgcaattc gaacgcatcc
     1141 gactggtgga tgtcgacttt tccaccttgt atcgtgaggg tcgcctgcgc atggattcgc
     1201 tgcaatgcac cttaccggta ttccggctcg atcttgttcg ccggcaatcc gccaacgcgg
     1261 ccaataccag agccgtactt tcccgtcttg cttcaaaact tgcaggcgac ctcgacataa
     1321 agcacgcttc gttgctgcgc agcaaggtgt cgatccaggt gcgcaaagga gataagacgc
     1381 tgccctttcg tacggagaat gacgacttct atatccgcgg actgcgcatc aaacaagacc
     1441 aggatcccgc gatcgcagtc gatgcgtttg atttctttct gcgcgactac atcagctaca
     1501 cggccgacag cctgtactcc atacgcttcg acagtatccg catacgggac gaagcgttgg
     1561 tgctgaccaa cctgcggttg caacccgcca aaccttcccg ggacggttcc gtccggacgc
     1621 tcaatgtacc ggccttcgtg ctgcgggatg tcgactggtc gcgcctgcta ctcgaacggg
     1681 agctttccgc gaacgaagcc gtactccagc atccggtgat ccggatcgtg aacacccgat
     1741 cgccgggaac cgtttccgct caggcgaaag aaaccgtcgc cgcgctcagc aattgggtct
     1801 tgctgaagaa cctgaaactc gaagacgcca gcattgatgt cgaaaataaa agcagcggca
     1861 cgcatttaca gatgaacgag ctggtcgcgc aattgattcc cggcgatatc ctgaaagcgc
     1921 cttcattcga gaacctggaa gcaggtttgc gcgctttgaa cgtcgccacc acccgcatcg
     1981 aaacgcctgc actccgcgtg gacctgggcg gcctcaacta ttccggcaac aagaacagtt
     2041 ggaccgccgc atcggtcaac tttcagtcgg ccaacgggcg attcatcggg cagtgtgacc
     2101 ggatgctggt ccggaattta caattaccgg acaagcagga tgacgtgctt cggatcggtg
     2161 cgctatcgtg ggaatccgga agcctgaggc tgcaggagag ccctgaaaaa ggaagcggtc
     2221 ggaagcggct tgcgaaactc ccggccttgc gcatcgactc ggtccgactt caaaacacca
     2281 gtctggaagg agcattgcgg aacgaaggtt ccgtgagcac ttttattcaa caggcaggag
     2341 gagccggcat ttcaatttcg ccgaacggag actggagcgg ttccttaacg gacttggttg
     2401 gaagcggtgg ggcctggatg cgaccgggcg caacggtccg atggcaatcg atgcgtcagg
     2461 aagatggcgg aacggtgcgc gccaacggcg tcagctgtgc tgtgaatgga gccgcggatt
     2521 cgatcttcgc atccattccg gcacttcggt tccgcatgag cgcctccgga gaaggtcggg
     2581 aattattgca tgcctccttt cttgaaatcc cggatgccgt actgcgcttt cggcattttc
     2641 caaaaatcga ttcactcagc gcgtcaaaac ggcaactatg gaaactgctg atcgacaccc
     2701 tgcaggtcca ccgcgccgaa cttgacctgg agagcggcga cgaacgaaaa ctgcgactgg
     2761 ttgcaaaggc cgcacaacta cgggccgagc cgctgcgctt tcgaagcgac ggatcccttt
     2821 tcttcggctc cactgattta caactcgaac aatggcaggt ggaatccgat gacggccgga
     2881 aagctgacgg ccgctctggg cgcttgaaaa ttcaatgcga ttccctgagc gcgggcggta
     2941 acacgaggga tggatggacc gcggccgtga gaaaacttgt gctgcaacat gtaggtttct
     3001 tctataaaaa ccgctcacgc gaattgcggg aagtgatggt ggatggattg gaagcaaaca
     3061 gcctgttgtt aaacccacag ttggttcgct ctccggaacg ttggtggccg gaacaaaagc
     3121 gaatggaatt gcacaacgct tcgtttcatt ggcatcttaa agaatccgag ttgagcctcg
     3181 ccaacgctgc ctggtccgcg gctacgaagc atttcacagc agaggatgtt tccttccgac
     3241 cgtacttgtc cccggatagc ttcctctccg caagggcatt cgaaacggat taccttcgac
     3301 tgcaccgcgg ccggctggag ttggaagaac tggatctcga cttcctggaa gaaacacggt
     3361 ttcgttcccg aaaagccacc gtttccgacg cgttcctgga agtccggcgc gacaagcgtc
     3421 ttccggatcc gatcggacag ttgaaaccat tgccggttca acagctcatg cgtcttggat
     3481 tcgattttcg catcgatacg ttcgccgtca cgaacggcca tgtcgaatac acggagatcg
     3541 gtgacaaatc agggcagccc ggcaccgtat cgtttgaccg gctacaggca acacttaccg
     3601 gcatacgaag tcgtgatgtg gctgatggcg attccctccg gctgaaggcg aatgcccggt
     3661 tcctggatac aaccgatatc gcgctgcggt tccgcgaatc ctacctggat acgctgggcg
     3721 gattcgtcct caccg
//
LOCUS       DAOTON010000184         3725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19686_length_3725_cov_3.991281, whole genome shotgun sequence.
ACCESSION   DAOTON010000184 DAOTON010000000
VERSION     DAOTON010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19686_length_3725_cov_3.991281"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="P5292_04790"
     CDS             complement(<1..1079)
                     /locus_tag="P5292_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRS38478.1"
                     /translation="MKRLSFFIAVLAVVFNSCRRNQVSLVQHNFGEEVEQQQNLVFTF
                     DKDLAPDSLQNVWDSTEYVRFEPKVDGKFRWNNARELVFSPSTGFAPSTDYKATITDA
                     VLKLNGQYGSVGEPKSMGFHSPYLKMTGANGYWVVSDMQPGRAALSIALDFNYPVDPT
                     AVSSLLKLKLGEREVSYQLRTQAPSAGLELLVDGIERSSMSDLSLSLLLQKGLPIHQG
                     TFKTKDDQQLSIAIPNPDELEITQAIPETEGMESRIRVFTTQAIDPESISQSVSVEPQ
                     VPFTASATQGGFLLNGEFQSGTAYTLTVSTELRGMLGGSLKEEYKSTVTMGEIEPAIA
                     FMSQKGVYLSSAGSRNIGVRLSSIP"
     gene            complement(1278..3023)
                     /gene="aspS"
                     /locus_tag="P5292_04795"
     CDS             complement(1278..3023)
                     /gene="aspS"
                     /locus_tag="P5292_04795"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRS38479.1"
                     /translation="MLRTHTCGELDLKAVGKTVTLAGWLQRSRDLGGMTFIDLRDRYG
                     ITQLVFNMEQDAALCEAARKLGREFVLQVSGQVTERENKNPKMATGDVEIIVREFKVL
                     NSAITPPFTIEDHTDGGDDLRMKYRYLDIRRNPVKEALLLRARVAAETRNYLNRQGFV
                     EVETPVLIKSTPEGARDFVVPSRMNQGEFYALPQSPQTFKQLLMVAGMDKYYQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFIEREDILSTFEGMARYLFQSVKGVDIGTLPRMTYAE
                     AMERYGNDKPDTRFGMELKDLNKLVKGKNFKVFDEAGLVVAIAVPGCAEYTRKQLDEL
                     TDWVKRPQIGMSGLVYAKYATDCTIKSSVDKFYSADDLKQWAVATDAQPGDLILVLAG
                     AEPKTRKAMSELRLEMGERLGLRDRNTFRCLWVVDFPLLEWGEESNRWHAMHHPFTAP
                     ISEDIGLLQTDPGKVRANAYDMVINGVEVGGGSIRIFDKQLQSRMFEVLGFSKDEAQH
                     QFGFLLNAFEYGAPPHGGIAFGFDRLCSLFGGADSIRDYIAFPKNNAGRDVMIDSPST
                     ISEEQLKELGIAIRA"
     gene            complement(3127..3711)
                     /locus_tag="P5292_04800"
     CDS             complement(3127..3711)
                     /locus_tag="P5292_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRS38480.1"
                     /translation="MKKTFVKVLVLVLLAVAGATVAQAQIKYGPMLGANLANIKGDFD
                     DDNAMKFGMHIGGVVDIGISDNFSIMPGVLFSMKGTQSDEDSKYKLNANYIEVPVLAK
                     YQLESGLNFAAGPYLGLLMSAKATDGDNDVDVKDSFSGTDIGLKFGIGYQLESGLGFG
                     VNYGMSLTTIADDSDIDSKNNVIGISVMYMLGGD"
BASE COUNT          843 a         1037 c          995 g          850 t
ORIGIN      
        1 ttgggaatcg aggaaaggcg cacaccgatg ttccggctgc cggcggaaga aagataaacg
       61 cccttctggc tcatgaacgc gatcgccggt tcgatctcac ccatggttac agttgacttg
      121 tattcctcct tcaagcttcc gcccagcata ccgcgcagtt ccgtggatac agtaagcgtg
      181 taggccgtac ccgactgaaa ctccccgttc agcagaaagc ccccctgcgt tgccgaggca
      241 gtgaacggaa cttgtggttc tacgctcacg ctttggctga tcgattccgg gtcaatggcc
      301 tgggtagtaa acaccctgat gcgcgactcc ataccctccg tctcgggaat cgcctgggtg
      361 atctccagtt catccggatt ggggatcgcg atactgagct gctgatcgtc ctttgttttg
      421 aaagtgccct gatgaatcgg aagccctttc tgtaacaaca ggctcaagct gaggtcactc
      481 atggaggaac gttctattcc atcgaccagt aattccaaac cggcggaagg cgcctgtgtg
      541 cgcaattggt acgaaacctc ccgctcgccc aatttcaatt tgagtaaaga agatactgcc
      601 gtcgggtcga ccggataatt gaaatccagg gcgatcgaaa gcgcggctct tccaggctgc
      661 atatcggata ccacccaata accgttagcc ccggtcatct tcaggtaagg cgaatgaaat
      721 cccatgctct tcggttcacc caccgaacca tactggccgt tcaactttaa aacggcgtct
      781 gtaatcgtag ccttgtaatc ggtactgggc gcgaagcccg ttgaaggaga gaagaccagt
      841 tcgcgtgcat tgttccagcg gaacttcccg tccaccttcg gctcaaaccg tacatactcc
      901 gtcgagtccc agacattttg cagagaatcg ggagcgaggt ccttatcgaa ggtgaaaacc
      961 aggttctgtt gctgttcgac ttcctcgccg aagttgtgtt gcaccaggct gacctggtta
     1021 cgacggcatg aattgaaaac tacggccaat acggcgatga aaaacgagag gcgtttcatg
     1081 ggaagatgtg gataggctgg cgggaagtgt ccttaaatgt agaactatga gagaaatgcg
     1141 gaaagcaggg caggctatta atcgaaaaat tccagaaggg ctcgaagatg gtcgtaaata
     1201 tccgggaata ataagccgga aacgaaacgg ccggtcacaa attggaccgg ccgttgaatt
     1261 ttacaccctg ccagggatca agcacgaatg gcgatcccaa gctccttcaa ctgctcctcg
     1321 ctgatggtcg aaggggaatc gatcatgacg tcgcgaccgg cattgttctt cggaaacgcg
     1381 atgtagtcgc ggatgctgtc ggctccgcca aaaagggagc aaagccggtc gaagccgaat
     1441 gcgatacctc cgtgaggcgg ggctccgtac tcgaaagcgt tcagcaggaa tccgaactgg
     1501 tgctgtgcct cgtcttttga aaagccgagc acttcgaaca tgcgggattg taattgtttg
     1561 tcaaagatcc ggatcgaacc tccgcctacc tccacgccgt tgatcaccat atcgtatgca
     1621 ttcgcccgga cctttccagg atcggtttgc agcagcccga tgtcttccga gatgggcgcg
     1681 gtgaaggggt gatgcatcgc gtgccagcgg ttgctctctt ctccccactc gagcagcggg
     1741 aagtcgacca cccataggca acggaaggtg ttacggtcgc gaaggcctaa gcgctcgccc
     1801 atctccaggc gtaactcgct catggccttc cgggttttcg gttccgcgcc ggccaacacc
     1861 aggatcaggt caccgggttg agcatcggtt gcaacagccc attgtttcag atcatccgcg
     1921 gaatagaact tatccaccga ggatttgatg gtgcaatccg tagcatactt cgcatagaca
     1981 agtccgctca tgccgatctg cggacgcttc acccaatcgg tcagttcgtc gagttgttta
     2041 cgggtgtatt cggcgcaacc gggaacggcg atcgcgacaa ccaaccctgc ctcgtcgaac
     2101 actttgaaat tctttccctt caccaatttg ttgaggtcct tcaactccat cccgaagcgc
     2161 gtatcgggct tgtcgtttcc ataacgctcc atcgcttcgg cgtacgtcat tcttggcaag
     2221 gtgccgatgt cgacaccctt caccgactgg aacaggtagc gcgccatgcc ttcgaaagtt
     2281 gaaaggatat cctcccgctc gatgaaactc atctcgcaat cgatctgcgt gaactccggc
     2341 tgacgatcgg cgcgcagatc ctcgtcgcga aagcatttta cgatctgata atatttgtcc
     2401 atgccggcca ccatcaacag ttgcttgaag gtctgcggtg attgcggaag cgcgtagaac
     2461 tcgccctgat tcatgcgcga gggcaccacg aagtcgcggg cgccttcggg agtcgacttg
     2521 atcagcaccg gtgtctccac ctcgacgaac ccttgccggt tcaggtagtt tcgcgtttcg
     2581 gcggctacac gggcgcgcag caacagcgcc tctttcaccg ggttacggcg gatgtcgagg
     2641 tagcggtatt tcatccggag gtcgtcgcct ccgtcggtat ggtcttcgat cgtaaacggg
     2701 ggtgtgatcg cgctattgag gactttgaat tcgcgcacga tgatctcaac atcgccggtt
     2761 gccattttcg ggttcttgtt ctcccgttcg gtaacctgcc cgctcacctg caagacgaat
     2821 tcacgaccca gctttcgggc agcctcgcag agcgctgcgt cctgttccat gttgaacacc
     2881 aattgggtaa tgccataacg gtcgcgcagg tcgatgaacg tcatcccgcc cagatcacgg
     2941 gatcgttgca gccatccggc cagggtaacg gtcttcccga ccgctttcag gtcgagctct
     3001 ccgcaggtgt gggtacgtaa catggaaatc gatggatagg aatgcgaagg tacaaaatgc
     3061 gcagggctaa aacaggaacg ccgccccttc cgggacggcg ttctaataat atcatgggat
     3121 caacgatcag tcaccgccaa gcatgtacat gaccgaaatt ccgatcacat tgttcttgga
     3181 gtcgatgtcg ctgtcgtcgg cgatggtggt cagactcatg ccgtaattga caccgaagcc
     3241 gaggccgctt tccaactggt agccgatgcc gaatttcaaa ccgatatccg taccggagaa
     3301 ggaatcctta acatccacat cgttgtcgcc atcggtagcc tttgccgaca tcaacaaacc
     3361 gaggtaagga cctgcagcga agttgaggcc gctctccaat tgatatttgg caagtaccgg
     3421 aacctcgatg taattagcat tcaacttgta tttgctatcc tcatcgctct gggtaccctt
     3481 catcgagaag agcacaccgg gcatgatgga gaagttgtcg ctgataccga tgtcgacaac
     3541 accgccgatg tgcatgccga acttcatggc attgtcatca tcgaagtccc ctttgatatt
     3601 cgccagattg gcacccagca tcggaccata tttgatctgc gcttgggcaa cggtcgcacc
     3661 cgccacggcc aggagcacga ggacgagtac tttcacaaaa gtctttttca tgatagtggt
     3721 ttttg
//
LOCUS       DAOTON010000185         3723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19702_length_3723_cov_2.951200, whole genome shotgun sequence.
ACCESSION   DAOTON010000185 DAOTON010000000
VERSION     DAOTON010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19702_length_3723_cov_2.951200"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="P5292_04805"
     CDS             <1..168
                     /locus_tag="P5292_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HRS38481.1"
                     /translation="KVTDYYQPSAEAGIRFDDPELGIPWRTPAPIVSSKDRILPSFRE
                     FMAQHPDAFGT"
     gene            299..1381
                     /locus_tag="P5292_04810"
     CDS             299..1381
                     /locus_tag="P5292_04810"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRS38482.1"
                     /translation="MASEHQYLITVYGTYFIASILFSFLINRLFLKFVRTLGIRNQDD
                     TVIRWGSQSKPAVGGFSFYILFLLSIITYSIFFDSNQVFLNKEFIGLLLASMLGFLLG
                     LADDAYNTKPFLKFLTQVTCAVILMVTGTEISLSYVEPVNYVITIVWVVGIMNSLNML
                     DNMDAITSTVSISIILSAMVVMIFHQEVGSVYFMVLTGVMAALIGFLYFNWNPSKMYM
                     GDTGSQFLGVFLAATGIKYLWNAEPPSGDLISARNLLLPLIVFIMPIIDTTTVVINRL
                     GRGKSPFIGGKDHTTHALAYLGLTDRQVALVFWGMTLVSLLLVVIIERFLVSWTHLYT
                     TLFSVYLIVMFGVFFYATRTVERNLR"
     gene            complement(1378..1971)
                     /gene="rdgB"
                     /locus_tag="P5292_04815"
     CDS             complement(1378..1971)
                     /gene="rdgB"
                     /locus_tag="P5292_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRS38483.1"
                     /translation="MKLVFATQNSNKIKEVRSLLPSSIQLISLRDLETGEELPETGRT
                     LEANASQKARYIYDKFGLNCFADDTGLEVEALGGRPGVWSARYAGPACRTEDNIRKVL
                     TELKDVTNRAARFRTVIALIVDGEVYLYEGIVNGRISLEPSGTGGFGYDPVFIPDQES
                     RTFAEMSLEEKNRISHRGMAIQAFISGLTTIVKERWR"
     gene            2068..3180
                     /gene="gmd"
                     /locus_tag="P5292_04820"
     CDS             2068..3180
                     /gene="gmd"
                     /locus_tag="P5292_04820"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRS38484.1"
                     /translation="MKVALITGITGQDGAYLAELLLQKGYMVHGIKRRSSLFNTDRID
                     HLYADPHEQNVRFKLHYGDLTDSTNLIRIIQEVQPDEIYNLGAMSHVMVSFETPEYTA
                     NADGVGTLRILEAIRLLGLTRKTKVYQASTSELYGLVQEIPQSERTPFYPRSPYAVAK
                     LYAYWITVNYREAYGMYAVNGILFNHESPLRGETFVSRKITRGVARIALGLQQQLFLG
                     NLDAKRDWGHAKDYVEAMWLMLQQEHPEDYVIATGTTTPVREFLKLAFEEVGISVEFT
                     GSGSEERAYVTRCDHPEYQLKKGQLVMSVDPRYFRPTEVELLLGDATKARKNLGWVPK
                     YDLRGLVKEMMAADVELFRKDKYLKEGGHKVFNYHE"
     gene            3173..>3723
                     /locus_tag="P5292_04825"
     CDS             3173..>3723
                     /locus_tag="P5292_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRS38485.1"
                     /translation="MNKHDRIYIAGHNGMVGSAIVRALEASGYRNLLYRSSSELDLRD
                     QAATKTFFEQERPDYVFLAAAKVGGIHANNIYRAEFLYDNLMIEANVIDAAWKFGVKK
                     LLFLGSSCIYPKNSPQPLKEEYLLSGYLEQTNEPYAIAKIAGIKLCESYRKQYGANFI
                     AAMPTNLYGPNDNYDLQQSHVLPA"
BASE COUNT          895 a         1012 c          878 g          938 t
ORIGIN      
        1 aaggtaaccg actactacca gccttcggcc gaagcgggga tccgtttcga cgaccccgaa
       61 cttggcattc catggagaac acctgcgccg atcgtttcat ccaaagaccg gatactcccc
      121 tctttccggg aattcatggc ccagcatccc gatgcattcg ggacctgacc cataacactg
      181 ccagcttcac ccacaaacgg cgctcgttta cattcactta ttctactttg acaaaaaggt
      241 cgaacctcat tataccaagc ccgctacctt cagcatcgat ttaaccgacc ggcattccat
      301 ggcatccgag caccaatatc tcatcaccgt ctacggtacc tatttcatcg cctccatact
      361 cttttcattc ctgatcaacc ggttgtttct caaatttgtc cgaacactgg gcatacggaa
      421 tcaggatgac acggtcatcc ggtggggttc ccaaagcaaa ccggcagtcg gcggcttctc
      481 cttctacatc ctatttctcc tctccatcat cacgtattcc atcttcttcg actcgaacca
      541 ggtcttcctc aacaaagaat tcataggcct attgctggcc tccatgctgg gattcctact
      601 tggactggct gatgacgcgt acaacaccaa gccatttctc aagttcctca cacaggtcac
      661 ctgcgcggtg atcctcatgg tgacgggtac ggaaatatcc ctttcttacg tggaaccggt
      721 caactatgtc atcaccatcg tgtgggttgt cgggatcatg aattccctga acatgctgga
      781 taatatggac gccatcacat cgactgtgtc catctcgatc atattatccg ccatggtggt
      841 gatgatcttc caccaggaag tcggaagcgt gtatttcatg gtgctgaccg gggtgatggc
      901 cgcactcatc ggcttcctgt atttcaattg gaacccgtca aagatgtaca tgggagatac
      961 cggaagtcag tttctcggcg ttttccttgc cgccaccggc atcaaatacc tttggaatgc
     1021 cgaaccgcca tccggcgacc tcatttccgc acgtaatctg ttgctgccac tcatcgtatt
     1081 cattatgccg atcattgata cgaccacagt cgtgatcaac cgacttggaa gggggaaatc
     1141 tccatttatc ggcggcaagg atcatacgac gcatgcgctt gcttacctgg gtttaaccga
     1201 tcgtcaggta gcgttggtat tttggggaat gacgcttgtt tccctcttat tggtggtgat
     1261 catcgaacgg ttcctggttt cctggacgca tctctacacc acgcttttct ccgtctacct
     1321 gattgtcatg ttcggcgtat ttttttacgc aacgcgtacg gtggagcgaa acctccgtta
     1381 acgccaacgt tccttcacaa tcgttgtcaa gccgctgatg aaggcctgaa tggccatgcc
     1441 gcggtgtgaa atgcggttct tctcttcaag cgacatttcc gcgaacgttc tgctttcctg
     1501 atcaggaata aagacgggat catagccgaa tccgccggtg ccggatggct caagtgaaat
     1561 ccgaccattc acaatccctt cgtagagata tacctctcca tccacgatca aggcgatcac
     1621 agtccggaag cgagctgccc ggttggtaac atctttcaac tcggtcagca ctttccgtat
     1681 gttgtcctcc gtacgacaag ccggaccagc ataacgagcc gaccatacac cgggtctgcc
     1741 tccaagggct tccacttcca acccggtatc atcggcaaaa caattcaagc cgaatttgtc
     1801 atagatgtaa cgggcttttt gtgacgcatt ggcttcgagc gttcgcccgg tttccgggag
     1861 ttcttcgcct gtttcgagat cgcgcagcga aatcagttga atgctcgatg gcaggagcga
     1921 tcgcacctcc ttgattttat tggaattctg agtggcaaag accagtttca tacgcacgta
     1981 ggcatccgga taaaagtcga aattcgcaat aattccgtat ctttgaaact atcggcccct
     2041 tacgggcgac ttgtctcaac tccttccatg aaagttgcgc ttattaccgg tattactggg
     2101 caagacggag cctaccttgc tgaacttttg ttgcaaaagg gctatatggt acacggcatc
     2161 aagcgccgta gttccctctt taataccgac cggatcgatc acctttacgc ggatcctcac
     2221 gaacagaacg tgaggttcaa acttcattac ggagacctta ccgatagcac gaacctgatt
     2281 cggatcatcc aggaagtgca accggacgag atctacaacc ttggagccat gtctcacgtg
     2341 atggtgagct ttgaaactcc tgaatacacc gcgaacgcag acggggtcgg aacgcttcgg
     2401 attttagaag ccattcgatt actcggtttg accaggaaaa cgaaggtcta ccaggcttcc
     2461 acttccgaat tgtacggcct ggttcaggag attccgcaat cggagcgtac gcctttctac
     2521 ccgcgctcac cctatgctgt cgctaaattg tatgcctatt ggattacggt caattatcgt
     2581 gaagcctatg gcatgtatgc tgtcaacgga attctgttca atcatgaatc accgcttcgc
     2641 ggcgaaacct tcgtctcgcg aaagatcact cgcggcgttg cccggatcgc actcggatta
     2701 caacagcaat tatttctcgg gaatcttgac gcgaagcggg attggggaca tgcaaaggac
     2761 tacgttgaag ccatgtggct gatgttgcaa caggagcatc ccgaagatta cgtcattgca
     2821 acaggtacta cgacacctgt gcgggagttc ttgaagctgg ccttcgaaga agtggggata
     2881 tcggttgaat tcaccggcag cggaagtgaa gaacgcgcct acgtcacacg ctgcgatcac
     2941 ccggaatatc aattaaaaaa aggtcagttg gtcatgtcgg tcgatccgcg ttactttcgt
     3001 ccgaccgagg ttgaattgct attgggagat gcgacaaaag cccgaaaaaa tctcggttgg
     3061 gttccgaaat acgacctgcg cggactcgtg aaagaaatga tggcggccga tgttgagtta
     3121 ttcagaaagg acaagtacct gaaagaaggc gggcataagg ttttcaatta tcatgaataa
     3181 gcacgacagg atttatatcg ccggtcataa cggtatggtc ggctccgcca ttgtccgtgc
     3241 cttagaggct tccgggtacc gcaacctctt gtatcgtagc tcatcggaac ttgatttgcg
     3301 agatcaggct gcaacgaaaa cgttttttga acaggagcgg ccagactatg tttttctcgc
     3361 agcggccaag gtgggtggga tccacgccaa caacatctat cgggccgaat tcctctacga
     3421 taatctgatg atcgaggcca atgtgatcga tgccgcctgg aaattcggcg tcaagaagtt
     3481 gttgttcctc ggttcttcct gcatctatcc caagaactcc ccacaacctc tcaaagagga
     3541 atacctgctt tcgggctatc tggagcaaac gaacgaaccc tatgcgatcg ccaagattgc
     3601 cggcatcaag ctttgcgaaa gttatcgtaa acaatatggc gcgaatttca ttgcagcaat
     3661 gccgaccaac ctttacggcc ccaacgacaa ctacgatttg cagcaatccc acgtattacc
     3721 cgc
//
LOCUS       DAOTON010000186         3708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19784_length_3708_cov_3.413359, whole genome shotgun sequence.
ACCESSION   DAOTON010000186 DAOTON010000000
VERSION     DAOTON010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19784_length_3708_cov_3.413359"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="P5292_04830"
     CDS             complement(<1..1217)
                     /locus_tag="P5292_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005986971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRS38486.1"
                     /translation="MKKVLCVLVSLIGLTVPSMSQVTLTPFASGFTSPVDIKNCGDGR
                     IFVLEQNGQINILDTAGNINPVPFLDIVGRVLYSGEQGCLGLAFPPDFATSQVFYVNY
                     TRRPDGDTRISRFHVSATNPDSADANSEEILLTIHQPYSNHNGGHIQFGKDGYLYIGM
                     GDGGSGGDPENRAQNPDSLLGKMLRIAVDPSNPTYSIPPTNPFAGNPSLGAEEIWALG
                     VRNPWRWSFDRATGDLWIGDVGQGTYEEIDYEPYGSGGGLNYGWRCYEANASYNTAGC
                     LAQSNYVSPVHFYPRSSGYSVTGGYVYRGARYADIYGKYFFSDYGVSSIRTLEPDGAG
                     GFTHTNLGSLGASAIVGFGEDKWGELYVAALGGTVYRLRSTACQPAATINAGSDTLYD
                     CGTGSVTLNAPLGA"
     gene            complement(1332..1508)
                     /locus_tag="P5292_04835"
     CDS             complement(1332..1508)
                     /locus_tag="P5292_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38487.1"
                     /translation="MKNEPVVTYNHMEEDDAGRTVYPEAQAKESLPISLYMLLVVDAI
                     LVSAIVFLTFKVIF"
     gene            1698..3377
                     /locus_tag="P5292_04840"
     CDS             1698..3377
                     /locus_tag="P5292_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS38488.1"
                     /translation="MRTLAYPILLLLFIPAFCDAQSIFWTENFNNGCPDNCEAATYTG
                     PNGNWTVTSIGTQGSVANEWFISCSENGEAVGACGAGCGNDQTLHIGSVPCTLCLFCP
                     NGDCGASYNAGPAFSGEDPTTDKRAASPAINTLGRTGLSISFQYIENGAGINDDGSVE
                     YSSDGGATWTQLVNLAKTNTCPSGQGVWTAYNATLPPACENVPDLRIGFRWKNNSDGV
                     GTDPSFAVDNLTLSESSASLPVALFSVSDTLGCDSLCVTFSDLSTGTPTTWNWSFPGG
                     LPATSTAQDPGTICYSTPGTYSVTLTVSNGAGSNTQVTTNLIRIQAGPRADFSCSDST
                     ICAGDSIYFFDQSIGTIASRTWLFAGGTPFTSGDLNPSDILFSNPGAYTITLTVDDGQ
                     CSNSKSQINFITVHGLPQPVLTAAGPDVFCDPIYLSYQWYELSSGQLTETGPSLLGVP
                     SGSYFVFVTDSNGCSGYSDTLAVINDGFGSVHTAPVSVFPNPVGDELILRHIATACYR
                     VELWNLQGQLLQSLGLSCNSGKLPLPSLTPGCYLLRFTNEQGSFQQRLIKR"
     gene            3382..>3708
                     /locus_tag="P5292_04845"
     CDS             3382..>3708
                     /locus_tag="P5292_04845"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HRS38489.1"
                     /translation="MAVRHVLMIAPCGFCSNPETAGDNAFQQQGIIDPLAHQSAALAE
                     FNAAVVRLESAGIRVSRFQPPDEQTPDALFPNNWFSTHEDGTLVLYPMKAVNRRLERR
                     SEFIAWL"
BASE COUNT          862 a         1064 c          957 g          825 t
ORIGIN      
        1 gctcccagcg gtgcattcag cgttacggag ccggtaccgc aatcatacag cgtatccgat
       61 ccggcattga tcgttgccgc gggttgacag gctgtcgagc gcagtcggta aacagtaccg
      121 ccgagcgcag cgacataaag ctcaccccat ttgtcttcgc caaaacctac aatggcgctg
      181 gcacccaaac tacccaggtt ggtatgggtg aagccaccgg ctccgtcagg ttccagcgtc
      241 cggatggaag aaacaccata gtcggagaaa aaatacttgc cataaatatc agcatatcgg
      301 gcgccgcgat acacgtaacc tccagtcacg gaatatccgg acgaacgcgg gtaaaagtga
      361 accggcgaaa cgtaattcga ctgagcgaga caaccagcgg tattgtagct ggcattggct
      421 tcgtaacacc tccagccgta attcaatccg ccgccggaac cgtagggttc gtaatcgatt
      481 tcttcgtagg tcccttgccc tacatctccg atccaaagat ctccggttgc acgatcgaag
      541 ctccagcgcc atgggttccg gacgcccagc gcccagatct cttccgcgcc aagagaagga
      601 tttccggcaa agggattggt tggaggaata ctataggtcg ggttggaagg gtccacggcg
      661 atgcgcaaca tttttccgag caatgaatcc gggttctgcg cgcggttctc cgggtcaccg
      721 ccggaacctc cgtcacccat cccgatatag agatacccgt ccttaccgaa ctggatatgt
      781 ccgccgttgt gattggaata cggctggtgg atcgtcagca ggatttcttc gctattcgca
      841 tccgcgctat cgggattggt tgcactgaca tgaaagcggg agatgcgcgt atcaccgtcc
      901 ggacgacggg tgtagttcac ataaaacacc tgggacgtcg cgaagtcggg aggaaacgcc
      961 agacccagac aaccctgttc gccactgtac aatacccgac cgacaatgtc aagaaacggt
     1021 accgggttga tattgccggc cgtatccaga atgttgatct gaccgttctg ctcgagaacg
     1081 aagatccggc catcgccgca attcttgata tcaaccggtg atgtgaagcc cgaagcgaac
     1141 ggcgtaagcg ttacctggga catggaagga acggtcagcc cgatcaggct gaccaagacg
     1201 cagagtactt ttttcataga aggatgggaa gactagtgct tcttccaaaa gtagcaaaag
     1261 ggcacgtttc ttccccgacc tgaataaaaa aaggggcccg cgggcccctt aacaggtcgt
     1321 tcttccgtaa atcagaaaat taccttaaaa gtgaggaata caatggctga aacaaggatc
     1381 gcgtctacga ccaacagcat atacaagctg atcgggagac tttcctttgc ctgcgcctcg
     1441 ggataaaccg tgcgccctgc atcgtcttcc tccatgtggt tgtaggtaac aacaggttcg
     1501 tttttcatga ggggtggttt taacgcagga tacaaccgga aaagtcgccc gtgtgaacaa
     1561 gcattccgtt ccgctttcat ttgtaagaaa tggtcgaaaa tgcgacatga actccacctg
     1621 agcacgcaaa tcccggcgaa atctcgtttt gcctcttcct gtaccaatgc ttaagtttgg
     1681 gagaaagcca gactccgatg cgaacactcg cctatcccat actattgctg ctcttcatcc
     1741 cagctttttg tgacgcccaa agcatcttct ggacggaaaa tttcaataat ggttgtccgg
     1801 ataattgcga agccgctacc tacaccggac cgaacggcaa ttggaccgta acgtcgatcg
     1861 gtacccaggg ctctgtggcg aacgaatggt tcatcagttg ctccgaaaac ggcgaagcgg
     1921 taggcgcctg cggagctggc tgtgggaacg atcagaccct gcacattgga agcgttcctt
     1981 gtaccctctg cctgttttgt ccgaatggcg attgcggcgc atcctacaat gcaggcccgg
     2041 cattttcagg tgaagacccg accacggata aacgcgcagc atcacccgct atcaacacac
     2101 tcggtcgtac cggactaagc atttcttttc agtacatcga aaacggcgca ggcatcaacg
     2161 acgacggaag cgtggaatac agcagcgacg gtggtgctac ctggacccag ctcgtcaatc
     2221 tcgcaaagac caacacctgt ccgagcggac aaggcgtctg gacagcctat aacgcaacgc
     2281 ttccacctgc ctgtgagaat gtcccggacc ttcggatcgg attccgttgg aagaacaaca
     2341 gcgacggtgt agggaccgat ccttcctttg cagtggacaa cctcaccttg tccgaatcgt
     2401 cggcttccct tcccgttgcg ctcttttctg tatccgatac gcttggctgc gattcacttt
     2461 gcgtaacgtt cagcgatctg agcactggaa ctcctaccac ctggaattgg tcgttcccag
     2521 gcggccttcc cgctacctcc acagcacagg acccgggcac gatctgctat tccactccgg
     2581 gaacttactc cgttacgctg acggtctcca atggcgcagg cagtaacacg caagtgacaa
     2641 ccaatttgat ccgcatccag gcaggtccgc gagcggattt cagttgctcg gattccacga
     2701 tctgcgcagg cgattcgatc tacttcttcg atcaatcgat tggcacgatc gcatcccgca
     2761 cctggctttt tgccggtggc acacctttca cgtccggcga ccttaatcct tccgacattc
     2821 tgttttccaa tccgggtgct tacacgatca ccctgactgt ggacgacgga caatgcagca
     2881 acagcaagtc gcagatcaac ttcattaccg tccacggcct acctcagccg gtgctgaccg
     2941 ctgccggccc ggatgtcttc tgtgatccga tttatctgtc atatcaatgg tatgagctca
     3001 gctcgggtca gttgacggaa accggccctt cgctgctggg agtcccaagc ggttcctact
     3061 tcgtgttcgt taccgacagc aacggttgta gtggatattc cgataccctt gcggtgatca
     3121 acgatggctt tggatccgtt cataccgcgc cggtttcggt ttttcccaat cctgttggtg
     3181 atgagctcat actccggcat attgcaacag catgttaccg ggtcgaactc tggaatctgc
     3241 aggggcaact gctgcaaagc ctggggttaa gctgcaattc gggtaaacta cccctgccgt
     3301 cacttactcc cggatgttac ttgcttcgct ttacgaacga acaaggctct ttccaacaac
     3361 gcctgatcaa acgatgagcg gatggcagtg cggcacgtgc tgatgatagc cccatgcggt
     3421 ttctgctcca accccgagac cgccggcgat aacgcatttc aacaacaggg aatcatcgat
     3481 ccattggcgc atcaatcagc ggccttagct gaattcaacg cggctgtagt ccggctcgaa
     3541 tccgcgggga tccgtgtcag ccgctttcaa ccgcccgacg aacagacgcc cgatgcgctc
     3601 tttccgaaca attggttcag cacgcacgag gatggaaccc tggtgctgta tccgatgaag
     3661 gccgttaatc gcaggctgga acgacgatcg gaattcatcg cctggctg
//
LOCUS       DAOTON010000187         3678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_19983_length_3678_cov_3.897047, whole genome shotgun sequence.
ACCESSION   DAOTON010000187 DAOTON010000000
VERSION     DAOTON010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19983_length_3678_cov_3.897047"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="P5292_04850"
     CDS             complement(<1..935)
                     /locus_tag="P5292_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRS38490.1"
                     /translation="MVLLIFLLFDRLFPLRVSVPYSQVITSSDGSILQAFLSPDDKWR
                     LYVEPDRITPELETAILFKEDKYFHWHPGVNPVAVVRAAWNNLVTGHRTSGASTITMQ
                     VARLLEPAPRTLGSKLRETFRAFQLEWHFSKAEIFRLYLNLVPYGGNVEGIASASYRY
                     FGRTTDQLSLAQIVTLAIVPNRPGSWRPGETNQRLLAGRNRWLGKMRTQELFSEAVIR
                     DALEEPLTIDRPEPARWAPHLTARIHRAIPDQPVVRTTIALRRQQQVQTIVANYQRSL
                     RPYGIHNAAVLVVNNATRAVEAYVGSPDFDDNGHGG"
     gene            complement(1012..>3678)
                     /locus_tag="P5292_04855"
     CDS             complement(1012..>3678)
                     /locus_tag="P5292_04855"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRS38491.1"
                     /translation="NTSFEVNTEGQVDMQADKDEYQVGDKAKILFKTPFNGKLLVTIE
                     TDGLLEHRYLTTDKKGAVLEIPIKQDLLPNFYVSATLFRPLDDGTIPLTVAHGYLPVK
                     VSQASTRLPVSIVAAATSRSKLKQTITVRSAPKQDIEVTVAVVDEGILSVSGYETPDP
                     HGFFYQKKALGVNAYDVYPFLLPDLALRRSSVGGDAEYSKRMGKRNNPLANKRVKLVA
                     AWSGILKTDGRGEAKYTLDIPQFSGDLRVMACAYKDNAFGSAEHHMKIADPVVISTGL
                     PRFLSPRDTVQMPVTLTNTTKQAQRVTVNLSKSGPLAFAGSTSKEVSLPANGEGRILV
                     PLYASASVGAGSVTVAVSMQGETFTDVTDITVRPPATFERRSGSGEVKTRAQFSLSND
                     FVPGSAEATLLVSRNPMVQFTKPLNYLIGYPYGCVEQTTSQAFPQIYYADLVKNMKFK
                     GARGQNPAFNVQEAIRKLQSMQLYNGGLAYWPGGDNESWWGTVYAMHFLCEARKAGYD
                     VNGEVIRKGLAYIAMKTKSHTIEKDYAYDNGSGQTLVRNIYAKENFYGLFVLAAFGKA
                     DLASMNYFKSNISMLALDSRYLLAASYLAVGDRTNYQSLLPTAFAGEASKRVLSGSFY
                     SYLRDQALAASVLLDVDPDNPQIPIMLRHLSQQVNSEPWLNTQEAAFAFMALGKYMRR
                     LPAVSPTATIRVNGKEAGNFAGDDLSLSKGLTGGTVDIEVKGQSPVFYFWQLDGLPAS
                     ARNKESDQFLQVRRRVLNRAGQPVNLNAIRQGELLVIDLSLTSIERSSVENVVVTDLL
                     PAGFEIENPRLTEAREMPWIKDASTPEYFDVRDDRIHFFTTATNTTKHFYYVVRAVSP
                     GTYIQGSVGADAMYDGSYHSYSGSGSVRILAP"
BASE COUNT          793 a         1036 c         1045 g          804 t
ORIGIN      
        1 ccgccatgtc cgttatcgtc gaagtcggga gagccgacat acgcttccac ggcccgggtt
       61 gcgttattga cgaccaggac cgccgcgttg tggatgccgt agggtcggag actacgctga
      121 tagttcgcaa cgatcgtctg tacctgctgc tgtcgacgca aagcgatcgt ggtcctgacg
      181 acaggctgat ccggtatggc acggtggatc cgtgcggtga gatggggtgc ccaacgggct
      241 ggctccggac gatcgatggt caacggctct tccaatgcat cgcggatcac cgcctccgaa
      301 aacaattcct gcgttcgcat tttccctaac caccggttcc gtcctgcgag cagccgctgg
      361 ttcgtttcac cgggacgcca gctgccgggc cggttcggaa cgatggcgag tgtcacgatc
      421 tgcgcaagcg ataactgatc ggtcgtccgg ccgaaatagc gataagaagc cgatgcgatc
      481 ccctctacat ttccgccata aggaacgagg ttcagataaa ggcggaagat ctccgccttc
      541 gaaaaatgcc attcgagctg aaaagcccga aacgtttcac gcaacttact tccgagcgta
      601 cgcggtgccg gttccagcaa gcgggcaacc tgcatggtaa tggtggacgc gccggatgtg
      661 cggtgtccgg tcaccagatt gttccaggcg gcgcgcacca ccgccaccgg attcacgccg
      721 ggatgccagt gaaagtactt atcttctttg aagaggatcg ccgtttcaag ctccggcgtt
      781 atacggtccg gttccacata gagccgccac ttgtcgtccg gggaaagaaa ggcttgtaag
      841 atgcttccgt cggatgaagt aataacttgt gaatagggaa cggatacgcg taagggaaac
      901 agccggtcaa acagcaggaa gatcagcagt accaaaacgg gaaaagcgat gacttttagc
      961 catcgcctcc accgttgcgg tattgatcca atccgcggaa tcattcaagg atcagggcgc
     1021 caggatacgg accgagccgc tgccggaata actgtgataa ctgccgtcgt acatcgcgtc
     1081 ggctccaacc gaaccttgta tataggttcc gggcgacacg gcgcgcacta cgtaatagaa
     1141 atgcttcgtc gtgttggtgg ctgtcgtgaa gaagtggata cggtcatccc gtacgtcgaa
     1201 gtactccggc gtggaggcat ccttgatcca gggcatctcg cgggcttcgg tcaggcgggg
     1261 attctcaatc tcgaaaccgg cgggcagcag atcggttacc accacatttt ccaccgagct
     1321 gcgttcgatg gaggtgaggc tcaggtcgat gaccaacagt tcgccctggc gaatcgcgtt
     1381 caggttgacc ggttgtccgg cacgattgag gacgcggcga cgcacctgca ggaactggtc
     1441 ggattcctta ttccgggcgg aagccggcaa accatcgagc tgccagaagt agaagaccgg
     1501 gctctgtccc ttgacttcaa tatcgacggt gccgcctgta agtcctttcg acaggctcag
     1561 gtcatcaccc gcgaaattac cggcttcttt gccgttcaca cggatggtgg cggtgggaga
     1621 caccgccggc aaacgacgca tgtacttgcc caacgccata aaagcgaagg ccgcttcctg
     1681 tgtgttgagc catggctcgc tgttgacctg ctgactgaga tgtcgcagca tgatcgggat
     1741 ttgcggatta tccgggtcta catccagcaa gacactggcc gcaagcgcct gatcgcgcag
     1801 ataggaatag aaactgccgg agagtacgcg cttcgatgct tcaccagcaa aggcggtcgg
     1861 aagtaacgac tggtaattag tacggtcgcc gactgccaaa taagaagcag ccagcaggta
     1921 cctggaatcg agcgccagca tgctgatgtt gctcttgaag tagttcatcg aagccaggtc
     1981 agctttcccg aaggccgcca gcacgaagag gccgtagaaa ttctccttgg cataaatatt
     2041 ccggacaagg gtttgcccgc tgccgttgtc gtaggcatag tccttctcga tcgtatgcga
     2101 cttggtcttc atggcgatat aggcaagtcc tttacggatc acctcgccgt tcacatcgta
     2161 tcccgctttt cgggcctcac acaagaagtg catggcatac accgtacccc accaactttc
     2221 attatcccct cccggccaat acgccagtcc gccattgtat aactgcatcg attgcaattt
     2281 ccggatggct tcctgaacgt tgaaagcagg attctgaccc cgggcacctt tgaacttcat
     2341 gttcttgacc aggtccgcat agtagatctg cgggaaagcc tgtgaagtgg tttgttcgac
     2401 gcaaccgtac gggtaaccga taagataatt cagcggtttg gtgaactgca ccatcggatt
     2461 tctgctaacc aaaagtgtag cctcggcaga acccggcacg aaatcgttgg acagcgaaaa
     2521 ctgcgcccgt gttttcacct cgcccgaacc gcttcgtcgc tcgaacgtag ccggcggacg
     2581 aaccgtgata tccgttacgt cggtgaaggt ttctccctgc atgctgaccg caacggtgac
     2641 acttcccgct cccaccgaag cgctggcata gagcggaacc aggatacgac cctccccatt
     2701 ggccgggagg ctcacttctt tcgatgtgct tccggcaaac gcaagcggac cgctcttact
     2761 cagattcacg gtgacacgct gcgcctgttt ggtggtgttg gtcagcgtaa ccggcatctg
     2821 tacggtatca cgcggtgaga ggaaacgcgg caagccggtg ctgatgacaa ccggatcggc
     2881 tattttcata tgatgctccg cgctgccaaa cgcgttatcc ttatacgcgc aagccatgac
     2941 gcgcaaatcg ccggagaact gggggatgtc cagggtgtac ttggcctcgc ccctaccatc
     3001 ggtcttcagg ataccgctcc aggctgctac cagtttcaca cgcttattgg ccagggggtt
     3061 gttccgcttg cccatgcgtt tcgaatattc cgcatcacca ccgacactgg aacggcgcaa
     3121 ggcaaggtcc ggcagcagga acgggtagac atcatacgca ttaacgccca gggccttctt
     3181 ctgatagaaa aacccgtgcg gatcgggggt ttcgtatccg ctcacggaga gaatgccttc
     3241 atccacgacg gcgacggtga cttcgatatc ctgcttcggc gcgctgcgta cggtaatggt
     3301 ttgtttcagc ttgcttcgtg aggtggcagc ggcgacgata ctgacgggca ggcgcgtcga
     3361 tgcctggctg acctttaccg gaaggtaccc gtgcgcaacg gtcagcggga tcgtgccatc
     3421 gtccagcgga cggaacaaag ttgcgctgac atagaagttg ggcaacaggt cctgtttgat
     3481 cgggatctcg agcaccgcgc cttttttgtc ggtggtcaga taccgatgct ccagcaatcc
     3541 atccgtttcg atcgtgacga ggagcttacc gttaaacggt gtcttgaaca ggatcttcgc
     3601 cttatcgccg acctggtatt catccttgtc ggcttgcata tcgacttgtc cttcggtatt
     3661 tacttcgaac gacgtgtt
//
LOCUS       DAOTON010000188         3675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20004_length_3675_cov_3.262155, whole genome shotgun sequence.
ACCESSION   DAOTON010000188 DAOTON010000000
VERSION     DAOTON010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20004_length_3675_cov_3.262155"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="P5292_04860"
     CDS             complement(<1..328)
                     /locus_tag="P5292_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRS38492.1"
                     /translation="MLHALGLEQTVVGITRFCTRPESWRSEKVNVGGTKTPDLDKIRA
                     LKPDLILGNKEENSADTIHALENSFPVWMSDISTLGDALDMITRVGNMTGKDLEATSM
                     ASEIQDK"
     gene            complement(439..1824)
                     /locus_tag="P5292_04865"
     CDS             complement(439..1824)
                     /locus_tag="P5292_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRS38493.1"
                     /translation="MASNPSKRVCNNILEAIGHTPLVRLNSVTKGVPATVYAKVETFN
                     PGNSIKDRMALKMVEDAEKAGILKPGGTIIEGTSGNTGMGLAIAAIVKGYKCIFTTTD
                     KQSKEKVDALKAYGADVIVCPTNVEPEDPRSYYSVAKRLNKEIPNSFYPNQYDNLSNS
                     QAHYEQTGPEIWEQTEGKITHLLVGAGTGGTITGTGRYLKEKNPNVKIWGIDTYGSAL
                     KKYHETGVLDKNEIYPYVTEGIGEDFIPLNYDFKIIDHFEKVTDKDAALMTRELCRQE
                     GIWVGNSAGSAIAGLLQLKDQLKPTDVVVVIFHDHGSRYLAKMFNDDWMRKMGYLDTS
                     GMTAQDLVASRKSIEVVTIDKHQPISAALKLITENNYSQIPVTSEDRIVGTIYENTVF
                     NSILKNPAGKSATVESIMKEAMPFVDITTTVHDLSTMVSGEREAVLVKDFKAGRNYII
                     TKSDIAEALAR"
     gene            complement(1911..3101)
                     /locus_tag="P5292_04870"
     CDS             complement(1911..3101)
                     /locus_tag="P5292_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS38494.1"
                     /translation="MRSGMDTLRVSRPVVRLAISGIALGFSAMIISVAIVKGFKQEIR
                     EKVIGFSAHVQISKFDANQSFESSPIEFNTANLSSIAKLEGVRHVQAFATKAGIVRKG
                     SEIEGVIAKGVDSGYDWSFFRKSLRAGAIPVYANSSRSSDILISTVLARKLNLNVSDS
                     LVTYFVQQPPRARKFKIVGIYETGLEEFDEKFVFCDLAQIRKLNDWNDSTIGGLEVQL
                     SDFDALDEAAPRIYATTGSDLDVRTIREMYPQIFDWLGLQDINAVVIIILMIIVAGIN
                     MIAALLVLILERVGTIGTLKAIGASDASVRNIFLRIAALLISRGLFWGNVVGIGICLL
                     QHYFHWIRLDQDSYYIAYVPIRLLLLDLLWMNLGTLLVCTMMMLVPTLLVTRIRPVRA
                     LRFR"
     gene            3191..>3675
                     /locus_tag="P5292_04875"
     CDS             3191..>3675
                     /locus_tag="P5292_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRS38495.1"
                     /translation="MLFRCLWLFILLTQVDATAGSPGRIVTDQDVTVGAARFDQYLDL
                     LKRKKIAIVANPTSRVGNVHLVDTFLSLGVHIGCIFAPEHGYRGEAAAGEHVKGGKDR
                     ASGIPVISLYGKHNKPKQDDLKGIDLVVFDIQDVGARFYTYSSTLQNVMEACADFNKP
                     LL"
BASE COUNT          848 a          918 c          929 g          980 t
ORIGIN      
        1 atttatcctg aatctctgaa gccatactcg tggcttcgag atcctttccg gtcatgtttc
       61 cgacacgcgt gatcatgtcc agcgcgtctc ccaaagttga aatatcactc atccagacag
      121 gaaaggagtt ttccagggca tgaatcgtat ccgctgaatt ttcctcctta tttcccaaga
      181 tcaaatcggg ctttagtgcg cgaatcttgt cgaggtctgg tgtcttggtt ccgccgacat
      241 ttactttttc actgcgccag gattccggac gtgtgcaaaa acgggtgatt ccaacaacag
      301 tttgttccag acccagcgca tgaagcaact ccgtttggga cggtaccagg gatacgattc
      361 tacgcggata gtcccgcaat cggatcgttc gacccatttg gtctgtgatg acctgcatgt
      421 acctcctgtt atccctgttt atcgggcaag ggcttccgca atgtcgctct tggtgatgat
      481 gtagtttcga cctgccttga aatccttaac aagtacagcc tcgcgttcac cgctaaccat
      541 cgtggaaaga tcatgaacgg tggtcgtgat gtccacaaac ggcattgctt ctttcatgat
      601 ggattctacg gttgccgatt tacccgccgg attcttgaga atgctgttga aaacggtgtt
      661 ctcgtaaatg gtgccgacga tccggtcttc cgatgtgacc gggatctgtg aatagttgtt
      721 ttccgtgatc aacttcaatg ccgcgctgat cggctggtgt ttgtcaatgg tcaccacttc
      781 gatgctctta cgggatgcca ccagatcctg agcggtcatg ccgctggtat cgaggtagcc
      841 cattttacgc atccagtcgt cgttgaacat cttcgccaga taccgtgaac catggtcgtg
      901 gaaaatgact accacgacat cggtgggttt caattggtct ttcagttgaa gcaatccggc
      961 tatggctgaa ccggcggagt tgccgaccca gatgccttcc tgacggcaaa gttcacgtgt
     1021 catgagggca gcatccttat ccgtgacttt ttcgaagtga tcgatgatct tgaaatcgta
     1081 gttgagcgga atgaaatcct cgccaatacc ttctgttaca taagggtaga tctcgttttt
     1141 atcgagtact ccggtttcat ggtatttctt caacgcggag ccatacgtgt cgatccccca
     1201 gatcttgacg ttcggatttt tctctttcag gtatcggccc gtaccggtga ttgttccgcc
     1261 ggtaccggcg cctaccagta aatgtgtgat tttaccttcg gtctgttccc agatttccgg
     1321 gccggtttgc tcgtaatggg cctgtgaatt ggaaaggttg tcgtattgat tcggataaaa
     1381 ggaattcggg atctccttgt taagtcgttt ggcaactgaa tagtaggagc gagggtcttc
     1441 cggctctaca ttcgtcggac acacgatcac atcggccccg taagccttaa gggcgtcgac
     1501 tttttccttt gactgcttgt cggttgtggt gaatatgcac ttatagcctt ttacaatagc
     1561 cgcgatggca agtcccatcc cggtatttcc ggatgtgccc tcgatgatcg ttccgcctgg
     1621 cttcaggatg cccgcctttt cggcatcctc taccatcttg agggccatgc ggtccttgat
     1681 ggaattaccg gggttgaaag tctcgacttt agcatagact gttgccggaa cgcctttggt
     1741 aacgctgttg agccgtacaa gcggcgtgtg tccgatcgct tcgaggatat tgttgcaaac
     1801 tcgcttagag ggattgctgg ccataataag tggtttttac gaaaaaggcc atttttggca
     1861 cattttcgag ggctaaaggt agtaaaattg gcgcgaaagg aactctttca tcatcgaaac
     1921 cggagggcgc gaacgggccg gatccgggtc accaaaaggg tcgggaccag catcatcatg
     1981 gtgcaaacca agagggtgcc aaggttcatc caaagcaggt caagaagaag cagtcggatc
     2041 ggaacatagg caatgtaata ggagtcctga tccagtcgaa tccagtggaa atagtgttga
     2101 agtaaacata ttccgatgcc gactacgtta ccccagaaca aaccgcgact gatgagtagg
     2161 gcggcgattc ttagaaagat gttgcgcaca gaagcatcgc ttgcgccaat cgctttcagt
     2221 gttccgatcg ttcccacccg ttccagtatc aataccagga gcgcagcgat catgttaatg
     2281 cccgctacaa tgatcatcag gatgatgatc accaccgcat tgatgtcttg cagtcccagc
     2341 cagtcaaata tctgcggata catttcccgg atcgttcgta catccagatc agagccggtt
     2401 gtcgcgtaaa tgcgcggagc cgcttcatcc aatgcatcga aatccgagag ttgtacctcc
     2461 aagcctccga tggtagaatc gttccaatcg ttcagttttc gaatttgtgc caggtcgcag
     2521 aagacgaact tttcatcgaa ttcctccaag ccggtttcgt agatgccgac gatcttgaat
     2581 ttgcgtgccc ggggcggttg ctgaacgaag taggtgacca ggctgtcgct gacgttcagg
     2641 tttaattttc ttgcaagaac agtcgaaata agaatgtcgg agcttctgga ggaatttgca
     2701 tacactggaa ttgctcctgc gcgcaaggat ttcctgaaaa atgaccagtc atacccggaa
     2761 tctacgccct tggctataac gccttctatc tcggagcctt tccgtacgat tccggctttc
     2821 gtcgcaaagg cttgcacgtg tcgcacacct tccagtttcg cgattgacga taaattcgct
     2881 gtgttgaatt caatcggact tgattcaaag gattgattgg catcgaactt gctaatctgc
     2941 acatgagcac tgaatccaat gaccttttcc cggatctctt gcttgaatcc tttcacgatc
     3001 gcgaccgaaa taatcatcgc ggaaaatcca agcgcgatac cggaaatggc caatcgcacc
     3061 accggtctgg atacacgcag ggtgtccatt cccgatcgca tgaggcgccg tgcaatccat
     3121 tgttcgtaac ccacagaact gaaatccttt ggaaagagaa atcgttcgaa agatacctac
     3181 ttttaggcgt atgcttttcc gttgcttatg gttatttatt ctgctgacgc aggtcgatgc
     3241 cacagctggc agtcccggac ggatagtaac cgatcaggat gtcactgtcg gagccgcgcg
     3301 ctttgaccag tacctggacc tgttaaaaag aaagaagatc gccatagtgg cgaacccgac
     3361 ttcgcgtgtc ggaaacgttc acctggttga cacgtttctt tcattgggtg ttcacatcgg
     3421 ttgcatattc gcacccgaac atggttaccg gggcgaggcg gccgctggag aacatgtcaa
     3481 aggagggaaa gatcgtgctt ccggcattcc cgtcatttcc ctctacggga agcacaataa
     3541 accaaagcag gatgatctga aggggatcga ccttgtggta ttcgatatcc aggatgtcgg
     3601 ggccagattc tatacgtaca gctccacact tcagaatgtg atggaagcct gcgcggattt
     3661 caataagccg cttct
//
LOCUS       DAOTON010000189         3674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20010_length_3674_cov_3.509257, whole genome shotgun sequence.
ACCESSION   DAOTON010000189 DAOTON010000000
VERSION     DAOTON010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20010_length_3674_cov_3.509257"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="P5292_04880"
     CDS             complement(<1..682)
                     /locus_tag="P5292_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38496.1"
                     /translation="MTKKMYNVHKTTGIRATARALFFLVALLLGGRTANASHMAGADL
                     TYEYLGNGQYLVTYTLYRDCFGIPAPTSAFMTMSSASCNNFSQSVTLNAVPGTGQEIS
                     YACPGTLTTCSGGTAPGFQEWEYSAIVTLPAQCPDWTFSVSDCCRNGAITTIGTPDSY
                     SLYIEAKLNNTVFDNTSPTFTNVPVTFLCIGQDNYYNHGVIDAEGDSLAYFFIPPRDA
                     QNTNLAYQP"
     gene            complement(894..1190)
                     /locus_tag="P5292_04885"
     CDS             complement(894..1190)
                     /locus_tag="P5292_04885"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRS38497.1"
                     /translation="MINRLVKLSFHAEDAGKFTELFREVSGKIRAFPGCLHLELWEDL
                     REKGVFFTFSQWDSEASLDHYRFSPLFKSVWAHVKPLFKAPAEAWSVRKNPVDS"
     gene            complement(1183..1977)
                     /locus_tag="P5292_04890"
     CDS             complement(1183..1977)
                     /locus_tag="P5292_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRS38498.1"
                     /translation="MAFLTLITDWGLRDHYVASFKGKLLSRSADLGIIDISHEIDPFN
                     TLHAAFVLRNAYSAFPTGTVHYIGLDGAGNSAQDEPGRDYLIVTANGHHFVGVDSGIF
                     SLLLGEEEKKIHRLPIDPIRTGHREVQDQLVDVLIKLCTGKHPRQLGLEVDHLLPSFF
                     AQPTVDPGGIRGALVYVDGFGNSIFNISRELFERERKDRSYSILLRRANYTVRQIMHD
                     YGSVETGEIVALFNQDGYLEIAINRDNASKLLGLKQFDTLRIEFHD"
     gene            2143..3087
                     /locus_tag="P5292_04895"
     CDS             2143..3087
                     /locus_tag="P5292_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRS38499.1"
                     /translation="MSEVIIPIDTFNPVEFFGVNDANLDIIRKNFPELKLVARGNEIK
                     VIGDEKNIHLFSEKINKLIQHYQKVGSLTPDNVLHFLGDRDGAAPPPSPQTADVLVFG
                     QNGLMVRARTANQKRMVESSEKNDVVFAIGPAGTGKTYTAVALAVRALKNREIKRIIL
                     TRPAVEAGENLGFLPGDLKEKIDPYLRPLYDALYDMIPSDKVKSYIENGIIEVAPLAF
                     MRGRTLDNAFVILDEAQNATESQLKMFLTRMGPSAKFIVTGDVTQIDLPKHQPSGLVQ
                     AIKILSNVRGIDFIYLDAQDVVRHRLVKEIIEAYNKDR"
     gene            3091..>3674
                     /locus_tag="P5292_04900"
     CDS             3091..>3674
                     /locus_tag="P5292_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRS38500.1"
                     /translation="MMHAMQATAVNTTDFNFPGQSAFYRGKVRDVYTIDGKYLVMIVT
                     DRISAFDVVLPRPIPHKGAVLNQIAAKMLQATADIVPNWVIDVPDPNVTIGRVCSTFK
                     VEMVIRGYLSGHAARTYASGGRVLCGVALPEGMKENDRFPTPIITPTTKAAAGHDEDI
                     SREDIIAKGIVSEEDYTRIESYTQQLFKRGSEIAA"
BASE COUNT          905 a          954 c          996 g          819 t
ORIGIN      
        1 ccggctggta agccaggttg gtgttctgag cgtcgcgcgg cgggatgaag aagtaagcga
       61 gcgaatcgcc ttctgcatca atgacaccgt ggttgtaata gttatcctgg ccgatgcaca
      121 ggaaggtaac cggtacgttg gtaaaggtcg gcgaagtgtt gtcgaatacg gtgttgttca
      181 acttcgcttc gatgtacagg ctgtagctgt cgggcgtacc gatcgtcgtg atcgcgccgt
      241 tgcggcaaca atcggaaacc gagaacgtcc agtcaggaca ctgagccgga agcgtaacga
      301 ttgccgagta ttcccactcc tggaaaccag gtgctgtacc acccgaacag gtcgtcagtg
      361 tgccgggaca agcataagag atttcctgtc cggttccggg taccgcgttg agtgtaacgg
      421 actgcgagaa gttgttgcag gacgctgaac tcatcgtcat gaaagccgat gtcggtgcag
      481 gaataccgaa acagtcacgg tacaatgtgt aagtcacaag gtactgtccg ttgcccaggt
      541 attcatacgt caggtcggca ccagccatgt gactggcgtt ggccgtccgt ccacccagca
      601 gcagtgctac caggaagaag agggctcgag ctgtcgcgcg aatccccgtt gttttgtgta
      661 cgttgtacat ctttttagtc atattgattg gagttgattc gtagtgtacg cagttaagat
      721 agggctaaat gtactatggg agcgatcggt aagcctaaat tcttcggtga aaggcggaag
      781 cggagtggtg aaaggcatga cttccgttgc gaatcttggc cggattttga tcgttagatg
      841 aagcaatatt attcataaaa cccgtatctg acaaggcttt cacgggtaat ctttcaagaa
      901 tccaccggat tcttgcgaac actccaggct tccgcaggag ccttaaaaag gggcttaaca
      961 tgcgcccaaa cggacttgaa aaggggggaa aaccggtaat ggtccaggga agcttccgaa
     1021 tcccattgag aaaacgtgaa aaaaacgcct ttttcacgta aatcttccca taattcaagg
     1081 tgcagacagc ccgggaatgc ccggatcttc ccggaaactt cccgaaatag ctccgtgaac
     1141 ttccctgcat cctccgcgtg aaaactaagc ttgaccaacc ggttaatcat gaaactcgat
     1201 gcgtagggtg tcaaattgtt ttaagccaag cagtttggat gcattgtcac gattgatggc
     1261 gatctctaaa tacccgtcct gattgaacag ggcgacgatc tcaccggttt caaccgatcc
     1321 gtaatcatgc atgatctgcc ggaccgtgta attggctcgc ctcagcaata tcgaatagga
     1381 ccggtccttt cgttcgcgtt caaagagttc gcgggaaata ttgaaaatgc tgtttccgaa
     1441 gccatccaca taaacgagtg cgccgcgtat gccacccgga tcaacggtcg gctgagcaaa
     1501 gaaactcggt aaaagatggt cgacctccag gccgagttga cgcggatgct tgccggtaca
     1561 gagttttatc aggacatcga ccagttggtc ctgcacttcc cggtgccccg tacgaatcgg
     1621 atcgatcggc aggcgatgaa ttttcttttc ctcctcgccc aacagcaggg agaatattcc
     1681 actgtcgact ccgacaaagt gatgtccgtt cgccgtcacg atcagatagt cacgccctgg
     1741 ctcgtcttgt gccgaatttc cggcgccgtc cagtccgatg taatggaccg taccggtggg
     1801 aaatgcggaa tacgcgttgc gtaagacgaa tgcagcgtgc agggtattga acggatcgat
     1861 ctcgtgagaa atatcgatga tgccgagatc agcggatcgg gagagcagct tgcctttgaa
     1921 tgaagcgacg taatgatcgc gtaatcccca atcagtaata agcgtaagga aagccatcgt
     1981 tctgctgcgc cccggtgttg aaaacttctg atcctgcttc ggtcaaaagt aatggctttt
     2041 ggggtactat tgaatcgacg ctcgcgcaaa tccattcaca ccaaaggcta tccttggtgt
     2101 tgataacttg taaccggatt tcgcgaccca atccagcccg acgtgagtga agtaatcatt
     2161 cctatcgaca cgttcaaccc ggtggaattt ttcggtgtga acgatgcgaa cctcgacatc
     2221 atccgaaaga attttcccga actcaaactg gttgcccgtg gcaatgagat caaggtcatc
     2281 ggcgatgaga agaacatcca cctcttcagc gaaaagatca acaagctgat ccagcactac
     2341 cagaaggtcg ggagcctgac acccgataac gtgctccatt tcctgggcga ccgggatggc
     2401 gccgcaccac cacccagccc gcagacggcc gatgtcctcg tctttggcca gaacggactc
     2461 atggtccgcg cccgtacggc taaccagaaa cgaatggtgg agtcgtccga aaagaacgac
     2521 gtggtgttcg cgatcggtcc cgccggtacc ggtaagacct atacggctgt cgccctcgca
     2581 gtacgtgcac tgaagaaccg ggagatcaag cgtatcatcc tcacccgtcc ggcagtggaa
     2641 gccggggaga acctgggctt tctgcccggc gacctcaagg aaaagatcga tccgtatctc
     2701 cgtccgctct atgatgccct ctacgatatg atcccttccg ataaggtgaa gtcctacatc
     2761 gagaacggaa tcatcgaagt agcgccgctg gccttcatgc gtggcagaac actcgacaat
     2821 gcctttgtca tcctcgacga ggcccagaac gcaaccgaga gccagttgaa gatgttcctc
     2881 acccgaatgg gtccttcagc caagttcatc gttacgggcg atgtgacgca gatcgatctg
     2941 cccaaacacc aaccgtccgg actggtgcag gccatcaaga tactgtccaa tgtgcgggga
     3001 atcgatttca tctatctcga cgcgcaggat gtcgttcgcc accgtctggt gaaggagatc
     3061 atcgaagcgt ataacaagga ccgctgatcc atgatgcacg ctatgcaggc aacggcagtc
     3121 aacacgacgg actttaattt ccccggccag tccgcttttt accggggcaa ggtgcgggat
     3181 gtctatacga tcgatggtaa atacctggtc atgatcgtta cggatcggat atccgcgttc
     3241 gatgtggtgc ttccgcgtcc cattcctcat aaaggggccg tgctcaatca gatcgcagcg
     3301 aagatgctgc aggcaacggc ggatattgtt ccgaattggg tgatcgatgt cccggatccg
     3361 aatgtgacga tcggacgggt ttgcagtacg tttaaagtgg agatggtcat tcggggctac
     3421 ctctccggcc atgccgcacg cacctatgct tcgggtggcc gcgtactttg tggtgtggca
     3481 ctcccggaag ggatgaagga gaatgaccgt tttcccacac cgatcatcac gcccacaaca
     3541 aaggctgctg caggacacga cgaggatatc agccgggaag acatcatcgc taaaggaatc
     3601 gtttcggaag aagattatac caggatcgaa tcctatacgc aacaattgtt caaacgcgga
     3661 tccgagatcg cagc
//
LOCUS       DAOTON010000190         3660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20106_length_3660_cov_3.408044, whole genome shotgun sequence.
ACCESSION   DAOTON010000190 DAOTON010000000
VERSION     DAOTON010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20106_length_3660_cov_3.408044"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..842)
                     /gene="kdsB"
                     /locus_tag="P5292_04905"
     CDS             complement(108..842)
                     /gene="kdsB"
                     /locus_tag="P5292_04905"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRS38501.1"
                     /translation="MNIIGIIPARYKSTRFPGKPLVNINGKSMIQRVYEQALQSTRLG
                     KVVIATDDDRILKHVQEFNGEVVMTAPHHLSGTDRCAEVIRQDKDKRWDVAINIQGDE
                     PYIQPEQIDLLCSCFKKEETSIATLVKKIVSPDELFNHNNVKVVMNKHGHALYFSRFP
                     IPYNRNFPEQEWLKHSTYYKHIGIYGYRTDTLLEIAGLTKTNLEITESLEQLRWIEHG
                     YTIHAAVTTLESVSIDTPEDLAKVNR"
     gene            complement(1009..1611)
                     /locus_tag="P5292_04910"
     CDS             complement(1009..1611)
                     /locus_tag="P5292_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRS38502.1"
                     /translation="MNRHLATLLLLLVLSMGAKAQYFRLENHYITAGYGFGSFIQAIQ
                     SEVDDVTSTYSTSATGPVYLKYEHALTGKFGIGVAVAYANFGFDYTEKDASGTTTYSH
                     ETDYTTFSILARMNWHFAEKEGFDPYIGVGLGYRDGNWTYKTNDPDGSDEINYSVSFP
                     FGFETTIGARIRLSGGLSAYTEIGLAKSVVQLGLTQRIGR"
     gene            complement(1699..>3660)
                     /locus_tag="P5292_04915"
     CDS             complement(1699..>3660)
                     /locus_tag="P5292_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS38503.1"
                     /translation="EQRIEDVTVSMEVIKPAIIENNNSTSMEDAMQFIPGVNIVDGQA
                     NIRGGSGWSYGAGSRVQVLCDDLPQLAADANDAKWSFLPVENLEQVEVIKGASSVLFG
                     SSALNGVINIRTAYPKDTPITKINYFAAAYDKAKLSLDGITYRLDWWGSAPRTYSGMN
                     FFHSRKQGRFDLVVGGNMFYDEGYRQGEDEQRARINLNARYRFAKIEGLSAGVNFNAM
                     KTEGTLFFLWMNDSTGAYRPAPGTLSDYTTYRTNVDPFITYVDTKGNSHKLRTRWFNT
                     TNENNTNQNSTADLYYAEYQYQKRFSDQVTLTGGLLNSSSKVNSELYGNHTARQQAGY
                     LQADLKWKFITLSLGGRVEQNRINDETDDWVPVYRSGINLHVLQETYLRASYGQGYRY
                     PSIAERFVRTNVGALIVYPNEDLTAERGYSSEIGIKQGLKAGSWAGYLDLAAFQNNYD
                     NMMEFCFAQWGTFTDPLAGNGFRSFNVGNTRIRGAEITLVGAGALYRDLRLNVLAGYT
                     YMDPRQLTYDSLYITKVGLENYRGSDSSDFLKYRFKHLVRFDAELVWKAYSLGANVRY
                     NSFMENIDRIFVTGLFDFAFPPGLGIGHYRKFRNKGDTVVDLRAGWQASKHFRASVVV
                     KNVFNYIYMARPADMQPPRVWVLQVGFQF"
BASE COUNT          757 a          932 c         1072 g          899 t
ORIGIN      
        1 ttcaacgttc aaccttcaac gttcaacctt caacgttcaa ccttcaacgt tcaacgttaa
       61 aacgttacaa cgttacaacg ttacaacgtt acagcgatcc cacaaaatca ccggttcacc
      121 ttcgccagat cctccggcgt atcgatggag acactttcga gtgtggtgac ggccgcgtga
      181 atggtgtaac cgtgttcgat ccagcgcaat tgctccagcg attcggtgat ctcgagattg
      241 gtcttggtca ggccggcgat ctccagtaag gtatcggtgc gatagccgta aattccgatg
      301 tgcttataat acgttgaatg ctttagccac tcctgctcgg ggaaattgcg gttgtaagga
      361 atcggaaagc ggctgaagta cagtgcgtgg ccgtgcttgt tcatcaccac ttttacattg
      421 ttgtggttga acagttcgtc gggtgatacg atcttcttga ccagtgtggc aatgctggtc
      481 tcttcttttt tgaaacagga gcacaacagg tcgatctgtt ccggttgaat gtagggttcg
      541 tcaccctgga tgttgatggc aacgtcccaa cgcttgtctt tgtcctgccg gatcacttcc
      601 gcgcagcggt cggttccgct caggtgatgc ggagcggtca tcacgacttc tccgttgaat
      661 tcctgaacgt gctttaaaat gcgatcgtcg tcggtggcga tcaccacctt gcccaaccgg
      721 gtgctctgca aggcctgttc gtaaacccgc tgaatcatcg atttaccgtt gatattgacc
      781 aggggtttgc ccggaaagcg cgtcgactta taccgggccg gaatgatgcc gataatgttc
      841 atgggtgatt cgtccgtccg gtacaaatat aagcggaagt aggtttgggg ttccgggttt
      901 cgggtttcga gtttccggtt tttattcggt gaggcctatg actacagcct ttattttcaa
      961 atctgtctca taaccggaaa ccggaaactg gaaacccgaa accaatgact atcgtccaat
     1021 ccgctgggtc aaaccaagct gcaccacgga tttggccagg ccgatctcgg tgtaggcgga
     1081 gagaccgccg gacaggcgga tgcgggcgcc aatggtcgtt tcgaagccga acggaaaact
     1141 aacggaatag ttgatctcgt cgctcccgtc ggggtcgttc gtcttgtaag tccagttacc
     1201 gtcgcggtat ccgagtccca ccccgatata cgggtcaaat ccttccttct cggcgaagtg
     1261 ccagttcatg cgggccagga tggaaaaggt cgtatagtcg gtttcgtggc tataagtcgt
     1321 ggtgcctgac gcgtccttct cagtgtagtc gaagccgaag tttgcatagg caaccgcgac
     1381 gccgataccg aattttccgg tgagcgcgtg ttcgtacttg aggtaaacgg gaccggtcgc
     1441 ggaggtcgag taggtgcttg tcacatcgtc cacttcactc tggatcgcct ggatgaatga
     1501 tccgaatccg taaccggcgg tgatgtaatg attctctaat cggaagtatt gagcctttgc
     1561 gcccatggaa agcacaagca gcagcaacag ggtggcgaga tggcgattca tggggtaaag
     1621 ataggggttg gtgacaagtg gacagtgagg cttgtccgtc gatgcttttt cggtggtgga
     1681 atggggagag attggcgatt agaattggaa gcctacttgt agtacccaca ccctcggagg
     1741 ttgcatatcg gccgggcgtg ccatgtagat gtagttgaaa acattcttga caacgacgga
     1801 tgcgcggaag tgtttgctcg cctgccagcc ggcgcgcagg tcgacgacgg tatcgccctt
     1861 gttccggaac ttgcggtaat ggccaatacc caggccgggc gggaaagcga aatcgaagag
     1921 tccggtgacg aagatccggt cgatgttctc catgaaactg ttgtaacgca cgttcgcgcc
     1981 gagtgaatat gctttccaga ccagttcggc atcgaaacgg acgaggtgct tgaagcggta
     2041 cttgaggaaa tcactcgagt cggagccgcg gtagttttcc aatccgacct tggtgatata
     2101 gaggctgtcg tacgtcaatt gtcgcggatc catgtaggtg taacccgcca gcacattgag
     2161 ccgaaggtcg cggtagagtg cgccggctcc aacgagtgtg atctccgcgc cgcgaatgcg
     2221 cgtattgcct acgttgaatg agcgaaatcc attaccagcc agcggatcgg taaacgttcc
     2281 ccactgggcg aagcagaact ccatcatgtt gtcgtagttg ttttgaaaag cagccaggtc
     2341 aagatagccg gcccacgacc cggctttcag accctgctta atgccgatct cgctgctata
     2401 tccgcgttcg gcggtcaaat cttcattcgg ataaacgatc aatgctccga cattcgtgcg
     2461 tacgaaacgt tccgcgatcg atggatagcg atagccttgt ccgtacgacg cgcggaggta
     2521 agtctcctgg aggacgtgca ggttgatacc gctgcggtat accggcaccc agtcgtcggt
     2581 ttcgtcgttg atacggttct gctccacacg tccaccaagc gagagggtga tgaacttcca
     2641 tttcaggtcg gcctggagat agcccgcctg ttgccgggcg gtgtgattgc catacaactc
     2701 ggaattcacc ttcgacgagc tgttcaagag tccgccggtg agggttacct ggtcactgaa
     2761 gcgcttctgg tactggtact cggcgtagta gaggtcggcc gtggagttct ggttcgtgtt
     2821 gttttcgttg gtggtgttga accagcgcgt gcgcagtttg tgcgagttcc ctttggtgtc
     2881 gacataggtg atgaacggat cgacgttggt acgataggtc gtgtagtcgc tgagcgttcc
     2941 cggtgccggc ctgtaagccc cggtactgtc gttcatccag aggaagaaca gggtgccttc
     3001 ggtcttcatg gcgttgaagt tcacaccggc cgaaaggcct tcgatcttcg cgaagcgata
     3061 gcgggcgttg aggttgatgc gtgcacgctg ctcgtcttcg ccctggcggt atccttcgtc
     3121 gtagaacatg tttccaccga cgacaaggtc gaagcggcct tgtttacggc tgtggaaaaa
     3181 gttcataccc gaatacgtac gcggcgcaga cccccaccag tcgaggcgat aagtgatgcc
     3241 gtcgaggctc agttttgctt tgtcgtacgc ggccgcgaag tagttgattt tggtaatcgg
     3301 cgtgtccttg ggatacgcgg tgcggatgtt gatcacacca ttcagcgcgc tggagccgaa
     3361 gagtacggaa gaggcgcctt tgatcacttc cacttgttcg aggttctcga ccggaagaaa
     3421 gctccacttg gcgtcgttgg catcggcagc cagttggggc agatcgtcgc agagtacctg
     3481 cacacggctc ccggcgccgt agctccagcc actgccgccg cggatgttgg cctgaccgtc
     3541 gacgatatta acgccgggaa tgaactgcat agcatcctcc atcgaggtgc tgttgttgtt
     3601 ctcgatgatc gccggcttga tcacctccat ggagacggtc acgtcttcga tgcgttgttc
//
LOCUS       DAOTON010000191         3660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20108_length_3660_cov_2.598336, whole genome shotgun sequence.
ACCESSION   DAOTON010000191 DAOTON010000000
VERSION     DAOTON010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20108_length_3660_cov_2.598336"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..408)
                     /locus_tag="P5292_04920"
     CDS             complement(1..408)
                     /locus_tag="P5292_04920"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRS38504.1"
                     /translation="MYQHETRFRVRYGETDRMGYVYYGNYATYYEVGRVEALRSLGFS
                     YRSLEDSGVLLPVSDFSVRYRKPASYDDLLVLTTYITALAGVRLHFRYELRNEAGDLL
                     NEAETTLVFVSKASGKPCQPPDELVAALKKFLP"
     gene            576..1325
                     /locus_tag="P5292_04925"
     CDS             576..1325
                     /locus_tag="P5292_04925"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRS38505.1"
                     /translation="MLRSGRLLFSLFLLSGWACSRPALQRVQLQEYVFSDSTYTTQDP
                     KIEGRIAPYRDSLNRSMARVLAESAQPLEKKQPEGKLGDFVADACLQEASALTRERID
                     LALFNHGGLRRSLPMGAITLGDVYELMPFDNELVVITLRGSKLLELLNHVASTGGAPV
                     SGIRMLISGDLADSVRIGGEPLDTNRTYRVLTSDYLANGGDRYTAFADALEKEELRLR
                     VRDAIVQRLEKLGQQRQTLVVTLDGRIQHAE"
     gene            1315..2286
                     /locus_tag="P5292_04930"
     CDS             1315..2286
                     /locus_tag="P5292_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HRS38506.1"
                     /translation="MQSNPSRHTSGLVSRRSFLRYASGTAALIAAGMIPVPAFARTDS
                     IKLTILHTNDVHSRIDPFPDNDPKYPGLGGFARRSALLRQIRSEESHVLLLDAGDIFQ
                     GTPYFNLYKGALELQLMSEMGYDAVTIGNHDFDGGIDNLRERIEQDARFPFLNANYEL
                     QDTPLNGRVLPYKIFRRGGLKIGVLGIGIELQGLVDARMYGNARYLDPVSIAEQTARR
                     LKHDEKCDLVIALSHLGYRYKEKKVSDVILAEETSSIDLIIGGHTHTFLDEPVQLSNK
                     AGKTVHVAQVGWAGIRLGRIDYFFDRDRKKSSLLGGQVKLSDYTIGA"
     gene            2465..>3660
                     /gene="dnaA"
                     /locus_tag="P5292_04935"
     CDS             2465..>3660
                     /gene="dnaA"
                     /locus_tag="P5292_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRS38507.1"
                     /translation="MEKTCESVWKKCLQIIKDNVSPQSFKTWFEPIKPIRLDGKVLTI
                     EVPSQFFYEWLEEHYITLIKKVIRQQLGPEGRLEYSIVMEHAVAEKPYTVRIPTKTSG
                     DLKNSPVSVPVNVSSTIRNPFIIPGLKKVNVESQLNPNYTFENFVEGDCNRLARSAGY
                     AVAGKPGGTAFNPLLIYGGVGMGKTHLAHAIGIEIKNSHPGKTVLYVTSEKFTLQFID
                     AVRNNEQNDFVHFYQMMDVLIIDDIQFLAGKEKTQDVFFHIFNHLHQTGKQIILTSDK
                     APVDLQGMEQRLLSRFKWGLAADLQMPDLETRISILNRKMYQDGIELPKEIVEYIAYS
                     ITSNVRELEGALISILAQASLNKKSVTLELAKQMIDKFVKSTTHEVSIDYIQKIVCDY
                     FDLPIE"
BASE COUNT          908 a          985 c          966 g          801 t
ORIGIN      
        1 tcagggcagg aacttcttta gcgctgcgac cagttcatcc ggaggctggc agggcttccc
       61 ggaagctttg ctcacgaata ccagggtggt ttccgcttca ttcaggagat cgccggcttc
      121 attccggagt tcataccgga agtgcagcct gacaccagcg agcgccgtga tgtaggttgt
      181 caggaccaac aaatcatcat aagacgcagg cttgcggtaa cgaaccgaga agtcggatac
      241 gggaagcaga acaccgctgt cctctaagga acgatagctg aaacccaggc tgcgtaaggc
      301 ttccactcgt ccaacttcgt aataggtggc gtagttgccg tagtacacgt atcccatccg
      361 gtctgtttca ccgtatcgta cgcgaaatcg ggtttcgtgc tggtacattg gaaagaaatt
      421 ccattagcga acgacgaagg tactaacgag tacatttttt cctatacttg tatgacggaa
      481 tcccgcagcg gacgcgccct tcctacactt cgcgtttttc gtccgtcagg ttcccttcag
      541 ttcatccatt cacctccaat caccggcttc acgtcatgtt gcgttccgga aggttattgt
      601 tttcgttatt cctgctgagc ggttgggcct gttcccgtcc tgccttgcag cgcgtgcaat
      661 tgcaggaata tgttttcagt gacagcacct acacaacgca ggatccgaag atcgaaggac
      721 ggatcgctcc gtatcgggac agtttgaaca ggtcgatggc gcgggtcttg gccgaatcgg
      781 cccaaccgtt ggaaaagaag cagccggaag gcaagctcgg cgacttcgtg gccgatgcct
      841 gcctgcagga ggcttccgca ctcacccggg aaaggatcga ccttgcccta ttcaatcacg
      901 gaggattgcg acgttcgctt ccgatgggtg cgataacgct cggagacgtg tacgagctca
      961 tgccgtttga caatgaattg gtcgtcataa cacttcgcgg gagcaagttg ctggaactgt
     1021 taaaccatgt tgcttccacc ggtggcgctc cggtttcggg cattcggatg ttgatcagcg
     1081 gcgacctggc ggactctgtc cggatcggcg gcgaacccct ggacaccaac cgaacctacc
     1141 gggtgttaac gtccgattac ctggcgaacg gcggcgatcg ttataccgcg tttgcggacg
     1201 ctttggaaaa agaagaactg aggcttcggg tacgggatgc gatcgtacag cgcctggaaa
     1261 aactcggaca gcagcgccaa acgcttgttg tcacactcga tggaaggatc caacatgcag
     1321 agtaaccctt cccgacacac atccggcctt gtttccaggc ggagttttct gcgctatgcc
     1381 agcggaacgg ccgcgttgat cgctgccggc atgataccgg tgcccgcttt tgcaaggacc
     1441 gattcgatca aattaacgat cctgcacacc aatgatgtcc acagccggat cgatccgttt
     1501 ccggacaacg accccaagta tccggggttg ggcggtttcg cgcgcaggtc agcattgttg
     1561 cggcagatcc gttcagagga atcgcacgta ctgctgctgg atgccggcga tatttttcag
     1621 ggtacaccct atttcaacct atataaagga gcacttgagc tgcaactcat gagtgaaatg
     1681 gggtatgatg ccgtcacgat cggcaatcac gatttcgatg gcggtatcga caatttgcgg
     1741 gaacgcatcg aacaggatgc cagatttccg ttcctgaacg cgaattacga gctacaggac
     1801 acgccgttga acggacgggt actcccctac aaaattttca ggcgcggagg attaaagatc
     1861 ggcgtactgg gcatcggcat cgaactgcag ggactggttg acgcgcggat gtatgggaac
     1921 gcacgctacc tggatccagt gtcgattgcc gagcaaacag cccggcgatt aaaacacgat
     1981 gaaaaatgcg acctcgtgat cgcgctgtcg catttgggtt accgttacaa ggaaaagaaa
     2041 gtctccgatg tcatactggc cgaagaaacc tcgagcattg acctgatcat cggaggacac
     2101 acgcatacat tcctcgacga gccggtacaa ttgtcgaaca aagccgggaa gaccgtgcat
     2161 gtcgcacaag tcggatgggc tggcattcgt ctcgggagaa tcgactactt ctttgatcgt
     2221 gatcgaaaga aaagttcgct cctcggcgga caagtaaaac tttctgatta cacaatagga
     2281 gcataaattt tttttaaata gtttttctac aaacctttgt tacgttattt tcgtcacggg
     2341 aaacagagaa cgaaacgaac gggacaagaa cacacgaaac gcattacccg cttaacggcg
     2401 gcacatcacg acggattcga ttttacgcac cacacaaaaa ccacttttct tagaggagac
     2461 cactatggag aaaacctgcg agagtgtttg gaaaaaatgc ctgcagatca ttaaggacaa
     2521 cgtcagcccg caaagcttca agacgtggtt cgaaccgatc aagccgatcc gtctggacgg
     2581 caaggttctg accattgaag ttccgagcca gtttttctac gaatggcttg aagagcacta
     2641 tatcaccctg atcaagaaag tcatccgcca gcaacttgga cccgagggtc gactggagta
     2701 cagcattgtg atggaacacg ccgtggctga aaagccttac accgtgcgca tcccgaccaa
     2761 gacctccggt gacctgaaga attctccggt cagtgttccg gtgaacgtga gcagcacgat
     2821 ccgcaatccc ttcatcatcc ccggattgaa gaaggtgaat gtggagtccc aactcaatcc
     2881 gaattacacc tttgagaatt tcgtcgaggg cgattgcaac cgtctggccc gttcggccgg
     2941 atacgcggtt gccggcaaac ccggcggaac cgctttcaac ccactcctta tatatggtgg
     3001 cgtcggaatg ggcaagaccc acctggcgca cgccatcggt atcgagatca agaacagcca
     3061 tcccgggaag accgtactgt atgtgacaag tgagaagttc accctgcagt tcatcgacgc
     3121 cgtccgcaac aacgaacaga acgatttcgt ccatttctac cagatgatgg atgtactgat
     3181 catcgacgat atccagttcc ttgccggcaa ggaaaaaacc caggatgtct tcttccacat
     3241 cttcaatcat ttgcaccaaa ccgggaagca aatcatcctc acatcggaca aggcgccggt
     3301 cgatctccag ggaatggagc agcgcctgct gagccggttc aagtggggtc ttgcggccga
     3361 tctgcagatg cctgacctgg aaacccggat ttccatcctc aaccggaaga tgtaccagga
     3421 cggcattgaa ttgccgaaag agattgtcga gtacatcgct tacagcatca cctcgaacgt
     3481 acgcgaattg gaaggcgccc tgatctcgat cctcgcccag gcttcgctta acaagaagtc
     3541 ggttacgctg gagctcgcca agcagatgat cgacaagttc gtcaagagca ccacgcacga
     3601 agtctcgatc gactacatcc agaagatcgt ctgcgattat ttcgacctgc ccatcgagat
//
LOCUS       DAOTON010000192         3652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20173_length_3652_cov_3.227968, whole genome shotgun sequence.
ACCESSION   DAOTON010000192 DAOTON010000000
VERSION     DAOTON010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20173_length_3652_cov_3.227968"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(594..1058)
                     /locus_tag="P5292_04940"
     CDS             complement(594..1058)
                     /locus_tag="P5292_04940"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HRS38508.1"
                     /translation="MPQRLSAVLLTLSLLFSCSKNSPTHAGGGLDSLLATDRAASRMA
                     ADSGFSATLLSYADNGFVKLNHGEYPVVGKQAFREKLNGRAGTRDISWEPRDGAVAQS
                     GDLGFTWGDWTYHGEDTTVYGNYITVWSKQADGTWKMRLDGGNETPGPPEGN"
     gene            complement(1474..2298)
                     /locus_tag="P5292_04945"
     CDS             complement(1474..2298)
                     /locus_tag="P5292_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38509.1"
                     /translation="MLRVLRLFLPALLLVCGLTASAQDLIIRTNNDTLRAKVLDMGPT
                     KIKYRIGNAKSAPVLEIYKNEVKEIIFESGSRLVVVYNRYEVPQELFVEERKAAFKVD
                     FLSSLFNHYVIGYERKWKNETNLEFKAGYIGPGISTAIREGRGYIVKAGIKFVWPEEQ
                     IMKGLRFTNAFNGKYLRPEIIFNQYSQSDESYPNRLYFTNAVINLCFGRQHVFRNKIL
                     LDYYAGFGYGWQSNNYRSTSTYDRDVVDLNYVYSHIFLGRNLPLVVSGGMMIGFCF"
     gene            2477..3340
                     /locus_tag="P5292_04950"
     CDS             2477..3340
                     /locus_tag="P5292_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRS38510.1"
                     /translation="MFNFLDKIRENPDYYRQLSVDEQLITLYDCPLEEQKVDLWSNQH
                     YFVYVVDGKKTWHANGTALELSKGSCIFVREGAHIVEQFFETRFCVVLFFLSEGFIRE
                     TIRSVAGMTPCALGRELPPILPVAADDAIHAFFQAVVPYFHQAQQVNNSLLELKFREL
                     ILQVVGNPANKDIADFFRSLEADEDTVRFRRILEENFRYNLRLEDYARLSGKSLSSFK
                     RCFEERFGVPPGRWLLERRLQHARTLLVGTRKPVSEIAWESGFESASHFSRAYRQFYG
                     HAPSEQRELTA"
     gene            3421..>3652
                     /locus_tag="P5292_04955"
     CDS             3421..>3652
                     /locus_tag="P5292_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRS38511.1"
                     /translation="MTTQTTPIAFSGQIPGNYDRYLGPMFFEPFALAIADRIGSLAPK
                     SVLELAAGTGRLTRLLPAVAGKQARILASNLNT"
BASE COUNT          768 a         1088 c          957 g          839 t
ORIGIN      
        1 tagcactcta cctcgcatct cgctccttct ttcctcgtcc cacgtccctc gtcccttctt
       61 tccttccggc attgtgtatt cgaactcccg ctatctgccc cgcaggggcg taacaaaagt
      121 ggattcaaag ctgacagtag caaagtaaga ttcaggatgt tgtcagagga tagcgaacag
      181 cgaacagtag gcggtatgca gttggcagta tgtaacacgt ccctcgtccc acgtcccacg
      241 tcccttcttt ccttccggca ttgtgtattc gaactcccgc tatccgcccc ggaggggcca
      301 aacatatttg taaaacaaat aatcgatgtt attcagcccc ggaggggcgt aacaacagtg
      361 gattcaaagc tgacagcagc aaagtagcat tcaggaggtt gtcaggggat tgtgaatgac
      421 gaatagtgaa tagtgaacag taaacagtga acagttggcg gtatgccgta tgcagttggc
      481 agtatgtaac acccctcgtt cctcgcacct cgcatctcgc acctcaattc cacgcccctc
      541 gtcccacgcc cctcgtccct tctatcctcg gtctcggtct cgttcctcgc atctcaatta
      601 ccctccggcg gccccggcgt ttcattgccg ccgtcgagcc gcatcttcca ggttccgtcg
      661 gcttgcttcg accagacggt gatgtaattc ccgtacacgg tcgtgtcttc gccgtgataa
      721 gtccagtcgc cccaggtgaa tccgagatcg cccgattggg cgactgctcc gtcgcgaggc
      781 tcccaactga tgtcgcgggt gccggcacga ccgttcagct tttcccgaaa cgcctgtttg
      841 ccgacgaccg gatattctcc atgattcaac ttgacaaaac cattgtcggc atacgacaat
      901 aacgtagcgc taaagcccga gtccgccgcc atgcgcgaag ccgcgcggtc cgttgccagc
      961 aacgaatcca atccgccgcc ggcgtgggta ggagaattct tgctgcatga aaagagaagc
     1021 gacagcgtga gtaaaactgc ggacagtcgt tggggcattg ttagttggtt tatataatca
     1081 gcgtttaaca tttgtcagag aaagggtgtc gtcttttctt tcagttgaat cattttctcg
     1141 tttatgtttt atacaccttt ccattcatcg accaatatta cgctcctccg gagctgtcga
     1201 ttagcgaagg gaaccgcacg gtctataatg ttgggcccct ccggggctgg cttttgggtc
     1261 ggcggaatgc ggtaggcaga atacggtagg cagaaagcgg ttggcagtag gcggtatttc
     1321 cctcggtctc ggtcccgttc ctcgcaactc cctttccctc gcacctcgca tcacgaacct
     1381 cgtacctaat ttccctcggt ctcggtcccg ttcctcgctc cttcttttcc tcgcccctcg
     1441 tcccccgtcc ctcgtcttta acaaccgttc ctcctaaaaa caaaaaccta tcatcatccc
     1501 cccgctcacc accagcggca agttgcggcc gaggaaaatg tgggagtaga cgtagttcag
     1561 gtccaccaca tcgcggtcgt aggtggaagt ggagcggtag ttgttcgact gccagccgta
     1621 gccgaagccg gcgtaatagt cgagcaggat cttgttgcga aagacgtgct gccggccgaa
     1681 gcagaggttg atcaccgcgt tggtgaaata caaacggttc ggataacttt cgtcggactg
     1741 cgagtactgg ttgaagatga tctccggacg caggtacttt ccgttgaacg cgttggtgaa
     1801 gcgcagccct ttcatgatct gctcctcggg ccagacgaac ttgatgccgg ccttcacgat
     1861 ataaccgcgc ccttcgcgga tcgccgtcga gatgcccgga ccgatatagc ccgccttgaa
     1921 ctccaggttg gtctcgttct tccatttgcg ttcgtacccg atcacgtagt ggttgaacag
     1981 tgatgaaaga aaatcgacct tgaaggccgc cttgcgctct tccacgaaaa gttcctgcgg
     2041 cacttcgtag cggttgtaca ccaccaccag gcgggaaccg ctctcgaaga tgatctcttt
     2101 cacttcattc ttgtagatct ccagcaccgg cgcgctcttc gcgttgccga tccggtactt
     2161 gatcttcgtc ggacccatgt cgagcacttt cgcacgcagg gtgtcgttgt tggtacggat
     2221 gatcaggtcc tgagcagagg ccgttaaccc acacacgagc aatagagcag gcaggaaaag
     2281 gcggaggacg cgtaacaagg acatgcgggg ccggtgcggg aagaggttaa aaatcgagta
     2341 caaactaaga aaaaacatga aatcgatgtg tgagaaccat ttttaacaga caaaacagta
     2401 cgacgaaagg atgaggcagg attaaagcgg cagtgcatat ccgttcgact cgtactttta
     2461 cagcattttc ctccccatgt tcaacttttt agataagatc cgggagaatc ccgactacta
     2521 ccgtcagctc tccgtcgacg aacagttgat cacgctgtac gactgtccgc tcgaggagca
     2581 gaaggtcgat ctctggagca accagcacta tttcgtgtat gtggtcgacg gcaaaaagac
     2641 ctggcacgcg aacggaaccg cgctcgaact ctcgaaaggc agttgcattt tcgtgcgcga
     2701 aggtgcacac atcgtcgagc agttttttga gacgcgcttt tgtgttgtgc tgttctttct
     2761 cagcgaaggg ttcatccggg aaacgatccg gagcgtcgcg ggcatgacgc cctgcgcgct
     2821 cggacgcgag ctgcccccga tcctgccggt cgctgccgat gacgccatcc atgctttctt
     2881 ccaggcggtc gtgccgtatt tccaccaggc gcagcaggtg aacaactccc tgctcgaact
     2941 caagttccgc gaactcatcc tgcaggtggt cggtaacccg gcgaacaagg acatcgccga
     3001 ctttttccgt tcgcttgaag ccgacgaaga caccgtgcgt ttccggcgga tactcgaaga
     3061 gaactttcgc tacaacctcc ggctcgaaga ctacgcccgc ctcagcggca agagcctgtc
     3121 gtcgttcaag cgctgcttcg aagaacgctt cggcgttcca cccgggcgct ggctgctgga
     3181 gcgccgcttg cagcacgcgc gtacgctact cgtcggcacg cgcaagccgg tgagcgagat
     3241 cgcctgggag agcggcttcg agagcgcttc gcatttcagc cgcgcctacc gacagttcta
     3301 cggacacgcg ccttcggaac agcgcgaatt gacggcctga gccgcacagg caattctttg
     3361 gactttctgg caacgtccgc gcgcggaggg cgctttagtt ttgccgtaaa tattttcatc
     3421 atgactaccc aaacaacccc cattgcattc tcgggtcaga tcccgggaaa ttacgaccgc
     3481 tacctcggtc cgatgttctt cgaacccttc gccctggcca tcgccgatcg catcggcagc
     3541 cttgcgccga agtccgttct cgaattggcc gccggcaccg gtcggctcac ccgcctgctg
     3601 cccgcggtcg ccggcaagca agcgcgcatt ctcgcttcca atctgaatac cg
//
LOCUS       DAOTON010000193         3634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20303_length_3634_cov_3.528080, whole genome shotgun sequence.
ACCESSION   DAOTON010000193 DAOTON010000000
VERSION     DAOTON010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20303_length_3634_cov_3.528080"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="P5292_04960"
     CDS             <1..249
                     /locus_tag="P5292_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018676314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRS38512.1"
                     /translation="AVLGANVVLTMSTKIIDVTGSEPVEYKGYVPPRSVVIPGSYTKR
                     FPAGEYQVPCALIIGQRKESTDKKTSLNDALRDHSVAV"
     gene            251..1234
                     /locus_tag="P5292_04965"
     CDS             251..1234
                     /locus_tag="P5292_04965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRS38513.1"
                     /translation="MRSCLIIQTAFTGDVILATAVAEKLHTAFPGMPIDFLVRKGNEG
                     LLEHHPYIREVLVWNKREHKTKHLIATARSVRSRKYELVVNLQRFAGSGFISAYSGAR
                     YRSGFDKNPLAITYTDRHRHVIGDGRHECERNQQLIARWTDDQPALPRLYPNDSDFAA
                     IRRFTERPFVCIAPGSVWFTKTWPAAQWIKLIQLLLHREPELRVFLLGAPGEHTLAES
                     IRLSSESGQVHNLAGELSFLESAALMSKAGMNYVNDSAPLHLASAMNAPVTAVFCSTV
                     PEFGFGPLSTRQRVIQTSRQLSCRPCGIHGFRACPKGHFQCATTIDPQELL"
     gene            1231..1818
                     /locus_tag="P5292_04970"
     CDS             1231..1818
                     /locus_tag="P5292_04970"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRS38514.1"
                     /translation="MTNRFSPDEEQILRKAVDVLKSGGLILYPTDTIWGLGCDASRED
                     AVEKLIRLKGKEEGQGLIVLLDDVNRVERYTRAVPAVAYDLMELSEKPLTLVLDEGMM
                     VASAVLGPESSIAIRVTQDPICRELVRRLRKPLVSTSANLAGGPSPACFADITDSLKS
                     GVDYILPLRQAEKKPPVASTVIRLKADGQIRILRK"
     gene            1851..3287
                     /locus_tag="P5292_04975"
     CDS             1851..3287
                     /locus_tag="P5292_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRS38515.1"
                     /translation="MNVPQLPVFRIVAEEAQAMGTEAFVVGGYVRDLILQRESKDIDF
                     VVLGNGIDLAQRVAKRLGHHVPVNIFKNFGTAMIRNGEWEIEFVGARKESYTRDSRKP
                     AVETGTLEDDQKRRDFTINAMALRLQPDGSTELLDPFNGIQDLQDKLIRTPVDPDLTF
                     SDDPLRMLRAARFATQLGFRIGDNEFSAISRNKERIRIVSMERISDELNKIIQAKKPS
                     IGFDLLFRSGLLQEFFPQLCLLHGVEYVNGKGHKDNFYHTLQVLDNVAEMSDDLWLRW
                     AAILHDIAKPATKRFEPDHGWTFHGHEDRGARMVKHIFRQLKLPLNEKMKFVEKLVQL
                     HLRPIVLAQEHVTDSAIRRLLFDAGDDIDALMILCKADVTTKNDYKIRKYRENFEIVQ
                     QKLREVEEKDRIRNWQPPVTGEHIMEAFTLQPGREVGLIKTAIREAILEGDIHNEFNE
                     AYEFMLRKGRELGLNGSDRRTATGAADA"
     gene            3346..>3634
                     /locus_tag="P5292_04980"
     CDS             3346..>3634
                     /locus_tag="P5292_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38516.1"
                     /translation="MAIYRFRVTFEDYDDVYRDIEVRSNQTFEDLHHAIQSAIGFDAS
                     KPASFYMSNDNWTKGREITSRELSEEQAAQIASLRKARLCDYIADPHQKIYY"
BASE COUNT          886 a         1034 c          957 g          757 t
ORIGIN      
        1 gctgtcctcg gtgccaacgt ggtactgacc atgtcgacca aaatcatcga cgttacagga
       61 tccgaaccgg tggaatacaa aggctacgtt cctccccgct cggtggtcat tccagggtcc
      121 tacaccaagc gattccctgc cggagaatac caggtaccct gtgccctgat cataggacag
      181 cgcaaggaaa gtaccgataa gaaaacatcc ctgaacgacg ccctgcgcga ccacagcgtg
      241 gctgtctgat atgcgttcct gtctgatcat acaaaccgca ttcaccggtg acgtgatcct
      301 cgcgacagcg gttgcggaaa agctgcatac agcctttccc gggatgccga tcgactttct
      361 cgtgcgaaag ggtaacgagg gattgctgga acatcacccc tacatccggg aggtattggt
      421 ctggaacaaa cgcgaacaca agacaaaaca cctgattgca acggcacgtt ccgtacgttc
      481 ccgcaaatac gaactcgtgg tcaacttaca gcgattcgcc ggtagcgggt tcatctcagc
      541 ctattcaggt gccaggtacc gtagcggatt tgataagaat ccgctcgcca ttacgtacac
      601 agaccgtcat cggcatgtga tcggcgatgg tcgtcatgaa tgcgaacgaa accagcaact
      661 catcgcccga tggaccgacg atcagccggc acttccacga ctctatccga atgattccga
      721 tttcgccgcc atacgtcggt ttaccgaacg gccttttgtc tgcatcgctc ccggttcggt
      781 ctggttcacc aagacatggc cggccgcgca atggatcaaa ctgatccaac tactcctcca
      841 tcgcgaaccg gaactgcggg tcttcctgtt gggtgctccc ggggaacata cgctggcgga
      901 atcgattcgt ctgtcaagtg aaagtggtca ggtgcataac ctggccggag aactttcgtt
      961 tctggaatcg gcggcgctga tgagcaaagc cggcatgaat tacgtcaacg attccgctcc
     1021 cctccacctt gccagcgcaa tgaatgcacc cgtcaccgcg gtattttgtt ctacggtccc
     1081 ggaattcggt tttggcccgc tgagcacccg acagcgcgtg atccagacgt cccggcagtt
     1141 aagctgccgg ccctgcggca ttcacggatt ccgcgcttgt ccgaaaggcc atttccaatg
     1201 tgcaacaacc atcgatccac aggaactcct atgacgaatc gattttcacc agacgaagaa
     1261 cagatcctcc ggaaagcggt cgatgtacta aaaagcggcg gactgatcct ctacccgact
     1321 gataccatct gggggctggg ctgtgatgct tcgcgggagg atgcggtaga aaagctcatt
     1381 cgactcaagg gaaaagaaga aggccaggga ctgatcgtct tactcgatga tgtcaaccgc
     1441 gtagaacgtt acacacgcgc tgttccagca gtagcttatg atctgatgga gttgtcggaa
     1501 aaaccgctca ccctcgtgct tgatgaaggc atgatggtcg catccgcggt tctcgggcct
     1561 gaatcatcca tcgcgatcag ggtcacacaa gatccgatct gccgggagct ggtacgacga
     1621 cttcgcaaac ccctggtttc aacttccgcc aatctggccg gaggaccgtc tcccgcttgc
     1681 tttgcagata tcacggacag cttgaaatcg ggcgtggatt atatcctacc tttgcggcaa
     1741 gctgagaaaa aaccgccagt ggccagtacg gtcattcgtc tcaaagccga tggacagatc
     1801 cggatcctcc ggaaataatg cccgacggca gccctgcaag gactcccgcc atgaatgttc
     1861 cccaactgcc tgtattccgc atcgttgccg aagaggccca ggctatgggt accgaagcct
     1921 ttgtggtagg cggatatgtt cgcgacctga tactccaacg cgaatcgaag gatattgatt
     1981 ttgtcgtcct tggaaatggc atcgatctgg cacaacgcgt cgccaaacga cttggtcacc
     2041 atgttcccgt aaacatcttc aagaatttcg gtaccgccat gatccggaat ggcgaatggg
     2101 aaattgaatt cgtcggcgcc cggaaagaat cctatacgcg ggactcccga aaaccggctg
     2161 tcgagacggg cacgctggag gacgatcaga aacgtcgcga tttcaccatc aatgccatgg
     2221 ccttgcggct gcagccggac ggctctaccg aactgctcga tcccttcaac ggaatacagg
     2281 acctgcagga taagctgatc cgcaccccgg tggatcccga ccttacgttc agcgatgacc
     2341 cgttgcgcat gctccgcgcg gcacgattcg cgacgcaact cggttttcga atcggagata
     2401 atgagttctc cgcgatcagc cggaacaagg aacgcatccg gattgtttcc atggaacgga
     2461 tcagtgatga gctcaacaag atcattcagg caaagaagcc ctccatcggc ttcgacctgc
     2521 tcttccgctc cggcctttta caggaattct tcccgcaact ctgcctgtta cacggagtgg
     2581 aatacgtaaa cggaaaggga cacaaagaca acttctacca taccctgcag gtgctcgaca
     2641 atgtggcgga aatgtcggac gacctttggc ttcgctgggc cgccattctc cacgacatcg
     2701 ccaaaccggc caccaagcgt ttcgaaccgg accatggctg gacctttcac ggacacgaag
     2761 accgtggtgc acggatggtg aagcacatct tccgtcagtt gaaactgccg ttgaacgaga
     2821 agatgaaatt cgtggagaaa ctggtgcagt tgcatctacg gccgatcgta ctggcccagg
     2881 agcatgtcac cgactcggcg atccgacgct tgctattcga tgcaggggat gatatcgacg
     2941 cgctgatgat cctgtgcaag gcagacgtca ccaccaagaa cgactacaag atccggaagt
     3001 accgggagaa tttcgagatc gttcagcaaa agttacgcga agtcgaagag aaggaccgca
     3061 tccggaattg gcaaccaccc gtgaccggtg aacacatcat ggaagccttt acccttcaac
     3121 cgggtcggga ggtcggactc atcaagacag ccatacgcga agcgatcctg gaaggcgaca
     3181 ttcacaatga gttcaacgaa gcctacgaat tcatgctgag aaaaggtcgg gaactgggcc
     3241 tgaatggaag cgaccggagg accgccactg gtgcggctga cgcatgactt ttagcgtcgt
     3301 gcgaatggaa ttcttttttt atttttgagt actgaattac cacgtatggc catctaccgc
     3361 ttccgggtca cctttgaaga ttacgatgac gtttaccgcg acattgaggt ccgttccaac
     3421 cagacattcg aggacctcca tcacgcgatc caaagcgcca tcggattcga cgcgtccaag
     3481 ccggcctcgt tctacatgag taacgataac tggacgaagg gacgggaaat cacatcacgc
     3541 gagctttcag aagagcaagc tgcccagatc gccagtttgc ggaaggccag actttgcgac
     3601 tatatcgcgg acccgcatca gaagatttac tacc
//
LOCUS       DAOTON010000194         3626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20356_length_3626_cov_2.785774, whole genome shotgun sequence.
ACCESSION   DAOTON010000194 DAOTON010000000
VERSION     DAOTON010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20356_length_3626_cov_2.785774"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2595)
                     /gene="secA"
                     /locus_tag="P5292_04985"
     CDS             complement(<1..2595)
                     /gene="secA"
                     /locus_tag="P5292_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRS38517.1"
                     /translation="MLGILNKTIGKIFGSKRDRDLKELLPIVDLAKAEFSKLSAISND
                     ELRSRTSDFRRRIEDHLKETNEEIKRLETEAAGLIDGDIVRQEEIYREIDQLKKQRNK
                     ETEEVLMDLLPEAFAVVKETARRFSEQAEIVSTATELDRNLATRKDYVRIDGDRSVFK
                     NSWSAAGNVVTWNMVHYDVQLIGGLVLHQGKISEMATGEGKTLVGTLPIYLNALASQG
                     VHVVTVNDYLAKRDSEWNGPIFEFLGLTVDCIDKHDPNTPARRQAYLADITYGTNNEF
                     GFDYLRDNMARATEDMVQRGHHFAIVDEVDSVLIDDARTPLIISGPTPKGDHHEFDVY
                     KPKVEQLVRAQREYVNTLISDARKLLEAKKEDEAGLALLRAHRGLPKTKSLIKLLGEQ
                     GVMAIMRKTENHYMQDQSREMHKADATLYFVIDEKNNNIELTEKGVELMSSNVSEKNF
                     FVLPDVGAEIAEIERSTATPEDKITRKDDLMRDYAVKSERIHTVNQLLRAYALFEKDV
                     EYIIADGKIKIVDEQTGRIMEGRRYSDGLHQAIEAKENVKVEAATQTYATITLQNYFR
                     MYHKLAGMTGTAETEAQEFWSIYKLDVVSIPTNKPISRKDHEDKVYKTKREKYNAVIE
                     EIEQLVKAGRPVLVGTTSVETSELLSRMLKLRNIRHNVLNAKLHQKEAQIVAEAGQSG
                     TVTIATNMAGRGTDIKLGSGVREAGGLAIIGTEKHESRRVDRQLRGRSGRQGDPGSSQ
                     FYVSLEDDLMRLFGSERIMKLMDRMGIEEGEVIQHSMITSSIERAQRKVEENNFGIRK
                     RLIEYDDVMNAQREVIYTRRRHAIFGERLSIDINNMLYDTCESIVQQFQESRDYEGPK
                     LDLVRILSI"
     gene            2751..>3626
                     /locus_tag="P5292_04990"
     CDS             2751..>3626
                     /locus_tag="P5292_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38518.1"
                     /translation="MRILRLYVWSALLTGLLFPKENSAQPWMKRPLTSGDEVVRKRPN
                     LQDLSARFNEYWNSRTPSIDEGENAEEGGYQQFRRWEWFMKQRTWPSGDLPDPNILFR
                     EWKRTRDVRPVNNARMQSTNWTYVGPDVVPANGGGAGRLNVLRFNPVDNNILYLGSAS
                     GGLWKSIDGGSTWTALDDFLTALSVADIAINPRYPDSIYVATGDGYGYEVNGDFWGGT
                     YSAGILVSPDGGVTWNPTGIAFTQDQNNIVQRLTINPQDPSIMLAATRYGLYRSTDAG
                     VNWNSVRTSKHYDIEW"
BASE COUNT          715 a          987 c          994 g          930 t
ORIGIN      
        1 gatcgaaagg atccggacaa ggtcgagttt cggaccctcg taatcgcggg attcctggaa
       61 ttgctgtacg atcgactcgc aagtgtcgta caacatgttg ttgatgtcga tgctgagccg
      121 ctccccgaaa atggcatgac gccgacgggt gtagatcacc tcccgctgcg cgttcatgac
      181 gtcgtcgtat tcgatcaggc gcttccggat accgaagttg ttctcttcga ctttgcgctg
      241 ggcgcgctcg atggagctgg tgatcatcga gtgctggatg acttcccctt cctcgatgcc
      301 catgcggtcc atcagcttca tgatccgttc cgaaccgaag agtcgcatca gatcatcctc
      361 aagcgaaaca tagaactgcg agcttccggg atctccctga cgtccggaac gaccgcgcaa
      421 ctgtcggtcg acgcgacggg attcgtgctt ttcagtaccg atgatggcaa gaccgcccgc
      481 ttcgcgaacg cccgatccga gcttgatatc ggttccgcga cccgccatgt tcgtggcgat
      541 ggtcaccgtg ccggactgtc ccgcttcggc aacgatctgg gcttcttttt ggtggagttt
      601 cgcgttgagt acgttgtggc ggatgtttcg cagcttgagc atacggctca gcaattcaga
      661 agtctcgaca ctggtcgtac cgaccaatac cggccggccg gctttcacca attgttcgat
      721 ctcttcgatc accgcgttgt atttctcccg cttggtcttg taaaccttgt cttcgtgatc
      781 cttacgggag atgggcttgt tcgtcggaat gctgacaaca tccagtttat agatcgacca
      841 gaattcctgt gcctccgttt ctgccgttcc ggtcatgccg gccagcttgt ggtacatccg
      901 gaagtaattc tgcagcgtga tggtggcgta ggtctgcgtc gcggcttcga cttttacgtt
      961 ctcctttgct tcgatggcct ggtgcagtcc gtcggaataa cgccggcctt ccatgatacg
     1021 accggtctgt tcatcgacga tcttgatctt gccgtcggcg atgatgtact cgacatcttt
     1081 ctcgaacagg gcataagcgc gcaggagctg gttgacggta tggatacgct ccgacttgac
     1141 cgcgtaatcc cgcatcaggt cgtctttccg ggtgatcttg tcttccggcg tagcggtcga
     1201 tcgttcgatc tcggcgattt cagcgccgac gtccggtagt acgaaaaagt tcttctccga
     1261 aacattggac gacatcaact cgacaccttt ctcggtgagt tcgatgttgt tgttcttttc
     1321 gtcgatcacg aaatacaggg tcgcgtcggc tttgtgcatc tcgcgcgact ggtcctgcat
     1381 gtagtgattc tccgtcttgc gcatgatggc catgacgcct tgttcgccca gcaacttgat
     1441 cagggatttc gttttgggca atccgcgatg cgcgcgcaac agggcaaggc ccgcttcgtc
     1501 ttctttcttc gcttcgagta atttccgcgc gtcggaaata agtgtattca cgtactcacg
     1561 ctgagcacga acgagctgtt cgactttggg cttataaacg tcgaattcgt gatgatcacc
     1621 tttgggcgtc ggtccggaga tgattagcgg tgtccgggcg tcgtcgatga gtaccgagtc
     1681 cacctcatcc acgatcgcga agtggtggcc gcgttgtacc atgtcttccg tagcacgggc
     1741 catgttatcg cgcaggtaat caaagccgaa ttcgttgttg gtgccgtagg tgatgtcggc
     1801 aaggtaggct tggcggcggg ctggcgtatt ggggtcgtgc ttgtcaatgc agtcgaccgt
     1861 caatccgagg aattcgaaaa tcggtccgtt ccattccgag tcgcgttttg cgaggtaatc
     1921 gttcacggtc acaacgtgaa cgccctgact cgccagggcg ttcaggtaaa tgggcagggt
     1981 gccgacgagc gtttttcctt caccggtcgc catctccgaa atcttaccct ggtggaggac
     2041 caaaccgcca atcaactgca cgtcataatg aaccatgttc caggtcacca cgttgccggc
     2101 agcagaccag ctgttcttga aaaccgaccg atctccgtcg atgcgcacat agtccttgcg
     2161 ggtggccaga ttgcggtcca gttccgtggc ggtcgatacg atctctgcct gttcactgaa
     2221 cctgcgggcg gtttctttca ccacagcaaa agcctcgggc aagaggtcca tcaggacttc
     2281 ctccgtttcc ttgttccgct gcttcttaag ttggtcgatt tcccgataga tctcttcctg
     2341 tcgaacgata tcaccgtcaa tcagtccggc cgcttcagtc tccaggcgtt tgatctcttc
     2401 attcgtttcc ttcagatgat cttcgatccg tctccggaaa tcggaagtcc tggaccgcag
     2461 ctcatcgttc gaaatggcgg aaagcttact gaattcggcc ttcgccaaat ccacgatcgg
     2521 aagcagttct ttcaggtcgc ggtcacgctt gctgccgaag atctttccga tggttttatt
     2581 gagaattcca agcattatac aggtagggta aaaggaggac aaaggtactc tttatttccg
     2641 gctttggcgg aaggcattcg atgtcaatca attgtcatct ttgagcagag ccggcagcag
     2701 gctggtgcag gtgaggcggt atttctatct ttgaaaaaat accgctttac atgcgtatcc
     2761 tgcgcctata tgtctggtcg gctttgctca ccggattatt gttccccaaa gaaaattccg
     2821 cacaaccctg gatgaagcgt ccgctgacca gcggcgatga agtggtgcgc aaacgtccca
     2881 accttcagga tctttcagca aggttcaacg agtattggaa ctccaggacc ccttccattg
     2941 atgaagggga gaatgcggaa gaagggggct atcaacagtt ccgtcgctgg gaatggttca
     3001 tgaagcaacg tacctggcct tccggtgatc tgccggatcc gaatatcctg ttccgggagt
     3061 ggaagcggac ccgtgatgtc cgcccggtca ataacgcgcg gatgcaatcg acgaattgga
     3121 cctatgtcgg accggatgtg gtgcccgcca atggcggagg agcaggtcgc ttgaatgtgt
     3181 tgcgcttcaa tccggtcgat aataacatcc tttacctggg atctgccagc ggcggtctgt
     3241 ggaaatccat cgacggcggc tccacctgga cggcgctcga cgatttcctc acggcgcttt
     3301 cggttgccga tatcgccatt aatccccgtt acccggattc gatttatgtc gcaaccggcg
     3361 acggttacgg gtatgaggtc aacggtgatt tctggggagg aacgtacagt gccggcatcc
     3421 tcgtttcacc ggatggaggc gttacgtgga atccgaccgg aatagccttt actcaggatc
     3481 aaaacaacat cgtccagcgg ttaaccatca atccgcagga tccttcgatc atgctggctg
     3541 ccacccgata tggtctgtat cgttcgaccg atgcgggagt caactggaac tccgtccgta
     3601 catccaagca ttatgacatt gaatgg
//
LOCUS       DAOTON010000195         3624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20368_length_3624_cov_2.824881, whole genome shotgun sequence.
ACCESSION   DAOTON010000195 DAOTON010000000
VERSION     DAOTON010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20368_length_3624_cov_2.824881"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="P5292_04995"
     CDS             <1..524
                     /locus_tag="P5292_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS38519.1"
                     /translation="PLFEEIGLFDEELVRNQDDKFNGRIIRSGGKILLLPDLKIRYFA
                     RERLSKIRRMFYQYGLFKPLVNRKLGKPATVRQLFPPLLVAGIMLPPAIAYWIPMIGL
                     CWRVGLMAYLMMVVYFSLRLSNGNGSLLGALIRVFPTIHLSYGIGYWIGIIRFLIFRK
                     NQHGKVHDPGISR"
     gene            490..1812
                     /locus_tag="P5292_05000"
     CDS             490..1812
                     /locus_tag="P5292_05000"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRS38520.1"
                     /translation="MEKYTIRESVVDSPTFSNMKQDTPTVQPIPNAPWRRLKLWALPL
                     LALTLPFGHGAVTVAMVIVLLTTILNSNAQPWIKEFRLWYLLPVAFLVLHVVGMAYSD
                     NRSFGLFDIQVKSGLLILPICVMMHGRLSAQDWQRFVRAFLAGLLLAFVACLLPAIRT
                     WSATGSLHGFFYQDFSRFLHPSYFAWYADTAVLLLWLNRADPILKGALRRSLLLMLSL
                     LVFLCNSKAGFAGWFFVTALGALVTGLKTKSFRLFVVSLLHILIFGLAIWKVIPPEEN
                     RVMAMQQNLSQDTISGSDGRSTSARLHVWSAAWSLIQEAPVTGYGTGDVKDVLLKRYA
                     ERGLISAVEKKLNAHNQFLQSWVALGLSGVLLLLAMLAGATWRFRRDPLFAGFLLLTF
                     FNFLVESMLEIQAGVVFWALFYSVFLNLQSPEGSYEDHQPGRRPSPVH"
     gene            1769..2881
                     /gene="wecB"
                     /locus_tag="P5292_05005"
     CDS             1769..2881
                     /gene="wecB"
                     /locus_tag="P5292_05005"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRS38521.1"
                     /translation="MKIINLVGARPQFIKAAMLSRALRRQRGVEEIMVHTGQHYDASM
                     SDDILKELEFPVIAHNLGINRLSHGAMTGRMIESLEQVFQQERPDALIVYGDTNSTLA
                     GAVAAVKLGIRIAHVEAGMRCNNLHVPEEANRVVADRLSEWLFTSSDHARQNLIREGY
                     DNLPVQLIHSGDVMLDAFRHFQPIAQERISWPQEMPQGDFVLCTVHREATTEHPGRLR
                     EVFEAIERVHQRIPVVLPLHPRTRERIGALGIKTSAVLTEPASYLQMLVLLDRCALVM
                     TDSGGLQKEAFFSKKPCVTLRPETEWIELVEHGYNKVVGTDPQWIVNHVFWMLDNPPE
                     FRHHFYGDGHAADLIAAQLIKPSIHSVRKDQPTNLA"
     gene            2878..>3624
                     /locus_tag="P5292_05010"
     CDS             2878..>3624
                     /locus_tag="P5292_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HRS38522.1"
                     /translation="MIPFSPPRIDQAIVDEVVAALRSGWITTGPRTKRFEQELAAYCK
                     VPAVLCLNSATAGLELMLRWFGVGPGDEVILPAYTYSATANVVVHCGATPVFTDVRDD
                     FNMDPEAVAVAINERTKVIMPVDFGGWPCDYRALNELVRQPDVRAKFRPNSNVQATLG
                     RILVLSDSAHSLGAWYHGQRAGSLTDCSVFSFHAVKNLTTAEGGAVALNLPEGFDNTA
                     IYQELCVKSLHGQNKDALAKMQKGNWRYDIV"
BASE COUNT          751 a          939 c         1005 g          929 t
ORIGIN      
        1 gaccgctttt tgaggagatc ggattgtttg acgaggagtt ggtccgaaat caggacgata
       61 aattcaatgg aagaatcatc cgttccggag gtaagatact tttactgccg gaccttaaga
      121 tccggtattt cgcccgtgaa cgtctgtcca agatccgccg gatgttctac cagtacggtt
      181 tattcaaacc gcttgtcaac cggaaactcg gaaaaccggc gactgtccgt cagctctttc
      241 ctccattgct ggtggccgga atcatgttgc ctcccgcgat cgcatactgg attccaatga
      301 tcgggttgtg ctggcgggtt gggttgatgg cgtatctcat gatggtcgtt tatttttccc
      361 ttcggctttc caatgggaac ggctctttgc tgggggcgct cattcgggtc ttcccaacca
      421 ttcacctgag ctatggaata gggtattgga tcggtatcat acgattcctg atcttccgga
      481 aaaatcaaca tggaaaagta cacgatccgg gaatcagtcg ttgatagccc cacattcagc
      541 aatatgaaac aggatacacc gacggtacag cctataccca acgctccctg gcgccggctg
      601 aaactctggg ctttgccttt gctggcgctg accctgccgt tcggacatgg cgccgtcacg
      661 gtcgcgatgg tgattgtcct gttaacgacg attttgaata gcaatgctca gccgtggata
      721 aaggaatttc ggttgtggta cttactgcca gtcgcctttc tggtgttgca tgtagtaggc
      781 atggcttact ccgacaatcg ctcgttcggg ctctttgaca tccaggtaaa atcggggctg
      841 ttgattctgc cgatttgtgt gatgatgcac ggcaggttat cagcgcagga ttggcaacga
      901 ttcgtacgtg cctttcttgc cggactgctc ttggcattcg tcgcttgtct ccttcctgca
      961 atccgtacct ggagtgccac cgggtcgctg catggatttt tttatcagga tttcagtcgg
     1021 ttcctgcatc cttcgtattt cgcctggtat gccgacacgg ccgtcttgct actctggctg
     1081 aaccgtgcgg atccgatctt gaaaggcgcc ttgcgaagaa gtctgctgct catgctctca
     1141 ctcctggtct tcctctgtaa cagtaaggct ggatttgcag gatggttttt tgtgaccgcg
     1201 ttgggagccc tggtaactgg tcttaagacc aaatcgttcc gtctcttcgt tgtgtccttg
     1261 ttacatatcc tcattttcgg actcgcaata tggaaagtca tcccgccgga ggagaatcgg
     1321 gtaatggcca tgcagcagaa cctctcccag gatacgattt ccggaagcga cggacgatcg
     1381 acctcggcgc gattgcatgt ttggtcggct gcttggtcac tgattcagga agctccggtg
     1441 accggctatg gaaccggcga cgtaaaagat gtgctgctga agcgatatgc ggaacgaggc
     1501 ctgatctccg cggtcgagaa gaaactcaat gcgcacaacc agttcttgca atcatgggtt
     1561 gcgcttggcc tttccggtgt tttgctgcta ttggccatgc tggcaggtgc aacctggcgg
     1621 tttcgaaggg atcccctctt tgccggattt ctgctactga ccttcttcaa tttcctggtg
     1681 gaaagcatgc ttgaaataca ggctggcgtg gtcttttggg cgctttttta ttccgtattt
     1741 ttgaacctcc aatctcctga aggttcgtat gaagatcatc aacctggtag gcgcccgtcc
     1801 ccagttcatt aaagccgcca tgctgagccg cgcgctgcgt cggcaacgtg gagtggagga
     1861 gatcatggtt cataccgggc aacattacga cgcctccatg tcggatgata tcctgaagga
     1921 attggagttt ccggtcatag cccataacct cgggatcaat cgactttcac acggagcaat
     1981 gaccggtaga atgatcgagt cgctggagca ggtatttcaa caggaacgcc cggatgcgct
     2041 catcgtgtat ggtgatacga acagtaccct ggctggtgcg gttgccgccg tcaaactggg
     2101 tatccggatc gcccacgtgg aagccggcat gcgttgtaac aatcttcacg taccggagga
     2161 agccaaccgg gttgttgccg accgtttgtc ggaatggtta tttacctcct ccgaccatgc
     2221 gcgacagaac ctgatccgcg aaggctacga taatcttccg gtgcaattga tccattccgg
     2281 cgatgtgatg ttggacgcgt tccggcattt ccagccgatc gcgcaggaac gtatttcctg
     2341 gccccaagag atgccacagg gggactttgt cttgtgtacg gttcaccggg aagcgactac
     2401 cgagcacccg ggcagactgc gggaagtgtt tgaagcgatc gagcgtgtgc atcagcgcat
     2461 cccggtcgtg cttccgcttc atccgcgtac ccgcgagcgt atcggtgcac tgggcatcaa
     2521 gacttccgcc gtcttaacag aacctgcatc ttatctgcaa atgctcgtgt tattggatcg
     2581 atgcgctttg gtcatgacgg attcaggagg gttgcagaaa gaagcgttct tcagtaagaa
     2641 gccttgtgtg acacttcggc cggaaaccga atggatcgaa ctggtcgaac atggttacaa
     2701 taaagtggtt ggcaccgatc cgcaatggat cgtcaatcat gtgttctgga tgttggacaa
     2761 tccgccggag ttccggcatc atttttatgg ggatggtcat gctgccgatc tgatcgcggc
     2821 tcagttgatc aagccttcca ttcattccgt cagaaaagat caacccacaa acctcgcatg
     2881 ataccctttt caccaccccg gatcgaccag gcgatcgttg atgaagtcgt ggctgcgctg
     2941 cggtcaggct ggataacaac cggcccccgc acgaaacgtt ttgagcagga gctggccgct
     3001 tattgcaaag tgccggctgt gctctgtttg aattctgcca cggccggcct ggaattgatg
     3061 ctccgttggt tcggcgtcgg tcctggcgat gaagtcattc ttcctgctta tacctatagt
     3121 gccactgcca atgtggtcgt acactgcgga gcgacaccgg tctttaccga tgtgcgcgat
     3181 gatttcaata tggacccgga ggcggtggca gtcgcgatca acgagcggac caaagtgatc
     3241 atgccagtcg atttcggcgg atggccgtgc gattaccgcg cgctgaacga gcttgttcgg
     3301 caacccgatg ttcgcgcgaa gttcaggccg aactcaaacg tgcaagcgac actcggaaga
     3361 atactcgtct tatcggattc cgctcattca cttggtgctt ggtatcacgg acagcgagcc
     3421 gggtctctca cggattgctc ggtcttctcc tttcatgcgg tgaagaatct cacgacggca
     3481 gaggggggag cggtcgcgct gaatcttccg gagggattcg ataatacggc catctaccag
     3541 gaactttgtg tcaagtccct gcacggacaa aacaaggatg cactggcgaa aatgcagaag
     3601 ggcaactggc ggtacgatat tgtc
//
LOCUS       DAOTON010000196         3618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20402_length_3618_cov_3.053045, whole genome shotgun sequence.
ACCESSION   DAOTON010000196 DAOTON010000000
VERSION     DAOTON010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20402_length_3618_cov_3.053045"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="P5292_05015"
     CDS             <1..493
                     /locus_tag="P5292_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38523.1"
                     /translation="NGYLLIWADDQNTTWIDYHTNFNLTLSGDTILLSDSLGVLADSI
                     LYQQQTQDLSASRCADGTGPFVDGTTPTPRAMNDCASTGYYVSTASDALIVYPNPASD
                     LVSIRSEKSMQRVEILDLQGQVIEQRELSDDKQVQIGLERFSPGLYLLRVDGLTPVRL
                     VIQ"
     gene            639..1019
                     /locus_tag="P5292_05020"
     CDS             639..1019
                     /locus_tag="P5292_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRS38524.1"
                     /translation="MNDITDRQDVITLVDSFYRQVREHPLLGPIFNEVAKVDWTAHLP
                     KMYSFWATLLLGENSYSGNPMLPHLDLSRKATLDEEAFNAWKQLFNQTVDSLFAGTKA
                     EEAKERAGNIARLMLHKVQSAKAY"
     gene            complement(1081..3459)
                     /locus_tag="P5292_05025"
     CDS             complement(1081..3459)
                     /locus_tag="P5292_05025"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRS38525.1"
                     /translation="MRASTDNRPRNDDEIDLRLLFSNIRRNWHYFLISLLVFGTAGLL
                     YIRFTLPVFEANTSVLIKDSKNKSSNIEDILTGDLFGNVKNVATEIGILRSRSVLQET
                     IEELGLGVSYYGKTTFFRYPIYKNNPFIVEPLYVQDGLYDEEFHIRLLDSLTYQLEIE
                     ADNRLVDDFNYSSKHRFGEEVQTARFKLRIVRNDPSFAARQDDDFRFVIQSTNKLIAW
                     YSEKMKAEPINKDATIINITIQDQVPERAVDFLNTLGKVYINQDVKDKSSVAALTLKF
                     VDEQLEEISKTLNATELELQRFKEQKGTVDLSEESKAYLERVTRVDADRAKAELELKS
                     LDYLYNYVKSNKDLDQLAPSSLGTPDPLLIDLIGQLKNLQTKRRSLRYGASENSPAIK
                     VIDQQIEETKASLIENINSLRNQTRVSLGTIQGQLAEYEGSIRKIPNIERELLGIQRN
                     FSVNENIYLYLLQKKAETGIAKATAVSDNKVLDSASFNEIPVIPNRKAVAIITLLLAL
                     VLPILFIALQGYLKNTVSNREDVEKQTKVPVIGVVGHHTSSERLVVSAKPKSAIAEAF
                     RSIRANLMFLGLADQHKIVLITSSVGGEGKSFSTLNLAAVLALQHYRVIIVGMDLRKP
                     QLVQDFGIDNSIGVSNYLIGRATLEEVIHKTSVDGLEIIPSGPVPPNPAELLANKRTL
                     EMLHSLRDRYDYIIIDTPPVGIVSDALVLMNHADINVFIVRENYSKKEYIKTINEIHE
                     QGKAKQLCILLNDAGTNQRYGYGYGYSYGYHGYGYYDEENRKKKFFRKPKKA"
BASE COUNT          732 a          930 c         1064 g          892 t
ORIGIN      
        1 caacggctac ctgctgatct gggcagacga ccagaacacc acctggatcg actaccacac
       61 gaatttcaat cttaccctca gcggcgatac gatcctgttg tccgattcgc tgggcgtact
      121 ggccgacagc atactctacc agcaacagac gcaggacctg tccgcttccc gttgcgcgga
      181 tggaaccgga ccctttgtcg atggtaccac accgactccg cgtgcaatga acgattgcgc
      241 ctctaccggc tattacgtat caacggcatc ggacgcgttg atcgtctatc ccaatcctgc
      301 cagcgatctc gtttcgatcc gttccgagaa gtccatgcaa cgcgtggaga tcctggacct
      361 gcagggccag gtgatcgagc agcgggagct ttccgacgac aagcaagtgc agatcggact
      421 ggagcgcttc agtccgggat tgtatctgct gcgcgtcgac ggattgacac cggttcggct
      481 ggtaattcag taattcctta aaccgttcag actggccggg cgagcgatcg cccggccatt
      541 tttatcatgt accggaatgt gatttcgtat tgcaagcccg aagcaggcca gggaattttg
      601 cggggcaggt cagggagcct gcacagtgaa catcatccat gaacgacatt accgatcggc
      661 aggacgttat cacgctcgtc gattcctttt accggcaggt tcgcgagcac ccattactcg
      721 gaccgatctt caacgaggtg gcgaaggtcg attggacggc gcaccttccg aagatgtatt
      781 ccttttgggc tacgctgctc ctgggggaaa attcctattc cggcaatccg atgctgccgc
      841 accttgatct cagccgaaag gctacactgg acgaggaagc attcaacgcg tggaagcagc
      901 ttttcaatca aacggtcgat agcctattcg ccggtactaa agccgaagaa gcgaaggagc
      961 gcgccggcaa catcgcgcgt ttgatgttgc acaaggtgca atcggccaaa gcgtattgaa
     1021 agagtaataa tttaagcgaa aaaaaagggg cggtgatcat gtccgcccct tttcttgtat
     1081 tcatgccttc ttcggtttcc tgaaaaattt cttcttccga ttttcttcgt cataatagcc
     1141 gtagccgtgg taaccatagc tatagccgta gccgtacccg tagcgttggt tggtgccggc
     1201 atcgttgagc aggatgcaga gttgctttgc ttttccttgt tcgtggatct cgttgatcgt
     1261 tttgatgtac tccttcttcg agtaattctc gcgaacgatg aagacgttga tgtcggcatg
     1321 gttcatcagc accaaggcgt cgctcacgat ccccaccggc ggtgtgtcga tgatgatgta
     1381 atcgtagcgg tcgcgtaacg agtggagcat ttccagtgtc cgtttgttgg ccaggagttc
     1441 cgccggattc ggcgggaccg gaccggacgg gatgatctcc aatccgtcga ccgaagtctt
     1501 gtggatgact tcttccagcg tcgcgcgacc gatgaggtag ttgctcaccc cgatggagtt
     1561 gtcgatgccg aaatcctgca ccagttgcgg cttgcgcagg tccataccca cgatgatgac
     1621 gcggtaatgc tgcaaggcca ggaccgccgc aaggttcagg gtcgaaaagg attttccttc
     1681 accgcccacg gagctggtga tgagtacgat cttgtgctga tcggccagtc cgaggaacat
     1741 caggttggcg cggatggaac ggaaggcttc ggcgatggcc gatttcggct tggcgctcac
     1801 caccaggcgt tcactgctgg tgtggtgtcc cacgacgccg atcacgggta ctttcgtctg
     1861 cttctccaca tcctcgcggt tgctgacagt gttcttcaga tagccctgca gggcgatgaa
     1921 gagaatgggc aataccagcg cgaggaggag cgtgatgatc gccaccgcct tgcggttcgg
     1981 gatcaccggg atctcgttga aggatgcgct gtccagcacc ttgttgtcgg acacggccgt
     2041 tgccttggcg ataccggtct ccgctttctt ctgcaggagg tagaggtaga tattctcatt
     2101 caccgagaaa ttgcgctgga tgccgagcag ttcccgttcg atgttcggga tcttgcggat
     2161 cgagccttcg tattccgcca actggccctg gatcgtaccc aggctgacgc gcgtctggtt
     2221 gcggaggctg ttgatgtttt cgatcaacga cgctttggtt tcttcgatct gctggtcaat
     2281 gactttgatc gccggagagt tctcactggc gccgtaccgc aggctgcgcc gtttggtctg
     2341 cagattcttc agttgaccga tgaggtcgat cagcagggga tccggtgtgc ccaggctgga
     2401 aggagccagt tggtcgaggt ccttgttcga ctttacgtag ttgtacaggt aatccagcga
     2461 cttcaattcc agctcggctt tggcccggtc ggcatcgacc cgtgtgacac gctcgaggta
     2521 ggccttggat tcttcgctca ggtcaacggt tcctttttgt tccttgaacc gttggagttc
     2581 cagttcggtc gcgttgaggg tcttggagat ctcctcgagt tgctcgtcca cgaacttcag
     2641 cgtcagcgcg gcgacggaag acttgtcctt aacgtcctgg ttgatgtaga cttttcccag
     2701 ggtgttgagg aaatccacgg cacgttcggg cacctggtcc tggatggtga tgttgatgat
     2761 ggtcgcgtcc ttgttgatcg gttcggcttt catcttttcc gaataccagg caatcagttt
     2821 gttggtcgat tggatgacaa agcggaagtc atcgtcctga cgtgccgcga aggaaggatc
     2881 gttccttaca atgcggagtt tgaatcgggc tgtctggacc tcttcgccga agcggtgttt
     2941 ggaggagtag ttgaaatcgt ccaccaaccg gttgtccgcc tcgatctcga gctgataggt
     3001 cagtgaatcc agcaggcgga tgtgaaactc ttcatcgtac aagccgtctt gaacatacag
     3061 cggttcaacg atgaaggggt tgttcttgta aatcggataa cggaaaaaag tggtctttcc
     3121 gtaataactg acgcccaatc cgagctcttc gatggtttcc tgcaggacgg atcgggatcg
     3181 cagaatgccg atctccgtgg cgacgttttt gacgttgccg aacaggtcgc cggtcaggat
     3241 gtcttcgata ttgctcgact tgttcttgct atccttgatg agcacactcg tattcgcttc
     3301 gaataccggt agtgtaaaac ggatgtacaa caggccggcg gttccgaaaa cgagtaagga
     3361 gatcaggaag tagtgccagt tcctgcggat gttcgaaaag agcaggcgca ggtcgatctc
     3421 gtcgtcgttg cgtggacggt tgtcggtgct ggcgcgcatg ttatttggtt tgggtcagga
     3481 aaaaggtgac ggcaatgatg gcggtggaga tcaccgaggc gaagagcgtg accgaagggt
     3541 ttatgttctg gaaagcgcgg cggcgtaccg gagcgacgta cacgacatcg tcaggatgca
     3601 ggtaccaggt ttcgggtg
//
LOCUS       DAOTON010000197         3615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20418_length_3615_cov_3.743258, whole genome shotgun sequence.
ACCESSION   DAOTON010000197 DAOTON010000000
VERSION     DAOTON010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20418_length_3615_cov_3.743258"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="P5292_05030"
     CDS             complement(<1..320)
                     /locus_tag="P5292_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="UbiA prenyltransferase family catalyzes the
                     transfer of a prenyl group to various acceptors with
                     hydrophobic ring structures in the biosynthesis of
                     respiratory quinones, hemes, chlorophylls, vitamin E, and
                     shikonin; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HRS38526.1"
                     /translation="MFHFLRLFRPLNLLLIALTQFLVRYCLILPAFQTEKNVTGEFPA
                     HLDKWTFCLLVFSTVLIAAGGNVINDVYDRYIDEKNRPGTNPIGSRISPELARKLFMT
                     LSVTG"
     gene            complement(325..831)
                     /locus_tag="P5292_05035"
     CDS             complement(325..831)
                     /locus_tag="P5292_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRS38527.1"
                     /translation="MNFKELLSGVKAFVFDVDGVMTDGMLVVLQDELHRMMNIRDGYA
                     LHAAIHAGYPICVISGGKSESVRTRLNNLGITEVHLGVKDKPSILNEFLKAHDLNATE
                     VLYMGDDLPDLDVMRIVGVRCCPADAAPEIRAISLYISPLAGGRGCVRDVIEQVMRLH
                     GNWPGLNA"
     gene            complement(858..1622)
                     /locus_tag="P5292_05040"
     CDS             complement(858..1622)
                     /locus_tag="P5292_05040"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRS38528.1"
                     /translation="MNIVLIGAGNVGQQLALALKKAGHRIVQVHGKGMHRARSLARKL
                     GSNHLKDIRELDTSADIILLAVPDDAIPAILKSIPATKQLIVHTAGNVPLSVFGRKFP
                     NAGVFYPLQSFSADRPLRMRHIPILIEARTPQLRRKLNLLGKSISEEVVAVSSEQRQW
                     LHLAAVISNNFTNHLLYLSSSLLQRHRLPFRLLYPLIRETTEKAIALHPLAAQTGPAR
                     RGDRKTMQKHLSLLASDPRLRSLYRLMSNGIAAVKR"
     gene            complement(1624..>3615)
                     /gene="ccsA"
                     /locus_tag="P5292_05045"
     CDS             complement(1624..>3615)
                     /gene="ccsA"
                     /locus_tag="P5292_05045"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRS38529.1"
                     /translation="SMILGVIVFGYKLGSNPFTLLREHPDFAQLPFIRFPDYLQRLKD
                     GRGLNPLLQNYWMTIHPPTLFLGFATTIVPFAYAIGGLLRKQYDQWIRPALPWTFFSI
                     MILGTGILMGAAWAYESLSFGGFWAWDPVENASLVPWMTLTGLGHVMLIYKHRGRALW
                     SNYILAVVTFFFILYSTFLTRSGILGNSSVHAFTDLGMSGQLLVYMLFFVVLAGFLLF
                     RNRKGIPLGPEEEHFSSREFWMFIGMLVLVIGSLQITFTTSTPVINKLFGTKLAPPAD
                     PIDHYNRWQVPVAILLSLLIAVAQYFRYKQSDWKTWLNKISLSLLTASILTVLLVLAL
                     GSFRYHYYALLFASLFAVTANLDYFIRVLKGKFRNAGASIAHIGMGLILCGALISNGK
                     KQVISQNLKNIDLGKDFPNRENIMLEQGLDTLPMGPYYVTYTGKEQEGIHVYYKIGYF
                     TQDETGQKQQAFELRPFVQLNERMGNVAEPATKHFLTRDIYTHITYAELEKKGSGDTS
                     DYQAPKSHMMAIGDTVFTSNSFVILEALDKQPASETEHLHEHDIAVGAKLIVQDINKR
                     TYTPEPIFLIRDMSMYTKPASVDELGLQFDFNKIDPNTGKIELLIREKKSNKRDFVIM
                     KAIIFPGINILWIGCVMMILGTVLAVRHRIRVNQSSESQ"
BASE COUNT          908 a          896 c          988 g          823 t
ORIGIN      
        1 ccggttaccg aaagtgtcat aaacagtttg cgggcaagtt ccggactgat cctggaccct
       61 atcggattcg tacccggccg gttcttttcg tcaatgtatc ggtcgtagac gtcattgatc
      121 acattgccgc ccgctgcaat caagaccgtc gagaagacga gaaggcagaa cgtccacttg
      181 tctaagtgcg cagggaattc acccgttaca ttcttttccg tctggaaggc cggaagaatc
      241 agacaataac ggaccagaaa ctgcgtcaag gcgatgagca gcaggttgag gggtcggaag
      301 agccggagga aatgaaacat gggttcaggc gttcagtccg ggccagtttc cgtgcaaacg
      361 catgacttgt tcgatcacat cacgcacaca accgcgtcct ccggccaagg gcgaaatgta
      421 caagctgatt gcccgaatct cgggtgcggc atccgcagga caacatcgta cgcccacaat
      481 ccgcatcacg tcaagatccg gaaggtcgtc gcccatatac aacacttcgg ttgcattcaa
      541 atcgtgcgct ttcaggaatt cattaagaat ggatggtttg tccttcacgc caaggtgcac
      601 ctcggtgatg ccaagattgt tcagacgggt acgaacgctc tcggattttc ctccggagat
      661 cacgcagatc ggataaccgg catggatggc cgcatgcaaa gcataaccgt cccggatatt
      721 catcatgcga tgcaattcgt cctggagaac gaccagcatt ccgtcggtca tgaccccatc
      781 cacatcgaac acgaatgctt ttactccgga caaaagttcc ttgaaattca tgcgaccgaa
      841 gatagcgatt tcgacattta cctttttaca gcggcgatgc cattgctcat caaacggtaa
      901 agtgacctga gccggggatc ggaagcgagc aagcttaaat gcttttgcat tgtcttacga
      961 tcacctctcc gtgcaggccc tgtttgagct gctaaagggt gcagtgcaat ggctttttct
     1021 gtcgtctcac gaatgagggg ataaagcaag cggaagggca gtcgatgtcg ttgcaggagg
     1081 gaggatgaca aataaagcag gtggttcgtg aaattattgg aaatgactgc agccaggtgt
     1141 aaccattgac gttgctcaga cgagacagcc accacttctt cggaaatgga tttccccaac
     1201 aggttcagtt ttcggcgtag ctgcggggtg cgcgcttcga tcaggatcgg aatgtgtcgc
     1261 attcgaagtg gccgatcggc ggaaaagctc tgaagcggat agaagacccc ggcattcgga
     1321 aattttcgcc cgaataccga gagcggaacg tttccggctg tatggacaat cagctgtttc
     1381 gtggcgggga ttgatttcaa tattgccgga atcgcgtcat ccggcacagc aaggagaatg
     1441 atatcggcag atgtgtcaag ttcccggata tccttgaggt gattcgatcc aagctttcgg
     1501 gcaagactcc tggcccggtg cattcctttt ccgtgtacct gaacgatcct atggccggct
     1561 ttctttaagg cgagagcgag ctgttgcccg acgtttccgg cgccgatcag aacgatgttc
     1621 atgttattgt gattcggaag attggttcac tcggatccgg tgtcgaacag ccagcacggt
     1681 tcccaggatc atcatcacac acccgatcca aaggatattg atacccggga aaatgatcgc
     1741 cttcatgatc acaaaatcgc gcttgttgct cttcttttcc cgtatgagca actcgatctt
     1801 acccgtgttg gggtcgatct tgttgaagtc gaactgcaac ccgagttcgt ccaccgaagc
     1861 aggtttcgtg tacatcgaca tgtcccggat caaaaagatc ggctccggcg tataggttcg
     1921 cttgttgatg tcctgcacaa tcagcttggc gccaaccgcg atgtcatgtt catgcaaatg
     1981 ctcggtctcg gaagcgggtt gcttatcgag tgcttccagg atgacgaacg aattgctggt
     2041 aaagaccgta tcgccgatcg ccatcatatg cgatttcgga gcctggtaat ccgaggtgtc
     2101 gcccgaacct tttttctcta attcggcata ggtgatgtgc gtgtaaatat cgcgcgtcag
     2161 gaaatgcttg gtggcgggtt ctgccacatt tcccatgcgc tcattcaact ggacgaacgg
     2221 gcgcaattcg aaagcctgtt gcttctgacc ggtttcatct tgtgtaaaat agccgatctt
     2281 ataataaacg tgaatgcctt cctgttcctt gccagtatag gtgacataat aaggccccat
     2341 cggtaaagtg tccaggccct gttccaacat gatgttctcg cggttgggaa aatccttacc
     2401 gaggtcgatg tttttcaggt tttgtgagat cacctgcttc tttccgttcg agatcaatgc
     2461 gccacagagg atcagaccca tgccgatgtg cgcgatggag gcgcctgcgt tccggaattt
     2521 ccccttcagc acccggatga aataatcgag gttggccgtc accgcgaaga gtgaagcgaa
     2581 cagaagggca taatagtggt aacggaagga tcccagcgcc aatacgagca ggaccgttag
     2641 gatggaagcc gtcaagaggg aaagactgat cttgttcaac caggttttcc agtcgctttg
     2701 cttgtagcgg aaatattggg cgaccgcaat cagcagactg agcaggatcg ccacaggtac
     2761 ttgccagcgg ttgtaatgat cgatcgggtc ggccggagga gccagcttgg ttccgaacaa
     2821 tttattgatg accggtgtcg aggtggtaaa ggtgatctga agggaaccga tgacgagtac
     2881 cagcatgccg atgaacatcc agaattcccg tgaagagaaa tgctcctctt ccggtcccaa
     2941 gggtattcct ttgcggtttc ggaataagag aaagccagcc agtacaacga agaacaacat
     3001 gtacaccaat agctgccccg acattccgag atcggtaaag gcgtgtacgg aagaattccc
     3061 caggataccg gaacgtgtca gaaaggtaga gtagaggata aagaagaatg taacgaccgc
     3121 caggatgtag ttcgaccata aggcacgacc gcgatgcttg tagatcaaca tcacgtgccc
     3181 caggccggtg agcgtcatcc aggggaccag ggaggcgttt tcaaccggat cccaagccca
     3241 gaagccgccg aaactaagcg actcataagc ccacgcagca cccatcagga taccggtacc
     3301 caggatcatg atgctgaaaa aggtccacgg cagtgcagga cggatccatt gatcgtattg
     3361 tttgcgcagg aggccaccga tggcataggc gaacgggacg atggtggtcg caaaaccgag
     3421 gaaaagggta ggaggatgaa tcgtcatcca gtaattctgg agcagcgggt tcaagccgcg
     3481 gccgtctttt agccgttgca ggtaatcggg aaaacgaatg aaaggcaatt gggcgaaatc
     3541 cgggtgctct cttagcaagg tgaaaggatt actgccgagc ttatagccaa atacaataac
     3601 ccccagtatc attga
//
LOCUS       DAOTON010000198         3613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20431_length_3613_cov_3.784992, whole genome shotgun sequence.
ACCESSION   DAOTON010000198 DAOTON010000000
VERSION     DAOTON010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20431_length_3613_cov_3.784992"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1507)
                     /locus_tag="P5292_05050"
                     /pseudo
     CDS             complement(1..1507)
                     /locus_tag="P5292_05050"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
     gene            1722..2258
                     /locus_tag="P5292_05055"
     CDS             1722..2258
                     /locus_tag="P5292_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38530.1"
                     /translation="MARLKKSKSILRLVFITALLLSSTVHGRAQGGYKTGLGFRLSGV
                     TSGISIKHFTGGTGAIEGIIGFRYHSWIITGLYEKHQAIPNAEGLSWFYGGGAHIGFY
                     DEGYRYYYYKVRGNKYVIVDEDRNERMALDADFILGMEYKFKNAPITIGLDAKPFIDI
                     VPDFSGYWDAALSFRFTL"
     gene            2262..2747
                     /locus_tag="P5292_05060"
     CDS             2262..2747
                     /locus_tag="P5292_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRS38531.1"
                     /translation="MLIRPATLQDQPGILEIYNESVLHSTATFDTDPRSPEKQLEWFN
                     RHKANHPVLVAEDDGKIAGWASLSPWSDRCAYDSTVEVSVYLAPEQRGKGLGFQLLQQ
                     VTEAGRKAGNHTVISRISSDNLASIRIHEKAGYSTVGTMKEVGFKFGRFLDVVMMQIV
                     F"
     gene            complement(2786..>3613)
                     /locus_tag="P5292_05065"
     CDS             complement(2786..>3613)
                     /locus_tag="P5292_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38532.1"
                     /translation="YVTTPGGSPVFHEGVARFDGANWTVWCDTNSALPDRPVEHLFID
                     RNDAVWMVSLGLWRYDGTSWTQPPQPPGLNGNSRLAEDSSGGIWVSLNDFGLARFDGT
                     SWTTYPNPLGFGTGTYQDLAVAADQSVWAASYSHVARFDGSNWQLFDAANSPLDVNCT
                     AVEADKYGNVWFGYPVGLFAYRDGGVVLDAPEVPAERPFRIHYHAGSKHLSLTWDDDY
                     LPVSAQVISPEGKTITIACPRNDQGFRLDFSRLAAGSYVLRLLDRNGLPRIARFVVL"
BASE COUNT          827 a         1050 c          925 g          811 t
ORIGIN      
        1 tcttgtagcc ggtcgcctgt ccgggccaaa cgatgtaccg gtcgatctcc ttgcggcaat
       61 cgccttcggg attcggcgtg tttttcaaaa agtactcaaa cgcttgtgtg cgcgtccatt
      121 tcttgtagtg aatgccgaca tctactacga gccgagcagc gcggaagacc tcgttggaca
      181 aacggccgaa attggagtac gggtctttgt agaaaccgat ctccttcggc accaactccg
      241 agtacagcgc ccaaccttcc acgtacgcga cattgccgcc gtggcgacgg aacttcggaa
      301 cacctttcag ctcctgcgcg atggcgatct gcatatggtg acccggtatg ccttcatgat
      361 acgccaacgc ctccgcctgg tagatcggct ggtccttcat gttgtacagg ttgatgtagt
      421 aggtgccagg acgtttgccg tcctccgaag gatcttcgta aaacgcgcca cccgccgact
      481 gctcgcggaa tggttccacc ggcttgacga caatcgtcgc cttcggtttg gtaacgaaca
      541 attcgtcgag gtgctgccgc atggtatcga tgatgccggt agccaacaag cggtaatttt
      601 cttttccctc ccgcgtgtcg ggaaaataga acttgggatc atcccgcagg taggaaaaga
      661 aagcgctgat attgtggtcg cgccatttca cctgttccat gatcttgcgc atctcgtcgt
      721 gaatgcgctt cacttcccgg aggcccgtat cgaatacctg ctccggggtc atgttcgtag
      781 tcgtggtctt ctcaagcgca tgacgataga actcattccc cttcgggaac ttccacgcgc
      841 cgtcgtcctc tttagcccgc ttctcctgct ccttcagata ggccatgagt cgttcctacg
      901 ccggcttcac gacttccacc aatgcacggg aagcttcacc ctgcaatcgg gcgatcttcg
      961 aaccgtcgat acctttcagc gctgacagct ttttcgagaa gtcctcatac aacggacatt
     1021 taccgctgcc gctgaacgga tatccgctga tcaagttgtt acagtcgttc aggaccatcg
     1081 gatagacaaa tcgcggagga atgatcccaa ggctgtcacg tacccgcaat ccatccagga
     1141 cctgatccat caacggtccg attcccttca accgttgcac atacgcttcc gcgtccccga
     1201 tggaatcgat ccggtgcatg ttgatcagga atgccggaat acccgattga acgccgtcca
     1261 tctggttcac cggataattg tgatagcgcc acggaaacgc gtcgaacagt tcctgcgcgt
     1321 cgtgttcgaa caagcggtaa ctgagtcgcg cctgatcgtc cagcttgtcg aattcaaccg
     1381 tcttcttcaa ctcctccaac tgccggcggg taagttcatt tcgcttcacc tgaaacgaat
     1441 ccgaacggtc ggtccactga ccgtaatcct gcttgatccc gaggtaggtt tgcagttcgg
     1501 gatccatatc gaccatctcg tcgaagcaac gatcgaagaa ggcattcgcc ttggcggact
     1561 ccgcggtaac ttcctccggt gtgaattttt taacagatga cttcgaatcc tgacggcagg
     1621 atgaagcgaa taaaatcagt aatagaaacg ttaaacgaag gggattagct gctgccaatg
     1681 ctttattttt gaatgtcgaa caaagataag ggacgaacgc catggccaga ctcaaaaaat
     1741 cgaaaagtat cctccgactg gttttcataa ccgccctcct gctctcctca acagttcatg
     1801 ggcgcgcgca aggcggctat aagaccggac tcggctttcg cctttccggt gtcacctccg
     1861 gcatttccat caagcatttt accggcggta ccggcgcgat cgaagggatc atcggctttc
     1921 gttatcacag ttggatcatc accgggctgt acgaaaagca ccaggccatt ccgaatgcgg
     1981 aaggcttgtc ttggttctac ggcggaggcg cgcacatcgg attctacgac gaaggttacc
     2041 gttactatta ttacaaagtg cgtggcaata aatacgtaat cgtcgacgaa gatcgaaacg
     2101 aacgcatggc gctggacgcg gatttcattc tgggaatgga atacaagttc aaaaacgctc
     2161 ccatcacgat cgggctcgac gccaaaccgt tcatcgacat tgtaccggat ttttccggct
     2221 attgggacgc ggcactttcc ttccgcttta cgctttgatg catgctcatt cgaccggcaa
     2281 cgctgcagga tcagccaggc attctggaaa tttacaatga atccgtcctg cattccacgg
     2341 cgacattcga taccgatcca cgttcgccgg aaaaacaact ggagtggttc aaccgccaca
     2401 aggccaacca tccggtactg gtagcagaag acgatggcaa gatcgccgga tgggcgtcgc
     2461 taagtccctg gagcgatcgc tgcgcgtatg attcaaccgt tgaagtcagt gtctacctcg
     2521 cgccggagca gcgtggaaaa gggttgggat ttcagctctt gcagcaggta acggaagcag
     2581 gcaggaaagc cggcaaccac acggtcattt cccgcatctc gagcgacaac ctcgccagca
     2641 tccgcatcca tgaaaaagcg ggatacagta cggtcgggac catgaaggaa gtaggtttca
     2701 aattcggacg cttcctcgat gtggtgatga tgcagatcgt cttttaactc ctactcgcct
     2761 ttaccgctct tcaaccaggc tacattcaca atacgacaaa ccgcgcgatc cgtggcagac
     2821 cattccggtc gagtagtcgt agcacataac ttccggccgc cagtcgtgaa aaatcgagcc
     2881 ggaacccctg gtcgtttcgc ggacaagcaa tcgttatcgt tttcccttcc gggctgatca
     2941 cctgcgcgct gaccggcaga taatcatcat cccaggtcag ggaaaggtgc tttgaacccg
     3001 catgataatg gatcctgaac ggtcgttctg ccggcacttc cggcgcatcg agtactactc
     3061 caccatcgcg ataagcaaaa agcccgaccg gatacccgaa ccagacattg ccgtacttgt
     3121 ccgcctccac cgcggtgcaa ttcacatcca gcggactgtt ggcggcatcg aatagctgcc
     3181 aattcgaacc gtcgaaacgc gccacatgcg aatagctcgc ggcccagacc gactgatcag
     3241 cggcgaccgc aaggtcctga taggtcccgg ttccgaaacc gagcgggttc gggtaggttg
     3301 tccaggaggt tccgtcgaaa cgcgccagac cgaagtcatt caacgacacc cagatgccgc
     3361 cgctggaatc ctcagcgagt cgcgaattgc cattcagtcc cggaggctgc ggtggttgcg
     3421 tccagctcgt gccgtcgtaa cgccacagtc cgagtgatac catccagacc gcatcgttac
     3481 gatcgatgaa caaatgttca accggtcggt ccggcaatgc ggagttggta tcgcaccaga
     3541 ccgtccagtt tgctccatcg aatcgtgcga cgccctcatg aaacacagga ctaccacccg
     3601 gggtggtcac ata
//
LOCUS       DAOTON010000199         3596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20562_length_3596_cov_2.735385, whole genome shotgun sequence.
ACCESSION   DAOTON010000199 DAOTON010000000
VERSION     DAOTON010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20562_length_3596_cov_2.735385"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="P5292_05070"
     CDS             <1..794
                     /locus_tag="P5292_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRS38533.1"
                     /translation="GAVLTGEVKDVLLLDVTPLSLGIETMGGVMTKLIESNTTIPTKK
                     SEVFSTASDNQPSVEIHILQGERPMARDNRTIGRFHLDSIPPAPRGVPQIEVTFDIDA
                     NGILHVTAKDKATGKSQNIRIEASSGLTDDEIKKMKAEAEANADADRKVKEETEKVNQ
                     ADSLIFQTEKQLKEYGDKLSSGNRENIQRALSELKDAHKERDLGKIDTALANLNNAWQ
                     AASEEMYKASQQQGAPGADQAAGGQGKADGEVTDVDFEEVKDEKK"
     gene            905..2518
                     /locus_tag="P5292_05075"
     CDS             905..2518
                     /locus_tag="P5292_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38534.1"
                     /translation="MMARSGFHLIVFWLCLTGSLLAQPAAPFARYIGSPGNYPPEYRI
                     QSGPDGYFLLHNFVDSTLFVPQLSKVRNNGAAEWSRDIALSGVVSPRARLLLADTTGM
                     LMAGTLQQNGYKTFVTRLDTSGGLLSNLVIDFGGYNEPVVLRRGRASCLIAGYRDFPD
                     GLGRFTFDMTMLRLDSNDQVVRASASGNDSTDFTLTAAAILRDGRTISVGEVGNRAMA
                     RFQSQLAWWSDRDSLLRMLQVDDGSFYTRLRPVEIILSGDSALFVAASGSTIAGNEHE
                     VVVFKLDTAGQMIWSRRYYQIAYDLEVRTMATDRFGRICLTGNCYRTVNDNGTFLLRL
                     DQDGNVLDGALFKAPVFTFGGNATTDLLIEQDGSWTYPVYYYAGNLYTPALVHSDTSL
                     LSVCADLNEQFVFAMAGANLMLLHMTPPDQEFLSVAPDTLGISFTNRSVHEGDLCLAV
                     TIENDSYTNGAVIKVYPNPASDRVFLSGLSGDLELSILDVSGRAVYSTHQIRYEEGDP
                     IRLPELTPGLYLLRVRHEEGSYIHRLLIR"
     gene            complement(2528..3418)
                     /locus_tag="P5292_05080"
     CDS             complement(2528..3418)
                     /locus_tag="P5292_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRS38535.1"
                     /translation="MKRAYFQMHLAIFIWGFTGLLGKLIQLNEGMLVWYRILLSWISF
                     SLIVLPGKGWPKLNRSEWMNISGIGILVMLHWLTFYGSIKLSSISVAMICLSSIALFS
                     SFIEPLVNRQRFDHVEILFSAMALTGIFTIYRSDASASAGILVGLISALLSATFSVFN
                     RRIASRYDPTIISFIELGSGFSFLTLLLPVYVLVQPTHQFIPTLTDWIYLLILALVCT
                     VWTWILSLKALRKVSAYTMALALNLEPVYGIILAVLFANEGKILNSGFYFGAVIILLT
                     VVLHTIYRYRKTRRAETLQT"
BASE COUNT          886 a          906 c          980 g          824 t
ORIGIN      
        1 agggcgctgt actcaccggt gaagtgaaag acgttttgct cctggacgtg accccactgt
       61 cgctcggtat cgaaaccatg ggcggtgtga tgaccaaatt gatcgagtcg aatacgacca
      121 ttccgaccaa gaaaagcgaa gtcttctcaa cggcttccga taatcaaccg tctgtagaga
      181 tccacatcct gcagggcgag cgtccgatgg cccgcgacaa ccgcaccatc ggcaggttcc
      241 accttgacag cattcctccg gctccccgcg gtgttccgca gatcgaagtg accttcgata
      301 ttgatgccaa cggcatcctg catgtcaccg caaaagataa agccaccggc aaatcgcaga
      361 acatccggat tgaagcttct tccggtctca ccgacgatga gatcaagaag atgaaagcgg
      421 aagcggaagc taacgcggat gccgaccgta aagtgaagga agaaacggag aaagtcaatc
      481 aggccgactc gctgatcttc cagacagaaa agcagctcaa ggaatacggc gacaaactct
      541 cctctggtaa ccgcgaaaac atccagcgcg cactcagtga actgaaggat gcgcacaagg
      601 aacgcgacct cggtaagatc gacaccgctt tggccaacct caataacgca tggcaggctg
      661 cctctgaaga gatgtacaaa gccagtcagc agcaaggagc tcccggagct gaccaggcag
      721 ccggcggaca aggtaaagcc gatggtgaag tcaccgacgt tgattttgaa gaggtgaagg
      781 acgagaagaa gtaatcgacc gatccgattt ttccgaaatg gccgaccagg catacctggc
      841 cggccatttt tttatgctct tcgtcatgtc gatcaagtgg attcacagct acctttaatg
      901 ccttatgatg gcccgaagtg gtttccattt gatcgtattt tggctgtgtc tcaccggcag
      961 cttgttggct cagcccgctg ctcctttcgc gcgttacata ggatcgccgg gtaattaccc
     1021 tccggaatac cggatccaat ccggccctga cggctatttc ctgctgcaca atttcgtcga
     1081 ttccaccttg ttcgttccgc aactgtcaaa ggtccgaaac aatggtgctg ccgaatggag
     1141 ccgcgatatc gccctttcgg gtgtagtgtc gccgagggcc agattgctcc tggctgatac
     1201 gaccggtatg ctgatggccg gaactcttca gcaaaatggt tacaagacat ttgttacccg
     1261 gttggatact tccggtggtc tgctgtcgaa tctggttata gacttcggtg gttacaacga
     1321 accggtcgtt cttcggagag gtagggcaag ttgtctgata gccggatatc gggattttcc
     1381 ggatggactt ggtcgcttca cctttgatat gactatgctg cggttggatt cgaatgatca
     1441 ggtagtacgt gcttcggcca gtggaaacga ttcaacggat tttaccctga ctgcagcagc
     1501 catcttgcgt gatggtcgta caatctcggt cggcgaggtg gggaaccggg caatggcacg
     1561 cttccaaagt caactggcat ggtggtcgga tcgagacagc ctgcttcgga tgttgcaggt
     1621 ggatgacggg agtttttaca cacgcttgcg tccagtggaa atcatcctct ccggtgattc
     1681 cgccctcttc gttgctgctt ccggaagtac gattgccgga aacgaacacg aagtggtggt
     1741 gtttaaattg gatactgccg gccagatgat ctggtcgaga cgttactatc agattgccta
     1801 tgacctggag gtccgaacca tggcgaccga tcggttcggg cggatttgtc tcaccggcaa
     1861 ctgttaccgt acggtaaatg acaacgggac attcctctta cggctggatc aggatggcaa
     1921 tgtgttggat ggggccttgt tcaaggcgcc ggtcttcacc ttcggcggca acgcgacaac
     1981 cgaccttttg atcgagcagg atggcagctg gacgtacccg gtctattatt atgccgggaa
     2041 tctctatacc cccgcattgg tgcattccga taccagctta ctgagcgtat gcgctgatct
     2101 gaatgaacaa ttcgtttttg cgatggccgg ggcgaatctg atgttgcttc acatgacacc
     2161 gccggatcag gaatttttgt cggttgcgcc cgatacactc ggcatcagtt ttacaaaccg
     2221 aagcgttcac gagggtgatt tatgtctcgc tgtaacgatc gaaaacgatt cctatacgaa
     2281 tggcgcggtg atcaaggtgt atcctaatcc ggccagtgac cgtgtctttt tatcgggctt
     2341 atcgggtgat ctcgaactga gcatcctcga tgtatcgggt cgcgcagttt attcaaccca
     2401 tcagatccgt tatgaagagg gtgatccgat cagattaccg gagttgactc cgggtcttta
     2461 tctgctaagg gttcgccacg aggaaggcag ttacatccac cggttgttga tcaggtaagg
     2521 acctgcctca cgtctgcagc gtctccgccc ggcgtgtttt tctgtagcgg tagatcgtgt
     2581 gcaatacgac cgtcagcaga atgatgacag cgccgaaata aaatccgctg ttgagaatct
     2641 taccttcatt cgcgaagagt acggccagaa tgataccata gaccggttcc aggttaagcg
     2701 cgagcgccat ggtataggcc gacacttttc gaagcgcttt gagcgaaagg atccaggtcc
     2761 agacggtgca caccaaggcc aatatcaaga ggtaaatcca gtccgtcagc gtcggaatga
     2821 actgatgcgt tggttgtacc aatacgtata ccggtaatag cagcgtcaga aagctgaaac
     2881 cggatcccaa ttcaatgaag ctgatgatcg tagggtcata acgtgacgcg atgcgccggt
     2941 taaagacact aaaagttgcg ctgagcagag cgctgatcag gccgaccagg atacctgccg
     3001 aagcggatgc gtcggaccgg tagatcgtaa agatcccggt cagggccatg gcggaaaaca
     3061 ggatctcgac atgatcgaat cgctgccggt tgacaagtgg ttcaataaac gacgagaaca
     3121 gcgcgatgga agaaaggcag atcatcgcta ccgaaatact ggaaagcttg atcgagccgt
     3181 aaaaggtcag ccaatgcaac atcaccagga tgccgatacc cgaaatgttc atccattcgg
     3241 accgattcag tttcggccat ccttttcccg gtaggacaat gagcgaaaag ctgatccagc
     3301 tcaggaggat ccggtaccac accagcatgc cttcattcaa ctggatcaat tttccgagca
     3361 ggcccgtaaa cccccatata aagatcgcca ggtgcatctg gaagtaagca cgcttcatag
     3421 ggaggatgat gctggtagtt taacggttta agtgttgtgg tcaggggaga aatcggtcaa
     3481 gctcccggta tagttcaaag atcggcaaac ccatcacgtt aaagtaactt ccatcgatcg
     3541 atgaaatgat cggtacatgt ttgtcgggat cggcagcaag cgattcttca aagagc
//
LOCUS       DAOTON010000200         3589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20609_length_3589_cov_2.465761, whole genome shotgun sequence.
ACCESSION   DAOTON010000200 DAOTON010000000
VERSION     DAOTON010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20609_length_3589_cov_2.465761"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="P5292_05085"
     CDS             complement(<1..129)
                     /locus_tag="P5292_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRS38536.1"
                     /translation="MYWTLELAQHLEDAPWPATKDELIDYAIRSGAPLEVIENLQEL"
     gene            complement(261..809)
                     /locus_tag="P5292_05090"
     CDS             complement(261..809)
                     /locus_tag="P5292_05090"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019949704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRS38537.1"
                     /translation="MKIYTRKGDQGETSLIGGTRVPKHHLRIEAYGTIDELNSWTGLL
                     RDHITDPHIREELAVVQDRLFTIGSQLAADPDASRMQLPDILSSDILALESSMDRMDA
                     NLPEMRNFVLPGGHPVVSYCHVTRCVCRRAERLCVHLGENTPVKPEILQYLNRLSDYY
                     FVLSRQLAKDLGAQELPWKPRG"
     gene            complement(806..1528)
                     /locus_tag="P5292_05095"
     CDS             complement(806..1528)
                     /locus_tag="P5292_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS38538.1"
                     /translation="MALIDIRDISRRYTIGSETVNALRSVTLQIEKGEYVALMGPSGS
                     GKSTLMNILGCLDTPSGGTYTLNGKEVSRMTEDELAEIRNKEIGFIFQTFNLIPRSNA
                     LDNVALPLVYAGINKDERDEKAKKALDDVGLTDRMLHKPNELSGGQRQRVAVARALVN
                     DPSIILADEPTGNLDSKTSSEIMSLFEEIHTRGNTIIVVTHEEDIARYAHRIVRIKDG
                     MIESDARNPNITRADRPLTENA"
     gene            complement(1589..2377)
                     /locus_tag="P5292_05100"
     CDS             complement(1589..2377)
                     /locus_tag="P5292_05100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS38539.1"
                     /translation="MNPLYPALEYCRYRVRASNEHGLHSPFVYELYNSVIRDETPYYV
                     FGLIESIRARMILSDEVIDVRDFGTGGGRTGSQRRLGVSAIARRFVQSRRHGQLLFRL
                     VNRFRPGKVLELGTSIGITTLYLAAPSGNTEVWTLEGCPETARIAQENFDSVQAPNIH
                     PVVGEFSTTLPDVLRQMGTVDFAFLDGNHRYEATLAYFRQLLPFTHEHSVLVFDDIHW
                     SSEMRSAWEVIRQDPAVTISIDLYQFGIVFFRQGQARQHFTLQY"
     gene            2481..>3589
                     /gene="eno"
                     /locus_tag="P5292_05105"
     CDS             2481..>3589
                     /gene="eno"
                     /locus_tag="P5292_05105"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRS38540.1"
                     /translation="MSTIAEIRARQILDSRGNPTVEVDVVTENGVVGRAAVPSGASTG
                     AHEAVELRDGDKKKYLGKGVLRAVHNVNKTLNEELHGFPVYEQRLIDLAMIKIDGTEN
                     KANLGANAILGVSLACAKAAAIESGQPLYRYIGGVNANVLPLPMMNILNGGAHADNGI
                     DFQEFMIMPVGAASFSESLRMGTEVFHHLKAVLKKKGLSTNVGDEGGFAPNLKSNEEA
                     IQAVMDAIGEAGYVAGKDIFIAMDAASTEFFDTASGQYIFKKSTGDKLTPDQMAEFWQ
                     SWIAKYPIVSIEDGLAEDDWAGWKKLTELAGKKVQLVGDDLFVTNVKRLGDGIRQGVA
                     NSILVKVNQIGTLTETIEAVDLANRNGYSSVMSHRS"
BASE COUNT          810 a          936 c         1045 g          798 t
ORIGIN      
        1 gagttcctga aggttctcga tcacttccag cggggcaccg gagcggatgg cgtagtcaat
       61 cagctcatcc ttggttgcag gccagggcgc gtcttccaaa tgctgcgcca gttcaagggt
      121 ccagtacata atagtcgccg ggttaaggag taatcctggt tttttaaaag tccgcaaaaa
      181 taaaaatatt ccgttcagat gcacccgatg catcactttt tagaactgct tgaaaaacaa
      241 ggcgggatgg gggattgggc tcagccccgg ggtttccagg gcaattcctg agcgccaagg
      301 tctttggcca actgccggga gagtacgaaa tagtaatccg acaatcggtt caggtattgg
      361 aggatttccg gcttaacggg agtgttttcg ccaaggtgca cacacaggcg ttccgctctg
      421 cggcacacac agcgcgttac atgacaatac gaaacgaccg gatggccgcc cggcaacacg
      481 aagttgcgca tctcgggaag gttcgcatcc atgcggtcca tggatgattc caatgcaaga
      541 atgtccgatg acaggatatc cggcagttgc atcctggatg catcggggtc cgctgccagt
      601 tgagagccga tggtgaagag ccggtcctgg acgactgcta attcctcgcg gatgtgcggg
      661 tcggttatgt gatcccgcaa aagacccgtc caggagttca gttcgtcgat cgtaccgtac
      721 gcttcaatgc ggaggtggtg cttcggtaca cgcgttccgc ctatgaggga ggtttcgccc
      781 tggtcgcctt tccgggtgta gattttcatg cgttttcagt aagcggccgg tcggcccggg
      841 tgatgttcgg attacgcgcg tccgactcga tcatgccgtc cttgatgcgt acgatccggt
      901 gagcgtaccg ggcaatgtcc tcttcgtgcg taacgacgat gatcgtatta ccgcgtgtgt
      961 ggatctcttc gaataaggac atgatctccg aagaggtctt ggagtccaga ttaccggtcg
     1021 gttcatcggc caggatgatg gacggatcat tgaccagtgc ccgggctacg gcgacccgtt
     1081 gtcgctgacc gccggagagt tcgttgggtt tatgcaacat ccggtccgtc aaaccgacat
     1141 cgtccagtgc cttcttcgct ttttcgtcgc gttcatcttt gttgatgccc gcatagacca
     1201 acggaagcgc gacattgtcg agcgcgttgc tcctgggaat gaggttaaag gtctggaaga
     1261 tgaatccgat ctccttgttc cggatctcgg ccaattcgtc ttccgtcatc cggctgacct
     1321 ctttgccatt caacgtgtac gtgccgccac tgggtgtatc gagacatccg aggatattca
     1381 tcagggtcga cttcccggat ccggaaggac ccatgagcgc gacgtactcg cccttttcga
     1441 tctgaagggt gaccgaacga agcgcattta ccgtttcgct tcctatggtg tagcgtcggg
     1501 agatatcgcg aatgtcgatc agcgccatgg tggattcgtt tgcagcaaaa gtagccctta
     1561 atgtgttatc aaaacgttag ggatcgcttc agtattgaag ggtgaaatgt tgtcgtgcct
     1621 gaccttgccg aaagaatacg attccaaatt ggtagaggtc gatgctgatg gtgaccgccg
     1681 gatcttgtcg gatcacctcc caggcgctgc gcatctccga actccaatgg atgtcgtcga
     1741 atacgagtac ggaatgctca tgcgtaaagg gtagcagttg ccggaagtag gcaagggtcg
     1801 cttcgtaacg gtggttgcca tccagaaagg cgaagtcgac cgttcccatt tgccggagca
     1861 cgtccggaag cgttgtgctg aattctccaa cgaccgggtg gatgttcggc gcctgcaccg
     1921 agtcgaaatt ctcctgcgcg atgcgggctg tctccggaca gccttccagc gtccacactt
     1981 cggtgtttcc ggagggcgcg gcgaggtaaa gcgtggtgat cccaatcgag gtacccaact
     2041 ccagcacctt gcccggccga aagcggttga cgaggcggaa cagcagttgt ccgtgacgac
     2101 gagactgcac aaaccttcgg gcaatcgctg aaacacccag tcggcgctgg ctaccggttc
     2161 gaccgcctcc ggtcccgaaa tcacgcacgt cgatcacttc atcgctcagg atcatgcggg
     2221 cgcgtatgga ttcgatcagg ccgaagacgt agtaaggtgt ttcatcccga atcaccgagt
     2281 tgtataactc gtagacaaaa ggcgagtgca aaccatgttc gttcgaggca cgaacccggt
     2341 agcggcaata ttcgagtgct ggatagagcg gattcaacgg gacgaaggta aaaaaccggc
     2401 ttgggaggaa ggcgtcagga ttagcacaag tggccaaatg cccggttttt tccgtatttt
     2461 tgcgtcgcaa aaccaccatt atgagcacga tcgctgaaat tcgcgccaga cagatcctgg
     2521 actcccgcgg caatcccacg gtagaagttg acgttgttac tgaaaatggc gttgttggca
     2581 gggctgccgt tccttccggc gcatcaaccg gcgcacatga agcggtagaa ctgcgcgatg
     2641 gtgataagaa aaagtacctc ggaaaagggg tgttgcgggc cgttcacaat gtgaacaaga
     2701 cgttgaacga agagcttcat ggctttccgg tctatgaaca acggctgatc gacctggcga
     2761 tgatcaagat cgacgggacc gagaataaag ccaacctggg tgccaatgcc atcctggggg
     2821 tttcactggc ctgtgcaaag gcagcggcga ttgaaagcgg acaacccttg tatcgctaca
     2881 tcggtggcgt gaacgccaac gttcttccct tgcccatgat gaatatcctg aacggcggtg
     2941 cccatgccga caacgggatc gacttccagg aattcatgat catgcccgtc ggagctgcat
     3001 cgttttccga atccctccgg atgggaaccg aagtgttcca tcacctgaaa gcggtcctga
     3061 agaagaaagg tctttccacc aacgtaggcg acgagggcgg attcgcgccg aacctgaaat
     3121 cgaatgaaga agcgattcag gcggtcatgg atgcgatcgg agaggccggt tatgtagctg
     3181 gtaaggacat tttcattgcc atggatgccg cttcgaccga gttctttgac acagcctccg
     3241 gacagtacat cttcaaaaaa tctacgggcg ataaactcac ccccgatcag atggcggagt
     3301 tctggcaaag ctggatcgcc aaatacccga tcgtatcgat cgaggacgga cttgcggaag
     3361 acgactgggc cggatggaag aagctcaccg aactggctgg caagaaggtg cagctcgtcg
     3421 gcgatgatct cttcgtcacc aacgtgaagc gcctgggcga tgggatccgc cagggtgtcg
     3481 ccaattccat tttggtaaag gtcaaccaga tcggaaccct caccgagacc atcgaagcgg
     3541 tagacctggc caatcggaac ggttattcct cggtcatgag ccatcgttc
//
LOCUS       DAOTON010000201         3580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20672_length_3580_cov_3.334752, whole genome shotgun sequence.
ACCESSION   DAOTON010000201 DAOTON010000000
VERSION     DAOTON010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20672_length_3580_cov_3.334752"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="P5292_05110"
     CDS             <1..221
                     /locus_tag="P5292_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38541.1"
                     /translation="PLADIVKIERLKYSRYHLNNPVFNLHLKNTIRFYTGGNDAVEIT
                     DKSGTPYRIGSQHAGALETAVRKAMSGI"
     assembly_gap    365..464
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    824..923
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <924..1136
                     /locus_tag="P5292_05115"
     CDS             <924..1136
                     /locus_tag="P5292_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38542.1"
                     /translation="FSIRLFVVLHPYDWDSGDFWGLGIPRIFEVTGLFFHFGFSLYST
                     PMTGIRVIFGVWGFCEYLKYQDYFFN"
     gene            1498..1929
                     /locus_tag="P5292_05120"
     CDS             1498..1929
                     /locus_tag="P5292_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRS38543.1"
                     /translation="MQKEEQLTYTFKYAALTGRIIKCAMAVHSALGNGFQEVIYQRAM
                     GIEMRLDEIHFEREKEMPIHYKGRRIGTRRVDFLVEGIIAVEFKATTKFDNHHINQAM
                     NYLEAYNLEVGLLINFGERSLNFRRLTNKKHKPTASQSSNH"
     gene            2066..2683
                     /locus_tag="P5292_05125"
     CDS             2066..2683
                     /locus_tag="P5292_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRS38544.1"
                     /translation="MNSYLIRSLFLSLSLLLGLSSSAQVAVNPQVGMTLQALRDLGDG
                     IDVDRRAGFFGGIDFRIGNRVYFQPGLFFMQGGTLTKPDTVVSGGDLLVYRNAAKVKT
                     LIGADLIRKPLFRLRLNAGVSFDYHFSYTVKEGDTDLSIYRDNSFNFETGLGADIYRF
                     TVEAGRSLGLSDAFDVKYYDRNLNELHPRYEYWYLTVGFRLGGGL"
     gene            2812..3387
                     /locus_tag="P5292_05130"
     CDS             2812..3387
                     /locus_tag="P5292_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRS38545.1"
                     /translation="MKKYLIWIILGLLVLWGVGGYNGLVSSREGVNKAWANVETQYQR
                     RSDLIPNLVATVKGAADFEKSTLEAVTQARANATSIKIDPNNLTPEKLQEFQQAQAQL
                     GGALGRLLAVAENYPQLRAVQNFTDLQAQLEGTENRINEARRQYNEAARDYNISRAKF
                     PRVILANLFGFKDRPYFEADKGAEKAPEVKF"
     gene            3387..>3580
                     /locus_tag="P5292_05135"
     CDS             3387..>3580
                     /locus_tag="P5292_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRS38546.1"
                     /translation="MASDGRFTDAEHRQILDAIGAAERLTSGEIRLFVEDVCGENVLD
                     RAAYIFHELKMDRTAERNGVL"
BASE COUNT          799 a          811 c          854 g          916 t
ORIGIN      
        1 tccccctggc agacatcgtc aaaatcgaac gcttgaaata cagccgttat catttgaaca
       61 atcctgtttt caatttgcac ttgaaaaaca ccatccgttt ttacacaggc ggcaacgacg
      121 cggtagagat caccgacaag agcggcaccc cctaccggat cggatcgcag catgcggggg
      181 cgttggagac ggcggtgagg aaggcgatga gcgggatctg attggctagg ttctcttttg
      241 tatcttttcg tcgtactcca cccatgtgac tgggattcgg gtgatttttg agggttgggg
      301 attctgcgaa tatttgaagt aacaagacta ttttttcaat taggcttttc gtcgtactcc
      361 acccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatttga ctgggattcg
      481 ggagattttt ggggtttggg attctgcgaa tatttgaagt atcaagacta ttgtttcaat
      541 tcggcttttc gtcgtactcc acccatttga ctgggattcg ggagattttt ggggtttggg
      601 attctgcgaa tatttgaagc aacaagacta ttttttcatt tcggcttttc gtcgtactcc
      661 acccatttgg ctgggattcg ggtgattttt ggggtttggg gattctgcga atatttgaag
      721 taacaagact attttttcaa ttcggctttt cgtcgtgctc cacccatgtg actgggattc
      781 gggtgatttt tggggtttgg ggattctgcg aatatttgaa gtannnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnttttcaa ttaggctttt cgtcgtactc cacccctatg
      961 actgggattc gggtgatttt tggggtttgg ggattccgcg aatatttgaa gtaacaggac
     1021 tattttttca tttcggcttt tcgttgtact ccacccctat gactgggatt cgggtgattt
     1081 ttggggtttg gggattctgc gaatatttga agtatcaaga ctattttttc aattaggctt
     1141 ttcgtcgtac tccaccccta tgactgggat tcgggtgatt cttggggttt ggggattccg
     1201 tgaatatttg aagcacaatc acgctgaggt aaccagcgtt ctgttttgaa ataccgaaat
     1261 aaattgtctc cggatcattc agtaagatat acttattgca ctaaccataa tacaggatat
     1321 atcttttatg ctggttgttt tgaaattcat ctgctttcaa tacatcagcc atctgtatat
     1381 cattcgcata ctcttcatga atcaattgat ctaagggcaa cattaaattt gcattcgaac
     1441 catgcaggca gtacgagcgc ttcagaaatc aagagtcgct ttacagtatc ctagttcatg
     1501 cagaaagagg aacaactaac ctacactttt aaatacgctg cccttaccgg gagaatcatc
     1561 aaatgcgcga tggctgtgca ttccgcattg gggaacggtt ttcaggaggt gatctatcaa
     1621 cgggccatgg gaattgaaat gcgtttggat gaaattcatt tcgaacgaga aaaggaaatg
     1681 cccatccact acaaggggcg taggattggg acaaggaggg tcgatttcct tgtcgaggga
     1741 ataattgccg tcgaattcaa ggcaactacc aagttcgaca atcatcacat caaccaggcg
     1801 atgaattatc tcgaagcata caacctggaa gtcggattac tcattaactt cggagagcga
     1861 agtctgaact tccggagact tactaataaa aagcacaaac ccactgcttc acaatcctcc
     1921 aaccactgaa taagcctctg aaacttcatt ttactcacta tttgggcatc actaaaaatt
     1981 gaatttgact tccttcctgc tttactaata tttggacaat attgagttca gaaaacagtt
     2041 aaactagtac atctatgcca ctgtcatgaa tagctactta atccgctctc tttttctttc
     2101 attgtcgctg ctactggggc tttcctcctc cgcccaggtt gccgtaaacc cgcaggtggg
     2161 catgactttg caggctctgc gtgatttggg ggatggcatc gatgttgatc gccgtgccgg
     2221 gttctttggc ggtatcgatt tccggatcgg gaacagggtg tatttccagc cgggtttgtt
     2281 cttcatgcaa ggcgggacgc tgaccaagcc ggatacggtc gtgagcggtg gcgacctgct
     2341 tgtttacagg aacgcagcga aggtaaagac gctgatcggg gccgacctga tccgcaaacc
     2401 cttgttccgg ctgcggctga acgcgggtgt ttctttcgac taccatttct cgtatacggt
     2461 caaggaaggc gacaccgacc tgagtatcta ccgcgacaac agcttcaact tcgaaaccgg
     2521 gctgggcgcc gacatttacc gtttcaccgt ggaggcgggc cgttccctgg gactctccga
     2581 cgcctttgac gtgaaatact acgaccggaa cctaaacgaa ttgcacccgc gctacgagta
     2641 ctggtacctg accgtcggct tccgactggg cggcggcttg tgatttccgc tttctcctgc
     2701 aggattaacg aatagttaac gtagtttgca ataactttgc aggctgttcg tcattcctcc
     2761 ttgttggagt cggacaaaat ttaaccgctc gaaacttcac acacctaaac catgaaaaag
     2821 tatcttattt ggatcatcct tggcctgctt gtcctctggg gcgttggcgg ctacaacgga
     2881 cttgtcagct cgcgcgaagg cgtgaacaaa gcctgggcga atgtcgagac gcaataccag
     2941 cgtcgttccg accttatccc gaacctggta gcaaccgtca agggtgcggc cgactttgaa
     3001 aagtccaccc tcgaagcggt gacgcaggcg cgcgcgaacg cgaccagcat caagatcgat
     3061 ccgaacaacc tgaccccgga aaaactccag gagttccagc aagctcaggc ccaattgggc
     3121 ggtgcgcttg gccgactcct tgccgtagcc gagaactacc cgcaactgcg cgcggtgcag
     3181 aactttaccg acctgcaggc gcagttggaa ggtaccgaaa atcgtatcaa cgaagcgcgt
     3241 cgtcagtaca acgaagctgc ccgtgattat aacatttccc gtgcgaagtt tccccgggtg
     3301 atcctcgcga acctgttcgg cttcaaggac cgtccgtact tcgaagctga caaaggcgcc
     3361 gagaaagctc cggaagtgaa attctgatgg cctccgacgg acgcttcacc gatgccgagc
     3421 accggcagat cctcgacgcc attggggcgg ccgagcgact gacctccggc gagatccgac
     3481 tgtttgtgga agatgtatgc ggcgagaacg tactcgatcg cgccgcctac atctttcacg
     3541 aattgaaaat ggaccgcacg gcggagcgga atggcgtcct
//
LOCUS       DAOTON010000202         3579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20681_length_3579_cov_2.537741, whole genome shotgun sequence.
ACCESSION   DAOTON010000202 DAOTON010000000
VERSION     DAOTON010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20681_length_3579_cov_2.537741"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="P5292_05140"
     CDS             <1..586
                     /locus_tag="P5292_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRS38547.1"
                     /translation="AYIRVANDQGMLEMALVENIQRQDLNPIDVAISYKRLIEECSLT
                     QEQLSERVGKNRTTVTNFVRLLKLPPEIQAALRDNRITMGHARAILGVDDIGHQLLLF
                     KEILEKQLSVRDVEERARQASGKRSQRENQSASGSGRSASQQQFEYEKIRKVLSSHFS
                     AKVQLSHQSDGSGKITIPFGSDEDLNRILELLNY"
     gene            595..1191
                     /locus_tag="P5292_05145"
     CDS             595..1191
                     /locus_tag="P5292_05145"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HRS38548.1"
                     /translation="MTASRIYSRSPFPAFRPCSTALLLLLLFHVTLQTSAQERTDQDS
                     LSVLQNKRANRAALSSALLPGAGQIMNKKYWKLPILYGGLGALVYFAGTNQQYYKDYK
                     SAYAARIDTDPLTVDAYPRLSASDLQVRKDYYRRNRDLCYILGGVVYILNIVDAYVDS
                     QLRDFDVSDQLSLSIRPGLLDSRQAVPAAGFRFTLTIP"
     gene            1195..1938
                     /gene="dapB"
                     /locus_tag="P5292_05150"
     CDS             1195..1938
                     /gene="dapB"
                     /locus_tag="P5292_05150"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRS38549.1"
                     /translation="MNIALIGYGKMGKTIERLAEERGHRIVLKVDREEIAALTPEALK
                     AAQVAIEFTQPDAAVANIYRCFDAGIPIVCGTTGWYEHKADVDARCKLGGHTLFTASN
                     FSIGVNLLFAINERLAELMNRQPAYREVLIHETHHLQKLDAPSGTAISLAEQIVGRLD
                     RLHAWHAYRHDESVTAGPESKGELPVFSSREDDVPGTHVVKYVSEADELELIHKAYNR
                     TGFATGALMAAEWVVGKRGVFGMKDLLSL"
     gene            2033..3199
                     /gene="lepB"
                     /locus_tag="P5292_05155"
     CDS             2033..3199
                     /gene="lepB"
                     /locus_tag="P5292_05155"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRS38550.1"
                     /translation="MARFRINWDLIKDIVFGIFTVGVWFLLKPKPGQRKSATREWIDA
                     IIFAVIAATIIRTFLIEAYTIPTPSMEKSLLVDDFLFVSKVSYGARVPMTPLSFPFAH
                     NTLPLVGGKSYLEWIKIGYHRLPGFGSVHRGDCVVFNWPAENEGRPVDKKENYIKRCV
                     GIAGDTIEIRDQQVYIDGQSQPLPEQAQSSYHVITDGTGFSEQAVQRLGLRQSGEQIS
                     PVEYSFHMTKTGAEEMKHFGNVKLVEQNLIPKSAYQDFIFPFDPRFGWNVDNFGPLWI
                     PKKGATIKIDSSNIALYRKVIADYEGNQLEERNGQILINGSPATSYTFKMDYYFMMGD
                     NRHNSADSRFWGFVPEDHVVGKAVMIWMSLDRNASFFNKVRWGRLFHFIGGYGG"
BASE COUNT          878 a          948 c          922 g          831 t
ORIGIN      
        1 ggcctacatc cgcgtagcga atgatcaagg tatgctggaa atggccctgg tcgaaaacat
       61 ccagcggcag gaccttaacc cgattgatgt ggcgatcagc tacaagcgac tgatcgaaga
      121 gtgcagcctc acccaggaac aactcagcga acgggttggg aagaaccgca cgacggtcac
      181 caatttcgtc cgcctgctca aattgccgcc tgagatccag gccgcactgc gcgacaaccg
      241 gatcacgatg ggacatgcac gtgcgattct cggagttgac gacattggtc atcaactgct
      301 gcttttcaag gaaatcctgg aaaagcaatt gagtgtccgc gacgtggaag aacgtgcccg
      361 ccaggcttcc ggaaaacgaa gccagcgtga aaatcagagc gcatcaggtt ccggtcgctc
      421 cgcttcgcag cagcagttcg aatatgaaaa gatccgaaag gttctttcca gtcatttcag
      481 cgccaaagtt cagttaagcc atcagtcaga cggttccggt aagatcacca ttcccttcgg
      541 gagcgacgaa gaccttaacc gcatcctcga actgttgaac tattgatcta ccggatgacg
      601 gcaagcagga tctactcccg atcgccgttc ccggcattcc gtccctgctc cactgccctg
      661 cttctgctgc tgttgtttca tgtaacactg cagacttcgg cacaagaacg gactgaccag
      721 gattcacttt cggttcttca gaacaaacgg gccaaccggg cggccttgtc atcggccctt
      781 cttccgggcg ccggccagat catgaacaag aagtactgga agttgccgat cctctatggc
      841 ggactcggag cgctcgtata cttcgcaggt accaatcagc agtattacaa ggactacaaa
      901 agcgcctatg cagcgcggat tgatacggat cccctgacgg ttgacgcgta cccccgactg
      961 agtgccagcg atcttcaggt aaggaaggat tactatcgtc gtaaccgcga tctctgttat
     1021 atcctgggcg gtgttgttta catactgaat atcgtcgacg cgtatgtgga ctcccagcta
     1081 cgcgactttg acgtctccga ccagttgtcg ttatccatcc gtccgggatt gctggatagt
     1141 cggcaagctg ttcctgcggc gggttttcgt tttaccctga caattcccta aaccatgaat
     1201 atcgcgttga tcggatacgg taaaatggga aaaaccattg agcgactggc ggaagaacgc
     1261 ggtcatcgta tcgtgcttaa agtggaccga gaggagatcg cagcactcac acccgaagcg
     1321 ctaaaggctg cgcaggtcgc gatcgagttt acacaacccg atgccgcggt cgcgaacatc
     1381 taccggtgtt tcgatgcagg gatcccgatc gtttgtggta ccaccggctg gtacgagcac
     1441 aaagcggatg tggatgcccg ctgcaaactg ggtggacata ccctttttac agcctccaac
     1501 ttcagcatcg gggtcaacct tctgttcgcc atcaacgaac ggttagccga gctcatgaat
     1561 cgtcaaccgg cctaccgcga agtgctcatt cacgagactc accatttgca gaagctcgat
     1621 gctccgagcg gtaccgcgat ctcgctggcc gagcagatcg tcggtcgcct cgaccgcttg
     1681 cacgcctggc atgcctatcg gcatgatgag agcgtaactg caggtccgga aagtaaaggc
     1741 gaactaccgg tgttctcttc ccgggaagac gatgttccgg gcacccatgt ggtgaaatac
     1801 gtctccgaag ccgatgaact cgaactcatc cacaaggcct acaaccggac cggcttcgcc
     1861 acaggtgccc tgatggctgc cgaatgggtg gtcggcaagc gcggggtgtt cggcatgaag
     1921 gacttattaa gtctttgatg tgtaaatttt aacaccccat taaccgccaa gggggcaagg
     1981 cggggctacc ttcgcacgtt aacaattcgc tccacgcgaa aaacaattca atatggcacg
     2041 attccgcatc aattgggacc ttattaaaga cattgtcttc ggcattttca ccgtcggtgt
     2101 ctggttcctg cttaaaccca aaccggggca acggaagtcc gccacacgag aatggatcga
     2161 cgccatcatc ttcgctgtca tcgcggctac gatcatccgg actttcctga tcgaagccta
     2221 taccatcccg actccatcga tggaaaaatc cctcctggtg gacgattttc tgttcgtcag
     2281 caaggtgagc tatggcgccc gggtaccgat gacgccgctc tcgtttcctt tcgcgcataa
     2341 taccttaccg ttggttggcg gcaaatccta tctcgaatgg atcaagatcg gttatcatcg
     2401 cttaccggga ttcggcagtg ttcatcgcgg agattgcgtg gtcttcaact ggcctgctga
     2461 aaacgaagga cgaccggtcg acaagaaaga aaactacatc aaacgctgtg tcggcattgc
     2521 cggcgacacc attgagatcc gggatcagca ggtgtacatt gacgggcaat cacaacccct
     2581 gccggagcag gcacaatcga gttaccacgt cattaccgat ggcacgggct tcagcgaaca
     2641 ggccgttcaa cgacttggct tgcgtcagag cggcgagcag atctcaccgg ttgaatacag
     2701 tttccacatg acgaaaacgg gtgctgaaga aatgaagcac ttcgggaacg taaaactggt
     2761 ggaacaaaat ctgatcccga aatcggctta tcaggacttc attttcccgt tcgacccccg
     2821 cttcggttgg aatgttgaca atttcgggcc gctgtggatt ccgaaaaaag gcgcgaccat
     2881 caagatcgat tcttccaaca ttgcgttgta ccgaaaggtg attgccgatt acgaaggcaa
     2941 tcaactcgaa gagcggaacg gacagatcct tatcaatgga agcccggcaa cgagctatac
     3001 cttcaagatg gattactatt tcatgatggg cgacaaccgc cacaattcag ccgactcacg
     3061 cttttggggt ttcgttcccg aagaccacgt agtcggtaaa gccgtcatga tctggatgag
     3121 cctcgatcgg aatgcgtcct tcttcaacaa ggtacgctgg ggacggttgt ttcactttat
     3181 cgggggatat ggaggctagt aactggtaac tggtaactgg taactggtaa ttagtaatta
     3241 gtaattagta attagtaatt ggtaattggt acgttgggat tgtaaactag aaattagtaa
     3301 ctagttacta gtttctagtt actagtttct agttactagt ttctagttac tagtttctaa
     3361 ttaccaatta ctcctgactt acgattgaca attacttttt ctgtatccgc cctcaattaa
     3421 aagctttctt caggctaatc ccagcagaaa ttctaccgtg tcaacgccat cggcgtagtc
     3481 ccagagggat gggaactggg tagtgccgaa gcggacaaag gataaggtgt caggatttcc
     3541 ggctttcaag gcctcttccg tattcttcga gcagacaag
//
LOCUS       DAOTON010000203         3573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20713_length_3573_cov_2.787095, whole genome shotgun sequence.
ACCESSION   DAOTON010000203 DAOTON010000000
VERSION     DAOTON010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20713_length_3573_cov_2.787095"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="P5292_05160"
     CDS             complement(<1..591)
                     /locus_tag="P5292_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS38551.1"
                     /translation="MMTNRYKTMIVDDEPAQLESLTGMLGRFPSYELVASCSSVPEAL
                     RVLAEQQPDLLLLDVMLPPDTGFDLLEKLQERSFEVIFTTSHADFAIRALREAAVDYL
                     LKPVGEDVLRDALFRFEQRFREKEKSELRRLDILLNNLQQLDKGQRTLALPTNFGFHC
                     LRVDEILRCESDGNYTTVYALEGKPILICRTMKEFED"
     gene            complement(617..1441)
                     /locus_tag="P5292_05165"
     CDS             complement(617..1441)
                     /locus_tag="P5292_05165"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRS38552.1"
                     /translation="MSVSNPLLLLSTPADTVTLVFLLLVPVVVIFLFMVFVIYRIRRE
                     QQVRQREAELREQTAEMEMKALRAQVNPHFIFNSLQSIHLFIQKHDHEHAGKYLLKFS
                     GLIRLVLENSEHKEIPLAEELKALELYMQLEQLRVRNGFDYHFDIADGLDPEAVYVPP
                     LLLQPFVENSIWHGLHHRASKGAIWLRFGKRAHMLEIELKDNGAAAPDNVDPGAQPMN
                     SEKRKSMGTSITRQRLELLNRSRAAQSVIQLEDIRDAEGTYAGKRVFVRIPLIEED"
     gene            complement(1452..1691)
                     /locus_tag="P5292_05170"
     CDS             complement(1452..1691)
                     /locus_tag="P5292_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38553.1"
                     /translation="MESANHPKYTGANDTGLTIDIYGGLWTGAAGLTNGREERTNGLP
                     VLPESGSRCNFEEGREQPSSGTEWGWAVPLSGSSV"
     gene            complement(1809..2795)
                     /locus_tag="P5292_05175"
     CDS             complement(1809..2795)
                     /locus_tag="P5292_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRS38554.1"
                     /translation="MEYRKLGRSGLQVSALSLGSWLTFGKQIGDDVAEQLMHAAYDAG
                     VNFFDNAEIYARGQSEIVMGNILARSGWDRSSYLVSSKAYFGTEGAKSKPNQRGLSRK
                     HLVEACEAALRRLQVDYLDLYFCHRPDKQTPIEETVWTMHQLVLQGKILYWGTSEWDA
                     SEIMEAMLVAERHHLIAPVMEQPQYNLLTRGKLERGYLHLFRYHGIGTTIWSPLASGV
                     LSGKYLGASDEDTRLKMKGLEWLREQVLREEVMTKVKSLKALADECETTLPLLSIAWC
                     LKNPHVSTVILGASRLSQLQENLGALDVVPRLTQERMDRIDQIFPKPILPEY"
     gene            2837..3355
                     /locus_tag="P5292_05180"
     CDS             2837..3355
                     /locus_tag="P5292_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38555.1"
                     /translation="MKRYCSLLFAMIALVVQANAQSLSPSVIASGGGFSANGTAMLSF
                     TTGELTMVETFSAGSSILTQGFQQPEDFSTGISVVTAQGPGMSAFPNPATDAINLLSD
                     FTEAGRLRLLLTDALGRIVLQGDELEIPGGTSRISQDVSRVASGVYFVHAVFQTHSGK
                     ATASIQKITIQH"
     gene            3367..>3573
                     /locus_tag="P5292_05185"
     CDS             3367..>3573
                     /locus_tag="P5292_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Vir protein"
                     /protein_id="HRS38556.1"
                     /translation="MFRNLVLSAIFLCLAGASIAQAPQALNYQAVARDGTGNLLVNQN
                     ASVRYSVHDGSAGGTVVYSETHSVT"
BASE COUNT          781 a         1071 c          919 g          802 t
ORIGIN      
        1 gtcctcgaac tctttcatgg tccggcagat gagaatcggc tttccttcca gcgcatagac
       61 cgtcgtgtag ttaccgtccg attcgcagcg caggatctcg tcgacccgca gacaatggaa
      121 cccgaagttg gtcggtaacg caagggttcg ttgccccttg tcgagttgct gcagattgtt
      181 caacaggata tccaggcgcc gcagttccga tttttctttc tcacgaaagc gctgctcgaa
      241 ccggaagagc gcgtcccgca atacatcctc gccgaccggc ttcaacaggt agtcgaccgc
      301 ggcttcccgt aaggcccgga tggcgaaatc ggcatgcgag gtggtgaaga tcacttcgaa
      361 ggaacgctcc tgcaatttct ccaacaggtc gaaaccggta tccggtggca gcatcacgtc
      421 gagcaagagc aggtccggtt gctgctccgc caatacgcgc aaggcttccg gaacgctgct
      481 gcagcttgca acaagttcgt acgacggaaa tcgtcccaac atcccggtca acgattcgag
      541 ctgcgccggc tcgtcgtcga caatcatggt cttgtagcgg ttagtcatca tgtcaggatc
      601 gcaaataacg ggttgattaa tcctcctcga tcaacgggat acgcacgaat acccgtttcc
      661 cggcatacgt tccttcggca tcgcggatgt cttcgagctg aatcaccgac tgtgccgcgc
      721 gggaacgatt cagcagctcc aaacgctgcc gggtaatgga tgtacccatg gatttacgct
      781 tttccgaatt catcggctgt gccccgggat cgacattgtc cggtgccgca gctccattgt
      841 ctttaagctc gatctccagc atgtgggctc gcttaccgaa ccgcagccat atcgcgcctt
      901 tcgaagcacg atggtgcaag ccatgccaga tggaattttc tacgaagggt tgcaacaaca
      961 agggcggaac gtacacggct tcaggatcca atccgtccgc aatatcgaaa tgataatcga
     1021 agccgtttct gacgcgcaat tgttccaatt gcatatacaa ttcaagagct tttaactcct
     1081 ccgccagggg tatctctttg tgctcggagt tttccagcac cagccggatg agtccggaga
     1141 acttcaggag atacttaccg gcatgctcgt gatcgtgctt ttggataaag aggtggatgc
     1201 tctgcaggct gttgaagatg aagtgcggat tgacctgcgc gcgcagcgct ttcatctcca
     1261 tctcggccgt ttgttcccgc agttctgctt cccgttgccg cacctgctgc tcacggcgaa
     1321 tccggtaaat gacgaatacc atgaacagaa aaatcacgac taccgggacc aacaataaga
     1381 agacaagggt caccgtgtcg gcaggcgtcg ataaaagcag aagcgggtta gaaacagaca
     1441 caacgaaatc ctcaaacact cgagccggac agcggtaccg cccaacccca ttccgttccg
     1501 gatgagggct gttcccgtcc ctcttcgaaa ttacaacggc ttcccgattc cggcaataca
     1561 ggcaagccgt tcgttcgctc ttcccgaccg ttcgtcagcc cggcagcacc ggtccataga
     1621 ccgccgtaaa tatcgattgt gagtcccgta tcattggctc cggtatactt cggatggttc
     1681 gcagactcca aaggttgcct gaacagttta ccccttttgc agcacgaact ccaactttcc
     1741 acattttaag catgataacg gggaggcact ccaacctccc cgtcatcttt aatatcggtt
     1801 taatccgatc aatactccgg aagaatcggt ttggggaaga tctgatcgat acgatccatc
     1861 cgctcctgcg tcagtcgggg tacaacgtcc agcgcgccca ggttctcctg taactggctc
     1921 agccgggacg cgccaaggat caccgtgctg acatgcggat tcttcaggca ccaggcgatg
     1981 gataacaacg gcagggtggt ttcgcattca tccgcgagcg ccttcaacga tttcactttc
     2041 gtcatgacct cttcgcgcaa gacttgctcg cgcaaccatt cgaggccttt catcttaaga
     2101 cgggtgtctt catcggaggc gccgaggtat tttccggaca agaccccgga ggcaagcggc
     2161 gaccaaatgg tcgtaccgat accatggtaa cggaagaggt gcagataccc ccgctccagc
     2221 ttgccccgcg tcagcaaatt atactgcggt tgctccatca ccggcgcgat caggtggtgt
     2281 cgctcggcca ccagcatcgc ttccatgatc tcggacgcgt cccattccga agttccccaa
     2341 tacaggatct tcccttgcag taccaattgg tgcatggtcc aaacggtttc ttcgatgggc
     2401 gtctgcttgt cgggacgatg acagaaataa aggtccaggt aatcgacctg cagtcgccgg
     2461 agcgccgctt cacaggcttc gacgagatgc ttgcgcgaca aaccgcgctg gttgggtttc
     2521 gacttcgctc cttccgttcc gaaataggct ttcgaagaaa cgaggtacga actccggtcc
     2581 cagccggatc gcgccaggat gtttcccatc acgatctccg attgtccgcg cgcgtagatc
     2641 tccgcattgt cgaagaagtt cacacccgcg tcgtaggccg cgtgcatcaa ctgttccgct
     2701 acatcgtcgc cgatctgctt accgaaggtg agccaggaac ccagcgacaa cgcgctgact
     2761 tgtaaacctg accgtccaag ctttctgtat tccatatatc aatattatct atcttggttg
     2821 aaataaacca tttcgcatga aaagatattg ctcactcctg ttcgcgatga tcgcgctggt
     2881 ggtccaggcg aacgcgcaat cactttctcc gagcgtcatc gcgtccggcg gcgggttctc
     2941 cgcgaacgga acggcgatgc tgtcgttcac gaccggtgaa ctgacgatgg tggaaacctt
     3001 ctcggccggc tcgtcgatcc tgactcaggg attccagcaa ccggaagatt tcagcaccgg
     3061 catctcggtc gtcaccgcac aaggtcccgg catgtcggcg ttcccgaacc cggcgaccga
     3121 tgccatcaac ctgctgtcgg acttcaccga agccggacgc ttgcggctgt tgctgaccga
     3181 cgcccttggg cgtatcgtcc tgcagggcga cgagctcgaa attcccggtg gaacatcccg
     3241 cattagccag gacgtttcgc gcgttgcttc gggtgtctat ttcgttcatg ccgtctttca
     3301 aacgcattcc ggcaaagcaa ccgcttccat tcaaaagata accatccaac attaatcgcc
     3361 aacccaatgt tcaggaacct cgtactttct gccatctttc tatgcttagc cggcgcttcg
     3421 atcgcgcagg ctccgcaagc gctcaactat caggcagtcg cccgtgacgg caccggcaac
     3481 ctgctggtca accaaaacgc cagcgtacgc tactccgtac acgacggcag cgccggcggt
     3541 acggtcgttt attcggaaac acattcggtc acg
//
LOCUS       DAOTON010000204         3566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20765_length_3566_cov_2.737397, whole genome shotgun sequence.
ACCESSION   DAOTON010000204 DAOTON010000000
VERSION     DAOTON010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20765_length_3566_cov_2.737397"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="P5292_05190"
     CDS             <1..596
                     /locus_tag="P5292_05190"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRS38557.1"
                     /translation="GAWFSVNVQKRVTRTFSLNVNPQVRFNRNLATVDQYMAELGGEF
                     LITRNFRATLNYRLTESNRLDYFSTRHRVFLDLSYKQKYGRVVIGLRERIQSQFKDVY
                     SSDLGRYPAWYLRTRLSLKYDTDLSWKPYASTEIFYRTRDLENPGNSVDQYRYEVGID
                     YSITQDQSLNVFYMFKRNPEDRVYNEYITGLGYSITF"
     gene            complement(597..1073)
                     /gene="ispF"
                     /locus_tag="P5292_05195"
     CDS             complement(597..1073)
                     /gene="ispF"
                     /locus_tag="P5292_05195"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRS38558.1"
                     /translation="MRIGFGFDVHQLKEDHPFWLGGIRIPHTKGALGHSDADVLIHAI
                     CDALLGAAALGDIGKHFPDTSTEFKNIDSKILLRRVRDLIDSKGWQIGNIDATLCLER
                     PKIAGHIPAMLESLCGVLEIPASDLSIKATTNEKMGYVGREEGVTAYAVALLLKKA"
     gene            complement(1181..2416)
                     /gene="porV"
                     /locus_tag="P5292_05200"
     CDS             complement(1181..2416)
                     /gene="porV"
                     /locus_tag="P5292_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010603321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HRS38559.1"
                     /translation="MNFSRKKAVFAALAAGVFSLPAKAQISSFRDSLLGQINTITTAV
                     PFLQIAPDSRAGGMGDYGVATSPDANSIHWNAAKLAFVEKNVGFSISYTPWLRALVND
                     INLAYISGYKKLKDDQAIAGSLRYFSLGNIDFTNDNAEKLGSFNPNEWALDFAYSRKL
                     GENFSGGLALRYIYSNLTGGIAIQNNTIQTRPGRSVAADVSGYYNKKIDLSNKDAILA
                     FGLNISNIGAKISYTDTKAKDFIPMNFRLGSNLKVDLDSYNTISFGVDINKLLVPTPP
                     VYWKNSIGSDSLDAAGNKVIRAGKDPSNISPAQAIFSSWSDAPAGFEEELKEFTYSIG
                     AEYWYDKQFAIRAGYFHEAATKGNRKYFTIGAGLRYNVFGLDFAYLIPTTQRNPLENT
                     LRFTLIFDFEGLKKEAAEE"
     gene            complement(2469..>3566)
                     /locus_tag="P5292_05205"
     CDS             complement(2469..>3566)
                     /locus_tag="P5292_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38560.1"
                     /translation="QYEVVTSTINGVPANLPTDTLKALSRITVTGEVRGLNGSRLTSF
                     NGTLYPTVYDKTETIYTLGNDINVTNDPSFPAPFQLRKNVIFRGKVSVTNGTFSFTFI
                     VPRDIRFQYGYGRISYYAQNGQTDAHGYNNDIVVGGYNSLAAADSKGPEIRLYMNDEK
                     FVRGGLTDANPYLYAIVSDTSGINTIGNGIGHDMTAELSTETNKIHVLNDYFESDLNS
                     YQNGKVYYPFSELSPGPHTVTFKVWDVYNNSSESSTDFIVAESADLALSHVLNYPNPF
                     TTRTTFMFEHNRPYTNLDVQVQVFTVSGKLIKTISGKIFSTGYRSDDLHWDGLDDFGD
                     RIGRGVYVYKLRVRASDGSYADKFEKLVILR"
BASE COUNT          796 a          883 c         1001 g          886 t
ORIGIN      
        1 ttggcgcctg gttcagtgtt aacgttcaga aacgcgtcac ccgcacgttt tcgctgaacg
       61 tcaatccgca ggtgcgtttt aacaggaacc tggctaccgt tgaccaatac atggcggagc
      121 ttggcggcga attcctcatc acgcggaact ttcgcgctac actgaactac cgcctgaccg
      181 agagcaaccg gcttgattat ttttccacgc gtcatcgcgt attccttgat ctgagttata
      241 agcagaagta cggaagagtg gtcatcggac tccgggaacg cattcagtcc caattcaagg
      301 atgtctattc tagcgacctt ggaagatatc cggcctggta cctgcgtacc cgcttgtcgt
      361 tgaaatacga taccgatctt tcctggaagc cctatgcgag taccgagatc ttctaccgga
      421 cccgtgacct ggaaaatccc ggtaattcag tggatcaata ccgatacgaa gtggggattg
      481 attactccat cacccaggac caaagcctga acgtgttcta catgttcaaa cgtaatccgg
      541 aagaccgtgt ttacaacgaa tacatcacgg gtctcggcta cagcatcact ttttaatcag
      601 gcttttttca gcagcagtgc taccgcgtaa gcggttacgc cttcttcgcg tcctacgtac
      661 cccatctttt cattcgtggt ggctttgatc gaaaggtccg aagcgggaat ctccagtact
      721 ccgcaaagcg attccagcat ggctgggatg tgcccggcga tcttgggtcg ttcgaggcaa
      781 agcgttgcat cgatgttccc gatctgccag cctttgctgt cgatcaggtc gcgtacccgc
      841 cgcaggagga tcttactgtc gatgttcttg aactcggtgg acgtgtctgg aaagtgtttg
      901 ccaatgtcgc ccagggccgc agcgcccagc aatgcgtcgc atatggcgtg aatgagtaca
      961 tcggcgtccg aatgaccgag agctcctttc gtatggggta tccggatgcc gcccagccag
     1021 aatgggtggt cttccttcaa ttggtgaacg tcgaatccga aaccgatacg catggatctt
     1081 gcttttcccc aaagttaatc aatcgactgc agacctatcc taaatgcaag aggccgtgct
     1141 gttcaacagc acggcctctt gagtatggtt gggaagaagc ttattcttca gcagcttctt
     1201 ttttcaatcc ttcgaaatcg aagatgagcg tgaagcggag tgtgttctcc aacgggttgc
     1261 gttgcgtcgt cgggatcagg taggcgaagt caaggccgaa cacattgtaa cgaagtccgg
     1321 caccgatggt gaagtactta cggttgccct tggtagccgc ttcgtggaag taaccggcac
     1381 ggatggcgaa ctgcttgtcg taccagtatt cggcgccgat tgaatacgtg aattctttca
     1441 actcttcttc gaaaccggcc ggagcatccg accaggaact gaagatggct tgcgccggag
     1501 agatgttgga ggggtctttg cccgcgcgga tcactttgtt tccggcggcg tcaagactgt
     1561 cggagccgat ggaattcttc caatagaccg gaggagtggg tacgaggagc ttgttgatgt
     1621 ctacaccgaa cgagatagtg ttgtagctgt cgaggtcgac tttcagattc gatcccaggc
     1681 ggaaattcat cgggatgaaa tctttcgctt tggtgtcggt ataggagatc ttggcaccga
     1741 tgttcgagat gttcaaaccg aaggccagga tagcatcctt gttggaaagg tcgatcttct
     1801 tgttgtaata gccggaaacg tcggcagcga ccgaacggcc gggacgcgtc tggatcgtgt
     1861 tgttctgaat ggcgattccg ccggtcaggt tcgagtagat ataacggagt gcaagaccac
     1921 ccgagaagtt ctcgcccagt ttgcgtgaat acgcgaagtc caacgcccat tcgttcggat
     1981 taaaggaacc gagcttttcg gcattatcgt tggtgaagtc gatgttaccc agggagaaat
     2041 aacgaagcga acccgcgatc gcctgatcgt ccttcagctt cttgtagccg gagatatacg
     2101 ccaggttgat gtcgttcacc agggcgcgca gccagggggt gtaggagatg gagaaaccga
     2161 cgttcttttc gacgaaggca agtttagcgg cattccagtg aatggaattg gcgtcagggc
     2221 tcgtcgcgac accgtaatcg cccatgccac cagcgcggga atcaggtgca atctgaagaa
     2281 aaggaaccgc cgtggtgatc gtattgatct gcccaagcaa cgagtcccgg aaggaactga
     2341 tttgcgcttt ggccggaagg ctgaatacac ctgcggccaa ggctgcaaaa acagcctttt
     2401 ttctgctaaa attcatgagt tcagtgtaag ggtcggcaaa tatacgtgct ggtacccaaa
     2461 atggttcctt aacgtaaaat caccaatttt tcgaatttat ccgcatagga accgtcgctt
     2521 gcgcgaaccc ggagtttgta aacgtaaact ccgcgaccga tccggtcgcc aaagtcgtcg
     2581 aggccatccc agtgcaggtc atcggaacga taaccggtcg agaatatttt gccgctgatc
     2641 gtttttatca atttcccgga aaccgtgaat acctgaacct ggacatccag gttggtgtac
     2701 ggccggttgt gttcgaacat gaacgtggtt cgcgtggtga acggattggg ataattgagt
     2761 acgtgactca aagccaggtc ggccgattcc gccacgatga aatcggtact ggattcgctg
     2821 gagttgttgt aaacgtccca gaccttgaaa gtgaccgtgt gcggacccgg gctcagttcg
     2881 gaaaaggggt aatagacttt tccgttctgg tagctgttga ggtcgctttc gaaatagtcg
     2941 ttgagcacgt ggatcttgtt ggtttcggtg ctcaactccg cggtcatgtc gtgacctatt
     3001 ccgttgccga tggtattgat tccgctggtg tcgctgacga tcgcgtagag gtatggattg
     3061 gcatccgtca ggccgccgcg aacgaacttc tcgtcgttca tgtacaaccg gatctccggc
     3121 cctttggaat ccgcggcagc aagtgaattg tatccgccta ccacgatgtc gttgttatag
     3181 ccatgcgcgt cggtttgtcc gttctgggca tagtaactga tgcgtccgta gccgtattga
     3241 aaacgaatgt cgcgcggaac aatgaaagtg aaggagaaag tgccgttcgt cacgctgacc
     3301 ttcccgcgga agatcacgtt ctttctcaac tggaacggag cggggaaact ggggtcgttc
     3361 gttacgttga tgtcgttgcc cagcgtgtag atcgtttccg tcttgtcgta aacggtcggg
     3421 tagagtgtgc cattaaacga ggtaagccga cttccattga gtccacgcac ttctccggtg
     3481 accgtgatcc ggctaagggc tttcagggtg tcggtaggca ggttggcggg aacgccgttg
     3541 atcgtactcg taactacctc gtactg
//
LOCUS       DAOTON010000205         3564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20780_length_3564_cov_3.923055, whole genome shotgun sequence.
ACCESSION   DAOTON010000205 DAOTON010000000
VERSION     DAOTON010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20780_length_3564_cov_3.923055"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="murG"
                     /locus_tag="P5292_05210"
     CDS             complement(<1..1055)
                     /gene="murG"
                     /locus_tag="P5292_05210"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRS38561.1"
                     /translation="MPRHDELRVIISGGGTGGHIFPAVSIAKALKEADSSTEILFVGA
                     EGRMEMEKVPAAGFKIVGLPIRGIQRSLSVSNLTLPWKVLQSLLKARKIIREFRPDVA
                     VGVGGYASGPLLFMATRMGIPALIQEQNSFAGLTNRWLAKRVNKICVAYEGMEKYFPS
                     DKILLTGNPVRPDIQYIEGKRAEALQFFGLSPDKPVLLAIGGSLGARTINETLENDLE
                     RLAGAKVQLIWQTGKSFFSRAQACAKQFEQQGIKAFDFIARMDLAYAAADLVVSRAGA
                     SSVSELTLTGKAAILVPSPNVAEDHQTKNAMALVKHDAAVLVRDQDARESLVREALTL
                     LSDGERRRLLSERIAVLA"
     gene            complement(1042..2205)
                     /locus_tag="P5292_05215"
     CDS             complement(1042..2205)
                     /locus_tag="P5292_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRS38562.1"
                     /translation="MQSTWLDKYFRGDRTIWLVVFILSIFSLLAVYSSAGSLAYRYKD
                     GNTEYYLFKHLAMLLFGIGLMYAAHLLKYTVYSRVSQFALIIAVPLLILTLALGTNLN
                     EASRWLTIPGINITFQTSDFAKIALIMYVARVLSKKQDEITSFRSAFLPVVLPVLLVC
                     GLILPANFSTAAVLFASCLFLMFIGRIRLKFIFSLIGLGIAGFGLYLLIAIATGNKGR
                     IDTWMARIERFSDEEHKDNYQADQSKIAIATGGIVGKFPGNSVQRNFLPHPYSDFIYA
                     IIIEEYGLIGGVLIVFLYLLLFYRVVRFLHHSPMAFGTLVAVGCTFTLVFQAMINMAV
                     AVNLFPVTGQPLPLVSMGGTSIIFTSVAVGIVLSVSRQVEKEKLEGGDELATA"
     gene            complement(2239..>3564)
                     /gene="murD"
                     /locus_tag="P5292_05220"
     CDS             complement(2239..>3564)
                     /gene="murD"
                     /locus_tag="P5292_05220"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRS38563.1"
                     /translation="LVVLGAAESGVGAAILAIRKGFNVFVSDMGVIKEEYRRMLEQNG
                     IPFESGQHTESLILNADEVVKSPGIPDKAELIKKLHAAGIPVISEIEFAGRYTRAKII
                     GITGTNGKTTTTMLMYHMLKKAGFNVGLGGNVGKSFALQVAENDFDYYVLELSSFQLD
                     GIRDFRCHIAILTNITPDHLDRYEYKLENYVRSKFRITMNQHPDDCFIYCADDPITRE
                     YMETVGFQARLLPFSIKSTVEQGAFLENGNTLTIHINQDHFSMFIHELALQGKHNLYN
                     SMAAGIAARVLEIRKETVRESLSDFRNVEHRLEHVTTVHGIEFINDSKATNINSTWYA
                     LESMTKPVVLILGGVDKGNDYESLLELVREKVKGIVCLGKENKKIVKTFKEVVPVVEA
                     LSADEAVQASYRMAKKGDAVLLSPACASFDLFENYEDRGRQFKKAVRAL"
BASE COUNT          822 a         1014 c          935 g          793 t
ORIGIN      
        1 gcaagtacgg cgatccgttc cgacaggagg cggcgccttt ccccatcgga aagcaaggtc
       61 aaggcttccc gtaccagcga ttcccgcgca tcctggtcac gcaccaacac cgcggcatcg
      121 tgcttcacca gggccatggc gttcttggtc tgatgatctt ccgctacgtt gggcgaaggc
      181 acaagaatcg cggcttttcc ggttagcgtt agctccgata cagaactagc tcccgccctg
      241 gagacgacca ggtcggcagc cgcgtaggcc aggtccatcc gcgcgatgaa gtcgaacgcc
      301 ttgatgcctt gctgctcgaa ctgtttggcg catgcctgcg cgcgactgaa gaaggatttc
      361 ccggtttgcc agataagctg aaccttcgcc cccgcaagac gttccagatc attttccagc
      421 gtttcattga tggtccgggc accgaggctt ccacctatgg ccagtaacac cggcttatcc
      481 ggtgacaatc cgaaaaattg caaagcttcc gcacgcttcc cttcgatgta ttgaatatcg
      541 ggccggaccg gattgccggt cagcaggatc ttatcggacg ggaagtattt ttccattccc
      601 tcgtaggcca cacatatttt attcacacgc ttcgccagcc atcggttcgt gagaccggcg
      661 aaggaatttt gctcttgtat caaggcgggt atgcccatcc gggtcgccat gaacaacaac
      721 ggcccgctgg catatccgcc tactcccact gcaacatccg gacggaattc acgaatgatc
      781 ttccgcgcct tcagcaggct ttgcaacacc ttccacggca aggtcaggtt tgaaacgctc
      841 aggctgcgct ggatgccgcg aatcggcaaa ccgacgatct taaaccctgc tgccggtact
      901 ttttccatct ccatccgtcc ttccgctcca acgaagagta tctcggtcga cgaatccgct
      961 tccttgagcg ctttggcgat cgagaccgcg gggaatatat gcccgccggt cccgcctccg
     1021 ctgatgatca cccgcaactc atcatgccgt ggcaagctcg tcccctcctt ccaacttttc
     1081 cttttcgacc tggcgactaa cacttagtac gatacctact gccacactgg taaagatgat
     1141 ggaggtaccg cccatactga ccaggggcaa gggttgtccg gtgaccggaa acaggttcac
     1201 ggcaacggcc atgttgatca tggcctggaa taccagggtg aacgtgcatc cgactgcgac
     1261 cagcgtaccg aatgccattg gactatggtg caggaagcgg accacccggt agaataacag
     1321 caaatacaaa aacacgatca acacgccacc gatcaacccg tactcttcaa tgatgatcgc
     1381 atagatgaaa tccgaatagg gatgcggcag gaaattccgc tgaacggaat tacccgggaa
     1441 tttgccgaca atccccccgg tggcaatggc gatcttggac tggtcggcct ggtagttgtc
     1501 tttgtgttcc tcatcactga atcgctcgat gcgggccatc cacgtgtcga tccttccctt
     1561 attcccggtc gcaatggcga tcagcagata aagtccgaat ccggcaattc cgagtccgat
     1621 cagcgagaag atgaacttca accggatcct tccgatgaac atcagaaaca agcaggaagc
     1681 aaagagcacg gcggctgtac tgaaattggc cggcaggatc aatccgcata ccaacagcac
     1741 cggaagcacg accggcagga aggccgaccg gaaactggtg atctcatcct gcttcttcga
     1801 caatacgcgg gcgacgtaca tgatcaacgc gatcttggcg aagtcggaag tctggaaggt
     1861 gatgttgatt cccggaatcg tcaaccaacg gcttgcttcg ttcagattcg tccccaacgc
     1921 gagtgtcagg atgagcaaag gcactgcgat aatcagcgcg aattgcgata ccctcgaata
     1981 gacagtatac ttcaacaaat gcgccgcata catcaggccg ataccgaaca acagcatggc
     2041 caggtgcttg aagagatagt actccgtatt tccgtcctta taccggtagg caaggctgcc
     2101 ggcactgctg tatactgcca gcagcgagaa gatcgagagg ataaaaacga ccaaccagat
     2161 cgtccggtcc ccacggaagt acttatcaag ccaggtggat tgcatcaggt aagatgtatt
     2221 cggtgaaatg aatggtgatc aaagtgcgcg aacggccttc ttgaactgac ggccacggtc
     2281 ctcgtagttc tcgaagagat cgaagctggc acaggcaggc gacaacagca ccgcatcgcc
     2341 tttcttggcc atccgatagg aagcttgcac agcctcgtca gcggaaagcg cttccactac
     2401 cggaactact tccttgaagg tcttgacgat cttcttattc tctttgccaa ggcaaacgat
     2461 ccccttcact ttctcgcgaa caagttcgag cagggattcg taatcgttgc ctttgtccac
     2521 gccgcccagt atcagaacga ccggtttggt catcgactcc agggcgtacc aggtggaatt
     2581 gatgttcgtc gctttcgaat cgttgatgaa ctcgataccg tgaacggtgg taacgtgttc
     2641 gagacgatgt tcgacgttcc ggaaatccga caggctttcg cgaacggttt ccttacggat
     2701 ttccaatacg cgggcggcga tgccggcggc catggagttg tagagattgt gtttcccttg
     2761 cagggcgagc tcgtggatga acatagaaaa gtggtcttgg ttgatgtgta tggttagtgt
     2821 gtttccgttt tcaagaaagg cgccctgctc caccgtcgat ttgatggaga atggaagcag
     2881 ccgggcctgg aagccgactg tctccatata ttcccgggta atcggatcgt ccgcacagta
     2941 aatgaaacag tcgtcgggat gctgattcat ggtgatcctg aacttggagc ggacataatt
     3001 ctccagcttg tattcgtagc ggtccaggtg atccggtgtg atattggtca ggatggcgat
     3061 gtggcagcgg aaatcgcgga tgccgtcgag ctgaaagctg ctcaactcca ggacgtaata
     3121 gtcgaagtca ttctccgcaa cctgaagcgc gaagctcttg cccacatttc cgccgaggcc
     3181 tacattgaaa ccggccttct tcaacatgtg atacatgagc atggtggtgg tcgtcttccc
     3241 gttggtgccg gtaatgccga tgatcttcgc acgcgtataa cggccggcga attcgatctc
     3301 gctgatgacc ggaatacccg ccgcatgcaa cttcttgatg agctccgcct tgtcaggaat
     3361 accgggactt ttcaccactt cgtccgcatt caggatcagt gattccgtat gctgcccgga
     3421 ttcgaacggg atcccattct gctccagcat ccggcggtac tcctctttga tcacgcccat
     3481 gtcggagaca aacacattga accccttcct gatagccaga atggcggcac cgacaccgct
     3541 ttcagcggcg cccagcacca ccag
//
LOCUS       DAOTON010000206         3564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20782_length_3564_cov_3.137646, whole genome shotgun sequence.
ACCESSION   DAOTON010000206 DAOTON010000000
VERSION     DAOTON010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20782_length_3564_cov_3.137646"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="P5292_05225"
     CDS             <1..1411
                     /locus_tag="P5292_05225"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38564.1"
                     /translation="IPQNLHVDFACPDTMQIGWNAVTGATAYEVSVLGSRYMDSVMTV
                     TGTTAQLAIPATNEVWFSVRALVGNGKGRRANAVQKAPGLVSCSLADDIGLSTMIFPV
                     EGNLYPCFPLNSVPVTVEIRNDGLNTASGFQLSYTINGGSPVTETYTGSLIHGATTNF
                     TFAVPADFSAGGAFNLVVTVNATPDLNLANNELDINLQVVAAGAAAPLQEAFAPAFPP
                     AGWDVIASGPDYEWSVKTNITGSDGSNTDAAWFDNYSYNNSGALDYLVTILVDFAAVA
                     YPQLLFDVAYAQFSGYEDGLRIEQSADCGATWTATGYEKIGAALASVPSSNTDWSPSS
                     GNDWRNDTVELTSLAGELALIRFVNINDFGNNLYIDNINLQNNNVLSSGVPVRIPALA
                     IFPNPGTGVYTVDLRDLPLGSVQLTLTDVSGRLIESRNLMNNGRVTEQLNLLNEPVGV
                     YILQVRTAEKVYSMRLTKI"
     gene            complement(1480..1989)
                     /locus_tag="P5292_05230"
     CDS             complement(1480..1989)
                     /locus_tag="P5292_05230"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HRS38565.1"
                     /translation="MKRLLQLLQDAERSPIRLWLLNRIAARAIPFNAPHGIRILKITP
                     DGIRSFLPYRRRNLNHLKGLHACSLATLGEFTAGMSLLHALRSMDYRLIMKEIKVTYH
                     RQGKSDARAECRLDETWLRQHVKEPLHSSDAVFVPIQVPVTDESGESLCTVEVHWQVK
                     RWDKVRTQS"
     gene            complement(1993..3006)
                     /locus_tag="P5292_05235"
     CDS             complement(1993..3006)
                     /locus_tag="P5292_05235"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRS38566.1"
                     /translation="MRIENDLTRMLNLRLPLVMAPMFLVSNEAMIREAIGAGILGCFP
                     SLNFRKEGELEGVLERLNQFLQTRQGQPGTYGVNLIVQRSNFLFERHLKVCVDRKVPV
                     YITSLGNPTETIQAAHAYGAKVFCDVTNLTHAEKCARNGCDGFIAVGQGAGGHAGPYP
                     LSLLVRTLKSHFPDIPVLAAGGIADGSGILSALASGASLAYCGTRFIATSEAPVGQPY
                     KDAVIQSGMDQIVLTERISGTPCTVIRTPFVEKIGLKQNRFERWLGKNPRTRKYFKML
                     VQKRGFNWLEEAVKPGNYNNLWCAGQSVELIHDIVPVRTIVDRYESELNEAWQALSAT
                     IRP"
     gene            3242..>3564
                     /locus_tag="P5292_05240"
     CDS             3242..>3564
                     /locus_tag="P5292_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRS38567.1"
                     /translation="MEIKRLFDIPRRQLDRFPKEDALTAKENGKWTPYSTRYFVDMID
                     RVSYGLLASGIGKGDKIAIISANRPEWNFVDHGMMQIAAVNVPIYTTLSEPEIKFILN
                     DCGAK"
BASE COUNT          783 a          969 c          966 g          846 t
ORIGIN      
        1 tatcccacaa aaccttcacg tggattttgc ctgcccggat accatgcaga tcggatggaa
       61 tgccgtaaca ggcgcaacgg cgtatgaagt cagtgtgctg ggttcgcgct acatggactc
      121 cgtaatgacc gttaccggca ctaccgccca acttgccatt cctgctacca atgaagtttg
      181 gttcagtgta agagcacttg tcggaaatgg caagggacgc agagccaatg ccgttcagaa
      241 ggctcccggg ctggtcagtt gcagccttgc cgacgatatc ggccttagca cgatgatctt
      301 cccggtggaa gggaacctct atccttgttt cccgctcaac agtgttccgg ttacggtcga
      361 aatacgcaac gatggcttga atactgcctc aggcttccag ttgtcatata cgatcaacgg
      421 cggctcgcct gttaccgaga cgtacacggg ttcactgatt catggcgcta ctacgaactt
      481 tacgtttgcg gtcccggccg atttctccgc gggcggagca ttcaatctgg tcgtgacggt
      541 taacgccacc ccggatctga acctcgccaa caacgaactg gacatcaacc tccaggtagt
      601 agcagccgga gccgcggcgc ctttgcagga agcgtttgct ccagcattcc cgccagccgg
      661 ttgggacgtg atcgcatccg gacctgatta tgaatggtcg gtcaaaacca atatcaccgg
      721 atctgacggt tcgaataccg atgctgcctg gttcgataat tacagttaca acaattccgg
      781 cgcactcgat tacctggtga ccatcctggt ggacttcgcg gcggtcgctt atccgcagtt
      841 gctgttcgat gtcgcctatg cccaattctc cggttacgaa gatggcttgc ggattgagca
      901 atccgccgat tgcggcgcta cctggacggc taccggttat gagaagatcg gtgcagcact
      961 ggcttccgtg ccttcgagca ataccgactg gtcgccttcc tccgggaacg attggagaaa
     1021 tgacacggtt gaactgactt ccctggccgg agaactggcg ctgatacgct ttgtcaacat
     1081 caacgatttt ggcaataacc tttacatcga caacatcaat ctccagaata acaatgtgct
     1141 ttcttcggga gttccggtcc gtattccggc actcgcgatt ttcccgaacc ccggaaccgg
     1201 agtgtacacc gtcgatctcc gcgatctgcc gcttggttcg gtacaactga cccttaccga
     1261 tgttagcggt cgactgatcg agtcgcgcaa cctgatgaat aacggcaggg taacggagca
     1321 attgaatttg ctaaacgagc ccgtcggcgt ctacatcttg caggttcgta cggctgaaaa
     1381 ggtctacagc atgcgcctga cgaaaatctg atcctggcta ttcatactgc aaggcccggc
     1441 aggagcgaag agcgctgccg ggccttgctg tttacgccat taggactgag tccggacctt
     1501 atcccagcgc ttgacctgcc agtgtacttc gaccgtgcaa agtgattcgc cgctttcatc
     1561 ggttacgggt acctggatgg gtacgaacac cgcgtcagat gagtgaagcg gttccttcac
     1621 gtgctgacgc aaccaggttt cgtcaagcct gcattccgcc cgcgcgtccg atttgccctg
     1681 acggtgatag gttaccttga tttctttcat gatcaaccgg tagtccattg atcgaagcgc
     1741 atgcagcaac gacatgccgg cggtgaattc gcccaacgta gccaatgaac aggcgtgtaa
     1801 cccttttaag tgattcaggt tccttcgccg atagggcagg aaggaacgaa tgccgtccgg
     1861 agtgatttta aggatgcgga tgccatgcgg tgcattgaac ggaatggcgc gagccgcaat
     1921 gcggttcagt aaccacaacc ggatcggtga gcgttccgcg tcttggagaa gttgcaacag
     1981 ccttttcatg gatcagggtc ggatggtagc ggaaagggcc tgccaggctt cgttcaattc
     2041 ggattcatac cggtccacaa tggtcctgac cggaacgata tcgtggatca gctcgacgct
     2101 ttggccggca caccacagat tgttgtagtt gccgggcttg accgcttctt cgagccagtt
     2161 gaaaccgcgt ttttggacca gcatcttgaa gtatttgcgt gtccgtggat tcttgcccag
     2221 ccatcgttcg aaccggtttt gtttcagtcc gatcttctcg acgaagggcg tgcgaatcac
     2281 cgtacagggt gtcccgctga tgcgctccgt aagaacgatc tgatccatgc cggattggat
     2341 cactgcatct ttgtagggct gtccgaccgg agcttcggat gtggcaatga aacgggttcc
     2401 gcaataggcc agggaagcgc ccgatgcgag ggcgctcagg atgccgctac cgtctgcaat
     2461 gccaccggct gccagtaccg gaatgtcggg aaagtgcgac ttcagcgtgc gcaccaggag
     2521 cgaaagcggg taggggccgg catgtccgcc ggcaccctga ccgacggcaa tgaacccgtc
     2581 gcatccgttt cgggcacact tttccgcgtg ggtcaggttg gtgacgtcgc aaaagacttt
     2641 ggcgccataa gcatgtgccg cctggatggt ttctgtcgga ttacccagcg aggtgatgta
     2701 taccggaact ttccggtcga cacaaacttt gaggtgacgt tcaaagagga agttgctccg
     2761 ctgaacgatc aggttgactc cataagtacc cggctgaccc tggcgcgttt gcaggaattg
     2821 gttcaagcgt tcgagtactc cttccagttc gccttccttt cggaaattca ggctgggaaa
     2881 gcagcccaga atacccgccc cgatggcctc ccggatcatc gcctcgttac taaccagaaa
     2941 catgggtgcc atgaccaacg gaagtcgcag gttgagcatc cgggtcagat cgttttcaat
     3001 tctcatagtc gttcaaattt cggctattga agccttgagg aatgggatac tgctgggtaa
     3061 ttttcaacga tttcggctgc atgaccgttc atcacagtgc aaagtgcctg atggaatccc
     3121 gaaatctcct ttccagccct caacagttga cagaattact tgttaataat cgggacaatc
     3181 ctgcgttttt tacgcgcttt taccccctct tgaatatgta aattcgtaac gcgcattttg
     3241 catggaaatt aaacgtctgt tcgatattcc acgccgtcaa ttggatcgtt ttcccaagga
     3301 ggacgccctg acggccaagg aaaacgggaa gtggactccg tattctaccc ggtatttcgt
     3361 tgatatgatc gaccgggtga gctacggttt actcgcatcc ggaatcggta aaggcgataa
     3421 gatcgccatc atctcggcca accggcctga atggaatttt gtcgaccacg gcatgatgca
     3481 gatcgccgcg gtcaacgtcc cgatctatac gacgcttagc gaaccggaga tcaagtttat
     3541 tctgaacgac tgcggcgcaa aaat
//
LOCUS       DAOTON010000207         3548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20880_length_3548_cov_2.884054, whole genome shotgun sequence.
ACCESSION   DAOTON010000207 DAOTON010000000
VERSION     DAOTON010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20880_length_3548_cov_2.884054"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="P5292_05245"
     CDS             <1..929
                     /locus_tag="P5292_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38568.1"
                     /translation="METESGVATAFWTPRGEPMALLPNHQRALYASGQPLLQERQIVN
                     RQPKQEFSWHLTATTGAFQVFKNVESDFKPWDDIRNKREQEIVTGQVGVAVGVGRRSW
                     DATIGVELTQYGERNEYVPRSYQDQYIDDSYWQAYTYWSVDTDTAYIMGNPWMMNTDV
                     QMTDSNWVASYDTVNQLSYDPSIDAARTRTSIRYIEFPLDFTWRLSSNRWGFGITAGF
                     APAWLSSSRGRYISRSLRGSESMQELGELRQFLLNARFGLEAEYRMHPNVTLLVRPQY
                     RQQFGSVFKEGSGISQRYQAVGVQAGVRYRFR"
     gene            1130..2308
                     /locus_tag="P5292_05250"
     CDS             1130..2308
                     /locus_tag="P5292_05250"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRS38569.1"
                     /translation="MEAYIIAAYRSAVGKAPRGKFRTIRPDDLGVAILRHLVASVPQL
                     DKELIEDVIVGNATPEAEQGLNMGRMISLMGLDTVKVPGMTVNRYCASGLETIAIAAA
                     KIKAGLADCIVAGGVECMSPIPFGGWKIVPNYKYVQSNPDYYWGMGLTAEAVARDYKV
                     SREDQDLFGFESNRKAVAAIKEGRFKDDIVPVTVEETYLDENEKRKTRSFVVDTDEGP
                     RADADVSKMAQLKPVFDAKGTVTAGNSSQTSDGAAFVLVVSERMLKQLGVQPIARFVS
                     YAVSGVEPRVMGIGPVEAIPSVLRKSGLKQSDIDLIELNEAFASQSLAVMRTLDLDPA
                     KVNVNGGAIALGHPLGCTGSKLTVQVLNELRRRKQRYGMVTMCVGTGQGAAGIFELLN
                     "
     gene            2444..>3548
                     /locus_tag="P5292_05255"
     CDS             2444..>3548
                     /locus_tag="P5292_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRS38570.1"
                     /translation="METVSKQAIKGGEFLIKTTPAEQVFIPEEWSEEQKMIAQTCHDF
                     LVAEVYPNLDRIDAQEEGLMQKILDKAGELGLLGISIPEEYGGFGKDFTTGMIATEFL
                     GAGHSFSVAYAAHTGIGTLPILFYGNDAQKKHYLPKLTSGEWKAAYCLTEPGSGSDAN
                     SGKTKATLSPDGKHWIINGQKMWITNGGFADVYIVFAKIDNDENLSAFIVEKGTPGLS
                     LNPEEHKMGIKGSSTRQVFFSDCQVPVQNQLGERGIGFKIAVNILNLGRIKLAGAAIG
                     GCKLTSTKSIQYANERTQFGRPIAKYGAIRHKLAEQAIRIFACESAIYRCSQNIEDAI
                     EALIAGGMDPAKAKLKAVEQFAVEAAILKVHGSE"
BASE COUNT          839 a          905 c         1010 g          794 t
ORIGIN      
        1 aaatggagac ggagtcaggc gttgctacag cgttttggac gccaagaggc gaacccatgg
       61 cccttttacc caaccatcaa cgggcattgt atgcttctgg tcagccgctc ttgcaggaac
      121 ggcaaattgt taaccgccag ccgaagcagg agttttcctg gcacctcacg gccaccacag
      181 gcgccttcca ggtattcaag aacgtcgagt cggattttaa gccttgggat gacattcgaa
      241 acaagcggga acaggagatc gtcacaggac aagttggggt agcggttgga gttgggagac
      301 gatcctggga tgctaccatc ggcgtcgagt tgacgcaata cggtgagcgg aacgagtatg
      361 tgccacgcag ttatcaggac caatatatcg atgacagcta ctggcaagcc tatacgtatt
      421 ggtcggtgga tacggacacc gcctacatca tgggcaatcc gtggatgatg aacacggatg
      481 tacaaatgac cgacagcaac tgggtggcct cctatgatac ggtgaatcag ctttcctatg
      541 acccttccat tgatgcagcc cgtaccagga cgagcatccg gtatatcgaa ttccctcttg
      601 attttacctg gcggctttcc agcaaccgat ggggattcgg tataactgcc ggttttgcac
      661 ctgcatggtt gtcctcctcc cgcggccgtt acatttcgcg ctcgctgcgt ggttcggagt
      721 ccatgcagga gcttggtgaa cttcgtcagt tcctgctcaa tgcccgtttt gggcttgagg
      781 cggaatatcg gatgcatccg aacgttaccc tgctggtccg tccacagtac cggcagcaat
      841 tcgggtcggt attcaaggag ggatccggca tttcccagcg atatcaggct gtcggtgtgc
      901 aggcaggagt tcgttaccgt tttcgttagt gtgttttagg tgtgtgtaga agtgggggtc
      961 tccgtatggg ggctcccgct tcattttttc aacgaattgc cgattcggct gtgtaatgtt
     1021 ttgtggaatt caatgaaaac ttagcaggtc ctgtacttaa atttactatc ctgaaaatga
     1081 tgcccggctg accggtcgga tctttcatca tttcaaatca ttgtcgacta tggaagctta
     1141 catcatcgcc gcttatcgat ccgccgttgg gaaggcaccc cggggtaaat tcagaaccat
     1201 acgtcccgac gacctcggcg tagccatctt gcgtcatttg gttgcgtcgg ttccgcaatt
     1261 ggataaggaa ctgatcgagg atgtgatcgt cggcaacgca acaccggagg ccgagcaggg
     1321 tttgaacatg ggtcgtatga tttccctgat gggactcgac accgtcaagg tgccgggcat
     1381 gaccgtcaat cgctattgcg cttcgggttt ggaaaccatt gccatcgcag ccgctaagat
     1441 caaggccggg ctggcagatt gtatcgtggc cggtggagtg gagtgtatgt ccccgattcc
     1501 cttcggaggc tggaagatcg tgcccaacta caagtatgta cagtcgaacc ccgattacta
     1561 ttggggtatg ggccttacgg ccgaagcggt cgcgcgtgat tacaaggtgt cgagagaaga
     1621 ccaggacctt ttcggctttg agtcgaacag gaaagccgtg gcagccatca aggagggccg
     1681 gttcaaggat gacattgttc cggtaaccgt ggaagagacc tacctcgatg aaaatgaaaa
     1741 gcgaaagacc cggagttttg tcgtcgacac cgacgaagga ccgcgggccg atgcggacgt
     1801 ttcgaagatg gcacaattga agccggtgtt tgatgcaaaa ggaaccgtaa cagccggcaa
     1861 ttcctcccag accagcgacg gtgctgcctt cgtgctcgtg gtcagcgagc ggatgctgaa
     1921 acaactgggg gttcagccca tcgcgcgttt tgtcagctac gcagtctcgg gcgtcgaacc
     1981 gcgtgtcatg ggcattggac cggtggaagc catcccttcc gtactccgga aaagcggatt
     2041 gaagcaatcg gacattgacc tcattgaact caacgaagct ttcgcctcgc aatccctggc
     2101 ggtaatgcgg actctggatc tggatccggc aaaggtcaat gtaaacggcg gagctatcgc
     2161 gctgggacat ccgctgggct gtaccggttc gaaactcacc gtccaggtct tgaatgagct
     2221 caggcgcagg aaacaacgct atggcatggt taccatgtgt gtcgggaccg gtcagggtgc
     2281 tgctgggatt ttcgagttgc tcaactaagg ccgggatgta agaaataccg ctgttttgtt
     2341 cggtatttcg actatttacc gggcttcaca gttgccatca gcagaacact ccgggaggtt
     2401 tattcgttag aacagaggac agaacagact cattttttaa atcatggaaa cagtaagcaa
     2461 gcaagcgatc aaaggaggag agttcctcat caagacaacc ccggccgaac aggtattcat
     2521 ccccgaagag tggtcggaag agcagaagat gatcgcgcag acttgccacg acttcctggt
     2581 cgcggaagtg tacccgaacc tggatcgaat cgatgcccag gaggaaggat tgatgcagaa
     2641 gatcctggac aaggccggcg agttgggact gttgggcatt tccatcccgg aagaatacgg
     2701 cggctttggt aaagacttta ccaccggtat gatcgcaacg gaatttctcg gcgcgggaca
     2761 ttccttttca gtggcttacg ccgcgcatac gggcatcggt actttgccga tcctgttcta
     2821 tggaaacgac gcgcagaaaa aacactacct gccaaagttg acctccggcg agtggaaagc
     2881 agcctattgc ctgaccgagc cgggatccgg atccgatgcc aactccggta aaacgaaagc
     2941 aacactctcg cccgacggca aacactggat catcaacggt cagaagatgt ggatcaccaa
     3001 cggcggcttc gccgatgtct atatcgtctt cgccaagatc gataacgatg agaacctgag
     3061 cgcgttcatc gttgaaaaag gcactcccgg tttgtccctt aatccggaag aacacaagat
     3121 gggcatcaag ggcagttcga cccgtcaggt gttcttttcc gactgccagg tgccggtcca
     3181 aaaccagctc ggagaacgcg gtatcggttt caagatcgcg gtgaatatcc tgaatcttgg
     3241 tcgtatcaag ttggccggag ccgccatcgg cggttgcaaa ctgaccagca caaaatcgat
     3301 ccagtacgcc aacgagcgta cgcaattcgg acgtccgatc gccaagtatg gcgccattcg
     3361 gcataaactc gccgaacagg cgatccgaat cttcgcttgt gaatcggcga tctaccgttg
     3421 cagtcagaat atcgaggatg ccatcgaagc cctcatcgcc ggtggcatgg atcccgctaa
     3481 agctaaactc aaagcggttg agcagtttgc cgtagaagcg gctatcctga aagtgcacgg
     3541 aagcgagc
//
LOCUS       DAOTON010000208         3542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20911_length_3542_cov_3.344996, whole genome shotgun sequence.
ACCESSION   DAOTON010000208 DAOTON010000000
VERSION     DAOTON010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20911_length_3542_cov_3.344996"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="P5292_05260"
     CDS             complement(<1..226)
                     /locus_tag="P5292_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRS38571.1"
                     /translation="MKIRVQSIHFDADRKLIDFIQDKVDKLVTFYDHIIGGEVFLKLD
                     KSSDMKNKIVQIKIQLPGNDIIVSEQCKTFE"
     gene            complement(258..1133)
                     /locus_tag="P5292_05265"
     CDS             complement(258..1133)
                     /locus_tag="P5292_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRS38572.1"
                     /translation="MIQGFLQYIRYEKRFSPHTVQAYSTDLEQYALFLRSTYETDDLS
                     AATHSMIRSWIVQLMEQKITTRSINRKLTTLKTYYKFLLRQGTLRVNPMLRVQAPKTS
                     KRLPAFVDERKMDQLFTEVAFPEGFTGLRDRLVLETLYGTGMRLAELVGLKDSDVDLH
                     AMQLKVLGKRNKERVIPFTDKMRELIVAYRQVRNGHFASREKFFVTDQGKSLYPKFVY
                     RLVQRYLGVITTLEKRSPHVLRHTFATHMLNRGADINSVKELLGHANLSATQVYTHNT
                     IEKLKKVYQQAHPRA"
     gene            complement(1264..1458)
                     /gene="rpsU"
                     /locus_tag="P5292_05270"
     CDS             complement(1264..1458)
                     /gene="rpsU"
                     /locus_tag="P5292_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRS38573.1"
                     /translation="MIIVPIKENESIDKALKKFKKKFERTGVLRELRRRQTFEKKSVA
                     RRQQVLRAKYRDLMQRQETL"
     gene            complement(1513..2652)
                     /locus_tag="P5292_05275"
     CDS             complement(1513..2652)
                     /locus_tag="P5292_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRS38574.1"
                     /translation="MNFQPTENQVLIAETVRKFAETHIRPDMMKWDEEQLFPVELFRK
                     MGEQGLMGILVPHEYGGSGLSYFEYMTAIVEVARVCGSIGLSMAAHNSLCTGHILAFG
                     NEQQKQQYLPKLASGQWIGAWGLTEANTGSDAMRMQCVAKQDGDHWVINGSKNWITHG
                     KSGDVAVVLARTGDLLDSKGITAFIVERGTQGFKAGKKENKLGMRASETAELIFEDCR
                     IHKDQVLGNVGDGFVQAMKVLDGGRISIASLALGIAKGAYDAALKYSRERQQFGKPIA
                     DFQAIGFKLADMATKIEAAELLTLQAADLKNRGEKCTKQSAFAKYYASEVSVQVSTDA
                     VQIFGGYGYTKDFPVEKFYRDSKLCTIGEGTSEIQKLVISREILK"
     gene            complement(2688..3218)
                     /locus_tag="P5292_05280"
     CDS             complement(2688..3218)
                     /locus_tag="P5292_05280"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRS38575.1"
                     /translation="MDVVSRICTAIRDIPDFPQPGILFKDITPILLDPALCSDIVGAI
                     HQDFSGQKIGGIVGVESRGFLFGMMLAQRFQVPFIPIRKKGKLPYRTVSYEYKLEYGS
                     ATMEMHEDAIEPGTRILVHDDLLATGGTAAAAAELIRHLRGEVAGFSFVVSLDFLNGR
                     DKLNPYGAPVKALVSY"
     gene            complement(3262..>3542)
                     /locus_tag="P5292_05285"
     CDS             complement(3262..>3542)
                     /locus_tag="P5292_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRS38576.1"
                     /translation="EVWGVDDSLYSFIVRRLTVDESGLHRKLYLNTDSLERFGRHPYL
                     GWKLGRILINYRKQHGPYQHVEELLKTGLIEEDIFRKLAPYLEVGYSE"
BASE COUNT          802 a          924 c          897 g          919 t
ORIGIN      
        1 cttcaaaggt cttgcattgc tcgctaacga tgatatcatt gcctggaagt tggatcttga
       61 tctgcacgat cttgttcttc atatcggaag atttgtccag cttcagaaat acttctccac
      121 cgatgatatg gtcgtaaaag gtcaccagct tgtccacttt atcctggatg aaatcgatga
      181 gtttacggtc ggcgtcgaaa tggatggatt gaactctgat cttcatagtg ctttcttctt
      241 cttttttaaa cgttcgctca ggctctcgga tgagcctgct ggtatacttt tttaagtttc
      301 tcgatggtat tgtgcgtata cacctgcgtt gcactcaggt tggcatgtcc cagcagttct
      361 tttacactgt tgatgtctgc tcccctgtta agcatgtgtg tggcgaaggt gtgtcgcagg
      421 acgtgaggac tgcgtttctc cagtgttgtg atgactccaa gatagcgctg gaccagccgg
      481 taaacgaatt tagggtagag ggattttccc tgatcggtca cgaaaaattt ttcccgggat
      541 gcaaaatggc cgttccggac ctgccgatac gcaacgatca actcccgcat cttatcagta
      601 aacggaatga cgcgctcttt gtttcttttc cccaggactt tgagctgcat cgcgtggaga
      661 tcgacatccg aatctttcaa ccctaccaat tcggccaaac gcatgccggt tccgtaaagg
      721 gtctccaaca ccaggcggtc ccgaagaccc gtaaagcctt caggaaacgc gacttcggtg
      781 aagagctggt ccattttccg ttcatcgacg aaggcgggaa gccgcttcga tgtttttgga
      841 gcctgcaccc gcagcatcgg gttgacacgt aacgtcccct gacgaagcag gaatttataa
      901 taggttttta atgtggtcag cttccggttg atggaacggg tcgtgatctt ctgctccatc
      961 agttggacga tccaggaacg gatcatcgaa tgtgttgctg ccgacagatc atcggtctca
     1021 taagtcgatc gcaggaaaag ggcatactgc tccaggtcgg tactgtacgc ctggaccgtg
     1081 tgtggcgaga accgcttttc gtagcggatg tattgaagga atccctgtat caccggcaat
     1141 aaaaaaagag aacctcccga gtgggggaag ttctcttgct atgctttctt taccatcatc
     1201 gcattcgtgt tacgaaacga ctaccagtaa agatacaaat tccggatgga tacagcgagc
     1261 gaatcagagc gtttcctggc gctgcatcag gtcgcggtat ttcgcgcgaa ggacctgctg
     1321 gcggcgggca accgactttt tctcaaaagt ctggcggcga cgcaattcac gcaaaactcc
     1381 cgtacgttcg aatttcttct tgaacttctt gagggccttg tcgattgact cgttttcttt
     1441 gatcggaacg atgatcatgt tgttcttttg tattggggtg gcgaaattac aaaatcctga
     1501 actaaaagca aattacttta ggatttcgcg ggaaataacc agtttttgaa tctcggaggt
     1561 tccttccccg atggtgcaca gtttggagtc ccggtagaat ttctcaacag ggaaatcctt
     1621 ggtataccca taaccgccaa agatctgtac agcgtccgtg gagacctgta cagagacttc
     1681 cgacgcgtaa tatttggcaa aagcgctttg ttttgtgcat ttctcacccc gattcttcag
     1741 gtcagccgcc tgaagggtca ggagttcggc cgcctcgatc ttggtggcca tatcggccag
     1801 tttgaagcca atagcctgaa aatcagcgat cggtttgccg aattgctggc gctcccggga
     1861 gtacttcaag gccgcgtcgt acgctccttt ggcgatcccc agggcaaggg aggcaatgga
     1921 aattcggcct ccgtcgagga ctttcatcgc ctgtacgaat ccgtcgccta catttccgag
     1981 gacctggtcc ttgtggatgc ggcaatcttc gaagatgagc tccgcggtct cggaagcacg
     2041 catgcccagc ttattctctt ttttacccgc cttgaagccc tgggttccac gctccacgat
     2101 gaaggctgtg atgccttttg agtcaagcag gtctcctgtc cgagccaata cgaccgcaac
     2161 atccccactc tttccgtggg tgatccaatt cttgctacca ttgattaccc agtgatcacc
     2221 gtcctgcttc gctacgcatt gcatgcgcat cgcatcgctg ccggtattgg cttctgtcag
     2281 gccccaggct ccgatccact gtccgctggc aagcttggga aggtactgtt gcttctgctg
     2341 ctcatttccg aacgccagaa tatgaccggt gcaaagtgag ttatgcgccg ccatgctcaa
     2401 tccgatggaa ccgcagacac gcgctacctc aacaatggcc gtcatgtatt cgaaatagct
     2461 caaacccgat ccaccatatt cgtgcggtac caggattccc atcagcccct gctctcccat
     2521 ctttcgaaac aactcgaccg ggaacagttg ctcttcgtcc cacttcatca tgtcggggcg
     2581 aatatgggtc tcggcgaact tgcgaacggt ttcggctatc agtacctggt tctcggtagg
     2641 ttggaaattc ataggatcaa taacaacagc taagtggtct gtagagttca ataagatacc
     2701 agcgctttaa cgggagcgcc gtatgggttc agtttatccc gaccgttgag aaagtcgagg
     2761 ctaactacga atgaaaagcc ggcgacttca ccgcgcaggt gacggatcaa ctcggcggcc
     2821 gcggccgcgg tcccgccggt tgccagcaga tcatcgtgca ccaagatgcg tgtacccggc
     2881 tcgatcgcgt cttcgtgcat ctccatcgtt gcactgccgt actcgagttt gtattcgtat
     2941 gaaacggttc gatacggaag cttccctttc ttgcgaatgg ggatgaaggg cacctggaat
     3001 cgctgggcga gcatcatgcc gaagagaaag ccccggctct ctactcctac tataccgccg
     3061 atcttttgtc cggaaaaatc ctggtgaatg gctccgacga tatccgaaca caaagcaggg
     3121 tcgagcagaa tcggcgtgat atccttgaac aggattccgg gttgcggaaa atcgggtata
     3181 tcgcgaattg cggtacagat acgggatact acgtccatgg attgggtgcg ggacgcttct
     3241 aaataattga ctaacaatta tttattcact gtacccgact tccagataag gcgcaagttt
     3301 acggaaaatg tcctcttcta tcaacccggt tttcagtagt tcctccacgt gttgataggg
     3361 accgtgctgc ttccggtaat tgatcaggat gcggcccaac ttccaaccga ggtaaggatg
     3421 ccggccgaag cgctcgagtg aatccgtgtt caagtacaac ttgcgatgaa gccccgattc
     3481 atcaaccgtc aagcggcgta cgatgaatga atacaagctg tcgtcaacac cccaaacctc
     3541 ct
//
LOCUS       DAOTON010000209         3537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20941_length_3537_cov_3.144170, whole genome shotgun sequence.
ACCESSION   DAOTON010000209 DAOTON010000000
VERSION     DAOTON010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20941_length_3537_cov_3.144170"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..933)
                     /locus_tag="P5292_05290"
     CDS             complement(1..933)
                     /locus_tag="P5292_05290"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS38577.1"
                     /translation="MESMVDFKEASYKAHQDGRKFSGKSAELVMDKSTVDYWRHERMY
                     STLLPLLQHYPGASWITIGDGNFGKDANYLLSQGADATATDLNNPSYDLALELGFIRK
                     FKIENAEHLTFGDDSFDFALVKEAFHHFPRPYIALYEMLRVAKKGVVLIEPNDQDTIS
                     LPDLNLKNGFFWFVRTLKKSLSRILRPSDKPALDYTLFYEPAGNFVFTLNYHELKKVA
                     IALNYPAIAWKGFNDHYEEGAAEERLVDRGPLYQKIAAGIRRRDRQVSKGTNFPTMIT
                     FVIFKQQPAAATVSTLKAAGYVFEPLPRNPYIGN"
     gene            complement(1010..1864)
                     /locus_tag="P5292_05295"
     CDS             complement(1010..1864)
                     /locus_tag="P5292_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38578.1"
                     /translation="MKLHKRVLLVASVLLSAATACKQTSTDSASSQDTLPTIEDTMSK
                     EQKELVDFKFFFTLANLPSPMELINDIYKNEVPFNSDLLNSTDHEEKYTSAYKKSVNY
                     GIYGIDMAYAAFYGQNQDLLSYYSTTKALAEKMNVQETFDRYTESFQKNSDNQDSLVR
                     MIDKAYAETDNYLRNNGRYMTAAHVLAGAIIEVQHLSIALMKDQERTPGNAAIFEKIF
                     NQRMYLDNLVNLFKEFEKDPDSGKLLKDLQELQAAFNAVKSADELTKDRLTNLLVTVD
                     KVRNRMTN"
     gene            2434..3396
                     /locus_tag="P5292_05300"
     CDS             2434..3396
                     /locus_tag="P5292_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HRS38579.1"
                     /translation="MFLLLLVASGASYYVANRGKTTVKSEVVPVPAGNDVKMNRNTAF
                     RFIRPLLFTDYSLEDEKLMPLKGDIIRYIEGQKSSGNIQSASVYLRRLNSASWMTINP
                     KEEYPVASLFKVPVMIAILMTADQDPSFLNRKIFYPEFPAGVFQHNVAPLPTNKHYTI
                     RQLLESMVARSDNEAYDLLWKAMDQRNFNKMLFDFQLKSFQIQDTTDYSLSVVDYSRF
                     LRVLYNGGYLSHERSEIALDILSKCDYKEGLIRDLPADLISSRKFGYRVKPDLAALSE
                     FGVFYLKGEPYLLGVMTKGLNYNHLTETLAHISKLVYNSQLPNS"
BASE COUNT          848 a          894 c          874 g          921 t
ORIGIN      
        1 ttaatttccg atgtagggat tacggggtaa cggttcgaat acgtagccgg cagctttcaa
       61 tgttgaaacg gtggcggcag ctggctgttg tttgaaaatg acaaaggtga tcatggtcgg
      121 aaagtttgtt cccttcgata cctgacgatc ccgtctccgg atgcctgccg cgatcttctg
      181 atacaagggg cctcgatcaa ccaaccgttc ttcggcagcg ccttcctcat aatggtcatt
      241 gaaccctttc caggcgatgg ccggatagtt cagggcgatg gcaaccttct tcaactcatg
      301 atagttgagc gtaaaaacga agtttcctgc cggttcatag aaaagagtat aatcgagtgc
      361 gggtttatcc gaagggcgca agatccggct aaggcttttc ttaagcgtac ggacgaacca
      421 gaagaaacca tttttcaggt tgagatccgg taaggagatc gtatcctgat cattgggttc
      481 gattaacacc acaccctttt tcgccacccg gagcatctcg taaagcgcga tgtacgggcg
      541 gggaaaatga tgaaacgctt cttttaccaa tgcaaaatca aagctatcgt caccgaatgt
      601 caggtgttcc gcgttttcga tcttgaattt ccgaatgaat cccaactcga gcgcaagatc
      661 ataagaagga ttattcaggt ctgttgcggt cgcatcagca ccttgactta gcagataatt
      721 tgcatccttt ccgaaatttc cgtcgccgat cgtgatccaa cttgcacccg gataatgttg
      781 gagaagcggc agcagggtgc tgtacatccg ctcatgccgc caatagtcga ccgtggattt
      841 atccatcacc aactctgcag acttgccgga gaatttccga ccgtcctgat gtgctttata
      901 gctggcttct ttgaaatcaa ccatggactc catacaggtg atctggcctg atccggacaa
      961 cccgacaggt actgccggtt atttcaggat gataagctga aaatactcct taattggtca
     1021 tgcggttgcg gaccttatcg accgtgacca gcaggttcgt gagacgatcc ttggtcaact
     1081 catccgccga tttgaccgcg ttaaacgccg cctgcaattc ctggaggtct ttcagtagtt
     1141 tgccgctgtc cggatccttt tcaaactcct tgaacaggtt gaccagattg tccaggtaca
     1201 tccgctgatt gaagatcttt tcaaagatcg cagcgtttcc gggagtgcgc tcctggtcct
     1261 tcatgagcgc gatgctcagg tgttgaactt cgatgatcgc gccggcaagc acgtgggcag
     1321 ccgtcatgta ccggccgttg ttgcgcagat aattatcggt ctccgcatag gctttatcga
     1381 tcatccgaac cagggaatcc tggttgtcgg agttcttctg aaagctctcg gtataccggt
     1441 cgaaggtttc ctgaacattc atcttttcgg ccagggcttt tgtcgtagaa taataggaaa
     1501 gcaagtcctg gttctgacca taaaaggcgg cataagccat gtcgatgccg tagatgccgt
     1561 agttcaccga ttttttatag gcgctggtgt atttctcctc atggtcggtc gaattgagca
     1621 aatcgctgtt gaatggcacc tcattcttgt agatatcgtt gatcaactcc atgggagaag
     1681 gcaaattcgc cagggtgaag aagaatttga aatcaacgag ttccttctgc tccttggaca
     1741 tcgtatcctc aatggtcggc aaggtgtcct gcgaactggc agaatcggtc gaagtctgct
     1801 tacaggcggt ggcggcactc agaaggaccg aggctacaag caagactcgt ttgtggagtt
     1861 tcatacgaga gacgggtttc ctgcaaaaat aggaatagag ggcggaaatg aaagtaaaat
     1921 gttaatccca ttctgcgccg aatcagctca gactcggggc ggtaggcgat tccgggtgag
     1981 caccggcgcc gggaacttac aggattccgg cgccggcaga tgcgttacgg cgcatctcct
     2041 ggatggttag tggctggagg tcattgttct tggtccaaat cacggtgtaa acgccgggtg
     2101 aatgaaccct gcctgaacaa ctggcaggtt tcccggatat ccggcggaag gctgagcagt
     2161 tgcttcacac aacctgataa aaacatgaca atcatccggg agttttttcg ccaatttgta
     2221 cagaagcaac gggccagatt caccgaccaa taacttccgt ataccgctca ttcaatttca
     2281 ttcgcccgaa tttcattttg acattaaaat gtcagggtag attaggcgtt cttccaccat
     2341 tgcttcactg atcggtttac atccccggct actcaagtcc tcactgccct acctatgcaa
     2401 ttgctattga aacccagagt ccctctactt tgggtgtttc tcctgctgct ggtggcatca
     2461 ggggcttcct attatgtagc caatcggggt aagacaaccg ttaagagtga agtcgttccg
     2521 gtacctgccg gtaacgatgt caaaatgaac cggaataccg catttcggtt catccgtccg
     2581 ctgcttttta cggattatag cctggaggat gagaaactga tgccgctgaa aggcgatatc
     2641 atccgctata tcgaaggcca gaagagttcc gggaatatcc aatccgcttc cgtttacctg
     2701 cgcaggttga attcggcctc ctggatgacc attaatccga aagaggaata tccggtcgcc
     2761 agccttttta aagtacccgt catgattgcc atcctgatga cggctgatca ggacccgagt
     2821 ttcctgaata ggaagatctt ttacccggag ttcccggcag gcgtatttca gcacaatgtc
     2881 gctccactgc caacgaataa acactatacg atccggcaac ttctggaaag catggtggct
     2941 cgttccgaca acgaagccta tgacctgctc tggaaagcga tggatcagcg caacttcaac
     3001 aagatgctgt tcgacttcca actcaagtcg tttcagatcc aggatactac cgattacagc
     3061 ctgagtgtgg tagactactc ccgattcctg cgtgtacttt acaatggcgg ttatctaagt
     3121 catgaacgaa gtgagatcgc gctcgacatc ctctccaaat gcgattataa ggaaggactg
     3181 atccgtgatc ttccggctga tctgatctcc tcccgaaaat tcggttatcg tgtcaagcct
     3241 gatcttgctg ctctcagcga gttcggagtt ttttacctca aaggtgagcc gtatctcctt
     3301 ggagtcatga ccaagggtct taattacaac catcttaccg agaccctggc gcatatttcg
     3361 aagctggtat ataactccca gttacccaac agctgagccg gcaccggatt tcaacagctg
     3421 tttcctgcac tccatcctga ttctcctaca tttccgcaag gagatcgtgg caacctgccc
     3481 gattgccagt aacttggcat gtacgctgca aggccatgcc ccggatcctg ctactta
//
LOCUS       DAOTON010000210         3529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_20994_length_3529_cov_3.876223, whole genome shotgun sequence.
ACCESSION   DAOTON010000210 DAOTON010000000
VERSION     DAOTON010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20994_length_3529_cov_3.876223"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="P5292_05305"
     CDS             <1..836
                     /locus_tag="P5292_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HRS38580.1"
                     /translation="GQGVYYGIREAMSWPEDMKKLGLSTGIEGKRVIVQGLGNVGFYS
                     AKFISQGGGIVVAIAEREGGIYNENGLDIEAVFKHRKETGSILNFPGAKNVENTMQTL
                     ELDCDVLVPAALENQITAENAPRIKAKVIAEGANGPVTKEAEEILTQRGVMIIPDLYL
                     NAGGVTVSYFEWLKNLSHVRFGRMEKRFQEMTNLQMLSSIEKITGKYLDARERSLLTR
                     GADEIDLVRSGLEETMIFAYNEIREIRNRTSGISDLRTAAFVSGINKLGVSYQSLGIF
                     P"
     gene            complement(974..2023)
                     /gene="queA"
                     /locus_tag="P5292_05310"
     CDS             complement(974..2023)
                     /gene="queA"
                     /locus_tag="P5292_05310"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRS38581.1"
                     /translation="MKLSQFKISVPDNLIATHPAKNREDARLMVLDRKTGKIEHKKFK
                     DILNYFDDGDCMIVNNTRVFPARLYGNKEKTGAKIEVFLLRELNRESRLWDVLVDPAR
                     KIRIGNKLYFGDDDLLVAEVIDNTTSRGRTLRFLFDGPYEEFKKTVLSLGQTPLPKYI
                     KRKAEPEDADRYQTVFARHEGAVAAPTAGLHFSMPLVKRLELKGVHFAEMTLHVGLGT
                     FRPVEVEDLTKHKMDSEQVIVPKDCCDIVNKAKTAKKRICAVGTTVMRGVETSVSTQG
                     ELKPFDGWTNKFIFPPYDFSIANCMITNFHMPESTLLMMVAAFGGYEHIMHAYKVAVK
                     EKYRFYSYGDAMLII"
     gene            complement(2153..2884)
                     /gene="truB"
                     /locus_tag="P5292_05315"
     CDS             complement(2153..2884)
                     /gene="truB"
                     /locus_tag="P5292_05315"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRS38582.1"
                     /translation="MYSPETDLLTGRVLLIDKPEGWTSFDAVNKVKRALLHAALQGVE
                     QAEERKRIKKKLKIGHAGTLDPLATGLLVVCTGPKTKTISEIQDAEKEYTGTILVGAT
                     TPSYDRELPPDRHYPTDHIRESDIREATRSLTGMQEQLPPAFSALKVGGKRAYELARQ
                     GKEVEMKPRTVEILEFTITDNRFPELDFRVRCSKGTYIRTLAHDLGKRLGSGAYLLAL
                     RRTRIGNYRVEDARTPEAIAAQFAE"
     gene            complement(2895..>3529)
                     /locus_tag="P5292_05320"
     CDS             complement(2895..>3529)
                     /locus_tag="P5292_05320"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRS38583.1"
                     /translation="KKFLPQGSDWAGMIRFYRRLIIAFIPAVIFGLLLKKYIDQLLEN
                     VIVVAVALLVGGIIFLFLDKWFKAAEERGEPELTNDGKAFRIGFFQVLAMVPGVSRSA
                     ATIIGGLTQGLNKKAAAEFSFFLAVPTMFAATVKDLWDFHHEGGGFTGDQLPVLVVGN
                     LVAFVVAVLAIRSFIGYLTKHGFRVFGYYRILVGLAILVLHFLGIDLQMF"
BASE COUNT          782 a         1040 c          929 g          778 t
ORIGIN      
        1 ccggacaggg tgtctattat ggaatccgcg aagcgatgag ctggccggaa gacatgaaga
       61 agctcggact gtctaccggc atcgaaggta agcgtgtcat cgtgcagggt ctcggcaacg
      121 taggcttcta ctcggctaag ttcatcagcc agggcggagg catcgtggtc gcgatcgccg
      181 aacgtgaagg cggtatctac aacgagaacg gcctcgacat cgaagcggta ttcaagcacc
      241 gtaaagagac gggttccatc ctcaacttcc ccggtgcgaa gaatgtcgag aacaccatgc
      301 agacccttga acttgattgc gacgtgctcg ttcccgctgc gctggaaaac cagatcaccg
      361 ctgagaacgc tccccgcatc aaggccaagg tgattgccga aggcgccaac ggcccggtca
      421 ccaaagaagc cgaagagatc ctgacccagc gtggcgtgat gatcatcccc gacctttacc
      481 tcaacgccgg cggcgtgacg gtttcgtact tcgaatggct gaaaaacctt tcgcacgtcc
      541 gcttcggtcg catggaaaag cgcttccagg aaatgacgaa cctgcaaatg ctcagttcga
      601 tcgaaaagat caccggtaaa tacctcgacg cacgtgaacg tagcttgctc acccgcggcg
      661 ccgacgagat cgacctggtc cgctcgggcc tcgaagaaac gatgatcttc gcctacaacg
      721 aaatccgcga gatccggaac cgcaccagcg gcatctccga cctccgcacc gcggcattcg
      781 tcagcggcat caataaactc ggggtatcct accagtcact cgggatcttc ccgtaatcaa
      841 gtaacaccca aacaaaaaag ccccgcacag cggggctttt ttgttgttga aagtttccgg
      901 tttcttgtta ccggtttcga gttatatcaa tttctcgtcc ctcgtccctc gcccctcgtc
      961 cctcgtccct tcctcaaata atcagcatcg catccccata actataaaac cgatacttct
     1021 ccttcaccgc cactttgtag gcgtgcatga tgtgctcata acctccaaag gccgctacca
     1081 tcatcaagag ggtggattcc ggcatgtgga agttcgtgat catgcagttg gcgatcgaga
     1141 aatcgtaggg agggaaaatg aacttgttcg tccatccgtc gaagggcttc aactcgccct
     1201 gcgtggatac gctggtctca acgccgcgca tcacggtcgt gccgactgcg caaatacgtt
     1261 tcttggccgt cttcgccttg ttgacgatat cgcaacagtc tttcggaacg atcacctgct
     1321 ccgaatccat cttgtgcttg gtaaggtctt ccacctcgac cggacggaag gtgcccaggc
     1381 cgacgtgtaa ggtcatttcg gcgaagtgta cgcctttcag ctcaaggcgc tttaccagcg
     1441 gcatgctgaa gtgcaggccg gctgtaggag cggcaacggc tccttcgtgc cgtgcgaaca
     1501 cggtctggta acggtcggca tcttccggct ccgccttgcg cttgatgtac ttcggcagcg
     1561 gggtttgtcc gagcgacaag accgtcttct tgaactcctc gtacgggccg tcgaacagga
     1621 aacgcagggt gcgtccgcgc gacgtggtat tgtcgatcac ttccgcgacc aacagatcgt
     1681 catcgccgaa gtacaacttg ttgccgatcc ggattttccg ggccggatcc accagcacat
     1741 cccacaaacg actttcacgg ttgagctcgc ggagcaggaa gacttcgatc ttcgctccgg
     1801 tcttttcctt attaccatac aaacgggccg gaaacacgcg cgtgttgttc acgatcatgc
     1861 aatccccgtc gtcgaaataa ttcaggatgt ccttgaattt cttgtgctcg attttcccgg
     1921 tcttccggtc caacaccatc aaccgtgcgt cttccctgtt ttttgccgga tgtgtggcga
     1981 tcaggttatc aggcacggaa atcttaaatt gactcagctt catctttgga gcagtagact
     2041 tttacaaacc ctctgaaaat caagagggcg gcaaagatac aatcgtgcta ccccccttgt
     2101 aaagggtttg ccgataaaaa tttgtcgtgt gcaaaatggg gttaatgatt tgctattcag
     2161 caaattgagc tgcgatcgcc tccggtgtcc gcgcatcttc tacccggtaa ttcccaatac
     2221 gggtccgtct cagggcgagc aggtaggcac ccgagccgag ccgctttccg aggtcatgcg
     2281 ccagggtccg gatgtaggtg cccttgctgc aacggacgcg gaagtcgagt tccggaaacc
     2341 gattgtcagt gatcgtaaac tccaggatct cgacggtcct cggtttcatc tccacttcct
     2401 tcccctgtcg cgcgagttca taggcgcgtt taccaccgac cttcagcgcg gaaaaggctg
     2461 gcggcaattg ttcctgcatt cccgtcagcg aacgggtcgc ttcccgtata tccgattcgc
     2521 ggatgtgatc ggtcgggtag tgccggtccg gaggtaactc gcggtcatac gaaggtgtcg
     2581 tcgcaccgac caaaatggtt ccggtgtatt ctttttccgc atcctgaatt tcggaaatgg
     2641 tcttggtctt cggaccggta cacacgacca acaatccggt cgccaacggg tcgagcgttc
     2701 cggcatgacc gatcttgagt tttttcttga tgcgcttccg ttcctctgct tgttccacac
     2761 cctgcaatgc cgcatgcagc aaggctcgct tcactttgtt cacggcatcg aacgaggtcc
     2821 agccttcggg tttgtcgatc agtaaaaccc gccctgtcag caggtccgtt tcgggagaat
     2881 acatgtccgc gcgatcagaa catttgcagg tcgatgccca gaaaatgcag caccaggatc
     2941 gccagtccga ccagaatccg gtagtaacca aacacccgga agccgtgctt cgtcaggtag
     3001 ccgatgaacg atcggatcgc caggacggca acgacgaatg ctaccaggtt gccaacgacc
     3061 agcaccggca actgatcgcc ggtgaatccg ccaccctcgt ggtgaaagtc ccacaggtcc
     3121 ttgaccgtgg cggcgaacat ggtcggcacg gcgaggaaga aggaaaattc cgccgccgct
     3181 tttttattca gaccctgggt gagtccgcca atgatggtag cggccgaacg cgaaactccc
     3241 ggaaccatcg ccagcacctg gaaaaagccg atgcggaacg ccttaccgtc gttggtcagc
     3301 tcgggctcgc cgcgttcttc cgcggctttg aaccacttgt cgagaaagag gaagatgata
     3361 ccgcccacca gcaaggcgac ggcgacaacg atcacgttct ccaacaactg atcgatgtat
     3421 tttttcaaga gcaaaccgaa gatcaccgcg ggaatgaacg cgatgatcag cctgcggtaa
     3481 aaccggatca tccccgccca gtcactgccc tgcggcagga actttttcc
//
LOCUS       DAOTON010000211         3524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21036_length_3524_cov_3.852695, whole genome shotgun sequence.
ACCESSION   DAOTON010000211 DAOTON010000000
VERSION     DAOTON010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21036_length_3524_cov_3.852695"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="P5292_05325"
     CDS             <1..200
                     /locus_tag="P5292_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38584.1"
                     /translation="GLALDNWKFLRPVYPDQPIRCRLVISEVIEHPEKKSRSIRWNFN
                     FEQADGSLVQHLEMLVLHRDS"
     gene            257..814
                     /locus_tag="P5292_05330"
     CDS             257..814
                     /locus_tag="P5292_05330"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HRS38585.1"
                     /translation="MFVSPALVFRPRLILRLVLVWIAVYLIPSYLQAQESGEQVQWYT
                     WQEASKLQQEKPKKLLLDIYTNWCGWCKKMDASTYRDKAVAKYLNEHFYAVKMDAETK
                     DSLHFQGKTYGFKPEYRSNELAAVLLNGQMSYPTTVFLDDKMNPIGPVPGYMDNRQML
                     IVMRYFAEDLYKTKRWEDYLQDELK"
     gene            937..1012
                     /locus_tag="P5292_05335"
     tRNA            937..1012
                     /locus_tag="P5292_05335"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:972..974,aa:His,seq:gtg)
     gene            1108..3303
                     /locus_tag="P5292_05340"
     CDS             1108..3303
                     /locus_tag="P5292_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRS38586.1"
                     /translation="MQEVTDHTQLRVEGMDCANCAQTITRSLRKSGFQDVFVDFATGE
                     VTFEQVGPEKVDAAVDAIHDLGYKVTSRSDQRTTQDDSLESHGLETVTDLSRKFWFCF
                     AFTLPLFLHMFLPFHWLHSPWVQFGLATPVMLIGWMHFGRSAWKSILAGFPNMDVLIT
                     LGASSAYFYSLAGLIRFSGSAAAQEFLFFETAATIITLIFLGNLIEHRSVARTTSAIR
                     ELNRLQPERALRVVTGASGAVQLEPVLVSELRIGDKVQVNEGDRVPVDGRIVYGSGSL
                     DESFLTGESLPVDRTINDSVTGGSIVVKGNFQMIAEHVGTSTTLSKIIAMVKQAQRDK
                     PAIQRLGDRVSAIFVPAVVAIAIITFLVSWLWLQIGVGASVLHSVAVLVISCPCAMGL
                     ATPTAVMVGIGRAARNGILIKGGSTLETLAGIRTVVFDKTGTLTTGNFRIEKINVLQG
                     SLQELKEVLHALESRSSHPIGRSIVRALEQEGFRSGNTLEWERIEEDKGIGINGWTRQ
                     GDLYSIGSFRMVQHFHADLGHSIYVLKNNQLLATADLSDEIKTGAHETVQTLKRKGVR
                     VVLVSGDKADACKLVAASLGIEEVFAEQHPREKLEIIGKLSAEAPTAMVGDGINDAPA
                     LAKAGVGISLSDATQVAIQSSQVILLRSDDLRNLLDALEVSRLTYRTIRQNLFWAFLY
                     NVVAIPIAAAGYLSPMLGALSMAFSDVIVVGNSLRLRTRKIHRPGSRSL"
BASE COUNT          791 a          930 c          956 g          847 t
ORIGIN      
        1 gcggactggc gctggacaat tggaaattcc tgcgtcctgt ttaccccgac cagccgatcc
       61 gttgccgact ggtcatcagc gaagtcattg aacatccgga gaaaaaatca cgctcgatac
      121 gctggaattt caactttgag caggctgatg gcagcctcgt acaacacctt gaaatgctcg
      181 tcctgcaccg ggatagctag attgtaggaa aagctcgaat ttggattttt accctgatcc
      241 gattatattt aacggaatgt ttgtcagccc cgcactcgtt tttcgtcctc gcctgatcct
      301 tcgtcttgtt ttggtttgga tcgccgttta cctgattcca agctatttgc aagcccagga
      361 atccggtgaa caagtgcaat ggtatacctg gcaagaggcc agtaaactgc aacaggaaaa
      421 acccaaaaag ctcctgcttg atatttatac gaattggtgc ggatggtgta aaaaaatgga
      481 cgccagcacc tatcgggata aggcagtggc aaagtatctc aacgaacatt tctacgcggt
      541 taaaatggat gcagagacga aagattcgct gcattttcag gggaaaacat atggcttcaa
      601 acctgaatac cgatcgaacg aacttgctgc cgtcctgctg aacggacaga tgtcgtatcc
      661 taccaccgta tttctcgatg acaagatgaa tccgatcggc cctgtacccg gttacatgga
      721 caaccggcag atgctgatcg tgatgcgtta tttcgcggag gatctttaca agaccaagcg
      781 ctgggaggat tatctgcagg atgaactgaa gtgagcttcc ctttagtgga ggtcaggccc
      841 ggattccgtt tcgcattaac ggcaatgggc gtctttgttt gataatccta aaaatttcct
      901 atttttgcgg ccccgcttgc ccgtcaggcg gttcatggtg attgtagctc agttggttag
      961 agcgccagat tgtggctctg gaggccgcgg gttcgagtcc cgtctttcac ccaaaacagt
     1021 ggctgtcgat gagacagccc tttttgtttt ctgcccattt cgcccggcac tgggtagagg
     1081 ttccgtacct ttgaacgtat tccggccatg caggaagtaa cggatcatac gcaactccgt
     1141 gtagaaggaa tggactgcgc gaactgtgcg caaaccatta cccgatcact cagaaaatcc
     1201 gggtttcagg acgtgttcgt cgacttcgcc accggcgagg tgacctttga acaagtcggc
     1261 ccagaaaagg tcgatgccgc ggtcgacgcg atccacgacc tcggttacaa agttacttcc
     1321 cgttcggatc agcgaaccac acaggatgac tccctggagt cgcacggcct ggagaccgtg
     1381 accgatctga gccgaaagtt ctggttctgc ttcgcattca cgcttccgct tttcctgcac
     1441 atgttcctgc cctttcattg gctgcattca ccctgggttc agttcggtct tgccacaccg
     1501 gtcatgttga tcggctggat gcactttggc cgttccgcct ggaaatccat cctcgcagga
     1561 ttcccaaaca tggatgtcct gatcacactg ggcgccagct cggcctactt ctattcgttg
     1621 gcgggtttga tccgtttctc tggttcggca gccgcgcaag aatttttatt ctttgaaacg
     1681 gccgccacca tcatcaccct gatcttcctt ggcaacctga tcgagcaccg ttcggttgca
     1741 cgaacaacca gtgccatccg tgaactcaac cgtttgcaac cggaacgggc gcttcgcgtt
     1801 gtgaccggtg catcgggagc agtacaactc gagccggtat tggtgagtga actacggatt
     1861 ggcgataagg ttcaggtcaa cgaaggggat cgggtaccgg tggatggtcg aattgtgtat
     1921 ggttcgggtt cactggacga gtcctttctt accggcgaga gcctgccggt ggaccggacg
     1981 atcaatgatt cggtcacggg tggttccata gtcgtgaaag gcaatttcca gatgatcgcg
     2041 gaacatgtcg ggacttccac caccttgtcg aagatcatcg ccatggtgaa acaagcccaa
     2101 cgcgacaaac ccgccatcca acgccttggg gatcgtgtca gtgcgatctt cgttccggcg
     2161 gtggtagcca tcgcgatcat tacattttta gtttcgtggc tatggttgca gatcggcgtc
     2221 ggggcttcgg tgctgcacag tgttgccgta ttggtcatct cctgcccttg cgctatgggc
     2281 ctggctacac cgaccgctgt gatggtcggc atcggacggg cagcacgtaa tggtatcctg
     2341 atcaaaggcg gatcgaccct ggaaaccctg gccgggatcc gcacggttgt cttcgacaag
     2401 acgggcacgc tgacgaccgg caacttccgg atcgaaaaga taaacgtgct gcagggaagt
     2461 ttacaggaat taaaggaagt actccatgcc ctggaatctc gcagttccca cccgatcggg
     2521 cgttcgatcg ttcgggcgtt ggagcaggaa ggattccgat ccggcaacac ccttgaatgg
     2581 gaacgaatcg aagaggacaa aggcatcggt atcaacggct ggacacggca gggtgacctc
     2641 tactcgatcg gatcgttccg gatggtgcag cattttcatg ctgatctcgg ccacagcatc
     2701 tatgtcctga agaacaatca actgctcgca accgctgacc tctccgacga gataaaaacc
     2761 ggcgcccatg aaaccgtcca aacgcttaag aggaagggcg ttcgggtagt tctggtcagt
     2821 ggcgacaaag ctgacgcttg caagcttgtt gcagcatcct taggcattga agaagttttt
     2881 gccgagcaac acccccggga aaagctcgag atcatcggga aactttccgc cgaagcgcca
     2941 acggcgatgg tgggcgacgg catcaacgat gcacctgcac ttgcaaaagc cggtgtcggg
     3001 atcagtttgt cggatgccac gcaggtcgcg atccaatcct ctcaagtcat tttgctgcgt
     3061 tccgatgatc tccggaacct gctggacgcg cttgaagtca gccgactcac ctaccggacg
     3121 atccgacaaa atctcttttg ggcattcctc tacaatgtgg ttgcgatacc gattgcagcc
     3181 gcgggttatc ttagcccgat gttgggcgct ttatcgatgg cattttccga tgtgatcgtt
     3241 gtcgggaact ccctgcgtct gcgtacccga aagatccaca ggcccgggag ccgaagccta
     3301 tgaccccgca tcctcctctt aaacatattc tgtggatctg caggtggtat tccaaccgtg
     3361 aggatcccca gttgggtgtt ttcatccaaa agcaggctga agcgttggcg gatcattacc
     3421 gggtgtgtgt ggtttacgcg atggcggtgg atcaaggtga gacaggatct gtaatacggg
     3481 aaaacgggaa cttactcgag atcattcagt attaccccag acgc
//
LOCUS       DAOTON010000212         3516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21109_length_3516_cov_2.807281, whole genome shotgun sequence.
ACCESSION   DAOTON010000212 DAOTON010000000
VERSION     DAOTON010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21109_length_3516_cov_2.807281"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1994
                     /locus_tag="P5292_05345"
     CDS             <1..1994
                     /locus_tag="P5292_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38587.1"
                     /translation="GSLAISGGQLNVKGGTGTGVVDINGNYTHSGGWFQLHNNSGAGT
                     NDAVRVTVAGAFNHSSGTIQFENYGMPTGVIHRLILKGSTADFSGNGIMTSASPGTGS
                     DFGVLSFERTGTITYRKTGNVYSIQQVLQEIATGCTLRLSAGPLLISSQMETGVYALT
                     IGVNATLDAGNQQISSNGLQPYASVFLDSAAVLRTSKSAGLYDHGNNGTLDATNNLSF
                     KLHPYSIVEYNGSSSQDLTGYSASRTLTTDQYYGILRIGLTSGASARAKYDFIPVRTR
                     LELVSGSIDLDGKELQLMSGDSQAITRTAGGIISDDHSGSGSGRVVMKGIQSGPHLFP
                     FIDENGSYLPVILQPTSGFGADVTISSNHTAQDNRPFPSGLSTVAVTTDLIAGLSTDN
                     LIDRWWEITASGLTANVSFSYSALENTLPIDLRLSYLGVVRWTGSGWTQPAGSGLGVT
                     LGTGMVTMSNVSTFGIFGLSANNLALPIELVSFEAKAKATEVEITWTTAAEVNNDYFT
                     IERSKDGIEFEAIQQIDGAGNSTNILHYGVVDKTPKSGTSYYRLRQTDFDGRFTYSEI
                     RAVNVTGDPPGPGIRIEQAGPNPFNELFRVFYAVNDATEIQVMLIDPAGKPVLNDKVL
                     ATPGSNTYEFRYGSGLPSGTYIFHLTDGTERSSVKLIKK"
     gene            2174..3439
                     /locus_tag="P5292_05350"
     CDS             2174..3439
                     /locus_tag="P5292_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38588.1"
                     /translation="MLITAGWLVYLNFHNLSKTRASGEGESQGGAVLNNGEIITEFTW
                     EKDSPLEATLGPDAISASEDAHTFRGGRASTQGLAAGSNGNDINLELPGTELFDVEGI
                     DVSIDYRGTEPEGSFLSRGNHFNFGISDGYLSIQYRVDNRKGGFTSVKEKTTYELPKD
                     ENFRTYRFIYSPTTGRGEIFVNGMPVWSKGGLANTVMYWKGAGNLYVGRGMNGGGKDM
                     AILDNLVIRTTGTISPLAESLLNFSLEGKENGVNVHWLTAVNDQVAYFTVERSINGLD
                     FSKVGTINVNPKAGELGEYNYLDKTPIGETVAYYRIRQTFKNGKFVSHSLAAIRTKAS
                     RDFSIERVSPVPFNDNFEVSYFMPNSGRVWLQLTDPKGKILSTETFEAPQGKNIHSFR
                     DKNNLPPGNYVLHMIYDNKKVSANITKSM"
BASE COUNT          934 a          943 c          888 g          751 t
ORIGIN      
        1 ttggaagcct tgccatcagc ggaggacaat tgaatgtcaa aggagggacc ggaaccggag
       61 tggtcgacat caacggaaac tacacgcatt ccggcgggtg gttccagcta cataacaatt
      121 ccggtgcagg aaccaacgat gccgtacgcg taaccgtagc cggcgcattc aatcactcct
      181 ccggcaccat acagtttgag aattacggta tgccgaccgg cgtcattcac cggctcatac
      241 tcaaaggatc aacggcggac ttttccggga atggaatcat gacgagcgct tcaccgggaa
      301 cgggcagcga cttcggagta ttatcatttg aacgaaccgg cacgatcact taccggaaga
      361 ccggaaatgt gtactccatt cagcaagttc tgcaggaaat cgcaaccggc tgcactctcc
      421 gattatctgc aggccctctc cttatcagca gccagatgga aaccggcgtc tatgccttga
      481 caatcggggt gaatgcgacc ctggatgcag gcaaccaaca gatcagcagc aacgggctcc
      541 agccctacgc ttcggtattt cttgattcag ctgccgtcct gcgaacatcg aaatcggccg
      601 ggctgtacga ccacggcaat aatggtacgc tggacgcgac caataatctg agtttcaagt
      661 tgcatccgta tagtatagtc gaatacaatg gcagctcctc acaagacctt acagggtatt
      721 ctgcttcgcg cacattaact accgatcaat attacggcat attgcgaatc gggctcacct
      781 ccggcgcttc cgccagggcg aagtacgact tcattccggt cagaacacgc ctggaactcg
      841 tgagcggatc cattgatctg gatggcaagg aacttcaatt gatgagtgga gacagccagg
      901 ccatcacacg aaccgcaggc ggaatcatct ccgacgacca ctccggcagc ggttccggcc
      961 gggttgtgat gaaaggcatt caatcggggc ctcatctctt tccattcatc gatgaaaacg
     1021 ggagctactt accggttatc ctacaaccaa cgtccggctt cggcgcggat gtgacgatct
     1081 cttccaatca tacggcacag gataaccggc cattccccag tggactctct accgtggccg
     1141 tcacaaccga tttgattgcc ggcctatcga ccgataatct catcgaccgg tggtgggaga
     1201 tcactgccag tggtctgacg gcgaatgtct ccttctccta ctccgcactt gagaacacgt
     1261 taccgattga tctccgacta tcgtatttag gcgtcgttcg ctggaccggt agcggttgga
     1321 cccaaccggc gggaagcgga ttgggcgtga cattgggtac cggtatggtg accatgagca
     1381 atgtcagcac cttcgggata ttcggattat cggcgaacaa cctggcattg cccattgaac
     1441 tggtctcctt cgaggcaaag gcgaaagcta cggaagtaga aatcacctgg acaacagcag
     1501 ccgaagtgaa caacgactac ttcaccatcg aacgcagcaa ggacggcatc gagttcgaag
     1561 cgattcaaca aattgatggt gcaggaaaca gtaccaatat tctgcattat ggagtggtcg
     1621 acaaaacgcc gaagtccggc acctcctatt accgtctacg gcagacagat tttgatggcc
     1681 gattcactta ctcggagatc cgggcagtca acgtgaccgg agatcctccg gggccgggca
     1741 tccgcattga acaagccgga ccgaatccgt ttaatgaact cttccgggta ttctacgcag
     1801 tcaacgatgc aaccgaaata caagtcatgc tcattgaccc cgccggaaaa ccggtgctga
     1861 atgataaagt ccttgccact ccaggctcaa acacctatga gttccgttat ggttcaggct
     1921 tgcccagtgg cacctatatt ttccacctga ccgatggaac tgaaagaagc agcgtaaaac
     1981 tcattaaaaa ataaatgtga ctgtaaggaa tcatccggct tttcactcct taagaatgcg
     2041 atcactgtag tttatatacg tcccgccata ccctaacccg aacgcaatga agaataagag
     2101 gaatacacga cttggcaaca gaacaagatt atccacccga ttgatttggg ttgttggtac
     2161 tttttctgct gtcttgctga taacggccgg gtggctggtt tacctgaact tccacaacct
     2221 aagcaagacg cgagcgagcg gagaaggtga aagtcagggt ggggccgtgt tgaacaacgg
     2281 ggagatcatc actgaattta cctgggaaaa agacagtcca ctggaggcta cccttggccc
     2341 cgacgcaatc tccgccagtg aggacgccca taccttccgc ggtggacgtg cttccacaca
     2401 aggtctcgca gccggttcca atggaaacga tatcaacctc gaacttccag gcactgagct
     2461 cttcgatgta gaagggatcg atgtcagcat tgactatcgt gggacggagc cggaaggcag
     2521 ctttctgagc cgagggaatc atttcaactt cggcatctcg gatggctact tatccattca
     2581 ataccgggtc gacaaccgca agggtggttt cacgtccgtt aaggagaaga cgacctacga
     2641 acttccgaaa gacgaaaact tccgaaccta ccggttcatc tactcgccca ctacaggccg
     2701 cggcgagatc ttcgtaaacg ggatgccggt ctggtcaaaa ggggggctgg cgaataccgt
     2761 gatgtattgg aaaggagcag gaaacctgta tgttggtcgc ggaatgaatg gcggcggaaa
     2821 ggacatggcg atcctggaca acctggttat tcggacaaca ggtaccatat cgccacttgc
     2881 agagtcactc cttaatttct ccctcgaagg caaagagaac ggcgtcaacg tacattggct
     2941 gacggctgtc aacgaccagg tcgcctattt taccgtagag cgatcgatca atggtctcga
     3001 cttttccaaa gtcggaacga tcaacgtaaa tccgaaggcg ggagagttgg gtgaatacaa
     3061 ttatcttgac aaaacaccga ttggcgaaac ggtcgcatac tatcgcattc ggcagacctt
     3121 caagaacgga aaattcgtca gccattcact ggcagccatt cgcacgaaag ccagtcgcga
     3181 tttcagcatc gaacgggtca gtcccgtccc cttcaatgac aacttcgagg tttcctactt
     3241 catgccgaac tccggtcgcg tctggttaca actgaccgat ccaaagggaa aaatcctttc
     3301 aaccgaaaca tttgaagctc cacagggcaa gaacattcac agtttccggg acaagaacaa
     3361 cctgccaccc ggcaactacg tcctgcacat gatctatgac aacaaaaagg tcagcgccaa
     3421 catcaccaag agtatgtaac gagtaaacga cgataaaaaa gcgaagggct actgtcacca
     3481 gtagcccttc gcttttttaa tgaacgggtg aattta
//
LOCUS       DAOTON010000213         3514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21124_length_3514_cov_3.421509, whole genome shotgun sequence.
ACCESSION   DAOTON010000213 DAOTON010000000
VERSION     DAOTON010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21124_length_3514_cov_3.421509"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="P5292_05355"
     CDS             complement(<1..462)
                     /locus_tag="P5292_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38589.1"
                     /translation="MISKVRAFFILLISGILFSACKKPATEMVFSNDFEELGNWYWVP
                     TLQIGEGHSGFHYSATDSLNPYSITFSRTIFELGPQQMNGAEYSAWVRVREAGGFFNL
                     VCTVEKEGKNTVHCSQDFRPESGEIGQWVHYQLICDFPPGLDRDSRIKLYVF"
     gene            542..1489
                     /locus_tag="P5292_05360"
     CDS             542..1489
                     /locus_tag="P5292_05360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019395422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS38590.1"
                     /translation="MKTLSVIIPIYNEEGNIPRLYERLNGVVQQLGVDTEFVFINDGS
                     RDRSIELIRGLASRDRNVRFIDFSRNFGHQIAVTAGLDHCTGKAAVIIDADLQDPPEL
                     ILDLFAKWQEGYEVVYAKRRARQGEGFLKKLTARYFYRTLKKITSINIPVDTGDFRII
                     DRKIIEVLKQMPEQQKFLRGQISWIGFRQTYVEYDRDARHSGETGYTYKKMIRFALDG
                     ITSFSNLPLKFATMAGFFVSGVAFLLILYALYARFVTRDYVPGWTSLMLAVLFIGGVQ
                     LISLGVIGEYISRLSANIRNRPLYIVRDSNTESAGGETQ"
     gene            1499..3388
                     /locus_tag="P5292_05365"
     CDS             1499..3388
                     /locus_tag="P5292_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38591.1"
                     /translation="MTLPNNASARNRTSLVALFLVLAGILVGHFTLPRLKHQYYTQEL
                     TFDVTSYYLYLPMTFIYDDPGIRNKATIDTLFARYNPSPTFYQAFELENGNRVMNYTC
                     GFAYAYAPFFFLAHSWATGSNYPADGFSFPYQLLVGLGVYCYILFGWIILRRLLLRYF
                     SDTVTAFVLLFTCFGTNYFSEAVNNYLQPHAMLFTGYAVLLYCISKWHEQPKRKYMVA
                     GGLVMGWMVLSRPSEIVCVLLPLLWGVYDKESLRAKWELVRKNFSHVLILVVCGIVVF
                     IPQFIYWHKVTGSAIFFSYQRTEGFDFLKPHILNNLFSFKKSLFVYTPILIFPLIGLF
                     LIRRYNRYIQWAVVAYAAVNFYLLASWAAWWNGGSFGMRYFAESYAVMTLPFGYVITE
                     LGKRQRWLRLLSGAALSFFVFLNLFQTWQFLHWIIPDDRMTYAYYKRIFLKTKVTDED
                     RKLMEVERSFAATETFDNEQEYDHFTQAFYDFDSLNSGVLDPSRTDTTFAYSGKHSCK
                     LSPEYPYYPTYRIRYDQLVPWNKDHVWLRVSCWYYSKTDIRENPASLVIVMPHKKYNL
                     KYRAFDFEQQPFVPGQWNKITADYMTPRPYSEADKFEIYAWYRGNSEIWIDNFQVEAF
                     VRKDP"
     gene            3385..>3514
                     /locus_tag="P5292_05370"
     CDS             3385..>3514
                     /locus_tag="P5292_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS38592.1"
                     /translation="MSTMSPYTLSIIVPAYNEEKTIRTILEKVHEVTLIAGGKKEIV"
BASE COUNT          812 a          984 c          858 g          860 t
ORIGIN      
        1 aaagacatat aatttgatcc tgctatcgcg gtcaagaccg ggtggaaaat cacagatcaa
       61 ctgatagtgg acccattgtc caatttcgcc ggattccggt cgaaaatcct gagaacagtg
      121 cacggtgttt ttcccttctt tctctaccgt acaaaccaga ttaaagaaac ctccagcctc
      181 ccgaacccga acccaggcgg aatactctgc tccgttcatt tgttgtggcc ccagttcgaa
      241 aatcgtcctt gaaaaggtga tgctataggg attcaggctg tcggtcgctg aataatggaa
      301 acccgaatgt ccttctccga tctgtaaggt cggcacccag taccagttcc ccaattcctc
      361 gaaatcgttg gagaaaacca tctccgtcgc cggtttttta caggccgaaa aaaggatacc
      421 ggatatgagc aggatgaaaa acgctcgaac cttggaaatc atcgtattgc aaaggtaaga
      481 attggtgccg gggaaagggt cgttccgggg ggattttcta tttttcgggc ctgattttcc
      541 catgaaaaca ctatcggtca tcatccccat ttacaatgag gaaggcaata tcccgcggct
      601 gtacgaacgc ctgaacgggg ttgtgcagca attgggagtc gataccgagt ttgttttcat
      661 caacgacggt agccgcgacc gatccatcga gctcatccgg ggattagcga gccgtgaccg
      721 caatgtccgg ttcattgatt tctcccggaa cttcggtcac cagatcgctg tcaccgccgg
      781 actcgatcat tgtaccggta aagccgccgt catcatcgac gccgatctgc aggacccgcc
      841 cgaactgatc ctcgacctct tcgccaaatg gcaggaaggc tacgaggtgg tctatgcgaa
      901 acgtcgcgcg cgccagggcg agggcttttt gaaaaagttg acggcccggt atttctatcg
      961 taccctgaaa aagatcactt ccatcaacat tcccgtcgat accggcgact tccgcatcat
     1021 cgaccgtaag atcatcgagg tgctcaaaca gatgccggag caacagaagt tcctgcgcgg
     1081 gcaaatcagt tggataggtt tccgccagac ctatgtggaa tacgaccggg atgcgcgtca
     1141 ctcgggggag accggttata cctacaagaa aatgatccgc ttcgcgctcg acgggatcac
     1201 ttcgttctcg aacctgcccc tgaagttcgc caccatggcg ggcttcttcg tctccggtgt
     1261 cgcctttctg ctcatcctct atgccttgta cgcgcgtttc gtcacgcgcg actatgttcc
     1321 gggctggact tccctgatgc tggccgtgct gttcatcggc ggagtacagt tgatctcact
     1381 gggtgtgatc ggagagtata tcagtcgcct aagcgccaat atccgcaacc gtccgctcta
     1441 catcgtacgc gattcgaata ccgaatcggc cggcggcgaa acacaataag ctttctccat
     1501 gaccctaccg aacaatgcct ccgccaggaa tcgtacctcg ctcgtagcgc tcttcctggt
     1561 gttggccggc atcctcgtcg gacatttcac gctaccccgg ctcaagcatc agtactatac
     1621 acaggaactc actttcgatg taacctcgta ttacctgtac ctgccgatga ccttcatcta
     1681 cgatgatccc ggcatccgca acaaggcaac catcgatact ctctttgccc gctataaccc
     1741 cagcccgacc ttttaccagg ccttcgaact ggagaacggc aaccgggtga tgaattacac
     1801 ctgtggtttc gcatacgcct acgcgccctt cttcttttta gcgcactcct gggccaccgg
     1861 cagcaactat ccggctgatg gattttcatt cccttatcag ctcctggtcg gactgggcgt
     1921 ctattgctac atactattcg gctggatcat ccttcgaaga ctcctgctgc gctatttcag
     1981 cgacacggta acagccttcg tgctcttgtt cacgtgtttc ggaaccaact acttctccga
     2041 agcggtcaac aattacctcc agccgcacgc gatgctcttc accggttatg cggtactcct
     2101 gtactgcata tcgaaatggc acgaacaacc gaaacgcaag tacatggtcg caggcggtct
     2161 ggtcatgggc tggatggtgc tgtcaagacc cagcgagatc gtttgcgtgt tactaccctt
     2221 gctatggggc gtttacgaca aagagagtct gcgggccaag tgggaattgg tccggaaaaa
     2281 cttctcccat gtgttgatcc tggttgtctg cggaattgtc gtgttcatcc cgcagtttat
     2341 ctactggcac aaggtgacgg gcagtgcgat ctttttcagt tatcagcgga cagaaggatt
     2401 cgactttttg aaaccgcata ttctgaacaa cctgttttcc ttcaagaaaa gtcttttcgt
     2461 ttatacgccg atcctgatct tcccgttgat cggattattc ctgatccgcc gctacaaccg
     2521 ctacatccag tgggccgttg tcgcttacgc agccgtcaat ttttatttgc tggccagttg
     2581 ggcggcctgg tggaacggcg gaagtttcgg gatgcgctac ttcgcggaat cctacgccgt
     2641 gatgacgctt cccttcggct acgtcatcac cgaactgggc aaacgtcaac gctggctacg
     2701 cctgttgtcg ggcgcggcct tgagcttctt tgttttcctg aatcttttcc agacctggca
     2761 gttcctgcac tggatcatcc cggacgaccg gatgacctat gcctactata agcggatttt
     2821 cctgaagacc aaggtcacag acgaagaccg gaaactcatg gaagtggaac gttcgttcgc
     2881 cgcgacggaa accttcgaca acgaacagga gtacgatcat ttcacgcagg cattctacga
     2941 cttcgacagc ctgaactccg gtgtgctgga tccttcacgg accgatacca cattcgctta
     3001 ctccggcaaa cattcgtgta agctgtctcc tgagtatccc tactatccaa cctaccggat
     3061 ccgttacgac caattggtcc cgtggaacaa ggaccatgta tggttgcggg tctcgtgttg
     3121 gtattattcg aaaaccgata tccgggaaaa tcccgcatcg ctggtcatcg tgatgccgca
     3181 taagaagtat aacctcaaat accgggcatt cgacttcgaa cagcaaccct tcgtaccggg
     3241 ccagtggaat aagataacgg ccgattacat gacgccgaga ccgtattcag aagctgataa
     3301 attcgagatc tatgcctggt accgcggcaa ttcagaaatc tggattgaca actttcaggt
     3361 cgaagctttc gtaagaaaag atccatgagc acgatgagtc cttataccct ttccatcatc
     3421 gttcccgcgt acaacgagga gaaaaccatt cgcaccatcc tggaaaaggt gcacgaagtg
     3481 accttgattg ccggcggaaa aaaagagatc gtgg
//
LOCUS       DAOTON010000214         3500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21211_length_3500_cov_3.683309, whole genome shotgun sequence.
ACCESSION   DAOTON010000214 DAOTON010000000
VERSION     DAOTON010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21211_length_3500_cov_3.683309"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1120)
                     /locus_tag="P5292_05375"
     CDS             complement(101..1120)
                     /locus_tag="P5292_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRS38593.1"
                     /translation="MNGSAKPDILLTGSTGLVGARLLLDLRMRGHSVRAFRRPTSSMG
                     VVNRMFRGQESLLEGVDWFEGDLLDLYDINEALSGIRFVFHAAALVSFAPSDRDLLLR
                     VNKDVTADLVNACLELGVEWLGYVSSVAALGRTDEGVPVDENTFWKTSRYNTAYAISK
                     YGGEREVWRGMEEGLNACMVNPTIVLGPGDWNFGSSALFRRVYEGLRLYPLGSTGFVD
                     VRDVSSALLRCWDQRITGERFLLSSENVGYKELLSWMSEGFGKEAPRIPVKPWMAGLA
                     WRWESLFSSITGRKPLVTRETARSSGKQWVYRNDKARKVLGMDFIPVRESVLENCRIF
                     LDSIR"
     gene            1204..2487
                     /gene="tyrS"
                     /locus_tag="P5292_05380"
     CDS             1204..2487
                     /gene="tyrS"
                     /locus_tag="P5292_05380"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRS38594.1"
                     /translation="MKKDFVEELKWRGMLQDMIPGTDELLRKQMVRGYIGFDPTADSL
                     GVGNMVQIMTLLHFQQAGHQPIALIGGATGMVGDPSGKSQERNLLDEAQLQHNLQRQK
                     AQLEKFLDFDCGANSARIVNNYDWFREFSFLDFIRDVGKHITINYMLAKDSVQKRLET
                     GMSFTEFSYQLVQGYDFYHLWKNEGVLLQMGGSDQWGNIVTGTELIRRKSSGEAFALT
                     TPLIKKSDGTKFGKTEGGNVWLDPKRTSPYKFYQFWLNASDSDASNYIRIFTLRSKEE
                     ITAIEEEHAKAPHLRFLQKELSADITARVHSKADLDAAIEASEILFGKGTADALKALS
                     EDDLLAVFEGVPQMRIARTELENGIPVIDLLADKTGIFPSRGETRKMLQGGGVSINKQ
                     KVEDIERVAGKDDLLNNRYLLIQKGKKNYYLIIAD"
     gene            complement(2484..2711)
                     /locus_tag="P5292_05385"
     CDS             complement(2484..2711)
                     /locus_tag="P5292_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38595.1"
                     /translation="MISNRRRIFIVIISVTIGSLLAGWIMQKRMGHLSRENYMSLMFN
                     FIFAAAVVVGLMIFLGRMNKRDQSDDQQDQP"
     gene            complement(2760..>3500)
                     /locus_tag="P5292_05390"
     CDS             complement(2760..>3500)
                     /locus_tag="P5292_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HRS38596.1"
                     /translation="SSGTTGLSGSLHHVLDRKLYERSFLECFRLFYGDPKKYCILALL
                     PSYLERNDSSLVYMTDHLIRESDHPDSGFFLNKFDHLHDSLLRLRERAQPTLLLGVTF
                     ALLDFAERFQLNWPELIVMETGGMKGRRKELVRAEVHQRLCPAFGVSRIHSEYGMTEL
                     LSQAYSSGEGLFRTPPWMRIVIRDPNDPFETRNSGSGGINVIDLANLYSCSFLETGDL
                     GRIAGAGSFEVLGRFDHADIRGCNLLVL"
BASE COUNT          877 a          975 c          917 g          731 t
ORIGIN      
        1 ggatttgctc tctgagcttt acgatgatgt cattgacgaa ctgagcagga tccaagggga
       61 agcacagcag aaaaacgact gacccgccac cggtgtcagt ctagcgtatt gaatccagaa
      121 agatcctgca attttccagt accgattccc tgaccggaat gaaatccatt ccgagcactt
      181 ttcgcgcttt gtcattccgg taaacccact gcttaccgct gctgcgtgcc gtctcccggg
      241 ttaccagtgg ttttcgcccc gtgatgctgg agaaaagcga ttcccaccgc catgccaatc
      301 cggccatcca gggtttaacc gggatacgtg gcgcctcttt gccaaatcct tcactcatcc
      361 acgataacag ttctttatac ccgacgtttt ctgacgaaag cagaaaacgt tcacctgtga
      421 tgcgttgatc ccagcagcgt agtagcgcac tgctgacatc ccgaacgtcg acgaagcccg
      481 tcgatcccaa cgggtagaga cgcaggcctt cgtatacacg cctgaacaaa gcggaacttc
      541 cgaaattcca atcacccggg cccaatacaa tggtcggatt caccatgcag gcgttgagtc
      601 cttcctccat tcctctccaa acctcccgtt cgcctccgta cttgctgatg gcgtatgcgg
      661 tattgtaacg cgatgtcttc cagaatgtgt tttcatcgac cggcacccct tcatccgtcc
      721 tgccaagtgc agctactgag ctgacgtacc ccaaccactc cacgcccaac tcgaggcagg
      781 cattcacgag gtccgccgtc acgtccttgt tgacacgcaa aagcagatca cggtcggatg
      841 gggcaaagga aaccagggcc gcggcatgaa agacgaaacg aatccccgaa agcgcttcgt
      901 tgatatcata taggtcaagc agatcgcctt cgaaccagtc tactccttcc agcagggatt
      961 cctgcccccg gaacatgcgg ttaaccacac ccatggaaga agtcggcctc ctgaaagccc
     1021 ggactgaatg gccacgcata cggagatcca gcagcaggcg agcgcccacc aggccggttg
     1081 acccggtcag caggatatcc ggtttggcgc ttccgttcat ggcctgcgaa gatgggaata
     1141 gttttgaaaa aggagggtag ccataacgta gatgacggct atctttgacg ccaaatccga
     1201 tctatgaaaa aggactttgt cgaggaactg aagtggcggg gcatgttgca ggatatgatc
     1261 cccgggaccg atgaactttt aagaaagcag atggtccgcg ggtatatcgg attcgacccg
     1321 accgctgatt ccctgggtgt cggcaacatg gtgcagatca tgaccttgct gcacttccag
     1381 caagccgggc atcaacccat cgccctcatc ggaggcgcta cgggtatggt cggcgatcct
     1441 tccggcaaat cgcaggaacg caatcttctg gatgaagcac aattgcagca caacctgcag
     1501 cggcagaagg cacaattgga gaaattcctt gatttcgatt gtggtgcgaa ctcagcgcgc
     1561 atcgtcaata attacgactg gttccgcgaa ttctccttcc tcgattttat ccgggatgtg
     1621 ggaaagcaca tcacgatcaa ttatatgctg gcaaaggatt cggttcaaaa gcgccttgag
     1681 accggcatgt cgtttacgga gttctcctac cagttggtcc agggctacga cttctatcac
     1741 ctgtggaaaa acgaaggagt cctgttacaa atgggcggct ccgaccaatg gggcaacatc
     1801 gtgaccggta cggagctgat ccgccgcaag tcatcgggag aagctttcgc gttgaccact
     1861 ccgctgatca agaaatcgga cggaacaaaa ttcggcaaga cggaaggcgg aaatgtctgg
     1921 ttggatccca agcggacctc tccgtacaaa ttctaccagt tctggttgaa cgcttccgat
     1981 tccgacgcat cgaattacat ccgcattttc acgctccgtt ccaaggaaga gataaccgca
     2041 atcgaagagg aacacgccaa ggctccacac cttcggtttt tgcaaaaaga actcagcgcc
     2101 gatattaccg cgcgtgttca ctcaaaggcg gacctcgacg cggctattga agcgtccgag
     2161 atcctgttcg gcaaagggac cgccgacgcc ctgaaagcat tatcggagga cgacctgttg
     2221 gcggtattcg aaggggtgcc acagatgcgg atcgcgcgca cggaactgga aaatggaatt
     2281 cccgtgatcg acctgctggc cgacaagacc gggattttcc cgtcgcgcgg tgaaacgcgc
     2341 aagatgttgc agggtggcgg agtctccatc aacaaacaaa aagtggaaga catcgagcgg
     2401 gtcgccggaa aggatgacct gctgaacaac cgctatctcc tcattcagaa aggcaagaag
     2461 aattactacc tgatcatcgc cgattaaggc tgatcctgct gatcatcgga ttgatcgcgc
     2521 ttgttcattc tccctaagaa aatcatcagc ccaacgacca ctgcggccgc gaaaatgaaa
     2581 ttgaacatga ggctcatgta attctcgcgg ctcaggtgac ccatgcgttt ttgcatgatc
     2641 caaccggcga ggaggctgcc gatggtgacg gaaatgatga caatgaaaat tcggcggcgg
     2701 ttggaaatca tacgctgagt ataggctgca aaggtaggaa gtttccgaga gtagcccggc
     2761 tataacacca ggaggttgca gccacgaata tccgcatggt cgaagcgacc cagcacttca
     2821 aagctgcctg ctccggcaat tcgaccgagg tcgccggtct ccaggaaaga acatgaatac
     2881 aggttggcca ggtcgatcac gttgatgccc ccgctaccgc tgttccttgt ctcgaacgga
     2941 tcattgggat cccgaatcac gatgcgcatc cagggtggcg ttctgaacaa accttccccg
     3001 ctcgagtagg cttgcgacaa caactccgtc atgccgtatt cggaatggat ccgacttaca
     3061 ccgaaggccg ggcacaggcg ttggtgcact tcggctcgta ccagttcttt ccggcggccc
     3121 ttcataccgc ctgtttccat tacaatcagt tcgggccagt tcaactggaa acgctccgcg
     3181 aaatcaagca gggcgaaggt gacaccgagt aaaagcgtgg gttgggcccg ctcacgcaac
     3241 cgcagcaggc tatcgtgcag atggtcgaac ttgtttaaga agaaccctga atcaggatgg
     3301 tcggattccc ggatcaaatg atccgtcatg tagacaaggg aggaatcatt ccgctccagg
     3361 taagacggca acaaggccag gatgcagtac tttttcggat cgccgtaaaa caaccggaag
     3421 cactccaaaa acgatcgctc atagagtttc cgatcaagca cgtgatgcag actacccgac
     3481 agaccagtcg tgccgctgct
//
LOCUS       DAOTON010000215         3497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21229_length_3497_cov_3.161534, whole genome shotgun sequence.
ACCESSION   DAOTON010000215 DAOTON010000000
VERSION     DAOTON010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21229_length_3497_cov_3.161534"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="P5292_05395"
     CDS             complement(<1..128)
                     /locus_tag="P5292_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRS38597.1"
                     /translation="MAEILLEENKERFVLFPIKYPKIWEMYKKAEQSFWTAEEIDL"
     gene            complement(787..1371)
                     /locus_tag="P5292_05400"
     CDS             complement(787..1371)
                     /locus_tag="P5292_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HRS38598.1"
                     /translation="MLAIGDTLVSDEIIEKHFVCDLNACKGACCVKGDYGAPLEEEEL
                     KELEKVYPKVKPYLPASGVKAIEKQGLYLRYNTGEWVTPLVKGKECAYTIFEADGTAK
                     CGIEKAYFDGKISFRKPISCHLYPIRLNKHRSGLEAANYDRWSVCRPACKLGDSLKVP
                     IYKFLKDALIRKYGKAWYKKLELAAKEYEAAKGS"
     gene            1726..3273
                     /locus_tag="P5292_05405"
     CDS             1726..3273
                     /locus_tag="P5292_05405"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRS38599.1"
                     /translation="MASTNQDDLFKNIISHAKEYGFIFQSSEIYDGLSAVYDYGQNGA
                     ELKKNIREYWWKSMVQMNENIVGIDAAIFMHPTTWKASGHVDAFNDPLVDNKDSKKRY
                     RADVLIEDHVAKIEGKIRKEVEKARTRFGDSFSEHQFRTTNARVLEYQQQIDTINQRF
                     KAALEANDLNEVRQIILDLEIVCPISGTRNWTEVRQFNLMFSTQLGSVSEEASTIYLR
                     PETAQGIFVNFLNVQKTGRMKIPFGIAQTGKAFRNEIVARQFIFRMREFEQMEMQYFV
                     KPGTEMEWYNFWKEARMKWHRALGFPDGKHRFHDHLKLAHYANAACDIEFEFPFGFKE
                     LEGIHSRTDFDLGNHEKFSGKKLQYFDPELNQNYVPYVVETSIGLDRMFLAVLSTAYA
                     EEQLEDGSERVVLRIPPFLAPIKCAVLPLVKKDGLPEKAREIMDRLKFDHLCVYEEKD
                     SIGKRYRRMDAIGTPFCITVDHDSIEQNTVTLRDRDSMQQERVSIDRLPEIVGEKVDM
                     RKALRGL"
BASE COUNT          796 a          989 c          968 g          744 t
ORIGIN      
        1 tgcaggtcga tctcttcggc ggtccagaag ctctgctccg ccttcttata catttcccaa
       61 atttttgggt atttgattgg aaagagtacg aaacgctctt tattctcttc taacaggatc
      121 tcggccataa attcaatcta aacggttagg ggtgtgcact atttcggatt aggggagaca
      181 cttcctacgc cctcgggaac ctggttttta aaaaacgccc gtgggaggag ggttatttcc
      241 tttaaagtcg cttgaattcg cacatcgaat cattcccggt gtgcgaaaca aaaattctca
      301 tctctatccg accctgcaat agtcgcccgg gatttttttc gacaattatg caccgcatcg
      361 tgtgaaaaaa tccggtcgac ctcggcaaac ccgctccagt caaggctttc cggaggtatg
      421 aacaaatttt gttcaaaact cctgcttgac aatcatgcag gatttcctaa ctcaggaagc
      481 gctaataagc gtccgaaaaa ttttttgaat tgataatcag ggagacgccg gaaacggcgc
      541 tggaggttgt tgcaaatgtc ggtgagacgg gttgatggtt tcgagtttcg ggtttcgggt
      601 tccgagttag ttgaaagttg aaggttgaaa gttgaaaaag tcgatgcgag aagtagggtg
      661 ttccgggatt cgagttggtt gaaagttgaa ggttgaaagt tgctggccat cctcgttcct
      721 cgcacctcgt ccctcgcccc tcgcccctcg gtcacggtct cgtccctccc ttccaacccg
      781 gaacaattaa ctcccctttg cggcttcgta ctccttcgcc gccagttcca gcttcttgta
      841 ccaggccttt ccgtacttcc ggatgagggc gtctttgagg aacttgtaaa tcggcacttt
      901 gaggctatcg ccgagtttgc aggccgggcg acaaaccgac cagcggtcgt agttggcggc
      961 ctctagtccg ctgcggtgct tgttcaggcg aatcggatac aaatggcagg aaatcggttt
     1021 ccggaaactg atcttgccgt caaaatatgc cttctcgatc ccgcatttgg ccgtaccatc
     1081 ggcctcgaag atcgtgtacg cgcattcctt accttttacc aatggcgtta cccactctcc
     1141 ggtgttgtaa cgcaggtaaa gcccttgttt ttcaatggct ttcaccccgg aggctggcag
     1201 atacggcttg actttcgggt acaccttctc caactccttc aattcctcct cttccagggg
     1261 agcgccataa tcgcctttaa cacaacaggc tcctttacag gcattcaggt cgcacacgaa
     1321 gtgcttttcg atgatctcgt cggagaccag ggtatcgccg atggctaaca tggggcaaag
     1381 ttagttgttt cgggttccga gttcataagt aggagcgagt tgcgagaccg tgaccgaggg
     1441 acgtgggacg agggacgtgg gacgagggac gagggacgtg ggacgaggga cgtggggcga
     1501 gggacgtggg acgtgggacg agggacgtga ccgaggtgcg aggaggatag ctttgcagca
     1561 accttcatcc ttcaactatg aaccgacccg aaagctgcct gtcttagctt aaacgcaggc
     1621 cgtgccacgg gcgaactgcc tactgcctac tgcctaccgc ctaccgccta ccgccatctg
     1681 cattccaccc aaccgctacc attcgatttg tcgtattttc gcgccatggc cagcacgaac
     1741 caggacgacc tctttaagaa catcatttcc cacgccaagg aatacggctt tattttccaa
     1801 tccagtgaga tctatgacgg actcagcgcc gtttacgatt acggccagaa cggcgccgaa
     1861 ctgaaaaaga acatccgcga atactggtgg aagtcgatgg tccagatgaa cgagaacatc
     1921 gtcgggatcg acgccgccat cttcatgcac cccaccacct ggaaggcttc cgggcacgtg
     1981 gacgcgttca acgacccgct ggtcgacaac aaagactcga aaaagcgcta ccgcgccgac
     2041 gtgctcatcg aagatcacgt ggccaagatc gaaggcaaga tccgcaagga agtcgaaaag
     2101 gcccggaccc gcttcggcga cagcttttcc gaacaccagt tccgcacgac caacgcgcgg
     2161 gtcctcgaat accagcaaca gatcgacacc atcaaccagc gcttcaaagc cgcgctggaa
     2221 gcgaacgacc tcaacgaagt ccgccagatc atcctcgacc tggagatcgt ctgcccgatc
     2281 tcgggcaccc gcaactggac cgaagtgcgt cagttcaacc tgatgttctc cacccagctc
     2341 ggttccgtca gcgaagaggc cagcaccatc tacctccgcc cggaaacggc ccagggtatc
     2401 ttcgtgaact tcctcaacgt ccagaagacc ggacggatga agatcccctt cggcatcgcg
     2461 cagaccggca aagcgttccg caacgagatc gtcgcccgac agttcatctt ccgcatgcgg
     2521 gaattcgagc agatggagat gcaatacttc gtcaagccgg gtacggagat ggagtggtac
     2581 aacttctgga aagaagcccg catgaagtgg caccgcgccc tcggcttccc cgacggcaag
     2641 caccgcttcc acgaccacct caagctggcc cactacgcca acgccgcctg cgatatcgag
     2701 ttcgagtttc cgttcggctt taaggagctg gaaggcatcc actcgcgcac cgacttcgac
     2761 ctgggcaatc acgagaaatt ctcgggtaag aaactacagt acttcgatcc cgaactgaac
     2821 cagaactacg tgccgtacgt cgtcgagacc tcgatcggtc ttgaccgcat gttcctggcc
     2881 gtgctctcca ccgcctatgc cgaagaacaa ctcgaagacg gcagcgaacg cgtcgtgctc
     2941 cgcatcccgc ctttcctcgc tccgatcaag tgcgcggtgt tgccgttggt gaagaaggac
     3001 ggtctgcccg aaaaggcgcg cgagatcatg gaccggctca agttcgacca cctctgcgtg
     3061 tacgaggaaa aagactccat cggcaaacgc taccgccgaa tggacgcgat cggaacgccc
     3121 ttctgcatca cggtcgacca cgacagcatc gaacaaaaca ccgtcaccct ccgcgaccgc
     3181 gacagcatgc aacaggaacg cgtttccatc gaccgcctgc cggagatcgt aggcgaaaag
     3241 gtcgatatgc ggaaggcgct gcggggactc tagggaggat cgagggacga gggacgaggg
     3301 acgagggacg aggggcgagg gcggttggcg gtgggcagta ggcggtgggc agtaggcggt
     3361 gggcggttgg cggtgggcgg ttggcggttg gcggtaggca gtaggcggtg ggcagtaggc
     3421 ggtgggcggt gattcaactt atatgtacta gcgcgaggta ctatatactg ttcgctattc
     3481 gctcctgcct acgcgaa
//
LOCUS       DAOTON010000216         3495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21244_length_3495_cov_2.695930, whole genome shotgun sequence.
ACCESSION   DAOTON010000216 DAOTON010000000
VERSION     DAOTON010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21244_length_3495_cov_2.695930"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="P5292_05410"
     CDS             complement(<1..751)
                     /locus_tag="P5292_05410"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRS38600.1"
                     /translation="MKRVLITGAAGFLGSHLCDRFLKEGYFVIGMDNLITGDLKNIEH
                     LFKEKNFEFYHHDVSKFVFVPGQLDYILHFASPASPIDYLKIPIQTLKVGSLGTHNLL
                     GLARVKNARILVASTSEVYGDPLVHPQHEDYWGHVNPIGPRGVYDEAKRFQEAITMAY
                     HTFHGVETRIVRIFNTYGPRMRLNDGRVLPAFIGQALRGEDLTIFGDGSQTRSFCYVD
                     DLVEGIYRLLLSDYHLPVNIGNPDEITIRDFA"
     gene            complement(762..2072)
                     /locus_tag="P5292_05415"
     CDS             complement(762..2072)
                     /locus_tag="P5292_05415"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRS38601.1"
                     /translation="MKIAVVGTGYVGLVTGTCFAETGNHVVCVDIDASKVARMQRGEI
                     PIYEPHLDVLFERNIKQGRLSFTTDLSEGIRDAQIIFLALPTPPGEDGSADLKYVLQV
                     AGQLGGLMRDYKIIVNKSTVPVGTAEKVRTAIAEKATTEFDVVSNPEFLREGFAVDDF
                     MKPDRVVIGTSSERARKLMAELYAPFVRQGNPIYFMDERSSELTKYAANAFLAMKITF
                     MNEIANLCEKVGANVDAVRIGIGADDRIGKRFLFAGIGYGGSCFPKDVQALEKIAEDA
                     RYDFRILDAVMAVNEDQKLSLIRKVKGLFPEGLRGKKVAMWGLAFKPDTDDIREAPSL
                     YMIEALLKEGASVSAFDPEAMENVRKQIGDKIQYGKDEYDVLDGADFLIIATEWAVFR
                     TPDFERMARSMKAKLIFDGRNLYDLNSIRERGFTYYSIGREPVL"
     gene            complement(2110..3243)
                     /locus_tag="P5292_05420"
     CDS             complement(2110..3243)
                     /locus_tag="P5292_05420"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRS38602.1"
                     /translation="MKKIQMVDLIGQYEKIQQEIDKAVLDVIRSSAYINGPEVKAFQA
                     ELEQYLGAKHVINCANGTDALQIAMMALDLQPGDEVITADFTYVATAEVIALLKLKPV
                     LVDVNPDTFTLDPAAVERAITPKTKAIVPVHLFGQCAPMEAIMDIARKYKLYFIEDTA
                     QAIGADYYFKDGSKKKAGTIGTIGCTSFFPSKNLGCYGDGGALFTNDDELAKKIRMIA
                     NHGQSVLYYHDVVGVNSRLDSIQAAILRIKLRHLDEYAAARRAVADRYDLAFANNPKV
                     KTPVRAQWSDHVFHQYTLVLNGVDRTKLREYLASKDIPAMIYYPVPLHLQKAYVDPRY
                     KAGDFPVTEHLCANVISLPIHTELSAETQDLIIQEVRSFLATS"
BASE COUNT          754 a          895 c          995 g          851 t
ORIGIN      
        1 cggcgaagtc gcggattgtg atctcgtcgg ggtttccgat gttgaccggg aggtgatagt
       61 cgctgagtag taaacgataa atgccttcga ccagatcgtc cacatagcag aacgaacggg
      121 tctggctgcc atcgccgaag atcgtcagat cttcaccccg taaggcttgt ccgatgaaag
      181 cggggagtac acgcccgtcg ttcaggcgca tgcgcgggcc ataggtgttg aagatgcgaa
      241 cgatgcgcgt ctcgactccg tgaaaggtat ggtacgccat cgtgatcgct tcctgaaagc
      301 gcttcgcttc gtcgtagacg cctcgcgggc cgatcgggtt gacgtgtccc cagtaatcct
      361 cgtgttgcgg atggacgagt ggatcgccgt acacttccga tgtggaggct accaggatac
      421 gggcattctt cacccgtgcg agtccgagga ggttgtgcgt acccagcgaa ccgactttca
      481 gcgtttggat cgggatcttg aggtagtcga tcggactggc tggtgaggcg aagtgcagga
      541 tgtaatcgag ctgaccgggg acgaagacga attttgaaac atcgtggtgg tagaactcga
      601 agttcttttc cttgaacaga tgctcgatgt tcttcaggtc gccggtgatc aggttgtcca
      661 tcccgatcac gaagtatcct tctttcaaaa aacggtcaca cagatgtgaa ccgagaaaac
      721 cggccgcgcc ggtgatgagt acgcgtttca tggtggatcg gtcacagcac cggttcccgg
      781 ccgatgctgt agtaagtgaa tccgcgttcg cggatgctgt tcaggtcgta gaggttgcgt
      841 ccgtcgaaga tgagtttggc tttcatcgac cgggccatgc gttcgaagtc gggtgtacgg
      901 aagaccgccc attccgtcgc gatgatgaga aaatccgcgc cgtcaagtac gtcgtattca
      961 tccttaccgt attgaatctt atctccgatc tgtttgcgca cattctccat cgcttccgga
     1021 tcgaacgccg atacgcttgc gccttccttg agcagcgctt cgatcatata aagagaagga
     1081 gcttcgcgta tatcgtcggt atccggtttg aaggcgagtc cccacatggc caccttcttt
     1141 ccccgcagac cttccggaaa gagtcctttc acctttcgga tgagggataa tttctggtct
     1201 tcgttcacgg ccatcaccgc atcgaggatg cggaagtcgt agcgcgcgtc ttcggcaatc
     1261 ttctcgaggg cctgcacgtc cttcggaaaa caactgccgc catagccgat gccggcaaag
     1321 aggaagcgtt tcccgatccg gtcgtcggcg ccgattccga tgcgaacagc gtccacgttg
     1381 gcgccgactt tttcgcaaag gttcgcgatc tcgttcatga aggtgatctt catggcgagg
     1441 aatgcattgg cagcgtattt cgtcagttcg gacgagcgtt cgtccatgaa gtagatcggg
     1501 ttaccctgtc gaacgaaagg ggcatacaat tccgccatca gcttgcgtgc gcgttccgag
     1561 ctggtcccga tcacgacacg atccggtttc atgaaatcat cgacggcgaa cccttcccgg
     1621 agaaattcag ggttggatac gacatcgaat tccgtagtag ctttttccgc gatagctgtt
     1681 cggacctttt cggcggtccc gaccggaacg gtgctcttgt ttacgatgat tttataatcg
     1741 cgcatgagtc cgccgagttg cccggcgacc tggagaacgt atttcaagtc ggccgagccg
     1801 tcctcgccgg gaggtgtagg aagagccagg aagatgatct gcgcatcgcg gataccttcc
     1861 gacaagtccg tggtgaagct gagtcggcct tgtttgatgt tacgttcgaa caagacatcc
     1921 aggtgcggtt cgtaaatcgg gatttcgcca cgctgcatac gcgcgacttt cgatgcgtcg
     1981 atatcgacgc ataccacgtg gttaccggtt tccgcgaagc acgtgccggt gactaagccg
     2041 acataaccgg taccaacgac ggcaattttc atgggtagct atttaaaagc cttttttact
     2101 agattccgat caggaagttg ctaaaaaaga gcggacttcc tgaatgataa ggtcctgtgt
     2161 ttcagcgctc agctcggtgt gtatcggcag cgagatcaca ttcgcgcaca aatgttcggt
     2221 aaccggaaaa tcgccggcct tgtaccgcgg gtcaacatac gccttctgca ggtgcagcgg
     2281 aaccgggtaa tagatcatgg cggggatatc cttcgaggcc aggtattcac gaagtttcgt
     2341 gcgatcgaca ccgttcagaa cgagtgtgta ctgatggaaa acgtggtcgg accattgagc
     2401 ccgtaccgga gttttaactt tcgggttgtt cgcaaaagcc agatcataac gatcggccac
     2461 cgcgcgacgt gcggcagcat attcgtcaag atgacgcaac ttaatgcgca ggatggctgc
     2521 ctggatggag tcgagtcgtg aattgacgcc gaccacatcg tggtaataga gtaccgactg
     2581 accgtggttg gcaatcatgc ggatcttctt cgcgagttcg tcatcgttgg tgaagagcgc
     2641 gccgccgtcg ccgtaacaac ccagattttt agacgggaag aatgaagtgc agccgatggt
     2701 tccgatggta cccgcttttt tctttgagcc atctttgaaa taataatctg cgccgatggc
     2761 ttgcgcggta tcttcaatga agtacagctt gtatttacgg gcgatatcca tgatcgcctc
     2821 catgggcgcg cactggccga acaggtgaac cggcacaatc gcctttgtct tcggcgtgat
     2881 cgcacgttcc accgccgccg gatcaagggt gaatgtatcg gggttcacat cgaccagtac
     2941 tggtttgagc ttgagcaacg cgatgacttc ggcggttgcc acataggtga aatcggcggt
     3001 gatgacttca tcgccgggct ggaggtccag cgccatcatg gcgatctgca gggcgtcggt
     3061 gccgttggcg caattgatca catgctttgc gccgaggtat tgttccagct cagcctggaa
     3121 cgcctttacc tccggcccgt tgatgtatgc cgaggaacgg atgacatcca gcaccgcttt
     3181 atcgatctcc tgttggatct tctcgtattg gccgatcagg tcgaccattt gaattttttt
     3241 cattcgccga tataaaagga ggccgaatat acggaatgtt cggccctccc tgtattgtgc
     3301 tgaatatcaa tagttaacat tcaagttgcc cgccttttcg aatggggaaa ggtcgctttt
     3361 tggtttgatt tccgattatc tttgccgact gtccagaccc gattatggcg aaaagcaaat
     3421 tcaccaaaga gcaatacctg aagtggtacg aagagttgtt gctcatgaga cgattcgagg
     3481 agcgtgcagg acaac
//
LOCUS       DAOTON010000217         3494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21248_length_3494_cov_3.193661, whole genome shotgun sequence.
ACCESSION   DAOTON010000217 DAOTON010000000
VERSION     DAOTON010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21248_length_3494_cov_3.193661"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..895
                     /locus_tag="P5292_05425"
     CDS             371..895
                     /locus_tag="P5292_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38603.1"
                     /translation="MMKRASMLTAFLLLVSLSCVLAQSLSPTVISPAGRFASAGGYTL
                     SETVGEMTMVETYSAGGSILTQGFQQPSDNNVRVPIVQQAGYDLTVSPNPGTGSFYFN
                     VAASAPVTIDIRVTDVIGQTVGIFQTSGIEGAQRLPVDLSGLRQGVYFAEVWMNYSTG
                     TTVYRMTHRLQVVR"
     gene            935..2677
                     /locus_tag="P5292_05430"
     CDS             935..2677
                     /locus_tag="P5292_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38604.1"
                     /translation="MNSRAFQFIATFLTCMLCLIGKQALAQSIAGVSAPPQMLNYQAV
                     ARDASGNALLSTAISLRFTIHSVSASGPIEYQETQSVTTNQFGLFSVQIGTGTVVSGI
                     FPLIDWVSADFFLQVEVDPTGGSSWIDMGAAQLISVPYSFVSKQSLISLDNHWVQNGP
                     NLYNEPLGKVGIGTSTPLYKLHVESDNSSSLTGVVHAQATHPTGSNLDVSAFYGISDV
                     DDYFGLGGTFYGGWKGVYGSVSPSGTGTYYGVNGSVYGGSGYNYGVYGESDQVGVYGD
                     ASGNGNGLTWIYGPSYDETAGLYGYASLNNNTNGISYGVVGESFGSSSNNNAGVFGYA
                     ANATGTSPRNWGVYGLSKDAVSAAGVLGVADGSGNSGHGVEGDALSGSNQIGVFGYAA
                     GTTGSDYAGYFLGQLYATTASSGIKAFKIDHPLDPENKYLFHSSVESDEMLNQYSGNV
                     TTDGQGLATVQLPDYFEALNKDIRYQLTVIGQFAQAIVQEEVSGNRFSIRTDKPNVRV
                     SWLVMGIRKDPLANQYRIQPVVDKPASEKGTYLVPQVYGKSIDRAPHQPTFLPYGNGN
                     RKVMKPMDDPKRKK"
     gene            2772..>3494
                     /locus_tag="P5292_05435"
     CDS             2772..>3494
                     /locus_tag="P5292_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS38605.1"
                     /translation="MNNHPRDLLRLASAFTPRRAFNAARVLANYQASRLTGKVVAPPF
                     PISLAIEPTTSCNLRCPECPSGLRSFTRPTGMLDPDFLRSVIDQVHRDVAYLVFYFQG
                     EPFLHPQFTDLVRYAKSKRIYTATSTNAHYLSPEKARETVASGLDRLIISIDGTTQDT
                     YSQYRVGGSLEKVLEGTRNIIEAKRKAGAATPYTVFQFLVVRPNEHQVQDVRNLAREL
                     GVDRVVLKTAQVYDFEHGNPLIP"
BASE COUNT          802 a          909 c          948 g          835 t
ORIGIN      
        1 ccggggtccg cagtcgtacc gatcgtggat ccggtcgaca ggaatccgtt gcacaagggg
       61 ttggcgttcg agcagtcatc ttgcgcggcg gcggtaaaat ggaggcagag caggagcagg
      121 gacgtgtaaa gtagacgcat tcggctttta gacggagtag tatttgggga atctccgaaa
      181 gtaccctgta acgggggagg gtaccctgta gttatatctc ttttattaac attgaaattg
      241 gatatttccc tttcccgagt gggcagcagt tgatgttcgt actggttttc cagagtaaaa
      301 aatgatgccg agaaaggcga gggtattcat acatgtttat ctttggggta cacaccttaa
      361 ccaccttacg atgatgaaac gggcttccat gctaaccgcc ttcttgctgc tggtttcttt
      421 gagctgcgta ctggctcaat ccctttcgcc aactgtcatt tccccggccg gcagattcgc
      481 ctcggccggt gggtatacgt tatcggaaac cgtcggagag atgacgatgg tcgaaacgta
      541 cagtgcaggc ggatccatcc tgacccaggg atttcagcag ccatccgata ataatgttcg
      601 tgtcccgatc gtacagcagg cgggttatga tttaacggtc tctccaaacc ccggcaccgg
      661 cagtttttac ttcaacgttg cagcctccgc gccggtaacg atcgatattc gggtgacgga
      721 tgtgatcggc cagacggttg gcatatttca gacttcgggc atagagggtg ctcaacgatt
      781 gcctgtggat ctttccggtc tccggcaagg tgtttatttt gctgaagtgt ggatgaatta
      841 ttccaccggc acaactgtgt atcggatgac gcaccgtttg caagtcgttc gataattttt
      901 tacccaaaac ctacttcacc taacccactt acccatgaac tcgcgcgcat ttcaattcat
      961 cgccaccttc ctgacgtgta tgctatgcct cattggcaaa caagcattag ctcaatccat
     1021 tgcgggtgtt tccgcaccac cgcaaatgtt gaattaccag gcagtcgccc gggatgcctc
     1081 cggcaacgca ctgctttcaa cggctatcag cttgcgcttt accattcaca gcgtttccgc
     1141 atcgggtccg attgaatacc aggagacaca atcggtcacg acaaatcagt tcggactctt
     1201 ttccgtccag atcggtactg gcaccgttgt cagcggtatt ttcccgctga tcgactgggt
     1261 ttctgcggat ttttttctgc aggttgaagt ggatcccaca ggcggttcat catggatcga
     1321 catgggtgcc gcacaattga tcagtgttcc atacagtttc gtttcgaaac agtcgcttat
     1381 ttccctggac aatcactggg tgcaaaacgg ccctaacctt tacaacgaac cgcttggaaa
     1441 agttggtatt ggaaccagta cgcccctcta taagttacat gtcgagagcg ataattcgtc
     1501 cagcttgacg ggtgttgttc atgcccaggc aacacatccg acgggttcaa atctcgatgt
     1561 ctccgctttc tacggcatca gtgatgtgga cgattatttc ggtttgggcg gtacttttta
     1621 tggaggatgg aagggggtat acggtagtgt atctccgagc ggaacaggta cttactacgg
     1681 ggtcaatggt tccgtttacg gtggctccgg ctacaactac ggtgtctatg gagaaagcga
     1741 ccaggtcggc gtctacggtg atgccagcgg aaatggtaat ggcctcacct ggatatacgg
     1801 accatcctac gacgaaacgg ccggtcttta cggatatgcg agcctgaata acaataccaa
     1861 tggaatcagt tatggggttg tcggagaatc gttcggtagt agcagcaata ataacgccgg
     1921 tgtattcggc tacgcagcca atgcgaccgg taccagtccg cgtaactggg gagtgtatgg
     1981 tttatcaaag gacgcggtat ccgctgcggg agtgctcggt gtggcggacg gatccggcaa
     2041 tagtggacac ggtgtcgaag gcgacgcgct cagcggcagt aaccagatcg gagtctttgg
     2101 ctacgcggcc ggaacgacag gttccgatta tgccggttat ttcctggggc aactgtatgc
     2161 tacaacggcc agctccggaa tcaaggcttt caagatcgat catccccttg acccggaaaa
     2221 caaatacttg ttccactcat ctgttgaaag cgatgagatg ttgaatcagt attcaggaaa
     2281 cgttacaacc gacggacagg gtttggcaac ggttcagtta cctgactatt tcgaagcgct
     2341 caacaaggat atccgttatc agctcacggt cattggacaa ttcgcacagg caatcgtgca
     2401 agaagaggtg agcgggaatc gattctcgat cagaaccgat aaaccgaatg tccgcgtgag
     2461 ttggttggtg atgggaatca gaaaagaccc gctggccaat caataccgta tccagccggt
     2521 ggtcgataag ccggctagtg aaaagggcac ctacctggtc ccgcaggtat atggcaagtc
     2581 gatcgatcgg gctcctcacc aacctacctt tctgccatac gggaatggaa accgaaaggt
     2641 gatgaagcca atggatgatc ccaagcggaa aaagtgagtt ggcagacctc ccgctcggtt
     2701 ggggttgaaa tagcaggatg tactgaacct atgtgttccg tccggaaacc cgcatctttg
     2761 caggtaccgc gatgaacaac catccccgtg acctgctgcg gcttgcctcc gcctttacgc
     2821 ctcgccgcgc cttcaacgcg gccagggtcc tggcgaacta tcaggcatcg cgtctgaccg
     2881 gtaaggttgt cgcaccgccc tttccgatca gcctggcgat cgaacccacg acatcctgca
     2941 acctgcgctg tccggaatgc ccgagcggtt tgcgctcgtt cacccgcccg accggtatgc
     3001 tggatccgga tttcttgcgt tcggtcattg atcaggtgca tcgggatgtc gcttacctcg
     3061 tattttattt ccagggcgaa cccttcctgc atccgcagtt caccgatttg gtccgctatg
     3121 cgaaaagtaa acggatctat acggcgacct ccaccaacgc gcattacctc tcaccggaaa
     3181 aggcccggga aaccgtcgcc tctggcctcg accgattgat catctcgatc gacggaacaa
     3241 cgcaagacac gtatagtcag taccgtgttg gcggatcgct ggagaaagtg ctggagggaa
     3301 cgcgcaacat catcgaggcg aaacggaaag caggagctgc aacaccctat acggtgttcc
     3361 agttcctggt cgttcgaccg aatgaacacc aggtacagga tgtccgaaat ctcgcgcgtg
     3421 aactgggcgt ggaccgggtc gtcctgaaga ccgcgcaggt gtatgatttc gagcatggca
     3481 acccgctcat accc
//
LOCUS       DAOTON010000218         3467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21448_length_3467_cov_2.706917, whole genome shotgun sequence.
ACCESSION   DAOTON010000218 DAOTON010000000
VERSION     DAOTON010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21448_length_3467_cov_2.706917"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="P5292_05440"
     CDS             complement(<1..525)
                     /locus_tag="P5292_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HRS38606.1"
                     /translation="MKSSIQRPEDSFKHQGMRRKMIADLQAKEIGNTRVWEAMLKVPR
                     HFFLDKAFLNQAYSDMAFRIGAGQTISQPSTVAYQSAILDVVKGEKVLEIGTGSGYQT
                     CVLLELGAKVFSIERQKELFDKTGPFLQSLGYSPKLFYGDGYKGLPAYGPFDKILVTC
                     GAPFVPQELVEQLKP"
     gene            655..1647
                     /locus_tag="P5292_05445"
     CDS             655..1647
                     /locus_tag="P5292_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRS38607.1"
                     /translation="MHKKLGIGLIGVGHLGKIHLRLILENPYLHLVGIYDHNDELARK
                     IEADFGVRRYENADQLIQDSEALDIVTPTLSHFDYASRAIRASRHIFIEKPLTHTVKE
                     GKKLVDLVMEAGVKAQVGHVERFNPAFLAAREYFGQPMFIETHRLAEFNPRGTDVSVV
                     HDLMIHDIDIVLSVVRSNVKKISASGVAVVSDTPDIANARIEFENGCVANLTSSRISL
                     KNMRKSRFFQRDAYISVDFYKKKTEVVRLKGLVGEPGPLDITIDLGEKGSKLIYFDQP
                     KVEEVNAIAMELDLFAKAIREDLPTPVPVEDGFAALSVAHQVNEKIQASLSVVA"
     gene            1644..2525
                     /locus_tag="P5292_05450"
     CDS             1644..2525
                     /locus_tag="P5292_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38608.1"
                     /translation="MKYPEQNNRSHLIGILLAAVAFSFWGCSKEDLESSVPGYLYIDT
                     IHLTTDEISQGNNRAAFTDAWVFVDNEPLGVFPLPATVPVLDAATHKISIRAGIVENG
                     IDALRSAYPKLVPFDTTISLLPNRTSAFQPRVRYLNSAQFVQLENFDGGSVSLESIQT
                     NGAPIRISIAGDPNVLDGAAGVVDLDATQPSFQLATSDTFLLPTSVSSYIEMNYRCDV
                     EFTVGVIVSSTGGTVSFNSLVTLRPTTTWKKVYVNVADLGGVQTDATGYKLWLSGTKP
                     NSVSQASLYFDNIKVLY"
     gene            2525..>3467
                     /locus_tag="P5292_05455"
     CDS             2525..>3467
                     /locus_tag="P5292_05455"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRS38609.1"
                     /translation="MSKRGYTILSVCFDAMAAAAAWALFFVYRKSVIETEKFGYDVFM
                     LDQKFYLGILVIPSFWLLLYWVTGTYRNVLRKSRLKEFGQTLGISLIGVLVIFFSLLL
                     DDTVISYKTYYLTFLTLFLLHFVITAALRLFISTRIIRDIYNKRIGFNTIMIGSNQKA
                     LELLRQMETEFRSEGHTFVGFVHVDGNNDHLLGRHLQNLGGILDLREIITRHQVEEAI
                     IALESSEHENIGKVINELEDTPVIVRIIPDMYDILSGSVKMNAIFGAPLIEISPDLMP
                     TWQLAVKRIFDIIISVFVLTFFFPLYLFTAIGVLLSSR"
BASE COUNT          816 a          974 c          849 g          828 t
ORIGIN      
        1 gggcttcagt tgttctacca actcctgggg aacgaacggg gctccacacg taacgaggat
       61 cttgtcaaat ggtccgtaag caggtaagcc tttgtagcca tcgccgtaga acaatttcgg
      121 actataaccg agcgactgta ggaatggccc tgttttatcg aacaactctt tctgtcgctc
      181 gatagaaaat accttggcac ccaactccag caaaacacag gtctgatagc ccgaaccggt
      241 tccgatctcc agcactttct cccctttcac tacgtccaga atggctgatt gataggcaac
      301 cgtggaaggc tgagagatgg tttggcctgc tccgatcctg aaagccatat cagagtatgc
      361 ctgattcaaa aaggctttat cgaggaagaa atgccgggga actttcaaca tggcttccca
      421 gacacgggta ttgccgatct ctttggcctg caggtcggca atcatcttac gacgcatgcc
      481 ctggtgctta aaactgtctt ccgggcgctg aatggaggat ttcataaagt ctgacaaaga
      541 aacgatgaat gaacctccca ttttgcccac atttgaacga atttcaacac tctggcgttg
      601 atcctccctt tccccacccc tctgaaattc ctaccttcgc gcctctcctg aattatgcat
      661 aaaaagctgg gcatcgggct aattggcgta ggtcacctgg gtaagatcca cctccggctg
      721 atcctggaaa atccctacct ccacctggtt gggatctacg accacaacga cgagctcgcg
      781 cgcaagatcg aagctgattt cggcgtccgg cgttatgaaa atgccgatca actcattcag
      841 gattccgaag cgctggacat tgtaacgcct accctgtccc acttcgacta tgcatcaaga
      901 gccatacggg catcccgtca catcttcatc gagaaaccgc tgacccatac cgtcaaggaa
      961 ggtaaaaaac tcgttgacct ggtgatggaa gccggcgtca aggcccaggt tggacatgtg
     1021 gaacgattca acccggcttt tctggcggcc agggagtact tcgggcaacc gatgttcatc
     1081 gaaacgcacc gccttgccga attcaatccg cggggtacgg acgtgtcggt cgttcacgat
     1141 ctgatgatcc acgacatcga cattgtcctg agtgtcgtgc gttccaacgt gaagaagatc
     1201 agcgccagcg gtgtggccgt ggtcagtgat accccggata tcgccaatgc gcgcatcgag
     1261 tttgaaaacg gttgcgtcgc caaccttacc tccagcagga tatccctgaa gaacatgcgg
     1321 aagagtcgct tctttcagcg cgatgcctac atctccgtcg acttctacaa gaagaaaacc
     1381 gaagttgttc gcttgaaggg acttgttggc gaacccggac cgctcgacat cacgatcgat
     1441 ctgggtgaaa aaggatccaa gctgatttac ttcgaccaac cgaaagtgga agaggtgaat
     1501 gccattgcca tggaactcga cctgttcgcg aaagcgatcc gcgaagacct gcccactccg
     1561 gttccggttg aagacggctt tgccgcgctc tccgtcgctc atcaggtgaa cgaaaagata
     1621 caggcatccc tgtctgtcgt agcatgaagt acccggagca aaacaaccga agccatctga
     1681 tcggcatcct gcttgcagcc gttgcatttt cattctgggg gtgttcgaag gaggaccttg
     1741 agtcttcggt tcccggctat ctctacatcg acacaatcca tctgacaacc gacgagatca
     1801 gccagggcaa caaccgcgct gcttttaccg atgcctgggt attcgtggac aatgaacccc
     1861 tgggagtttt cccgctaccg gccaccgtgc cggtgctgga tgccgcgacg cacaagatca
     1921 gcatccgggc aggcatcgtc gagaacggta ttgacgccct tcgctccgct taccccaaac
     1981 ttgtcccgtt cgacactact atttcacttt taccaaatcg cacctcggcc ttccaaccca
     2041 gggtccgcta tctgaacagt gcgcaattcg tacaattgga aaatttcgac ggaggatccg
     2101 taagtctgga aagcatccag acgaatggag cgccgatccg gatcagcata gccggcgatc
     2161 ccaatgtcct cgatggtgcc gccggcgtgg tggaccttga cgctacgcaa cccagtttcc
     2221 aacttgccac cagcgataca ttcctgttac cgacatcggt atcctcgtac atcgaaatga
     2281 attaccgttg tgatgtggaa ttcaccgtcg gagtcattgt cagttcaacc ggcggaaccg
     2341 tttcgttcaa ttcccttgta accttacgac cgacgaccac ctggaaaaaa gtttacgtga
     2401 acgtcgccga cttgggcggc gtccaaactg atgccaccgg ttacaaattg tggctgagcg
     2461 gaacaaaacc aaattcggtg tcacaggcat ccttgtactt cgacaacatc aaggttcttt
     2521 actgatgagt aaacgcgggt acacgatcct ctctgtctgc ttcgacgcca tggccgccgc
     2581 tgcagcctgg gcgctatttt ttgtttaccg aaagagtgtg atcgaaaccg agaagttcgg
     2641 atatgacgtc ttcatgctcg atcagaaatt ctacctcggg atactggtca tcccttcctt
     2701 ctggctgcta ttatattggg tgaccggaac ctaccgaaat gtcctgcgca aaagccgcct
     2761 gaaagagttc ggtcagacgc tgggcatctc cctgatcggc gtactggtca tctttttttc
     2821 cctgctgctc gacgataccg tcatttcata caaaacctat tacctgacct ttctgacgct
     2881 tttcctcttg cactttgtta tcacggcggc gcttcgtctt ttcatttcaa cccgcattat
     2941 ccgggatata tacaataagc ggatcggttt caatacgatc atgatcgggt cgaaccagaa
     3001 ggccctggaa ctcctccggc agatggaaac ggaattccgg agcgaaggcc atacgtttgt
     3061 cgggttcgtt catgtcgatg gaaacaacga ccacttattg ggaagacacc tgcagaacct
     3121 ggggggtatc ctcgatttgc gggagatcat tacccgtcac caggtggaag aagcgatcat
     3181 tgcactggaa tcttcagaac acgagaatat cggcaaggtc atcaatgaac tggaagacac
     3241 gccggtcatc gtccggatca ttccggacat gtacgatatt ctttcgggct ccgtaaagat
     3301 gaacgcgatc ttcggggcgc cgctgatcga gatctcgccg gacctgatgc ccacctggca
     3361 actggcggtc aagcggatct ttgatatcat catttccgtt tttgtcctga ccttcttctt
     3421 ccccctctac ctgttcacgg ccatcggcgt gttgctttca tcccgtg
//
LOCUS       DAOTON010000219         3457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21516_length_3457_cov_3.444444, whole genome shotgun sequence.
ACCESSION   DAOTON010000219 DAOTON010000000
VERSION     DAOTON010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21516_length_3457_cov_3.444444"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="P5292_05460"
     CDS             <1..484
                     /locus_tag="P5292_05460"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRS38610.1"
                     /translation="VPIHLPGKTEIDIPGLGLITIFLFIAAVGWLSARYIRNPLFSYL
                     EQLIDKAPLVKLIYSSVKDLVQAFVGEKRRFNQPVLVRLEKESEINRIGFITQEDLSD
                     LGLEKEKVAVYLPFSYSFAGELIILPRENIRPLNASGTDMMKFIISGGVTEIKEDGKK
                     "
     gene            543..1526
                     /locus_tag="P5292_05465"
     CDS             543..1526
                     /locus_tag="P5292_05465"
                     /inference="COORDINATES: protein motif:HMM:NF036967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HRS38611.1"
                     /translation="MSFRNVFKQDETREFGFGSRVEGRDQRLMNRDGSSNVRRKGLPY
                     FQRFNVFHELIRMAWWKFFTILFSTYLVINLIFASLYCFTWPDSIGGMIYQSEWEKFL
                     EIFFFSAQSLTTVGYGRLNPTGVFDSALASIEAMVGLLGFAVATGLLYGRFSRPVVRL
                     LYSEQAVVAPYRDRSALMLRVAKKHRSELIDIQASIVMSYVLQEAGKPVRKFANLKLE
                     FTRVNLLTTSWTIVHPIDDESPMLGWSADDFVNNRTELLVLLQAYEETFANNVHSRTS
                     YKANEIVVGARFISMLDAGEEGSVAVHLDRISAYEPAALPVESGSGRSSLS"
     gene            1665..2018
                     /locus_tag="P5292_05470"
     CDS             1665..2018
                     /locus_tag="P5292_05470"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRS38612.1"
                     /translation="MMIRVLKSKIHRVKVTEADLNYVGSITIDEDLMDAAGLIENENV
                     DVLNVNNGERFDTYVIKGKRGTGQICLNGPAARKAMVGDVVIVVSYALMDFEEAKTFK
                     PTLIFPDAHNRVHPS"
     gene            2021..3037
                     /locus_tag="P5292_05475"
     CDS             2021..3037
                     /locus_tag="P5292_05475"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRS38613.1"
                     /translation="MQKKALSIFRNLLLLAIAGLLLWWSFRGVDLRATLSEFSEIRWG
                     WLLLSVAASLIAFLSRAERWRLLMRPLGYHPGLGVTSLSLMTGYLANLALPRLGEVTR
                     CGSLSRATGVPFDRLLGTVIVERVIDVLSLAACMLGTALLERERLGGFLHDNLFGPLG
                     EKLSGLFGNPFVWATLVAVTLFIIWWKRRSRAAGTPKAGFLQRLTGMLQGILEGFRSV
                     RKLDRPWAFLFHTVLIWVMYFLMSYTCFFALPATDQLSPAAGLFVLVVGGMGMSAPVQ
                     GGIGAYHLLVSRGLMLYGLTQTHGLAFATLMHTSQTLVVLVLGSICFGILVFMQRKQV
                     DGNA"
     gene            3024..>3457
                     /locus_tag="P5292_05480"
     CDS             3024..>3457
                     /locus_tag="P5292_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRS38614.1"
                     /translation="MATLDIIRRKILAPEQVANWVSLQRFLSKRIVFTNGCFDILHLG
                     HVDYLARAADEGDVLVIGLNTDESTRRLKGPHRPINDEVQRAMLLASMHFVDAVIFFG
                     EDTPLELIRKVMPDVLVKGADYSLENIVGADLVLASGGQVKT"
BASE COUNT          681 a          995 c          979 g          802 t
ORIGIN      
        1 ggttcccatc cacctgcccg ggaaaaccga gatcgacatt cccggcctgg gcctcatcac
       61 gatcttcctc ttcatcgcgg cggtgggctg gctgtcagca cgctacatcc gcaatccgct
      121 cttcagctac ctcgagcaac tgatcgataa ggcgcccctg gtcaaactca tttactcctc
      181 cgtgaaagac ctggtgcagg cattcgtcgg tgagaaacgt cgtttcaatc aacctgtcct
      241 cgtccgactg gagaaggaat ccgagatcaa ccgcatcggt ttcattacgc aggaagacct
      301 cagcgatctc gggctggaaa aggagaaggt cgccgtctat ctgcccttct cctactcgtt
      361 cgccggggaa ctgatcattc tgccgcgaga gaacatccgg ccgttgaatg cttccggtac
      421 cgatatgatg aagttcatca tcagtggcgg cgttaccgaa atcaaggaag acggaaagaa
      481 gtaacggact cccgggctca gtccggttat cgcatgcagc atcagtagtc gattcgatca
      541 tcatgtcctt tcgtaacgtt ttcaagcagg acgaaacacg cgagttcggt ttcggtagcc
      601 gtgtggaagg ccgcgaccag cgtctgatga accgcgacgg ttccagcaat gtccgccgga
      661 aaggcctccc ctattttcaa cggttcaacg tctttcatga attgatccgg atggcgtggt
      721 ggaaattctt cactattctg ttctcgacct atctggtcat caacctgata ttcgcatcgc
      781 tctactgctt cacctggccg gacagtatcg gcggtatgat ctaccagagt gaatgggaga
      841 aattcctcga gatctttttc ttcagcgcgc agtcgcttac gacggtcggt tacggccggc
      901 tcaacccgac gggcgtgttc gacagcgcgc tggcttcgat cgaagccatg gtgggcctgc
      961 ttgggttcgc cgtcgcgacc ggtttgttgt acggacggtt cagccgaccg gtcgtccgtt
     1021 tgttgtacag tgaacaggcg gtggtggcgc cctatcgcga ccgcagcgcg ctgatgctgc
     1081 gcgtcgcgaa aaagcatcgg agcgaactca tcgacatcca ggcaagcatc gtgatgtcgt
     1141 atgtgttgca ggaagccggg aagccggtgc gaaaattcgc caacctgaag ctcgagttca
     1201 cgcgcgtcaa cctgctcacc acatcctgga ccatcgtaca cccgatcgac gacgaaagtc
     1261 cgatgctcgg ctggagtgcc gacgacttcg tgaacaaccg tacggagctg ttggtgctgt
     1321 tgcaggcgta cgaagagacc tttgccaaca acgtgcattc ccgcaccagc tacaaggcca
     1381 atgaaatcgt ggtgggagcc cgattcatct ccatgctgga cgccggtgaa gaaggatccg
     1441 ttgccgttca cctggaccgg atctccgcct acgagccggc ggcacttccg gtcgaatccg
     1501 gttccgggcg ttcttctctt tcgtgaaacg cctttcgtcc gcctgcgtat gagcagcagg
     1561 gttcgcaaca atgccggaac ctggtcaata ttctgaaatt caattaatta cctgttcaca
     1621 aggtttggcc gaatgcgctg aagtccgtag gtttgcagcc cgctatgatg atccgagtac
     1681 tgaaatccaa aatacaccgc gtcaaggtaa ccgaagccga tctcaactat gttggcagca
     1741 tcaccatcga tgaagacctg atggatgccg ccggactgat cgagaacgag aatgttgacg
     1801 ttctgaacgt caacaacggc gagcgattcg atacgtacgt gatcaagggc aagcgcggca
     1861 ccggacagat ctgtctgaac ggtcctgccg cgcgtaaagc aatggtcggc gacgttgtca
     1921 tcgtcgtctc gtatgcgctg atggattttg aagaagcgaa gacgttcaag ccgacgctga
     1981 tcttcccgga tgcacacaac cgcgtgcatc cttcctgatc gtgcaaaaaa aagcgctgtc
     2041 cattttccgc aacctgctgc tcctggcgat cgccggattg ctgctgtggt ggtcgttccg
     2101 gggtgtcgac ctacgcgcta ccctttccga attttccgaa atccgttggg gctggttgtt
     2161 gctctccgta gccgcgtcgc tcattgcctt tctcagtcgt gctgaacgct ggcggttgct
     2221 gatgcgcccg ctggggtatc atcccggact gggcgtcacc tcgctcagcc tgatgaccgg
     2281 ctatctcgcc aacctggcct taccccgctt aggtgaggtg acccgctgcg gttcgctcag
     2341 tcgcgccacc ggcgtgccct tcgaccgact gcttggtacc gtgatcgtag agcgagtcat
     2401 tgatgtgttg tccctcgccg cgtgcatgct gggtaccgcc ttgctggaac gcgaacggct
     2461 cggtggattt ctccacgaca accttttcgg accgctcggc gagaagctgt cggggctttt
     2521 cggtaatccg ttcgtgtggg cgacgcttgt cgccgtcacg ctcttcatca tctggtggaa
     2581 gcgacggtcc cgtgccgccg gcaccccgaa ggcaggattc ctgcaacgtc tcaccggaat
     2641 gttacagggc attctcgaag gattccgttc cgtacgcaag ctggatcgtc cgtgggcatt
     2701 tctctttcat acggtgctga tctgggtgat gtacttcctg atgtcgtaca cctgtttctt
     2761 cgcgctaccc gcaaccgatc agctcagtcc ggcagccggc ttgtttgtcc tggtcgtcgg
     2821 cgggatgggc atgtcggcgc ctgtacaggg aggcattggc gcctatcacc tgctggtttc
     2881 ccgcggactg atgctatacg gattgacgca gacacacggg ctggccttcg ccaccctgat
     2941 gcatacctcc cagacactgg tggtcctcgt cttgggtagc atctgtttcg gtatcttagt
     3001 gttcatgcaa cgcaaacagg tcgatggcaa cgcttgatat catccgccgg aaaattctcg
     3061 caccggagca ggtcgccaat tgggtaagcc tgcagcgctt cctgagtaag cgcatcgtct
     3121 tcaccaacgg atgcttcgat atcctgcacc ttgggcacgt cgattacctg gcgcgtgcgg
     3181 cggatgaggg cgacgtcctg gtcatcggtt tgaatacgga tgaaagcacc cgccggctga
     3241 aaggtccgca ccgtccgatc aacgatgaag tgcaacgcgc gatgttgctg gcgagtatgc
     3301 acttcgtgga cgcggtgatc ttctttggtg aagacacccc gcttgagctg atccgcaagg
     3361 tcatgccgga tgtgttggta aaaggtgccg actattcgct ggaaaacatc gtgggagccg
     3421 atcttgtatt ggcctccggc gggcaggtca agaccat
//
LOCUS       DAOTON010000220         3453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21553_length_3453_cov_3.101236, whole genome shotgun sequence.
ACCESSION   DAOTON010000220 DAOTON010000000
VERSION     DAOTON010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21553_length_3453_cov_3.101236"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="P5292_05485"
     CDS             <1..954
                     /locus_tag="P5292_05485"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRS38615.1"
                     /translation="PAFNLKDDEIKAMLAYIKTEAEKPDAVAGPKGPAGGAAQEAPKP
                     IGLYLIIAIVIIGAIAMVLRRVQDTLARTVRQKQGLPEPAPMTRSQAFVHWSRNNKKL
                     VAVILIFLALYGSVKGWYALQSVGIHQGYLPEQPIKYSHQLHAGKLQIQCIYCHSGAE
                     KGKVAGVPSANVCMNCHKYVRKGPETGTEEIAKIYKALDYDPDKGTYGPNPKPLQWVR
                     VHNLPDLAYFNHAQHVKVGQVACQTCHGPVQDSMTVASQYSPLTMGWCIDCHRKTPVK
                     MAGNGYYAGYDHSKLIHDRNTPDSVITVAKIGGLECSRCHY"
     gene            1019..>3453
                     /locus_tag="P5292_05490"
     CDS             1019..>3453
                     /locus_tag="P5292_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HRS38616.1"
                     /translation="MSQRYWKGVEELQNDPEFARLKNNEFYEHLPLDSMISKKAEDVS
                     TTPRRDFLKFLGFSVAAASLAACETPVQKTIPYLIRPDQITPGIPNWYASTYFDGYDY
                     CSVLVKTRDGRPIKIEGNTLSSVTRGGVSARVQASVLSLYDSGRLKSALASGKEAAWA
                     DIDKAITSKLAEIAAAGGKIRILSSTVISPTVSKAIADFAAKYPTTQHVQYDAFSASA
                     LRQAHNEGFGVNAVPSYDFSKADVIVGIDCDFLAHWVSPIEHARQYAETRKLDEGKKT
                     MSRHIQYEAALSLTGSNADQRHGMKPSMSGAVAANLYNAVAAKAGATAVSAPSAGELS
                     KAIEKTAEELWAAKGKSLVVCGTNDLATQHLVAGINSLLENYGTTVDLDNTNRMRQGN
                     DTALATLLDELGKGEVAALFVYNANPVYTLPNGTAVGEMIRKAGLTVSFADRNDETAA
                     CCQYVCPDHHALESWSDAEPRSGAYSLGQPTISPLFQTRPVVESLLAWAGSTMAAHDY
                     MKQVWASAILPKAGVSWEKALQEGVVELAKATPRSAKTSANLSAAASALPKAGSGIEL
                     TVYEKVGLGNGQQANNPWLQELPDPISKVTWDNYLAVSPKDAREKGWKQGNVVQVKAG
                     NVTIKAPVVIQPGQTPGTVSIAAGYGRTAAGKTANGVGVNAYPLTSLSNGQISYAVAG
                     VDVQKTADDDHALAATQTHHTMMGRAIVKETNLAEWTKDPKAGNEEELFPVFVGKEKT
                     EQPASQVSLWNEFQDGHHHWGLAIDLNSCIGCGACVVACTSENNVSVVGKDEVMRSRE
                     MHWMRIDRYYSSDA"
BASE COUNT          775 a         1029 c          979 g          670 t
ORIGIN      
        1 ccggccttca atctgaagga cgacgagatc aaagccatgt tggcttatat caagacggag
       61 gctgagaagc cggatgccgt cgccggtcct aaaggccccg ccggaggcgc tgctcaggaa
      121 gcaccgaagc cgatcggttt gtacctgatc atcgcgatcg tgatcatcgg cgccatcgcc
      181 atggtgttgc gtcgcgtaca ggacacgctt gcccgtacgg tccgtcaaaa gcagggcttg
      241 ccggaaccgg cgcccatgac ccgttctcag gcattcgtcc actggtcacg caacaacaag
      301 aaactcgtcg cggtcattct cattttcctg gcgctttacg gaagtgtcaa aggatggtac
      361 gctttgcaat cggtcggtat ccaccagggc tatctgccgg aacagccgat caagtattcg
      421 caccaactgc atgccggcaa gctgcagatc cagtgtatct actgccacag cggcgctgaa
      481 aaaggaaaag tagccggtgt tccttcggcc aatgtgtgta tgaactgtca caagtacgta
      541 cgcaaaggtc cggaaacggg taccgaggag atcgccaaga tctacaaggc gctcgactac
      601 gatcccgaca agggcaccta cggtccgaat ccgaagccgc tgcagtgggt tcgtgtgcac
      661 aacctgccgg atctcgccta tttcaaccac gcgcaacacg tgaaagtcgg acaggttgcc
      721 tgtcagacct gtcacggtcc ggtacaggac agcatgaccg tcgcttcgca gtactcaccg
      781 ctcaccatgg gctggtgtat cgactgtcac cgcaagacgc cggtgaaaat ggccggtaac
      841 ggttactatg ccggttacga tcattccaag cttatccacg atcgcaatac acccgactcg
      901 gtcatcacgg tagccaagat cggtggtctg gaatgttccc gttgtcacta ctaatagaac
      961 agttaccgtc gcgggccttc cggttcgcga cgtttgcgca taatctgccg tacacgcaat
     1021 gagccaacgc tactggaaag gtgtagagga actccagaac gatccggagt tcgcccgcct
     1081 aaagaacaat gagttctacg agcatctgcc gctggattcg atgatcagca agaaggcgga
     1141 agatgtcagc acgacgcccc gtcgcgactt cttaaagttc ctgggcttca gtgtcgcggc
     1201 cgcctcgctg gcagcctgtg agacgccggt gcagaagacg attccgtacc tgatccgtcc
     1261 cgaccagatc actccgggta tcccgaactg gtacgcctcg acctatttcg acggctatga
     1321 ctactgcagc gtgctcgtca agacgcgcga cggtcgtccg atcaagatcg aaggcaatac
     1381 gctgagctcc gtcacccgtg gcggtgtcag cgcccgcgta caggccagtg tgctttcgct
     1441 gtacgacagc ggtcgcctga agtctgctct tgcttcgggt aaagaagccg cctgggccga
     1501 cattgacaag gccatcacgt ccaaactcgc tgagatcgcc gctgctggcg gtaagatccg
     1561 catcctgtct tcgactgtta tctcgccgac cgtctccaag gcgatcgccg atttcgcagc
     1621 gaagtacccg accacccagc acgtacaata cgacgctttc tctgcctccg ctttgcgcca
     1681 ggcgcacaac gaaggattcg gtgtcaatgc tgttccttcc tatgatttct ccaaagccga
     1741 tgtgatcgtc ggtatcgatt gcgacttcct cgcgcactgg gtttctccga tcgagcatgc
     1801 ccgtcagtac gccgagaccc ggaaactgga cgaaggcaag aagacgatgt cgcgtcatat
     1861 ccagtacgaa gccgcgctga gcctcaccgg ctcgaacgcc gaccagcgtc acggtatgaa
     1921 gccgtcgatg tcaggagccg ttgccgccaa cctgtacaat gccgtcgccg cgaaagccgg
     1981 cgctacggcg gtaagcgctc cctcggctgg tgaactttcc aaggcgattg aaaagaccgc
     2041 ggaagaactc tgggctgcca agggcaaatc actggttgtc tgcggcacca acgatcttgc
     2101 aacgcagcac ctcgtcgctg gtatcaacag cctgctcgag aactacggta ccacagtaga
     2161 tcttgacaat acgaaccgca tgcgtcaggg taacgacact gcgttggcca ccctgctcga
     2221 tgaattgggc aaaggtgaag ttgccgcgct gttcgtttac aatgcgaatc cggtatacac
     2281 cctgccgaac ggcactgccg tcggcgagat gatccgcaag gcaggtctca ccgtatcgtt
     2341 tgccgaccgc aacgacgaga ccgcagcctg ctgccagtat gtttgcccgg atcaccacgc
     2401 actggaatcc tggtccgacg cggaaccccg cagcggagcg tattcactcg gtcagccgac
     2461 catcagtccg cttttccaga cccgtccggt tgtggaatcg ctgctggcct gggccggtag
     2521 caccatggct gctcatgact acatgaagca ggtatgggct tctgccattc tcccgaaggc
     2581 aggcgtatcc tgggaaaaag cactgcagga aggtgtcgtg gaattggcga aagccactcc
     2641 gcgcagcgcg aagacctcgg ccaacctcag cgccgccgct tccgcgcttc ccaaagcagg
     2701 ttcgggcatt gaattgacgg tttatgaaaa agtcggattg ggcaacggtc agcaggcgaa
     2761 caacccctgg ctgcaggaac tgccggatcc gatctccaaa gtcacctggg acaattacct
     2821 cgccgtatcg ccgaaagacg cccgtgaaaa agggtggaag cagggtaatg tggtgcaggt
     2881 aaaagcgggt aacgttacga tcaaggcccc ggtggtgatc cagccgggtc agacgcctgg
     2941 aaccgtttcg atcgccgccg gttacggccg taccgctgcc ggtaagacgg ccaacggggt
     3001 gggggttaat gcatatccgc taacttcctt gtccaacgga cagatcagct acgctgtcgc
     3061 cggtgtcgac gtgcaaaaga ccgccgacga tgaccacgcg ctcgcggcta cccagaccca
     3121 ccacacgatg atgggtcgcg cgatcgtgaa agagaccaac ctggctgagt ggaccaaaga
     3181 tccgaaggcg ggtaacgaag aagagctctt cccggtattc gttggaaaag aaaagaccga
     3241 acaacctgcc agccaggtat ccctgtggaa tgaattccag gatggtcatc accactgggg
     3301 actggccatc gacctgaact cgtgtatcgg ctgcggcgcc tgtgtggttg cctgtacgtc
     3361 cgagaacaac gtttcggtgg tcggtaagga cgaagtgatg cgcagccgcg aaatgcactg
     3421 gatgcgcatc gaccgttact actcgagcga tgc
//
LOCUS       DAOTON010000221         3450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21577_length_3450_cov_2.661856, whole genome shotgun sequence.
ACCESSION   DAOTON010000221 DAOTON010000000
VERSION     DAOTON010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21577_length_3450_cov_2.661856"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..>3450
                     /locus_tag="P5292_05495"
     CDS             352..>3450
                     /locus_tag="P5292_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRS38617.1"
                     /translation="MYVDRFWKQKWNSNTEVDPTFSILTVDEDHDGIFEKEDALLRYA
                     AENHITYLALYDIYRILGRSYTAWNGSTRRMENLEDHLCRFIKKAKEQYGITQIGLIG
                     GSDVFFDSLDTYMDRFPPTAPLRLPGSLKNRPGLPAILKKLEEPLVEGDPTTPLAEHL
                     KLMLRVQQFNESNPCGVDLDVVNMEYEFWGNCATELPIFTEYIQAMDAFRDQYDSLHT
                     EHPLITEAYVAFLTYCNFPNVVAIMDGCSNCSPCPTCNNPHEPLIDRVLYSQQIMQPN
                     YFLLADQSHFEAPTSQDSTDFHPLLYAESIQTGGGADYLGAWFPQSPSFTIFTAESRY
                     YSAWRNYTTTPFGQPRQNDVQRGGAMWFASSHMMDWLDHPVILQNLGPFCTQSDTQQV
                     EMKYFGPTEPGIGYRFHIFRDSDSTIVYPTNGNPLAGITQPYEPSVGGSREIMAVDFS
                     DTLLFPRIRLAHGTYTSVLELDYDSASNAGYTARYPVVVSDQPQIVLMNEPEFCAGGY
                     CYLVTNSGGSQYTWTRDNIVVKSGTEWWVKATEDGYYKCVISGAPGSCSGTTDSVRIQ
                     VMPMPVVVVNALCNGDGTVTLKTDMRPANPNSTTTSGPGGVTYKWNTGATTDQITITP
                     TSSNTYRVLVTDPYSGCSTYRDCYVAAPPSNSFTPNIVVNSVPTSACSSNGSLTATLS
                     PGSYLGQTAYLWNTGQTTQTITNLPPGTYIVSMSYWKSACTYYDTVQLGTPTTGGPSI
                     SEVIIPSGCSNTASGSVQLMLTGGQPPFSFFWFGIPADGIHHPANQDQSNLFPGIYTI
                     RVRDVNGCEFWHEVTVPSGNSIIRVQPVSILPSSNCFNSPNGSATVSASGGTGPYQFD
                     WNDPLGQQGATADSLVAGSYWVTVTDANGCTSSTFIDIPATSLPINLTELASESLPAT
                     CQPGNDGVAALVVQGGVVPYTVNSPWTLDTLGTARLTGTPGNYPILVTDANGCQFTDT
                     ITIHTTEPPSFSLSALGTSCSGCTDGAVAINNIVTNSNWNVQWTPQLGTLSGDSLIQM
                     PGG"
BASE COUNT          862 a          983 c          791 g          814 t
ORIGIN      
        1 tcctctccac ccgattgcac tgatgcctgg caggatcgaa tgtatctggg agcacccgat
       61 cgagaccagt gggatgactg gacaagacgt cccccggttg cgggaaactg ttaaaaaact
      121 tatgctaaaa caactggaag aagttgctcg cctacggcag aaatgaattt gcccatcaag
      181 tcggcacatt gaccacattt ctgaatatga cacgaaccgc taaggtcaat tcgctacatt
      241 tcattttctt ttgacgttgc aatgcggaca ctaactacca gcttccttct tttttttctt
      301 tgcacatcgg cacttgctca attcagtccg gaacacgacc cacatcgagg catgtatgtg
      361 gatcgctttt ggaaacagaa gtggaacagc aatactgaag ttgatccaac attttccatc
      421 ctcaccgttg acgaggacca tgacggtatt tttgagaaag aagacgcatt actccggtat
      481 gccgctgaga atcatatcac ctacctggcg ctgtatgaca tttaccgaat tctggggcgt
      541 tcctataccg cgtggaacgg ctccacccgt cggatggaga atttagaaga ccacttatgc
      601 cggttcatta aaaaagcgaa ggagcaatac ggaattactc agattggcct gatcggcgga
      661 tcggatgttt tcttcgacag cctcgacacc tatatggatc gattcccgcc gactgctcca
      721 ctgcgactac cggggtcatt gaaaaaccgc cccggacttc cggccattct aaaaaaattg
      781 gaagagcctt tggttgaagg agatcccaca acacccttgg cggagcatct taaactgatg
      841 ctgcgggttc agcaattcaa cgagtccaat ccctgcgggg ttgatcttga tgttgtgaac
      901 atggagtatg aattctgggg aaattgcgcc accgagcttc ccatctttac ggagtacatc
      961 caggcaatgg atgctttccg tgaccaatac gactcccttc ataccgaaca tccgctcatc
     1021 acagaagcgt atgtagcctt tctgacatat tgcaatttcc ccaatgtcgt cgccatcatg
     1081 gacggctgtt ccaattgcag cccgtgtccg acttgcaaca acccgcatga acccctgatc
     1141 gatcgtgttc tctattcaca acagatcatg cagccaaact acttcctgct cgctgatcaa
     1201 agtcattttg aagcccctac cagtcaggat agcaccgact tccatccact cctgtatgcg
     1261 gaaagcattc agaccggtgg tggcgcggat tacctcggcg cctggtttcc gcaatcccct
     1321 tcctttacca tcttcaccgc agaatcccga tactactccg cctggcggaa ttatactaca
     1381 acgccttttg ggcaaccacg acagaacgat gtacaacgcg gcggcgccat gtggttcgct
     1441 tcatcacaca tgatggactg gctcgaccat ccagtgatcc ttcaaaacct cggacccttt
     1501 tgcacgcaaa gtgatactca gcaagtggaa atgaaatatt tcggcccaac cgaaccgggt
     1561 atcggatacc gtttccacat cttccgagac agcgatagca caatcgttta tcctaccaat
     1621 ggcaacccgt tggcgggaat cacccaaccc tacgaaccct cagtgggagg aagtcgcgag
     1681 atcatggcgg ttgatttctc agacacgttg ctctttcctc gcattcgtct tgcacatggc
     1741 acatatacca gtgtgctgga attggactac gactctgcct cgaatgccgg atacacagcc
     1801 cgatatccgg tagtcgtatc cgatcaacca caaatcgtcc tgatgaacga acctgaattc
     1861 tgcgcaggtg gctactgcta cctggtcacc aattccggtg gtagtcaata cacctggaca
     1921 cgtgacaata tcgtcgtgaa atcaggaacc gaatggtggg tgaaagccac agaagacggt
     1981 tattataaat gcgtcatctc aggggcgcca ggaagttgtt ccggcaccac ggactcggta
     2041 cgcatacagg tcatgccaat gccggttgtt gtagtgaatg cgctttgcaa tggagatggc
     2101 accgttacac ttaaaaccga tatgcgtccc gccaacccca actctaccac cacgagcgga
     2161 cccggcggag taacctacaa atggaatacg ggcgcgacca ccgatcaaat caccattacg
     2221 cccacatcca gcaacaccta tcgggtcctg gtgaccgacc cttactccgg ttgcagtact
     2281 tatcgggact gctacgttgc agcccctcca agcaattcgt ttacaccgaa tatcgttgtg
     2341 aacagcgtgc caacatcagc ttgctcatcc aatggctcgc tgaccgcaac cttgtcgccc
     2401 ggaagctatc taggacaaac cgcctatcta tggaataccg gacaaaccac acaaaccatc
     2461 acgaatctac cgcccgggac gtacatcgtt tcgatgagtt actggaaaag cgcctgcacg
     2521 tattacgaca ccgttcaact tggaacacct acgactggcg ggccatccat tagcgaggtg
     2581 attattccat ccggttgttc gaatactgcc agtggaagtg tacaactcat gttgaccggc
     2641 ggccaaccac ccttcagttt cttttggttt gggatacctg cggatggaat ccaccacccc
     2701 gccaaccaag accaatcgaa cctgtttccg ggtatttata caattcgagt ccgcgatgtc
     2761 aatggttgtg aattctggca tgaagtaacg gtaccttccg gcaattcaat aatccgcgtt
     2821 caaccggttt ccattcttcc cagtagcaat tgctttaaca gcccgaatgg gtcggcgact
     2881 gtcagtgcaa gcggcggcac cggtccatat caattcgact ggaatgaccc actggggcaa
     2941 caaggcgcta cggccgacag cctggtagca ggaagttatt gggtcacggt tacggatgcc
     3001 aatggctgca catcttctac cttcatcgat atccccgcca cctcccttcc gatcaacttg
     3061 acagaacttg catcggaatc cctccccgct acgtgtcaac ccggaaacga tggcgtggca
     3121 gcgctcgttg ttcaaggtgg cgtcgtgcca tatactgtta atagtccctg gacgcttgac
     3181 acacttggaa ccgcacgact taccggtacg ccgggaaact atccgatcct tgtcaccgat
     3241 gccaatggtt gtcaattcac agatacgatc actatccaca ctaccgaacc accctcgttc
     3301 tcgctgtcgg cgctcggtac cagttgtagt gggtgtacgg atggcgcggt cgctatcaac
     3361 aatatagtta caaacagtaa ctggaatgtt caatggactc cacagcttgg caccctttct
     3421 ggagacagtc tcattcaaat gcccggtgga
//
LOCUS       DAOTON010000222         3447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21597_length_3447_cov_3.365566, whole genome shotgun sequence.
ACCESSION   DAOTON010000222 DAOTON010000000
VERSION     DAOTON010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21597_length_3447_cov_3.365566"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..182
                     /locus_tag="P5292_05500"
     tRNA            109..182
                     /locus_tag="P5292_05500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:143..145,aa:Arg,seq:tct)
     gene            complement(240..1631)
                     /locus_tag="P5292_05505"
     CDS             complement(240..1631)
                     /locus_tag="P5292_05505"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HRS38618.1"
                     /translation="MSSVDRRNLFGALLVFGFVAVLVSCRKAEPWEEEAFDERLSGGA
                     QTVFTEGVGAFSQAFPTLSGWREEFHELGDQHFEATFVAAPAPLFQGLGTIYNSNSCF
                     NCHINDGRGKPIQQSEPMTSMLFRTSVPGSDPHGGPLAAPGFGGQLQDKANFGVAAEA
                     GVQVMWEETAFVFADGDTVQLRRPVWTLVSPYTGLPAGFMLSPRVAPPVHGLGLLEQI
                     AGSELLALEDESDRDGDGISGHANRVFDAVTRQQTIGRFGWKAEAPNLEQQVAGAYNE
                     DMGITNKVFPVESSYGQPQFDGRSDEPEVPDSIFNSVVFYVKTLAVPARRNVTDPVVR
                     RGKELFNAANCSGCHRTSFTTRTDMYFPEASGQKIHPYTDLLLHDMGPGLSDDRPTYS
                     ASGSEWRTPPLWGIGLTQVVNGHNNFLHDGRARSLMEAILWHGGEAGRSVDFVRNLGK
                     EDREALLAFLKSL"
     gene            complement(1628..2707)
                     /locus_tag="P5292_05510"
     CDS             complement(1628..2707)
                     /locus_tag="P5292_05510"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HRS38619.1"
                     /translation="MKRYFLPAIVLLGLLGSCKKSDDTTPGDSGMTNEQLESAILSDF
                     SSHVARANYNELEQLLDQLHADALALQQTTDATNLTAARSAWRTARAAWEKSEAFLFG
                     PVSTENIDPSTDTWPVDYLSLDSLLSSTLDFTPANIHALGDELKGYHPAEYLLWGTNG
                     DKQAGQFTTRELEFLVALTADLKDKGTALRSSWDASQSGNYGEVFSSAGSGSTVYASR
                     RAAFEELVNGITGICDEVANGKIAEPFLAQDPSLEESPYSNNSLIDFTNNMIGVRNVY
                     RGDFNADGKGLQDYLQANNLSLHNTITAQIDQAIASLNAITVPFGEAIISQPTQVQLA
                     IDQINTLKTTLEDQLLPYLQQNIVR"
     gene            complement(2721..3155)
                     /locus_tag="P5292_05515"
     CDS             complement(2721..3155)
                     /locus_tag="P5292_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HRS38620.1"
                     /translation="MSSRRNFIKTSCLACAQFAGLTAVLASLQSCSSIRILETGISGG
                     SIDVPFSSFEPEEHLKIVRVPQQVYDILLVRQPGQAATALLLQCTHEDTRLVATNSGL
                     YCNLHGSSFSLSGEVKAGPAIRRLKQFPVTEHTDHYTIQFNS"
     gene            complement(3157..>3447)
                     /locus_tag="P5292_05520"
     CDS             complement(3157..>3447)
                     /locus_tag="P5292_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38621.1"
                     /translation="SKKQNKQRQLIVFSRYEGLDQMASVPDNGIRDELYHLQFLIMGL
                     TYQPTGGVSIKFDWKHVTSGEPEPTLNFNPSPTAPGIERVNDFLQLGLAYSF"
BASE COUNT          783 a          961 c          921 g          782 t
ORIGIN      
        1 gcagtaaaca aaacacagaa aattaattgg tcccgtagct caactggata gagcatcccg
       61 gttctaccgg gacggttgag gcagtaaaca aaacacagaa aattaattgg tcccgtagct
      121 caactggata gagcagctgc cttctaagca gccggttggg cgttcgagtc gccccgggat
      181 cacgaatctt cgatcgtttt ttccagtcta attaccagtt actatttact agttccgaat
      241 cacagacttt tcaaaaaagc cagcaacgct tcccgatctt ccttgccaag gtttcgaacg
      301 aaatccaccg atcggcctgc ttcacctccg tgccagagga tggcttccat caggcttctc
      361 gccctaccgt catggaggaa gttattgtgt ccgttcacaa cctgggtgag accgatgccc
      421 cataagggtg gggtcctcca ctcgctaccg gatgcagaat aagtcggccg gtcgtcgctc
      481 agacccggcc ccatatcgtg caacagcaaa tccgtgtaag gatggatctt ttgtccggaa
      541 gcttccggga aatacatatc cgttcgcgta gtgaaactgg tgcgatgaca gccgctgcaa
      601 ttagccgcgt tgaataattc cttcccacga cgaaccaccg gatccgtcac attcctgcgc
      661 gccggcacgg ccagtgtttt tacatagaac accaccgaat tgaaaatgct gtccggtact
      721 tccggctcat cgcttcgtcc gtcgaattga ggctggccat agctgctttc aaccgggaag
      781 accttgttgg tgatccccat gtcttcgttg tatgcaccgg ctacctgttg ttccagattg
      841 ggcgcctccg ctttccaacc gaagcggccg atcgtttgtt gcctggttac cgcgtcaaag
      901 acccggttgg catggcccga tatcccatca ccatcacgat ccgactcgtc ttcaagtgcc
      961 agcaattcac taccggcaat ttgttccagc agtcccaagc cgtggaccgg aggtgcgact
     1021 cttggcgata acatgaaccc tgcaggcaag ccggtatagg gcgataccag tgtccagacc
     1081 ggcctgcgca attgtacggt gtctccgtcg gcaaacacga aagccgtttc ctcccacatg
     1141 acttgtacgc cggcctccgc cgctacgccg aagttcgcct tgtcctgtaa ctgcccgcca
     1201 aaacccggcg cagccagggg accgccgtgc ggatcactgc ccggcacact ggtgcggaac
     1261 aacatgctgg tcatcggctc cgattgctgg atcggctttc cacgaccgtc gttgatgtgg
     1321 cagttgaaac aggaattact gttgtagatg gtacccaatc cctgaaacaa aggagctggc
     1381 gcagcgacaa aagttgcttc gaagtgctgg tcgcccagtt cgtggaactc ttcgcgccat
     1441 ccggaaagcg tgggaaaggc ctgtgaaaat gctcccaccc cttcggtgaa gactgtttgg
     1501 gcgcctccgc tcaaacgctc atcgaacgct tcctcctccc agggctcggc tttccgacaa
     1561 cttacgagaa cggcaacgaa gccgaatacc aggagcgccc cgaataagtt ccgcctgtca
     1621 acgctactca tcgaacgatg ttctgttgca agtacggtaa caactggtcc tccagcgtgg
     1681 tcttgagggt attgatctga tcaatggcca actgaacctg ggtcggttgg gaaatgatcg
     1741 cttcaccgaa cggaaccgtg atcgcattca atgatgcgat cgcctggtcg atctgggccg
     1801 tgatcgtatt gtgcagcgac aggttgttcg cctgcagata atcctgcaac cctttgccgt
     1861 cggcgttgaa atcaccacgg tagacatttc gaacaccgat catgttattg gtgaaatcga
     1921 tcaggctgtt gttgctgtat ggcgattctt ccagactggg atcctgtgcc aggaacggtt
     1981 ccgcgatctt cccgttggcg acttcgtcgc agattccggt gatgccgttc accaattcct
     2041 cgaatgcggc acgtcgggaa gcatagaccg tactgccgct tcccgcactg gaaaagacct
     2101 caccgtaatt acccgattga gaagcgtccc aactggaacg cagggctgtg cctttgtctt
     2161 tcaggtcggc tgtcaaagcg accaggaatt ccaactcacg cgtagtgaac tgacctgcct
     2221 gcttatcgcc attggtaccc cacaacagat actcggccgg atgatagcct ttcaattcat
     2281 cccccaatgc atgaatgttg gccggagtga aatccagcgt ggaggataac aatgaatcga
     2341 gggaaaggta atccaccggc caggtatcgg ttgaaggatc aatattctcg gtagagacag
     2401 gtccgaacag aaaagcttcg ctcttttccc aggcggcccg tgctgtccgc caggcggatc
     2461 gtgcggctgt cagattcgtt gcatccgtgg tctgttgcaa cgccagcgcg tcggcatgca
     2521 gttggtcgag caattgctcc aactcgttgt aattcgcacg agcgacatga ctggaaaaat
     2581 ccgagaggat ggcggactcc agttgttcgt tcgtcatccc ggaatctccg ggtgttgtat
     2641 catcgctttt cttgcaggag cccagcaggc ccagcaaaac gatagcaggg aggaaatatc
     2701 gtttcacggt ttgggggagg ttaggaattg aactgtatcg tataatggtc ggtatgctcg
     2761 gtaacgggga attgcttcag tcggcggatc gccggaccag ccttcacctc accgctcagc
     2821 gaaaaggaac tgccgtgcag gttgcagtac aggcccgagt tggtagccac gaggcgggta
     2881 tcttcatgcg tacactgcaa caagagcgcc gtagcagctt gtccgggttg acggaccagc
     2941 aagatgtcat aaacctgctg gggtacccgt acgatcttca ggtgctcctc cggttcaaag
     3001 gaactgaacg gaacatctat ggaacctccg gaaataccgg tctccagaat gcgaatggac
     3061 gaacacgact gcaggctggc aagcacggcc gtcaggcccg cgaactgcgc gcaggcgaga
     3121 caggaggtct tgatgaagtt ccggcgactg ctcatatcag aaactgtagg ccagcccgag
     3181 ttgcaggaaa tcgttgaccc gttcgattcc cggcgcggtt ggagaaggat taaaattcaa
     3241 ggtaggttcc ggctcgccgg aggtgacgtg cttccaatcg aatttgatgg ataccccacc
     3301 ggtcggctga taggtcaacc ccatgatcag gaattgcaaa tgatatagct catcgcggat
     3361 tccgttatcc ggtacactgg ccatctgatc caaaccttcg tagcgggaaa agacgatcaa
     3421 ctgtcgctgc ttgttttgtt tcttcga
//
LOCUS       DAOTON010000223         3444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21621_length_3444_cov_3.125111, whole genome shotgun sequence.
ACCESSION   DAOTON010000223 DAOTON010000000
VERSION     DAOTON010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21621_length_3444_cov_3.125111"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="P5292_05525"
     CDS             complement(<1..228)
                     /locus_tag="P5292_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRS38622.1"
                     /translation="MEKVFEVHLPQFDGPFDLLLFFIERDEVDIHNIPISKITNDFLE
                     YIRQMNELNIELASEFILVAATLMRIKAKMLL"
     gene            504..863
                     /locus_tag="P5292_05530"
     CDS             504..863
                     /locus_tag="P5292_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HRS38623.1"
                     /translation="MRPYILHLSTCSTCQRILKEWKPGSGVILQDIKNEPLTAKQVDE
                     LKELAGSYEALFSRVARKYRELGLHELELKEADYRKYLLQDYTFLKRPVLVIGRRIFI
                     GNQAKTVAAAKAALKAG"
     gene            860..1759
                     /locus_tag="P5292_05535"
     CDS             860..1759
                     /locus_tag="P5292_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRS38624.1"
                     /translation="MSTRFRSHAAIFAANLIYGANFSIAKIAMPHYIRPAGFILLRVG
                     FALLLFRIVEWATRTGSPVERKDHWRFFLLGLFGVAINQSLFFEGLSRTSNINAALIM
                     TTTPILVMIAASLIIGERITTRRVVGVASGITGAAGLILLGVKASGHASWLGDTMVFT
                     NAVSYAIYMVMAKPMMRKYHPWAVIRWTFFYGFLLVIPLGLKDVASIEWSTFSTPVWL
                     STVYVILFTTFFAYLLNTYGLQHLSPSVVSFYIYFQPLFATGISLLIAQETVSWIQAG
                     ACVLIFLGVYLVAGPVRRKPEVA"
     gene            1804..2679
                     /locus_tag="P5292_05540"
     CDS             1804..2679
                     /locus_tag="P5292_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRS38625.1"
                     /translation="MIRSMTGFGTAAVNLPSKTITVEIKSVNSKFFDLTLRLPAQYRE
                     KELELRAELNRFIERGKVECTIGIESQEAGKRTSVNLPLLKAYDAELQSAQKALGLKQ
                     PDNYFQILLGLPEVLVNERNSLAEEEWNALLGCLQEAMHRFDEFRKNEGKALGKDLSE
                     RIAGIGKGLAALEPYEAGRIEQVRKRLQGSLDEFIEQNNLDRNRFEQELIFYIEKFDI
                     SEEKVRLRSHLDYFIKVLKGEQSSGKKLGFIGQEIGREINTIGSKANDAAMQQVVVGM
                     KDELEKIKEQVLNVL"
     gene            2684..3247
                     /gene="gmk"
                     /locus_tag="P5292_05545"
     CDS             2684..3247
                     /gene="gmk"
                     /locus_tag="P5292_05545"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRS38626.1"
                     /translation="MQGKLLLFCGPSGSGKTTIVRHLLDTDPRLAFSISATTRPKRET
                     ETDGVDYHFISVNEFKRRIDAGEFVEWEEVYKDRFYGTLRSEVDRLWRENKVVVFDVD
                     VEGGLQLKKTFGELLLAVFVMPPSVEALHQRLTARQSETPESLKARVAKAEHELTYAF
                     RFDRVIVNDTLEHALEEAKQVVAEFVG"
BASE COUNT          765 a          966 c          973 g          740 t
ORIGIN      
        1 cagcagcatc ttggccttga tccgcatcag cgtggccgcg accaggatga actcactcgc
       61 caactcgatg ttgagctcgt tcatctggcg gatgtattcc aaaaagtcgt tggtgatctt
      121 ggaaatcggg atgttatgga tgtcgacctc gtcgcgttcg atgaagaaca acaagaggtc
      181 gaacggcccg tcgaattggg gcaggtgtac ttcgaagact ttttccacgg gaaagagggt
      241 ctcgtacaaa gataggaaaa agggactttc cagggttgta tgttccgggt tccgagttcc
      301 gggttccgag ttgagttatt ggttattggt tattagttat tagtaattag tttttattgt
      361 acacactggt tttgaacgaa ataccaaatt ccactttcaa cattcaacat tcaacattca
      421 accttcaact ttcaactaaa aaacaacccg gaacccgaaa cccggaaccc ggaacaatag
      481 gcctaccttc gcaaactatg cccatgcgcc cgtacatcct ccacctctcc acctgctcga
      541 cctgtcagcg gatcctgaaa gaatggaagc cgggaagcgg ggtgattttg caagatatta
      601 aaaacgagcc gcttacggct aagcaggtgg atgaactgaa ggaactggcg gggagttacg
      661 aggctttgtt cagccgggtg gcgcggaagt accgggagct gggtctgcac gagttggaac
      721 taaaggaggc ggattaccgg aagtacctgt tgcaggacta cacctttctc aagcggccgg
      781 tgctggtgat cgggcgacgg atcttcatcg gcaaccaggc caagacggtg gccgcggcca
      841 aagcagcttt gaaggcagga tgagcacccg tttccgctcg cacgcggcga tcttcgcggc
      901 gaacctgatc tacggcgcca atttttccat cgccaagatc gccatgccgc actacatccg
      961 tccggccggt ttcatcctgc tgcgcgtcgg tttcgcctta ctgctgttcc gcatcgtcga
     1021 atgggcgacg cgtacgggca gtccggtcga gcgcaaggac cactggcggt tcttcctgct
     1081 cggcttgttc ggcgtagcca tcaaccaatc cctcttcttc gaaggcttgt cgcgcacctc
     1141 gaacatcaac gcggcgctga tcatgaccac cacgcccatc ctggtgatga tcgcggcttc
     1201 gttgatcatc ggtgaacgca tcaccacgcg gcgggttgtg ggtgttgcca gcggcatcac
     1261 cggtgcggcg ggactcatcc tgctcggtgt gaaggccagc ggtcatgctt cctggctggg
     1321 cgacacgatg gtgttcacga acgcggtctc gtacgcgatc tacatggtga tggccaaacc
     1381 gatgatgcgc aagtaccatc cctgggcggt gatccgctgg acgttctttt acgggttcct
     1441 gctggtgatc ccgctcggac tgaaggacgt cgcgtcgatc gaatggtcca ccttcagcac
     1501 gccggtctgg ttgtcgaccg tgtatgtgat cctcttcacg accttcttcg cttatctgct
     1561 gaacacctac ggcctgcaac atttgagtcc gtcggtggtg agcttctata tttacttcca
     1621 gccgctcttc gccacgggta tttcgttgct catcgcgcag gaaacggtga gttggatcca
     1681 ggcgggggcc tgcgtgctca tcttcctcgg cgtttacctc gttgccggtc cggtccgcag
     1741 aaagcccgaa gtggcttgat attcacgggc atcggcgtac ttttgcagcc tcacaccccg
     1801 cccatgatcc gttccatgac cggcttcggc accgccgcgg tcaacctgcc ctccaagacc
     1861 atcacggtgg agatcaagtc ggtgaacagc aagttcttcg acctgaccct ccgcctgccc
     1921 gcgcaatacc gggaaaagga actcgagctg cgcgccgagc tgaaccgttt tatcgaacgc
     1981 ggcaaggtgg aatgcacgat cgggatcgag tcgcaggaag cgggtaagcg tacttccgtg
     2041 aacctgccgt tgctcaaggc gtatgacgcg gagttgcagt cggcccaaaa ggcgctcggc
     2101 ctgaagcagc cggataatta tttccagatc ctgctcggcc tgcccgaggt gctggtgaac
     2161 gaacgaaaca gtctggcgga agaagaatgg aacgcgctct tgggttgttt gcaggaagcg
     2221 atgcatcgtt tcgacgaatt ccggaagaac gaagggaagg ctttgggcaa ggacctgagt
     2281 gaacgcatcg ccggtatcgg aaaaggattg gcggccttgg agccttacga agcaggccgc
     2341 atcgaacagg tgcgcaaacg gctgcaaggc tcgctcgacg agttcatcga gcagaacaac
     2401 ctcgaccgca accgcttcga gcaggaactc atcttctaca tcgagaagtt cgacatctcc
     2461 gaggagaaag tccgcctccg ctcccacctc gactatttta tcaaggtgct gaaaggcgaa
     2521 caatcctcgg gcaagaagct gggcttcatc ggtcaggaga tcggtcggga gatcaacacg
     2581 atcggttcca aggcgaacga cgcggcgatg caacaggtgg tggtgggaat gaaagacgaa
     2641 ctggaaaaga tcaaagaaca ggtactcaac gtactctgat cccatgcaag gaaaactcct
     2701 gctcttctgc ggaccctcgg gcagcggcaa gacgaccatc gtccgccacc tgctcgatac
     2761 cgatccgcgc ctggccttct ccatctcggc cacgacgcga cccaagcggg agaccgagac
     2821 cgatggcgtg gactaccact tcatcagcgt aaacgaattc aaacgccgca tcgacgcggg
     2881 cgagttcgtc gaatgggagg aagtttacaa ggaccgcttc tacggcacgc tgcgttcgga
     2941 ggtggaccga ctgtggcggg agaataaagt agtggtgttc gacgtggacg tcgaaggcgg
     3001 gttgcaactc aagaagacct tcggggaatt gctcctcgcg gttttcgtga tgccgccctc
     3061 cgtggaagcc ttgcaccagc gcctcaccgc gcgtcagtcg gagacccccg aatcgctcaa
     3121 agcccgtgtc gccaaagccg agcacgaact cacctacgcc ttccgcttcg accgtgtgat
     3181 cgtgaacgac accctggagc atgcgctgga ggaagcgaag caggtagtgg cggagtttgt
     3241 gggttgagga tttccgggtt cacgttccga gttccgagtt ccgagtttcg agttgaagta
     3301 caaacgtcaa atagcgacaa tcgctaataa caaatatcaa atcactaatt actagttact
     3361 agttactaat taccagttac cagtttccct ccccccctcc atgaaaatcg gcctctactt
     3421 cggctccttt aacccggtcc ataa
//
LOCUS       DAOTON010000224         3437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21678_length_3437_cov_3.412478, whole genome shotgun sequence.
ACCESSION   DAOTON010000224 DAOTON010000000
VERSION     DAOTON010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21678_length_3437_cov_3.412478"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="P5292_05550"
     CDS             complement(<1..505)
                     /locus_tag="P5292_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38627.1"
                     /translation="MRIFRYSLLALLFLLVSPAVFGQGLRNFTDDPVKFADELQKFLA
                     ETDKKEAEKVMEAFLPYWTGGKFSPQQQTAIYKTCNAMLRKRMKAIPDFRNYVMTLTS
                     FAASNQSAESFAAWQSSIDKLLLMPTRFFPQYINTCNLLFRDNTLYESASTRWHTQHN
                     NYRFEFDS"
     gene            complement(502..1329)
                     /gene="prmA"
                     /locus_tag="P5292_05555"
     CDS             complement(502..1329)
                     /gene="prmA"
                     /locus_tag="P5292_05555"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRS38628.1"
                     /translation="MSWIKVSMQAAGIDPEVVLAWAAELDFDAFEQEGSILNAYVPAD
                     RFDEAAFGRLASEFPFGENLRWTASLLEDKNWNETWEKQYEPVRIDQRVTIRAPFHPA
                     APEGDIALEIEPKMSFGTGHHPTTTLMIRMMLRESFIGKTVLDLGCGSGVLGILAARL
                     GAVEVTGIDIDVWAVENTRENCERNGITCMRVEQGDARAIGERQFDFVLANINRNILV
                     KEMPVMANAMHPNALLMMSGFLNQDLPVIREAAAASGLHFIYTETDNEWAAITFQKP"
     gene            complement(1334..2101)
                     /gene="tpiA"
                     /locus_tag="P5292_05560"
     CDS             complement(1334..2101)
                     /gene="tpiA"
                     /locus_tag="P5292_05560"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRS38629.1"
                     /translation="MRTPLVAGNWKMNLQYAEAMTLVDAIVDGLPENNGAEIVLAPPT
                     IYLHDVLDRIQRKPGIALSAQDVSAYERGAFTGEVAASMLASVGVEYVIVGHSERREY
                     HHEDHPLLAAKVDRVLEQSLLPIFCCGEKLDDRTAGRHFELIEQQLHESLFHLSPEKV
                     TECVIAYEPVWAIGTGVVATTGQAQEMHRFIRQLLFARYGEPIASEMRILYGGSVNAT
                     NASDLFTCPDVDGGPVGGASLKAVDFLSIIAAAGTNR"
     gene            complement(2143..3219)
                     /locus_tag="P5292_05565"
     CDS             complement(2143..3219)
                     /locus_tag="P5292_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS38630.1"
                     /translation="MLNFLFRRLWNGLLVVAGVAVFVFLLFSVLPADPARMMLGQQAD
                     SASLAIINRDLGRDLSVSRQFVVFINDLSPLSLHSTDTVSRYYAHKEKYGSVVHLLRL
                     GKTELVAKLPYLRKSYQNKRPVSEILLEKLPETGILALASILLATFLGIILGLVAALN
                     KDRWPDRLALAFSVAGVSLPSFFAAILIAWWLGYAWSDWTGLEMTGSLREIDPFHGEY
                     IAWKNLLLPTLVLGLRPMAIITQLTRSSLLEVLSLDYIRTARAKGVSSRRVLLRHALR
                     NALNPIVTAVSGWLGSLLAGAVFVEYIFGWNGIGKLTVHALEQYDLPLVMGVVLFISV
                     IFVILNLIADLINAWLDPRIRVGG"
BASE COUNT          877 a          917 c          892 g          751 t
ORIGIN      
        1 tggagtcgaa ttcgaagcgg taattgttat gctgagtatg ccaacgggtg gaagcggact
       61 catacaaggt gttatcccgg aaaagcaggt tacaggtatt gatgtactgg ggaaaaaaac
      121 gtgtaggcat caacaacagc ttatcgatgc tggattgcca ggccgcgaaa ctttccgctg
      181 actgattgct tgcggcaaaa ctggtgaggg tcatcacgta attcctgaaa tccggaatgg
      241 ctttcatccg tttccgaagc atcgcgttac aggtcttgta gatggcggtt tgttgctgcg
      301 gggagaactt accaccggtc cagtatggga gaaaagcttc catcactttc tccgcttcct
      361 tcttgtcggt ttcagccagg aacttttgca actcatccgc aaacttcaca ggatcatcgg
      421 tgaagttgcg caacccttgt ccgaatacag ccggcgaaac cagcaggaac agcagcgcta
      481 aaagtgagta tcgaaaaatc ctcatggttt ttggaaggtt atggcagccc attcgttatc
      541 ggtttcagtg tagatgaagt gaagtccgga agcggcagct gcttcccgga tgaccggaag
      601 atcctggttc aggaatccgc tcatcatcag gagagcattc ggatgcatgg catttgccat
      661 gaccggcatt tccttgacca gtatattccg gttgatgttg gcgaggacga agtcgaattg
      721 cctttcaccg atcgcgcgtg catctccttg ctccactcgc atgcaagtga tcccgttgcg
      781 ctcgcagttt tcccgggtat tctccacggc ccaaacatca atatcgattc ccgtcacctc
      841 gacggcaccc agtcgggctg ccaggatgcc caacactccg cttccacagc cgaggtccag
      901 gaccgttttc ccaatgaacg attcgcgcag catcatgcga atcatcaggg tggtggttgg
      961 atgatgaccg gttccgaatg acatcttcgg ctcgatctcc aaagcaatat cgccttccgg
     1021 tgcggctgga tgaaaggggg cgcggatggt cacgcgctgg tcgattcgta ccggttcata
     1081 ctgcttttcc caggtttcat tccagttctt gtcttccaat aacgaagcgg tccagcgcaa
     1141 attttcgccg aatggaaatt cacttgccag gcgaccgaat gcggcttcat cgaatcggtc
     1201 agccggaacg taagcgttga ggatgcttcc ctcctgctcg aaagcatcga aatccagctc
     1261 ggcggcccag gccagtacca cttccgggtc gatacccgca gcctgcatgg aaactttgat
     1321 ccagctcatg cgatcagcgg ttcgtgccag cggctgcgat gatggaaagg aaatcgacag
     1381 ctttcaggga cgcgccgcct accggtccgc cgtccacatc cgggcaagta aacagatcgc
     1441 ttgcattggt agcatttacg ctcccgccgt agaggatccg catttcggat gcaatcggtt
     1501 cgccataccg ggcaaaaagc aattgtcgaa tgaagcggtg catctcctgc gcctggcctg
     1561 tcgtggcgac caccccggta ccgatcgccc aaaccggttc ataagcgatc acacattctg
     1621 tcactttctc aggagaaaga tgaaagaggc tctcgtgcaa ttgctgttcg atcaattcaa
     1681 aatgtcggcc tgcagtacgg tcatccagtt tttctccaca acagaaaatg ggcaacagcg
     1741 attgctccag cacgcgatcg acctttgccg ccagcaaagg gtgatcctcg tgatgatatt
     1801 cccgtcgttc gctatgtcca acgatcacat actcgacacc caccgaagcc agcatggaag
     1861 ccgctacttc accggtaaaa gcgccacgtt cgtaggcgga gacatcctga gccgacagtg
     1921 cgataccggg ctttcgctga atgcggtcca gcacatcgtg caggtagata gtcggcggtg
     1981 ccagcacgat ttccgcgccg ttgttttccg gcaagccatc aacgatcgca tcgaccaggg
     2041 tcatcgcttc ggcatattgc aggttcattt tccagttgcc tgcgacgagt ggagttctca
     2101 tgattcttca ggaggtagat attcgaggat aaaaatagga cattatccgc cgacgcggat
     2161 gcgcgggtcc aaccaggcgt tgatcaggtc ggcgatcagg ttcaatatga caaagatgac
     2221 ggagatgaaa agcactacgc ccatcacgag tggcaggtca tactgctcca aagcatgtac
     2281 cgtaagctta ccgataccgt tccaaccgaa gatatattcc acaaaaacgg cgcctgccag
     2341 caaggagccc agccagccgc tcacggcggt tacgatcggg ttgagcgcgt tacgaagcgc
     2401 atgtcgcaac agaacacggc gggaggatac acccttcgcg cgggccgtac gaatatagtc
     2461 gagcgacaag acctccagca gggaactacg cgtaagttga gtgatgatcg ccatcggacg
     2521 tagaccaagc accagggtgg gcaacagcag gttcttccag gcgatgtatt caccatggaa
     2581 cgggtcgatc tcgcgtaaac tcccggtcat ctccaatccg gtccagtcgc tccaggcata
     2641 acccaaccac caggcaatta ggatagcggc aaagaagctc ggcaacgaaa cacccgcgac
     2701 agaaaaagcg agcgccaacc gatccggcca acgatccttg ttcaaggctg ctaccagtcc
     2761 gagtataatt cctaaaaagg tcgcgagtaa aatggatgca agcgccagta tcccggtctc
     2821 aggtaatttc tccagtagga tttccgaaac gggacgtttg ttctgatagg acttacgcag
     2881 ataaggcaac ttcgcaacaa gttcggtctt cccgagccga agcaggtgaa cgaccgaacc
     2941 gtacttctcc ttatgagcat aataacggct gaccgtatcg gtcgaatgca aggagagtgg
     3001 cgagaggtca ttaatgaaga caacgaattg ccgggaaacg gaaagatcac gtccgaggtc
     3061 gcggttgatg atcgcaagcg aggctgaatc cgcctgctga cccaacatca tccttgccgg
     3121 gtcagcgggg aggacggaaa atagcagaaa gacgaataca gctacaccgg ctacgacaag
     3181 caagccgttc cataaacgcc ggaagaggaa attcagcatg gtacgaattg cagaattaag
     3241 attttatcag gccaggcaag ccggccaaac gttgcatttt gtaacatact ttcaaacaaa
     3301 aaaccgattt taaaattgct gcttctgact gaattgcaac agcttagatc attttagaaa
     3361 ataacctaca tttggaaaca accattctcc gcagacacaa tgcaaaggaa tttcaagtta
     3421 ctgattatct gcatttt
//
LOCUS       DAOTON010000225         3435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21689_length_3435_cov_4.000296, whole genome shotgun sequence.
ACCESSION   DAOTON010000225 DAOTON010000000
VERSION     DAOTON010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21689_length_3435_cov_4.000296"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..726
                     /locus_tag="P5292_05570"
     CDS             94..726
                     /locus_tag="P5292_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38631.1"
                     /translation="MKILALVLMVLTILVIVGFGFAYLLGFVMSFDAPGSDKDPDAWS
                     FRILMLGPIIIFLSLFVLSIRAYSAGQYKRSVLMGAATPVIGLGLFGAMTLSSVASYK
                     SYQKEEARQRELEKRYPVQRFTRPAEGGTDTLIVWPSGIVAYRLYMGPDKPVWGGPFG
                     DLSDDRRTILYKASKDNKLKPEDLDQFKDETGRNLTEVYTVINKTTGGDF"
     gene            complement(773..2482)
                     /locus_tag="P5292_05575"
     CDS             complement(773..2482)
                     /locus_tag="P5292_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38632.1"
                     /translation="MISIIEDNLVEEIRKRYCEQMGENYFPISKKEPDYRARRITEYL
                     NLMGRIVDRQVTALRSDTFENGSDLVKYFEMLPDGVPVRIRYEQMLREKDPANRIQLQ
                     EELRALIRPGSIDVNIMTKCDRINYDRNGEPLPAEFADAMAALRGFANSDLESAVIFS
                     AGMNPRLYSYCESFPDFLPDASGLIRKKIILKVSDFRSALIQGKFLAKRGLWVSEFRV
                     ESGLNCGGHAFPTDGYLLGPILEEFRSRRHELITELETLYLSALKGRQLPLPLEIPSI
                     RITAQGGIGTFAEQQFLLEHYDLYATGWGSPFLLVPEVTNVDPYTLGQLSTASPEDYY
                     LSNASPLGIAFHNFRKSSGNAERNERIRKGRPGSPCKKKFLAFNTEFEGIPLCTSSRA
                     YQEKKLGKIRKELPDGDQRVRAEHIVLEKECLCEGLAASAMIADQSSKPSKDPAVSVC
                     PGPNLAYFSGTFTLREMTDHIYGRGNIRNGLYRPSVFINELKLYVNHFRKEVREVQAI
                     WSDRKQSGLLTFRENLANGIDYYRELLIKVKFDSEAAIENMKMELSQIREELFAISLP
                     VVQ"
     gene            complement(2899..3303)
                     /locus_tag="P5292_05580"
     CDS             complement(2899..3303)
                     /locus_tag="P5292_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRS38633.1"
                     /translation="MANFRSIEFILYVSDQERSKTFYKTLLQSEPVLDVIGMTEFALS
                     DSCKLGLMPNKGIARIIGETLPHPESGTGIPRCELYLIVEDAREVFKHAMHCGARLVS
                     PVADRDWGDRVGYVADPDGHVIALAQYIKTGG"
BASE COUNT          903 a          886 c          757 g          889 t
ORIGIN      
        1 ccgcatccaa gataataaaa ggagctgtac cggattcctt acgggaactg gattttgttg
       61 gtacatttcg ggagtttcaa agttcttccc ggcatgaaaa tcctggctct tgtactcatg
      121 gttttgacca tactggtcat cgtcggtttc ggattcgcct atttgctcgg attcgtgatg
      181 tccttcgacg ccccgggctc cgacaaagac ccggacgcct ggagctttcg gatcctgatg
      241 ctggggccca tcatcatttt tctttcgctg tttgtcctgt ccatccgcgc ctactcagcc
      301 ggccagtaca agcgctccgt cctgatggga gctgccacac cggtgatcgg acttggattg
      361 ttcggagcca tgaccctttc gtctgtcgct tcttataaat cttaccagaa agaagaagca
      421 cgacaacgtg aactggaaaa gcgttatccg gtacaacgct ttacgcgccc agcggaaggc
      481 gggaccgaca ccctgatcgt atggcccagc ggaatcgtgg cttaccgcct ctatatggga
      541 cccgacaaac ccgtttgggg aggaccgttc ggtgacctga gcgatgatcg ccgtactatt
      601 ctttacaagg cgagtaagga taataagtta aagccggaag atctggatca attcaaagat
      661 gaaaccggcc gcaacctgac tgaagtttat actgtcatca ataaaacaac aggaggggat
      721 ttctaagtag tacttgctat caggtgcaaa tctccgtttg catagccggt atctattgaa
      781 cgacaggcaa agaaattgca aataattctt cccgtatttg cgataactcc attttcatat
      841 tttcaatagc agcttccgaa tcaaatttga cttttataag caattcgcga taataatcta
      901 ttccgttagc gaggttctca cgaaatgtca aaagacctga ctgcttacgg tcagaccaaa
      961 ttgcttgtac ttccctcact tccttgcgaa aatgattcac gtacaatttc aactcattta
     1021 taaataccga gggtcgatac aagccattcc gaatattccc tcgtccgtag atatgatcag
     1081 tcatctctcg taaagtgaaa gtcccggaaa aataagccag gttcgggccc ggacaaaccg
     1141 atactgcggg gtccttgctc ggttttgacg attgatcagc aatcattgcc gacgccgcca
     1201 gcccttcgca caggcactct ttttccaaaa ctatatgctc ggcccttacc cgttggtcac
     1261 catctggtaa ctcttttcgg attttcccta gctttttctc ctggtaggct cgggatgaag
     1321 tacataaggg tatgccttca aactcagtat taaacgcaag aaatttcttc ttacaaggac
     1381 taccgggtcg acccttccga atccgctcat tccgttctgc atttccactg ctctttcgga
     1441 aattatggaa ggctattccc aagggggaag cgttacttaa ataataatct tccggcgagg
     1501 cggtgctgag ttgacctaag gtatagggat ccacgtttgt cacctccggg accaagagaa
     1561 acgggcttcc ccagcctgta gcataaagat cataatgctc caataggaac tgttgctctg
     1621 caaaggttcc gatccctcct tgtgccgtaa tacggatcga tggtatttca agaggcaagg
     1681 gaagttgtcg acccttcaaa gcggaaagat aaagcgtttc cagttccgtg atcagctcgt
     1741 gacggcggga gcggaattcc tcgagaatgg gccctaacag atagccgtca gttgggaatg
     1801 catgaccacc gcaattcaat ccgctttcta ctcgaaactc cgatacccag agtcctcgct
     1861 tcgctaaaaa tttaccctga ataagggctg aacgaaaatc actgaccttt aatattattt
     1921 tctttcgtat caaccccgat gcatcgggta ggaagtcggg aaatgattca caataggaat
     1981 aaagtcgagg attcatccct gcagaaaaaa tgacagcaga ttccagatca ctgtttgcaa
     2041 aacctcgtaa ggctgccatt gcgtccgcaa attctgccgg gaggggctcc ccattcctat
     2101 cataattgat acgatcacac tttgtcatga tgttcacatc aatactccca ggacggatca
     2161 acgcacgcaa ttcctcctgc aactgaatcc tgttggcagg atccttttcc cgcagcatct
     2221 gctcataccg tatccgaacc ggaacaccat ccggtaacat ctcaaaatat ttcaccaggt
     2281 cactgccatt ttcaaaagtg tctgatcgaa gagcggtgac ttgccggtca acgatacgac
     2341 ccatcaaatt caggtattcg gtaatcctcc ttgcccgata atcaggctcc tttttggaaa
     2401 tagggaaata attctccccc atttgttcgc aataacgttt ccggatctct tcgacaagat
     2461 tgtcctcaat gatggatatc acggaagaga ttccatatcg tgcgactttt gctggggtat
     2521 cgatagtata cccaagcccc attacgggaa tgtgaaacgt gtgaccggaa ggatacatgg
     2581 aaggggatta gccggaaccg aagtagctac agcaataacc gtcaaaggag gagactatct
     2641 actactttaa ccatgacacg cgtcatcatt tctgctccga aaacgtttgt gtcagatcag
     2701 ttactgtagc actgcagcta gaaaaaccta atcttttatt ctctttaatg atatcatctg
     2761 ccgccattgc ctggatagtg aaaggaacct attagctgcg gatcagtaag ctgagtacaa
     2821 gtgcaaatag taattcgttg agcaagcttt aaatcctgcc actgcagtga acgtacatac
     2881 tccgtaatcc gattaccttc aacccccagt cttgatatac tgtgccaggg cgatcacatg
     2941 tccgtccggg tccgcgacgt aacccacgcg atcgccccaa tcgcgatcgg caaccggact
     3001 gactaatcgt gctccgcagt gcatcgcgtg tttaaaaacc tcccgtgcat cttccacgat
     3061 cagataaagt tcacagcggg gaatacccgt tccgctctcc ggatgcggca aggtttcgcc
     3121 aatgatcctg gcaattcctt tgttcggcat cagtccaagc ttacaactat ccgacagcgc
     3181 gaactcggtc atgccgatca cgtccaggac aggttcggat tgaagcaatg tcttatagaa
     3241 ggttttgctc ctttcctgat cactcacgta aagtatgaac tcaatacttc ggaagttcgc
     3301 catgatatca tacgattgaa tacatattcg ccaacgatta tcggtacagc cacttccgca
     3361 ataaccgtgt ataaggcggc atcaccacat agaccatgag cgagcagatc acaccggata
     3421 cgagcaggtt gtcga
//
LOCUS       DAOTON010000226         3435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21692_length_3435_cov_2.977219, whole genome shotgun sequence.
ACCESSION   DAOTON010000226 DAOTON010000000
VERSION     DAOTON010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21692_length_3435_cov_2.977219"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="P5292_05585"
     CDS             <1..711
                     /locus_tag="P5292_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38634.1"
                     /translation="SCKTSIFNSAIAGFPTGLLVDGANCEANATNNELQYQHNVLAGC
                     ATPLAVASGSSWDISAWFTTPAYGNEQLANSSDLQLMDPNNYANPDFMPSAGSPLLSG
                     ADFSSNYLNDPFFESVSYRGAFGNSDWTNGWANYDCENTNYTVGISEINSIRSISAYP
                     NPAHRTAFIAFSLEQATELTVSVIDLEGREVLTVQPERYSAGEHRIELNVEALAQGIY
                     TLVMRNESAITTLRLAVN"
     assembly_gap    848..857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            878..>3435
                     /locus_tag="P5292_05590"
     CDS             878..>3435
                     /locus_tag="P5292_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS38635.1"
                     /translation="MKHLYCLLLLTFGLNLLVLGNDDGLSGRVTGVVIDKANGETLIG
                     VPVMIEGTTLGAVTDLDGRYLITNIPNGTYNIVFRYIGYTTKIIRNVAVQEGSPINLD
                     VSLENSSQQLNEVVVTADMKRESVGSIILMQKKSATVQDGISAETIRKTPDKNTGEVV
                     RRISGASIQEGRFVIIRGLNERYNGALLNGVPLASSEPDRKAFSFDLFPANMLDNLII
                     VKTASPELPGEFSGGLLQLNTRDIPDQNFFSFSAGTGLNTISTFKKYRSYQGGKTDWL
                     GFDDGTRALPSDFPQAETLQRATQADKIKYSKLLPNDWATEEKASTPLTQTYQASIGR
                     TGKLLRNDFGLIASISYSNGRRTQEFERADYDFDGSQNFDFQDQQYRENVFWGALLNA
                     SYKIGDRHKISFKNLYSDNAEDMTIQREGIDIENQQYINAFALRYSSTRFLNSILSGE
                     HALSAGGTRLKWNDSYTEIRQDVPDLRRMYYYRNLDPSLEDTTLYAYVPFGTASPNYA
                     GKFYSDLGEKVYNGDFSLQTPLKFLGENQTVKFGFFEQYKSRSFDARVLGYVITNAGQ
                     FNWNLLQSPIETLFNPENMGPRGFRIDEITNPSDAYTASTNLHAGFIQFDNLVHKNIR
                     LVWGVRVENFIQKLNSFGYSNDTIRVRENYTDLLPSINVSWSLSEKTNLRIAASQTVA
                     RPEFRELAPFSFYDFSTASTIQGNDTLVRTRIKNFDLRYEYYPAPGEILSASVFYKDF
                     TNPIEPVVESSGAGSRRVSFWNAGSAYAYGLELEWRKKLEFVDGLLSWNQWENFSLYG
                     NVSLIRSRVDVSNDPRATGPRPLQGQSPYVLNGGLQYQNPRSGLGVNVVYNRIG"
BASE COUNT          832 a          985 c          852 g          756 t
ORIGIN      
        1 tcctgcaaga cgagcatctt caacagcgcc atcgcgggtt tcccgaccgg gctcctggtt
       61 gacggtgcca actgcgaagc caacgccacc aacaatgagc tccagtacca acataacgta
      121 ctggccggtt gtgcaacgcc gcttgccgtc gccagtggtt cttcctggga catcagtgcc
      181 tggttcacta ctcccgcata tggtaatgaa caactcgcca acagttccga cctgcaattg
      241 atggatccga acaactacgc caatcccgac ttcatgcctt cggccggctc tcccctcctt
      301 tcgggagcgg acttctcctc gaactacctg aacgatccgt tcttcgaaag tgtcagctac
      361 cgcggcgcgt ttggaaacag cgactggaca aacggttggg ccaactacga ctgcgagaat
      421 accaactaca cggtaggaat cagcgagatc aactcgattc gctccatctc cgcctacccg
      481 aatccggctc accggaccgc tttcattgca ttctcgctcg agcaggctac cgaacttacg
      541 gtatccgtca tcgatctgga aggtcgcgaa gtcctgacgg tacaaccgga acgctattcg
      601 gcaggagaac atcgcatcga attgaacgtg gaggcgcttg cccagggcat ctacaccctc
      661 gtcatgcgca acgaatcagc gatcacgacg ctgcgccttg cggtcaattg atccgcacat
      721 aaaggcaaaa cagacaggcc ggcccatcag ctggtctgtc tgtttatatg gtgtttacga
      781 tacgttcaca aatccttgca tgcttcatgt tgcattaaca taggcttaaa caggtcgtaa
      841 ggtttccnnn nnnnnnntac aaccgatagc aaagcacatg aaacacctct attgcctcct
      901 actattgaca ttcggcctga acctgctcgt actcggcaac gacgatggtt tgagtggacg
      961 tgtgaccggt gtcgtgatcg acaaagcgaa cggagaaacg ctgatcggtg tcccggtcat
     1021 gatcgaaggt actaccctgg gcgctgttac ggacctggac ggacgttacc tgatcaccaa
     1081 cattcccaat ggcacctata acatcgtgtt tcgctatatc ggttatacca cgaagatcat
     1141 ccgtaacgtt gcggttcagg agggatctcc gataaatctt gacgtcagcc tggagaactc
     1201 cagccaacag ctcaacgagg tggtcgtcac cgctgatatg aaaagggaaa gcgtgggatc
     1261 gatcatcctg atgcagaaga agagcgcgac ggtgcaggac ggcatatccg cagaaaccat
     1321 ccgtaaaaca ccggataaga ataccggcga agtcgttcgt cgcatcagtg gcgccagcat
     1381 ccaggaaggc cgtttcgtga tcatccgcgg attgaatgag cgttacaatg gggccttgtt
     1441 gaacggtgtg ccactggcaa gttccgagcc cgaccggaag gcattctcgt tcgatctttt
     1501 cccggccaac atgctggata atctcattat cgtcaaaaca gcatcgccgg aacttccggg
     1561 tgaattctcc ggcggtctct tacaactcaa cacccgcgat attccggatc agaatttctt
     1621 cagcttctcc gcaggcaccg gcctgaacac gatctcgacg ttcaagaagt atcgttcgta
     1681 tcaaggcggc aaaaccgact ggttgggctt tgatgacggc actcgcgcgt tgccttcgga
     1741 ttttccccag gctgaaacgc tacagcgcgc cacccaggcc gacaaaatca agtacagtaa
     1801 actcctgccc aacgattggg cgaccgagga aaaagcttcc actccgctta cccagaccta
     1861 tcaggcatcc atcgggcgta ccgggaaact tcttcggaac gactttggtc tcatcgcttc
     1921 catcagctac agcaacggcc ggagaacaca ggaattcgag cgtgctgact acgatttcga
     1981 tggttcgcag aatttcgatt tccaggatca gcaataccgg gagaatgttt tctggggcgc
     2041 cttgctgaat gcctcgtata agatcggcga tcgtcataag atcagcttca agaatctcta
     2101 ctctgacaac gcggaagaca tgaccatcca gcgtgaagga atcgatatcg agaaccaaca
     2161 atacatcaac gcattcgcgc tgcgttattc atcgacacgc ttcctgaatt cgatcctgtc
     2221 cggtgaacat gcgctctccg caggtggaac ccgtctgaag tggaacgaca gctacactga
     2281 gatacgacag gacgttcccg acctgcgtcg aatgtactac taccggaatc tcgatccgtc
     2341 actcgaggat acaaccctct atgcctatgt cccattcgga acggcatccc ccaactatgc
     2401 cggaaagttc tattcggatc tgggcgaaaa ggtttacaac ggcgacttca gtctgcagac
     2461 acctctcaaa ttcctcggtg aaaaccagac cgtcaagttc ggcttctttg aacaatacaa
     2521 atcccgctcc ttcgatgccc gcgtactcgg ttatgtcatc accaatgccg gtcaattcaa
     2581 ctggaacctg ttgcagtcac ccatcgaaac actgttcaat ccggagaata tgggtccacg
     2641 gggtttccgg atcgatgaga tcaccaatcc ttccgacgcg tatacagcca gcacgaatct
     2701 gcatgccggg ttcatccagt tcgacaatct cgtgcacaag aacatccggc tcgtgtgggg
     2761 cgttcgggtc gaaaacttca tccagaagct gaactccttc ggttacagta atgataccat
     2821 tcgggtacgc gaaaactaca ccgacctgct gccgtccatc aacgtatcct ggtccctttc
     2881 cgagaagacc aatctgcgaa ttgcagcttc gcaaaccgtc gcacgtcctg agttccggga
     2941 gctggcgcct tttagcttct atgatttctc caccgccagt acgatccagg gaaatgacac
     3001 gctggtgcgc acccgcatca agaatttcga cctgcgctac gagtactacc ctgcaccggg
     3061 tgaaattctc agcgcatcgg tcttttataa ggacttcacg aatccgatcg agcccgttgt
     3121 agaaagttca ggcgccggtt cccgtcgggt cagtttctgg aatgcgggca gcgcttacgc
     3181 gtatggcctg gagctggaat ggagaaagaa actggaattc gtcgacggac tgctgtcgtg
     3241 gaaccagtgg gaaaacttca gcttgtacgg taacgtttcg ctgatacgtt ctcgggtgga
     3301 tgtctccaac gatcccaggg ctaccggtcc gagaccactg caagggcaat cgccctatgt
     3361 gctcaacggg ggcttgcaat accagaatcc gcgcagcgga cttggggtga atgtggtgta
     3421 caaccgcatc ggcag
//
LOCUS       DAOTON010000227         3434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21704_length_3434_cov_3.071323, whole genome shotgun sequence.
ACCESSION   DAOTON010000227 DAOTON010000000
VERSION     DAOTON010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21704_length_3434_cov_3.071323"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="P5292_05595"
     CDS             complement(<1..1104)
                     /locus_tag="P5292_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRS38636.1"
                     /translation="MSFKIISEFRPTGDQPEAIRQLVEGLRRGDHAQTLLGVTGSGKT
                     FTVANVIEQVQRPTIILSHNKTLAAQLYGEFKQFFPENAVEYFVSYYDYYQPEAFIPS
                     TNTYIEKDLAINDEIEKLRLSTTTSLLSGRQDVLVVSSVSCIYGIGNPGDFKANTIRV
                     REGEKISRNAFLHKLVTSLYSRTDTDFDRGKFRVRGDTVDVFIAYGDIAYRFYFFGDE
                     IEEIASIDPINGKTLEKHNAVAIYPANIFVTNPERQLQAIWQIQEDMMKQVEYMKSLG
                     QHLEAKRLEDRVTYDLEMIRELGYCSGIENYSRYFDGRDPGQRPFCLLDYFPEDYLLV
                     IDESHVTVPQIRAMYGGDRSRKQNLVEYGFRLPA"
     gene            complement(1204..1962)
                     /locus_tag="P5292_05600"
     CDS             complement(1204..1962)
                     /locus_tag="P5292_05600"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRS38637.1"
                     /translation="MKRFGLFGHPLEHSYSKKFFTEKFQREGRTDCVYENFDSDNIYD
                     LKKIVRDNPDLVGLNVTIPYKQDVIPMLDNLDDISQRIGAVNTIRIQRFENGKFELHG
                     FNTDAWGFELAIRPILRPHHRRALILGTGGSAKAVSYVFRKLAIEHFFVTREESRFHY
                     CYADLNQNTMKAFQVVVNTTPLGMYPNVNDAPNIPYEFLTHKHLCFDLIYNPPQTRFL
                     EKSKASGAFIHNGFRMLRLQAEKAWEIWNEPIYV"
     assembly_gap    2341..2350
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2351..3367
                     /locus_tag="P5292_05605"
     CDS             <2351..3367
                     /locus_tag="P5292_05605"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HRS38638.1"
                     /translation="GTVRRLLPNSRAEVIDSDGFTHIIELHQLVRIEFGLDEQLTVPA
                     EASSTSRPVEKLVEATQAATAKSADTWIRFMDQDQTVYVATALKRPDAPLVSDIDIRL
                     INNTHWTILFTVARKMPDVRSGVRSGILQSRKEESLGVFSADELHRIEGFEFQILFFG
                     EREYAFRPPIVRSLSISPSDFLEPSFRDKLPGHPETVLLHSLAEVMPKGEVDVSRLLE
                     KFSKSEAEAKERQGGAKPARGSKFTILSRERIVDLHIEELIKDPTGMSNAQIISYQLN
                     HFQKELDRALLDKLHKITFIHGVGSGVLRSSIREELKKYPAIRFQDAPPEQFGYGATE
                     VIFQ"
BASE COUNT          845 a          845 c          900 g          834 t
ORIGIN      
        1 ggctggtaag cggaaaccgt actcgacgag gttttgcttg cgtgagcggt ccccaccgta
       61 catggcccgg atctggggta ccgttacatg gctttcgtcg atcaccagca aataatcttc
      121 cggaaaataa tccagcaggc agaagggacg ctgtccggga tcccggccat cgaaataacg
      181 cgagtagttc tcgatcccgg aacaatatcc cagttcccgg atcatctcca gatcataggt
      241 gacacgatct tccagacgtt tcgcttccag gtgctgcccc agtgatttca tgtactccac
      301 ctgcttcatc atgtcttcct gtatctgcca gatggcctgc agttggcgtt caggattcgt
      361 gacaaagatg ttcgccgggt agatggcaac ggcgttgtgc ttttcaaggg tctttccgtt
      421 gatcggatcg atcgaggcga tttcttcgat ctcatctccg aaaaagtaga aacggtaggc
      481 aatgtcaccg tatgcgatga agacgtccac cgtatcgccg cgaacgcgga acttcccccg
      541 gtcgaaatcg gtgtcggttc tggaatagag ggaggtgacc agtttgtgaa ggaacgcgtt
      601 ccggctgatt ttctctcctt ccctgacacg gatcgtattc gccttaaaat cacccgggtt
      661 tccgataccg taaatgcagg ataccgagct tacaaccaat acatcctgtc ggcccgacaa
      721 gagggaagtg gtggtactca gccgaagctt ctcgatctca tcattgatcg cgaggtcctt
      781 ttcgatatag gtattggtgc ttggtatgaa cgcctcgggc tggtaatagt cgtagtagga
      841 gacgaaatac tccaccgcgt tctccggaaa gaactgtttg aactcgccat acaattgggc
      901 ggcaagcgtc ttgttgtgac tcaggatgat cgtcggacgc tggacctgtt cgatgacatt
      961 ggcaacggta aaggtcttcc cggatccggt cactccgagc agtgtttggg cgtggtcgcc
     1021 gcgccgaagg ccctctacca gttgccggat cgcctccggt tgatcgccgg tagggcggaa
     1081 ttctgaaatg attttaaaag acatggaaag gacaatctgc gcttgaaagt acgaaacatc
     1141 gggaggcggt cggacaaaaa aaagaccgga tctaccggtc ttcattcttg tactgactaa
     1201 cgatcagaca taaatcggtt cgttccagat ctcccaggct ttttccgcct gtaagcgaag
     1261 catccggaat ccgttgtgaa tgaaagcgcc tgatgctttt gacttttcca aaaagcgcgt
     1321 ttggggcggg ttgtaaataa ggtcgaagca cagatgctta tgggtcagaa actcgtacgg
     1381 gatgttcgga gcgtcgttga cgttcggata catgccgagg ggtgtcgtgt tcaccacgac
     1441 ctggaacgcc ttcatggtgt tctggttgag gtcggcgtag cagtaatgga atcgggattc
     1501 ctcacgggtc acgaaaaagt gctcgatcgc caacttgcga aaaacatagc tgaccgcctt
     1561 tgcggaacca ccggttccca ggatcagggc gcgacgatgg tgcgggcgaa ggatcggtcg
     1621 gatggcgagt tcaaatcccc atgcgtccgt attgaagccg tgcaattcaa attttccgtt
     1681 ctcgaatcgc tggatccgga tcgtgttgac cgcgccgatc cgctgggaaa tatcatccag
     1741 gttatccaac atcgggatga catcctgttt gtacgggatt gtcacgttca aaccaaccag
     1801 gtcagggttg tctcgaacga tcttcttcag atcgtaaatg ttatcagagt caaaattttc
     1861 ataaacgcaa tcagtccgtc cttcacgctg aaatttttca gtaaagaact tttttgagta
     1921 cgaatgttca agtgggtgac cgaacaatcc gaaacgtttc atgatggtac taaggttagg
     1981 gttaagatta aggttctggt tcaacaagcg gttcaatagt cgtagcaggg gcgaaaactc
     2041 agcatgccgg aatcgtttaa gtcttctatc gctccgcggg tcgcagtccg ggaaaaataa
     2101 aagcggttaa tgcataaaac cttaccaatg acgtagcgtc atttctccag tgattctcga
     2161 taagccgaag ctcgcagtag ctccactaat aactccgtca caaatataca cactcttcag
     2221 cttgaatttt cctatagaaa tcttaaaaat ttgagaaatt gcaggaccgt agtagtacta
     2281 ctacattcct tcatctatgc tacctgaagt aggcgataaa gtccggtttc tcaatgatcc
     2341 nnnnnnnnnn ggaacggtac gtcggcttct gcccaacagc agggcagaag taattgattc
     2401 agatggattc acccatatta tcgagttgca tcaactcgtc aggatcgagt tcggactgga
     2461 tgaacaactg accgttccgg ctgaagcgag ttcgacttca agacccgttg agaaactagt
     2521 ggaggccacc caggctgcca ctgctaagtc ggcagatacc tggatcaggt tcatggatca
     2581 ggatcagacc gtgtatgtgg caaccgcact caagagaccg gacgctccat tggtttccga
     2641 tattgatatc cgtttgatca acaacacgca ttggaccatc ctcttcactg tagcgcgtaa
     2701 aatgccggat gtgcgatcag gggtacgctc cggtatcctg cagtcgagaa aggaggaaag
     2761 ccttggcgtc ttctccgcgg atgaactgca ccgtatcgag gggttcgagt tccagatcct
     2821 tttcttcgga gagcgcgagt atgcgttccg accgcccatc gttcggtcac tcagtatcag
     2881 tccgtcggat tttctggaac cctcttttcg cgataagcta cccgggcatc cggagactgt
     2941 cctcttgcat tccctggcag aagtaatgcc gaaaggggaa gtggatgttt cccgcctgct
     3001 ggaaaagttc agtaagtcgg aagcggaagc gaaggaacgg cagggcggag caaagccggc
     3061 acggggaagt aaattcacca tcctttcacg ggagcgtatc gtcgacttgc acatagagga
     3121 attgatcaag gatcctaccg gtatgagcaa cgcgcagatc atatcctatc agctcaacca
     3181 tttccaaaag gaacttgacc gtgctttgtt ggataaattg cacaagatca cgttcattca
     3241 cggtgtggga agtggcgtac tgcgcagttc catccgggaa gaattaaaaa aataccccgc
     3301 catccgattc caggatgcgc cgcctgaaca gttcggctat ggcgccaccg aagtcatatt
     3361 ccagtaatga acaccatgga tctggaacag gctgccacgg agaagatccg catcatgcgg
     3421 ggaattctga tttt
//
LOCUS       DAOTON010000228         3433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21712_length_3433_cov_3.605980, whole genome shotgun sequence.
ACCESSION   DAOTON010000228 DAOTON010000000
VERSION     DAOTON010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21712_length_3433_cov_3.605980"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    449..548
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1149..2489)
                     /gene="accC"
                     /locus_tag="P5292_05610"
     CDS             complement(1149..2489)
                     /gene="accC"
                     /locus_tag="P5292_05610"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRS38639.1"
                     /translation="MFKKILIANRGEIALRIIRTCKEMGINTVAVYSTADKDSLHVRF
                     ADEAVCIGPPPSRDSYLNIPRIMAAAEITNADAIHPGYGFLSENSKFSAICAEHGVKF
                     IGATPEQIDAMGDKSNAKDTMKRAGVPTIPGSDGLLTDVKTGLKIAKQIGYPVILKAT
                     AGGGGRGMRIVWKPEDLEPAWSSAKQEAGAAFGNDGMYLEKYIEEPRHIEIQIAGDQY
                     GKACHLSERDCSIQRRHQKLLEETPSPFMTAELREKMGEAAIKAAQAVKYEGVGTVEF
                     LVDKHRNFYFMEMNTRIQVEHPVTEEVINYDLIKEQIKIAAGIPITGKNYFPAMHAIE
                     CRINAEDPYNGFRPSPGKITTLHVPGGHGVRVDSHIYAGYVIPSNYDSMIAKLITIAQ
                     TREEAINTMERALSEFVVEGVKTTIPFHQQLMRNEEFRKGNYTTKFLETFQLEE"
     gene            complement(2594..3076)
                     /gene="accB"
                     /locus_tag="P5292_05615"
     CDS             complement(2594..3076)
                     /gene="accB"
                     /locus_tag="P5292_05615"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRS38640.1"
                     /translation="MNLKDIESLIQFVQKSGVSEVSLEQKDFKLIIKTSHGPAVSIPV
                     QHMAPAPVPHIAAVPAAAPAPAAPKAPEAPKASDESNYVTIKSPMIGTFYRSSSPDKP
                     AFVNVGDEVKAGKVICIIEAMKLFNEIEAEVSGRIVKVLVDNASPVEYDQPLFLVEPA
                     "
BASE COUNT          675 a          862 c          981 g          815 t
ORIGIN      
        1 gcgccaaaga actccgcgct gccaaagcgt tttcgcaggt gcactatggg cggttcttgg
       61 cgatgtaggt ttttagtgaa cagtgaacag tgaatcttgc ccaccggagc tttagcgaag
      121 gtgggagtga attttgcctg ccgtagcttt agctagggca ggagcgaata gtttgtgctg
      181 catcctgtat ctaccgtgta gctagcgggg tttgggaatg cttatttact tccgagattg
      241 tagcccccga gtagcccgac atttttgaca aagggagtcc cccatctcct tcagccccgg
      301 aggagcgtaa caagcgttga atcgaagacg ctactatgcc tgcgcatcaa atcgaaagag
      361 acgcatcagc gacttgtaca ttgcgacctg gcaattgcgg gtcggataag gtttgatccg
      421 aaaacccctc ttcgtgccca ataaaattnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnngg aggggctcga catctttgac tatggaattc ccaatctcct tcagccccgg
      601 aggggcgtaa caagcgttga atcgaaggcg ctactatgcc tgcacatcaa atcgaaagag
      661 tcgcgacagt gacttgtgta gcgcagcccg gccattgcgg gtcggataag gtttgatccg
      721 aaaacccctc ttcgtgccca ataaaattgc cccggagggg ctcgacatct ttgactatgg
      781 aattcccaat ctccttcagc cccggaggag cgtaacaagc gttgaatcga aggcgctact
      841 atgcctgcgc atcaaatcaa agaggcgcga cagtgacttg tgtagcgcag cccggcaatt
      901 gcgggtcgga taaggtttga tccgaaaacc cctcttcata cccaatatca tagccccgga
      961 ggggcccaat attaatgaca aacggattat tcaatttctt tcagccccgg aggggcgtaa
     1021 caacattcga ttcagcgctg tttcggttgt ttgcgatcca tgcggtcggt gcccctatcg
     1081 gcagttgctg cagaataaaa aaacccggaa gaatgcttcc gggtttttgt ttgctctgag
     1141 gatacgggtc attcctccag ctggaacgtc tcgaggaact tcgtggtgta gttgccttta
     1201 cggaactctt cattccgcat cagttgctgg tggaacggaa tggtcgtctt cacgccttcg
     1261 actacgaact cgctcagggc gcgttccatg gtgttgatcg cctcttcgcg cgtctgcgcg
     1321 atggtgatca gcttggcgat catcgaatcg taattcgacg ggatcacata accggcatag
     1381 atgtgtgaat cgacacggac gccgtgtccg ccgggaacgt gcagggttgt gatcttgccg
     1441 ggcgacggac ggaacccgtt gtaaggatct tcggcgttga tgcggcactc gatcgcatgc
     1501 atggctggga agtagttctt gccggtgatc ggaatgcccg cggcgatctt gatctgttcc
     1561 ttgatgaggt cgtagttgat cacctcttcg gtcacgggat gctccacctg gatacgggtg
     1621 ttcatctcca tgaagtagaa gttgcggtgc ttgtcgacca gaaactcgac tgtgcctacg
     1681 ccctcgtact tcaccgcctg tgcggccttg atggcggctt cgcccatctt ctcgcgcaat
     1741 tcggcggtca tgaacgggga aggagtttct tcgagcagtt tctggtgccg gcgttggatc
     1801 gagcagtcgc gttcgctcag gtgacaagcc ttaccatact ggtcgccggc gatctggatc
     1861 tcgatgtgac gtggttcttc gatatacttt tccaggtaca tgccgtcatt gccaaaggca
     1921 gcgccggctt cctgctttgc cgagctccag gccggttcca gatcttccgg tttccagacg
     1981 atacgcatgc cgcgtccgcc gccacctgcc gtggctttga ggatgaccgg gtacccgatc
     2041 tgtttggcga tcttgagtcc ggtcttcacg tcggtgagca gaccgtcgga accgggaatg
     2101 gtcggaacgc cggcacgctt catcgtatcc ttcgcgttcg acttgtcgcc catcgcgtcg
     2161 atctgttccg gtgtggcgcc gatgaacttc acgccatgct cggcgcagat cgcgctgaac
     2221 ttgctgttct cggagaggaa gccatagccg ggatgaatgg cgtccgcgtt ggtgatctcc
     2281 gccgctgcca tgatccgcgg gatgttcagg taggagtcac ggctgggagg aggtccgatg
     2341 cagaccgctt cgtcggcgaa gcgaacatgc aggctgtcct tgtcggccgt gctatacacg
     2401 gcgacggtgt tgatgcccat ctctttgcag gtgcggatga tgcggagggc gatctcgccg
     2461 cggttggcga tcaggatctt cttgaacatg agtgatgaat tccgtcggtt cgggcaaagc
     2521 ggcgatcagg ggcctgtgag gagggtcctg cggctgctgt tacccttgaa aggggtggta
     2581 caggttgatt ggatcaggcc ggctcgacca ggaacaaagg ctgatcgtat tcgaccggcg
     2641 aagcgttgtc gaccagcacc ttcacgatgc ggcccgacac ttcagcctcg atctcgttga
     2701 acaacttcat ggcctcgatg atgcagatga ccttgccggc cttgacctcg tcgcctacgt
     2761 tcacgaacgc aggcttatcg ggtgaggagg aacggtagaa ggtgccgatc atcggcgact
     2821 tgatcgttac gtaatttgat tcgtcggagg ccttgggtgc ttccggcgct ttgggcgctg
     2881 ccggagcagg tgcggcggcg ggaactgccg cgatgtgcgg aaccggtgcc ggcgccatgt
     2941 gctgtaccgg aatggaaaca gccggaccgt gagatgtttt gataatgagc ttgaagtctt
     3001 tctgctcaag gctcacttcg ctgaccccgc ttttctgtac gaactggatc aggctctcga
     3061 tatctttcag gttcatattc agttcctggc ttttgggtga tggtttggcc gaagcggccg
     3121 tgtttggctt ctttttcatt gaaaacgcgg ttttccctac ccccataggg tgcgtgtgga
     3181 aaggggtggg agaaccaaaa gtagaaaaat tctggaataa gggaaggggg ggcggtaggc
     3241 ggtagacggt tagcagtagg caggcctccc cactgctcac tcacactgtt tctcaaactc
     3301 acactactct tttaagtgta agactaagaa acagtgcggg tgagcagtgt ttgactgctc
     3361 actcacactg tttctcaaac tcacacttct ctttctaagt ggttaaaaag tgtaagacta
     3421 agaaacagtg cgg
//
LOCUS       DAOTON010000229         3426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21785_length_3426_cov_2.868288, whole genome shotgun sequence.
ACCESSION   DAOTON010000229 DAOTON010000000
VERSION     DAOTON010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21785_length_3426_cov_2.868288"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="P5292_05620"
     CDS             <1..1285
                     /locus_tag="P5292_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRS38641.1"
                     /translation="AWVFFSNMFSTGLAFVRSVVIARGLGAGIFGTFTMVTAFVGLIQ
                     EFLNLNIGTALIRQGAIFHAEQRNDKLVALVKVSLWLSAVMAALSVVVVAGLAFFSYS
                     TFFELPDLHWFIVAYAVAASVTYLNSISRGLLRLYYKFRATSVIQMIMDVVETISITI
                     AVLVYPKDLRIFFFAVIVARFLNGFIINALAFWELRRELGPNWNLSVRLTRQELAEFR
                     KFVLGNSIGNSLKTMINQGDVLLLGALSSAVQVGFYSVSKKLAYSVLILTDPLVSSIY
                     PQLSKLLAERKFAETRRMLFRITSITVLPAILFLTAMAFLNRWVIVTLYGAEYAPAAA
                     SFFYFLIASVVGVMTFWSIPLIQSLGLVGLRIKVYVVTILGGSLLAWWLIGQWQATGM
                     AIALLSTNLFSLAYFLWAADRKIRSQESESASLQL"
     gene            1282..2025
                     /locus_tag="P5292_05625"
     CDS             1282..2025
                     /locus_tag="P5292_05625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS38642.1"
                     /translation="MMIQYNRSTFLHHPIALARFAFGDKQPLHEVMLLHSAKKLLGNR
                     FDEKIARQALQEIRSWEFVPMNQLPDVQLPGNRPTYLFGKFLYFIVRCAQPEVMVETG
                     VAHGVSSWTILNALHRNGKGKLYSIDLPNQDLRSYNPENIRQGSGWVVPDLLRQRWEL
                     RLGPSQELLPKLIAELGSIDIFFHDSDHSYENMLFEFRAVYQAVKKNGLILSDDVHKN
                     TSFTEFVAEKGILGIQFATKGGAAVKNKD"
     gene            2057..3160
                     /locus_tag="P5292_05630"
     CDS             2057..3160
                     /locus_tag="P5292_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRS38643.1"
                     /translation="MLPIYKPYLPASSLRYAHDALDSTWISSQGVYIDKAKEQLKELI
                     KSRYVVLVNNGTCATHMLALGLKFKYPHIRKIIVPNNVYVAAWNSFLYSGGFDFEIVD
                     ADLSTWNMDHGAIREISDDTALLVVHNLGNIINVPALRKRFPKAVIVEDACEGLFGSY
                     DGIPAGTGSLMGSISFFGNKTITSGEGGAVFTQDEELFEYLNSVRGQGQSSVRYVHDK
                     LGYNYRMTNVQAAILCGQLELVADIRNRKREVFDLYREQLREVDEIRFQQEEPGTEHA
                     QWMFAVRFEGFDLPTKNALELFLFENNIETRPMFYPIDRHVHLSRFKGAHAVANQLNH
                     QCLLLPSFPEMTRGQVLNVSRKIKDFLRTRGSR"
BASE COUNT          730 a          879 c          954 g          863 t
ORIGIN      
        1 tgcctgggtg ttcttctcga acatgttcag cacgggtctg gcattcgtcc ggtccgtggt
       61 gattgcccgt ggactgggag cagggatttt tggaaccttc acgatggtaa cggctttcgt
      121 cggactcatc caggaatttc tgaatctcaa catcggtacg gccctgattc gacagggggc
      181 gatcttccat gcagagcaac gaaacgacaa gctggtcgcg ttagtcaagg tgtcgttgtg
      241 gctgagtgcg gtgatggcgg ctttatcggt cgtcgtggta gccggactgg ccttcttcag
      301 ttattccacc ttctttgagt tacctgatct gcattggttc attgtagcct acgcggttgc
      361 tgccagcgtg acctatttga attccatcag ccggggattg ctccgacttt attacaagtt
      421 cagggcgacc tccgttatcc agatgatcat ggatgtcgtg gagaccatca gtatcaccat
      481 agccgtattg gtgtacccaa aggaccttcg catctttttc ttcgcggtaa tcgttgcccg
      541 tttcctaaac ggctttatca tcaatgcgct ggcgttttgg gaacttcggc gcgagcttgg
      601 cccgaattgg aatttgtcgg tacgactcac ccgtcaggaa ttggcggagt tcagaaaatt
      661 cgtgctcgga aattccattg gcaattcgct caaaacgatg atcaaccagg gcgatgtgct
      721 cttgttgggg gcgctttcca gtgcagtaca ggtcggtttt tattccgtct ccaagaaatt
      781 ggcatattcg gtgctgatcc tgacggaccc cctggtttcc tccatttatc cccagctctc
      841 gaaactgctt gcggaacgga aattcgctga aacaaggcga atgttgtttc gtatcacctc
      901 cattacggtg cttcctgcca ttctgttcct gacggccatg gccttcctga accggtgggt
      961 gatcgtaacc ttgtacggcg cggagtatgc tcccgccgca gcatcgttct tttattttct
     1021 catcgcctcg gtggtggggg tcatgacctt ttggtccatc cccctgatcc aaagcctggg
     1081 tctggtcggg ttacgcatca aagtctatgt cgtcacgatc ctgggcggaa gtctgcttgc
     1141 ctggtggctg atcggccaat ggcaggctac cggaatggcg atcgcgttgt tgagtaccaa
     1201 cttgttcagt ctggcttatt tcctgtgggc cgcagaccgg aaaattcgtt cacaggagtc
     1261 ggagtccgca tccttacaac tatgatgatc caatataacc gcagcacgtt tctgcaccac
     1321 ccgatcgccc tggcgcggtt cgccttcggt gataagcagc ccttgcacga ggtgatgctg
     1381 ttgcattccg caaagaaatt gctgggcaac cgtttcgatg aaaagatcgc gcgtcaggcg
     1441 ctccaggaaa ttcgttcctg ggaattcgtg ccgatgaatc aattgcccga cgtgcaactt
     1501 ccaggtaacc gaccgaccta tcttttcggc aagttcctct atttcatcgt gcgctgtgct
     1561 cagccggagg tgatggtgga gaccggtgtt gcgcacggcg tttcgtcctg gaccatcctc
     1621 aacgcgctgc accgcaatgg taaaggcaag ttgtattcca tcgacctgcc gaaccaggat
     1681 ttgcggagtt acaatcctga gaatattcgt cagggatccg gatgggtagt gcccgatctg
     1741 ctgcgacagc gatgggagtt aaggctgggt ccttcacagg agttattgcc gaaactgatc
     1801 gcggaacttg gttcgatcga tatcttcttt cacgatagcg accacagtta tgagaatatg
     1861 ctttttgaat tcagagcggt ttaccaggct gttaagaaga atggactcat tctttcggat
     1921 gatgtccaca agaatacttc gttcacggag tttgttgccg agaaaggcat cctgggaata
     1981 cagttcgcga cgaagggcgg agcggctgtg aagaataagg actaagagaa cgaaccgatt
     2041 acatttctgc tgactgatgc tgccgatcta taaaccctac ctgccggctt cctccctgcg
     2101 ctacgcgcac gatgccctcg attcgacctg gatatcttcg caaggtgtct atatcgataa
     2161 ggccaaggag caactgaagg agctgatcaa gagccggtac gtggtactgg tcaacaacgg
     2221 tacctgtgcc acgcacatgc tggcgctggg cttgaaattc aagtatccgc atatccgtaa
     2281 gatcatcgtt ccgaacaatg tctacgtagc agcgtggaac agttttttat attccggtgg
     2341 attcgatttc gagatcgtag acgcggatct gtcgacctgg aacatggacc atggcgcgat
     2401 ccgggaaatt tcggacgaca ccgccttgct ggttgttcac aacctcggaa atatcatcaa
     2461 tgtccccgcg ctgcggaaac ggtttccgaa ggcggtgatc gtggaggatg cctgcgaagg
     2521 gctgttcggc agttacgacg gaatccctgc cgggaccggc tcgttgatgg gttccatctc
     2581 gttcttcggg aataagacca tcacgagcgg cgaaggcggc gcggtcttca cacaggacga
     2641 ggaattattc gaatacctga attcggtaag agggcagggg caatcgtcgg tgcgttatgt
     2701 gcacgataag ctcgggtata attaccgaat gacgaacgtt caggcagcga ttctctgtgg
     2761 acagttggaa ctcgttgccg acatcaggaa ccggaagcgc gaggtgttcg atttgtaccg
     2821 ggagcaactc cgggaggtgg atgaaatccg cttccagcaa gaggaacccg gtacggagca
     2881 tgcccagtgg atgtttgccg tccggttcga aggcttcgat cttccgacca agaacgcgtt
     2941 ggagttgttc ctgttcgaga acaacatcga aacacggccg atgttctatc cgatcgaccg
     3001 acacgtgcac ctcagccggt tcaagggagc gcatgctgtc gccaaccagc tgaatcatca
     3061 atgcctgttg ctgcccagct tcccggagat gacccgtgga caagtgctca acgtttccag
     3121 aaagatcaag gattttctac gcacccgtgg gagccgatga tgggcgatca accaacacct
     3181 ggtgctccgc gaatcgcaat tttatgtggt agtgcaggag gggaccttcg tgcactgctg
     3241 tctgcacgcg aagccggatt ggtcggattc agtatcgttt cctgtttcgc tacgagtact
     3301 acgtccaccg cactggctgt cttaggacaa gccggcgctg atcccgccca tggtgaatcg
     3361 cttcgcttca aaagcaaccg ggaagaggct ttcggcactg ttgcaaggga ttgggagacg
     3421 actcat
//
LOCUS       DAOTON010000230         3411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21893_length_3411_cov_3.471395, whole genome shotgun sequence.
ACCESSION   DAOTON010000230 DAOTON010000000
VERSION     DAOTON010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21893_length_3411_cov_3.471395"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="P5292_05635"
     CDS             <1..1017
                     /locus_tag="P5292_05635"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HRS38644.1"
                     /translation="FPEPLARLLYERYGYAPYAGRKFESEKTLRSDMVSWYELGGTYG
                     RVVFDSEKHMLKWAVSGNLLIGTNALHLQADPIAYSVADTGTVIATDLDARLRYALDP
                     EGSTLGNLLAPRGAGLGTTIGVTYFKPFYRGGFDCNRSNDRVKKYKYRIGASLIDIGH
                     IRWFRQTTQVDINAQGNRAWTQLDSLRFSSMQAVQNSAVNALGGTISDAGFGSWLPTA
                     LSVQADYSILPTVYANLSWVNRIRFAPNQAVRGNQVTLSARYETRNFEANINSNLYEY
                     ERVHLGLGLRYRFLVIGTDRLPELLGLNNTRSFDLYFGLKFQSCTQLFKRSEPDCPGV
                     FSLR"
     gene            1096..1674
                     /gene="ruvA"
                     /locus_tag="P5292_05640"
     CDS             1096..1674
                     /gene="ruvA"
                     /locus_tag="P5292_05640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRS38645.1"
                     /translation="MYHHLQGRLIEKNPAYIVIECAGVGYFVNISVHTYSKIGDNENF
                     KILTYLQITEDQHALYGFADEEERRLFRLLITVSGVGCNTARMMLSSMNPGEIESCIL
                     QDDAPRLKAIKGIGEKTAQRIILELKSKLRKDVNAPSVSVASFKVKDEALTALLTLGF
                     PRASVEKTLDSLVRQQPAAGVEDLIRLALKNL"
     gene            1786..>3411
                     /gene="sprA"
                     /locus_tag="P5292_05645"
     CDS             1786..>3411
                     /gene="sprA"
                     /locus_tag="P5292_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRS38646.1"
                     /translation="MKNIRRYAFGIGTALSFITFVWAADRPARFADFSERTTDTEESL
                     SPPDSGDGDLHYPLQDRFSDPYNSPVDHNPLLLGDPSNIKTNVEYDTDENKYIINESI
                     GSMFYRNPSYMDFDEFVEQEFRKSTGDYFRQRASEEDKLSKKAFAPKITINSQVFDRI
                     FGGNTIDIRPQGSAELSFALNTNRNQNPALPERQRKVTTFNFNERIQMNVIANIGDKM
                     KLSTNYNTEATFDFENRMKIEYTGYEDDIIKKIEAGNVTFPINSQLIQGSQSLFGIKT
                     QLQFGRLMVTSVYSQQRGQARTIDVQGGAQTTNFDIRADQYESNRHFFLAQYFRNNYD
                     RALRDLNRLQTNIIITRIEVWVTSRTFVASQNLQNRNIVAFADLGENNPDTTQSFIPD
                     FVPGFDIAEPSDSSNGLYPTLGTANLTALRQVSNSSSILDPLGIPFNFSRNKNFETVE
                     NARLLQPNEYTLNSRLGYISLNTTLEPNQVLAVAFEYTANGMVHRVGELTANNTGTTG
                     TEALFVKLIRSTNFTPKTYTWDLMMKNVYSLGGF"
BASE COUNT          839 a          984 c          826 g          762 t
ORIGIN      
        1 tttccggagc cgctcgcccg tttgctctac gaacgttacg gctacgcgcc ctatgccggc
       61 cggaagttcg aatcggagaa aacactgcgc agcgacatgg tctcctggta tgaactggga
      121 ggtacgtatg gccgtgtcgt attcgatagt gaaaagcaca tgctgaaatg ggcggtaagc
      181 ggcaacctgt tgatcggcac caacgctttg cacctgcaag ctgatccgat agcgtattcc
      241 gttgcggata ccggtacggt catcgccacc gatctcgacg cacggcttcg ttatgcgctc
      301 gatcccgaag gttcgacgct gggcaatctc ctcgccccac gcggcgccgg acttggtaca
      361 acgatcggcg tcacttattt caagcccttt tatcgtggcg gctttgattg taaccgaagc
      421 aatgaccggg taaaaaaata caagtaccgg atcggagcat cccttatcga tatcggacac
      481 atccgctggt tccgacagac aacccaggtc gacatcaacg cgcagggtaa cagagcctgg
      541 acccaactgg attcgttgcg gttttcctcc atgcaggcgg tacaaaacag tgccgtgaac
      601 gccctggggg gtacgatttc cgatgctggt ttcggttcct ggttaccgac cgccctgagt
      661 gttcaagcgg attattccat cctgccaacc gtgtacgcca atctgagttg ggtgaaccgg
      721 atacgattcg ctcccaacca ggccgtacgc ggaaatcagg ttacgctttc agcgcggtat
      781 gaaacacgca acttcgaagc caacatcaac tcgaatcttt acgagtacga acgcgttcac
      841 ctcggcctcg gcttgcgcta tcgttttctg gttatcggga ccgatcgctt accggaactc
      901 ctgggcctca acaatacccg aagtttcgat ctgtatttcg gcttgaagtt ccaatcctgt
      961 acacagctct tcaagcgcag tgaacccgac tgtcccggtg tcttcagtct gcgctgaggt
     1021 cggaaacgaa tccgtacttt gtggcaaccg ctgtagaggg gaactcttct gcctggcgaa
     1081 cttccgaatc ctaccatgta ccaccacctg cagggccgac tgattgaaaa gaatcccgca
     1141 tatatcgtaa tcgaatgtgc cggggtcggt tatttcgtca atatctcggt tcacacctat
     1201 tcaaaaatag gtgataatga gaactttaag attcttacat accttcaaat aaccgaagac
     1261 cagcatgcgt tatacggctt cgccgatgag gaagaacggc gattgttccg gcttctgatc
     1321 accgtttcag gggtaggatg caatacggcc aggatgatgc tgtcgtccat gaatccggga
     1381 gaaatcgagt cctgtatcct gcaggatgat gcccccaggc tcaaagcgat caaagggatc
     1441 ggtgaaaaaa ccgctcaacg cattattctg gaattgaaga gtaaactgcg gaaagacgtc
     1501 aacgcaccgt ctgtttccgt tgcttccttc aaagtcaagg atgaggcgct gaccgccctt
     1561 ctgaccctgg gatttccacg tgccagcgtc gagaaaacac tcgacagtct ggttcgacaa
     1621 caacccgcgg caggtgtgga agaccttatt cggttggcct tgaaaaacct gtaatttcag
     1681 gccttcgaat agttgaaaag cgctatttta ggggctgcta accacaccac cattgcgcga
     1741 ccacaacttt tataagttgc tgtcgcccag atcattattt taaccttgaa gaatataagg
     1801 cgatacgctt tcggcattgg caccgccttg tcctttatca ccttcgtctg ggctgctgac
     1861 cggcctgccc gattcgcgga ctttagtgag agaacaactg atacggagga aagtttatct
     1921 ccccctgaca gtggtgatgg cgacctgcac tatccgcttc aggaccgctt ttccgatccg
     1981 tacaattctc cggtcgatca caatccactg ctgctcgggg atccgtcgaa catcaagacc
     2041 aatgtcgagt acgacacgga tgagaacaaa tacatcatca acgaaagcat cggttccatg
     2101 ttctatcgaa atccgtcgta catggatttc gacgagttcg tagaacagga attccgaaag
     2161 tcgaccggcg actatttccg tcagcgcgct tctgaagagg acaaactgag caagaaagcc
     2221 ttcgcgccaa agatcacgat caattcgcag gtgttcgacc gcatcttcgg cggtaatacc
     2281 atcgatatcc gcccgcaagg ttcggccgaa ctcagtttcg cgctcaacac gaaccggaac
     2341 caaaaccccg cccttcccga gcgacagcgg aaagtgacta cgttcaactt caacgaacgg
     2401 atccaaatga acgtgatcgc caacatcggc gataagatga agctttccac caactacaac
     2461 acggaagcga ccttcgactt cgagaaccgg atgaagatcg agtacaccgg ttacgaggac
     2521 gatatcatca agaagatcga agccggtaac gttactttcc cgatcaatag ccagctgatc
     2581 cagggctccc agagcctctt cggtatcaag acccagttgc agttcggacg cttgatggtg
     2641 accagcgtgt attcgcagca gcgcggtcag gcgcgtacca tcgatgttca gggcggcgcg
     2701 cagacgacca acttcgacat ccgcgccgac caatacgaat ccaaccgcca cttcttcctc
     2761 gcccagtatt tccgtaacaa ttacgatcgt gccttgcgcg acctgaatcg actgcagacc
     2821 aacattatca tcacacggat cgaagtttgg gtcacctcgc gtacgttcgt cgcctcccag
     2881 aacctgcaga accgaaacat cgtcgcgttc gccgaccttg gtgaaaacaa tccggatacg
     2941 acgcagtcgt tcattccgga ttttgtaccc ggctttgaca tcgccgaacc ttcggattcc
     3001 tcgaacggtc tgtacccgac cttaggcacc gccaatctca ccgcgctccg acaagtctcg
     3061 aattcctcct ccatcctgga cccgttgggg attccgttca acttcagcag gaacaagaac
     3121 tttgaaacgg ttgaaaacgc ccgactcctt caacccaacg aatacaccct gaattcccga
     3181 ctcggctaca tctcactcaa caccaccctt gaacccaacc aggttcttgc cgtcgcattc
     3241 gaatacacgg cgaacggtat ggtacaccgg gtgggtgaac tgaccgcaaa caatacgggt
     3301 acgacgggta cggaagcctt gttcgtcaag cttatccgca gcaccaactt caccccgaag
     3361 acctacacct gggacctgat gatgaagaac gtctattcac tcggcggctt c
//
LOCUS       DAOTON010000231         3411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21895_length_3411_cov_2.703814, whole genome shotgun sequence.
ACCESSION   DAOTON010000231 DAOTON010000000
VERSION     DAOTON010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21895_length_3411_cov_2.703814"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1713
                     /locus_tag="P5292_05650"
     CDS             154..1713
                     /locus_tag="P5292_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38647.1"
                     /translation="MKSYFLLLLTFLNVSVFAQSFGPDRTIFVDPDYEVREQFLSVAF
                     NGWIYNAFNYYSMPMTGYRVSVSKDNGNTWQTVIDFSVPGASGWYPQIEDMLVAGTDT
                     LNLRIVLALLGPGNGGPENFGIVRCYDASSGIPVGTEVQLGDFTTYAITDIALATDSK
                     YPMSTSSPYSVAIAYSVRNSASVDSLNYYLSEDGGLNFDGPYWLDSTAGSYGKVDISY
                     GTGQGGSDGRFGIVAERNGHLSVLFTIAGLPSWFTVPLNVDTLFSFTNAICGSPEICM
                     QSSLTPNSAGGFSTSIFAVADNPGNATKDMVSFFLPATPGGTWGLETVDGSLDNSTLP
                     AQAYSQNAGGFFTATFYNSVDGELPFYYRSQDAGGMWIDLSYVYPDTLRFSTPYPATC
                     ISEVSGTEAICSWKQVVDTGGFVSPLEAIKFDMMNLTSGVPASNGIGVDFTIFPNPSR
                     DGFNLQFKKELRNVSVELLDVTGKTVFSKAHFAVTSETGRLRLPAQASGVYFLRVTAG
                     TGTEVVKLIAE"
     gene            complement(1821..2117)
                     /locus_tag="P5292_05655"
     CDS             complement(1821..2117)
                     /locus_tag="P5292_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38648.1"
                     /translation="MVTEAAQAFQLFYPTEKVFRVVSSGALKRAGYKVISSCDKSRQI
                     RLRRNLFPFRHRDVIVHLRKDGDNLTRIEIRRDCERKCVKDLCHEVAEAFFRIF"
     gene            complement(2366..>3411)
                     /locus_tag="P5292_05660"
     CDS             complement(2366..>3411)
                     /locus_tag="P5292_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRS38649.1"
                     /translation="YSPRPHLEKIGGMEPQVRAFIERFVELSGKKVSIVRTSIAESVR
                     QLPDSCEAIVNLKRLNDIRSINAFLSAANRGLPLGGTYVGCVETKYLRRQRVLHKYPF
                     GINYFMILVDIIYKRVFPKLPLTRDLYRFLTGDRNKILSKTEALGRLYAAGFELEEKK
                     FIGNLLWFVVRKVKDPLFDYEPVYGPIFKMRRHGKGGKVIHVYKMRTMHAYSEYIQKY
                     VYENHNLAEGGKLNNDFRVSSLGKLFRKYWVDELPMILNLLKGDLKIVGVRPLSSHYL
                     SLYSEELREVRKRHKPGLIPPFYVDLPKTLDEIQESELRYLRAYEKQPLLTDLRYFCR
                     AAFNILVKMARSK"
BASE COUNT          770 a          913 c          905 g          823 t
ORIGIN      
        1 agtttcgagt ttataatatc gaaccacaaa taaccaatat ctaatatcta atatctagtt
       61 tccagttacc agttaccatc ctcccgcccc ctcacccacc ccgctcattt tttttattcc
      121 cgcaatctct tactttcaca aaaaaacatc accatgaaat cctatttcct ccttttgctc
      181 accttcctga atgtctccgt tttcgcgcag agttttggcc ctgaccggac cattttcgtc
      241 gatcccgact atgaggtgcg ggaacaattc ttgtctgtcg cattcaacgg ttggatttac
      301 aatgcgttca attattacag tatgccgatg accggttacc gggtgtccgt ttccaaggac
      361 aacggcaata cctggcaaac cgtcatcgac ttcagtgtac ccggtgcttc gggctggtat
      421 ccgcagatcg aggatatgct ggttgccggt accgacacgc tgaatctccg gattgtcctg
      481 gcgctattgg gcccgggaaa cggagggccg gagaacttcg gtatagtccg ttgctacgat
      541 gcctcgtccg gtatcccggt gggaacggaa gtacagctcg gcgatttcac cacctacgcc
      601 atcacggata tcgccctggc caccgacagt aagtacccga tgagtacttc ctcaccctac
      661 tcggtggcca tcgcctattc tgtccggaac agcgcttcgg tcgactcgct gaattattac
      721 ctctcggaag atggcggatt gaattttgac ggcccctact ggctcgactc cacagcagga
      781 agctatggga aggttgatat ttcctacggc accggacagg gaggcagcga cggccggttc
      841 gggatcgtag ctgagcgaaa cggccattta tcggtactct ttaccattgc cggattgcct
      901 tcctggttca cggtaccgct gaatgtcgat acgcttttct cgttcacgaa tgccatttgc
      961 ggcagtccgg agatctgcat gcagagttcc ctaacgccta acagcgcagg gggcttttcc
     1021 acttccatct tcgccgtggc cgacaatccc ggaaacgcta ctaaagacat ggtttctttt
     1081 ttccttcctg caacaccggg cggaacgtgg gggctcgaaa ccgtggatgg ctctctggac
     1141 aattccaccc tgcccgcgca agcgtacagc cagaacgcag gaggattctt caccgctacg
     1201 ttctacaact ccgttgacgg ggagcttcca ttctactacc gctcacagga tgccggggga
     1261 atgtggattg atttatccta tgtatacccc gatacgcttc gcttcagtac gccatatccg
     1321 gccacttgca tcagcgaggt ctccggaacg gaagccatct gcagttggaa acaggtggtg
     1381 gataccggtg gattcgtttc accgctggag gccatcaaat tcgatatgat gaatctgact
     1441 tccggtgttc cggcatcgaa tgggatcgga gtcgacttta cgatcttccc caatccgtcg
     1501 cgggacggat tcaatttgca attcaagaag gagctcagga atgtgagcgt tgaattgttg
     1561 gacgttacag gaaagaccgt cttcagcaaa gcgcacttcg cggtcacctc ggaaacagga
     1621 agattgcgac tacccgctca agccagtggt gtctattttt taagagtcac tgcaggtacc
     1681 ggcacggaag tggtcaagtt gatcgcagaa tgaccagcaa agatcctcgc acgcccgaat
     1741 ggtggcgcgg gcttcagtct tacagcaaag tcgggcgtga attctatcac gtcgtcacgg
     1801 cagtcattga cttagtttga tcagaatatc cggaaaaacg cttcagccac ttcgtggcag
     1861 aggtctttga cgcatttgcg ttcacaatcg cgtcggatct cgatgcgcgt gaggttgtca
     1921 ccgtcttttc gcaggtggac gatcacatcg cggtgacgaa atggaaacag gtttctgcgc
     1981 aaccgaatct gccggctttt gtcgcaggat gaaatgacct tataccctgc ccgtttcaaa
     2041 gcgccggaag acaccacccg gaatactttc tcggtgggat aaaataactg aaacgcctga
     2101 gcagcttcgg tgaccatgaa atcgcagcgc cgatcccggg ctccaaaccg gttcggcagt
     2161 tacaagttac gataaaagtg ggaatgtgca agacggttcg gcattattcc gaactaacga
     2221 gtatgatgct cccgatcggc gcaaccgggt agactccact atgtaaaagc agaactgccg
     2281 gcagtggccg gcagtggccg gcagttcaag atatttttgg tggaaatcgt aatcgatcgg
     2341 tttccgttac gacgagcagc cggtgttact tggaccgggc cattttgacg aggatgttga
     2401 acgcagcgcg acagaaataa cgcaggtcgg tcagcaacgg ttgtttttcg taagcgcgca
     2461 ggtagcgcag ttccgactcc tgaatctcgt cgagcgtctt gggcaggtcg acataaaaag
     2521 gaggaatcag tccgggcttg tgtcgcttcc ggacttcccg caattcttcc gaatacaggc
     2581 tgaggtaatg actgcttagt ggccggacgc cgacgatctt caaatcacct ttgagcagat
     2641 tgaggatcat cgggagttca tcgacccagt atttccggaa aagtttgccc agggatgaaa
     2701 cgcggaaatc attgttgagc tttcctcctt cggccaggtt gtggttttcg taaacgtact
     2761 tctggatgta ctcggaataa gcatgcatgg tccgcatctt gtagacatgg atcaccttgc
     2821 cgccctttcc atgccgacgc atcttgaaga tgggtccgta gacgggttca taatcaaaga
     2881 gcgggtcttt gaccttccgc acgacgaacc agagcaggtt gccgatgaac ttcttctctt
     2941 cgagttcgaa acccgctgcg tagagacgcc ccagggcttc ggtcttggag aggatcttgt
     3001 tccggtcacc ggtcaggaaa cgatacaggt cgcgggtcaa gggaagtttc gggaaaacgc
     3061 gcttgtagat gatgtcgacc aggatcatga agtaattgat cccgaacgga tacttatgca
     3121 acacgcgctg gcggcggagg tacttggtct ccacacaacc gacgtaggtt ccgccgagcg
     3181 gtaatccgcg gttcgcagcg ctgagaaacg cgttgatcga tcggatgtcg ttcaggcgct
     3241 tgaggttgac gatcgcttca caggagtcgg gcaactgccg tacgctttcc gcgatcgagg
     3301 tgcggacgat ggaaactttc ttccccgaca gttctacgaa acgttcgatg aaggcacgca
     3361 cctgcggctc catcccaccg atcttctcga gatgcggacg cgggctgtac c
//
LOCUS       DAOTON010000232         3401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_21969_length_3401_cov_2.496115, whole genome shotgun sequence.
ACCESSION   DAOTON010000232 DAOTON010000000
VERSION     DAOTON010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21969_length_3401_cov_2.496115"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="P5292_05665"
     CDS             <1..279
                     /locus_tag="P5292_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38650.1"
                     /translation="TMLQRGTLLSRHRILDPYYRIHHNPGILEDAVDFRLADWTREPR
                     RIGRNLARRFSRLRQSSAHMFAKILYRSFTLVWFSGYFVISRLFGINR"
     gene            complement(389..1027)
                     /locus_tag="P5292_05670"
     CDS             complement(389..1027)
                     /locus_tag="P5292_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38651.1"
                     /translation="MKKPILLFLFLSLVNLILILMISFTADSNRETIERIAPATEELR
                     EKYRSSGLSRLSPEEIKEICAKLESQISRLDRLKKNLQIAEAFFAIILVWGLTKRPWQ
                     LQPFDGLTLLLLNTLLFGLHWLFIEFGNSFEEGNNATVYLRIYTGIGLLVVQPLLFYI
                     SYRWNRLELSRKLHRQKWISVAAIVLTAASGLLALIIGIGVLCTPDLSGNIT"
     gene            1178..1366
                     /locus_tag="P5292_05675"
     CDS             1178..1366
                     /locus_tag="P5292_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38652.1"
                     /translation="MISLQANGRRYPFIATRVCTRCGRKQVRRNSRGDWPPYTDAFRF
                     EEFFSHSSNTTRQDDPIR"
     gene            1413..2279
                     /locus_tag="P5292_05680"
                     /pseudo
     CDS             1413..2279
                     /locus_tag="P5292_05680"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
     gene            2364..2783
                     /locus_tag="P5292_05685"
     CDS             2364..2783
                     /locus_tag="P5292_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38653.1"
                     /translation="MEHLKEKSWKDEAVTNRKKFLARHYTYALSRTIPGARFEVVFES
                     DLDPFHADSPQADVVVYDRVLNPHVAIEFNRHEDLLEMMLAACAALERYALDEFFICD
                     YEHNTWYRLMKNESTFQENSRSELFSLNLQELSPHHG"
     gene            complement(2830..>3401)
                     /locus_tag="P5292_05690"
     CDS             complement(2830..>3401)
                     /locus_tag="P5292_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRS38654.1"
                     /translation="LGLFITAVIVGSLGWLPLSVTFLLASVVAVGSGAISVSKAYEAI
                     DMRLLILIGGMTAFGFAMSKTGTDQWLADQLVRHLLPFGEMAIMAGFVILTVFLTQPL
                     SNAAAALVVLPVAIETAHRLSVNPMTFGVAVMLSASVSLITPFEPSCILVYGPGRYRF
                     SDFFRTGWLLTALLMAVLMLLVPILWPLH"
BASE COUNT          795 a          990 c          866 g          750 t
ORIGIN      
        1 accatgctac aaagaggaac cctgctgagc cgccatcgca tcctggaccc ctactaccgg
       61 attcaccaca acccggggat actggaagat gccgtggatt tccgtctggc cgactggacc
      121 cgggagccgc gccgcatcgg tcggaatctt gccagacgat tcagtcgctt aagacaatca
      181 tccgcccata tgtttgcaaa gatcctttac cgcagtttca cgctggtctg gttttccggc
      241 tacttcgtga tcagccggct tttcggcatc aaccgttaac agacccgccg atgctgtgca
      301 ggctgttggg gcaccgttgg cggtacaagg actatcactg cgccatattt ttatcaggaa
      361 ttaaatccgc ttcattgtaa cagcgccttc aagtaatgtt gccgcttaaa tcaggcgtac
      421 acaaaacccc gattccgata atcagtgcca gtaatccgct tgctgctgtt aaaacaatgg
      481 ccgcaaccga tatccatttt tgcctgtgga gctttcgcga tagttcaagc cggttccatc
      541 ggtaggaaat ataaaacagc agcggctgaa ccaccaggag gccgatgcct gtatatatcc
      601 gtaagtaaac ggtggcgttg tttccttctt caaagctgtt tccaaactca ataaacaacc
      661 aatgcaagcc gaagagcagg gtgttaagaa gaagtaatgt caggccgtcg aaaggctgta
      721 attgccatgg cctttttgta agtccccaca ccagtataat tgcaaaaaat gcttcggcaa
      781 tctgcaaatt ctttttcagc cggtctaaac ggctgatctg gctttcgagt ttagcgcata
      841 tctctttaat ctcctccgga cttaagcggc ttagtccgga actcctgtat ttttcgcgga
      901 gttcctccgt ggcaggggcg atccgctcga tggtttcccg gttggaatcc gccgtgaaag
      961 aaatcattag gatcagaatc aggttgacca gggagaggaa gaggaaaagg agaattggtt
     1021 ttttcaaggg ggcttttcag atctgggttc taagttttat taacggaatt ttaaaaaata
     1081 tcttgtatcc tgatcttcaa tactgatgtt attgatattt tgtatattct ggtaagttga
     1141 tgcgattttt tccatcagct ccacgagcat acggatattg atatcgttac aggccaacgg
     1201 caggcgctat ccgttcatcg ctacccgcgt atgcacccgc tgcggccgca agcaggtacg
     1261 ccggaacagc cgcggcgact ggccccccta caccgacgcc ttccggttcg aagagttctt
     1321 ttcccacagt tccaacacga cccgacagga tgacccgatc cgctgaacat cgcctcccga
     1381 ccttgctgct attcgttttc tttgcagcga tcatgcgcac cggttccgca caaacagccg
     1441 cactcgacct gcagggccat cggggttgtc gtgccctcct tccggaaaac accatcgccg
     1501 gctttatcaa ggcactcgat ctcggcgtca ccacattgga actggatgtc gtgacctccg
     1561 ccgacagcca ggtggtcgtc tcccacgaag cctggatgtc gtccgagatc tgcaccggtc
     1621 cgggcaaccg gatacccgat ccgtcgggcg aagaggcact gaaccttttt caaatgaact
     1681 atgcggaaat cagcggttgg gattgcggac gacgcggtca tccccgcttt ccgcagcaac
     1741 agccacgggc ctcggtcaaa cccctgctgc gtgacgtatt ccaggcctgt gaacagcacg
     1801 ttcgggaaac gagctgatcg ccggtcacct acaacatcga aataaaatcc gccccggaaa
     1861 cggacgggcg ttttcatccc gaaccggaga cctttgttcg actgttacgg tccgtcatcc
     1921 cggactccct gaaccccgca cgcatcaccg ttcagtcctt cgacctgcgt ccgttacggc
     1981 tccttcataa gagcggtgct ccctacgcta tttccctgct ggtcgacgaa cacgacgacc
     2041 cggaagcaaa gctcctggca ctcggcttca cccccgatgc gatcagccct tactaccgac
     2101 gcgtcgacgt cgacctgatc cgcatttccc atgcgcgcgg catgcgcgtc ctgccctgga
     2161 cggtcaacga tccggctgct gtacggcaac tggcggtctg gggagtcgac ggattgatca
     2221 ccgacgatcc cgaaatggcc gctgttgtcc tgcacgcatg cagaagatcg gtccgctgat
     2281 tccgctttaa attatcgtta agcctgcacc ggttcaatct cccggaaggg gttcctcgat
     2341 atctttccgt caatcgtttc gacatggaac acctgaaaga aaaatcctgg aaggacgaag
     2401 ccgtcaccaa ccggaaaaaa ttcctggcgc gccattacac ctatgcattg tcgcgcacga
     2461 tcccgggcgc tcgctttgaa gtggtgtttg aaagcgacct ggaccctttt catgccgaca
     2521 gtccgcaagc cgacgtagtc gtgtacgacc gcgtcctcaa cccgcacgtc gccattgaat
     2581 tcaaccgaca cgaggacctg ctcgagatga tgctggcggc ttgtgcagca ttagaacgtt
     2641 atgcgctgga tgaatttttc atctgcgact acgaacataa cacctggtac cggttgatga
     2701 aaaacgaatc gacctttcag gaaaacagtc gctcggaact tttctccctc aacctgcagg
     2761 agctttctcc gcatcacggt taaacaccgg ttgctgtaaa ggagtagatg atccgggata
     2821 atgttccggt cagtgcagcg gccacaagat cggcaccagc agcatgagta ccgccatcag
     2881 caaggccgtc agcaaccagc cggtgcggaa aaagtcggaa aaacggtaac gtcccggtcc
     2941 gtaaaccagg atacaggagg gctcgaaggg cgtgatcagc gaaacgctgg ccgacaacat
     3001 cacggcaacg ccgaaagtca tcggattcac cgaaagccga tgcgccgtct cgatcgccac
     3061 cggtagtacc acgagcgcgg cggcggcatt cgacaacggt tgcgtaagga aaacggtcag
     3121 gatgacaaag ccggccatga tcgccatttc accgaagggc aacaggtgtc gaacgagctg
     3181 atctgccaac cactgatcgg tgccggtctt gctcatggca aatccgaatg ccgtcattcc
     3241 cccgatgagg atcagcagtc gcatgtcgat tgcttcgtag gccttgctca cgctgatcgc
     3301 acccgaaccg acggcgacga ccgatgcgag caggaaggtc accgataacg gcagccagcc
     3361 cagtgatccg acgatcacgg cggtgatgaa taaccccaac g
//
LOCUS       DAOTON010000233         3380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22137_length_3380_cov_3.286917, whole genome shotgun sequence.
ACCESSION   DAOTON010000233 DAOTON010000000
VERSION     DAOTON010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22137_length_3380_cov_3.286917"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..836
                     /locus_tag="P5292_05695"
     CDS             207..836
                     /locus_tag="P5292_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HRS38655.1"
                     /translation="MKSFTLNGDLRAAQSKQELKKLRAEGKVPCVLYGGSEQVHFSAP
                     VLAFKNLVYTPNVYTVELNIAGKTYKAVMKELQFNPVTDALQHIDFMELNENKPVVME
                     VPVKVSGNAIGVRAGGALITKLRRLKVSALPKHLPDSIEVKVDNLDIGDDIRVRDLKL
                     EGVTFLDTASSVVTGVKTTRNTAAAAPAAAAPAAAAAAPAAAAPAPAKK"
     gene            complement(910..3267)
                     /locus_tag="P5292_05700"
     CDS             complement(910..3267)
                     /locus_tag="P5292_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS38656.1"
                     /translation="MKNKLLLTILLTFLHYFSFGQVLTQTVRGTVVDMISQAPLPGAT
                     VVVLGVEPIIGTAADIDGNFRLNKVPVGTRSLKISFLGYKERVLPNITVTSGKEVVLQ
                     VTLEENVVQGKEVTIVAQVEKDKPLNEFSTVSARTFSVEETQKYAAAVNDPARMSLAY
                     AGVVATDDGNNNIAIRGNSPNGLLWRMEGVEIPNPNHFSNVGTSGGGISILSAQTLTN
                     SDFMTGAFPAEYGNANSGVFDLKLRKGNNQKTERTIQAGFLGTDLAIEGPFKKGYNGS
                     YLINYRYSTLSMLGALGVPVGDAITNFQDISYNVFLPTEKAGNFSLFGFGGLSNQNFD
                     AKKDSSAWKNNYDRYSWNFRSNTGAAGLTHFLQLDSKTYLRSVFLVAGTGKGYEEERL
                     DDEYAAVSQGELVYDQHRVTLSTSLTRKINAQHSYKTGIIANELFYDFLNTDRKDEED
                     RIVTVIDQKGQATLLQGYGQWNYKPSERVTINAGLHALTLTTNKTYSIEPRASVKVDI
                     RPGQAISFGYGLHGQVQPLGVYYSRQYTSDGTYTEPNKDLGFTKSHHFVLGYDRSLNE
                     FTRVKAEVYAQFLYNVPVGTEANSTYSMLNSEGGFTDKELRNSGLGRNYGFELTVERF
                     LHKDFYFLLSSSVYDSKYKAQDGRWYDTRFNGNFAGSFTAGKEFRTGEKFKKRIVGVN
                     LKAIYTGGLRNTPIDVPASVAAGETKYQEDRPFILQAPNYFRTDVKFSLKRNREKSSV
                     TWSLDLQNATNRKNVFGDYFDPKTGTTKTAYQMTMIPVLSYKVDF"
BASE COUNT          775 a          871 c          871 g          863 t
ORIGIN      
        1 gtatcagcca gttgttcctg gtgtgagagt agcgaatagc gaatagccaa tagcgaatag
       61 cgaatagtaa acagtgaaca gtgaacagtg aatggcagtt tcacatctga atagaattct
      121 aattactaat ctctagttcc tagttactaa ttacaagtta ccaataacaa atatccaacc
      181 accaatatcc aataaccaac atcaaaatga aatcattcac acttaacgga gacttgagag
      241 cagctcagtc taagcaggaa ttgaaaaaac tgcgagcaga gggtaaagtt ccgtgtgtgt
      301 tgtatggtgg ctccgaacag gtccacttca gcgcaccggt ccttgccttc aagaacctgg
      361 tgtacacccc gaacgtgtat acggtagaac taaacattgc tggcaagacc tacaaggctg
      421 tcatgaaaga actgcagttc aatccggtga cggacgcact gcagcacatc gacttcatgg
      481 agctgaatga gaacaagccg gttgtcatgg aagtaccggt aaaggtatcg ggtaatgcaa
      541 tcggcgttcg cgccggtggt gccctgatca ccaaactgcg ccgcctgaaa gtttcggctc
      601 tgccgaagca cctgccggat tccatcgaag tgaaggtcga taacctcgat atcggcgacg
      661 atatccgcgt tcgcgacctg aagctcgagg gcgtcacttt cctcgatacg gcttcgagcg
      721 ttgtgaccgg cgtgaagacg acccggaata ccgcagccgc agctcctgca gccgctgcac
      781 ccgccgctgc cgccgccgct cctgctgccg ccgcacccgc gcctgccaag aagtaatttc
      841 ctacgattgg aaatgcgaac ggcccttctc caggaagggc cgttcttttt ttattgctgc
      901 ctgatccggt cagaaatcca ctttgtagga cagtaccggg atcatcgtca tttggtaagc
      961 ggtcttggtc gtaccggtct ttggatcgaa gtaatcgcca aacacgttct tccggttggt
     1021 cgcgttctgg agatccaggc tccaggtgac gctgctcttt tcccggttcc gcttcaggct
     1081 gaattttaca tcggtgcgga aataattcgg tgcttgtaat ataaacggac ggtcctcctg
     1141 atacttcgtc tctccggctg caacggaagc cggtacatca atgggagtgt tccgcagccc
     1201 gccggtgtag atcgctttca ggttgacgcc cacgatgcgt ttcttgaatt tttctcctgt
     1261 gcggaactcc ttacctgcag tgaagcttcc ggcgaaattc ccattgaagc gcgtatcgta
     1321 ccaacgaccg tcctgtgctt tgtacttgga gtcgtatacc gaggagctca gcaggaagta
     1381 aaagtccttg tgcaggaacc gctcgacggt gagctcgaac ccgtagttgc gacccagtcc
     1441 gctattacgc agttccttgt ccgtgaaccc gccctcgctg ttcaacatgg agtacgtact
     1501 gttcgcttcc gtgccgaccg gaacattgta gaggaattgg gcatatactt ccgcctttac
     1561 ccgggtgaat tcgttcaggg agcgatcgta acccaagacg aagtgatgtg atttcgtgaa
     1621 gccaagatcc ttgttcggtt ctgtataggt tccgtcagag gtgtattgcc gtgagtagta
     1681 aacacccagc ggctggactt gtccgtgcaa accgtaaccg aagctgatcg cctggccagg
     1741 gcggatatcc acttttaccg atgcccgtgg ttcgatcgag taggtcttgt tggtggtcag
     1801 cgtcagtgca tgcaagccgg cgttgatggt tacgcgttca ctcggcttgt aattccactg
     1861 accataaccc tgtaataatg ttgcctgacc tttctggtcg atgacggtga cgatcctgtc
     1921 ttcctcgtcc tttcggtcgg tgttcaggaa atcgtaaaac aattcattcg cgataatgcc
     1981 cgtcttgtaa ctgtgttgcg cgttgatctt ccgcgtcaac gatgtgctga gcgtgacgcg
     2041 gtgctggtca tacacgagct cgccctggct gacggctgcg tattcgtcat cgagccgttc
     2101 ttcttcatag ccttttccag ttcccgcgac caggaaaacg cttcgcagat aagttttgga
     2161 atccaattgt aagaagtggg taagtcccgc cgcgccggta ttactgcgaa agttccaact
     2221 gtagcggtcg tagttgttct tccaggcaga cgagtctttc ttggcgtcaa aattctggtt
     2281 gctcaaaccg ccgaatccaa agagggaaaa gttcccggct ttttcagtag ggaggaagac
     2341 attataagag atatcctgaa agttggtgat cgcgtcgcca accggaacac ccagggctcc
     2401 gagcatggaa agggtggagt agcggtagtt gatgagatag gagccattat accctttctt
     2461 gaaaggccct tcgatcgcca ggtcggttcc gagaaatccg gcctggatgg tgcgttcggt
     2521 cttctggttg ttccctttgc gaagcttcaa atcgaaaaca cccgaattcg cgttgccgta
     2581 ttctgcaggg aatgcgccgg tcatgaaatc ggagttggta agcgtctgcg cgctcaagat
     2641 ggagattcct cctcctgaag ttccgacatt gctgaagtgg tttggattcg gaatctccac
     2701 accctccata cgccataaca atccgttcgg ggaattgccg cggatcgcga tgttgttgtt
     2761 gccgtcgtcc gttgccacca caccggcata ggccaacgac attcgggcgg ggtcattcac
     2821 cgcggccgcg tatttttggg tctcttcgac ggagaatgtt ctggcactga cagtgctgaa
     2881 ttcgttcagc ggtttgtctt tttccacctg agcgacgatg gtgacttctt ttccctgcac
     2941 cacgttttct tcgagcgtca cctgcagaac cacttctttc cctgaagtga ccgtaatgtt
     3001 cggcaggaca cgttccttat aaccaaggaa gctgatcttc aacgaacgcg ttccaaccgg
     3061 cactttgttt aaacgaaaat tgccgtcgat atcagcggca gtaccgatga tcggctcaac
     3121 acccaacacc accaccgtcg caccgggaag cggagcttgc gagatcatgt ccacgaccgt
     3181 accacgcacc gtctgcgtca agacctgacc aaaggagaaa tagtgtaaga aggttaaaag
     3241 aatcgtcaga agaagtttgt ttttcatgat tggagggggg ggattggtaa ttggttatta
     3301 gtaacttgta actagtaact agtattcgtt ttggtatcca ctatttacta tttactattc
     3361 gctattcact attcactatt
//
LOCUS       DAOTON010000234         3378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22162_length_3378_cov_3.170629, whole genome shotgun sequence.
ACCESSION   DAOTON010000234 DAOTON010000000
VERSION     DAOTON010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22162_length_3378_cov_3.170629"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="P5292_05705"
     CDS             <1..977
                     /locus_tag="P5292_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38657.1"
                     /translation="DATCFGGDGSITVSATGGSEPYSGVGTFNVPAGNYTYTVTDFAG
                     CTASCSATVAEPAEIIVASFTPTSGSVGSSVTISGSGFTGATDVSFNGTSASFTVDND
                     GQITVTVPVGATSGPITVSVGSCSGVSAANFNINTTALLNVKAFIQGYYAGSGTMNSP
                     MFNNGVSPDPSDCDTITVCLMDEFTYASVACATVIMDVNGQATVSVTIPGNYWIKITH
                     QNALQTWSAGPISISGTTTYDFTTSFTQAFGGNMVEVETGIWAFWSGDTNQDELIEAT
                     DYSNVENDVVGFLFGYLGTDLTGDGLVEASDYSLVENNAQGFLFSVHP"
     gene            complement(1048..2427)
                     /locus_tag="P5292_05710"
     CDS             complement(1048..2427)
                     /locus_tag="P5292_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HRS38658.1"
                     /translation="MSSASEQFLADAEQRVFDPEHRRKLAFNILQYDKKVVEGKQQYA
                     DFDTVRHRANFLKWKVMENLDKYLIEFEANFIRRGGKVIWARDAEEAQAELLKILKQV
                     GARTVVKSKSMTTEEVHVNEALAGAGIESIETDLGEFIVQLREEHPYHIVTPAMHLSK
                     EDVAKTFHEKYQLPIESTPKEITAFVRKTLREKYQRAEVGITGANFLIADTGGVAVTE
                     NEGNGLMSISFPRVQIAIAGIEKIIPSLADLDIFWPLLATHGTGQNITVYNSISSGPL
                     QLDETEGPEEMYVILLDNGRTQLLAQQEQRQALYCIRCGACLNGCPIYKGVGGHAYGS
                     TYSGPIGSVITPHYKGMEEFKHLSYASSLCGKCTEVCPVRIELHKLLLLNRRDSVDQH
                     TQPRSENWVYAIWKRAMLSRTLMNLGGSGIRNFVLKNFFSKSWGSRRELPKVADRSFN
                     QQWRDRMKR"
     gene            complement(2450..>3378)
                     /locus_tag="P5292_05715"
     CDS             complement(2450..>3378)
                     /locus_tag="P5292_05715"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRS38659.1"
                     /translation="VSFEEIRRGVQAGVVVNIDNLSTLEQFGNEFADRYPCCIRLNPH
                     ILAGGNTHIQTGHIDSKFGISIHQLRHIERIVKSYRLRINGLHIHTGSEILDASVFLR
                     VAELLFEAAGQFPDLEFIDFGSGFKVAYKEGDVVTDLEELGRDMSARFREFCQAYGRP
                     LQLWFEPGKYLVSESGLLLVRANVVKQTLATVFVGVDSGQNHLIRPMFYDAYHRIVNL
                     SNPTGKPRVYTVVGYICETDTFGLDRRLNEVREGDILAICNAGAYGFAMSNNYNSRLR
                     PAEVLIYKEKAYLARKREVLDDLLRNQVEVPL"
BASE COUNT          771 a          954 c          862 g          791 t
ORIGIN      
        1 ccgatgctac ctgcttcggc ggcgatggtt cgattaccgt gagtgctacc ggcggatctg
       61 aaccgtacag cggtgtcggt acgttcaacg ttccggcagg caactatacg tacacggtta
      121 ccgactttgc aggttgtact gcatcctgct ccgcaacggt ggcagaacct gccgagatca
      181 ttgttgcttc gttcacaccg accagtggct cggttggcag tagcgtcacg atcagcggct
      241 ccggcttcac cggcgctacg gatgtttcct tcaacggaac gtcggcatcg ttcacggtcg
      301 acaacgatgg tcagatcact gttaccgttc cagttggcgc taccagtgga ccgatcacgg
      361 tctccgttgg aagttgttcc ggcgtttcgg ccgccaactt caacatcaat accaccgcct
      421 tgctgaatgt gaaagcgttt atccagggct attacgctgg cagcggtacg atgaattctc
      481 cgatgttcaa taacggtgtt tcacccgatc catcggattg tgatacgatt accgtctgcc
      541 tgatggatga gttcacctat gcttccgtag cctgtgctac cgttatcatg gacgtaaacg
      601 gtcaggcgac tgtgtcggtg accattccgg gtaattactg gatcaagatc acgcaccaga
      661 acgctcttca gacgtggagt gctggtccga taagtatcag cggaacgacc acctacgact
      721 tcacgacgtc attcacacag gcttttggcg gcaacatggt ggaagtagaa accggaatct
      781 gggcattctg gagcggcgac accaaccagg atgagttgat cgaagcgacc gactatagca
      841 atgtcgagaa cgatgtcgta ggcttcctgt tcggctacct gggaacggac ctcaccggcg
      901 acggactcgt agaggcttcc gactattcac tcgttgaaaa caacgcacaa ggtttcctct
      961 tctctgtaca tccgtaaacg aagaggtaga aaaaatgaaa agggccggct cattcgagtc
     1021 ggcccttttc attttagtac acaaaggtca tctcttcatc cggtcccgcc attgctgatt
     1081 gaatgaccgg tcggccacct tcggcaactc gcgccgcgaa ccccaggact tgctgaagaa
     1141 attcttcaaa acgaaattcc ggatacccga acctccgagg ttcataagcg tccggctgag
     1201 catagccctt ttccaaatcg catacaccca attttcactt ctgggttgcg tatgctggtc
     1261 caccgaatcc cgacgattca gcaagagtag tttgtgtaac tcaatacgga cgggacaaac
     1321 ttccgtgcat ttgccgcaga gcgaagaagc atagctgagg tgcttgaatt cttccatgcc
     1381 cttataatgc ggtgtgatca ccgagccgat cggaccgctg taggtggagc cgtatgcatg
     1441 tccgccaaca cccttgtaga tcggacaacc gttcaggcag gcgccgcacc gaatgcaata
     1501 cagggcctgc cgctgctctt gttgagcgag caattgggtg cgtccgttgt cgagcaggat
     1561 gacatacatc tcctcagggc cttcggtttc gtccagctgc agggggccgg aagaaatgct
     1621 gttatatact gtaatattct gacccgttcc atgcgtcgcg agcagcggcc agaagatatc
     1681 caggtcggcc agcgacggaa tgatcttctc gattccggcg atggcgattt gcacgcgtgg
     1741 aaaggagatc gacatcaatc cgttgccttc attctccgtt actgccacac cgcccgtatc
     1801 cgcgatcagg aaattcgctc ccgtgattcc cacttccgcc cgctgatact tctcacgaag
     1861 ggtcttgcgt acaaacgcgg tgatttcttt cggcgtgctc tcgatcggca gttggtactt
     1921 ttcatgaaac gtcttggcca cgtcttcctt cgaaagatgc atggcgggcg taacgatgtg
     1981 atacggatgc tcctcgcgta gctgaacgat gaactccccg agatcggtct cgatggattc
     2041 tatgcccgct ccggccaatg cttcgttcac gtgtacctct tccgtagtca tcgacttcga
     2101 cttgaccacg gttcgcgcgc cgacctgctt cagtatcttc aacagttccg cctgcgcttc
     2161 ttccgcatcg cgcgcccaaa tgaccttgcc accccggcgg atgaaattcg cttcaaactc
     2221 gatcaggtac ttatcgaggt tctccatcac cttccacttc aggaaattgg cgcggtgacg
     2281 gaccgtatcg aaatcagcgt actgctgctt cccttcgact actttcttgt cgtactggag
     2341 gatattgaac gcaagcttcc gccggtgttc gggatcgaaa acgcgttgct ctgcgtcggc
     2401 taaaaattgt tcagaggcgg aagacatgag aagtcacaaa attaacgaat cagagcggta
     2461 cctccacctg gttgcggagc aggtcatcca ggacttcccg cttccttgcc aggtaagctt
     2521 tctccttgta aatcagcact tcagccggtc gtaagcggga attgtaattg ttactcatgg
     2581 caaatccgta agcgccggca ttgcagatgg cgaggatatc accctcccgc acctcattca
     2641 accgtcggtc aagaccgaag gtatccgttt cacagatgta gccgacaacg gtatatacac
     2701 gcggcttacc ggtgggattg gagagattga cgatccggtg ataagcatcg taaaacatcg
     2761 gacggatcag atggttctgt ccggagtcaa ccccgacgaa aaccgtcgcc agcgtttgct
     2821 tcacgacatt ggcacgaacc agtaacagac cgctttcact caccaggtac ttgcccggct
     2881 cgaaccacaa ctgcaacggc cgaccatagg cctgacaaaa ctcccggaac cgggcactca
     2941 tgtcccgacc gagttcctcc aaatccgtta cgacgtcgcc ttctttgtac gctactttga
     3001 aaccgctgcc aaagtcgatg aactccagat cgggaaactg acccgctgct tcgaacagta
     3061 attccgcaac gcgcaggaag accgatgcat ccaggatctc ggacccggta tgaatgtgaa
     3121 gcccgttgat gcgaagccgg taacttttta cgatccgctc gatgtgtcga agctggtgga
     3181 tcgaaatgcc aaacttggaa tcgatgtgac cggtctgaat gtgcgtattt cctcccgcaa
     3241 gaatatgcgg gttcaaccga atgcaacacg gataacgatc ggcaaactcg ttgccgaatt
     3301 gttcgagcgt ggaaagattg tcgatgttca ccaccacacc agcctgaaca ccgcgacgga
     3361 tctcttcgaa tgaaacgc
//
LOCUS       DAOTON010000235         3375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22176_length_3375_cov_3.062048, whole genome shotgun sequence.
ACCESSION   DAOTON010000235 DAOTON010000000
VERSION     DAOTON010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22176_length_3375_cov_3.062048"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /gene="lpxB"
                     /locus_tag="P5292_05720"
     CDS             <1..965
                     /gene="lpxB"
                     /locus_tag="P5292_05720"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRS38660.1"
                     /translation="LAFMGFTEVLMNLRTIMRNLSWCKEDILRFQPDVLILVDYPGFN
                     LRIAEWAKRQGIRVFYYISPQIWAWKQSRVHKIKRDVERMFVILPFEQEFYARFGMEV
                     DFVGHPLLDAIAHWPGGEDEISWRKQHGLDDRPLVALLPGSRKQEIGTMLPLMLEAAK
                     TFPAFQFVIAAAPSQPLGYYRQLIGERDVRVLQGETYPVLRHACAALVTSGTATLETA
                     LFKVPEVVCYKGGRISYVIARALVKIKFISLVNLIMDREVVRELIQQDLTVDNLRREL
                     AQLLDGERRQQQLHDFELLETKLGGAGASALAARQMFRYLTVAR"
     gene            1061..3124
                     /locus_tag="P5292_05725"
     CDS             1061..3124
                     /locus_tag="P5292_05725"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRS38661.1"
                     /translation="MKKAFPFIRLLPVLLFGWWWGSLDRLLHPALLLLTGIACLILMV
                     LAYHPRGWLAYRERWIYGALASVFLFSAGYQLGASRVKALQSPFHFSHDEQATAWLLH
                     FSEAPRITASGKRLIAEVMAVEHAGGLLRAEGRVVVYAPDSLPVRLNDRFWVYDRPRK
                     LNSLPFPGTFDFTAWLADHGIHHELRISANKLVAVDPDPDPSVALRFSLYRERLLNIF
                     REAGLRNEAWAVMSALVLGYDDAVDAEVMQAFSATGTLHVLSVSGLHVGLVYAAIRML
                     LGFLNRRTFGKRLQQVLSLLFIWLYAFLTGASPAVLRSAAMLSLVLGSNLVNRPAAVW
                     NSLAASIFLLTVADPSLPADVGFQLSYAAVGGILALYPWLRERSPVRTKLGQRVWEFM
                     SVSVAAQWFTFPLGLYYFHQFPNLFLLTNAIIIPLSTLAMFAGLVLLFLGPIAGLGHL
                     LGALLQHFMDAFLALVEWMGDLPCAQTTGIWINVTQLTALSLLVLCIHRWVISGNGSL
                     LLAGLFSVMICLGVQVNVRQQQLSEADISVLPVRASVCLLKTGSKAFLLVDTVDLSPG
                     IRSRIDAHLNFRGIDPKVCQTYLLKNSISLRESGGMFLKEGDCLVIGKDVYWLTDGST
                     PIGRNGTIILRKAGRPLRVVSNSREQEIIADGSLRRLPVNRISNVFPVSERGYWSKSL
                     IDQTH"
     gene            complement(3112..>3375)
                     /locus_tag="P5292_05730"
     CDS             complement(3112..>3375)
                     /locus_tag="P5292_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS38662.1"
                     /translation="VIDYKGRKLAYCADLMPSVAHLPVPYVMAYDTRPLLSLGEKAAF
                     LEDAMNGNWTLFFEHDPVIECCNLQRTEKGIRQAEAFDLDQWV"
BASE COUNT          667 a          850 c          989 g          869 t
ORIGIN      
        1 aactggcgtt tatgggtttc actgaagtgc tcatgaatct gcgcacgatc atgcgtaacc
       61 tgtcctggtg caaggaagac attctgcgtt ttcaaccgga tgtcttgata ctggtggatt
      121 acccgggatt caatctccgg atcgccgaat gggcgaagcg acaaggcatc cgggtgtttt
      181 attacatcag tccgcaaatt tgggcctgga aacagtcgcg tgtgcacaag atcaaacggg
      241 atgtggagcg catgttcgtg atcctcccgt ttgagcagga gttctacgcg cgcttcggca
      301 tggaggtcga tttcgtcggc caccccttgc tggatgcgat tgcccattgg cccggaggag
      361 aagacgaaat cagttggagg aagcaacacg gtttggatga tcggccactc gtggcgctct
      421 tgcccgggag tcgcaaacag gagatcggta cgatgctgcc gttgatgttg gaagctgcga
      481 aaacttttcc cgcgtttcaa ttcgtgatcg ccgctgcacc ttcacagccc ctgggttatt
      541 accggcaatt gatcggcgaa cgggacgtcc gcgtgttgca aggagaaacc tatcccgttc
      601 ttcggcatgc gtgtgctgct ttggtgacct ccggcaccgc caccctcgag acggcattgt
      661 tcaaagttcc cgaggtcgtc tgttacaagg gcggacgaat ctcctacgta attgcccgag
      721 cgttggtcaa gatcaaattc atttcgttgg tgaatctgat catggaccgt gaagtggtgc
      781 gggaactcat tcagcaggac ctgaccgttg acaacctacg gagggagctg gctcagttgc
      841 ttgacggaga gcggcggcaa cagcaattgc acgatttcga attattggaa acgaaacttg
      901 gaggagccgg agcatccgct ctggctgcca ggcagatgtt tcgctatctg acggtagccc
      961 gataagctcg tcctttttcg cttacatgca gttgcttaaa agaggttgaa tcattcaacc
     1021 ccttcgctgg caggttccgg tgcggtagtt ttgttccggc gtgaaaaagg cgtttccgtt
     1081 tattcgcctc ttaccggtct tgttgttcgg atggtggtgg gggagtctcg accggttgtt
     1141 gcatccggca ttgctgctct taacaggaat cgcttgcctg atcctgatgg tgctggccta
     1201 tcatccgcgc ggatggctgg cgtatcgtga acgttggata tatggtgcct tggcatcagt
     1261 tttcttgttt tccgccggct accaattggg agcctcacgg gttaaagcgc tccagtctcc
     1321 ctttcatttt tcgcatgacg aacaagccac cgcgtggctg ctgcattttt ccgaagctcc
     1381 caggataaca gcttccggga agcgattgat cgccgaggtg atggcggtag agcatgccgg
     1441 tggcttgtta cgcgcagaag gccgcgtagt ggtgtatgct cccgacagtc ttcccgtacg
     1501 gttgaacgac cggttctggg tgtacgaccg tccgcgaaag ctgaacagcc ttccatttcc
     1561 gggaaccttc gatttcacag cctggctggc cgaccatggg attcatcacg aattgagaat
     1621 ctcggcgaac aagctggtgg ctgttgatcc ggaccccgat ccttcggttg cgctgcgatt
     1681 cagcctgtat cgtgagaggc tgctgaacat ttttcgggaa gccggcttac gcaatgaagc
     1741 ctgggcggtg atgtcggcgt tggtgctcgg atacgacgat gccgtggatg ccgaagtgat
     1801 gcaggctttt tccgctaccg ggacgttgca tgttctctcc gtctccggac tgcatgtcgg
     1861 gcttgtttat gccgcgatta ggatgctgct gggcttcctg aacaggcgca cattcgggaa
     1921 acgattgcaa caagtattgt cgctcttgtt catctggttg tacgctttcc ttaccggcgc
     1981 ttctccggct gtgctccggt ccgccgccat gttaagcctg gtgctcggaa gcaacctggt
     2041 caaccgcccg gctgcggtat ggaactcgct ggcggcttcg atctttctgt tgaccgtagc
     2101 ggatccgtca cttcccgcgg atgtcggatt tcaattgtcc tacgcagccg tgggaggcat
     2161 cttggccctc tatccctggt tgcgcgagcg aagtccggta cgaacgaagt tgggccaacg
     2221 cgtctgggag ttcatgtcag tgagcgtggc cgcacagtgg ttcacatttc cgctcgggct
     2281 gtattacttt catcagtttc cgaatttatt cctgctcacg aacgcgatca ttattccact
     2341 gagcacactt gcgatgttcg ccgggctggt gttgttgttc ctggggccta tcgccggctt
     2401 gggccacttg ttaggcgcgc tgttgcaaca cttcatggat gcatttctgg cccttgtcga
     2461 gtggatgggc gatttgccat gcgcacaaac aacgggtatt tggataaacg tgacccagtt
     2521 aacggcattg agcctgttgg tcttgtgcat acatcgctgg gtaatttcag gaaacgggag
     2581 cctgttgctg gcagggctgt tttcagttat gatctgtctg ggtgtccagg taaatgtcag
     2641 acaacaacaa ctttccgaag cggatatttc ggtattgcct gttcgagcat ctgtttgcct
     2701 gctcaaaacc gggtccaaag ccttcttgtt ggttgatacg gtcgacttat cgccgggaat
     2761 ccgaagccgt attgacgcgc atttgaattt tcgggggatc gatcccaaag tgtgtcaaac
     2821 ttacctgctc aagaacagca tttcactgcg ggaaagtggt gggatgttct tgaaggaagg
     2881 ggattgcctg gtgatcggaa aggatgtgta ctggctaacc gacggatcga caccgatcgg
     2941 acggaacgga acaatcattc tgagaaaagc gggtcggccg ctacgggtgg taagcaacag
     3001 ccgagagcag gagataatcg cggatgggtc tctccgcaga ttgccggtca atcgtatttc
     3061 caacgtcttt ccggtaagtg aacgaggata ttggtcgaaa tcgttaatcg atcaaaccca
     3121 ctgatccagg tcaaaggctt cagcctgccg gatccctttt tccgtgcgtt ggaggttgca
     3181 gcattcgatc accggatcgt gctcgaagaa gagtgtccaa ttgccgttca tggcgtcctc
     3241 gagaaaggcg gctttttctc cgagtgacag caggggccgt gtatcgtagg ccatgacgta
     3301 cggcaccggc aagtgagcga cagaaggcat gaggtcagcg cagtaggcaa gtttccttcc
     3361 tttgtagtcg attac
//
LOCUS       DAOTON010000236         3358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22319_length_3358_cov_3.337875, whole genome shotgun sequence.
ACCESSION   DAOTON010000236 DAOTON010000000
VERSION     DAOTON010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22319_length_3358_cov_3.337875"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="P5292_05735"
     CDS             <1..510
                     /locus_tag="P5292_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRS38663.1"
                     /translation="AERPADSDRSAAPMEFHSLVAKNLEGDLVGFDQFKGKTILLVNT
                     ASECGYTPQYEKLEKLYQQYAGRLVIVGFPSNDFGGQEPGDATQIRSFCSNNYGVTFP
                     MFEKVRTSGRDIAPVYSWLTDKSKNGWNTQAPTWNFCKYLIDGEGQLLAFFPSSVEPD
                     DPAILRLIK"
     gene            594..1529
                     /locus_tag="P5292_05740"
     CDS             594..1529
                     /locus_tag="P5292_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS38664.1"
                     /translation="MTPALTVENFRKTYGSFVAVDDLSFHVDQGDIYGFLGPNGAGKS
                     TTIRALLGLIRPTGGQINIFGQSLAADRAGAMRRIGSIVEKPDFYKNLSGRKNLELFA
                     WLSKVRDARHRIDRMLELVGLSQRDRDAVKGYSHGMKQRLGLAQALLHDPDLIILDEP
                     TTGLDPQGIVHLRKLLLQLQAEGKTIFLSSHILSEIELIATRMVIINKGRAIVEGTVA
                     ELLSREDLVVSFSFLSTPLSNTPPDDFPWIGRIQQRSSDHWILSLSDDEIPHVNEWLV
                     GRGMAVTAIQSRRRLEDLFVRLTMEEGAMKGGKSR"
     gene            1526..2365
                     /locus_tag="P5292_05745"
     CDS             1526..2365
                     /locus_tag="P5292_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRS38665.1"
                     /translation="MIRLIWLELLKIAARPRSYIGFAVIFIFCTLVQFALYADGMEYI
                     QFVTQNVEQSFVFEGKILNGSLVCFVILQALILQIPLLVALVTGDLISGEAAMGTLRL
                     WLTRPASRAYILFSKFFAGQLYTFLILVWLGILALGVSLLIFGQGDLIVLKTDELVII
                     PANDVLWRFFAALAMAFLALSLVGALSMLLSCYSDNSIGPIITTMAIIILFTIIGSLD
                     VPLFERIRPYLFTTHMNAWRSFFEEPIPWEQIRDSAIFVFGHIIVFLGIGLFHFNRKD
                     ILT"
     gene            2362..3054
                     /locus_tag="P5292_05750"
     CDS             2362..3054
                     /locus_tag="P5292_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38666.1"
                     /translation="MIRRLLPLVLLFGVMELSAQDARELLRDLNGKFRKVREYQADVL
                     IRTDISFVKALPVQATVFFRQPDQFRIKSKGIAMLPRQGFDQVVRTLADTASYVPVLQ
                     GKEVVEGRELQLLSVLPLSDTSDLILGKFWIDVSEKLIRRSQVTTRSNGTMTSDYFFG
                     SQRQYALPDSMVFTVDTRKFKIPKAVAADLNNTKSSKPIDPKAREKGKIYLRFSNYRV
                     NQGLPPDVFKSK"
     gene            3051..>3358
                     /locus_tag="P5292_05755"
     CDS             3051..>3358
                     /locus_tag="P5292_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HRS38667.1"
                     /translation="MSSSPRYHTIRLILGDQLNAKHSWFRRKDDGILYLMMEVRQETD
                     YVVHHIQKVLAFFHAMRSFAEALTAAGHHIRYLKLDDPDNQQDLTRNIDQIARATKAR
                     "
BASE COUNT          771 a          845 c          890 g          852 t
ORIGIN      
        1 gccgagcgac ctgccgattc ggatcgtagc gccgccccga tggaattcca ttcactagtc
       61 gccaaaaacc tggagggtga tctggttggc ttcgatcaat tcaaaggcaa gaccatcctg
      121 ctggtgaata cagcgagtga atgcggctat acaccccaat atgaaaagct ggagaagctt
      181 taccagcaat atgcgggccg cctagtcatt gtcggatttc cttcgaatga ttttggagga
      241 caagagccgg gtgatgcaac gcagatccgt tccttttgct cgaataatta tggcgttaca
      301 tttcccatgt ttgagaaagt gagaacatca ggacgtgata tcgcgccggt ttattcctgg
      361 ctgaccgata aatcgaagaa tggttggaac acgcaggcgc ccacctggaa tttctgcaaa
      421 tatctcattg atggagaagg tcagttgctt gcctttttcc catcatcggt tgagccggat
      481 gatccggcaa ttctccgtct gatcaaatga accagtaagt caggacaagc acaaaaagta
      541 gaaatcgctg agcattatgg gcgataaaac cttgctacct tcgttcggtc gaaatgacac
      601 ccgccttaac cgttgagaat ttccgaaaga cctatggctc ttttgtcgcg gtcgatgatt
      661 tgtcctttca tgttgaccag ggcgatatct acggcttctt aggaccgaat ggagcaggga
      721 agagtacaac cattcgcgca ctgcttggtc tgatccggcc taccggtggt cagataaata
      781 tcttcggaca atcgctcgcg gcggatcggg caggtgcgat gcgtcgtatc ggatcgatcg
      841 tagaaaagcc ggatttctat aagaacctga gtggaaggaa aaaccttgag ctgtttgcct
      901 ggctcagtaa ggtaagggat gctcgccatc gaatcgatcg gatgctggaa ctggtcggat
      961 tgtctcagcg tgatcgcgat gccgtgaaag gttattccca cggaatgaaa caacggctgg
     1021 gccttgcgca agctttgttg catgatccgg atctgatcat cctggacgag cccacgaccg
     1081 gacttgaccc acaagggatc gtccaccttc gcaaattgtt gttgcagttg caggcagaag
     1141 gcaaaaccat attcctttct tcccacatcc tgtccgagat cgagctgatc gccacccgaa
     1201 tggtgatcat caacaagggc agagcgatcg ttgaaggaac agtagcggag ttgttgagcc
     1261 gggaagatct ggtggtttcc tttagttttc tttcaacgcc tctttccaac actccgccgg
     1321 atgattttcc ctggatcggg cgcattcagc agcgttcatc tgatcactgg atactatcct
     1381 tatcggacga cgagatccct cacgtcaacg aatggctggt cggtcggggc atggctgtga
     1441 ccgctattca gtcccgccgg agattggagg atctgttcgt ccgattgacc atggaagaag
     1501 gggcgatgaa aggagggaag tcgcgatgat ccgcctcatt tggctcgagt tgttgaaaat
     1561 cgcggcccga cccagaagct acatcggctt cgcggtgatc tttatcttct gcacgcttgt
     1621 tcaattcgcg ttgtatgcgg acgggatgga gtacatccag ttcgttacgc agaacgtaga
     1681 gcagtcgttt gtatttgaag ggaagatcct gaacggaagc ctggtttgct ttgtgatctt
     1741 gcaggcgttg atcttgcaaa ttcccctgct tgtcgccctg gtcaccggcg acctcatctc
     1801 cggggaggct gccatgggta ccttgcggtt gtggttgact cggcctgcat cgcgggccta
     1861 catccttttt tcgaaattct ttgcaggaca gttgtatacc ttcctgatat tagtctggct
     1921 gggaatactg gcattaggag tcagcttgtt gatcttcgga cagggcgacc tgattgtctt
     1981 gaaaaccgac gaactggtga tcatcccggc caacgatgtg ctgtggcgat tctttgccgc
     2041 actggccatg gcatttctgg ccctgtcatt ggttggcgct ttgtcgatgt tattgtcctg
     2101 ttactccgac aactcaatcg gcccgatcat cacgaccatg gcgatcatca tcttgtttac
     2161 catcatcggc tccctggatg tgccgttgtt cgaacgcatc cgtccttatc tgttcacgac
     2221 acacatgaat gcctggcgta gtttcttcga ggagccgatc ccatgggagc agatacgcga
     2281 ctcggcgatc tttgtctttg gccatatcat cgtgtttctc ggtatcggac ttttccattt
     2341 caaccggaaa gacatcctca catgatccgt cgattattgc ccctagtact cctgtttggc
     2401 gtgatggagc tttccgctca ggatgcgcgc gaactcttgc gtgatctgaa cggtaagttc
     2461 cgcaaagtca gggagtatca ggccgatgtg ctgatccgga ccgatatcag tttcgtcaaa
     2521 gcattgcctg tgcaggcaac cgtattcttc cgtcagcccg accaattccg gatcaagtcg
     2581 aagggtattg ccatgttgcc ccggcaaggg ttcgatcagg tggtacgaac cctggccgat
     2641 acagccagct acgttccggt cctgcaggga aaagaggtag tcgaagggcg ggaattgcaa
     2701 ttgctcagcg tactcccttt atccgacact tccgatctga tcctgggaaa attctggatc
     2761 gatgtttcgg aaaagctgat tcgacgttct caggtgacca ctcggtccaa cggtacgatg
     2821 accagcgatt acttcttcgg gtcccaacgg cagtacgcct tacccgatag catggtgttt
     2881 accgttgaca cccggaaatt caagataccc aaagcggttg ccgcggatct gaacaacacg
     2941 aaaagttcaa aacccattga tccgaaagcg cgggaaaaag ggaagattta tctacgtttt
     3001 tccaattatc gtgtgaacca gggactgcct ccggatgttt tcaaatccaa gtgagcagtt
     3061 ctccacgata tcatacgatt cgtctgatcc tgggcgatca gctaaatgca aagcactcct
     3121 ggttcaggag aaaggacgat gggatcctgt atctgatgat ggaggtccgt caggaaacgg
     3181 attatgtcgt ccatcatatc caaaaggtat tggctttctt ccacgcgatg cgatcgtttg
     3241 cggaagccct gaccgcggcc gggcaccaca ttcgttatct gaaactggac gatccggata
     3301 accagcagga ccttacccga aacattgatc agatagcgcg ggcaacaaag gcgcgcag
//
LOCUS       DAOTON010000237         3350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22392_length_3350_cov_3.919879, whole genome shotgun sequence.
ACCESSION   DAOTON010000237 DAOTON010000000
VERSION     DAOTON010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22392_length_3350_cov_3.919879"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="P5292_05760"
     CDS             <1..308
                     /locus_tag="P5292_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38668.1"
                     /translation="FTIYFSVLVACMLAQVVLVHYVLVSRPQLRIGSDITDDLRELKN
                     KKIGLLSLLLCFTLSTITYYMIGDEELKTIGTLWFTLMYPFMRLMGYQKKEKKKNAK"
     gene            424..1995
                     /locus_tag="P5292_05765"
     CDS             424..1995
                     /locus_tag="P5292_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38669.1"
                     /translation="MKAVFLTAVLACAALSAIAQRTFERHWTFNNWSAGERVLEVPGS
                     GYYTIGWNDSLSFDSLGQPQIDYFEGLVTHFDYDGNAIKAFRYGNDDTLFNRLFGGNS
                     DDSFITGVVDSNNRLFLAGWTQSYGVQSFYDYDFWLVKLDTNFNIILNRNISIPDTAY
                     AMYNTVGISNPQNGITLCGYLKSLTNFIPYAHISAFDSSGNLLFRGMPLPNEPSELQG
                     IVSTSDGGYLCSGVKYNNYFSGDISPLVVKTDSIGREVWRLVLPYSNDYHFAGDLTAT
                     QDGNYVFTWGNVYRSPGATNKVWMYHASKVDEAGNILWTRDYTYSFNSRIRISEVPNG
                     RLMISGLFTDTLTFGDAALLILCDQDGYPLWTRTFIGEPGTSPAPMPFCMDANFSSDG
                     GFLLTGQTSCCNFTPNLGWTSSLWLLKTDSLGLITQVSSGPNNYSNDMHLGFPFPNPV
                     ADYCSIPVTLAPDIGQAQLLLFDLQGRQVACFPLQSGFQQLQLDLSPYPAGEYLLALS
                     INGFNAGARRLVIGR"
     gene            complement(2569..2841)
                     /gene="rpmA"
                     /locus_tag="P5292_05770"
     CDS             complement(2569..2841)
                     /gene="rpmA"
                     /locus_tag="P5292_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRS38670.1"
                     /translation="MAHKKGVGSSKNGRESHSKRLGIKIYGGQSAIAGNIIVRQRGTK
                     HFPGQNVGIGKDHTLFALIDGTVVFKKGQKDKSFVSVLPLSSEAQA"
     gene            complement(2877..3185)
                     /gene="rplU"
                     /locus_tag="P5292_05775"
                     /pseudo
     CDS             complement(2877..3185)
                     /gene="rplU"
                     /locus_tag="P5292_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569489.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
BASE COUNT          817 a          821 c          838 g          874 t
ORIGIN      
        1 cgttcaccat ctatttctcc gtcctggtcg cctgcatgct ggctcaggtc gtcctcgtcc
       61 actatgtttt ggtcagccgt ccgcaactgc gcatcggatc cgacatcacc gacgacctgc
      121 gcgaactgaa gaataaaaaa ataggactcc tctcgctgct gctctgtttt actctaagca
      181 cgatcacata ttacatgatc ggtgatgaag agctgaaaac aatcggtaca ctctggttca
      241 ccctgatgta ccccttcatg cgactgatgg gttatcagaa gaaggagaag aagaaaaacg
      301 cgaaataaac gaactttctc aagcttaaga acgggtatcg gaacacctca tatcctcaaa
      361 ctaatgatca actagcgtca ttaattcggg cagtcgcctg cccggacttt taatcaaaat
      421 aatatgaaag ccgttttcct aactgcagtt cttgcatgcg cagcactatc tgcaattgcc
      481 cagcgcacct ttgagcggca ctggacattt aataattggt ctgccggcga gcgggttctg
      541 gaagttccgg gctccggata ctacaccatc ggctggaacg actccttgtc cttcgatagt
      601 cttggccagc cacaaataga ttattttgag ggattggtca cccatttcga ttatgacggc
      661 aatgccatca aagcgtttcg gtacggtaat gacgatactt tattcaaccg gttgtttggc
      721 ggaaattccg atgacagttt tattaccgga gtggtagatt ccaataatag attatttctt
      781 gccggatgga ctcagagtta cggtgtacaa agtttttacg attacgattt ttggctagtc
      841 aagctggaca cgaatttcaa catcatcctc aatcgaaaca tatccatccc ggatacggca
      901 tatgctatgt ataacaccgt tgggatatct aacccgcaaa atggcatcac actttgcgga
      961 tacctgaaat ccctgacaaa ttttattccc tatgcgcata tcagcgcatt cgactcttcc
     1021 ggaaacctat tgttcagagg catgcccttg cccaatgaac cgagtgaact tcaaggcatt
     1081 gtatctactt cggatggtgg ctatctttgc tctggagtaa aatataataa ttatttcagc
     1141 ggggacattt ctccgttagt tgttaaaacg gattctattg gtcgcgaagt ttggagactc
     1201 gttcttcctt actcaaatga ttaccacttt gcaggtgacc tgacagctac acaagatggg
     1261 aattatgtat tcacctgggg caatgtctat cgcagtccgg gtgccacgaa taaagtctgg
     1321 atgtaccatg ccagcaaggt ggatgaagcg ggtaacatcc tttggacccg cgactacacg
     1381 tattccttta acagccgtat caggatctct gaggtaccca atggaagact catgatatcc
     1441 ggcctcttta cagacacctt gactttcgga gacgctgccc tgttgattct ctgcgaccag
     1501 gacggatacc cgctctggac ccggaccttt atcggggagc cgggaacttc accggcgcct
     1561 atgcccttct gcatggatgc caacttctcc tccgatggcg ggttcctgct gaccggtcag
     1621 acttcctgct gtaactttac tcctaatctc ggctggacca gcagcctctg gctgctgaaa
     1681 accgatagtc tcggcctcat tacccaggtc agtagcggcc cgaacaatta ttcaaacgat
     1741 atgcacctgg gcttcccgtt tcccaacccg gtagctgatt actgctccat acccgttacg
     1801 ctggcgccag acattgggca ggcacaactg ctcctgtttg acctgcaagg caggcaagtc
     1861 gcctgtttcc cattgcaatc cggctttcaa caactacaac tggacctctc accctacccg
     1921 gcaggcgaat acttgctggc attgtccatc aatggattca acgccggcgc aaggagattg
     1981 gttattggaa gataatgggg gaagtgggtg ttagtgagtg gttgttgggt gactggtaga
     2041 gagtgaacag tgaacagtaa acagtaaata gtaaatagtg aatagtgaat agtgtaccct
     2101 gcctacttta gctttagcgt ggcaggagta aatagtttgc cgttgtctgt agcataccca
     2161 ggcatgcttg ggcatgcgga ttaacgtgac gccagtcgat catataacga tgaccccata
     2221 ggggtcacgg tatcctagag gttgcataaa gggttcatcg ggtatgcggc ttggaattac
     2281 ggcatgcagg tcccccttga ggcgaagccg gagcgttgca ggctagcccc gggtgagtga
     2341 ggcgttccct gcgttgaaca atgcaggggt gttgatgcgc cgagcgaaca cgggaccggt
     2401 ggtgagaagg aagaaggcgg tgcgctgctg attattccaa atgaaggata gttattggac
     2461 aggaactcat ccaagacggc ctgtcagaaa aagtccaaaa cgaccatacg gtagaaacaa
     2521 aaaaaccccg ctactgcggg gttcttcatg agtgtcttcg gttcaggatc aagcctgggc
     2581 ttcgctgctc agcgggagaa cagaaacaaa gctcttgtcc ttctggccct tcttgaaaac
     2641 aacggtgccg tcgatcaacg cgaacagggt gtggtccttg ccgatgccga cgttctggcc
     2701 cgggaagtgt ttggtaccac gctgacgcac gatgatgttg ccggcaatgg ccgactgacc
     2761 accgtagatc ttgatgccaa gtcgtttact gtgtgactcg cgaccgttct tggaactacc
     2821 tacgcctttt ttatgtgcca tggtatgagg atgctttagt tgttaggatt gaatcaggcc
     2881 ttgatgcctt cgatttgcag gacggtcagg tcctgacggt gaccactctg cttctggtaa
     2941 cctttccggc gcttcttttt gaagatgatg actttgtcgc cacgcttgtg cgtaacgatc
     3001 ttggccgaaa cgctggctcc cttcagaacc ggagcgccta ctttcacctt accgttgtcg
     3061 tcgacgagaa gaacgtcgct gaactccagc tttttgcctt cttcgccttc catgcggttg
     3121 acgacgactt tctggtcctt ggcaaccttg agctgctggc cggcaatgtt gacgatagcg
     3181 tacatcgtat tgataattaa ctgtttacaa gaatttccct gaaatgcagg gatgcaaatg
     3241 tacggaatgg ttgagaaatg ggcaaaaaaa ttagagtaga aaatagggac tggtaactag
     3301 tgtcatgtca agcatggact gacggataaa ggcgctttag ttttatgcgg
//
LOCUS       DAOTON010000238         3347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22420_length_3347_cov_2.929526, whole genome shotgun sequence.
ACCESSION   DAOTON010000238 DAOTON010000000
VERSION     DAOTON010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22420_length_3347_cov_2.929526"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="P5292_05780"
     CDS             <1..1238
                     /locus_tag="P5292_05780"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRS38671.1"
                     /translation="EFQGRRIPMFLQPGCDIEVAPDSAAESGLSFSGKGAADAGFICR
                     FLIHYAAAFNDSLQSLAIMNTPIDAYEAKIFSMRKEQLDYCKQDPAWSALSAPARSYL
                     ETQIRYRYWALLLSYPIVIANSSREIQTVNPLPESMLDGLEKVKINEPSALVNEQYRL
                     FVRHYIYYQTSKTNGFRKFPDVSSSSDRKIQVARDRLEQPVFRIFLARFLADECGFLS
                     PFMYNKLTKELRSCDEGGSYVSAVRKICEEKRDQVYQKLAEVSPADAQAAKTGEAGIS
                     FKDLEGKQVKLDDFKGKVVYIDFWASWCGPCRAMMPFSKQLHDSLDPKLKKKVVFLYI
                     SIDSKQESWKKAVQDMGIQGENVISPGDWNSEVCTFFQVYSIPRYMLMNAKGEIVDFN
                     AKRPNDPALLQDLRRLAEE"
     gene            complement(1241..2698)
                     /locus_tag="P5292_05785"
     CDS             complement(1241..2698)
                     /locus_tag="P5292_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRS38672.1"
                     /translation="MQEQQRPRFIRLIIGPLMGCLAYWLLSTNGLDHAKAATVLVTIW
                     MGIWWITEVIDLGVTSFLPFLLFPLLGVLDANTVAAQYMEQTIFLFVGGFFLAYAMER
                     WNLHQRLAFSIILKAGSTPSRVLLGIMITTFTVSMWISNTATTVMLLAAVLAIVRHEH
                     LFPKSGHAKIATGYLLALAYTASIGGMATLVGTPPNMILAGFMDKIEPGAVNFNRWFV
                     FGFPFALTFGTIAFFFIRYRFIPKSDDLRFDRNYIREKLEALGPLQTEEKRVIAIFLF
                     AVLGWFTRIDLDFGAFRIPGWSNLLPEGKMIKDSTIAIFAALLLFLIPAKKKDAGALL
                     NWSDVTRLPFRIILLFGSGFALAEAFERTGLAQWLAGHLQVLHGMPAWIMVLGVAVMA
                     TALSEFASNVASVQLMLPILAPLAVSLEVDPLVLMFPATIAASFGYMMPVATAANTIV
                     YGTGYIPVRQMMRTGLFLNIIGILLLTLFSQLIRF"
     gene            2826..>3347
                     /locus_tag="P5292_05790"
     CDS             2826..>3347
                     /locus_tag="P5292_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013447180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HRS38673.1"
                     /translation="MQKERNHPAGLPYLFFTEMWERFGYYLIIGIFFLYMTDTERGGL
                     AFDRKDASDIFGTFIALVYLTPFIGGLLADRILGLRVAITIGGILMGLGYIGLSLPGP
                     TAFYTSLGLIILGNGFFKPNISTLLGNLYNEPKFKPRKDAGYNIFYMGINIGAFICNF
                     FAAYLRNAFGWGEA"
BASE COUNT          751 a          938 c          982 g          676 t
ORIGIN      
        1 tggagtttca ggggcgacgg attccgatgt tcctgcaacc cggatgcgat atcgaagtcg
       61 cgccggatag cgccgctgaa agcggattgt ccttttccgg aaaaggcgcg gcggacgccg
      121 gcttcatctg ccggtttctg atccattatg ccgctgcgtt caacgacagc cttcagtcgc
      181 ttgcgatcat gaacacaccc atagacgcgt acgaggcgaa gatcttttcg atgcgcaagg
      241 aacagctcga ttactgcaag caggacccgg catggtcggc actttccgct ccggcccgga
      301 gctatctcga aacgcagatc aggtatcggt attgggcgtt gttgttgtcg taccccatcg
      361 tgatcgcgaa cagcagccgg gaaatacaaa ccgttaaccc cctgcccgag tcgatgctgg
      421 acggcctcga gaaagtgaag atcaacgagc catccgcact ggtgaacgaa cagtaccgct
      481 tgttcgttcg gcattatatc tattaccaga catccaagac gaatggcttc cggaagtttc
      541 cggatgtttc atcctcttcc gaccggaaga tccaggttgc gcgagaccga ctggagcagc
      601 cggtgttcag gatattcctt gcccgttttc tcgcggatga atgcggattc ctgtcgcctt
      661 ttatgtacaa caaactcacg aaggagttgc ggtcctgcga cgagggaggt tcctatgttt
      721 cagcggtccg gaagatttgc gaagagaaaa gggatcaagt gtatcagaaa ctcgcggagg
      781 tttccccggc cgatgcccag gcagccaaga ccggggaggc cggaatcagt ttcaaggatc
      841 tcgaaggaaa gcaagtcaaa ctggacgact tcaaaggcaa agtcgtgtac atcgactttt
      901 gggcgagctg gtgtggccct tgccgggcga tgatgccatt ctcaaagcaa ctgcacgaca
      961 gccttgatcc gaaattgaag aagaaggtcg tcttccttta catctccatc gactccaagc
     1021 aggagtcgtg gaagaaagcc gtccaagaca tggggataca gggagagaat gtcatttcgc
     1081 cgggagactg gaattcggaa gtatgtacct ttttccaggt ctacagtatt ccgcgttata
     1141 tgttgatgaa tgccaaaggt gagatcgtcg atttcaacgc gaagcgaccc aacgatccgg
     1201 cgctcctcca ggacctccgg aggctcgccg aagaatgatt ttaaaatcgg atgagctgcg
     1261 agaacagtgt cagcagcaat atgccgatga tgttcaggaa aagtcccgtg cgcatcatct
     1321 gccgtacggg gatgtagccg gtgccataca cgatcgtatt cgcggccgtt gcaacaggca
     1381 tcatgtagcc gaaggaagcc gcgatcgtcg ccgggaacat caggaccagc ggatccactt
     1441 ccagggagac ggccaggggc gccaggatcg gtaacatcaa ttgcacgctg gccacgttgg
     1501 aagcgaactc gctcagcgcg gtcgccatca cggctacgcc cagcaccatg atccaggccg
     1561 gcatgccgtg caggacttgc aagtgccccg ccagccactg cgccagtccg gttcgttcga
     1621 atgcttccgc taaggcgaag ccgcttccga agagcaggat gatgcggaac ggcagtcgtg
     1681 tcacgtcgga ccagttcagc agagcgccgg catctttttt cttcgccggg atcaggaaca
     1741 acaggagggc cgcgaagatc gcgatggtgg agtccttgat catctttcct tccggcaaca
     1801 ggttcgacca accgggaatc cgaaaggcgc cgaagtcgag gtcgatgcgc gtgaaccagc
     1861 ccagcacggc gaacaggaag atggcgatga cgcgtttctc ttccgtctgc aacggcccca
     1921 gggcttccag tttttcgcgg atgtagttgc ggtcgaagcg caggtcgtcg gacttcggaa
     1981 tgaagcggta ccggatgaag aagaacgcga tcgtgccaaa ggtcaacgcg aacggaaagc
     2041 cgaagacgaa ccagcggttg aagttcaccg cgcccggttc gatcttgtcc atgaaaccgg
     2101 ccaggatcat gttcggcggc gttccgacga gggtggccat gccgccgatg ctggcggtat
     2161 aagccaatgc gaggaggtag cccgtcgcga tcttcgcgtg ccccgatttc ggaaagaggt
     2221 gttcgtgccg cacgatcgcg aggaccgcgg ccaggagcat gacggtcgta gccgtattcg
     2281 agatccacat cgacacagtg aaggtggtga tcatgatccc cagcaatacg cgggagggcg
     2341 tggacccggc tttgagaatg atggagaacg cgagccgctg gtgcaggttc cagcgctcca
     2401 tcgcgtaagc cagaaagaat ccgccaacga aaagaaagat cgtctgctcc atgtactgcg
     2461 ccgcgaccgt gttcgcgtcg agcacgccga ggagcgggaa gaggaggaag gggagaaagg
     2521 aggtgacacc caggtcgatc acttcggtga tccaccagat ccccatccag atcgtcacga
     2581 gcaccgtcgc cgctttcgcg tgatcgagcc cgttcgtact cagcagccag taggccaggc
     2641 agcccatcag cggaccgatg atcagccgga tgaaacgggg gcgttgctgt tcctgcacgt
     2701 cagcgaatgt aggaggaggc ggtcaacttc gctcgacgct gctcgccgag ttttgcaccc
     2761 gacccctgtt gtacgcctga tcattcctgc ttattttgcc gaaaggccag gaggccagga
     2821 acattatgca gaaggaacgc aaccaccccg ccggcttgcc ctatctcttc tttacggaga
     2881 tgtgggaacg cttcggctat tacctgatca tcgggatctt tttcctgtac atgaccgata
     2941 cggaacgggg cggactggcc tttgaccgaa aggatgcatc ggacatcttc ggcaccttca
     3001 tcgccctggt gtatctgaca ccgttcatcg gcggcttgct ggcggaccgg atcctcggcc
     3061 tgcgtgtggc aatcaccatc gggggcatcc tgatggggtt gggttacatc ggcctctccc
     3121 tgccgggtcc cactgccttc tacacctcgc tcggactgat cattctgggt aacggctttt
     3181 tcaagcccaa catctccacc ttgctgggga acctctacaa cgaaccgaaa ttcaaaccga
     3241 gaaaggacgc cggctacaac atcttctaca tgggcatcaa catcggtgcc ttcatctgca
     3301 atttcttcgc ggcctatctc cgcaacgctt tcggctgggg cgaagcc
//
LOCUS       DAOTON010000239         3344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22443_length_3344_cov_3.391608, whole genome shotgun sequence.
ACCESSION   DAOTON010000239 DAOTON010000000
VERSION     DAOTON010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22443_length_3344_cov_3.391608"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /locus_tag="P5292_05795"
     CDS             complement(<1..1833)
                     /locus_tag="P5292_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRS38674.1"
                     /translation="MNLNQLILLVCALLQSVHVQAQAQAIYPATPAKERLEGIARRTR
                     LTDESLFKNLPLRNIGPTIMSGRAVDLEVNPEDPNEFYVAYASGGLWHTSNNGQSFEP
                     LFDQEDVMTIGDFAVDWKTRHIWLGTGEVNSSRSSYSGVGMFFSADSGKTWQQRGLPE
                     SHHIGKVLIHPNDPKTVWVAALGHLYSPNAERGVYKTTDGGTNWQRVLSGDDTTGAVD
                     LVMDPFNPSILYATLWHRDRRAWNFVESGATSAIYKSVDGGEHWNRISGGESGFPSGP
                     GVGRIGLAISQQHPNVLYAVLDNQERSKEEEPRDTAKIEARDLREISKERFLALNDTK
                     LEKFLRNNGFPEKYTAAVVKELVKSDSIKTSAITDYLNDANNSLFDTPIIGLEIYRSE
                     DAGKTWKKTHTTRLKNVVYTYGYYFGRIAVSPIDDNRIAVCGLPLIFSTDGGKSFKPI
                     DGDNVHGDHHAVWMSAKRPGHMIICNDGGLNITYDEGKHWFKANTPPVGQFYHVTVDM
                     ATPYNVYGGLQDNGVWTGSSSTTPSTEWHASGSYPYKFLMGGDGMQTQVDYRDNATLY
                     TGFQFGYYYKLNKNQPDGAQSIQPQNDLGEPNFRFNWQTPICLSR"
     gene            1923..2447
                     /locus_tag="P5292_05800"
     CDS             1923..2447
                     /locus_tag="P5292_05800"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRS38675.1"
                     /translation="MNNAAFRYGIWISIVLLFTFLGTSLLWVEGSNQHLSVWLGYIGL
                     FVSMILLVLGILHKRNKEQGGKITFGQAFRTGATITLAAGILFFFMNWAFFEIRGDAW
                     LKKYYAHQVELIRSGAPSPEAAASRLQQFEEEWKQSGERYKNSAFQAGVVFFTLVPAG
                     FVVSLVGAWLLKRT"
     gene            2784..>3344
                     /locus_tag="P5292_05805"
     CDS             2784..>3344
                     /locus_tag="P5292_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38676.1"
                     /translation="MTHTPLHAHAGTSLNRIVRLTRSVSGALFFGFFLLISVSSALGQ
                     TAGGPIDPSIKELLNTYEHKIYFTENRGQFKERVLYRADFPYGQAAATAEGMYISTYD
                     PASVQARMDDGELLEREIQNGQRIRPLNGRLKGHTWLMNFVGRSAGMTTSGAERHGDF
                     YNYFTGNTSRHATGVANYQEVWYNNVY"
BASE COUNT          764 a          891 c          856 g          833 t
ORIGIN      
        1 gcgggacagg cagatcggcg tttgccagtt gaaacggaag ttgggttctc ccaggtcgtt
       61 ctgcggctgg atcgactgtg cgccatccgg ttgattcttg ttcagcttgt agtaatatcc
      121 aaattgaaaa ccggtgtaga gggtggcatt gtcgcggtag tcgacctgcg tttgcatccc
      181 gtcgccgccc ataagaaact tgtacggata gcttccgctg gcgtgccatt cggtggaagg
      241 cgttgtcgat gaagagcccg tccaaacacc attatcctgt aacccaccgt acacattata
      301 cggcgttgcc atgtccaccg tgacatgata gaactgtccg accggcggcg tattggcctt
      361 gaaccagtgt ttgccttcgt cgtaggtgat gttcaatcct ccgtcgttgc aaatgatcat
      421 gtgtccgggg cgtttggcgc tcatccagac cgcgtgatgg tcgccgtgca cattgtcacc
      481 gtcgatcggc ttgaacgact tgccgccgtc cgtcgagaag atcaacggca ggccgcatac
      541 cgcaatgcgg ttatcatcga tcggagaaac ggctatccga ccgaagtagt aaccataggt
      601 gtaaacgaca ttcttcaggc gcgtggtatg cgtcttcttc cacgtcttgc ctgcatcttc
      661 acttcggtag atctcgaggc cgatgatcgg tgtatcgaag agcgagttgt tggcgtcgtt
      721 caggtaatcg gtgatcgccg aggtcttgat cgaatccgac ttcaccagtt ctttgactac
      781 cgcagcggta tacttttccg ggaatccgtt gttgcgcagg aatttttcca gcttcgtatc
      841 gttgagcgcg agaaaccgtt ctttggaaat ctcccggagg tcccgggctt cgatcttcgc
      901 ggtatcgcgc ggttcttctt ccttgctgcg ttcctggtta tcgaggactg cgtacaagac
      961 attcggatgt tgttgcgaga tcgccagtcc aatgcgccca acaccggggc ccgaaggaaa
     1021 accgctctca cctccggata tcctgttcca gtgttctcct ccatccacac ttttatagat
     1081 cgcggaagtc gcacccgact ccacaaaatt ccaggcgcga cgatcccggt gccatagagt
     1141 agcatagaga atggaagggt tgaagggatc catcaccaga tcgaccgcgc cggtggtatc
     1201 atcccccgag agcacgcgtt gccaattcgt cccgccatcg gtggtcttat aaacacctcg
     1261 ttccgcattg ggagaataca aatgacccag tgcagcaacc cagacggtct tcggatcgtt
     1321 cggatgtatc aagaccttgc cgatgtggtg cgattccggc aaaccacgct gctgccaggt
     1381 cttgccgctg tcggcgctga aaaacatccc tactcctgag tacgagctcc ggctgctgtt
     1441 cacctcaccg gttcccagcc agatgtgacg cgtcttccaa tcgaccgcga agtcgccgat
     1501 cgtcatcacg tcttcctggt cgaacagagg ctcgaacgac tgtccgttgt tactcgtatg
     1561 ccagaggccg ccggaagcat aggcgacata gaattcattc ggatcttccg ggttgacctc
     1621 cagatcgaca gcacggccgc tcatgatggt ggggccgatg ttgcgaaggg gcaggttttt
     1681 aaaaagggat tcatcggtca ggcgggtacg ccttgcaatg ccttccagac gctctttggc
     1741 aggtgtagcc ggatagatcg cttgtgcttg agcctggaca tgaacgctct gcaagagcgc
     1801 acacaccagg agaatcagtt ggttcaggtt catggtcgat tgtgaatgcc gaaaataaga
     1861 aaataagcga attttgaagc cgataaaggg ttagttacct ttcaacttta acgaatgaaa
     1921 gaatgaataa cgcagctttc cggtacggca tctggatcag catagtcctg ctcttcacct
     1981 tcctgggaac ttccctgctc tgggtggaag gatcgaatca acacctcagt gtttggctgg
     2041 gctatatcgg tttgtttgtc tcgatgatcc tcctcgtgct gggcattctt cataaacgga
     2101 ataaagagca gggcggaaag atcacatttg gacaagcatt ccggacgggc gccaccatca
     2161 cgctggctgc cggtatcctg ttctttttca tgaactgggc ctttttcgag atccgaggtg
     2221 atgcctggtt gaaaaaatac tacgctcacc aggttgaact tatccgatcc ggtgctcctt
     2281 cgcctgaggc agccgcctca cgactacaac aattcgaaga ggaatggaaa cagagcggag
     2341 aacgctataa aaacagcgca ttccaggccg gtgtcgtatt cttcaccctg gttccggcgg
     2401 gattcgtggt aagcctcgtc ggcgcctggc tcttgaaacg gacttaagta caaatcatcc
     2461 aacctctttc aacccggcca tccaatgacc gggctttttt ttgcaggcag taatatttat
     2521 ggatgatttt atggaattta attaacagta aatgaacaat ccttaagcac tcgaagtaac
     2581 acgagatctt ctgaaacccg catttaccaa gcttttccgg aggttggcca aaatttgaat
     2641 aacccattat cagaattaac ccgattaaat ctatagttat tatgaagtgt catatttttg
     2701 aaaattgata aattttagtt gaaacatttt cggatctccc accgaaactc atccagttcg
     2761 cctaccctct aactattcat accatgacac acacaccttt acatgcccat gccgggacga
     2821 gtttgaatcg cattgttcgc ctgaccagaa gtgtcagtgg cgcattgttc ttcggcttct
     2881 tcctgctgat ttcggtttca tccgcgcttg gacaaacagc cggtggcccg atcgatccgt
     2941 cgatcaagga gttgctcaat acgtatgagc acaagatcta cttcacggaa aaccgcggac
     3001 agttcaaaga gcgcgtgctc taccgggccg actttcccta cggccaggct gcggcaactg
     3061 ctgaaggcat gtacatctcc acctatgatc cggcttccgt acaggcacgc atggatgacg
     3121 gtgaattgct ggagcgcgag atccagaacg ggcaacgcat ccgacccctt aatgggcgtt
     3181 tgaaaggcca tacctggttg atgaacttcg tcggtcgctc agccgggatg acaacttccg
     3241 gcgccgaacg tcacggcgat ttctacaatt actttacggg caacacttcg cgtcacgcga
     3301 ccggtgtagc caattatcag gaagtctggt acaacaacgt ttac
//
LOCUS       DAOTON010000240         3338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22494_length_3338_cov_2.986902, whole genome shotgun sequence.
ACCESSION   DAOTON010000240 DAOTON010000000
VERSION     DAOTON010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22494_length_3338_cov_2.986902"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="P5292_05810"
     CDS             complement(<1..360)
                     /locus_tag="P5292_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRS38677.1"
                     /translation="MSDQIHQVLTRYWGFSRFRPLQEEIIRSVMDGKDTLALLPTGGG
                     KSICFQVPAMAMDGLCLVVSPLIALMKDQVERLKSKGIAAASLVSGMSKAEIDLTLDN
                     CIYGKTKFLYVSPERLGN"
     gene            complement(475..732)
                     /locus_tag="P5292_05815"
     CDS             complement(475..732)
                     /locus_tag="P5292_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38678.1"
                     /translation="MQRPYQPIDCSLLDELEWLAMRRQPVLLSYRDEQDEEIHGVALI
                     QDWYASQGIEYLVLGDGSVIRMDQLMSLNGKPVDACPSPSN"
     gene            complement(833..1675)
                     /locus_tag="P5292_05820"
     CDS             complement(833..1675)
                     /locus_tag="P5292_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRS38679.1"
                     /translation="MKKYLYTLVFLLSVSSVSTGQISSCCQKSAEAEFSALAMNERFA
                     AAHLAPEAFVLENRSGSSIEFPCLDGKSGAAYLIRSIQPTRNWLLVIHEWWGLNDHIR
                     KEAEQLQQDLVNVNVLAVDLYDGRLAETPAVAQQLMSGLEEARAKAILNGAIQYMGSD
                     ARIQTMGWCMGGGWSLKSALMAGKQSSGCVVYYGMPEKDVTRLKSLQSDVLFIFAKKD
                     AWINQAVLKEFETNMKSAGKKLTVLSYEADHAFANPSNPKFDKKAASEARKQAIAYMK
                     KHLK"
     gene            complement(1797..>3338)
                     /locus_tag="P5292_05825"
     CDS             complement(1797..>3338)
                     /locus_tag="P5292_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS38680.1"
                     /translation="HHKVRIKDEAIIAAVELSERYISDRFLPDKAIDLVDESAAKLRL
                     EIDSVPEELDELNRKIMQLEIEREAIKREKDERKLQELNEEIANLIEARTQIRAKWES
                     EKGVVQGIQQTKEQIEQLKLEAEQAERAGDYGKVAEIRYGRVREAEDRLHGLEKQLVE
                     LQAEKALIKEEVDAEDIAEVVSRWTGIPVSRMLQSEREKLLHLEDELHKRVIGQQEAI
                     AALSDAVRRSRAGLQDMRRPIGSFIFLGTTGVGKTELAKALAEFLFNDENAMTRIDMS
                     EYQERHMVSRLIGAPPGYVGYDEGGQLTEAVRRRPYSVVLLDEIEKAHPDVFNILLQV
                     LDDGRLTDNKGRTANFKNTIIIMTSNIGSHIIQEEFEGMDEWNKDEKIASAKHKVYEV
                     LKKSIRPEFLNRIDEVIMFQPLNRSEVRSIVELQFRQLQHLLAANDIRLEASDEAIDW
                     LAQLGFDPQFGARPLKRVMQKKILNELSRQILAGSVSKEDTILIDLSDKHEFLFRNQG
                     KAIEA"
BASE COUNT          770 a          900 c          809 g          859 t
ORIGIN      
        1 gtttccgagc cgttccgggg acacgtataa aaactttgtc tttccgtaga tgcagttgtc
       61 cagggtcaga tcgatctcgg ctttgctcat tccggacacc agggatgccg ctgcaatacc
      121 tttggacttc aagcgttcga cctgatcctt catcaacgcg atcaggggag atacgaccag
      181 acacaagccg tccatggcca tggccggaac ctggaaacag atcgatttcc cgccacccgt
      241 cgggagcagg gcgagcgtat ccttcccatc catgacggac cgaatgatct cttcctgcaa
      301 gggacgaaat cgggaaaacc cccaataccg ggtcagtacc tgatggatct gatcactcat
      361 gaacgataac gaaactgcaa gttacggaac atcaactgcg gtatgaaccg cttgaaatag
      421 ggaaaaagaa aaggcgccat tcctggcgcc ttggaatatc ggaggaggga agaatcagtt
      481 ggaagggctc ggacaagcgt ctacgggctt tccgttcaac gacatcagtt gatccatacg
      541 aatcacagaa ccgtcgccca ataccaggta ctcgatgcct tgagatgcat accagtcctg
      601 aataagcgct actccgtgaa tctcttcatc ttgttcgtcg cggtaagaga gcaagaccgg
      661 ctgacgtcgc attgccagcc attcaagctc atccaacaaa gaacaatcga tgggttggta
      721 ggggcgttgc atgattacaa atatagtaag attagtgatt tgagcaagaa agaagttgat
      781 aattaaagcg attgttcaga aataatattg tactgaagca tcacttcatg gcttacttca
      841 agtgtttctt catgtaggcg atagcctgtt ttcgggcttc ggatgcagct tttttgtcga
      901 atttcgggtt actgggattc gcgaaagcat gatccgcttc atagctcaaa acggtcagct
      961 ttttaccggc cgatttcata tttgtttcaa attctttcaa aacagcttga ttgatccagg
     1021 catccttttt agcgaaaata aacagtacat ctgattgcag actttttagc ctggttacat
     1081 ccttctccgg cataccgtaa tagaccacgc aacctgaaga ctgtttccca gccataaggg
     1141 ccgacttcaa tgaccagccg ccgcccatgc accagcccat ggtctggata cgggcatccg
     1201 aacccatata ctgtattgcg ccgttgagta tggctttagc cctcgcttct tcaagccccg
     1261 acatcagttg ctgcgcaacg gccggtgtct cggccaatcg gccatcgtat agatcaaccg
     1321 ccaggacatt gacgttcacg agatcctgtt gcagctgctc cgcctcttta cggatgtgat
     1381 cattgaggcc ccaccattca tggatgacga gcagccagtt cctggtaggt tggatggaac
     1441 ggatcaggta agcggcaccc gatttcccat caagacaagg gaactcgatg gaagaaccgg
     1501 atctgttctc caaaacaaag gcttcgggag ccaggtgtgc ggcagcgaac cgctcattca
     1561 tggccagggc ggaaaattcc gcttcggcgc ttttttgaca gcaggaagaa atctggccgg
     1621 tactgactga agaaaccgac aacagaaaaa cgagtgtgta cagatacttt ttcattgggg
     1681 acgatgggtc aacaaaggta cggatggtct atcgaacaac gatgacagaa taaatgttca
     1741 cagcccgcac aaagttagtt tgtccgggct gtgaactgtt ttaaaacttc ctggtctcag
     1801 gcttcaatcg cctttccctg gttgcggaac aaaaactcat gcttatcgga aaggtcgatc
     1861 aggatggtat cttccttact caccgatccg gccaggatct gacgggaaag ctcgttcaat
     1921 atcttttttt gcatgactcg cttcaacggt cgggcgccga actgcggatc gaatcccagt
     1981 tgtgccagcc agtcgatcgc ttcatcggat gcctcaagtc gtatgtcatt cgctgccagc
     2041 aggtgctgca actgccggaa ttgcaattcc acgatcgacc gcacttcgga tctgttgagg
     2101 ggttggaaca tgatcacctc atcaatcctg ttcaggaact ccgggcggat actctttttg
     2161 agtacctcgt agaccttgtg cttggcggaa gcgatctttt catctttatt ccactcatcc
     2221 attccttcga attcttcctg gatgatgtgc gaaccgatat tggaggtcat gatgatgatc
     2281 gtattcttga aattcgccgt gcgccctttg ttgtcggtca gccgtccatc gtccagcacc
     2341 tggagcagga tgttgaacac atccgggtgc gctttctcga tttcgtccag taagacgacg
     2401 gagtagggcc ggcggcgtac cgcttccgtc aattgtccgc cctcatcata ccctacgtat
     2461 ccgggagggg cgccgataag acgcgatacc atatgtcgct cctggtattc gctcatatcg
     2521 atccgtgtca tggcattctc atcgttgaag aggaactcgg caagggcttt cgccaattcc
     2581 gtctttccaa ctcccgtggt tcccagaaag atgaaggagc cgatcgggcg acgcatatcc
     2641 tgcaatccgg cacgactccg gcgcacagcg tcgctcaatg cagcgatggc ctcctgctgt
     2701 ccaatgactc gcttgtgcaa ttcgtcttcc aggtggagca atttctcccg ttcactttgc
     2761 aacattcttg aaacaggaat acccgtccaa cgagacacca cttcggcgat atcctcagca
     2821 tccacctctt ccttgatcag ggctttttcc gcctgaagtt ctaccagttg tttctccaaa
     2881 ccatgcaaac ggtcttccgc ttcgcgaact cggccgtaac gtatttccgc aactttgcca
     2941 tagtcaccgg cccgttcggc ctgttccgct tccagtttga gttgctcgat ctgctccttg
     3001 gtttgctgaa tcccctgaac gacacccttc tctgattccc actttgcgcg gatctgggtc
     3061 cgtgcctcga tcaggttggc gatttcttcg ttgagttcct gaagctttcg ctcatctttt
     3121 tcccgcttga tcgcttcccg ctcgatctcc agttgcatga tcttgcggtt aagttcgtcc
     3181 agctcctccg gtacggaatc aatttccaaa cgaagcttgg ccgctgattc gtcgaccaga
     3241 tcaatggctt tatcgggtag gaagcgatcg gaaatatagc gttcgctcaa ttccaccgca
     3301 gcaatgatcg cctcgtcctt gattcggact ttgtggtg
//
LOCUS       DAOTON010000241         3333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22552_length_3333_cov_2.877059, whole genome shotgun sequence.
ACCESSION   DAOTON010000241 DAOTON010000000
VERSION     DAOTON010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22552_length_3333_cov_2.877059"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="P5292_05830"
     CDS             complement(<1..717)
                     /locus_tag="P5292_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38681.1"
                     /translation="MAERYGYDVHDLFQRFSLMKVRADSGVRNIFARIMQYKVDYLPA
                     SEALQVVQSGQRVFIHGSAATPTHLVRALAGEAPRLKDVEIVCISVLGDFPIAESRYE
                     GNFNINSFFVSEPIRPAVNEGRADYIPVFLSEIPDLFRTGIMPLDVALVQVSEPDAHG
                     FVSLGTSVDIARAAVNTAEHVIAQVNPLMPRTHGDGLIHVSELDALVYAPEPLHETVF
                     GDRVTTTERKIGEYIAELIPD"
     gene            716..1138
                     /locus_tag="P5292_05835"
     CDS             716..1138
                     /locus_tag="P5292_05835"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRS38682.1"
                     /translation="MTADDYIRYFHMIAHPEGGYFVETFRSATNIRLADFDSDRSLAT
                     SILFLLKAGQSSALHRIKSDELWYYHDGEGLEIVEIDEQGREVVTRLGKRCFDGEVLQ
                     HVVPAGRWFGARLAAEGSFCLVGCQVSPGFDFRDFELK"
     gene            1181..1888
                     /gene="deoD"
                     /locus_tag="P5292_05840"
     CDS             1181..1888
                     /gene="deoD"
                     /locus_tag="P5292_05840"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRS38683.1"
                     /translation="MSLHINAEKGSIAPIVLLPGDPLRAKYIAENWLKDPKLVSTTRN
                     MFYFTGEYKGKRVTVGGSGMGCPSIGIYSFELYTEYQVETIIRIGTAGSYLPNLKTYE
                     LVNAEFACSESTYAHNAFGFEGNRMVHQGPAFERINEAARRLNQPVHAGTIHSNDVFY
                     RASKELPEIVAEHSCLAVEMEAFALFANARYLKKTAGTLLTISDVIPTGEVISPEARE
                     KSLKPMIELALEACVEL"
     gene            complement(1913..3316)
                     /gene="mnmE"
                     /locus_tag="P5292_05845"
     CDS             complement(1913..3316)
                     /gene="mnmE"
                     /locus_tag="P5292_05845"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRS38684.1"
                     /translation="MLNFKPDDTIAALSTAPGTGAIAVIRLSGKSAFTIGDRVFRAKS
                     RSIKSLQDQPGYSLHFGEFHFDDAVLDEVLVAVFRGPHSYTGEDTLEFSCHGSPFIQQ
                     QLLQALCQQGARLAEPGEYTLRAFLNGKLDLSQAEAVADLIASGSAAAHRVALQQMRG
                     GFSRKIKDLRAELIHFASLIELELDFSEEDVEFANRDQLRELVKRLCSQLDVLIRSFE
                     LGNVLRNGIPVVIAGKPNVGKSTLLNTLLREERAIVSDIAGTTRDTIEEELSLQGIRF
                     RFIDTAGIRDTSDTIERIGVERTLDKIRQSPLLLYLFDVTETTAGSLQLELEALRKLT
                     GSDTFRLLLVGNKSDGSKPEALEKEFKHYPEVIFLSAKSGAGLSALEDRLLSAVDTER
                     LQSGDTIVSNARHALALQQTEAALERVLAGLDSGVTHDFIASDLRVALHHLGEITGEI
                     STDDLLENIFSKFCIGK"
BASE COUNT          782 a          870 c          919 g          762 t
ORIGIN      
        1 atcaggaatc agttcggcga tgtactcgcc gatcttccgt tcggtggtgg taacccgatc
       61 gccgaatacc gtctcgtgta agggctccgg cgcataaacc agtgcatcca gttcgctgac
      121 atgaatgagt ccatcgccgt gggttcgtgg cataagtgga ttgacctgcg caatgacatg
      181 ttcggctgta ttgacggcgg ctcgggcaat atccacagag gtgccaaggg atacgaagcc
      241 atgcgcgtcc ggctccgata cctgcaccaa cgcgacatcc aggggcatga taccggtgcg
      301 gaagagatcc gggatttcgc tgagaaagac cggaatgtag tcggcacgcc cttcgttcac
      361 ggctggacgg atcggttcgg aaacaaagaa tgaattgatg ttgaagttgc cctcataccg
      421 ggattcagca atcgggaagt cccccagtac actgatacag acgatttcca cgtcctttag
      481 tcgtggagct tccccggcca gtgcccgtac aagatgggta ggagtcgccg cgctgccgtg
      541 aatgaagacc cgctgaccgg attggactac ctggagcgcc tccgaagccg ggaggtaatc
      601 tactttgtat tgcataatgc gggcaaagat attccgaacg cctgaatcgg ctctgacctt
      661 catcagggag aaacgttgga aaaggtcgtg tacatcatat ccgtatcttt cggccatgac
      721 agcggatgac tatatccggt actttcacat gattgcccat ccggagggag ggtactttgt
      781 agagaccttc cgaagtgcaa cgaacatccg cttggctgac tttgacagtg accgatcact
      841 tgccaccagc attctttttc tgctgaaagc gggtcagtcc tctgctttgc atcggattaa
      901 aagcgatgaa ctttggtatt atcacgatgg cgaaggactg gaaattgtgg aaatcgatga
      961 acagggcagg gaagtggtga cacggctggg aaagcgttgt ttcgacggcg aagtcttgca
     1021 acatgtcgta cctgcaggtc gatggttcgg ggccaggttg gcggctgaag ggtctttctg
     1081 cctggtgggt tgtcaggtca gccccgggtt cgactttagg gattttgaat tgaagtgagc
     1141 atttttgcac cccggaatat caactattga ttcaaacact atgagccttc acataaacgc
     1201 tgaaaaggga agcatcgcac ccatcgttct actcccgggc gatccgcttc gcgctaagta
     1261 catcgccgaa aactggttga aggatccaaa gctggtcagt accacgcgga acatgttcta
     1321 ctttaccggt gagtacaaag gaaagcgcgt tactgttgga ggtagcggaa tgggctgtcc
     1381 gtccatcgga atctactcct tcgaactcta taccgaatat caggtcgaga ccattatccg
     1441 gatcggaacg gccggttctt acctcccgaa tctgaagact tacgagttgg tcaacgccga
     1501 gtttgcctgc agcgaatcga cctacgcgca caacgctttc ggcttcgaag gcaataggat
     1561 ggtccatcaa gggcctgcgt ttgaacgaat caacgaagcc gctcgtcggt tgaaccagcc
     1621 ggtgcatgcc ggaacgattc attccaacga tgttttttac agagccagca aggaattgcc
     1681 cgagatcgtc gctgaacaca gctgcctggc ggtcgaaatg gaggcgttcg ccctctttgc
     1741 caatgcccgt tacctgaaaa agaccgctgg caccttgctg acaatttccg acgtaattcc
     1801 gaccggcgaa gtcatcagtc ccgaagcaag ggaaaaatca ctcaagccga tgatcgagct
     1861 ggctttggaa gcctgtgttg agctttgagt ccggactttt ccgtaaggat cgtcactttc
     1921 cgatacagaa cttggaaaaa atgttttcca ggaggtcatc ggtgctgatt tccccggtga
     1981 tctcgcccag gtgatgcaag gcgacccgca ggtcactggc gataaaatcg tgcgtaactc
     2041 cggaatcgag accggcaagt accctttcaa gtgcagcttc ggtctgctgc aaagcaagtg
     2101 cgtgccgggc gttgctcacg atcgtatccc cgctttgcaa gcgctcggtg tcaaccgccg
     2161 acaacaagcg atcttctaat gcagagagtc ccgctccgga ttttgcagaa aggaaaatga
     2221 cttcaggata gtgcttgaac tccttttcga gcgcttcggg tttgctgccg tcggacttgt
     2281 ttccgacaag cagcagacgg aacgtgtcac tgccggttag ttttcgtaac gcttcgagtt
     2341 ccaattgcag cgagccggca gtcgtttcgg taacgtcaaa caggtaaagc aggagcggag
     2401 attgccggat cttgtccagc gtacgctcca caccgatccg ttcgatcgtg tcgctggtat
     2461 cccggatccc tgcggtatcg atgaaacgaa accgtattcc ctggaggctg agctcttctt
     2521 cgatcgtatc gcgggtcgtt ccggctatgt cgctgacgat ggcgcgttct tcccgcagca
     2581 gcgtattgag gagagttgac ttgcccacat tcggtttacc cgcgataacg accgggatgc
     2641 cattccgcaa gacattgccc agttcgaacg agcgaatcaa tacatccaat tggctacaca
     2701 aacgcttgac gagttcacgc aattggtcac gattggcgaa ctcgacatct tcttccgaaa
     2761 aatcaagttc caattcaatc agggatgcaa agtggatgag ttcggcacgc aggtctttga
     2821 tcttcctgct gaaacctccg cgcatctgtt gcaaggccac gcgatgggca gcggcggaac
     2881 cactcgcgat gagatcggcg accgcttcgg cctgcgacag atccaatttt ccattcagaa
     2941 aggcgcgaag cgtatactct cccggttcag ccagccgtgc cccttgctgg caaagcgcct
     3001 gcaagagttg ttgctggatg aatggtgaac catgacagga aaattccagg gtatcttcac
     3061 ccgtgtagga gtgaggaccg cgaaaaacgg caacgagaac ttcgtccagg accgcatcgt
     3121 cgaaatgaaa ttcaccgaag tgcaggctat agcccggttg gtcctgcaac gactttattg
     3181 accgggattt tgcacgaaac acccggtcac cgatggtaaa agcggatttc cccgatagcc
     3241 ggatcaccgc aatggctccg gttccggggg ctgtcgatag cgccgcaatg gtatcgtccg
     3301 gcttgaaatt cagcatgttg aaaggagtgc aaa
//
LOCUS       DAOTON010000242         3332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22558_length_3332_cov_3.431492, whole genome shotgun sequence.
ACCESSION   DAOTON010000242 DAOTON010000000
VERSION     DAOTON010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22558_length_3332_cov_3.431492"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="P5292_05850"
     CDS             complement(<1..654)
                     /locus_tag="P5292_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38685.1"
                     /translation="MKKIFTLLAVLIGLGAQAQTILVQTDFAGYNGLPNSVPFGWFIS
                     NNDTSSTGKTFYTGASTCGVTCPAYKFNLTGAESYIITPQFSNADSVHFYMKGNGTYK
                     PNKFRVYGGADTTTWTLIQQFDSVSATSRTVSLPVGPAYNYLKFGYLKDSTGYNVGFD
                     DVIIFQGSLSSVPVTENFSANLYPNPTNGALTIDLGSLHTKSLNVTVINVIGKEVKAL
                     "
     gene            complement(747..1331)
                     /gene="pth"
                     /locus_tag="P5292_05855"
     CDS             complement(747..1331)
                     /gene="pth"
                     /locus_tag="P5292_05855"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRS38686.1"
                     /translation="MSKFLIAGLGNIGEEYTRTRHNIGFLVAERLAADLNKDETGGKL
                     FSTDRLVTIHHTRFKGKGVVVIKPTTFMNLSGKAVQYWLQAEKIPVNHLLVVTDDLAL
                     PYGTLRMKKSGGAGGHNGLTDIIDTLQTENFPRLRFGIGSEFQRGKQVDYVLGEWSED
                     EQKILNERIDQAVLMIKSFIGIGIDRTMSDFNNK"
     gene            complement(1443..1886)
                     /locus_tag="P5292_05860"
     CDS             complement(1443..1886)
                     /locus_tag="P5292_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38687.1"
                     /translation="MKKILLFALLLGGTLTASAQEFKIITIIESIVPGGMGRSRIVEN
                     QGAVNIEAITTERDGKKSNADKVDRGDLKDAADNLKETKLLNFYSLVGINFGNIASND
                     AIIAAKINEMIGKGWKVSYITSGVEADAGKDDGTGIFITRIFFTK"
     gene            2831..>3332
                     /locus_tag="P5292_05865"
     CDS             2831..>3332
                     /locus_tag="P5292_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38688.1"
                     /translation="MKHIYTLLLATLLLGVNTLRAQNAGDYRSASTGEWSTISTWETY
                     DGASWQPASAAPDGTNSGLITIQSPMTVSVTTTVPVTADELVIESGATLYIAETDFDV
                     LNGAGEDLVCQGTLYTQEGTMTNSGVVRFESGSTFGWGFNSVLSGAGTYTLDAGSAVD
                     FAATGNR"
BASE COUNT          806 a          780 c          921 g          825 t
ORIGIN      
        1 aagggctttc acttctttgc cgattacgtt gatgacggtc acgttcaggc tcttggtgtg
       61 caggctgccg aggtcgatgg tgagagcacc attggtcggg ttggggtaga ggttggccga
      121 gaaattttcg gtcaccggta ccgagctaag cgaaccctgg aagatgatga catcatcgaa
      181 gccgacgttg taaccggtgg aatctttcag gtagccgaac ttgaggtagt tgtaagccgg
      241 accgacgggc aggctcacgg tgcgggaagt tgcagagacg gaatcgaact gctggatcag
      301 cgtccaggtg gtggtgtcgg caccgccgta cacgcggaac ttattcggct tgtaggtgcc
      361 gttgcccttc atgtagaagt gaacggagtc ggcattgctg aactgcgggg tgatgatgta
      421 ggattcagcg cccgtcaggt tgaatttgta agccggacag gtcactccac aggtgctggc
      481 gccagtgtaa aaggttttac cggtcgagct cgtgtcgttg ttgctgatga accagccgaa
      541 aggaacggaa ttcggaaggc cgttgtagcc tgcaaagtcg gtttgaacga gaatggtttg
      601 agcctgtgcg cccagaccaa ttagtactgc aagtagcgta aagatctttt tcatcgaatt
      661 tggattgggg gataaaatta agcattccca cgatgccgga aaaggggcgg cccgttatgg
      721 aaatgtcaag tattggcgag aaggccttat ttattattaa aatccgacat cgtacggtca
      781 atgccgatgc cgatgaagct ttttatcatc aaaacagcct gatcgatccg ctcgttcagg
      841 atcttttgct cgtcttcgct ccattcgccc aaaacataat ccacctgttt cccgcgttga
      901 aactcactac cgatcccgaa ccgaagccgg gggaagtttt cggtttgaag cgtatcgatg
      961 atatcggtca aaccgttgtg gccaccggcg ccgccgcttt ttttcatccg taaggtgccg
     1021 taaggcaggg ccaggtcgtc ggttaccacg agcaggtgat tcaccgggat cttttcggcc
     1081 tgcaaccagt actgcacagc cttgccgctc aggttcatga aggtggtggg cttgatgacg
     1141 accacgccct tccctttgaa ccgggtatga tggatcgtaa cgaggcggtc ggtagagaac
     1201 aatttcccgc cggtttcatc cttgttcagg tcggcagcca ggcgttcggc aacgaggaag
     1261 ccaatgttgt gccgtgtgcg cgtgtactct tctccgatgt taccgagtcc ggcgattaag
     1321 aacttactca tgacgattgc aggtggccga caaagatagt caggtcgccc gatggttgtg
     1381 ataactaaaa aagcctgtcg caccttgagt ggcgacaggc tttgaaaaga tgtgggaacg
     1441 gattacttcg taaagaagat gcgggtaatg aagataccgg taccgtcgtc cttgccggca
     1501 tcggcttcga cgccgctggt gatgtaggag actttccagc ctttgccgat catctcgttg
     1561 atcttggcgg cgatgatcgc gtcattcgaa gcgatgttgc cgaagttgat accgacgagc
     1621 gagtagaagt tgagcagctt ggtctctttc aggttgtcag cagcatcctt gagatcgcca
     1681 cgatccactt tgtcggcatt gcttttcttg ccgtcacgtt cggtggtgat ggcctcgatg
     1741 ttcacggcgc cctgattttc cacgatgcgg gaacgtccca taccgccggg tacgattgat
     1801 tcaatgatgg tgatgatctt gaattcctgt gcggaagcgg tcagggtgcc gcccagcaga
     1861 agtgcgaaga ggaggatttt tttcataagg gttagttgca actggtttat tcgccgaagg
     1921 taggcagata atggaagttc gcgcctgcgg tccgacagac agtcggaata accggatgaa
     1981 acggatgatt gattctttga aaggaaaagt cgattgtctg ccatcagcca tcagttggag
     2041 gaagtcacgc gcacaagccc ggcaaatcag gtatgacgtt tgggtattat caaacaggct
     2101 ctttcctgcg gagtaccgca acaacggacc ctcgatcagg agctagtatg gcccgtaggc
     2161 gggtcttcat tagttcgatc taccgtgaat tcaccgcgcc ggggttggct aaacgatgtt
     2221 atatagctga aatacagcgc aatacagaaa ttttaacgaa ttcgatacgg gtcagccagt
     2281 tactttgatt tttataagtg attgaaaata agtaaaataa atgctgtatg cggtttccag
     2341 cgtctgcctg tcggggtaag acaagtgctg gatgatttct tggattccag gtttacaact
     2401 gatcattaac cacgaaaagg gcattattaa ccaatttaac attaaaaatg accaaattcg
     2461 aggtataaag ttgaattgat aatctaacat tcacccaaaa atgttttgat tttcatgcat
     2521 tttggatata ttgaacccct attgatgttt taatgtggcg gttgattggg acatggcttc
     2581 taatcatgtc ctgttgctat aaaaaatgat tgtaaaaggt atttaacatt tatccatgtc
     2641 agcaaacaca cagcatcgcg gagtgagcaa ctccgatatc atctattcat aacaagaacc
     2701 aaagtacctg acgctgcatc ctagtttgaa ccgacgatga tgcgacgaca ggaagtacca
     2761 tgccgatcat ctgctgagac ggagcagaat cggatggaat gaactattgt aacacacaca
     2821 caaccaatac atgaagcaca tctacaccct gttgcttgcc acgctgctgc tcggagtcaa
     2881 cacccttcgc gcgcagaacg ccggagacta ccgcagcgca tccacgggcg aatggtctac
     2941 gatctccact tgggaaacct acgacggcgc ttcctggcag ccggcctcgg ctgctccgga
     3001 tggcaccaac agtggcctga ttacgattca aagtccgatg acagtatcgg tgaccactac
     3061 ggttccggtc accgccgacg aactggtcat tgaatcgggt gctacgttgt acatcgcgga
     3121 aacggatttt gatgtcttga atggagcagg tgaggacctg gtctgtcagg gaaccctcta
     3181 tacccaggag ggaaccatga cgaacagcgg cgtggtacgc ttcgaatcgg gttccacctt
     3241 cggctgggga ttcaactccg tactcagcgg tgccggaacg tataccctgg atgccggctc
     3301 agcggttgat ttcgccgcga cgggtaacag ga
//
LOCUS       DAOTON010000243         3318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22684_length_3318_cov_3.089488, whole genome shotgun sequence.
ACCESSION   DAOTON010000243 DAOTON010000000
VERSION     DAOTON010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22684_length_3318_cov_3.089488"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1207)
                     /gene="alc"
                     /locus_tag="P5292_05870"
     CDS             complement(188..1207)
                     /gene="alc"
                     /locus_tag="P5292_05870"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004737479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HRS38689.1"
                     /translation="MNQHDAPAFTRLINLAAAQLGAEALLCTDDFFAEKENLVKSGRG
                     IFIPDKYTDRGKWMDGWESRRKRVPGHDWCIVRLGAPGIVRGFDIDTNHFLGNHPPHA
                     SIEACYRPDFPDAAVLDSAAVEWKEILPKSPLQPGSQNFYACDHQQLVTHVKLHIYPD
                     GGVARLKVYGEVKKDWSKVGSNDRVDLASALNGAKAILCNDMFFSHMDNLLMPGRGVN
                     MGDGWETKRNRTPNNRDWVIIRLAHPGTIDEILVDTCHFKGNYPDGCSLEGCYLPEGK
                     DPDLADDKIQWTELLPRQKLQADHEHTYKSEIKQHQRISHVRLYIYPDGGVSRLRLFG
                     TIGKP"
     gene            complement(1204..2556)
                     /gene="allB"
                     /locus_tag="P5292_05875"
     CDS             complement(1204..2556)
                     /gene="allB"
                     /locus_tag="P5292_05875"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020406403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HRS38690.1"
                     /translation="MEAEKAIHSKRVLLPDGIRDVTLLIGNGTILSIEPGKWSAPKGF
                     PFIEAGESLIMPGLIDSHVHINEPGRTDWEGFESMTKAAAAGGITTLVDMPLNSSPVT
                     TNTKALQAKLDAAKGKLMVNVGCYGGIVPGNSDQLEALAKAGVLGFKVFLTHSGIDEF
                     PETSMKELDRVAPILAKLGLPLLAHAELDNSHPGQAELEDDHYSYNAYLHSRPNNWED
                     EAVRQLIALCEKHKVRTHIVHLSSASALPMIREARAKGLPLTAETCPHYVYFSSEKIP
                     DADTRFKCAPPIRDAANNQLLWDALTDHTLDFIVTDHSPAPPETKELDSGDLKAAWGG
                     IASIQFSLPVVWTLAKQRKNTLEEVAAWMSTRVADFLGLPKKGRIQVGADADLVIWSP
                     DKKMKINRNNIQFRHKVTPYEGEMVFGVVEQTWVNGELAYDRGGFVSSPPGKILLHQA
                     "
     gene            complement(2563..>3318)
                     /locus_tag="P5292_05880"
     CDS             complement(2563..>3318)
                     /locus_tag="P5292_05880"
                     /inference="COORDINATES: protein motif:HMM:NF017945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRS38691.1"
                     /translation="LLERKSLFAVIGFMIVVLLAWLLSRYLSGRAAFMHVGALLGTIM
                     AGNVFFVIIPAQKALVAAARRGQTPDPTLGKFAGLRSLHNNYITLPVIYVMISNHFPV
                     TYGGAWNWAILAGLTLGSVAVRHYINLHEKGIHAASMLPLAAIALFALIVVTAPKPST
                     TGGKPVSFSQVAPIFQERCVSCHSAKPTDDVQKIAPNGVMFDTPDQIKKMADRILVRA
                     VQTHTMPQANKTGMTEAERQLIGDWINQGASLQ"
BASE COUNT          647 a          868 c         1016 g          787 t
ORIGIN      
        1 ttccagccag tcggatgccg ttgtggtgga ccatgcctgc tccgcaacct gcaagcaacc
       61 ggctgtgtcg cgaaatgggc gggcagccag catgagcgac acccagcgct gcgcgccaca
      121 gcaattgtgc agcgtctggc gggcttcatt ttccggcaaa ctattgaggg cttcgagcga
      181 catgtgatca gggcttccca atagttccga agagtcgcag acggcttaca cctccgtccg
      241 gatagatata caggcggacg tgcgagatcc gctggtgctg tttaatttcc gacttgtagg
      301 tatgttcgtg atccgcctgc agcttttgtc gtgggagcaa ttcggtccac tggatcttgt
      361 cgtcagctaa atcggggtcc ttgccctccg gcagatagca gccttccagc gaacagccat
      421 cggggtagtt tcctttgaag tggcaggtgt ccaccagaat ttcatcaatc gttccgggat
      481 gtgccagtcg gatgatgacc caatcgcggt tgttgggtgt tcggttgcgt ttggtttccc
      541 atccgtctcc catgttcacc ccgcggccgg gcatgaggag gttgtccatg tgggagaaga
      601 acatgtcgtt gcacaggatc gccttggcgc cgttcagggc ggaagccagg tcgacgcgat
      661 cattactgcc gactttgctc cagtcctttt tcacctcccc gtacactttg agtcgggcga
      721 ctccgccatc gggatagatg tgcaacttga cgtgcgttac caactgttgg tgatcgcagg
      781 cgtaaaaatt ctgactgccc ggttgaagcg gggacttggg taagatctct ttccattcca
      841 cggcggcgga atcaagaaca gcggcatcgg ggaagtcggg gcgatagcag gcttcgatgg
      901 aagcgtgtgg tggatggttg cccaggaagt ggttggtgtc gatgtcgaag ccgcgtacga
      961 tgcccggcgc gcctaagcgg acgatgcacc agtcgtggcc gggaacacgt ttcctgcgcg
     1021 actcccaacc gtccatccac ttgccgcggt cggtgtattt gtcggggatg aagatgccgc
     1081 gtcccgattt caccagattt tccttctcgg cgaaaaagtc gtcggtacac aatagcgctt
     1141 cggcgcccaa ttgggcggcg gccaggttga tcagtctggt aaaggccggt gcgtcgtgct
     1201 ggttcatgcc tggtgtagaa gaatttttcc gggtggcgaa gatacgaacc cgccacggtc
     1261 gtatgccagt tcgccattaa cccaggtttg ctcaaccacg ccgaaaacca tctcgccttc
     1321 gtagggggtg actttgtgac ggaactggat gttgttccgg ttgatcttca ttttcttatc
     1381 cgggctccag atgaccaggt ccgcatcggc tccgacctgg atgcgacctt ttttaggcaa
     1441 gcctaaaaaa tcggctaccc gtgtactcat ccaggcggcg acctcttcca gggtattctt
     1501 gcgttgtttg gccagcgtcc aaacgaccgg taaggagaac tggatggagg caatgccgcc
     1561 ccaggccgct ttcagatcgc cggagtcgag ctctttggtt tccggaggag cgggtgagtg
     1621 atcggtgacg atgaagtcga gtgtgtgatc cgtcaaggca tcccacaaca gctggttgtt
     1681 cgcggcatcg cggatcggtg gcgcacattt gaagcgcgtg tcggcgtcgg ggattttttc
     1741 cgaggagaag tagacgtagt gcggacaagt ttctgcagtg agcggcaagc ctttggcgcg
     1801 cgcctcgcgg atcatcggta aagccgaggc ggaagaaagg tggacgatgt gcgtccgaac
     1861 tttgtgtttt tcacataagg cgatcaactg tcgtaccgct tcatcctccc agttgttggg
     1921 tcgtgaatgc aagtaggcat tataggagta gtggtcgtcc tccaattccg cctgacccgg
     1981 atgggagttg tcgagttccg catgggccaa caagggcagg ccgagtttag ccaggatcgg
     2041 cgcgacgcgg tcgagttcct tcatcgaggt ttccggaaat tcatcgatcc cggaatgtgt
     2101 gaggaatacc ttgaaaccaa gcacaccggc cttggcgagt gcttcaagtt gatcggaatt
     2161 gcccggtaca atgccgccgt agcatccgac gttcaccatc agttttccct ttgcggcatc
     2221 gagcttggct tgtagcgctt tggtgttggt ggtgacgggt gaggagttca gcggcatatc
     2281 caccagtgtg gtgataccgc ctgctgcagc ggctttggtc atggactcaa agccttccca
     2341 gtcggttcga cccggctcgt tgatgtgtac gtgcgaatcg atgagtccgg gcatgatgag
     2401 ggattcgccg gcttcgatga acgggaagcc tttcggtgcg gaccatttcc cgggttcgat
     2461 cgacaggatc gttccattcc cgatgagtaa ggtcacgtcc cggataccgt cgggcaggag
     2521 cacccgttta ctgtgaatgg ctttttcggc ttccatgttt tcttattgta aggaggctcc
     2581 ctggttgatc cagtctccaa tgagttgacg ttccgcctcc gtcatgccgg tcttattagc
     2641 ctggggcatg gtatgcgttt gcacggcacg tacgagaatc cggtcggcca tttttttgat
     2701 ctgatccggg gtgtcgaaca tgaccccatt cggcgcgatt ttctgtacgt cgtcggtcgg
     2761 cttcgcggaa tgacaggata cacaacgctc ttgaaagatg ggagcgacct gagagaagga
     2821 tacaggctta ccgccggttg ttgaaggctt cggggcggtc acgacaatca gcgcgaataa
     2881 ggcgatggcg gcaagtggta acatgctggc tgcatggatt cctttttcat gcagattgat
     2941 gtagtgacga accgccacgc ttcccagggt cagacccgca aggattgccc agttccaggc
     3001 gcctccgtag gtgacgggga agtggttgct gatcatcaca tagatgaccg gcaaggtgat
     3061 gtagttgtta tgcagcgagc gaaggcccgc aaattttccc agcgtcgggt cgggtgtttg
     3121 tccgcgacgc gccgcggcta ccagcgcttt ctgggcagga atgatcacga agaagacatt
     3181 gccggccatg atggtgccca gcagcgcgcc gacgtgcata aaggctgctc gtccgctcag
     3241 gtagcgggac aagagccagg ccagtaagac cacgatcatg aagccgatta ctgcaaacaa
     3301 gctctttctt tccagtaa
//
LOCUS       DAOTON010000244         3304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22811_length_3304_cov_2.885195, whole genome shotgun sequence.
ACCESSION   DAOTON010000244 DAOTON010000000
VERSION     DAOTON010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22811_length_3304_cov_2.885195"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="P5292_05885"
     CDS             <1..492
                     /locus_tag="P5292_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38692.1"
                     /translation="RRTVPGNDANFEECVFAAVGRNVTNEAVVLYQRDGAPGHALATA
                     GTCDQANNLGNASDIVYTLVDALTVGTDNSIANNNFSVSQNYPNPSNGSTSINITLKK
                     ASSINFTVADILGKVVYTEQLDNLAAGIHTVTLNTGSWAHGVYTYTVRTADNAVTKQM
                     IVK"
     gene            complement(573..1289)
                     /gene="bshB1"
                     /locus_tag="P5292_05890"
     CDS             complement(573..1289)
                     /gene="bshB1"
                     /locus_tag="P5292_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HRS38693.1"
                     /translation="MKLDVLAFGAHPDDVELSCAGTILQLVRSGRKVGVVDLTRGELG
                     TRGTPELRDQEAREASRILGLSCRENLGMRDGFFENDPEHQLQVVRMIRRYQPELILC
                     NAVSDRHPDHGRGAALVAKAAFLAGLLKVETDDNGASQAPWKCRALYHYIQDRYDTPD
                     FVVDVAAVWKERMEAVLAFKSQFYNPESNEPETPISSREFLAYLEAKAAVFARPAGLT
                     YAEGYIASRTPAVEDIFDLL"
     gene            1367..2773
                     /locus_tag="P5292_05895"
     CDS             1367..2773
                     /locus_tag="P5292_05895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS38694.1"
                     /translation="MKITFHGAARTVTGSKHLLELEDGRKILLDCGMFQGHGADTDPL
                     NRHLGFDPKELSHVILSHAHIDHSGLLPLLAKEGYEGNIYCTPATRDLCAIMLADTAH
                     IQEYDSVYINRKRKQNGLPGVKPIYRMEHVEAVMQQFCTVPYEKTYKVDDGLELRFTD
                     SGHILGSAAVNLIIREKDKVTRLCFTGDIGRPDDLLLRTPSPFPQPDVLICESTYGNR
                     KHEDREISQKRLLEIVLETCIQRSGKLVIPAFSLGRTQELVYALNNLHNAGLLPPIPV
                     YVDSPLSVNATVIMRAHMECFNNELRKLLQNDPDPFGFDQLHYIQRAEDSMKLNEMKE
                     PMIILSASGMAEAGRTKHHIRNTIESAQNTILLVGYCTPDSLGGRLGAGAKEVRIFGK
                     PYSVNARVEQLNAYSAHADYEEMLQYLSCIESRKVKHTFLVHGDYDVQHDWRQKLEAA
                     GFGNIEIPDMHSSWEIPA"
     gene            2770..>3304
                     /locus_tag="P5292_05900"
     CDS             2770..>3304
                     /locus_tag="P5292_05900"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HRS38695.1"
                     /translation="MKRQLTALIAKEVRLEWKQRYALGGILLYVIATVFIVYLSFRQV
                     IDLPTWNALFWIIQVFAAVNAIAKSFLQESRGRMLYYYTLADPRAVILSKTIYNLGLM
                     FLLSVINLACYSLFIGNPVQDILQFTIAVLLGSLGFAAVLSMVSAIASKAGNNTTLVA
                     ILGFPILIPLLITTIRFS"
BASE COUNT          797 a          921 c          837 g          749 t
ORIGIN      
        1 cgtcgtaccg ttccgggcaa cgatgccaac ttcgaagagt gtgtcttcgc tgctgttggt
       61 cgtaacgtaa ccaacgaagc cgtggtactt tatcagcgtg acggcgctcc tggtcacgcg
      121 cttgcaactg caggtacctg cgatcaggcc aacaacctcg gtaacgccag cgatatcgtt
      181 tacaccctgg tagacgcgct gacggtcggc accgacaatt cgatcgccaa caacaacttc
      241 tcggtttctc agaactaccc gaacccgtcg aacggatcga cctcgatcaa catcaccttg
      301 aaaaaggctt cgtcgatcaa cttcaccgtt gctgacatcc tcggcaaagt agtttacacc
      361 gagcaactcg acaaccttgc tgcaggtatc cacaccgtta ccctgaacac cggttcatgg
      421 gctcatggtg tttacaccta cacggtacgc acggccgaca acgctgtaac caagcaaatg
      481 atcgtgaagt aatacccggg gaatactacc aactccccaa aataaaaacg ccccgtccta
      541 ggacggggcg tttttatttt aaagcaggaa tcttacaaca aatcaaatat gtcctctacg
      601 gcaggggtcc gggacgcgat gtaaccttcc gcataggtca agccggcagg gcgggcgaat
      661 acggcagcct tggcttcgag ataggcaaga aactcacggg aagagatggg tgtttccggt
      721 tcattggatt ccggattata aaactgggat ttgaaggcaa gtactgcttc catccgctct
      781 ttccagacag cagctacgtc gacgacaaaa tcgggggtgt cgtagcggtc ctgtatgtaa
      841 tgatacaagg ctctgcattt ccagggcgcc tgacttgctc cgttgtcgtc tgtctcgacc
      901 ttcagcagac cggcgaggaa ggcagccttc gcgaccagcg ccgcaccccg accatggtcc
      961 ggatgccgat cggaaacagc gttgcagagg atcaactcgg gttgatacct ccggatcatc
     1021 cggacgactt gcagttgatg ttccggatca ttttcgaaaa aaccatcccg catacccagg
     1081 ttctcgcgac aactgagacc cagaattcgg gaggcttccc gggcttcctg atcccgtagt
     1141 tcaggggttc cacgggtacc cagttcaccc ctcgtcagat ccacgacacc gaccttacga
     1201 ccggaacgga cgagttgcag gatggtgccg gcacaggaca attccacatc gtccggatga
     1261 gcgccaaaag caagcacatc taatttcatg gttcaaatgt agtaaatggg gatattcccc
     1321 ttccccggcc taattcttcg taatttcgat gcaccaattg aaatccatga agatcacttt
     1381 tcacggtgca gccagaacgg tcaccggttc caaacatctg ctagagctgg aagacggtcg
     1441 gaagatcctg ttggattgtg gcatgttcca gggacatggt gccgacacgg accccctcaa
     1501 ccggcatctg ggattcgatc cgaaagagct gtctcacgtc attctttccc atgcgcacat
     1561 tgaccattcc ggccttctgc ctttgttggc caaggaaggg tatgaaggca atatttactg
     1621 cacgcccgct acgagggatc tctgtgccat catgctggcg gatacggcac acattcagga
     1681 gtacgactcc gtttatatca accggaaacg aaaacagaac ggacttcccg gagtgaagcc
     1741 gatctaccgg atggaacatg ttgaggcggt catgcagcag ttttgtacgg tcccctacga
     1801 gaagacctac aaagtggatg acgggctgga acttcgcttc accgattcag gacatatcct
     1861 gggttcagcg gcggtaaacc tgatcattcg ggagaaagat aaagtcaccc gcctgtgttt
     1921 cacaggtgat atcggacgtc cggacgacct gctacttcgt acgccttccc catttccaca
     1981 accggacgta ctgatctgcg aatcgaccta tggaaaccgg aagcacgaag accgggagat
     2041 ctctcaaaag cggttgctgg aaatcgttct tgagacctgt atccagcgaa gcgggaaatt
     2101 ggtgatcccg gcttttagtc tgggaagaac acaggaattg gtctatgcgc tgaataatct
     2161 gcacaatgcc ggcctcctgc cgcccatccc ggtttacgtc gacagcccct tgtcggtcaa
     2221 tgcgaccgtt atcatgcggg cgcacatgga atgcttcaac aacgaacttc gcaaattgct
     2281 acagaacgat cccgatccgt tcggattcga tcagttgcat tacatacagc gcgcagaaga
     2341 ctccatgaag ttgaacgaga tgaaggagcc catgatcatt ctttcagcat ccggaatggc
     2401 ggaagcagga aggaccaagc atcacatccg gaatacgatc gaatccgcgc aaaacaccat
     2461 tctcctggtc ggttattgca caccggacag tctgggtggc cgcctgggtg ccggcgcaaa
     2521 ggaggttcgg atcttcggca aaccctattc ggtcaacgcc cgtgtcgaac agctcaacgc
     2581 gtactctgct cacgcggatt atgaagaaat gttgcagtac ctgtcgtgca tcgaaagccg
     2641 aaaggtgaag catacatttc tggtacacgg tgattacgat gtacaacacg attggcggca
     2701 aaaactggaa gctgcgggtt tcgggaacat tgaaattccg gacatgcaca gttcctggga
     2761 gatccctgca tgaagcgaca actcaccgcg ctgatcgcga aagaagtcag gctcgaatgg
     2821 aagcagcggt acgcgctggg cggcatcctg ctctatgtga tcgcgaccgt attcatcgtt
     2881 tatttgtcct ttcgccaggt cattgacctg ccgacctgga acgccttatt ctggatcatt
     2941 caggtttttg ccgcggtcaa tgccattgca aagagtttcc tgcaggaatc gcgcgggcgt
     3001 atgctctact attacacgct ggccgatcca cgagcggtca tcctttccaa gacgatttac
     3061 aacctcggat tgatgttcct cttgtcggtc atcaacctgg catgttactc gcttttcatc
     3121 ggcaatccgg ttcaggacat cttgcaattt accattgccg tcctgctagg cagcctggga
     3181 ttcgcggcag tcctatcgat ggtttccgcc atcgcttcaa aagcgggcaa taacacgaca
     3241 cttgttgcca tcctgggctt tccgatcctc atcccgcttc tgatcacgac cattcgtttt
     3301 tcaa
//
LOCUS       DAOTON010000245         3295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22891_length_3295_cov_3.724074, whole genome shotgun sequence.
ACCESSION   DAOTON010000245 DAOTON010000000
VERSION     DAOTON010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22891_length_3295_cov_3.724074"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="P5292_05905"
     CDS             <1..863
                     /locus_tag="P5292_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38696.1"
                     /translation="DRSRINRWTYWNEQGEQIADSLFVRHTPLSVQPSPGTVEDSLEI
                     RAHVGGQWIPVRSSGLAMEPDCFLPDSADSIRVSNRYGSAVVPIHYPRGIGEGLQVHL
                     LKPGQRYYYFHRNRMPIDFLQNSFYVECKMDSGEGRHTSLALIEELKHAFPGGQWQRH
                     PYRNNLWRIDLPMAGVVAASDLARHPLVEHVFNLVGGEYPLDPPSYLNGYVYFQLAIG
                     VQPDSIDRILTRHGFKRSAASSPEYFGAQYDAKLVTDQFLDDYNRLAEELELSNSYPM
                     IMAYIRQEPE"
     gene            965..1357
                     /locus_tag="P5292_05910"
     CDS             965..1357
                     /locus_tag="P5292_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38697.1"
                     /translation="MPLTDADRNEIISRLNKSMAKVCPPMIQKSASDKGMEIIGNKPV
                     PYGYKKEIIPGMYFASSVARKDMVSFYFFPCYTQAKALGALAPKLMKCLKGKTCFNIK
                     KVDQVDEKEVTALLKAGVKIWKEMGYMK"
     gene            complement(1359..2525)
                     /locus_tag="P5292_05915"
     CDS             complement(1359..2525)
                     /locus_tag="P5292_05915"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020597396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRS38698.1"
                     /translation="MSFKPRHIAILGSTGSIGTQALDVVREQQGRFVVEVLSSNNKVD
                     LLIEQAREFQPNAVVIGDTRHDRALRDALADLPIKVFSGADALEQVVQMDGIDMVLTA
                     LVGYAGLKPTLSAIRAGKHIALANKETLVVAGELVTALAKEKAVNIYPVDSEHSAIFQ
                     CLAGEWHNPIEKIILTASGGPFRGRTRAELAHVTKAQALKHPNWDMGAKITIDSASLM
                     NKGLEVIEAKWLFNLKPEQIEVVVHPQSIIHSLVQFEDGSIKAQLGLPDMKLPIQYAL
                     AYPERLRNSFPRFDFSRYPAFTFEQPDTETFRNLALAIDAMRKGGNMACIVNAANEVA
                     VAAFLEDRIGFLEMSDLIERCMRQVSFIAHPTLEDYVKTDAETRRLALEGLPVR"
     gene            2642..>3295
                     /locus_tag="P5292_05920"
     CDS             2642..>3295
                     /locus_tag="P5292_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38699.1"
                     /translation="MKYLFCSITLCIAVLSAAAQAPFSSWYAISYESPAYIPTFEKVD
                     LLTGNRVLSVPLPYAGYFSDVHFFSRPGRYVVQLQDSLQDNYLVEIDTNGIVVRNTYI
                     DSGPGTLTKYILPSFNSNFYYGLRLSSTAPGYEFFRLNPQSGLTTVLATMSNTGTVYA
                     DNAVINRRDELFFWSNDFMAGGDVLYRAEPSTQSIVALDTLPGPDRFFASIFDCYTST
                     "
BASE COUNT          696 a          923 c          890 g          786 t
ORIGIN      
        1 aggatcgttc gcgtattaat cgttggacct attggaacga acagggcgag cagatcgccg
       61 atagcctgtt cgttcggcat acacccttat cggtacaacc aagcccgggc accgttgaag
      121 attcgcttga gattcgtgca cacgtaggcg gacaatggat cccggttcgt tcgtccggac
      181 tggctatgga gccggattgt ttccttcctg attcagcaga ctccatacgt gtgtccaacc
      241 ggtacgggtc cgctgtcgtt ccgatccatt acccccgcgg tatcggtgaa ggcttgcagg
      301 ttcatttgtt gaagccgggg cagcgatact attactttca tcgaaacagg atgccgatcg
      361 actttttgca gaacagtttt tatgtcgaat gcaagatgga ttccggcgaa ggccgacata
      421 cttccctcgc cctgatagaa gaactgaaac acgctttccc gggtggccaa tggcaacgac
      481 atccttatcg taacaacctt tggcgaatcg atcttccgat ggcgggcgtt gttgcggcaa
      541 gcgatctggc ccggcatcca ctcgttgaac atgtcttcaa ccttgtcggc ggtgaatacc
      601 cattggatcc gccttcgtat ctgaatggct acgtttactt ccagctcgct atcggtgtcc
      661 agccggattc catcgaccgg atcctgacac gacacgggtt caagcggtcc gctgcttcca
      721 gtccggagta tttcggagct cagtacgatg ccaaactggt gaccgatcaa ttcctggacg
      781 actacaatcg tcttgcggaa gagcttgaac tgtcgaactc ctatccgatg atcatggcct
      841 acatcaggca ggagccggaa taggggtcgt ccgcatgacc tttttgcttc tcctgaagtg
      901 tatgcgctag tacgcgggta ggacaggatt ttatactttc accattatta caaaacgaat
      961 tatcatgccc cttaccgatg ccgaccgcaa cgagatcatt tcccggctca acaaatccat
     1021 ggcgaaggtt tgtccgccta tgatccagaa gagcgcttcc gacaagggaa tggagatcat
     1081 cgggaacaaa ccggttccgt acggttacaa gaaggaaatc atccccggga tgtactttgc
     1141 ctcttccgtt gccagaaaag acatggtcag cttttatttc ttcccttgtt acacacaggc
     1201 caaagcgctg ggcgcactcg ccccgaagct gatgaagtgc ctcaaaggga aaacctgctt
     1261 caacatcaag aaggttgatc aggtggatga aaaggaagtg acggctttgt tgaaagccgg
     1321 tgtcaagatt tggaaagaga tggggtatat gaaataattc agcgcaccgg taatccttcc
     1381 agcgcaagcc ggcgcgtttc cgcgtcggtc tttacatagt cttccagtgt cggatgagca
     1441 atgaacgaga cctgccgcat gcagcgttcg atcaggtcgc tcatttccaa aaaaccgata
     1501 cggtcttcga ggaacgcggc caccgcgact tcgttcgcgg cgttcacgat acaggccatg
     1561 ttaccccctt tgcgcatcgc gtcgattgcc agcgccagat tgcggaatgt ttcggtatcg
     1621 ggctgctcga aggtgaaggc ggggtagcgg gaaaagtcga agcgggggaa tgaattcctt
     1681 aaacgctccg gatacgcgag cgcgtattgg atcggcagtt tcatgtcggg cagaccaagc
     1741 tgtgccttga tcgatccgtc ttcgaactgg accagcgagt ggatgatgct ttgcggatgt
     1801 accacgactt cgatctgctc cggcttcagg ttgaacagcc acttcgcttc gatcacttcc
     1861 agtcctttgt tcatgagcga agccgagtcg atcgtgatct tcgcgcccat gtcccagttg
     1921 ggatgcttga gggcctgcgc tttggtcaca tgtgccagtt cggcacgagt gcgtccacgg
     1981 aacggtcccc cggaagcggt gaggatgatc ttctcgatcg ggttgtgcca ctctccggcc
     2041 aggcattgaa agatcgccga atgttcggag tcgacaggat agatgttcac cgccttctct
     2101 ttcgccagcg ccgtgacgag ctcgcctgct accaccagcg tttccttgtt cgccagggcg
     2161 atgtgcttgc cggcacggat cgcgctcagc gtcggcttca gtccggcgta accgacgagc
     2221 gcggtcagca ccatgtcgat cccgtccatt tgcacgacct gctcgagggc atcggcaccc
     2281 gagaagacct tgatcggcag atccgccagc gcgtcgcgca gcgcacgatc gtgccgggta
     2341 tcaccgatca ccaccgcgtt cggttgaaac tcccgcgctt gttcgatcag caggtcgacc
     2401 ttgttgttcg agctcagcac ttccaccacg aagcggccct gttgttcgcg tacgacgtcc
     2461 agcgcctggg tcccgatgga accggtggag ccgaggatgg caatgtggcg aggcttgaaa
     2521 gacacgggaa acccttgtac tttaacgtgg acgacgaaat taacgctttt tacgccgctt
     2581 tgttcaggcc attcacaaaa ctccggtagt gggaattcgg gcaggctttt atattggcga
     2641 aatgaaatac ctcttctgct ccattaccct ttgtattgcc gtactcagtg ctgccgcgca
     2701 agcacctttc agctcctggt atgcgatttc gtacgagtcg cccgcgtata tccccacctt
     2761 tgagaaggtg gacctgctca cgggcaaccg ggtactcagt gttccattgc cctatgcggg
     2821 ttatttcagc gatgtgcatt tcttcagccg tcccggtcga tatgtcgtgc aattgcagga
     2881 cagtctgcag gataactacc tcgtcgagat cgatacgaac ggcatcgttg tacgaaatac
     2941 gtacatcgac agcggacccg gtaccctgac caaatacatc ctcccttcgt tcaactccaa
     3001 tttctattac ggcttgcggc tgtccagtac tgcgccgggt tacgagttct ttcggctcaa
     3061 tccgcaatcg ggcttgacga cggtgctggc gacgatgtcc aacaccggga ccgtgtatgc
     3121 cgataacgca gtcatcaacc ggcgcgacga attgttcttc tggtcgaatg atttcatggc
     3181 cggcggagat gtgctgtacc gcgccgagcc gtccacgcaa tccatcgtgg cgctcgatac
     3241 ccttccgggg cccgatcgct tcttcgcgag catcttcgat tgctacacca gtacg
//
LOCUS       DAOTON010000246         3291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22928_length_3291_cov_3.048826, whole genome shotgun sequence.
ACCESSION   DAOTON010000246 DAOTON010000000
VERSION     DAOTON010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22928_length_3291_cov_3.048826"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="P5292_05925"
     CDS             <1..456
                     /locus_tag="P5292_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRS38700.1"
                     /translation="KVVSNGNGYVKFPINEPATGRKKSQVEEYLDFYEGAGCQHIAVA
                     TDDIIFTVSELRKRGVEFLYVPGSYYDTVPERVGKIKEDLEVLKGLGIMADRDEEGYL
                     LQIFTKPVEDRPTLFFEIIQRVGAKSFGKGNFQALFESIEAEQARRGTL"
     gene            520..1257
                     /locus_tag="P5292_05930"
     CDS             520..1257
                     /locus_tag="P5292_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRS38701.1"
                     /translation="MQPSAANKKQLVEPFYELSCHNCPVLDCSVLRHCTQDCLEKIDK
                     LKKVVGFFKGQRILLEGNPARGVYFLLQGKVKLFKTDVRGQEVIIRFAKPGDIVGLSV
                     NTERPENEVSATAMEDTMLCHVNHDAFVELLNSDAKLAFELLRFYRAELHKTEMRSLK
                     LATMTVQEKVADALVTIYEAYGAEGKQRTLCLELSRQDIGNLAGTTKEQVSKVLTDLK
                     NKGMIDTNGKQIDLINLSALMQLAGLA"
     gene            1346..1825
                     /locus_tag="P5292_05935"
     CDS             1346..1825
                     /locus_tag="P5292_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38702.1"
                     /translation="MNQTHIHLLLNHVAILGAIFSFILLLAGSVFGQVVLRKSALFGL
                     VFSAIVAVPVFLTGEPAEEAVEHLPGVTESAIHEHEEAGEAAIWVIGVTGIVSAITLF
                     LGIGPAGKGRRLLVLVYLLAVMSSVSLARTGWLGGKIRHSEISSAAPANVAGEQDDD"
     gene            1899..2087
                     /locus_tag="P5292_05940"
     CDS             1899..2087
                     /locus_tag="P5292_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38703.1"
                     /translation="MLVSSCSTGTNEQTGNQPADTVQSAAAPATEAAVATDSVNAATE
                     KAGQAGEQEEEKEKDDKD"
     gene            2193..2642
                     /locus_tag="P5292_05945"
     CDS             2193..2642
                     /locus_tag="P5292_05945"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRS38704.1"
                     /translation="MFVRLTRKFPFEMAHALYGYDGPCKNIHGHSYRLEVTVLGKALQ
                     ESGHPKDGMLMDFGDLKKIVNEAVVNQFDHALVLNGQSPHKDLDRMRDNFEKIIYVDY
                     QPTCENLLLDFRERIAAKLPNSVRMVSMRLHETANAFAEWNETDQPR"
     gene            2645..>3291
                     /locus_tag="P5292_05950"
     CDS             2645..>3291
                     /locus_tag="P5292_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS38705.1"
                     /translation="MDHRLYVKYNVPGPRYTSYPTVPYWEGAMEQDQWAEELSLQAHA
                     HAAEGISLYVHLPYCESLCTYCGCNTRITVDHRVEGPYIDAVLQEWSMYRKRIGDRPL
                     IKEIHLGGGTPTFFDPAQLRRLILGLLDGCAIADDHEFGFEGHPANTTREHLQTLYDL
                     GFRRVSYGIQDFDPKVQDIIHRFQSVEQVEAVTSWSREIGYTSINYDLVYGLPYQT"
BASE COUNT          807 a          788 c          901 g          795 t
ORIGIN      
        1 aaagtcgtat ccaatgggaa tggctatgtc aagttcccga tcaacgaacc ggcgaccggt
       61 aggaagaaat cgcaggtgga agagtacctc gatttctacg aaggtgccgg ttgccagcat
      121 atcgcggtcg ctacggatga catcatcttc accgtatccg aattgcgcaa gcgtggggtg
      181 gagttcctct atgtgccggg ctcttattac gataccgttc cggaacgggt gggaaaaatc
      241 aaggaagatc tcgaggtgtt gaaaggcttg ggtatcatgg ccgatcggga tgaagagggt
      301 tatttgctcc agattttcac caaaccggtt gaggatcgcc cgacgctttt cttcgagatc
      361 atccaacgtg taggggcgaa atcttttggt aaaggtaatt tccaggcact cttcgaatcg
      421 atcgaagcgg agcaggcaag gagaggaacc ctataacgga taatcagatg ggcggatccg
      481 gttactttca ctgccgccca ttttattgta tattgggaca tgcaaccctc tgccgcgaat
      541 aagaaacagc ttgtagaacc tttctacgaa ctcagttgtc ataactgtcc ggtactggac
      601 tgttcggtct tgcgccattg tacgcaggat tgtcttgaga agatcgataa actcaagaaa
      661 gtagtcggat tcttcaaggg acaacggatc ttattggaag gtaacccggc ccggggcgtt
      721 tatttcctgc tgcaaggtaa ggtgaaattg ttcaagacgg atgttcgtgg tcaggaagtg
      781 atcattcgct tcgctaaacc aggtgatatc gtcgggctga gtgtcaatac cgagcgaccc
      841 gagaatgaag tgtccgctac ggcgatggag gacaccatgc tgtgtcatgt caaccacgat
      901 gcctttgttg aattactgaa ttccgatgca aagcttgctt ttgagttgtt gcgattttac
      961 cgtgccgaac tgcacaagac cgaaatgcgc agcctcaaac tggcaaccat gacggtacag
     1021 gaaaaggtgg ctgatgcact cgtgaccatt tatgaagctt acggagcgga agggaagcaa
     1081 cgcacgctct gcctcgaact ttcccgacag gatattggta accttgcggg gacaaccaag
     1141 gaacaggtca gcaaagtcct gacggacttg aagaacaaag gtatgatcga cacgaacgga
     1201 aaacagatcg acctgatcaa cctgagcgcc ctgatgcaat tggcaggctt ggcttgaacg
     1261 gattaacttt ctgttaaccc attcgggtga cattgctcat gagtgatcgg tcagtcgttg
     1321 ggatactttt gaacaaacat cagccatgaa tcagacccac atccatttac tcctcaatca
     1381 cgttgccatt ctcggagcaa tcttctcttt cattctgctg ttagcgggat ctgtttttgg
     1441 tcaggttgtt ctgcggaagt cggcactgtt cggactggta ttctcggcga tcgtagccgt
     1501 tcccgttttt ctaacgggag aacctgctga ggaagcggta gaacacctgc cgggcgtaac
     1561 ggagtcggcc attcatgagc acgaggaggc gggcgaagcc gctatctggg tgatcggcgt
     1621 aaccggaatt gtctcggcga taaccttgtt tttgggcatc ggcccggccg gtaaaggtcg
     1681 tcgcctcctg gtgctggttt atttgttagc cgtcatgtcg tccgtttcat tggccagaac
     1741 tggctggttg ggcggtaaga tccgtcactc cgaaatcagt tccgccgccc cggcaaatgt
     1801 tgccggtgaa caggatgatg attaaatatc gcatgaagca attcaatatt atagccatga
     1861 aaagactatg gatccccatc gtaacgtgct gtttggcaat gttggtttcg tcctgttcga
     1921 ccggtaccaa cgagcaaacc ggaaatcaac cggctgatac tgttcaatcc gccgctgctc
     1981 cggccacgga agcggctgtt gccaccgact ccgtaaatgc tgccaccgaa aaagccggac
     2041 aggctggtga gcaggaggag gagaaggaga aagacgacaa ggactgatcg tcagatcagt
     2101 gtaatgttct gtgagtttag tggtgcaatg cgccggaaag atgacgctca tcatctttcc
     2161 ggcgtgttct tttattctaa tttgcaagca atatgttcgt acgtctgacc cgaaaattcc
     2221 ccttcgaaat ggcgcatgcc ctgtatgggt atgatggtcc atgtaagaat attcacggcc
     2281 attcctatcg cctggaagtg acggtgctgg ggaaggcgct gcaggaatca ggacatccga
     2341 aagacggaat gttgatggat tttggagacc tgaaaaaaat cgtcaacgaa gccgtagtca
     2401 accagttcga tcacgccctg gtgttgaacg gacaatctcc gcacaaggac ctggatagga
     2461 tgagggacaa tttcgaaaag atcatctatg tcgattatca accgacctgc gagaaccttt
     2521 tattggattt tcgcgagcgg atcgccgcga agttgccgaa ctcggtccgt atggtaagca
     2581 tgcgattgca cgaaactgcg aacgccttcg cggaatggaa cgaaacggat cagccccggt
     2641 aatcatggac catcgtttat atgtgaagta caatgttccg ggtccacgct atacgagcta
     2701 cccaactgtt ccgtattggg agggcgcgat ggaacaagac caatgggccg aggagttgag
     2761 tcttcaggca catgcgcacg cggcagaagg catcagtctt tatgttcacc ttccttattg
     2821 cgaaagcctc tgtacctatt gcggttgtaa tacgcgtatc accgtcgatc atcgggtgga
     2881 aggtccatat atcgacgcgg tcttgcagga atggtcgatg tacagaaagc ggatcggcga
     2941 tcggccgttg atcaaagaga ttcatctagg aggcgggacg cctaccttct tcgatccggc
     3001 tcaactgcgg cgtcttattc tcgggttgct cgatggctgt gccatagccg acgatcatga
     3061 gtttggattc gaggggcacc cggccaatac cacccgggag cacttgcaga ctctctatga
     3121 cctcggcttc cggcgcgtga gttacggcat ccaggacttc gaccccaaag tccaggatat
     3181 catccaccgt tttcaatcgg tcgaacaagt agaagccgtg accagctggt cgcgcgagat
     3241 cggttatacg tcgatcaact atgatcttgt ttacggccta ccgtatcaaa c
//
LOCUS       DAOTON010000247         3289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22955_length_3289_cov_2.825603, whole genome shotgun sequence.
ACCESSION   DAOTON010000247 DAOTON010000000
VERSION     DAOTON010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22955_length_3289_cov_2.825603"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3289)
                     /locus_tag="P5292_05955"
     CDS             complement(<1..>3289)
                     /locus_tag="P5292_05955"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS38706.1"
                     /translation="VTAYAPAGVYCNDSAFKAVTVLDMIAPAAISGPDTVCPGVSYLY
                     NTVPNMNGVTYNWTVTNGTIIGPANGNSVMIQWNAGGGSVNVSQTLTNPPGCTSPASA
                     TFSVYVWPPFSLPTITPSVPIACVKSTIVYSIPPSLISNGTYTWSVVPSTAGNILSAN
                     GTNQITIRWIDASNGPIFVKLKIERCYADSVMFPVNLLGLPPVPNIVAPATVCKNSLV
                     NFSTSSPGPVWNWNFGDAGTANTQNASHTYVTAGNFNVFLAVTNANGCSDTAFTTIQV
                     DDIPQLPVITGPASVCSGSTVTYTFTQPVFPGANYVWTVTAPGVLQAGQGTNTITVKW
                     NSPGSGTVSLAVQSSCPPGPVTPMNVVVNALPTASIGIPSPLCIGAPLTFTGAGGVTY
                     NWSFPGAGNVNLTNQSVPVVTYNATGNFGSSLSIIDANGCTANTNVTFTINPLPLVNV
                     TANEICNFPASVTFNTINTGGYTYLWSTGATTASITQTLTGPTTYSVTVTNAFGCTNT
                     GSKTINGGVCNSIPGVCVVNDSIDFTSVPPLCLTTTFTKIYSGNFTGWDFGDGSNAGP
                     ISPITHTFPTPGVYNVTVQGFAIGMDAMGQPCTTSIARTHPVTIPFDAAFNVTYQCNG
                     LGQMQTIINNTSLYLNSAASYSWNWYLDGNGTPFSSVHSPANQLFSAGPHNVTLIISD
                     PGGTLATCTLTVAVNVPVPVTAAANPPATPPFCAGTPVNFSDASFAAPTSWTWDFYNN
                     GISLSNLQNPQFTYPAAGSFTYKLTVGDQYGCSSSTTRPITINAAGTGTITINNTFCD
                     SIQLTASGVGPFTWSNVVSQPVPDNPFYVKQNGYYSVTGYNPSFGCPYKATVGPLTVK
                     KSPNASISGKTQYCQGENLDLKTSTAGVTVSWNLYPSGPVLGTNPNLTIPANTAGTFT
                     YQVTLTAANGCTATDTISVTVDPVPSSAVIVASGPLTFCEGDSVKLQVVPAAGSYLWS
                     KSPTPPLTPPSNTNSFIWVTQSGTYSVIAQTVNGCSYPNIAPVTVTVNPLPAITITGD
                     TIVCSGKGLSLLATQIAGATYSWVGPNFVSGANPMYQPNMQLSDAGVYTVTITSPNGC
                     TTTRSVNV"
BASE COUNT          733 a          885 c          964 g          707 t
ORIGIN      
        1 acattcaccg atcgtgtggt cgtacaaccg ttcgggctgg tgatggtcac cgtgtacact
       61 cccgcatccg acaattgcat gttcggttga tacatcgggt tggcaccgct gacgaaattc
      121 ggacctaccc aactgtaggt ggcacctgct atctgggtgg ccagtaagct caggccctta
      181 ccgctgcaaa cgatggtatc gccggtgatc gtgatcgccg gcaagggatt gaccgtcacc
      241 gttaccggag caatgttggg ataggaacat ccgttcaccg tctgagcgat aacggaatag
      301 gtgccactct gcgtaaccca gatgaacgaa ttggtattcg atggaggcgt caaaggcggc
      361 gtgggcgact ttgaccagag ataactgccg gcggcaggaa caacctgcaa tttcaccgag
      421 tcgccttcgc agaaggtcaa cggacctgat gccacgatca ccgcggacga tggaaccgga
      481 tctaccgtta ctgagatcgt atccgtagcc gtgcaaccgt tcgctgctgt cagcgtaacc
      541 tgataagtga aggtgcccgc agtgttcgcg ggaatcgtca ggttgggatt ggtaccgagc
      601 accggtcccg acggatacag gttccacgaa acagtcacac ccgccgtgct ggtcttcaga
      661 tcaaggtttt caccctgaca atactgcgtt ttaccggaga tggaggcgtt cggcgatttc
      721 tttaccgtca gcggaccgac cgtggctttg tacggacaac cgaaagaagg attgtaaccg
      781 gtaaccgaat agtagccgtt ttgctttacg tagaatggat tatccggtac cggttgactg
      841 acaacgttcg accaggtgaa cggtcctact ccggatgcgg tgagttgaat ggaatcacag
      901 aaggtgttgt tgatcgtgat cgtacccgta cccgccgcgt tgatggtgat cggtctggtc
      961 gtcgaactgc tacaaccgta ttggtcaccg accgtcaact tgtacgtgaa gcttcccgct
     1021 gccgggtagg tgaattgcgg attctgcaag ttggacaagc tgataccgtt gttgtaaaaa
     1081 tcccaggtcc aggaagtagg cgctgcaaac gaagcgtcag agaagttcac cggcgtaccc
     1141 gcacagaacg gaggtgttgc cggcggattc gcggccgccg tgacgggtac gggcacattg
     1201 accgcaacgg tcagcgtaca agtggccagg gttccgcccg gatcgctgat gatcagggtg
     1261 acgttgtgcg gaccggcgga gaacaactga ttcgccggag agtggaccga actgaacggc
     1321 gttccgtttc cgtcgaggta ccagttccaa ctataggatg ccgcgctgtt cagatacaac
     1381 gaagtattgt tgatgatcgt ctgcatctgc cccaggccgt tacactggta ggtcacattg
     1441 aaggcagcgt cgaatggaat cgtaaccgga tgcgttcgtg ctatggacgt ggtacacggt
     1501 tgccccatcg catccatacc aatggcgaat ccttgcacgg tcacattgta aacaccgggc
     1561 gtcgggaagg tatgggtgat cggactgatc ggtccggcat tgctgccgtc tccgaaatcc
     1621 cagccggtga aattgccact gtagatcttc gtgaatgtcg tggtaagaca caaaggcggc
     1681 accgaagtga aatcaatact gtcgttcacg acgcatacgc ccggaatcga attgcagaca
     1741 ccgccgttga tggttttcga tccggtgttg gtacacccga acgcgttggt gacggtaacg
     1801 gaataggtcg tcggacccgt cagggtttgc gtgatcgacg cggtcgtagc gccggtgctc
     1861 cagagatagg tataaccacc ggtattgatc gtattgaagg tgacactggc agggaaatta
     1921 cagatctcat tcgccgtcac gttcaccagc ggtaatggat tgatcgtaaa cgtcacgttc
     1981 gtattggccg tacaaccgtt cgcgtcaatg atcgaaagac tcgagccgaa attgccggta
     2041 gcattgtagg tcaccaccgg cactgactgg ttggtgaggt tcacatttcc cgcacccggg
     2101 aaactccagt tgtaggtcac cccgcccgct cccgtaaagg tcaagggcgc accgatacac
     2161 agaggggatg gaataccgat cgaggcggta ggcagcgcgt tgaccactac attcattgga
     2221 gtaactggtc ccggcggaca actcgactga accgccagcg acacggttcc ggagcccggc
     2281 gaattccatt tcaccgtgat cgtgttggtc ccctggcctg cctgtagcac ccccggtgcc
     2341 gtgacagtcc agacgtaatt ggcgccagga aataccggct gcgtgaacgt ataagtaacc
     2401 gtactgcccg aacaaaccga agccggaccg gtaatcaccg gcaactgcgg tatgtcatcg
     2461 acttggatgg tcgtgaaggc cgtgtctgaa catccgttcg cgttcgtcac ggcaaggaat
     2521 acattgaaat tacctgcggt tacatacgta tgcgaggcat tctgcgtatt ggcggttccc
     2581 gcgtcaccga agttccaatt ccaaaccggt cccggactcg aagtggagaa gttcaccagg
     2641 ctgttcttgc agacggtagc gggcgccacg atgttcggca ccggcggcaa gcccagcaaa
     2701 ttcaccggga acatcaccga gtcggcatag caccgctcga ttttcaactt cacgaagatc
     2761 ggtccgttcg atgcgtcgat ccatcggatc gtgatctggt tcgtcccgtt cgccgacaag
     2821 atattcccgg cagtagaggg aaccacggac caggtatacg taccgttgct gatcagggag
     2881 ggcggtatcg aataaacgat cgtgctcttc acacaggcga tcggtacact cggtgtgatg
     2941 gtcggcagac tgaagggcgg ccagacataa acgctgaacg tggccgatgc aggactggta
     3001 catcccggag gattggtcag cgtctgcgat acgtttacac taccgcctcc cgcgttccac
     3061 tggatcatca cgctgttccc gttcgcgggg ccgatgatcg ttccgttcgt cacggtccag
     3121 ttgtacgtga caccgttcat attcggaacg gtgttgtaca gatacgagac acccggacaa
     3181 acggtatccg gcccgctgat cgcagccggt gcgatcatgt cgagtacggt cacggccttg
     3241 aaggcggagt cgttacagta gactcccgcc ggcgcatagg ccgtcacgt
//
LOCUS       DAOTON010000248         3288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_22964_length_3288_cov_3.875348, whole genome shotgun sequence.
ACCESSION   DAOTON010000248 DAOTON010000000
VERSION     DAOTON010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22964_length_3288_cov_3.875348"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..816)
                     /locus_tag="P5292_05960"
     CDS             complement(157..816)
                     /locus_tag="P5292_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HRS38707.1"
                     /translation="MNMKIILTGSTGMVGEGVLEECLHNPLVEQVLLINRKTAGVSHP
                     KVREVLHPDFYDITPLVDQLKGYDACLFCMGVSSLGMKEDDYYKVTYTLTLSFAKTLA
                     AFNPNMTFCYISGSGTYSSEKGRLMWARVKGKTENDLMKLPFKAVYNFRPAMLKPSKG
                     ARNVLPAYKYYGFLYPFLRPLSPGFFLTLRELGCAMIRAAANGYPKNILETRDILTLS
                     K"
     gene            complement(955..1185)
                     /locus_tag="P5292_05965"
     CDS             complement(955..1185)
                     /locus_tag="P5292_05965"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRS38708.1"
                     /translation="MIVSYRIDQVSFDQQFLYLQVNGNSFKVKLDQASPKLKAANEMQ
                     RNFFKVSPSGYGIHWPLIDEDLSIEFLMKAAE"
     gene            complement(1189..1437)
                     /locus_tag="P5292_05970"
     CDS             complement(1189..1437)
                     /locus_tag="P5292_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011377918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRS38709.1"
                     /translation="MPTILFIHGWRLFFYSNEGGEPIHVHAEKGDMECKYWLMVDEVE
                     IREAFGFNLNPASRREIKMIIYQHFDLIVESWHQYFKK"
     gene            complement(1570..2238)
                     /locus_tag="P5292_05975"
     CDS             complement(1570..2238)
                     /locus_tag="P5292_05975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRS38710.1"
                     /translation="MTDNQHSGNRGNTPLRILVFSGSMRADSLNTKLALLAKAAIEKH
                     GGQVDFASMREFDCPSYDGDLEAKSGIPAGAQEFQRRLLANDAFILACPEYNGSMPGL
                     VKNTIDWTSRFRPQPFNEKHGMLLSASPSMMGGNRSLWSLRVPFEHLGARIFPDMFSL
                     ATAHQALDSDGRIASATLQQRFDATIAAFLDLVEAARNYPCMKKAWVEFLGQQPDPLR
                     DRLE"
     gene            complement(2531..>3288)
                     /locus_tag="P5292_05980"
     CDS             complement(2531..>3288)
                     /locus_tag="P5292_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRS38711.1"
                     /translation="NGQGDYSPLKNVIDIMPMETDTIEFNASEGYGDWYFHCHILYHM
                     MAGMGRIFSYDQSPPNPQIPDPVRALRKVYADDRRFYLGAEVGLETNGSDGEVQYMNT
                     RWRFSTEWRLGFNRESGRETESHFGRFIDNKQFLFAYAGWDWRDRETHAERNVFDRVS
                     TKNDRSVVHAGLQYVLPWFIVVDLSLDHTGYFRAQVRRDDLALTRKIRLWGFWNTDRE
                     YSLGARYIVDRLWSLSAHYDSDMGPGMGLTFTY"
BASE COUNT          784 a          868 c          877 g          759 t
ORIGIN      
        1 actcttcggt gagcggactg tccagggcga tcttgcggag caggctcagc tccttttccc
       61 agggaccttt ccgggcaaac tgctcgtcga ctttcggatg ggtgttttgc tttgccattt
      121 gcagatgatg gttattggga agtcagtcga atgaaatcac tttgacaagg tcaggatatc
      181 gcgtgtctcc aggatgtttt tcggatatcc gttcgcggcg gcgcggatca tcgcacaacc
      241 cagttcccgc agcgtgagga aaaaaccggg cgacaacgga cgcaggaacg gatacagaaa
      301 accgtaatac ttatacgccg ggagcacgtt acgcgcacct ttcgacggct tgagcatggc
      361 tggcctgaaa ttgtacaccg ccttgaacgg taacttcatc aggtcgtttt ccgtcttgcc
      421 cttcacccgt gcccacatga gccgtccttt ttccgaactg taggttcccg agcccgagat
      481 gtaacagaag gtcatgttcg ggttgaacgc ggcaagcgtt tttgcgaacg aaagtgtaag
      541 cgtataggtg accttgtagt agtcgtcctc cttcatgcca agcgaagaaa cgcccataca
      601 gaacagacag gcatcgtagc ctttcagctg atcgaccaaa ggcgtgatgt cgtaaaagtc
      661 agggtgaagg acctcgcgca ccttcgggtg cgagacccca gccgttttac ggttgatcaa
      721 caggacttgc tccacgagcg gattatgcag gcattcttcc agaacgcctt cgcccaccat
      781 gccggtggaa ccggtgagga tgattttcat attgatatca cctgcatgcg aatccgggaa
      841 tcgggttcac atcttagagg gacaaccaga taatccctat caataaactc gaacactgca
      901 caaaatccga tccttcagca ggacaacagt catgatccaa actgaaaacc gtctttattc
      961 tgctgccttc attagaaatt ctattgacag atcttcatcg atcagaggcc aatgtattcc
     1021 ataaccggaa ggcgatacct taaaaaaatt tctttgcatc tcattagctg ccttgagttt
     1081 cggagaagcc tgatcaagct tgaccttgaa ggaatttcca ttcacctgca agtagaggaa
     1141 ctgctgatcg aacgaaactt gatcaattct ataggacacg atcatgcttt actttttgaa
     1201 gtactgatgc caggattcaa cgattaagtc gaaatgctga tagataatca tcttgatttc
     1261 tcttcttgag gccggattga ggttgaatcc gaaagcttcc cgaatctcga cctcatccac
     1321 catgagccaa tacttacatt ccatatctcc cttttcagca tgaacatgaa taggttcgcc
     1381 tccttcattg gaataaaaaa agagtcgcca gccatggatg aacagaattg taggcatcgg
     1441 tattctcaaa gttacgaaaa cccatgaatt gtacttgcta atgatcttat cagggacaga
     1501 atatccaatg ttacattttc atcagaaacg ccattcccga ttttgccagc aaggaaaatg
     1561 aaggccatgc tactctaacc tgtcccgcaa cggatccggt tgctgtccta ggaactccac
     1621 ccaggctttc ttcatgcacg gatagttgcg ggcggcctcg acgaggtcca ggaaggcggc
     1681 gatggtcgcg tcgaagcgct gttgcagcgt agcgcttgcg attcggccat ccgaatcgag
     1741 cgcctggtgc gcggtggcca gcgagaacat atcggggaag atgcgggcgc cgaggtgttc
     1801 gaacggaacg cgcaacgacc agagggaacg gttgccgccc atcatcgaag gggaagccga
     1861 caacaacatg ccgtgctttt cgttgaacgg ctgtgggcga aagcgactgg tccagtcgat
     1921 ggtattcttg accaggccgg gcatgctgcc gttatactcc ggacaagcca ggatgaacgc
     1981 gtcgttcgcc agtaaccggc gttggaactc ctgcgcaccg gcggggatgc cgcttttcgc
     2041 ctcgaggtcg ccgtcgtacg acggacaatc gaactcgcgc atcgatgcga aatcgacttg
     2101 gccaccatgc ttctcgatcg ccgctttggc gagcaacgcg agtttggtat tcagtgaatc
     2161 ggcccgcatc gagccggaga agacgaggat gcgcagcgga gtgttgccgc ggttgccgga
     2221 gtgttggttg tcagtcatgg agggataaat taagatgcaa gagtttcgtt gaccatcgga
     2281 tctgctatgg gaagaccgta ttgagtctta tggaaatctg aaaattaccg aaaaagttgt
     2341 atcgactgac tacatcggtc ggttatggct tagtcccttt ctgtgaacca ttcgagccgc
     2401 cgtcggtgca ccgccacaac acaatggcgg aacgggcttg tcagaacgga ctccgtgacg
     2461 gcagctgata ggcctggatg cgggtgatga ggccgagtag ggcagagagc ccttggcctc
     2521 tgataaatta ttagtatgta aaggtcaggc ccatgccagg acccatgtcg ctgtcatagt
     2581 gcgcggaaag cgaccagagc cggtctacga tgtaacgtgc gccgaggctg tactcccggt
     2641 ccgtgttcca gaatccccac aacctgatct tgcgcgtcag ggccaggtca tcgcgtctga
     2701 cctgcgcgcg gaaatagccg gtatggtcga gtgaaagatc caccacgatg aaccagggca
     2761 ggacgtactg caatccggca tgtactacgc ttcggtcgtt cttggtgctg acgcgatcaa
     2821 acacgttccg ctccgcatgg gtctcgcgat cacgccaatc ccaaccggca taggcgaaga
     2881 ggaactgctt gttgtcgatg aaccgtccga aatgactctc ggtctccctg ccagattcgc
     2941 ggttgaagcc caggcgccac tcggtactga aacgccagcg cgtattcatg tactggacct
     3001 ctccgtcgct gccgttcgtt tccaggccta cctccgcgcc aagataaaac cgccgatcgt
     3061 cggcatacac cttccgaagc gcccggacgg ggtcggggat ctgcgggttc ggaggcgatt
     3121 ggtcgtagct gaagatccgg cccataccgg ccatcatgtg ataaaggata tggcaatgaa
     3181 aataccagtc gccatagcct tccgatgcgt tgaactcgat cgtatccgtt tccatcggca
     3241 tgatatcgat cacgtttttc agcggcgaat aatccccctg accgttca
//
LOCUS       DAOTON010000249         3281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_23020_length_3281_cov_3.386237, whole genome shotgun sequence.
ACCESSION   DAOTON010000249 DAOTON010000000
VERSION     DAOTON010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23020_length_3281_cov_3.386237"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="P5292_05985"
     CDS             complement(<1..1204)
                     /locus_tag="P5292_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRS38712.1"
                     /translation="MENFLYVVPVLGVVGLLYMALKAKWVTAQDAGDSKMQGIATAIA
                     EGAMAFLRAEYRILAIYMVIAGTALGILSQIVESSHILIVLSFVIGCIFSALAGFIGM
                     RIATKANVRTTQAARTSLARALNVSFSGGLVMGLGVAGLAVLGLSLLFIFFYQYFMNG
                     QMASYQQMTQVLEVLAGFSVGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPATIADNVGDNVGDVAGMGADLFGSYVATVLASMVLGNYIIRDMGGIEDAFGGIG
                     PILLPLSIAGVGILASILGSFFVRVADNAQANTDTVQSALNKGNWFSILLAVVASFFL
                     IRLMLPETITMSVFNAGEFSEMTTTSMNVFWAVVIGMFVGGAISYITEFYTGLGKKPV
                     LSIVQKSAT"
     gene            complement(1353..3182)
                     /locus_tag="P5292_05990"
     CDS             complement(1353..3182)
                     /locus_tag="P5292_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS38713.1"
                     /translation="MRYLLLFTITWLSLQHLASAQTFNGSGGPIHDLNGTPQAQYFPC
                     VVSGLPNSIDSSTFGLERICLDITHTYTNDLRLQLMSPDSLLLDLCNRHGGGGDNFTG
                     TCFRGMSVNGMISATGNIPPYTGEYDPDQDMALFNNGQDPNGTWFLVVTDMAGADSGT
                     VDTWSLTFGSDPTPHGLEPCNMLRPALCICPDGTQDCDLLPDMTASEQAILDGSYERN
                     DTLFVNNATPNIGWGPLEIHGTGDCYCDTVPVNCSITCPDGSSPKEIVQQTVYHKSFN
                     TMTSYVRQAGTMAYHPQHGHIHLDGWAEYTLRKAWHGKTPQDWPVIGTGNKQSYCLVN
                     LGQCTAGNGYCVDTTGQIRYNANIANYGLGSVTGCSRDQGIFVGSLDIYSASLYGQWI
                     ILDSVCNGDYYIVSITDPNNWILETDDSNNWAAAPFSLTGQLNAPFPTVNFTYSATGS
                     SVQFTSSCADFDSLSWDFGDGDTSTALNPLHTYASTGTYEVVLTAFNHCGWEQQVQSF
                     TITVSGLEAIGHEDLFGLRVFPNPSSTAFNIDFSTASRTDVRLELLDIMGRPVSVLSD
                     QSMAAGSHRYRLDVESGNLQAGVYFLRVQSDGVARLVRLVVAR"
BASE COUNT          812 a          854 c          931 g          684 t
ORIGIN      
        1 cggtagcgga tttctggaca atcgaaagga ccggcttctt gccgagtccg gtgtagaatt
       61 cggtgatgta ggaaatggcg ccgccgacga acatgccgat cacgaccgcc cagaagacgt
      121 tcatggacgt ggtagtcatc tcgctgaact ctcctgcgtt gaacacgctc atcgtgatcg
      181 tttcaggaag catgagccgg atcaggaaga aagaagctac tacggccagc aggatggaaa
      241 accagttgcc tttgttcagg gccgactgca cggtatcggt gttggcctgt gcattgtcgg
      301 caacccgtac gaagaaagaa ccgaggatcg atgcgaggat gccgacaccg gcaatggata
      361 acggaaggag gatcggaccg atcccgccga acgcgtcttc gatgccgccc atatcgcgga
      421 tgatgtagtt gcccagcacc atcgacgcaa gcaccgttgc tacatacgaa ccaaagagat
      481 cggctcccat acccgcaacg tcaccgacgt tgtcgccaac gttatcagcg atcgtggccg
      541 gattgcgggg atcgtcttcg ggaatacccg cttccacttt gccgacgagg tctgcgccaa
      601 cgtcagcggc tttggtatag attccaccac caacgcgagc gaacagcgcg atcgattccg
      661 caccgaccga gaatccggct agtacttcga gcacctgggt catttgctgg tagctggcca
      721 tttggccgtt catgaagtac tgatagaaga agatgaacag caggctcaat ccgagtaccg
      781 caagtccggc gacgccgagt cccatgacaa gtccgccgga gaaggagaca ttcaaggccc
      841 gggccagcga agtgcgggcg gcctgcgtgg tgcgaacgtt ggctttggtc gcgatgcgca
      901 ttccgatgaa accggccaat gcactgaaga tgcagccgat cacgaaggag agtacgatca
      961 ggatgtgcga ggactcaacg atttgagaca gaattcccaa cgcggtaccg gcaatcacca
     1021 tatagatggc caggatccgg tactctgcac gcaaaaaggc catggcgcct tcggcgatgg
     1081 cggtggcgat gccctgcatc ttggaatcac cggcatcttg cgcggttacc cacttggctt
     1141 tcagggccat atacagaaga ccgacgacac ccagaaccgg tacgacgtac aagaaatttt
     1201 ccattttaac gcttttttga agggctgcaa aggtaagcca tcagagcaaa aaaccaaatt
     1261 gcccttgaag gtaaaaggcc ctgaaaaaca gaaacttgca aggtccccgc aggagccctt
     1321 gcaagtttcg caggatgatt accagccttg aattaacggg cgacaaccag ccgcaccaac
     1381 cgtgctacac cgtctgattg aacccgcagg aaatagacac ccgcttgtag gttgccggat
     1441 tcaacatcca gccgataccg gtgactgccg gcggccatgg attggtcact caatacgctc
     1501 acaggtcgtc ccatgatgtc gagtaactcc aggcgaacat cggtgcggct tgccgtgctg
     1561 aaatcgatat tgaatgcggt ggacgaagga ttcggaaata cacgaagacc gaacagatct
     1621 tcatgaccga ttgcctccag gccagaaaca gtgatcgtga acgattgcac ctgctgttcc
     1681 cacccacagt gattgaaggc ggtcaatacc acctcgtagg tgccggtgga tgcataggtg
     1741 tgcaaggggt tcagcgcggt gctggtatcg ccatcgccga aatcccagga gagtgaatcg
     1801 aaatcggcac agctggatgt gaactgtacc gatgaacccg tagcactgta cgtgaaattg
     1861 acggtcggga atggggcatt gagctggccg gtgagcgaaa atggcgctgc ggcccaattg
     1921 ttactgtcgt cggtctcgag gatccagttg ttggggtcgg tgatcgaaac gatataataa
     1981 tcgccgttgc agacgctgtc caaaatgatc cactgaccat aaagcgaagc cgagtaaata
     2041 tccaatgagc cgacgaagat gccctgatcg cggctgcatc ccgtcactga tccaagaccg
     2101 taattcgcga tgttggcatt gtagcggatc tgaccggtgg tatcaacgca ataaccattt
     2161 ccggctgtac actgtccgag gtttaccagg caataacttt gcttgttacc ggtgccgatg
     2221 acgggccaat cctgcggagt cttgccgtgc caggctttcc gaagggtgta ttccgcccag
     2281 ccgtcgaggt gaatgtgacc gtgttgagga tgataggcca tcgtacctgc ttggcgaaca
     2341 taggaggtca tcgtattgaa cgatttgtga taaacggtct gctgtacgat ttcctttggc
     2401 gagcttccgt cgggacaggt gatgctgcaa ttcacgggaa cggtatcgca ataacaatca
     2461 ccggtgccgt gaatctcgag tggaccccag ccgatgttgg gcgtggcatt gttgacgaac
     2521 agggtgtcgt tgcgttcata ggatccatcc aaaatggctt gttcggatgc ggtcatgtcc
     2581 ggcagcagat cgcaatcctg agtgccatcc gggcaaatgc agagtgccgg tcgcagcatg
     2641 ttacaaggtt ccagaccgtg tggagtaggg tcgctgccaa aggtcagcga ccaggtatcc
     2701 accgtacccg aatcggctcc ggccatatcc gttacgacca ggaaccaggt gccgttagga
     2761 tcctgcccgt tgttgaacaa ggccatatct tggtccggat catattctcc ggtataggga
     2821 ggaatattgc cggtggcgga gatcataccg ttcacactca tcccccgaaa acaagtcccg
     2881 gtgaaattgt caccgccacc gccatgccgg ttgcagagat ccaacagcaa actatcggga
     2941 ctcatcaatt gcagtcgcaa gtcattggta tacgtatggg taatgtcgag gcaaatgcgt
     3001 tcgagtccga aggttgaact gtcgatactg ttcggtaggc cactcacgac acacggaaaa
     3061 tactgggcct gcggtgttcc attcagatcg tggatcgggc caccacttcc gttgaaagtt
     3121 tgtgcagagg caaggtgctg cagcgacaac caggtgatgg tgaagagtag tagatagcgc
     3181 atgatttcct ttttggcgtt gacaaattaa caggaaatcc gcagaaatga aacaggcgat
     3241 cccaatggat cgcctgtttt gatcaaacaa atgacgagac t
//
LOCUS       DAOTON010000250         3280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_23025_length_3280_cov_3.288372, whole genome shotgun sequence.
ACCESSION   DAOTON010000250 DAOTON010000000
VERSION     DAOTON010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23025_length_3280_cov_3.288372"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2778)
                     /locus_tag="P5292_05995"
     CDS             complement(<1..2778)
                     /locus_tag="P5292_05995"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38714.1"
                     /translation="MKTLLFVATIFCCAITSRAQTSWEKLFSRTSTDAFRNVAECPSG
                     GYIAVGYTSSWSPNDTDAYVVRLNTSGDTIWTRRINGTGSRKDLLYKVINTNDGNYAM
                     CGYSTSYGAGTEDVLIIKMDDNGNILWTKTWGGTGRDRAQEIVQTSDNGYIVCGYTTS
                     PPAQYFDGFLLKLNASGDTTWSRRYGTALFEDFNSVKQLADGGYIAGGQGNNGGNGLD
                     QYLVRTNSSGDTTWTKRFGTLGTDNIEYIHPVADGFLLAGGTDGTGSDGNNGYIVKTD
                     LSGVVVWTKVYGGSLADDFHEIEPTLDGNFILSGTTSSSGPIDPNMWMFKVDANGDSI
                     WARTYGGNNHDHGYSAMPTSDGGYIFAGYSSSFGFNYEEAYLVKTDANGLLSNFLTYI
                     TIFSLASPLEGVCGNATQQVKVVLRNFGRDTVPNIPITVNITGAITQTLNFNYTGPLF
                     PEDADTVTFLSTINTSGGGAFNFGCVTGTTNDVYPANNRFDTTRTIIAFSTAPTTTGA
                     ARCGPGSVTLSASATDPILWYSASTGGALLGSGNTYNTPSISSTTTYYAQAGSTCPSN
                     RVAAVATINTAVADPVTTGADRCGSGSLTLTATASDPVTWWDAASGGTQVGTGNSFVT
                     PNLTTTTTYYAQASNGPCSSARIPTVATINTQPNTPTTTGAFNCGPGTVTLGASSSAS
                     LVTWWDAASGGNQVATGLSYTTPVLSSTTTYYVEASNGTCPSSARASVVATIRSITPD
                     PGTSGGARCGSGTVTLNATSAQTLIWYSSPGGAQVGVGASFTTPFLTTSTTYYVQATD
                     GFCPSQYVAVQAVINTPPSVFLGNDTTVLATSYQLNAGSGFTGYNWNSGAGTAQTFTV
                     TTPGTYCVVVTDGNGCTATDCIFVDLITGINEQSDWFATVYPNPAHEQLTIRFGGVQP
                     KTLSFRILHMNG"
     gene            complement(2832..>3280)
                     /locus_tag="P5292_06000"
     CDS             complement(2832..>3280)
                     /locus_tag="P5292_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38715.1"
                     /translation="ADHSGQTVTLDAGAVFSSYLWSTTATTQTINTGVSGVYSVTVTD
                     GNGCTGSDSIVVNIITGLNAISGDIQLNIFPNPSNGRVEVTAVGVSESFELRITDQLG
                     RVIFFEPVRQTNIYRKSIDLSEKAQGVYFLQLSTSKGRVTRALIIE"
BASE COUNT          657 a          870 c         1017 g          736 t
ORIGIN      
        1 tccattcatg tgcaggatgc ggaacgacag cgttttcggt tgaacgccac cgaaccggat
       61 cgtcagttgt tcatgcgcgg ggttcgggta aaccgtcgcg aaccagtcgg attgttcgtt
      121 gataccggtg atcaggtcga cgaaaatgca atccgtcgcc gtgcatccgt taccatccgt
      181 cacgaccacg caataggtgc cgggtgtggt gacggtgaat gtctgagcgg tgccggctcc
      241 gctgttccag ttatagccgg tgaagcccga tcccgcgttg agctggtagg aggttgccaa
      301 cacggtcgtg tcgtttccga ggaacaccga cggaggcgta ttgatgacgg cttgtaccgc
      361 cacatactgg ctcggacaga acccatcggt agcctgcaca tagtaggtgg tgctggtcgt
      421 caggaacggc gtggtgaacg aagcgccgac tcccacctga gcgccgcccg ggctactgta
      481 ccagatcagt gtttgcgcgc tggtcgcgtt gagcgtaaca gtgccgcttc cgcagcgtgc
      541 gcctccactt gtgccggggt caggggtgat ggaccgaatg gtcgcgacca ccgacgcgcg
      601 cgccgatgaa ggacaagtcc cgttggaagc ttctacataa taggtggtgg tgctgctgag
      661 caccggtgtc gtgtaactca agccggttgc tacctggttg ccgccgctcg ccgcatccca
      721 ccaggtcacc agtgaagcgg aagatgaagc gccaagtgta acggtgccgg gtccgcagtt
      781 gaaagcgccg gttgtagtcg gtgtattggg ttgggtattg atggtcgcca cggtaggaat
      841 ccgtgcactg gagcaagggc cgttggaagc ctgggcatag tacgtcgtgg tagtcgtcag
      901 attcggcgta acgaaactat tgcccgttcc gacttgtgta ccgccggaag cggcgtccca
      961 ccaggtaacc ggatcgcttg cggtcgcggt cagcgtcaac gaacccgatc cgcaacgatc
     1021 cgcaccggtg gtcaccggat cggctacggc cgtatttatc gtcgcgactg ctgctactcg
     1081 attcgacgga caagtgctgc cggcttgagc atagtaggtg gtagtactcg aaatggaagg
     1141 cgtgttatag gtgttgccgg agcccagcaa cgctccgccg gtcgacgcac tgtaccacaa
     1201 gatcggatcg gtagctgaag cggaaagggt cacactgccg ggaccgcatc gcgccgcgcc
     1261 ggtagtcgtc ggggcggtgg agaatgcgat gatggtacgc gtggtgtcga agcggttgtt
     1321 agccgggtaa acgtcgttcg tggtaccggt tacacacccg aaattgaatg caccaccacc
     1381 ggaggtgttg atcgtggaga ggaaggtgac ggtgtccgcg tcttccggaa acagcggtcc
     1441 cgtatagttg aagttcaggg tctgcgtgat ggcgcccgtg atgttgacgg tgatcggaat
     1501 gttcggtacc gtatcgcgac cgaaattccg cagaacgacc ttgacttgct gcgtcgcgtt
     1561 gccgcaaacg ccttcgagtg gtgaagcaag actgaagatc gtgatgtagg tgaggaaatt
     1621 cgaaagcagt ccattcgcgt cggtctttac gagatacgct tcctcgtagt tgaagccgaa
     1681 gcttgaacta taaccggcga agatgtagcc gccatcggag gtcggcatgg cgctgtaccc
     1741 gtggtcgtgg ttattgccgc cgtaggtgcg tgcccagatg gagtcgccgt tcgcgtcgac
     1801 cttgaacatc cacatgttcg gatcgatcgg accggagctg ctggtggtgc cgctgagaat
     1861 gaagttaccg tcgagtgtcg gctcgatctc gtgaaaatcg tcggccagtg aacctccgta
     1921 tactttggtc cagacaacca ctccgctcag atcggtcttg acgatatagc cgttgttgcc
     1981 gtcggagccg gtaccatccg ttccgcctgc cagcaggaat ccgtccgcaa ccgggtggat
     2041 gtattcaatg ttatcagtgc cgagcgttcc gaatcgctta gtccaggtcg tatcaccgga
     2101 agaattggta cgcacgaggt actgatcaag tccgttacca ccgttgttac cctgtccgcc
     2161 ggcgatataa ccaccatcgg caagctgttt caccgagttg aaatcctcga agagtgctgt
     2221 tccatagcga cgcgaccagg ttgtgtcgcc gctggcgttc aacttgagga ggaagccgtc
     2281 gaagtattgc gccggagggg atgtggtata gccgcaaacg atgtatccgt tatcgctcgt
     2341 ctggacgatc tcctgcgcgc ggtctcgacc ggtcccgccc caggtcttgg tccagaggat
     2401 gttcccattg tcgtccatct tgatgatcaa tacgtcttcc gttccggcgc cgtagctcgt
     2461 cgagtagccg cacatggcgt aattgccgtc gttggtgttg atcaccttat agagcaggtc
     2521 cttcctgctg ccggtgccgt tgatgcggcg cgtccaaatt gtgtcgcctg aggtattgag
     2581 ccgaaccacg tacgcgtcgg tatcattcgg agaccaactg gaggtatagc cgacagcgat
     2641 gtagccgcct gacggacatt ccgcaacatt tctgaaggca tccgtgctgg tgcgggagaa
     2701 cagcttttcc caactggtct gtgcacgtga ggtaatggcg caacagaaga tggtcgcgac
     2761 aaacaggagt gttttcaagg atagtttcat acagaggtgg ttaaacgatt tttccgttta
     2821 cgaggattat tttattcgat gatcaaagca cgggtaacac gtcccttcga agtgctgagc
     2881 tgaaggaagt aaacaccctg cgctttttct gacaggtcga tgctcttgcg atagatgttc
     2941 gtctgccgga ccggttcgaa gaagatcaca cgacccagtt ggtcggtgat gcgcaactcg
     3001 aaggattccg atacacccac cgccgtcact tcgacgcgcc cgttcgacgg attcgggaag
     3061 atgttgagct ggatgtcgcc gctgatggca ttcagtcctg tgatgatgtt caccacaatg
     3121 gagtcgctac cggtacagcc gtttccatcg gtaacggtaa ccgaatacac gccgctcaca
     3181 ccggtgttga tggtttgcgt ggttgccgtc gtgctccaga ggtaactact gaataccgcg
     3241 ccggcatcga gggtgacggt ttggccggag tggtcggcac
//
LOCUS       DAOTON010000251         3279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_23041_length_3279_cov_3.374690, whole genome shotgun sequence.
ACCESSION   DAOTON010000251 DAOTON010000000
VERSION     DAOTON010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23041_length_3279_cov_3.374690"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="P5292_06005"
     CDS             <1..689
                     /locus_tag="P5292_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRS38716.1"
                     /translation="LSWGEAPDLDAMVEAYLDRKAGITPSENLYQSRRKRGRDAAILV
                     LTDTSLSTDGYSNNQRVLDLEKEALVLVADVWEKLGVRFQLDTFNSRTHNHCNYGTVK
                     GFHESWQLCRDRIGAIGSSGYTRIGPALRHATYLLNQVRASSRWLLLLTDGKPNDYDR
                     YEGQYGIQDVRQAIREAERDGVHVSAIAIEEAARFYLPQMFGKGGFELLRHPRQLPFA
                     LLNGYLRIVR"
     gene            complement(757..975)
                     /locus_tag="P5292_06010"
     CDS             complement(757..975)
                     /locus_tag="P5292_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38717.1"
                     /translation="MTNESLDEQRLKRAKQQNLGFILAGIGAVLGFISCVLTLWNPIP
                     SIYDLVLYGLTSIAILVIFAGLYLIFEG"
     gene            1160..>3279
                     /locus_tag="P5292_06015"
     CDS             1160..>3279
                     /locus_tag="P5292_06015"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRS38718.1"
                     /translation="MRISTIATFVAALLLLSGYKPLSAQTLPPGFSQVLVAPGITQPT
                     TMAYTPDGRFFICEQTGAVKVLKNDTLLSRPFITLTTNLNGERGLVGIAIDPDFTNNQ
                     YVYLCYTIPTGEFNRVSRFTASGDTVVPGSEVVLLETDTMIANYHGGGHLQIGPDGKL
                     YVAVGENGRSALSQDLNSYLGKILRINLDGSTPSDNPFPGPGKQRNIWAYGLRNPFTF
                     SFQPSTGRFFINDVGEITWEEINDATAGGGNYGWPLAEGVSSDTAFDNPFYSYLHGLS
                     TGQGCAITGGDFFDPDTTNYPTAYQDKYYFIDYCGNWIDYITLSYPPVWSHFASNVAN
                     YSVGLMTGPDGNLYFLSRNNEALYKIAYSPNPAAVVVNEPLDQLVSLGYPANFSVSAS
                     GTPPFQYQWYKGTTLLLNDTNATLNIPNCAFADSGDYRVVVSNAFGSDTSRYAHLSVT
                     ANQPPVAVINTPLPGAHYSGGDLISFSGDANDPEDGVLPDSAFQWVVIFHHDTHVHPG
                     PNAGSGIRSGAFLVPTTGEVSPNVFFRLYLIVRDAEGLVDSAFVDLQPNLSDFTINTQ
                     PQGLTIYVDGQPHIAPHTVTGVEGMYRNFGAPWSQVSGPSTYFFTRWDNGGALTQTFR
                     TPVNDTAWTAFYGEDVLPFTLNDTVVCINSTLQLSPAGYNSYAWSDGTTGNNYTFNSS
                     VPDTFSIGLTVTDGFNADDFVSVA"
BASE COUNT          723 a          933 c          855 g          768 t
ORIGIN      
        1 gcttgtcgtg gggagaggct cctgatcttg acgcgatggt ggaagcttat ctggaccgga
       61 aggcaggcat cactccttcg gaaaatttgt atcagtcgcg ccggaagcgt ggccgggatg
      121 cggcgatcct ggtattgacc gataccagcc taagcaccga tggatattca aacaaccagc
      181 gggtattgga tctggagaag gaggcgctgg tacttgtcgc ggacgtttgg gaaaagttgg
      241 gtgtccgctt ccagcttgac acgttcaata gccggacgca taaccattgc aactacggaa
      301 cagtaaaggg ctttcacgag tcctggcaat tgtgccgcga tcgcattggc gcaattggaa
      361 gttcgggtta tacccgcatc ggcccggcac tacgacacgc gacctatctg ttgaatcagg
      421 tgcgtgcttc cagtcgctgg ttattgttgt tgaccgacgg aaagccgaat gattacgacc
      481 ggtacgaagg tcaatacggt atccaagatg tacggcaggc cattcgggaa gcggaacggg
      541 atggggtgca tgtctcagcg atagccatcg aagaagcagc acggttttat ttgccccaga
      601 tgttcggtaa aggtggcttt gagttgttga ggcatcctcg ccaattgccg ttcgctttgt
      661 tgaatggata cctgcgaatc gtgcgctgaa agccccctga acaattcact gaaaaaatta
      721 gtccctgatc cctttcgtcc ctcgtctcac ccgcgattac ccttcgaaaa tcaggtatag
      781 ccccgcaaag atgaccagta tggcgatgga agtcagaccg tacaggacca gatcatagat
      841 ggagggaatc ggattccaga gtgtgagcac acagctgatg aatcccaaaa cggctccgat
      901 gccggcgagg atgaatccga gattctgttg cttcgcccgc ttgaggcgtt gctcgtctag
      961 ggattcgttg gtcatagtcg aaagataatc attattcgat attgtcctat atcctttcgt
     1021 attgaatggt agaaaagccg ggaagccgtc gttgcgcccc cgtttgccgt actaactatt
     1081 aattctccgt aaacgatcgg gaattcatac tgactctaaa gatttttttc ttttttcggg
     1141 tctctgcgcc atcacccata tgcgaatttc caccatcgcg acctttgtgg ccgccctctt
     1201 gttgctgtcc ggttataagc cgctttcagc acaaaccctt ccgcccggat tctcacaggt
     1261 gctggtcgcg ccgggcatca cccagccgac gaccatggct tatacgcccg atggtcgttt
     1321 ctttatctgt gaacagaccg gagctgtcaa ggtcctgaag aacgatacgc tcttgtcgcg
     1381 tccgttcatc accctcacca ctaacctgaa cggtgaacgc ggcctggtcg gaatcgccat
     1441 cgaccccgac tttacaaaca accaatacgt ctacctctgc tacacgatcc cgaccggtga
     1501 gttcaatcgg gtcagtcgtt tcaccgccag tggcgatacg gtggtgccgg gtagtgaagt
     1561 agtattgctg gaaaccgata ccatgatcgc caactatcat ggcggagggc acctgcagat
     1621 cggtccggac ggcaagctct atgtcgcggt tggtgaaaac ggtcggtccg cactctcgca
     1681 ggacctgaac agctacctcg gaaaaatcct ccgcatcaat cttgacggat caaccccgtc
     1741 cgacaatccg tttcccggac ccgggaagca gcggaacatt tgggcctatg gcctgcgtaa
     1801 tccgttcacc ttttcgttcc agccctctac cggtcgcttc ttcattaatg atgtcggcga
     1861 gataacctgg gaagagatca atgacgcaac agccggaggc ggtaactatg gttggcctct
     1921 ggcagaaggc gtcagcagcg ataccgcctt cgacaatcct ttttactcct acctgcacgg
     1981 gctttcgacc gggcagggct gtgccattac cggaggtgat tttttcgatc cggatacgac
     2041 taattatccg accgcgtacc aggacaagta ttacttcatc gactactgcg gcaactggat
     2101 cgactacatc accctgagtt acccgccggt gtggagtcac ttcgcctcaa acgtcgcgaa
     2161 ctatagcgtc ggcttgatga ccggccccga tggtaacctg tacttcttat cccgcaacaa
     2221 tgaagcgtta tacaagatcg cttactcgcc caatcctgct gccgttgtgg taaacgaacc
     2281 actggatcag ttggtgtcgc ttggatatcc ggctaacttc agtgtctctg ccagtggtac
     2341 acctcctttc cagtaccagt ggtacaaggg aacgaccttg ctgctcaacg atacgaacgc
     2401 cacactcaac atcccgaatt gcgcgtttgc ggactccggc gattaccgcg tcgtggtcag
     2461 caatgccttt ggatccgata ccagccgtta cgcgcacctg agtgttacag ccaaccaacc
     2521 gccggtcgcc gtgatcaata caccgcttcc cggggcacat tacagcggcg gcgacctcat
     2581 cagcttctcg ggtgatgcca atgatccgga agacggcgta ctgcccgact ccgccttcca
     2641 gtgggtcgtc atctttcacc acgacacgca cgtacatcca ggacccaatg ccggaagcgg
     2701 tattcgcagc ggagcttttc tggtgccgac taccggcgag gtttcgccca atgttttttt
     2761 ccggctctat ctgatcgtac gggacgcgga aggcttggtc gattcggcgt tcgtcgacct
     2821 gcaacccaac ctcagtgact ttaccatcaa cacgcaacca caaggattga ccatctatgt
     2881 ggatgggcaa ccccatatcg cgccgcatac ggtgacgggc gtcgagggca tgtaccgcaa
     2941 cttcggcgcg ccttggtcgc aggtctcagg accatccact tatttcttca ctcgctggga
     3001 taatggcggt gcgctcacac aaacgttccg tacgccggtg aacgataccg cctggaccgc
     3061 tttttatggt gaagacgtgc tacccttcac gctcaatgat accgtggttt gcatcaattc
     3121 gaccttacaa ctgagcccgg cggggtataa cagctatgcc tggagcgacg gaacgaccgg
     3181 gaacaactat acgttcaact cgtcggttcc cgacaccttc agcatcggac tgactgtcac
     3241 cgacggattc aatgccgatg acttcgtgtc ggtcgccca
//
LOCUS       DAOTON010000252         3277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_23063_length_3277_cov_3.308194, whole genome shotgun sequence.
ACCESSION   DAOTON010000252 DAOTON010000000
VERSION     DAOTON010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23063_length_3277_cov_3.308194"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="P5292_06020"
     CDS             complement(<1..1528)
                     /locus_tag="P5292_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRS38719.1"
                     /translation="MKIRLNFIFLLCCYTSYIPSAYSQWYTEPRIDTIYQDSTNYLFN
                     SPVSLPSTGNFVFTDTARALVIQSTEYIQLREGFVASAGELCGKYVLGIANCPTLQST
                     ATVTQVSCNGGTNGAVQLFSSGGREPYYFKWDNLGQVGSFMNGRKAGTYSYTIFDTSN
                     CITTGSVQIAEPAELVIDAHRVSPTCNLSNGSISLSCTGGSGPYIYDWIGYDSEDSIL
                     TGIPGGLYDLVVFDAKGCMVDTTLSLSDSMSAQFSTLITPLFLCDDPSTVILNFLSPA
                     QDEAVPVNTCDEMPYRYAAGTHDFVFEDTSGCVSVQTINIPDRVPLTLSFAQEPSASS
                     TDSSLLTLNSDGGYPPYAYQLDGAPISSNQVVAVNGLRMVSVTDSVGCFQQRTIYLGP
                     INVADLGIQVSGPSCYGGQDGEILILDSLFVGNPAWYDGEWQFIGNGNALSNCMVGNY
                     YLLWVDTSSSTRVNLITVTQPEKLSFSSILIAENDSVATLEISVSGGVPPYSVSVDSV
                     I"
     gene            complement(1545..3224)
                     /locus_tag="P5292_06025"
     CDS             complement(1545..3224)
                     /locus_tag="P5292_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRS38720.1"
                     /translation="MLLLLSLGVSGQSLDVTFNPSVFNGGYNVSCNGASDGTLEAIIV
                     GGQPPYSFQWSTGAYTKTITNLAAGTYSISVIDAAQDTITKNYTLIASEALTGSLDVS
                     TYGGGFNVSAQGAADGWITALIGGGATPYTYQWSNGAETETNHDLPSGSYSVIVRDVN
                     QCQLQLSTSLTQPTPLHIVSITSPLHNGYNLSCKSSDDGAIDLTVSGGVAPYTYQWSN
                     GNFTQDLADLKAGEYTVLVKDLNGVGVTASITLTQPVSIEVELTAPTYPNGHHTTCYN
                     CSNGSVTTTVTGGVMPYTYSWNTGQNTQNLSNLMAGTYSVLVTDANGCTKPDVKIDLT
                     EPDRDDWTMMGNTTITSGVGILGTLDTSSISIQTNNTERVLIDKDGVVSLRGRLQLFE
                     SSTDSTRDVFVNQDGELAIYNPANNGVPCLRPTNNWIQNGCDSGSIVFLKDSSISVAV
                     GANSVPAGFKLAVGGKMIAEEVRVQLKSQWPDFVFEDSYTLMPISLLKEYIDEHKHLP
                     DLPSGGEIEREGIDTGKLLNIAFKKIEELSLYIIEQNERISKLENELSDLK"
BASE COUNT          869 a          827 c          763 g          818 t
ORIGIN      
        1 cgataacgct atcaactgaa actgaatagg gcggcacccc tccgcttacg ctgatttcca
       61 aggttgcaac ggagtcattc tccgcgatca agatactcga aaagcttagt ttttcaggct
      121 gggtcaccgt tatcaaattc acacgggtac tggaacttgt atcaacccaa agcaagtagt
      181 agttgccgac catacaattg ctaagagcat tgccgtttcc aataaactgc cattcgccat
      241 cgtaccaggc cggatttccc acaaacaagg aatccaaaat aagtatttct ccgtcctgac
      301 caccataaca actcggcccg ctcacctgaa ttcccaaatc cgcgacattt atgggtccaa
      361 ggtaaatggt cctttgctga aaacacccaa cactatctgt tacgctaacc attctaagtc
      421 catttactgc cacaacctga ttcgatgaaa ttggcgctcc atccaattgg taagcatacg
      481 gcggatatcc gccgtcggaa ttcagggtca ggagacttga atcagtcgat gaggcggatg
      541 gctcttgggc gaaggatagt gtcagcggta cccgatctgg aatgttaatc gtttgaacag
      601 agacacatcc acttgtgtct tcgaacacaa aatcgtgagt cccggcagcg tagcgatagg
      661 gcatttcatc gcacgtgttt accggaacgg cttcatcctg agccggtgaa aggaagttca
      721 gtattaccgt gctcggatca tcacacagaa agagcggagt gatcagggtg ctaaactgcg
      781 cgctcatact atcgcttaac gaaagtgtcg tatctaccat gcaacccttt gcatcaaaca
      841 ccacaagatc gtatagtccg cccggaattc cggtcaaaat tgaatcctct gagtcatagc
      901 caatccaatc ataaatatac ggtccgctac cgccggtaca agacaggctg atggatccgt
      961 tggacaaatt gcaggtggga ctaacccgat gggcatcaat aaccagttca gcaggctcag
     1021 ctatttgaac tgagccggtt gtaatacagt tcgaggtgtc aaatatcgtg taggaatatg
     1081 tacccgcttt cctgccgttc atgaaacttc caacctgacc aaggttatcc catttgaagt
     1141 aatatggctc ccgcccgccg gaagaaaaga gttgcacagc tccgtttgtt ccgccgttac
     1201 aagaaacctg tgtgacagta gcggttgatt gcaatgtagg gcaattggca attcctaaaa
     1261 cgtacttccc acacaattca ccggcggatg caacgaatcc ttcccgaagt tgtatgtact
     1321 ccgtagattg aatcaccagt gcacgggcag tatctgtaaa gacaaaattc ccagtagagg
     1381 gcaaagaaac aggactgtta aacaggtagt tggtgctatc ctgataaatc gtatcgatcc
     1441 tgggttctgt ataccattgt gaatatgctg atggaatata acttgtgtaa cagcatagta
     1501 agaaaataaa attcaagcga attttcatgg cgcatcaatt gaggtcattt caaatcggaa
     1561 agttcatttt caagttttga aatcctttca ttctgctcaa tgatgtacaa ggacaattcc
     1621 tctatcttct taaaagcaat attcaacaat ttgccggtat caattccctc tctttcaatc
     1681 tcacccccgc ttggcaggtc cggcaaatgt ttatgttcat caatatactc cttcaacaaa
     1741 ctgatcggca tgagggtata agaatcttca aagacaaagt cgggccattg cgactttaat
     1801 tgtacacgca cttcttcagc aatcatcttc ccgcctacag ccaatttgaa accagccggc
     1861 actgagtttg cgcctacggc cactgaaatt gaggagtctt tcaaaaacac aatactaccg
     1921 gaatcacatc cattctgaat ccaattattc gtaggtctaa ggcatggaac cccattgttg
     1981 gcgggattat aaatagccag ttctccatcc tgattaacaa aaacatcccg ggttgaatca
     2041 gtgcttgatt caaaaagctg taatcgccct cttagtgata caactccgtc tttgtcaatt
     2101 aaaacacgct ccgtgttatt ggtttgaatg gaaatactgg atgtatccag ggtgccaaga
     2161 attccaacac cagaagttat tgttgtatta cccatcatcg tccaatcatc ccgatccggc
     2221 tccgtcaggt cgatcttgac gtcgggtttg gtgcaaccgt tggcatccgt aaccagcacc
     2281 gagtaggtgc cggccatgag gttggagagg ttctgggtgt tctgaccggt gttccaggaa
     2341 taggtgtacg gcatcacccc gccggtgacc gtggtggtca cactgccgtt ggagcagttg
     2401 taacaggtcg tgtggtggcc gttggggtag gtcggggctg tcaattccac ctcgattgaa
     2461 acgggttgcg tgagcgtgat actggccgtt acgccaacac cgttcaggtc cttgaccagc
     2521 accgtgtact cgccggcttt caggtctgcc aggtcctgtg tgaaatttcc attactccat
     2581 tgataggtat aaggcgcaac accgccggaa accgtcaggt caatggcgcc atcatcgctg
     2641 ctcttgcaac tgaggttgta cccgttgtgc agcgggctgg tgatgctgac aatatgcaaa
     2701 ggcgtgggct gcgtcagact ggtcgacagt tgcaactggc attggttgac atcccggaca
     2761 atcaccgaat acgatccgct cggcaggtcg tgattcgtct ccgtctcggc tccattgctc
     2821 cactgatagg tatagggcgt cgcgccgccg ccgatgagag cggtgatcca gccgtcggca
     2881 gccccttgcg cggaaacgtt gaatccgcca ccataagtcg aaacatcaag tgaaccggtc
     2941 agggcctccg aggcaatcag cgtatagttc ttggtgatgg tgtcctgcgc agcatcaata
     3001 acggaaatgg aatatgtacc ggctgccagg ttggtaatgg tcttcgtgta tgcacccgtt
     3061 gaccactgaa aagaataggg tggctgtccg ccaacaatga tcgcctccag ggtaccatca
     3121 ctggctccgt tgcaactcac attatatccg ccattgaaca cggaagggtt aaatgtcaca
     3181 tccaatgact gtccggagac tccgagcgac aacagtaata acacaaggga aaatcccccc
     3241 cccttaaaat attcgaaaaa cgaagtttat tcgtatt
//
LOCUS       DAOTON010000253         3269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_23123_length_3269_cov_3.653080, whole genome shotgun sequence.
ACCESSION   DAOTON010000253 DAOTON010000000
VERSION     DAOTON010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23123_length_3269_cov_3.653080"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2686)
                     /locus_tag="P5292_06030"
     CDS             complement(<1..2686)
                     /locus_tag="P5292_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38721.1"
                     /translation="MQTELSFEKGMSQDSWLQPPGTFYTSSCFSLVDPDRNLYALNSH
                     RGNEHAFSLTKNYRPVGGCARGNYIAIFAAKPGRAGGEIGRLSFDRALRTAAYTPIYN
                     NQELPFFIDRTIESDLFIENEEMVRLIWTDRVLTPKTLNFADSRFYTNIPSGSLIAST
                     EYMVVSGEILYNGVTYGPNASSGLTSFIANATAIYGLLSLSALVIQPPPLRMMDWTPE
                     KQTGNLTVVGPIPGNVPCGTGVFTYQLIDDRGAASSWSPVTFPVRFAGPDPVGYTFQA
                     YQQHQGHPFDTNSSQGVRLRVDRIDLGYTRIRIAFVRGTDYQVYEQPVVIFDSGITGS
                     SMTFDYTGAELLAQLTEEDIREINVLLKRVKSFTIINNLLIPANISKAQDVRWDPSAG
                     VTAKNIEYECLTDVMSPVTALNGPTYGAWATAGIPGTSGTTFDRIWPAQWYKVVGGPI
                     IYCGTTYSPGQYFKGIPIQTNAAGEQYTSASGGFVKPVILIKKYDNLGSPVYQEIELN
                     NDFYDGKGMTASHYVSSLWRDEKYRYFLYLTDAAGNIGYMRWLQDKTVPAQHKLASDI
                     DPDTGQPIGFDMGLVKQYALGPADWTAGRARWTARHIGTEFNGINFNNLCAELGISLA
                     DLPKYFSGFGIARCPRDGKIIAQGIMYLCTESQTGSPRTIYPIGTEKAGDDENYTVGN
                     RKPFVYAFYSPDHQLRFNGRPDLSLAEFAETAAYIFPDLMTGTWYECTGRGRDYMYKY
                     GRYDTVPAGAVGFGNKSRIDQANSLDEVEYGSSFGMLGTVFDNRGYSAGFSQANGDDS
                     YGARCSVLLLDNQNKVPLDPWSLTKPIINLISNKQVFYGGDSEAAKASNQAILTPHYQ
                     AFDSDFISMLIANSGICNGIQVFGGDAHIGFYGFERMIH"
     gene            complement(2664..>3269)
                     /locus_tag="P5292_06035"
     CDS             complement(2664..>3269)
                     /locus_tag="P5292_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38722.1"
                     /translation="IVVLDVQDCYSEPLPCSAVAVLGVIAGDKGCDCTLLFKNAFSNY
                     SSQSIAPNAQYGFYVLDGGSMQCTPNLQWRITDGRIHFINGTYRAGKITVKILRQQEE
                     DGFIKIVEHHKYAIAEFIRWMWMKRNRTDPKGKLYYQGEIAAAQQEWSRLCANATATD
                     GEPTPDQINYIRALYNDPTSGLAYASWIYQDPYYYANRIVF"
BASE COUNT          845 a          803 c          773 g          848 t
ORIGIN      
        1 catggatcat tctttcaaag ccatagaaac caatgtgagc atcgccgccg aaaacctgga
       61 ttccattaca aattccacta tttgcaatca gcattgaaat aaagtcagaa tcaaatgcct
      121 ggtaatgagg cgttaatatt gcctgattgc tggcttttgc agcttcgctg tcgccgccat
      181 aaaaaacctg tttgttggaa atcaggttga taattggctt ggttaaagac catggatcca
      241 aagggacttt attctgattg tcaagcaaaa gcacggaaca tcgtgctcca taactatcat
      301 ctccgttggc ctgactaaag ccagcagaat agccccgatt gtcaaagacg gttcccaaca
      361 tgccaaaaga agagccatat tcgacttcgt ccaggctgtt tgcctgatcg atccggcttt
      421 tattgccaaa gcctactgcc ccggcaggaa cagtgtcgta acggccgtat ttgtacatgt
      481 agtctcgccc gcggccagtg cactcatacc atgtcccggt catcaaatcc ggaaaaatgt
      541 aggctgcagt ttcggcaaac tcagcaaggg aaagatccgg gcgcccattg aacctgagct
      601 ggtgatcagg tgaatagaaa gcgtaaacga aaggcttccg attgcctaca gtatagtttt
      661 catcatcacc cgctttttct gtcccaattg gataaatggt ccttggtgaa ccggtctggc
      721 tttcagtgca cagatacata attccttggg caatgatttt gccgtctcgc gggcatctgg
      781 ctattccaaa accgctgaag tatttaggca aatccgccaa ggatatcccc aattctgcac
      841 acaggttgtt gaaattgatt ccgttaaact cagttccaat gtgccgggca gtccagcgtg
      901 cacggcctgc agtccagtct gccgggccaa gagcatactg ctttacaagc cccatgtcaa
      961 agccgatcgg ctgcccggtg tcgggatcga tatctgaagc aagcttatgt tgtgccggca
     1021 ctgttttgtc ctggagccaa cgcatgtatc ctatgttgcc ggcagcatcg gtcaggtaaa
     1081 gaaagtaacg atacttttcg tctcgccaca agctgctgac ataatgactg gcagtcattc
     1141 ctttgccatc ataaaagtcg ttattcagct caatttcctg atatacaggg gatcccaaat
     1201 tgtcatattt tttgatcaga atcaccggct ttacaaaacc accggaagca ctggtgtatt
     1261 gctcgccagc cgcatttgtc tgaattggaa ttcctttgaa atattgccct ggagaatagg
     1321 tcgttccgca gtatatgatc ggcccaccaa caaccttgta ccattgtgcc ggccagatcc
     1381 ggtcaaatgt agttccgctt gtcccgggaa tgccagccgt cgcccaggct ccataagtag
     1441 gtccgttcag tgcagtcacc gggctcatga cgtcggttag gcattcatac tcgatatttt
     1501 ttgcggtaac gccagctgaa ggatcccaac gcacatcttg ggctttcgaa atatttgcag
     1561 gaatcaacag gttgttgatg atggtaaaag acttcacccg cttcaataac acattgattt
     1621 cccgaatgtc ttcttcggta agttgagcca aaagctctgc tccggtgtaa tcaaaagtca
     1681 tggacgaccc ggtgattcca ctatcaaaaa tgactaccgg ctgttcataa acctggtagt
     1741 ctgttccccg gacaaaagcg attcgaatcc ttgtgtatcc cagatcgatc ctgtctacgc
     1801 gtaaccgtac gccctgactg gaatttgtgt caaaagggtg gccttgatgc tgctgatatg
     1861 cctggaaggt atagccgacc ggatccgggc cggcaaaccg gactgggaag gttactggcg
     1921 accaggatga cgcggcgccg cgatcgtcaa tgagttggta ggtaaaaact cctgttccac
     1981 atggaacatt gccaggtata ggccctacga ctgtaaggtt gccggtttgt ttttcgggag
     2041 tccagtccat catacgcaaa ggaggtggct ggatgaccag tgcagaaagg ctcaataaac
     2101 cataaatggc tgtcgcgttt gcaatgaagg aagtcaagcc tgagcttgca ttgggcccgt
     2161 aagtcacgcc gttatacaag atctctcccg aaaccaccat gtattcggtg ctggcaatta
     2221 aagatccgga tggaatgttg gtataaaagc gagaatcagc aaaatttagc gtttttggcg
     2281 taagaacccg atccgtccag atcagccgaa ccatctcttc attttcaata aaaaggtcgc
     2341 tttcgatggt ccgatcgata aaaaatggga gctcttggtt gttgtaaatc ggcgtgtagg
     2401 cggctgttcg aagtgcacga tcaaaggaca atcgaccgat ttccccgccg gcacgtcctg
     2461 gcttggcagc aaaaatggcg atatagtttc ctcgtgcaca gccgcctact ggccggtaat
     2521 tcttggtaag tgaaaacgca tgctcgtttc ctcggtgact gttcagcgca tagagattcc
     2581 gatccggatc caccagagaa aaacagcttg atgtgtaaaa cgtaccagga ggctgcaacc
     2641 agctatcctg agacattcct ttctcaaaag acaattctgt ttgcatagta gtacgggtct
     2701 tgatatatcc aggaggcgta agccaatccg ctggtaggat cgttgtaaag cgcgcgaatg
     2761 taattgattt ggtcaggggt aggctctccg tccgttgcgg ttgcatttgc acacaacctg
     2821 gaccattctt gctgtgcagc agcaatttct ccttggtagt acagctttcc tttgggatca
     2881 gtgcgatttc gcttcatcca catccaccga atgaattcag caatcgcata cttgtgatgc
     2941 tctactatct tgataaaacc gtcttcttcc tgctgccgaa gaatttttac tgtaatcttt
     3001 ccggcccggt acgtaccatt gataaaatga atacggccat ctgtgatcct ccattgaaga
     3061 tttggagtac actgcatgga tcctccatca agcacgtaga atccatactg agcatttggg
     3121 gcaatggatt gagaagaata gttcgagaaa gcatttttga aaagcaacgt acagtcacat
     3181 cctttgtctc ctgcaattac gcccagtaca gccacagcag aacaaggaag tggctcgctg
     3241 taacaatctt gtacgtcaag cacgacaat
//
LOCUS       DAOTON010000254         3253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_23262_length_3253_cov_3.575047, whole genome shotgun sequence.
ACCESSION   DAOTON010000254 DAOTON010000000
VERSION     DAOTON010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23262_length_3253_cov_3.575047"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..669)
                     /locus_tag="P5292_06040"
     CDS             complement(202..669)
                     /locus_tag="P5292_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRS38723.1"
                     /translation="MNLLDLLLALILLIGLVKGFINGFIYELAVVASFFLGIYLGWRL
                     ADTVAPKVASMLDTSPTTTHYIAFFLVFLAVSVGIFFLAKLLEGLIGIAALGIFNKLL
                     GALFGFGKYLLITSVALYFFHKADAKFHWLKADTKAESVLYYRVLKVAPALWK"
     gene            complement(887..1606)
                     /locus_tag="P5292_06045"
     CDS             complement(887..1606)
                     /locus_tag="P5292_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRS38724.1"
                     /translation="MSAFNKWIFGGLGWALGGPIGGILGFALGAITEESGKTYKAEPQ
                     RMLPNDFSAALLVLCAAVMKADQKVMRSELEFVRQFFARQFGEQITQDRMLLFREILK
                     QDIAIGAVCGQVRQMVDPPSRLQLLHLLFGLAASDGQVSKPELTVLEQIAHLIGIAGK
                     DFESIKAMFVSDPDAAYRILEIERTVSDDEVKKAYRRMAMKYHPDKVHHLGPEYQKDA
                     QEKFKKINEAYEEVKRERGMK"
     gene            complement(1608..2756)
                     /locus_tag="P5292_06050"
     CDS             complement(1608..2756)
                     /locus_tag="P5292_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRS38725.1"
                     /translation="MLRSRFSRFLLVLLLIAGLFPVRVADAQKSTGTPVDVVFCLDLS
                     SSANTLIDHFRNHLWDYWRFFARCKPRPNFRIGVVAYSRFSYGKPSGYSKVIKDLGTD
                     FERLSSILFKIPSRIEKGDQYVGSALKTCLSKVSWSQDPDAIKMIFLVGNGDVNLGGE
                     SVDKAVERLRQQEVMIHTIYCVAPGERKAVRGWQRIAQYSGGKHDAISLRTRYFDNVA
                     GFNLQRFRALNRKFNATYLYYGIGGAKRWRMQTEEDNHIYVTNTEGYRFRALYKISDD
                     YQQKNADWDLVDLYYRNPVAFMQVDRKTLNDSCRKMTNAQLKSYIIYKKYERKKLAAL
                     IADMVTEKELKDREAGRLTQKRMPTLDVVSLRMLREILVPRGIQCPEY"
     gene            2853..3203
                     /locus_tag="P5292_06055"
     CDS             2853..3203
                     /locus_tag="P5292_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38726.1"
                     /translation="MESDELELLRTRWDRLVKRMADQFGEEPDLQSMLFLIGVQELGR
                     GPMKYSKDEKQDLMHIAVCRVLSPYGYYRLEGQDPEGWPIWELVQKLPPLTLREQDIL
                     LKQAVVEYFGISEQ"
BASE COUNT          673 a          903 c          973 g          704 t
ORIGIN      
        1 cgcggaaatg atccatcagg tatgaggcat gaaatggccg tagatcggtc cccgatgagt
       61 cgatgccggg acgttttgtt tcagtaaaca gtgaaaagtg aacagtgaat agaattttaa
      121 caatacaacg tacgggagag ctattttaac acgaaacccg aaactcggaa cacactcgtc
      181 cctcggtctc gtccctcgtc cctacttcca cagcgccggc gctaccttca acacccggta
      241 atacaagacc gactccgcct tggtgtccgc tttgagccag tggaattttg catcggcctt
      301 gtgaaaaaag tagagggcca cgctggtgat gagcaggtac ttgccgaagc cgaacagggc
      361 gccgagtagt ttgttgaaga tccccagtgc cgcgatgccg atcagtcctt ccagcaactt
      421 cgccaggaag aagatcccca ccgataccgc caggaatacc aggaagaatg cgatgtaatg
      481 cgtcgtggtc ggggaagtgt ccagcatcga cgcgactttc ggagccaccg tatccgccag
      541 tcgccagccc aggtagatcc ccagaaaaaa actcgccacc accgccagct cataaatgaa
      601 accgttgatg aaccccttca ccagtccgat gagcaggatg agggcgagga ggagatcgag
      661 caggttcatg gataggaact gcttgtacgc agatttggtg ctcgggtttt gtggtttgca
      721 gtttcgggtt tccggtttcg ggtttccagt tgttattatg gagggacgcg ggatgtgagc
      781 agtgagccga aacgcaactt tcaactttca actttcaacc ttcaactttc aaccttcaac
      841 cttcaacctt caactaaccc gaaacccgaa actcgaaacc ggaaactcac ttcatccccc
      901 gctcccgctt cacctcctca tacgcctcgt tgatcttctt gaacttttcc tgggcgtctt
      961 tctggtattc gggaccgagg tggtggactt tatcgggatg gtatttcatc gccatgcggc
     1021 gataggcttt cttgacttcg tcgtcgctga ccgtgcgttc gatctcgagg atgcggtagg
     1081 ccgcgtcggg atcggagacg aacatggctt tgatgctctc aaaatctttt ccggcgatgc
     1141 cgatcaggtg ggcgatctgt tccagcacgg tgagctccgg cttggagact tgtccgtcgg
     1201 acgcggccag gccgaacagc aggtgcagca gttgcaggcg ggagggcggg tccaccatct
     1261 ggcgtacctg tccgcagacg gcgccgatgg cgatgtcctg cttcaggatc tcccggaaca
     1321 acaacatgcg gtcctgggtg atctgctcgc cgaactgacg cgcaaagaac tgccggacga
     1381 actccagctc cgagcgcatc accttctggt cggctttcat caccgccgcg cacagtacca
     1441 acagcgccgc gctgaagtcg ttcggcagca tgcgttgggg ctcggccttg taggtcttgc
     1501 cggattcttc ggtgatcgcg cccagggcga agccgaggat gccgccgatc ggtccgccca
     1561 gggcccagcc gagcccgccg aagatccatt tgttgaatgc gctcatctca gtattccgga
     1621 cattgaatgc cgcggggcac caggatctcc cgcagcatgc gcaggctgac cacgtcgagt
     1681 gtcggcatgc gtttctgggt gagtcggccc gcttcgcggt ctttcagttc tttctcggtg
     1741 accatgtcgg cgatcagcgc cgcgagtttc ttgcgttcgt atttcttgta gatgatgtac
     1801 gacttcaact gcgcgttggt catcttcctg caggagtcgt tcagcgtctt gcggtccacc
     1861 tgcatgaagg cgaccgggtt gcggtagtag agatcgacga ggtcccagtc ggcgttcttc
     1921 tgctggtaat cgtccgagat cttgtacagc gcccggaagc gatagccttc ggtgttggtg
     1981 acgtagatgt ggttgtcttc ttccgtctgc atccgccagc gtttcgcccc gccgataccg
     2041 taatagaggt aagtggcgtt gaacttccgg ttcagcgcgc ggaagcgctg caggttgaag
     2101 cccgcgacat tgtcgaagta ccgggtgcgg agcgagatgg cgtcgtgctt gccgccgctg
     2161 tattgcgcga tgcgctgcca gccgcgtacc gccttgcgtt cgccgggcgc gacgcagtag
     2221 atcgtatgga tcatcacctc ctgctggcgc aggcgctcta cggccttgtc gacgctctct
     2281 ccgcccaggt tcacgtctcc gttgcccacg aggaagatca tcttgatggc gtcggggtcc
     2341 tgcgaccagc tcaccttgct caggcaggtc ttcagcgcgg agccgacgta ctggtcgccc
     2401 ttctcgatcc gcgacgggat cttgaacagg atgctgctca accgttcgaa atcggtaccc
     2461 aggtccttga tcaccttgct gtagcccgaa ggtttgccgt agctgaagcg cgagtaggcc
     2521 accacgccga tgcggaagtt cggccgtggc ttgcagcgcg cgaagaagcg ccagtaatcc
     2581 cagaggtgat tgcggaagtg gtcgatcagc gtgttggcgc tgctggaaag gtcgaggcag
     2641 aagaccacgt cgaccggcgt gccggtggat ttttgggcgt cggcgacccg taccgggaac
     2701 agtcctgcga tgagcagcag gaccaggaga aagcgggaaa aacgggagcg aagcacaggg
     2761 tcagcgaggg tctttggcgg aacttttccg gagggcgggc aaaggtagga aacccgcgtc
     2821 aaccctgaca attggctacc tttgcgccgc gcatggaaag cgacgaactg gaactcttaa
     2881 ggacacgctg ggaccggctg gtcaagcgga tggcggatca attcggcgaa gagcccgatt
     2941 tgcagagcat gttgttcctg atcggcgtac aggagctggg acgtggtccg atgaaatatt
     3001 cgaaggacga aaaacaggac ctcatgcaca tcgccgtttg tcgggtgctc agtccgtacg
     3061 gctactaccg cctcgaaggc caggaccccg aaggctggcc catctgggaa ctcgtccaaa
     3121 aactcccgcc cctcacgcta cgcgaacagg atattttgtt gaagcaggcg gtggtggagt
     3181 attttgggat tagtgaacag tgaatagtga atagtgaata gtgaatagtg aatagcgaat
     3241 agcgaatagc gaa
//
LOCUS       DAOTON010000255         3250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_23296_length_3250_cov_2.628482, whole genome shotgun sequence.
ACCESSION   DAOTON010000255 DAOTON010000000
VERSION     DAOTON010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23296_length_3250_cov_2.628482"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /gene="ccoG"
                     /locus_tag="P5292_06060"
     CDS             complement(<1..1002)
                     /gene="ccoG"
                     /locus_tag="P5292_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRS38727.1"
                     /translation="MASQARPGDPGSFRDSVSTISKEGNRAWIYPQKPKGPLYNKRTW
                     VSIILLTFLFGTPFIKVNGHPLMLFNIIERKFILFGIPFWPQDFFLFVLAMLTFVVFI
                     ILFTVIFGRVWCGWACPQTIFMEMVFRKIEYWIEGDYTQQKALDKAPWTPDKMLKKTG
                     KYIAFFLISFVIANTFLAYIIGIDQLKLLAVDGPFAHMVGFIGLVVFTFVFFLVYTRF
                     REQACIVVCPYGRLQGVLLDRNSMVIAYDYQRGEPRGKIHKGEDRKLGDYIDCHQCVN
                     VCPTGIDIRNGTQLECVNCTACIDACDNIMDHVGFPKGLIRWASENNIANKERFRFTA
                     "
     gene            complement(1016..1993)
                     /locus_tag="P5292_06065"
     CDS             complement(1016..1993)
                     /locus_tag="P5292_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HRS38728.1"
                     /translation="MNTIYKIRLLVTGLLSLLTVTAGAQAAGDAAAQKTPAQVPQAFL
                     EPSFYIWLLVAFILVIVIIALSQAVNSLSRTIQEKSGLPAAASTAATEYRRKDTAWSK
                     LMQSLTASVPVEKEEDVMLHHNYDGIQELDNQLPPWWKWGFYFTIVFALVYLVSYHVS
                     GTGKLQLAEYQDAMKEAEQAKEARMKASADFVTEANVVALTEAGAIASGLEVYTKNCV
                     ACHGDKGQGTVGPNLTDDFWIHGGGIKNIFRTVTEGVPAKGMISWKSQLSPKQIQEVG
                     SFILTLHGTNPPGAKEPQGDKWIEAGAAPSDSSSAVPADSAAAPAVAAK"
     gene            complement(2009..2197)
                     /locus_tag="P5292_06070"
     CDS             complement(2009..2197)
                     /locus_tag="P5292_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HRS38729.1"
                     /translation="MFKNYFKGIEGIDSYPLFLLIVFFAFFVGLTFWLFWKADNKEMQ
                     ELGEMPLHDSITNDTTQP"
     gene            complement(2204..>3250)
                     /gene="ccoO"
                     /locus_tag="P5292_06075"
     CDS             complement(2204..>3250)
                     /gene="ccoO"
                     /locus_tag="P5292_06075"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HRS38730.1"
                     /translation="ILYWLIPRLYRTNLHSRKLANFHFWMATLGILFYAIPLYWAGFT
                     QSLMWKQFTPDGVLKYPNFLETVVQILPMYKLRVVGGTLYIIGSFVMVYNLVKTMGAG
                     SFVANEDAEAPALEKVAPEDKGHWHRWIERRPVQMLLLSLVMILIGGLVEIIPMLTIK
                     SNIPTIASVKPYTPLELEGRDIYIREGCYTCHSQMIRPFRSETERYGEYSKSGEFVYD
                     HPFQWGSKRTGPDLAREGGRYPNSWHFNHFMDPSTMSPGSIMPSYMFLIENDLSTANT
                     AAKIKTMQSLGVPYPEGYADQAGADLKKQSDMIAADLKKNGIEVDPGKEVIALIAYMQ
                     RLGKDIKASETASR"
BASE COUNT          737 a          856 c          890 g          767 t
ORIGIN      
        1 ggccgtaaac cggaatcgct ccttgttggc gatattgttc tcactggccc agcggataag
       61 tcctttcgga aatcccacat ggtccatgat gttgtcacac gcatcgatgc aggctgtaca
      121 gttcacacac tccagctgag taccgttccg gatatcgata cccgtcgggc agacattcac
      181 gcactggtgg cagtcgatgt aatcgcccaa cttcctatcc tcgcctttat ggatcttgcc
      241 acgcggttct ccgcgctggt agtcgtaggc gatgaccatc gaattacggt caagcagaac
      301 tccctgtaac cggccgtagg gacaaacaac gatacaggcc tgctcccgga agcgggtata
      361 gacaaggaag aagacaaacg tgaataccac cagcccgatg aagcccacca tgtgggcgaa
      421 agggccgtcc actgccagta acttcaactg gtcgatgccg atgatatagg ccaggaaagt
      481 attcgcaatg acgaaggaga tcaggaagaa ggctatgtac ttacctgtct tcttcagcat
      541 cttgtccggc gtccagggcg ctttgtcgag ggccttttgc tgggtgtaat cgccctcaat
      601 ccagtattcg atcttccgga agaccatttc catgaagatg gtttgtgggc aggcccatcc
      661 gcaccatacg cgaccgaaga tgaccgtgaa caggatgatg aagacgacga acgtcagcat
      721 cgccagcacg aagaggaaga aatcctgcgg ccagaatgga ataccgaaga gaatgaactt
      781 ccgctcgatg atattgaata acatcagcgg atgtccgttc accttgatga acggtgtacc
      841 aaagaggaag gtcagcagga tgatgctgac ccatgtcctc ttattataaa ggggaccttt
      901 cggtttctgg ggatagatcc aggcccgatt cccttcttta ctaatggtcg agacgctgtc
      961 tctgaatgaa cccggatcgc ccggtcgagc ttgagatgcc atgattactg ttcacttact
     1021 tcgccgccac cgctggcgca gccgcagagt cggccggaac tgccgacgac gaatcggaag
     1081 gcgctgctcc tgcttcaatc catttatcgc cctgcggttc ttttgctccg ggcggattgg
     1141 ttccatgtaa ggtaagaatg aaactgccta cttcctgaat ctgcttgggt gagagctggc
     1201 tcttccaact gatcatacct ttagccggta ccccctccgt aaccgtgcgg aaaatattct
     1261 tgatgcctcc gccatggatc cagaaatcgt cggtgaggtt cggaccaacc gtaccctgcc
     1321 ccttatcgcc atgacaagcg acgcaattct tcgtatagac ttcaagtccc gacgcaattg
     1381 cccccgcctc ggtcaaagca acgacgttgg cttccgtcac aaagtcagcg ctggctttca
     1441 tgcgcgcttc ctttgcctgc tcggcttcct tcatggcgtc ctggtactcg gccaactgaa
     1501 gttttccggt gccgctgacg tggtaactca cgaggtacac caaggcgaac acgatggtga
     1561 agtaaaatcc ccatttccac cagggcggca attggttgtc caattcctgg atgccgtcat
     1621 aattatgatg taacatgaca tcctcttcct tctcgaccgg gacggaagcg gtgagcgact
     1681 gcatcagttt actccaggcc gtgtccttgc gacgatattc cgtggcggcc gtgcttgcgg
     1741 cagccggcag gccggacttt tcctgaatgg tcctgctgag cgagtttacc gcttgagaca
     1801 gggcgatgat cacaatgaca agaatgaacg cgaccaacag ccagatgtaa aaggatggct
     1861 ctaggaaggc ctgtggaacc tgtgccggag ttttctgtgc ggcagcatct ccggcggctt
     1921 gcgcaccggc ggtgaccgtc agcaggctca gcaaacctgt aacgagtaac cggatcttat
     1981 agatggtgtt catatgcggg atggttgttt acggttgtgt ggtatcgttg gtgatggaat
     2041 cgtgcagcgg catttcgccg agttcctgca tttctttgtt atcggctttc cagaagagcc
     2101 agaaggtcag cccgacaaag aaggcgaaga atacgatcag caggaacagc ggatagctgt
     2161 cgatgccttc gattcctttg aaatagttct tgaacatgac ggatcagcgt gaagcggttt
     2221 cagaagcttt gatatcctta ccaaggcgtt gcatgtaagc gatcaaggcg atcacttctt
     2281 ttccaggatc cacttcgatg ccgtttttct tgaggtccgc ggcgatcata tcggattgct
     2341 ttttcagatc cgcgccggcc tggtcggcat atccttccgg atacggcaca cccagactct
     2401 gcatggtttt gatcttcgcg gccgtattgg cggtgctgag atcgttttcg atcaggaaca
     2461 tgtaggacgg catgatcgaa ccgggcgaca tcgtggacgg atccatgaag tggttaaagt
     2521 gccaggagtt cggatagcga ccgccttcgc gtgccagatc cggtcccgta cgtttggagc
     2581 cccactggaa cgggtggtcg tacacgaatt cacccgattt ggagtattca ccgtagcgtt
     2641 cggtttcgga acggaacggg cggatcatct gggagtggca ggtgtagcaa ccttcgcgga
     2701 tgtagatgtc gcggccttcc agttcgagcg gcgtatacgg cttgactgaa gctatggtcg
     2761 ggatattcga tttgatggtc agcatcggga tgatctcgac cagtccaccg atcaggatca
     2821 tcaccaggct gagcaacagc atttgaaccg ggcgacgttc gatccaacgg tgccagtgac
     2881 ccttgtcctc cggagcgact ttctccaatg ctggtgcttc cgcatcttca ttggcgacaa
     2941 agctgcctgc acccatggtt ttaacaaggt tgtaaaccat gacgaacgat ccgatgatgt
     3001 agagcgtacc acccaccaca cgcagtttgt acatcggcag gatctgtact acggtttcga
     3061 ggaagttcgg gtatttcaat acgccgtccg gagtgaactg cttccacatc agcgattgcg
     3121 taaagcctgc ccagtaaagc ggaatcgcat agaagaggat gccgagggtg gccatccaga
     3181 agtggaaatt ggccagtttg cgtgaatgca ggttggtccg atagagtctt gggatcaacc
     3241 agtacaggat
//
LOCUS       DAOTON010000256         3242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_23357_length_3242_cov_2.728899, whole genome shotgun sequence.
ACCESSION   DAOTON010000256 DAOTON010000000
VERSION     DAOTON010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23357_length_3242_cov_2.728899"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="P5292_06080"
     CDS             <1..914
                     /locus_tag="P5292_06080"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRS38731.1"
                     /translation="SWKKRLLRVFGWLATAVVFGALIMLVAYNVFDSPKEKRLKRQLE
                     ESTLQLELLKQRADQVEAVLGDLQERDNTIYRVIFEAEPIPQSVREAGFGGAERYRNL
                     RSDYNGELLVDVTKRIDKLSKQLYVQSKSFDEVWDLVKNKNNMLASIPAIQPVANKDL
                     TRVASGYGMRIHPIYKTEKMHTGMDFTSPVGTEIHATGNGVVNKVEYDGRGYGNNVII
                     SHGFGYQTLYGHMSKILVRPGQKVSRGDLIGYVGNTGTSTGPHLHYEVLKGGKPVNPV
                     NYYYNDLTPEEYQTMLEISSKAGQSFD"
     gene            1023..1274
                     /locus_tag="P5292_06085"
                     /pseudo
     CDS             1023..1274
                     /locus_tag="P5292_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526062.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            1562..>3242
                     /locus_tag="P5292_06090"
     CDS             1562..>3242
                     /locus_tag="P5292_06090"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRS38732.1"
                     /translation="MKLLTQRHSFLLILLLVNILSARAGEGMWMVQSASSTIGNQLRK
                     AGMILSPDSIYQEHASSLKDAVVQFGSNCTGEVVSSQGLVLTNHHCGYSQIQALSTLE
                     RNYLDSGFWARSLEAELPCPGLTVTFIREVRNVTMQVLEAIGDTMSEEDREKAVRAVS
                     DILIEGVNAETGLTSYVRSAYQGNAYFLYVTEVFRDIRLVGTPPESIGNFGSETDNWV
                     WPRHTGDFAVFRIYAGKDNQPAPFAADNVPYRPKRWFTIQTNGVQEGDFTMVVGFPGK
                     TNAYLPYSALRTVYDQTNPNRIALRQARLSAWDAAMSANDTVRLQYAAKNRTLANAYK
                     KWKGELIGMRRNETLSRVQREERDFRQWLAGNKHLPASYRQCLDELNLAYAELRPLSH
                     ASDYYTEGLFGIELMNTSLSFQPLLKAVEADSSVDAQQRLANKLIKNLEAFYRNYHAP
                     LDNLVARRLLATTFQALPDHLRAPAFGDGRWINERGVDSLYQHSILTHPDSLFTLLKN
                     WTPAFGAMLSTDPAISFARSVSSFQAEKIDRPTTLIQRRINQWQRTYMAALL"
BASE COUNT          813 a          854 c          853 g          722 t
ORIGIN      
        1 tcagttggaa gaagcgactc ctccgcgtct tcggctggct ggccacggcc gtggtgtttg
       61 gggcgctcat catgctggtt gcctataacg tctttgattc accaaaagaa aaacgtctga
      121 aacgtcagtt ggaagaatcc accttgcaat tggaactgct caaacaacga gccgaccagg
      181 tggaggctgt cttgggcgat cttcaggaac gcgacaacac gatctaccgg gtgatcttcg
      241 aagcggaacc gatcccacag tcggtgcggg aggctggctt tggaggagcg gaacgctacc
      301 gtaatctgcg gtccgattac aacggagagt tgctggtgga tgtgaccaaa cggatcgaca
      361 aactgtcgaa acaattatat gtgcagtcca aatccttcga cgaggtttgg gaccttgtga
      421 agaacaagaa caacatgctc gccagcatcc cggcgataca gcctgtagcg aacaaggatc
      481 tcacccgcgt agcttcgggc tatggaatgc ggatccatcc gatctacaaa accgagaaga
      541 tgcataccgg catggatttc acctcacctg ttgggaccga gatacacgcg acaggcaacg
      601 gtgtggtgaa caaagtggaa tacgacggac gcggctacgg gaacaatgtg atcatcagcc
      661 acggtttcgg ttaccagacc ttgtacggtc acatgagcaa gatcctcgtt cggcccgggc
      721 aaaaggtcag ccgcggcgac ttgatcggct atgtgggcaa caccggaact tctaccgggc
      781 cacacttgca ttatgaggta ctgaagggcg gtaaaccggt caatccggtc aactattact
      841 acaacgattt gacacctgaa gagtatcaga cgatgctgga aatttcttcg aaagctggac
      901 aatctttcga ctaatggcct aatggccagg gtttttcacc gtctcccggt tcataaccga
      961 ttccccggaa ccttgtaaat tccccgcccc gtcttatatt cgtaaaaaaa gaaccctgga
     1021 caatgccgta caaagagaaa gaaatcgaga agctgtacta cacgatcggt gaggtggcgc
     1081 aactgtttca ggtcaatact tcccacatcc ggttttggtc aaaggaattc gacgtcatta
     1141 aacccgcgac gaataaaaaa ggcaatcgcc tttacacgca ggcggacatc gataacttcc
     1201 ggaagatcta tcatctcgta aaggagaagg gctacaccct caaaggggca aaaacagagt
     1261 tgaaagaaca gcggcaccgc gagcgttcga atgagcccga tcaggaagcc ccggccttac
     1321 tcgatcaacc cgtcgatcag gcttctgcta tgccgtttgc cggcagcctc ttctccggcg
     1381 actatgtcga taagatggcg atccgcgact ctctgcataa aatgcgttcg accctcctcg
     1441 agctcgaccg tgagttggat gcagttctga cagcctgaag aatccgatct tacgaaaagc
     1501 gaccgccctt agcggtcgtt ttttatttac tccagccgga gtaattttat gaccatccgc
     1561 catgaagctg cttacacaac gccattcctt cctgctgatt ctgttactgg tcaacatcct
     1621 ttccgccagg gcgggagaag gcatgtggat ggtgcaatcg gcatcctcta cgattggaaa
     1681 tcagctcagg aaagccggta tgattctttc gccggattcc atatatcagg agcatgcatc
     1741 cagtctcaag gatgccgtcg tacaatttgg aagcaattgc accggcgagg tagtctcatc
     1801 tcaaggactc gtgctgacca atcaccactg cggctatagc cagatacaag ccctgagtac
     1861 actggaacgg aactacctcg acagcggatt ctgggcacgc tcccttgaag ccgaattacc
     1921 ttgtcccgga ttaaccgtaa ccttcattcg ggaagtaaga aatgtgacga tgcaagtcct
     1981 ggaggcgatc ggtgatacca tgtctgaaga ggaccgggaa aaagcagttc gcgccgtttc
     2041 tgatatcctg atcgaagggg tgaatgccga aacgggcctg acatcctatg tgcgctcggc
     2101 ttatcagggc aatgcgtatt ttctatacgt aacggaagtg tttcgtgaca tacggctcgt
     2161 tggtacgcca ccggagtcca tcgggaattt cggtagtgag accgacaatt gggtatggcc
     2221 gcggcatacc ggtgatttcg ctgtctttcg catctacgcc ggtaaggata atcagcccgc
     2281 tccttttgct gccgataatg ttccctatcg accgaagcga tggtttacga tccagacaaa
     2341 cggtgtgcag gaaggtgatt ttacgatggt ggtgggcttt cctggtaaga ccaatgccta
     2401 tctgccgtac agcgcactcc ggaccgttta tgaccaaacg aatccgaaca ggattgcctt
     2461 gcggcaggcc cgcctgtcag catgggatgc cgccatgagt gcgaacgata ccgttcgtct
     2521 tcagtacgca gccaagaacc ggacgctggc gaacgcctat aagaagtgga aaggagaact
     2581 catcggcatg cggaggaacg aaacgctttc ccgggtgcaa cgggaggagc gggacttccg
     2641 tcaatggctg gcaggaaaca agcaccttcc agcatcctac cggcagtgcc tcgatgaact
     2701 gaacctggcc tatgctgaac tgcggccgct cagtcatgcc tccgattatt atacggaagg
     2761 cttgttcggg atcgagttga tgaacaccag cctttcgttt caaccattgc tgaaggcggt
     2821 agaagccgac tcatcggtgg atgcgcaaca gcgtttggcg aataaactga taaaaaatct
     2881 ggaagccttt tacaggaact atcatgcacc gctggataat ttggtcgcaa ggcggttgct
     2941 ggccactact tttcaggcct tgccggatca tttacgcgca cctgctttcg gagacggtcg
     3001 ctggatcaat gaacggggcg tagattcgct ttaccaacat tcgatattaa cccatccgga
     3061 ttcactcttc accttattga agaattggac accggcattc ggggcaatgt tgtcgaccga
     3121 tcctgcaatc tccttcgccc gatcggtaag ctcctttcag gcagagaaga tcgatcgtcc
     3181 caccacgttg atccagcggc gaatcaacca atggcaacgg acctacatgg cggccctcct
     3241 ga
//
LOCUS       DAOTON010000257         3219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_23570_length_3219_cov_3.704488, whole genome shotgun sequence.
ACCESSION   DAOTON010000257 DAOTON010000000
VERSION     DAOTON010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23570_length_3219_cov_3.704488"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..2096
                     /gene="gldD"
                     /locus_tag="P5292_06095"
     CDS             174..2096
                     /gene="gldD"
                     /locus_tag="P5292_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HRS38733.1"
                     /translation="MDPGPDPLLLSVFSVLNPAFTFSFSFIVESVVLVVLLFLSGFVS
                     GAETAFFAITPAQFIGLRNAPGTASRIVYNLLNRPKRLLATLLIVINFINIAIVVISS
                     LLMEQVFDFSSHPLAGFLIQVVAVTFVIIVFCELMPKMYATQNAIWFSNLAALPLFVV
                     DKLLRPMSWLLVKSTALVDRRIQSKGYDVTVDELTHAIDITSDKNTPEDEKNILKGIA
                     RFGNIDVKQIMKPRMDVFAIERETPFSEVIKLVNEHRYSRVPVFEESFDRVSGILYIK
                     DLLPYLDQQDGSSFDWTSLLRPAYFVPASKKINDLLQEFQEMKNHLAIVVDEYGGSSG
                     IVTLEDILEEIVGEINDEFDDDELNYSRLDDRNFVFEGKTLLNDVCRITDLDRKSFEL
                     GEEETDTLAGFLLAVKGGLPQVNEVLRHNGLVFTIESADRRRIKRVKVTLPEIEPSNG
                     RSLAAWLLPLGFALLLSGCDEPYNPKPRGYFRITLPEKQYALWESPGCPFGSELPSYV
                     QVIPDTSAEAEPCWMNLEFPDFKATVYLTYKPVNGNLNRYIEDSRTYAYKHVTKASGI
                     DEIILHEQPGVSGLLYKVKGNAASPLQFWLTDSTAHFIRGSLYFYAVPNPDSIAPVFD
                     FVQSDVQHFLERFHWK"
     gene            2109..>3219
                     /gene="rlmD"
                     /locus_tag="P5292_06100"
     CDS             2109..>3219
                     /gene="rlmD"
                     /locus_tag="P5292_06100"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRS38734.1"
                     /translation="MKLGRRVEEFAITAIDRDGLPYGPFEGGKLYVDKSLPGDIADVQ
                     AIRVSKGDFPVSTGRIVRLLQASPDRIDPFCPHFEHCGGCQWQHTSYAHQLEIKHGQV
                     SELFARHLPDGMTVPPVLPSPQQLRYRNKIVFTFSNRRWMTPEEKTSPDTIRRPAAGF
                     LMRGKYDHTLEIHECALIDPIAVRILHRLRDIALEAGMKFYDMREEYGFLRGLALRFG
                     DPGRLLAAIITAEQDDEKLNVLTEGLTRDFPEITGYFQLFNPDKSGVNMDAPARHLRG
                     ETQLPMSLDGFQFLVSPNAFFQTNTGQTLRLYQTALDFAALKGSERVLDLYCGTGTIS
                     LFLARQAAFVTGVEFVDAAVNDARTNANRNGITNVD"
BASE COUNT          709 a          977 c          813 g          720 t
ORIGIN      
        1 cgatcgaaac cagcgcgatc cccacgggcg gcgacgacct tccgttttga ttgttaggct
       61 agtcggaaat tgttgaaagt cagctttccc ggctgccaac ccgaagtaca gctaagcgtg
      121 aagttttatt agctttgcgc ttagctgttt ttagtcaaag aagttaattc cccttggatc
      181 ccggccctga ccccctgctc ctttccgtat tctcggtcct taaccccgct tttactttct
      241 ccttttcctt tatagtcgag tcggtcgtac tggtcgtgtt gctcttcctg tccggtttcg
      301 tctccggagc ggaaaccgcc ttcttcgcca tcacacctgc ccaattcatc gggttgcgta
      361 acgccccggg gaccgcttcc cggatcgtct acaacctgct gaatcgaccc aagcgcctgt
      421 tggcgacttt actgatcgtt atcaacttca tcaacatcgc catcgtggtg atctcgtcac
      481 tgctgatgga acaggtcttc gatttctcca gtcatccgtt ggctggtttt cttatccagg
      541 tggtcgccgt caccttcgtc atcatcgtct tctgcgaact gatgccgaag atgtacgcca
      601 cccaaaatgc catctggttc tccaacctgg ccgcgcttcc gcttttcgtg gtcgacaaac
      661 tgctccggcc catgtcgtgg ctgctggtca aatcgaccgc gctggtcgac cgtcgcattc
      721 aaagtaaagg gtacgacgtg accgtcgatg agttgacgca tgccatcgac atcacctccg
      781 acaagaacac tcctgaagac gaaaagaaca tcctgaaagg catcgcccgg ttcggcaaca
      841 tcgatgtcaa gcagatcatg aagccacgga tggatgtctt cgcgatcgaa cgcgaaacgc
      901 cgttttcgga agtgatcaag ctggtgaacg aacaccggta ctcccgcgtt ccggttttcg
      961 aagaaagctt cgaccgcgtc tccggcatcc tgtacatcaa ggacctcctg ccctacctcg
     1021 accaacagga cggctccagc ttcgactgga cgagcctcct ccgcccggct tatttcgttc
     1081 cggccagcaa gaagatcaac gaccttctgc aggaattcca ggaaatgaag aaccacctgg
     1141 ccatcgtggt ggacgaatac ggcggctcct cgggcatcgt gacgctggag gacatcctcg
     1201 aagagatcgt gggcgagatc aacgacgaat tcgacgacga cgaactcaac tattcccgac
     1261 tcgacgaccg caatttcgtg ttcgaaggca agaccttgct gaacgacgtc tgccgcatca
     1321 ccgacctcga ccggaaatcc ttcgagcttg gcgaagagga aaccgatacg ctggccggat
     1381 tcctgctggc ggtgaaaggc ggactccctc aggtgaacga ggtgctgcgt cataacggac
     1441 ttgtgttcac cattgagtcg gcagatcgtc gtcgtatcaa gcgcgtaaaa gttactctgc
     1501 cggaaattga accttccaac ggaagatcgc tggcagcctg gctcctgccc ctcggtttcg
     1561 cgctcttgct gagcggttgc gacgaacctt ataatcccaa accgcgtggc tatttccgga
     1621 tcacactccc ggaaaaacaa tatgccctgt gggaaagtcc cggatgcccc ttcggctccg
     1681 agctaccctc ctatgtgcag gtgatacccg acaccagtgc cgaggcggag ccctgctgga
     1741 tgaacctgga gtttccggat ttcaaagcaa ccgtttacct cacctacaag ccggtcaatg
     1801 gaaacctgaa ccgatacatt gaagacagcc gcacctatgc ctacaagcat gtgacgaaag
     1861 cttcgggtat cgacgagatc atcctgcatg aacaacccgg cgtgagcgga ctgttgtata
     1921 aggtcaaagg caacgcggct tctcccctgc aattttggtt aacggacagt accgcccatt
     1981 tcatccgtgg ttcactctat ttctacgccg ttccgaaccc ggattcgatc gcgccggttt
     2041 tcgactttgt acaatcggat gtacagcatt tcctggaacg attccactgg aagtaattta
     2101 ctccttcgat gaaactgggt cgacgcgtcg aagaattcgc gattaccgcc atcgaccgag
     2161 acggacttcc gtacgggccg ttcgaaggag gaaaactcta cgtcgacaaa tcccttccgg
     2221 gagatatcgc cgatgtgcag gcgatccgcg tcagcaaagg cgactttccc gtctccaccg
     2281 gaaggatcgt ccgcttgtta caagcgagtc ccgatcgcat cgatccgttc tgtccgcatt
     2341 tcgaacactg cggcggttgt cagtggcagc atacctccta tgcgcaccaa ctggagatca
     2401 agcacggaca agtcagcgag ctgttcgccc ggcatcttcc cgacgggatg acggtccctc
     2461 cggtcttgcc atcaccacag caactgcgtt accgcaacaa gatcgtcttc accttctcca
     2521 accggcgttg gatgacgccg gaagaaaaga cctcgcccga taccattcgg cgtcctgccg
     2581 ccggcttcct gatgcgtggc aagtacgatc acaccctgga gatccatgaa tgcgcattga
     2641 tcgacccgat cgccgtccgc atcctccacc ggctgcgcga catcgcactc gaagcaggca
     2701 tgaagttcta cgacatgcgc gaggaatacg gcttcctgcg cggactggca ctgcgcttcg
     2761 gcgatcccgg ccgtctgctg gcggccatca tcacggcgga gcaggatgat gaaaaactca
     2821 acgtgctgac agaaggcctg acccgtgact ttcccgagat caccggttat tttcaattgt
     2881 tcaaccccga caagagcgga gtcaacatgg atgctcctgc ccgacacctg cggggtgaaa
     2941 cgcaactgcc catgagcctg gatggctttc agttcctggt cagtcccaac gccttcttcc
     3001 agacgaatac ggggcaaaca cttcgtctct atcaaaccgc attggatttc gccgcattga
     3061 aaggaagcga acgtgtgctt gacctctact gcggtaccgg aaccatcagt ctttttcttg
     3121 cacgacaggc ggcattcgtc accggggtag aattcgtcga tgccgcggtg aacgatgcac
     3181 gaacaaatgc caaccggaac ggaatcacga acgtcgatt
//
LOCUS       DAOTON010000258         3212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_23640_length_3212_cov_3.532151, whole genome shotgun sequence.
ACCESSION   DAOTON010000258 DAOTON010000000
VERSION     DAOTON010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23640_length_3212_cov_3.532151"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="P5292_06105"
     CDS             <1..830
                     /locus_tag="P5292_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HRS38735.1"
                     /translation="LTAGIDVLWIGARTTVNPFLVQEICEALRGVDIPVMVKNPVHPD
                     LELWIGALERLDQAGIHRLATIHRGFHTYEKSRYRNAPLWSLPLELKRRIPTLPILND
                     PSHICGDRALIAGVAQTAIDLGFNGLMVEVHPDPANALSDPEQQLTPDGFRTLIAGLV
                     FRKPTTDDAVVRHVLDDLRKRIDRIDDELLELLAVRMQAVREIGEYKREHNMTIFQLE
                     RWNEILRTRPATGAGLDLNKQFVVRLMELIHEDSIHQQSQILYRQPTSDGKDAAPTS"
     gene            830..1921
                     /locus_tag="P5292_06110"
     CDS             830..1921
                     /locus_tag="P5292_06110"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HRS38736.1"
                     /translation="MMDRINAGSYSIQIGADALGRLEDFARTVNKLKKFILVDANTRI
                     HCLPHLIRHCPSLSQSVVIEVPAGEACKTVESLSYVWQCMLEQGADRQSCLFICGGGA
                     VCDLGGFAAATYHRGIPCHLIPTSLLAMVDASVGGKTAINLGHVKNPVGIFSMPAGVH
                     IDPAFLSTLPTRELRSGLAEIFKHACLSSGLSFPNNRDLRSLNVTEWSEWIGLSVAYK
                     QSIVAADPEDRDVRQLLNFGHTLGHAIEAVSLSTEDDHLLHGEAIALGMIGEIYLSTL
                     LCGLPQDQAGEMVDWLCTTFHGLHWKWSVDELIRHLQHDKKRSEETVRFSLLKKRGEG
                     RVGVSVGLELVPEAVDYIRQSLARTRTVA"
     gene            1930..3144
                     /locus_tag="P5292_06115"
     CDS             1930..3144
                     /locus_tag="P5292_06115"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRS38737.1"
                     /translation="MRFHQQRVAGTVLLPASKSISNRYLLLRSLAGSDAEIANLSEAR
                     DTRLLQELLNSATTVLDAQDAGTVYRFLTAMLAYKPGKWQLTGTPRMQERPVGALVEA
                     LRTLGADIRYTGKDGFPPLEIQGGKLVSGSITVDGSVSSQFISALLLVAPFLPQGLRV
                     RTNGELVSAPYIAMTVAILRKCGIPVDVNGPDIHISAAPVRCENAVVESDWSSAAFFF
                     EAACLSPEASILFPGLEEISVQGDARLATLGSHFGLISGFDESGWRLEGTGRHPNSVE
                     LDLRGEPDLFPALCAAAAGMGMTGSFSGLGHLRNKESDRLLAMEENLLLLGADCSLVD
                     DGLVLHSAQPLRTDGILLNGFADHRIVMAMALLATRCGYIRVTDAEQVAKSFPGFWKA
                     LGKLGYEVQLEP"
BASE COUNT          677 a          786 c          939 g          810 t
ORIGIN      
        1 ccttgacggc cggaatcgat gtgttatgga tcggtgcacg aacgacggtg aatccatttc
       61 tggttcagga aatctgtgag gctttgcgag gcgtcgacat accggtgatg gtaaagaatc
      121 cggttcatcc tgacctggaa ttgtggatcg gtgcgctgga gcgattggat caggccggca
      181 tccatcggct tgctactatc caccggggat tccatacgta tgaaaaatcc cgttatcgga
      241 atgcgccgct ctggtctttg ccgctggagt tgaagcggcg tatacccact ttacccatcc
      301 tgaacgatcc cagccatata tgcggagatc gtgcattgat tgcgggtgtt gcgcagacgg
      361 ccattgacct tggattcaac ggactgatgg tggaggttca tcctgatccg gcgaatgccc
      421 tgagtgaccc tgaacagcag ttgactcccg acggcttcag gacgttgata gccgggcttg
      481 ttttccgcaa accgaccacc gatgatgcgg tggtacgtca tgttctcgat gatctgcgga
      541 agcgaatcga ccggatcgat gatgaattgt tggagttact ggccgttcgg atgcaagccg
      601 tgcgggagat tggggaatac aagcgggagc ataacatgac catttttcag ttggagcgtt
      661 ggaatgagat cttgagaacg cgtccggcaa caggtgcagg cctggatctg aataagcagt
      721 tcgttgttcg tttgatggaa ctcatccacg aagattcgat tcaccaacag agccagatcc
      781 tctaccgcca gcccacgtcg gacggcaagg atgctgcccc tacttcctga tgatggaccg
      841 gatcaatgca ggatcctatt cgatacagat cggcgcggat gcgctgggtc gattggaaga
      901 tttcgcacga actgtcaaca agttaaagaa gttcatcctc gtcgatgcga atacccgtat
      961 ccattgcctc ccgcatttga tccgacattg cccttcactg agtcagtcgg tggtaatcga
     1021 agtacccgcc ggcgaagctt gcaagaccgt tgaatcgctt tcgtatgtct ggcaatgcat
     1081 gcttgaacag ggagccgacc gacaaagctg tctgttcatt tgcggtggag gggccgtttg
     1141 cgatcttggt ggcttcgctg ccgccacgta tcatcgcgga attccctgcc acctgattcc
     1201 gacaagtttg ctcgcaatgg tcgacgcgtc cgtaggaggg aagacggcga tcaatctggg
     1261 tcatgtaaag aacccggtcg gaatattttc catgccagcc ggtgttcata ttgatcccgc
     1321 cttcctgagc accttgccaa cgcgcgagct gcgctcgggt ttggcggaaa ttttcaaaca
     1381 tgcgtgtctg tcttccggac tttcgtttcc aaacaaccgt gatctccgct ctcttaatgt
     1441 cactgaatgg agtgaatgga tcggtttatc cgtagcatac aagcaatcga ttgttgcagc
     1501 cgatccggaa gacagggatg ttcggcagtt gttgaacttc ggtcatacgc ttggtcatgc
     1561 tatcgaagcg gtttcgcttt caaccgagga tgatcacttg ctgcatggcg aggcgattgc
     1621 cctgggcatg ataggagaaa tctatctttc caccctgtta tgcggtttgc cgcaggatca
     1681 ggccggggaa atggttgact ggttatgtac gacgtttcat ggacttcatt ggaagtggtc
     1741 tgtggatgaa ttgatccggc accttcagca cgacaagaag cgctcggagg agaccgttcg
     1801 tttcagtctt ctcaagaagc ggggtgaggg ccgagtcggc gtatcggttg gtctcgaact
     1861 ggtgccggag gccgttgatt atatccggca gtcgctggca cgaacccgaa cggtcgcatg
     1921 attgcctgtg tgcgattcca tcaacagcga gtagcgggta cggtcttgct gcctgcgtcc
     1981 aagagcatat cgaaccggta tttactcctg cgatcactgg ccggcagcga tgctgagatc
     2041 gcgaatttgt ctgaagcgcg tgatacgcgt ttactgcagg aattgttgaa ctccgctact
     2101 accgtgttgg atgcacagga tgccggtacg gtttaccgct ttcttacggc tatgctagcc
     2161 tataaaccgg gcaaatggca attgacggga acgcccagga tgcaagaacg tccggtgggt
     2221 gcgttggtgg aagcactacg aaccctgggc gccgacatcc gatataccgg taaggatggg
     2281 ttcccgcccc tggagatcca aggaggaaag ttggtcagcg gatcgattac ggtggacggt
     2341 tccgtttcca gtcagttcat cagcgctctc ttgttggtgg ctccatttct gccacagggt
     2401 ctgcgagttc gaacgaacgg ggaactggta tcggcgcctt atatagcaat gacggtggcg
     2461 attctccgaa aatgcggcat accggtggat gttaatggtc cggatattca tatctccgct
     2521 gcacctgttc gttgtgaaaa cgcagtcgtt gaatcggact ggagttccgc ggcttttttc
     2581 tttgaagcgg cctgtctttc gcccgaagct tccatccttt ttcccggact tgaagaaatt
     2641 tccgttcagg gagacgcgcg cctggcaacc cttggaagtc atttcggatt gatttccgga
     2701 tttgatgaaa gcgggtggcg gctcgaagga acgggaagac atcccaattc ggtcgagttg
     2761 gatttgcgcg gggagccaga cctttttccg gcgctttgtg ctgctgctgc gggaatggga
     2821 atgaccggca gcttttcagg ccttgggcat ttgcgcaaca aagaaagcga ccgtctgttg
     2881 gccatggagg agaacctgct gttattggga gcggattgca gtttagtcga tgacggtttg
     2941 gtgctgcatt ccgctcagcc attacggact gatgggatct tgctaaacgg attcgcggat
     3001 caccgcatcg taatggccat ggcgctgctg gcaacccgat gcgggtacat tcgtgtaacg
     3061 gatgcggagc aggttgctaa atcctttccc ggattttgga aagcgctggg taagctgggc
     3121 tacgaagttc agttggaacc ttgattccag cgtaattgaa ccgagagatc gctttttatc
     3181 gtcccatccg gatgattcag tttactatct tg
//
LOCUS       DAOTON010000259         3195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_23808_length_3195_cov_3.207325, whole genome shotgun sequence.
ACCESSION   DAOTON010000259 DAOTON010000000
VERSION     DAOTON010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23808_length_3195_cov_3.207325"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..936)
                     /locus_tag="P5292_06120"
     CDS             complement(58..936)
                     /locus_tag="P5292_06120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS38738.1"
                     /translation="MLLLAPERFPDYELIDCGNFEKLERFGSVITIRPEPQAVWDPRL
                     TQRDWEKKAHLRFVPKSSSSGSWKKMKDAPDRWQIRYQLPETGGKKPAPLTFRLALTA
                     FKHVGVFPEQAVNWDYIYRSVKALNLEQPRVLNLFAYTGGASLAARAAGADVTHLDSV
                     RQVVSWARENMELSQQQDIRWLVEDAMTFVRREVKRGKKYHGIILDPPAYGHGTDGQK
                     WKLEDMINEMVQHVRALLEEKHFLVLNTYSLGFSSIILENLLADCPGLETGELYLQAT
                     CGVKLPLGVFGRVVRS"
     gene            1118..1822
                     /locus_tag="P5292_06125"
     CDS             1118..1822
                     /locus_tag="P5292_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38739.1"
                     /translation="MDNHNMPENQQQKLENQQSLIRQLTQENEELYHQFNHLMIRNNK
                     LLKRIDDLQKIIDDLHLRVEELQLISNNQLQLNDRLRRVPGGKEVSGAALPGVVAADE
                     QSLDLDLEQLYIRWKSYPGQVAQNAPNLRKQVLMLAYLYRNGSLRANELFQLTGVGGV
                     TGARYVSSLKRFGLIAFNGARKKGHYSITPQGIQFIEGKDEYRGPGGGVSMPAGIPVK
                     HALRIPDPTLMDGDDL"
     gene            complement(2065..2910)
                     /locus_tag="P5292_06130"
     CDS             complement(2065..2910)
                     /locus_tag="P5292_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38740.1"
                     /translation="MKYSLLFLGLGAALLQACSGGDDSRQQAADTAATALIDTSVTKA
                     TEQLSRKFFYTIGELPSPLELLDLINRKDIAYNSSLLNPTERADSYKTGFQKAVNYGI
                     YGVDMSYAAFYGQSQDALNYFSTTRQMAKKLGVEQPFEEFVKGVDQYQSEKDSLLSIM
                     DRAYAGIQEYLKSNHRLEVAAHVLTGSVIESQYLSLSLLQDPSAPGQEQLYQRVVDQK
                     KYVQNLIDLMSDMKEYPSSASLLNDLKGLLSAYEGINAVGDLTKEKLATLQQSVTTVR
                     NNLVK"
BASE COUNT          708 a          891 c          907 g          689 t
ORIGIN      
        1 acaacgcgaa actcgaaacc cggaacccgg aacaaccaac cctcaccggc ccatcaatca
       61 gctccgaaca accctcccaa aaacccccaa cggcaacttc actccgcagg tcgcctgtaa
      121 gtagagctcg ccggtttcga ggccgggaca atcggcgagg aggttttcga ggatgatgct
      181 ggaaaagccg agcgaatagg tgttgaggac gagaaagtgt ttctcctcga gcagtgcgcg
      241 gacgtgttgc accatctcgt tgatcatgtc ttcgagtttc catttctgtc cgtcggtgcc
      301 gtggccgtag gcgggcggat cgaggatgat gccgtggtat ttcttgccgc gcttcacttc
      361 gcggcgcacg aaggtcatcg cgtcttccac cagccagcgg atgtcctgct gctggctcag
      421 ctccatgttc tcccgtgccc aactgaccac ctgccggacg gagtcgaggt gggtcacgtc
      481 ggcgccggca gcgcgcgcgg caagtgaagc gcctccggtg taggcaaaca ggttgagcac
      541 acgcggttgc tccaggttga gcgccttgac ggagcgataa atgtagtccc agttgaccgc
      601 ctgctccgga aaaacgccca cgtgcttgaa ggcggtgagg gccaggcgga aggtcagggg
      661 cgccggcttt ttgccgccgg tttcgggaag ctgataccgg atctgccagc ggtcgggcgc
      721 gtccttcatc tttttccagc taccgctcga gctgctcttg ggtacaaacc gaaggtgggc
      781 tttcttctcc caatcgcgct gcgtcagccg gggatcccag accgcctggg gctccggacg
      841 aatggtaatg accgacccga aacgttccag tttttcaaag ttcccgcagt cgatcagttc
      901 gtaatccggg aagcgttcgg gggccaataa cagcatgggg cctaaaagta accattccgg
      961 tcaagaccgc attttttagc taaaaaacga aagttcttta ttatgagcat tttaaacata
     1021 atgatgataa aaaaatgcaa aaaggccttg cacgaatgaa aaagcgaata catttatacc
     1081 atcattaaaa ttgaaaactg cgaactttca atcccgaatg gataaccaca acatgcccga
     1141 aaaccagcaa cagaagctgg aaaaccagca gtcgctgatc cgccaactga cccaggaaaa
     1201 cgaggaactg taccaccagt tcaaccacct gatgatccgc aacaacaagc tgttgaagcg
     1261 aatcgacgac ctgcagaaga tcatcgacga cctccacctg cgcgtggaag aactccagct
     1321 gatcagcaac aaccagctcc agttgaacga ccggctacgc cgggtaccgg gcgggaaaga
     1381 ggtttccggc gcggctttgc cgggggtggt cgcggccgat gaacagtcgc tcgacctgga
     1441 ccttgagcag ttgtacatcc gctggaaatc ctatcccggc caggttgccc agaacgcgcc
     1501 caacctccgc aagcaggtac tcatgctggc gtacctctac cgcaacggct cgctgcgcgc
     1561 gaacgaattg ttccaactca ccggcgtggg cggcgtgacc ggcgctcgat acgtatcctc
     1621 gctcaaacgt ttcggtctga tcgccttcaa cggcgcgcgt aagaaaggtc actacagcat
     1681 tacgccgcag ggcatccagt tcatcgaagg aaaagatgag taccgcggtc cgggcggcgg
     1741 ggtttcgatg ccggccggca ttccggtgaa gcacgcgctg cgtatccccg atcctacttt
     1801 gatggatggg gatgatctct gataatgttc tttctaaaaa cagaaaaccc ggttacctga
     1861 tagtgaccgg gttttttatt gttttattct aggctgttgc cttactcttt atggcacgcg
     1921 gattccgcgg atcaggcgga tgaacgcgga taaagctatt ccgactatat ccgcgcaagt
     1981 ccgcaacatc cgcggcatcc gcgtgctata acttacgaag aacaaacaga ccgttggtat
     2041 cgaaaccgga ttcccggttt gacactactt caccaagttg ttccgcacgg tggttacgga
     2101 ctgttgaagc gttgccagct tttccttcgt gagatcgccc accgcgttga tgccttcgta
     2161 agcgctcagc aatcctttca agtcgttgag cagggatgcg gagctgggat actctttcat
     2221 atccgacatc aggtcgatca ggttctgtac gtacttcttc tggtccacca cgcgttgata
     2281 gagctgttcc tgtccgggtg cggacggatc ctgcagcagg gacaagctca ggtattgcga
     2341 ctcgatcacc gaaccggtga gcacgtgcgc ggccacttcg aggcggtgat tcgatttgag
     2401 atactcctgg atgccggcat acgcgcggtc catgatcgac aagagcgaat ccttctccga
     2461 ctggtactgg tccacgcctt tcacaaactc ttcgaacggc tgttcgacgc ccagtttctt
     2521 ggccatctgg cgggtggtcg agaagtagtt gagcgcgtcc tggctttgtc cgtaaaacgc
     2581 ggcataggac atgtccacgc cgtagatacc gtagttgacg gccttctgga aaccggtctt
     2641 gtaactgtcc gcccgctcgg tcgggttcag gagggagctg ttgtaggcaa tatccttccg
     2701 gttgatcagg tcgagcagct ccagcggcga aggcaattcg ccgatcgtat agaagaactt
     2761 acggctcagt tgctccgtgg ccttggtgac ggaagtgtcg atcaaagccg tcgcggcggt
     2821 atcggcagcc tgttgccggc tatcatcgcc acccgaacaa gcctgtagca gcgcagcgcc
     2881 aaggcccagg aataaaaggg agtacttcat ctcatcgaat ttggccggaa atttcggaaa
     2941 agtcgggggt gggatttggc agaaagcatg aaatcgaggt tgattgttcc gggttccggg
     3001 ttccgagttt cgggtttccg gttggaaggt tggaaggttg gaaggttgga agggacgtgg
     3061 gacgagggat gaggactggg caccgcctac tgcctactgc ctaccgtgaa ctggggcctg
     3121 tgcactgcct actgcctacc gtgaactggg gcctgtgcac tgcctactgc ctactgccta
     3181 ctgcctactg cctac
//
LOCUS       DAOTON010000260         3171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_23999_length_3171_cov_3.091784, whole genome shotgun sequence.
ACCESSION   DAOTON010000260 DAOTON010000000
VERSION     DAOTON010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23999_length_3171_cov_3.091784"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="P5292_06135"
     CDS             <1..902
                     /locus_tag="P5292_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRS38741.1"
                     /translation="WWFQYQEGGSLRNHLLNVLPLCVISSAVAIPSVRLFSKTEREFV
                     TYESSFSDILGVTFFNFISINQELTFGAFGYFALDLVFIIAISVVATLLLSILLSRIG
                     HHVKFVPIMLMVLLIYGITKSLHLPGLIFVMIFGLFVGNFNKLTRYSWAEYFRPQSLL
                     SEAHRFREITGEGAFLIRASFFILFGFLIDDKGLLNVDTLLQSGIVVGVIFSIRAVLL
                     LLTGLSLRPLLFIAPRGLITILLFLSILPDEKTGSISQSLLLQVILLSALIMTLGTLL
                     GSRKHRRTSSIPEPDENKSILNP"
     gene            915..2711
                     /locus_tag="P5292_06140"
     CDS             915..2711
                     /locus_tag="P5292_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRS38742.1"
                     /translation="MRVAFFLAILLAASCQDIVRQENQRPVYGTVRLPDAGQPAADWD
                     TTDQLLVHFTAEPDNLHPTNGTSSQRSEINLYTQGYLVQTDLRTGQLMPVLVERLPDT
                     ALNGTSFTYQLRGDVRWDDGSPLQVDDVVFTAKANKCSLTLNPGVKVYWQNVASVEAV
                     TGSPRAFRVILKKTNIQNVAFLSGFPIMERKFHDPDNVLKEVSFSQLEDPTFVSSALT
                     EWAAEFNNDRYGRDPNFLNGLGPYKVSRWEAGQSITLERKAHHWSGKPEGPQRILFRV
                     VRDENAQALEFRSQKLDVSTNCSMGAFLSLQADPELRKNYRFAVSPTYNYTYLCFNMR
                     PDSGDRPALFNDAATRRALALMTPVDRIIRLIYSDYAGACHRMSGNVSGLKPEFDTTL
                     TPLPFDPSRGREMLRQAGWLDTNGDGVLERTVNGKTVPFEAELAYLSVSPDWRDMAKV
                     IRDEWARAGIKLNLVPMELKRFQETARSHRFDLLLGSWSATSLPEDYGQLWGTASWQN
                     HGSNFSGFGSTATDVLIDSIRQSVSLSDYYRYSRSLQQTIREEQPMVFLYSSLRRVIV
                     HKRYENAIIFPERPGIRLDSLRLFTSTRKNPS"
     gene            2754..>3171
                     /locus_tag="P5292_06145"
     CDS             2754..>3171
                     /locus_tag="P5292_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38743.1"
                     /translation="MGRFLFRRLLSGCLVMLGLLLLAFVLQNALPGDPTDQWFRENDN
                     SASTQQERAAWRQRLGLDLPVFYLTLGTLADHSKTATPPTLRDETDSNWRHHVPVLQW
                     HPDNRFHRWLFGDAAFSRGIIRGDFGISWNSRQPVAD"
BASE COUNT          699 a          886 c          793 g          793 t
ORIGIN      
        1 cctggtggtt ccagtatcag gaaggaggtt cccttcggaa tcacctgttg aatgtccttc
       61 cactttgcgt tatcagcagc gccgtggcaa ttcccagtgt ccgtctattc tcgaaaacag
      121 aacgggaatt cgtcacctac gagagcagct tttcagacat tcttggagtt acattcttca
      181 atttcatttc cattaaccag gaattgacat tcggtgcatt tggctacttc gcgcttgatc
      241 tcgtgtttat tatcgccatc tcggtcgtag caacattatt actgtcgatt ctgctcagcc
      301 ggatcggcca tcatgtaaaa ttcgttccaa taatgctcat ggtgctgctg atttatggca
      361 tcacaaaaag cctgcatctt ccaggattga tcttcgtcat gattttcgga ttgtttgtag
      421 gaaattttaa caaattgact cgatactcct gggcggaata tttcagaccg caatcactct
      481 taagtgaagc gcatcgcttt cgggaaatca cgggagaggg ggcctttctg atccgggcat
      541 ccttcttcat cttgttcggt tttctgatcg atgacaaagg attactgaat gtcgatacgt
      601 tgttgcagtc cggaattgtg gtcggggtaa tcttcagtat cagggcagtc ctactcctcc
      661 tcaccggatt gtcgcttcgc cctctgctat tcatcgctcc aagaggcctt atcaccatct
      721 tgctcttcct ctcgatcttg ccagatgaaa agacgggcag catcagtcag tcattgctgc
      781 tccaggttat tctgctctcc gcattgatca tgaccctcgg gaccctcctc ggcagccgaa
      841 aacacagacg aacctcgagt attccggagc cagacgaaaa taaatcaatc ttgaatcctt
      901 agcttgcatc cgctatgcgt gttgcatttt ttctggcaat cttgttggca gcatcctgtc
      961 aggacattgt ccggcaggaa aaccaacgcc cggtgtacgg gaccgttcgt ttaccggatg
     1021 ccggtcaacc tgcggctgac tgggacacga ccgaccaatt actggtacat tttacggcgg
     1081 agccggacaa cctgcatcct accaatggga cttcctccca acggagcgag atcaacctgt
     1141 acacccaggg atacctggtc cagacggatc tccgcacggg ccagctgatg ccggttttgg
     1201 tagaacgtct gccggatacc gcgctgaacg gaacgagctt cacctaccag cttcggggag
     1261 atgtccgatg ggatgacggc tcacccctgc aagtcgacga tgtggtcttc acggccaaag
     1321 caaacaaatg ctccctgacc ctgaatccgg gtgtgaaggt ctattggcaa aatgtcgctt
     1381 ccgtggaagc ggtaactgga agcccgcgtg ccttccgcgt gattttaaag aagaccaata
     1441 ttcaaaacgt ggccttcctc agtggcttcc cgattatgga acgtaaattc catgaccccg
     1501 acaatgtgct gaaggaagtc agcttctcgc agctcgaaga tccgaccttt gtttcctctg
     1561 ccttaacgga atgggcagcc gagttcaaca acgaccgcta tggtcgggat ccgaactttc
     1621 tgaacggtct tgggccgtat aaggtgagcc gatgggaagc gggacaatcc atcacgctcg
     1681 aacggaaagc acaccattgg tctggaaaac cggaaggacc tcaacgtata ctcttccggg
     1741 tggtccgtga tgaaaacgcc caggcattgg aattccgctc acagaaactg gacgtgtcca
     1801 ccaattgctc gatgggagct ttcctttccc ttcaagccga tccggaactt cgtaaaaact
     1861 atcgattcgc tgtctcgccc acctataatt acacttatct ctgcttcaac atgcgtccgg
     1921 actcaggcga tcgaccggct ttgttcaatg acgcagctac ccggcgagcg cttgccctga
     1981 tgacaccggt cgatcggatc atcaggctga tctattcgga ttatgcaggt gcctgtcacc
     2041 ggatgtcggg caatgtatct ggcctgaagc cggagtttga caccacactc acacccttac
     2101 cattcgatcc cagtcgcgga cgggaaatgc tccggcaagc tggctggctc gacacgaacg
     2161 gcgacggcgt gctcgaacgg acagtcaatg gcaagaccgt cccattcgaa gcggaacttg
     2221 cttacctgag tgtcagtccg gattggcgcg acatggccaa agtgatccgt gacgaatggg
     2281 cacgcgcagg tatcaagctg aacctggttc cgatggaact gaagcgcttt caggaaacgg
     2341 cccgctccca ccgcttcgat ctgctgttag gaagttggtc agccacttcc cttcccgaag
     2401 actacgggca attgtgggga accgcttcct ggcagaatca tggatccaac ttcagcggat
     2461 tcggatcgac agcaaccgac gtcttgatcg attcgatccg gcaatcggtc agtctttcgg
     2521 attactaccg gtatagccgc agcttgcaac aaaccatccg ggaggaacaa cccatggtct
     2581 ttctttattc ttccctgcgc agggtcattg tacataaacg ctacgaaaac gcgatcatct
     2641 ttccggaacg tcccggcatt cgtctcgact cattgcgttt attcacaagc actcggaaaa
     2701 acccttcctg acgccctccg gctgatcgta gtggccggtt atctttgaaa tcgatggggc
     2761 gcttcctgtt tcgtcgtctg ttgtcgggtt gcctggtgat gctgggcctt ttgctgcttg
     2821 ccttcgtgtt gcagaacgcg ctgcccggtg atccgacgga tcagtggttc cgggaaaatg
     2881 acaattccgc gagtacacag caggaaaggg cggcctggcg tcaacggctt ggactggatc
     2941 ttcctgtctt ctatctcaca ctcggaaccc ttgcggatca ttcgaaaacc gctactcccc
     3001 cgacactgcg tgacgaaacc gattcgaatt ggcgtcacca tgttcccgta ctacagtggc
     3061 accctgacaa tcgttttcac cgttggctgt tcggtgatgc cgctttctcg cgcggtatca
     3121 tacggggcga cttcggcata tcctggaatt cgcgacaacc cgtcgccgat c
//
LOCUS       DAOTON010000261         3158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24119_length_3158_cov_4.579117, whole genome shotgun sequence.
ACCESSION   DAOTON010000261 DAOTON010000000
VERSION     DAOTON010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24119_length_3158_cov_4.579117"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2124)
                     /locus_tag="P5292_06150"
     CDS             complement(<1..2124)
                     /locus_tag="P5292_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38744.1"
                     /translation="MLCGNFASFTPKSLNDGYPKLNLDFSVLGNSAREDSLLGYAREH
                     HITSLSLYDLYPVFRYNDTPNSDVNGIKLVELLCNFMNKARNQYCITEIGVAGGIDSF
                     FFDVTSDTSIARQRIQYSPGLTLDSALGASIAQSDSALHFLLTNTFYQGDSLLPRAML
                     MKFFYDVLFRQSGVCNYYFDYLCYENEWWNTGGNFPTSLQDLQHMRTLEANYNATHTH
                     PLRTSVYVRSSFGGGVDTTQQAIIRQLDGTADGTLPRLADRIMAVHYGATPDGCYTNY
                     GGQIRAYADSLTIGGTDYHPYISTETFPMSGYIDYLGIWLRNKYFNNIFVEERDFYDR
                     WRVENGTNFGSASDNSLEPGGFVYFQSYFMVDSLKNPRLFRTNSPLCTNGSSGDSLRF
                     TYQGPLEQDITYELSIFDNAQTLVASDTGRTGNYPLTQTNLQLPSHYLSRNGSPYRVV
                     LTLNYGGCSYSFADSVIVLNGPSISATSPTTFCQGGNVVLQASGGTAFSWLRNGLPIA
                     VSNASTIIANQAGTYQCLITGNGTCSGSSNSIAVNVLPNPAIAFTTSCYNSDSAEIRV
                     TSPGARFSWITGDTTASIIASAAGGYRYYVTVTQSNQCQQTSYKSFSVPIVGFTANPH
                     ISTLDGTRTICAQDSFLLTPTQYCLGCLYEWNYNGSFTTTYGNYNVHPVSDSSFQISV
                     HMISASGCDGYDTATIRLYRCCPAQD"
     gene            2662..>3158
                     /locus_tag="P5292_06155"
     CDS             2662..>3158
                     /locus_tag="P5292_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HRS38745.1"
                     /translation="MDGKPGNLLKTITFPEDLRKLPEKDLDQVTRELRDYIIDVVSVN
                     GGHFGASLGVVELTVALHYVFNTPHDQLVWDVGHQAYGHKILTGRREVFHTNRIYKGI
                     SGFPKRSESPYDTFGVGHSSTSISAALGMAVASRLKGEKDRQHIAVIGDGAMTAGMAF
                     EALNNA"
BASE COUNT          771 a          805 c          845 g          737 t
ORIGIN      
        1 atcctgcgca ggacaacacc ggtagagccg gatggtcgcc gtgtcatatc catcgcagcc
       61 cgaagcgctg atcatgtgca cggagatttg aaaactggaa tccgacaccg gatggacgtt
      121 gtagttgcca taggtggtcg tgaagctgcc attgtagttc cactcgtaca ggcaacccag
      181 gcagtactgt gtgggggtta aaagaaaaga atcctgtgca cagatggtac gggtgccatc
      241 cagggtgctg atgtgcggat tggccgtaaa tccgacaatc ggtacgctga aactcttata
      301 gctggtctgc tggcattggt tggactgtgt cacggtgaca taatatcgat acccgcctgc
      361 tgcgcttgca atgatggagg cggttgtgtc tccggtaatc caggagaaac gggctcccgg
      421 agaggtgacc cggatctctg ccgaatcgct gttgtagcaa ctggtggtaa atgcgatagc
      481 agggttggga agcacattca ccgcaatgga attgctgctg ccgctgcagg tcccgttgcc
      541 cgtgatcaga cactgatatg ttccggcttg attggcgatg atggtgctgg cgttgctgac
      601 tgcaatggga aggccattcc tgagccagga gaaggccgtc ccgcccgagg cctgtagcac
      661 aacattgccg ccctggcaga atgtcgtggg cgatgtggca gagatggaag ggccattgag
      721 gacaataaca ctgtctgcaa aagaataaga gcatccgcca tagttcaggg tcaacaccac
      781 ccgataaggt gaaccgttcc ggctgaggta gtgggagggc aattgaaggt tggtttgcgt
      841 tagtgggtaa ttgccggttc ttcccgtatc ggaagcaaca agggtttggg cattgtcaaa
      901 tatgctgagt tcataggtta tgtcctgttc cagcggaccc tgataggtaa accgcaggct
      961 gtcacccgat gagccgttgg tgcaaagcgg tgagttggtc ctgaacaggc gggggttctt
     1021 cagggagtcc accatgaagt aggactggaa gtacacaaaa ccacccggct caagggagtt
     1081 gtcggaagcc gaaccgaaat tggtcccatt ctccacccgc caacgatcat agaaatcccg
     1141 ttcctccacg aagatgttat tgaagtattt attcctcagc caaatgccca ggtaatcgat
     1201 gtacccgctc atgggaaaag tctctgtgga tatatacggg tgatagtcgg tgcctccgat
     1261 ggtaagcgaa tccgcataag cccgtatctg accgccataa ttggtataac agccatccgg
     1321 tgtcgctccg taatggaccg ccattattct gtcggcaagc cgcgggagcg ttccatctgc
     1381 tgttccgtcg agttgacgta tgatcgcctg ttgagtagta tcaactcctc cgccaaagga
     1441 ggatcttacg taaacggatg tccgcaaagg atgagtgtgc gtggcattgt aattcgcttc
     1501 cagggttcgc atgtgttgca gatcctggag tgatgttggg aagttccctc ccgtattcca
     1561 ccattcgttc tcatagcaga gataatcaaa gtagtagttg cacacaccgc tctgacgaaa
     1621 caagacatcg taaaagaact tcatcagcat ggcgcgtggt agcagggagt cgccctgata
     1681 aaaggtattt gtcagcagga aatgcaaggc gctgtcgctt tgtgcaatac tcgctcccag
     1741 ggcactgtca agtgtcaggc cgggtgaata ctgaatgcgc tgccgtgcaa tagaggtatc
     1801 cgatgtcacg tcgaaaaaga acgagtcgat accgccagcc accccgatct ctgttatgca
     1861 gtattgattc cttgccttgt tcatgaaatt gcaaagcaac tcaaccagtt tgattccgtt
     1921 cacatccgaa ttgggcgtgt cgttgtaccg gaacaccgga tacagatcgt agagagacaa
     1981 agaagtaata tgatgctctc tggcgtatcc caacagcgag tcctcccgtg cactattgcc
     2041 aagcacggaa aagtcgaggt tcagtttagg gtatccgtca ttcaacgact tcggggtgaa
     2101 gcttgcgaaa ttcccgcaca gcataccccg gtttgggtca tgtgcctgac tgctggcatc
     2161 aacaggaata cacagtaaca gtaaaagcaa tcgcaaaaat gaattgaaac cggaggtaag
     2221 ttggagtgaa tgcattttga tgaaaatttt gagtttgtta gttcaatgta gaaaataaat
     2281 tgacttgacc caatcacttt tcaatcaagg gtaatgctta ttaaaacgag acgaggagtt
     2341 agcccttggc tccacttcca ttccgttaaa accctggaac aattaatttg acttacttca
     2401 aatcgggacg aatgaacttc ggtctgctga acaattcccc ttaccggctg tttcagatag
     2461 ctgttggaga aaatgaatcc tgcagccatc accctcgtcc agttatcgtc cggccctaca
     2521 ataggcgccc cgaaagctcc gtttactgcc ggtcggcacc cgcaggttca tgcctttcgt
     2581 ccatccggta agcagcctgc aacccgctcc aaacaaggac tttccccgaa ttttcctatc
     2641 tttgtagttc cccctcaaaa aatggacgga aaaccaggta atttactgaa aaccatcact
     2701 ttccccgagg atttgcgcaa gctcccggag aaggacctgg atcaggtgac ccgagaattg
     2761 cgggattata tcatcgacgt ggtttccgtg aacggaggtc attttggcgc ctccctcggg
     2821 gtcgtcgaac ttaccgtcgc ccttcattac gtcttcaata ctccccatga ccaactggtc
     2881 tgggacgtcg gccaccaggc ttacggccac aagatcctga ccggtcgccg cgaggtgttt
     2941 cataccaacc ggatctacaa aggcatcagc ggttttccga aacgttcgga aagtccgtac
     3001 gacaccttcg gcgtcggcca ttcatctacg tccatctcgg ccgccctcgg catggccgtg
     3061 gcgtcgcgcc tgaaagggga gaaagaccgt cagcacatcg ccgtcatcgg cgacggcgcc
     3121 atgaccgccg gcatggcctt cgaggcgctc aacaacgc
//
LOCUS       DAOTON010000262         3158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24124_length_3158_cov_3.346439, whole genome shotgun sequence.
ACCESSION   DAOTON010000262 DAOTON010000000
VERSION     DAOTON010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24124_length_3158_cov_3.346439"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="P5292_06160"
     CDS             <1..1825
                     /locus_tag="P5292_06160"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRS38746.1"
                     /translation="ATGILLVAAGLAVGSNITNLWATQEYGKYSTRGKSELTSEKENK
                     TSGLDRDYITDWSFGVGESLSLLVPSIKGGASEPIAKNNKDVLQKVESNFRQYVSGFG
                     AYFGEQPFTSGPVYVGAIICMLFLLGLLTIKGPEKWWLLAAFLLSITLSWGRNFMGLT
                     NFFLDFVPGYDKFRAVTTIIVIAEFAMPLLGILALDKLIREPDYFKQHAKSLYYAIGI
                     TLGLTLLIAVAPGMFTSFYSTREYEQVMATAKNQGISQDVVDNIFTNLSTARKAIVTA
                     DAWRSFLFLLLGAALIVSFARMRYKAEYFVFGLLALVLVDMWSVNARYLGDDDYVRAS
                     KGKAEAYPMTTADQVILQDPAKSYRVLNLAESTFNDASTSYRHQSIGGYHGAKLKRYQ
                     ELIENELAPELMGMRNALQNQDSSTLGVLTGQPAVNMLNTKYIVYNPDAAPLINTGAL
                     GNAWFVQNVELVANADSEIAALRGFDPSRTLVVDQRYADLVKGFTTTPDSGAFIRLTD
                     YQPNHLKYESSASTEQLAVFSEIYYDKGWNATIDGKPADYFRADYVLRAMRIPAGKHT
                     IEFRFEPRAVVVGEKIALASSSLLLLFCAGMGFLALRKRPA"
     gene            1843..3150
                     /locus_tag="P5292_06165"
     CDS             1843..3150
                     /locus_tag="P5292_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS38747.1"
                     /translation="MSAAPRKVLYITYYWPPSGGAGVQRSLKMARYLPEYGVEPIVLT
                     VDAAAATYPVEDPSLSSEVPPNLRVIRTGSFEPLQVLSRFVGGKNKIPYGGFANKDKE
                     KWTQKLLRFVRGNFFLPDARKGWVKYALREAARLIREERIDTIVVSSPPHSSQLIGLA
                     LKRSFPHVRWIADLRDPWTDIYYYPDLLHTGLAKAVDRKLERKVLEQCDHALVVSPDI
                     RRMFAAKSERVKAEKIHVIPNGFDPGDFDPAIAPPQDVFRITYVGTMAESYRPGAFFQ
                     SVAALRKQGTSLPLRLRFVGSTRAALEPILQAYGLQDIAEFIGHVPHREATRYMQESN
                     ALLLVIPDVPKANGILTGKLFEYLGAARPIIALGPVDGDAATILKETGAGRMFGREET
                     DLLSVHIHSLLSKPIAQADKLTVDLAYSRKTLAGRLAQIILAS"
BASE COUNT          653 a          851 c          945 g          709 t
ORIGIN      
        1 ggcgaccggc atcttgttgg tcgcagccgg actggctgtc ggctcgaaca tcactaacct
       61 ttgggccacc caagaatacg gcaagtactc cacccgcggg aagtcggaac tgaccagcga
      121 gaaagagaac aagaccagtg gtcttgaccg ggattatatc accgactgga gtttcggggt
      181 gggcgagagc ctgagtctgc tcgtgcccag catcaaaggg ggcgccagtg agccgattgc
      241 gaagaataac aaggacgtac tgcaaaaggt cgaaagtaac ttccggcaat acgtaagcgg
      301 cttcggcgcg tacttcggcg agcaaccgtt tacttccgga ccggtttatg tgggcgccat
      361 tatctgcatg ttattcctgc tcgggctgct gaccatcaag ggcccggaaa aatggtggct
      421 gctggcggcc ttcctgttgt ccatcacgct ttcctggggc aggaacttca tgggactgac
      481 gaattttttc ctcgattttg ttccgggcta cgacaaattc cgggcggtga ccaccatcat
      541 cgtgatcgcg gagtttgcca tgccgctgtt aggcatcctg gcactcgata agttgatccg
      601 ggaaccggat tacttcaagc agcatgcgaa aagcctgtac tatgccattg ggatcacgct
      661 gggcctgacc ttactgatag cggtcgcgcc gggcatgttc acctcgttct attcgacgag
      721 agaatatgaa caggtgatgg ctacggcaaa gaaccagggt atctcgcagg acgtggtcga
      781 caacatcttc accaacctga gcactgcacg caaggccatc gtgacggcag acgcctggcg
      841 ctcattcctg ttcctcttgt taggcgccgc actgatcgtg tccttcgcgc gtatgcgtta
      901 caaagccgag tatttcgtgt tcggtctgtt ggccctcgta ctcgttgata tgtggagtgt
      961 gaacgcgcgc tatcttggcg acgatgatta tgtgcgcgcc agcaagggta aagcggaggc
     1021 gtatcccatg accacggctg accaggtgat cctgcaggat cccgccaaga gctatcgggt
     1081 cctgaacctg gcggagagca ccttcaacga tgcgagcacg tcgtatcgtc atcagtcgat
     1141 cggcgggtac cacggcgcca agctgaagcg ctaccaggag ttgatcgaga acgagctcgc
     1201 gcccgagttg atgggaatgc gcaacgcctt acaaaaccag gattcttcga cgcttggcgt
     1261 actgaccggt cagccggccg tcaacatgct caatacgaaa tacatcgttt acaatcccga
     1321 tgctgcaccg ctcatcaata cgggtgcttt gggcaatgcc tggtttgtgc agaatgtgga
     1381 actggtagcg aatgcggatt ccgagattgc cgcgcttcgc gggttcgatc cgtcccgtac
     1441 gctcgtggtt gaccagcgct acgccgatct ggtcaaagga tttaccacga ctccggattc
     1501 cggtgccttc atccggctca ccgactacca gccgaatcac ctgaagtacg aaagctccgc
     1561 ctcgaccgag caactggcgg tattttcgga gatctattac gacaaaggat ggaacgccac
     1621 gatcgacggt aagccggctg attatttccg tgccgactac gtgttgcgtg ccatgcgcat
     1681 cccggccggc aagcacacga tcgagttccg gtttgagcca cgggcggttg tcgtgggcga
     1741 gaagatcgcg ctggcaagtt cgtcgttgct gctcctcttt tgcgccggca tgggcttcct
     1801 ggccttgcgt aaacgtcctg cctgatccgc gaccggtttg ccatgagcgc ggctcctcga
     1861 aaagtcctct acatcaccta ttactggccg cccagtggcg gagcgggcgt acagcgttcg
     1921 ctcaagatgg cgcgttatct gccggagtac ggtgttgaac cgatcgtgct gaccgttgat
     1981 gcagcggcag ccacctatcc ggtcgaggat cctagtttgt catccgaagt cccgccaaac
     2041 cttcgggtga tccggaccgg ttcctttgag cccttacaag tgctgtcgcg cttcgtcgga
     2101 ggaaagaaca agatcccgta tggagggttt gccaataagg acaaggaaaa gtggacgcag
     2161 aagttgctgc gcttcgtgcg tggtaatttt tttcttcctg acgcgcggaa aggctgggtg
     2221 aaatacgcct tgcgcgaagc cgcgcggctc attcgcgaag agcggatcga cacgatcgtg
     2281 gtttcttcgc cgccgcacag ttcccagttg atcggattgg cgctcaagcg cagctttccc
     2341 catgttcgct ggatcgcgga tctccgggat ccgtggaccg atatctatta ttatcctgac
     2401 ttgttgcata ccggtttggc gaaggcggtg gatcggaagc tggaacgaaa agtgctggag
     2461 caatgcgacc atgcgttggt cgtgagcccg gatatacggc gtatgttcgc cgccaagtcg
     2521 gagcgggtca aggcggaaaa gatccatgtg atcccgaacg gtttcgatcc gggcgatttc
     2581 gatccggcaa tcgcaccgcc acaggatgtt tttcggatca cctatgttgg aacgatggcg
     2641 gagagttatc gtccgggtgc cttttttcag tcggttgcag cattgcgaaa gcagggtacg
     2701 tcattgccgt tgcggttgcg atttgtcggc agtacacggg cggcgctgga gccgatcctg
     2761 caggcgtacg gcttgcagga cattgcggag ttcatcgggc acgtcccgca tcgggaggct
     2821 acgcgttaca tgcaggaaag caatgccttg ctgctggtta tcccggatgt accgaaggcg
     2881 aacggcatcc tgaccggaaa gctcttcgaa tacctgggcg ccgcacgtcc cattatcgcc
     2941 ttgggcccgg tcgatggaga cgccgctacg atcctgaaag agacgggagc ggggaggatg
     3001 tttgggaggg aggaaacaga tttgctgtct gttcatatcc attcgctcct gtcgaagccg
     3061 atagcgcagg ctgataagct tactgttgat cttgcttatt cacggaaaac gctggccgga
     3121 aggttggcgc aaatcattct ggcaagttga tatgaaga
//
LOCUS       DAOTON010000263         3139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24281_length_3139_cov_2.521077, whole genome shotgun sequence.
ACCESSION   DAOTON010000263 DAOTON010000000
VERSION     DAOTON010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24281_length_3139_cov_2.521077"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="P5292_06170"
     CDS             <1..1108
                     /locus_tag="P5292_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRS38748.1"
                     /translation="LDPEVLEAMLPFMQHHFGNPSSIHAFGREVRSGIEKARKTVAKL
                     LNASPSEIFFTSGGTEANNTALFCSVRDLGIQHVISSPIEHHAVTHTLEQMAREGKIR
                     LSYVKLDDKGRVDLTDLERLLAEGGKTLVSLMHANNELGNLLPIAKVATLCKQYGALF
                     HCDTVQTMGHFPIDLKEIPVDFITCAAHKFHGPKGVGFLFIRSGVSIHPFIFGGAQER
                     NMRGGTENVYGIVGLAKALELAVSHMEEHHQYIQGLKDHMVRRLKAEIPGVAFNGDTL
                     GESLYTVLNVSFPASEAGEMLLFNLDIAGISCSGGSACSSGSNAGSHVLAAIYPDSTR
                     PAVRFSFSKLNTKAEIDRTVDKLKELTVANVINS"
     gene            complement(1118..1591)
                     /locus_tag="P5292_06175"
     CDS             complement(1118..1591)
                     /locus_tag="P5292_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38749.1"
                     /translation="MNRTLLLIPAFLLLFFFSNGQPPEGRRERVEAMKIGFLTERLDL
                     SPEEAKSFWPIYNKYQDELEALRKGRRDNLINARQNFDEMSDGEVAKAVDTELQFRQS
                     ELDIIRKSDPQFSKVLPSKKVARLYQAEEAFKRKLLEMLQERREGRDGPPRGPGR"
     gene            complement(1596..1757)
                     /locus_tag="P5292_06180"
     CDS             complement(1596..1757)
                     /locus_tag="P5292_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38750.1"
                     /translation="MSVDEVSEALDYLDLDPSTVASLLPASEETGTNEQELKQYLLEN
                     DIDLSQLEL"
     gene            complement(1869..2129)
                     /locus_tag="P5292_06185"
     CDS             complement(1869..2129)
                     /locus_tag="P5292_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38751.1"
                     /translation="MKRRNQPLPHDQDPEITAGNRVLPFQTPEDYFEKLPREIERRVE
                     LLHTETARPEPDGFVAPERYFEQLESSIHARIRGGASPAFRW"
     gene            complement(2165..2713)
                     /locus_tag="P5292_06190"
     CDS             complement(2165..2713)
                     /locus_tag="P5292_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRS38752.1"
                     /translation="MTEYSDEELLEQFRQESTRHVAFNTLVRRYQQRIYWHIRKIVID
                     HDDANDVAQNVFVKVWKNLDNFRSDSKLFTWLYRIATNESISFLNQKRRRFFVPWSDV
                     ENQLSNTLHNDPYFKGDRIQLKLQQAILKLPTQQRIVFNMKYFDGMKYEDISEVLGVT
                     VGALKASYHHAVKKIEKYMLGD"
     gene            complement(2745..>3139)
                     /locus_tag="P5292_06195"
     CDS             complement(2745..>3139)
                     /locus_tag="P5292_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRS38753.1"
                     /translation="TDVSIFATGHLVWKALEACEMLEEKGISAEIINIHTIKPLDVQA
                     VLRSARKTGCAVTAEEHQVNGGLGDAIARVLVSQHPVPQEYVAVNDSFGESGTPEQLM
                     KKYGLEAENIAAAAERVIRRRKHELSST"
BASE COUNT          626 a          809 c          923 g          781 t
ORIGIN      
        1 gctcgacccc gaagtgctgg aggcgatgct gccttttatg cagcaccact tcggaaaccc
       61 ttcgtccatc cacgcgttcg ggcgggaggt gcgttcgggt attgaaaagg cccgtaagac
      121 cgttgcgaag ctgctgaacg cttcaccctc ggagattttc tttacctccg gtggtacgga
      181 agccaacaat acggccttgt tctgttcggt gcgcgacctg ggtatccaac acgtgatctc
      241 gtcgccgatc gagcaccatg cggtgaccca tacactcgag cagatggcca gggagggaaa
      301 gatccggctc agttatgtca aactcgacga caaaggacgc gtggatctga ccgatctgga
      361 gcgcttgctg gctgaaggag gaaagacgct ggtttcactc atgcatgcca acaacgaact
      421 gggcaacctg ttaccgatcg caaaagtggc tacgctgtgc aagcaatacg gcgccttgtt
      481 ccattgtgat acggtgcaaa cgatgggaca tttcccgatc gatctcaagg agatcccggt
      541 tgatttcatc acctgtgccg cgcataagtt tcacggcccg aagggcgtcg gctttctgtt
      601 cattcgctcg ggtgtcagca ttcacccgtt cattttcggt ggcgcgcagg aacgcaacat
      661 gcggggcggg acggagaacg tgtacggtat cgtcggcctg gcaaaggcgc tcgaactggc
      721 tgtttcccac atggaggagc accatcagta tatccagggc ctgaaggacc acatggtcag
      781 gcgcctcaaa gcggagatac ccggagttgc tttcaatggt gatacgctgg gagagagtct
      841 ctataccgtc ctcaacgtca gtttcccggc gagtgaagcg ggcgagatgc tgctcttcaa
      901 tctcgacatt gccggtattt cctgttcggg cggcagcgcc tgttcaagcg gatccaatgc
      961 gggttcgcac gttttggctg cgatctatcc cgatagcacc cgtccggcgg ttcggttctc
     1021 cttcagtaag ttgaatacaa aagcggagat cgatcgtacg gttgacaagc tgaaggagtt
     1081 gaccgtagcg aacgtgatca attcctgatt gagttgttta tctgcccggt ccgcgtggcg
     1141 ggccgtcgcg accttcccgg cgttcctgca acatttccag cagctttcgc ttaaaggctt
     1201 cttccgcctg gtataggcgc gcgacttttt tgctcggcaa cactttgctg aactgcggat
     1261 cggatttgcg gataatgtcg agttccgatt ggcggaattg taattcggta tccacggctt
     1321 tcgctacttc accatcgctc atctcgtcga agttctgacg ggcgttgatc aggttgtcgc
     1381 gacggccttt gcgcagcgct tccagttcgt cctggtactt gttgtagatc ggccaaaacg
     1441 atttggcttc ttccgggctg agatcaaggc gttccgtcag gaagccgatc ttcatggctt
     1501 ccacgcgctc gcgacggcct tcgggcggct gtccgttgga gaagaagaag agcaggagga
     1561 atgccgggat aagcagcagt gtacggttca tggcttcaga gttcgagttg agaaagatcg
     1621 atgtcgttct ccaggaggta ttgtttgagt tcttgttcgt ttgtcccggt ttcttcactc
     1681 gccggtaaga gactggccac tgtggaagga tcaaggtcga ggtagtccag cgcttcactg
     1741 acttcatcaa ccgacaaagg ttgaatcgct gcggtatagt cgactttccg gtcggtgaaa
     1801 tagctgcgcg ttccaaggaa caggcaaacc agcagggccg cgatcgagag ggaccattcc
     1861 ggctgcttct accagcggaa agcaggcgag gcgccacccc ggatgcgggc gtgaatcgac
     1921 gattcgagct gttcaaaata ccgttcggga gcgacgaatc cgtccggttc gggtcttgcc
     1981 gtttcagtat ggaggagctc gacccgccgc tcgatttccc gcggcagctt ttcaaagtag
     2041 tcttcgggtg tctggaacgg caatacacgg tttccggcag tgatctccgg atcctgatcg
     2101 tgtggcagtg gttggtttcg ccgtttcatg gttctgtagt tggagcggtt ctttccggct
     2161 tggtttaatc gcccagcatg tatttttcaa ttttcttgac cgcatgatgg taggaggcct
     2221 tgagggcacc aaccgtgacg ccgagaactt ccgagatatc ctcgtatttc atgccgtcga
     2281 aatacttcat gttgaagacg atccgttgct gcgttggcag tttcaaaatg gcctgctgca
     2341 gtttgagttg tatacggtca cctttgaaat aaggatcgtt gtgcagggta ttggagagtt
     2401 gattctccac atccgaccag gggacgaaga accgacgacg tttctggttg agaaacgaga
     2461 tcgattcgtt ggtcgcgatg cgatagagcc aggtgaagag tttggagtcg gaccggaagt
     2521 tgtcgaggtt cttccagact ttcacaaata cgttctgggc aacatcattc gcatcatcgt
     2581 ggtcgatgac gatctttcga atgtgccagt aaatacgttg ctggtaacgg cggaccaggg
     2641 tgttgaacgc gacgtgccgg gtcgattcct gtcggaattg ttcgagcaat tcttcatcgg
     2701 agtactccgt cataccgtgc tgggcggctg agccgcggcg gggatcaggt ggaagagagt
     2761 tcgtgcttgc gccggcggat gacccgttcg gctgcggctg cgatgttttc tgcttccaat
     2821 ccatactttt tcatcaactg ttccggcgta ccgctttccc cgaagctgtc gttgacggct
     2881 acatactcct gcggaaccgg atgctggcta accaatactc gagcgatcgc gtcgcccagg
     2941 ccgccgttga cctgatgttc ctctgcggtg acagcacatc cggtcttacg ggcagaccgt
     3001 aagactgcct gaacgtcaag gggtttaatc gtatgaatgt tgatgatttc ggcgctgatg
     3061 cctttttctt cgagcatttc gcaggcctcg agcgctttcc agacgaggtg accggtggcg
     3121 aagatgctca cgtcggttc
//
LOCUS       DAOTON010000264         3138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24289_length_3138_cov_3.139799, whole genome shotgun sequence.
ACCESSION   DAOTON010000264 DAOTON010000000
VERSION     DAOTON010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24289_length_3138_cov_3.139799"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="P5292_06200"
     CDS             complement(<1..897)
                     /locus_tag="P5292_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS38754.1"
                     /translation="MNYDLIVIGSGPGGYVAAIRAAQLGMKTAVIERSELGGICLNWG
                     CIPTKALLKSAQVYEYLLHAQDYGVKASGIEADFGAMVKRSRGVADAMSKGVQFLMKK
                     NKIEVIAGTAKIKAGRKVEALAADGKSSSYEAKHIIIATGARSRELPGLKQDGKKVIG
                     YREAMSLPQQPKSMVVVGSGAIGVEFAYFYNSIGTKVTIVEYLPNIVPLEDEDVSKQL
                     ARSFKKSGIEIMTEASVESVDTSGKGCKVNIKGKSGNSVIECDVVLSAVGITANIENL
                     GLEEVGIATDKGRIMVNDWYQTN"
     gene            1059..2792
                     /locus_tag="P5292_06205"
     CDS             1059..2792
                     /locus_tag="P5292_06205"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HRS38755.1"
                     /translation="MHYTFSAERPNSRYIDIEFTTSNPDGGEVLLQLPSWRPGRYELG
                     NFARNIQRFHVFDAAGESLPFKKLTKDSWLVHANGAESLTVRYNYYAAQPDAGACWVD
                     EEFFYINPVHCCLYVPGRLHESCSLEFRLPENYRYATSLKSTGKAVFVADDFDELVDS
                     PVLASASLQHQHYDVNGCRFHVWIQGECHPDWKRLLADFRKFTEVQVKTMVDFPFTEY
                     HFLVLALPYKFYHGVEHLRSTVLAIGPGAELMQPSVYTDFIGVASHELFHAWNVKTIR
                     PVELMPYDYTTENYSRLGFVYEGVTTYYGDLFLVRCGSYDVGQYFKEIDLRLQRHFDN
                     YGRFHTPVADASFDTWLDGYGPGIPHRKTSIYDEGSILSLMADLFIRRSTGGKRSLDD
                     VMRALYVDFGKRGIGYSERDYMSLLELTADAPMTDFFMDYVYGTENYEPLLRELLDAH
                     GCELHRSPALTVAERDFGFRIYADTMVTRVSAVAPLSIADRSGIAKEDELIAVNGQKI
                     EGNLESLLAEAGNKTELTLFSPMRRQREITLQKGNEQYYHRYNIRRKEDTSLDSELLF
                     RAWTQRSFRDA"
     gene            complement(2793..>3138)
                     /locus_tag="P5292_06210"
     CDS             complement(2793..>3138)
                     /locus_tag="P5292_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRS38756.1"
                     /translation="PIGPYSQAVKAGPFLYVSGQIALDPATGQLVTINIIREANQVME
                     NIGAILRAAGLGYEHIVKTCIFLKDMNDFGTVNEVYGEYFKGDFPARETVQVAKLPKD
                     VNVEISVIAYQS"
BASE COUNT          665 a          818 c          847 g          808 t
ORIGIN      
        1 attcgtctga taccagtcgt tcaccatgat ccggcctttg tcggtggcga tccctacttc
       61 ttcaagtccg aggttttcga tgttcgctgt aatgcctact gccgacaaaa cgacatcgca
      121 ctcgatgacc gaattgccgg attttccttt gatgttcacc ttgcagcctt tgcccgaggt
      181 gtcgaccgat tcgacacttg cctccgtcat gatctcgatg ccggatttct tgaagctgcg
      241 ggccagttgt tttgaaacgt cttcgtcttc gagcggaacg atgttcggga gatattccac
      301 gatggtgacc ttcgtgccga tcgaattata gaaataggcg aactcgacgc caattgctcc
      361 ggaaccgacg acaaccatgg atttcggttg ttgcggcagg gacatggcct cccggtagcc
      421 gatcaccttt ttgccatcct gcttgaggcc gggcaactcg cgtgagcggg cgccggtggc
      481 tatgatgatg tgtttcgctt cgtaggaaga ggacttgccg tcggcagcca aggcttcgac
      541 tttgcgcccc gctttgatct ttgcagtccc ggcgatcacc tcgatcttat tcttcttcat
      601 caggaactgg acacctttac tcatcgcatc ggcaacgcca cgactacgct tgaccatggc
      661 gccaaaatcc gcttcgatgc ccgaggcttt tactccatag tcttgtgcat gaagcaggta
      721 ctcatatacc tgtgcgctct tcaacagggc tttggtcgga atgcatcccc agttcaagca
      781 gatgccgccg agttcggaac gttcaatgac ggctgttttc attcccagtt gtgcggcgcg
      841 gatagcggca acatagcctc cgggtccgct gccgatgacg atcaggtcgt agttcatttc
      901 agttgatttt caaagcgggc cgaagatacc aattgtccgg catttgatgg gaagcgcttg
      961 tcagccaggg ggttgaaaaa aagcaagaac accctcgtcc ggtttggagg ttttcctgcg
     1021 atcttagccg gtccaatccc aagccgaaaa aaccatcaat gcactatacc ttttccgcag
     1081 agcgcccgaa ttcccggtac attgatattg agttcacgac cagtaatccg gatggaggag
     1141 aggtattgct gcaactccct tcttggcggc ccggtcgcta tgagctgggt aattttgccc
     1201 gtaatattca gcgatttcat gtcttcgatg cggctggaga atcgcttcct tttaaaaaac
     1261 taacgaaaga ttcgtggctg gtacacgcca acggcgcgga atccctgacg gtgcgttata
     1321 actattacgc ggcgcagccc gatgcaggcg cttgttgggt ggacgaagag ttcttttaca
     1381 tcaatccggt tcattgttgt ctttatgtac ccggacgttt gcacgagtcc tgttcgttgg
     1441 agttccgcct tccggaaaat taccggtatg ccaccagcct gaagagtacg gggaaggccg
     1501 tctttgttgc cgatgatttt gatgaattgg ttgattctcc ggttctggct tccgccagtt
     1561 tgcagcacca gcattacgat gtgaacggtt gtcgcttcca tgtatggatc cagggagaat
     1621 gtcacccgga ttggaagcgc ctgcttgctg attttcgaaa gttcacggag gtacaggtaa
     1681 agaccatggt tgacttcccg tttacggagt atcatttcct cgtcctggcc ttgccttata
     1741 agttctatca cggagtcgag cacctccgtt caaccgttct ggccatcgga ccgggcgctg
     1801 agttgatgca gccttcggtc tatacggact tcattggggt ggcatcgcac gaattatttc
     1861 atgcatggaa cgtcaagacg atccgtccgg ttgaactgat gccttatgat tataccacgg
     1921 aaaattattc gcgtctcggt tttgtctacg aaggggtgac cacctattat ggcgacctct
     1981 tcctggtgcg atgtgggagt tatgatgtag ggcagtattt caaagagatc gatctgcgcc
     2041 tgcagcggca cttcgataac tatggccgct ttcatacacc ggtcgctgac gcctccttcg
     2101 atacctggct ggacggctac ggccccggga ttccgcatcg aaagacatcc atttatgacg
     2161 aaggttccat cctgtcgttg atggcggact tgtttattcg ccggagtacg ggcgggaagc
     2221 ggtcactcga cgatgtgatg cgtgccttgt atgtggattt cggaaagcgc gggatcggtt
     2281 actccgaacg tgattatatg tcgttgctcg agctgacagc cgatgctccc atgaccgact
     2341 tcttcatgga ctatgtttac ggcaccgaga attacgagcc gctgctacgc gagctgctgg
     2401 atgcgcatgg ttgtgaactg catcgttcgc cggcgttgac ggtcgcagag cgtgactttg
     2461 gtttcaggat ttacgcggac acgatggtta cccgggtgag tgctgttgcg cccttgtcga
     2521 tcgcggaccg ctccggtatc gccaaagaag atgaactcat tgccgtaaac ggtcaaaaga
     2581 tcgaaggtaa tctggaaagt ctcctggcgg aagcgggaaa caagacggag cttacactct
     2641 tcagcccgat gcgtcgccag cgggaaataa cgctccagaa gggaaatgag cagtactatc
     2701 accgctataa cattcgccgg aaggaagaca cgtcactcga ttccgagttg ttgttccggg
     2761 cttggacgca gcgcagtttt cgggatgcct gatcaggatt gataggcgat aaccgagatc
     2821 tccacgttga catccttcgg caactttgcg acctgaacgg tttcacgggc cggaaaatct
     2881 cccttaaagt actctccgta tacctcattg accgtaccga aatcgttcat atccttcagg
     2941 aagatgcagg tcttgacaat atgctcgtaa ccgagtccgg cagcacgcag gatcgctccg
     3001 atgttctcca tgacctggtt ggcttcccgg atgatgttga tcgtcaccaa ttgccccgta
     3061 gcgggatcaa gggcgatctg cccggataca tagaggaaag gaccagcctt gacagcctga
     3121 ctatagggcc cgataggg
//
LOCUS       DAOTON010000265         3132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24360_length_3132_cov_1.861878, whole genome shotgun sequence.
ACCESSION   DAOTON010000265 DAOTON010000000
VERSION     DAOTON010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24360_length_3132_cov_1.861878"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="P5292_06215"
     CDS             complement(<1..165)
                     /locus_tag="P5292_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRS38757.1"
                     /translation="MNYAQAVVLAIVEGLTEFLPVSSTGHMIIASSVMGIAQESYTKT
                     FTVAIQFGAIL"
     gene            complement(221..466)
                     /locus_tag="P5292_06220"
     CDS             complement(221..466)
                     /locus_tag="P5292_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HRS38758.1"
                     /translation="MAKKETKSTIDFPFGRENYVLMLIGIVVIFIGFALMAGGGSDDP
                     AVWNPEIFSARRITVAPIVVMIGFVIEVVAIVKKSKE"
     gene            complement(518..1393)
                     /locus_tag="P5292_06225"
     CDS             complement(518..1393)
                     /locus_tag="P5292_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS38759.1"
                     /translation="MENPQDGNRRLRSSYVSAIISIALVLFLVGLLGLLILEARRLSE
                     YVREHVQVSIFLQDNLKDEQLNAFMQELESMPEVRQARYVSKEAALDSLKKELGEEAT
                     GMLEANPLPATIDLKVRAEYADPDSLRALQAHLQARSELVREVVYQQTEVERINKNFR
                     TVALVVFAFSLLLLVVAIALINNTIRLTLFSRRFTIKSMQLVGATRGFIRRPFLLRSL
                     LHGIYASLIACILLSGLYALLYARVPELAGLLDFQTLGLLFGGVVVLGMIISGVSTFF
                     AVNRYLRLHLDELYY"
     gene            1533..2981
                     /locus_tag="P5292_06230"
                     /pseudo
     CDS             1533..2981
                     /locus_tag="P5292_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
BASE COUNT          718 a          950 c          862 g          602 t
ORIGIN      
        1 caggatcgcg ccgaactgaa tggccacggt gaaggtcttg gtgtaactct cctgcgcgat
       61 gcccatcacc gaagaagcga tgatcatgtg cccggtcgaa gagaccggca ggaattcggt
      121 caggccttcg acaatagcga ggaccactgc ctgagcgtag ttcatgcaga taaggagttg
      181 atcgggtggg aaaggagcgg tcgtcgaagc gtggaacgga tcattccttc gacttcttca
      241 cgatggcaac gacctcaatg acgaatccga tcatgacaac gatcggggcc acggtaatgc
      301 ggcgtgcact gaagatttcc ggattccaaa cggcaggatc gtcggatccg ccgccggcca
      361 tcagggcgaa accaatgaaa atcaccacta taccgattag catcagtaca tagttctccc
      421 gcccgaaggg gaagtcgatc gtggatttgg tctctttctt ggccatggaa atgcggttgc
      481 ggatgcaagt atagcgtttt ccggaaaaat gccggcctta gtaatacagc tcgtccaggt
      541 gaagccgcag gtaacggttc acggcgaaaa aggtgcttac gccggaaatg atcatcccca
      601 gcaccacaac accgccgaac aacagaccca gggtttggaa atccagcaag cctgccagct
      661 cgggaacgcg ggcatagagc aaggcataga gtcccgagag caggatacag gcgatcaaag
      721 acgcatagat cccgtgcagt aaggaacgca gcagaaacgg acggcgaatg aacccgcggg
      781 tcgctccgac cagttgcata ctcttgatcg taaaccgtcg ggagaacagc gtcagacgaa
      841 tggtgttgtt gatgagcgcg atcgcgacca ccagcaacaa caggctgaac gcgaagacca
      901 ccaaggcgac cgtccggaaa ttcttgttga tgcgctccac ttccgtctgc tggtaaacca
      961 cttcccgtac cagttccgaa cgtgcctgca gatgcgcttg caatgcccgc aggctgtcag
     1021 ggtccgcgta ctccgcgcgt actttcaggt cgatcgtggc cggtaaggga ttcgcctcga
     1081 gcataccggt cgcttcttct ccgagctcct ttttcaagct gtccagcgcg gcttccttcg
     1141 aaacgtagcg tgcctgtcgt acttccggca tgctttccag ttcctgcatg aacgcgttta
     1201 gctgctcgtc tttcagatta tcctgcagga aaatactcac ctgtacgtgc tcccgcacat
     1261 actccgaaag ccggcgcgct tccaggatga gcagacccag caagccgacc aggaacagca
     1321 ccaacgcgat gctgatgatc gcggatacat aggagctgcg aagcctgcgg ttaccgtctt
     1381 gaggattttc catctggaag tgcctgcaaa agtacgcatt agcctccccg cgggagtccg
     1441 caatccgctt ccctccgccg cttttttcta cattcgcatg ttcagaaggg aagcctcgtt
     1501 tcctttctcc tcatccgcac cgatccccct ttatggagta ccccgttcgt gacatagaac
     1561 gtaaatggca ggaagcctgg aaacgcaacc aggtgtaccg cgtgaacatc gacccctccc
     1621 ggccgaagtg ttatgtgctg gatatgtttc cctatccgtc cggagcgggt ttgcatgtcg
     1681 gtcacccgct gggctatatc gcttccgata tctatgcccg ctacaagcgg ctcacaggct
     1741 tcaacgtgct ccacccgatg ggctacgatg ccttcggtct gcccgcggaa caatacgcca
     1801 tccagaccgg tcagcatccg gccgtcacca ccgagcagaa catccgccgc taccgcgaac
     1861 aactcgacaa catcggcttc tgctacgact gggaccgcga agtgcggacc tgcgacccgg
     1921 agtattaccg ctggacgcag tggatcttcc ttcaactctt caacagttgg tacaaccacg
     1981 ataccgataa ggccgagccg atcgatacgc tcatccgacg gttcgaagcg cacggctgcc
     2041 agggtcgcga ccatcgcgtt ctgaccggcg atatcgaaca tcccgtcaga cccttcacgg
     2101 cggaagaatg gaaagcgctc tccgaaaaag aacgcagcga cctcctcatg cacttccgcc
     2161 tggcctacct gggcgatgcc tgggtgaact ggtgccctgc cctcgggacc gtgctcgcca
     2221 acgacgaggt caaagacggt gtctccgaac gcggcggcta cccggtcgag cgcaagcaga
     2281 tgaaacaatg gagcatgcgc atcactgcgt atgcggaacg tttactgcag gacctcgacg
     2341 gcatcgactg gagcgactcc atcaaagaac accagcgcaa ctggatcggt aagtcggaag
     2401 gctgctcggt ctttttccag ctcgacggcc acaacggctc cgtcgaggtg ttcaccacgc
     2461 gtcccgacac gatctacggt gtcaccttcg tcacgctggc gcctgaacac gaactggtcg
     2521 accgcgtcac cacagcggaa taccgcgatg cggtcaatcg ttacgtgaat gtggcgaaga
     2581 acaagaccga acgcgaacgg caggccgaga agaaggtctc cggacaattc accggagcgt
     2641 acgtcctcca tccgttcacc ggcgcgaaag tgccggtatg ggtaggggag tacgtgcttg
     2701 ccggttacgg caccggcgcc gtcatggccg tgcctgcgca cgacgcgcgc gatcaccagt
     2761 tcgcacgcca cttcaacctg cccatcgtgc aggtcatcga agcacccgcc ggccacgata
     2821 tccaggccga gagctgggac ggtaaggaag gccgttgcat caactccggc atcatcgacg
     2881 gactcgaggt caaaacggcg atcagcacga tcatcggcga gatcgaagcc cgcggatacg
     2941 gcaaaggcaa gacgaattat cgcctgcgcg atgccgcctt cgtggaagcc gaacgcggta
     3001 gttaatgcag atagtccgac accaacgagg ctgtcagttc caagttttcc tgtcttaccc
     3061 atatcgccga acagcagatt catcagttgc gccgatgccg cttgcgcaat catcttttgc
     3121 actgtcttac cg
//
LOCUS       DAOTON010000266         3117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24507_length_3117_cov_2.524494, whole genome shotgun sequence.
ACCESSION   DAOTON010000266 DAOTON010000000
VERSION     DAOTON010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24507_length_3117_cov_2.524494"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..341
                     /locus_tag="P5292_06235"
     CDS             171..341
                     /locus_tag="P5292_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38760.1"
                     /translation="MTDGLGRVVWNKDVAVSGNSISTLSLPDLGNLQTGFYLIRVASG
                     EKEFVRKVVRSR"
     gene            complement(343..609)
                     /locus_tag="P5292_06240"
     CDS             complement(343..609)
                     /locus_tag="P5292_06240"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRS38761.1"
                     /translation="MATTQTMAWKEEDNRLTRSYEFKDFAHAFSFMTRVAFICEAMNH
                     HPVWSNSWNKVHISLTTHDAGHMVTEKDRRLSDAIEKVWDEIKG"
     gene            complement(757..1701)
                     /gene="trxB"
                     /locus_tag="P5292_06245"
     CDS             complement(757..1701)
                     /gene="trxB"
                     /locus_tag="P5292_06245"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRS38762.1"
                     /translation="MSETIEHVQCLIIGSGPAGYTAAIYAARADLKPVMYQGLQPGGQ
                     LTITTEVENYPGYPKGTHGPQMMEDFKAQAERFGTDIRFGYATSVDFTGPVHKVVIDE
                     TKTIHADSVIIATGASAKWLGLPSEQRLNGYGVSACAVCDGFFFKGQTVAIVGAGDTA
                     AEEATYLAKLCKKVYMIVRRDAMRASKAMQHRVLNTPNIEVLWNHETKEIVGEKGVEG
                     AILIDHKTGQERRIDVTGFFVAIGHHPNSDIFKGWIDMDETGYIKNIPGTSKTSREGV
                     FAAGDVADKVYRQAVTAAGSGCMAALDAERYLASKGVH"
     gene            complement(1750..2631)
                     /locus_tag="P5292_06250"
     CDS             complement(1750..2631)
                     /locus_tag="P5292_06250"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HRS38763.1"
                     /translation="MRTTRLLLVSLLLLSLSACEKNVTVDIPEAEELLVVEGYIENGQ
                     PALLALSRTLPFFGSINVTNVIQNTVQGARVIVSDGTTIDTLDQLPGFGVYVSSLIGE
                     AGKTYTLRVEAEGKVLTASTKIPEVVTLDSTWWKVDGERDSLGFVWARMTEPDTLGNC
                     YRWFAQRINRYTFGDDIGRQKDSIFIAPLGSVFEDKFINGRSFNFSFPRGIATNSEKE
                     DDENEERFFFKRGDTIVVKFTAIDRDHFEFWRTAETQIQNNGNPFGSPAPITSNISGG
                     LGIWGGYAASFDTVIAQ"
     gene            complement(2636..>3117)
                     /locus_tag="P5292_06255"
     CDS             complement(2636..>3117)
                     /locus_tag="P5292_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS38764.1"
                     /translation="GWIGYTLSWTKRKFEDLNLGKEFFAKFDRRHDINIVLTRQMGTR
                     WILGATWEYSTGNLNTFPQRLYLLSDGNIVQDYGGERNNYRLPAYHRLDFSATLKNKP
                     GRKYESSWNFSVFNVYNRYNPYIIYFDTKSENGTIEIQAKQISLFPIIPSVTWNFKF"
BASE COUNT          695 a          815 c          855 g          752 t
ORIGIN      
        1 gcctcaacgg gagaggcaag cccctgaacg cgctggctga cctgctgctg ggcggtatca
       61 tcgacggtct tccttatgaa gaagcgctga tgagcgttta tccgaatccg ttcaatgatc
      121 gcctggaact gcagtatttc tcccggtagg cacaaaccat tcggctctcg ctgaccgatg
      181 gcttgggacg ggtcgtatgg aacaaggatg tggcggtaag cgggaacagc atcagtaccc
      241 taagccttcc agacctcgga aatctccaga cgggtttcta cctcatccgg gtagcttcag
      301 gagaaaagga attcgtccgg aaggtcgtac gttcccgctg attcaaccct tgatttcgtc
      361 ccacactttc tcaatggcat ccgatagccg gcggtccttt tccgtgacca tatgtccggc
      421 atcgtgcgtt gtcagactga tgtgaacctt gttccagcta ttgctccata ccgggtggtg
      481 attcatggct tcacaaatga aagcgaccct tgtcatgaac gaaaaggcgt gcgcaaagtc
      541 cttgaactcg taactgcgcg ttagtcggtt gtcctcttct ttccaagcca ttgtttgtgt
      601 ggttgccatg atgtggtccg acaaatgtaa ccaaacgaca atccggcgca agtaagtggc
      661 atccaccaca aagcgtaaac cagcgaataa ttaaattagc attaaatagc tgattataag
      721 ctatttaatg ctaattggcg aagtggttga ttcgggtcag tggacgccct ttgacgcgag
      781 atagcgttcg gcatccaagg cggccataca tccgcttccg gctgcggtga cggcttgccg
      841 atagacctta tcagcaacat cacccgctgc aaaaacacct tcccgggaag ttttcgaagt
      901 gccgggaata ttcttgatgt agccggtttc gtccatatca atccaacctt tgaaaatgtc
      961 ggaattggga tggtgcccaa tggcaacgaa aaaacccgtg acatcgattc tgcgttcctg
     1021 gcccgtcttg tggtcgatca ggatcgcacc ttccacaccc ttctcaccga cgatctcctt
     1081 ggtctcgtga ttccagagta cttcaatgtt aggggtgttg aggacacggt gttgcatggc
     1141 cttgctggcc cgcatagcat cccggcggac gatcatgtag accttcttac aaagtttggc
     1201 gaggtaggta gcctcttccg cggcagtatc accggcaccg acaatggcga ccgtttgacc
     1261 tttgaagaag aatccgtcac aaaccgcgca agcggatacg ccgtaaccgt tcaggcgttg
     1321 ttccgagggc aggccgagcc atttggcaga agcgccggta gcgatgataa ccgagtcggc
     1381 atggatggtc ttcgtttcgt cgatgaccac cttgtgaacg ggcccggtga aatcgacgga
     1441 cgttgcatag ccgaaacgga tatccgtgcc aaagcgctca gcctgggcct tgaaatcctc
     1501 catcatttgc ggaccgtgtg ttcccttcgg gtatcccgga taattctcga cttccgtcgt
     1561 gatggtcaat tgtccgcctg gctgaagtcc ctggtacatt acaggcttca gatcggcacg
     1621 ggcggcatat atggcggcag tatatccggc tggacctgat ccgatgatca ggcactgtac
     1681 atgttcgatg gtctcactca tggcctaatg aaataggccg caaaggtacg gacaaagaac
     1741 gaacccgcct tactgagcaa tgacggtgtc aaaggaagcg gcataaccgc cccaaatgcc
     1801 caatccgccg ctgatgttgg acgtgatcgg cgccggggat ccgaacgggt tcccgttgtt
     1861 ctggatctgc gtctcagccg tacgccaaaa ttcaaaatga tcccggtcaa tggcagtgaa
     1921 tttcacgacg atcgtatcgc cgcgcttgaa aaagaaccgc tcttcatttt cgtcgtcttc
     1981 cttttcggag ttggtggcaa ttccccgcgg gaaactgaaa ttgaagctcc tgccgttgat
     2041 gaacttgtcc tcgaagacag agccgagcgg tgcgatgaat atggaatcct tttgccttcc
     2101 gatgtcatct ccgaacgtat agcggttgat gcgctgggcg aaccaacggt agcagttgcc
     2161 cagggtgtca ggctccgtca tgcgggccca gacaaaacca agcgagtccc gttcgccatc
     2221 gaccttccac caggtgctgt cgagtgtcac cacttcgggg atcttggtcg acgctgtcag
     2281 gactttccct tcggcttcga cccggagcgt atacgttttt ccggcttcac cgatcaggct
     2341 ggatacatag acgccgaatc ccggcaactg atccagcgtg tcgatcgtcg tgccatcgga
     2401 tacgatcacc cgggcgccct ggacagtgtt ctggatcaca ttggtgacgt tgatgctgcc
     2461 aaagaagggg agagtcctgg acagggcgag tagggcaggc tgcccatttt cgatatagcc
     2521 ttccaccacg agcaattctt cagcttcggg gatgtcgaca gttacgttct tctcacaagc
     2581 cgagagcgac aggagcagga gggagacaag gagtagtcgg gtcgttctca tcggttcaaa
     2641 atttgaagtt ccaggtgaca cttggaatga tcgggaagag cgagatctgt ttggcctgga
     2701 tctcgatggt cccgttttcc gacttggtgt cgaagtaaat gatgtacgga ttgtaccggt
     2761 tgtaaacatt gaacaccgaa aagttccagc tggattcgta tttgcggccg ggtttgttct
     2821 tcagcgtagc cgaaaagtcc agtcggtgat aagcaggcaa acggtagttg ttgcgttcac
     2881 cgccatagtc ctgcacaata ttgccgtctg ataagaggta caggcgttgt gggaaggtgt
     2941 tcaggtttcc ggtggagtat tcccaggtcg caccgaggat ccatcgtgtt cccatctgtc
     3001 gggtcaggac gatgttgatg tcgtgccggc gatcgaactt ggcaaagaac tctttaccaa
     3061 ggttcaggtc ttcgaatttc cgtttggtcc aggagagcgt atagccgatc cagccac
//
LOCUS       DAOTON010000267         3114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24534_length_3114_cov_3.923831, whole genome shotgun sequence.
ACCESSION   DAOTON010000267 DAOTON010000000
VERSION     DAOTON010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24534_length_3114_cov_3.923831"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="P5292_06260"
     CDS             complement(<1..320)
                     /locus_tag="P5292_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS38765.1"
                     /translation="MARFLYPAFLSILSEAVSNRTAMSGKRYLSPLLSPGIRDFFREA
                     GEITRFALRFLKEIWVPPYEFREVIRQSYQIGFRSLSLVGLTTFIIGMVLTLQSRPTL
                     VERS"
     gene            397..1674
                     /locus_tag="P5292_06265"
     CDS             397..1674
                     /locus_tag="P5292_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HRS38766.1"
                     /translation="MSKPDREAEPKAGKSRAGKFSGFWNRLGPGLITGSSDDDPSGIA
                     TYTQAGARFGLATLWTSIITFPLMAGLQEMCARIGLVTSHGLMGVIRRHYPRWISFLV
                     IVLSFPAITLNIGADLAGMGAVSTMLFPSIHPGIFSLGFAVLLVPAVILLSYNRLARV
                     LKWMCLTLLCYLVVPFFADLDWQQVVHGTFLPDVSFSKEFLFILVGILGTTISPYLFF
                     WQASVEVEEKEHRSVIVDKHVLAAVKADINYGMGFSGLVMYFIILSAGAILFPEGVRN
                     IDTVEQAAAALKPLAGRYAYLFFAVGVIGTGLLAIPVLAGSLSYMIAEAFDWKYGMDK
                     KFYEAKGFYLVLIVSVGIALLIHFSGLSAVRSLLYTAVLYGLTAPVLIGLILHICNNR
                     SIMGEYVNGRLANLLGAIALVVMTVCAGALLFY"
     gene            1677..2315
                     /locus_tag="P5292_06270"
     CDS             1677..2315
                     /locus_tag="P5292_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRS38767.1"
                     /translation="MPTEIKIILDHFPIFSEQGLKEALAEVSLLQTFPSETTLIRENE
                     PVRFLPLLIRGVVKVMRNDESGRELLLYYIRAGESCIASFNAAVFRRTSQIKAITEEE
                     SELLLVPVHALPDLMERFPTWNRFLFQLYHQRFEELLQSVNTIAFSPMEQRLELLLSR
                     KVELTGKREIRTTHQELADELGTAREVVSRLLKRMEQDGKLSLARGEIRISE"
     gene            2389..2598
                     /locus_tag="P5292_06275"
     CDS             2389..2598
                     /locus_tag="P5292_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRS38768.1"
                     /translation="MKKNMGTTDRVLRVIVAIVLAVLYYNGTITGTLGLTLIVLSVVF
                     VLTSLVGFCPLYLPFGMNTCGKEKS"
     gene            2637..>3114
                     /locus_tag="P5292_06280"
     CDS             2637..>3114
                     /locus_tag="P5292_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38769.1"
                     /translation="MDDRSQRFLLTPFASMIREEDGIQVEIPVGNSRLAGNLFLPEGA
                     DRCVIFAHGSGSSRFSPRNRRIAKLLFQSGIGSFLTDLLTASEDAVPELRFDIPLLTD
                     RLVEITRSVNAIHAMDSVRLSYFGASTGAAAALLAAGRLPELVRAVVCRGGRTDMAG"
BASE COUNT          641 a          814 c          885 g          774 t
ORIGIN      
        1 tccgatctct cgaccagtgt cggccgcgac tgcaaggtca gcaccatgcc aatgatgaag
       61 gtggtcagtc caacgagtga gagagagcgg aagccgatct ggtacgactg tcggatcact
      121 tcgcggaatt catagggcgg gacccagatc tctttcagaa agcgcagggc gaaacgcgtg
      181 atctcgccgg cttcccggaa aaaatcccga atgccgggag ataggagtgg ggacaggtac
      241 cgcttgccgc tcatagccgt tcggttggaa accgcttcgc tcaagatcga aagaaatgcc
      301 ggataaagga agcgcgccat gtcgggtatc agcgggttta ttgacaaacg tcagggtatt
      361 ggttgaagcc ccgtccgaat tagtacattc gatgcgatga gcaaaccgga ccgcgaagct
      421 gaaccgaagg caggaaaaag ccgtgccgga aagttctccg gtttttggaa ccggcttggt
      481 cccggtctga tcaccggttc gagcgacgac gatccttccg gcattgccac ctatacacaa
      541 gccggtgcac gattcggact tgcaactttg tggacctcga tcatcacttt tccgttgatg
      601 gccggcttgc aggagatgtg tgcccggatc ggattggtca cttctcacgg ccttatgggt
      661 gtgatccggc gccactaccc gcgctggatc tccttcctgg tcattgtgct tagctttcct
      721 gccataacgc tgaacatcgg tgcagacctc gccggaatgg gagccgtttc gaccatgctg
      781 ttcccatcga tccatcccgg catcttttca ttgggattcg cggtcttgtt ggtgccggcc
      841 gtgatcctcc tttcctataa ccgcctggcg cgtgtactga aatggatgtg cctcacatta
      901 ttgtgctacc tggtcgttcc gttcttcgct gatctggact ggcaacaggt tgtacacggt
      961 acgttcttgc cggatgtcag cttcagcaag gaattcctgt tcatcctggt cgggatactg
     1021 ggaaccacga tctctccata cttgtttttc tggcaggcca gcgttgaagt ggaggagaag
     1081 gagcaccgga gtgtcatcgt ggataagcat gtgctggcag ccgttaaagc ggacatcaac
     1141 tatgggatgg gcttttccgg acttgtgatg tatttcatca tcctcagtgc cggcgcgatc
     1201 ctgttcccgg aaggtgtccg gaatatcgac accgtggagc aggctgccgc tgcactgaag
     1261 ccactggcgg ggcgatacgc ttatctcttc tttgcggtcg gcgtgatcgg gaccggtctg
     1321 ctcgcgattc ccgttctcgc cggatcgtta tcatacatga ttgccgaagc cttcgactgg
     1381 aaatatggca tggacaagaa gttctacgaa gcgaagggct tttatctggt cctgatcgta
     1441 tcggtcggta tagctttgct gatccatttt tcaggactct cggccgtacg atcgctcctg
     1501 tatacggcgg tactttacgg actgaccgcg ccggtgctga tcggactcat tttgcatatt
     1561 tgcaataacc gctcgatcat gggcgaatac gtgaacggtc gattagcgaa tttgctcggc
     1621 gcgatcgcac tggtcgtgat gacggtttgt gcaggtgcac tcctattcta ttgatcatgc
     1681 cgacagaaat aaaaatcatc ctcgaccatt tcccgatctt cagcgagcag ggattgaaag
     1741 aagcattggc cgaagtgtcc ttattgcaga cctttccttc ggaaacgacc ctgatccggg
     1801 agaatgagcc cgtgcgtttt cttccgttac tcatccgggg tgtggtaaaa gtcatgcgca
     1861 acgatgagtc gggtcgggaa ctattgctgt actacatacg tgcaggcgaa agttgcatcg
     1921 cttccttcaa tgccgcggtc tttcgacgta ccagccagat aaaagcgatc acggaggagg
     1981 agtcggaatt actgttggtt ccggtccatg ctttaccgga tctgatggaa cgttttccta
     2041 cctggaaccg gtttctgttt caactttatc atcagcggtt cgaagagttg ttgcaatccg
     2101 tcaacacgat tgctttctcc ccgatggaac agcgactgga attgttactc agtcggaaag
     2161 tggagcttac cggcaagcgg gaaatacgca ccacgcacca ggagttggcc gacgagttgg
     2221 gcacggcgcg ggaggtggtg tcccggttgc tcaaacggat ggaacaggat ggaaaactca
     2281 gccttgcccg cggggagata cgcatcagcg aatgaaagcg ccagaatgtg accccggtca
     2341 ccgtctgccg gggaacacca tcctaccttg gtaccaaaat tgagcatcat gaaaaagaac
     2401 atgggtacta cggatcgtgt cttgcgggtg atcgtcgcaa tcgtactggc tgtgctttat
     2461 tacaacggta ccatcaccgg cactctcggt cttacgctga tcgtcctgtc ggtcgttttc
     2521 gtcctgacca gcctggtcgg tttctgcccg ctctatttac cgttcgggat gaatacctgc
     2581 ggtaaggaaa aatcctgacc ctccggtctc gcgcattttt ttatcttgca ttcggggtgg
     2641 atgaccgtag ccaacggttt ctcctgactc cttttgcaag tatgatccgg gaagaagacg
     2701 gtatacaagt ggaaatcccg gtcggcaata gccggctggc tggcaatctc tttcttccgg
     2761 aaggcgctga ccgctgtgtg atcttcgcgc acggctcggg aagcagccgt ttcagtccgc
     2821 gtaatcgtag gatcgccaaa ctcctttttc aatccggtat cgggagtttt ctaaccgacc
     2881 tcttgacggc ttcggaagat gccgttccgg agttgcggtt tgatataccc ctgcttaccg
     2941 acaggctggt ggaaataaca cgttccgtta acgcgattca cgcgatggat tcggtcaggc
     3001 tctcgtactt cggagcgagt accggtgcgg cggcagcctt gctagccgct ggtcggcttc
     3061 cggaattggt gcgcgcggtg gtttgtcgcg gaggacgtac cgacatggcc ggtg
//
LOCUS       DAOTON010000268         3111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24567_length_3111_cov_3.088024, whole genome shotgun sequence.
ACCESSION   DAOTON010000268 DAOTON010000000
VERSION     DAOTON010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24567_length_3111_cov_3.088024"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..2822
                     /locus_tag="P5292_06285"
     CDS             48..2822
                     /locus_tag="P5292_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRS38770.1"
                     /translation="MKRRILLLPFLLLLPFLSLAQQAVLSGTIRDESNQPVTGANVSL
                     KGTNFQMVTDSLGGFRFDKVPFGDYQLEIKRVDFAPVSQPVKVDAPESFFGTILTGVN
                     NEVTVNNADNIPTVSLNDEEVKESSSTSVASVLSAARDPFVSAATFNFSAARFRIRGY
                     DDENFLTLMNGVPMQDLATGRTMYNTWSGLNDVMRSRENSIGLSAAPFTFGGVGGAYH
                     IDSRASKQRRQLSVGYAASNRAYENRFMVTYGSGIMNKGWAYSLSYSRRWAEEGFVPG
                     TFYDGHAYYAAVEKMIGDKHSISLTGFGSSVINGRGGSAIREMYDIAGTNYYNPNWGY
                     QNGEKRNAVVGNNHQPVIILSHEWKIDDKANVQTSIGYTFGKTRLSGLDWFNAADPRP
                     DYYRRLPSFDPNYGEDPAAYAEYKAQIDALLRNNEEERQIKWDQLYAANAMADTNFNG
                     TTGKWSKYIIGDRVTDNKRLFANMIYNRVMSDHVTLDAGLQYLTQTSEYYREVNDLLG
                     GDFYVDLNQFAELSNPNDSAAAQNDLDNPNRILKEGDKYQYDYVAHINRAGAWFQSTF
                     KYDRMDYFLALNLSSTSFFREGKVRSGIFSDNSLGDSEKQNFFNYGFKGGATYKYNGR
                     NYFFANAAYMTRAPYFDNVFISPRTRNEIVKDLTDEKVFSTEAGYLLKAPRIKGKAVA
                     YYTQFMDATDSKIFYHEDFRNFVTYTLTNIDRRHVGVEIAVEAALGKGFSANAVAAVG
                     QHVYSDRPIGTAALDNADSLLVENETLYMKNLRVSNGAQSAYSFGLTYRSRRFWFVNA
                     NLNYFDNLYIDANPVRRTLAALDLVNEGDALWKQILQQEKTEGQFTLDLSGGWSWRLN
                     NKFKDLKRQTFLVLNLGVSNVLNNQDLVTGGFEQLRYDFADKNVNKFPTRYTYAFGTT
                     FFANIVLRIN"
     gene            2846..>3111
                     /locus_tag="P5292_06290"
     CDS             2846..>3111
                     /locus_tag="P5292_06290"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HRS38771.1"
                     /translation="MTSFKHLFRFFLIALTAASLSGCVKKDYDEPETANVDPDLTVDL
                     TIAQLRFMAVGTAPIEITGDSIIAGIIVADDESGTFYKQMIIQDS"
BASE COUNT          735 a          984 c          770 g          622 t
ORIGIN      
        1 gccgcccctc cgccggagat tccggtaaaa ggctataatc aaaccctatg aaacgaagga
       61 tactccttct tcccttcctg ctcctgttgc cgttcctatc cctggcacag caggcggttc
      121 tgtccggaac catccgcgac gaaagcaacc aacccgttac cggagccaat gtttcgctga
      181 aaggaacaaa cttccaaatg gttaccgatt cactcggcgg attccgcttc gacaaggtgc
      241 cattcggcga ttaccaattg gaaatcaaac gcgtcgattt cgcgccggtc agccaacccg
      301 tcaaggtgga tgcaccggaa tccttcttcg gaacgatcct caccggcgtt aacaacgaag
      361 tgacggtcaa caatgccgac aacatcccga cggtgtcgct gaacgacgaa gaggtgaaag
      421 aatcctcctc gacttccgtg gccagcgtcc tgagcgcggc tcgtgatccg ttcgtctccg
      481 cggcgacctt caacttcagt gccgcccgat tccggattcg cggctacgac gacgagaact
      541 tcctgacact catgaacggc gttcccatgc aggatctcgc caccggtcgc acgatgtata
      601 acacctggag cggtctcaac gatgtgatgc gcagccggga aaactccatc ggactttccg
      661 ccgcgccttt cacattcggc ggtgtaggtg gagcctacca catcgacagc cgtgcctcga
      721 agcagcgccg ccagctttcg gtcggttacg ccgcgtccaa tcgcgcctat gagaaccgct
      781 tcatggtaac ctacggttcc ggcatcatga acaagggttg ggcctactcc ctctcctatt
      841 cccgccgctg ggcagaggaa ggattcgttc ccggaacgtt ctacgacgga cacgcctact
      901 atgcagcggt cgagaagatg atcggcgaca agcattcgat ctcgcttacc ggtttcggct
      961 ccagcgtcat caatggccgg ggcggttccg ccattcggga gatgtacgat atcgccggca
     1021 ccaactatta caacccgaac tggggttatc agaatggtga aaagcgcaac gcagtcgtgg
     1081 gcaacaacca ccagccggtg atcattctct cccacgagtg gaagatcgac gacaaggcga
     1141 acgtccaaac ttcgatcggt tacaccttcg gcaagacgcg gctttcgggc ctggactggt
     1201 tcaacgccgc tgacccgcgt cccgattact accgccgcct cccctccttt gatccgaact
     1261 acggcgaaga tcccgcagcc tatgccgagt acaaggccca gatcgacgcg ctgcttcgta
     1321 acaacgagga agaacgtcag atcaaatggg accagttgta cgccgccaac gcgatggcag
     1381 acaccaactt caacggtacc acgggtaaat ggtcgaagta catcatcggc gatcgcgtga
     1441 ccgacaacaa acgcctgttc gccaacatga tctacaaccg tgtgatgagt gaccacgtca
     1501 ccctcgacgc gggcttgcag tacctcacgc agacttccga gtattaccgc gaagtcaacg
     1561 acctgctcgg aggcgacttt tacgttgacc tcaaccagtt cgccgaattg tcaaatccga
     1621 acgattccgc cgcagcacaa aacgacctgg acaacccgaa ccgcatcctg aaagaaggcg
     1681 acaagtacca atacgactac gtcgcgcaca tcaaccgcgc cggtgcctgg ttccagtcga
     1741 ccttcaagta cgaccggatg gattacttcc tggcgctgaa tctttcctcc acctccttct
     1801 tccgtgaagg aaaggtccgc agcggcattt tttccgataa ctcacttggc gactcggaga
     1861 aacagaattt cttcaactat ggtttcaagg gcggtgccac ttacaagtac aacggccgga
     1921 actatttctt tgccaacgcg gcctacatga cccgcgcgcc ttatttcgac aacgtcttca
     1981 tctctccccg tacccgcaac gagatcgtga aggacctgac cgacgagaag gtcttctcta
     2041 cggaagccgg ttacctgctg aaagcaccgc ggatcaaggg taaagcggtt gcgtactaca
     2101 cgcaattcat ggacgctacc gactccaaga tcttctacca cgaagacttc cgcaacttcg
     2161 tgacctatac gttgaccaac atcgatcgcc gtcacgtcgg cgttgagatc gcggtggaag
     2221 cggcgctggg caaaggattc agcgcaaatg cggtggccgc cgtcggccag cacgtttatt
     2281 ccgatcgccc gatcgggacg gctgctctgg ataatgccga ctccctgctc gtcgagaatg
     2341 agacgctgta tatgaagaac ctccgcgtat cgaacggtgc ccaaagcgcc tacagcttcg
     2401 gtctgaccta tcgttcccgc cgcttctggt tcgtgaatgc caacctgaat tacttcgaca
     2461 atctctacat cgacgccaac ccggttcgcc ggaccctggc agcgctcgac ctcgtcaacg
     2521 aaggcgatgc cctgtggaag cagatcctcc agcaggaaaa aaccgaaggt cagttcaccc
     2581 tcgacctttc agggggctgg tcctggcgac tgaacaacaa gttcaaggat ctcaagcgcc
     2641 agaccttcct ggtgttgaac ctgggcgtga gcaacgtact gaacaaccag gatctcgtca
     2701 ccggcgggtt cgagcaactc cgctacgact tcgccgataa aaacgtcaac aaattcccca
     2761 cccgttacac ctacgcgttc ggaacgacct tcttcgccaa catcgtcctg cgcatcaact
     2821 aatagaaaaa gaaaacacca aggatatgac cagcttcaag cacctcttcc gtttcttcct
     2881 gatcgcactc acggcagctt cgctgtcggg ttgcgtgaag aaagactacg acgaaccgga
     2941 aaccgccaac gtggacccgg acctgaccgt cgacctcacc atcgcgcaat tgcgcttcat
     3001 ggccgtcggc actgctccca tcgagatcac cggtgattcc atcatcgccg gtatcatcgt
     3061 agccgacgac gaatccggca cgttctacaa acaaatgatc atccaggatt c
//
LOCUS       DAOTON010000269         3105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24625_length_3105_cov_2.933115, whole genome shotgun sequence.
ACCESSION   DAOTON010000269 DAOTON010000000
VERSION     DAOTON010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24625_length_3105_cov_2.933115"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="P5292_06295"
     CDS             <1..1065
                     /locus_tag="P5292_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HRS38772.1"
                     /translation="QLLRLPPSVSVEIVQQEVTDLRREDHHWSIVTEQGRRIKAEAVV
                     LATGNSLPGTPSGLTGISESSRYCGNPWLESCVVTTHEREVLIVGTGLTMVDIVLGLR
                     GKGYTGTIIAISPKGFDILPHRNFPPHKAILDELREPYRIDQLVRVFRQHVFAISKLG
                     ISGEAVVDALRSKTQEIWQQLSSREKRQFIQHLRHLWGMARHRLPFTIHEQIQREIRK
                     GSLIIRAGRILSASEEQARIKLEIRPRGSDATETLRVDRVINCTGPQPDIRKVASPLL
                     QRLTDRGLIVPHELNLGIEATTDGRIIDSDGNAQTDFLTLGTLLRGALWESTAVPELR
                     VQAAGVARHLVNLLATRTTP"
     gene            1221..1625
                     /gene="msrB"
                     /locus_tag="P5292_06300"
     CDS             1221..1625
                     /gene="msrB"
                     /locus_tag="P5292_06300"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRS38773.1"
                     /translation="MQEQNGISKDELKKKLTPEQYRITQECGTEAPFTGKYWDNHETG
                     MYHCVVCNAPLFSSNTKYDSGSGWPSFYAPVEGSNIREKRDSSHGMIRTEIVCAKCGA
                     HLGHVFDDGPNPTGLRYCTNSGSLQFYKEGSK"
     gene            complement(1635..>3105)
                     /locus_tag="P5292_06305"
     CDS             complement(1635..>3105)
                     /locus_tag="P5292_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38774.1"
                     /translation="ARRDDTISSIYLLDLDGDNDMDALIGDISSYNTLMVHNGGTQFS
                     AEMDTQDVSFPSYDLAAVFNGFHAHSYIDLDNDGKRDMLFAPNENENRQGMWWYKNTA
                     TDPNPTFSYRSSDFLVGGMIDVGEAAVPVPFDYDSDGLLDLIVGGSIFQNPPQANKNS
                     LWLFRNKGTATQPAYELITDDLAGISALNLVAPLAATFGDLDADGDADMIIGADDGKL
                     YFFNNSAGAGNPCSFQLAFPNYMGIDVGNTATPQLFDINRDGLQDLLIGEKSATINYF
                     KNIGTAASAFYSNLPDNDTLGGILLQSQGYVEGYTVPFFYDTAGTRRLAVSNMGGNVY
                     FYDNIDGNLNGTFRLLDSLYNKPESSRIKFNVSVNGGDINGDGFVDLVLGQASGGLQL
                     WYQYNPTAGVPALEEPAVSFIAFPNPARDEVRVSFNHLSGKGTEQLEVTDIAGRVLYR
                     SRVSAEQMVISTTEWASGLYLLRLVSETASRSMKLRVQH"
BASE COUNT          698 a          954 c          848 g          605 t
ORIGIN      
        1 caactcctcc gactgccacc cagcgtgagc gtggagatcg tgcaacagga ggtcaccgac
       61 ctgcgccgcg aagaccacca ctggtcgatc gtgacggaac agggaagacg catcaaggcc
      121 gaggcggtcg tgctcgccac cggcaacagt ctgccgggaa caccctccgg actcaccggc
      181 atcagcgaga gcagccgtta ctgcggcaac ccctggctcg agtcctgcgt cgtcacaacg
      241 catgaacgcg aagtgctgat cgtcggcacc ggcctgacaa tggtcgacat cgtcctcggt
      301 ctgcgcggca aagggtacac cggaacgatc atcgccatct cgcccaaagg cttcgacatt
      361 ctcccccacc gtaattttcc tccgcacaaa gcgatcctcg atgaattgcg ggaaccctac
      421 cgcatcgatc agctcgtacg cgtcttccgg caacacgtct tcgccatcag taaactgggc
      481 atctccggag aggcggtcgt ggacgccttg cgttcgaaga cccaggagat ctggcaacaa
      541 ctcagcagcc gggaaaagcg acaattcatc caacacctgc ggcacctctg gggaatggcg
      601 cgacaccgcc tgccgttcac catccacgaa cagatccaac gtgagatccg caaaggcagc
      661 ctcatcatac gtgccggcag gatcctctcc gcatccgaag agcaggcacg aatcaaactg
      721 gagattcgtc cgcgcggctc cgacgcgacg gaaacacttc gcgtcgatcg cgtcatcaac
      781 tgcaccggac cgcaaccgga catccggaaa gtagcatcgc cgctcctgca gcgacttacc
      841 gaccgcggac tgatcgttcc tcatgaactc aacctgggta tcgaagcgac caccgacgga
      901 cggatcatcg acagtgacgg aaacgcgcaa accgattttc tgacgcttgg cacgctcctg
      961 cgcggcgccc tctgggaaag cactgccgtt cccgaactcc gtgtccaggc cgccggtgtg
     1021 gcccggcatc tggtgaactt gcttgccacg cgaacgacac cttaacaagc tttaacctct
     1081 gccggaaatc caaaccttcc ctcccgccgt ttgtttctaa aactgatatg aggatcctca
     1141 gcctttccat cttctccctg ctggtctccg cgctgatcgc gtgcggacaa ccggccgtcc
     1201 aacaacagca atcgaacaac atgcaagagc aaaacggaat ttcaaaagac gaactcaaaa
     1261 agaaactcac cccggaacag tatcgcatca cgcaggaatg cggcacggaa gcgcctttta
     1321 ccggcaagta ctgggacaac cacgagacgg gcatgtacca ctgcgtggtc tgtaatgctc
     1381 cgctcttttc aagcaacacg aaatacgaca gcggcagcgg atggccgagt ttctacgcgc
     1441 cggtcgaggg cagtaacatc cgcgaaaaac gggactcgag ccacggcatg atccgtaccg
     1501 agatcgtgtg cgcgaaatgc ggcgcccacc tgggacacgt cttcgacgac ggccccaacc
     1561 ctaccggact gcgctattgc actaactccg gttcgctgca gttctacaag gaaggcagta
     1621 agtaaatcat ccgatcaatg ctgcacccgc agtttcatgc tgcgggatgc agtttcggaa
     1681 acgagccgaa gcaggtaaag accggacgcc cattcggtcg tggagatgac catttgctcg
     1741 gcgctgacac gcgaccgata cagcacgcga cctgcgatgt ccgttacttc cagttgttcc
     1801 gtcccttttc cactcaggtg gttgaaactg acccgcactt cgtcacgtgc cggattcgga
     1861 aacgcgatga aggaaaccgc cggctcttcg agcgccggaa cgccggctgt cgggttgtac
     1921 tgataccaca gttgtagtcc gccggaagcc tgtcccagaa ccaggtcgac aaagccgtca
     1981 ccgttgatat caccaccgtt caccgaaaca ttgaacttga tcctggacga ttccggcttg
     2041 ttgtacagcg aatccaggag ccggaacgta ccgttcaggt taccgtcgat gttgtcgtag
     2101 aagtacacgt tgccgcccat gttcgacact gccagccgac gcgtgcctgc ggtatcgtaa
     2161 aagaagggta cggtataccc ttcgacgtat ccctgtgact gcagcagaat accgccaagc
     2221 gtatcgttat cgggcaggtt gctgtaaaac gcgctcgccg cagttccgat gttcttgaag
     2281 tagttgatgg tggccgactt ctccccgatc aacagatcct gcagtccgtc ccggttgatg
     2341 tcaaacagtt gcggggtcgc cgtgttaccg acatcgatgc ccatgtaatt cgggaatgca
     2401 agttggaagc tgcagggatt acccgcgccg gcgctgttgt tgaagaagta gagttttccg
     2461 tcgtccgcac cgatgatcat gtcggcatcg ccatcggcat ccagatcacc gaacgtcgcg
     2521 gccaggggcg cgacgagatt cagtgccgag atgccggcca ggtcgtccgt gatgagttcg
     2581 tatgccggct gggtggctgt gcctttgttg cggaacaacc acaagctgtt cttatttgcc
     2641 tgcggtggat tctggaagat actgcccccg acgatcaggt ccaacaagcc atcactatcg
     2701 taatcgaatg gcaccggcac agccgcttcc ccgacgtcga tcatgccgcc gaccagaaaa
     2761 tcactcgaac ggtaactgaa ggtcgggttg ggatccgttg cggtgttctt gtaccaccac
     2821 ataccctgtc ggttctcgtt ctcattcggc gcgaagagca tatcccgctt gccgtcgttg
     2881 tcgaggtcga tgtagctgtg cgcgtggaag ccgttgaaga ccgcggccag gtcgtaggat
     2941 ggaaagctga catcctgcgt atccatctcc gcggaaaact gcgtcccgcc gttgtggacc
     3001 atgagagtgt tgtacgaaga gatgtctccg atcagggcat ccatatcgtt gtcgccgtcg
     3061 aggtcgagca ggtagatcga agagatcgta tcatcacgac gagcg
//
LOCUS       DAOTON010000270         3096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24704_length_3096_cov_3.098323, whole genome shotgun sequence.
ACCESSION   DAOTON010000270 DAOTON010000000
VERSION     DAOTON010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24704_length_3096_cov_3.098323"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="P5292_06310"
     CDS             <1..943
                     /locus_tag="P5292_06310"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HRS38775.1"
                     /translation="HLPDESENYTHRSGRTARAGRSGLSLALVNVRELDKIRQIEKKI
                     NAKFHLSRIPDALEIGEQQLINFIRKVHEVEVDERGVEKFTEIAMHELADLSKEDVLK
                     RIISMEFNRFIDYYRNAPDLNVDLAHQGKQRQDRYRSNGPKVFINLGTVDGFDKGRML
                     GYVCDITGLKGSDIGRIDLKGVYSFIEVTDEKMIAVFEQSFQGEFHRGRPVRIERTGG
                     EESGGGKRNSSDFKGKGKPAFSKPSYEQDRGKKKGYPKKEYGGGKSGFAEKKTGKNAK
                     QDNYAPKKEGFSIPERKYSGYGGKKSVNKNNKKQGRW"
     gene            996..2060
                     /locus_tag="P5292_06315"
     CDS             996..2060
                     /locus_tag="P5292_06315"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRS38776.1"
                     /translation="MSTLKFSGNHSAYYLELKSKVEAYFAEHKIEMHGDSRNVIKSIV
                     LLSAFLTCYILLVFAIVPGWWAAPICVLFGIVTAGIGFNIMHDGGHGSLSANPTLNKL
                     AALSLNLLGGSSFFWNIKHNVIHHTYTNIDDHDDDILIEPFFRMTKAQKKIGIHRWQY
                     LYWPLAYGTMYLAWVFILDFKKYFSRQIGQRDNIKLPLRMHIGFWLTKILYVLFYVVL
                     PLQFHSITAFLIGFGIYTFTTGLIISVVFQLAHAVEEVNFIIPAADQHHLDTDWATHQ
                     LRTTANFATGNRIVSWFTGGLNHQVEHHLFPRISHVHYGAISPIVREVSTRHGLPYYE
                     APSVRHAILSHVRFLRRMGR"
     gene            complement(2088..>3096)
                     /locus_tag="P5292_06320"
     CDS             complement(2088..>3096)
                     /locus_tag="P5292_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38777.1"
                     /translation="LIMKLRVTVSLLTRLVIFLLLLPPSTMSAQNAVYNEWKRKAEQL
                     YMTGDFLASARAYDQAFAALDGKGLLDDRYNAACSWSLAGRPDSAFYHLFRLAEKANY
                     TNLGHLTTDPDLQPIHQDERWQQLISRVEANKKAAEAKLDPVLAPMLDSVFQQDQAGR
                     QMIAEVERRYGAGSKQMDSLWRSIQFHDSVNQEKVIAVLETRGWLGSDLVGAEGSQAL
                     FLVIQHADLPVQEKYLPMMREAVKAGKASGSSLALLEDRVALRKGGRQRYGSQIGLDP
                     ETGEKYLSPLEDPDQVDQRRAEVGLGPLSDYLLNFGLVWDVEEYKKQLPALEKRLGTI
                     K"
BASE COUNT          728 a          874 c          809 g          685 t
ORIGIN      
        1 ccacctgccc gacgaatcgg aaaactatac ccaccgcagc ggccgtaccg cacgggccgg
       61 tcgatcgggt ctttcccttg ccttggtgaa cgtacgtgag ctggacaaga tccggcagat
      121 cgaaaagaag atcaacgcaa aattccacct gtcacgcatt ccggacgcgc tggagatcgg
      181 cgaacagcaa ctgatcaact tcatccgtaa agttcacgag gtggaagtgg atgaacgcgg
      241 tgtggaaaaa ttcaccgaga tcgcgatgca tgaactggcg gatctcagca aagaggacgt
      301 actgaagcgc atcatcagta tggagttcaa ccgattcatc gactactacc ggaacgctcc
      361 cgatctgaac gtcgacctgg cgcatcaggg gaagcagcga caagaccggt accgttcgaa
      421 cggcccgaaa gtgttcatca acctcggcac cgtcgatgga ttcgacaaag gccgcatgtt
      481 gggatacgtc tgcgacatca cggggctgaa aggttccgac atcggccgca tcgacctgaa
      541 aggggtttat tccttcatcg aagtgaccga tgaaaagatg atcgctgtgt tcgaacaatc
      601 tttccaggga gaattccatc gtggccgacc ggttcggatc gaacgcaccg gaggcgaaga
      661 gagcggaggc gggaaaagaa attcgagcga tttcaagggc aagggtaagc cggcattttc
      721 gaaaccttcc tacgaacagg atcgtggtaa gaaaaaaggt tatccgaaaa aggaatacgg
      781 cggaggaaag tcgggcttcg cggaaaagaa gaccggcaaa aacgccaaac aggataatta
      841 cgcccccaaa aaagaggggt tctccatccc ggagcgcaag tattcaggct atggcggcaa
      901 gaaatctgtg aataagaaca ataaaaaaca gggccgctgg taaatagccg aataatccgg
      961 tcgcttatct tagcagcagc ctgtaataca tctgcatgtc tacattgaaa ttcagcggta
     1021 atcattccgc ctactacctc gaactcaaga gtaaagtcga agcctacttt gccgagcaca
     1081 aaatcgaaat gcacggcgat tcgcgcaacg tgatcaagtc gatcgtgttg ctttcggcat
     1141 tcctgacctg ctacattctg cttgtcttcg cgattgttcc cggctggtgg gcggcgccga
     1201 tctgtgtcct tttcggcatc gttactgccg gcatcggctt caacatcatg cacgacggcg
     1261 gtcacggtag cctctcggcc aatccgaccc tgaacaagct tgcagccttg tcgctcaacc
     1321 tgctcggcgg aagttccttt ttctggaaca tcaaacacaa cgtcatccac cacacctaca
     1381 cgaacatcga cgaccacgac gacgatattc tgatcgagcc attcttccgg atgacgaagg
     1441 cgcaaaagaa gatcggcatc catcgttggc agtacctcta ttggccgctg gcctacggca
     1501 cgatgtatct ggcctgggtt ttcatcctcg acttcaagaa gtatttttca cgccagatcg
     1561 gccagcgcga caacatcaag ctgccgttgc gtatgcacat cggattctgg ctgaccaaga
     1621 tcctgtatgt actgttctat gtcgtgctgc cgctgcagtt ccacagtatc accgctttcc
     1681 tcatcggatt cgggatctac accttcacca ccggcttgat catcagtgtc gtttttcagt
     1741 tggctcatgc ggtggaagaa gtcaacttca tcatcccggc ggctgatcag catcacctgg
     1801 acaccgactg ggctacgcac cagttgcgca ccacggccaa cttcgcgacc ggtaaccgta
     1861 tcgtttcctg gttcaccggc ggactcaacc accaggtgga acaccatcta ttcccacgga
     1921 tctcccacgt gcattatgga gccatcagtc cgatcgtacg cgaagtgagt acgcggcatg
     1981 gattaccgta ctacgaagct ccgagtgtcc gacacgcgat cctatcgcac gtgcggtttc
     2041 tgcgacggat gggacgataa gtccgatcca gtcagacagg ctgccattca ttttatcgtt
     2101 ccgagtcttt tctccaaagc gggcaactgc ttcttatact cttccacgtc ccataccaga
     2161 ccgaagttca acaggtagtc ggagagtggg ccaagtccca cctcggctct gcgctgatcg
     2221 acctgatcgg gatcttccag cggagacagg tatttctcgc ccgtctccgg atcaagccct
     2281 atctggctac catagcgttg tcggccacct ttccgcagtg caacccggtc ttccagcaat
     2341 gccaggcttg atccgcttgc tttcccggcc tttacggctt cgcgcatcat gggcaggtac
     2401 ttttcctgta ccggaaggtc ggcatgttga atgaccagga agagcgcctg acttccttcg
     2461 gcgccaacca ggtcggagcc cagccagccg cgcgtctcca gcaccgcaat cactttctcc
     2521 tgattcaccg aatcgtggaa ttggatgctc ctccatagcg agtccatttg tttggagccg
     2581 gcgccgtatc tgcgttccac ctccgcgatc atttgacgac cggcctggtc ctgctgaaag
     2641 acggaatcga gcatgggcgc caggaccgga tcgagttttg cttctgcggc ttttttattc
     2701 gcctcgacac ggctgatcaa ttgctgccat cgttcatcct gatggatggg ctgcaggtcc
     2761 ggatccgtcg tgagatgccc caggttggta tagttcgctt tttccgccag tcgaaagagg
     2821 tgatagaatg cggagtccgg tcgccccgcc agtgaccagc tacaggctgc attgtagcga
     2881 tcatcgagga gtcctttccc atcgagtgcg gcaaatgcct gatcgtaggc gcgtgcggaa
     2941 gccagaaaat ctccggtcat atacaattgc tccgcttttc gcttccactc gttgtacacc
     3001 gcattttgtg cagacatggt ggacggtggg agcaggagta aaaagatgac caggcgggtc
     3061 agcaaggaaa cggttacacg cagtttcatg atcaaa
//
LOCUS       DAOTON010000271         3095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24713_length_3095_cov_4.846053, whole genome shotgun sequence.
ACCESSION   DAOTON010000271 DAOTON010000000
VERSION     DAOTON010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24713_length_3095_cov_4.846053"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            396..1019
                     /locus_tag="P5292_06325"
     CDS             396..1019
                     /locus_tag="P5292_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRS38778.1"
                     /translation="MKYPASINHKTNSGRRALQKSQMGHAYKIHKQDGVYFLTLTIVD
                     WVDLFTRSSYKEIITNSLNYCVEEKGMEIYAYVLMTNHLHIIAAAKNNNLSDIIRDFK
                     RYTSQKIIEQISSNRESRRNWLLPIFRSAASKHKRNTCYQIWTHDNHAEEVYSPAFTK
                     SKINYIHKNPVKAGMVGRPEEYLFSSARDYAGDQGPVKVLKIELQLL"
     gene            1297..2247
                     /locus_tag="P5292_06330"
     CDS             1297..2247
                     /locus_tag="P5292_06330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRS38779.1"
                     /translation="MKVRLLLLLLLAPLLYSSVRQPDWGDDFAQYLLQTRNIVEGRSM
                     QDNGIVADPALPAFAIPAYPVGFPLLLLPFYSSDPFRTAPYFIVPALSLLLAAFACFL
                     LLRKRFGVTTSLLSALLIAYHPVFLQAKTELLSDLPFIAFLLLGLQAMATEKEEPISR
                     RNIVFAGLSFGLAMSMRLTGFLLLPTLLAWSVFDRSKRSAAGKIAGIALLLFAILNVV
                     LFPIDLPGIWQFYRGAIAAQPLLLTDQFIFYLHQLNALFWISPFSWTGGLIVPGMLVY
                     YWRKRDSLDAADWFLLAYAGLLMTIPYQGGGLRFLLPAIP"
     gene            2251..2718
                     /locus_tag="P5292_06335"
     CDS             2251..2718
                     /locus_tag="P5292_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38780.1"
                     /translation="MRNLLSLVRPFVLPVLQFRRYLHVAYAGIVLVLILPGIVDWLTT
                     LERNDGPESLEGKRILSIVEQKTPKDAVIICAKPRAIQWYTGRRATYLIDGVLPARID
                     SAFAQLNARYLICERTGRPGAFADDKMNAYLLSFGDRYVPVDSTKQLTLWLRK"
     gene            2784..>3095
                     /locus_tag="P5292_06340"
     CDS             2784..>3095
                     /locus_tag="P5292_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38781.1"
                     /translation="MIPKILRILRNRPQLAETVFIPLFWALYFVYLLFRGKQFILAGF
                     PDRPISIASFDGVDVTERVRTFYYAIGLSLLLFVGLLFAIQKLLHRLRNGELRVLQAS
                     AV"
BASE COUNT          678 a          799 c          818 g          800 t
ORIGIN      
        1 aaaccggtgt tgggcgtagt ttgaagggga ggattgcggg caggaactac gcctttgttt
       61 cgacgaagtt gctttcataa cggcgagcag gtcgtagttc cggccgcaca gtgccctcgc
      121 gccaaactac gaccatcaac ggcagtgggc agttggcggt tggcagtagg cggttggcag
      181 tgggacaagg acgtacattc tgcctactgc ctactgaaaa ctgaaaactg gctgttggca
      241 gtgggacgaa ggacgtgcct actgcctact gcctaccgct gactgctgac tgaaaaaccg
      301 gtgttgggcg tagtttgaag gggaggattg cgggcaggaa ctacgccttc atttcgacga
      361 tcaatttgcg atttcattat tatatccttt gatcattgaa atatccagca tctattaatc
      421 ataaaacaaa ttcaggtcgt cgagctttgc aaaaaagcca gatgggacac gcatataaaa
      481 ttcataaaca agacggcgtt tactttctca ccttgacgat cgtcgattgg gtggatctat
      541 ttaccagatc cagctacaag gaaattataa cgaatagctt gaactattgc gtggaagaaa
      601 aaggtatgga aatttatgcc tatgtgttaa tgaccaatca ccttcacata attgcggcag
      661 caaaaaacaa taatcttagc gatataatcc gtgattttaa gcgctatacc agtcaaaaaa
      721 tcatcgagca gataagctca aaccgggaaa gcagacgaaa ctggttgctt ccaattttcc
      781 gaagtgccgc atcaaagcat aagcgaaata cctgctatca gatttggacg catgacaatc
      841 atgcggaaga ggtctattct ccagcattca cgaaaagcaa aataaactat attcataaaa
      901 atcccgtcaa agccggcatg gttggcagac ctgaggaata tctattcagc agcgccagag
      961 actatgccgg agatcaagga ccggtgaagg ttttgaagat tgaactgcaa ttgctttaat
     1021 ccggcgagca ggtcgtagtt ccggccgcac agtgccttcg cgccaaacta cgaccatcaa
     1081 cggcagtggg cagttggcgg ttggcagtgg gcggttggca gtgggaagaa ggacgagcat
     1141 tgcatactgc ataccgcata ttgcctactg aaaactggca gttcgtagtt ccggccgcac
     1201 agtgccttcg cgccaaacta cgaccaacaa ctaagaatgc gggttggaac tacacccccg
     1261 cccttagtac attcgcttcg ctcacaggta tccattttga aggtccggct tcttttgttg
     1321 ttgctcttag cgccactgct gtactccagt gtgcggcagc cggactgggg tgatgatttc
     1381 gcgcagtacc tgttacagac gcggaacatc gtggaaggac ggtcgatgca ggacaatggc
     1441 attgtcgccg accccgcttt gccggccttt gccataccgg cttatccggt gggtttcccg
     1501 ttgttgttgc ttcccttcta ctcttccgat ccattccgta cagcgcctta tttcattgtg
     1561 ccggctttgt cgttgctgtt agccgccttc gcctgcttcc tgttgttacg caagcgattc
     1621 ggtgtcacca cctctttgct gagcgcatta ctcatcgcgt atcatccggt attcctgcag
     1681 gcgaaaacgg agttgttgtc ggacctgccc tttatcgcct tcctgctgtt gggcctgcaa
     1741 gcgatggcga ccgaaaaaga agaaccgatt tcccggcgaa acatcgtatt cgccggcttg
     1801 tcgtttggcc ttgccatgag tatgcgactg acgggatttc tgttgctgcc gaccttgttg
     1861 gcctggtcgg tctttgaccg ttcgaagcgg tcggcagccg gcaagatcgc aggcattgcg
     1921 ttgctgttgt ttgcgatcct aaacgtagtg ttgtttccga tcgaccttcc gggaatctgg
     1981 cagttctatc gcggcgccat tgccgcgcaa ccgttgttgc tgacggatca gttcattttc
     2041 tatctgcatc agctcaatgc cttgttctgg atcagtccgt tctcctggac cggcgggctg
     2101 atcgttccgg gcatgttggt gtactactgg cgcaaacgag attcgcttga tgcggccgac
     2161 tggtttctac tggcgtatgc cggcttgctg atgaccatac cttatcaggg tggcggtttg
     2221 cgcttcctct tgcctgccat accatagttg ttgcgcaatc tgctcagtct tgtgcgaccg
     2281 ttcgtactcc cggtgctgca attccggcgt tacctgcacg tagcgtatgc cggcatcgtg
     2341 ttggtcctga tcctgccggg catcgtggat tggttgacaa cccttgaacg aaatgacggt
     2401 cccgagtcac tcgagggaaa acgtatcctg tcgatcgtag aacaaaaaac accgaaagac
     2461 gcggtgatca tttgtgccaa gccgcgggcg atccaatggt acaccggacg ccgggccacg
     2521 tacctcatcg acggcgtact gccagctcgg atcgacagcg cttttgctca attgaatgcc
     2581 cgttatctga tctgtgaacg taccggcagg cccggcgcat tcgcagacga taaaatgaat
     2641 gcctacctcc tttcgttcgg ggaccgctat gtgccggtcg acagcacgaa acagttgaca
     2701 ctctggttgc gtaagtgagg ctccggacgt ggattcggct tccggctttt cgctacattt
     2761 acccgtaacg cgcgcccaga caattgattc ctaagatctt acgcattctt cgcaatcgtc
     2821 cgcagcttgc cgaaacggtt ttcattccgc ttttctgggc attgtatttc gtttacctgc
     2881 tctttcgcgg gaagcaattc atactggccg gatttccgga ccgacccatt tccattgcga
     2941 gcttcgacgg ggttgatgtg acggagcgcg tgcgtacgtt ctattacgcg atcggcttat
     3001 cgctgttgct gttcgttggc ctgctgttcg ccatacaaaa gctgctacac cgcttacgaa
     3061 acggggagtt gcgggtcttg caggcgagtg ccgtc
//
LOCUS       DAOTON010000272         3092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24744_length_3092_cov_4.033257, whole genome shotgun sequence.
ACCESSION   DAOTON010000272 DAOTON010000000
VERSION     DAOTON010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24744_length_3092_cov_4.033257"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="P5292_06345"
     CDS             <1..263
                     /locus_tag="P5292_06345"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRS38782.1"
                     /translation="YAGFLQGRVVDMLYFPLVSGRFPDWLPIWGGETFLFFRPVFNIA
                     DASISIGVILYILFQKRFHEGDHAQKTETDAPAATDASSGLS"
     gene            308..1690
                     /locus_tag="P5292_06350"
     CDS             308..1690
                     /locus_tag="P5292_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRS38783.1"
                     /translation="MKRSISLLVLSLFFTTSLQAQARSDSATVRSIYSEMLSNGKAYD
                     WLRVLSNDIGGRLSGSPEAQQAVEYTRKAMLEAGADTVWLQEVWVPHWVRGAKESGRI
                     IARTGDSQEVPVCALGGSVATPKEGISGNVVEVHDFDELKALGREKIEGKIVFYNHPF
                     DPTFINTFEAYGEAGKYRWSGPSEAARYGAIASIVRSMTLAQDDFPHTGAMGYNDSLP
                     KIPCAAISTNGANLLSNILRADPGARFLLRMDCQLLDSVLSHNVIGEIRGRELPDEVL
                     VVGGHLDSWDTGDGAHDYGAGVMQSVEILRTFKALKLRPKRTIRAIAFMNEENGLRGG
                     RGYAAWASTNSREKHVMAIESDAGGFTPYGFGLDMPEDKKPSIRKYRELFRPYNVWNF
                     EEEHGGADISPLKPSLKVPLVGLVVDSQRYFDYHHAGSDTFDKVNKRELLLGAAAMGS
                     LLYLVSENGF"
     gene            1706..>3092
                     /gene="uvrC"
                     /locus_tag="P5292_06355"
     CDS             1706..>3092
                     /gene="uvrC"
                     /locus_tag="P5292_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRS38784.1"
                     /translation="MASEEHIQTLLSALPDSPGVYQFYDKEDKLLYVGKAKSLKKRVS
                     SYFQKDRHVSGKTSIMVRKVADIRTIVVDTEMDALLLENNLIKKHQPRYNVSLKDDKT
                     YPWICIRNERFPRVFSTRKMIRDGSEYFGPYASGRLINTLLELINKLYKLRTCSLVLS
                     EENITKKKFRVCLEYQIGNCKGPCEGFQSQGEYDQSVKEIRQILKGNIQTVIGHLREL
                     MQTYAAEYRFEEAQSVKEKLESLEKFQRKSVVVNPSIHNVDVFSLVIEEDSAYVNFLR
                     VMNGAIIQGHTVELKKKLEETPEELLELAMGELRSRFFSDAEEIIVPFAPEVEWPGIS
                     FTIPKIGDKKHLLSLSESNVRNYIREKRLQEEKQHPENRTLRLMEQMKKDLRLTELPK
                     HIECFDNSNFQGAYPVAAMSVFRDGKPSKKDYRHFNIKTVEGPDDFASMEEVIHRRYK
                     RMLDEALPLSEF"
BASE COUNT          693 a          854 c          895 g          650 t
ORIGIN      
        1 ggtatgccgg cttcctgcaa gggcgggtgg tggacatgct gtatttcccg ctggtctcgg
       61 gacgctttcc ggattggtta ccgatctggg gcggtgagac gttcctgttc ttccgaccgg
      121 tgttcaacat cgccgacgct tcgatctcga tcggcgtgat cctgtacatc ctgttccaga
      181 agcgcttcca cgaaggcgat cacgcgcaga agacggagac cgatgctccg gccgccactg
      241 acgcctcctc cggactttcc tgattttccc gatccttttt ttcggattca gtatcttgtt
      301 gcttcctatg aagcgatcga tttccctcct ggtactgtcc ctctttttca cgacttccct
      361 gcaagcacaa gctcggagcg acagcgcgac cgtccggtcc atctactccg aaatgctctc
      421 gaacggcaag gcctacgact ggctgcgcgt gttgtcgaat gatatcggtg gacgacttag
      481 cggatcgccg gaggcgcaac aggccgttga atacacccgg aaggccatgc tcgaagccgg
      541 cgccgatacg gtttggttgc aggaggtatg ggtgccgcac tgggtgcggg gcgctaaaga
      601 aagcggacgc atcatcgcgc gtacaggcga ttcgcaggaa gttccggtct gcgcactcgg
      661 tggctccgtc gcgacaccga aagaagggat cagcggcaat gtcgtggagg tgcacgactt
      721 cgacgaactc aaagccctgg gacgcgaaaa gatcgaaggc aagatcgtct tctacaacca
      781 cccctttgat cccaccttca tcaatacgtt tgaagcatac ggcgaagcgg gcaagtaccg
      841 ttggtcgggt ccgagtgaag cggcccgtta cggtgccatc gcttccatcg ttcgctccat
      901 gaccctggca caagatgatt tcccgcatac cggagccatg ggttataatg actcattgcc
      961 gaagatcccc tgtgccgcca tcagtacgaa tggcgccaac ctgctcagca atatccttcg
     1021 cgcagatccg ggtgctcgat tcttgctgcg catggactgc cagctcctcg acagtgtact
     1081 ttctcacaac gtcatcgggg agatccgcgg tcgcgagctg ccggatgaag tgctggtcgt
     1141 cggtggtcac ctcgattcct gggataccgg cgacggcgcg cacgattatg gcgccggcgt
     1201 gatgcaatcc gtcgagatcc tgcgtacgtt caaggccttg aagcttcgac cgaagcgcac
     1261 gatccgcgcg atcgcgttca tgaacgaaga gaacggtctt cgtggcggta gaggttacgc
     1321 ggcctgggca tcgaccaaca gccgggaaaa gcatgtgatg gccatcgaaa gcgatgcagg
     1381 cggattcacc ccgtatggat tcggactgga tatgcccgaa gacaaaaaac cttccatccg
     1441 gaagtaccgt gagttgtttc gtccttacaa tgtctggaac ttcgaagaag agcacggtgg
     1501 cgccgacatt tctcccctga agccttcgtt gaaagtaccc ttggtaggcc tggtggtcga
     1561 ctcccaacgc tacttcgatt atcatcatgc cggctccgac acgttcgaca aagtgaacaa
     1621 gcgcgaactc ttgctgggag ccgctgccat gggttccttg ctttacctcg tgagcgaaaa
     1681 cggtttctga ccgcgcgaag cgcacatggc ttccgaagaa cacatccaga ccctgttgtc
     1741 ggcgctaccc gactcgccgg gcgtttacca gttctacgac aaggaggaca agctgctcta
     1801 tgtgggcaag gccaagtcgt tgaagaagcg ggtcagcagt tatttccaaa aggaccggca
     1861 cgtctcgggt aagacgtcca tcatggtgcg caaggtggcc gacatccgca ccatcgtcgt
     1921 ggatacggag atggacgcgc tcttgctgga gaacaacctc atcaagaagc accagccgcg
     1981 gtacaacgtc agtctgaagg acgacaagac ctatccgtgg atctgcatcc gcaacgagcg
     2041 tttcccgcgc gtcttttcga ctcgcaagat gatccgtgac ggttccgaat atttcggacc
     2101 gtatgcttcc ggccgtttga tcaatacgtt gctggagctg atcaacaagt tgtacaagct
     2161 gcggacgtgc agcctggtgt tgtcggagga gaatatcacg aagaagaaat tccgtgtctg
     2221 cctcgaatac cagatcggta attgcaaggg accttgtgaa ggatttcaat cacagggaga
     2281 gtacgatcaa tcggtgaaag agatccggca gatcctcaaa ggaaacatcc agacggtcat
     2341 cggtcacctg cgcgaattga tgcagaccta cgcggccgag tatcgctttg aagaagcaca
     2401 atcggtcaaa gagaaactcg aatcactcga aaagttccag cggaaatcgg tggtcgtgaa
     2461 tccgtcgatt cacaacgtgg atgtgttctc cctcgtgatc gaggaggatt ccgcgtatgt
     2521 gaatttcctg cgtgttatga acggcgcgat catccagggg catacggtgg aattgaaaaa
     2581 gaagctggaa gaaacgccgg aggagttgtt ggagcttgcc atgggcgagt tgcgctcgcg
     2641 cttcttcagc gacgcggagg agatcatcgt acccttcgcg ccggaagtcg aatggcccgg
     2701 catttcgttc accatcccga agatcggcga caagaagcac ttgctcagcc tgtcggagag
     2761 caacgtgcgg aattacatcc gcgaaaagcg gttgcaggaa gaaaagcagc atcccgagaa
     2821 ccgcacgcta cgcctgatgg agcagatgaa aaaagacctg cggctcaccg agttgccgaa
     2881 gcacatcgag tgtttcgaca attccaattt ccagggcgcc tatcccgtag cggcgatgtc
     2941 ggtcttccgg gacggcaagc cgtcgaagaa ggactaccgg catttcaaca tcaagaccgt
     3001 cgagggtccc gacgatttcg cgtcgatgga ggaggtgatc caccggcggt ataagcggat
     3061 gctcgacgag gcgctgccgc tgtcggagtt ca
//
LOCUS       DAOTON010000273         3088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24788_length_3088_cov_2.575668, whole genome shotgun sequence.
ACCESSION   DAOTON010000273 DAOTON010000000
VERSION     DAOTON010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24788_length_3088_cov_2.575668"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            497..1255
                     /locus_tag="P5292_06360"
     CDS             497..1255
                     /locus_tag="P5292_06360"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014095688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRS38785.1"
                     /translation="MNQLSNQTAIITGAASGMGKAMAILFAAEGARVVAADLHQAELD
                     TVVESIRQSGGTAIGIPCNVSLESDIRSLIAGAMEHFGSVDILVNNAGVMDDFHPVEH
                     VSNDVWNKVLRINLDGPFYACRAAIPIMLKQGKGVILNIASVGGLHGARAGAAYTTSK
                     HAVIGLTKNIGYVYAQKGIRCNAIAPGGVNTNIGRDMHPDPFGYERSLPGMGTMPRMG
                     EPEEIARTALFLVSDASSFVNGAVLTADGGWTAY"
     gene            complement(1277..1894)
                     /locus_tag="P5292_06365"
     CDS             complement(1277..1894)
                     /locus_tag="P5292_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRS38786.1"
                     /translation="MNIAIIGSGNVGGALASRWAAKGHRIFLGVRDPQHFKGQALTEL
                     PAVAVFTIPEAVRQSEAILLATPATAAIEVAQSLGDTAGKVILDAMNVVMGRGPQGFS
                     NTADAILAHTASRDVVKCFNTTGFNNMKDPIYQGMPIDLFMAGDSARGKAIARQLAID
                     AGFAECYDAGGNDKFQLMEQFAFFWINLAMMQGLGREIGFKLLRR"
     gene            complement(2013..>3088)
                     /locus_tag="P5292_06370"
     CDS             complement(2013..>3088)
                     /locus_tag="P5292_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRS38787.1"
                     /translation="IGPWNFPFLLMVGPLISALAAGNATILKPSEFAPATGRLLTQMV
                     AAVFPADLVCVKKGDHTVSEQLLTLPFDHVFFTGSARVGKIVHAAAAQLNASVTLELG
                     GQCPVIVCADANLEDAARKIVAAKFFNCGQACVAPDHVLVDARIFDPFLNILREQVTA
                     LFGDSQQIEQSPDYGRLIHRQHAERLHKLLNDALASGAVLRLGGISRPEQRFMAPTII
                     TGTASSMRIRQEEIFGPILPVEPFESLETALLESGGQDIPLAVYAFTRSRTNLELIRD
                     ERRSGALLWNDCAVHFLHPGLPFGGLQGSGLGRAHGHAGFLTFSNQRSILKQRIGWTG
                     IRLFYPPYSRLKRRLADLLLRWF"
BASE COUNT          748 a          798 c          859 g          683 t
ORIGIN      
        1 ccaggtcagg actcccgcca tcaacatcat cgggatgagc gcgacgagga atgaacgcat
       61 gatcaatgat cgcttctgat ggttcaactc caattcaagc gaaccttcta aaacgatcat
      121 gatcagaccg atggttccaa taaccggtag taagggagat aattcaggta actggatatg
      181 caaccacgaa acgaggtggc ggacgagcca accagtcaac agtaagagta tgactgaagg
      241 gatccgagac cgtcctgcgg ttagatcaaa gatgtaagcc agaagaaaca ggcagcaaaa
      301 cgtaatcagg atcgcagagg tcatacaaat atcccttcag ttttttcccc acaaaatgtg
      361 ctagtcatct ttccgtaaat ttagatcatt gggggatttt ctgatagtcg tcatggtggg
      421 aataatgaaa tagttgtcac tttgtagttg aattgtacgt gacaagtctc aaacttgaaa
      481 ttaaaccaca aatcgtatga atcaattgtc caatcaaacc gcgatcatta ccggagccgc
      541 ttccggaatg ggtaaggcca tggccatttt attcgctgca gaaggtgcca gggtggtggc
      601 agcggatctt catcaggcgg aactggacac ggtagtcgag tccattcgcc aatccggtgg
      661 tacggctatc ggcattccgt gtaatgtcag cctggagtcg gatatccggt ccctgattgc
      721 gggagcgatg gagcatttcg gtagtgtcga cattctggtg aacaatgccg gcgtcatgga
      781 tgactttcac ccggtcgagc atgtttcaaa tgatgtctgg aacaaggtgc tccgcattaa
      841 cctggacgga cccttttacg cttgccgcgc agcgataccg atcatgttaa aacaaggtaa
      901 aggggtgatc ctgaatatcg cgtccgtcgg cgggctccac ggagcgcggg ccggagctgc
      961 ttatactact tccaagcacg ctgtgatcgg tctgacgaag aacattggat acgtctatgc
     1021 ccaaaaggga atacgttgca acgcgatcgc gcccggtggt gtgaatacca acatcggccg
     1081 cgatatgcat ccggatccgt tcggctatga gcgcagcctc ccgggcatgg gtacgatgcc
     1141 ccggatgggc gagccggagg agatcgcccg aacggcactg ttcctggtct ccgacgcttc
     1201 cagttttgtc aatggagctg tgttgaccgc cgatggcggg tggacggcct actgatccga
     1261 ttcagggaaa gctacttcaa cgcctgagga gcttgaatcc gatttccctg ccgagtccct
     1321 gcatcatcgc gagattgatc caaaaaaagg cgaattgctc catcagttgg aacttatcgt
     1381 tgccacctgc atcatagcat tccgcgaatc cggcgtcgat tgccagttga cgagcaatcg
     1441 cttttccccg ggcgctgtcg cccgccatga agagatcgat cggcatgcct tggtagatgg
     1501 ggtctttcat gttgttaaaa ccggtcgtat tgaaacactt caccacatcc cggctggcag
     1561 tatgagccag gatggcgtca gccgtattcg aaaatccttg cggaccgcgc cccatcacga
     1621 cgttcatcgc atcaaggatg accttgccgg ctgtgtcgcc cagcgattgt gccacctcta
     1681 ttgcggcggt agcgggtgtc gccagtagga ttgcctcgct ctgacggact gcctccggaa
     1741 tggtaaagac ggctacggca ggcaattcgg tcagcgcctg gcctttgaaa tgctgtggat
     1801 cgcgtacacc aaggaagatg cgatgtcctt ttgcggccca acgggaagcc agggctccgc
     1861 ctacgtttcc gctaccaata atggcaatgt tcatggattg actgagttgg aagatgacaa
     1921 agttggcaag gataacattt ccttcatatt actatcttaa tagaaagtaa tagtcaaaaa
     1981 ggatagtaac aggcacctgc aacctgtttt tctcagaacc agcgaagcaa caagtcggca
     2041 agccgacgct tgaggcgcga gtagggcgga tagaacaagc gaataccggt ccagccgatc
     2101 cgctgtttca aaatggagcg ctggttgctg aacgtcaaaa agccggcatg tccgtgtgcg
     2161 cgaccgagtc cgcttccctg aagaccaccg aagggtaatc cgggatgcag gaagtgaacg
     2221 gcgcaatcgt tccacaacaa agctcccgac ctccgttcat cccggatcaa ttcaaggttg
     2281 gtgcggctgc gggtgaacgc atagactgca agcggaatgt cctgacctcc cgattccaac
     2341 aaagccgttt ccagcgattc gaatggttcc accggcagta tcggcccgaa aatttcttcc
     2401 tgccggatgc gcatgcttga cgctgttccg gtgatgatgg tcggagccat gaatcgctgt
     2461 tccggacgtg atattccgcc gagtcgaagc accgcaccgg acgcgagggc gtcattcagg
     2521 agtttatgca ggcgttcggc atgttgccgg tggatgagtc ggccataatc cggggattgt
     2581 tcgatctgct gggaatcgcc aaagagcgcc gtcacctgtt cacgcaggat attcaggaat
     2641 ggatcgaaga tccttgcatc caccagaacg tggtccgggg caacacaggc ttgcccgcag
     2701 ttgaaaaatt tggcagcgac gatctttctt gccgcatctt ccaggttggc atccgcgcaa
     2761 acgatcaccg gacactgccc gccgagttcc agggttacgg atgcatttaa ctgagctgct
     2821 gcagcgtgca cgatcttgcc cacccgggcg cttccggtaa agaatacatg atcgaacggc
     2881 agggtcaata actgctcgga gacggtatga tcacctttct ttacacaaac aagatcggca
     2941 gggaagacgg ccgcgaccat ttgtgtcagc agtcgtccgg tagcgggggc gaactcggaa
     3001 ggtttcagta tggttgcatt gccggctgcc agagcactga taagcggtcc gaccatcaat
     3061 aggaatggga agttccacgg gccgatca
//
LOCUS       DAOTON010000274         3086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24809_length_3086_cov_2.900363, whole genome shotgun sequence.
ACCESSION   DAOTON010000274 DAOTON010000000
VERSION     DAOTON010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24809_length_3086_cov_2.900363"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="P5292_06375"
     CDS             complement(<1..432)
                     /locus_tag="P5292_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38788.1"
                     /translation="MLLPDYKPAHGYGIRSLFLLLFLLLGGCFSPGELANRNMAGNYR
                     PMDGQSLSAEVTIRQVNDSLARVLIRIKPDEYLYVRQEDNRYLATVGIHLQLVESYDV
                     PTRYDSAVARYTMDMTEKGKARIFEVELPVHRKGTILLETVI"
     gene            complement(419..1765)
                     /gene="rmuC"
                     /locus_tag="P5292_06380"
     CDS             complement(419..1765)
                     /gene="rmuC"
                     /locus_tag="P5292_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRS38789.1"
                     /translation="MEFVYLLVGLVVGVLLGWLLGKAGVQSVREEQRTAVQALESERS
                     ALAERARQLEQQLQEGRSRQEQDRQQQLELNGKLSEAVTLNRNLQEKLQSQKAELEAL
                     QDRFTKEFENLANRILEEKSQKFVEQNRNNLDVILNPLKEKIRDFEQKVDQAYKSEAA
                     ERNSLKGEIGKLVELNKQISDEAHNLARALKGDTKKQGNWGEFILEKILEHSGLERDR
                     EFKVQHSTSNEEGRRVQPDVVVFLPDNKHLIVDSKVSLVAYEAMVNAGSEEEREQYLR
                     EHVQSVKNHVRQLSEKKYQSAEGMNAPDFVLLFMPIEPSFSVAIQGDTELFAYAWDRK
                     IVIVSPSTLLATLRTIASLWKQERQTRNALEIARVGGALYDKFKGFIDDLIDVGKKMD
                     AAKAGYSDAMNKLSSGPGNIIKRIEDLRKLGAKSSKELPQALVERALESGDTDAAP"
     gene            1873..2970
                     /locus_tag="P5292_06385"
     CDS             1873..2970
                     /locus_tag="P5292_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38790.1"
                     /translation="MRIDRTYRWLIAFFLLTGSSSLRAQVPNNQLDLQATDWGLKGSV
                     YHIARVEDERKKAGDAGSISGGGPVQPIRFKQDLAKDLLPYVQTCTAADTSKVALVLA
                     FEKFQLKENRTGPRRNIQFEFQVRIYRDVDGQRVQLYQVGGTPSYVVHGPNPGIYDKL
                     IGGSLQQALSGFDRWAKENRNQPPLCKHVRIELQDDGAFRDTVSADTIRWSSAYRLKW
                     ADFKGQPEQQVVFSANSACVYSFTSRPAYRDDTLILELLLHPAFIKPASWVKPDKLQD
                     GLLAHEQLHFDICELYARRMRDRFTQSSPGLLTYDSVLKTIFESEWQAYQLRQADYDR
                     ETEHGLIPEQQANWEKSISEELNRLGSFASQ"
BASE COUNT          670 a          872 c          776 g          768 t
ORIGIN      
        1 gatcacggtt tccaggagga tggtaccctt ccggtgtacc ggcagttcca cttcgaaaat
       61 ccttgccttc cctttctccg tcatgtccat cgtataacgc gcgacggcgc tatcatagcg
      121 agtcggaacg tcgtagcttt cgaccagttg caagtggatg ccaaccgtgg cgaggtatcg
      181 attgtcctcc tgacgcacgt agaggtactc gtccggtttg attcggatca gtacacgtgc
      241 cagggaatca ttgacttgac ggatcgtcac ttcagccgat aaggattgcc cgtccattgg
      301 ccgataatta ccggccatgt tgcggttggc aagttcgccg ggactgaaac acccacccag
      361 cagtaagaac agcaggagga acaggcttcg tatcccgtac ccgtgtgcag gcttatagtc
      421 agggagcagc atctgtatca ccgctttcga gcgcacgctc taccagtgcc tgaggtaatt
      481 ccttactgga cttcgcgcca agtttacgca agtcttcaat tcgcttgatg atgttgcccg
      541 ggccggacga tagcttgttc atcgcgtcac tgtagccggc cttggcggca tccattttct
      601 taccaacgtc gatgaggtca tcgatgaagc ctttgaactt atcgtacaag gcgccgccga
      661 cgcgggcgat ctccagggcg tttcgtgtct gtcgctcctg cttccaaagc gaagcgatgg
      721 ttcgcagggt cgccagcagg gtactcggac tgacgatgac aatcttacgg tcccaggcgt
      781 acgcgaacaa ttcggtatca ccctgaatgg cgacgctgaa ggaaggttcg atcggcatga
      841 acagcagtac aaagtcgggg gcgttcatgc cttcggccga ctggtacttt ttctcggaaa
      901 gctgccgtac atgattcttg accgattgca catgctctcg cagatactgt tcccgctctt
      961 cctcgctgcc ggcattcacc atcgcttcgt aggcaaccag ggaaactttg ctgtcgacga
     1021 tgaggtgctt gttgtcgggt agaaaaacga ctacatcggg ttgaacccgc cgaccttcct
     1081 cattgctcgt gctgtgttgc actttgaatt cgcggtcgcg ttccaatccg gagtgttcca
     1141 gaattttctc caggatgaac tcgccccagt taccctgctt cttggtatca cccttcagtg
     1201 cacgagccag gttgtgcgcc tcgtcgctga tctgtttgtt aagttcaacc agtttaccga
     1261 tctccccttt cagactgttt cgttcagcag cttctgactt gtatgcctga tcgacctttt
     1321 gctcgaaatc ccggatcttt tctttgagcg gattgaggat cacgtccagg ttgttgcggt
     1381 tctgttcaac gaacttctgg ctcttctcct ccaggatcct gttcgccaga ttctcaaact
     1441 ccttggtaaa ccgatcctgc agcgcttcga gttccgcctt ttgggattgg agcttctcct
     1501 ggaggttgcg attgagggtc actgcttcgc tcagctttcc gttcaattcg agctgttgct
     1561 gtcggtcctg ctcctgcctg gaccttcctt cctgcaactg ctgctccagc tgacgtgccc
     1621 gttcagccaa ggccgaccgt tcactttcaa gggcttgtac cgcagtacgc tgttcctccc
     1681 gcaccgattg aaccccggct ttacccagca accagccgag aagtacaccg actaccaatc
     1741 ccaccaaaag ataaacgaat tccatccgtt agacgcttgt aagtggttaa agatagcgga
     1801 tttcgtgcat ttttcgttgt tctggtcgaa tggcggttaa tcgcgaactt tgcgcccctt
     1861 caaaggatcc aaatgcgaat tgaccgaacc tatcgatggc tgatcgcctt ctttctgctg
     1921 accggaagtt cttccctgcg cgcccaggtt ccaaacaatc aattggatct gcaggcaacc
     1981 gattggggat tgaaagggtc ggtgtaccac atagcccgcg tcgaagacga acgaaagaaa
     2041 gccggagatg ccggtagtat atccggcggc ggtcccgtcc aaccgattcg cttcaaacaa
     2101 gatctcgcga aagatctgct gccttatgtt caaacgtgta ctgccgcgga tacgagtaag
     2161 gtagcactgg ttctggcatt cgagaaattt cagttgaaag aaaaccgcac aggaccacga
     2221 cggaacatcc aattcgaatt tcaggtgcgc atctatcgcg atgtcgatgg ccagcgcgtt
     2281 caactctacc aggtaggcgg aaccccctcc tatgtggtac atgggcccaa tcccggaatc
     2341 tacgacaaac tgatcggcgg atccctgcaa caagccctat ccggattcga ccgctgggcg
     2401 aaagagaacc gaaaccagcc accgctgtgc aaacacgtac gcatcgaact acaggacgat
     2461 ggcgcttttc gggacacggt cagtgctgat acgattcgat ggagttccgc ctatcggctg
     2521 aagtgggccg acttcaaagg acagcccgag caacaggtcg tattttccgc gaacagtgcc
     2581 tgcgtctatt ccttcacatc gcggcccgcc tatcgggatg acacacttat tctggagctg
     2641 ctgctgcatc cggcttttat caagccagcc tcctgggtta aacccgacaa gttgcaggat
     2701 ggactgctcg cccacgaaca actgcatttc gatatctgcg aattgtacgc ccgccggatg
     2761 cgcgatcgct tcacccaatc cagtccgggt ctattgacct acgattccgt tctgaaaacc
     2821 atcttcgaat ccgaatggca ggcctatcaa cttcgacagg ccgactacga ccgggaaaca
     2881 gaacacggcc tgattcccga acaacaggcg aactgggaaa aatcgatctc cgaggaatta
     2941 aaccggctgg gatcctttgc cagtcagtga aattgacgga attagcgatg ttgacttaat
     3001 tattctttca cttttcgttc acatttaata ttgagttaac acagccccgg gttggttggg
     3061 tttaatttgc gaccgatagc caacct
//
LOCUS       DAOTON010000275         3083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24841_length_3083_cov_3.803501, whole genome shotgun sequence.
ACCESSION   DAOTON010000275 DAOTON010000000
VERSION     DAOTON010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24841_length_3083_cov_3.803501"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="P5292_06390"
     CDS             <1..679
                     /locus_tag="P5292_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRS38791.1"
                     /translation="MRSREMHWMRIDRYYSSDADPKEGEKGDNTAMEVPSDMPRVVFQ
                     PVMCQHCNHAPCETVCPVIATSHSTEGLNQMTYNRCVGTRYCANNCPYKVRRFNWFRY
                     HDNPKFDYHMNDDLGKMVLNPDVTVRARGVMEKCSMCVQRIQAGKLEAKKAGVPVKDG
                     DINTACAQSCPTNAIVFGDYNDKNSRINKFWDPKGRSYHLLEELNVQPNVFYLTKVRN
                     SEEGKEA"
     gene            710..2095
                     /gene="nrfD"
                     /locus_tag="P5292_06395"
     CDS             710..2095
                     /gene="nrfD"
                     /locus_tag="P5292_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRS38792.1"
                     /translation="MSQANHESVVRDPLILGKKTYHQMTEDICRPIEGKANKYWWVSF
                     IIALTAMIWGLLCLSYTIGTGIGAWGANKTVGWAWDITNFVWWIGIGHAGTLISAILL
                     LFRQRWRMSINRSAEAMTIFAVICAGLFPLFHTGRPWLDYWMVPMANQFGSLWVNFNS
                     PLLWDVFAISTYFSVSLVFWYLGLLPDLATVRDRCVTPTKRFIYGLLSFGWKGTARDW
                     HRFEEVSLVLAGLSTPLVLSVHTIVSFDFATSIVPGWHTTIFPPYFVAGAIFSGFAMV
                     LTLMIIARKVLGLESYITMQHIELMNKIIILTGSIVGVAYLTEFFIAWYSGVKYEAYA
                     FQNRMFGPYWWAYWSMMTCNVISPQLFWFKKIRTSINATFILSIIVNIGMWFERFVII
                     VTSLHRDFLPSSWVMYSPTYIEVGIFVGTIGLFFTLFLIFSRVFPVIALAELKSILKK
                     TGNQFKKKSVL"
     gene            2098..2631
                     /locus_tag="P5292_06400"
     CDS             2098..2631
                     /locus_tag="P5292_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HRS38793.1"
                     /translation="MARRIYGIFDDEEILMSAIPQLKGKGVSCRDVQSPFPIHGIEKL
                     LNVPRTRISIASFMYGITGTSLALLMTWYMMIHDWPINIGGKPNFAFYMNLPAFIPVT
                     FELTVFCAAHGMAITFLLRSKLLPGVTAPIPHPRLTDDKFVLHVDVKDEGKMDEVVAN
                     LKSAGASEVLLKSLQFA"
     gene            2640..>3083
                     /locus_tag="P5292_06405"
     CDS             2640..>3083
                     /locus_tag="P5292_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRS38794.1"
                     /translation="MKKFFLYLTPALAALSLTSCVPSWDDKSKPNVEYMPDMYRSVAY
                     ETYSANPLYADSMTSRQPVSGTIARGDKVYNDYDRLPVMDNYPSTPEGYEKAGLELKN
                     PLEKSEANLAEGKRLYENYCLVCHGTSGQGDGPVPSNNGPKPPAYN"
BASE COUNT          668 a          926 c          819 g          670 t
ORIGIN      
        1 gatgcgcagc cgcgaaatgc actggatgcg tatcgaccgc tactactcaa gcgatgccga
       61 tccgaaggaa ggggagaagg gcgacaatac cgcgatggaa gtgccgagcg acatgccgcg
      121 cgtggtgttc cagccggtga tgtgtcagca ttgcaaccac gctccctgcg aaacggtttg
      181 cccggtcatc gccaccagcc acagcaccga aggtctcaac cagatgacct acaaccgatg
      241 cgtcggtacc cgctattgcg cgaacaactg tccgtacaaa gtccgtcgct tcaactggtt
      301 ccgttaccac gacaatccca agttcgacta ccacatgaac gacgacctcg gcaagatggt
      361 gttgaacccg gatgtgacgg tccgcgcgcg cggtgtcatg gaaaaatgtt ccatgtgcgt
      421 gcagcgcatc caggccggta agttggaagc caagaaagcc ggtgtaccgg tcaaggacgg
      481 cgacatcaac accgcctgcg cgcagtcgtg cccgaccaac gcgatcgtct tcggcgatta
      541 caacgacaag aacagccgta tcaataaatt ctgggatccg aaaggacgca gttatcactt
      601 gctggaagaa ctcaacgttc agccgaacgt gttctacctg accaaggtgc gcaacagcga
      661 agaaggaaaa gaagcctgat cccacaactc ataaggaatc caacacgcaa tgagccaagc
      721 gaatcatgaa tcggtcgtcc gggacccgtt gatcctcggg aagaagacgt accaccagat
      781 gacggaagat atctgccggc cgatcgaagg aaaggccaac aagtattggt gggtatcgtt
      841 cattatcgcc ctgaccgcaa tgatctgggg cctactctgc ctctcgtaca ccatcggcac
      901 cggtatcggc gcctggggcg cgaacaagac cgtcggttgg gcatgggaca tcaccaactt
      961 cgtttggtgg atcggtatcg gtcacgccgg cacgctgatc tccgccattc tgttgctgtt
     1021 ccgtcagcgc tggcggatgt cgatcaaccg ttccgcggag gccatgacga tcttcgcggt
     1081 aatctgcgcg ggtctgttcc cgctgttcca caccggccgt ccctggctcg actactggat
     1141 ggtcccgatg gccaaccagt tcggttcact ctgggtcaac ttcaactcac cgctgctctg
     1201 ggacgtattc gcgatctcga cctacttctc cgtttcgctc gtattctggt acctcggcct
     1261 gctgcccgac cttgcaacgg tacgtgaccg ctgtgtaacg ccgaccaagc gtttcatcta
     1321 cggtttgctc tcgttcggtt ggaaaggcac cgcgcgcgac tggcaccgct tcgaggaagt
     1381 ttcgctcgta ctggccggtc tttccacgcc gctcgtactt tcggtacaca ccatcgtatc
     1441 cttcgacttc gccacgtcca tcgttccggg ctggcacacc accatcttcc ctccctactt
     1501 cgtggccggt gctatcttct ccggattcgc gatggtattg acgctgatga tcatcgcccg
     1561 caaggtgctg ggactcgagt cctacatcac catgcagcac atcgagttga tgaacaagat
     1621 catcatcctg accggttcga ttgtcggggt agcctacctc accgaattct tcatcgcctg
     1681 gtactcgggt gtgaagtatg aagcctacgc gttccagaac cgtatgttcg gtccgtactg
     1741 gtgggcgtac tggagcatga tgacctgcaa cgtgatctca ccgcagctct tctggttcaa
     1801 gaagatccgc acgagcatca atgccacctt catcctgtcg atcatcgtga acatcgggat
     1861 gtggttcgag cgattcgtga tcatcgtgac ctcgctgcac cgcgacttcc tcccttcgag
     1921 ctgggtgatg tactccccga cctacatcga agtaggcatc ttcgtgggta ccatcggcct
     1981 gttcttcacc ttgttcctca tcttctcccg cgtcttcccg gtaatcgcac ttgcagaatt
     2041 gaagtcgatc ctgaagaaga ccggtaatca attcaagaaa aaatccgtcc tctgatcatg
     2101 gcaagaagaa tctacggcat cttcgacgac gaggagatcc tgatgagcgc gatcccgcag
     2161 ctcaaaggca aaggcgtatc gtgtcgcgac gtgcaaagcc cgtttccgat ccacgggatc
     2221 gagaagctgc tcaacgtccc gcgcacgcga atttccatcg catccttcat gtacggcatc
     2281 acgggtacct cattggccct gctgatgacc tggtacatga tgatccacga ctggccgatc
     2341 aacatcggcg gtaaacccaa cttcgccttt tacatgaacc ttccggcgtt cattccggtg
     2401 accttcgagc tcaccgtatt ctgcgccgct cacggtatgg ccattacgtt cctgctccgc
     2461 agcaaactgc tgccgggtgt gaccgcgccg atcccgcatc cgcgtctgac ggacgacaag
     2521 ttcgtgctgc acgtggatgt gaaggacgaa ggcaaaatgg acgaagtcgt cgccaacctg
     2581 aagagcgccg gggcttccga ggtgctcctg aaatcgcttc aattcgcctg atccgaccca
     2641 tgaagaaatt cttcctatac ctgacgccgg ccctggcagc gctttcgctg acatcctgcg
     2701 tgcccagttg ggatgacaag tccaagccga acgtggagta catgcccgac atgtaccgga
     2761 gtgtggcgta tgagacctat tcggccaacc ccttgtacgc tgacagtatg acgtcgcgtc
     2821 agccggtttc cggtacgatc gcgcgcggcg ataaggtgta caatgattac gaccgtttgc
     2881 cggtcatgga caactatcct tccactccgg aaggatacga gaaggccggt cttgagttga
     2941 agaatccgct ggagaaaagc gaggccaacc ttgctgaagg aaagcgcctg tatgagaact
     3001 actgcctcgt atgccacgga acttccggtc agggcgatgg tcctgttccg tcgaacaacg
     3061 gtccgaagcc tccggcttat aat
//
LOCUS       DAOTON010000276         3082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_24859_length_3082_cov_3.269574, whole genome shotgun sequence.
ACCESSION   DAOTON010000276 DAOTON010000000
VERSION     DAOTON010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24859_length_3082_cov_3.269574"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..299
                     /gene="secE"
                     /locus_tag="P5292_06410"
     CDS             105..299
                     /gene="secE"
                     /locus_tag="P5292_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRS38795.1"
                     /translation="MSKITGYIKDSYRELVEKVSWPTWEELQSSVIVVMVATAIIGLV
                     IFLMDLGFAGIMNLFYKITG"
     gene            314..898
                     /gene="nusG"
                     /locus_tag="P5292_06415"
     CDS             314..898
                     /gene="nusG"
                     /locus_tag="P5292_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRS38796.1"
                     /translation="MTETKQKAADNAAGKEIARKWYVVRAISGKEKKVKQYIESEVSR
                     LKLGDYVAQVLIPTEKFYQIKNGKKVSKERNILPGYVLLEAALVGEIPHIIENINGVI
                     SFLGPKGEAPVPLRQSEVNRILGKMDEMSDSEAVITVPFVVGESVKVIDGPFNSFSGI
                     IEEINEEKKKLKVMVKIFGRKTPLELNYMQVEKE"
     gene            975..1412
                     /gene="rplK"
                     /locus_tag="P5292_06420"
     CDS             975..1412
                     /gene="rplK"
                     /locus_tag="P5292_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRS38797.1"
                     /translation="MAKEIATYIKLQVKGGAANPAPPIGPALGAKGVNIMEFCKQFNA
                     RTQDQPGKVLPVIITVYTDKSFDFVIKTPPAAVQIMEAAKIKGGSKEPNRSKVGTINW
                     EQVRKIAEIKMPDLNCFTIESAMSMVAGTARSMGVTVEGDRPF"
     gene            1520..2212
                     /gene="rplA"
                     /locus_tag="P5292_06425"
     CDS             1520..2212
                     /gene="rplA"
                     /locus_tag="P5292_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007097039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRS38798.1"
                     /translation="MARLSKKRKLAMSKVDQSKSYTLAEASKLVKEVTTTKFDASVDL
                     SIRLGVDPRKANQMVRGVVTLPHGTGKTKRVLVLCTPDKEAEAKEAGADFVGLDEYIQ
                     KIEQGWTDIDVVITMPSAMGKVGKLGKVLGPRNLMPNPKTGTVTNDIGKAVKEVKAGK
                     IDFKVDKQGIIHTSVGKASFDAKKIEENANEILQTIIKLKPSAAKGTYMKSVYISSTM
                     SPSVQIDTKTLA"
     gene            2249..2773
                     /gene="rplJ"
                     /locus_tag="P5292_06430"
     CDS             2249..2773
                     /gene="rplJ"
                     /locus_tag="P5292_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRS38799.1"
                     /translation="MKREEKDVIISEITELLDKYPNVYVADTSTLTVDKTNQLRRICF
                     NKGVKMLVAKNTLIRKAMEKKNQEAYQELFAALKGTSALMFCETGNVPAKVIKEFRKA
                     ADRPALKGAWIDTAVFLGDNQLDTLAAIKSKNELIGEIIGLLQSPASNVVSALQSSGG
                     KLAGIVKTLSERAA"
     gene            2845..>3082
                     /locus_tag="P5292_06435"
     CDS             2845..>3082
                     /locus_tag="P5292_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRS38800.1"
                     /translation="MADLKAFAEQLVNLSVKEVNELAKILKDEYGIEPAAAAVAIAAP
                     GAGGGDAGGAAEKTSFDVILKSPGGAKLNVVKLVK"
BASE COUNT          852 a          826 c          815 g          589 t
ORIGIN      
        1 agagtagtgg tctccaaaac cattggctgg gagttcgagt ctctccaccc gtgcaactac
       61 atccaccacc acgaaagggt tcatcccatt atagtacctg aagcatgagt aagatcaccg
      121 gttacatcaa ggacagctac cgcgaactcg tagagaaagt cagttggcct acctgggaag
      181 aactccagag cagcgtcatc gtggtgatgg tcgcaacagc cattatcggc ctggtcatct
      241 tcctgatgga ccttggcttc gcagggatca tgaacctgtt ctataagatc accggataat
      301 tcagcaaagt gccatgaccg agaccaagca aaaagccgcg gacaacgcag ccggcaagga
      361 gatcgcccgg aagtggtacg tcgtccgtgc catcagcggc aaggagaaga aggtgaaaca
      421 gtatatcgag tcggaagtct cccgtttgaa actgggtgac tatgtcgctc aggtactgat
      481 tccgaccgag aagttttacc agatcaagaa tggtaagaag gtcagcaagg aacggaacat
      541 ccttccggga tatgtattgc tcgaagccgc actggtgggg gagatccctc acatcattga
      601 gaacatcaac ggtgtgatct ctttcctggg tcccaaaggc gaagccccgg ttccgctgcg
      661 tcagtcggaa gtgaaccgga tcctgggaaa aatggacgag atgtccgatt ccgaagcggt
      721 gatcaccgta ccgttcgtgg ttggcgagag tgtgaaagtc atcgatggtc cgttcaacag
      781 tttcagcggc atcatcgaag agatcaatga agaaaagaag aaactcaaag tgatggtcaa
      841 gatcttcgga cgtaagaccc cgcttgagtt gaattatatg caagtagaga aagagtaaac
      901 cgcaggtgag ccgataccgt tccccgcttc caggataacg gtgtcaggaa tgctcctgca
      961 caatgcatac aacgatggca aaagaaatcg caacatatat caagctccag gtgaaaggcg
     1021 gagccgctaa cccggcacct ccgatcggtc ccgcactcgg tgcgaagggc gtaaacatca
     1081 tggagttctg caagcagttc aacgcgcgca cgcaggacca gccgggtaag gtgctccccg
     1141 tgatcatcac ggtgtatacc gacaagtcgt tcgacttcgt catcaagact cctcccgcgg
     1201 ccgttcagat catggaagca gccaagatca aaggcggttc caaagaaccg aaccggtcca
     1261 aagtcggtac catcaactgg gaacaggtcc gcaagatcgc cgagatcaag atgcccgacc
     1321 tgaactgctt caccattgaa tctgccatga gcatggtggc cggcacggcc cgctccatgg
     1381 gggtcacggt agaaggggat cgcccgttct gattcctgca gacaaggagt ttacccggat
     1441 ctgatccggt caatttattc aataaccgaa ggagggtgcg atcatctcaa ccccgcttgt
     1501 acttcaaaaa cgttcattca tggctcgtct cagcaaaaag agaaaacttg ctatgtccaa
     1561 agtggaccag agcaagtcgt acaccctggc cgaagcatcc aagctggtca aggaagttac
     1621 taccaccaaa ttcgacgcat ccgtcgacct gagcatccgc ctcggggtcg atccccgcaa
     1681 agccaaccag atggtgcggg gcgtcgtgac ccttccgcat ggaaccggta agaccaagcg
     1741 cgtactcgtg ctctgcactc ccgacaagga ggcagaagcg aaagaggcgg gcgcagactt
     1801 cgtaggactg gatgaataca tccagaagat cgagcagggc tggaccgaca tcgacgtggt
     1861 catcaccatg ccgagcgcga tgggtaaagt cggtaaactc ggtaaggtgc tgggtccgcg
     1921 taacctcatg ccgaacccga agaccggtac cgttaccaac gatatcggca aggccgtgaa
     1981 agaagtaaaa gccggtaaga tcgacttcaa agtcgacaag cagggcatca tccatacctc
     2041 cgttggcaag gcttcgttcg acgctaagaa gatcgaagag aacgcaaacg agatccttca
     2101 gacgatcatc aagctgaagc cgtccgcggc caaaggcacc tacatgaagt cggtgtacat
     2161 ctccagcacg atgagtcctt ccgttcagat cgatacgaag accctcgcct gatccattca
     2221 ttaaccggtt tcaaaaacga acaatccaat gaaacgcgaa gaaaaagacg tgatcatctc
     2281 cgagatcacg gaactgctcg ataaataccc gaacgtttac gtggccgata cgtccacgct
     2341 gaccgtcgac aagaccaacc aactgcgccg tatttgtttc aacaaaggcg tcaagatgct
     2401 ggtggcgaag aataccctga tccgcaaggc catggagaaa aagaaccagg aggcctatca
     2461 ggagttgttc gctgctttaa aaggcaccag cgccctgatg ttctgtgaga cgggcaatgt
     2521 tccggcaaag gtcatcaagg aattccgtaa agcggccgac cgtcctgcac tcaaaggtgc
     2581 ctggatcgat accgccgttt tccttggcga taaccaactc gatacgctcg cagctatcaa
     2641 gtcgaagaac gaactcatcg gcgagatcat cggcctcctc cagtctccgg caagcaacgt
     2701 ggtgtccgcg ctgcaaagct ccggcggcaa actggccggt atcgtcaaga cgctctccga
     2761 gcgcgccgct taatccgaat gcttccaatc caaatatccc attacctaac taacgaaatc
     2821 taaaacaatc aaaacctaat agtcatggca gacctgaaag catttgccga acagctcgta
     2881 aacctgtcgg taaaagaagt taacgaactc gcaaagatcc tgaaggatga gtacggtatt
     2941 gaaccggccg ctgcagcagt agctattgcc gctcccggag ccggtggtgg tgatgcaggc
     3001 ggtgccgctg agaagacctc cttcgatgtg atcctgaaga gcccgggcgg cgctaagctg
     3061 aacgtggtga aactcgtaaa ag
//
LOCUS       DAOTON010000277         3061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25076_length_3061_cov_3.528942, whole genome shotgun sequence.
ACCESSION   DAOTON010000277 DAOTON010000000
VERSION     DAOTON010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25076_length_3061_cov_3.528942"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            659..1597
                     /locus_tag="P5292_06440"
     CDS             659..1597
                     /locus_tag="P5292_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRS38801.1"
                     /translation="MPDRNKKYCLLTNDVETHSIWFNTLRDETGEKVRDEGMPILLDI
                     YKKFGVKSTFYFVGDMVVKFPEVVRAILADGHEVASHGWTHEVDEAFDVLPYAKQVEH
                     LQRSRKVLEDISGQAVVSFRAPALRVNEHTPRALAEAGYRTDSSVASQRFDMFLSFGG
                     MKKLNWLKAPRLPYRTDPDNLFRKGNGPIVEVPLTALLFPFVGTTLRLFPGVTSLQQR
                     LLHAETRSNGKPMVFDVHPNEFIEEHTEHRTIARRTNNPVKYLLNDVVRGKMKVKNLG
                     RKAIPLYEQFIRFYQSRGYEFITVREYAKRQGFLND"
     gene            1609..2145
                     /locus_tag="P5292_06445"
     CDS             1609..2145
                     /locus_tag="P5292_06445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRS38802.1"
                     /translation="MPAQEVLDFYGYKGTLGKLRYAIKFVWRFFLDKLAFFCFIKSWR
                     TVIHRWRGVNIGKDVYIGHEVMFDRVFTDQIFIGDHTSVGDRTIITAHANIPSNTRLK
                     KIYPRKVMQTRIGRGVWIMPNVTIAPGITIGDEAVIATGSVVTKDVPPRCLAAGVPAK
                     VVKDFSDHEAFKSGPLPD"
     gene            2165..>3061
                     /locus_tag="P5292_06450"
     CDS             2165..>3061
                     /locus_tag="P5292_06450"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HRS38803.1"
                     /translation="MHVLIYLHHPAHFYLFKHTIRKLKADGHRLTLIATKKDVLEDLL
                     RKEGLEYRNFLPHGRKDNKWSIALGLLKQDLRLVAHCLRDRPDLMIGTSTEICHVGWL
                     LRIPNIFVNEDDAAIVPLVDKLAHPFAKHLLAPEVCVTGRPGKTITYAGNHELAYLHP
                     DLFTPDPAVKQRYIPENTPYFLLRFAKLGAHHDTNAKGISDAIARKLIELLEPHGRVY
                     ITSERALAPEFEPYRLNIHPIDIHHIMAFSSLFIGDSQTMSAESGVLGVPFIRFNAFA
                     GKIGYLVELEEKYRLGVSIHPDN"
BASE COUNT          709 a          875 c          806 g          671 t
ORIGIN      
        1 gctgtaccgt ttcttccggg aagcaccctg ctttctgggt tgttgccagg caagcgcggc
       61 attaccgtta ccgctgaaca cattccagcg aaggtcctgc agcaggttcc ggttattggt
      121 ggtccttccg ttcttcttca tagcgcagcc ctacaagggg gaatagcaaa ttaccccgtg
      181 gtaaagcgga cgcaaacgaa actcagccga atcattacag gattaaggca gtcttcggta
      241 ttccggaatg aaccccattc atttccgtcg aagatccttg gtgagccggg atggatagaa
      301 acggttcgca gccgctatcc cagtcctggt gtgtgtttca cgtaagggcc ttgatgcttc
      361 ccctggtact cgattgttgc tgtttgcagt ttcgaatata gtgcaaggat tcagttggag
      421 caacctttct tgtagaaaat ccgcttccag cgcggtcttt gggctaaatc acgcgatttt
      481 cttacggttt gacgattttc ggggctgtaa ttttctacct gaaaaaaagc cgaaattgcg
      541 cgaaaagccc gcggcgggag caacctactc cgtcaggaat tagtataata tcagactggg
      601 aaagggcttt tttccctgga gaccgcgtgt tcgcaaagcc cgctacccgc tcacccgaat
      661 gcctgataga aacaagaaat attgtctgct gaccaacgat gtggaaacac attcgatctg
      721 gttcaatacg ctgcgcgacg aaaccggcga gaaggtacgc gacgagggca tgccgatcct
      781 gctggacatt tataaaaagt tcggcgtgaa gagtacgttt tactttgtgg gcgacatggt
      841 ggttaagttt ccggaagtcg tgcgtgccat cctggccgac ggtcacgaag tggcctcgca
      901 tggctggacc cacgaagtgg atgaagcatt cgacgtgctg ccgtatgcga aacaagtcga
      961 gcacctgcag cgttcgcgca aggtcctgga agacatttcc ggtcaggcgg tcgtttcgtt
     1021 tcgtgcacca gcgctgcgtg tcaacgaaca tacgccccgc gcattggcgg aagcgggtta
     1081 ccgcaccgat agttccgtgg cttcccaacg cttcgacatg tttctgtcgt tcggcggcat
     1141 gaagaaactg aactggctca aggctccgcg cctaccctac cgcaccgatc ccgacaatct
     1201 ctttcgaaaa ggtaacggac cgatcgtaga ggtaccgttg accgcactgt tgttcccatt
     1261 cgtagggaca acgctacgcc tctttccggg tgtcaccagt ctgcaacaac ggctgttgca
     1321 tgccgagacc cgttccaacg gaaaaccgat ggtattcgac gtgcatccga acgaattcat
     1381 cgaagaacat accgaacacc gcaccatcgc acgacgaacc aacaacccgg tcaaatacct
     1441 cctgaacgat gtggtccgtg ggaaaatgaa agtgaaaaac ctgggacgca aagcgatccc
     1501 gctgtacgaa caattcatcc gcttctatca gtctcgcggg tatgaattca tcaccgtacg
     1561 cgagtacgca aagcgacaag ggtttctgaa cgactaatca cacgttgtat gccggcacaa
     1621 gaagtactgg atttttacgg atacaaaggc acgctgggca agttgcgcta cgcgatcaaa
     1681 ttcgtctggc ggtttttcct tgacaagctc gcgttcttct gtttcatcaa aagctggcgt
     1741 acggtcatcc accggtggcg cggcgtgaac atcggcaagg acgtgtacat cggtcatgaa
     1801 gtgatgttcg accgcgtctt caccgatcag atcttcatcg gcgatcacac ctccgtaggc
     1861 gaccggacca tcatcaccgc gcacgcgaac attccgtcga atacacgatt gaaaaaaata
     1921 tatccgcgca aagtcatgca aacccgcatc gggcgcggcg tctggatcat gccgaacgtc
     1981 accatcgctc cgggcatcac catcggcgac gaagcggtga ttgcgaccgg ctcggtggta
     2041 acgaaggatg tgccaccccg ttgtcttgcg gccggggttc ctgccaaagt ggtgaaggac
     2101 ttttccgatc acgaagcgtt caagagcggt ccactgcccg actgaccgca attactgcca
     2161 aggcatgcac gtactgatct atctgcacca tccggcgcat ttctatctct tcaagcacac
     2221 gatccgcaag ctgaaggccg acggtcatcg tctcacactg atcgcgacga agaaggacgt
     2281 actcgaagac ctgttgcgca aggagggact cgaataccgg aactttcttc cgcacggacg
     2341 taaggacaac aagtggtcga tcgcgctggg attgctcaag caggatctcc gcctggttgc
     2401 ccattgcctc cgcgaccgtc cggacctgat gatcgggacc tcgacggaga tttgtcatgt
     2461 cggttggttg ttgcgcattc cgaacatctt cgtgaacgaa gacgatgccg cgatcgtgcc
     2521 gctggtcgac aagctggctc atccgtttgc caaacacctg ttagcgccgg aagtctgcgt
     2581 aaccggacgg cccggcaaga ccatcaccta tgccggcaac cacgaactgg cctacctcca
     2641 ccccgatctg ttcacgcccg atcccgccgt caagcagcgt tacataccgg aaaacacgcc
     2701 ctactttctc ctgcgcttcg ccaagctggg cgcgcaccac gacaccaacg ccaaagggat
     2761 cagcgacgcg atcgcgcgta aactcattga attgctggag ccgcacggaa gagtgtatat
     2821 cacttcggaa cgcgcgctgg ctcccgagtt cgagccctac cgcttgaaca tccatccgat
     2881 cgacattcac cacatcatgg ccttttcatc gctgttcatc ggtgacagcc agaccatgtc
     2941 ggcagaatcc ggggtgctgg gcgtaccgtt cattcgcttc aatgccttcg ccgggaagat
     3001 cggttacctg gtcgagctgg aagaaaagta tcgcctgggt gtcagcattc accccgataa
     3061 c
//
LOCUS       DAOTON010000278         3053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25175_length_3053_cov_2.685123, whole genome shotgun sequence.
ACCESSION   DAOTON010000278 DAOTON010000000
VERSION     DAOTON010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25175_length_3053_cov_2.685123"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="P5292_06455"
     CDS             complement(<1..1186)
                     /locus_tag="P5292_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38804.1"
                     /translation="MHRPSYFHRVSGTSFSPQQPFLLRTFSIALLAAAGLAGVFAGPL
                     LLSWIEIRLHAALDTSFWSEGFIASGWWLLTFAASLLLVRSSVHSPIYRWVNPLLLLL
                     WFGYFLIIQFRHPILLPFFDDYTGFLDDYSLILSGNYPLTRIEWLWQTYWECRVPIPR
                     LLFILLSACFGNQVFLAAKLLTTLLLALVGYLLLLNTEARKKPVTSLLILGGLLHFGF
                     FFSSLSGLSGTCYYGSLLGSLWALHLQHRNRSIGLVLLAATLGALCFASGFVVFPVLL
                     YRAWKAGAKRSIGWIAAWTALFCALYFYGYHPQRLNALDGFFPIAFLAFIPIFLGNSL
                     QFLYHWSLPLLGGSVLTWIGVQHLRVKNAGYYADVLLVLIGIAAMTAFFRHPAGVEWA
                     LNI"
     gene            complement(1214..2890)
                     /gene="pruA"
                     /locus_tag="P5292_06460"
     CDS             complement(1214..2890)
                     /gene="pruA"
                     /locus_tag="P5292_06460"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HRS38805.1"
                     /translation="MSNAISLVPVAVNEPVLNYAPGSPERESLKAAIRKMRSEVRDIP
                     MYINGKEVRTGKTSAVRPPHDHQHVVGNFHIGGKEHVEQAIEAALAARQAWSDLPWEQ
                     RAAIFLKAADLLAGPYRAIINAATMIGQSKNVYQAEIDAACEMIDFLRFNVQYMRQIY
                     TEQPISSKGIWNRLEHRGLEGFVFALTPFNFTAISGNLPTSPAMMGNTVVWKPANTQV
                     YAASVLMQVFMEAGLPPGVINLVYVSGPVAGDVIFQHPDFAGIHFTGSTGVFQDIWKT
                     IGTNIHRYKAYPRIVGETGGKDFIMAHPSADVPALVAAITRGAFEYQGQKCSAASRVY
                     IPKSLWPEVKKGLVNDINSFKIGPTEDFSNFVNAVIDEKSFDKLAKYIDDAKKSPDVE
                     VVVGGTYDKSKGWYIHPTVLLAKDPKYVTMCEELFGPVLTVYVYEDAQYEQTLSLVDQ
                     TGTYALTGAIFSQDRYAIDLAMRKLTHAAGNFYINDKCTGAVVGQQPFGGGRASGTND
                     KAGSILNLLRWVSPRAIKETFVPPTDYRYPFLQEENANGSPVRQQAEAAK"
BASE COUNT          673 a          801 c          998 g          581 t
ORIGIN      
        1 ggatgttcag ggcccattcg actccggcgg ggtgacggaa gaaagccgtc atggccgcga
       61 tgccgatgag caccagcaag acatcggcat agtatccggc atttttaacg cgcaggtgct
      121 gaacgccgat ccaggtcagg accgatccgc ccagcagcgg cagtgaccag tggtagagga
      181 actgcaggct gttgcccagg aagatcggga tgaacgccag gaaggcgatc gggaagaagc
      241 cgtcgagggc gttcagccgc tgcgggtggt atccgtagaa gtacagagcg cagaacagcg
      301 cagtccaggc ggcgatccag ccaatcgacc gtttagcgcc tgccttccag gcccgataca
      361 acagcaccgg aaaaacgacg aagcccgagg caaagcaaag cgctccaagt gtagcagcca
      421 gtaacaccag accgatcgat cggttgcggt gttggaggtg gagtgcccag agcgaaccca
      481 gcagactgcc gtagtagcag gttccggaaa gtccgctcag cgaagagaag aagaaaccga
      541 agtggagcaa tccaccgagg atcagcaggc ttgtcaccgg ttttttacgg gcttcggtat
      601 tgagcaggag caggtagccg acgagtgcca gcagcagggt tgtcagcagt ttggcggcta
      661 agaagacctg gttgccgaaa caggccgaca gcaggatgaa cagcaggcgc gggattggca
      721 cgcggcattc ccagtacgtc tgccacaacc attcgatccg ggtaagcgga tagttaccgg
      781 acaggatcaa cgagtaatcg tcgaggaagc cggtgtagtc gtcgaagaag ggcaggagga
      841 tcggatggcg gaactggatg atcaggaagt atccgaacca cagcagcaac aagagcgggt
      901 taacccatcg gtagatgggg ctgtgtaccg aggaacggac cagcaaaagt gaagcagcga
      961 acgtcagcaa ccaccagccg gaagcgatga agccttccga ccagaaggaa gtgtcgagcg
     1021 cggcgtgcag ccggatctcg atccagctca ggagtagcgg accggcgaat acgcctgcca
     1081 gtcccgcagc ggcaagcaac gcgatgctga aggttcgcaa caggaaaggc tgttgaggag
     1141 aaaaggacgt gccgctcacc cggtgaaaat aagaaggccg gtgcattcag caccggcctt
     1201 tgtaaaggtt tgatcacttc gcggcttccg cctgttggcg gaccggactg ccgttggcgt
     1261 tttcttcctg gaggaagggg tagcggtaat ccgtcggggg aacgaaggtt tccttgatgg
     1321 cgcggggcga aacccagcgc agcaggttga ggatggaacc cgccttgtcg ttcgtacccg
     1381 aagcgcggcc cccgccgaaa ggctgctggc cgaccacggc gccggtacat ttgtcgttga
     1441 tgtagaagtt accggccgcg tgcgtcagtt tacgcatggc caggtcgatg gcatagcgat
     1501 cctgcgagaa gatagcgccg gtgagggcat aggtgccggt ctggtcgaca agcgaaagtg
     1561 tctgctcgta ctgcgcgtct tcgtacacat agaccgtgag gaccggacca aacagctctt
     1621 cgcacatggt cacatacttc gggtctttcg cgagcagcac ggtcgggtgg atgtaccagc
     1681 ccttgctctt gtcgtaggtg ccgccgacca ccacttcaac gtccgggctc tttttggcat
     1741 cgtcgatgta cttcgcgagt ttatcgaacg acttctcgtc gatgaccgcg ttgacgaaat
     1801 tcgaaaaatc ttcggtcggt ccgatcttga aggaattgat atcgttgacc agccccttct
     1861 tcacctcggg ccagaggctc ttcgggatat agacacggga agcggcggaa catttttgtc
     1921 cctggtattc gaacgcgccg cgggtgatgg cggctaccag cgcgggaaca tcggccgagg
     1981 ggtgggccat gatgaaatcc ttgccaccgg tctcgcctac gatgcgcgga taagctttgt
     2041 aacgatgaat gttcgtgccg atcgttttcc agatatcctg gaagacaccg gtagagccgg
     2101 tgaagtggat gccggcgaaa tcgggatgct ggaagatcac atcaccggca acggggccgg
     2161 agacatacac caggttgatg acgcccggag gaagtccggc ttccatgaac acctgcatga
     2221 ggaccgaagc ggcatatacc tgcgtgttgg ccggtttcca cacgaccgtg ttgcccatca
     2281 tggccggcga ggtcggcagg ttgccgctga tggcggtgaa gttgaagggc gtgagcgcga
     2341 agacgaagcc ttcgaggccg cggtgttcga ggcggttcca gatacctttg gaagagatcg
     2401 gctgttcggt gtagatctgg cgcatgtact gcacgttgaa gcgcaggaag tcgatcatct
     2461 cacacgcggc gtcgatctcg gcctggtaca cgttcttgct ctgaccgatc atggtcgcgg
     2521 cgttgatgat cgcgcggtag gggccggcga ggaggtcggc tgctttcaga aagatcgcgg
     2581 cgcgttgttc ccagggaagg tcggaccagg cctgacgggc ggcgagggct gcctcgatcg
     2641 cctgttcgac gtgctcttta ccaccgatat gaaaattccc gacgacatgc tggtgatcgt
     2701 gcggcggacg aacggcggaa gtcttgccgg tgcgcacttc cttgccgttg atgtacatcg
     2761 gaatgtcgcg cacctcgctc cgcatcttgc ggatggcggc tttgagggat tcgcgttcgg
     2821 ggcttccggg agcgtagttg aggacaggct cgttgacggc taccggtaca agggaaatgg
     2881 cgttcgacat ggggtctgaa tttcggtgca aaggtacgga attcgacccg ttaaggatat
     2941 gtccgacgtc agcttcgagc gttcactaat ttcgtttctg tagcagattc gcccatttcc
     3001 gacctagttc gagcgcagga cgttctatga atcggtgcag cagccaggcg aaa
//
LOCUS       DAOTON010000279         3048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25231_length_3048_cov_3.345139, whole genome shotgun sequence.
ACCESSION   DAOTON010000279 DAOTON010000000
VERSION     DAOTON010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25231_length_3048_cov_3.345139"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..982)
                     /locus_tag="P5292_06465"
     CDS             complement(125..982)
                     /locus_tag="P5292_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38806.1"
                     /translation="MALTRNGSHQLSGLSRFHGAVGAYGLRSALDNNGTRKNFDSGAH
                     TVSGVTNKAAIPDGFRHPMSWKMGTKAGSLSSHVEADLTVTGAADGLRGLPGEASASF
                     SITTNTPSGELIVSGTGTAALLLDTNTPLLTASLNGAGSASFTVETNIPLLGAEASLV
                     GTTTMVISFADADILPLDDSPVLRDATASFSFAGTLQSYAVGYMEGTTEEAGLTPTGI
                     ANTVWAKVIEAGYSAEQILRLLAAYAAGSATGLEGANPQFTGLDGVTLRIDGAYSAGT
                     RTIDALDGD"
     gene            complement(984..1901)
                     /locus_tag="P5292_06470"
     CDS             complement(984..1901)
                     /locus_tag="P5292_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38807.1"
                     /translation="MGFQSSDDLINQITVNGKYGRIDYNKTTAVAGVAGTWTDLGTAT
                     GSIPANTYAGTSLTWVDTDDTWAEGAIYHGGDVSTATKHFLNASASVFAAAGAPWILM
                     CVDQVGYVPITGADVTGTSERTVSMTAIGSSAAKRDRHANGVGLRANFSAEVAPSTGG
                     PNLTSFKYTDQAGNTAQNMTTAVGFAAAAGAVTGAIPHSGNAATRYAPFLPLLPGDTG
                     IKDIENFTLSGGTAYTGSGQLVLHLVKPLWQIPIPASGILTERDFVNQLPSLPQIKDG
                     ACLRFLLFQTGATTNTSPLIVAADYAYGG"
     gene            complement(1906..2775)
                     /locus_tag="P5292_06475"
     CDS             complement(1906..2775)
                     /locus_tag="P5292_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38808.1"
                     /translation="MFKNFRTLGQSFDEGRSWISTFRKVPAATATITGQWYDYSYASG
                     NPIPNYYAASPLVSAVLESDKGIIVPRMDSGQKQYLHRLSVMSSGATASTQPIYMLDY
                     LLYYPFVDMDAAGEEQLMTASTPLPRYASGVGVQMMVIAQSPTVGGGRFTITYIGSDD
                     AQYTTTSMFCGAAQPSGAVVNAVLGTGGLTPFVPLNAGVKGVKSVVSCNFSVANGGLC
                     AIVLVMPLETTIALESTSVAGLGAAVEKEALRLRGGIVEIMDGAFLGLLGQGVAGSLA
                     SSPLVGTIETVWS"
     gene            complement(2775..>3048)
                     /locus_tag="P5292_06480"
     CDS             complement(2775..>3048)
                     /locus_tag="P5292_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38809.1"
                     /translation="SNEATYTGYARVALTKSSAWTGTSSPFTNTSLIQFGACSGGSNT
                     ITHFAVVDTASGAVSMMISGALSSSLAVSSGIQPQFAGGALSISAD"
BASE COUNT          623 a          899 c          921 g          605 t
ORIGIN      
        1 taggcgctgc ctgctgcaaa caggttgagt gaagcggtgc cattgatgag accgggatca
       61 atcggcccta tcagccgacc atcccattta ccctgccatt ggcctaccca attccctatc
      121 atggctagtc accgtccagg gcgtcaatag tccgcgttcc ggcggaatag gctccgtcaa
      181 tccgcaacgt gaccccgtca agcccggtaa attgcgggtt tgcaccttcg aggccggttg
      241 ccgatcctgc tgcatacgct gcaagcaggc gaaggatttg ttccgccgag tacccggctt
      301 cgataacttt ggcccatacc gtgttggcga tgcccgttgg cgtcaggccg gcttcttccg
      361 tcgtgccttc catataccca acggcgtaag actggagtgt tcccgcgaaa gaaaacgaag
      421 cggttgcatc ccgaagcaca ggactgtcat ccaacggaag aatgtcggca tcggcgaagg
      481 aaataaccat cgtcgttgtg ccgaccagac tggcttctgc accaagcaga gggatgttcg
      541 tttcaaccgt gaagctggcc gagccggccc cattgagcga tgcggtaagc agcggggtgt
      601 tcgtgtccag cagcagcgca gccgtgccgg ttccggagac gatcagttct ccgctcgggg
      661 tattggtggt gatactgaac gaggctgatg cctcacccgg aaggccacga agcccgtccg
      721 ccgcgcccgt aactgtaagg tcggcttcaa catgggaaga aagggaacct gccttggtgc
      781 ccattttcca cgacatcggg tgacggaatc cgtcagggat cgcggccttg ttggtcacgc
      841 cggataccgt gtgcgccccg gagtcgaagt tcttgcgcgt gccgttgttg tccaacgcag
      901 accgtaaccc atacgcaccg accgccccgt ggaagcggga caagccggag agttggtgcg
      961 agccgttgcg cgtcagggcc atgttacccg ccgtatgcgt agtcggcagc gacgatcagc
     1021 ggcgaagtgt tcgtggtcgc gccggtctgg aacagcagga agcgcaagca cgcgccgtcc
     1081 ttgatctgcg gcaggctcgg caactggttc acgaagtcac gctctgtcag gatgccggaa
     1141 gccgggatcg ggatttgcca tagcggcttg accagatgca ggacaagctg ccctgaaccc
     1201 gtgtaggccg taccgccgga aagcgtaaag ttctcaatgt ccttaatgcc cgtgtctcca
     1261 ggaagaagcg gcaagaaagg tgcgtagcgt gttgctgcgt tgcctgagtg tgggatcgca
     1321 cccgtaaccg caccagcagc ggcagcgaaa ccaaccgccg tggtcatgtt ctgcgcagtg
     1381 ttccccgcct ggtcggtgta tttgaacgag gtcaggttag gaccgccagt agatggcgcg
     1441 acctctgccg agaagttcgc tcgcaacccg acgccgtttg cgtggcggtc gcgcttggcg
     1501 gcggaacttc cgatagccgt catggagacg gtacgttcag atgtacccgt aacgtccgca
     1561 ccagtgatcg ggacgtaccc gacctgatcg acgcacatga gaatccacgg agcacctgct
     1621 gctgcgaaca cggaggcgga agcattcagg aagtgcttgg ttgcggtcga cacgtcgccg
     1681 ccgtggtaaa tcgcgccttc cgcccacgta tcgtcggtat cgacccacgt cagcgaagtg
     1741 ccggcgtagg tatttgcggg aatggaaccc gttgccgttc cgaggtccgt ccaggttccg
     1801 gccacgcccg ctacggcagt tgtcttgttg tagtcgatgc ggccatattt gccgttgacg
     1861 gtgatctggt tgatgagatc atcactgctt tggaatccca ttgtcttaac tccagactgt
     1921 ttcgatagtg ccgacaagcg gcgaagatgc gagtgaccct gcgacgcctt ggcccaatag
     1981 cccaaggaat gccccgtcca tgatttccac gatgccgcca cgaaggcgca acgcttcttt
     2041 ctcgactgcc gcaccaaggc ccgccacact ggttgattcg agggcgattg tggtttccag
     2101 tggcatgacg aggacgatgg cgcacaagcc accgttcgcc acggagaagt tgcaggagac
     2161 gaccgacttg acgcctttga cgccggcatt cagcggcacg aacggagtca gcccgcctgt
     2221 accaagcacc gcattgacta cggcccctga cggctgtgcg gccccgcaga acatgctcgt
     2281 cgtggtgtat tgcgcgtcat cgcttccgat gtaagtaatc gtaaatctcc cgccgccaac
     2341 ggtaggggac tgcgcgatta ccatcatctg cacaccgacc ccgcttgcgt atctcggcag
     2401 gggagtagat gcggtcatca actgttcttc cccagccgcg tccatatcaa cgaacgggta
     2461 gtaaagcagg tagtcgagca tataaatcgg ctgcgtactg gccgtcgctc cgctactcat
     2521 cacagacagg cgatgcaggt attgcttctg cccggagtcc atgcgcggca caatgatgcc
     2581 cttgtccgat tcaagcacag ccgagaccag aggactcgct gcgtagtaat tcgggatcgg
     2641 gtttcctgat gcgtaagaat agtcgtacca ctggccggtg atggtcgccg tggcagcagg
     2701 aaccttgcgg aacgtgctga tccacgaacg cccttcgtca aacgactggc cgagggtacg
     2761 aaaattcttg aacatcagtc ggcagagatc gacagtgcgc cgccggcaaa ctgcggttga
     2821 atgccactag acactgcaag cgacgaggac agcgcgccgg aaatcatcat gctcaccgca
     2881 ccggaggctg tgtcgacaac ggcaaagtgc gtaatcgtat tggacccccc ggagcacgca
     2941 ccgaactgga tcaggcttgt gttggtgaac ggcgaggatg ttccggtcca ggcgctcgat
     3001 ttggtcagcg caacacgcgc atagcccgtg taagttgcct cgttggag
//
LOCUS       DAOTON010000280         3034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25348_length_3034_cov_3.185297, whole genome shotgun sequence.
ACCESSION   DAOTON010000280 DAOTON010000000
VERSION     DAOTON010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25348_length_3034_cov_3.185297"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1837)
                     /locus_tag="P5292_06485"
     CDS             complement(<1..1837)
                     /locus_tag="P5292_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HRS38810.1"
                     /translation="MMQSQQPIPELQQEQEEQGIDLRYFFFNYVLRYWYLYIISLALA
                     LIVAYYYTWYVTPIYRASATVMVKQPKSSTDAADILKQLDDFTTDRNIQNEIEVLRSR
                     GLIMKTVKDLNFDISYYLKGFVKTSERYNDAPFRFRPDTLNYFAYSTPVHIRILNDAK
                     FEFGYFDPREEKEITGTYRFGEKITNRIGVFSLEKMDFFNNTAFNNPKFEKRNYILRF
                     NNPERLVDQYQSALEVAIPNKQSSILRLSLENAVPDKAVDFLGKLIEVWLQSNIEDKN
                     ELARNSLNFIDEQLAIITRDLNEVEGEYEKFRSEKGITDVQTEAQSYLESAKYYDSRI
                     SEIDLKLSFLNYLENYVRQEKDLKGLSPASIGIDDALLLKLITQLNDLENQRSLLSTS
                     ATSDNPRMIELNLAIQNTKTDLLENIKSIRAGLKASQSEATTQLSRVEGKIRLLPGSQ
                     RVIVNIERQRDVKAALYTYLMQKRAETAIILASTISDNKLIDRPSAGLTPVRPVKMQI
                     YAIAILLGLLLPVAFTYLRDALNDKIQDRVSLERSTSIPMLGIVGLSDITTEMIVREK
                     PKGYIAEAFRSIRTNLQYFNPDRKKCFTVLVTSSIPAEGKSFCALN"
     gene            complement(2110..>3034)
                     /locus_tag="P5292_06490"
     CDS             complement(2110..>3034)
                     /locus_tag="P5292_06490"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38811.1"
                     /translation="ATVSTTSGFTSGSVCVIAVGVCGSPVSNSPQRCKSVVSGKLNTP
                     GNITGDPRTGVCGQTYTYSIPALASATSYTWTLPAGATGSSSTNSITVTFSGSFTSGQ
                     ICVYGNNACGAGFQRCVTVTGNPANPTGLTTTSNTPCIGTDILVSWNAAPGATSYDVL
                     VPVGSTVLTGTPTTNTFAVINVGSTGGVVGIRARSSCGNSGTATLAIAPVACRASGSL
                     ANDAVATRLELFPNPAKDRAQIRFEAPAHENVTLEAYDAAGRRVKGFSFTAEQGLNLR
                     ELDLAGMTPGMYFFVINGNELGMTRFTLIVE"
BASE COUNT          687 a          781 c          827 g          739 t
ORIGIN      
        1 ggttgagtgc gcaaaagctc ttcccttccg ccggaatgga agaggtcacg aggaccgtaa
       61 aacacttctt tcgatcggga ttgaaatact gaaggtttgt ccgaatggaa cgaaaggctt
      121 ccgcgatgta tcccttgggc ttctcacgaa cgatcatttc cgtggtgatg tcgctcaatc
      181 cgactatccc caacatcggg atggatgtgg agcgctccag cgacacacgg tcctggatct
      241 tgtcgttgag cgcatcgcgg aggtaggtaa aggctaccgg cagtaacaag cccaggagaa
      301 tagcgatcgc gtagatctgc attttgaccg gacgcacggg agtaagtcct gccgacggac
      361 gatcgatcag tttattatcc gaaatggtag atgccaggat gatggccgtt tccgcacgtt
      421 tctgcatgag atacgtgtac aaggccgctt tgacatcgcg ctgacgctcg atgtttacga
      481 ttacgcgctg cgagccggga agcaaccgga tcttcccttc caccctgctc aactgggtcg
      541 tcgcctcgga ttgcgaagct ttcaggcccg cccggatgct tttgatattc tcgagcaggt
      601 ccgtcttggt attctggatt gccaggttca actcgatcat ccgggggttg tcggaagtcg
      661 cgctggtgct gagcaggcta cgctggtttt ccaggtcgtt gagctgcgtg atcagcttga
      721 gcagcaaggc atcatcgatt ccgatacttg caggagaaag tcccttgagg tccttttcct
      781 gacggacgta attctcgaga taattcagaa agctcagttt gaggtctatt tctgaaattc
      841 gcgagtcgta atattttgcg ctctccaggt acgactgggc ttcggtctgt acgtcggtga
      901 tacccttttc cgagcggaat ttctcgtact ctccttccac ttcattcagg tcgcgtgtga
      961 tgatcgcgag ttgttcatcg ataaagttca gtgaattgcg cgcgagctcg ttcttatcct
     1021 caatattcga ttgcaaccaa acctcgatca gcttgccgag aaaatctacg gccttatccg
     1081 gtacggcatt ctccagcgac aagcgcagga tcgatgattg tttattggga atggcgactt
     1141 ccaacgcgga ttgatactga tcgaccaggc gttcgggatt attgaaacgc aagatgtaat
     1201 tgcgcttctc gaacttggga ttgttgaacg ccgtgttgtt gaagaagtcc atcttctcca
     1261 gcgagaatac tccgatccga ttggtgatct tctctccgaa gcgatacgtc ccggttatct
     1321 ccttctcctc ccggggatcg aaatatccga attcgaactt ggcatcattg aggatgcgga
     1381 tgtgtaccgg tgtcgaatag gcgaaataat tcaacgtatc cggcctgaac cggaaggggg
     1441 cgtcattgta tcgttcagag gtcttgacga atcccttcag gtaatagctg atatcgaaat
     1501 tgagatcctt cacggtcttc atgatcagac cgcgggaacg caacacttcg atctcattct
     1561 ggatgttacg atcggtcgta aaatcgtcaa gctgcttcag gatgtccgcc gcatcggttg
     1621 aactcttcgg ctgcttgacc atgaccgttg cggaagcacg atagatcggc gtcacgtacc
     1681 aggtataata gtacgctacg atcagggcca gggcgaggct gatgatatac aagtaccaat
     1741 accgcaggac ataattgaaa aagaagtaac gaaggtcaat gccctgttct tcctgttcct
     1801 gttgcagttc cggtatgggc tgctgcgatt gcatcattga aggatattcg gactgcttcc
     1861 aaacgtggag attccccgcg tttgcaagcc gacctgttaa ctaatcaatc gtccaatact
     1921 caggaaacat caattgcttc cgaatgccga cattttatcc cgattgttta ctgaaaaagg
     1981 ccggaattgt ttcatctgct ttgcttaccg cgcctgcagg acaagaaaaa aaacatcgaa
     2041 aacgtcccaa aaagcgagac gcagccccat ctttcgacaa ggctgcgtct ctggttcgca
     2101 gttttccaat tactccacta tcaaggtgaa tcgggtcatt cccagttcat ttccattgat
     2161 cacgaagaag tacatacccg gggtcatgcc tgccagatcc aattcccgaa ggttaagacc
     2221 ttgttcggca gtgaaactga atcccttcac acgtcgtccg gccgcatcgt aggcttcgag
     2281 cgtcacgttt tcgtgtgcag gtgcttcgaa tcggatctgt gcacggtcct tcgcaggatt
     2341 cgggaacagc tcgaggcgcg tagctacggc atcattcgcc aggcttccgg atgcacggca
     2401 agcgacagga gcgattgcca gggtggcggt tccggagtta ccgcagctgc tgcgagcacg
     2461 gataccgaca acacctccgg tagaaccgac gttgatgacg gcgaaggtat tcgtggtcgg
     2521 ggttccggtc aggacagtcg aaccgaccgg taccagtaca tcatacgagg tagctccggg
     2581 agcggcattc cagctcacca gaatatcggt gccgatgcac ggggtattcg atgtggtggt
     2641 caatccggtc gggttggccg ggttgcccgt gacggtcacg cagcgctgga atcctgcacc
     2701 gcaggcgttg tttccataga cacagatctg tccggaagta aagcttccgc tgaaggtcac
     2761 cgtgatggaa ttcgtgctgg atgagccggt cgcacctgca ggcaaggtcc aggtgtagct
     2821 ggtagcgctt gcaagcgccg ggatcgaata cgtgtaggtc tggccgcaga caccggtacg
     2881 cggatcaccc gtgatgttac ccggagtgtt cagcttgccg gaaacgaccg acttgcaacg
     2941 ctgaggcgag ttggaaacag ggcttccgca aacaccgact gcaatcacac ataccgaacc
     3001 ggaggtgaat ccgctagtcg tcgataccgt ggcc
//
LOCUS       DAOTON010000281         3033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25355_length_3033_cov_4.267293, whole genome shotgun sequence.
ACCESSION   DAOTON010000281 DAOTON010000000
VERSION     DAOTON010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25355_length_3033_cov_4.267293"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1458)
                     /locus_tag="P5292_06495"
     CDS             complement(55..1458)
                     /locus_tag="P5292_06495"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HRS38812.1"
                     /translation="MFKRALLLLLLPLLSLAQLPKDGPVKYYYPNGNVSSEGTLRNGK
                     PDGYWKSYSESGRIKSEGNRVDFKLDSLWRFYNDSGIVTAEYNYKEGLKNGLQRTYWD
                     NGKLQSEYTDSMSVKNGLLKSYNEDGKIIKVVPYTSGVENGLAREYRADGTLITVTYY
                     RNGYFQKEERINRVDKNGMKQGLYKDFYDTDQLLREGTYKDDKKDGFFKEYSLDGRVV
                     RKEEYRMGELVVKEEEEKDKFEVKRGYYETGAVKIVGTYKKGVPEGVFRKYDEQGNID
                     SAKVYSGGRLLRQGKMDNQGREQGEWKEFYESGKLRAIGNYVDGKREGPWKFSFENDT
                     LEQTGTFVKGKPNGPWKWYYPNGQLRREETYANGKENGYMTEYSDSGTVIAEGNYVNG
                     LQDGDWKYRNGEYLAEGKFTEGKEDGRWKQTYPNGKLAFEGEYLDGQETGVHKYYWPN
                     GKEREERTYRLGLLDGV"
     gene            complement(1569..2582)
                     /gene="nadA"
                     /locus_tag="P5292_06500"
     CDS             complement(1569..2582)
                     /gene="nadA"
                     /locus_tag="P5292_06500"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRS38813.1"
                     /translation="MTDTLLTAEEIRSVDRKGWLDRKVDPTLDLFAEIARLKKEKNAV
                     LLAHYYQDADIQDVADYIGDSLGLAQQAEKTTASMILFAGVHFMAETAKILNPDKKGV
                     LPDLRAGCSLADSCPPDAFAKFKAEHPGHVVISYINCTAEIKAMSDIICTSSNAIAIV
                     NSVPKDTPIIFAPDKNLGRYIMEKTGRPMVLWNGSCMVHEIFSLEKIVRLKAAHPNAK
                     FIAHPECEEAVLKIADYIGSTTGLLKYTVNSPDQEFIVATETGILHQMMKASPKKTFI
                     PAPPNNACACNDCPHMKLNTLEKVYLCLKYEVPELTMDEDLRKKALLPIQRMLEISRQ
                     QGL"
BASE COUNT          545 a          808 c          859 g          821 t
ORIGIN      
        1 gccgttgcgg aaggtcatcg ttacgatcgg tgcgccgcct tcgtcgaaaa ctttttagac
       61 gccatcgagt aatcccaggc gataggtgcg ctcttcgcgc tctttgccgt tgggccaata
      121 gtacttgtgt acgcccgttt cctgcccgtc gaggtattcg ccttcaaacg cgagcttgcc
      181 gttgggatag gtctgcttcc agcgaccgtc ttcttttcct tcggtgaact ttccttcggc
      241 caggtattcg ccattgcggt atttccagtc gccatcctgc aggccgttca cgtagttgcc
      301 ttcggcgatc acggtgccgc tgtcggaata ctcggtcatg tacccgttct ccttgccgtt
      361 ggcataggtc tcctcccggc gtaattgtcc gttcgggtag taccacttcc agggaccgtt
      421 cggctttcct ttcacaaagg tgccggtctg ctcgagcgta tcgttctcga agctgaactt
      481 ccacggacct tcccgcttgc cgtcgacgta gttgccgatc gcgcgcagct ttccgctctc
      541 gtaaaactct ttccactcgc cctgctcgcg accctggttg tccatcttgc cctgtcgtaa
      601 caagcgtccg ccggaataga ctttcgctga gtcgatgttg ccctgctcgt cgtacttgcg
      661 aaagacgcct tcgggtacac ctttcttgta ggtgccgacg atcttgaccg ccccggtctc
      721 gtaatagccg cgcttgactt cgaacttgtc cttctcttct tcttccttca ccaccagttc
      781 gcccatgcgg tattcttctt tcctgacgac tctaccgtca aggctgtact ctttgaagaa
      841 gccgtctttc ttgtcgtcct tgtaggtgcc ttcgcgcagc agctggtcgg tgtcgtaaaa
      901 atctttgtac agaccctgct tcatcccgtt cttgtcgacc cggttgatgc gttcttcttt
      961 ttgaaagtag ccgttgcggt agtaggtcac cgtgatcagg gtgccgtcgg cgcggtattc
     1021 gcgcgccagt ccgttttcca cgccgctggt gtagggaact actttgatga tcttgccgtc
     1081 ttcgttgtac gacttcaaca agccgttttt cacgctcatc gaatcggtgt actcgctttg
     1141 cagcttaccg ttgtcccagt aggtgcgttg caagccgttc ttgagtcctt ctttgtagtt
     1201 gtattcggcg gtgacgatgc cgctgtcgtt gtaaaagcgc cagaggctgt cgagtttgaa
     1261 gtcgacccgg ttgccttccg atttgatgcg gccgctttcg gagtagcttt tccagtagcc
     1321 gtcgggtttg ccgttgcgga gggtgccttc gctgcttacg ttcccgttgg ggtagtagta
     1381 cttcaccggt ccgtccttcg gcagttgcgc gagggagagg agcgggagca gtaacaggag
     1441 cagtgcgcgc ttaaacatga gggccggcta caataaaaat aacacagagg acgcagaggt
     1501 atgcacagag gggcacagag gtcgagttaa aatacaactg tgggctttgt aggtgtgatg
     1561 aaactaagtt acagtccttg ctggcgggag atctcgagca tgcgttggat ggggagcagg
     1621 gcttttttgc gcaggtcttc gtccatggtg agctcgggga cttcgtactt caaacaaaga
     1681 taaactttct ccagcgtgtt cagtttcatg tgcgggcagt cgttgcaggc acaggcgttg
     1741 ttcggcgggg ccgggatgaa ggtctttttc ggcgatgcct tcatcatctg gtgcaggatg
     1801 ccggtctccg tggcgacgat gaactcctgg tcggggctgt tcaccgtgta cttgagcagt
     1861 ccggtcgtgc tgccgatgta gtcggcgatc ttcagcaccg cctcttcgca ttccggatgg
     1921 gcgatgaact tcgcgttcgg gtgggcggct ttgaggcgga cgatcttttc gagcgagaag
     1981 atctcgtgca ccatgcaact gccgttccac agcaccatcg gccggccggt cttttccatg
     2041 atgtaccggc cgaggttctt gtcgggcgcg aagatgatcg gcgtatcctt gggtacgctg
     2101 ttcacgatgg cgatcgcgtt cgagctggtg cagatgatgt cgctcatcgc cttgatctcg
     2161 gcggtgcagt tgatgtagct gatcactacg tggccgggat gttctgcctt gaacttcgcg
     2221 aacgcatcgg gcggacacga atccgccagc gaacaacccg cgcggaggtc gggcagcacc
     2281 cctttcttgt cggggttcag gatcttggcc gtctccgcca tgaagtgcac cccggcaaag
     2341 aggatcatcg aagccgtggt cttctccgcc tgctgggcga ggccgaggct gtcgccgatg
     2401 taatcggcca cgtcctggat atcggcatcc tggtaatagt gggcgaggag gacggcattc
     2461 ttctccttct tcagccgggc gatctccgcg aacaggtcca aagtcggatc caccttccgg
     2521 tccagccagc ccttccggtc aacggaacgg atctcctcgg cggtaagcag tgtgtcggtc
     2581 atgatgggtg ggggcggaag ccccttgtac aaagatcggg aattagtgaa tagcgggttt
     2641 agcgaatagc caatagcgaa cagcgaatag tgggttttag tgaatagcgg ttttagcgaa
     2701 tagccaatag cgaatagcga atagtgggtt ctgggcgaat cttgcttgtt ttggttttga
     2761 gtaaacaagc tgaactcaat tcttcagaaa ttaccgtatc cggccaatgt tcaccaaata
     2821 ctattcgctg ttcgctgttc gctattggct attcgctaat cgctatttgc tttttcaatg
     2881 ctccattcaa caaaatgatc attcaaatgg tttttcaatc aggctttgcc gaataggatt
     2941 gtaccgccgg aaacaaaacc accgagcgaa tagcgaacag cgaatagcga ttagtgagtt
     3001 cgtggtcaat cttgattgtt ttggcttgga ttt
//
LOCUS       DAOTON010000282         3032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25368_length_3032_cov_2.617736, whole genome shotgun sequence.
ACCESSION   DAOTON010000282 DAOTON010000000
VERSION     DAOTON010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25368_length_3032_cov_2.617736"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="P5292_06505"
     CDS             complement(<1..348)
                     /locus_tag="P5292_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS38814.1"
                     /translation="MKYHSPFNIAVLAAGLGFFVDAFDLFLFNVYRIPSLKELGLSGV
                     ELTRAGEKLLSIQMAGMMLGGILTGMIADRRGRVTALFGSIVLYSLANIANAWVQDVD
                     SYALVRFLAGVGLA"
     gene            complement(599..1735)
                     /locus_tag="P5292_06510"
     CDS             complement(599..1735)
                     /locus_tag="P5292_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRS38815.1"
                     /translation="MQDARIYLDYNATCPVDPRVLERMLPYFNEKFGNASSRTHAFGW
                     EADEAVKLARRSVAGLLGCEPGELVFTSGATEAINLGIKGAAVAYQSKGRHFITCTTE
                     HRAVLDVFEHLERDGAEVTRLPVQPDGRLDPELLRSVLRNDTVLVAIMLANNESGVLQ
                     PIRELAELTHERGALFFCDTTQAIGKLQVAVNELGIDLCTVSAHKCYGPKGVGALFVR
                     RKNPRVTLLPQLDGGGHEHGLRSGTLNVPGIVGLGAAAELVVRELWDDNQRISLLRSR
                     LEQELITRCGAKVNGNTRHRLPNTTNLCFPGLRASELISACPQLAISTGSACSSALPE
                     PSHVLRAMGLSEADAYASIRISLGRMTTEEEVRRAVGMIASSCQ"
     gene            1948..>3032
                     /locus_tag="P5292_06515"
     CDS             1948..>3032
                     /locus_tag="P5292_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38816.1"
                     /translation="MPYNLQSLPKARTCFQAILTLVCFLQVSLSRGQVTVAGALGGVN
                     GAYTQLRLAFNAINTQLQGGANIVVTITGNTVETGTAQLIQSAAPWNSFTIIPSGGAA
                     RTISGNLALPLINLNGADRVKIDGLNAGGNSLTIVNNSTSALASTIQFSTDATLDTLV
                     RLTIQGTSRGAYNLIGDGATIKFGLAGTSTGNDNNYVGFCTIGPVGASLPHIPIKSHT
                     TSFSNDNITITDNLIFDYFATGSQSNGKGILAFTGSSTWTITNNRIYQTGTRTWPSSG
                     SLDGENIYSCIDIQSGSGYTITGNVLGYANSAGTGFTTLTSPLPLAIATNYASMLQPL
                     IVNQTTATPSVISNNQIGGFNLTSSRA"
BASE COUNT          684 a          868 c          816 g          664 t
ORIGIN      
        1 cgcgagtccg acgcccgcga ggaaccgcac cagcgcgtag ctgtccacat cctgcaccca
       61 ggcgttggcg atgttggcca gcgagtacag cacgatggat ccgaagagcg cggtgacccg
      121 cccgcgccgg tcggcgatca tgcccgtgag gatgccgccc agcatcatcc ccgccatctg
      181 gatcgacagc agcttctcgc ccgcgcgcgt cagttcaact ccgctgagtc caagttcctt
      241 cagcgaagga atgcggtaca cgttgaacag gaaaagatcg aacgcgtcca caaaaaagcc
      301 cagaccggcg gcgaggacgg cgatgttgaa gggagagtgg tatttcaagg gttattggtt
      361 tcgggtttcc agtttccagt ttcgtgtttc gagctgaatg gtcgatgtta atgtgcagtt
      421 cagatgatgc ctgaataaac tcttgatgtc gcaccatcca tccgtatgca ggcaaataac
      481 gatatggaag atcgcattgc caactcattt acaaggttga ctcaatcaaa gccggacact
      541 tgtaatgaaa accttcaaac aattgaacaa cccgaaactg gaaactggaa actcggaact
      601 actgacacga agaagcgatc atccccaccg ctcgacgcac ctcctcttcc gtcgtcatcc
      661 gaccgagcga gattcgtatg gaggcgtagg cgtcggcttc tgaaagcccc atcgcgcgga
      721 ggacgtgtga gggttctggt agggcggagg agcaggcact gccggtcgag atggcgagtt
      781 gcggacaggc gctgatcagt tcggaggcgc ggaggccggg aaagcagagg ttggtcgtgt
      841 tgggcaggcg atgccgggtg ttgccgttca ccttcgcgcc gcaacgtgtg atcagttcct
      901 gttccagacg cgatcgcaag agcgagatgc gttgattgtc atcccacagt tcccgtacga
      961 ccaactccgc cgcggcaccc agccccacga ttccgggaac attcagcgtg ccgctgcgca
     1021 acccgtgctc atgtccgcct ccgtccagtt gcggcaggag ggttacgcgc ggattttttc
     1081 ttcggacaaa caacgccccg acgcctttcg gtccgtagca cttatgcgct gatacggtac
     1141 acaggtcaat gccgagttcg ttcaccgcta cctgaagttt gccaatggcc tgtgtggtat
     1201 cacagaagaa gagcgcgccg cgttcgtggg taagttccgc cagttcccga atgggctgaa
     1261 ggacgccgga ctcgttgttc gccagcatga tcgcgaccag gaccgtatcg ttgcgcagta
     1321 cggaacggag cagttccgga tccagtcgtc cgtccggttg gaccggcaga cgggtgacct
     1381 cggctccatc ccgctcgaga tgttcgaaga cgtcgagtac cgcccggtgc tcggtggtac
     1441 aggtgatgaa gtgccgacct ttggattgat aagccaccgc tgctcctttt atgccgaggt
     1501 tgatcgcttc ggttgcgccc gaggtgaaaa caagttcacc gggctcgcat cccagcaagc
     1561 ccgcgaccga cctgcgggca agttttaccg cttcgtcggc ttcccagcca aaggcatggg
     1621 tgcggctgga tgcgttgccg aatttctcgt tgaaataggg gagcatccgt tccaggacac
     1681 ggggatcaac cgggcaggtg gcattgtaat caaggtagat ccgggcgtct tgcatcggct
     1741 tgggttttca aagaaacgga attctctaaa acatcgtttt ttcgcaaaat ttataggttc
     1801 acattaagaa ttggcccttg acattaaaaa caagccattt acctatggaa accttcaatt
     1861 aaccaaaaaa tggccttttc ttaacccgtt tttgggtacc tttccccact tttgcccggt
     1921 tgaaaatccg gctgtattgc tattgtaatg ccctataatc tccaatccct cccgaaggcc
     1981 cggacctgtt ttcaggcgat cctgacgctg gtctgcttcc tgcaggttag tctcagccgg
     2041 gggcaggtca ctgtagcagg ggctttgggg ggagtaaacg gcgcttatac ccagcttcgc
     2101 cttgctttca atgccatcaa tacccagttg cagggcgggg cgaacattgt tgtcaccatc
     2161 accggaaata cagtagaaac gggtaccgca cagctcattc agagcgccgc gccctggaac
     2221 agttttacca tcatcccctc gggaggtgca gcccgcacca tttctgggaa tcttgcactg
     2281 ccgctgatca atctcaacgg agccgatcgg gtcaagatcg atggcctgaa cgcaggtggg
     2341 aacagcctta cgatcgtcaa caacagcacc tccgccctgg catccaccat tcaattcagt
     2401 acggatgcaa cacttgatac cctggtccgc ctcacgatcc agggtacttc gcgcggagct
     2461 tataatttaa ttggagatgg cgcgacaatc aaatttggtt tagccggaac ttcgacaggc
     2521 aatgacaata attacgttgg cttttgcacg atcggaccgg tcggtgcaag tcttccccac
     2581 attccgatta agagtcacac tacctccttc agcaacgaca acattaccat taccgacaac
     2641 ctgatcttcg actacttcgc caccgggagc cagtcgaacg gaaagggaat cctggcgttc
     2701 accgggtcct cgacctggac cattaccaac aacaggatct atcagacggg aacgcgtacg
     2761 tggccttctt ccggcagcct tgatggtgaa aatatttaca gctgtataga tatccagtca
     2821 ggatcgggct ataccattac cggaaacgtc ttaggttatg caaatagcgc gggtaccggc
     2881 tttactactc tcacctctcc cttgccgctg gccatcgcca ccaattacgc cagcatgctg
     2941 cagccgctga tcgtcaacca gacgactgcc acgccttcgg tgatcagcaa caaccagatc
     3001 ggtggattca acctcacctc gagtcgtgcc aa
//
LOCUS       DAOTON010000283         3027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25424_length_3027_cov_3.170929, whole genome shotgun sequence.
ACCESSION   DAOTON010000283 DAOTON010000000
VERSION     DAOTON010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25424_length_3027_cov_3.170929"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3027)
                     /locus_tag="P5292_06520"
     CDS             complement(<1..>3027)
                     /locus_tag="P5292_06520"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS38817.1"
                     /translation="DNTVNNSAWFVITGISNGLATLTVSGIDNNPGLEMEVFTGLCGS
                     LTPVAGTCRSANGPAGSMTSNFAVTNGTTYYILVDGSSGNQEDFSIVCSATNNSIVGT
                     PQASFTTNVFRGCTPLTVQAFNQTVLYGGTNISYSWKLDTAADIPSSGADTTFQITSV
                     GPHTLLLTVCNDECGCTQFSRTIYVQELYPTYAFSPTNACIGTPVDFSASATIQPAFP
                     PTNPNVTLWQWNFGDPNSGVDNTATGQTVTHVFVGPGTSFTVTLTAFGTCGPETTTQT
                     INILPAPVVDAGSPQTICEGTTLLLNGTTTNATSPITWNWVGPGTLSCNDCPNPSITN
                     VPAGGPYSYSVDIVDNNGCVDDTTVEVTVNEKPVAFVGGTLDVCRYDTVAIDVIPIAG
                     VAPLTVQWTPSNVENDTLFSTFAYMGTSGQSFCAVLTDAIGCQSDPECQQININPQPS
                     INAASANLCATSPVLQNTFTVTGPSLGSTFSWSLSPNYSLITGAAADSSSITVTFPQG
                     VVASYSFTVVTEDNITGCRDTVSTTFSIAPGLNMSLSVPGSICEGSSANLSAGGASSY
                     AWTASPAYTFLDSTSANISASPAVTTVFTVTGTTGTCSQVLSDTLVILPRPIAAAAPI
                     PDFCGCSTINLNGTGSTVGMTYSWVSIAGLGITNANFLNATAYRCTSDVFTFRVTDPT
                     TGCFRDTAVSATHRPLPVASASVVPDLICNGVSTSIALNGNGSNTDPGTTYHWSSNNP
                     GVVISDTTLLNTSATVSTSTIFYLTVTDTLGCDTLATDTVNIYPLPVITANDPFICTS
                     DPSLLSTISVSGAGAGSNYNWTSVPSCTNPSSATGSSQTFDFAACGPGTYTFNITVTD
                     AVSNCITPLSQQVVVVSGVVLTTSSDTSICEGNSVTLAAAGANAYSWSNGNTTDTITV
                     SGLTAAGSPYDFLVTGTIGSCSATDTVQVTVNPIPATSPISGPTFICESDTAQIYSVT
                     PSGGNYTWTITGGTITNLAGNTITVNWDSAGTGSLAV"
BASE COUNT          625 a          744 c         1004 g          654 t
ORIGIN      
        1 accgcgaggc tgccggttcc ggcggaatcc caattcacgg tgatcgtgtt gccggccaga
       61 ttggtgatgg ttccgccggt gatggtccag gtataattgc cgccggatgg tgtgacactg
      121 taaatctgcg cggtgtcgct ttcacaaatg aaggtaggtc ccgagatcgg tgaagtggcc
      181 gggatcgggt tcacggtaac ctgtaccgta tcggtggcgc tgcagcttcc gatcgtaccg
      241 gttacgagaa agtcataggg ggaaccggca gcggtcaggc cgctcaccgt gattgtatcc
      301 gtggtgttcc cgttgctcca actgtatgca tttgctccgg ctgccgccag tgtcacgctg
      361 ttgccttcgc agatgctggt atcggaggat gttgtaagga cgaccccgct gacgaccacc
      421 acctgctggc tcagcggtgt tatgcaattg gataccgcgt ctgttaccgt gatgttgaag
      481 gtataggttc cgggcccgca ggcggcgaaa tcaaatgtct gggaacttcc ggtcgccgaa
      541 gatggattcg tacaggaagg aacgctggtc cagttgtagt tcgatccggc tcctgctccg
      601 ctcactgaaa tcgttgaaag gagggacggg tcggatgtgc agatgaacgg gtcgttcgct
      661 gtgatgaccg gaagcggata aatgttcacg gtatcagtgg ccagcgtatc gcaccccagg
      721 gtatcggtga cggtgagata gaagatggtc gaagtagaaa ccgttgccga agtgttgagt
      781 aaggtcgtat cgctgatcac cacgcccgga ttgttgctgc tccagtgata ggtcgttccg
      841 ggatcggtgt tgctaccgtt tccattcaac gcgatcgacg tactgacgcc gttgcagatc
      901 aggtctggaa caaccgatgc actggctaca ggcagtggtc gatgcgtggc gcttacagcc
      961 gtatcacgga agcaaccggt tgtgggatcg gttacgcgga aggtaaagac atcgctggtg
     1021 catcggtaag ccgttgcatt caggaagttg gcattggtaa tgccaagtcc ggcgatactg
     1081 acccacgaat aggtcatacc gacggtgctg ccggtaccgt tcaggttgat ggtgctacag
     1141 ccgcagaaat cggggatggg cgcggcagcg gcgatcggcc gcggaagaat gaccagggta
     1201 tccgataaca cctggctaca ggtgccggtc gttccggtaa cggtgaatac cgttgtgacg
     1261 gccggtgatg cggaaatatt ggcggaagtc gaatccagga acgtgtaagc ggggcttgca
     1321 gtccaggcat acgagctggc tccgccggca ctgaggttgg ctgagcttcc ttcgcagatc
     1381 gaaccgggaa ccgacaggct catgttcaat ccgggagcga tcgagaaggt ggtgctgacc
     1441 gtgtcccggc aaccggtgat gttgtcttcg gtaacgacgg taaaactgta agaagctact
     1501 acaccttgcg ggaaggtcac ggtgatcgac gaagagtcgg ccgccgcgcc ggtgatcagc
     1561 gagtagttgg gcgaaagcga ccaggaaaag gtggagccaa gcgaagggcc cgtgacggtg
     1621 aaggtgttct gcaacaccgg actcgtggcg cagaggttcg cggaggccgc gttgatcgac
     1681 ggctgcggat tgatgttgat ctgctggcac tcgggatccg attgacaacc gatcgcgtct
     1741 gtcaacaccg cgcaaaagct ctgtccgctg gttcccatgt aggcgaaggt gctgaagagc
     1801 gtatcattct cgacgttcga tggcgtccat tgaacggtta gcggtgctac gccggcgatc
     1861 gggatcacgt cgatggcgac cgtatcatac cggcagacgt cgagcgtgcc gccgacgaat
     1921 gccacgggct tttcattcac ggtgacttcc acggtcgtgt cgtctacaca accgttgtta
     1981 tcgacgatat cgacggagta ggagtaaggg ccaccggccg gcacattggt gattgaaggg
     2041 ttcggacaat cgttgcacga caaggtgccc ggtcccaccc aattccaggt gatcggacta
     2101 gtggcattgg tcgtggtgcc gttgagcagc aaggtggttc cttcacagat ggtctgcggt
     2161 gaaccggcat cgaccaccgg agcgggaagt atgttgatgg tttgcgtggt ggtttcaggc
     2221 ccgcaggtgc cgaaggcggt gagcgtcacc gtaaagctgg tgccgggtcc gacgaagacg
     2281 tgcgtcacgg tctggccggt agcggtattg tcgactcccg aattcggatc gccgaagttc
     2341 cattgccaaa gggttacgtt cgggttagtg ggtgggaaag ccggttggat ggtggcgctg
     2401 gcggagaaat cgaccggagt accaatgcag gcgttggttg gagaaaaggc ataggtagga
     2461 tacagttcct gcacgtagat ggtgcgggag aactgggtgc acccgcattc gtcgttgcaa
     2521 acagtcagga gtaacgtatg cggccctaca ctggtaattt gaaaagtggt gtcggcgccg
     2581 gacgacggaa tgtccgccgc cgtatcgagt ttccaactgt acgagatatt cgtgccgccg
     2641 taaaggacgg tctgattgaa ggcctgcacc gtgagcggtg tgcaaccacg gaagacgttg
     2701 gtcgtgaagg aagcttgcgg cgtaccgacg atcgagttat tggtggcgga acaaacgatg
     2761 ctgaagtctt cctggttgcc ggaactgccg tcaacgagga tgtagtaggt cgttccgttt
     2821 gtcaccgcaa aattagaagt catgctaccg gctggcccgt ttgccgatcg gcaggtaccg
     2881 gctaccggtg tgagcgagcc gcaaaggccg gtgaatactt ccatctccag gcccgggttg
     2941 ttgtcgattc cggagaccgt cagtgttgcc aggccgttgc ttataccggt tatgacaaac
     3001 caagcgctgt tgttgacggt attgtcg
//
LOCUS       DAOTON010000284         3022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25473_length_3022_cov_3.135490, whole genome shotgun sequence.
ACCESSION   DAOTON010000284 DAOTON010000000
VERSION     DAOTON010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25473_length_3022_cov_3.135490"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="P5292_06525"
     CDS             <1..378
                     /locus_tag="P5292_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRS38818.1"
                     /translation="FGLKGVSGVGISFGADRIYDVLEEIKGFPDFSTVSTRVLFANFG
                     EAEARYCLGLLQRLRNNGINAELYPDAAKMKKQLAYADARKIPYVALVGTEEMKNGQV
                     TLKDMQKGEQSTITFEELLHVLK"
     gene            397..1869
                     /gene="hutH"
                     /locus_tag="P5292_06530"
     CDS             397..1869
                     /gene="hutH"
                     /locus_tag="P5292_06530"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRS38819.1"
                     /translation="MYSISLTGLDFETIERILRERIPLKLSEDAKQRIREGRRYLDLK
                     LKEHKEPIYGINTGFGSLYNKSINEKDLGALQVNLMKSHACGTGDEVHPDIVRLMLLL
                     KIQGLSYGNSGVQLQTVERLIDFFNKDILPVVYQQGSLGASGDLAPLAHLCLPLIGEG
                     EVDYQGKRLPASKVLQSLKWKPIALQSKEGLALLNGTQFMSAHGVYAVLQAMHLASMA
                     DIIAAVSLEGFDGRIDPFDKSLHRIRPHQGQVDVAENFRSMLKGSQLIKRAKEHVQDP
                     YSFRCIPQVHGASRDAIQYAANVLHVEINAVTDNPTIFPEEDKILSGGNFHGQPLALA
                     LDFLAMALAELGSISERRTYLLISGQRSLPPFLVANPGLHSGFMIPQYSAASIVSQNK
                     QLCTPASVDSIVSSNGQEDHVSMGANAATKVVRVASNLQTILAIELFTAAQALEFRRP
                     QKSSPVIENFVTAFRKRVSFVEEDRVLYRDIESAREFLAW"
     gene            1988..>3022
                     /locus_tag="P5292_06535"
     CDS             1988..>3022
                     /locus_tag="P5292_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38820.1"
                     /translation="MQRRLLLTFLCFCSSFWASAQFHRMEGLRLDGFSVRQRDVSEEL
                     KQATKPELSGHPDLGLFPYNGPPCTDCFELIEKRSAESRYFVQKGSEQGHFFQQQSLG
                     PINYRDQQGYWREIDPRFKPVDVQPKQFAATQQPSPLMVDLGKPSAALFNDDVAIRFL
                     QGAYLYVRTATGVFQLPGGPNFHAITAGDDGIRVTDFYPDVDLLILTRQGEAEVDVVL
                     KRPIPIPDGVLIFGQGLSLPTGIQSRRPDNAPVNTFIHAPIRFSDASGSDRFGVESCF
                     AFDNTPGAKALPIESFYGDEKLEVAVPVSWLQDPATTYPVVIDPVVTSVNSIGAGLIA
                     GTRYGAVCWTN"
BASE COUNT          666 a          822 c          817 g          717 t
ORIGIN      
        1 ttcggactga aaggcgtttc cggtgtcggc atttcattcg gtgccgatcg tatctacgat
       61 gtgttggaag agatcaaggg gttcccggat ttcagcacgg tctctacccg ggtgctcttt
      121 gccaatttcg gcgaagcgga agcacggtac tgcctggggc ttttgcagcg actgcgcaac
      181 aacggcatca acgcggaact ctaccccgat gcggcgaaaa tgaaaaagca actggcttat
      241 gccgatgcgc ggaagatccc gtacgtcgcg ttggtgggaa cagaggagat gaagaacgga
      301 caggtgaccc tgaaggatat gcagaagggc gaacaatcaa ccatcacctt cgaggaattg
      361 ctgcacgttt taaaataagt tgaagaacaa atcatcatgt atagtatctc gttaactggc
      421 cttgattttg aaacgatcga acggattctc cgggaacgca ttccgctgaa gctttcagaa
      481 gatgcgaagc aacgcattcg cgaaggccgc cggtacctgg acctcaaatt gaaggaacac
      541 aaggagccga tctacggaat taacaccggt ttcgggtcac tctataacaa gtcgatcaac
      601 gagaaggacc tgggcgcctt gcaggtcaac ctgatgaaat cccatgcctg cggaacagga
      661 gacgaagtgc atccggatat cgttcgcttg atgttgttgc tcaaaatcca gggactgtct
      721 tacggcaatt ccggcgtgca actgcaaacg gtcgaacgcc tgatcgattt cttcaacaag
      781 gatatcctgc ccgtcgtcta tcagcaaggt tccctgggcg catccggcga cctggctccg
      841 ctggctcatc tttgcctgcc gttgatcgga gaaggggagg tggactatca ggggaagcgt
      901 ttgcctgctt cgaaagtact gcagtcgttg aagtggaaac ctatcgcgtt gcagtcgaag
      961 gaaggcctgg cactcttgaa cggaacccag ttcatgtcgg cccacggcgt gtatgcagtg
     1021 ttacaggcca tgcacctggc ctccatggcc gacatcatcg cggctgtttc gctcgaagga
     1081 ttcgacggtc gtatcgatcc gttcgataaa tccttgcatc gaatccgtcc gcatcagggg
     1141 caagtcgacg tggctgagaa tttccggagt atgctcaaag gttcccaact gatcaagcgg
     1201 gccaaggagc atgtgcaaga tccctattcg ttccgctgca tcccgcaggt ccacggcgct
     1261 tcgcgtgacg cgatccagta cgcggcgaat gtgttgcatg tagaaatcaa cgcggtgacc
     1321 gataatccga ccatcttccc tgaagaggac aagatcctct ccggcggcaa cttccatggt
     1381 caaccgctgg cactcgcact cgacttcctt gccatggcct tagcggagct cggttcgatc
     1441 tcggagcgcc gtacttatct gctcatctcc ggccaacgca gtctgccgcc tttcctggta
     1501 gccaaccccg ggctgcattc cggattcatg atcccccagt attcggctgc ttcgattgtc
     1561 agccagaata agcaactctg cacgcctgcc tcggtcgact cgatcgtatc ctccaacgga
     1621 caggaggatc atgtcagtat gggcgccaat gctgcaacga aagtcgtgcg cgtggcctcc
     1681 aaccttcaga ccatcctcgc gatcgagctc ttcactgccg cccaggcgct ggaattccga
     1741 cgcccgcaga agtcatcgcc cgtaatcgaa aacttcgtaa ccgccttccg gaagcgcgtg
     1801 tccttcgtgg aggaagaccg tgttctctac cgggatattg aatccgcccg ggaattcctg
     1861 gcctggtagc cttgtttcag cccaaattgt cagctttccc atgtcggaaa atcgggacaa
     1921 tcgccttgaa acagcctgtt tagtacggcg ccggaacctt aaatttgtat agcatcctcc
     1981 attcctcatg cagcgccgac tgctcctgac cttcctttgt ttctgcagtt ccttttgggc
     2041 ttccgctcaa tttcaccgga tggaaggtct gcgcctcgac ggattcagtg tccggcagcg
     2101 ggatgtatcg gaggaattga agcaagctac caaacccgaa ctcagtgggc atccggacct
     2161 tggtcttttc ccgtataacg gtcccccctg cacggattgc ttcgaactga ttgagaagcg
     2221 atcggcggaa agccggtact ttgtacaaaa aggttccgag caaggacatt tttttcagca
     2281 gcaatcgctg gggccgatca actaccgtga tcagcaaggc tactggcggg aaatcgatcc
     2341 gcgcttcaag ccggtggatg ttcagccgaa acaatttgcg gctacgcaac agccctcacc
     2401 tttgatggtg gatctcggga aaccttctgc agcattgttc aatgatgatg ttgcgattcg
     2461 atttctgcag ggcgcgtatt tgtatgtgcg aactgcaaca ggtgtctttc agcttcctgg
     2521 tggtccgaat tttcacgcca tcacagctgg ggacgatggt atccgtgtaa cggattttta
     2581 ccctgacgtt gatttgttga tcctcacgcg tcagggtgaa gcggaagtgg atgtggtatt
     2641 gaaacgaccg attccaattc cggacggggt gctcatcttc gggcagggat tgtcattgcc
     2701 aaccggaatt caatcccgtc gcccggacaa tgctccagtc aacactttta tccatgcacc
     2761 gatccgattt tccgatgcgt ccggttcgga tcgtttcggt gtggaaagtt gttttgcttt
     2821 tgacaacacg cctggagcca aagccttgcc gatcgaatcg ttttatggag atgaaaaact
     2881 ggaggtagcc gtaccggtca gttggctgca ggatcctgcc accacttacc cggtcgtgat
     2941 cgacccggtc gttaccagtg tgaattccat tggagcgggg cttatcgccg gtacgcgcta
     3001 tggtgcggtt tgctggacca at
//
LOCUS       DAOTON010000285         3022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25479_length_3022_cov_2.285811, whole genome shotgun sequence.
ACCESSION   DAOTON010000285 DAOTON010000000
VERSION     DAOTON010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25479_length_3022_cov_2.285811"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="P5292_06540"
     CDS             complement(<1..755)
                     /locus_tag="P5292_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38821.1"
                     /translation="MQQPAQPATSQVELVYDTASVRSKLQSTEDVPVAQLDSSHKDFP
                     RYLKLRQEVGEKQDETIRLFSSAIELNKKASQEKEEQVDLMQKAEATADANEKAKLLR
                     SAEKRKHDSEEHEAEAGKLMAQAQKLSDEIWENNQSLEALSLAIRKSGPARTSPATTP
                     GTSGTTTTDIRPATIPEMDETVRAESSVSIQQTGNDRAPVKAGPLTVPGIERMVVRSD
                     KPAYTDTAPIPVDQPLPEGLVFKVQIGAFRKPIP"
     gene            738..>970
                     /locus_tag="P5292_06545"
     CDS             738..>970
                     /locus_tag="P5292_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38822.1"
                     /translation="MSRLLHRARLLFIDGISVVCRLSGCIRRSVGNSIGWSNCRLRIV
                     CDPCPVCCSLNRIGIPLTSGLLGFRRTNGFLPFG"
     assembly_gap    971..980
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<981..2912)
                     /locus_tag="P5292_06550"
     CDS             complement(<981..2912)
                     /locus_tag="P5292_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38823.1"
                     /translation="MAEQAVEVRTEGVSSTANPTSQTTPAAGQPQAADEEQLATTSPN
                     QQEQNQTAATTSRVSPDSNDSGVVAVQQNVSEESQPTADFNRGHTVESPGKETGKPVV
                     GTNPEGSSVSEKNTADVLAAGTNPAITASDEHAANDKQSVIEPETTVNATTKPEGLPE
                     QKAQQSADERTVAIHQASEEQPKAEPNAMENRPKDQATSSGEPVVIAGQPTRESESRQ
                     QPTANQPSAGKSKDDKVAATTGTSQPTSSTAANATTIRSTDDAAQSVPVAVNADGAPV
                     SPQTTQNTDTTGTQTGSTDRSTSSTRLTMVPAMEGVSEETRLAYQNYKQRMEFSSRLD
                     ERSKELQTQVATMKNSPERDSLIQLSNSMSMESIRQWQSAQQQLQLAKADDPEIQTKA
                     ETAIATAKAAEPAAEPVVTSQELATVANPVRRDAVPTGESAARTGVSGNQSANTNRAD
                     RSEQPAGTQPATSGQVSAIKTDVEETNQLSVTPTTPSVEPASGEVRNDGQLATNASGN
                     QAADRITNDGEGQRSEVAANNPNEPRPVSGGDGAPATSTGEISRNESRQGAISPSAEQ
                     GTTTARQDRSDATPAAGRGDEQAVATKPSTIPSGAERAGQVVANPDQTPGEGPASAER
                     SNPVSKEAAPTVSGQETVTN"
BASE COUNT          498 a          791 c          869 g          854 t
ORIGIN      
        1 ggaatcggtt tgcggaaagc gccgatctgg actttgaaga cgagtccttc gggcaggggt
       61 tgatccaccg gtatcggtgc agtatcggtg tacgcgggtt tgtcgctgcg cacgaccatg
      121 cgttcgatgc ccggaacggt aagcgggcct gctttgaccg gcgcacgatc attgccggtc
      181 tgctgtattg aaacagatga ttcagcgcgt accgtctcat ccatttccgg aatagttgcc
      241 ggtcgaatgt cggtagtcgt tgttccggaa gttccaggcg tagtggcagg agaagtacgt
      301 gcaggacccg actttcggat cgccaggctg agcgcttcga gcgactggtt gttttcccag
      361 atctcgtcgg aaagcttttg cgcctgcgcc atcagtttgc cggcttccgc ttcgtgttct
      421 tccgaatcgt gtttacgctt ttcagcgctg cggagcagtt tggctttttc gttggcatcg
      481 gcggtagctt ccgctttttg catcagatcg acttgttctt ctttctcttg ggatgctttc
      541 ttgttcaact cgatagccga agaaaacaag cggatggttt cgtcctgctt ctctcccact
      601 tcctggcgca atttgaggta acgcggaaaa tccttgtggg aggagtccag ttgcgcgacc
      661 ggaacatctt ctgtggattg caatttactc cgaacggaag ccgtatcgta caccagttca
      721 acctgggagg ttgcgggttg agcaggttgc tgcatcgcgc ccggctgctg ttcattgacg
      781 gtatttcggt tgtctgccgc ttgagtggtt gtattcgtag gagtgttgga aacagcattg
      841 gctggagtaa ctgccggctc cgtattgtct gcgatccttg tccggtctgt tgcagcttga
      901 accgcattgg aattccgttg acctccggac tcctgggttt ccgacgaacg aacggattcc
      961 tgcctttcgg nnnnnnnnnn gttggtgacc gtttcctgac cggatacagt aggcgcagct
     1021 tctttcgaaa cagggttcga tcgctcagca gaagccggtc cttcacctgg tgtctgatcc
     1081 gggttggcaa cgacttgtcc cgcacgctct gcgccggatg gaatcgttga tggtttcgtg
     1141 gcaactgcct gctcatcgcc tcgtccagcc gcaggcgtag catcgctccg atcctgccgg
     1201 gcagtggtag taccttgttc agccgaaggc gagatcgcac cctgccttga ttcgttccgg
     1261 cttatttcgc cagtggatgt tgctggagcg ccatctcctc ccgacaccgg tcggggctcg
     1321 ttcgggttat ttgctgcaac ttcggaacgt tggccttcgc catcgttggt aatacggtca
     1381 gcagcttgat ttccggacgc gttggttgca agctgtccat cattccgcac ttctccggaa
     1441 gccggttcaa ccgacggggt cgttggtgta accgaaagtt gattcgtttc ctcgacatcg
     1501 gtttttatcg cgctaacttg tccggaagtg gcaggttgtg ttcccgccgg ttgctccgaa
     1561 cgatcagcac gattcgtatt cgccgactgg tttccggata cgccggtcct ggcagctgac
     1621 tcgccggtcg gcacagcgtc gcgtcgcacc gggtttgcaa cagtggcaag ctcctgactc
     1681 gttacgacgg gctccgctgc aggttctgca gcctttgccg ttgcgatggc agtttccgct
     1741 ttggtttgga tctccggatc atccgccttg gcaagttgta attgttgttg agccgattgc
     1801 cactgtcgga ttgattccat actcatggag ttggacaact ggatgaggct atcccgttcc
     1861 ggagagttct tcatcgttgc cacttgcgtc tgcagttctt ttgaacgttc gtcgagccgc
     1921 gaggagaatt ccatccgttg cttgtagttc tgataagcca gcctcgtttc ttcgcttaca
     1981 ccttccatag ccggaaccat cgtcagtcgg gttgatgagg tgctccgatc agtgctgccg
     2041 gtttgagttc cggtcgtgtc tgtgttttgc gtagtctggg gggaaaccgg agcaccgtct
     2101 gcgttgacag ccaccggtac cgattgcgca gcatcatccg tggaacgaat ggtcgttgcg
     2161 ttagctgcag tagaagatgt cggttgcgac gtcccggttg tcgctgcaac tttatcatct
     2221 tttgattttc cagcggaagg ctgattggcg gtcggttgtt gacggctctc tgattccctt
     2281 gtgggctgcc cggcgatgac aacaggctct cccgacgaag ttgcctgatc tttgggcctg
     2341 ttctccattg cattcggttc ggcctttggt tgttcttcgg acgcttgatg gatcgctaca
     2401 gtccgctcat cagccgattg ctgtgccttt tgttccggta atccttcagg cttagtggtt
     2461 gcatttacgg ttgtttcggg ttcaataacc gattgcttgt cgtttgctgc atgctcatcg
     2521 cttgcagtaa ttgccgggtt ggttccggca gcaaggacat cagcagtatt tttctctgaa
     2581 acagacgagc cctccggatt cgtaccgaca accggtttcc cagtctcttt tcccggcgat
     2641 tcaaccgtat gtccacggtt aaagtccgca gttggttgag attcttctga gacattttgc
     2701 tggacggcga cgacgccgga atcgtttgaa tcgggagata ccctggaagt tgtcgccgca
     2761 gtttgatttt gctcttgctg atttggagaa gttgtcgcca actgttcttc atccgcagct
     2821 tgcggctgtc cggcagccgg agttgtttga ctggtcgggt tggccgttga agagacacct
     2881 tccgtacgaa cttcaacagc ctgttcggcc atgggttcca acgagggaga tgtgactcct
     2941 tgagccgcac taggacttga tgcagtaacg gatgcctttt cactggttcc ggccaaggct
     3001 ctgtcaactg aagcggaaag tt
//
LOCUS       DAOTON010000286         3018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25510_length_3018_cov_3.177860, whole genome shotgun sequence.
ACCESSION   DAOTON010000286 DAOTON010000000
VERSION     DAOTON010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25510_length_3018_cov_3.177860"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="P5292_06555"
     CDS             <1..854
                     /locus_tag="P5292_06555"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRS38824.1"
                     /translation="YTRRTLRIFPTYYATIFIFYFLKLPSVKDNLVWFLTYTVNFRVM
                     QLEYWLPGSGHMWSLAIEEQFYIVFPFLVFFTPKKYLKGMLFAMIVTGIFSKIILWAL
                     NYSLFDISLFSLSSVDYLGIGALLAWLQRYTNVAHRLFEKRKLLLSSSLLILSIVLYL
                     RYHFANALFICVLVPMAFAYFAWTCILICSFGATGRAKKILEWPLFLFLGQISYGLYL
                     YHNLVPTLDILLIRKMATIDYLQWTSSALGQAFVHFVFTVVLAILSFRFFEQPISNLK
                     KYFEYRL"
     gene            1361..>3018
                     /locus_tag="P5292_06560"
     CDS             1361..>3018
                     /locus_tag="P5292_06560"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRS38825.1"
                     /translation="MHNITPILSALFALFTFGLQAQDTFYDINTIQEVRIYFGSSNWD
                     YQLDTAAAGSDGYIIADSVNVNGVNFPQAGVKYKGNSSYSASRVKNPFHIKLDKVIDQ
                     DYEGHEDIKLANGFSDPSMVREVSAYSICRQYMDAPRCNYARVYVNGAYWGLYTNSED
                     VGNSFAEDHYHSSYSSYFKCNPRNVMSGTGSSLIYLGTDSSLYYNYYELQSDYGWGDL
                     IRFCDSLTNHPTYAHTFVDVDRALWMLAYNNVLVNLDSYSGGFRQNYYLYYDHNRRWI
                     PTVWDVNMAYGSFTNTGAGGGGGGGGVTAMQNLTPVLHETDANWPLIRNLLSDPMYKR
                     MYLAHMRTLNNENFANADYKNLIGQLRTLIDSSVQADPNYLYTYTQYQSNLTTTVSGG
                     GGGPGGTCGVYELMDTRTTYLSGTTQLSAAPPAISAVAADPATAEYGSTVNITASVSN
                     ALSSAVYLGYRYKHSDVFVRVAMSDDGLHGDGAAGDGVYGATIPVNSLKMEYYLYAEN
                     ASAGMFSPERAEHEFYTLFATITMATSADVTVNEFLAGNSSGIT"
BASE COUNT          696 a          785 c          697 g          840 t
ORIGIN      
        1 tttatactag acgaacactt cgaatttttc cgacctatta tgcgacaatc tttattttct
       61 attttctaaa attaccttct gtcaaagata atcttgtttg gtttttaacc tacacggtca
      121 attttagagt gatgcaattg gagtattggc ttccaggttc cggtcatatg tggtcgttag
      181 cgatagaaga acaattctac atcgtctttc cttttcttgt gttttttact ccaaaaaaat
      241 atttgaaggg aatgcttttt gccatgattg taactggaat tttttcaaaa ataattcttt
      301 gggcactcaa ctactcgttg tttgatatct ccttgttttc tctaagctct gtcgactact
      361 tgggcatcgg tgctttgtta gcatggttac agcgatatac aaatgttgct caccgcttat
      421 tcgaaaaacg aaagttgcta ctttcatcat ctctccttat ccttagcatt gttctgtatt
      481 tgcgttatca ctttgcaaac gctcttttta tctgtgtgct tgtgccaatg gcgtttgctt
      541 actttgcttg gacctgtatt ctaatttgtt ctttcggagc taccggtcgg gctaaaaaga
      601 ttctcgaatg gcctttgttt ttgttcttgg gacaaatcag ctatggacta tacctttatc
      661 acaatttagt tccaacactc gacattcttc ttatccgaaa aatggcaacc atcgactact
      721 tacaatggac gagttctgcg ttgggacaag cattcgtaca ttttgttttt acggttgttt
      781 tggcaatcct ttcattccga ttcttcgaac aacccattag taatcttaag aagtattttg
      841 agtaccgctt gtaattcact tttaagcgtg tcataagttt ggcgttgacg caagatttaa
      901 ttgatgaatt tggtgtcata tttattatga agaagaggcg ttttttctaa aaattgactc
      961 caaaaaagcc tttttgagac atcaacatcc taccataatt tgcctttaaa gttgtagttt
     1021 ccctaccctc aataggtcag aaggaaagcc gggccattcg actggagaac gctgccgaca
     1081 atctcttctt atttgctctc ggcctgatct ttctgaccat cctcctgatc gcggcaactg
     1141 ttttctgatg tgccggatac tgaatggttc ggaccccaac agtcaagttg gaaatcgagc
     1201 gtttcaagtg attgcccgag ttgtccaccc ctgcggattc tcacacaggc catgacattc
     1261 gaatgacaat gcctgcgttg accgacaaac cggcgggatt catcgtcttt ttattccggc
     1321 caggttaaac ttttgttact tgccgtcgtc catttcccct atgcacaaca tcacgcccat
     1381 cctctccgcc ctgttcgccc tgttcacatt cggcctgcaa gcacaagaca ccttttacga
     1441 catcaacacc attcaggagg tccggattta cttcggttct tccaactggg actatcaact
     1501 ggacaccgcg gctgccggca gtgacggcta catcatagcc gattcggtaa acgtcaatgg
     1561 cgtcaacttt ccccaggcag gcgtcaagta caaaggcaat agctcttata gcgccagtcg
     1621 tgtcaagaat ccatttcata tcaaactgga caaggtcatt gaccaggact acgagggtca
     1681 cgaagacatc aagctcgcca atggtttttc cgatccatcg atggttcgcg aggtttccgc
     1741 gtattcgatc tgtcgccagt acatggatgc tccccgttgt aactatgcca gggtctacgt
     1801 gaacggagcc tactggggcc tctacacgaa ttcggaggat gtcggcaact cctttgccga
     1861 ggatcattac cattcctctt actcctcgta tttcaagtgc aacccgcgca acgtgatgag
     1921 cgggaccggt tccagcctga tctacctggg cacggattcg tcgctctatt acaattacta
     1981 cgaactgcaa tccgactatg gttggggcga tctcatccgc ttctgcgaca gcctgacgaa
     2041 tcatcccacc tatgcccaca cgttcgtcga tgtggaccgt gcactctgga tgctggccta
     2101 caacaacgtg ctggtcaacc tggattccta cagtggcggc tttcgccaga attactacct
     2161 ctactatgat cacaaccgcc ggtggattcc cacggtatgg gacgtcaaca tggcgtacgg
     2221 cagcttcacg aataccggtg cgggtggcgg cggcggcggt ggaggggtaa cggccatgca
     2281 gaacctcacc cccgtgttgc acgaaacgga tgccaactgg cccctgatca ggaacctgct
     2341 gtcggatccg atgtacaagc gcatgtacct ggcgcacatg cgtacgctca acaacgagaa
     2401 ctttgccaat gcggattaca aaaacctgat tggacagttg cgcacgctca ttgactcgtc
     2461 cgtgcaggct gatccgaatt acctctatac ctatacccaa taccaaagca acctgaccac
     2521 gacggtcagt ggaggaggtg gtgggccggg cggaacctgc ggggtgtacg agctcatgga
     2581 tacgcgtacc acgtacctga gtggaacgac gcaactcagc gctgctccgc cggccatcag
     2641 cgccgtggcg gcagaccctg ctacggcgga gtacggttcc acggtgaaca tcaccgcttc
     2701 cgtcagcaat gccctttcga gtgccgttta cctgggttac cggtacaagc atagtgatgt
     2761 attcgttcga gtggccatga gcgacgacgg actccacggc gatggcgctg cgggcgatgg
     2821 ggtgtatggc gcaacgattc cggtgaattc gctcaagatg gagtactacc tctacgcgga
     2881 gaatgcgagc gccggcatgt tctctccgga gcgcgccgag cacgagttct acacgctctt
     2941 tgccacgatc accatggcca ccagcgcgga cgtcacggtg aatgaattcc tggccggtaa
     3001 ctcaagcggg atcacgaa
//
LOCUS       DAOTON010000287         3013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25564_length_3013_cov_3.245436, whole genome shotgun sequence.
ACCESSION   DAOTON010000287 DAOTON010000000
VERSION     DAOTON010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25564_length_3013_cov_3.245436"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1725)
                     /locus_tag="P5292_06565"
     CDS             complement(172..1725)
                     /locus_tag="P5292_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38826.1"
                     /translation="MPIQSLEFLQKEVRPLYRCILRNARSRYGDNCFELVNIDGQDEV
                     VDFVHGKKLEILGKITIDCGREDNGHGGIFYQFFRKTSPVYKGKYEYRWRDGNEPVEL
                     SKPLIEGLFFYITRFNLQQEFSDFQRYQEELLGRFERDASAPRSNELESVLARQNRFW
                     KPLNFPKEWRDSEWFCFERTDIGICISCISFHAEEERIVVNMRTYYRDDNIGYHFHYK
                     GLASRDQAQEYVIIELFREDVPHTYASFVLRTGVYNEQGQHVSIGHHSYFAFRWAKYL
                     TKQVVLLRREQLPDLDFEPREIRREDAEYDQLIPLLIRRFLSNRDKNRLSMPGKLINR
                     LEGGTYSLKNWFDDYFRGREPDPRLAGTRGAYTIVYENHDEKAREPLVFAQLDIEMLE
                     PELGEEYSNTRLIYRYNKRTWSGRAHWVGSRSLQCRLVAEPEPIKGSLYLLLNLPKCE
                     TAEELHNHIKANPVYGIISGYSERRQEAVAFRFGMALTKQLERGGVKNPKLKEIVKEL
                     RKNPGMLRG"
     gene            1923..>3013
                     /locus_tag="P5292_06570"
     CDS             1923..>3013
                     /locus_tag="P5292_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRS38827.1"
                     /translation="MKPAPIKLGYVSSEGPSRPTSPIGFLSMLKAAGTANAEKALYYS
                     GDQHLLTIAPTGAGKAISAALPTLCEYPGQVIVNDPKGELYLRTAEKRRALGHRVVLL
                     DPFHIIPRARRDSFNLLDICYLRKEDMFAECQTLANNLSEGGSTDERYWDDNARNLIS
                     GLLGLLTHQSKAKRKRATVPDLLRTLFVEDLAFTIATQLDQDRNVPEHVRQAFSSYFA
                     APSDRTRPCIDNTAQGYLQIFKSPGILESLGKSTFDLQDIIDGKPITVYIVMPPDKMD
                     SHRVLMVQWLHALFTAITTRKKLPKEPTLFLLDETATLGKFPMLSTFIAICRGFGVRV
                     WTFWQELAQLHTFYPAQARTIIGNCGVIQL"
BASE COUNT          628 a          850 c          875 g          660 t
ORIGIN      
        1 tggcccaacg gcaaagagcg tgaagagcgc acctatcgcc tcggattact cgacggcgtc
       61 tggaaagttt tcgacgaagg cggcgcaccg atcgtaacga tgaccttccg caacggcgaa
      121 gagatcaaga ccgagcagga agagttgccc cggggcgagc cgggcgattg atcaaccacg
      181 cagcataccc ggatttttcc ggagctcttt tacgatctcc ttcagctttg gattcttcac
      241 cccgccgcgt tccagttgtt tcgtgagcgc catgccgaag cggaacgcaa ccgcttcctg
      301 tcttcgttcg ctgtaaccgc tgatgatccc atagacggga ttcgctttta tgtggttgtg
      361 cagctcttcc gccgtttcgc atttcggtaa gttcaacagc agatacaaac tgcctttgat
      421 cggttccggt tcggcgacga gccggcattg cagggagcgg gaacccaccc aatgcgcgcg
      481 tcccgaccag gtgcgtttgt tgtaccgata gatgagccgg gtgttgctgt actcctcgcc
      541 gagttcgggc tcgagcatct cgatgtcgag ttgcgcgaac accagcggtt cccgtgcctt
      601 ttcgtcgtgg ttctcgtaca cgatggtgta ggcgcctcgt gttccggcca gacgtgggtc
      661 cggttcgcgt ccgcggaagt aatcgtcgaa ccagttcttg agcgagtagg taccgccttc
      721 gagccggttg atgagtttac ccggcatgga gagccggttc ttgtcgcggt tggaaagaaa
      781 gcgccggatg agcagcggga tcagttgatc gtactcggca tcctcgcggc gaatttcccg
      841 gggttcaaag tcgagatcgg gcaactgttc gcggcgcagc agcacgactt gcttagtcag
      901 gtacttggcc cagcggaacg cgaagtagct gtggtggccg atgctgacgt gttgtccctg
      961 ttcgttgtaa acaccggtgc gcagcacgaa cgaagcgtag gtgtgcggca cgtcttcgcg
     1021 aaagagttcg atgatcacat attcctgcgc ctggtcgcgg gaggccagtc ctttgtagtg
     1081 gaagtgatag ccgatattat cgtcgcggta gtatgtgcgc atgttcacca cgattcgctc
     1141 ttcctctgcg tggaagctga tgcaactgat gcagatgccg atatcggtac gttcgaagca
     1201 gaaccattcg gagtcgcgcc attctttcgg gaagttgagc ggcttccaga aacggttttg
     1261 tcgggccagg acgctttcga gttcattcga gcgcggtgcc gaggcgtcgc gctcgaagcg
     1321 gccgagtaat tcttcctggt agcgctggaa atccgaaaac tcctgttgca ggttgaagcg
     1381 agtgatgtag aagaagaggc cctcaatcag gggtttggaa agttctaccg gctcgttacc
     1441 gtcgcgccac cggtactcgt acttgccctt gtacaccggc gaagtctttc gaaagaactg
     1501 atagaagatc ccgccatggc cgttgtcttc gcggccgcag tcgatggtga tcttgcccag
     1561 gatctccagc ttcttgccgt gcacaaagtc cacgacctcg tcctgtccgt cgatgttcac
     1621 caactcgaag caattgtcgc catagcggct acgggcgttg cgcaggatgc agcgatacag
     1681 cggccggacc tctttttgga ggaattcgag ggattggatg ggcatgttgg aaatttagat
     1741 gaaagataaa attaaatatg aaaaataaga atttgtaatt ggtaattagt aactagaaaa
     1801 aaggatgtta atgagtaaaa atgcggcatt atggacaatt tgaaggcatt gaatattttc
     1861 taaatgcagt aatgggatgc atttgacgtt ttccgcgttt cgcagtgtcg tatttcgtca
     1921 gcatgaaacc tgcgccaatc aagctcggct atgtctcatc ggagggcccg tctcgcccga
     1981 cctctccgat cggattcctt tccatgttga aagcggccgg cactgcgaac gccgaaaagg
     2041 cactctatta ttcgggcgac caacacctcc tcaccattgc gcccacggga gctggcaagg
     2101 cgatcagcgc cgcgctgccc acgctctgtg agtacccggg tcaggtcatc gtgaacgatc
     2161 cgaaaggaga gctttacctg cgaacagcgg agaagcgacg agccctgggc catcgcgtgg
     2221 tgctgctgga tccgtttcac atcattccca gggcgcggcg cgacagcttc aacctgctcg
     2281 acatctgtta cctgcgcaag gaggatatgt tcgccgagtg tcagacgctg gccaacaacc
     2341 tctcggaggg cggcagcacc gacgagcggt actgggacga caacgcccgg aacctgatca
     2401 gcggcttgct cggcctgctg acacaccaga gcaaggccaa gcggaagcgc gcgaccgtac
     2461 cggatctgct gcgcaccctg ttcgtcgaag atctggcgtt caccatcgcc acgcaactcg
     2521 accaggaccg gaatgtaccc gaacacgtgc ggcaggcgtt cagctcctat ttcgccgcgc
     2581 cctccgaccg tacgcgtccc tgcatcgaca acaccgcgca gggctacctg cagatcttca
     2641 agtcgcccgg tatcctggaa tcgctgggga agagcacctt cgacctgcag gacatcatcg
     2701 acgggaagcc gatcaccgtg tacatcgtca tgccgcccga caagatggac agtcaccggg
     2761 tgctgatggt ccaatggctg cacgccctgt tcaccgccat cacgacgcgc aagaaactgc
     2821 cgaaggagcc caccctgttc ctgctcgacg aaacggccac actcggaaag ttcccgatgt
     2881 tgtccacctt catcgccatc tgtcgcggct tcggagtgcg cgtgtggacc ttttggcagg
     2941 agctggcgca gttgcacacc ttctacccgg cacaggcgcg tacgatcatc ggcaactgcg
     3001 gtgtgatcca gct
//
LOCUS       DAOTON010000288         3006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25650_length_3006_cov_3.289055, whole genome shotgun sequence.
ACCESSION   DAOTON010000288 DAOTON010000000
VERSION     DAOTON010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25650_length_3006_cov_3.289055"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="P5292_06575"
     CDS             complement(<1..1255)
                     /locus_tag="P5292_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRS38828.1"
                     /translation="MIQNDNKQLPLQLMNILDDDSEFIPLMSQEDEDNMNQQEMPDVL
                     SILPLRNTVLFPGVVIPITVGRDKSINLIKDAYKGDKVIGVVAQRDVSIEDPKVTDLH
                     SIGTVANVMKMLRMPDGNTTIIIQGKRRFQLEQPVQTEPYIRCKVRSFPEPAVQGDDK
                     EFGASVSSLKDMALQIIQQSPNIPSDAAFAIRNIESPSFLINFISSNMNLDVVRKQEL
                     LEIADPKQRAMRVLEHLGKELQMLEMKNQIQSKVKTELDRQQREYFLNQQLRTIQEEL
                     GGSTPDREVMELRERAKQKKWSKEVNDVFQRECDRMSRMNPAAAEYSVLLNYLELMLD
                     LPWNEFTQDNFDLKFAQKVLDKDHYGLEKVKQRILEYLAVLKLKRDMKSPIMCLYGPP
                     GVGKTSLGKSIAKALGRKYVRMSLGG"
     gene            1520..2719
                     /locus_tag="P5292_06580"
     CDS             1520..2719
                     /locus_tag="P5292_06580"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRS38829.1"
                     /translation="MSTSSLSQRVQQLAESQTIGMAKLSRELASKGIDVINLSLGEPD
                     FVTPSHIREAAKKAIDDGFTFYPPIAGYAELRKAISAKFQRENQLDYKPDQVVVSTGA
                     KQAIANVVLCLVNPGDEVIIPLPYWVSYIEIVKLAEGKTVAVNTTVEADFKMTAEQLE
                     AAITPRTKLLIFSSPCNPTGSVYTREELEAIAKVLARHPQVYILSDEIYEHINFLDAH
                     QSIAQFESVRDRVIIVNGVSKGYAMTGWRIGYIGAPKFIADACDKMQGQFTSAASSIA
                     QKAAEAALNLDNRPTLDMRDAFLRRRDLVIEGLKKIPGLRLNQPKGAFYVFPDISSYF
                     GKSDGTTTIHNATDLCMYLLNTGHVSVVTGEAFGAPNCFRLSYAASDDKLKEAVRRIG
                     DALAKLR"
     gene            2766..>3006
                     /locus_tag="P5292_06585"
     CDS             2766..>3006
                     /locus_tag="P5292_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HRS38830.1"
                     /translation="MATRKTSAIDTLFRSMEKLNVLVIGDVMLDAYLWGNVDRISPEA
                     PVPIVSIRKKENRLGGAANVAINVRALGATPLLCSV"
BASE COUNT          695 a          830 c          769 g          712 t
ORIGIN      
        1 ggccgccaag cgacatgcga acatacttcc ggcccaaggc tttggcaatc gattttccga
       61 gcgatgtttt cccgacaccc ggagggccgt acaggcacat gatcggcgac ttcatgtcgc
      121 gtttcaactt caggacggcc aggtactcca gaatgcgctg ctttaccttc tccagtccgt
      181 agtggtcctt gtcgagtact ttttgagcga acttcagatc aaaattatcc tgcgtgaatt
      241 cgttccacgg aaggtcgagc atcaactcga gataattcag cagaaccgag tattccgcag
      301 cagccggatt catgcgcgac atcctgtcgc attcgcgctg gaatacatcg ttcacttctt
      361 tgctccattt cttctgcttg gcccgttcgc gaagctccat cacttcacgg tcgggtgtgg
      421 agcctcccag ttcttcctgg atggtccgta actgctggtt caggaaatat tcccgctgtt
      481 gccggtccag ctcggtcttt accttgctct ggatctgatt tttcatttcc agcatttgca
      541 gctctttgcc caaatgttcc aatacgcgca tggcgcgttg cttcgggtcg gcgatctcca
      601 gcaattcctg tttccggacc acatcgaggt tcatgttcga cgagatgaaa ttgatcagga
      661 agctcggact ttcgatgttc cggatggcga aggccgcatc ggacggaatg ttcggcgatt
      721 gctggatgat ctggagcgcc atgtctttca aagaagaaac ggaagcaccg aattccttgt
      781 catccccttg aaccgccggt tcggggaagg aacggacttt gcaacggatg tagggttcag
      841 tctgaacggg ttgctccaat tggaaacgtc gcttaccctg aatgataatg gtggtatttc
      901 catccggcat acgcagcatc ttcatcacat tggcaaccgt tccaatgctg tgcagatcgg
      961 tgaccttcgg atcctcgata ctcacgtcac gttgcgccac cacgccgatc accttatcgc
     1021 ccttgtaagc gtctttgatt aggttgatcg acttatcacg gcccacggtg atcggaatga
     1081 ccacgccggg aaacaacacc gtattccgga gcggcagaat ggatagcaca tccggcatct
     1141 cctgctggtt catgttgtcc tcgtcctcct gggacatgag cggtatgaac tcagagtcgt
     1201 cgtcgagtat attcatgagt tgcagcggca gttgcttgtt gtcattttgg atcataatgg
     1261 agaataatgc gtcacagtgg cagtgcattc caccacaaag gcacgccatg ggacgggtcg
     1321 aattccgtgc cagcaagaat acgctgacaa aatgagattt tcgtccaaaa aatgcgaatt
     1381 taatgacctc cagaaacgtt gtaactgact gtattacagt acacaaacca ttctgacgta
     1441 ttatttgagc aaagtgtgaa acaaaaggcc ttcgccgggg gtaatgattc ctattatctg
     1501 cgtaggtttg caagcttcta tgagtacctc ttctttatcc cagagggttc agcaactggc
     1561 tgaaagtcag acgatcggaa tggccaagtt gagccgggaa ctcgcttcca agggtatcga
     1621 cgtcatcaac ctcagcttag gtgaaccgga cttcgtgacc ccttcacata tccgggaagc
     1681 ggccaagaaa gccatcgacg atggcttcac attctatcct cctatcgctg gctacgcgga
     1741 attgaggaaa gccatctcgg cgaaattcca acgggaaaac cagcttgact acaaacccga
     1801 ccaggtcgtg gtttccaccg gcgcaaagca ggccattgcc aatgtggtgc tctgcctggt
     1861 taatcccggc gacgaagtca ttatcccatt accgtattgg gtgagttata tcgagatcgt
     1921 caaattagcc gaaggaaaaa cggtggcggt gaacacgacg gttgaggcag acttcaagat
     1981 gacggcggag caactggaag ccgctatcac accgcgtacc aagttgctca tcttctcctc
     2041 tccgtgcaat ccgaccggat cggtctatac ccgggaagag ttggaagcca ttgccaaggt
     2101 actcgcgcgt catccgcagg tgtacattct gtctgacgag atctacgaac atattaattt
     2161 tctcgatgcc catcagagca tcgcccaatt cgagtctgtc cgtgaccggg tgattatcgt
     2221 gaatggcgtt tccaaaggat acgccatgac cggctggagg atcggctaca tcggcgcacc
     2281 gaagttcatc gccgatgcct gcgacaagat gcagggacaa ttcacgtcag ccgcatcttc
     2341 catcgcgcaa aaagccgcgg aagctgcatt gaatctcgac aaccgaccga cactcgacat
     2401 gcgtgacgcg tttctacgcc gtcgcgacct tgtcatagaa gggctgaaaa aaataccagg
     2461 gctgcggctg aatcagccga aaggcgcgtt ctacgtattt cccgacatca gcagttattt
     2521 cggcaaatcg gatggcacca ccaccattca caacgcgacc gacctctgca tgtacctgct
     2581 gaacacggga catgtcagcg tcgtgaccgg cgaagcattc ggcgctccga attgtttccg
     2641 cctttcgtat gccgcatccg acgacaagct gaaggaagca gtgcgccgca tcggcgacgc
     2701 gcttgcgaaa cttcgctaat ttccgggaga cagtccatcc tgtcaccggt aacgaatcat
     2761 ttgtcatggc tacccgaaaa acctccgcca tcgatacgct tttccggtcc atggaaaagc
     2821 tcaacgtgct cgtgatcggc gacgtgatgt tggacgcgta cctctggggc aacgtggacc
     2881 ggatctcgcc ggaagcacct gttccgatcg tatccatccg caagaaggaa aaccggcttg
     2941 gcggcgccgc caacgtggcg atcaacgtac gcgctcttgg tgctacgccc ctgctctgct
     3001 cggtca
//
LOCUS       DAOTON010000289         3000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25722_length_3000_cov_3.213243, whole genome shotgun sequence.
ACCESSION   DAOTON010000289 DAOTON010000000
VERSION     DAOTON010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25722_length_3000_cov_3.213243"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="P5292_06590"
     CDS             complement(<1..1006)
                     /locus_tag="P5292_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRS38831.1"
                     /translation="MNLNNFTIKSQEALQQAQQIAAGLEQQSIEPAHIFQGMLEVDEN
                     VIPFILKKIGIKPDHLQNATDTLVRSYPKVSGARQYLSDESNKLVQKAISTAKTLGDE
                     YVSLEHLFVALLGIRDRMSGILKDAGITEKETMAAIRELRKGNKVTSASAEETYNSLS
                     KYARNLNELARQGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGVGKTAIAEGLA
                     HRIINGDVPENLKSKQIYSLDMGALVAGAKYKGEFEERLKSVVKEVISAEGEIVLFID
                     EIHTLVGAGGGEGAMDAANILKPALARGELRAIGATTLAEYQKYIEKDKALERRFQKV
                     M"
     gene            1210..1611
                     /locus_tag="P5292_06595"
     CDS             1210..1611
                     /locus_tag="P5292_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38832.1"
                     /translation="MDIRIAPDLTIGSIQEQFQKRFPFLKIEFFKVKSGATIKPENRV
                     DAHKRFSDLNSDPTAGQIHLTGLTTVRELEEQIRSQFGIHAEIFRMSGKVWLRTTTTD
                     TWTLDEQNAEARESGANEQDQGERPDYHEQD"
     gene            complement(1618..>3000)
                     /locus_tag="P5292_06600"
     CDS             complement(1618..>3000)
                     /locus_tag="P5292_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRS38833.1"
                     /translation="TIYISVNDLSLDTYYLRVYHKLSGSGLTGQFSLCIYATPPPPGN
                     DECSSAVYLSVNPTCIGIAGTSTLAATPSTGIPTCSVSGTIPDDDVWYSFKAQNPVCY
                     ITVQGGSGFNPVVQLFSGSCGNLQSLQCANFTGSGQSETITASGLTIGATYFVRVYHA
                     ASGSGSGVFSICVYSPPPSCTGGYIPANATQNVPASGTQLRWNKASNTNSYKVYFDMV
                     NPPVAMLAQVTDTFVSTGALTLGASYYWMAVPVNASGDATGCSPVVFATDPTGVPMRL
                     STYLQGYYLSGGTMIAAVDAGVLDTIADTLSVSVAASTPPYQIVGTSKGLLSTNGIAY
                     FDIPQPCFGLGQYYLVLNHRNHLETWSALPVQFDSEDSLYVLSDSPSRILGGNVIEVE
                     PGYWAMFAGDINKDGLIESTDYSAVENAAIQFLFGYEPTDLNGDGLVEAADYSLVENN
                     SLLFLFTITP"
BASE COUNT          781 a          760 c          730 g          729 t
ORIGIN      
        1 ccatcacttt ctggaatctg cgctccagcg ccttatcctt ttcgatgtac ttctgatatt
       61 cggcaagggt agttgctccg atggctctta gctccccgcg cgcaagcgca ggcttgagga
      121 tgttggcggc atccatggcg ccttcgccac cgcctgctcc gaccagcgta tggatctcat
      181 cgatgaacag gacgatttct ccctcggcac tgatgacttc ttttactacc gacttcaggc
      241 gttcttcgaa ttcaccctta tattttgcgc ctgccaccag tgcgcccatg tcaagcgagt
      301 agatctgctt gctcttcagg ttctccggaa catctccgtt gattatccgg tgggcgaggc
      361 cttcagcaat agctgtttta cctacaccag gttcgccgat caggataggg ttgtttttcg
      421 tgcgtcggga aaggatctgc aagactcggc ggatctcttc atcccgcccg atgaccggat
      481 caagctttcc ctgacgcgcc aattcattga ggttccgggc gtatttactc agagaattat
      541 acgtttcttc tgcactggcg gaggtcacct tgttgccttt ccgaagttcc cgaatggcgg
      601 ccatggtctc cttttcggtt atacccgcat ctttcaaaat accagacatc cgatcacgga
      661 ttcccagcag cgcgacaaac aggtgctcca gggaaacgta ctcatcaccc agggtcttgg
      721 cagttgaaat ggctttctgt acaagcttat tgctttcatc agaaagatat tgacgcgctc
      781 cactgacttt cgggtacgaa cgaaccagcg tatccgtagc attttgaaga tggtccggct
      841 ttatgccgat tttctttaga atgaacggga tcacgttttc gtccacttcc agcattccct
      901 gaaagatgtg agccggttca atggactgtt gttccagacc tgcagcgatt tgctgagcct
      961 gttgcaaagc ctcctgtgac ttgatggtga aattattcag attcatagct gtcttattca
     1021 aactcgctgc atcaatttgg gagccaacgc aagatcatgt caaatctact gcaacattgt
     1081 cagggaaagc aaagttcgga aagtcagctt gtcaggatcg ctgatttaca tctgaactta
     1141 ggctgataac ggtcattcaa aaatggtcgg attctggcgt acttcgatat tcagttaata
     1201 caagatagca tggatatccg catcgcacca gatttgacca tcggcagtat tcaagaacag
     1261 tttcaaaaac ggtttccatt cctgaagatc gaattcttca aggtcaaaag cggagcaact
     1321 atcaaaccgg agaacagggt ggatgcacac aaacggttta gcgatttgaa ttcagatccg
     1381 accgctggtc agattcacct gaccggcctg acaaccgtgc gggaattgga agagcagatc
     1441 cgtagtcagt tcgggatcca tgcagaaatc ttcagaatgt ccgggaaagt ctggttgagg
     1501 accacgacaa ccgatacctg gaccctggat gaacaaaacg ctgaagccag agaatccggc
     1561 gcgaacgaac aggatcaggg agaacgccct gattaccacg aacaggattg atgcgatcta
     1621 cggagtgatg gtgaacaaga acaagaggct gttattctca accaaagaat aatccgcagc
     1681 ctctaccaat ccgtccccat tcagatccgt cggctcataa ccgaaaagga attggatcgc
     1741 ggcgttctcg actgcactat agtcggtgga ttcaatcagc ccgtctttat tgatatcacc
     1801 ggcgaacatc gcccaatatc ctggttcgac ttcgattaca ttgccaccaa gtatccggga
     1861 gggagaatcc gatagaacat aaagtgaatc ttccgaatcg aattgaacag gaagtgcact
     1921 ccaggtttcg aggtgattcc tgtgattcaa caccaaataa tactgaccta agccaaaaca
     1981 gggctgcggg atatcgaaat aggcaatacc attggtgctt agcagtcctt tgctggtacc
     2041 gacgatctga taaggcggtg tgcttgcagc gacacttacc gataatgtat cggcgatcgt
     2101 atccaggaca cctgcgtcaa ccgccgcgat catggttcct ccggacaggt aatagccttg
     2161 aaggtaagtc gacaaccgca tcggaacacc ggtagggtcc gtggcaaata caaccgggct
     2221 gcaaccggtt gcatcaccgg aggcattgac cggaacagcc atccaataat aggaggctcc
     2281 cagtgttaat gcaccggtcg atacaaatgt atccgtcacc tgagccaaca tagctaccgg
     2341 agggttgacc atgtcgaaat agaccttata cgagttggtg ttactcgcct tattccaacg
     2401 taactgtgta ccggatgccg ggacattttg ggtggcattc gcagggatgt aacctccggt
     2461 gcaggaaggt ggaggagagt aaacgcaaat tgaaaacact ccgcttcccg agccactggc
     2521 cgcgtggtag accctgacaa aataggtcgc cccaattgtc aaaccggatg cagtgatcgt
     2581 ctcactttga ccggaacctg taaaattggc gcattgtaag gactgaagat tgccacatga
     2641 tcctgaaaac agttgaacaa ccggattgaa tcccgaaccg ccctggacag tgatgtaaca
     2701 aaccggattc tgcgccttga aggaatacca aacgtcatca tccggaatgg ttccggaaac
     2761 actgcacgtc ggaattccgg tacttggagt ggctgccagc gtagaggtgc ccgctattcc
     2821 gatacaagta ggattgactg ataagtaaac tgcactgctg cattcatcgt tgcccggtgg
     2881 cggcggagtt gcatagatgc acaaactaaa ctgccccgtc agaccggaac cgcttagttt
     2941 atgatagaca cgcaaataat aagtatctaa acttaaatca tttactgaaa tataaattgt
//
LOCUS       DAOTON010000290         2998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25741_length_2998_cov_3.293918, whole genome shotgun sequence.
ACCESSION   DAOTON010000290 DAOTON010000000
VERSION     DAOTON010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25741_length_2998_cov_3.293918"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="P5292_06605"
     CDS             <1..1004
                     /locus_tag="P5292_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRS38834.1"
                     /translation="ESSRLELDELLSDKSATSAEDTSYKLKFNPRLRADLDVEVGQLT
                     FRRFKAEDIAGRILLDHEVITGRQLRFRSMNGTCTMDATIDAARDDSVYMRCVADIKR
                     IDIRELFYELENFDQETMTDKNVKGLVSASVQFSSTWTSGLVNDPRKTRSAIELTIEN
                     GELNDFAPLQPVAKYIHLSDLNRIRFSTLRNTITIERRRINIPQMEVQSSALNLSLSG
                     VHDFDNIVDYRLQLLLSDVLGRKVKQQNSEFGEVEDDGLGRTRLFLCMKGPVDDPKIG
                     YDRKGAGEKVRSDLAREKQTIKSMLKEEFGLFRKDTAKAPPARKKEELQVDWDNNGD"
     gene            1092..2240
                     /locus_tag="P5292_06610"
     CDS             1092..2240
                     /locus_tag="P5292_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRS38835.1"
                     /translation="MNIYTQKQRWKLLLLLAALLIGAASLWYTNKLVNKLSEEEYKRI
                     QLWAEATRRLADVTEMNTDINFLSSVISNNNSIPVIWADEEFKVISYRNLDSLKALDS
                     TYLNRQALIMRTEHDPIEIRIGQNLKQYILYRDSDLLIRLRYYPYFQLGVIALFLFVS
                     YLAFSSSRNAEQNQVWVGMAKETAHQLGTPLSSLLAWLEFLKIKGTSSEYTNEIEKDI
                     QRLQTITDRFSKIGSAPSLKKENVRTVMQHSIDYIRTRSSDKVTFSLEAPAYDIQAPM
                     NVPLFEWVVENILKNALDAMEGNGRILVMITDQQQFVYIDISDTGKGVPKSSYKTIFK
                     PGFTTKNRGWGLGLSLSKRIIEEYHDGQIFVKSSEIGKGTTFRIVLKK"
     gene            2240..>2998
                     /gene="hemW"
                     /locus_tag="P5292_06615"
     CDS             2240..>2998
                     /gene="hemW"
                     /locus_tag="P5292_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRS38836.1"
                     /translation="MSGLYLHIPFCRQACHYCDFHFSTVQKSLPDLMGAMRQELRMRA
                     AELPGPTIDTVYFGGGTPSLLPIGELEATFRVIRELFHVSETAEITLEANPDDLDPAA
                     LEAWRSLGINRLSVGIQSFHEADLRYMNRVHTAEEALSAVQRARAASFELLTIDLIYG
                     TPGMDDEKWLENLEVLRGLGLDHFSAYSLTVEPRTALDKLIRQRVLHDTDEEQAARQF
                     GLLLDWAEANGFEHYEISNFARPGRYSQHNTAYWF"
BASE COUNT          725 a          797 c          827 g          649 t
ORIGIN      
        1 tggaaagctc gcgtctggaa ttggatgaac tcttgtccga caagagtgcc acgagtgcgg
       61 aggataccag ttacaaattg aaattcaatc cacgtttgcg cgcggacctc gacgttgaag
      121 tcggtcaact tacgttcagg agattcaaag cggaggacat tgccggcagg atcctgcttg
      181 atcacgaagt aatcaccggg cgacagctgc gtttccgttc gatgaacgga acgtgtacga
      241 tggacgctac gattgacgcg gcacgcgacg acagtgtgta catgcgctgt gtggcggata
      301 tcaagcgcat cgacattcgg gaattgttct acgagctgga aaacttcgat caggaaacga
      361 tgacggataa gaacgtcaaa ggtctggtca gcgcgtcggt acagttcagt tccacctgga
      421 ccagcgggct ggtgaacgat ccgcgcaaga cccgatccgc catcgaactg accatcgaga
      481 acggagagct caatgacttc gcgcccttac aaccggttgc gaagtacatt cacttatccg
      541 atctgaaccg catccgattt tccacactcc gcaataccat caccatcgag cgcaggcgga
      601 tcaacatccc gcagatggaa gtgcaatcca gcgccctgaa cctttccctg agcggtgtgc
      661 atgacttcga caacatcgtt gactaccgcc ttcagctcct tttatcggat gtattgggtc
      721 gaaaggtcaa gcaacagaat tcggaattcg gagaggtcga agacgacggc ctgggacgta
      781 cccgcctttt tctttgcatg aaaggtccgg tcgacgatcc caagatcgga tacgaccgga
      841 aaggtgcagg ggagaaggtg cggtctgacc tggcccgtga aaagcagacc atcaagtcga
      901 tgctgaaaga ggagttcggg ctgttccgca aggacaccgc aaaggccccg cctgcaagga
      961 aaaaggagga attgcaggtg gattgggata ataacgggga ttgatttccc gttttctgaa
     1021 attctgccat ctttgccaac ctgtccgaat ggccttttag gcctgaaaac ggggaaaaac
     1081 cgtcctccac ggtgaacatt tacacccaaa aacaacgttg gaaattgctg ctgttgctgg
     1141 ccgcgctact catcggcgcc gcctcgctgt ggtataccaa caagttggtc aataaactgt
     1201 cggaagagga atacaagcgt atccagctct gggcggaggc gacccgtcgg ctcgccgatg
     1261 tgacggagat gaatacggac atcaacttcc tctcctccgt tatcagcaac aacaattcga
     1321 tcccggtcat ctgggccgac gaggagttca aggtcatttc ataccggaat ctcgactcac
     1381 tcaaagcact cgactcgact tacctcaacc ggcaagccct gatcatgcgc acggagcatg
     1441 atccgattga gatcaggatc ggccagaatc tgaagcaata cattttgtac cgtgattcgg
     1501 atctcctgat ccgattacgt tattatcctt acttccagtt gggtgtcatc gcgcttttcc
     1561 tgttcgtttc gtacctggct ttctccagtt cccgaaacgc ggaacaaaac caggtgtggg
     1621 tcgggatggc gaaggagacc gcgcatcagc ttggtacgcc cctctcgtcc ctgctggcgt
     1681 ggctggagtt cctcaagata aaagggactt cttcggagta tacgaatgaa atagaaaagg
     1741 acattcaacg gctgcaaacc attaccgacc gcttttcgaa gatcggctcc gctccgagtc
     1801 tcaagaagga aaatgtgcgc acggtcatgc agcacagcat cgactacata cggacacgtt
     1861 cgtcggacaa ggtcaccttc tcgctcgaag cgccggccta cgacatccag gcgccgatga
     1921 atgttccctt gttcgagtgg gtggtggaga atattctcaa gaatgccctg gacgccatgg
     1981 agggcaacgg acgaatcctg gtgatgatca ccgaccagca acagttcgtc tacatcgaca
     2041 tttccgacac gggcaaaggt gttcccaaat cgagctacaa gactattttc aaaccagggt
     2101 tcacgaccaa gaaccgcggt tgggggttgg gcttatccct gagtaagcgg atcatcgagg
     2161 aataccacga cggacagatt ttcgtgaaga gttcggagat cggaaaggga acgactttcc
     2221 ggatcgtgct gaagaagtga tgtccggtct ctatctgcat attcccttct gccggcaggc
     2281 ctgccattat tgcgatttcc atttcagtac ggttcagaag tctcttcccg acctgatggg
     2341 cgcgatgcga caggagttgc ggatgcgtgc ggccgaactt ccaggaccaa cgatcgatac
     2401 cgtttatttc ggcggaggta cgccctccct gcttccaatc ggtgagctcg aagcgacgtt
     2461 tcgcgttatc cgggaattgt tccatgtgtc cgagacggcg gagatcaccc tggaggccaa
     2521 cccggatgat cttgatcctg ctgcactgga agcctggcga tccttgggga tcaaccggct
     2581 ttcggtcggg atacagagtt ttcacgaagc cgatctgcgc tacatgaaca gggtccacac
     2641 ggcggaggaa gccttgtcag cggttcaacg ggcacgcgcc gcgagttttg agttgctgac
     2701 gatcgatctc atctacggca cgccaggaat ggatgatgaa aagtggctgg agaacctgga
     2761 agtgttgcgc ggactcgggc tggatcattt ttccgcctac tcgctgacgg tagaaccccg
     2821 cacggccctg gacaaattga tccggcaacg agtactgcac gatacggatg aagagcaggc
     2881 ggcacgccag ttcggactac tactggattg ggcggaggcc aacgggttcg aacactacga
     2941 gatctccaac ttcgctcgac ccggaaggta ctcgcagcac aataccgcgt attggttc
//
LOCUS       DAOTON010000291         2993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25794_length_2993_cov_2.577604, whole genome shotgun sequence.
ACCESSION   DAOTON010000291 DAOTON010000000
VERSION     DAOTON010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25794_length_2993_cov_2.577604"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..2162
                     /locus_tag="P5292_06620"
     CDS             177..2162
                     /locus_tag="P5292_06620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS38837.1"
                     /translation="MKLSVVIVNYNVQYFLEQCLRSVEIACKRVSSEVFVVDNNSVDG
                     SVEMVQEKFPSVRLIANKENLGFSKANNQAIRIASGEYVLLLNPDTVVEEDTFEKIVR
                     FMDEHPDAGGLGVKMIDGSGRYLPESKRGLPTPAVAFYKIFGLSALFPRSTRFGRYHL
                     GFLDKNETHVVDVLAGAFMLLRQAALTKCGLLDEDFFMYGEDIDLSYRIQKAGFKNYY
                     FPGTQIIHYKGESTKKSSVNYVFVFYNAMIIFAKKHFSPQNASLFSFFIRIAIYLRAG
                     AAVLTRAFRNLLLPALDFGFLFAGLYFLQAYWGTNMNVRYPELFLQAAVPTYILCWIV
                     CIYFSGGYDVPIRIAKVVRGVFTGTLIILVGYALLPEHYRFSRALILLGAAWATAAAV
                     VIRLVLNVLWKKRYELANDRPKRLIIVGSEEEGSRVLSLLKLTGTNHNFIGFVRPEEI
                     TRSHPGKSQELNTYRLGDLSQLTSIVEVFAIDEVIFCARDLASNRIIALMAGMAGKAI
                     EFKIAPPDSLFIIGSNSIDDPGELYVIDINSIGKKENRRNKRFLDITFSIALLLVSPI
                     LMWLQQRPWTFFLNLFKVLLGSLSLVGYNLAASGLDELPRIRPGILHPGDQLRDLTTD
                     SSTLQRLNTLYAKDYQTDTDLRILWKGLRKLGRTAGN"
     gene            2229..>2993
                     /locus_tag="P5292_06625"
     CDS             2229..>2993
                     /locus_tag="P5292_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HRS38838.1"
                     /translation="MAQLIMPKMGESVAEATILKWLKKEGDRIEADEPVLEIATDKVD
                     SEVPSPVAGILKKRLFEEGQVVKVGEVIAEIGSEQDKVAATPASPAPAAQPAPAVTSN
                     GHKSAAPVAVSEPLVKSSSGSGRFYSPLVRNIAKQEGIGMQELESIPGTGMNNRVTKQ
                     DILQHIDNRQQGQQISAPAPVPAPAVATAAAPAYTAPAVSVSGAVEIIEMDRMRKIIA
                     DHMVMSKHTSPHVTSYVEADVTNLVKWRDKVKGAFEK"
BASE COUNT          704 a          794 c          794 g          701 t
ORIGIN      
        1 gcatgcaggc aataggcact ccggatgtga aatgtacgac cattgcccgg ggaatctcgg
       61 tcggcgatga gctggaatac gcagacgaag taacgctggg acgatccatc ctgcagcgag
      121 tcccttatga acaagccgta gcccgaccca acgaacgctg accccctact ccttccgtga
      181 agctttccgt cgtcattgtc aactacaacg tacagtactt tctggaacag tgcctgcgca
      241 gtgtcgagat cgcctgcaag cgggtatcca gtgaagtatt cgtggtcgac aacaactcgg
      301 tggacggatc ggtcgagatg gttcaggaga aattcccctc ggtgcggctg attgccaaca
      361 aggagaacct cggattttcc aaagcgaaca atcaggcgat ccggatcgct tccggcgagt
      421 acgtcctctt actcaatccg gacaccgtag tagaggaaga cacgtttgaa aagatcgtcc
      481 ggttcatgga cgaacatccc gacgccggtg gattgggtgt caagatgatt gacggtagcg
      541 gacgatacct gccggaatca aagcgcggct taccgactcc tgccgtggcc ttttacaaga
      601 tcttcggcct gagcgcactc tttcctcggt ccacccggtt cggcaggtat catctcgggt
      661 tcctcgataa aaatgaaacg catgtggttg atgtcctggc cggagcattc atgttgctac
      721 gacaagcggc cctgacgaaa tgcggtttgc tggacgagga ttttttcatg tacggggaag
      781 acatcgacct ctcctaccgg atccaaaaag ccggtttcaa gaattactat ttccccggca
      841 cacagatcat ccattacaag ggagaaagta cgaaaaagag cagcgtgaat tatgtcttcg
      901 tgttttacaa cgcgatgatc attttcgcga agaagcattt ttccccacag aacgccagcc
      961 tgttctcctt ttttatccgg attgcgatct acctacgtgc cggagcagcg gtcctgaccc
     1021 gggcattccg aaacctgttg ttgccggcac ttgatttcgg tttcctgttc gcgggattgt
     1081 actttctcca ggcgtattgg ggaacgaaca tgaacgtgcg ttatcctgag ttgtttcttc
     1141 aagcggctgt cccaacctat atcctctgct ggatcgtctg tatctatttc agcggcggct
     1201 acgatgtgcc catccgcatc gcgaaagttg ttcgaggagt tttcaccggc accctgatca
     1261 tcctggtggg ctatgccttg ctccccgagc attatcgttt ttcgagggca cttatcctgc
     1321 ttggagcggc ctgggctacg gcggctgcgg tagttattcg cctcgtgttg aatgtgcttt
     1381 ggaaaaagcg ctatgaattg gccaatgacc gacccaaacg tctgatcatt gtcggcagtg
     1441 aagaagaagg ttcccgggtc ttgtcactgc tcaaactgac cggcaccaat cacaatttca
     1501 tcggatttgt gcgaccggaa gaaataaccc ggtctcatcc tggaaagtcg caggaactga
     1561 acacctatcg gctcggtgat ctttctcagc tgaccagtat cgtcgaagtt tttgccatcg
     1621 acgaagtgat cttctgtgca cgtgaccttg cctccaaccg gatcattgcg ctcatggccg
     1681 gtatggcggg gaaagcaatt gaattcaaga tcgctccacc agacagcttg ttcatcatcg
     1741 gcagtaattc gattgacgat ccgggagaat tgtatgtgat cgatatcaac agcattggaa
     1801 agaaagagaa ccggcggaac aagcggtttc tcgatatcac cttctccatc gcgctgctgc
     1861 tggtcagtcc gatcctgatg tggttacaac agcgaccctg gacatttttc ctcaacttgt
     1921 tcaaagtgtt gctgggcagc ttgtcgctgg tcggctacaa ccttgcggct tccggattgg
     1981 atgaattgcc aaggatccgt cccggcatcc tgcatcccgg cgaccaactg cgcgatctta
     2041 cgacggattc ttccacctta caacgtctga ataccctgta cgctaaagat tatcagaccg
     2101 atacggatct gcgcatcctg tggaaagggt tgagaaaatt ggggcggacg gcaggaaatt
     2161 aacgcatcct tattgctaat tttgctaaaa ccccggtagc ggggtcatcc ctattctatt
     2221 cccaaccgat ggcgcaactc atcatgccca aaatgggcga aagcgtggct gaggctacga
     2281 ttctcaaatg gctgaaaaag gaaggcgatc gcattgaagc cgatgaaccg gtattggaga
     2341 tcgcgacgga taaagtcgac tccgaagtac cttctccggt tgccggtatc ctgaaaaagc
     2401 gcctctttga ggagggacag gttgtcaaag tcggtgaagt gatcgctgag atcgggtctg
     2461 aacaagacaa agtcgctgcg actcctgcca gtccggcccc ggcagcgcaa ccggctcctg
     2521 ctgtaacgtc taacggacac aaatccgccg ctccggtagc agtatccgag cctctggtca
     2581 agtcatcatc cggcagcggt cgcttttatt cgcctttagt gagaaatatc gccaagcagg
     2641 aaggcatcgg catgcaggaa ctggagagta ttccgggaac cggcatgaac aaccgggtta
     2701 ccaagcagga tattctccag catatcgaca accggcagca aggacaacag atcagcgctc
     2761 cggcacctgt ccctgcacca gccgtagcaa ccgcagccgc tccagcctat actgctccgg
     2821 cggtgagtgt ttccggcgcg gtcgagatca ttgaaatgga ccgcatgcgg aaaattatcg
     2881 cggaccatat ggtgatgtcg aagcatactt cgccgcatgt gacctcctat gtggaagcag
     2941 atgtgacgaa tctggtcaaa tggcgcgata aggtcaaggg tgcctttgag aag
//
LOCUS       DAOTON010000292         2985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25883_length_2985_cov_2.769625, whole genome shotgun sequence.
ACCESSION   DAOTON010000292 DAOTON010000000
VERSION     DAOTON010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25883_length_2985_cov_2.769625"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="P5292_06630"
     CDS             <1..337
                     /locus_tag="P5292_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38839.1"
                     /translation="VIVQKRIPKIFPAIIRRTGDYRFYYFCGDFADNPTKFRFAKLRG
                     ITGMKFLMYNAVDVTDRNRFFWEFYLPMMQRILREAWMESPRTKAGRTIFSWQSPSVS
                     SRPSSGPRS"
     gene            complement(276..1703)
                     /locus_tag="P5292_06635"
     CDS             complement(276..1703)
                     /locus_tag="P5292_06635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS38840.1"
                     /translation="MGYFFYYFTKFADHFILFYSGLLFATYFTLSALSAREIMGYIRR
                     NSFIDYRDILTAPSAPAVSIVAPAYNESKTIVENVRSLLNIHYVNFEVIIVNDGSKDD
                     TLQLMIDAYELEPVPYAFDETLHHKPIRAVYKSRIPSLSKLTVVDKVNGGKADALNAG
                     INVSRFDYFAAIDVDCILEYESLLKMVKPFMDATPDETVIAVGGVVRIANSCEVTEGK
                     ITEVNLPHQMIPRVQVLEYIRAFLLGRMAWSRLNGLILISGAFGMFDTRIVKSVGGYT
                     HRTVGEDMELVIRMRRHMEEKKRKYKVVYLPDPLCWTEAPSSFKVLGKQRNRWTRGSA
                     ETLWIHRKLIFNPRYNFLGMISMPFWFLFEWLAPIVETTAFLYLIMMAFLGILNVELF
                     ITLFLLIYAFAIMYSTAAILFEELSFHQYKKKRDIIRLLGTAFIEPIIIHPLTVYYAI
                     RGNLDLMTGVRNWGEMKRTGFARKK"
     gene            complement(1710..2858)
                     /locus_tag="P5292_06640"
     CDS             complement(1710..2858)
                     /locus_tag="P5292_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38841.1"
                     /translation="MNRLLQINWTDLQYSADEFSREVYTFPLHIRILIGITLTFVVVV
                     IVLLAVILGSRIHKTRRARTREELRRKYQPVFMELLFGSDEPFSEQDPKRLFDPLDLQ
                     TPFHQEILLEETIHLHENFSGETAARLELLFVKLGFHEVSKRRLREKRWDKVAKGMRE
                     LSLMNVREAEPLISPFLKSKNDILRKEARVALVNLSVKDHLSFLSRETEPLTDWDQAV
                     IYSMMSKMPELSIPDFSAWLDSKNPTVVEFCISMIGAFRQQESLPILIRLLNDPVEKR
                     KLLAIRALRELGAAAAEQPLLDLYPSANSEMRREILRTLEVIGSERSLQPIHQLLLQP
                     VSDLPLAIQAVRSLLATGPRGVQLVEQLSVHGDPKIQQVIRHARDKRL"
BASE COUNT          668 a          776 c          877 g          664 t
ORIGIN      
        1 ggtgattgtt caaaagcgaa tccccaagat ctttccggcg atcattcgcc ggacgggcga
       61 ctatcggttc tactacttct gtggcgattt tgccgacaac ccgaccaagt tccgcttcgc
      121 caagttgaga gggatcaccg ggatgaagtt cctcatgtac aatgcggtcg atgtgaccga
      181 tcggaaccga ttcttttggg aattctatct gcctatgatg cagcggatcc tgcgggaggc
      241 ctggatggaa tcaccccgca ccaaggccgg ccggactatt ttttcctggc aaagcccgtc
      301 cgtttcatct cgccccagtt ccggaccccg gtcatgaggt ccaggtttcc ccggatcgcg
      361 taataaacgg tcaggggatg gatgataatg ggttcgatga acgcggtacc caagagccgg
      421 atgatgtctc gcttcttctt atactggtga aacgacaatt cctcgaacag gattgccgcg
      481 gtcgagtaca tgatcgcgaa tgcgtagatg aggagaaaaa gggtgatgaa caactcgacg
      541 ttcaggatac ccaggaaggc catcatgatc aggtacagga aggccgtggt ttcaacgatc
      601 ggcgccagcc attcaaagag gaaccagaag ggcatactga tcatcccgag aaaattgtac
      661 cggggattga agatgagttt gcgatggatc caaagggtct ccgcactgcc ccgggtccag
      721 cggtttcgct gtttccccag cactttgaaa ctggagggcg cctcggtcca gcagagtggg
      781 tccggcagat agaccacttt gtacttccgt ttcttttcct ccatgtgacg gcgcatccgg
      841 atgaccaact ccatatcctc gcccaccgta cgatgcgtgt aaccgcctac ggatttgacg
      901 atccgggtat cgaacatccc gaacgccccg gagatcagga tgagaccgtt caaccggctc
      961 caggccatac gcccaaggag gaatgcccgg atgtactcga gcacctgcac gcgcgggatc
     1021 atctggtgag gcaggttgac ttccgtgatc ttgccctcgg tcacctcgca cgagttggcg
     1081 atgcgcacca cgccgcctac ggcgatgacc gtctcatcgg gtgtcgcgtc catgaagggt
     1141 ttgaccattt tcagcaatga ctcgtattca aggatgcagt caacgtcgat ggccgcgaag
     1201 tagtcgaatc gggagacgtt gatgcccgcg ttgagggcgt ctgccttgcc gccgtttacc
     1261 ttgtcgacga ccgtaagctt gctgagggaa gggatgcgcg atttgtaaac cgcgcgaatc
     1321 ggtttgtggt ggagggtttc atcgaaggcg tatggaaccg gctccagttc atacgcatcg
     1381 atcatcaact gcaaggtgtc gtccttactg ccgtcgttta caatgatcac ctcgaaattg
     1441 acatagtgga tgttcaatag cgagcggacg ttttcgacga tggttttcga ttcgttgtaa
     1501 gcgggagcga cgatcgaaac ggccggagcc gaaggcgcgg tcaggatatc ccggtagtcg
     1561 atgaagctgt tgcggcggat gtatcccatg atctcccggg cggaaagtgc cgaaagcgtg
     1621 aagtacgtgg caaataacaa accgctgtag aagaggatga agtgatccgc gaacttggtg
     1681 aagtaataga agaaatatcc catagcggat cagaggcgtt tatctctggc gtgacggatc
     1741 acctgttgga tcttgggatc tccgtgcacg gaaagttgtt cgacaagttg tacaccacgc
     1801 gggccggtgg ccaggaggga gcgtacggcc tggatggcca agggcaggtc gctgaccggc
     1861 tgtaaaagga gttggtggat cggctggaga ctacgctcgc ttccgatcac ttcgagggta
     1921 cgcaggatct cccggcgcat ctcgctgttg gcggaagggt agaggtcgag caggggttgc
     1981 tccgcagccg ctgctcccag ttcgcgtagc gcccggatcg ccagcagctt gcgtttctcc
     2041 accggatcgt tcaggagacg aatcaggatc ggcaacgatt cctgctgccg aaacgcgccg
     2101 atcatactga tgcagaactc gactacggtc gggttcttgg aatccagcca ggcggagaag
     2161 tcgggaatgg aaagctccgg cattttgctc atcatggaat agatcaccgc ctggtcccag
     2221 tcggtcagcg gctcggtctc ccgcgaaagg aacgacaagt gatccttgac cgacaggttg
     2281 acgagcgcca ccctggcttc tttccggaga atatcgttct tgcttttgag gaacggactg
     2341 atcaggggct ccgcttcacg gacattcatg agcgagagtt cgcgcatgcc tttggcgact
     2401 ttgtcccagc gcttctcgcg caaccgacgc ttcgatactt cgtggaagcc caacttcaca
     2461 aacagaagtt ctaagcgcgc ggcggtttcg ccggagaaat tctcatgcaa atggatggtc
     2521 tcttccagca ggatctcctg atggaaaggg gtctgaaggt cgagcggatc gaacaatcgt
     2581 ttcgggtcct gttcggaaaa cggttcgtca ctgccgaaca ggagttccat gaaaaccggc
     2641 tgatactttc tgcgtaactc ctcccgggtt ctggcgcgac gtgtcttgtg gatacgactt
     2701 cccagaataa cggccagcag cacgatgact actacgacaa acgtaagggt gatgccgatc
     2761 agtatccgga tgtgcagcgg gaaggtgtat acttcccggc tgaactcatc ggctgagtac
     2821 tgcaggtcgg tccagttgat ttgaagaaga cggttcaccg gtggaattgg gtacgggatt
     2881 atacccgtaa aaatccggaa agttcaccaa aaaaaagcgc cgatcgggcg cttcttctta
     2941 ggaatgggat gttaagggaa ttttcgttcc aactcctcga tttcc
//
LOCUS       DAOTON010000293         2983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25898_length_2983_cov_3.259904, whole genome shotgun sequence.
ACCESSION   DAOTON010000293 DAOTON010000000
VERSION     DAOTON010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25898_length_2983_cov_3.259904"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /gene="bshC"
                     /locus_tag="P5292_06645"
     CDS             complement(<1..990)
                     /gene="bshC"
                     /locus_tag="P5292_06645"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HRS38842.1"
                     /translation="MNGISSIALPLEETGLFSSIFIDFIEQRQWLDHFQLPKPDLQGL
                     LQVAQDAEWEKFDRKLLLNTLTAQYRELEESGTVISEAVKDNLEALSSPDSRTITTGQ
                     QLHPATGPLYVVFKILSTLNLCKQLNAVQQQHRFIPVFWLASEDHDLQEVRELQVAGK
                     TYTWLTEQSGPTGRISCKGLPEMLEQLKTDLGARPGIDKLLADVFNAYKSEYTLSLAT
                     RKLIHEWFGKAGLLMIDPDAAALKRSFIPVMQRDITRQEAPQAAKRLEEELSTHGSLP
                     LSIRSVNLFYLGEQFRLRIDTIPGGFRTPDERFNWTPEQLSAEIEAFPQRFSPN"
     gene            1225..>2983
                     /locus_tag="P5292_06650"
     CDS             1225..>2983
                     /locus_tag="P5292_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38843.1"
                     /translation="MTHTHAQISKRSVLAIAILFAVLSISIGRSTAQGVSTYTFSTAS
                     GFALDPLPGSFSMLIGPGQDDVSSSVTSIGFTFEFDGSYYSNFSANSNGLVSLGTTAV
                     STSGLNQLSNASINPKLAPYWDDLTTGTNGYVVTWLAGTAPNRRRVVEWQVTVPKGLT
                     NAAGAKFQLWILEGTRQVRFVYSSGISANLGQYSIGLSGSSGGFHAVTASNNTSSNSV
                     VKDNNTTAMNSGRTYLYTPPSLLSGCVLNATPANGSEGVSPITQLTWQANYGSPAAYN
                     VYFGTTTSPPLVSAAQAGTTYSLPQLLPNATYYWKVEPINALGTNTTCAVNSFQTNYL
                     LTYSPVWSDNIGFNSIASTGSSVASWRNGSNTDDNLSQAIPIGFNFTYQKSSFNSLLI
                     STNGFITLNTATTATGGGPGAYGYFNSNLTNGDPANNSPLIIAPLYEDLVCQGNPGTL
                     SSLSSSIHYMVNGTAPYRSLTIEWTGMEVYNNPGPNLNFQVTLFETIGEIEFTYGNME
                     AFNGTSTYSYSSSIGLNAIYVSNPPVVGEYFVQQQTNSESFSNTPANSLNVLPKCHSS
                     IHLYPVSGAIIPVVATVPDN"
BASE COUNT          697 a          772 c          707 g          807 t
ORIGIN      
        1 attcggactg aaccgttgcg gaaaggcttc gatttcggca ctcaactgtt cgggtgtcca
       61 gttgaaacgt tcgtcgggtg tacggaaacc gccggggatc gtgtcgatgc gcaagcgaaa
      121 ttgttcaccg aggtagaaca gattgaccga tcgaatcgat aaggggagcg aaccatgagt
      181 ggacaactcc tcttccagtc tcttcgccgc ctgtggtgct tcctgacggg taatatcgcg
      241 ttgcatgacc ggtatgaagg atcgtttcaa ggccgctgcg tccggatcaa tcatcaggag
      301 accagccttg ccgaaccact cgtggatcaa tttccgggtt gccagtgaaa gcgtatattc
      361 acttttgtac gcgttgaata catcagccaa caacttatcg attcctggtc gggccccaag
      421 atcggttttc aattgttcca gcatttccgg aagccccttg caggaaattc gtccggtggg
      481 cccggattgt tcggtcagcc aggtgtaggt ttttcccgcc acctgaagtt cccggacttc
      541 ctgcaagtca tggtcttcgg aagccaacca aaaaaccgga ataaaccgat gttgttgttg
      601 cactgcattc aactgcttgc atagattcaa cgttgaaagg atcttgaaga ctacataaag
      661 cggtcccgtt gccgggtgca actgctgccc ggtcgtgatc gtccgcgagt caggtgaaga
      721 aagcgcttcc agattgtctt ttacagcttc agagatgaca gttccgcttt cttccagttc
      781 acggtattgg gctgtaaggg tgttgagcag gagtttgcgg tcgaactttt cccattcagc
      841 atcctgtgca acctgcagca ggccctggag atccggtttt ggcaattgga aatgatcaag
      901 ccattgacgc tgctcgataa aatcaatgaa aatagaagaa aacagaccgg tttcttccag
      961 gggtagtgcg atacttgata tcccgttcat atttggtgaa ctcaaaggta aggattgcaa
     1021 gcatgaagga atttcggaca gcattctcgt ttaacgacca ttttttgaca tttaccgagt
     1081 aaagggaatc gattaccgat tcaggacagt atttcggtac tttttagcta tatttgatta
     1141 actaagctgt tgccactgga gtctggcaga ttctgacgga ctaccacttg gattttcccc
     1201 tcaccgaaaa gtgaggttcg tgacatgacg cacacacacg ctcaaatctc caagaggagt
     1261 gtattggcta ttgccatcct cttcgcagtg ctttcgattt ccattggccg ttcaacggct
     1321 cagggagtca gcacttatac cttctcgact gcttccggct ttgccctcga ccccctcccg
     1381 ggtagctttt ccatgctgat cggaccggga caagatgatg tatccagctc tgtaacgtcg
     1441 atcggattta cttttgagtt cgacggatcc tattattcca acttctcggc taatagcaat
     1501 ggacttgttt ccctcggaac tactgccgtg agtacttccg gattgaatca gcttagcaat
     1561 gcatcgatca atcccaaact ggcaccttat tgggacgatc tcactaccgg tacgaatggg
     1621 tatgtggtca cctggctggc cggtacggct cccaaccgtc ggcgtgttgt agaatggcag
     1681 gtgaccgttc ccaaaggact tactaacgct gccggtgcga agttccaatt gtggatcctc
     1741 gaaggtaccc gacaagtccg attcgtttac agttcgggga tcagcgccaa tttgggccag
     1801 tattcgatcg ggttgtccgg aagcagcggt ggatttcatg ccgtaacggc gtcgaataac
     1861 actagcagca attcggtggt aaaggataat aataccactg ccatgaacag tggccgtact
     1921 tacctgtaca cacctccctc cttgttatcc ggttgtgtcc ttaatgctac tcctgcgaat
     1981 ggaagtgaag gcgtcagtcc gattacgcag ttaacatggc aggcgaacta tggctctcct
     2041 gccgcttaca acgtttattt tggtactacc acttctcctc ccctggtttc cgctgctcag
     2101 gcaggcacga cctattcgct tccgcagctt ttgcccaatg caacctatta ctggaaagtt
     2161 gaaccgatca atgcgctcgg gaccaatacc acctgcgccg taaattcctt tcaaacgaat
     2221 tatcttctga cctactctcc tgtttggtcg gacaatatcg gtttcaactc catcgcttcc
     2281 accggcagct ccgttgccag ttggcgtaat ggctcgaata cggacgataa cctgagtcag
     2341 gcgatcccga tcggctttaa tttcacttac cagaagtcgt cctttaattc gttgctgatc
     2401 tcaaccaacg gattcattac gctgaatacg gctacgacag ctacgggtgg cggacctggc
     2461 gcgtatggct atttcaattc caatctcacc aatggtgacc ctgcaaataa ttcaccgttg
     2521 atcatcgcgc cattgtatga agacctggtt tgccagggca atcccggaac gctttccagc
     2581 ctaagcagtt ctattcatta catggtgaac ggaacggcac cctaccggtc attgacaatc
     2641 gaatggaccg gcatggaagt ctataataac ccgggaccta atctcaattt tcaagtaact
     2701 ctctttgaga caatcggcga gattgaattc acctatggta atatggaggc tttcaatgga
     2761 acctccacct actcttatag ttcctccatt ggattgaatg cgatttatgt ttccaatccg
     2821 ccagtggttg gcgagtactt tgtacaacag cagaccaatt ccgagagctt ttcaaatact
     2881 cctgctaatt cgctcaatgt cctgcccaag tgtcattcgt caatccatct ttacccggtt
     2941 tccggtgcta ttatccctgt cgtagcaact gtccctgata acg
//
LOCUS       DAOTON010000294         2983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25899_length_2983_cov_3.056011, whole genome shotgun sequence.
ACCESSION   DAOTON010000294 DAOTON010000000
VERSION     DAOTON010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25899_length_2983_cov_3.056011"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="P5292_06655"
     CDS             <1..1249
                     /locus_tag="P5292_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38844.1"
                     /translation="SLDKGCSFSLLGNGAASPPWRTALAVHPEDPELIFFSAGNKVNR
                     LVRSATTWRAVYAGGDLHDDVHELMFDPQGKYLLASTDGRLYATTDSGKTWSKASESV
                     SMAECWDVAIAPDTSLRVLSGLQDCGTVLYRSDTLGGAWFIVRGGDGMGVEFDAENDS
                     LAYHADGNNTIVGRSGNGGINWNTCTPKGSGRGYYLTALAAHPVYPDLVLSGFHDLFI
                     SRDEGRSWTRHRVDSALDPGLTMTAVAWAPSDSLTIYVAFENPVWSDQLKQRLYKSTD
                     GGDTWVDITSGLQSVAWMSITSLAVHPDHAEWITVGFRGGAKGKVMQNRSGGVAQRWQ
                     TVVEGLSGSNDVYALTYDWDAHRTLYAGTRRGVWHLQDNRWSDWSGGLPACMISGLAI
                     RKSDGLLVAGTHGWGVWMGHTHR"
     gene            1316..2341
                     /locus_tag="P5292_06660"
     CDS             1316..2341
                     /locus_tag="P5292_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HRS38845.1"
                     /translation="MSLLHSNFHYFRKLYIALGLVVFVLLLGVAGYMLLEDYNFLDAV
                     FMTVITVATVGYREVRELDTDGKIFTVFLIIFSIGAFAYAVSVITRYVIEGEFQLYFK
                     HYRVNKEIKQLTDHVIVCGYGRNGRQACEQLRSGNIPFVAIESNPAIIAQMQEEDNIL
                     FLEGDATKDEVLLDAGLDRAKALIAALPSDASNVFVVLTARDRNARLKIISRASEDGS
                     EHKLKRAGADNVIMPDKIGGTHMAALITKPDVLEFIDHITGRINIRLEEIMCRDLPER
                     MRNKTIRELEIRNKTGANIIGMKTRDGEYLINPDPATPMLPDTKLFVLGTQEQVMQFR
                     EIIRQSL"
     gene            2338..>2983
                     /locus_tag="P5292_06665"
     CDS             2338..>2983
                     /locus_tag="P5292_06665"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRS38846.1"
                     /translation="MSNSTTILITGSNGLLGQKIVYGLIGRDNIRCVASSRGENRLMR
                     KDGYEYVSLDITDPDQVRSVMERYRPDAVIHTAALTNVDACESRREEAVLMNVTATEY
                     LVHACNEHGSHLVHLSTDFVFDGANGPYIESDQPNPLSHYAATKLESERVVMATARSW
                     AILRTIIIYGVVDDNSRSNVVLWTLNSLKAGKSINAINDQFRSPTLARLTAASMQ"
BASE COUNT          642 a          805 c          870 g          666 t
ORIGIN      
        1 gtcgcttgac aaaggttgtt ccttctcctt gctgggcaat ggcgcagcct cgcctccctg
       61 gcggactgct ctggcagtac atccggagga tccggaactg attttcttta gtgccggtaa
      121 taaggtcaac cgacttgtcc gatcggcaac cacctggcgt gccgtctatg ccggcggaga
      181 tctgcacgat gatgtgcatg agctgatgtt cgatccgcag gggaaatatc tgctcgcttc
      241 gacggacggc cgcttgtacg ccacaaccga tagcgggaaa acatggtcca aagcttccga
      301 aagcgtttca atggcggaat gctgggatgt ggccattgct ccggacactt ccttacgtgt
      361 actttcgggt ctgcaggact gtggtaccgt tttgtaccgg tccgatacgc tcggtggcgc
      421 ctggttcatt gtgcgtggtg gtgacggtat gggagtagag ttcgatgcgg aaaatgattc
      481 gctagcctat catgccgatg gaaacaacac gatcgtaggg cgatccggga atggtggcat
      541 caactggaat acctgcacgc caaaaggttc cggccggggg tattacctca ccgcgcttgc
      601 cgctcatcct gtttatccgg atttggtcct gtccggtttt catgatctct ttatcagtcg
      661 cgatgagggg cgatcctgga cgcgacaccg ggtcgattcc gcgctggatc ccggtctgac
      721 catgaccgcc gttgcctggg cgccttcgga tagcctgacc atctatgtcg catttgaaaa
      781 tccggtatgg tcggaccaat tgaagcaacg cttgtacaag tcaaccgacg gcggtgacac
      841 gtgggtcgat attacctccg gattgcagtc ggtagcttgg atgtcgatta ccagcctggc
      901 tgttcatcct gaccatgctg aatggatcac cgtaggtttc cggggtgggg caaagggtaa
      961 ggtcatgcag aaccgaagcg gtggtgttgc tcagcgttgg caaacggtcg tggaaggatt
     1021 gtccggcagc aatgatgttt atgcgctgac gtacgattgg gatgcccacc gcaccctcta
     1081 tgccggcacc cgtcgcggcg tttggcatct gcaggataat cgctggtcgg attggtcggg
     1141 cggcttaccg gcttgtatga ttagtggcct ggcgatccgg aaatcggacg gcttgctggt
     1201 tgccggaaca cacggctggg gagtctggat gggtcataca catcgttgaa aaaatgaccg
     1261 gcgtcaccaa gggatgtccg gccccggaat taactttgac acgtatcccg tacgtgtgag
     1321 cctgctccat tccaacttcc attatttcag gaaactgtac atagccctcg gcctggtcgt
     1381 ttttgtgctg ctgttggggg ttgccggcta catgttgctg gaagactaca acttcctcga
     1441 cgccgtattc atgaccgtca tcaccgtggc gacggtggga taccgggagg tgcgggagct
     1501 ggatacggat gggaagatct tcacagtctt cctgatcatc ttcagtatcg gcgcttttgc
     1561 ctacgcagta tcggtgatca cccggtacgt gatcgaagga gaattccaat tgtacttcaa
     1621 acattatcgc gtgaacaagg aaatcaagca actcaccgat cacgtcatcg tgtgcggtta
     1681 cggccggaat ggccgccagg cctgcgaaca gctccggtcc gggaacatcc cgttcgtcgc
     1741 catcgagtct aatccggcga tcatcgcgca gatgcaggaa gaggacaata tcctgttcct
     1801 cgagggtgac gcgacgaaag acgaagtatt gctggatgct ggcctggatc gtgccaaggc
     1861 tctgatcgcc gccctgcctt cggacgcttc caatgtcttc gtggtgctga cagcacggga
     1921 ccggaatgcc cgactgaaga ttatcagtcg cgcttcggag gatggctctg aacacaagct
     1981 gaagcgtgcc ggagcggaca acgtgatcat gcccgacaaa atcggcggta cccacatggc
     2041 ggccctgatc accaagccgg acgtgttgga gttcatcgac cacatcacag gaaggatcaa
     2101 catccggctg gaggagatca tgtgccgcga tttgccggag cgcatgcgga acaagacgat
     2161 ccgtgaactg gagatacgaa acaagaccgg cgcgaacatc atcggaatga aaacacgcga
     2221 tggcgagtat ctcatcaacc ctgatcccgc tacccccatg ctccccgata ccaaattgtt
     2281 cgtactcggt acgcaggaac aagtgatgca gttccgggag atcatccgac aatccttatg
     2341 agtaattcca cgacgatact gatcaccggc agcaacggcc tgcttggaca aaagatcgtt
     2401 tacggcttga tcggcaggga caacattcgt tgtgttgcaa gttcccgcgg tgagaaccgc
     2461 ttaatgagga aagacggata cgaatatgtc tccctcgaca ttacggatcc tgaccaggtc
     2521 aggtcggtca tggagcgcta tcggccggat gcggtgatcc acaccgccgc gctcaccaac
     2581 gtcgatgcct gcgagagcag gagggaagaa gccgtgctga tgaatgtgac ggcgaccgag
     2641 tacctcgtgc atgcatgtaa tgagcacgga tcgcacctgg ttcacctttc gactgatttc
     2701 gttttcgacg gggcaaacgg tccctacatc gagtcggatc agccgaaccc cttgagtcat
     2761 tatgccgcta ccaaactgga atcggagcgg gtggtcatgg cgacggcgcg atcctgggcg
     2821 attctccgca cgatcatcat ttacggagtg gtcgacgaca acagccgctc caatgtcgtg
     2881 ctgtggaccc tgaactcact gaaggccggt aaatccatca atgcgatcaa tgaccagttc
     2941 cgctctccca ccttggcgcg cctgaccgct gcttcgatgc agg
//
LOCUS       DAOTON010000295         2976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_25971_length_2976_cov_3.944540, whole genome shotgun sequence.
ACCESSION   DAOTON010000295 DAOTON010000000
VERSION     DAOTON010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25971_length_2976_cov_3.944540"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="P5292_06670"
     CDS             <1..439
                     /locus_tag="P5292_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38847.1"
                     /translation="QSGNTVIADSVADTYQWVDCGLGYMILPGDTSAVLHPLNDGDYA
                     LIATLDGCTDTSACVVFRHSGIATTSSAYIRIFPNPSPGACTISAPEPISSLRITDSG
                     GRILRSLRPDHPESRVDGLMPGFYIVHGELQNGRRFREKLIVQ"
     gene            666..1613
                     /locus_tag="P5292_06675"
     CDS             666..1613
                     /locus_tag="P5292_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38848.1"
                     /translation="MEEPHKNSAQDPDAEKQPNEDSATPSDTAPEEPPVTIPPVNNPE
                     AIPQVPLENSMEVHHHTHSHGKKNWKSYIWEFLMLFLAVFCGFLAEYQLEHKIERDRA
                     KELARSFYTELKNDSVTAGIKVQNRLKQEAALMYMIRYFQDSNLTSVPKQFAINFEYG
                     ISFLSPSQFEPRTIMLDQLRNSGSLRYFKNEEFQRLTGELTVAIKNIYHRQELENDNR
                     QLYINPIVIRHYDYVFDAELKRDGKSIFEGLEEYERSDRFINFNLNNLDQVDRKGIVS
                     VLYFFKENVVSSTRKTHIQKYIEVNTALLKILRSEFDLD"
     gene            2109..2669
                     /locus_tag="P5292_06680"
     CDS             2109..2669
                     /locus_tag="P5292_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRS38849.1"
                     /translation="MKLHIKNMVCNRCVLVVRTELQRLGFDPVQVTLGEVDFDRELSA
                     EEKSLIAQHLETFGFTLIDDRKSRLIEQIKRLIIDLVHRQNSEMKVNLSDLLSDALHH
                     DYNYLSGLFSEVEGTTIERYYIAQKIERVKELLVYDELSLSEIAYLLNYSSAAHLSNQ
                     FKKVTGLSPSHFKKIGTDRRKPIDKV"
     gene            2752..>2976
                     /locus_tag="P5292_06685"
     CDS             2752..>2976
                     /locus_tag="P5292_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRS38850.1"
                     /translation="MTTQDKLVELPLEGVESEHCALIVDKGLAGVKGIRTHQVELNNR
                     RALIGADDPASVLEAIRAIRDLGYGVPSVQE"
BASE COUNT          740 a          794 c          776 g          666 t
ORIGIN      
        1 gcaatcgggg aacacggtca tcgcggactc ggttgccgac acctaccaat gggtagactg
       61 cggcctaggt tacatgatcc tacccggcga tacatcagcc gtattgcatc ccttgaacga
      121 cggcgactac gcgctgatcg cgacgctcga tggttgcacg gatacctccg cctgtgtggt
      181 cttccgccac agcggtatcg cgacaacttc atccgcgtat atccggatat ttccgaaccc
      241 gtctcccggc gcatgcacga taagcgcgcc ggagccgatc agcagcttgc gcatcaccga
      301 ttcgggcgga cgcatcctgc gttccctgcg ccccgaccat ccggaatcgc gcgttgacgg
      361 cctgatgccg gggttttata tcgtacacgg cgaattgcaa aacggacgtc ggttcaggga
      421 aaaactgatc gtgcaatgac tggtcggctt catcgccgtc tgttcggtac gacaacaggc
      481 gcagttgaag tccactgggc tggatacgcg cgggtcagga tcgatacgaa aacgcagaac
      541 gtccgggact tcttgaagtc ttgatcttct gctatattgg cgatcctggt tggtatcgta
      601 cgaatcgtat tcggagcatc tcaatgccgt acgatctgcg tattttccgc accggttggt
      661 aactcatgga agaacctcat aagaattcgg cccaggatcc ggacgctgaa aagcagccga
      721 acgaggattc cgcaacacca tccgacactg ctccggagga gccgccggtg acgattcccc
      781 ctgtaaacaa tccggaagcc attcctcagg taccgttaga gaattctatg gaagtacacc
      841 atcacacgca ttcccacggc aagaagaact ggaaatccta tatctgggaa tttctcatgc
      901 tgtttctggc agtcttctgt ggctttttgg ccgagtacca actggagcac aagatcgagc
      961 gcgaccgggc gaaggagctg gccaggagtt tttataccga actgaaaaac gactctgtga
     1021 ccgcggggat caaggtgcag aacaggttga aacaggaagc cgcgttaatg tatatgatcc
     1081 ggtattttca ggatagcaac ctcacctcgg ttccgaaaca gtttgccatt aactttgaat
     1141 acgggatcag ctttctgtcg ccatcgcagt tcgaaccaag gaccatcatg ctcgatcagc
     1201 tcaggaattc cggttcgctg cgctatttca agaacgagga gttccagcgt ctcaccggag
     1261 aattgaccgt ggcaatcaaa aacatctatc accggcagga actggagaac gacaaccgcc
     1321 agctatacat caatccaatc gtcatccggc actacgacta cgtgtttgac gccgaactga
     1381 agagggacgg caaaagcatt ttcgaaggac tcgaagaata tgaacggagt gatcggttta
     1441 tcaacttcaa cctgaacaac ctggatcagg tggaccggaa aggcattgtc agtgtactgt
     1501 actttttcaa ggaaaacgtc gtcagctcga cacgcaagac gcacattcag aaatacattg
     1561 aagtcaacac ggccctgctg aagatcctgc ggagtgagtt tgatttggat tgattcggac
     1621 atccaacagc aaagtgcgcc ggcggttgaa tgcgcaattc atgcatgagg gttgggaaaa
     1681 tgaattgtat catcaatgac cgggaattcc ggtttattcc caatgcgctt tcagtcatgt
     1741 ctgccaaagg ggcttatttt ctcctggtga agcagacctg tggtttgaat tttgaaaatc
     1801 ggtaacttcc cctcacttga caagcggtag tctaactctg ctgaacgact tcgcaagtaa
     1861 tcgcgaatac aaggtggttg cctacgacga ccagcccgcg ggcatgtcct ggtttctggg
     1921 tcggaaagac tctttatgaa cctccacaac cgtttttcgg agcgtcattt ttggtaactt
     1981 tccattttcc gaaaagcctc cagccgcggc aggcttgcct atcggcttcc tgtcatacgt
     2041 gtagcccggc gatcattgcc ggattccgcc cgacagttgc cgaactgact tttctggatc
     2101 attccaacat gaagctccat ataaagaaca tggtctgtaa ccgttgcgtg ctcgtggtga
     2161 gaaccgagtt gcagcggctt ggattcgatc cggtccaggt caccctgggc gaggtcgatt
     2221 tcgaccggga gctttccgcc gaagaaaaga gcctgatcgc gcagcacctg gaaacattcg
     2281 gattcacact catcgacgac cggaagagcc gcctcatcga gcagatcaag cgcctgatca
     2341 tcgacctggt ccaccgtcag aacagtgaaa tgaaggtcaa tctttccgat ctgctgagtg
     2401 acgccctgca ccacgattac aattatctct ccggtctctt ttcggaggta gaaggcacaa
     2461 ccatcgaacg ctattacatc gcccagaaaa tcgaacgggt caaggagttg ctggtgtacg
     2521 acgaattgtc gctttccgag atcgcctatc tgctgaatta ttccagcgcg gcacacctga
     2581 gcaaccagtt taagaaggtg acgggtctat cgcctagtca tttcaagaag atcggtaccg
     2641 accggcgtaa gccgatcgac aaagtgtaaa ttctgcaact taatccgata aaggtgtaag
     2701 gccagtcggg gaacgcgaca cgagatttgc attgtaatac atgcaaactc catgacaaca
     2761 caagacaagt tggtcgaact cccgctcgaa ggagtcgaaa gtgagcactg cgccctgatc
     2821 gtcgacaaag ggctggcagg tgtgaaaggc atccggacgc atcaggtcga actaaataac
     2881 aggcgtgccc tcatcggagc cgacgatccg gcgtccgtcc tggaagcgat ccgcgcgatc
     2941 cgggaccttg gttacggagt cccttccgtg caggaa
//
LOCUS       DAOTON010000296         2968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26051_length_2968_cov_3.737384, whole genome shotgun sequence.
ACCESSION   DAOTON010000296 DAOTON010000000
VERSION     DAOTON010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26051_length_2968_cov_3.737384"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="P5292_06690"
     CDS             <1..145
                     /locus_tag="P5292_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HRS38851.1"
                     /translation="TSFGSYSGAVPNEKITWGKLGIDTPKFIVESDATIVAPLIFAWV
                     LGW"
     gene            212..1069
                     /locus_tag="P5292_06695"
     CDS             212..1069
                     /locus_tag="P5292_06695"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRS38852.1"
                     /translation="MFRLLNQPYPREENPLKRLRFVSISAVIVFLFLWVFRPFGIDRV
                     EVESLDLFIAGYGLVTFLVAGLLVVVVPILIPAWFQESGWTIGKNILFYTILITGIGL
                     GNTFYTAKVLGKHVGPEGFLFFQISTLSVGAVVASMFTLLRYTRNLRFHEAEAKSLDQ
                     ELHEQAVPATGETIVLTAENGKESLSLKLDDLLFIESADNYSSVCYREGKLLRRQLLR
                     GSLRMLEQQLSGSSIVRCHRSYLVNLRSVRRITGNSQGYRAHFENLEDGIPVSRKAGP
                     ELHRYFRRS"
     gene            1224..1901
                     /locus_tag="P5292_06700"
     CDS             1224..1901
                     /locus_tag="P5292_06700"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HRS38853.1"
                     /translation="MKIMLVIHVITGFTGFFVAPVALAVKKGGNAHRRWGKVFFWSMT
                     GAAGSALVMAPYKQNWFLTLVAVFSFYMAFSGYRAVYRKKVRSRSEIPLIDWVISGLN
                     ALFAVGLLILGITLLPDAFGYISIVFGLIGLSYTRQDLHFFQRPVENKKQWLVNHITG
                     MMGGYIAAVSAFSAVNLGFSWMPTVLQWLWPTMIGVPVIFYFTSQYRRHRKSGRSDHE
                     HYEIRNS"
     gene            2007..>2968
                     /locus_tag="P5292_06705"
     CDS             2007..>2968
                     /locus_tag="P5292_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRS38854.1"
                     /translation="MDRFSFLSNLDVNQAEELYRQFLQQPDSLDAGWRNFFSGFEFAR
                     TNYDLDAGGSVVPEHLRKEFNVINLINGYRTRGHLFTDTNPVRERRKYTPTLDLVNFG
                     LGEEDLDQEFQAGSLIGIGKARLRDIVQHLHQTYCASIGAEYKYIRNPEIVEWLEQRM
                     EGRKNTPAFSIERKRRILEKLNEAVAFENFIHTKFVGQKRFSLEGCETLIPALDSVIS
                     SGADLGIREFVIGMAHRGRLNVLANILGKTYEEIFTEFEGLEYDEEGYAGDVKYHLGY
                     SSDVTTPSGKSVHLSVAPNPSHLEAVDPVVQGIVRSKIDNTPGG"
BASE COUNT          645 a          776 c          801 g          746 t
ORIGIN      
        1 gaccagcttt ggttcctatt caggcgctgt cccgaacgag aagatcacct ggggtaaact
       61 ggggatcgat actcccaagt tcatcgtgga aagtgatgcg accatcgttg ctcccttgat
      121 ttttgcctgg gtgctcggtt ggtgatcagc gtgtaattaa cttcatgcgg cggaagtagc
      181 gattgctgct tccgctttct attttcgttg gatgttccgg ttgttgaacc agccctaccc
      241 gcgcgaagag aacccgctga agcgactgcg attcgtctcg atcagtgccg tcatcgtttt
      301 cctattcctc tgggtcttcc ggccttttgg tatcgaccgg gtggaagtgg agtcgctcga
      361 tctgttcata gccggttacg gacttgttac cttcctggta gcaggactac tggtcgtggt
      421 ggttcccatc ttgattccgg cctggtttca ggaatccgga tggaccattg gcaagaacat
      481 cctgttctat accatcctga tcaccggcat cggacttggg aatacctttt acaccgcaaa
      541 ggttcttggc aaacacgtcg gaccggaagg attcctcttc ttccagatca gcaccttatc
      601 ggtcggagcg gtcgtggctt ccatgttcac gctcctccgg tatacccgga atctccgctt
      661 ccatgaggcg gaggcgaagt ccttggatca ggaactgcat gaacaagccg tacctgcgac
      721 tggcgaaaca atcgtgttaa cggcggaaaa tgggaaagag agcctgagcc tgaagctgga
      781 cgatctgttg ttcatcgaat ccgcagacaa ttattcctcg gtttgttatc gggaagggaa
      841 actgctgcgg cgccagttgc tgcggggaag tcttcggatg ttggaacagc agttaagcgg
      901 ttcttccatc gtgcggtgtc atcgttctta tctcgtcaat ctccgatcag tccgacgcat
      961 cacgggcaac tcgcagggtt atcgggcaca ttttgaaaac ctggaagatg gcataccagt
     1021 gtccagaaag gcaggtcctg aattgcaccg gtactttcgg aggtcttgat ccggttgcca
     1081 aacatcccag tcgggtgtac aaacggtctc cagccttgcc aatcgtccct aaacttgcat
     1141 tttggcccaa attttaatct tttcacttag tctgggtgta tccttgtgcc cgaatcaaac
     1201 catcggaaac aatggaaaca ctcatgaaaa ttatgctggt tatccatgtg ataaccggct
     1261 tcaccgggtt ctttgttgcc ccggtcgcgc tcgcagtcaa aaagggcggc aacgcccacc
     1321 gtcggtgggg taaagtgttt ttttggagta tgaccggagc agcgggttct gcgctcgtca
     1381 tggcaccata taagcagaat tggtttctga cactggtggc tgtattcagc ttctatatgg
     1441 ctttttcggg ttaccgggca gtttatagaa agaaagtgcg cagccggtcc gagataccgt
     1501 tgatcgactg ggtcatttcc ggtctgaatg cactctttgc agtaggccta ttgatcctgg
     1561 gcatcaccct cctgcccgat gcgttcggtt acatatccat tgtctttgga ttaataggcc
     1621 tttcttacac ccggcaggat cttcatttct tccagcggcc ggtcgagaac aaaaagcagt
     1681 ggctggtgaa tcatataacc ggtatgatgg gtggttacat agccgcggta tccgcatttt
     1741 cagccgttaa tctgggattc tcctggatgc caaccgttct tcaatggttg tggccgacga
     1801 tgataggtgt tccggtcata ttctacttca ccagccaata tcgccgtcat cgcaaaagcg
     1861 ggcgatcgga ccatgaacac tatgaaatcc gcaattcatg agcggtaacc cgcagggccg
     1921 ggacacgtaa agggcgcagt ccggaaccag cgcgcaggga acttcgtacc ttcgcgtttg
     1981 tttagtgtac cggaataacc cctcccatgg atcgattctc cttcctctcc aacctggatg
     2041 tcaaccaggc ggaggaactt taccggcaat ttctccagca gcccgatagc ctcgatgccg
     2101 gctggcgtaa ttttttctcc gggtttgagt tcgcacgtac caactatgat ctggatgccg
     2161 gcggaagcgt ggtgccggag cacctgcgaa aggaattcaa cgtcattaac ctgatcaatg
     2221 gttaccggac ccggggccac ttgtttacgg atacgaaccc ggttcgggaa cgtcggaaat
     2281 ataccccgac ccttgacctg gtcaattttg gacttggcga ggaggatctt gatcaggagt
     2341 tccaggccgg ttcactcatc ggtatcggga aggcccggct gcgcgatatc gtgcagcacc
     2401 tgcaccagac ttactgtgct tccatcggtg ccgaatacaa atacatccgg aatccggaga
     2461 tcgtcgagtg gctggagcag cggatggaag gccggaagaa tacccccgcc ttcagcatcg
     2521 agcggaagcg gcgaatcctg gaaaaactca acgaggcagt tgcctttgag aatttcattc
     2581 ataccaagtt cgtcggacaa aagcgctttt cactcgaagg atgtgaaacg ctcatcccgg
     2641 cgctcgactc ggtcatcagt tcgggtgccg atcttggcat ccgggaattt gtgatcggga
     2701 tggcccaccg cgggcgcctc aatgtgttgg cgaacatcct gggcaaaacg tacgaagaga
     2761 tcttcaccga attcgaagga ctcgagtacg acgaagaagg ctatgcgggt gatgtgaagt
     2821 atcacctcgg ctattcttcc gatgtgacga cgccgtccgg caagtctgta cacttaagtg
     2881 tcgcaccgaa tccttcccat ctcgaagcgg ttgatccggt tgtccagggt atcgtgcgtt
     2941 ccaagatcga caataccccg ggcggaca
//
LOCUS       DAOTON010000297         2961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26120_length_2961_cov_5.443909, whole genome shotgun sequence.
ACCESSION   DAOTON010000297 DAOTON010000000
VERSION     DAOTON010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26120_length_2961_cov_5.443909"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..996)
                     /locus_tag="P5292_06710"
     CDS             complement(55..996)
                     /locus_tag="P5292_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38855.1"
                     /translation="MRLIFFSLLACYFATANAQNLIVNGDLEIMNCPVGLSCLDSASG
                     WSNPAIFPGPGGSPDLFNACAGSSNFVNVPDNFAGFQLARSGVGYGGVFVYYPDFPNY
                     REFAQVELTGPLSAGDCYEVEFFVSPGDLMAFAAADIGLALSTTRITGVTNNLPLPLT
                     PVLTNTAGVINDTSGWTRVHGTITAGGGEAFAIIGNFEYDAQIDTSVLDPNSTMNGAY
                     FFIEDVSIRPCPVGLNELSDNFPRIGPNPFEDVVCFRQVQQPVYWELFDAFGRIVERA
                     TIEKDQKLDLSGLPAGSYWYRCTEERGAFTTGKLIRQ"
     assembly_gap    1325..1424
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1425..2432
                     /locus_tag="P5292_06715"
     CDS             <1425..2432
                     /locus_tag="P5292_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38856.1"
                     /translation="RLIRASNGDLIMTGYAVDASMTLPATIFARRLDPAGNVRWTQSW
                     GSGYGMDANAITETLDGGFVIAGNGIFDTTGIPVQYILKCSGSGDSLWCRTWGQSEYS
                     NATAIVQSSDGSLFVGSNGYYTGLSYQIETTKLDSSGQVIWNKVMGDQRENIVKDIAL
                     VNDSEVAIIGTYADPDSAYQVFFARLSSNGDSVWVATHGGLGSEDIHHGILSEDGGYV
                     AVGGTSSFGSSYMAYIIKTDPNGVLTGEQSFISPPGAFLIYPNPASESFRILLPPTQI
                     NTACKLKIMNSLGVTVHYVTLPPGSCPKVDCSDLPPGWYTVVVEGKDAYIASQRLVVV
                     R"
     gene            complement(2655..>2961)
                     /locus_tag="P5292_06720"
     CDS             complement(2655..>2961)
                     /locus_tag="P5292_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRS38857.1"
                     /translation="TYQRCKEQLELGVNEMKNFVRMIYQPFTAEQISKKIAEIVRPEG
                     CQAELEIIFQKIEDLHSACPNHRGDWYFTGNYPTPGGNRVVSKAFINYVEGVNARAY"
BASE COUNT          700 a          765 c          747 g          649 t
ORIGIN      
        1 tctggaaaaa tcagttcatc caatttattc gaaagacctt tgctgaacgc gaccttattg
       61 ccggatcagt tttccggttg tgaaagcgcc gcgctcttcc gtacaacggt accaatagct
      121 cccggccggg agtccgctta aatcgagctt ctggtctttc tcgatcgtcg ctcgctcgac
      181 gatacggccg aatgcatcga acaactccca ataaaccggt tgctgcacct gacggaagca
      241 cacgacatct tcgaacgggt tggggccgat ccgcgggaaa ttatcactca gctcattcaa
      301 accgaccggg caggggcgga tgctgacatc ctcgataaaa aagtaagcgc cgttcatggt
      361 ggagttcggg tcgaggaccg aggtgtcgat ctgcgcatcg tactcgaagt tcccgatgat
      421 cgcgaaagct tctcctccgc cggcggtgat ggttccatga acgcgtgtcc agcccgaggt
      481 gtcgttgatg acaccggcag tattcgtgag cactggcgtg aggggcaagg gaagattatt
      541 cgtgacgccg gtaatccgcg tcgtagaaag cgcgaggccg atgtcggccg cagcgaacgc
      601 catcaggtcg cccgggctga cgaagaactc gacttcgtag cagtcgccgg ctgacagcgg
      661 gccggtcagt tccacctggg caaactcccg gtagttgggg aagtccgggt agtagacgaa
      721 gacgccacca tagccaacac cacttcgcgc aagctggaag cctgcaaaat tgtccggaac
      781 gttaacgaaa ttcgaggaac cggcacacgc gttgaaaaga tcgggtgagc cgcccggtcc
      841 gggaaagatg gccggattac tccagccact ggccgaatcc agacaggata atccaaccgg
      901 acaattcatg atttccaggt cgccgttgac gattaggttc tgggcattag ccgtagcgaa
      961 atagcaagca agcaaggaaa agaaaataag cctcatctta acagttgtag cggtagacca
     1021 tacaggacta ctattttcgt aatcccatct aaagtacatg tttcttcttt tatcttattc
     1081 gataacgcga ttgatgattc taagcaggag tctcccaaca ttaatttcaa gaaaaacaga
     1141 taaaatataa atatttacgt ttgtaattcg taacaattgt tctcgtgcca tcatgattct
     1201 atcatttacg aaaaccttgg acgctgagcc tatttcccct accatcacct acaactacat
     1261 cctcaacgca caggataacg aatacgccgt aaatgtaagt ggcatgccac agggcatcta
     1321 caaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggtta attcgtgcat
     1441 ccaacggcga tctgatcatg actggttatg cggtggacgc ttccatgacc ctgccggcta
     1501 ccatcttcgc cagaaggctg gatcctgcgg gaaatgtacg ttggacccaa agctggggtt
     1561 cgggttatgg catggatgcc aatgccataa ccgagacact ggacggagga ttcgtcattg
     1621 ccggtaacgg tatctttgac acaaccggaa ttcccgtgca gtacatcctg aagtgcagcg
     1681 gctcgggtga tagtctttgg tgcaggacct ggggacaaag cgagtactcc aatgccacgg
     1741 cgatcgtgca gtcctccgac ggctccttgt ttgtggggtc aaacggttat tataccggtc
     1801 tgagttatca gatcgaaaca accaaactcg actcgagcgg tcaggtcatc tggaataagg
     1861 taatgggcga tcagcgggag aatatcgtca aggacatcgc cctggtcaac gattcggaag
     1921 tggcgatcat cggaacctac gcggatccgg acagcgccta ccaggtgttc tttgcaagat
     1981 tgagttcgaa cggagattcc gtatgggtag caacccatgg cggattgggc agtgaggata
     2041 tccaccacgg catcctctcg gaagatggtg gatacgtcgc cgtgggtggc acttcgagct
     2101 ttggttccag ctatatggcg tacatcatta aaactgaccc aaacggagtg ttgaccggag
     2161 agcagagctt catcagtcct cctggagcat tcctgatcta ccccaaccct gcttccgaat
     2221 cgttccgcat cctgcttccg ccgactcaaa tcaatacggc gtgtaagctg aagatcatga
     2281 atagcctggg ggtaacggtc cactacgtga ccttaccccc gggttcatgc ccaaaggtcg
     2341 actgctccga tctgccacca ggctggtata ccgtcgtggt cgaaggaaag gatgcctata
     2401 ttgcttcaca gcgactggtg gtcgtgaggt agaaatcttc cgataagttg gactggctgg
     2461 ggaagttttt tgccaccgcc acccaccttc gtcaaccgaa caagcagtcg aaaaaagttt
     2521 atacggttgc tggtgttgca ttacaccgta gcatgcccct accacacaag cgagttccta
     2581 aaaacggtca accccagtta ggcaatgaca actcggaacc cgaaacccgg aacccggaac
     2641 ttccaaaccc ttgttcagta cgcccttgca ttaaccccct ccacataatt gatgaacgcc
     2701 ttgctcacca cgcggttgcc acccggtgtc gggtagttac cggtgaaata ccagtcgccg
     2761 cgatggttcg gacaggcgct gtgcaggtct tcgatctttt ggaaaatgat ctccagctcc
     2821 gcctggcaac cttcgggcct gacgatctcg gcgatctttt tactgatctg ctcggcggtg
     2881 aaaggctgat agatcatgcg cacgaagttc ttcatctcgt tcacgcctaa ttccagttgc
     2941 tccttgcagc gctggtaggt c
//
LOCUS       DAOTON010000298         2961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26124_length_2961_cov_3.584652, whole genome shotgun sequence.
ACCESSION   DAOTON010000298 DAOTON010000000
VERSION     DAOTON010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26124_length_2961_cov_3.584652"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..699
                     /locus_tag="P5292_06725"
     CDS             160..699
                     /locus_tag="P5292_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38858.1"
                     /translation="MRILFGFTLACVVSSCAWTDHSPENGWYEENFDNLNHWKKDAVI
                     SSNQWRSGMYATYADSAHPVSESFTMHFNEVRFHHYTGVKATAWVFNPSNDTSGQVVL
                     EVVDMRSGEPERVLSEQVILGTDFREFNSWGKVQLTLDFPTLPGPHQVLRFYLASLNC
                     RKSYMDDVTLEFQRELIHR"
     gene            complement(705..1925)
                     /locus_tag="P5292_06730"
     CDS             complement(705..1925)
                     /locus_tag="P5292_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38859.1"
                     /translation="MYKLHPSTIALGLFLYAIYFVLVPDCGHVYDNQCWSDWAAYYLE
                     KGFRHSYESGTNYFPGHLYQLKLFSLFFDSKEDLAAHIYQLKRITLLGDLVGAFLLAS
                     CARVRWQQHALLLVLLLNPAFLHNTLVWGQFDTVFSALVFGSFIFLHKRRYLLSAISY
                     LLALNFKFQAILFLPAMVLLFVYASGLKPDLRKMLRGLAILVAMQLVILAPFIVSKSM
                     GRLWEVVDSLGGRYSYLSLYADNFWYLSMSGNLRLTGDFNTWMGLNYKTWGLIMTAAT
                     LLLVFFPLLLVLYKRWKGQMVTYDLEDAAGLLGLSVLAFFFFSTQMHERYTFPAFYFI
                     AFLALQSRWWWLFGVFCMAYLLNNERALPHFLHESPTILSSPPAVAVLYLIVLVSVLV
                     RLWMPKKSAVRTGF"
     gene            complement(2027..>2961)
                     /locus_tag="P5292_06735"
     CDS             complement(2027..>2961)
                     /locus_tag="P5292_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRS38860.1"
                     /translation="EQILIREGILRVNPNQINEDNFNSDSYLAGQYEGLVKSDLMDLN
                     PSSGSNGTRKVRMEDGILLPRYRLPTEAEWEYAALSQIGNTVYERVTDRRQYPWNGHI
                     VRNKDEKYKGQMMANYKRGRGDQMGTAGFLNDNADRPAPVQSYWPNDFGLYCMAGNVN
                     EWVMDVYRPLSPEDKADFNPYRGNVFKTQIRDEEGTIVEKDSLGRVPMRDVTEEENVD
                     RRNYTKADNINYLDADEAEYIKYDYGATSMVNDKARVYKGGSWKDRAYWMSPGTRRFL
                     DENQSTDDIGFRCAMVRVGSPVGLGAKPKTKLKR"
BASE COUNT          718 a          808 c          781 g          654 t
ORIGIN      
        1 acccgcgatc tatatcctgc tggccgcgct ggtggccctg gacatgctga tctatcagac
       61 ctccgccagc ctctatggcc tggtcatcat cttgctgggc attcctgttt atgcctttct
      121 ccaaaacaaa cagcagaaaa acggccgcct ttcctgacca tgcgcatcct gttcggtttc
      181 accctcgcct gcgttgtttc ttcttgtgcc tggaccgatc actcaccgga gaacggttgg
      241 tatgaggaga atttcgacaa cctcaatcat tggaaaaaag atgccgtgat ttcatccaat
      301 caatggcgta gcggaatgta tgcgacgtac gcggacagcg ctcacccggt cagtgagtca
      361 ttcaccatgc acttcaacga agttcgcttt catcattaca cgggcgtaaa ggctactgcc
      421 tgggtcttca atccttcgaa cgataccagc ggacaggtcg ttctcgaggt ggtggatatg
      481 cggagcggcg aacccgaacg cgtgctttcc gaacaagtca tccttggaac agacttccgg
      541 gagttcaaca gttggggtaa agtacaattg acgctcgact tccctaccct accggggccc
      601 caccaggtgc tgcgttttta tcttgcgtcc ctcaattgca ggaaatcata catggacgat
      661 gtcaccctgg aattccaacg cgaactcatc catcgctgaa ctcgtcaaaa cccggtacgc
      721 accgctgatt ttttcggcat ccacaagcgc acgagtacgc tgaccagtac gatcaggtac
      781 agcactgcca cggcaggggg actcgagagg atcgtaggtg attcgtgtag aaagtgtggc
      841 aatgcacgtt cgttgttcag caggtaggcc atgcaaaaga ctccgaacag ccaccaccac
      901 cgggactgaa gcgcgaggaa ggcaatgaag tagaatgctg gaaacgtata gcgctcgtgc
      961 atctgcgtgc tgaagaagaa aaatgcaaga accgacaagc cgagcaaacc ggctgcatct
     1021 tccaggtcat acgtcaccat ctggcctttc cagcgcttat agagcaccaa tagcagtggg
     1081 aagaaaacca gcagcaaggt cgcagccgtc atgatcagcc cccaggtctt gtaattcaat
     1141 cccatccagg tattgaagtc accggtcagc cgcaggttcc cgctcatgct gaggtaccag
     1201 aagttatcgg catacagcga aagataggag tagcggccac ccagggaatc gaccacttcc
     1261 catagtcgcc ccatactttt cgacacgatg aacggagcca ggatcaccag ttgcatcgcc
     1321 accaggatgg ccaggccgcg cagcattttt ctcaaatccg gttttaatcc ggacgcgtag
     1381 acaaaaagca acaccatggc cggcaggaag aggatcgcct ggaacttgaa gttcagcgcc
     1441 aacaggtagc tgatggccga aagcagatat cgccttttat gcaggaaaat gaatgatccg
     1501 aagacaaggg cggaaaagac cgtatcgaat tgcccccaca ccagggtgtt gtgcaggaag
     1561 gccggattca gtaagagcac caacaacaaa gcatgttgct gccagcgcac gcgcgcacac
     1621 gacgccagca ggaaggcacc aacaaggtca cccaacaagg tgattctctt gagctgataa
     1681 atgtgagcag ccaggtcctc tttgctgtca aagaacagcg agaagagttt taactgataa
     1741 aggtgtcccg gaaagtaatt ggtacccgac tcataactgt gccgaaaccc tttttcaagg
     1801 tagtaagcgg cccaatctga ccagcactga ttatcgtaga cgtggccgca gtcgggaaca
     1861 agaacaaaat agatagcata taagaaaaga cccagcgcta tggtcgaagg atgaagcttg
     1921 tacatcgtgg gagcgcggaa gctctgggcg gccaatactt ggcgatttac ccggcgagca
     1981 aaaataaaga accccgcgga ttggcggggt tctttgcttc gatttcttat cgcttcagtt
     2041 tcgttttcgg cttggcgcct aatccgaccg ggctgcctac gcgcaccatg gcgcagcgga
     2101 agccgatgtc gtcggtggac tgattctcgt cgaggaatcg gcgggtaccg ggcgacatcc
     2161 agtaagcgcg atctttccag gagccgcctt tgtaaacgcg ggccttgtca ttcaccatac
     2221 tggtagcgcc gtagtcgtac ttgatatact ccgcctcatc cgcatcgagg tagttgatgt
     2281 tgtcggcttt ggtatagttg cgacggtcaa cgttttcttc ttccgtaaca tcgcgcatgg
     2341 gaacacggcc caggctgtcc ttctccacaa tcgttccttc ctcatcgcgg atctgtgtct
     2401 tgaacacgtt accacgatac gggttgaagt cggccttgtc ttccggcgaa agcggacgat
     2461 aaacgtccat cacccactca ttcacgttac cggccatgca gtacaggcca aagtcgttgg
     2521 gccagtagga ttgtacagga gccggacggt ccgcgttatc attcaggaag ccggcggtac
     2581 ccatctggtc accgcgaccg cgcttgtagt tggccatcat ctggcctttg tacttctcgt
     2641 ccttattacg cacgatgtgt ccgttccagg gatactggcg acgatccgtt acacgctcat
     2701 ataccgtgtt gccgatttgc gacaaagcgg cgtattccca ctccgcctcg gtggggaggc
     2761 ggtagcgggg aagcagtata ccgtcttcca tgcggacctt acgggtacca ttggaaccgg
     2821 agctgggatt gaggtccatc agatcggact tcaccaaacc ttcgtattga ccggccagat
     2881 aagagtcaga gttgaagttg tcttcgttga tctggttcgg gttgacacgc aaaatacctt
     2941 cgcggatcag gatctgttcg t
//
LOCUS       DAOTON010000299         2960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26139_length_2960_cov_2.628916, whole genome shotgun sequence.
ACCESSION   DAOTON010000299 DAOTON010000000
VERSION     DAOTON010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26139_length_2960_cov_2.628916"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="P5292_06740"
     CDS             complement(<1..911)
                     /locus_tag="P5292_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRS38861.1"
                     /translation="MENNNPTIEKYTSKELVTDQEVRWCPGCGDYSILKQAQSVIPDL
                     GIPRENIVFVSGIGCSSRFPYYMETYGMHSIHGRATAIASGLKAARPELSIWIVTGDG
                     DGLSIGGNHTIHLLRRNFNVNVLLFNNEIYGLTKGQYSPTSEEGKITKSTPMGSVDHP
                     FNPIALCMGADATFVARSMDRDPKHLQSVLRQANEHKGASFVEIYQNCNVFNDGAFEV
                     FTEKASKKKETLFMESGKPLVFGENNELGIKLDGFTPVVVNLAEGNSVNDCWIHDERD
                     RVKAGILSRFFDNPRTMSEKHLPRPFGV"
     gene            complement(945..2786)
                     /locus_tag="P5292_06745"
     CDS             complement(945..2786)
                     /locus_tag="P5292_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRS38862.1"
                     /translation="MERQNEVLENVIIKFAGDSGDGMQLTGTQFTNTAALFGNDLSTF
                     PDFPAEIRAPQGTLAGVSGFQLHFGSVEIFTPGDYCDVLVAMNAAALKANLRFLRKSG
                     TIIVNTDGFDPKNLRLAGYDDKNPLEDGTVDSFRVIKLDVTKMTRTALEGSGLGVKEI
                     DRAKIMFVLGFLYWMYNRNMEHTLKFIEDKFSKKPEIRDANVKVLKAGYNFGETREEF
                     TTRYTINPAKIASGTYRNIMGNQAVAIGLIAAAEKSGLPLFLGSYPITPASDILHELS
                     RHKNYNVKTFQAEDEIAAIASAIGASFGGALSVTTSSGPGIALKTEAMGLALMLELPL
                     VIVNVQRGGPSTGLPTKTEQSDLLQAVYGRNGESPIPVIAAATPSDCFDMAYEACRIA
                     VQHMTPVILLTDGYIANGAEPWKFPKAADLPAIPVKFATEKNNGDKFLPYKRDERLAR
                     PWAIPGTKGLEHRIGGLEKQHETGNISYDAANHEFMVRMREAKVEKIADYIPEIKPEI
                     GNAKGKLLMLGWGSTYGAIKSAVLQAREKGLDVSYAHLRHIRPFPKNLGELLYAFDQV
                     IIPEMNTGQLIKMIRDKYLVPAVGFNKIQGMPFTAEEILKKIEETLK"
BASE COUNT          612 a          847 c          808 g          693 t
ORIGIN      
        1 acgccgaacg gacgcggcaa gtgcttttcg gacatcgttc ttgggttgtc aaagaagcgg
       61 gaaagaatac cggctttcac ccgatcccgc tcgtcgtgga tccagcaatc gttgaccgaa
      121 tttccttccg ccaggttcac gacgaccggc gtaaatccat ccaacttgat acccaattcg
      181 ttgttctccc cgaagaccaa tggcttgcca ctctccatga agagcgtctc cttcttcttg
      241 ctggcctttt ccgtgaacac ttcgaatgct ccgtcgttga atacgttgca attctgatag
      301 atctccacga acgaagcgcc cttgtgctcg ttcgcctgcc gcagcaccga ttgaaggtgc
      361 ttgggatcac ggtccatcga gcgcgccaca aaggtcgcat cggcacccat gcacaatgcg
      421 atcgggttga acggatggtc aaccgaaccc atcggcgtgg acttggtgat cttgccttcc
      481 tccgaggtcg gagaatactg gcccttggtc agaccgtaga tctcgttatt gaagagcaga
      541 acgtttacat tgaagttgcg gcgcaacagg tggatcgtat ggttaccgcc gatcgaaaga
      601 ccgtcgccgt cgccggtaac gatccagatg ctgagctccg gacgggccgc tttcaggcca
      661 ctggcgatcg ccgtcgcgcg accgtgaatg gagtgcatgc cgtacgtttc catgtagtaa
      721 gggaaacggg acgaacagcc gatgccggaa acgaacacga tgttctcgcg tggaatccca
      781 aggtcgggaa taaccgactg cgcctgcttc aggatcgagt agtcgccgca accgggacac
      841 caacggactt cctggtcggt gacgagttct ttcgacgtat atttttctat ggtggggtta
      901 ttattctcca tgatgatgac ttccttttta tgatcaaccg gtgattactt gagggtctcc
      961 tcgatcttct tgagaatctc ttccgcggtg aacggcatac cctggatctt gttgaatccg
     1021 accgccggca ccaggtactt gtcgcggatc atcttgatga gctggcccgt attcatctcc
     1081 gggatgatca cctggtcaaa cgcatacagc aattcgccca ggttcttcgg gaacggacgg
     1141 atatgacgga ggtgggcata ggatacgtcc agccccttct cacgcgcctg gagcaccgcg
     1201 ctcttgatgg cgccgtaggt ggagccccaa ccgagcatca agagcttacc cttggcatta
     1261 ccgatctcgg gtttgatctc cggaatatag tcggcgatct tttccacttt cgcttcgcgc
     1321 atacggacca tgaattcatg gttggctgcg tcgtaactga tgttaccggt ttcgtgctgt
     1381 ttctcgagac cgccgatgcg gtgctcgagt cccttggttc cgggaattgc ccaggggcgt
     1441 gcaagccgct cgtcgcgctt gtaggggagg aatttgtctc cgttgttttt ctccgtagca
     1501 aacttcaccg gaatggcagg aaggtctgcc gccttcggga atttccaggg ctcggcgccg
     1561 ttggcgatgt atccgtcggt caacaggatc accggcgtca tgtgttgcac ggcgatccgg
     1621 caagcctcat acgccatgtc gaagcaatcg gatggcgtcg cagcggcgat gaccggaatc
     1681 ggactctcac cgttccgtcc gtaaaccgcc tgcaagaggt cactctgctc ggtcttagtc
     1741 gggaggcccg tcgaaggacc tccgcgttgt acgttcacga tcaccagggg caactcgagc
     1801 atgagcgcaa gtcccatggc ttcggtcttg agcgcgatac cgggcccgga ggaagtcgtc
     1861 accgacaagg ctccgccgaa gctggcgccg atcgccgacg caatggctgc gatctcgtct
     1921 tccgcctgga aagtctttac gttataattc ttgtgccggg atagttcgtg cagaatatcc
     1981 gaagcagggg tgatcggata agaacccagg aacaagggca aaccgctctt ctccgcagcc
     2041 gcgatcaatc cgatcgcgac agcctggtta cccatgatgt tccggtaggt gccggaagcg
     2101 atcttggccg gattaatcgt gtagcgggtg gtgaactctt cccgcgtctc tccgaagttg
     2161 tagccggcct tcaacacttt cacgttcgcg tcgcggatct caggcttctt cgaaaacttg
     2221 tcttcgatga acttcagcgt atgctccatg ttccggttgt acatccagta caggaagccc
     2281 aacacgaaca tgatcttcgc tcggtcgatc tccttcacgc ccagtccgct tccttcgagc
     2341 gccgtacgcg tcatcttcgt tacatccaac ttgatcacac ggaaagaatc caccgtaccg
     2401 tcttccagcg gattcttgtc gtcgtatccg gcaagacgca gattcttcgg atcaaaaccg
     2461 tccgtattca cgatgatggt tccgctcttg cgcaggaagc gcaggttggc tttcaacgcg
     2521 gccgcgttca tcgcaaccag tacgtcacaa taatcacccg gcgtgaagat ctcgacactg
     2581 ccgaagtgta actggaatcc tgaaactccg gcaagcgtac cctgaggggc acggatctct
     2641 gccgggaagt cggggaaggt gctcaggtcg ttcccgaaca gcgccgcggt attggtgaac
     2701 tgggtacccg tcaactgcat cccgtcaccg gagtctccgg caaacttgat gatgacgttc
     2761 tccagcactt cattctgacg ttccatagat ggttggggag aagtagttga attcatacaa
     2821 atatagggat ttttcggcct tttcctggaa gccaaagatc cctgaaaagc cacacctgcc
     2881 acatgacagg ggtcacatcc agcccttttc gaacaggtcc cgggtatgat aggaaatgat
     2941 cacatccgcc ccggctcggg
//
LOCUS       DAOTON010000300         2952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26227_length_2952_cov_3.267863, whole genome shotgun sequence.
ACCESSION   DAOTON010000300 DAOTON010000000
VERSION     DAOTON010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26227_length_2952_cov_3.267863"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="P5292_06750"
     CDS             <1..384
                     /locus_tag="P5292_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS38863.1"
                     /translation="APIIPGINAPEIPTIIEAAAAAGALNAGMTIVRLNDAVADVFKS
                     WLHEHFPDRAEKVLNQIAECHGGSLSDSRFGTRMSGEGNIAESIKQLFRQSVRRHLAG
                     RSVPGYDLSAFRIPEERMQLRLDLF"
     gene            718..>2952
                     /locus_tag="P5292_06755"
     CDS             718..>2952
                     /locus_tag="P5292_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38864.1"
                     /translation="MIKKLLLVLVPVIAVTLLTSEQMSDNGKAAKTGAPGEVDCTDCH
                     SDFGINSGGGSISITATGMPTHEYTPGQTYNMSVTVARSANSLFGVGIEALTASNANG
                     GTLNITDATHTQIKSATVSGVSRRNVVHTLDGGANPSSMTFNFSWTAPAAGTGPVTFY
                     FAGVAADGDGNESNDYVYKSNQVFTEATCTTPAQPGSISGGATQCSGSAVTYSVATVS
                     GATSYTWTLPSGWTGSSTSNSINVTSGSTSGNVTVTANNACGSSTAQTLAVTANTTPA
                     QPGTISGGATPCSGSSVTYSVAAVSGATTYTWTLPSGWTGTSTSNSINVTTGSTSGNI
                     SVTANNSCGNSTARTLAVTASATPSQPGSISGSTSLCSGTATTYSVATVAGATTYTWT
                     LPSGWTGTSTTNSINVTSSTTSGNITVTANNSCGSSTPSTLAVTAGTMSVNSSSTSPA
                     CNGGTDGSATVTVSGGTGPYTYQWSNGNTTASVSGVAAGSYSVTVTDQTGCVNNLSVS
                     ITEPSAIVADAGANAAVCSGAAATIGGSPTGLGGTGTLAYLWSPATGLSSATDPNPVA
                     TPASATGYTVVVTDANGCSSSATVQVDIDATPAPVITMSGDTLYTSGGVTYEWYLNGN
                     LAGSGASPFFVATQSGNYEVVAYSAAGCAGVSPVFAFTPTGIYSSALLAGINVFPNPA
                     RDEFQITLPAGSGSVVARLMDLTGKEVLLTTIDEQHRTVDVRMLQSGVYFLTLELNGQ
                     RAMSR"
BASE COUNT          645 a          871 c          787 g          649 t
ORIGIN      
        1 gcgccgatca ttcccggaat caatgcaccg gagatcccca ccatcatcga agcggcagcg
       61 gcagcaggag ccctcaacgc ggggatgacg atcgtccgcc tcaacgatgc ggttgcggac
      121 gtattcaagt cctggctgca cgaacacttt cctgacaggg cggagaaagt gctgaaccag
      181 atcgcggaat gtcacggagg ttctctgtcc gatagccggt ttggaacccg catgtccggc
      241 gaaggaaata tcgccgagag catcaagcag ctctttcgac aatcggtccg ccggcatctg
      301 gccggcagat cagtgcctgg ttatgatctg tccgccttcc ggattccgga ggaacggatg
      361 caattgcgac tcgatctgtt ttaatcacga agtaccctta ctgttagtga tagttttact
      421 attgaacttt ttattaaaag caggtgctgg aatttggagc ttagcatgaa cagggaaacg
      481 cgcttcccgc gctgattgcg actttcctga ggttttacaa gaacttcggt agggaagatg
      541 aataacattc ctgaagtgac atccatcact tttcggagtg ttttccttca gctactttgc
      601 gccagcttcg atggtgtttg aagttcccct ctgattcgga accattgaat gtgaccaact
      661 gcgaccggct acgtccggtg tccattcaat tcatcactta tctcaaccct caccaccatg
      721 atcaaaaaac tattactggt tctcgttccg gtaatcgcgg tcacactgct gaccagcgaa
      781 caaatgagcg acaacggcaa agcagctaaa accggcgccc cgggtgaagt tgattgtacg
      841 gattgtcaca gtgacttcgg catcaactcc ggcggaggat cgatttcgat caccgctacc
      901 ggtatgccga cccatgagta tacaccgggc caaacctata atatgtcggt taccgtcgcc
      961 cggtcggcca acagtttgtt cggtgtcggc atcgaagcac tgacggcttc gaatgccaac
     1021 ggcggcacgc tgaatatcac ggatgctacg catactcaga tcaaatccgc gactgtcagc
     1081 ggggtctccc gtcgtaacgt ggtacatacg ctcgatggcg gtgctaaccc cagctcgatg
     1141 acctttaact tcagctggac agctcctgcg gctggtactg gtccggtcac cttctacttc
     1201 gccggcgttg ccgccgacgg tgatggtaac gagagcaacg actacgtgta caaatccaac
     1261 caggttttca cggaagctac ttgtacgaca ccggcacaac cgggctcgat cagtggcggt
     1321 gccacgcagt gcagtggttc ggctgtgacc tattcggtgg ctacggtgtc cggcgcaact
     1381 tcttatacgt ggacgcttcc ttccggatgg acgggaagtt cgaccagcaa cagcatcaac
     1441 gttacctccg gcagtacctc cggtaatgtt accgttactg ccaataatgc ctgcggtagc
     1501 agcaccgcgc aaacactggc cgtaaccgct aacaccacgc cggctcaacc gggtacgatc
     1561 agtggtggcg caaccccctg cagcggaagt tcggttacct attccgtagc ggcagtttcc
     1621 ggcgcgacca cgtatacctg gacattgcct tccggatgga cgggaacctc gaccagcaat
     1681 agcatcaacg ttaccaccgg aagtacctcc ggcaatatct ccgtaacggc gaacaattcg
     1741 tgtggaaaca gtacggctcg caccctggcc gtaacggcta gcgctacgcc ctctcaaccg
     1801 ggatctatca gtggtagcac ttccttgtgt tccggaaccg ctaccaccta ttcggtagca
     1861 acggtagcgg gcgccacgac ctacacctgg acgttgccgt ccggttggac cggtacgtct
     1921 acgacgaatt ccatcaacgt cacctccagc acgacttccg gtaacatcac ggtgaccgcc
     1981 aataattcct gcggaagcag tacacccagt accctggccg ttaccgccgg cacgatgtcc
     2041 gtcaacagtt catccacctc accggcttgc aacggtggaa ccgatggttc cgccactgtc
     2101 acggtttccg gtggtaccgg accctacacg tatcagtggt ccaacggtaa cacgactgcc
     2161 agcgtgagcg gcgtcgctgc cggtagttac agtgtaacgg ttaccgacca aaccggatgt
     2221 gtcaacaacc tgagcgtttc aatcacggag ccttcggcta tcgttgcaga cgcgggtgcc
     2281 aatgcggcag tttgctccgg cgctgcagct acgatcggtg gaagtccgac aggcctgggt
     2341 ggcaccggca cgcttgctta tctgtggtcc cccgctacag gtctgagctc ggccactgac
     2401 ccgaacccgg tcgcgactcc cgcgtcggct accgggtaca cggttgtagt cacggatgca
     2461 aacggatgca gctcatcggc aaccgtacag gtcgacattg acgcgacgcc cgcgccggtg
     2521 attaccatga gcggcgatac cttgtatacg agtggtggtg taacctacga atggtacctc
     2581 aacggcaatc tcgcaggttc aggtgcttct ccgttcttcg tcgccacgca gagtggtaac
     2641 tatgaagtgg tggcatattc agcagcaggt tgcgccggcg tttctccggt ctttgcgttc
     2701 accccaacgg gtatttattc atccgcactg cttgccggaa tcaatgtttt cccgaatccc
     2761 gcccgcgatg agttccagat cacacttccg gccggaagtg gttcggtagt cgcacggctg
     2821 atggacctca ccggaaaaga agtgttgctt acgactatcg acgaacaaca tcgtacggtc
     2881 gatgtgcgca tgcttcaaag cggcgtttac ttcctcaccc ttgagctgaa tggtcaacga
     2941 gccatgagcc ga
//
LOCUS       DAOTON010000301         2940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26368_length_2940_cov_2.800693, whole genome shotgun sequence.
ACCESSION   DAOTON010000301 DAOTON010000000
VERSION     DAOTON010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26368_length_2940_cov_2.800693"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="P5292_06760"
     CDS             complement(<1..1571)
                     /locus_tag="P5292_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38865.1"
                     /translation="MMLRLLSLSFLLLLHSCSFRAKAQAGCGNDSVVVTWVYGLNQAM
                     GNYTYAVSTATYDLDSGIVETCTQLDFPPSPPNCNTGYWSRTDTTFDGSGRVVLLLTL
                     TGSPTGWVNDRQTIRSYHPVYNQLVFEAKLQWNGSAWDSVEVTDRIVNGSGQVTETAV
                     YDFSGSSRVPRTRELVTYIDGHPNDRTYQHGDLGNWVNDSLYQFSYSGTIRTGLAYST
                     WDSGASNWVFQSLTPYLFQTDEWCAERKRYDDAVVSGIQTLDSVTWYVDTLEQTVYYH
                     SFAWTDFNLSGSFFPTAYIKDFDSGYLRGELLLIHSAEGDYALEVISGDSTWFAVDRP
                     VVTDYTYDTLNHYSYIESTGGCTNPCGSEFTYNIEPDGFQASQHFHQWTMTNDEDIYT
                     IAERGPRNRPMLVAPIWEYDVPLCYNETYQPHITLAGGCAPYSVRWTPSNGLSNDTIL
                     DPVITVRAYQAYTLVVTDANGQTDTLQYSLSPVIDMSLQQPATVCDTLRTLELYNGAG
                     LTTQWYWNSVPIPDAT"
     gene            1669..1896
                     /locus_tag="P5292_06765"
     CDS             1669..1896
                     /locus_tag="P5292_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38866.1"
                     /translation="MGEGIDQERYALPRSPAVPLSARRRINAADPLEPSISIHHYPLR
                     SSGMAADLHRGYGTALFLHLNLAPDCSLATP"
     gene            2046..>2940
                     /locus_tag="P5292_06770"
     CDS             2046..>2940
                     /locus_tag="P5292_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS38867.1"
                     /translation="MISRTLSAKLPHTFFRGKALILLGPRQVGKTTLIRHLLLKEAHL
                     FLNGDDPAIRNLLSGVSESRLKTIIGRYHFVYIDEAQRIPDIGLTLKLITDRFKAVQL
                     LVSGSSALEINLTTQEPLTGRKIEYRLFPLSWEELESSLGYLEAEQQLEERLVFGMYP
                     EVVTKRDNAREVLQELCTSYLYKDVLAISGIRKPEILEKLLRALALQLGQEVSYNELS
                     GLIGIDKNTVARYIELLEKTFIVFRLNSFSRNLRNEIRENRKIYFYDNGIRNAIIQNL
                     NPLSLRNDKGALWENFLVTERM"
BASE COUNT          745 a          856 c          734 g          605 t
ORIGIN      
        1 gtggcatcgg gtatcggcac actgttccaa taccactgcg ttgtcagacc ggcgccattg
       61 tacaactcaa gcgtgcgcag ggtgtcgcaa acagtggcgg gttgctgcaa cgacatatcg
      121 atgacgggtg agagcgaata ctgcaaagta tcggtctgcc cgttcgcgtc ggtcaccaca
      181 agggtgtaag cctgataagc cctcacggtt atcacgggat ccaggatggt gtcgttcgag
      241 agaccgttcg aaggtgtcca gcgcaccgaa taaggtgcac agccaccagc cagcgtgatg
      301 tgcggctgat aggtctcgtt gtaacacaac ggcacatcgt actcccagat cggagcgacc
      361 agcatcggcc ggttacgcgg accccgctcg gcgatggtat aaatgtcctc atcgttggtc
      421 atcgtccact gatgaaaatg ctgcgaagcc tggaatccgt ccggctcgat gttgtacgtg
      481 aactcactac cgcaaggatt cgtacagccg ccggtggact cgatatacga atagtgattg
      541 agcgtatcgt aagtataatc ggtcaccacc ggacgatcaa ctgcaaacca ggtcgagtcg
      601 ccggagatca cctccagcgc gtaatcgccc tcggcagaat ggatcagcaa caactcacca
      661 cgcagataac cggagtcgaa atccttgatg taagccgtcg gaaagaatga acccgacaaa
      721 ttgaaatccg tccaggcgaa ggagtgataa tagaccgttt gctccagcgt atccacgtac
      781 cacgtcacgc tgtccagggt ctggataccg gacaccacag catcatcgta ccgctttcgt
      841 tcagcgcacc actcatccgt ctggaagagg tagggtgtca gcgactggaa tacccagttg
      901 gaagctccgg aatcccaggt actgtaagca agaccggtac ggatcgttcc cgagtaactg
      961 aactgataca acgagtcatt cacccagttg cccaggtcgc catgctggta ggtacggtcg
     1021 ttcggatgcc cgtcgatgta ggtcaccaac tcgcgcgtgc gcggcacccg actgctgcca
     1081 ctgaaatcgt aaacggctgt ttccgtaacc tgaccagatc cattgacgat ccggtcggtt
     1141 acttcgacag agtcccaggc cgaaccattc cattgcaact tcgcctcgaa taccaactgg
     1201 ttgtaaacgg gatgatagga gcgaatcgtc tggcggtcat tcacccaacc ggtcggagat
     1261 ccggtcagcg tcagtaacaa aaccacacgg ccggagccgt cgaacgtggt atcggtgcgc
     1321 gaccaatagc cggtattaca attcggtggc gagggaggaa agtcgagttg cgtacaggtc
     1381 tccacgatcc ccgagtccag atcataggtc gcggtggaaa cggcataggt ataattgccc
     1441 attgcctggt tcaggccata cacccaggta acgaccaccg agtcgttgcc acacccggct
     1501 tgcgcctttg cccgaaacga gcatgaatgc aacagcaata aaaaactgag agaaagtaag
     1561 cggagcatca tgtggagatg ataagacaaa gcaagatggt caaatcccga cgcttcgaca
     1621 aaaccctcca acgctgctgc cctgcttttt taccaacgca tagtatgcat gggtgaagga
     1681 attgatcagg agcggtacgc cctgccccga tcacccgcgg tcccgctctc ggcccgccgc
     1741 cgcatcaacg ctgccgaccc gcttgaacca tcgatctcca ttcaccacta cccgctccga
     1801 agttccggca tggcggcgga cctccatcgg ggctatggca cagcactttt tcttcacctc
     1861 aacctcgctc cggattgtag tttagccaca ccttgaaaaa tgtaggatat tcgtcaaaaa
     1921 aatcaaattt tacgccctct atccttctat tcaccaaata tactatatat ttggtgaacg
     1981 tattcaccaa atatagcata attttggtga ttatattcac caaaattatg ctatatttgg
     2041 tgattatgat ttccagaaca ctttcggcca aactcccgca tacgttcttc cggggtaaag
     2101 cgctgatcct gctcggccct cgccaggtcg gaaagaccac cctcatcagg catctgctgc
     2161 taaaggaggc ccatcttttc ctcaacggcg acgaccccgc gatccggaac ctgctgagtg
     2221 gtgtaagtga atcgcgcctt aaaaccatca tcggccgcta ccatttcgtt tacatcgacg
     2281 aagcccagcg cattcccgat atcggactta cgctcaaact gatcaccgac cggttcaagg
     2341 ccgtacaact gctcgtcagt ggttcctcgg cattggaaat caacctgacc acgcaggaac
     2401 ccctcaccgg tcgtaagatc gaataccgcc tcttcccgct cagttgggaa gaacttgaat
     2461 caagcctcgg atatctcgaa gcggaacagc aattggaaga acgactcgta ttcggaatgt
     2521 atccggaagt ggtcacgaag cgcgataacg cgcgcgaagt cctgcaggaa ctgtgcacca
     2581 gctacctcta caaagacgtg ctcgcgatct cgggcatccg aaagccggag atactggaaa
     2641 aactcctgcg cgcgctggcc ctgcaactcg gacaggaagt atcgtacaac gaactatccg
     2701 gcctcatcgg catcgacaag aacacggtcg cccgttacat cgaactgctg gaaaaaacgt
     2761 tcatcgtctt tcgcctgaac agtttcagca ggaacctgcg caacgagatc cgcgaaaacc
     2821 ggaagatcta cttctacgac aacggcatcc gcaatgccat catccaaaac ctgaacccgc
     2881 tttccctccg caacgacaag ggcgcgctct gggagaactt cctcgtcacc gaacgcatga
//
LOCUS       DAOTON010000302         2933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26453_length_2933_cov_3.699792, whole genome shotgun sequence.
ACCESSION   DAOTON010000302 DAOTON010000000
VERSION     DAOTON010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26453_length_2933_cov_3.699792"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..710)
                     /locus_tag="P5292_06775"
     CDS             complement(180..710)
                     /locus_tag="P5292_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38868.1"
                     /translation="MKTFIAKSTDGMMQPTIRQNMKQKIAGNRFFVYALELVLLYVLV
                     FAAVSAFAQEPATASKEPGKLEFAKETIVYNSGKVYMNWVAKAHSEDCIYVIERSVDG
                     QEFEPVGLKEGIGSPLELLYSWVDTKPVAGTVHYRIKQINNEGNLVAQAEPKTVTSPD
                     ASPLFMDKSSRMVQVK"
     gene            996..2453
                     /locus_tag="P5292_06780"
     CDS             996..2453
                     /locus_tag="P5292_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38869.1"
                     /translation="MDTLNKTIKKLSDGEYQELLSAVAGRKNNKPYLVLEAARNGSYT
                     ESQMMDMLGVNPSTYYTLKSRLNERIAQYLSTNVKNPISALKDKVALVPAMLFSNDRD
                     VAIRALKNLEKQLIEYDLSNELIVVYKALARLSMYNGDFDYYEKEYNRHVAYSLAVVK
                     AEDLFYEFVKRAGSYQLTRDERDFEAVQSILRELTNISELYHGHRLFVLYNLVRIYYL
                     CLFTSRVETLKALEIEVDSILQQIRKHFEKYELDTFYQGVRFLVDFLYFEYYQRTGNQ
                     VRADHYYSMINKQVPDIAFKPAFSFYVTEFLNSKVARFLATRDTQQLLDLNAELESNF
                     DVDVNEPYPYISFQRFNAIGKFYQQDFAGAARIINNLRNETSLKKFLFTDVEYKLFQA
                     MQYCFQGEDGLCNQLLQSVKRQIVENDNNYQPALHLIKMLKAAIKPEEFRKKVKKLTE
                     LNEQFRQVNQGAHRMFWYIQLDEQMIRRMANPIKD"
     gene            2725..>2933
                     /locus_tag="P5292_06785"
     CDS             2725..>2933
                     /locus_tag="P5292_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRS38870.1"
                     /translation="MNKDVTQFLVYQYAEQLSDVFWEEYDKWPKKVQMTIGLQIIRAA
                     DSISANIAEGFGRFHYRDRLRFYYCA"
BASE COUNT          712 a          783 c          715 g          723 t
ORIGIN      
        1 aaaaggaggc taccggtacc gcgcccgccc gaaggacggc cgacagtgtc agcagtatca
       61 ggaatcccgc catccgtccc aggcattcac cccagcctgt ggtcggttgg tttcccattg
      121 tcttgtttga acgatcttac accctaaaca cccttcgagt gcccttacgg gcaggtcaat
      181 tacttgacct gcaccatacg gctgctctta tccatgaaga gcggactggc atccggagac
      241 gtcaccgttt tcggctcggc ctgggctacc aggttccctt cattattgat ctgtttgatg
      301 cggtagtgca ccgtgccggc aaccggcttg gtgtcgaccc agctatagag gagttccagc
      361 ggggatccga taccttcttt aaggcctacc ggttcgaact cctgtccatc gacggagcgt
      421 tcgatgacgt agatgcagtc ttccgaatgg gcttttgcca cccagttcat gtagaccttg
      481 cccgaattat agacaatggt ctccttcgcg aactccagct tacccggctc tttcgaggcg
      541 gtggcgggtt cctgtgcgaa ggcactgact gccgcgaaca ccagtacata taggagtacc
      601 agttcgaggg cgtacacgaa gaagcggttg cccgcgatct tctgtttcat gttctgtcgg
      661 atcgtgggct gcatcatgcc gtccgttgat ttcgcgataa aagttttcat cgtgagagct
      721 tttgaggatt ctagtgacaa tatatactga gtggctcccc ggcagttccc gcaataagcg
      781 gcgcatctta cggatgtaag gttttgtccg gttccgctgc attttcctcc agatccggct
      841 tatagtcttt gaaaaaacgg agaatttgcc cttttaacct tttaagaccc ttgcaaaccc
      901 aagccctcaa ttcctcttga ctccggtgta tccttctccg gtcacccccg ttaaacctct
      961 tcagaacaaa cgctccacag ctattacagc gcaccatgga tactttgaac aaaacgatca
     1021 agaaactgag tgatggcgag tatcaggaac tgctttccgc ggttgccggc cgaaagaaca
     1081 acaagcctta tctggtattg gaagccgcgc gaaacggcag ctatactgaa agccagatga
     1141 tggatatgct gggtgtgaac ccgagtacct attatacgct caagtcgcgc ctgaacgaac
     1201 gcatcgcaca gtatttgtcg acgaacgtca agaacccgat ctccgcgctg aaagacaagg
     1261 tggccctcgt gccggccatg ttgttcagca acgaccgcga tgtggccatt cgtgcgctca
     1321 agaacctcga aaagcagctg atcgaatatg atctctcgaa tgaattgatc gtcgtttaca
     1381 aagcgctggc acgcctgagc atgtataacg gcgatttcga ttattacgag aaagaataca
     1441 accgtcacgt agcgtactcc cttgcggtgg tgaaggcgga agatctcttc tacgaattcg
     1501 tgaagcgtgc cggcagctac cagctgaccc gcgacgagcg cgatttcgaa gcggtgcagt
     1561 ccatccttcg ggagttgacc aacatctccg aactgtatca cggacaccgc ctgttcgtgc
     1621 tctacaacct ggtgcgcatt tactacctct gtctgttcac cagccgggtg gagacattga
     1681 aagcgctcga gatcgaggtg gattccatcc tccagcagat tcggaagcac tttgagaaat
     1741 acgagctcga taccttttat cagggcgtac gattcctcgt cgacttcctg tacttcgaat
     1801 attaccagcg taccggaaac caggtccgcg ccgatcatta ctattcaatg atcaacaagc
     1861 aggtgccgga catcgctttc aagccggctt tcagcttcta tgtgaccgaa ttcctgaaca
     1921 gcaaagtcgc ccgcttcctc gcaacccgcg atacgcagca attgctggac cttaacgccg
     1981 agctcgaatc gaacttcgac gttgatgtga acgaacccta tccgtacatc agcttccagc
     2041 gtttcaatgc catcggtaag ttctaccagc aggatttcgc cggggctgcg cggatcatca
     2101 acaacctccg caacgagacc agcctgaaga aattcctgtt caccgacgtg gagtacaagc
     2161 tcttccaggc catgcagtac tgcttccagg gagaagacgg actgtgcaac cagctcctcc
     2221 agagcgtgaa gcgccagatc gtcgaaaacg acaacaatta ccagcctgcc ctgcacctga
     2281 tcaagatgct gaaggcggct atcaaacccg aagaattccg taagaaggtg aaaaaactca
     2341 ctgagctgaa cgagcagttc cgccaggtta atcagggtgc gcaccgaatg ttctggtaca
     2401 tccagctcga cgagcagatg atccgtcgca tggcgaaccc gatcaaagac tgatttttct
     2461 tttgccccgc caggttatgc ctcccggaga acttcgccgg gaggcatttt tttttgtaac
     2521 tggtaattgg taactagtaa ctagtaacta aaaactggta actgataacc ggtaacgaat
     2581 tactagtttt caattactag ttaccaatta ctagttacca attaccagtt accagtttcc
     2641 atttcacgaa aaatatttca ttccgattcc ttttcccgct tttatgcggt tgacatccgg
     2701 cataattcaa atttactttg ttgggtgaat aaggatgtga cgcagtttct ggtctatcag
     2761 tacgccgagc agttgtcgga tgtgttttgg gaagagtatg ataagtggcc taagaaagtt
     2821 caaatgacga ttggtttaca gatcatccgg gcggcggata gtataagcgc aaacattgcg
     2881 gagggcttcg gaaggtttca ctatagggac cgcttacgat tttattattg tgc
//
LOCUS       DAOTON010000303         2932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26466_length_2932_cov_3.282586, whole genome shotgun sequence.
ACCESSION   DAOTON010000303 DAOTON010000000
VERSION     DAOTON010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26466_length_2932_cov_3.282586"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="P5292_06790"
     CDS             complement(<1..239)
                     /locus_tag="P5292_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38871.1"
                     /translation="MKLLPEVKKVKYPQLDALRFFSVAGVMISHWFSQFAFTEYIPFG
                     DGVLLFFVLSGYLIGNILLEQKESIEVAGDKLSAL"
     gene            complement(223..495)
                     /locus_tag="P5292_06795"
     CDS             complement(223..495)
                     /locus_tag="P5292_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38872.1"
                     /translation="MKAPTSSHILNTSSNLLGFCLVVLTSLRIANYSEQSLIDEFTVV
                     ASVLLMAASILSFLAIRSDFAFHSNHKYLPHLKFRNLAPHFYETIA"
     gene            complement(545..1855)
                     /gene="murF"
                     /locus_tag="P5292_06800"
     CDS             complement(545..1855)
                     /gene="murF"
                     /locus_tag="P5292_06800"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRS38873.1"
                     /translation="MSIPLEALYKYYRQHPVVCTDTRTIVPGCLFFALKGPNFNGNAF
                     AAKALASGAAFAVVDEPGAVTDPRCLLVEDVLTSLQLLAQHHRRQLRIPFLAITGSNG
                     KTTTKELVRAVLAKKFKTLATKGNLNNHIGVPLTILSITDEIEFAVIEMGANHQQEIA
                     SYCKIVEPDYGLITNVGKAHLEGFGGFEGVKKGKGELYEWIGAHGKLVFLNNDNPHLT
                     EMVAVRGAEHRQRYGTTADCDCRGELLGELPGLHLRWTYGTVAGEIKAKIVGRYNFEN
                     VLAAICIGNYFGVSAEQIGAAIEAYEPDNSRSQLVHRGSNTIVLDAYNANPTSMEAAL
                     RNFEAIESDRKVVCLGEMAELGDASEEEHMRLAERLRLANFSNIILVGARFATWAGRL
                     SCMHFDDSEKAAAWMRQQRFEQTWFLVKGSRSTKMEKVAEAIAG"
     gene            2126..>2932
                     /locus_tag="P5292_06805"
     CDS             2126..>2932
                     /locus_tag="P5292_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRS38874.1"
                     /translation="MANIGKITQIIGPVVDVSFEAEGATLPNILDALEIERPGGALVM
                     EVQQHIGEDTVRAIAMDSTDGLTRGTPVRAMGAPIRMPAGDAIKGRLFNVVGDAIDGI
                     GSVSKDGGVPIHRTPPRFEDLSTESEVLFTGIKVIDLIEPYAKGGKIGLFGGAGVGKT
                     VLIMELINNIAKSYSGLSVFAGVGERTREGNDLLREMIESGVIRYGEEFKHSMEKGGW
                     DLGKVDRTELAKSQATLVFGQMNEPPGARARVALSGLTVAEYFRDGDEKSG"
BASE COUNT          694 a          843 c          785 g          610 t
ORIGIN      
        1 ataagtgcac ttaatttatc gccggccacc tcaatgcttt ctttttgttc gagaagaatg
       61 ttgccaatta aatagccact caacacaaag aagaggagaa ctccatcacc aaatggaatg
      121 tattcggtaa aagcaaattg agaaaaccaa tgactaatca ttacccccgc caccgaaaaa
      181 aagcgcaatg catctaattg cggatacttc acttttttta cttcaggcaa tagtttcata
      241 gaaatgcgga gcgagatttc gaaatttcag atgcggaagg tatttatgat tagagtggaa
      301 agcaaaatcc gaccggatcg ccaggaacga caggatgctg gcggccatca acagtaccga
      361 ggctacgacc gtgaattcgt cgatcagcga ttgctccgag tagttggcga tccggaggga
      421 agtcaggacc accaggcaaa agcccaggag gttcgacgaa gtattgagga tgtgcgagga
      481 ggtgggagct ttcatgtttt acacgaccaa aattgatcct gcacatgaag ttgcagtgac
      541 gcactcaacc ggcgatcgct tccgccacct tttccatctt cgtactgcgg gaacccttta
      601 cgagaaacca ggtctgttcg aagcgttgct gacgcatcca ggccgcggcc ttttcggaat
      661 cgtcgaaatg catgcaactc agtcggccgg cccaggtggc gaagcgcgcg ccgaccagga
      721 tgatgttgct aaaattcgcg aggcgcagtc gctccgccag ccgcatgtgt tcttcctcgg
      781 aagcgtcacc caattccgcc atttcgccga ggcagacgac tttccgatct gactcaatcg
      841 cttcgaaatt ccggagcgcg gcttccatgc tcgtggggtt cgcgttgtag gcgtcgagca
      901 cgatggtgtt gctgccgcgg tggaccaact gcgaccgact gttgtccggt tcgtaagcct
      961 cgatagccgc cccgatctgt tccgccgaaa cgccgaagta attcccgatg cagatcgcgg
     1021 ccaatacgtt ctcgaaattg tatcgaccga cgatcttcgc tttgatctct cctgccaccg
     1081 taccatacgt ccagcgcaag tggagccccg gcaattcgcc caacaactcg ccccggcagt
     1141 cgcaatcggc cgtagtgccg taacgctgtc ggtgctcggc gccgcgtacg gcgaccattt
     1201 ccgtcaggtg tggattgtcg ttgttcagga aaacgagctt gccgtgcgcg ccgatccatt
     1261 cgtacaactc gcctttgcct ttcttcacgc cttcgaatcc gccgaagcct tcaagatgcg
     1321 ccttacccac gttcgtgatg aggccgtagt ccggctccac gatcttgcaa tacgacgcga
     1381 tctcctgctg atgattggcg cccatttcga tgaccgcgaa ttcgatttca tccgtgatgg
     1441 acaagatggt cagcggcacg ccgatgtgat tgttcaggtt tcctttcgtc gcgagtgtct
     1501 tgaacttctt cgcgagtacc gcgcgcacca gttccttcgt cgtcgtcttc ccgttggaac
     1561 cggtaatggc caggaacgga atacgcagct gccggcgatg gtgctgcgcc agcaattgca
     1621 ggctggtcag cacatcctcc accagcagac aacgcgggtc cgtgaccgcg ccgggctcat
     1681 cgaccaccgc aaacgcggcg ccggaagcaa gcgcttttgc agcgaaagcg ttcccgttga
     1741 agttcggtcc cttgagggcg aaaaaaaggc aaccaggcac aatcgtccgg gtatccgtgc
     1801 agacaacagg atgttgccgg tagtacttgt agagtgcttc gagcgggatc gacattttgc
     1861 caaaaataac gggcttgtaa ccccaaatcg tgcaggaatc ggtatttcat ccacgacccg
     1921 ctgtagagaa gcctgaaaca cgaaagctac cgggttcaaa ttcaagccct ggaaaggaat
     1981 cgtaaaacaa ctgtaaaata ctgaatttca atactccagg aggcgttgag gagtatagaa
     2041 acaaattacc aacaggctac cggattcgcc cgaaaaacgc tacttttgcg ccttcaaaac
     2101 gcaaaccaaa cgcatctcca taaccatggc aaacattggt aaaatcaccc agatcatcgg
     2161 tccggtagtg gacgtgagct tcgaagccga aggcgcgacc ctgccgaaca tcctcgacgc
     2221 actcgagatc gaacgcccgg gcggcgcact cgtgatggaa gtgcagcagc acatcgggga
     2281 agacaccgtc cgcgcgatcg cgatggactc caccgacggt ctgacccgcg gaactccggt
     2341 tcgcgccatg ggcgccccga tccggatgcc ggccggtgat gccatcaaag gccgcctgtt
     2401 caacgtcgtt ggcgatgcca tcgacggtat cggttccgtt tccaaagacg gcggcgttcc
     2461 gatccaccgc acccctcccc gcttcgagga cctctccacc gaatcagaag tactgttcac
     2521 cggtatcaaa gtcatcgacc tcatcgaacc ttacgccaaa ggcggtaaga tcggtctgtt
     2581 cggcggcgca ggtgtaggca agacggttct gatcatggaa ctgatcaaca acatcgccaa
     2641 gtcgtactcc ggtctttccg tattcgccgg cgtaggcgag cgtacccgtg aaggaaacga
     2701 cctcctccgt gaaatgatcg agtcgggcgt tatccgctac ggtgaagagt tcaagcacag
     2761 catggaaaaa ggcggctggg acctcggcaa agtggaccgc acggaactgg ccaagtcgca
     2821 agccaccctc gtgttcggtc agatgaacga acctccggga gcccgtgcac gcgtggcact
     2881 gtccggtctg accgttgctg aatacttccg cgacggagac gagaaatccg gt
//
LOCUS       DAOTON010000304         2931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26474_length_2931_cov_3.315716, whole genome shotgun sequence.
ACCESSION   DAOTON010000304 DAOTON010000000
VERSION     DAOTON010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26474_length_2931_cov_3.315716"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1694
                     /locus_tag="P5292_06810"
     CDS             <1..1694
                     /locus_tag="P5292_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS38875.1"
                     /translation="AQVVRKTLDEVTGALHQVESLDASGKSAVLDTVTGQVFRFFLLI
                     LGVALLRGALMFLMRQTIIVMSRHIEYDLKNEVFDHYQRLSLSFYRRNNTGDLMNRIS
                     EDVSRVRMYVGPAVMYAINLMVMFVLVIISMWHVNHTLTLYVVAPLPLLSLSIYFVSD
                     IMNRRSEEVQTQQSRLSTFVQEAFSGIRVLKSFVREKSSVETFRNESDAYLRHNMHLV
                     RVNAMFFPLMLLLVGLSTLLVIYVGGREVIAGRATTGNIAEFIIYVNMLTWPVASLGW
                     VTSLVQRAAASQTRLNEFMLQKPEIVSTVTEQREMKGAITFRNVSFVYPDSGVQALQD
                     VSFAVAPGRSLAILGKTGSGKSTIANLLARLYDPSKGSILIDDTDLRAWPLDALRRQI
                     GYVPQDVFLFSDTIANNISFGLEGNEDPVETRRRVETAARNAAVEENILEFPDRFETM
                     LGERGITLSGGQKQRVSIARAIIREPRILVFDDCLSAVDTQTEERILRNLREVMKDRT
                     TLFISHRVSTVRNADHILVLDNGKILEEGTHESLLKKRGAYYDLYESQLLEEHLN"
     gene            complement(1752..>2931)
                     /gene="dnaB"
                     /locus_tag="P5292_06815"
     CDS             complement(1752..>2931)
                     /gene="dnaB"
                     /locus_tag="P5292_06815"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRS38876.1"
                     /translation="VASAANIEYHARIILQKHIQRELIRISSETIRDAYEDTTDVFHL
                     LDRAEKNLFDIAQGNIRRNFQEMSAMVSEATRLIEAAKKHGTGITGVESGFTDLDRLT
                     SGWQKSDLIILAARPAMGKTAFVLSLARNAAIQFNKPIAVFSLEMSSIQLVQRMISSE
                     TGIPSDKLRKGTLDETEWQKLVSMTGRLSESPIFIDDTPALSVFDLRSKCRRLKAMHD
                     VQMIIIDYLQLMRADVENKGGNREQEISTISRSLKAIAKELNVPIIALSQLSRAVETR
                     GGSKRPQLSDLRESGAIEQDADMVMFIYRPEYYGLTHDEDNNPTTGVAEIIVAKHRNG
                     ATDTVKLRFVDRLAKFTDLDQRDFGDSSPAPSGSVVDYQSNRIIVGSRMNDMPEEPPF
                     "
BASE COUNT          632 a          833 c          822 g          644 t
ORIGIN      
        1 ctgcccaggt ggtgcgaaag accctggatg aggtgacggg ggcgcttcat caggtggaat
       61 ccctggatgc cagcggaaaa agcgcggtat tggatacggt taccggtcag gtcttccggt
      121 tctttctgct gatcctgggc gtggcgctgc tccggggcgc cctcatgttc ctgatgcgcc
      181 agaccatcat tgtcatgtcc cggcacattg agtacgacct gaaaaacgaa gtgttcgacc
      241 actatcaacg gctctcgctc tccttctatc gccgcaacaa taccggtgac ctgatgaacc
      301 ggatcagcga agacgtcagc cgggtacgca tgtatgtcgg gcccgcggtc atgtacgcga
      361 tcaacctcat ggtcatgttc gtgctcgtga tcatcagtat gtggcacgtg aatcatacgc
      421 tgaccctcta cgtcgtggct cccctcccgc tgctttcgct gagcatctac ttcgtcagcg
      481 acatcatgaa ccgccgcagc gaagaggtcc agacacaaca gagcaggctg agtacgttcg
      541 tgcaggaagc gttcagtgga attcgggtac tcaaatcatt cgtacgggaa aaatcatccg
      601 tcgagacctt tcgtaatgaa agtgacgcgt acctgcggca caacatgcac ctcgtgcggg
      661 tgaatgcgat gttttttcca ctcatgctct tgctggtggg cctgagcacg cttttggtca
      721 tttacgtcgg tggtcgcgag gtgatcgccg gaagggcgac aaccggaaac attgccgaat
      781 tcatcatcta cgtcaacatg ctgacctggc cggtggcttc actgggatgg gtgacctcgc
      841 tggtgcaacg cgctgcggct tcacagaccc gactcaacga attcatgttg caaaaaccgg
      901 agattgtcag cacggttacg gaacagcgcg aaatgaaagg cgcgatcacg ttcagaaacg
      961 tttcgttcgt ctatccggat tccggcgtac aggctttgca agacgtgagt ttcgccgtag
     1021 cgcccggccg atcgctggcg atcctcggca agaccggtag cggtaagtcc accattgcca
     1081 acctgcttgc tcgcctctac gatccttcga aggggagcat cctgatcgac gataccgacc
     1141 tgcgcgcatg gccgttggac gcactgcgcc ggcagatcgg ctacgttccg caagacgtgt
     1201 tccttttcag cgacacgatt gccaacaaca tctcgttcgg attggaagga aacgaagatc
     1261 cggtcgaaac acgtcgtcgt gtggaaaccg ccgcacgcaa cgctgccgtg gaagaaaaca
     1321 tcctggagtt tcccgaccgg ttcgaaacca tgctcggcga acgcggcatc accttgtcag
     1381 gcggtcaaaa gcaacgggtc tccatcgccc gtgcgatcat tcgtgaaccc cggatcctcg
     1441 tattcgacga ttgcctcagc gcggtcgata cccaaacgga agaacggatc ctccgcaacc
     1501 tgcgcgaggt aatgaaagac cggaccaccc tcttcatttc ccatcgcgta agcacggtaa
     1561 ggaatgccga tcacatcctg gtcctggaca acggtaagat cctggaagaa gggacgcacg
     1621 aaagcctgct gaaaaaacgc ggagcctatt acgacctcta cgaaagtcag ttgctggaag
     1681 agcacctgaa ttgaattatc ctcacgccaa aagaggcgtt cgggtgacgt ccggtaaaca
     1741 atcagaattt atcagaaggg cggctcttcc ggcatatcgt tcatccgtga ccccacgatg
     1801 atccggttgc tctggtaatc caccacgctt ccactcggcg cgggactgct gtcgccgaaa
     1861 tcgcgctgat cgagatcggt gaacttcgcc aaccggtcca caaagcgcag cttcacggta
     1921 tcggtcgcgc cgtttcggtg cttggcgacg atgatctccg ccacacccgt cgttgggttg
     1981 ttgtcttcgt cgtgcgtcaa gccgtagtac tccggacgat agatgaacat gaccatgtca
     2041 gcgtcctgtt cgatagcgcc cgattcgcgc aggtcgctga gctgcgggcg cttcgaccct
     2101 ccgcgggttt ccaccgcacg actcaactgc gagagcgcaa tgatcggtac gttgagttct
     2161 tttgcgatcg ccttgagtga acgcgaaatg gtgctgatct cctgttcccg gttgcctcct
     2221 ttgttctcca catcggcacg catcaactgc aggtaatcga tgatgatcat ctgcacatcg
     2281 tgcatcgctt tgagacgacg acatttcgaa cgaagatcga agaccgaaag cgcgggcgta
     2341 tcgtcaatga agatcggtga ttcggacagc cggccggtca tcgatacgag cttttgccac
     2401 tccgtttcgt ccagggtacc ctttcggagt ttatccgacg gaataccggt ctcggaagag
     2461 atcatccgct gcaccaactg gatggacgac atttccagcg agaagacggc gatcggtttg
     2521 ttgaactgga tggccgcgtt gcgtgcaaga gacaagacga aagccgtctt acccatggcc
     2581 gggcgggccg ccaggatgat cagatcggat ttctgccagc cggatgtcag tcggtccaga
     2641 tcggtaaagc cgctctcgac gccggtgata cccgtaccgt gctttttagc tgcttcgatc
     2701 agacgagttg cctcactgac catggcgctc atttcctgga aattccggcg gatgttgccc
     2761 tgggcgatgt cgaacaggtt cttttccgca cgatccagca agtggaatac gtccgtggta
     2821 tcctcgtagg catcccggat ggtctcggaa gaaatgcgga tcagttcacg ctgaatgtgc
     2881 ttctgcagga tgatccgcgc atggtattcg atgttggcgg cggaagcgac a
//
LOCUS       DAOTON010000305         2929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26499_length_2929_cov_3.484342, whole genome shotgun sequence.
ACCESSION   DAOTON010000305 DAOTON010000000
VERSION     DAOTON010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26499_length_2929_cov_3.484342"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="P5292_06820"
     CDS             <1..918
                     /locus_tag="P5292_06820"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRS38877.1"
                     /translation="SWGRANLHPGDEVLVSGMEHHSNIVPWQMISAERGATVRHIPIL
                     DNGELDLDAYHQLLGPKVRLVSVVHTSNSLGTVNPVKTMIDAAHAKNIPVLVDGAQAL
                     AHGTVDVSELDCDFLVFSGHKMLGPTGIGVLYGKEALLEAMPPYQGGGEMIHSVSFGG
                     TTYNELPYKFEAGTPNIADVIALGAALDYWASLDRTAVAEWEEQLLAYATATIVTIPG
                     VRIIGTAKRKSGVLSFIVEGLNALDIGMFLDTQGIAVRTGHHCTEPVMDRFGIPGTVR
                     ASFMFYNTAEEVDRFVEALRKAIHLLRKG"
     gene            922..1347
                     /locus_tag="P5292_06825"
     CDS             922..1347
                     /locus_tag="P5292_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRS38878.1"
                     /translation="MASIQELENAIVEDFALFDDWTEKYQYIIELGQQLPAMTDALKT
                     EDRKIKGCQSSVWLSASMQDGKVWFDADSDSTFVKGEIALLLRVLSGQAPEDVLKSEL
                     SFIDKIGLRQHLAMTRANGLAAMIKQIKLYALGFQSQQI"
     gene            1347..1700
                     /locus_tag="P5292_06830"
     CDS             1347..1700
                     /locus_tag="P5292_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF59 domain-containing protein"
                     /protein_id="HRS38879.1"
                     /translation="MDTPQTPAASPAPDATVKPLRDRIIDVLKSCYDPEIPVDIYELG
                     LIYEINISDGNDVHILMTLTSPACPVAETLPPDVEQKVRNIPDVNSAKVEITFNPPWD
                     KDMMSEEARMELGFW"
     gene            1966..2487
                     /locus_tag="P5292_06835"
     CDS             1966..2487
                     /locus_tag="P5292_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HRS38880.1"
                     /translation="MLPNKVAQSGLLTIDLEDWYDPTERVVFDLKEWLFEGLILREKD
                     FREGLKQHDFSRYQNKLVSLLCSADAIVPTWAFMLAASWLEPVARRVAFGTPDRLDEI
                     LYAEKIASLDPEVYRGQRVVIKGCSKVTVPVSAYTALTTFLRPIARSIHYGEPCSTVP
                     VYKRPKSQDDSHS"
     gene            2560..>2929
                     /locus_tag="P5292_06840"
     CDS             2560..>2929
                     /locus_tag="P5292_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRS38881.1"
                     /translation="MELTKDRYRAGGVDLLEVAAQFGTPLYVYDTDKIRSQYLSLVRS
                     FPDVDLKIKYACKANTNLSLLRFLRSIGAGLDAVSMEEVEFGLLAGFAPAEILFTPNG
                     VSFEEIRRGVQACVVVNIDNL"
BASE COUNT          714 a          742 c          803 g          670 t
ORIGIN      
        1 tcctggggac gtgccaacct gcacccggga gacgaagtgc tggtcagtgg catggagcac
       61 cacagcaata tcgtgccctg gcaaatgata agtgcggaac gcggcgctac ggtcaggcac
      121 attccgattc tggataacgg tgaactggat cttgacgctt atcatcaatt actcgggccg
      181 aaggttcgat tggtgtctgt cgtccatacc agtaactcgt tgggaaccgt caatccggtg
      241 aagaccatga tcgacgcggc tcatgcaaag aacatcccgg tactcgtcga cggcgctcag
      301 gcgcttgcgc atggaaccgt ggatgtaagc gagttggatt gtgatttcct cgtcttctcc
      361 ggacacaaga tgctgggtcc aaccggcatc ggggtgctct atggaaagga agcgttgctg
      421 gaagccatgc caccttatca gggcggaggc gagatgatcc attccgtttc gtttggcggc
      481 accacgtaca atgaacttcc ttacaagttc gaggccggca ccccgaacat cgcggatgtg
      541 attgccttag gagccgcgct ggattactgg gctagcctcg atcgaacggc tgtcgcggaa
      601 tgggaggagc aattgctcgc ctacgccacg gccaccatag taacgattcc cggagtacgc
      661 atcatcggaa cagcaaagcg caaatccgga gtcctgtcat tcatcgttga aggccttaat
      721 gcactggata ttggcatgtt cctcgacacc cagggtattg ccgtgcgtac cggtcatcat
      781 tgcaccgagc cggtgatgga ccggttcggt attcccggaa ccgtacgggc atcgttcatg
      841 ttctacaata cggcggagga agtcgaccga ttcgtcgaag cgctgcgcaa agcgatacac
      901 ttactccgga aaggctgagt catggcatcg attcaagaat tggaaaacgc gatcgtggag
      961 gatttcgccc tgttcgatga ctggacggag aagtatcagt acatcattga attggggcag
     1021 caattgcccg cgatgactga tgcgttgaag accgaagacc ggaagatcaa gggttgtcag
     1081 agcagcgttt ggttgtcggc ttcaatgcag gatggaaaag tatggttcga tgccgatagt
     1141 gacagcacct tcgtgaaagg ggagatcgct ttgttgttgc gtgtgctatc cggacaagca
     1201 ccggaagacg tattgaaatc cgaactcagt ttcattgata agatcggact acgtcagcac
     1261 cttgcgatga cccgggcgaa cggactggca gccatgatca aacaaattaa actctatgca
     1321 ctgggattcc agtcgcagca aatttgatgg acactcctca gacaccagcc gcttccccgg
     1381 cgccggatgc aacggtaaaa ccgctacgcg atcggatcat cgacgtgctc aagagctgct
     1441 atgacccgga gattcccgtg gacatttacg agctcggcct catctatgag atcaacatca
     1501 gcgatggaaa cgatgtccac attctcatga cactcacctc accggcctgt ccggtagcgg
     1561 agacgcttcc accggatgtc gagcaaaaag tccgcaacat accggatgtc aattccgcca
     1621 aagtagaaat caccttcaat ccgccctggg ataaggatat gatgagtgaa gaggcgagga
     1681 tggagttggg gttctggtga tgaggtagtg aatagtgaat agtgaacagt aaacagtaaa
     1741 cagtgaatag tgaatagttt agttttcaat taaacgagat tcaatcgcca ataccataca
     1801 acgattggca aatttttaat taccaataac caattactaa ttaccaatta ctaattacca
     1861 atcaccaatc accaatcact tccctcccca gacgcgcttc ctgaaaggta aactcaacta
     1921 acccggaact cgaaacacgg aacccggaac tactaacccc ctctcatgct tcccaacaaa
     1981 gtcgcccaaa gcggtctgct gaccatcgac ctggaggatt ggtatgatcc gacggaacga
     2041 gtcgtctttg atttgaaaga gtggttgttt gaagggctga tcctgcgtga aaaggatttt
     2101 cgggaaggct tgaagcagca tgatttttcc cggtatcaga ataaattggt ttcgctcctg
     2161 tgcagcgccg acgcgatcgt accgacctgg gcgttcatgc tggcggccag ctggctggag
     2221 cctgttgcca ggcgggtagc gttcggaacg ccggaccgat tggacgagat cctgtacgct
     2281 gaaaaaatcg cctcgcttga tccggaggtt taccggggcc agcgggtcgt catcaagggt
     2341 tgcagcaagg tgaccgtacc ggtttccgcc tatactgcct tgacgacctt tcttcgtccc
     2401 atcgcccgga gcatacatta cggcgaacct tgcagcacag taccggtgta caagcgcccg
     2461 aaatcgcagg atgattccca tagctgacgg ctaataactc gggcaggcgg gtttcggacc
     2521 gctacttcca acttttactt ttgggtaaac gatttaccca tggaactgac caaggaccga
     2581 taccgtgccg gtggagttga tttattggaa gtggccgcgc aattcgggac gcccttgtat
     2641 gtgtacgata cggacaagat ccggtcccag tatttatccc tggtacgatc ctttccggat
     2701 gtcgatctga agatcaagta tgcgtgcaag gcgaacacca acctgtcgct ccttcggttt
     2761 ttacgttcga tcggagccgg attggacgcg gtgtcgatgg aagaggtgga gttcgggttg
     2821 ctggcaggtt tcgcccctgc agaaatactt tttacgccca acggcgtttc attcgaggag
     2881 atccggcgtg gtgttcaggc ttgtgtggtg gtgaacattg acaatcttt
//
LOCUS       DAOTON010000306         2927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26522_length_2927_cov_3.826602, whole genome shotgun sequence.
ACCESSION   DAOTON010000306 DAOTON010000000
VERSION     DAOTON010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26522_length_2927_cov_3.826602"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="P5292_06845"
     CDS             complement(<1..269)
                     /locus_tag="P5292_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38882.1"
                     /translation="MTHTTRIKLLKNSCRIFFLILTALLPISSNGQECGSRTLDPEIA
                     GFLKIIGYQDLSLEQLRAMPIGQLKFPQIPLLPYPKEDVQRIKVTR"
     gene            complement(438..725)
                     /locus_tag="P5292_06850"
     CDS             complement(438..725)
                     /locus_tag="P5292_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HRS38883.1"
                     /translation="MRYEFTAPLWQYAGAGGWHFVSLPKKMSKEIRKLLRSEEQGWGR
                     LPATARIGQSEWKTAIWFDTKQDTYLLPVKGEVRKSEGLVAGNKISTSLWI"
     gene            complement(808..1344)
                     /locus_tag="P5292_06855"
     CDS             complement(808..1344)
                     /locus_tag="P5292_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020605891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS38884.1"
                     /translation="MNTYPKVIVRSAMLPDDLEAVRKLWMDYLSWGNNKMQELYGVHP
                     HHPAETVEQDIRQISKFQPPHGQLLLAVHEGRVCGLGSLKSISADVGEIKRMFVDPGL
                     RRIGAGRAILEGLLTEAREIGYKTVRLDSPRFMEAAHALYRSVGFRDIDAYPEVEIPE
                     AFKAYLLFMELDLTKEPQ"
     gene            complement(1484..2032)
                     /locus_tag="P5292_06860"
     CDS             complement(1484..2032)
                     /locus_tag="P5292_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRS38885.1"
                     /translation="MRKVVAAFNMTLDGNCDHTAGLPDEEVHQHYTDLLDRSDVILYG
                     RTTYQLMEYWRTFLEKPSGERTMDTFAAAIDRIPKIVFSRSLKDVDWKSAGLAELELK
                     ETVERLRQQPGRDILIGSRSLIQQLMKLRLIDELQLCIHPVVAGKGMSLFEDMDPTVL
                     RLVNAKRFDGGAIVLYYKLEAS"
     gene            complement(2165..2605)
                     /locus_tag="P5292_06865"
     CDS             complement(2165..2605)
                     /locus_tag="P5292_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRS38886.1"
                     /translation="MKATRPIHPDFQHFLKFKDKEVIDLFTDLREYILELYPDSNELL
                     YHTHALTAVFSISEKLSDAFCMLPIYTKHLNLGFNKGTLLKDPHQLLTGTGNLIRHID
                     VKKPGDYRNPKVKALIKAAIDFAIKDMEKPTKSVGKTISKIAKK"
     gene            2674..>2927
                     /locus_tag="P5292_06870"
     CDS             2674..>2927
                     /locus_tag="P5292_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38887.1"
                     /translation="MNVPNSLGKNGKHCFQIDAQTPFDQLAGSDRNRFQFGDLLFEFK
                     GTLDDETALLFPHFGLIPKIRISHHSRRFPYLRPLMGRRYS"
BASE COUNT          668 a          777 c          693 g          789 t
ORIGIN      
        1 cgggtcactt tgatccgttg tacatcttct ttcggatagg gcaacaaggg gatctgcggg
       61 aatttcaatt gcccgatcgg catcgcgcgt aactgctcca agcttaggtc ctgataaccg
      121 atgatcttta aaaatccggc tatctcggga tccagtgtcc gggaaccgca ttcctggccg
      181 ttgctgctga tgggcagcag ggctgtcagg atcaggaaga atatacgaca gctattctta
      241 agcaacttga ttcgcgttgt gtgtgtcatg gagccgagtt aggaaatcag aaaaccgcag
      301 gatgtgcgat cggtgtatat agcgtgcttc taagattgat gcaacaaatg tattcaacgg
      361 gcatgcggca ggataggaca gtactaaaaa cgaataggac ttcggtcaac atctaccgaa
      421 catcggtgca atcctgctca aatccacaga ctggtgctga tcttgttgcc agcaaccagc
      481 ccttccgact tccgaacctc tcccttcacc ggcagcaaat aggtatcctg ctttgtatcg
      541 aaccagatcg ccgtcttcca ttcgctttgt ccgatccgtg cggtagccgg caaccggccc
      601 catccctgct cttccgatcg taacagcttg cggatctcct tcgacatctt cttcgggagc
      661 gacacgaaat gccagccgcc ggctccggca tactgccaaa gcggggcggt gaattcatac
      721 cggatgcctt tcccgaacac ttcagcaagg tagaaaagaa gttgttgttg aatctggaaa
      781 aacgccatcc aacggaccgg tcattcttta ctgcggctct ttggttaagt ccaactccat
      841 aaataacaga taggctttaa aggcctccgg aatttccact tccggatagg catcaatatc
      901 acggaagcca acacttctat acagtgcatg cgcagcttcc atgaaccggg gactgtcgag
      961 ccggaccgtt ttgtatccga tctctcttgc ctctgtcaag agtccttcca gtatggctct
     1021 gccggcccct attcgtctga ggcccggatc cacgaacatt cgtttgattt caccgacatc
     1081 cgcgctgatg ctcttcaagc ttccaagtcc acaaaccctg ccttcatgta ctgccaacag
     1141 cagctgaccg tgcggtggct ggaacttact gatctgacgt atatcttgtt ccacggtctc
     1201 cgcaggatga tgcggatgaa ctccatagag ttcctgcatt ttgttgttac cccaactcaa
     1261 gtagtccatc cacaattttc tgacggcttc caagtcgtcc ggaagcatcg cgcttcgtac
     1321 aatcaccttc ggatatgtgt tcattggatg tagttcttgt ccgattgttc aaattgtgaa
     1381 tctatgcaat gggcgtaatg agcactctaa accggtttca ttttatacgt ttcgattagc
     1441 gcatgacctt tcctaccgga accagtctaa gctccattac acatcacgat gcttccagtt
     1501 tgtagtatag cacgatagca ccaccgtcaa agcgctttgc attgaccagc cggagaacag
     1561 tcgggtccat gtcctcaaac aaggacattc cctttccggc aacgaccggg tgaatgcaca
     1621 actgcaattc gtctatcagc cgcagcttca tcaattgttg gatcagactt cgacttccga
     1681 tgaggatatc ccttcccggt tgctgccgga gacgctcgac ggtttctttc agttcaagtt
     1741 cagccaggcc ggcgctcttc caatccacat ccttcaagct ccgggagaac acgatcttgg
     1801 gaatccggtc gatcgctgcc gcgaacgtat ccattgtccg ctcgccggaa ggtttttcca
     1861 aaaatgtccg ccagtattcc atcaactgat aggtcgttcg gccgtagagg atgacatcgc
     1921 tgcggtccaa cagatccgta taatgctgat gtacctcctc atccggcagt cccgccgtat
     1981 ggtcgcagtt cccatcgagg gtcatgttga aggcagcgac aacttttctc atatgtcaac
     2041 ttggttttcg agttgctttg gttagatttc atgatgcgca gtagggtagg ctataacgcc
     2101 tgcgcggctt acaggctgca agacccggga tcgtatcatg aatcacccgc aatgcgaccc
     2161 gttttcactt cttcgcaatc ttggaaatcg tcttgccaac tgatttcgtc ggcttttcca
     2221 tatccttgat ggcaaagtcg atggccgctt tgatcagggc tttcactttc ggattccggt
     2281 aatcacctgg tttcttcaca tcgatgtgcc ggatcagatt tccggtgccc gtcaggagct
     2341 gatgcggatc cttcaacagc gtccccttgt tgaaacccag gttcaggtgc ttggtataga
     2401 tcggcaacat gcaaaaggcg tccgacagct tttccgaaat ggagaaaacc gctgtcaggg
     2461 cgtgtgtatg atacaggagt tcattcgagt ccggatacag ttccaggatg tactcccgaa
     2521 gatcggtaaa caagtcgatc acttccttgt ctttgaactt gaggaaatgc tggaagtccg
     2581 ggtgtatcgg tcgtgtggct ttcatcttat taattggtca gcttatatgc ccgcttactt
     2641 attcaggtct gttattggtc gaaggggaat tcaatgaatg ttccgaactc gttgggcaaa
     2701 aatggcaaac actgtttcca aattgatgcc caaactccgt tcgaccaact tgctggtagt
     2761 gatcgcaacc ggtttcaatt cggtgatctg ttgtttgaat ttaaaggtac gttggatgac
     2821 gagactgctc tcctgtttcc ccactttgga ttaataccca aaataagaat ttcacaccat
     2881 agtcggcgtt ttccttacct caggccgttg atgggccgcc ggtattc
//
LOCUS       DAOTON010000307         2927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26527_length_2927_cov_2.481894, whole genome shotgun sequence.
ACCESSION   DAOTON010000307 DAOTON010000000
VERSION     DAOTON010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26527_length_2927_cov_2.481894"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1005)
                     /locus_tag="P5292_06875"
     CDS             complement(19..1005)
                     /locus_tag="P5292_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRS38888.1"
                     /translation="MMDIKEINERIQRESAFVDLLQLELGKVIVGQKQMTERLLIALL
                     ANGHILLEGVPGLAKTLSIKSLASAIHAKFNRIQFTPDLLPADLIGTMIYNQKQESFS
                     IRRGPVFANFVLADEINRAPAKVQSALLEAMQERQVTIGEQTFALEEPFLVLATQNPI
                     EQEGTYPLPEAQVDRFMLKVVIGYPTKEDEKLIIRQNIGQAPAPVQPVVRPEEILQAR
                     KSLRDVYMDEKIERYIVDIVFASRTPEDYKLGKLKQLISYGGSPRASINLALASKAYA
                     FVKRRGYVIPEDVRAICHDVMRHRIGLTYEAEAENISSDQIINEILNAVEVP"
     gene            complement(1163..2383)
                     /locus_tag="P5292_06880"
     CDS             complement(1163..2383)
                     /locus_tag="P5292_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS38889.1"
                     /translation="MSIRLSEFTVFKGSKHQRRAIRMLGVFILVALLAPVLANRNPLM
                     LRIDGKTSFPALSGNPYVELPQENGEWKAVLAADIDWRTFQRGFTLLAPIPYAPADLD
                     PANANFQSPFSKQFLASDTGSIELPLRYRHWLGTTRTGGDLLSALIHGTRYALLIGFL
                     AALLSALIGITLGLFAGYFGDRHFLVHPGSFILILALFFIAWFEGSFIPGRYGLQASL
                     GWWLLPLIGMLLTLRVRGKKATLRLPIDTMVRAVMSVFLAVPRLVWIILIGATLTPSV
                     SLVILTIGLTGWAEFTQLTRAETLRLRGSGFEENARAAGLRDVRILFRHLLPNFGSLL
                     VLIFFYHMAGAILAESALSFLGAGVPHDIVTWGSLLQEGRQDLQAWWVLVFPGCCLFL
                     LLNSLQSMRRTTGS"
     gene            complement(2380..>2927)
                     /locus_tag="P5292_06885"
     CDS             complement(2380..>2927)
                     /locus_tag="P5292_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS38890.1"
                     /translation="VLLRANPYIGQIVPVTGVSPAGGWPASASWMSRLYGTIPYLILP
                     TVSYCYGSWAFLTGQFRETLFREMQSDYFKTALAKGLDQKKALYVHAFRNTWIQAFAL
                     FGFAFPAAIGGSIVIESIFGIPGMGFTIYEAIDARDFPVMNAVFLLSGLFTLIGLLIS
                     DLLVYRSDPRIHSPSTSSTVS"
BASE COUNT          692 a          764 c          852 g          619 t
ORIGIN      
        1 ttcgctctcg ggggagactt agggaacttc gactgcgttg aggatctcgt tgatgatctg
       61 gtcggaactg atgttctcgg cttccgcttc gtaggtcagc ccgatgcggt gccgcatcac
      121 gtcgtggcag atcgcgcgta cgtcttcggg aatcacatag ccgcggcgtt tcacgaaggc
      181 gtaagccttg gacgccagcg ccaggttgat gctcgcgcgg ggcgatcctc cgtagctgat
      241 cagttgtttc agcttgccga gtttgtaatc ctcgggtgta cggctcgcga agacgatgtc
      301 gacgatgtaa cgctcgatct tttcgtccat gtacacgtcg cgcaggcttt tgcgcgcctg
      361 caggatctct tcgggtcgca ccaccggctg caccggagcc ggcgcttgcc cgatgttctg
      421 acggatgatg agtttttcat cttccttcgt cgggtagccg atcaccactt tcagcatgaa
      481 gcggtcgacc tgtgcttccg gaagcggata ggttccttcc tgttcaatgg gattctgcgt
      541 agccaggacc aggaaaggtt cttcgagcgc gaaggtttgt tcgccgatgg taacctgtcg
      601 ttcctgcatg gcttccagca aggcgctttg caccttggcg ggtgcccggt tgatctcgtc
      661 ggccagcacg aagttggcga agacaggtcc ccggcggatg gagaaactct cctgcttctg
      721 attgtagatc atcgtgccga tcaggtcggc aggtaacagg tccggtgtga attggatccg
      781 gttgaacttg gcgtgaatgg ctgatgcaag cgacttgatc gaaagcgtct tggccagtcc
      841 cgggactcct tccagcagga tgtggccgtt ggccagtagg gcgatcagga gccgttcggt
      901 catttgcttt tgtccgacga tcactttccc caattcgagt tggagcaagt caacaaaagc
      961 agattcgcgc tgaatgcgct cgttgatttc tttaatgtcc atcatgttgt ttaactacga
     1021 ggtactggta cctaaaccgc gaaaccgtgg tttagaaagt gtaacattcg gaaaggatgg
     1081 cggtagccgg aaaccttcgt aacgaggagc aattttaact aaaaaacgat gaatccgctg
     1141 tgaaaaacga ggttaaagaa tgttaagaac cggttgttct tcgcatggac tgaaggctgt
     1201 tcaagagcag gaacaggcag caacccggaa ataccagcac ccaccacgcc tgcaaatcct
     1261 gtcgaccttc ctgcaacagg cttccccagg ttacgatatc gtgcggaaca ccggcaccga
     1321 gaaacgacaa agcggactcg gccaggatcg ctccggccat gtgataaaaa aagatcagga
     1381 ccaacaggga tccgaaattc ggtaacaaat gccggaagag gatacgtaca tcccgcaaac
     1441 ccgcggcgcg cgcgttttcc tcaaacccgg atccgcggag tcgcagggtt tccgctctgg
     1501 tcagttgggt gaattccgcc catccggtca agccaatggt caggatcacc agcgagaccg
     1561 atggtgtcag cgttgcaccg atcaggatga tccagactag ccggggtact gccaggaaca
     1621 cgctcatgac cgcacggacc attgtatcaa tcggcagtcg cagggtggct ttcttcccgc
     1681 gaacgcgcaa ggtcagtaac attccgatca atggtaataa ccaccaacca aggcttgctt
     1741 gaagtccgta acggccagga atgaaactgc cttcgaacca ggcgataaag aaaagtgcca
     1801 gtattaagat gaacgagccc ggatgcacca ggaagtgacg atcaccaaag tatccggcga
     1861 ataaccccag tgtaattcca atcagggcag agaggagcgc tgccagaaag ccgatcagca
     1921 aggcataccg ggtgccatgg atgagggcgg atagcaggtc tccacctgtc cgcgttgtgc
     1981 ccagccagtg acggtagcgc aatggaagtt cgatcgagcc ggtgtcggat gccagaaact
     2041 gtttcgagaa gggtgattga aagttcgcgt tggcgggatc caggtcggca ggagcatacg
     2101 ggatcggagc aaggagggtg aagcctcgtt gaaacgtgcg ccagtcgata tcggcggcta
     2161 gtactgcttt ccattctccg ttttcctgcg gtaactccac atacggattt ccgctcagtg
     2221 ccgggaagct ggtcttgccg tcaatacgaa gcatcagagg attacggttc gccagaacag
     2281 gtgccagcag cgccaccagg atgaaaacac caagcatgcg aatcgcccgc cgttgatgtt
     2341 tcgagccttt gaacacggta aactccgaca accgaatgct catgataccg tgcttgaagt
     2401 tgaaggcgaa tgaatccggg ggtcggaacg atacacgagc aggtcggaga tcaggagccc
     2461 gatcagcgta aacaatccgc tcagcagaaa gaccgcattc atgaccggaa agtcgcgtgc
     2521 atcgatcgct tcgtagatcg tgaatcccat ccccggaatt ccgaagatgg actcaatgac
     2581 gatggaacct ccgattgctg ccgggaaggc gaagccaaaa agtgcgaagg cctggatcca
     2641 ggtgttgcga aacgcgtgga cgtagagggc ttttttctga tcgagtcctt ttgcaagcgc
     2701 ggtcttgaaa taatcggact gcatttctcg gaaaagtgtc tctcggaatt gaccggtgag
     2761 aaatgcccaa ctgccatagc aataggatac agtcggcagg atgaggtagg gaatcgtgcc
     2821 gtacaatcgt gacatccagg aagcggatgc cggccatccg ccggcaggcg agaccccggt
     2881 gaccggcacg atctgcccga tgtaaggatt tgcccgcaac agcactt
//
LOCUS       DAOTON010000308         2906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26759_length_2906_cov_3.733427, whole genome shotgun sequence.
ACCESSION   DAOTON010000308 DAOTON010000000
VERSION     DAOTON010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26759_length_2906_cov_3.733427"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="P5292_06890"
     CDS             <1..915
                     /locus_tag="P5292_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38891.1"
                     /translation="GWRLVTPRGRSVTARFVKPGRVVRRWDFDHFLFEQLRVKFPTLR
                     LLTGLTVKSIRNTGEGFELLADGLQLKARFLVGCDGANSIVARQLVGRTVDPAFHSGA
                     VRAYYRGIKGVQGNELLEIYLLKDFLPGYFWIFPLPDGAANVGFGMLTSDISKRRIDL
                     KAALDAIIRSNDELRDRFRFAVREGTVTGFGLPLGGKVQQLSGDSFLLCGDAASLIDP
                     LNGEGIANAMWSAQIAARHIRQAFKSGAFGHDSLQAYDRELYSKLGPELRRKLLMQRV
                     FNRPWLIESLVALGNRIPWLKDRVARRL"
     gene            996..2078
                     /gene="aroC"
                     /locus_tag="P5292_06895"
     CDS             996..2078
                     /gene="aroC"
                     /locus_tag="P5292_06895"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRS38892.1"
                     /translation="MAGNSFGQIFRLTSFGESHGPALGGVLDGCPSGLPIDLTAIQAD
                     LDRRRPGQSGITTARKEADSFELLSGVFEGRTTGAPIGFIIRNRDERSSDYDAMAGSF
                     RPSHADYTTTAKYGHRDHRGGGRSSARETVCRVFAGAIARQLLTTVGVEVGACVSGVG
                     PYQFATADRFFSREEVDANILRCPDETAADQMIRYIEELKSLGDTTGGVITGFVRGMP
                     AGWGEPVFDKLHADLGKAMLSINAVHGFEYGSGFAAAAMKGSEHNDVFTTAKAGRIVT
                     RTNHSGGIQGGISNGMDIRFRVAFKPVATLLKPQETVDTGGNPTVLEAKGRHDPCVVP
                     CAVPIVEAMTCLVLADHYLRHRSSRL"
     gene            2124..>2906
                     /locus_tag="P5292_06900"
     CDS             2124..>2906
                     /locus_tag="P5292_06900"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRS38893.1"
                     /translation="MKKILKWFFIVIGVLLVLLIATPFLFKDKLVGLIKEETNKNLNA
                     TVDFSDASLSLIRSFPNLSLELENLSVINMAPFAGDTLVYAKSLGITVDIMSVIRGSE
                     INIRSVTVADAVMNFLVTKEGKANWDIAKPSETATAAGEPSKFKASLKSYTVSNADVT
                     YDDRSLDFYLRLEDVNHAGKGDFTQDLFVLSTTTISPNVAMKYGGVPYIAAAKAEILA
                     DLDMDMKQFKFTFKQNQLKLNELELGVDGWVAMPDTNIDMDLK"
BASE COUNT          651 a          754 c          804 g          697 t
ORIGIN      
        1 ggctggcgat tggtgacacc ccggggcagg tccgttacag ccaggtttgt aaaacctggc
       61 cgggtcgttc gccgatggga cttcgatcat tttctgttcg aacaactccg ggtgaagttt
      121 ccgacactca ggttgttgac cggattgacg gtcaagtcga tccggaatac cggagagggg
      181 tttgaattgt tagctgacgg gctgcaactc aaggcccgtt ttctggtggg ctgtgatggc
      241 gcaaactcca tcgttgcccg gcaactggtc gggagaacgg tagatcctgc ttttcactcg
      301 ggagcagtcc gggcttatta ccgcgggata aaaggtgttc aaggcaatga gttgctcgag
      361 atctacctgc tcaaagattt tctgccggga tacttctgga tatttccgct tccggatgga
      421 gcagcgaatg tgggtttcgg gatgttgacc agcgatatct ccaaacggcg gatcgatctg
      481 aaggctgccc tggacgcgat tatccggtcc aatgatgaac tgcgtgaccg attccggttc
      541 gccgtaaggg aaggaacggt gacaggattc ggcttaccac ttggcggaaa ggtgcaacaa
      601 ctcagcggtg acagtttcct actctgtggc gatgccgctt ccctgatcga tcctttgaac
      661 ggggaaggta tagccaacgc gatgtggagt gcgcaaatcg cggcacgcca tatccggcag
      721 gccttcaagt cgggagcgtt tggccacgac tctttgcaag cctacgatcg ggaactctac
      781 tccaaacttg gaccggaact ccgccgcaaa ttgttgatgc aacgtgtttt taaccgtccc
      841 tggctgatcg agtccctggt tgcgttgggg aaccgaattc cctggttgaa agaccgggta
      901 gcacgcaggt tgtaaggctg cattaacatc ttttcgttga tttttaggcc tttcaccggg
      961 ttctgcagcc aacccgaacc gtaacttcgt atcctatggc tggaaatagc ttcggacaga
     1021 tttttcgtct tacctcgttc ggcgaatcgc acggtcccgc actgggtggc gtactcgatg
     1081 gttgcccgtc cggactgccg attgatttga ctgctataca agctgatctt gatcgacgga
     1141 gacccggaca aagcggcatt accaccgcga gaaaggaggc tgattcattc gagttgttat
     1201 cgggtgtgtt tgagggcaga accaccggcg ctccgatcgg atttatcatc aggaaccgtg
     1261 acgaacgttc ttccgattac gatgccatgg ccggaagctt tcgtccttct cacgccgatt
     1321 atacgactac cgccaaatac ggacatcgcg atcatcgagg tggtggacgc agttccgccc
     1381 gcgaaacggt ttgccgggtc tttgcaggcg cgatcgcccg tcaactgttg actaccgtcg
     1441 gtgtggaagt aggcgcttgt gtttcgggtg tcggtcccta tcagttcgcc actgccgatc
     1501 gctttttcag ccgcgaggaa gttgacgcga acatactccg ttgtccggat gaaaccgccg
     1561 ctgatcagat gatccggtat atcgaagagc taaagtcact gggcgatacc accggcggtg
     1621 tcatcaccgg cttcgtccgt ggcatgccag ccggatgggg tgaaccggta ttcgataaac
     1681 tccacgccga tctgggtaag gcgatgttga gcatcaacgc cgttcatgga ttcgaatacg
     1741 gaagcggctt cgctgccgcg gccatgaaag gctctgaaca taacgatgtc tttacgactg
     1801 cgaaggctgg ccggatcgtg acgcgtacca accattccgg gggaatccaa gggggtattt
     1861 ccaatgggat ggacatccgt ttccgcgtag ccttcaaacc cgtcgccacc ctgctgaagc
     1921 cgcaggaaac agtcgatacg ggaggaaatc ctactgtcct ggaagccaaa gggcggcacg
     1981 atccgtgcgt agtgccgtgc gccgttccga tcgtcgaagc gatgacctgt ctggtgctgg
     2041 ccgatcacta tctgcgccac cgctcctccc gcttgtaaac aatatttatc gaaaatcccg
     2101 ttagtataat atcaattaaa gtcatgaaaa aaatactgaa gtggttcttc atcgtaatcg
     2161 gagtcttgtt ggtgctattg atcgccactc cgtttctttt caaggacaaa ttggttggcc
     2221 tgatcaaaga ggaaacgaat aagaacctga acgcgacggt tgacttctcg gatgccagtc
     2281 tttctttgat ccggagtttc cccaacctct ccctggagtt ggagaatcta tcggtcatca
     2341 acatggcccc cttcgccggt gataccttgg tctatgcgaa gtcactcggc attacggtcg
     2401 atatcatgtc ggtcatccga ggtagtgaga tcaacatccg ctccgtcacg gtcgcggatg
     2461 cggtcatgaa tttcctggtc accaaagaag ggaaagcgaa ctgggatatt gccaaacctt
     2521 ctgaaacggc cacggctgca ggtgaaccat cgaaattcaa ggctagcctg aagtcgtata
     2581 cggtttccaa cgccgatgtc acctacgatg accggtcgct cgatttttac ctgcgccttg
     2641 aagatgtgaa tcatgcaggc aaaggagatt ttacccaaga cctgttcgtc ctgtcgacca
     2701 ctaccatcag tccgaacgtt gcgatgaagt atggtggtgt accgtatatc gctgcggcaa
     2761 aagccgaaat tctggccgac ctcgacatgg acatgaagca attcaagttc acgttcaagc
     2821 aaaatcaact caagctcaat gaacttgaac taggtgtcga tggttgggtg gccatgccgg
     2881 acaccaacat cgacatggac ttgaaa
//
LOCUS       DAOTON010000309         2901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26827_length_2901_cov_2.566058, whole genome shotgun sequence.
ACCESSION   DAOTON010000309 DAOTON010000000
VERSION     DAOTON010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26827_length_2901_cov_2.566058"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="P5292_06905"
     CDS             <1..602
                     /locus_tag="P5292_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38894.1"
                     /translation="GGGKKPYHVFFFLNAFSLLIMLQFSTWLFLNGMGRRYFIGVYVS
                     LVIALLLWLQEMSEVKPRFLPAVARSLIGTALIGVFTTLYHFARHDPGNLTSVRSRIS
                     ALPKDIGIIGNYWNSYIFSIQHPGRVIATPHERDVVRNPLLVQEVFRQPRLFLVRDQW
                     MESFPDSIWQFGRRLERIGKERFMAGSYFCEYRIAVANE"
     gene            612..1898
                     /gene="purD"
                     /locus_tag="P5292_06910"
     CDS             612..1898
                     /gene="purD"
                     /locus_tag="P5292_06910"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRS38895.1"
                     /translation="MNILLLGSGGREHALAWKLAQSPRCSRLFVAPGNAGTALVGTNV
                     SMSVTNFDAIGRFVLEQQVGLVVVGPEEPLVKGIRDFFEADANLKQVPLIGPSQLGAQ
                     LEGSKDFAKAFLTRHHIPTARYKTFTSDNLEEGYTFIDSLPAPYVLKADGLAAGKGVV
                     ILEDAEAAKAELKEMIVGKKFGAASTQVVIEEFLRGIELSVFVLTDGRSYRLLPEAKD
                     YKRIGDRDTGPNTGGMGAVSPVPFATPEFLKKVEERIVVPTVQGLAHDGIAYNGFIFI
                     GLMNVGGDPYVIEYNVRMGDPETEAVIPRITGDLVELLLATGQGRLSEVPLAIDPRTA
                     VTVVLVSGGYPNDYKKGIQVSGHSEGGESLVFHAGTSGNERGEVVTNGGRVFAITSFG
                     RDIASARALSYAMADKIRYEGKYHRTDIGLDLLGEH"
     gene            complement(1999..2181)
                     /locus_tag="P5292_06915"
     CDS             complement(1999..2181)
                     /locus_tag="P5292_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38896.1"
                     /translation="MVAFWTAFWNFIGSFFTGVFTFVPPIGTFFNKFLIAIGFIAFFT
                     WMWYQNKYKSAPEQMD"
     gene            2257..>2901
                     /locus_tag="P5292_06920"
     CDS             2257..>2901
                     /locus_tag="P5292_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38897.1"
                     /translation="MIRFFRSPQPAALIVVPVIVLILWGQRFTDLPLAPAEAGGPLWG
                     LLVSMLGGLPGFLRFLLAVGTLSLSAIYFNLMVNRHEVLYKNSYLPSLFYALLASASV
                     ELLQFHPLHLVNLVLLRVLDKTFELYKSERPVRGLFDGGFLSGLCGLLYPPAGLLVLL
                     ILLGILLLRPFNFRELLIHLVGLVLPYFFFSVLAFWNGHLLDSWIRLWKPFRDPA"
BASE COUNT          568 a          769 c          871 g          693 t
ORIGIN      
        1 gggggggggg taaaaaacct tatcatgttt tctttttcct gaatgctttt tcactactga
       61 tcatgttgca attctcgacc tggttatttc ttaatggtat gggtcgtaga tattttattg
      121 gagtatatgt ctcgctggtt attgcattgc tgctttggtt gcaggagatg tcagaagtaa
      181 aaccacgctt tctgcctgct gtggcacggt cgttaatagg gacagctttg attggtgtat
      241 tcacgaccct ttaccatttc gccaggcatg accccggaaa tctgacctcg gttcgaagtc
      301 gtatctccgc tttgccaaag gatattggaa ttatcggcaa ctactggaat tcctacatat
      361 tctccattca acatcccggt cgggtcattg caacgcctca tgaacgggat gtggtgcgga
      421 accctttgct ggtgcaggaa gtcttcaggc aaccgcgtct atttctggtg cgggatcaat
      481 ggatggaatc ctttccggat tcgatttggc agttcgggag aaggctggaa cgaataggga
      541 aggagcgatt catggcgggt tcctatttct gtgagtaccg aattgcggta gcgaatgagt
      601 aatttcgccc catgaacatc cttcttcttg gatccggtgg ccgggagcat gcgctggcct
      661 ggaagctggc gcaaagtccg cgctgcagcc gcttgtttgt cgccccgggc aatgccggta
      721 cggccctggt cggtaccaat gtgtcgatgt cggtaacgaa tttcgacgcg atcggtcgat
      781 ttgtactcga gcagcaggtg ggcctggtcg tcgttggtcc ggaagaaccc ctggtgaaag
      841 gcattcgcga ctttttcgaa gccgatgcaa acctgaagca agtgcctttg atcggcccct
      901 cgcagcttgg cgcgcaactg gaaggcagca aggatttcgc caaggcgttt ctcacccggc
      961 atcacatccc cacggcccgc tacaagacct ttacatccga caacctggag gagggttata
     1021 cattcatcga ctccctgccg gcgccgtatg tgctcaaggc cgacggcctc gcggcgggca
     1081 aaggcgtggt gatcctcgag gacgcggaag ccgccaaggc ggagttgaag gagatgatcg
     1141 taggaaagaa gttcggagca gccagcacgc aagtggtcat cgaggagttc ctgcggggga
     1201 tcgagttgtc ggtcttcgtg ctgaccgacg gtcgctcgta ccgcctgttg cccgaagcga
     1261 aggactataa gcgcatcggc gaccgggaca cgggtccgaa caccggaggt atgggcgcgg
     1321 tcagcccggt gccgttcgcg acgccggagt ttttgaagaa agtggaagag cgtatcgtgg
     1381 tgccgaccgt tcaaggactg gcgcacgacg gcattgctta caacggtttc atcttcatcg
     1441 gactgatgaa cgtcggcggc gatccgtacg tcatcgagta caacgtccgc atgggcgatc
     1501 cggagaccga ggcggtcatc ccgcgcatca ccggcgacct ggtggagttg ctgctggcga
     1561 ccgggcaggg acggttaagt gaagtgccgc ttgccatcga tccccgcacg gcggtgaccg
     1621 tggtactggt cagcggcggt tatccgaacg attataagaa aggcattcag gtgtccggac
     1681 atagtgaagg cggggagagc ctcgtgtttc acgcgggcac ttccggcaac gagcggggag
     1741 aggtcgtcac caacggcgga cgcgtcttcg cgatcacctc gttcggccgc gacatcgcgt
     1801 cggcgcgcgc cttgtcatat gcgatggcgg acaagatccg gtatgaaggg aagtatcacc
     1861 gcaccgacat cggactggac ctgcttggag agcactgagc gtgggttgcc cgcgcggcgt
     1921 cctgcgttca catgcaccca cgaaaaaagc cactcgcaga tcgcgaatgg ctttttcagt
     1981 ttatcggaaa cgacggaatc aatccatctg ttcaggcgcg gatttgtact tgttctggta
     2041 ccacatccag gtgaagaagg cgatgaagcc gatggcgatc aggaacttgt tgaagaaggt
     2101 tccgatcgga ggaacgaacg tgaatacgcc ggtgaaaaaa cttccgatga agttccagaa
     2161 ggcggtccag aatgctacca tgttgctttt gtttacgggg caaatataga aaggattccg
     2221 ggcatccgca agccgaaaaa agctactttt agagcgatga tccgcttttt ccgatccccg
     2281 cagcccgccg cgctgatcgt cgtcccggtc atcgtattga tcctgtgggg ccaacggttt
     2341 accgacctgc ccctggctcc cgccgaagcc ggcggacccc tttggggctt gttagtcagt
     2401 atgttaggcg gattgccggg tttcctgcgc tttctgttgg cggtcgggac gctgtccctg
     2461 tcggccattt acttcaacct catggtcaac cgccacgagg tcctttacaa gaattcatac
     2521 ctgccctcgc tgttctatgc cttgctcgca tcggcttccg tggaactctt gcagtttcac
     2581 ccgctgcacc tggtgaacct ggtgctgttg cgggtgcttg acaagacgtt cgaattgtac
     2641 aagagcgaac gcccggtgcg cggactcttc gacggcggat tcctcagcgg actctgcggg
     2701 ttgttgtatc cgccggccgg tttgctggtg ctgctgatcc tgctcggcat cttgttgttg
     2761 cgtccgttca acttccgcga gttgttgatc cacttggtcg gactcgtgct gccgtacttc
     2821 ttcttttccg tactggcttt ctggaacggg catttgctgg attcgtggat caggctctgg
     2881 aagccattcc gcgatccggc c
//
LOCUS       DAOTON010000310         2892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26921_length_2892_cov_3.601692, whole genome shotgun sequence.
ACCESSION   DAOTON010000310 DAOTON010000000
VERSION     DAOTON010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26921_length_2892_cov_3.601692"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1795
                     /locus_tag="P5292_06925"
     CDS             <1..1795
                     /locus_tag="P5292_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS38898.1"
                     /translation="GDVTGARAAYTAALGRARDLGDQPTVALLNALIVRTYENETGSH
                     AKALPFAREAFLSTPANAACALATRARHYGELLLALERDAEALPVLKKSLEHVNTCGD
                     RLQKAEILRDLGILSYRSGRYEEALRYFEKSQQADPKLPALRLMRDSYLKLYAGSRIA
                     QDSSRSQYYSRLYRLFKDSVEQLLNSRMLSPDSFTRELAEKSRVLELIERPTALEGRA
                     LSTDQLAYNQQLTEAELERLKTEEALARMNQEKMQDKVADNEREEKIRELERQQALQE
                     LELSKKALRESRQRQLINLLLAGVVLIIAFLGFLAYRFRVNKKAHLKLDRAYSELKKT
                     HQQLKATQEQLVHSEKMASLGQMTAGIAHEIQNPLNFVNNFAESSAELLEELRQAGSK
                     EEQEELLSDLQINLGKIVHHGKRADGIVKSMLQHSRGGDPVKSMVQLNKLAEEYLHLA
                     YHGMRAKDPGFNCRLEKQLDPALPEVPAVPQEIGRVLLNLLSNAFYAVGERTERLRSS
                     DEVYHPEVRVSTVSLSRYVALSVGDNGTGIPATVRERIFEPFFTTKATGQGTGLGLSI
                     SYDIVKAHGGEMKVETEEGRGTIFTLLLPIA"
     gene            complement(1848..2243)
                     /locus_tag="P5292_06930"
     CDS             complement(1848..2243)
                     /locus_tag="P5292_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HRS38899.1"
                     /translation="MRVDLYSQNIIKSKTMATKKAKKKAPAKKAKKAVAKAKPKAKAK
                     AKTKRKPNAAFMRPLAIGEALQPVVGSKPLPRTEVVKKLWAYIKKNKLQDAKQKRNIN
                     ADEALKKVFGGKKTVSMFEMTKLVSKHLK"
     gene            2558..>2892
                     /locus_tag="P5292_06935"
     CDS             2558..>2892
                     /locus_tag="P5292_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38900.1"
                     /translation="MKHFSLLFFILASLFLPTHTRAQQDSLLNTSRVGCLSGDCEKGI
                     GTYRNSAGSVYTGPFSEGKFEGKGKMIFRDGDVYEGDFQRGKMTGSGTYTFKSGTSYT
                     GQFTDGKMDG"
BASE COUNT          660 a          834 c          797 g          601 t
ORIGIN      
        1 cggcgatgtt acgggcgcgc gagctgctta tacggccgca ttggggcgcg cgcgcgatct
       61 cggcgaccaa cccaccgtcg cgttgctgaa cgcgctgatc gtgcgcacct atgagaacga
      121 aaccggttca cacgcgaaag cactgccatt tgcgcgtgaa gctttcctgt cgacaccggc
      181 caatgccgct tgcgcccttg ccacacgcgc aaggcactac ggcgagctcc tgcttgccct
      241 cgaacgcgat gcggaagcgt tgccggtatt aaagaaatcg cttgaacacg tcaacacctg
      301 cggcgaccgt cttcagaaag cggagatcct gcgcgacctg ggtattttga gctaccgctc
      361 cggccgatac gaggaggcgc tgcgttactt cgagaaaagt cagcaagccg atcccaaact
      421 ccctgccctg cgcctgatgc gcgacagtta cctcaaactc tatgcgggaa gccggatcgc
      481 gcaggacagc agccgcagcc aatactattc tcgcctttat cgcctgttca aagactccgt
      541 tgaacagctc ctgaacagtc gcatgctcag tccggactcg ttcacccgtg aactggcgga
      601 gaaatcccgg gtattggaat tgatcgagcg tcccaccgcg ctggaaggcc gcgccctcag
      661 tacggatcaa ctggcctaca accagcagct caccgaagcc gaactcgagc gcctgaagac
      721 ggaagaagcc ctggcccgga tgaaccagga gaagatgcag gacaaggtcg ccgacaacga
      781 acgcgaagaa aagatccggg aactggagcg acaacaggcc ttgcaggaac tggaactttc
      841 caaaaaagcg ttacgggaat cccgccaacg tcaactcatc aacttgttgc tggccggggt
      901 ggtgttgatc atcgcctttc tcgggttcct cgcttatcgc ttccgggtta acaagaaggc
      961 tcacctcaag ctggaccgcg cttacagcga attgaagaag acccaccaac aactcaaggc
     1021 gacgcaggaa caactggtcc acagcgagaa gatggcttcc ctcggacaga tgaccgccgg
     1081 catcgcgcac gagatccaaa atccgttgaa cttcgtaaac aacttcgcgg aatcttcggc
     1141 ggaattactt gaggaactcc gtcaggccgg cagcaaggaa gaacaggaag aactgctctc
     1201 ggaccttcag atcaacctcg gaaagatcgt ccaccacgga aagcgcgccg acggcattgt
     1261 aaagagcatg ctgcagcaca gccgcggcgg cgacccggtg aagtcgatgg tgcagctcaa
     1321 caagctggcc gaagagtacc tgcacctcgc ctatcacggt atgcgggcca aggatcccgg
     1381 tttcaactgt cggctggaga aacaactcga ccccgcgctt ccggaagttc cggccgtgcc
     1441 gcaggagatc gggcgggtat tgctgaacct gttgagcaac gcgttctacg cggtcggaga
     1501 acgtacggag cgtctacggt cctccgacga agtctatcat ccggaagtcc gcgtttcgac
     1561 ggtatcgctt tccagatatg tcgccctctc ggtaggcgac aacggcaccg gtattcccgc
     1621 cacggttcgc gaacgcatct ttgaaccgtt ctttaccacc aaagccacgg gacaaggaac
     1681 aggacttgga ctctccatca gttacgacat tgtgaaagcg catggcgggg agatgaaagt
     1741 ggaaacggaa gaagggcgcg gcaccatctt cacgctcctg ctgccgatcg cataaaaaaa
     1801 agaagccgct ccttccggaa cggcttctga aaaggaagct tgtcggctta cttcaggtgc
     1861 ttggagacga gtttagtcat ctcgaacatc gaaacggtct tcttaccgcc gaatactttc
     1921 ttcagggcct cgtcagcgtt gatgttacgc ttctgcttgg catcctggag tttgttcttc
     1981 ttgatgtaag cccaaagttt cttaacgact tccgtgcggg gaagcggctt gctgcctacg
     2041 accggctgga gcgcttcgcc gatggcgagc ggacgcatga aagcggcgtt cggtttacgc
     2101 ttggtcttag cttttgcttt tgccttcggc ttagccttag ctacggcttt ctttgccttc
     2161 ttggcgggag cttttttctt ggcttttttg gttgccatgg ttttggattt gatgatgttt
     2221 tgtgaataca aatcaacacg cattccacgt acgcgagacg gcacgagcga agaagttatt
     2281 cacaatccct gtcaattctt cataggggaa atctccggag agtcgcgccc ggcgcagggt
     2341 tccggacctt cgccgcccgt cctccgaatg acgccgctca tccgcagcat ccgccattcg
     2401 tacaagggcc gtggcgcggg acgtcttaac agattattct tgcgatgaaa tgtcaaaatc
     2461 cgctctggga gcggctttcc aaagtcttgg aagattgcat cacgtaaact aatattgtaa
     2521 taggacaacg cagccgttgt cactccccgc taccgacatg aagcattttt ccctcctgtt
     2581 tttcattctg gcttcactgt tcctgccgac ccatacccgt gcgcagcaag acagcctgtt
     2641 gaatacttcg cgtgtcggct gcctctcggg cgactgcgaa aaaggcatcg gcacctaccg
     2701 gaactcggcc ggaagcgttt ataccggacc gttctcggag ggaaagttcg aggggaaagg
     2761 aaaaatgatc ttccgcgacg gtgatgttta cgagggagat ttccaacgcg gcaagatgac
     2821 cggctcgggc acctacacct tcaagagcgg gaccagctat acgggccagt tcaccgacgg
     2881 caagatggat gg
//
LOCUS       DAOTON010000311         2889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26949_length_2889_cov_2.818631, whole genome shotgun sequence.
ACCESSION   DAOTON010000311 DAOTON010000000
VERSION     DAOTON010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26949_length_2889_cov_2.818631"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1354
                     /locus_tag="P5292_06940"
     CDS             119..1354
                     /locus_tag="P5292_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRS38901.1"
                     /translation="MNLTHHDIVVLFLSIGIILLISRILAETGRRFGLPVVMGELIVG
                     ILLGPTVFGSLAPDLQQQLFPTSGSGAVALDGIVQLSVVFLLFVAGLEVQLPIVLRQG
                     RLALFTSFFSLVIPFALGFWITWHFPEWMGYHAGPSRLVYALFMGTALAISALPVIAR
                     ILMDLNLYKTRIGMLIMAAAMFSDLAGWLIFSVVLALLENRGVGTDLGLTILYIFLFG
                     ILMLTVGRKLLNRFVPFAQHKLSWPGGILAFAMGFCLICAAFTASVGVHAILGAFIFG
                     IALGDCVHLHGRAREIIHQFITNVFAPLFFVSIGLKVNFLAHFEPVTVLLVLVLATAG
                     KLVGSTGGAWLGGMDFRSALAVGFGMNARGAMEIILGTLAFQAGLITPEIFVALVVMA
                     LLTSLSSGPLIKATLSGKN"
     gene            1356..1871
                     /locus_tag="P5292_06945"
     CDS             1356..1871
                     /locus_tag="P5292_06945"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRS38902.1"
                     /translation="MRIYLLGYMGAGKSHLGKILAERLGYSFVDTDAMVEATTGKSIR
                     QLFEEHGEGAFRIAEQKALHATGQQTDLVVATGGGLPTFADNLAWMNNNGMTVYLQLS
                     PGTLFHRLVREREHRPLLAGIGEIDLMETIHRHLIERSPYYQQARLVIDPEQVSAGEL
                     VAMIMHRLDGG"
     gene            complement(1868..2368)
                     /locus_tag="P5292_06950"
     CDS             complement(1868..2368)
                     /locus_tag="P5292_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRS38903.1"
                     /translation="MQATTILNATQSEQRIQRLAWQLYEDNYTEKEILLIGILQNGYP
                     LAERIAAALRKIAPFQVHLFSLKIDKHSQDVGEPVIAPSLPALQDKVVVLVDDVLNSG
                     KTLIYALKPFLRADIRKLRTVVLIDRNHRRYPIAADFVGLSLATTLQEHVQVTFQGES
                     AVAELS"
     gene            complement(2373..2864)
                     /locus_tag="P5292_06955"
     CDS             complement(2373..2864)
                     /locus_tag="P5292_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRS38904.1"
                     /translation="MSLSAATPRLLPGQDTDPEQQQLEWCFIYSNSLGYNIDYINNPR
                     LYEKVKQWLGTPYRYSGETMNGIDCSGFVCQVYKDVYDKILAGSSRDIHKNTVKIRED
                     ELREGDLVFFKIRKSRVSHVGIYLGQNKFAHASVQRGVIISDLSEDYYRKYFYSAGRL
                     KTN"
BASE COUNT          583 a          758 c          813 g          735 t
ORIGIN      
        1 gcccggacca tcatctaagc atcttcgacc ggatcttcac gcacgatctt gaatacctgc
       61 tttccgacat gccttgcagt ctgctgatcg ttcattcccg aatgccggaa cagcccctat
      121 gaacctgact catcacgaca tcgtcgtgtt gtttctttcc attggaatca tcctgctgat
      181 ctctcggata ctggccgaga ccggtcgccg tttcggattg ccggtggtga tgggtgagtt
      241 gatcgtcggt atactgttgg ggccaacggt attcggatcg ctggctcctg acctgcaaca
      301 acaacttttc ccgaccagtg gaagcggtgc cgtcgcgctg gacgggatcg tacaattgtc
      361 ggtggtcttt ctgctctttg tggcaggact tgaggtgcaa ctgccgatcg tactgcgaca
      421 aggccggctg gcattattca ccagcttttt ttccctggtg atacccttcg cgctcggatt
      481 ttggatcacc tggcatttcc ccgaatggat gggctatcac gcgggaccgt cacgactcgt
      541 ctacgccttg ttcatgggta ccgctttggc catttcagct ctcccggtca ttgccaggat
      601 cctgatggat ctgaaccttt acaagacacg gatcggtatg ctgatcatgg cggctgccat
      661 gttcagtgac ctggctggct ggctcatctt ttccgtggta ctggccctgc tggagaaccg
      721 tggagtcggg accgatttgg gtttaacgat cttatacatc ttcctgttcg gcatactcat
      781 gctgacggtc ggacgtaaac tgctcaaccg tttcgtaccg tttgcgcagc acaaactttc
      841 ctggcccggc gggatcctgg cttttgccat ggggttttgt ctgatttgcg ccgcgtttac
      901 agccagtgtc ggcgtgcatg cgatccttgg ggccttcatc ttcggtatcg cgctgggtga
      961 ttgcgtgcac ttacacggac gagcccggga aatcattcat cagtttatta cgaacgtctt
     1021 tgcacccttg ttttttgtca gcatcggatt gaaagtgaat tttctcgcgc actttgaacc
     1081 ggtaacggta ctactggtac tggtactggc tacggcgggg aagttggtcg gatccacagg
     1141 cggtgcctgg ctgggcggaa tggatttccg gagtgcactg gctgtaggct tcggcatgaa
     1201 cgcccggggc gcgatggaga tcatcctggg tacgcttgcg tttcaggccg gcttgataac
     1261 gcccgaaatt ttcgtcgccc tggtcgttat ggccttgctt accagtttga gtagcggacc
     1321 gctgatcaaa gcaaccttat ccggaaagaa ttgacatgag aatctacctc ctcggctata
     1381 tgggagccgg taagagccat ttaggaaaga tactcgcgga gcgcttgggt tattcctttg
     1441 ttgacacgga cgccatggtt gaagcgacaa caggaaaatc aattcggcag ctattcgaag
     1501 agcatgggga gggcgccttt cgtatcgcgg agcagaaagc attgcatgct accgggcaac
     1561 aaaccgacct ggtggtagcc accggtggcg gattgccgac tttcgccgat aacctggcat
     1621 ggatgaacaa taacggcatg accgtttacc tgcagctttc gcccggaacc ttgtttcatc
     1681 gactcgtgcg cgaacgggag catcgaccac tcctggccgg cattggagag atcgacctaa
     1741 tggaaaccat tcaccgccac ctgatcgagc ggtcgccgta ttatcaacaa gcccgattgg
     1801 tgatcgaccc ggagcaagtc agcgccggtg aattagtggc gatgatcatg caccggcttg
     1861 acggtggtta agagagttcg gcgacagcgc tctcgccctg gaacgtcacc tggacatgtt
     1921 cctgcaaggt ggttgcaagg gaaagcccca caaagtccgc ggcgatcggg taccgtcgat
     1981 gattacgatc gatcagcaca accgttcgta gtttgcgtat gtcggcacgg agaaacggtt
     2041 tgagcgcgta gatcagggtc ttcccggagt tcagtacgtc gtccaccagc acaaccactt
     2101 tgtcttgcag cgcgggaagg gagggggcga tcaccggttc gcctacatcc tggctgtgtt
     2161 tatcgatctt cagactgaag aggtgcacct ggaaaggggc gatcttccgc agggccgctg
     2221 caatgcgttc tgccagcggg tatccattct gtaagatgcc gatcagcagg atctcttttt
     2281 ccgtgtaatt gtcttcgtac aactgccagg caagtcgttg aatgcgttgc tccgactggg
     2341 ttgcgttcag gatggtcgtg gcctgcatgg ggtcagttgg ttttgagccg accggctgaa
     2401 tagaagtact ttcggtaata gtcctccgac agatcggaaa tgatcacgcc gcgttgcacg
     2461 ctggcatgcg cgaacttgtt ttggccgagg tagatcccga catgagaaac gcggcttttc
     2521 cggatcttga aaaagaccag gtcgccttcc cgcaattcat cttcccggat ctttaccgtg
     2581 tttttatgaa tatcgcggga actgcctgcg agaattttat cgtaaacgtc cttatatacc
     2641 tggcaaacga atccggagca gtcgatgccg ttcatcgttt ccccggaata acgatacggc
     2701 gttcccagcc actgcttcac tttttcatac aagcggggat tgttgatgta atcgatgttg
     2761 taacccagac tgttggaata gatgaaacac cattccagtt gctgctgttc gggatcggta
     2821 tcctgtccgg gcaacagccg gggcgttgca gccgataagg acatggaaag cggccctcaa
     2881 tcaattggc
//
LOCUS       DAOTON010000312         2886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_26984_length_2886_cov_3.613917, whole genome shotgun sequence.
ACCESSION   DAOTON010000312 DAOTON010000000
VERSION     DAOTON010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26984_length_2886_cov_3.613917"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /gene="hemE"
                     /locus_tag="P5292_06960"
     CDS             complement(<1..1020)
                     /gene="hemE"
                     /locus_tag="P5292_06960"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRS38905.1"
                     /translation="MSLKNDLLLRAARGQATERTPVWLMRQAGRILKEYREVRQRVGG
                     FKELVEHPEFAAEVTIQPVDLLGVDAAIIFSDILVIPEAMGLSYQVIEAKGPTFEQTV
                     RSKSDIEKLRVAGPEHLSYVLEAIRITKRELHDRVPLIGFAGAPWTILAYMVEGKGSK
                     TFSVAKKFLYTEPALAHALLEKITESTIQYLKAQIAAGADLVQLFDSWAGLLSPDQYR
                     EFSLRYLEAIAAAITEVPVTIFAKGAFFARKEIAATNCSVIGLDWNMDIQESRALIGD
                     AKTLQGNMDPCLLYAEDSVIVHETHRMLEAFGPSRHIANLGHGLYPDTDPRKVKLFID
                     TIKNFR"
     gene            complement(1116..>2886)
                     /locus_tag="P5292_06965"
     CDS             complement(1116..>2886)
                     /locus_tag="P5292_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRS38906.1"
                     /translation="IYQRLIRLILLATGVLLSAATCAQKKSQFALSIVWHSLPEGRTE
                     ADLRKSYVPERVISDSASGVNILHRAIRQLRTDGYLCAGLDGFSRTDEGYRGELYVGS
                     SYTWLQLHRGDAEESYLRGTGYRNRWLKEQVYSPEKLDRILENIIRNCENAGYPFATV
                     RLDSLSLSGDLLEAALRLEKGPFVRIDSIRIQGTARIAPVYIHNYIGIQSGDPYDERL
                     VRRISNRMKELPFLTEARGSQVVFAEKITRLDLYLNEKKASQFDAVIGFLPDESRPGK
                     LNLTGEAHLRLQNSFQRGEVVELNWKQLPPRSQDLKLHLQYPFLFNTALGVDGNLNLF
                     KRDTVFTDVVKNIGVFYSFTGNNFIKAFLNDKQSNLQSTSGLENITVLPPYADISLLS
                     YGLTGHFERLDYRLNPRTGFAIEASVSTGNRNIRKNADINPLVYDSLKLRTVQYIGEM
                     KLDVYLPVSGRLVLNLGSIGAYQYNPEAFTNELFRIGGLRSLRGFDEESIFSSAFVIG
                     KTELRYLLEQNSFLFVFGNTAWYERRRKQEYRNDWPIGFGTGINFETRLGILSVSYAL
                     GRQAENPFQIRNGKVHFGIVNYF"
BASE COUNT          650 a          762 c          777 g          697 t
ORIGIN      
        1 ccggaaattc ttaatggtgt cgatgaatag tttcactttc cgaggatccg tgtccggata
       61 aagaccgtgt ccgagattgg caatatggcg ggatggtccg aatgcttcga gcatacggtg
      121 ggtttcgtgt acgattacgg agtcttccgc gtagagcaga cacggatcca tattcccttg
      181 aagcgttttg gcatcaccga tcagcgccct ggattcctga atatccatgt tccagtcaag
      241 cccgatgacc gaacagttgg tcgcggcgat ttctttccgg gcaaaaaatg cccccttggc
      301 aaagatcgta acgggtactt cggtgatagc cgctgcaatc gcctcaagat aacgcaggga
      361 gaattcccgg tattggtccg gtgacaagag cccggcccag gaatcaaaca gttgaacaag
      421 atcggccccg gcggcgatct gtgccttgag atactgaatc gtgctttccg tgatcttctc
      481 cagcaatgca tgggcaagcg ccggttcagt gtaaaggaat ttcttagcaa cggaaaatgt
      541 cttgcttccc tttccctcga ccatataggc gagaatggtc cagggtgcgc cggcaaaacc
      601 gatcaggggt actcgatcat ggagctcccg tttggtgatt cggatggctt ccagaacata
      661 ggaaagatgt tccggccccg ccacacgtaa cttttcgata tcggatttgc tcctgaccgt
      721 ctgttcaaag gtcggcccct tcgcttcaat gacctgatac gataagccca tcgcttcggg
      781 aatgaccagg atatcggaaa agatgatcgc cgcgtcaaca cccagcaagt cgacaggttg
      841 aatcgtgact tccgcggcga attcggggtg ttcgaccaac tccttgaaac cacccacgcg
      901 ttgccggact tcccggtatt ctttcaggat acggcctgcc tggcgcatca gccaaacggg
      961 ggtgcgttca gtggcctgac cgcgcgcagc ccgtagcaga agatcgtttt tcaatgacat
     1021 agggccgcaa agttagccaa ttgcgggaat ggactcttca gatggcgctg cttctgtcat
     1081 cagattccca tctttccgca tctatcgggc tttctttaaa agtagttgac gatgccgaag
     1141 tgcactttcc cgttacgaat ctggaaaggg ttttctgctt gtcttccgag cgcgtacgaa
     1201 acggacagga tacccagccg ggtttcgaaa ttgattccgg ttccgaaccc gataggccaa
     1261 tcgtttcggt attcctgctt gcgcctgcgc tcataccagg cggtgttgcc gaacacgaag
     1321 agaaaggagt tctgttcaag caggtaacgc agctctgttt taccgatcac aaaggcagag
     1381 gagaagatcg actcttcatc gaagccgcgc agcgagcgca agcctccgat acggaacaat
     1441 tcgttcgtga aggcttccgg attgtattgg taggctccga tactaccaag attcaatacc
     1501 agtcttccgc tcaccggcag atatacatcc agcttcattt cgccgatata ctgcacggtg
     1561 cggagtttca aactgtcgta taccagcgga ttgatgtcgg cgttcttgcg aatgtttcgg
     1621 ttgccggtac tgacgcttgc ttcgatagcg aatccggttc gtggattcag gcggtaatcc
     1681 agtcgttcga aatgtccggt cagcccatag ctgagcaggc tgatatcagc gtaaggaggc
     1741 aatacggtga tgttctccag cccgctggtg gattgaagat tgctctgctt gtcgttgagg
     1801 aacgctttga tgaaattatt acctgtaaag ctgtagaata ccccgatgtt cttcactacg
     1861 tcggtgaaga cggtatcacg cttgaagagg ttaaggttgc cgtcaactcc caacgccgtg
     1921 ttgaagagga atggatattg aagatgcaat ttcaggtcct gtgaccgggg cggtagttgt
     1981 ttccaattca gctccacgac ctctccgcgt tgaaacgaat tctgtaatcg caaatgcgct
     2041 tcgccggtga ggttcagttt ccccgggcgg gattcgtccg gaagaaaacc gatcactgca
     2101 tcgaactggc ttgccttctt ctcattcagg taaaggtcaa gccgggtgat cttttcagcg
     2161 aacacaactt gcgatcccct ggcttccgta aggaacggca actctttcat ccggttgctg
     2221 atccggcgga ccaaccgttc gtcataggga tcaccgctct ggatcccgat gtagttatga
     2281 atgtacaccg gcgcgatccg ggcggtaccc tggatgcgga tgctgtcgat cctgacaaag
     2341 ggacccttct ccaatcgcaa cgcggcttcc aataagtcac cggacaacga caggctgtcg
     2401 agtcgtacag tcgcgaacgg ataccccgcg ttttcacaat tgcggatgat gttttccagg
     2461 atgcggtcaa gcttttccgg actgtagacc tgttccttca gccatcggtt gcgatatccg
     2521 gttccgcgca agtacgattc ttcggcatcc ccgcggtgaa gttgcagcca ggtatacgat
     2581 gagcccacgt agagctcgcc cctgtaaccc tcgtcggtac ggctgaatcc gtcaagaccg
     2641 gcacacaaat aaccgtcggt gcgcaattgc cggatggccc gatgcaggat gttgacaccg
     2701 gaagcggagt cggaaatgac acgctccgga acatagctct tccgcaagtc cgcttcggtt
     2761 cgtccttccg gtagtgaatg ccatacgatg gataacgcga actgactttt cttctgtgcg
     2821 catgttgccg cggaaagaag aacgccggtt gccagtaaga tcagccggat gagccgttgg
     2881 taaatg
//
LOCUS       DAOTON010000313         2878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27073_length_2878_cov_3.136734, whole genome shotgun sequence.
ACCESSION   DAOTON010000313 DAOTON010000000
VERSION     DAOTON010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27073_length_2878_cov_3.136734"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="P5292_06970"
     CDS             complement(<1..669)
                     /locus_tag="P5292_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015158575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS38907.1"
                     /translation="MSRLISRNVLLLSLISLCTDLASELLYPVMPGYLRSIGFSMALI
                     GLLEGIAEAIAGLSKGYFGHLSDVKGRRLPFVQWGYALSAISKPLFAITVRPVGVFFA
                     RTIDRVGKGIRTGARDALLSADASVATKARVFGFHRAMDTIGAVLGPLVALLYLSYHP
                     GAYRHLFLLAFLPGFGAIVLTMFLRERRAQNQAKTVSPVRFLDFTRYWRIADPAYRKL
                     VAALL"
     gene            827..901
                     /locus_tag="P5292_06975"
     tRNA            827..901
                     /locus_tag="P5292_06975"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:862..864,aa:Val,seq:tac)
     gene            950..1159
                     /locus_tag="P5292_06980"
     CDS             950..1159
                     /locus_tag="P5292_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRS38908.1"
                     /translation="MYILYSKKADRFYIGSTAYPVEQRLRKHLTNHRGYTSRFKDWVI
                     AYKELYGTYNEARKREMELKRSKDR"
     gene            complement(1332..2633)
                     /locus_tag="P5292_06985"
     CDS             complement(1332..2633)
                     /locus_tag="P5292_06985"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRS38909.1"
                     /translation="MTYPEVLDYLYSCLPMFHRVGAVAYKANLDNTHHLMELLDHPEQ
                     GFRSVHVAGTNGKGSSSHMLAAVLQAAGYKTGLYTSPHLLDFRERIRVNGGMVPEEVV
                     TGFVQAHRAAFDRIQPSFFEWTVALAFDYFRNEQVDIAIIETGLGGRLDSTNVITPEV
                     ALITNIGWDHMNLLGDTLEKIAAEKAGIIKPGVPVIISEGQLETIHVLTSAAQRAGSN
                     SCVADQEYKVEAIGGDGRDLNVSVEREGAVQLHYTIGLPGRYQTKNVAGVLATIYRLR
                     EMGWKIPEAAVARGLRDVRSLTGLMGRWQLLKEQPKVIADVGHNLDGIREILLQLEHE
                     NHTDLHWVIGVVEDKDLSGILPLLPAAATYYCCKPDLPRGRDAASLCQALREHGLRAE
                     AYPSVALALASAMRSANERDLILIAGSTFVVAEAMKASQIS"
     gene            complement(2677..>2878)
                     /locus_tag="P5292_06990"
     CDS             complement(2677..>2878)
                     /locus_tag="P5292_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRS38910.1"
                     /translation="GKVVVEITVDKNGHVTSAIPGGRGSTTTSSYLYRLAKEAAMKAE
                     FSPSPDGADIQKGTMTFIFLVQ"
BASE COUNT          720 a          797 c          733 g          628 t
ORIGIN      
        1 cagcagcgct gcgacgagtt tacgatatgc cggatccgca attcgccaat agcgagtaaa
       61 atcaaggaac cgaaccggcg aaaccgtttt tgcctgattc tgggctcttc tttcacgcag
      121 aaacatggta agtacgatag ccccaaaacc aggcagaaac gcaagtagaa acaagtgtct
      181 atatgcgccc gggtgataac tcaaatagag caaggcaacc agcggaccaa gtaccgcacc
      241 gatcgtgtcc attgctcgat ggaatccgaa cacacgagcc tttgttgcaa ccgaagcgtc
      301 agccgacagc aatgcgtcgc gggcaccggt tcgtatccct ttgccgacgc gatcgatggt
      361 ccgggcaaag aagacgccaa ccggacgaac agtaatcgcg aacagaggtt tggaaatggc
      421 actcaaagca taaccccatt ggacaaatgg cagcctgcgc cccttcacat cgctcagatg
      481 tccgaaataa cctttactca aacctgctat cgcctccgca atgccttcca gcaatccgat
      541 caacgccatg gaaaaaccaa tcgaacgcag gtatccgggc atgacaggat acaaaagctc
      601 actggcgagg tccgtacaca ggcttatcag agaaagcagc agaacgtttc tggatatcaa
      661 tctggacatc gtatgcttat aatccgggtt ggaacgctga acttcgtttg aagcgaaaaa
      721 atacgtccaa gtttttagga aatgatcgaa acagctatat ttgcactcct ttttcaacaa
      781 aatccgccga ctttgattgt gctaaactgt tgaaaaagag atcattggga gcatagctca
      841 gctggttcag agcatctgcc ttacaagcag agggtcacag gttcgaaccc tgttgctccc
      901 acaaaacgga aacccaccct tgcggtgggt ttctgcattt atggggtagt tgtatatact
      961 atacagcaaa aaagctgatc ggttctatat cgggagcacg gcctatcctg ttgagcagcg
     1021 gttgcgcaaa cacctcacga atcacagagg ttatacatcc cggttcaaag attgggttat
     1081 cgcatacaag gagctttatg gaacatacaa cgaagccagg aaacgggaaa tggaactgaa
     1141 gcgatcgaaa gatcgctagt tactcgagcg attgatttca gcaggggcag agcatcccgg
     1201 ctaacagccg ggagggtcac agagcctgtc ccggtttacc gggattcgaa ccctgttgct
     1261 cccacaaaac ggaaacccac cctttttttt atcggctgtt tgggcaccag ctagcctctc
     1321 tggctaaacc atcagcttat ttgacttgcc ttcatcgcct ccgctactac aaaggtgctg
     1381 ccggcgatga gtatgagatc ccgctcgttc gccgatcgca tggcggaggc taaggccagg
     1441 gctacgcttg gataggcttc tgcgcgaagt ccgtgttcgc gtagtgcctg gcacagggat
     1501 gccgcatcac gtcctctggg caagtccggt ttgcaacagt agtaagtggc tgcggcaggc
     1561 aacaagggta gaatgcccga aaggtctttg tcttcaacca caccgatcac ccagtgtaag
     1621 tccgtatgat tttcatgctc caattgtagg aggatctccc gaatgccatc aaggttgtga
     1681 cctacatccg cgatcacctt cggttgttct ttcagcaact gccagcgtcc catcaaaccg
     1741 gtcagactcc tgacatcccg taatccgcgc gcgactgcag cctccggtat cttccagccc
     1801 atttcgcgca atcgatagat cgtcgctaaa acacccgcga cattcttggt ctggtaccga
     1861 cccggcaatc cgattgtata atgcaactgc accgcacctt cacgctcgac cgacacgttc
     1921 aggtcgcgcc catctcctcc aatcgcttca accttgtact cctgatccgc aacgcagctg
     1981 tttgaaccgg cgcgctgagc cgcggatgta aggacatgga tggtttcaag ctgaccttcg
     2041 ctgatgatca ccggcactcc cggtttaatg ataccggcct tttccgccgc gatcttctcc
     2101 agggtgtcac ccagcaaatt catgtggtcc caaccgatgt tggtgatgag tgccacttcc
     2161 ggagtaatca cattggtaga gtccaaccga ccgcctaagc cggtctcgat gatggcgatg
     2221 tcgacctgtt cgttcctgaa ataatcgaac gccagggcta ccgtccattc aaagaaagaa
     2281 ggctggatgc gatcgaaagc ggcgcggtga gcttgaacga atccggtcac cacctcctcc
     2341 ggaaccatgc caccgttgac acggatgcgc tcacgaaagt cgagtaagtg cggcgatgta
     2401 tacagaccgg tcttgtaacc ggcggcctgc agaaccgctg ccagcatatg cgaactggag
     2461 cccttaccgt tcgttcctgc cacgtgcact gaacggaatc cctgctcggg atggtccagc
     2521 aattccatca ggtggtgtgt gttgtccagg ttagccttgt aggccaccgc gccaacccga
     2581 tgaaacatcg ggagacagga atacaggtag tcgaggacct cgggataggt catagccggc
     2641 aacaaagaaa agaaggattc ccctacccga agcgggttat tgtacgagga agatgaaggt
     2701 catggtcccc ttctggatat cggctccgtc gggactggga ctgaattcgg ctttcatggc
     2761 ggcctctttc gccaggcgat acagatacga gctggtggtc gtagaaccac gaccgccggg
     2821 gatggcgctg gttacatgtc cattcttatc aacggtaatt tcgacgacca ccttgccg
//
LOCUS       DAOTON010000314         2870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27175_length_2870_cov_3.248313, whole genome shotgun sequence.
ACCESSION   DAOTON010000314 DAOTON010000000
VERSION     DAOTON010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27175_length_2870_cov_3.248313"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="P5292_06995"
     CDS             complement(<1..430)
                     /locus_tag="P5292_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRS38911.1"
                     /translation="MVDLFEKIKKNRGPIGQHAKDSHGYFTFPKLEGEIGSKMVFRGK
                     ERLVWSLNNYLGLANHPDVRKADAAAAAEYGFASPMGARMMSGNTNYHEQLENELSAF
                     MHKEDTILLNFGYQGMVSAIDCLVDRHDVIVYDSESHACII"
     gene            774..>2870
                     /locus_tag="P5292_07000"
     CDS             774..>2870
                     /locus_tag="P5292_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HRS38912.1"
                     /translation="MKKLIAFLGCILLLSVSSLSAQNLQLRANLSYGSSALANIGGYV
                     DHDGNEYALVGTDFGLSIVDVTDPDNPAILFTVPGANSIWREVKTYHDFAYVTTEGGG
                     GLTIVDLSQLPANVNYYDYNGDGAIANQLETIHALHVDTTKGFLYLYGSNIGSGNTLF
                     FDLSDPANPTYAGEYVYPGGGSDAYVHDGYADNDTLYQSHIYAGFFTVVDVTDKANPV
                     LLATQVTPTEFTHNTWLSVDHKTLFTTDENSGSFLGCYDISDLSNITELARFQTAPGS
                     GAVIHNTHILNDFAVTSWYKEGVVITDVSRPANPVEVGHYDTYAQGSGNGFNGCWGVY
                     PFLPSGTVVASDIDNGLFVLTPTYVRACYLEGKVTDSITGFPLQAASVRLLTTTLTAV
                     SDILGDYRTGTVTGGTYDVEISKPGYQTKTISGVTLSNGVLTTLDVQLAPVPTISISG
                     TVLDSLTGQPIAGAMVMMSDPDFTFNTTTDAAGTFSLNGVVAGNYDVTVGLWGYVTKC
                     SGFVAGPGAFQTELVPGYYDDFTFDFGWTVSGTSPNAWERGIPVGTYDNQGDEVNPAT
                     DVSGDCQQYCYVTDNGGGTFSNHDVDNGNTQLTSPVFDATIYSNPTIEYYRWFVNTAG
                     TGTPNDLMNFRLTNGTTTIALEQVSITSPGQGTWVPHAIPLTGLLPLTSTMQLIVDIA
                     DDAPGHVLEGGLDRFSI"
BASE COUNT          669 a          773 c          708 g          720 t
ORIGIN      
        1 caatgatgca ggcatggctt tcgctgtcgt aaacgattac atcatgacgg tcaaccaggc
       61 aatcaatcgc tgaaaccatt ccctggtatc cgaaattcag cagaatggtg tcctccttat
      121 gcatgaaggc ggaaagctcg ttttccaact gctcgtggta gttggtgttg cccgacatca
      181 tgcgtgctcc catcggggaa gcaaatccat attctgcagc ggcagcggca tcggccttgc
      241 gaacgtcggg atggttggcc aagccgagat aattattcaa actccaaaca aggcgctctt
      301 ttccacggaa taccattttc gaaccgattt caccttccag ttttggaaag gtgaagtagc
      361 catgcgagtc tttcgcgtgc tgaccgatcg gaccgcgatt cttcttaatt ttttcaaaca
      421 gatccacgat gttctccttt ttgtcgaggc cttccgatga aggccttctc tacaaatgta
      481 accataattg ctcgaattca aagaatttta aggcttttca tcaacacgtc agtgtggaag
      541 aagtaggtgc ggagctgatc acatgcatta gaaacagttc accatttgct atatatatta
      601 ttgatattca gatatataaa gcttatttca gtagcctcca tacatcgttc cagcggcaga
      661 tttgttggtt tcaatctccg atcccgcagg acttgcatgg acaaatttga cgctaaaaag
      721 acatggactt cattgtattt acctgaaaat ctgctgtcct tgcagcccca cccatgaaaa
      781 agctcatcgc cttccttggc tgcatcctgc tgctgtcagt ttccagcctc tccgctcaaa
      841 acctccaatt acgtgcgaat ctgtcttacg gctcctccgc attggccaac atcggaggtt
      901 atgtagacca tgatggaaac gaatatgccc tggtcggaac agatttcggc ctttcgatcg
      961 tcgacgttac cgatcccgac aatccggcaa ttctttttac tgttcccggc gccaattcca
     1021 tttggcgtga ggtcaagact taccacgatt tcgcttatgt aacaacggaa ggtggcggag
     1081 gcttgaccat cgtcgacctt tctcagcttc ccgcaaacgt caattattac gactacaacg
     1141 gggatggggc gatcgccaac caattggaaa cgatccacgc cctgcacgtc gatacgacca
     1201 agggattcct gtatctctac ggcagcaata tcggaagtgg aaacaccctg tttttcgact
     1261 tgtccgatcc cgccaacccg acctacgcag gggaatatgt ttaccccggt ggcggcagcg
     1321 atgcctatgt acacgatggc tatgccgata atgacacctt gtatcagtca cacatctacg
     1381 ccggcttctt caccgtcgtg gatgtaaccg acaaggcgaa cccggtgctc ctggctactc
     1441 aggttacccc gacggaattc acgcacaata cctggctttc ggtcgatcac aagaccttgt
     1501 ttaccacgga cgagaattct gggtcctttc tcggctgcta cgatatttcc gatttgagca
     1561 atatcacgga gctggcacgc tttcagaccg ctcccggcag cggtgccgtt attcataata
     1621 cgcatatcct gaacgatttt gccgtgactt cctggtataa ggagggggta gtcattaccg
     1681 acgtttcccg tccggcgaat ccggtcgaag taggacacta tgatacctac gcacaaggaa
     1741 gtgggaatgg attcaatggc tgctggggcg tttatccgtt cctgccgtcg ggtacggtcg
     1801 tcgcgtccga catcgacaat gggttgtttg tcctgactcc gacctatgta agagcctgct
     1861 acctggaagg aaaggtcact gacagcatta ccgggttccc tttacaagcg gcaagtgtcc
     1921 gattgctgac caccaccctc acagctgttt ccgatattct cggcgattac cggaccggga
     1981 cggtaaccgg tggtacatac gatgtcgaga tcagcaaacc cggctatcag accaagacaa
     2041 tttcaggtgt caccctttcc aatggtgtgc tgacgacgct cgatgtacaa ttagcgcccg
     2101 tgccgaccat cagtattagc ggaacggtat tggatagtct gacgggtcag cccatagccg
     2161 gtgccatggt gatgatgtcg gatcctgact tcacgttcaa caccaccaca gatgctgccg
     2221 gaactttctc cctaaacggt gtggtagctg gaaattacga tgtcacggtc ggattgtggg
     2281 ggtatgtgac caaatgttcg ggcttcgttg caggccccgg agcctttcag acggaactgg
     2341 tacccggcta ctacgatgac ttcacctttg acttcggttg gaccgtgagt ggcacttctc
     2401 ccaatgcgtg ggagcggggc attccggttg gcacatacga taaccaggga gatgaagtca
     2461 acccggctac ggatgtatcg ggcgattgtc agcagtactg ttacgtcacc gataatggtg
     2521 gaggcacatt ctccaaccac gatgtcgaca acgggaatac gcaactgacc tcaccggttt
     2581 ttgatgccac catctattcc aatcctacca tcgaatacta tcgctggttt gtcaatacgg
     2641 cgggtacagg gacgccgaat gacctgatga acttccgtct gacgaatgga acaacgacga
     2701 tcgccctgga acaggtcagc atcacctcac cgggccaggg tacctgggtt ccacatgcga
     2761 ttccgcttac cggtctgctg ccgttgacct ccaccatgca gttgatcgtc gacattgcag
     2821 acgatgctcc ggggcatgtg ctggaaggcg gactggatcg gttcagcatt
//
LOCUS       DAOTON010000315         2870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27176_length_2870_cov_3.137833, whole genome shotgun sequence.
ACCESSION   DAOTON010000315 DAOTON010000000
VERSION     DAOTON010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27176_length_2870_cov_3.137833"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="P5292_07005"
     CDS             complement(<1..1031)
                     /locus_tag="P5292_07005"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS38913.1"
                     /translation="MRNSRYFLFALLLLGACKSQESATTRVQTPAAKDALTPSQRADV
                     TYLFFNANKEKILGNYNAAADLFAEVIRKDPGNAAAMYELAGIFIEQKKFGDALFFAK
                     SAYSINPANEWYAQTYAEVLQRNRRFTEAAAVWEQQVKRNPDRSDFYLQWADALIFAD
                     KPAEAVKAYDKLEDRTGVNKDISTAKARIYQRMGKNDMAVSELQKLIANDPNDPQAYG
                     MLAEVYQAMGQKEKALETYNRILEIDPDNPFIHLSLADYYRSNGEKEKSVVELKKAFN
                     NPELGIETKISILSSYYALIELHPELREQAMEMTELLVKTHPSDPRAFAVRGDFLIQE
                     KKYEEARVA"
     gene            complement(1052..2056)
                     /locus_tag="P5292_07010"
     CDS             complement(1052..2056)
                     /locus_tag="P5292_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRS38914.1"
                     /translation="MKIIVPMAGMGKRMRPHTLTVPKPLIPVAGKPIVEWLVEDITRT
                     CKEKVDEIAYVIGDFGAEVEKQLIAIAERLGARGTIHYQDEPLGTAHAILCAQSALQG
                     KVIVAFADTLFRAEFTIDESRDGVIWVNRIDDPRMFGVVKLDGEGFITDFVEKPQEFI
                     SDLAIIGIYYFKDGEYLRKELQFLLDNDIREKGEYQLTNALENMKRKGTRFVPGKVEE
                     WLDCGNKDATVHTNKRVLDYQAGAATVAASVKQEHSVIIPPCFIGENVVLKNSVVGPF
                     VSIGANSRIESSVVSNSILQTGVTLKHANVDNSMIGHSAQLHLRPEQLSISDYSTTHH
                     "
     gene            complement(2203..2634)
                     /gene="dut"
                     /locus_tag="P5292_07015"
     CDS             complement(2203..2634)
                     /gene="dut"
                     /locus_tag="P5292_07015"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015267815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRS38915.1"
                     /translation="MNVKIINRSRHPLPAYETAHAAGMDLRAFLETPIELKPLERALV
                     PTGLFLELPVGYEAQVRPRSGLAAKRGVTVLNSPGTIDADYRGEVKVILVNLSNETFR
                     IEDGERVAQLVVARHERVEWTDAEELSLTDRGTGGFGSTGK"
BASE COUNT          527 a          741 c          896 g          706 t
ORIGIN      
        1 taggcgacgc gggcttcttc gtactttttc tcctggatca ggaaatcgcc gcggacggcg
       61 aaggcgcggg gatcgctcgg gtgggtcttg accagcagtt cggtcatctc catggcctgc
      121 tccctgagtt ccggatgcag ctcgatgagg gcgtagtacg aactcaggat actgatcttg
      181 gtttcgatgc ccagctccgg gttgttgaac gctttcttga gttcgacaac cgatttctct
      241 ttctcgccat tgctgcggta gtaatcggcc agcgagagat gaatgaaagg attgtccggg
      301 tcgatctcga ggatgcggtt gtaggtctcg agcgcttttt ccttctgtcc catcgcctgg
      361 tagacctcgg ccagcatgcc gtaggcctgg ggatcgttgg ggtcgttggc gatgagtttc
      421 tgcaattcgg acacagccat gtcgttcttg cccatgcgct gatagatgcg cgccttggcc
      481 gtgctgatgt ctttgttgac gccggtgcgg tcttcgagtt tatcgtaggc cttcaccgct
      541 tcggcgggct tgtcggcgaa gatgagcgcg tcggcccact gcaggtagaa atcggaacgg
      601 tcggggttcc gcttcacttg ttgttcccaa acggcggcgg cttcggtgaa gcggcggttg
      661 cgttgcagga cctccgcgta ggtctgcgcg taccattcgt tggcgggatt gatgctgtag
      721 gccgacttgg cgaagaagag cgcgtcgccg aactttttct gctcgatgaa gatgccggcc
      781 agctcgtaca tggcggcggc attgcccggg tctttgcgga tcacctccgc gaacaggtcg
      841 gctgcggcgt tgtagttgcc gaggatcttt tccttgttcg cgttgaagaa gaggtaggtc
      901 acgtccgccc gttgggaagg cgtcagcgcg tctttcgcag ccggggtctg cacacgcgtg
      961 gtggcagact cctgactttt acaggcgcca aggagcagaa gcgcgaacag gaaatagcgg
     1021 gagttacgca tgcgggcggg ttgataccgg atcaatggtg ggtcgtggag taatcgctga
     1081 tgctcaattg ctcggggcga aggtgcagtt gcgcggaatg cccgatcatg gagttgtcga
     1141 cattggcatg cttcaatgtt acaccggttt ggaggatgct gttgctcacc accgacgact
     1201 cgatgcggga attggcgcca atgctaacga acggaccgac cacggaattt ttcaaaacga
     1261 cattttctcc gataaaacag ggcgggatga tgaccgaatg ctcctgcttg acggaggcgg
     1321 ccacggtcgc tgcgccggcc tggtagtcga gcacgcgctt gttggtatgc acggtggcgt
     1381 ccttattacc gcagtcgagc cactcctcca ccttacccgg gacgaaacgc gtgcctttgc
     1441 gtttcatgtt ctcgagcgcg ttggtaagct ggtactcgcc tttttcccgg atgtcgttgt
     1501 cgagcaggaa ttgcagctcc ttgcgcaggt attcgccgtc cttgaaataa tagatcccga
     1561 tgatggccag gtcggagatg aactcctgtg gtttctcgac aaaatcggtg atgaatcctt
     1621 caccgtcgag cttgaccacg ccgaacatgc gggggtcgtc gatgcggttg acccagatta
     1681 caccgtcgcg gctttcgtcg atggtgaact cggcacggaa cagcgtatcg gcgaaggcca
     1741 cgatgacctt tccctggagt gcgctctggg cacagaggat ggcgtgggcg gtacccagtg
     1801 gctcgtcctg gtagtggatg gttccgcggg cgccaaggcg ttcggcgatg gcaatgagtt
     1861 gcttttccac ttcggctccg aagtcgccga tgacgtaggc gatctcgtcg accttttcct
     1921 tacaggtacg cgtgatgtct tccaccagcc attcgacaat aggttttccg gctaccggaa
     1981 tgaggggttt cggaacggtc agcgtatgcg gccgcatccg tttgcccata ccggccatgg
     2041 ggacgatgat cttcatttgt gggttgaatg ttccgggttt cgggtttcgg gttccgggtt
     2101 ttgcatgccc gcgtttcctt cccgattcta tcgctacccg atttattgat tgaatttaaa
     2161 cttgaatgat ttcgtcaaat gcaccattca ggtggccttg ccttattttc ctgttgaacc
     2221 gaaaccaccg gtgcctcgat cggtgaggga gagttcttcg gcatcggtcc attccacacg
     2281 ttcgtggcgg gcgacgacca gttgcgcgac gcgttcgccg tcttcgatgc ggaaggtttc
     2341 gttggacagg ttcaccagga tcaccttgac ttcgccgcga tagtcggcgt cgatcgtgcc
     2401 gggggagttg agtaccgtaa cgccacgttt cgcggcgaga ccgctgcgcg ggcgcacctg
     2461 cgcttcataa ccaaccggaa gttcgaggaa caagcccgtg ggtacgagcg cgcgctcgag
     2521 gggtttcaac tcgattggag tttcgaggaa ggcgcgaagg tccatgccgg cggcgtgggc
     2581 cgtttcgtaa gcaggtagcg ggtgtcggga acgattgatg atcttgacgt tcatcgtgta
     2641 gagtttcgat ggttttaaaa atgaaatgac ggtagaaaat ccgcgtatga catttcaaat
     2701 ggcattttcg cgaattgtta ccgactccat agtctcgaca aaggtagctt tttccgatgc
     2761 tcataggccg ctgcaacacc tatccaggtc aggagtaagc cggagttgat cagcgtagtt
     2821 tgccaggtag aaccgctgaa tccgcgaagc cattcgctga ggaaaaagat
//
LOCUS       DAOTON010000316         2868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27204_length_2868_cov_4.035905, whole genome shotgun sequence.
ACCESSION   DAOTON010000316 DAOTON010000000
VERSION     DAOTON010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27204_length_2868_cov_4.035905"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..972
                     /locus_tag="P5292_07020"
     CDS             166..972
                     /locus_tag="P5292_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRS38916.1"
                     /translation="MLLDPIPADTVHAAVGFVKYVSGAIKSIEDHYAVYTGVGMAILI
                     WYKRIRQAYQWVRKQLSDLNKWFLRRFREYVIKHVIKMDPLELHKTISEGFSYINSAL
                     TAHREKHDEQYSDLLKVLNNQDQLLQRVAKETLPNGGEHSISDKLDRILIASKIQWDS
                     SPHGEFETGRDGRYKFVSSAYCDITGRSREDLLGWGFISAIHEPEQSQIRELIEEGVK
                     DRRKFDVQYTIQRRDGSLVKVTHYWQPMFRNLDIEDEFYGFMGRIVTLES"
     gene            1000..1509
                     /locus_tag="P5292_07025"
     CDS             1000..1509
                     /locus_tag="P5292_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38917.1"
                     /translation="MKSFIQYLEGLANKTQAVFFILALYHSGLYLSLVPASWMPYVDF
                     ACVLALATFKAIAPTGVLTKGQTLFFWVSTGLGLIITFGQAFLETALFNINPELIGKV
                     ITFATGLLAFWQYINGVNLSNKVAVLKLEQKLSPDVMAPDALPTLSDKIRAKYVIPKA
                     VIRKYKLAA"
     gene            1509..2006
                     /locus_tag="P5292_07030"
     CDS             1509..2006
                     /locus_tag="P5292_07030"
                     /inference="COORDINATES: protein motif:HMM:NF040141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5675 family protein"
                     /protein_id="HRS38918.1"
                     /translation="MELLLTRNTFTPKSTIGNLTDLDGRLLTAVSAKPIYILEDRDRG
                     LNASMTLSEIASKKVHAQTAIPAGRYEIIISFSNRFKRPLPLLLDVPGYAGIRIHNGS
                     FPEHTEGCLLPGLVKGVDSVTQSRDFFNKKLFPWIESVLKKEKMFITITRDAAEWNYF
                     QVRAA"
     gene            2003..2620
                     /locus_tag="P5292_07035"
     CDS             2003..2620
                     /locus_tag="P5292_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38919.1"
                     /translation="MKINANTILILIGLAILAYLIWMRPAPPSVTTVYRDTSRTPAVV
                     ITMPQPTIQIPGPAQGPQINVNGLDSATLRTMLVDLLEQHRRLYAEHSTYRQYDTVLA
                     DSNAVESLSVAVQHNRLQRLTRKLDVINTRTIEQAAAPWIYAGVYALADSSGIPSFGP
                     TISVALRSGAVIRAGKSLQGNQFMLGADFPILKKKWKHRARDTAD"
     gene            complement(2675..>2868)
                     /locus_tag="P5292_07040"
     CDS             complement(2675..>2868)
                     /locus_tag="P5292_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38920.1"
                     /translation="LKQRDVRYGGKNKTSALLQADTEKEFLRNTWLMLTAVGNACIVA
                     FLAIQALVILKAIANQSLP"
BASE COUNT          709 a          761 c          705 g          693 t
ORIGIN      
        1 atgcctttac ttatttccgg acaaacccct ggacaaagcc gctgcaagac tttctgacgt
       61 actataacgc tactgcttcc cagatcacaa aagacacgct gatcatcacc gatatggcct
      121 tgtttggagc caagtgggcc atgaaagcca agccttgaat gaattatgct gttagatccg
      181 ataccagccg acaccgttca tgcagcggtc ggatttgtga agtatgtttc cggagcaatc
      241 aagtccatcg aagaccacta tgctgtttac accggagtcg gaatggctat tctgatatgg
      301 tacaagcgaa ttcgacaggc gtatcagtgg gtcaggaagc agttgtcgga tctgaacaaa
      361 tggtttcttc ggcggtttcg tgaatatgtc ataaaacatg tgatcaaaat ggatcctctt
      421 gagttacaca aaaccatctc cgaagggttc tcttacatca acagcgctct gactgctcat
      481 cgtgaaaaac atgatgagca gtattcagac ttgctcaagg ttctgaacaa tcaagatcag
      541 ttgcttcagc gtgtggccaa agagaccctg ccgaacggcg gggagcatag catcagcgac
      601 aagcttgatc ggatcctaat tgccagcaaa attcaatggg atagttcgcc gcatggcgaa
      661 tttgagaccg gccgcgacgg tcggtataaa ttcgtgagtt ctgcatactg cgatatcacg
      721 ggccggtccc gcgaagatct tcttggttgg ggctttattt cagctataca cgagcccgaa
      781 caatcgcaga ttcgtgagtt gattgaagaa ggggtaaaag atcgccgaaa gttcgatgtg
      841 caatacacga ttcagcggcg agacggatcc ttagtaaaag tcactcatta ctggcaacca
      901 atgtttcgca acctcgacat cgaagacgaa ttttatggat ttatgggcag aattgttacc
      961 ttggaatcct aaacctaaac ctcaaaatca cacactacca tgaaaagttt cattcagtat
     1021 ctggagggcc tggcaaacaa aacccaggcc gtgttcttca tcttggctct gtatcattcc
     1081 ggcctgtacc tgagcctggt gccggcatcg tggatgcctt acgtggactt cgcttgcgtg
     1141 ttggcactgg caacgttcaa agccatcgct ccgaccggag ttcttaccaa aggtcaaacg
     1201 ctctttttct gggtgtccac cggactgggc ctgatcatca ccttcggtca agccttcctg
     1261 gagacagcgt tattcaacat caatccggaa ctgatcggaa aggtaattac gttcgccacc
     1321 gggcttcttg ctttctggca gtatatcaat ggcgtaaacc tcagcaacaa agtcgcggtg
     1381 ctgaaactgg aacagaagct atcgccagac gttatggctc cggacgcctt gcccactctt
     1441 tccgataaga tccgggcaaa gtatgtaatc cccaaggctg tcattcgcaa gtacaaactg
     1501 gctgcgtaat ggaactgctg ctcacccgca atactttcac ccccaagtcg accatcggga
     1561 acctcacgga tctcgatggt cgcttgctta ccgctgtgtc ggctaagcca atctacatct
     1621 tggaggaccg cgaccggggg ctgaatgctt ccatgaccct gtccgagatt gcttccaaga
     1681 aagtgcatgc acagaccgcc atccccgccg gccggtacga gatcatcatc agcttttcga
     1741 accggttcaa gcgacccctg cccctgctct tggacgtgcc tggatatgcc ggcatccgga
     1801 ttcacaacgg ctctttcccg gagcatactg aagggtgcct gttgccgggg ctggtcaagg
     1861 gagttgattc agtgacccaa tcccgcgatt ttttcaataa aaagctgttt ccgtggattg
     1921 agtcggtact gaaaaaagaa aaaatgttca tcaccattac gcgggatgct gccgaatgga
     1981 actattttca ggtccgtgct gcatgaagat caacgccaat acgatactga tcctgattgg
     2041 attggcgatt ttagcctatc ttatctggat gcggccggcg ccgccttcgg ttaccacggt
     2101 gtaccgggat acttccagga ccccggcggt ggtgatcacc atgccgcagc ccacgattca
     2161 gatcccgggc ccggcccagg ggccgcaaat caatgtcaac ggccttgata gcgccaccct
     2221 gcgaaccatg cttgtagacc tcctggaaca gcaccggagg ctgtatgccg aacattcgac
     2281 ctaccggcag tacgataccg tcctggccga ctccaatgcc gtagaaagcc tttccgtagc
     2341 ggtacagcat aataggctgc agcggctcac gcgcaagctt gacgtcatca atacccgaac
     2401 cattgaacag gccgctgcgc cttggatcta tgcgggcgtg tacgccctgg cagacagtag
     2461 tggcatcccg tcatttggcc ctactatatc cgttgcactt cgatcaggcg ccgtgatcag
     2521 ggctggaaag tccctgcagg gcaaccaatt catgttgggc gccgactttc ccatactgaa
     2581 aaagaaatgg aagcaccgcg cacgagacac cgcagattga atggcaggat aaaaaaaggc
     2641 ccccttttca gggggctttt tcattgcatg atgtctacgg tagcgattgg tttgcgattg
     2701 ctttcaatat aacaagtgcc tgaattgcta aaaatgcaac aatacacgcg tttccaacgg
     2761 ccgtcaacat taaccatgta ttccgtagga attctttttc cgtatccgct tgcagtaaag
     2821 ctgaggtttt gtttttgcca ccatagcgga catctctttg cttcagct
//
LOCUS       DAOTON010000317         2868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27213_length_2868_cov_2.023107, whole genome shotgun sequence.
ACCESSION   DAOTON010000317 DAOTON010000000
VERSION     DAOTON010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27213_length_2868_cov_2.023107"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="P5292_07045"
     CDS             <1..559
                     /locus_tag="P5292_07045"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS38921.1"
                     /translation="VPAFHPHDEVAALPGRGDFALYHGNLSIGENNRAAVWLAEEVFA
                     NPGLQLVIAGSRPSAELKKIINDKPHIRLVADPDPATIYRLVREAQVNVLPTFQSTGI
                     KLKLLAALFTGRHCLVNTPMVSGTGLEQLCVIKDDPDDFRNAVAGLLQQDYAATQQEL
                     RKSVLDRDFSNRKNAGKLAGMIFID"
     gene            complement(564..1244)
                     /locus_tag="P5292_07050"
     CDS             complement(564..1244)
                     /locus_tag="P5292_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS38922.1"
                     /translation="MSVVRLSNAEIFQGNHLVLSRVNLEIGKGEFVYLIGKTGSGKSS
                     LLKLLYGDLSLTQGEGSVAGFSLPQLTTSQVPYLRRKLGIVFQDFQLLTDRSVNANLL
                     FVLQATGWSDKAKMQSRIQDVLEKVGLGTKGFKMPHELSGGEQQRVVIARAMLNDPEL
                     ILADEPTGNLDPETSEGIMRLLFDISRTGTAVMMATHNYLLIEKFPSRILKCEQGIVL
                     DSSVSEPA"
     gene            complement(1340..1543)
                     /locus_tag="P5292_07055"
     CDS             complement(1340..1543)
                     /locus_tag="P5292_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRS38923.1"
                     /translation="MYILKVKGTAKIPDYVQLRDENFTLLAYFRVDRPEKALSKAGLG
                     NREAELIELIRNMPYGKIQKLDD"
     gene            complement(1610..2464)
                     /locus_tag="P5292_07060"
     CDS             complement(1610..2464)
                     /locus_tag="P5292_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38924.1"
                     /translation="MKTIRIAAVVLAVFASITACKKDEKETLDFDYQSAVDNSLAEGI
                     YSDVNNISNQAVENGNTGLATYRTGEGNGLLSVCATVTVTPDSAGNGGSLVVDFGSTN
                     CLCRDYRYRRGRVLVDYTGRYRDSASVITTTFQDYYVGTDSSNMYQVLGSKTVTNKGR
                     NGSGNIWFTVEVDGQLRNSNNQTMEWNSSRTRTWTAGESTPLDWQDDEYLIGGTADGI
                     SFEGRPYTLNITTPLHIDFDCRWIRAGVIELTPQGKSARILDYGPDNCDAAATVTVNG
                     TTFNITLR"
     gene            2654..>2868
                     /locus_tag="P5292_07065"
     CDS             2654..>2868
                     /locus_tag="P5292_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38925.1"
                     /translation="MTLRRWFVLAFLLILQPAIAQKTAIFLDPDRNYREGLDLFGKQK
                     YGAAREYFEAVLKNPKGSQESRMGSAY"
BASE COUNT          636 a          783 c          773 g          676 t
ORIGIN      
        1 tgtgcccgcg tttcatccac acgacgaagt ggctgcgctg cccgggcgtg gcgacttcgc
       61 gctgtatcat ggtaacctga gcatcggcga gaacaaccgg gccgcggtat ggctcgcgga
      121 agaagttttc gcaaatcccg gcctccaatt ggtaatcgcc ggaagccgtc cttcagcgga
      181 attgaaaaag ataatcaacg ataagccgca catccgactg gtggccgacc ccgatccggc
      241 tacgatctat cggcttgtcc gggaggccca ggtcaacgtc ctgcctactt ttcagtcgac
      301 gggcatcaag ctcaaattgc tcgccgcact atttaccggt cgtcattgtc tcgtcaacac
      361 acccatggtc agcggaacag gcctggaaca attgtgtgtg ataaaagatg atccggacga
      421 tttccggaat gcggtagccg gcctactgca acaggactac gcggctaccc aacaggaatt
      481 gcggaagtcg gtcttggatc gcgatttctc caaccgtaaa aatgccggta aactagccgg
      541 catgatcttc attgattgac gtgttaagca ggctcgctca cgctggagtc gagcacgatg
      601 ccctgttcgc acttgaggat gcgggaaggg aacttttcga tcagcaggta attgtgggtt
      661 gccatcatca ccgcggtgcc ggtcctgctg atatcgaata acaaccgcat gatcccttcc
      721 gaggtctccg ggtcaagatt accggtcggt tcgtcggcca ggatcagttc cggatcgttc
      781 aacatggcac gcgcgatcac cacccgctgc tgttcaccac cgctgagttc gtgcggcatc
      841 ttgaaaccct tggtgccaag cccgactttt tccaggacat cctgaatcct gctctgcatc
      901 ttcgccttgt cgctccaacc ggttgcctgc aacacgaaca acaggttggc attgacggag
      961 cggtcggtga gtagctggaa gtcctgaaat acgattccga gtttgcggcg gaggtaggga
     1021 acctgcgatg tggtcagctg cggaagggag aatcccgcga cggaaccttc accctgcgta
     1081 agggaaaggt caccgtaaag caacttcaac agcgagcttt ttccgctgcc ggtcttgccg
     1141 atcaggtaaa cgaattcgcc tttgccgatc tccaggttga cacgtgaaag cacaagatgg
     1201 tttccctgaa aaatttctgc attggaaagt cggacgacac tcatggctga cggtgcttat
     1261 ggtgcaaagg taggaggaag ttaaccggat tggccggtgg ttaagcccac ctgctaacag
     1321 gcttccgtgt aatgcgccat cagtcgtcca gtttctggat ctttccgtag ggcatgttcc
     1381 ggataagttc gatcaactcc gcttcccggt taccaagtcc tgctttcgac aaggcttttt
     1441 cagggcgatc cacccggaag taagccagca aagtgaaatt ttcgtcccgc aactgtacgt
     1501 aatcggggat cttggcggtg cctttcactt tcaaaatgta catcacggca aagctaacaa
     1561 aaaagcgggc ctgccggttt tggcaagccc gcgctaaatt tttggagtat cagcgaaggg
     1621 tgatgttgaa cgtcgtgccg ttgaccgtaa cggtcgcggc cgcatcgcag ttgtccgggc
     1681 catagtcgag gatacgggcc gatttaccct gcggggtcag ttcgatcacg ccggcacgga
     1741 tccagcgaca atcgaaatcg atgtgcaaag gggtggtgat gttcagcgta tagggacgac
     1801 cttcgaacga tatcccgtcg gcagttccgc cgatgagata ttcatcatcc tgccagtcga
     1861 gcggagtgga ttcccccgcc gtccaggtgc gcgtccgact cgagttccat tccatcgttt
     1921 gattgttgct gttcctgagc tgaccgtcga cttcaacggt aaaccagatg ttgccggaac
     1981 cgtttcggcc tttgttggtc accgtcttgc tgcccaggac ctggtacata ttgcttgaat
     2041 cggtaccgac ataataatcc tggaaggtcg tggtgatgac cgaggcggaa tcgcgataac
     2101 gtccggtata atcgaccagt acgcggccac gacggtaacg ataatcacgg caaagacaat
     2161 tggtacttcc gaaatccacc accaggctgc cgccgttacc tgcactgtcg ggtgtgacgg
     2221 ttaccgttgc acacacactc agcaggccgt tgccttcacc ggtgcgatag gtcgccaggc
     2281 cggtattgcc gttctcgact gcctggttgg agatgttatt gacgtcggag taaatacctt
     2341 ctgcaaggct gttgtcgacc gccgactgat agtcgaagtc gagcgtttct ttctcatcct
     2401 tcttacaggc tgtaatgctg gcaaacacgg ccagaacaac cgctgcgatg cgaatcgttt
     2461 tcatgttttt cgatttaccg ggacaccttt gactgcaaag tattaccagg gtttagcctt
     2521 aaacggtcga gtcccggatt tgttgccgtg tttaaaactg tgttggtaat aacaactcct
     2581 tcaaaacgaa ctaactgggg ctccgccaat aactttgctc gttacagcgt ttcacccgaa
     2641 atccgcccca agtatgacct tacgacgttg gttcgtgttg gcgtttttgc tgatcctaca
     2701 gcccgcaatt gcccagaaaa ccgcaatctt ccttgacccc gaccggaact accgggaagg
     2761 actcgatctg ttcggtaaac agaagtacgg cgccgcccgt gagtatttcg aagccgtttt
     2821 gaaaaacccc aagggctcgc aagagtcccg catgggttcg gcctatta
//
LOCUS       DAOTON010000318         2866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27237_length_2866_cov_2.847385, whole genome shotgun sequence.
ACCESSION   DAOTON010000318 DAOTON010000000
VERSION     DAOTON010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27237_length_2866_cov_2.847385"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="P5292_07070"
     CDS             complement(<1..240)
                     /locus_tag="P5292_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HRS38926.1"
                     /translation="MEFTAKQICELLNGTLEGDPGIKVNQLSKIEEGVPGSLTFLANP
                     KYESFLYTTKASLAIVNQDFRPVQPVIPALLRVPDA"
     gene            complement(384..1604)
                     /locus_tag="P5292_07075"
     CDS             complement(384..1604)
                     /locus_tag="P5292_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRS38927.1"
                     /translation="MANQLKIVNDPIYGFIRLPSERVFLLIEHPYFQRLRRIRQLGMT
                     HLVYPGALHTRFHHALGAMHLMGEAVEVLRSKGHLITPEEAEAVTIAILLHDIGHGPF
                     SHALEDSIVPGIRHEELSSLFMDRLNRDFDGSLQLALTIFRDQYPKRFLHQLVSSQLD
                     MDRLDYLSRDSFFTGVSEGVISHDRIIKMLEVQDDQLAIEEKGIYSIEKFIVARQSMY
                     WQVYLHKTVVGAERLLISILKRAGELVRSGQHLFATPALDLFLRNSFTLDDFRERPDL
                     LDAFAELDDSDITTAVKIWSKGPDYILRELCFRLVNRRLFKIVLRKDPFSESEIREIS
                     ERIRDRFSVDERDMHYFLETGSIVNNAYNSSSDKINIILKDGTVKDIAEAADTLNIKE
                     LSTTVEKHYLLFPK"
     gene            1824..>2866
                     /locus_tag="P5292_07080"
     CDS             1824..>2866
                     /locus_tag="P5292_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HRS38928.1"
                     /translation="MDKIRILWADDEIDLLKPHLLFLQEKGYDVTTVTNGEDALEAVR
                     KSDFAIVFLDENMPGLSGLETLSRLKNIRADVPVIMITKSEEEHIMEQAIGSKIADYL
                     IKPVNPNQILLALKKNLENKRLISEKTTSDYMQDFRQIGMSLSDKLNWQEWQEVYRKL
                     VFWELELEKSSDQSMYEILTSQKNEANNLFSRFVERNYLQWLRTPDEQATPVLSHQLM
                     KKRILPAVDASADPVFLILIDNLRYDQWKTIQPILADLYRVQDDELYCGILPTATQYA
                     RNAIFAGLMPSEIESRFPQWWKNDEDEGGKNMHEADFLGDTLKRFRKEYKFSYTKITN
                     HNEGKELVESLSN"
BASE COUNT          760 a          761 c          705 g          640 t
ORIGIN      
        1 ggcatccggt acgcgcaaaa gggcagggat aaccggttgt acgggtcgaa aatcctgatt
       61 gacgatcgcc agtgaagcct tcgtggtgta gaggaaggat tcgtacttcg ggttggctaa
      121 aaaagtaaga gaacccggaa caccttcctc gatcttgctc agctgattta ccttgatgcc
      181 gggatcacct tccagcgtcc cgttcaacaa ctcacagatc tgttttgcgg taaattccat
      241 ggtccgaatt gaggttctaa ggtacggcta aagaacaata gcagtcaatt gcagtttagc
      301 taaccaccta aaaacgagcg aaaaacaggg atattggcgt gaaaaaataa aatgacgatg
      361 aaccgacggg aaaggctatc ggatcacttc gggaacaaga ggtaatgctt ttccacggtt
      421 gtgctgagtt ccttaatatt cagggtgtcc gccgcttccg caatatcttt cacggtaccg
      481 tccttcagga tgatattgat cttatcgctg ctggaattat aggcattgtt gacgatgctg
      541 ccggtctcca gaaaatagtg catatcccgc tcatcgactg aaaaccgatc ccggatccgt
      601 tcactgattt cccggatttc agactcggag aatggatcct ttctcaatac gatcttgaac
      661 agtcgccggt tgaccagtcg gaaacacaat tcacgcagaa tgtaatccgg gcctttgctc
      721 cagatcttga cagctgttgt gatatcactg tcatccaact ccgcgaaagc atccagcaag
      781 tcgggacgtt cgcggaaatc atccagcgta aacgaatttc gcagaaagag atccaaggcg
      841 ggtgtggcga acaaatgctg cccggagcgc accaattccc ctgcccgttt cagaatactg
      901 atcagcaatc tttccgctcc tactacggtc ttgtgtagat aaacctgcca gtacatcgac
      961 tgacgggcaa cgatgaactt ttcgatgctg tagatgccct tctcttcaat ggccagttgg
     1021 tcgtcttgca cttccagcat cttgatgatt cggtcatggc tgataacccc ctcgcttaca
     1081 ccggtaaaaa aactatccct gctcaggtaa tcgaggcgat ccatatcaag ttgactggat
     1141 accaattggt gcagaaatcg tttgggatat tgatctctga aaatggtaag cgccaattgc
     1201 agcgatccgt cgaaatcccg gttcaggcga tccatgaaca aggaggacaa ttcctcatgc
     1261 cggatccccg gaacgatgct gtcttcgagc gcatgtgaaa acggaccgtg cccgatatcg
     1321 tggagcagta tcgcgatggt gacagcctcc gcctcctctg gcgtaatgag atgccctttc
     1381 gaccgaagta cctcgacagc ctctcccatc aggtgcatgg cgcccagcgc atgatggaac
     1441 cgtgtatgca atgcgccagg ataaaccagg tgcgtcatgc caagttgccg gatccggcgc
     1501 aaccgctgga aatatggatg ctcgatcaac aggaacaccc gctcactggg gagccgaatg
     1561 aatccataga tcgggtcatt gacgattttt aactggttgg ccacaggcta ttgcatggtt
     1621 cttgcgtgaa ccgcggcaaa tatcggtctt tccacgggat tcatttctct cttcggattc
     1681 aattaaagcg gaaacctgcc gttgatgagc agtcggaaag ttttccggac gattccaaac
     1741 cgagataata ctgaaatccc ctcatgagac ccacagttgc tacctttgac gcaggcacca
     1801 ccgccccctt cctcccgctg aatatggaca agatccgaat actctgggcc gacgatgaaa
     1861 tcgacctcct gaaaccccac ctcctcttcc ttcaggaaaa aggctacgat gttaccacag
     1921 taaccaacgg cgaggatgca ttggaggcgg tcaggaagag cgatttcgcg atcgtatttc
     1981 tggatgagaa catgcccggt ctgtcaggcc tcgaaaccct ttcccgactc aagaacatcc
     2041 gggcggacgt accggtaatc atgatcacga aaagcgaaga agagcacatc atggaacagg
     2101 cgatcggcag caagatcgcc gactacctga tcaaaccggt caatcccaac cagatcctgc
     2161 ttgcactgaa gaagaacctg gaaaacaaac ggctgatcag cgaaaagacc acctccgact
     2221 acatgcagga ctttcgccag atcggtatgt ccttgagcga taaactgaat tggcaggaat
     2281 ggcaggaggt ctaccggaaa ctcgtattct gggaacttga gttggaaaaa tcctcggatc
     2341 agagcatgta cgagattctg acctctcaga agaatgaagc caacaacctt ttctcccgct
     2401 tcgtcgagcg caattacctg caatggttac gtacgccgga cgagcaggct acgccggtgc
     2461 tttcgcacca attgatgaag aagcggatcc tccctgcggt ggacgcttcg gctgatccgg
     2521 tcttcctgat cctgatcgac aacctgcggt acgatcaatg gaaaacgata caaccgatcc
     2581 tggcggatct ctaccgcgtt caggatgacg aactgtactg cggtatcctg cccaccgcca
     2641 cccagtatgc ccgaaacgcc atcttcgccg gtttgatgcc ttcagaaatc gagtcccggt
     2701 tcccacaatg gtggaagaac gacgaagacg agggcggcaa gaacatgcac gaagcggatt
     2761 tccttggcga cacactgaaa agattccgca aagagtacaa gttctcgtat accaagatca
     2821 ccaatcacaa cgaaggcaag gaattggtcg agtcgctctc caataa
//
LOCUS       DAOTON010000319         2862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27286_length_2862_cov_4.231920, whole genome shotgun sequence.
ACCESSION   DAOTON010000319 DAOTON010000000
VERSION     DAOTON010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27286_length_2862_cov_4.231920"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..916
                     /gene="pyrF"
                     /locus_tag="P5292_07085"
     CDS             92..916
                     /gene="pyrF"
                     /locus_tag="P5292_07085"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRS38929.1"
                     /translation="MTRQELTRLIRAKRSYLCIGLDTDIRKLPKHLLKAEDPVFEFNR
                     QIIEATADLCVAYKPNIAFYEAEGPSGWVSLERTLAAIPKDCFTIADAKRGDIGNTSS
                     LYARTFFERYAFDSVTVAPYMGKDSVTPFLEFPGKWVILLGLTSNAGSLDFQMLDVAL
                     PGHAAGEQLFEHVLHTSQRWSGPDSMMYVIGATHPELFTRVRKIVPDHFLLVPGVGAQ
                     GGDLEAISKAGMNDQVGLLVNSSRQIIYASSGEDFAEAARKEAMALQQEMSRLLDA"
     gene            977..2275
                     /locus_tag="P5292_07090"
     CDS             977..2275
                     /locus_tag="P5292_07090"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HRS38930.1"
                     /translation="MNERFLHHVWSQRVFQFEPLLTKSGEPLQILQTGLYNHDAGPDF
                     FNARLRIGGAEWAGNVEIHLRSSDWNRHGHQSDPLYGNCILHAVAVHDAEVFDVHGRM
                     LPTFVFPESALAAWWGHYELLVQRPSAIACTDRLPFVPPHLWSSWIDRMLMERLEERV
                     RRVDQQLHVSRGDWEDAIFRAVSRAMGFAVNADPMERVAASLPLKVLLKTAQRRDSLE
                     ALLFGQSGLLQAPAPDEYSSGLLEEYRFQRHKLGIEPIPLPGWKFLRMRPRNFPTIRL
                     AQLASLIQTAFPLLQRCLEEHELKSLLSRFRVDVSPYWHNHYRFGVPTKFEEKWLGDD
                     AIRILLLNTVVPFVFARGKAEGNSYWQEKALRWLCELPSEKSLPLREWRTAGVKPQHA
                     GDGQALLHLLRNYCTPKKCLSCSIGNHLIHSSDEALRIDP"
     gene            2250..2477
                     /locus_tag="P5292_07095"
     CDS             2250..2477
                     /locus_tag="P5292_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRS38931.1"
                     /translation="MKLFESIRDRWERQAFGVCSWLGERLNMQTASIRLFFIYASFLA
                     VGSPVILYMILAFWIKMKNYVFNRRTSVWDL"
     gene            2521..>2862
                     /locus_tag="P5292_07100"
     CDS             2521..>2862
                     /locus_tag="P5292_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38932.1"
                     /translation="MLITLHLLLELRAQAQDWRALGPSTFPEFPGKSDFFLKNARGIG
                     RAGAVRFLKQADGALIRILLTPYGGMWKWNQEMLIWEVITGSDLLEGGVADLQQHPKN
                     LSKWYAVAGDAD"
BASE COUNT          613 a          746 c          796 g          707 t
ORIGIN      
        1 aagcgttgga aaggtaagcg gtttttcgat tggatgaatt cgctgcacgg tttcgacaat
       61 acatcccgcc ggcattccgt acttttacgg cgtgacacgc caggaactga cccgattgat
      121 tcgcgccaag cgatcctacc tgtgcatcgg gctcgatacg gatatccgca aactgccgaa
      181 gcatttgcta aaggcggagg atcccgtatt cgaattcaac cgtcaaatca ttgaagcgac
      241 cgctgacttg tgtgtcgcct ataagcccaa catcgctttc tacgaagcgg aaggtccgtc
      301 cggatgggtg agtctggagc gtacgcttgc cgcgattccc aaagattgtt tcaccatagc
      361 ggatgctaaa cgaggtgata tcgggaatac ctccagctta tatgcccgga catttttcga
      421 acgctatgct ttcgattcag tcaccgttgc accctacatg ggcaaagatt cagtcactcc
      481 gtttctggag tttccgggga agtgggtgat cctgcttgga ctgacgagca atgccggaag
      541 cctggatttc cagatgctcg atgtcgcctt gcccggacac gcggccggag aacaactttt
      601 cgaacacgtc ctgcatacat cccaacgctg gtccggaccc gattccatga tgtacgtgat
      661 cggtgcgaca catccggagt tgtttacacg tgttcgtaag atcgtaccgg atcattttct
      721 ccttgttccc ggagtgggcg cacaaggagg cgacctcgag gccatttcga aagcgggcat
      781 gaacgaccag gtgggtttgc tcgtgaacag ttccaggcaa atcatctacg cttcctctgg
      841 cgaagacttc gccgaagcgg caagaaaaga ggcaatggca ttgcaacagg agatgtcccg
      901 attgctcgac gcttgagttt tccgcattta ttgagagtga attaatgttc tgccgatttg
      961 ctttgcagtc ggtcccatga acgaacgatt cctccaccac gtctggtcac aacgcgtttt
     1021 ccagttcgag ccactgctaa ccaaatcggg cgagcccttg cagattctgc agacaggcct
     1081 ctacaatcat gatgcaggtc ccgatttctt caatgcccgt ttacgtatcg gtggggccga
     1141 atgggcggga aacgtagaga ttcacctgcg ttcctccgac tggaatcgcc atggtcacca
     1201 atccgacccg ttatacggca attgcattct gcatgccgtt gccgtgcacg atgcggaggt
     1261 gtttgatgtg catggccgaa tgctgccgac attcgtattc ccggaatcag cgttggctgc
     1321 ctggtgggga cactatgaac tactggtgca acggccgtca gctatcgcgt gcaccgaccg
     1381 gctcccattc gtgcccccgc acctttggag ttcctggatc gatcgcatgt tgatggagcg
     1441 actcgaagag cgtgtcagaa gagtcgatca acagttgcat gtttcacggg gcgactggga
     1501 agacgcgatc tttcgtgcgg tcagccgcgc gatgggtttt gctgtcaatg ccgatccgat
     1561 ggaacgtgtt gcggcttccc tgccgctgaa agttctcctt aaaacagcac aacggaggga
     1621 ttcgttggaa gctttgctct tcggacaatc cggcttgttg caggcgccgg ctccggatga
     1681 gtatagctca ggtttgctgg aggagtatcg attccagcgg cataagctgg gaattgagcc
     1741 gataccactg cccggatgga aatttcttcg gatgcgaccg cgaaatttcc ccacgattcg
     1801 gctggcgcaa ctcgcatccc tgattcaaac cgcttttccc ttgctccagc gttgcctaga
     1861 agaacatgaa ctcaagtcac tcttatcgag gtttcgggta gacgtttcgc cctattggca
     1921 caatcactac cggttcgggg tgccaaccaa atttgaggaa aagtggctgg gcgatgatgc
     1981 aatacggatt ttgttgttga acacagtcgt tcctttcgtc tttgccagag gaaaagctga
     2041 gggcaattca tactggcagg agaaggcgct gcgctggctc tgcgaattgc cttctgaaaa
     2101 aagcctgccg ctgcgcgaat ggaggacagc cggagtaaag ccgcagcatg caggcgacgg
     2161 acaggccttg ttgcacctgc ttcggaacta ctgtacgcct aaaaaatgtt tatcttgcag
     2221 catcggcaac cacttgatcc attcttccga tgaagctctt cgaatcgatc cgtgaccgtt
     2281 gggaacgtca ggcattcggc gtatgttcct ggttgggcga gcggctgaat atgcagaccg
     2341 catcgatccg gttgtttttt atctacgcca gttttctggc ggtcggctct ccggtcatct
     2401 tatacatgat tctagctttc tggatcaaaa tgaagaacta tgtcttcaac aggcgtacct
     2461 cggtctggga tctttgaaag cttccggcag ccggtcggcg tctccaggtg cctgaccgtt
     2521 gtgctgatta cccttcactt gcttcttgaa cttcgggccc aggcacagga ttggcgcgcg
     2581 ctgggacctt ccacgtttcc ggaattcccc ggtaagtcgg atttctttct gaagaatgcg
     2641 cgcggcatcg gcagggcggg agcagttcgg ttcctgaagc aagccgatgg agcgctgata
     2701 cggatcctgc tcacaccgta tggcggtatg tggaaatgga atcaggaaat gttgatctgg
     2761 gaagtgatca ccggctctga tttgctggag ggcggtgtgg ccgatttgca acagcatcct
     2821 aagaatttgt caaagtggta cgcggtcgca ggcgatgctg at
//
LOCUS       DAOTON010000320         2860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27310_length_2860_cov_3.182175, whole genome shotgun sequence.
ACCESSION   DAOTON010000320 DAOTON010000000
VERSION     DAOTON010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27310_length_2860_cov_3.182175"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2860)
                     /locus_tag="P5292_07105"
     CDS             complement(<1..>2860)
                     /locus_tag="P5292_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38933.1"
                     /translation="GCTPNRLDNVFFDANSLGAGDTVYLPHLEQYAGDLTVNGVTGSP
                     TFFGDYITLYGSLDLNHQMNWRLYQTHLRSPRTGSILRTTGTPFMYLTYGDSCWYTLQ
                     DSLYAEWIYQQNGNVDYNGQNVNTRLLSGYPAAQAITANSTFYLKDMYFDGTFIGSGT
                     TDIIFDKGPAYCRNNRASQFRNMYFIERGFLSDSVTVASLVADQALDIGNANTIGFAQ
                     FYHDVTVSGSNSIDDLRLSNGGFNYSQAPGETLTLGTNLQVQADCNGLAGIRSTVSGT
                     PTNISMTSGSITVDYVSLNSVAAGGGASFTANNSVDGGNNTGWTINSPAPRTVYWVGG
                     SGNWSDPTHWSLSTGGASGACVPAAIDQVIFDGNSGLASGILTLDVTNVSAAGIDFSA
                     AGSDITVNGTTISVTGDLALAPVMNWNVATTNLLPGASGIDLTCNATELDSVTINGTG
                     TVTLLDACAGIQLQLLNGNLDADGNDITMSRMTSNLGTDVNFGPMTLTVSYFYLQGSL
                     SNSRQADVVMRWLAPVYSSLGYDFRTTGAFRNITVLDIGARFFLNSFVCEQFTAYQYV
                     NVYSQLASDSIRKAIFFDDFAMSGNIVCDTIFLNNPGKTSNLGGNINFSELVCNGTPA
                     FPVFLKGNPIALLNKTSGQVCIANALLQDVTVGGGATFNAGNGCVDLGGNTGWTYAPC
                     TVVSNVWPGDANYDLVCNNSDILDIGVAFGQTGPVRAGATLSWTAQAATDFNGWFVSA
                     VNFKHADCDGDGTVGFADTLAVNQNYGQTHPARLQPTPAQIDAALPPLVIDATPDTVA
                     PGATVNVAIQLGTITQAVDSLYGIAFTVYYDPTVVDTNSLVTDFSTSWLGTAGVDLIT
                     FYRHTPSAGRIDFALVRNDGQNVFGGFGDIALFDVVIVDNISTLTDALFTPGNLRAIT
                     ASQFPLLFGSQNDRVVLDPTLTRVPVPVLSTHLVVFPNP"
BASE COUNT          608 a          775 c          879 g          598 t
ORIGIN      
        1 ccgggttcgg gaagaccacc aagtgggtgg agaggaccgg aaccggaacg cgcgtcaggg
       61 tcggatccag cacgacacgg tcgttctggc tgccgaacaa gagcgggaac tgcgaggcgg
      121 tgatggcccg gagattaccc ggcgtgaaca aggcgtcggt cagcgtagaa atgttgtcga
      181 cgatgacgac atcaaacaag gcaatgtcgc cgaagccgcc gaacacattc tgtccgtcgt
      241 tgcgcaccag cgcgaagtcg atacggccgg ccgagggcgt gtggcgatag aaggtgatga
      301 gatcgacacc cgccgtaccg agccagctcg tgctgaagtc ggtcaccagc gaattggtat
      361 ccaccacggt cggatcataa tagacggtga aggcgatgcc gtacagactg tcgacggcct
      421 gtgtgatcgt gcccaactga atggccacgt tcaccgtggc gcccggagcc accgtatccg
      481 gcgtagcgtc gatcaccagc ggaggcaggg ccgcgtcaat ctgcgccggt gtcggctgta
      541 gccgcgccgg atgggtttgt ccgtagttct ggttcaccgc cagtgtatcg gcgaatccga
      601 cggtgccgtc accgtcgcag tccgcatgtt tgaagttgac cgcgctgaca aaccatccgt
      661 tgaaatcggt agcggcctgt gcggtccagc tcagtgtagc gccggcacgt accggaccgg
      721 tctggccgaa ggccaccccg atatcaagga tgtcgctgtt attgcatact aagtcgtagt
      781 tggcatcacc cggccataca ttgctcacga cggtacaggg cgcgtatgtc catccggtgt
      841 taccaccgag atcgacacaa ccattgcccg cgttgaaggt cgcgccgccg cctaccgtca
      901 cgtcctgcag aagcgcattc gcaatacaca cctgcccgga agttttgttc agtagcgcga
      961 tcggattgcc tttcaggaag accgggaaag cgggtgttcc attgcagacc aactcggaga
     1021 agttaatgtt acctcccaga ttcgaggtct tgccggggtt gttcaggaag atggtgtcgc
     1081 agacgatatt tccgctcatg gcgaagtcgt cgaagaaaat ggccttccgg atggaatcac
     1141 tggccaactg cgaatacacg ttcacgtact gataggcggt gaactgttcg cagacgaaac
     1201 tattgaggaa gaaccgcgcg ccgatatcga gtaccgtgat gttgcggaaa gcgccggtgg
     1261 ttcggaaatc ataacccaaa ctgctgtata cgggtgccag ccaacgcatc accacgtcgg
     1321 cctgacggga attgctgaga cttccctgca ggtagaaata tgataccgtc agcgtcatcg
     1381 gcccgaagtt gacatcggtg cccaaattgc ttgtcatccg gctcatggtg atatcattcc
     1441 cgtcggcatc caggttgcca ttcaggagct gcagttggat tccggcacat gcatccagca
     1501 gcgtaaccgt gccggttcca ttgatcgtta ccgaatccaa ctccgtggcg ttgcacgtca
     1561 gatcgatgcc ggaagctccc ggcagcaggt tcgtggtggc tacgttccag ttcatcaccg
     1621 gtgccagtgc aaggtcgccg gtgacgctga tggtcgtacc gttgacggtg atatcactcc
     1681 cggccgcgga gaagtcgatg cccgcagcgc tgacgttggt gacgtcgagt gtcagtattc
     1741 cgctggcgag accggaattg ccatcgaaga tcacctgatc gattgccgct ggtacacagg
     1801 cgccggatgc gccgccggtc gagagcgacc aatgcgttgg atcactccag ttgccagaac
     1861 cgccaaccca gtacaccgtg cgcggagccg gtgagttgat ggtccagccg gtattattgc
     1921 caccgtccac ggaattgttt gcggtaaagc tcgcgcctcc gcctgccgct acactgttga
     1981 gtgaaacgta gtcgacggta atgctgccac ttgtcatgct gatgtttgtc ggtgttcccg
     2041 acaccgtgga acggatgcct gcgaggccgt tacaatcggc ttgaacctgc aggttggtcc
     2101 ccagggtgag ggtttctccg ggcgcttgtg aataattgaa gccgccgttg ctgagtcgca
     2161 aatcgtcgat cgagttgctg ccgctaaccg ttacgtcatg atagaactgg gcgaatccga
     2221 tcgtattggc attgccgata tcgagtgcct gatccgcgac aagcgaggcg actgtgacgc
     2281 tgtcggacag gaatccccgc tcaatgaagt acatattgcg aaactggctg gcccggttgt
     2341 ttcggcaata cgcgggtcct ttatcaaaga tgatgtcggt ggttccggag ccgatgaagg
     2401 ttccgtcgaa gtacatgtcc ttcaggtaga acgtgctgtt ggccgtaatg gcttgtgcgg
     2461 caggataacc cgacagcaac cgggtgttga cattctgacc gttgtaatcg acgttcccgt
     2521 tttgctgata gatccattcg gcatacagcg agtcctgcaa cgtataccaa caggagtcgc
     2581 cataggtcag gtacataaag ggcgtgccgg ttgtgcgcag gatgctgccg gttctgggag
     2641 agcgcaggtg agtttggtaa aggcgccagt tcatctggtg gttgaggtcc agagaaccgt
     2701 acagggtgat gtagtcgccg aagaaagtcg gcgacccggt aacgccgttc acggtaaggt
     2761 cgccggcgta ctgttccagg tgaggaagat aaacggtgtc gccggcgcca agtgaattcg
     2821 cgtcgaagaa gacattgtcc aaacgattgg gggtacaacc
//
LOCUS       DAOTON010000321         2859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27324_length_2859_cov_3.282454, whole genome shotgun sequence.
ACCESSION   DAOTON010000321 DAOTON010000000
VERSION     DAOTON010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27324_length_2859_cov_3.282454"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="P5292_07110"
     CDS             <1..169
                     /locus_tag="P5292_07110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRS38934.1"
                     /translation="EVYTTLGGTPFLDKNYTVFGEVIEGLDVIDKIAAVQTQPGDRPV
                     QDVRMKMTVVQ"
     gene            338..1369
                     /gene="pheS"
                     /locus_tag="P5292_07115"
     CDS             338..1369
                     /gene="pheS"
                     /locus_tag="P5292_07115"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRS38935.1"
                     /translation="MSSLSTYLHEITSFSATTKEEVEQFRLRFLSKKGILAELFAAMK
                     SIPAEEKKQYGAQVNELKQAAEAKLASFQEMLEAAADQAAATDIDLTLPAEPLTLGAR
                     HPISLIRERILQIFSRIGFTVADGPEIEDDWHNFTALNFPENHPARDMQDTFFIEQRG
                     GSDSIALRTHTSSVQVRVMESTKPPIRMLMPGRVYRNEAISARAHCFFHQVEGLYIDE
                     GVSFADMKQTLLYFSREMFGADTEIRLRPSYFPFTEPSAEMDITCELCKGKGCNVCKY
                     TGWLEIMGCGMVDPNVLQNCGIDPERYSGFAFGMGIERIAMLKYDVKDLRLFSENDLR
                     FLRQFSAEN"
     gene            complement(2550..>2859)
                     /locus_tag="P5292_07120"
     CDS             complement(2550..>2859)
                     /locus_tag="P5292_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS38936.1"
                     /translation="IVNVPPRLKRFDISAYITAPITAIDDGGLRQLRLLATPNGYVLE
                     HAGIQGEFSLVNLSGRKCREERLGQGSTRIERDRLSSGAYLLTVRSANGSSRTFRIVF
                     "
BASE COUNT          651 a          873 c          714 g          621 t
ORIGIN      
        1 ggaagtgtac accacgctcg gcggtacgcc ctttctcgat aagaactaca ccgtcttcgg
       61 cgaagtgatc gagggcctcg acgtcatcga caagatcgcc gccgtccaaa cccaacccgg
      121 cgaccgtccg gtgcaggatg tgaggatgaa gatgacggta gtgcaatagt agtttttggt
      181 ttcgcgtttc gggtttcgcg tttccggtta aagttgacca ggactggaaa ctggaaacgc
      241 gaaactggaa accaagaact catttccctc gtccctgcct acgctaaagc ttcggcagac
      301 aagcctcgtc cctcgtccct cgtccctcgt ccctcgcatg tcctccctct ccacctacct
      361 ccacgaaatc accagcttct ccgccaccac caaagaagag gtggagcagt ttcgtttgcg
      421 cttcctgagt aagaagggca tcctggcgga gctgttcgcg gcgatgaagt cgattcccgc
      481 cgaggagaag aagcagtacg gcgcgcaggt caacgaactg aaacaagccg ccgaagcgaa
      541 gctcgcttcc ttccaggaga tgctcgaagc cgcagccgat caggccgccg cgaccgacat
      601 cgacctcaca cttcccgcgg agccgctgac cctcggcgcg cgccacccca tctcgctcat
      661 ccgcgaacgc atcctgcaga tcttttcgcg catcggcttc accgtcgccg acggacccga
      721 gatcgaagac gactggcaca atttcaccgc gctcaacttt ccggagaatc accctgcccg
      781 cgatatgcag gatacgttct tcatcgagca acgcggcggc agcgattcca tcgcgctgcg
      841 cacgcacacc tcgagcgtgc aggtgcgcgt catggaaagc accaagccgc ccatccgcat
      901 gctgatgccg ggacgcgtct accgcaacga agccatcagc gcgcgcgcgc actgcttctt
      961 ccaccaggtc gaaggacttt acatcgacga aggcgtttcc ttcgccgaca tgaaacagac
     1021 gctgctgtac ttctcccgcg agatgttcgg cgccgatacg gaaatccggt tgcgtccgtc
     1081 ttactttccg ttcacggagc cttccgccga gatggacatc acctgcgaac tttgcaaagg
     1141 caaaggatgc aacgtgtgca aatacaccgg ctggctcgag atcatgggtt gcggcatggt
     1201 cgatcccaac gtactgcaga actgcggcat cgatcccgaa cgctacagcg gtttcgcctt
     1261 cggcatgggc atcgaacgca tcgccatgct gaaatacgac gtcaaagacc tgcgcctctt
     1321 ctcggagaac gacctgcggt tcctgcgcca gttctcagca gaaaattaac cagagaccgc
     1381 tttgtctgtg tccctctgcg cattcctctg cgtccctctg tgttactttt atcttagtgt
     1441 aattcaaaca cttagattat gataaagaaa aaacagaaac caaatatccc accacttgtt
     1501 ctctatacaa cctccttaaa caggtggtca taaagtgtgg aaatggtgaa aagccgggcg
     1561 tgagtccggc ttttttttgt tgtagatcaa cgggcccgaa tgaatgcagg gacgaggggc
     1621 gagaccgagg gacgagggag acggttgatt gttccgagtt ccctgcctac gctaaagctg
     1681 cggcaggcag gcgcgttccg agttagtatt tattctcagc ctacctgtaa gcaacagcaa
     1741 gcacgattca ctgcttactg cctactgcct actgcctact gcctactgcc tactgtaatt
     1801 aagagcggac caaccgtacg ctcctcccct tcagtcccgt gttcactcgt cgcaacaatg
     1861 ccaatgcaat gatggacgca ggtagcgcgc ctcgctcacc cggggctagc ctggaacgtt
     1921 ccggcttcga ctcaacgggc accgtcttgc tgtaattcca ggctgcttgc ccgatgaacc
     1981 cattatgcga cccctacggg gccgaaataa aaaaatgaat tcgtgatgct aggattatta
     2041 gacccctacg gggtcatcgt tatatgatcg actggcgata aaatgttctg atgtcaatcc
     2101 tgattattgc atgccaaaaa tccataatat cagaaaaccc gcggggtcat tgttagctca
     2161 acgactggcg ttgcgttaat ccgcccgccc aggcatgcct gggcagacta caggcatcga
     2221 caaactgttc actaaaaaac tattcgctat tcgctattgg ctattcgctc cacactattc
     2281 actatttact gaaacaaaat ctcccgactt cgacacaacg ggaccgatct gcggccattt
     2341 catgccgcgt acctgatgaa tcgtttccgc gacccctacg gggccgaaat aaaaaatgga
     2401 atctcggtgc taggataccg tgacccctac ggggtcatcg ttatctgatc gactggcgtt
     2461 gcgttaatcc gcccgcccag cctaggcagg ctacaggcaa aggcaaactg cctactgcct
     2521 actgcctact gcctactgtt cactgctcac tagaaaacaa tccgaaatgt cctgctggat
     2581 ccgttcgctg atcgaaccgt caacagataa gcgccgctgc tgagccggtc gcgctcgatg
     2641 cgcgtgcttc cctgtcccag tcgttcctct ctgcattttc ttccggacag attcacgagc
     2701 gagaactcgc cctgtatacc ggcatgttcc agcacataac cgtttggtgt ggcgagcagg
     2761 cggagctgac gaagtccgcc atcgtcaatc gcagtgatcg gagccgtgat gtacgcgctg
     2821 atgtcgaacc gcttcaaccg cggcggcacg ttcacgatc
//
LOCUS       DAOTON010000322         2857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27348_length_2857_cov_2.803712, whole genome shotgun sequence.
ACCESSION   DAOTON010000322 DAOTON010000000
VERSION     DAOTON010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27348_length_2857_cov_2.803712"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1266
                     /gene="cysN"
                     /locus_tag="P5292_07125"
     CDS             7..1266
                     /gene="cysN"
                     /locus_tag="P5292_07125"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HRS38937.1"
                     /translation="MVNRGESELLRFTTAGSVDDGKSTLIGRLLLDTRNIFDDQLAAI
                     ESTSKQRGFDYVDLSLFTDGLKAEREQGITIDVAYRYFATAKRKFIIADTPGHRQYTR
                     NMVTGASTANLALILIDARKGILEQTCRHAFIASLLRIPHMIVCVNKMDLVDYNQETY
                     DRIVEAFRDFSSRLDIQDVQFVPISALKGDNVVERSNRMPWYEGSTLLYHLENVHISG
                     DINHVDCRFPVQYVIRPQQDDFHDYRGYAGRLAAGVFREGDRVMVLPSGFTSRIASID
                     TYEGQVQEAFAPMSVSIRLEDDIDISRGDMIVRENNVPQSGQDLDVMLCWLHERPMQA
                     GGRYVLMHTTREARCIVKDIRYKIDITTLRRLEQERTFGMNDIGRVQLRTTVPLSYDS
                     YRRNRLTGSLILIDEHTNETVGAAMIL"
     gene            1292..2095
                     /gene="cysQ"
                     /locus_tag="P5292_07130"
     CDS             1292..2095
                     /gene="cysQ"
                     /locus_tag="P5292_07130"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRS38938.1"
                     /translation="MTAISDLLITAITASIEAGRAIMRLYSGDIQVELKDDRSPVTAA
                     DKAAHAVIQQALVETGLPILSEEGKEIPYATRKSWSRYWLVDPLDGTKEYIARTGEFT
                     VNIALMENDRPVAGVIYAPVIDVLYAGDVAGHAFHLIQDASRINRNSLHRLGDFRVAS
                     QPASVTGGIRVVASRSHRTPETDRFIEELKQEFGSAALVSAGSSLKFCLVAEGKADIY
                     PRFGRTMEWDVAAGHAILLAAGKDVRSYPEYAIFPYNKPDLGNGWFIAR"
     gene            2190..>2857
                     /locus_tag="P5292_07135"
     CDS             2190..>2857
                     /locus_tag="P5292_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRS38939.1"
                     /translation="MSTGFLVTWITARFYGADALGVVSICVAILSLASVFGKLGMDVA
                     LMRFITGFATRQQFDSIKGVYVTAMRHVVPISLGIAVLLWLLAEPMADQLFHKPDLAP
                     VLRINAWCTLPLVLLLLHSEAVRGLKHIGSYTFFQTAAVSTAATLLLFLMMTVATGRF
                     VPVYIQFVCITLVGLASCLIWLRGSRFFQSATRHEVSGQQLRATAGPMFTTTIMQLLM
                     SWAGT"
BASE COUNT          601 a          757 c          815 g          684 t
ORIGIN      
        1 tcagctatgg ttaatcgagg cgaatcggag ttgttgcggt ttacgactgc aggcagtgta
       61 gatgacggaa agagtacgct cattggccga ttgttactgg acaccagaaa catcttcgac
      121 gatcagctgg ccgcgatcga atcaaccagc aaacagcgcg gattcgatta tgtggacctg
      181 tcgctattta ccgacgggct aaaagcggag cgcgagcagg ggatcaccat cgacgtggcg
      241 tatcgttatt tcgccaccgc taagcggaag ttcatcatcg cggatacgcc ggggcaccgg
      301 cagtataccc ggaacatggt caccggtgcg tccaccgcca atctcgccct catcctgatc
      361 gacgcgcgga aaggaatatt ggaacagacc tgccgccacg cattcatagc ctcgctgttg
      421 cgcattccgc acatgatcgt ttgtgtcaat aagatggacc tggtcgacta caaccaggag
      481 acctatgacc gcatcgtgga ggcttttcgc gatttttctt cgcggctcga tattcaggac
      541 gtgcaatttg ttcccatcag tgctttgaag ggcgataatg tcgtggaacg atcgaacagg
      601 atgccctggt acgaaggttc cacgctcctg tatcatttgg aaaacgtcca catcagcgga
      661 gacatcaacc atgtcgattg ccggttcccg gttcaatatg tcatccgacc tcagcaggat
      721 gattttcatg actaccgggg ttatgccggt cggttggcgg ctggtgtgtt ccgggaagga
      781 gaccgtgtaa tggtcttacc gtccggattc acttccagaa ttgcatcaat cgatacctac
      841 gaagggcagg tgcaggaagc ctttgccccg atgtcggtca gtatccggct ggaagatgat
      901 atcgacatca gccggggtga tatgatcgtt cgggagaata acgttccgca gagcggccag
      961 gatctcgatg taatgctctg ttggctgcac gaacgaccga tgcaggccgg cggacggtat
     1021 gtgttgatgc acactacgcg ggaagcacgg tgtatcgtaa aagacatccg gtacaagatc
     1081 gacatcacga ccttgcggcg gctggaacag gagcggacat ttggcatgaa tgatatcggt
     1141 cgggtacaac tgcggaccac ggtgccgctt tcgtacgaca gttatcgtcg gaaccggttg
     1201 acaggtagcc tgatcctgat cgacgaacac acgaacgaaa cggtcggagc ggccatgatc
     1261 ctttgaaaca aacgtttagc acatccagtt cgtgacggca atttccgacc tactgatcac
     1321 cgcgatcact gcttccattg aagccgggcg cgctatcatg cgcttatatt ccggcgacat
     1381 ccaggtagag ttgaaagatg atcgcagtcc cgtcaccgcc gcggataagg ctgctcacgc
     1441 ggtcattcaa caggctttgg ttgaaaccgg tttaccaatc ctgagcgagg aagggaagga
     1501 gatcccatat gctacccgga aatcgtggag ccgctattgg ctcgttgatc cattggacgg
     1561 aaccaaagag tatatcgcgc gcaccggtga gttcacggtc aatatcgcgt tgatggaaaa
     1621 tgaccggccc gtcgccggcg tcatctatgc tcctgtgatc gatgtgttgt atgcgggcga
     1681 tgtggcgggg catgcgttcc atcttattca ggatgcatcc cggatcaaca ggaattcgct
     1741 ccatcgactc ggcgatttca gagtggcttc ccagcctgca tctgtcacag gcggtattcg
     1801 ggtggttgcc agtcgctctc atcgtacgcc ggaaaccgac cggttcattg aagagctaaa
     1861 acaggagttc ggttcagcag cgctggtttc cgccggtagt tcactaaagt tttgcctggt
     1921 agcggaaggc aaggcggata tctacccgcg gtttgggcgt acgatggaat gggatgtcgc
     1981 cgcgggccac gcgatcctgc tggctgcggg caaagatgtc cggtcttatc cggagtatgc
     2041 cattttccca tacaataaac ctgatctcgg taatggctgg ttcatcgcgc gataggatat
     2101 cggatctatg gcagcgatac cgtcaggatc ctgctcaccg tgaactgctc cattccagtt
     2161 tcatttctct actgatccga ggattgggcg tgtcaacggg cttcctggtc acctggatta
     2221 ccgctcgatt ctacggtgcc gatgcgttag gcgtggtttc gatctgtgtt gcgattctca
     2281 gtctcgcgtc ggttttcgga aaactcggca tggatgtcgc gttgatgcgc ttcattaccg
     2341 gttttgcaac ccggcagcaa ttcgattcca tcaaaggggt ctatgtgacg gcgatgcggc
     2401 atgtcgttcc gatttcgttg gggatcgccg tgctgctctg gttacttgca gagccgatgg
     2461 ccgaccaact gtttcacaag ccggatcttg ctcctgtcct tcggatcaac gcatggtgca
     2521 cccttccgct cgttctcttg ctgttgcatt cggaggcggt gcgtgggttg aagcacatcg
     2581 gatcctacac ctttttccaa accgccgcgg tatccactgc tgcgacgctg ctgttgtttc
     2641 tgatgatgac ggtcgctacc ggccgattcg tccctgtgta tatacagttc gtatgcatca
     2701 cgctggtcgg cctggcatcc tgcctgatct ggctcagggg aagcaggttc tttcaaagtg
     2761 ccacccggca cgaggtgagc ggtcagcaat tgcgcgccac cgccggtcca atgttcacca
     2821 ccacgatcat gcaattgctg atgtcctggg cgggcac
//
LOCUS       DAOTON010000323         2844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27517_length_2844_cov_3.116888, whole genome shotgun sequence.
ACCESSION   DAOTON010000323 DAOTON010000000
VERSION     DAOTON010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27517_length_2844_cov_3.116888"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="P5292_07140"
     CDS             <1..806
                     /locus_tag="P5292_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS38940.1"
                     /translation="TTEQGRSSRRLSDLYNELMKSYDKLLANNEKLLSGNTEETRKLI
                     SQLNDLQKDLQLKEDRLRRDSLALLEREGKLGELRTSLEDRQRRVDELQGILGKSDST
                     VGALKKAVSDALLGFENKGLTVKQDGSRVYVSMEERLLFASGSTAIEKEGESALQELA
                     KVLEKNPDINVTVEGHTDNIPINGVLPSGARDNWELSVLRATSVTKILLKGTTIDPLR
                     VTAAGRGPYMPVDPTNTTEARKKNRRTEVILSPKFDELLKVLEQLGQQQ"
     gene            complement(905..1906)
                     /locus_tag="P5292_07145"
     CDS             complement(905..1906)
                     /locus_tag="P5292_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38941.1"
                     /translation="MKKALLLLLLVPLSLTEVAAQKAAVVAVSTGVTNYMGDLGNEKY
                     FPFSSLSNGANVTLRNFLGGGNSVNSTFDLQVRLSWHRLQYDETKPLNGKQGMDLRNY
                     LRGLSFRNDLFGTEVGITYNIYPNKATPLWKPRWSFFVMAGIGAYYGTPRADLFNGSQ
                     DLSNRYYFWNDGTIRNAPQNAQGIGQEIKKDGKYETNLRDFYTEGQGYNNEIHRKAPY
                     ELCNLGVPTGFGVRYHYNKMLTLSAEFNYYYLITDYADDVSGRYATYEELRAAFPDDA
                     TFELAKYISDPTGRGTNGFIGPVTSPRGNPSKNDSFTYVSLEAAWKFYYKKKGIYGQ"
     gene            2124..>2844
                     /locus_tag="P5292_07150"
     CDS             2124..>2844
                     /locus_tag="P5292_07150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRS38942.1"
                     /translation="MKSHYQVLIIGGGTGGIMTAAQFRRKRPTASIAIVEPNEDHWYQ
                     PAWTLVGAGTFDFEATRRKESTVIPAGVDWIKDKATVFLPEENKIRTEKSGEISYDYL
                     IVSAGIKMDIDALPGLREALQTDFVCSNYLDPRKTYRLLKNFKGGNAIFTQPTTPIRC
                     GGAPQKIMYMAEEYLRKHGLREKTNVLFATPGSVIFGVKEFADTLNSIIRSRKIIFKP
                     FYAPVRIDPVKREIIFKYVNPN"
BASE COUNT          692 a          764 c          773 g          615 t
ORIGIN      
        1 acacgaccga gcagggtcgt tcctcgcgtc gactgtcgga cctgtacaac gagctgatga
       61 agtcatacga caaactgttg gccaacaacg aaaagctgct ttcgggtaat acggaagaaa
      121 cccgcaaact gatctcacag ctcaacgatc tgcagaagga ccttcaactg aaggaggatc
      181 gtttgcgccg cgactccctt gctctcctcg agcgggaagg caaactcggc gaattgcgca
      241 ccagcctgga agaccggcag cgccgcgtcg acgagcttca gggcatcctg ggcaagagcg
      301 actctacggt cggcgcactg aagaaagcag tcagcgatgc cttgctgggc ttcgagaaca
      361 aaggcctgac cgtgaaacaa gacggcagtc gggtgtatgt ctccatggaa gagcgcctgc
      421 ttttcgcttc cggaagtacg gccatcgaga aagaaggcga gagcgcttta caggagttgg
      481 ccaaagtgct tgagaagaac cccgacatca acgtaaccgt cgaagggcat accgataaca
      541 tcccgatcaa cggtgtcctg cccagtggcg cgcgcgacaa ttgggaactc tccgtactgc
      601 gcgcgacttc tgtcacgaag atcctcctga aaggaacgac catcgatcca ctgcgtgtca
      661 ccgcagccgg tcgtggcccg tacatgccgg tagatccgac caatacgacc gaagccagga
      721 aaaagaaccg cagaacggaa gtgatcctga gtccgaagtt cgacgaattg ctgaaagtgc
      781 tcgagcaact cggtcaacag caataaagcg gatgtggctt caacgaaaag cgaaaggctg
      841 cccttgttcc tcagggcagc cttttccgct atgaacggag cagttcaagg tgtttcatgt
      901 caagttactg tccgtaaata cctttcttct tgtaatagaa cttccaggca gcttccagcg
      961 agacgtaggt aaatgaatcg ttcttcgatg gattaccgcg cggactggtg accggaccga
     1021 tgaaaccgtt cgtgccacgg ccggtcgggt cggaaatgta tttcgcgagc tcgaaggtag
     1081 cgtcgtccgg gaaggccgcg cgaagttcct cgtaggtggc gtagcgacca ctcacatcgt
     1141 cggcgtaatc ggtgatcaga taatagtagt tgaattccgc cgagagggtc agcatcttgt
     1201 tgtagtgata ccggacgccg aagcccgtcg gcacaccgag gttgcacagt tcgtacggcg
     1261 ccttgcggtg gatctcgtta ttatagccct gtccctcggt atagaaatca cgcagattgg
     1321 tttcgtactt cccgtctttc ttgatctcct gcccgatgcc ctgtgcattc tgcggagcat
     1381 tccggatggt gccatcgttc cagaagtagt agcgattgct cagatcctga ctgccgttga
     1441 acaggtctgc tcgcggtgtt ccgtagtagg cgccgatacc ggccatcacg aaaaagctcc
     1501 aacgcggctt ccacagcggg gtggccttgt tggggtagat gttgtaggta atgccgactt
     1561 cggttccgaa gaggtcgttt cggaaactca ggccgcggag gtaattgcgc aggtccatgc
     1621 cctgcttacc gttcagcggt tttgtctcgt cgtactgcaa tcggtgccaa ctcaggcgca
     1681 cctgcaggtc gaaggtactg ttgaccgaat ttccgccgcc gaggaaattt cgcagcgtga
     1741 cattcgcgcc attgctcagg gagctaaagg gaaaatactt ctcgttaccc aggtcaccca
     1801 tgtaattggt aacgccggta gagactgcca cgaccgcggc tttctgagcg gccacctccg
     1861 tgagcgagag gggcaccaac aggaggagaa ggagagcttt cttcatgaca cacacgcctg
     1921 ttttagaaca agtcgtggaa gataacggtt ttcctgaaag ctgcaacccc gattttggcg
     1981 aactaatcaa aaggtcgggt gatcgcattt tcccgttatt tgaacgaatt ttcatatgtg
     2041 acgaatttca ggttccaacg tgaggatggt cacatagtaa aacccatatc tgaactttct
     2101 ttgatgtacc aattcagcat atcatgaaaa gccattatca ggtactcatc atcggcggcg
     2161 gaacaggcgg catcatgacc gcagcccaat tccgccggaa acgtcctacg gcttccatcg
     2221 ccatcgtgga accgaacgag gatcactggt atcagcctgc ctggacgctg gtgggtgccg
     2281 gaacgttcga tttcgaagct acccgcagaa aagaatcgac cgtgattcca gctggggtgg
     2341 attggatcaa ggataaggcg acggtttttc ttcccgaaga gaacaagatc cgtacggaga
     2401 agtcggggga aatctcttac gattacctga tcgtaagcgc cggaattaaa atggacatcg
     2461 acgccctgcc gggtttgcgc gaagctttgc aaaccgattt cgtttgcagc aactacctcg
     2521 accctcggaa gacctaccgg ttactcaaga atttcaaagg cggcaacgcg attttcaccc
     2581 agcctaccac ccccatccgc tgtggcggcg cgccacagaa gatcatgtac atggccgaag
     2641 agtacttacg caagcatgga ttacgagaga aaacgaatgt cctcttcgcc acgccgggtt
     2701 cggtcatttt cggtgtgaag gaattcgccg acacgctcaa cagtatcatc cgcagtcgga
     2761 aaatcatctt caaacccttt tatgctcccg tacggatcga tcctgtcaaa cgcgagatca
     2821 tcttcaaata cgtcaatccg aacg
//
LOCUS       DAOTON010000324         2844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27519_length_2844_cov_2.986734, whole genome shotgun sequence.
ACCESSION   DAOTON010000324 DAOTON010000000
VERSION     DAOTON010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27519_length_2844_cov_2.986734"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2024)
                     /locus_tag="P5292_07155"
     CDS             complement(<1..2024)
                     /locus_tag="P5292_07155"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRS38943.1"
                     /translation="MRRFFSRIANRHEQVFKYFLFLLTVLLIVVALPKETQFNYTYQK
                     GKPWPYDNLLAPFDYAMTKNEAELNEERAEVLRTIHPFYRFNTATEAERIDLFRKSLS
                     ETWSAKYANGKRSPREQEQLERNRGLQESIGTEVLRMVFRKGVILLSDQPAGRNDDGM
                     LTLVNGNVADERPVTDFFTIQTAYEAINDQLNSLTAADQALLAPLLENSLAHNVLFDD
                     QATRQWTRQVLDKISTTRGLVQKDEVVILKGEVVDDNRFQRLESLKAEMKNQEITGAS
                     RWMMFLGHFLLVSLAISMLILFLYQFRKELYDDLAKITLLLLLLLLTTYVFLWARKVN
                     LFDMYMVPVCILPIVIRAFYDTRTALFTHIVSLLIIGFEAPSGFEFMFIQTIAGMVAI
                     FTIVSMSRRVQFFISVLMIFAAYSIAFVGVTILHEANFRSIDWMNLKWFAANSVLTLF
                     AFPLIFIFEKTFGFLSDVSLMELADSNSPLLRELAYKAPGTFQHSLQVANLAEAVTFK
                     IGGNPLLVRAGALYHDIGKMERPLYFIENQNSEFNPHDELSFEESARIIKDHVIRGIE
                     IAKQRKLPEPIIDFIRTHHGTSVLQYFYHSFLKNFPEKVPNEELFHYPGPLPFSRETV
                     VLMMADSVEAASRSLKNATAENISNLVDNIIDHQMSQGQFINAPITLKDI"
     assembly_gap    2027..2036
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2165..>2844
                     /locus_tag="P5292_07160"
     CDS             2165..>2844
                     /locus_tag="P5292_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRS38944.1"
                     /translation="MKEVYIVSAVRTPIGSFGGTLSALSAPQLGSHAIKAALERIKLD
                     PKEVQEVYFGNVLSAGVGQAPVTQASIGAGLPNTVPGTTINKVCASGMKAVALAAESI
                     LLGDNDCVVAGGMESMSNVPYYLDKARNGYKLGHGSVTDGLVKDGLWDVYKDYHMGNA
                     AELCATECKISRAEQDAYALESYRRSVEAYKNGAFKDEIAPISVPVRGKDPIVVSEDE
                     EYKNLKAD"
BASE COUNT          701 a          723 c          782 g          628 t
ORIGIN      
        1 ctgatgtctt tgagggtaat gggagcattg atgaattgac cctggctcat ctgatgatcg
       61 ataatattgt cgaccaggtt ggagatattc tccgccgtgg cgttcttcaa actgcgggat
      121 gcagcttcca cggaatcggc catcatgagc acgaccgtct cccgggaaaa gggcaacgga
      181 cccgggtaat gaaacaattc ctcgttgggt actttctcag ggaagttctt cagaaaactg
      241 tgatagaaat actgcagtac gcttgttcca tgatgggtcc ggataaaatc gatgatcggc
      301 tccggaagtt tccgttgttt ggcgatctcg attccgcgaa tgacgtggtc tttgatgata
      361 cgggcgcttt cttcaaacga gagttcatcg tgcggattga attcggagtt ctggttttcg
      421 atgaaataaa gcgggcgctc catcttgccg atatcgtgat acaaagcacc tgcgcggacg
      481 agcagtggat taccaccgat cttgaatgtc accgcctcgg ccaggttggc tacctggagc
      541 gaatgctgga aggtcccggg ggccttatag gccaattccc gcagcaaggg ggaattggaa
      601 tccgccagtt ccattaagga gacatcggac aggaaaccaa aggtcttctc gaaaatgaaa
      661 atgagcggga aagcgaacag tgtcagcacc gaattggctg cgaaccactt gagattcatc
      721 cagtcgatgc ttcggaagtt cgcttcgtgc aggatggtca cgccgacgaa ggcgatcgaa
      781 taagccgcga agatcatcag aacgctgatg aaaaactgca cccggcgact catgctgacg
      841 atcgtgaaga tcgcgaccat gccggcaatg gtttggatga acatgaattc gaaaccactc
      901 ggggcttcga acccgatgat cagcaggctg acgatatggg tgaacagagc cgtccgcgta
      961 tcataaaaag cacgaatgac gatcggcaga atgcagaccg gcaccatgta catgtcgaag
     1021 aggttgacct tcctggccca caggaataca taggtcgtga gcagcaacag caacagcaaa
     1081 agggtgattt tggccaggtc gtcatacaat tccttccgga actggtacag gaacaatatc
     1141 agcatcgaaa tcgccagcga aacgagtagg aagtgtccga ggaacatcat ccagcgggaa
     1201 gcgccggtga tctcctggtt cttcatctcc gccttgagcg attccagtcg ctggaaacgg
     1261 ttgtcgtcga ccacctcccc tttcaggatc acgacttcat ccttttggac caggccacgg
     1321 gtggtggaga tcttatcgag cacctgtcgg gtccattgac gcgtggcctg atcgtcaaag
     1381 agcacgttgt gcgcgagtga attctccaac aaaggcgcca gtagcgcctg atcagcggca
     1441 gtcagtgaat tgagttgatc attgatcgct tcgtacgcgg tctggatcgt aaagaagtcg
     1501 gtgaccggcc gttcatccgc cacgttgcca ttgaccaggg tgagcatgcc gtcatcattt
     1561 cttcctgccg gctggtcgga caacaagata acccccttcc tgaaaaccat ccgcagcact
     1621 tcggttccga tgctttcctg taagccgcga ttacgttcca gttgttcctg ttcccggggg
     1681 gatcgctttc cgttcgcata cttcgccgac caggtttcac tcaggctttt cctgaataag
     1741 tcgatccttt ccgcttccgt cgcggtattg aaccggtaaa agggatgaat ggtgcgcagg
     1801 acttccgccc gctcttcatt gagttccgcc tcgttcttcg tcatggcata atcaaaggga
     1861 gccagcagat tatcgtatgg ccagggttta cccttctgat aggtatagtt gaattgcgtt
     1921 tccttgggaa gcgcgacgac aatcagcaat actgtcagca ggaacaggaa atacttgaaa
     1981 acctgttcat gtcggttggc gatccgggaa aaaaaacgac gcatggnnnn nnnnnntgtt
     2041 ctcgacagga cgaatctgca taaagtaacc aggctcctgc gaatggtcgg agggaaattt
     2101 caacaggctc cggctaacac cgtgggacta agtttgctac ctttgcacac ccaaaaccaa
     2161 gaccatgaaa gaagtctata tcgtctccgc agttcgtaca ccgatcggaa gtttcggcgg
     2221 taccttgtcc gccctatccg ccccacagct tggctcccat gccatcaaag cggcgctgga
     2281 gcgcatcaag ttggatccga aagaagttca ggaagtttat ttcggcaatg tactctctgc
     2341 cggagtcggt caggcaccgg tcacccaggc ctccatcggc gcagggcttc cgaacaccgt
     2401 acccggtacg acgatcaata aagtatgtgc ctccggcatg aaagcggtag cccttgctgc
     2461 agaatcgatc ctgctgggcg acaatgattg cgtggtcgcg ggaggaatgg agagcatgag
     2521 caatgttccc tactacctcg acaaggcacg gaacgggtac aaacttggac acgggtcggt
     2581 tacagacgga ctggtgaagg acggactgtg ggatgtgtac aaggattatc atatggggaa
     2641 cgctgccgag ttgtgcgcca ccgagtgcaa gatcagccgc gcggaacagg atgcgtatgc
     2701 cctggaatcc taccgacgtt cggtagaagc ctacaagaac ggcgccttca aagatgagat
     2761 cgccccgatc agcgtgccgg tacgcggaaa agatccgatc gtcgtatcag aagatgagga
     2821 atacaagaat ctcaaggccg ataa
//
LOCUS       DAOTON010000325         2843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27534_length_2843_cov_3.304878, whole genome shotgun sequence.
ACCESSION   DAOTON010000325 DAOTON010000000
VERSION     DAOTON010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27534_length_2843_cov_3.304878"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..>2843
                     /locus_tag="P5292_07165"
     CDS             6..>2843
                     /locus_tag="P5292_07165"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRS38945.1"
                     /translation="MLLLCFSLQRSEAAVVFNEFMSNNQGTVTDSDGDYSDWIELYNT
                     SATPVNLLNWGLSDNVDSLFKWRFPNYTLNAGSFLRIWCSGKNRAVTTPFHTSFSIDS
                     RGETIYLSDSTGAVQDNLLAIYMPPDRSFGRLPNGSVNQFYLSVATPAASNNFATAFQ
                     GAVTEPVTFSLAGGFYPSAQTFTLSHPDPTVTIRYTLDGSEPTANSTPYTGPITVDSR
                     AADTNFYSTIRTCYKVHAWLPDWNPPLGNVYKCNVVRARAFKSSFLPGPVSTQSYFID
                     PAMATRYGSLPVVSLVSDPRNLFNDTVGIYVPGINYVPNTFQANYYFDWNRQANVEFF
                     MPGGQQVVNSNFRISINGVTSRSSPQKGLDVTATTDLGEGKIRYPFFANTPGPARYLT
                     SFDKLKLRSWGSDRAFALFRDAYTAQFFHKTTLDYEAYRPCVVFIDGEYWGLHELRER
                     NRNEEYFEDHYLIDKDNPGFDIVDLQDNIAIEGDTLAWSQLEQFVTTNNMADSANYAY
                     VKTKMDVENFMLNYVSSIYFSRGDWPGQNEAVWRPRTPDGKFKWIQWDMDNTTAYYLN
                     PWYDMFQQAILGSRGYGPSPMLVALLANTGFRDNWINLFADYMNTNFLPALMQAKVDE
                     LRNELSPYMQEYRDRWQCNANWQAQTDSMKWWVNLRQQFCRQQILTTFSLPAFRRVSL
                     NVSDTAKGNIRVNTVFLDPSTPRTTTNVFPWAGYYFQGVPVPLTAVPKPGYRFVRWLQ
                     SNDTNATIQLNLVSDTVLTALFDVDTSYRAPLRVVINEAQASNNFTVQDNYGEYDDWL
                     EIYNPNADTVDLAGYYVTDNLILPTRFQFPVGNDSTKIPPYGHLLLWADDDAEQGVLH
                     LPFKFNASGDLVVLYRPDASSVEDSIRLPSIQSDQSFGRSYDGSATWVFFTTPTPAAT
                     NQEIPAASLLINEVMASNTGTIADNYGQFDDW"
BASE COUNT          621 a          833 c          731 g          658 t
ORIGIN      
        1 tcctgttgct tctcctgtgc ttctcccttc agagatcgga agcagccgtc gttttcaacg
       61 agttcatgtc caacaaccag ggcacggtca ccgattccga tggcgactat tccgactgga
      121 ttgagttgta caatacatcc gccacgccgg tcaatttgtt gaactgggga ttgtccgaca
      181 atgtcgatag tctgttcaaa tggcggtttc cgaattacac gctgaatgcg ggtagcttcc
      241 tgcgcatctg gtgttcggga aagaaccggg ccgtcactac tccttttcat accagcttct
      301 cgatcgattc gcgcggcgag accatttacc tgagtgactc caccggcgct gtacaggata
      361 atctcctggc catttatatg cctccggacc gttcgttcgg tcgtctgcca aatggcagcg
      421 tcaaccagtt ttacctctcg gttgccacac cggctgcatc gaacaatttc gcaacggctt
      481 ttcaaggcgc agtcaccgag ccggtaacgt tttcgctggc cggcggtttt tatccttccg
      541 cccagacgtt caccctgtcg catcccgacc ctaccgtcac catccgttat accctcgacg
      601 gttccgagcc gacagcgaat tcgaccccat acaccggacc gatcacggta gacagccgtg
      661 cggccgatac gaatttctat tccacgatcc ggacctgtta caaagtgcac gcctggctgc
      721 ctgactggaa cccacctttg ggtaatgtct acaaatgcaa cgtggtcagg gcgcgcgcct
      781 tcaagagttc gttcttgccc ggccccgttt ccacgcaatc gtatttcatt gatccggcca
      841 tggcaacgcg gtatggaagt ctgccggtgg tctctttggt ctccgatccg cgcaacctgt
      901 tcaacgatac ggtcggaatc tatgtgccgg gaatcaacta tgtgcccaat acgtttcagg
      961 cgaactacta tttcgactgg aaccgacagg cgaacgtcga gtttttcatg ccgggcgggc
     1021 agcaggtcgt gaacagtaac ttccggatct ccatcaacgg ggtcacttcc cggtcgagtc
     1081 cacaaaaggg acttgatgtc acggcaacga cggatcttgg ggaaggcaag atccgctatc
     1141 ccttcttcgc gaatacaccg ggccccgcgc gttacctgac ttccttcgac aagctcaagc
     1201 tacgcagttg gggcagcgac cgggctttcg cgctcttccg tgacgcttat acggctcagt
     1261 tcttccataa gactacgctc gactatgaag cttatcgtcc ctgtgtcgtc ttcatcgatg
     1321 gagaatattg gggactgcac gaattgcgag agcggaaccg gaacgaagaa tatttcgaag
     1381 accattacct gatcgacaag gacaatcccg ggttcgatat cgtcgatctg caggataaca
     1441 tagccattga gggcgatacg cttgcctggt cgcagctcga acaattcgtc acgacgaata
     1501 acatggcgga ttcggccaac tatgcgtacg taaagacgaa gatggacgtc gagaatttca
     1561 tgctcaacta cgtttcgagc atttacttct cccgtggcga ctggcccggg cagaatgaag
     1621 ccgtctggcg tccgcgtaca ccggatggga agttcaaatg gatccaatgg gacatggaca
     1681 acactaccgc ctattacctg aatccatggt acgatatgtt ccagcaggcg attctcggca
     1741 gcaggggtta cggtccatct cccatgctgg tcgcgctgct ggccaatacc gggttccgtg
     1801 acaattggat caacctgttc gcggattaca tgaacactaa cttcctgccc gcgctcatgc
     1861 aggcgaaggt ggacgaattg cggaacgagt tatcacccta catgcaggag taccgcgacc
     1921 gctggcagtg caacgccaac tggcaggcgc agacggacag tatgaagtgg tgggtcaacc
     1981 tgcggcagca attctgccgg cagcagatcc tgacaacctt ttccctaccc gcgtttcgac
     2041 gcgtatcgtt gaacgtaagc gataccgcca aggggaatat ccgggtcaat accgtttttc
     2101 tcgatccatc aacaccgcgt actaccacca acgtttttcc ctgggcagga tattacttcc
     2161 agggtgtacc ggttccgctc acggcggttc caaaacccgg ctaccgcttc gtacgctggt
     2221 tgcagtcgaa cgacaccaac gcgaccatcc agcttaacct cgtcagcgac acggtgctga
     2281 cggccttgtt cgatgtcgat acttcctatc gtgctccctt gcgggtcgtg atcaacgaag
     2341 cacaagcctc gaacaatttc accgtgcagg acaattacgg ggaatacgac gactggttgg
     2401 agatctacaa cccgaacgcc gacacggtcg atctggccgg gtattatgtc accgataatc
     2461 tgattctgcc gacgcgcttt cagttccccg tcggaaacga ttcaacaaag atccccccgt
     2521 acggacacct gcttttgtgg gccgacgatg acgcggagca aggagttctg caccttccgt
     2581 tcaagttcaa tgcttcgggc gacctggtcg tgttgtatcg tccggatgcc tcgagcgtgg
     2641 aagactccat tcggttaccg tcgatccagt cggatcaatc ctttggccgt tcgtatgacg
     2701 gttccgccac atgggtattt tttacaacgc ccacaccagc cgccaccaac caggagatcc
     2761 cggccgcgag tttattgatc aacgaggtaa tggcttccaa caccgggacg attgccgaca
     2821 attacgggca gttcgacgac tgg
//
LOCUS       DAOTON010000326         2836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27623_length_2836_cov_3.385832, whole genome shotgun sequence.
ACCESSION   DAOTON010000326 DAOTON010000000
VERSION     DAOTON010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27623_length_2836_cov_3.385832"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1284)
                     /locus_tag="P5292_07170"
     CDS             complement(55..1284)
                     /locus_tag="P5292_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRS38946.1"
                     /translation="MDPRRISILDFTYSLPEDRIAQHPLSERDASRLLVYQEGKITDT
                     SFQHLPDFLSPNDLLVFNDTRVIRARLLYRKPTGSRIELLVLDPANGIDPTQALQVTG
                     TAEWLSLIGNAKRWKSEEPLVWELQLAGRAIQLEAIKGDKQDDAYIVRFNWNSPETFG
                     EILEAIGRMPLPPYMDRDEEPEDAERYQTVYARLPGSVAAPTAGLHFTERVFSKLREQ
                     GIGVDWLTLHVGAGTFKPVKSPTMQEHPMHRERLIVRREMIERLLHAHESGRVIAVGT
                     TSLRTLESLYWLGCRVLAGKPEAEVCIGQWEPYESQNDVPVQDALTALLRRMDESNRT
                     AISGHTQLLIAPGYSFRVVDGLVTNFHQPESTLLLLVAAFIGSDWRTVYHHALANGYR
                     FLSYGDSSLLWRKHGAQ"
     gene            1352..2569
                     /locus_tag="P5292_07175"
     CDS             1352..2569
                     /locus_tag="P5292_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HRS38947.1"
                     /translation="MTSPSNSKALSFIFITLLIDVIGLGIIIPVLPALIEQITGGDIS
                     EASRTGGWLTFAYAAMQFLFSPVIGGLSDRYGRRPVLLASLLGFGLDYILMGFAPTIG
                     WLFLGRLIAGITGASYTTAGAYIADVSTPEKRAQNFGLIGVAFGVGFIIGPVLGGLLG
                     HYSPRLPFFVAAGLALLNALYGYFVLPESLTPEHRRPFDWKRANPIGSLRHLGRYPVV
                     LSLVISFVLLYLSGHATQSTWTFYTIEKFGWNEQWVGYSLGFVGLMVAIVQGGLIRIV
                     IPKLGQKRSVFVGLTFYTIGFFLFAIATKGWMMFLFVVPFAMGGFAGPALQSIITSAV
                     PANEQGELQGALTSLISLTSIFGPLMMTNLFSVFSAPDAPVYFPGAPFMMGALLSLLS
                     IYFSMRTLTKHGH"
BASE COUNT          549 a          799 c          744 g          744 t
ORIGIN      
        1 gatcaatcgg gcagtgtttt ctcataagtc cgggcctgat ccgggtcatg gaatttactg
       61 agctccgtgc ttgcgccaga gtagggaaga gtccccataa ctcagaaacc gatacccgtt
      121 tgccaacgca tgatggtaca cggttcgcca gtccgaaccg atgaatgcag cgaccaatag
      181 caacaaagtg ctttcgggtt gatgaaaatt agtcaccagc ccatccacca cccggaagga
      241 ataccccggc gcgatcaata attgcgtatg gccggagatg gcagtgcggt tcgactcgtc
      301 catccggcga agtaaggccg taagcgcatc ttgtaccggt acgtcgtttt gtgattcata
      361 cggttcccat tgtccgatgc aaacttctgc ttccggcttt ccagcgagga cacgacaccc
      421 gagccagtat agactttcga gcgtgcgtaa actggtggtc cccaccgcaa tcacgcgtcc
      481 cgattcgtgc gcgtgaagta aacgctcgat catttcacga cgaacgatca ggcgttcccg
      541 gtgcatggga tgttcctgca tcgtcggcga cttcaccggc ttgaaggttc cggctccgac
      601 atgcagggtc agccaatcga ctccgatacc ttgttcgcgc agtttggaaa agactcgctc
      661 cgtaaaatgc aagccggcgg tcggcgcggc aactgaaccg gggagccgtg cgtaaacggt
      721 ctgataacgt tcagcgtctt cgggctcctc gtcgcggtcc atgtaaggtg gtagtggcat
      781 acggccgatc gcctccagaa tttcaccgaa cgtctccggg ctgttccaat tgaaacggac
      841 gatataggca tcatcctgct tgtcgccttt gatcgcctct aattgtattg ctcgtcccgc
      901 caattggagt tcccatacca gcggctcctc ggacttccag cgttttgcgt ttccgatcaa
      961 cgacagccat tcggcggttc cggtgacttg caacgcctgc gtcggatcga taccgttcgc
     1021 tggatcaagc acaagcaatt cgatccggct tccggttggt ttgcggtaga gcaggcgggc
     1081 gcgtattaca cgcgtatcgt tgaatacgag caggtcattc ggacttagga aatccggtaa
     1141 gtgttgaaac gatgtatcgg tgattttacc ttcctgatag acgagcaacc tggacgcatc
     1201 ccgctctgac agcggatgct gcgcgatccg gtcttcgggt aatgaatagg taaagtccaa
     1261 tatggaaatt ctgcgcggat ccatggtgtg agttgcaaag gtagcggttg ccggaaaagt
     1321 ccgtttcttt gcggcgccct tccccttgtc aatgacctcc ccttccaata gtaaagcgct
     1381 ttcattcatt ttcattaccc tcctgatcga tgtgatcggg ctcgggatca tcattccggt
     1441 cttgccagcc ctgattgaac agatcaccgg cggggatatc agcgaggctt cccgtacagg
     1501 cggttggctg acgttcgcct acgcggcgat gcagttcctt ttctctccgg taatcggtgg
     1561 cctgtcggat cgctacgggc gccgtccggt actgctggct tcgctgctcg gtttcggcct
     1621 tgactatatc ctgatgggat tcgcgcccac gatcggctgg ttgttcctcg gtcgcttgat
     1681 cgcgggtatc accggcgcga gctataccac tgcgggagct tacatcgcgg atgtcagcac
     1741 ccccgaaaag cgtgcgcaaa acttcggact gatcggtgtc gcttttggcg tgggtttcat
     1801 catcggaccg gtgcttggcg gtttgctcgg acattacagt ccgcgcctgc cgtttttcgt
     1861 cgcggcagga cttgcactgc tcaatgcgct ttacggctat ttcgttttac cggaatcttt
     1921 gacacctgaa caccgtcgtc ccttcgactg gaaacgtgcc aacccgattg gttctctccg
     1981 tcacctgggc cgttatcctg tcgtattgag tctggtcatt tcgtttgtac tcctctatct
     2041 gtcgggacac gctacccaaa gcacttggac cttttacacg atcgaaaagt tcggctggaa
     2101 cgaacaatgg gtcggttatt cgctgggttt tgtcggcttg atggtggcca ttgtccaggg
     2161 aggactcatc cggatcgtca taccgaaact cggacaaaag cgctcggttt tcgtgggcct
     2221 gacgttctat acgatcggct ttttcctgtt cgccatcgct acgaaagggt ggatgatgtt
     2281 cttgttcgtg gttcccttcg ccatgggcgg tttcgccggg ccggctttac agagtatcat
     2341 cacgtcggct gtaccggcga acgaacaggg cgaattacag ggagccctca ccagcctcat
     2401 cagcctgacg tccatcttcg gacccttgat gatgaccaat ctcttctccg tcttttccgc
     2461 ccccgatgcg cctgtctatt tccccggcgc cccgttcatg atgggcgcac tgctgtcact
     2521 gctcagcatc tacttctcga tgcggaccct gacgaagcat ggacactgac gtgtgttttt
     2581 ggtttcgagt tccgggttgg taattggtaa ttagtaatta gtaactaaaa attagtcttt
     2641 ctaattacta attactaatt accaattact agtttctaat tactagtttc cagtttctaa
     2701 tctctaatca ccaatcccca atcccccttt cctcgtccct cgtccctcgc agctcattcc
     2761 aacgccgtcc gcatcgcctg gatctcctgt tcgaggcgat cgaatttttc gcggaggtcg
     2821 tctttgcgga gttcgg
//
LOCUS       DAOTON010000327         2836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27627_length_2836_cov_3.164329, whole genome shotgun sequence.
ACCESSION   DAOTON010000327 DAOTON010000000
VERSION     DAOTON010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27627_length_2836_cov_3.164329"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /gene="uraD"
                     /locus_tag="P5292_07180"
     CDS             <1..415
                     /gene="uraD"
                     /locus_tag="P5292_07180"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HRS38948.1"
                     /translation="MLAARPFRDTASCLQVAEQAWSTTTASDWLEAFSNHPKIGDLDS
                     LTKKFASTSHLAGKEQAAVGNASQSTLQALAKGNDEYEKKFGFIFIVCATGKSADEML
                     DLLQQRLANDRTTELNIAAGEQLKITLLRLQKLIA"
     gene            412..753
                     /gene="uraH"
                     /locus_tag="P5292_07185"
     CDS             412..753
                     /gene="uraH"
                     /locus_tag="P5292_07185"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HRS38949.1"
                     /translation="MSQITTHVLDTATGKPASGIPVMLEARTGPDHWERVAQGETDND
                     GRIPGFVLPDRVLPPGTYRIRFETATYFQRTNVQGFYPWVEVVFDLRDSSHYHVPLLL
                     SPYGYSTYRGS"
     gene            776..982
                     /locus_tag="P5292_07190"
     CDS             776..982
                     /locus_tag="P5292_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38950.1"
                     /translation="MSERHKTVHDPWKWGWCYHNREDPRVWVPKKNPAAGFTLNFAHG
                     QAYAILLLLLMAPIMIVLLIELLK"
     gene            1001..2437
                     /locus_tag="P5292_07195"
     CDS             1001..2437
                     /locus_tag="P5292_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRS38951.1"
                     /translation="MKTTLDTNTRNAILDELRQANLAFQKVYPGDRPDRQPVHTVYGG
                     ANLFRYDSARRMGEIALRSLRTYAPDFTVLARVLGLTGHESLPTDATSIATLSKQLGE
                     MAPADRKKHPAWLPYTVYEKVIAKLEREALEDFRIDFEDGFGNRPDEEEDQTAIQAAA
                     EVAKGMRENSLPPFIGIRIKPFTEDLKARGVRTLDLFLTALLTETNGKLPDNFVVMLP
                     KVTIPEQIRALVACFKLIEAQFRMQEGSLKMEMMVEATQAIMDKDGRNPLRGLIMAGE
                     GRCIAAHFGTYDYTASCNITARYQTMDHPVCDFAHHMTKVALTGTGIFLSDGATNVMP
                     IGPHKGDNLTPEQHEENTRVVHRAWKLCFGHSTHSLVNGFYQGWDLNPAQLPMRYAAV
                     YTFFLESYEDAAIRLRHFVEKAAQATLSGDIFDDAATGQGLLNYFLRALNSGAISEEE
                     ALVTGLTLEEIRSRSFYRILQGRRSGRR"
     gene            complement(2548..>2836)
                     /locus_tag="P5292_07200"
     CDS             complement(2548..>2836)
                     /locus_tag="P5292_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRS38952.1"
                     /translation="FPKEEVFALTSQIRNSSRSVCANLSEGYRKRTYRAHFMSKLTDA
                     DMENSETGVWLDFAMACGYLNQEKYQALIRDNEAIGRLIGYMINHIDEFRR"
BASE COUNT          699 a          874 c          692 g          571 t
ORIGIN      
        1 catgctggct gcccgcccat tccgcgacac agccagttgt ttgcaggttg cggagcaggc
       61 atggtccacc acaacggcat ccgactggct ggaagcgttc tcgaatcatc cgaaaatcgg
      121 cgacctggat agcctgacaa aaaagttcgc atccaccagc cacctcgccg gaaaagaaca
      181 agctgctgtc ggcaacgcat cacagtcaac gcttcaagcg ctggccaaag gaaatgacga
      241 gtacgaaaag aagttcggct tcattttcat cgtctgcgct accggtaaaa gtgctgatga
      301 aatgcttgac ctgttgcagc aacggctagc gaacgaccga acaaccgaac tgaacatcgc
      361 cgcgggagaa caactcaaga tcaccttgct tcgactccaa aaactaatcg catgagtcag
      421 atcaccaccc acgtactcga taccgcgacc ggcaagccgg cttccggcat cccggtcatg
      481 ctcgaggcac gtaccggacc cgatcattgg gaacgcgtcg cgcagggaga aaccgacaac
      541 gacggccgta tcccaggatt cgtactaccc gaccgtgtcc tcccgcccgg aacgtatcgg
      601 atacgctttg aaaccgcgac ctatttccaa cgaaccaatg tacaaggctt ctatccatgg
      661 gtggaggtcg tattcgatct gcgggatagt tcacactatc acgttcccct cctgctcagt
      721 ccttacggat attctaccta tcgcggaagc tgaccatcat cctgcacatc ataacatgag
      781 cgaacggcac aaaacggttc atgatccctg gaaatggggc tggtgctatc acaaccgcga
      841 agatccccgc gtgtgggtac cgaaaaaaaa tcctgcagcc ggattcaccc tgaatttcgc
      901 acacgggcag gcctatgcca tcctgctact cctgctgatg gccccaataa tgatcgtact
      961 gctcatcgaa ctgctcaagt aatcccatcc tcccgctcgt atgaaaacca cccttgatac
     1021 caacacgcga aacgctattc tcgacgaact tcggcaggct aacctggctt ttcaaaaggt
     1081 gtaccccggc gaccggcccg accgtcaacc ggttcatacc gtctatggcg gcgccaacct
     1141 gttccgctac gactctgcac gccgcatggg cgagatcgca ctgcgcagcc tgagaaccta
     1201 tgcacccgat ttcaccgtgc tcgcacgtgt cctcggactt accggtcacg aatcactccc
     1261 gaccgacgcc accagcattg caacgctcag taaacagctg ggcgaaatgg cgcctgcaga
     1321 tcgaaagaaa caccccgcct ggctacccta cacggtctat gaaaaggtga ttgccaaact
     1381 cgaacgggaa gccctcgaag atttccgcat cgacttcgaa gacggcttcg gtaatcgccc
     1441 ggacgaagaa gaagaccaaa cggccatcca ggctgccgcg gaagtggcta aagggatgcg
     1501 cgaaaattcc ttgccgccct tcatcggcat ccgcatcaaa ccgttcaccg aagacctcaa
     1561 agcgcggggt gtacgcaccc tcgacctgtt cctcaccgca ctcctcacgg aaaccaacgg
     1621 taaactgccc gacaactttg tcgtcatgct cccgaaggtg accatcccgg aacagatccg
     1681 cgccctggtg gcatgcttca aactcatcga agcacaattc cggatgcagg aaggcagcct
     1741 caagatggaa atgatggtgg aggcgacgca ggcaatcatg gacaaagacg gccgcaaccc
     1801 cttgcgcgga ctcatcatgg ccggagaagg acgttgtatc gccgcccact tcggcaccta
     1861 cgactacacc gcttcctgca acatcaccgc acgttatcag acaatggacc atccggtttg
     1921 cgatttcgcg catcacatga cgaaagtcgc cctcaccggc accggcatct ttctctctga
     1981 tggagccacg aatgtcatgc cgatcggccc gcacaaagga gataatctga cgcccgaaca
     2041 gcacgaagag aatacccgcg ttgtgcaccg cgcctggaag ctctgctttg gtcactccac
     2101 ccattcattg gtgaatggct tttaccaggg atgggacctc aaccccgcac aactcccgat
     2161 gcgctacgcc gccgtttaca ccttcttcct cgaaagctac gaagacgctg ccatccggct
     2221 tcggcacttc gtcgaaaagg cggctcaagc caccctgtcc ggcgacatct tcgacgatgc
     2281 agcaaccgga caaggcctgc tcaactactt cctgcgtgcc ctgaactccg gcgccatcag
     2341 cgaagaagaa gccctcgtca ccggcctaac cctcgaagaa atccgcagcc gctcgttcta
     2401 tcggatcctg caagggagaa ggagcgggag gcggtaggcg gtaggcggta ggcagtgggc
     2461 agtggacagt gggcagtggg cggtgggcgg tgactagggg ttgtcgttcg tttggttgat
     2521 gcttgggttg aatcaatttt ccagaactca tcttctgaat tcgtcgatat gattgatcat
     2581 gtagccaatc aatctgccta tggcttcatt atcacgaatt aatgcctgat acttttcctg
     2641 gttcagatat ccgcaagcca ttgcgaaatc caaccaaaca ccggtttcag agttctccat
     2701 gtcagcatca gtaagctttg acataaagtg cgccctataa gttctttttc tgtacccttc
     2761 cgacaaattt gcgcagacag atcttgaaga gttcctgatt tgactcgtta aagcaaaaac
     2821 ctcttctttc ggaaat
//
LOCUS       DAOTON010000328         2831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27681_length_2831_cov_3.663184, whole genome shotgun sequence.
ACCESSION   DAOTON010000328 DAOTON010000000
VERSION     DAOTON010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27681_length_2831_cov_3.663184"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1830
                     /gene="pckA"
                     /locus_tag="P5292_07205"
     CDS             214..1830
                     /gene="pckA"
                     /locus_tag="P5292_07205"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRS38953.1"
                     /translation="MNEFGLKAKDATIADLGLRNVADAYWNLTPAELVEETIIRGEGM
                     LTDTGALAVDTGKFTGRSPKDKFVVCDAKTENSVWWGDINIKFDPDKFDRLYEKVAGY
                     LQGKTIYVRDAYACADPKHRLNIRVVTESPWQNLFAYNLFLRPTKEEILQTVKPEWTI
                     VAAPGFEADPKLDGTRQSNFAIVNFSKKIILIGGTGYTGEIKKGIFSVLNYILPHEKN
                     VLSMHCSANIGKNGDTAIFFGLSGTGKTTLSADPNRGLIGDDEHGWGDTSVFNFEGGC
                     YAKCVNLSKEKEPQIFNAIRFGALVENIEFYEGTTTVDYDNISKTENTRAAYPIHHID
                     NAVEPSVAGTPKNIFFLTCDAFGVLPPISKLDAGQAMYHFISGYTAKVAGTEVGITEP
                     TTTFSACFGKAFLPLHPTRYAELLGEKLKKNKVNVWLVNTGWSGGSYGVGSRIKLSFT
                     RALITAALNGELDKVEYDTMPVFGLSMPKTCPGVPTEILNPRNTWNDKNEFDAKANTL
                     AASFVKNFAQYAEYANAEILAAAPKLAVNA"
     gene            1962..2327
                     /locus_tag="P5292_07210"
     CDS             1962..2327
                     /locus_tag="P5292_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38954.1"
                     /translation="MNHSSAKVHTHTAMVTILELEDGDELAGSWSTPDHDRFGTFRLL
                     LKRTPDNRLLWAHYIQAPDGTTGMVMRGELRHPDELKQLQDTIHRTIRKVTGEELRLE
                     DSDYLASSADGISLRPVQE"
     gene            2332..>2831
                     /locus_tag="P5292_07215"
     CDS             2332..>2831
                     /locus_tag="P5292_07215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRS38955.1"
                     /translation="MELFFCNAIDDNPVMLGPDESWHLSKVLRLRVGDAVAVTDGHGG
                     FGTGEIMDINPKAALIRLKEVRQAYNQRPYSLHIAIAPTKNIDRLEWFLEKATEIGIA
                     AISPIRCQRSERKDVRIDRLEKVVMAAVKQSLQAYLPQIHPMESLSKFLESNPAGQRF
                     ACITHQT"
BASE COUNT          707 a          801 c          719 g          604 t
ORIGIN      
        1 ggaaggcgaa accgattttt gcctcggttt ggcagaactt agtcaatccg aacaagatca
       61 attcattgcg cgatcccggg agaaattacg ggacgctaag tgggtcaatc tctacgagaa
      121 cagtccctgc aggcaccgcc gggccaagta attggccaaa ccggcctttt ttactacatt
      181 tgcgccatct ttcagttacc aaatcacctg atcatgaacg aattcggatt gaaagccaag
      241 gatgccacga ttgctgatct tggccttcgt aacgttgctg acgcctattg gaacctgact
      301 ccggccgaac tggtcgaaga aacgatcatc cgaggtgaag gcatgctgac cgacaccggt
      361 gcgcttgcag tcgataccgg caaattcact ggccgttccc ccaaagacaa atttgtggtc
      421 tgtgacgcca agacggaaaa ctccgtatgg tggggcgaca tcaacatcaa attcgaccct
      481 gataaattcg atcgtcttta cgagaaagtt gcaggatacc ttcagggtaa aaccatttat
      541 gtacgcgacg cttatgcctg tgctgatccg aagcaccgcc tgaacatccg ggtcgtgacc
      601 gaatcaccct ggcaaaacct gtttgcgtat aacttgttcc tccgtccgac gaaagaggag
      661 atcctacaaa cggtaaaacc ggagtggacc atcgtcgcgg ctcccggctt cgaagcggac
      721 ccaaaactgg acggaacgcg tcaaagcaat ttcgccatcg tcaacttcag caagaagatc
      781 atcctaatcg gcggcaccgg gtacacaggc gagatcaaga agggtatctt ctcggtactc
      841 aactacatcc ttccgcacga gaagaatgta ctctccatgc actgctccgc caacatcggg
      901 aagaacggcg acaccgcgat cttcttcggt ctctccggca ccggcaagac taccttgtcc
      961 gctgatccga accgcggcct catcggcgac gacgaacacg gctggggcga cacttccgtc
     1021 ttcaacttcg aaggcggttg ctatgccaag tgcgtcaacc tctcgaagga gaaagaacct
     1081 cagatcttca acgcgatccg tttcggcgca ctcgtggaga acatcgagtt ctatgaaggg
     1141 acaacgacgg ttgattacga caacatcagc aagacggaga acacccgcgc tgcctatcct
     1201 atccatcata tcgacaacgc ggtcgagcct tcggtggccg gtactccgaa gaacatcttc
     1261 ttccttacct gcgacgcttt cggcgtactt cctccgattt cgaagttgga tgccggtcag
     1321 gcgatgtatc acttcatttc cggatatacc gccaaggttg ccggtacgga agtgggcatc
     1381 accgagccga ccaccacctt ctccgcctgc tttggcaaag ccttcctacc gcttcacccg
     1441 acccgctacg cggagttgtt gggcgaaaaa ttgaagaaga acaaagtaaa cgtctggttg
     1501 gtcaacaccg gctggtcggg cggatcgtat ggcgtgggat cccgcattaa actgtcgttc
     1561 acccgtgcct tgatcactgc tgccctgaac ggagaactcg ataaagtcga gtacgatacc
     1621 atgccggttt tcggactttc catgccgaaa acgtgtcctg gcgtaccgac ggagatcctg
     1681 aatccacgga atacctggaa cgataagaac gagtttgatg cgaaggcaaa tacgcttgcc
     1741 gcctcgtttg tcaagaactt cgcacaatat gctgagtacg cgaatgcaga gattctggca
     1801 gcagcaccga aactagctgt caacgcctga ctgctcaact gattcgacaa gaagccgccc
     1861 tcaacagggc ggcttttttt tgcggtcgat tttttcatcc agcatataat ggttggacga
     1921 ctacccggac gtaggtgaat ttagccgctc attcgacaac catgaatcat tcatccgcta
     1981 aagtccacac ccatacggcc atggtcacca tcctggaact tgaggacgga gacgaactgg
     2041 ccggatcctg gagcacgcct gatcatgacc ggttcgggac tttccggttg ctgcttaaac
     2101 gcacacctga caaccgattg ctttgggcac attacatcca ggcaccggac ggaacaaccg
     2161 gcatggtcat gcgtggagag cttcggcatc ctgacgagct gaagcaattg caggatacga
     2221 tccaccggac catccggaaa gtcaccgggg aggaattacg tttggaagac tccgactatc
     2281 ttgcgtcttc cgccgacggg atctccctcc gacccgtaca ggaataactg catggaactt
     2341 ttcttctgca acgcgatcga cgacaatccg gttatgctgg gtccggacga atcctggcac
     2401 ctttccaagg tgttgcggtt gcgtgttgga gatgcggtag ccgtcaccga cgggcacgga
     2461 ggattcggga ccggggaaat aatggatatc aatcccaagg cagcgctgat tcgtttgaaa
     2521 gaagtcagac aggcctacaa ccaacgcccc tactccctgc acatcgcgat cgcccccaca
     2581 aaaaacatcg accggttgga gtggtttctg gaaaaagcga ccgagatcgg catagctgcc
     2641 atcagtccga tccgttgcca gcgctccgag cgtaaggatg tccgcatcga tcggctggaa
     2701 aaagtcgtaa tggctgcagt gaagcaatcg ctgcaagcct acttgccgca gatacatccg
     2761 atggaaagtc tttcaaaatt cctggaatcc aaccctgcgg gtcaacgatt cgcctgcatt
     2821 acacatcaga c
//
LOCUS       DAOTON010000329         2830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27699_length_2830_cov_3.727928, whole genome shotgun sequence.
ACCESSION   DAOTON010000329 DAOTON010000000
VERSION     DAOTON010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27699_length_2830_cov_3.727928"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="P5292_07220"
     CDS             complement(<1..541)
                     /locus_tag="P5292_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRS38956.1"
                     /translation="MNDRSRRYVAIWLLSGCFLIFAMVVIGGITRLTGSGLSITEWAP
                     IMGAVPPMNGQEWQEAFEKYKQIPQYEMINAHFELQDFKAIFWWEFIHRLVGRLIGVV
                     FLLPFIWFLITRQLDRAMTRKALFLFTLGGLQGFLGWFMVKSGLTERTSVSHIRLAIH
                     LITAFITFGFTWWYALQLLY"
     gene            complement(597..2090)
                     /locus_tag="P5292_07225"
     CDS             complement(597..2090)
                     /locus_tag="P5292_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38957.1"
                     /translation="MTRTAKSGILLLAALFLCVFSARSQNATSSPYSRYGIGDLQFQG
                     GARHLGMGGVSIGVPLNLQLNFQNPAAYSNLFLTTFELGANANLMEINSADARQEQHT
                     VSFSYFALGVPLKSKRWGLGFGLMPYSNIGYSVKDPQTNVLGEVETRTYQGSGGLNNF
                     FISTGVRPLKNTPLSIGANVSYLFGVLGQDRRIEFDNPVNFNTRLLENTSLGWFTYNL
                     GVQYTIDSLRLSPSDSLLEFDKLIRPLEDSLVHLDDVARKASDSLQAGYAERREFLKN
                     RIRQLRQERGDVKRRRERGDWSLTFGATYSPSAELHARQTYTATSFKYFDNSSRDQIV
                     VRDTIAYVEGAEGRVKLPVGLGFGASIRQGTRWLAGADFQTQNWNTYRYFGASDSLAD
                     SWRVSVGGQFTPNDRVLKNRYKTVQYRAGFHYARTFLQLRGNQLNEMGVSIGLGIPIR
                     RASTFIQFAAEAGERGTVESDLLKERYLRFTLGFTISDRWFLKPKYD"
     gene            complement(2074..>2830)
                     /locus_tag="P5292_07230"
     CDS             complement(2074..>2830)
                     /locus_tag="P5292_07230"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRS38958.1"
                     /translation="YLDRMKSKGLLLAIDRGNTRVKIGVFSGGTLIYQEAFSVLSLNR
                     LQSLSRRFEFSGAILSAVAPLPVPVLKFLKKLPGFLELTHRTPLPIRNAYKSPATLGR
                     DRIAMAVAAVVRYPRQHVLVIGAGTCITFDLVQAGGTYMGGSISPGLHMRFEALHAFT
                     GGLPKVKLQWDNPLLGIDTLSSMRSGVMNGAMAEIREMVNEYKKMYPRLKVLITGGDA
                     AGLAERLKINIFAAPDLVLYGLYEIYRYHDANR"
BASE COUNT          705 a          772 c          800 g          553 t
ORIGIN      
        1 gatacagcaa ctgcaacgcg taccaccagg tgaagccgaa ggtgatgaag gccgtgatca
       61 ggtgaatggc cagccggata tgcgacacac tcgtacgttc ggtgagcccg ctcttcacca
      121 tgaaccagcc aaggaacccc tgcaagccac cgagtgtgaa caggaagagc gccttccggg
      181 tcatggcgcg atccagttga cgggtgatca ggaaccagat gaacggcagc agaaacacca
      241 caccgatcaa cctgccgacc aggcggtgaa tgaactccca ccagaagatc gccttgaaat
      301 cctgcaattc gaaatgcgca ttgatcatct cgtactgggg aatctgtttg tacttctcaa
      361 aagcttcctg ccactcctgg ccgttcatag gaggtacagc gcccatgatc ggagcccatt
      421 ccgtgatgga taagccggaa ccggtcaggc gggtgatgcc gccgatgacc accatcgcga
      481 agatcaggaa acagccgctc agcagccaga ttgcgacgta acgacgagat cgatcgttca
      541 tatgctttgc agctataacc attccggtac ggcaaagttc aaatcgaagc aggagatcag
      601 tcgtacttcg gcttgaggaa ccagcggtcg gaaatggtaa agcccagggt gaaacgcagg
      661 tagcgttcct tgagcaggtc ggattcgacc gtaccgcgtt cgcctgcctc cgcagcgaac
      721 tggatgaatg tggaagcacg ccggatcgga attccgagtc cgatgctcac acccatttcg
      781 ttcaactggt taccgcgcaa ttggagaaat gtacgggcat agtggaatcc ggcacggtac
      841 tgaacggtct tatagcggtt tttcaagacg cgatcattcg gagtgaactg cccgccgacg
      901 gaaacgcgcc agctatcggc cagtgaatcg gacgcaccga agtaacgata ggtattccag
      961 ttctgcgtct ggaagtcggc accggccaac cagcgcgtac cctgccggat agaagcgccg
     1021 aagccaaggc cgacaggaag tttgacacgc ccctctgctc cttcaacata agcgatcgta
     1081 tcccgcacga caatctggtc gcgcgaggaa ttgtcgaagt acttgaacga agtggccgta
     1141 tacgtctgcc gggcatgcaa ttcggcggaa ggcgaatacg tcgcgccgaa cgtcaggctc
     1201 caatcgcccc gttcacgccg acgcttcacg tctccccgtt cctgacggag ttgccgaatg
     1261 cggtttttca ggaattcgcg acgctccgca taaccggctt gcaggctgtc ggacgctttg
     1321 cgcgcaacat cgtccaggtg cacgagcgaa tcctccaacg gacgaatgag cttgtcgaac
     1381 tccagcaagg aatcagacgg agagagccgc aggctatcga tggtgtattg aacaccgagg
     1441 ttataggtaa accagccgag cgatgtattc tccagtaaac gcgtgttgaa gttcaccgga
     1501 ttgtcgaatt cgatccgtcg gtcctgaccg agcacaccga agaggtacga gacattcgcg
     1561 ccgatactca gcggcgtatt cttgagcgga cgaacgcccg tagaaatgaa aaagttgttg
     1621 agcccgccgc ttccctggta agtacgtgtc tccacttcac cgagcacatt ggtctgcgga
     1681 tctttaacgg agtacccgat gttgctgtag ggcatgagtc cgaaaccgag tccccaccgc
     1741 ttcgacttga gcggcactcc gagcgcgaag tagctgaagg aaaccgtatg ctgttcctgt
     1801 cgggcgtcgg ctgaattgat ctccatcaaa ttggcattgg cgcccaactc gaaggtggtc
     1861 aggaagaggt tggaataagc cgcggggttc tgaaagttca gttgaaggtt gagcggaaca
     1921 ccgatggaaa cgccccccat cccgaggtga cgagcaccgc cctggaattg caggtcgccg
     1981 atgccatacc gggagtaagg cgaggaagta gcattctgag aacgcgcgga aaacacgcac
     2041 aggaaaagcg ccgccagcag taagatgccg gacttagcgg ttcgcgtcat ggtaacgata
     2101 gatttcataa agtccgtaga ggaccaaatc aggggcggca aagatgttga tttttagccg
     2161 ttcagccaaa ccggccgcgt ctccgccggt gatcaggact ttcagccgcg gatacatctt
     2221 tttgtactcg ttgaccattt cacggatctc agccattgcc ccattcatga cgcccgaacg
     2281 catactggaa agcgtgtcaa tgccaagcaa gggattatcc cattgcagct tcactttcgg
     2341 cagacctccg gtaaaggcat ggagtgcttc aaaacgcatg tgaaggccgg gagaaatact
     2401 gccgcccata taggtgccac ccgcttgaac caggtcaaag gtgatacagg tcccagctcc
     2461 gattaccaat acgtgctgac gggggtagcg gaccaccgct gccacggcca tcgcgatccg
     2521 gtcgcgtccc agcgtagccg gcgatttgta agcattccgg atagggagtg gagtacgatg
     2581 agtgagttca aggaaaccgg gcaacttttt taagaatttt aataccggaa caggcagtgg
     2641 tgcgacggca gataatatgg caccggaaaa ttcgaaacga cgcgagagcg attgcaagcg
     2701 gttcaggctc agcacagaga atgcctcctg gtagatcaac gtaccgcccg aaaagacacc
     2761 gattttcacc cgggtgttac cgcggtcgat ggccagcaaa agacctttgc ttttcatgcg
     2821 gtcaagatac
//
LOCUS       DAOTON010000330         2827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27742_length_2827_cov_2.151515, whole genome shotgun sequence.
ACCESSION   DAOTON010000330 DAOTON010000000
VERSION     DAOTON010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27742_length_2827_cov_2.151515"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="P5292_07235"
     CDS             complement(<1..182)
                     /locus_tag="P5292_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38959.1"
                     /translation="MDLRSFFPLSLRLLLVRIRWFWKHYRSIRYNSELFNNVHGPLTY
                     NTDGLVTSNNADFMRE"
     gene            complement(184..738)
                     /locus_tag="P5292_07240"
     CDS             complement(184..738)
                     /locus_tag="P5292_07240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015857433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRS38960.1"
                     /translation="MLGRLLRLFRKPQPVQPVDAGAGSEIKGTVVRLEEGSVIRVGAG
                     CLVEGILSTYTANARITLGDNVFVGNNTLIGSACEVTIGNNVLISFDCMIQDNDNHNT
                     DSKIRWNDTAEWKNGRRHDWSKTPMQPIRIGNGAWIGAKAIILKGVTIGEGAIVGAGS
                     VVTRDVAPYTIVAGNPARFIRNTE"
     gene            complement(742..1449)
                     /locus_tag="P5292_07245"
     CDS             complement(742..1449)
                     /locus_tag="P5292_07245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS38961.1"
                     /translation="MITRILQLLTPPVIPAIGRRLGILSTPGKIIRYPKNENDTQDLD
                     MYFDPEYAKILDNWGADNTWIEIQMLLCNCEGKVLDIACGTGVTISILGKYPKLEVHG
                     CDISDLLIAKAAERGIPKDRLAVTDATQMSYPNDSFDYSYSIGSLEHFTTNGIDKFIA
                     EASRITRKCSMHMIPTSKSGQNEGWMTTSTTIQTFHNNSDAWWLERFKRHFREVHILN
                     SRWNDGISFGRWFVCKR"
     gene            complement(1484..2662)
                     /locus_tag="P5292_07250"
     CDS             complement(1484..2662)
                     /locus_tag="P5292_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HRS38962.1"
                     /translation="MSKLPILEVRDVSKRFDIRHSSGQYLTFREQLLQLVSLKGSGKK
                     EDFWALKNVSFDIQPGESVGIVGKNGAGKSTLLKILSKITPPTTGLIRSRGRIASLLE
                     VGTGFHPELTGRENIYLNGSILGMLRSEINARFDDIVEFSGVAQFLDTPLKHYSSGMQ
                     LRLALAVGDSEFQSKCLKKMDEISHDEERTILFVSHNLGLVTQLCSRCIWLKEGSVHR
                     NGATETIVQEYLQSSASGTGTYVSTPEHEAHKDVCFQEIYAGTRDGRPNDIFGFDDSI
                     HLFIRVKDRSRQKNMKIGVSLADRFGNFIFTILEHADRFRTSNADHILIRLCLPSSLI
                     APGAYSFRYALFIDNGKVYEVLENETPVRIVDTGSSMAAYEGVNYGSIIVHPEWDTWQ
                     "
BASE COUNT          636 a          705 c          783 g          703 t
ORIGIN      
        1 tcctcacgca tgaaatcggc attgttactg gtcacaagac catcggtgtt gtaggtcaat
       61 ggaccatgta cgttgttgaa caattcgctg ttataacgaa tcgagcgata gtgtttccag
      121 aaccagcgga tacggaccag caacaatcgc aacgataagg ggaaaaagga tcggaggtcc
      181 attttattcg gtattacgga tgaaccgggc cggattgccg gcaacgatgg tatagggcgc
      241 gacgtcgcgg gtcaccacac tgccagcgcc gacaatggct ccttcaccaa tggtgacgcc
      301 cttcaggatg atcgcttttg caccgatcca ggcgccattg ccgattcgaa tgggttgcat
      361 tggcgtcttt gaccagtcgt gtcgccgacc gtttttccat tcggcggtat cattccagcg
      421 gatcttcgaa tcggtattgt ggttgtcgtt gtcctggatc atgcagtcga acgagatcag
      481 tacattgtta ccaatggtga cttcgcaagc ggatccgatc aatgtgttgt tgccgacaaa
      541 gacattatcg ccaagcgtga tacgggcatt ggcggtatag gtgctcagta tgccttcgac
      601 caggcagccg gccccgaccc ggatcaccga tccttcttcg aggcgcacaa cggtgccctt
      661 gatctccgaa cctgcaccgg catcaacggg ttgcaccggc tgcggttttc gaaacagacg
      721 taggagacga cccagcattg ctcagcgttt acagacgaac caccttccga aggatatgcc
      781 gtcgttccaa cgggaattaa ggatatgaac ttcgcggaag tgacgcttga atcgctccag
      841 ccaccacgca tcggaattgt tgtgaaaagt ctggatcgtg gtagaggtgg tcatccagcc
      901 ttcattctgt ccgcttttgg atgtcgggat catgtgcatg gaacatttgc gcgtgatccg
      961 cgatgcttcc gcgatgaact tgtcaatccc attcgtcgtg aaatgttcca gagatcctat
     1021 actgtaagaa tagtcaaagc tgtcgttcgg ataactcatc tgcgtcgcgt ccgtgaccgc
     1081 taaacgatct ttcggaatgc cgcgttcggc tgctttggcg atgagcaggt cggaaatgtc
     1141 gcagccgtgc acttccagtt tcgggtactt gccaagaatc gagatggtca cgcccgtgcc
     1201 gcaggcgatg tcgagcactt tcccttcgca gttacataac aacatctgga tctcgatcca
     1261 tgtattgtct gcgccccagt tgtcgaggat cttggcgtat tcggggtcga aatacatatc
     1321 aaggtcctga gtatcgttct cgtttttcgg atagcggatg atctttcccg gtgtggacag
     1381 aatgccgaga cgacgaccga ttgcgggaat gaccggcggt gttagcagtt gaagtattct
     1441 ggtgatcatg tggatcgaag ttaaaacagc gtataagtta cgtttattgc caggtatccc
     1501 actcagggtg aacgatgatg ctgccgtaat tgacgccttc gtatgccgcc attgaggatc
     1561 cggtatcgac gatgcgcacc ggcgtttcgt tttcgaggac ttcgtacacc ttaccgttgt
     1621 cgatgaacaa ggcgtaccgg aaggaatagg ctccgggcgc gatcagggag gatggaagac
     1681 aaagtcggat caggatatga tcggcattgc tggtcctgaa cctgtcggca tgttcgagta
     1741 tcgtaaagat gaagtttccg aagcggtcgg caagcgagac gccgatcttc atattctttt
     1801 gacgggaacg gtctttgact ctgatgaata ggtggatgga gtcgtcgaag ccgaaaatgt
     1861 cgttcggccg accgtcgcga gtgcctgcat agatctcttg aaagcataca tccttgtgtg
     1921 cttcgtgttc aggcgtgctg acgtaggtgc cggttccgga ggccgatgat tgaaggtatt
     1981 cctgaacgat cgtttctgta gcgccgtttc ggtgtaccga tccttctttc agccagatac
     2041 agcgggagca caattgtgtg acaaggccga gattatgact gacgaagaga atcgttcgct
     2101 cttcatcatg actgatctcg tccattttct tcaggcactt gctttgaaat tccgaatccc
     2161 cgacggccaa agccagccgc agttgcattc cactcgagta atgcttcagg ggtgtgtcca
     2221 ggaactgcgc gaccccgctg aattcaacga tgtcgtcgaa ccgtgcgttg atctcggaac
     2281 gcagcatgcc taagatggaa ccgttgaggt aaatattctc tcggccagtc agctcgggat
     2341 gaaaaccggt tcccacttcc aacaaactgg cgattcgtcc gcgcgatcgg atcagcccgg
     2401 tggtgggtgg ggtgatcttg gagagtatct tcagcagggt cgactttccg gctccgtttt
     2461 tgccgactat cccgaccgat tcgccgggtt gtatgtcgaa cgagacgttt ttcaacgccc
     2521 agaaatcttc cttctttcca ctgcctttga gactaaccaa ttgcaatagc tgctcccgga
     2581 aggtcagata ttgacccgac gaatggcgta tgtcgaaacg tttcgataca tcgcgtactt
     2641 cgagaatggg cagcttggac atcaggagag gtcggcgaag tagttttctg tttttctgaa
     2701 gtaaagcaaa ccggtaatga atagcagaag ggcgccggtc acgctgatca ggcaaatgcc
     2761 tgcatccggc gtgctcccgt caaaggctga acgaaagatg gcgatcgcac catagagcgg
     2821 attgcac
//
LOCUS       DAOTON010000331         2826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27758_length_2826_cov_2.802959, whole genome shotgun sequence.
ACCESSION   DAOTON010000331 DAOTON010000000
VERSION     DAOTON010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27758_length_2826_cov_2.802959"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1310)
                     /locus_tag="P5292_07255"
     CDS             complement(51..1310)
                     /locus_tag="P5292_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS38963.1"
                     /translation="MRNPIWKIATTHLLAKKRQTLVATLGVTFGIMIFIFQAGLITGF
                     QAVFIEETVNSTANIHIFNEPDKDRPSIIQRVHRTDPERWIVVRNQKPKDELNKIRNG
                     FQIIEALERHPDVSGVSPFLGAQAILRAGIVQYSGRLAGVDIEKEDINFRVSDYMVQG
                     EVSRLKSTNNGIILGDGLADKLGVKMNDNLTVLTPNGNSLEMKVVGINHTGLTEVDKN
                     RAYISIRNAQRLLEVDNSYITDINIKLKNIDKAEALAREFERKFGYKAEDWKTANASI
                     FSVFKIQNMVTFLIIASILIVSGFGIFNILMMIIYEKMPDIAILKAIGYKDRDIRRLF
                     LIESLVIGVIGGLMGLIMGFLLSRFVGSIRMDVKGFVSMEYLRFNDSPGFYVFAFIFA
                     LIATSLAGYFPARKASKVDPIDIIRSK"
     gene            complement(1325..2407)
                     /locus_tag="P5292_07260"
     CDS             complement(1325..2407)
                     /locus_tag="P5292_07260"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRS38964.1"
                     /translation="MKQLLNLTLIILLAGCNRSGQETQPVRKEITQAVYASGKLYPIG
                     HYTVFSKIPGYVERVFVTAGQSVHRGDPLVSVRNETNEMNLESARNLVELAKLQASQH
                     GPVLSGLDQEVRAAKARYLLDSTNATRTASLWQQQATSRQQLDQAQTQFELSKSQYQR
                     AKENYTSNRERLEVEYRNAVNQLEALTANRSEYRILSVLDGKVYDVIPEVGDLVNAQT
                     PLVEIGDSSRFEVELAVDETDIALLHTGQPVVYAIDAYRDSVFHGVISEIIPRVNVLT
                     KSSRVKATFNLSPGVQLYTGMSAEANILISEKKNALVIPKEFVRNGNQVRLKGEDQPR
                     TIRKGIEDLQFIEVLEGLSEQDILVK"
     gene            complement(2462..>2826)
                     /locus_tag="P5292_07265"
     CDS             complement(2462..>2826)
                     /locus_tag="P5292_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AcrB/AcrD/AcrF family protein"
                     /protein_id="HRS38965.1"
                     /translation="TFDAIVEAGKTRMTPVILTAFATILGLIPLAVGLNIDFSRMFSE
                     FNPHIYFGGDNTAFWGPLSWTMIFGLGFGTFLTLIMVPVMYLLSIRMKNQLRKWRKQP
                     IDNGVAPKPVEELIEAHI"
BASE COUNT          612 a          731 c          762 g          721 t
ORIGIN      
        1 cgatttgcag agtcgccggg tctcgagaat atacggagtt acggccacca tcatttggaa
       61 cggatgatat cgatcgggtc cactttcgac gctttccggg cgggaaaata accggcgagt
      121 gaagtggcga tcagcgcgaa gatgaacgcg aatacataga atccggggga atcgttgaac
      181 cggagatatt ccatcgagac gaaacccttg acatccattc gtatactgcc gacgaagcgg
      241 gagagcagga agcccatgat caaccccatc aaaccaccga tgacgccaat cacgagcgat
      301 tcgatcagga acaatcggcg tatgtcccgg tccttgtaac cgatggcttt caggatagcg
      361 atgtccggca tcttctcata gatgatcatc atcagaatat taaatatgcc gaaacccgat
      421 acgatcagaa tggaggcgat gatcaggaaa gtgaccatgt tctggatctt gaataccgag
      481 aagatgctgg cattcgcggt tttccagtct tccgccttgt agccgaactt gcgttcgaat
      541 tcccgggcaa gcgcttccgc tttatcgatg ttctttaatt tgatgttgat gtccgtgata
      601 taggaattgt cgacctccag caaccgttgc gcattccgga tgctgatata tgcccggttc
      661 ttgtcgactt ccgtgagtcc ggtgtggttg atgccaacga ctttcatctc cagcgaattc
      721 ccgttcggcg tcagtacggt taggttgtcg ttcatcttta cgccaagctt gtcggccaga
      781 ccgtctccga ggatgatgcc gttgttggtc gacttcaaac ggctgacttc gccctggacc
      841 atgtaatcgc taacccgaaa attgatgtct tccttttcaa tatcgactcc ggcaagccgg
      901 ccactgtatt gcacgatgcc tgcacgcagg atggcttgag cgccgaggaa gggggagacc
      961 ccggatacgt cgggatgtcg ttccaatgct tcgatgatct gaaacccatt ccggatcttg
     1021 ttgagttcgt ccttgggctt ctggtttcgg acgacgatcc atcgttccgg atcggttctg
     1081 tgcacccgct ggatgatgct cggtcggtcc ttatccggct cattgaatat gtgaatgttg
     1141 gcggtcgagt tgaccgtctc ttcgatgaat acggcttgaa aaccggtgat cagccccgcc
     1201 tgaaaaatga agatcatgat cccgaaggtc acgccaagcg ttgcgaccag cgtctggcgc
     1261 tttttcgcaa gcaggtgcgt ggtagcgatc ttccagatgg ggtttcgcat acgagtccgg
     1321 atcgttattt gactaggata tcttgctccg aaagaccctc gaggacttcg atgaattgca
     1381 ggtcttcaat gcccttacgg atggtcctgg gttggtcttc gcctttcagc cgaacctggt
     1441 ttccgttgcg cacgaattct ttcggtatga ccagtgcatt cttcttttcg gaaatgagga
     1501 tgttggcttc cgccgacatg cccgtatata attggacccc cggtgaaaga ttgaaagtcg
     1561 ctttaacacg gctgctcttc gtcagcacgt tgacccgcgg aatgatttca gagatgacac
     1621 cgtggaatac ggaatcccga tatgcgtcaa tggcatatac aaccggttgc ccggtatgca
     1681 ataacgcgat atcggtttca tcaacggcaa gttcgacttc gaagcgcgaa gagtcaccga
     1741 tctcaaccag gggcgtctgg gcgttgacca ggtcgccaac ctctggaatg acatcatata
     1801 ccttaccgtc aaggaccgac aggatacggt actccgatcg atttgcggtc agcgcctcca
     1861 gctgattcac ggcgtttcgg tattcgactt ccagtcgctc ccggttgctc gtataattct
     1921 cctttgcgcg ttggtactga ctttttgaaa gctcgaattg tgtctgtgcc tggtccagtt
     1981 gctgccgact cgtggcttgt tgttgccaca aggatgctgt gcgtgtggca ttcgtactgt
     2041 cgagtagata gcgtgccttt gccgcacgta cctcctggtc cagtcctgaa agcaccgggc
     2101 catgctggct ggcctgcaac tttgcaagct ccaccaggtt gcgggcgctc tccaggttca
     2161 tctcattggt ttcgttacga accgaaacca atggatctcc gcgatgcacc gattggccgg
     2221 ctgtgacaaa tacgcgttca acatatcccg gtattttgct gaagactgtg tagtgaccaa
     2281 tcgggtagag cttccccgaa gcataaaccg cctgggtgat ttccttccga accggttgtg
     2341 tctcctgtcc gcttcggtta caccctgcca gcagaatgat cagtgtcaga ttgagcagtt
     2401 gtttcatggg atgttgaaga aacggccgga cgcgtgtcgc gaccggccgt gtaggttatc
     2461 atcagatatg tgcttcgatc agctcttcaa ccggcttcgg cgctacaccg ttgtcgatcg
     2521 gctgttttct ccacttgcgt aactggtttt tcatccggat gctgagcagg tacatgaccg
     2581 gtaccatgat cagggtcagg aaggtgccaa aaccgagtcc gaagatcatc gtccacgaca
     2641 gcgggcccca gaaagcggtg ttatcgcctc cgaagtagat gtgaggattg aattcggaga
     2701 acatccggga gaagtcgatg ttcaatccga ctgccaatgg gatcaatccg agaatcgtgg
     2761 cgaaagcggt caggataacc ggtgtcatac gggttttgcc ggcttccacg atggcatcaa
     2821 aggtcg
//
LOCUS       DAOTON010000332         2822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27811_length_2822_cov_3.577521, whole genome shotgun sequence.
ACCESSION   DAOTON010000332 DAOTON010000000
VERSION     DAOTON010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27811_length_2822_cov_3.577521"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="P5292_07270"
     CDS             complement(<1..180)
                     /locus_tag="P5292_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS38966.1"
                     /translation="MSRFRIFLRLFRESFLFAFEALRVNKLRTLLSLLGITIGIFAII
                     SVFTVTDALERKIRSD"
     gene            311..1837
                     /gene="purH"
                     /locus_tag="P5292_07275"
     CDS             311..1837
                     /gene="purH"
                     /locus_tag="P5292_07275"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015782648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRS38967.1"
                     /translation="MSSEKKIGSALISVYHKDGLEPIVRRLHELGVTLYSTGGTQDFI
                     EKMGIPVVPVEQLTNYPSILGGRVKTLHPKVFGGILSRRGHENDVAEVAEYAIPDIDL
                     VIVDLYPFEQTVTSGAGESDIIEKIDIGGISLIRAGAKNFNDVLIVSSREQYTDLLKI
                     LEHSSGRSSLDDRKRFAAQAFLTSSHYDTAIFQYFNRTAAVPAFAQSILESRGLRYGE
                     NPHQQARFFGSFDAFFDQVHGKEISYNNLLDIDAAVALIAEFDEPTFAILKHNNACGV
                     ASRDLLLDAWKAALAGDPVSAFGGILVCNREVTEPVAQAMHDLFFEVCIAPAYNAGAL
                     EILKQKKNRIILIQKQFSLPPRQFRTILNGVAEQDRDVSTETAEQMKPMTRKVPTAAE
                     LRDLVFANKVVKHTKSNTIVLAKDRQLLASGTGQTSRVDALQQAIAKARHFGFSLDGA
                     VMASDAFFPFPDCVQIACEAGIRSVVQPGGSIKDQESVDYCEAHEMAMVMTGIRHFKH
                     "
     gene            1964..>2822
                     /locus_tag="P5292_07280"
     CDS             1964..>2822
                     /locus_tag="P5292_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRS38968.1"
                     /translation="MGFFDFLTQEIAIDLGTANTLIIHNDKVVVDEPSIVAIDRMTGK
                     VIAVGKQAMMMLGKTHENIKTIRPLKDGVIADFDAAEHMIRGMIRMINPARRLFQPSL
                     KMVICIPSGITEVEKRAVRDSAEHAGAKEVYLIHEPMAAAIGIGIDVEEPMGNMIIDI
                     GGGTSEIAVIALGGIVCDQSIRVAGDVFTNDIWDYMRRQHNIMIGERSAEQIKIQVGS
                     ALPELDNPPPDFAVHGRDLMTGIPKEITVSYQEISHALDKSISKIEEAILKALEMTPP
                     ELSADIYRTG"
BASE COUNT          676 a          771 c          765 g          610 t
ORIGIN      
        1 atcggaacgg atcttccgct cgagggcatc cgtgacggtg aaaaccgaga tgatggcaaa
       61 aatgccaatg gtgatgccca gtaacgaaag gagggtacgt agtttattga cgcgcaaagc
      121 ttcgaaggcg aagaggaaac tttccctgaa aaggcgtaag aaaatgcgga agcgcgacat
      181 tgaggcaaat gtaagcagcg caacttcggc cgatggatac ttaacgcaaa ttaacgtcgt
      241 ggatgcagtt gaattccgga aagggcaggg cccgaatttt ctacctttgc cccagccaaa
      301 aacacccttc atgtccagcg aaaaaaagat cggtagcgcc ctgatctcag tctatcacaa
      361 agacggcctc gaaccgatcg ttcgcaggtt gcatgagttg ggtgtaaccc tctacagcac
      421 cggcggaacc caggatttta ttgaaaaaat gggcattccg gtcgttccag tggagcaatt
      481 gaccaattat ccctcgatcc ttggcggccg ggtcaagacc cttcatccga aggtttttgg
      541 cggtatcctg agccgtcgcg gccacgaaaa cgatgtcgcg gaggtagccg aatatgccat
      601 tccggatatc gacctcgtga tcgttgattt gtatccgttc gaacagactg ttacgtccgg
      661 agcaggggag tccgacatca tcgaaaaaat agacatcggt gggatctccc tgatccgggc
      721 tggtgctaag aacttcaacg atgtgctgat cgtttcttcc cgtgaacaat acacggatct
      781 gttgaagatc ctcgaacaca gctccggtcg cagctcgctg gatgaccgga agcgctttgc
      841 ggcacaagct ttcctgacgt cctcgcatta cgatacggcc atctttcagt atttcaaccg
      901 taccgcggca gttcccgctt tcgcgcaaag catcctcgaa tcgcgcggac ttcgctacgg
      961 ggagaatcct catcagcagg cacgcttctt cggcagtttc gatgcgttct tcgaccaggt
     1021 gcatggtaaa gagatctctt acaataacct gctcgatatc gatgcggccg tagccctgat
     1081 cgcggagttt gacgaaccga cgttcgccat tctcaagcat aacaatgcct gcggggttgc
     1141 cagtcgggac ctcctgctgg atgcctggaa agcagcatta gccggcgatc ctgtttccgc
     1201 gttcggcggc attcttgtgt gtaaccggga agtgacggaa cccgtcgccc aggccatgca
     1261 cgacctcttc ttcgaagttt gcatcgcgcc ggcctacaat gccggagcac tggagatcct
     1321 gaagcaaaaa aagaatcgta tcatattgat acagaagcag ttttctcttc cgccccggca
     1381 gttccggacc atcctgaacg gggttgcgga gcaggatcgc gatgtttcga ccgaaacggc
     1441 cgaacaaatg aagccgatga cccgcaaagt gccgacggct gctgaattac gtgacctggt
     1501 gttcgcgaac aaagtggtca aacacaccaa atcgaatacg attgtcttgg ccaaagaccg
     1561 ccagttactg gccagtggca ctggtcagac atcgcgcgta gacgctttgc agcaggccat
     1621 tgccaaggct cgtcacttcg gtttttcact tgatggcgcg gtgatggcca gcgacgcgtt
     1681 cttcccgttc cccgactgtg ttcagatcgc ttgtgaggcc ggcattcgtt cggttgtaca
     1741 gcccggcggt tccattaaag accaggagtc ggtcgactac tgcgaggcgc atgagatggc
     1801 catggtcatg acgggtatcc gccacttcaa gcactaagga ttttaccggg aaattctttc
     1861 cgattcccga atgcaaaaca caacctatta acttcgcacc cgtatatagg cgaccgggag
     1921 tgtgtcctct gtcccctccc caaaaacaac aacaggaccc cacatgggat tttttgattt
     1981 tctcacccag gaaattgcca ttgacctggg taccgccaac acgctgatca tccacaatga
     2041 caaagtggtg gtggatgaac cctccatcgt tgccattgac cggatgaccg gaaaggtgat
     2101 cgcggtcggc aagcaggcca tgatgatgct tgggaagacc catgagaaca tcaagaccat
     2161 tcggccgctg aaggacggcg tgatcgcgga tttcgatgcg gcagagcaca tgatccgtgg
     2221 aatgatccgg atgatcaatc ctgcgcgccg gttattccag ccatccctga agatggtcat
     2281 ttgtattcct tccggtatta ccgaagtgga aaagcgtgcc gtacgcgact ccgccgagca
     2341 tgccggagcc aaagaggttt atctcattca cgagccgatg gcagccgcga tcggcatcgg
     2401 gatcgatgtg gaggagccga tgggcaatat gatcatcgac atcggcggtg gtacaagcga
     2461 aatcgcggtg atcgcccttg gcggcattgt ctgcgaccaa tccatccgtg tggccggtga
     2521 cgtcttcacc aacgacattt gggattacat gcgccgccag cacaatatca tgatcgggga
     2581 acgatcggcg gaacagatca agatccaggt tggatcggct ttgcccgaac tcgacaatcc
     2641 tccgcccgat ttcgcggtac acggtcggga cctcatgacg ggtatcccga aggagatcac
     2701 cgtcagctac caggagatct cacatgcgct cgacaaatcc atctccaaga tcgaggaggc
     2761 catcctgaag gcgttggaga tgacgccccc cgaactctcc gctgatattt accggacggg
     2821 ca
//
LOCUS       DAOTON010000333         2822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27814_length_2822_cov_3.497289, whole genome shotgun sequence.
ACCESSION   DAOTON010000333 DAOTON010000000
VERSION     DAOTON010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27814_length_2822_cov_3.497289"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..530
                     /locus_tag="P5292_07285"
     CDS             132..530
                     /locus_tag="P5292_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRS38969.1"
                     /translation="MAYWLVKSDPDTYGWSDLVRDKKTTWDGVRNHLAKRHLSGMKKG
                     DQVLFYHSGKESAIVAIAEVANAPKPEPNAPDWICVDLVPLKPLKQPVTLKAIKAEKK
                     LADFPLVKIGRLSVMPVAEAAFDLLVAKGS"
     gene            complement(536..862)
                     /locus_tag="P5292_07290"
     CDS             complement(536..862)
                     /locus_tag="P5292_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38970.1"
                     /translation="MRYFRLVIPMLPVTAMALFPFVLFDRHDRASDQILLRHEQIHLR
                     QQIELLILPFYLLYAYYYLKGRFKGHSAREAYRAIPFEREAYLHEATQDYLNSRSHFA
                     WMKYRK"
     gene            987..>2822
                     /locus_tag="P5292_07295"
     CDS             987..>2822
                     /locus_tag="P5292_07295"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS38971.1"
                     /translation="MRHLLLLLILAFFTTGALAQTGDIRGFVYEQETSEPAIYTSVYL
                     KGTTHGVQTNLDGYYSITRVPPGDYVLMVTSVGFDTIKEPITIKAGDILTKKLYLRKS
                     AIQMKEIEISGENEEKKTEVKVSVNKVTPKEIKQLPTVGGEPDLAQYLQVLPGVIFSG
                     DQGGQLYIRGGTPIQNKVLLDGMIIYNPFHSIGLYSVFDADIIRGADVYTGGFNAEYG
                     DRISSVMDITTRDGNKKRIAGRASVNPFTAKFLLEGPLKKEKENQEGSSSFIITSRNS
                     YLDKTSKSLYSYVDTAGLPFAFNDLYGKISLNSSSGSKVNFFGFNFTDRVNYKEIADL
                     DWKSFGLGTNFVLVPSGSSVLIDGNFAYSNYRINLDERDEQSRFSEISGFNTSLNFSY
                     FIKKDELRYGLEIVGFRTDFQFSNAYSKSIGQTENTTQLAGYFKYKKIAGKLIAEPGI
                     RINYYSSLSELTAEPRLGLKFNATDKLRFKAAGGYYSQNLLSGTSDRDVVNLFYGFLS
                     GSDNIPETFDGEDVTSRLQKAQHAIAGFEIDLPFHLNLNVEGYFKNFSQLQNLNRDKV
                     YDDDSENSEKPDYLKKDFIIESGTARGIDFLLKYDYRGLYLWATYS"
BASE COUNT          720 a          794 c          665 g          643 t
ORIGIN      
        1 gggagattag taattagtaa ttagtaatta gtaattagtg atgggtgatt gggggttcgt
       61 aatttgtttg gctgaatttc cctcatccct catccctcat ccctcgtccc tcgtccctcg
      121 tccctcgtcc catggcttac tggcttgtaa aatccgaccc cgacacctac ggctggtctg
      181 atcttgttcg tgataagaaa accacctggg acggtgttcg caatcatctt gcgaaacgtc
      241 atttatccgg aatgaagaaa ggcgatcagg tgcttttcta tcacagcggc aaggaatcgg
      301 ctattgtagc cattgccgag gtcgccaatg ctcccaaacc ggagccgaat gctcccgact
      361 ggatctgtgt tgaccttgta ccgcttaaac cattgaaaca accggtgacc ctcaaagcga
      421 tcaaagccga gaaaaaactc gcagactttc cgctggtgaa gatcggtcgc ctttccgtga
      481 tgcctgttgc ggaagcagcg tttgatttgc tggttgccaa gggctcctga cccgactatt
      541 tcctgtattt catccaggcg aagtgggaac ggctgtttag atagtcttgc gtagcctcat
      601 gcaggtaagc ttcgcgctcg aaagggatgg cccgataagc ctcccttgcc gaatgccctt
      661 tgaatcgccc tttcaaatag taataagcgt acaacaggta aaacggcaat atcaacaatt
      721 caatctgctg ccggaggtgt atctgctcat gccgcagcag gatctggtcg gaagcccggt
      781 catgccgatc gaatagtaca aacggaaata aagccatcgc ggtaacagga agcatcggaa
      841 tgaccaaccg gaagtagcgc atgacattgc caggacaatt acccggccaa agtgttaaag
      901 tatgtttctt tcatcgtccg aatgtcaaag cgacccatta cgactctgac atccgggaaa
      961 caatactatt tttggcgctc tcaattatgc gtcacttact tcttctccta atcctggcgt
     1021 tcttcaccac aggcgcgctc gctcagaccg gcgacatccg gggctttgtc tatgaacagg
     1081 aaacatccga acccgccatc tataccagtg tctatctaaa aggcaccact cacggtgtcc
     1141 agaccaacct ggacggctac tattccatca cccgcgtccc cccgggcgat tatgtgttga
     1201 tggtcacctc ggtcggcttc gataccatca aggagccgat caccatcaag gccggcgata
     1261 tcctcacgaa aaaactatac ctccgcaagt cggcgatcca gatgaaagag atcgagattt
     1321 ccggcgagaa cgaggaaaag aagaccgagg taaaagtttc cgtcaacaag gttacgccca
     1381 aggaaatcaa gcagcttccg accgtaggcg gcgaacccga tcttgcccag tatctgcagg
     1441 tactgcccgg tgtgatcttc tccggcgacc agggcggaca attgtacata cgcggcggca
     1501 ccccgatcca gaacaaagtc ctgctcgacg ggatgatcat ctataatccg ttccactcca
     1561 tcggcctcta ctcggtattc gacgccgaca tcattcgcgg agccgacgtt tataccggag
     1621 gcttcaacgc ggaatacggc gaccgcatct cctcggtgat ggacatcacc acccgcgacg
     1681 gcaacaagaa aagaatcgcc ggccgcgctt cggtgaatcc gtttacggcc aagttcctgc
     1741 tcgaaggtcc gctcaaaaag gaaaaagaga accaggaagg cagttcgtcg ttcatcatca
     1801 ccagccggaa ttcgtatctc gacaaaactt cgaaaagcct ttacagctac gtggatacgg
     1861 cagggctgcc cttcgccttc aacgacctgt acggcaagat ctcgctcaac tcctcttccg
     1921 gcagtaaagt caatttcttc ggtttcaatt tcaccgatcg ggtgaactac aaggaaattg
     1981 ccgacctgga ctggaaatca ttcgggctgg gaaccaattt cgtgttggtt ccatccggca
     2041 gttcggtcct gatcgacggt aatttcgctt acagcaatta ccgcatcaac ctggatgaac
     2101 gcgatgagca atcacgcttt tctgagatca gcggcttcaa caccagcctg aatttttcgt
     2161 acttcatcaa gaaggatgaa ttgcgctatg gtctcgagat cgtcggtttc cggactgact
     2221 tccagttctc aaacgcctat tcgaaatcca tcggtcaaac ggagaacacc acacaactgg
     2281 cgggatattt caagtacaag aagatcgccg gcaaactgat cgccgaaccg ggcattcgga
     2341 tcaactacta ctcctcgctg tccgaactga cggcagaacc tcgtctgggt cttaaattca
     2401 atgcaacgga caaacttcgt ttcaaagccg ccggcggtta ttattctcag aacctgctca
     2461 gcggcacctc ggaccgggac gtcgtcaacc tgttttacgg ctttctatcc ggcagcgaca
     2521 acattcctga aaccttcgat ggcgaagacg tcacctcacg ccttcaaaag gcgcaacacg
     2581 ccatcgccgg cttcgagatc gacctcccct tccacctcaa cctgaacgtt gaaggatact
     2641 tcaagaactt ttcccaattg caaaacctga accgcgacaa agtgtacgat gatgacagcg
     2701 aaaacagcga gaagcccgat tatctgaaga aggatttcat catcgaaagc ggaacggcgc
     2761 gcgggatcga cttcctgctc aaatacgatt accgcggatt atacctctgg gccacttact
     2821 cg
//
LOCUS       DAOTON010000334         2815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27907_length_2815_cov_4.081159, whole genome shotgun sequence.
ACCESSION   DAOTON010000334 DAOTON010000000
VERSION     DAOTON010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27907_length_2815_cov_4.081159"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2409)
                     /gene="secDF"
                     /locus_tag="P5292_07300"
     CDS             complement(<1..2409)
                     /gene="secDF"
                     /locus_tag="P5292_07300"
                     /inference="COORDINATES: protein motif:HMM:NF009585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRS38972.1"
                     /translation="MQSKGAVKLFAILLALVCVYQLSFTLVTRSVEKDAKAFANGDPA
                     KERQYLDSVNTQPVYPIFGHTYKKCKENELNLGLDLKGGMNVTLEVSLVDLIRSMANH
                     STDATFNKALDNAVAAQSKSQKDFVSLFVDEFQKLDPNGRLAAIFATKELSERINFNS
                     SNEEVKQIIQTEANAAFDRTYQIIKTRIDKFGVTQPNVQKLGNGRILVELPGIKEPER
                     VRKLLQGTAKLEFWETYDNTEIYPMLEEANKALKLTGAADTTVQAEAINTDSLAVPAD
                     TLKDATAAADTAAKSEDLVAQLGDKKEGGDTAKTDTTGQKSFEDFARENPFFAVLTPS
                     VGQNEQGQSELRKGPVIGMSLIKDTARVNQYLAMPQVRSKFPREFKAFWSVKPIDAEG
                     RVLQLVAIKMPNRDFQAPLDGSAVVDARKQKGQFSDNWEIGMSMNAEGARTWKRLTGQ
                     NIGKSVAIVLDDYVYSFPVVQNEISGGNSSITGQFTLEEADDLANVLKVGKLPAPARI
                     VEDTVVGPTLGQEAINAGLISFVIALVLVLLFMVSYYSNAGLVADIALFANVFFIMGI
                     LASLGAVLTLPGIAGIVLTIGLSVDANILIFERVREEMALGKGLRLAISDGFKHAYSS
                     IIDSNVTTLILGIILYVFGTGPIQGFATTLIIGILTSLFCAIFITRMIFEWMLGRNKD
                     IRFWNNFSKGAFKNIHINFVERRKIYYMISGLIIAAGIAAFVTKGLNFGVDFQGGRSY
                     VVRFEKPMATVDVIDALKGPFGKSPEVKTFGGENQVKITTTYLIDDPAQDAEVRVTDK
                     LNEGL"
     gene            complement(2555..2746)
                     /locus_tag="P5292_07305"
     CDS             complement(2555..2746)
                     /locus_tag="P5292_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38973.1"
                     /translation="MVVHSNTLSAAPRHDLPFKGALRFAGVFLFYALLFLLISLITHP
                     EVLHPQDAGSGGVQQEQGK"
BASE COUNT          612 a          762 c          802 g          639 t
ORIGIN      
        1 cagaccctca ttcagcttgt ccgtcacacg cacttcggca tcctgtgcgg gatcgtcgat
       61 caggtaggtg gtggtgatct tcacctggtt ctcgccgccg aaggtcttta cttccggaga
      121 tttgccgaac gggcctttga gcgcgtcgat cacgtcgacg gtcgccatcg gcttctcgaa
      181 gcgcaccaca tagctgcggc cgccctggaa gtcaacaccg aagttcagac ccttggtcac
      241 gaacgcggca atacccgccg cgatgatgag gccgctgatc atgtagtaga tcttccggcg
      301 ctccacgaag ttgatgtgga tgttcttgaa ggcgcctttg gagaagttgt tccagaagcg
      361 gatgtccttg ttacgaccga gcatccattc gaagatcatg cgcgtgatga agatagcgca
      421 gaacagcgaa gtgagaatac cgatgatcag cgtcgtcgcg aatccctgga tcggacccgt
      481 accgaataca tacaggatga taccgaggat cagggtcgtg acgttggagt cgatgatcga
      541 agagtaagcg tgcttgaatc cgtcggagat cgcgaggcga agtcctttgc ccagcgccat
      601 ctcttcccgc acacgttcga agatgaggat gttcgcgtcc accgagagac cgatcgtgag
      661 gacgataccg gcgatacccg gcagcgtcag cacggcgccc agcgaggcga gaatgcccat
      721 gatgaagaac acgttcgcga agagggcgat gtcggcaacc agaccggcgt tgctgtaata
      781 cgaaaccatg aacaacagca ccagcaccag cgcgatcacg aacgaaatga gaccggcgtt
      841 gatggcttcc tgaccaagcg tcggacccac gacagtatct tccacaatgc gtgcgggagc
      901 cggcagttta ccgactttca gcacgttcgc gagatcgtcc gcttcttcca gcgtgaactg
      961 accggtgatg ctggaatttc cacccgagat ctcgttctgt acgaccggga aggaatagac
     1021 gtaatcgtcg agtacgatgg ctaccgattt gccgatgttc tgacccgtca accgtttcca
     1081 ggtgcgtgcg ccttccgcgt tcatactcat gccaatctcc caattgtcgg agaactggcc
     1141 tttctgctta cgcgcgtcga cgaccgcgga accgtcaagc ggagcctgga agtcgcggtt
     1201 cggcatcttg atggcaacga gctgcagcac gcggccttcc gcgtcgatcg gcttcaccga
     1261 ccagaacgcc ttgaactcac gcgggaattt cgaacgcacc tgcggcatgg cgaggtactg
     1321 gttcacacgg gcggtgtcct tgatcaacga catgccgatg accggccctt tgcgcagttc
     1381 cgattgtccc tgctcgttct gtccgaccga aggggtcagc acagcgaaga agggattctc
     1441 acgcgcgaag tcttcgaacg acttctgtcc ggtggtatcc gttttcgcgg tatctccgcc
     1501 ttctttcttg tcgccgagtt gggcaaccag gtcttccgac ttggcagccg tatccgctgc
     1561 ggcagtcgcg tctttcaacg tatcagccgg aacagcgagg ctgtcggtat tgatcgcttc
     1621 agcctgcacc gtggtgtcgg cagcacccgt cagcttgagg gccttgttgg cctcttccag
     1681 catcgggtag atctcggtgt tgtcataggt ctcccagaac tcgagcttcg ccgttccctg
     1741 gaggagcttg cgaacacgct cgggttcttt gatacccgga agctcgacga ggattcgtcc
     1801 gttgcccagc ttctgtacgt tcggctgggt aacgccgaac ttgtcgatac gggtcttgat
     1861 gatctggtag gtacgatcaa aggcggcatt cgcttccgtc tgaatgatct gcttcacttc
     1921 ctcattcgaa gagttgaagt tgatgcgttc ggacaattcc ttggtcgcga aaatggccgc
     1981 gagacgaccg ttcggatcga gcttttggaa ttcatcgacg aacagggata cgaagtcctt
     2041 ctgactcttc gactgggcgg caacggcgtt gtccagggcc ttgttgaagg tggcgtccgt
     2101 actgtggttg gccatcgaac ggatcagatc caccagcgac acttcaaggg tgacgttcat
     2161 gccgcctttg aggtcaagac cgaggttcag ttcgttctct ttacacttct tgtacgtgtg
     2221 accgaagatc ggatacaccg gctgggtgtt caccgagtcg aggtactgac gctccttcgc
     2281 cggatctccg ttggcgaacg ccttggcatc cttttcaaca ctgcgcgtga ccagcgtaaa
     2341 tgatagctgg taaacacaga ccagggccag caggatcgca aacagtttta cagctccttt
     2401 ggattgcatg gatgataatt gggttaatag aatatgagtg gcttacgccc aatttttttg
     2461 agcgtgcaaa gatagaattt acaggcttaa atccaatttt aaacggaaat cggcctttta
     2521 tcggcagaag tggaattccg ggcaaaaaag gcgattactt tccctgttcc tgctgcacgc
     2581 cacccgaacc cgcgtcttgc ggatgcagga cttccggatg cgtgatcagg ctgatcagca
     2641 ggaataacag ggcgtaaaag agaaagacgc cggcaaagcg caaggctcct ttgaacggca
     2701 ggtcatgccg gggagctgcg gaaagggtat tgctgtgaac taccataaag tgtctctaaa
     2761 tcaatcagat gtaaataaaa tcagggttga agggatccaa gcagggaggt ttccg
//
LOCUS       DAOTON010000335         2815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27909_length_2815_cov_3.531159, whole genome shotgun sequence.
ACCESSION   DAOTON010000335 DAOTON010000000
VERSION     DAOTON010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27909_length_2815_cov_3.531159"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="P5292_07310"
     CDS             <1..200
                     /locus_tag="P5292_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38974.1"
                     /translation="TVLTVTNTLSDRLEENVLHDFEGHILVTIARYLKVKAVNYGRLP
                     IWHPGAGYPAFIFVDEVWANP"
     gene            725..883
                     /locus_tag="P5292_07315"
     CDS             725..883
                     /locus_tag="P5292_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005420081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HRS38975.1"
                     /translation="MPFWLLIVLCILLPPLAVFLKFGIGTEFWISLILTLIFWLPGVI
                     YALIVVTR"
     gene            complement(953..1675)
                     /locus_tag="P5292_07320"
     CDS             complement(953..1675)
                     /locus_tag="P5292_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38976.1"
                     /translation="MIFSPVNLQDELIRERMRQQRIMNDVHDLFDAGTRRDQEVLDRL
                     AGRGRPTEPQLALDAADLAHVFTLDQIRQTCIRFRLRFLDTRFFRSEFPYDAVLKIRA
                     FEERYNIRIERFRIAAPDHAFKLENINKDPLLFAELADGRYYLLHQWGEDLAWYKRFL
                     LWPLQNLKTFLLTILSISFLISFLIPSAVMHVFTLQSEIYLRIWFGLHLFIGFTGIGL
                     WAGISFDKSFSSMSWNSQYRNY"
     gene            1828..>2815
                     /locus_tag="P5292_07325"
     CDS             1828..>2815
                     /locus_tag="P5292_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HRS38977.1"
                     /translation="MRLILSFLLLLSALALFAQPCKEVVGYYPAWQWYDRAKLVKPAG
                     IRYDRYTIINYAFFQPMPDGSITGTDAWADENLLLGEPDWANGGYLPNTSIVDLAHSA
                     GVKVVISVGGWTLSNDFPAIAADPVKRTNFAHHCAQLIRQYNLDGVDLDWEYPGFPDH
                     NGTPADRANFTLLLSEVRDSIDATGNTLGRNMLLTAAVGAGADRMDDVDWPAVTSLLD
                     IINLMSYDFFGAWDATTNHNAPLYAPNVGDVTFNCDSAITRLIQVYGVDPGRITLGVP
                     FYGRSAVTTGAPGLFVPSTQTADNITFFTDDGSPLYYNILLNLSSFDTWWDNQ"
BASE COUNT          622 a          747 c          747 g          699 t
ORIGIN      
        1 agaccgtatt gaccgtaaca aatacgcttt ccgatcgact ggaggagaat gtgctgcatg
       61 atttcgaagg acatattctc gtcactattg cccgttactt gaaagtgaag gcggtgaatt
      121 atggacgatt gcctatctgg catccgggcg caggttatcc tgcgttcatc ttcgttgatg
      181 aagtgtgggc gaatccctga cgcaaccaac agtttcccgg ttcataactc cggcaacgcg
      241 agattgatac agcccgaagc tgttttactt ttgttcaaaa tcacgcacga tgaaacgtat
      301 tctattcctt gcagtggccg ttgtttcgct gcttaccgcc tgcagtcctg atctgcacct
      361 cgacatttca aaacgccgct atcgtaaggg ctttcacgtt gatgccggca gcggacaccg
      421 tcaaagcgtt acggctgaac gtcaggttga acaaacggca aagatgccgg tgatcgtctc
      481 tgccttaccg caagcgcaaa atgagccgct cgtcgagcaa cactattctg ttcctgttgt
      541 tgaagcacag cctccggtag tcgcctcgaa cgaaaccgtg acgcaggtga agcatacccg
      601 ttcaaccccg gcagcggtgg atgcaacctc aacgttgttt gcgaaagaac agtcggtaca
      661 atccgcttcc aaatttcagc aagtgaagca ggcggctaaa cgctttttcc atccgcagga
      721 agatgtgccg ttctggctgc tcatcgtatt gtgcattctc cttcctccgc ttgcggtttt
      781 cctcaagttc ggaatcggaa cagagttttg gatcagtctt atcctaaccc tgatcttctg
      841 gctgcccggc gtgatctacg cgctgattgt cgttacgcgt tgatgacccc tggtaccata
      901 cgaaagcggc cctgctatac agatagcagg gccgcttttg ttattgtgaa aatcaatagt
      961 tgcggtattg actgttccag ctcatgctgg agaaagattt gtcgaacgag atgcctgccc
     1021 agagaccaat tccagtgaag ccgatgaata ggtggagtcc aaaccagatg cgcaggtaga
     1081 tctcgctttg gagggtaaac acgtgcatca ccgcggaggg aatcagaaat gatatcagga
     1141 agctgatgga aaggatcgtc agcaggaagg tcttgaggtt ttgtaatggc cagagcagga
     1201 atcgtttgta ccaggccagg tcttctcccc actgatgcag aagatagtat cgaccgtccg
     1261 ccagttcggc aaagagcaag gggtctttgt tgatgttctc cagtttaaaa gcatgatccg
     1321 gcgccgcgat gcggaagcgt tcgatccgga tgttgtatcg ttcttcgaag gcgcggatct
     1381 ttaatacggc atcgtatgga aactcggacc ggaagaaacg cgtgtccaga aaccgcagcc
     1441 ggaagcgaat gcaggtctgc cggatctggt caagtgtgaa tacatgcgcc agatccgccg
     1501 cgtcaagcgc cagctgcggt tccgtcggcc gtcctcgacc ggccaagcga tccagtactt
     1561 cctgatcacg gcgtgttcct gcgtcgaaca gatcatgtac atcattcatg atccgctgct
     1621 ggcgcatccg ttcacggatc agttcatctt gtaagttgac gggggaaaag atcatacgca
     1681 gcttataaca cttaaagata gcaggtggtt caggctgaaa ggctattact attccgtgaa
     1741 ataactgcgt atttttaagg acttctatgg atgagccttc atgtttgaca tggaatcgct
     1801 cgctttcttc ggtaataaaa tctgatcatg cggttgattc tcagtttcct gctcctgctg
     1861 tcagcgctgg cgctgtttgc gcagccttgt aaagaggtcg ttgggtatta ccctgcctgg
     1921 cagtggtacg atcgggctaa actggtgaag cctgccggta tccgatacga ccgttatacg
     1981 atcatcaact acgcgttctt tcagcctatg ccggatggtt ccattaccgg cacagatgcc
     2041 tgggccgatg agaacttgct gctgggtgag cccgactggg cgaatggtgg ttacctgccg
     2101 aatacctcga tcgtcgatct ggcacatagc gcgggggtga aagtcgtgat ctctgtcggc
     2161 ggctggacgc tttcgaatga ctttcctgcc atcgctgcgg atcccgtcaa acggacgaac
     2221 ttcgcccacc actgtgcaca gctgatccgg cagtataacc ttgatggtgt cgatctcgac
     2281 tgggaatacc ccggtttccc ggatcacaac ggcactccgg ccgatcgtgc caattttacc
     2341 ttgctgcttt cggaggtccg tgactcgatc gatgcaaccg gaaacaccct cggccggaac
     2401 atgttgctga ccgctgccgt cggcgctggg gctgaccgga tggacgatgt cgactggccc
     2461 gcggtcacgt ccttactcga tatcatcaac ctgatgtcgt acgatttctt cggtgcctgg
     2521 gatgctacca cgaatcacaa tgcacccctc tatgcgccca acgtaggcga tgtcacgttc
     2581 aactgcgaca gtgccatcac ccgcctgatc caggtgtatg gagtcgatcc cggaaggatc
     2641 acgctgggtg ttccatttta cggacgttcc gccgtcacca cgggagcgcc gggattgttc
     2701 gtcccttcca cgcaaacggc tgataacatc acgttcttta ccgatgacgg gtcgccgctg
     2761 tattacaaca ttctcctgaa cctttcttcc ttcgatacgt ggtgggacaa tcagg
//
LOCUS       DAOTON010000336         2810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_27967_length_2810_cov_3.733938, whole genome shotgun sequence.
ACCESSION   DAOTON010000336 DAOTON010000000
VERSION     DAOTON010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27967_length_2810_cov_3.733938"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="P5292_07330"
     CDS             <1..181
                     /locus_tag="P5292_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRS38978.1"
                     /translation="VDEIGKYLGVSSDTVYRSIDKHGMPAHRVGRLWKFKKEQVDAWV
                     EAGGAAADKNKNNED"
     gene            201..959
                     /locus_tag="P5292_07335"
     CDS             201..959
                     /locus_tag="P5292_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38979.1"
                     /translation="MDKQFSTIGYGFLLEETLMLLDAALESNDAQAVRGSCIQRWPQK
                     SESNKEKLWQHMKYRFLEIENNSIKNTSFLKMFRKIRSNDEAVQDLVFFQLCITTPIL
                     FETLSLLVTDSFLNTGEAVFSRYHLDQLLERKYERMPQSTRDRVRSILVKAGRLKLSK
                     SNYSVTTYCPTEAVLGYALYHDASKNGWRAPSTATVINEGTIAPIFMCNRALLIAGVN
                     KLATKGHCEYHRHGSTDQVQLIHQSLEEYVDAWR"
     gene            940..1485
                     /locus_tag="P5292_07340"
     CDS             940..1485
                     /locus_tag="P5292_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38980.1"
                     /translation="MSMPGDNKTTPAQAKALNELIGLVGESHVGGRTLCCLIGGQGSG
                     KTWLAEHMASLGDFGQVRYVSVNDIVLDLLAKDPMFAEVFDFNATTVPADLKRYGNRL
                     RKAVHDLVAEQLLPEGLTILDHLELVFALGMDPMTTWYNDAVGKRRILLVLTGRMAGQ
                     LCRCGQYTLTRGDQPIVELEA"
     gene            1601..>2810
                     /locus_tag="P5292_07345"
     CDS             1601..>2810
                     /locus_tag="P5292_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38981.1"
                     /translation="MLPEVEESARIILDRIHRKQGRGFFITGLYGSGKTIFMAWLSAI
                     LTEPTLRKQFLDTKPEWGMDALAPRKFLTINFTAIEVPDETLEQAFWNSVTDHLRHLS
                     PPVTATLSNTDAYLDTFFNHTASGTRSDVENWIRDNRSLSVDQLRAYDKEYQKTIIEQ
                     AVAALNIQLVQDRTTVTEKVKKLVEIAKERGYEGVLVFIDELYLHLIQSDEHFNRGTA
                     FLGQLAEAGLAGDQPFWIFGAVQEEIQAIARQAGRNYDTELMGKLSGQSGRFQSINIP
                     VTQFHRIYNHRLFRENSKCVHKLADAFKAEIQPHFRGAFTDFLKRYFREREPITDEAK
                     HFADVYPMHPFGLYCLTAITNRGGRSRGALGFVQEFIERRLQDTSDWKRIAVLDDVFD
                     YEDLRNKIIQD"
BASE COUNT          701 a          776 c          725 g          608 t
ORIGIN      
        1 agtagacgag ataggcaagt acctcggtgt cagtagtgac accgtgtacc gctcgataga
       61 caagcacggc atgcccgctc atcgagtagg ccgactctgg aagtttaaaa aagaacaggt
      121 cgatgcttgg gtcgaggctg gcggtgctgc agcagacaaa aacaaaaaca atgaggacta
      181 gatgataggc ggcaatttca atggataagc agttttcaac aatcggatac ggattccttc
      241 tggaagagac tctcatgctg ctcgacgccg cattggagag caatgacgcg caagctgtgc
      301 gtggctcctg tatccaacgt tggcctcaga agtccgagtc caacaaggaa aagctgtggc
      361 aacacatgaa atatcgcttc ctcgaaatcg agaacaactc gatcaaaaac acttcattcc
      421 tgaagatgtt tcgaaagatt cgcagcaatg atgaagcggt tcaggatctg gtgtttttcc
      481 agctatgcat tactactccg attctttttg aaaccctttc acttttggtt acagacagct
      541 tcctgaatac cggcgaggcc gttttctctc gctaccacct cgaccagctc ctggaacgca
      601 aatacgagcg catgcctcag tcaactcgag atcgagtgcg gtcgatcctc gttaaggcag
      661 gacgcctgaa gctttcgaaa tccaattact cggtgaccac ctactgccca accgaggccg
      721 ttttgggcta cgcgctctat cacgacgcat caaaaaacgg ttggcgggca ccatcgacag
      781 ccacagtcat caacgaaggg accatagctc ccatcttcat gtgcaaccgc gccttgctga
      841 ttgccggggt caacaaactc gcaaccaagg ggcactgtga atatcaccgg cacggaagca
      901 cggatcaagt tcaactgatt catcaatcgc tggaggagta tgtcgatgcc tggagataac
      961 aaaacgactc cagctcaggc caaggcgctc aatgagctta taggattggt cggcgaaagc
     1021 cacgttggcg gacggactct ttgctgcctg attggcggtc aaggttccgg aaaaacatgg
     1081 cttgcggaac acatggcatc gctgggcgat tttgggcagg tccgatatgt gagcgtgaat
     1141 gacatcgttc tcgacctact ggctaaagat cccatgtttg ccgaggtgtt cgatttcaat
     1201 gctacgaccg tcccggcgga tcttaaacgc tacggaaaca gacttcggaa ggcggtacac
     1261 gaccttgtag ccgaacagct attaccagag ggactgacca tcctcgatca cctggaactg
     1321 gttttcgcgc tcggcatgga ccccatgact acctggtaca acgatgccgt gggaaaaaga
     1381 agaattttac tggtgctgac aggacgaatg gctggtcagc tttgccgctg cgggcagtac
     1441 accctgactc gcggagatca accaattgtg gagctggagg cctgatatgc cactcatgaa
     1501 ggatatgttt gatttcacgc cagtcagtag ctgggtggaa aacttcaacg agtcgactca
     1561 cgattcagcc agccggacgc ggattttcga aagcttcgtc atgctgcccg aggttgagga
     1621 gtcggcccga attatcctgg accgaattca ccgtaagcaa ggtcgcggct tcttcatcac
     1681 cggtctatac ggttccggta agaccatctt catggcatgg ctatccgcca ttctgaccga
     1741 accgacgctg cgcaaacagt tcctcgatac caagcccgaa tggggcatgg atgcgctcgc
     1801 accacgaaaa ttcctcacga tcaactttac cgccattgaa gtgcctgacg agaccctcga
     1861 gcaggccttc tggaactcgg ttactgacca cttgcgccac ctctcgccgc cagtaacggc
     1921 gactttgtcc aacaccgatg cctatctgga tacctttttc aatcacaccg ccagcggcac
     1981 ccgcagcgat gtggagaact ggattcgtga caaccggagt ctctccgtcg atcagcttcg
     2041 cgcctatgac aaggaatatc agaagaccat cattgagcag gctgttgccg ccctgaacat
     2101 tcaactcgtc caggaccgca ccacggtcac ggaaaaggtc aagaagctgg tcgagatcgc
     2161 caaagaacgt ggctacgaag gcgtcctggt ctttattgac gagttgtatc ttcacctgat
     2221 ccagagcgac gaacacttca accggggcac tgctttcctt ggacaactgg ccgaagctgg
     2281 actggccggg gatcaacctt tctggatttt tggcgcggtc caggaggaaa ttcaggccat
     2341 cgctcgccaa gccgggcgca attacgatac agagctgatg ggcaagcttt ccgggcaatc
     2401 cggccgcttc cagtccatca acatcccggt cacgcaattt catcgcatct acaatcaccg
     2461 gctgtttcgt gaaaattcca aatgcgttca caagctcgcc gatgccttca aggctgaaat
     2521 tcagccgcat ttccggggag cctttactga cttcctgaaa cggtatttcc gcgaacgcga
     2581 acccatcacc gacgaggcaa aacactttgc cgacgtttat ccgatgcatc ccttcgggct
     2641 gtactgcctc accgcgatca ccaaccgggg cggaagatcc cgtggcgcac tgggatttgt
     2701 ccaggaattc attgaaagac gtctgcagga taccagcgac tggaaacgaa tcgcggtgct
     2761 cgacgacgtg ttcgactacg aggatctgcg taacaagatc atccaggacg
//
LOCUS       DAOTON010000337         2801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28066_length_2801_cov_3.791697, whole genome shotgun sequence.
ACCESSION   DAOTON010000337 DAOTON010000000
VERSION     DAOTON010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28066_length_2801_cov_3.791697"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1767
                     /locus_tag="P5292_07350"
     CDS             <1..1767
                     /locus_tag="P5292_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS38982.1"
                     /translation="ISLLLYVINLQLLFTLLQRLFRQVHTLFLALIVALFAAHPIHSE
                     VVANIKSQDELLAAAFGLLAWLKFLPKQENVSIGRSDVITGSLYFFLALLSKESAIAF
                     ALIIPASRILVLKQPPGSTLLHAVPLLVLGLIFLWIRSLYVPFSIDASADPLLANVLN
                     GAKNMGETSATKMVILLQFLRAVFLPWPMSWDYSFNEIPISTWSQLTPWLGLLLYALL
                     MVVVLFRFRQWPIVSFCILFFFISTSPTNNLFFTTGSTFAERFLYVPSIAFCIVLVFL
                     IAKALKTDIRQFPEHGRRPLFLVVGTLVLAGSVLTVVRASDWRNNKTLFSADILHAPN
                     STRTHYSLASELMKEAQRSVDYRKRTELLNESAASFDRSIAIHPGNFQAWYNYGLNRA
                     LAGDTVKAVNCYRRSIELSPTYQVAMNNLAVIYDAQGATDSAAAWYRRVLALDPDNAV
                     AKSNLSNTYFNQGLRLSQVGRRDEAITAYRKSAASDPSNSIPLNNLASLFASEAQYDS
                     ALVYLKKAYSIKPGEMMVIENIAAVSYLDGNYPQAMEFAGKALAIQPNAQRSLGVMAD
                     TYQAMGNPAEAMKYLQRLQQIR"
     gene            1910..2722
                     /locus_tag="P5292_07355"
     CDS             1910..2722
                     /locus_tag="P5292_07355"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRS38983.1"
                     /translation="MKLHHSFCLAIACCVATASSALNPSKEYKVKPEKYGMNYKEEKV
                     PTKDGASLNAWFFETAKKTTNWMVISGSGDGNMADDIELAGQFLSAGWNVALYDYRGY
                     GASSDFAIDPDTYIYPQFINDLNAMLDYLRKSRAITKFDLFGKNIGAGLSLGVGSNRT
                     ETRKIIADGPWTGLEPMKKKIKDKKGKEVIIPFGYDKTYEPMYACDKSRPNIKGVLVI
                     VTPQDDLIKPEDTKTLRCATQTYIVANSPSNAENFSTDKNVYFEKVSKFLNQ"
BASE COUNT          672 a          782 c          693 g          654 t
ORIGIN      
        1 atcagtctgt tattgtatgt gatcaacctg caactgcttt tcaccctctt gcagcggttg
       61 ttccgtcagg ttcacaccct gtttctggcc ctgatcgttg ccctgttcgc cgcccatccg
      121 atccatagcg aggtagtggc caacattaaa agccaggacg agttactggc tgccgcattc
      181 ggcctcctgg cctggctgaa attcctgccg aagcaggaga atgtcagcat cgggagaagt
      241 gatgtgatca ccggaagcct gtacttcttc ctggcactgc tatcgaagga aagcgcgatc
      301 gccttcgcac tcatcattcc ggcctcccgc atcctggttt taaaacaacc acctggttcc
      361 accctgctgc atgccgttcc gttactggtt ctcggattaa tctttttgtg gatacgaagc
      421 ctgtacgtac ccttttcgat tgatgcatcc gcggatccgc tattggccaa tgtcctcaac
      481 ggcgcaaaga acatggggga aacaagcgcc actaaaatgg tcatcctcct gcagttcctg
      541 cgggccgtat tccttccctg gccgatgtcc tgggattatt cgttcaacga aatcccgatc
      601 agcacctgga gtcagttaac gccctggctg ggtttattgc tgtacgcgtt gctgatggtc
      661 gttgtccttt tccgctttcg ccaatggccg attgtcagtt tctgcatcct gttctttttc
      721 atctcgactt cgccaaccaa caacctgttc ttcaccaccg gctcgacctt cgcagaacga
      781 ttcctgtatg taccgtcgat cgccttttgc atcgtcttgg tattcctgat cgcaaaagcg
      841 ctgaagaccg acatccgcca atttcccgaa catggcagac ggccgctctt tctggtcgtc
      901 ggcacgctgg tgctggcagg gtcggtgctc accgtggtgc gtgcttccga ctggaggaat
      961 aacaagacgc tgttcagcgc cgacattctc catgcaccga acagcacccg gacacactac
     1021 agcctcgcct cggaattgat gaaggaagca caacgctccg tcgattaccg gaaacgcacc
     1081 gaactattga acgaatcggc tgcctcgttc gatcgcagca ttgcgatcca tccgggaaat
     1141 ttccaggcct ggtacaacta cggtctgaat cgcgcgcttg caggcgatac tgtcaaggcc
     1201 gtcaattgtt accggcgatc gatcgaactc agtcccacct atcaggtggc catgaacaac
     1261 ctcgctgtga tctacgatgc gcaaggggcg acagattccg ccgcagcctg gtatagacgg
     1321 gtccttgcgt tggatcccga caatgcggtc gccaaatcga acctctcgaa cacctatttc
     1381 aaccagggat tacggctcag ccaggtcggc agacgtgacg aagcgattac ggcctaccgg
     1441 aaaagcgccg ccagtgatcc atccaattct attcctctga acaaccttgc atccctgttt
     1501 gcttcggagg cacagtatga cagcgcatta gtgtatctga aaaaagcgta cagcatcaag
     1561 ccgggcgaga tgatggtgat cgaaaacatc gctgccgtca gctacctgga cggtaactac
     1621 ccgcaggcga tggaatttgc cggaaaggcc ttggcgatac aaccgaatgc acaaaggagc
     1681 cttggtgtta tggccgacac ctatcaggcc atgggcaatc ctgcagaggc gatgaagtat
     1741 ctacagcggt tacagcagat acgttgatca aattacccgg atcagcgaaa ttttcgggtt
     1801 cctattcaac gctgtcaaca acccttccgg gttgttcaaa tttttcactt tccctaccgt
     1861 ttccggatcg ttacttttaa gcacttaacc tgaaacggaa aacgaaccga tgaaacttca
     1921 ccattcattc tgcctggcga tcgcctgctg cgtggcgacc gccagctctg cactcaaccc
     1981 ttcgaaagaa tacaaagtga agcccgagaa gtacgggatg aactacaagg aagaaaaagt
     2041 cccgacgaag gacggagcct cgctcaatgc atggttcttc gagaccgcga agaagacgac
     2101 caactggatg gtcatcagcg gcagcggcga cggcaatatg gccgacgaca tcgaactggc
     2161 aggtcagttt ctttcggcgg gctggaacgt tgccttgtac gattatcgcg gctacggcgc
     2221 cagcagcgat tttgccattg acccggatac gtacatctat ccgcaattca tcaatgacct
     2281 gaacgccatg ctcgactatt tgcggaagtc gcgtgccatc accaagttcg acctcttcgg
     2341 caagaatatc ggtgccggac tttcactcgg cgttggcagc aaccgtaccg agacgcgaaa
     2401 gatcatcgcc gatggtccct ggaccggtct ggagcctatg aaaaagaaaa tcaaggataa
     2461 gaagggtaaa gaggtgatca ttccgttcgg ctacgacaag acctatgagc ccatgtacgc
     2521 ctgcgataaa tctcgcccga acatcaaagg cgttctggtc attgtcacgc cacaggacga
     2581 tctgatcaaa cccgaagaca ccaaaacgct ccgttgcgcc acccaaacct acattgtcgc
     2641 caacagtcct tccaatgcgg agaacttcag tacggataag aatgtttatt ttgagaaggt
     2701 gagtaagttt ttgaatcagt gagttagtga atagtgaata gtgaatagtg aatagtgaac
     2761 agtgaacagt aaatagtgaa ttttacctgc ctgccgtagc g
//
LOCUS       DAOTON010000338         2798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28105_length_2798_cov_3.606635, whole genome shotgun sequence.
ACCESSION   DAOTON010000338 DAOTON010000000
VERSION     DAOTON010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28105_length_2798_cov_3.606635"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="P5292_07360"
     CDS             <1..542
                     /locus_tag="P5292_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS38984.1"
                     /translation="AGKSTLLHILGTLDVPDAGTLTIAGEEVSRLKSARLSAFRNRHL
                     GFVFQSHHLLGEFSALENILVPALIAGESRTAAEKRAQELLAQLGLADRAEHRPSEMS
                     GGEQQRVAVARALMNRPDAILADEPSGNLDSASARSLHELFFQLRRDLQQTFIIVTHN
                     EELANMADRKLHMRDGLLH"
     gene            545..1789
                     /locus_tag="P5292_07365"
     CDS             545..1789
                     /locus_tag="P5292_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRS38985.1"
                     /translation="MLLRSLLFLLLLSCSLSAQVDTWQAIADQPIGGRQLAVAFYVDG
                     RGYSGLGTDSLGNYYNDFWAYDPSSDSWTQVADFSGSARKSAVAFALSGKGYVVSGQD
                     TSGLLNDCWSYDPTSNTWSAEQSLGQFSTSSSVGRADATAIETDSLAFILCGYDGGSS
                     WLKQNWQINPRRDTIWTLKRNLANVTESTLFGRRWGAGFSIDNKVYYGCGFSFSQDYR
                     QDVWSYNYLIDAWTQVADFSGGGRSNLCGFSLYDKGYFVGGTNGTTQSDMWRYDPNLN
                     NWTAVASFGGGPVSNAVVFTDDRKAYMGLGRDSAGSSLPRWFVYTPDSTVGVPDVLDH
                     VQLTAYPQPASECVTLSGSYQRTPSSRLELLDQQGRIVLSTAILQWPMLVRTESLPAG
                     MYFYRILDGNTKRGAGKLVVAR"
     gene            1847..>2798
                     /locus_tag="P5292_07370"
     CDS             1847..>2798
                     /locus_tag="P5292_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38986.1"
                     /translation="MITSRLRLLLAVLLLTGLQAGAQVSTYLFVQDSTTYSPINGTVI
                     GDSLTDDQYFVDTASPLGGPLTSGPGIPIGFTFGYAAGNCDVFGISANGWIGIGSGSV
                     NLSNTAPYFPLSTTAGGNIIAGFARNLAAQDGSRLEYATVGTAPDRVLVVQWTNYRKR
                     GNVGDAFNFQIRLYEGSNNIEFVYGPVSCNINSSFVQVGMIGGNSSDFNLRASLGGGG
                     WTGTVSGVLNSATVTLNQSELPPDGLVFRFGAPPPCTAPPIAGNASASAVSVCPNNFV
                     TVTLQNFSSGPGQSYQWESSTDNLNWSPIVGAISTAWNQTL"
BASE COUNT          555 a          861 c          775 g          607 t
ORIGIN      
        1 gtgcggggaa atccaccctc ctgcatatcc ttggcacgtt ggatgttccg gatgccggaa
       61 ccctgacgat tgccggagaa gaagtcagtc gcctgaagag cgcccgattg tccgctttcc
      121 gtaaccggca tctcgggttc gtgtttcaat cccatcactt gctcggggaa ttttccgctt
      181 tggaaaacat cctcgtcccc gccttgattg ccggtgaaag ccgcactgcc gcagagaaac
      241 gagcgcagga actgctcgcg caactcggtc tggccgatcg tgcggaacat cgtccgtcgg
      301 agatgagcgg tggtgaacag caacgcgtcg ccgtagcccg cgccctcatg aaccgacccg
      361 acgccatcct cgccgacgag ccttcgggca atctcgattc cgcatccgcg cgttcgctgc
      421 atgaactctt ctttcagttg cgccgggacc ttcaacaaac ctttatcatc gtcactcaca
      481 acgaagaact ggccaacatg gccgatcgga agttgcacat gcgtgacggc ctgcttcact
      541 gaacatgctg ctgcgttccc tcctattcct gcttctgcta agttgctcac tgtccgccca
      601 ggtcgacacc tggcaggcca tcgccgatca gcccatcggc ggtcgtcagc tcgctgtcgc
      661 tttctatgtc gatggcaggg gctacagcgg actgggtacc gacagcctgg ggaattatta
      721 caacgacttc tgggcatatg acccatcgtc cgacagttgg acccaggtcg ccgatttcag
      781 cggcagtgca cgaaagtcgg cggtcgcttt tgcactgagc gggaagggct atgtggtaag
      841 cggacaggat acctccggct tgttgaacga ctgctggagc tatgatccca catccaacac
      901 ttggtcagcg gaacaaagcc tggggcaatt cagtaccagc agctccgtgg gccgggcgga
      961 tgccacggcg attgaaaccg acagcctggc gttcatcctt tgcggttacg atggcggctc
     1021 ttcctggttg aaacagaact ggcagatcaa cccgcgccgg gatactatct ggacactgaa
     1081 acgcaacctg gccaatgtca ccgagagtac cctcttcggc cgtcgctggg gcgccgggtt
     1141 ctcaatcgac aacaaagtct attatggttg cggcttttcc ttttctcagg attaccgaca
     1201 ggatgtatgg tcgtacaact atctgatcga tgcctggacc caggttgccg atttcagcgg
     1261 cggaggtcga tcgaacctct gcggcttcag cctgtatgac aaaggctact tcgtcggcgg
     1321 cacgaacggt accacacagt ccgacatgtg gcgctacgat cccaacctga ataactggac
     1381 cgctgtagcc agcttcggcg gaggaccggt ttccaatgcc gttgtcttca ccgacgaccg
     1441 caaggcttat atgggactcg ggcgcgactc cgcgggcagc agccttccgc gttggtttgt
     1501 gtacacgccc gattccacgg tgggcgttcc ggacgtactc gatcacgttc agctgacggc
     1561 atatccccag ccggcatccg aatgtgttac cctttccggt tcgtatcaga gaacgccttc
     1621 ctccaggttg gaattgctgg atcagcaagg ccgaatcgtg ctttcgaccg caatactcca
     1681 atggcccatg ctggtccgta cggagtcctt acctgcaggc atgtatttct accgcatcct
     1741 cgatggaaac accaagcgcg gagctggaaa gctggtcgta gctcgctaaa aggcttgccg
     1801 ggaccggaac ttttcgccaa cttgtgggaa ccaccaccca cccaacatga tcacttcccg
     1861 tttgcggctg ttactggccg tgttgctgct gaccggtctg caggccggcg ctcaggtctc
     1921 gacctatctg ttcgttcagg attccaccac ctacagcccg atcaacggga cggtgatcgg
     1981 cgattcactg accgatgacc aatacttcgt tgataccgcc tcaccactgg gtggtccgtt
     2041 gaccagtggc ccgggaatcc cgatcgggtt caccttcggt tacgcggcag gtaattgcga
     2101 tgtgttcggg atcagcgcga acggctggat cggcatcggg tcgggttcgg tgaacttgtc
     2161 caatacggcc ccatacttcc cgctctcgac gaccgcgggc ggcaacatca tcgccggttt
     2221 tgcgcgcaac ctcgcggcgc aggatggctc gcgactggaa tacgccacgg tgggcactgc
     2281 ccccgatcgt gtactggttg tccaatggac caactatcgc aaacgcggca atgtaggcga
     2341 cgcgttcaac ttccagatcc ggttgtacga aggatcgaac aacatcgagt tcgtctatgg
     2401 tccggtgagc tgcaatatca attcgtcctt cgtgcaagtg ggcatgatcg gcggcaacag
     2461 cagcgacttc aacctgcgcg caagtcttgg cggcggcggc tggaccggaa ccgtttccgg
     2521 cgtgctgaac tccgctacgg taaccctgaa tcaaagtgaa ctgccacccg acggactcgt
     2581 tttccgtttc ggagcgccgc ctccctgcac ggcacctccg atagccggca acgcaagcgc
     2641 ttccgccgtt tccgtttgcc cgaacaattt cgtgacagtg accctgcaga atttcagttc
     2701 aggtcccggt cagtcctatc aatgggaatc gagcaccgac aacctcaact ggagtccgat
     2761 cgttggcgct atctccacgg cctggaacca aaccctgt
//
LOCUS       DAOTON010000339         2787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28256_length_2787_cov_3.374817, whole genome shotgun sequence.
ACCESSION   DAOTON010000339 DAOTON010000000
VERSION     DAOTON010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28256_length_2787_cov_3.374817"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..617
                     /locus_tag="P5292_07375"
     CDS             171..617
                     /locus_tag="P5292_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRS38987.1"
                     /translation="MFLLALTSQAQDAAPAPKADNPNAPEITFENETHDYGTIKQGAD
                     GNCEFKFKNTGKEPLIISNAKGSCGCTVPTYPKEPIMKGQTGVIKVHYDTKRVGAFTK
                     TVTINSNGKTDTKVLTIKGVVEASEETSDQAMPFKKTGGMPLENSK"
     gene            642..1094
                     /locus_tag="P5292_07380"
     CDS             642..1094
                     /locus_tag="P5292_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRS38988.1"
                     /translation="MKKITAIFAFLLTLSTTSSFAQTEKKTLDNIGNASNQAEFTFEK
                     DEYAFGTITQGQTVSYDFTFTNTGNEPLIITNAQASCGCTVPQWPKEPIMKGQSGTIK
                     VTFNSTGKLGVQDRTVTITSNAKQNPMVLHLKGTVDKPVEQPAAQPTK"
     gene            1130..1573
                     /locus_tag="P5292_07385"
     CDS             1130..1573
                     /locus_tag="P5292_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRS38989.1"
                     /translation="MKKAILAVSMFLFVAAAQAQTPAAPASSAAALDNKNAADFKFDV
                     EEYNFGSIKQGDKVTYDFNFVNTGKDPLIITEAHGSCGCTVPSYPKEPIAKSAKGTIH
                     VEFNSTGKMGMQDKTITITSNAKSGQKVLHLKGNVEAPEKPAETK"
     gene            1634..>2787
                     /locus_tag="P5292_07390"
     CDS             1634..>2787
                     /locus_tag="P5292_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRS38990.1"
                     /translation="MPKISTKGASMPPSPIRKLVPFAEQAKKAGRTVYHLNIGQPDIE
                     TPDSMLDAIHHFAPKVVEYSHSAGIESYRKKLTTYYKRYQIELDYTDILITTGGSEAI
                     EIAMMSCLDEGDEIIIPEPFYANYNGFSTQAHVKVVPIRSTIESGFALPPVAEFEKLI
                     SPKTRAIMICNPNNPTGYLYSKEELESLRDIVQKHDLWLFSDEVYREFCYDGKQYTSV
                     MHLKGIEQNVVMLDSISKRYSACGARIGALITRNKDLQATALRFAQARLSPPTFGQVA
                     AEAAIDTPDEYFTKVKKEYVARRDFVVSALNQMDGVYCPKPSGAFYCIARLPIDNADK
                     FCQWLLESFSWQNQTVMLAPATGFYSRPDLGTDEVRIAYVLNLDSLKNAM"
BASE COUNT          803 a          801 c          610 g          573 t
ORIGIN      
        1 tatcaccgct gttcagtaca aaattagcca ttccctcgtt catactgact tcattgcagc
       61 ctgattactt ttggcaaagg atttgcaata agaccgcgcc aaagcctcaa tccgtatctt
      121 tgcgtaaacc cgctatcact atgaaaaaaa taactacttc gctgtgcatc atgttcttac
      181 tggcgctgac cagccaggcg caggatgcag ctccagcccc caaagccgat aaccctaacg
      241 caccggaaat cacgtttgaa aatgaaacac acgattacgg caccatcaaa cagggagcgg
      301 acggcaattg tgagttcaag ttcaaaaata cgggcaaaga acctttgatc atctccaacg
      361 ccaagggaag ttgcggctgc accgtaccta cctacccgaa agagccgatc atgaaaggac
      421 aaaccggggt gatcaaggtt cattacgaca ccaaacgcgt cggcgccttc acgaaaacgg
      481 taaccatcaa ctcgaacgga aaaacggata ccaaagtcct gaccataaaa ggagttgttg
      541 aagcttctga agaaacttcc gatcaggcaa tgccgttcaa gaagaccggc ggtatgccgc
      601 ttgaaaactc gaaataattc gatcaaccct cctcatccac tatgaaaaag atcaccgcaa
      661 tttttgcttt cctgctgaca ttgtcgacca cttccagctt cgctcaaacc gaaaagaaga
      721 cgctcgacaa catcggtaac gcatccaatc aggccgaatt cacctttgag aaggacgaat
      781 acgcctttgg tacgatcacg cagggtcaaa ccgtttccta cgatttcacc ttcaccaaca
      841 ccggaaacga acccctcatc attaccaacg cccaggcatc ctgcggctgt acggttccgc
      901 agtggccgaa agaaccgatc atgaaaggac agagtggcac gatcaaggtc acgttcaact
      961 cgaccggcaa actgggagtg caggatcgca cggtaaccat cacctccaac gcgaagcaaa
     1021 atccgatggt gttgcatttg aaaggcaccg tggacaaacc ggtggaacaa cctgccgcac
     1081 aaccgacgaa ataagttcac cctaaaccaa ccacataatt aaacaaccaa tgaaaaaagc
     1141 gatcctggca gtcagcatgt tcctgttcgt ggcggctgct caagcacaaa ctccggcagc
     1201 tccggcatct tccgctgcag ctctcgacaa caagaacgcg gctgacttca aattcgacgt
     1261 ggaagagtac aacttcggct ccatcaagca aggagataag gttacctacg acttcaactt
     1321 cgtcaatacc ggtaaggatc ctttgatcat caccgaagcg cacggctcct gtggttgcac
     1381 cgtaccttct tatccgaaag agccgatcgc aaaaagcgcg aaaggaacca tccatgtcga
     1441 gttcaactcg accggcaaaa tgggtatgca agacaagacc attaccatca cctccaatgc
     1501 caaaagcgga caaaaggtgt tgcaccttaa aggaaacgtc gaagcaccgg aaaaaccggc
     1561 tgagaccaag taatcaggga aattcctaat tttaagccct cccacaagga gggctttttt
     1621 atttcccgct tccatgccga agatctcaac gaaaggcgca tccatgccgc cttctcctat
     1681 ccggaaactg gtaccctttg ctgaacaagc taaaaaagca gggcgcaccg tctatcatct
     1741 aaacatcggt caaccggaca tcgagacgcc ggattccatg ttggatgcca ttcatcattt
     1801 cgcgccgaaa gtcgtcgaat acagccactc tgccggcatt gagtcctacc ggaagaaact
     1861 cactacctac tacaagcggt accagattga actcgactac accgacatcc tgatcaccac
     1921 cggaggttcg gaggccatcg agatcgccat gatgagctgt ctcgacgaag gcgatgagat
     1981 catcattccg gaacccttct atgccaacta caacggattt tccacccagg cacatgtcaa
     2041 ggtagtaccc atccgttcaa ccatcgaaag cggcttcgcc cttcctccag ttgccgagtt
     2101 cgagaaactg atcagcccga aaacacgggc aatcatgatc tgcaatccga acaatccgac
     2161 cgggtacctc tactcgaagg aagaactcga atcgctacgg gacatcgtgc aaaagcacga
     2221 cctctggctg ttctccgatg aggtctaccg tgaattctgt tacgacggca aacaatacac
     2281 atccgtcatg cacctgaaag gcatcgagca gaacgtggtc atgcttgatt ccatttcgaa
     2341 acgctacagc gcctgtggtg ccagaatcgg agccctgatc acccgcaaca aggaccttca
     2401 ggctaccgcc cttcggttcg cccaggcacg tctcagtcct cctacatttg gacaagtggc
     2461 tgccgaggcg gcaattgaca caccggacga atattttacc aaagtcaaaa aggaatacgt
     2521 tgcccgacgg gattttgtag tcagcgcgct caaccagatg gacggcgtat actgccccaa
     2581 accgagtgga gcattttact gcatcgcacg tcttcctatc gacaacgcag acaagttttg
     2641 tcaatggtta ctggaatcat tttcctggca gaatcagacc gttatgctgg cgcctgccac
     2701 cggcttctac tctcgtcccg atttgggtac agacgaagtt cgtatcgctt acgtactcaa
     2761 tctggactca ctgaagaatg ccatgaa
//
LOCUS       DAOTON010000340         2785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28280_length_2785_cov_3.730037, whole genome shotgun sequence.
ACCESSION   DAOTON010000340 DAOTON010000000
VERSION     DAOTON010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28280_length_2785_cov_3.730037"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1977)
                     /locus_tag="P5292_07395"
     CDS             complement(<1..1977)
                     /locus_tag="P5292_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRS38991.1"
                     /translation="MNKFFLLAFSLLASLLLRADNPPIGLGSWREHIPYNKAVAVADA
                     GDRVYCASQFGLFSYKKDDGEISYFTRLGGLSDYEIKTIRYETQRGILVIAYENSNID
                     LLFADKTIVNLSDIKRKNIVGGKAINHILFINGKAYLSCQFGIVVINLDRREVEDTYF
                     IGPNGGNLNVQGLAYDGTQLLAVSDSGVFLANFNDPNLFNYTAWNRESSLAEPLGNYT
                     STCCFRGKFYVVKTNEDRDKDSVLVRDSGQWRPFFSEASEGAAIDEYNDLFMYRNYYK
                     LVALDEQTNQVRGMDVNAVGNSEFRGGQVDAAGNFWLADYTNGLIRERPGPQISVITQ
                     NGPRSSAVWDMDSKGGALWVASGSLNGDAPNYNQVNGVYLFDKNEWKTFDKRNDPIYN
                     TINSPAVITVSVDPSNREHAYCGVWGGGLLEYGPNGAVTRYTESNSSLLSIAGLNNYI
                     ITGGTAFDEDGQLWVVAGGNTTPLSVRKTDGSWQNFRIPDADVAGYGLYDLCVDDFGQ
                     KWFIAREGASAGRGLCVYAENDMANPNDNQFRRLTDASGNGSLPDVFVRCIANDQDGA
                     LWIGTNKGVAVIYNPGSVFSSTSFDAQKVIIQQDGYNQYLLESEYVTAIAIDGANRKW
                     FGTYSGGAFLMSADGTKQLLNFNTGNSPLASNTIL"
     gene            2072..>2785
                     /locus_tag="P5292_07400"
     CDS             2072..>2785
                     /locus_tag="P5292_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS38992.1"
                     /translation="MDKLPFSALDRRLLSLEQRWSLPVLALILGAAVLALSALGTSPQ
                     FVPVHHGKWYTVLSNNPFDWSDNYNLRFRILAPFLGYILFLRGPLFPWFMLSLLAVFL
                     GMVYRYLRREDWRPVDALSLLLLFGFSTLTFYTYSFPGYTDSFTYVLLTLAWFAPKKH
                     GLRVLLLALLIFNHEQTIFLFPLLFLRWRPESWQANDWLKHVGWFVLALVPYFVYREI
                     VHRISPVEYTVGYYLDPANL"
BASE COUNT          483 a          826 c          819 g          657 t
ORIGIN      
        1 gaggatggtg ttcgaagcaa gcggggagtt acccgtgttg aagttgagca gttgtttggt
       61 gccgtcggcc gacatcagga acgcgccacc gctgtacgtg ccgaaccact tgcggttggc
      121 gccgtcgatc gcgatcgcgg tcacgtactc cgattcgagc agatactgat tgtagccgtc
      181 ctgctggatg atcaccttct gcgcgtcgaa gctcgtcgag ctgaacacgc tgcccggatt
      241 gtagatcacc gccacgccct tgttggtacc gatccacaac gcgccgtcct ggtcgttggc
      301 gatgcagcgc acgaacacgt cgggcaggga gccgtttccg ctcgcgtcgg tcagtcgccg
      361 gaattggttg tcgttcggat tggccatgtc gttctcggca tacacacaga gaccgcgacc
      421 ggcagaagcg ccttcgcgcg cgatgaacca cttttgtccg aagtcgtcga cgcagaggtc
      481 gtacaagccg tatccggcaa cgtcggcgtc cggaatgcgg aaattctgcc agctcccgtc
      541 cgtcttgcgc acgctcaggg gagtcgtgtt gccgcctgcg accacccaca gttgtccgtc
      601 ttcatcgaaa gccgttccgc cggtgatgat gtaattgttg agtccggcga tcgagaggag
      661 ggaagagttg ctctcggtgt agcgtgtcac cgcgccgttc ggaccgtact ccagcaaccc
      721 accgccccag acaccgcagt aggcgtgttc ccggttgctc ggatcaacgg agacggtgat
      781 gaccgcggga gaattgatcg tattgtagat tgggtcgttc cgtttgtcaa acgtcttcca
      841 ttcgttctta tcgaagaggt acacgccgtt cacctggttg tagttcggcg cgtcgccgtt
      901 gagtgaaccc gaggccaccc aaagggcacc gcctttactg tccatgtccc agacggccga
      961 ggagcgcgga ccgttctggg tgatgacgct gatctgcggg cccggtcgtt cccggataag
     1021 accgttcgta taatcggcca gccagaaatt tccggccgcg tctacctgtc cgccccggaa
     1081 ctcactgttc ccgacggcgt tcacatccat gccgcgtacc tggttggtct gttcgtcgag
     1141 cgcgaccagc ttgtaatagt tccggtacat gaacaggtcg ttgtattcgt cgatcgccgc
     1201 gccttccgag gcttcggaaa agaacggccg ccattgtccg ctgtcgcgta caaggacact
     1261 gtctttgtcg cggtcttcgt tcgttttcac gacatagaac ttcccgcgaa aacagcaggt
     1321 cgaggtgtag ttgcccagcg gctcggccag tgaactctca cggttccagg cggtgtaatt
     1381 gaacaggttc ggatcgttga agttggccag gaacaccccg ctgtcgctga ccgcgagcag
     1441 ttgggtaccg tcgtacgcga gtccctgcac gttcaggttg ccgccgttcg gcccgatgaa
     1501 gtaggtgtct tccacttcgc gccggtcgag gttaattact acgatcccga actgacagct
     1561 caggtaggct ttgccgttga tgaacaggat gtggttgatc gcctttccgc cgacgatgtt
     1621 cttgcgcttg atgtcgctca ggttgacgat cgtcttgtcg gcaaacagca ggtcgatgtt
     1681 cgaattctcg taggcgatca ccaggatgcc gcgctgggtt tcgtaccgga tcgttttgat
     1741 ctcgtagtcg gaaagtccgc ccaggcgcgt gaaatagctg atctctccgt cgtctttctt
     1801 gtaactgaac agtccgaact ggctggcgca atacacccgg tcgcccgcgt cggcgacggc
     1861 aacggccttg ttgtagggaa tatgttcccg ccagctaccg agcccgatcg gcgggttgtc
     1921 ggcacgcagt agcagggaag caaggaggga aaaggcgagg aggaagaatt tgttcatctt
     1981 tgcaggcgat cgtagcaaaa cggtccgcag gcaccgctta ttttacccgg gcgggccgga
     2041 ccgaaagtta cgaatcttcc cgcccgcttc cgtggacaag ctgcctttct ccgccctcga
     2101 ccgtcgcttg ctttcgttgg agcagcgctg gtcccttcct gttctggcgt tgatcctggg
     2161 cgccgccgtg ctggccctgt ctgcgctggg caccagtccg caattcgtac cggtccacca
     2221 tggtaaatgg tacacggtat tgtccaacaa ccccttcgac tggtccgaca actacaacct
     2281 gcgcttccgg atcctggcgc cgttcctggg ctatatcctg ttcctgcgcg gacccttgtt
     2341 cccctggttc atgctgtcgt tgctggcggt gttcctgggt atggtttatc gctacctacg
     2401 acgggaagac tggcgaccgg tcgatgccct gagcctgctg ctcttgttcg gcttctcgac
     2461 gctcacgttt tacacctaca gttttccggg atacacggac agcttcacct atgtgctgct
     2521 gaccctcgcc tggttcgctc cgaagaaaca cgggttgcgg gtactgctgc ttgcgttgct
     2581 gatcttcaac cacgagcaga cgatcttcct tttcccgctc ctgttccttc gctggcgacc
     2641 ggaaagctgg caggcaaacg actggctgaa gcacgtggga tggttcgtgc tcgccctggt
     2701 cccgtacttt gtttatcgcg agatcgtgca ccgtatttcg ccggtcgagt atacggtggg
     2761 ttactacctc gatccggcca acctg
//
LOCUS       DAOTON010000341         2784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28294_length_2784_cov_2.915354, whole genome shotgun sequence.
ACCESSION   DAOTON010000341 DAOTON010000000
VERSION     DAOTON010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28294_length_2784_cov_2.915354"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="P5292_07405"
     CDS             <1..452
                     /locus_tag="P5292_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRS38993.1"
                     /translation="KEIIGKAWPVAIAQLTDLYGEDQDNWHWLRAHTLEIGHPLAKQP
                     PLQLVFGIGSYPIPGGMETINNQSFTLTGRGSYSVNLGPALRRIVDFAQPESGESINP
                     SGQSGNPSSAYYQDQADRYASGKFREELLDRKRIEAETKHTLIFQPK"
     gene            532..603
                     /locus_tag="P5292_07410"
     tRNA            532..603
                     /locus_tag="P5292_07410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:565..567,aa:Arg,seq:ccg)
     gene            complement(687..1814)
                     /locus_tag="P5292_07415"
     CDS             complement(687..1814)
                     /locus_tag="P5292_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRS38994.1"
                     /translation="MKSFSSCADFTMEQYSYYDKLIDQTPDVFCILRGQGQIIYCGSS
                     SRKLLGYDPEAIVGRNALRYLHPAERHQARLRHSVLLAYPGNRVTADYRVLDAHGEYR
                     WMECIFNNMLNLPQIDGILVQLRDVTDRKNFELQLEESEERFRIFMNNAPTVSMIKDE
                     EGRLVFVNANFERSFRVLSKDALGMTLGELFPNGYWAESEENDRIVLNFGKSVEFFER
                     TITPDGVEREWIVYKFPVPQSNGKTFIGCSAMDITKWSSLDRERVTAEQKFKTIFEYS
                     PDPIFIENEDGIIIDANRKACELQGMNYPELVGKRITDLAPPDRHEELWSDFSKLWNG
                     QLSRIDSYSWSTSGQVIPVAISTARIQHMDQPCLLLALRER"
     gene            1967..2425
                     /locus_tag="P5292_07420"
     CDS             1967..2425
                     /locus_tag="P5292_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013868694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRS38995.1"
                     /translation="MENSIWINVLAFLTSYFAMEGVAWATHKYVMHGFLWTLHESHHQ
                     PRHGVFERNDFFFLFYATIAMVLMYFGWEQLDFRFWMGLGVTAYGFTYFMLHDVFIHR
                     RGKWLDKLDNSYFRAMRKAHKVHHKKMGKEDGEEFGLLLFNKRHWRVNQD"
     gene            2478..>2784
                     /locus_tag="P5292_07425"
     CDS             2478..>2784
                     /locus_tag="P5292_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRS38996.1"
                     /translation="MSKKVLIIGTGLGGLATALRLACRGYQVEMVEKHGRAGGRLNQM
                     EKDGFTFDVGPSFFSMSYEFTELFRDCGIPNPLELEELNPVYSVNFAHRKKPFLIYKD
                     "
BASE COUNT          629 a          697 c          789 g          669 t
ORIGIN      
        1 gaaaagagat catcggcaaa gcctggccgg tggccattgc gcagttgacg gacctctacg
       61 gtgaagacca ggacaattgg cactggctgc gcgcgcatac gctcgagatc gggcatccgt
      121 tggcgaagca gccgcccttg caattggtct ttggaatcgg ttcgtatccg ataccgggtg
      181 ggatggagac gatcaacaac cagagtttta cgctgactgg aaggggttcg tattcggtga
      241 atctcggacc ggcgctgcgc cgcattgtgg acttcgcgca gccggagtcg ggtgagagca
      301 tcaatccgag cggacagtcc ggcaatccgt cgagcgcgta ttatcaggac caggccgacc
      361 ggtatgcctc gggaaaattc cgggaagagc tactcgaccg aaaacggatc gaagcggaga
      421 cgaagcacac cctgatcttc caacccaagt gaggcctcga tcccgctgcc gaaaggttga
      481 tgattcgccg gaaggatgtt acctttgcaa aacggttgaa aaaccgccat tggtctcata
      541 gtacaatgga tagtatagag gcctccgaag cctttgatct aggttcgact cctagtgaga
      601 ccgcagattc aagcaactga ttatcaacaa gaaagctccg aaaaatcgga gctttttgtt
      661 tttacaggta gttctcgatt cccggctcag cgttcgcgca acgcgagcaa aagacagggt
      721 tgatccatgt gttggatacg tgctgtgctg atggcgaccg gaatgacctg gccgctggtg
      781 gaccaggagt agctgtcgat ccttgacaat tgtccgttcc agagcttgct gaaatcgctc
      841 cacaattcct cgtggcggtc cggcggtgcg agatcggtga tgcgtttgcc tacgagttcg
      901 gggtagttca tgccctgcag ttcgcaggcc ttgcggttgg cgtcgatgat gattccgtct
      961 tcgttttcga tgaagatcgg atcggggctg tattcgaaga tcgttttgaa cttctgctcg
     1021 gcggtgacgc gttcgcggtc gaggctggac catttggtga tgtccatggc cgaacaaccg
     1081 atgaaggtct tgccgttcga ttggggcacc ggaaatttgt acacgatcca ctcacgctct
     1141 acaccgtcag gcgtaatggt ccgctcgaag aactcgacgg actttccaaa gttcaggacg
     1201 atgcgatcgt tctcttcgga ttcggcccag tagccgttcg gaaagagttc tccgagcgtc
     1261 atgcccagcg cgtctttcga caacacacgg aaggaacgtt caaagttggc attgacgaag
     1321 accaggcgtc cttcttcatc cttgatcatc gagaccgtgg gcgcgttgtt catgaagatg
     1381 cggaaacgct cttcggattc ttccagttga agttcgaagt tcttgcggtc ggtgacatcg
     1441 cgcagttgga cgaggatgcc gtcgatctgc gggaggttga gcatgttgtt gaagatgcac
     1501 tccatccaac gatactcgcc gtgcgcgtcc aggacgcggt aatcggcggt aacccggttg
     1561 ccgggatagg ccaggagtac ggaatggcgc agccgtgcct gatgacgttc ggcgggatgc
     1621 aggtagcgca aggcattgcg ccccacgatc gcctcaggat cgtaacccag cagttttcgc
     1681 gaacttgatc cgcaataaat gatctgtccc tgaccacgca ggatgcaaaa gacatccggt
     1741 gtctggtcga tcagcttgtc gtaatacgaa tattgctcca tggtaaagtc cgcgcaggat
     1801 gaaaaggact tcacgccgtg aaaataccaa gtccctgaac ttaagtacgc gtcttttgtt
     1861 agagttcatc agtcaagttg tcaacaggct tggtcgttgt atattcaata attatttgaa
     1921 tttcaattaa ttactgttgt gcaacgagaa gccgtaactt cgcagcatgg aaaacagcat
     1981 ttggatcaat gtccttgcct tcctgacatc ctatttcgcc atggaagggg tagcctgggc
     2041 tacgcataaa tatgtgatgc atggttttct ttggaccctg cacgagtcgc atcaccaacc
     2101 gcgtcacggc gtgtttgagc ggaatgattt ctttttcctc ttctacgcga cgatcgcgat
     2161 ggtgttgatg tatttcggct gggagcaact cgattttcga ttctggatgg gacttggcgt
     2221 gaccgcgtac ggcttcacct acttcatgct gcacgatgtg ttcattcatc gtcggggcaa
     2281 gtggctggac aaactggaca attcctattt ccgcgccatg cgcaaagcgc acaaggtgca
     2341 tcataagaaa atgggcaagg aagacggcga agagttcggc ttgttgctct ttaacaaacg
     2401 tcattggcgc gtcaaccagg attgaacaat tccgcaccaa ccttgttgcc ctacctgttc
     2461 accaaaacgc ctgaaccatg tcgaaaaaag tactgatcat cggaaccggt ctgggtggat
     2521 tggctacggc cttgcggctg gcttgccggg gttatcaggt ggagatggta gaaaagcacg
     2581 ggcgtgccgg tggtcgcctg aaccagatgg aaaaggacgg attcaccttc gatgtgggtc
     2641 cttcgttctt cagcatgagt tacgagttca cggagttgtt ccgcgactgc gggattccca
     2701 atccgctcga gctggaagag ctcaaccccg tttattccgt caacttcgct caccggaaaa
     2761 agccgtttct catctataag gatg
//
LOCUS       DAOTON010000342         2780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28347_length_2780_cov_2.222752, whole genome shotgun sequence.
ACCESSION   DAOTON010000342 DAOTON010000000
VERSION     DAOTON010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28347_length_2780_cov_2.222752"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1255)
                     /locus_tag="P5292_07430"
     CDS             complement(191..1255)
                     /locus_tag="P5292_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRS38997.1"
                     /translation="MIKLLTIILLVLAVITVWRVLRILELVRELRGEEEYITDADNRN
                     TGRGLIAFLILGMLGMIYFTLDARKYLLPQAASKHGVLTDDYLYVNFGIIIVVFFITQ
                     ILLFVFANKYRYRKDQKAFYYPDNHKLELIWTVVPGVVLMGLIAYGLHLWINITAPAP
                     KEAMVIEVYAKQFDWTARYSGADNKLGNSNFRWITDENPLGVDNKDQASGDDKITREI
                     HLPAYTPVVFKFHSRDVIHSAYFPHLRAQMNCVPGMTTEFHLEPTISTDSMRIITNNP
                     KFEYVLLCNKICGVAHYNMRMKVVIETPEAFKKWYKEQQYVFARPEEAAPAATPATPV
                     DSSAGKTAVIAQNPKIIASR"
     gene            complement(1294..2475)
                     /locus_tag="P5292_07435"
     CDS             complement(1294..2475)
                     /locus_tag="P5292_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HRS38998.1"
                     /translation="MTDRKYELTQNNKVLPLVLMAIGVAAVAVGFMTDKTRAWASLLT
                     NGFYFNAIALAGTFFVAVNYVAQAGWAVGIKRVAEAMGGFLKFSMAILIITFLGGHHD
                     LYHWTHHELYDPNSPEYDAILAGKSGYLNMTFYIIRLVAYAAIWVGFTYMLRKNSLQE
                     DINGGLSYYNSSVVVSAIFIVLFAITSSTSGWDLLMSVDSHWFSTLFGWYTFAGLFVS
                     GMSMMALLTLYLKGRGYMQHVNDNHIHDVGKFMFAFSVFWTYLWFSQFMLIWYANLPE
                     EVVYYKVRWENFRSLWILNLVINFIAPFLVLMTRDAKRRFGIVWVAGIIILCGHWLDV
                     FLMVMPGTVGTNWHIGFIEVGTGLGFLGLFLWSTFRELSKAALVPKNHPMLPETLHHH
                     I"
     gene            complement(2500..>2780)
                     /locus_tag="P5292_07440"
     CDS             complement(2500..>2780)
                     /locus_tag="P5292_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRS38999.1"
                     /translation="VCHGTSGQGDGPVPSNNGPKPPAYNSDLLKTLPEGKMFHSIHYG
                     KNMMGSHASQLTQSQRWMIVQYVQTLQHYGEAAAAPKDSVQTTASATK"
BASE COUNT          634 a          708 c          821 g          617 t
ORIGIN      
        1 gatcatcttg tgatcctggc tgaacacgta tttggtccag aaagaatcgt gatgctcgtg
       61 gtgatcgtgc ccctcgtggt gagcgtcgtg ggcatgatcg tgtgcgtgcg tcgacatgct
      121 atggtttggg attattcgtt actattctaa atgaaagttc cgcattcagg accgaagccg
      181 ttgtggcttc ttatctggat gcaataattt tcgggttttg agcgatgaca gcggtcttgc
      241 cggcggacga atcaaccggg gtagcggggg tcgctgccgg cgcggcttcc tccggacggg
      301 caaacacgta ttgctgttcc ttgtaccact tcttgaacgc ctccggagtc tcgatcacca
      361 ctttcatgcg catgttatag tgcgccaccc cgcagatctt gttgcagagc aggacatatt
      421 caaacttcgg attgttggtg atgatccgca tgctgtcggt cgagatcgtc ggctccagat
      481 ggaactcggt ggtcataccc ggcacacagt tcatctgggc gcgcaggtgc gggaagtagg
      541 ccgagtggat gacatcgcgg gaatggaact tgaagaccac cggcgtataa gccggcaggt
      601 ggatctcccg ggtgatcttg tcgtctccgg aagcctggtc cttgttgtct acacccagcg
      661 gattctcatc cgtgatccac cggaagttcg aattgccgag tttattgtca gcacctgaat
      721 aacgtgcggt ccagtcgaat tgtttggcat agacctcgat caccatcgcc tctttgggag
      781 ccggcgcggt gatgttgatc cagaggtgca gaccgtacgc gataaggccc atcaggacca
      841 cgcccggtac caccgtccag atgagttcca gtttgtgatt gtccggatag taaaaagcct
      901 tctggtcctt acggtaacga tacttgttgg cgaaaacgaa taacaggatc tgggtgatga
      961 agaacaccac gatgatgatc ccgaaattca catagagata gtcatcggtc agtacgccgt
     1021 gcttggacgc tgcctggggc aagaggtact tcctggcatc cagggtgaag tagatcatgc
     1081 ccagcatacc caggatcagg aacgcgatca gcccgcgtcc ggtgttccgg ttgtcggcat
     1141 cggttatata ttcctcttca ccgcgcagtt cccggaccag ttcaaggatc cggagcacac
     1201 gccagaccgt aatgacggcc agcacgagga ggatgattgt cagcagcttt atcatggatg
     1261 cgaattaacc gtgtgaatga atgaagtgct cgatcagatg tggtgatgca gcgtttccgg
     1321 caacatcggg tgatttttcg gaacgagtgc ggctttcgag agctcccgga aggtgctcca
     1381 gaggaagagg ccgaggaatc caaggccggt accgacttca atgaagccga tgtgccaatt
     1441 ggttcctacc gttcccggca tgaccatcag gaacacatcc agccagtgtc cgcaaaggat
     1501 gatgataccg gcaacccaaa cgatgccgaa acgacgcttg gcgtcgcgtg tcatcagcac
     1561 caggaaaggc gcgatgaagt tgatgaccag gttgaggatc cagagtgagc ggaaattctc
     1621 ccagcgaact ttgtaataca cgacttcttc cggaaggttg gcgtaccaga tcagcatgaa
     1681 ctgggagaac cagaggtagg tccagaacac cgagaacgcg aacatgaact tacccacatc
     1741 gtggatgtgg ttgtcgttca cgtgctgcat ataaccgcgg cctttcaggt agagggtcag
     1801 cagggccatc atggacatgc cgctcacgaa caaacctgcg aaggtgtacc agccgaagag
     1861 cgtggagaac caatgactgt caacgctcat caacaaatcc caaccggaag tggaagaggt
     1921 gatggcgaag agtacgatga aaatcgcgga aacgacgaca ctgctgttgt aatacgacag
     1981 tccgccgttg atgtcctcct gcagggagtt cttgcgcagc atgtacgtga aaccaaccca
     2041 gatcgccgcg taggcgacca ggcggatgat gtagaacgtc atgttgaggt aaccgctctt
     2101 tccggccagg atcgcgtcat actccggact gttcggatcg tacagttcgt ggtgcgtcca
     2161 gtggtaaaga tcgtgatgac cacccaggaa ggtgatgatc aggatggcca tgctgaactt
     2221 gaggaatccg cccatggctt ccgccacgcg tttgattcca acggcccaac ctgcctgcgc
     2281 gacgtagttg acggcgacga agaaggtgcc ggccagcgcg atggcgttga agtagaaacc
     2341 gttggtcagg agcgaagccc aggcgcgggt cttgtccgtc atgaaaccga cagccacggc
     2401 agcaacaccg atggccatga ggaccagcgg gaggactttg ttgttctgcg tgagctcgta
     2461 ttttctatcc gtcatagcgt tcggaattgt cgtagtggat tacttggttg cggatgccgt
     2521 ggtctgaacg gaatccttgg gcgccgccgc agcctcgccg taatgttgaa gcgtctgaac
     2581 gtactgcacg atcatccagc gctgcgactg ggtgagttgg gatgcgtgcg aacccatcat
     2641 gttcttaccg taatggatcg aatggaacat ttttccttcg ggcagcgtct tcagcagatc
     2701 ggaattataa gccggaggct tcggaccgtt gttcgacgga acaggaccat cgccctggcc
     2761 ggaggttccg tggcagacga
//
LOCUS       DAOTON010000343         2776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28398_length_2776_cov_2.639471, whole genome shotgun sequence.
ACCESSION   DAOTON010000343 DAOTON010000000
VERSION     DAOTON010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28398_length_2776_cov_2.639471"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="P5292_07445"
     CDS             complement(<1..711)
                     /locus_tag="P5292_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39000.1"
                     /translation="MKRESSYLPMLLLLACIFALPAFGQQDIKTVRTSWVDSLKIMGQ
                     MTGKEQYVNYSAQPISMPASNQRGVVIPNQTTATSCNCWLPRDATWQIGQFDGSGGSG
                     GPGVAPEYRNDDWTTPIINLPFNFCFYGTTVTQVYLNNNGNVSIGAPYSTFTANTFPD
                     PTYTMIAPFWGDVDTRAAGSGLVYYQLTATHLVVQWESVGYFGNHDDLLNTFQLIMTD
                     GSDPLLPPGNNVQFCYRDM"
     gene            1226..1489
                     /locus_tag="P5292_07450"
     CDS             1226..1489
                     /locus_tag="P5292_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39001.1"
                     /translation="MSDKTVDIMLSSLRDFAGVTKLEACYELQVVIIAYDAERIDSEE
                     RLLSIIRSLELNSPIELKNAADIPKIRSAYEITVHPIQPTTAR"
     gene            1498..>2776
                     /locus_tag="P5292_07455"
     CDS             1498..>2776
                     /locus_tag="P5292_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39002.1"
                     /translation="MRRTIRHILLIGLTLLLLSGAERALAQSNNLCPTGSVPSLTVNN
                     CAGVTDYTVAQSFTVDNTANVASATVCVSTANNRRDGWYRFTATSTSSTIEVTVAQGT
                     RNIAVAVYTGTCGAMTEVGCVNNAGNGGTETITLTTTIGTTYYIRLIRINSGSTGNDI
                     DGTIAVRSPLGNDNCGGAIVLTPGAPGAACSSVCGTTFGATGSTPATACSGTADDDVW
                     YSFTATQISHSITVDGTGNFDPVVALLSGGCGALTNQACSDLTGNGGVETLVFNNLVA
                     GQTYFIRVYDYDNGAPSDYEFTICVTTPILPTCPGSMGLGVVNVASLPYSSTGRTTTG
                     RGNEFTASNTVICGNSNYYQSNDEVFVFTPATSGLVSIAVTSGSSNVGIMLYNGCPFI
                     GQGGTCVDYSQSTSGNQFLCVNVVAGNTYYLLVD"
BASE COUNT          683 a          705 c          683 g          705 t
ORIGIN      
        1 catgtcacgg tagcagaact gaacgttatt tcccggaggc agcaaaggat cactgccatc
       61 ggtcatgatc agttggaagg tgttgagtaa gtcgtcgtga ttaccgaagt aaccgacact
      121 ttcccattgc accacgaggt gagtagccgt gagctgatag taaaccaacc cgctacctgc
      181 tgcgcgggta tcaacgtcac cccagaacgg agcgatcatg gtgtaggtcg gatccggaaa
      241 agtgtttgcg gtaaaagtgg agtaaggcgc gccgatggag acgttaccgt tattgttcaa
      301 atagacttgt gtgaccgtgg taccgtaaaa gcaaaaattg aatggaaggt tgatgatcgg
      361 ggtggtccag tcgtcgtttc gatattccgg tgcaacaccg ggaccgccac tgcctccgct
      421 tccgtcgaat tgaccgatct gccaggtagc atcccggggg agccagcaat tacaggaagt
      481 agccgttgtc tgattcggga tgactacacc ccgctggttc gacgcgggca tgctgatcgg
      541 ttgcgcactg tagttgacat actgttcctt tccggtcatc tgtcccatga tctttaaact
      601 gtcgacccag gatgtgcgaa ccgttttaat gtcctgttgt ccgaaagccg gcaatgcaaa
      661 aatgcaggcc aacagcaaca gcattggtaa gtaggaactt tcccttttca tggatgattt
      721 taatgaatgg cacaaccgca cacctgacga atgccgcccg tgcgaagtcc ttttcaaata
      781 tatgaaatac aaggggtaag gaagtgaagg aaatactgat tggtaagtaa atccggttag
      841 catcaactct tccttctatg aggagaataa ggttggagtt gcatcaaggc tggatttatt
      901 attggcctta attccccatg tgactgaagt cgtttaccaa gtaaaacata cattttacca
      961 tttataatat caaattaaca ttttgagcat aaacatgctg tatatttgca aagtaccctc
     1021 tctgaattat tgatccattt taatcccata ctaattgtaa aaaccttaaa atcaaacaaa
     1081 tatgagcgcc tgtgcaactc tgcagcacag ggtcttggtt ttatcctgtt tttgcttggt
     1141 tttattcgga attgcttcga aagtccaggc acaagcccct gtaattgaca acacccgaaa
     1201 ggagctgttg ctctccttac cgttaatgtc tgacaaaacc gtcgacatca tgttaagctc
     1261 ccttcgggac tttgccggag taacgaagtt ggaagcctgt tatgagctgc aagttgtgat
     1321 cattgcatac gatgcggaac gaatcgattc cgaagaacgt cttttgtcca tcattcgaag
     1381 ccttgaactc aattctccca tcgagctcaa gaacgctgct gatataccga agatcagaag
     1441 tgcatacgaa atcactgttc atccgataca accaacgacc gcccgctgat acaattcatg
     1501 cgacgaacga tacgccatat cttattgatc gggttaacgc tgctgctact ttctggcgcg
     1561 gagcgcgctt tggcgcagtc aaataacctt tgtccgaccg gtagtgttcc cagtttaaca
     1621 gtgaacaatt gtgccggtgt cacggattac acggttgcac agtcctttac ggttgataat
     1681 acggcgaatg tcgcatcagc gacagtttgc gtatcgacag cgaataaccg ccgtgacggt
     1741 tggtatcgat ttactgcgac gtcgacctcc tcgaccatag aagtcacggt agcccaggga
     1801 acccgcaaca tcgctgttgc ggtgtacacc ggaacatgcg gcgccatgac ggaagttgga
     1861 tgtgtcaaca atgccggtaa cggcggtacc gagaccatta cgctcactac gactatcgga
     1921 actacttatt atatccgatt gatccggatc aactccggca gtaccggaaa cgatatcgat
     1981 ggtacgattg ccgtccgttc accactcggc aatgataatt gtggtggcgc catcgtgctc
     2041 acgccgggcg cacccggggc cgcctgttca tccgtatgcg ggactacctt cggcgcaacc
     2101 ggatcaacgc ccgctacggc ctgttccggc actgcggatg acgacgtttg gtacagtttt
     2161 accgcgacgc agatcagcca tagcattacc gtcgatggaa ccggtaactt cgatcctgta
     2221 gtcgccctgc tttccggcgg atgtggcgct ttgaccaatc aggcgtgttc cgacctgacg
     2281 ggaaacgggg gcgtcgaaac actcgtcttt aataatctgg tcgccggcca aacgtacttt
     2341 atccgggttt atgattatga taacggcgcc ccatccgatt acgaattcac gatctgtgtc
     2401 acgactccca tcctcccaac ctgccccggt tcgatggggt tgggcgtagt caacgttgca
     2461 tcccttccgt attcgtccac cggtcgtacc acgaccggtc gcggtaacga gttcaccgcg
     2521 agcaataccg ttatttgtgg aaactcgaat tactatcaat ccaacgacga agtttttgtc
     2581 tttactcctg caacttccgg actggtaagc atcgcggtta ccagtggaag cagtaatgtg
     2641 ggtatcatgt tgtacaacgg ttgtccgttc atcggacaag gcggcacgtg cgtggattat
     2701 tctcaaagca cttccggcaa ccagtttctg tgtgtgaacg tcgttgccgg caacacctac
     2761 tacctccttg tcgacc
//
LOCUS       DAOTON010000344         2765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28532_length_2765_cov_3.260886, whole genome shotgun sequence.
ACCESSION   DAOTON010000344 DAOTON010000000
VERSION     DAOTON010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28532_length_2765_cov_3.260886"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="P5292_07460"
     CDS             <1..1317
                     /locus_tag="P5292_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRS39003.1"
                     /translation="MIHAKVPLLGHHTSGVRREWYRHDLDEHAKRDPLPRFHSFLLSE
                     GFTVKELDTIRDRSNVRVRADFEKAMKAEDPRPEDLYTYDYAPTPVTEEKGEREPSGK
                     ERTVMVDCALFAVQELMSKHPECLLYGQDVGGRLGGVFREAATLAQKFGDERVFNTPI
                     QEAFIIGSTVGMSAAGLKPIVEVQFADYIWPGLNQLFTEVSRSYYLSNGKWPVSCIIR
                     VPIGAYGSGGPYHSSSVESVLTNIRGIKIAYPSTGADLKGLMKAAYYDPNPVVILEHK
                     GLYWSKIKGTEEARTIEPDEQYIVPFGKARIVQRAEPGKNSSLAIITYGMGVYWARNA
                     SRELADRVEILDLRTLAPLDEAAIYAAARKHGRVLVVTEEPVNNSFAQSIAGRISENC
                     FESLDAPVQVIGSKDLPAIPLNSTLEAEMVPNTQKVAEAIERLLRY"
     gene            1388..1897
                     /locus_tag="P5292_07465"
     CDS             1388..1897
                     /locus_tag="P5292_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRS39004.1"
                     /translation="MESTIYCRMYIQFIVAVRDVNALLPMEELETVRQHFIRFSNKQG
                     VDVLAFSFRPDHLHGLIQVPHDVVLSSFIQSARADISRFLRRRKPEQPAVRWQREIVL
                     LSYSHSQLPAVREFILGQEEYHQRRSFQEELDYILNRTQAEVGEKPFDYILEEGHERM
                     AECFPDAVQ"
     gene            complement(1891..2682)
                     /locus_tag="P5292_07470"
     CDS             complement(1891..2682)
                     /locus_tag="P5292_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRS39005.1"
                     /translation="MCGRYVIVASVEEIEKRFQVTAAQLPFDFQPNFNLGPGSLAPVI
                     TNDKPNELQFFTFGMTPFWAKKRMYLFNARAEGDGNMDNDPDYKGGKGIISKPSFRKP
                     IRSQRCLVIADAFIEGSYAEGLDKPHLVYLYRRPFAFAGIWDVWKNPETEQEVHSFAI
                     ITTTANPLLQKIPHHRSPVILHERDERRWLRAEHLQEITTLLEPYPAEEMNAYPISPK
                     VKSPKATGRELIEPVGERLQPELELRMKTELRLQGMGAGKRDHRH"
BASE COUNT          624 a          759 c          756 g          626 t
ORIGIN      
        1 atgatccacg cgaaagtacc tttgctgggt catcatacct ccggtgtgcg gcgagaatgg
       61 tatcgtcatg atctggacga acacgcgaag cgagatccac tcccgcgttt ccactccttc
      121 ctgctgagtg aaggattcac agtaaaagaa cttgatacga tccgggatcg gtcgaacgta
      181 cgcgtacgcg cggacttcga gaaagcgatg aaggcggagg atccccgtcc cgaggatctc
      241 tatacgtatg actatgcccc tactcctgta accgaagaaa aaggagagcg cgaaccgtcg
      301 ggaaaggaac gcacggtcat ggtggattgt gccctctttg cggtacagga actcatgagc
      361 aaacatccgg agtgcctgtt gtacgggcag gatgtcggcg gtcgcctggg tggagtcttt
      421 cgtgaagccg ctacccttgc gcaaaagttc ggggacgaac gcgtcttcaa cactccgatc
      481 caggaagcat tcatcatcgg cagcaccgtc gggatgagcg cggccggact gaagccgatc
      541 gtggaagtac agtttgccga ttacatctgg cccggactga atcaattgtt caccgaagta
      601 agccgatcct attatctgtc gaacggaaag tggccggtca gttgcatcat ccgcgttcca
      661 atcggcgcat atggcagcgg cggaccgtac cattcctcct cggtggaaag cgtattgacc
      721 aatatccggg gcatcaagat cgcctatccc tccaccggag cggacctgaa gggcctgatg
      781 aaagcggcct attacgatcc gaaccccgtc gtgattcttg aacacaaagg attgtattgg
      841 tccaagatca aaggcaccga agaagctcgg accatcgaac cggacgaaca gtacatcgta
      901 ccattcggaa aagcccgcat cgtacagcgc gcggagccgg gaaaaaattc ctccctcgcg
      961 attatcacct atggcatggg cgtctactgg gcacgaaacg cctcgcgcga gttggctgat
     1021 cgtgtcgaaa ttctggacct tcgcaccctg gcgccgcttg acgaagccgc gatatatgcc
     1081 gccgcgcgca agcacggccg tgtactggtc gttacggaag aaccggtcaa caactccttt
     1141 gcccaatcca ttgccggtcg tatttctgaa aattgttttg aatccctcga tgccccggta
     1201 caggtgatcg gctcgaaaga ccttcccgcg attccactga attccaccct ggaagcggag
     1261 atggtcccga acacgcagaa agtcgccgaa gcgatcgaac ggctcctgcg ttactgatta
     1321 ctccgataac gcactatccc gcttttattg gttgtccgac taccggtcgt gaatgcaact
     1381 tcacgccatg gaatccacca tctattgccg gatgtacatc cagttcattg tcgcggtacg
     1441 ggatgtcaat gcgctgcttc caatggaaga gttggaaacc gtgcgacagc acttcatccg
     1501 tttctccaac aagcagggag ttgacgtgct tgcgttcagt ttccgtccgg accacttaca
     1561 tggattgatc caggtccccc acgatgtcgt actgagttcg ttcattcaat cggctcgtgc
     1621 cgatatcagt cgcttcctgc gacgaaggaa gccggagcaa ccggccgtac gttggcaacg
     1681 cgagatcgta ctcttatcgt acagtcattc gcagttaccg gctgtgcggg aattcatact
     1741 tggtcaggag gaataccatc aacgccggag ttttcaggaa gaactggatt atattctgaa
     1801 ccgcacgcaa gccgaagtcg gcgagaaacc ctttgattat atcctggaag aaggccacga
     1861 gcgtatggcg gagtgctttc cggacgccgt tcaatgacgg tggtcccgct tgccggcgcc
     1921 cattccctga agtcttaatt ccgtcttcat gcgcaattcc agctcgggct gtaagcgttc
     1981 tccgaccggt tcgatcaatt cacggccggt agctttcggg ctcttgacct tgggcgagat
     2041 cggataggca ttcatctctt cggccggata gggctccaac aaagttgtga tctcctgcaa
     2101 gtgctcggcc cgcaaccagc ggcgttcgtc ccgctcgtgc agaatgaccg gactgcgatg
     2161 gtgagggatc ttttgtagca aaggattggc cgttgtcgtg atgatggcga aagagtgcac
     2221 ctcctgttcc gtttccggat tcttccaaac atcccagata ccggcgaaag cgaatggacg
     2281 acggtacaga tataccaaat gcggcttgtc cagtccctct gcatagctgc cctcgatgaa
     2341 agcatcggcg atcaccaggc aacgctgaga acggatcggc ttccggaaag acggcttgct
     2401 gatgataccc ttgccgcctt tgtagtccgg atcattgtcc atgttgccgt caccttccgc
     2461 gcgtgcattg aacaggtaca tgcgcttctt cgcccagaaa ggtgtcatcc cgaaggtgaa
     2521 gaactggagc tcgttgggtt tgtcgttggt gattaccggc gcgaggctgc cgggtcccag
     2581 gttaaagttc ggctgaaaat caaaaggcaa ttgcgcagca gtaacctgaa atcgcttttc
     2641 gatctcctcg acagatgcaa caatgacata gcgaccgcac ataaaagggt tctgggtttc
     2701 ctacttgcgc taaagcttcg gcaggctgaa gggttttggg tttggtggtt cctgctctcg
     2761 tttga
//
LOCUS       DAOTON010000345         2765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28536_length_2765_cov_2.667159, whole genome shotgun sequence.
ACCESSION   DAOTON010000345 DAOTON010000000
VERSION     DAOTON010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28536_length_2765_cov_2.667159"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2355
                     /locus_tag="P5292_07475"
     CDS             <1..2355
                     /locus_tag="P5292_07475"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRS39006.1"
                     /translation="KRNFYWLIVLLTIGLAGAYLYLRYTHPIFQAQAGLKVGIVNNAN
                     AVLNQPGTQMLDLISGSNNAFAGDVELLRSKVMLARVIERMPLEVTYFARGNVLDNEL
                     YEKSPFTVEYEIRDSAYFGVPINITFNSEKEYELNWDYEGLVGSGTYATGVWIETAFA
                     KLRISIVDITSIRKQESKIKKSPFYFILNTTSDLVSAYADDYQVQVVNPIAQTLRVIF
                     KDKNAVKTAEFVNTVLDEFNIYDQERKSEGSNRSLDFINHTITDIDSELKISEISLEM
                     FKRSNKIIDPTENAKDVLSHIKELIDTRVNCQLTLAVLNRMERDIQQSKPIDNLLPLM
                     AGQFDDDVIMRLVERLQELQDKKANLRFVATEENTAIRSIDAEIQLQKQNLTSSIRSA
                     INSQKERILSIDEQIGNFESNFMMLPSKEAELSRLKRFYEVNQKFFQLLLEKKVEFSI
                     TKAGIVSNNIILDRATEPISPLTPNRRLILAGSILLGFIIGFLIIFLKYLFYNEITSL
                     EEINLYTDASLLGIIPKYKSEIPVSQLLVDKNPKSAISESFRSVRTNLQFIQNDPGAK
                     VVAITSTISGEGKTFVAINLAGVIAFSDKKVVILDLDMRKPKIHLGFNVENVRGMSTI
                     LIGKDQPENCINHSNLPNLDFITAGPIPPNPSELILSKRMTDLIAFLKTKYDIVIIDT
                     PPVGIVTDGVQVIRNADYPIYIIRAQYSKRMFIQNLNKLLDENKIQHLSVVLNGVEMS
                     RFRYGYGYGYGYGYGYGYGYGYYDDEMKKNLSRWERFEMQFKKD"
     gene            2365..>2765
                     /gene="rfbC"
                     /locus_tag="P5292_07480"
     CDS             2365..>2765
                     /gene="rfbC"
                     /locus_tag="P5292_07480"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016780253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRS39007.1"
                     /translation="MEFKTTPIADVVMLEPRVFKDERGHFMESFNASLFSAHKITGPF
                     VQDNESVSSKGVIRGLHLQRVPHAQGKLVRVVRGAALDVAVDLRPGSPTFGRHVAVEL
                     SAQNSRMLWIPAGFAHGDEELEDDTVFLYKVT"
BASE COUNT          738 a          700 c          683 g          644 t
ORIGIN      
        1 aagcgaaact tctattggtt aatcgtttta ctgaccatag gtctcgccgg ggcctacctt
       61 tatctccgct acactcaccc gatctttcaa gcacaggctg gactgaaggt tggcattgtc
      121 aataacgcga atgccgtcct gaaccaaccg ggcacacaaa tgctggacct gatatccggg
      181 tctaataacg cttttgccgg tgatgtggaa ttgttgcgat cgaaagtgat gcttgcacgc
      241 gtcatcgaaa ggatgcccct ggaggtgacc tatttcgcgc gtggaaatgt actcgacaat
      301 gagctgtatg agaaatcgcc ctttacggtt gagtacgaga tcagggattc cgcttatttc
      361 ggggttccga tcaacatcac gttcaactcg gagaaggaat atgaactgaa ttgggattac
      421 gaaggccttg ttggaagcgg aacctatgca accggtgtct ggatcgaaac ggcctttgca
      481 aagctgcgga tcagtatcgt cgatattacc tccatccgga agcaggaatc caagatcaag
      541 aagtcgcctt tttacttcat cctgaatacc accagcgatc tggtcagcgc ctatgccgac
      601 gattatcagg tacaggtggt caacccaatc gcccagaccc tacgggtgat cttcaaagat
      661 aagaacgcgg ttaagactgc tgaattcgtc aatacggttc tggatgaatt caacatttac
      721 gatcaggaac gaaagtcgga aggatccaat cggtccctgg attttatcaa tcataccatc
      781 accgacatcg acagcgaatt gaagatttcg gagatctcgc ttgagatgtt caagcgcagt
      841 aacaagatca tcgacccgac cgaaaacgcg aaagatgtcc tcagtcacat caaggaactg
      901 atcgacacac gggtcaattg ccagttgacc ctggccgtcc tcaaccggat ggaacgcgat
      961 atccagcaaa gcaagccgat cgacaacctg cttcccttga tggccggaca atttgacgat
     1021 gatgtcatca tgcgcctggt ggaacgactt caggagttgc aggacaaaaa agccaacctt
     1081 cggttcgtcg cgaccgaaga aaatacggcc atccggagca ttgatgctga gattcaattg
     1141 cagaagcaga accttacttc gtccatccgg agcgcgatca actctcagaa agaacggatc
     1201 ctcagcatcg atgaacagat cggaaatttc gaatcgaact tcatgatgct ccccagcaag
     1261 gaagccgagt tgtcgcgctt gaagcgtttc tatgaggtca atcagaaatt cttccaacta
     1321 ttattggaga aaaaggtcga attctcgata accaaagccg gaatcgtatc caacaacatc
     1381 atcctcgatc gggcgaccga acccatttct cccctcaccc caaacagacg actcatcctt
     1441 gccggcagca tcctgctcgg cttcatcatc ggattcctga tcattttcct caagtatctg
     1501 ttctataatg agatcacctc actggaagag atcaaccttt atacggacgc atccttacta
     1561 ggcattattc caaaatacaa aagcgaaatt ccggtatccc agttgctggt tgacaagaac
     1621 cccaaatcgg cgatctcgga atccttccga tcggttcgga ctaacttaca attcattcag
     1681 aatgatccgg gcgccaaggt ggtagcgatc acctccacga tctccggcga aggaaaaaca
     1741 tttgtggcca ttaaccttgc cggtgtgatc gctttctcgg ataagaaggt ggtgatcctt
     1801 gacctggaca tgcggaagcc caagatccac ctgggcttca atgtggagaa cgtacggggc
     1861 atgagtacca ttctgattgg gaaagaccag ccggaaaact gcatcaatca cagcaacctt
     1921 cccaatcttg acttcattac agccggaccg ataccgccga acccgtcgga gttgatcctg
     1981 agcaagcgga tgacggacct gatcgctttc ctgaaaacga agtacgatat cgtcatcatc
     2041 gatacaccgc cggtgggtat cgtaacggac ggtgtacagg tgatccggaa cgcagactac
     2101 ccgatctaca tcattcgtgc acagtattcc aagcgcatgt tcatccagaa cctcaacaaa
     2161 ttgttggatg aaaacaagat ccagcacttg tcggtagtcc tgaacggtgt tgagatgtca
     2221 cgcttccgat atggctatgg ttacggttac ggctatggtt atggctatgg ctatggctac
     2281 ggctactacg acgatgagat gaagaagaac ctgagccgtt gggagcgttt tgagatgcaa
     2341 ttcaaaaaag actgattgtc gggaatggaa ttcaaaacca cgcccattgc ggacgtagtc
     2401 atgctcgaac cgcgggtatt caaggacgag cgggggcatt ttatggaatc ctttaatgcc
     2461 tccctctttt ctgcgcacaa gattaccgga ccattcgtgc aggacaatga atccgtcagt
     2521 tcgaaaggcg tgattcgcgg gttacacttg caacgcgtac cgcatgctca aggcaaactg
     2581 gtccgggtgg tgcgaggagc cgcgttggat gtggctgttg acctgcggcc gggatcacct
     2641 actttcggac gccatgttgc cgtggagtta tccgctcaga acagtcgaat gctgtggatc
     2701 ccggcaggat ttgcgcatgg cgacgaggaa ctggaagacg ataccgtttt tctgtataag
     2761 gtaac
//
LOCUS       DAOTON010000346         2764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28548_length_2764_cov_3.134736, whole genome shotgun sequence.
ACCESSION   DAOTON010000346 DAOTON010000000
VERSION     DAOTON010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28548_length_2764_cov_3.134736"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="P5292_07485"
     CDS             complement(<1..223)
                     /locus_tag="P5292_07485"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRS39008.1"
                     /translation="MSLTLLILLVTVAVSMIAFSNPGLQDKLMFKPYRIDGNREWYRF
                     VSSGLIHADWMHLLVNMFVLFSFGEIVEFY"
     gene            complement(228..1328)
                     /gene="ychF"
                     /locus_tag="P5292_07490"
     CDS             complement(228..1328)
                     /gene="ychF"
                     /locus_tag="P5292_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRS39009.1"
                     /translation="MGLKCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNLGVI
                     TVPDDRLNKLELLVKPQRIVPTTIEIVDIAGLVKGAHKGEGLGNQFLSNIRECDAIIH
                     VVRCFDDPNVVHVDGSVNPVRDKEVIETELQLKDLESVDKKIQRIEKVAKTGGDKEAL
                     KAVETLKIYKEHLEQGRSARSAPVAPEDKRHIADIHLLTAKPVLYVCNVDEKSVQTGN
                     EYVTRLREAVKDENAELLVIAAAIEAEITQLDSYEDRKAFLDDLGLTESGVSQLIKAA
                     YRLLSLQTYFTAGEKEVRAWTITRGMLAPQAAGVIHTDFEKGFIRAEVIHYEDFVNLG
                     SETACRDAGKLSVEGKEYEVQDGDVMHFRFNV"
     gene            complement(1362..2273)
                     /locus_tag="P5292_07495"
     CDS             complement(1362..2273)
                     /locus_tag="P5292_07495"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HRS39010.1"
                     /translation="MKRILLFTTIFLALLINRSTAQELNCQVSVLTPQIQQTDKTLFE
                     TMQNSIREFMNNRVWTQDKFLNQERIDCSITITISERVSVDEFRGNIQIQARRPVYKT
                     SYNSPLFNHQDNDLSFRYLQDQVLEYDESNISSSTNLTAILAFYAYIILALDYDSFSK
                     DGGTPYFVKAQTIVNNAQAIPERGWKAFESNRNRYWFTENYLNVSFKPLRNCIYQYHR
                     LGMDRLTENIQDGRAAITEALKLLRTTYADRPNSIAMQSFFNAKSDEIVNLYSQAQGD
                     ERNQVVPVLSLVDPANTLKYQNMGQTK"
     gene            complement(2281..2742)
                     /locus_tag="P5292_07500"
     CDS             complement(2281..2742)
                     /locus_tag="P5292_07500"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HRS39011.1"
                     /translation="MVTALEMQAACMDRFQEQDVVVMCAAVADFRPENAAESKIKKDS
                     GELNIALVRNPDILAGMGARKSDRQLLIGFALETTDGLEAAKEKLKKKNLDLVVLNSL
                     QDTGAGFGHDTNQVTLIGPHDAKRLPLLSKSDTAQHLVDYIRDRWQNLHRS"
BASE COUNT          603 a          710 c          734 g          717 t
ORIGIN      
        1 agtagaattc gacaatctca ccgaacgaga acagcacgaa catgttcacc agcaagtgca
       61 tccaatcggc atggatcagt ccgctactga cgaagcggta ccattcacgg ttcccgtcga
      121 tgcggtaggg tttgaacatc agtttatcct gaagcccggg attgctgaat gcgatcatgg
      181 aaaccgcgac cgtcaccaaa aggatcaaca gggtcagact catagcctta tacgttgaaa
      241 cggaagtgca tgacatcgcc gtcctgcact tcatactcct tcccttccac gctcagtttt
      301 ccggcatccc ggcatgcggt ttccgaaccc aggttgacga aatcctcgta gtggatcact
      361 tcggcgcgaa tgaatccctt ttcgaaatcc gtgtgaatga caccggcagc ttgtggcgcc
      421 agcattcccc gggtaatggt ccatgcccgg acttccttct cccctgcggt aaaatacgtt
      481 tgcaaactaa ggagtcggta agccgccttg atcaactgac tcacacccga ttcggttaaa
      541 ccaagatcat cgaggaatgc tttccggtct tcgtaactgt cgagttgggt gatctcggct
      601 tcaatggcag cggcaatgac gagtaattca gcgttttcgt ccttcaccgc ttcgcgtaaa
      661 cgcgttacat attcattgcc ggtctggacc gatttttcat cgacgttaca aacgtagagc
      721 accggtttgg cggtcagcag gtggatatcg gcaatgtgcc gtttgtcctc cggggccacc
      781 ggtgcactgc gcgccgaacg tccctgttcg aggtgttcct tgtagatctt aagggtttcc
      841 acggctttca acgcctcctt atctcccccg gtctttgcga ccttttcaat acgttggatc
      901 tttttgtcga ccgattccag gtccttcaat tgcagttccg tctcgatcac ttctttgtca
      961 cgaaccgggt tgaccgatcc gtcgacgtga accacgttgg ggtcgtcgaa acaacggacc
     1021 acgtgaatga tcgcatcgca ctcccggatg ttactcagaa actgattgcc gagcccttca
     1081 cccttgtgcg cacctttgac cagtccggct atatcgacga tctcgatcgt cgtgggaacg
     1141 atgcgttgcg gtttgacgag caattccagt ttattgaggc gatcatccgg aacggtgata
     1201 acacccagat tcggttcgat cgtgcaaaat ggaaagtttg cagcttgcgc tttggcgttc
     1261 gaaagacaat tgaagagcgt ggatttcccg acgtttggaa gtcccacgat tccgcattta
     1321 agacccataa tcagcagtag taattggtat gtgtggtcga cttacttcgt ctgacccatg
     1381 ttttggtatt tcaacgtatt ggccggatcg accagcgata aaacagggac cacctgattg
     1441 cgttcatcgc cttgtgcctg gctgtagagg tttacgatct cgtcgctttt ggcattgaag
     1501 aacgattgca tcgcaatgga gttcgggcgg tcagcgtagg tggtccgaag cagtttcagg
     1561 gcttcggtaa tggccgcgcg tccgtcctgg atattttccg tcaggcgatc catgccgagc
     1621 cgatgatact gataaatgca gttgcgcaac ggcttgaagg atacgttgag gtaattttcc
     1681 gtgaaccaat agcgattccg gttgctttca aaagctttcc agccgcgttc cggaatcgcc
     1741 tgggcgttgt tcacgatcgt ctgtgctttt acgaagtatg gggtaccacc atccttggaa
     1801 aacgaatcgt aatccagtgc aaggatgatg taagcataaa aggccaggat agccgtgagg
     1861 ttggtgctgg aggagatatt gctttcatcg tattccaaca cctggtcctg aagataccgg
     1921 aagctcaggt cattgtcctg atggttgaac agtggcgagt tgtaggaggt cttatagaca
     1981 gggcgacgcg cctggatctg gatattgccc cgaaactcat cgaccgatac ccgttcggag
     2041 atcgtaatgg taatgctgca gtcgatccgc tcctggttca ggaacttgtc ctgtgtccag
     2101 acccggttat tcatgaattc ccggatcgag ttctgcatgg tctcgaagag ggtcttgtcc
     2161 gtttgctgaa tctgtggcgt caacacagaa acctggcaat tgagttcctg tgcggtcgac
     2221 cgattgatca gaagggccaa gaaaatggtg gtgaacagca gaatgcgttt catgacggag
     2281 tcagcttcgg tggagatttt gccagcggtc gcgtatgtag tcaaccaggt gttgggcggt
     2341 gtcggatttt gacagcaaag gtaatcgctt cgcatcatga gggccaatca gggtgacctg
     2401 gttggtatcg tgaccaaacc cggcgccggt gtcctgaagg ctattcaaaa cgaccaggtc
     2461 gaggtttttt ttcttcaatt tttctttggc ggcttcgaga ccatccgtgg tttcgagggc
     2521 gaatccgatc agcaactgac gatcagattt acgagcgccc attccagcga ggatgtccgg
     2581 attacgaacg agtgcaatgt tcaactcccc ggagtctttc tttattttac tttccgccgc
     2641 attttcggga cggaaatcgg ctaccgccgc gcacatcacc acgacgtcct gctcttggaa
     2701 gcggtccatg caggccgcct gcatttccag ggcggtgacc acgttgaccc gggtatctat
     2761 tccg
//
LOCUS       DAOTON010000347         2754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28701_length_2754_cov_3.427195, whole genome shotgun sequence.
ACCESSION   DAOTON010000347 DAOTON010000000
VERSION     DAOTON010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28701_length_2754_cov_3.427195"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1282
                     /locus_tag="P5292_07505"
     CDS             146..1282
                     /locus_tag="P5292_07505"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRS39012.1"
                     /translation="MVSRLNFRIVWSILISLSLLPESSPAQPTALSAVESRISRFRID
                     HPESSLAFGFLEGEKSRELYFADQTGGQQPDTATVFRLGRLTALWTTTMLVSCSQQGL
                     LSLDAPVSDYLPVNVPSPVYQRLVCQPVKRTEQTPSPDDPRFTPYACLPDPSDKPQPI
                     LLCYLSTHTSGLPDQPFTLERKNGVPDYPGFTVGSLYKFLSIYRIEQPIGFDYVHSDL
                     GMVLLGHVIQRKTGQTLDSLFRQEVAVPLGLTSSRLLTGTEPGEYGLYAAAIGGEASF
                     ADLLRYLRFNLHPAGKIGQVMEYMHTPRVRIREHEEIALGWHCRTFEGNGRRVLYMNG
                     SESGQTVMMVLEESGKSGFVLAGTGNGLAELGDQLLSLLYSKPE"
     gene            1279..2049
                     /locus_tag="P5292_07510"
     CDS             1279..2049
                     /locus_tag="P5292_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRS39013.1"
                     /translation="MTPVNLVLSGGGARGIAHLGVIKALMESGLVINAISGVSAGAIC
                     GAFHAAGYSPEDTLKIVKESKLLYLLRPSFNDGLFKSDKIGEVLRKYLAKSTFESLNI
                     PLYVSASDISEGHTDFFSQGDLIVPLLASSALPVIFQPVTLDRDQLVDGGILNNLPVE
                     PFLEDDYTLVGVHVNPWTNGVKAESAYSVMERSIQLSIYGTVLTRKHHCDLFLEPEEL
                     KNYSMYDLDHADKLFEIGYLFAKERLNRFMASGHHCAR"
     gene            complement(2058..2582)
                     /locus_tag="P5292_07515"
     CDS             complement(2058..2582)
                     /locus_tag="P5292_07515"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HRS39014.1"
                     /translation="MSLLSSGLFWGIVIVFLGLSIILREIFDVHFPFLRVLFGAVLIW
                     WGVRMIAGGFQKNETVAFSETDSGWDDGKKEYSVVFGRGNFDLFKAPPITSDRKLEVN
                     VVFGNGKLILNDSIPTIVKTNTVFGTTRTPNGTTAAFGETAFRTSTYKEGEPALKIET
                     NTVFGQLEIETKRW"
BASE COUNT          591 a          714 c          763 g          686 t
ORIGIN      
        1 gaccgtgtca ggatcgggaa ggagagtctg gagttcgcgt ggaaattgat caacgggctg
       61 tttgattcct ggaagggtac caacgtttcg gaacaggagc aactgctgca ttttcaccgg
      121 gtgttttttg gaaaggatga gaacgatggt gtctagattg aatttccgta ttgtctggtc
      181 gatcctgatc tcgctctcgt tgctgccgga gtcaagcccg gcacaaccga ctgccttatc
      241 tgcggtcgaa tcgcgtatca gccggttccg aatcgaccat ccggagtcca gccttgcttt
      301 cggattcctg gaaggggaga agagccgtga actttacttt gccgatcaga cgggcggaca
      361 acaaccggac actgcaaccg ttttccgtct gggaaggttg acagctctct ggaccacgac
      421 catgcttgtc tcgtgttccc agcagggatt gctttcgctg gatgccccgg tcagcgacta
      481 tctgccggtg aatgttccat ctcctgttta ccagcgactc gtctgccaac cggttaaacg
      541 gacggagcaa actccttcgc cggatgatcc gcgcttcact ccgtatgcgt gcctgccaga
      601 tccttccgat aagccccagc ccatcctgct ctgttacctt tcgacgcata catccggctt
      661 gccggatcaa ccgttcacgc tggagcggaa gaacggtgtt cccgattacc ccggcttcac
      721 cgtcgggtcg ctttacaagt tcctatcgat ctaccgtatt gaacaaccca tcggcttcga
      781 ctatgtccac tcggatctgg gtatggtctt gctggggcat gtcatacaac gaaagacggg
      841 tcagacactc gattccttgt ttcggcagga ggtcgccgtc ccgcttggac tcacttccag
      901 tcgcctgcta acggggactg aaccgggtga atacggattg tatgcggctg ccatcggcgg
      961 agaagcgtcc tttgccgatc ttctgcgtta cctgcgattc aacctgcatc cagcaggcaa
     1021 gatcgggcag gtgatggagt atatgcacac cccccgggtc cgtattcgcg aacacgagga
     1081 gatcgcgttg ggttggcatt gccggacgtt tgaagggaac ggtcgtcgtg tcttgtatat
     1141 gaacgggtcg gagtcgggac aaaccgtgat gatggtgctg gaagaatccg gcaagagtgg
     1201 attcgtgctg gccggtaccg gtaacggtct ggccgaattg ggagatcaac tactttcgtt
     1261 gctttattcg aaacccgaat gacacctgtc aatcttgtac tttccggcgg tggcgcgcgc
     1321 ggcattgccc acctgggtgt gatcaaggcg ctgatggaaa gcggtctggt catcaacgcg
     1381 atctccggcg tgagcgccgg ggccatctgc ggcgctttcc atgcggcggg ttattcgccc
     1441 gaggatacgc tgaagatcgt caaggaaagc aagctcttgt acttgctccg tccgtctttc
     1501 aacgacggct tattcaagag tgataagatc ggtgaagtgc tccggaaata tctggctaaa
     1561 agcacctttg aaagcctgaa tatcccgttg tatgtcagcg cttccgatat ttccgaaggt
     1621 cataccgatt ttttcagcca gggcgatctc atcgttccgt tactggcctc ctcggctttg
     1681 ccggtcatct ttcaaccggt aacacttgac cgggaccaat tggtcgacgg gggtattctt
     1741 aataaccttc cggtggaacc cttcctggaa gacgactaca ccctggtcgg cgttcacgta
     1801 aatccctgga caaatggtgt caaagcggaa tcggcgtaca gcgtgatgga acggagcatt
     1861 cagctttcta tctacggcac ggtcctgacc cgtaagcatc attgtgatct tttcctggaa
     1921 ccggaagaac tgaaaaacta tagcatgtac gatctcgatc acgcggataa attattcgag
     1981 atcggttacc tgtttgcaaa ggagcggttg aaccgcttta tggcctccgg tcaccattgt
     2041 gcccgctagg gggtgcttta ccagcgtttt gtttcgattt cgagttgacc gaagaccgta
     2101 ttggtctcga ttttcaatgc gggttcacct tccttgtaag tagaggtgcg aaaggctgtt
     2161 tccccgaacg cggcggtcgt accgttcggt gtgcgcgtcg tgccgaatac cgtattcgtt
     2221 ttgacaatgg tcggaatgga atcgttgagg atcagcttgc cattgccgaa aacgacattg
     2281 acttccagct tccggtcgga cgtgatcggt ggcgctttga agaggtcgaa atttcccctg
     2341 ccgaacacca cagaatactc cttcttcccg tcatcccatc cggaatccgt ttcggagaac
     2401 gcgacggttt catttttctg aaagcctccg gcaatcatac gaacgcccca ccagatgagg
     2461 acagctccga ataatacccg caagaagggg aaatgtacgt cgaagatttc ccgaaggatg
     2521 atggaaaggc cgaggaatac gatgacgatt ccccaaaaca aaccggagga aaggaggctc
     2581 atggatcgtt ggtttgacat gtggatggat gatgtcgaat gaaatcagtt cccggatgct
     2641 tttttccgga agtacttacg aaacaggaaa ttctgctgca gggctttcat gttttcgttg
     2701 aagctggccg tgctggaatc gatattcgca atgctgcggc gcaaatggtc ggct
//
LOCUS       DAOTON010000348         2751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28752_length_2751_cov_3.043027, whole genome shotgun sequence.
ACCESSION   DAOTON010000348 DAOTON010000000
VERSION     DAOTON010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28752_length_2751_cov_3.043027"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="P5292_07520"
     CDS             <1..615
                     /locus_tag="P5292_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRS39015.1"
                     /translation="DLEASRKHLLEVQDRSSQATAEFFQPKSGETWWDACAGSGGKSL
                     LLLDQEPGLHLTVSDRRPSILKNLKERFGLAKLKPDELVVCDLTQPETDWPEQMFDVI
                     LADVPCSGSGTWTRKPEGLFTEVDEAFFQRFRSLQQKIVKNLSSRIKPGGRLIYLTCS
                     VFSAENEEMLSWVEQETNLKLQQYRYFEGAAYRADTLFGASFLN"
     gene            715..>2751
                     /locus_tag="P5292_07525"
     CDS             715..>2751
                     /locus_tag="P5292_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39016.1"
                     /translation="MYLRILFILTGLLLTQVVVSGQNQANSESELRKRADKLFQDQEY
                     LTAVGPYSQLLSLQPNDATLNYRYGTALLLSGKDKRNAVSYLEKAMNDPAQPMEGWVY
                     LGRSYMFLEKYEDAKSCFNRFNTRATDAQRKKFNSEEWLRSAGAASELSRQRRMVSVT
                     AQKEVLRSAAYSQIDYTNAAGRILPVPEKFQTTADKDKQAAPVMYLSRDGQTIYYASY
                     GKGSVGGKDLYLIRKMTNGEWAQPEHLGSVLNSVYNEDYPFLDRDGKTLYFASQGHNS
                     IGGYDIFKSVFNFNTGQWSAPENLGIPVNTPDDDLLYVPGSDGVTAVYTTADFTDNTH
                     TEIRTIHLDDQRNPFATIRGTYYSIDQPMRRDARVIVLRAADRSVVTTVRTDPRTGGY
                     ELVLPGGESYWLVIDGGGYVPHTESFDLPAGLEKAALRQEVRMNRNKQEELMTVTNFF
                     TPMPSAIGADVIAVNDRPSSTETSATTPSSETVQISVGNETLIVSKPVSQPVTSPEQA
                     GEVPVTADQAADEDTSLADNYNPLLDREMTQDEIRQRQEEQLRSEEVVADEKIPAEQL
                     HVPVDEHELARNAVEDGVALQREADSLQDRSRELYASAANREEEAVRLLEEAQSIKED
                     TVRAANLYLQSDDMKQMALAEKQQARELDGLATERRAEAKQALTDAKALISSTNT"
BASE COUNT          709 a          715 c          737 g          590 t
ORIGIN      
        1 gaccttgagg caagcagaaa gcacctgctc gaagtgcagg atcgtagcag tcaggctacc
       61 gctgaatttt tccagcccaa gtcgggtgaa acctggtggg atgcctgcgc aggttccggt
      121 ggtaaatccc tgttattgct ggaccaggaa ccgggcttgc acctcacggt ttctgaccgg
      181 cgcccgtcga tcctgaaaaa cctgaaagag cggtttgggc tcgcaaaatt gaaaccggac
      241 gagttggtgg tctgtgacct gacccaaccg gagactgact ggccggagca gatgttcgat
      301 gttattttag ccgatgttcc ctgttcagga tcaggaactt ggacaagaaa accggaagga
      361 ctttttaccg aagtggatga agccttcttt cagcggttca gaagcctgca gcagaaaatc
      421 gtcaagaatc tttcatccag gattaagccc ggcggccgcc tcatttatct gacatgttca
      481 gttttttctg ctgaaaatga agaaatgctt tcctgggtag agcaggaaac caacctgaaa
      541 ctacagcaat accgctattt cgaaggtgcc gcttacaggg ccgatacact gtttggtgct
      601 tcttttctta attaagcccc aatagcttgt aaatcaatca aataatgcca ctgcaggcag
      661 gaactattcg ggcttggatg aagtatacaa tctaatccca caccagggag tattatgtac
      721 ctacgaatcc tattcatcct gacgggcctg ttgctgacac aggttgtagt atcaggacaa
      781 aatcaggcta attctgaatc cgagctgcgt aagcgggcag ataagttatt tcaggatcag
      841 gaatatctga ccgctgtcgg gccttattcc cagttgctca gcctgcagcc gaatgatgcc
      901 accctgaatt atcgttatgg cacggccctg ttgctttccg gaaaagacaa acggaacgcg
      961 gtaagctacc tggaaaaagc catgaacgat ccggcacaac cgatggaggg ctgggtgtac
     1021 ctcggacgat cttacatgtt tctggaaaag tacgaggatg ctaaaagttg tttcaaccgg
     1081 tttaataccc gggcaacgga cgcgcagcgc aagaagttca attcggaaga atggcttcgt
     1141 tccgcgggtg ccgcatcgga gttatcccgt caacgcagga tggtcagcgt gaccgcgcag
     1201 aaggaagttt tacgttctgc agcttactct caaatcgact atacgaatgc tgccggtagg
     1261 atcctgccgg tgccggaaaa gttccagacg acagccgaca aagacaaaca ggctgctccg
     1321 gtcatgtatc tgtcgcgcga cggacagacg atctactacg ccagctatgg gaaagggagt
     1381 gtgggaggaa aggacctcta tctcattcgt aagatgacga atggggaatg ggcacaaccc
     1441 gaacacctgg ggtccgtgct aaactccgtc tacaacgaag attatccgtt ccttgaccgg
     1501 gatggcaaaa cgctctactt cgcgagccag ggtcacaatt cgatcggtgg ctacgatatt
     1561 ttcaaatccg ttttcaattt caataccggc caatggtcgg cacctgaaaa cctgggaata
     1621 ccggtgaaca ctccggacga tgatctgctg tatgtgcccg gctccgatgg tgtaaccgcc
     1681 gtatacacca ccgccgactt tacagataac acgcacaccg aaatccgtac catccacctc
     1741 gatgaccagc ggaatccttt cgctaccatt cgcggaacgt attattcgat tgaccagccc
     1801 atgcgtcgcg atgctcgggt cattgtgctg cgtgcagccg atcgttctgt tgtgacaact
     1861 gttcgaaccg atccgaggac tggtggatat gaattggtgt tgccgggcgg tgagtcgtac
     1921 tggcttgtca tcgatggcgg cggctatgtt ccgcataccg aatcattcga cctgcctgca
     1981 ggtctcgaaa aagccgcgct ccgacaggaa gtgcgcatga accgcaacaa gcaggaggaa
     2041 ttgatgacgg tcaccaattt cttcacgccc atgccatctg ccattggtgc tgatgtgatt
     2101 gcagtgaacg accgtcctag ttctaccgaa acatctgcta caactccgtc atcggagaca
     2161 gttcaaatct cggtcggaaa cgaaacgctg attgttagca aaccggtcag tcagccggta
     2221 acatcccctg aacaagccgg tgaagttccg gtaacagccg accaggctgc agatgaggac
     2281 acctcactcg cggacaacta caatccgctg ttggaccggg agatgaccca ggacgaaatc
     2341 cgtcaacgcc aggaagaaca gcttcgctcg gaagaagtgg tcgcggatga aaagatcccc
     2401 gcagagcaac tccatgtgcc cgtcgatgaa cacgaacttg cacgcaacgc cgtggaggat
     2461 ggtgtcgccc tgcaacgcga agcggacagt ctgcaggatc gcagccggga attgtatgcg
     2521 tcggctgcaa accgcgaaga ggaagcagta cgcttactcg aagaagcgca gtccatcaaa
     2581 gaggataccg ttcgcgcagc caacctctac ctgcaatcgg atgatatgaa gcagatggcc
     2641 cttgcggaaa aacagcaggc cagggaattg gacgggcttg caaccgagcg tcgcgctgaa
     2701 gcaaagcagg cattgaccga cgcgaaagcc ttgatcagtt cgacgaatac g
//
LOCUS       DAOTON010000349         2750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28770_length_2750_cov_2.475325, whole genome shotgun sequence.
ACCESSION   DAOTON010000349 DAOTON010000000
VERSION     DAOTON010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28770_length_2750_cov_2.475325"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="P5292_07530"
     CDS             <1..685
                     /locus_tag="P5292_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRS39017.1"
                     /translation="WADESAWGFVRNQLAVHANVEVVNLPAHGVDLSDGKSIGMKDYE
                     RAVVDAINRHPGKVTLVGHSMAGMIISRVAETMPEKVDKLIYVSAFLPKNGESLNGIV
                     NRFLNGQPLDIFEFNSDYTLIGVKKDVLPTVVCEDCPAYMKDVLVKYHRPDPVKAFND
                     TVMLGANFAKVPKYYLSTKNDKAVPYALQQQMIRENGTVRKVYELETSHLPFVVRPEE
                     FVNIIRGIQ"
     gene            682..1350
                     /locus_tag="P5292_07535"
     CDS             682..1350
                     /locus_tag="P5292_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39018.1"
                     /translation="MIARILSRACLLLVLLATAGKAGAQLPVEAFGGDKKATFDALFF
                     HFFRNANDQPSRFLFFSRTRASVDYKITQTTNLPSFGLTEAFSYNAPKLKGVAPVAVV
                     QLLNRGVYPKAGVQYVLLRKEVTLFTWLVSELDNEPVVDHFLLVRYTPKITERYQLFL
                     QAESISAFPTQANENRSFTQRFRLGLMRSAFQFGAGIDLTEVGRDEYTGTQNTGLFLR
                     YDFR"
     gene            1438..2106
                     /locus_tag="P5292_07540"
     CDS             1438..2106
                     /locus_tag="P5292_07540"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HRS39019.1"
                     /translation="MYNFSYFKEQDRQRLLHFMEEHPFAFLTGSTLTGKQVATQLPVL
                     FEERDGELYLQGHLMRNTDHHKAFKENPQALLVFTGPSAYVSASWYSNPQIGSTWNYM
                     SIHIGGTIEFMSDDGLIEFMRKFTLKFEGGNTSSKTIYDNLPDSFLHKMMPAIVGFEL
                     KAERFENVFKLSQNRDEQSYRNIIDRLEERGGESALIAAEMRKRQEELFPPGVEWDGS
                     RFDS"
     gene            complement(2212..2643)
                     /locus_tag="P5292_07545"
     CDS             complement(2212..2643)
                     /locus_tag="P5292_07545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRS39020.1"
                     /translation="MRMRFSSLAIDDAGSMSNSIRGFLFFPPLKGTLTNRFDAVRRHY
                     GIDIVSNPNEPIKATLDGTVVIANFTSETGWVIGIQHSNNLFSFYKHNSALLKKVGDY
                     VKAGDAIAIIGNSGELSSGPHLHFELWFNGTALDPVKYVAL"
     gene            2636..>2750
                     /locus_tag="P5292_07550"
     CDS             2636..>2750
                     /locus_tag="P5292_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease"
                     /protein_id="HRS39021.1"
                     /translation="MRMDDEQESGNVEDRRGMGMPMRSGSVGIGTIILALAA"
BASE COUNT          628 a          752 c          784 g          586 t
ORIGIN      
        1 ctgggccgac gaaagcgcct ggggattcgt gcgcaaccaa ctcgccgtac atgccaatgt
       61 ggaagtcgtt aacctgcccg cgcacggtgt tgacctgagc gatggcaaga gcatcggcat
      121 gaaagactac gaacgcgccg ttgtagatgc tatcaatcgc catcccggaa aagtcacgct
      181 cgtcggccat tcgatggcgg gcatgattat ctcccgcgta gcggaaacaa tgcccgagaa
      241 ggtcgataag ctgatctacg tatccgcctt cctgccaaag aacggtgaga gcctcaacgg
      301 gatcgtgaac cggttcctca acggacaacc cctcgacatt ttcgagttca acagcgatta
      361 taccctgatc ggtgtgaaga aggacgtgtt gccgaccgtc gtttgcgagg attgtccggc
      421 ctacatgaaa gatgtgctcg tgaaatacca ccgtccggac ccggtgaagg cgttcaacga
      481 tacggtgatg ctcggtgcca attttgccaa agtaccgaag tactatctca gcacgaaaaa
      541 cgacaaggcc gtaccctacg cgctgcaaca gcagatgatc cgcgagaacg gaaccgtccg
      601 caaggtatac gaactggaaa cgtcccacct gccgtttgtc gtccgacccg aagagttcgt
      661 caacatcatc cgcggaatcc aatgatcgca cggatcctct cccgcgcctg tctgctcctg
      721 gtcttgctcg caactgcggg caaggccgga gcacagttgc cggtcgaagc gttcggcggc
      781 gacaagaagg cgaccttcga tgccttgttc ttccattttt ttcgaaatgc caacgaccag
      841 ccctcgcgtt ttctcttctt cagccggacg cgcgcttcgg tcgactacaa aatcacccaa
      901 acaacgaacc tgccttcgtt cggactgacc gaagcgttca gctacaacgc cccgaagctg
      961 aaaggcgtag ctcccgtggc ggtggtgcag ttgttgaacc gcggcgtgta tcccaaagcc
     1021 ggtgtacaat acgtgttgtt gcgaaaggag gtcaccctct tcacctggct ggtgagcgaa
     1081 ctggacaacg agcccgtggt ggatcacttc ctgctcgttc gctatactcc gaagatcacg
     1141 gagcgttacc aactctttct ccaggccgaa tcgatcagcg cttttccgac gcaagcgaat
     1201 gagaaccgct ccttcacaca acgctttcgg ctcgggctca tgcgttcggc ctttcagttc
     1261 ggagccggca tcgatttgac cgaagtcggt cgcgacgaat ataccggtac gcagaacacc
     1321 ggactgttcc tgcgctacga ttttcggtga cctctgtgca caaccggttc ctaagtatcg
     1381 aaggaagctt agtatcttgt gcttcgggcg tatcgatacg ttcgtttatc cggcgttatg
     1441 tacaactttt cttatttcaa agagcaggac cggcagcgac tcctgcattt catggaggag
     1501 catcccttcg cgttcctgac cggaagcacc ttaacgggca aacaggtggc gacccagctg
     1561 cctgtcttgt tcgaagaacg tgacggtgaa ttatacctgc agggccattt gatgcggaac
     1621 accgatcacc acaaggcgtt taaagaaaat ccccaggccc tgctcgtctt caccggcccg
     1681 agcgcttacg tgagcgcttc ctggtacagc aatccgcaga tcggttcgac ctggaactac
     1741 atgagcattc acatcggtgg gacgatcgaa ttcatgtcgg acgacgggct gatcgagttc
     1801 atgcggaaat tcaccctgaa gtttgaagga ggaaacacca gctccaagac catctacgat
     1861 aacctgccgg attcctttct ccataagatg atgccggcga ttgtcggatt tgagctgaag
     1921 gcggagcggt tcgaaaacgt tttcaagttg agccagaacc gcgacgagca gagttatcgg
     1981 aacatcatcg accggttgga ggagcgcgga ggtgaaagcg ccctcatcgc agcagagatg
     2041 agaaagcgac aggaggagtt gtttccgccg ggggtggagt gggatggatc tagattcgat
     2101 tcgtaaagta tactgtttta cgaaacggca ggacacgcca agcttcgatt cgagattcct
     2161 gacaagcccg cggaatagtt tccgagcgag aagttgattt agttgaatcg cttagagcgc
     2221 cacatacttc accggatcca gcgcggtgcc gttgaaccag agctcgaagt ggaggtgcgg
     2281 accggagctg agttcgccgg agttgccgat gatggcgatg gcgtcgccgg ctttgacgta
     2341 gtcgccgact tttttcaaca aggcgctgtt gtgcttgtaa aaactgaaaa ggttgttgct
     2401 gtgctggatg ccgatcaccc aaccggtctc ggaggtgaag ttcgcgatca cgaccgtgcc
     2461 gtcgagcgtc gctttgatgg gttcgttcgg gttggagaca atgtcgatgc cgtagtgccg
     2521 gcgcaccgcg tcgaaacgat tggtgagggt tcctttcagc ggagggaaaa acaggaagcc
     2581 tcggatgctg ttggacatcg aaccggcgtc atcgatggca agcgaggaga atctcatgcg
     2641 catggatgac gaacaggaaa gcggcaacgt cgaggaccgg cgcggcatgg gcatgcccat
     2701 gcgctccggc agcgtcggca tcggcacaat catcctggcg ctggcggcca
//
LOCUS       DAOTON010000350         2747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28805_length_2747_cov_3.316865, whole genome shotgun sequence.
ACCESSION   DAOTON010000350 DAOTON010000000
VERSION     DAOTON010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28805_length_2747_cov_3.316865"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..520
                     /locus_tag="P5292_07555"
     CDS             119..520
                     /locus_tag="P5292_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39022.1"
                     /translation="MIGWMAISSCRKENDSQIPNVPVNLTLYLSLPEYSALNSVGNSI
                     TINGGYKGIIVYRKSIDDFAAYDRACPYDPTASGSILNVDSSLVQVKDDKCGSRFSLF
                     DGSITNGPASRPLRAYGTEYDAAAQSLYIHN"
     gene            854..2221
                     /locus_tag="P5292_07560"
     CDS             854..2221
                     /locus_tag="P5292_07560"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRS39023.1"
                     /translation="MKLTRRAVQHLFLRAGFGALPAELDRWEGKGVEDLVDHLFNESE
                     QIEDLNLIPDPLKGRAEVSNFRVLAMILRSGPEMQNLNFHWLYRMVESRACLREKATL
                     FWHGHFATSAPFSYLMQGQNNFLRRHALGSFRDLLHGIAQDPAMIIYLNNQENHKDHP
                     NENFAREVMELFTLGIGNYTEHDIREAARAFTGWKVNRKGKFEILDTDHDTGTKVVLG
                     KTGPLDGTEVLDLLLAHPALPDFLSGKLLRFYFHPNPSDERIRELAGVLQEHDLHIGK
                     TLRHLFTRDWFYDASVMGSMIAAPVPLLVRMIRYFGIEFERGETLLRLQQNLGQTLFF
                     PPNVAGWKGHRDWIDSASLLLRLSLADRFLRERALTARGRPEFEETSQKKLQEEAIAR
                     ITARFEIPEKAYGAQTPDTWIDALSRDLLTTDLSSSTRKALPTPATLKDACIAVCSLP
                     EFQLV"
     gene            2224..>2747
                     /locus_tag="P5292_07565"
     CDS             2224..>2747
                     /locus_tag="P5292_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal"
                     /protein_id="HRS39024.1"
                     /translation="MLRRDFLRTTGLASTALFLPAFLRGSVTQSFSGRRVIVIQLTGG
                     NDGLNCIVPFRDDHYYSARPGIALRNNELLRLNDDAAFNINLRGLADLFHKGEASILS
                     SVGYPNPNRSHFRSMDIWHSASDADRYVQTGWIGRYLDALKSSNATPAHLALELDESL
                     SLALKGEQRKGMATP"
BASE COUNT          632 a          829 c          639 g          647 t
ORIGIN      
        1 aatacaatat tcgtttcatc gttgtatttt tgatcaaagt tagacccaac accctgacct
       61 ccttccattg cccggacgca cttttcaacc tggacctatc aactacctcc tgctcctgat
      121 gatcgggtgg atggccattt cctcctgtag gaaggaaaac gattcccaaa tccccaatgt
      181 tccggtcaat ttgaccctct atctgagcct tcccgaatac agcgccctca acagcgtcgg
      241 caactctatc accatcaacg gcggttataa gggaattatt gtttaccgaa aaagcatcga
      301 cgatttcgcc gcctacgacc gtgcatgtcc ttatgacccc acggccagcg gctccatcct
      361 gaatgtggac tccagcctgg tacaggtaaa agacgacaag tgcggtagcc gcttcagcct
      421 gttcgacgga tccatcacca atggtccggc atcacggccg ctgcgtgcgt atgggacaga
      481 atacgatgcg gcggcgcaga gtttgtatat tcataattga gtgattagtg aacagtgaac
      541 agtgaacagt gaacagtgaa cagtaaatag tgaatagtga atagcgaata gtaaatcctg
      601 tccgccgtag cttttacgaa ggcgggagcg aatagttacc agttattagt ttctagctat
      661 tattagttac tagtttctag tttctagttt ctagtttcta gttgcccttt ctgacacctt
      721 gtaaccagcg ccctccttca tgctttcttg ccatttaccc tatcgaactt ttagtcaatt
      781 ttatggcatg tgttttgttt tcctgattca cccccattaa tttctaattt tctttccact
      841 taatcagtcg tccatgaaac ttacgcgtcg ggctgtccaa catctttttc ttcgtgccgg
      901 ttttggcgcg ctgccggcag agttggatcg ctgggaagga aagggagttg aagacctagt
      961 ggaccatctt tttaacgagt cggaacagat cgaagacctg aacctcatcc ccgatccctt
     1021 gaaaggtcgg gccgaggttt ccaacttccg cgtattagcc atgatcctcc gttccggacc
     1081 ggagatgcag aacctcaact ttcactggct ttaccggatg gtcgaatcgc gggcttgcct
     1141 gcgcgaaaag gcgaccctct tctggcacgg acatttcgcc acctccgccc cattctccta
     1201 cctgatgcag ggtcagaaca attttctgcg acgccatgcg ctcggttcct tccgggacct
     1261 gttgcacggc attgcacaag accccgcaat gatcatttat ctcaacaacc aggaaaatca
     1321 caaagaccat ccgaacgaga acttcgcccg cgaagtgatg gagttgttca ccctgggcat
     1381 cggtaactat actgagcacg atatccggga ggccgcacgc gccttcaccg gctggaaagt
     1441 gaatcgcaag ggaaaattcg aaatcctcga caccgatcac gataccggaa ccaaagtagt
     1501 cctggggaaa accggcccgc tcgacggtac cgaggtgctc gatctgctgc ttgcacaccc
     1561 tgccctaccc gacttccttt ccggcaaact gcttcgattc tacttccatc ccaacccatc
     1621 cgatgagcgc attcgggaac tagccggtgt gttacaagaa catgatcttc atatcggcaa
     1681 aaccctccgc cacctgttca cacgggattg gttttatgac gcctcggtga tgggatccat
     1741 gatcgcggct ccggtaccgc tgctggttcg catgatccgc tacttcggta tcgagttcga
     1801 acggggcgaa acacttctta ggctacaaca aaacctcggt cagacacttt tcttccctcc
     1861 caatgtggca ggatggaaag gacaccgcga ctggatcgac agcgcctctc tactcttgcg
     1921 gctcagcctc gccgatcgct ttttacgcga acgcgcctta acggctcgcg gacggccgga
     1981 gtttgaagaa acttcacaaa agaaacttca ggaagaggct atcgcccgca tcaccgcccg
     2041 attcgaaatt cctgaaaaag cctatggcgc tcaaactccg gatacctgga tcgacgcatt
     2101 gtcgcgggac ctgctgacca cggacctttc ctccagcaca cgaaaggcac tcccgacacc
     2161 agccaccctg aaggatgcct gtatagccgt ttgcagcctg cctgaattcc aactcgtctg
     2221 accatgcttc gtcgcgactt cctccgaaca accggccttg cctccacagc gctgtttctg
     2281 cccgcctttc ttcgcggttc cgtcacacaa tcgttcagcg gtcgcagggt gatcgtgatc
     2341 cagctcaccg gaggcaacga cgggctcaac tgcatcgttc ccttccgcga cgaccactac
     2401 tactccgccc ggccgggtat cgccctccgc aataacgaat tgctccgact aaacgacgat
     2461 gccgctttca acatcaacct gcgcgggttg gccgatctgt ttcacaaagg cgaagcaagc
     2521 atcctgagca gcgtcggtta ccccaacccg aaccgctccc acttccgctc catggatatc
     2581 tggcatagcg ccagcgatgc cgaccgctac gttcaaaccg gatggatcgg ccgttacctg
     2641 gacgcgctga aatcctccaa tgcaacgccc gcacacctgg ccctggaact ggacgaaagt
     2701 ctcagcctgg cattgaaggg cgaacagcgt aagggcatgg ccacacc
//
LOCUS       DAOTON010000351         2743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28863_length_2743_cov_3.572545, whole genome shotgun sequence.
ACCESSION   DAOTON010000351 DAOTON010000000
VERSION     DAOTON010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28863_length_2743_cov_3.572545"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="P5292_07570"
     CDS             <1..1077
                     /locus_tag="P5292_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39025.1"
                     /translation="TLFKNILNATTYFEIGTGQERRQEFYYLEVPAGQGTYAWLGDLN
                     GNGVKDLDEFAISNFPDQAKYVRVSVNTNDYITVRSNQFSEVLNINPAAARTNYSGKP
                     RFIEHFSNQFLLRLDKRTRGEELLSSLNPFSRRLDDSLLVTMNSSYRNMFFINRNDPV
                     WGMDYTWQQNRNKSFLSNGFESRTLTTQSINGRWNMSQHFQLSGTAEQELRENNSEYF
                     KTRNWRILSNALEPRISYQRGVDFRTTLSYRYTEKRNLYGDTGEKAVLHKLGIDSRLN
                     SVNAGTFNARFNVIRIDYNAPDNSQLAYELLEGLKDGDNYTWGLSLLRSLGKSMQLTL
                     NYDGRKSTGSPVVHTGGVQFRAFF"
     gene            complement(1134..2063)
                     /locus_tag="P5292_07575"
     CDS             complement(1134..2063)
                     /locus_tag="P5292_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39026.1"
                     /translation="MDSQDANKTDYQEQGPKKGIVIIVITILLGTNGLLLWQFFEKKN
                     SLDAANQTILSTTAEKDALQNQLNQVRAEYEKIKQDNAGLQSQLAERDEEIKSKMAEI
                     QRLIALGGPAQIARAKAELAKLKEMNQVYTVQIDSLNKVNAQLQAENMNLNSNLLEER
                     SRSENLSSENNRLSTKVAAGSVLKALNVTTEGVRFRSTGKEVLTNKAKQVQKIRTKFT
                     LAENKVIDRGPVDLYIRVIGPDGAVMSASTETFKANGNDLNFTLKQTVEYTNSDTPVE
                     VSWAKGSEFPKGKYTVEIYHAGLVVGKSGIDLK"
     gene            complement(2086..2352)
                     /locus_tag="P5292_07580"
     CDS             complement(2086..2352)
                     /locus_tag="P5292_07580"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HRS39027.1"
                     /translation="MQLPPFVQLIYIFHSSGMVALGKLKNPATDKIERDLGQAKQSIE
                     MLEMLKDKTSGRLTDEEQRMLDGVLTELRLNYVDEVNKDKISAN"
     gene            2473..>2743
                     /locus_tag="P5292_07585"
     CDS             2473..>2743
                     /locus_tag="P5292_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HRS39028.1"
                     /translation="MAASRKKYSLRNRVALLRSGERYFEVLLRMIADARQEFHLQTYI
                     LEPDTTGRLVLEALIAAAARGVAVYIQLDAYGSGKLDETMLSDLRS"
BASE COUNT          607 a          750 c          700 g          686 t
ORIGIN      
        1 acactcttca agaacatcct caacgccacc acctatttcg agatcggtac aggtcaggaa
       61 cgccggcagg agttctacta ccttgaagta ccggccgggc aaggcacgta tgcctggctg
      121 ggcgacctga acggaaacgg cgtaaaagac ctggatgaat tcgccatctc caattttccg
      181 gatcaggcga agtatgtgcg cgtttccgtc aacaccaacg attacatcac tgttcgcagc
      241 aaccagtttt cagaggtact gaacatcaat cccgccgcgg cgcgaaccaa ctattccggt
      301 aagccgagat tcatcgaaca tttttccaat cagttcctcc tgcgtctcga caagagaacc
      361 cggggtgaag agttgttgtc ttctttgaat ccattctccc ggcgcctcga cgacagcctc
      421 ctggtgacaa tgaactccag ctaccggaac atgttcttca tcaaccggaa cgatccggtg
      481 tggggcatgg actacacctg gcagcagaat cgtaacaagt cgttcctttc gaacggattc
      541 gaatcgcgca cactcacgac tcaatccatc aacggacgct ggaacatgag tcagcacttc
      601 cagctttcag gaactgccga acaggaactg cgggaaaaca actcggaata cttcaagacc
      661 cgcaactggc ggatcttgtc aaacgccctg gagccgcgca tcagctatca acgcggggta
      721 gattttcgta ctaccctctc ctatcggtat accgaaaagc gcaatttgta cggggacacc
      781 ggcgaaaaag ccgtcctgca caagttggga atcgacagcc ggctcaactc cgtcaatgcc
      841 ggtactttta acgcgcgttt caacgtcatc cgcattgact acaatgctcc cgacaacagt
      901 caattggcct acgaattatt ggaaggcttg aaagacggcg acaactatac ctggggtctt
      961 tccctgctgc gttctcttgg aaaatcgatg cagcttacgc tgaattacga cgggcggaag
     1021 tcgaccggaa gtccggtcgt acataccggc ggtgttcagt tccgggcatt cttctgagcg
     1081 tgacaaaaaa aagcccgcca aacggcaggc tttggttatt attcgcttca ggcttacttg
     1141 agatcgatcc ctgatttccc gaccaccagt cctgcgtggt agatctcgac cgtgtacttt
     1201 cctttgggga attccgaccc ttttgcccag cttacttcca ccggcgtgtc ggagttggtg
     1261 tattccaccg tttgcttcag ggtaaagttc agatcgttgc cgttggcctt aaaggtctcg
     1321 gtagaagccg acatgaccgc gccatcggga ccaatgaccc ggatgtagag atcgaccgga
     1381 ccgcgatcga tgaccttgtt ttccgccagc gtgaatttgg ttcggatctt ctgcacctgc
     1441 ttagccttgt tggtgagcac ttcctttccc gtactgcgga accggacgcc ttcggtggta
     1501 acattcaggg ccttcaggac cgagccggca gctactttag tggacaaccg gttgttctcg
     1561 ctactaagat tctcggaacg ggaacgctct tcaagcaagt tggagttcag gttcatgttc
     1621 tccgcctgca gctgggcatt caccttgttc agcgagtcga tctgaaccgt gtaaacctgg
     1681 ttcatctcct tcaactttgc caactccgcc ttggcccgcg cgatctgggc cggaccaccc
     1741 aacgcgatca ggcgctggat ctcggccatt ttggatttga tctcctcatc gcgctccgcg
     1801 agctgcgact gaaggccggc attgtcctgt ttgatcttct cgtactccgc ccgaacctgg
     1861 ttcaactggt tctgcaacgc gtccttctcg gccgtggtgc tgaggatggt ctggttggcg
     1921 gcatcgaggc tgttcttttt ctcgaagaac tgccacaaca agagaccgtt ggtgcccaag
     1981 agaatggtga tcacaatgat cacgatcccc ttcttggggc cttgttcctg gtaatcggtc
     2041 ttgttggcgt cttgtgaatc catggattcg tgtggttagg ttgggtcagt tagcgctgat
     2101 cttgtcttta ttgacttcgt cgacgtaatt caggcggagc tcggtaagga ccccgtccaa
     2161 catacgttgt tcctcgtcgg tcagtcttcc gctggtcttg tctttcaaca tttccaacat
     2221 ttcaatcgac tgcttggcct gtcccaggtc gcgttcgatc ttatcggtag ccggattctt
     2281 cagctttccg agtgcgacca ttccggagga atgaaagata taaatcagct gaacgaaagg
     2341 aggcagctgc atgttacttt tggtgttaat tagcgtaaaa aacaaaggta atattttttt
     2401 aaagcaggca aagtttgtct tgcccggcac ttattacctt ttgcaacact catcatcgga
     2461 ccaaaatcgg atatggcggc atcgaggaaa aagtacagcc tgcgcaaccg tgtagccttg
     2521 ctgcgcagtg gcgaacgcta cttcgaggtg ctgttgcgga tgatcgcgga tgcccggcag
     2581 gaatttcatc tgcagaccta catcctggag cccgatacca ccggccgtct tgtgctggaa
     2641 gccttgatcg ctgcagcggc aagaggggtc gccgtttaca tccagttgga tgcatacgga
     2701 tcaggtaaat tggatgaaac aatgttgtcg gaccttcgaa gca
//
LOCUS       DAOTON010000352         2735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_28967_length_2735_cov_3.092910, whole genome shotgun sequence.
ACCESSION   DAOTON010000352 DAOTON010000000
VERSION     DAOTON010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28967_length_2735_cov_3.092910"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /locus_tag="P5292_07590"
     CDS             complement(<1..1667)
                     /locus_tag="P5292_07590"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39029.1"
                     /translation="MSRFSLFLGVLLFSSLLSSESYAQVFSQDFNSSTTVSTYVNATT
                     PSTGQFNAITAGSGSSVSASSNNLAFTRGSGACSFSRSTSFSPVPSAIVYKFDLRVSG
                     TPSTNATTPATWRVGTGFGTGNSDENDNNTFARMSIDFRGTSNFRFNDVSNNATSSNY
                     SVGTTYTITWVLNNSGSTRTYISPSGTSESVANNRIDFWVGTTRVWNDVNAEDSGDPL
                     EDLKFYYGSSTGTITMDNINIQELVGVGTQPSNQSVCADPGTVSFTAASSPSGAAVQW
                     QVSTNGGSSWSNISGATSATYSFTAVTGDNGNQYRAIFNGLVATNAATLTVNANPSAP
                     TATPSGNTICGGQGAGVALSISPAISGTTYSWPAPTLSGVTGGTSASSQTSLNQILTN
                     NNTVAGTAVYTISATTPAGCTSASTTVVTITVNPSPVATATPSSQAFCGSSATTNIDL
                     TSSLPGTSYTWAVSQAGGVTGSSACASGCGTNISQTLSNAGGTSGTATYTVTPTDGTC
                     TGSTITVVVTLTPTPVGNASNLTPAVCSGTGAGVTLTSSVTGTTYAWA"
     gene            complement(2165..2590)
                     /locus_tag="P5292_07595"
     CDS             complement(2165..2590)
                     /locus_tag="P5292_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39030.1"
                     /translation="MIRLSMSDLRQQLKLIVFLFMMSLLQPMAAQDLDARSPKPMPLG
                     REQKKADKKKAKQAAELDKAIRKGRKQHEKLQSKQTKKMMKKSRKKSKRWNDNRREFF
                     LKRWFSPRHRKADAGFLPEYYAFYGRARRVETLFDPFYV"
BASE COUNT          686 a          683 c          747 g          619 t
ORIGIN      
        1 gcccaggcat aagtagtacc ggtgacagaa ctggtcaggg ttacgccggc tccggtacct
       61 gaacaaacag cgggcgtgag gttggaggca tttccaaccg gagtaggtgt cagggtaacg
      121 accaccgtaa tcgtggaacc cgtacaagtt ccatcggtag gcgtcactgt gtaagtggca
      181 gtgcccgacg tcccacctgc attggaaagg gtctggctga tgttagttcc gcaacctgat
      241 gcacaggcag atgaacctgt cacaccaccc gcctgagaga cggcccaggt gtaactggta
      301 cccggaaggg aactggtcag atcgatgttc gtagtagccg acgatccgca gaaagcctgc
      361 gaggaaggag tagccgtagc cacaggactc ggattcaccg tgatggtgac taccgtagtc
      421 gaggcactcg tacaaccggc aggagtggtt gccgatatgg tatatactgc ggtaccggca
      481 accgtgttgt tgtttgtcag gatctgattg agactcgtct ggctgctggc cgacgtaccg
      541 cctgtgacac cagataatgt tggagccggc catgaatatg tcgtaccgga aattgccgga
      601 ctgatgctca aggcaacacc tgctccttgt ccgccgcata tcgtattgcc ggaaggcgtg
      661 gcagtaggtg cggaaggatt cgcatttacc gtaagggtag cggcgttcgt agcaacaagg
      721 ccattgaaaa tcgcgcgata ctgattgccg ttgtcgccgg tgacagctgt aaaggaatag
      781 gttgctgaag tggctccgga aatgttcgac cacgatgaac cgccgttggt agagacctgc
      841 cattgcacgg cagctccaga aggactgctt gcagcggtga acgatacggt ccctggatca
      901 gcacataccg actgattgga cggctgcgtg ccaacgccaa caagctcctg gatattgata
      961 ttatccatgg tgatcgttcc ggttgaactt ccgtaataga atttcaaatc ctccagggga
     1021 tccccgctat cttctgcgtt cacatcattc cagacgcggg tggtacccac ccagaagtcg
     1081 atacgattat ttgcaactga ttccgatgta ccgctgggag aaatataggt ccgggtagac
     1141 ccggaattat tcagcaccca ggtgatggtg taggtagttc ctacggaata gttggagctg
     1201 gtagcattat ttgaaacatc gttaaaacgg aagttcgaag tcccccggaa atcaatcgac
     1261 atgcgggcaa aagtgttgtt gtcgttttca tcggaattcc ccgtgccgaa tccggttcca
     1321 acacgccagg tggcgggagt ggtggcattt gtggacggtg ttcccgatac gcgaagatcg
     1381 aacttgtaga caatcgcgga cggaaccgga ctgaagctcg tactgcgaga aaacgaacaa
     1441 gcgcccgaac ctcttgtgaa ggccaggtta ttactcgagg cgctgacaga gctacctgaa
     1501 cctgccgtga tcgcgttgaa ttggccggta ctgggagtag tagcgtttac ataagtactg
     1561 accgtcgtcg aggagttaaa atcctgactg aacacttgcg cgtagctctc cgaagagagc
     1621 aaactggaaa acaacaatac gcccaggaag agtgagaacc tggacattgt cttaccggaa
     1681 gtcgagtatc cgtgtatcat gcgagagggg tttgtgtagt tgcgtatgta gtggctgacc
     1741 catcaacggc tcgtacattc gtaagagcaa tcgactgggc aagtggtttt ataagttttc
     1801 tatcgaaaaa agcagagggg gtataaagaa ttagttattt cagatggggc gatatggcgg
     1861 ttgataaggt tggagcaaat ggaatcctga atgaaagatg aatacaaaga aaccccctga
     1921 aaggcccatg aatcaagggt tttaggtgaa tgcacctaca gtttttggcc aacgcccgat
     1981 aaaacttggc caacaaacca acccgcttta ctggcaaatg ttaaatctcc gaaaaaggca
     2041 ataaccccgg aaaccagcca tggtggccgg tttcaaagca atacgacaaa tgacgtagga
     2101 atcagactcg ctgcatccac atccaaaacg cctatctcaa ggtgtaggta tgcccaggat
     2161 tggcttaaac gtaaaagggg tcaaacaagg tctccacgcg tctggcgcga ccataaaagg
     2221 cataatactc aggcaagaaa ccggcatccg ccttccgatg gcgcggcgaa aaccaacgct
     2281 tcagaaaaaa ctcccgccgg ttgtcgttcc atcgcttcga tttcttccgg cttttcttca
     2341 tcatcttctt cgtttgcttc gactgcaact tttcatgttg cttccgtccc ttgcggatgg
     2401 ctttgtcgag ttcggctgcc tgcttcgcct tcttcttatc cgccttcttc tgctcccgtc
     2461 ccaacggcat gggcttcggc gaacgcgcat ccaggtcctg cgccgccatc ggttgcaaca
     2521 acgacatcat gaaaaggaaa acgatcagct tcagttgctg gcgtaaatcg gacatcgaga
     2581 gacgtatcat ggatgtggta ataacaattg aacggccacc cggccggaac cgggatgacc
     2641 gttcatgggt agtgacaaca agattccttc aaccgctcga ttaactcttc ggctccagat
     2701 ccatcccgca ggtcggacat tttcccggct ggtcg
//
LOCUS       DAOTON010000353         2732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29013_length_2732_cov_3.008218, whole genome shotgun sequence.
ACCESSION   DAOTON010000353 DAOTON010000000
VERSION     DAOTON010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29013_length_2732_cov_3.008218"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..671
                     /locus_tag="P5292_07600"
     CDS             150..671
                     /locus_tag="P5292_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRS39031.1"
                     /translation="MSNFLNRAFSIRSRWILFGSLLFSLGVLQACSGGETKESPASES
                     TEASSDGNLMADAENMKDDGKGIGPVTSVEVGAAVDAAMASEGEKLFEAKCTACHKVT
                     DEKYVGPGLKGVTQRRKPEWIMNMILNPTEMTQKDPAAKELLATHLTQMTNQNVTQED
                     ARKLLEYLRKIDG"
     gene            733..2685
                     /gene="nosZ"
                     /locus_tag="P5292_07605"
     CDS             733..2685
                     /gene="nosZ"
                     /locus_tag="P5292_07605"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HRS39032.1"
                     /translation="MKFNRWPVVLPALFALALMSCGGPKEKKSVAAGDAPSKVYVAPG
                     KLDEYYEFMSGGFSGQVGVYGLPSCRLLKVIPVFAVNAENGYGFTEESKAMLNSTHGF
                     VPWDDSHHPELSKTNGETDGRWLFINSNNTPRVARIDLSTFRTVEMLEIPNSGGNHAS
                     PFATENTEYVVAATRFSVPITGNKDVPISTYKENFQGSISFIKVAPEGDMSIAFQILM
                     PGFDFDLSHCGRGPSEGWFFFSCYNTERAHTLLEANASQNDKDFMVALNWKKAEEYAK
                     AGKGKEMNSAYYHNKLDEKTHTVKSEILNKTIMLDPKECTDMVYLMPCPKSPHGADVD
                     PSGEYIVGGGKLATVIPVFAFSKIQKAIANKAYAGEVDGIPVLKYEEVIGGEVQKPGL
                     GPLHTEFDGKGFAYTSHFVSSEVVKWKLGTWEVVDRIPTYYSIGHLSIPGGDTKSPSP
                     KYLIAMNKITKDRYLLTGPELNQSAQLIDLTGDKMKILYDFPTVGEPHYAQGIEADKV
                     MKHQVKVFKLEDNQHPYVAKSEKESRVERKGKAVHVYMTSIRSHFAPDNIEGVFVGDT
                     VYFHVTNLEQDWDTPHGFAIKGSTSSESLIMPGETQTIKFVAKTPDIYPYYCTDFCSA
                     LHQEMQGYLRVSSPGSNVPLTYSTGK"
BASE COUNT          686 a          708 c          713 g          625 t
ORIGIN      
        1 gttccgggct ctcaaaatat cggtatcttt tgtcgtcggt ttaaatgtag atattttcct
       61 gacaaatatc acctcgtgtt taaggctcct gtcagtcttc gttggtgata tttttggcta
      121 cttttgagcc gaaatcaaac tactaaacta tgtcaaattt cctcaatcgc gctttcagta
      181 tccgtagccg gtggatacta tttggtagcc tgctcttttc attaggagta cttcaggcct
      241 gttccggcgg agaaacaaaa gagagtcctg cttccgagag taccgaagcg tcctctgatg
      301 gtaacctgat ggctgacgcc gagaatatga aggacgacgg caagggaatc ggacccgtta
      361 ccagtgtaga agttggggct gccgtcgatg ctgccatggc atcggaaggg gagaagctct
      421 tcgaagcgaa gtgtacagcc tgccataagg taaccgatga gaaatacgtc ggtcctggtt
      481 tgaaaggcgt gacgcagcgc cgcaagccgg aatggatcat gaacatgatc ctgaacccga
      541 ccgagatgac gcagaaggat ccggcagcca aggagttgct tgctactcac ctgactcaga
      601 tgacgaacca gaacgtcaca caggaagatg ccagaaagct gctagaatat cttcgtaaga
      661 tcgacggctg atccccaccc aggatcgatc gttccattca ttcacccaca caaattcatc
      721 aggaggataa ccatgaaatt caatcgttgg ccggtcgtat tgccggcact gttcgccctc
      781 gcgctcatga gctgcggcgg gccaaaagag aaaaaatctg tcgcagccgg tgacgcgcct
      841 tcaaaggtgt atgtcgctcc gggtaaactc gatgaatatt acgagttcat gtcgggcggt
      901 ttcagcgggc aggtcggtgt ttacggactg ccatcctgcc gtctgttgaa agtgattccg
      961 gtatttgccg tgaatgctga gaacggctac ggtttcaccg aggaaagcaa ggcgatgttg
     1021 aattcgaccc acggattcgt tccctgggat gattcgcacc acccggaatt gtcgaagacc
     1081 aatggtgaga ccgacggtcg ctggctcttt atcaattcca acaacacccc gcgtgttgcc
     1141 cgcatcgacc tctcgacctt ccgcacggtg gaaatgctcg agattcccaa cagcggcggt
     1201 aaccacgcct ccccattcgc tacggagaac accgagtatg tagtcgccgc tacgcgcttc
     1261 tcggttccga tcactgggaa caaggacgtg ccgatcagca cctataagga aaactttcag
     1321 ggttcaattt ccttcatcaa ggtggcgccg gagggcgata tgagtatcgc tttccagatt
     1381 ctcatgcctg gtttcgactt tgatctcagt cactgtggac gcggaccatc cgagggttgg
     1441 ttcttcttct cctgctacaa tacggaaaga gcacacaccc tcctggaagc caatgccagc
     1501 cagaacgaca aggatttcat ggttgcgctg aactggaaaa aagctgaaga atatgcaaaa
     1561 gcaggtaagg gcaaagagat gaactcggcc tattatcata acaagcttga cgaaaagacg
     1621 cataccgtaa aatccgagat cctgaacaag acgatcatgc tcgatccgaa agagtgtacg
     1681 gatatggttt acctcatgcc gtgcccgaaa tccccgcacg gagctgacgt ggatccgagt
     1741 ggagaataca tcgtcggtgg tggcaaactt gccacggtta tcccggtctt cgcattctcc
     1801 aagatccaga aggcgatcgc caacaaggct tacgccggtg aagtggatgg tatcccggtc
     1861 ctgaagtacg aggaagtgat cggcggagaa gtccagaagc ccggccttgg tccgttgcat
     1921 accgaattcg acggaaaagg atttgcctat acttctcact tcgtatcttc cgaagtggtg
     1981 aaatggaaac tcggcacatg ggaagtggtt gatcgcattc cgacttacta ttccatcggt
     2041 cacctttcaa ttcccggtgg cgataccaag tcgccttctc cgaagtacct cattgccatg
     2101 aacaagatca ccaaggatcg ttacctgctt acgggtccgg aattgaacca aagcgcacag
     2161 ctgatcgacc tcacgggtga taagatgaag atcctttatg atttcccgac ggtaggcgaa
     2221 ccgcactatg cccagggtat cgaagccgat aaggtgatga agcaccaggt caaggtgttc
     2281 aagctggaag acaatcagca tccgtatgtc gcgaagagcg agaaagagtc gcgcgtcgag
     2341 cgtaaaggga aggcggtcca tgtttatatg acctccattc gttcccactt cgctccggat
     2401 aacatcgaag gtgtatttgt cggcgatacg gtctatttcc acgtaaccaa cctcgaacag
     2461 gattgggata cgccgcacgg attcgccatc aagggttcga cctcttccga gtcgctcatc
     2521 atgccgggcg agacgcaaac catcaagttc gtagcgaaga cgcctgatat ctatccgtat
     2581 tactgtacgg acttctgttc cgcattgcac caggaaatgc agggttatct ccgggtatcc
     2641 tcccctggtt ccaacgtacc cctcacttat tccaccggca aataaggaga gagagggcga
     2701 cgagggggga agcgattccc ccctcttgct tt
//
LOCUS       DAOTON010000354         2731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29024_length_2731_cov_2.732063, whole genome shotgun sequence.
ACCESSION   DAOTON010000354 DAOTON010000000
VERSION     DAOTON010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29024_length_2731_cov_2.732063"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="P5292_07610"
     CDS             complement(<1..506)
                     /locus_tag="P5292_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39033.1"
                     /translation="MKRTLCFLLVALLYILQVSAQDTLVYRNGKREVARVLEVGEFTI
                     RYNLLGTSNEPDRAVSRLDLQSIHFQNGKIEQFERPSRSDDPLLVRRLRMEEELGDSE
                     WAIFGEIGGSGILSVNGDYRIYKDALAPLAVHIRIGAGIYPDKDPEFTIPITASVVTG
                     RLYKFEMG"
     gene            complement(503..868)
                     /locus_tag="P5292_07615"
     CDS             complement(503..868)
                     /locus_tag="P5292_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39034.1"
                     /translation="MRRSRRNCSYSSESLLSFVDRQPMEKAWIPYLLLAFLAAVIMLR
                     IAKAKRSGLPALKVELFSLIVYAFFLGTFFLFLLMQLGDSWIPLLLIGTLSAGFFFRA
                     YPVFRMLDAYDPEKRDRSS"
     gene            954..1868
                     /locus_tag="P5292_07620"
     CDS             954..1868
                     /locus_tag="P5292_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRS39035.1"
                     /translation="MQELAPVLITGGSGLIGSAITEALTHNRIPVRWYSRQKLQPSSG
                     VQVFEWNPANGSADAAALTGVETIIHLAGAGIADERWSPARKALIINSRVQAANTLFE
                     LLRTHPHQVKTIVAASAVGYYGDRGEQLVDEAAAPGRGFLSDSCVAWERALSRFSELG
                     IRIVILRVGFVLSRQGGALPVMRKPVVWLLGTPLGSGKQYISWIDLDDLVGLFRFAMD
                     HHQLRGTYNAVAPAPVTNRQLVFTIGKLLHRPVWPIPVPGFLLQLLLGEKAVLVTGGQ
                     RVSADKIQRAGYHFHFPALEASLRHQLL"
     gene            1865..>2731
                     /locus_tag="P5292_07625"
     CDS             1865..>2731
                     /locus_tag="P5292_07625"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRS39036.1"
                     /translation="MKEHLTFFWFRRDLRLHDNAGLYHALRSGLPVQPVFIFDRNILD
                     ALEDRSDRRVVFIHQQLERLQKELKQLGATLLVGYGRPADCWKRWSEEYSIRSVFTNH
                     DYEPYATRRDSELRQFFDERGISFHTFKDQVIFEKSEVMKDDGSPYTVFTPYMKKWRS
                     LIRPFFLKSYPTERYTANFLQRDPLQLPSLKEMGFHEVECAFPESTVSTDLLRDYADK
                     RDIPGIRGTSRLSVHLRFGTISIRDLVREAWSVSEKWVNELIWREFYQMILWHFPHVT
                     ERSFKAQYDLLEW"
BASE COUNT          603 a          784 c          723 g          621 t
ORIGIN      
        1 catcccattt cgaatttgta caagcggccg gtaaccaccg aagctgtgat cgggatcgtg
       61 aattccgggt ctttatccgg ataaatcccg gccccgatcc gaatatggac cgccagcggg
      121 gccaaggcat ctttatagat ccggtaatcg ccattgaccg aaaggatgcc cgaaccgccg
      181 atttcaccaa aaattgccca ttctgaatca cccaactcct cttccatacg caaacggcga
      241 actaacaacg ggtcgtccga acggctgggt cgttcaaact gctctatttt accgttttga
      301 aaatgaatgg attgtaggtc tagacggctg actgcccggt ccggctcgtt cgatgtcccc
      361 aggagattat agcgaatggt gaattccccg acttccagta ccctggccac ctcccgctta
      421 ccattccggt aaacgagcgt atcctgtgct gatacctgaa gaatatacag caaggcgacc
      481 aggaggaaac aaagggtacg tttcatgacg aacggtctcg tttttccgga tcatacgcgt
      541 ccaacatccg gaacaccgga taagcccgga agaagaagcc ggccgaaagt gttccaatga
      601 gcaacagcgg gatccaactg tcgcccaatt gcatcaagag gaaaaggaaa aatgtgccga
      661 ggaaaaaggc atagacgatc aggctgaaca gttcgacttt cagggccgga agtccgcttc
      721 gcttcgcttt ggcaattcgc aacatgatca ccgcggccag aaaggccaac aataaataag
      781 gtatccaggc tttttccatg ggctgacgat cgacaaagct aagcagagat tcggaggagt
      841 acgaacagtt ccgcctgctt cgtctcattc tgatcatcgt atgattattc cacaattatt
      901 caggcattca gcagggacgg aaacctttcg gcacctttgt ttgtcttctt accgtgcaag
      961 aactggcacc cgtactcatc accggcggat ccggcctcat cgggtccgcg atcaccgaag
     1021 ccttgaccca caaccggatt ccggtgcgct ggtatagccg gcaaaagctt cagccgtcct
     1081 ccggcgtgca ggtgttcgaa tggaatcctg ccaacggtag tgcagatgct gccgcgctca
     1141 ccggcgtcga gacgatcatc cacctggccg gagccggcat agcggacgag cgttggtcgc
     1201 ctgccagaaa agcgttgatc atcaacagtc gcgttcaggc tgcgaacacc ttgttcgaat
     1261 tacttcgtac gcatcctcac caggtcaaaa cgatcgtcgc ggcttcggca gtcggatatt
     1321 acggtgatcg cggcgagcaa ctcgtcgacg aagcggcggc acccggccga ggatttctct
     1381 ccgactcctg cgtcgcctgg gagcgggcgc tttcccgctt ttcggaactg ggaatccgga
     1441 tcgtgatcct gcgcgtcggt ttcgtgctca gtcggcaggg aggagccctt ccggtgatgc
     1501 gcaaaccggt cgtttggttg ctgggcactc cacttggttc aggaaagcaa tacatctcct
     1561 ggatcgacct cgacgatctg gtcggcttgt tccgcttcgc gatggaccat caccagcttc
     1621 ggggtaccta taatgctgtt gcgcctgcgc cggtcaccaa ccgacaactg gtattcacga
     1681 tcgggaagct actgcaccgt ccggtctggc cgattccggt tccgggattt ctcctgcaac
     1741 tgctactcgg cgagaaagcc gtgcttgtca ctggtggcca gcgtgtttcg gccgataaga
     1801 ttcaaagggc cggctatcac tttcactttc ctgcgctcga agcttcgctg cgccatcaac
     1861 tcctatgaaa gagcatctga cgtttttctg gtttcgccgc gacctccgcc tgcacgataa
     1921 cgccggtctt taccacgcct tacgttccgg tcttccggta cagccggtgt tcatcttcga
     1981 ccgcaacatc ctggatgcgc tcgaggaccg ttcggaccgt cgggttgttt tcatccatca
     2041 gcaactggaa cggctgcaga aagaactgaa gcaattgggc gcgaccctgc tcgttggcta
     2101 tggtcgtccg gcggattgct ggaagcgctg gtcggaagag tattcgatcc gcagtgtttt
     2161 tacgaatcac gattacgagc cctacgccac ccggcgcgac agcgagctca ggcagttctt
     2221 cgacgaacgc gggatcagct ttcacacctt caaggaccag gtgatctttg aaaaatcgga
     2281 agtgatgaaa gacgacggat cgccgtacac ggtctttact ccgtacatga agaaatggcg
     2341 ttcgctcatt cgtcccttct tcttgaaatc ctatccgact gaacgctaca ctgccaattt
     2401 cctgcaacgc gatcctttgc aactgccttc gctgaaagag atgggttttc atgaagtgga
     2461 gtgcgcgttt ccggaatcca ccgtatcaac cgatctcctg cgtgactatg cggacaagcg
     2521 cgacatcccc ggcattcgtg gtacctcgcg cctcagtgtg cacctgcgct tcggtacgat
     2581 cagtattcgc gacctcgtcc gggaagcatg gtcggtgagc gagaaatggg tgaatgaact
     2641 catctggcgc gagttctacc agatgatcct ctggcatttt ccgcatgtca cggagcgttc
     2701 attcaaagcg cagtatgacc tgctcgaatg g
//
LOCUS       DAOTON010000355         2721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29142_length_2721_cov_3.229557, whole genome shotgun sequence.
ACCESSION   DAOTON010000355 DAOTON010000000
VERSION     DAOTON010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29142_length_2721_cov_3.229557"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            683..>1952
                     /locus_tag="P5292_07630"
     CDS             683..>1952
                     /locus_tag="P5292_07630"
                     /inference="COORDINATES: protein motif:HMM:NF017889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007160 - cell-matrix adhesion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nidogen-like domain-containing protein"
                     /protein_id="HRS39037.1"
                     /translation="MTKRVTLLIGVILFFASPLFSQVDVKTVDMRKINQAKLDGKLTG
                     REQFVNYDALGKNPARVAENVNPGNSVNTTNGCACWIPRDSTWQVAQFDGSGGSGGPG
                     LPPDYRNDDWSTVAIPLGFPFAFYDVQLYEVYINNNGNVSIGAPYATFTANSFPDPNY
                     SMIAPFWADVDTRGPLSGIVYYQLTNSHLIVQWENVGYFSQHDSLGNTFQLILTNGWD
                     PLLPPGQNVGFCYQDMQWTTGDASGGSGGFGGTPATVGVNQGNGTDYIQIGLFDQTGA
                     SYDGPYGNNDGIDALDNQSFVFNLAQSGSNVPPILNSAQACDTLRVCAGDTLLIEGVF
                     LSPEQGQITTVSTSTTMSGVTVTSLPGNTANFTVQVVAQASNLGLNNLMIIATDDGTP
                     VGASQFPVVIDVQPSPVATVTSTNISCNGAN"
     assembly_gap    1953..1962
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1963..>2721
                     /locus_tag="P5292_07635"
     CDS             <1963..>2721
                     /locus_tag="P5292_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39038.1"
                     /translation="ITGSGPFDILWTPGGYQTASVSNLAAGTYTVDISIPAGCSTTNQ
                     VTITEPAALSISMTAQDVNCSGQTGSVVCTVGGGTGPYDYLWNTTPPQTTSQITNLTA
                     GTYTVDVTDANNCTISATAEVHGLAGFSATISTTPATCLASDGTASVQTTGGSGNFSY
                     SWNPNVSTGATATGLAAGMYEVTITDNADGCQQIVTGTVANSSGIVASIVSSSGATCQ
                     NGEDGSATASGSGGTAPYSYLWMPNGDTTATVNNL"
BASE COUNT          531 a          870 c          709 g          601 t
ORIGIN      
        1 cgcaactcgt accatttttt cctcggtcac ggtctcgttc ctcgtaaccc caccttttca
       61 cctccctcct tcatagtcga ctgttgttgc gcccctccgg ggctttattc gtgggagaca
      121 ttctcctgtc tattatgttt ggcccctccg gggctgggtg ttgggtgaat cgtacagaat
      181 tgattggtgt gttcagtttg cagtttgcag attacacaca tcccgcacct cgtacctcgc
      241 atctttttct cccggtctcg gtcacgctcc ttgcaactca cctcgcgggc ttgaaaccac
      301 cgaggacgca gaggttttca caggacacga aggtcagaag gcagaagcca gtagccagta
      361 cgcagatagc aggttcctcg cgcctatttt tcctcggtca cggtctcgtt cctcgttccc
      421 tcttttccac gtccctcgtc gctcgcccct cgcccctcgt ccctaacccc cgcacctcca
      481 tttcctcggt cacggtctcg ttcctcgttc ctccattttc ctcgtccctc atttgttaat
      541 gtccgaaaac ccttgatttt actgcctttc cggggttttg ttcgcggaat atttttgcgg
      601 cattcggggg cctttggtaa attgatgatc taaatccgct gtcaccacag cccaattcca
      661 tttatctctt atagtagtag tcatgacaaa acgagttacg ttactcattg gtgtcatctt
      721 gttctttgca tcgccgctgt tttcccaggt cgatgtgaaa accgttgaca tgcgaaaaat
      781 caatcaggca aaactggacg ggaaacttac cggcagggag cagttcgtca actacgacgc
      841 tctcggaaag aacccggccc gtgttgccga gaatgtcaac ccgggaaatt cggtaaacac
      901 gaccaacggt tgtgcctgct ggatcccccg cgattcaacc tggcaggttg cgcagttcga
      961 cggttcgggc ggcagcggcg gtccgggtct tccgcccgac taccgcaacg acgactggtc
     1021 gaccgtcgcg atcccgctcg ggtttccgtt cgcgttctat gacgtgcagt tgtacgaggt
     1081 gtacatcaac aacaacggca acgtttccat cggcgctcct tatgcgactt tcacagccaa
     1141 ctcgtttccg gatccgaact attcgatgat cgcgcccttc tgggccgacg tcgatacccg
     1201 cggtccgctg agcggcatcg tgtactatca actgaccaac tcgcacctga tcgttcagtg
     1261 ggagaatgtt ggttatttca gtcagcacga cagcctgggc aacaccttcc agctcatcct
     1321 gaccaacggt tgggatcccc tgctgccgcc cggacaaaac gtcggcttct gttatcagga
     1381 catgcaatgg accaccggtg acgcttcagg cggttccggc ggttttggtg gtacgcccgc
     1441 gacggttggc gtgaaccagg gtaatggcac cgactacatt cagatcggat tgttcgacca
     1501 gaccggtgct tcgtacgacg gaccctacgg caacaacgac ggtatcgacg cgctcgacaa
     1561 ccagtctttc gttttcaacc tggcacaaag cggcagcaac gtgcccccga tcctgaactc
     1621 ggcacaggcc tgtgatacgc tgcgcgtctg cgccggcgat acgcttttaa tcgaaggcgt
     1681 gttcctctct ccggagcagg gccagatcac cacggtttcc acttccacca ccatgagcgg
     1741 cgttacggtg acttcgttgc cgggcaatac cgccaacttc acggtgcagg tcgtcgccca
     1801 ggcgtcgaac ctcggactca acaacctcat gatcatcgcg acggacgatg gtacgccggt
     1861 cggcgcatcg cagttcccgg tggtgatcga cgtgcagccc tcgccggtgg ccacggttac
     1921 ctcgaccaac atcagttgca acggcgccaa cgnnnnnnnn nnattacggg cagcggtccg
     1981 ttcgatattc tctggacacc cggtggttac cagaccgcgt cggtcagcaa cctggctgca
     2041 ggaacctata cggtcgatat cagcattccg gccggttgtt cgaccacgaa ccaggtgacc
     2101 atcaccgaac cggcggctct atcgatctcg atgacggcgc aggatgtcaa ctgcagcggc
     2161 cagaccggca gcgtagtctg tacggtcggt ggcggcacgg gtccttacga ctacctgtgg
     2221 aacaccacgc ctccgcagac caccagccag atcaccaacc tgacggcagg cacctacacc
     2281 gtcgatgtaa ccgacgcgaa caactgcacg atctccgcca cggcggaagt gcacggcctc
     2341 gccggcttca gcgcgacgat cagcaccacg cctgcgacct gtctggcttc cgacggtacg
     2401 gcgagtgtgc agaccaccgg cggtagcggc aacttcagct actcctggaa cccgaatgta
     2461 agcaccggcg ccaccgctac gggactggct gcgggcatgt acgaagtcac gattaccgac
     2521 aatgccgacg gctgtcagca aatcgtgacg ggcacagtag ccaacagctc cggcatcgtc
     2581 gccagcatcg tttcctcttc cggcgcgacc tgtcagaacg gtgaagacgg atccgccacg
     2641 gccagcggtt cgggcggaac agctccctac tcgtacctct ggatgccgaa cggcgacacc
     2701 accgctacgg tgaacaacct g
//
LOCUS       DAOTON010000356         2705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29366_length_2705_cov_3.089057, whole genome shotgun sequence.
ACCESSION   DAOTON010000356 DAOTON010000000
VERSION     DAOTON010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29366_length_2705_cov_3.089057"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2705)
                     /locus_tag="P5292_07640"
     CDS             complement(<1..>2705)
                     /locus_tag="P5292_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRS39039.1"
                     /translation="PVIVYPVPTPDLGPDPAYCDGGSATFGTSGGVSYDWTPGGMTDS
                     VITVNPAATTAYTVTVTNAQGCTASDSILVTVYPLPIADAGTNQAVCFGTSATLTASG
                     GVLYDWQPIGVGTATTTITPDTTSDYTVLVTDANGCTDTDVVTVVVNELPVLSATADS
                     ARCNGAADGVATSQVTGGTGPYGYTWSPSGGSAAVAGGLTAGAYSVIVTDVNGCSDTA
                     YAQVNEPAALTIAASATDVACNGFTNGTLNANAGGGIPGYTYTWSPGNHSGASISSLP
                     AGTYTVTATDYNGCTITTTTVIQQPTVLQANASAVPTLCNGSATGSVSASVNGGTAGY
                     TYNWLPGNATTANVSGLAAGLYNVTVLDANGCLTNASVNVTEPTPVSVSTGSTAATCG
                     VANGSVNALASGGVGGYTYSWTPGGGVGATVNGLYAGGYTVTATDANGCTSTQVATIS
                     NTGAPVITASVVQNVSCFGGADGSAVANIVSGNGPFTYSWPGGVANDTVSGLSAGTYP
                     VTVTDVNGCSALDTVQVIQPTALSIITGARPASCAGYSDGSVSFSVSGGTPGYQAVWS
                     PGGHTGNINSGIPAGVYSVTVTDAHGCTILGSATVTQPQPVSVSGSTAPVSCNGGSDG
                     QAFIAAAGGTGPYEFDWLPSGIANDTASGLTAGQHIVTITDSLGCITSDTLTVTQPDP
                     VTLTIVAAGVSCSGAADGVAAVTAAGGNGGYQYLWSNNATSASLNNVPAGLYTVTVTD
                     LLGCTATGSAIVDDPFPVELFVEGPAPICIGQEALLTASASGGTSPFSFTWNEGTVND
                     SLAVALDTTTTFSVVVTDANGCSTAPQEVEVVVYPPLQLSVAELPIICGGDSALLSAE
                     VTGGNGGPYAYSWNDGAINGASSVIQPLNDSLFVVMATDGCSPPV"
BASE COUNT          558 a          820 c          806 g          521 t
ORIGIN      
        1 taccggcgga ctacagccgt cggtcgccat cacgacaaag agcgaatcgt tcaagggttg
       61 gatgaccgag gatgcgccgt tgatggcacc atcgttccaa ctataagcat atggcccgcc
      121 gttaccaccg gtcacctccg cgctcaggag cgcggaatcg cctccgcaaa tgatcggaag
      181 ctccgccacg ctcagttgca aaggcggata gaccacgacc tcgacctcct gcggcgctgt
      241 cgagcatccg ttggcatcgg tcaccacgac ggaaaaggtc gttgtcgtat caagcgcaac
      301 agccagcgag tcgttgacgg ttccctcgtt ccaggtgaag ctgaacggac tcgtacctcc
      361 gctggccgat gcggtgagga gggcttcttg tccgatgcag atcggtgccg gtccttcgac
      421 gaacaattcc accggaaacg gatcatccac gatcgccgaa ccggtcgcgg tgcaacccag
      481 caggtcggtc accgtgacgg tgtacaatcc cgccggaaca ttgttcaggc ttgcgctggt
      541 cgcattgttc gaccagagat actgataacc cccattgccg ccggctgccg taacggccgc
      601 aacaccatcg gccgcgccgc tgcaagagac tcccgcagca acgatagtca gtgtaacggg
      661 atccggttgc gtaaccgtca gcgtatcgct ggtgatgcaa ccaaggctgt cggtgatggt
      721 gacgatgtgc tggcccgctg tcagtccgct cgcggtatcg ttggcaatgc ccgagggcag
      781 ccagtcgaac tcgtacggac ccgtcccgcc ggcagcggcg atgaaggctt gtccgtcgct
      841 accgccatta cacgatacag gcgcagtcga acccgacaca cttaccggtt gcggttgcgt
      901 gacagtcgcg ctgccgagta tcgtacagcc gtgcgcatcc gtaacggtga ccgaatatac
      961 tcctgcagga atccctgaat tgatgttgcc ggtatgtccg ccgggcgacc ataccgcctg
     1021 gtaacccgga gtgccgccgg aaacggagaa gctcacggaa ccatccgaat aacccgcgca
     1081 actggcagga cgtgcaccgg tgatgatcga caaggccgtg ggttggatca cctgtacggt
     1141 atccagtgcg ctgcaaccat tgacatcggt gaccgtcacc ggatacgttc ctgccgaaag
     1201 tccgcttacc gtatcattgg caacaccgcc cggccaggaa taggtgaacg gcccgttacc
     1261 tgaaacgatg ttggcgaccg cgcttccgtc cgctccgccg aagcaggaga cgttttgcac
     1321 aaccgaagcg gtgatgaccg gagcaccggt gttggagatg gtggctacct gcgtcgaggt
     1381 gcatccgttc gcgtcggtcg cggtaacggt ataaccgccc gcatacaaac cgttgaccgt
     1441 cgcgccgact ccgccgccgg gtgtccagct gtaagtatat ccgccaacac cgccggatgc
     1501 cagtgcattc acactgccgt tggccacacc gcaggtcgct gccgttgaac cggtgctgac
     1561 ggatacaggt gtcggttcgg tcacgttcac cgaagcatta gtcaaacaac cgttcgcgtc
     1621 aaggaccgtg acattgtata agccggcagc cagtccgctg acattggccg tcgtggcatt
     1681 tcccggaagc cagttgtagg tatagcctgc cgtaccgcca ttcacactcg cagagaccga
     1741 tccggtcgcg ctgccattac acaatgtcgg caccgcgctg gcgttggctt gcaataccgt
     1801 tggttgctgg atgaccgtag tggttgtaat ggtacaaccg ttatagtccg ttgcggttac
     1861 cgtataggtt ccggccggaa gggaagaaat acttgcgcca gaatgattcc ccggactcca
     1921 ggtgtaggta tatccgggaa ttcctccgcc tgcattcgcg ttaagcgttc cgttggtgaa
     1981 accgttgcag gccacatcgg tggcggacgc ggcgatcgtc aaagccgctg gctcatttac
     2041 ctgagcatag gcggtatccg aacaaccgtt gacatccgta acgatgaccg aataggcacc
     2101 ggccgtcaga ccaccagcca cggcagcact tccgccggag ggtgaccagg tataaccata
     2161 tggtccggtt ccaccagtga cctgactcgt cgcaacacca tcggccgcac cgttgcagcg
     2221 ggcggaatcg gcggttgccg agagaacggg caattcgttc actactaccg tgaccacatc
     2281 cgtatcggtg catccgttcg catcggtcac gaggaccgta taatccgatg tcgtatcggg
     2341 ggtgatggtg gtggtcgcag tgccgacccc aattggttgc caatcgtaaa gaacgcctcc
     2401 gctggcggtg agtgtggcgg aggtgccgaa gcagactgcc tgattcgtgc cggcatccgc
     2461 gatcggcagc ggataaacgg taacaagtat ggaatcgctg gccgtgcaac cctgcgcatt
     2521 cgtaacggta acagtataag cggtggtggc ggcgggattg acggtgatca ccgaatccgt
     2581 catgccgccc ggtgtccaat catagctgac gccgccgctg gttccaaacg ttgcgcttcc
     2641 gccatcgcaa tacgcggggt ccggacccag atcgggagtc ggcaccggat agacgatcac
     2701 gggct
//
LOCUS       DAOTON010000357         2705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29367_length_2705_cov_2.944151, whole genome shotgun sequence.
ACCESSION   DAOTON010000357 DAOTON010000000
VERSION     DAOTON010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29367_length_2705_cov_2.944151"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="P5292_07645"
     CDS             <1..381
                     /locus_tag="P5292_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HRS39040.1"
                     /translation="FSDLGAEDGETFLGLRELGFTDPVNVLPYGLDRISPPAGIPGAV
                     GATVNTVHGKETTIQEFRNREQADIETMEGAAFYYVCEREKVASLQLRGISNYVERRN
                     RANWKIAEALASLSRAAHYFIRKL"
     gene            384..1271
                     /locus_tag="P5292_07650"
     CDS             384..1271
                     /locus_tag="P5292_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HRS39041.1"
                     /translation="MRKLPDGTLQIDLAYSPCPNDTFMFDALVNGRIDTGPYRFDVHL
                     EDVETLNQNAFSEKRDLTKLSFFAFGKVIDRYILLDAGSALGNGVGPLLIARPGTKLQ
                     PGDETIVAIPGENTTANFLFSLYYPHLTRKQPMVFSAIEDAVLNRAVHAGVIIHENRF
                     TYEQRGLEKIRDLGEAWEQETGKPIPLGGIVAKRSLPATVLHDLNRLMAESVRYAFAH
                     PTAAADYVACHAQEMDPAVRQQHIDLYVNPYSENLGITGREAIRYLLLKAAATGILSV
                     NANDQLFISELQPSLTDNS"
     gene            1295..2260
                     /gene="rfaD"
                     /locus_tag="P5292_07655"
     CDS             1295..2260
                     /gene="rfaD"
                     /locus_tag="P5292_07655"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HRS39042.1"
                     /translation="MIVITGAAGFIGSCLVARLNEAGYNDLILVDDFSRLDKIRNLEG
                     KIFSQKVERSEFHDWLRKEHRFVQFVFHIGARTDTTEFRKEIFDELNVRYSQEVWNNC
                     VEFGLPLVYASSAATYGAGELGYSDDHAIVSKLHPLNPYGDSKNEFDQWALTQERAPY
                     FWAGLKFFNVYGPNEYHKGRMASVILHAFRQINSTGGMKLFRSHRPDYKDGEQLRDFV
                     YVKDVTNVCMFLMQHRKHPGLYNLGSGKARTFLDLAKSTFKAMGKPEHIEFIDTPADI
                     RDKYQYYTEADMRKLQSIGYDQPFYSLEAGVEDYVRQYLLADKTW"
     gene            complement(2267..>2705)
                     /locus_tag="P5292_07660"
     CDS             complement(2267..>2705)
                     /locus_tag="P5292_07660"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRS39043.1"
                     /translation="STLRITFGSVQAIQPRDGISYHWQTHLQGVLEKAATGEEDYRIP
                     QRLRSIARGEEQLPVAFLASNHTTGGNSGSPVLNARGELVGINFDRVWEGVMSDYDFA
                     PDFSRNISCDIRYILFVISKYGEAERLIREMDIRTGNPPAKKR"
BASE COUNT          626 a          761 c          700 g          618 t
ORIGIN      
        1 ttttctgatc tgggcgcgga agatggcgag acctttcttg gccttcgtga gctgggattt
       61 accgaccctg tgaacgtgtt accctatggg cttgaccgaa tttcccctcc agccggaatc
      121 cccggagcgg ttggcgctac cgttaatacg gtgcatggga aggagaccac cattcaggaa
      181 ttccggaacc gggaacaagc cgacatcgag acgatggaag gcgccgcgtt ctattacgtc
      241 tgcgaacggg aaaaggtcgc ttcacttcag ctccgcggca tctccaatta tgtggaacgc
      301 cgaaacaggg caaactggaa aatcgccgaa gcgctggcat ccttgtcgcg tgcagcacat
      361 tactttatcc ggaaactctg agcatgcgaa aacttcctga cggcaccttg caaatcgatc
      421 tggcttactc cccctgcccg aacgacacct ttatgttcga tgccctggtg aatggacgga
      481 ttgataccgg cccctaccgg ttcgatgttc atctggaaga tgtcgagacc ctgaaccaaa
      541 acgcgttttc cgaaaagcga gacctgacaa agctatcctt cttcgctttc ggcaaggtga
      601 tcgatcgtta tattctgctg gatgccggaa gtgcgcttgg aaatggcgta ggcccgcttc
      661 tcatcgcccg gccgggcacg aagctgcagc ctggcgatga aacgatcgtt gccatccccg
      721 gagaaaacac cacagccaat tttctgttca gcctgtatta cccgcatctt accagaaagc
      781 agcccatggt tttctccgcg atcgaagacg ctgttctgaa ccgagcggtg catgccggtg
      841 tcatcattca cgagaatcgc tttacgtacg aacaaagagg gctggaaaag atccgtgatc
      901 ttggagaggc ctgggaacag gaaaccggta aaccgattcc gctgggtggt attgtcgcaa
      961 agcgcagcct gcccgcaact gtgctgcacg acctgaatcg cctgatggcc gaaagtgttc
     1021 gctacgcatt cgcgcaccct acagccgccg ccgactacgt cgcctgccat gcccaggaaa
     1081 tggatccggc agttcggcag cagcacatcg acttatatgt caacccatac agtgaaaacc
     1141 tggggataac cggtcgtgaa gccattcgct acctgctgct aaaagcggct gccaccggca
     1201 ttctttccgt caacgccaat gatcagttgt tcatctccga gctgcaacca tcgttaacgg
     1261 ataattcctg aactttgacc tccacgtccg acctatgatc gtcatcaccg gtgccgccgg
     1321 attcatcggc agttgtcttg ttgcccgact caacgaagcc gggtataacg acttgattct
     1381 ggtcgatgat ttctcccgcc tcgacaaaat caggaatctt gaaggaaaga tcttcagcca
     1441 gaaagttgaa cgatcggaat tccacgactg gctgcggaag gaacatcgtt tcgtccagtt
     1501 cgtctttcac atcggcgcgc gtacggatac caccgaattc agaaaagaga tctttgatga
     1561 gctgaacgtt cgttattctc aggaagtttg gaataattgc gttgaattcg gcctcccgct
     1621 cgtttacgct tcctccgcag caacatacgg agcgggtgag ttgggttact cggacgatca
     1681 tgcgatcgta tccaaactac acccgctgaa cccgtacggc gattccaaga acgagttcga
     1741 tcaatgggcc ttgacacagg agcgcgcacc gtacttctgg gccgggctga agttcttcaa
     1801 cgtgtacggt ccgaacgaat accacaaagg acgcatggct tcggtcattc tccatgcctt
     1861 ccggcagatc aactccaccg gaggcatgaa gctctttcgc tcgcatcgcc ccgactacaa
     1921 agacggtgag caattacgcg atttcgtcta tgtgaaggat gtgaccaatg tctgcatgtt
     1981 cctcatgcag caccgcaagc acccgggctt gtacaatctt ggttcgggca aagcgcgcac
     2041 ttttctggat ctggcgaaga gcaccttcaa ggccatggga aaaccggagc acatcgaatt
     2101 catcgacacg cctgccgata tccgtgacaa gtaccaatat tacacggaag ccgatatgcg
     2161 aaagctgcaa tcgatcgggt acgatcagcc tttctattcg ctggaagcag gtgtcgagga
     2221 ctatgtgcgg cagtacctgt tagcggacaa gacctggtaa aataccttag cgcttctttg
     2281 ccggagggtt tccggtccgg atatccattt cccggatgag tcgttccgcc tctccatatt
     2341 tgctgatcac aaataggatg tagcgaatat cacacgagat gttccggctg aagtcgggtg
     2401 caaaatcgta atcgctcatc acgccttccc acacccggtc gaaattgatc ccaaccaatt
     2461 cgccccgtgc gttgagtacg ggcgaacccg aatttcctcc cgtcgtgtga ttgctggcca
     2521 ggaatgcgac gggtagttgc tcttcgcccc gtgcaatgga ccggagacgc tgcgggatgc
     2581 ggtaatcttc ttctccggtt gccgcctttt ccagtactcc ctgcaaatgg gtttgccaat
     2641 gataggaaat accatcccgc ggttgaatgg cctgcacgct tccgaaggtg atgcgcagcg
     2701 tactg
//
LOCUS       DAOTON010000358         2701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29440_length_2701_cov_3.215797, whole genome shotgun sequence.
ACCESSION   DAOTON010000358 DAOTON010000000
VERSION     DAOTON010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29440_length_2701_cov_3.215797"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="P5292_07665"
     CDS             <1..230
                     /locus_tag="P5292_07665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRS39044.1"
                     /translation="VLFFFLWIRASFPRYRYDQIMRLGWKIFIPVTLVFIVFLLFECV
                     TDNGIAHGQLQPGFLLPIEDQSGKQIKPANE"
     gene            complement(225..458)
                     /locus_tag="P5292_07670"
                     /pseudo
     CDS             complement(225..458)
                     /locus_tag="P5292_07670"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(481..678)
                     /locus_tag="P5292_07675"
     CDS             complement(481..678)
                     /locus_tag="P5292_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRS39045.1"
                     /translation="MSNEGKSFWAFLGGVAIGALLGILFAPAKGEETREKMADAARDW
                     SERAKRKAEDLRDTVNGKTAG"
     gene            complement(739..1566)
                     /locus_tag="P5292_07680"
     CDS             complement(739..1566)
                     /locus_tag="P5292_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRS39046.1"
                     /translation="MKSILVPTDFSDAALHAALFAASLARDTGTDLHLLNVYHLPNPL
                     KTLPIELIVTPDELKSDTESALKQIAQELRSSVGASLNITLASRNGETRRAINDYATY
                     AGAGLIVTGMKGKNAVQERLIGNTALELLRSSTVPVLSIPQTAEYRQLKTLLFATDGK
                     SIPDPESVTLLRNLLNHHPAGLHIGCVQSPHDNFDRDQLMTSLNPLLSGMQATWHFLS
                     DDDENTALEKLGREIGADWLALLPQKHSLFSRIFQQNHTRQLALHAGLPILALRNER"
     gene            1704..2567
                     /locus_tag="P5292_07685"
     CDS             1704..2567
                     /locus_tag="P5292_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRS39047.1"
                     /translation="MNQILCPTDFSPAAGHAVAYAAALSEQINARLVLLHAYESPVVF
                     SSAPLTSIRDAKQQIKLAAQKKLDKLVQQLEKIHPRLATETKLLAGLGSERILDAARD
                     IHADMIVMGATGTSKLSRLLIGSTTAAVIRDADCPVLCIPKEAKFNGINRIAFATDLQ
                     EDNLASAMTVTPFAAKFGAELSFIYVDDKHLLHSDEAIEEMTRRIRRRVKYPKLSGFI
                     AKNPSITKGLETFLKKQPADLLVMFTHPRRLGETLFNQSVTRMMSHQAKIPLLAIRRM
                     DQPVPSGERKR"
BASE COUNT          608 a          714 c          711 g          668 t
ORIGIN      
        1 ttgtcctgtt ctttttcctc tggatccgcg cgtcctttcc gcgctatcgc tatgaccaga
       61 tcatgcgtct gggctggaag atcttcattc ccgtcacgct cgtcttcatc gtgttcctcc
      121 tctttgagtg cgtgacggat aatggaattg ctcatgggca acttcagcca ggcttcctgc
      181 ttccaataga agatcagtcc ggcaagcaga taaaacccgc caacgagtaa gaagcccagc
      241 caggtctttc cgaagtgttc gccgagagcg aacgcggccg ctacgcttaa aaaaccgaaa
      301 accagcagga ataggaaaac aaggatcagg ttggcggtta cattgccggc aagttcggct
      361 cctttgtcga ttgcctgaag tttgctcagg cggctccgtg tttcaacgta atcctgaacg
      421 cggtcgatga tgcggtcgat cggatggtta tcttccatga tgagagggtt gatagaacgc
      481 ttagccggct gttttgccgt ttaccgtatc gcgcaggtct tccgcttttc gctttgcccg
      541 ctcgctccag tcgcgtgcgg catcagccat cttttcccgg gtctcttctc ctttggcagg
      601 agcgaagagg atgcccaaca acgcaccaat ggctaccccg ccaaggaacg cccaaaatga
      661 tttgccttcg ttactcatgc tgaggaattt agattaaaca aatataagca cattcaagtg
      721 tcagtggcat gagttagctc acctttcgtt tctcaatgcc aaaatcggca ggccggcatg
      781 cagggctagt tggcgcgtgt ggttttgctg gaaaatcctg ctgaataaac tgtgcttttg
      841 aggcaggagg gcgagccagt cggcaccgat ctcccggccg agcttttcaa gcgcggtatt
      901 ttcatcatcg tcggacagaa aatgccaggt cgcttgcatg cccgaaagaa gcgggttgag
      961 gctggtcatg agttgatccc ggtcaaagtt gtcatgcggc gattgaacgc atccgatgtg
     1021 caatcctgca ggatggtggt tcaacagatt cctgagcagt gtgacggatt ccggatccgg
     1081 gatggacttg ccgtccgttg cgaataatag tgttttcagt tgccgatatt ccgccgtttg
     1141 tggaatgctc aacaccggaa cagtactgct gcgcaataat tccagcgcgg tatttccaat
     1201 cagcctttcc tgcaccgcgt ttttgccttt cattcccgtt acgatcagtc cggctcctgc
     1261 ataggtagcg tagtcgttta tcgctcgccg ggtttcaccg tttctgctcg caagtgtaat
     1321 gttcaaggag gctccgacgg aagaccgcag ttcttgtgcg atctgtttga gtgcggattc
     1381 ggtatccgat ttgagttcat cgggtgttac gatcagttca atcggcaggg tttttagcgg
     1441 attgggcagg tggtaaacat tgagcagatg caaatcggtt ccggtatccc gagccaggga
     1501 ggcggcgaaa agcgcggcgt gtaaggcggc atctgaaaaa tcggtaggaa ccagaatgga
     1561 tttcatgaaa tcaggagcat ttagtaattt aatccaatgt acgttttccc tccgaagttt
     1621 cactgaccgc aatcaaggcc ctacctgact ggggtcatag agcatcccga aaaaccttaa
     1681 caaattgcat aaaatcctcc ccgatgaacc agatcctctg ccctacggat ttctcgcccg
     1741 ctgccggcca tgccgtcgct tatgcagccg cattgtccga acaaatcaac gcccgcctgg
     1801 tgctgttgca cgcgtatgaa tctcctgtcg tgttttcgag tgctcctttg acgagcattc
     1861 gtgacgcgaa gcagcagatt aagctggctg cccaaaagaa attggataaa ttggtgcaac
     1921 agctcgaaaa aatccacccc cgccttgcaa ccgaaacgaa gttgttggcc ggcttgggtt
     1981 cagagcgaat actcgatgcc gcccgcgaca tccatgccga tatgattgtg atgggcgcta
     2041 ccggcaccag taagctcagt cgtttactga tcggcagtac gaccgcagcg gttatccgcg
     2101 acgcggattg tccagtgctg tgtattccca aagaagcgaa attcaacggg atcaacagga
     2161 tcgctttcgc gaccgacctg caggaagata acctggcttc agccatgacc gtgacgccgt
     2221 tcgcggcaaa attcggcgcc gagctctcgt ttatttacgt cgatgataag cacctcttgc
     2281 attccgacga agcgatcgaa gagatgacgc gacggatccg ccggcgtgtg aagtatccga
     2341 aactctcggg cttcatcgca aagaacccca gcattacgaa aggtttagaa accttcctga
     2401 aaaagcagcc ggccgatctg ctggtgatgt tcacccatcc ccggcgtttg ggagagacgc
     2461 tttttaacca gagtgtcacc cggatgatgt cgcatcaagc caagatcccg ttgctggcca
     2521 ttcgtagaat ggatcaaccg gttccttccg gagagcggaa gcgttgaatg gatttccctt
     2581 tctgaggcaa cccgcatcgg ctgccgcttt cagtcgatgg cacgcaaggt gtatagaccc
     2641 ttcgtcaacc tgatgaatgt cattctcgaa ccggtcgcct gcccataact ttgattggta
     2701 a
//
LOCUS       DAOTON010000359         2701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29445_length_2701_cov_2.880952, whole genome shotgun sequence.
ACCESSION   DAOTON010000359 DAOTON010000000
VERSION     DAOTON010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29445_length_2701_cov_2.880952"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="P5292_07690"
     CDS             complement(<1..775)
                     /locus_tag="P5292_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HRS39048.1"
                     /translation="MKKTLTRNEHSELVEAFSEFKDLKNIDRASMMSILEDVFKNMLK
                     KKYGTADNFDIVINTDKGDLEAFRNRLIVDDGAVEDPSTQIEYSEAILIEPDFEVGEE
                     VSEKVRFTDFGRRAVLAARQNLMARVQELEKESIFQKYKNRVGEIITGEVYQVWKKEM
                     LVMDDDGNELILPKSEQIPADFFKKGDAVRAVVLRVEMINGTPKIVLSRTSPLFLEKL
                     FELEVPEVFDGLITIKKIVREPGERAKVAVESYDDRIDPV"
     gene            complement(782..1255)
                     /locus_tag="P5292_07695"
     CDS             complement(782..1255)
                     /locus_tag="P5292_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HRS39049.1"
                     /translation="MTLHDEIARLTQEKIAGSPLFLVEVKVSPNKVTVYLDHPKGVGI
                     EDCVAVSRHLQSAFDGTPVFEQHELEVSSPGMDEPLKVLPQYHKRIGRRVNVITFDGL
                     KHTGTLVSADDLGIELDREIARKVNGRKQITHESVRLPFEQIKETRVIFNFDQLR"
     gene            1406..>2701
                     /locus_tag="P5292_07700"
     CDS             1406..>2701
                     /locus_tag="P5292_07700"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRS39050.1"
                     /translation="MAEKSQIFDVFKKSPDKERVLILGGGFAGIETALRLDTRRFEVL
                     LLDRHNYFTFQPLLYQVATGGLEPDSIAYPLRQIVRRNRGVLFRVAEIESLDPIARQV
                     VTSIGTLPYDHAVIATGSMTNFFGDQQLQQHALSLKQVRDALDMRSFILQNFEEALLE
                     KDPAQRASRMNFVIVGAGPTGVEIAGALAELKRHVLPHDYPELDFADMHITLVESSDH
                     VLNGMSDKTYANARAQLEALGVQLRLGVRLSSYDGMVAKLNDGSDIRTSALLWTAGVK
                     GAVIAGIPEQGLERGNRIRVDAFNRVAGADGLYAIGDVAAMTEGGSAPHPMLGPVAMQ
                     QGKLLAKNLNRPKGQAWQPFVYKDRGTMATIGRSKAVAELAGGIRLKGFPAWMAWLFI
                     HLVLLVGFRNRVVVFFNWIWNYFSYDRAIRLIIRPYKGRT"
BASE COUNT          536 a          806 c          757 g          602 t
ORIGIN      
        1 ccaccggatc gatgcggtcg tcgtagcttt caacggccac cttggcgcgc tcgcccggct
       61 cgcgcacgat cttcttgatc gtgatgagcc cgtcgaatac ttccggtact tcgagttcga
      121 acagtttttc gaggaagagg ggagaggtgc gcgacaacac gatcttcggc gtaccgttga
      181 tcatctccac acgcaagacc acggcgcgca cggcgtcgcc cttcttgaaa aagtcggccg
      241 gaatctgttc cgacttcggc aggatcaact cgttgccgtc gtcgtccatc accagcattt
      301 cttttttcca gacctggtac acttcgccgg tgatgatctc acccacacgg ttcttgtact
      361 tctggaagat gctctctttt tcgagttcct gcacccgggc catcaggttc tgacgggcgg
      421 ccagcacggc gcgacgaccg aagtcggtga aacggacttt ttccgacact tcttcgccca
      481 cttcgaagtc gggctcgatc aggatcgctt ccgagtactc gatctgggtc gaaggatctt
      541 ccaccgcgcc gtcgtccacg atcaggcggt tacggaaagc ctcgagatct cctttgtcgg
      601 tgttgatcac gatatcgaag ttgtcggccg taccgtactt cttcttcagc atgttcttga
      661 acacgtcctc gagaatgctc atcatgctcg cgcggtcgat gttcttcaag tccttgaact
      721 cggagaaagc ttctaccagt tcagagtgct cgttgcgggt cagtgtcttt ttcatgacgc
      781 gttaacgcag ttggtcgaaa ttaaagataa ctcgtgtttc cttgatctgt tcaaacggca
      841 gacgcaccga ctcgtgcgtg atctgctttc gtccgttcac cttgcgggcg atctcgcggt
      901 caagctcgat gcccaggtcg tcggccgaca ccagcgtgcc ggtgtgcttc agaccatcga
      961 aggtgatcac gttcacccgc cggccgatcc gcttgtggta ctggggcagc actttcagcg
     1021 gctcatccat gccgggtgag ctgacttcca attcatgttg ctcgaacacc ggggtgccgt
     1081 cgaaggccga ttgcaaatgc cgggaaacgg caacgcaatc ttcgattccg actccttttg
     1141 gatggtccag gtacaccgtc actttgttcg gggaaacctt gacttccacc aggaagagcg
     1201 gactgccggc gatcttttcc tgggtcaacc gggcgatctc gtcgtgcaga gtcatgatta
     1261 tcaacaaaaa agggcccccg agggaccccc tttacgtttg gaagttgact gcaaatgtac
     1321 gcaaaatctt ctgataatga aaacaaaagc ccccggatac gctttaactt agtgcctccc
     1381 aatgtcccgc aggacacttt cctaaatggc tgaaaaaagc caaattttcg atgtttttaa
     1441 aaagtcgccc gacaaggaac gggtgctgat cctgggcggt ggtttcgcgg gcatcgaaac
     1501 ggcgctccgg ctcgataccc gaaggttcga agtcctcctg ttggaccgac acaactattt
     1561 caccttccag cctttgctct atcaggtcgc cacgggcgga ctggaacccg attcgatcgc
     1621 gtatcctttg cggcagatcg ttcgccggaa caggggtgtg ctgtttcgcg tggcggagat
     1681 cgaatccctg gatccgatcg ccaggcaagt ggtgacgtcc atcgggacgc taccctacga
     1741 ccatgctgtg atcgcgaccg gctcgatgac gaactttttc ggggatcaac aactgcaaca
     1801 gcatgccctg tcgttgaaac aggtgcgcga cgcgctcgac atgcgcagtt tcatcctgca
     1861 gaatttcgaa gaagcgctgc tcgaaaaaga ccctgcacag cgagccagtc gcatgaactt
     1921 cgtcatcgtc ggcgccggac ccaccggcgt cgagatcgca ggcgcactgg ccgaactgaa
     1981 gcggcatgtc ctgccgcacg attaccccga actcgatttt gctgacatgc acatcacgct
     2041 cgtcgagtcc tccgaccacg tgctgaacgg catgagcgac aagacctacg ccaatgcgcg
     2101 cgcgcaactg gaagccctgg gcgtgcaact ccgtctcggc gttcgcttgt cgtcctacga
     2161 tgggatggtc gcgaaactca acgacggctc cgatatccgt acgagcgcct tgctctggac
     2221 cgccggtgtc aaaggcgcgg tgatcgccgg catccccgag cagggtttgg aacgcggtaa
     2281 ccgcatccgc gtcgacgcgt tcaatcgtgt ggccggtgcg gatggtctgt acgcgatcgg
     2341 cgacgtggcg gcgatgacgg aaggcggcag tgctccgcat ccgatgctgg gtcccgtggc
     2401 catgcagcag ggaaagctgc tcgcgaaaaa tctcaaccgt ccgaaaggtc aggcctggca
     2461 gccgttcgtg tacaaagacc ggggcacgat ggccaccatc ggccggagca aggcggtcgc
     2521 ggaactggcc ggcggcattc gcctcaaagg cttcccggcc tggatggcct ggctgttcat
     2581 ccacctcgtg ctgctcgtcg gcttccgcaa ccgtgtggtg gttttcttca actggatctg
     2641 gaactacttt tcctacgacc gggccattcg gctgatcata cggccgtaca aaggccgaac
     2701 g
//
LOCUS       DAOTON010000360         2691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29577_length_2691_cov_3.161229, whole genome shotgun sequence.
ACCESSION   DAOTON010000360 DAOTON010000000
VERSION     DAOTON010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29577_length_2691_cov_3.161229"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="P5292_07705"
     CDS             complement(<1..918)
                     /locus_tag="P5292_07705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRS39051.1"
                     /translation="MERVRSLRWLFPLAVLLPILLVAVSGYDGMEFNGDAYRYYESAT
                     NLVNGWHSGGAIVTGFWPLGYPLIAALFMMIVGIGFPAAQAVSFATAFLVLLFIGKLH
                     RKEYSVVAPELFAGSALLAVSGYWLRYSTTIMSDMAAVCWSLGAIYFIRRWSDAYAWR
                     FLVVAAFFAAFSIATRYVSGLVLLPLSYCVIRYSTTARLRAIRLFGLYGCVLLFFLPQ
                     LPFSLRMPEASVQHPWLSQWSFLNFLASDRTTIDGHLQVSWPNLLYYTLAPFRWSELT
                     PVGGLLALVGCWQRFRHGFSRADAMMGIWY"
     gene            complement(1008..>2691)
                     /locus_tag="P5292_07710"
     CDS             complement(1008..>2691)
                     /locus_tag="P5292_07710"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39052.1"
                     /translation="NGCTRTASVAVSSSGSAPATPGAISGPSGGCRGQNGVVFSVPAV
                     AGATSYQWTLASGMTGTSTGNSITVNFTNSFSGGSICVRAVNVCGNSQYSCKSLTRFT
                     AAPANAGTISGPATICGPLTATYSVAPVANATSYNWTLLAPGATIASGQGTNSITVNF
                     NSNYVVGILAVQASNCIGSGSYSFKLILGQLVLAPVFTATNNPTVGVCGGSTKTYEIF
                     KFPNATSYTWSAPAGSVITSSLGQSGNPLTVDSTVGKVSIKFPSGFSSGTVSVYATNA
                     CGNSPVASLSVRSTPLAPATISGPTAGVCRLSNQTYSISAVTGATSYTWTVPSGATIT
                     NNSGTSIRVTFGNSFTGSGNITVKANNACGSSAVTSLLVKAAPAQPSGISGPATVCKS
                     QTSVSYSVPAVANTSSYTWTISGGAAFVGSTSGRTVTVRFNGATSTTVTLTARANNTC
                     GSSTTASMNVTVNFGCRTVGDELIETDEPILAYPNPTRGVLHLNFLSTTEQRQHLMVT
                     DLTGRVLQEQDVQVKEGENFLELDLTGYARGAYLVTFLREDAAAKTLRILVE"
BASE COUNT          667 a          779 c          750 g          495 t
ORIGIN      
        1 gtaccagatc cccatcatcg catcggctct cgaaaatccg tgtcgaaacc gctgccagca
       61 gccaacgagg gcaagcagcc cgccgacagg cgtcagttcc gaccagcgaa acggtgcgag
      121 ggtataatac aacaggttcg gccagctcac ctgcagatgt ccgtcgatcg tggttcgatc
      181 agaggcaaga aaattcagga acgaccactg tgacaaccag ggatgttgca cggaagcttc
      241 cggcatgcgc aaactgaatg gcaactgcgg taaaaaaaac aacagcacac atccatataa
      301 gccgaacaac cggattgcac gcaaccgtgc agtcgttgag tatcggatca cacaatatga
      361 taacggcaat aatacgagtc cgcttacata acgcgtggcg atcgagaatg ctgcgaagaa
      421 ggctgcgaca acgagaaagc gccaggcgta cgcatcactc caacgacgta tgaaataaat
      481 cgcgccaaga ctccaacaca cggccgccat atccgacatg atcgtggtgg aatagcgcag
      541 ccaataaccg ctgacggcca gcaaggcgct gcctgcaaaa agctccggcg ctacaacgga
      601 atactccttg cggtgaagtt tcccgataaa cagtagcacc aggaaagccg tcgcaaaact
      661 gaccgcctgg gcggccggaa agccgatacc gacgatcatc atgaataaag cggcaatcaa
      721 cggatagccg agcggccaga atcccgtaac aattgctccg ccggaatgcc agccatttac
      781 caggttcgtc gcgctctcgt aataccgata cgcatccccg ttgaattcca tgccgtcgta
      841 cccgctcacg gctacaagta ggatcggcaa cagcaccgcc aacggaaata accagcgtaa
      901 ggatcgcacg cgctccattc ggattcaaga tagcactacg tttccatagc ccgcaataaa
      961 aaacggggcc ggaaattttc cggccccgtt ccatggagta caatggatta ctcaaccagg
     1021 attcgcagcg ttttcgctgc tgcatcttcg cggaggaagg tgaccaggta agcgccgcgc
     1081 gcatagcccg tcaggtccag ctccaggaag ttctcgccct ccttcacctg tacatcctgc
     1141 tcctgcaaga cacgtccggt caggtcggtg accatcaggt gctgtcgttg ctcggtggtg
     1201 gacaagaaat tcaaatgcag gacaccgcgt gtcgggttcg ggtaagccag gatcggctca
     1261 tccgtctcga tcagctcgtc accaacagtc cggcaaccga aattgacggt cacattcatc
     1321 gaagcggtgg tcgagctgcc acaggtgttg ttcgcacgcg cggtgagcgt aacggtcgtg
     1381 ctggtagcac cgttgaaccg tacggtgacc gtccggccgc tcgtactacc aacgaatgcc
     1441 gcgccaccgg agatcgtcca ggtatacgat gaagtgttgg ccacggcagg caccgaataa
     1501 ctcaccgagg tctgtgattt gcaaacggtg gcaggcccgc tgatgcccga aggttgagca
     1561 ggagcggctt tcaccaacag cgaggttacg gcactgctgc cacaagcgtt gttcgctttc
     1621 acggtaatat tacccgatcc agtaaagctg ttaccaaagg tgacacgaat cgatgttccg
     1681 ctgttgttgg taatcgtagc gccggaaggt accgtccagg tataagaggt ggcgccggtc
     1741 actgcgctga tcgaataggt ctgattgctc aaacggcaga cccctgccgt agggccgctg
     1801 atcgtggccg gagccaaggg cgtggaccgc acggaaaggc tcgcaaccgg actattacca
     1861 caagcgttcg tggcgtaaac gctgaccgtg ccgctgctga agccgctcgg gaatttgatc
     1921 gagaccttgc ccacggtact gtcgacggtc agcggattac cgctctgtcc gagactgctg
     1981 gtgatcacac tgccagccgg agcgctccag gtgtaggagg tcgcgttcgg gaacttgaag
     2041 atctcgtagg tcttcgtcga accgccgcac acgccgaccg tcgggttatt agtagctgtg
     2101 aagaccggcg caagcaccaa ctgacccagg atcagcttga agctgtacga accgctgccg
     2161 atgcaattgc ttgcctgtac agccaggata ccaactacat aattcgagtt gaagttgacc
     2221 gtgatcgaat tggttccctg accggaagcg atcgtggcac caggcgcaag cagcgtccag
     2281 ttgtaggagg tggcattcgc aacaggcgcc acgctgtaag tagccgtcaa cggaccacag
     2341 atcgtagccg gaccactgat ggttcctgca ttggcgggag ccgcagtaaa gcgtgtcaag
     2401 gacttgcagg aatattgact gttaccgcaa acgttcactg cacgaacaca gatcgaaccg
     2461 ccactgaagc tattggtgaa gttcaccgtg atcgaattgc cggtcgatgt accggtcata
     2521 cccgatgcaa gcgtccactg ataggaagtc gcaccggcaa cggcaggaac gctgaacaca
     2581 acgccgttct ggccgcgaca accgcccgaa ggtccgctga tcgcacccgg agtagccggt
     2641 gctgaaccgc tgctcgaaac agccactgat gccgtacggg tacagccgtt c
//
LOCUS       DAOTON010000361         2689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29611_length_2689_cov_3.079727, whole genome shotgun sequence.
ACCESSION   DAOTON010000361 DAOTON010000000
VERSION     DAOTON010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29611_length_2689_cov_3.079727"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..649)
                     /locus_tag="P5292_07715"
     CDS             complement(191..649)
                     /locus_tag="P5292_07715"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRS39053.1"
                     /translation="MAKRKAKPESFIRKPVFPGGNTAFQEFIRTNLRYPEEAVANRIE
                     GAVKVAYDVDVFGKVIEAKVTHGIGYGCDEEALRLVRLMQYPKKKYQGMHVVFHMVVT
                     IHFRLNPVSKPDTGASIKLNYVGDGKTTTPEKKNGGATYTYSIRIDPDKN"
     gene            complement(736..1296)
                     /gene="efp"
                     /locus_tag="P5292_07720"
     CDS             complement(736..1296)
                     /gene="efp"
                     /locus_tag="P5292_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRS39054.1"
                     /translation="MADTSDIKVGSILRYNGELCQVTEYIHRTPGNLRAFYQVKMRNL
                     KSGKVVENRFRAGEQVELARVEFKEMQYLYPEGENMVVMDPETYDQVYISKSMFGENA
                     DLLKEEMIVKVAFEGDNAITAEAPTFVELAITYTEPGVRGDTATNTLKPAKVETGATI
                     MVPLFVNDGEVIKIDTRDRSYVGKVK"
     gene            1609..2337
                     /locus_tag="P5292_07725"
     CDS             1609..2337
                     /locus_tag="P5292_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39055.1"
                     /translation="MRVILCALWAILQVSFCANGQTDSTRVLVRGRAFDAADPNRRLD
                     DLMIINLRTNQGTFGLADGSFQSVVERADTFIIASTGYEFRRICLADSAGRAEYFLRV
                     PLTRLNVQLKEVAIFSPRQLDAIYRDIEKLGYRKRDFQIGGVDALQSPITFLYQEFSR
                     LEQLKRRNAERINNERRRELLRELLANYAAADIIHLDNSEFDDFIDFANIPEQYMKTA
                     TQYDFCIHVKRKFELYRMTRGRKF"
     gene            complement(2411..>2689)
                     /locus_tag="P5292_07730"
     CDS             complement(2411..>2689)
                     /locus_tag="P5292_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HRS39056.1"
                     /translation="GIELSTSQQVTMMLMLMITSKGMAGVPRASLVVIAGMLHAFNIP
                     QEGLTLLLAIDWLLDMGRSATNVMGNAVATAVVSKWEGYSVGHGHAEE"
BASE COUNT          580 a          688 c          732 g          689 t
ORIGIN      
        1 tacattataa atcgtaacgg aatccggaaa atttcctgga taaccagggt gagtccagtc
       61 ggaaaggcgt tcgggcggaa gtgtctgtgc ggtggcattc agccaaccgg taatgcagag
      121 cagatagagg agggggcgca tgcgttgtca agttagcgaa cttttccaac gaagacacat
      181 gcgtcagcac tcagttttta tccgggtcga tgcgaatgga gtaggtgtag gtggcgccgc
      241 cgttcttttt ttccggcgtt gtggttttgc catcaccgac gtaattcaat ttgatcgacg
      301 cacctgtatc aggcttgctg accgggttca gccggaaatg aatcgtgacg accatgtgga
      361 agacgacgtg cataccctgg tatttcttct tggggtattg catcagccgc acgaggcgta
      421 gcgcttcttc atcgcagccg tacccgatac cgtgcgtgac cttcgcttca atcacctttc
      481 cgaagacatc gacatcatac gcgactttga ccgctccttc tatccggttg gccacggcct
      541 cttccggata acggaggttg gtacggatga actcctgaaa agccgtgttc cctcccggga
      601 aaacgggttt acgaataaaa ctttccggtt tagccttccg ctttgccatg ttaaaggata
      661 cgcaatttca gtcgggaggt tcctataaaa agaaaggcca tccggaggga tggccttgca
      721 tgaagtcgta attcgtcatt tcaccttgcc gacgtagctg cgatcgcggg tgtcgatctt
      781 gatgacttcc ccatcgttta caaacagcgg aaccatgatg gtggcgccgg tctccacctt
      841 ggcgggcttg agtgtgttgg tagcggtatc gccacgaaca cccggttcgg tataggtaat
      901 ggccagttcg acaaaggtcg gggcttctgc ggtaatcgcg ttgtcgcctt cgaaagcaac
      961 cttgacgatc atctcttcct tgaggaggtc ggcgttttcg ccgaacatgg atttggagat
     1021 gtacacctga tcatacgttt ccggatccat cacgaccatg ttttcgcctt ccggatagag
     1081 gtactgcatt tccttgaatt ccacgcgggc cagttcaacc tgttcgcctg cacggaagcg
     1141 gttttcgacc actttgccgg atttgaggtt gcgcattttt acctggtaaa aagcgcgcag
     1201 gttgccgggg gtgcggtgga tgtattcggt cacctggcag agttcgccgt tgtaacgaag
     1261 gatgctgcct actttaatat cggatgtatc tgccatgggc tgattttatt gattttcaag
     1321 ggttgcaaag gtagtgaatc ccggccgatt ttgaaagaaa tgccggattc cgtgaggatt
     1381 tcaaacattt taggacctgc cagcgttcct atcctgctaa cagtacatgg tattttgcct
     1441 gggtaccact aaattgattc cgtttatcag ctgttttgaa acagctgatt gcatggtaat
     1501 tgacagggcc ggagcctgct gtgccccagc catgttaaca tactttatca catcaaactc
     1561 aaccgtcaga tccacttgag cctgacgaat caactacctt cgcccgacgt gcgagtcatt
     1621 ctttgtgccc tctgggccat tcttcaggtt tctttctgcg ccaatggaca aaccgatagc
     1681 acccgtgttc tggtgcgcgg ccgggcattc gatgcagccg atcccaaccg gcgtctggat
     1741 gacctgatga tcatcaacct gcgcaccaat cagggtacgt tcgggttggc ggacggcagt
     1801 tttcaatcgg tggtcgagcg tgccgatacg ttcatcatcg ccagtaccgg ttatgagttc
     1861 cggaggattt gtctggcgga ttccgcaggt cgcgctgagt acttcctgcg tgtgccgctg
     1921 accaggttga acgtacagct caaagaggtt gctatctttt ctccccgaca gctcgatgcc
     1981 atttaccgcg acatcgaaaa attgggatat cgtaagcgtg atttccagat cggaggcgtg
     2041 gatgcgctcc aaagtccgat cacgttcctg tatcaggagt tcagccggtt ggagcaattg
     2101 aagcggcgaa acgcggagcg gatcaacaac gagcgccgac gcgaactgtt gcgtgagttg
     2161 ttggcgaatt acgccgcggc tgatatcatc cacctcgaca attcggagtt tgacgacttt
     2221 atcgacttcg cgaacattcc ggagcaatac atgaaaacgg ctactcagta cgatttctgt
     2281 attcacgtca aacggaaatt cgagttgtac cggatgacgc gcggcaggaa gttctgatcg
     2341 attcacagcg tgtgggtgtt agaaaccgtg ccgagtgatc agttggaccg ttggtacttt
     2401 ttcaatctgt tcattcttcg gcatgaccgt ggcctaccga atagccttcc cacttactga
     2461 ccacagcggt cgctaccgcg ttccccatca cattcgtggc ggaacgtccc atgtcgagta
     2521 gccagtcgat cgccaacaaa agggtcaatc cttcctgggg gatgttaaac gcgtggagca
     2581 tgccggcgat gacgaccagc gacgcacggg gcacgccggc catgcccttg ctggtgatca
     2641 tgagcatgag catcatggtc acctgttgcg aggtgctgag ttcgatccc
//
LOCUS       DAOTON010000362         2678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29741_length_2678_cov_3.857034, whole genome shotgun sequence.
ACCESSION   DAOTON010000362 DAOTON010000000
VERSION     DAOTON010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29741_length_2678_cov_3.857034"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="P5292_07735"
     CDS             <1..588
                     /locus_tag="P5292_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39057.1"
                     /translation="RASEFRDGWKKQFEHVRFRTYDYAKLGADKLQDSLSKGKSNVIF
                     LPSSNEDLVTAVLNKTKELKADYELKIVGLPTWMHFETIDAALMESAGVELFTSAYLQ
                     QDIPDQAGMRKSFRDRYAAEPDENALLGADAIDILLNGWKKSGADFPDELSLPNYTGR
                     YCDYRFSKTEAGSCMENMNIRIWEFRDRKPIEVGQ"
     gene            603..1508
                     /gene="hemF"
                     /locus_tag="P5292_07740"
     CDS             603..1508
                     /gene="hemF"
                     /locus_tag="P5292_07740"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HRS39058.1"
                     /translation="MATDRRYIADWLMSLQDRITEGLEVADGTGRFREDNWERPGGGG
                     GRSRVLTNGSVIEKGGVNFSAVFGSTPDFLKQQLGPEAGSEFFATGVSIVIHPNSPQV
                     PIIHMNVRYFETGSKVRWVGGGIDLTPHYVKREDARFFHQTLKATCDRFDEAYYPAFK
                     QWADDYFFIKHRKETRGVGGIFFDHLKADDRHGFDDRIRFMQAVGDTFLPTYTELIRR
                     NAGIAFTEREKQWQLIRRGRYAEFNLVYDRGTKFGLETDGRIESILMSLPPLASWEYD
                     FQPAAGTPEAETQALLIKGIDWIKG"
     gene            1520..2182
                     /locus_tag="P5292_07745"
     CDS             1520..2182
                     /locus_tag="P5292_07745"
                     /inference="COORDINATES: protein motif:HMM:NF027590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4918 family protein"
                     /protein_id="HRS39059.1"
                     /translation="MLSERIIRFYFSLPSRLHVPNGVEVLSVYQDPEVRGFVEAFYSR
                     YYHDERPRTLVLGINPGRLGGGMTGIPFTDPVRLSEDCGIANQLEKRTELSSDFIYRM
                     IRHLGGPAKFFGKNLLSAVCPLGFTRAGKNINYYDDPELLDATRPFILDTLQKQFALG
                     VNLDTVILLGEGQNAKHFLQWNRQYGFCRQVVSLPHPRFIMQYKRKALPEYLDRYAAV
                     LA"
     gene            2281..2421
                     /locus_tag="P5292_07750"
     CDS             2281..2421
                     /locus_tag="P5292_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39060.1"
                     /translation="MSHDHHAEEPKRGIWYTYLGETGAAVVAFIWILLTLSWIFGVVN
                     FG"
     gene            complement(2488..>2678)
                     /locus_tag="P5292_07755"
     CDS             complement(2488..>2678)
                     /locus_tag="P5292_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39061.1"
                     /translation="YRSANHYAVYENWVDCVADYKLWQEHTFRLTDRYLDFLGTYYAE
                     DHQYADKIRNMVDRRKGS"
BASE COUNT          659 a          660 c          709 g          650 t
ORIGIN      
        1 cgtgcttctg aatttcggga tggatggaag aaacagttcg agcatgtccg ttttcgcacg
       61 tacgattacg caaaactggg cgctgacaag ttgcaggact ccttgtccaa gggcaagtca
      121 aatgtgatct tccttccttc cagcaatgag gatcttgtca ccgccgtgtt gaacaagacc
      181 aaggaactga aagccgatta cgaacttaag atcgtgggtt tgccaacctg gatgcacttt
      241 gaaaccattg acgccgcgct tatggaatcg gcaggtgttg aactgttcac ctcggcatac
      301 cttcagcagg atatcccgga tcaagccgga atgcgaaaat cgtttcgcga tcgctatgcc
      361 gcagaacctg atgaaaacgc gctgcttgga gcggatgcca tcgacattct tctaaacggc
      421 tggaaaaaat caggagcgga ttttccggac gaattatcac tcccgaacta cactggacgt
      481 tattgtgatt atcggttcag caagactgaa gccggctcct gcatggagaa catgaacatc
      541 cggatatggg agtttcgtga ccgaaaaccg atcgaggtag gccagtaatg gtcgagttga
      601 ctatggctac tgaccgccgg tatattgccg attggctaat gtcccttcag gatcgcatta
      661 ccgaaggact tgaagtcgct gacgggaccg ggcgtttccg ggaagataat tgggaacgac
      721 caggcggcgg tggcggaaga tcccgggtgc tcaccaacgg ttcggtaatc gaaaagggcg
      781 gggtcaactt ttcagcggtt ttcgggagta ctcctgactt tcttaaacag cagttgggcc
      841 cggaggcagg ttccgagttc ttcgcaaccg gtgtttccat tgtcatccat ccgaattcac
      901 cccaagtgcc gatcatccac atgaatgtga ggtacttcga aacaggctca aaggtgcgct
      961 gggtcggtgg cgggattgac ctgaccccac attatgttaa acgggaggac gcccggttct
     1021 ttcatcagac tttgaaagca acctgtgatc gctttgatga agcctattat cctgcattca
     1081 aacaatgggc cgacgattac ttcttcatta aacaccgcaa ggagacaaga ggcgttggcg
     1141 ggatcttctt tgaccatcta aaggcggatg atcgacacgg gttcgatgac cggatccggt
     1201 tcatgcaagc ggttggagat acttttcttc cgacctacac ggaactgatc agaaggaatg
     1261 caggaattgc gttcaccgaa cgcgaaaagc aatggcaatt gatccgccgc ggacgctatg
     1321 cggagttcaa cctggtgtac gatcggggaa cgaaattcgg gctcgaaacc gacgggagga
     1381 tcgaatcgat cctgatgagt ttaccgcccc tggcgtcctg ggaatacgac tttcaaccag
     1441 ccgccggaac tccggaagcg gaaacccagg cacttctgat aaaaggaatt gattggatca
     1501 aaggttgatt ggttcaacga tgctctccga acggatcatc cggttttatt tttcactgcc
     1561 gtcgcgttta cacgtgccga atggagtcga agtactttcc gtttatcagg acccggaggt
     1621 tcgtggattc gttgaggcgt tttactcgcg ttactatcac gacgagcgac cgcgcacact
     1681 tgttctgggt atcaatcctg gccgtctggg tggcgggatg acgggcattc cgttcaccga
     1741 tcccgtacgg ctgtcagaag attgtggtat cgcgaatcaa ctggagaaac ggactgaact
     1801 cagcagcgat ttcatctacc ggatgatccg gcacctgggt ggaccagcaa agttctttgg
     1861 aaaaaatctc ttaagcgctg tatgtccgct ggggttcacc agggctggta agaacatcaa
     1921 ctattatgac gatccggaat tgctggatgc gacaaggcct ttcatactcg ataccctgca
     1981 aaaacaattc gcgctcggcg ttaatctgga cacggtgata cttcttggcg aaggccaaaa
     2041 tgcgaaacac ttcctgcaat ggaatcgtca gtacggcttt tgtcggcagg tggtcagtct
     2101 tccgcatccg aggttcatca tgcaatacaa gcgaaaagcg cttccggagt atctggaccg
     2161 ttatgccgct gttttggcct agggagaagc acgttaataa ttcgccgccc ggcaacccgg
     2221 gaaggctttt gtccgtttta gtgagaatca cgatatttga cgctccataa agaaaaacct
     2281 atgtcgcacg atcatcacgc agaagaaccc aaacgcggaa tctggtacac gtaccttgga
     2341 gaaaccggcg cagccgttgt cgcctttatc tggatcctgc tgaccttaag ctggatcttc
     2401 ggagttgtca atttcggttg atctcgaccc agacaaatac aaagcccggc catagtgccg
     2461 ggcttttttg attttagtta ttaccgttca ggaccctttt cgacggtcta ccatgtttcg
     2521 gatcttatca gcgtattgat ggtcttccgc gtaataggta cccagaaaat caaggtaacg
     2581 gtccgtcagg cgaaaggtat gttcctgcca caacttgtaa tcggccacac aatcgaccca
     2641 gttctcatag actgcataat ggttggcact tcgatact
//
LOCUS       DAOTON010000363         2677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29765_length_2677_cov_2.638444, whole genome shotgun sequence.
ACCESSION   DAOTON010000363 DAOTON010000000
VERSION     DAOTON010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29765_length_2677_cov_2.638444"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="P5292_07760"
     CDS             <1..1134
                     /locus_tag="P5292_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39062.1"
                     /translation="SDGADYAAHMTLISSAADKQTPPLGTLTRDVLTPEIFSRMQATG
                     DTICPYVDTRHPSGIPDSGIVSFLETPRFLSGWAALHHCIGFITESHMLKPFPERVNS
                     THRILQAILKTAAKNQAAIAHARRSALLHYRHGERFPLNWILDTTRVGTLDFSGYKAR
                     YRTSKVTGLDRLYYDHDAPYRKPVPFYDHYTGIDSTSIPRYYILPQAWNKVAELLLLN
                     NVKMKRLSQDSSLDVSAYRITNYNTVDRPYEGHYLHYQVSTSVAREKVQCFRGDWIIS
                     TDQPAARYVFEALEPTATDSYFNWGFFDAVLQQKEWFSDYVFEDVAAELLESQPALKR
                     DFLEWRSKHPELNGDAFAQLLYIYRHSPNFETGSMRYPVYRID"
     gene            complement(1186..1539)
                     /locus_tag="P5292_07765"
     CDS             complement(1186..1539)
                     /locus_tag="P5292_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39063.1"
                     /translation="MRQGRSKQNTNTTREQAPLTIVKTGESQAHLEREIDQHINNIRA
                     LIRQLRPSERQRYYNGLLSHILSEPVEPSFHPTRIDRQEEQSTNLSKEHLELIREMAV
                     KMEQMYRVMDGYDPS"
     gene            complement(1610..>2677)
                     /locus_tag="P5292_07770"
     CDS             complement(1610..>2677)
                     /locus_tag="P5292_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS39064.1"
                     /translation="GGVLNTGTIYMCVIIMTAFMLLGTKAGKWLAALAVASIIFLFYA
                     TEYTSMTSYALFNNDSSLIHQDALTTFILGVFLVAAQSNNLHSGKNVIIERITDQKNE
                     LERQNQKLEEYTHHLEKTNKELDKFASIVSHDLKAPLRAIGNLTGWIEEDAGDSLVPE
                     VRANFNMIKQRVRRMEDLINAILDYSRADRVKGDEELIDTQALIKDSFDFIGSPENSE
                     LVIKGEMPRFVSDKTRLVQVFSNLLGNAIKYNDKPEIKIEVGCEEQSNGWIFSIKDNG
                     PGIEPQYHEKIFVIFQTLNRRDDVESTGVGLAIVKKIIEDQGGRIWVESELGKGADFR
                     FFWPKVPKQKDAALIAAAMVV"
BASE COUNT          598 a          717 c          660 g          702 t
ORIGIN      
        1 agcgacggcg ccgattacgc ggcgcacatg accctgatca gcagtgctgc cgataagcaa
       61 acgccgccgc tcggtacgct tacgcgtgat gtccttacac cggagatctt ttcccgaatg
      121 caggccaccg gcgataccat ttgcccctat gtcgacaccc gtcacccttc gggcattccc
      181 gacagcggta tcgtttcgtt cctggaaacg ccgcgcttcc tgagtggatg ggcagccctg
      241 caccattgca tcggattcat caccgaatcg catatgttga agcctttccc ggaacgggtt
      301 aattcaaccc accggatcct ccaggccatt ctgaaaactg cggcaaaaaa ccaagcggcg
      361 atcgctcatg cgcgaaggag cgcccttctc cactatcgcc acggcgaaag gtttccgctc
      421 aactggattt tggacactac ccgagtcggg acactggact tttctggata caaagcccgc
      481 taccgtacaa gcaaagtaac cggactcgat cggttatatt atgaccatga tgctccctac
      541 cggaaaccgg ttccgtttta tgatcactat acaggcatcg actcgacctc cattccccgt
      601 tattacatct tgccacaggc ctggaacaag gtcgcggaac tgctattgct gaataacgtg
      661 aaaatgaaaa ggctatcgca agactccagt ctcgacgtca gcgcataccg gatcaccaac
      721 tacaacacgg tcgatcgtcc gtacgaggga cattatttgc attatcaggt atcgacaagc
      781 gttgctcggg agaaagttca atgttttcgt ggcgattgga tcatatcgac tgatcaaccg
      841 gccgcacggt atgttttcga ggcgttggag ccaaccgcta ccgattctta tttcaattgg
      901 ggtttctttg atgccgtact gcaacaaaag gaatggttct ccgattacgt atttgaagat
      961 gtcgcagcag aactactgga atcacaaccg gcgctgaagc gggattttct cgaatggagg
     1021 tcgaagcacc ccgaattgaa tggggacgcc ttcgctcagt tactgtacat ctatcggcat
     1081 agtcctaatt tcgagaccgg ctccatgcgt tatccggttt accggataga ctaaaaaaaa
     1141 gagccgtcct ttcggacggc cctcgtctaa ccaaaaacca gactatcagc tgggatcgta
     1201 accatccatg acccggtaca tctgttccat cttcaccgcc atttcgcgga tgagttcgag
     1261 atgttccttg gaaaggttgg tagattgttc ctcctgtcgg tcgatgcgcg tcggatgaaa
     1321 ggacggctca accggttcgg agaggatgtg cgataacaat ccgttgtagt agcgttgacg
     1381 ctccgatggt cgtaactgtc ggatcagcgc acggatattg ttgatatgtt gatcgatctc
     1441 gcgctcgagg tgcgcctggg attcaccggt cttgacgatg gtcaacggtg cttgttcccg
     1501 ggtcgtattg gtgttttgtt tgcttcttcc ttgtcgcatg ttcttccgtt cgtttcatac
     1561 aattttacac gaaacggaag gggaagttga agcaattcgc cggcgtatct tacacgacca
     1621 tggccgcagc gatcagtgcc gcgtcctttt gttttgggac tttaggccag aaaaaacgga
     1681 aatccgcccc cttgccgagc tcggattcca cccaaatcct gccaccctgg tcttcaatga
     1741 tcttcttgac gatggccaat ccgacgccgg tgctttccac gtcatcgcgg cggttcagcg
     1801 tttggaagat cacgaaaatc ttctcgtggt attgcggttc gataccggga ccattgtctt
     1861 tgatggaaaa gatccaacca ttggattgct cctcacatcc gacttcgatc ttgatctccg
     1921 gcttgtcgtt gtatttgatc gcgttgccca gcagattgga gaacacctgt accaatctcg
     1981 tcttgtcgct cacaaatcgt ggcatttcac ctttgatgac cagctccgaa ttctccggac
     2041 ttccgatgaa atcgaaactg tccttaatca atgcctgcgt atcaatgagc tcctcatccc
     2101 ctttcacgcg atcggctctt gaatagtcca gaatggcatt gatgagatcc tccatcctgc
     2161 gcacacgttg tttgatcatg ttgaaattgg cacggacctc cggaacaaga ctgtctccgg
     2221 catcttcttc gatccaaccc gtcaggtttc cgatggcccg aagcggggct ttcagatcgt
     2281 gcgaaacaat ggaagcgaat ttgtccagtt ccttgttcgt tttttccagg tgatgggtgt
     2341 actcttccaa tttctggttt tgcctctcca gttcattctt ttgatccgta atccgttcaa
     2401 tgatcacgtt ttttccacta tgcaggttgt tgctttgagc ggcaaccagg aatacgccga
     2461 gaatgaaggt tgtcaacgcg tcttgatgta tgagggacga atcgttgttg aacaatgcgt
     2521 aactcgtcat ggacgtgtac tccgtggcgt agaataggaa gatgatactg gccaccgcga
     2581 gtgccgcgag ccatttgccg gcttttgtgc cgagtaacat gaaggccgtc atgatgatga
     2641 cgcacatgta gatcgttccg gtattgagta ctccgcc
//
LOCUS       DAOTON010000364         2675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29788_length_2675_cov_3.676718, whole genome shotgun sequence.
ACCESSION   DAOTON010000364 DAOTON010000000
VERSION     DAOTON010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29788_length_2675_cov_3.676718"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="P5292_07775"
     CDS             <1..553
                     /locus_tag="P5292_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS39065.1"
                     /translation="ARTDSLQTIFLNCTELADSLHIDLEWTAYHGWPATYEVVQIDSS
                     GVPFVIAGSSTTDTVYRYEKPDRNGTYQLRIRTANSGQPVLVSWSNSCRFEVRNTAVV
                     VSTVMTPNNDGRNDNFFVRNLEQYPGSRLRIFDRWGKTVYESGDYRNDWDGGKLQGDT
                     YFYILQVADNRSTEKRGTLTLLR"
     gene            565..1521
                     /gene="metF"
                     /locus_tag="P5292_07780"
     CDS             565..1521
                     /gene="metF"
                     /locus_tag="P5292_07780"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRS39066.1"
                     /translation="MKVTEHIARANGKTLFSVEILPPLKGKSIQSIYNILDPIMEFKP
                     AFIDVTYHREEYVYKKRENGLLEKFSMRKRPGTVAICSAIINHYNVDTVPHIICGGFS
                     REETENALIDLHFLNIDNVLLLRGDAIKSEPVFEAHPLGHRYAIDLVKQVANMNTGTY
                     LDEELQDVTPTNFCIGVAAYPEKHFEAPNLTTDMRWLKAKIDAGAEYLVTQMFFDNSK
                     YFAFVEKCKEMGINVPIIPGLKPITTRKQLTSLPKAFHVDIPQELIDRIEACKTDADV
                     KSEGIQWCVEQSKELMKAGAPCLHYYTMSNAEITCRIAEKVF"
     gene            complement(1696..2286)
                     /locus_tag="P5292_07785"
     CDS             complement(1696..2286)
                     /locus_tag="P5292_07785"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRS39067.1"
                     /translation="MFTGIIESTAPLVATRTEGSNLHLRFRTPIARELKVDQSVAHNG
                     VCLTVTKVEGEEYEVTAVEETLARTNLGELQQADLVNLERCMRLGDRLDGHIVQGHVD
                     TCGKVERIDSRDGSWNVYFTYDPSNGHPTVPKGSVCVNGVSLTVVESGAGRFSVTIIP
                     YTWEHTNFHRLKPGSTVNLEFDIIGKYVERLLSERK"
     gene            complement(2492..>2675)
                     /locus_tag="P5292_07790"
     CDS             complement(2492..>2675)
                     /locus_tag="P5292_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS39068.1"
                     /translation="WGQLVFETTDKDINWDGTHKDNGSDCPDGVYYYTCKVNFFRISG
                     EESVELKGYVHLIRGQ"
BASE COUNT          651 a          719 c          716 g          589 t
ORIGIN      
        1 ggcccgaacc gacagcctgc aaacgatctt cctcaactgc acggaactgg cagactcgct
       61 gcacatcgat ctcgagtgga cagcctatca cggttggccg gcgacctatg aagtggtaca
      121 aatcgacagc agcggggtgc cgttcgtcat cgccggcagt tccacgacgg acacggtcta
      181 tcggtacgaa aaaccggacc gtaacggcac ctaccagctc aggatccgga cagccaacag
      241 cggccagccg gtactggtaa gttggtcgaa cagttgccga tttgaggtcc gcaatacggc
      301 ggtagtggtc agcaccgtca tgactccgaa caacgacggc aggaatgaca acttcttcgt
      361 tcgcaacctg gagcaatatc ccggcagccg gctgcggata ttcgatcgct gggggaaaac
      421 cgtgtatgaa agcggggact atcggaacga ttgggatggc ggtaagctgc agggagatac
      481 gtacttttac atccttcagg tcgccgataa ccggtcgacc gaaaaacgcg gcaccctgac
      541 cctgctgcgt tgactgaacg atttatgaaa gttaccgaac acattgcccg cgccaacggg
      601 aaaaccctgt tttccgtcga gatcctgcct ccgctaaaag gcaagagtat ccagagcatc
      661 tacaacatcc tggatccgat catggagttc aagcccgcct tcatcgatgt gacctatcac
      721 cgggaagagt atgtctataa gaagcgcgag aatggcttgc tggaaaagtt ctcgatgcgc
      781 aagcgtccgg gaacggttgc catctgctcg gccatcatca accattataa cgtcgacacg
      841 gtcccgcaca tcatctgcgg aggtttttcc agggaagaaa ccgagaacgc gctcatcgac
      901 ctgcacttct tgaatatcga caacgtcttg ctgctacgcg gcgacgcgat caagtcggag
      961 ccggtatttg aagcgcatcc gctcgggcat cggtacgcca tcgacctcgt caaacaggta
     1021 gccaacatga acaccggcac gtacctcgac gaagagttgc aagacgttac gccaacgaac
     1081 ttctgtatcg gcgtggccgc ctatccggaa aaacacttcg aagcgccgaa cctcacgacc
     1141 gacatgcgct ggctgaaggc gaaaatcgat gcgggcgctg aatacctcgt gacccaaatg
     1201 ttcttcgaca attcgaagta cttcgccttt gttgaaaaat gcaaagagat gggaattaac
     1261 gtcccgatca tcccggggct gaagccgatc acaacgcgta agcaactcac ctctttaccg
     1321 aaagcctttc acgtcgacat tccccaggaa ctcattgacc ggatcgaggc ttgtaagacc
     1381 gacgcggatg tcaaatccga aggtatacag tggtgcgtcg aacagtcaaa agagttgatg
     1441 aaagcagggg ctccctgttt gcactactat acgatgagta acgcggagat cacctgccga
     1501 atcgcggaga aggtgttttg agggtgcgaa tagtgaatag tgaatagtga atagtgaaca
     1561 gtgaatttta cctgttgaag cttttgcgta gacagaagaa ttggaacatt cacaatagat
     1621 agatgcgcat tttgcgaaaa tgaactgtcc gccgtggctg cgctaaggag ggtatttgaa
     1681 tcaaacgctt ccggtttatt ttctttccga cagaagtcgc tccacatact tcccaatgat
     1741 atcgaactcg aggttgacag tcgagcctgg tttaagtcgg tggaagttgg tgtgttccca
     1801 ggtataggga atgatggtga cggaaaaacg accggctcct gattcgacca cggtcaggct
     1861 gacgccgttc acacaaacag aacctttcgg tacggtggga tgcccgtttg acggatcata
     1921 cgtaaagtaa acattccaac tgccgtcgcg ggaatcgatg cgctccacct taccgcacgt
     1981 atccacatgc ccttgcacga tgtgaccgtc gagacggtcg ccgaggcgca tgcagcgttc
     2041 caggttgacc aggtcggctt gctgtagctc gcccagattg gtacgcgcca atgtttcctc
     2101 taccgccgtc acttcgtatt cctccccttc caccttcgta accgtcaggc agaccccatt
     2161 gtgcgccaca ctttgatcca ccttgagctc gcgagcaatc ggcgtgcgaa aacgcagatg
     2221 caggttagag ccttcggtgc gtgtcgcgac cagtggggcg gtggattcga tgatgccggt
     2281 gaacatacgc aggttctggg tttcagattc cgggtttcct gccttcacta aagctacggc
     2341 aggcaggcgg gttccgagtt gaaagatgaa agttggaagt tagcttggtc tcaattgcat
     2401 ttcggattcg ccgacctaaa caattcaatg ttctggaagc acttcggaca atccgtctac
     2461 tgcctgctgc catccgcgaa ctgattaaag tctattgccc tcggatcaag tgcacatatc
     2521 ctttcagctc taccgattct tcgccggaga tgcggaagaa gttgactttg caggtgtagt
     2581 aataaacgcc gtccgggcaa tcgctgccgt tgtccttgtg ggtgccgtcc cagttgatgt
     2641 ccttgtcggt cgtctcgaat acgagctggc cccag
//
LOCUS       DAOTON010000365         2671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29848_length_2671_cov_2.968654, whole genome shotgun sequence.
ACCESSION   DAOTON010000365 DAOTON010000000
VERSION     DAOTON010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29848_length_2671_cov_2.968654"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..494)
                     /locus_tag="P5292_07795"
     CDS             complement(3..494)
                     /locus_tag="P5292_07795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRS39069.1"
                     /translation="MKIDSNKVVTVTYRLHANLPAEEQKHIETADQSRPLKFLYGVGM
                     MIPGFERGLEGKGSGDSFSFTIEADDAYGSTDAAAIIDLPIEIFKVEGVIDFDMLKVG
                     NVLPMTDQEGNMLNGKVVSYDEQKVKMDFNHPLAGHILHFSGEIVEVREATPEEMDHG
                     HVH"
     gene            588..1598
                     /locus_tag="P5292_07800"
     CDS             588..1598
                     /locus_tag="P5292_07800"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRS39070.1"
                     /translation="MDSLTHTVIGAVVGDAFAGRRLGKKAMLWGALINNLPDIDVLTS
                     FWMTQASGLLAHRGFTHSILFMVIMTPLLAYGLHGRYKTTSSYQDWLWLSGSNLFIHL
                     FIDALTAYGTGWFEPFSHERVSLNMFFVADPLYTLPFIVGAIALMILRRGSPKRISWH
                     RIGILWGVFYLLFAGWNKYRINQEVTSNVAEQRLPVHDFISTPTPLNNFLWYVIAQGD
                     SGYYIGYRSVFDPKTPMAFTYFPRNDALLTPWKDDPEVGKLIRFSEGIYRLDQEDGQT
                     VFSDMRFGQIGGWMDPKANFVFRFYLDRPDGNDLVIQRGRMEASTPEALKSLWNRMWG
                     QP"
     gene            complement(1599..2384)
                     /locus_tag="P5292_07805"
     CDS             complement(1599..2384)
                     /locus_tag="P5292_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRS39071.1"
                     /translation="MDFKVETMAEFAARGEQPEVLFWVGCAGSFDERAQKVTRAVARI
                     LHHCNVRFAVLGTEESCTGDPAKRAGNEFLFQMQAMMNIQVLNGYGVSRIVTACPHCF
                     NTLRNEYPELGGRYEVLHHSQLLQQLINDGRLRIEGGAFSGKRITFHDPCYLGRANEV
                     YEAPRELIRKLDSTLSEMKRSRANGFCCGAGGAQMFKEPEKGEKDVNIERSEEALALQ
                     PDVIAVGCPFCMTMMTDGVKHFNKESEVKVLDVAELIAQAKDL"
     gene            complement(2416..>2671)
                     /locus_tag="P5292_07810"
     CDS             complement(2416..>2671)
                     /locus_tag="P5292_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRS39072.1"
                     /translation="FRDDKSLLNYISEEELWACTSCNACTEACPVNIDPLSIIVDMRR
                     YLVMEESKVPQELAGMFNKVENNGAPWQFAQADRFNWAND"
BASE COUNT          585 a          730 c          684 g          672 t
ORIGIN      
        1 gatcaatgca cgtgaccgtg gtccatctct tccggggtag cctcgcgtac ttcaacgatt
       61 tctccgctaa aatgcaggat atgtccggca agcgggtggt tgaaatccat cttcactttc
      121 tgttcgtcgt aggagaccac tttaccattc agcatattcc cttcctgatc ggtcatcggg
      181 agtacattgc cgactttgag catgtcgaaa tcgatcactc cttctacttt gaagatctca
      241 atcggaagat caatgatcgc cgcggcatcg gttgaaccat aggcatcatc ggcttcgatg
      301 gtgaagctga aggaatcacc actcccctta ccttccagtc cccgttcgaa tcccgggatc
      361 atcataccga caccgtacag gaacttcaga ggacgggact gatcagccgt ctcgatatgc
      421 ttctgttctt cggccggaag gttggcatgc aggcggtagg ttacggttac caccttgttg
      481 gaatcaattt tcattcgttc tgatttttga taaaaatacg gagaatccgg gttcggctgc
      541 cgcgaaaagg tataaattgc cttcaactgc attttcagcg gatttcaatg gatagtctga
      601 cacatacagt gattggagcg gtcgtggggg atgccttcgc cggccgcaga ttgggaaaga
      661 aagcaatgtt gtggggcgcg ttgatcaaca acctgcctga tatcgacgtg ctgacaagtt
      721 tctggatgac ccaggcttcc ggcttattgg cccaccgcgg gttcacccac tcgatcctgt
      781 ttatggtgat catgacgccc ttgttggcct atggccttca tggtcgttat aagacgacat
      841 cttcctatca ggattggttg tggctaagcg gatcgaatct tttcatccat ttgttcatcg
      901 acgcattgac ggcctatggc acaggatggt tcgaaccgtt ttcgcatgaa cgggtttcgc
      961 tcaacatgtt ttttgtggcc gatccgctct atacccttcc cttcattgtc ggtgccatcg
     1021 cattgatgat cctgcgccgg ggttctccca agcggatcag ttggcaccgg atcggcatcc
     1081 tttggggagt tttctatctg ctcttcgccg gttggaacaa ataccggatc aaccaggagg
     1141 taacaagtaa tgtggcggaa cagcgactac cggtgcatga cttcatcagc acgccaactc
     1201 ccctgaacaa tttcctgtgg tacgtgatcg cccaaggcga tagcggatat tatatcggct
     1261 atcggtcggt attcgatcca aagacgccta tggcgtttac ctatttcccc cgaaacgacg
     1321 cactccttac gccctggaaa gacgacccgg aggtgggtaa actcatccgg ttttcggaag
     1381 gcatttaccg gcttgatcag gaagacggcc agacggtttt cagcgatatg cgtttcggac
     1441 agatcggagg ctggatggac cctaaggcta atttcgtatt caggttctac ctcgaccgcc
     1501 ccgatggcaa tgacctggtg atacaacgcg gacgaatgga agccagtacg cccgaagccc
     1561 tgaaaagtct atggaaccga atgtggggcc agccctgatc aaaggtcctt ggcctgcgcg
     1621 atcagctccg ctacgtccag gaccttcact tcactttcct tgttgaagtg cttgacgccg
     1681 tccgtcatca tggtcatgca aaacggacaa cctaccgcga tcacatcggg ttgaagggcc
     1741 agcgcttctt cactgcgctc aatgttgacg tccttctccc ctttttccgg ctctttgaac
     1801 atctgtgcgc cgcccgctcc gcaacagaaa ccgttcgcac gtgaacgctt catttcggag
     1861 agtgtgctgt cgagttttcg gatgagctcg cgcggcgctt cgtagacttc attcgcgcga
     1921 ccgagataac aaggatcgtg gaacgtgatc cgtttgccgg aaaatgcccc gccttcgatg
     1981 cgcagcctgc cgtcgttgat caactgctgc aacaactgag aatgatgcag cacttcataa
     2041 cgaccaccga gttccgggta ttcgttccgc agggtgttga agcaatgcgg acaagcggtg
     2101 acgatgcgac tcactccgta tccgttcaaa acctggatat tcatcatcgc ctgcatctgg
     2161 aagaggaact cgttgcctgc acgcttcgcc ggatcgccgg tgcaggactc ttccgttccc
     2221 aaaacagcga agcgcacatt gcaatgatga aggatcctgg caacggcacg ggtaactttt
     2281 tgcgcacgct catcaaacga acctgcacat cctacccaga aaagaacctc cggttgctca
     2341 ccccgggcgg caaactcggc catcgtttcc actttgaaat ccattcgtcc gtctcgatta
     2401 gggtgtgtga atcagttaat cgttcgccca gttgaagcga tcggcctgag cgaattgcca
     2461 gggcgcgccg ttgttctcca ctttgttgaa cataccggcc agttcctgcg gcaccttcga
     2521 ttcttccatg accaggtaac ggcgcatgtc gacgatgata ctcaacgggt cgatgttgac
     2581 cggacaggct tccgtacagg cattgcagga ggtgcaggcc cacaattcct cttctgaaat
     2641 atagttgagg agtgatttat cgtcacggaa t
//
LOCUS       DAOTON010000366         2668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29889_length_2668_cov_3.336012, whole genome shotgun sequence.
ACCESSION   DAOTON010000366 DAOTON010000000
VERSION     DAOTON010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29889_length_2668_cov_3.336012"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="P5292_07815"
     CDS             <1..343
                     /locus_tag="P5292_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39073.1"
                     /translation="FLDRGIFPPELAQFVYAYLRDPGASSCSDPGLMRALRKHDERGN
                     DTIVHLEDLPEGSRFKLAGERSRLRFEKGPRLRSRFRCFETDSGREYLVSAIAEVLPL
                     QEAPPAASATS"
     gene            421..1710
                     /locus_tag="P5292_07820"
     CDS             421..1710
                     /locus_tag="P5292_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRS39074.1"
                     /translation="MANTTQATAVHLPVSDMAENLIGSEIIKLAAEINEKIRNGERIF
                     NYTIGDFDPKIFPIPSELNDEIIRAYEQHHTNYPAANGMPELRKTVAEFLRDYEGLNY
                     TADEVLISGGARPLIYATYLTLLDPGDTVIFPVPSWNNNHYTHLSRAKAVFVETRPEN
                     NFMPTAEELRPHLAGATLLALCSPLNPTGTVFSKEQLLAICDLVVEENKRRGPDAKPL
                     YLMYDQIYWVLTFGKTEHYNPVSLRPELRDYTIFVDGLSKSLAATGIRVGWSFGPQRI
                     IDKMKAILSHVGAWAPKAEQVASGKYMSNRSAMDRFVTSFKGEISERLDGFYHGFLEL
                     RKEGFSVNAIPPQAAIYLTVQFELVGKKTPDGRVLNNQKAVTQHILDEAKLALVPFSA
                     FGASDDSNWYRLSVGTCNKHEIGEVFSTLRRVLKALA"
     gene            1749..>2668
                     /locus_tag="P5292_07825"
     CDS             1749..>2668
                     /locus_tag="P5292_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRS39075.1"
                     /translation="MKNNYCIIMAGGIGSRFWPMSRNNFPKQFLDILGTGKTLIQQTF
                     DRFRSICPVENIYIVTNDSYLEHVYQQVPEVPKSQVLAEPQRRNTAPCIAYAAFKIAE
                     RNPDANMIVAPSDHIIHDEASFHAALTKGLAFTAGNDSLLTIGIHPSRPDTGYGYIQF
                     QEEGGDDKFRISKVKTFTEKPNLEMAKFFLKSGEFVWNAGIFLWNLQSILKAFQQHLP
                     DMYHLFEEGKGIYNTPGEADFLRKTYELCTNISIDYGILEKAKNVYVLSAEFGWSDLG
                     TWKSLYEELPKDPAGNAVVGPHVMLDRTSN"
BASE COUNT          603 a          841 c          703 g          521 t
ORIGIN      
        1 ctttcttgat cgaggcattt tccctcccga gctcgcgcag ttcgtttatg cctatctccg
       61 cgatcccggc gcgtccagtt gcagcgatcc cggattgatg cgggccttgc gcaagcacga
      121 tgaacgcgga aacgacacaa tcgttcacct ggaggacctc ccggaaggtt cgcgcttcaa
      181 acttgccgga gaacgcagcc ggctgcgatt tgaaaagggt ccccgcctgc ggtcccgctt
      241 ccggtgcttc gaaaccgaca gcggaagaga gtacctggtg agcgcaatcg cggaagttct
      301 gccgctgcag gaagcacctc cggcggcctc cgcaacgagt tgatccacag cgccatttcc
      361 cgctcccagc gcggcactca ttcgcatatt tggctaactt tgcgctacaa ccaaagtagc
      421 atggcaaaca ccacgcaagc cacggccgtt caccttccgg tttccgatat ggcggaaaac
      481 ctgatcggtt ccgagatcat caagctggca gcggagatca acgaaaagat ccgaaacgga
      541 gaacggatct tcaactatac catcggcgat tttgacccga agatcttccc gatcccttcc
      601 gagctcaacg acgagatcat tcgcgcctac gagcaacacc atacgaacta tcccgcagcc
      661 aacggaatgc cggagctccg gaaaaccgtt gcggaattcc tgcgcgacta cgaaggtctg
      721 aactatacgg ccgacgaggt gctcatctcc ggcggcgcgc gcccgctcat ctacgccacg
      781 tatctgacac tcctggatcc gggcgacacg gtcattttcc cggtaccgtc ctggaacaac
      841 aaccactaca cccacctctc acgcgcgaaa gccgtattcg tcgagacccg gccggagaac
      901 aacttcatgc ctacggccga ggagttgcgg ccccatctgg ccggcgccac cctgcttgca
      961 ctttgttcgc cgctcaaccc gacgggcacg gtcttcagca aagagcaact gctggcgatc
     1021 tgcgacctgg tcgtggaaga aaacaaacgc cgcggccccg acgcgaagcc cttgtacctc
     1081 atgtacgacc agatctactg ggtgctgacc tttggaaaga cggaacacta caacccggtc
     1141 agcctgcgtc cggagttgcg cgattacacc atcttcgtcg acggcctgtc gaagtcgctc
     1201 gccgctaccg gcatccgggt cggctggtcc ttcggtccgc aacgcatcat cgacaagatg
     1261 aaggcgatcc tctcgcacgt cggcgcctgg gccccgaaag cggagcaggt cgcttcgggc
     1321 aagtacatga gcaaccgttc cgcgatggac cgcttcgtta cctccttcaa aggcgagatc
     1381 tccgagcggc tcgacggatt ctaccacggc ttcctggaac tgcgtaagga aggattttcg
     1441 gtgaatgcga ttccacctca ggccgccatt tacctgaccg ttcagttcga actcgtcggt
     1501 aagaaaacac ccgacggccg tgtgctgaac aatcaaaaag ccgtcaccca gcatattctc
     1561 gacgaagcga agcttgcact cgttccgttc tccgcgttcg gagcttcgga cgattcgaac
     1621 tggtaccgct tgtcggtggg cacgtgcaac aagcacgaga tcggtgaagt attttccacc
     1681 ctgcgccgcg tactcaaggc gctggcttaa tctttcttcg gtcgcgtacc ggggaataag
     1741 caattgagat gaagaacaat tactgcatca tcatggccgg cggcattggc agccgcttct
     1801 ggcccatgag ccggaataat ttcccaaaac agtttcttga catcctgggc acaggcaaga
     1861 cactgatcca gcagacgttt gatcgtttcc ggtcgatctg tccggtggag aacatctaca
     1921 tcgtcaccaa cgacagctac ctcgagcatg tgtaccagca ggtaccggaa gtgccgaagt
     1981 cgcaggtcct ggccgagccg cagcgccgca acaccgcgcc ctgcatcgct tacgcggcct
     2041 tcaagatcgc cgagcgcaat ccggacgcga acatgatcgt ggcgccttcg gaccacatca
     2101 tccacgacga agcatccttt cacgcagcgc tgaccaaagg gcttgctttc accgccggca
     2161 acgacagcct cctcacgatc ggcattcatc ccagtcgtcc ggataccggc tatggttaca
     2221 tccagttcca ggaagagggc ggggacgaca agttccggat cagcaaggtc aagaccttca
     2281 ccgaaaagcc gaacctggag atggcgaagt tctttctcaa gagcggcgag tttgtctgga
     2341 acgcggggat cttcctctgg aacctccaat ccattctcaa agccttccag cagcatttgc
     2401 ccgatatgta ccacctcttc gaggaaggca agggcattta caatacaccc ggtgaagcgg
     2461 acttcctgcg caagacctac gaattgtgca ccaacatctc gatcgactac ggtattctgg
     2521 aaaaagcgaa aaacgtttat gtcctgagcg ccgaattcgg ctggagcgat ctgggtacct
     2581 ggaaatcact ctacgaagag ctgccgaaag accccgccgg taacgccgtg gtcggcccgc
     2641 atgtgatgct ggaccgcacc tccaattg
//
LOCUS       DAOTON010000367         2667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29910_length_2667_cov_3.557810, whole genome shotgun sequence.
ACCESSION   DAOTON010000367 DAOTON010000000
VERSION     DAOTON010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29910_length_2667_cov_3.557810"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="P5292_07830"
     CDS             <1..1454
                     /locus_tag="P5292_07830"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HRS39076.1"
                     /translation="PDPITYNSFGMKRLLLLTLLTALLSPLRTLAAGSGYEIKVHING
                     LRDTVCYLGNHFGDKQYVKDTVRTDANGWMTFKGKDPLEGGIYLIVLPNKTYFEVLVD
                     KNDQHFTIETDTLDYISHMKVTGSPENEIFNGHQRFIIQKSMESQAVKARLDANKDNK
                     DSTTYLRKLITDMDKEVKEYRLKLAADHPKSFMAKIIRTMQEPEPPEPPKDANGVITD
                     STFQLRYYKGHYLDNVDFSDDRLLRTPLLQTKIKTYTQQLTVPLPDSIIPAVDTIIIR
                     SRANKEVFKYCVATLANYYETSNIMGYDKVFVHIAEHYYLTNEAYWADSTLKAKIHER
                     VMKIKPNILGTPAKDLVMPDTSFKMHRLYDIKAEYTVLVFWDPTCSHCKKEIPELGAY
                     YDSVKTSGVEVFSVGIESDIELWKQFIRDHKLKWINVSDLYNNTRFRDFYDIYSTPVI
                     YLLDSEKRIIAKRLDVPKLRDFIENDRKAKKGK"
     gene            complement(1556..>2667)
                     /locus_tag="P5292_07835"
     CDS             complement(1556..>2667)
                     /locus_tag="P5292_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRS39077.1"
                     /translation="FGSNRFHRSMNPTEPLKTLSGDLTRNDRKGDVPYNTITTIAESS
                     KRFGLLYLGTDDGLCWISKDDGYTWSKISDGLPQQLWVSRVEPSSHQEGRVYVSLNGY
                     RFDHFLPYVYVSEDFGASWKAIANGLPAEPVNVVREDPSDENILYVGTDNGVYVSLDR
                     GANWMNLTPGLPRTAVHDLVVHPRDPELVIGTHGRSIFILPLTDVRALTDSLRKSPIA
                     FLHAEPVNYQASWGRRPDEFSDPSAPSAAWAYFAQSSGTTRIRLISKAGVLLKEVSDS
                     AEAGVNYVTNDLSLDGATAKKLEAECRKSKKDATFRILPGKDDGKYYLVPGEYKLTFT
                     DANGHSVEGKFEVKDPSAKKESGVPDPESVGPPGK"
BASE COUNT          660 a          768 c          669 g          570 t
ORIGIN      
        1 tccctgatcc aattacgtac aactccttcg gtatgaaacg actcctttta ctgacgctct
       61 tgacggcgct tttatctccg ctgcgcaccc tggcagccgg cagcggttac gagatcaagg
      121 tccacatcaa cggactacgc gatacggtct gttatctggg caatcacttc ggagataagc
      181 agtatgtaaa agacacggtg cgcaccgatg cgaacggctg gatgaccttc aaaggaaaag
      241 atccgctcga gggcggcatt tacctgatcg tccttccgaa caagacttac tttgaggtcc
      301 tggtcgacaa gaacgaccag cactttacga tcgagacgga caccctggat tacatctcgc
      361 acatgaaagt gaccggctcg ccggagaatg agatcttcaa cgggcaccag cgtttcatca
      421 tccagaagtc aatggagtcg caggctgtca aagcccgact cgatgccaac aaggataaca
      481 aggacagcac gacctacttg cggaagctga tcaccgacat ggacaaagag gtgaaagagt
      541 atcgcctcaa actggccgcg gaccatccga aaagcttcat ggcaaagatc atccggacca
      601 tgcaggagcc cgagccgccc gaaccgccga aggacgcgaa cggcgtgatc accgattcga
      661 cctttcagtt gcgttattac aaggggcact acctcgacaa tgtcgatttc agcgacgacc
      721 gcctgttgcg cacaccctta ttgcaaacca agatcaagac ctacacgcaa caattgaccg
      781 ttcccctgcc cgactccatc attccggccg tcgatacgat catcatccgt tcccgcgcca
      841 acaaggaagt cttcaagtac tgcgtcgcca cactcgccaa ctattacgag acctcgaaca
      901 tcatggggta cgacaaagta ttcgtgcaca tcgcggagca ctactacctc accaacgaag
      961 cctattgggc cgacagcacc ctcaaggcca agatccacga gcgggtcatg aagatcaagc
     1021 cgaatattct cggcactccc gcgaaggacc tggtcatgcc cgacacatcc ttcaagatgc
     1081 atcgcctgta tgacatcaag gcggaatata ccgtactggt tttctgggat ccgacctgca
     1141 gtcattgcaa aaaggaaatc ccggagcttg gcgcctacta cgattctgtg aagacgtccg
     1201 gtgtcgaggt gttttccgtc ggtatcgaat ccgacataga gctttggaag cagttcatcc
     1261 gtgatcacaa gttgaagtgg atcaacgtat cggatctcta caacaacacc cgcttccggg
     1321 atttctacga catctactcc acgcccgtca tctacctgct ggattcagaa aagcgcatca
     1381 tcgccaaacg cctggatgtt cccaagctgc gcgacttcat tgaaaacgac cggaaggcga
     1441 aaaaaggaaa gtaacgactc gcgctttcca ataccgcatc aatcaaaagc gggaagcgat
     1501 ctttcgatca cttcccgctt ttgatttttc tcacatcact gagcttcact caagctcatt
     1561 ttccaggagg tccgacggat tcgggatccg gaacaccgga ttcttttttc gctgacggat
     1621 cctttacttc gaacttacct tctaccgagt gtccgtttgc atcggtaaaa gtcagtttat
     1681 actcaccggg aaccaggtag tacttaccgt catcttttcc gggcaggatc cggaaggtag
     1741 catccttctt cgacttccgg cattccgctt ccagtttctt ggctgtcgct ccgtccagac
     1801 taagatcatt cgtgacataa ttaacccccg cttcagcgga atcgctgact tccttcaaca
     1861 aaacacccgc cttactgatg agtcggatcc tggtcgtacc ggaagactgc gcaaagtaag
     1921 cccaggcggc ggaaggcgcg gaaggatccg agaactcgtc aggacgcctg ccccaacttg
     1981 cctggtagtt gaccggctcc gcgtgcagga aagcgatcgg tgatttccgg aggctgtcgg
     2041 tcaacgcacg tacatcggtc aaaggcagaa tgaagatgct gcggccgtgg gtaccgatca
     2101 ccagttccgg atcgcgtgga tgcacgacca ggtcgtgtac ggcggtgcgc gggaggccgg
     2161 gagtcaggtt catccagttg gctccgcggt cgagggaaac atacactcca ttatccgttc
     2221 cgacatagag gatattctca tcgctgggat cctcccgcac cacgttgacc ggctcagcag
     2281 gaaggccatt ggcgatcgct ttccaggagg caccaaaatc ctcgctgacg tacacgtagg
     2341 gcaggaagtg atcgaaacga tacccgttga ggctgacgta aacacggcct tcctggtgcg
     2401 acgaaggttc cacccggctc acccacaact gctgcggaag tccgtccgaa attttcgacc
     2461 aggtataacc atcgtccttg ctgatccagc agaggccatc gtccgtgccg aggtacaaca
     2521 aaccgaatcg tttcgaagat tcagcaatcg tagtaatcgt gttgtaggga acatctccct
     2581 tcctgtcatt tcgggtaagg tcgccggaca aggtcttgag gggctccgta ggattcatcg
     2641 agcgatggaa gcggttggat ccgaagt
//
LOCUS       DAOTON010000368         2663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29962_length_2663_cov_3.902224, whole genome shotgun sequence.
ACCESSION   DAOTON010000368 DAOTON010000000
VERSION     DAOTON010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29962_length_2663_cov_3.902224"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="P5292_07840"
     CDS             <1..424
                     /locus_tag="P5292_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS39078.1"
                     /translation="PVILGRTHSLPPREDDDTDLPYKLPGLLRKAGVVFALSIDGSWQ
                     LRNLPYMAGTTAGYGLTKEEALAAITASPAAILGIADRTGKLETGKDANLLIVSGDLL
                     DMRSSTVEQAFINGRSISLDNVQKQLYQKYSDKYGLTK"
     gene            452..2518
                     /locus_tag="P5292_07845"
     CDS             452..2518
                     /locus_tag="P5292_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013668404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRS39079.1"
                     /translation="MKKSILLATLLAGSGLFVAYGSGNNNPSAKATTTDAPAFDYIAD
                     RFADIQVLRYEVRGWETLSLQQKKLAYYLYEAGLSGRDIFYDQKYRHGLTIRKTLEAI
                     MNTYQGERAGKEWADFETYAKRFFFSNGNHHHYGAEKMMPGCSQAYFTELIRKSDVGQ
                     LPLDGREPAAFTAWIVPIVFDPKVDAKEVDLDAKDVIVASCNNFYSGVTQQEVETYYD
                     GIKEADTSNRSQIGFNTRLVKENGKLVEQVWKSGGMYGAAIDKIIYWLEKAEDVAETP
                     EQKTTIAKLITFYRSGDPKHFDDYSIAWVQDTSARVDFVNGFIEVYQDALQKKGAYES
                     IVSIKDLEATKRIAAISREAQWFEDHSPIMDDHKKKVVTGISAKVITVVGEVGDAGPA
                     TPIGINLPNNEWIREDYGSKSVSLGNIVEAYNYYRSRSPLMDEFGSSPEVIERARKHA
                     AYSGELHTDMHEVIGHASGKINEGVGTTDQTLKNYAGVLEEARADLVALYYIYDPKLV
                     EIGVMESLEVGKAQYDNYIMNGLMTQLNRIEPGKNLEESHMRNRQLISAWAVDKGQQE
                     RVITRENRNGKTYFVINDYAKLRGLFGQLLREIQRIKSEGDFDAGKNLVETYGVRIEP
                     ALHKEVLERYAKLDIAPYMGFIQPKLVPVMAGEEIMDVKVEYPKAFLDQMLEYGKHYS
                     LLPVKN"
BASE COUNT          672 a          707 c          744 g          540 t
ORIGIN      
        1 gccggtgatc ctcggacgta cgcattccct gccgccgcgc gaagacgacg atacggactt
       61 gccctacaaa ctgccggggt tgctccggaa agcgggtgtc gtattcgcgt tgagcatcga
      121 tggcagctgg cagttgcgca acctgcccta catggccggc acgaccgctg gttacggtct
      181 gaccaaagag gaagcgcttg ccgcgattac tgcttcgccg gccgccatcc tcggcatcgc
      241 cgatcgtacc ggtaaactgg agacaggaaa agacgcgaac ctcctcatcg tttcaggcga
      301 tctgctcgat atgcgcagct cgacggttga acaggcgttc atcaacggac gaagcatcag
      361 cctggacaat gtgcagaaac aactctacca gaagtattcc gataaatacg gcttaaccaa
      421 gtaagccgta catcacacca tacacttcat gatgaaaaag agcattctcc tggccacgct
      481 gttggccggc agcggcttgt tcgttgctta cggttccggg aacaacaatc cgtcggccaa
      541 ggccacaact accgatgcgc ccgcattcga ctacatcgcg gatcgctttg ccgacatcca
      601 ggtattgcgt tacgaggtgc gtggatggga aaccctcagc ctgcagcaaa agaaactggc
      661 gtattacctc tatgaagccg gactcagcgg ccgcgatatt ttctatgacc agaagtatcg
      721 ccatgggctg accatccgca agacgctgga agcgatcatg aatacctacc agggcgagcg
      781 cgccggcaag gaatgggcgg actttgaaac ctacgcgaag cgcttcttct tttcgaacgg
      841 caatcaccat cattacggcg ccgagaagat gatgccgggt tgcagccagg cgtactttac
      901 cgagctgatc cggaaatcag acgtcgggca attgccgttg gatggccgcg agcctgcagc
      961 attcaccgcc tggatcgttc cgatcgtttt cgatccgaag gtcgatgcaa aggaagttga
     1021 tctggatgcc aaagacgtga tcgtcgcgtc ctgcaacaat ttttattccg gcgtcaccca
     1081 gcaggaagtg gagacctatt acgacggcat caaggaagcc gacacctcca atcgttcgca
     1141 gatcggattc aatacccgtc tggtgaaaga gaacggcaaa ctggtggaac aggtatggaa
     1201 gtcgggtgga atgtatggcg cggccatcga caagatcatc tactggctgg agaaagcgga
     1261 agacgttgcg gagacgccgg agcagaagac caccatcgct aagctaatca ccttttatcg
     1321 ttccggcgat cccaagcact tcgatgacta cagcatcgcc tgggtacagg acaccagcgc
     1381 gcgcgtcgat ttcgtgaacg gcttcatcga ggtgtatcag gatgccctgc agaagaaggg
     1441 cgcctatgaa agcatcgtat cgatcaaaga cctggaagcg acgaagcgga tcgcggccat
     1501 ttcccgggaa gcgcagtggt tcgaagacca ttcgccgatc atggatgatc acaagaaaaa
     1561 ggtcgtgacc ggcatctcgg ccaaagtgat cacggtagtg ggagaggtcg gcgatgccgg
     1621 cccggccacg ccaatcggga tcaacctgcc gaacaacgaa tggatccggg aagactatgg
     1681 ttccaaatcc gtttcgctcg gaaatatcgt cgaggcctac aattattatc gttcgcggag
     1741 cccgctgatg gacgaattcg gtagttctcc ggaagtgatc gagcgcgcac ggaagcatgc
     1801 cgcctattcc ggcgagttgc atacggatat gcacgaggtg atcggccatg cttcgggtaa
     1861 gatcaacgaa ggcgtgggaa caaccgatca gacgttgaag aactatgccg gcgttctgga
     1921 agaagcacgc gccgacctgg tcgccttgta ttatatctac gatcccaaac tggtcgagat
     1981 cggcgtgatg gaaagcctgg aagtcgggaa agcccagtac gacaattaca tcatgaacgg
     2041 cctcatgacc cagctcaacc gcatcgagcc ggggaagaat cttgaagagt cgcacatgcg
     2101 gaaccggcag ttgatctcgg cctgggcggt cgataaaggt caacaagagc gggtgatcac
     2161 gcgcgaaaac cggaacggca aaacctattt tgtgatcaac gattacgcca aactccgagg
     2221 acttttcggg caattgttac gcgagatcca gcggatcaaa tccgaaggcg atttcgatgc
     2281 cggaaagaac ctggtagaaa cctatggtgt acgcattgaa cctgccttgc ataaagaggt
     2341 tctggaacgc tacgccaaac tggacatcgc gccgtatatg ggttttatcc aacctaaact
     2401 tgttcctgtc atggccggag aagagatcat ggatgtcaag gtggagtacc ccaaggcttt
     2461 cctcgaccag atgctggagt acgggaagca ctattcgctg ctgccggtga agaactgaca
     2521 ggcgtctgac agcaaccata aaaccggaat cggtaaattg gtggaatgta ttggaataca
     2581 ttcttcctgt cccgtctccg gttatttttt ttaatagcct attcatgcgt cttcgctcac
     2641 tcgcaagcaa gtaatccgga ttg
//
LOCUS       DAOTON010000369         2662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_29987_length_2662_cov_2.781358, whole genome shotgun sequence.
ACCESSION   DAOTON010000369 DAOTON010000000
VERSION     DAOTON010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29987_length_2662_cov_2.781358"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="P5292_07850"
     CDS             <1..683
                     /locus_tag="P5292_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRS39080.1"
                     /translation="LEAYSRPRQSGINAIQIEDGDQLLEARLTNGTNEIMLATRKGKL
                     VRFNEETVRPMGRNSIGVKGVTLDGDDDTVIGMVCVSDPLKESVLVVAEQGYGKRSDI
                     DEYRKTNRGAKGVKTINITDKTGNLVAIKVVVENDDLMIITKSGLTIRMGVEDISVQG
                     RATQGVRLIRLEDDDEIASVAKVEVEDKEESQMEDTSAIEGASPEGPSDEAESDGDAS
                     GEAGQENN"
     gene            724..1929
                     /locus_tag="P5292_07855"
     CDS             724..1929
                     /locus_tag="P5292_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39081.1"
                     /translation="MQKLLSLIVVLCLSVSYSFGQKAKLQTAINFYKVPYQQYDKAKE
                     AIDEAVNHEQTKGMAKTWYYKGLIYSALYSSEKYGSLCNNCLVEANDAFKKAMEIEPN
                     NEWAGEIQLNQMQLMSQVFNKGVGQFKEGKYAEALVDFEIAQAISPNDTATILNSAYA
                     AERAGNKAKAKQYYEKLVSMNYQDDKSYAALSNLYREDGNTDKALEVLRQGRTAMPES
                     INLMLAEINILLSTGKSQEATTALDAAIQKDPKNPSLYMALGSTFDNLANPKGADGKD
                     KAKPAQYNDYMSRAEAAYKKGLEIQPDNYEINYNLGAMYFNQGADMANAANQLTNNAE
                     FEKAKVKYEDKFKAAAPYLEKSLEANPRKTEDDQSLYQGTLLSLKQLYVRLNETEKYN
                     RIKNLLEQK"
     gene            2260..>2662
                     /locus_tag="P5292_07860"
     CDS             2260..>2662
                     /locus_tag="P5292_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39082.1"
                     /translation="MKSICCHTKLLQPAGPDTPDLCLNPDCGNYLRPTAIYSPRVWNR
                     VLAVGFFLFLFMLTMDDRSTANRATELTSSMQKMRGKTVRPLSPESLREELDHQHVIC
                     PEQVQAQILLESGNLESYLARRTNNLLGMRFP"
BASE COUNT          729 a          635 c          725 g          573 t
ORIGIN      
        1 cgctggaggc gtattcccgt ccgcgtcagt cgggcatcaa cgcgatccag atcgaagacg
       61 gcgaccagtt gctggaagcc cgccttacga atggcaccaa cgagatcatg ctcgcgacac
      121 gtaagggtaa gctcgtccgg ttcaacgaag agactgtgcg tccgatgggg cgaaattcca
      181 ttggtgtaaa gggtgttacg ctcgacggag acgatgatac cgtaatcggc atggtatgtg
      241 tcagtgaccc gctcaaagag tcggtattgg ttgtagcgga acaaggttat ggcaagcgtt
      301 ctgatatcga cgaataccgg aaaacgaacc ggggggccaa gggcgtaaag accatcaaca
      361 taaccgacaa gaccggcaac ctggttgcga tcaaggtggt agtggaaaac gacgacctga
      421 tgatcatcac caaaagcgga ctgaccattc ggatgggggt agaggacatc tccgttcagg
      481 gcagggcaac ccagggcgtt cgactgatcc gcctggaaga tgatgatgag atcgcctcgg
      541 ttgcgaaggt ggaagtggag gataaggaag aatcgcagat ggaagatact tccgccattg
      601 agggcgcttc gccggaagga ccttccgatg aagcggagtc agatggggat gcgagcggag
      661 aggccgggca ggaaaataat tgaactgata ttcgtttaac ataataatac gcaatcggaa
      721 atcatgcaaa aactgctttc gttgatcgta gtgctttgtt tgtctgtaag ttattccttc
      781 ggacaaaaag cgaaacttca aacggctatt aatttctaca aggtccctta tcagcaatac
      841 gataaggcaa aagaggccat cgatgaggcc gtcaaccatg agcagaccaa ggggatggcc
      901 aagacctggt actataaagg gctgatctat tcggctctgt attcaagtga gaagtatggt
      961 tcactttgca acaattgcct ggtggaagcc aatgacgcgt ttaaaaaggc catggaaatc
     1021 gagcccaata atgaatgggc tggtgaaatt caattgaatc aaatgcagtt gatgagccag
     1081 gtcttcaaca aaggtgttgg tcagttcaaa gaaggcaagt acgcggaagc gctggtggat
     1141 ttcgagattg cacaggccat tagtccgaac gataccgcta cgatcctgaa ttcagcttat
     1201 gccgctgagc gggcgggaaa caaggcgaag gcgaagcaat actatgaaaa gctggtttcc
     1261 atgaattatc aggacgacaa gtcttatgcc gccttgtcga acctgtaccg tgaggatggc
     1321 aataccgata aggctttgga ggttctgcgc cagggacgta ccgccatgcc ggaaagcatc
     1381 aatctgatgc tcgccgagat caacatcctg ttatcgaccg gtaagagtca ggaagccacc
     1441 acggcactgg acgcggccat tcaaaaggac cccaagaacc cgagccttta catggccttg
     1501 ggcagcacgt ttgacaactt ggccaatccc aaaggtgcgg atggcaagga caaggcgaaa
     1561 ccggctcagt ataacgatta catgagccgt gctgaagcag cctataagaa gggtctggag
     1621 atccagccgg acaattacga gatcaactat aacctgggag ctatgtactt caaccagggc
     1681 gccgatatgg ccaatgcagc gaatcagttg accaataatg ccgaattcga gaaggcgaag
     1741 gtcaaatatg aagacaaatt caaggctgca gcgccgtatc tggaaaagtc gctcgaagcg
     1801 aatccccgga agacagagga tgaccagtcc ttgtaccaag gaaccttgtt gtcgttaaaa
     1861 cagctgtacg ttcgattgaa tgagacagag aagtataacc ggatcaagaa tctgttggag
     1921 cagaagtaga ctgctgttcg agcgactcga taggatattt gaacacttca cgttcaggac
     1981 cccggccagg agacagactt ccaggccggg gttcgctgtt tctatggaat agggtgtttc
     2041 gcttaattat aacttaacat aatgttaatt ataagactga atgggttgta tttttttgaa
     2101 aatgaggatg atccgctggc ggcaagatcc cggctgttcg cccaatcagg ttatccgttt
     2161 gaccaaataa ggttccggtt cggctggctg ccaacctact tttgatctga cttccccgac
     2221 acaccatgaa tacccgagtc cccctaaaga ttcaacgtca tgaagtcgat ttgctgtcat
     2281 accaagttgc tgcaacctgc cggtccggac acccccgatc tgtgcctcaa cccggattgc
     2341 ggaaactacc tccgcccgac cgctatctat agcccccggg tctggaaccg cgtcctggcg
     2401 gttgggttct tcttgttcct ctttatgctg accatggatg atcgttctac agccaaccgg
     2461 gctacagaac tcaccagcag catgcagaaa atgcgcggaa aaacggttcg tccgttaagc
     2521 ccggaaagcc tgcgtgaaga attggatcat cagcatgtga tttgtcctga acaagtccag
     2581 gcccagatcc tgctcgagtc cggtaacctg gaatcgtatc tggcccgccg aaccaacaac
     2641 ctgctgggca tgcgcttccc gt
//
LOCUS       DAOTON010000370         2657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30054_length_2657_cov_2.380477, whole genome shotgun sequence.
ACCESSION   DAOTON010000370 DAOTON010000000
VERSION     DAOTON010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30054_length_2657_cov_2.380477"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1785)
                     /locus_tag="P5292_07865"
     CDS             complement(<1..1785)
                     /locus_tag="P5292_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRS39083.1"
                     /translation="MHPIKAALQQRILVIDGAMGTMIQRYRLGEDGYRGDRFRDFPHS
                     LKGNNDLLCLTRPEIVRAIHREYLEAGADIIETNTFNAQALSLADYHMEDLAYEINLA
                     AARLAKEAVAGFAADHPNSTPRFVAGALGPTNKTASLSPDVNDPGFRAVSFHDLVEAY
                     AVQTRGLIDGGADVLLVETIFDTLNAKAALFAIETVFDEKGVQLPVMVSGTITDASGR
                     TLSGQTVEAFLISVSHLDLLSVGLNCALGAKEMRPHIEELAQKAPFFVSAYPNAGLPN
                     QFGEYDETPDHMGHQIEDFLRSGFLNIVGGCCGTTPDHIRHIAALAKKTPPRVIPSVE
                     PWLRLSGLEAVVLRPDSNFMNVGERTNVTGSRKFLRLIKEGNYEEALSVARDQVEGGA
                     QAIDVNMDEGMLDAADAMTRFLHLMASEPDIAKLPVMIDSSKWHVIEAGLQCTQGKSI
                     VNSISLKEGEEEFIRQAKLVRRYGAAVIVMAFDEKGQADTLPRRIEICERAYRILTEK
                     VGFPPQDIIFDPNIFPVATGMDEHRRNALDYFEATRWIKRHLPHAKISGGVSNVSFSF
                     RGNDRVREAIHAAFLYHGIQAGMDMGIVN"
     regulatory      complement(1892..2015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2156..>2657)
                     /locus_tag="P5292_07870"
     CDS             complement(2156..>2657)
                     /locus_tag="P5292_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39084.1"
                     /translation="VSRFRDEIIKDDYEMFFVINIRRPMTDTAAKIMDMIRDIEESAR
                     IRVTGLVNNTNDATSSCLTFGSGETEDYLIGLDFNVGTIAPELNGSVLYPNPTNGLLN
                     VFFPNMKGQCTVRLFDGMGRQLQMLNNDNLVSLQLDLGAYNNGIYFVKVETPNGSITK
                     PIVLQR"
BASE COUNT          533 a          703 c          812 g          609 t
ORIGIN      
        1 gttgacgatg cccatgtcca tgcctgcctg tatgccgtga tagaggaagg ccgcatggat
       61 ggcttcgcgt acacgatcgt tgccgcgaaa ggagaacgaa acgttactga cacctccgct
      121 gatcttggca tgtggaagat gtcgcttgat ccaacgggta gcctcgaagt aatcgagcgc
      181 gttgcgccgg tgctcatcca tgccggtggc gaccgggaag atgttcgggt cgaagatgat
      241 atcctgcggc gggaaaccga ctttctccgt aaggatccgg tacgcgcgtt cgcagatctc
      301 gatacgacgg ggtagtgtat ccgcctggcc cttctcgtcg aaggccatga cgatcaccgc
      361 ggcgccatag cggcgtacga gcttcgcctg ccggatgaac tcttcttcgc cttctttcag
      421 ggagatcgag ttcacgatcg acttcccctg ggtgcattga aggccggctt cgatgacgtg
      481 ccacttggat gagtcgatca tcaccggcag tttggcgata tcgggttccg aggccatgag
      541 gtgcaggaag cgggtcatgg cgtcggccgc gtcgagcatg ccttcgtcca tgttcacgtc
      601 gatcgcctgt gctccgcctt cgacctggtc gcgggcgacg ctgagcgctt cttcgtagtt
      661 tccttccttg atcagtcgca ggaacttgcg tgagccggtg acgttggtgc gctcgcccac
      721 gttcatgaaa ttcgaatccg gtcgcagcac gacggcttcg agcccgctga ggcgcagcca
      781 gggttctacc gaaggaatga cgcgtggcgg tgttttcttc gccagcgcgg cgatgtggcg
      841 gatatgatcg ggcgtggtcc cgcagcagcc accgacgatg ttgaggaaac cactgcgcag
      901 gaagtcttcg atctggtggc ccatgtggtc gggcgtctcg tcgtactcgc cgaactggtt
      961 gggcagtccg gcgttgggat acgcgctcac gaagaagggc gccttctggg cgagttcttc
     1021 gatgtgcggg cgcatctcct tagcgccgag cgcgcaattc aatcctacac tcagcagatc
     1081 gaggtgcgat acggaaatga gaaacgcttc caccgtttgt ccgctgaggg ttcgtccgct
     1141 ggcatcggtg atggtgccgg aaaccatgac gggcaactgc acgccttttt cgtcgaagac
     1201 ggtttcgatg gcgaagagag ccgccttcgc gttgagcgta tcgaaaatgg tttcgacgag
     1261 caaaacgtcg gcaccgccgt cgatgaggcc gcgggtttgc accgcgtacg cttcgaccag
     1321 gtcgtgaaag ctcacggccc ggaagccggg atcattcaca tcgggtgaga gcgatgctgt
     1381 cttgttggta ggtccgagcg cgcctgcaac gaagcggggc gtactgtttg gatggtccgc
     1441 cgcgaaaccg gccacggctt ctttcgcgag gcgagcggcg gcgaggttga tctcgtacgc
     1501 aaggtcctcc atgtggtaat cggccaggga gagggcttgc gcgttaaagg tgttggtctc
     1561 gatgatgtcg gctccagcct cgaggtattc ccggtggatg gcgcgcacga tctccggtcg
     1621 ggtcagacag aggaggtcat tattgccctt cagcgaatgt ggaaaatccc ggaagcgatc
     1681 gccgcggtaa ccgtcttcgc cgagacgata ccgttgaatc atggttccca tggccccgtc
     1741 gatgacgagg atacgttgtt gcagtgcagc tttgatcgga tgcataatcg ttcaggttcc
     1801 ttccgctccg cgaccccagg agaaaaaaaa atccctcctg cgctagtcag aagggatcgt
     1861 atggcgattt cgattgggca acgatcattc tgacttatct ctctccacca aatggcggag
     1921 tgcgaggtgg caccttattc tattgttttt caacaattta gaaccggttg ccaaggcttc
     1981 acagggcgca atccctcagc ctttctggat aagccgtctc gtcgaaagac gagaggaggt
     2041 ggaaagaact ggtgcaaaga taacgaattt gctttcgtct ataagtgact cgcgcaaata
     2101 cttaaaaaca cgaaagcctc cccgaaggaa ggctttcatg tgatgatcat tgggattaac
     2161 gctgcaacac gatcggcttg gtgatggagc cgttcggagt ttccactttt acgaagtaga
     2221 taccgttgtt gtaagcaccc aggtcgagct ggaggctgac gaggttatcg ttgttgagca
     2281 tttgcaactg gcggcccatg ccgtcgaaca gacgaaccgt gcactgaccc ttcatgttcg
     2341 ggaagaacac gttgagcagt ccattggtgg ggttcgggta gagcaccgag ccgttgagtt
     2401 ccggcgcgat ggtaccgaca ttgaagtcga ggccgatcag gtagtcttcc gtttcaccgg
     2461 aaccgaaggt caggcaggag ctggtggcgt cgttggtatt gttcaccagg ccggtcaccc
     2521 tgatgcgtgc gctctcttca atatcccgga tcatatccat gatttttgca gccgtatccg
     2581 tcatgggacg tcggatgtta atgacaaaga acatttcata gtcatccttt atgatttcgt
     2641 ccctgaacct tgaaaca
//
LOCUS       DAOTON010000371         2655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30086_length_2655_cov_2.581923, whole genome shotgun sequence.
ACCESSION   DAOTON010000371 DAOTON010000000
VERSION     DAOTON010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30086_length_2655_cov_2.581923"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="P5292_07875"
     CDS             complement(<1..566)
                     /locus_tag="P5292_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HRS39085.1"
                     /translation="MKFFIDTANLDQIREAQDLGVLDGVTTNPSLMAKEGITGEANIL
                     KHYVDICNIVTGDVSAEVIATDYAGIVSEGERLAKLHPQIVVKVPMIKDGIKAIRYFS
                     NKGIKTNCTLVFSAGQALLAAKAGATYVSPFLGRLDDINYDGVELISQIVHIYRMYDY
                     PTQVLAASIRNPLHIVRCAEAGADVATCP"
     gene            complement(697..794)
                     /gene="ffs"
                     /locus_tag="P5292_07880"
     ncRNA           complement(697..794)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5292_07880"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            916..1449
                     /locus_tag="P5292_07885"
     CDS             916..1449
                     /locus_tag="P5292_07885"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRS39086.1"
                     /translation="METPRSQTKLAMNYGAFYGLSTIIVMLVFYFAGSNMQSKWPQWI
                     GYLLLITFMVLGIKSFRDEEGGGFISFGRSLGTGILISIFGSIITGAFTVVLFTFIAP
                     DMTQRILEMTQQNLTEQGMSKEQINMSIEWARKFMTPMWLFVFSVVGGAFMGLLFGLF
                     ISIFLRREQNNPFQSNA"
     gene            1453..2409
                     /locus_tag="P5292_07890"
     CDS             1453..2409
                     /locus_tag="P5292_07890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS39087.1"
                     /translation="MDISVVIPLFNEEESLPELHDWIVRVMDANKYSYEILFIDDGSK
                     DRSWEVITGLSNRNAHVRGIRFRRNCGKSAALSEGFAATSGDVIITMDADLQDSPDEI
                     PDLYRMIREEGFDLVSGWKKKRYDPISKTIPSKFFNWTTRKMSGIDNLHDFNCGLKSY
                     RKEVVKSIEVYGEMHRYIPVIARWAGFRKIGEKVVEHRSRKYGVTKFGWERFINGFLD
                     LLSITFVSKFGKRPMHLFGFLGTIMFLIGFFSAAYLGISKLLAVYHNVPARLITDRPS
                     FYIALTAMIMGTFLFMAGFLGELISRNSPDRNRYLVDQRIQP"
     gene            2443..>2655
                     /locus_tag="P5292_07895"
     CDS             2443..>2655
                     /locus_tag="P5292_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39088.1"
                     /translation="MNIVLIGPAFPLRGGIADFNEALANALIAAGHTVRIHSFYLQYP
                     RLLFPGKSQYATAGTTGPALDIRPTIG"
BASE COUNT          572 a          693 c          743 g          647 t
ORIGIN      
        1 ggacaagtcg cgacgtcggc accggcttcg gcacagcgga cgatgtggag cggattacgg
       61 atactggccg cgagtacctg tgtcggatag tcgtacatgc gatagatgtg cacgatctgg
      121 cttatcagtt cgacgccgtc gtagttgatg tcgtctaacc gaccgaggaa cggtgatacg
      181 taggtagcgc ctgctttggc ggcgagtaat gcctgccctg cggaaaagac cagggtgcaa
      241 ttcgtcttga tgcctttgtt ggaaaagtag cggatggcct tgatgccgtc cttgatcatc
      301 gggaccttga caacgatctg cggatgcagt ttggccaggc gctccccctc ggaaacgatg
      361 cccgcataat cggtggcgat cacttcggcg cttacgtcac cggttacgat gttgcagata
      421 tcgacatagt gtttcaggat gttcgcttcg ccggtgatcc cttccttggc catgagggaa
      481 gggttggttg ttacgccgtc gaggacgccg aggtcctgtg cttcacggat ctgatcgaga
      541 ttggctgtgt cgatgaagaa tttcataagc ggatggatga tggaaggagg gaacgttagg
      601 cgttgggttt tcgcccgaac cggaaaaaca gtcggacgaa cgatcgcccg aagatacccg
      661 ctgctccggc tcggcggagg ggtgttgaaa taaaaatacg ggtaagcttt ctatatcgca
      721 ccgctcaacc gcgtatcctt gctccgttcc cggcctgggg aggttggcag gagctggtcg
      781 tataggactt acccgtgccg caaaggtaca aaatcgttcg tgaattcaac atcggtagtc
      841 gaaaacaggc gctcgggcgc tttggccgat tggcggaatt agagtaggtt tgtaggccta
      901 ttttctttaa aatcaatgga aactccccgt tcacagacca agctggcaat gaattacgga
      961 gccttttatg gcttgtcgac catcattgtc atgctcgttt tttatttcgc cggttcaaat
     1021 atgcaatcga agtggccgca atggatcggc tatctcttgc tgatcacgtt catggttttg
     1081 ggcatcaaga gttttcggga tgaggaaggc ggaggattca tctccttcgg ccgctcgctg
     1141 ggtacgggta tcctgatctc gattttcgga tccatcatca ccggtgcatt cacggtggta
     1201 ctcttcacct tcatagctcc ggacatgacc cagcggatcc tggagatgac acaacagaat
     1261 ctcaccgagc aggggatgag caaagaacag atcaacatgt ccatcgagtg ggcacggaaa
     1321 ttcatgaccc ccatgtggtt gttcgttttc tccgtcgttg gtggcgcttt catggggctg
     1381 ctgttcggac tgttcatctc gatcttccta cgccgcgagc agaacaatcc ctttcaatcc
     1441 aacgcctgat ccgtggacat cagtgtagtc attccgctct tcaacgaaga agagtcgctc
     1501 cccgagttgc acgattggat cgtacgggtc atggacgcga acaagtacag ctatgagatc
     1561 ctgttcatcg acgacggcag caaggatcgc tcctgggaag tgatcaccgg cctttcgaat
     1621 cgaaacgccc atgtgcgggg gattcgtttc cgcaggaatt gtggcaagtc cgccgccctc
     1681 agtgaaggct tcgccgccac cagcggtgat gtgatcatta ccatggacgc ggacctgcaa
     1741 gacagtcccg acgaaatacc ggatctgtat cgcatgatcc gcgaagaggg attcgatttg
     1801 gtttcgggct ggaagaagaa gcggtatgac ccgatctcca agacgattcc gagcaagttc
     1861 ttcaactgga cgacccggaa aatgtcgggt atcgacaacc tgcacgattt caactgtgga
     1921 ttgaagtcct accggaaaga ggtggtcaag agtattgaag tgtatggcga aatgcaccgg
     1981 tacattcccg tcatcgcacg atgggccggc ttccgcaaga tcggggagaa ggtggtcgag
     2041 caccgttccc gcaagtacgg cgtgaccaaa ttcggttggg agcgtttcat caacgggttc
     2101 cttgacctgc tgtcgatcac cttcgtcagc aaattcggaa agcgacccat gcacctgttc
     2161 gggttcctgg gtacgatcat gttcctgatc ggctttttct ctgcggctta tctgggtatc
     2221 agcaaattgc tggcggtcta tcacaatgtc ccggcccgac tgattactga tcgtccatcg
     2281 ttctatatcg cgctcacggc gatgatcatg ggaaccttcc tgttcatggc gggttttctg
     2341 ggcgagctca tttcccgcaa ttcaccggat cgcaatcgct acctcgtcga ccagcgcatt
     2401 caaccctgac cgatcccttg ttccgacgaa gccagactcg ccatgaacat cgtactgatc
     2461 ggtcccgcat ttccccttcg aggtggtatc gccgatttta acgaagccct ggcgaatgcc
     2521 ttgatcgcag cgggtcatac agtgaggatc cattcgttct atttgcaata tcccaggctg
     2581 ttgtttccag gaaagtcgca gtacgcgact gccggaacca ctggccccgc acttgatatt
     2641 cgccccacta tcgga
//
LOCUS       DAOTON010000372         2653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30117_length_2653_cov_3.825635, whole genome shotgun sequence.
ACCESSION   DAOTON010000372 DAOTON010000000
VERSION     DAOTON010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30117_length_2653_cov_3.825635"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="P5292_07900"
     CDS             complement(<1..219)
                     /locus_tag="P5292_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRS39089.1"
                     /translation="MRIHKEGYATISLTIVVLLALNLLAGFFLSEIHLLRQLLFVVSI
                     VLFLLVLQFFRHPQRKITRNEKQIIAPAD"
     gene            complement(285..1574)
                     /locus_tag="P5292_07905"
     CDS             complement(285..1574)
                     /locus_tag="P5292_07905"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRS39090.1"
                     /translation="MAVAEKAAQKQAQPKETTNPYESMIQRFDVAAKIIGLDEETYNI
                     LKTPQRQYVVSLPVAMDNGKVQVFEGYRIIHNTSRGPSKGGIRYALEVNQDEVKALAA
                     WMTWKCAIADIPYGGAKGGITVDASKLSLGELERLTRAYTRAMVDVFGVDKDIPAPDV
                     ATGPREMAWIVDEFSRMQGSFQPGVVTGKPLHLGGSEGRVEATGRGVNTAALEAMRQM
                     RLDPRKATAAVQGFGNVGSISAKYMEDAGLRIVAISDHTGAFSNSKGISIGDAIKYRN
                     ANNGVLKGFTGGKLISNEELLALDVDVLAPCALENQIRQANAADVKAQLIVEGANGPT
                     TAGADKILQEKGIIVVPDILANGGGVTVSYMEWTQNRFGYYYAEDEINKRADRSMSRA
                     FENVWAVGKKYKVSMRIAAYIFALEKVAKAVQSRGHY"
     gene            complement(1717..>2653)
                     /locus_tag="P5292_07910"
     CDS             complement(1717..>2653)
                     /locus_tag="P5292_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39091.1"
                     /translation="GLGLLRRTFTVSGVPDIPSGLILLSLAFGTHLFYYALNEPSMSH
                     VYSFAAIAAWIYIGQQYARSPDRSRMFQLAFLLGLLIVIRPVNGMVLFSLPFIAGRNG
                     WLNIMSSLKSRPWRFVSFGSIVLGWMLFQVLLNYAQSGSLILYTYGEQRLDLLHPHLF
                     DFLLSYRKGLFIYTPWCLLAVLVSLVLFRDDPWRAISGIGFLVLVIWVLSSWSLWWYG
                     GSFSSRPMVDYLPFFALLLGVAWSALRKNRIVRIGLPAVLFFVVFVNQVQTYQYRYNI
                     IHWDSMDREHYFRVFLRVDQLGQKTNPNADLLENR"
BASE COUNT          619 a          696 c          751 g          587 t
ORIGIN      
        1 gtctgcaggg gcgatgattt gtttctcgtt ccgggtgatc ttgcgttgcg ggtgacggaa
       61 gaactggagg accaggagga acagaacgat cgacacgacg aacaacaact ggcgcaacag
      121 gtggatttcc gagaggaaaa atccggccag caggttgagg gcgaggagga cgacgatcgt
      181 gagcgagatc gtagcgtagc cttctttgtg gatccgcata ggcagaggcg agttatgcaa
      241 aagaagaaag gcgtcggggg tgacgccttt ccggtgaggt tagatcaata atggccgcgc
      301 gactgaaccg ccttggcgac tttctccagc gcgaagatat aggcggcgat gcgcatcgaa
      361 actttgtact ttttacctac agcccagacg ttctcgaatg cgcgtgacat ggaacggtcg
      421 gcgcgcttgt tgatttcgtc ttccgcatag tagtagccga accggttctg cgtccattcc
      481 atgtaggaaa cggtcacgcc tccaccattg gccagaatat cgggcacgac gatgatgccc
      541 ttttcctgca ggatcttatc ggccccggcc gtagtgggtc cgttggcgcc ttccacgatc
      601 aactgggctt tcacatccgc tgcattggct tgtcggatct gattttccag tgcacagggt
      661 gcgagcacat ccacatccag cgcgagcagt tcttcgttgg agatcaactt cccacccgtg
      721 aatcctttca gtactccgtt gttggcgttg cggtatttga tcgcgtcgcc gatggaaatg
      781 cccttcgaat tggagaaggc tccggtatga tcgctgatgg cgacaatgcg caggccggcg
      841 tcttccatgt acttggcgga aatgctcccg acattgccga atccctgcac ggcggcagtg
      901 gcttttcgcg ggtcgagtcg catctggcgc atggcttcga gcgccgcggt gtttactccg
      961 cgtccggtcg cttcaacacg accttcggaa cctccgaggt gaagcggctt gccggtcacg
     1021 acacccggtt ggaaagaacc ttgcatacgg gagaattcat caacgatcca ggccatctcg
     1081 cgcggtcctg tcgcgacatc gggagcaggg atgtctttgt cgacgccgaa aacgtccacc
     1141 atcgcgcggg tgtaggcacg tgtcaatcgc tcgagttctc cgagcgaaag tttggaagcg
     1201 tcaaccgtga taccgccttt cgcaccgcca tacgggatat cggcgatggc gcatttccac
     1261 gtcatccagg cagcaagtgc ttttacttcg tcctgattga cttccagtgc atagcgaatg
     1321 ccgcctttcg acggtccgcg actggtgttg tggatgatgc ggtaaccttc gaacacctgc
     1381 acttttccgt tgtccatcgc aaccggaagg ctgacgacat actgacgttg cggggttttc
     1441 aggatattgt acgtctcttc atcgagaccg atgatcttcg cggctacatc gaaccgttgg
     1501 atcatggatt cataaggatt ggtagtctcc ttgggttgcg cttgtttctg cgctgctttt
     1561 tctgccacag ccatgctgct ttcctattgg ggcgacaaaa gtaggtattc cgggggattg
     1621 ggggaattta ttcgggtaaa ccgccccgtt cattccgcga aattggccta aaacagggtg
     1681 ttttcggcga ttatcttgat aacataggtg ctcgactcaa cggttttcca ggaggtcggc
     1741 attgggattt gttttttgtc ccaattgatc cacccgcagg aacacccgga aatagtgctc
     1801 ccggtccatg ctatcccaat ggatgatgtt gtatcggtac tgataagtct gcacctgatt
     1861 gacgaaaaca acgaagaaca gtacagcggg taacccgatc cggactatcc tgtttttacg
     1921 aagggcagac caagccacgc ccaacaagag tgcaaagaac ggcaggtagt cgaccatggg
     1981 acgcgagcta aagcttccgc cgtaccacca gagcgaccag gatgatagca cccagatcac
     2041 caacaccagg aatccaatgc cgctgatggc gcgccatgga tcatcgcgga acaagacgag
     2101 cgaaacaagt acggccagta aacaccaggg tgtataaatg aagagtcctt tccggtagct
     2161 caacaaaaaa tcgaacaggt gtgggtgcaa caggtccaga cgttgttcac catacgtgta
     2221 aaggatcaga cttcctgatt gggcgtagtt cagcagcacc tgaaacagca tccaacccaa
     2281 aacgatcgaa ccgaaggaga cgaaccgcca tggtcggctt ttcagcgaac tcatgatgtt
     2341 gagccagccg tttcggcccg cgatgaaggg aagggaaaag agcaccatcc catttaccgg
     2401 acgaatgacg atcaacagtc cgagtaagaa ggcaagttga aacatgcggg acctgtcggg
     2461 actcctggca tactgttggc cgatgtagat ccaggctgct atggctgcaa aggaatagac
     2521 atgcgacatc gaaggttcgt tcagggcgta gtaaaacagg tgtgtcccga acgcgaggct
     2581 caagaggatc aatccggaag gaatatccgg cactccggaa acagtgaaag tccgtctcaa
     2641 cagcccgagc ccc
//
LOCUS       DAOTON010000373         2650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30162_length_2650_cov_3.723314, whole genome shotgun sequence.
ACCESSION   DAOTON010000373 DAOTON010000000
VERSION     DAOTON010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30162_length_2650_cov_3.723314"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="P5292_07915"
     CDS             complement(<1..748)
                     /locus_tag="P5292_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HRS39092.1"
                     /translation="MKRLLLLAFALLTLLNARAAFLLIPMDESQTNHLKAYGIAYYVL
                     QRDLDVQWLLNYRGGSFLIRQVPAIEQECSIRGVSFQVIADAQAASILAAISDPEVNQ
                     DVVKLEKAPKIAVYSPKNKMPWDDAVTLALTYAEIPYEVVYDEEVLQGKLPLYDWLHL
                     HHEDFTGQYGRFWSAYRNAPWYQEDVRIQESMAAKLGYQKVSQMKLAVALRIRDFVVG
                     GGYLFAMCSATDSYDIALSAEGVDICESMFD"
     gene            complement(841..>2650)
                     /locus_tag="P5292_07920"
     CDS             complement(841..>2650)
                     /locus_tag="P5292_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRS39093.1"
                     /translation="FPLLLAQGVEGIAVGLACRILPHNFVELLEASIDLLRGKKVNIL
                     PDFPTGGIADFSKYNEGLRGGRIRIRARIEKKDRKTLIIREIPFGTTTTSLIDSILAA
                     NDKGKIRIKKVEDNTASNVEIVLTLHSDSMEDLDKTIDALYAFTDCEISISPNACVIV
                     DDKPKFLTVNEILRHNTDQTLNLLKRELEIRKSELEEEWHFSSLVKIFIEKRIYRNIE
                     ECTTWESVIETIDEGLKPYKKKFKRAITRDDIISLTEIKIKRISKYDVKEQDEKIRGL
                     EAQLEEVEHHLAHIRQYTINWFKDLLKKYGKGRERKTKIDTFEQVVASEVIINNQKLY
                     VNRDDGFAGFGMKKDEFVSECSDLDEILVIRGDGTMIVTKVDDKKYVGKDVRYISVFR
                     KSEPEPVFNLIYQDGRGGNIMVKRFTVGGTTRDKEYVLTKGNDGSKILYLSAMPNESG
                     ETVRVILRPKPKLRITQLDVDFNQFEVKGRGTIGNILTKNSVQRVTKIAEAPATPPAK
                     QLTIPAGESKAGKAEATKPATKSAAAKKPAPVVKGKSPSKAPKKAPPLNAESAVTVEW
                     DLSAGSTNTRSDRARILEEIEEKTEKRKKNQMKMDF"
BASE COUNT          527 a          750 c          692 g          681 t
ORIGIN      
        1 cgtcgaacat gctttcacag atatcaacac cttccgccga aagcgcgatg tcatacgaat
       61 cggtagccga acacatcgcg aacagataac caccgccgac cacgaaatcc cggatcctga
      121 gggcgacggc cagtttcatt tgcgacacct tctgataacc gagtttggcg gccatggatt
      181 cctgaatccg cacgtcttcc tggtaccagg gtgcattccg atacgcggac caaaaccggc
      241 cgtattgacc ggtaaaatct tcgtgatgca ggtgcagcca atcgtacaag ggcaacttcc
      301 cctgtagcac ctcttcatca tacaccacct cgtaggggat ctccgcgtac gtcagggcaa
      361 gggtgaccgc atcgtcccag ggcatcttgt tcttgggcga atacaccgcg atcttcggcg
      421 ccttctccaa tttcaccaca tcctggttga cttcaggatc ggatatcgcc gccaggatgg
      481 acgctgcttg cgcgtcggca atcacctgga aactcacccc acggatgctg cactcctgct
      541 cgatagccgg gacttgccgt atcagaaagc tgccgccccg gtaattcagc agccactgga
      601 cgtccaaatc acgctgaaga acataatagg caatgccgta cgctttcagg tgattcgtct
      661 gcgattcgtc catcgggatc aacagaaacg cagctcgcgc gttcagcagc gtgagcaggg
      721 caaatgcgag tagcaacagg cgcttcatgt gacgggactg ggactgaaat tacaacgatc
      781 gatatgaagg gatattgaag gagcggtccg gatcgggact gcacccgccg gaccgttcct
      841 ttagaaatcc atcttcatct gattcttctt tcgcttctcg gttttctctt caatttcctc
      901 gagaattcgg gcacgatctg accgggtgtt tgtacttccg gcggatagat cccactcaac
      961 ggtgaccgcg ctttctgcgt tgagcggtgg ggcttttttt ggcgccttcg aggggctctt
     1021 ccctttgaca accggagcag gctttttcgc tgccgccgat ttcgtagcag gttttgtcgc
     1081 ctctgccttg ccggctttac tttccccggc cgggatggtc aactgtttag ccggtggcgt
     1141 agccggtgct tcagcgatct tggtcacccg ttggacggag ttcttcgtca ggatatttcc
     1201 gatggttccg cgtcctttga cctcaaactg attgaagtct acatcaagtt gggtgatgcg
     1261 cagtttcggt ttaggacgca ggattacacg cacggtctct ccgctttcgt tcggcatcgc
     1321 ggacaggtag aggatcttgg atccatcatt ccctttcgtc agcacgtatt ccttgtcacg
     1381 ggtggtaccg ccaaccgtga atcgtttcac catgatgttg ccgccccgtc catcctggta
     1441 aatgaggttg aacacaggtt caggctccga cttccggaac accgaaatgt agcgaacatc
     1501 cttcccgacg tatttcttgt cgtccacttt ggtgacgatc atcgtaccgt cgccgcggat
     1561 gaccaggatc tcgtcgaggt cggagcattc cgatacgaac tcgtccttct tcataccgaa
     1621 gcccgcaaaa ccgtcgtcgc ggttcacgta cagcttctgg ttgttgatga tcacttccga
     1681 agcgaccacc tgttcgaacg tgtcgatctt cgtcttccgt tcgcgaccct ttccgtactt
     1741 tttgagcagg tctttgaacc agttaatggt gtattgccgg atgtgcgcga ggtggtgctc
     1801 cacctcttcg agttgggcct cgaggccgcg gatcttttcg tcctgctcct tcacatcgta
     1861 cttcgagatc cgtttgatct tgatctcggt tagcgaaatg atatcgtcac gggtgatcgc
     1921 gcgtttgaat ttcttcttgt acggcttgag tccctcgtcg atcgtctcga taacgctctc
     1981 ccaggtcgta cattcttcaa tgttacggta gatgcgtttc tcgatgaaga tcttgaccag
     2041 cgaactgaag tgccactcct cttccaactc cgacttccgt atctccagct cccgcttgag
     2101 gaggttgagc gtctggtcgg tattgtgacg aaggatttcg ttgacggtaa ggaacttcgg
     2161 tttatcgtcg acgatcacgc aggcattagg tgaaatgctg atctcgcagt cggtgaacgc
     2221 gtaaagcgcg tcaatggtct tatcaagatc ctccatcgag tcgctatgca acgtaagcac
     2281 gatctcgaca ttggacgctg tgttatcctc gactttcttg atccggatct tgcctttgtc
     2341 gttggcggcc aggatactat cgatcaggct cgtcgtggta gtaccgaacg ggatctcccg
     2401 gatgatgagt gtcttgcggt ccttcttctc gatgcgtgcg cgaatgcgga tccggccacc
     2461 gcgcaagcct tcgttatatt tcgaaaaatc agcgatgccg cccgtcggaa aatccggcag
     2521 gatgttgact ttcttgcccc gcaagaggtc gatcgacgct tccagcaact caacgaagtt
     2581 gtgcggcagg atgcggcagg cgagacctac cgcgatgcct tccacgccct gtgcgagcaa
     2641 caatgggaac
//
LOCUS       DAOTON010000374         2649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30181_length_2649_cov_2.871627, whole genome shotgun sequence.
ACCESSION   DAOTON010000374 DAOTON010000000
VERSION     DAOTON010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30181_length_2649_cov_2.871627"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="P5292_07925"
     CDS             complement(<1..437)
                     /locus_tag="P5292_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRS39094.1"
                     /translation="MKKILSITLLLFRVVAAVAQTPLSFKDPLTGMYGFKDAQGKVII
                     PAVFRAVTQFNSDLSLAQEPTGGIAIPIKPSGFPLGNDYHIADAQKVLLGGLYGEHGG
                     KTMEPKNPEAIPSTYNSILFSYVEPAKRMSVVKNSALSVNKNA"
     gene            complement(637..>2649)
                     /gene="metG"
                     /locus_tag="P5292_07930"
     CDS             complement(637..>2649)
                     /gene="metG"
                     /locus_tag="P5292_07930"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRS39095.1"
                     /translation="GPVHIGHLAGAYLPADVYVRYLRAMGEDVVFICGSDEHGVPITL
                     RARKEGVSPQQVVDKYHGIIRQSFADFGISFDIYDRTSSPIHHETASDFFKTLFEKGE
                     FTEESSEQYYDEEAGLFLADRYITGTCPNCGNTNAYGDQCEKCGKSLSPRELINPHSQ
                     LSGKPPVLRQTKHWYLPLDRYQREWLEKWIDGHRDDWKPNVFGQCKSWLDQGLQPRAV
                     TRDLDWGVPVPLPDSKGKVLYVWFDAPIGYISATKALFRQRGDANGWEKYWKSKDARL
                     VHFIGKDNIVFHCIIFPAMLHAHGGYVLPENVPANEFLNLEGDKISTSRNWAVWLHEY
                     LQDFPGKQDVLRYALCATAPETKDNDFTWKDFQARNNSELVAILGNFVNRTLVLTAKY
                     YNGKVPAAHALSDEDRACLEAIQAAPGKLGDLIRRYKFREALFELMNLARLGNKYLAD
                     NEPWKKQATDPQRVETILHVSLRITAALARLMEPFLPFTSEKLHGMLNLVHAKWTAAW
                     DETLLPVGHQLGEAGLLFEKIEDPAIETQVARLQASKAANEAASAAAPAVSPQKAPTS
                     FDDFQKMDIRIGTILAAERVPKTEKLLKLTVDTGIDQRTVVSGIAAHYDPEKIVGQQV
                     TILVNLEPRKIKGIESQGMILMAENAKGELAFLQPTKAVENGGTIS"
BASE COUNT          522 a          725 c          790 g          612 t
ORIGIN      
        1 ttcgcgttct tgttgaccga tagtgctgaa tttttgacaa cggacattct cttagcgggt
       61 tccacatagc taaaaagaat ggagttatag gttgacggga tggcttccgg gtttttcggt
      121 tccatcgtct tgcctccgtg ttctccatat agcccaccaa gcagtacttt ctgagcatct
      181 gctatatggt aatcgttgcc taaagggaag ccactgggtt taatcgggat ggcgattcct
      241 ccggttggtt cctgcgcaag ggacaggtcg ctgttaaact gggtgactgc cctgaacaca
      301 gcaggtatga ttactttgcc ctgagcgtct ttaaaaccat acatgcccgt caacggatcc
      361 ttgaaagata gcggcgtttg ggcaacagcg gcgacaactc tgaaaagcaa gagggtgatg
      421 gagagaattt ttttcataac agcaacgggc tctcgtgtaa tggggatgga tgtaaatata
      481 tggaatccga tattctgggg ataggatgcg ttccacctcg acggtgcctg gtcgtatcat
      541 cgaccataga gagtacaaca agacgaggtg gttaaaccgg tagaaataac gactcgcgac
      601 tgcccgtacg aataagttga tacgatccgt cggggatcag gagatcgtac cgccgttctc
      661 caccgctttc gtcggttgca ggaacgccag ttctcctttc gcgttctcgg ccatcaggat
      721 catgccctgc gattcgatgc ccttgatctt gcgcggctca aggttcacga ggatggtcac
      781 ctgctgcccc acgatctttt ccggatcgta gtgtgcggca atgccggaaa ccaccgtacg
      841 ctgatcgatg ccggtgtcca cggtcagctt cagcagcttc tccgtcttgg gaacgcgctc
      901 cgcggcgaga atggtgccga tgcggatgtc catcttctgg aagtcatcga aggaggtcgg
      961 cgctttctgc ggactcacgg ccggtgccgc tgcactggcg gcttcattcg ccgccttgct
     1021 ggcctgcaga cgtgctacct gcgtttcgat ggcggggtct tcgatcttct cgaacaaaag
     1081 gccggcttcg ccgagttggt gaccaacagg caagagggtc tcgtcccagg cagccgtcca
     1141 tttcgcatgc acgaggttca gcatgccgtg cagtttctca ctggtaaacg gcaggaaggg
     1201 ctccatcaga cgcgcgaggg cggccgtgat gcgcagggag acgtgcagaa tggtttccac
     1261 acgttgcgga tcggtcgcct gcttcttcca gggttcgttg tcggccaggt acttgttgcc
     1321 gagtcgcgcg aggttcatca gctcgaacag ggcttcccgg aatttgtagc gacggatgag
     1381 atcgcccagt ttgcccggcg ctgcctgaat ggcttcgagg caggcgcggt cttcatcgct
     1441 gagcgcgtgc gcagcaggga cttttccgtt gtaatacttg gctgtcagca ccagcgtgcg
     1501 gttcacgaag ttgccgagga tggctaccag ctcgctgttg ttgcgtgcct gaaaatcttt
     1561 ccaggtgaaa tcgttgtctt tcgtctccgg cgccgtggca caaagcgcgt agcgcagcac
     1621 gtcctgtttg ccggggaagt cctgcagata ctcgtgcagc cacaccgccc agttgcggga
     1681 ggtggagatc ttgtcacctt cgaggttcag gaactcattg gccggaacgt tctccggcaa
     1741 tacataacca ccatgcgcgt gcagcatggc cgggaagatg atgcaatgga aaacgatgtt
     1801 gtccttgccg atgaaatgca cgagccgcgc gtctttcgac ttccagtact tttcccaacc
     1861 gttcgcgtca ccgcgctggc ggaagagcgc ctttgtcgcg gagatgtagc cgatcggcgc
     1921 gtcgaaccag acgtacagca ctttgccttt gctgtcgggc agcggaacgg gaacgcccca
     1981 gtcgaggtcg cgggtcaccg cgcgcggttg cagcccctgg tcgagccagg acttgcactg
     2041 accgaatacg ttcggcttcc agtcgtcgcg gtggccgtcg atccattttt ccagccattc
     2101 gcgctggtag cggtcgaggg gcaggtacca gtgtttggtc tgacgcaaca ccggcggctt
     2161 gccgctgagc tgcgaatgcg ggttgatcag ctcacggggc gaaaggctct tgccgcattt
     2221 ctcgcactgg tcaccgtagg cattggtgtt gccgcaattc ggacaggtgc cggtgatgta
     2281 acggtcggcc aggaagaggc cggcttcttc gtcgtagtat tgttcgcttg actcttcggt
     2341 gaattcacct ttctcgaaaa gtgttttgaa aaaatccgaa gccgtctcgt ggtgaatagg
     2401 agacgaggta cggtcataga tatcgaacga aatgccgaaa tcggcaaagc tctgccggat
     2461 gatgccgtgg tacttgtcca ccacctgttg cggcgagacg ccttccttcc gcgcacgcag
     2521 ggtgatcggt acgccgtgct cgtcggaccc gcagatgaac accacatcct cgcccatggc
     2581 ccgcaggtaa cgcacataca cgtcggcagg caaataagcc cccgccaggt gcccgatgtg
     2641 aacaggacc
//
LOCUS       DAOTON010000375         2648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30189_length_2648_cov_3.971076, whole genome shotgun sequence.
ACCESSION   DAOTON010000375 DAOTON010000000
VERSION     DAOTON010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30189_length_2648_cov_3.971076"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="P5292_07935"
     CDS             complement(<1..207)
                     /locus_tag="P5292_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39096.1"
                     /translation="MTPPLTTTLTAPEVQVVAQPEPKRAVVNAVLRCLLYFDIFHHPL
                     DAKEIQECLSVPCDTGALEETLSYL"
     gene            complement(325..1443)
                     /locus_tag="P5292_07940"
     CDS             complement(325..1443)
                     /locus_tag="P5292_07940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HRS39097.1"
                     /translation="MVEQHACDSLYHNDWFQESLRKHGFTEQGKFSPFPPPTTYLFLP
                     LTPLPPLVAKRVWMVISLLLVAWIIWLFGRISGMNGRDATAWVLATGLALINNLYLGQ
                     VYLLMVAFMLYGWIQFRKGKTIVPGICFGIVAAIKYFPLVMLPEWLLKRRWKLTLSFA
                     LTITTLGLVSLYSPGVPACRDFLNRVVFAHLDGRLEGQSPFATAFQSWNALSHRLFLF
                     DAAENPQPWFHAPMLLQLVRHGIPALLLFFGIVHARRLILRNASGAGVAILCLSMLVA
                     SPASASYHLLLLLFPMALLLEDETGRSRMSPASTRLSFLLLMGIGWAPLFIGKFLAPA
                     TWPIPFQFYRLWLMLVIWLVAMRLTSHRFQVNPDRFIL"
     gene            1713..>2648
                     /locus_tag="P5292_07945"
     CDS             1713..>2648
                     /locus_tag="P5292_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRS39098.1"
                     /translation="MAKDRFQYHDYYQVDELLSEEHKLVRDSVREWVKKEVSPIVEDY
                     CQRAEFPRQWIKGLGEIGAFGPYIPTEYGGGGMDQIAYGLIMMELERGDSGLRSTASV
                     QSSLVMYPIFTYGSEEQKRKYLPKLATGEMMGCFGLTEPDHGSNPAGMITNIKDKGDH
                     YLLNGAKMWISNAPFADIAVVWAKDEEGVIRGLIVERGMAGFSTPETHGKWSLRASAT
                     GELVFDNVKVPKENLLPNVKGLKGPLGCLNSARYGIAWGAIGAALDCYDSALRYSKER
                     IQFGRPIGGFQLTQKKLAEMITEITKAQLLTWRLGV"
BASE COUNT          714 a          709 c          727 g          498 t
ORIGIN      
        1 taaatacgac aaggtctctt ccaaagcgcc ggtatcgcat ggaacggaga ggcactcctg
       61 aatctccttc gcgtccagcg ggtgatgaaa aatatcgaag taaaggaggc accgcagaac
      121 ggcgttgacc accgcccgct taggttcggg ttgggcgaca acttgtactt ccggcgccgt
      181 aagcgtggtg gtcagaggag gggtcacaag tgttaaggtg aatgtttcaa agctactaat
      241 cttcccgcaa gggaattgtt gccagcctgc gaattatgca cgcccatgac gattcccaca
      301 caaaatcatg acgaacgggt aaaatcagag gatgaaacgg tcaggattga cctggaagcg
      361 atgacttgtc agtcgcatgg cgaccagcca gatgaccagc atcaaccaca atcgatagaa
      421 ttgaaacggt atcggccagg tagcgggcgc taagaatttt ccaatgaaaa gcggcgccca
      481 accgattccc atcaggagga gaaatgaaag tcgggtggaa gcaggactca tccgggatct
      541 gccggtttca tcttccagga gcaaagccat tggaaagagt aaaagcagga gatggtaaga
      601 cgccgacgca ggtgaagcaa ccaacatact caggcaaagg atcgccacac ctgctccgct
      661 ggcatttcgc agaatcaatc gccttgcgtg aacaatcccg aagaataata gcaatgccgg
      721 aattccatgt cggaccagtt gcaacaacat tggcgcgtgg aaccagggtt gtgggttttc
      781 tgcagcatcg aacaggaata agcgatggga aagagcgttc caggattgga aagcagtcgc
      841 gaagggtgat tgcccttcca gtcgtccgtc cagatgcgcg aaaacgaccc ggttcaggaa
      901 gtcccgacag gctggtacac ccggactgta aagcgaaacc aaacccagcg tggtgatggt
      961 aagtgcgaat gacagggtga gtttccaacg tcgcttcagc agccattcag gtaacatgac
     1021 gagcgggaaa tacttgatcg cggcgacaat gccgaagcat atgcccggta cgatcgtttt
     1081 ccctttccgg aattgaatcc acccatacag catgaaggct accatcagga gatacacttg
     1141 tccgaggtaa aggttgttga tcagtgccag gcccgttgcc aacacccagg cggtggcatc
     1201 ccgcccattc atcccggaaa tccttccaaa gagccaaatg atccaagcta ccagaagcag
     1261 actgatgacc atccacacgc gtttggccac aaggggtggg agcggagtca acggcaaaaa
     1321 caaataggtg gtcggcggag gaaacggaga aaacttgcct tgctctgtaa agccatgttt
     1381 ccgtaaggac tcctgaaacc aatcattgtg ataaaggctg tcgcaagcat gctgctccac
     1441 cataagtcgt gagccgagcc aataattcgg aaagtcactt tgcatcctct gccagcctgg
     1501 tcgtacaccg gcaaaaaaaa tcaaaccacc agcgatacat aagaacatgt atttcagcaa
     1561 attgacttta ccggacaaga gtcggggcag tggcatcgca acaaagaaac aaatttgact
     1621 gttccagcca ttcaaggggt ttctcccacg gaactccgta aattcgcagc cccgtccgaa
     1681 gcaggacgta ttcaaagatc accctgtcaa tcatggccaa agaccgtttt cagtaccacg
     1741 attattacca agtcgatgaa ctcctgagtg aagaacacaa actcgtacgc gattctgttc
     1801 gcgaatgggt gaaaaaggag gtttcgccga tcgtcgaaga ctattgtcaa cgcgcggaat
     1861 tcccccggca atggatcaaa ggcctcggag agatcggtgc cttcggtcca tatataccaa
     1921 ccgaatacgg cggcggcggc atggaccaga tcgcgtacgg actcatcatg atggaactgg
     1981 agcgcggcga ttccggtctg cgttcgaccg cgtcggtgca aagctcgctg gtcatgtacc
     2041 cgatcttcac ttacggcagc gaagagcaga agcgcaagta cctccccaaa ctggcgaccg
     2101 gtgagatgat gggttgcttc ggtctgacgg aaccggacca cggctccaac cctgccggca
     2161 tgatcaccaa catcaaggac aagggcgatc actatctcct caacggagcg aaaatgtgga
     2221 tcagcaacgc gcccttcgcg gatatcgcgg tcgtgtgggc gaaggatgaa gagggtgtca
     2281 tacgcggact gatcgtcgag cgcggcatgg ccgggttctc cactcccgaa acgcacggta
     2341 aatggtcgct gcgcgcaagc gccaccggcg aactggtctt cgataacgta aaggtgccca
     2401 aggaaaacct cctgccgaat gtcaaaggcc tgaaaggtcc gctcggctgc ctcaactccg
     2461 cccgctacgg catcgcctgg ggcgcgatcg gcgcggcgct cgactgctat gattccgcgc
     2521 tgcgctactc gaaagaacgc atccagttcg gcaggccgat cggcggtttc cagctgacac
     2581 agaagaaact tgccgagatg atcaccgaga tcaccaaggc gcaactgctc acctggcgcc
     2641 tcggggta
//
LOCUS       DAOTON010000376         2648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30192_length_2648_cov_3.341304, whole genome shotgun sequence.
ACCESSION   DAOTON010000376 DAOTON010000000
VERSION     DAOTON010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30192_length_2648_cov_3.341304"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="P5292_07950"
     CDS             complement(<1..703)
                     /locus_tag="P5292_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HRS39099.1"
                     /translation="MIASNQSVSKQTGWPIGVWYAELTHPYWAFSEAGYTVDIATPEG
                     GALFFDSFSDPEDASGYSAFDYISLGFKKDSARMAMTRNTKKLSDVNPADYKAIFVCG
                     GQGPMYTFYKNPQVEKFFTDFYQTGKPTAAICHGTCLLLEAKLPNGKYLVEGKRWTGF
                     ASEEEQYADNYVGKQIQPFRIEDLARRMPNSRYEDAAPFSAFAVRDGNLITGQQQNSG
                     AKAAELVLQALEEDRK"
     gene            931..1317
                     /locus_tag="P5292_07955"
     CDS             931..1317
                     /locus_tag="P5292_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRS39100.1"
                     /translation="MPDFVYDHKVYYNPVEFALLFIGGTWKMPILWRLNQATMRYGEL
                     KKALPHITHKMLSSQLRELEQHGLVARKVFAQVPPKVEYSITAKGKRTIPLITAIRKY
                     GLEMMKEKGIDTSKDGASGKKKNRKG"
     gene            complement(1476..>2648)
                     /locus_tag="P5292_07960"
     CDS             complement(1476..>2648)
                     /locus_tag="P5292_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRS39101.1"
                     /translation="LDEAERYSRQAMSIHQLLNEKDGLSQDYYNLCAIYVMQKDFPKA
                     ISFGRDALRLSQELGYPQNIRNSAQSLVSAYKGAGMYPQAFDMLYLYTRMNDSINNRE
                     LRKNLIRRQLQYDYDRKALADSIRNVAERQLVTERLRKERAQRLALWGGLIMTLVFGG
                     FIYNRFLVTRRQKIQISQEKQRSDELLLNILPEEVAEELKTKGSADARLFDDVTVMFT
                     DFKNFTLISERLSPTELVNEIHTCFKAFDQIISKHNIEKIKTIGDAYMCAGGLPIANK
                     TNAMDVVNAALEIQKFMAELSSERKAAGREVFEIRIGIHTGPVVAGIVGVKKFAYDIW
                     GDTVNIAARMESSGEVGKVNISENTYALVRHRFKCIHRGMIEAKHKGQINMYFVEE"
BASE COUNT          594 a          717 c          672 g          665 t
ORIGIN      
        1 gcttgcggtc ttcttccagt gcctgcaaca ccagttcggc tgctttggct ccgctgttct
       61 gttgctgtcc ggtgatcagg ttgccgtcgc gcacagcaaa ggcggagaac ggcgccgcgt
      121 cttcgtatcg gctgttcggc atgcggcgtg ccaggtcttc gatgcggaac ggctggatct
      181 gcttaccgac gtagttgtcg gcatactgct cctcttcgga agcgaaaccg gtccagcgct
      241 taccttccac caggtacttg ccgttgggaa gtttcgcttc cagcagcagg caggtgccgt
      301 gacagatggc ggcggtgggc tttcccgttt ggtagaaatc ggtgaagaac ttttccacct
      361 gcggattctt gtaaaaagtg tacatcggtc cctgaccgcc gcagacgaag atggccttgt
      421 agtcggccgg gttcacgtcc gacagctttt tcgtattccg ggtcatggcc atcctcgccg
      481 agtctttctt gaagccgagc gagatgtaat cgaaggcaga ataaccgctg gcgtcttcgg
      541 ggtcgctgaa actgtcgaag aaaagagcgc cgccttcggg cgtggcgatg tcgacggtgt
      601 aacccgcttc ggagaatgcc cagtacgggt gcgtgagttc cgcgtaccag accccgatgg
      661 gccagccggt ctgtttgctg acactctggt tggaagcgat catgaggatc ttgccgttgt
      721 ggccggcatt gtgttgcgcg taggccgccg tcagggcaag caggatggcg caggagagga
      781 ggaggcgttt gatcatggtg ttcgtcgttg ttcggggaca aaattcggca cagcaggatc
      841 cacttcgcct attactacct gaaagtataa tactatcttt atggaaagta ttggatccta
      901 ccccgtccga cagctctact tttatccgcc atgcccgatt tcgtttacga ccacaaagtc
      961 tattacaacc cggtggaatt cgccttgctc ttcatcggtg gcacctggaa gatgccgatc
     1021 ctctggcgcc tgaaccaggc gacgatgcgt tacggcgaac tgaaaaaagc gctgccccac
     1081 atcacccaca agatgttgtc gtcgcaacta cgcgaacttg aacagcacgg actggtcgcc
     1141 cgaaaagtct ttgcccaggt acccccgaaa gtcgagtaca gcatcaccgc caagggcaag
     1201 cgtacgatcc ccctgatcac ggcgatccgt aagtatgggc tggagatgat gaaggagaag
     1261 ggtattgata cttcgaagga tggcgcttcc ggcaaaaaga aaaatcggaa gggttgagga
     1321 atccggttga taacaacctg acacacgacc tcgtttagcc gcagcaatgg tgcggccggg
     1381 aaacacttta gcaccgggtc atcttgtccc ttctaatgaa tcattcccgg ttcggagggg
     1441 atagcggaag tcaggggcca ccaggctccc ttgatctact cctctacaaa gtacatattg
     1501 atctgccctt tatgcttcgc ttcaatcatt ccgcgatgga tacacttgaa tctgtgcctc
     1561 accaatgcat acgtgttctc gctgatattc accttaccga cctcaccgga cgattccatt
     1621 cgggccgcga tgttcacggt atcgccccag atatcatacg cgaatttttt cacgccaacg
     1681 attcccgcca cgacaggacc ggtatggatg ccgattcgta tttcgaacac ttctcttccg
     1741 gcagctttcc gttcagaaga caactcggcc atgaactttt gaatttcaag agcggcattc
     1801 acaacatcca tggcattggt cttattggca atcggtaaac ctccggcaca catataggca
     1861 tccccgatcg tcttgatctt ctcaatatta tgcttggaga tgatctgatc gaatgcctta
     1921 aaacaggtgt ggatctcgtt taccagctct gtcggactca aacgttcgga aatgagtgtg
     1981 aaatttttaa aatccgtgaa catcaccgtt acatcatcga acaaacgcgc atcggcactt
     2041 cctttggttt tcaactcctc cgctacttct tcaggcaaaa tattcaagag taattcgtca
     2101 cttctctgct tttcctgact gatctggatc ttctgtcgac gcgtgacgag gaagcgatta
     2161 tagatgaagc ctccgaatac aagggtcatt attaatccac cccaaagtgc cagtcgctgt
     2221 gcccgctcct tacggagccg ttcggtgacg agttgtcgtt cggccacatt ccgaatgctg
     2281 tctgccagcg ctttccggtc gtaatcgtat tgcaactgac gtctgatcag attcttccgc
     2341 agctcccgat tattgatgct gtcattcatc cgtgtatata aatacagcat gtcaaacgcc
     2401 tgtggataca tgccagctcc tttgtaggcc gacacaaggc tttgcgccga attgcgaatg
     2461 ttctgcggat accccaattc ctgactcaat ctcagggcgt cccgcccgaa ggaaatcgcc
     2521 ttaggaaaat ctttctgcat aacatagatg gcacacagat tataatagtc ttgagataag
     2581 ccgtcctttt cattcaacaa ttggtgaatg gacatagctt gccgtgagta acgctcagcc
     2641 tcatccag
//
LOCUS       DAOTON010000377         2644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30254_length_2644_cov_3.461568, whole genome shotgun sequence.
ACCESSION   DAOTON010000377 DAOTON010000000
VERSION     DAOTON010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30254_length_2644_cov_3.461568"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="P5292_07965"
     CDS             <1..617
                     /locus_tag="P5292_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS39102.1"
                     /translation="GKSSVINLIGRQYEFQRGEIRIDGSDVRSFSLDSLRRNIAMVMQ
                     DVFLFSDTIHNNITLYDPSISREQVEEAAKRVGAHRFIERLPGGYDFNVMERGAMLSV
                     GQRQLLSFIRAYVFNPRILILDEATSSIDAESEALIQQATEVLTSNRTSIVVAHRLAT
                     IQKADKIVVLDHGRKMEEGTHAELMRRDGIYRKLYEVQFSDILQ"
     gene            747..1517
                     /locus_tag="P5292_07970"
     CDS             747..1517
                     /locus_tag="P5292_07970"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRS39103.1"
                     /translation="MKTKIILLIALCLGAFVQVKAQTADEIVDKYVAATGGADRWSKL
                     KTVRMSGSLDLAPGMKAPFTIAMMDRTKMRFELEIQGMKMIQALDGDSGWKVMPFQGK
                     TDPERMSEEEIRSSKEQADYTGDLYDYKAKGSVVELLGKEDMEGTETFKLKVTKKNGD
                     VKYMYIDATSFLALKETSKQKFNDKEVEMSTINSNFQTVDGITLPFSIEMRGGDDQQG
                     GGQAMTFDKIEINPVIDAKLFVMPAMSSATAPAAGETK"
     gene            1600..>2644
                     /locus_tag="P5292_07975"
     CDS             1600..>2644
                     /locus_tag="P5292_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRS39104.1"
                     /translation="MKRIITMLALVTGYLSAGAQNAPKVTSSLFGMMEARSIGPAVMG
                     GRITSIDANVKDPRIVYVGTAGGGIWKTINGGASFKSVFDKYCQSIGALTIDQGHPET
                     IWAGTGESNMRNSVSIGNGIYLTTDGGDNWTRMGLDSTEHIARILIHPRDPKTVYVAA
                     PGPLWSDSPHRGLYKTSDGGKTWEKILYTDVKTGCAEVVMDPSNPDVLYASMWQFRRK
                     PFAFASGGPGSALYKSSDGGKTWKKLTKGLPTGEIGRVALALAPSASSNLFAIVEAKK
                     TSLYLSTDSGESWQEQSSNFNVEARPFYFSTIAVDPANPKRVYRPAFSMSFSDDGGRS
                     WSDPANTSGWVHSD"
BASE COUNT          647 a          693 c          722 g          582 t
ORIGIN      
        1 cgggcaagtc ctcggtcatc aacctgatcg gtcgccaata cgagttccag cgcggcgaga
       61 tccggatcga tgggtcggat gtccgatctt tttccctcga cagtctgcgc cgtaacatcg
      121 ccatggtcat gcaggatgtt ttcctgtttt cggacacgat ccacaacaac attaccctct
      181 atgacccatc catcagcagg gagcaggtgg aagaggctgc caaaagagtg ggcgcacatc
      241 gtttcatcga acggctcccg ggaggatacg acttcaacgt catggagcgc ggagccatgc
      301 tatcggtcgg ccagcgacag ttattatcgt tcatccgggc gtatgtgttc aatcccagga
      361 tcctgatcct cgacgaggcg accagttcca tcgatgctga gtcggaggcc ctgatccaac
      421 aggctactga agtgctgacc agcaatcgaa cgtcgatcgt ggtggcacat cggttggcga
      481 ctattcagaa ggctgataag atcgtggtgc tggatcatgg ccgcaagatg gaggaaggga
      541 cgcatgcgga actgatgcgg cgcgatggaa tataccgcaa gctgtatgaa gttcagtttt
      601 ccgatatttt acaataacct tccggctatt tgactgttat ggttgggatc ggtttgcggt
      661 tggtcgcaac ttctgtacct taacctttca ttaacctccg gctgccggac aattcactta
      721 gtttgtaacc atcatccatt caacccatga aaacaaaaat cattcttctc atcgccctct
      781 gcctcggagc attcgttcaa gtaaaggcac aaacggccga tgagatcgtt gacaagtacg
      841 tagccgccac cggtggcgct gaccgttggt ccaaattgaa aaccgtacgc atgagcggaa
      901 gccttgacct ggcgcccggt atgaaagctc cctttaccat cgccatgatg gaccggacca
      961 aaatgcggtt cgaactggaa atccagggaa tgaagatgat ccaggcgctg gatggtgatt
     1021 ccggttggaa ggtcatgccg ttccagggta agaccgatcc cgagcgcatg tcggaggaag
     1081 aaattcgctc ttccaaagag caggccgact ataccggtga cctctatgat tacaaggcca
     1141 aagggagtgt agtggaactg ctcggcaagg aagacatgga aggtaccgag acctttaaac
     1201 tcaaagtcac caagaagaac ggcgacgtaa aatacatgta catcgatgcg acctctttcc
     1261 tggccttgaa agaaaccagc aaacagaaat tcaatgataa agaagtggag atgtctacca
     1321 tcaactccaa cttccagacg gtcgatggca tcacgcttcc gttctccatt gaaatgcgtg
     1381 gcggcgatga ccagcagggt ggaggtcagg cgatgacgtt cgacaagatt gagatcaatc
     1441 cggtcattga tgccaagctg tttgtcatgc cggccatgag ttcggcgacg gcaccggctg
     1501 ccggcgaaac gaagtaattc aacaaccaca ttaggggagg gcattttcag gctgcttgtt
     1561 tgctgaagat gccctttctg tttacctaaa atttctgcca tgaaacgaat cattaccatg
     1621 cttgctctgg taactggata cctgagcgca ggcgcgcaaa acgctccgaa agtaaccagt
     1681 agccttttcg gtatgatgga ggcacgatcg atcggtcccg ccgtgatggg cggacgtatc
     1741 acttccatcg acgccaatgt caaagacccg aggatcgttt acgtgggtac ggccggtggc
     1801 ggaatctgga agacgatcaa tggaggggct tcattcaaat ctgtgttcga taaatattgt
     1861 cagagcattg gcgcgctgac catcgatcag gggcatccgg aaaccatctg ggccggcacc
     1921 ggagagtcca acatgcgtaa cagcgtatcg atcggaaacg gaatttatct gacgaccgat
     1981 ggaggcgata actggacccg gatggggctc gactcgaccg agcacattgc ccggatcctg
     2041 atccatcccc gcgatccgaa aaccgtctat gtggcagctc ccggtccgct ctggagcgac
     2101 agtccgcatc gcggtctcta caagacatcg gatggcggaa aaacatggga aaagatcctc
     2161 tacacggatg tgaaaaccgg ttgtgccgag gtagtcatgg atccttcgaa tccggatgtg
     2221 ctgtatgcca gcatgtggca attccgtcgc aaaccattcg cattcgcttc cggcggtccc
     2281 ggcagtgcgc tctataaaag ttccgatggc ggtaagacct ggaaaaagct gaccaagggt
     2341 ctgccgaccg gtgagatcgg acgcgtagca ttggcactgg ctcccagcgc gtcctcgaat
     2401 ctctttgcca tcgtagaagc gaagaagacg agcttgtacc tctctacaga ttccggtgag
     2461 tcgtggcagg agcaaagttc gaacttcaat gtggaagccc gacccttcta tttttcgacc
     2521 attgccgtcg atccggctaa tcccaagcgg gtttatcgcc cggccttctc catgagtttt
     2581 tccgacgatg gaggacgttc ctggtccgac cctgccaaca ccagcggatg ggtgcacagc
     2641 gacc
//
LOCUS       DAOTON010000378         2628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30514_length_2628_cov_3.748543, whole genome shotgun sequence.
ACCESSION   DAOTON010000378 DAOTON010000000
VERSION     DAOTON010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30514_length_2628_cov_3.748543"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="P5292_07980"
     CDS             complement(<1..538)
                     /locus_tag="P5292_07980"
                     /inference="COORDINATES: protein motif:HMM:NF040245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39105.1"
                     /translation="MSKTSTSTLQLAPVYRETFDRLFSGEKDNTWLPMRRSAMQSFEA
                     IGFPGPRTEAWRYTNLAKVTAMPFVPAPSTEQLQALELEKLEAVRLDGIRLVFENGRY
                     RESLSSLQSLPSVAIVGSLARHHDHPKVKEYLQQNLPLEHEAFVALNTAFMPDGAFVF
                     IPRGVQIEQPIHLVFVNDA"
     gene            complement(543..1301)
                     /gene="sufC"
                     /locus_tag="P5292_07985"
     CDS             complement(543..1301)
                     /gene="sufC"
                     /locus_tag="P5292_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRS39106.1"
                     /translation="MLTIKNLHASIEGKEILRGLSLEVKPGEVHAIMGPNGSGKSTLA
                     SVLAGREEYEVTGGSVSLKGENLLELSPEDRARKGVFLAFQYPVEIPGVSNANFLKTA
                     VNEVRKARGQNLLDSKDFIALMKEKMALVEMDKAMTQRSVNEGFSGGEKKRNEIFQMA
                     MLEPSLCLLDETDSGLDIDALRIVANGVNALRSPERSFVVITHYQRLLDYIVPDVVHV
                     LYKGRIVKSGPKELALELEAKGYDWIKEEVSAGV"
     gene            complement(1347..1799)
                     /locus_tag="P5292_07990"
     CDS             complement(1347..1799)
                     /locus_tag="P5292_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRS39107.1"
                     /translation="MATIERFEDLKVWQEARVLANLTYRVSSLSNFQKDFVLRDQVRR
                     AVVSILSNIAEGFERNSPKEFVHFLSMAKGSAGELRAQLYLSFDQNYIDKKEFKELYD
                     KVTTISKMLSGLMSYLQNANLRKKNYQVTEPEALYQRIIKQRIKVNNP"
     gene            complement(1904..>2628)
                     /locus_tag="P5292_07995"
     CDS             complement(1904..>2628)
                     /locus_tag="P5292_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRS39108.1"
                     /translation="PMRDENQLHAAVVEIYAHRDAQVKYSTVQNWYPGDKEGRGGIYN
                     FVTKRGLCAEANAKISWTQVETGSAITWKYPSVILKGDNSIGEFYSVAVTNNYQQADT
                     GTKMIHLGKNTRSTIVSKGISGGRSQNSYRGLVKISKKADNARNFSQCDSLLLGDRCG
                     AHTFPYLEVHNKNAKVEHEATTSKVGEDQIFYCNQRGISTEDAVSLIVNGYCKEVFNQ
                     LPMEFAVEAQKLLAISLEGSVG"
BASE COUNT          552 a          709 c          671 g          696 t
ORIGIN      
        1 gcgcatcgtt cacgaaaacg aggtggatcg gttgttcgat ctgcacgccc cgtggtatga
       61 atacgaatgc cccgtccggc atgaaggcgg tattgagcgc gacgaatgcc tcgtgttcaa
      121 gcggaaggtt ctgttggaga tattccttca ctttcgggtg atcgtggtga cgagcaagcg
      181 accctacgat agcgacggaa ggcaaggact gtaaactgct gagtgattcg cggtaacgac
      241 cgttctcgaa aaccagccgg atgccgtcga gccgtaccgc ttccagtttt tccaattcca
      301 atgcctgcaa ttgctcggtg gaaggcgcag gcacaaaggg catggccgtc accttggcaa
      361 ggttggtata acgccaggct tccgtacggg gaccgggaaa gccgatcgct tcgaacgact
      421 gcatcgccga acggcgcatg ggcaaccagg tattgtcctt ctcaccggaa aataggcggt
      481 cgaacgtctc ccggtaaacc ggtgcaagct gcagcgtgga tgttgatgtc ttgctcatgg
      541 gatcagactc ctgccgacac ttcttctttg atccagtcgt atcccttggc ttcgagctca
      601 agggccagtt ccttcggacc cgattttaca atgcggcctt tgtacaggac atgcacgacg
      661 tcgggcacga tataatccag caatcgctgg taatgggtaa tgactacaaa ggatcgctcg
      721 ggcgaacgca gggcgttcac gccgttggcg acgatccgca gggcgtcgat gtcgagcccg
      781 gaatccgttt cgtcgagcag gcacagcgaa ggttccagca tggccatctg gaaaatctcg
      841 ttacgcttct tttctccgcc gctgaagccc tcgttcacgg accgttgggt catcgccttg
      901 tccatttcaa caagggccat cttctccttc atcagggcga tgaagtcctt ggagtcgagc
      961 aggttttgtc cgcgcgcttt ccgtacttca ttcacagcgg ttttcaggaa attcgcgttg
     1021 ctgacacccg gtatctcgac aggatactgg aatgcgagga agacgccctt gcgcgcacgg
     1081 tcctccggag aaagttccag caggttctcg cccttcagac tgaccgagcc gccggtcact
     1141 tcatactctt cgcgtcccgc caatacggaa gcaagtgtac tcttaccgga accattcggc
     1201 cccatgattg catggacttc tcccggcttg acttccaaag aaagtccgcg cagaatctct
     1261 tttccttcga tggaagcgtg gaggtttttt atcgttagca tctctaatga tgttcgttta
     1321 aggttgaatg ttggcaagtt gaaagatcac ggattattga ccttgattct ttgcttgatg
     1381 atcctttgat atagtgcttc aggttcagtt acctggtagt tcttctttcg aaggttggcg
     1441 ttttgaagat agctcatcaa accggaaagc atcttgctta tcgtggtgac tttatcgtat
     1501 agttccttga attctttttt atcgatgtaa ttctggtcga atgacaaata aagttgtgct
     1561 ctgagttctc cagcacttcc tttcgccatg gaaagaaaat ggacgaactc tttcgggcta
     1621 ttgcgctcga acccctctgc tatgtttgac agaatggaga caactgctcg tctgacctga
     1681 tcccgcagaa caaaatcttt ttggaaattt gataaagagg ataccctata agtcaggtta
     1741 gccaacacgc gtgcttcctg ccaaactttt agatcctcaa accgctcaat agtagccatt
     1801 ccaactttct actttctact ttcaactttc agctttcaac cttcaacttt caacgtgaaa
     1861 acgttcaaac gttataacgg tataacgttc taacgccccc tcatcacccc accgacccct
     1921 ccaacgatat cgccagcaac ttctgggctt ctacggcgaa ctccatcgga agctggttga
     1981 atacttcttt gcaatagccg ttcacgatga ggctgacggc atcttcggtg gaaatcccgc
     2041 gttggttaca atagaagatc tggtcctcgc cgactttgga ggtcgtcgcc tcgtgctcga
     2101 ctttcgcatt cttgttgtgc acttcgaggt acgggaaggt gtgcgcaccg catcgatcgc
     2161 caagtaatag cgaatcgcat tgggagaagt tccgggcgtt gtcggctttc ttgctgatct
     2221 tcaccagtcc ccgatagctg ttctgactgc gcccgcctga gattcctttt gacacgatcg
     2281 tgcttcgggt attcttcccg aggtggatca tcttcgtgcc ggtatcggct tgctggtagt
     2341 tgttcgttac ggcaacggaa taaaattctc cgatgctgtt gtcgcctttg aggatgacgc
     2401 ttgggtactt ccacgtgatc gcggagccgg tctccacctg cgtccaggat atcttggcat
     2461 tcgcctccgc acacagaccg cgcttggtga cgaaattgta gatacctcct cgcccttctt
     2521 tgtcgcccgg gtaccagttc tggacggtgg aatatttcac ctgcgcgtcc cggtgcgcgt
     2581 agatctcgac caccgccgcg tgcaactggt tctcgtcgcg catgggcg
//
LOCUS       DAOTON010000379         2625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30566_length_2625_cov_2.963424, whole genome shotgun sequence.
ACCESSION   DAOTON010000379 DAOTON010000000
VERSION     DAOTON010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30566_length_2625_cov_2.963424"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="P5292_08000"
     CDS             complement(<1..585)
                     /locus_tag="P5292_08000"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRS39109.1"
                     /translation="MNSIRDRLYSRLQQPLPGIESQLRLAHPDRIKNVRELKIPDNAR
                     ISSVLVLLYPGDAEVHFPLIVRPDYPGVHSGQVGLPGGKQEPEDSSLSATALRETHEE
                     VGIHPDTIELLGRLTPLYIPPSGFLVHPFVGWLDRSPAFAPDPAEVASLFEVSVSRLL
                     DPNAVSEERIRLQSGLQLTTPAFRFDDRIVWGATA"
     gene            complement(582..2384)
                     /locus_tag="P5292_08005"
     CDS             complement(582..2384)
                     /locus_tag="P5292_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS39110.1"
                     /translation="MKLLFSYLSKHRKTVFLALLLAAVNQIFSLMDPLIAGKMMDRFG
                     MHISDYRGDPISRFVGDILLLLLASMGVAMVSRIAKNFQDYFTSVIVQRTGAAMYTDG
                     IRHSLELPYQVFEDQRSGETLGKLQKVRLDSEKLINSFISVVFQTLVGLIFVLWYAFT
                     VHWIIAPVFLVTVPLLGVISNLLSKRIKKVSKEIVSETTALAGSTTESLRNIELVKSL
                     GLANQEVTRLNTITGKILKLELKKVRFIRSLSFVQGTTVNFLRTSFVFLLYYLVFRDT
                     ISPGKLMTLMFFSFFIFGPLQELGNVIAIYRETQVSLQNFQTILNTPKEPRPAHPATI
                     GPVERLRYDRVTFRHQASTHNAVENISFEVTKGQSVAFVGPSGSGKTTLVKLLLGLYP
                     PYSGDIYYNDIPGSQIEKDELREQIGFVSQDTQLFSGTIRENLLFVRPGATDEDCYRV
                     LRQAACQSLLDRASNGLDTVIGEGGVKVSGGEKQRLSIARALLRNPKLLIFDEATSAL
                     DSITEEEITGTIRQLSARQEHITIMIAHRLSTILHADRIIVLEKGQVVETGTHQELIE
                     TKGLYYAMWRQQIGERKRDEERSNGSARPAPQHA"
BASE COUNT          610 a          667 c          766 g          582 t
ORIGIN      
        1 cgccgtcgca ccccagacaa tacggtcatc gaagcggaag gccggcgtgg tgagttgcaa
       61 accgctttgc aaccggatgc gctcctcgga aacggcattg ggatctaaga gccgggatac
      121 ggaaacttca aataaggaag cgacttcggc tgggtcggga gcgaatgcag gtgatcggtc
      181 cagccagccc acgaaggggt gcaccaggaa gccgctggga ggaatgtaga gtggcgtcaa
      241 tcggccaagc aattcgatcg tatcgggatg aatgccgact tcctcatggg tctcccgcag
      301 agcggttgcg gataaactgc tgtcttccgg ttcctgcttc ccgccgggta aaccgacctg
      361 tccactgtgt acacccggat aatcgggacg aacgatcagg gggaaatgga cttcggcatc
      421 gccgggatag agcagcacca agacgctgct gattctagcg ttgtccggaa tcttcagttc
      481 gcgaacattc ttgatgcggt ccgggtgtgc aaggcgaagt tgtgactcga taccgggtaa
      541 gggttgctgc agccgtgaat agagacgatc ccggatactg ttcatgcgtg ctggggcgcc
      601 ggacgtgcgg atccattcga tcgttcttca tcacgcttgc gttcaccgat ctgttgacgc
      661 cacatggcat aataaagtcc tttggtttcg atgagctcct gatgcgttcc ggtttccacc
      721 acttgtccct tttcgagtac gatgatgcgg tccgcgtgca ggatcgtact cagccggtga
      781 gcgatcatga tcgtgatatg ctcctgccgg gcggaaagtt gtcgtatcgt accggtaatt
      841 tcttcttcgg taatggagtc cagcgccgac gtggcttcat cgaagatcag gagctttgga
      901 ttacgcaaca gcgctcttgc aatcgaaagg cgctgcttct cgccgcccga aacttttacg
      961 cctccctcgc caatgacggt gtccagcccg ttggaagcgc gatccaacag ggactgacag
     1021 gcggcctggc gtagaacacg atagcagtct tcatccgtgg cgcccgggcg gacgaacaac
     1081 aggttctccc ggatggttcc cgagaagagt tgcgtatcct gtgatacgaa accgatctgt
     1141 tcgcgcaact cgtccttttc gatctggctt ccggggatgt cgttgtaata gatatcaccg
     1201 gagtaaggag ggtagaggcc gaggaggagt ttcaccaggg tggtcttgcc ggagccggaa
     1261 gggccgacga atgcgacgct ttgacctttg gtcacctcaa aggagatgtt ctccaccgcg
     1321 ttgtgcgtcg aggcctgatg acgaaacgtg acgcgatcat agcgtagacg ttcgaccggc
     1381 ccgatcgtgg cgggatgtgc cggtcgcggt tccttcggtg tgttcaggat ggtctgaaag
     1441 ttctgtaagg aaacttgtgt ctcacggtag atggcaatca cattcccgag ttcctgtaac
     1501 ggtccgaaga tgaagaagct gaagaacatc agggtcataa gctttcccgg tgagatcgtg
     1561 tcgcggaaga caagatagta gagtagaaat acgaaggagg ttcgcaggaa gttgacggtc
     1621 gttccctgca cgaaactcag actgcgaatg aaacgcacct tcttcagctc cagcttcagg
     1681 atctttccgg tgatcgtatt caaccgggtc acctcctggt tcgccagccc gaggcttttt
     1741 accagttcga tattccgtaa ggactcggtc gttgagccgg caagggcggt cgtttcagag
     1801 acgatctcct tcgaaacttt cttaatccgt ttactgagca ggttgctgat gacgccaagc
     1861 aaaggaacgg taacgaggaa aaccggcgcg atgatccagt gtaccgtgaa ggcataccat
     1921 aagacgaaga tcagtcccac cagtgtctgg aaaactacac tgatgaacga attgatcagt
     1981 ttctcactgt ccaaccggac tttctggagc ttgcccagcg tttcgccgct gcgctggtct
     2041 tcaaagacct ggtaagggag ttcgagcgaa tgccggatcc cgtcggtgta catcgccgcg
     2101 ccagtccgct gcacgattac actggtaaag tagtcctgga agttcttcgc tattctggat
     2161 accatggcaa cacccatcga ggcaagcaac aacagcagga tgtcgccgac aaaccggctg
     2221 atgggatcgc cgcgataatc actaatgtgc attccgaacc ggtccatcat ctttccggcg
     2281 atgagcgggt ccattaggga gaaaatctgg ttgactgccg ctaacagcaa tgccaggaaa
     2341 acagttttac gatgtttgct gaggtaggaa aaaagcagtt tcatgtctgg ggaggaacgg
     2401 taaaggtaac gattgccctg ccctcatcat gaaggcttcg ttggaaaatg gtaataaaaa
     2461 aacacccccg cagacgcagg ggtgttgaat accggtaagg aacgaattac tgaagaacga
     2521 tccgctcggt aacccggtcg gaaccgtttt ctactacgag gttgtagagg ccggcaggca
     2581 ggtaagagaa gtcaagcgtc tgattgaaca gcccggaaac ctgtt
//
LOCUS       DAOTON010000380         2624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30581_length_2624_cov_3.952900, whole genome shotgun sequence.
ACCESSION   DAOTON010000380 DAOTON010000000
VERSION     DAOTON010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30581_length_2624_cov_3.952900"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="P5292_08010"
     CDS             <1..263
                     /locus_tag="P5292_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRS39111.1"
                     /translation="KQILEPILGIQDLRTDKRIDFVGGLRGLGELERRVNSGEMKVAF
                     ALYPVTMKQLIDISDSGKIMPPKTTWFEPKLRSGLILHSLED"
     gene            267..734
                     /locus_tag="P5292_08015"
     CDS             267..734
                     /locus_tag="P5292_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HRS39112.1"
                     /translation="MSSNQNLFHPPDFDSILQRIQNLNADAERKWGTMNVHQMVVHCS
                     DPIREVLGIRPTRNLSNLLLRTVGKWMALYGPEWQKGKFPTSPDYDQLKKGTPPTTFE
                     SDREQLQRLLEKVRELPLNHPMPPHPAFGKMSRKEWGRLGYRHLDHHLRQFNR"
     gene            743..1429
                     /locus_tag="P5292_08020"
     CDS             743..1429
                     /locus_tag="P5292_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRS39113.1"
                     /translation="MSPLPLVLLHGALGDVRQLQPLAKLLDDRTVISVNFPGHGTDAV
                     HSTPFTMDDLAAAITQVMDEHQVERADMFGYSMGGYAALWLLHLQPDRVRRLFTLGTK
                     LSWNPEIATREQANLQPEKIREKVPALMRSLAQRHGEEHWEKVVRSTAAMMQWLGSHP
                     LKEEDFLFMTHEICLGLGDADKMVSLEETQRVLGYLNNGSLSSLAGTPHPLEQVDLTL
                     LRDVLIRFLN"
     gene            complement(1435..>2624)
                     /locus_tag="P5292_08025"
     CDS             complement(1435..>2624)
                     /locus_tag="P5292_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HRS39114.1"
                     /translation="CLPTGEPYVSCDSEHFYTERTTLADHFSGKKTYLLESFYRMLRR
                     KHGVLMAGDEPEGSKWNFDQENRSRYDGKVSVPTELQLKHDVTDLYATVVQAGVRTMG
                     RVDPKALPWPKNRKESLRLLHWFCEQLLPFFGTYQDAMKSGEPFMFHSRLSFSLNTKM
                     LSPREVVDAAVAAYSDRPDLISLPQVEGFVRQILGWREYVRGVYWANMPGYAERNFFS
                     HVRELPGWYWNGETGMNCLHQTIRGSLDNAYAHHIQRLMITGNFALLAGVDPRAVDDW
                     YLGIYIDAIEWVEITNTRGMSQFADGGLVGTKPYVSSGNYIDKMSDYCSGCDYDRKSR
                     HGDKACPFNSLYWHFHQINRPLLEKNPRIGMVYRTWDKMSATERDKTLRQAEYYMENL
                     DML"
BASE COUNT          634 a          742 c          643 g          605 t
ORIGIN      
        1 cgaaacagat cctggaaccg attcttggca tccaggatct tcggacggac aaacggatcg
       61 actttgtggg cggtcttcgc ggccttggtg aattggaacg ccgcgtgaac agcggagaaa
      121 tgaaagttgc cttcgctttg tacccggtta cgatgaagca actcatcgat atttccgaca
      181 gcggtaaaat catgcctccg aagaccactt ggtttgaacc gaaactgcgc agtggattga
      241 ttcttcactc cctggaagat tgatccatgt cgtcgaatca aaacctcttt cacccacccg
      301 atttcgattc catcctgcag cgaatccaga atctgaatgc agatgccgag cgaaaatggg
      361 gcactatgaa cgtccaccaa atggtcgtcc attgcagcga cccgatccgg gaggtattag
      421 gaattcgacc aacacgcaat ctgagcaatt tgcttttgcg gaccgtcgga aaatggatgg
      481 cgctttacgg acccgaatgg caaaaaggga aattcccaac ttctcccgac tatgaccaat
      541 tgaagaaggg aacgccccca accaccttcg aatcggatcg tgagcaactt cagcgattac
      601 tggaaaaagt tcgcgaactt ccgctaaacc atccgatgcc gccccatccg gcttttggga
      661 agatgagcag aaaagaatgg ggtcggttgg ggtatcgaca ccttgaccat caccttcgac
      721 aattcaatcg ctgaggtttc ctatgtcccc cctgccgtta gtcttattac acggagcctt
      781 aggcgatgtc cgccaattgc aaccgctggc taaattgctg gatgaccgta ctgtcatctc
      841 cgtcaacttt cccgggcacg gaacagatgc tgtgcattcc accccgttca cgatggatga
      901 cctggctgct gccatcactc aggtgatgga tgaacatcag gtcgaacggg cagacatgtt
      961 cggttacagc atgggcggtt acgcggccct ttggttgctg cacttgcaac ctgaccgcgt
     1021 ccgccgcctt ttcaccctgg gaaccaagct ttcctggaat cctgaaatcg ccacccggga
     1081 acaggcgaat cttcagccgg agaaaatccg ggaaaaagtg cccgctttaa tgcgttcact
     1141 cgcccagcgt catggcgagg agcattggga aaaagtcgtt cgatccaccg ccgccatgat
     1201 gcaatggttg ggaagtcacc cgttgaaaga ggaagacttc ctcttcatga cgcacgaaat
     1261 ctgcctcggt ctgggtgatg cggataaaat ggtttcgctg gaagagaccc aacgcgtgct
     1321 gggctacctg aacaacggtt cactttcttc gctcgccggg acgcctcatc ctttggaaca
     1381 agtcgatctg acgcttcttc gcgatgtgct gatccggttc ctgaactaag acgttcaaag
     1441 catgtccagg ttctccatgt aatattccgc ctggcgcagt gttttatccc gttccgttgc
     1501 cgacatctta tcccaggtac gatagaccat cccgatgcgc gggttcttct ccagcagcgg
     1561 gcgattgatc tgatgaaaat gccaatagag ggaattgaac gggcaggcct tgtcaccatg
     1621 ccgtgacttc cgatcgtaat cgcaacccga acaataatca ctcatcttat cgatatagtt
     1681 acccgaggag acatatggct ttgttccaac cagccctcca tccgcaaatt ggctcatgcc
     1741 gcgggtgttc gtgatctcca cccattcgat cgcgtcgatg taaatgccca ggtaccaatc
     1801 gtcaacagca cgtggatcga cacctgccag caaggcaaag ttgccggtga tcatcaaccg
     1861 ttgaatatga tgggcgtaag cgttgtccag gcttccccgg atggtttgat gaaggcaatt
     1921 cattccggtt tcaccgttcc aataccaacc gggcaattcc ctgacatggg aaaagaaatt
     1981 tcgctccgca tacccgggca tattcgccca ataaactccc cggacatact cacgccaccc
     2041 gaggatctgt cgcacgaatc cttcgacctg tggaagcgat atcaagtcgg ggcgatcgct
     2101 gtaggcagcc accgcggcat ccaccacctc ccggggtgag agcattttcg tattcaggga
     2161 aaaggacaag cggctgtgga acatgaatgg ctccccggat ttcatcgcgt cctgataggt
     2221 gccaaaaaat ggaagcagtt gttcgcaaaa ccagtgcaac aaccgaagcg actccttcct
     2281 gttcttcggc catggtaatg ctttcgggtc gacgcgtccc atcgtcctaa ccccggcctg
     2341 caccacagtc gcatacaaat ccgtcacgtc atgcttcaac tgtagttccg ttggaactga
     2401 gactttcccg tcataccggc tacgattttc ctgatcaaaa ttccacttcg atccttcggg
     2461 ttcatctcca gccatcaaca ccccatgttt cctgcgcaac atgcgataaa agctttccag
     2521 gaggtaggtc ttcttccctg aaaaatgatc ggccagcgta gtccgttcgg tataaaaatg
     2581 ctcgctgtca caggagacat acggttcacc cgtcggaaga caag
//
LOCUS       DAOTON010000381         2620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30632_length_2620_cov_3.012476, whole genome shotgun sequence.
ACCESSION   DAOTON010000381 DAOTON010000000
VERSION     DAOTON010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30632_length_2620_cov_3.012476"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="P5292_08030"
     CDS             complement(<1..1515)
                     /locus_tag="P5292_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39115.1"
                     /translation="MTRPYILRLLLLLLNIFGITSIANAQPKSGGASYAALWQKVDSL
                     EGLGLTESARIVTESIRDKARQESQSAELVKAVVYRMKFIDYREEDALAKNLSTIQEE
                     IDAAVFPERPLLFSMKAELLWRYYQENRYRILDRGISAQDSTADPAQWDVARFVRETH
                     AAYQASLINAADQKRVQVNSFDPVLLPGNTAEARRFRPSLYDFLAHRAIDFYKQEESG
                     LTRPIQSFSINSAFYLNEADLFLNFLLKTEDTLSFDFQAIRLLQDLLDFHQDDTHPGA
                     YIDLDLERLRFVYDRLTIANRDTLYLKAVQRIADRYRNFPGSSDALFAIADWYASRAC
                     DRNESNTDSCAAYRKRAFAYCEEGISRFPYSQGGLNAKALRAQLLAPDLGLTLEKTQL
                     PARPILSSLNYRNVDSVWYKIVRLDDAFFESLQRENERQTEEKRLRRYLRQPVVEEGV
                     WVLPSSTDLNNHRIEEAFPALQLGRYAVLLATTPGFSTDRQAVVYADFQVSRITL"
     gene            complement(1639..>2620)
                     /locus_tag="P5292_08035"
     CDS             complement(1639..>2620)
                     /locus_tag="P5292_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39116.1"
                     /translation="PVQANVLAIPDPPIVADITRCGPGTVTLTAVSGSQVSWYNQASG
                     GTLLGTGSTFTTPVLTTTTLYYAEAGVDCRSIRVDVSAVISTVSTPPVVTPAGSCEPA
                     SLLLTASSSDPITWYDQASGGSVVGTGSSFQTPFLNSTTTYYAVAGVSCPSTAVPVDA
                     SIYTPPVISLGADTVTIQSGSTTVLDPGAGFADYSWSTTETTQTIEVGITGWYTVLVT
                     DGNGCEATDSIFVNVITGILPVGGGVEALSVFPNPAHELLLVRWEKRQAGEVLIELCD
                     GTGKLIRKFREEMSPGSQQVRFGMQDLAAGFYLLRISSDGDQQTVPVIRQ"
BASE COUNT          616 a          722 c          709 g          573 t
ORIGIN      
        1 taacgtaatc cggcttacct gaaaatcggc gtaaacgacc gcctgacgat ccgtcgagaa
       61 gccaggggtg gtagcgagta acactgcata ccttcccaac tgaagtgctg gaaatgcctc
      121 ctcgatccga tgattgttga gatcggtaga cgaaggaagt acccagacac cttcctctac
      181 aaccggctgc cgcaggtagc gtcgcaggcg tttttcttcg gtctgacgtt cgttctcgcg
      241 ttgcagggat tcgaaaaatg catcatccag gcgaacgatc ttataccaga cggaatccac
      301 attgcggtaa ttcaacgaac tcaggatcgg tcgtgctggc aattgggtct tctccaatgt
      361 caacccgaga tccggtgcaa gaagctgggc gcgtaaggcc ttcgcgttaa gccctccctg
      421 cgaatagggg aatcgactga tcccctcttc acaataggca aatgcccgct ttcgataggc
      481 cgcgcaactg tcggtgttgc tctcattgcg gtcgcaagca cgactcgcat accaatcggc
      541 aatcgcgaaa agcgcgtccg aggatccggg aaaattgcgg tagcggtccg cgatacgttg
      601 aaccgccttc aggtacaagg tgtcccgatt cgcaatggtc aggcggtcgt aaacaaaccg
      661 gagtctttcc aggtccagat cgatatatgc acccggatgc gtatcgtcct gatgaaaatc
      721 cagcaggtcc tgtaaaaggc gaatagcttg aaaatcgaaa ctcagcgtgt cttccgtctt
      781 gaggaggaaa ttgagaaaaa ggtccgcttc attcagatag aatgcgctgt tgatggaaaa
      841 gctttgtatg ggacgcgtca gaccgctctc ttcctgttta tagaaatcga tcgctcggtg
      901 agcaaggaaa tcatagagac tcggccggaa tcgtcgggct tcggcagtgt tgcctggaag
      961 caggaccgga tcgaagctgt tgacctgaac acgcttctga tccgccgcat tgatcaggga
     1021 tgcctggtag gccgcatggg tctcccgtac aaaacgggcg acgtcccatt gtgccgggtc
     1081 tgccgtactg tcttgtgcac tgatgccgcg atcgaggatg cgatagcggt tttcctggta
     1141 gtaacgccac agcaactccg ccttcatact gaacaacagc gggcgctccg gaaaaacagc
     1201 ggcatcgatc tcttcctgaa tggtcgatag gtttttcgcc aatgcgtctt cctcgcggta
     1261 atcgatgaat ttcatccggt aaaccaccgc cttcaccaat tcggccgact ggctctcctg
     1321 ccgcgctttg tctcggatcg actctgtgac gatccgggcg gattccgtca gacccaagcc
     1381 ttcaagtgaa tccacctttt gccataaggc cgcatagctt gctcccccgg atttgggttg
     1441 ggcatttgca atggaggtaa taccgaaaat attcagcagc aataacagaa gccggaggat
     1501 gtagggacgc gtcatatcgg taggattggc aataatagag accggagcgg gccggcttcc
     1561 acagcagcgc aaagttcact acaaaaaaac ggccgtgaca agtatcacgg ccgtttgagg
     1621 atcgattttg taaacacttt actgtcgtat gaccggaaca gtctgctggt caccatctga
     1681 tgatatacgc agcagataga atccggcggc aaggtcttgc ataccaaagc gaacttgctg
     1741 agatcccgga ctcatttcct cgcggaactt tcgaatcaat ttaccggttc cgtcgcacag
     1801 ttcgatcagg acttctcccg cctgccgctt ctcccaccga accaataaca gttcgtgtgc
     1861 cgggttgggg aatacgctga gggcttccac tccgccacca accggaagaa taccggttat
     1921 gacgttcacg aagatcgagt ccgtggcttc acaaccgttg ccatcggtga cgagcaccgt
     1981 ataccaaccg gttatgccga cttcgatcgt ctgtgttgtc tcggtggtag accatgaata
     2041 gtctgcaaat cctgcccccg gatcgagaac cgtcgttgat ccgctctgaa tagtcacggt
     2101 gtcagcaccg aggctgatta caggaggggt gtaaatgctg gcatcaacag gtaccgccgt
     2161 gctgggacag cttacaccgg ctacggcgta ataggtcgtc gtggagttca ggaatggcgt
     2221 ctggaagctg cttcccgttc ccactaccga acctccggat gcctgatcgt accaggtgat
     2281 cgggtcgctt gaagaagccg tcagcaagag cgaagcaggc tcgcaagaac cagccggggt
     2341 aacaaccggc ggggtggata cggtactaat cacagccgac acatccacgc ggatactgcg
     2401 gcaatcgaca cccgcctccg cataatacaa ggtagtagtc gtcagcaccg gtgttgtgaa
     2461 cgtactaccg gtacccagca gcgtacctcc gcttgcctgg ttgtaccagc tcacctgaga
     2521 tcccgagacg gcggtaagcg taaccgttcc cggtccacaa cgtgttatat ccgctacaat
     2581 gggcggatcg ggaatcgcca gcacattggc ctgcaccggc
//
LOCUS       DAOTON010000382         2614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30721_length_2614_cov_3.814771, whole genome shotgun sequence.
ACCESSION   DAOTON010000382 DAOTON010000000
VERSION     DAOTON010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30721_length_2614_cov_3.814771"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..700
                     /gene="ssrA"
                     /locus_tag="P5292_08040"
     tmRNA           333..700
                     /gene="ssrA"
                     /locus_tag="P5292_08040"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(863..1261)
                     /locus_tag="P5292_08045"
     CDS             complement(863..1261)
                     /locus_tag="P5292_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39117.1"
                     /translation="MRRFLVVFFLITVMFGSCTKDQGPLIIQPAPIPGDTMISFTRDI
                     QPLFDAMCIRCHNEWHPFLDLRTCCSFEQLLTSGHLAPYVDSLDPENSILIGRLRGVE
                     FPLMPPDGGRVSEGMIDTIKVWMLQGARPW"
     gene            complement(1338..2282)
                     /locus_tag="P5292_08050"
     CDS             complement(1338..2282)
                     /locus_tag="P5292_08050"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRS39118.1"
                     /translation="MKRQPFNDSLILRWLARCMLLLIVWLPDIPARAQLEVFDESPGD
                     KDMFRSSTLVNMQTVQTGVPGSWEFHIRHRFGYVKADQSLIRNFLGTDGVANIRFSFQ
                     FPLGSRGMLGIARTKSGKTYEMEGKYRFLDQSTDNRRPFALAVYVNVACMSDDFPPVS
                     QFAYFGDGRQRFTYELAHRFSYSSQLILARKFKERFRALLIPSFQYYNLVTPGYQNHR
                     LGLAAGGSVALSTKASVVAEYHYRFGRKPENGLFPATLGIEFGTVGHSFSVFVSSSQA
                     LNTQQLEWSTPYDYTNGEFLIGFNLRRTFWKKAKNEKG"
     gene            complement(2279..>2614)
                     /locus_tag="P5292_08055"
     CDS             complement(2279..>2614)
                     /locus_tag="P5292_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRS39119.1"
                     /translation="PQIPWVAPEAVNKVENALPHSAEVVSLGKKLYESTCWTCHGLDG
                     KGDGPAAATLRVKPADHTSLKIQSESDGAIFWKISTGRGDMQAYNKLFTVRERWALVH
                     YIRSLSSKR"
BASE COUNT          652 a          712 c          630 g          620 t
ORIGIN      
        1 gtgcgggtgc gaaagacgag ggacgtggga tgagcgacga gggacgtggt gcgaggtgcg
       61 aggtgcgagg tgcggaaaac gaggtaccgg aggttctttc agtaaagaat aatgtgcctt
      121 cgtatcacgg cttagcgaac cgttcgaacg atagggtttt gcattttcct acaagacaca
      181 accagtaacc attccaacca acgctgccaa ctgcctaccg catactgcct actgcctact
      241 gcctaccgcc taccgcctac cgcctacccc taccgccttc tgctccgccg ccaaccgcct
      301 catccacttt tcgtatcttt gcatctacct atggggctga ccggttttga cagctggatg
      361 aatcgttccg taagcatgcc gagcgtcgtt agtcaggctc gttaatcccg gctttcaaag
      421 attagttggc aacaccaact acgcgttggc tgcttaatca gaccgattaa ccagatccga
      481 ctgtttcctg gagacctgct cacccgtctc ctgtacggaa gcatcgcatg gtgagtgggc
      541 agacgcaagg ttccgtagcg gctgccgaga tcaacgggac taagcctgca ggtcgccatc
      601 gtccggcagc cggcgggtcg aaaatcaaag gccgataagc atgtagaaag cggggttatt
      661 cccggtttgg acgcgggttc gactcccgcc agctccacct ccatcaaaaa acggaagcag
      721 ccatcagcta cttccgtttt tttatgattc tattcgttga ccttccggca tcggctctct
      781 gccatgaacg cgaatggtta ccagcccgga accgcactgt ggttgattcg aaccaggcaa
      841 cgctacaata tcccgaacag gttcaccagg gtcgtgctcc ctgtaacatc catactttga
      901 ttgtatcgat catgccttcc gagacacgtc ctccatccgg cggcattagc ggaaattcca
      961 ctccgcgtaa gcggccgatc aaaatactgt tttcaggatc caaagaatcg acataaggcg
     1021 ccagatgccc ggaggtcaag agttgttcaa acgaacaaca cgtcctcaga tccaggaagg
     1081 gatgccattc attatgacac cgtatgcaca ttgcatcaaa caaaggttga atatcccggg
     1141 taaacgagat catcgtatca ccagggatcg gcgcaggttg tatgatcaga ggtccctggt
     1201 ccttggtgca agaaccgaac atgaccgtta tcaggaaaaa aaccaccagg aatctacgca
     1261 tggatccgct tggcatttgc atgacgtaat gctgaggtat gaccctgcag cgattttgac
     1321 gcttgttttt cattccacta gcccttctca tttttcgcct tcttccaaaa tgttctacgc
     1381 agattgaaac cgatcagaaa ttccccattg gtataatcat aaggtgtact ccattccagt
     1441 tgctgtgtgt tcagcgcctg cgaagacgac acgaagacgc taaatgaatg accgacggtg
     1501 ccgaattcaa ttcccagcgt cgcagggaac aaaccattct ccggctttcg gccgaaacgg
     1561 taatgatatt ccgctacaac agaggcttta gtagatagtg caaccgaacc accggctgca
     1621 agtccgagcc gatggttctg atagcccggt gtgaccaggt tgtaatattg aaaggacgga
     1681 atcaacaaag cccgaaagcg ctccttgaac tttcttgcaa ggatcagttg ggaagaatag
     1741 gaaaatcggt gcgccaattc ataggtgaat cgctgtcgac catcaccgaa atacgcgaat
     1801 tgggaaacag gcggaaagtc gtcgctcatg caggctacat tgacgtagac agccagcgcg
     1861 aagggtcttc ggttgtcggt cgattgatcc agaaaccggt acttcccttc catctcatag
     1921 gtttttccgg attttgttct tgctataccc agcatacccc gactgccaag cggaaattgg
     1981 aaactgaaac ggatattcgc cacaccgtcg gttcccagaa aattccggat caaggactgg
     2041 tctgccttta catacccgaa acggtgtcga atatgaaatt cccaggatcc agggactccc
     2101 gtttgcacag tttgcatatt gaccaatgtg gaagacctga acatatcttt atctcccggg
     2161 ctttcgtcaa atacttcgag ttgagcccgg gcggggatat ccggtaacca gacgatcagc
     2221 aacagcatgc atcgggcaag ccatctcagg atgagcgaat cattaaacgg ttgtcttttc
     2281 atctttttga agataacgag cggatatagt gtaccaatgc ccatcgctcc cggaccgtga
     2341 acaacttatt atatgcctgc atatcgcctc gcccagtcga tatcttccaa aaaattgcac
     2401 catcactttc cgattgaatc ttcaagctgg tatggtcagc cggcttgacc cgtagcgttg
     2461 ccgcagcagg tccgtcacct ttgccatcaa gtccatgaca tgtccaacag gtactttcat
     2521 ataacttctt tccaagactg accacttcgg cactgtgcgg cagggcgttc tccactttat
     2581 tcactgcttc cggcgctacc cagggaatct gcgg
//
LOCUS       DAOTON010000383         2611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30756_length_2611_cov_4.933099, whole genome shotgun sequence.
ACCESSION   DAOTON010000383 DAOTON010000000
VERSION     DAOTON010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30756_length_2611_cov_4.933099"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..349
                     /locus_tag="P5292_08060"
     tRNA            262..349
                     /locus_tag="P5292_08060"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:296..298,aa:Ser,seq:gga)
     gene            complement(748..>2611)
                     /locus_tag="P5292_08065"
     CDS             complement(748..>2611)
                     /locus_tag="P5292_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39120.1"
                     /translation="FTFFYFQFNLVSPAGDAGKFLDYPTLGVDASALYIGGVRFNPNV
                     FDGCPAFVVRKSSVLGAGPIVATAFRTAGGTSTGIYVPQGVHNDDPAATEGYFIGTDA
                     GVYGAINVIRIGTPGGTPTMTALPAITIPLNSNPQLQVHQGAAANRRLDALDDRLFAA
                     HIMQNKLTGASSLWTCHTSRANSSGVSSGTQNRNVTRWYQLTNLTTTPALAQSGTLYD
                     NAATNPRGFWNGSIAMTGQGHAVLGCTSAGAAARADVAVAGRYSSDASGTLQTFNLAT
                     NSSTTYVAQATDGQRWGDYSQTVVDPNDNMTAWTFQEYCDAANSWGLRVVQLKAPAPP
                     PTASLNALPTVGNLASVTVNVIANSTPNNTAFFDPGADAGGPGWANHITATATGGIAV
                     NGATFISPTQVNLDLNTTGVSAGTYTITIINPDGQTTTIPITISSTLPIELISFSARA
                     TENFCELDWVTASETNNDYFTIERSVTGEEFEAVAKVKGAGTTTTLENYEYRDMQPHE
                     GLSYYRLRQTDYDGTSTVSDIIPVDLKRSKFELTGISPDWNRQLVHVYFSDKNDQTLD
                     YVIVDQLGKTVLNGRIQSNRGSNALRIDASSLAKGMYYFNIGTGEKGLSGKFYY"
BASE COUNT          544 a          711 c          756 g          600 t
ORIGIN      
        1 caccaactca tcaacgccaa ccccgacaca ctcggcctcg gcccagaaca gttcatggtc
       61 ctcctcgcct cgatcgtcct aacggccttg ttctttgttt tgggtaaacg caatgcccaa
      121 tccgtgtagg tccggtttca tccgtgtgct ccgtgtgctt agaattcgaa gcaggccaaa
      181 gattgactta aagccccagg tttcgtacct tcgtatccct caaccgccaa aaactcacaa
      241 aaacgagttt tttcgcatcc cggagacgtg tccgagtggt tgaaggagca cgcctggaaa
      301 gtgtgtatac cccaaaaggg tatcgtgagt tcgaatctca ccgtctccgc aaagcaaaag
      361 ggactaagcc aatcaaggcc tggtcccttt tctttttccg cacaggagct tgaggtttag
      421 aaaagccgca agccgaatgt tcggtttatg ttgttcgttt cccttagaca gatttttcta
      481 acacaaatta ttctccaaag acttaccgca tataattggt tgagcaaata agaaggcctg
      541 gcaatatgcc ttgtgataac gacacaatac ccattctccg ctcggtttag tggaagctcg
      601 acacgcagaa gtcgaaagtc ggttcaaaga gcattctgac aaacattccg ctttgcaata
      661 ctgttcgctc ccgcctacgc taaagctacg gcgggcagga ttcactgttc actgtttact
      721 gtttactgtt cactattcac tcctcactca ataataaaac ttcccactca accccttctc
      781 ccccgtcccg atgttgaagt agtacattcc ctttgcaagc gaggaggcgt cgatgcggag
      841 cgcgttggag ccgcggttgg attggatgcg gccgttgagt acggtcttgc caagctgatc
      901 cacgatgacg taatcaagtg tctggtcgtt cttgtcggag aaatagacgt gtaccaattg
      961 ccggttccaa tccggtgaaa tgccggtgag ctcgaacttg ctgcgcttga ggtcaaccgg
     1021 gatgatgtcg gatacagtgg aggtaccgtc atagtcggtt tgacgcagtc gataatacga
     1081 gagtccttcg tgcggctgca tgtcgcggta ctcgtaattc tccagcgttg tggtcgtacc
     1141 cgcgcctttg accttcgcta cggcttcgaa ttcctctccg gtaacgctcc gctcgatggt
     1201 gaagtagtcg ttgttcgttt ccgaagcggt cacccagtcg agttcgcaaa aattttccgt
     1261 cgcacgtgcc gagaacgaaa tgagttcgat cggcagggtg ctggagatgg tgatcgggat
     1321 cgtggtggtt tgtccgtcgg ggttgatgat ggtgatcgta taggttccgg cagaaacacc
     1381 ggtcgtattg agatcgaggt tgacttgcgt cgggctgata aaggtcgccc cgttcacagc
     1441 gatgccgccg gtcgcggtag ccgtgatgtg gttcgcccaa cccggaccac cggcatcggc
     1501 gcccggatcg aagaaagcgg tattgttcgg ggtgctgttc gcgatcacgt tgaccgtgac
     1561 ggaagccagg ttgccgacag tcgggagcgc attcaggctg gcggtcggcg gaggagccgg
     1621 cgccttgagt tgaaccacgc gcaagcccca cgagttggcc gcgtcacagt attcctgaaa
     1681 cgtccaggcg gtcatgttat cgttcggatc gacaacggtc tgcgagtaat cgccccaacg
     1741 ctgtccgtcg gtcgcctgtg caacgtaagt cgtagaactg ttcgtggcga ggttaaaggt
     1801 ctgtaatgtt ccggaggcgt cggaagaata ccgccccgca accgcgacat ccgcgcgcgc
     1861 tgccgctccg gcggaagtgc atcccagtac cgcatggccc tgaccggtca tcgcgatgct
     1921 tccgttccag aagccgcgcg ggttggtcgc ggcgttgtcg tagagcgtgc ccgactgcgc
     1981 cagcgcgggc gtggtggtga ggttggtcag ttgataccag cgtgtcacgt tgcggttctg
     2041 agttcccgaa gaaacaccgg aactgttcgc ccgggaagta tgacaggtcc aaagggaact
     2101 cgcgccggtc agtttattct gcatgatgtg cgcggcgaag agtcgatcgt cgagcgcgtc
     2161 gaggcgacgg ttggccgccg cgccctggtg cacctgcagt tgcggattgc tgttcagcgg
     2221 aatcgtgatg gccggtaggg ccgtcatggt cggcgtaccg ccgggtgtac cgatccggat
     2281 cacgttgatc gcgccataga caccggcgtc ggtgccgata aagtaacctt ctgtggcggc
     2341 cggatcatcg ttgtgtacac cctgtggaac gtagataccg gtgctcgtcc ctcctgccgt
     2401 gcggaatgcc gtggcgacaa tcggtccggc acccagcacg gagctttttc tgaccacaaa
     2461 ggcaggacag ccgtcaaata cgttcgggtt gaatcgcacg ccaccgatgt aaagcgccga
     2521 ggcgtctaca cccagggtcg ggtagtcaag gaacttcccg gcatcaccgg ccggcgatac
     2581 gaggttgaat tgaaaataga agaaggtgaa c
//
LOCUS       DAOTON010000384         2604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30888_length_2604_cov_3.495488, whole genome shotgun sequence.
ACCESSION   DAOTON010000384 DAOTON010000000
VERSION     DAOTON010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30888_length_2604_cov_3.495488"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2266)
                     /locus_tag="P5292_08070"
     CDS             complement(<1..2266)
                     /locus_tag="P5292_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HRS39121.1"
                     /translation="MKETLTDSRVLCYHCGDECRETTLTFDSKTFCCDGCKLVYEILQ
                     ENNLCTYYQFNDKPGVSPKQARDERFAYLDDAQVRQKLIQFCDDSQTSVTFYIPQMHC
                     SSCIWLLEHLYRINQAVVRSRVNFLKREVSISFREKELSLRGLVEMLATIGYEPLIQL
                     NDLEKERKVKLDRSMAYRIGVAGFAFGNIMLFSFPEYFSLSDYIEPGFKGIFSYINFG
                     LSLPVFFYCSFPFFKAAWQSLHQRFLNIDVPIALGILIMFIRSLEEILTGIGPGYLDT
                     MAGLVFFMLIGRAFQNKTYATLSFERDYKSFFPIAVMVRKNDKETSIPVSSLQVGDRM
                     IVRNEELIPADSILLKGDASIDYSFVTGEAVPVTRRAGEWIYAGGKQQGAAIEVEVIK
                     PVSQSYLTQLWNNDAYLSKHEDSGFQLLVNRISHYFTIFILAVALVSLGYWMMTGDFN
                     RGWAAFTAVLIIACPCALAISSPFTLGNILRVFGRNKFYLKNYSVIEKLARVDTVVFD
                     KTGTITQRDQAETGWDGKTLDAEQLQAIRSLVMQSAHPLSRQVYRFLPELARLPVQEY
                     REVPGSGLSGIVSGMNILLGAPSFCGLEAVKTEETESSVYCNIDGKVLGRFFIRNAYR
                     EGLEDVLNYLNKHNFRRLVLTGDHPGEAVRLRQLFGADAEIRFRQSPADKLNAIQALQ
                     QEGRNVLMIGDGLNDAGALKQSDVGVSITDDINNFSPACDGILDGSRFNRLGSILRTA
                     KASRRIILGSFLIAL"
     gene            complement(2295..>2604)
                     /locus_tag="P5292_08075"
     CDS             complement(2295..>2604)
                     /locus_tag="P5292_08075"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRS39122.1"
                     /translation="VWSPEHLLVASISSCYLTTFNYFAQLLRVELKDIRVRGEVEIEK
                     VSGGGFWAKAYRIYPEIEFPNTPDPHVIDNLLSKAKRYCIVSNSVKGEVIVEPQIRYN
                     "
BASE COUNT          627 a          720 c          662 g          595 t
ORIGIN      
        1 ccagtgcgat cagaaaacta cccagaatga tccgacggct tgccttggct gtacgcagga
       61 tcgagcccag tcgattgaag cgggaaccat cgagaatacc gtcgcaggcg ggtgagaaat
      121 tgttgatgtc gtccgtgatc gataccccga catcgctttg tttcaaggca ccggcatcgt
      181 tcaacccgtc gccgatcatc aggacgtttc gtccttcctg ctgtaaagcc tggatcgcgt
      241 tgagtttgtc ggccggtgat tgacggaaac ggatctccgc atcggctccg aacaattgac
      301 gcaggcgcac ggcctctcca gggtgatcac cggtcagtac cagcctgcgg aagttgtgtt
      361 tattcaagta gttcaataca tcttccagtc cttcccgata cgcatttcgg ataaaaaacc
      421 ttccgaggac ttttccatcg atattgcaat acaccgacga ctccgtttcc tccgtcttga
      481 ccgcctccag tccgcagaac gacggagcac cgagcaggat attcattccg ctgacaattc
      541 ctgaaaggcc acttcccgga acctcccggt actcctgcac cggtaatcga gccaactccg
      601 gcaagaatcg gtacacttgt cggctcagcg gatgcgccga ttgcattacg agcgaacgga
      661 tcgcctgcag ttgttccgca tccaatgttt ttccatccca gcccgtctcc gcctgatcgc
      721 gttgcgtgat cgtgccggtc ttgtcgaaga cgaccgtatc cacccgtgcg agcttctcga
      781 tgaccgaata gttcttcaaa taaaacttat tccggccgaa cacccgtagg atgtttccga
      841 gcgtgaaggg cgaggagatg gcgagcgcgc acggacaagc gatgataagc accgcagtga
      901 aggcagccca gcctcgattg aaatcgcccg tcatcatcca atagccgagc gaaacaagcg
      961 ccaccgccaa tatgaagatg gtgaaataat ggctgatccg attgaccagt aactgaaaac
     1021 cggaatcctc atgtttgctg aggtacgcgt cgttgttcca caactgcgtc aggtagcttt
     1081 gcgaaaccgg cttgatcact tccacttcaa tcgcggcgcc ttgttgctta ccgccggcgt
     1141 agatccactc ccctgcccgg cgcgtaaccg gaaccgcttc accggtgaca aagctgtagt
     1201 caatgctcgc atcacccttc agtaagatgc tgtcggccgg aatcaattct tcgttccgta
     1261 cgatcatccg atcgccgacc tgcagtgagg agaccggaat ggaagtttcc ttgtcattct
     1321 tccggaccat gaccgcgatc gggaagaacg atttgtagtc gcgttcgaag gaaagtgtcg
     1381 cgtacgtctt attctgaaaa gcgcgaccaa tcagcatgaa gaagaccagg ccggccatcg
     1441 tatccagata tccgggaccg atgcccgtca ggatctcctc caggcttcga atgaacatga
     1501 tgagaatacc gagcgcgatc ggcacgtcga tgttcaggaa tctctggtgg agactttgcc
     1561 aagccgcctt aaagaacgga aaactgcagt agaaaaatac cggaagggaa agtccgaagt
     1621 tgatgtaact gaagataccc ttgaaacccg gctcgatgta atcgcttagc gagaaatact
     1681 cgggaaacga gaagagcatg atgtttccga acgcgaatcc cgctacgccg atccggtacg
     1741 ccatagagcg gtccagcttg actttccgtt ccttctccag gtcattcaat tggatcaagg
     1801 gttcgtagcc gatggtagcg agcatttcga ccagaccccg caggctcagt tccttctccc
     1861 ggaaactgat gctgacttcc cgtttcaaaa agttgacgcg tgaacggacg accgcctgat
     1921 tgatccggta aaggtgctcc aagagccaga tgcaactgct gcagtgcatc tggggaatgt
     1981 agaatgttac cgaagtctgg gaatcgtcgc agaactgaat gagtttctgc cgcacctggg
     2041 catcgtcgag gtaggcaaaa cgctcgtcgc gcgcctgctt gggagaaacg cctggtttat
     2101 cattgaattg gtaataggtg caaagattgt tctcctgaag aatctcgtac accaatttgc
     2161 aaccgtcgca acagaaggtc ttgctgtcga aggtgagtgt tgtttcacgg cattcgtcgc
     2221 cgcaatggta acacaagacc cgggagtcgg tcagtgtttc tttcattcta acaaccggcc
     2281 ttagcgaccg cgtttcaatt ataacgaatt tgcggctcta ctatcacttc gccctttacg
     2341 gaattgctga caatgcagta gcgcttcgct ttggaaagca ggttgtcgat tacatgcgga
     2401 tcgggggtat tcgggaattc gatctccgga tagatccggt aagcctttgc ccaaaatccg
     2461 ccacccgaca ctttttcaat ctctacctct ccccttaccc ggatatcctt cagttcaacc
     2521 cttaacaact gcgcgaagta gttaaacgtg gtgaggtaac acgaagagat cgatgcaacc
     2581 agcagatgtt ccggcgacca aacc
//
LOCUS       DAOTON010000385         2604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30895_length_2604_cov_2.668890, whole genome shotgun sequence.
ACCESSION   DAOTON010000385 DAOTON010000000
VERSION     DAOTON010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30895_length_2604_cov_2.668890"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="P5292_08080"
     CDS             complement(<1..498)
                     /locus_tag="P5292_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS39123.1"
                     /translation="MRPLNAQLPDTNATGGVYYKETGRETEVFRHCFREKLPLLLKGP
                     TGTGKSRYVEFMAAELQLPFLTVACHEETSTVDLLGRYIVKGDTTEWIDGPLTQAVRQ
                     GGIIYLDEIAEARPDVLVAIHSLTDHRRMLFLDRHHEVLRAPDDFMLVASFNPGYQRG
                     YKELKP"
     gene            complement(470..1810)
                     /locus_tag="P5292_08085"
     CDS             complement(470..1810)
                     /locus_tag="P5292_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRS39124.1"
                     /translation="MKYQSQKLAYWFFATCMLLLSLQLIYGFIMGFAHMGYDGLHSVI
                     PFNTARAVHTNLLVVWLLTGFMGAAYFIIPDEAGRELYSVKLGYIQLISLILVGVVAL
                     IGFHINWWEGRKFLEIPRPLDYLVVVNVLTFLFNIGATIYKSERISTTGIVLYTGLVA
                     AALLYLPGMIYFDNQTIDSYFRWWVVHLWVEGVWELIMGGILAYLLIKLTGVDREVIE
                     KWLYVIVGLTFLSGILGTGHHYYYIGTPKYWLIVGGIFSALEPLAFLGMALFAIVMYK
                     RSGRDYPNKLALLWTIGCAIMSFVGAGFLGFAHTLPQVNLYTHGTLVTAMHGHLAFWG
                     AYAMIVLAIIAYAMPQMTGRKRYSSMTGIIAFWMSNIGMLGMTGAFAVAGIAQVYLER
                     KSGMEFLAVQKEIEPHFFGLILAATLFTIAIILYIYEFIRYGRPTDEALERAAS"
     gene            complement(1844..2272)
                     /locus_tag="P5292_08090"
     CDS             complement(1844..2272)
                     /locus_tag="P5292_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRS39125.1"
                     /translation="MLSKSQARLFFIIGTVAFSGVFLWLTVDTMRKIPQQTRQQNITA
                     EVNRGKLIWDANNCMGCHTIFGEGAYYAPELTKAYERRGEQWLTLFLKDPEALYPGQR
                     KMVNYHFSDAEIADVIAFLKWVGEVDLNGFPAKPTLSQTK"
BASE COUNT          647 a          675 c          715 g          567 t
ORIGIN      
        1 gggtttgagt tccttgtatc cgcgttggta cccggggttg aaggacgcca cgagcatgaa
       61 atcgtccggg gcacgcaaca cttcatgatg acgatcgaga aagagcattc gacgatgatc
      121 ggtcagtgag tgaatggcca ccagaacatc ggggcgcgct tcggcgatct catccaggta
      181 gatgatcccg ccctgccgga ccgcctgcgt gagcggtcca tcgatccatt ccgtcgtatc
      241 tcccttcacg atataacgac ccagcaagtc gaccgtagag gtctcttcat gacaagctac
      301 cgtcaggaag ggtaattgca attcagcggc catgaactcc acatagcgcg acttacccgt
      361 tccggtaggc cctttcagta gcagcggcag cttttcccga aagcagtgtc ggaacacttc
      421 cgtttcgcga ccggtttctt tgtaataaac gccaccggtc gcgttggtat caggaagctg
      481 cgcgttcaag ggcctcatcg gtcggacgac cgtagcggat gaattcatag atgtagagaa
      541 tgatagcgat ggtaaagagc gtagcggcca ggatcaatcc aaagaaatgc ggctcaatct
      601 ccttttgaac agcgagaaat tccattccgg attttcgctc caggtacacc tgggcgatac
      661 ccgcgacggc gaatgctccg gtcattccca acatgccgat attcgacatc cagaaggcga
      721 tgataccggt catactggaa taacgtttcc gtccggtcat ctgcggcatt gcataggcga
      781 tgatggcgag gacgatcatc gcataagcgc cccagaatgc aaggtggccg tgcatggcag
      841 tgaccagggt cccgtgtgta tacaggttga cctgtggcag ggtgtgtgcg aagccgagga
      901 agccggctcc gacgaacgac atgatcgcgc agccgatggt ccacaataag gcgagtttgt
      961 tcggataatc gcgaccactg cgtttataca tcacgatggc gaagagcgcc attcccagga
     1021 aggccaacgg ctccaacgcg gagaagatgc cgccaacgat caaccaatac ttcggcgtgc
     1081 cgatataata atagtggtgg ccggttccga ggattcccga gagaaacgtc aagccgacaa
     1141 ttacatacaa ccatttctcg atcacttcgc ggtcaacgcc ggtgagtttg atgagtaggt
     1201 aagccaggat gccgcccatg atgagctccc atacgccttc cacccacaga tgcacgaccc
     1261 accaacggaa gtacgagtcg atggtctggt tgtcgaaata gatcatgccc gggagataca
     1321 agagcgcagc ggcaacaagc ccggtgtaca atacgatgcc ggtggtactg atgcgctccg
     1381 acttgtagat cgtggcgccg atgttgaaca ggaacgtgag tacattcacc acgaccaggt
     1441 agtcgagcgg ccggggaatc tccagaaact tcctgccttc ccaccaattg atgtgaaatc
     1501 cgatcagggc cacgaccccg acaaggatca ggctgatcag ttggatgtat cctagtttca
     1561 ccgagtaaag ttcacgcccc gcttcgtcgg gaatgatgaa ataggcagcg cccatgaatc
     1621 cggtgagtaa ccagaccacc agcaggttgg tgtggaccgc acgtgcggtg ttgaagggga
     1681 tgaccgaatg aaggccgtca taacccatgt gcgcgaagcc catgatgaaa ccgtaaatga
     1741 gctgcaggct cagcagcaac atacaggtgg cgaaaaacca gtacgccagt ttttgtgatt
     1801 ggtatttcat aggattgttt tcgttacagg ttgaacggat gggctacttc gtttgggaaa
     1861 gagtcggctt ggccggaaaa ccattcaggt cgacttctcc cacccatttg aggaatgcga
     1921 tcacgtcggc aatctccgcg tcgctaaaat gatagttgac cattttccgc tgtcccggat
     1981 acaatgcttc cgggtccttt aaaaaaagcg tgagccactg ctcgccccgc cgttcatagg
     2041 ctttcgtcag ttccggggcg taataagcgc cttcaccgaa gatggtgtgg cagcccatac
     2101 agttgttcgc gtcccagatc aatttaccgc ggttgacttc cgctgtgatg ttctgttgtc
     2161 gggtctgctg cggaattttc cgcatcgtat ccaccgtcag ccagaggaat acgcccgaga
     2221 atgccacggt tcctatgatg aagaataacc gggcttgtga tttggataac ataagcgtgt
     2281 gggttagaaa ttgtcgcgtt caactggttc cgaaaacttc ttcggaacgc gccctaagca
     2341 tggatagtaa aacatgggca tgatgctttg aaaagacaat caaaagtatc cctgcctggg
     2401 ggtgccggga atgatctccg atcctaaaaa aactatgaat cctatgtgga ttcagatgac
     2461 gattgtcagg attcctctac gtacgcacaa ttcaatggcc ggcaatttcc attacggtac
     2521 cagaagagaa agatgctggt gggggaccga tccatacgga ctgcggttca ggaagtgcca
     2581 aaaacaagcg ggtatccgct tgcc
//
LOCUS       DAOTON010000386         2600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30950_length_2600_cov_2.688802, whole genome shotgun sequence.
ACCESSION   DAOTON010000386 DAOTON010000000
VERSION     DAOTON010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30950_length_2600_cov_2.688802"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="P5292_08095"
     CDS             <1..307
                     /locus_tag="P5292_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRS39126.1"
                     /translation="EFLLINGVYQYNAWMAAVAGLTVILGAVYMLNAYRKISLGETGS
                     RTHSFDDLTLNEKAVLIPIVAVIFITGVYPKPLLDLVEPSVRALHDLVVQSGALSLR"
     gene            327..1697
                     /locus_tag="P5292_08100"
     CDS             327..1697
                     /locus_tag="P5292_08100"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRS39127.1"
                     /translation="MNTLIILAGLGLVLLLTEIFNIRKGQLALILIGLGAALAADIAD
                     WNAPVSLFNDMMRVDHFSVAFTGVILAVSLLWFTFSSSYFDEPSSRVDHFALLIFAIT
                     GAVVMTSFSNMIMLFLGIEILSISLYILAGSNKSNLASNEAALKYFLMGSFATGFLLF
                     GIALLYGATGSFNIAVIAEKIAANGLDQPVMITAGLFLMMIAMAFKVSAAPFHWWAPD
                     VYQGSPTVITAFMSTVVKTAAFAAFLRLFSGAFASIAINWSETLFIISALTIAIGNIT
                     AVFQTSFKRMLAYSSIAHAGYMTMALLSQQGQSGGSLLFYTAAYSVSSLAAFAILYLV
                     SNKTGNEQLEGFNGLASRNPFLAAITVVAMLSLAGIPPTAGFFAKYYIFTAAMEAGHT
                     WLVIIGVLGSLVGVYYYFRPLIALFRGTSDATIELPTATRIFLIGTAVLAIALGLAPG
                     YLSGLL"
     gene            1900..>2600
                     /locus_tag="P5292_08105"
     CDS             1900..>2600
                     /locus_tag="P5292_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRS39128.1"
                     /translation="MSNKQSKAGGIQGLFAALVIPLCLVLGIVIWNFIMGNPSGFENS
                     DPEKGPLPKEVIGTLNHYLAVMYKGGFVVPILLGSFFTVVVFAIERMITIGKSKGTGS
                     VEGFIMRIRPLVASGNLDGAKAECAKQRGSVANVVSAGIDMYRSMQADRELDKERKIM
                     AIQKELEEATTLELPMLSKNLVIISTIASIATLLGLFGTVLGMIRAFSALATAGAPDA
                     VALSTGISEALINTAL"
BASE COUNT          509 a          773 c          696 g          622 t
ORIGIN      
        1 cgaattcctg ctcatcaacg gcgtgtacca atacaacgcc tggatggcgg ccgtcgccgg
       61 cctgacggtg atcctcggag cggtgtacat gctcaacgcg taccgcaaga tctcgctcgg
      121 ggagaccggc tcgcgcacgc acagcttcga cgaccttacc ctcaacgaga aagccgtatt
      181 gatcccgatc gttgcggtca tctttattac cggtgtttat cccaaaccct tgctcgacct
      241 ggtagagcct tccgtacgcg ccttgcacga tctggtcgtg cagtcgggtg cgctctcgct
      301 gcgttgatcc ttcctacgcg acccgcatga atacgctgat catactagcc gggctgggcc
      361 tggtgctcct cctgaccgag atcttcaaca tccggaaagg ccaactcgcg ttgatcctga
      421 tcgggctggg cgcggcgctc gcagccgaca ttgccgactg gaacgcgccg gtgagcctgt
      481 tcaacgacat gatgcgcgtc gatcatttct ccgtggcgtt cacgggcgtg atcctggcgg
      541 tgtcgttgtt gtggttcacc ttctcgtctt cttacttcga cgaaccctcg agccgggtcg
      601 atcatttcgc cctgctcatc ttcgcgatca ccggcgcggt cgtgatgacc agcttttcga
      661 acatgatcat gttgttcctg ggcattgaga tcctgtccat ttcgctttac atcctcgcgg
      721 gtagcaacaa gagcaatctc gcctcaaacg aagcggccct gaagtacttc ctcatgggca
      781 gtttcgcgac cggcttcctg ctcttcggca tcgccttgtt gtacggcgcc accggttcgt
      841 tcaacatcgc ggtgatcgcc gaaaagatcg cggccaacgg actcgaccag ccggtcatga
      901 ttaccgccgg actgttcctg atgatgatcg cgatggcgtt caaagtatcg gcggctccgt
      961 ttcactggtg ggcgccggat gtttaccagg gctcgccgac cgtcatcaca gcgttcatgt
     1021 cgacggtcgt gaagacggca gccttcgcgg ccttcttacg actcttcagc ggcgcattcg
     1081 cgtcgatcgc gatcaattgg agcgagacgc tgttcatcat ctcggcgctc acgatcgcga
     1141 tcggcaacat caccgccgtt ttccaaacca gcttcaagcg catgctggcc tattccagca
     1201 tcgcgcatgc aggctatatg accatggcct tgttgtcgca gcaaggtcag tcgggcggtt
     1261 cgctcttgtt ctacacggcc gcttatagcg tgtcttcgct cgccgctttc gcgatcctct
     1321 acctggtgag caacaaaacc ggtaatgagc aactcgaggg attcaacggt ctggcttcgc
     1381 gcaatccgtt tctcgccgcc atcacggtgg tggccatgtt gtccctggcc ggaatcccgc
     1441 ccacggccgg atttttcgcc aaatactaca ttttcacggc agcgatggaa gctggtcata
     1501 cctggcttgt catcatcggc gtactcggtt cgctggtagg cgtgtattat tacttccgtc
     1561 cgctgatcgc cttgttccgc ggcacatccg acgccaccat cgagcttccg acggccacca
     1621 ggatcttcct gatcggtacg gcggtcctgg ccatcgcgct gggcctggcg ccgggctatt
     1681 tatcaggact tttgtaacag ataatcagga ctttgtaaag gtgtcggcgc aatgtcacga
     1741 ttgcgttaaa tcggtggttg tccggttttg gatataaggg gaatttccta tacttgctcc
     1801 tccaaaaatt ttaaaaaatc aaagaaatag aacgaccgca gggtcgttta ttcgtgtagt
     1861 atcctcgtca aaaagaaaca tttaaccaac aagcataaca tgagcaacaa gcaatctaaa
     1921 gctggtggaa tacaaggtct ctttgccgca ctggttattc cgctgtgcct ggtcctggga
     1981 atcgtcatct ggaatttcat catgggtaat cccagcggtt tcgaaaacag cgatccggaa
     2041 aaaggtcccc tgcccaaaga agtaatcggc acgctcaacc actacctggc cgttatgtac
     2101 aaaggtggtt tcgtcgtacc gatcctgctc ggttcgttct tcaccgttgt ggtcttcgct
     2161 atcgagcgca tgatcacgat cggcaagtcc aagggcaccg gttccgtaga gggcttcatc
     2221 atgcgcatcc gtccgctggt agcatccggc aacctcgacg gagcgaaagc ggagtgcgcc
     2281 aagcagcgcg gttcggttgc taacgttgtc agcgccggca tcgacatgta ccgcagcatg
     2341 caagccgacc gcgagttgga caaagagcgt aagatcatgg ctattcagaa agaattggaa
     2401 gaagccacca ccctggagct tccgatgctt tccaagaacc tggtcatcat ttcgaccatc
     2461 gcttccatcg cgaccctgtt gggtctgttc ggaacggtac tcgggatgat ccgggcgttc
     2521 tcggcgctgg ccaccgccgg tgctccggac gccgtagccc tgtcgaccgg tatctccgag
     2581 gcgcttatca ataccgctct
//
LOCUS       DAOTON010000387         2597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_30996_length_2597_cov_3.256098, whole genome shotgun sequence.
ACCESSION   DAOTON010000387 DAOTON010000000
VERSION     DAOTON010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30996_length_2597_cov_3.256098"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="P5292_08110"
     CDS             complement(<1..592)
                     /locus_tag="P5292_08110"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRS39129.1"
                     /translation="MSVDVLLGLQWGDEGKGKIVDVLTPEYQVIARFQGGPNAGHTLE
                     FEGIKHVLHTIPSGIFREGVMNIVGNGVVIDPVTLRREVEALQQLQVPVKDRLFISRK
                     AHLILPTHRLLDAASEALKGKDKIGSTLRGIGPTYMDKTGRNGLRVGDTEASDFQQRY
                     EKLRNKHLELLQGMQYTDYRLEELEAPFFAAITFLRD"
     gene            complement(689..1036)
                     /locus_tag="P5292_08115"
     CDS             complement(689..1036)
                     /locus_tag="P5292_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRS39130.1"
                     /translation="MDFTYKIINHGTYAIVEMSGNLIDKNQATPLLEEIQDVIEKGIN
                     NFVISMEHFRYLNSNGLNVLVNILTKSRKNGGDTTICSISDKIKELLIITKLNTVFTI
                     TEDLPAAIRQFQN"
     gene            complement(1255..1737)
                     /locus_tag="P5292_08120"
     CDS             complement(1255..1737)
                     /locus_tag="P5292_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRS39131.1"
                     /translation="MSTPDINKVRQVFTTFLEAQGHRKTAERFAILEEIYSMDEHFDV
                     ESLFVRMKNKGLSISRATLYNTIDLLLESELITRHQFGKNLARFEKSYTYKQHDHLIC
                     QDCEQIFEFCDPRIQQIQSTMGEILRFDVNHHALNLFGKCRELKETGSCSRRLSGAGK
                     "
     gene            complement(1751..>2597)
                     /locus_tag="P5292_08125"
     CDS             complement(1751..>2597)
                     /locus_tag="P5292_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5913 domain-containing protein"
                     /protein_id="HRS39132.1"
                     /translation="SGDQVEILTSNKQTPKEDWLSYVVTAKAKSKIKTALKEEKKKLA
                     EDGKEILERKFRHLKLELNHENLNELLAYYKIPTSLELFYRVAKGIIDVQDLKEFAIE
                     KGGIKARTPAKQDAPVSLEQLVKNVRGSSDNLVIGENLERIDYKLSPCCNPIPGDDVF
                     GFITVNEGIKIHRVSCPNAIRLMSNYAYRIVKAKWTNQQQIAFLAGVRLTGLDDVGVV
                     NKISKVISSELKVNMRSISIESNDGIFEGKIMLFVHDTAHLDKLIKKLKEIHGILTVG
                     RIDAA"
BASE COUNT          538 a          663 c          696 g          700 t
ORIGIN      
        1 agtcgcgcag gaaggtgatg gctgcaaaga agggcgcttc gagttcttcc agtcggtagt
       61 cggtgtactg cataccctgg agcaactcca ggtgcttgtt ccgaagtttt tcgtagcgct
      121 gttgaaagtc ggatgcttcc gtatcgccga cccgcaggcc attgcgtccg gtcttatcca
      181 tgtaggtagg gccaatgcca cgcaaggtgg atccgatctt gtcctttccc ttcagcgcct
      241 cggaagcggc atcgagtaac cgatgcgttg gaagaatgag atgcgctttg cgggaaatga
      301 acagtcggtc ctttaccgga acctgcaact gttgcaaggc ttccacttca cgccgcaggg
      361 tgaccggatc gatgaccaca ccattgccca cgatgttcat gacaccttcc cggaagatgc
      421 cgctgggaat cgtatggaga acgtgtttga tgccttcgaa ttccagcgta tgtcccgcat
      481 tggggccacc ctgaaagcgg gcgattacct ggtactcggg tgtgagaacg tcaacgattt
      541 ttcctttgcc ttcgtctccc cattggaggc cgagcagaac gtctacggac atgtcgggat
      601 gggctttcgc ctggatatta ccggtgtgga aatgaaaaca tcccgtggaa cggacacggg
      661 atgtagtttg ctatcagttt tcgctccatc agttttggaa ttgccggatg gcggccggca
      721 ggtcctcggt gatggtgaat acggtattca gtttggtgat gatcagcagt tccttgatct
      781 tgtccgaaat actgcagatc gtggtgtcgc cgccattctt gcggcttttc gtgagaatgt
      841 ttacaagtac attcagcccg ttgctgttca ggtaacggaa gtgttccatc gagatcacaa
      901 aattgttgat gcctttctca atgacgtcct gtatttcctc caacaagggg gtcgcctgat
      961 tcttatcgat caggttgccg ctcatttcta caatcgcgta ggtgccgtga ttgatgatct
     1021 tatacgtgaa atccatgcgg taaaagtaag gattctctcc ggaaaggcct taagcagttg
     1081 atttgtaatt tattaacaca tcaacgctga gaaccaacgc cgttcaatgg ttgggaagcg
     1141 acatagccgg ttacacaaca ccattaacgg acaattcaat gtaatttttt tcgtcaccag
     1201 cctaccactc accgcctacc gcctactgga aactgccatc ttctgcatta tcactcactt
     1261 cccggcaccg ctcaacctcc gactacaact ccccgtctcc ttaagctctc tgcacttacc
     1321 aaaaagattc aaggcatgat ggttcacatc gaagcgaagt atttcgccca tggtcgactg
     1381 gatctgctgg atacgcgggt cgcagaactc aaagatctgc tcacagtcct ggcagatcag
     1441 gtggtcgtgc tgcttgtacg tgtaagactt ctcaaagcgg gccaggttct tgccgaactg
     1501 atgacgggtg atcagctccg attccagcag caggtcaatg gtattataca aggtggcgcg
     1561 actgatgctg agccctttgt tcttcatgcg gacaaaaagc gattcgacat cgaagtgttc
     1621 gtccatcgag tagatctcct ccaggatagc gaagcgctcc gcggtttttc gatgcccctg
     1681 ggcttcgaga aaggtggtga atacctggcg gactttgttg atgtcgggtg tgctcacgaa
     1741 gtaggtggga ttaagccgcg tcgatacggc cgacggtgag aatgccgtgg atttctttca
     1801 atttcttgat aagcttgtcg agatgggcgg tatcgtgtac gaagagcatg atctttcctt
     1861 cgaagatgcc gtcgttcgat tcgatactga tgctgcgcat gttcaccttg agttcgctgg
     1921 agatgacttt cgagatcttg ttcacgaccc cgacatcgtc gaggccggtg agccgcacgc
     1981 ctgcgaggaa ggcgatctgt tgctggttcg tccacttcgc cttcacaatg cggtaagcat
     2041 agttcgacat cagccggatc gcgttcgggc aactcacgcg gtggatcttg atgccttcgt
     2101 tcacggtaat gaagccgaat acgtcgtcgc ccggaatcgg attgcagcaa ggcgaaagct
     2161 tgtaatcgat gcgttcgagg ttctcgccga tcacaaggtt gtcgctggaa ccccgtacgt
     2221 tcttgacgag ctgctcgagc gagaccggcg cgtcttgttt ggcgggtgta cgcgctttga
     2281 taccgccttt ttcgatggcg aattctttca ggtcctgtac atcaatgatg cctttcgcga
     2341 cccggtaaaa caattcgagg ctggtgggga tcttgtaata ggcgagcagc tcgttgagat
     2401 tctcgtggtt cagttccagc ttcaggtggc ggaactttcg ttccaggatc tctttgccgt
     2461 cttccgccag ttttttcttc tcttccttca gcgccgtctt gatcttcgac tttgctttgg
     2521 ccgtcaccac ataagagagc cagtcttctt tcggcgtttg tttgttggat gtcaggatct
     2581 ctacctggtc gccgctc
//
LOCUS       DAOTON010000388         2591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31092_length_2591_cov_2.761041, whole genome shotgun sequence.
ACCESSION   DAOTON010000388 DAOTON010000000
VERSION     DAOTON010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31092_length_2591_cov_2.761041"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1797
                     /locus_tag="P5292_08130"
     CDS             <1..1797
                     /locus_tag="P5292_08130"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39133.1"
                     /translation="LNLSSSVTPVICGQANSGAIDLTVSGGNAPYSYSWSNGATSEDL
                     SNLNSGNFTVTITDASSCTGSQTFNVGQVGTAPATPASVNGAAAVCNNQSLTYSVPAV
                     PGASTYNWTFPSGFTITGGAGSNSVTVSASNIPASGQICVTAQNACGTSAPVCFTVAK
                     VTARPATPAAIVGANIICPGSTVSFSVAADPMATSYNWQLPTGATVVSGQGTNSISFT
                     LPTPFTTGTLRCTPSNCLGAGGTRTMTVYGTPTTPGTISGPTNGVCAGSTNVNYSVSL
                     VPSASSYVWSAPPGATITSGAGTNAVTVTFSAGFTGGNLTVATVNSCGTSSSRVAYIR
                     SKPVTPGSINGTLTGICSAGAMNFSISPVTGATGYSWNVPSGISVQSGAGTTSIGVNV
                     SPAFVSGNLCVTASNLCGNSPQRCAALTAKPAVPSSISGPTAVCANQTNVAFSTASVS
                     GATTYNWVVPSGATIASGAGTNSILVNFGANGGTVSVNAGNGCGNSSNRNMAVAINCR
                     SAATATTSGNISAFPNPASELLTVQVDALEKSNSRVMLRDLTGRIVLQQDLRLIAGLN
                     QINLDVHQLARGVYVLELGQDDQRESLRVVLQ"
     gene            complement(1858..>2591)
                     /locus_tag="P5292_08135"
     CDS             complement(1858..>2591)
                     /locus_tag="P5292_08135"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39134.1"
                     /translation="ASGVGLNWQAGNGGAGVHFIPPFYPCKVTEVHAYIQANPNLVGF
                     YMTVFDDNGGSGAPGTRLDSVYADPATFTTPGWVSLQLTTPIVINSGGFYVAWNMAGE
                     FISLGQDLIGPFSNRTYEVLSNAWAIYRTRETEDLMINATVDTVNLITGMDLLDDRSV
                     SQFYPNPASGIACLQLQNTEYSAEGVSIEVFLPDGRCTLRRSGRDTGVISPLVLDVSN
                     WKSGLYFIRLTSGNQQVTRRLQVLH"
BASE COUNT          582 a          767 c          667 g          575 t
ORIGIN      
        1 ttgaatcttt catcgagcgt gacaccggtt atttgtggac aagccaactc gggggccatt
       61 gacctgacgg tcagcggtgg caatgcaccc tattcctata gctggtcaaa tggcgcgaca
      121 tcagaagatc tgtcgaacct gaacagcggc aacttcaccg taacgattac ggacgcttct
      181 tcctgtaccg gcagtcagac cttcaatgtc ggacaagtcg gtaccgcacc ggcgactccg
      241 gcatctgtaa acggagcagc ggccgtttgt aacaaccagt cgctgaccta ttccgttccg
      301 gctgtaccgg gggcatcaac ttacaactgg accttcccgt ccggattcac catcaccgga
      361 ggtgccggca gcaattccgt gacggtgagt gcttccaaca ttcccgcaag cggacagatt
      421 tgtgtcactg cacaaaatgc ctgtggaacc agcgcgccag tctgcttcac cgtagcaaag
      481 gttaccgcgc gtccggcaac tccggctgcc atcgtcggtg caaacatcat ttgccccggt
      541 tctaccgtct ccttcagtgt cgcggccgat ccgatggcaa caagctataa ctggcaactg
      601 cccaccggcg ctacggttgt cagcggtcag ggcaccaata gcatcagctt cacgctgccc
      661 acaccgttca ccaccggaac actccgttgt acgccttcga attgcctggg cgccggcgga
      721 acgcggacaa tgacggtcta tggcacaccg accactccag gcacgatcag cgggcccacc
      781 aacggagtct gtgccggtag caccaatgtg aattattcgg ttagtcttgt acccagtgcc
      841 tcctcgtacg tatggagtgc tcctcccgga gcaaccatta cctccggtgc aggaacaaat
      901 gccgtcaccg ttactttcag cgcgggattt accggcggta acctgaccgt tgctacggtc
      961 aattcctgtg gcaccagcag ttctcgcgtg gcttatatcc gcagcaaacc ggttactccc
     1021 ggttccatca acggaaccct caccggaatt tgcagcgcag gcgccatgaa cttctcgatc
     1081 tcaccggtga ccggagctac aggctattcc tggaatgtac ccagcggtat ttcggtccaa
     1141 tccggtgccg gcactacgtc gatcggtgtg aacgtctctc ccgcatttgt atccggcaac
     1201 ctgtgtgtga ccgcttcgaa tttatgtgga aacagccctc agcgttgcgc ggccctgact
     1261 gccaaacctg ctgtaccgtc cagcatcagc ggacctactg cggtatgcgc caatcagacc
     1321 aacgtcgcct tctccactgc ctccgtatcc ggagcgacta cgtataattg ggtggttcca
     1381 tccggagcta ccatcgcatc cggtgccggt acgaattcca tcctcgtcaa tttcggtgcg
     1441 aatggcggta ccgtaagcgt caatgccggt aacggttgtg gcaacagttc caaccgaaac
     1501 atggcagtgg ccatcaactg tcgttcagct gcaacagcaa ccacctccgg aaacatctcg
     1561 gccttcccga atccggcatc ggagttgttg accgtacagg tcgatgcgct tgaaaagtcg
     1621 aacagccggg ttatgctgcg ggatctgacc ggaagaatcg tactgcaaca ggatcttcgc
     1681 ctcatcgccg gcttgaacca gatcaacctg gatgtgcacc aattggcacg tggagtctat
     1741 gtgctggaat tgggacaaga cgaccaacgc gaatcactgc gggttgttct tcaataatcg
     1801 tcagctataa aaaagaaagg ccgggttcaa cgcccggcct ttcttttttc acgtaggtca
     1861 atgcaatact tgtaatcgcc ttgtcacttg ctgatttcct gatgtcagtc ggataaaata
     1921 caacccgctt ttccagtttg aaacatcgag caccagagga gagataactc ccgtatcccg
     1981 gcctgaacgg cgtagtgtgc agcgtccatc gggcaggaag acttcgatcg aaactccttc
     2041 ggccgaatac tccgtatttt gaagctgaag acaagcgatt ccggatgccg gattcggata
     2101 gaattgggaa acactcctgt catccagcaa atccatgccg gtgatcagat tgacggtatc
     2161 gacggtggca ttgatcatca gatcctccgt ttcccgtgta cggtagatcg cccaggcatt
     2221 gcttaacact tcataggtgc ggttcgaaaa aggtccgatc aggtcttgac cgaggctgat
     2281 gaattctcct gccatgttcc aggcaacata aaatccgccg ctgttgatga cgatcggtgt
     2341 tgtcagctgc aggctgaccc aacccggtgt ggtaaaggta gccggatcgg catacacgga
     2401 atccagacgc gtaccgggtg ctccgctgcc gccgttatca tcgaaaacgg tcatgtaaaa
     2461 accaaccagg ttcggattgg cctggatata ggcatgaacc tccgttacct tacagggata
     2521 gaatggcgga ataaagtgta cgcccgctcc accattgccc gcctgccagt tcaagccgac
     2581 tccgctcgcg g
//
LOCUS       DAOTON010000389         2586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31170_length_2586_cov_3.855788, whole genome shotgun sequence.
ACCESSION   DAOTON010000389 DAOTON010000000
VERSION     DAOTON010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31170_length_2586_cov_3.855788"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="P5292_08140"
     CDS             <1..193
                     /locus_tag="P5292_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39135.1"
                     /translation="TRSGIYQIEYGLGEDTLAVVIRLDPASNQPAFFLLLGDSALEML
                     DAARSPQANPIAYRLRKNR"
     gene            complement(208..990)
                     /locus_tag="P5292_08145"
     CDS             complement(208..990)
                     /locus_tag="P5292_08145"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRS39136.1"
                     /translation="MTRICCLLLVLLFQAYPLAAQRSLVASEPTTNDRFPKKVGNLRF
                     SDSVLKLGKVRTNELRSDTLKVLNTATSSIRWELPKDVPTHLRISLSGEELASGSEGW
                     IAIRYDAREKNDFGQVFDRLIFSTTDTDLPQKVITISANITEYFAPLSTEDSAVVQRS
                     RIPETVFDFGTISQGTKVEHAFPVYNDGQRELILRHTRSACGCIKTVIAKQLLAPGDS
                     TVIQLTYDSFGKEGKESRNFQLFQNDPAAPEIRFEVKGTVTK"
     gene            complement(995..2386)
                     /locus_tag="P5292_08150"
     CDS             complement(995..2386)
                     /locus_tag="P5292_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRS39137.1"
                     /translation="MSQQPTSVHFIAIGGAAMHNLALALHQLGFRVTGSDDEINEPSR
                     SRLQAAGLLPEAIGWFPDRLNYRPDAVILGMHAREDNPELQRARELGLRVYSYPEYLY
                     EHSKNKTRVVIGGSHGKTTITSMILWVLQSLDRDFDYLVGAKLEGFDTMVRITNAPLI
                     VLEGDEYLASPVDRRPKFHLYKADIALLSGIAWDHINVFPTYEQYFDQFRIYVEQVPP
                     AGKVIYCQEDPEVNRLMHIAEVGAERVPYGIPAHRIENGRTILDTTIGPVPIEVFGKH
                     NLMNLEGARLVCHALGVSDEQFYHAIASFRGAARRLEMLASNDQCAVYKDFAHSPSKL
                     KATTEAVRQQFPDRKLIALMELHTFSSLNEKFLDEYRGSMAAADEAWVYFNPHTIEHK
                     RLPSISKDRVAKAFDRGDLQVFTDSADWLDQLRNRDLRGTVLLFMSSGTFDGISLEEL
                     ARELTEKSLLPSA"
BASE COUNT          593 a          710 c          676 g          607 t
ORIGIN      
        1 gacccgatcc ggcatttatc aaatcgagta cggcttggga gaagatacac tcgcggtcgt
       61 gattcgattg gatccagcat ccaaccaacc tgccttcttc cttctgctcg gggattccgc
      121 tctggaaatg ctggacgcag ctcgaagccc tcaggctaat ccgattgcct accggctccg
      181 gaaaaatcgg taagcgaaat agtcgactta tttcgtgaca gtacccttca cctcgaacct
      241 gatttccggg gctgcaggat cgttttgaaa aagctggaag ttgcgtgact ccttaccctc
      301 tttgccgaaa gaatcgtagg tcaattgaat gaccgtacta tcacccggcg ccaggagttg
      361 tttggcgatt acggttttga tacaaccaca agcggatcgc gtatgtcgca agatcaattc
      421 ccgctgtccg tcattgtaaa caggaaatgc atgctccact ttcgtgccct gggaaatcgt
      481 accgaaatca aaaacggttt ccggaattct cgaacgctgg accaccgcag aatcttcggt
      541 acttaaggga gcaaaatact cggtgatgtt tgccgagatg gtgatcacct tctggggcag
      601 gtcggtatcc gtagtggaaa agataagccg gtcaaatacc tgcccgaaat cgttcttttc
      661 tcgtgcatcg tagcgaatgg cgatccatcc ttccgatcct gatgccagtt cttcgccaga
      721 aaggctgatc cgcaggtgag tcggaacatc tttcggcaat tcccaacgga tgctgctcgt
      781 tgcggtgttc aataccttca gggtatccga gcgcagttcg ttcgtcctca cctttcctag
      841 tttcaggaca gaatcggaga accgaaggtt tcccactttt ttcggaaacc ggtcgttggt
      901 ggtaggttcc gaagcaacca ggctgcgctg ggcggccaat ggatatgctt gaaacaagag
      961 aaccagcagg aggcaacaga tacgggtcat cggcttaggc agacggaagt aacgattttt
     1021 cagtcaattc ccgtgcaagt tcctccagag agattccgtc aaaagttccc gaactcatga
     1081 atagcaaaac ggttccacgc aggtcccggt tgcgcaactg gtccagccaa tcagcggaat
     1141 cggtgaatac ctgaagatcg ccgcggtcaa aggcttttgc cactcgatcc ttcgagatgg
     1201 atggcaaccg cttgtgctcg atcgtatggg gattgaaata gacccaggcc tcgtccgctg
     1261 ccgccatgct gccacggtac tcatccagga acttctcgtt caggctgctg aacgtatgca
     1321 gttccatcag tgcgatcagc ttccgatccg ggaactgctg acgaacagcc tcggtagtgg
     1381 ccttcaattt cgatggtgaa tgggcaaagt ccttgtaaac cgcgcattga tcattcgatg
     1441 ccagcatttc caagcgccgt gcagctcccc ggaaactggc gatcgcgtgg tagaactgtt
     1501 catccgagac acccaaggca tgacagacca gccgggcacc ttccaggttc atgaggttgt
     1561 gttttccgaa cacttcgatc ggtaccggac cgatcgtcgt atccagtatc gtgcgcccgt
     1621 tctcgatccg gtgggcgggg attccgtatg gtacccgctc cgccccgacc tcggcgatat
     1681 gcattaaccg gttcacttcc gggtcttcct ggcagtagat caccttccct gctggtggca
     1741 cttgctccac atagattcgg aactgatcaa agtactgctc ataggtcgga aacacattga
     1801 tatggtccca ggcaattccg ctcaacaagg ctatatctgc tttgtacaaa tggaacttgg
     1861 gacgacgatc taccggagaa gcgaggtatt catccccttc caacacgatc aaaggcgcgt
     1921 tggtgatccg caccatggta tcaaagcctt ccagcttggc gccgaccagg tagtcaaaat
     1981 cgcgatccaa cgattggagt acccaaagga tcatcgaggt gatggtggtc ttgccatgac
     2041 taccgccgat gacaacccgg gtcttgttct tagaatgttc atacaggtac tccgggtagg
     2101 aatatacccg aagccccaac tcgcgggctc gctgcagttc gggattgtct tcacgggcgt
     2161 gcatgccgag gatgaccgca tcgggcctgt aattcaagcg atcgggaaac cagccaatcg
     2221 cttccgggag tagtccggct gcctgtaacc gtgaacgcga cggctcgttg atctcatcgt
     2281 cggatcccgt cacccgaaat cccaactgat gcaaggccag cgcgaggttg tgcatcgcgg
     2341 ctccgccgat cgcgatgaaa tgtacagaag tgggctgttg cgacatgaac gcttctttgg
     2401 cgaaagatcg taaggatgcc gggaacgaac cgaaccggta cccgggcgat ttgcacagcg
     2461 tttcccacag tccttttgcg tattttgccg tagtctatac cacttatgaa gctccattcc
     2521 atcgataccg gtcatttcaa actcgacggc ggcgccatgt tcggggttgt tcccaaaacc
     2581 atctgg
//
LOCUS       DAOTON010000390         2586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31177_length_2586_cov_2.896089, whole genome shotgun sequence.
ACCESSION   DAOTON010000390 DAOTON010000000
VERSION     DAOTON010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31177_length_2586_cov_2.896089"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="P5292_08155"
     CDS             complement(<1..1224)
                     /locus_tag="P5292_08155"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRS39138.1"
                     /translation="MSQKAIIKEFDLGDGRKVTIETGRLAKQADGSVVVRMGDAMLLA
                     TVVSNTEPKAGADFLPLTVDYQEKFSAVGRFPGGFLKRESRMSEYEILISRLIDRALR
                     PMFPDDYHADTQVMVTLISGDKNVQPDALAGFAASAAITISDIPFNGPISEVRVARID
                     GKFVINPYTDELAKADIDLIVAGSKDSIVMVEGEMEQVSEEEMLEAIRFAHLAIVKQC
                     EVQEELRQMAGSKPKRVYSHETNDEALRERMRKELYDQVYAIARSANPNKHERSEAFS
                     KIKEAFIASIPEEQRDELKPLVDRYYFDIESDAMRNCIINEGIRLDGRKSTDIRPIWC
                     EVDYLPSPHGSSIFTRGETQSLTTVTLGSKLDEQMIDVAMYEGSTKFMLHYNFPGFST
                     GEVKPNRAPARREIGH"
     gene            complement(1303..1572)
                     /gene="rpsO"
                     /locus_tag="P5292_08160"
     CDS             complement(1303..1572)
                     /gene="rpsO"
                     /locus_tag="P5292_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRS39139.1"
                     /translation="MKLTTEAKKQIFKKFGGAETNTGTAEGQIALFTERITHMTEHMQ
                     VAKKDFSTQRALIRLVGKRRALLDYLHKNDINRYRKIIAELNIRK"
     gene            complement(1651..2496)
                     /gene="accD"
                     /locus_tag="P5292_08165"
     CDS             complement(1651..2496)
                     /gene="accD"
                     /locus_tag="P5292_08165"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRS39140.1"
                     /translation="MSWFKRIKEGITTSTKEKKETPEGLWHKCTSCKNIVPTKDHAAR
                     MYVCDQCGYHDRIGSAEYFEILFDENTSSELFPNLSSEDPLEFVDTKKYTDRIKESQR
                     KSGLQDAIRTGVGKVNGQDLVIACMDFSFIGGSMGSVVGEKIARAIDHSLEHRIPLLI
                     ISKSGGARMMEAAFSLMQMAKTSAKLTLLSDAGIPYISLLTDPTTGGVTASYAMLGDL
                     NIAEPGALIGFAGPRVVKETIGKDLPKGFQTAEFVLEHGFLDFIVDRRELKAKLAQLL
                     GFLKN"
BASE COUNT          492 a          668 c          803 g          623 t
ORIGIN      
        1 gtggccgatc tcgcggcgtg ccggagcgcg gttgggcttc acttcaccgg tcgagaaacc
       61 ggggaagttg tagtggagca tgaacttggt cgaaccttcg tacatggcca cgtcgatcat
      121 ctgctcgtcg agcttgctgc cgagggtgac ggtcgtgagc gactgcgtct cgccgcgggt
      181 gaagatcgag gaaccgtgcg gggagggtag gtaatccact tcgcaccaga tcggacgtat
      241 gtcggtgctc ttgcggccgt cgaggcggat gccttcgttg atgatgcagt tacgcatcgc
      301 gtcgctctcg atgtcgaagt agtaacggtc gacgagcggc ttcagctcgt cacgttgctc
      361 ttcggggatg gacgcgatga aggcttcctt gatcttcgag aaggcttcgc tgcgctcgtg
      421 cttgttcggg ttggccgaac gggcgatggc gtacacctgg tcgtagagtt ctttccgcat
      481 gcgctcgcgg agggcttcgt cgttggtctc gtgcgagtaa acgcgcttgg gcttgctgcc
      541 ggccatttgg cgcagctctt cctgcacttc acactgcttg acgatggcca gatgcgcgaa
      601 gcggatggct tcgagcatct cttcttcgct cacctgttcc atttcacctt ccaccatcac
      661 gatggagtcc ttcgaaccgg ctacgatcag gtcgatgtcg gccttcgcga gctcatcggt
      721 ataaggattg atcacgaact tgccgtcgat gcgcgcgacg cgcacttcgg agatcggacc
      781 gttgaacgga atgtcggaga tggtaatggc cgcggaagcc gcgaagccgg cgagggcgtc
      841 gggctggacg ttcttgtcgc ccgagatgag ggtgaccatc acctgggtgt cggcatggta
      901 gtcatccggg aacatcggac gcagagcgcg gtcgatcagt cgggagatga ggatctcata
      961 ttccgacatg cggctttcgc gcttgaggaa gccgccgggg aaacgtccga cagcggagaa
     1021 tttttcctgg tagtcgacgg tcagcggaag aaagtccgcg ccggctttgg gttccgtgtt
     1081 ggagaccacg gtggcgagca acatggcatc gcccatgcgt acaacgacgg atccgtcggc
     1141 ctgcttggcc agacgtcccg tttcgatcgt gaccttacgg ccatcgccga ggtcgaattc
     1201 tttgataatt gctttctgtg acatagtacc gctttgtgcc tcgttaaaaa cagaaaaggc
     1261 aaaccaccct ggctgccttt tccgaatctg aggatctttg aattattttc gaatgtttag
     1321 ttccgcgatg atcttccggt aacgattgat atcgttcttg tgaagatagt cgagcagggc
     1381 ccggcgctta ccgacgaggc ggatcagcgc gcgctgggtc gagaagtctt tcttcgcaac
     1441 ctgcatgtgc tcggtcatat gggtaatgcg ctcggtgaag agggcgatct gtccttctgc
     1501 cgtaccggta ttggtctcgg cgccaccgaa cttcttgaaa atctgctttt tcgcttcagt
     1561 tgtcagcttc atgttacttc ctcgaatact taagggtcaa taaacgaggt gcaaatgtac
     1621 gaaaatttcc agaatagcta gacttcccga ttaatttttc aggaagccaa ggagttgggc
     1681 cagcttggcc ttgagttccc ggcggtcgac gataaagtcc aaaaaaccgt gttccagcac
     1741 gaattcggcg gtttggaagc ctttggggag gtcttttccg atggtttctt tcacgacccg
     1801 tggaccggcg aagccgatca gagcgcccgg ttcggcgatg ttcaggtcgc ccagcatggc
     1861 gtaggaagcc gtgacaccac cggtcgtggg atcggtcaat aaagagatgt agggaatgcc
     1921 ggcatccgac agcagggtca gcttggcgga ggtcttggcc atctgcatca gggaaaaggc
     1981 ggcttccatc atgcgggcgc cgccggattt gctgatgatg agcaacggaa tccggtgctc
     2041 caggctgtgg tcgatcgcgc gggcgatctt ttcaccgacc accgagccca tcgagccgcc
     2101 gatgaagctg aagtccatac aggcgatcac gaggtcttgt ccattcactt tgccgacacc
     2161 ggtgcggatc gcgtcctgga gtccgctctt gcgctgggac tctttgatgc ggtcggtgta
     2221 cttcttcgtg tcgacaaatt cgagcggatc ttcggagctg aggttgggga agagttcgga
     2281 agaggtgttc tcgtcgaaca ggatctcaaa gtactcggcg gaaccgatcc ggtcgtggta
     2341 accgcactgg tcgcatacgt acatgcgcgc ggcgtggtcc ttagtcggga cgatgttctt
     2401 gcacgaagta cacttatgcc aaagaccttc cggcgtttcc ttcttctcct tggtggaagt
     2461 ggtgatgcct tctttgatgc gtttgaacca ggacatgtcg tttgggcttt acaacggctg
     2521 ttcggcgtgg aaaatgatca ggcgatggtg atatccttgg acaggtaaac gtcctgaatg
     2581 gcgttg
//
LOCUS       DAOTON010000391         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31225_length_2583_cov_2.590585, whole genome shotgun sequence.
ACCESSION   DAOTON010000391 DAOTON010000000
VERSION     DAOTON010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31225_length_2583_cov_2.590585"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1706
                     /locus_tag="P5292_08170"
     CDS             <1..1706
                     /locus_tag="P5292_08170"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRS39141.1"
                     /translation="VIQMSLLRGYRTGGTIHLVINNQIGFTTNYLDARSSTYCTDVAK
                     VTLSPVFNVNADDVEALVYTIELAMEFRQKFHRDVFIDILGYRKYGHNEGDEPRFTQP
                     LLYKAIAAHPNPREVYNDKLMSLGAVEADLAREMEKSFKASLQKSLDTAKQAKNKKVS
                     PYLEGSWKGLRLATPKDFDRSPDTGVDIRKLKAIGAAITRLPEGNKFFNKTERLFAER
                     AKMVESGRFDWAMAELLAYGTLLSEGHPVRFSGQDVERGTFSHRHAVVRVEDSEAQYV
                     PLANIGKEQAPFRIYNSLLSEYAVLGFEYGYALASPFSLVIWEAQFGDFMNGAQIIID
                     QYLSSAEDKWNRMNGITLLLPHGYEGQGAEHSSARLERFLNLCAENNIQVANCTTPAN
                     FFHLLRRQMHRPFRKPLIVFTPKSLLRHPRCVSDIAELSTGGFQEVMDDTTAKPNEVA
                     RVIFCSGKVYYELLEQKEKDQVNDVAIVRLEQLYPLPLKQIEAVRKKYANATDWIWLQ
                     EEPVNMGAWSYILRVLRDIPFKVIAREESASPATGSHRTHEREQRELINRAFARDLVA
                     H"
     gene            1734..>2583
                     /locus_tag="P5292_08175"
     CDS             1734..>2583
                     /locus_tag="P5292_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRS39142.1"
                     /translation="MAVVEMKVPSPGESITEVQIARWLKKDGDYVEKDEEISEIDSDK
                     ATLTLNAEASGQIRILVAEGETVPVGKVVCSIDSSVAAPAAKPKAEPKAAQAVAAVPA
                     TTPAAKPAKAAAAGIPSPAADKILREKGVQAAQVNGSGPGGRITKADAMAATPAPSQK
                     PGSREERREKMSMLRKRVAERLVAVKQETAMLTTFNEVDMSAIMAIRKQYKDTFKETH
                     GVNLGFMSFFTKAVCEALAAFPAVNARIDGSELVYNDYCDIGIAVSAPKGLMVPVIRN
                     AESLSLA"
BASE COUNT          624 a          709 c          719 g          531 t
ORIGIN      
        1 aagtcatcca gatgtcgctg ctgcgcggtt accgcaccgg cggcacgatt cacctggtga
       61 tcaacaacca gatcggattc acgaccaact acctggacgc gcgttccagt acctattgta
      121 ccgatgtggc taaggtcacc ttgtcgcccg tatttaacgt caacgctgac gatgtggagg
      181 cgctggtcta cacgattgaa ctggcgatgg aattccgaca gaagttccac cgcgatgtat
      241 tcatcgatat tcttggctac cggaagtatg gccacaacga aggcgatgaa ccccggttca
      301 cccagccctt gctctacaaa gccatcgccg cgcatccgaa cccgcgtgaa gtctataacg
      361 ataagctgat gtcgttggga gctgtggaag ctgatctggc ccgggagatg gaaaagtcgt
      421 tcaaagcatc gcttcaaaag agcctcgata cggccaagca ggcaaagaac aagaaggtat
      481 ctccctacct cgaaggctcc tggaaaggat tacgccttgc gacaccgaaa gactttgacc
      541 gttcaccgga tacgggtgtc gatatccgga aactcaaagc gatcggtgct gcgatcacac
      601 gtttgccgga aggcaataaa ttcttcaaca agaccgagcg cttgtttgcc gagcgcgcaa
      661 aaatggtcga atcaggccgc ttcgattggg ccatggctga actgttggca tacggaaccc
      721 tgttgtcaga aggtcatccg gtgaggttca gtggtcagga tgttgagcgg ggtactttct
      781 cccaccgaca tgccgtcgtg cgtgtcgaag attccgaggc ccagtatgtt ccgttggcga
      841 acattggaaa agaacaggca ccattccgta tctacaattc acttctatcg gaatacgcgg
      901 tgctgggttt tgagtacggc tacgcgcttg cgtctccttt ttcgctggta atctgggaag
      961 cgcagttcgg cgatttcatg aatggtgcgc aaatcatcat cgaccagtac ctcagcagcg
     1021 cggaggataa atggaaccgg atgaacggca tcacactgct gttgccgcac ggctatgaag
     1081 ggcagggcgc cgagcactcc agcgcccgac tcgaacgatt cctgaacctg tgcgcggaga
     1141 acaacatcca ggtggcgaat tgtacgactc ccgccaactt ctttcacttg ttgcgccggc
     1201 agatgcatcg tccgttccgc aaaccgctga tcgttttcac gccgaagagc ctgttgcgtc
     1261 atccacgttg tgtttccgat atcgccgagc tttctaccgg cggtttccag gaggtcatgg
     1321 acgacaccac ggccaagccg aacgaagtcg ctcgtgtcat cttctgcagt ggcaaagtgt
     1381 attacgagct gctcgagcaa aaagaaaagg atcaggtgaa cgatgtcgcg atcgtgcgct
     1441 tggaacagtt gtatccgctt ccgctcaagc agatcgaagc tgtgcgcaag aaatacgcta
     1501 atgcgacgga ctggatctgg ctacaggaag aaccggtcaa catgggtgcc tggtcgtata
     1561 tcctccgtgt cttacgcgat attccgttca aggtcattgc ccgggaagaa agtgcaagcc
     1621 cggctaccgg ttcgcaccga acgcacgaac gcgagcagcg cgagctgatc aaccgtgcct
     1681 tcgcacgcga cctggtcgcc cattgatttt tacccttact actccgaata atcatggctg
     1741 ttgtagaaat gaaagtgccg agccccggcg aatcgataac ggaagttcaa atcgcccgct
     1801 ggctgaaaaa ggatggcgac tatgtcgaga aggacgagga gatcagcgag attgattcag
     1861 acaaagcgac cctgacgctg aatgccgaag cctccggtca gatccggatc ctggtagcgg
     1921 aaggcgagac cgttccggtc ggaaaggtcg tttgttccat cgattcgtcc gtagcagctc
     1981 ccgccgccaa accgaaggcg gaacccaaag ctgctcaggc agttgcagcg gttcctgcaa
     2041 caactcctgc cgctaaaccg gctaaagccg ctgcagcagg catcccttct cctgcagccg
     2101 ataagattct tcgggaaaaa ggcgtgcaag ccgcccaggt gaatggcagt ggacccggag
     2161 gaaggatcac caaagctgat gcgatggccg caacacctgc cccgtcgcag aagcccggca
     2221 gcagagagga acgcagagag aaaatgtcga tgctgcgcaa gcgggtagcg gaacgtcttg
     2281 tcgcagtcaa gcaagagacc gccatgttga ccaccttcaa cgaagtcgac atgagtgcca
     2341 tcatggcgat ccggaagcaa tacaaggata cgttcaagga aactcacggc gtcaacctgg
     2401 gattcatgtc cttcttcacc aaagcggtct gtgaagccct tgccgcgttc ccggcggtga
     2461 atgcccgcat tgacggaagc gagttggttt acaacgatta ctgcgatatc ggtatagccg
     2521 tgagtgcgcc caaaggcttg atggtccccg tcatacggaa tgcggaatca ctttcgctcg
     2581 ccg
//
LOCUS       DAOTON010000392         2573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31361_length_2573_cov_3.531374, whole genome shotgun sequence.
ACCESSION   DAOTON010000392 DAOTON010000000
VERSION     DAOTON010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31361_length_2573_cov_3.531374"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="P5292_08180"
     CDS             <1..853
                     /locus_tag="P5292_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39143.1"
                     /translation="VGMTQDGNQLYVYFDNAEIFGDLGAAPLKGKAWQRYNLLPPQVN
                     SKQEESAAALSADGNLLIFSSDRKEGQGGRDLWMIRREKGEWSEAINLGATINSKFDD
                     DAPFLTLDGQTLYFASKGHNSMGGYDLFRSSFDAKTGTWSAPLNLGYPVNTPDDNRFI
                     SFTADGKSAYVSCSRKGGLGDRDIWQLTFDDKASFPVRTLISGNITSSTGAKVELTKA
                     VLEDANGQPVQEYKPQVATTHFYLAVDPGTYKLRLEGYNFENHAEDVQVTDAPLELNI
                     TVKAAAK"
     gene            850..1626
                     /locus_tag="P5292_08185"
     CDS             850..1626
                     /locus_tag="P5292_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRS39144.1"
                     /translation="MSRKLQLKRPLVFIDLETTGTNIGTDRIVEIALLKVQPNGNRES
                     KTMRINPTVPIPVESSRIHGIYDADIQDCPTFQDVARDLQALIGNADLAGYNSNKFDI
                     PLLIEEFTRSGIHFEIADRKLIDVQNIFHKMEQRTLSAAYKFYCGKDLLNAHSAEADT
                     FATCEVLEAQLERYDSLQTDVDWLAQFSSMNKNVDLAGRIVYNDKGDEVFNFGKYKGR
                     LVSEIFRNEPSYYDWMMKGDFATNTKNVITQIRLKDFNNK"
     gene            1632..2243
                     /locus_tag="P5292_08190"
     CDS             1632..2243
                     /locus_tag="P5292_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRS39145.1"
                     /translation="MKILCIGRNYAEHAAELKNDIPAEPVFFLKPDTALLPPGQAFYL
                     PDFSNDIHHEVELVLRIGKEGKHIQEEFASRYIDGITVGVDFTARDVQQRQKEKGLPW
                     EPAKAFDHSAPVGRIVPFESFLGNDAIHFSLTRNGQEVQRGDSSLMLFPFNRILAHLS
                     RYITLRKGDLIFTGTPKGVGAVAAGDTLEAFLEGESLLRFDVK"
     gene            complement(2280..>2573)
                     /locus_tag="P5292_08195"
     CDS             complement(2280..>2573)
                     /locus_tag="P5292_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HRS39146.1"
                     /translation="SGFAKMVPDSMKANETKFEITGEKIEGDKATVTYKEEGREGETP
                     LSLVRIDGKWKVSMSKESMSGADAGGSMDIGATSMDSSAGPEPEPEAVDSVSN"
BASE COUNT          638 a          713 c          638 g          584 t
ORIGIN      
        1 cgtcgggatg actcaggacg gaaatcaact ttatgtctat tttgacaatg cggagatctt
       61 tggcgatctc ggtgccgcgc cgttgaaagg aaaagcctgg caacggtaca acctgcttcc
      121 gcctcaggtc aactccaagc aagaagaaag cgctgccgcc ctttcagcag acggcaacct
      181 gctgatcttt tccagtgacc gaaaggaagg tcaaggcggt cgtgatctat ggatgatccg
      241 acgggagaaa ggtgaatggt cagaagccat taatctggga gcaaccatca attcaaagtt
      301 cgacgatgat gcaccgtttt tgaccctgga tggtcaaacg ctctattttg ccagcaaggg
      361 acacaacagt atgggaggtt acgacttgtt ccgcagttct tttgacgcga agaccggtac
      421 ctggtccgct ccgctgaacc tcggctatcc ggtcaacact ccggatgata accgtttcat
      481 ctcgttcacc gctgacggta aatccgcgta tgtaagttgt agccgaaaag gcggattggg
      541 tgatcgcgac atctggcagt tgaccttcga tgacaaagct tcatttccgg ttcggaccct
      601 gatcagtggc aacatcactt cttcgacagg agcgaaagtg gaattgacca aagccgtgtt
      661 ggaggacgcg aatggacaac cggtccagga atataaaccc caggtggcta ccacgcactt
      721 ctacctcgcg gtagatcccg gcacctacaa actacgtctc gaaggctaca acttcgagaa
      781 tcacgcggag gatgtccagg taaccgacgc gccgctggaa ttgaacatca ccgtcaaagc
      841 tgcagccaaa tgagtcgtaa gcttcaatta aaacggccgt tggtcttcat cgatctggaa
      901 acgaccggaa ccaacatcgg cacggatcgc atcgttgaaa tcgctttgct gaaagtccag
      961 ccgaacggta atcgtgaaag caagaccatg cggatcaatc cgactgtccc gatacctgtt
     1021 gaatccagcc gtatccacgg gatctacgat gccgacatcc aggactgccc aaccttccag
     1081 gatgtagcac gcgacctgca ggccttaatc ggtaatgctg atctggccgg atacaattcc
     1141 aacaaattcg atatcccgtt gttgatcgag gaattcaccc gttccggaat ccattttgag
     1201 atcgctgacc gaaaattaat cgacgtgcaa aacatctttc acaagatgga gcaacgcacc
     1261 ttgtcggcag cttataaatt ctattgcggg aaagacctgt tgaatgccca ttccgccgaa
     1321 gccgacacct ttgccacctg tgaggtgctc gaagcccagt tggagcgata cgacagcctg
     1381 caaaccgacg tcgattggct ggcccagttc agcagcatga acaagaacgt ggaccttgcc
     1441 ggccgtatcg tatacaacga caaaggtgat gaagtattca acttcggtaa gtacaaaggg
     1501 cgcctggttt ccgaaatttt ccgaaacgaa ccgtcctatt acgactggat gatgaaaggt
     1561 gatttcgcca ccaataccaa gaatgtcatt acccagatcc ggttaaagga tttcaacaac
     1621 aagtaacccg gatgaagatt ctgtgcatcg gccggaacta cgccgaacac gcggcggaat
     1681 tgaagaacga tatccccgcg gaacccgtat tcttcttaaa gcccgatacc gccctcctgc
     1741 ctccgggaca ggctttctac ctgcctgatt tctcaaacga catccaccac gaggtggagc
     1801 tggttcttcg catcggcaag gaaggaaagc acatccagga agaatttgcc tcccggtaca
     1861 tcgatgggat cacggtagga gtcgatttca cagctcggga tgtacagcag cgccagaagg
     1921 aaaagggcct gccctgggag cccgccaaag cctttgatca ttccgctccg gtcggacgaa
     1981 tcgttccgtt cgagtcgttc ttaggaaatg acgccattca tttttccctg acccgaaacg
     2041 gccaggaggt gcaacgtggc gacagttcgc tcatgctctt tccttttaac cgcatccttg
     2101 cacacctctc ccgctacatt acactccgta aaggtgattt gatcttcacc gggaccccga
     2161 aaggggtcgg ggcggtcgcg gcaggtgata cgctcgaagc cttcctggaa ggcgagtcgt
     2221 tgttgcgttt cgatgtaaaa taaaaaagcc ggctgtccgc cggctttatc tgtgttcatt
     2281 caattggaaa cactgtccac cgcctccggt tcaggttccg gtccggcaga actatccata
     2341 ctggtagcac cgatatccat ggagccgccc gcatccgcac cgctcatgct ctctttactc
     2401 atggacacct tccacttacc gtcgatgcgt accagactga ggggcgtttc accttcccgg
     2461 ccttcttcct tgtaagtcac ggtcgccttg tcaccttcga tcttttctcc ggtgatttca
     2521 aacttggtct cattcgcctt catggaatcc ggaaccattt tggcaaaacc gct
//
LOCUS       DAOTON010000393         2573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31365_length_2573_cov_2.996426, whole genome shotgun sequence.
ACCESSION   DAOTON010000393 DAOTON010000000
VERSION     DAOTON010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31365_length_2573_cov_2.996426"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="P5292_08200"
     CDS             <1..845
                     /locus_tag="P5292_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRS39147.1"
                     /translation="KSLRPLSGYVLLLISLLALAGSVYAFILQLPVVGGVLLLTSVFL
                     MCGFMVVNPNESRVLTLFGDYKGTVKANGFFWVNPFMTSKKVSLRARNLNGQALKVND
                     KIGNPIEIAAVIVWQVEDTCKAVFDVDDYQTYVSIQSEAAVRHLAGTCPYDNFEDNDA
                     VVTLRGSAEKVHDMLVTELNERLSLAGIRVSDARISHLAYAPEIAGAMLQRQQATAVV
                     AARQQIVEGAVGMVQMALERLSQHDIVELDEERKAAMVSNLLVVLCGDKNVSPVVNAG
                     TLHN"
     gene            876..1070
                     /locus_tag="P5292_08205"
     CDS             876..1070
                     /locus_tag="P5292_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HRS39148.1"
                     /translation="MANEKKAFILRVSPDLLKALEKWAGDEFRSVNGQVEYLLHKALA
                     ESGRLRKKQDGKSGKKDGDH"
     gene            complement(1074..2096)
                     /gene="ruvB"
                     /locus_tag="P5292_08210"
     CDS             complement(1074..2096)
                     /gene="ruvB"
                     /locus_tag="P5292_08210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRS39149.1"
                     /translation="MSNPNLDPNKDQLSTTEQEIEKALRPQGFEEFTGQAAILDNLRV
                     FVEAARLRDEALDHVLLHGPPGLGKTTLAYILANELGVNIRTTSGPVLDKPGDLAGLL
                     TNLEPNDVLFIDEIHRLSPVVEEYLYSAMEDYRIDIMIETGPNARAVQIKLNPFTLIG
                     ATTRSGLLTAPLRARFGITSRLEYYPASLLQRILQRSADILRAPMQEDAAHEIARRSR
                     GTPRIANALLRRVRDFAQIKGNGTITLEIAHLALSALNVDQHGLDEMDNRILAAIVEK
                     FKGGPVGLSTIATAVGEEAGTIEEVYEPFLIQEGYLKRTPRGREATEKAYLHLGASQK
                     RPGELF"
     gene            complement(2138..>2573)
                     /locus_tag="P5292_08215"
     CDS             complement(2138..>2573)
                     /locus_tag="P5292_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39150.1"
                     /translation="EGDISYITNGQLKTTFLTFSGEYIFYSKYPWQFSAIPFQAGIGG
                     AHYEYNSRLDTNRRVSTPDEFLFILQPELTGQFNIVRWFGLTANAGYRFTAYSSRELR
                     EDFSAPAFSAGVKIFIDEIYKSLFPNGIFRKKRPTPVEEWER"
BASE COUNT          550 a          710 c          754 g          559 t
ORIGIN      
        1 aaaaatccct ccgccccctt tccggttacg tcttgttact catcagcctg ctggcgctgg
       61 ccggatccgt ctatgctttc atactccagt tgccggtggt cggcggcgta ctgctgctga
      121 catcggtttt cctgatgtgc ggcttcatgg tggtcaatcc gaacgaatcg cgggtgctga
      181 ccctcttcgg cgattacaaa ggcaccgtca aagcgaatgg tttcttctgg gtgaacccat
      241 tcatgaccag caagaaggta tcacttcgcg cccgcaacct caacggacaa gccctcaagg
      301 tgaacgataa gatcggtaac ccgatcgaaa tcgcagccgt catcgtctgg caagtcgagg
      361 acacctgcaa agcggtcttc gatgtcgacg attaccagac gtatgtctcc atccagagcg
      421 aggccgcggt acgtcacctc gccggcactt gtccctacga caacttcgag gacaatgatg
      481 ccgtcgtgac cctccgcgga tcagccgaga aagtacacga catgctggtg accgaactga
      541 atgagcgact ttcactcgcc ggtatccggg tatcggatgc ccgcatcagc cacctggcgt
      601 acgcacccga gattgccggt gcgatgctgc aacgacagca agccaccgcc gtggtcgccg
      661 cccggcagca gatcgtggaa ggcgccgtgg gcatggtaca gatggccctg gaacgtttgt
      721 cgcagcacga tatcgtcgaa ctcgacgaag aacgaaaagc ggcgatggtc tccaacctgt
      781 tggttgtctt gtgcggcgat aagaatgtga gcccggtggt caacgcgggg acgctccaca
      841 attgatgtta tcggctttgg tctaatcact cctgaatggc taacgagaaa aaagcgttta
      901 tcctgcgggt cagtccggac ttgctcaaag ctcttgagaa atgggccggt gatgaattcc
      961 gcagcgtgaa cggccaggtc gaatacctgc tgcacaaagc gctggctgag agtggacggc
     1021 tgcgaaaaaa acaggatggt aagagcggaa aaaaagatgg cgaccactaa aagttagaac
     1081 aactctcccg gacgtttttg tgaggcaccg agatgcagat aagccttctc ggtggcttcc
     1141 cgtccgcgcg gggttcgttt gaggtaacct tcctgaatca aaaaaggttc gtacacttcc
     1201 tcgatggtac cggcttcttc gccgacggcc gtagcaatgg tactgaggcc gacaggaccg
     1261 cctttgaact tttctacgat cgcggcaagt atccggttgt ccatctcgtc cagcccgtgt
     1321 tgatccacgt tcagcgcgga caacgccagg tgagcaatct cgagcgtaat ggttccgttt
     1381 cctttgatct gcgcgaagtc gcggacgcga cgcagcaacg cgttggcgat acgcggggtg
     1441 ccgcgactcc gtcgggcgat ctcgtgcgcg gcgtcttcct gcatcggcgc gcgcaagata
     1501 tcggcactgc gttgcaagat gcgttgaagc aaagaggccg gataatattc cagtcgggat
     1561 gttattccga agcgggcacg cagcggcgcg gtcaatagtc ccgaacgcgt cgtagcgccg
     1621 atcagggtga agggattgag tttgatctgc accgcccggg cattgggacc ggtctcgatc
     1681 atgatgtcga tccggtagtc ctccatggcg ctgtagagat actcctccac taccggactt
     1741 agacggtgga tctcgtcgat gaacaggaca tcgttcggct cgaggttggt cagcaatccg
     1801 gccaggtcgc cgggcttgtc caataccggg cccgaggtgg tacggatatt tacacccagt
     1861 tcgtttgcca gaatataggc aagggtcgtc ttcccgagac cgggaggacc gtgtaagagc
     1921 acatgatcca aggcttcgtc tcgcaatcgc gccgcctcaa cgaagactct gaggttgtcc
     1981 aggatcgcgg cctgcccggt aaattcctcg aagccctgtg gccgcaatgc tttctcgatc
     2041 tcctgctcgg tagtcgagag ctggtctttg ttcgggtcga ggttcgggtt ggacatgacg
     2101 ttccaaaggt acggactctg atggctggca gctcagctca tcgttcccat tcttccacag
     2161 gcgtggggcg cttttttctg aaaatcccgt tgggaaacag gctcttgtag atctcgtcga
     2221 tgaagatctt gactccggcg gaaaaggccg gggcgctgaa gtcctcccga agttccctgg
     2281 atgaataggc cgtgaaccgg taaccggcgt tggcggtcaa tccgaaccat cgtacgatgt
     2341 tgaactgccc ggtgagttcc ggctggagga tgaaaagaaa ttcatccggc gtggaaaccc
     2401 gccggttcgt gtcgagccgg ctattatact cgtaatgcgc cccgccgata ccggcctgga
     2461 acggaatcgc ggagaattgc cagggatact tcgaatagaa gatatactct ccactgaaag
     2521 tcaggaacgt ggtcttcagt tgaccgttgg tgatgtagga gatgtccccc tcc
//
LOCUS       DAOTON010000394         2569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31424_length_2569_cov_2.536993, whole genome shotgun sequence.
ACCESSION   DAOTON010000394 DAOTON010000000
VERSION     DAOTON010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31424_length_2569_cov_2.536993"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..136)
                     /gene="pgk"
                     /locus_tag="P5292_08220"
                     /pseudo
     CDS             complement(1..136)
                     /gene="pgk"
                     /locus_tag="P5292_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010313479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
     gene            complement(1193..1930)
                     /locus_tag="P5292_08225"
                     /pseudo
     CDS             complement(1193..1930)
                     /locus_tag="P5292_08225"
                     /inference="COORDINATES: protein motif:HMM:NF023260.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2355..>2569)
                     /locus_tag="P5292_08230"
     CDS             complement(2355..>2569)
                     /locus_tag="P5292_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRS39151.1"
                     /translation="AASILAKTYRDDLMHRLHGEFPMYGWDRNKGYGTREHRNAIRIF
                     GNSPFHRKSFRLLPEQGELPFQQEGV"
BASE COUNT          549 a          695 c          721 g          604 t
ORIGIN      
        1 ccttaccttc gagcaattcc aggctggccc cgcctccggt tgagatgtgc gacatgcgct
       61 cggccaggcc cgccctggcc accgccgccg ccgaatcgcc gccgccgatg atcgtcgtgg
      121 ctttcccgcg caggtcgaat tgcttgaatt tcccgtcatc cgacagcgcg atcagcgtgt
      181 tggcagcaga ggtggtagca gcctgcaggg gcagggagaa gttccctgcc ggacgatccg
      241 agttccagtc cggatccggt atgccgttca ggcgataatg gtaaactgtg ccgttgctgc
      301 agggtatcag gatacccgga tctgtatgtc ccggccgatt tatcagactc aagctgccgg
      361 tggctgaggc cgggagccgg atggggtagt tcgctacacg cttgccctcg cggtccagga
      421 tgaatagtcg agatttcgta ttgaataata actgccagct cccctgccga tagtagtcga
      481 ccgtatgcag tgttccaagt aaggtatcat tcaccagtac tttccacagc agcttacccg
      541 attcattaaa aaggtaaagt cttcccgact tgtcttgcgc tccaacgtag catctgccgt
      601 tttcatcgcc gggtagcagg aaaggaccgg catgcagcgt agtatccaat tgggtactcc
      661 agaccacggc cggttcccgt tccggtgttc cgccggtggt caacgcggcc tgcagttgaa
      721 gtagccggtc gctgcgcagc cgtgccgtga tgaatacatc ctgtaaatgt gttgcacgtg
      781 ttggtttgct atacttgttg aggaaagcgg gcaacaggtt ctgactccgg ttggcggagg
      841 cgtaaacgaa caggtgttga tcatttccga gacggcggag gtgttgggta aagcctgtct
      901 tggcatccaa gcgcttaccg tctgccagat catccagtag tgtcttaagg ctggctgcct
      961 gcgccgcgca aacgaaatag ccattcacga aagtgagggc gctttgcgtc caggaagcgc
     1021 tgacattgcc cagcgtcagc aacgcccagc ggtggtccgg aaaagtgaga atggtttttc
     1081 cccggtagct ttcttttcgg ataccgctgt tccggatctt gctccattgc tccagcatgc
     1141 gctcggtttc agatggtcgc atactgcgca acaacacgag taggttgttg gtaaagtcgc
     1201 taccagccgg ttccgtgatg gcaagcccgg cttccgagcc ggtcattccg tcgaactcgg
     1261 ccgcgaggtc tcgttgacag gcttggatca gggcatcgcg ttccgattcc atgagccggg
     1321 cggccggata gatgttcagg tcttgcctga agcggctccg gatcgtttgc aactgatcgc
     1381 tgccgatcca cagcagaaag gccgtacggt ccggcagtgc ggccgccatg ttgaatcgtt
     1441 gtggctgact gttctgaaaa gcggtgatcc agtcggacgg atcgacgctg ccacaaactc
     1501 cttcgagccg gatctcttct ttcagaatcc tgagttgcag gccgctccaa cgaccaagac
     1561 gactggcaag ttccaagact tcattctgct ccgtgttcca gttgctgctg agcaagtcgc
     1621 gtgctccgat gctgttaatg taaagtcgga tggcggactt attggttgca ccggcaacct
     1681 gattgaatat ccgcaccttt cgcaaaggtg taccgtttcg cgattgccgg atggcatctt
     1741 cgagtagtac cggactttca ctgagtagaa agacaccttt ggaaatggcg aagcaaaccg
     1801 gtgaagtggt atggccactc tcgtatatga tcacgtcctc gtactcacgc tcctcgaatt
     1861 cgcgatctgc aaagtactcg ctgacttgtc cggctacctc tttataggaa aatccaagcg
     1921 gtatgttcat tgcagcgagc cagcccgctc cagcgctgcc ggtcagatgc agggagagat
     1981 acagcggttg tgttgaccag gcctttttat aagcagtatg tgttccgctg accgaatccg
     2041 agaaatgcag aaccgaccgc atttggagta gatcaggatc gccaccccag ttttcccaaa
     2101 agtgagtcga ttgaagttcc tcatttgccg ctttacccgt ctgacattcg atgatcagcg
     2161 cagcattggt cggaatagca ttgaaaggat cggacagaat gacgcgatag cgttggtaat
     2221 aataaaaccc gatagccgcg gaaataatga caagcagtgc tatgaccgct ccagtgacaa
     2281 tccttctcat atactgatcc ccggttcaaa gatgatgaac ccatcgcaat gaagggggaa
     2341 aagcccaagt ggttttagac accttcctgt tgaaatggaa gttcaccctg ttccggcagt
     2401 aggcggaagc tctttcgatg aaacggtgag ttaccaaaga tccggatcgc attacggtgc
     2461 tcccgggttc cgtatccttt gttgcggtcc cagccataca tcggaaactc gccatgcagc
     2521 cggtgcatga ggtcgtcccg ataggttttc gcgagaatcg aagccgctg
//
LOCUS       DAOTON010000395         2564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31514_length_2564_cov_3.360303, whole genome shotgun sequence.
ACCESSION   DAOTON010000395 DAOTON010000000
VERSION     DAOTON010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31514_length_2564_cov_3.360303"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="P5292_08235"
     CDS             <1..823
                     /locus_tag="P5292_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39152.1"
                     /translation="ISSFPPNGVAQTEICALAVAPNDPDVLVAARRVRYEFGVNGDVF
                     RTTNQGGSWTNITAGLPDSLYYTSVDIHPANAGDIAISLAGFAAGQKVYRSTDGGQSW
                     QNVSFNLPNLPVNCVKFVPRSGQLMIASDVGVWTLDSGSVTWNDQSLGLPNVIVSDIE
                     FNEVLNKVYVCTFGRGIWGSDLGLVTGSSGLPATAIDFGVYPSLNNGRFTLRLTESLL
                     QPAAKVTITDIQGRQVWQEELNETIHEFNLGLAPGKYYIQVRTGDRVGVRSFIVG"
     gene            complement(885..>2564)
                     /locus_tag="P5292_08240"
     CDS             complement(885..>2564)
                     /locus_tag="P5292_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39153.1"
                     /translation="TQLCAPAGYSSYLWSNGATTQCITVNCSGNYSVTVSNGSCTSSC
                     SVCVTVRPRPSVCITGCTRICQGQTTSLCASSGYSSYQWSNGATTRCINVSAPGTYSV
                     VVTNGSGCTASASTTVTVGSAPSCNISGNTTICQGSSTTLCGPSGNYSYYWSNGSRNR
                     CITVNCAGTYTLTVSNGSCSSTCSVTVTVSSAPSATITGNLRICGGQTTSLCAPSGTG
                     YTYRWNTGSTSRCITVGTPGNYTVTVTNAAGCTRSSTVCVTSSPGISCNITGNTTICQ
                     GSSTSLCGPSSCASYRWSTGATTRCINVSTAGTYTLTVTNSGGCSSTCTATVTVAPRP
                     SSTITGPTVIRQGCPISLCAPSGGASYRWNTGATTRCITVNCAGTYSVTVTNAAGCSS
                     TSSICVTWYQRHHCRTVVDPKTGGVTAEVTGGIPPYTFAWSNDATESSDHTTVKHSGT
                     VEVTVTDAEGSVVKATADVLLDLLSVSAYPNPVSERATIEFTNEGLSDKAVVSIYTLD
                     GSLVTELYNDEAMSKHLYRVTWDAADIVPGVYVYRIVCGSKSFEGKLTVQH"
BASE COUNT          522 a          727 c          774 g          541 t
ORIGIN      
        1 gatcagcagc tttccgccga acggtgtggc gcaaacggag atctgtgccc tggccgttgc
       61 gccgaacgat ccggatgtac tggtcgcggc ccggcgtgta cgctacgagt ttggcgtcaa
      121 cggcgatgtg ttccgcacca ccaatcaggg cggcagttgg accaacatta ccgccggctt
      181 acccgatagc ttgtattaca cctcggtcga catccatcct gcgaatgccg gcgacatcgc
      241 gatctcgctc gcgggtttcg cggccggaca aaaagtttac cgcagtaccg acggcggtca
      301 gagctggcag aacgtttcgt tcaacctgcc caacctgccg gtgaactgtg tgaagttcgt
      361 tccgcgcagc ggccagctca tgatcgcctc cgacgtaggt gtctggaccc tcgacagcgg
      421 atcggttacc tggaacgacc agagcctcgg ccttcccaac gtgatcgtat ccgacatcga
      481 attcaacgaa gtcctgaaca aggtgtacgt ctgcaccttc ggccgcggta tctgggggag
      541 cgatctggga ttggtgacag gttcttccgg ccttcctgcc actgcaatcg attttggcgt
      601 ttacccttca cttaacaacg gccgctttac actccggttg accgaatcgc tcctgcaacc
      661 tgctgccaag gtgaccatca ccgacatcca gggccgacaa gtatggcagg aagagttgaa
      721 cgaaacgatc catgaattca acctcggtct tgctccgggc aagtattata tccaagtgcg
      781 tacgggcgac cgggtcggcg ttcgatcgtt catcgtaggt tgaatgccat aaaaaaagag
      841 cggccgcaat agtgcggccg ctcttttcat ttaccataac tcaatcaatg ctggacggtg
      901 agcttgccct caaaggactt gcttccgcaa acgatccgat agacgtaaac acccggaaca
      961 atgtccgcag catcccaggt gacgcgatag agatgcttgc tcatcgcttc gtcgttgtac
     1021 aactcggtga ccaggctgcc atccagtgtg tagatggata caacggcctt gtcgctgaga
     1081 ccttcgttgg tgaactcgat cgtcgcccgt tctgaaaccg ggttcggata ggcgctaacc
     1141 gacaggaggt caagcaggac gtcggcggtt gccttgacaa ccgagccttc agcatccgtg
     1201 acggtcactt cgaccgttcc ggaatgtttc accgtggtat gatcagagct ttccgttgcg
     1261 tcgttgctcc aggcgaacgt gtacggcggg ataccgccgg taacctcagc cgttacgcca
     1321 ccggtcttcg gatcgacgac ggtacggcag tggtgacgct ggtaccaggt tacgcaaatg
     1381 ctgctggtgc tgctgcatcc cgcggcgttc gttacggtta ccgagtaggt gcctgcgcaa
     1441 ttcaccgtga tgcagcgcgt ggtggcaccg gtattccagc ggtagcttgc accgcccgaa
     1501 ggcgcgcaga gggaaatcgg acaaccctga cgaatgaccg tcgggccggt gatcgtactc
     1561 gaaggacgcg gagcaaccgt taccgttgcc gtgcaggtac tgctgcaacc gccgctgttc
     1621 gtgactgtca gcgtgtaggt gcctgcggtg gaaacgttga tgcaacgggt ggtcgcgccg
     1681 gtgctccagc ggtagctggc gcagctcgaa ggtccgcaga gactcgtgct tgaaccctgg
     1741 cagatcgtgg tgttaccggt gatgttacac gagatgcccg gactgctggt gacgcaaacc
     1801 gtactcgacc gggtacaacc ggccgcgttg gttaccgtga ccgtatagtt gcccggcgta
     1861 ccgaccgtga tgcagcgtga tgtggatcct gtgttccagc ggtaggtgta acccgtgccc
     1921 gaaggagcgc agaggctggt cgtttgaccg ccgcagatac gcaggttgcc ggtgatcgtt
     1981 gccgacggag cggacgatac ggttaccgtg acactgcaag tgctgctgca actgccgttc
     2041 gaaacggtga gcgtataggt gccggcgcaa ttcaccgtga tgcagcggtt gcgggaaccg
     2101 ttgctccagt agtagctgta attgccggaa ggaccacaca gcgtcgtgct gctaccctgg
     2161 caaatggtcg tgttgccgct gatattgcag ctcggggcgg agccgacggt taccgtcgtg
     2221 ctggcgctgg cggtacaacc gctgccgttc gtcacgacta ccgaataggt tccgggagcg
     2281 ctgacattga tgcaacgtgt ggtagcgcca ttgctccact gataggaaga ataaccgctc
     2341 gaagcgcaaa ggctggtggt ttgaccctgg caaatacgcg tgcaaccggt aatgcaaacc
     2401 gaaggacgcg gacgcaccgt cacacatact gaacaactgc tggtacagct tccattggaa
     2461 accgtcaccg agtagttgcc gctgcaattg acggtgatac actgtgtggt cgcgccattg
     2521 ctccagagat aggagctgta tccggcagga gcgcacaatt gtgt
//
LOCUS       DAOTON010000396         2563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31538_length_2563_cov_3.110048, whole genome shotgun sequence.
ACCESSION   DAOTON010000396 DAOTON010000000
VERSION     DAOTON010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31538_length_2563_cov_3.110048"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..806
                     /locus_tag="P5292_08245"
     CDS             162..806
                     /locus_tag="P5292_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS39154.1"
                     /translation="MDRIIRVFLVDDHQVLLEGIRSLLMQAPGIEVAGIAVSANAALE
                     AFQSSQPDVLLTDVQLPGMDGAALTREVKRLYPSVMVCALSMSGDRETIGKLLDAGAT
                     GYILKNTGQEELLQAIRTVARGELYFSDEVSAEMMRALASRKEQPVAPQPVLTSREIE
                     IVRLIAKEFSNARIAEALFISERTVETHRKNIFRKTGTKSVVGLLKFAMEQNLL"
     gene            932..1294
                     /locus_tag="P5292_08250"
     CDS             932..1294
                     /locus_tag="P5292_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39155.1"
                     /translation="MNRIFLMVLCCTLFFAGLYAQPIADPKLIEQRVHAELDAWHQDP
                     AQLSFVARHFPEVRGTVVADITLSGKGRIETFFLSETDIKEPRFLEYLQKQVKKHKLK
                     TVLHKGARQKVRHRFVWN"
     gene            1319..>2563
                     /locus_tag="P5292_08255"
     CDS             1319..>2563
                     /locus_tag="P5292_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRS39156.1"
                     /translation="MKKQLLALVTVALLGTGYVRAQDDEFPQVWEAEFTNKAKVLSVV
                     NSDGSMIVGSDENEVSVLDGQGKQLWYSRFKDLTDKEGVKNGELQNIYFDAGMLFLFE
                     KKMGKDKLTVVDIKAGKALWTTDRYSDLTEDNIEYIPQLGAFFFSLKNTTVLVDAKTG
                     QQRWETDKFKGSVGAFLYDDSRNELLMINYKPSQLAALFSGFKNQLIRINAANGEVKW
                     STSFFGFVERKVLTREALASLTLKGGKVFLELDGIQVFDHETGQMLWSAVYESDEEAR
                     RGLFNRGPHGGRVIKGGIYGAIAEPLYTNDGVYVVLGSGKLRTKFIAKYDLESGKKLW
                     ESEKITGAGAMPNLHLEDGRLVAQIGGIVNKQTIENKVETGPNGTVTTIYYNNEWDFM
                     GGYGIIALDPSSGAKVWRSEKFD"
BASE COUNT          644 a          613 c          734 g          572 t
ORIGIN      
        1 ggaagacgac ggccggggta tgccaggaga catggccggg aatgctgaag gcattggaat
       61 gaaaaatgtc cgcacccgtg tgcactatct tggtggcgag ggttattggg atagccagac
      121 agggaagggg acaaccgtca atattgaaat tccactgggt aatggatagg atcatacgag
      181 tgtttctggt ggatgatcac caggtcctgc tggagggtat ccgttctttg ctgatgcagg
      241 cgcccggcat tgaagtggca ggcattgctg tttccgctaa tgctgccctg gaagcgttcc
      301 aatcctcgca acccgatgtg ttgctgacag acgttcaatt acccggaatg gacggcgcgg
      361 ccctgaccag ggaggtgaag cgcttgtacc catcggtcat ggtttgcgcc ctcagcatgt
      421 caggcgaccg tgagacgatt ggaaaactgc tggatgccgg agcaaccgga tatatcctca
      481 agaataccgg acaggaagaa ctgcttcagg cgatccggac ggtagcccgg ggtgagttgt
      541 atttttccga cgaggtgtct gccgaaatga tgcgcgccct cgcctcgcgt aaagagcagc
      601 ccgtcgcacc tcaaccagta ctgacctcac gggaaattga gattgtccgc ctgatcgcaa
      661 aggaattttc aaacgcccgg atcgcggaag cactgttcat ctctgaacgg acggtggaaa
      721 cccatcggaa gaacattttc cgaaagaccg gaaccaagtc ggtcgtcggc ttgctcaagt
      781 tcgcgatgga gcagaattta ctttgatatt ttactgattt caacattatc cggccgctat
      841 ccgtgctggt gggtatgcga tttacccgga ggacgggatt gataggacct gttcgcagat
      901 acatttttgc aacgggaaat atcagttgtc tatgaatcgt atcttcttga tggtgctttg
      961 ttgcaccctg ttttttgcgg ggctttatgc tcagccgata gcagacccta aattgatcga
     1021 gcagcgtgtg cacgcagagt tggacgcctg gcatcaggac ccggctcaat tgtcgttcgt
     1081 agcccgacat tttccggagg tgcgtggaac ggtggtcgcc gacatcaccc tttccggaaa
     1141 gggacgcatc gagacctttt ttctaagcga gacggatatc aaggaacccc gcttcctgga
     1201 gtacctgcag aagcaggtga agaaacacaa attaaagacg gtattgcaca agggcgctcg
     1261 acagaaagtg cgtcaccgct ttgtctggaa ttaaccccta acacaaatac gtacgaaaat
     1321 gaaaaagcaa ttactcgcgc tggtcacagt cgcgctgctc ggaacgggat atgtccgcgc
     1381 tcaagacgat gaattcccgc aagtatggga agcagaattc accaataagg ccaaagtgct
     1441 ctcggtcgtg aattccgacg gatccatgat cgtcggatcc gacgaaaatg aagtcagtgt
     1501 actcgatggt caagggaaac aactttggta tagccggttc aaggacctga ccgacaagga
     1561 aggtgtgaag aacggagagc ttcagaatat ctactttgat gccggcatgc tttttctgtt
     1621 tgaaaagaaa atgggaaagg acaaactgac ggtcgttgat atcaaggcag gaaaagcctt
     1681 gtggacgact gatcgttatt cggatctcac ggaggataac atcgaataca tcccgcagtt
     1741 gggcgcattc ttcttttcat tgaagaatac tacggtcctg gtggatgcca agacaggcca
     1801 gcagcgctgg gaaaccgaca agttcaaagg ctcggtcggg gccttcctct atgacgattc
     1861 gcgcaatgag ttgttgatga tcaattacaa accctctcag ctggctgcct tgttcagcgg
     1921 attcaagaac cagttgatac gcatcaatgc cgccaacggg gaagtaaagt ggagcacgag
     1981 tttctttggc ttcgtggaac gaaaggtcct gacgcgtgaa gctttggcca gcctcaccct
     2041 gaaaggcggc aaggtattcc tggagctgga cggtattcag gtgtttgatc acgagacagg
     2101 tcaaatgctc tggagcgcgg tctatgaatc cgacgaggaa gcgcgcaggg gtcttttcaa
     2161 ccgaggcccc catggcggac gggtgatcaa gggtggtatc tacggcgcaa ttgccgagcc
     2221 attgtatacc aacgacggtg tatatgttgt gctgggtagc ggaaagctcc gcaccaagtt
     2281 catcgccaag tacgatctgg agtccggtaa gaaactctgg gaatcagaaa agatcaccgg
     2341 tgccggcgct atgcccaatc tgcacctgga agatggccgc ctggtggcac agatcggcgg
     2401 tattgtgaat aagcaaacga tcgaaaacaa ggtggagacc ggtcctaacg gtaccgtaac
     2461 gaccatctac tacaacaacg agtgggactt catgggcgga tacggcatta ttgccctgga
     2521 cccgtcttct ggtgccaaag tctggcggtc cgagaaattc gac
//
LOCUS       DAOTON010000397         2561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31564_length_2561_cov_3.849960, whole genome shotgun sequence.
ACCESSION   DAOTON010000397 DAOTON010000000
VERSION     DAOTON010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31564_length_2561_cov_3.849960"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="P5292_08260"
     CDS             complement(<1..851)
                     /locus_tag="P5292_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39157.1"
                     /translation="MANNSHTLFYLGAGASVPVLPVTSDILKEANNFINHIEDYKKKL
                     PNRPGITKPELGLLSNSLDDLLKDIRFLKAACEKHISIDTAAKKYLSQERVFEYEKIK
                     QSLTDLFSFVQFTKPVSTRYDAFIAALYDHRRSKLPEQVKVVSWNYDFQFELAFSEYY
                     NRYTYQHVADALNLTPHSNRSPSINSFFLYKMNGSATYFDNSNNEEFAPLFSKLNTDR
                     TIESVIEGIVHYRSRFYKNNIRFAFESNLDFEVLSQLRSSISNCVNLVVIGYSFPFFN
                     RDYDNFV"
     gene            1021..1314
                     /locus_tag="P5292_08265"
     CDS             1021..1314
                     /locus_tag="P5292_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39158.1"
                     /translation="MTHLANLIKLKANLTDEQAQLAAEAVMDHFKLKFPHILHAELDR
                     VAEGGEFGDTVREKFDDVRDKVEAVAKQAGAKAEELAKEVGEKLNEWFAKKKT"
     gene            1545..2072
                     /locus_tag="P5292_08270"
     CDS             1545..2072
                     /locus_tag="P5292_08270"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HRS39159.1"
                     /translation="MPVTRPFNFKQWIDEHRHLLKPPVMNQVVYQPNQDFIVMVVGGP
                     NSRKDYHYNETEEFFYQLEGDIVVKIIDDGKPVNIPIKEGEIFLLPARTPHSPQRGPN
                     TVGLVMEVQRRPGMKDGLQWYCESCHQLLHEEYFDLEDIVDQLAKAMTAFYEDKQKRT
                     CKHCGAVMEPPVPVK"
     gene            2126..>2561
                     /locus_tag="P5292_08275"
     CDS             2126..>2561
                     /locus_tag="P5292_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39160.1"
                     /translation="MSKKLTRKEKIAQQQQSAQPSKRQQAAELQQHQRSRNWAGLIVA
                     IVAFLVYANTFQHGWALDDWGVIPDNKLTKRGISAIPEIMSTTYRTGMDVSDHTLYRP
                     LSKATFAIEWSISPDNPSLGHLDNVILYALTCFLLFRVLLLCF"
BASE COUNT          711 a          622 c          618 g          610 t
ORIGIN      
        1 aatacaaagt tgtcgtaatc tcgattgaaa aatgggaagg agtagccgat tacaacaagg
       61 tttacgcagt tggaaataga tgatcgaagc tgggaaagta cttcaaaatc gaggttagat
      121 tcaaatgcaa atcgtatatt gtttttatag aacctggaac gataatggac aattccttca
      181 atcacgcttt cgattgttcg atccgtatta agtttggaga atagcggcgc aaattcctca
      241 ttatttgagt tgtcaaagta ggtagcgctc ccattcattt tatatagaaa gaaagagttt
      301 atcgaaggtg aacgattgga gtgtggagta agattaaggg catctgccac atgctgatag
      361 gtgtagcgat tgtaatattc agaaaacgcc aattcaaatt gaaaatcata gttccatgat
      421 acaactttta cttgttctgg cagtttagat ctgcgatgat cataaagcgc ggcgataaat
      481 gcgtcgtacc gtgtgctgac cggtttagtg aattgaacaa aagagaaaag atcggtgaga
      541 gactgtttta ttttttcata ttcaaatacg cgctcttgcg aaagatattt tttagcagca
      601 gtgtcaatgc ttatatgttt ttcacacgcc gcctttaaaa aacgaatatc cttcagtaag
      661 tcatcaagag aattagatag taaaccaagc tcgggcttag taataccggg tctattgggc
      721 agcttctttt tgtaatcttc aatatgatta ataaaattat tggcctcttt taatatgtcg
      781 ctcgtaactg gaagtacagg aacgcttgct cccgcgccga ggtaaaaaag ggtgtgtgag
      841 ttattagcca tatgctagtt ataataaaag atgtgattgg gagagaagtt gcggaataag
      901 taaaagccaa agcgcctata tatgccctga aactttccat agattaaaac caaacacagg
      961 gaggatgtaa agtggattaa ccatgtaaat ttattaactt taaacttcta atcgctcccc
     1021 atgacccacc tcgccaacct catcaagctc aaagccaacc tcaccgacga acaggcgcaa
     1081 ctggccgccg aggcggtgat ggaccatttc aaactcaagt tcccgcacat cctgcacgcg
     1141 gagctcgacc gggtcgccga gggcggcgaa tttggcgata ccgtgcggga gaagttcgac
     1201 gacgtacgcg acaaggtcga agccgtagcc aaacaagccg gcgccaaagc cgaagagctc
     1261 gccaaggagg tgggggagaa gttgaatgag tggtttgcga agaagaaaac ctgaccgggg
     1321 gttaatcgtt tcgggtttcg ggtttccggt ttcgagtcgt tttatgttgt aaaagttcga
     1381 tccagaccga ccccgagttc aatttaaagg tcaacagttt cactattccg gttccgagtt
     1441 gtttttggtt ttgaacagga aatcaacccg aaactggaaa cccgaaactg gaaactcgaa
     1501 accctaaacc cttaaattcg ccacgcagcc aacccccaac cgccatgccc gtaacccgcc
     1561 cgttcaactt caaacaatgg atcgacgaac accgtcacct gctcaagccg ccggtgatga
     1621 atcaggtcgt ctatcagccc aaccaggatt tcattgtcat ggtcgttggc ggccccaaca
     1681 gccgcaagga ttaccattac aacgagacgg aagagttttt ctatcagctc gagggcgaca
     1741 tcgtggtcaa gatcatcgac gacggcaagc cggtgaacat cccgatcaaa gaaggcgaga
     1801 tctttcttct gccggccagg actccgcatt ccccgcagcg aggtcccaat acggtcgggc
     1861 tggtcatgga agtgcagcga cgtccgggca tgaaggacgg cctccaatgg tattgcgaaa
     1921 gctgccacca attgctgcac gaggagtact ttgatctgga agacatcgtc gatcagttgg
     1981 cgaaagccat gacggccttt tacgaggaca aacaaaaacg tacctgtaag cattgcggag
     2041 ccgtcatgga gccgcctgta cccgtgaaat aatccgaata tgcccgccag tgcgggcata
     2101 ttgcttccca acctgttcca tccgcgtgag caagaaactt acccgtaaag agaagatcgc
     2161 gcaacaacag caatctgcgc aaccttccaa gcgccagcag gcagccgagt tgcaacaaca
     2221 ccagcgatcc cgtaactggg ccgggctgat cgtagcgatc gtcgcgttcc tggtctatgc
     2281 caacaccttt caacacggct gggcgctgga tgactggggc gtcattcccg acaacaagct
     2341 gaccaagcgg ggcatctccg ccattccgga gatcatgagc accacctacc gtacgggcat
     2401 ggacgtctcc gaccacacgt tgtatcgtcc gctctccaaa gcgaccttcg cgatcgagtg
     2461 gagcatttcc cctgacaacc cttcgctggg acacctagac aacgtcatcc tctatgcact
     2521 gacttgtttc ctgttgttcc gcgtattgct gctctgtttc c
//
LOCUS       DAOTON010000398         2559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31603_length_2559_cov_2.962460, whole genome shotgun sequence.
ACCESSION   DAOTON010000398 DAOTON010000000
VERSION     DAOTON010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31603_length_2559_cov_2.962460"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="P5292_08280"
     CDS             complement(<1..1051)
                     /locus_tag="P5292_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRS39161.1"
                     /translation="MKPSLPKGTRDFSPDEMLRRAHIFDTIRTVYQRYGFLPIETPAM
                     ENLSTLEGKYGEEGDKLLFRVLNSGQVLDQVKAAGSATGMEMLTEKGLRYDLTVPFAR
                     YVVMHQHELTFPFRRYQIQPVWRADRPQKGRYREFYQCDADVIGSDSLLNEVDLINII
                     HDVFDALELGVKILLNNRKVLSGIAGVLGEQERIIDITVAIDKLDKIGLDKVNEELKG
                     KGVSETAITRLRPLMELKGSNGDKLETLRGFLAASETGRKGIEELEQILSYLPNERQV
                     EIDITLARGLNYYTGAIIEVNSETDFVAKNDEFIALANGCAALVADKNPADVAALSAL
                     PMGEGTVESTRSALVG"
     gene            complement(1240..>2559)
                     /gene="ettA"
                     /locus_tag="P5292_08285"
     CDS             complement(1240..>2559)
                     /gene="ettA"
                     /locus_tag="P5292_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRS39162.1"
                     /translation="DEMNKLIERQGQVQDAIDAAGGWELDNKIEVAMDALRCPDPETN
                     VANLSGGEKRRLALCRLLLQEPDVLLLDEPTNHLDAESVLWLEQHLKQYAGTVIAVTH
                     DRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKSNRLAQEEKTESKRRKTLERELEW
                     VRMSPKGRHAKSKARIAAYDRLLNEDVKEREDKLELFIPPGPRLGSVVYEFENVTKSY
                     GDRVLFENLSFSLPPAGVVGVIGPNGAGKTTLFKLLMGLEQPDSGTIRRGDTVQVAYV
                     DQTHDDLVPGKTVFEIISGGTETMVVGGRQLNARAYVSKFNFSGTDQSKKLEVLSGGE
                     RNRVHLAIALKQGSNVLLLDEPTNDIDVNTLRALEEAIENFAGCAVIISHDRWFLDRV
                     ATHILAFEGDSQVYFFDGNFSEYEENKKQRLGDVQPHRIRYKSLVRS"
BASE COUNT          508 a          744 c          704 g          603 t
ORIGIN      
        1 tgcccaccag ggcggaacgg gtcgactcga ccgtaccttc acccatcggc agcgcggaca
       61 gcgcagcgac gtctgccggg ttcttgtcgg caaccagagc ggcacagccg ttggccagcg
      121 cgatgaactc gtcgttcttg gcgacgaagt cggtttcgga gttcacttcg atgatcgcgc
      181 cggtataata gttcaatccg cgcgccaggg tgatgtcgat ctcgacctgt cgttcgttcg
      241 gcaaatacga gagaatctgt tccaactcct cgatcccttt cctgccggtc tccgaagcgg
      301 ccaggaatcc acgcagggtt tccagcttgt cgccgttgct tcccttcaac tccatcagcg
      361 gtcgcagccg ggtaatggcc gtttcgctca cgccttttcc cttcagctcc tcgttgacct
      421 tatccaggcc gatcttgtcg agcttatcga tggcgacggt gatgtcgatg atccgctcct
      481 gctcgcccaa caccccggcg attccgctca ggactttccg gttgttcaac aggatcttaa
      541 caccgagttc cagggcatcg aagacgtcgt gaatgatgtt gatcaggtcc acttcattca
      601 ggagcgagtc gctgccgatc acatccgcgt cgcattggta aaactcccgg taccggccct
      661 tctgcggacg atcggcgcgc cataccggct ggatctgata gcgacggaac ggaaaagtga
      721 gctcgtgttg gtgcatcacc acgtagcggg caaagggtac cgtgagatcg taacggaggc
      781 ccttctctgt cagcatctcc atgcccgttg cgcttccggc cgctttcact tgatccagga
      841 cctgaccgga attcaacacg cgaaaaagga gcttgtctcc ctcttctcca tacttccctt
      901 cgagcgtgga tagattttcc attgccggtg tttcgatggg caggaatccg tagcgctgat
      961 acacagtacg aatggtgtcg aaaatatggg cgcgacgaag catctcgtcg ggagagaaat
     1021 cgcgtgtgcc tttgggtaaa ctcggtttca taatggtccg gaaccgtatg ggaattcggt
     1081 tttggatgat tgatctcaaa ggtagcggac gaggctggaa attcgcccct ggcttcgggg
     1141 agttatcctg cagaattcaa caatgaattc accgaactcc caatgaaaaa aggccggtct
     1201 gatggacacg gcctttccaa ggatgattgc agcaatcgat caggaccgca ccaggctctt
     1261 gtaccggatg cgatgcggct gaacatcgcc gaggcgctgt tttttgttct cttcgtattc
     1321 ggagaagttg ccgtcgaaga agtagacttg cgaatcgcct tcgaaggcca ggatgtgcgt
     1381 agctacgcga tcgaggaacc aacggtcgtg ggagatgatg accgcgcaac cggcaaaatt
     1441 ctcgatggct tcttcgagcg cccggagggt gttcacgtcg atgtcgttcg tcggttcgtc
     1501 gaggagtaag acgttgctgc cctgcttcaa cgctatcgcg aggtgaaccc ggttgcgctc
     1561 tccgccggaa agcacttcca gtttcttgct ctggtccgta ccgctgaagt tgaacttgga
     1621 aacataggcg cgggcattca actgtcgacc gcctacgacc atcgtttcgg ttccgccgga
     1681 gatgatctca aaaaccgtct tacccggtac caggtcgtcg tgcgtctggt ccacataagc
     1741 tacctgtacc gtatcgcccc gacgaatggt gccgctatcg ggttgttcga gacccatcag
     1801 cagtttaaag agcgtggtct taccggctcc gttcggaccg atgacaccga ccactccggc
     1861 cggtggcagg gagaagctca gattctcaaa caacacccgg tctccgtagg acttggtcac
     1921 gttctcaaac tcgtacacca cacttcccag gcgcgggccc ggcgggatga agagttcgag
     1981 cttgtcttcg cgctccttta catcctcgtt cagcaaacga tcataggcgg cgatacgggc
     2041 tttggatttt gcgtgacggc ctttcggcga catgcgtacc cattccaatt cccgctcgag
     2101 tgtcttgcgg cgcttgcttt ctgttttctc ttcctgagcc agacgattcg atttctgttc
     2161 gagccaggag gagtaatttc ccttccacgg aataccttca ccacgatcca gttcgaggat
     2221 ccagcccgct acgttgtcga ggaagtaacg atcgtgcgta acggcgatga ccgtaccggc
     2281 atactgcttg aggtgttgct ccagccagag gaccgattcc gcgtcgaggt ggttggtcgg
     2341 ttcgtcgagc agcagcacat ccggctcttg cagcagcaaa cgacaaagcg cgagacgacg
     2401 tttctcaccg cccgacaggt tggccacatt cgtttccgga tccggacagc gcagcgcatc
     2461 catggcgacc tcgattttgt tgtcgagctc ccatccccct gcggcatcga tggcatcttg
     2521 tacctgccct tgacgctcga tgagtttatt catctcatc
//
LOCUS       DAOTON010000399         2557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31627_length_2557_cov_3.762990, whole genome shotgun sequence.
ACCESSION   DAOTON010000399 DAOTON010000000
VERSION     DAOTON010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31627_length_2557_cov_3.762990"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..811
                     /locus_tag="P5292_08290"
     CDS             50..811
                     /locus_tag="P5292_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39163.1"
                     /translation="MRRHLLLIFLLPATLVLAQTPEADLKKALVLKDQKRWEESLAIF
                     QQLLKSDSTRVPYLTNTAYLLCKAGNRETVDEKRQSYFRKAEYLSRKAIALQATDAEA
                     HYNYALALGRINENASSKQKIANAKVIRNECDLALKYDPKLAGAWHILGRWHRTVAGF
                     NFVEKAMINTLFGGVPEGGSYESAIDCFSKAVQLEPEVMLHKIELAQTYADRNQGKDD
                     VLARVWCKKVLDLTARDDDDRDCVSRAKALLAKLD"
     gene            complement(808..2277)
                     /locus_tag="P5292_08295"
     CDS             complement(808..2277)
                     /locus_tag="P5292_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRS39164.1"
                     /translation="MGVIKRQGIKNTITGYLGIVIGFVNLILIQPHFLTKEELGLTRI
                     LYSFSLIVAMFVPMGISNAIIRYFPTFKDEQRKHHGFFAFASLFPLLGFLLAGAILWI
                     FRDFFLNQYRRESPLFLEYFDWVFPLIFFNSFISVLTAWCQANLKSTIPAFLNDVVIR
                     VLTIGLVSIYFLRWLDLDGFIAAFVALYGFQMVTLLVYIFQFDRPGFRIDWQVFREKK
                     FTELIRFGLLLWFASVASIGLKYFDSIMIGKFMPLSFVGIYTIAAFIPTVIEAPLNAF
                     EKIAASKISFAWARNDRQEIYSIYHKSAVYLFLLGGWLFLMINANTTALFTFLPEGYE
                     LGAPVILIISVATVFNMATGLNAPILFNSERYRWGAFFLIFLAIAIVVLQMLLIPAYG
                     LFGAALATSLASIAYNLLLLISVWKFFGLQPFDQRNLKVLMILLLTFAIALLIPHLQN
                     RWMDIAFRCSVLSLCYGGAIYAWKVVPEFHRYLPWERKP"
     gene            complement(2291..>2557)
                     /locus_tag="P5292_08300"
     CDS             complement(2291..>2557)
                     /locus_tag="P5292_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRS39165.1"
                     /translation="KKIVHRHIPRELMERPKMGFGVPLVDWLKTDLRPFLEHALREGS
                     FDHGLLDPRQVGRLKSAYLAGRLENFERLWYVLMFQTWYDRWMR"
BASE COUNT          691 a          667 c          716 g          483 t
ORIGIN      
        1 gaaaacgcca aacgccaggt caaacgtgta ttctgaaaac gtccacgaca tgcgccgcca
       61 tctactgctc atttttcttc ttcccgctac gctggtgctt gctcaaactc ccgaagccga
      121 tctcaaaaag gcgttggtac tgaaagacca gaaacgctgg gaggaatcac ttgccatctt
      181 ccagcaactg ctcaaatccg attcgacgcg ggtcccttac ctcaccaaca ccgcttatct
      241 actgtgcaaa gccgggaatc gcgagacggt tgatgaaaaa cggcagtcat atttccgcaa
      301 agcggaatac ctttcccgaa aagcgatcgc cttgcaggct acggatgccg aagcgcacta
      361 caactacgcg ctggccctgg gtcgcatcaa tgaaaacgct tccagcaagc agaagatcgc
      421 caacgcaaag gtgatccgca atgaatgcga cctcgctttg aaatacgatc cgaaactggc
      481 gggcgcctgg cacatcctgg gccgctggca ccgcaccgtg gccggtttca attttgtgga
      541 aaaggcgatg atcaacaccc tcttcggcgg tgtaccggag ggcgggtcct acgaatcggc
      601 gatcgattgt ttcagtaaag ccgtgcaact ggaacccgaa gtcatgttac acaagatcga
      661 gttggcacaa acgtatgccg atcggaatca aggcaaagac gacgtgctgg ccagggtgtg
      721 gtgcaagaaa gtactggatc ttacggcgcg ggatgatgat gaccgcgatt gtgtcagccg
      781 ggccaaagcc ctgttggcga aactcgatta aggctttctc tcccagggaa ggtaccggtg
      841 gaattccggc accactttcc acgcgtaaat cgctccgcca tagcacaggc taaggaccga
      901 acatcggaat gcgatatcca tccaccggtt ttgcaggtgc gggatcagca aggcgatggc
      961 aaaggtcagc aagaggatca tcagcacctt caggttgcgt tgatcaaacg gttgcaagcc
     1021 gaagaacttc cagactgaaa tgagcaacag caggttgtac gcgatcgagg caagagaggt
     1081 ggcaagcgca gcgccaaaca aaccataagc cgggatcagc agcatttgca acaccacgat
     1141 ggcgatagcc aggaagatga ggaagaaagc tccccagcga taacgctcgg agttgaacag
     1201 gatcggggca ttcagcccgg tggccatgtt gaagaccgtg gcaacggaga tgatcaggat
     1261 gacgggcgcg cccaattcgt aaccttcagg cagaaaggta aacaaggcgg tggtgttcgc
     1321 gttgatcatc aggaataacc agcctccgag gaggaacagg tagacagcgg acttatggta
     1381 gatcgaatag atctcctggc ggtcgttgcg cgcccaggcg aaggatatct tggaagccgc
     1441 gatcttttca aacgcgttga gcggcgcctc gatcacggtc gggatgaacg cggcaatggt
     1501 gtaaatacct acaaacgaca acggcatgaa cttaccgatc atgatgctgt cgaagtattt
     1561 caaaccgatt gaagcgacgg acgcgaacca gagcagtaat ccgaaccgga tgagctcggt
     1621 gaactttttt tcccggaaca cctgccagtc gatccggaac cctgggcgat cgaattgaaa
     1681 tatgtaaacc agcaaagtca ccatctggaa tccatacaag gcgacgaagg cggcgatgaa
     1741 tccatccagg tcgagccagc ggaggaagta aatgctgacc agaccgatgg tcaatacgcg
     1801 gatcacgaca tcgttcagaa acgcggggat ggtagacttc aggttggcct ggcaccaggc
     1861 ggtcagaacc gagatgaacg aattgaaaaa gatcagcggg aacacccagt cgaagtactc
     1921 cagaaaaagc ggcgactccc gccggtattg gttcaggaaa aagtcgcgga agatccaaag
     1981 gatggcgcct gccagcagaa agcccagcag tggaaacaag ctggcgaatg cgaagaaacc
     2041 atggtgcttc cgctgttcat ccttgaaggt cgggaagtac cggatgatgg cattgctgat
     2101 gcccatcggg acaaacatgg cgacgatcag cgaaaacgaa tacaggatcc gggtaagccc
     2161 cagttcttcc ttggttaaga agtggggttg aatcaggatc aggttaacga aaccgatgac
     2221 aataccgagg taaccggtga tcgtgttctt gatgccttgt cgtttgatga ctcccatcgg
     2281 ttcctttcga ttaccgcatc cagcggtcgt accaggtttg aaacatgaga acgtaccaca
     2341 accgctcgaa attctccagt cggccggcga ggtaagccga cttcaaccga ccgacctgcc
     2401 tgggatccag caagccgtga tcgaacgatc cttcgcgcag agcatgttcg aggaatggac
     2461 ggagatccgt tttcaaccag tcgaccaggg ggacgccaaa acccatctta gggcgctcca
     2521 tcagttcacg cggtatgtgc cggtggacga tcttctt
//
LOCUS       DAOTON010000400         2537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31941_length_2537_cov_2.500403, whole genome shotgun sequence.
ACCESSION   DAOTON010000400 DAOTON010000000
VERSION     DAOTON010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31941_length_2537_cov_2.500403"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..2506)
                     /locus_tag="P5292_08305"
     CDS             complement(23..2506)
                     /locus_tag="P5292_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HRS39166.1"
                     /translation="MSATTYTLRELADIAGAELLLNAPDRDGFTELLTDSRKLVHPEE
                     TVFFALSGERHDGHRFIHELSGQGVKNFVVSRLTDALRDLPANFALVPDTLVALQKLA
                     SFHRKRFNIPVIGITGSNGKTIVKEWLYQLLRTDKNIVRSPKSYNSQIGVPLSVWGME
                     PEHALGIFEAGISRTGEMARLAAIIEPTIGLITNIGAPHDENFENRIQKVEEKLRLFP
                     SVHTLIYCRDFPEIHAAVGQLPFRDPACKRFTWSRKVKADLQIGRITRQDDDTEIQGI
                     WQDRFVRIRIPFTDDASIENAIHCWAVLLVIGLSQESIAERMQYLSPVAMRLELKNGI
                     NNCSVINDSYNSDLGSLTIALDFICQQKQHAKRTVILSDILQSGRNEETLYEEVAALL
                     QARKIDRLIGIGDALLRQRELFRMPAQFYRSTEAYLREVTTADFRDETILLKGARPFG
                     FEQIGKFLQQKAHETVLEINLDALVHNLNYYRSRLRGSTKIMAMVKAASYGSGSYEIA
                     NTLQFHHVDWLAVAYTDEGVELRKAGIGLPIMVMNPEVQNFEAMLRYRLEPEIYSFRL
                     LGQLVDMLRKHDGDPFRIHVKLDTGMHRLGFEEKDIHELAVHLRNHRNIRVQSVFTHL
                     VASDESEHDGFTRLQFERFERMSAVLEEALPQPFLRHALNSSGIFRFNEAQYDMVRLG
                     IGLYGIASTSHEQQQLQQVATLRTTVSQIKHVPLNETVGYSRKGRLKRDSVIATVAIG
                     YADGINRRLGNGIGKFLVNGRLAPIVGNVCMDMCMLDVTDIPVREGDEVIVFGPELPV
                     TKLAESLGTIPYEVLAGISQRVKRVYFQE"
BASE COUNT          525 a          712 c          708 g          592 t
ORIGIN      
        1 ctttttctga gccggcactt gtttattcct gaaaatagac ccgctttacc cgctgggaaa
       61 taccggcgag tacttcgtat gggatggtac ccagcgactc tgccagtttt gtcacgggca
      121 actccggacc gaagacaatg acctcgtcgc cttcgcgcac gggaatgtca gtaacatcca
      181 gcatgcacat gtccatgcac acattgccaa cgatcggcgc caggcgtccg ttgacgagga
      241 acttcccgat gccgttgccc agtcgacggt tgatgccgtc cgcataaccg atggcaacgg
      301 ttgcgatcac cgagtccctt ttaagacgac cttttctgga atacccgacg gtttcgttca
      361 acggcacgtg tttgatctgc gacacggtcg tgcgcagggt cgcgacctgc tgcaattgct
      421 gttgctcatg gctcgtagag gcgatgccgt agagaccgat ccccagtctg accatgtcgt
      481 actgcgcttc gttgaatcgg aaaataccac tggagttgag ggcgtgccgg agaaacggct
      541 gcgggagcgc ttcttccaac acggcgctca tgcgctcgaa tcgctcgaac tgaaggcggg
      601 taaatccgtc gtgctccgac tcgtcgctgg cgaccaggtg ggtgaaaacg gactgcaccc
      661 ggatattcct gtggttccgc agatgaacgg ccaactcatg gatgtctttc tcttcaaacc
      721 ccagacggtg catgccggta tccagtttca catggatccg aaaaggatcg ccgtcgtgtt
      781 tgcgcagcat gtcgaccaac tgacccaaca ggcggaagct gtaaatctcc ggctccaggc
      841 ggtagcgcaa catcgcttcg aaattttgca cttccggatt catgaccatg atcggcaaac
      901 cgattccggc tttccgcagc tctacccctt catcggtgta cgctaccgct agccagtcga
      961 catgatggaa ctgcaaggta ttggcgatct cgtagctgcc gctgccatac gaggcggctt
     1021 tgaccatcgc catgatcttc gtgctgccgc gtagccggga ccggtaataa ttcaggttat
     1081 gcacgagcgc atcgagattg atctccagca ccgtctcgtg cgccttttgc tggaggaact
     1141 tcccgatctg ttcgaatccg aaagggcgtg cgcccttcag taaaatagtc tcatcgcgga
     1201 aatcggccgt ggtcacttcc cgcaggtagg cttcggtaga gcgatagaat tgcgccggca
     1261 tgcggaacaa ttcgcgctgc cggagcaacg catcgccgat cccgatcagc cggtcgatct
     1321 tgcgggcttg caggagcgct gccacttcct cgtagagcgt ttcttcattg cgcccgcttt
     1381 gcaggatatc ggacaggatc accgtccgct tcgcgtgctg cttctgctga cagatgaaat
     1441 ccaacgcgat cgtcaacgag ccgagatcgg aattatagct gtcgttgatg accgaacaat
     1501 tgttgatgcc attcttcagc tccaggcgca tcgccaccgg gctgaggtat tgcatgcgtt
     1561 ccgcaatcga ttcctggctg aggccgatga ccagcaacac ggcccagcag tgaatggcat
     1621 tctcgatgga cgcatcgtcg gtaaacggaa tgcggatgcg tacgaaccgg tcctgccaga
     1681 taccctggat ctccgtatcg tcgtcctgac gggtgatacg accgatctgc agatcggctt
     1741 tgaccttccg cgaccaggtg aaacgcttgc aggcaggatc gcggaatggc aattgcccta
     1801 ccgcagcatg gatctccggg aaatctcgac aatagatcaa ggtgtgaacg gatgggaaga
     1861 gccggagctt ctcttccact ttttggatcc tgttctcgaa attttcgtcg tgcggggcgc
     1921 cgatattggt gatcaatccg atcgtcggct caatgatcgc cgccagtcgg gccatttcgc
     1981 cggtccggga aatacccgct tcaaagattc ccagggcgtg ttcgggctcc attccccaga
     2041 ccgacaaggg tacgccgatt tgtgagttat agcttttcgg actacgtaca atgtttttat
     2101 ccgtgcgcag caattgatat agccattcct tgacaatggt ctttccattg ctgccggtga
     2161 tgccgatgac gggtatattg aatcgcttac ggtgaaagga tgccagtttc tggagcgcga
     2221 ccagggtatc gggcaccaat gcaaaattgg ccggaaggtc ccgcaaggcg tccgtaagcc
     2281 ggctgacgac aaagttcttt acaccttgtc cggatagttc atggataaag cgatggccat
     2341 cgtgccgttc gccactgagg gcgaagaaaa cggtctcttc gggatgaacg agttttcggc
     2401 tgtcggtcag gagttcggta aatccgtcgc ggtccggtgc gttcagcaac agttccgctc
     2461 ccgcgatatc agcaagttcc cggagtgtat acgtcgttgc gctcatgcat caggttccgg
     2521 gcgtgttctt gagttct
//
LOCUS       DAOTON010000401         2535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31970_length_2535_cov_3.019355, whole genome shotgun sequence.
ACCESSION   DAOTON010000401 DAOTON010000000
VERSION     DAOTON010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31970_length_2535_cov_3.019355"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="P5292_08310"
     CDS             complement(<1..413)
                     /locus_tag="P5292_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39167.1"
                     /translation="MKYILTFLCALALNGASAQFSFQNINLLSNFDDPTVPAEPVYGI
                     RYQSCWGWADPLTDREYGIIGSSEGTYIIEVTDPGNPVQRDYIPGRSNQRIWHEYKTV
                     GNYLYIISDGGGNSLQIVDLTTLPDSGHVVYDGTN"
     gene            complement(441..1520)
                     /gene="lpxK"
                     /locus_tag="P5292_08315"
     CDS             complement(441..1520)
                     /gene="lpxK"
                     /locus_tag="P5292_08315"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRS39168.1"
                     /translation="MSGWRTLLLPFSLLYGLVTIVRNWLYDKRLLPSYSSPLPTIGVG
                     NLTTGGTGKTPHIEYLVRLLRPHYRVATLSRGYGRKTKGFMIVSNPSGTDMIGDEPMQ
                     YYSKFSDILVSVGEDRAAALQRLERHHPKPHVVLLDDVFQHRAVRPGHQILLIEYDRI
                     MQTELLLPAGDLREVKSGQRRADTIIITKSPAILVPIERKRILEHLQPLEHQQVFFTY
                     YRYGDFTRLHAKPGGMMMGSQYYLEKRFTILMVTGIAHPATLIEHLRRQSDKLEMMLF
                     PDHHEFTEKDIRRIQETFDQIANPNKILVTTEKDAMRFRNPRIDVLTRNMPIFYIPIE
                     VAFHQDGEKFDQTITRYVTAARKPS"
     gene            1655..>2535
                     /locus_tag="P5292_08320"
     CDS             1655..>2535
                     /locus_tag="P5292_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRS39169.1"
                     /translation="MAVTIGDVAQCLESFAPLRYQESYDNAGLLVGDRNQPVNGVLIC
                     LDSTEAVLEEAIRHGCNLIVAHHPIVFSGLKRFTGASYIERVVMGAIRNNIAIYAAHT
                     NLDNVQEGVNARIAARIGLLDTRILSPKKGLLRKLVTFVPHAQADHVREAMFQAGAGH
                     IGDYDACSFNVEGTGTFRGGAGTNPAVGKPGENHREPETRIELIFPAYRESAILKALF
                     SAHPYEEVAYDIYSLENQYNEVGSGLIGRLPEPMDGRIFLLDLKKRMGAEVVRHTQLL
                     DRKVQKIAVCGGSGSFL"
BASE COUNT          550 a          623 c          759 g          603 t
ORIGIN      
        1 atgttcgtgc cgtcgtacac gacatgtccg gagtcgggga gcgtggtcag gtccacgatc
       61 tgcagactgt tgccgccacc gtcgctgatg atgtagagat aatttccgac cgtcttgtat
      121 tcgtgccaaa tgcgctggtt cgaacgaccg ggaatgtagt cgcgctgaac cggattgcca
      181 ggatcggtaa cttcaatgat gtaagtgcct tcggaagatc cgatgatgcc gtactcccgg
      241 tcggtcagcg gatcggccca accccaacaa ctctggtatc ggatcccgta gacgggctcg
      301 gcgggcactg tcgggtcgtc gaagttgctg agcaggttga tgttctggaa gctgaactgg
      361 gcactggcgc cattcagcgc cagcgcgcag agaaatgtca ggatgtattt catgatttgg
      421 aggagttgat gctatcggaa tcaggagggt tttctggctg cagtgacgta tcgggtaatg
      481 gtctggtcga atttttcacc atcctgatgg aaggcgactt cgatcgggat gtaaaagatg
      541 ggcatgttcc gcgtgagcac gtcaattctt gggttgcgga aacgcatggc gtctttttcc
      601 gtggtgacca ggatcttgtt cggattcgcg atctggtcga aggtttcctg gatgcggcgg
      661 atatcctttt cggtgaattc gtgatggtcc ggaaagagca tcatctccag tttatccgac
      721 tgtcgccgga ggtgctcgat caatgtcgcc gggtgggcaa tgccggttac catcaggatg
      781 gtaaagcgct tttcgaggta atactgcgaa cccatcatca tgccgccggg cttggcgtgc
      841 aagcgggtga aatcaccgta acggtaataa gtgaaaaaga cctgctggtg ctccagtggt
      901 tgcaaatgtt ccaggatcct tttccgttcg atcggtacca ggatggccgg tgacttggtg
      961 atgataatgg tgtcggcccg tcgttgacct gatttcactt cgcgcagatc tccggccggc
     1021 aacaggagtt cggtctgcat gatccggtcg tattctatta acaggatctg gtgacccggt
     1081 cgtacggccc gatgctggaa cacatcgtcg agtaacacga catgcggctt ggggtggtgg
     1141 cgttctaaac gttgaagcgc agccgcccgg tcttcgccga cggaaacaag aatatcggag
     1201 aacttggaat aatactgcat cggctcgtca ccgatcatgt cggttccgga cggattggat
     1261 acgatcatga atcctttcgt cttccggcca tagccccggc tgagcgtggc gacgcggtag
     1321 tgtggccgca gcagacggac gaggtattcg atgtgtgggg tcttcccggt tccgccggtg
     1381 gtcagattac ccacgccgat cgtcggtaag ggcgaggaat atgatggcag gagccgcttg
     1441 tcgtagagcc agtttcgaac gatcgtcacc aggccgtaca agagcgagaa tggtagtaaa
     1501 agagttctcc agccggacat gccgggcaaa ttacgcaaat tagagcgagg tgtatagctt
     1561 atagccttgg tcgggtcagg ctcgtgtcag gagttgctga tcgtcccaag ccggcgcatt
     1621 tcgtaagttt gaaccttcaa gttctcgaga aactatggcc gttacgatcg gagatgttgc
     1681 gcaatgcctg gaatccttcg ccccgctgcg gtatcaggaa agttacgaca atgccggatt
     1741 actggttggt gatcggaacc agcccgtcaa tggcgtcttg atttgcctcg atagtaccga
     1801 agctgttctg gaggaagcga tccggcacgg atgtaacctg atcgttgcgc accatccgat
     1861 cgtcttttcc ggcttgaaac gatttaccgg cgcgtcctac atcgaacgag tcgtaatggg
     1921 cgcgatccgg aacaacatcg cgatctacgc tgcgcatacc aacctcgata atgtgcagga
     1981 aggcgtgaac gcacggatcg ccgcacggat cggtttgctc gatacccgga tcctctcacc
     2041 aaagaagggt ttactcagga agctggtcac cttcgttccc cacgcacagg cagatcatgt
     2101 tcgcgaggca atgtttcagg ccggcgccgg gcacataggg gattacgatg cctgcagttt
     2161 caatgtggaa ggcacaggca ccttccgggg cggagcggga accaatccgg cggttggaaa
     2221 gcctggtgaa aaccaccgcg agcccgaaac gcggattgag ctcatctttc ccgcataccg
     2281 ggaatcagcg attttaaaag cactcttctc agcgcatcca tacgaggaag tcgcttacga
     2341 catttattcg cttgaaaatc agtataatga agttgggtca gggcttattg gccgtcttcc
     2401 ggaacccatg gatggccgca tatttcttct ggatttgaaa aaaaggatgg gagcggaggt
     2461 tgtccggcat acgcagcttt tggatcgaaa agtccaaaaa atagccgttt gcggtggttc
     2521 cggtagtttt ttgtt
//
LOCUS       DAOTON010000402         2535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_31972_length_2535_cov_2.703629, whole genome shotgun sequence.
ACCESSION   DAOTON010000402 DAOTON010000000
VERSION     DAOTON010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31972_length_2535_cov_2.703629"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="P5292_08325"
     CDS             complement(<1..597)
                     /locus_tag="P5292_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRS39170.1"
                     /translation="MKNPTPRILALIAALAASVVVLAAEYVTTVSTYFQKNWLSMLVV
                     FVITFVTTFLIFSYLLEVFIYRKIKLIYKTISTFKDSKEKALERVDLDADILGNVSKE
                     VMGWEKDRTEEIEQLKRQELFRREFLGNVSHELKTPLFNIQGYIHTLLEGGINDPEVN
                     LHYLQRASKSADRLCLIVEDLEAISKLESGELVLDQRTF"
     gene            complement(642..1337)
                     /locus_tag="P5292_08330"
     CDS             complement(642..1337)
                     /locus_tag="P5292_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS39171.1"
                     /translation="MSDHRYKILLVDDEPDILEFMEYNLKKENYTVFKAGNGRDAIRI
                     AKTERPDLIILDIMMPEMDGIETCRTLREMPEFRKTMIAFLTARNEDYSQIAGFDVGA
                     DDYITKPIKPRVLISRIGALFRRYDPTGASSDAKAQVLTIGDLQIDREQYLIQKGGEK
                     IELPRKEFELLALLASKPGKVFTREEILERVWGNDVIVGDRTIDVHVRKLREKLGEEL
                     IRTVKGIGYKFDF"
     gene            complement(1424..>2535)
                     /locus_tag="P5292_08335"
     CDS             complement(1424..>2535)
                     /locus_tag="P5292_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS39172.1"
                     /translation="DAHIMASHAERMKQSRCFIVSAKQAEAMQGALKPYQDAMTCVTC
                     HNPHVSVRETGSGVFNAACNGCHGDRDKVQCTASVTERNREADNCVGCHMPKNGTLDI
                     PHVVTTDHRIRKPAQKREVERIREFVRLACINNPEPDQRSRAVAYLNQFEKFTRDGQF
                     LDSARALLADASPADVQRNFSSLVRWAYLKEDYQQLLRWAESDAGKGQSELTAVDRAW
                     TDYRIGVAYRETGLKEAAATAFRRAIRLLPLQLDFRNELASVLIDLGKFGEAREQLEF
                     VLRENPEHLSAWVNLGFLSLSALHDLKAAERCYRTALALEPDHVQALINLAGTQVLSG
                     DVLAAKRLLQRALVLEPSNQQARIRIKALQETSTR"
BASE COUNT          565 a          740 c          617 g          613 t
ORIGIN      
        1 gaaggtccgc tggtctagca ccagttcacc actttcgagt ttggaaatgg cctccaggtc
       61 ttccacgatc agacacaagc gatccgctga tttgctggcg cgttgcagat agtgcaggtt
      121 gacttccgga tcgttgatac cgccttccag cagcgtatgg atgtatccct ggatgttgaa
      181 gagcggggtc ttcagctcat gcgagacgtt ccccaaaaat tcccgccgaa aaagctcctg
      241 tcgtttcagt tgctcgatct cttccgtacg gtccttctcc cagcccatca cttccttgct
      301 gacattccca aggatatcgg cgtccagatc gacgcgctcg agcgcctttt ccttactgtc
      361 tttgaatgta ctgatcgtct tatagatcag cttgatcttt cgataaatga agacctccaa
      421 taaataggag aagatcagaa aggtggtgac aaaggtgatg acaaatacca ccaacatact
      481 caaccagttc ttctgaaagt acgtggagac cgtggtgacg tactcggccg ccagaacaac
      541 aaccgacgcc gccagggcgg cgatcaacgc gagtatgcgg ggtgtagggt tcttcaagct
      601 acgttcaatt gccccccaaa gtacgaaaag cggtttgttg ctcagaaatc aaatttgtaa
      661 ccaatccctt tgacggttcg gatcagttct tcgccaagct tctcccgcaa tttgcgcaca
      721 tgaacgtcga tcgtccggtc acctacgatc acgtcattac cccacacacg ttccaagatc
      781 tcttcgcgcg taaacacctt cccaggcttg gaagccagta aggcgagcaa ttcgaattcc
      841 ttcctgggca gctcgatctt ttctccgcct ttttggatga ggtattgttc gcgatcgatc
      901 tgcaggtcgc cgatcgtcaa cacctgggcc ttagcatccg aagacgcgcc ggtaggatcg
      961 taacgacgaa acaacgcgcc gatcctgctg atcaacaccc gcggcttgat cggcttggtg
     1021 atataatcat cggctcctac atcaaagcct gcgatctggg aataatcttc gttacgcgcc
     1081 gtcaggaacg cgatcatggt cttccggaac tccggcattt cccggagcgt tcggcaagtc
     1141 tcgatgccat ccatttctgg catcatgata tcgagaatga tcaggtcggg acgttccgtt
     1201 ttggcgatcc gaatggcatc ccgcccgttt cccgccttaa agaccgtgta attctctttt
     1261 ttcaggttgt attccatgaa ttcgagaatg tccggttcgt cgtctaccaa caggatctta
     1321 taccgatgat cgctcataat ttcgtttccc gaaggtactc aggccatcca ttcggcttgt
     1381 taagcgaatg taaagaaaag ataaagccag cgttgcggat atcttaacgc gtactggttt
     1441 cctgcagcgc ctttatcctg atccgagctt gttgattaga tggttcgagc accagcgcgc
     1501 gttgcaataa gcgttttgcc gcaagcacat cgcccgacaa tacctgcgtg ccagccaggt
     1561 tgatcaaggc ctgtacatgg tccggttcga gggccagcgc ggtccgataa catcgctcgg
     1621 ccgccttcag atcatgcagc gccgacaagg aaaggaatcc aagattcacc catgcggaca
     1681 ggtgctccgg attttcgcgc agcacgaact ccaactgctc cctggcctct ccgaactttc
     1741 caaggtcgat caacactgat gccaattcgt tcctgaaatc cagctgtagc ggcaggagtc
     1801 ggatcgcgcg acggaaagcg gtcgctgccg cttcctttaa cccggtctcg cggtaagcca
     1861 ccccgatacg gtaatcagtc caggcccgat caacagccgt cagctccgat tgtcctttcc
     1921 cggcatccga ctccgcccac cgaagaagtt gctgataatc ctctttcagg taggcccatc
     1981 gcacaagcga ggaaaaattc cgttgaacat ccgcaggtga agcatccgcc aacaaggcac
     2041 gggcgctgtc caggaactga ccatcgcgcg tgaatttttc aaactgattc aggtaagcga
     2101 ctgccctgct ccgctggtcc ggttccggat tgttgataca ggctaatcgt acgaactccc
     2161 gtattcgttc cacctcccgt ttttgcgccg gcttccggat gcggtggtcc gtcgtgacaa
     2221 catgcggaat atccaacgtg ccgttcttcg gcatgtgaca accgacacag ttatccgctt
     2281 cacgattccg ctccgtcacc gaggcggtac actgcacttt atcccgatca ccatgacaac
     2341 cattgcaggc ggcgttgaac actccgctgc ccgtttcgcg aacgctcaca tggggattgt
     2401 gacaagtcac acaggtcatc gcatcctgat agggcttcaa cgctccctgc attgcttcag
     2461 cctgcttggc cgaaactata aaacaccggc tttgcttcat gcgttcggca tgggaagcca
     2521 tgatgtgcgc gtctt
//
LOCUS       DAOTON010000403         2525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_32148_length_2525_cov_2.664372, whole genome shotgun sequence.
ACCESSION   DAOTON010000403 DAOTON010000000
VERSION     DAOTON010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32148_length_2525_cov_2.664372"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="P5292_08340"
     CDS             complement(<1..621)
                     /locus_tag="P5292_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005652735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HRS39173.1"
                     /translation="MFESLSDKLEKAFKLLKGEGRITEINVAETLKEVRKALLDADVN
                     YKVAKQFTDTVKEKALGQDVLKSVSPGQLLVKIAHDELAQLMGGSKSEVDLNGNPTII
                     LMSGLQGSGKTTLSGKLANFLKSKKQKLPLLVACDVYRPAAIDQLKVLGEQIEVPVYS
                     EPGNQDPITIAQNGIRYAKEHNRNVVIIDTAGRLAIDEEMMDEIARI"
     gene            complement(705..1466)
                     /locus_tag="P5292_08345"
     CDS             complement(705..1466)
                     /locus_tag="P5292_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRS39174.1"
                     /translation="MRITFLGTGTSQGVPMIGCTCRVCTSPDPKDKRLRTSVLVEGPS
                     GAVVIDSGPDFRQQMLRESVRSLNAVVFTHEHKDHIAGLDDVRAYNYLSGKPMEVYAT
                     ERVQEALKREFHYVFSNVQYPGIPRIELSTIHQVPFEVAGMVFQPIEVMHMNLPVLGF
                     RIGDFTYITDANFIPEPELEKIRGSRILVLNALRREKHVSHYTLQEAVDLIRSVQPEQ
                     AFLTHLSHQMGRHEEINAELPAGIQCAYDGLKLDC"
     gene            complement(1475..1765)
                     /locus_tag="P5292_08350"
     CDS             complement(1475..1765)
                     /locus_tag="P5292_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39175.1"
                     /translation="MLKLTQATLDKLEELLVLGGYKVRHEKGNFKSGSCIVEASKIIV
                     LNKFSPVEAKVSYLIEAIQSLELDESLLDDRYRKLLADARQMKLQPSEQPAS"
     gene            1884..>2525
                     /locus_tag="P5292_08355"
     CDS             1884..>2525
                     /locus_tag="P5292_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HRS39176.1"
                     /translation="MILAVTKETKDRERRVALTPEVVQTLIKAGYEVRVQSGAGLNAY
                     YEDSAYEKAGAQVQADRQQVLQSADVLLNVNPPSPDDIAKLKKGAVVISFLYAAINPA
                     VVEACVRQGVSAFSIDAIPRISRAQKMDALSSQANLAGYKAVLLGATALGKIFPLLMT
                     AAGTLKPSKVVIMGAGVAGLQAIATAKRLGAIVEVSDIRPETKEQVESLGGKFI"
BASE COUNT          564 a          679 c          674 g          608 t
ORIGIN      
        1 gatccgggcg atctcgtcca tcatctcttc gtcgatcgcc aggcgacccg cggtatcgat
       61 gatgacgacg ttacggttat gctccttcgc gtaccggata ccgttctgcg caatggtaat
      121 ggggtcctga ttgccgggct cgctgtaaac gggcacctcg atctgttcgc ccagcacttt
      181 gagctgatca atagcggcag ggcggtaaac gtcgcaggca accagtaaag ggagtttctg
      241 cttttttgac ttaaggaagt tggccagctt gccggagagc gtcgttttac cggaaccctg
      301 gagacccgac atcaggatga tcgtcgggtt gccgttcagg tcaacttccg atttggagcc
      361 ccccatcaac tgggcgagtt catcgtgcgc gatcttgacc aacaattggc cgggagaaac
      421 ggatttgagt acgtcctgac caagtgcttt ttccttgacc gtatccgtga attgcttggc
      481 gactttatag ttgacgtccg cgtcgagcag cgccttccgc acctccttca gggtttccgc
      541 cacgttaatt tccgtgatcc ggccttcccc tttgagaagt ttaaaagcct tttcaagttt
      601 atcgcttaag ctctcaaaca tgattgatat tcaattagtt atgtaattcc agtggaagtt
      661 gaaattcccc cgcaaagata gcattcgatc cgggacttcc cgactcagca gtccagtttt
      721 agtccgtcat aggcacactg aataccggcc ggaagttccg cattgatctc ctcgtgccgg
      781 cccatttggt gcgacaggtg ggtcagaaag gcctgttccg gttgaacgga acgtatcaaa
      841 tcaaccgctt cctgcagggt ataatgcgag acatgttttt cccgccggag cgcgttcaag
      901 accagtatgc gtgaaccacg gatcttctcc aactccggtt ccgggatgaa attggcatcc
      961 gtgatgtagg tgaaatcgcc gatcctgaaa ccgagcaccg gcaggttcat gtgcatgacc
     1021 tcgatgggct ggaagaccat tcccgcaact tcgaatggta cctggtgaat cgtcgagagt
     1081 tcgatccttg gaatacccgg atattggaca ttggaaaaga cgtagtgaaa ctcccgtttc
     1141 agcgcctctt gtacccgctc cgtcgcatac acctccatgg gtttaccact gaggtaattg
     1201 taggctcgga catcgtcgag cccggcgata tggtccttgt gttcgtgcgt gaagactacc
     1261 gcattgagcg aacgtaccga ttcacgtaac atttgctgtc ggaaatcggg gccggaatcg
     1321 atgactacgg ctccggacgg gccctcgacc aggacactgg tccgtagccg tttatctttg
     1381 ggatcgggag aggtacaaac ccggcaggtg caaccgatca tcggaacgcc ctgtgatgtt
     1441 ccggttccga gaaaggtgat acgcatggtt cgggtcagga agcgggttgt tccgaaggct
     1501 gaagtttcat ttgccgtgca tccgcaagca gtttgcggta gcgatcatcc agcagggact
     1561 catccaactc gagggattga atcgcttcga tcaggtagct gaccttggct tcgaccggag
     1621 agaacttatt gagaacgatg atcttcgaag cttcgacgat acaactgcct gacttgaaat
     1681 tccctttttc gtgacgaacc ttataaccgc ctaaaaccag cagttcttcg agtttgtcca
     1741 gggtggcctg tgtgagtttc agcataggat acaaatatac aaacacgatc gcggccaaca
     1801 ccccggactg ccaattttaa cccccaattc cattaaaatt cgtttctttg cctgccaaat
     1861 tccgaaggat aaacgctttt aacgtgatct tagccgtcac taaagaaacc aaggatcgtg
     1921 aacgccgcgt ggcgctgacc cctgaggttg tccagacact catcaaagcc ggatacgaag
     1981 tccgggtcca gtcaggcgcc ggcctgaatg cctactacga ggactccgct tatgaaaaag
     2041 ccggggctca ggtacaggca gaccgccaac aggtgcttca atcggccgat gtgctcttaa
     2101 acgtcaaccc gccttcaccg gacgatattg ccaagctgaa gaagggcgct gtcgtgatca
     2161 gtttccttta cgctgcgatc aatcccgcgg tagtcgaggc ctgtgtcagg cagggcgtga
     2221 gcgcgttctc catcgatgcg atcccccgta tttcccgtgc acagaagatg gatgctttgt
     2281 cgtctcaggc caacctggcc ggctataagg cggttttgtt gggtgccaca gcccttggga
     2341 agatctttcc cttgttgatg acggctgcag gtacgcttaa gccctccaaa gttgtcatta
     2401 tgggcgccgg tgtcgccggc ctgcaggcca ttgctacggc aaaacgcctg ggagcgatcg
     2461 ttgaagtatc cgacatccga ccggagacca aagaacaggt agaatcactc ggaggcaagt
     2521 tcatc
//
LOCUS       DAOTON010000404         2523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_32177_length_2523_cov_3.549028, whole genome shotgun sequence.
ACCESSION   DAOTON010000404 DAOTON010000000
VERSION     DAOTON010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32177_length_2523_cov_3.549028"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..2163
                     /locus_tag="P5292_08360"
     CDS             73..2163
                     /locus_tag="P5292_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39177.1"
                     /translation="MPVELITEHSPLWLVACLLLGLLYAFVLYRYPAVESLGKVLHRL
                     LFLFRALVVSFLAFFLLSPLLRTLSRETEKPLLLLAADNSQSLLLNKDSAYYRSDYST
                     DLHRFAAKLDDQFEVRWLRFGEEVRESDTLDLKDRQTDLSSLFSESAIRYANRNVGAI
                     VVASDGIINKGINPAYVSTGIQAPVYSILLGDTIVRKDLFIARVNHNKTAYLGNTFPI
                     NVVVEARQLSGQQMTLTVKEDSIVRFSRNFPITGNRFRQEIPVLLEALKKGLHHYKIE
                     LNQLQGEVTYTNNERDVYIEVLEQKQQILILAAAPHPDLGAFREVLERTRNYEVKTRR
                     FEKFDGNLSGLNLLVLHNLPSRNQNPEALLKKAADAGVPVLFILGTSLNVNAFNALQS
                     GLVIQGGSTDRTNAAGPVLDNNFSLFSLSDVLRGSIASFPPLLAPFGKYKPSGTPYVL
                     LNQQIGAVTSAEPLLFFTENGRSRRGFLCGEGFWRWPIADYSQNGSWDASQELLLKTV
                     QFLSASGNRSNFRVLARNSYAENEAIILDAEVYDQSYSLVNTPDVSLMLLRPDGTRFP
                     FTFSKSGNAYSLNAGFLAPGEYRYDANVLVGDKRYSATGRFSVTPIQAEREETVADHA
                     LMFNLAGKNGGAAFSRDQLDMLADTLLKREDIRTVSYSRIRLEDLIQVKWIFFLLMGL
                     LAAEWFIRKRSGLY"
     gene            complement(2157..>2523)
                     /locus_tag="P5292_08365"
     CDS             complement(2157..>2523)
                     /locus_tag="P5292_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS39178.1"
                     /translation="FLVPIGNERFRAGATYSWHELDQIPTEQAKKDLIDKLESVLRLP
                     FELIDHQAGIRPAVKGRRPLIGRHPKHANVFLLNGFGSKGVLMGPWLTENFADWLSGK
                     GTLHPETDIARYYIFVGDQ"
BASE COUNT          564 a          678 c          658 g          623 t
ORIGIN      
        1 ggggtggctg ctttcctttt gtatgggcag caaaaaagcg atgtcggccc ttaaatcgta
       61 tctttgaacc ctatgccggt agagctgatc acggaacact ccccgctttg gctagttgcc
      121 tgcctgctgc ttggactgct atacgccttc gtgctctacc gatacccggc tgtggaatcg
      181 ctgggtaagg tgttgcatcg gctgttgttc ctgttcaggg cgttggttgt ttcattcctc
      241 gcgttttttc tgctctcccc tttattgcgt accctttcaa gggaaacgga gaaaccgctt
      301 ctattgcttg ccgcagacaa tagccagagt cttttactca acaaggactc cgcttattat
      361 cgttccgact attcaaccga cctgcatcgg tttgccgcga agctcgatga tcagtttgaa
      421 gtccgctggc tgcgcttcgg agaagaagtc cgggaatccg ataccctcga cctgaaagac
      481 cggcaaacag acctttcgag tttgttctcg gagtccgcaa tccgctatgc aaaccggaat
      541 gtaggcgcta tcgtagtggc gagcgacgga atcatcaaca aaggcatcaa tcctgcctat
      601 gtttcgaccg ggatacaggc tcctgtctat tcgatcctgc tgggcgatac gattgtcagg
      661 aaggatctct tcattgcccg ggtcaatcac aacaagacgg cttatctcgg aaataccttt
      721 cccatcaatg tcgtggtgga agcccgacaa ttatccggtc agcaaatgac cttgacggtg
      781 aaagaggatt ccattgtccg gttcagccgc aacttcccga tcaccggtaa ccgtttccgc
      841 caggaaatcc cggtcttgct ggaagcgctc aagaaaggcc tgcaccacta caagatcgag
      901 ttgaatcagt tgcagggcga agtgacctat acgaataatg aacgggatgt ctacatcgaa
      961 gtgctggagc aaaaacaaca gattctgata cttgccgccg cgcctcatcc agacctcgga
     1021 gcctttcggg aggtactcga acgcacccgg aattatgagg tcaagacccg acgattcgag
     1081 aaattcgacg gcaacctgag cggattgaac ctgctcgtct tgcacaacct gccttcccgc
     1141 aaccagaacc cggaggccct tctcaaaaag gctgccgatg ccggtgtccc ggtcttgttt
     1201 atcctgggta catcgctcaa tgtaaacgca tttaatgcgc tccagtccgg tttggttatc
     1261 cagggtggta gcacggatcg gacgaatgcc gccggaccgg tactggacaa caacttttcg
     1321 ctcttcagtt tgtcggatgt actgcgcggt tcaatcgcat cctttccgcc actgctggct
     1381 ccttttggaa agtacaagcc atccggtact ccctatgtac tgctgaacca gcagatcggc
     1441 gccgtaacca gcgcggaacc gttgttgttc ttcacagaaa acggacgcag ccgtagagga
     1501 tttctatgcg gcgaaggctt ttggagatgg ccgattgccg attacagcca gaacggatcc
     1561 tgggatgcaa gtcaggagtt gttgctcaag acggtgcaat tcctgtcggc atccggaaac
     1621 aggagcaact tccgtgtact cgcacgaaac agctacgctg aaaacgaagc gatcattctg
     1681 gatgccgaag tgtatgacca gagttactcg ctggtgaata ccccggatgt ttcacttatg
     1741 ttgctgcgtc ctgacggaac caggtttcca ttcactttct ccaaatcggg caacgcctat
     1801 tcgctcaatg cgggattcct ggcgccaggg gagtaccggt atgatgcgaa tgtcctggtc
     1861 ggtgacaagc gctatagcgc caccgggcgc ttcagtgtta cgcccattca ggcggaacgg
     1921 gaggaaaccg tagccgacca tgcgctcatg ttcaatctgg ctggtaaaaa cggcggtgca
     1981 gcgttcagtc gcgatcaatt ggatatgcta gccgatacct tgttgaagcg ggaagacatt
     2041 cgcaccgtca gctattcccg tatccgactg gaagacctga tccaggtaaa gtggatcttc
     2101 ttcctgctca tggggctcct ggctgccgaa tggtttatcc gtaaacgttc ggggctttat
     2161 tgatcgccga cgaaaatata gtagcgggcg atgtccgtct ccggatgcag tgttcctttt
     2221 ccgcttaacc aatcagcaaa gttctctgtc agccagggtc ccatgaggac tcccttgctg
     2281 ccaaatccat tcaacaggaa aacattggca tgcttcggat gacggccgat cagcggacga
     2341 cgccctttta cagccgggcg tataccggcc tggtggtcta ttaactcaaa gggcaggcgt
     2401 aatacacttt ccagtttgtc tatcaggtct ttttttgcct gttcagtcgg gatttgatcc
     2461 agttcatgcc aggagtaagt tgctccggct cgaaagcgtt catttccgat cggcacgagg
     2521 aat
//
LOCUS       DAOTON010000405         2517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_32294_length_2517_cov_3.472380, whole genome shotgun sequence.
ACCESSION   DAOTON010000405 DAOTON010000000
VERSION     DAOTON010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32294_length_2517_cov_3.472380"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="P5292_08370"
     CDS             <1..802
                     /locus_tag="P5292_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRS39179.1"
                     /translation="LSGFFFKLSVVPFQFWTPDVYEGAPIPVTAYLSVVSKGAMVFVL
                     GSQLNTAFQSFSPVWYELITWGAILTMTVGNLMAIRQDQLKRFLAFSSMAQVGYLLIG
                     VSASSAAGDASVLYFLLVYLFSNLAAFGVLGLVSGSTGCERISDLRGFSRTHPFLAWS
                     LAIALFSLAGIPPTAGFFGKFFLLTAGAAKGNYLIITLASLNMIVSLYYYLRVVRAMF
                     MERTDRPLGAIASPGSSHLALAICLLMILLAGVASPVYNYFYHAANGI"
     gene            814..1179
                     /locus_tag="P5292_08375"
     CDS             814..1179
                     /locus_tag="P5292_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39180.1"
                     /translation="MAINQNHPFEELDGIRCAIVEKAVSPERLSFLRPLLEYNGYEVK
                     VVASPPPKAAAAPAEGDTPAQSVPETFTVGVTDVAFNVTNAIFGRLLATPEGRVVTMD
                     YWKQRTPVSDDSTPYFQGK"
     gene            complement(1187..1405)
                     /locus_tag="P5292_08380"
     CDS             complement(1187..1405)
                     /locus_tag="P5292_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39181.1"
                     /translation="MAGLLTGGLFVLCGTLLTLLEEHWTLDAHHILLAQTTRLHQLLI
                     NFLPPLLLGLGYYMGEKAEHCKKTGTQA"
     gene            complement(1461..1820)
                     /locus_tag="P5292_08385"
     CDS             complement(1461..1820)
                     /locus_tag="P5292_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS39182.1"
                     /translation="MTKSLLIVRESGMDDQLIHSMIDPAASVCHVHSCFGLEESLIFL
                     KSTSRERKQLPDLIVIDLDLPDRGGIELLKELQNFIRECNFMPRVIVIGSKLDEQDTE
                     AAMVYPCVRSVVRKPVH"
     gene            complement(1826..2242)
                     /locus_tag="P5292_08390"
     CDS             complement(1826..2242)
                     /locus_tag="P5292_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS39183.1"
                     /translation="MGYCKALIIDDSEIDRFITSKILEQCRFADQVVCAASGEEGFTI
                     LLETFLDKAEPPAFIFLDINMPGMDGFEFLEIYQSLPEELRSHIRLVVLSSSTNPRDI
                     ERAYDNPVVDQYLNKPLQQEDLIALAQPWARRAQAS"
     gene            complement(2276..>2517)
                     /locus_tag="P5292_08395"
     CDS             complement(2276..>2517)
                     /locus_tag="P5292_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS39184.1"
                     /translation="KVRTHQPSLVIMDIRMRPVNGVMALLTLRKQQPDLPVVIYTNYA
                     YDNFRTMCQRFGANEFIDKSGDVNLLLEAIRAHLN"
BASE COUNT          516 a          659 c          698 g          644 t
ORIGIN      
        1 cctttccggt ttcttcttta aactttcggt ggtgccgttc cagttttgga cgccggatgt
       61 ctatgaagga gcgccgattc cggtgacggc ctacctctcc gtggtatcga aaggtgcaat
      121 ggtcttcgtg ttgggtagtc aactgaatac cgcctttcag tcgttctcac cggtttggta
      181 cgagctgatc acctggggag cgatcctgac catgacggtc ggtaacctga tggccatccg
      241 acaggaccag ctcaagcgat ttcttgcttt ctcctcgatg gcgcaggttg gttatctgct
      301 gatcggtgtg agtgccagtt ccgctgccgg cgatgcttcc gttctttact tcttgctggt
      361 ctacctgttt tcaaatctcg ccgctttcgg agtgctggga ttggtttccg gttcgactgg
      421 ttgcgagcgg atctcggatc tgcgcggatt ttcccgtacg catcctttcc tggcctggtc
      481 gctggcgatc gcgttgtttt ccctggcagg tatcccgccc acggctggtt tctttggcaa
      541 gttctttctc ttgacggccg gtgccgccaa ggggaactac ctcatcatta ccctggcatc
      601 gcttaacatg atcgtgtcgc tctattatta cctgcgggtc gtacgcgcga tgttcatgga
      661 acgtactgat cggccgttgg gtgcaattgc ctctcccggc agttcccatt tggcattggc
      721 catctgtctg ctgatgattc tcctggctgg cgttgccagt ccggtttaca actacttcta
      781 tcacgccgcg aacggcatct gatccaacct gcgatggcga ttaaccagaa ccaccccttt
      841 gaagaactgg acggcatccg ttgcgcgatc gttgaaaagg cggtcagccc ggagcgtctg
      901 agctttctcc gaccgctcct ggagtacaat ggttacgaag tcaaagtggt ggccagtcca
      961 cccccgaagg cggctgctgc tccagccgaa ggtgacactc cggcgcaatc ggtacctgaa
     1021 acgtttaccg tgggcgttac ggacgtggcc ttcaacgtca ccaacgcgat ctttggacgc
     1081 ttattggcta cgccagaggg ccgggtggtc acgatggatt actggaagca acgtacgccg
     1141 gtgtccgacg acagcacccc gtattttcaa gggaagtgat accggcttag gcttgggtgc
     1201 cggtcttttt gcagtgttcc gctttttcac ccatgtagta accaagtccg agtaggagcg
     1261 ggggcaaaaa attgatcagc agttggtgta atcgggttgt ctgggctaag aggatgtgat
     1321 gcgcatccag ggtccagtgt tcttccaaca gcgtcagcaa ggtgccgcag agaacaaaga
     1381 gaccgcctgt aagaaggccg gccattcccc atcgcattga agtagacaag ttcatagtga
     1441 tccgtactgg ttcggaacgc tcaatgaacc ggtttacgga ctaccgaacg tacacatggg
     1501 tagaccatgg cggcttcggt atcctgttca tcgagtttgg aaccgataac gatgacccgg
     1561 ggcatgaaat tgcattcccg gatgaaattt tgaagctcct tcaggagttc gatcccgccc
     1621 cgatcgggca ggtccaggtc gatcacgatg aggtccggaa gctgttttcg ctcccgggaa
     1681 gttgatttca ggaaaatcag ggactcttcg agtccaaagc aggaatgaac gtgacatacg
     1741 ctagcagcag gatcgatcat ggaatggatg agttgatcgt ccattccgct ttcgcggacg
     1801 atgagcagcg atttggtcat ttccttcagg acgcttgcgc ccgccgggcc catggttgtg
     1861 ccaatgcaat caaatcctct tgctgcaggg gcttgttcag gtattgatcg accaccggat
     1921 tatcatatgc tcgctctata tccctgggat tcgtagatga ggaaagtacc accagccgta
     1981 tatgcgagcg aagttcttcc ggcagcgact ggtaaatctc caagaactcg aatccatcca
     2041 tgccgggcat attgatgtcc aggaaaatga acgccggtgg ttcagccttg tccagaaatg
     2101 tttccagcaa aatggtaaat ccctcttcgc ccgatgccgc gcaaactacc tggtctgcaa
     2161 accgacattg ctccaagatc ttggaggtga tgaatcggtc gatctcggaa tcgtcgatga
     2221 tgagcgcttt acaatagccc attggttgta gttgggtgag aagtttataa aatcgttagt
     2281 tcagatgggc gcggatcgct tccagcagta agttgacatc tccgctttta tcgatgaatt
     2341 cgttcgctcc gaaacgctga cacatcgtcc gaaagttgtc gtaggcgtaa ttcgtataga
     2401 tcactaccgg cagatcgggc tgctgctttc gcagggtcag caacgccatt acgccattga
     2461 cgggacgcat gcggatatcc atgatcacga gcgaaggctg gtgggtgcgg acctttg
//
LOCUS       DAOTON010000406         2508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_32457_length_2508_cov_3.056665, whole genome shotgun sequence.
ACCESSION   DAOTON010000406 DAOTON010000000
VERSION     DAOTON010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32457_length_2508_cov_3.056665"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2508)
                     /locus_tag="P5292_08400"
     CDS             complement(<1..>2508)
                     /locus_tag="P5292_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39185.1"
                     /translation="MSPRGGANGTGCIYKSSADGSNISVQYSFPVTGFWGANPRYCSP
                     IQASNGLLYGVMSGGGLHNAGVLYSYNTTTGTFTSLYSFKGLSDGGVPMGDLMQASDG
                     NLYGMTSDGGNAIDNGVLFRYEISSGTYTVLHTFSSATGTNPFGGLVQASNGLLYGMT
                     NLGGNFSQGVIFSYDIANDTLIKILNLDATSGGKPYAGFIKAANGKLYATTTQGGVNN
                     LGTLIQFDPATNVLTKRYDFTTANGYFSYGKLYQHSSGLFYGLTRLGGTNSDGVLFRF
                     DDATATYSVRFTFNNSDAAVGGYPYSNLALGTDGKLYGTTEQTGSGSANGTIFNFDIS
                     TSAYTRVRTFSGISSGTGAEPRSGLWAASNGKIYGTCSTGGSGDHGTFWNYEPSTTIF
                     TYHSLRFLQYGARPNGQLMKAANGLLYGMTSEGGSNRSGVIFSFNPSSSTYTVLHDFA
                     LGTKANGGLLQASNGILYGLTEAGGSSGLGSLFSYNISTNTHTVLVNLSNALGALPAG
                     NLIQASDGKLYGMTAYGGANSSGVIFSYNISTSTYSKLADFNATTSGQRPYGSLVQAN
                     NGSLYGMNSTGSGTYSDGALFKFNPVTNVLTPLLAFSGTNGSGCRGSMIKAPDGMLYG
                     MTAYAGTLGGGVIFKVDPTTDAYTKLYDMNSTQTGFSGFGDLLLASDNSLYGVTYLGS
                     PGGFGSMFRYNLSTSSFTKLRDWSAPDPASHVYAKFLEELITITTSSVSSSVCSGATI
                     LVPYTVSGMVDTSNVFTAELSNASGSFTSPVAIGTKVSSNSGQDTIVAVIPSGTAPGN
                     AYRVRVVASSPSVTGSVTPTVMQIKRADQQLFLEQMGS"
BASE COUNT          602 a          657 c          680 g          569 t
ORIGIN      
        1 gagcccattt gctcgaggaa cagctgctga tcggctcgtt taatttgcat aacggtcgga
       61 gtcaccgatc cggttacgga tgggctggaa gcgactacgc gaacccgata agcgtttccc
      121 ggagctgttc ccgacggaat gaccgcgacg atggtatcct gtcctgaatt gctgctcacc
      181 ttggtgccga tcgccaccgg gctggtaaaa cttccggaag cattgctgag ttccgccgtg
      241 aacacgttgg atgtatccac cataccgctt acggtatagg gcaccaatat ggtggcgccc
      301 gaacaaacgg aggaggaaac ggaggaggtg gtgatcgtga tgagctcctc taagaactta
      361 gcatagacat gggatgccgg atccggcgca gaccaatcgc gcaatttggt aaaggacgac
      421 gtactcagat tgtaccggaa catactgccg aaaccacccg gactgcccag ataggtaact
      481 ccatacaagg aattgtcaga cgcaagcagc aggtcaccga atccggagaa gccggtttgc
      541 gtactgttca tgtcgtacaa cttggtataa gcatcggtcg tggggtcgac tttaaagatg
      601 acgccaccac ccagggttcc ggcatacgcg gtcattccat acagcattcc atccggcgcc
      661 ttgatcatgc ttccgcgaca gccacttcca ttcgttccgg aaaaagccag caagggcgtc
      721 aacacattgg taaccgggtt gaatttgaat aaggcgccat cgctataggt gccagaccct
      781 gtcgaattca taccgtacag ggagccgttg ttggcttgca ccaggctgcc atagggtcgt
      841 tgaccactgg tcgtggcatt gaaatccgcc agcttggaat aggtggaagt actgatgtta
      901 taactgaata ttacgccaga actgttggcg ccgccgtagg ccgtcatccc gtacaacttc
      961 ccatcactgg cctgtatcag gtttccggcc ggaagtgctc ccaaagcgtt actaaggttg
     1021 accaatacgg tatgggtatt ggtgctgatg ttgtagctga acaaactacc caagcctgaa
     1081 gatccacccg cttcggtcag accgtacagg atcccgttcg atgcctgcaa caatccgcca
     1141 ttcgcttttg tacctagggc aaaatcatgt agtacggtat aagtggagga ggatggatta
     1201 aaactgaaaa tcaccccgct acgattgctt ccaccttctg aagtcatccc ataaaggagg
     1261 ccgttggccg ctttcatcaa ttgtccgttc gggcgcgctc cgtactggag gaaacgcaga
     1321 gaatggtaag taaaaatcgt agtggaaggt tcgtaattcc agaaagtacc gtggtcgccg
     1381 gaacctccgg ttgaacaggt tccgtagatt ttgccattgg aagctgccca aagtccggat
     1441 cgcggctcgg cgcccgttcc ggaacttatg cctgaaaagg ttcgaacacg tgtataggcg
     1501 ctggtggaaa tatcaaagtt aaagatggtg ccattggcac ttccggatcc tgtctgctcg
     1561 gtcgtaccgt atagctttcc atctgttccc agcgccaggt tgctgtaggg atagccgccc
     1621 acagccgcat ccgaattatt gaaggtaaat cggaccgaat aggtagccgt cgcatcgtcg
     1681 aaccggaaga gtacaccgtc gctgttcgtg ccgcccaggc gtgtcaggcc gtaaaacaag
     1741 ccggaggaat gctggtagag cttaccgtag ctgaagtacc cgtttgctgt cgtgaagtca
     1801 tagcgcttgg tcagtacgtt tgttgcagga tcgaactgga tcagggtacc caggttgttc
     1861 acacctcctt gtgtcgtcgt agcatagagt ttgccattgg cggccttgat gaaaccggcg
     1921 tatggttttc caccgctggt tgcgtccagg ttcagtattt tgatcagtgt atcattcgca
     1981 atgtcgtatg agaaaatcac cccctgactg aagttaccac ccagattggt catgccgtag
     2041 agcaaaccgt tcgaggcctg gaccaaaccg ccgaaggggt tggtgcccgt agccgaacta
     2101 aacgtatgca agacggtgta ggtgccggat gaaatctcgt agcggaataa aacgccgtta
     2161 tcaatagcgt taccgccatc ggaagtcata ccgtacaggt tgccatcgct ggcctgcatc
     2221 aggtcgccca tgggcactcc gccgtccgac aatcccttga agctgtaaag cgaggtaaac
     2281 gtccctgtgg tcgtgttata actgtacaac acgcctgcgt tgtgtaatcc cccacccgac
     2341 atgacgccat acagtaagcc attggaggcc tgtattggag aacaatacct cggattggct
     2401 ccccaaaaac cggtgacggg aaaactgtac tgaacggaaa tattggagcc gtcagctgag
     2461 gatttgtaaa tacaacctgt accgttggcc ccgccccgcg ggctcatc
//
LOCUS       DAOTON010000407         2507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_32471_length_2507_cov_3.234095, whole genome shotgun sequence.
ACCESSION   DAOTON010000407 DAOTON010000000
VERSION     DAOTON010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32471_length_2507_cov_3.234095"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1065)
                     /locus_tag="P5292_08405"
     CDS             complement(25..1065)
                     /locus_tag="P5292_08405"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HRS39186.1"
                     /translation="MKKVLLLLSLLSAMIGRAHAQANNAILFTENGERFQVILNGVLQ
                     NASPETNVRLTSLVAPNYKCRIIFEDTKLGHLDFNMFFNDPGSEVTWNIKKNNKGEYV
                     TRWVSSVPLAQAPPAPANQTVVVYTTTPPPPVAVATPATTTTTTTVQQSQSSSSGNMG
                     MGDENISLNMGINVGGEGGSISINASGMGMDDGMSSSSSSSTTTTTTTTHSVTTTTTG
                     APGTVVVAPAPAPAPPPAQVVYVVGYNGPIGCPVPMAPNDFQSFKQSVQSKDFENTKL
                     TIAKQVIQNNCLTADQVRETMTLFDFENTKLDFAKFAYDRTYDRANYYKVNDAFEFES
                     SIEDLNRYIGGR"
     gene            1209..>2507
                     /locus_tag="P5292_08410"
     CDS             1209..>2507
                     /locus_tag="P5292_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRS39187.1"
                     /translation="MRSIFLIGAGRSSASLIEYLLRHADSDGYRLTIGDVSEASASEK
                     AGGHPAARCIRFDINDARLRDEEIKAADLVISLLPAHMHVPVAEACVRFGKHLVTASY
                     VSPEMLALDTAAKQAGVLLLNECGLDPGIDHMSAMEVIDRIRQQDGELLAFRSYTGGL
                     VAPESNDNPWGYKFSWNPRNVILAGQGTARFIEKGNYKYIPYQRLFRQIRKIVVEGAG
                     TFDGYANRDSLSYRKVYGIENIPTMIRGTLRQEGYCAAWDVFVRLGLTDDSYILEQSA
                     RMTYAELVEALLPDGFQGEDLPAQVAALCDLDPNGREMDMVRWTGIFEPVTIGLHNVT
                     PAQALQQLLERRWLLRPSDKDMIVMQHEFEYRADGKSWRQYASLIVKGKDNRFTAMAQ
                     TVGLPVAIAARRILAGTISMTGVQVPVVADIYRPILEELEA"
BASE COUNT          556 a          631 c          713 g          607 t
ORIGIN      
        1 taaggccggg gcttttcatg gggatcagcg accaccgata taccggttga ggtcttcgat
       61 cgaactctcg aattcgaatg cgtcgttcac cttgtagtag ttggcacggt cataggtccg
      121 gtcataagcg aacttggcaa aatcaagttt ggtattctcg aagtcgaaca aggtcatcgt
      181 ttcacggacc tggtcggcgg tgaggcaatt attttggatg acctgcttcg cgatcgttag
      241 cttggtgttt tcaaaatctt tcgactgaac actctgtttg aaggactgga aatcgttcgg
      301 agccatcggt accgggcaac cgatcggacc attataacct accacataaa cgacttgtgc
      361 agggggagga gccggtgcgg gagccggagc cactaccacc gtaccgggag cgccggtcgt
      421 ggtcgtggta accgagtgag tcgtcgtagt ggtggtggtc gtgctgctgc tggaagagct
      481 gctcatcccg tcatccattc ccataccgga ggcattgatc gagatggagc cgccttcgcc
      541 gcccacgttg atgcccatgt tcagactgat gttttcatca cccataccca tgttacccga
      601 agaagatgac tgggattgtt gaacggtcgt cgtggtagtg gtggttgccg gtgtggcaac
      661 agcaacagga ggtgggggtg tggtggtata aacgacaacc gtctgattcg ccggtgccgg
      721 cggagcctgt gcaagcggaa ccgaacttac ccaacgggta acgtattcgc ccttgttgtt
      781 cttcttgatg ttccaggtta cttcagaacc gggatcattg aaaaacatat taaagtcgag
      841 atgccccagt ttggtatcct cgaaaatgat gcggcatttg taattcggtg cgaccaggct
      901 ggtgagccga acattggttt ccggactggc attttgtaac acaccgttca ggataacctg
      961 gaatcgttcg ccgttttcgg tgaacagaat agcgttattc gcttgtgcgt gcgctcgacc
     1021 gatcatggcg gacaagagcg aaagaagtag taggactttt ttcatgggtt ggtttactgg
     1081 aaattgcgga tgcggcgaaa agccaacatc gtgccaaaca ggtgcaaatt taaataatcc
     1141 ggacttcaga atacgtgtta ccggctgatg cagtggaatc atttaccttc gcagaaattt
     1201 ccctgaaaat gcgatccatt tttctgatcg gtgcaggccg ctcttctgct tccctgatcg
     1261 aatacctgtt acgacatgcg gattcggacg gctatcggct tacgatcggg gatgtcagcg
     1321 aggcttcggc aagcgagaaa gcaggcggcc atcctgccgc ccggtgcatt cgattcgata
     1381 tcaacgatgc ccgcctacgg gatgaggaga tcaaagcggc cgatctggtc atttccttac
     1441 tgccggcaca catgcatgta cccgttgcag aagcctgcgt tcgttttggt aaacacctgg
     1501 tcacagcttc ttacgtgtct ccggaaatgc tagcgcttga taccgcagca aagcaggcag
     1561 gtgtgttgtt actgaacgag tgtggacttg atcccggtat tgaccatatg agcgccatgg
     1621 aggtcatcga ccggatccgc caacaggatg gtgaattgct ggcgttccgt tcctataccg
     1681 gagggctggt cgcgcccgag tcgaatgaca atccctgggg ctataagttt tcctggaatc
     1741 cgcgtaacgt aatactggcc gggcagggta ctgcccgatt catcgagaag ggtaattaca
     1801 agtacattcc ttatcaacgc ctttttcgcc agatcagaaa gatcgtagtg gaaggagccg
     1861 gaacattcga cggctatgcc aaccgcgatt ccttgtccta ccgaaaagtc tacggaatcg
     1921 agaacattcc gaccatgata cgcggtacgc tccggcagga aggttattgt gctgcctggg
     1981 atgtcttcgt acgtctggga ctgacggacg acagctatat cctcgagcaa tccgctcgca
     2041 tgacctatgc ggagctggtc gaagccttgc tcccggatgg ctttcagggc gaagacctcc
     2101 ctgcgcaagt tgccgctttg tgtgaccttg atccgaatgg ccgtgaaatg gacatggtcc
     2161 gctggaccgg catctttgaa cctgtcacca ttggtctcca caacgtgacg cctgcgcaag
     2221 cgctgcaaca actcctcgaa cgacgctggt tgttacgtcc gtcggataag gacatgattg
     2281 tcatgcagca tgagttcgag tatcgggcgg atggtaaatc ctggcgccag tatgcttcgc
     2341 tgatcgtgaa aggaaaggac aatcgcttta cggccatggc gcaaaccgtc ggactgccag
     2401 tcgccatcgc ggctcgtcgc atcctggccg gtactatctc gatgacaggc gttcaggttc
     2461 cggtcgttgc cgacatctat cgaccgatcc tggaggaact ggaagcg
//
LOCUS       DAOTON010000408         2495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_32676_length_2495_cov_2.356148, whole genome shotgun sequence.
ACCESSION   DAOTON010000408 DAOTON010000000
VERSION     DAOTON010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32676_length_2495_cov_2.356148"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..2449
                     /locus_tag="P5292_08415"
     CDS             368..2449
                     /locus_tag="P5292_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39188.1"
                     /translation="MMRSFILLAGLFLFTITQSIAQREANIWYFGANAGLDFNSGAPV
                     PLTNGQVNTAEGCSAISDSAGNLLFYTDGLWVYNRNHTSMPNGFGLLGSNTSNQSALI
                     IRQPGSFTTYYIFTVDGGNGPGGLCYSVVDMTAQGGLGDVTSKNVNLYTPTTEKLAAV
                     KHANGVDFWILSHESTTNNFQVYLLTAAGVNTTPVISSVGTVFTNFADFNGALKVSPS
                     HTKVGMAVWGSDYAELFDFNPGTGVLSNPITLPSITTWPYGLAFSPSGQRLYISTEGS
                     NELSQWDLSSNNAATINASRTLIANITTMGTLGALQLAPDGKIYLAGNSTADLCVINN
                     PDALGAACNLSINSVNLGGRFSYWGLPNFCTDYINFLTILANGNCFGDSTYFSLADTA
                     GLISVSWNFRDSITGANNFSTSFTPAHVFSLASNFTVMVTATYTTGTFTGYLTVSIVQ
                     CSSVVAGFQADTTLCVGSCTDFTNQSVNGLTYTWSFPGAATTTSTDTDPTGICYNLAG
                     TYDVSLIAHNGLQSDTLTLPGYIHVYAPPTAPILINQNDTLYLTQDYDSYQWYFMGAP
                     IVGATDSFFVVTQDGLYSVQLTDANGCIFFSRTSDIAVGTSEIEGIHGGLQVFANESE
                     LIIRYQTDRTLELTFSIYDAAGRLVHTSREQSQAGANTLRLQGLQLAKGLYVLHVSDG
                     KNILVQKFAER"
BASE COUNT          554 a          874 c          575 g          492 t
ORIGIN      
        1 cggctcccgg gcctgtaccc gtaagcacag caccatcagg acgattgaca gaaaacctgc
       61 cgacaaactc ttgcggaaca tagacccctg gcgccctcag gcgctagttc aagttaacga
      121 atcctcctag ggcagacaag tcctaaaagg ctcgaaatac gtccgcaaac aatgattaga
      181 agcggatcag gcaagcccga tcacccgcgg tcctgctctc ggctccctgc ggtcgcctcc
      241 atcagggcta acttgcagct tgccggctat cgattggctg ccattcgcca aataaaatgt
      301 aagcagactg gtttttccga aagggtcttg gaaaaaggca ttagctttat tgccctaacc
      361 ctccttcatg atgcgctcct tcattctcct cgcaggcttg tttctgttca ccatcacaca
      421 gagcatcgcc cagcgggaag ccaacatctg gtacttcggc gccaacgccg gactcgactt
      481 caactccggc gctccggtgc cgttaaccaa tggtcaagtc aatacggccg aaggctgctc
      541 cgccatcagc gattctgccg gcaacctgct gttctatacc gacggtctct gggtttataa
      601 ccgcaaccac acgtccatgc ccaacggctt cgggctcctg ggcagcaaca cctctaacca
      661 gagcgcgctc atcatccgcc agcccggctc cttcacgacc tactacatct tcaccgtcga
      721 cggcggtaac ggccccggcg gactctgcta ctcggtcgtc gacatgacgg cacagggcgg
      781 actcggtgat gtgacctcca aaaatgtaaa cctctacacg cccaccaccg aaaagctggc
      841 tgccgtcaag catgccaacg gggtcgactt ctggatcctc tcgcacgaat ccacgaccaa
      901 caacttccag gtgtatctgc tcaccgctgc cggcgtcaac actacgccgg tcatctcctc
      961 cgtaggaacc gtctttacca acttcgccga tttcaatgga gccctcaaag taagccccag
     1021 ccataccaaa gtcggcatgg ccgtctgggg cagcgactac gccgaacttt tcgatttcaa
     1081 tcccggcaca ggcgtcctct ccaacccgat cacgcttcct tccatcacga cctggccgta
     1141 tggcctggcg ttctccccga gcggacaacg cctctacatc agcaccgaag gctccaacga
     1201 actcagccag tgggacctca gctcgaacaa cgcggctacc atcaacgcca gccgcacgct
     1261 catcgcgaac atcaccacca tgggcacact cggagcgctg caactcgcac ccgacggcaa
     1321 gatctacctc gccggcaact ccaccgccga tctctgtgtc atcaacaacc ccgacgccct
     1381 cggtgccgct tgtaacctct ccattaattc cgtgaacctc ggaggccgtt tctcctactg
     1441 gggactgccc aacttctgca ccgactacat caacttcctc accatcctcg cgaacggcaa
     1501 ttgcttcggc gattccacct acttctccct cgccgatacg gccggtctca tcagcgtgtc
     1561 ctggaacttc cgggactcca tcaccggggc gaacaatttc agcaccagct tcaccccggc
     1621 gcacgtgttc agcctggctt ccaacttcac cgtcatggtg acggccacct ataccaccgg
     1681 cacctttacc ggttacctca cggtctccat cgtgcagtgc agctcggtcg tagccggctt
     1741 ccaggcagat acgaccctct gcgtcggctc ctgtaccgac ttcaccaacc aatccgtcaa
     1801 tggcctcacc tacacctgga gtttccccgg tgcagccacg acaaccagta ccgatactga
     1861 cccgaccggc atctgctaca acctcgcagg cacgtacgac gtcagcctca tcgcccacaa
     1921 cggcctgcag tccgatacgc tgaccctacc gggctacatc cacgtgtacg cgccgccgac
     1981 cgcgcctatc ctcatcaacc agaacgatac gctctacctc acacaggact acgacagcta
     2041 tcagtggtac ttcatgggcg cgccgatcgt cggggccacc gactcgttct tcgtcgtgac
     2101 gcaggacggc ctctacagtg tacaactgac cgacgcgaac ggctgtatct tcttcagccg
     2161 tacttccgac attgccgttg gcaccagcga gatcgaaggg atccacggag gtctgcaggt
     2221 attcgcgaac gaatcggaac taatcattcg ctaccagacc gatcgtaccc ttgaactgac
     2281 attttccatc tacgacgccg ccggccgact cgtccacacc tcgcgggaac aatcccaggc
     2341 aggcgccaat accctgcgtc tgcaaggact acaactggcg aaaggactct acgtcctgca
     2401 cgtcagcgac gggaaaaaca tactggtaca gaaattcgcg gagcgatgaa cgttgtaacg
     2461 tttgaacgtt acaacgttga acgttgaagg ttgca
//
LOCUS       DAOTON010000409         2492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_32725_length_2492_cov_2.134181, whole genome shotgun sequence.
ACCESSION   DAOTON010000409 DAOTON010000000
VERSION     DAOTON010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32725_length_2492_cov_2.134181"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1429
                     /locus_tag="P5292_08420"
     CDS             <1..1429
                     /locus_tag="P5292_08420"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HRS39189.1"
                     /translation="RYPLWALVFLAFFYASISFVNHSCFRTFGLDLGLYTNALYDYAH
                     FQFNDSSSFRSVPENLLGDHFDLLLPLFSPFSWIFGSWTLLLIQWAVLLFGACGIYRF
                     SILRTGRILDATLLMLVFGLHFGISGALSFDYHSNVVAAALLPWFFVGLQERRPLLLW
                     LTFTLILFARENMSLWMVFVATGACWYYRDPATRKALLLKSLIAGGWFMLVSAWWMPQ
                     LHEGGVLSQFRYSVLGKDIASALKTIVTDPALVIRTLFTDHLPVPGFKPGIKQEFWFI
                     FLLSGAWILFRVPGFIWMAVPILLQKLLNDQVQVWGVNDHYNAEFAPLLAIGGAIAAG
                     TVQQVRLRTVLLVGLLVLDIAATIRTMDRVQAFVRRENLRVYQAPHWKSVVDKKALSE
                     ALGLIPADVSVSAHTNLHPRVAWRDSALIFPKMAGCRYVLLLEQGNPFPFTQEDYRLQ
                     LDSLRHSDAYSLVFDQDGVILLRRK"
     gene            1552..2019
                     /locus_tag="P5292_08425"
     CDS             1552..2019
                     /locus_tag="P5292_08425"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRS39190.1"
                     /translation="MLQIGQKAPDFKLINTNKEEVTLANFKGKNLVVFFFPMAWTGVC
                     TKEMCSIQEDYNAYSGMNAETIGVSVDSFFALKRFGEDNKITFPLLSDFNKIMIRDYD
                     MILPDFAFGYKNVAKRATVVIDKEGIVRYIEVLPNPGEMPNMDAIKQAVQQLG"
     gene            complement(2070..>2492)
                     /locus_tag="P5292_08430"
     CDS             complement(2070..>2492)
                     /locus_tag="P5292_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRS39191.1"
                     /translation="PLGVILGLCIHSFLEGMPLVQHFTDANTQRSLLAGIILHHIPVA
                     LALTSMLLASHISKPATFFYLLLFASMAPLGAGASLLLSEYTTGDLSLFFDRAMAVGI
                     GIFLHISTTILFESYEEHRFNLYKLGIMLLGAAIALMS"
BASE COUNT          531 a          648 c          691 g          622 t
ORIGIN      
        1 ccggtatccg ctgtgggcgt tggttttcct tgctttcttt tacgcctcga tcagtttcgt
       61 caaccacagt tgcttccgaa ccttcgggct cgaccttgga ttgtacacca atgcgctgta
      121 cgattacgcg cacttccaat tcaacgacag ttccagtttc cgttccgttc cggagaacct
      181 gcttggcgat catttcgatt tgctgttgcc gttgttctca ccgttctcct ggatcttcgg
      241 ttcctggact ttgctcctga tacaatgggc cgtgctgctt ttcggtgcct gcgggatcta
      301 ccggttttcc atcttgcgga ccgggagaat actggatgcc accctgctca tgttggtctt
      361 tggacttcac ttcggcatat cgggcgctct gagcttcgat tatcacagca acgtggtggc
      421 agccgcgctc ttgccctggt ttttcgtcgg actgcaggag cgtcgtccgc tgctgctatg
      481 gctcacgttc acgctgattc tattcgcgcg ggagaacatg tcgttgtgga tggtcttcgt
      541 tgcaaccggt gcctgctggt attatcgtga ccccgctaca cgaaaagcgc tgttgctgaa
      601 gtccctgatc gccggcggct ggtttatgct cgtaagcgcc tggtggatgc cgcagctgca
      661 cgaaggtggt gtcttgtcgc agtttcgcta ctcggtcttg ggaaaggata tagcaagcgc
      721 actcaagacg atcgtaacgg atccggccct ggtgatccgg accctcttca ccgaccatct
      781 tcctgttccg ggattcaaac ccgggatcaa acaggagttc tggttcattt tcctgttgtc
      841 gggagcctgg atcctgtttc gtgttcccgg ttttatctgg atggctgtgc cgatattatt
      901 acagaagtta ctgaacgacc aggtacaggt ctggggggtg aacgatcatt acaatgcgga
      961 gttcgcgcca ctcctggcaa tcggcggagc gatcgcggcc gggaccgttc aacaagtgcg
     1021 actccgtacc gttttactcg tcgggttgct ggtgctggat atcgctgcta cgatacgaac
     1081 catggaccgg gtgcaggcat tcgttcgcag ggagaatctg cgggtgtatc aggcgccgca
     1141 ttggaagtcg gtagttgata agaaggcctt gagcgaagcc ctcggactga tacctgccga
     1201 cgtatccgtt tccgcccaca ccaacctgca tccacgggtg gcctggcgcg acagcgcgtt
     1261 gatattcccg aagatggcgg gatgtcggta tgtgctcctg ctggagcaag gcaatccgtt
     1321 tccgtttaca caggaagatt atcgtcttca gctggacagc ctgcgccatt ccgatgccta
     1381 ttcgctcgta ttcgatcagg atggggtgat cttgctgcga cggaagtgat gtcgcaggat
     1441 ggtcattggg atcatcaaag cttgatccaa agtcgggagt gaatgaacat ttcgcagttg
     1501 agcttgtttc gtccattata gctaaattcg cagctgctaa aaacagaccc tatgcttcaa
     1561 atcggacaaa aagccccgga tttcaaactt atcaatacga ataaagaaga ggtcaccctg
     1621 gccaatttca aaggaaagaa cctggttgtt ttcttcttcc ccatggcctg gaccggcgtg
     1681 tgcaccaagg aaatgtgcag catccaggaa gattacaatg cctattccgg catgaatgcg
     1741 gaaacgatcg gcgtttcggt cgatagtttc ttcgcactca agcggtttgg tgaggacaac
     1801 aagatcactt tcccgttgct ttcggatttc aataaaatta tgatccgcga ctatgatatg
     1861 attcttcctg acttcgcgtt cggctataag aacgtagcca agcgtgccac cgtcgttatc
     1921 gataaggaag gaattgttcg ctacattgaa gtactgccca atcccggcga aatgccgaac
     1981 atggatgcca tcaaacaagc cgttcagcaa ctgggctgat ccaactgatc aaatcaggaa
     2041 gccgctccga ccagggcggc ttttttgttt cagctcatca gggcgatcgc ggcgcccagg
     2101 agcatgatgc ccagtttgta gaggttaaac cgatgttcct cgtaggactc gaaaaggatc
     2161 gtcgtagaga tatgcaaaaa gataccgatc cccaccgcca tggcccggtc gaagaacagc
     2221 gaaagatccc cggtggtgta ttcgctcagt aataaactgg cacccgcgcc cagcggcgcc
     2281 atggaagcga acagcaacag gtagaagaac gtagccggct tgctgatgtg ggatgcgagc
     2341 agcatgctgg tcagtgccag ggctaccggg atatgatgca ggatgatacc ggccagcagg
     2401 gagcgctggg tgttggcatc ggtgaaatgc tggaccagcg gcatgccttc caggaacgag
     2461 tgaatgcaca atccgaggat gacgcctaac gg
//
LOCUS       DAOTON010000410         2484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_32860_length_2484_cov_2.906958, whole genome shotgun sequence.
ACCESSION   DAOTON010000410 DAOTON010000000
VERSION     DAOTON010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32860_length_2484_cov_2.906958"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2484
                     /locus_tag="P5292_08435"
     CDS             <1..>2484
                     /locus_tag="P5292_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS39192.1"
                     /translation="KKAEEWRKLGFGTVLSTQRDGVVRGTAACVLLGDGEEHELILRD
                     RAAAAYSFDKGISTQDYPSSLMGSIALLRQTWYDARWYKEGGNKKEYNITLESFNRNL
                     ELPQWFEAGDKQNELRVARIGKEFGVNFLIKGSGDEYERAAELKAAGCRLIVPVAFPD
                     AYDLSDPYDALGVSLHQLRNWELAPGNAATLAQQGIEFALTSSDLKNRGDFWKNIRKA
                     IARGLSEEQALKALTATPASFLGLSDKVGALRKGMLANFLVTSKGLFEKDNAILENWV
                     KGKRYQFVDPQLSDLRGNYTVRIANGNSLRLKVGGEAAAPEWYLYDDTSAIKAAASRL
                     GQQVNFSFEAKKREPKGLYRFTGLAEKSAFSGNVLLPSGEWSAWTATLDSAFVPVAKT
                     DSAKKEAPVTATVRYPNMAYGWKELPKATTVLFRNATVWTNESDGILKNADVLISDGK
                     IRQVGSGIKAPAGAEVVDATGMHLTAGIIDEHSHIAISGSVNEATQSSSAEVRIGDVL
                     DADDVQIYRQLAGGVTASHLLHGSANPIGGQTQLIKLRWGRSPEELKFENWPGYIKFA
                     LGENVKQSHWGDKQVIRYPQTRMGVEQVYSDYFTRAREYDQAMNAWSKGDPKLRSGIP
                     PRRDLELDALAEILNGKRFITCHSYVQSEINMLMHIADSFGFKMNTFTHILEGYKVAD
                     KMKAHGVRGVSSFSDWWAYKYEVYEAIPYNGAIMQKLGLTVGFNSDDPEMARRLNQEA
                     AKAVLYGGVSEEEAWKFVTLNPAKMLRVDDRVGSIKVGKDADLVLWSQHPLSSYARPQ
                     KVWIDGREYFDQAADQAEQARIAQARAELL"
BASE COUNT          573 a          728 c          740 g          443 t
ORIGIN      
        1 caagaaagcc gaagagtggc gtaagctggg tttcggaacg gtactctcga cgcaacgcga
       61 cggcgtcgtt cgcggcacag cggcctgtgt cctgctcggc gatggcgaag agcatgagtt
      121 gatcctgcgc gaccgcgctg ccgcggccta ttcattcgac aaaggcatct cgacgcagga
      181 ttatccttcg tcgctgatgg gcagcatcgc cctgctgcgc cagacctggt acgatgcacg
      241 ctggtacaag gaaggcggca acaagaaaga atacaacatc acgctcgaat cgttcaaccg
      301 taacctcgag ctgccgcaat ggtttgaagc aggcgacaaa caaaacgaac tccgggtcgc
      361 acggatcggc aaagagttcg gggtgaattt tcttatcaaa ggaagcggag acgagtacga
      421 gcgtgcggcg gaactcaaag ccgccggttg ccgcctgatc gttcccgtag cattccccga
      481 tgcgtacgac ctctcggatc cgtatgatgc gctcggtgtt tcgttgcacc agttgcgcaa
      541 ctgggagttg gctcccggta atgctgccac gcttgctcag caaggcatcg agttcgcgtt
      601 gaccagcagc gacttgaaaa accgcggcga cttctggaag aacattcgga aagccatcgc
      661 gcgtggactg tccgaggagc aggcgttgaa agcactgacc gctacccccg cttcctttct
      721 gggcctgtcc gacaaggtcg gggcactgcg caagggcatg ctggccaatt tcctcgtgac
      781 gtcgaaggga ctgtttgaaa aggacaatgc gatcctggaa aactgggtga agggcaagcg
      841 ctatcagttc gttgacccgc aactatccga tctccgcggc aactatacgg tacgcattgc
      901 caacggcaac agcctgcgcc tgaaagtagg aggagaggcc gctgctccgg aatggtacct
      961 ctacgacgat acctcggcca tcaaggctgc cgcaagtcgc ctgggtcaac aggtaaactt
     1021 cagcttcgaa gcgaagaagc gtgaaccgaa aggattgtac cgcttcaccg gccttgcaga
     1081 gaaatccgcc ttctccggaa atgtcttgtt gccttccggt gagtggagcg cgtggactgc
     1141 aacgctggat tccgccttcg tccccgttgc gaaaaccgac agcgcgaaga aagaggcgcc
     1201 ggtgacggcc accgttcgct atccgaacat ggcttatggc tggaaggaac ttccgaaagc
     1261 cacgaccgtc ctgttccgca acgcgaccgt atggaccaac gaatccgacg gcatcctgaa
     1321 gaacgcggat gtcctgatca gcgatggcaa gatcaggcag gtcggcagcg gcatcaaagc
     1381 tccggcaggc gccgaggtcg ttgacgcgac cggcatgcac ctcactgccg gcatcatcga
     1441 tgagcactcg cacatcgcca tcagcggctc tgtgaacgaa gccacacagt cgtcttccgc
     1501 cgaagtgcgc atcggcgacg tattggacgc ggacgatgtc cagatctacc gccaactggc
     1561 aggcggcgta acggcttctc acctgttgca cggatcggcg aatccgatcg gcggacaaac
     1621 gcaactgatc aaactgcgtt ggggccgctc tcccgaagaa ctcaaattcg aaaactggcc
     1681 cggatacatc aagttcgcgc tgggcgagaa cgtgaagcaa agccattggg gcgacaaaca
     1741 agtcattcgt tacccgcaaa cacgcatggg cgtggagcag gtgtacagcg attatttcac
     1801 ccgcgcacgc gaatacgacc aggccatgaa cgcctggagc aagggtgatc cgaagctgcg
     1861 gagcggaatc ccgccccgcc gcgacctgga actggacgcg ctggccgaga tcctcaacgg
     1921 caagcgtttt attacctgtc attcctatgt gcagagcgag atcaacatgc tgatgcacat
     1981 agccgattcc ttcggtttca agatgaacac cttcacgcac atcctggaag gctacaaagt
     2041 cgccgacaag atgaaagcgc acggcgttcg cggcgtatcc tccttcagtg attggtgggc
     2101 ttacaagtac gaggtgtacg aagccatccc gtacaacggg gcgatcatgc aaaaactcgg
     2161 actgactgtc ggcttcaatt ccgacgatcc ggaaatggcc cgtcgcctga accaggaagc
     2221 tgcgaaagcg gtcctctacg gcggcgtgag cgaagaagaa gcctggaagt tcgtcaccct
     2281 caacccggcg aagatgctgc gcgtcgacga ccgcgtgggc agtatcaaag tcggcaagga
     2341 tgccgacctg gtgctgtgga gccagcaccc gctgtcgtcc tatgcgcggc cgcagaaggt
     2401 gtggatcgac ggccgcgagt acttcgacca ggccgccgac caggccgagc aggcgcgcat
     2461 cgcgcaggcg cgcgccgagt tgct
//
LOCUS       DAOTON010000411         2484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_32861_length_2484_cov_2.874846, whole genome shotgun sequence.
ACCESSION   DAOTON010000411 DAOTON010000000
VERSION     DAOTON010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32861_length_2484_cov_2.874846"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2484
                     /locus_tag="P5292_08440"
     CDS             <1..>2484
                     /locus_tag="P5292_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRS39193.1"
                     /translation="ATIINPVAPTVSLASSSMVSCAGGSNGSATISVTGGNAPYAYAW
                     TPNVSTTASAGNLAAGTYTVRVTDANNCEAYVSIDITEPSALQTTVSSSDVRCAGSAT
                     GQASVQVNGGAGGFSFAWSPGGAINDTLYNASAGSYTVTITDANGCTRSATTIVGSPS
                     PIGLSFNATPASCNGGSDGQIILTASGGTGTLHYSWAGSTDTGFVRQSLAAGVYACTI
                     TDDNGCTQTGSVQVTEPAKITLSTASTPSDCQNNNGSAQVSASGGTGSYSYSWFPSGG
                     NAASASGLASGDYIITVTDANGCIMADTVTVGSTGGLQLTLASASMVKCFGGSDGSAS
                     VNATSGTPPYQYAWSPGGGTTNTLNGLSAGNYSVQVTDVSGCTVTLPVSITQPDVLQA
                     ATSTTAVSCFGASTGAATVTVTGGTSPYTYLWNPGNATTTGISNRPAGNYTVTITDAN
                     ACTTTSAASILQPPVINLNMQSTPVSCNATNGTATVIPSGGSGAFTFSWFPSNDTTAT
                     ADSLAPGTYVVVVTDGNGCTRSGNVTVNALPVPHVQVDSSRSVTCNGDTNGAVFCSVS
                     GGAAPFNYQWTGTAGTGSSLTNIGAGIYQLLVIDANGCRDSVLAQVQEPSPLAILLTT
                     ENVLCAGAASGRIFAEVGGGVPPYSYNWTPGGQVNDTAVALTGGTYQVFVTDSNGCTT
                     QGSAAITEPAAIQAIVTGVDALCATNCDGHGEVTIAGGVEPYFVTWCNGDTTRQVNNL
                     CGGDCQVLITDGNGCGISQTISLQQPDTLQLTVAHTNPSCQGCADGTASVDVSGGLPP
                     YAYDWSPVTGTADSISALVAGWYVVCVTDS"
BASE COUNT          530 a          705 c          723 g          526 t
ORIGIN      
        1 gcgaccatta tcaatcccgt tgcgccgacg gtcagtttgg catccagttc catggtcagt
       61 tgtgccggag gctcgaatgg ttccgccacc atcagcgtga caggtggcaa cgccccttac
      121 gcgtatgcct ggacaccgaa cgtcagcacg accgcttcgg ccggcaatct cgctgcgggt
      181 acctacaccg tgcgtgtgac ggatgccaac aattgcgagg cctatgtctc catcgacatc
      241 acggaaccgt ctgcgcttca aacgaccgtc agttcaagcg atgtgcgctg tgccgggagc
      301 gcgacaggac aggcgagtgt ccaggtgaac ggtggcgccg gaggattttc gtttgcctgg
      361 tcacccggag gcgcgatcaa cgacaccctt tacaatgcgt cagccggttc gtacacggtc
      421 actatcaccg atgcgaacgg ttgtacccga agcgcaacga caatcgtcgg ttctccgtca
      481 ccgatcggac tatccttcaa tgcgaccccg gccagttgta acggaggcag tgacggccaa
      541 atcatcctga cggcatccgg cggaacaggg acactgcact acagttgggc tggatccacc
      601 gacacgggat tcgttcggca gtcacttgct gcaggggtgt acgcgtgtac gattaccgat
      661 gataatggct gtacgcaaac cggttccgtc caggtgacag aacctgccaa gatcaccctt
      721 tcaacggcgt cgactccctc ggattgccag aacaacaacg gatcggctca ggtatccgct
      781 tccggaggca ccggctccta ttcgtattca tggtttcctt ccggaggcaa tgccgcttcg
      841 gcaagcggac tcgcttcagg agattatatc attactgtaa cggatgccaa tggttgtatt
      901 atggcagata cggtaaccgt aggcagcacc ggtggtttgc aattgacact cgcttccgcc
      961 tcgatggtca agtgcttcgg tggtagcgat gggtccgcat ccgtgaacgc aacatcagga
     1021 acgccgccat atcagtatgc ctggtcaccg ggaggcggaa cgacgaatac cctgaatggg
     1081 ttgagtgcgg gcaactattc cgtgcaagtg accgatgtca gcggatgtac cgtaacgctt
     1141 ccggtttcga ttacccaacc cgatgtattg caggcagcga cgagcaccac ggcagtcagt
     1201 tgctttggag cctcaaccgg cgctgctacc gtgacagtga caggtggaac gtcgccttac
     1261 acttatctgt ggaacccggg aaatgcgaca actaccggta tcagcaaccg gccggccggt
     1321 aactacacgg ttacgattac ggatgcgaat gcttgtacga cgacctcagc agcctcgatc
     1381 ctccaaccgc cggtgatcaa ccttaatatg caatcgacac cggtaagctg caacgcgacg
     1441 aacggcactg caaccgtgat tccttccggt ggttcgggag ctttcacatt cagttggttc
     1501 ccgtcgaacg atacaaccgc aacagcagac agccttgcac cgggaactta tgtagtcgtg
     1561 gtaaccgatg gaaacggatg tacccgatcc gggaatgtaa ccgtgaatgc gttgccggtc
     1621 ccgcacgtgc aagtcgactc gagccgttcc gttacctgca atggtgacac caatggcgcg
     1681 gttttctgtt ccgtatccgg cggcgcggct ccattcaact atcaatggac gggcactgcc
     1741 ggcaccggat ccagcctgac gaacatcggt gcgggtatct accaattgct ggtcatcgac
     1801 gcgaacggtt gccgggattc cgtactggcc caagtgcagg aaccttcgcc gctggccata
     1861 ctgcttacga ccgaaaatgt gttgtgtgcg ggcgctgcaa gtggtcgcat cttcgcggaa
     1921 gttggaggcg gagtgccgcc gtattcgtac aactggacac cgggtggaca agtgaacgat
     1981 accgcggtcg cgttgacggg tggaacgtat caggtcttcg ttacggactc gaacgggtgt
     2041 acgacccagg gaagtgcggc gatcacggaa cccgcggcta ttcaggctat cgtcaccgga
     2101 gttgacgcgc tttgcgcaac gaattgtgac ggacatggtg aagtgaccat tgccggtggc
     2161 gttgaaccgt atttcgtcac ctggtgcaac ggagatacga cccggcaggt gaataatctg
     2221 tgcggaggcg actgccaggt cctcatcacc gatggcaatg gttgtggcat atcacaaacc
     2281 atcagcctgc agcaaccgga tacactgcaa ttgaccgttg ctcataccaa tccttcctgt
     2341 cagggttgtg ccgacggtac cgcatcggtt gatgtgagcg gtggattacc tccgtatgca
     2401 tacgactgga gcccggtcac cggtacggct gattcgatca gcgcgttggt tgccggctgg
     2461 tatgtggtct gtgtaacgga ttcg
//
LOCUS       DAOTON010000412         2480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_32936_length_2480_cov_3.356289, whole genome shotgun sequence.
ACCESSION   DAOTON010000412 DAOTON010000000
VERSION     DAOTON010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32936_length_2480_cov_3.356289"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="P5292_08445"
     CDS             <1..455
                     /locus_tag="P5292_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRS39194.1"
                     /translation="YCDIGIAVSAPKGLMVPVIRNAESRSLAEIESTILELAGRAREG
                     KLSLEEMTGGTFTITNGGVFGSMLSTPIINPPQSAILGMHNIVERPVAVQGKVEIRPV
                     MYVALSYDHRIIDGRESVGFLVKVKQQLEDPIRMLFGGGDPMRSLLGL"
     gene            469..1008
                     /gene="thpR"
                     /locus_tag="P5292_08450"
     CDS             469..1008
                     /gene="thpR"
                     /locus_tag="P5292_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRS39195.1"
                     /translation="MFVAVPLPAELELALSSFLDRIAGIAGVRPTLRPNLHLTAYFIG
                     SVQAELTPVILSNVERIALELQAFALSPRIFEFRGKATHPSMIWLAFEQSVDFLKLHD
                     RLRLALATFQNTGNSIQQPVPHITLARIRGGHDVRSEVVLPDQLPAALKVDRIGLWRT
                     HSTPSGVRYERLSESRLRE"
     gene            complement(1009..1596)
                     /locus_tag="P5292_08455"
     CDS             complement(1009..1596)
                     /locus_tag="P5292_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRS39196.1"
                     /translation="MIAPKKVWTVYAESTPNPATMKFVASQFLLLEGHVEYTDPAQAG
                     NCPLARRLFEFSGITGVFIMSNFVTITKREGIEWFEFMPILREFIKSYLGSGDPVFTG
                     PANQMADASIGRTTVGNELEDRIIATLDEYVKPAVEQDGGAIQFKSFEDGKVTVILQG
                     ACSGCPSSTMTLKSGIEQLLRTIIPEVKEVVAEAN"
     gene            1684..>2480
                     /locus_tag="P5292_08460"
     CDS             1684..>2480
                     /locus_tag="P5292_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRS39197.1"
                     /translation="MRKETDFLVLGSGIAGLTFALKAAEKGKVMIMTKARADESNTKY
                     AQGGIAGVMHAPDTFEKHIRDTLIAGAGLCREDVVRMVVTEGAERIREIIEWGTRFDR
                     NAQGDYDLAREGGHSEHRVLHFKDATGNEIERALLEQVRKHPNIEIHSHYFAVDLITQ
                     HHLGEEVRRSMPGVACYGAYVLNTQTREIETVLSRITLVATGGAGQVYKATTNPIIAT
                     GDGIAMVYRAKGKVKDMEFIQFHPTALYNPGEHPSFLISEAVRGHGAV"
BASE COUNT          532 a          675 c          722 g          551 t
ORIGIN      
        1 attactgcga tatcggtata gccgtcagcg cccccaaagg cttgatggtc cccgtgatcc
       61 ggaatgcgga atcacgctcg cttgccgaga tcgaaagtac catcctcgaa ctggccggcc
      121 gggcacgtga aggcaagtta tcactggaag agatgaccgg gggaacgttc acgatcacga
      181 atggcggagt cttcggctca atgctctcga caccgatcat caacccgccg caaagtgcca
      241 ttctgggcat gcataacatt gtggaacgac cggtggcggt tcagggcaag gtggagatcc
      301 gaccggtcat gtacgtggcg ctgtcgtatg atcaccgcat cattgacggg cgtgaatcgg
      361 ttgggtttct ggtgaaggtg aaacaacagc tggaagatcc gatccggatg ctcttcggcg
      421 gaggtgatcc gatgcgttcg ttgcttgggc tctgatcccc aacaccggtt gtttgtcgcg
      481 gttccgttac ctgcggaatt ggaacttgcc ttatcgtctt ttctggatcg cattgcaggc
      541 attgccggtg tccgcccgac acttcggccc aatcttcatc tgaccgcata ctttatcggt
      601 tctgttcaag cggaactgac tcccgtgatt ctttcgaacg tggaaaggat cgcgctcgaa
      661 ttgcaggctt tcgccttatc tccgcggatt ttcgaattca gagggaaggc gacacacccg
      721 tcgatgatat ggctggcgtt cgaacaatca gttgatttcc tgaagctgca cgaccggctg
      781 cgacttgctt tggcaacctt tcagaatacg gggaattcga ttcagcaacc cgtaccacat
      841 attacgctgg cgaggatacg gggcggtcat gatgttcggt cggaagtggt acttccggat
      901 caacttccgg cagcattaaa ggttgatcgg attggattgt ggaggacgca ttccacccct
      961 tccggagttc gttatgagcg gctcagcgaa tcccggctgc gggagtgatc agttggcttc
     1021 cgcgacgact tcctttactt cgggaataat ggtgcgcagc aattgctcga ttcccgattt
     1081 cagcgtcatg gtagaggagg ggcaacccga acatgcgcct tgaaggatca cggtgacttt
     1141 tccgtcttcg aacgatttga actggatcgc tccgccgtcc tgttcgacgg ccggcttaac
     1201 gtattcatcc agtgttgcga tgatacggtc ttccagttcg tttccgaccg tggtgcgtcc
     1261 gatcgaagca tcggccatct ggttagcggg tcccgtaaag accgggtctc ccgatcccag
     1321 gtagcttttg ataaactccc gcaggatcgg catgaattca aaccactcga ttccttcgcg
     1381 cttggtgatc gtcacgaagt tgctcatgat aaacaccccg gttattccgg aaaattcaaa
     1441 cagccggcgc gcgagcgggc agttgcctgc ctgtgccgga tcggtgtatt cgacatgacc
     1501 ttccagcagg agaaactggc tggcgacgaa tttcatcgtc gcagggttcg gggtcgattc
     1561 ggcgtaaacg gtccagactt tcttcggcgc tatcatgata gggcaaaggt aagaaccctt
     1621 accggaggag gaaagttcag gcaggccggg gcattttgta tttttatcgc ctgaaacggc
     1681 attatgcgca aggaaaccga tttcctcgtt ctgggttccg gcattgccgg gctcaccttc
     1741 gccctgaaag cggcggaaaa gggcaaggtc atgattatga ccaaggcgcg agccgatgaa
     1801 tccaacacca aatacgccca gggaggaatc gccggtgtca tgcatgcgcc ggatacattc
     1861 gaaaagcaca tccgggatac cctcatcgca ggtgccggac tgtgcaggga ggacgtggtc
     1921 cgcatggtgg tcaccgaagg cgccgagcgg atccgcgaga tcatcgagtg ggggacacgt
     1981 ttcgatcgta atgcgcaggg cgactacgac ctcgcgcggg aaggtggtca tagcgaacac
     2041 cgggtgctcc acttcaagga cgcgaccggc aacgagatcg aacgggcctt gctcgaacag
     2101 gttcgcaaac atccgaacat cgagatccac tcccattact tcgcggtcga cctgatcacc
     2161 cagcatcacc tgggcgaaga agtccgtcgc agtatgcccg gcgtagcctg ctatggcgct
     2221 tatgtgttga acacccagac gcgcgagatc gagaccgtac tctcgcgcat tacgttggtc
     2281 gctaccggag gcgccggaca ggtttacaag gcaacaacca accccatcat cgcgaccggc
     2341 gacggtatcg ccatggtgta tcgcgccaag gggaaggtga aggacatgga gttcatccag
     2401 tttcacccga cagccttgta caatcccggt gagcatccgt ctttcctgat atccgaagcg
     2461 gtacgggggc acggcgcggt
//
LOCUS       DAOTON010000413         2479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_32946_length_2479_cov_5.261964, whole genome shotgun sequence.
ACCESSION   DAOTON010000413 DAOTON010000000
VERSION     DAOTON010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32946_length_2479_cov_5.261964"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="P5292_08465"
     CDS             complement(<1..163)
                     /locus_tag="P5292_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS39198.1"
                     /translation="MERKILIVEDEPKVAGFIRSGLSESSLASDWAADGESGIAMLEA
                     NNYDLLILDL"
     gene            complement(479..1201)
                     /locus_tag="P5292_08470"
     CDS             complement(479..1201)
                     /locus_tag="P5292_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRS39199.1"
                     /translation="MTASSRAYLPPAGPVFDRTAFGFTLSIDRCYREIRYDGADSSLN
                     AVFNDRQKHEDPMMGYSVGLSLRKQRSLHWILEAGLRYTIRGYQYRSADKTFGPAIDT
                     NYVTVTNVTQALDEILIRHQYRYVEVPLRLHYQFTENRLRYHVAAGIALGLPLGVTVS
                     TRYKFSNGEREVKRNQLPDQDLKISITPILSAGMEFYYSHRLRFYLDPTYQFALNDAA
                     ATTIRERLNSAGLQFGVIYRYR"
     gene            1499..1831
                     /locus_tag="P5292_08475"
     CDS             1499..1831
                     /locus_tag="P5292_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39200.1"
                     /translation="MAKGKRGRPKGSKNKNTSANAAKIVVKKGKRGRPKGSKNKKTLL
                     AEGMPLAAIAPFKGTGKRGRPAKVAQGVIGNGIRIVDGMALINLTGSRVNRILVIGSD
                     AKTPVTHL"
     gene            complement(1901..>2479)
                     /locus_tag="P5292_08480"
     CDS             complement(1901..>2479)
                     /locus_tag="P5292_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRS39201.1"
                     /translation="FVERSIYEKFRDAFVDGVKKLQVGDPMLDESWIGAIVSKQHYDK
                     VLSYIELAKQEGGRVLCGGTALHPEGRCKNGWFIAPTVIEGLSYDCRTNQEEIFGPVV
                     TLQPFDTEEEVLQYANSTKYGLACSLWTSNLSRTHRMAAKIHSGIVWVNCWLLRDLRT
                     PFGGMKGSGVGREGGFEALRFFTEEKNVCIRL"
BASE COUNT          627 a          612 c          650 g          590 t
ORIGIN      
        1 tcaggtcgag gatcaacagg tcataattat tggcctccag catcgcaata cccgattccc
       61 catcggcagc ccaatcggat gcgagcgagc tttcgctcag cccggagcgg atgaagccgg
      121 ccactttagg ctcgtcttcg acgattagaa tttttcgttc catcaattaa gaaaattcga
      181 ccggtcttgt atccggcaag gtcatgtgac cgcgctattg tcaaaggtca ggaattccgg
      241 ctcctgaatt tcattaaaat gaagttaaac ctaagtgaat tcttgtatct gacttaccct
      301 gaatagcaga cgaatcgcga ttagattcgc caacaaaaca atcggaagga tatcggtcat
      361 tagccattcc cggtaaattg aaataatgca actcgaaaca cttagcctgg gtgagttttg
      421 tttcatgaaa accgttgaag tcttgtgaag ggaatcgcca ataaggaaaa cagctcgatc
      481 agcggtatcg gtagatcacc ccgaattgta agccggcact attcagccgt tcacgtatcg
      541 ttgtagctgc tgcgtcgttt agggcaaact gataggttgg atccagatag aatcgcaggc
      601 gatggctata atagaattcc atgcctgctg aaagtatggg tgtgatggag atcttgaggt
      661 cctgatcggg aagctggttg cgcttcacct cccgttcgcc attgctgaat ttgtaccggg
      721 tggaaaccgt tacgcccagg ggtaatccca aggcaatgcc ggcggcgacg tgatagcgca
      781 accggttttc ggtaaactgg taatggagcc gcaacggcac ttccacataa cggtattgat
      841 gccggataag gatttcgtcc agcgcttgtg tgacattggt cacggtcaca tagttggtgt
      901 cgatcgccgg gccaaaggtt ttgtcggcag accggtattg ataaccccgg atcgtgtaac
      961 gtaaaccggc ttccagaatc caatgaagtg agcgttgctt acgcagcgaa agaccgacgg
     1021 agtaacccat catcggatct tcgtgttttt ggcgatcgtt aaagaccgca ttcagactgg
     1081 aatccgctcc atcgtaccgt atttcgcggt agcaacggtc gatggataga gtgaatccaa
     1141 aggccgtacg gtcgaagacc ggtccagctg gcgggagata ggcccgggat gaggccgtca
     1201 tgaagcacaa gaaaaccatt gaaatcagcc ggctccgcat atgggtcaag gtagtattat
     1261 tcccggaatc cgtagtagga ttcacattgg attcattgta agggagccgg gtcgaatttt
     1321 ctgcattttc gattatttta gtgaataaat aactgcaaat acattgaatt atttttattc
     1381 aatcagttta tttggagtat acttgcaagg gagttagtga cgtgcattac tgcaactgtt
     1441 tcaaatccga tgataggaga ccatcggaat actatttaat caactaaata aattaattat
     1501 ggcaaaagga aaacgtggcc gcccgaaggg ttctaagaac aagaacacct ccgcaaatgc
     1561 tgccaaaatc gtcgttaaaa aaggcaaacg gggacgtccg aaaggatcga agaacaagaa
     1621 gaccctgttg gcggaaggca tgccgctcgc ggcaattgct ccgttcaaag gaaccggtaa
     1681 gcgtggtcgc ccggccaaag ttgcacaggg cgtcatcggc aacggtatcc gcatcgtcga
     1741 cggcatggcg ctcatcaacc tcacgggcag ccgtgtgaac cgtatcctcg tcatcggcag
     1801 cgatgcgaag acgccggtta cgcatctctg atcggttatg cacaataaaa aaggcgtcgt
     1861 gaaactcacg acgccttttt tatttaccgt agttgtctgc tcaaagccga atgcaaacat
     1921 tcttctcttc cgtaaaaaac cgcagcgctt cgaaaccgcc ttcgcgaccg acgccgctgc
     1981 ctttcatacc gccgaagggt gtgcgcaggt cgcgcagcag ccagcagttc acccagacga
     2041 tgccgctgtg gatcttcgcc gccatgcggt gcgtgcgact caggttggaa gtccagagtg
     2101 aacaggccag tccgtacttg gtgctgttcg cgtattgcag gacttcttct tccgtatcga
     2161 agggttgcag ggtaaccacc ggcccgaaga tctcttcctg gttggtgcgg cagtcgtagg
     2221 aaagcccttc gatcacggtc ggtgcgataa accaaccgtt cttgcagcga ccttccgggt
     2281 gcaaggcagt tccaccgcaa agaacacgac caccctcttg tttggcgagt tcgatgtagg
     2341 agaggacctt gtcgtagtgt tgtttggaca cgatcgcgcc gatccagctt tcatccagca
     2401 tcggatcgcc gacctgcagt ttcttcacgc cgtccacgaa cgcgtcgcgg aacttttcgt
     2461 agatgctgcg ttcaacgaa
//
LOCUS       DAOTON010000414         2471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33099_length_2471_cov_3.322434, whole genome shotgun sequence.
ACCESSION   DAOTON010000414 DAOTON010000000
VERSION     DAOTON010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33099_length_2471_cov_3.322434"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="P5292_08485"
     CDS             complement(<1..725)
                     /locus_tag="P5292_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRS39202.1"
                     /translation="MSVLVYVENLQGRFKKSAFELVSYANAIAGKLGMPLTALSIGEV
                     SAEELARLGKYGAAKVLSATDARLKQTNIQPYSTVIAEAAKSAGAKIVVVPASFSGKA
                     IAPRVAVKLGAGYADNAIDLPQMDGAFIVKKGAYSGKAYAFVEVVSDSKVISLVPNSY
                     KVVEAAAAGAVEVFTPAVPDSDFALQVKETVRATDKVSLPDAELVVSAGRGMKGPENW
                     GMIEQLADVLGAATACSKPVSDAG"
     gene            complement(761..1495)
                     /locus_tag="P5292_08490"
     CDS             complement(761..1495)
                     /locus_tag="P5292_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRS39203.1"
                     /translation="MKILVCISNVPDTTAKINFTPDNKEFVSSGVTFILNPYDEIALS
                     KAVDLAATGGTVTVINIGDGTTEPTIRKALAIGATDGVRINAAPRDSYFVAAQIADYV
                     KKNPFDMILCGRESSDNNGATVPAMIAEMLGIPCVPSAKSLQVEGGQVVMEREIEGGK
                     EIVACPTPLVVGASEGMAEWKIPNMRGIMSARTKPLQVVEPVEVPQLVRTVSYDKPAP
                     RSAVKMIAAEDAAKLFDILREEKKVI"
     gene            1661..1990
                     /locus_tag="P5292_08495"
     CDS             1661..1990
                     /locus_tag="P5292_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HRS39204.1"
                     /translation="MSTDRIPLLLSYLQDDPRDCFSRYALGLEYFKMNELDKAWGCFE
                     HLLKHAPDYLPAYYQAGQVLLRLNRPEEAAKALREGIVVASQQQDEHTRSELQGALLQ
                     LEDPDEG"
     gene            2061..>2471
                     /locus_tag="P5292_08500"
     CDS             2061..>2471
                     /locus_tag="P5292_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRS39205.1"
                     /translation="MRELQFREALREAMSEEMRRDERVFLMGEEVAQYNGAYKVSQGM
                     LDEFGERRVIDTPIAELGFAGIGVGAAMNGLRPIIEFMTFNFSLVAIDQVINSAAKMM
                     SMSGGQYPVPIVFRGPTASAGQLSSQHSQAFENWY"
BASE COUNT          553 a          680 c          686 g          552 t
ORIGIN      
        1 ccggcgtcgg aaaccggttt gctgcaagcg gtagctgcac ccaatacgtc agccaactgc
       61 tcgatcattc cccagttctc cggtcctttc attccccgtc cggcggagac gacgagttca
      121 gcgtcgggca gggaaacttt atcggtagcg cgaaccgttt ctttcacctg caaggcaaag
      181 tcgctgtcgg gaacagcagg cgtaaacact tccacagcac cggcagcagc ggcctccact
      241 accttatagg aattcggcac caggctgatg accttgctat cggagacaac ttccacgaag
      301 gcataggcct tgcccgagta agcacccttt tttacgatga acgcaccatc catttgcgga
      361 agatcgatcg cgttatcggc ataacctgca ccgagtttca ctgcaacccg tggagcgatg
      421 gccttaccgg aaaaactggc cggcaccact acgatcttcg ctccggccga tttcgcggct
      481 tcggcgatca ccgtgctata tggctggatg ttggtttgct tgaggcgggc gtcggtcgcg
      541 gatagtactt tcgccgcacc gtacttgccc aggcgggcaa gttcttccgc gctgacctcg
      601 ccaatggaca aggcagtaag cggcatgccg agtttgccgg caatggcgtt ggcataggag
      661 accagttcga aagcggattt cttgaaccgt ccctggagat tttcaacgta tacgagtact
      721 gacatgatga tctgctaaaa gtgattggcc gtgggccgga tcagatgact tttttctctt
      781 cgcgaaggat atcgaacaac ttcgccgcat cttcggcagc gatcattttc accgcgctgc
      841 gcggagcggg tttgtcgtag gagacggtcc ggacaagttg tggaacttcc accggctcga
      901 ctacctgcaa gggtttcgta cgcgcggaca tgatcccccg catgttcggg atcttccatt
      961 cggccatgcc ttcggaagcg ccgactacca gcggtgtggg gcaggcaacg atctctttac
     1021 cgccttcgat ctcccgctcc atcacgacct gtccgccttc cacttgcaga ctcttggcgg
     1081 agggcacgca gggaataccg agcatttcgg cgatcatcgc cggaacagtc gcgccattgt
     1141 tgtctgacga ctcgcggccg cacaggatca tatcgaaggg attctttttc acatagtcgg
     1201 cgatctgggc ggcaacaaag tagctgtcgc gtggagccgc gttgatccta actccgtcgg
     1261 tagcaccaat cgccagcgct ttccggatcg tcggttcggt ggtaccgtcg ccgatgttga
     1321 tcaccgtcac cgtaccaccg gtggcagcaa ggtccaccgc tttggataaa gcgatctcgt
     1381 cgtagggatt caggatgaag gtcacgccgg aagagacgaa ctctttgtta tccggagtga
     1441 aattgatctt cgccgtggta tccggcacat tggaaataca aaccaggatt ttcatgatga
     1501 cacgtctgtc gttggcggtc ggacgaaccg aacgtccggt tagttgaaca gcggatccgg
     1561 aataaggttc cggcaatact tcgtaatttc gtacgaaagg ccgtttttct tcgaccgctc
     1621 aagcgcgcga agataaggaa ttaccccttt tgaccaccgg atgtccacag accgaatccc
     1681 attgctattg agctacttac aagacgatcc ccgggattgt ttttcccgtt atgcattggg
     1741 acttgaatat ttcaaaatga acgagttaga caaagcctgg ggatgctttg aacacttgtt
     1801 gaagcatgca ccggattacc tgccggcgta ctaccaggca ggccaggtat tgctccggct
     1861 gaaccgaccg gaagaagcgg cgaaagcctt gcgggaaggg atcgtggttg ccagccaaca
     1921 acaggatgaa catacgcgat cggaattgca gggagcgtta cttcaactgg aagatcccga
     1981 cgaaggataa acccggaatt acctactttt gccgtccgcc gaagtgatcg tgacgaacga
     2041 ttgcgaagaa cgataacgct atgcgcgaat tacaattccg agaagccctg cgagaagcca
     2101 tgagcgaaga gatgcgccgc gatgagcgcg tcttcctcat gggtgaggaa gttgcccaat
     2161 acaacggggc atataaagta agtcagggta tgttggacga gttcggcgaa cggcgtgtga
     2221 tcgacacccc gatcgcggaa ctaggcttcg ccggaatcgg tgtcggagcc gcgatgaacg
     2281 gacttcgtcc catcatcgaa ttcatgacct ttaatttttc actggtagcc atcgaccagg
     2341 tcatcaattc cgccgccaag atgatgagca tgagtggcgg acagtaccct gtgccaatcg
     2401 tgtttcgggg cccgaccgct tctgccggac agctttcctc ccagcatagc caggcgtttg
     2461 agaactggta t
//
LOCUS       DAOTON010000415         2471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33100_length_2471_cov_2.982202, whole genome shotgun sequence.
ACCESSION   DAOTON010000415 DAOTON010000000
VERSION     DAOTON010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33100_length_2471_cov_2.982202"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="P5292_08505"
     CDS             <1..391
                     /locus_tag="P5292_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019949005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine dehydrogenase"
                     /protein_id="HRS39206.1"
                     /translation="DKVYEQAVDIYAPCALGATLNTENIGRLKCAVVAGAANNQLADE
                     NEHGRMLLEKGILYAPDFLINAGGLINVYSELKGYNREQAMEHTRKIYDTTLEIFAKA
                     EEESLTTIQAAKAIVEERLASARQVKA"
     gene            453..1385
                     /locus_tag="P5292_08510"
     CDS             453..1385
                     /locus_tag="P5292_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination protein NusB"
                     /protein_id="HRS39207.1"
                     /translation="MLSRRQLRVKVLQALYAYFQADKSDLAVAGRELFRSIEKVHELY
                     IYLLSLLRELADSDQADADDLHLKFFPKAEEVNAKHRLFNIRFIQAMVASRDFELFTR
                     RYHTSWQKDLDLVRKLFLEIKKSEEYRNFLLDDQANERDFLLLIMTRFLEPNETLEHH
                     VEEENIFWQEDFSFVCHIINRTIRTFYDAGRLELMPVYKDEADDKDFVRQLFEQTILN
                     NREYEAAVSKRTQNWDVERIAMMDILLLKMALAELVHFPGIPVKVSINEYIEISKDFS
                     TPKSKQFINGIIDKLAADYKADGKIVKTGRGLLE"
     gene            1427..1894
                     /locus_tag="P5292_08515"
     CDS             1427..1894
                     /locus_tag="P5292_08515"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRS39208.1"
                     /translation="MRKFTCTFAILLLIASSCKQADNNPVKPPDAISTEVVTNPASAS
                     GTNSKKGVPEFKFEQETWDFGQITEGEKISFAYQFTNVGNAELVIRAANGSCGCTVPE
                     FPKDPIPAGGKGIINVTFDSNGKTGMQHKTVTLISNTIPNTKVINVTGEVLPK"
     gene            1944..2261
                     /gene="yajC"
                     /locus_tag="P5292_08520"
     CDS             1944..2261
                     /gene="yajC"
                     /locus_tag="P5292_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRS39209.1"
                     /translation="MNLVLLQSQQNGALMQMLFLVGIIVVFYFFMIRPQMRKQKTEQE
                     FRNSLQKGAKVVTIGGIHGRIVEVADRTFMVEIDNNVRVRVERSAISAEATKALDQPA
                     SAK"
BASE COUNT          608 a          649 c          645 g          569 t
ORIGIN      
        1 ggataaggtg tacgagcagg ctgtcgatat ctatgctccc tgcgcgctcg gcgccaccct
       61 gaataccgag aacatcggac gattgaagtg tgccgtagtg gccggtgcgg ccaataacca
      121 gttggccgac gagaacgaac atggccggat gctgttggag aagggtatcc tgtatgcccc
      181 cgacttcctc atcaatgccg ggggactgat caacgtctat tcggaattga agggttacaa
      241 ccgtgaacaa gccatggagc atacccgtaa gatctacgat accacgttgg agatctttgc
      301 caaggcggag gaggaaagcc tcaccaccat ccaggccgcg aaggcgattg tcgaggagcg
      361 cctggcttcc gcccgccagg ttaaagcctg atcccagtag ttaccgagtg cctttgcgga
      421 tgcaggtccg ccagaacgtt ctttatcgat ccatgctcag ccgacgccaa ctccgagtca
      481 aagttttaca agccttgtat gcctacttcc aggccgataa gagtgacctg gcagttgcgg
      541 gccgtgagtt gttccgatcg atcgagaagg tacacgagtt gtatatctac ctgctttccc
      601 tgttgcgcga attggcggat tccgatcagg ccgatgccga cgacctccac ctgaagttct
      661 tcccgaaggc ggaggaggtg aatgccaagc accgcttgtt caacatccgg ttcattcagg
      721 ccatggtcgc ttccagggat tttgagctgt ttacccgtcg ctatcatact tcctggcaga
      781 aagacctgga ccttgtccgc aagcttttcc tggagatcaa aaagtcggag gagtaccgga
      841 actttctgct cgacgatcag gccaacgagc gggatttcct tctgctgatc atgacccgct
      901 ttctcgaacc gaacgagacc ctggagcatc atgtggagga ggagaatatc ttctggcagg
      961 aggacttcag tttcgtttgc catatcatca accggacgat ccgcaccttc tatgatgcag
     1021 ggcgcctgga gttgatgccc gtatacaaag acgaagcgga cgacaaggac ttcgtccgtc
     1081 agttgttcga acaaaccatc ctcaacaatc gcgagtatga ggccgcggtc agtaagcgca
     1141 cccaaaactg ggacgtggag cggatcgcca tgatggatat cctcttgttg aaaatggctc
     1201 tggcagagtt ggtgcatttt cccggaattc cggtgaaagt gtcgatcaac gaatacattg
     1261 agatctcgaa ggatttcagt acgccgaaaa gcaagcaatt catcaacggg atcattgaca
     1321 aactcgctgc cgattacaaa gcggatggaa aaatcgtgaa gaccggacgc gggttgctgg
     1381 agtaactgga acagccgcta tttttgttgt gtcaatcact gctaccatga gaaagttcac
     1441 ctgcaccttc gccatcctgc tgctgatcgc ctcgtcctgc aaacaggccg acaacaatcc
     1501 ggtaaagcca ccggatgcga tttccaccga agtcgttacg aatcccgctt cggcatccgg
     1561 gacgaattcc aagaaaggtg ttccggagtt caagttcgag caggaaacct gggacttcgg
     1621 tcagatcacc gaaggggaga agatctcgtt tgcctatcag tttaccaatg tcggaaatgc
     1681 cgaattggtc atccgggcgg cgaatggcag ttgtggttgt acggtacccg aattcccgaa
     1741 ggatccaatc ccggccggcg gcaaagggat catcaatgtg acctttgaca gtaacggaaa
     1801 gaccggcatg cagcataaga ccgtcacatt aatttcgaat accattccca ataccaaagt
     1861 gatcaacgta accggggaag tcctgcccaa gtgatcctag aaacattctg accgaaacaa
     1921 attccttaaa cgaaacttca ctcatgaacc tcgtactcct ccaatcccaa cagaacggcg
     1981 cgctgatgca gatgctgttc ctggttggta tcattgtcgt tttctatttc ttcatgatcc
     2041 gtccgcagat gcgtaagcag aagacggagc aggaattccg gaactctttg cagaaaggcg
     2101 ccaaagtcgt caccatcggg ggcattcatg gtcgtatcgt ggaagtagct gaccgcacct
     2161 ttatggtgga gatcgacaac aatgtccgcg tacgggtgga acgatccgcc atttccgccg
     2221 aggccacgaa ggcacttgat caaccggctt ctgccaagtg atttcaagac cgccgaaagg
     2281 cggttttttt gttattttac gggagtaatt tccattcgtg aaagacccac aagccggaac
     2341 ttctgttcga cctggcgcga acaagcggat atcgatcata ctggcatgtt tcggtatcgc
     2401 tactttgttt tggttgctga tcgccttgtc acacgattat gcagccacca tcacctttcc
     2461 tgtccgttat a
//
LOCUS       DAOTON010000416         2470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33106_length_2470_cov_3.834783, whole genome shotgun sequence.
ACCESSION   DAOTON010000416 DAOTON010000000
VERSION     DAOTON010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33106_length_2470_cov_3.834783"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="P5292_08525"
     CDS             complement(<1..324)
                     /locus_tag="P5292_08525"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRS39210.1"
                     /translation="MRFLKSRNQNPATDEELILAYRQDRDPARIGLLFDRYSSLLFGV
                     CMQYLRDEDESKDAVVHLFERFQHDLLKYEVRNFGSWAYRVCRSYCVRSLAKRKEVHD
                     EWALKN"
     gene            528..609
                     /locus_tag="P5292_08530"
     tRNA            528..609
                     /locus_tag="P5292_08530"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Leu,seq:tag)
     gene            646..2019
                     /gene="tig"
                     /locus_tag="P5292_08535"
     CDS             646..2019
                     /gene="tig"
                     /locus_tag="P5292_08535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRS39211.1"
                     /translation="MQISQEQQSELLTVLKIRLQPEDYQNRVKDILKRYQKSAQIPGF
                     RPGHVPAGLIKKQYGQPVLLDELNKLVSESISNYVFEQKLEVIGSPMPKNGLDQQTLE
                     DGNTFEFSYEVGLAPKFSVQLPSGKIPYYLVKVDEKMINDDADDLRRRYGKFSNPEKS
                     EENSILYGEFNELDEAGNVKEGGNKTTTTLSIERIPQVKDRKPFIGLAKGDTIDFHPM
                     TVIGNDTEVAAMLRLEKQSPAMQSNYRLTVMTISRIELADLNQEFFDKIYGEGNVTSE
                     EEFRAKIREGILAYFERESDRKMRKDLRNELLNQNNLPLPDDFLKRMLKANAEKPMAD
                     SEFDHQYYHLAEDLRWNLMVTKLATDQGFDVTEDELKELARGLVRQQFAQYGYYEIDD
                     AKLEEVSERYLNEDGNRERLDRSIRENKVFAHLKGVVKLDMIELPYQEYVARLQEKTQ
                     HELEHHH"
     gene            2245..>2470
                     /locus_tag="P5292_08540"
     CDS             2245..>2470
                     /locus_tag="P5292_08540"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133234.1"
                     /note="hydrolyzes proteins to small peptides; with the
                     ATPase subunits ClpA or ClpX, ClpP degrades specific
                     substrates; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRS39212.1"
                     /translation="MNYTNEFRKYAVKHQGISSLSVDSFVSAVRNDYISPTIIEERQL
                     NIATMDVFSRLMMDRIIFLGVGINDYVSNII"
BASE COUNT          655 a          624 c          646 g          545 t
ORIGIN      
        1 gtttttcaat gcccactcat cgtggacctc cttgcgtttg gccagtgacc ggacgcagta
       61 gctcctgcaa acgcggtagg cccaggaacc gaagttccgg acttcatact tcaacaagtc
      121 atgttggaac cgttcgaaca agtggacgac ggcatctttg ctttcatcct catccctcag
      181 gtattgcatg caaacgccga acagaagaga cgaatagcga tcgaacagca aaccaatacg
      241 ggccgggtca cgatcctgtc gataggccag gatgagttcc tcgtcggttg cgggattttg
      301 gtttctggac ttgaggaatc gcacagatcg ttgtcgcttg agcgggctcg ctgttttgcg
      361 gatgctttcc tttccagcta acgagcccgg gaagcgcaac atttcacaaa ctccgtacaa
      421 ttctgccgca agcccggcta tctcttcgag ttcagcaatt tagaaggaat cccattaggc
      481 ggaaagcggg gaaaaaccta cctttgcgct cccaaattcc gggaaaagcg gtcgtggcgt
      541 aattggtagc cgcgccagac ttaggatctg gtggagcaat ccgtgggggt tcgagtccct
      601 tcgaccgcac ataattatta ccggagtctt ctcaaataaa cgaacatgca gattagccaa
      661 gaacaacaga gcgaacttct tacggtattg aagatccgtt tgcaaccgga agactatcag
      721 aaccgggtca aggacatcct gaagcgttat cagaaatcgg cacagatacc cggttttcgt
      781 ccgggccatg ttccggcagg tctgatcaag aagcaatacg gacagccggt attgttggac
      841 gaactcaaca aactggtttc ggaatcgatc agcaattatg tattcgagca aaagttggag
      901 gtcatcggtt ccccgatgcc caaaaacgga ttggatcaac agacgctgga agacgggaac
      961 acgtttgagt tcagttatga agtaggtctt gcgccgaagt tcagtgtcca gttgccctcg
     1021 ggaaaaatcc cgtattacct ggtcaaggtc gacgagaaga tgatcaacga cgacgcggat
     1081 gacctgcgcc gccgttacgg aaagttctcg aatcccgaga agtccgaaga gaactcgatc
     1141 ctctacggcg agttcaacga actcgacgag gctggcaacg tgaaggaagg cggtaataag
     1201 accaccacca cgctcagcat tgagcgcatc ccgcaagtaa aagaccgtaa gcccttcatt
     1261 gggcttgcca aaggcgatac catcgatttc catccgatga cggtcatcgg aaacgatacc
     1321 gaagttgccg ccatgctgcg cctcgagaag cagtcgccgg caatgcaatc gaactaccga
     1381 ctgacggtca tgaccatttc acggatcgaa ttggctgatc ttaaccagga attcttcgac
     1441 aagatctacg gcgaaggaaa tgtgaccagt gaggaggaat tccgtgcgaa gatccgtgaa
     1501 ggcatcctcg cttactttga gcgggaaagc gaccggaaga tgcggaagga cctgcgcaat
     1561 gaattgctca accagaataa tcttccgtta cccgacgact tcctgaagcg tatgttgaag
     1621 gcgaatgcgg agaaaccgat ggcggattca gaattcgatc atcagtacta tcatttggcc
     1681 gaagacctcc gctggaacct gatggtgacc aaactggcaa cggatcaagg attcgatgtg
     1741 acggaagatg aattgaaaga actggcgcgg ggattggttc gtcaacagtt tgctcaatac
     1801 ggctactacg aaatcgatga tgcaaagctg gaagaagtat cggagcgtta cctgaacgaa
     1861 gacggaaacc gtgaacgact cgatcgcagt atccgtgaaa acaaggtctt cgcgcacctc
     1921 aaaggtgtcg tcaagctgga catgatcgaa ttgccttatc aggaatacgt agcccgcctg
     1981 caggagaaaa cccagcacga gttggagcac caccattgat ccggattttc cggcaggaat
     2041 tggaaaccag cggattggcc gaaacagcca gtaagttccg atcgggtttc caatttcttt
     2101 tttagggcca ggtaaccctg gatcggtttc cgctgttgaa aagttcggaa tcgtgtggca
     2161 tagcgccccg ctccgtccag taattttgga cttcccggca atatttccgt ggagttcccg
     2221 tctttcaaca gtaacgaaca catcatgaat tacaccaacg agttccgcaa gtatgcggtc
     2281 aagcaccagg gcatcagcag cctgagcgtc gacagttttg tttccgctgt gcgcaacgat
     2341 tatatctcac cgacgattat cgaagaacgt cagctcaaca tcgcgacgat ggacgtcttt
     2401 tcccgtctga tgatggatcg catcatcttc ctgggcgtcg ggatcaacga ttatgtttcg
     2461 aacatcatcc
//
LOCUS       DAOTON010000417         2468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33150_length_2468_cov_2.818483, whole genome shotgun sequence.
ACCESSION   DAOTON010000417 DAOTON010000000
VERSION     DAOTON010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33150_length_2468_cov_2.818483"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="P5292_08545"
     CDS             <1..935
                     /locus_tag="P5292_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HRS39213.1"
                     /translation="SGSLAGGPLGGMGDNVWNRDAVVAGRAGFDCAKGHPAMGNYHHH
                     QNPSAFNLDLSVISNVCDLYAADGLYVLDSTAHSPLIGFAYDGFPVYGAFGYANTNGT
                     GGITRMKSSYHLRNITARTHYANGTDVTDGPAVSATYPLGYFREDYEFVASTDPDRLD
                     EHNGRFCVTPEYPAGTYAYFCTVDENWNSAYPYIVGPTFYGTKVASRVNSITEPTTIY
                     TPSTTGLTNLVTDEASISIYPNPASDVVAVQLKSFLNADCPVVLFDAQGRIVERKILP
                     QGSTIAYFDTRRLYAGEYLVLIGNVSRRVVLVKD"
     gene            1167..1745
                     /locus_tag="P5292_08550"
     CDS             1167..1745
                     /locus_tag="P5292_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRS39214.1"
                     /translation="MQRSGESSIINISDLKDGGEDLIYQVYRRYRPDFIAWAKYQFDL
                     NEEDAKDIYQDVVLVMWKNIQSGRLTELTCEIKTYLFAIGKNLILNFRKKRDREVTLE
                     PVHLMNGIENTMEMNDKREYNKKLVEENLNRLPEHERKILELYYMQQKDMKQIAAEMG
                     YKNADVAKKKKYQVFKKLAALIKSNMKTFLLV"
     gene            1749..>2468
                     /locus_tag="P5292_08555"
     CDS             1749..>2468
                     /locus_tag="P5292_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39215.1"
                     /translation="MDEGLKHIELIEAYVEGRMSEADRAAFEVRIQTDADVFEEYELY
                     QKIRTSFSDIQEERMRDQFGRIDRDMDLEKTDGAGGSSGLRIWMAVAAVTVMAILIFW
                     KLFSGKFDSGQKIAASLPVEEGLPVLMSSVDRNKLFADAMTQFKLEDFRASSVLFSEL
                     SVKDPLNDTLLYFSGYSFLKLTEYPSAIDCFEKVVGQPGSIFFKKSQYCLSLAYWGAG
                     KKGKAEELSDLILQDPDHPYFQ"
BASE COUNT          600 a          584 c          711 g          573 t
ORIGIN      
        1 catccggatc gctcgccggc ggaccgttgg gcggcatggg cgacaacgtc tggaaccgcg
       61 acgcggtggt ggcggggcgc gcgggcttcg actgtgccaa ggggcatccg gcgatgggga
      121 actaccatca tcaccagaac ccgagcgcgt tcaacctcga tctgagcgtg atcagcaacg
      181 tctgtgacct gtacgcagcc gacggactgt atgtacttga cagcaccgcg cattctccgc
      241 tgatcggctt cgcctacgac gggtttccgg tgtacggcgc cttcggttac gccaacacca
      301 acggcacggg cggcattaca cggatgaagt caagttatca tttgcgcaac atcacggcgc
      361 gcacgcacta cgcgaatggc accgatgtca cggatggccc ggctgtcagc gcgacttatc
      421 cgctcggata cttccgcgaa gactacgagt tcgtcgcctc caccgacccg gaccggctcg
      481 acgagcacaa cggtcgcttc tgcgtgacgc cggagtatcc ggcggggacc tatgcatact
      541 tctgtaccgt ggacgagaac tggaattcgg cttacccgta catcgtgggg ccgacgtttt
      601 atggcaccaa ggtcgccagt cgggtcaaca gcatcaccga gccgaccacg atctacactc
      661 cttcgacgac cggactgacc aatttggtca ccgacgaagc gagcatcagc atctacccaa
      721 atcccgcgag cgatgtggtg gccgtgcagt tgaagtcgtt cctgaatgcc gactgtccgg
      781 tcgtgttgtt cgacgcccag ggtcgcatcg tggagcggaa gatcctgccg cagggcagta
      841 cgatcgccta tttcgatacg cgacgactat acgccggcga gtacctggtg ctgatcggga
      901 atgtgtcgcg gcgggtggtg ttggtgaagg attgagggcc aatactatat cttgttcgaa
      961 gccagctggt atcggaatgc acctgtagta ggcggggtga ctaggaatct ccgtcgggct
     1021 taagatattg gctgtcaact gcaggttgac ggcttttttg actcaatttt caggaaaatt
     1081 gcccggatat ttcagctgac ttgagtttgg agcctcagta agaatgcgta tttttaagcg
     1141 gcattgaact ttttgctctt gatcgtatgc agcggtcagg cgagtcatcg attataaata
     1201 tttcagactt aaaggacggc ggggaggacc tgatttatca ggtttatcgg cgttaccgtc
     1261 cggatttcat cgcgtgggcc aagtatcagt tcgatttgaa tgaagaagat gccaaggaca
     1321 tctatcagga tgtggtactg gtcatgtgga aaaacatcca gagcggcaga ttgaccgagc
     1381 tgacatgtga aatcaagacc tacttgttcg ccatcggcaa gaacctaatt ctgaacttta
     1441 ggaaaaagcg cgatcgggag gttacattgg aaccggttca tcttatgaat ggtatagaaa
     1501 ataccatgga gatgaatgat aaaagggaat acaataaaaa actggtcgaa gagaacctga
     1561 accgtttacc ggaacatgag cgaaagatcc tggagttgta ttacatgcag cagaaggata
     1621 tgaaacagat agcggctgag atggggtaca agaatgccga tgttgcaaag aagaagaaat
     1681 accaggtctt caagaagttg gcggccttga taaagagcaa catgaaaacg tttttattgg
     1741 tctgatccgt ggacgaaggg cttaaacata tcgagttgat cgaagcctat gtggagggcc
     1801 gcatgtctga agccgatcgt gcagctttcg aggtccggat tcagacggat gcggatgtgt
     1861 tcgaggagta tgaattgtat caaaagatac ggacctcgtt ctcggacatc caggaagagc
     1921 ggatgaggga tcagtttggc agaatcgacc gagacatgga cctcgagaag actgatgggg
     1981 ccggcggttc ttcagggctt cggatatgga tggccgttgc ggccgtcacc gtcatggcca
     2041 tccttatatt ctggaaactt ttcagcggaa agtttgacag cgggcagaag attgctgcct
     2101 cgttgcctgt tgaagaaggg ctgcctgtcc tgatgagttc ggtcgatcgt aacaaattgt
     2161 ttgctgacgc gatgacacaa ttcaagcttg aggatttcag ggcatcttct gttctgttca
     2221 gcgagctctc agtaaaagac cctttgaacg ataccctgct gtacttttcg ggatattcct
     2281 tcctcaagtt aacagagtat ccgagtgcaa tcgattgttt tgaaaaggtc gtcggacagc
     2341 ccggctcgat ctttttcaag aagagccaat attgtcttag ccttgcttat tggggagcag
     2401 ggaaaaaggg aaaagcggag gaattgtcgg atctgatcct acaggacccc gatcatccct
     2461 atttccaa
//
LOCUS       DAOTON010000418         2466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33182_length_2466_cov_3.174202, whole genome shotgun sequence.
ACCESSION   DAOTON010000418 DAOTON010000000
VERSION     DAOTON010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33182_length_2466_cov_3.174202"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1767
                     /locus_tag="P5292_08560"
     CDS             163..1767
                     /locus_tag="P5292_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRS39216.1"
                     /translation="MKSVSILRRFRSLLPVAFSLLALQPVMAQNGQSKLEYALQLIKF
                     WYVDTVNEKQLSDEAIRAMLKDLDPHSVYIPLEEMREMNEPLVGNFEGVGIQFNINND
                     TILITQTIPGGPSEKLGLRAGDRIVTINDSTVAGIKITNNDVLKKLRGPKGTKVKVTI
                     FRRGSADLLDFMITRDKIPLFSVDASYLVTPQIGYIKISRFADSTPSEFREALEKLKK
                     QGMTALILDLQGNGGGYLNKAVELSDEFLSEGKKIVFTRGRMTQSEDYMSSVMGGWEK
                     GKLAILIDESSASASEIVSGAVQDWDRGLIVGRRSFGKGLVQKSYGLPDGSMIRLTVA
                     HYYTPSGRCIQKPYEPGQEDEYDMDYVNRFKHGELFYADSIRFQDSTEYFTSAKRLVR
                     GGGGIMPDVFVPLDTSMSSTYYTDLLRKNILGDFTLNYVDRNRNKLLSAYPDMAVFKR
                     DFKPEGAFFEEFLEAASKGGVPMDSAGFRTSERLIRLQLKALVARNLWDIGAYFQVIN
                     DINVVLQRAIESINDNSFERLKISAR"
     gene            1876..>2466
                     /locus_tag="P5292_08565"
     CDS             1876..>2466
                     /locus_tag="P5292_08565"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRS39217.1"
                     /translation="MNWNLLNWPNTSDLVNDTTTRLPYYRTVVEYADPDILVTEENQT
                     SYSTTWFLNSVMNLRRPGVYRQGNYIQGPDSNNGIFYKDSLFRFVSNVPIETTLRDIN
                     VFTLVFKPTGDTLRVYAVHLKASTGVTNEAQRAQEVDSLRRRTDLLPAGTDFLVCGDF
                     NIYGDYEAAYQKLIVDRPVDDGNVVDVLNLTGIWNDP"
BASE COUNT          591 a          664 c          652 g          559 t
ORIGIN      
        1 ccctcatccg ggccatcgga taccgggaaa tgggtcggaa tgtggcgatt cgactccgcg
       61 ttccctttgg gatccacaac ggctcctgat caacccccga ctgcgtaaaa cctcccggga
      121 cttaggattt ccttaacgct cccgcaagta tctttgtgct acatgaaaag tgtatccatc
      181 ctccgacgct tccgctcgct attgccggtt gcgttttccc tccttgcgct ccagccggtc
      241 atggctcaga acggacaaag caaactggag tatgccttgc aattgatcaa gttctggtat
      301 gtcgatacgg tcaacgaaaa gcagttgtcc gacgaagcga tccgcgccat gctcaaggat
      361 ctcgatccgc attcggtcta tattccgctc gaagagatgc gggaaatgaa cgagccgctg
      421 gtcggtaact tcgaaggggt cggtatccag ttcaacatca acaacgacac gatcctgatc
      481 acccagacca ttccgggtgg tccatccgaa aaactcggac tccgggccgg cgaccgcatc
      541 gttacgatca atgactccac cgtcgctggc atcaagatca cgaacaacga tgtactcaag
      601 aaactgcggg gaccgaaagg aaccaaagtg aaagtgacca tcttccgtcg ggggagtgcg
      661 gacttgctcg acttcatgat cacccgcgac aagattccgc tcttcagtgt ggacgcatcg
      721 tatctcgtaa caccgcagat cggttatatc aagatatccc gttttgccga ctcaacaccg
      781 tccgagttcc gtgaagcgct cgagaaactc aagaagcagg gaatgactgc gctgattctc
      841 gatctgcagg ggaatggtgg cggctacctg aataaagcgg tcgagctttc ggatgaattc
      901 ctgtcggaag gaaaaaagat cgtattcacc cgtggccgca tgacacaatc ggaagattac
      961 atgtcttccg tgatgggcgg ttgggaaaag ggtaaactgg ctattctcat cgatgaatcc
     1021 agcgcttccg ccagtgagat cgtttcaggt gccgtgcagg attgggaccg cggactgatc
     1081 gtgggccgac gcagttttgg caaaggactc gtgcagaagt cgtacggcct gccggacggc
     1141 tccatgatcc gcctgaccgt cgcgcattac tatacgcctt caggtcgctg tatccagaag
     1201 ccctacgaac ccgggcagga ggacgaatac gatatggatt acgtcaatcg gttcaaacat
     1261 ggtgaactct tctatgctga cagtatccgt tttcaggatt ccaccgaata tttcaccagt
     1321 gccaaacgcc tcgtgagagg gggaggtggc atcatgccgg atgtatttgt cccactggac
     1381 accagcatga gtagcacgta ctataccgac ctgctgcgta agaatatcct gggtgatttc
     1441 acgctgaact atgtggatcg taaccggaat aaactgttgt ccgcttaccc ggacatggcg
     1501 gtgttcaaac gagatttcaa acccgaaggc gcgttcttcg aagagttcct ggaagctgcc
     1561 agcaagggcg gagtgcccat ggacagtgca ggatttcgta cctcggaacg cctgatccga
     1621 ctgcagttga aagcattggt cgcgcgcaat ctctgggata ttggcgccta cttccaggtg
     1681 atcaacgata tcaatgtcgt tctgcaacgg gcgatcgaat cgatcaacga taacagtttc
     1741 gagcgactca agatatcggc ccggtaatag aagatgatgg gtagatcgtt cgctgggaaa
     1801 ttcctgttgg caataggatg tgtcggactg tcggcgttaa atgtctttgc gcaaccagcc
     1861 ggttcgttta gcgtgatgaa ctggaacctg ctgaactggc ccaatacctc ggatctggtc
     1921 aacgatacaa caacgcgcct tccttattac cggacggtgg tcgagtatgc tgatcccgat
     1981 atcctcgtca ccgaggagaa tcagacctcg tacagcacaa cctggttcct gaactcggtc
     2041 atgaacctgc gcagaccggg tgtataccgc cagggtaatt acatccaggg ccccgacagc
     2101 aacaacggca ttttctacaa ggattcactt ttccgttttg tgagcaatgt gccgatcgaa
     2161 acgacattgc gcgacatcaa tgtcttcacc ttggtattta agccgaccgg cgacacgctt
     2221 cgggtgtatg ccgtacacct gaaggccagc acaggcgtca ccaacgaagc ccaacgcgca
     2281 caggaggtgg attcacttcg ccgccggacg gatctgctac cggcgggtac cgattttctc
     2341 gtttgtggcg acttcaatat ttacggagac tatgaagccg cctatcaaaa attgatcgta
     2401 gaccgccctg tcgatgacgg caatgttgtc gacgtcctga atcttaccgg catctggaat
     2461 gatccc
//
LOCUS       DAOTON010000419         2466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33185_length_2466_cov_2.888843, whole genome shotgun sequence.
ACCESSION   DAOTON010000419 DAOTON010000000
VERSION     DAOTON010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33185_length_2466_cov_2.888843"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="P5292_08570"
     CDS             complement(<1..1120)
                     /locus_tag="P5292_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39218.1"
                     /translation="MKRPSRQRYRRLFLIALAIPVICLLVFFSARSWLLEKALQKVQR
                     KLDERTGLKLDWRSDGFTGWRTVWITGVSLAPPAGDTLLTIDSVSVVPSIWGALTGTP
                     GISAIHVLKCTLQLVEQDGRSNYSALLKSGKDSTVTTQPSARGTSDYATLLHRLIDQA
                     FALAPQQARLDTIRFIWKRDTAFASLLVPEFRSDRDNLAGMLVDVRTNDRWTLRGSFS
                     QRKEVFDLTIAPAQQQKRLPLSILGWNLTAGFDTLHLALYESDFSKRTLKLKGHVSTE
                     GFFLHHARISDDTIRVEHARFDGLASVKGSTFELDSSSSAELNRIVVRLTAAFEKSQN
                     WKASLRLKTLPLEGSDFFRSLPEGIFETVRDIKADGTLS"
     gene            1360..>2466
                     /locus_tag="P5292_08575"
     CDS             1360..>2466
                     /locus_tag="P5292_08575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS39219.1"
                     /translation="MLRPENRSTLDELRQRAEKHVASNLNRFGLESLRKYDTWLDTLS
                     AQELGDSISILERQFERNSTSRHQELGSFQQQVDSLRLVKEQVTTSYHGLLRKSGIAF
                     AIWLLVVVIAIQIRRRLMGKASLRLAACKAQEEATRKAAQEGALLLQDVSSNVRRFEQ
                     SSLACRTLVQETTKADQDPANPWSKVSGFPDLLKSSTGLAQLIQRDYQVKEALNLLGQ
                     ADGDEKEKCDINRICETALDICTLGHSTEFEAPGLQVSRDLEKNLPQVSVNRRMVTGL
                     LIDILDNAFQAALDKQRQAIKGYQPKVSISTRVLPRFVQVRVKDNGIGISEEFQKTLF
                     EEFNTTRPLGAGTGLGLSESKRILTELHKGEIKIE"
BASE COUNT          594 a          677 c          661 g          534 t
ORIGIN      
        1 acgagagtgt cccgtccgcc ttgatatccc gcacggtctc gaagatgcct tccggaaggg
       61 aacggaagaa gtcactgcct tcgagcggaa gcgttttcaa acgcaggctc gctttccagt
      121 tctgcgattt ttcaaaagcg gcagttaggc gaacgacgat ccgattcaat tcagccgagg
      181 aagaagaatc caattcgaat gtagagccct tcaccgatgc cagtccgtcg aagcgggcgt
      241 gttcaacccg gatcgtatcg tcagagatcc tggcgtgatg gaggaaaaag ccttccgtcg
      301 aaacatgccc ctttaacttc agggtccgtt tactgaaatc ggattcatac aaggccaggt
      361 ggagcgtatc aaatcctgcg gtcaggttcc agccgaggat cgacagaggc agccgctttt
      421 gctgctgtgc cggagcgatc gtcaggtcaa acacttcttt tctctgcgag aaacttcccc
      481 gaagagtcca tcggtcgttg gtcctgacat ccacgagcat gcccgccagg ttatcccggt
      541 ccgaccggaa ctccggcacc agcagggatg caaaagccgt gtcgcgtttc caaatgaaac
      601 gaatcgtgtc gagccgggct tgttgcggcg ccaatgcaaa agcctgatcg atcaggcggt
      661 gcaacaaagt ggcatagtcg gatgttcctc gtgctgaagg ttgcgtggtg accgtactgt
      721 ctttccctga cttcaaaaga gcggaatagt tcgaacgccc gtcttgttcc accaattgca
      781 gggtacattt taagacgtgg atagcggaaa tacccggtgt gccggtcaag gctccccaaa
      841 tggatggcac gaccgacacc gaatcgatcg tcaataaagt atctccggcc ggaggcgcca
      901 gggacacacc cgtgatccac acggttctcc agcccgtaaa accatctgaa cgccagtcga
      961 gtttcagccc ggttcgttca tccagtttcc tttgcacctt ttggagggcc ttctccagca
     1021 accaggagcg cgcggagaag aagaccagga ggcagatgac tgggattgcc agggcaatca
     1081 ggaatagtcg tctgtaccgc tgtcgggaag gtcttttcat ggcatctacc ggcaacaggg
     1141 ggaggttacc ggtaacgcta cgaagatatt tgaacgagac aaattagctc tagccataca
     1201 cacaaatacg ttaataattt ttccaaacct tttccagaga ctttcagcag ctcggtaact
     1261 tgcttctcat acatgccggt tcatacttca ttcagatttt tactcctggc cagttgctgg
     1321 ctttgggcct ccgccattca ggctgaagga ccggccgccg tgcttcgccc ggagaatcgt
     1381 tctaccctgg atgagcttcg tcagcgtgcc gaaaagcatg tggcgtcgaa tctcaaccgc
     1441 ttcggcctgg aatcgcttcg gaagtatgac acctggctgg acacgctgag tgcgcaggaa
     1501 ctcggtgatt ccatctccat cctggaacga cagtttgagc gcaactcgac aagcagacac
     1561 caggaacttg gcagctttca acagcaggtc gattcacttc ggctcgtcaa agagcaggta
     1621 accacctcct atcacggctt gctgcggaaa tcaggtatcg ccttcgcgat ctggttgctg
     1681 gtcgttgtga tcgccatcca gattcgccgt cgtttaatgg gcaaggcgag tttgcggctg
     1741 gctgcttgca aagcccagga agaagctacc cgaaaagctg ctcaggaagg cgccctcctc
     1801 ttgcaggatg tcagtagcaa tgtccggcga tttgagcaat cctccctggc ttgccgaacg
     1861 cttgtacagg aaactaccaa agccgatcag gatcccgcca acccctggag caaagtgagc
     1921 ggtttccccg acttgcttaa atcctcaacc ggactcgccc aactcatcca acgtgattac
     1981 caggtgaagg aagcactgaa cctgctcgga caggccgatg gtgatgaaaa ggaaaagtgc
     2041 gacatcaacc gtatttgtga aacggccctg gatatctgta cacttggcca ttcgacggag
     2101 ttcgaagctc ccggtctgca ggtcagtcgc gaccttgaaa agaacctgcc tcaggtttcc
     2161 gtcaaccgga gaatggttac cggcttgttg atcgacatac tggataacgc gtttcaggcg
     2221 gcactggaca agcagcgcca ggcaatcaag gggtatcaac cgaaggtcag catcagcacc
     2281 cgcgtgcttc ctcgcttcgt gcaggttcgg gtgaaagata acggcatcgg gatcagtgag
     2341 gaatttcaga agaccctgtt cgaggaattc aataccaccc gaccactggg tgcaggtacc
     2401 ggattaggcc tctcggagag caaacggatc cttactgaac tccacaaagg cgaaatcaag
     2461 atcgaa
//
LOCUS       DAOTON010000420         2465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33198_length_2465_cov_3.661411, whole genome shotgun sequence.
ACCESSION   DAOTON010000420 DAOTON010000000
VERSION     DAOTON010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33198_length_2465_cov_3.661411"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..>1465)
                     /locus_tag="P5292_08580"
     CDS             complement(98..>1465)
                     /locus_tag="P5292_08580"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRS39220.1"
                     /translation="NTYNYFTNGLSTDASVDVRRTFKKPDQELSFAGQWTRNNRRTEY
                     ESSLDTLDLDYYKESSLNHGITDELTFQTDYTHPVTKAFTIETGAKAILRDVDSDYDY
                     RIFRFASDAYEYDPVRSNVYNNEQDIVAGYLQTSLNLKKFGIKAGLRYEHTTFKGYFD
                     KGDTRFSNEYENFVPSTTISYARQGKYSLKLSYTQRIQRPGLNFLNPFINATDPTNVT
                     YGNPDLDAEKSHAFELGLNLFKKFGSVNLSAYHRFTDNAIESIRFVDTNDVYITTYGN
                     IGKNRSTGMSLGLNVMWQMKIFLSSNFNLFYYQVESTGLSQNLNNDGVNWNVNLFGSY
                     KFSKRWGVMAFGNFNGPRYSVQGKSTSFWYYNLSARREFKNEKGGIGIGLDNFASWYM
                     HFRNNYSGEGFSYDNDTKVLFLGARISLDYRFGKMEYGGGQKKKKGIKNDDVKDDGGG
                     GMMGR"
     assembly_gap    1466..1475
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1476..2446)
                     /locus_tag="P5292_08585"
     CDS             complement(<1476..2446)
                     /locus_tag="P5292_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRS39221.1"
                     /translation="MACVTNKPVEFASVALYEKGSEKPIDGVITDEKGNFRIKNLKKG
                     TYRIGITFIGYVQKFVDSLVVTEKKPSVEVGTVRIGPSATMLKEANVEAEKQLIETRI
                     DKLVYNADKDITSKGGNATDILRKVPMLSVDLDGNVSLQGTQNVRILINNKPSSLMSA
                     NIADALKMIPADEIEKVEVITSPSAKYDAEGTGGIINIITKKKNIEGLSGSINAGAGT
                     RSSNLFGNLNYRSGRFGTGISGGGFGWIGIGDVSTTRVTQYSTLKQEGDNRNTGFGPS
                     LQWTGDVDLTSKLSLSTSLRVNNFQNRSKGTTDNFFAFTGDALRPSFT"
BASE COUNT          530 a          658 c          640 g          627 t
ORIGIN      
        1 attactagtt actagttact agttacacgc aatacaaagc tcacccgaca acaaaaaact
       61 cgaaacccga aactcgaaac ccgaaaccct gaacccacta ccgtcccatc atcccgccac
      121 cgccgtcgtc cttcacatcg tcgttcttga tgcctttctt ctttttctgg ccgcctccgt
      181 attccatctt accgaagcgg tagtcgagac tgatccgggc gccgaggaag agcaccttgg
      241 tgtcgttgtc gtagctgaag ccttccccac tgtagttgtt gcggaagtgc atgtaccagc
      301 tggcgaaatt atccagaccg ataccgatac cgcctttttc attcttgaat tcccggcgcg
      361 cgctgaggtt gtagtaccag aacgaagtcg atttcccctg aacgctgtaa cgcggtccgt
      421 tgaaattgcc gaaagccatc acgccccagc gcttgctgaa cttgtaagag ccaaaaaggt
      481 tcacgttcca gttgacaccg tcgttgttca ggttctgtga aagaccggtg ctctcgacct
      541 ggtagtagaa caggttgaag ttgctgctga ggaagatctt catctgccac atgacattca
      601 agcccaggct catgccggtc gagcgattct tgccaatgtt cccatacgtg gtaatgtaca
      661 cgtcgttggt gtcaacaaag cgaatgctct caatcgcgtt atcggtaaag cggtgataag
      721 ccgacaggtt gaccgagccg aacttcttga acaggttcag tccgagttcg aacgcatggc
      781 ttttctcggc gtcgagatcg ggattgccat acgtgacatt cgtcggatcc gtcgcattga
      841 tgaacggatt caggaaattc aatccgggac gttgaatgcg ttgcgtgtaa ctcagtttga
      901 gcgaatactt gccctgcctg gcgtaggaga tcgtcgtaga gggcacgaag ttctcgtatt
      961 cattactgaa acgggtatcg cctttatcaa aatacccttt gaaggtcgtg tgctcatagc
     1021 gcaaacccgc cttgatcccg aactttttga ggttaaggga agtctgcaga taacccgcta
     1081 cgatatcctg ctcgttatta taaacgttgc tcctcaccgg atcgtattca taggcatcgc
     1141 tggcgaagcg gaaaatgcgg taatcgtagt cgctgtcgac atcgcgcagg atcgccttgg
     1201 cgcccgtttc gatggtgaag gctttcgtca ccggatgggt gtaatccgtt tggaaggtca
     1261 actcatccgt aatgccgtga ttgaggctgg actccttgta atagtcgagg tcgagcgtgt
     1321 ccagactgga ttcgtactcg gtgcgccggt tgttccgggt ccattgaccg gcgaaggaaa
     1381 gttcctgatc cggcttcttg aaagtacgtc gcacgtccac actggcatcg gttgaaagac
     1441 cgttcgtgaa atagttgtac gtattnnnnn nnnnngtgaa cgacggacgc aaggcgtcgc
     1501 cggtgaaggc aaagaagtta tccgtagttc ccttcgagcg gttttgaaaa ttgttcaccc
     1561 gcaaagaggt gctcaggctg agcttactgg taaggtcgac atcgccggtc cattgcaggg
     1621 acggtccgaa tccggtgttc cggttatcgc cttcctgctt caaagtggag tattgggtga
     1681 cccgggtggt ggacacatcg ccgatgccga tccagccgaa gccgccgccg ctgataccgg
     1741 tcccaaaacg accgctgcgg taattcaggt tgccgaacag gttgctggaa cgcgtgcccg
     1801 ctccggcgtt gatggatccg ctcaggcctt cgatgttctt cttcttggta atgatgttga
     1861 tgatgccgcc ggtaccttcc gcatcgtact tcgccgacgg actggtgatg acttccacct
     1921 tctcgatctc atcggccggg atcatcttca acgcgtcagc gatattggcc gacatgagcg
     1981 agctgggttt gttgttgatc aggatacgga cattttgggt tccctgcagc gaaacattac
     2041 cgtccagatc gaccgatagc atcggaacct ttcgcaagat gtcggttgca ttgccgccct
     2101 tcgaggtgat atccttatcg gcgttgtaca cgagcttgtc gatccgcgtc tcgatcaact
     2161 gtttctccgc ttccacattg gcttctttca acatcgtagc ggaaggacca atgcgaacag
     2221 tacccacctc caccgatggt ttcttctccg tgacaaccaa ggaatcgacg aacttctgta
     2281 cgtaaccgat gaacgtaatg ccgatccggt aggtgccctt tttaagattc ttgatccgaa
     2341 aatttccttt ctcatcggtg atgacaccat cgatcggctt ctcgcttccc ttttcataga
     2401 gggctacact ggcgaattcg accggtttgt tggtgacgca cgccatgggc cagccggcgg
     2461 cgtgc
//
LOCUS       DAOTON010000421         2456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33379_length_2456_cov_3.149521, whole genome shotgun sequence.
ACCESSION   DAOTON010000421 DAOTON010000000
VERSION     DAOTON010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33379_length_2456_cov_3.149521"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="P5292_08590"
     CDS             complement(<1..1339)
                     /locus_tag="P5292_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRS39222.1"
                     /translation="MKSTGKSTVLPVLSADLPAALVVFLVALPLCLGIALGSQAPLFA
                     GLIGGIVGGIVIGTLSGSQLSVSGPAAGLTAIVAAAIGKMPVYEAFLLSVVIGGVIQL
                     LLGMLKAGVLGDYIPSSVIKGMLAAIGLILILKQLPHLVGYDADFEGDESFSHDGNNT
                     FSDLIQSVGYILPWAALIGIVSLLVQILWEKFLGNRGGLFRILPAPLVVVLIGTWLHL
                     WGTRQFPELALQSQHLVEIPVATSMSGFFSFFIFPDVQFLTNVDVWITGVTLAIVASL
                     ESLLCIEASDELDPYKRVTPTNRELKAQGVGNIVSGMLGGLPLTSVIVRTSANINAGA
                     RTKWAAIFHGVFLLLSVALIPELLNMIPKASLAAILIYTGYKLAKPSIFRSLYQNGWD
                     QFVPFLVTVLAILLTDLLVGILIGCCIGLFFVMRSNFRSAVFVVNDENHYLFRL"
     gene            complement(1501..>2456)
                     /locus_tag="P5292_08595"
     CDS             complement(1501..>2456)
                     /locus_tag="P5292_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRS39223.1"
                     /translation="FWFLGHPEVYIVLLPALGLASEVIATQSRKPIFGYKAMIGSMLV
                     IAFLSFIVWGHHMFITGMNPFLGSVFVFTTLLVAIPSAVKVFNYLATLWRGNIVLTPA
                     MLFAIGLVSFFISGGLTGIILGDSALDINVHDTYFVVAHFHIVMGSSAIFGMFAGIYH
                     WFPRMFGRMMDKKLGYLHFWLTLVGVYGTFFPMHFIGLSGAPRRYYAYTAYEGFDAAL
                     NINVLVSIFAILGGLAQFIFVFNFFYSMYRGRKASQNPWKSNTLEWTAPVEHLHGNWP
                     GEIPTVHRWAYDYGKPNADDDYIPQTTPYSQTTKSNLPGEG"
BASE COUNT          693 a          645 c          658 g          460 t
ORIGIN      
        1 ggagccggaa aaggtaatgg ttctcgtcgt tcacgacaaa gactgctgaa cggaaattgg
       61 aacgcatcac gaaaaacaat ccgatgcaac agccgatcag gataccaacc aacagatcgg
      121 tgagcaggat ggccaagact gttacgagaa agggtacgaa ctggtcccag ccattttgat
      181 acagggagcg gaagatggaa ggttttgcaa gcttataacc ggtatagatc agaatcgccg
      241 ccagggaggc tttggggatc atgttcaaca actccggaat caaggccaca ctcagcaaca
      301 ggaaaactcc atgaaagatg gccgcccatt tggtcctggc acctgcgttg atattggcag
      361 atgtgcgtac gatcacggaa gtcaacggta aaccgcccaa catcccactc acgatgtttc
      421 caaccccctg cgctttcaat tcacggttgg tgggggtcac gcgcttataa gggtccagtt
      481 cgtcggacgc ttcgatacac aacaaggact ccaggctggc tacgatcgcg agcgtaaccc
      541 cggtaatcca aacatccaca ttcgtcagga actgtacatc cgggaaaatg aaaaaggaaa
      601 agaagccgct catggaagta gcgaccggga tttccaccaa gtgctggctc tgcaaggcga
      661 gctcggggaa ctgccgggtt ccccatagat gcaaccaggt cccgatcaat accactacca
      721 gcggcgcggg taaaatccgg aatagtccac cgcgattccc caggaatttc tcccaaagga
      781 tctgcaccag caacgaaacg ataccgatca aggccgccca ggggaggatg tatccgactg
      841 actgaatcag gtcggaaaag gtgttgttcc cgtcgtgcga aaaagactca tccccttcga
      901 agtccgcatc gtatccaacc aggtgcggaa gctgcttgag gatcaggatc aacccgatgg
      961 ctgccaacat tcctttgatg actgaggagg gaatgtaatc acccaataca ccggccttca
     1021 acatacccag aagcaactga ataactcccc cgatcacgac agacaacaga aaagcctcat
     1081 aaaccggcat ctttccgatc gcggcggcta caatggcggt gagcccggca gcaggaccag
     1141 aaacagagag ctgcgaacca ctcagggttc cgatcacaat gcctccgaca atgcctccga
     1201 tcaagcccgc gaacaaggga gcctgtgaac ctaatgcaat gcccaggcaa agcggaaggg
     1261 caactaagaa gactacgagc gcggcgggta gatccgcgct aagaacaggt aaaacggtgc
     1321 tttttccagt acttttcact tgaaatggat taagggaagt ccctaaagat atgaaaatcg
     1381 tagtggggct cccctaacag taagcaatac ttaacaaaca ttaagtatta ctatcatttc
     1441 atgaaacaaa aaagggcaga ttccaactct gcccttttcg attctttcga ttgtgtatga
     1501 tcagccttct cccggcagat tcgacttcgt cgtctgtgaa taaggggtcg tctgagggat
     1561 gtaatcatca tccgcgttcg gcttaccgta atcgtacgcc cagcggtgaa cggtcggaat
     1621 ctcaccgggc cagttgccat gcaggtgctc caccggcgcg gtccactcta gcgtattcga
     1681 tttccacggg ttctgtgacg ccttgcgtcc gcggtacatg ctgtagaaga agttgaacac
     1741 gaagatgaac tgcgcgagac cgccaaggat cgcgaagatg ctgaccagta cgttgatgtt
     1801 caaggctgca tcgaaccctt cataggccgt atatgcatag taacgacggg gagcgccgga
     1861 gaggccgatg aagtgcatcg ggaagaacgt gccgtatacg cccaccagcg tcagccagaa
     1921 gtgcagataa ccgagcttct tgtccatcat gcgaccgaac atgcgcggga accagtgata
     1981 gataccggcg aacatcccga agatggccga tgagcccatt acgatgtgga agtgggcaac
     2041 gacgaaatag gtatcgtgaa cgttgatgtc cagcgcggag tcacccagga tgataccggt
     2101 cagaccgccc gaaatgaaga acgataccag accgatggcg aacaacatgg cgggagtcag
     2161 cacgatgttt ccacgccaga gtgtcgccag gtagttgaag acctttaccg cggaaggaat
     2221 agcgaccagc agggtcgtga atacgaacac cgatccgagg aacgggttca tacctgtgat
     2281 aaacatgtgg tgaccccaaa cgataaacga gaggaacgcg atcaccaaca tggagccgat
     2341 catggccttg taaccgaaga tcggtttgcg ggactgggtg gcaatgactt cggaagccag
     2401 accgagcgcc ggcaacagta cgatatatac ttccgggtga cccaggaacc agaaga
//
LOCUS       DAOTON010000422         2456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33380_length_2456_cov_2.948355, whole genome shotgun sequence.
ACCESSION   DAOTON010000422 DAOTON010000000
VERSION     DAOTON010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33380_length_2456_cov_2.948355"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..215)
                     /locus_tag="P5292_08600"
     CDS             complement(72..215)
                     /locus_tag="P5292_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39224.1"
                     /translation="MQEEIIEIRPLTEASDEDNGMEMSMDEDINMEDSGGMNDFDEDE
                     DDF"
     gene            214..570
                     /locus_tag="P5292_08605"
     CDS             214..570
                     /locus_tag="P5292_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39225.1"
                     /translation="MEITFGCSNGISGDFTGFGAAGFFAAGFGAAGFLAAGFLAAGFF
                     AAGFFAAGFFAAAFFAAGFLEAAFFLATGFLAAFFATFFRAGAAFLAFLAGAFLALAF
                     AFFAGFFLVAMVRWVL"
     gene            720..1991
                     /locus_tag="P5292_08610"
     CDS             720..1991
                     /locus_tag="P5292_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HRS39226.1"
                     /translation="MSTMNQSLPVAEALRVKDLIRRFEEKRPEIVFEWKDAETEAEGW
                     IVINSLRGGSAGGGTRMRKGLDKREVESLAKTMEIKFSVSGPPIGGAKSGINFDPSDP
                     RKQGVLERWFKAVMPLLKTYYGTGGDLNVDEIHDVIPITEKLGLRHPQEGTVVAHFNA
                     GEEQKARIIGQLQRGVKKRLEDLAYSPELTRPITVADMITGYGVAVSVMHYYRLWGGS
                     VKGKRAIVQGWGNVGAAAGYYLAQMGVSIVGIIDRQGGLIKASGYSFEEVRELFLNRQ
                     GNQLNAEGIIPFDKINQEIWQTGAEIFVPAAASRLVQREHLDAMLSKGLEVIASGANV
                     PFADKEIFFGPIMEFADNNCSVIPDFIANCGMARVFRYLMNDETPITDEAIFHDVSEC
                     IGGALEAVYHRNPGKRGLSQTAIEIALEKLI"
     gene            2005..>2456
                     /locus_tag="P5292_08615"
     CDS             2005..>2456
                     /locus_tag="P5292_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39227.1"
                     /translation="MTGKMLVFPVVYFAMFSLRSVLNQTFLGNTLESYLWFLGILLAG
                     LALKRFISKLIARLFYRVVRKQGHRVGSEKFLALTAAPVGFLFLLLILYVACDRLHFP
                     EEWKLVPENVFGLRFVLHQLFEGAVVVAVTWIVLRLVDFFGLVLIERAR"
BASE COUNT          465 a          624 c          715 g          652 t
ORIGIN      
        1 ctgaacgacg caagagctga taattaaaaa ggccaccatt cggcggcctt ttgtatttaa
       61 gtcagtaggg atcagaaatc gtcttcgtct tcgtcgaagt cattcatacc accggagtct
      121 tccatattga tatcctcatc catggacatt tccatgccgt tgtcctcgtc acttgcctcg
      181 gtcagcggac ggatctcgat aatctcttcc tgcatggaaa ttactttcgg ttgttcgaac
      241 ggaatttcgg gcgactttac cggtttcggc gcggcgggtt ttttcgcagc gggtttcgga
      301 gccgctggtt ttttagccgc tggttttttg gctgccggtt ttttcgcggc tggtttcttt
      361 gctgcgggct ttttcgctgc agcttttttc gcagccggtt ttttagaagc cgctttcttc
      421 ttggcgacgg gttttttggc cgctttcttt gcgacttttt tccgggccgg tgcggccttc
      481 ttggctttct tggccggagc ctttttagcc ttggctttcg ccttcttcgc gggcttcttc
      541 ttggttgcca tggtgcgttg ggttctttga agtggtggta ggcaaactta tttccgtgaa
      601 tccaaaatta caagaacaat tattcaccat acaaccgatt ccggaaaaaa aatcgtggac
      661 ccggtcgggg tttgccttgg ttgtccggtg cggctatttg ccgtaggttt gccccgccaa
      721 tgagtaccat gaatcaatcg ttgcctgtcg ccgaagccct ccgggttaag gatctgatcc
      781 gtcgttttga agaaaagcgt ccggagatcg tatttgaatg gaaagatgct gaaaccgaag
      841 cggagggttg gatcgtcatc aactccctgc ggggtggttc cgccggtggc ggtacccgga
      901 tgcggaaggg cctggacaag cgggaggtag aatccctcgc aaaaacgatg gaaatcaagt
      961 tttctgtcag tggccctccc attggcgggg cgaagtccgg gatcaatttc gatccctcgg
     1021 acccccgcaa gcagggggtc ttggaacgtt ggttcaaggc ggtgatgccc ctgttaaaga
     1081 cctactatgg tacgggcggc gacctgaacg tggacgagat ccacgacgta attccgataa
     1141 cggaaaagct gggactccgg cacccgcagg agggaacggt cgtagcccac ttcaatgccg
     1201 gggaggagca aaaggcccgg atcatcggcc agcttcagcg cggcgtgaaa aagcgcctgg
     1261 aggacctggc ctattcgccg gaactcaccc gacccattac cgtggcggat atgatcaccg
     1321 gctatggggt ggctgtttcg gtcatgcatt attaccgctt atggggcgga agtgtcaagg
     1381 gcaaacgcgc aattgtgcag gggtggggta acgtaggggc cgccgcgggc tattacctcg
     1441 cccagatggg cgtttcgatt gtcgggatca tcgatcgtca gggcggtctg atcaaggcat
     1501 cgggctattc tttcgaagaa gtacgggaat tgttcctcaa tcgtcagggt aatcagttga
     1561 acgcggaggg aatcataccg tttgataaga tcaatcagga aatctggcag accggtgccg
     1621 aaattttcgt tccggctgcg gcatcccgcc tggtacaacg cgagcacttg gatgccatgc
     1681 tatccaaggg gctggaagtg atcgcatccg gagccaatgt cccgttcgcg gacaaggaga
     1741 tcttcttcgg accgatcatg gagttcgccg acaacaactg cagtgtgatt ccggatttca
     1801 tcgcgaactg tggtatggcg cgggtattcc gctatcttat gaatgatgaa acgccgatca
     1861 ccgatgaggc catcttccac gatgtttccg aatgcatcgg cggcgccttg gaagccgtgt
     1921 atcatcgcaa tccgggaaaa cgtggattga gccagacggc cattgagatc gcgctggaaa
     1981 agttgatctg ataagctgtt aactttgacc gggaagatgc tcgtcttccc ggtcgtttat
     2041 tttgccatgt tctctctccg ttccgtcctg aatcaaacct tcctgggtaa cacgctggag
     2101 agttatctct ggttccttgg tatcctgctg gccggactcg ccctcaagcg ttttatctcc
     2161 aagctcattg cccgcctgtt ctatcgcgtc gtgcggaagc agggccaccg ggttggatcc
     2221 gaaaaattcc tggcgctgac tgcggcgccg gttggattcc tcttcctgct cctgattctt
     2281 tatgtggctt gtgaccgcct gcattttccg gaggagtgga agctggttcc ggagaatgtc
     2341 ttcggcctgc gtttcgtgct gcatcagttg ttcgaaggcg ccgtcgttgt cgccgtaacc
     2401 tggattgtgc tgcggttggt cgactttttc ggactggtcc tgatcgagcg cgcgcg
//
LOCUS       DAOTON010000423         2454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33411_length_2454_cov_2.767403, whole genome shotgun sequence.
ACCESSION   DAOTON010000423 DAOTON010000000
VERSION     DAOTON010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33411_length_2454_cov_2.767403"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="P5292_08620"
     CDS             <1..1543
                     /locus_tag="P5292_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRS39228.1"
                     /translation="RTATHEVGHWLNLRHIWGDDGTACSGSDQVSDTPNQASEKYGCP
                     SYPSTDACTSTSPGIMFMNYMDYTDDACMNMFTAGQSSRMNALFATGGARVSLLTSLG
                     CTPPSGGGSCGTPSGLASSSITTTSATVRWAAVSGASSYNVQYRVNGTTTWTTVTSTT
                     NSRSLTGLSANTTYQYQIQAVCSGTSGTYSSISTFTTATATTCGTPASLTSSSVTTTS
                     ATVSWGAVSGASSYNVQYRVNGTTTWTTVTSTTNSRSLTGLTANTTYQYQVQAVCSGV
                     SGSYSSIATFTTQATSGCTDIWESNNTISTAKTISTNTDITGQISSTTDKDYFKFTTT
                     SPNTYIRINLTNLPADFDIRLYNSSGTQLAISQNGSTTSEQIIRNTTTAATYYIQVYG
                     YNGANSTTVCYNLRVNVGSTAFRLGDSAEDQLEPVANFNDFVLFPNPAKDQVNLTFNS
                     GVNEVVSVRVMDMVGKVARQLNLAVEQGDNKFSIDVADFSKGIYFVELTNGNERIVKK
                     IIIER"
     gene            complement(1618..2037)
                     /locus_tag="P5292_08625"
     CDS             complement(1618..2037)
                     /locus_tag="P5292_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRS39229.1"
                     /translation="MKNVMLLCGSLLLISGALAQEPSQRVKAGFGTKVTQVKPEYPGG
                     PDSLTSFLTNNLAYPAQAKTERIQGRVYIAFLIDRNGKLQNVRLLSGVHPLLDEEALR
                     VVRSMPDWKPGTVAGKPTDVQYILPIDFIIPKPESKD"
     gene            2137..>2454
                     /locus_tag="P5292_08630"
     CDS             2137..>2454
                     /locus_tag="P5292_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39230.1"
                     /translation="MRIPLYRIASLGLLALATWLFLSPSDRLHTRQEDEKESFSMAWH
                     ALQFLGNSAAYPNADIPPDAFAKAYQFYRENYSGRNFRSTQVADWVSEGPTNIGGRTS
                     GFAI"
BASE COUNT          588 a          728 c          584 g          554 t
ORIGIN      
        1 tcgtacagct acccatgaag ttggtcactg gctcaacctg cgccacatct ggggcgatga
       61 cggtacagcc tgttccggtt ccgaccaggt cagcgatacg ccgaaccagg ccagcgagaa
      121 atacggctgt ccctcctatc cttctaccga tgcttgcacc agtacctctc ccggtatcat
      181 gttcatgaat tacatggact acacggacga cgcctgtatg aacatgttca ccgccggcca
      241 atcgtcccgc atgaatgcct tgtttgctac cggcggagct cgtgtgagcc tgcttacctc
      301 actcggttgt acccctcctt cgggtggcgg tagctgcggc acgccttccg gacttgcttc
      361 ctcttccatt acgactacca gtgcaacggt acgttgggca gcggtaagcg gcgcttcgag
      421 ctataacgtt caataccgtg taaacggtac caccacctgg accaccgtaa ccagcacgac
      481 caactcgcgc tcactcaccg gcctttccgc taataccact tatcagtacc agattcaagc
      541 ggtttgctcc ggtacatcgg gcacctattc gagcatcagc accttcacaa cggcaacggc
      601 taccacctgc ggcacgccag ccagcctgac ttcttcgtcg gtcaccacca cttcagctac
      661 cgttagctgg ggtgccgtaa gcggagcttc cagctacaat gtccaatacc gtgtgaacgg
      721 taccaccacc tggactaccg tgacgagcac cacgaactcg cgatcactca ccggactcac
      781 cgccaatacg acctaccagt atcaggttca agcggtctgc agtggcgtaa gtggttctta
      841 ttcttcgatc gctacgttca ccacgcaggc aaccagcggc tgcaccgaca tctgggaatc
      901 caacaatacc atcagcacgg ctaaaacgat ctcgaccaat accgatatca ccggacagat
      961 cagctcgaca acggacaaag attatttcaa gttcaccacg acgagcccga acacttacat
     1021 ccgcattaat cttaccaacc tgccggccga tttcgatatc cgtctttaca acagttcggg
     1081 tacgcaattg gcgatctcgc agaacggcag caccaccagc gaacagatca ttcgcaacac
     1141 gaccactgcc gctacttatt acatccaggt ttacggatac aatggcgcta atagcaccac
     1201 cgtttgctat aacctgcgtg tgaatgtcgg cagtacggcc ttccgtctgg gcgacagcgc
     1261 cgaggaccag ttggagcctg tggccaattt caatgatttc gtactcttcc cgaacccggc
     1321 aaaagaccag gtcaacctga ccttcaattc cggtgtcaac gaagtggtga gcgtacgtgt
     1381 catggatatg gtcggcaaag tagcccgtca gctcaacctc gctgtcgaac agggcgacaa
     1441 caaattctcg atcgatgttg ctgacttctc gaaaggaatt tatttcgtag aactcaccaa
     1501 cggcaacgag cgtatcgtga aaaaaataat catcgaacga taagccgcta acgttagcag
     1561 taataaaaaa ggtctcgttt gcctgggcaa acgagacctt ttttattgga gcaaatttca
     1621 gtccttgctt tcaggctttg ggataatgaa atcgatggga agaatgtact gcacatcggt
     1681 cggcttaccg gccacggtac cgggcttcca atccggcata ctgcgcacga cacgcaaggc
     1741 ctcttcatca agcagtggat gtaccccgct caacaggcgg acgttctgca gtttcccatt
     1801 tcgatcgatc aggaacgcga tgtaaactct gccctggatc cgctccgtct tcgcctgggc
     1861 cgggtacgcg aggttattgg tcagaaagga tgtaaggctg tccggaccgc cggggtattc
     1921 cggtttcacc tgggtaactt ttgttccaaa acctgccttt acacgctggg aaggctcctg
     1981 ggctaaggcg cccgaaatca gcagcaggct gccgcataaa agcatcacgt tcttcatgca
     2041 ggggagtatt ctttgaggtt agaatcggaa agttaatgaa tacctgcaag ttacccaacc
     2101 ctggttttta tctttgaatt gcagaaaccc ttaccgatgc gtatcccact ctaccgaatc
     2161 gcttcccttg gcttgcttgc cctcgcgacc tggctgtttt tgagtccgtc cgatcgattg
     2221 catacaagac aagaagacga aaaggaatcg ttcagcatgg cctggcatgc gctccaattt
     2281 ctgggaaaca gcgccgccta tcctaacgct gatatcccac cggacgcctt tgccaaggct
     2341 tatcaattct accgcgaaaa ctattccggg agaaattttc gcagcacgca ggtggcggat
     2401 tgggtttccg aaggaccaac caacatcggt ggcaggacca gcggctttgc catc
//
LOCUS       DAOTON010000424         2449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33525_length_2449_cov_1.853383, whole genome shotgun sequence.
ACCESSION   DAOTON010000424 DAOTON010000000
VERSION     DAOTON010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33525_length_2449_cov_1.853383"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2102)
                     /locus_tag="P5292_08635"
     CDS             complement(<1..2102)
                     /locus_tag="P5292_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39231.1"
                     /translation="MKNLNTHVVSPARLVLLATRTTTFVIMSLLLLLGTDASAITYYS
                     RQTGPWSVNSTWSTVGYGNATNTGTRPGPGDVVNIGNGHTITINNTASCATLNIGQGT
                     SGILEFLSTSTYTLTVTGNVTVNTGAILYYNTAVNRVHRMIVGGNFANFGRVDFYVAA
                     NQVVDLTFNTTANSSITGIGTWDLNNVSLTKTGATTATLTVNTSNFEAGLRNFIGTQG
                     TYVHNNTGSFSINPTTATFTIGPNMVFKVPMGTMWFASAADNLILQGELYVNGGNVRV
                     GTTAGLQGLRSDQSGTKIPYLEVSSGTLTVYGGITYGSGSSLEPFSFRMTGGTILLNS
                     GTTGTNRQVFMVNDRTTSSFVMSGGTITLQKPNITGVTTVDFHLCGTGTVTSTGGTIQ
                     FGNASTAAGARFGFRTFATVTMPHFRVTGPAANTITLAPSAGSTTNFRLLSLYIDSGK
                     IFDIRSLSGATADTKTMTLMRTVNGVDALYNSGTFTARSSTVTFNTSGAQAIGGTVTT
                     TFYNLQINNASHITLNRPANVSNLLSMVNGKLLTTNTNILVCQANANANIGTSASYVD
                     GPMEHTLASAATVTKIFPIGKASAYRPVTLQLTHTNASSVSYRGEIFNSSASGLPYSL
                     PPTIANVSNVRYVRFTRSAVSNFSNGKITMYYDTDDGVADKNTLLVAHDNGTSQWSNL
                     GGTATNNWTGSITSGTFNA"
BASE COUNT          516 a          580 c          768 g          585 t
ORIGIN      
        1 gcattgaagg ttcccgaggt gatgctgccg gtccagttgt tcgtagcggt accgccgagg
       61 ttggaccatt gcgaagtgcc attgtcgtgc gccacgagca gcgtgttctt gtcggctaca
      121 ccgtcgtcgg tatcgtaata catcgtgatc ttgccgttgc tgaagttgct gactgccgag
      181 cgggtgaagc gtacataacg aacgttagac acgttggcga tcgtgggcgg aagcgagtag
      241 ggaagaccgg aagcggacga gttgaagatc tctccccggt agctgacgct ggatgcgttc
      301 gtatgcgtaa gctgtaaggt aaccggacgg taagcgcttg cttttccgat cgggaagatc
      361 ttggtcaccg tagccgcgct ggccagggtg tgttccatcg gtccgtcgac gtacgaagcc
      421 gaagtgccga tgttggcatt ggcgttcgcc tggcaaacaa ggatgttcgt attcgtcgtc
      481 aggagcttcc cgttgaccat ggagagcagg ttgctgacgt tggcaggacg gttgagcgtg
      541 atgtggctcg cgttgttgat ctgaaggttg tagaaggtgg tcgtaaccgt accgccaatg
      601 gcttgtgcgc cgctggtgtt gaaggtaacg gtactgctgc gcgcggtgaa ggtcccgctg
      661 ttgtacaggg catccacgcc gttgacggta cgcatcagcg tcatggtctt ggtatcggct
      721 gttgcgccgg acaggctcct gatgtcgaag atctttccgg aatcaatgta caggctcagc
      781 aatcggaagt tcgtggtaga accggcggac ggagccagcg tgatcgtatt cgcggccggg
      841 ccggtgacac ggaaatgcgg catggtgacg gttgcaaagg tccggaagcc gaagcgtgcg
      901 cccgcagccg tgctggcatt gccgaattga atggtaccgc cggtgctggt gactgttccg
      961 gtgccacaca ggtggaaatc gacagtcgtt acgcccgtga tgttcggctt ttgcaacgtg
     1021 atcgttcctc cgctcatcac gaaggaactg gtcgttcggt cgttgaccat gaagacctga
     1081 cggttcgtgc cggttgttcc actgttcaat aggatggttc cgccggtcat gcggaaggaa
     1141 aacggctcca gactggaccc gctgccgtag gtgataccgc cgtaaacggt cagggtaccg
     1201 gaagaaactt cgaggtaagg gatcttggta ccgctctggt cggaacgaag accctgcaag
     1261 cctgcagtgg tacctacacg gacattgcca ccattcacat acaattcgcc ttgcaggatc
     1321 aggttgtccg cagcggaagc gaaccacatc gtgcccatgg gtactttaaa taccatgttc
     1381 ggtccgatgg tgaacgttgc cgtagtcgga ttgatgctga aggaaccggt attgttgtgt
     1441 acataggtgc cctgggtacc gatgaaattc cgaagccctg cttcgaaatt gctggtattg
     1501 accgtcagcg tggcggtggt agcaccggtt ttagtaaggg acacgttatt gaggtcccag
     1561 gtaccgatac ccgtgatact cgagttggca gtagtattga atgtcaggtc tactacctga
     1621 ttcgcggcaa catagaaatc aacgcgaccg aagttggcga agttgccccc gacgatcatg
     1681 cggtggacgc ggttgacggc cgtattgtaa tagaggatgg caccggtatt gacggtcacg
     1741 ttgcccgtta cggtcagcgt gtacgtactg gtggaaagga actccaggat tcccgaagtg
     1801 ccttgtccga tgttcagcgt ggcacagctg gcggtgttgt tgatggtgat cgtgtgaccg
     1861 ttgccgatgt tgaccacatc gccgggacct ggtctggttc cggtattggt cgcgttgcca
     1921 tagccgacag tcgaccaggt ggaattgacc gaccagggtc ctgtttgcct tgaataatac
     1981 gtaatggcac tggcatccgt tccgagcagg agcagcaggg acatgatcac gaacgtggtg
     2041 gttcgtgttg ccagaagcac caggcgcgca ggggatacta cgtgagtgtt cagatttttc
     2101 atggggcaga atttttggct caaagtgtaa atgcggactt tggccgcgtt cgtctaattt
     2161 aatcggatgg ggagggggag agttcccgca ataactacag gtgattgcag ctgtaagaca
     2221 ttgaaagaaa agaggacaat tgaccgtcga ggcccggtat ttcgacggat ttgaacgggc
     2281 atccgcattt ccggaaaaat aaatcgtatc ggtttttgcc gctgggccgg acgaattcgg
     2341 atagtttgaa aaatacttac agccttgaac aacccattcg ttcgcgtgtt ttacgtaaat
     2401 ttgaattcca cttagcacgc tatgccccag tatcatcagt tgggtcagt
//
LOCUS       DAOTON010000425         2440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33691_length_2440_cov_3.192453, whole genome shotgun sequence.
ACCESSION   DAOTON010000425 DAOTON010000000
VERSION     DAOTON010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33691_length_2440_cov_3.192453"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="P5292_08640"
     CDS             <1..1102
                     /locus_tag="P5292_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRS39232.1"
                     /translation="GGGRCNVTHACFDPALLTGFYPRGGKELRQVFSRFQPRDTVSWF
                     EREGVRLKAEEDGRMFPDTDQSETIVRCLEEAAQHAGVVVRKQSAVLAINPDPNGGMV
                     LQLADGSLRADKVLVASGGMPSASGFAWLKDVGHAIQDPVPSLFTFNLPQDPLTGLPG
                     LVVDPAEVKLIGTKFKETGPVLVTHWGLSGPAILRLSARAARELAAVHYRQEILINWL
                     PGQNEQQLREIFSANRHQFGTRMLRSHTFEGLPKRMWEYLLVCCEIPEQATWSELKST
                     EIQRLCDKLLRDRHLLNGKTTFKDEFVTCGGVKLKEVDFRTMESRLVPGLYFAGEVLD
                     IDGLTGGFNFQAAWSTGFIAGKSMAESSSALH"
     gene            1128..>2440
                     /locus_tag="P5292_08645"
     CDS             1128..>2440
                     /locus_tag="P5292_08645"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS39233.1"
                     /translation="MRSILTFDILNQQHCMTNSIRVIWISIFLLFSVTGQAQYTLSGI
                     VSDKSDKTKLIEGATLFIPEFNRIDLSKEGGTYIFRNLHQGRINVQVTRAGYRSRLVQ
                     VSLKDSATVVNVEMEPALIDLQEVTVTGNRERLPDMVPYALSTMTRDELERQGAVHVL
                     SALAYQPGIDKITLGNGIQKPVIRGLSFNRLLIYQYGTRIENQPWDDRHDMGVNENGV
                     DQVEVLKGPAALMAGPDALAGAIIFTDEKPAALGTTSGDVDLGFFTNTLGIRGRAGVK
                     GMNNNGLFYSFRGGWQSHTSYIQGEGEERQKNEIDKDFAPNSKFGSTDAKLVLGTSKR
                     WGVSKLTYSYLRQQIGIVEQEDESQYLEPEQFTEEQRDREFEAPYQDVTNHIVSSENS
                     ILLPGGRINANLSFQHNDRKEFEPTADGGKEKAIGLRLNTLTYDLR"
BASE COUNT          595 a          617 c          697 g          531 t
ORIGIN      
        1 cggaggcggt cgctgtaatg tcacccacgc ctgcttcgat ccagccttac tgaccggttt
       61 ctacccgcgt ggcggtaagg aactgcggca agtattcagt cgcttccaac cgcgggatac
      121 cgttagttgg tttgaacggg aaggtgtgcg gttgaaggcc gaagaagatg gtcgtatgtt
      181 tccggatacg gatcaatctg aaacgatcgt gcgatgcctg gaggaggcgg cacaacatgc
      241 aggtgtggtg gtcaggaagc agtctgcggt actggctata aatccggatc caaacggtgg
      301 aatggtgtta cagcttgctg acggttcact gcgcgccgac aaggtcctgg tcgcaagtgg
      361 cggaatgccg agtgcatccg gtttcgcctg gctgaaagac gttggccacg cgatccagga
      421 tccggtgccc tccctgttta cattcaatct cccgcaagat cccctgacag gattgccggg
      481 actggtcgtt gatcctgctg aagtgaaatt gatcggtaca aagttcaaag agaccggacc
      541 ggtcttggtc acgcattggg gattaagcgg accggcgatc ctgcggctca gtgcccgggc
      601 cgcccgtgaa ctggccgcag tgcattaccg tcaggaaatc ctgatcaact ggctgccggg
      661 tcagaacgaa cagcaattac gggagatctt ttccgccaat cgccatcagt tcggaacccg
      721 tatgcttcga tcacatacat tcgaaggatt gccgaaacgg atgtgggagt atttactggt
      781 gtgctgcgag atacccgaac aggcgacctg gtcggagttg aagtcgaccg aaatacaacg
      841 actctgcgat aagctgttgc gcgatagaca tctgctcaac gggaagacta ccttcaagga
      901 tgaatttgta acctgtggtg gcgttaagct gaaggaagtg gattttcgaa cgatggaaag
      961 ccggttggtg cctggacttt atttcgccgg cgaagtactc gatatcgatg gtctgaccgg
     1021 aggatttaat tttcaggcgg cctggagtac cggtttcatc gccgggaagt caatggccga
     1081 aagttcatcc gcacttcatt aaaacgaaat ctatttcaga agcgccaatg cgctcaatcc
     1141 ttaccttcga catcctgaac caacagcatt gcatgaccaa ctccatccgc gtaatctgga
     1201 tttctatatt cctgctattc tccgtgacag ggcaggcaca gtacaccctg agtggaatcg
     1261 tctccgacaa gtccgacaag acgaagttga ttgaaggcgc aacgctgttc atcccggaat
     1321 tcaaccgcat cgatctgtcc aaagaaggtg gcacctacat tttccgtaac ctccaccagg
     1381 ggcgaatcaa tgtccaggtc acccgcgccg ggtatcgtag ccggctggta caggttagtc
     1441 ttaaggattc cgctacggtc gtgaatgtgg agatggaacc tgcactgatt gacttgcagg
     1501 aggtcaccgt gaccggaaat cgcgagcgac taccggatat ggtgccctac gcgctcagta
     1561 ccatgactcg tgacgagttg gagcgccagg gtgcagtgca tgtattatcg gctttggctt
     1621 atcaaccggg aatcgacaag atcaccctgg gaaatggaat acaaaagccg gtgattcgcg
     1681 gcctctcgtt caaccgactt ttgatttacc aatacgggac acggatcgag aaccagccgt
     1741 gggacgatcg gcatgatatg ggcgtaaacg agaacggagt cgatcaggtg gaagtactga
     1801 aaggccccgc ggctttgatg gccggtcccg atgcgctggc cggtgccatc atcttcacgg
     1861 atgaaaaacc cgcggccttg gggacaactt ccggcgacgt cgacctgggg tttttcacca
     1921 atacactggg aatacgcggc cgtgcaggag tgaaggggat gaacaataac ggccttttct
     1981 acagtttccg tggaggctgg cagtcgcata cgagttacat tcagggtgaa ggtgaagaac
     2041 gccagaagaa tgagatcgat aaggatttcg ctcccaacag caagttcggt tccaccgatg
     2101 ccaaactggt cttgggaacg agcaagcgct ggggcgtttc caaactgaca tactcctacc
     2161 tgcgtcaaca gatcggtatc gtcgagcagg aggatgagag tcaatacctg gaaccggaac
     2221 agtttaccga ggaacaacgg gatcgcgaat tcgaagcacc gtaccaggac gtaactaatc
     2281 acattgtctc aagtgagaat tcgatcctct tgccaggggg taggatcaat gccaatcttt
     2341 ctttccaaca caatgaccga aaggagtttg aaccgaccgc ggatggtggg aaagagaagg
     2401 caatcgggct gcgtctcaac accctcacct acgatctgcg
//
LOCUS       DAOTON010000426         2437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33752_length_2437_cov_2.808144, whole genome shotgun sequence.
ACCESSION   DAOTON010000426 DAOTON010000000
VERSION     DAOTON010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33752_length_2437_cov_2.808144"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="P5292_08650"
     CDS             complement(<1..927)
                     /locus_tag="P5292_08650"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRS39234.1"
                     /translation="MNRLLCLLLICIIGQIRLSAQGILDTLGNTIVRIETAVDSAQFA
                     GGYASGPWDLHLGPDGQLWFTDQTAIRRWHPVTGVISTVLRVPSGYFMGLAHHSDFLH
                     QPYVYATIDTGYYYGQSTRIEVWRYRYNSAVDSLEDPLFLLSWYHPGEHSGGRMVFGQ
                     DSCLYIGTADYAGLQWQDTLFMNSGKVLRVRPDGSVPSDNPRPDYSWTWGHRNPQGIV
                     QVPNGNIIVSEYGQINDELNLIRRGNHYGWIIFDGNQCIGSIDTCNAYQSSLTFPIDV
                     GQNPPSGITWYDHPSIPELHGLVQAVTGINQGL"
     gene            1182..1550
                     /locus_tag="P5292_08655"
     CDS             1182..1550
                     /locus_tag="P5292_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRS39235.1"
                     /translation="MSHRIVHFEIPCNQPEKTIRFFEEAFDWKFQKFGEEDYWLAITG
                     NDEGPGINGAVMRKRDPNQPIVNSIDVPDMDAAIRRVEGAGGLIVVPKMPIPTVGWLA
                     YFKDPDGNIHGLYQNDPQAR"
     gene            1759..>2437
                     /locus_tag="P5292_08660"
     CDS             1759..>2437
                     /locus_tag="P5292_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39236.1"
                     /translation="MYRHFIAFLIFLFSFQASTSAQWTTVCGAGNGFVDNLEVFDNGL
                     YATGFFTTLCGVTTPYLARYDGTSFQPVGTGLVREGHQLRSIGSDLFFVGYEPGTDSN
                     WVYRWDGTTFQPFGEGVYLTTASPGFSQTANLYAVIDYNGSLVACGEFDRVGARAISG
                     IMRWNGSGWDSLGSGLSGNIPGTPDLLYPHELCLFGADLVVAGNFSHAGGQLVNGIAR
                     WDGTNWFP"
BASE COUNT          545 a          652 c          657 g          583 t
ORIGIN      
        1 gagtccctgg ttgatgccgg tcaccgcctg taccagtccg tgcaactccg gaatcgacgg
       61 atggtcgtac caggtgattc ccgagggagg gttctgcccg acatcgatcg ggaaggtcag
      121 gctggattga taggcgttgc aggtgtcgat gcttcctatg cattgattcc catcgaagat
      181 gatccacccg taatggttgc ctctgcggat gaggttgagt tcgtcgttga tctgtccgta
      241 ctccgatacg atgatgttgc cgttcggtac ctgtacgatt ccctgcgggt tccggtgtcc
      301 ccaggtccag ctgtagtcgg gacgagggtt gtcgctcggc acggaaccgt cagggcgtac
      361 ccgcagcact ttccccgagt tcatgaacag cgtatcctgc cactgtagtc cggcataatc
      421 ggccgtcccg atatacaaac agctatcctg accaaagacc atgcgtccgc ctgagtgttc
      481 acccgggtgg taccaggaaa gcaggaacaa tggatcttcc agtgaatcaa cagccgaatt
      541 gtatcgatag cgccacactt ctatgcgcgt gctttgtccg tagtaatagc cggtatcgat
      601 cgtagcatac acataaggtt gatgtaaaaa atcgctgtga tgcgccagtc ccatgaaata
      661 tccgctggga acacgcaata cggtgctgat cacacccgtg accggatgcc atcggcgaat
      721 ggctgtctgg tccgtgaacc acaattgccc gtcaggacct aagtgcagat cccacggccc
      781 gctggcataa ccgcccgcga actgtgcgct gtccaccgcg gtttcaatcc gtacgatggt
      841 attccccaga gtatcgagga ttccttgagc cgacaaacga atttgtccga tgatgcaaat
      901 cagtaaaagg cagaggaggc gattcatcag gctaagataa cggcttgccg gaatgtggcc
      961 ggaacccgaa tggaagaaga tgttacgcaa actatattcg atttctggtg ctcatgaagc
     1021 aggatcgttt cgcgacccga cttaaaaaag taccagtttt tcgtagaagt ctcttgcaag
     1081 cggaggtggt cctgtcgcat gaatgttaaa ctgtgttgct gaattgaacc gaatccttca
     1141 agccgctatc ttggaatttc accaatgatt ccatgaccgc catgagccac cgcatcgtcc
     1201 atttcgagat cccctgtaac caaccggaga agaccatccg gttttttgaa gaagcgttcg
     1261 actggaaatt ccagaaattc ggcgaggaag attactggct ggccatcacc ggtaacgacg
     1321 aaggtccggg tatcaacggc gccgtgatgc gcaaacggga tcccaaccag ccgatcgtca
     1381 attccatcga tgtaccggat atggatgcag cgattcgccg cgtggaagga gccggtggtc
     1441 tgatcgtcgt tccaaagatg ccaatcccta cggtcggttg gctggcctac ttcaaggatc
     1501 cggatggaaa catccacggg ctttatcaga acgatccgca ggccaggtaa atggcgaagc
     1561 gtcttcctgt tggcaagtca gcacaaggca accgactctg tgagcaatgg tcggttgcgt
     1621 ctgctgaaac gaatggttgt ctggcgactt cggattactt tccgttatcc gcgctatttc
     1681 cgttctttca tcaactacaa ttgcccagtt ttcggcaagt acactctccc gaatcgcgtt
     1741 aaaccagtta cttcaattat gtatcgtcac tttatagcat tccttatttt cctcttcagc
     1801 tttcaagcaa gcactagcgc gcaatggacc acggtatgcg gtgccggcaa tggctttgtc
     1861 gacaacctgg aagtattcga caatggtctt tacgctaccg ggttcttcac caccttatgc
     1921 ggagtgacta cgccttatct agcccgctat gacgggactt ccttccaacc ggtcgggacc
     1981 gggcttgtcc gtgaaggaca ccaactgcgc agcatcggat ccgacctgtt tttcgtgggc
     2041 tatgaacccg gaacggattc caattgggtc tatcgctggg acggaaccac ctttcagcct
     2101 ttcggagaag gtgtttacct gaccacggca tcgccgggct tttcacagac ggccaacctc
     2161 tatgcggtga tagactataa cggctcactc gttgcctgcg gagaattcga tcgcgtggga
     2221 gccagggcga tctccggcat catgcgatgg aatggaagcg gatgggattc gctcggaagt
     2281 ggtttgtcgg gtaacattcc gggcactccc gatctcttgt atccgcacga gctttgtctt
     2341 ttcggagcgg atctggtagt agccggcaac ttctcgcatg cgggaggcca gctcgtaaat
     2401 ggtatcgcac gctgggacgg aacgaactgg ttcccgt
//
LOCUS       DAOTON010000427         2432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33839_length_2432_cov_2.842659, whole genome shotgun sequence.
ACCESSION   DAOTON010000427 DAOTON010000000
VERSION     DAOTON010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33839_length_2432_cov_2.842659"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..919)
                     /locus_tag="P5292_08665"
     CDS             complement(200..919)
                     /locus_tag="P5292_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRS39237.1"
                     /translation="MRGFKNITVLAGLLLVLQWTDSRAQLHTQPLNLGRYDKQKIHFG
                     FSLGINSANFRVNLADDLMNRDSIYRVDPDPVTGLNLGIISNLRIGDHFDLRFIPALS
                     FCQRGLVYDFVYPAGGTATVVKQVESTYLEFPVMLKFKSRRVNNYRVYVLGGGRYSID
                     MVSQAKVKQKDKDIIKLSRNDYGYEIGIGFDFYLEYFKFSPEIKMFQGLPNLLVRENT
                     PYSSPIDALNSKIFFLTFNFE"
     gene            complement(925..1656)
                     /gene="ubiE"
                     /locus_tag="P5292_08670"
     CDS             complement(925..1656)
                     /gene="ubiE"
                     /locus_tag="P5292_08670"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRS39238.1"
                     /translation="MGTTVRPYKESNAAKKDQVARMFNNIAWRYDFLNRFLSLGIDRV
                     WRKKAIRTLRPHQPRQVLDIATGTGDLAIEALKLNPDQVTGVDISVDMLRIGREKLKR
                     KGLSDKIHLLEGDSEKLIFNDNKFDAVTVAFGVRNFEHLQDGLREILRVLRPGGVVMI
                     LEFSQPRNALMNRLYQFYSTRITPWFGRKISKDPQAYTYLHESIAAFPSGQDFLRIME
                     SCGFQKTVVKPMTFGVVSIYTGFKP"
     gene            complement(1656..>2432)
                     /locus_tag="P5292_08675"
     CDS             complement(1656..>2432)
                     /locus_tag="P5292_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRS39239.1"
                     /translation="SVIGSDTNGRDFIELMQQLGLSTEGIVQSPDRPTTVKTRVIGNN
                     HQMLRVDEESDSDIPVAERKQLLGRIESFLDVRKVDVIVFEDYDKGIINKALIEAVVR
                     MARSRHIPVAVDPKKRNFNHYRNVTLLKPNLKELREGMKLDLDKNDLNAISSAVRKLC
                     KDLQLGSVLLTLSEKGILHSDGTEELLVPAHIRNIADVSGAGDTVISVAACCLALGVS
                     PTLLSSLSNLAGGLVCESVGVVPVDKEQLFREAKRLKLGA"
BASE COUNT          552 a          634 c          690 g          556 t
ORIGIN      
        1 ccacgaataa ccgtatgcga acctgtcggc cacgggcatc gatggaacgc ttgcgcagta
       61 ccagccgaat gcgggagggc ggtaccctga gcagcgaagc cgcctgccgc atttgtgccg
      121 attggtctgc cgcctcttcg ggaagaagga ccagctcgag cgaatgaatc atagtgggat
      181 ggcccggaag gcctgaagct tattcgaagt tgaaggtcag gaaaaagatc ttggagttca
      241 aggcgtcgat aggcgaactg tagggagtat tctcccggac aagcaaattc ggaagaccct
      301 ggaacatttt gatctccgga gagaacttga aatattcgag ataaaagtcg aacccgatcc
      361 cgatctcgta gccatagtcg tttcggctga gcttgataat gtccttatcc ttctgcttca
      421 ctttggcttg ggagaccatg tcgatcgagt agcgcccgcc gccgagcacg tatacgcggt
      481 aattgttcac ccgtcgactt ttgaatttca gcatgacggg gaactccagg taggtggact
      541 ccacctgctt cacgaccgtg gcggttccgc cggccgggta cacgaagtca tacaccagtc
      601 cacgctggca aaaagacagt gcggggatga aacgcaggtc aaagtgatcg ccgatcctca
      661 ggttcgagat gatcccgaga ttcaaacccg tcaccggatc cggatcgacg cgatagatgc
      721 tgtcccgatt catcagatcg tcggccaggt tcacccggaa gttcgccgag ttgataccca
      781 ggctgaaacc gaagtggatc ttttgcttgt cgtaacggcc caggttcaac ggctgtgtgt
      841 gtaactgcgc acgggagtcg gtccactgca atacgagcag caagccggcc aacactgtta
      901 tgtttttgaa tccgcgcaaa acgatcaggg cttgaaaccg gtatagatgg aaacgacgcc
      961 gaaggtcata ggcttcacaa cggtcttttg aaatccgcag gattccatga tgcgaagaaa
     1021 atcctgaccg gaagggaatg cggcaatgga ttcgtgaagg taggtgtacg cctgcggatc
     1081 tttggaaatc ttgcgtccga accagggagt gatccgggtt gagtagaatt ggtagaggcg
     1141 attcatcagg gcgtttcgcg gctgggaaaa ttcgaggatc atgacaactc cgccggggcg
     1201 taagacccgg agtatttccc ggaggccgtc ctgcaggtgc tcgaaattcc ggaccccgaa
     1261 agcaacggtc accgcatcga acttattgtc gttgaaaata agcttttccg aatctccttc
     1321 cagcaggtga atcttgtcgc tgagtccttt tcgcttcagc ttctcacgac cgatgcgtag
     1381 catgtcgacc gaaatgtcaa ctccggtaac ctggtcggga ttgagtttca aggcttcgat
     1441 cgcgagatcc cccgtaccgg tggcgatgtc gagtacctga cgcggttgat gggggcggag
     1501 ggtgcgaatg gcttttttac gccatacccg atcgatcccg agcgatagaa agcgattgag
     1561 aaagtcgtac cgccacgcga tgttgttgaa catgcgtgcg acctgatctt tcttcgctgc
     1621 attcgattcc ttatacggtc tgaccgtcgt tcccatcagg caccgagttt gagtcgcttc
     1681 gcttcgcgga ataattgttc cttatcgacc ggaacaacgc ccacggattc gcaaaccagt
     1741 ccaccggcga gattcgaaag gctggacaac agcgtgggcg aaacgcccag tgccaggcaa
     1801 caagccgcca cactgatgac cgtgtcacct gcgccggaaa catccgcgat attccggata
     1861 tgtgcaggca cgaggagttc ctccgttccg tcggaatgca ggatgccttt ttcggagagg
     1921 gtaagcaaga ccgagccgag ctggagatcc ttgcacaatt tgcgaaccgc tgaactaatg
     1981 gcgttcaggt catttttatc aagatccagc ttcatgcctt cccggagttc cttcagattg
     2041 ggtttcaaga gggtaacatt ccggtagtgg ttgaagtttc gttttttggg atccactgcg
     2101 acggggatgt ggcgggatcg tgccatgcgg accacggcct cgatcagcgc tttgttgatg
     2161 atccctttgt cgtaatcctc aaatacgatg acatcgactt tacggacatc gagaaaactc
     2221 tcgatacgtc cgagtaattg cttgcgttcc gcgacgggga tatccgaatc cgattcttcg
     2281 tcgacccgca gcatctgatg gttgttgccg atcacgcgcg ttttaacggt ggtcggacga
     2341 tccggcgact gcacgatccc ttcggtgctg agaccgagtt gttgcatcag ctcgatgaag
     2401 tcccggccgt tcgtatcgct gccgatgacc ga
//
LOCUS       DAOTON010000428         2427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_33937_length_2427_cov_2.599916, whole genome shotgun sequence.
ACCESSION   DAOTON010000428 DAOTON010000000
VERSION     DAOTON010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33937_length_2427_cov_2.599916"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="P5292_08680"
     CDS             complement(<1..525)
                     /locus_tag="P5292_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRS39240.1"
                     /translation="MKQHAPSSPTNTPDFSAFRLEVLNDYRLANESRQASLLGRKEVL
                     TGKAKFGIFGDGKEVAQLALAKQFREGDFRSGYYRDQTLMFAIGESDIEKFFAQLYAT
                     PSLEDEPASAGRSMNGHFATRNLNDDGSWKDLTRQKNSSADISPTAGQMPRLVGLAWA
                     SKLYRNNPGLQAVKG"
     gene            700..1032
                     /locus_tag="P5292_08685"
     CDS             700..1032
                     /locus_tag="P5292_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39241.1"
                     /translation="MTLLQASSSLVIFSGFRLNQDFIAKNLCENRFVPKSHCHGSCHM
                     MKEMKQEEQREERSGGNVQSGGEVVYIPHQATALVSTAGFVTSLPSEYLIGSATAFSE
                     PLCRPPAV"
     gene            1209..1985
                     /locus_tag="P5292_08690"
     CDS             1209..1985
                     /locus_tag="P5292_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39242.1"
                     /translation="MKSNLLKYAILSVFALTTVFSSCKKDEDDDQPTPTTGSLSFELT
                     HKVGSDPLVQNTVTYVNLDGDSFIVTKFRYYISNVVLKKSDGSTYAPSECYYLVDDSV
                     SSSKTFTISDIPVGEYTSMTFLLGVDSTRNCSGAQSGALDALNGMFWSWNNGYIFLKL
                     EGMSASASGGMFMYHVGGFKGTTNAIRTIAPDFNGDVVDMRAGKTSTIHMVANLAELF
                     ANPTTIDIGTLSMIHMPSAEAVTLADNYADMFRVDHIHND"
     gene            1990..>2427
                     /locus_tag="P5292_08695"
     CDS             1990..>2427
                     /locus_tag="P5292_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRS39243.1"
                     /translation="MRLSLRLYRVILLMGAGIVLFVGCGQDESTELQLPSDGDGLTEA
                     DVELRLPAGFPAPYYTTATNPITLDGFRLGRKLFYDPILSRDSTISCASCHQQFAGFS
                     HLDHDRSHGIDGLFGIRNAPSLANLIWKDELMWDGGVNNIEVQP"
BASE COUNT          559 a          634 c          606 g          628 t
ORIGIN      
        1 acccttcaca gcctgcaagc ccggattatt gcggtacagc ttggatgccc atgccagtcc
       61 gaccagtcgg ggcatttgtc ctgcggtagg ggagatatcg gctgatgaat tcttttgcct
      121 ggtaagatct ttccagctac cgtcatcgtt caggttgcgc gtggcaaaat gcccgttcat
      181 ggaacggccc gctgaggcag gctcgtcttc cagactcgga gtagcataaa gttgagcaaa
      241 gaacttttcg atatcggatt ccccgatggc gaacatcagg gtctggtctc tgtaatatcc
      301 ggatcggaag tctccctcgc ggaattgttt cgcaagtgcc agttgcgcca cttcttttcc
      361 atcgccgaag attccgaact tggctttgcc tgtgaggact tcttttctcc ccagcagtga
      421 agcctgtcgg ctttcgttgg cgagacgata atcgttcaac acttccagcc gaaaggctga
      481 aaaatccggc gtatttgtcg gggaagaagg agcgtgctgt ttcatgaaat cggcctaaaa
      541 gtgcccaaaa ccgaacaaat ataacaaatc agggccgaac gattcacaac tgtttcaagt
      601 ataaaaccgt cagaacatga caaaactctt gttttcccgg attgcttcta aggtatattt
      661 gcaacaatgc gttccctact ggcgatactt gtcagcctga tgaccctgct ccaagcgtcg
      721 agctccctgg tcattttttc cggctttcgc ttgaaccagg atttcattgc caagaatctt
      781 tgtgagaacc gcttcgttcc caaatcgcat tgccacggca gttgtcatat gatgaaggaa
      841 atgaagcaag aggaacaacg ggaagagcgc tccggaggaa atgtgcagtc gggcggagag
      901 gttgtttaca ttccccatca agcaacagct ttggtttcca ctgccggttt tgttacatca
      961 cttccgagtg aatatttgat cggatctgcc accgcttttt ctgagccgct ttgtcggcca
     1021 cctgcggtct gaatgcggcc ccggtaatgc acgctttacc ggttattttc tgcttcgtta
     1081 caaccacact attgcatcac agattcagga gcgattcagc ctttgaagct gtctgatcct
     1141 gaatgaatgc aattgtttgg tgaagtctgc agaatccatt cagtttaatc catcaagtat
     1201 ccccttcaat gaaatccaat ctccttaaat acgccatcct gtccgtattc gccctcacga
     1261 ccgttttcag ctcttgtaag aaagacgaag acgacgatca accgacaccg accaccggtt
     1321 cattgtcttt cgaactgaca cataaggttg gttcggaccc tctggtgcag aacaccgtga
     1381 cctatgtcaa tctggatggc gattcgttta ttgtgacgaa gttccggtat tatatctcca
     1441 atgtagtttt gaagaaatca gacggaagta cctatgcccc gtcggaatgt tattacctcg
     1501 tggatgattc ggtgtcaagc tcgaagacct tcacgatttc cgatattccg gtaggcgaat
     1561 atacttccat gacctttttg ctgggagtag acagtacccg taattgctcc ggtgcccagt
     1621 cgggcgcgct cgacgccctc aacggaatgt tctggagttg gaataacggt tacattttcc
     1681 tgaagctgga aggcatgagt gcctccgcct ccggtggtat gttcatgtat cacgtgggtg
     1741 gtttcaaagg aacgaccaac gcgatccgga ccattgcacc tgacttcaac ggggacgtcg
     1801 tcgacatgcg agccggaaag acttccacca tccatatggt agcgaacctt gcggaattgt
     1861 tcgccaatcc tacgactatt gatattggta cactgagcat gatccatatg ccgagcgcgg
     1921 aagcggtcac cctggccgac aactacgccg acatgttccg ggtcgatcat atccacaacg
     1981 actgaccgaa tgcgtctgtc tctgagactt tatcgggtta tcctcctgat gggagccgga
     2041 atcgttctgt tcgttgggtg cgggcaggat gagtccacgg agctgcaact gccctcggac
     2101 ggtgatggac tcaccgaagc cgatgtggag ttgcggctcc cggccggttt ccctgcgccg
     2161 tactatacca ctgccacgaa tccgattacc ctggatggat tccgacttgg ccggaaactg
     2221 ttctacgatc caatactctc acgcgacagt acgatttcct gcgcttcctg ccaccagcag
     2281 ttcgcgggat tctcgcacct ggatcatgac cggagccatg gtatcgacgg actattcgga
     2341 attcgcaatg ccccgagtct tgcgaacctt atttggaagg atgagttgat gtgggatggt
     2401 ggagtgaaca acatagaagt acagccc
//
LOCUS       DAOTON010000429         2416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_34115_length_2416_cov_3.289708, whole genome shotgun sequence.
ACCESSION   DAOTON010000429 DAOTON010000000
VERSION     DAOTON010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34115_length_2416_cov_3.289708"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="P5292_08700"
     CDS             <1..1619
                     /locus_tag="P5292_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRS39244.1"
                     /translation="RIHRIVHRLETEHDNEALLHELHDAQERFAMIDGYQFRQNAATV
                     LEGLGFTTRDLERPFREFSGGWRMRALLARMMLRDPDMLLLDEPTNHLDLPSIEWLEN
                     YLKSYQGTVIVVSHDRWFLDRIVNKIAEIDQRKISLYSGNFSEYIDQKSQRLALQEAA
                     YRNQQKFLEEQNKLIDRFRAKASKASMAQSRIKMLERMEKVEAVDSEAPTIRIRFDAA
                     RQPGKIISHLIIQDKRFGDNVLLHDTEAMIRRGDKIALIGANGKGKSTLLRMINGSEP
                     YDGKAETVYNVDAAFYAQHQLESLHLQNDLLTELQSFAPEKKESELRGILGSFLFSGD
                     DVFKKISVLSGGEKARIALAKVLLSKANFLLLDEPTNHLDMATVSMLVDVLNDYEGSF
                     LVVSHDRHFLSCVANRIWWIEDQHLRTYEGSYEEYEEWKTRQLKAEAVAEKKQPVQQK
                     VEKPKLERSDEEKKQRQRTQKKFDKLEEELEQLRQSRHELENLLARPEVYSDPGKFQD
                     HLDRFNRIDADFQAKTREWEELFEQLSGME"
     gene            1939..2325
                     /gene="apaG"
                     /locus_tag="P5292_08705"
     CDS             1939..2325
                     /gene="apaG"
                     /locus_tag="P5292_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRS39245.1"
                     /translation="MITQVTSGIKVSVETFYRPDQSNPLLNQYVFAYRITITNNSDFS
                     VQLRRRHWFIIDSSATNREVEGEGVVGELPILGTGESYQYVSGCNLDSELGKMYGTYL
                     MERLIDKKQFYVRIPEFQMIVPYKLN"
BASE COUNT          567 a          649 c          667 g          533 t
ORIGIN      
        1 agcggatcca tcggattgtc catcgcctgg aaaccgaaca tgacaacgaa gccctgctgc
       61 atgaattgca cgatgcacag gagcgcttcg cgatgatcga cggttaccag ttcaggcaaa
      121 acgcggcgac ggtattggag ggcttgggtt tcaccacccg cgatctggaa cgaccctttc
      181 gggagttcag cggcggctgg cggatgcgcg ccctgctggc gagaatgatg ctgcgcgatc
      241 cggacatgtt gctgctcgat gagccgacca accacctgga tcttccatcc atcgaatggc
      301 tggagaacta cctgaagagt taccagggaa cggtgatcgt ggtttcccac gaccgctggt
      361 tcctggatcg cattgtcaat aagatcgccg aaatcgacca gcgaaagatc tcgctctatt
      421 cgggtaattt ttccgagtac atcgaccaga aatcgcaacg cctcgctttg caggaagcag
      481 cctaccgcaa tcagcagaag ttcctcgaag aacagaacaa gttgatcgac cgttttcggg
      541 ccaaggcttc caaagccagc atggcgcaat cgcgtatcaa gatgctcgaa cggatggaga
      601 aggtggaggc ggtggattcc gaagctccca ccatccggat ccgtttcgat gccgcccgac
      661 agccgggtaa gatcatttcg catttgatca tccaggacaa gcgcttcggc gataatgtcc
      721 tcctgcacga taccgaggcc atgatccgcc ggggcgataa gatcgcgctg atcggtgcga
      781 acgggaaagg gaagtccacg ctcctgcgga tgatcaatgg cagtgaacct tacgacggga
      841 aagccgagac ggtctataat gtagatgccg ccttttatgc gcagcatcag ttggagtctt
      901 tgcacctgca aaacgacctg ttgaccgagc tgcagtcgtt cgctccggag aagaaggaat
      961 ccgaattgcg gggcatcctt ggctcgttcc tcttttcggg tgacgatgtg ttcaaaaaga
     1021 tcagcgtatt gtcgggtgga gagaaggcga ggatcgcgtt agccaaggtg ttgttgtcaa
     1081 aggcgaactt tctcctgctc gacgaaccga ccaaccacct cgacatggcg acggtttcca
     1141 tgctggtgga tgtcttgaac gattacgaag ggtcgttcct cgtcgtttcc cacgatcgcc
     1201 atttcctcag ttgcgtagcc aacaggatct ggtggatcga ggaccagcat ttgcgtacct
     1261 acgaggggtc ctacgaggag tacgaagagt ggaagacgcg gcagttgaag gccgaagcgg
     1321 ttgcggaaaa aaagcagccg gttcagcaaa aggtcgaaaa accgaagctg gagcggagtg
     1381 acgaggaaaa gaagcagcgt cagcgaacac agaagaaatt cgacaaactg gaagaggagc
     1441 tcgaacaact ccggcagtcg cgtcacgaac tggagaacct tttggcccgc cccgaagtat
     1501 acagcgatcc cggcaagttc caggatcacc tcgaccgttt taaccgtatc gacgccgatt
     1561 ttcaggcaaa gacgcgggag tgggaggaac tcttcgagca gttgtccggg atggaataaa
     1621 tcctaacttg cgtgaacctg cgcatggttt tccaacaccg atcccgaact gccgactccg
     1681 gcagggatcc tcgacgaagc cgaatcctgc gttttacgcg gctttcggcc ttcctggagg
     1741 ctttcctgtt tctgtgtatc ctgtttttcc tgctctgcca gttcgggtca ttctgaggcc
     1801 attcagccct ttttttatcg gatgacgcta gtccattgcg tcccgtcatt cacccgttct
     1861 ccggcgacgt tggtcaaata catttccgga tttacatgct gatttttaca tttggtttgc
     1921 ttgccaaggc ggaccattat gatcacacaa gtaacctccg gaatcaaagt cagtgtggag
     1981 accttctacc ggccggatca gagcaatccc ttgctcaacc agtacgtttt cgcttaccgg
     2041 attacgatca ccaacaacag tgatttttcg gtgcagctcc ggcgccgcca ttggttcatc
     2101 atcgactcca gcgccaccaa ccgcgaagtg gaaggcgagg gcgtggtcgg cgaactgccg
     2161 atcctcggga cgggtgaatc ctatcagtac gtgtcgggtt gcaacctcga ctcggaactc
     2221 ggcaagatgt acggtacgta tctgatggaa cggttgatcg acaagaagca attctatgtc
     2281 cgcatccccg aattccagat gatcgtaccg tacaagctca actgaaattc ctgatcaaca
     2341 acacgccatg ggctgctccg aaagggcggc cttttttcgt catgtccgga tgatcttcgg
     2401 caacttctta cagccg
//
LOCUS       DAOTON010000430         2413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_34181_length_2413_cov_3.914334, whole genome shotgun sequence.
ACCESSION   DAOTON010000430 DAOTON010000000
VERSION     DAOTON010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34181_length_2413_cov_3.914334"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="P5292_08710"
     CDS             <1..243
                     /locus_tag="P5292_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39246.1"
                     /translation="FYLQPNPARDRVEVVFTASEDGRNHIRVTNYNGQIVYDRETDVH
                     AGVNRHPVDIGSLPSGIYFVTLENEVEILQMRLVKD"
     gene            582..>2413
                     /locus_tag="P5292_08715"
     CDS             582..>2413
                     /locus_tag="P5292_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRS39247.1"
                     /translation="MLRLLPRFAFFYLALFGCFRMLFLWSNRPVQAPFSSREEALSFW
                     YGLRMDLSTLAYLSVVPVLLWLLLQRRESKALQNIHKLYQRLVLIILVLIGLGNVAVY
                     HFWGTLLNYRAISYLSDPKEAFVSLSGGQAFLLVLVIAAFLSLGWFGQNALRMVKLPI
                     SGNRRSIIWQLPLLLAGIVLGIRGGWQQLPMNESLVAYSRHHFLNNAAVNPAWHLSYE
                     IRTAGIGSSNPFVVMPEAEAEAAVRELFAERDDSVTRVLGLNRPNIVLIQLESFTADV
                     VGAMGGEPGISPNLDTLIAQGLLFDSIYSSGFRTDQGITSLLNGWPATPYHSVMRSEE
                     KSSRLPSLTKSLAAQGYRTECYYGGASNFSNLNVYLAGQGFDRILEQDSFPSSVERGR
                     WGVPDGPLLEHCAAEAGAGSAPFFAFIITLSNHEPFDVPGAKRYPGENDADRFRNAAA
                     YTDSVLGDFFRAARKQPWYDSTLFVLVADHAHELPKGRDIVFPIGRRIPLVLFGTPVL
                     ERYRGQRVHAVGGHHDLPRTLLAQLDLDSRSFDWSKDLLSPGVRDFAYLPAEDFVAWV
                     TRDDWFIWSRKKNAIVEAARDDLKRDRAKELRAAKAFSQVHYGR"
BASE COUNT          468 a          725 c          637 g          583 t
ORIGIN      
        1 ttctacctgc aaccgaatcc cgcccgcgat cgtgtggaag tggtttttac tgccagcgag
       61 gatggccgca accatatccg ggttacgaat tacaacggac aaatcgttta cgaccgtgag
      121 acggacgtgc acgcgggcgt taaccgccat cccgtcgata tcggttccct gccttccggc
      181 atatacttcg ttacacttga aaacgaggta gagatcctgc agatgcgact cgtgaaggac
      241 tgagtaaggt cgctgcacgt gcctgaatcg atccgcgaat cgtaattgct gctatcgatc
      301 tcctgtacgt ttcccccgtc gatactgatt tcaatcccaa tcccaatggg cgttcaatcg
      361 aacgcccatt gggattagat catgaattgg tggatatcgt cggggaatgg tccgctaaga
      421 gtgtgcactc gtccacccgg cttgtcacac ttcggctgcg attgatcctg ttgtttctcc
      481 gatttacgct tatccccgac atttccctcg ctagtcgtat tttgtggcga attgatgatg
      541 cgcatcggcc tgaaccgaga acatcacgat aacgcttctc cgtgctacgc ttgctccccc
      601 gtttcgcttt tttctacctg gcactcttcg gctgtttccg catgctgttc ttgtggagca
      661 atcgaccagt acaagctcca tttagttccc gtgaggaagc gttgtccttt tggtacggat
      721 tgcgcatgga tctctccacg ctcgcgtact tatcggtagt gccggtcttg ctctggcttt
      781 tgttgcagcg acgcgagagc aaagcgctac aaaatatcca taagctttat cagcgactgg
      841 tcctgatcat cttggtgctg atcggactcg gcaatgtggc tgtttaccat ttctggggaa
      901 cgctgctcaa ctatcgcgcg atctcgtacc tctcggatcc gaaggaggct ttcgtatccc
      961 tctcgggcgg acaagcattc ttgttagtgt tggtgattgc tgcctttctc tccttgggtt
     1021 ggttcggtca gaatgctttg cgcatggtga aactgccaat cagtggaaat cgacggtcca
     1081 tcatttggca actgccgctt ttgcttgcag gcatcgtcct tggcattcgc ggcggctggc
     1141 agcaattgcc gatgaacgaa agcctcgtcg cttattcacg acaccacttc ctcaacaacg
     1201 ccgccgtcaa ccccgcctgg cacctcagct acgagatccg tacggccggc atcggcagca
     1261 gcaatccgtt cgtcgtgatg ccggaggccg aagcggaagc cgccgttcgc gaactcttcg
     1321 cggaacgcga tgattccgtc acccgggtgc tcggactgaa tcgtccaaac attgtcctca
     1381 tccaactcga aagcttcacc gccgatgtcg tcggcgcgat gggcggagag cctggcatca
     1441 gcccgaatct tgacacgctc atcgcgcagg gcttgctttt cgattcgatc tactcttccg
     1501 gcttccggac ggatcagggc atcacctcgc tactcaacgg ctggccggca acaccgtatc
     1561 attcggtcat gcgcagcgaa gaaaaatcat cccgccttcc ttcgcttacg aaatccctcg
     1621 ccgcgcaggg ttatcgtacc gaatgctatt acggcggcgc cagcaatttc tccaacctga
     1681 atgtctacct cgccggacag ggcttcgacc gtatcctgga acaggatagc tttccttctt
     1741 ccgttgaacg tggccgctgg ggtgttcccg acggaccttt actcgagcat tgcgcagccg
     1801 aggctggtgc cggcagtgcg ccgttcttcg cgtttatcat caccctgagc aaccacgagc
     1861 cgttcgatgt acccggagcg aagcgttacc ccggcgagaa cgacgcagat cgttttcgca
     1921 acgccgctgc ttacacggat tccgtactgg gtgacttctt ccgtgctgcg cgaaagcagc
     1981 cctggtacga cagcacgttg tttgtcctgg tggccgatca cgcacacgaa ctgccgaaag
     2041 gacgcgacat cgtctttccc atcggccgca ggatcccatt ggtacttttc ggaacacccg
     2101 tgctggaacg ctaccgcgga caacgtgtac atgcagttgg tgggcatcat gatctgccca
     2161 ggacgctgct ggctcaactc gacctcgaca gccgctcgtt cgactggagc aaggacctgc
     2221 tatcgcccgg cgtgcgggac ttcgcgtatt tgcccgccga ggacttcgta gcctgggtca
     2281 cacgtgacga ctggttcatc tggtcacgca agaagaacgc gatcgtcgaa gccgcccgcg
     2341 acgacctaaa acgcgatcgc gccaaagaac tccgcgctgc caaagcgttt tcgcaggtgc
     2401 actatgggcg gtt
//
LOCUS       DAOTON010000431         2399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_34467_length_2399_cov_2.414676, whole genome shotgun sequence.
ACCESSION   DAOTON010000431 DAOTON010000000
VERSION     DAOTON010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34467_length_2399_cov_2.414676"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="P5292_08720"
     CDS             <1..1152
                     /locus_tag="P5292_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39248.1"
                     /translation="ANSGYAVYGKNDDWWEAIGGYFIGGYEGVAGIVAPTGSQYYHGV
                     YGEAYGSNAPKIGVLGNSDMTGVYGSTFGPGRGQTTYYGPNYDETIGVTGDAQTMNAT
                     NGASYGIYGKAGNPNSVLNAGMFGYARSATGGFSARNYGVLGVSEGPSNATGVIGIAD
                     PGASSFGGYGIEGDVLSGTDQVGVYGYCAGATATDYAGYFQGNLYATSASSGIKAFKI
                     DHPQDPANKYLYHSSVESNEMVNIYRGNVVTDANGRATVDLPAYFETLNKDVTYQLTV
                     VNEFAQAIIGEKVYDNRFVIRTDKPNVEVSWMVTGVRKDPLAEKYRIRDVVEKPASEK
                     GTYLTPELYGKTINEATRKPRYAERKAGNNTLIDFREDPRLKARRNGAK"
     gene            complement(1158..1676)
                     /locus_tag="P5292_08725"
     CDS             complement(1158..1676)
                     /locus_tag="P5292_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39249.1"
                     /translation="MCRIRLDAYAQFAGGQGLWSCGILATPNERIGLSIGFGHTPPAY
                     GNIFTLTGKCVYTPWNIRLNDAFTLYPLGVGLYCSLSSSENIKWAWDKTKYPNNYYWW
                     PASLRTGPIVQFALQGRFGSYLITPYFEVSTNDLYLASYFGNARSLSLPDILVMGAGV
                     RFRLKPSRPGSH"
     gene            complement(1717..>2399)
                     /locus_tag="P5292_08730"
     CDS             complement(1717..>2399)
                     /locus_tag="P5292_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRS39250.1"
                     /translation="ASTPGDTIVLVFSGDQQRFYYQVDRMVDDINDLQQVDAVFLVGD
                     LTDFGLIREFGWLNESLLRLQCPFIAVIGNHDCLANGTELFERTYGPLNQHFDWAGIR
                     FVLHNTNSREFSFNGQVPDINWMRSVVEDTSAYRASVFVSHIKPFDIGDFDAALEDDY
                     VQVLRSARSPLFSVHGHDHISNSVTPYGSDLHFHVAGSPSYSSYALVKIFTLDDGTIS
                     YTLSRRTF"
BASE COUNT          623 a          657 c          651 g          468 t
ORIGIN      
        1 gccaacagcg gctatgccgt gtatggcaag aacgacgact ggtgggaagc catcggcgga
       61 tattttatcg gcggatacga aggagtagcc ggcatcgtcg cgcctaccgg atcacagtat
      121 tatcacgggg tatatggtga agcgtatgga agcaatgcgc ccaagatcgg tgtcttggga
      181 aactccgaca tgaccggtgt atacggcagc acgttcggac ccggcagagg ccagaccacc
      241 tattacggcc cgaactatga cgaaaccatc ggtgttaccg gcgatgcgca aacgatgaat
      301 gcgaccaacg gcgccagtta cggcatctac ggaaaagccg gaaatcccaa ctcggtcctc
      361 aatgccggca tgttcggcta cgcacgatcg gctaccggtg gcttttccgc gcggaattac
      421 ggagtgctcg gggtatcgga agggccaagc aacgccacgg gagtgatcgg tattgccgac
      481 ccgggcgctt caagttttgg cggatacggc atcgaaggtg atgtcctatc aggcaccgac
      541 caggtcggtg tttacggtta ttgcgctggc gctaccgcga ccgattacgc cggctatttc
      601 cagggaaacc tgtatgcgac ctccgccagt tcgggtatca aagccttcaa gatcgaccac
      661 ccacaggatc ccgccaacaa gtacctctat cattcgtccg tcgagagcaa cgagatggtg
      721 aatatctacc ggggcaatgt tgtcaccgat gccaatggcc gcgcgaccgt ggacctgccg
      781 gcttatttcg aaacactcaa caaggacgtg acctatcagc tcacggtagt gaacgaattc
      841 gcccaggcca tcatcggcga aaaagtgtac gacaaccgct tcgtcatccg caccgacaaa
      901 cccaatgtgg aagtgagttg gatggtcacc ggtgtgcgca aggatccact ggcagagaag
      961 taccgcatcc gggatgtggt cgaaaaaccc gcttccgaga aaggaaccta cctcacaccc
     1021 gagctatacg gcaagacgat caacgaagct acccgcaaac cacgttacgc ggagcggaaa
     1081 gccggcaaca acacactcat cgacttccgc gaagacccga ggctgaaggc ccggaggaac
     1141 ggcgccaagt gatggagtta gtgcgatccg ggcctcgagg gcttcaaccg gaaacgtaca
     1201 cctgctccca tcaccagtat atccggaaga ctcaatgatc gggcattccc aaaatagctt
     1261 gccaggtaga gatcattggt ggaaacttcg aagtagggag tgatcagata tgatccaaac
     1321 cgcccttgaa gtgcgaactg tacgatcggg ccggtacgca gcgaagcggg ccaccagtaa
     1381 tagttgttcg gatacttcgt tttgtcccag gcccacttga tattctcgga gctgctcagc
     1441 gagcaataca atcctacacc cagcgggtag agtgtgaaag catcgttcag ccggatgttc
     1501 cacggggtgt acacgcattt acctgtcagc gtaaaaatat tcccgtacgc cggtggtgta
     1561 tggccgaagc cgattgaaag gccgatccgc tcgtttggag ttgccaggat tccgcaggac
     1621 cacagtcctt gcccgccggc gaattgagca tacgcatcca accgaatcct acacatgctc
     1681 ttggccgtgt cggatgggtc cgcgaaagcc cgaacactaa aaagtacgac gagaaagagt
     1741 ataactgatc gtcccatcat cgagtgtaaa gatcttcaca agggcatagc tgctatagga
     1801 cggtgatccg gcaacatgaa aatgcaagtc actgccatat ggcgtaacag aattggaaat
     1861 atgatcatgt ccatgtacac tgaataatgg tgagcgggcg ctgcgcagca cctggacata
     1921 gtcgtcttcc aatgcagcgt cgaaatcgcc gatatcaaag ggcttgatat gggaaacaaa
     1981 aacactggct cgataggcgg atgtatcctc gacaacgctg cgcatccagt tgatgtcagg
     2041 cacctgtcca ttgaatgaaa actcccggct gttggtgttg tgcagaacaa atcggatccc
     2101 ggcccagtcg aaatgttgat tcaaaggccc ataagttcgc tcgaacagct ccgtaccatt
     2161 ggcaagacaa tcatgatttc cgatcacggc gatgaacgga cactgcaacc gaagcaagga
     2221 ttcattcagc cagccgaact cccgaatgag cccgaagtcg gtcagatcgc caaccagaaa
     2281 gacggcatct acctgttgca ggtcattgat gtcgtccacc atccggtcaa cctggtaata
     2341 aaaacgctgc tggtctccgc tgaataccag cacgatcgta tcacccggtg tactggcct
//
LOCUS       DAOTON010000432         2396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_34513_length_2396_cov_3.064075, whole genome shotgun sequence.
ACCESSION   DAOTON010000432 DAOTON010000000
VERSION     DAOTON010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34513_length_2396_cov_3.064075"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="P5292_08735"
     CDS             complement(<1..765)
                     /locus_tag="P5292_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS39251.1"
                     /translation="MAQTNPTISKGALPTLITVFFFWGFVAASNGIFIPFCKTHFQLN
                     QFQSQLIDTSFYGAYFFGSLLLYLMSGVSGVDILNRIGYKNGIIIGLSMSVIGALSLA
                     FVSVSASATFAMVLASFFIIALGFSLQQTAAQPFTIALGSPETGAHRLNLAGGINSLG
                     TLLGPLAVSYMLFGDLQNGGTATIQSIQTLYLTLAGLFLFVALFFLFSKNLPTIHQEE
                     HVEKSSKATNILFLISIPTVLILFFNDGGVQVSRPVR"
     gene            complement(862..1707)
                     /locus_tag="P5292_08740"
     CDS             complement(862..1707)
                     /locus_tag="P5292_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HRS39252.1"
                     /translation="MLLIADGGSTKADWAVLEDNGQVRLLSSEGFNPNQHDAQQIALK
                     LAGDPALMALAEQVSSVRYFGSGCSSPELKAIAGAGLRAFFKHASIEVDHDVQAAALA
                     VCGNTPGIACILGTGSNACYFDGRHAQPARSGLGYILGDEGSGTWFGKRLVTRYLYGL
                     MPDDLSREFDKHYALTRDEVIRRVYRESGANNWLASFAPFLSMHREHPWILETLDAGF
                     AEFFELCVRPIQVPTPLPVHFVGSIAHAFQPELIRTGTRLGQPVGTILQKPIEGLVRH
                     FRQLH"
     gene            complement(2032..>2396)
                     /locus_tag="P5292_08745"
     CDS             complement(2032..>2396)
                     /locus_tag="P5292_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS39253.1"
                     /translation="LSRIHPDYSLDAFLRVKARYLASWLQQHPPGDRPLVVCGGSRNI
                     RNKLEVLQGEGIRFDAYTDVVNRPGHPLRYIPVSSLPAPGEAFFINLIGQRGVRTAIR
                     QLLMEQGHQEEQDFILLA"
BASE COUNT          559 a          635 c          720 g          482 t
ORIGIN      
        1 cctgacgggt cgcgatacct gcacaccgcc gtcgttgaag aacaagatga gcaccgtcgg
       61 aatggagatg agaaaaagga tattcgtcgc cttcgaactc ttttccacgt gctcttcctg
      121 gtgaatggtg gggaggttct tcgagaacag gaagaacaag gccacgaaga gaaagagtcc
      181 tgccagggtg aggtacaggg tttggatcga ctgaatcgtc gcggtgccgc cgttttgcaa
      241 atcgccgaac agcatgtaac tcaccgccag cggaccgagc aatgtgccca gcgagttgat
      301 gccgccagcc aggttgagcc ggtgcgcgcc ggtctccgga cttcccagcg cgatggtgaa
      361 cggttgagcg gctgtctgct gcagtgaaaa gcccagcgcg atgatgaaga acgaagcgag
      421 caccatcgcg aacgtggccg aagccgatac cgagacgaac gccagactaa gcgcgccgat
      481 caccgacatc gacaatccga tgatgatccc gttcttgtag ccgattcggt tgaggatgtc
      541 gacacctgaa actccggaca tcagatataa cagcagggag ccgaagaagt aggcgccgta
      601 gaaggaggtg tcgatcagtt gcgactgaaa ctgattcaac tggaaatgtg tcttgcagaa
      661 cgggatgaag atgccgttcg aagcggctac aaagccccag aagaagaaga cggtgatgag
      721 cgtcggtaat gcgcccttgc tgatcgtcgg attcgtttgc gccatgccgg gaagtgttat
      781 gctgtcaaat acggtaaaat cgtacttgga tacgcaaggt cctgatgaaa gttattcgcc
      841 cgactgtgta cagctatgcg gttaatgaag ctgacggaag tgtcgaacca gtccttcaat
      901 gggtttttga aggatcgtac cgaccggctg ccccagccgc gtaccggtcc ggatcagttc
      961 cggttggaag gcgtgggcga tgctgccgac gaaatgaacc ggcagcggtg tcgggacttg
     1021 gatcggacgt acgcagagtt cgaagaattc agcgaatccc gcatccagcg tttcgagtat
     1081 ccagggatgt tcccgatgca tgctcaggaa gggtgcgaag gaagcgagcc agttgttcgc
     1141 tccgctttcg cggtacacac gacgaatgac ttcatcgcgg gtcaaggcgt agtgtttgtc
     1201 gaattcccgg ctcaggtcgt ccggcatcaa tccgtacaga tagcgggtga ccagtcgctt
     1261 cccgaaccag gtgccgctgc cttcgtcgcc caggatatag ccgagtcccg aacgcgccgg
     1321 ctgagcgtgc ctgccatcga agtaacaggc gttgctgccg gttcccagga tgcaggcaat
     1381 gcccggtgtg tttccgcaaa ccgccagggc tgcggcctgg acatcatggt ccacttcaat
     1441 ggaagcgtgt ttgaagaagg cgcgaagtcc ggcgcctgcg atggctttca gttccggcga
     1501 agaacaaccc gaaccgaagt accggacact gctgacctgt tccgccagcg ccatcagtgc
     1561 cgggtcgcct gccagtttca acgcgatctg ttgcgcatca tgctggttcg gattgaatcc
     1621 ttccgagctc aacagccgta cctggccatt gtcttcaagg acggcccagt cggctttggt
     1681 cgatccgccg tcggcgatca ataacatgct gtttcctgca ttggttcgta gttgcaatca
     1741 tacaaaaacg aagcgggggg aaaggagcga tcgaaatgaa tctgtaaaca gggctgggat
     1801 aaaatggacc gccggcctgc gtccacacag cgcagtccgg cggcggcatt gaaaaccgga
     1861 aacacacatc actaaaactc ctgtttggcg tcgccagggg tcacctgaca acgtggagtg
     1921 agccggacaa tgttgcacta aagtatggtc gggatgtgcc ttgagacgaa tgaaccatgc
     1981 cggtcaggta tttgggtctt ctgaatggga caaaaccgga gtcgaccggg atcaggcgag
     2041 caaaataaag tcttgttcct cctgatgtcc ctgttccatt agaagttgcc ggatcgctgt
     2101 ccgaactccc cgttgcccga tcaaattgat gaaaaaggcc tctcctggcg ccggaaggga
     2161 cgagaccggg atatagcgga gcggatgccc cgggcgattc actacatcgg tataggcgtc
     2221 gaagcgaatg ccttcacctt gcaagacctc cagcttattc ctgatgttcc ggcttccgcc
     2281 acagacgacc aagggtcgat cacccggagg atgttgctgc agccagcttg ccaggtagcg
     2341 ggctttgaca cggaggaagg catccaggga gtaatccgga tgaatcctgc tcaatc
//
LOCUS       DAOTON010000433         2395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_34530_length_2395_cov_3.355128, whole genome shotgun sequence.
ACCESSION   DAOTON010000433 DAOTON010000000
VERSION     DAOTON010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34530_length_2395_cov_3.355128"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /gene="rnpB"
                     /locus_tag="P5292_08750"
     ncRNA           complement(<1..351)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5292_08750"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            499..>2395
                     /locus_tag="P5292_08755"
     CDS             499..>2395
                     /locus_tag="P5292_08755"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS39254.1"
                     /translation="MRFILFCFIFLFSIEPMVQAQTPGNHTVSGFIREAATGESLLGA
                     NVYIKENLKGVTTNQYGFYSITLPAGQYTLVISYLGFKSEERSVDLNKDLRINISLQS
                     GAIETKEVTISGEKADRNVQSTDMGKEKIEIEKIKTLPAFMGEVDVLKTIQLLPGVSS
                     AGEGNSGFYVRGGGPDQNLILLDEAVVYNASHLFGFFSVFNADAVQNVTLTKGGMPAN
                     YGGRLASVLDIQMKEGNNQKFGAEGGIGFVSSRLTVQGPIRKDTGSFIVSARRTFIDL
                     FMGKPFVSSSSDIAGNKYYFYDLNAKVNYRLGENDRLFLSGYFGRDVFNFKSQESDFY
                     IKVPWGNATATARWNHLFTDRLFMNTSLIYTNYKFEFEGGQDAFNFKLFSGITDYNLK
                     TDFTWLPNVLHTVKFGAQYIFHVFVPSNASAQSGDVKFDLGKVLRNYAHDAAIYVNDE
                     YEIDDRWKVNGGLRLSRFQQVGPFDRYVRDPVRNVVTDTLHYSPGENIKTYLKLEPRL
                     SVRFALNDRSSIKAAYTQNYQYIHLASLSSISLPTDVWIPSTDIVRPQFGTQYAIGYF
                     RNFKDNAWETSVEAYYKEMKNQVEYREGFLPEETVNDNIDNNFVFGRGWSYGTEWFIK
                     KSAGKFSG"
BASE COUNT          584 a          654 c          591 g          566 t
ORIGIN      
        1 gcaagcggga cccttgtcat ttatctgggc gtgcattcac atgcccgctc gatcgaccta
       61 cccgccgtgg cgcaaaagca tcggatgggc caccctcccg gaaacgagtt ccttccacgg
      121 cttatttggt ctttcaatgc ctgaggttta cccgcagtac cgtcgccggt agctgccgtg
      181 agctcttacc tcacgttttc acccttaccc ggaacttgcg ctcctggcgg tttagtttct
      241 gtggcacttt ctatgatccc tttcaggatc ccttcccgtt aggaagcagg ctgcccgata
      301 ttgcccggac tttcctcccc gactgaacgg agcgacaagg tcaccggcga tgataagaac
      361 gtgttgcaaa gatacatcgc aaatcggggg cattcaagtt gacacgaaga atcactgatt
      421 tttaagaaac cggtaaccaa ttatccaatg gtctgtttct accttcgcgg cgccaatccg
      481 gccttcctta tctgctttat gcgttttatt ctgttttgct tcatcttcct gttctccatc
      541 gaaccgatgg ttcaggctca aactccggga aatcataccg tcagcggctt cattcgggaa
      601 gcagctaccg gcgaatcact cctcggagcg aatgtctaca tcaaagagaa cctgaaaggt
      661 gtaacgacca accagtatgg tttttattcc atcacgcttc ctgccgggca atacacgctg
      721 gtcatctcct acctgggttt caagagtgag gagcggtccg ttgacctgaa taaggaccta
      781 cgcatcaaca tctccctgca atcgggggcg atcgagacca aggaagtcac gatcagcggc
      841 gaaaaagctg accggaatgt ccagtcaacc gacatgggta aggagaagat cgagatcgaa
      901 aaaatcaaga cccttcccgc attcatgggc gaagtcgatg tcctgaagac gatccaacta
      961 ctacccggcg tttcatccgc cggagaagga aactccggtt tctacgtacg cggaggtggc
     1021 cctgatcaga acctcattct cctggatgaa gcggtcgttt acaacgcttc tcacctgttt
     1081 ggtttcttca gtgtattcaa tgctgacgcg gtgcagaatg tcacccttac caagggaggc
     1141 atgcccgcca actacggtgg tcgactggca tcggtgctcg acattcaaat gaaggaaggg
     1201 aacaaccaga aattcggagc cgagggtggt atcggtttcg tatccagccg cctgacggtt
     1261 caggggccga tccggaaaga taccgggtcg ttcatcgtgt cggctcgccg cacgttcatc
     1321 gacttgttca tgggaaaacc gtttgtgagc agcagctccg acattgccgg caacaagtat
     1381 tacttctacg acctcaacgc aaaggtcaac taccgtcttg gtgaaaatga tcgtttgttt
     1441 ctgagcggct atttcggtcg cgatgttttc aacttcaaaa gccaggaatc ggatttctat
     1501 atcaaagttc cctggggtaa tgccacggcc acagcgcgtt ggaatcactt gttcaccgac
     1561 cgcctgttca tgaacacctc gctcatttac acgaattaca agttcgaatt cgaaggcgga
     1621 caggatgcct tcaacttcaa gttattcagt ggcatcaccg actacaacct aaagaccgat
     1681 ttcacctggc taccgaacgt cttgcatacg gtcaagtttg gtgctcagta tatcttccat
     1741 gttttcgtac caagcaacgc ttccgcgcaa tcaggagatg tcaaattcga tctggggaaa
     1801 gtgctgcgca attatgccca cgatgcagcg atatacgtca acgacgaata tgaaatcgac
     1861 gaccgttgga aggtcaacgg cggacttcga ctttcccgct tccagcaagt cgggccgttc
     1921 gaccggtatg tacgagatcc ggtccggaac gtagtgaccg acaccttgca ctactcgccg
     1981 ggagaaaaca tcaagaccta tctgaagctc gagccccggc tttcggtacg atttgccttg
     2041 aacgaccgct cctcgatcaa agcggcctat acgcagaatt accagtacat ccacctggct
     2101 tcgctctcct cgatctcgct gcctacggac gtctggattc cttcgacgga tatcgtccgc
     2161 ccgcaattcg gaacacaata cgcgatcgga tacttccgga atttcaagga caatgcctgg
     2221 gaaacctcgg tggaggcgta ttacaaggag atgaaaaacc aggtcgaata ccgcgaagga
     2281 ttcctgccgg aagaaacggt gaacgacaac atcgacaata acttcgtatt cggaagggga
     2341 tggagttacg ggaccgaatg gttcattaaa aaatcagccg gaaagttcag tggct
//
LOCUS       DAOTON010000434         2395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_34542_length_2395_cov_2.393590, whole genome shotgun sequence.
ACCESSION   DAOTON010000434 DAOTON010000000
VERSION     DAOTON010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34542_length_2395_cov_2.393590"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="P5292_08760"
     CDS             <1..561
                     /locus_tag="P5292_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRS39255.1"
                     /translation="INRMLTVDMMLKRAMKGELDLMGPATAVANELMSIPDFSGGEEQ
                     LFDAEKKLLANFKKAVLMVAGSAVQKLMMELAKEQEVLMNIADMMIDVYIGESLQLRV
                     EKMVSLRGEAACAEAIDILRVWLYDASDRINKAGKDAIGSFSEGDEQRLLLMGLKRFT
                     KTSPLNVKEARRRIAAKLLSENKYCF"
     gene            737..>2395
                     /locus_tag="P5292_08765"
     CDS             737..>2395
                     /locus_tag="P5292_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39256.1"
                     /translation="MGLLAASVAKAGSFFERYYGSGTPDQGVHFTQGIDGSFLIVGSS
                     WDSLLAANRYYLLHLDSTGTPLFDTTFSTSFQTLAQSVTALPNGGFAMVGTRIGFFYD
                     AVTEVHLLDASARPLNAQTFPPFDGWGTSGVGIFTARDSSICVTHFTDGFLSSNFFSM
                     LNLEDDLGVRWTAFLAYDGGAFNEHSAVQCEDGTFLSTAYFQDYWYSWPDILYATEWR
                     TINENGSQRTDSIYSFGMESRSIACGPGKRTLLSGRTDSTGNGDGCTVLLDSVGMQLL
                     RRTWGSDAYENPVQIIALPDSGWAILAETASLQFPGRQDLLLCRFNASGDSLWSRRFG
                     GPANDLGRQLMSLPDGGFAFLGTYEDAGVGRTYFVRTDANGIVQTPYRITGDTSFACA
                     GDTIQLAIQPSPPTGAHIEWSNGDTTESVQIVVSGIFQATVYVSGSDPVMTNSFPVYI
                     APLPSADLGLQDTVALCRKTPLELSGTTDPTYTYAWYRNDTLIDGETAPSFTPEFSGT
                     YTLIVSNYCSLDTDRVVIDSLYELPAIPLVTASRANPICPGDSVQ"
BASE COUNT          508 a          621 c          645 g          621 t
ORIGIN      
        1 atcaaccgga tgctgacggt cgatatgatg ctcaagcgcg ccatgaaagg cgagttggac
       61 ctgatgggtc ccgcaaccgc agtggcgaat gaactaatgt cgatccccga tttcagtggt
      121 ggcgaagaac agctcttcga cgcggaaaag aagttgctgg cgaatttcaa aaaggccgtc
      181 ctgatggtgg ctggctccgc tgttcagaag ctgatgatgg aactggccaa ggagcaggaa
      241 gtgctgatga acatcgccga tatgatgatc gacgtgtata tcggagaatc gcttcaattg
      301 cgggtggaga aaatggtctc cctccgtggc gaagcggcct gtgccgaagc gatcgacatc
      361 ttgcgggtct ggttgtacga tgcatcggat cgcatcaaca aagccggcaa ggatgccatt
      421 ggttccttct ccgaaggtga tgagcagcga ttattgttaa tgggtttgaa gcgatttacc
      481 aaaacttcgc ccttgaatgt gaaggaagcc cgacgccgta ttgccgcgaa attgctctcg
      541 gaaaacaagt attgcttcta aacgaagttc ttgtgcgata aatgaagggc ccttttacgg
      601 gccctttttg ttgatatccg gtagagaatt catggaatct tcctgatggc ttaatgttca
      661 ggctcgcaat tagttgctaa tttcgcccgt atccgcgtaa cgagtgaatt cttttacccg
      721 attcatcatt ttgttcttgg gcctgcttgc ggcttctgtc gctaaggccg gttcattttt
      781 tgaacgctac tatggctcgg gaactccgga tcagggggtt cattttacac aggggatcga
      841 cggtagtttt ctgattgtag gtagttcctg ggattcactc ctggccgcca accggtacta
      901 cttgttgcat cttgactcca ccgggacgcc tttattcgac acaaccttct ccacctcctt
      961 tcaaaccctg gcgcaatcgg tgacggcctt gcccaacggt ggatttgcca tggtaggtac
     1021 acgcatcgga tttttctacg atgcggtaac cgaggttcat ctgttggacg cttcggcacg
     1081 ccccctgaat gcccagacgt tcccgccgtt cgacggttgg ggaacatccg gcgtgggaat
     1141 attcaccgcc cgcgacagca gtatttgcgt gacgcatttc acagatggtt ttttgtcctc
     1201 caatttcttt tccatgctca acttggaaga tgatctcggt gtacgctgga ccgcctttct
     1261 tgcatacgat ggaggtgcat tcaacgaaca ttccgcggtg cagtgtgagg atggaacttt
     1321 tttatcgact gcttatttcc aggattactg gtattcgtgg ccggatattc tctatgctac
     1381 tgaatggcgc acgatcaacg agaacggatc gcaacgaacg gattccatct actcctttgg
     1441 aatggagagc cgatcaattg cctgcggacc cgggaagagg accctgcttt ccggaagaac
     1501 cgactccacc ggtaatggcg atggatgcac cgttctgctc gattccgttg gcatgcagtt
     1561 actgcgtcgt acttggggaa gtgacgcgta tgaaaaccct gttcagatca ttgctcttcc
     1621 tgatagtggc tgggcgatcc tcgcggaaac cgcttctctt caattcccgg gccggcagga
     1681 tcttttactc tgcaggttca atgcgtctgg ggatagtctt tggtcgcgga ggttcggtgg
     1741 tccggcgaat gaccttggcc gacaactgat gagccttccc gatgggggat ttgcctttct
     1801 gggtacctat gaagacgctg gagttggtcg aacctatttt gtccgtaccg atgccaacgg
     1861 aattgtccag actccctatc ggatcacagg cgatacttcc ttcgcctgcg caggcgatac
     1921 catccaactg gccattcaac catcaccgcc gacgggggca cacatagagt ggtcgaacgg
     1981 cgatactacc gaatctgtac agatcgtggt gtcgggtatt ttccaggcta ccgtctacgt
     2041 gtccggtagt gatccggtca tgaccaatag ctttccggtc tacattgcac cattgccttc
     2101 tgccgatctg ggtttgcagg ataccgtcgc cttgtgtcgg aaaacgccat tggaactatc
     2161 cggaaccacc gatccgacct atacttacgc atggtaccgg aacgataccc tcatcgatgg
     2221 cgagaccgcg ccatcgttta cgccggaatt cagcgggacg tacaccctta tcgtcagcaa
     2281 ttattgtagc ttggatacgg atcgcgtcgt tatcgactcg ctctacgaac taccggccat
     2341 accgttggtt accgcgagcc gggccaatcc gatctgtccg ggcgacagtg tccag
//
LOCUS       DAOTON010000435         2387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_34694_length_2387_cov_4.078045, whole genome shotgun sequence.
ACCESSION   DAOTON010000435 DAOTON010000000
VERSION     DAOTON010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34694_length_2387_cov_4.078045"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2082
                     /locus_tag="P5292_08770"
     CDS             <1..2082
                     /locus_tag="P5292_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRS39257.1"
                     /translation="SSTWVFFTTPTPAATNQEIPAASLLINEVMASNTGTIADNYGQF
                     DDWFELYNPTADTLDLAGWFASDDPAHPTRYRFPHGTDSTKVPPYGFRIVWADGQPEQ
                     GVLHATFGLSASGDCVYLYKPDAITISDNICIGAAVANVSYGRTYDGANSWIDFNPAT
                     PGATNVQVGAERIVINEVLTVNTNSVTDNFGEREPWIELHNPNPDTVDLGGWFLFNLV
                     GNPGQFRFPFDNDSLRIPPGGFLLVYADNETRQGSLHVPFQLNGGPACIWLYKPDQAG
                     SDSICFGAIAADQSYGRQFDDDPVWINFPVPTPGATNYSPQAATALLNEVQPVNTNSA
                     ADNYGDFDPWVEIYNPNPDTLDLGGWYISDVSSQPLKHRFPLNNDSLRIPPGGLFLVW
                     SDNEVVEGAAHVNFILSNGPACVYLTKPDGLTRSDSLCYPLLAADESYGRRADGDPVQ
                     VTFTGPTPGGTNWNFTPVNVLINEVQPVNLSTIADEQGEFDAWVELYNPFPDTIDLAG
                     WYMANTATLGNPVTWYRFPFDNDSTKIPGDGFRLLWADAAPGQGALHLWFVLNGLNDC
                     VYLIKPDENVSDQICYTNVQPDHSYGRDGDGDPDWRDFSIPTPDSTNVDLSVGLPNAP
                     VAQQLLVWPNPVHDGQIFFSRSIDASLFDQTGRMLRYTEDQPSMDLQGLASGVYILLA
                     RTGERIRIVVY"
BASE COUNT          505 a          693 c          636 g          553 t
ORIGIN      
        1 tcctccacat gggtgttttt tacaacgccc acaccagccg ccaccaatca ggagatccct
       61 gccgcgagtt tattgatcaa cgaggtaatg gcttccaaca ccggaacgat tgccgacaat
      121 tacggacagt tcgacgactg gttcgaactc tacaacccaa ccgccgacac ccttgatctg
      181 gcgggttggt ttgccagcga cgatcccgcg catccgaccc ggtaccgttt cccgcacgga
      241 accgattcca ccaaagtgcc gccgtacggt ttccgtatcg tctgggccga cgggcaaccc
      301 gagcagggcg tcctgcatgc gacattcggg ctgagcgctt ccggtgattg cgtctatctt
      361 tacaaaccgg atgcgatcac catctccgat aacatttgca ttggtgctgc cgtggccaat
      421 gtgtcgtatg ggcgcacgta tgatggagcc aacagctgga tcgacttcaa tcccgccaca
      481 ccaggcgcga ccaacgtaca ggtaggagcg gagcggatcg tcatcaacga agtacttacc
      541 gtgaacacga attcggttac ggataatttc ggagaacgcg aaccctggat cgaactgcac
      601 aacccgaatc ccgatacggt tgatttgggt ggctggttcc tgttcaacct cgtcgggaat
      661 cccggacagt tccgttttcc tttcgacaac gattcgttgc gtattccgcc cggcggattt
      721 ttattggtgt acgcggacaa tgaaacccgg caaggcagct tgcatgtacc cttccagttg
      781 aatggcggcc ccgcctgtat ctggttgtac aaaccggatc aagcgggctc cgattcgatc
      841 tgtttcggcg cgatcgccgc ggaccagtcc tacggacgcc agttcgacga cgatccggta
      901 tggatcaact tcccggtgcc gacgccgggt gcaaccaatt acagtccgca agctgccacg
      961 gccttgctga atgaagttca gccggtgaac acgaattccg ctgccgataa ttacggcgac
     1021 ttcgatccct gggtggagat ctacaacccc aatccggata cgctggactt aggcggctgg
     1081 tatatcagcg atgtttcgtc gcaaccgttg aagcatcgct tcccactaaa caacgattca
     1141 ctgcgcatac ctcccggtgg attgttcctc gtatggtcgg acaatgaagt ggttgaagga
     1201 gctgcacatg tcaactttat cctgtcgaac gggccggcat gtgtctatct caccaagccc
     1261 gacggcctca cgcgttccga ttcgttgtgt tatccgttac ttgcagccga cgaatcgtat
     1321 ggtcgtcgcg cggatggtga tccggtacag gtcaccttta ccgggccgac gcccggagga
     1381 acgaactgga actttacgcc ggtcaatgta ctgatcaatg aagtgcagcc ggtcaacctg
     1441 tcgaccatcg ccgacgagca gggtgagttc gacgcctggg tcgaattgta taaccccttc
     1501 ccggacacga tcgatctggc cggatggtac atggccaaca ccgccaccct gggaaatccg
     1561 gtgacctggt accgcttccc gtttgacaac gattccacca agatccccgg cgatggtttc
     1621 cgcctgttgt gggccgatgc agcaccgggt cagggagcct tgcacctttg gttcgtgctg
     1681 aatggcctga acgattgcgt ttacctgatc aaaccggatg aaaatgtctc cgaccagatc
     1741 tgttatacca atgtacagcc cgatcattcg tatggacgtg acggcgacgg cgatccggat
     1801 tggcgcgact tctccatccc gacgccggat tctacgaatg tggacctgtc ggttggattg
     1861 ccgaatgctc cggttgcgca acagctgctg gtatggccca atcccgttca cgacggacag
     1921 atttttttca gtcggagcat cgacgccagt ctgttcgatc agaccggccg gatgcttagg
     1981 tacactgaag accagccttc catggatctt caaggtcttg cgagcggtgt ttatattctg
     2041 cttgctcgca ccggcgaacg gatacggatc gtagtatact gatccgactt ggttctgtac
     2101 tcttcggaat atccggcgca cccttgccgg agaagcaagc tgtcagccga gtgtaaaatc
     2161 acggctgtta accactccga agtgggtttc cgtcaatttc agcttggtta taaaagacat
     2221 tctttcgagg ttcgttcaag cttgaatcgt ctcccatgcg gtatttattc acgttgattc
     2281 tcctgctgat ctcgatcgct tgtctggcac agtcacgcct gcaatggacc gttagcgagc
     2341 gaaccccgac agcgcaggcc aatatgctac gcgcagcggc aaccgat
//
LOCUS       DAOTON010000436         2387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_34700_length_2387_cov_2.616638, whole genome shotgun sequence.
ACCESSION   DAOTON010000436 DAOTON010000000
VERSION     DAOTON010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34700_length_2387_cov_2.616638"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="P5292_08775"
     CDS             complement(<1..1443)
                     /locus_tag="P5292_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRS39258.1"
                     /translation="MNLLSVDRVSKAYGDKLLFQELSFGIARGEKVALIARNGTGKTT
                     LLRILAGKDVPDSGEVTYRKDVRVAYLEQEPSFDGHRTVAELIFGDENPVVQLTRQYE
                     LALEQHSRENTPASARNLEEMTHRMDAANAWDYEARIRQVLGQLDIHDLDRKILGFSG
                     GQQKRIALAKVLVEPSDLLILDEPTNHLDVEMVEWLEGYLGRANLSLLLVTHDRYFLD
                     NVTDRILELDLGKIFAYDGNYAFFLEKKAEREEAESRTQDKLRNLFRTELEWVRRMPK
                     ARGTKSKARIDAFHDLKDRMGGKRKEDGLELDVKMNRIGGKIIELKKVYKAFGDRTIL
                     KGFDYTFRTGERVGIVGKNGCGKTTFLNIISGKEPPDSGKVNIGETVVFGFYSQAGLQ
                     VKEDKRVIEVVREHADVIQLADGSKVSAGQFLQLFQFQPSLQQTYVSKLSGGERRRLH
                     LLTVLIRNPNFLILDEPTNDLDLLTLATLEE"
     gene            complement(1571..2113)
                     /locus_tag="P5292_08780"
     CDS             complement(1571..2113)
                     /locus_tag="P5292_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39259.1"
                     /translation="MKTNSLGIGSRIRHPQFGAGVIVQVKPEEYVVTFMDHGMRDLDR
                     DEQGLEIIDANDQDQDLVSLADIEVLLVNVIRKYSDIQERVELGGRWVGGKMVLQPAD
                     AALKPKEVPIETFFNKIVMLRDRLRVLEQRINAHEKLSDEDKVNIQQYITRCYGSLTT
                     FNVLFKYQTDQFTGEKGAKD"
BASE COUNT          516 a          691 c          598 g          582 t
ORIGIN      
        1 ctcctcgagc gtggccaggg tcaggagatc aaggtcgttc gtaggttcgt cgaggatcag
       61 gaaattcgga ttgcggatca gcaccgtgag caaatgcaga cgtcgccgct cgcccccgct
      121 cagcttcgag acgtaggtct gctgcaacga aggctgaaac tgaaacaact gcagaaactg
      181 tccggccgac accttgctgc catcggccag ttgaatcacg tcggcatgtt cccgaacgac
      241 ttctatgacg cgtttatcct ccttcacctg caatcccgcc tgtgaataga acccgaatac
      301 gacggtctcg ccaatattca ctttcccgga gtccggcggc tcctttccgg agatgatgtt
      361 caggaaggtg gtcttgccac agccgttctt tccgacgatg ccgacccgtt caccggtccg
      421 gaacgtatag tcgaagcctt tcaggatggt acgatcgcca aacgccttgt agactttttt
      481 caactcgatg atcttcccgc cgatcctgtt catcttcaca tcaagctcaa gaccgtcttc
      541 ctttcgcttg ccacccatac ggtctttcag atcatgaaag gcatcgatcc gcgctttcga
      601 cttggtgcca cgcgccttcg gcatccgacg cacccattcc agttcggtcc tgaagaggtt
      661 acgcaatttg tcctgggtcc gggattccgc ttcctcccgt tcggccttct tctccaggaa
      721 gaacgcgtag ttaccatcat aagcgaagat cttccccaga tcaagttcga ggatgcggtc
      781 ggtgacatta tccaggaagt aacggtcgtg ggtcacgagt aacaggctga ggttggcccg
      841 gccgagatag ccctccagcc attccaccat ttcaacatcc aggtggttcg tgggctcgtc
      901 gaggatgagc aaatcggagg gttctaccag caccttggcc agtgcgattc gtttttgctg
      961 accgccggag aaacccagga tctttcggtc gaggtcgtga atgtcgagtt gtccaaggac
     1021 ctgacggatg cgggcttcat agtcccaggc attggccgca tccatgcggt gtgtcatctc
     1081 ttccagattc ctcgcgcttg ccggcgtgtt ctcccgggag tgttgttcca gcgccagttc
     1141 gtattgccgg gtgagttgta ctaccggatt ctcgtcaccg aagatcagct ccgcgacggt
     1201 tcgatgtccg tcgaaactgg gctcctgttc gaggtaagca acgcgaacat ccttccgata
     1261 cgtgacctca ccggaatccg gcacgtcttt accggccaga atgcgcaaca gggtggtctt
     1321 gcctgtaccg ttccgcgcga tcaaagctac cttctcgcca cgcgcgatcc cgaaactcaa
     1381 ctcctggaag aggagtttgt caccataagc cttgctgaca cgatcaaccg ataaaagatt
     1441 cattgctcaa atttccttcc gcaaggcact actgattagt cgattaatac tctttcgaat
     1501 tcacatccag ctaccgcaaa ctgctcaccg cataccgccg aatgaaatcc gccaaccgaa
     1561 ttcaaattcc ttaatccttc gcgcctttct caccggtgaa ctgatcggtc tgatacttga
     1621 atagcacatt gaacgtggtc aggctgccat aacaacgggt gatgtactgc tggatgttga
     1681 ccttgtcctc atccgacaac ttttcatggg cattgatgcg ttgctccagc acccgcaggc
     1741 gatcccgcag catcacaatc ttgttgaaga acgtttcaat cgggacctcc ttgggtttca
     1801 acgcggcatc ggccggttgt agcaccatct tgccccctac ccaacgacct cccaactcga
     1861 cacgctcctg gatgtcggag tacttccgga tcacattaac caacaacacc tcgatatcgg
     1921 ccagtgacac caggtcctgg tcctgatcgt tggcgtcgat gatctcaagg ccctgttcgt
     1981 cacggtccag atcgcgcatc ccgtgatcca tgaaagttac gacatactct tccggtttga
     2041 cctgtacgat gacgcctgca ccaaactggg gatgccggat ccgggaacct attccgaggg
     2101 agttggtttt catgggagcg atttttcaca aaaatactcc tttccccgca gcccactgac
     2161 aaagcccgaa tcatcggtat taacagtccg gaggttaagt tgtgttaatg tctgtcacga
     2221 acaggtcatc tcgaacgtct catacaatac aagcatgcac aggacgttgt tctcattacg
     2281 tttttgtgtt catgtttttc gtgtgggggg ccgtcggttc gaaaggatcg gcggctcttt
     2341 gtttgaagga gggacaagaa ccaagggacg agggacgagg aatgagg
//
LOCUS       DAOTON010000437         2384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_34760_length_2384_cov_2.590812, whole genome shotgun sequence.
ACCESSION   DAOTON010000437 DAOTON010000000
VERSION     DAOTON010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34760_length_2384_cov_2.590812"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1555
                     /locus_tag="P5292_08785"
     CDS             <1..1555
                     /locus_tag="P5292_08785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HRS39260.1"
                     /translation="GHGCYVAGIGSGDGSSVGHFQGVAPKSDIIVVNYDFTAQDTLPR
                     VAHAVEYIFNKASLLGKPCVINASLGDYYGSHDGRDLQSQYIRNLIIAQPGRALVAAA
                     GNVGIVYPFHVGTTTTAGDTSFSWFKYNSGIPGAYCQIFADTADFNNISFAIGADKVT
                     PDFSFRGRTAFRNIQNTLGLVNLNLVNGGNRIGRIQLYGSESDGVYSLEVYVIPDSTT
                     YHWRFETTGNGHFDSWTFDWVFQNLPAPGVFPDIVNYNPPDTNQSIVSGFACLDEVLT
                     VGNYYNTDRHIDYNGTLQVSPGDRPRQLAENSSRGPTRDGRIKPDICAPGHHILSTGV
                     TTLIPGMIAAQPWKVAPGGYHVTGGGTSASAPVVAGVAALYLQKNPGATYQDIKDAIL
                     GCAMTDSLTWGPYPNAAWGFGKVDAFGVMTACGAVGLSDEPTPDNALLVYPNPADGFV
                     TVQIADPSYAGKGRIRIFDISGRLVLEPAMVQGVFKFSTENFPSGSYLIQFTDDAGNT
                     SRQPLIIRH"
     gene            1657..>2384
                     /locus_tag="P5292_08790"
     CDS             1657..>2384
                     /locus_tag="P5292_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRS39261.1"
                     /translation="MSNPRFPARLLDPVRSLLKVPSKIVITTHHRPDGDAMGSSLGLY
                     HWLLSQGHTVRVVAPSDYPDFLQWLPGNDQVLQFTREPEQCIEHFNAAAIIFCLDFNW
                     MSRVEQMEPVLRGAAAVKVLIDHHLDPEPAFDHIFSYPEACSTSELIYQFIVSLGGRD
                     QVSKASAECLYCGIMTDTNSFRYESMRSDTHRIIADLMDAGAVNYRIHELVYDNFTEN
                     RLRLMGYVLKDKLTVLHEYRTAYIA"
BASE COUNT          501 a          685 c          617 g          581 t
ORIGIN      
        1 tgggcacggt tgttacgttg caggcatcgg ctccggtgac ggttcgtcgg tcgggcattt
       61 ccagggcgtt gcgcccaaga gcgatatcat tgttgtcaac tatgacttca ctgcccagga
      121 caccttgccg cgcgttgcgc atgctgtcga gtatatcttc aataaggcct cgctgttggg
      181 aaaaccctgt gtgatcaatg cctccctggg cgattactac ggttcacacg acggccgtga
      241 tctccagtcg caatacatcc gcaacctcat catcgcgcaa cctggacgag cgctggttgc
      301 cgccgccggg aatgtgggta ttgtttatcc gtttcatgtg ggtacaacaa caacggcagg
      361 cgacacgtcc ttttcctggt tcaaatacaa cagcgggatc ccgggtgctt attgccagat
      421 cttcgcggat acggccgatt tcaacaacat atctttcgcg atcggagccg ataaggtcac
      481 gcctgatttt tccttccgtg gacgtaccgc tttccggaac atccagaata cacttggact
      541 ggtcaacctg aatctggtca atggcggtaa tcgaattggc cggatccagc tttatggttc
      601 cgagagcgat ggagtctatt ccctcgaagt ctatgtgatc ccggattcaa cgacatacca
      661 ttggcgtttt gaaacaaccg gtaacggaca ttttgattcc tggaccttcg attgggtgtt
      721 ccagaatctt ccggctcccg gagtatttcc ggatatcgtc aattacaatc cgcccgatac
      781 taaccagtcg atcgtgagtg gctttgcctg cctggatgag gtactgaccg tcggcaacta
      841 ctacaatacc gatcgccaca tcgactacaa cggtaccttg caggtttctc ccggtgaccg
      901 cccgcggcaa ttagccgaga atagcagccg cggaccaacg cgcgacggca ggatcaaacc
      961 ggatatctgt gctcccggtc atcatatcct ttcaaccgga gtgactaccc tgattcccgg
     1021 gatgatcgcg gctcagccct ggaaggtcgc tccaggcggc tatcatgtga cgggaggcgg
     1081 tacctctgct tcagctcccg tggttgccgg tgttgcagcg ctttacctcc aaaaaaatcc
     1141 gggagctact tatcaggata tcaaagacgc gatcctgggt tgtgccatga ccgactcact
     1201 gacctggggg ccctatccga atgcggcctg gggctttgga aaggtggatg ccttcggcgt
     1261 aatgaccgct tgtggcgcag tgggattgtc ggatgaaccg acacctgaca atgcattgct
     1321 ggtttatcct aatcctgccg atggatttgt gaccgttcaa atcgccgatc cttcctacgc
     1381 cggcaaaggg cgcattcgga tcttcgacat ttccggaagg ctcgtcctgg agcccgcaat
     1441 ggtgcagggt gttttcaaat tttcgaccga aaacttcccg tcaggttcct acctgatcca
     1501 attcaccgat gatgcaggta acaccagccg ccagccactg atcatccggc actgatcatt
     1561 ccgggaacac caattcgaat acaccgggcc cgcctgcgtt cgtaagaacc ggcgggcccg
     1621 atcgttctat acaaccggaa ctcgtatttt tggcacgtga gtaatcctcg ttttcctgcc
     1681 cggcttctgg atccggttcg ttccctgttg aaggtgccgt caaaaattgt cattaccacc
     1741 caccaccgtc cggatggtga tgccatggga tcctccttag gattgtatca ttggttgtta
     1801 tcccaggggc acaccgttcg ggtcgtcgcc cccagcgatt atccggattt cctgcaatgg
     1861 ttgcccggta atgatcaggt cctgcaattc acccgtgagc cggaacagtg catcgaacat
     1921 ttcaatgctg cagcgatcat cttttgcctc gacttcaact ggatgagtcg ggtggagcaa
     1981 atggaacccg ttctccgcgg agccgctgcg gtgaaagtac tgatcgatca tcacctggat
     2041 ccggagcccg ccttcgatca cattttctcc tatccggagg cttgcagcac gtcggaactc
     2101 atctatcagt tcatcgtcag tttgggcgga cgtgatcagg tttccaaagc atccgccgaa
     2161 tgcctctatt gcggaatcat gaccgatacc aattcgttcc gctacgagtc gatgcgctcc
     2221 gatacccacc ggatcatcgc cgacctgatg gatgccggag cggtcaacta ccggatccac
     2281 gagctggtct atgataattt caccgagaac cgcctgcgcc tgatggggta cgttctcaag
     2341 gataaattga ccgtcctcca tgaataccgc acggcctaca tagc
//
LOCUS       DAOTON010000438         2381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_34806_length_2381_cov_2.918745, whole genome shotgun sequence.
ACCESSION   DAOTON010000438 DAOTON010000000
VERSION     DAOTON010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34806_length_2381_cov_2.918745"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="P5292_08795"
     CDS             complement(<1..469)
                     /locus_tag="P5292_08795"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRS39262.1"
                     /translation="MVFLTLLAGSGPLYGQHLTRFYMKAKVGYRDDKGAIVVPATYDA
                     GSEFIEGRALVVRDNLRGFLDVFGTEVIPLQFTDAGVFNKGIARVARDGKYGWIRPDG
                     SYSIPAQFDLADEFCDGLARVMIGGKFGYINRDGQTIIALQYQNARNFSSGLAP"
     gene            complement(604..>2381)
                     /locus_tag="P5292_08800"
     CDS             complement(604..>2381)
                     /locus_tag="P5292_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS39263.1"
                     /translation="VGMINVDFIREVDFYSSAFPAARGNTLSSVMDFKLKEGRAERMG
                     YTLTLGASDLAATVDGPLSEKVNFIASWRRSYLQFLFKAIGLPFLPTYDDFLVKTKWK
                     IDDRNEITFLGLGADDRVRLNLEQDETDLQQYLLNNLPVNTQWNYTVGAVYRRYRTNG
                     YSLFALSRNQLQNDAEKFQDNDASSENNRLLDYSSRETENKFRYEQVERIGNQKLTFG
                     MNLESAEYTNRTFNRVPYVGEVRYQSDISFYKAALFAQWSISALREDLTLSAGFRLDA
                     NTFNDRMQRPQDQFSPRLSLSYRLSDRFRFNANAGRYFQLPAYTILGFRDNAGTLVNK
                     VDARYMQCDHLVAGFEYTAPSFVRITLEGFYKNYSQYPFNLRDSISIANQGSDFGTVG
                     TVPVRFDNRGRSYGVEFMVQQKLFKKVFGILAYTLLRSEFEGLDGRYLPSSWDFRHVV
                     SLTGGKYFPKNWQVGFRFRFNSGTPYTPFDVAASVEKSNFDITGQGIQDRSAINSRRT
                     DAFHQLDLRIDKKYYYKKWSLNFFLDVQNVYNQVTEFAPYVSVQRNANGQPIVDPNNT
                     DAYLPLLIPNEAGSLIPSIGIVAEF"
BASE COUNT          586 a          607 c          628 g          560 t
ORIGIN      
        1 ccggtgcaag tccggaagaa aaattccggg cattctgata ttgcaacgcg atgatcgtct
       61 gtccatcccg attgatgtaa ccgaatttcc caccgatcat cacccgtgcg agtccgtcgc
      121 aaaactcatc cgccaggtcg aactgcgcgg ggatcgaata cgaaccatcc ggtctgatcc
      181 aaccgtactt accatccctg gctacgcgtg caatcccttt gttgaacaca ccggcatccg
      241 tgaactgcag cggaataacc tccgtgccga agacatcgag aaagccacga agattatcgc
      301 ggaccaccag ggcccttcct tcgatgaatt cggaaccggc atcgtaggtg gccggcacaa
      361 cgatagcacc cttgtcatcc cggtagccga ctttggcttt catgtaaaac cgggtgaggt
      421 gttgtccata caacggcccg gaacctgcca gcagggtcag gaaaaccatt aaatgaagga
      481 atcggaatct tcccatcgca tttgaattta gttaccaaat gtaaccgaat gcggtaatcc
      541 ggcaaggaaa ggacggacct tattttgcca acgcgggaat atggagggcg aagggcagaa
      601 agatcagaat tcagccacga ttccgatact tggaataagg ctccccgctt cattggggat
      661 cagcaagggc aaataggcgt cggtattatt gggatctacg atcggctgcc cgttggcatt
      721 ccgttgtaca ctgacgtaag gtgcaaattc cgttacctga ttatagacat tctgcacatc
      781 caggaaaaag ttcaagctcc attttttata gtaatacttc ttatcgatcc gcaggtcgag
      841 ctgatgaaac gcatcggtgc gccgggaatt gatggccgaa cggtcctgta tcccttgtcc
      901 ggtgatgtcg aagttggatt tctcgacaga cgctgcaacg tcaaaaggcg tataaggtgt
      961 accactgttg aatcggaagc gaaaacccac ctgccaattc ttgggaaagt acttgccgcc
     1021 ggtcaggctg acgacatgcc ggaaatccca ggaagacggc agataccttc catccaagcc
     1081 ttcaaactcg ctgcgcaaga gcgtatacgc caggatgccg aacactttct tgaacagttt
     1141 ctgttgtacc atgaattcga ccccatagga acgcccccta ttgtcaaaac gaacaggtac
     1201 agttccaacg gtaccgaaat cgctgccctg gttggcgatg gaaatggaat cgcgtaggtt
     1261 gaagggatac tgcgaatagt tcttgtagaa tccttcgagg gtaattcgga caaaggaggg
     1321 tgccgtgtat tcgaaaccgg cgaccaggtg atcgcactgc atgtagcggg catccacctt
     1381 attgaccaag gttcctgcat tatcgcggaa gccaagaatg gtataggccg gaagttggaa
     1441 gtaccgtccg gcgttggcat tgaaacggaa gcggtcggaa agccgatagc tgagcgacag
     1501 tcggggagaa aactgatcct ggggacgttg catccggtcg ttgaacgtat tggcatccag
     1561 acgaaatccg gctgatagtg taagatcttc ccggagagcg gagatactcc actgcgcgaa
     1621 gagggcagct ttgtaaaagc tgatgtcgga ttgataccgg acttctccca catacggcac
     1681 cctgttgaag gtccgattcg tatattcggc cgattccagg ttcatgccga aggttagttt
     1741 ctggtttccg atcctttcca cttgttcgta cctgaacttg ttctccgttt cccggctgga
     1801 ataatcgagc aagcggttgt tctcagaaga cgcatcgttg tcctggaatt tctccgcgtc
     1861 attctgcaac tggtttcttg aaagtgcaaa cagggaatat ccgttcgttc ggtaccggcg
     1921 gtaaacggct ccgacggtgt aattccactg cgtgttgacc ggaaggttat tgaggaggta
     1981 ttgctgcaaa tcggtttcgt cctgttctag gttgagtcgt acccggtcat cggcaccaag
     2041 acccaggaag gtgatctcgt tacgatcatc gatcttccat tttgtcttga cgaggaaatc
     2101 atcgtaggtc ggcaggaatg gcaaaccgat cgctttgaag aggaattgca ggtagcttct
     2161 tctccaggat gcgatgaaat tcaccttctc ggacaagggc ccatccacgg tcgctgccag
     2221 gtcggatgct cccaacgtca gggtgtaacc cattcgctct gccctgccct ctttcaactt
     2281 gaaatccatg accgagctga gtgtgttacc gcgagctgcc gggaaagcac tgctgtaaaa
     2341 gtcaacttcc cggatgaagt ctacgttgat catgcccacg g
//
LOCUS       DAOTON010000439         2376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_34903_length_2376_cov_3.812150, whole genome shotgun sequence.
ACCESSION   DAOTON010000439 DAOTON010000000
VERSION     DAOTON010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34903_length_2376_cov_3.812150"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="P5292_08805"
     CDS             <1..270
                     /locus_tag="P5292_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HRS39264.1"
                     /translation="KDGRWEIRNPLDNRCWKLWHSAVVTWDGRVVPCCFDKDAQHVMG
                     HLEKRSFREIWNGPEYAVFRNQLIKGRSEIDICRNCSEGLSVFTE"
     gene            338..1243
                     /locus_tag="P5292_08810"
     CDS             338..1243
                     /locus_tag="P5292_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRS39265.1"
                     /translation="MIAKLIKTFTGHDGSIYALEGSGREDRVFTAGGDRIVSEWDLSG
                     NEPARGVVKVEAVVYALCHIPERNWLLVGTSEGRMHVIDLEGRKELHNLELHKAGIFD
                     LRFHPTTGNIFSASGDGSIAVLQLDRLDRTERRSLAPGHKIRAIDFHPLHPEVAFACG
                     DGNIRIYSLERFEERLVFPAHALSANCVRYHPAGNYLLSGGRDAHLCAWDPATGASLA
                     RIPAHNYAIYSIAFSPDQHNFATASRDKTIKIWDAETIGFRLRIDRDTYNGHRNSVNK
                     VLWSRYDNYLISAGDDKAVLVWDVV"
     gene            1463..1858
                     /locus_tag="P5292_08815"
     CDS             1463..1858
                     /locus_tag="P5292_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39266.1"
                     /translation="MRVVAVQRGKRELALVVVLFIIMTAVLPQFQDVILPPETRFRLF
                     GIAGGNKRLVILGPALGLLFAIGLFRRKFWARQLTLLVCIIVAVTAIAFIIASDAYYY
                     PGQIGLLVVAATCSALLRFNKNIRFYFEA"
     gene            complement(1895..1970)
                     /locus_tag="P5292_08820"
     tRNA            complement(1895..1970)
                     /locus_tag="P5292_08820"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1935..1937),aa:Thr,seq:cgt)
BASE COUNT          569 a          566 c          643 g          598 t
ORIGIN      
        1 aaagacggac gctgggagat ccgaaatccc ctggacaatc gttgctggaa actttggcat
       61 tccgcagtgg ttacctggga cggacgagta gtcccctgct gctttgacaa ggatgcccaa
      121 cacgtcatgg gccatttgga gaagcgttcc ttccgcgaga tctggaacgg ccccgagtac
      181 gcggtattcc ggaatcagtt gatcaaaggt cggagcgaga tcgatatttg ccggaactgc
      241 tcggagggcc tatcggtctt tacagaataa gaaaggttgt agaaggcggg aggtattgtt
      301 aatcaatcat cgaatttgaa taattgatac tgtaaaagtg attgcgaagc tgatcaagac
      361 atttaccggg cacgacggtt ccatctatgc actcgaaggt tctggtagag aagaccgggt
      421 ctttaccgct ggtggcgacc ggatcgtatc cgaatgggac ctcagcggga acgaacccgc
      481 aaggggagtg gtcaaggttg aagcggtggt gtatgcgctt tgtcacatcc cggaacgcaa
      541 ctggttgttg gtgggaactt ccgaaggtcg catgcatgtg atcgacctgg aaggacgaaa
      601 ggaactacac aacctggagt tgcacaaagc cggtatattc gacttgcgtt ttcacccgac
      661 aaccggaaac atcttctccg cttcgggaga cggcagtatc gccgtcctgc agcttgaccg
      721 attggacagg actgaacgca ggtcgcttgc tccagggcat aagattcggg ctatcgactt
      781 tcaccccctt catccggaag tcgcctttgc ctgcggggat ggaaacatcc ggatctattc
      841 gctggagcgg tttgaagagc gtctggtctt tccggcgcac gcgctttccg ctaattgcgt
      901 acgctatcat cccgcgggga attatctgct cagcggagga cgggacgccc atttgtgtgc
      961 ctgggatcct gcaaccgggg cctcgttggc aaggattccg gcgcataact acgcgatcta
     1021 ttccatcgca ttttcgccgg accaacacaa ctttgcaacc gccagccgcg acaagaccat
     1081 caagatatgg gatgctgaaa cgatcggttt tcgactgagg attgatcgtg atacgtacaa
     1141 cgggcaccgc aattccgtca acaaagtgct ctggagccga tacgacaact acctgatctc
     1201 cgcgggcgat gataaggcgg tgttggtgtg ggatgtggtg tgagcggttg ttagtgaata
     1261 gtgaatagta tggagcgaat agccaatagc gaacagcgaa tagttttaat gaatagtgaa
     1321 ccctgcctgc cgtagcttta gcgtaggcag gagtgaatag taaatagcga atagccaata
     1381 gcgaacagcg aatagtttta atgaatagtg aaccctgcct gccgtagctt tagcgtaggc
     1441 aggaaacccg gaaccccttc ccatgcgtgt agtagctgtc caacgtggta aacgagaact
     1501 tgctctggtt gtcgtgttat tcatcatcat gaccgcggtg ttaccgcaat tccaggatgt
     1561 gatactaccg ccggagacgc gtttccgttt gtttggtatt gccggtggaa acaaacgatt
     1621 ggttattctg ggaccagctc tggggctgtt gtttgcaatc ggactcttcc ggagaaaatt
     1681 ctgggcacgt caactgactt tgctggtctg catcatcgtc gcggtgacag ccattgcttt
     1741 catcattgct tccgacgcgt actattatcc cggccagatc gggctcctgg tggttgccgc
     1801 tacctgctct gcgctgttgc gcttcaacaa gaatatccgc ttctacttcg aagcataaaa
     1861 aagaaaaacc gttcacggta ggtgaacggt tttgtggagc caattgtcgg actcgaaccg
     1921 acgacctgct cattacgaat gagctgctct accagctgag ctaaattggc tggttaaaag
     1981 acgctttatt agcgtttttg gaggcgcaaa gttccgaaaa aaaccctttg aatgtctcat
     2041 tttgacggta ttttttgcga attccgttca aaatgcgggt ttttaccaaa aaatcaagaa
     2101 aactgaaaca aacagccggt cagcccgttc aaattgctgt gtgtttcatg ttaagtttta
     2161 agtaagtact tgagttgttg atcaggaaac cccggacgga agtcatgggg tttcttgttt
     2221 ttcataggta ccgtcggtgg ttcattaacc gctctgcccc ggattctcgt cgaaggttag
     2281 ttgttccgag ttccgtgttt cgcgtttcga gttaatgaaa atcctcgcac ctcgctccac
     2341 gcatctcgtc cctcataaaa gacccgaaac tcgaaa
//
LOCUS       DAOTON010000440         2376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_34908_length_2376_cov_3.285222, whole genome shotgun sequence.
ACCESSION   DAOTON010000440 DAOTON010000000
VERSION     DAOTON010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34908_length_2376_cov_3.285222"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="P5292_08825"
     CDS             <1..634
                     /locus_tag="P5292_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HRS39267.1"
                     /translation="VTTGAPGLFVPSTQTADNITFFTDDGSSLYYNILLNLSSFDTWW
                     DNQAQVPYLTGRNGLNTFVSYDDEQSIARKAQYINDHNLRGAIIWEITGDYLETSPGS
                     GIIAATPLADTLNAVLCSSTGLSGVSGVDEWQLYPSPAIDRVHVRGAAQDQIISASML
                     TAYGQFVDVMVRERKEEIVFDVRSLPAGLYLVELRCKDGARRVLRFPVAR"
     gene            complement(643..1218)
                     /locus_tag="P5292_08830"
     CDS             complement(643..1218)
                     /locus_tag="P5292_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HRS39268.1"
                     /translation="MEKNRLEAFSDGVLAIIITIMVLELKVPHGTNWTDLLALWPVFI
                     SYVLSFLYIGIYWGNHHHLMHTARRVNSGILLTNLHLLFWLSLIPFTTAWMGENHFES
                     NPVALYAVNLTAAGIAYFILQKSIERHHAKDDQLKSAFQRHAKKGIVSQIAYAAAIPL
                     AYVHPMISGIIFLLIAILWIVPAKEIEEAIK"
     gene            complement(1225..1716)
                     /locus_tag="P5292_08835"
     CDS             complement(1225..1716)
                     /locus_tag="P5292_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRS39269.1"
                     /translation="MLPEYRTLLEQAVNRKKENKAFLERLKKSRPADLDQTTNRLHDE
                     AFEHIDCLQCGNCCRTTGPLLLSKDVDRLAGHFRIRPAEFTERHLRIDEDGDYVFRSM
                     PCPFLGGDNYCSVYENRPNACREFPHTQQRDIREKLGITYYNTMVCPAVAEVVERLKA
                     HYR"
     gene            complement(1809..>2376)
                     /locus_tag="P5292_08840"
     CDS             complement(1809..>2376)
                     /locus_tag="P5292_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRS39270.1"
                     /translation="TSDYDREKLQERLAKLAGGVAVLYVGAATEVEMKEKKDRVDDAL
                     HATRAAVEEGIVPGGGVAYIRAINGLDKLAGANEDETTGVAIVKRALEEPLRQIVANA
                     GMEGSIIVQKVREGKDDFGYNARSDKYENLYSAGVIDPTKVTRVALENAASIAGMLLT
                     TECVLADIKEEKAAPAMPHGGMGGGMDY"
BASE COUNT          491 a          627 c          687 g          571 t
ORIGIN      
        1 cgtcaccacg ggtgcgccgg gattgttcgt tccttccacg caaaccgctg ataacatcac
       61 gttctttacc gatgacgggt cgtcgctgta ttacaacatt ctcctgaacc tttcttcctt
      121 cgatacctgg tgggacaatc aggcccaggt gccctatctg accggccgga acggattgaa
      181 caccttcgtc tcctacgacg acgaacaatc catcgcgcgg aaggcgcagt acatcaacga
      241 ccataacctt cggggcgcca tcatctggga gatcaccggc gactatttgg aaacaagtcc
      301 gggttccgga atcatcgcag cgactcccct ggccgataca ctcaatgccg tgctctgcag
      361 tagtaccggc ttatcaggcg tttctggtgt ggatgaatgg caactctatc cttcaccggc
      421 gattgatcgg gtgcatgtcc ggggagcagc acaggatcag attatcagcg caagcatgct
      481 gacggcttac ggacagttcg ttgatgttat ggtgcgtgaa cgcaaagaag aaatagtatt
      541 cgatgttcgt tccttacctg ccggcctgta tctcgttgag cttaggtgta aggatggagc
      601 aaggcgcgta cttcgatttc cggtcgcgcg ttaacgacag cattatttga ttgcttcttc
      661 gatctctttg gctggaacga tccagagtat ggcgatgagt agaaagatga tgccggagat
      721 catgggatgt acatacgcca gcggaatggc tgccgcgtaa gcgatctgcg atacgattcc
      781 tttttttgcg tgcctttgaa aggccgattt caactgatcg tccttcgcgt gatgacgttc
      841 gatggatttc tgaagtatga aatacgcgat acccgcggct gtcaggttga ccgcatacaa
      901 ggccacggga ttcgactcga aatgattctc ccccatccag gccgtggtga agggaatgag
      961 cgatagccaa aacaacagat gcaggttcgt gagcaggatg ccggagttca cgcggcgtgc
     1021 ggtatgcatc aggtgatggt ggttacccca gtaaattccg atgtacagga aactcaggac
     1081 atagctgatg aataccggcc aaagcgccag caggtctgtc cagttggtac catggggtac
     1141 cttcaattca aggaccatga tggtgataat gatggccagc acgccatcac taaaggcttc
     1201 cagtcggttc ttttccattt tgaattatcg ataatgtgct ttcaggcgtt ccaccacttc
     1261 ggcgactgcc gggcagacca tggtattgta gtaggtgatc ccgagctttt cacggatgtc
     1321 gcgttgttgg gtgtgtggaa attcccggca ggcgttcggg cggttttcat agacactgca
     1381 atagttgtcg ccgccaagaa acgggcaggg catcgaccgg aacacgtaat cgccgtcctc
     1441 gtcgatccga agatgacgct cggtgaactc ggcgggacgt attcggaaat gtccggccaa
     1501 tcgatccaca tccttgctca ggagcagggg gccggtggtc cggcaacagt tgccgcattg
     1561 caggcaatcg atgtgttcga atgcctcatc gtgtaaccga ttggtcgtct ggtccaggtc
     1621 ggccggtcgg ctcttcttca accgttcgag aaaggctttg ttttccttct tccggttaac
     1681 ggcttgttcc agtaacgtgc ggtattcggg cagcattcag tgggagatcg cttaaagata
     1741 atgggcgggg aaataaaaaa gaaaaccccc gccggatggc aggggtttct tttctgaagc
     1801 agtggatctt agtagtccat accgccgccc ataccgccgt gcggcatggc aggagcagct
     1861 ttctcctcct tgatgtcggc gagtacgcac tcggtcgtca gcaacatgcc ggcgatcgaa
     1921 gcggcgttct cgagcgcgac gcgggtcacc ttggtcgggt cgatgacacc ggccgagtag
     1981 aggttctcgt acttgtcgga gcgggcgttg taaccgaagt cgtctttgcc ttcacgaact
     2041 ttctgtacga tgatcgaacc ttccatgccg gcgttggcta cgatctgacg cagcggctct
     2101 tcgagcgcgc gtttcacgat ggcgacaccg gtggtttcgt cttcattggc gcccgccagt
     2161 ttgtcgaggc cgttgatggc gcggatgtaa gcgacaccgc cgccggggac gataccttct
     2221 tcaacagcgg cacgggtggc gtgcagcgcg tcgtcgacac ggtctttctt ctctttcatt
     2281 tcaacttcgg tcgcggcgcc tacgtagagg acggccacac cgccggcgag cttggccagg
     2341 cgctcctgga gtttctcgcg gtcgtaatcg gaggtc
//
LOCUS       DAOTON010000441         2375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_34932_length_2375_cov_3.016379, whole genome shotgun sequence.
ACCESSION   DAOTON010000441 DAOTON010000000
VERSION     DAOTON010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34932_length_2375_cov_3.016379"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="P5292_08845"
     CDS             complement(<1..1022)
                     /locus_tag="P5292_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRS39271.1"
                     /translation="MIGQLIFLLLLLATVAVFTRNIRKVIRNIRLGRPLDRSDRSADR
                     WKVMTMVALGQSKMVTRPVAGILHIFVYAGFVIINIEVLEIVIDGLFGTHRVFAFLGP
                     LYDLLIGSFEILALLVLVACVIFLVRRYVIRLKRFWMREMTSWPRTDATLILVTEILL
                     MSAFLKMNAVDSVLQAQHYGHYVQAGWFPVSSMLTPFFEGWNPENLVIVERLLWWFHI
                     IGILVFLNYLPYSKHFHILLAFPNTYYSNLDYKGRFTNMESVTREVKLMLGPSAVPPG
                     DAAAPPSRFGAKDVFDLSWKQLMDAYSCTECGRCSSNCPANQTGKLLSPRKIMMDTRD
                     RLEEVG"
     gene            complement(1032..1991)
                     /locus_tag="P5292_08850"
     CDS             complement(1032..1991)
                     /locus_tag="P5292_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRS39272.1"
                     /translation="MKISKEARIGLIVTVGIAALFWGINYLKGKDFFTSQKLVYAVYD
                     HVDGLAPSNTVQVNGMKVGMVKSLSLFPDRSGRILVSMHLKNEVVVPRNSTAEIFGTD
                     LLGTKGIRLVLGDATEELQDGDTLVSAIQQSLSESVSAQVAPIKQKAENLLSSMDSVL
                     IIVRTVFNEKTKDNLKRSFESISNSLLSIEHVTGSLDTGLTANGKLASMLASLESILA
                     NFRKNNDAITNAINNFSSISDTLARANLAMTIENTRKTLEETSSLLRKVNTGEGSLGQ
                     LATNDSLYHNLNSTSRSLDMLLTDFRENPKRYFNVSLISFGKK"
     gene            complement(2142..>2375)
                     /locus_tag="P5292_08855"
     CDS             complement(2142..>2375)
                     /locus_tag="P5292_08855"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRS39273.1"
                     /translation="VSPTRLTSSSSKLKKAGDWREEKDGKLYKYVSGKFRDPEDALDL
                     QTALRAKGYKDAFVVAYEGVKRIPYKEAKARLK"
BASE COUNT          572 a          655 c          617 g          531 t
ORIGIN      
        1 ttacccactt cctccaagcg gtcgcgcgta tccatcatga tcttacgggg cgaaagcaac
       61 ttgccggtct ggttcgcggg acaattcgag gaacaacgcc cacactccgt acaactgtaa
      121 gcgtccatca attgcttcca gctcaggtca aacacatcct tggctccgaa ccgcgatggc
      181 ggagctgccg cgtctcccgg tggcacggcg gacggaccca gcatcaactt cacctctcgc
      241 gtaacacttt ccatgttggt gaaacgccct ttgtagtcga ggttggaata ataggtattc
      301 gggaaagcca gcagaatatg aaagtgtttg gaatacggca ggtagttgag aaaaacgaga
      361 attccgatga tgtggaacca ccaaagcagg cgctccacga tgaccagatt ttcagggttc
      421 caaccttcga agaacggcgt caacatactg ctgacgggga accaaccggc ctgaacatag
      481 tgaccgtaat gttgggcctg taacacggaa tcgaccgcgt tcatcttcag gaaggccgac
      541 atcaacagga tctcggtcac caggatcagg gttgcatcgg tcctgggcca gctggtcatt
      601 tccctcatcc agaaacgctt caggcgaata acataccggc ggaccaggaa aatcacgcaa
      661 gctaccagta ccagcagcgc gaggatctcg aaactcccga tcaggagatc atacaagggt
      721 ccgagaaatg caaacacccg atgcgtacca aacagtccgt cgatgacgat ctccagcact
      781 tcaatgttga tgatcacgaa tcccgcgtaa acgaatatgt gcaggatccc ggccaccgga
      841 cgggtcacca tcttcgactg acccagagcg accatggtca tcaccttcca gcggtcagcg
      901 ctgcggtcag aacggtcgag cggtcgcccg agccgaatgt tgcggatgac tttccggata
      961 ttgcgcgtaa agaccgcaac cgtggccaga agcaagagca ggaatatcaa ttgtccgatc
     1021 atacgtccgg attatttctt gccgaatgag atcaatgaaa cattgaagta ccgcttgggg
     1081 ttttcccgga agtcggtcaa gagcatatcc aggctgcggg acgtggaatt caggttgtgg
     1141 tagaggctgt cgttcgtagc cagttgcccg agcgaacctt ccccggtgtt gacctttcgc
     1201 aaaagcgagg aggtctcttc cagtgtcttc cgggtatttt caatggtcat ggcgagattg
     1261 gcgcgggcca gggtatcgct gatcgacgag aaattattga tcgcgttggt gatcgcgtca
     1321 ttgttcttcc ggaaattggc caaaatggat tccagggaag caagcatgct tgccagtttc
     1381 ccgttggcgg taaggccggt atccaggctg ccggtcacgt gctcgatcga gagcagcgaa
     1441 ttgctgatgc tttcaaatga acgcttgaga ttgtctttgg ttttttcgtt gaagaccgtc
     1501 cggacgatga tcaggaccga atccatggag gacaagaggt tctccgcttt ctgcttgatc
     1561 ggtgcgacct gggcactgac cgattcgctg agactttgct gaatagccga cacgagggta
     1621 tcgccgtctt gcaactcttc ggtcgcgtcg cccagtacca gccggatacc tttcgttccc
     1681 aacaggtcgg ttccgaagat ctcggcggtg gaattacgcg ggaccactac ttcgttcttc
     1741 aaatgcatac tgaccaggat acggccggag cggtcaggga agagcgaaag gcttttcacc
     1801 atgccaacct tcatcccatt cacctgaacg gtgttgctgg gcgccaggcc atcgacgtga
     1861 tcataaaccg cgtacaccag tttctgactg gtaaagaaat ctttgccttt caaataattg
     1921 ataccccaga aaagggcggc gatccctacc gtgacaatca gtccgatgcg tgcttccttc
     1981 gaaattttca tacccaatag accggaagta cgatcctgcc ggtcaaaacc gtgattgatt
     2041 tagcgccgaa aggtaggcat tttagcccgg cctgctttcc gcgacgctca tccgaaagtc
     2101 cgaaaattca acaggatatg aacaaaagcc ggcacacctc cctacttcaa ccgggctttg
     2161 gcttccttgt acggaatacg ttttacgccc tcataagcga ccacaaaagc gtccttataa
     2221 cccttcgccc tcaaagccgt ttgcaggtca agcgcgtctt ccggatcacg gaatttcccg
     2281 ctgacatact tatagagctt cccatccttt tcctcccgcc agtcccccgc ctttttcaac
     2341 ttggaactcg aggacgtcag gcgggtgggc gaaac
//
LOCUS       DAOTON010000442         2373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_34979_length_2373_cov_2.618205, whole genome shotgun sequence.
ACCESSION   DAOTON010000442 DAOTON010000000
VERSION     DAOTON010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34979_length_2373_cov_2.618205"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="P5292_08860"
     CDS             <1..923
                     /locus_tag="P5292_08860"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRS39274.1"
                     /translation="PFKGNVDLAKLDALVKQHGAKKIPYVSLATSVNMAGGQPVSMQN
                     LKELYRYTRKHGIRIIHDMTRVAENAWFIKQREKGYAKKSVAEIVREICSYTDGATMS
                     AKKDALVNIGGFLAVNDRKIYQEASNLVVVYEGLHTYGGLAGRDMEAIAIGITESVDD
                     YHIHARIGQVEYLGNKLRDAGVPIVLPIGGHGIFLDARRFLPHLKQLEFPAQALAAAI
                     YLDSGVRSMERGIVSAGRNKETGDHYYPPLELVRLTIPRRVYTQAHMDVVAESIERVY
                     RRRRSVPGLKMIYEPKYLRFFQARFKPFSK"
     gene            920..2173
                     /locus_tag="P5292_08865"
     CDS             920..2173
                     /locus_tag="P5292_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HRS39275.1"
                     /translation="MSSDHRFRPETLMMSYGYKPELSEGAIKCPIFQTSTFVFKNAAA
                     GKHFFEVAYGIKEKGPKEELGLIYSRLNNPDLEILENRLTLWDEAEEGAVYESGLAAI
                     TTVMLEFLSPGDLLLFSTPLYGGTDHFIHQVLSRFGIMAVPFHAGQGETELAKMIDSS
                     GRSDRLGMIYLETPANPTNALIDIEMCAKVAARYRKLDRPVPVVVDNTYMGPLWQHPL
                     KFGADLVVYSATKYIGGHSDVIAGACLGRKSLMARVRSLRTFLGNMAGPWTGWLLMRS
                     LETLKPRMETQARNAAQVAEFLQAHPKVEKVYYLGLLDSNDPQFAIYKKQCNSAGAMV
                     SFDIRGGEPEAFRFLDNLKLIRLAVSLGGTESLAEHPASMTHCDIQPDARVGMGITDK
                     LIRLSIGVEYAQDIIWDIDQALEKV"
     gene            2229..>2373
                     /locus_tag="P5292_08870"
     CDS             2229..>2373
                     /locus_tag="P5292_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HRS39276.1"
                     /translation="MNFKTIIEPFRIKSVEPISMTTREDRVRYLHEAHYNPFLLSSEQ
                     VLID"
BASE COUNT          577 a          628 c          659 g          509 t
ORIGIN      
        1 ttcctttcaa aggaaacgtc gacttggcaa agctggatgc acttgtaaag cagcatgggg
       61 cgaaaaagat tccctacgtc tccttggcga ccagtgtgaa catggccggc ggacaaccgg
      121 tttccatgca gaatctgaag gagctttatc gatatacgcg taagcacggt atccggatca
      181 tccacgacat gacccgggtg gccgaaaatg cctggttcat taagcaaaga gaaaaaggat
      241 acgcgaagaa aagcgttgca gagatcgttc gggaaatctg ttcctatacc gatggggcca
      301 cgatgagcgc caagaaagac gcgctcgtca atatcggcgg gttcctggcg gtgaacgacc
      361 gcaagatcta ccaggaagcc agtaaccttg ttgtagtgta tgaaggcttg cacacctatg
      421 gcggactcgc gggacgcgac atggaggcga ttgcgatcgg cattaccgag tcggtcgacg
      481 attaccatat ccacgcccgg ataggccagg tggaatacct gggcaataaa ctgcgcgatg
      541 ccggagtgcc gatcgtattg ccgatcggtg gtcacggcat ctttcttgac gcccgccggt
      601 tcctgccaca cctgaaacaa ctggagttcc ccgcacaggc gctggccgcg gcgatctatc
      661 ttgattcggg tgtgcgatcg atggaacgcg gtatcgtatc cgccggacgc aacaaggaaa
      721 cgggcgatca ctactatccg ccattggagt tggttcgtct gactatcccg cgtcgggtct
      781 atacgcaggc ccatatggac gttgtcgccg aatccatcga acgtgtctat cgccggcgcc
      841 gtagtgttcc cggcttaaaa atgatctatg agccgaagta tctgcggttc tttcaagcca
      901 ggttcaaacc attctcaaaa tgagtagcga tcatcgattc cggccggaga cgttgatgat
      961 gtcttacggc tacaagcctg aactatcgga aggcgctatc aagtgcccga tcttccagac
     1021 ctccaccttc gttttcaaga acgcggccgc gggcaagcat tttttcgaag tggcatatgg
     1081 aatcaaggag aaagggccca aagaagaact ggggctcatc tacagccggc tgaacaatcc
     1141 cgacctggag attctcgaaa atcgactcac cctgtgggat gaagcggagg agggtgcggt
     1201 ctacgagagc ggtcttgccg ctatcaccac ggtcatgctg gaattccttt ctccaggcga
     1261 cctgctgctt ttcagtacac cgctctacgg tggtaccgac catttcatcc atcaggtgct
     1321 gagccggttt ggtatcatgg ccgttccgtt ccatgcgggc cagggagaaa cggaactcgc
     1381 gaaaatgatc gattccagtg gcaggtcgga tcggctcggg atgatttacc tcgagacacc
     1441 ggccaatcct accaacgcgc tcatcgatat cgaaatgtgc gcgaaggtgg ctgcccgcta
     1501 tcggaagctt gatcgtcctg tgcccgttgt ggtcgataat acttatatgg gtcccttgtg
     1561 gcagcacccg ttgaaattcg gagcggacct ggtcgtgtat tcggcaacca agtatatcgg
     1621 cggccattcc gatgtgattg caggcgcatg tcttggacgg aaatcgttga tggcccgcgt
     1681 tcgttcgctg cgtacgtttc tcgggaacat ggccgggccc tggacaggct ggttgctcat
     1741 gcgaagcctc gagaccctga aaccacgtat ggaaacacaa gccaggaacg cggcgcaggt
     1801 ggccgaattt ctgcaggccc acccgaaggt ggaaaaagta tactacctcg gattgctgga
     1861 ctcgaacgac cctcagttcg ccatctacaa aaagcaatgc aattccgccg gagccatggt
     1921 ctccttcgat atacgcggag gagagccgga agcgtttcgg ttcctcgaca acctgaagct
     1981 gatccggctg gctgtcagcc tgggcggcac ggaaagcctc gcggaacacc ctgcaagcat
     2041 gacgcattgc gacattcaac ccgatgcaag ggttggaatg ggcattacgg ataagttgat
     2101 ccgactttcg atcggcgtgg agtatgcaca ggatatcatc tgggatatcg accaggcact
     2161 tgaaaaagtt taaagatcag actaccgaaa gaaaataata gcattcacac gattaaagta
     2221 gaagaccggt gaactttaaa accatcatag agcctttccg catcaaaagc gtcgagccga
     2281 tttccatgac aacccgtgag gatcgggtac gctacctgca cgaagcacat tacaacccct
     2341 tcctgctgtc gagtgaacag gtcttaatcg act
//
LOCUS       DAOTON010000443         2371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_35013_length_2371_cov_2.823402, whole genome shotgun sequence.
ACCESSION   DAOTON010000443 DAOTON010000000
VERSION     DAOTON010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35013_length_2371_cov_2.823402"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..489
                     /locus_tag="P5292_08875"
     CDS             10..489
                     /locus_tag="P5292_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRS39277.1"
                     /translation="MPEQQHNPFMKEAIALSRNGSSTNQGGPFGCVIVKDGKIVGRGS
                     NSVTSTNDPTAHAEVVAIRDACRSLGTFQLTGCDIYTSCEPCPMCLGAIYWARPDRVF
                     YANTRHEAAEAGFDDAFIYEEINVPPTERKITMIHTPDPDAMQVFRDWIIKPDKTPY"
     gene            511..1353
                     /locus_tag="P5292_08880"
     CDS             511..1353
                     /locus_tag="P5292_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRS39278.1"
                     /translation="MKWLGRRESGNVEDRRGMAGPVAVGGGLIGLVILLINAFMGGDT
                     GELLNQLQQQAAPTEQRATSPEEDQLAKFASVVLADNEDVWTKIFRENGATYDAPKMV
                     LFSNATQSGCGNASSSVGPFYCPADNKVYLDLAFFEELKNRLGAGGDFAIAYVIAHEV
                     GHHVQNLMGTSDKVRQLQSRTDEKGANRLSVALELQADFYAGLWTHYNQQMNNVLEPG
                     DIEEALGAASAVGDDRLQKRAQGYIVPDAFTHGTSEQRMYWFKRGYESGDLKAGNTFE
                     EVLN"
     gene            complement(1359..2327)
                     /gene="moaA"
                     /locus_tag="P5292_08885"
     CDS             complement(1359..2327)
                     /gene="moaA"
                     /locus_tag="P5292_08885"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRS39279.1"
                     /translation="MLTDTHNRKHDYLRISLTDACNIRCTYCMPEHAVFAAREHLMRS
                     DEIIELASCFVRLGVRKIRLTGGEPLVRPDLSAILRGLTLLRPAGLEELTMTTNGIRL
                     HEVIDELENAGMRSVNVSLDTLMPERFREITRRDHFHRVLSNIHLLLDRSFRVKVNVV
                     VMNGVNDDEVVDFVTMTRDYPLHVRFIEYMPFEGNRWTDRRLISSKELLERVSQFYSV
                     EPLERNAHDTSRAFKVMGYSGTFAFISTMSTPFCGDCNRLRLTADGKMKNCLFSKGET
                     DLLSALRNGTPVEVLIRENLSGKAAQWGGQDLYGPTQNRPMVSIGG"
BASE COUNT          566 a          712 c          608 g          485 t
ORIGIN      
        1 ttcctgacta tgcctgaaca gcagcacaac ccattcatga aggaagcgat cgcgctctcc
       61 cggaacggtt cctccaccaa ccagggtggg cctttcggtt gtgtgatcgt caaggacgga
      121 aagatcgtcg gccgtggctc caacagcgtg acttcgacca acgaccccac cgcccacgcc
      181 gaagtggttg ccattcgcga tgcctgccgc agcctgggca ccttccaact caccggctgt
      241 gacatctaca ccagctgcga accctgcccc atgtgcctcg gcgccatcta ctgggcgcga
      301 cccgaccggg tcttttatgc gaatacccgc cacgaagccg cggaagcggg tttcgacgac
      361 gcgttcattt acgaagagat caacgtacca cccaccgaac gcaagatcac aatgatccat
      421 acccccgatc ccgacgccat gcaggtgttt cgcgattgga tcatcaaacc cgataagact
      481 ccatattaaa ccatctaatc agctaccacc atgaaatggc tcggaagaag ggaaagcgga
      541 aacgttgaag accgtcgcgg catggccgga ccggtcgccg tgggcggcgg actcatcgga
      601 ctggtgatcc tgctgatcaa cgccttcatg ggcggtgaca cgggagaact gctcaaccaa
      661 ttgcaacaac aggctgctcc gaccgaacaa cgcgccacca gcccggagga agatcaactc
      721 gccaagttcg cttcagtggt ccttgctgat aacgaggatg tctggacgaa aatcttccgg
      781 gaaaacggcg ctacctacga cgcgccgaag atggtgctgt tctccaacgc gacacaatca
      841 ggttgcggca acgcgagttc atccgtaggc cctttctatt gcccggccga caataaggtt
      901 tacctcgatc tcgcgttctt cgaggaattg aaaaaccgcc tcggtgccgg cggcgacttc
      961 gcaatagcct atgtgatcgc acacgaagta ggtcatcatg tccaaaacct gatgggcact
     1021 tccgataagg ttcggcaact ccaatccagg accgacgaaa aaggcgctaa ccgcctctcc
     1081 gttgccctcg aattgcaagc cgatttctac gctggcctgt ggacgcacta caaccagcag
     1141 atgaacaacg tacttgaacc aggcgatatc gaagaagcat tgggtgccgc ttccgccgta
     1201 ggcgacgacc ggctgcagaa gcgggcacaa ggctatatcg ttcctgatgc gttcacccat
     1261 ggaacttccg agcaacggat gtactggttt aaacgcggct atgagtccgg cgacctgaaa
     1321 gctggtaata cattcgaaga agttcttaat tgattatttc aaccaccgat cgataccatc
     1381 ggtcggtttt gcgtcggtcc gtacaggtcc tgtccgcccc attgcgcggc ttttcccgag
     1441 aggttttcac ggatcaacac ttcgaccggt gttccatttc gtaaagcgga aagcagatcg
     1501 gtctcccctt tcgagaaaag acagttcttc attttaccgt cggcggtcag ccgcagccgg
     1561 ttacaatccc cgcaaaacgg cgtactcatc gtactgatga aggcaaaagt gccgctgtag
     1621 cccatgactt tgaaagcacg ggatgtatcg tgggcattac gttccagggg ctccacggaa
     1681 tagaactgcg aaacacgttc cagtaactcc ttgctgctga tcagtcgccg gtcggtccag
     1741 cgattcccct caaacggcat gtactcaatg aatcgcacat gcaacgggta atcccgggtc
     1801 atggtaacga agtccacgac ctcatcgtca ttcacaccgt tcatgacaac cacattcact
     1861 ttcacacgaa agctgcgatc aagaagcaaa tggatgttcg acaacacgcg atgaaaatga
     1921 tcccgacggg tgatttcccg gaatcgctcc ggcatcaggg tgtccagact gacattcacc
     1981 gaacgcatac cggcattctc caattcgtct atcacttcgt gcaggcgaat gccgttcgtt
     2041 gtcatcgtca gctcttccag acctgctggc cgcagcaaag ttaggccccg tagaatggca
     2101 ctgagatccg gacgcaccaa cggctctcct ccggtcagtc ggatcttgcg cacacctaat
     2161 cggacaaagc aggatgccaa ctcgatgatc tcatccgaac gcatcaaatg ctcccgcgcg
     2221 gcgaacacgg catgctccgg catacaatac gtgcaacgga tgttgcaggc atcggtcagc
     2281 gagatgcgca gatagtcgtg cttgcggtta tgcgtatcgg tcagcatcgc ggctcaggac
     2341 ttggctctcg cctgttcagg ctcgtgggac a
//
LOCUS       DAOTON010000444         2370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_35026_length_2370_cov_3.697624, whole genome shotgun sequence.
ACCESSION   DAOTON010000444 DAOTON010000000
VERSION     DAOTON010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35026_length_2370_cov_3.697624"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /gene="ftsZ"
                     /locus_tag="P5292_08890"
     CDS             complement(<1..1086)
                     /gene="ftsZ"
                     /locus_tag="P5292_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRS39280.1"
                     /translation="MEFDLLRDQPSIIKVIGVGGGGSNAVNHMYRQGIKGVEFIICNT
                     DAQALEMSPVPNKIQLGLSLTDGRGAGSIPEVGRNAAIESIEDIRRLLLNNAKMVFIT
                     AGMGGGTGTGAAPIIAQAAREMGILTVAIVTIPFEFEGKKRKQQADDGIEQLKKSVDS
                     ILIIRNQKLREMFGNLNLSEAFANADNILTTAAKGIAEIITVTGYINVDFEDVKTAVQ
                     NSGVAIMGSAIAEGDNRAVKAVEQALSSPLLNDNNIKGARYILMNIASGSKEVTMDEI
                     GDITDYIQDQAGLTADIIWGNCHEESLGEQLSVTIIATGFKTRAEQGVETEREHKSAK
                     TVHVLDQPLTADAVPAETPVVDSMEPVL"
     gene            complement(1133..>2370)
                     /gene="ftsA"
                     /locus_tag="P5292_08895"
     CDS             complement(1133..>2370)
                     /gene="ftsA"
                     /locus_tag="P5292_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008637262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRS39281.1"
                     /translation="SESLGVTRGVVTNIVNTIASIKKSIEEASGRSDVDIHNVFVGIA
                     GQHIKSLQHHGMRTRSSSDDEITQADIDAIVADMYKLAMPPGEEIIHVIPQEYIVDNE
                     IGIKNPIGMSGVRLEANCHIITGQVTAAKNIYKCVKRAGLTTEGLFLEPLASSEAVLT
                     EEEKEAGVVLVDIGGGTTDIAIFQDGVIRHTAVIPFGGNVITEDIKEGCAILKNQAEL
                     LKVKFGSALADKNQENEIVSIPGLRGREPKEISLKNLASIIQARMEEIIELVYQEIRS
                     SGYEKKLIGGIVITGGGAQLKHIVQLVEYLTGMDTRIGYPNEHLAKGADELKSPMYAT
                     GVGLVIKGFHELERKRQSQLASQQTVTETGARNMAGKSAKEDKARVAQPTEDNNKRGF
                     IKSILEKIFDEDDSDQPLT"
BASE COUNT          456 a          665 c          647 g          602 t
ORIGIN      
        1 caaaaccggc tccatggaat cgacaaccgg agtctctgca ggtacggcat cagcggtcag
       61 gggctggtcg aggacatgaa cggtcttggc cgacttgtgc tcgcgctcgg tttcgacacc
      121 ttgctctgcc cgggtcttga aaccggtggc aataatcgta acactcagtt gctcgcccag
      181 gctttcttca tggcaatttc cccaaatgat gtcggcggta aggccggcct gatcctggat
      241 gtaatcggtg atatcgccga tctcgtccat ggtcacctct ttggaaccgg atgcgatgtt
      301 catgaggatg taacgagcac ccttgatgtt gttgtcgttc agcaaaggcg aggaaagcgc
      361 ctgctcgact gccttgacgg cacggttatc accttccgcg atggcggaac ccatgatcgc
      421 aacaccggaa ttctgtacgg cggtcttcac gtcttcgaaa tcgacgttga tgtatcccgt
      481 cacggtgatg atctccgcga tgcctttcgc cgcggtggtg agaatgttat cagcgttcgc
      541 gaatgcttcg gaaaggttga ggttgccgaa catttcacgg agtttctggt tgcggatgat
      601 gaggatggaa tcgacactct tcttgagctg ctcgataccg tcatcagctt gttgtttccg
      661 cttcttgcct tcgaactcga acggaatggt gacgatcgcg actgtcagga tccccatctc
      721 gcgtgctgcc tgcgcgatga tcggcgcggc gcccgttccg gttcctccgc ccatgcccgc
      781 ggtgatgaaa accatcttcg cgttattgag caacagtcga cggatatcct cgatactttc
      841 gatcgccgca ttacggccaa cctccggaat cgaaccggca ccgcggccgt ccgtcagact
      901 tagtcccagt tggatcttat tgggaacggg agacatttcc agcgcttgtg catcggtatt
      961 gcagatgatg aactccacgc ccttgatgcc ctggcgatac atgtggttga cggcattgct
     1021 gccgccgccg ccgacaccga ttactttgat gatggagggc tggtcacgaa gcagatcgaa
     1081 ttccatatgg tgtgtttgtt aaggtcgttg atgtactata tataaagagg tatcaggtaa
     1141 ggggctggtc ggaatcgtcc tcgtcgaaga tcttctcaag aatggacttg atgaatccgc
     1201 gcttgttgtt gtcttcggtc ggctgtgcca ctcttgcttt atcttccttg gccgacttgc
     1261 cggccatgtt cctggcaccc gtttcggtaa cagtttgctg gctggccaac tgcgactggc
     1321 gcttgcgctc cagttcgtgg aaacctttga tcacgagacc aacgccggta gcgtacatcg
     1381 ggctcttcag ttcgtcggcg cctttggcca ggtgttcatt cgggtagccg atgcgggtat
     1441 ccatgccggt caggtattca accaactgca cgatatgctt gagttgggca ccgccaccag
     1501 tgatcacgat cccgccgatg agtttctttt cgtaaccgga actgcggatc tcctggtata
     1561 ccagctcgat gatctcttcc atccgggcct ggatgatact tgccaggttc ttcagggaga
     1621 tctccttggg ctcgcggccg cgcagtccgg gaatcgatac gatctcgttt tcctggttct
     1681 tatcggccag tgccgaaccg aatttcacct tcaacagttc agcctgattc ttcaggatcg
     1741 cgcaaccctc cttgatatct tcggtaatga cattgccccc gaacgggatc acggcagtat
     1801 gccggatgac gccatcctgg aagatggcga tatcggtcgt accgccgccg atgtcgacga
     1861 gcactacgcc ggcttccttc tcttcctcag tcaaaaccgc ttccgaggag gccaacggct
     1921 ccagaaagag gccctccgtc gtgaggccgg cacgctttac acacttgtag atgtttttcg
     1981 cagcggttac ctgcccggta atgatgtggc agttcgcttc caaacgcaca cccgacattc
     2041 cgataggatt cttgatcccg atctcgttgt ccacgatgta ctcctgtgga atgacgtgga
     2101 tgatctcctc acccggcggc atggccagtt tgtacatgtc ggctacgatg gcatcgatgt
     2161 ccgcctgcgt gatctcgtcg tccgaactgg agcgcgtccg catgccatgg tgttggaggc
     2221 ttttgatatg ttgaccagca ataccgacga atacgttatg gatatccaca tccgaacggc
     2281 cggaagcttc ctcaatggat ttcttgatcg aagcgatggt attgacgatg ttcgtcacca
     2341 ccccgcgggt cacgccaagc gactcggact
//
LOCUS       DAOTON010000445         2366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_35095_length_2366_cov_3.894851, whole genome shotgun sequence.
ACCESSION   DAOTON010000445 DAOTON010000000
VERSION     DAOTON010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35095_length_2366_cov_3.894851"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1646
                     /locus_tag="P5292_08900"
     CDS             108..1646
                     /locus_tag="P5292_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRS39282.1"
                     /translation="MATTEEFKQDIAAGYGFQQKSILLGAAMLDGKAVPGTQVRIPLA
                     TVNRHGLIAGATGTGKTKTIQQFAESLSEQGVNVLLMDIKGDLSGIAQPGTSNAKIEE
                     RHQLIGQAWQPFAAPVELFSISEEKGVRLRATVSEFGPVLFSKILELNENQQGVVTIV
                     FSYCDDNRLPLLDIKDFREALRFLTEEGKEKVKQYGQISTTSAGVIFRKLLEVEQQGA
                     DRFFGERSFDVEDFLSDDPAKGRINVLRLCDIQDKPKLFSTFMLCLLAEIYQKFPEMG
                     DKAAPKLAIFIDEAHLVFRDATKTLMDQLETVVKLIRSKGVGLYFCTQVPSDIPDIIL
                     SQLGAKFQHALRAFTAKDRKDIKLVAQNYPETNYYQTEELLTGLGIGEALVTVLNEKG
                     IPTPLVHTLLCAPRSRMDVLTPSEQDEIVRRSPLAGAYNESVDRESAYEILKGKFDRQ
                     ADQESAAEDQPAQQKKVEKESAGGGILDSIVSATKNPLVKTVARELTRGLLGAFLGKP
                     SRRR"
     gene            1656..2078
                     /locus_tag="P5292_08905"
     CDS             1656..2078
                     /locus_tag="P5292_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRS39283.1"
                     /translation="MSDKPLAERVVDRMYDHDAFSQWLGIERLEVATGRCVLRMTIRQ
                     EMTNGFAIAHGGITYSLADSALAFASNGHGRRAVSVETSISHTVALRTGDVITATAEE
                     VSLSDKIGVYYITITRQDGKTVALFKGTVYRTSHDWDV"
     gene            2096..>2366
                     /locus_tag="P5292_08910"
     CDS             2096..>2366
                     /locus_tag="P5292_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226678.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HRS39284.1"
                     /translation="MQQAYIVDSIRTPIGSFAGTLSSVRADDLAVIVLQELLKRNPSA
                     DPAMIGDVIMGCANQAGEDNRNVARMALLMAGYPVTVPGETVNRLC"
BASE COUNT          552 a          660 c          664 g          490 t
ORIGIN      
        1 gcgcggacta cgggaaactt acgaggagga tcgttaccgc ccgagtgttt tgttaaagca
       61 aatggccaca tccggtcaga aattccacgc ataattactc cggcgctatg gcaacaacgg
      121 aagagttcaa acaggatatt gctgccggct atggatttca gcaaaagagc atcctgctgg
      181 gtgccgcgat gctggatggc aaggcagttc cgggtacgca ggtacgtatt cccctggcta
      241 cggtcaaccg tcatggtttg atcgccgggg caaccggtac gggaaagacc aagacgatcc
      301 agcaattcgc ggaaagtctg tcggagcagg gcgtcaatgt cctgctcatg gacatcaaag
      361 gcgacctcag cggcattgcg caaccgggca cttccaatgc gaagatcgaa gaacgtcatc
      421 aactgatcgg tcaggcctgg caaccctttg ctgcaccggt agaactgttt tccatttcgg
      481 aagaaaaggg tgtccggctg cgggcgaccg tctcggaatt cggccccgtc ctgttctcca
      541 agatcctcga gttgaacgag aatcaacagg gcgtcgtgac catcgtattt tcctattgtg
      601 atgacaaccg tttgcccttg ctcgatatca aggactttcg cgaagccttg cgcttcctga
      661 cagaggaggg taaggagaaa gtcaaacaat acggacaaat cagtaccacg agcgcgggtg
      721 tgatcttccg aaaactactc gaggtcgaac agcagggcgc cgaccgcttt tttggagaac
      781 gatcgttcga tgtggaggat ttcctgtcgg atgatcctgc gaagggacgg atcaatgtgc
      841 ttcgcttgtg cgatatccag gacaagccca agctcttctc gactttcatg ctctgcctgc
      901 tggcggagat ctatcagaag tttccggaga tgggcgataa ggccgctccg aagctggcca
      961 tcttcatcga cgaagcgcac ctcgttttcc gcgacgcgac caagacgctg atggaccagt
     1021 tggaaacggt cgtcaagctg atccgctcca aaggtgtcgg cttgtacttc tgcacgcagg
     1081 tgccttccga tattcccgat atcatcctga gtcagctcgg tgcgaagttc cagcatgcgc
     1141 ttcgtgcttt cacggcaaaa gaccggaaag acatcaaact cgttgcgcag aactatcccg
     1201 aaacgaacta ctatcagacc gaagaactgc tcaccggcct cggaatcggg gaagcgctcg
     1261 tcacggtgct gaatgagaag ggtattccta cgccactcgt tcataccctg ctctgcgcac
     1321 cgcgttcccg catggacgtg ctcacgcctt ccgaacagga cgagatcgtg cgtcgatcac
     1381 cgcttgcggg tgcttacaat gaatcagtcg atcgcgagag cgcctatgaa atcctgaagg
     1441 gaaagttcga ccgtcaggct gatcaggaaa gtgctgccga agatcaaccg gcacagcaga
     1501 aaaaagtaga aaaagaatcg gccggcggcg ggatcctcga cagcatcgtc tcggcgacga
     1561 agaatccgct tgtcaaaaca gttgcacggg aactgacccg aggtttgctc ggcgccttcc
     1621 ttggaaaacc cagccgacgc agataattta gcccaatgtc cgataaacct cttgccgaac
     1681 gggtcgttga ccgcatgtat gaccacgatg cgttcagcca atggctcggc atcgaacgtc
     1741 tggaagtcgc caccggccga tgcgtgctgc gcatgaccat ccgccaggag atgaccaacg
     1801 gattcgcgat cgcccacggc gggatcacct attcactggc cgacagcgcc ctggcctttg
     1861 cgtcgaatgg tcatggacgc agggcggtct cggttgagac ttcgatctcg catacggttg
     1921 ccctccgtac aggcgatgtt attaccgcga ccgcggaaga ggtgtctttg agcgacaaga
     1981 tcggcgtgta ttatatcacc atcacccggc aggacggaaa gaccgttgcc ctcttcaagg
     2041 gaaccgttta ccgcacgagc cacgactggg atgtctgatc ccgaacaacc tgattatgca
     2101 gcaagcttat atcgttgaca gtatccggac gccgatcggc agttttgcgg gaacgctgtc
     2161 atccgtacgt gccgacgacc tggcggtgat tgtcttacag gaactcctca aacgaaaccc
     2221 ctcggccgat cccgcgatga tcggtgatgt gatcatgggc tgtgccaacc aggccggcga
     2281 agacaaccgg aacgtagccc gtatggcctt gctcatggca ggttatccgg tcaccgtgcc
     2341 tggtgaaacc gtgaaccggc tttgcg
//
LOCUS       DAOTON010000446         2365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_35122_length_2365_cov_3.755844, whole genome shotgun sequence.
ACCESSION   DAOTON010000446 DAOTON010000000
VERSION     DAOTON010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35122_length_2365_cov_3.755844"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="P5292_08915"
     CDS             <1..571
                     /locus_tag="P5292_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HRS39285.1"
                     /translation="LTPKSEIEGEMGESKMGLQARLMSQALRKLTATISRTGCCCIFI
                     NQLREKIGVMFGNPETTTGGNALKFYASVRLDIRRIGQIKDGETVVGNRTRVKIVKNK
                     LAPPFRTAEFDIVYGEGISKMGEIVDLGVDKGVIRKSGSWFSYGETKLAQGRDAVKQL
                     LLDNPELAEELEGKIRAAISSTEMPVEVE"
     gene            849..1847
                     /locus_tag="P5292_08920"
     CDS             849..1847
                     /locus_tag="P5292_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS39286.1"
                     /translation="MTPAGCRQDSGSGQTDTLSTQDTSQSAIDRRFRELNEQLLRDSL
                     NPGLLFERAQLMKIRGNIPGAYQEMERVVTLDSGKADYFLALADIAFRALQVPRSIEA
                     FERCLRIDPKNTEAHLRLAELYFYLKAYPKAIESANNALRLNDRLARAYSIKGFVYKE
                     SGDTAKAISSFQTVLELDPENYDTQRELGRIFAAKKDPLALQYYNNALRIRPGSGEIL
                     YNRGLYYQEIGQLKEAEADYATLLASDPKDPDAHHNLGYIALRYKEDYPAAIRHFTDA
                     IRYNPEYAEAYYHRGLAEERMGNRTAALTDYRKATTIYPSFALAKDGIKRVAGRGE"
     gene            complement(2074..>2365)
                     /gene="rplT"
                     /locus_tag="P5292_08925"
     CDS             complement(2074..>2365)
                     /gene="rplT"
                     /locus_tag="P5292_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRS39287.1"
                     /translation="KLAKGYFGSRGNVWTVARNTIEKGLGYAYRDRRAKKRDFRALWI
                     QRINAGAREHGMSYSELMGKIHAKGLEINRKSLADLAMNHPAAFKAVVDSVK"
BASE COUNT          560 a          612 c          650 g          543 t
ORIGIN      
        1 cctgacgccg aagagcgaga tcgaaggcga aatgggagag agcaagatgg gcctgcaggc
       61 ccgcctcatg tcgcaggcgc tccgtaaact cacagctact atctcccgta ccggttgctg
      121 ctgcatcttc atcaaccagc tgcgcgaaaa gatcggcgtc atgttcggca atcccgagac
      181 gaccaccggc ggtaacgcgc tcaaattcta cgcttctgta cgtctcgaca tccgccgcat
      241 cggccagatc aaggacggag aaacggttgt cggtaaccgc acccgtgtga agatcgtgaa
      301 gaacaaactg gcgcctcctt tccgtacggc tgagttcgac atcgtgtacg gggaaggaat
      361 ttcaaagatg ggcgagatcg tggatctcgg cgtcgacaag ggtgtcattc gcaagtccgg
      421 atcctggttc agttacggcg agaccaaact tgcccagggc cgcgatgccg tgaaacagtt
      481 gctcctcgac aacccggaac tcgccgagga actcgaaggc aaaatccgcg cagccatcag
      541 cagcaccgaa atgccggtgg aggtggagta agcaggcagg aaaagattcc gatgcgcaaa
      601 tcctcaatca ctaatcacaa ataacttgta acttgtaact tgtaactagt aactagtaac
      661 tagtaattag tgattagtga ttagtgatta gtgatttggg gaaggatttt tgagcgtatc
      721 gcttcttcga ttcttccttt cccaatttcc ttacttttat accgccgggt tgattgtccc
      781 ggcggtattt ttttatgtta aacaggattg taagtcatct cctgaccgcg ttggttatcg
      841 cgttcctcat gacacctgct ggttgccggc aggattcggg aagcggtcag accgataccc
      901 tttcgactca ggatacgagc cagtcagcca tcgatcgacg gtttcgggag ttgaacgaac
      961 agttgttacg tgattcgttg aatcccggac tcctgtttga gcgcgctcag ttgatgaaga
     1021 tcaggggcaa tattccgggc gcttaccagg aaatggagcg ggtagtgacc ttggattcgg
     1081 gcaaggcgga ctatttcctg gcgctggctg atatcgcatt ccgtgcatta caggtgccgc
     1141 gttccattga agcattcgaa cgctgcctga ggatcgaccc gaagaatacc gaagcgcact
     1201 tgcgcctggc cgagttgtat ttctacctga aggcttatcc caaggcaatt gaatcggcga
     1261 acaacgcgct tcgattgaac gaccggctgg cgcgcgcgta ttcgatcaaa ggattcgtct
     1321 acaaggagtc gggcgatacg gccaaagcga tcagcagttt ccagaccgtg ctcgaactgg
     1381 atcccgagaa ctatgacacc caacgcgaac tgggccgcat cttcgcggca aaaaaggatc
     1441 ccctggcgtt gcagtattat aacaatgctt tacgtatacg tccgggcagc ggtgagatcc
     1501 tgtacaaccg tggtttgtac tatcaggaga tcggacaact gaaagaagcc gaggccgatt
     1561 acgcgacgct cctggcaagc gatcccaaag atccggatgc ccatcacaac ctcggataca
     1621 tcgcgctgcg ttacaaggaa gactatccgg ccgccatccg gcatttcacc gatgcgatcc
     1681 gctataaccc ggaatacgcg gaagcctatt accaccgcgg cttggcggag gagcgcatgg
     1741 gcaacagaac cgcagcactg accgactacc gaaaagccac caccatttat ccgtcgtttg
     1801 cgttggctaa ggatgggata aagcgggtgg ctggaagggg agagtagtgg cgggagggat
     1861 tggtaattgg taactagaaa ctagaaacta gaaactagaa actagtaatt agtaattagt
     1921 aattcgtgat tggttgggag cgtttaaggg atgttttttg ttttacttat cctcgtccct
     1981 cgtccctcgt ccctcgtccc tcgtcccttc aaatgcctcc caacaaaaaa ggccccgtat
     2041 ccggagcctt ttcgtttttc aggcaaagcg atcttacttc accgaatcca caacagcctt
     2101 gaaggctgcc gggtgattca tggcgagatc ggcgagggac ttgcggttga tctcaagtcc
     2161 tttggcgtgg atttttccca tgagttcgga atagctcatt ccgtgttcgc gggcgccggc
     2221 gttgatacgc tggatccaaa gggcgcggaa gtcgcgcttc ttggcgcggc ggtcacggta
     2281 agcgtagccg aggcccttct cgatggtgtt acgggcgacg gtccaaacat ttcctctcga
     2341 tccgaaataa cctttggcga gtttg
//
LOCUS       DAOTON010000447         2365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_35127_length_2365_cov_2.761472, whole genome shotgun sequence.
ACCESSION   DAOTON010000447 DAOTON010000000
VERSION     DAOTON010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35127_length_2365_cov_2.761472"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..868)
                     /locus_tag="P5292_08930"
     CDS             complement(137..868)
                     /locus_tag="P5292_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRS39288.1"
                     /translation="MENNWNSPFGQPAAPASQAVSRSFISGVFSWMGIALAISAITAY
                     VFGTDASYMSYLINTERGGLSVLGYIVMFAPIGLVLLMGFGINRLSVPALTGVFLVYS
                     VLMGMSLSFIFLAYTQSVIYQVFFISAAMFGVMALLGYTTKTDLTKLGSLLFMALIGI
                     VIASLVNMFLRSPGFSYVISFIAVIVFTGLTAYDVQKLKQIGTQTEAGTEATAKLSIM
                     GALTLYLDFINLFLALLRIFGGRRD"
     gene            complement(964..1599)
                     /gene="folE"
                     /locus_tag="P5292_08935"
     CDS             complement(964..1599)
                     /gene="folE"
                     /locus_tag="P5292_08935"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRS39289.1"
                     /translation="MPIDSHMHEENEMDGYTKIDQYNPVLVKQIASDYEDIIRRIGEQ
                     PERDGLAKTPERAAKAMQFLTHGYDLDPAGILRSALFKDEYQQMVIVKDIELYSLCEH
                     HLLPFFGKAHIAYIPNGHIVGLSKIPRVVDAFARRLQVQERLTTQIRDCIQETLNPLG
                     VAVVIEARHLCMLMRGIQKQNSVATTSAFTGAFENEKTRSEFIRLIAANLS"
     gene            complement(1611..2021)
                     /locus_tag="P5292_08940"
     CDS             complement(1611..2021)
                     /locus_tag="P5292_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRS39290.1"
                     /translation="MIYITRKETFCAAHKLSRPDWSLEKNLEIFGKCSNPNWHGHNYT
                     LWITVKGEVNPETGFVANLAEVSNIIRVNVLDKVDHRNLDLDVDFMKGKLSSTEVLAM
                     EIWKQLQQPINALGCSLHCVKLQETEKNSVEYFG"
     gene            2094..>2365
                     /locus_tag="P5292_08945"
     CDS             2094..>2365
                     /locus_tag="P5292_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HRS39291.1"
                     /translation="MRILVVSATQAEAAPLQQLLTEHPVGHDVQYLVTGIGMTATAYA
                     LTRYLLTLPVDLALNIGLAGSFRRDLEPGASVWVKEDRFSDLGAEDG"
BASE COUNT          583 a          597 c          628 g          557 t
ORIGIN      
        1 tttttcataa ggaggaacgc tagtgatcgt tgattcgtct ggattcaaat ttaacaaatg
       61 caccccaaac cactcccgaa aagcggacaa aaaaaagagg ccggataacc ggcctcttta
      121 ataatttcaa gtgaaatcag tcgcggcgac cgccgaagat gcggagcaag gcgaggaaga
      181 ggttgatgaa gtcaaggtag agtgtcaacg ctcccatgat gctcagtttg gctgttgctt
      241 ccgtaccggc ttccgtctgg gtaccgatct gcttcaactt ttgaacgtca taggcagtca
      301 gaccggtgaa gacgatcacc gcaatgaaac tgatcacgta gctgaaaccc ggactgcgca
      361 ggaacatgtt caccagggaa gcgatcacga taccgatcag ggccatgaac aggagcgatc
      421 cgagtttggt cagatctgtt ttcgtggtat aacccaggag ggccattaca ccaaacatgg
      481 cggcactgat gaaaaagacc tgatagatga ccgattgcgt gtatgccaag aagatgaaac
      541 taagactcat gcccattagc accgaataga ccaggaacac cccggtcagg gcaggtacgg
      601 acaggcggtt gataccgaaa cccatgagta agacgagtcc gatcggagcg aacatcacga
      661 tatagccaag gaccgaaaga cctccgcgtt cggtgttgat gagatagctc atgtacgacg
      721 cgtccgtgcc gaatacataa gcggtgatag cggagatagc cagtgcaatt cccatccagg
      781 agaaaactcc ggaaatgaaa ctacgggaaa ccgcctgcga tgcaggagcg gccggctggc
      841 caaagggaga attccagtta ttttccattc gttgaagata cggtttatgg caggttaagg
      901 ttgttatgga ttttttaatt gttcagcgcg cactttcatc aaatctcacg ccaaagttcc
      961 cattcatgaa agattggcag cgatcagtcg gatgaactcg gagcgtgttt tttcgttctc
     1021 gaatgcgccg gtaaaagcgg atgtggtggc aacggaattc tgcttttgaa ttccccgcat
     1081 gagcatgcaa aggtgacgag cctcgatgac gaccgcaacg cccagcggat tcagggtttc
     1141 ctgaatacaa tcccggatct gggtcgtcag gcgctcctgc acttgcaggc gacgagcgaa
     1201 ggcatcgacg acccgcggga tcttactcaa ccccacgata tggccattcg gtatgtacgc
     1261 gatgtgcgcc tttccaaaga acggtagcag atgatgctcg cagagcgaat acaactcaat
     1321 atccttcacg atcaccattt gctggtattc gtctttgaaa agcgctgaac ggaggatccc
     1381 ggccggatcc aggtcataac catgggtcag gaactgcatg gccttcgctg cgcgctccgg
     1441 ggtcttggcc agaccgtcgc gttccggttg ctcgccaatc cgacggatga tgtcttcata
     1501 gtcggatgcg atctgcttaa ccaggaccgg gttgtactgg tcgatcttgg tgtagccatc
     1561 catttcattt tcctcgtgca tatgggaatc gatcggcatg ggcgggtcaa ttatccgaaa
     1621 tactcaaccg agttcttttc ggtctcctgt aatttaacac agtgcaacga acagccgagc
     1681 gcattgatcg gttgctgcaa ttgtttccag atctccatcg caagcacttc ggtagagctt
     1741 aacttccctt tcatgaaatc cacatccagg tcgaggttcc gatggtccac tttatccagg
     1801 acattaaccc ggattatgtt gctgacttct gccaaattgg ctacgaaacc tgtttccggg
     1861 ttcacttcac ccttaacagt gatccacaag gtatagttat gtccgtgcca gttcgggttg
     1921 gagcattttc caaatatttc caggttcttt tccagcgacc aatcgggccg tgataactta
     1981 tgggcggcgc aaaaggtctc ttttcgggta atatagatca tgacgggaca aaggtaggaa
     2041 atcatcgggt gggcctcagc gcccggaatc ctgcccgttc gtacctttgt accatgcgaa
     2101 ttttggtcgt ttcggcgacc caggcggaag ccgctccttt gcaacaattg ttgacggaac
     2161 atcccgtcgg tcacgatgtt cagtatctgg ttaccggcat cggcatgact gcaacggcct
     2221 atgccctcac ccggtatttg ctgacgcttc ccgtcgacct tgcgctcaac attgggctgg
     2281 ccgggagctt tcgacgcgac ctggagccgg gcgcaagcgt atgggtcaaa gaagatcgat
     2341 tttctgatct gggagcggaa gatgg
//
LOCUS       DAOTON010000448         2364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_35140_length_2364_cov_3.090082, whole genome shotgun sequence.
ACCESSION   DAOTON010000448 DAOTON010000000
VERSION     DAOTON010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35140_length_2364_cov_3.090082"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="P5292_08950"
     CDS             complement(<1..212)
                     /locus_tag="P5292_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRS39292.1"
                     /translation="MHFELTEEHLMIQKAARDFAQTELKPGVIERDDHQKFPAEQIRK
                     MGELGFLGMMVDPKYGGGGMDTISYVL"
     gene            387..1742
                     /locus_tag="P5292_08955"
     CDS             387..1742
                     /locus_tag="P5292_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39293.1"
                     /translation="MTLKVSALEPTCAAVIRLDPQCEHEGIRAAQLTDGTYLLVGNLV
                     NTATGSGTIAICHLNEYGDTISTRQYGSSGALWTLTDWCATVNGWMLAGVVDSVGNTS
                     GAIWQIDSAGQLITQVRYRMTGERVRFTALVDALEGGWLLAGYRLEIATNRYRFHVRK
                     LDDALQTTWTKDYNVRLGQLLDAEQVPDSSYLFTGYLLDKGDTSGCLFVLHTDLLGDS
                     LWLRKYFGNGWASGASLAQLQSGFVVAGATSVSSVSPSDRFVVRCNEPGDSLWTRSLG
                     TANQERIHDILPVEDDCLLLVGESASQLDVTKFDSSGSLLWSHTHMAGLNDRGKHAFQ
                     TAEGNYAIVGTAGAGSGSSAFYFLVMDTSGAIVLNLVDGFPKPNYLHFYPNPAKGIVR
                     LAPGPVKDLVIFDLLGRVRMRVPEARESLDLGSLLPGCYIVEAENESGAHLQGKLIVA
                     E"
     gene            1739..>2364
                     /locus_tag="P5292_08960"
     CDS             1739..>2364
                     /locus_tag="P5292_08960"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRS39294.1"
                     /translation="MSPETYQVVGLMSGTSLDGLDIALCEFVRGDTWSYRIVEAETVT
                     YPESIASLLRSIDKADAESLVKAQVHYGSYLGEQVRKFLAERSCSADFVSSHGHTIYH
                     QPGNGFTFQLGDGAALSVAAGLPVVSDFRMQDVALGGQGAPLVPVGDQLLFGEFTHCL
                     NLGGIANISFSAGNRRVAGDLCPCNLLLNPVAARRGLPFDRDGQLAQNG"
BASE COUNT          473 a          608 c          697 g          586 t
ORIGIN      
        1 agcacgtagg aaatggtatc cattccaccg ccgccgtact tcgggtccac catcatgccg
       61 aggaaaccga gttcgcccat tttacggatc tgttccgcgg ggaacttctg atggtcgtcg
      121 cgctcgatga cgcccggttt caattctgtc tgggcaaaat cgcgggcagc tttttggatc
      181 atcaggtgtt cttcggtcag ctcgaaatgc atggcagtcg cttgttctta attcgttgtt
      241 tttcggggaa aacgccgcat ttccaaccgg gaaagggcgt attttgtgcg gacaaaccta
      301 cggatttgaa cggtattcta aaaggatgga gaagcgtaac tcgaaggctg gctttcagca
      361 tttgcgtgtc gctcctatgt ccgtttatga ccttgaaagt tagcgcgttg gagccaactt
      421 gtgcggctgt tatccgactc gacccgcagt gtgaacacga gggtattcgg gctgcacaat
      481 tgaccgatgg aacttatctg ctggttggca atctggttaa caccgctacc ggttcgggca
      541 ccatcgccat ttgccatttg aatgaatacg gcgatacgat ttctacacgc caatacggat
      601 caagcggcgc cctctggaca ttgaccgatt ggtgcgcaac ggtcaatggt tggatgctgg
      661 ctggtgtcgt cgatagcgtg ggcaacacct cgggtgcaat ctggcagatc gatagcgcgg
      721 gtcaattgat cacgcaagtc cgttatcgga tgaccggcga acgggttcgg ttcaccgcgc
      781 tggtcgacgc gctggaaggc ggctggttgc tggcgggtta tcggctggaa atcgcgacca
      841 accgttaccg tttccatgtg cgcaaactgg acgacgcctt acaaactacc tggacaaagg
      901 attacaacgt ccgtttagga cagttgctcg atgcggaaca ggttcccgac tcatcctatc
      961 tgtttaccgg gtacctgctc gataagggcg ataccagtgg ctgcctgttt gtgctgcata
     1021 ccgacctgct cggcgattcg ctctggcttc ggaagtattt cggaaatgga tgggcttccg
     1081 gcgcttcgct tgcgcagctg caaagtggtt ttgtcgtggc aggagcgact tccgtttcct
     1141 cggtatctcc atccgatcgc tttgtggtgc ggtgcaacga accgggtgat tcgctctgga
     1201 cgcgatcgct tggaacggcc aaccaggaac gtattcatga catacttccg gttgaagacg
     1261 attgcctgct tttagttggg gaatccgctt cgcaactgga tgtaaccaaa ttcgattctt
     1321 ccggctcctt gctttggtcg catacgcaca tggccggatt gaacgatcgg gggaagcacg
     1381 cttttcaaac ggcggagggt aactatgcga tcgtgggtac ggctggtgcc ggcagcggtt
     1441 cttccgcatt ttatttcctg gtaatggata cttcgggggc aatcgtcctc aacctggttg
     1501 atgggtttcc gaaacccaat tacctgcatt tttatccgaa cccggcaaag gggatcgtcc
     1561 gtctcgctcc tggtcccgtc aaagacctgg taatcttcga tctcctcggg cgtgtccgga
     1621 tgcgcgttcc ggaagcacgg gaaagtttgg atctgggatc attattaccc ggttgttata
     1681 tcgtggaagc ggagaacgag tcgggggcgc accttcaggg taaactaatc gttgcggaat
     1741 gagtccggaa acttaccagg tggtcggatt aatgtccggt acttcgctcg acggactgga
     1801 tatcgcgttg tgtgagttcg tccgaggcga tacctggagc tatcggatcg tggaagcgga
     1861 gaccgtgacg tatcccgaaa gcatcgcatc cctgttgcga agcattgata aagcggatgc
     1921 tgaatcgctt gtaaaagcgc aggtacatta tgggagttac ctcggggaac aagtccggaa
     1981 gttcctggcc gaacgcagct gcagcgcgga tttcgtctcc tcccacgggc acaccatcta
     2041 ccatcaaccc ggtaacggtt ttacgttcca gttgggcgat ggcgccgcac tttcggtagc
     2101 tgcgggattg ccggtggtga gtgatttccg catgcaggat gtggcgctcg gaggacaagg
     2161 cgctccgctc gtgccggtgg gcgaccaact gttgttcggt gaatttacgc actgcctcaa
     2221 cctgggtggg atcgcgaaca tatcgttctc agcgggtaat cgtcgtgtag ccggagacct
     2281 gtgcccgtgc aaccttttgc tcaatccggt agctgcacgg cgaggactac ccttcgaccg
     2341 cgatggccag ttggcccaaa acgg
//
LOCUS       DAOTON010000449         2360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_35218_length_2360_cov_3.066811, whole genome shotgun sequence.
ACCESSION   DAOTON010000449 DAOTON010000000
VERSION     DAOTON010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35218_length_2360_cov_3.066811"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="P5292_08965"
     CDS             <1..588
                     /locus_tag="P5292_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39295.1"
                     /translation="VNNLAPGTYTVQVSDYNGCPDFATVTIGYLFPAPVVDLGPDTTI
                     CNGATLTLDAGAGYQYLWSDNSTNQTLDVTAAGVYSVLVTDANNCEAFDAITVSTVVC
                     IRYNHSTTTLARRSFGIYPNPSTGVVDINFSNESAADVQIDVVDVYGKALFHASENLK
                     AHDLRKYDLSNLSPGIYFVRVSYGSETQSIRLIKQ"
     gene            1511..2023
                     /locus_tag="P5292_08970"
     CDS             1511..2023
                     /locus_tag="P5292_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39296.1"
                     /translation="MRRLLLAATLILLAFAGRAQKLDSEVLFKQYFSKNADSLDAIEG
                     IWNVSTTQEFYRYDTLYDIQTFAKAARVAIMKKEGKYDSYNLTGESYDVEFQTTDVKG
                     VYLYRNFFRETNEYSKTQAVISKAGEMEYTYDFPDNYLRVKFQDSYEEGTRVVNILKW
                     TKVFPDGKKK"
     gene            2020..>2360
                     /locus_tag="P5292_08975"
     CDS             2020..>2360
                     /locus_tag="P5292_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39297.1"
                     /translation="MSRTRRYLLPLFFVFLLSSAQAAEKWWVTFRDKGPADRQLISDS
                     TDWPVDPTYLSAVAAEVRTITGKSNWLNGAAVVATGAQLDRLRALDCIASLEPMAPSE
                     RSQKQKREHAYR"
BASE COUNT          434 a          730 c          635 g          561 t
ORIGIN      
        1 gtgaacaacc tggctcccgg cacatacacg gtccaggtat ccgactacaa cggctgtccg
       61 gatttcgcga cggtgacgat cggttacctg ttcccggcac cggtggtcga cctgggtccg
      121 gataccacca tctgcaacgg cgccacgctc acgctggatg ccggtgcggg ttatcagtac
      181 ctgtggtcgg acaactccac caaccagacg ctggatgtta cagcagccgg tgtgtactcg
      241 gtgctggtga ccgacgcgaa caactgcgaa gcgttcgatg cgatcaccgt gagcacggtg
      301 gtgtgcatcc gctacaacca cagcacgacg accctggcgc gacgcagctt cggtatctac
      361 ccgaacccga gcacgggcgt ggtggacatc aacttcagca acgagtccgc ggcggatgtg
      421 cagatcgatg tggtggatgt gtacggaaag gcgctcttcc acgcgtcgga aaatctgaaa
      481 gcgcatgacc tgcggaagta cgacctcagc aatctgtcgc cgggcattta tttcgtccgc
      541 gtatcctacg gatccgaaac ccaatcgatc cgcctgatca agcagtaagg cagtagcgat
      601 atagttcaaa agggcgaagg tttttcttcg cccttttttg tttcatatga ttttcggagc
      661 agtaggcggt aggcggttgg cagtaggcgg tacgcggttt gcggtattca gtatgcagct
      721 tgattccgac acacccttcc tctcaccaca tttcctcgtc cctcgtccct cgccccacgt
      781 ccctccttca tagtcgactg ttgttacgcc cctccggggc tgggtggaag ttgggtgttc
      841 gttgtctgac atgttgggcc cctccggggc tgggcgattg gtgaattgta ccgaattgat
      901 tggtgtgttc agtttgcagt aggtagatta cacgctcctc gcacctcgca tctttttccc
      961 tcggtctcgg tctcgttcct cgttcctcgt tcctcccttc cccgtccctg cctacgctaa
     1021 agcttcggca ggcaagcctc gcatcacgca tctacttttc ctcgcccctc gtccctcgtc
     1081 ccacgtccct ccttcatagt cgactgttgt tacgcccctc cggggctggg tggaagttgg
     1141 gtgttcgttg tctgacatgt tgggcccctc cggggctggg cggttggcag taggcggttt
     1201 gcggtattct gtatgcagct tgattcctac acttcctttc acctcgcacc tcgcacccct
     1261 cctctcaccg catttcctcg tccctcgtcc ctccttccct cggtcacggt ctcgttcctc
     1321 gcatcttcct tcctggtcga ctgttgttac gcccctccgg ggctgggcgg ttggcagtag
     1381 gcggtttgca gattgcagta tgcagtatgc agtaaaatat cgaaacgccc attcctcgca
     1441 cctcgcatca cgcatctcat ttcctcgtcc ctcgtccccc gcccctcgtc cctttttcct
     1501 atctttcgcc atgcgccgcc tcctgcttgc cgctacgctg atcctgcttg ctttcgccgg
     1561 tcgtgctcag aagctcgaca gcgaagtgct tttcaaacag tatttttcca agaacgccga
     1621 cagcctcgat gccatcgagg ggatttggaa cgtgtcgacg acccaggagt tttatcgtta
     1681 cgatacgctt tacgatatcc agaccttcgc caaggctgcg cgggtggcaa tcatgaagaa
     1741 ggaggggaag tatgattcct acaacctcac gggtgagtcg tacgatgtgg aattccagac
     1801 gaccgacgtg aagggcgtgt acctgtaccg gaatttcttc cgcgagacca acgaatattc
     1861 caagacccag gccgttatca gcaaagccgg ggaaatggag tacacctacg atttcccgga
     1921 caactacctc cgcgtgaagt tccaggacag ctacgaggag ggcacccgcg tggtcaacat
     1981 cctcaaatgg accaaggtct ttcccgacgg caaaaagaaa tgagtcgaac gcggcggtac
     2041 ctgttgccgc tctttttcgt tttcctgctc tcctccgcac aggctgcgga gaagtggtgg
     2101 gttacctttc gcgataaagg tcccgccgat cgccaactga tttccgattc caccgactgg
     2161 cccgtcgacc cgacgtattt gtcggccgta gccgcggagg tgcgcacgat caccggtaaa
     2221 agcaactggc tcaacggcgc ggcggtggtc gcgaccggtg cgcaactgga ccggctgcgc
     2281 gcgctcgact gcattgcctc actcgagccg atggcgccca gtgaacgttc gcaaaagcaa
     2341 aagcgggaac atgcgtaccg
//
LOCUS       DAOTON010000450         2358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_35264_length_2358_cov_2.866261, whole genome shotgun sequence.
ACCESSION   DAOTON010000450 DAOTON010000000
VERSION     DAOTON010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35264_length_2358_cov_2.866261"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="P5292_08980"
     CDS             complement(<1..176)
                     /locus_tag="P5292_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRS39298.1"
                     /translation="MFRDLQPDLSAASWAVRFAASQKGVLMVLSGMSNIEQLRENISY
                     MIDIVPLNEAELSVV"
     gene            complement(287..1375)
                     /locus_tag="P5292_08985"
     CDS             complement(287..1375)
                     /locus_tag="P5292_08985"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HRS39299.1"
                     /translation="MTINAGVDLHKTQFTVFVKQSGNQFEQYSTEEDGYKKFLTRIQR
                     WKKSGMTVRIGVESTGNTRYFKTRMEQAGAEVVVINTLKFKVVNESVKKTDRHDAATI
                     AEFLEKDMLPESRLCSEESEQLRRLLKVRTTLVRTMVTVKNQIHALLTGLGMKDSRAT
                     LQSKRGRQKILDALRKADSELVVQPLFETIEQLEKSVKSIERELEKLTEGDRTVELLM
                     TIPGCGKIGAWTIRAFTDDINRFASAKKYSAYAGLVPWVQNSNETVHHGKITKRGPEE
                     LRTALVQLVMGMRRCRSTVSWRLMERYTEMKRNKGSGKSIIATARKLSTIIWCMLSTG
                     QEFDPSLMIDRQLIRKADSMRTTASQSA"
     gene            complement(1585..1767)
                     /locus_tag="P5292_08990"
     CDS             complement(1585..1767)
                     /locus_tag="P5292_08990"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRS39300.1"
                     /translation="MKEDWVEASLPDVCEIQTGKYDANHAKQNGKYKFFTCAFESLKC
                     DTNRFKGGMIKRVNRE"
     gene            complement(1767..>2358)
                     /locus_tag="P5292_08995"
     CDS             complement(1767..>2358)
                     /locus_tag="P5292_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HRS39301.1"
                     /translation="RVTKSEWDSFTTEKAKETIRDFSEYLARHKDEIQALSIFYDQPY
                     NRRSITFTMIKEVMDKIRLEKPLLAPDYVWNAYASVENVRSGQPRDELTALVSLIRRV
                     CGIDKELKPFDKTINENFQNWIFRQNAGQHNRFTAEQMEWLRMIKDHVVSSYHIAIDD
                     LDYTPFDARGGRGRMHQLFGNEMNKIIDELNEVLAA"
BASE COUNT          526 a          660 c          467 g          705 t
ORIGIN      
        1 acaacagaca gttctgcttc attgagcggt acaatatcga tcatgtacga aatattttcg
       61 cgaagctgtt caatattgga catgccgctc aaaaccatga gcacaccctt ctggctcgct
      121 gcgaaacgga cagcccagga cgcggcggat aaatcgggct gcaagtcccg gaacatcttg
      181 tccgcattct ccggaaccga cgcaatggta ccacccttga ccggttccta taaaaagtca
      241 accgggaggt tgaccaattc catacttcaa tccgacaacg tcggttttat gcactctgac
      301 ttgcagtagt tcgcatcgaa tcagcctttc gaattaattg cctgtcgatc atcaaggatg
      361 gatcgaattc ctgaccagtg cttaacatgc accaaatgat cgtcgacaac tttctggctg
      421 tcgctattat cgatttcccg cttcccttgt tcctcttcat ctctgtatat cgttccataa
      481 gtcgccatga taccgttgat cgacaacgtc tcattcccat taccaattga accaaagccg
      541 tccgtaactc ttccggaccg cgtttcgtta tttttccatg atgtactgtc tcgttcgagt
      601 tctgtaccca tggaacaagt cctgcatacg cagaatactt ctttgccgat gcgaatcggt
      661 tgatatcatc cgtaaaggcc cgaattgtcc aggcaccgat ttttccgcat ccgggtatcg
      721 tcatcagcaa ctcaaccgtg cggtcgcctt cagtcagctt ctcaagttct ctttcaatac
      781 tcttgacgct tttttcaagc tgttctatcg tttcaaaaag cggttgaact acgagttcgc
      841 tatccgcttt tctgagggcg tccagaattt tctggcgccc tctcttgctt tgcagggtcg
      901 ccctggaatc cttcatccca agtccggtca gtaacgcgtg tatctgattt ttcaccgtta
      961 ccatggttcg caccaacgtc gttctgactt tcagcaatcg tctaagttgt tcgctttctt
     1021 ccgaacacaa ccgtgattcc ggcaacatat ccttttccag aaactcggct atcgtcgctg
     1081 cgtcatggcg atcagttttc ttgaccgatt cattgaccac cttgaacttc agcgtgttga
     1141 tcactacaac ctccgcgcct gcctgttcca tccgtgtttt gaagtaccgc gtattcccgg
     1201 tcgactcaac tccgatacgt accgtcatcc cgctcttttt ccagcgctgt attcgggtaa
     1261 gaaacttctt gtatccgtcc tcttcggtcg aatactgctc aaactgattc ccgctttgtt
     1321 tcacaaacac agtaaactgc gtcttgtgaa gatctacacc tgcattgatt gtcataccgc
     1381 tactcccttt tccgacaaag gtctattcgg tacattggat ccattcctct cgaagcttcg
     1441 ctgagaatcc tattcggcct ctcgggccat ggaatgatgc ggccaaagct cttcattctc
     1501 cttttcggtc tcaacggacc agtaaacatg acgaactcca gccttcctct gtcggcacac
     1561 ttccaacagg ataatccaat ttacctactc ccggttgact ctttttatca ttcctccttt
     1621 aaatctattt gtatcacatt ttaaagattc aaaagcgcaa gtaaaaaact tatattttcc
     1681 attttgtttt gcgtgattag catcatattt acctgtctgt atttcacaaa catctggtaa
     1741 acttgcttcc acccaatctt ctttcatcac gctgctaata cctcgttcag ttcatcaatg
     1801 attttgttca tctcgttgcc gaaaagttgg tgcattcggc ctcggccgcc gcgggcgtcg
     1861 aagggggtgt aatccagatc gtcaatggca atatgataac tgctgaccac gtgatccttg
     1921 atcatccgca gccattccat ctgctccgcg gtaaagcggt tgtgctgccc ggcgttctgc
     1981 ctgaaaatcc agttctggaa attctcattt atcgttttat caaaaggttt caattccttg
     2041 tcgatgccgc agacccggcg gatcagggaa accagtgccg ttaactcgtc cctgggctga
     2101 ccgctcctaa cattctctac agacgcgtag gcgttccaca cataatccgg agccagcaat
     2161 ggtttttcca gcctgatctt gtccatcacc tccttgatca tggtaaaggt aatgcttcgg
     2221 cggttgtacg gctgatcata gaaaatgctt aacgcctgaa tctcgtcctt atgccgggcc
     2281 aggtactcgg aaaaatccct gatggtttct tttgccttct cggtggtaaa actgtcccat
     2341 tcagatttgg taacccgg
//
LOCUS       DAOTON010000451         2357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_35277_length_2357_cov_3.466551, whole genome shotgun sequence.
ACCESSION   DAOTON010000451 DAOTON010000000
VERSION     DAOTON010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35277_length_2357_cov_3.466551"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="P5292_09000"
     CDS             complement(<1..1054)
                     /locus_tag="P5292_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39302.1"
                     /translation="MEITIGLFSDCGATGQQVYTSVDVNFSSNSCGWLGVQTLPLING
                     GGVEVSTGPYPVCGVSSCQGGTAYGIRKYVYQGVLQLQGACTDWLVSCELLNRNTVVT
                     SLVSPQDYSLHLEAGFDNQNFPNDSSTVFANPAVLLSCVNDGLFYDPLYTDAAADSVR
                     ALLIPSQGIPGPGQPLTNVPYAIGYSPSLPFQTTAGFQFDPVSGIFSGQPAIQEGATV
                     ACRFERYRNGSLAGYVVREVQVLIALTCNRPPALSTATIDTMNCGDTNLVLHFAMPLR
                     CNSVATDGSDFRLLGPDGQPLPITGATAMNCSNGLFTDLELQLFQPLITNGILRLWCK
                     TGSDANTLLNSCGDAQG"
     gene            1782..>2357
                     /locus_tag="P5292_09005"
     CDS             1782..>2357
                     /locus_tag="P5292_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRS39303.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLEFTQTISKKDLQAQVLDDM
                     DLERERGITIKSHAIQMQFAFKGEKYVLNLIDTPGHVDFSYEVSRAIAACEGALLIVD
                     AAQGIQAQTISNLYLALEHELEIIPVLNKIDLPSANPEVVKDQIVDLVGCNREDIIPA
                     SAKTGLGIEDILAAIVERIPSPKGEPAAPLKA"
BASE COUNT          599 a          630 c          611 g          517 t
ORIGIN      
        1 cgccctgcgc atcgccgcaa ctgtttaaca gggtgttcgc gtcgctgccg gtcttgcacc
       61 acagccggag aattccgttg gtgatcaagg gctggaagag ttgcaattcc agatcggtga
      121 aaagtccgtt ggaacaattc atcgcagtcg caccggtgat cggcagcggt tgcccgtccg
      181 gtcccagcag gcggaagtcg gagccgtcgg tggcgaccga gttgcagcgg agcggcatcg
      241 cgaagtgcag gaccaggttg gtgtctccgc aattcatcgt gtcgatcgtg gcggtcgaca
      301 aggccggtgg tcgattgcac gtcaatgcga tcagtacctg tacttcccgg accacatagc
      361 cggcgaggct gccgttgcgg tagcgttcaa atctgcacgc gaccgtcgcg ccttcctgga
      421 tcgccggctg accggagaag atgccgctga ccggatcaaa ttggaatccg gcagttgtct
      481 gaaacggaag agaaggggag tagccgatcg catacggtac attcgtcagc ggttgccccg
      541 gccccggtat tccctgcgac ggaatcaaca gggctcgaac actatcggct gctgcgtcgg
      601 tataaagtgg atcgtagaaa agcccgtcgt tcacacagct aagcagtacg gcagggttgg
      661 caaacaccgt ggacgaatcg ttcggaaaat tctggttgtc aaaaccggct tccaggtgca
      721 gcgaatagtc ttgcggactc accagcgagg tcaccacggt gttccggttg agcaattcgc
      781 aactcaccag ccaatcggtg caggcaccct gcaattgcaa cacgccctgg tacacatact
      841 tccgaatgcc ataggcggta ccaccctggc aactgcttac accacaaaca gggtaaggtc
      901 cggtcgatac ttccacacca ccgccgttga tcaatggtaa ggtttggacg cccagccaac
      961 cgcaggaatt cgaactgaaa ttgacatcca cactcgtgta aacctgctga ccggttgctc
     1021 cgcaatccga gaagagtcca atggtaatct ccacccggtt agcccccagg caacggtacc
     1081 caatttcgcc accagccacg tgtgtagcgg tagcgcgaaa cgcgggtagg ctgataagaa
     1141 gccaaaaaat cgttaaaaag atggttttca gggcctgacg cttaagctaa tcctttacga
     1201 gaaccagcag gcggcggtta ccaggttatt ggagaagtga gattagtgat ttgagattcg
     1261 atattggtga tttgtcccag gaatgtgcga attgatagtt gaatgcagat cgaaaggatc
     1321 tgacgtttaa aataattgaa aatcaataaa ttgaataaga aatggcagtc actcaatatt
     1381 gcgtaagttg cttatttaca gatagttgat tacttccggc ggtgttacgc taattctgga
     1441 aggtgttacg gtatcctgaa aagagactcg aagaatgata aaggctttat tttagtaaaa
     1501 aatggagctt gtaaactcca aagctcaatt aatgctcgca ttcaaatcct ttcaggaaag
     1561 ccaccaatat tttcaccgcc gttttaatta ccaattacta attactaatt acgaatcacc
     1621 aatcaccaat cacccattcc aaaacaccaa cccccatccc tgcctcccct aatttgccta
     1681 cttttgcaac ccgcccggga ttcgggaggc tgaaatttcg aaattgttcg aaattgctga
     1741 taatgaacaa aatggacctc gggtccggaa tctgaccgcg cgtgaagaac atccgcaatt
     1801 tttgcatcat cgcccatatc gaccacggta agagcaccct ggcggatcga ctcttggagt
     1861 tcacccagac catctccaaa aaggatttgc aggcgcaggt tctcgacgac atggacctgg
     1921 aacgtgagcg cggtatcacg atcaagagtc acgcgatcca gatgcagttc gccttcaagg
     1981 gcgagaagta tgtgcttaac ctgatcgata cgcccggaca cgtcgacttc tcctacgaag
     2041 tatcacgcgc catcgcagct tgcgaaggag ctttgctgat tgtcgatgcg gcgcagggca
     2101 tccaggcaca aaccatttcg aatctctacc tcgcgctcga gcacgaactc gagatcattc
     2161 cggttctcaa caagatcgac ctgccttccg ccaacccgga agtggtgaag gaccagatcg
     2221 tcgacctggt cggttgtaat cgtgaagaca tcattccggc ttccgccaag acaggactgg
     2281 gcatcgaaga cattctcgct gccatcgtgg agcgtatccc ttcgcccaaa ggcgagccgg
     2341 ctgctccgtt gaaggct
//
LOCUS       DAOTON010000452         2332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_35781_length_2332_cov_3.436539, whole genome shotgun sequence.
ACCESSION   DAOTON010000452 DAOTON010000000
VERSION     DAOTON010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35781_length_2332_cov_3.436539"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /gene="gcvT"
                     /locus_tag="P5292_09010"
     CDS             <1..814
                     /gene="gcvT"
                     /locus_tag="P5292_09010"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRS39304.1"
                     /translation="GGIVDDLLVYRIDEQTYMLVVNASNIEKDWNWIRKQDEWNVEMH
                     NISDKTSLLAVQGPLAADVLQPLTNMDLRNMEYYSFAKGVFAGIENVLVSATGYTGAG
                     GFEVYFENGSAGKIWDAIFEAGKTQGIRPIGLGARDTLRLEMGFCLYGNDIDDTTSPI
                     EAGLGWITKFTKPFINSDALKRQKEEGVSRKLVGFEMIDRGIPRHDYEVRDAAGIAIG
                     KVTSGTQSPTLNKAIGLAYVPPAFAREGSEIFIQVRDKALRAKVVKIPFIKK"
     gene            907..1656
                     /locus_tag="P5292_09015"
     CDS             907..1656
                     /locus_tag="P5292_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HRS39305.1"
                     /translation="MSDQPLLKVDACFSPFLYPVYADDTSIVVIIDIFRATSAMCTAF
                     EYGAEKIIPVASVDEARAYKAKGYLTGAERDAIQIEGFDFGNSPYHYMDERIKGQTLV
                     LTTTNGTQAIEAARNAYRVVIGSFLNIDALCEWLVAEKRSVLLLCSGWKNKFNLEDAI
                     FAGAVTEKLSAMSDQFKLGDGCLALKYLYQLAHQDPYKFLNYSSHKERLARLNLKKDI
                     KYCLTPNQTRVIPVMKDGALVRMEVLAGSLD"
     gene            1753..>2332
                     /locus_tag="P5292_09020"
     CDS             1753..>2332
                     /locus_tag="P5292_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRS39306.1"
                     /translation="MKRFVLVLIACFLWAQELDAWGFWAHRRINEMACYLLPEAMFGF
                     YKANIGYITEHATDPDLRRYRDPAEAPRHFIDLDRYGEHPIDSLPHRRKDAAAKYSED
                     TLMAHGTVPYTIAEFYHKLIYAFRDRDRGRILHYSAMLGHYIGDAHVPLHCTRNYNGQ
                     LTGQHGIHGFWESRLPEMFGNGYDYLLGPVGAL"
BASE COUNT          546 a          576 c          646 g          564 t
ORIGIN      
        1 gggtggaatt gtcgacgact tgctcgtgta tcggatcgat gagcagacgt atatgctcgt
       61 cgtaaatgct tcgaacatcg aaaaggattg gaactggatc cggaagcagg acgaatggaa
      121 tgtggagatg cacaacatct ccgacaagac atcgctcctt gctgtacagg gtccgctggc
      181 tgcggatgtg ttgcagccat tgaccaacat ggacctccgg aacatggagt actattcctt
      241 tgcgaagggt gtatttgccg gtattgagaa cgtactggtc agcgcgacag gatataccgg
      301 agccggtggt ttcgaagtgt atttcgagaa tggatccgcc gggaagatct gggatgctat
      361 ttttgaggca gggaaaacac agggcattcg cccgatcgga ttgggtgctc gcgacacctt
      421 gcggctggag atgggtttct gtctttacgg caatgacatt gatgatacca cttcccccat
      481 cgaagcagga ctcggctgga tcacgaagtt taccaagcca ttcatcaaca gcgatgcact
      541 caagcggcag aaggaagaag gggtaagcag aaagttggtc ggtttcgaaa tgatcgatcg
      601 cggtattccg cgacacgatt atgaggtgcg ggatgcagcg ggcattgcga ttggtaaagt
      661 gacgagtggc acccaatctc caaccttgaa caaggccatc gggttggcgt acgtcccgcc
      721 agcgtttgcc agggaaggtt cggagatatt catccaggta cgcgacaaag cgctgcgcgc
      781 gaaagtggtc aagattccgt tcatcaagaa ataagggaaa ttctactgtt tttaccgtcc
      841 cggtaaccgg acggcgatca ttccggaatt cctttatctt cgttttttaa accagattag
      901 ttgctgatga gtgatcaacc gctcctgaaa gtcgacgcct gcttttctcc ctttctttat
      961 ccggtctatg cggatgatac cagcatcgtg gtgatcattg atattttccg ggccacctca
     1021 gccatgtgta cggctttcga atatggtgcg gaaaagatca ttcccgtggc ttctgtcgat
     1081 gaagcgaggg catataaggc gaagggttat ctcaccggtg cggaacggga cgcgatccag
     1141 atcgaaggat tcgactttgg aaattcccct taccattata tggatgagcg gatcaaaggt
     1201 caaaccctgg tcctgactac caccaacgga acgcaggcga tcgaagcggc tcgtaacgct
     1261 tatcgcgtgg tgatcggctc attcctcaat atcgacgccc tttgcgaatg gctggtggcc
     1321 gagaagcgga gtgtgctctt gttgtgttcc ggttggaaga acaagttcaa tctggaggat
     1381 gcgattttcg ccggagcggt aacggaaaaa ctctcagcca tgtccgacca gttcaaactg
     1441 ggtgacggtt gccttgcgtt gaagtatctc tatcagttgg cccatcagga tccctataag
     1501 ttcctgaact actccagcca caaggaacga ctcgcgcgtc tcaacctgaa gaaggatatc
     1561 aagtattgcc tgacacccaa ccagacacgg gtgattccgg tgatgaaaga tggcgcactg
     1621 gttcgcatgg aggtgctggc tggatctttg gattagactt aatcggtatc gaccgttttc
     1681 atctcagcaa attcatatta gttcccgcat ttattggacg acggatagtg gtccgagcgg
     1741 tattcttgta cgatgaaacg attcgtactc gtattgatcg cctgctttct ctgggcacag
     1801 gaactggatg catgggggtt ctgggcgcac cggcgcatca acgaaatggc ctgttatctc
     1861 ttgcccgaag ccatgttcgg attttacaag gctaacatcg gttacatcac cgaacacgcg
     1921 accgatccgg acctgcgccg ctatcgtgac cccgcggaag ctcccaggca tttcatcgac
     1981 ctcgaccggt atggggaaca tccgatcgac agcttgccgc atcgaagaaa ggatgctgct
     2041 gccaaatact cggaagacac gctgatggcc catggtacgg tgccgtacac cattgcggag
     2101 ttttatcaca agctgatcta tgcgttccgg gaccgtgatc gcggacgtat cctacattac
     2161 tccgccatgc tgggtcatta tatcggtgac gcgcacgtcc ccttgcattg cacacgcaac
     2221 tacaatggcc agctcaccgg tcagcacggc attcatgggt tctgggaatc ccgcttgccg
     2281 gaaatgtttg gaaacggtta tgactacctg ctcggaccgg ttggcgcgtt at
//
LOCUS       DAOTON010000453         2328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_35863_length_2328_cov_3.359437, whole genome shotgun sequence.
ACCESSION   DAOTON010000453 DAOTON010000000
VERSION     DAOTON010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35863_length_2328_cov_3.359437"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2328)
                     /locus_tag="P5292_09025"
     CDS             complement(<1..>2328)
                     /locus_tag="P5292_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39307.1"
                     /translation="IPRILIPATNLVCNSKGDSLMIRETSGAYYPTKQEFFGNGGKVD
                     WTRAGWDAGVVRAELARFRIDVTGSDFEADSVTFYNSTYFQAPLTGKYTDKLLANMGP
                     ENASYPRFVSYTTSLEIKNIVPDVNYRGGFSMIGNRMVGSGSVEEQAKLIFSREKKPF
                     LIAGSDGFVVRKDRITADQASVLFNLDGDSIYHPSITFKYMVADREVTLIRAEEGKSQ
                     APYYDSFHQIDMYYDGLYWKIDDPLIDLKMISGAGESKATFESSNYFRKNRFLKLQGL
                     SDVHPLFTIKQFAQSQGNNIVYTEDLAKYMRLAPSEVRTMLINFSNGGFVSYDSKEDR
                     AIIKARLYYYLQANVGKTDYDVLQFESVIKARPNASINLLNYEMTLRGLAPIVLSDSQ
                     NVVIFPKEQEVKLKKNRDFTFAGRVKAGRFDFFGKQFSFDYHNFKINLDNVDSLRLRV
                     ESDDPSEVDEFGNRKLVAVRSVVENITGDLLIDQFGNKSGLKDFPQYPIFNSKKDSYV
                     YYDRPFIQEGVYTRDDFYFHLDPFTIDSLDNFSRAGLGFAGEFVSAGIFPDFRDSLKL
                     QPDLSLGLVRQTGDAGWQAYGGKGKVTAKLSLSNAGLHGDGTLDYLTSVSKTDDFLFL
                     PDSMNSNVKSFTNRNETLGGVQYPSVIGDEVYVHWEPKKDVMYVNRRQEDLKMYEQQA
                     TLNGNLQLRPAGMTGNGLMAFTGSELESKLFRYKSEQFDADTSDFRLTSDNASALAFD
                     TKNVNAHIDFVKRMGEFKSNGAGSYVNFPLNQYIC"
BASE COUNT          540 a          617 c          607 g          564 t
ORIGIN      
        1 aacagatgta ctggttcaac gggaaattga catacgaacc ggcaccattc gatttgaact
       61 cacccatccg cttgacaaag tcgatgtgcg cgtttacgtt cttggtatcg aaggcaagcg
      121 cggaggcatt gtcggatgtg agtcgaaaat cggaggtgtc agcgtcaaac tgctctgatt
      181 tatagcgaaa cagttttgac tcgagttcgc ttccggtgaa tgccatcagc ccgttcccgg
      241 tcataccggc aggtcgcagt tgcaggttgc cgttcagcgt agcctgctgt tcgtacatct
      301 tcaggtcctc ctgccgacgg ttgacataca tgacgtcctt cttcggctcc cagtgcacat
      361 acacttcatc gccaatcaca gaggggtact ggactcctcc cagcgtttca ttccggttgg
      421 tgaagctctt cacgttggag ttcatcgaat ccggcaggaa caggaagtcg tcggttttcg
      481 aaacagaggt caggtaatca agggtaccgt caccgtgcag gccggcattg ctgagggaga
      541 gcttggcggt gactttacct ttacccccat atgcctgcca tccggcatcg cccgtttgcc
      601 ggaccaggcc cagggacaga tcgggctgga gtttcaacga atcccggaag tccggaaaga
      661 ttccggcgct gacaaactca cccgcgaacc cgaggcctgc acgactgaag ttatcaagcg
      721 aatcgatcgt gaacgggtcc aggtgaaaat agaaatcatc ccgggtgtaa acgccttcct
      781 ggataaacgg acgatcataa tagacgtagg agtccttctt ggagttgaaa atcgggtact
      841 gcggaaagtc ctttaatccg gacttattcc cgaactggtc gatcagcagg tcaccggtga
      901 tgttctcgac cacgctcctg acggctacca gcttgcgatt gccgaattca tccacttccg
      961 aaggatcatc gctttccacg cgcaaccgaa gcgaatcaac gttgtcgaga ttgatcttga
     1021 aattgtggta atcgaacgaa aactgctttc caaaaaaatc gaagcggccc gccttcaccc
     1081 ggccggcaaa ggtgaaatcc cggttcttct tgagcttcac ttcctgttct ttcgggaaaa
     1141 tgaccacgtt ctgcgaatca ctcaatacga tcggtgccaa cccccgcagc gtcatttcat
     1201 aattcaggag gttgatgctg gcattcgggc gcgccttgat gaccgattcg aactggagta
     1261 catcgtagtc cgtctttcct acattcgcct gcaggtaata atagagccgg gctttgatga
     1321 tggcgcgatc ttctttgctg tcgtagctga cgaaaccacc attggagaaa ttgatcaaca
     1381 tggtccgcac ctcgctggga gcaaggcgca tgtacttggc gaggtcttcg gtatagacaa
     1441 tgttattacc ctgcgactgc gcgaattgct tgatggtaaa caaggggtgg acatcgctca
     1501 gcccctggag tttgagaaaa cggttcttcc gaaagtaatt cgaggattcg aatgtggctt
     1561 tgctttcccc cgcaccgctg atcattttca gatcgatcag cggatcgtcg atcttccagt
     1621 acaatccgtc gtagtacatg tcgatctgat ggaacgaatc ataatacggt gcctgtgact
     1681 tcccttcttc cgcacggatg agcgtcactt cacgatcggc taccatgtac ttgaaggtga
     1741 tgctggggtg atatatgctg tcgccgtcga ggttgaacag cacggaggcc tggtcggcgg
     1801 tgatccgatc cttacgaacg acgaagccgt cggaaccggc gatgaggaag ggtttctttt
     1861 ctcgggaaaa gatcagcttg gcttgttctt caacggaacc tgaacctacc atgcggttgc
     1921 cgatcatgga gaaaccgccc cggtaattca catcgggtac gatattcttg atttcaagac
     1981 tggttgtata agaaacaaaa cgcggataag aggcattctc cggtcccatg ttggccagca
     2041 gcttatccgt gtacttcccg gtcagtggtg cctgaaaata ggtcgaattg tagaacgtca
     2101 cgctgtcggc ttcgaagtcg ctccccgtta catcgatccg gaaccttgcc agctcggcac
     2161 gcacaacacc tgcatcccag ccggcccgcg tccagtcaac ctttccaccg tttccgaaaa
     2221 actcctgctt ggtcggataa taagcaccac tggtttcccg aatcatcaac gaatcacctt
     2281 tggagttaca gaccaggttg gtggccggga tcaggatacg tggtatgg
//
LOCUS       DAOTON010000454         2323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_35962_length_2323_cov_2.686949, whole genome shotgun sequence.
ACCESSION   DAOTON010000454 DAOTON010000000
VERSION     DAOTON010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35962_length_2323_cov_2.686949"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="P5292_09030"
     CDS             complement(<1..512)
                     /locus_tag="P5292_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39308.1"
                     /translation="MSRLGQQVERVERVVRSWKPGESVARHLSDHFSCHRNMGARDRR
                     ECRAWVYAWIRLGRSLEDLPFAKRLAVATYLTTPEPYPALEALLNSELSDIELPSLQS
                     SVEEKTNAVARSFPGFSLEKIFPGSSRISSGLDRAAFLSGLFLPPELWIRVRTKYREQ
                     VIQELTDRSIP"
     gene            582..>1167
                     /locus_tag="P5292_09035"
     CDS             582..>1167
                     /locus_tag="P5292_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39309.1"
                     /translation="MLRSLTALLIALWCFTACTSSDHRATNSVPPPVQDSVPAPAPPV
                     RDTLTTLIPGSYLESVTNSGQAYILYIPRSFRDSLPMPLMVCFDPQGNGRVPVHRYAS
                     LADEFGFILAGSNQVRNGLGIDASASMARELAAAVLEKMKTTHAGLFLCGFFGGAKVA
                     LVAPGNMKGVSGVVHCGAGLPDGIPAPTVPTYGVT"
     assembly_gap    1168..1177
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1178..1996
                     /locus_tag="P5292_09040"
     CDS             <1178..1996
                     /locus_tag="P5292_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39310.1"
                     /translation="NRDMNYSEVIEFVNRLGRQPMHHLDITDGKHAWPETTDMRRAFS
                     WCKVLACKSGGNCERPVLQQFTRQINTLAARTTDPIQRMRIYRCGIDCLQGLSPVPEW
                     ENSVQRLMKDPVYQQSILEDSRALSAELNRRQFLLDAFRSQEEDWWSHVIGDLQKRKQ
                     DPMNDRLLGYISLACYSLTRQAMEQGNMPEVARLNRIYLLADQENAEPNFLAAKLFSQ
                     VGQKDSVLSYLKRSVQYGFKDLSRLESETAMRALLSSADQRDLEEGIRKNQSDW"
     gene            complement(1981..>2323)
                     /locus_tag="P5292_09045"
     CDS             complement(1981..>2323)
                     /locus_tag="P5292_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRS39311.1"
                     /translation="GTIRLIDEIHTPDSSRYFYADGYAERQAKGEQQKQLSKEFVRQW
                     LIANGFQGRAGESVPEMPDTWLDEISSRYIELYEKLTGESFTPAPPGDPRERIEYNVR
                     RALSLLGYQSD"
BASE COUNT          527 a          662 c          642 g          482 t
ORIGIN      
        1 ggtatggatc gatccgtcaa ctcctgtatg acttgttccc gatacttcgt ccggacacga
       61 atccacaatt caggaggaag gaacaagccc gacagaaaag ccgcccggtc aagaccggaa
      121 gaaatgcggg aggaaccggg gaatattttt tccaggctga atcccggaaa ggaccgggca
      181 accgcgttgg tcttctcttc caccgacgac tgcaatgacg ggagctcaat gtccgacaac
      241 tcggaattca acaacgcctc cagtgccgga taaggttccg gagtcgtcag gtaagtggca
      301 acagcaaggc gtttcgcaaa agggagatcc tccagggagc gaccgagtcg aatccaggca
      361 tagacccagg cacgacattc ccggcggtcg cgtgcgccca tgttgcggtg acaggaaaaa
      421 tgatcggaga gatgacgcgc gaccgattcg cccggtttcc aggatcgcac cacccgttcg
      481 actcgctcta cctgttgtcc cagccggctc atgggttgca aagaaacgaa ttcaccgtgt
      541 acccatcttc ctgagcgcgc gaaagagtag tttcgcaaac aatgctacga tcactgaccg
      601 ctctcctcat cgcgctgtgg tgtttcacag cctgtacttc gtcggatcat cgggcgacga
      661 attccgttcc gccgcctgtg caagacagtg tacctgctcc agccccgcct gtccgggata
      721 cgctgacgac actgattcca gggagctatc tggaatcagt caccaacagt gggcaggcgt
      781 acatcctcta cattccccga tcctttcgcg acagcctgcc catgccgttg atggtgtgct
      841 ttgacccgca aggcaacggt cgcgtgccgg tacatcgcta cgcatccctg gccgatgaat
      901 tcggttttat tctggccgga tccaatcaag tacgaaacgg cctgggcatc gacgcatccg
      961 cttccatggc ccgggaactt gcagccgcgg tactcgagaa aatgaaaacc acccacgcgg
     1021 gtctgttcct ctgcgggttt ttcggcggcg ccaaagtcgc gctggtagct cccggtaaca
     1081 tgaaaggtgt ttcgggcgtg gtgcattgcg gtgccggact tccggatggg attccggcgc
     1141 ctacggtacc aacctatggt gtgacggnnn nnnnnnnaat cgggatatga actactccga
     1201 agtaatcgaa ttcgtcaacc gtctgggtag acaacccatg catcacctgg acatcaccga
     1261 tggaaaacat gcatggcccg agacgaccga catgcgccgg gcatttagct ggtgcaaggt
     1321 actcgcctgc aaatccggcg ggaattgcga gcgaccagta ctccagcaat tcaccagaca
     1381 gatcaacacc ctggctgcca ggaccactga cccgatccaa cgcatgcgga tctaccgatg
     1441 tggaatcgat tgtctgcagg gattgtcgcc ggttcccgag tgggaaaata gtgtccagcg
     1501 gttgatgaag gatcctgtct atcagcaatc catactggaa gattcacggg cgctctctgc
     1561 agagcttaac cgccggcagt tcttactgga tgcgtttcgt tcccaggaag aggattggtg
     1621 gtcgcacgtg atcggggatt tgcagaagcg aaaacaggat ccgatgaacg atcggctact
     1681 cggctacatt tcgctggcct gttacagcct gacccgccag gcgatggaac aaggcaacat
     1741 gcccgaagtg gcgcgcctga accggatcta tttactggcc gatcaggaaa atgccgaacc
     1801 gaatttcctg gccgcgaaac tcttctccca ggttggccag aaagactccg tactcagcta
     1861 tctgaaacgc tccgtacaat acgggttcaa ggacctaagc aggctggaaa gcgaaacggc
     1921 gatgcgtgcg ctgctttctt ccgccgatca acgggacttg gaagaaggaa ttcgaaaaaa
     1981 tcagtcggac tggtagccga gcaggctcaa tgcccggcga acattatatt caatgcgttc
     2041 ccgcggatcg ccgggagggg ccggagtgaa cgactcccct gtcagctttt cgtagagttc
     2101 aatgtagcga ctgctgatct cgtccagcca ggtatccggc atttccggca ccgactcccc
     2161 cgctctgccc tggaagccgt tagcgatcaa ccactggcgt acgaattctt tcgagagttg
     2221 tttttgttgt tctccctttg cctgacgctc cgcgtagccg tcggcgtaga aataacgtga
     2281 actgtcggga gtgtggatct catcgatcaa acggatcgtg cca
//
LOCUS       DAOTON010000455         2321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_35994_length_2321_cov_2.712710, whole genome shotgun sequence.
ACCESSION   DAOTON010000455 DAOTON010000000
VERSION     DAOTON010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35994_length_2321_cov_2.712710"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="P5292_09050"
     CDS             complement(<1..207)
                     /locus_tag="P5292_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRS39312.1"
                     /translation="MRIDILTILPELLEGPFSHSILKRAEQKGLAEIHLHNIREFSLD
                     KHRSVDDYAYGGGAGMVMMAEPIVR"
     gene            complement(214..1050)
                     /locus_tag="P5292_09055"
     CDS             complement(214..1050)
                     /locus_tag="P5292_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39313.1"
                     /translation="MRAQDSLQVVGIRSPMSRGEQPGFAIRIPQAKPKELGSGWKKYV
                     RSQSKNSVSLEGNEWIMNRGPVKGLGEDSLISYARFREEAGATLGEFFIGTDDQNFFT
                     GEHAKAALVRSWLREFGVQQYREAVEDELKQAERTLDTYEQELKSLENANENSEKRIR
                     ENKRDIDRLQSEIGENKREQEMKSQQILDQKKTVALYPEGEMRDLEEKKLKQYEKEKR
                     RLEKDKESKEGDIDDKEIENKRMEKSIDENTRNVIPKKQELIGYQKNLVADIRKKLEG
                     IR"
     gene            1216..1683
                     /locus_tag="P5292_09060"
     CDS             1216..1683
                     /locus_tag="P5292_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39314.1"
                     /translation="MKILRIQLLWIGLFFYPFFSVCQAQQTADPTSGILPDWVRMMDD
                     TAVNYNDAVKAFEAFWQGRENPLEEIEEREKYGYSPEREGEIREKLRELSPEEASWRQ
                     EMIWQCKRFEEWQRDVKPFVQNDGRILTPSERKVMEEERNRQLKAAEGKEGKR"
     gene            1680..>2321
                     /locus_tag="P5292_09065"
     CDS             1680..>2321
                     /locus_tag="P5292_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39315.1"
                     /translation="MTRVSCAILFVILFAFTWTSRAQTASWTPLAPALFPTNVSGQIH
                     GISRISQMKFHPSNSSKLYAVSARGGLFISSNGGTSWSLAPGCDAMPNGTRLASVCID
                     HTNDQVLYLGGGDANYYSTGSGVWKSTNGGTTFSQTGLTSGVVVELLMDPTNNNILVA
                     ATSNGIYKTTNGGTNWTLKSGTMAFYDLVKKANASSRVLFATGANTLWRSTDFG"
BASE COUNT          482 a          644 c          599 g          596 t
ORIGIN      
        1 gcgaacgatc ggttccgcca tcatgaccat gcccgccccg ccgccgtacg cgtaatcatc
       61 gacactgcgg tgcttgtcga gtgagaactc ccggatgttg tgcaggtgaa tctccgcaag
      121 ccctttctgt tccgcccgtt tgaggatgga gtgggagaag gggccttcca gcaattcggg
      181 aaggatcgtc aggatatcaa tgcgcatggg gaatcagcga atgccctcga gttttttcct
      241 gatatcggcc accaggtttt tctgataacc gatcaattcc tgctttttag gaatgacgtt
      301 tcgggtattc tcgtcgatcg acttctccat acgtttgttc tcgatctcct tgtcgtcgat
      361 gtcgccttct ttcgattctt tgtctttttc cagcctgcgc ttttcctttt cgtactgctt
      421 cagcttcttt tcctcaaggt cgcgcatctc tccttccgga tacagcgcga ctgttttttt
      481 ctgatcgagg atttgctggc tcttcatttc ctgctcgcgt ttgttctctc cgatctcgct
      541 ttgtaagcga tcaatgtcgc gcttgttttc ccggatcctc ttttcggaat tctcgttggc
      601 gttttcgagc gacttgagtt cctgttcgta ggtgtccagg gtgcgctccg cctgtttcaa
      661 ttcgtcttcg accgcttccc ggtattgttg cacgccgaat tcccgcaacc aggagcggac
      721 cagcgccgcc ttggcatgtt ctccggtgaa gaaattctgg tcatcggtac caataaaaaa
      781 ttcgcccagg gtcgcgcctg cttcctcccg gaatcgggcg tagctgatga gcgaatcttc
      841 gccaagtcct ttcaccggtc cccggttcat gatccactcg ttaccttcga gcgatacgct
      901 gtttttagat tgactgcgca catatttttt ccatccgctc ccgagctctt tcggtttggc
      961 ttgtggaatg cgaatggcaa accccggctg ctcgccccgg ctcatgggtg aacggatgcc
     1021 caccacttgc agggagtctt gtgcccgcaa cgcggaagct gaaatcagca ttaaaacgag
     1081 tatcaaaagc cttttcatca tcctgcgaag atgccatttt tccccggaat ttgcgtgcta
     1141 aaggtcattg ctactctgcc gccggtcacg tattttagct aaccctgcgc aatacgaatc
     1201 ccaattcctg agagcatgaa aatcctgcga atccagttac tctggatcgg tttattcttt
     1261 tatcccttct tttccgtttg tcaggctcag caaacagctg atcctactag tggcatcctg
     1321 cctgattggg tgcggatgat ggacgatacg gccgttaact acaacgatgc cgtcaaagcc
     1381 ttcgaagcat tctggcaagg acgcgagaat ccgttggaag aaattgaaga acgggaaaaa
     1441 tatggctaca gcccggagcg cgaaggcgag atcagggaaa agttacgcga gctgagtccg
     1501 gaggaagctt cctggcgaca ggagatgatc tggcaatgca agcgtttcga agaatggcaa
     1561 cgcgatgtaa agccattcgt ccagaacgac ggtcgcatcc tgactccctc cgaacgcaag
     1621 gtcatggagg aagaacgcaa ccggcagttg aaggctgctg aaggaaagga gggtaagcga
     1681 tgacacgcgt ctcctgtgcg atcctcttcg taatcctgtt cgctttcacg tggacatccc
     1741 gcgcgcaaac agcatcctgg acaccgctgg cacccgcgct ttttccgacg aatgtaagcg
     1801 gacaaataca cggtatcagc cggatttccc agatgaaatt tcatccgtcc aattcatcca
     1861 agctgtacgc tgtgagcgca aggggcgggt tattcatctc ctccaacggc ggcacgagtt
     1921 ggagcctggc gcccggctgc gatgccatgc caaacggaac gcgcctggcg tctgtttgca
     1981 tcgatcatac gaacgatcag gtgctctatc tcggcggcgg tgatgcgaat tactactcca
     2041 ccggcagcgg agtctggaag tcgaccaatg gcggtaccac cttttcacag accggcctca
     2101 cgtccggagt ggtggtcgaa ctgctgatgg atccaaccaa caacaacatc ctggtcgcag
     2161 ctaccagcaa cggtatttac aagaccacca acggtggcac caactggacg ttaaagtcgg
     2221 gtacgatggc gttttacgat ctcgtgaaaa aggcgaatgc gagttcgcgt gtcttgttcg
     2281 cgaccggagc caatacgctg tggcgttcca ctgacttcgg t
//
LOCUS       DAOTON010000456         2320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_36009_length_2320_cov_3.372185, whole genome shotgun sequence.
ACCESSION   DAOTON010000456 DAOTON010000000
VERSION     DAOTON010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36009_length_2320_cov_3.372185"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1228)
                     /locus_tag="P5292_09070"
     CDS             complement(152..1228)
                     /locus_tag="P5292_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRS39316.1"
                     /translation="MEIKKLFIPGPTYVHPDVLAKMATPPIGHRTAEASALQRGISEK
                     LQQLMYTTNKIVLSTSSGTGLMEGAIRSLTQKKAIVFSVGAFGKRWWELARLNGIEAD
                     LHEEEPGHPTMPETVEHYLKTGQYDVVTVTHNETSTGIQNPIESIAEVVSKYPDVIWL
                     VDTVSSLGGVKVEVDRLGIDVCISSSQKALALPPGLALASISPKAEERLKAIGPRGYY
                     LDLGTLVKYVDEKDYQYSCTPSIAHMFALDFQLDRILKEGLENRFIRHVEMAEYMINW
                     ASHFYRVFPQPGFESMTVTCIENTRKTDIGALNKSLRERGMLISNGYGSLKDKTFRIG
                     HMGDLTLEDMEALTEAMEEILGLA"
     gene            complement(1449..>2320)
                     /locus_tag="P5292_09075"
     CDS             complement(1449..>2320)
                     /locus_tag="P5292_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39317.1"
                     /translation="SYFYSMSLGRISFLLCCWLVGQQAVKGQTARPFPLSADSVNSLV
                     KSLQRGPDDSTRAAANMIFRQHLQEYLKDSLSFSVPPYQGASNLGLVESEDKRFRLYS
                     WVAPSYAGDVYHFHGFLQVRDPKSGSISLFELSDSTTVILKPESEKLRANRWLGCVYY
                     QLVTVKKGSRTFYTLLGWKGKSERQTQKVIEVLYFEKNEPRFGYPLFRNEKVYSSRRV
                     FVFNSMASMALRYEPGKRMILFDHLSGGIDDPMSGPDGRYDGYRFKRGRWNLMKNVDI
                     RGDQKGGRKGVER"
BASE COUNT          568 a          592 c          555 g          605 t
ORIGIN      
        1 aactagtaca aattagttgt tagttattag ctattacaag atgcagctac tctattagtc
       61 tttccacagt tactactaaa gcttcactat tcgctattca ctattcacta ttcctttcat
      121 tgttcgctat tcgctattca ctattcacta ttcaggccaa ccccaaaatc tcctccatcg
      181 cttccgtaag cgcttccata tcctcgagtg tcaggtcgcc catgtgtccg atgcggaagg
      241 tcttgtcctt tagacttccg tagccattgg aaatgagcat gccgcgttca cgtaaggact
      301 tgttaagcgc gccgatgtcg gttttgcggg tattctcgat acaggtgacg gtcatcgatt
      361 caaagcctgg ctgaggaaac acccgataga agtgggaagc ccagttgatc atgtattcgg
      421 ccatctcgac atgccggatg aagcgattct ccagaccctc ttttagaatt cggtcaagtt
      481 gaaagtccaa cgcgaacatg tgtgcgatgg agggagtaca ggaatattga tagtccttct
      541 cgtcgacata cttcaccaag gtacccaggt ccaggtaata tcctcttgga ccgatagcct
      601 tcagacgctc ttccgctttc ggtgaaatgc tcgccaaagc caaacccggt ggaagagcca
      661 gtgctttctg tgaactggaa atgcaaacat cgattcctaa tcgatccact tcaaccttta
      721 caccaccgag cgaactgacg gtatcgacca gccagatcac atccggatac ttggagacaa
      781 cctctgcgat gctctcgatt ggattctgga tgccggtact ggtttcgtta tgggtaaccg
      841 tcaccacgtc atactggccg gttttgaggt aatgttcaac ggtctccggc atagtcggat
      901 gtccgggttc ttcctcgtgc aggtcggctt cgatgccgtt gagacgagca agttcccacc
      961 aacgcttgcc gaacgcccca accgagaata cgatcgcttt cttttgcgtc aaacttcgaa
     1021 tggccccttc catcaaaccg gtaccggaag aagtactcaa tacgatcttg ttggtggtgt
     1081 acatcaactg ctgcaacttt tcgctgatgc cgcgctgcaa tgcactggcc tccgccgtgc
     1141 ggtgcccgat aggcggtgtg gccatcttgg ccaggacgtc aggatgaacg taggtagggc
     1201 ccggtatgaa gagttttttg atttccattt ttaagctgga ccggctcggt cgagggtcgc
     1261 aaatttacgt gttttggagg gaagattcgg cgtaactgtt caaatgcgat tggcaaacgg
     1321 tgaagagtta atagtgaaca gtaaacagta aacagtagtg agtgaaattt tatcttcttg
     1381 tccgtttttg actaaaaacc ccatcacaag tcactaatta ctaattacta attactaatc
     1441 tccaatctct accgctcaac acccttccgc ccgcctttct ggtcgcctcg tatatctacg
     1501 tttttcatga ggttccatcg tccgcgtttg aatcggtagc cgtcgtatcg cccgtcgggt
     1561 cccgacatgg gatcatcgat gccgcctgac aaatgatcaa acagtatcat ccgtttccca
     1621 ggttcatagc gaagcgccat gctggccatg gagttgaaga cgaatacccg ccgactgctg
     1681 taaacttttt cgttacgaaa aagcgggtag ccgaaacgcg gttcgttctt ttcaaagtaa
     1741 agtacttcga tcactttttg tgtttgccgt tccgatttcc ctttccagcc caacagggta
     1801 tagaaagttc gcgagccttt cttcaccgtt acgagctgat agtaaacgca gcccagccag
     1861 cgattggctc tcagtttctc tgactcgggt ttaaggatca ccgtagtgga gtcggacagt
     1921 tcaaacaggg agatcgatcc cgacttcgga tcacgaacct gaaggaagcc atggaaatgg
     1981 taaacgtcac cggcatacga cggagctacc catgaataca gacgaaagcg cttgtcttcc
     2041 gactccacaa gtcccaggtt ggatgcaccc tgatagggcg gaactgaaaa gctgagagaa
     2101 tcctttaagt actcctgtaa atgctgacga aagatcatat tcgccgcagc ccgtgtgcta
     2161 tcatccggtc cgcgttgaag ggacttgaca aggctattta cggagtcggc cgatagggga
     2221 aagggtcggg cggtttgtcc cttgactgct tgttgtccaa ccaaccagca gcaaaggagg
     2281 aaagagattc gtcccagaga catggagtaa aaataagaac
//
LOCUS       DAOTON010000457         2320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_36013_length_2320_cov_2.985872, whole genome shotgun sequence.
ACCESSION   DAOTON010000457 DAOTON010000000
VERSION     DAOTON010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36013_length_2320_cov_2.985872"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="P5292_09080"
     CDS             <1..457
                     /locus_tag="P5292_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39318.1"
                     /translation="VPVVVHKKSGLSYMHMYTFFDREEHSSIIANSSLFRMRIPIEFV
                     SEKTDAPYGFGLEFQFWLDGSGECKDAQHIALIVRSDNTLQVFDKRIPYADNFVCGGW
                     NCWNGKKKMEYQDIGNYKQVATYSTDKGKPVVLEVIRNREEFYIYLNDQ"
     gene            935..1078
                     /locus_tag="P5292_09085"
                     /pseudo
     CDS             935..1078
                     /locus_tag="P5292_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006264397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            1259..1387
                     /locus_tag="P5292_09090"
     CDS             1259..1387
                     /locus_tag="P5292_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39319.1"
                     /translation="MSSVSQLAREIGLDEAVSGAQPSNVFLSALLKSFEEWKTSAR"
     gene            1474..2298
                     /locus_tag="P5292_09095"
     CDS             1474..2298
                     /locus_tag="P5292_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRS39320.1"
                     /translation="MPMLSEGRFLSTRARSNRIVWFVLLFQVATSIVSQAATNRIRTA
                     HSIGWYNTFLTFRLSDKSSIQAEYQWRREGWISNWQQSLLRIGYHVQLHPRVTARLGY
                     GWIETFPYGDIPLNGFGKQFTEHRLFQAIVLQDEVKRLSISQRWMLEERWLGRYASAA
                     STKEEDFDLLGRIRYQVRLQHPISKPGDGQGHFYIATYDELFIGFGKNVKENVFDQNR
                     LAGLIGFQSRSSFRVEAGFLQQLLQLGREVNGKNVFQYNNGVTVNLYYMLDLRKRN"
BASE COUNT          591 a          566 c          593 g          570 t
ORIGIN      
        1 agtgcctgtc gttgtgcata aaaaatcagg actgagttac atgcacatgt atactttttt
       61 cgatcgcgaa gaacattcaa gcatcattgc caattcctcc cttttcagaa tgcggattcc
      121 gatagaattc gtgtctgaaa agaccgatgc accgtacggc tttggactgg agtttcagtt
      181 ctggctcgac gggagtggcg aatgcaagga cgcgcagcac atcgccctga ttgtgaggag
      241 cgataacacc ctccaggtct ttgacaagcg aattccttat gcagacaact ttgtttgcgg
      301 tggatggaat tgctggaacg gaaagaaaaa gatggaatat caagatatcg gaaactacaa
      361 acaagttgcg acttattcga ctgacaaagg caaacctgtt gtgcttgagg tgatcagaaa
      421 ccgtgaagaa ttttacatct acctgaacga ccagtaagct taccaatcac ccatacccgc
      481 ttgcgatttc gggtatgggg atttcccaat cccgctattt tacaataaag gggagtttcg
      541 gtttttaccc ggtacagaat atacaattta cagacataaa agccgctagt cggaagatcc
      601 tcccacatcc ctatttcaag cgtgtttcac tttgtccaaa gcgtatcgga tagggagtta
      661 gaataaacaa aaatgtacag aacgtttggc aaaaaacctg tctgaaataa agttttctga
      721 tactatgctg ttcgaataac ggaacaagaa ctaacgtgta actttcatag atagattgaa
      781 cgagccgttt gaaatcctga gatggcatac tccttttgaa attctgtttt tcaactgaaa
      841 tgattttggt tgtccttact ggaaattctc ttaacccgaa tagaaaagga cccttccgtt
      901 accacccagg aagcaatcag ccatggaaca gaaaatgaaa gtggagatct ggtcggacgt
      961 gcaatgtccg ttctgctaca tcggcaaacg gcatttcgag caggcgatgg cacagttcgc
     1021 aaatgccgga cagatcgaag tggagtggca cagcttccag ctcgatccgg acctgcccaa
     1081 gccggcgagc cagctcaaca tctaccagta cctgggggtc ggtgtgggtg aagtggcggc
     1141 tgcaagcggg gaaggtgtga gtttagattt atttcactgc tcacccacac tgtttcttag
     1201 tcttgcactt aaatcccccc acaaagaaaa tccagtgtaa gtttgagaaa caatgtgagt
     1261 gagcagtgtt tctcaactgg cacgggagat cggactggac gaagccgtat caggcgcgca
     1321 accgtcgaat gttttcctgt cggctttgct gaagtcgttc gaggagtgga agacgtctgc
     1381 ccgctaattc ctttcgggtg cggtctgttg gagcaccact gattattcgt tgaccttcct
     1441 tccctgcgta atttcctttt aacttacgcg aatatgccca tgctttccga aggacgattc
     1501 ctttcaaccc gcgcacggtc taacaggatc gtgtggttcg tgctgctgtt tcaagtagcg
     1561 accagcatcg tttcgcaggc ggcgaccaat cgcatccgga cagcgcacag catcggctgg
     1621 tacaacactt ttctcacgtt tcgcctttcc gacaagagca gcatccaggc ggagtaccag
     1681 tggcggcggg agggatggat ctccaactgg caacagagcc tcttgcggat cgggtaccat
     1741 gttcagttgc acccgcgcgt gacagcgcgc ctgggttacg gttggatcga gacctttccc
     1801 tacggtgaca tcccactgaa tggttttgga aagcagttca ccgagcaccg cctgttccag
     1861 gccatcgtgc tgcaagacga agtaaagcgc ttgtcgatct cccaacgctg gatgctggaa
     1921 gaacgctggc tgggacggta tgccagtgcc gcgtccacga aggaggaaga cttcgacctg
     1981 ctcgggcgca ttcgctatca ggttcgctta caacatccga tcagcaagcc cggcgacggc
     2041 cagggccatt tctacatcgc cacctacgac gaactcttca tcggctttgg caagaacgtg
     2101 aaagaaaacg tgttcgatca gaaccgcctc gcgggtttga tcggctttca gtcacgttca
     2161 tcgttccgcg tcgaggcggg attcctgcag caattgctgc aactgggaag ggaagtgaac
     2221 ggtaagaatg tgtttcagta caacaatggg gttacggtga acctgtatta catgctggat
     2281 ctgcggaagc gaaactgacg cagcactctc cgatgttttt
//
LOCUS       DAOTON010000458         2318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_36059_length_2318_cov_2.566505, whole genome shotgun sequence.
ACCESSION   DAOTON010000458 DAOTON010000000
VERSION     DAOTON010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36059_length_2318_cov_2.566505"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="P5292_09100"
     CDS             <1..526
                     /locus_tag="P5292_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRS39321.1"
                     /translation="RLFLSLILLSVPMAYLGSLTVLPDRIYKVLLGCCLLISVVRIAI
                     PMGHSEHNATRLLPTWLACIIGGFIGLVSGMVGIGGGILLSPVLLLGRWSTIKETAAL
                     SAPFIFVNSLAGLSGLFLSDKLSVFPEPFWLIAAMTGGLLGAWSGSRKLPVPALRWVL
                     VVVLSVAAGKLFLP"
     gene            528..1736
                     /locus_tag="P5292_09105"
     CDS             528..1736
                     /locus_tag="P5292_09105"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRS39322.1"
                     /translation="MIRVEEARQLLLTIVRSMPAEVVSLRNAIGRVLAADIRAPLPMP
                     LFDQSAVDGYVLSSRRLPDGSYRVVGMIRAGDIPPKTIQSGQAYRIFTGAAVPQRAYG
                     VVMQEHVQCTGVRLDVPADRIVQGSHIRKKGGHFKKGAVVLQRSALLNAAAIGLLASL
                     GKQTVRVYRKPVIGLLVTGSELRKAGTVLREGEIYESNSSTLIAALESCGYSVKQLAF
                     ARDRMAEVERQAKRLMKVCDVVLFSGGISVGDFDFVLPVLGRLGVKQLFYKVAQKPGK
                     PLYAGSLGRKIVFALPGNPASALVCLYEYVLPTLDGMQMKPHRGLVQVGLPLASELSL
                     KADRDQFLRARVVHGHVEVFTAQDSDNLRSFAESNALVFVPAGVSRLSAGEQVIVHLL
                     PQQEIVASNS"
     gene            1741..1968
                     /locus_tag="P5292_09110"
     CDS             1741..1968
                     /locus_tag="P5292_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRS39323.1"
                     /translation="MRVRLFGMFAEALEVETVTVNSSGTVADIRKALLDFHPVFRQVP
                     FRIAVDRRLALENLQVSEDQEVAALPPFSGG"
     gene            1972..>2318
                     /locus_tag="P5292_09115"
     CDS             1972..>2318
                     /locus_tag="P5292_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRS39324.1"
                     /translation="MDQQYKDRYARQEALPEIGKAGQSRLARASVLVVGAGGLGCPAL
                     LYLAGAGLGRIGVVDHDQVAASNLPRQIVYREADLGRNKAEVAVQQLRQINSTLQYEA
                     IPEPLVAGNAKSL"
BASE COUNT          426 a          559 c          750 g          583 t
ORIGIN      
        1 gcgccttttt ctatcgctca tcctcttatc ggttccgatg gcttatctcg gttcgctgac
       61 cgtgttaccc gatcggatct acaaagtatt gctgggttgc tgtctgttga tctcggtcgt
      121 ccggatcgcg ataccgatgg ggcatagtga gcacaacgcg acgcgtctgt taccaacctg
      181 gcttgcgtgc atcatcggag gctttatcgg tcttgtgtcg ggaatggtcg gcataggcgg
      241 cgggatactg ttgtcgccgg tgttgttgct tggccgctgg tcgaccatca aggagaccgc
      301 tgcgctatcg gcgccgttca ttttcgtgaa ttcccttgcc gggctgagcg gattattcct
      361 cagtgataaa ttgagtgtat ttcccgagcc cttctggttg atcgctgcca tgaccggcgg
      421 actgttggga gcctggagcg gcagccggaa attgccggtg ccggccttgc gctgggtgtt
      481 ggtggtagta ttgtctgttg ccgccggtaa attgttcctt ccctgagatg atccgcgtag
      541 aagaggccag gcagttgtta ctaacgatcg ttcggtcgat gccggctgaa gttgtcagtc
      601 tgcggaacgc tatcggtcgt gtcttagcgg ctgatatacg cgcacccctc ccgatgccgt
      661 tgttcgatca gtcggcggtg gatggttatg tcttatcatc aaggcgtttg ccggatggaa
      721 gttaccgggt cgtggggatg attcgcgcag gagatattcc gccaaagacg attcagtccg
      781 ggcaggctta tcgcatcttt accggtgctg ctgttccgca acgggcctac ggcgtcgtga
      841 tgcaggagca cgtgcagtgt acaggtgttc ggttagacgt tccagcggat cgtatcgtgc
      901 agggttcaca cattcgaaag aagggtggtc atttcaagaa aggggcggtc gtgctgcagc
      961 ggtccgcgct gttgaatgcc gccgccatcg ggttactggc gtcgctggga aagcagactg
     1021 tgcgcgtcta tcgcaagcca gtgattggat tgctggtcac cggcagcgag ttgcgaaagg
     1081 cgggaacagt cttgcgggaa ggggagatct acgagtccaa ctcttcgacc ctcatcgctg
     1141 ccctggagtc gtgtggttat tccgtaaagc aattggcatt tgcccgtgac cgcatggcgg
     1201 aggttgagcg acaggcaaag cgcctgatga aagtgtgtga tgtggtgctt ttttccggtg
     1261 ggatctcggt gggggatttc gatttcgtat tgccggtcct cggacgcctt ggggtgaagc
     1321 aattattcta caaagtcgcc caaaagcccg gaaagccgct ctatgctgga agtttgggga
     1381 ggaagatcgt gtttgcacta cccggcaatc cagcatccgc cttggtttgc ctgtacgaat
     1441 atgtcctgcc aacgttggat gggatgcaaa tgaagccgca tcggggtctt gtgcaggtcg
     1501 ggttgccgtt ggcttcggaa ttatccctga aggcggaccg tgaccagttt ctccgggctc
     1561 gtgttgtgca tgggcatgtg gaggttttta cggcgcagga ttcggataac ctgcgtagct
     1621 tcgcggagag caatgccctg gtgtttgtac cggccggtgt gtcccggtta agtgccggag
     1681 agcaagtgat cgtgcacttg ctgccgcaac aggagattgt tgcatctaat tcgtgagacc
     1741 atgcgggttc gattattcgg aatgtttgca gaagcgttgg aagttgaaac tgttacggtg
     1801 aacagttccg gaaccgtggc agatatacgc aaggcgttgc tcgactttca tccggtgttt
     1861 cgccaggttc cgtttcgtat cgccgtcgac cgacgccttg ccttggaaaa cctacaagtc
     1921 agcgaagatc aggaggtggc cgcgttgccg ccattctcag gaggttgagt catggatcag
     1981 cagtacaagg atcgttatgc gcggcaggaa gccttgccgg agataggcaa ggcggggcag
     2041 tcgcgacttg cacgggccag tgttctggtt gttggagcag gcggactggg ttgtccggct
     2101 ttgctttacc tcgccggtgc agggcttggg cgtatcgggg tcgtggatca cgatcaggtg
     2161 gcagcgagca atttgccccg gcagatagtg taccgggaag cggatcttgg caggaacaaa
     2221 gcggaagttg cggttcagca gcttcgacag atcaatagca cgcttcaata cgaagcgatc
     2281 ccggagccgt tggttgccgg taacgcgaaa tcccttat
//
LOCUS       DAOTON010000459         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_36198_length_2312_cov_2.877714, whole genome shotgun sequence.
ACCESSION   DAOTON010000459 DAOTON010000000
VERSION     DAOTON010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36198_length_2312_cov_2.877714"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1958
                     /locus_tag="P5292_09120"
     CDS             <1..1958
                     /locus_tag="P5292_09120"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRS39325.1"
                     /translation="PAADKQEWALQFARLIRRNREYDQWSLTKKDQRNGMVYWGTLIG
                     LTDIKPPLRLSLTPYLSIYGENQTSLGENGQRTHDNSYSYSGGADLKYGIDERFTLDL
                     TLLPDFSQVQSDNKIKNIGAFEVIYDERRPFFKEGIELFSRGRLFYSRRVGGTPSGYY
                     DVPYLLNPGESIEENPDKARLVNAVKVSGRTNKGLGIGLFNAVTANTYATIRTADGGK
                     RKILTSPLTNYNILVFDQQLPNNSSSYFINTSVMRDGASRDADVSTGSYTFENKKHTV
                     RISSRYSRSEVRQWVDNPEGGRTKDKKDGSQFSFVADKISGTWQYGGVFEFGDKKFDK
                     NDLSYIFVNDYSDINLYGSYNKFNPFWKHFRQGNLNAWIYRGGRWSVNNLMTQFQGGL
                     NFWLLFNNNWSVYADGGSTFAEGRDYFEPRTYDGRYYVTPMNHWGSINLTTDYNKKLA
                     FDFGTRTWQAPSFGASSYGYYIVPMIRFSDRWSFKVNHNWDYTLNDRGFSTFNSVGNP
                     VFGRRDVLTITNSVTTRYLFKTDMSLSLIMRHYFSQGDYDRYYDLGNAGELVQNDGYL
                     NPSVEMLSTDFRANYFNVDLVYNWVFSPGSSLIITYKNQIAHEDSAILPGYFRNLSNT
                     IEEPQTNSISVKVLYFLDWEYLKRHKS"
     gene            2106..>2312
                     /locus_tag="P5292_09125"
     CDS             2106..>2312
                     /locus_tag="P5292_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS39326.1"
                     /translation="MISAKGITKRYEQLEILKGIDLEVARGEVVSIVGASGAGKSTLL
                     HILGTLDVPDAGTLTIAGEEVSRLK"
BASE COUNT          564 a          645 c          595 g          508 t
ORIGIN      
        1 tcccggcggc cgacaaacag gaatgggcat tgcaatttgc ccgtttgatc cggcgcaacc
       61 gggaatatga tcaatggtcg ctgaccaaga aagaccagcg gaacggtatg gtgtattggg
      121 gcaccctgat cggactgacc gatatcaaac ctcccctgcg actttccctt acaccctatc
      181 tgtccatcta cggcgagaac cagacgagcc tgggcgagaa cggacaacgc acccacgata
      241 acagctacag ttactccggc ggtgcggacc tcaagtacgg gatcgacgag cgcttcaccc
      301 tcgacctgac cctgcttccc gacttttcgc aggtacaaag cgacaacaag atcaagaaca
      361 tcggcgcttt cgaagtcatc tacgatgagc gacggccgtt cttcaaggaa gggatcgaac
      421 tcttctcgcg cgggcgactg ttttattccc gccgggtcgg tggtacaccc tcgggttact
      481 acgatgtacc ttacctgctg aacccgggtg agtcgataga agaaaatccg gacaaagccc
      541 gcctggtgaa tgccgtaaaa gtcagcggtc gcacgaacaa aggactcggc atcgggttgt
      601 tcaacgcggt taccgccaat acctatgcta cgatccggac ggctgacggc gggaaacgga
      661 agatcctcac ctctcccctc accaattaca acatcctggt cttcgaccag caactgccca
      721 acaactcgag ctcctacttc atcaacacca gcgtgatgcg cgacggcgct tcccgggatg
      781 ccgacgtatc gaccggttcc tacaccttcg aaaacaagaa gcacaccgta cgcatctcga
      841 gccgttattc gcgcagtgaa gtcaggcagt gggtggacaa tccggaaggc ggacgaacga
      901 aggataagaa ggacggcagc cagttcagct tcgttgccga caagatcagc ggaacctggc
      961 agtatggcgg cgtgttcgaa ttcggcgata agaaattcga caagaacgac ctgtcgtaca
     1021 tcttcgtaaa cgattacagc gacatcaacc tgtacggcag ttacaacaaa ttcaacccgt
     1081 tctggaaaca tttccgtcag ggcaacctga acgcctggat ttaccggggt ggtcgctgga
     1141 gcgtgaacaa cctgatgacg cagtttcagg gcggcttgaa tttctggcta ctcttcaata
     1201 acaactggtc ggtttatgcc gatggtggca gcactttcgc cgaggggcgt gactatttcg
     1261 agcctcgcac ctacgacgga cgttactatg tgaccccgat gaaccattgg ggaagcatca
     1321 atttaactac cgattacaac aagaagctgg cgttcgactt cggaacccga acctggcagg
     1381 ctccttcctt tggcgcttcc agctacggtt attatatcgt tccgatgatc cggttcagcg
     1441 atcgctggtc cttcaaagtg aaccacaact gggattatac cctgaacgac cggggctttt
     1501 cgaccttcaa cagtgtcggc aatcctgttt tcggcagacg tgacgtcctg acgatcacca
     1561 attcggttac gacccgttat ctgttcaaga ccgacatgtc gctctcactc atcatgcgtc
     1621 attacttctc acaaggtgac tacgaccggt actatgatct cggtaacgca ggagaacttg
     1681 tccagaatga tgggtacctc aacccgtcgg tggaaatgct atcgaccgat ttccgcgcca
     1741 actacttcaa cgtggacctg gtctataact gggtgttctc tcccggcagt tcgctgatca
     1801 ttacctacaa gaatcagatc gctcacgaag actcggccat cctacccggc tacttccgca
     1861 acctctccaa caccatcgaa gaaccgcaaa ccaattcgat ctcggtgaag gtgctttact
     1921 ttttggattg ggagtatttg aagaggcaca agagttagag ggtttcgggt ttcgggtttc
     1981 cggtttccgg tttccggttg gttaacgtta aaacgtttaa acgtttaaac gttcgaacgt
     2041 tttaacgaac tcggaactcg aaacccggaa caagataccc catcctttcg ctatcttcgc
     2101 aatcgatgat cagcgcgaag ggtatcacga aacgctacga gcagttggag atcctcaagg
     2161 gcattgacct ggaggtggcc cggggcgaag tggtgagtat tgtcggcgct tcgggagcgg
     2221 gaaaatccac cctcctgcat atcctgggca cgttggacgt tccggatgcc ggaaccctga
     2281 cgattgccgg agaagaagtc agtcgcctga ag
//
LOCUS       DAOTON010000460         2303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_36389_length_2303_cov_2.538701, whole genome shotgun sequence.
ACCESSION   DAOTON010000460 DAOTON010000000
VERSION     DAOTON010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36389_length_2303_cov_2.538701"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="P5292_09130"
     CDS             complement(<1..1568)
                     /locus_tag="P5292_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39327.1"
                     /translation="MIQRNVKILLTALLAMLLSASTTAQIANVKGLYVNFINSWLGNT
                     VEEDKILDYCQVNGFNYITLYDLNLLNWSATQKTQLAAFITKAKNVYGVQQIGASGET
                     SNFFRNYIVPYNTGRTNAIERFNVFNFEFEFWLTASIASYYGPVYLTPNGFTSDTAGA
                     FAFARREYARIDSMATVYGILNEIYLGWPNTGQMQQLVTYSDRILLHAYRPTDVDVYP
                     YTQSRIYDIVSAGRPVQIMPIFSAETSFMGPWLTNNPITKPYQTFSSGYGLESANVKQ
                     HANLQGYQWFLYSLMPKTSLVNASISTSGPTTFCSGSSVILTASTGATYLWSPGGQTT
                     RSITVNAAGSYSVTVTGAAGNSATSAPVVVTVNAAPTAPTITANGPTSFCSGSSVILV
                     ASATSNYRWSNGATTQAISVSSTGSYSVSTTNNLCTSTSTPVTVVASPVPAVPTISVI
                     GSPSLCPGATVTLTSSASNGYLWSNGATTRSIVVSAAGTYSVRAYSGPSCYRASATIT
                     TTALTGPATPVISL"
     gene            1962..>2303
                     /locus_tag="P5292_09135"
     CDS             1962..>2303
                     /locus_tag="P5292_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39328.1"
                     /translation="MKSSSFYHGLLFFFSMAISSMALAQQDSTARLGLPGDNLNLAAV
                     LDVFRQSPTLESFESALNADTSKINNLDLNNDGKVDYIKVVDRPEENVHTIVLQVDLN
                     EKETQDVAVIFV"
BASE COUNT          541 a          542 c          676 g          544 t
ORIGIN      
        1 tgcagggaaa tcacaggagt tgcgggaccg gtcagggcag tcgtcgtaat ggtagccgaa
       61 gcccggtagc aggaaggacc ggaataggca cgcacactat acgtgccggc agcgctgact
      121 acgatggaac gcgtggtagc gccgttggac cagagatacc cgttggaagc ggaactggtc
      181 agggtaacgg tggctcccgg acacaaactg ggtgaaccga tgacggaaat cgtcggtact
      241 gccggtacgg gagaagcgac cacagtgacc ggcgttgatg ttgacgtaca taaattgttc
      301 gtcgtgctga cggaatagct tccggtcgag gataccgaaa ttgcttgtgt agtggcgcca
      361 ttcgaccagc ggtaattgga cgttgcggac gcgaccagga tgacactgct gccgctgcag
      421 aagctggtcg gcccattcgc cgtaatcgta ggcgccgtgg gtgctgcgtt aacggttacc
      481 acgacagggg cggaggtcgc gctgttaccg gcggctccgg tgacggtgac cgaataggag
      541 ccggcagcgt tgaccgtgat agaacgcgtg gtctgtccgc ccggactcca caagtaggtt
      601 gcaccggtgc tggcggtaag tattacactg cttcccgagc aaaaggtggt tgggccgctc
      661 gtactgatcg acgcgttcac gagcgaagtt ttcggcatga gtgaatacag gaaccattgg
      721 taaccctgca ggttcgcgtg ctgtttgaca ttcgccgatt cgagaccgta gccggaagaa
      781 aaggtctgat agggtttcgt gatcggattg ttcgtaagcc agggacccat gaagctggtc
      841 tccgcggaaa agatcggcat gatttgcacc ggccgtcctg cgctgacaat gtcgtagata
      901 cggctttgcg tatagggata gacgtcgaca tcggtaggcc ggtaggcgtg caggaggatg
      961 cggtcggaat acgtgaccag ttgctgcatc tgtccggtgt tcggccagcc gaggtagatt
     1021 tcattcagga tgccgtaaac ggtagccatg gaatcgatcc tggcgtactc ccgtcgggcg
     1081 aatgcgaaag ccccggcggt gtcggaagtg aatccgttcg gcgtcaggta aaccgggccg
     1141 tagtaactgg caatactggc ggtgagccag aattcgaatt cgaaattgaa aacattgaat
     1201 cgctcgatgg cgttggtcct tccggtattg taggggacga tgtaattgcg aaagaagttc
     1261 gatgtttcac cggaagcgcc gatctgctgg acgccgtaaa cattcttcgc cttggtaatg
     1321 aaagcggcga gttgcgtctt ttgggtagcc gaccagttca gcaggttcag gtcgtacagg
     1381 gtgatgtaat tgaagccgtt cacctggcag taatcgagga tcttgtcttc ctccaccgta
     1441 ttgccgagcc aggaattgat gaagttgacg tagagacctt tgacattcgc gatctgtgcg
     1501 gtggtggatg cggagaggag cattgccaac aaagccgtta gcaggatctt tacgtttcgt
     1561 tgaatcatcg agtcgttgtt ttgagtgagt aattcttcgg gcagaactaa atgggctcag
     1621 agaaatttca ccagttgttt acgcctcaaa atgcacatac gattcggttg gaagtttccg
     1681 ccggcgaatc gttattaaca ccgttgttaa ttcatttcta ttccaaaatc cccggaagcc
     1741 accagtattc gcgactttca gcagggtgca ttttagtggg aggtgaaaaa atgtggaaaa
     1801 ccctacctta aatttgatgg aatcgaaaac catgaaccct taatctgtcg ctcagtagaa
     1861 tctggcctga tccttgtcat tcagaatttt gcttaggatg tgtagcatta cgctgattat
     1921 ccaagccgtc catctcatca tcacttaaaa atacctgaac catgaaaagt tcgagtttct
     1981 atcacggcct gttgttcttc ttctcgatgg ccatttcatc aatggccctg gctcaacagg
     2041 atagtaccgc gcggctgggt cttccgggtg acaacctgaa cctcgcggct gtgttggatg
     2101 tattccgcca atcaccgacc ctcgaatcat tcgaatcggc cttgaacgcg gacaccagta
     2161 agatcaacaa ccttgatctg aacaatgacg gaaaggtcga ctatatcaaa gtggtggacc
     2221 gcccggaaga gaatgtccat accatcgtgc ttcaggttga cctgaatgaa aaggaaacgc
     2281 aggatgtcgc tgttatcttt gtg
//
LOCUS       DAOTON010000461         2300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_36447_length_2300_cov_3.483296, whole genome shotgun sequence.
ACCESSION   DAOTON010000461 DAOTON010000000
VERSION     DAOTON010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36447_length_2300_cov_3.483296"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="P5292_09140"
     CDS             complement(<1..324)
                     /locus_tag="P5292_09140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS39329.1"
                     /translation="MKPIRISVLLPFYHEGSLLEEAMESVRHQHCDGWELVLVDSQAD
                     DRTRSIAARYAISDPRIRMVPANAPGISAALNTGLSECAGAYIARMDADDRMHPERLL
                     KQADFL"
     gene            complement(498..1163)
                     /locus_tag="P5292_09145"
     CDS             complement(498..1163)
                     /locus_tag="P5292_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS39330.1"
                     /translation="MNEVKKKILVVDDEVRLRENICELLDLNGFLTAAAENGQQAEQQ
                     VREFQPDLVICDIRMPLMDGYEFLQRLRENTERKDIPLIFISAKVERDDIRKGMNLGA
                     DDYLTKPFMKDELLQAVTTRLKRKEQMSAGHQTDVQNAKLQYTREEVMQHLRKLTKSE
                     RHILRRVAEGKTSRQISEELFISFKTVENHRSNISEKLGISGTLSLITYAFSIKPFLD
                     SIE"
     gene            complement(1160..>2300)
                     /locus_tag="P5292_09150"
     CDS             complement(1160..>2300)
                     /locus_tag="P5292_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS39331.1"
                     /translation="GMICICRDISKRRERDEEMQKLALVSNRSNNIILILDNQRRVDW
                     VNDSFLRLTGRTKEEVLGTRFPSETPGWSNDNLPPWLQFGMHQHQHMKGELAYYTATG
                     EEIWLQGEINPVYDDNGEFRYSACILNDITLIKAAEKNALTALHKERALSELKSRFVS
                     LASHEFRTPLAGILSSTDIIREYADTIEDEAAARILNHTDRIAEEVLRMTEIMNNVLL
                     LGKMDAGKIPFRPGSGDIHEFIQTHLRRVSTAFRERVRFNGPGRPVHTRFDPNLLDHI
                     LSNLLSNAAKYSPAELDIIVDLLPDEADGIQIVVRDKGIGIPAKELPFLYESFFRGSN
                     TNDFHGTGLGLSLVKQFVDLHGWFIRIQSEENNGTDVTIIIPTES"
BASE COUNT          446 a          598 c          671 g          585 t
ORIGIN      
        1 gaggaagtcc gcttgtttca agagacgctc gggatgcatc cggtcgtcgg cgtccatgcg
       61 cgcgatgtaa gcgccggcac attcggaaag accggtgttc agggctgcac tgatccccgg
      121 cgcgttcgcg gggaccatgc ggatccttgg gtcggatatt gcgtatcggg ctgcgatgga
      181 acgcgtccgg tcatccgcct gactgtcgac cagaaccaat tcccatccat cacaatgctg
      241 atgacgtacg ctctccatgg cttcctccag cagggaacct tcgtgataaa acggcagcag
      301 gacggaaatt ctgatgggtt tcacggccga aaaggtacgg gcttgcaggg ggaataaatg
      361 cgaaccccgg catcttgcgg gatgccgggg ctgcaaccaa gtaactcatt caatcggaac
      421 tgaagttcga attgcactgt aagatctctg gtcggttacg tatttccaat ggagcgggat
      481 aaaatgaggg aacaccctta ctcgatggaa tcgaggaatg gtttgatgga aaacgcgtag
      541 gtgatcagcg aaagcgttcc gctgataccg agtttttcgg atatgttgga ccggtgattt
      601 tccacggtct tgaagctgat gaagagttct tcggagatct gcctgctggt cttgccttcg
      661 gccactcttc gcaggatgtg ccgttcggat tttgtgagtt ttcggaggtg ctgcatgacc
      721 tcttcacgcg tgtactggag tttggcgttc tgcacatcgg tctgatgacc cgcactcatc
      781 tgttctttcc ttttcaggcg tgtcgtgacc gcttgcagga gctcatcttt catgaacggt
      841 ttggtcaggt agtcgtcggc gcccaggttc atgcctttcc ggatgtcgtc gcgttcgacc
      901 ttcgcggaga tgaagatcaa cgggatgtct tttcgttccg tgttttcgcg tagtcgttgc
      961 aggaattcgt atccgtccat cagcggcatc cggatgtcgc agatcacgag gtcgggttga
     1021 aattcccgga cctgctgctc ggcttgctgt ccgttctcag ccgcggctgt caggaagccg
     1081 ttcaggtcga gcagctcgca aatattctcc cgtaatctga cttcatcgtc gacgaccagg
     1141 atcttcttct tcacttcatt catgactcgg tagggataat aatagtaacg tcggttccgt
     1201 tgttctcttc actttgtatg cggatgaacc atccgtgcag atccacgaat tgtttgacca
     1261 gggaaagacc cagcccggtt ccgtgaaagt cgttggtgtt ggagccgcgg aagaaagatt
     1321 cgtacaggaa cggcagttcc ttggcgggaa tgccgatgcc tttgtctcgg accacgatct
     1381 gaattccatc ggcttcgtcc ggcaggagat cgacgattat gtccagttca gccggactgt
     1441 acttggccgc gttggacaac aggttgctga ggatgtggtc gagaagattc gggtcgaagc
     1501 gggtgtggac cgggcgtccg gggccattga agcggacgcg ttcacgaaac gcggtactga
     1561 ctcttcgcag atgggtctga atgaactcgt gtatatcgcc cgagcccggc ctgaatggga
     1621 tcttgcctgc atccattttc cccagcaaca aaacattgtt catgatctcc gtcatgcgta
     1681 acacttcttc ggcgatccgg tcggtatggt taagaatacg cgcggctgcc tcatcctcga
     1741 tcgtgtcggc gtattcacgg atgatgtcgg tactggacag gatgccggcg agtggcgtac
     1801 ggaattcgtg cgaggcaagg ctgacaaacc gcgatttgag ttcactcagc gcgcgctcct
     1861 tgtgaagggc cgtgagcgcg ttcttttcgg ctgccttgat gagggtaata tcgttcagga
     1921 tgcacgcgct gtagcgaaac tccccgttat cgtcgtacac cgggttgatc tcaccctgta
     1981 gccatatttc ttccccggtt gcggtgtaat aagccagctc gcctttcatg tgttggtgct
     2041 ggtgcatacc gaattgcagc cagggcggca ggttgtcgtt gctccagccc ggtgtttcgg
     2101 agggaaagcg cgtgccgagc acttcctcct tggtgcgccc cgtcagacgc aggaagctat
     2161 cgttgaccca atcgactctt cgctgattgt ccaggatgag gatgatattg ttcgagcggt
     2221 tggataccag ggccagcttc tgcatctcct catcccgttc ccgtcgctta ctgatgtcac
     2281 gacagatgca gatcatcccg
//
LOCUS       DAOTON010000462         2299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_36466_length_2299_cov_3.297237, whole genome shotgun sequence.
ACCESSION   DAOTON010000462 DAOTON010000000
VERSION     DAOTON010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36466_length_2299_cov_3.297237"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..1192
                     /locus_tag="P5292_09155"
     CDS             449..1192
                     /locus_tag="P5292_09155"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRS39332.1"
                     /translation="MRTLLSLVLLSCLAMIAPASSNAQLLVTFPAADSTTVTADWYPV
                     SDEFPTILLCHQNRFSRGEYQETALRLNKFGFNCLAIDQRLGSEVNGVINQTALEAKA
                     KGQQPEFPDAEQDIRAGLDFLYDKYNKPVLILGSSYSASLALKIANGNNKVSGVIVFS
                     PGEYFQDKDFTRNSINGLDKPLFATSSREESDKVTDLLRDVNSRIKVQYVPKTAGDHG
                     SKVLWSKSSDNQDYWAALMSFLMRMKKMQ"
     gene            1221..1964
                     /locus_tag="P5292_09160"
     CDS             1221..1964
                     /locus_tag="P5292_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39333.1"
                     /translation="MRSTMIHQKFLPFVLLFCLCLSTAYAQKNVTTVGLQFKPIFPLS
                     FLGTGTQSQLEQGVQFDFSLKSGFAAGMVVRRGMTDLLSFETGINYVKRTFTLDITDA
                     NFNTSSDFRMIAYEIPASLLAYIQLGEKLYMNASMGASADMFASSLETKGEGYEHRSK
                     RKNVLQPAVIANIGWEYRTVKSGYWYLGASYHRPFNYIYNSQVQYQRAGKDFIVEQRL
                     LGNYLTLDLRYFFHEDPDNLKRKRVYYEN"
     gene            complement(1961..2290)
                     /locus_tag="P5292_09165"
     CDS             complement(1961..2290)
                     /locus_tag="P5292_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRS39334.1"
                     /translation="MTLSQAQTTVDNWIRTHGVRYFSELTNTAILMEEVGEVARIMAR
                     KYGDQSMKPSDEGKELADELADVMFVLVCLANQTGINLEEAFQKNLEKKTARDQDRHK
                     NNPKLNG"
BASE COUNT          560 a          612 c          597 g          530 t
ORIGIN      
        1 ggggtggata ggagaaacta gtatttagta attattaact aggaactagt aactagtaat
       61 tgggggatag gggatggggg atgggggatg gggaatggag aatggagact gaggactgag
      121 gactgaggac tggaaacagt ggcctggggt tgctgtttaa atgttctact acctaaagtg
      181 gttaatttta ccgccgactt attataggga cgagggacga gggacgtggg acgagggact
      241 agggacgtgg gacgtgggac gtgggacgag ggacgaggag cgatgcatgc ctgccgaagc
      301 tttagcgaaa gcaggggcga ggcaatgaat gcctactgcc tactgcctac cgcaaaccga
      361 ttcccgccta ctgccttccg tttaccgctt agtcaacgaa gacagaaacg acagcctttt
      421 cgtatcttag tcgggttatt ctccacttat gcgaacactc ctatcgcttg tcttgctaag
      481 ctgtcttgcc atgatcgccc ctgcttccag caatgcgcaa ctgttggtga cctttcctgc
      541 tgcggacagt acgacggtaa cagccgattg gtatccggtg agcgatgagt ttccgaccat
      601 tctgctttgt caccagaacc ggttcagccg gggtgaatac caggaaacgg cacttcgtct
      661 gaacaaattc ggctttaact gcctggccat cgaccaacgt ttaggcagcg aggtgaatgg
      721 cgtgatcaac cagaccgccc tggaggcgaa agcaaaaggc cagcaaccgg agtttccgga
      781 cgctgaacaa gacatccgcg caggactcga ctttctctat gacaaataca acaagcccgt
      841 cctgatactc ggcagctcgt attcggcttc gcttgcgctt aaaatcgcga acggcaacaa
      901 caaagtatcc ggcgtgatcg ttttcagtcc gggcgagtat ttccaggaca aggatttcac
      961 ccgcaactcc atcaacggac tggacaagcc gctcttcgcg acctcctcgc gggaagaatc
     1021 cgacaaagta accgacctct tgcgcgacgt gaattccagg atcaaagtac agtacgtacc
     1081 gaaaaccgcc ggcgaccatg gatccaaagt actttggtcg aagtcgtccg acaatcagga
     1141 ttactgggca gcgctcatga gcttcctgat gcgcatgaaa aaaatgcaat aagcctggca
     1201 agcagggaaa tctctgacgt gtgcgttcga ctatgattca tcagaagttc ctgccgttcg
     1261 tcctgttgtt ttgcctgtgc ctctcaacag cctatgcaca gaaaaacgtc accaccgtag
     1321 ggctgcaatt caaaccgatc ttcccgcttt ccttcctggg caccggcacc caaagccaat
     1381 tggagcaggg tgtgcaattc gacttttcgc tcaagagcgg attcgccgct ggcatggtgg
     1441 ttcgtcgggg catgacggac ctgctttcat tcgaaacagg catcaactac gtcaagcgaa
     1501 catttacgct tgacattacc gatgccaact tcaatacatc gagcgatttc cggatgatcg
     1561 cctatgaaat accggcctcg ctcctggcct acatccagct tggcgaaaag ctatacatga
     1621 acgcatccat gggcgcttcc gccgacatgt tcgccagcag tctggagacc aaaggggaag
     1681 gatacgagca tcgatccaag cgaaaaaatg tcctgcagcc agccgtcatc gccaacatcg
     1741 gctgggaata ccgcaccgtg aaatccggct actggtacct cggcgcgagc tatcatcgcc
     1801 cgttcaatta catttataat tctcaggtac agtaccagcg cgccggcaag gatttcatcg
     1861 tcgaacaacg cctgctcggc aattatctga cactggacct tcgttacttt tttcacgaag
     1921 accccgacaa cctgaaacgc aagcgggtct actacgaaaa ttaaccgttc aacttgggat
     1981 tgttcttgtg gcgatcctgg tcacgagcgg tctttttctc cagattcttt tgaaatgctt
     2041 cttccaggtt aatgcccgtt tgattcgcca ggcaaaccaa cacgaacatc acatccgcca
     2101 attcatccgc cagctctttt ccctcgtcgg aaggcttcat ggattgatcg ccatacttcc
     2161 gggccatgat cctggccacc tcgcctacct cttccatcag tatcgccgta ttcgtgagct
     2221 cgctgaaata acgtacacca tgcgtgcgga tccagttgtc gacggtggtt tgggcttggg
     2281 agagggtcat gggggtatt
//
LOCUS       DAOTON010000463         2297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_36505_length_2297_cov_3.301963, whole genome shotgun sequence.
ACCESSION   DAOTON010000463 DAOTON010000000
VERSION     DAOTON010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36505_length_2297_cov_3.301963"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="P5292_09170"
     CDS             complement(<1..850)
                     /locus_tag="P5292_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS39335.1"
                     /translation="MSTHRQLENQWIANFRRGPLVDADSGKLRIRIAHQLFYGRETED
                     YPADLPENFRAFVGEHAELQRTEAVRQLLAFLQPAGISLDYERVLRHLELQPDDFLKL
                     VKRTRYILQTLAHARDDFDTANPFRELGGDLAFLQRLASGEAASQLHLFEELDLPDLY
                     NRLGTLPPGYGNDPLIRQLFTRIEGGNESFFLTGKAGTGKSTFLHYFAQQTKKKVVIT
                     AFTGIAAVNVGGQTLHSFFLFPLKPLLPEDHEIRRFEEHSDRYKIIREMDTLVIDEVS
                     MLRADLL"
     gene            complement(1015..1548)
                     /locus_tag="P5292_09175"
     CDS             complement(1015..1548)
                     /locus_tag="P5292_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRS39336.1"
                     /translation="MKNSPKLPRILKVLSVRNHQVHCVFNNGETRRIDFIKLFSKWKV
                     GKKDPEYPLLDSRVFNKVRVHNQTLAWDNIRLEIPGPDGQPVEVPYEIDPDILYKNSD
                     PLNEPAHRFFFGSVIRQLREASGLSQEELALRSGTSKSYLSRVENDTIEPGLTTLYKI
                     IEIGLGGKLSIEVKKRR"
     gene            complement(1545..1790)
                     /locus_tag="P5292_09180"
     CDS             complement(1545..1790)
                     /locus_tag="P5292_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRS39337.1"
                     /translation="MPVIHQLGAIRILVYSRDHAPPHFHAVVAEYEALIEIKTLKVIA
                     GHLPKRQLQTVIRWARQPHNNQRLLETFHVLNPVKRK"
     gene            complement(1937..>2297)
                     /locus_tag="P5292_09185"
     CDS             complement(1937..>2297)
                     /locus_tag="P5292_09185"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HRS39338.1"
                     /translation="MVYTAMNRSLAMAEVAVHLSLAMLPEDYRMITIDIPDRVSRHQL
                     APNRLPAGWKAFPHPAATRAIGDRFIAEGKHCILIVPSAITQGDFNYLLNPRHPDFKK
                     IRIRKVEAFPFDRRMVR"
BASE COUNT          481 a          599 c          659 g          558 t
ORIGIN      
        1 ccagcaggtc ggcccgcagc atggatacct cgtcgatgac cagcgtatcc atttcccgga
       61 tgatcttgta ccggtcggag tgttcttcaa accgtctgat ctcatggtcc tccgggagca
      121 gcggtttcaa cgggaacagg aagaacgaat gcagggtttg tccgccgaca ttcacggcag
      181 caatacccgt gaaagcggtg atgaccacct tctttttggt ctgctgggcg aagtagtgca
      241 ggaaagtgga tttcccggtg ccggccttgc cggtcaggaa gaaactctcg ttgccgcctt
      301 cgatgcgggt aaaaagctgc cggatgagcg gatcgtttcc gtatccggga ggaagtgtcc
      361 cgagccggtt gtagaggtcg ggcaggtcga gctcttcgaa gaggtgcagc tgactcgcag
      421 cttcaccgct ggcgagtcgc tggagaaagg ccaggtcgcc gcccagttcg cggaacgggt
      481 tggcggtatc gaaatcgtcg cgggcgtgcg cgagggtctg gaggatatac cgggtccgtt
      541 ttacgagttt taaaaaatcg tccggttgta attccaggtg tcggagcacc cgttcgtaat
      601 cgagggagat gccggcaggc tgcagaaaag ccaggagttg gcggacggcc tcggttcgtt
      661 gcagctcggc atgctctcct acgaacgccc ggaaattctc gggcaggtcg gcgggataat
      721 cctccgtttc tcttccgtaa aacaactgat gggcaatgcg tatcctcaac ttcccggaat
      781 ccgcatccac gagcgggccg cggcggaagt tggcgatcca ttgattttcg agttgacggt
      841 gcgtggacat acttcggctg aacgaaagtt acaaggaagc cggtgccaat gcaagcactg
      901 tgggttgggt gcctggagtt tttcagtttg cagcgggcag ttcgcagtag gcagctcgag
      961 ttcgatgcct tatgctcaca tctcgatcct gatcccaatt tcaacgtaca gcagtcatct
     1021 tcgcttcttc acctcgatcg acagcttccc gcctaacccg atttcgatga tcttgtataa
     1081 ggtcgtcagc cctggctcta tcgtgtcatt ctccacacga gagagataag atttactcgt
     1141 cccgctccgt aatgccagtt cctcctgcga caatccgctt gcttcgcgta attgccttat
     1201 cacactaccg aagaagaagc ggtgtgccgg ttcattcaac ggatcactgt tcttgtacaa
     1261 aatatccgga tcgatttcgt atggaacctc tacgggttga ccatccggcc caggaatttc
     1321 gagccgaatg ttatcccagg cgagcgtctg gttatgtacc cggaccttgt tgaataccct
     1381 ggaatcgagt agcggatatt ccgggtcctt cttgcccacc ttccacttgg aaaagagttt
     1441 gataaaatcg atccgccggg tctctccatt gttgaacacg cagtgaacct ggtgattacg
     1501 gacagaaagc accttcagga tacgcggaag ttttggggag tttttcattt tctttttacc
     1561 ggattaagga cgtgaaacgt ttccaggagc cgttgattgt tatgcggctg gcgggcccag
     1621 cggatgacgg tttgcaattg ccttttgggc agatgaccgg ctattacttt taacgtcttg
     1681 atttcgatca gcgcctcgta ctcagcaaca accgcatgga agtgcggcgg agcatggtcc
     1741 cgggagtaaa cgagtattcg gatcgcccct agttgatgga tgacaggcat aagacgaaaa
     1801 gtcagcaatc aaagttacca tatttggtaa caactgtcaa gactttgcgc tgaatattcg
     1861 gttgcttcat ttcaatggac gattcgtagc atgcggctcc tcctcgccct tcgtccctcg
     1921 tccctcgtcc ctcgccttac cgcaccatcc tcctgtcaaa cggaaacgcc tccaccttcc
     1981 ggatgcggat cttcttgaaa tcgggatgac gtgggttgag aagatagttg aagtcgccct
     2041 gggtgatggc tgagggaacg atcaggatgc agtgtttgcc ctcggcaatg aaacgatctc
     2101 cgatcgcgcg cgtcgcggcg ggatgcggaa atgctttcca gccggccggg agtctgtttg
     2161 gcgcgagttg gtgacgggat acacggtcgg ggatgtcgat cgtgatcatg cggtaatctt
     2221 cgggcagcat ggccagactg aggtgcacgg ccacctcggc catggccagt gaacggttca
     2281 tcgccgtata gaccatt
//
LOCUS       DAOTON010000464         2291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_36624_length_2291_cov_4.452594, whole genome shotgun sequence.
ACCESSION   DAOTON010000464 DAOTON010000000
VERSION     DAOTON010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36624_length_2291_cov_4.452594"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="P5292_09190"
     CDS             complement(<1..820)
                     /locus_tag="P5292_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRS39339.1"
                     /translation="MLHLQNYINGVLVPPASGQYLDNFNPALGEVYSLIPDSDERDVA
                     AAQAAAQAAFPAWSTMPKEKRSALLLKLSGLIQKNLDRLALAESVDQGKPVWLAKSVD
                     IPRAVSNFHFYATGILHDSTEAHAMEETAINYTLRTPVGVAGCISPWNLPLYLFTWKI
                     APALASGCTVVAKPSEITPMTAYLLSELAIEAGLPPGVLNIVHGLGPKVGTAIVSHPD
                     IPAISFTGGTKTGETISSIAAPMFKKLSLELGGKNPTIVFADADFEKSVHTAVRA"
     gene            1119..>2291
                     /locus_tag="P5292_09195"
     CDS             1119..>2291
                     /locus_tag="P5292_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRS39340.1"
                     /translation="MSYPFQHTDRPAYDAAYRRSVQDPEGFWADVAANFYWRKRWEKT
                     LEWNFRDPDVKWFSGGKLNITENCLDRHLGTLGNKPAIIWEPNDPEEHHRVLTYRDLH
                     TKVVQFANVLKNNGVKKGDRVCIYMGMVPELAIAVLACARIGAIHSVVFGGFSAQSIA
                     DRLTDAQAEFIVTSDGAFRGNKEIGLKSVIDDALVQCRFVKRVIVLTRTRTPVSMIKG
                     RDVWWEDEIRKVETMGNPDCPAEEMDAEDPLFILYTSGSTGKPKGVVHSSAGYMVYTT
                     YSFANVFQYRPGEIFFCTADIGWITGHSYIVYGPLGAGATSLMFEGVPTWPDAGRFWD
                     IVDKYHVNILYTAPTAIRSLMGYGLGPLQGKHLNSLRVLGTVGEPINEEAWNWYHEN"
BASE COUNT          522 a          592 c          680 g          497 t
ORIGIN      
        1 ccgcacgcac ggccgtgtga accgattttt caaagtcggc atcggcgaat acgatcgtcg
       61 ggttcttgcc gcccaattcg agagaaagtt tcttgaacat gggagcggca atgctggaaa
      121 tggtctctcc ggtcttcgtg ccgcctgtaa acgagatggc gggaatatca gggtgcgaca
      181 cgatggcggt gccgaccttg ggccccagtc cgtgtacgat gttgagcacg ccgggaggta
      241 gtccggcttc gatggccagc tccgaaagga gataagccgt catcggcgta atctcggatg
      301 gcttggccac gaccgtgcat cccgatgcaa gcgccggggc gatcttccag gtgaagaggt
      361 agagcggcag gttccagggc gagatacaac cggcgacacc gacgggtgtc cgcaaggtgt
      421 agttgatcgc cgtctcttcc atcgcgtgag cctccgttga atcgtgcagg atgccggtgg
      481 cataaaaatg gaaattggaa accgcacggg gaatgtcgac gcttttcgcc agccagaccg
      541 gcttaccctg atccacggac tccgcgagcg cgagacggtc gaggtttttc tggatgagac
      601 ccgaaagttt cagcagcagc gccgagcgtt tttctttcgg catggtggac caggctggaa
      661 acgcggcctg tgccgcagcc tgagcggcgg ccacatcccg ttcgtcgctg tcgggaatca
      721 ggctgtaaac ttcgcccagc gccgggttga aattatcgag gtattgtccc gaagcaggcg
      781 ggacgaggac tccgttgatg tagttctgga ggtgaagcat gggggtaaag gtagtgaaga
      841 gtgaagagtg aacagtgaac agtgaatagt aaatagtaag tggcgaacag ttttttgtga
      901 atagcgaata gccaatagcg aatagttttt ggtgaatagt gagtgttttt aacctggttt
      961 tctttgaggc gggggaaata ggatttgata tttgtgattg gttatttgta attagaaatg
     1021 ccggttttat ttcgcctcaa tcaccaatca ccaatcacca atcaccaata cctacctact
     1081 aatcgctatc ttcgtatcaa caacacgttc gaacgtcaat gtcctacccc tttcaacata
     1141 ccgatcgacc agcttacgat gctgcctacc gtcgcagtgt acaggacccg gaaggtttct
     1201 gggccgatgt ggcggccaat ttttactggc gtaagcgatg ggaaaaaaca ctggagtgga
     1261 attttcggga cccggatgtc aaatggttct ccggcggcaa gttgaacatc acggaaaatt
     1321 gcctggaccg tcacctgggt acgctgggta acaagccggc gatcatctgg gaaccgaacg
     1381 atccggaaga gcatcaccgg gtactgacct accgcgactt gcacacgaaa gtcgtacagt
     1441 tcgccaatgt gttgaagaat aacggcgtca aaaaaggcga ccgggtctgc atctacatgg
     1501 gcatggtgcc cgagctcgcg atcgccgtac tggcctgcgc acgcatcggc gcgattcatt
     1561 cggtggtctt cggcggcttc agcgcgcaaa gcatcgccga tcgtctgacg gatgcacagg
     1621 ccgagttcat cgtcacctcc gacggtgcct ttcgtggcaa caaggagatc ggattgaaga
     1681 gcgtgatcga cgacgcgctg gtgcaatgcc ggtttgtcaa gcgggtgatc gtactcacgc
     1741 gtacgcggac tcccgtgtcc atgatcaaag gccgcgacgt ctggtgggaa gacgagatcc
     1801 gcaaagtgga aacgatgggc aatccggact gtccggcgga agaaatggac gcggaagatc
     1861 ctttgttcat cctctacact tccggatcca ccggaaagcc gaaaggcgtc gtgcattcga
     1921 gtgcgggcta tatggtgtac actacctatt cgttcgccaa tgtatttcag taccgcccgg
     1981 gcgagatctt cttctgtaca gccgacattg gttggatcac cggacacagc tatatcgtgt
     2041 acggtccgct cggcgccggc gccacctcgc tcatgttcga aggggttccc acctggccgg
     2101 acgctggaag gttttgggat atcgtcgaca agtaccacgt gaatatcctc tacaccgcgc
     2161 ctacagcgat ccgttcactc atgggatacg gactcgggcc gttgcagggt aagcacctca
     2221 acagcctgcg cgtactcggt acggttggcg aaccgatcaa cgaagaagcc tggaactggt
     2281 accacgagaa c
//
LOCUS       DAOTON010000465         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_36676_length_2289_cov_3.412713, whole genome shotgun sequence.
ACCESSION   DAOTON010000465 DAOTON010000000
VERSION     DAOTON010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36676_length_2289_cov_3.412713"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="P5292_09200"
     CDS             complement(<1..328)
                     /locus_tag="P5292_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39341.1"
                     /translation="MKTLSHLRHAAALLALATTGLTSCTKEDGFEPSQSAGIRDLRAD
                     AGTAEDRRRNDLPFDARDTKSIHDQGALDFTPALHGELDADDVVMIQIDKSALAGKAA
                     YKLMVSK"
     gene            complement(629..1207)
                     /locus_tag="P5292_09205"
     CDS             complement(629..1207)
                     /locus_tag="P5292_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRS39342.1"
                     /translation="MARASYYLINGITSYRVLAAPVLVWLIFTGEEDWFKWLLGISFF
                     TDLIDGYLARRFHVESVWGSRLDSVGDDLTVLAGFIGMYVFKGVFFHDHLFYFIFLGG
                     MFLVQTAIALIRFGKTTSFHTYLAKLSAILQGCFFILLFLLPTPVYPLFYTAVTFTFF
                     ELAEEIIITCYLKTWRTNVKGLYWVLRGNVNP"
     gene            1688..>2289
                     /locus_tag="P5292_09210"
     CDS             1688..>2289
                     /locus_tag="P5292_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39343.1"
                     /translation="MHPRLFLRASLATGCRSLMIFLAFLTFPLASYATHISGADITYR
                     WISGNTFELNLTLYRDCSGIAAPNNVSVSYSSASCGYNLSVTLNKVPGTGQEISQACS
                     TSVTTCAGGTYPGIQKWEYRGNVTLPAQCTDWRFGYSICCRNCAITTLVFAGGTCAGS
                     PALYVEATLNNVAAPTNSSPVFSNIPVSFLCIGQVFHYNHG"
BASE COUNT          523 a          569 c          631 g          566 t
ORIGIN      
        1 tcttggaaac catcagtttg tatgcggctt ttccggcaag cgccgactta tcgatctgga
       61 tcatgacaac atcgtcggca tccagttctc cgtgtaaggc aggagtgaaa tcgagcgctc
      121 cctggtcatg gatcgatttg gtgtcgcgtg catcaaaagg aaggtcattg cggcggcggt
      181 cttcggcggt tcctgcatca gcacgaaggt cgcggatgcc ggcgctttgg ctgggttcga
      241 aaccatcctc tttggtacaa ctggtaaggc cggtggtagc aagtgcaagc agtgcggcgg
      301 cgtggcggag gtgggacaag gttttcatgg cgtggagtat ggagggtttg tgttttcttg
      361 ttgtggttgc tcgtgtcgag cggtttgttg ttgtaatctg ttgtaatgat actgccttag
      421 gaagcggggt gtggatcccg cctgaacaag tggcgggttg accggataat tggcgggaaa
      481 tgacttggaa gaggacgaaa caggattcgg aacctttgat aaaatcatcc cgggtaaggc
      541 gtacgcggga tcggccatca tatcccgttc ccttaaccta ttccaaggtt ccggtgtgga
      601 gccccggatg ctgcaggatt gttgaaattc agggattgac gttcccccgc agcacccagt
      661 acaaaccttt tacattggtg cgccatgtct ttagatagca cgtgatgatg atctcttcag
      721 ccaattcaaa gaaagtgaag gtgacagcgg tataaaacaa cgggtagacc ggggtgggca
      781 acaaaaagag taaaatgaaa aaacaccctt gaagtatggc ggagagctta gcaagatagg
      841 tgtgaaaact ggtcgtcttg ccaaagcgga tcaatgcgat ggcggtttgt acgagaaaca
      901 taccgcccag gaagataaag tagaagagat ggtcgtgaaa gaatacccct ttgaacacgt
      961 acatcccgat gaatcctgcc aatacggtaa ggtcatcacc caccgaatcg agccggctgc
     1021 cccaaacgct ttcaacatga aagcggcgag caaggtagcc atcgatgagg tcggtgaaga
     1081 agctgatccc gagcagccat ttgaaccaat cctcctcacc ggtgaagatc agccacacca
     1141 gcaccggagc cgccagtacc cggtagctgg tgatgccgtt gataaggtaa taggaggctc
     1201 gagccatccg gataatgcta aaaagtaaag ttattgattc gtccggattg agaagaatgg
     1261 ttttccgttc tgctccaggg cgggtggaga tcgttcagct ttcgatttcc ggcgatcaga
     1321 cccgtttcct ggcatccgaa acactcttcg cgggtactgg ttgaaaaact gtaagactta
     1381 agccgattta tttctgaccg gagccgtccc gcttcgggcc aacgggtatc gggatgcctg
     1441 ggtttttgta agaaaatctc tctacagacg ctctacagga ggcatggatg atccgcaacc
     1501 cggctgtgtt tttcatttca acgcgtttct cctcctgcaa tatcgggtat ggactgaaag
     1561 taagccgagg gaaatgcgct aacgaatcat ggtgcgtttc gtctttattg taactatccg
     1621 aagtgggttc ggtggcttga acgccagttt cctgccgacg gtcggctaaa tttttatttg
     1681 aatggaaatg catcctcgac tttttctccg ggcatccctt gcgactggtt gtcgcagttt
     1741 gatgatcttc ctcgcctttc tcaccttccc attggcttcg tatgccacgc acatcagcgg
     1801 tgcggatatc acctaccgat ggatcagcgg taatacgttc gaactcaatc tcaccctgta
     1861 ccgcgattgt tctggcatcg cggccccgaa caacgtttcc gtcagttact cctccgccag
     1921 ttgcggctat aacctgagtg ttacgctgaa taaagttccg ggcaccgggc aggagatctc
     1981 acaagcctgt tccacctcgg tgaccacttg tgcgggtggc acctatccgg gtattcagaa
     2041 atgggaatac cgcggtaatg ttacgctgcc ggcgcaatgc accgactggc gattcggtta
     2101 ttccatctgc tgccggaatt gtgctatcac cacgctggta ttcgccggcg gaacctgcgc
     2161 gggatctccc gccctctatg tcgaggctac gttgaacaac gtcgcagcgc cgaccaactc
     2221 ttctccggta ttctccaaca tcccggtctc tttcctgtgt atcggacagg tgtttcatta
     2281 caaccacgg
//
LOCUS       DAOTON010000466         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_36685_length_2289_cov_2.411370, whole genome shotgun sequence.
ACCESSION   DAOTON010000466 DAOTON010000000
VERSION     DAOTON010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36685_length_2289_cov_2.411370"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="P5292_09215"
     CDS             <1..274
                     /locus_tag="P5292_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39344.1"
                     /translation="KPSCMRVGDRLYKNSFGLDRRIVNKCLTLASNCKRLRIPITTFM
                     VAEDPYLRSFIHEFSEANGGKAYYTSLKGLGQFVLEDFERNRVRRR"
     gene            303..1784
                     /locus_tag="P5292_09220"
     CDS             303..1784
                     /locus_tag="P5292_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRS39345.1"
                     /translation="MSLTKLTTLGALKASGYQSRSIKEELRANLLKKLQSGEKVFEGI
                     RAFDTTVIPDIERAILSRHNILLLGLRGQAKTRMARMMTRLLDEWIPVVKGSEINDDP
                     FHPISRYARDLIAGQGDDTVIEWMHRDERYTEKLATPDVSVADLVGDVDPIKAANLKL
                     PYSDERVIHFGLIPRSHRCIFVINELPDLQARIQVALFNILQEGDMQIRGFKLRLPLD
                     IEFVFTANPEDYTNRGSIVTPLKDRIESQILTHYPKDLESAREITRQEAHPSREQAEA
                     IRIPPLAEDLLEQIAFEARTSEYIDPKSGVSARLTISAKENLYSTVERRIILNKETTG
                     CVRIADFQGVIPSITGKIELVYEGEQEGTQIVAQNLVGKAVRTLFARYFPSPEKSKKK
                     KEPNPYQPVLDWFSSGHELDLLHDIPAKQYQKTLSGVPGLKEIVQQFHPQAKGDEQLL
                     LMEFLLHGLAEFSLLSRGNLQTGTRFKDLFSSVMNSGAGDEQS"
     gene            complement(1787..>2289)
                     /locus_tag="P5292_09225"
     CDS             complement(1787..>2289)
                     /locus_tag="P5292_09225"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRS39346.1"
                     /translation="AFALRLINDLFRLKIPVARLENYAARLGSDCPFFIQNKAVRATG
                     RGDVFESAKVDLKGYQLVIVKPRAHVSTAEAYSGITPKADRESLRVLVRKPVAQWREV
                     LVNDFEETIFIKHPSIRKIKEQLYKLGAVYASMSGSGSAVFGIFEQPTDVHMHFRSAT
                     VWQGRL"
BASE COUNT          540 a          622 c          653 g          474 t
ORIGIN      
        1 caagcccagc tgcatgcggg tcggtgaccg gttgtacaag aacagtttcg gccttgaccg
       61 caggatcgtc aacaagtgcc tgaccctggc gtccaattgc aagcggttac ggattccgat
      121 caccaccttc atggttgcgg aagatcctta cctgcgttcg tttattcacg aattctccga
      181 ggcgaacggg ggcaaggcct attatacatc gttgaaagga ctgggacaat tcgtcctgga
      241 agattttgaa cgcaaccggg tacgcaggcg ctgacccatg ttgttaaaaa aaattgactg
      301 atatgtcact gaccaaactg acgacactcg gcgcgctgaa agcgtccgga tatcaatccc
      361 gcagtatcaa ggaagagctg agggcgaatt tgttgaagaa actgcagtcg ggtgaaaaag
      421 tgttcgaagg cattcgcgca ttcgatacga ccgtcatacc ggatatcgaa cgagcgatcc
      481 tttcgcgcca caacatcctg ttactcggct tgcgtggcca ggccaagacc cggatggcgc
      541 gcatgatgac ccggctcctg gatgaatgga ttcccgtggt caaagggtcg gagatcaacg
      601 acgatccgtt tcatccgatt tcccgctacg cgcgtgacct gatcgccggt cagggtgacg
      661 ataccgtgat cgagtggatg caccgcgatg aacgttacac cgaaaaactg gcgacgcccg
      721 atgtgtcggt cgctgatctg gtgggtgatg tcgatccgat caaagccgcc aacctgaagc
      781 tgccttattc cgacgaacgg gtgatacact tcggacttat tccccggtca catcgttgca
      841 tcttcgtcat caacgaacta ccggatctgc aagcgcgcat ccaggtggcc ttgttcaaca
      901 tcctgcagga gggcgatatg cagatccgtg gattcaagtt gcgcctgccg ctcgatatcg
      961 agttcgtctt tacggccaat cccgaggact atacgaaccg tggttcgatc gtcaccccgc
     1021 tgaaggaccg catcgaaagc cagatcctga cgcactatcc gaaagacctg gagagcgcgc
     1081 gggagatcac ccggcaagag gcgcatccaa gccgggagca ggcggaggcc atccgaatcc
     1141 cgccactcgc ggaagacctg ctggagcaga tcgccttcga agcccgtacc agcgagtaca
     1201 ttgatcccaa gagcggggtg tcggcgcgcc tgacgatctc ggccaaggag aatctttaca
     1261 gtacggtgga acgcaggatc atcctgaata aggaaacgac cggctgcgtg cgcatcgcgg
     1321 atttccaggg tgtcattcct tccatcaccg gaaagatcga actcgtgtac gaaggcgagc
     1381 aggagggaac gcagatcgtc gcacagaacc tggtaggaaa ggcggttcgc accttgttcg
     1441 cccggtattt cccgagtccg gaaaagagta agaagaagaa agagccgaat ccctatcagc
     1501 ccgtgttgga ctggttcagc agcggacatg aattggacct cttgcacgac attccggcta
     1561 agcagtacca aaagacctta agcggtgtgc cgggactgaa ggagatcgtt cagcaatttc
     1621 acccgcaggc caaaggtgat gagcaactgc ttctcatgga attcctgttg cacggactcg
     1681 cggaattctc gctgctaagt cggggtaatc tgcagacggg gacacggttc aaggacctgt
     1741 tcagttcggt catgaacagc ggcgccggtg atgagcaaag ctgagctcag agtcggcctt
     1801 gccagaccgt agcgctccgg aagtgcatgt gaacgtcggt cggctgttca aaaattccga
     1861 agaccgcgga accacttcca ctcatggagg cataaacagc gccgagcttg tagagttgct
     1921 ccttgatctt gcggatggaa ggatgtttga tgaagatcgt ttcttcgaaa tcattcacga
     1981 gtacctcccg ccactgcgcc accggtttcc tgaccagtac ccgaagtgat tcccggtccg
     2041 ccttgggcgt aatgccgcta taagcctcgg ctgtgctcac atgtgcgcgc ggttttacga
     2101 tcaccagttg atagcctttg aggtccactt tcgccgattc aaaaacatcg cctcgaccgg
     2161 tggcacgaac cgctttgttc tggatgaaga agggacaatc gcttccgagc cgggccgcat
     2221 agttttcgag gcgggcaacc gggatcttca ggcggaacag gtcgttgatc aaacgtaagg
     2281 cgaaggctg
//
LOCUS       DAOTON010000467         2288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_36705_length_2288_cov_2.780116, whole genome shotgun sequence.
ACCESSION   DAOTON010000467 DAOTON010000000
VERSION     DAOTON010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36705_length_2288_cov_2.780116"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(561..>2288)
                     /locus_tag="P5292_09230"
     CDS             complement(561..>2288)
                     /locus_tag="P5292_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRS39347.1"
                     /translation="AANRTKLDKARYQLARAEGLEALAKAKNPKDPGLPNLRRQIAAY
                     DGYHSVSLTGGEPLVQAEFLASFLPLMRQDRRKIYLETNGTLWREFSLVRDDVDIVAM
                     DIKLPSSGGQKPCWLKVVEFLNRQNDFNWEWADLRTLWSAAQVTGEVAVGYKPLSVND
                     VDPIIHKINIHSAEWKAVHDALIERVLQQLNSTGTKTYTRDEIVAEDDPVLPLMILRV
                     TDKTALTLLRNIENVRYLEPYGYWPQNMTMRSMSSSGCSSSTEPLNSADWTTITPGCR
                     LPWNYNNMNIPTAWNTAEGQGIQIGVIDAGLSSSQALMGAQFNDGMSNVGRTVTVDYT
                     RGNSAYTSCTHGTSMASTAAGPRNAQNATTGVAYKSSLHFIRSCDDVVLDASAELSGT
                     KNALIRMGDMPGIRVISMSIGTPFGSGVLKDGVNYANGKGKLIFAAAGTSFSWTSWWG
                     VIYPAAYSACVAVTGVKESGSTCATCHDGSQVRFTVPMERTANSNRNSLALMPNGTTP
                     AYIGGSSVATSTAAGVAALVWSAKPTLTSAQVLDIMTRTAQFYPSRNSTKGFGNINAA
                     AAVTLATTY"
BASE COUNT          442 a          596 c          753 g          497 t
ORIGIN      
        1 gggggggagg tgattcatat gtccacaggt tgtcatgaaa catcgatggt gattcaatgt
       61 cccatgcgtt taatctactg ccggaagttt tgaaccaccg ccccggaccc ctttaggggt
      121 cccggtatcc tagccaacgg cgttttttta tttttcttct gacgaagctc gtcgcccgta
      181 aatagccccg atggcagcga gctcccgcga gcggacagcg ggaggagacg gttcaacgaa
      241 gtgatcaacg tgtccgcttc gcggagggac gagggacgag atgcgaggga cgaaggttag
      301 gtgcgaggtg cgagcagcga cggatgaaaa gaacatacct gtagcgagtt aatttgagac
      361 attcacggga aattttcata aggcctgcaa cgggaatttt ccggttaaaa aaaacgggag
      421 acactacatg gagcgtctcc cgtgggtgga agaggtgatt gtaattggta attggtaatt
      481 agtaattaga actggaaact tatcactaat cacaaattac tagttactaa ttactagtta
      541 ctagttacca gttttaaccg tcaatacgtc gtggcgagcg tcaccgccgc cgctgcattg
      601 atattgccga atcctttcgt cgagttgcgc gacgggtaga attgcgcggt gcgggtcatg
      661 atgtcgagga cttgggcgga ggtgagggtc ggtttcgcgg accacaccag cgcagcgaca
      721 cccgctgccg tagaggtcgc taccgagctg cctccgatgt aggccggtgt cgtaccgttg
      781 ggcatcagcg caagcgagtt gcggttgctg ttggccgtgc gttccatggg cacggtgaag
      841 cgtacctggc taccgtcgtg gcaggttgcg caggtggaac cgctttcttt cacgccggtg
      901 accgctacgc aggcggagta ggctgccggg tagatcacgc cccaccagct cgtccaggag
      961 aacgaagtgc cggccgcggc gaagatgagt ttgccttttc cgttggcata gttcacgccg
     1021 tctttgagca caccgctgcc gaagggcgta ccgatcgaca tgctgatgac gcggatgcca
     1081 ggcatatcgc ccatgcggat gagggcgttc ttcgtgccgg aaagttcggc ggaagcatcg
     1141 agcacgacgt cgtcgcagga gcgaatgaag tggagactgg atttgtaagc gacaccggtc
     1201 gtcgcgtttt gcgcgttgcg cggacctgcg gcggtggagg ccatcgaggt gccgtgggtg
     1261 cagcttgtgt aggcgctgtt gccgcgggtg taatcgacgg tgacggtacg gccgacgttg
     1321 ctcatgccgt cgttgaactg cgcgcccatc agggcctgcg aggagctgag gccggcatcg
     1381 atcacaccga tctggatgcc ttgtccttcg gcggtgttcc aggcggtggg gatgttcatg
     1441 ttgttgtaat tccaggggag acgacaaccg ggcgtgatcg tggtccagtc ggcgctgttg
     1501 agcggctcgg tggagctgct gcagccggag gaggacatgg agcgcatcgt catgttctga
     1561 ggccagtagc cgtacggttc gaggtagcgg acgttctcga tgttccgcag cagggtgagc
     1621 gcggttttgt cggtgacgcg caggatcatc aagggcagaa ccggatcatc ctcggcgacg
     1681 atctcgtcgc gggtgtaggt cttcgtgccg gtgctgttga gttgctgcag cacgcgttcg
     1741 atgagcgcgt cgtgcaccgc tttccattcg gcggagtgga tgttgatctt gtggatgatc
     1801 gggtccacgt cgtttaccga gagcggtttg taacccacgg ccacttcgcc ggtcacctgg
     1861 gcggcgctcc agagggtgcg gaggtcggcc cattcccagt tgaaatcgtt ctgccggttg
     1921 aggaactcca cgacctttag ccaacagggc ttctggccgc cgctggaagg cagcttgata
     1981 tccatagcca caatgtccac atcgtcgcgc accagcgaaa actcccgcca cagcgtgccg
     2041 ttcgtctcca gataaatctt ccgccggtcc tgacgcatca aaggcagaaa cgaagcaagg
     2101 aattccgcct gaaccagagg ttcgccgccg gtcagagaca cggaatgata accgtcgtaa
     2161 gcggcgatct ggcggcgcag attcggcagc ccggggtcct tgggattctt ggccttcgcc
     2221 agcgcctcga ggccttcggc ccgggcgagt tgatagcggg ccttgtcgag cttggtgcgg
     2281 ttcgcggc
//
LOCUS       DAOTON010000468         2282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_36836_length_2282_cov_3.585990, whole genome shotgun sequence.
ACCESSION   DAOTON010000468 DAOTON010000000
VERSION     DAOTON010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36836_length_2282_cov_3.585990"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="P5292_09235"
     CDS             <1..334
                     /locus_tag="P5292_09235"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRS39348.1"
                     /translation="NLCLHLIGNLRHFIGHLLGGGDYARDREYEFNAKDLTRAQLLDL
                     INTCQHEFDTGLANIETNRLGEIAPAKAAGHEVTVRQLMFHLLAHFAYHLGQINYHRR
                     FGMNGKIA"
     gene            388..1131
                     /locus_tag="P5292_09240"
     CDS             388..1131
                     /locus_tag="P5292_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRS39349.1"
                     /translation="MNDESLVLIPTYNEKENIERMVRKVFSLPHPFHLLIIDDGSPDG
                     TADIVRKLQSEFQGRLFLEERKGKLGLGTAYIHGFKWALARSYQYIFEMDCDFSHNPD
                     DLIRLYQACAVEGADVAIGSRYIRGANVVNWPLGRVIMSYYASVYVRIITGIRIMDTT
                     AGFICYRRRVLETIELDRIRFIGYAFQIEMKFTAWKHGFRIVEVPIIFTDRTEGTSKM
                     SKGIFKEAILGVMQMRWKSFFRKYQKVKN"
     gene            1138..>2282
                     /locus_tag="P5292_09245"
     CDS             1138..>2282
                     /locus_tag="P5292_09245"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRS39350.1"
                     /translation="MPSYLIRNARIVNEGRITEGDVRIRNGRIDAIGTLTASSQDRVI
                     DARGHYLLPGAIDDQVHFREPGLTHKGDLHTESVAAVAGGITSYMEMPNTQPPALTRD
                     LLEDKYRRAHEVSLANYSFYMGTSNDNLEEVLRTDNRTVCGVKIFMGSSTGNLLVDEP
                     NTLEAIFARSESLIATHCEDEATIRANLEAARIRYGEQVPIREHADIRSETACYLSSS
                     FATGLAKKHGTRLHVLHISTAKELELFSNELPRTQKKITAEACLHHLWFSETDYDRLG
                     TRIKWNPSIKRAEDRDAILKAVLDGRIDVVATDHAPHTVEEKMRDYFQAPSGGPLVQH
                     LLPALFEFVKRGTMRVEDVVDRTSHAVADLFRIVDRGYIREGYWADLV"
BASE COUNT          543 a          661 c          591 g          487 t
ORIGIN      
        1 aaatctatgc ctgcacctga tcggtaatct gcgccatttc atcggtcacc tgctgggtgg
       61 aggagactac gcccgtgacc gggaatacga gttcaatgcg aaagacctga cgcgcgcgca
      121 actgctcgac ctcatcaaca cctgtcagca tgaatttgat accggcctgg ctaacattga
      181 gaccaaccgg ctcggggaga tcgcccctgc aaaggcggcc ggtcatgagg tgaccgttcg
      241 ccagttgatg ttccatctgc tggcgcactt tgcctatcat ctcggccaga tcaactacca
      301 ccgtcgcttc ggaatgaacg gcaaaatcgc ctgaaaatcg ggtttacgcc gattccgtat
      361 cttgcaaact gtctactccc ccggtttgtg aacgacgaat cactggtatt aattcctact
      421 tacaacgaga aggagaacat cgaacggatg gtccgcaagg tcttttccct tccccacccg
      481 ttccacctgc tcatcatcga tgacggctct ccggatggaa cagccgacat cgtcagaaag
      541 ctacagtccg aattccaggg tcgtctgttt ttggaagagc ggaaaggaaa gctcggtctg
      601 ggcacagcct acattcatgg attcaaatgg gcattggcaa ggagttatca gtacatcttc
      661 gagatggatt gcgatttctc acacaatccc gacgacctca tacgactgta tcaggcctgt
      721 gctgtggaag gggccgacgt cgcgatcggc tcacgctata tccggggtgc caacgtcgtg
      781 aactggccgc tcggacgggt catcatgtcc tactacgctt ccgtttacgt gcggatcatt
      841 accggcatcc ggatcatgga tactacggcc ggatttatct gctatcgtcg acgcgtgctg
      901 gaaacgattg aactcgaccg tatccgtttc atcggatacg cgtttcagat tgaaatgaaa
      961 ttcaccgcat ggaagcacgg cttccgcatt gttgaagttc ccatcatttt caccgaccgt
     1021 accgaaggca cgagcaaaat gagcaagggc atcttcaaag aagccatact cggcgtgatg
     1081 cagatgcgct ggaagagttt tttccgtaag taccaaaaag taaagaactg atccatgttg
     1141 ccctcgtacc tcatccgtaa tgcacgcatc gtgaacgaag gtcgcatcac ggaaggcgat
     1201 gtcaggatcc gaaatggcag gatcgatgcg atcggcaccc taacggcttc cagtcaggac
     1261 cgggtgatcg atgcacgcgg ccattacctc cttcccggcg ccatcgacga ccaggtacat
     1321 tttcgtgaac ccggactcac gcacaaaggc gatcttcata ccgagtcagt ggccgccgtt
     1381 gccggtggca ttacctcgta tatggaaatg cccaacacgc aacctccggc attgacccgc
     1441 gacctgttgg aggacaaata ccggcgtgcg cacgaggtgt ccctggccaa ttactcgttc
     1501 tacatgggca cttcgaacga caacctcgag gaagttctgc gaacggacaa tcgcacggtc
     1561 tgcggagtga agatctttat gggctcctcc accggcaacc tgctggtgga cgaacccaat
     1621 acactcgagg ccatcttcgc acgttcggaa tctctcattg cgacgcattg tgaagacgaa
     1681 gcgaccatcc gtgccaacct ggaagctgcc cgaattcgat atggagaaca ggttccgatc
     1741 cgggaacatg cagacatccg cagcgaaacc gcctgctatt tgtccagttc cttcgctacc
     1801 gggctcgcga aaaaacacgg cacccgcttg cacgtgctgc acatcagcac ggccaaggag
     1861 ttggaactct tcagcaacga gctgccgcgc acgcagaaaa agatcacggc tgaagcctgt
     1921 cttcatcacc tctggttttc cgaaaccgat tatgaccgcc tcggaactcg catcaagtgg
     1981 aacccctcca tcaagcgtgc tgaagaccgc gacgcgattc taaaagccgt actcgacgga
     2041 cggatcgacg tggtcgccac ggatcacgcc ccgcatactg tggaagaaaa aatgcgcgac
     2101 tactttcagg ctccctccgg cggtccattg gtacaacacc tgctgccggc acttttcgaa
     2161 ttcgtgaaac gcggaacgat gagggtagaa gacgtcgtgg atcgcaccag tcatgccgta
     2221 gccgacctgt tccgcatcgt ggaccgtggg tatatccgcg aaggttattg ggcagacctc
     2281 gt
//
LOCUS       DAOTON010000469         2280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_36885_length_2280_cov_2.594157, whole genome shotgun sequence.
ACCESSION   DAOTON010000469 DAOTON010000000
VERSION     DAOTON010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36885_length_2280_cov_2.594157"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="P5292_09250"
     CDS             <1..1216
                     /locus_tag="P5292_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS39351.1"
                     /translation="NAAIRIQNQRCSPVFTFIDSSYGIGGTSNYWQWTFGDGQVAPTE
                     AATHQYAAPGQYNVTLIASTDSGCTDTTSRLIHVLQLPEPSFSLELDTCSFLLRTQNT
                     SAYASTYQWNFGNGMDATAGDTSFVFADDGLYTITLEAVTDSGCARSTSAALFIPPLP
                     VADFTWEVAECDSTVRFDNRSADALTFDWEFGDGATASGENVSHTYEKAGSIPVTLIA
                     RSRYGCLDTLVQDISFTSYKGAAFDWQLDSCSGELQFFNVTRNAVTYRWDFGDGTISL
                     QETPVHKYQANGSYRVLLTVNGESACIDTVSDLTVYESPLGERLFVPNAFTPNGDGKN
                     ERFVISVYRPCDHYELFVFDRWGQEIYHAEDAVTASWDGTIAGRPAETGLYVYLLRSA
                     SGNRTGTITLLR"
     gene            complement(1217..>2280)
                     /locus_tag="P5292_09255"
     CDS             complement(1217..>2280)
                     /locus_tag="P5292_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRS39352.1"
                     /translation="IGNTGYLCTGYDGTWTVTDCWSYNFQNDTWTQIADLPGVSRFAA
                     AAFSVNQQGFVGFGWANPSGGQLADFWRYNPVTNSWTALANFPGASRYTVVTLVLNGK
                     AYCGTGYLPYTNDWWVYDPVTDTWTQKANFPGSARQSASGFALNGIGYLGLGYDGGCN
                     VDFFAYDPNVNNWNQVADFPGPARYGAAAFTQNGLGYVGTGGDGVDFFSDFFSYDPVS
                     DQWSPIDSLPGPGCRHPATFSLSNKAYVATGIVQGADGSDAVYEYGPFDPSGIEDIKS
                     PVASWQIVQNANLQLSLSIPATSQLPSQIELFSTEGKLIYSTKDCSSFNFAPHPQTIY
                     LYRLTHPDGYEKAGKLVTR"
BASE COUNT          530 a          607 c          599 g          544 t
ORIGIN      
        1 caatgccgca atccggatac aaaaccagcg atgctcaccg gtctttacgt tcattgactc
       61 gtcctacggc attggtggaa cttcgaatta ctggcaatgg acattcggcg acgggcaggt
      121 cgcgcctacg gaagcggcta cccatcagta tgccgctccc ggtcaatata acgtgacgct
      181 cattgcctcc accgactcgg gctgtaccga tactacctcg cgactgattc atgtcttgca
      241 acttccggag ccttcgttca gcctggagct ggatacctgc agcttcctgt tgcgaacaca
      301 gaatacttca gcgtatgctt ccacctatca gtggaatttc ggaaacggca tggacgcgac
      361 cgccggcgac accagtttcg tttttgctga cgatggtctc tataccatca cgctcgaagc
      421 ggtaaccgat tccggttgtg cccgcagcac ttccgccgcg ctcttcattc cgccattgcc
      481 ggtagccgac ttcacgtggg aagtcgcgga atgtgattcc accgtccggt tcgacaaccg
      541 ttccgcggat gccctgacat ttgactggga gttcggcgat ggcgcgacag catccgggga
      601 aaatgtcagc catacctatg aaaaggccgg cagcattccg gttaccctga tcgcacggtc
      661 gcgttatgga tgtctggata ccctggtgca ggacatttcc ttcaccagct acaaaggcgc
      721 ggccttcgac tggcagcttg atagttgttc gggtgaattg cagttcttca atgtcacgcg
      781 caacgcggtg acgtatcgtt gggatttcgg cgacggtacg atcagcttac aggagacacc
      841 cgtgcacaaa taccaggcga acggttcgta ccgggtgttg ctgacggtaa atggagaatc
      901 agcctgtatc gatacggttt ccgacctgac ggtttatgag tcgccgctcg gggagcggtt
      961 gtttgtgccg aacgctttta caccgaatgg cgatggcaag aacgaacgat ttgttatctc
     1021 cgtttatcgc ccctgcgacc actatgaatt gttcgtcttt gaccgctggg gacaagagat
     1081 ctatcacgcc gaggacgccg tcacagcctc ctgggatggc accatagcag gaaggccggc
     1141 cgaaacaggt ctgtatgtct acctgctgcg gtcggcctcc ggaaatcgta ccggtaccat
     1201 tacactgctt cgctgatcaa cgcgtcacca gcttcccggc cttctcgtat ccgtctggat
     1261 gggtgaggcg gtagaggtag atggtttgcg ggtggggggc gaagttgaag gaactgcaat
     1321 ctttggtact atagatcagt ttcccttccg tactgaataa ttctatttgt gatggtagtt
     1381 ggctagttgc aggtatggag agtgataatt gaaggttagc attctgaaca atctgccagg
     1441 aagccaccgg agacttgatg tcctcaattc cagatggatc aaatggtcca tactcatata
     1501 cggcatcgga gccatctgca ccttgtacga ttccggtggc tacataggct ttgttgctca
     1561 gcgagaaggt tgccggatgt ctgcagcctg ggcccggtag tgaatcgatc ggggaccatt
     1621 gatcagacac gggatcatag gagaagaagt cactgaaaaa gtcaacacca tctccacctg
     1681 tgcccacata accaagtcca ttctgcgtaa atgcggcagc tccatagcgc gcaggaccag
     1741 gaaaatcagc gacttgattc cagttattta cgtttggatc ataagcgaaa aagtcgacgt
     1801 tgcaaccacc gtcatatcca agtccaaggt aacctattcc attgagcgcg aaacctgaag
     1861 cactctgtct ggctgaaccc ggaaaatttg ctttttgtgt ccaggtatcg gtgacaggat
     1921 catacaccca ccaatcattt gtatagggaa gataccccgt cccgcaatac gccttaccgt
     1981 tcaacacaag agtaacaact gtataacgtg aagcaccggg aaaatttgcc aatgcggtcc
     2041 atgaattggt aacaggattg tagcgccaga aatcggccaa ttgtccgccg ctcgggttgg
     2101 cccaaccgaa tccgacaaat ccttgttgat taacactgaa tgctgccgca gcaaatcggg
     2161 atacacccgg cagatcggct atttgtgtcc aggtgtcgtt ttgaaaatta tacgaccagc
     2221 aatctgtgac agtccatgtg ccgtcgtatc cggtgcacag ataccccgtg ttaccgatgc
//
LOCUS       DAOTON010000470         2272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37051_length_2272_cov_3.465043, whole genome shotgun sequence.
ACCESSION   DAOTON010000470 DAOTON010000000
VERSION     DAOTON010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37051_length_2272_cov_3.465043"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /gene="gpmI"
                     /locus_tag="P5292_09260"
     CDS             <1..1460
                     /gene="gpmI"
                     /locus_tag="P5292_09260"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRS39353.1"
                     /translation="AIFKARTPFIDSLYANYPNAELLTSGEDVGLPAGQMGNSEVGHL
                     NIGAGRVVYQDLVRINLAIRDGSIFREKTLVEAFAFAKANNKPVHFLGLVSDGGVHSH
                     LEHLKALCDAAKKDEVQDVYIHAFTDGRDTDPKGGLGYLRELQEHLKHSSGKIASIVG
                     RYYAMDRDKRWERVKEAYDLLVSGHGTPATDPLKAIESSYTAGITDEFIKPIVITRPD
                     GQPTAIIREGDVVICFNFRTDRCREITQALTQQDFHEQNMHRMDLHYVTMTNYDDTFR
                     NVHVIYDKDNLAMTLGEVVANAGLKQIRIAETEKYPHVTFFFSGGREAPFEGEQRILV
                     PSPKVATYDLQPEMSAEEVTRKLCAELEKGEASFVCLNFANCDMVGHTGVFDAVVTAV
                     EKVDDCVRRVVETGQRNGYSFLITADHGNADYMINEDGSPNTAHTTNPVPVFLLDSHH
                     RTLQNGRLADLAPTVLHLMGLEIPKEMTGKVLCGT"
     gene            complement(1593..>2272)
                     /locus_tag="P5292_09265"
     CDS             complement(1593..>2272)
                     /locus_tag="P5292_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS39354.1"
                     /translation="ESTPGELIIPNADLKPERTTSGELGITLSLGKNVRWENTGFYTA
                     FADAIVTDVTKFNGQDSVLYDGTMSRVYSNQNKQEAYIYGFSSAIEIEAARDWHIVGS
                     VNYTYGRVKTDTVEIPLDHIPPLYGRLAVRYQHEKFEAEASTVFNGKKAIDDYNPFGE
                     DNQQYATADGMPSWYTVNLRTQYRLKSFLTLQLGLDNILDQNYRAFASGIQAPGRNLI
                     AAVRVNW"
BASE COUNT          540 a          624 c          589 g          519 t
ORIGIN      
        1 acgcgatctt caaggcgcgt acccccttca tcgattccct ttacgcgaat tatccgaatg
       61 ccgaattgct cacgtccggc gaagacgttg gtttaccggc cggacaaatg ggcaacagcg
      121 aagtcggtca cctgaacatc ggtgccggac gcgtcgtgta ccaggatctt gtccgcatca
      181 acctcgcgat ccgtgacgga tctatcttcc gggaaaagac cctggtggaa gcattcgctt
      241 tcgcgaaggc gaacaacaag ccggttcatt tcctgggtct ggtttccgac ggcggcgtgc
      301 attcgcacct cgagcacctc aaggcccttt gtgacgcggc gaaaaaagac gaggtgcagg
      361 atgtttacat ccatgccttc accgatggac gcgatacgga tccgaagggt ggcctgggtt
      421 atctgcgcga gttgcaggaa cacttgaaac attcatctgg taaaatcgcc agcattgtcg
      481 gccggtatta cgcgatggat cgcgacaaac gttgggaacg cgtcaaggaa gcctatgatc
      541 tgctggtatc gggccatggc acgcccgcaa ccgatccgct gaaagcaatt gaatcttcgt
      601 atactgccgg cataaccgat gaattcatca aaccgatcgt tattacacgt cccgacggac
      661 aaccgacggc gatcatacgg gaaggcgatg tcgttatctg tttcaatttc cgaaccgacc
      721 gctgtcggga gatcacgcag gcgctgacac agcaggattt tcacgagcag aacatgcacc
      781 gcatggatct gcactacgtg accatgacga attacgacga tacgttccgg aacgttcacg
      841 tcatttacga taaggataac cttgccatga cgcttggaga agtggtggcc aatgcgggtt
      901 taaaacagat ccggatcgcg gaaacggaaa agtacccgca cgttacgttc ttcttttccg
      961 gcggtcgtga agcacccttc gaaggcgagc aacgcatcct ggtcccttct ccgaaagtcg
     1021 ccacctacga ccttcaaccc gagatgagtg ccgaagaggt tacgcgtaaa ctctgcgccg
     1081 aactggagaa aggcgaagcc tcgtttgtct gcctgaattt cgccaactgc gacatggtcg
     1141 gacatactgg tgtgtttgac gcggttgtca cggctgtcga aaaagtggac gactgtgttc
     1201 gtcgcgtagt agagaccggt cagcgcaacg gctactcatt tctaatcacc gccgatcacg
     1261 gcaatgccga ctacatgatc aacgaggatg gatcaccgaa caccgctcat acgaccaatc
     1321 ccgttccggt ttttctgctc gactcccatc accgcacact ccaaaacggc cgccttgccg
     1381 accttgctcc caccgtgttg cacctgatgg gtttggagat tccgaaggag atgacgggaa
     1441 aggtgttgtg cggaacctga tgcagaggca gaaaaaatcc ggcatcgtga tgcaggtaat
     1501 taacaactgg aaaactggag gaagaaactt ctccaatcac caatcaccag tttccaatta
     1561 ctagttacta gttactagtt tccagtctcc acctaccaat tcaccctcac cgccgcgatc
     1621 aaattccttc ccggcgcctg gataccgctg gcgaatgccc ggtagttttg atccaggatg
     1681 ttatcgagcc ccagctgcag ggtcaggaat gatttcaatc tgtactgcgt gcgcaggttg
     1741 acggtatacc aggaaggcat cccatcggcg gtggcgtact gctggttatc ttctccgaaa
     1801 ggattgtagt cgtcgatagc tttcttgccg ttgaataccg tggacgcttc agcttcgaat
     1861 ttctcgtgct gataacgcac cgccaaacga ccgtagagcg gagggatatg atccagcgga
     1921 atttccactg tatcggtttt cacccgtcca taggtgtaat tgaccgatcc cacaatatgc
     1981 cagtcgcggg cagcttcaat ctcgatggca gaactgaagc catagatata ggcttcctgc
     2041 ttattctgat tgctgtaaac acggctcatg gtcccgtcgt agagcacgct atcctgcccg
     2101 ttgaatttcg taacatccgt tacgatggca tcggcaaagg ccgtatagaa accggtattt
     2161 tcccaccgta cattcttacc cagggaaagc gtgattccaa gttccccgga ggtcgtccgt
     2221 tccggcttga gatcggcatt gggaatgatc aactcaccgg gcgttgattc ga
//
LOCUS       DAOTON010000471         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37091_length_2270_cov_3.103386, whole genome shotgun sequence.
ACCESSION   DAOTON010000471 DAOTON010000000
VERSION     DAOTON010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37091_length_2270_cov_3.103386"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="P5292_09270"
     CDS             <1..700
                     /locus_tag="P5292_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRS39355.1"
                     /translation="TGAGKLNTSQVVISNFPAFNKLADALKMPSWKKLDVPALNPSFR
                     FVNGRVYVDPFDVTMNGNKATIAGSNGFDQTIDYTMAVQIPRSSFGGNANAALEGLVS
                     KANASGANFSLGETVPLNVTIGGTVLEPKVGTDLNKKGAAVMDDLKAKAKAEFDKKKA
                     EAEAKARAEADKLKAEAEAKLNAEKEKAKAEADRLKKEAEAKAKAAADSAKKAAEKKG
                     KEMIDEFNPFKKKK"
     gene            697..>2270
                     /locus_tag="P5292_09275"
     CDS             697..>2270
                     /locus_tag="P5292_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS39356.1"
                     /translation="MIKAVCPIGRFLIAGTLLLNSCLAVAQSCPKSENKKALRFQEEA
                     ESAYKSRKYEDALDASGKAIDADPEYADAYLLQGYAALKKRSFGLAEESLQKAIELCE
                     SVDPEAYYQLGWLYYDLKKWKESERYLSKFTGHDRINEEHGAKAELMLARAKLYAHPV
                     PFDPQPVKDLSTPDPEYLPYISPDQDLAFFTRRFELKDKNMLVPQSVEKFMLSKRTGL
                     GVYERGAPMPLPFNQASSNNEGGATITIDNKHLFFTVNTKGNFDICTSDRENGQWGEI
                     RNLGPNVNDPKQWDSQPTIAPDGKTLYFASARDAATGIDLYVTTRSDDGTWNKARKLP
                     PPVNTNGNDKSPFFHSDARTLYFSSDSLPGLGGYDIYFSRQDEKGNWSKPVNLGYPIN
                     TEADEVGFFVSTDGKKGFFSSNKLNNGAGGFDIYSFDLYEGARPGKVYFQKGDLNGPE
                     DADPLPATIEIKDAVTRKLTRIDVDSVTGEYAFVVNFDHDLLVSVKKEGYAFESQYVS
                     RKDTARPLVVTSDLQL"
BASE COUNT          614 a          547 c          648 g          461 t
ORIGIN      
        1 caccggtgca ggaaagctga acaccagcca ggtggtgatc tccaatttcc ctgctttcaa
       61 caaactggcg gatgcgctga agatgccctc ctggaaaaag ctggatgtac ccgcgttgaa
      121 tccttccttc cgttttgtca atggccgcgt ctatgtggat ccgttcgatg tgaccatgaa
      181 cgggaacaag gcgaccattg caggatcgaa cggattcgac cagaccatcg actataccat
      241 ggctgttcag attccgcgga gttcgtttgg cggcaacgcc aacgcagcac tcgaaggtct
      301 cgtttccaag gcgaatgctt ccggagccaa tttcagtttg ggagagacgg tcccgttgaa
      361 tgtgaccatt ggcggtacgg tgctggagcc caaggtcgga acggacctca ataaaaaagg
      421 cgcagcggtt atggatgacc tgaaggccaa ggccaaagcg gagtttgata agaagaaggc
      481 tgaagccgaa gcgaaagccc gggccgaggc cgacaagctc aaggcggaag cggaagcaaa
      541 gctgaacgcc gagaaggaaa aggccaaggc ggaagcggat cgtttgaaga aagaagcgga
      601 agcgaaagcg aaagcagctg ccgacagcgc gaagaaagcc gcggagaaga aagggaagga
      661 gatgatcgac gaattcaatc cgttcaagaa gaagaagtga taaaagccgt ttgtcctatt
      721 ggcaggttcc tgatcgcggg aacattgctg ttgaactcct gcctggcggt tgcgcaatca
      781 tgcccgaagt cggagaataa aaaagccctc cgcttccagg aggaggcgga gtcggcttac
      841 aagtcgcgca agtacgaaga tgcgttggat gccagtggaa aagcgattga cgcggatccg
      901 gaatatgcag acgcttattt gctgcaaggt tatgccgcat tgaagaaacg ctcattcgga
      961 ttggcggaag agagtttgca aaaggcgatc gaactctgcg agtcggtcga tccggaagcc
     1021 tattatcagc tgggttggtt gtattatgac ctcaagaaat ggaaggagtc ggagcgctac
     1081 ctgtcaaaat tcacaggtca tgaccggatc aacgaagaac atggtgccaa ggccgaattg
     1141 atgctggcaa gggcgaagct ttatgcccat ccggtaccct ttgacccgca accggtgaaa
     1201 gacctgagca cacccgaccc cgagtatctg ccgtacatat cacccgacca ggacctggcg
     1261 tttttcaccc gccgctttga actgaaggac aagaacatgc tggtgccgca aagcgtcgag
     1321 aaattcatgc tttcgaaacg caccggtctg ggcgtttatg agcgaggtgc cccgatgcca
     1381 ttacccttca atcaggcaag ctcgaacaac gaaggcgggg caacgatcac gattgacaat
     1441 aagcacttgt tctttacggt caatacaaag ggtaatttcg acatctgtac ttcggatcgc
     1501 gaaaatgggc agtggggaga gatccggaat ctcggcccga atgtcaacga cccgaagcaa
     1561 tgggattcgc agccgaccat tgcgccggac gggaagacgt tgtacttcgc ttcagcaagg
     1621 gatgcagcca cgggaattga tctctatgtg acaacgcggt cggatgatgg aacatggaac
     1681 aaggcgcgta agctcccgcc tcccgtcaac acgaacggga atgataaatc gcctttcttt
     1741 cacagcgatg cccggacctt gtatttctct tccgacagtc tgcctggatt gggcggatac
     1801 gatatttact tttcccgaca ggatgagaag ggtaactgga gcaaaccggt gaatctgggc
     1861 tatccgatca atacggaagc cgatgaagtg ggattctttg tcagcaccga tgggaaaaaa
     1921 ggatttttca gctccaacaa attgaataac ggagccggcg gtttcgatat ctattccttc
     1981 gatttgtatg aaggcgcccg accggggaag gtgtattttc aaaagggcga tctgaacgga
     2041 ccggaggatg ctgatccatt acctgctacg atcgagatca aggatgcggt gacaagaaaa
     2101 ctcaccagga tcgatgtcga ttccgtgacc ggtgaatatg ccttcgtcgt aaacttcgat
     2161 cacgacctcc tggtatccgt taagaaagaa ggctatgcgt tcgaatccca gtatgtatca
     2221 cggaaagaca cggcccgacc attggtagtt accagcgatc ttcaactgaa
//
LOCUS       DAOTON010000472         2269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37117_length_2269_cov_3.927281, whole genome shotgun sequence.
ACCESSION   DAOTON010000472 DAOTON010000000
VERSION     DAOTON010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37117_length_2269_cov_3.927281"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="P5292_09280"
     CDS             <1..183
                     /locus_tag="P5292_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRS39357.1"
                     /translation="GLTEYAGIRKDIVLFAYGNRIEVWSEEEYKKMLKESGDYGQLAE
                     EVLGKRKGGDERDLLP"
     gene            161..1084
                     /gene="rsmH"
                     /locus_tag="P5292_09285"
     CDS             161..1084
                     /gene="rsmH"
                     /locus_tag="P5292_09285"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRS39358.1"
                     /translation="MSATSYHEPVLLKESVDALDILADGIYVDATLGGGGHTREILRR
                     LGAKGKLFVFDRDEAAGANLPDDPRVVWVRHNYRYAWRFLRYYGALPVNGLLADLGVS
                     SHQFDSGERGFSIRFDGPLDMRMANASELTAADVVNRYPEDRLLKVFSDYGEIQNARQ
                     LVRKILAARNLQPLTTTGQLREVVLTVADRARESQYLARVFQALRIEVNDELASLRDL
                     LEQSPKLLAPKGRLVFITYHSLEDRLVKRFIADGQFEGEVEKDVYGNIPQTPFRALTR
                     KPLEPSAEELERNPRARSARLRIAIRTDQNG"
     gene            1077..1490
                     /locus_tag="P5292_09290"
     CDS             1077..1490
                     /locus_tag="P5292_09290"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HRS39359.1"
                     /translation="MAENKLRKSAEVEDEPEAGERKPRTRKSSRRFFKMMNVFGMFDR
                     DQVVHAMPFIGFVTLLTIGYIANSYYAERVVREIGKTKKDIRELNAEYISTSSELMYR
                     SRPSEVAKAVEHMGLKEPVDPPGKIVINQQPSKND"
     gene            1505..>2269
                     /locus_tag="P5292_09295"
     CDS             1505..>2269
                     /locus_tag="P5292_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HRS39360.1"
                     /translation="MSKKRDIFWRINLAFLLLIVFGLVIIGQIVRIQFVEGEQWKDLA
                     RSQSLKYQQIEASRGNIYGSDGKMLAMSTPIYDVRMDTRADGLTQELFDAKIDSLAMG
                     LAAIFGDRSRADYKQDLKRARAEGARYFLIRRNVGYRDLQRVKALPIFRMGRNKGGLL
                     VEQRNVRVLPYLDLAARTIGTLRNVKPVGIEAAFDDYLKGVSGKRLMQRISSGDWMPV
                     TDKDEIEPHDGNDVHTTIDVNIQDVAESSLRRHLELN"
BASE COUNT          521 a          568 c          715 g          465 t
ORIGIN      
        1 gggttgacgg aatatgcggg catccggaag gacatcgtcc tgttcgcgta tggcaaccgg
       61 attgaagtgt ggtcggaaga ggagtacaag aagatgctga aggaaagcgg tgattacggc
      121 cagttggcgg aagaggtgct gggaaaacgg aaaggcggag atgagcgcga cctcttacca
      181 tgagccggta ctgttgaagg aaagcgttga cgcgctggat atactggcgg acggcattta
      241 tgtcgacgcc actttgggag gaggaggcca tacgcgggag atcctgcgca ggcttggggc
      301 gaaagggaag ttgtttgttt tcgatcgtga tgaagcggcg ggtgcgaatc tgccggacga
      361 cccgcgggtc gtgtgggtcc ggcacaatta ccggtatgcc tggcggtttc tccgttacta
      421 cggagcgcta ccggtgaacg gattgctggc cgatctggga gtttcttctc atcagtttga
      481 ttcgggtgag cggggatttt ccatccgttt cgacgggccg ctggacatgc gcatggcgaa
      541 cgcatcggag cttaccgctg ccgatgtcgt gaaccggtat ccggaagacc gcttactcaa
      601 ggtgttctcg gattatggcg agatccagaa cgcgcgacag ttggtcagga agatcctggc
      661 ggcacgcaac ctgcagccgt tgacgacaac cgggcaattg cgcgaggtgg tgttgacggt
      721 agccgaccgg gcacgggagt cgcaatacct ggcccgtgtg tttcaggcct tgcgcattga
      781 agtgaacgac gagctcgctt cgctgcgtga cctgctggag cagtcgccta agctccttgc
      841 tccaaaaggc cgactggtgt tcatcacgta ccactctctt gaagaccggc tggtgaagcg
      901 ttttatcgcg gacggacaat tcgaaggaga agtggagaag gatgtttacg gaaacatacc
      961 gcaaacgcct tttcgggcgc tgacccggaa gccgctggaa ccttcggcgg aagaattgga
     1021 acgaaatccc cgggcgcgca gtgcgcgact ccggatcgca atacgaactg atcaaaatgg
     1081 ctgagaacaa actcagaaaa agtgcagaag tagaggatga gccggaggcc ggagagcgta
     1141 aaccgcgcac ccgaaagtcg tcccggcgct ttttcaagat gatgaacgta ttcggcatgt
     1201 tcgaccgcga ccaggtcgtg cacgcgatgc cgttcattgg ctttgtgacc ttgctgacca
     1261 tcggatacat cgcgaacagc tattacgctg agcgggtcgt tcgggaaatc ggaaagacga
     1321 agaaggatat ccgggagctc aatgccgaat acatctcgac cagttctgaa ttgatgtacc
     1381 gcagccgtcc ttccgaagtg gcgaaagccg tggagcatat gggtcttaag gaaccggtgg
     1441 atcctcccgg aaaaattgtg atcaatcaac aaccttcaaa gaacgactga tcgggacgag
     1501 ccgtatgagc aagaagcgcg acattttctg gaggatcaac ctcgcgttcc tgttgctgat
     1561 cgtattcggt ctggtcatca tcgggcagat cgtacggatc cagttcgtgg aaggcgaaca
     1621 gtggaaggac ctcgcccgta gccagtcgct caagtatcag cagatcgagg cgtcccgcgg
     1681 caatatctat ggtagcgacg gcaagatgct ggccatgtcc acgccgatct acgatgtccg
     1741 catggacaca cgtgccgacg gtctgacaca ggagctgttc gatgccaaga tcgattcgct
     1801 ggccatgggt ttggcggcca ttttcggtga ccgctcccgt gccgattaca aacaagacct
     1861 caaacgcgca cgcgccgaag gcgcccgtta ctttttaatt cggagaaatg tcggctatcg
     1921 tgacctgcaa cgggtaaagg cgctcccgat ttttcgcatg ggtcgcaata aaggtggatt
     1981 gctggtggag cagcggaatg tccgtgtgct gccgtacctc gaccttgccg cacggacgat
     2041 tgggacgctt cgcaatgtga agccggtcgg catcgaagca gcgtttgatg attacctgaa
     2101 gggcgtcagc gggaaacggt tgatgcagcg catctccagc ggtgattgga tgcccgtaac
     2161 ggacaaggat gaaatcgagc cgcacgacgg aaatgatgtt cacactacga tcgacgtcaa
     2221 catccaggat gttgcggaga gttccttgcg ccgccacctg gaactcaac
//
LOCUS       DAOTON010000473         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37202_length_2265_cov_2.485068, whole genome shotgun sequence.
ACCESSION   DAOTON010000473 DAOTON010000000
VERSION     DAOTON010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37202_length_2265_cov_2.485068"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="P5292_09300"
     CDS             <1..474
                     /locus_tag="P5292_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39361.1"
                     /translation="VSCQTSDEIRVFRLSDDSLLKIIPVGTKPQEFAESTNQPYLFVT
                     CTEEVVDANRKGLVYVINYNTLDLVDSIYTGYQPHGIDFDPVRKLVYVANLNVDNTGP
                     APHHVSDCGGRNGYITIIDEQTLELYRRSFPDGSTSSYKNEVLRAPYFVAYRKRP"
     gene            complement(480..932)
                     /locus_tag="P5292_09305"
     CDS             complement(480..932)
                     /locus_tag="P5292_09305"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HRS39362.1"
                     /translation="MMNLFDRVRVPFHLQSIEEEGYHILVDAVINKQPARLLIDTGAS
                     RSVFDTNRFSRFVEAHEFRPDEKLSAGLGTSSMQTMKVTLTELRIGECSIPGFETILL
                     DMSHVNSSYERLGLPQLDGVIGSELLVLLEAQIDYRKRQLIIKRRIGK"
     gene            complement(1010..1954)
                     /gene="mdh"
                     /locus_tag="P5292_09310"
     CDS             complement(1010..1954)
                     /gene="mdh"
                     /locus_tag="P5292_09310"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRS39363.1"
                     /translation="MKVTVVGAGNVGATCADVIAQKDFVHEVVLLDIKEGVSEGKSLD
                     MWQTSPINLYDTRLIGSTNDYSKTADSDVVVITSGLPRKPGMSRDDLIATNAGIVRSV
                     TENIIKYSPNTIIIVVSNPLDVMTYCAYLTAKIDSRRVFGMAGILDTARYRAFLAEAL
                     NTSPKDIQAVLMGGHGDTMVPLPRYTTVAGIPVTELIAKDKLDAIVERTKKGGGELVN
                     LMGTSAWYAPGAAAAQMVEAVIKDQKRIFPCCAWLNGEYGLKGVYLGVPVKLGRNGIE
                     EIIELKLTEEEKSLVSASAKAVKEVMDVLDNMQQPAVK"
BASE COUNT          530 a          584 c          574 g          577 t
ORIGIN      
        1 gtcagttgcc agaccagtga tgagatacgc gtctttcgtc tttcggatga cagtctgttg
       61 aagatcattc cggtcggcac caagccgcag gaattcgccg aatccaccaa ccaaccttac
      121 ctttttgtca cctgtacgga agaagtcgtg gatgccaacc gaaaggggtt ggtgtatgtg
      181 atcaattaca acaccctcga cctggtcgat tccatctata ccggatatca accacatgga
      241 attgatttcg atccggttcg caagctggtg tatgtcgcca atctaaatgt ggacaatacg
      301 ggtccagccc cgcatcacgt gtcggattgt ggcgggagga acggctacat tacgatcatt
      361 gacgaacaga cgcttgaact atatcgcaga tcgtttcccg acgggtccac cagctcttac
      421 aaaaacgaag tgttgcgggc gccgtatttt gtcgcttacc gtaaacgccc ctgaggcgct
      481 tacttcccaa tccttctctt tatgatcaat tgccgcttcc ggtagtcgat ctgcgcttcc
      541 aacagtacga gcagttcact cccgatgacc ccatccagtt ggggtaagcc cagccgttca
      601 taggaactgt tgacatgact catgtcgagc aggatcgtct caaagccggg tatcgaacat
      661 tcaccgatcc gcagctctgt cagcgtcacc ttcatggttt gcatggaact ggtgcccagg
      721 ccggcggata gtttctcatc cggccgaaac tcgtgtgctt cgacaaatcg gctgaagcgg
      781 ttcgtatcga ataccgaacg ggaagcgccg gtatcgatca gcaaccttgc cggttgcttg
      841 ttgatgacgg cgtcgacgag gatatgatac ccttcttcct cgatggattg caaatgaaaa
      901 gggacacgaa cacgatcaaa taagttcatc atcttttctt tggttcagca gtcttggaaa
      961 ataaaaaaga gcacccgttc acggatgctc tttcgttgat gtcgaaggat cactttacgg
     1021 caggttgctg catgttatcg agcacgtcca tgacctcttt cacggccttg gccgatgcgc
     1081 tcacgagcga tttttcttct tcggtgagtt tgagttcgat gatctcctcg ataccgttcc
     1141 gtcccagttt aaccggaact cccaggtata cgcccttcag gccgtactcg ccattcagcc
     1201 atgcgcagca agggaagatc cgcttctgat ccttgatgac cgcctccacc atttgggcag
     1261 cggctgcacc cggggcatac caggcggaag tacccatgag gttgaccaat tcgcctccgc
     1321 ccttcttggt gcgttcgacg atggcgtcga gtttgtcctt tgcaataagt tccgtcacgg
     1381 gaatccctgc aacggttgta tagcgcggca gcggaaccat cgtatcgcca tggccgccca
     1441 tcaataccgc ctggatgtct ttgggcgaag tgttcaacgc ttcggccagg aacgcacggt
     1501 agcgggctgt atccaaaata cccgccatac cgaaaacgcg acgagagtcg atcttggccg
     1561 tcaggtaagc gcaatacgtc attacatcga gcggattgga tacaacgatg atgatcgtgt
     1621 tgggactgta tttgatgatg ttctccgtta cggaacgaac gatgccggca ttggtcgcga
     1681 tcagatcgtc gcggctcata ccgggcttgc ggggcagacc ggaggtgatg acgacaacat
     1741 ccgagtcggc cgtctttgaa taatcgttgg tggagccgat cagacgcgta tcgtacaggt
     1801 tgatcggact ggtttgccac atgtcaagcg acttcccttc tgacacgccc tctttgatat
     1861 caagcagaac gacttcgtgc acgaaatctt tctgcgcaat aacatcagca caagtggcac
     1921 ccacgttacc ggcgcctacg acagttactt tcatggatta tggggttttc cgaattggtt
     1981 gtttctgaga ttaacggagc ccaaaagtac gaactccggg aggataccga aatggcaaaa
     2041 atcatccgat atcgatggga ggttgtggaa tttgatcgaa aagggctgtg tttagacttg
     2101 cggggaattt tgtgaactgt tgataagtgg ggactaaaaa attgcttttt tcgaaggatt
     2161 tcactaaatt tcgttttaac aaccctctgc cactaaaagt tacattttat tgaaaattcc
     2221 agcattgttt gattgtcttt gcccaaaaga cgattctctg agccg
//
LOCUS       DAOTON010000474         2260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37301_length_2260_cov_2.952381, whole genome shotgun sequence.
ACCESSION   DAOTON010000474 DAOTON010000000
VERSION     DAOTON010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37301_length_2260_cov_2.952381"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="P5292_09315"
     CDS             complement(<1..616)
                     /locus_tag="P5292_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39364.1"
                     /translation="MDILPSVIHRMTRDEVRFFKLFSGRSHDRADRLDKRLFDLIRQA
                     GEEYDDDKVFERLYEEGNKNAYYRLKNRLLQDVGKSLTLQHFDDDDTVHALHLLGLAK
                     FHLGRSNTRAAHYYLRRAESEAVKLNQYELLDLIYGDLIRLSHEQVSVNPEEYIKRRR
                     ENLERIHQVRAIDDLLAAVSYRMRVTQNFGGGKNPVMPLLEKIVN"
     gene            711..1403
                     /locus_tag="P5292_09320"
     CDS             711..1403
                     /locus_tag="P5292_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39365.1"
                     /translation="MKRLLTSAIYVLIVGLTNLTTLYAQAPRLTITSITGIPTQQGDT
                     ITDTTRYTFSAVVQNTGGGAFQGAIDILAIANPQLVDTLAGDSANFILTPGSAATLSN
                     PNLQFRPTYYDGGDNIIVVWPAARGMVVPADSITLQLYYLRLTQTAPETRLEPIRIRP
                     NPATDHILLETGEIKSLEWVRIYDTRGAMVRSIPRPESSLIQLGDLPAGLYLLEITQS
                     NGARLSTRLIKE"
     gene            1554..>2260
                     /locus_tag="P5292_09325"
     CDS             1554..>2260
                     /locus_tag="P5292_09325"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRS39366.1"
                     /translation="MNQPQRPYCNDLLHYSRFVTREVRIGELLMGAHHPIRVQSMTTT
                     DTMDTAATVAQSIRMIEAGCELVRITAPSVNEAKNLENIKAELRKRGYATPLVADIHF
                     TPNAAEVAARIVEKVRVNPGNYADKKKFESIDYTDDAYEAELDRIRERFTPLVRICRE
                     YGTAMRIGTNHGSLSDRILSRYGDTPLGMVESAMEFLRICEAENFHQIVLSMKASNTQ
                     VMVQAYRLLVATMREHE"
BASE COUNT          479 a          688 c          624 g          469 t
ORIGIN      
        1 cgttgacgat cttttcgagc agcggcatga ccggattttt tccgccgccg aaattctggg
       61 tcacgcgcat gcggtagctg acggccgcga ggagatcatc gatcgcgcgc acctggtgga
      121 tacgctccag gttctcgcgg cggcgtttga tgtactcttc gggattcacc gagacctgct
      181 cgtgcgatag gcggatgaga tcgccgtaga tgaggtcgag caattcgtac tggttcagct
      241 tcacggcctc cgactccgcc cggcgcaggt agtaatgcgc cgcccgggtg ttgcttcgtc
      301 ccaggtggaa cttcgccagt ccgagcaaat gcagcgcgtg gacggtatca tcgtcgtcga
      361 agtgctggag tgtgaggctc ttgccgacat cctgcaagag gcggttcttg agccggtaat
      421 aagcgttctt gtttccttcc tcgtacaaac gttcgaacac cttgtcgtcg tcgtactcct
      481 ccccggcctg tcggatgaga tcgaaaagtc gcttgtcgag ccggtcggca cgatcgtgac
      541 tgcgaccgct gaacagcttg aaaaacctca cttcatcgcg ggtcatgcgg tgaatgaccg
      601 aaggcaggat atccatgata aacaggcttt gaacgcgtaa ggaaatttac aaattctgtg
      661 ggaaaccgcc aaatcccgcc gtacttttga tttgggttaa taccaacgct atgaaacggc
      721 tcctcacctc tgcaatttac gtgttgatcg tcggcctgac gaacctcacg accctatacg
      781 cccaggctcc ccgcctgacc atcacgtcga tcacggggat accgacgcaa cagggcgata
      841 ccattacgga cactacgcgc tacaccttca gtgccgtggt ccagaacacc ggtgggggag
      901 cctttcaggg cgccatcgac attctggcca tagcgaaccc gcagctcgtc gacactctgg
      961 ccggcgactc ggccaacttc atcctcaccc ccggcagcgc ggccacctta tccaatccca
     1021 acctgcagtt ccgtcccact tactatgacg gaggcgacaa catcatcgtc gtttggccgg
     1081 cagcgcgcgg catggtggtt cccgccgaca gcatcaccct gcaactgtat tacctgcggc
     1141 tgacccagac ggctcccgaa acccgtctgg aaccgatccg catccgtccg aacccggcga
     1201 ccgatcatat cctgctcgaa accggcgaaa ttaaatccct tgaatgggta agaatctacg
     1261 atactcgcgg ggcgatggtc cggagcatcc cgcgtccgga atcgagcctg atccagctgg
     1321 gtgacctgcc ggccggactg tatcttttgg agatcactca aagcaacggc gcgcggttga
     1381 gcacgcgtct gatcaaagaa tagttccagt tttgtgtttc agtggtgtgt cactgcgtgt
     1441 tccccgcagt accaaagccg ccggtcgttt tccggcggct tttttcgtgg cctgcccgta
     1501 ccggtggcct gaaccattaa cccctatctt tgtttcaaga ttttcaggtt cccgtgaatc
     1561 agccgcaacg tccttactgc aacgatctgc tacactactc ccgcttcgtg acacgagagg
     1621 tcaggatcgg tgaactttta atgggcgctc atcacccgat ccgggtgcag tcgatgacca
     1681 cgaccgacac gatggacacc gccgccaccg tcgcgcagag tatccggatg atcgaagcag
     1741 gctgcgagtt ggtgcggatt acggcgccca gtgtgaacga ggcgaagaac ctggaaaaca
     1801 tcaaggcgga actgcgcaag cgcggttatg ccacaccgct cgtcgccgac atccacttca
     1861 ccccgaacgc ggccgaagtc gccgcgcgga tcgtggaaaa ggtgcgggtc aatcccggca
     1921 attacgctga caagaagaaa ttcgaatcca tcgattatac cgatgacgcc tatgaagccg
     1981 aactggatcg tatccgggaa cgctttaccc cgctcgtgcg catctgcagg gaatacggta
     2041 cggccatgcg catcggcacc aaccacggtt cgttgagcga ccgcatcctg agccgctacg
     2101 gcgacacgcc gctgggcatg gtggaatcgg cgatggaatt cctccgcatc tgcgaagcgg
     2161 aaaacttcca ccagatcgtg ctttcgatga aagccagcaa cacccaggtg atggtacagg
     2221 cgtaccggtt gcttgtcgcg accatgcgcg agcacgaaat
//
LOCUS       DAOTON010000475         2258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37343_length_2258_cov_2.830232, whole genome shotgun sequence.
ACCESSION   DAOTON010000475 DAOTON010000000
VERSION     DAOTON010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37343_length_2258_cov_2.830232"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..943
                     /locus_tag="P5292_09330"
     CDS             98..943
                     /locus_tag="P5292_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39367.1"
                     /translation="MKYRKIMDQITLHRLIQEHLERMRDLHRSMQLHTGSIPSSEIDR
                     FLAELRGTYELALTLYHRNAIRSMDDLEAVIGNRYTAESGPLREIEQRAGERMMGSFN
                     MPKETSPEAVPTKEPVEETPAPTPEAVAATPSTQHISSDQLMVDAMTRAEELQSRATR
                     DLNEKFEEQPTLADRFEEKTTLAERIAPSNSSARVAEKMQRKPIRDLKAAIGINERFL
                     FINHLFAGDSNEYHKAIDTVNGAADIQAARKFVEEQLIARYKWNLSGDPADAFMDLLE
                     RRFLA"
     gene            952..>1600
                     /gene="rsmI"
                     /locus_tag="P5292_09335"
     CDS             952..>1600
                     /gene="rsmI"
                     /locus_tag="P5292_09335"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRS39368.1"
                     /translation="MAKLVLVPTPIGNLEDITLRAIRVLTDVDLVLAEDTRTSGNLLK
                     HLGLSKRLQSYHAHNEHRVVEELVKRIELGQSVALITDAGTPGISDPGFLLVRECLRQ
                     GVEVETLPGATALIPALVNSGLPCDRFCFEGFLPQKKGRQKRLEQLQSEERTLVFYES
                     PYRLVRALEEFVQHFGKDRLASVSRELSKLHEENKRGTLEELLAYCDKLPGWPERV"
     assembly_gap    1601..1610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1697..>2258
                     /locus_tag="P5292_09340"
     CDS             1697..>2258
                     /locus_tag="P5292_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39369.1"
                     /translation="MHRLLILLLSALLHTGISEAQPVVSYCKRRPIDTTIPSSLVSRN
                     LDLLHQECRFRVDPQVAAISGSVRSTFRTTAIVDAIEFDASDALVVDSVVYHGIRLPA
                     IRPGDDLIRIELPATLANGSYDSIDVHYHGTPSSGGFGSFETAQHDGQPVLWTLSEPY
                     GARDWWPCRQTLDDKIDSLDVIVTSPA"
BASE COUNT          515 a          683 c          609 g          441 t
ORIGIN      
        1 gccaagatga atgcgtccgg gagacacgat acacgggggt ttgttcgtta ttcagccaac
       61 cagcggcctt tccggtcggg gcgttacgta actttacatg aagtaccgaa aaatcatgga
      121 ccaaatcacc cttcaccgcc tcatccagga acacctggaa cggatgcgtg atcttcaccg
      181 cagcatgcag cttcataccg gcagcatccc ctcatcggag atcgaccggt tccttgccga
      241 attgcgcggg acctacgaat tggcgctgac cctctaccac cgcaacgcca tccggagcat
      301 ggatgatctc gaggcggtaa tcggaaaccg ctatacggcg gagtcgggcc ccttacggga
      361 gatcgaacaa cgcgcaggcg aacgcatgat gggtagtttt aacatgccaa aagaaacgtc
      421 gcccgaagct gtgccaacga aagaacccgt cgaagaaacc ccggctccga cgcctgaagc
      481 cgtagcggcc accccgtcca cgcagcacat ttcctccgat cagttgatgg tggatgccat
      541 gacgcgtgca gaagaacttc agtcacgcgc gacacgcgac ctgaacgaga aattcgaaga
      601 acagccgacg cttgccgacc ggttcgaaga gaagaccacc ctggctgaac gcatcgctcc
      661 ttcgaattcc tccgcgcgcg tcgcggaaaa gatgcaacgt aaaccgatcc gggatttgaa
      721 ggcagcgatc gggatcaacg agcgttttct tttcatcaat cacctgtttg caggcgattc
      781 gaatgagtac cacaaagcca ttgacacggt caatggtgcc gccgacatcc aggccgcgcg
      841 caagttcgtg gaagagcaat tgatcgcccg gtataagtgg aacctgagcg gtgatcccgc
      901 cgacgcgttc atggacctgc tcgaacgccg tttcctggcc tgacacccgg catggcaaaa
      961 ctggtgctgg tacccacgcc tatcggcaac ctggaagaca tcaccctccg ggctatccgt
     1021 gtactgaccg acgtcgacct ggttctcgcc gaggacaccc gcaccagtgg caacctgttg
     1081 aagcaccttg gcctgagcaa gcgcctccaa tcgtaccatg cgcacaacga acaccgggtg
     1141 gtggaagaac tggtcaagcg cattgagctg ggacaatccg tagcccttat caccgatgcc
     1201 ggaactccgg gcatctccga tccgggtttt ttactcgtgc gggaatgcct gcggcaaggc
     1261 gtagaagtgg aaaccttacc gggagccacc gcgctgatac cagcactggt caatagcgga
     1321 ctaccctgtg accggttctg ctttgaagga tttcttcctc aaaaaaaagg acgtcaaaaa
     1381 cggctggaac aattgcagtc ggaagagcga accctggtct tctatgagag cccgtatcga
     1441 cttgtccggg cgttggaaga gtttgtccag catttcggaa aagaccggct ggccagcgta
     1501 tcccgtgaat tgagcaagtt gcacgaagaa aacaaacgcg gcacgctcga ggagttgctc
     1561 gcctattgcg acaagcttcc cggatggccc gagagggtca nnnnnnnnnn tcgccggtgc
     1621 acgctatcag gccggtactg ccccttccga gacggaatcg gattgaccgt tcttcatcgg
     1681 cgactgatct aataccatgc atcgcctcct catcctgctg ttgtccgcgc tcctgcatac
     1741 aggaatatcg gaggcgcaac cggtcgtgag ctattgcaaa cgccgaccga tcgataccac
     1801 tattccctcc tcacttgtca gccgcaacct ggatcttttg caccaggaat gcagattccg
     1861 ggtcgacccc caggtggccg caatcagcgg atcggtcaga tcgacttttc gaacaacagc
     1921 gatcgttgat gcgatcgagt tcgatgcctc ggatgcgctt gtggtcgata gcgtagtgta
     1981 tcacggtatc aggcttccag caattcgtcc cggcgacgac ctgattcgca ttgaacttcc
     2041 cgcaacactg gccaacggct cctatgattc gatcgacgtc cactatcacg gcaccccttc
     2101 ctccggcgga ttcggttcat tcgagacggc gcaacacgac gggcaaccgg tgttgtggac
     2161 cctgtcggaa ccttatggcg cccgcgactg gtggccctgc cggcagaccc tcgacgacaa
     2221 gatcgactcg ctcgatgtta tcgtcaccag cccggccg
//
LOCUS       DAOTON010000476         2256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37389_length_2256_cov_3.170377, whole genome shotgun sequence.
ACCESSION   DAOTON010000476 DAOTON010000000
VERSION     DAOTON010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37389_length_2256_cov_3.170377"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..708)
                     /gene="smpB"
                     /locus_tag="P5292_09345"
     CDS             complement(265..708)
                     /gene="smpB"
                     /locus_tag="P5292_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRS39370.1"
                     /translation="MTDYTSVIKNRKAGFEYHLLQQFTAGMVLTGTEVKSLRAGKASI
                     AEAFCIVQDDEVWVKNMHIAEFERGSYYNHDPRRLRKLLLNRQEIRKITVRLKEKGIT
                     IIPVQVYFSEKGLVKMDIALAKGKKLYDKREDLKKKDMDRERKEG"
     gene            complement(753..1889)
                     /locus_tag="P5292_09350"
     CDS             complement(753..1889)
                     /locus_tag="P5292_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRS39371.1"
                     /translation="MSTKLHLLYIALFLSACDQPTEGRKESAPEYSADQIVSLTAAQL
                     RQANIQIALPDRRTMSQEITLNGMIDVPPQNLVSLSFPYGGFVRKLSLLPGMHVQKGE
                     SIAVLEDAAYVGLQRDFLVARSRLEYLRLDKERQEKLRTEDAVSEKTYQQIRNEFDMQ
                     QAEVAAASEKLRLLGLDPASFDASQVSRQVSLHSPIDGYVASVNVNTGKAVQPTDVLF
                     ELVDPKDLHLTLTVFEKDIDRIEVGQRVWAVRNDAPADTLQAEVILVSHNLDAQRKGL
                     VHCHLLETKGRLLPGMFMRAGIRLAGRTATVVPEEAVVRYGTEQFVFEAINDSTFRMH
                     AVKTGVREDGWIELLTEEDDLAVKRLVIRSAYALLGKAKNVMEE"
     gene            complement(1898..>2256)
                     /locus_tag="P5292_09355"
     CDS             complement(1898..>2256)
                     /locus_tag="P5292_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39372.1"
                     /translation="IPLFYWGARARVKSAKANWAQAGFLQEAEQIRLRRERLERQERL
                     QAAGEQYSIYRDQTLPLISELERKAALRLSSGDIRYFEWLSIVEQCLRTRHAALESYR
                     AYLHAIIDWNELSEQN"
BASE COUNT          473 a          626 c          575 g          582 t
ORIGIN      
        1 cgaccggcga aggcgaatct accgttgagt tctggaatgg taccggtacc accagccaga
       61 ctggcaagac cggcaaatcc agctctttcg cagttgacgt agacaacgct agcggcgcta
      121 tcgttctctc cctctacttc taagagaacc tgcccataaa aaaaacgccc cggatcccgg
      181 ggcgtttttt tttattccta aaatcaatca ccaatcacta atttttaatt actagtttct
      241 agtttctagt ttctagtttt tcaattaccc ctctttccgc tccctatcca tatccttctt
      301 cttcaaatcc tcccgtttgt cgtacagctt tttacccttg gcaagcgcaa tatccatttt
      361 cacgagtcct ttctctgaga agtagacttg cacgggaatg atggtaatgc ctttctcctt
      421 caggcggacc gtgatctttc gtatctcctg acgattcagg agcaattttc gcaaccgcct
      481 ggggtcatgg ttataatagg agccccgctc aaattcggcg atgtgcatgt tctttaccca
      541 cacttcgtcg tcctggacga tgcaaaacgc ttctgcaatg cttgcttttc cggcacgcag
      601 ggatttgact tccgttccgg tcaataccat cccggccgtg aactgctgca acaggtggta
      661 ttcgaatccc gctttgcggt tcttgatgac ggaagtgtag tcggtcatgg gttggcgaag
      721 atacagagtc cggccgcaag ctgcggcctt gatcattctt ccatcacatt ctttgcctta
      781 cccaagaggg cgtaggcact ccggataacc agccttttga cggccagatc atcttcttca
      841 gtaagcagtt cgatccatcc atcctcccga actccggtct tgaccgcatg catccggaac
      901 gtggaatcat tgatggcttc gaagacgaac tgttccgtcc cgtagcgtac gaccgcttcc
      961 tcgggaacaa ccgttgccgt tctacccgca agccggatac cggcccgcat gaacataccg
     1021 ggcaataatc ggcctttggt ctccagcaaa tggcaatgca caagcccttt gcgctgcgcg
     1081 tccaggttgt gactgaccag gatcacttcg gcttgaagtg tatccgccgg ggcgtcgttt
     1141 cgcaccgccc agacccgctg gccgacttcg atgcggtcga tgtccttttc gaatactgtc
     1201 agggtcagat gcaggtcctt ggggtccaca agttcaaaga gtacgtcggt aggttgtacg
     1261 gctttaccgg tgttgacgtt gacagaagcc acgtatccgt cgatcggcga atgcagactg
     1321 acctgacgag acacctggct ggcatcgaag gaggcaggat ccaatccgag caggcgcagc
     1381 ttttccgaag ctgctgctac ctcggcctgc tgcatgtcaa attcatttcg gatctgttgg
     1441 tacgtcttct cgctcacggc atcctcggtt cgcaattttt cctggcgttc cttgtccagg
     1501 cgaaggtatt ccagccggga gcgggcaacg aggaaatcac gctgcaagcc gacataagcg
     1561 gcatcttcca ggacagcgat cgactcacct ttctgaacat gcatgcccgg aaggagggat
     1621 agtttccgca caaatccacc atatggaaag ctgagtgaaa ccaggttctg aggaggcacg
     1681 tcgatcattc cgttcaacgt gatctcctgg ctcatggtgc ggcggtcagg tagcgcgatc
     1741 tgaatatttg cctgccgtaa ttgcgcggct gtcagcgata cgatctgatc ggcggagtac
     1801 tcgggggcac tttcttttct tccttcagta ggttgatcgc aggcggaaag gaatagggcg
     1861 atgtatagga ggtgcaattt ggtgctcatg gtcttgttca gttttgttca gataactcgt
     1921 tccagtcgat gattgcgtgc agataggcgc gataggattc taatgcagcg tgacgggtcc
     1981 gcaggcattg ttccacaata ctcagccatt caaaataccg gatatcgcca ctgctgagcc
     2041 gcagcgccgc tttgcgctct agttcggaga tcaacggcag tgtctgatcg cggtagatcg
     2101 aatattgctc gccggcagcc tgtaggcgtt cctgccgctc gagtcgttca cgccggagcc
     2161 tgatctgttc tgcttcctgc aggaatccgg cttgcgccca attggcttta gcacttttga
     2221 cacgggcacg tgcgccccag taaaacaagg gtatac
//
LOCUS       DAOTON010000477         2254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37428_length_2254_cov_3.245566, whole genome shotgun sequence.
ACCESSION   DAOTON010000477 DAOTON010000000
VERSION     DAOTON010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37428_length_2254_cov_3.245566"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="P5292_09360"
     CDS             complement(<1..283)
                     /locus_tag="P5292_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRS39373.1"
                     /translation="MKPWYETWFDSPYYHILYHHRDGTEAAAFLDRLIGQLPMRSGAR
                     VLDVACGKGRHSIHLNKRGFDVCGFDLSKASIAHNRQFENDRLHFFVHDM"
     gene            417..1544
                     /gene="corA"
                     /locus_tag="P5292_09365"
     CDS             417..1544
                     /gene="corA"
                     /locus_tag="P5292_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRS39374.1"
                     /translation="MKRPSSKKRHRARRARHAPPGTPPGNILVEEGALKPRITGFFYT
                     PDFFDERPFNSLSEATAAIQSYPDRVTWIDVQGFGSRAFMEELATAFGIHRLQIEDVV
                     NQYQRPKADEYDQHLFLISRIFREAETGLSNDQLSIFLGRNMVLSIQDRYDNVLDPVR
                     DRIRHGKGFVRKHGADYLAYALMDVALDNLYPILEHVGDRLDELQDELLLRPTREALN
                     RILQIKRELIEIRRSIWSERDKVNDILRSAFPQVQEATKIFFRDTYDHSIQILDLVES
                     YREVTASLVDVYHSSVSQRMNQVMKVLTIISTIFIPLTFIVGLYGMNFTDVHPVTGKV
                     MPLNMPELYMPYGYVTVLLVMVVIVILQLIFFYRKGWLSKE"
     gene            1546..>2254
                     /locus_tag="P5292_09370"
     CDS             1546..>2254
                     /locus_tag="P5292_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRS39375.1"
                     /translation="MEAPLVIKTFAGLESLLAEEVTALGASGAKAGKRAVYANGDLKT
                     VYRINLCSRYALRVLQEVHRFSAPDEKALYAGVQEVEWNQFLPVDGSLAVDAVAYRSN
                     MDHTQYIALKTKDAIVDQFRDAYGKRPNVDIQRPTVRIHVHLVENNATVSLDSSGDSL
                     HRRGYREEPAGAPLSEVLAAGLIGLTGWKGDRPLYDPFCGSGTLLTEAVLLARRIAPG
                     RFREGFGFQRWMNFDAGL"
BASE COUNT          480 a          637 c          659 g          478 t
ORIGIN      
        1 gcatgtcgtg cacgaagaag tgcaaacgat cgttttcgaa ttggcgattg tgcgcgatgc
       61 tggctttgga aaggtcgaag ccgcatacat caaatcctcg tttgttcagg tgaatggaat
      121 gccgaccttt tccacaggct acgtcgagca cccgggcccc tgatcgcatc ggcagttgtc
      181 cgatcaagcg gtccaggaac gcggccgctt ccgtaccatc acggtgatgg tacaggatgt
      241 ggtagtaggg tgaatcgaac caggtttcgt accagggttt catgcgtcgc gaagttcggt
      301 tttttcggcc ttgccaaatg ggcgggaatg gagggctttc aacaaacgtg catggattat
      361 ttgagcgtgt gcctgggttc gagaaacgaa tatttgtaaa tctttaacct cccgtcatga
      421 agcgccccag cagtaagaag cgtcaccgcg cccgtcgcgc gcgacacgca ccgcccggta
      481 ccccgcccgg caacatcctg gtagaggagg gcgcgctcaa gccccggatc acgggctttt
      541 tctatacccc cgactttttc gacgaacgac ccttcaacag tttgtccgaa gctaccgccg
      601 cgatccagtc ctatccggac cgggtcacct ggatcgatgt gcagggtttt ggcagccgtg
      661 ccttcatgga agagctcgcc accgcattcg gcatccaccg cttgcagata gaagacgtcg
      721 tcaatcagta ccagcggccc aaggccgacg aatatgatca gcacctgttc ctgatctccc
      781 gcatttttcg cgaagcggaa acgggtttga gcaacgatca actgagcatt ttcctgggca
      841 ggaacatggt cttgtccatc caggaccggt acgacaatgt gcttgatccg gtgcgtgacc
      901 ggatccggca tggaaaaggg ttcgtgcgca agcatggggc cgattacctg gcctacgcgc
      961 tcatggatgt cgcgctggac aacctgtatc cgatcctgga acacgtaggc gaccgcctcg
     1021 acgagttgca ggatgaattg ctgctgcgac caacgcgcga ggcgctgaac cgtatcctcc
     1081 agatcaagcg cgagctcatc gagatccggc gtagcatctg gagtgagcgg gataaggtga
     1141 acgacatcct gcggtcggct ttcccgcaag tacaggaagc cacgaagatc tttttccgtg
     1201 acacctacga ccatagcatc cagatcctcg atctggtcga gagttaccgg gaagtcacgg
     1261 catcgctggt ggatgtttat cattcgtcgg tgagccaacg catgaaccag gtgatgaaag
     1321 tcctgaccat catctccacg atcttcatcc cgttgacctt catagtcggc ctttacggga
     1381 tgaacttcac cgacgtgcat ccggttaccg gcaaggtcat gccgctcaac atgccggaat
     1441 tgtacatgcc gtacggttac gttacggtgt tgctggtcat ggtcgtgatc gtgatcctgc
     1501 aactcatttt cttttatcgc aaaggatggc tatcgaaaga atgatatgga agcacccctg
     1561 gtgatcaaaa ccttcgccgg actcgagtcc ctgttggcgg aggaagtaac ggccctcggg
     1621 gcaagcggag cgaaggccgg caaacgcgcg gtctatgcca acggcgatct gaagaccgtg
     1681 taccgcatca acctgtgcag ccgttatgcg ctgcgggtcc tgcaggaagt acatcgcttc
     1741 tccgctccgg atgaaaaggc cttgtatgcg ggtgtgcagg aagtggaatg gaaccagttc
     1801 ctgccggtgg acggctcact ggccgtcgac gcggtggcgt atcgatccaa catggaccat
     1861 acgcaataca tcgcactgaa gaccaaggac gcgatcgttg accagttccg cgacgcgtat
     1921 ggaaaacggc cgaacgtcga cattcaacgc ccgaccgtac ggatccacgt tcacctggtg
     1981 gagaacaatg ccaccgtttc gctcgacagc tccggggatt ccctgcaccg gcgcgggtat
     2041 cgcgaggaac cggcgggcgc gcccctgagc gaagtactcg cggcaggcct gatcggcctg
     2101 accggctgga agggtgatcg cccgctgtac gatccgttct gcggcagcgg caccttgttg
     2161 accgaagccg tcctgttggc gcggcgtatt gctccgggac gattccggga gggcttcggc
     2221 tttcaacgct ggatgaattt cgacgcagga ttat
//
LOCUS       DAOTON010000478         2251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37509_length_2251_cov_3.173042, whole genome shotgun sequence.
ACCESSION   DAOTON010000478 DAOTON010000000
VERSION     DAOTON010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37509_length_2251_cov_3.173042"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="P5292_09375"
     CDS             <1..627
                     /locus_tag="P5292_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRS39376.1"
                     /translation="WWSWSLVQRDREVRTLQEMPPRLLERVLGENGITLWKKANGIDN
                     SPVEPYSERKSISTEETFDQDTIDVRFLEALLVGMAERLAFQLRNENKLTACVTVKVR
                     YSNFDTHTMQCRIPYTSSDHTLIARAKELFAKLYNRRLLVRLIGLRFSHLVGGAQQID
                     LFEDSEEMVRLYQAMDRMRRRFGDSSVQRAAGQGVERRRFNPFNGQRL"
     gene            complement(828..2246)
                     /locus_tag="P5292_09380"
     CDS             complement(828..2246)
                     /locus_tag="P5292_09380"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRS39377.1"
                     /translation="MTGTYRATDSLILLLDDKSGLPTGLLSADEQAYVQHELKADRNN
                     VVINQYRRLIAVHRLDKKKTGAALLEACRRAGDALLGAFNRQKKERVVLVDVAGHGDT
                     ALALAEGMALGNYQFLKYRSKGKEKEAHTVSEILIQSTEVSRKAVEKLNHVIDAVYRA
                     RTLVNEPQSYLNATVFSSELAKLGRSAGIRVEVLKKARIKSLKMGGLLAVNQGSPQPP
                     TFTIMEYKPRGAKNRKPYVLVGKGVVYDTGGLSLKPTPNSMDYMKCDMAGGATVACAL
                     YAIAKAKLPVHVVALIPATDNRPGGDAYTPGDVVTMMSGQTVEVLNTDAEGRMLLADA
                     LHYAKKYKPELVMDFATLTGAAAAAIGPYGIVCMGTASEEVKARVKESGEQVYERMAE
                     FPFWDEYDDLLKSDIADMKNIGGPTAGAITAGKFLQRFVDYPYMHFDIAGPAWLQTNW
                     SYRGKNGTGMGVRMLVEFFSGV"
BASE COUNT          487 a          676 c          557 g          531 t
ORIGIN      
        1 tggtggtcct ggtcactcgt gcaacgcgat cgtgaagtgc gcaccctgca ggaaatgccg
       61 ccgcgactgc tcgaacgcgt gctcggagaa aacggcatca cgctctggaa aaaagccaac
      121 ggcatcgaca actcgcccgt ggaaccgtat tcggagcgca agtccatctc caccgaagag
      181 accttcgacc aggatacgat cgacgtccgc ttcctcgaag ccctgctcgt cggcatggcc
      241 gaacgtctcg ccttccagtt gcggaatgag aacaaactca cggcctgcgt cacggtcaag
      301 gtgcgctact cgaacttcga tacgcacacc atgcagtgcc gcatcccgta cacgtcctcc
      361 gaccataccc tcatcgcccg cgccaaggaa ctctttgcaa agctctacaa ccgccgcctg
      421 ctcgtccgcc tgatcggact acggttcagt cacctcgtgg gaggcgcgca acagatcgac
      481 ctgttcgaag acagtgagga gatggtccgg ctctaccagg ccatggaccg catgcgccgc
      541 cgcttcggcg actcctccgt ccaacgcgcc gccggccagg gcgtcgaacg aagaaggttc
      601 aatccgttca acgggcagag gctgtagggg ggggattggt aactggtaat tagtaactgg
      661 taattagtaa ctggtaatta gtaactggta attagtaatt aggttatttg cagtaaccag
      721 attctattcg ctcctgccta cgctaaagct tcggcaggta aaattcacta ttcactattc
      781 actattcact attcactatt cactattcac tgttcactat tcactcttca tactccacta
      841 aaaaactcca ccagcatccg aacacccata cccgtcccgt tctttccgcg gtagctccag
      901 ttggtctgta gccaggccgg tccggcgatg tcgaaatgca tgtaagggta gtcgacaaag
      961 cgttgcagga atttgcctgc ggtgatcgca ccagcggtcg gtccgccgat gtttttcata
     1021 tcagcaatgt cggatttcag caagtcgtcg tactcgtccc agaagggaaa ctcggccatc
     1081 ctttcataca cctgctcgcc cgattccttt acccgggctt tcacctcttc ggaagccgtt
     1141 cccatgcata caatgccata aggaccgata gccgcggctg cagcgccggt aagcgtggcg
     1201 aagtccatga cgagttcggg tttgtatttc ttggcataat gcagcgcgtc cgcaagcagc
     1261 atacgtcctt cggcatcggt gttcaatact tcaaccgtct gtccgctcat catcgtcacg
     1321 acatcgcccg gtgtataggc atcaccaccc gggcggttgt ccgttgccgg gatcagcgca
     1381 acaacgtgta ccggaagttt cgctttcgcg attgcataca atgcacacgc aaccgttgca
     1441 cctccggcca tatcgcactt catgtaatcc atggagttcg gcgtgggctt caggctcaat
     1501 ccgccggtat cgtacacaac acctttgccg accaatacat acggcttacg gtttttggct
     1561 ccgcgcggtt tgtactccat gatcgtaaaa gtcggcggct gaggactgcc ctggttgacc
     1621 gccagcagac cgcccatttt gagcgatttt atccttgcct tcttcaatac ttccacccgg
     1681 attcccgcgg aacgacctag ttttgccaac tctgaagaaa atacggtcgc gttgagatac
     1741 gactgcggtt cattgaccag ggtacgtgcc cgataaacgg catcgatcac atggttcagc
     1801 ttctcgaccg ctttacggct tacttcggtg ctttggatca ggatttccga aacggtatgg
     1861 gcttcctttt cctttccttt tgaacggtat ttcaaaaact ggtaattacc caaggccatg
     1921 ccctcggcca aggcaagggc tgtatcccca tgtccggcca catctaccag tacaacccgt
     1981 tcttttttct gacggttaaa cgcgcccaat aacgcgtctc cggcacgccg gcacgcctcc
     2041 aataaggctg ctccggtttt tttcttgtca agccggtgga ccgcgatcag acgacggtac
     2101 tggttgatca ccacattgtt gcgatccgcc ttcaactcgt gctgaacata ggcttgctcg
     2161 tcggcagaca gcagtccggt aggcaatccg cttttgtcat caagcagtaa aatgagcgaa
     2221 tccgtggccc ggtaggtgcc tgtcaggcgg a
//
LOCUS       DAOTON010000479         2250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37529_length_2250_cov_3.637358, whole genome shotgun sequence.
ACCESSION   DAOTON010000479 DAOTON010000000
VERSION     DAOTON010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37529_length_2250_cov_3.637358"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="P5292_09385"
     CDS             <1..1434
                     /locus_tag="P5292_09385"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRS39378.1"
                     /translation="LRKLLRAARSTAFGKHYGFDELLSARDFSARFQNRVPLFTYNSI
                     YKDWWHRCLAEETDVCWPGYVKYFALSSGTSESASKHIPVTKEMIKALRKASIRQILT
                     LGRYDLPAEFFEKGILWLGGSTDLKKHGGYYEGDVSGISARRVPIWFQRYYKPGKEIS
                     REKDWNIKLEEITRQAANWDIGIITGVPSWVQLLLEKVIERYKLKTIHDLWPNLRVYA
                     HGGVSFDPYRSSFDKFFAHPVHYIEMYPASEGFFAFQTEPGVRHMRMVMNNGIFFEFI
                     PFNETNFDDNGEPRPEAKAVTIDKAENGKDYALVLSSCAGAWRYLIGDVIRFTDVSQG
                     DIIITGRTKHFLSLVGEHLSVDNMNHAVEAVSRRFSVDIPEFTVCGVPHQGSFAHQWY
                     LGVQHGSLNVDEAGRHLDEELKRLNDDYAVERGSALHAPMITTIPAERFYQFMQANGR
                     MGGQNKFPRVMKAAQHDAWKNFLDASR"
     gene            1445..2062
                     /locus_tag="P5292_09390"
     CDS             1445..2062
                     /locus_tag="P5292_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRS39379.1"
                     /translation="MLEPLLSGITFGLALALMVGPVFFALIQTSLHEGFRAGLFFAFG
                     VFVSDASLIALGYLFAGQLDLLNTHRDIMGWVGGSVLIIFGVVQMTRQPKAKEVDDDR
                     RTVHGHYLVRGFLMNTLNPMVLLFWLSVIGIVSLREQYSLSDRFTFFGSVLGTVLSTD
                     LLKSYGAGKLKRLLNARVMKWINRITGLIVAGFGVEMIVRAFLQS"
     gene            complement(2098..>2250)
                     /locus_tag="P5292_09395"
     CDS             complement(2098..>2250)
                     /locus_tag="P5292_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRS39380.1"
                     /translation="NTKEEINQAFEDYHRTQFGGWPWPRYDQVHDRSRTRFARHADGR
                     EEVKEG"
BASE COUNT          479 a          623 c          635 g          513 t
ORIGIN      
        1 ctgcgcaagt tgttgcgcgc ggcacgttct acggcctttg gaaagcatta cggcttcgat
       61 gaactattgt cggcgcgcga cttcagtgca cgcttccaaa atcgggttcc gttgttcact
      121 tacaattcga tctacaagga ctggtggcac cgttgcctgg cggaagagac ggatgtttgc
      181 tggccgggtt atgtgaaata tttcgcgcta tcgtccggga cttccgaatc ggccagcaag
      241 cacattccgg tgacgaagga aatgatcaaa gcccttcgga aagccagcat ccggcagatc
      301 ctgacgctcg gacgctatga ccttccggca gagttttttg aaaaaggcat tctctggctg
      361 ggtggatcca ccgacttgaa aaagcatggc ggttattacg aaggcgacgt gagcggcatc
      421 tccgcaaggc gtgtgccgat ctggttccaa cgctattaca aaccgggaaa ggaaatctcc
      481 cgtgaaaagg actggaacat caaactggaa gagatcaccc gtcaggcggc gaattgggat
      541 atcggcatca tcaccggtgt tccgtcctgg gtacaattgc tgctggaaaa agtgatcgaa
      601 cggtacaagt tgaaaacgat ccacgacctc tggcccaacc tgcgcgttta tgcgcacggc
      661 ggggtatcct tcgatcctta ccggagttca ttcgataaat tcttcgcgca tcctgtccac
      721 tatatcgaaa tgtaccctgc ttcggaaggc ttctttgcct tccagacgga acccggtgtg
      781 cgacacatgc gcatggtgat gaacaacggg atcttcttcg agttcatccc gttcaacgag
      841 acgaatttcg atgacaacgg cgaaccgcga cccgaagcga aagcggtcac gatcgacaag
      901 gcggagaatg gaaaagacta cgcccttgtt ttgagcagct gcgccggcgc ctggcggtat
      961 ctgatcggcg acgtgatccg gttcacggac gtttcccagg gcgatatcat catcaccggc
     1021 cgcaccaaac acttcctgag tctggtgggt gagcacttgt cggtcgacaa tatgaaccac
     1081 gcggtagaag cggttagccg gcgtttcagc gtcgacattc ccgaattcac cgtatgtggc
     1141 gtcccgcatc agggcagctt tgcgcaccaa tggtacctcg gtgtacaaca cggttccctg
     1201 aacgtggatg aagccggtcg gcacctcgat gaggagttga aacgactgaa cgacgattat
     1261 gcggtagagc gtggttcggc cctgcacgct ccgatgatta ccaccattcc cgccgagcgt
     1321 ttttatcaat tcatgcaggc aaacggacgg atgggcgggc agaacaagtt cccgcgtgtc
     1381 atgaaggctg cccagcacga cgcctggaaa aacttcctcg acgcgtcgcg ttaatcttcc
     1441 ctgtatgctc gagcccctgc tgagcggtat cacatttggc ctggccctgg ccctgatggt
     1501 agggccggtc ttcttcgccc ttatccagac cagcctccac gaaggattcc gcgccggact
     1561 gttcttcgca ttcggggtct ttgtcagtga tgcctcgctg atcgctttgg gttacctgtt
     1621 cgccggacag ctcgacctgc tcaacacgca ccgcgacatc atgggatggg tgggcggttc
     1681 cgtcctgatc atcttcggcg tcgtgcagat gacccggcag ccgaaagcga aggaagtcga
     1741 tgatgatcgt cgcaccgtgc atggccatta cctggttcgc ggattcctga tgaacaccct
     1801 caacccgatg gtcctcctgt tctggttaag tgtgatcggt atcgtcagct tacgcgagca
     1861 atacagcttg tcggatcgct tcaccttttt cggaagtgtg ctgggtacgg tactgagcac
     1921 cgacctgctc aaatcctatg gcgccggaaa actgaagcga ctcctcaatg cacgggtgat
     1981 gaaatggatc aaccgcatta ccggattgat cgtggcggga ttcggagtgg aaatgatcgt
     2041 gcgggcgttc ctgcagtctt gaacagcgtg aggaataccg ctgatacatt gtaaccgtta
     2101 cccctccttc acttcctccc gtccgtcggc atgacgcgcg aatcgggtgc gggagcgatc
     2161 gtgcacctgg tcgtaccgcg gccagggcca gccgccgaat tgtgtgcggt ggtagtcctc
     2221 aaaagcctga ttgatctctt ccttcgtgtt
//
LOCUS       DAOTON010000480         2247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37614_length_2247_cov_3.197080, whole genome shotgun sequence.
ACCESSION   DAOTON010000480 DAOTON010000000
VERSION     DAOTON010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37614_length_2247_cov_3.197080"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..473)
                     /locus_tag="P5292_09400"
     CDS             complement(123..473)
                     /locus_tag="P5292_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRS39381.1"
                     /translation="MSDIHHEVISHASEDKAHSALNTKVIWKVFWILLGVTIFEVGIA
                     FTSISPTILMYIFIALTIVKSYYIVGYFMHMKFEVMPFQWSVLLPFILIVYLIFIALY
                     EGSALSMAGTSVGY"
     gene            complement(500..1312)
                     /locus_tag="P5292_09405"
     CDS             complement(500..1312)
                     /locus_tag="P5292_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRS39382.1"
                     /translation="MANHAAVAESGQSVWGGGRSPFQVSWGKMFMWFFLVSDAFTFSS
                     LLIAYGYMRHRFTEIWPKPGDVFTHFPFIHGHVPLAYVGLMTFILIMSSVTMVLAVDA
                     GHSLKKNGVIRWLWLTIIGGFMFLGSQAWEWYHFIHGSEKGSFILADGRFARTDDLEP
                     GTLIFHDHSKVEGAEAQELMSTAVRNIQGANLKENEYGRTPLFANFFFFITGFHGFHV
                     FSGVVLNIIVLALVYQNFCEKKKDYELVEKVGLYWHFVDLVWVFVFTFFYLV"
     gene            complement(1378..2028)
                     /locus_tag="P5292_09410"
     CDS             complement(1378..2028)
                     /locus_tag="P5292_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRS39383.1"
                     /translation="MTASTEQSGFDKVKAQRGLMWFGIISIVMLFAGLTSAYIVRMGD
                     GKWVQFALPNTFIISTLVILASSVPMQWAVKSAEKGNQKNLVTALWLTLLLGIGFVLV
                     QYIAWTELFSQGIALTGRIKDIKTTDIVYVPSGKETFEQAGDAGNVAGSFLYVITGLH
                     IVHLLGGMIALFIVIWRANRKKYTVEKHNGVRMCALYWHFLSGLWLYLFFFLLYVR"
     gene            complement(2042..>2247)
                     /locus_tag="P5292_09415"
     CDS             complement(2042..>2247)
                     /locus_tag="P5292_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HRS39384.1"
                     /translation="PMAFLPLAFHLTGWFTALLLAACGIYFSLQAIRLYRSLEIKDAQ
                     KLMFGSFIYLPVVQLIWMLDKLV"
BASE COUNT          586 a          550 c          638 g          473 t
ORIGIN      
        1 acctatgacg ggcagccgtt tcaagtggtt ttccccgcgc gagataatca gccaaaaggc
       61 gacggggaag gcaaggatga taagcgggac ggccagccgc ttccaacggg cagccatggc
      121 ggtcagtatc cgacggatgt acctgccata ctgagcgcgg agccttcgta gagggcaatg
      181 aagatcaggt acacgatcag gatgaacggc agcaacaccg accactggaa cggcatcacc
      241 tcgaacttca tgtgcatgaa gtagccgacg atgtagtatg acttaacgat cgtcagggcg
      301 atgaagatgt acatcaggat cgtcggtgag atcgaggtga acgcgatgcc gacttcgaag
      361 atggtcacgc caagcaggat ccagaatact ttccagatca ctttggtatt caaggcagag
      421 tgcgccttgt cttcgctggc gtgggagatg acttcgtgat ggatatcaga catggtgatg
      481 cgtatgcgga gttagacgat cagacgaggt agaagaaggt aaacacgaag acccagacca
      541 ggtcgacgaa gtgccagtag agacctactt tttcgaccag ttcatagtcc tttttctttt
      601 cgcagaaatt ctggtagacc agcgcgagca cgatgatgtt aagcaccacg ccgctgaata
      661 cgtggaaacc gtggaagccg gtgatgaaga agaagaaatt ggcgaagagg ggtgtacggc
      721 cgtattcatt ctccttcagg ttcgcgccct ggatattgcg aaccgccgtt gacatgagtt
      781 cctgggcttc ggcgccttcc actttcgaat ggtcgtggaa gatcagcgtg cccggttcca
      841 gatcatcggt acgggcaaaa cggccgtcgg cgaggatgaa agaacctttt tcagaaccgt
      901 gaatgaagtg ataccattcc catgcctgag agccaaggaa catgaagcca ccgataatgg
      961 tcagccacaa ccagcggatc acaccgttct ttttcagact gtgtccggcg tcaacggcga
     1021 gcaccatggt cacggaagac atgatcagga tgaaggtcat cagaccgacg tacgccagag
     1081 gcacgtgccc atggatgaac gggaagtgcg tgaatacgtc gcccggcttc ggccagatct
     1141 ccgtgaagcg gtgacgcata taaccatatg cgatcagaag actggaaaac gtgaaggcgt
     1201 cggatacgag gaagaaccac atgaacatct tcccccagct tacctggaaa ggtgagcgac
     1261 cgcctcccca gacggactgt ccggattcgg ctacggcagc gtgattggcc atttatttcg
     1321 ctgaattatg agttttcggt gtcgttgtac tccgggaaag ccccaaagtt gcttttttta
     1381 gcggacgtaa agtaaaaaga aaaacaggta cagccacaag cctgaaagga agtgccaata
     1441 tagtgcgcac atcctcacgc cattatgctt ttcaaccgtg tatttctttc gatttgcgcg
     1501 ccaaattaca ataaataggg cgatcatacc gcccaagagg tgaacaatat gtaggccggt
     1561 aatgacatag agaaacgaac cggctacatt accggcgtcg ccagcctgtt caaaggtttc
     1621 ctttccggaa gggacgtaaa cgatgtcggt ggtcttgatg tctttgatcc ggccggtgag
     1681 cgcgataccc tgggagaaaa gctcggtcca ggcgatgtac tgaaccaaga cgaagccgat
     1741 acccagcagg agtgtcagcc acagggcggt gaccaggttt ttttggttac ccttttcggc
     1801 cgacttgacc gcccattgca tcggcacgct gctggcaaga atgaccaggg tactgatgat
     1861 aaacgtattc ggtagagcga actgcaccca tttgccgtct cccatccgca cgatgtaagc
     1921 ggaggtcagg ccggcgaaga gcatgacgat gctgatgatg ccgaaccaca tcagtccgcg
     1981 ctgcgctttc accttatcga atccggactg ttctgtgctt gccgtcatgg tagtcatggt
     2041 atcagacgag tttatcgagc atccagatca actgcactac cggtaggtaa atgaaggatc
     2101 cgaacatgag tttctgtgcg tctttgattt cgagcgaacg atagaggcgg attgcctgca
     2161 gggagaagta gattccacag gctgccagca gtaaggcggt gaaccaaccg gtgaggtgaa
     2221 atgccaatgg cagaaaagcc atgggta
//
LOCUS       DAOTON010000481         2244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37685_length_2244_cov_3.292371, whole genome shotgun sequence.
ACCESSION   DAOTON010000481 DAOTON010000000
VERSION     DAOTON010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37685_length_2244_cov_3.292371"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..>2244
                     /locus_tag="P5292_09420"
     CDS             20..>2244
                     /locus_tag="P5292_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS39385.1"
                     /translation="MSLPVRYTDTLGFVVLLALSGYTAVAFQKFNREKELETRKLLAQ
                     KIDARQDHVAEYLLQEQLLSIPRDQQLKKLLDLQFNRAEAIKTHIATTNLTGYLSKFE
                     LSVRLFRSDGVQLDSVDTRTLDEFRRAAVDLGRPTIAPDLYYLPNESGQLAYIGFVRL
                     GSDTAATWLALTFDARFFPAGSGFPELFVSGTDRESRWPEAYSLARYNRDALVFEYGS
                     YSYAFTPGEFPTDSSDYKQVVRNGFEHLLYRPSTGTLIVVSRPAPRPIEVLTLFSWVF
                     SFIGGFLAIAWMVYRLTSGQTASTGTLTRRIQVSVFSLVVLSFAAIGTGTVWYIQRKY
                     ADDQRKSIAEQVNGLWLLINDQPSVHSPLSGTSPLPFEPTLSRVAANTNIDYNLFDER
                     GQLYYSSQPRIFDQGIVSTRMNPEAFLELQEERRTQFIHRESVGRLRYVAAYAPFTDE
                     RGNITGYLHLPYFEKQNELNQEISGFISALLNIYVLLFAIALLFTLLISARITQPLSV
                     LQEKLAGIRLGRRNEEIAYAGTDEIGALVNEYNRLVAELAISADKLARSERESAWREM
                     ARQVAHEIKNPLTPMKLSVQHLQRTVSDGTTDAAQVRRVTETLLQQIETLSNIAGALS
                     DFARMPAAQRVVLDLRNVLRPTVDLFRDSPGLRIDYSEPAQACFVLADKDQLSRVFQN
                     LLKNAVQAIPDERAGLIEVKLEAKDSGWEVSVTDNGIGIPESQRERIFVPNFTTKSGG
                     TG"
BASE COUNT          477 a          658 c          612 g          497 t
ORIGIN      
        1 gttggcggct ctggctaaga tgtcgctgcc ggttcgttat acggacacac ttggtttcgt
       61 agtactcctc gcgctgagcg gttataccgc ggttgcgttt cagaaattca accgggaaaa
      121 agaactggag acgcggaagc tgctggcaca gaagatcgat gcgcgtcagg accatgttgc
      181 ggaatacctg ttgcaggagc aactgttgtc gattccgcgc gatcagcaac tgaagaaact
      241 gcttgatctc cagttcaacc gcgccgaagc gatcaaaacc catatcgcca cgaccaacct
      301 gacgggctat ctgagcaagt tcgaactgtc ggtgcggctg tttcgatccg atggtgtgca
      361 actggattcg gtcgataccc gcaccttgga tgaattccgc cgcgcagcgg tcgatcttgg
      421 ccgcccgacc atcgctccag acctgtatta tctaccgaat gaatccggac aactcgccta
      481 catcggattt gtgcgactgg gttcggatac ggccgctacc tggttagccc tgacgttcga
      541 cgcgagattc ttccccgccg gttccggttt tccggagttg ttcgtgagtg gcacggaccg
      601 ggaaagccgt tggccggaag cgtattcgct ggctcgttat aaccgcgatg ccctggtatt
      661 cgaatatggc agttattcgt acgcgtttac tcctggcgaa ttcccgacgg attccagtga
      721 ctacaagcag gtagttcgga acggcttcga gcacctcttg taccgcccat caaccggtac
      781 actcattgtg gtcagccgcc cggcacctcg tccgatcgaa gtattgacgc tcttcagctg
      841 ggttttcagt ttcatcggcg ggtttctggc gatcgcctgg atggtctatc gattgacctc
      901 cggtcaaacc gcatcaaccg gaaccttgac ccgcaggata caggtatcgg tcttctccct
      961 cgttgttctc tccttcgcgg ccatcggtac gggcacggtc tggtacatcc aacggaagta
     1021 tgccgacgat cagcgaaaga gcatcgcgga acaggtaaac ggcttgtggt tgctgatcaa
     1081 cgatcaaccg tcggtacatt cgcccttatc gggtacatca ccgcttccgt tcgagccgac
     1141 actctcgcgc gtcgccgcga acacgaacat cgactacaac ctcttcgacg aacgcggaca
     1201 actctactat tcttcccagc cgcgcatctt cgaccaggga atcgtttcga cccggatgaa
     1261 tccggaagcg tttctcgaat tgcaggagga acgacgcacg cagttcatcc accgggaaag
     1321 tgtgggtcga ctgcggtatg tcgccgctta tgcgccgttc accgatgagc gcggcaacat
     1381 cacgggttat cttcacttac cgtatttcga aaagcaaaac gaactcaacc aggagatctc
     1441 cggatttatc tccgctttgc tcaacatcta tgtattgctc ttcgccatcg ccttgctctt
     1501 taccctgctg atctccgcgc gcatcaccca gccgctttcc gtcttgcagg aaaagcttgc
     1561 tggcattcga cttggccgcc ggaacgagga gatcgcctat gcgggaaccg acgagatcgg
     1621 cgccctggtg aacgaataca accggctggt cgccgaactg gcgatcagcg ccgacaaact
     1681 ggcacgcagc gaacgcgaat ccgcctggcg ggaaatggcg cgtcaggtag cacacgagat
     1741 caaaaacccg ttgacaccga tgaaattgtc ggtacaacac cttcagcgta cggtttcgga
     1801 cggaacgacc gacgctgcgc aagtgcgtcg cgtgacggaa accctgttgc agcagatcga
     1861 aaccctgtcg aacatcgccg gcgcgttatc cgacttcgcc cgcatgcccg cagcacagcg
     1921 cgtggttctt gatcttcgca acgtattgcg cccaaccgtt gatcttttcc gggattctcc
     1981 cggcttgcgc atcgactatt ccgaacccgc gcaggcatgt tttgtcctgg ccgacaagga
     2041 ccaactatcg cgggtgtttc agaacctgct gaagaatgct gttcaggcaa ttccggacga
     2101 acgcgcggga ttgatcgaag tgaaactgga agcgaaggat tccggctggg aagtatccgt
     2161 gacggacaac ggtatcggca ttccggaatc gcagcgcgaa cggatcttcg tgccgaattt
     2221 tacgaccaag tcgggcggta ccgg
//
LOCUS       DAOTON010000482         2244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37692_length_2244_cov_2.505710, whole genome shotgun sequence.
ACCESSION   DAOTON010000482 DAOTON010000000
VERSION     DAOTON010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37692_length_2244_cov_2.505710"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="P5292_09425"
     CDS             complement(<1..1298)
                     /locus_tag="P5292_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRS39386.1"
                     /translation="MSKIKFGTDGWRAIIAKDFTVANVARVAEATAAWLKKQKKQPHI
                     VLGHDCRFAGELFAETVAKVLCERGVKVSLARGFVSTPMISLGAVKLKADLGVILTAS
                     HNPPSYNGFKLKGSFGGPLSPSDVQQIEDIIPDHCSLDLDRIVLAGFEKTGMLEYVNL
                     EDLYIRHVEANFDLEAIRRSGMKLAYDAMFGAGQNVMRRLFPDITLLHCDNNPGFHGQ
                     APEPIHKNLRDLHQLLTSTKGYSCAVVTDGDADRIGMYDGEGNFIDSHHIILLLIHYL
                     VKYKGMTGKVCTAFSTTPKVEKLCKHYGLTLETVKIGFKYICEIMVREDVLIGGEESG
                     GIAIKGHIPERDGIWMGLVIWEFMAKSGKTLPELIREVYDIVGPFSFERNDLHLDQAV
                     KDRIVANTAAGAYSAFGPYKVARIDDLDGYKYFFDNENRE"
     gene            complement(1327..>2244)
                     /locus_tag="P5292_09430"
     CDS             complement(1327..>2244)
                     /locus_tag="P5292_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39387.1"
                     /translation="GKVTLWDGPKKNFAITPEALARVERSSGTLIRDLPQLFIYEYWN
                     LDQKKGRLRTLGYFLSSRTTSGEEVSFGYVDHAPLDSLFRASLLKLNANGDCKLDFGT
                     VLLNKYYLYTILQFGNKQVKDVVDALRLRESVRQFVTGKTGAPEHTCKRITWMVEDSM
                     TVRDEPTAAAARLLGSIQQYLNENREVYFNLGGDALSDFTVEKPLRLSAIEVEEEWHR
                     NGEIIESRLKAVRFWVDGKPLRSTSLEDLARMELVVGFRQFLDLLNEKEFYFRILQLN
                     DETIAPDRSSGLKKALQTWEWNRLSEFVP"
BASE COUNT          516 a          617 c          554 g          557 t
ORIGIN      
        1 cattcccggt tttcgttatc aaagaagtat ttgtaaccgt ccaggtcgtc tattctcgcg
       61 accttgtagg ggccgaatgc cgaataggca ccagccgcgg tattcgcaac gatccggtct
      121 ttcacagcct gatcaaggtg caggtcgttt cgctcaaaag aaaacggacc gacgatgtcg
      181 tacacttccc ggataagttc cggcagggtt tttccgctct tcgccatgaa ttcccagatc
      241 accaatccca tccagattcc atcgcgctcc gggatatgac ctttgatggc gattccaccg
      301 ctctcttccc ctccgatcag tacatcttcc cgcaccatga tctcacagat gtacttgaat
      361 ccgatcttga ccgtctcaag cgtcagaccg taatgcttgc atagtttctc caccttgggt
      421 gtggtactga aagccgtgca gaccttaccc gtcattccct tgtattttac cagatagtgg
      481 atcagcaaga ggatgatgtg gtgcgagtcg atgaagtttc cttcgccatc gtacatgccg
      541 atccggtcgg cgtcgccatc ggttacgaca gcacaggaat atcctttggt gctggtcagt
      601 agttggtgga gatcgcgaag gttcttatgg atcggttccg gcgcctgccc gtggaatccg
      661 gggttattgt cgcaatgcaa taaggtgatg tcggggaata gtcggcgcat tacattctga
      721 cctgcgccaa acatggcgtc atacgccaat ttcattcctg aacgacggat agcttccagg
      781 tcaaaattcg cttcgacgtg tcgtatgtac aaatcttcca ggttgacata ttccagcatc
      841 ccggtctttt caaatcctgc caaaacgatc cggtcaaggt caagcgagca gtggtccggg
      901 atgatatctt caatctgttg aacatccgac ggactgagcg ggccgccgaa cgagcccttc
      961 agtttgaaac cattgtagga aggcggattg tgagaagccg tcaggatgac tcccagatcg
     1021 gccttcagtt tcaccgcgcc gagggaaatc atcggagtcg aaacgaatcc cctggccagg
     1081 gaaaccttga cgccgcgttc acagagcacc ttggcgactg tctcggcaaa cagctcgccg
     1141 gcaaagcgac agtcgtgacc taatacaata tgaggctgtt tcttttgctt cttcaaccag
     1201 gctgccgtag cctctgcgac gcgtgccaca ttggcaacgg tgaaatcctt cgcgatgatg
     1261 gcacgccagc catcggttcc aaacttgatc ttgctcattc gatatcttct gtgacgccct
     1321 aagtcttcag ggaacgaact cggaaagccg gttccattcc caggtctgaa gggctttttt
     1381 cagcccactc gaacgatccg gcgcaatggt ttcgtcgttc aattgcagga tgcgaaaata
     1441 aaactctttt tcgttcaaaa ggtctaaaaa ctgcctgaaa ccgactacta attccatcct
     1501 cgcaaggtct tccagcgagg tggatctcaa tggttttccg tccacccaaa agcggacggc
     1561 cttaagccgg gattcgatga tttcgccgtt ccggtgccac tcctcttcga cttctatcgc
     1621 ggagagccgg agcggtttct ctacagtaaa atcgcttaaa gcatcaccgc ccaggttgaa
     1681 atacacttcc cgattttcat tcaggtattg ttgtatagaa cccagcagcc ttgccgctgc
     1741 ggccgttggt tcatcccgta ccgtcatgga atcttccacc atccaggtga tgcgcttgca
     1801 ggtatgctcc ggagcaccgg tctttccggt aacgaactga cggaccgatt cgcgcaaccg
     1861 gagggcatcg acaacatctt tcacctgctt gttgccaaat tgaaggatgg tgtacaggta
     1921 atacttgttc agcaagacgg ttccaaagtc aagtttgcaa tcgccattgg cgttgagttt
     1981 gagcagcgag gcgcggaata aactgtccag gggagcatga tcgacataac caaaagaaac
     2041 ttcttccccg gacgttgtac gggaagagag aaaatatccc aacgtccgga gccgcccttt
     2101 tttctggtca aggttccagt attcgtagat aaacagttgg ggcaggtcac ggatcaaggt
     2161 acccgaacta cgctccaccc gggcgagcgc ttccggtgtg atggcaaaat tcttctttgg
     2221 accatcccac aacgtcacct ttcc
//
LOCUS       DAOTON010000483         2242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37738_length_2242_cov_3.102423, whole genome shotgun sequence.
ACCESSION   DAOTON010000483 DAOTON010000000
VERSION     DAOTON010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37738_length_2242_cov_3.102423"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="P5292_09435"
     CDS             complement(<1..243)
                     /locus_tag="P5292_09435"
                     /inference="COORDINATES: protein motif:HMM:NF023504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HRS39388.1"
                     /translation="MINMMYLVLTALLALNVTKEVINAFVTINESVQLSKDNLDKKNQ
                     NTYAAFAQAMSVDANKYKDVNDRANAVKKSAGDLVKF"
     gene            complement(321..1082)
                     /gene="gldL"
                     /locus_tag="P5292_09440"
     CDS             complement(321..1082)
                     /gene="gldL"
                     /locus_tag="P5292_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008203296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HRS39389.1"
                     /translation="MAKLYGWGASIVIVGALFKIQHWPYSGFFLVLGLGTEAVIFFFS
                     AFEPPHEDVDWSLVYPELAGMGDDDHGKREKRKKADPVAQKLDALMADAKIGPELIES
                     LGTGLKSLGDNAGKLADLSDASVATNDYVKNVAKAATEVGNMSNSYTKAAAAIDGLSS
                     STEEVKVYKDQVVTAGKNLAALNAVYELQLQDSNEHLKQTSRFYEGINELMGNLNASL
                     DDTRKYRDEVAHLAQNLSKLNTVYGNMLSAMTIKS"
     gene            complement(1241..>2242)
                     /locus_tag="P5292_09445"
     CDS             complement(1241..>2242)
                     /locus_tag="P5292_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRS39390.1"
                     /translation="RQFVYWVRDSLAHRLIGGEHLIEEGEYGERINWKAKIKWDDEQN
                     EETLAELFLPETERFYRRKEIDTRKLNFEYYWIDLKAAAQRENREQGMRDRSVFIKKD
                     VINVYPDTLAWIHDYAYSYHEPLTKMYFWHPAYDDYPVVGVNWKQARAFCIWRTQLLN
                     SYLADEGGAFVQDYRLPTESEWEYAARGGLELSPYPWGGPYIRNSRGCFLGNFKPMRG
                     SYQDDGGVYTVKATSYWPNDYGLYCMAGNVSEWTSNAYDESAYSFTHDISTDYEYDAK
                     DSDPPALKRKVIRGGSWKDVMYFLQTGTRSYEYQDTSKCYVGFRCVMSFLGRDKSDY"
BASE COUNT          446 a          588 c          656 g          552 t
ORIGIN      
        1 aaacttcacc agatcgccgg ccgacttctt gaccgcattg gcccggtcgt tgacgtcctt
       61 gtacttgttg gcgtcaaccg acatggcctg cgcgaaagcc gcgtaggtgt tctggttctt
      121 tttatcaaga ttgtctttcg acaattgcac gctttcattg atcgtgacga aggcgttgat
      181 gacctctttc gtcacgttca gggcaagaag ggcggtcaac acgagataca tcatgttgat
      241 catcttttgc ctcggggtta gttttccacc tgccatctca gctgaatgtg tttaagttgg
      301 ttagacgttg aataacacaa tcagctcttg atggtcattg cggaaagcat gttgccgtaa
      361 accgtgttga gtttggacag gttctgggca aggtgcgcca cttcgtcgcg gtatttgcgg
      421 gtatcgtcga gcgaggcgtt gaggttgccc atgagctcgt tgatgccttc gtagaagcgg
      481 ctggtctgct tgaggtgttc gttgctgtcc tgcagttgca gttcgtacac ggcgttgagc
      541 gcggccaggt tcttaccggc ggtgaccacc tgatccttgt acactttcac ttcttccgta
      601 gaagaggaaa ggccgtcgat cgcggcagcg gccttggtgt acgaattcga catattgccg
      661 acttcggtag cagccttggc aacgttcttc acatagtcgt tggtagcgac ggaagcgtcg
      721 ctgaggtcgg ccagtttgcc ggcgttgtcg cccagcgact tcaggccggt acccaggctt
      781 tcgatcagtt ccggaccgat cttggcgtcg gccatcaggg catcgagttt ctgtgcgacc
      841 ggatccgcct tcttgcgctt ctcccgcttg ccatggtcat catcgcccat accggcgagt
      901 tcaggatata ccagcgacca atccacgtct tcgtggggag gttcgaaggc ggagaagaag
      961 aagatcacgg cttctgtgcc gagacctaat acgaggaaaa atccggagta cggccagtgc
     1021 tggatcttga acagcgcgcc cacgataacg atggatgcgc cccatccata gagtttggcc
     1081 atgaagtttt tgaacttctt accttgagtt aattcggcaa gtcccatgtt gtacggtttg
     1141 gtttgttttg gttagacaat ggtggtgtaa ttcgtagtaa ggaggggaac ccgatggctg
     1201 acgcagtctt gaccgggttc aggaaaggcg ataagccgga tcaataatcc gacttatcac
     1261 gacccaggaa gctcatcacg cagcggaagc cgacgtagca cttggaggta tcctggtatt
     1321 cgtaggagcg cgttccggtc tggaggaagt acatgacatc cttccaggag ccgccacgga
     1381 tgactttgcg cttgagcgct ggcggatcgg aatctttcgc gtcgtattcg taatcggtac
     1441 tgatatcgtg ggtgaagctg tacgccgact cgtcgtaggc gttgctcgtc cactcggaga
     1501 cgttaccggc catgcaatac aggccgtaat cgttcggcca gtacgaagtg gctttcacgg
     1561 tgtacacacc gccgtcgtcc tgataggaac cgcgcatcgg cttgaagtta cccaggaaac
     1621 agccgcggct gttgcggata tacggaccgc cccaggggta cggggagagt tccaggccgc
     1681 cgcgcgcggc gtattcccat tccgattcgg tggggagacg gtagtcctgc acgaaagctc
     1741 cgccttcgtc ggccaggtag gagttcaaca gttgggtacg ccagatgcaa aatgcacgtg
     1801 cctgcttcca gttgacgcca accacaggat aatcgtcgta cgccggatgc cagaagtaca
     1861 tcttggtcag cggctcatgg tacgaataag cgtaatcgtg gatccaggcc agggtgtcgg
     1921 gatacacgtt gatgacgtcc ttcttgataa agaccgaacg gtcgcgcata ccctgctcgc
     1981 ggttctcgcg ctgggcagcg gccttcaggt cgatccagta gtattcgaaa ttgagtttac
     2041 gggtatcgat ctccttgcgg cgatagaagc gctccgtttc gggcaggaac agctcggcaa
     2101 gggtctcttc gttctgctcg tcgtcccact tgatcttggc tttccagttg atacgttcgc
     2161 cgtactcgcc ctcttcgatc aggtgttcgc cgccgatcag acggtgtgcc agggagtcgc
     2221 gcacccaata cacgaactga cg
//
LOCUS       DAOTON010000484         2238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37831_length_2238_cov_3.287678, whole genome shotgun sequence.
ACCESSION   DAOTON010000484 DAOTON010000000
VERSION     DAOTON010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37831_length_2238_cov_3.287678"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="P5292_09450"
     CDS             complement(<1..916)
                     /locus_tag="P5292_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39391.1"
                     /translation="MNRPPFLLLALYAFLAAVLFPYLCWYVNNPDTFQYLMITRHYLE
                     GDFREAINGYWSPLLSWLMLPFTGQDFGDIPALKTLQLVIGAGVLWTWTSVLRRSVMP
                     EPYAEALSYAALPFVLAYAFLSPTADLLFLWLTLALFRFLAKGSALTDEQNAKRFALL
                     GALAYYAKAFGLPLFLFLLVSEWWQVRKLAVGRRLLLRALGIFLLLIAPWVTAISLKY
                     GHFTISEAAAFNRSPGVAPMPGQVMQLPVLSNELTKPSGPHAICAWEEPMQLFWTGRV
                     SEPTCVVPGFLQVLERNLLTIWYFDFQRT"
     gene            1150..>2238
                     /locus_tag="P5292_09455"
     CDS             1150..>2238
                     /locus_tag="P5292_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRS39392.1"
                     /translation="MKDTTTLQDYGIAEALKALGLKDINPGAGTGTRWSESDGEIISS
                     YSPADGTLIGKVKQATADDYEKTLRTAEEAFKVWRNMPAPKRGEIVRQIGDQLRKYKD
                     PLGKLVSYEMGKSLQEGLGEVQEMIDICDFAVGLSRQLYGLTMHSERPKHRMYEQWHP
                     LGIVGIISAFNFPVAVWSWNAAIAWVCGDVCIWKPSSKVPLCAVACQNIIASVLKANN
                     VPEGVSCVLVGNHVGDRMNNDKRVPLVSFTGSTRVGRIVSSAVAQRFGKSILELGGNN
                     AIIVSEHSDLNMVLVGTVFGAVGTAGQRCTSTRRLIVHESVYDKTVSVLQKAYAQLRI
                     GNPLDANNHVGPLIDKGAVDAYLKAIEKA"
BASE COUNT          543 a          641 c          624 g          430 t
ORIGIN      
        1 tagttcgttg gaaatcgaaa taccaaatgg tgagcaggtt gcgttccagc acctgaagga
       61 atccggggac cacacacgtc ggttccgata cgcgtccggt ccagaacaat tgcatcggtt
      121 cttcccaggc gcagatcgca tgcggaccgg atggtttcgt cagttcgttg gagagcaccg
      181 gaagctgcat gacctgtccc ggcatgggtg cgacacccgg actacggttg aacgcggccg
      241 cctcgctgat cgtaaagtgc ccatacttga gactgatggc tgttacccag ggcgcgatga
      301 gcaagaggaa aatccccaaa gcacgcagca ggagtcgtct cccgacagct agcttgcgca
      361 cctgccacca ttccgatacc agcaggaaca aaaacaaggg caacccgaat gctttcgcat
      421 aataggcaag cgcgccgagc aaggcgaatc gtttcgcgtt ctgttcatcg gttaaggccg
      481 aacctttggc cagaaagcga aacaacgcga gtgtaagcca gagaaacaac aggtccgcgg
      541 tcggactcag gaaagcatac gccaggacaa atggaagcgc cgcatacgac aaagcctccg
      601 cataaggctc cggcataacc gaacgacgca acacgctggt ccaggtccac agcaccccgg
      661 ctccgatgac caattgcagt gtttttaatg ccgggatatc tccgaaatcc tgcccggtaa
      721 acggcagcat cagccagctc agcaatgggc tccaataacc attgatcgcc tcccggaagt
      781 caccttccag gtagtgccgg gtaatcatca ggtactggaa ggtgtcggga ttattgacgt
      841 accaacacag gtaagggaac aggaccgccg ccaggaaagc atacagggcg agtaatagaa
      901 atggcggccg gttcataagg gggcaaaata gcgaatctga aaaggaaaat cggatgcgtg
      961 ttccgggttt cgcgtttcga gtttcgggtt tcgggttaat ggggcgaaag agaattagtt
     1021 tttagttacc agctaccctt ccgaattcct cgtctaaaac ctgtttttct ttcaactttc
     1081 caccgttcaa ctttcaactt aaagcgaaag tcatttcaat gcctcccgct attgccgtac
     1141 ctttgcggca tgaaagacac taccacgctc caggactacg gcattgccga agccctgaag
     1201 gcacttggcc tgaaagacat caacccgggc gccggaacgg gcacccgctg gtccgaatcg
     1261 gacggtgaga tcatctcttc atattccccg gccgacggca cgctcatcgg aaaggtgaag
     1321 caagcgacgg cggacgatta cgaaaagacc ctgcgcacag cggaggaagc tttcaaggtg
     1381 tggcgcaaca tgcccgcacc gaaacgcggg gagatcgtcc gacagatcgg cgaccagttg
     1441 cggaaataca aggacccgct gggcaagctc gtttcgtacg agatgggcaa gagcctgcag
     1501 gaaggactgg gggaagtaca ggagatgatc gacatctgcg atttcgcggt cggtctctcg
     1561 cgccagctct acggcctgac catgcacagc gaacgtccga agcaccgcat gtacgagcaa
     1621 tggcatccgc ttggcatcgt cggcatcatt tccgctttca acttcccggt tgccgtatgg
     1681 agttggaacg cggccattgc ctgggtatgc ggtgacgtgt gcatctggaa gccgagttcg
     1741 aaagttccgc tttgcgcggt tgcttgtcag aacatcatcg cttctgtact gaaagccaac
     1801 aacgttccgg aaggcgtcag ttgcgtactg gtgggcaatc atgtgggcga ccgcatgaac
     1861 aacgacaagc gtgtaccgct cgtttcgttc accggctcca cgcgggttgg tcgcatcgtt
     1921 tcctccgccg tcgcgcaacg cttcggcaag tcgatcctcg aattgggtgg caacaacgcc
     1981 atcatcgttt ccgaacactc cgacctgaac atggtactgg tcggtaccgt cttcggcgcg
     2041 gtcggcacgg ccggtcagcg ctgcacctcc acccgccgcc tgatcgtgca cgaaagcgta
     2101 tacgacaaga ccgtcagcgt actccagaaa gcctacgcgc aactgcgcat cggcaatccg
     2161 ctcgacgcga acaaccacgt cggtccgctc attgacaaag gcgcggtcga cgcgtacctc
     2221 aaagccatcg aaaaagcg
//
LOCUS       DAOTON010000485         2235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_37910_length_2235_cov_3.074771, whole genome shotgun sequence.
ACCESSION   DAOTON010000485 DAOTON010000000
VERSION     DAOTON010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37910_length_2235_cov_3.074771"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="P5292_09460"
     CDS             <1..739
                     /locus_tag="P5292_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39393.1"
                     /translation="VISHGAKPDNTGGGSYFTGTQYLIFDALKPFLLKSALFYANGDG
                     PRTIFLSDATGNTIQSVTVNIQDGMSREDLGIEVPVGTNLRLGVSGTPNLYRNNAGVA
                     YPYILQDTLSITGSSAGASYYYFLYDWEIEVGGITCESQPALSQVFVETCTGIGGGLE
                     EKFKLTISPNPSDGVFQIRVNGRSTLGEMGMNVFDLAGRLLFSRVVQSGSNEWTHTLD
                     LVGLPAGTYLFTLETNEGRLHRRVIVR"
     gene            832..>2235
                     /locus_tag="P5292_09465"
     CDS             832..>2235
                     /locus_tag="P5292_09465"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRS39394.1"
                     /translation="MGLHIAHWKIAGKTLAPLEFNEVLYTLHLGILTAGFIFMGLAMV
                     STLLFGRFFCSWMCHMLALQDASAWILRKLKIKPRHIRSRTFYWIPVGAVIYLFLLPQ
                     LERMFMGQPAVSIHIAGASSNWASFVTTDFWRNLPSIGITLLTFLVCGGFVVYVLGSR
                     TFCQYACPYGMLFSVADRVAPGKILLKGNCTSCGICTSVCDSHIQVHREVNAYGRVMD
                     TNCLKDLDCVQVCPEGALEFGFGQPSGFFTRVASKSGKRKFDFSWQEDAVLLIAAIVF
                     IVIYRGLYDAVPFLLAITLGILLAFGLVISLRVLKRQYVRLGPILLSRPNHRTARGRW
                     FLVLYGGLILFSIHSAIVHWHTWRGEQAYNHLAGESRKNGWEAGKYAGTYQTAVEHLS
                     KAEQWGLYRPASLSRELASLALLRKDTAAAIGYFTSLLQEIPEDLEAGLRFARLLKAK
                     GEIDNAIRILEKVPSGVI"
BASE COUNT          501 a          484 c          615 g          635 t
ORIGIN      
        1 agtaatttcc catggagcaa aaccggacaa taccggtggc ggtagttact ttaccggaac
       61 gcagtacttg atttttgacg cgttgaaacc tttcctgttg aaatccgcgc ttttctatgc
      121 gaacggggac ggaccccgta cgatctttct atcagatgca accggaaata ccatccagtc
      181 ggtcactgta aacatacagg atggcatgtc gagagaggac ctgggaatag aggtgccggt
      241 cggcaccaat cttcggctgg gtgtttccgg cacaccgaac ctctatcgta acaacgcggg
      301 cgttgcttat ccgtatatcc tgcaggatac cttatctatc accggttcca gtgccggggc
      361 ctcctattac tacttccttt atgattggga aatagaggtt ggaggaatta cgtgtgaaag
      421 tcaaccagcc ttatcacaag tatttgttga aacctgtacc ggtattggag gtgggttgga
      481 agagaaattc aagctgacga tctcccccaa tccttccgat ggggtatttc aaatcagggt
      541 gaatgggcgt tcgacattag gcgaaatggg tatgaacgta ttcgatttgg ccggaaggtt
      601 gctcttttcc cgtgtggttc agtctggtag caatgaatgg actcatacac tcgatctcgt
      661 tgggttgcct gctggaacat acctgtttac actggaaacg aacgagggca ggttgcacag
      721 acgggtgatc gttcgatgat cagccgtaaa tcctgaattc agatgccgct aaagcagggt
      781 acggattatg ccagatggcg ggtgatcagt ctggcttccg tatatctgct gatgggcttg
      841 cacattgcgc attggaagat cgcaggtaag actttagcgc cgcttgaatt caatgaagtg
      901 ttatacacgc ttcatcttgg aattctgacg gcaggcttca tttttatggg ccttgccatg
      961 gtttccacct tgctcttcgg gcgatttttt tgctcctgga tgtgccatat gcttgctctt
     1021 caggatgcaa gcgcctggat actgagaaag cttaagatca agcctcggca tattcgttca
     1081 cggacatttt attggattcc ggttggggct gtgatttact tattcttgtt gccgcagctg
     1141 gaacggatgt tcatggggca acccgctgtt tcaattcata ttgcgggtgc ctcaagcaat
     1201 tgggcatcat ttgttacgac agatttctgg agaaatcttc cgtccattgg cattacgctt
     1261 cttacgttcc tggtttgtgg aggatttgtg gtttatgttc tggggagccg gacgttttgt
     1321 caatatgcct gtccgtatgg aatgctgttt tcagtggcgg atcgtgtagc acccggtaag
     1381 atcctgttaa aaggtaattg tacgagctgc ggcatttgca cttcggtctg cgattcacac
     1441 atccaggttc atcgtgaggt gaatgcatat ggacgtgtca tggatacaaa ctgcctgaaa
     1501 gatctggatt gtgttcaggt atgtcctgaa ggagcgttgg aattcggttt tggtcagcct
     1561 tccgggtttt ttacccgcgt tgcatcgaag tcgggcaaaa gaaaatttga tttttcctgg
     1621 caggaggatg ctgtgctatt gatcgctgca atcgtattta tagtcattta caggggttta
     1681 tacgacgcgg ttccttttct attggccatt actcttggaa tattactggc attcggactg
     1741 gtaatttcgt tacgggtctt gaagcgacag tacgtacggt tgggcccgat cttgttgtcg
     1801 cgtcccaatc atcggaccgc acgcggacga tggttcctgg tcctctatgg agggttaatt
     1861 cttttttcaa tccatagcgc catcgtgcat tggcatacct ggaggggtga gcaggcctac
     1921 aatcacttgg cgggagaaag tagaaagaac ggttgggagg ctggaaagta tgcgggaaca
     1981 taccagacgg cggtcgaaca cttatcgaag gctgagcagt gggggctcta tcgtcctgca
     2041 agtttgtcgc gggagttggc ttcgctggcg ctacttcgga aggacaccgc agcagctatc
     2101 ggatatttca ccagcctgct gcaggaaatt ccggaagatc tggaagccgg acttcggttc
     2161 gctcggttac tcaaagcgaa gggagagatt gataatgcga tacggatttt agaaaaagtc
     2221 ccatcaggtg taatc
//
LOCUS       DAOTON010000486         2228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_38057_length_2228_cov_3.240221, whole genome shotgun sequence.
ACCESSION   DAOTON010000486 DAOTON010000000
VERSION     DAOTON010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38057_length_2228_cov_3.240221"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..2149)
                     /locus_tag="P5292_09470"
     CDS             complement(122..2149)
                     /locus_tag="P5292_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS39395.1"
                     /translation="MSHHPFIFFFLLVLGLFRTADGAQVQYASVMQEYSSSVGNKQFG
                     PLQVLGKPDAMPQGGKSPCAWMSRLANSGKEYLEVSFDSLQVVRQVAVFENLNPGGVV
                     AVSLIDEQGKTNRIYKGPAAATSEKSRVFRVFNSDDTRQYKRVRVDFDFSKFGGQKQI
                     DAIAISDSEEPIQAEIHELKDLAWASGPENLGRKVNSAFSDVNPVISPDGRTLYFTRK
                     DHPDNINGSDENDDIWYSEIDEKGNWGPATNMDEPLNNASNNFVESVTPDGNTLLLGN
                     HYFRTSEEADGQELNGVSFAYRALDGWSFPRNIRIENYRNYDQYVNYFLSNDGRALLM
                     GIEHDDTEGAADLYVSFLQADSSWSAPVNLGHVINSAGSEGTPFLAADGVSLYYTSDG
                     FAGYGSSDMYMSKRLDNSWKNWSEPVNLGGRLNTAGWDAYFTLPASGEYAYFVSSENS
                     LGATDVFRIRLPKQMKPEPVVLVSGRVLDAKSNAPLEATIRYERLPEGASAGLARSEP
                     KDGQYRIVLPRGARFGFRAEAAGYFPVSAFINLDSLLDYQELSRDLSLVPMEVGQVVR
                     LNNIFFDFGKATLQPESEPELRRVVEFMRANSGMRIEVDGHTDDVGADAANLQLSKER
                     AASVIRFLIENGVEASRLLSEGYGETRPITGNDSEEGRSLNRRVEFKILKL"
BASE COUNT          540 a          640 c          519 g          529 t
ORIGIN      
        1 ccatgaaaaa gtgagggccg ttccaggata atcggcttgc gcctgactga cgctaccgcg
       61 tttctttcga cagacacctt tagcgtagca ttgctcaatc tatccatgtt ctttttcgct
      121 gtcacaattt caatatcttg aattccaccc gacgattcag actgcgtcct tcctcgctgt
      181 catttccggt aataggacgg gtttcaccat atccctccga aagcaagcgg gacgcctcta
      241 ctccgttttc aatcaggaac ctgataacag aagcggctct ttccttcgat agttgaaggt
      301 tggccgcatc agcccccaca tcatcggtat gaccatcgac ttcgatccgc atgcccgaat
      361 ttgcgcgcat gaactcgaca actcgcctga gttcgggttc ggattccggt tgcaaggtgg
      421 cctttccgaa atcaaagaaa atgttgttca accgtaccac ctgaccaacc tccatgggca
      481 caagcgacaa gtcgcgcgaa agctcctggt aatccaacaa tgaatcaagg ttaataaagg
      541 cgctgactgg aaaataaccc gccgcctcgg ctctgaatcc aaagcgcgcg cctcggggca
      601 gcacgatccg gtattgacca tctttcggtt cactccgcgc gagaccggcg gaagcgcctt
      661 ccggcagccg ctcataccga atggtcgcct caaggggcgc atttgacttt gcgtccaata
      721 ctcttcccga gaccagaaca accggttccg gcttcatttg cttgggaaga cggatccgga
      781 aaacatccgt tgcacccaat gaattttccg aactgacgaa ataggcatac tcgccggatg
      841 ccggaagtgt gaagtaggca tcccagccgg cagtattcag tcgccctcca agattcaccg
      901 gttccgacca attcttccag gaattgtcga gtcgcttcga catgtacata tcgctacttc
      961 cgtagcccgc aaatccatca ctggtatagt agagcgaaac gccgtcggcc gccagaaacg
     1021 gagtcccttc cgagccagcc gaattgataa cgtgacccag attcaccgga gccgaccacg
     1081 aactgtcggc ttgcaggaaa ctaacataca gatcggccgc gccttccgta tcatcgtgtt
     1141 caatgcccat caacaatgcc cgaccgtcat tggacaaaaa ataattgaca tactggtcgt
     1201 agttccgata attttcgatc cggatattcc tgggaaacga ccagccatcc agggcccgat
     1261 aagcgaacga aacgccattc aattcctgtc catccgcttc ttcggaagtc cggaaatagt
     1321 gattacccaa caagagcgta tttccgtcgg gcgtaactga ttccacaaaa ttattggaag
     1381 cgttattcaa cggttcatcc atattcgttg ccgggcccca attccccttt tcatcgatct
     1441 cggaatacca gatatcatca ttctcgtcgg agccgttgat gttgtccggg tgatcctttc
     1501 gtgtgaagta cagtgtccgg ccatccggtg agatcacggg attcacatcg gaaaaagctg
     1561 aattaacctt tctccccagg ttctcagggc cgcttgccca tgccaggtct ttcaactcat
     1621 ggatctcggc ttggatcggt tcctccgagt cggagatcgc gatcgcatca atctgcttct
     1681 gtcctccgaa ttttgaaaaa tcgaagtcga cgcggacacg cttatattgc ctggtgtcat
     1741 ctgaattaaa gacgcgaaaa acgcgggact tctcactcgt tgcagccgcc ggtcctttgt
     1801 agatgcggtt cgtctttccc tgttcgtcga tgagggacac tgctacaacg ccgccaggat
     1861 tcaaattctc gaatacggcg acctgacgca ccacttgcag ggaatcaaag gaaacttcca
     1921 gatactcctt tcccgaattc gccagccttg acatccaggc gcaggggctc ttccctccct
     1981 ggggcatggc atcgggtttt cccaacacct ggagggggcc gaactgcttg tttcccacag
     2041 aagaggaata ctcctgcatg accgatgcat actgcacctg ggcgccatct gccgttctaa
     2101 acaagcccag aacaagaaga aaaaagaaga tgaatggatg gtggctcatg atcaaaaaat
     2161 gactcccggg aatgaggcca ttcccgggag aatcctctta cttgttgact ttcaattttt
     2221 ggacatct
//
LOCUS       DAOTON010000487         2227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_38085_length_2227_cov_2.995856, whole genome shotgun sequence.
ACCESSION   DAOTON010000487 DAOTON010000000
VERSION     DAOTON010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38085_length_2227_cov_2.995856"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="P5292_09475"
     CDS             <1..1133
                     /locus_tag="P5292_09475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS39396.1"
                     /translation="YMIRTDVATIGTGLLVLLISAGTVHLLFYWLVYRRFAFLKVAAS
                     GGETKPVSVIICARNESKNLQRFLPAVLDQAYPEYEVIVVNDCSWDDSGEYLEAAEKE
                     YPRLRVVNIQEQEKYKHGKKFAITLGIKAAKHDRLLFTDADCRPASRNWLTNMQYNFR
                     DGREIVLGYGAYERMPGFLNKLIRFDTAFIAMQYFGFALGGNAYMGTGRNLAYRKDLF
                     FRNKGFARHNHLPSGDDDLFVNENATSANTAIEVQPDSFTYSLPKERFADWFNQKVRH
                     TTTSKYYKGGHRFQLFLAGICPMLVWLSLIALLVSGQRTKEALVAAGVVWALKLPIFA
                     YSASKLREKDLIWLYPLLELLYSFLLPIIYLAQLLTKKRAWK"
     gene            1124..1729
                     /locus_tag="P5292_09480"
     CDS             1124..1729
                     /locus_tag="P5292_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRS39397.1"
                     /translation="MEVNPNLSEKAQVDFKLVQQAVKGDQKAYAELMSRYKDSIYFML
                     LKMVNNRDDAEDLTIEAFGKAFKNLHQYTPDFAFSTWLFKIATNNCIDFIRRKRKYTF
                     SIDKSMENDSGQEMQFEIKSPMLDPEEKMIKKQKAALMRDVVEKLKPRYKRLVELRYF
                     QERSYEEIADELQLPLGTVKAQLFRAREFLYQILKNQEEQI"
     gene            1873..>2227
                     /locus_tag="P5292_09485"
     CDS             1873..>2227
                     /locus_tag="P5292_09485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS39398.1"
                     /translation="MLLLERYFPELTQQQRDRFAALRELYGEWNAKINVVSRKDMENL
                     YERHVLHSLGIAKVISFAAGTRVIDVGTGGGFPGIPLAILFPDSQFHLIDSIGKKIKV
                     VEGVAEALGLTNVSAQ"
BASE COUNT          498 a          585 c          647 g          497 t
ORIGIN      
        1 gttatatgat ccggacggac gtcgcgacga taggtacagg cttgttggtg ttgttgatca
       61 gcgcggggac ggttcacctg ctcttttact ggctggtgta ccggcgattc gcgttcctga
      121 aagtcgccgc atccggtggt gagacgaagc ccgtgtcggt catcatctgt gcccgcaacg
      181 agtcgaagaa tctgcagcga ttcttgccag ccgttcttga tcaggcctat ccggaatacg
      241 aagtgattgt ggtgaacgat tgttcctggg acgatagcgg tgagtatctg gaagctgcag
      301 agaaggaata tccccggctg cgggtcgtga atatccagga acaggagaag tacaagcacg
      361 gcaagaagtt cgcgatcacg ttgggtatca aagctgcgaa gcatgaccgg ctgctgttca
      421 ccgatgcgga ttgtcgtccg gccagccgga actggctcac gaacatgcag tacaatttcc
      481 gcgacggcag ggagatcgtg ctgggctatg gcgcctacga gcgcatgccc ggctttctca
      541 acaagctgat ccgattcgat acggccttca tcgcgatgca gtatttcggc ttcgcgctgg
      601 gcgggaacgc ttatatggga accggtcgta acctggctta ccggaaggac ctgttctttc
      661 gcaacaaggg tttcgcgcgg cataatcacc tgccgtcggg cgacgacgac ctttttgtga
      721 atgaaaacgc cacgtcagcg aatacggcca tagaagtaca gccggattcg ttcacctatt
      781 cgcttccgaa ggagcggttc gcagactggt tcaaccagaa agtgcggcat accactacct
      841 cgaaatatta caagggtggt catcgtttcc agttattcct ggcagggatt tgtccgatgc
      901 tggtctggct gagtctgatc gccctgctgg tatcaggaca gcgcacgaaa gaagccctgg
      961 tggcggccgg cgtcgtgtgg gccctgaaac tgcctatctt tgcatatagc gcgtcgaagc
     1021 tgcgggagaa agacctcatc tggctctatc ccttgctgga actgctgtat agcttcctcc
     1081 tcccgatcat ctacctcgct caactcctta ccaaaaaacg cgcatggaag taaatccgaa
     1141 tctctccgaa aaagcccagg ttgatttcaa gctggtgcaa caagctgtca agggcgacca
     1201 gaaggcttac gccgaactga tgtcgcgcta caaggacagc atttacttca tgctgttgaa
     1261 aatggtcaac aaccgcgacg acgcggagga tctgaccatc gaagcgttcg gtaaggcgtt
     1321 caagaacctg caccagtaca cacccgactt cgccttcagc acctggctgt tcaagatcgc
     1381 gacgaacaat tgcatcgact tcatccgccg caagcggaag tacacgttct cgatcgataa
     1441 gagcatggag aacgacagtg gtcaggagat gcagttcgag atcaagtcgc cgatgctcga
     1501 cccggaggag aagatgatca agaagcagaa ggccgcgctc atgcgcgacg tggtcgagaa
     1561 gctcaagccc cgctacaagc gcctggtgga gttgcgttat ttccaggaac gttcctacga
     1621 agagatcgcc gacgaactcc agcttcccct cggcaccgtc aaagcccaac tctttcgcgc
     1681 gcgggagttc ttgtatcaga tcctgaagaa tcaggaagaa cagatttgag gtttggggtt
     1741 tcgggttggg agtttgaagc tggtaattgg taattagtaa ttagtaacta gtaactggta
     1801 actggtaact ggttgctcgt ccctcgtccc tcgtccctcg tcccacgtcc ctcgtccctc
     1861 gtcccccgtc ccttgcttct cctcgaacgc tatttccccg aactgacgca acagcaacgt
     1921 gaccgcttcg cggccttgcg ggagttgtac ggtgagtgga acgcgaagat caatgtggtg
     1981 tcgcggaagg acatggagaa cctgtacgag cggcacgtgt tgcattcgtt ggggatcgcg
     2041 aaggtgatca gtttcgcggc gggtacgcgg gtgattgacg tgggaaccgg cggcgggttt
     2101 ccggggattc cgttggcgat acttttcccc gacagtcagt tccacctgat cgattcgatc
     2161 ggaaaaaaga tcaaagtggt ggagggcgtt gcggaggcct tggggttaac gaacgtcagt
     2221 gcccagc
//
LOCUS       DAOTON010000488         2220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_38218_length_2220_cov_3.732564, whole genome shotgun sequence.
ACCESSION   DAOTON010000488 DAOTON010000000
VERSION     DAOTON010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38218_length_2220_cov_3.732564"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="P5292_09490"
     CDS             <1..203
                     /locus_tag="P5292_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39399.1"
                     /translation="TTGIIRVTGLFPDWMQVTDLSGREVYAIAHTDQLDLGALPEGIY
                     LLRLQFGDRSHCSLVVKNGHRR"
     gene            370..1482
                     /locus_tag="P5292_09495"
     CDS             370..1482
                     /locus_tag="P5292_09495"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRS39400.1"
                     /translation="MDHQLKFVNSSHTRFFLELRKRVDAYFTSNGISPHANAAMVFKT
                     IFMLALYFVPYALLISGTATGWGFWACWALMGFGLAGIGMSVMHDANHKAYSSSDTVN
                     YIMGKTLNVVGGDARNWKAQHNILHHTYTNVYNADEDVNDKLGMRFSPAGKHRSIQRY
                     QFLYAFVLYSIMTFYWVLLKDLIQHFRYQRKGLNKETGWKKVGTILSLVVWKLFYVGY
                     MLVLPALVLEVSFLKLFAGFLLLHLIAGTVLSVVFQLAHVVEAAEFPMPDAEGNLETE
                     WAIHQLRTTADFAPGNPIITFYVGGLNYQAIHHLFPRICHIHYPKLAPIVAQTAKEFG
                     VPYTVYPSFGKALRSHVRMLQKLGRKDYFHLIETMG"
     gene            complement(1554..2129)
                     /locus_tag="P5292_09500"
     CDS             complement(1554..2129)
                     /locus_tag="P5292_09500"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39401.1"
                     /translation="MNIDTCNAYQSSLTFPIDVGQNLPSGITGYDHPSIPALHGLVQA
                     VTGINQGLIAYTMNATMDSVLNKQYYLRYEYGRVRDACAGPDGSLYFIAYDRSSPRIM
                     RVRNALNTGVSSTTFPEIAVFPNPCTDRIRFSTALPPTTTSAVLRNIQGEIVLHQKVE
                     GNSDLLDVGMLPAGVYYLQVGNSVPKMLLKL"
BASE COUNT          502 a          533 c          607 g          578 t
ORIGIN      
        1 cgaccaccgg aataattcgg gtcacaggac tctttcccga ttggatgcag gtaaccgatc
       61 tcagcggtcg ggaagtttac gcgatcgcgc ataccgatca actcgacctt ggagccttgc
      121 cggagggaat ctacctgctg cgcttgcagt tcggtgatcg tagccattgc agtttggtcg
      181 taaaaaatgg acatcgtcgt tagagttgat gcccatgcag ttccgggtat cgcagggatt
      241 tagaggaaga atgctccgaa tctttgaaat tgcagagaaa tgtcagggtt aaactgccgt
      301 ttttttactt acttgccgaa ataggtcgag ggaaaacttt gtatttttcc cacaaattcc
      361 agtattatta tggatcatca actgaaattc gtcaattcca gtcatacccg attttttctc
      421 gaattaagaa agcgagttga tgcctacttc acctccaatg gtatcagtcc gcatgcgaat
      481 gcagcgatgg tattcaaaac catcttcatg ctggctttgt atttcgttcc gtatgccttg
      541 ttgatcagcg gaacggctac cggttgggga ttttgggcct gctgggcgct gatgggcttc
      601 ggccttgccg gaatcggcat gagcgtcatg cacgatgcca atcacaaggc ctattcgtcg
      661 agcgacaccg tgaactatat catgggtaag accttgaatg tagtaggagg agacgcgcgc
      721 aactggaagg cccagcataa tatcctgcat catacctata cgaatgttta caacgcggac
      781 gaagacgtga acgataagtt ggggatgcgg ttctcgccgg ccggtaagca ccgttcgatt
      841 cagcggtacc agtttctgta tgccttcgtg ctctattcga tcatgacctt ttactgggtg
      901 ctgctcaagg atctgattca gcatttccgt tatcagcgca aaggattgaa taaagaaacc
      961 ggttggaaga aagtgggcac catcctttcg ctggttgtgt ggaagctttt ctatgtaggc
     1021 tatatgctcg tattgccggc actcgtactg gaggtttcat tcctgaaatt attcgccggc
     1081 ttcctgttgc ttcacctgat cgccggcacg gtactgagtg tggttttcca gcttgcgcat
     1141 gtcgtggaag cagcggaatt tcccatgccg gatgcggaag gcaacctcga gacggaatgg
     1201 gccattcacc agttgcgcac cacggccgat tttgccccgg gtaatccgat cattactttt
     1261 tatgtcggcg gactgaatta ccaggccatt catcacctct ttccgaggat ctgtcatatc
     1321 cattacccca agctggcgcc catcgttgca cagacagcga aggagttcgg tgttccgtat
     1381 acggtttatc catctttcgg aaaggcgctt cgttcacatg tgcggatgct gcagaagctg
     1441 gggcggaagg attatttcca cctgattgaa acgatgggtt gatcggttga tcggtgtgcc
     1501 ttgtattgag gcacacggag cacacggatc gtacggattt tcacggaatt tacttacaac
     1561 tttagcagca ttttcggaac gctgttgccg acctgcaggt agtacactcc tgccggcaac
     1621 atcccgacat ccagcaggtc ggaatttcct tcgacttttt ggtgtaacac gatttcgcct
     1681 tgtatgttgc gaaggacggc ggaggtggtc gtgggaggaa gtgcggtcga aaaccggatt
     1741 cgatcggtac atggattcgg aaataccgct atctcgggga atgttgtgga tgacacgccc
     1801 gtattcaacg cgttccgcac ccgcatgatc cgcggtgagg aacggtcgta ggcgatgaaa
     1861 tacaaacttc cgtcggggcc agcgcaggca tcgcggacgc gaccgtactc gtaacgcaga
     1921 tagtactgct tgttgaggac ggaatccatc gttgcgttca tcgtgtaggc gatgagtccc
     1981 tgattgatgc cggtcaccgc ctgtaccagt ccgtgcaacg ccggaatcga cggatggtcg
     2041 tacccggtga ttcccgaggg aaggttctgc ccgacatcga tcgggaaggt caggctggat
     2101 tgataagcgt tgcaggtgtc gatgttcacc tggttgaatc cgattcttat tgtcggagca
     2161 atactgctga ataagaacga tcagcggttt ccatagtgaa atgatacaca ccggcggaca
//
LOCUS       DAOTON010000489         2217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_38294_length_2217_cov_2.480574, whole genome shotgun sequence.
ACCESSION   DAOTON010000489 DAOTON010000000
VERSION     DAOTON010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38294_length_2217_cov_2.480574"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="P5292_09505"
     CDS             complement(<1..1352)
                     /locus_tag="P5292_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRS39402.1"
                     /translation="MGIERIRFLATCLFLMSMQTVTAQTGQAKYVVRFTDKNGSTFSV
                     GAPQDFLSQRAIDRRSRQNIPVTQADLPVNEWYIDSVATKGARIINRLKWQNAVVVEC
                     DSLTRISIDALPFVLSSDRVKRVAGSPGDGSRKKSLRLHSGPVLKELPFLRTASYDYG
                     PSFNQLHLMNAEYLHDQGYKGEGIVIAVLDAGFYQADHVPSLDSLFIDNRLLSTWDFV
                     DDQASVFEDDSHGLSVLSTIAGILPGQLIGTAPHAAFHLLRSEDAPTENIIEEYNWAA
                     AAEYADSAGADIITSSLGYSTFDDTSASHTYADMNGNTCPSSLAADFAASRGLLVLAS
                     AGNQGNSGWHYISAPSDGDSVLAVGAVDSSGFKASFSSFGPSSDGDVKPNVAAKGLGT
                     IVSYGDGSNGPGNGTSFACPVLAGAAACLWQAHPNKSNMEVFHAIEQSASYYNTPGDS
                     L"
     gene            complement(1342..2073)
                     /locus_tag="P5292_09510"
     CDS             complement(1342..2073)
                     /locus_tag="P5292_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39403.1"
                     /translation="MIHFAPFKHCRWILLPGLFLIGACTGCKDDTVAPSSLPSYQAYF
                     PIDSGTWIEYDADSVVHLPVDDAGEVDTAVEFYHFQLREVIDSVFIDGEGDTAIRINR
                     YRRENDTLPWEYFNLWTAKRTSENVERTEDNVRFVKLAFPIDARKSWNGNAYNFFLEE
                     VYIYRDLFQAKSVGPYAFDQTITVLQNEFSSNINRIYKEEIYARDVGMIQKQSDSVSV
                     LIQSSGLPLILNGTEYTLTVRDYGH"
BASE COUNT          552 a          576 c          597 g          492 t
ORIGIN      
        1 agggagtcgc cgggagtatt gtaatagctg gcactttgct caatggcgtg aaaaacctcc
       61 atgttcgatt tgttgggatg ggcttgccac agacaagccg ccgctccggc aagtaccgga
      121 catgcgaagg aagtgccgtt gcccggtccg ttactgccat cgccatagga aacgatagtt
      181 ccaagaccct tcgccgcgac attcggttta acatcgccat cggaagacgg gccgaaacta
      241 ctgaaggaag ctttgaatcc ggaggaatcc accgcgccta ctgccagcac actgtcacca
      301 tctgacggag cactgatgta gtgccagccg gaatttccct gattgccggc gctcgccaga
      361 accaggagtc cacggctcgc ggcaaagtcg gctgcaagcg aagacggaca ggtatttcca
      421 ttcatgtccg cgtaggtatg gctggcggaa gtatcgtcga aggtagaata ccccagggag
      481 gatgtgatga tatccgcgcc cgcgctatcg gcatattcgg cagcggcagc ccaattgtat
      541 tcttcgatga tattctccgt cggtgcatct tcgctacgca acagatggaa ggcggcgtgc
      601 ggggcggttc cgattaactg gcccggtaaa atgccggcaa tcgtagataa tacggataat
      661 ccgtgggagt cgtcttcaaa aacggatgcc tgatcgtcga cgaagtccca ggtagataac
      721 aagcgattat cgatgaagag gctgtcgagg gatggtacat gatcggcctg atagaagcct
      781 gcgtccagca cggcgatgac gataccttcc cctttgtatc cctgatcgtg gagatactcg
      841 gcattcatca gatgcaattg attgaaggag gggccataat catacgatgc ggtacgcagg
      901 aaaggcaact ccttcaacac aggtccggaa tgaagccgaa ggctcttttt ccgggaacca
      961 tcgcccggag agccggcaac ccgcttcaca cggtcgctac tgagaacaaa gggcaaagca
     1021 tcaatggaga tccgggtaag ggagtcgcac tccaccacta cggcattctg ccacttgagc
     1081 cggttgatga tccgggcacc tttcgttgca acgctatcga tgtaccattc gttgacaggc
     1141 aagtccgcct gggtcaccgg gatattctgg cgcgatcgcc ggtcgatggc gcgctgggaa
     1201 agaaaatcct gcggcgcgcc gacagaaaaa gtacttccat tcttatccgt aaaacgcact
     1261 acgtattttg cctggccggt ttgcgcagtc accgtctgca tgctcatcag gaacaggcag
     1321 gttgccagga atcggattcg ttcaatgccc ataatctcgg acggttaaag tgtattcggt
     1381 cccattcagg atcagcggca gaccggaact ctgtatgaga acgctgacac tatccgattg
     1441 tttctggatc atcccgacat cccgtgcata gatctcttcc ttgtagatcc ggttgatgtt
     1501 cgaggaaaat tcgttttgta aaaccgtgat cgtttgatca aaggcgtacg gcccgaccga
     1561 tttcgcctga aacaaatcac ggtagatata gacttcttcc agaaagaagt tgtaggcgtt
     1621 tccgttccaa ctcttccggg catcgatggg gaaagccagc ttgacaaacc gaacgttgtc
     1681 ttccgtccgt tccacgttct cactcgtccg cttggcggtc cacaggttga aatattccca
     1741 gggaagcgta tcgttttcac gccgataccg gttgatgcga atggccgtat ccccttctcc
     1801 atcaatgaaa acggaatcga taacttcccg caactggaaa tgataaaatt caacggccgt
     1861 gtcgacctcg cctgcatcat caaccggaag atgaacaacc gaatcagcgt cgtactcgat
     1921 ccaggtaccg ctatcaatgg ggaagtaagc ctggtaagat ggcaaactgg aaggtgcaac
     1981 cgtatcgtcc ttacaacctg tgcatgcacc aatcaggaaa agtcccggca acaggatcca
     2041 tcggcaatgt ttgaatggag caaaatggat catggttcag gtagcgtgaa tttgctgaag
     2101 atactgctcc agtcgtgggt tgacaatctt gaaccaaatg ggaggaataa gcgccatcag
     2161 gactgcagat gcatacccac cgggaagtac aggacttttt tcgtacgtct gaagggt
//
LOCUS       DAOTON010000490         2213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_38385_length_2213_cov_3.629286, whole genome shotgun sequence.
ACCESSION   DAOTON010000490 DAOTON010000000
VERSION     DAOTON010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38385_length_2213_cov_3.629286"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="P5292_09515"
     CDS             <1..422
                     /locus_tag="P5292_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HRS39404.1"
                     /translation="NPACAPEGCENIFVLCPVPDLRYKPDWSDSDKLADTILRDLSQR
                     TGFDFVANTVTRTIWTPLDWEEKFNLYRGSGLGLAHGLNQVGGFRPSNKDESFENLYY
                     VGASTTPGTGLPMVVISSRLAVERIEHEHAAVHGNVL"
     gene            394..1227
                     /locus_tag="P5292_09520"
     CDS             394..1227
                     /locus_tag="P5292_09520"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRS39405.1"
                     /translation="MLQYTETCYKLSKTITRDYSTSFSLGIHMLDRSYHPAIYAIYGF
                     VRLADEIVDTFHGHDKSALLEKFKAETDAALRDRVSTNTVLHAFQDVVHRYAIERELI
                     DAFLHSMEMDLQPVRYDQNLYHEYIYGSAEVVGLMCLRVFCAGDEQAYQSLREPARRL
                     GAAFQKVNFLRDIRSDFDERGRVYFPGVDFSRFDNAAKQQIEPDIEADFTAALPGIQA
                     LPKGARLGVMVAYIYYRALFRKIRLLPAQRIRQERIRVPNTQKFLLLIQTWLRHRFKL
                     V"
     gene            1230..1718
                     /locus_tag="P5292_09525"
     CDS             1230..1718
                     /locus_tag="P5292_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39406.1"
                     /translation="MRGSILLFLLLPLLSAAIQPEEVRKLFRQASQDEAVAEQLIKTL
                     SGQQDQPVLQAYFAATTALLGKYALNPYTKLSHCKQSSEVFQKAIAADPGNVEIRYLR
                     LAIQLNLPKFLGMSNDLEEDRRVILQGLPFLRDISLRNEIAQFLLAQHQCTRAELACC
                     LP"
     gene            1706..>2213
                     /locus_tag="P5292_09530"
     CDS             1706..>2213
                     /locus_tag="P5292_09530"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HRS39407.1"
                     /translation="MSAVSMDPRYTYLLINLGSILVPFIASFEPRLRFYRQWKALLPA
                     LLITLVFFIVWDHYLAAWDVWGFNPKYVLGIYWWGLPMEEWLFFITIPYSCLFIYASL
                     NYLLPREPFSRHARNITGAWVVILLFVAALGYDRLYTGIKLTLTAVLLAYAWYRDYAW
                     MGKFIRCYL"
BASE COUNT          459 a          680 c          582 g          492 t
ORIGIN      
        1 cgaatccggc ctgcgcgccg gaaggctgcg aaaacatctt cgtgctgtgt ccggtaccgg
       61 acctgcgcta caagcccgac tggagcgact cggacaagct tgccgacacc atactgcgtg
      121 atctgtcgca acgtaccggc ttcgacttcg tcgcgaacac cgtgacgcgt accatctgga
      181 cgccgctgga ttgggaagaa aaattcaacc tctaccgcgg cagcgggctt ggtctggcgc
      241 acggattgaa ccaggtcggc ggattccggc catcaaacaa agacgagtca tttgaaaacc
      301 tatattacgt gggcgcgtcc acgactcccg gcaccggtct gccgatggtg gtgatcagtt
      361 cgcgcctggc cgttgaacgc atcgaacatg agcatgctgc agtacacgga aacgtgctat
      421 aagctcagca agacgatcac gcgcgattac agcacctcgt tttcgctcgg gatccacatg
      481 ctggatcgtt cctaccaccc cgcgatctac gcgatctatg gttttgtgcg gctggcggac
      541 gagatcgtgg acacctttca cggtcacgac aaatccgcct tactcgaaaa gttcaaagcg
      601 gagaccgatg ccgccctgcg cgaccgggtg agtacgaata ccgtcctgca cgccttccag
      661 gatgtcgtac accgctacgc gatcgaacgt gaattgatcg acgcgttcct gcacagcatg
      721 gagatggacc tccaaccggt tcgttatgat cagaacctct accacgaata catctacggt
      781 tccgccgaag tggtgggcct gatgtgcctg cgcgtctttt gcgcgggcga cgagcaagcg
      841 taccagtcgc tgcgggagcc ggcccgccgc ctgggggcag cgtttcaaaa ggtgaatttc
      901 ctccgcgaca tccgaagcga cttcgacgag cggggtcgcg tctatttccc cggcgtcgac
      961 ttttcccgtt tcgacaatgc cgcgaagcaa cagatcgaac ccgacatcga agcggatttt
     1021 acggccgcgc ttcccggcat tcaagcattg ccgaaaggcg cccggcttgg ggtcatggtt
     1081 gcgtacattt actaccgggc cctgttccgg aaaatccgcc tgcttcccgc gcaacgcata
     1141 cggcaggagc gcattcgcgt acccaatacg caaaaattcc tgttgctgat ccaaacctgg
     1201 ttgcgccatc gtttcaagct ggtctgatca tgcgcgggtc catcctactc ttcttattgc
     1261 tacccctcct atccgccgcc attcaaccgg aagaagtgcg caaactattc cggcaagcga
     1321 gtcaggacga ggccgtagcg gaacaactga taaaaacact cagcggacag caagaccaac
     1381 ccgtgttgca agcctacttt gccgctacga cggcgctgct gggcaagtat gcactgaacc
     1441 cctacaccaa gctcagtcat tgcaagcaat cttcggaagt cttccaaaaa gcgattgctg
     1501 cggatcctgg taatgtagag atccggtacc tacggcttgc cattcaactc aaccttccga
     1561 aattcctcgg catgagcaac gacctggaag aagaccgtcg tgtgatcctc cagggcttgc
     1621 cgttccttcg cgacatttca ctgcgcaatg aaatcgcgca gttcctactc gctcaacatc
     1681 aatgcacgcg ggcggaatta gcctgttgtc tgccgtaagc atggacccgc gctacaccta
     1741 tctactcatc aacctgggtt ccattctggt gcccttcatc gcctccttcg aaccccggct
     1801 gcgcttttac cggcaatgga aggcgctgtt gcccgcgctg ctgatcaccc tggtcttctt
     1861 catcgtctgg gaccattacc tggctgcctg ggacgtctgg ggtttcaatc cgaagtatgt
     1921 actgggcatt tactggtggg ggttgccgat ggaagaatgg ctttttttca tcaccatacc
     1981 ttattcctgc ctgttcattt acgcttcgct caactacctc ctgccgaggg aaccctttag
     2041 ccggcacgcc cgaaacatta ccggcgcatg ggttgttatc ctcctgttcg ttgccgccct
     2101 tggctacgac cggttgtata ccgggatcaa actgacgctg accgcggtcc tgctggcgta
     2161 tgcctggtac cgcgattatg cctggatggg gaaattcatc cgctgctacc tcg
//
LOCUS       DAOTON010000491         2213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_38389_length_2213_cov_2.925857, whole genome shotgun sequence.
ACCESSION   DAOTON010000491 DAOTON010000000
VERSION     DAOTON010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38389_length_2213_cov_2.925857"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="P5292_09535"
     CDS             <1..1097
                     /locus_tag="P5292_09535"
                     /inference="COORDINATES: protein motif:HMM:NF023503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HRS39408.1"
                     /translation="TADPEKPEEGKRTWEMVNFYHNPVVATVALLTKLQSDVRNAESQ
                     VIDELLTSVDKNIVKLDKLNAKVIANSTVVTIGSEFQAVVFLSATSSTMAPEVFIGAT
                     YDSTSKACKGCDGNALPVEGGYAKYSDRPGSEGEKKWSGVIRVKKPDGSYDHYPFSSS
                     YVAQKPNSVVSADNMNVLYIGVENPMSISVPGVTHDKVKVTIEGAGGALRPNPSAGAG
                     RYTATVGSVGKATIRVSAEIGGKVMPMGAFEYRVKRVPDPVATISNSKGGNIGKNLLM
                     AGTLIPILENFDFKLFFKITGFKMTITGKGKDLVEYETQGNQLSQPMRDALSKTRAGD
                     KVFFEYIKARMDGGNDPSPRNLSPMAFTIQ"
     gene            1122..1979
                     /gene="gldN"
                     /locus_tag="P5292_09540"
     CDS             1122..1979
                     /gene="gldN"
                     /locus_tag="P5292_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HRS39409.1"
                     /translation="MKFRILLVLLLVALAGNVSAQTGGPNVLDGVYVKEHTIERTPLP
                     YAYLREADVMWSKRIWRIIDLNEKMNLPLKYPLSKQTQDRKSLIDLVMDAVQEGSLTA
                     YTFADDEFTAPVTMKEVESRGGARTDTVKMQRPEPPYDEYDTIIAREFSRDKVIGFRM
                     KEDWFFDKQRSVMEVRIIGIAPLIYAVDQDGNVREGNIKIPLFWIYYPEARKLFANAE
                     VFNRSNDAERRSFDDIFQKRLFASYIYKESNVYDRRIDDYKQGMNALLESERIKTEIT
                     NMEHDMWEF"
     gene            complement(2051..>2213)
                     /locus_tag="P5292_09545"
     CDS             complement(2051..>2213)
                     /locus_tag="P5292_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRS39410.1"
                     /translation="DVCKSVNDGTYFVSRPDEVQAGWFSPNDRVGICGATSTPMWLME
                     AVRDRLHSF"
BASE COUNT          518 a          639 c          632 g          424 t
ORIGIN      
        1 acacggctga tccggagaaa cccgaggaag gcaagcgcac ctgggaaatg gtgaacttct
       61 atcacaaccc ggtcgtagcg acggtcgccc tgctcacgaa actgcagagt gacgtccgca
      121 acgcggaatc gcaggtgatc gatgaactgt tgacctccgt cgacaagaac atcgtgaaac
      181 tggataagct caacgccaag gtgatcgcca acagtacggt ggtgaccatc ggctcggagt
      241 tccaggcggt tgtgttcctg tcggctactt cgtcaacgat ggcgcccgaa gtcttcatcg
      301 gcgctaccta tgactcgacc agcaaggcct gcaaaggctg cgacggtaac gcgctgccgg
      361 tagaaggcgg ctatgccaaa tattccgatc gtccgggttc cgaaggcgag aagaagtgga
      421 gcggtgtgat ccgtgtcaag aagcccgacg gctcctacga ccactatccg ttcagctctt
      481 cctacgtagc gcagaaaccg aactcggttg tgtccgccga caacatgaac gtgctttata
      541 tcggcgttga aaacccgatg tcgatctcgg ttcccggcgt tacgcacgat aaagtgaaag
      601 tgaccatcga aggtgccggt ggcgcgctgc gtccgaatcc ttccgccggt gcgggtcgtt
      661 ataccgctac ggtcggatcg gtcggcaagg ctaccatccg cgtttccgcc gagatcggcg
      721 ggaaggtcat gccgatgggc gcgttcgagt accgcgtgaa gcgtgtaccg gatccggtcg
      781 ctacgatctc gaacagcaag ggcggcaaca tcggcaagaa cctgctcatg gcaggtacgc
      841 tgattccgat cctggaaaac ttcgacttca aattgttctt taagatcacg ggcttcaaga
      901 tgaccatcac cggtaagggc aaggacctcg tcgaatacga gacgcagggt aaccagctca
      961 gccagccgat gcgcgacgcg ctttccaaga cgcgcgcggg cgataaggtc ttcttcgaat
     1021 acatcaaggc gcgcatggac ggcggtaacg acccgtctcc gcgtaacttg tccccgatgg
     1081 ccttcaccat ccagtaatag cagtacctaa aataattcgt catgaaattc cgcatcctgt
     1141 tggttctcct gctcgtcgca ctggccggca acgtttccgc ccaaaccggc ggcccgaacg
     1201 tgctcgacgg cgtctacgtg aaggagcata ccatcgaacg cactccgctg ccttacgctt
     1261 acctgcgcga ggcggacgta atgtggagca aacggatctg gcgcatcatc gatctcaacg
     1321 agaagatgaa cctgccgctc aagtacccgc tgagcaagca gacccaggac cgcaaatcgc
     1381 tgatcgacct ggtaatggac gccgttcagg agggttcgct caccgcgtac accttcgccg
     1441 acgacgaatt cacggcaccg gtcacgatga aggaagtgga gtcacgcggc ggcgcacgga
     1501 ccgatacggt caagatgcag cgtccggaac ctccctatga cgaatacgat acgatcatcg
     1561 cgcgggaatt ctcccgtgac aaggtgatcg ggttccgtat gaaggaagac tggttcttcg
     1621 acaagcagcg ttcggtcatg gaagtccgca tcatcggcat cgcgccgctg atctacgcgg
     1681 tcgaccagga cggaaacgtg cgcgaaggca acatcaagat cccgctgttc tggatctact
     1741 atccggaagc gcgcaagctg ttcgccaacg ccgaagtctt caaccgttcc aatgacgccg
     1801 agcgacgctc gttcgacgac atcttccaaa agcgactctt tgcttcgtac atctacaagg
     1861 agtccaacgt gtacgaccgt cgtatcgacg actacaaaca gggcatgaac gccttgctgg
     1921 agtcggagcg gatcaagacg gagatcacga atatggaaca cgatatgtgg gaattctaac
     1981 accggaaagt ccggttgaca atggtagaca ccggtcgcag ttgcggccgg tgttttttta
     2041 ttcccgaaaa tcaaaaggag tgcaaacggt cgcgaacggc ttccatcagc cacatcggcg
     2101 tggaggtagc gccacagatg ccgacccggt cgttcggcga aaaccaccct gcctgcactt
     2161 cgtccgggcg gctgacgaag taggtgccgt cgtttacgga tttgcagacg tcg
//
LOCUS       DAOTON010000492         2207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_38530_length_2207_cov_3.035316, whole genome shotgun sequence.
ACCESSION   DAOTON010000492 DAOTON010000000
VERSION     DAOTON010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38530_length_2207_cov_3.035316"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /gene="asnS"
                     /locus_tag="P5292_09550"
     CDS             <1..797
                     /gene="asnS"
                     /locus_tag="P5292_09550"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRS39411.1"
                     /translation="FGKSTNLTVSGQLEGELAATALGNIYTFGPTFRAENSNTPRHLA
                     EFWMIEPEMAFYDIRDNQDLAEDFLKYLVRYALEHGRDDIEFLAKTYDDQLIPRLQMV
                     VDHPFERLTYTTAIEILKNSGKKFEFPVNWGTDLQKEHENFLVDHFKRPVILTDYPKD
                     IKAFYMKVNEDGKTVRAMDILFPWIGEIIGGSQREERLEVLQNRMREMHVPESELWWY
                     LDTRKFGTCVHAGFGLGFERLMLFVTGMTNIRDVIPFPRTPKNAEF"
     gene            complement(895..1419)
                     /locus_tag="P5292_09555"
     CDS             complement(895..1419)
                     /locus_tag="P5292_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39412.1"
                     /translation="MNKHSTLIIVAIFLACLIGALAASAQYTASLGVRAGKFATGFTG
                     KYFFDTNGNTGVEAWAAYTKEANDGYTARAFFIKQQSIFNSRLQIPVDFIAGAGIHAG
                     YFKDRYYDVVEGDAKYYNQSTFAGGVGATIQLEYNSRRFPMTLGIDAHPFYNIISPGP
                     EWIDFGVNLRYKIF"
     gene            complement(1825..>2207)
                     /locus_tag="P5292_09560"
     CDS             complement(1825..>2207)
                     /locus_tag="P5292_09560"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRS39413.1"
                     /translation="PHVPGYTASYGILLDMVGARGAKFRQEGYSREAAGYIVDKVWST
                     ANANGFSDYFPYQPGGWVTDDHVYVNRMGIPSIDIIGSDPTAVSGFGKHWHTHRDSIE
                     IIDPATLHAVGQTLLAVLYGNPNP"
BASE COUNT          480 a          585 c          699 g          443 t
ORIGIN      
        1 ttttcggtaa gtcgaccaac ctgaccgtat cgggccagtt ggaaggcgag ctggcggcga
       61 ccgcgctggg taacatttac accttcggtc cgactttccg cgcggagaac tccaacacgc
      121 cgcgtcacct ggcggagttc tggatgatcg agccggagat ggcgttttac gatatccgcg
      181 acaaccagga cctggcggag gattttctca agtacctggt gcggtacgcg ctggagcacg
      241 gtcgcgacga catcgagttc ctggccaaga cctacgacga tcagttgatt ccacgtttgc
      301 aaatggtcgt ggatcacccg ttcgagcggc tcacctatac gacggcgatc gagatcctga
      361 aaaactcggg caagaagttc gagttcccgg tgaactgggg cacggacctg cagaaggagc
      421 acgagaactt cctggtggac cacttcaaac ggccggtgat cctgacggat tatccgaagg
      481 acatcaaagc attctacatg aaagtgaacg aggacggcaa gaccgtgcgc gcgatggaca
      541 tcctgttccc gtggatcggc gagatcatcg gcggatcgca gcgggaggaa cggctggagg
      601 tattgcagaa ccgcatgcgc gagatgcacg tgccggaatc cgagctgtgg tggtacctcg
      661 acacgcgcaa gttcggcacc tgtgtacacg ccggcttcgg actcggtttc gagcgcctga
      721 tgctgttcgt cacgggtatg accaacatcc gcgatgtcat tccgttcccg cgcacgccga
      781 agaacgcgga gttctgatcc ggttacagca aagcgaaaac cccggtcggt gcaggacacc
      841 agccggggtt ttctttttca ggaactacac gcgcctgaaa aatgaatcct aacctcagaa
      901 gattttatag cgcaggttca ctccgaagtc gatccattcg gggcccggac tgatgatgtt
      961 gtagaacgga tgggcgtcga tgccgagcgt catcgggaaa cggcgcgagt tgtactcgag
     1021 ttgtatcgtc gcgcctacgc cgccggcaaa ggtactctgg ttgtaatact tcgcatcgcc
     1081 ttccactacg tcgtagtaac ggtccttgaa gtaacccgca tggatacctg caccggcgat
     1141 gaagtcgacc ggaatctgca ggcgggagtt gaagatcgat tgttgtttga tgaagaacgc
     1201 gcgggcggtg tagccgtcgt ttgcttcctt ggtgtaagcc gcccaggctt ccacacccgt
     1261 gttaccattc gtgtcgaaga agtacttacc ggtgaaaccg gtggcgaatt tgcccgcgcg
     1321 cacacccagg ctggcggtgt attgcgcgct cgcagccaga gcgccgatca ggcaggcaag
     1381 gaaaatggca acgatgatca gagtcgaatg tttgttcatg caaagggagt taaggatccg
     1441 attgcaagga acgacctgca ggaacgcaag ggggatcgtt cttcggcagg acttactgtt
     1501 gtaagattcc gggtgtttcc gggatcattc cggagtaaac agccggaata gcacctggaa
     1561 atggccggta aaggattaac ggccggtaag aaggaggacg aaatcgaccg aacaaaccgg
     1621 agtgagtctg gatttcgttt aaggaaagtt gccggccgat ggtgacgact gaaccttact
     1681 gttgagcgga ccggaggcgt ggcaggctag ccccgggcga gcgagcctcc tgtgcgcctt
     1741 gaggaaagaa acactgttgc tgtggcgagc gagcacggga cgggtgccgg tgccagcacg
     1801 cccgcaccgc tccaacttcc catctcaggg attcggattg ccgtagagta ccgcgaggag
     1861 ggtttgtccg acagcatgca gggtggcggg gtcgatgatc tcgatcgaat cgcggtgggt
     1921 gtgccagtgc ttgccgaatc ccgagacagc cgtgggatcg gagccgatga tgtcgatcga
     1981 cgggatgccc atgcggttga cgtagacgtg atcgtcggtg acccatccgc cgggttgata
     2041 cgggaagtag tcgctgaaac cgttcgcgtt ggcggtgctc cagaccttgt cgacgatgta
     2101 accggccgct tcgcgtgaat atccttcctg ccggaacttc gcgccgcgcg cgccgaccat
     2161 gtcgagtagg atgccgtacg aagccgtata gccgggcaca tgcggtt
//
LOCUS       DAOTON010000493         2204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_38603_length_2204_cov_2.791996, whole genome shotgun sequence.
ACCESSION   DAOTON010000493 DAOTON010000000
VERSION     DAOTON010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38603_length_2204_cov_2.791996"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>531)
                     /locus_tag="P5292_09565"
     CDS             complement(<1..>531)
                     /locus_tag="P5292_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRS39414.1"
                     /translation="DYKIEFCLADRDPSGAVTNGITRTQTSITEFGPDNLMKSAASGG
                     HDPWNPQKYLNLWVCNLGGGLLGYAQFPADLATSPQTDGVVIGYTYFGTIGTATAPFN
                     KGRTATHEVGHWLNLRHIWGDDVCGDDFVGDTPTQEASNAQCPSFPHVTCSNGPNGDM
                     FMNFMDYVNDPCMYFFS"
     assembly_gap    532..541
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<542..1147)
                     /locus_tag="P5292_09570"
     CDS             complement(<542..1147)
                     /locus_tag="P5292_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39415.1"
                     /translation="MKKNLLFSALLLLSGKALLGQCPNDNTAYLSGAAPTVVGNSVVA
                     QQTWAGEFNRVTGMVAGNTYRISTCSTATFDSEITIYPANGGSPVGYDDDGCGVAGGP
                     SSIDFVPAVTGDYDILLDQFQCQNNQIDMDVTITLIATGGGSSNPPDLTIPVVVHVVY
                     NTAGQNISDAQIASQLEALNADYRKINSDLSLVPSVYRSLAA"
     gene            complement(1158..1538)
                     /locus_tag="P5292_09575"
     CDS             complement(1158..1538)
                     /locus_tag="P5292_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39416.1"
                     /translation="MKTLTCLLLLSACSSLALAQRNCGTMQHLDHLRQQDPTLDARHA
                     DYERQLDAWIQEHPQSARPNFPLIPGFVPTGDADQDRVAYAKAKAVLVADPATSREWL
                     QPAAPAKRDEKNARPAQFKVITHN"
     gene            complement(1598..>2204)
                     /locus_tag="P5292_09580"
     CDS             complement(1598..>2204)
                     /locus_tag="P5292_09580"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRS39417.1"
                     /translation="HGVTMGEYARMLQGEHWLRTKRTLKLTIIPVANYRHSDLYQLPV
                     APSPNLPNMTAVYLYPSLCFFEGTPVSIGRGTDAPFQVYGYPGFPESDFTFKPISIPG
                     KATRPPFEDTLCSGRDLRRSFRLPESVPTRLELNYLIQCFASWKYRRPFFTPFFDKLA
                     GNESLRQLIFSGWNEADIRKTWEADLQQYKSIRSKYLLYPD"
BASE COUNT          546 a          562 c          598 g          488 t
ORIGIN      
        1 tgagaagaag tacatacaag gatcattgac gtagtccatg aaattcatga acatgtcgcc
       61 attgggacca tttgaacacg tcacgtgcgg gaaggaagga cattgcgcat tcgaagcctc
      121 ctgagtcggc gtatcgccga caaaatcatc accacaaaca tcatcacccc agatatgccg
      181 gagattcagc caatggccga cttcgtgcgt cgcggtacgt cctttattga atggagcagt
      241 tgccgtaccg atcgttccaa aataggtgta cccgatcacc acgccatcgg tctgaggtga
      301 ggtagcaaga tcggcaggga actgggcgta acccaacaat ccacctccga gattgcaaac
      361 ccatagattc agatacttct gaggattcca agggtcatgg ccacccgatg ctgcggactt
      421 catcaggtta tcgggcccga actcggtgat ggacgtctgg gtccgtgtta tgccgtttgt
      481 aacagcgccg gatggatcac gatcggcgag gcagaactcg atcttatagt cnnnnnnnnn
      541 nggctgccag gcttcggtac acagaaggaa ccaacgagag atcggaattg atctttcggt
      601 aatcggcatt cagggcttct aactgagagg cgatctgcgc atcgctgatg ttctgaccgg
      661 cggtgttgta aaccacatgg acaacaaccg gaatggtcaa atcgggagga ttggaactac
      721 caccaccggt agcgatcagc gtgatcgtca catccatatc gatctgatta ttctgacatt
      781 gaaactgatc gaggaggatg tcatagtcac cggtcaccgc aggcacaaaa tcgatggaag
      841 atggtccgcc ggcaacacca caaccgtcgt cgtcgtagcc gaccggagac ccgccattgg
      901 cagggtaaat agtgatctcc gaatcgaagg tagcggtgct gcaggtggaa attcggtagg
      961 tgttcccggc taccatgccg gtgacccggt tgaactcacc cgcccaggtc tgctgggcaa
     1021 ctaccgagtt tcccacaacc gtgggcgcag cgccactcag gtaagcggtg ttgtcgttag
     1081 ggcactgacc aagtagcgcc ttgcctgata aaagcaatag tgcgctgaac agtagatttt
     1141 tcttcatggc tcgttcctca gttgtgggtg attactttga attgagccgg gcgggcgttc
     1201 ttttcatctc gctttgccgg agcggcaggt tgcagccatt cgcgggatgt agcaggatcc
     1261 gcaaccaaga cagccttggc tttggcatat gccacgcgat cctgatcggc atctccggta
     1321 ggcacgaatc cggggatcaa cggaaagttc ggccgagcgg attgcgggtg ttcctggatc
     1381 caggcatcca gttgacgttc gtagtcagca tgccgggcat ccagcgtcgg gtcttgctga
     1441 cggagatgat ccagatgttg catagtaccg caattccgct gggctaatgc caggctgctg
     1501 catgcggata aaagaagcaa acaggttagt gttttcatgg tgtggggata ggtaggtcga
     1561 atttaaccaa gtcggaagcc atctccaaaa aaatacctca atcagggtat agcaggtatt
     1621 tactccggat ggatttgtac tgctgcaagt ccgcctccca ggttttccga atgtcagctt
     1681 cattccagcc gctgaagatc aactgacgta ggctctcatt acccgccagt ttgtcaaaga
     1741 atggagtaaa aaagggacgt cgatacttcc aggaagcgaa acactggatc aaataattta
     1801 attccaagcg ggtcggcaca ctctccggca acctgaacga tcgtcgaaga tccctcccgg
     1861 agcaaagcgt atcttcgaag ggaggccgtg tcgcttttcc gggtatggat attggcttga
     1921 atgtaaaatc cgattccgga aatccgggat aaccgtatac ctgaaacggc gcatccgtac
     1981 ccctgccgat acttactgga gtgccttcaa agaaacaaag cgaaggatac agatacaccg
     2041 ccgtcatatt cggaagattc ggtgaggggg ctacgggcaa ttggtacaga tcggaatgac
     2101 gataattcgc caccggaatg atcgtcaact tcaacgtccg cttcgtcctg agccaatgtt
     2161 caccttgcaa catacgggcg tactccccca tcgtaacgcc gtgc
//
LOCUS       DAOTON010000494         2202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_38639_length_2202_cov_3.321844, whole genome shotgun sequence.
ACCESSION   DAOTON010000494 DAOTON010000000
VERSION     DAOTON010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38639_length_2202_cov_3.321844"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="P5292_09585"
     CDS             complement(<1..1118)
                     /locus_tag="P5292_09585"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRS39418.1"
                     /translation="MIRRLLPGAFLLALLLFSCQPSTKNTSGKSVPVVGFLDLLEDAT
                     LAEAKKGFFVALKDSGFSATDGTLEVIYRNAQNDQPTLLQACDYILSKQPDLIATNPT
                     LSTITAVQRTKDVPVFMMVSPRPDIAGLADAQGNWPANLFGTYETLEYIDTSVLLIKE
                     ILPQAKRIATTYNQSEPQSMDALERIESVCKAQGWTLIKRPVNNSNETQLVVAALLNE
                     KPDAFFALPDNTVFSSMEVIVRACDEAKVPVFTSEEGLVKRGAVAAYGADLYQWGYQS
                     GAMAARMLKQGNADGLKPEALKVARFDDDKVRSLRRVWKESFPVTDGDPVSKYLKGRK
                     LIVPQSSVLRYHPDLTYRDGERLLGKFHAMLALVQAPDG"
     gene            complement(1165..1956)
                     /locus_tag="P5292_09590"
     CDS             complement(1165..1956)
                     /locus_tag="P5292_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRS39419.1"
                     /translation="MKRILGFLLLLLPFLATGQDEDFPARANTLVSDFTGTLAPGERD
                     ALERKLVAFDDSTSTQIAVVIISSVGNYDIADYSVQLFNRWKIGRQDKNNGVLVLVAK
                     DDRKVFITTGYGIEGVLPDILCKRIVDQDIVPNFKAGSFYGGLEQGTNSIMSIVSGEF
                     TADAYMKKGKQAKQFPWFFVLLFILIVFISVIANVRRVSRYASRNNLAFWAAWTLLNA
                     AANRSRGSWGNFSGGSGWGGGGFGGGGFGGFGGGGSGGGGAGGSW"
     gene            complement(1953..>2202)
                     /locus_tag="P5292_09595"
     CDS             complement(1953..>2202)
                     /locus_tag="P5292_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRS39420.1"
                     /translation="ARQFAILGDAGINRKVPEDFWHSIKLEMEHRFALGEFVAGLSNG
                     IERAGAALAEHFPRKHDDKNELPDEIVYGGKLKDRHPR"
BASE COUNT          447 a          654 c          624 g          477 t
ORIGIN      
        1 ccatccgggg cctggacgag ggccagcatc gcgtgaaact tgccgagcag cctttcacca
       61 tcgcgatacg taaggtcggg gtgataacgc aggacgcttg actgcggaac gatcagcttc
      121 cgacccttga gatacttcga caccggatcg ccatccgtca ccgggaacga ttccttccat
      181 acccggcgca gcgaccgcac cttgtcatcg tcgaacctcg caaccttcaa cgcttccggc
      241 ttgagtccgt cagcatttcc ctgcttcaac atgcgtgcgg ccatcgcgcc gctttggtag
      301 ccccactggt acaggtccgc tccgtaagcc gcgacggccc cgcgtttcac gagtccttct
      361 tcggaagtga acaccggcac tttcgcttcg tcgcaggcac gtacgatcac ctccatgctg
      421 gaaaagacgg tgttgtcggg taaggcgaag aaagcatccg gcttttcgtt cagcaaggcg
      481 gccacgacca actgggtttc gttgctgttg ttgaccggac gtttgatcaa ggtccagcct
      541 tgcgctttac atacgctctc gatgcgttcc agcgcgtcca tcgactgcgg ttccgactga
      601 ttgtaggttg tcgcaatgcg cttagcctgt ggcaggattt ctttgatcaa caggacggag
      661 gtgtcgatgt attccagggt ctcgtaggta ccaaagagat tggcgggcca attcccttgc
      721 gcatccgcca gtccggcgat gtcgggtcgc ggactaacca tcatgaaaac aggtacatcc
      781 ttcgtacgct gtaccgccgt gatcgtgctg agtgtaggat tggtggcgat caggtccggt
      841 tgtttggaga ggatgtaatc gcaggcctgc agcagcgtcg gttgatcgtt ctgcgcattg
      901 cgatagatca cctccagtgt gccgtcggtc gcggagaagc cactgtcttt cagcgccaca
      961 aagaacccct tcttcgcttc cgccagcgta gcgtcttcga gcaggtctaa aaagccgacg
     1021 accggaacag attttccgga agtgtttttc gtggaaggtt ggcaggaaaa gagaagaagc
     1081 gccaggagaa atgctcccgg taacagtcga cggatcatac cgcaaggtaa cagaatccgt
     1141 ccgcttaagt tcaggaagcg gaccttacca ggagccgccg gcgccaccgc cgcctgaacc
     1201 tccaccgcca aagccaccga atccgccacc gccgaatcca ccgccacccc agccgcttcc
     1261 tccggagaaa tttccccagg agccgcggct gcgattcgcg gcagcattga gcagggtcca
     1321 ggccgcccag aaggcgaggt tgttccggct cgcataacgg gatactctac ggacgttggc
     1381 aatgacgctg atgaagacga ttaatatgaa caacagcacg aagaaccagg ggaactgctt
     1441 cgcctgtttg cctttcttca tgtaagcgtc ggccgtgaat tcaccgctga cgatcgacat
     1501 gatcgaattg gtgccttgtt cgaggccgcc gtaaaaactc ccggccttga aattcggaac
     1561 gatgtcctga tcgacgatcc gtttacacag gatgtcggga agtacacctt caattccgta
     1621 accggtcgtg atgaagacct tgcgatcatc ctttgcaacg agcaccaaga cgccgttgtt
     1681 cttgtcctgc cggccgatct tccagcggtt gaacagttgc acgctgtaat cggcgatgtc
     1741 gtagttgccg accgagctga tgatgaccac ggcgatctgc gtactcgtcg aatcgtcgaa
     1801 cgcgaccagt ttgcgttcca gtgcgtcccg ctcgccggga gcgagggtgc cggtgaagtc
     1861 gctcaccagc gtattcgccc gtgcagggaa atcctcgtct tgtcccgtcg caaggaaggg
     1921 cagaagcagg agcaggaatc ccaggatacg tttcatcgcg gatgtcggtc cttcagtttg
     1981 ccgccataga cgatctcgtc gggcagctcg ttcttgtcat cgtgtttgcg cgggaagtgt
     2041 tccgccaggg ctgcgcccgc gcgttcgatg ccattggaca gcccggccac gaattctccc
     2101 agcgcgaagc ggtgttccat ctccagcttg atcgaatgcc agaaatcctc cggcactttt
     2161 cggttgatgc ccgcgtcgcc caggatggcg aactgtcgtg ct
//
LOCUS       DAOTON010000495         2200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_38693_length_2200_cov_2.122611, whole genome shotgun sequence.
ACCESSION   DAOTON010000495 DAOTON010000000
VERSION     DAOTON010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38693_length_2200_cov_2.122611"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2200
                     /locus_tag="P5292_09600"
     CDS             <1..>2200
                     /locus_tag="P5292_09600"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS39421.1"
                     /translation="TLDPIPGTGQEITPICSTEVTTCSGGTYTGIQEYVYTGTVTLPG
                     PCADWTFSYNLCCRNAAITNINLPANSLMYIFATLDNTSGICNNSPVFSNKPVPFVCL
                     GQQFCFNHGAYDPDGDSLVYQMITPLDQAGLGVTYLAPFNALNPLSSSPAMTFNPANG
                     DICMTPTNLEVTVMAVLVNEYRNGVLIGSVERDIQVTVINCSNIIPSVSGINGTNSFA
                     TSVCAGSQLCFNVFSSDADAAQNTTLSWDFSIPGATFVTTPGTRESGTFCWTPSAADI
                     STVPYCFTVTVQDDNCPYAGSQTFAYCISVNGLAVNAGPDVALGCTSGATQATLNATI
                     TGVNGPYTYSWSSGQTTSSITVGAGTYIVTATGGGCTGRDTVVVAGGSPNPVAAFSFT
                     DDCTGTIATFTDQSSVSVGGFSGYQWNFGDGTTAAIQNPTHTYSAPGTYNVTLIVQTP
                     GGCSDTITQAVTLTPELPTAAFTAPPVCFGSSTSFTDQSVSNPAITTWQWIFGDGTTS
                     GAASPTHTYADTGSYAANLTITNAAGCTSTVTQTVVVHPLPAPNLGPDVAYCDGGSAT
                     FTATGGGTYAWTPGGMSDSVITVNPTTTTSYTVTVTNVQGCTGTSQVDVIVYPLPVAD
                     FTAPPVCLGAPSVFSDQSVSTQTISNWDWTFGDGTGSTLSSPSYTYADTGTYNAVLTV
                     TTADGCTSSVAQPVIVYPVPTPDLGPGQAYCDGGSATFGTNGGVSYDWTPG"
BASE COUNT          443 a          689 c          584 g          484 t
ORIGIN      
        1 accctggacc ccatccccgg tacgggtcag gagattacgc cgatttgctc tacggaggtt
       61 acgacttgca gcggcggcac gtacaccggc attcaggaat acgtctatac cggtacggtt
      121 acgctccccg gtccttgcgc cgactggaca ttcagctaca atctctgttg tcgaaatgcg
      181 gcgatcacca acatcaacct gccggcgaat tcgctcatgt acatcttcgc caccttggat
      241 aatacttcgg gcatttgcaa taactcgccg gtgttctcga acaagccggt ccccttcgtt
      301 tgtcttggac agcaattctg tttcaaccac ggagcttacg atccggatgg tgattcactg
      361 gtttaccaaa tgatcacgcc cctggatcag gccggactcg gcgtcaccta cctcgcgccg
      421 tttaacgcgt tgaatccgct cagctccagc ccggcgatga cttttaatcc ggccaatggc
      481 gacatctgca tgacgcccac caacctcgaa gtcacggtca tggccgtgct cgtgaacgag
      541 taccggaacg gcgtgctgat cggaagtgtc gaacgcgaca tccaggtgac ggtcatcaac
      601 tgcagcaaca tcattccgtc ggtctccggc attaacggaa ccaatagttt cgcgacttcc
      661 gtctgtgccg gttcgcagct ttgcttcaac gtcttctcgt cggatgccga tgctgcgcaa
      721 aacacgaccc tgagctggga cttttccatc cccggcgcga ccttcgtgac cactcccggc
      781 acgcgggaat ccggtacgtt ctgttggact ccgtcggctg ctgacatctc gacggtaccc
      841 tattgcttca ctgtcacggt gcaggatgac aactgtccgt atgcaggaag ccagacgttc
      901 gcctattgca tcagtgtcaa cggacttgcc gtgaatgcag ggccggatgt cgcactcggt
      961 tgcacatccg gtgcgaccca ggcgacactc aacgcgacga ttaccggtgt gaacgggcct
     1021 tacacctatt cctggagttc cggacaaaca acttcttcga tcacagtcgg tgcgggcacc
     1081 tatattgtta ccgcgaccgg aggaggttgt acgggacggg atacggtcgt agtagccggc
     1141 ggaagcccga atccggtagc ggctttcagt ttcaccgacg attgcaccgg cacgatcgcg
     1201 acgttcaccg accaatcgtc cgtgtcggtc ggcggtttca gcggttacca atggaacttc
     1261 ggcgatggca caaccgcagc gatacagaac ccgacgcata cttacagcgc acccggtacc
     1321 tacaacgtca ccctgatcgt gcagaccccc ggcggttgca gtgatacgat cacgcaggcc
     1381 gtaacgctta ctccggaatt accaacggcc gcgttcactg ctccacccgt ttgcttcggt
     1441 tccagtactt cgtttaccga tcaatccgtt tccaatcccg cgatcaccac ctggcagtgg
     1501 atcttcggag acggcacgac ttccggcgca gcctcaccta cacataccta cgccgatacg
     1561 ggatcatacg cggccaacct caccatcacg aacgcagccg gttgtaccag taccgttaca
     1621 cagacggttg tcgtccatcc gcttcctgcg ccgaacctcg gtccggatgt cgcgtactgc
     1681 gacggcggaa gcgccacctt caccgcaacc ggtggcggca cgtatgcctg gacgcccggt
     1741 ggcatgagtg attcggtgat cacggttaat ccaactacga caacctccta caccgttacc
     1801 gtaacgaatg tacagggttg cacaggcacc agccaggtgg atgtgatcgt ttatcctttg
     1861 ccggttgccg acttcacggc tcctcctgtt tgtttaggcg ctccgagcgt tttctccgat
     1921 cagtcggtct cgactcaaac tatttccaat tgggattgga cgttcggtga cggaaccggc
     1981 agtacactga gttcgccttc gtatacctat gcggatacag gaacctataa cgcagtactg
     2041 acggtcacca cggccgatgg ttgtaccagt tcggtcgcgc agcctgtgat cgtctacccg
     2101 gtgccgactc ccgacctggg tccgggccaa gcctattgcg atggcggcag tgcaacgttt
     2161 gggacgaacg gcggtgtcag ttatgactgg accccgggcg
//
LOCUS       DAOTON010000496         2196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_38777_length_2196_cov_3.542737, whole genome shotgun sequence.
ACCESSION   DAOTON010000496 DAOTON010000000
VERSION     DAOTON010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38777_length_2196_cov_3.542737"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="P5292_09605"
     CDS             complement(<1..516)
                     /locus_tag="P5292_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39422.1"
                     /translation="MSKIKVFSYILLGMAVAGCNGLGKMAKNFNTVKYEVTPNPLEMH
                     GDSVAIAVKGSYPAKYFAKKVDATVTPMVKSSTGEHNFKSVTNVGEKSTTAGNKINYK
                     TGGSFTYSDKIAYTADMKAADVMVKASGTKGKTTKELGSMKIADGTIVTPLLVRNDEK
                     TIVGKDQFVRIV"
     gene            complement(600..>2196)
                     /gene="typA"
                     /locus_tag="P5292_09610"
     CDS             complement(600..>2196)
                     /gene="typA"
                     /locus_tag="P5292_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRS39423.1"
                     /translation="INIIDTHGHADCGGEVERVLTMADGVLLLVDAFEGPMPQTRFVT
                     QKALAAGLKPIVVINKVDKENCRPDEVHEQVFDLFFNLNATEEQLDFPTLYGSSKQGW
                     MSTDWQQPTSDITPLLDAIIEHIPAPVIPEGTPQMLISSLDYSSYVGRIAIGRVFRGQ
                     LRENMPISLVKRDGAITKARIKELHSFEGLGRSKNESVSAGDICALVGLESFDIGDTV
                     ADFENPEPLPPIAVDEPTMSMLFTINNSPFFGKDGKFVTSRHLRDRLFKETEKNLALR
                     VDETASPDAYNVYGRGILHLSILIETMRREGYELQVGQPQVIIKEVDGERMEPMEALT
                     VNVPEQFSGAVIDLVTQRKGELQIMEPKGDVLHLEFSIPARGIIGLRNSLLTATQGEA
                     IIAHRFKAYEAWKGEIPGRINGVIISMDKGKSTAYSIEKLQDRGRFFIDPTEDVYMGQ
                     IIGEATRPGDLVVNVTRAKQLTNFRAAGSDDNVRITPKIDFSLEEAMEYIEKDEYLEI
                     TPKAIRMRKILLDENDRKRAAKN"
BASE COUNT          485 a          576 c          551 g          584 t
ORIGIN      
        1 cacgatgcgt acgaactgat ctttaccgac gatcgtcttc tcgtcgttac ggaccagcag
       61 cggggtcacg atcgttccgt cggcgatctt catcgaaccg agttccttcg tggtcttgcc
      121 tttggtaccg gaagctttca ccattacatc agcggctttc atatcagccg tgtaagcgat
      181 cttgtcgcta taagtgaagc tgccacccgt cttgtagttg atcttgtttc cggcggtggt
      241 ggatttttcg ccgacgttgg tcaccgactt gaagttatgc tcgccggtgg acgatttcac
      301 catcggggtg acggtagcgt caaccttttt ggcgaagtat ttagccggat aagagccttt
      361 gacagcgatt gcaacgctgt cgccgtgcat ttccagcggg ttaggcgtga cttcatactt
      421 cacagtattg aagttcttcg ccattttgcc gagtccgtta catccggcta ctgccattcc
      481 cagcaggatg taggagaaaa ctttgatctt actcatggtg tgggtgtgag gactatcgag
      541 gttaggtaat tcgaggccgc aaagattaaa atattttcgt atcgggaaaa tgctgtaggt
      601 taattttttg cagcacgttt acgatcattc tcatcaagga ggattttacg catgcgaatc
      661 gccttcggtg ttatttcgag gtattcgtct ttctcgatgt attccatcgc ttcttcgagc
      721 gagaaatcga ttttaggagt aatgcgcacg ttatcatcac taccggccgc cctgaaattc
      781 gtcagctgtt ttgcgcgtgt aacgttcact accaggtcac ccggacgcgt tgcttcgccg
      841 attatttgac ccatgtagac atcttctgta ggatctatga agaaacgacc ccgatcctga
      901 agcttttcga tggaataagc ggtggattta cccttgtcca ttgagatgat cactccatta
      961 atacgacccg ggatctcccc tttccaggct tcgtatgcct taaagcgatg cgcgatgatc
     1021 gcttcaccct gggttgccgt taaaagagag tttcgcagac cgataattcc acgagcggga
     1081 atactgaact ctaaatgaag aacgtcgccc ttcggttcca tgatctgcag ttcacccttg
     1141 cgttgcgtga cgaggtcgat gacggctcct gaaaattgtt cgggcacatt caccgtcagt
     1201 gcttccatcg gttccatccg ttcgccatcc acttccttga tgatcacctg cggttgtcct
     1261 acctgcaatt catatccctc tctgcgcatc gtttcgatga ggatggacag gtgaaggatg
     1321 ccacggccat atacattgta tgcatccggc gaagcggttt catcaacgcg gagcgccagg
     1381 ttcttctccg tctccttgaa taaacgatcg cggaggtgac gtgaggtcac gaacttgcca
     1441 tcctttccga agaaaggcga attgttgatc gtaaagagca tgctcatcgt cggttcgtca
     1501 accgcgatcg gcggaagcgg ttcgggattt tcaaagtcgg caacggtatc accgatgtcg
     1561 aagctctcca aaccaaccaa tgcacagata tcgccggcgc taaccgactc gttcttgcta
     1621 cgacccaacc cttcaaaaga atggagttcc ttgattctgg ctttggtgat agcgccatcg
     1681 cgtttgacca gggagatcgg catattctcg cgcagttgtc cgcggaatac gcgaccgatg
     1741 gcgatgcggc ccacatagct cgagtaatcg agtgaagaaa tgagcatttg cggcgttccc
     1801 tccggaatca ccggtgccgg aatatgttca atgatagcat cgagcaaagg agtgatgtcg
     1861 ctggtaggtt gctgccagtc ggtgctcatc catccctgct tcgaagaacc gtagagggtc
     1921 gggaagtcca actgctcctc ggtggcattc aggttaaaga acaaatcaaa gacctgttcg
     1981 tgcacctcgt ccgggcggca attctccttg tcaactttat tgatcacgac gatcggtttc
     2041 aaacccgcag ccaacgcttt ttgcgtaaca aagcgggtct gaggcattgg accttcgaag
     2101 gcatccacca acaacaacac cccgtccgcc atggtcagca cccgctccac ctcaccgccg
     2161 caatcggcgt gaccatgtgt gtcgataata ttgatt
//
LOCUS       DAOTON010000497         2194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_38832_length_2194_cov_2.784946, whole genome shotgun sequence.
ACCESSION   DAOTON010000497 DAOTON010000000
VERSION     DAOTON010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38832_length_2194_cov_2.784946"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1077)
                     /gene="dusB"
                     /locus_tag="P5292_09615"
     CDS             complement(94..1077)
                     /gene="dusB"
                     /locus_tag="P5292_09615"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRS39424.1"
                     /translation="MLPKLPDFPLLLAPMEDVSDPPFRFVCKQNGADMMYTEFISSEG
                     LIRHAAKSTMKLDIFEYERPIGIQLFGGEIGSMREAALIAEAAQPDLIDINYGCPVKA
                     VACKGAGAALLQDIPKMVKMTEEIVRAVKLPVTVKTRLGWDDLTKNIVEVAERLQDIG
                     IAALTIHGRTRKQMYKGPADWTLIGEVKNNPRMRIPIFGNGDIDSPIKAVEMRQRYGV
                     DGVMIGRASIGYPWIFREIRHYLNTGEMLAGPTIDERVANCRTHLQKSVEWKGERLGV
                     VEMRKHYANYFKGLDHFRDYRIRLVTLDHLDELHAVLDVIRERYELVNVRP"
     gene            1298..>2194
                     /locus_tag="P5292_09620"
     CDS             1298..>2194
                     /locus_tag="P5292_09620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRS39425.1"
                     /translation="MPLKSIWAQNTPYSKFLLGVGVILISAVAFTLLSTVVVTGLYGI
                     PMYQLENMLQDVKNPLTLQVLKVIQTGSTIGTFIVPAWVLAYLFHGDPKEYLQLRKLA
                     PATSLLWVGLGMLTAVPLINLVGDLNSKMHLPSFLAPVEQWMRDSEDKAAVLTEAFLD
                     MPDPGALLFNLFMIALLPAIGEECLFRGVVQRLFSEWSGKKQVGIWAAALLFSAMHLQ
                     FYGFVPRLLLGALLGYLLLWSGSIWLPMLAHFLNNAAAVIAAYLYQHQLISANPDTLG
                     LGPEQFMVLLASIVLTALCFTLG"
BASE COUNT          431 a          650 c          617 g          496 t
ORIGIN      
        1 taccagaaga gaaagatgct ggtgggggac cgatccatac ggactgcggt tcagcaagtg
       61 ctaaaaacca gcgggtatcc gcatgccgga aaatcaagga cgcacattga ccagttcgta
      121 ccgctcccgg atcacgtcga gcaccgcgtg cagctcgtcc aaatgatcga gcgtcacaag
      181 ccggatgcgg tagtcgcgaa aatgatccag ccctttgaag tagttcgcgt agtgcttgcg
      241 catttccaca acgcccaaac gttctccctt ccactccacg gacttctgca aatgcgtacg
      301 acagttcgcg acgcgttcgt cgatcgtggg tcccgctaac atttcgcccg tgttgaggta
      361 atgccggatc tcccggaaga tccacggata gccgatgctg gcgcgtccga tcatcacgcc
      421 gtccacgccg tagcgttgac gcatctccac ggcctttatc ggcgagtcga tgtcgccgtt
      481 gccgaagatc gggatacgca tgcgcggatt gttcttcact tcgccgatga gcgtccagtc
      541 ggccggacct ttgtacatct gcttgcgcgt gcgaccgtgg atcgtgagcg cggcgatgcc
      601 gatgtcctgg agccgctcgg cgacttcgac gatgttcttg gtcagatcat cccagccgag
      661 acgggtcttc accgtcaccg gcagctttac ggccctgacg atctcttcgg tcatcttcac
      721 catcttcggg atatcctgca gtagcgcggc gccggcgccc ttgcaggcga ccgccttgac
      781 cggacaaccg taattgatgt cgatgagatc cggctgtgca gcctccgcga tcaatgcggc
      841 ttcgcgcatc gaaccgatct cgccaccaaa caactggata ccgatcggac gttcgtactc
      901 aaagatgtca agcttcatcg tactcttggc ggcatgccgg atgagtcctt cgctcgagat
      961 gaactccgtg tacatcatgt ccgccccgtt ctgcttacaa acaaaccgaa acggcggatc
     1021 actgacatcc tccataggag ccaggagcag cgggaagtcg gggagtttgg gaagcatggg
     1081 cggtggttgg taggcggtag gcggtcagca gtaggcagta ggcagttggc ggtaggcggc
     1141 aggcagtatg cagttggcgg ttggccgtga aggggcttgg gggttggggt aaacgttact
     1201 attcgctatt ggctattcgc tattcgccaa cctatttact gttcactgta cactattcac
     1261 ttcataaact accttcgccc cacaaaaata ccgctctatg cccctcaagt caatctgggc
     1321 ccaaaacacg ccgtattcga agtttttact cggagtcggg gtgattttga tctctgccgt
     1381 agcctttacg ctgctcagca ccgtggtcgt gaccggcctg tacggcatcc cgatgtacca
     1441 gttggagaac atgttgcagg acgtgaaaaa cccgctgacg ctgcaagtac tgaaggtgat
     1501 ccagaccggc agcacgatcg gtacgttcat cgttccggcc tgggtgttgg cgtatctgtt
     1561 tcacggcgat ccgaaggaat acctgcagtt gcgcaagctc gctccggcaa cgagcctgtt
     1621 gtgggtgggc ctgggaatgc tcacagccgt gccgctcatc aacctggtgg gcgatttgaa
     1681 cagcaagatg cacctgcctt ccttcctcgc accggtggag cagtggatgc gcgactcgga
     1741 agataaagcg gcggtactca ccgaagcgtt cctcgacatg cccgatccgg gcgcgctgct
     1801 gtttaacctc ttcatgatcg ccttgctgcc ggccattgga gaggagtgct tattccgcgg
     1861 tgtagtacag cgattgttca gcgaatggtc ggggaagaaa caagtcggca tttgggcggc
     1921 ggccttactc ttcagtgcca tgcacctgca gttctatggc ttcgtacccc ggctgttgtt
     1981 gggtgcgctc cttggctact tactactctg gagcggatcg atctggttgc ccatgctcgc
     2041 ccacttcctc aacaacgcgg cggccgtcat cgcggcctat ctctaccaac accaactcat
     2101 cagcgccaac cccgacacac tcggcctcgg cccggaacaa ttcatggtcc tcctcgcctc
     2161 gatcgtcctc accgcccttt gttttacgct gggt
//
LOCUS       DAOTON010000498         2191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_38911_length_2191_cov_2.969569, whole genome shotgun sequence.
ACCESSION   DAOTON010000498 DAOTON010000000
VERSION     DAOTON010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38911_length_2191_cov_2.969569"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="P5292_09625"
     CDS             <1..450
                     /locus_tag="P5292_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS39426.1"
                     /translation="VKKEGYAFESQYVSRKDTARPLVVTSDLQLKKLEVGAQYPINDI
                     LFGTNSSEINDTIKTVLDDFAEYLQLNPKLRVGIQGHTDNVGNPGDNMALSERRAKAV
                     FDYLVGQGITATRMTHKGYGETKPLFPNATEAGRAKNRRTVFVVSQN"
     gene            497..2095
                     /locus_tag="P5292_09630"
     CDS             497..2095
                     /locus_tag="P5292_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRS39427.1"
                     /translation="MKYFLAPVFALLIAIPVSAQQVRSITESDVKSVKVFLNGAQVNR
                     EAKTTIDAGTTLLQIENLSQYIDKSSISVSGTGEAVIAGVGFRMEYLTEDRKSPEVRQ
                     LEDSAKTLQLELDKVNGLEQVYNDEITLLNANKAVGGSNIGVDVDNLREVADFFRQRM
                     IELRIKLIDSGREKKKVSERLARVTQQLNELNARKNHPFGTILVTLTARQRTSVNLSL
                     SYYVQGVAWSPLYDIRAKDVASPVQLSYKAQVTNATGEQWENVRLTLSTGNPSLGGTK
                     PQLNPWYLDFLQPYYGRQKQELKELSAVPAMRDDNLAMGQAVELSAPIVQMNENQLAT
                     DFDITLPYTLNGDGKPVTVDIQSHQLPATYTYYAAPRLDRDAFLLARVTGWDALNLLP
                     GQANVYFEGSYVGETSINPNATQDTLDLSLGRDKRIVITREKLKELTTNKTIGGTQVH
                     ETTWEIKVRNSKREAINLVLEDQFPVSRDKDIKVQTGDFSGASYDEVSGKLTWRLSIG
                     SNQTENRKFNYTVKHPKDRPVPGL"
BASE COUNT          600 a          553 c          574 g          464 t
ORIGIN      
        1 gttaagaaag aaggctatgc gttcgaatca cagtatgtat cacggaaaga cacggcccga
       61 ccattggtag ttaccagtga tcttcaactg aaaaagctgg aagtaggggc acagtacccg
      121 atcaacgata tcctgttcgg aacgaattct tccgagatca acgacaccat taagaccgtc
      181 ctggatgatt ttgcggagta tctgcagttg aacccgaaat tgcgggtcgg catacagggg
      241 catacggata atgtcgggaa ccctggtgat aacatggcgc tttcagagcg tcgcgccaag
      301 gccgtcttcg actatctcgt cggtcagggc attaccgcta cccggatgac gcataaggga
      361 tatggcgaaa cgaagccgct tttcccgaat gctaccgaag ccggacgtgc gaagaaccga
      421 aggacggttt tcgtggtttc ccagaattaa tggtatggaa aatgctatgc accgtgctcc
      481 acttaaaaaa tccagcatga agtactttct agccccggta ttcgctctcc tgatcgcgat
      541 accggtatcc gcacagcaag tgcgatcgat cacggaatcc gacgtgaaat ccgtaaaagt
      601 cttcctcaat ggagcgcagg ttaatcggga agcaaaaacc accatcgatg ccggcaccac
      661 cttactgcag atcgaaaacc tgtcgcagta catcgataag agttcgatca gtgtgagcgg
      721 aacaggagaa gcggtgatcg ccggagtggg tttcaggatg gagtacctga ccgaagaccg
      781 aaaatcaccg gaggtccgtc aactcgagga ctcggcaaaa acgcttcagt tggaattgga
      841 caaagtgaat gggttggagc aggtgtacaa cgatgaaata accctgctga atgccaataa
      901 agcggtgggt ggttcaaaca taggtgtgga tgtcgacaac ctgcgggaag tagccgattt
      961 tttccgacaa cgcatgattg aactgcgcat caaactcatc gattcgggac gggaaaagaa
     1021 gaaagtcagt gaacgcctgg ctcgcgtcac acaacaattg aatgaactga atgcaaggaa
     1081 gaatcatccg ttcggaacca ttctggtcac cctgactgcc cgtcaacgca ccagtgtcaa
     1141 cctttccctg agctactatg tgcagggtgt cgcctggtcg ccgctgtatg atatccgtgc
     1201 caaagacgtg gcgagtccgg tgcagttgtc ttataaagca caggtgacaa atgctacggg
     1261 agagcaatgg gagaatgttc ggctgacact ttcaacaggc aatccgagtc tgggtggtac
     1321 aaaaccacaa ctgaatccgt ggtacctcga ttttcttcag ccctactatg ggcgccagaa
     1381 acaagagttg aaagaactgt cagcggtacc ggccatgcgg gatgataatc tggcgatggg
     1441 ccaggccgtc gagctttccg cgccgattgt tcagatgaat gagaaccaac tggccaccga
     1501 ctttgacatc acccttccgt ataccttgaa cggcgacggc aaaccggtca cggtcgatat
     1561 ccagtcccat caacttccgg ccacctacac ctattatgca gcaccgcgtc tggaccggga
     1621 tgccttcttg ctggcacgtg tcacgggttg ggatgcattg aacctgctgc ctggacaagc
     1681 gaatgtctat tttgaaggaa gctatgtcgg agaaacttcg atcaatccca acgccacaca
     1741 ggacaccctg gatctttcac tcggccgtga taaacggatt gtcattacac gcgagaaact
     1801 gaaagaactg accaccaata aaaccatcgg cggtacgcag gtccatgaaa ctacctggga
     1861 gatcaaagtg cggaactcga aacgcgaggc catcaatctg gtactcgaag atcagttccc
     1921 cgtgagtcgt gacaaagaca tcaaggtaca aaccggagac ttctccggtg cctcgtacga
     1981 tgaagttagc ggcaaactga cgtggaggtt gtcgataggt tccaaccaaa cggaaaaccg
     2041 caagttcaac tacacggtca aacacccgaa agaccgacca gttccgggcc tttaatcaag
     2101 aaggagaaac gccgcctgta taggcggcgt ttcttatttc cttaacatcc ggcgttggga
     2161 agattcgcgc attttccgtt tttttgcagc a
//
LOCUS       DAOTON010000499         2187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_39002_length_2187_cov_3.497655, whole genome shotgun sequence.
ACCESSION   DAOTON010000499 DAOTON010000000
VERSION     DAOTON010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39002_length_2187_cov_3.497655"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..1055
                     /gene="bamD"
                     /locus_tag="P5292_09635"
     CDS             234..1055
                     /gene="bamD"
                     /locus_tag="P5292_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRS39428.1"
                     /translation="MRLKKLLFLLIPAVFAACSPYNRVLKGSDMDAKMEYAVKLYNKG
                     DYFKALPLLEELITVYRGTQKAERTYYYYAYTNYRLGDYTSAAYDFENFSKTFPTSQF
                     AEECAYMHAYCFYQDSPDFSLDQTNTYKAINELQLFADRYPSSQRITECNRLIDQLRG
                     KLETESFEQARLYFNMEDYKAAVTAFRNLLNDFPSTSFRENALFLIVKSEFLLAENSI
                     DEKKSERYSETLGFYGEFTAAYPESKFKKEADDIAGTTRRRLDRMNGTLNTSSSN"
     gene            1069..1401
                     /locus_tag="P5292_09640"
     CDS             1069..1401
                     /locus_tag="P5292_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRS39429.1"
                     /translation="MNYKADASTTITRNLREYDRETGNIYESIVVIAKRANQISSDLK
                     EELNSKLSEFNSASDNLEEVFENREQIEISKYYERLPKPTLISLSEFNEKKIYHRNPA
                     REENQAGF"
     gene            1475..>2187
                     /gene="coaBC"
                     /locus_tag="P5292_09645"
     CDS             1475..>2187
                     /gene="coaBC"
                     /locus_tag="P5292_09645"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRS39430.1"
                     /translation="MKGARILLGVTGSIAAYKSATLVRLLVKAGADVRVVMTDAATDF
                     IAPLTLSTLSKNPVHRESFDANSGAWDNHVDLGMWADVFVIAPASANTLAKLANGYCD
                     NLLTAVYLSSRCPVWVAPAMDLDMWKHDSTRSNLEKLQAMGVKLIAPAHGELASGLVG
                     EGRMEEPEVILSALQTWWATGRPLLGMKALVTAGPTYEPIDPVRFIGNHSSGKMGFAI
                     AGELAKSGATVTLVAGPVS"
BASE COUNT          550 a          589 c          555 g          493 t
ORIGIN      
        1 atcctggacc tccagggtaa gttgatgagc aacctgactg gtctggatct tgaaaaaggc
       61 ctgatcaact ggggagagca ggtgagcccg ggttgttacc tactggaagc aaaacaggcg
      121 gatggcagga cggagtaccg gaaactgatt cgcaattgac atccagacag gccaggaatt
      181 gtccagatcc atgattttta atgatttgga taatttctta cctttgcgcc ccgatgcgcc
      241 ttaagaagct tctttttctc ctgattccgg ccgtttttgc cgcttgtagt ccatataacc
      301 gggtcctgaa agggtccgat atggatgcca agatggaata tgccgtgaag ctctataaca
      361 aaggcgacta cttcaaggcg ctgccgttgc tcgaggagtt gattacggtc tatcgcggaa
      421 cgcagaaagc cgaacgtacc tactattatt acgcgtacac gaattaccgg cttggggatt
      481 atacgtcggc cgcgtatgat ttcgagaact tctccaagac atttccgaca tcgcagtttg
      541 cagaagagtg cgcttacatg catgcgtatt gcttctatca ggattctccc gatttttccc
      601 ttgaccagac caatacctac aaggcgatca atgaactcca attgtttgcg gaccggtatc
      661 cttccagtca gcggatcacc gaatgcaatc gtttgatcga tcagttgagg ggaaaactgg
      721 aaaccgaatc gttcgaacag gcccgtctct atttcaacat ggaggactac aaagccgcgg
      781 ttacggcttt ccggaatctg ctcaacgatt ttccgtcaac ttcctttcgg gaaaatgccc
      841 tctttctgat cgttaaatcc gaattcttgc tggccgaaaa cagcattgac gagaaaaaat
      901 cggaacgtta tagcgaaacc ctggggttct atggagaatt caccgcagcc taccccgaaa
      961 gtaaattcaa gaaagaggcc gacgatatcg ccggtaccac ccgtcgccgc cttgaccgca
     1021 tgaacggtac cctcaataca tccagcagca actaatttcc tcgagcccat gaattacaaa
     1081 gcagacgcat cgacgacgat cacccgtaac ttacgcgagt acgatcgtga gaccggaaac
     1141 atctacgaat ccatcgttgt catcgccaag cgcgccaacc agatctcttc ggaccttaaa
     1201 gaagaactga actccaagct ttccgaattc aacagcgcgt cggataacct ggaagaagtg
     1261 ttcgaaaacc gcgagcagat cgagatctcc aaatattatg agcgactgcc taaaccgacg
     1321 ctcatctcct tgagcgaatt caacgagaag aagatctatc accgtaatcc tgcccgggaa
     1381 gaaaatcagg cgggattcta agtcctggca ttcaaagtat ccagtgaaaa gaacccggtg
     1441 acttcatcgg gttcttttcg tctttaagcc aagtatgaaa ggtgcccgta tactccttgg
     1501 cgtaaccggc agcattgccg cttacaaatc cgccacgctg gttcgtctgc tcgtaaaggc
     1561 aggcgcggat gttcgcgtgg tcatgactga cgcggccacc gatttcattg ccccgctgac
     1621 actctccacc ctcagtaaaa acccggtaca ccgggagtcc tttgatgcca actccggcgc
     1681 ctgggacaat catgtagacc tcggcatgtg ggccgatgtt ttcgtaatcg cgcccgcttc
     1741 ggccaataca ctggccaaac tggcgaacgg ttattgtgat aacctgttga ctgccgtcta
     1801 cctctcgtcg cgttgtccgg tctgggtcgc gccggcgatg gacctcgaca tgtggaaaca
     1861 cgacagcacc cgctcgaacc tggaaaagtt gcaggcgatg ggagtaaagc tcatcgcccc
     1921 ggcgcacggc gaactggctt ccggactcgt aggcgaagga cgaatggaag aaccggaagt
     1981 gatcctgtcc gcactgcaaa catggtgggc gaccggacgt ccgctgctgg gcatgaaagc
     2041 gctagtcacc gcgggtccaa cctatgaacc gatcgatccg gtccggttca tcgggaatca
     2101 ctcctcaggc aagatggggt ttgcgattgc cggcgaactg gcaaaatcgg gagcgacggt
     2161 aactctggtc gccggtccgg tcagttt
//
LOCUS       DAOTON010000500         2182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_39114_length_2182_cov_3.535026, whole genome shotgun sequence.
ACCESSION   DAOTON010000500 DAOTON010000000
VERSION     DAOTON010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39114_length_2182_cov_3.535026"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="P5292_09650"
     CDS             complement(<1..287)
                     /locus_tag="P5292_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39431.1"
                     /translation="METIKFLLDFILHINDHLDALVLQYGSTIYAILFLIIFVETGLV
                     IMPFLPGDSLLFAAGALCARTADQPDAPLQIGFLIGLLFIAAVLGDNSNYA"
     gene            416..859
                     /locus_tag="P5292_09655"
     CDS             416..859
                     /locus_tag="P5292_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRS39432.1"
                     /translation="MMAWLIFFHLVGATIWTGGHLILATRILPEALRKRDPAILKAFE
                     EPFEKIGIPALLVQTITGLLLAGHYLNHWWYAFRFETPMHTWIAWKLMFLGATILIAA
                     HARIRLITRLKEENLGYLAAHIIVITLLSIAFLVLGTTLRMGGWS"
     gene            complement(883..>2182)
                     /locus_tag="P5292_09660"
     CDS             complement(883..>2182)
                     /locus_tag="P5292_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS39433.1"
                     /translation="AITLLEAPILALVLGFILRYAPADTDYSMSANPNVPAYLFICVI
                     VALFLGLSVSAEEILRDRKIRRRESFLHLSPLAYLLSKIFILFLISAIQTSTFILVGN
                     LILDIQGMGMTYFGILFAVSCFSNLLGLNISSAFDSAVTVYILIPFLIIPQILLSGVI
                     VRFDKLNPVIGAATSVPVIGDIMASRWAFEALAVAQFRENDFEQPLFASDAAMSRATY
                     RKDWWMPVVRERIDRSERLLKAGKRDKEATQSWQLVREELGPEATRYRLATATFLQGN
                     AGEANMAAIEAARNLLKQLKDVYIRQYNNAEQARERAHAAQVKRFGSDESLEVVRRKG
                     SNESLSDLVRGATVKERVEVVNNRIYQRYEPVFQDPGNKRFFSSPFFIADKPVGSGTS
                     MSTYASNLLVIWSMCLLLFVTLYTDLLRRLLQFFARVSKR"
BASE COUNT          543 a          614 c          583 g          442 t
ORIGIN      
        1 atcgcgtagt tggagttatc ccccaacacc gccgctatga acagtagccc gatcaggaat
       61 ccgatctgta aaggtgcatc cggctgatcg gccgtgcgtg cgcacaacgc ccccgccgcg
      121 aacaacaagg aatcaccggg aagaaacggc atgatcacca gacccgtctc cacgaaaatg
      181 atcaggaaca ggatggcgta gatcgtactg ccatattgaa ggaccaatgc atccagatgg
      241 tcgttgatgt gcaggataaa atccagtaag aatttgatcg tttccaagaa gagccggttg
      301 aatttgcgta aaagtactat tcgggacgca cataacccgg tagccatgac gccggtcaaa
      361 gaagcgccta cggttcggca gaaagaatca gccggcagag caatacattt gcccgatgat
      421 ggcctggttg atcttctttc atctggtcgg tgcaacgatc tggacaggcg gtcacctcat
      481 tcttgcgacg cgtatccttc cggaagcgtt gcggaaacgg gaccccgcga tcctgaaagc
      541 ctttgaagag ccgtttgaaa aaatcggcat accggccttg ctggtgcaaa cgattaccgg
      601 cttattgctt gccggacatt acctgaatca ttggtggtat gcattccggt tcgaaacgcc
      661 gatgcatacc tggatcgcct ggaaactgat gtttctggga gcgacgatcc tgattgccgc
      721 acacgcccgc atacggctga tcacgcgact caaagaagag aacctcggct acctcgcggc
      781 ccacattatc gtgatcaccc tgctgtccat cgcattcctg gtgctgggca caacccttcg
      841 catgggcgga tggagttgat cacccggtcg taaaatgccg gatcaacgtt tggaaactct
      901 tgcaaagaac tgcagcaagc ggcgtaacag gtcggtgtaa agggtaacga acagcaacag
      961 gcacatcgac cagatgacga gcaggttgga cgcataggtc gacatgctcg tgccggatcc
     1021 aaccggctta tcggcaatga agaacggact cgaaaaaaag cgcttgttac ccggatcttg
     1081 aaagaccggc tcataacgct gatagatcct gttgttcacg acttccaccc gctctttcac
     1141 ggtggcaccc cgcaccaggt ccgagaggct ctcgttcgac cctttgcggc ggactacctc
     1201 cagggactca tcgcttccaa atcgttttac ctgggcggcg tgagcacgtt cacgcgcttg
     1261 ttcggcgttg ttgtattgtc ggatgtacac gtccttcaac tgcttcagga gattccgggc
     1321 cgcttcaatg gccgccatgt tcgcttcgcc tgcgttccct tgcaggaaag tcgcggtagc
     1381 cagccggtaa cgcgtggctt caggacccaa ttcctccctg accagttgcc aggactgcgt
     1441 cgcttctttg tcgcgcttgc ccgctttgag caaccgctcg ctgcgatcaa tccgctcccg
     1501 caccaccggc atccaccagt cctttcgata cgtcgcccgg ctcatcgccg cgtcggaagc
     1561 aaacaggggt tgctcgaaat cgttctcccg aaactgcgca accgccagcg cttcaaacgc
     1621 ccagcgggaa gccatgatat cgccgatgac cggtacggaa gtagcggcac caatgaccgg
     1681 attgagttta tcaaaccgca cgatcacgcc gctcagcaag atttgcggaa tgatgagaaa
     1741 cggaatcaga atgtaaacgg tcacggcgga atcgaacgcc gaggagatat tcaaccccag
     1801 gagattggaa aaacaggaaa ccgcgaaaag tatcccgaag tacgtcatgc ccatcccctg
     1861 aatatccagg ataagattgc cgaccaggat gaacgtagat gtctgaatag ccgagatgag
     1921 gaacagaatg aagatcttcg acaacaggta ggccaaggga ctcaaatgaa ggaatgattc
     1981 ccgcctgcgg atcttccggt cacgcagtat ttcttcagca ctgaccgata atcccaggaa
     2041 caaagccacg atgacacaga tgaacaggta cgccggtacg ttcggattgg ccgacatact
     2101 gtagtccgta tcggccggcg cataccggag tatgaacccg agtaccaggg ccagaatagg
     2161 cgcttccagc agggtgatgg cc
//
LOCUS       DAOTON010000501         2178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_39204_length_2178_cov_3.035327, whole genome shotgun sequence.
ACCESSION   DAOTON010000501 DAOTON010000000
VERSION     DAOTON010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39204_length_2178_cov_3.035327"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="P5292_09665"
     CDS             <1..434
                     /locus_tag="P5292_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HRS39434.1"
                     /translation="DEELFRAWCEGRTGYPIVDAGMRELNATGFMHNRVRMITASFLT
                     KFLLIDWRWGEAYFAAKLLDFELASNNGGWQWSAGTGVDAAPYFRIFNMDEQTRKFDP
                     DFSYIRRWVPEFQELTYPRPIVDYKMARQRCLEFYKVGLEG"
     gene            487..1524
                     /locus_tag="P5292_09670"
     CDS             487..1524
                     /locus_tag="P5292_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39435.1"
                     /translation="MGSHWKYWDYRASAAYFITINSNRRIEWFGKNEGGSIRFTPVGE
                     IAERFWREIPVHFPNVALGPFVVMPDHLHGIIILGSNDFFNGDREGVVSRRANMLPTI
                     PEELWKIKEDHSVGRFSILDSGPIINPPGGRLIEGPLNEYSINPPSGRSIERPLGGLM
                     ECQDKTTDSQLTNMEIDRAPAEQLLPQQFDEWMAMRYKLEKGVEASFHSMVDRTYRYS
                     PDTPVVENPFFVPYPLVQGRLNLGRFYFDQETRLNTPPFMDHFSARSEEELNIDLQTS
                     YMSMISPLKGSLSLIIRQYKAAVSKHVRPFSPLFRWQKRFFVKIITDPVAYENIARYI
                     RENPRVASKSR"
     gene            1596..>2178
                     /locus_tag="P5292_09675"
     CDS             1596..>2178
                     /locus_tag="P5292_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRS39436.1"
                     /translation="MKKTLLLLFLLAAGVTRPLQAQIAPGAIAPNIVATDINGNNWNL
                     YDLLDQGKTVFLDVFATWCGPCWSYHNSGALDDFYNQYGPDGTNEIMAFMIEGDGSTT
                     MADLQGTGGNTVGNWITGTPMPIIDNSGIASDYQIRYFPTIFMICPDRIVREVGQLST
                     VDLEAVRAECSIATQVNDAGITNSMMYLNGTPAG"
BASE COUNT          520 a          551 c          550 g          557 t
ORIGIN      
        1 acgacgagga actgttcagg gcctggtgtg agggtcggac cggttatccg atcgtcgacg
       61 cgggcatgcg cgagctcaac gcgaccggct tcatgcacaa ccgggtgcgc atgatcacgg
      121 cgagtttcct gacgaagttc ctgctaatcg actggcgatg gggcgaagcc tacttcgctg
      181 ccaaactcct cgacttcgaa ttggccagca acaacggcgg ctggcagtgg agcgcgggca
      241 ccggtgtcga tgccgcgccc tacttccgca tcttcaacat ggacgaacag acccgcaagt
      301 tcgatcccga cttttcctac atccgccgct gggtccccga gttccaggag ctgacctatc
      361 cgcgtcccat agtcgattac aaaatggcgc ggcagcgatg cctggagttt tataaggtgg
      421 ggttggaggg ttaggttttt gcatttatta aacgagaatc ttctggcgat ctgatacatt
      481 tcgcttgtgg gaagtcattg gaagtattgg gactaccgag cttcggccgc gtacttcatt
      541 acgatcaatt cgaacagacg aattgaatgg ttcggaaaaa atgaaggtgg ttctattcga
      601 ttcactcctg tcggagagat tgctgaacga ttttggaggg agatacccgt gcattttccg
      661 aatgtggcac ttgggccatt cgttgtaatg cccgaccatt tgcatggaat tatcatactt
      721 ggttcgaatg attttttcaa tggggatcgt gaaggagttg tcagtcgaag agccaatatg
      781 ttaccgacta ttcctgagga gttatggaag attaaagaag atcattcagt aggtcgtttt
      841 agtatactgg atagcgggcc aatcattaac ccgcccggtg gtcgtttgat cgaaggcccc
      901 ctaaatgagt attccattaa ccctcccagt ggacgttcga tcgaacgtcc actgggaggg
      961 ttaatggaat gccaggataa aacgacagac tcacaattga cgaatatgga aattgatcga
     1021 gctcctgctg aacaattact acctcagcaa ttcgatgaat ggatggctat gcgatataaa
     1081 ctcgaaaagg gggttgaagc atcatttcat tcaatggtcg atcgcaccta tcggtattct
     1141 ccagatactc ccgtagttga aaaccccttc ttcgttccat atcctctggt gcaagggaga
     1201 ttaaatctcg gcagatttta ttttgatcag gagactaggc tgaacacgcc gcctttcatg
     1261 gatcactttt ccgcacgttc tgaggaggaa ttgaatatag atttacagac cagctatatg
     1321 tcgatgatct ctcctttaaa ggggtctttg tctttgatca ttcgtcaata caaagcagcc
     1381 gtttctaaac atgtccgccc tttttcgcct ttatttcgtt ggcagaagcg gtttttcgtt
     1441 aagatcatca ctgatccggt tgcctatgag aatatcgcac ggtatatacg agagaatcca
     1501 agagtggctt ctaagtcgcg ttgatcctcc gacttcgttt tcctttaaat gaattcatta
     1561 tcttgtccat cccattcacc acatccagta attcgatgaa aaaaaccctc cttctcctct
     1621 tccttctggc tgcaggcgtg accaggccgc ttcaggccca aatcgccccc ggcgccatcg
     1681 ccccgaatat cgtggcgacc gatatcaatg gcaataactg gaacctctac gacctgctcg
     1741 atcagggtaa gacggttttt ctcgacgtgt tcgctacctg gtgcggcccg tgttggtcgt
     1801 atcacaactc cggcgcgctg gatgattttt acaatcagta cggtccggat ggcaccaacg
     1861 aaatcatggc gttcatgatc gaaggtgatg gtagcacgac catggccgac ttgcaaggca
     1921 ccggtggtaa caccgtaggc aactggatca ccggtacacc gatgccgatc atcgacaaca
     1981 gcggcatcgc atccgattac cagatccgat acttcccgac gatcttcatg atctgccccg
     2041 accgcatcgt gcgtgaagtg ggccagttga gcacggtcga tctggaagcc gtacgtgccg
     2101 aatgttcgat cgcgacacag gtcaacgacg ccggtatcac caacagcatg atgtacctga
     2161 acggtacacc ggccggct
//
LOCUS       DAOTON010000502         2176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_39270_length_2176_cov_2.579444, whole genome shotgun sequence.
ACCESSION   DAOTON010000502 DAOTON010000000
VERSION     DAOTON010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39270_length_2176_cov_2.579444"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2176)
                     /locus_tag="P5292_09680"
     CDS             complement(<1..>2176)
                     /locus_tag="P5292_09680"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS39437.1"
                     /translation="NVQFNNTSTGAVSYYWDLGNGNTSTLANPSNTYTVPGTYTIRLV
                     AIDGAGNRDTLTRVNYITVVGQPSADFSSSVRSSCLDNNSYSFSNLSTGAVSYLWDFG
                     DGTTSTQSNPTHSYAMSGVFTVTLIATNSFGCQEVEIKNTYITIFPKPDATVQATFTS
                     SCDPATAFQFSNNAQATSWLWNFGDGTTANQQNPSHVFSTPGTYNVSLMATNAFGCSD
                     TSDVPAEINVGVANWANFSVAYDTGCAPFTANFSNPNTNLLSCHWDFGDGTTSTDVTP
                     SHIYVNPGTYTVTLIVTTNSGCVDTVRKVNYILVGAKPNVNFSYNITNGCAPLSVQFT
                     NLSSGYDSCQWIFGDGTRSNDISPLHTYTASGVFSVTLKCWSPSGCSKSVQLDSIISV
                     TSTRALLNASPRVGCPPLSVNFNALSPGSGLNYFWSFGDGTTSTQQNPTHNYTTSGSF
                     DVMLVVADSLGCSDTLRRPAYIQTTNPAANYVPPPTTVGCAPLTTQFTDGTVGANGWA
                     WDFGDGSTSTQQNPVHTYSTPGFYTVSLTTTTANGGCEQTISNFSTFDVRGGYAGFTH
                     SVTTCPPYEAQFTDTSSNAVSWLWDFGDGTTSTDQHPNHTFASPGYHSVSLTITTADG
                     CTYTTMQSNGVYFTPFGANFYGIPQDTVFPMPVQFYANSVGATQWLWDFGDGSTSTLE
                     NPFHTYQSNGNYNVTLWISNGVCTLFYDPPPFNFGEPDTTPVD"
BASE COUNT          476 a          501 c          718 g          481 t
ORIGIN      
        1 atccaccggt gtggtatcgg gctcaccgaa gttgaagggt ggcggatcat agaataacgt
       61 acagacgccg ttgctgatcc agagtgttac gttgtaattg ccgtttgact gataagtatg
      121 gaacggattc tccagcgtag aggtagagcc gtcgccgaag tcccacaacc attgtgtggc
      181 accaacggag ttggcataga attgtaccgg catcgggaac accgtgtctt gcgggatgcc
      241 gtagaagttg gctccgaagg gtgtgaagta cacgccattc gattgcatgg tggtgtacgt
      301 gcatccgtct gcagtggtga tcgtcaatga aacactgtgg taacccgggc tggcgaaggt
      361 gtgattcgga tgttggtccg tcgacgtggt gccatcgccg aaatcccaca accaggaaac
      421 agcgttgctc gaggtatcag tgaattgcgc ttcgtaggga ggacaggtgg ttaccgaatg
      481 cgtgaatccc gcatagcctc ctctgacatc gaaggtggag aaattcgaaa tggtctgttc
      541 gcaaccgccg ttggctgtag tggtcgtaag actgaccgta taaaagccgg gagtagagta
      601 ggtgtgtacc gggttttgtt gcgtggaggt ggagccgtca ccgaaatccc aggcccagcc
      661 gttcgctccg actgttccgt cggtgaactg ggtggtgagc ggcgcgcatc cgacggtggt
      721 aggcggaggt acgtagttgg cggccgggtt cgtcgtctgg atgtatgccg ggcggcgcag
      781 ggtgtcggag catccgagcg agtcggccac gaccagcatg acatcgaatg aaccggaggt
      841 ggtgtaattg tgtgttggat tctgttgtgt ggaagtcgta ccgtcgccga acgaccagaa
      901 gtagttcagc ccgctgcccg gagacagggc attgaagttg acggaaagtg gcggacaacc
      961 gacgcgcgga ctggcattga gcagcgcgcg tgtggaggtg acggaaatga tgctgtccaa
     1021 ctggacactc tttgagcagc cgctcggact ccagcatttc agcgtgacgg agaatacgcc
     1081 ggaagccgtg taggtatgca ggggactgat gtcgttggac ctggtgccgt caccgaagat
     1141 ccactgacaa ctgtcgtatc ccgaagacag gttggtgaat tgaaccgaga gcggagcaca
     1201 gccattcgtg atgttgtagg agaagttcac gttaggcttc gcgccgacaa ggatgtagtt
     1261 cacttttcgt accgtatcca cacaaccgga gttcgtcgtg acgataagtg ttaccgtata
     1321 ggttccggga ttgacgtaga tatgtgaagg tgtaacatcg gtggaggtgg taccgtcacc
     1381 gaaatcccaa tgacagctca gcaagttggt attcgggttg gagaagttcg cagtgaaggg
     1441 agcgcaaccg gtatcatagg caacgctgaa gttcgcccag ttggcgacac cgacattgat
     1501 ttcggccgga acatcggagg tatcgctgca tccgaatgcg ttcgtggcca tgaggcttac
     1561 gttgtaagtg cccggggtcg agaaaacgtg cgaaggattt tgttgattgg ctgtagttcc
     1621 gtcgccgaag ttccagagcc agctggtggc ttgggcgttg ttggaaaact ggaaagccgt
     1681 agccggatca caactggaag taaaggtggc ctggacggtg gcatccggct tggggaagat
     1741 cgtgatgtag gtgtttttga tttccacctc ctggcaacca aagctgttgg tcgcaatcag
     1801 ggtcaccgta aagacacccg acatcgcata gctgtgtgtc ggattgcttt gggtggacgt
     1861 ggtaccgtcg ccgaagtccc agaggtaact gacagcgccg gtcgaaaggt tgctaaagga
     1921 atagctgttg ttgtcgagac aggaagaacg taccgacgag gagaagtcgg ccgagggctg
     1981 acctaccacc gtgatatagt tcacgcgggt cagcgtatcg cggttgccgg caccatcgat
     2041 cgccacgagg cggatcgtgt acgtgcccgg aacggtatac gtattcgacg gattcgccag
     2101 ggtcgacgtg ttgccgttgc cgaggtccca gtagtaggac acggcgccgg tcgacgtgtt
     2161 gttgaactgt acgttc
//
LOCUS       DAOTON010000503         2174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_39321_length_2174_cov_2.621992, whole genome shotgun sequence.
ACCESSION   DAOTON010000503 DAOTON010000000
VERSION     DAOTON010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39321_length_2174_cov_2.621992"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="P5292_09685"
     CDS             complement(<1..955)
                     /locus_tag="P5292_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39438.1"
                     /translation="MDYRHFHELLAGKAAPQAGALQELLRDHPYCQAGHLLLVKAFHD
                     QQHIRYEPALRTAAAHQPDRRVLYRIVHDQVQASSDSVFREPVTAAPESPFLEAAKEE
                     SPLLIGAQAVDSTIPAGIFSDHYVPEQVTPEPMSEQPPVLPEPSREAIINPHIQEEPI
                     SLAPVVPIENDDPREVLRRRLAELLGDSASTATVRETKVIPEEPAAPTILNDTPRTTD
                     DFLITAPAGMPEEPIEEAESGITKVTDAKPAEPVKSEDIVDAESEALEDPVFREGLSY
                     ALEETLLHELERLPELSVSPEESHDTAKSPETETTEPKAVSG"
     gene            complement(955..1476)
                     /locus_tag="P5292_09690"
     CDS             complement(955..1476)
                     /locus_tag="P5292_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HRS39439.1"
                     /translation="MRTHAYRSFTLSFLLATLMLLGGCKVNYSLSGASIPPDVKTITV
                     KFFPKTAPLGPANLSQTFTERLKEKFIGQTGLTVVDRNGDLQLEGAITGYSVAPQAIL
                     PNETAAKNRLTITVSVKYTNTKDEKQSFESSFSRFTDYSSSVNLAAAEDELVREVFDQ
                     LVDDIFNKAVINW"
     gene            complement(1548..>2174)
                     /locus_tag="P5292_09695"
     CDS             complement(1548..>2174)
                     /locus_tag="P5292_09695"
                     /inference="COORDINATES: protein motif:HMM:NF036547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRS39440.1"
                     /translation="VPALALTPDAVQVLTEYRWPGNIRQLKNITEQLSILEKSREING
                     ETLSRYLPHVPASSVPMLLRDEAKSQEAFTERELLYKVLFDMKKDMNDMKLLINEIMQ
                     GSGNDSTWKERHEQVINRLRPEPVHSILHTHEPSVTIQPASPQPVVTVSPIQDHEELE
                     ESLSLEDKEKEFIRKALQKHKGRRKLAAKELGISERTLYRKMNEYGIH"
BASE COUNT          408 a          584 c          632 g          550 t
ORIGIN      
        1 tcccgcttac cgctttaggt tcagttgttt cggtttccgg actcttggca gtgtcgtgtg
       61 attcctcagg acttactgaa agttccggta atcgctccag ctcgtgcagc agcgtttctt
      121 ccaaagcgta cgacagtcct tcgcggaata ccggatcttc aagcgcttca ctttccgcat
      181 ccacaatgtc ttccgacttg acaggttccg ccggctttgc atctgttact ttggtgattc
      241 cgctttccgc ttcttcgatg ggttcttccg gcataccggc aggcgcggta atgagaaaat
      301 cgtcagtagt ccgtggcgtg tcgttcagga ttgttggcgc agcaggctct tcggggatga
      361 cctttgtttc cctgacagta gcggttgaag cggaatctcc cagcaactcc gccaggcgac
      421 gacgcaagac ctcacgaggg tcgtcgtttt caatcgggac aacaggtgcg agactgatcg
      481 gctcttcctg aatgtgcgga ttgatgatcg cctcgcggga gggctccgga aggaccggtg
      541 gttgttccga catcggttca ggtgtcacct gctccggtac atagtgatcg gagaaaattc
      601 ctgccggaat ggtgctgtcc actgcttgcg caccaatgag caggggagac tcttctttcg
      661 ctgcctcgag gaacggggac tcaggggccg cggtaacggg ctcgcgaaat acactgtcgg
      721 agctcgcctg tacctgatcg tgtacgatcc ggtaaagtac ccgacggtcc ggctgatgcg
      781 cggcggctgt tcgcagtgcg ggctcgtagc ggatgtgttg ttggtcgtga aaggctttga
      841 cgagcagcag atgacctgcc tggcaatacg gatgatcgcg cagcaattcc tgcaaagcac
      901 ccgcttgcgg tgccgccttg ccggccaata gctcgtggaa atgccgataa tccattacca
      961 gttgatcacc gctttgttga agatgtcatc gaccagctga tcgaagacct cacggaccaa
     1021 ttcatcttcc gccgcggcta agttaaccga gctgctgtaa tcggtgaaac gcgaaaaaga
     1081 ggattcaaaa ctctgtttct cgtctttcgt attggtgtac ttcacactca cggtgatcgt
     1141 aagacggttc ttggcggccg tttcgtttgg taggatcgcc tgtggggcca cggaatatcc
     1201 cgtgatcgcc ccttccagtt gcaggtcgcc gttgcggtcc acgactgtca gcccggtttg
     1261 cccgatgaat ttttctttca atcgttcggt aaaggtctga ctgaggttgg cgggaccaag
     1321 cggagccgtt ttaggaaaga acttgaccgt gatcgtcttc acatccggcg gaatggacgc
     1381 gccggaaagc gagtagttca ccttgcatcc gcccagcaac atcagcgtgg cgagcaggaa
     1441 gctcagggtg aacgatcggt acgcgtgggt ccgcatggtg ttgggaataa cggcggcaaa
     1501 ttccctaacg cgccgttgat acgcaaattt taattcggtc atccgggtca atggattccg
     1561 tattcgttca tcttcctgta cagcgtgcgc tcggagatgc cgagttcttt cgccgcgagt
     1621 ttccgtcggc ctttgtgctt ctgaagcgcc ttccgaatga attctttctc tttgtcttcg
     1681 agtgaaagcg attcttccag ctcttcgtgg tcctggatcg gcgatacggt aacgaccggt
     1741 tgtggtgaag ccggctgaat cgtcacggat ggttcatgcg tgtgcaggat cgaatgcacc
     1801 ggttcgggtc ggaggcggtt gatgacctgc tcgtgtcgtt ccttccaggt ggaatcgttg
     1861 ccactgccct gcatgatctc gttgatgagc agtttcatgt cgttcatgtc cttcttcatg
     1921 tcgaagagga ccttgtacaa caactcgcgc tcggtaaagg cttcctggct cttggcttcg
     1981 tcgcgcaaca gcatgggcac agaagaagcg ggcacgtgag gcaggtaacg cgacagggtc
     2041 tcgccgttaa tctcccggct cttttccagg atggacaact gctcggtgat attcttcagt
     2101 tgccggatat tccccggcca gcggtattcg gtcaggacct gcacggcatc cggcgtcagg
     2161 gccagcgccg gcac
//
LOCUS       DAOTON010000504         2173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_39353_length_2173_cov_2.852691, whole genome shotgun sequence.
ACCESSION   DAOTON010000504 DAOTON010000000
VERSION     DAOTON010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39353_length_2173_cov_2.852691"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /gene="thyA"
                     /locus_tag="P5292_09700"
     CDS             <1..366
                     /gene="thyA"
                     /locus_tag="P5292_09700"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRS39441.1"
                     /translation="PPCHLLFQFYVANGRLSCQLYQRSADSFLGVPFNIASYAALVHM
                     VAQVCDLQVGDFVHTLGDAHIYTNHFEQVRLQLSREPRPLPTLRLNPEVKDIFAFRFE
                     DLVIENYDPHPHIKAAVAV"
     gene            380..868
                     /locus_tag="P5292_09705"
     CDS             380..868
                     /locus_tag="P5292_09705"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRS39442.1"
                     /translation="MILSIIVAASENGVIGTGNKLPWRLSGDLQRVKAITMGHHLIMG
                     RKTYESIGRPLPGRTTVIISRSRDLQVPGCIVVGSLDKAIEVSQTDSEAFIFGGGEIF
                     RAALPVTDRIYYTRVHCQVTGDTHFPDLDPDQWQIIREERFPADEKNEYPCTFTLFER
                     KK"
     gene            complement(924..>2173)
                     /locus_tag="P5292_09710"
     CDS             complement(924..>2173)
                     /locus_tag="P5292_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRS39443.1"
                     /translation="ALALFAAYVTFTGIQGINIFDAKVLAALFIGGMVPVVFSALAMN
                     SVGKAAMDMVNEVRRQFREIPGILEGTGKPEYGRCVQISTAAALREMMLPGIITIVTP
                     IIIGLVMGPEALGAYMAGVTVSGVLWAIFQNNAGGAWDNAKKSFEKGVEINGQTYYKK
                     SEPHKAAVVGDTVGDPFKDTSGPSMNILIKLSSLVGLTIAPLIAVNGGGHGAGMGDDC
                     CKAKTECHGEMKSCDGMQSECAMDSLGNCVIDSTTCAQWMAEGKLDSTDCVMTENGKC
                     HVRQAACMSVCKSNGSGCHGEAKACDDACKKRCEEKGIDCGNGKACPEHQAACDEACM
                     KACKEKGMDCGHGKACPAKGGHHDCASGCDAACMKNCEQKGMNCYGGGQCSEACMKAC
                     EAKGIKCGSGTPCPEKKSDCCKK"
BASE COUNT          451 a          622 c          565 g          535 t
ORIGIN      
        1 cccccctgcc atctgctgtt tcagttttac gtcgccaacg gtcgcttatc ctgtcagctt
       61 tatcaacgca gtgcggattc cttcctgggt gttccgttca acatcgcttc ctacgccgcc
      121 cttgttcata tggtcgcgca ggtttgcgat ctgcaggtag gtgattttgt ccatacactg
      181 ggtgacgccc atatctatac gaatcacttt gaacaagtcc ggctccaact cagccgtgag
      241 ccgcgtccct taccgacgct ccggctcaac cccgaggtga aagatatctt cgcgttccgt
      301 tttgaagacc tggtgatcga aaactacgat cctcatcctc acatcaaagc ggcggtagcc
      361 gtttgatcct gttgtcacga tgatcctatc catcatagtt gcagcctcgg agaatggcgt
      421 gatcggtacc gggaacaagt tgccctggcg gttatcgggt gaccttcagc gcgtgaaagc
      481 catcaccatg ggacatcacc tcatcatggg cagaaaaacc tatgagtcca ttggtcggcc
      541 tttaccgggc cgtacaacgg tgattatcag tcgtagcagg gatttacagg ttccgggttg
      601 tatcgtggtg ggttcactgg acaaggcgat cgaggtgagt cagacagatt cggaggcgtt
      661 catttttggc ggaggcgaga tattccgggc cgctttgcct gtcacggatc ggatctacta
      721 tacacgcgtt cattgtcagg ttaccggcga tacccatttt cccgatctgg atcccgacca
      781 gtggcagatc atcagggaag agcgttttcc ggcggacgaa aagaacgagt atccctgtac
      841 ctttacgctc ttcgaacgaa agaagtgatt gcaaaaaaaa tgtccgccat tggcggacat
      901 ttttttttga tgctggctgg aagttacttc ttgcagcaat cggatttctt ttccggacaa
      961 ggcgttccgc ttccgcattt gatgcctttg gcttcgcagg ctttcatgca ggcttcgctg
     1021 cattggccgc cgccatagca attcatgcct ttttgttcgc agttcttcat gcaggcggca
     1081 tcgcaaccgg atgcgcaatc gtggtggcca cctttcgccg gacaagcttt tccgtgtccg
     1141 caatccatgc ctttttcttt gcaggccttc atgcaagcct cgtcgcaggc agcttgatgc
     1201 tcagggcatg ctttgccgtt tccgcaatcg atgccttttt cttcgcaacg ttttttacaa
     1261 gcatcatcgc aggccttggc ttcgccgtga caaccggaac cgttcgattt gcaaacgctc
     1321 atacaagcgg cctgacggac gtggcacttg ccgttctccg tcatgacaca atccgtgctg
     1381 tcgagtttgc cttccgccat ccactgcgca caggtcgtgg aatcgatcac gcagttgccc
     1441 agggaatcca tcgcgcactc cgactgcatg ccgtcgcagg atttcatctc accgtgacat
     1501 tcggttttcg ccttgcagca atcgtcgccc ataccggcgc catgaccgcc gccgtttacg
     1561 gcaatcagcg gcgcgatggt taagccgacc agtgaactca gcttgatcag gatgttcatc
     1621 gaagggccgg aagtatcctt gaaaggatca ccaaccgtat caccgactac agccgctttg
     1681 tggggctcgg atttcttgta ataagtctgg ccgttgatct caacaccttt ttcaaacgac
     1741 ttcttggcat tgtcccaggc gccgccggca ttgttctgga aaatagccca aaggacacca
     1801 ctcaccgtta caccggccat ataagcgccc agcgcttcag gacccatgac gaggccgatg
     1861 atgatcggcg tgacaatggt gatgataccc ggaagcatca tctcacgaag agcggcggcg
     1921 gtggagatct ggacgcaacg accatactcc ggctttccgg ttccttcgag gatgcccggg
     1981 atctcacgga actggcgacg aacttcgttc accatgtcca tggcagcttt gccaaccgag
     2041 ttcatggcaa gcgcggagaa gacgactggc accatgcctc cgatgaagag cgcggcgagc
     2101 accttcgcgt cgaaaatgtt gatgccctgg atgcccgtaa aggtcacata ggcggcgaac
     2161 agcgcaagag ccg
//
LOCUS       DAOTON010000505         2168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_39480_length_2168_cov_2.737814, whole genome shotgun sequence.
ACCESSION   DAOTON010000505 DAOTON010000000
VERSION     DAOTON010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39480_length_2168_cov_2.737814"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="P5292_09715"
     CDS             <1..656
                     /locus_tag="P5292_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRS39444.1"
                     /translation="PVGATSGGITLTDGSTGCAGVSPLFNLKQVCGSNVQLTLFLQGY
                     YASGGMIPSLFNSGLSANPDDCDSVTVCLMDPVTYSEVECAQGILQSDGSLSLSFAAS
                     GSYFLKVMHRNTLQTWSAAPVSIAGSVSYDFSADNNLSYGPNMTEVEPGIWALWSGDI
                     NQDELIEASDYALVENDVAAFQFGYVVTDLNGDGLVEATDYSRIENNMPMFLFAAHP"
     gene            complement(727..>2168)
                     /locus_tag="P5292_09720"
     CDS             complement(727..>2168)
                     /locus_tag="P5292_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRS39445.1"
                     /translation="ERDELTGRKKHFQQELNRAVQEFASLHHKTEHLQAGIDNNQKLL
                     DNLNAQLHEIDNTLQQDNLSYQEHILQLRVRFDEVTSHLSALQTEFNTLTDEMNRAGT
                     EYNQHNIHCIQQQNKIAGIIRDHGYKVELEGNLQRNITANVAELDYIQEQIRELLQSS
                     TDFDGQLLELYAEKEELEKSVSLVETEFYRSRGEIDELENRIRQIRHQREQCDQLAQA
                     IKERTTELKIQLNSLKERLSVEFNIDINDILEREPSPDWTEEVLREKVAKQKHQLDTY
                     GPINPMAVEAYNEIQERHTFITTQKDDLIKAKESLLQTIEEIEKTARENFMTAFHSIR
                     ENFIRVFRSLFTEDDDCDLVLLNPDNPTDSDVQIVAKPKGKRPLSISQLSGGEKTLTA
                     TALLFGIYLLKPAPFCIFDEVDAPLDDANIDKFNNIIRKFSKDSQFIIITHNKRTMAA
                     TDIMYGITMVETGVTQVVPVDLTEMEETV"
BASE COUNT          395 a          554 c          652 g          567 t
ORIGIN      
        1 ttccagtcgg cgctacgtcg ggaggcatca ccctgaccga tggttccacc ggttgtgcgg
       61 gagtgagtcc gttgtttaac ctgaaacagg tatgcggttc caatgtgcag ttgacattgt
      121 ttctgcaagg ctactatgca agcggaggaa tgatcccttc actcttcaat tccggactca
      181 gtgcgaaccc cgatgattgc gatagtgtta ctgtttgtct gatggatccg gtgacctatt
      241 cggaagtgga atgtgcgcag ggtattctgc agtcggatgg atcgttgtcg ctcagcttcg
      301 ccgcttcggg cagctatttc ctcaaggtaa tgcaccgcaa tacgctgcag acctggagcg
      361 cagctccggt gagcattgcg gggtcagtca gctacgattt ctctgcggat aataatcttt
      421 cctacggccc gaacatgacg gaagtggaac cgggaatctg ggcgctgtgg agtggcgata
      481 tcaatcagga cgaactgatc gaggcatccg attatgcctt agtcgagaac gatgtggcgg
      541 cgtttcagtt cggctatgta gtgaccgacc tcaatgggga cggattggtc gaagccacgg
      601 attacagccg gatcgagaac aacatgccga tgtttctctt cgccgctcac ccctgattcg
      661 aacaggacct atcgcaaaaa cgcccggcac tgccgggcgt ttttcgttta caaatggcaa
      721 gcagcatcag accgtctctt ccatttccgt cagatccacc ggcacgacct gcgtgacacc
      781 ggtctcgacc atggtgatac cgtacatgat gtcggtggcg gccatggtgc gcttgttgtg
      841 cgtgatgatg atgaactgcg agtccttgga gaacttccgg atgatgttgt tgaacttgtc
      901 gatgttggcg tcgtcgagcg gcgcgtccac ttcgtcaaag atgcagaacg gagccggctt
      961 cagcaggtag atgccgaaca ggagcgcggt cgcggtgagg gtcttttctc cacccgacaa
     1021 ctgggagatc gacaaggggc gcttgccttt cggtttggcc acgatctgga cgtccgagtc
     1081 cgtgggattg tccgggttga gcaggaccag gtcgcaatcg tcgtcctcgg taaacaggga
     1141 acggaaaacg cgaatgaaat tttcccggat ggagtggaag gccgtcatga agttctcgcg
     1201 ggccgtcttc tcgatctctt cgatggtctg caagagtgat tccttggcct tgatcaggtc
     1261 gtccttctgg gtggtaatga aggtgtggcg ttcctggatc tcgttatacg cttcaaccgc
     1321 catggggttg atcggaccgt acgtgtccag ctggtgtttc tgcttggcaa ctttttcccg
     1381 caacacctct tccgtccagt cgggtgaagg ttcgcgttcg aggatgtcgt tgatgtcgat
     1441 gttgaattcg accgacaggc gctccttgag cgagttgagc tggatcttta attcggtcgt
     1501 acgttccttg atggcctgag ccaactggtc gcactgctcg cgctggtgac ggatctgccg
     1561 gatgcggttc tccagttcgt cgatctcgcc gcgcgaacgg tagaattcgg tttcgacgag
     1621 gctgaccgat ttctcgagct cttccttctc ggcatagagt tccagcaatt gtccgtcgaa
     1681 atcggtggac gattggagca actcccggat ctgttcctgg atgtaatcca attcggccac
     1741 attggccgtg atgttgcgtt gcaggttgcc ttccagttcg accttgtacc cgtgatcccg
     1801 gatgatgccg gcgatcttgt tctgctgctg gatgcaatgg atgttgtgct ggttgtattc
     1861 cgtaccggca cggttcatct cgtcggtcag cgtgttgaac tcggtttgca gggccgacag
     1921 atggctggtg acctcatcaa accgcacccg gagctgcagg atgtgctcct ggtagctcag
     1981 gttgtcctgc tgcagggtgt tgtcgatctc gtgcaactgg gcattcaggt tgtcgagcag
     2041 tttctggttg ttgtcgatac cggcttgcag gtgttccgtc ttgtggtgca acgaagcgaa
     2101 ctcctgtacc gcccggttca gctcttgctg gaagtgcttt ttccggccgg tgagttcgtc
     2161 gcgttcgc
//
LOCUS       DAOTON010000506         2162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_39604_length_2162_cov_3.902705, whole genome shotgun sequence.
ACCESSION   DAOTON010000506 DAOTON010000000
VERSION     DAOTON010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39604_length_2162_cov_3.902705"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..949
                     /locus_tag="P5292_09725"
     CDS             32..949
                     /locus_tag="P5292_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39446.1"
                     /translation="MRHYLLFCLLACLGFAVSSCKKDAPPENPYDAIDYSTNETSDPE
                     PDPNSIAGLHKNIFSVRCANPGCHDGTFEPDFRTVESSYATLVYQPVNKFTLDSVKFY
                     SHRVLPNNTADSWLVERLVTPTTEYMPSNSVRLSTADIDHVKNWINAGCPDMDGNLPQ
                     RPNLQPNITGYIAVDGSFNRLDTARLDGNPANPFLVDAGTSMSVYFVALDTADGSAAT
                     DPANYTVKKLLLSTDKNDFSAAISANATLYIGSAQAWMVPVNVSAWPSGTTVYFRIAV
                     NDGQHTTDAVFPRNESLDYYKTYFAFHVR"
     gene            939..1382
                     /locus_tag="P5292_09730"
     CDS             939..1382
                     /locus_tag="P5292_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39447.1"
                     /translation="MSAKQLLPFLLIALLTACKKDENEFSEVPEIEFVSITPSVVDQF
                     TDPVTITLRYKDGDGDLGENNADARNCFITDNRIGIEYEYRIRQLAPDGASIPIQGTL
                     DVQLGGQGITDTAAATQSTSFTVVVRDRAGHQSNAVTTGSITIVR"
BASE COUNT          523 a          672 c          518 g          449 t
ORIGIN      
        1 atactgcctg tgaactgcag gccgcgttcg tatgagacac tacctactct tttgcctgct
       61 cgcctgcctg ggcttcgcgg tctcctcgtg caagaaagac gcgccgccgg agaatcccta
      121 cgacgcgatc gactactcca cgaacgagac gagcgatccg gagcccgatc cgaattccat
      181 cgcgggactg cacaagaaca ttttctccgt ccgttgcgcg aatcccggtt gccacgacgg
      241 caccttcgag cccgacttcc gtacggtcga atcttcctac gccacactcg tctatcaacc
      301 ggtgaacaag ttcacgctgg attccgtgaa gttctattcg caccgagtcc ttcccaacaa
      361 caccgccgat tcctggctcg tcgaacgact cgtcaccccg acgaccgagt atatgccgtc
      421 gaacagcgtg cgcctcagca cggcggatat cgatcacgtg aagaactgga tcaacgccgg
      481 ttgtcccgac atggacggca acctgccgca gcgtccgaac ctgcagccga acatcaccgg
      541 ctacattgcc gtggacggca gtttcaaccg gctcgacacg gcgcgcctcg acggcaatcc
      601 tgccaatccg ttccttgtgg acgccggtac cagcatgagt gtttacttcg tagccctcga
      661 caccgccgac ggttcggccg ctaccgatcc ggccaactac actgtgaaga agttgctgct
      721 ctcgaccgat aagaacgatt tcagtgctgc gatcagcgcg aacgcgaccc tgtacatcgg
      781 ttccgcacag gcctggatgg tgccggtcaa cgtctccgca tggccgtcgg gtacgacagt
      841 ctatttccgt atcgcagtaa acgacggtca acacaccacc gacgcggtat tcccgcgcaa
      901 cgaatcgctc gattattaca agacctattt cgccttccat gtccgctaag cagcttcttc
      961 cctttttgct catcgccctg ctgacggcct gtaagaagga cgagaacgag ttcagcgaag
     1021 tgcccgagat cgaattcgtc agcatcacgc cgagtgtcgt cgatcaattc accgaccccg
     1081 tcaccattac gctgcggtac aaagacggcg acggcgacct tggcgagaac aacgccgacg
     1141 caaggaactg tttcatcacc gacaaccgca tcggcatcga gtacgagtac cgcattcgtc
     1201 aactggcacc cgacggcgcc tccattccga tccagggaac gctggacgta caactcggcg
     1261 gacaaggcat caccgacacg gcagcggcta cgcagtcgac cagcttcacc gtcgtggtgc
     1321 gcgaccgggc cggacatcaa agcaatgccg tgacaaccgg ttcgatcacc atcgtgcgat
     1381 gattttttag tttctccttg gcgcgacaat ttcctgtcgc accggcgatc gtctttatgg
     1441 gttggataat cccggaacgg gaacaatcat tcgcattatt tcaaaactcg gcgcatcccc
     1501 aactcaccgc acaaccatgt attataaact tgaatcagca acaacctctc aaatcattta
     1561 ataatacgac acatccccaa ctgctaacaa ttttcatggt tcgacatttt ctccatctca
     1621 ccggcccgcc cctttatggg gcgcaataat ccttgcaccg gcgatccctt ctccaccacc
     1681 cgaccccgta ggggtcgaat aagaaaaagg taaagcgaga taagtggaat gattgaaaaa
     1741 caccgccatg aattgaaatt tgaattctca attccaccct caccgccaaa ttattcgacc
     1801 cctccggggt cggagatcaa gagagagggt cggatgcaag gattattgcg cccctgccgg
     1861 ggcgggtcag tgagaacgga taggctgttg tttcagaaat atgacttcgg aaaaatgcag
     1921 gagaaaataa agtgaaacac tgatgcaaaa gctggtttac cattgtacat caagtgcagc
     1981 tagatgaaac aactactgct cacaattttc atggctcgat cttttcccca actcaccggc
     2041 ccgccccttc atggggcgca ataatccttg atccggcgat tcctcctcca ccaaccggcc
     2101 ccggaggggc cgaataataa aaaggcgata ggtgtaaata ggtatgttca aatgcaccgc
     2161 ca
//
LOCUS       DAOTON010000507         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_39671_length_2159_cov_3.305608, whole genome shotgun sequence.
ACCESSION   DAOTON010000507 DAOTON010000000
VERSION     DAOTON010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39671_length_2159_cov_3.305608"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..900
                     /locus_tag="P5292_09735"
     CDS             25..900
                     /locus_tag="P5292_09735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS39448.1"
                     /translation="MNDKTLSDTAYEFPCNLCSSTSHRLLFVKKNFPIVKCLSCGLVS
                     TVLPADFDPLSIYDETYFNGGQVDGYGDYESSEIILRAEFRKIVREILKYIPQPEGKK
                     LLELGSAYGYFLSEASAYFTCYGVEVSKAGVQKSEALGLRVFEGALTADVLRETGKVD
                     VVVMLDVIEHLTDPMATLGLAYSALNPGGVMLIVTGDHGSLLSKVMKANWRLMTPPQH
                     TFFFTSKTLPAMVAKAGFTVKSVTAPWKIVPLGLPFYQVGRRTGLRVRALENINNFGI
                     PINLFDTVRVIAVKP"
     gene            1415..>2159
                     /locus_tag="P5292_09740"
     CDS             1415..>2159
                     /locus_tag="P5292_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRS39449.1"
                     /translation="MKNKSEKQPYRTLVKQACLKVQGFEDFYKRLSRKIRIAGKSPST
                     LSNYTRYLAQMALHFECVPTELDTEQVEEYLEMMLDQHDTPSESYFKFVVYSLRFAFK
                     AEGLDYKRFTLPSIKRDKKLPVILSREEMRRLLRAPALLKHRLLIGLLYGCGLRCMEV
                     RSIHIRDLDLDRRLLHVRKGKGRKDRYVPLGDLLCRGVRSYLEAENPQTFLFNGKPEG
                     RKGGDFDSRYSQRGVQWAVQQAAKTAGIQK"
BASE COUNT          508 a          569 c          594 g          488 t
ORIGIN      
        1 aagcgctaag aaaatgacct gtacatgaac gataagacct tgtctgatac agcctacgag
       61 ttcccgtgca acctttgcag ctcgacatcc catcgtttgc tttttgttaa gaagaacttc
      121 ccgatcgtaa aatgtctttc ctgcggcctg gtgtcgacag tgttgcccgc cgatttcgac
      181 ccgctcagca tttacgacga aacctatttc aacggtggtc aggtcgacgg ctatggcgat
      241 tatgaatcct ctgaaatcat cctgcgtgct gagttcagaa agatcgtccg cgagatcctg
      301 aagtacattc cacaacccga agggaaaaaa ctactggaac tgggaagcgc ttacggctat
      361 tttctgagtg aggcctcggc atactttacc tgttatggtg tggaagtcag caaagccggc
      421 gttcagaaat ccgaagcgct tggtttgcgg gtatttgagg gggcgctgac cgcggacgtt
      481 ctccgcgaaa cgggaaaagt ggatgtagtc gtcatgctgg atgtcatcga gcacctgacg
      541 gatccgatgg cgacactcgg gctggcctat tccgccttga atcccggtgg cgtgatgttg
      601 atcgttacgg gcgaccatgg gtccctgctt agcaaggtga tgaaagcaaa ctggcggttg
      661 atgacgcccc cgcagcacac ttttttcttt acctcgaaaa cactaccggc catggtcgcg
      721 aaggcgggtt ttacggtcaa gtcggttaca gcgccctgga agatcgtacc actgggatta
      781 ccgttctatc aggtgggcag aagaaccggc ctacgcgtcc gggcattgga aaacatcaac
      841 aactttggta tcccgatcaa tctgtttgat acggtacggg taatcgcggt gaagccttaa
      901 cgccgcagat cgtatatgat ctgacgatta ctacgacgtc ccgacagacg aatgctgccc
      961 ctttgtcccg cgctgacttt ctgttcactt ctgtaaggtc aaatcgcttg agctgatatt
     1021 ccccacgttg atttcccgct caccggctaa caaatggtga aggcttcagc ctgtctgcga
     1081 tcaaatccgg ttaggtattt catgggaagc ggattattct ctccatataa tcagctacgc
     1141 ctttaaagag atcgaaattc tttaaacagg gagggagggt ttttgcacag tctgacgttt
     1201 tggcgcttgg cgcagtggcg gacttagaag cacgaagttg tcaaacagca caaatgccca
     1261 atggaagcac tgcacctgat ttaaccactt cacccgccat tgagccaaac gcctgttgca
     1321 ctaaaccccg ctttcgcggg gtagcctgta ctacctgagc agaactgttt tcggattgat
     1381 cctcgtagaa ggttttacca caaacattct atgcatgaag aacaaatctg aaaaacagcc
     1441 ctacaggacg ctggtaaaac aagcctgttt aaaagtacag ggattcgagg atttttacaa
     1501 acgcctctcg cgcaagatcc gtattgcggg caagagtccc agtacgctga gcaattacac
     1561 gcgctacctg gcccagatgg ccctgcactt tgagtgcgtg cccacggaac tggacaccga
     1621 acaggtggag gagtacctgg agatgatgct cgatcagcac gacacgcctt cggagtctta
     1681 tttcaagttc gtggtgtaca gccttcgatt cgccttcaag gccgaagggc tggactacaa
     1741 gcggttcacc ctgcccagca tcaagcggga taagaaactg ccggtgatcc tgagccggga
     1801 agagatgcgc cggctcttaa gggcaccggc cttactcaag caccggctgc tcatcggcct
     1861 gctctatggt tgcggcctga ggtgtatgga ggtgcgcagc atccacatcc gggacctgga
     1921 cctggatcgg cgactgctgc atgtgcgcaa gggcaaaggg cgcaaagacc ggtatgtgcc
     1981 cctgggtgac ctgctctgtc gcggggtgcg cagctacctg gaggcggaga acccgcagac
     2041 gttcctgttc aacggcaagc ccgagggaag gaaaggcggc gacttcgaca gccgttactc
     2101 ccagcgcggg gtgcagtggg ccgtgcagca ggcggccaag accgccggta tccaaaagg
//
LOCUS       DAOTON010000508         2158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_39701_length_2158_cov_3.376129, whole genome shotgun sequence.
ACCESSION   DAOTON010000508 DAOTON010000000
VERSION     DAOTON010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39701_length_2158_cov_3.376129"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="P5292_09745"
     CDS             <1..773
                     /locus_tag="P5292_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRS39450.1"
                     /translation="SASSGDAASVNVPAAFVDSVADLHLTPSNNCSLESAAVPIAGIT
                     TDADGDTRNSLSPDIGADEFNGLCITIVNVKAFIQGFYVGGGAMNATVDPVGYPALCD
                     TILVRLASDVYPYTVLFEDTAYLQTDGTLEASFPAAASGNSHYLVLKHRNALETWSAA
                     PVVVSNNMLFDFSTTAAQAYGANQTLLEAGIYGLHSGDVNQDELIESSDYSQMENDVL
                     QFLFGYQPTDITGDGLVESSDYALMENNLLQFIFVIKP"
     gene            complement(813..1289)
                     /locus_tag="P5292_09750"
     CDS             complement(813..1289)
                     /locus_tag="P5292_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39451.1"
                     /translation="MRPATLAEISKELATRSPKQLLDYCIRLAKHKAENKELLTYLVF
                     ESSDEAGYRKHIRDQVSARFGEMNRSTLYLAKKSVRKVLRDLNRYIRYSGKQETEIEL
                     RMHFLQTLRDSGIPYRDSPVLVNLYEGQLKKIRQTIAKLHEDLQFDYEQELSNLSV"
     gene            1415..2116
                     /locus_tag="P5292_09755"
     CDS             1415..2116
                     /locus_tag="P5292_09755"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRS39452.1"
                     /translation="MRLLLPGILCLSLFSSCSLTCTQGEGPVVDDHRKLDRFDRIQLN
                     FDARISIHKGDVPGLVIHAQENLLNEIVAKESGGKLELNSESCMEVDEPVRIEITVPD
                     LRELEVTGSGDIVLLDTFMVETIELSVTGSGSIHGRLVAARIESEVTGSGDIVLEGSA
                     NEQVIDITGSGDVKAMALPCNTADVDVTGSGDAYLYVIKQLDAEVSGSGTVHYKGKPE
                     MKSEVNGSGKVVDDN"
BASE COUNT          482 a          541 c          607 g          528 t
ORIGIN      
        1 agtcggccag tagcggtgac gcagcgtctg tcaatgtacc ggcggccttt gtggatagtg
       61 ttgcggattt gcacctgaca ccgtccaaca attgttcgct ggaaagcgcc gcggtcccca
      121 ttgccggaat cactacggat gcggatggtg atacccgcaa ttctctcagc ccggatatcg
      181 gagcggatga gttcaacgga ctctgtatta cgatcgtcaa tgtgaaggcc tttatccagg
      241 gattctatgt tggcggagga gccatgaatg caacggttga cccggtggga taccctgcct
      301 tgtgcgatac catcctggtc cgactggcca gcgacgttta tccatacacg gtcctgtttg
      361 aggatacagc ttaccttcaa acggatggaa ccctggaagc cagcttcccg gctgcagctt
      421 ccggcaacag ccattacctc gtcttgaaac accggaatgc tttggaaacc tggagcgccg
      481 ctccggtagt ggtgtcaaac aacatgttgt tcgatttctc caccactgct gcacaggcat
      541 acggggcaaa tcaaacactg ctggaagccg gcatttatgg tctgcacagt ggtgacgtaa
      601 accaggatga attgatcgaa tcgagtgatt attctcagat ggaaaacgac gtattgcagt
      661 tcctgtttgg atatcaaccg accgatatta ccggggacgg cctggtcgag tcaagtgatt
      721 atgcgctcat ggaaaacaac ctgttgcagt ttatcttcgt gattaagcca taaactcgtc
      781 ggatagctta ttcgaaagga ttggttgttg cgtcaaacgg aaaggttcga taattcctgc
      841 tcgtagtcga actgcaggtc ttcatgcaac ttcgcgatcg tctgccggat tttcttcaat
      901 tgaccttcgt acaggttgac caatacggga ctatcacggt agggaatgcc ggagtcgcgt
      961 agtgtctgca gaaaatgcat gcgtaactcg atctccgttt cctgctttcc gctgtaccgg
     1021 atgtagcggt taaggtcgcg caggaccttg cggacgcttt tcttggccag gtaaagcgta
     1081 cttctgttca tttccccgaa ccgggcgctg acctggtcgc ggatgtgctt tcggtaaccc
     1141 gcctcatcgg aggattcgaa gacgaggtag gtcagcaatt ccttgttctc cgctttgtgt
     1201 ttcgccagcc ggatgcaata gtccagcaac tgcttgggag atcgggtggc gagctctttg
     1261 gagatttctg ctaaggtggc gggccgcata tcgggttcaa agttagggac gacggattcg
     1321 gtacggatgg tttcatgctc gaaatgctaa cagccttgcg gggtgttgcg gatttacgtc
     1381 ctttgctgta ggtttaacca tgcttcgatc gaccatgcga ctgcttcttc ctggcattct
     1441 ttgcctatcc cttttcagtt cctgtagtct gacctgtacc cagggcgaag gccccgtggt
     1501 cgacgatcat cgaaaactcg atcgctttga tcggatccag ttgaatttcg atgcacggat
     1561 ttcgatccat aaaggtgatg tccccggtct tgtcatccac gcgcaggaga atctgctgaa
     1621 tgaaatcgtg gccaaggaga gcggcggaaa actcgaattg aattcggaat cctgcatgga
     1681 ggtggacgag ccggttcgga tcgaaatcac cgtaccggac ctgcgcgaac tggaagtgac
     1741 cggcagcggt gatatcgtgt tgctggacac gtttatggtc gaaaccattg agctatcggt
     1801 gaccggctcc ggcagcattc acggacgcct tgtcgccgcc cgaatagaaa gcgaagtaac
     1861 cggctccggc gatatcgtgc tggaaggttc cgcgaacgaa caagtgatcg acatcaccgg
     1921 ttccggcgac gtgaaagcga tggccttgcc ctgcaatacc gcggatgtgg acgtgaccgg
     1981 ctccggggat gcttacttgt atgtcatcaa gcaactcgac gctgaagtca gcggtagcgg
     2041 taccgttcac tacaaaggca aacccgaaat gaagtcggaa gtcaacggca gcggtaaagt
     2101 ggtggatgac aattaatgaa ccaggttcag ttcaggtgca cccgcaccgg taacgtat
//
LOCUS       DAOTON010000509         2158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_39706_length_2158_cov_3.120780, whole genome shotgun sequence.
ACCESSION   DAOTON010000509 DAOTON010000000
VERSION     DAOTON010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39706_length_2158_cov_3.120780"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..213
                     /locus_tag="P5292_09760"
                     /pseudo
     CDS             1..213
                     /locus_tag="P5292_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871300.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(282..1205)
                     /locus_tag="P5292_09765"
     CDS             complement(282..1205)
                     /locus_tag="P5292_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRS39453.1"
                     /translation="MATPFKGPVVCLGASLVDLTFRCDLEPVLHTSNPSQMHRSPGGV
                     VRNIAHHLALLGTPVELLTVLGRDPDGYWLERQCRDAGIGMEHIAWAEETTGTFASVA
                     TPAGDLYIGAVTSDTDKLMTIDFMNERAQLIRSASVVIADCNLSVEALRWLMGFCAQH
                     QVPLIVETVSIPKAKRLRDALPGKLLLVKPNREELAVFEKDQHVTPEEKIAHLHASGF
                     RNVWLSRGADGSLFSDGSRMVPIPAPRVHVRDVNGAGDASLAGWVWAWLGDKDPLTCV
                     HYGHAAAACLLEVYGAVRSDLSPALLEQRLP"
     gene            complement(1283..1756)
                     /locus_tag="P5292_09770"
     CDS             complement(1283..1756)
                     /locus_tag="P5292_09770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS39454.1"
                     /translation="MGEIRYLQVGLEQVSWLVDSRVEFLTEYWGAKGQEETQQLRDEL
                     ENFFRRELPAGTFVAWLAMDGDQWVGVGGMKLVDKPGGFRAMNGRSGYIMNMYTRSAW
                     RKRGIATAILELLLEHGRQAGASYFELHATTDGAPVYERAGFVRHGEPTYRKFVQ"
     gene            complement(1856..2149)
                     /locus_tag="P5292_09775"
     CDS             complement(1856..2149)
                     /locus_tag="P5292_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRS39455.1"
                     /translation="MWHRHPVWFLDGNPVVGYSKLKDCIRLLFWSGQSFDEPGLAPEG
                     SFKAAEARYTELKQVKKKDLKRWLGKAREIQWDYKNLVKRRGRLEWLVVGSEQ"
BASE COUNT          450 a          692 c          579 g          437 t
ORIGIN      
        1 ttgcccgcgg acgccatccg cctggtctgc ggccccggcg agacggtggg cgcggcgctg
       61 gtggcgcatc cgcgctgcgc cggcgtgtgc ttcaccggct cgacccaggt cgccaggatc
      121 atccagcgcc agctcgcgat gggcgacggc ccgatcgtgc cactgatcgc cgagaccggc
      181 ggcctgaacg cgatgatcgt ggattcgagc gcgaccaccg ggccgccctg ttggagttgc
      241 tgcgcgactt ccggatgcag gtgaatacgt ggcgtcatga tttacggaag ccgttgttca
      301 aggagtgcag gtgaaaggtc gctgcgtaca gcaccataca cttcgagcag gcaggctgcc
      361 gcggcatggc catagtgaac acaagtcagc gggtctttat ccccgagcca ggcccacacc
      421 cagcctgcca gcgaagcatc gccggcgccg ttcacgtcgc gtacatgtac acgcggtgcc
      481 ggaatcggta ccatacgact gccgtcggag aacagcgacc cgtctgcgcc acgactcagc
      541 catacatttc gaaaacccga tgcatgcagg tgagcgatct tttcctccgg ggtgacgtgt
      601 tggtcttttt cgaagacggc gagttcttcg cggttcggtt ttaccagaag tagttttccg
      661 ggtaaggcat cccgcaggcg tttcgctttg ggaatcgaaa cggtctcgac gatcaacgga
      721 acctggtgct gggcgcagaa tcccatcagc cagcgaagcg cttcgacgct caggttgcag
      781 tcggcgatca ccaccgaagc ggaacggatc aactgcgcac gttcgttcat gaaatcgatg
      841 gtcatcagct tgtcagtatc ggatgtcacc gctccgatgt agagatcgcc ggctggcgtt
      901 gcgactgacg cgaaggttcc ggtcgtctct tccgcccagg cgatgtgttc cataccgata
      961 cccgcgtccc gacactgtcg ttcgagccag tagccgtccg gatcccgccc gagtaccgtg
     1021 agcaattcaa ccggcgttcc cagcaaggcg aggtgatggg cgatgttgcg caccacgccg
     1081 cccgggctcc ggtgcatttg ggaaggattc gaggtatgca ggaccggctc gaggtcgcag
     1141 cggaaggtca ggtcgaccag ggatgcccca agacagacga ccggaccctt gaacggggtt
     1201 gccataacgg ccgtaaatat cgtgtaaaat agggaacacc gaccgttgaa atccccaagg
     1261 aacagcgact ccgcgggttc gcttactgca caaacttccg gtacgtaggt tcgccatgcc
     1321 tgacgaaacc cgcgcgttca tacaccggag caccatcggt ggtcgcatgg agttcgaaat
     1381 aactcgcgcc cgcctgacgg ccgtgttcga gcaacaactc aaggatcgca gtagcaatgc
     1441 cccgtttgcg ccaggcgctt cgggtgtaca tgttcatgat gtagccgctg cggccgttca
     1501 tcgcgcgaaa cccgccgggc ttatcgacca gcttcatgcc gccaacgccc acccattggt
     1561 cgccgtccat agccagccag gccacaaagg tcccggccgg cagttcacgc cgaaagaaat
     1621 tttccagttc gtcccgcagc tgctgcgttt cctcttgacc tttggctccc caatactccg
     1681 tcaaaaactc aacacgcgaa tccaccagcc aagaaacctg ctccaacccg acttgcaaat
     1741 acctgatctc acccatgatt agctaaataa ttcgattacc aataagcacg agcacaaacc
     1801 agcaaccaac cgcacactat tcgctattcg ctattcgcta ttcgctgttc actgtttact
     1861 gttcactgcc cactaccaac cactccaacc tccccctccg cttcacaaga ttcttataat
     1921 cccactgaat ctcccttgcc ttacccagcc agcgcttcag gtcctttttc ttcacctgtt
     1981 taagctcggt ataacgggcc tccgccgctt tgaaactccc ttccggtgcg aggccgggtt
     2041 cgtcgaacga ctgtccgctc caaaataaaa gccgtatgca gtctttcaat ttactataac
     2101 ccacaaccgg attaccgtcc aggaaccaga cgggatgccg gtgccagatc tttccttc
//
LOCUS       DAOTON010000510         2153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_39817_length_2153_cov_3.030505, whole genome shotgun sequence.
ACCESSION   DAOTON010000510 DAOTON010000000
VERSION     DAOTON010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39817_length_2153_cov_3.030505"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1393
                     /locus_tag="P5292_09780"
     CDS             200..1393
                     /locus_tag="P5292_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRS39456.1"
                     /translation="MSLLEPIRNLARKYHGDIIACRRHLHQHPELSFHEFETQRFVET
                     RLTEFGITDFKRMANTGVVALLKGKNADKRTVALRADLDALPIVEANAVDYKSKNEGV
                     MHACGHDVHTASLLGVARILHELRDRFEGTVKLIFQPGEEKLPGGASLMIKEGVLENP
                     KPASVIGQHVMPQIPAGKVGFRKGLYMASTDELYVTVHGKGGHGAMPHLNIDPVMITA
                     QILVALQQIVSRFAKPSLPTVLSFGKVIANGATNVIPDKVCLEGTFRTLNEEWRDEAH
                     RRMKHMAEQIAESMGGRCEFEIRRGYPFLVNDEDLTDRSRRWAEEYVGKENVEDLEIW
                     MAAEDFAFYSQQAKACFYRLGVRNEAKGITSSVHTPTFDIDESALETGMGLMAWLAIR
                     ELEQT"
     gene            complement(1638..>2153)
                     /locus_tag="P5292_09785"
     CDS             complement(1638..>2153)
                     /locus_tag="P5292_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRS39457.1"
                     /translation="YTALGVFRDDSKEGRPLNMDKIRYHKVIIMTDADVDGSHIRTLL
                     LTFFFRHLKELIEAGYLYIATPPLYLVKKGKDSTYAWNDEQRIAAIKQLAGEGKEENV
                     NVQRYKGLGEMNAEQLWETTMNPSTRTLRKVTIESAAEADRIFSMLMGDDVPPRRDFI
                     EKNSKYAKIDA"
BASE COUNT          466 a          608 c          580 g          499 t
ORIGIN      
        1 actcatcagc cagcgtgaac cgatcgtcgg tggcttcggc ttcggcctgc gcagccgcct
       61 gctcggttat ttcatccgcg ccgactgggc ctggggggtc gaagaccagg tggtccagcc
      121 tcgccgcttc tatttctctc tcggactcga tttctgacag cgcctcgtcc ctcgattcac
      181 gtctctcgtc cctcccttca tgtccctgct cgaaccgatc cgcaacctcg cccgcaagta
      241 ccacggagac atcatcgcat gccgccgcca tctgcaccaa catccggagt tgtcctttca
      301 cgaattcgag acgcagcggt ttgtggaaac acgtctgacg gagttcggca tcaccgattt
      361 taagcgcatg gccaacaccg gcgtggtcgc gcttttaaag ggcaagaacg ccgacaagcg
      421 tacggtcgcc ctgcgagccg atctggatgc tttgccgatc gtggaggcga atgcggttga
      481 ctacaagtcg aagaacgaag gggtgatgca cgcctgtggt cacgacgtgc atactgcttc
      541 actgctcggt gtggcacgca tcctgcatga actgcgcgac cgctttgaag gcaccgtgaa
      601 gctcatcttc cagccgggag aagaaaaatt acccggcggc gcgtcactca tgatcaagga
      661 gggcgttttg gagaatccga aacccgcttc ggtcattggt cagcacgtga tgccgcagat
      721 cccggccggc aaagtcggtt tccggaaagg tctctacatg gccagcacgg atgaattgta
      781 tgttaccgta cacggcaaag gcggacatgg ggcaatgccc cacctgaaca tcgatccggt
      841 gatgattacc gcgcagatcc tggttgcctt gcagcagatc gtcagccgct ttgccaagcc
      901 ttcacttccc actgtgcttt cgttcgggaa agtcatcgcc aacggtgcta ccaatgtcat
      961 cccggacaag gtctgcctgg aaggaacgtt ccgtaccctc aacgaggaat ggcgcgacga
     1021 agctcaccgg cgtatgaaac acatggccga acaaatcgcc gaaagcatgg gaggccgttg
     1081 tgagttcgag atccgtcgcg ggtacccctt cctcgtcaac gacgaagacc tgacggatcg
     1141 cagtcgccgt tgggccgagg agtatgtcgg caaggagaat gtggaagatc tcgagatctg
     1201 gatggccgcg gaagacttcg cgttttattc ccagcaagcc aaggcttgtt tctatcgcct
     1261 cggcgtccgg aacgaagcga aaggcattac ttcctccgtt cacaccccaa ccttcgacat
     1321 tgacgaaagc gccctggaaa caggcatggg tctcatggcc tggctggcca tccgcgaact
     1381 ggaacaaacc taaaaaccaa gtcacccaat aaaaaaaggc agcgcgaaac gctgcctttt
     1441 tttatcgcgg aaatgtggtt ggtaactgga aattggtaat tagtgattag tgattagtaa
     1501 ctattgtaaa gcaactctag gcgtttattc tcgaatcgaa ctcctggaaa atcaatagac
     1561 cctaatcccc aataactatt cgctattcac tattcactat tcactgttca ctgttcactg
     1621 ttcactattc actatcctca agcatcaatc ttcgcatact tcgaattctt ctcgatgaaa
     1681 tcccgacgcg gcggaacatc atcgcccatc agcatggaga agatgcggtc ggcttcggcc
     1741 gcgctttcga tcgtcacttt gcgcagggtg cgcgtgcttg gattcatggt ggtctcccac
     1801 aactgctcgg cgttcatttc accgagaccc ttgtagcgtt gtacgtttac gttctcttct
     1861 ttaccctcgc cggcgagttg cttgatggcg gcgatccgct gttcgtcatt ccaggcgtag
     1921 gtagaatcct tgcctttttt gacgaggtag agcggaggcg tagcaatgta gaggtagccg
     1981 gcttcgatca gttccttgag atggcggaag aagaaggtga gcagcagggt gcggatgtgt
     2041 gatccgtcca cgtcggcatc ggtcatgatg atcaccttgt ggtagcggat cttgtccatg
     2101 ttcaacggac gtccttcctt gctgtcgtcg cggaagaccc ctagcgcggt gta
//
LOCUS       DAOTON010000511         2149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_39903_length_2149_cov_2.635148, whole genome shotgun sequence.
ACCESSION   DAOTON010000511 DAOTON010000000
VERSION     DAOTON010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39903_length_2149_cov_2.635148"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1041)
                     /locus_tag="P5292_09790"
     CDS             complement(217..1041)
                     /locus_tag="P5292_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRS39458.1"
                     /translation="MSSPQSVKHGPFNWEYLVFGNGPELLLAFHGFDNNADDFRLFEP
                     LLGQQYTIVAVNLFYHGRSYAEGAPDQLQFRREDLQALITQLLERFPHARFSLLGFSL
                     GGRICLELVSLFAGRVDRLILLAPDGLVISPWYVFVTRTIAGRWLFRRVLRRPQRFLR
                     LGGWLRTLGLVGEKQYKFALSYFGSGPQRELVFKVWMIFRYLLPRKAALSAALKQHPV
                     RTDLFFGRRDSIIRPSFGIIYQKKVDAQANIHILETGHNLLKSDVAERVLAVVSSQ"
     gene            1266..>2149
                     /locus_tag="P5292_09795"
     CDS             1266..>2149
                     /locus_tag="P5292_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRS39459.1"
                     /translation="MPRDTSIHSVLIIGSGPIIIGQACEFDYSGSQASRSLKEEGIEV
                     ILINSNPATIMTDKVTADHIYLKPLTRKSIIEILEKHKIDAVLPTMGGQTALNLAIDC
                     QKAGIWEKYGVRIIGVDIDAIHTTEDREKFRLRMLELNAGVCKGRTATSFLEGKEIAQ
                     EIGFPLVIRPSFTLGGTGGGFVNTREEFDAALNRGLHASPVHEVLIEQSIFGWKEFEL
                     ELLRDAAGNVIIICSIENFDPMGIHTGDSITVAPALTLTDKEYQIMRDASIAVLREIG
                     VDTGGSNVQFAVNPANGR"
BASE COUNT          470 a          593 c          666 g          420 t
ORIGIN      
        1 ggttgccggt gaatagcccc gatggcagcg agctcccgcg agcggacagc gggtggaggc
       61 ggttggacgg agcggtcggc gtgtcgcttc aattagctgc taggggtgaa ggaggagcgt
      121 gaccgaggaa cgggaccgag gggggcaacc ttcaaccttc aaccttcaac cttcaacctt
      181 caactttcaa cttgccatct gccaaccgcc tactggctac tggctactga ctacagccaa
      241 gacccgttcc gcaacatccg acttaagcag gttgtgaccg gtttcgagga tgtggatgtt
      301 ggcctgtgcg tccacctttt tctgatagat tattccgaac gatggccgta tgatgctgtc
      361 gcggcggccg aagaagaggt cggtgcggac ggggtgttgt ttcaaggcgg cgctcagcgc
      421 ggctttgcgg ggcagcaggt agcggaagat catccagacc ttgaacacga gttcgcgctg
      481 cggtccgctg ccgaagtagg agagggcgaa cttgtactgc ttctcgccga ccagcccgag
      541 cgtgcgcaac catccgccaa ggcggaggaa tcgttgcgga cgtctcagca cgcggcggaa
      601 gagccagcgt ccggcgatgg tgcgcgtcac gaagacgtac cagggactga tcaccagtcc
      661 gtcgggcgcg agcaggatca aacgatcgac acgacccgcg aagagcgaaa ccagttcgag
      721 gcagatgcga ccgcccaggc tgaatcctaa cagggaaaat cgagcatgcg gaaagcgctc
      781 gagcagttgg gtgatcagcg cctgcaggtc ttcgcggcgg aattgcagtt ggtcgggcgc
      841 cccttcggcg taactgcggc cgtggtagaa caggttgacg gccacgatgg tgtactgttg
      901 accgagcaag ggctcgaaga gccggaaatc gtctgcgttg ttgtcgaacc cgtgaaatgc
      961 cagcaagagt tcaggtccat tgccaaacac caggtattcc caattaaacg gaccgtgctt
     1021 gactgactgc gggcttgaca cgcgacgaaa gtacgcaaag gagcaccggc gatggtgtgc
     1081 gggcgggcac cgatgcttat caacatgctg tcgaaaactc gggaaaacgg gagccacatt
     1141 tcctgtatct tcgcgcaaac ccgtgggtct ttaccgctgc tcccttttca atttgtaatt
     1201 tattgaaaat taattagtta caaatccggc aaggggcccg ggaggacaca cgcaacctga
     1261 ccatcgtgcc gcgcgatacc agcatccatt ccgtcctcat cattggttcc ggtcccatca
     1321 tcatcggaca agcttgtgaa ttcgattatt cgggcagcca ggcttcccgt tcgctgaaag
     1381 aagaggggat cgaagtgatc ctgatcaact cgaacccggc gacgattatg accgacaagg
     1441 tcacggccga ccacatctac ctgaagccgc tcacccggaa gtcgatcatc gagatcctgg
     1501 aaaagcacaa gatcgacgcg gtgcttccta cgatgggcgg acagaccgcc ttgaatctgg
     1561 cgatcgactg ccagaaggcc ggcatctggg aaaagtacgg cgtgcgcatc atcggtgtcg
     1621 acatcgacgc gattcacacg accgaagacc gggagaaatt ccggctgcgc atgctggagc
     1681 tgaatgccgg cgtctgcaaa ggccgtacgg ccacctcgtt tctcgaaggc aaggagatcg
     1741 cccaggagat cggctttccg ctggtgattc gtccgtcgtt tacgctgggt ggcacgggcg
     1801 gtggtttcgt gaacacgcgg gaagaattcg acgctgcgct gaaccgtggc ctgcatgctt
     1861 cgccggtgca cgaggtgctg atcgagcagt cgattttcgg ctggaaggag ttcgaactcg
     1921 agttgctgcg cgacgccgcc ggaaacgtga tcatcatctg ttcgatcgag aacttcgatc
     1981 cgatgggtat ccatacgggc gattcgatca ccgtggcccc ggcgctcacg ttgaccgaca
     2041 aggaatatca gatcatgcgt gatgcctcga ttgcggtgtt gcgcgaaatc ggcgtggata
     2101 cgggcggttc caacgtgcag ttcgctgtaa accccgccaa tggccgcat
//
LOCUS       DAOTON010000512         2146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_39986_length_2146_cov_2.750359, whole genome shotgun sequence.
ACCESSION   DAOTON010000512 DAOTON010000000
VERSION     DAOTON010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39986_length_2146_cov_2.750359"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="P5292_09800"
     CDS             <1..634
                     /locus_tag="P5292_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39460.1"
                     /translation="GSSLAINGNESRASTPNNYWIPGDWYFVVATYDGSRHKFYVNNQ
                     LIVDQAASGPLSTYGQEMYFGNNTTLDDFFQGVIDDIRIYDRALDSSEVAALYYEELC
                     FDHVTVTDTLIINANLTSFRPVTYQNSIRVYPNPGRDHITIDCGSNYGTMNGYSLRID
                     NTMGQTVFTSTITMQSYYLDLNTWTGNGVYFIYLINAQGNVVDVRSIVIQ"
     gene            complement(703..1575)
                     /locus_tag="P5292_09805"
     CDS             complement(703..1575)
                     /locus_tag="P5292_09805"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HRS39461.1"
                     /translation="MMIPKGKLISIGGNEDKGTEPEPLFTQKNNLNFFELQILSRIMH
                     ECGGHDAHIEVITSASSIPEEVGQNYLDAFGKLHCTNVRIMDIRNREDAQNPEMIERI
                     RNCDGVMFSGGNQLRLSSIFGGTQFLEIITERYFHEDGFVIAGTSAGAMAMSNTMIYQ
                     GSSSEALLKGEVKITTGLAFMRDVIIDSHFVKRGRFGRLTQAVAANPSCIGIGLGEDT
                     GVLVTEGRYMEAIGSGLVIIIDGHHIRHTNIADLKEGSPISIENIIVHVLAKGNCFDL
                     KERKFYVEGVTQNK"
     gene            1766..>2146
                     /locus_tag="P5292_09810"
     CDS             1766..>2146
                     /locus_tag="P5292_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRS39462.1"
                     /translation="MKIQDIRVMRGPNYWSIRRHKLIVMKLDIEELEDLPTNKISGFS
                     ERMEKMFPSMYSHECSEGHAGGFFKRVREGTWMGHVVEHIALEIQTLAGMDVGFGRTR
                     STGQHGVYNVVFNYMEEKVGIYAAK"
BASE COUNT          507 a          503 c          572 g          564 t
ORIGIN      
        1 aggctcgtca ctggcaatta atggaaatga aagtagagca agtacgccga ataactattg
       61 gataccgggc gactggtatt ttgttgtagc tacgtatgat ggttcaaggc ataagttcta
      121 tgtcaataac cagttgatcg tcgatcaagc ggcttccggg ccacttagta catacggcca
      181 agagatgtat tttggaaata atactacatt ggatgacttc ttccagggag ttattgatga
      241 tatccgaatt tacgaccgtg ccctggattc ttctgaagtg gcggcgcttt attatgaaga
      301 actctgtttc gatcacgtaa cggtaaccga tacactcatc atcaatgcga acctgacctc
      361 cttccgtccg gtaacgtatc agaattcaat ccgggtttac ccgaatccag gccgtgatca
      421 tatcacgatt gattgtggat caaattatgg tacgatgaat ggctattcac ttcgaatcga
      481 taatacgatg ggtcagacgg tcttcaccag cacgattacc atgcaatcct attaccttga
      541 cttgaacacg tggacaggta atggtgttta cttcatctat ctgatcaatg cacaaggcaa
      601 cgtggtcgat gtgcgaagca ttgtcataca ataatggctt tttggcataa aaaaatcccg
      661 ctgcatcaac agcgggattt ttttatgcta ttaataagcg gtttacttat tctgcgtcac
      721 accctccaca taaaatttcc gctccttcag atcgaagcag ttgcctttgg caaggacgtg
      781 aacgatgatg ttctcaatgg agatggggga gccttccttc aggtcggcaa tgttggtgtg
      841 acggatgtgg tgtccgtcga tgatgatgac caggcccgat ccgatcgctt ccatgtagcg
      901 gccttcggtg accagtacgc cggtgtcctc gcccagcccg atcccgatgc aggatgggtt
      961 ggctgccaca gcctgagtca ggcgtccgaa tcgaccgcgc ttcacgaagt gtgagtcgat
     1021 gatcacgtca cgcatgaaag ctagtccggt ggtgatcttc acttcacctt tcaggagggc
     1081 ttcggagctg ctgccctggt agatcatcgt attcgacatg gccatggcgc ctgcactcgt
     1141 gccggcgatg acgaaaccgt cctcgtggaa gtagcgctcc gtgatgatct ccaggaactg
     1201 ggttccgccg aagatggagg agaggcgtag ctgatttccg ccgctgaaca tgactccgtc
     1261 gcagttgcgg atgcgctcga tcatctccgg gttctgagcg tcttcccgat tgcggatgtc
     1321 catgatacgg acattcgtgc aatgcaactt gccgaaggcg tcgaggtagt tttgtccgac
     1381 ttcttccggg atggaggatg cgctggtgat cacctcgatg tgggcgtcgt gtccgccgca
     1441 ctcgtgcatg atcctggaca ggatctgcag ctcgaagaaa ttcaggttgt tcttttgggt
     1501 gaaaagtggt tcgggttctg tccccttgtc ttcgttcccg ccgatggaga tcagtttacc
     1561 cttcggaatc atcatattcg tttgactgaa agcgttttgg ctgcgcaaag atagaaatcc
     1621 ccgcatagga cgggcgtttc agcactatat aacgtccgga aatcgggaag gatacgggtt
     1681 cgatggcctt tttcgggcaa attttggcgg ttcaggattt cttactactt tgaaacgacc
     1741 aaaaacactg ataccccctt tccttatgaa gatacaggac attcgcgtga tgcgcgggcc
     1801 aaattactgg tccattcgac gtcacaaatt gattgtgatg aagctggaca ttgaagagtt
     1861 ggaggatttg ccgacgaaca agatcagcgg cttctcggaa cgtatggaga agatgtttcc
     1921 ttccatgtat tctcacgaat gttcggaagg acatgcaggt ggattcttca aacgcgtgcg
     1981 cgaaggaacg tggatgggtc acgtggtgga gcatatcgca ctcgagatcc aaacgcttgc
     2041 gggaatggat gtcggcttcg gccgcacacg cagtaccggt cagcatggtg tatacaatgt
     2101 cgtgttcaac tacatggaag aaaaagtcgg gatctatgcc gctaag
//
LOCUS       DAOTON010000513         2142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40070_length_2142_cov_3.589363, whole genome shotgun sequence.
ACCESSION   DAOTON010000513 DAOTON010000000
VERSION     DAOTON010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40070_length_2142_cov_3.589363"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="P5292_09815"
     CDS             complement(<1..1466)
                     /locus_tag="P5292_09815"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRS39463.1"
                     /translation="MIRSIFLIGLALLASLRSFAAGEVERLALVAKSWGIVKYYHPAL
                     ASGRINADSLLLTMLQQVDDAGAEKTAEDVLLSIITRLGPVSSGACDSKRLLEGKEAV
                     ASFTWIDGLMNNALRGRLKELSNVRCDGKHVSLNATPNASQITSVSSKERGDTLPIPD
                     LTHRYLSLFRAWNAIKYFSPLAAGAGWDAALDELIPAFRNATDQDSYQLACLRMLALL
                     GDGQAKAHSKFLQQYWGSFQVPFDATQAEGDVLVTRGTNDSLLSACGLKIGDRVLKMG
                     NADINARIANRRQFIGASRQSVQDERILRLLFLDRKQSALVLTLNRKGVELIDTVQRL
                     PLHDLGRMREQPPAVEALSGGYLLRAHRINTATQLRQLMDSLRAAPKLVIDLRDNVAP
                     GMESNWLQQLGGVQTFLLPQRVPFMTRPGYYFQRTDTLRPNAKFPVPPFTGKLFLLVN
                     EETQGAAERLAFLLQQLPGTTTVGSPSAGACGSTSFLSL"
     gene            complement(1482..>2142)
                     /locus_tag="P5292_09820"
     CDS             complement(1482..>2142)
                     /locus_tag="P5292_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HRS39464.1"
                     /translation="WKVSLRDLPFVVSEKLNGATTVAATMHIAQLAGIRVFVTGGIGG
                     VHRGAETSMDISADLTEMGRTGVAVISAGVKSILDIGLTLEYLETLGVPVVTFGSEEF
                     PSFYSRGSGFQSPLRLDSPDAIARFLRAKWDLELNGSALIANPIPSEQEFPFAEMEQH
                     IRAALDAAERQSIRGKSTTPFLLKYIADATGGDSLASNIALVKNNAALGARIATAFSS
                     L"
BASE COUNT          472 a          617 c          609 g          444 t
ORIGIN      
        1 agcgagagga aactcgtcga tccgcaggct ccggccgatg ggcttccgac cgtcgttgtt
       61 ccgggaagtt gttgcaataa aaacgcaagc cgctcggccg caccctgtgt ttcttcgttc
      121 accaacagga acagttttcc ggtaaacgga ggcactggaa actttgcatt gggacgcaag
      181 gtatccgtac gctgaaaata gtatccgggt cgggtcatga aaggaacacg ctgcgggagc
      241 aggaacgttt gtacaccgcc caactgctgc agccaattgg attccattcc gggcgcaacg
      301 ttatcacgca ggtcgatcac cagcttaggc gctgcccgca acgaatccat gagctgacgc
      361 aactgcgtgg cggtgttgat tcggtgcgca cgcaacaggt agccgcccga cagggcttcc
      421 accgccggcg gttgttcccg catacgtccc aggtcgtgca ggggcaatcg ttgtacggta
      481 tcgatcagct caacgccttt tctgttcagc gtcaacacga gcgccgactg tttccggtcc
      541 aggaacagca accgaagaat gcgctcgtcc tggacagact gcctcgaagc accaatgaac
      601 tggcggcggt tcgcgatgcg cgcgttgata tcggcattac ccatcttgag cacacggtcg
      661 ccgattttca gaccacaagc cgacaggagt gaatcattcg tcccgcgggt aaccaataca
      721 tcgccttccg cctgagtggc atcaaatggc acctgaaagg aaccccaata ctgttggaga
      781 aacttgctgt gtgcttttgc ctgtccgtcg cccaacaacg ccagcatccg caggcaggcc
      841 aattgatacg agtcctgatc cgtcgcgttc cggaatgccg ggatcaactc atccagggcc
      901 gcgtcccagc cggctcccgc agccagcgga gaaaaatatt tgatcgcatt ccaggcgcga
      961 aagagcgaca agtaccgatg tgtcagatcc ggaattggca gggtgtcgcc tctttccttt
     1021 gacgaaacgc tggttatctg cgatgcattg ggcgtcgcgt tcagcgaaac atgtttcccg
     1081 tcgcaacgga cattcgataa ttccttcaag cgtccgcgaa gggcgttgtt catcagtccg
     1141 tcgatccagg tgaaggaagc aaccgcttct tttccttcca acaggcgttt gctgtcgcag
     1201 gcaccgctgc tgacagggcc aagtcgggtg atgatcgata acagaacatc ttccgccgtc
     1261 ttttccgctc ccgcgtcatc gacctgttgc agcatggtca acagcaaact gtccgcgttg
     1321 atccggccgg aagcgagcgc ggggtgataa tacttgacga tgccccagct tttggcaacg
     1381 agtgccaaac gttctacttc accggcggca aagctgcgaa gggaagccag cagagccagt
     1441 ccgatcagga agatggatct tatcatgtca ggaaagttcg gtcacaaaga tgaaaatgcc
     1501 gtggcgatac gggcacctaa ggcggcatta ttcttcacca atgcgatgtt ggaagcgagg
     1561 ctgtcgcctc cggtggcatc ggcaatgtac ttgagcagga agggcgtggt cgatttaccg
     1621 cgaatgctct gccgttcggc cgcatcgagc gcggcgcgaa tgtgctgttc catttccgca
     1681 aagggaaact cctgttcaga cggaatcggg ttcgcgatca gggccgagcc gttcaattcg
     1741 aggtcccatt tcgcacgcag gaaacgcgcg atggcgtccg gtgaatccag ccgaagcggt
     1801 gactggaagc cgcttccgcg ggagtaaaag ctcggaaact cttcacttcc gaatgtgacc
     1861 accgggactc cgagtgtctc gaggtattcg agtgtaagtc cgatgtcgag gatcgacttc
     1921 actcccgccg aaatgactgc aacaccggtt cggcccattt cggtgaggtc agcgctgatg
     1981 tccatgctcg tttccgcacc acggtgaaca ccgccgatcc caccagttac gaaaacgcgg
     2041 atgccggcca gttgagcgat gtgcatggtc gcggcaacgg tggtcgcgcc attcaacttt
     2101 tccgatacca cgaagggcag gtcgcgcaga ctcactttcc aa
//
LOCUS       DAOTON010000514         2142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40071_length_2142_cov_3.455199, whole genome shotgun sequence.
ACCESSION   DAOTON010000514 DAOTON010000000
VERSION     DAOTON010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40071_length_2142_cov_3.455199"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="P5292_09825"
     CDS             complement(<1..1246)
                     /locus_tag="P5292_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS39465.1"
                     /translation="MLAFETRFMIPSRRLHFLFIFLLTSISLFAQKEPKYTSTDSRAI
                     RAFETAGGYYDSRQDEQALKFLQGAIDADPNFVEAYMMRANILTDQRLYPQAIAAYKK
                     AIEINPDFFPNNYYSLARVEFSEGLYSDAHTHLDKFLTYPKINAGLAAKARQLSTSCA
                     FAEQAVKSPVPFKPVNMGSAINTPEEEYFPAITADGATFLFTRRSRFRDPAGRANKQE
                     DFYVSHKNADGNWSDAAPVAEINTSGNEGAPALSADGQYLFFTACEEMLSNDARKTKG
                     SCDLFLTKKVGDRFGPVRNLEMPINTGAWESQPSFSSDGRTLYFVRAITGSDGRRQRD
                     ILMTRIGDNSAWTEPVSVSDKINTAGDEMSVFIHPDDQTLYFTSDGHPGMGGLDIFLA
                     RRQPDGSWSEPVNLGYPINTGAD"
     gene            complement(1309..1932)
                     /locus_tag="P5292_09830"
     CDS             complement(1309..1932)
                     /locus_tag="P5292_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRS39466.1"
                     /translation="MQPSDPANGSRLPVMEHYFTIQGEGANTGQAAYFIRLGGCDVGC
                     VWCDVKESWDASVHPVYTLETIVGWVKDAGARRAVITGGEPCMYELDGLTARLRQEGI
                     ATYLETSGAYTISGTWDWICVSPKKFKPVLPASLAKANELKVVVFNRHDLSWAQEQAS
                     GTSAGTRLFLQPEYSVRDKVLPEIIAFVKRHPEWTISLQTHKFMQIP"
     gene            complement(1953..>2142)
                     /locus_tag="P5292_09835"
     CDS             complement(1953..>2142)
                     /locus_tag="P5292_09835"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658144.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRS39467.1"
                     /translation="VGITRVPAPETKSGFRIVGDVDFESVAPKCSYISPVPGGVGLMT
                     IIGLLKNTLRAARKEVRF"
BASE COUNT          485 a          574 c          583 g          500 t
ORIGIN      
        1 catcggcgcc ggtattgatc ggatatccca ggttaaccgg ttcgctccag cttccatcgg
       61 gctggcggcg agcgaggaaa atatcgagac cacccatgcc cggatgcccg tcggaagtga
      121 aatagagcgt ctgatcgtcg gggtgaatga aaaccgacat ttcatcacca gcagtattga
      181 tcttatcgct gacagaaacc ggttcggtcc aggctgaatt gtctccaatg cgcgtcatca
      241 aaatatcgcg ctggcgacga ccatcgcttc cggtgatcgc gcgaacgaag tacagggtac
      301 gaccatccga tgaaaaagac ggctgacttt cccatgcgcc cgtattgatg ggcatctcca
      361 gattacgcac cggcccgaat cgatccccga ctttcttggt caggaaaagg tcgcagcttc
      421 ctttggtctt tcgagcgtca ttggagagca tctcttcgca agcagtgaag aacaagtact
      481 gcccgtccgc agaaagcgcc ggagcgcctt cattgccact ggtgttgatc tctgctaccg
      541 gagccgcatc cgaccagttt ccgtcggcat ttttgtgact tacatagaag tcctcttgtt
      601 tgttggctct gccggcagga tcccgaaagc gcgagcgccg cgtgaacagg aaggttgctc
      661 cgtcggcggt aatcgccggg aagtactctt cttccggagt gttgatggcg gaacccatat
      721 tcaccggctt gaacgggacg ggcgacttca cagcttgctc ggcaaaggca caactcgtcg
      781 acaattgtct ggcctttgct gccaggccgg cattgatttt cgggtaggtc agaaacttgt
      841 cgaggtgtgt gtgggcatcg ctgtagaggc cttcactgaa ttcgactctg gcaagagagt
      901 aataattgtt cggaaagaaa tcaggattga tttcaatggc cttcttgtat gccgctatgg
      961 cttgcggata caatcgttga tcggtgagga tattcgcccg catcatgtag gcttccacaa
     1021 aattcgggtc ggcgtcgatc gcgccttgta aaaacttcag cgcctgctca tcttgacggg
     1081 agtcgtaata gcctccggcc gtttcgaatg cacggatagc gcgggaatcg gttgacgtat
     1141 acttcggctc cttttgagca aagagggaaa tcgaagtgag cagaaaaatg aacaggaaat
     1201 gcagcctgcg ggaagggatc atgaagcggg tttcgaatgc taacacttca aagatattgc
     1261 cacgcccatc ctgtcgattg ctcgaaagaa ccgattttca acaaaccatc agggaatctg
     1321 catgaacttg tgcgtttgca gggaaatcgt ccattccggg tgacgtttga cgaaggcaat
     1381 gatctcggga agtactttat cccgcacact gtactccggc tgtaagaaga gcctcgttcc
     1441 tgcggaggtc ccggaagcct gttcctgtgc ccaggacaag tcgtggcggt tgaaaaccac
     1501 cactttcaat tcattcgctt tggccagcga tgcaggaaga accggtttga acttctttgg
     1561 agagacacag atccaatccc aggtcccgct gatcgtataa gcacccgatg tttcaagata
     1621 tgttgcgatc ccttcctgtc ggagccgagc agtcaggcca tccagctcat acatgcaagg
     1681 ttctccgccg gtgatgaccg cccgtcttgc acccgcgtcc tttacccaac ccacgatggt
     1741 ttccagggtg tataccggat gcacggaagc gtcccaggat tccttgacat cgcaccatac
     1801 gcatccaaca tcacaaccgc cgaggcgaat gaaatacgcg gcctgaccgg tatttgcgcc
     1861 ttcaccctgg atggtgaaat agtgctccat gaccggaagc cgggatccgt tggctggatc
     1921 ggatggttgc atgtaacagg aattttgtgg ggtcagaagc gcacttcttt gcgcgctgcc
     1981 cgcagcgtat tcttcaagag accgatgatg gtcatcaacc cgactccacc cggtaccgga
     2041 ctgatgtaac tgcatttggg tgctacggat tcaaagtcga catctcccac gatgcggaat
     2101 ccgcttttcg tttccggcgc cggtactcgg gtaatgccga cg
//
LOCUS       DAOTON010000515         2135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40233_length_2135_cov_3.836538, whole genome shotgun sequence.
ACCESSION   DAOTON010000515 DAOTON010000000
VERSION     DAOTON010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40233_length_2135_cov_3.836538"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1152)
                     /locus_tag="P5292_09840"
     CDS             complement(16..1152)
                     /locus_tag="P5292_09840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS39468.1"
                     /translation="MAIRKILIVTNIPTPYRIPLFNEVYRQLKVRGIELKVLFGAEGY
                     ARRKFRFQAEQCQFPFAFLGSSSVAFGDSEKTMFSYGGIMKAIRAEKADRVVVSGFSL
                     ATMRLWWRSWWARQEYIIWSGAIAHPGRNDSFLRRLQRRLVVSRASGFLAYGTLAKDY
                     LIGLGARPDRVGLAFNTVDTTFFSEETEGLRKSLTAGPPFHLTYVGYLSARKNVVRVL
                     EVVKKLKESRTDFVLDLVGDGDQREELENWAKNNGLETCVVFHGYKQKEDLPAILARS
                     SVFLFQTDFDIWGLVLNEAMAAGLPVICSPHAGAAPDLVVEGETGFIMDYADTIGIAN
                     RVSQLLDYPEQARQMGIRAADRIRQRFSLTDSARAYVETLLAEG"
     gene            complement(1159..>2135)
                     /locus_tag="P5292_09845"
     CDS             complement(1159..>2135)
                     /locus_tag="P5292_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HRS39469.1"
                     /translation="KKFGTDHYERQVTPDDIREFLPKMVDIFDEPLADATCIPIHFIS
                     EKARENGTIVVLTGDGSDELFAGYRNWMRVAGLYPAFERFRAMPAMLKRPVAGLYGLL
                     DSTSPRYEILSRAARDQEFFWGGAKSFKESTKRDFLSEDYNRRTAELDSHSVIASFRR
                     QYEEADRRSDSSPLDWMCYLGFKFNVPNYYLHRMDRLGMAHSIEVRTPFLDYQFVNMA
                     LNIPEKWKIRDGEPKWILKKSLEGLLDHDILYRKKRGFNVPLKAWAGDLMVDYVSKNL
                     RPFCNDFPQFRYEGLHRQVERLKGGNEQVANTLWTLYYLMAWFRRWLG"
BASE COUNT          507 a          614 c          546 g          468 t
ORIGIN      
        1 aacatcagag ccgcattaac cttcggcaag cagggtttcg acatacgcac gcgcgctgtc
       61 ggtgagagag aatcgctgcc ggatgcggtc cgctgcacgg atacccatct gccgcgcctg
      121 ttcgggataa tcgagcaatt gactcacccg atttgcgatg ccgatggtat cagcatagtc
      181 catgatgaaa cccgtttcac cttccacgac gagatccgga gcagccccgg catgcggcga
      241 gcaaatgacc ggcaatcctg cggccattgc ttcgttcaac accagtcccc agatatcgaa
      301 gtcggtctga aacaggaata cactactgcg agccagaatg gccggcaaat cttccttctg
      361 tttatacccg tgaaatacca cacaggtttc caaaccgttg ttttttgccc aattttcaag
      421 ctcttcacgt tgatcgccat ccccgaccag gtcaagaacg aaatcagtac gcgattcttt
      481 gagctttttg accacttcca ggaccctgac cacattcttc ctggcggaga gataacctac
      541 ataggtcaga tgaaaaggcg ggccggcggt aagggatttt cgaaggcctt cggtttcttc
      601 ggaaaagaag gtcgtatcga ccgtattaaa ggctaagcca acccgatccg gccgtgcacc
      661 cagcccgatg agatagtctt tggccagtgt tccataagcc aggaaaccgc ttgctctgct
      721 gacgaccaat cgtctctgca ggcgacgtaa aaacgagtcg tttcgacctg gatgcgcgat
      781 ggcaccggac cagatgatat actcctgtcg ggcccaccag gatctccacc aaagccgcat
      841 ggttgcaagt gaaaagccgc tgaccactac ccgatccgcc ttctcagcgc gaatcgcttt
      901 catgatgccc ccataagaga acatggtctt ttcggaatcg ccaaacgcca ccgaggagga
      961 accgaggaaa gcaaagggga actgacattg ttccgcctgg aacctgaact tccgacgcgc
     1021 atatccctca gcgccaaaga gcactttcaa ctcgatgcca cggactttca actgccggta
     1081 cacctcattg aaaagcggga tccggtaggg agtcggaata ttggtaacga tgaggatctt
     1141 ccggatggcc atgatcggtt aacccagcca ccggcggaac caggccatca ggtaatagag
     1201 ggtccaaagg gtgttggcca cctgttcgtt cccccctttc aggcgttcca cctgccggtg
     1261 tagtccttca tacctgaact gagggaaatc gttgcagaaa ggccggagat ttttgctgac
     1321 ataatcgacc atcaggtcac ccgcccaggc ctttaggggt acgttgaacc cccgcttttt
     1381 ccggtacaaa atatcgtgat ctagcagtcc ctcgagcgac tttttcagga tccatttcgg
     1441 ttctccatcg cggatcttcc acttttccgg gatgttcagc gccatgttga caaactgata
     1501 gtccagaaaa ggcgttctca cctcgatgga gtgtgccatg cccagccggt ccatgcgatg
     1561 gaggtaatag ttgggcacgt tgaacttgaa tccgaggtag cacatccagt ccagcggact
     1621 gctgtcactt ctccggtcag cttcctcgta ctgccgacgg aacgaagcga tgaccgaatg
     1681 actgtccagc tcagctgtcc gacgattata gtcttcggat agaaaatcac gcttggtact
     1741 ctccttaaag ctcttggctc ctccccagaa gaattcctga tcacgcgccg ccctggataa
     1801 gatctcgtag cggggagagg tgctatccaa caagccgtac aaaccggcaa ccggacgttt
     1861 aagcatggca ggcattgccc gaaaacgttc gaaggccgga tacaacccgg ccacccgcat
     1921 ccagttgcga taaccggcga agagctcatc actgccgtcg ccggtcagta cgacaatggt
     1981 cccgttctcc cgggcttttt ccgaaatgaa atggatcgga atgcaggtcg cgtcagcgag
     2041 cggctcatca aagatgtcca ccatcttcgg gagaaactcc cggatatcgt cgggggtgac
     2101 ttgccgctcg tagtggtcgg taccgaattt tttgg
//
LOCUS       DAOTON010000516         2135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40235_length_2135_cov_3.230769, whole genome shotgun sequence.
ACCESSION   DAOTON010000516 DAOTON010000000
VERSION     DAOTON010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40235_length_2135_cov_3.230769"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..541
                     /locus_tag="P5292_09850"
     CDS             131..541
                     /locus_tag="P5292_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39470.1"
                     /translation="MKRILILALFLITAGLSGCEKVEMLKSEKSVEKELEGTWNLIPI
                     PRTNPAEEWAFRENTIIRSQMLGSVMTPIDTGHYTIRTTLMKSYVKFEDFSLVLDELN
                     GEWQIVELSSNVFVIATDHNGATGIMELDFSRKD"
     gene            661..2037
                     /gene="radA"
                     /locus_tag="P5292_09855"
     CDS             661..2037
                     /gene="radA"
                     /locus_tag="P5292_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRS39471.1"
                     /translation="MAKAKTVFYCQNCGAQSPKWVGRCASCGQWNTYVEEVVQKETSA
                     NLWKKPEDRKRKTPKPQLLQDISAGGEKRVVLPDAELNRVLGGGLVPGSLVLLGGEPG
                     IGKSTLVLQVALGIKGVPVLYVSGEESEQQIKMRADRLKLRNDRFYVLTETQLENIFQ
                     QVAETDPGLLVIDSVQTIFTDKIESSPGSVSQIRECTAELLRFSKQSGVPVFLIGHIT
                     KDGTIAGPKILEHMVDTVLQFEGDRHHLYRILRSVKNRFGSTAELGIYEMQSSGLREV
                     SNPSEVLIAQREELLSGVAIAATMEGVRPLLIETQALVSSAVYGTPQRSSTGFDLRRL
                     SMLLAVLEKRCGFRLGAKDVFLNLAGGIRVEDPAIDLAVICAILSSNEDIAITPKICF
                     AAEVGLSGEIRPVARVEQRIGEAEKLGFEQIIISKYNKKGLDLSRFKGIRIHAVSKVE
                     EVFSLLFG"
BASE COUNT          518 a          572 c          588 g          457 t
ORIGIN      
        1 accatcgagt tcctacccgg atattcaaca agtgcgatag aaaagcggat tttggacgga
       61 aaaagccgtc cgtaagaaag gtgaaggatt ttgaacaact ttattccgta tctttcataa
      121 aaccagtatc atgaaaagga tcctcatcct cgcgctgttt cttattactg ccggcttgtc
      181 tggttgtgag aaagttgaaa tgctcaaatc tgaaaagagc gttgagaaag aactggaagg
      241 tacctggaat ctgattccca ttccgcgaac gaatccggct gaagaatggg cattccggga
      301 gaataccatc attcgaagcc agatgcttgg cagcgtcatg acaccgatcg ataccggtca
      361 ctatacgatc aggacaaccc tgatgaaatc ctatgtcaag tttgaagact ttagtctggt
      421 gctggatgaa ttgaatggcg aatggcagat cgttgaacta agcagcaacg tcttcgtgat
      481 cgctactgac cataacggag caaccggtat catggaactc gacttttcaa gaaaagactg
      541 attcctctat ccccctcatt tgaaaagcga aggcatcccc ttcgcttttc tcattttatg
      601 gaacggttat agccagccga ctctgaacgc aatgtagaat tggcgtaact tcggcgcgcc
      661 gtggccaaag cgaaaaccgt cttctattgc cagaattgtg gcgcccagtc acccaagtgg
      721 gtcggacgtt gcgcatcctg cgggcaatgg aacacctatg tggaagaggt ggttcagaag
      781 gaaacatccg ccaatctctg gaagaagccc gaagaccgta aacgcaagac gccgaagccc
      841 caattgctgc aggacatcag cgccggcggt gagaaacgcg tagtcttgcc ggatgcggaa
      901 cttaatcgcg tgctgggcgg cggactggta ccgggatccc tggtgttgtt gggtggcgaa
      961 ccggggatcg gtaaatccac cctggtgctg caggtcgccc tgggtattaa aggagtaccg
     1021 gtactgtatg tcagcggaga agaaagcgag cagcagatca agatgcgcgc cgatcggctg
     1081 aagttgcgca acgaccggtt ctatgtgctg accgagaccc aactcgaaaa catctttcag
     1141 caggttgccg agacggatcc gggcttgctg gtcattgatt ccgttcaaac gattttcacc
     1201 gataagatcg aatcctcgcc cggaagcgtt tcgcagatcc gcgaatgcac ggctgaactg
     1261 cttcgctttt ccaaacagtc gggcgtaccg gtattcctga tcggacacat caccaaagac
     1321 ggcaccatcg ccggaccgaa gatcctcgaa cacatggtcg atacggtcct gcaattcgaa
     1381 ggcgaccggc accacctcta ccgcatcctc cgttcggtca agaaccgatt cggatcgacc
     1441 gccgagttgg gtatttacga aatgcaaagc agcggcttgc gcgaggtttc caatccttcg
     1501 gaggtgctca tcgcacaacg cgaagagtta ctgagcggag tcgcgatcgc cgcgacgatg
     1561 gagggcgttc gccccttgct catcgagacg caggcactgg tcagctcggc ggtgtacggc
     1621 acgccccagc gctcttcgac gggattcgat cttcgtcgtc tcagcatgtt gctggccgta
     1681 ctcgagaaac gttgcggctt ccggctgggc gcgaaggatg tattcctgaa cctggccggt
     1741 ggcatccgcg tggaggatcc ggcgatcgac ctggccgtca tctgcgcgat cctttcttcc
     1801 aacgaagaca tcgcgatcac gccgaagatc tgttttgccg cggaagtcgg actgagcgga
     1861 gagatccggc cggtggctcg cgtcgaacaa cggatcggcg aagccgagaa gctcggcttc
     1921 gaacagatca tcatcagcaa gtacaacaag aaggggctcg acctctcccg cttcaaaggc
     1981 atccgcattc atgccgtatc gaaagtggaa gaggtctttt cgctgctctt cggttgaatc
     2041 aacggtgcgc gatcaccgcc ggcagattcc acacctttcc ttcgaactcg acccgcaatc
     2101 ggtacaggcc ggaaggccat gaaccggtat cgatc
//
LOCUS       DAOTON010000517         2134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40270_length_2134_cov_2.307840, whole genome shotgun sequence.
ACCESSION   DAOTON010000517 DAOTON010000000
VERSION     DAOTON010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40270_length_2134_cov_2.307840"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..1044
                     /locus_tag="P5292_09860"
     CDS             127..1044
                     /locus_tag="P5292_09860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS39472.1"
                     /translation="MAEAQRQLLSIVIVSYNVRELLEACLRSLPMGGVEEPEVWVVDN
                     ASTDGSVEMVRHLFPTVNLIVNQDNAGFPKGNNQAIRRCSGKFILLLNPDTVVKQDAF
                     KILISYLEDHPEAGLVGPKLLNADGSHQYSVMPFLRPLEIILETFFLHGWYRSKRDHR
                     MQGDQAPRPVDALSGAAILVRREVFDRIGLLDEQLFWTEDMEFCLRAHQARIGVAYMP
                     EAEIVHHSGASGKKNQRVMVSNQVLSKIRYFSRNHPALVFWTCWFFRLLHIISRLLIL
                     LPLSIFALRYREKLDAYAFTLKRFIRGDY"
     gene            1047..1865
                     /locus_tag="P5292_09865"
     CDS             1047..1865
                     /locus_tag="P5292_09865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS39473.1"
                     /translation="MAPDALILLVNYRHEDEVVTFIREQLLQQEQVELTIVVCDNGST
                     DSDGFRKQLSAWKQVHYFSAGTNLGYFGGAAAGLKYFTQTIGELPSWTIVCNTDVELP
                     GRDFLYRLFSLSRGALVAAPAVHSTLSGHQQNPFREQRVSLSYLKFMRAIYSFYPLYF
                     LYQLLAYVKRFFRRARTAEGVTRNVYAVHGTFFAFHRTFFAQGGSLQYGSFLFGEELF
                     VAEQLAGMQSACRYVPELQIEHREHASTGRFKSPGMVKYMKESTDYLIREVYRG"
     gene            1858..>2134
                     /locus_tag="P5292_09870"
     CDS             1858..>2134
                     /locus_tag="P5292_09870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS39474.1"
                     /translation="MDKTPFVSIIIPCRNEGRFIGKVLENILSQDYPASHLEVLIADG
                     RSEDDTRQQVEGFARRYPFIHLLDNPARVVPHALNACIRRSRGEVILR"
BASE COUNT          475 a          519 c          585 g          555 t
ORIGIN      
        1 ggcaagaagg accggtcagg ccatgctttc tccccgaagc cggcttgtct tatgggcagg
       61 cgtaccgttc atttatccgg tagtgaagtt gggtcgttat ttgcgtaacc tgtcaaagtc
      121 ttgaaaatgg ccgaggcgca acgacagctc ttaagtatcg ttatagtcag ctacaatgta
      181 cgcgagcttt tggaagcctg cctccgcagc cttcccatgg gtggtgtgga agagcctgaa
      241 gtatgggtgg tggacaatgc atccaccgac ggaagtgttg agatggttcg tcatttgttt
      301 ccgacggtga acctgatcgt gaatcaggat aatgcaggat tcccgaaagg gaacaaccag
      361 gcgatccgtc gatgttccgg taagtttatt ttgctgctga atcccgacac agtagtgaag
      421 caggacgctt tcaagatcct gatcagctac ctcgaggacc atccggaggc cggactcgtt
      481 ggaccgaagt tgctgaatgc ggatggaagt catcagtatt cggtgatgcc tttcctgcgt
      541 ccgctggaga ttatcctgga aactttcttt ttacatggtt ggtaccgaag taagagggat
      601 cacaggatgc agggtgatca agcgccacga ccggtggatg ccttgagcgg cgcggctatt
      661 ttggtacgcc gggaggtctt cgaccgaatc ggactgctgg atgaacaact cttctggacc
      721 gaggacatgg aattctgttt acgtgctcac caggcgagaa taggtgtcgc ctatatgccc
      781 gaggcggaaa ttgttcacca cagtggagcc agtggtaaga aaaaccaacg ggtcatggtc
      841 tccaaccagg tgttatccaa aatccgctat ttctcccgca atcacccggc attggtcttt
      901 tggacctgtt ggttctttcg gctcctgcac ataatcagtc ggttgctcat cttactgcca
      961 ttgtccatct ttgctttgcg ctaccgggaa aagctggatg cctatgcgtt tacgttgaag
     1021 cgttttatcc ggggcgatta ttgaccgtgg cacccgatgc gttgatccta ctggtgaatt
     1081 atcgtcatga agacgaggta gtgaccttta tccgggaaca gttacttcag caagaacagg
     1141 tcgagttgac catcgtagta tgtgataacg gaagtacgga tagcgatggt ttcaggaagc
     1201 aattatcggc ttggaagcag gttcattatt tttcagccgg aaccaacctg ggatattttg
     1261 gcggtgctgc agctggtttg aaatacttta cgcaaacgat aggcgagctt ccttcctgga
     1321 ccatcgtttg caataccgac gtggaacttc ccggacgtga tttcttgtac cggttatttt
     1381 cgctatcccg gggcgcattg gtggcggctc ccgcggttca ttcgaccttg agcgggcatc
     1441 aacaaaatcc tttccgggag caacgggtat ccttatccta cctgaagttt atgcgtgcca
     1501 tttattcttt ctatccactg tattttcttt accagctatt ggcctatgtg aagcgattct
     1561 ttcggagagc gcgtactgca gagggcgtga cgcgtaatgt ctatgcagtg catgggacct
     1621 ttttcgcttt tcatcgaaca tttttcgctc agggcggctc cctgcagtac ggatcatttc
     1681 tattcggcga ggaacttttt gtcgccgaac agcttgccgg catgcagtcg gcctgccggt
     1741 atgttccgga attgcagatc gaacaccggg aacacgcttc caccggtcgt ttcaagtcac
     1801 cgggaatggt gaagtacatg aaggaaagca ccgactacct gatccgggag gtataccgtg
     1861 gataagacac cctttgtcag catcatcatt ccgtgcagga acgaaggccg cttcatcgga
     1921 aaagtgctgg agaatatcct ttcccaggat tatccggctt cgcacctgga agtactcatc
     1981 gccgacggca ggagtgaaga cgatacccga cagcaggtgg aaggatttgc acgacgctat
     2041 cccttcattc atctgctgga taatcctgca cgcgtagtgc cacacgcgct taatgcctgt
     2101 atacggcgga gcaggggcga agtgatcctg cgca
//
LOCUS       DAOTON010000518         2131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40337_length_2131_cov_3.604528, whole genome shotgun sequence.
ACCESSION   DAOTON010000518 DAOTON010000000
VERSION     DAOTON010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40337_length_2131_cov_3.604528"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="P5292_09875"
     CDS             <1..251
                     /locus_tag="P5292_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HRS39475.1"
                     /translation="YMKKTADKHGIPWQPEILAGGGTDTAPIQRMGKTGSIAGAVSIP
                     TRHIHQVIEMADRNDIDAAIRLLTACIEGLDAHSWEFK"
     gene            345..1412
                     /locus_tag="P5292_09880"
     CDS             345..1412
                     /locus_tag="P5292_09880"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRS39476.1"
                     /translation="MLSPGLQFLESALNVFDTSDEAVLILDLHGIIQYANGCFLDRLG
                     FTRQEVLHSPCSRYHAELHFRFDDLLSKLAVDEIVIRDIVCVDRKKRERPFRFVGCLL
                     RDPQANVRGVLVVLSSRTTLREEQLEHFAKQRLLLSALNARRDELVTIIDVQLQLTVF
                     VSDSIGPMLGWRPEEYLEGGWPLEFANFHPDDMNHIALVYYREIVRRNQDNALDEQPI
                     QWEFRRRHRSGIWRRIRSESYILERDDRGNIKYLISFEKEVNDEKSEASEAVRIVEAL
                     LNAAPAHALSASSFSSLSLSPREKELVGLLKRGLSAKEMALRMGLTLYTINSYKKKLL
                     AKLNARNSAELVRIAIEQQLI"
     gene            1420..>2131
                     /locus_tag="P5292_09885"
     CDS             1420..>2131
                     /locus_tag="P5292_09885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRS39477.1"
                     /translation="MPGTKRFLQPIRSILESAKSGILIVKADGKVFFMNDSLEQQSGI
                     KFNGLRGKGLNQLFPAGRMAELIRLTEGRQLPKRLRMKMNTASGERVVSVFVSRIMEH
                     TKLDGLLLVIQSDTQEKPNGDFQKNRNSILRVMDRRVDEACSITDILTGEDLFMSSSI
                     AQLTGWEADDFHKGGFGFGMSLIHPDDVGGVMSIFEQEIEKRNREPYLHDHLVWHASF
                     RYRTADGKYIRLRAETLIL"
BASE COUNT          517 a          535 c          575 g          504 t
ORIGIN      
        1 attacatgaa aaagaccgct gacaaacatg ggataccctg gcagcccgag atcctggccg
       61 gcggaggtac ggatactgcg cctattcaac gtatgggcaa gaccggttcg atcgccgggg
      121 cggtatccat tcccacccgt catatccacc aggtgatcga gatggccgac cggaacgata
      181 ttgatgccgc catccggctg ttgacggcct gcatcgaagg gctggatgcc cattcctggg
      241 agtttaaata ggggattttc acccggccat tacctccttc tgcgaaaatt tcgttttgaa
      301 gaattcggcg aaagactaac tttccgtgaa tttgcatttt cgccatgctc tcgcccgggc
      361 ttcagttttt agaatcagcg cttaacgtct tcgatacatc agacgaggcg gtgctgatat
      421 tagacctgca cggcatcatc caatatgcca acggctgctt tctggaccgc cttggattca
      481 cgcgccagga ggtcctccat tctccttgtt ccaggtatca tgctgaattg cattttcgct
      541 tcgacgattt gttatcgaag ctcgcagtgg atgaaatcgt catccgggat atagtttgtg
      601 tcgacaggaa aaaacgggag cgaccattcc ggtttgtcgg ttgcctgctc cgggatcctc
      661 aggcgaatgt gcgtggcgta ctggtagtct tgagcagtcg aactacgcta cgggaggagc
      721 agttggaaca cttcgccaag caacgcttat tattgtcagc cttgaatgcc cgacgggacg
      781 aactggtcac gatcattgac gtgcaattac agttgaccgt gtttgtttcc gactccatcg
      841 gaccgatgct gggctggcga ccggaagaat acctggaagg aggctggcca ctggagtttg
      901 cgaattttca tcccgacgac atgaaccaca tcgcgctggt ttattatcgg gaaatcgttc
      961 gacgcaatca ggataacgct ttggacgagc agcctataca gtgggagttt cgccgacgcc
     1021 accgctcggg aatctggcgc aggatccgaa gtgaatcgta tattcttgaa cgtgacgatc
     1081 gtggcaacat caagtacctg atcagtttcg aaaaagaagt gaacgatgaa aagagtgagg
     1141 caagcgaagc ggtgcggatc gtcgaagcgc tgctgaacgc ggcgcccgct cacgcgctat
     1201 ccgcctcatc gttttcaagt ttaagcctct ccccgcggga gaaggaactc gtcggactcc
     1261 tcaagcgcgg cctgtcagct aaagagatgg cgttgcgtat gggacttaca ctctatacga
     1321 tcaattctta caagaaaaaa ttgttggcga aactgaacgc caggaattcc gccgagttgg
     1381 tgcgcatcgc cattgaacag caattgatct gaatcgttta tgcccggaac caagcggttt
     1441 cttcaaccga ttcgcagcat actggaatcg gcgaaaagcg ggattctcat cgtaaaggcg
     1501 gatgggaaag tctttttcat gaacgacagc cttgaacagc aaagtggtat caagtttaac
     1561 ggtcttcgtg ggaaaggcct taaccaactc tttcccgcag gacgaatggc agagcttatc
     1621 cggctgaccg aaggtcgtca acttccgaaa cgacttcgga tgaagatgaa taccgcttcc
     1681 ggtgaacgcg tcgtttccgt ttttgtcagt cgcatcatgg aacatacgaa gctggatggg
     1741 ctcctgttgg tcatacaatc cgatacccag gaaaaaccga acggcgattt ccaaaagaat
     1801 cgcaactcca ttcttcgagt catggaccgg cgtgtcgacg aagcttgttc gatcaccgat
     1861 attctgacgg gtgaggatct gttcatgagc agctctatcg cgcaactgac cggttgggaa
     1921 gccgatgact ttcacaaagg cgggttcggt ttcgggatgt cgctcattca tcccgacgac
     1981 gtaggaggtg taatgagcat cttcgagcag gaaattgaaa aacggaaccg ggaaccttat
     2041 ctgcacgacc acttggtgtg gcatgcctcc ttccgctaca ggacggcgga tgggaaatac
     2101 atacgcttaa gagccgagac acttattttg g
//
LOCUS       DAOTON010000519         2131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40339_length_2131_cov_3.261561, whole genome shotgun sequence.
ACCESSION   DAOTON010000519 DAOTON010000000
VERSION     DAOTON010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40339_length_2131_cov_3.261561"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="P5292_09890"
     CDS             complement(<1..725)
                     /locus_tag="P5292_09890"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRS39478.1"
                     /translation="MAKNLVIVESPAKAKTIEGYLGKDFVVKSSYGHVRDLVKSGMGV
                     NVDKNFDPVYEVSPEKVDVINELRRIVKKVETVWLATDEDREGEAISWHLFETLGLEE
                     DATRRIVFHEITKKAITNAIANPRKIDRNLVDAQQARRILDRLVGFELSPVLWKKVRP
                     QLSAGRVQSVAVRLIVEREREIDKFEITSAFRVIAFFDDKGKTFRAELPKRFATEAEA
                     QQFLEQCIGAQFSVKNLEVKPGKR"
     gene            1364..>2131
                     /locus_tag="P5292_09895"
     CDS             1364..>2131
                     /locus_tag="P5292_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS39479.1"
                     /translation="MEKFGNKEIDESRQGEALVLPEAGAQRSKLLYLESYGCQMNFSD
                     SEIVASILRDCGYSTTRVLEEADLILLNTCAIRDNAEQRVWGRLRDLRHGKNRKPDQI
                     VGVLGCMAERLKTQLLEQEQLVDIVVGPDAYRDLPNLIAAAGSGQKAVNVLLSREETY
                     AEISPVRLGSNGITAFISIMRGCDNMCSFCVVPFTRGRERSRDPESIVQEARQLVEQG
                     YKEVTLLGQNVDSYLWWGGGPKKDLPETTVQDALNHRL"
BASE COUNT          452 a          653 c          549 g          477 t
ORIGIN      
        1 cgcttgccgg gtttcacttc caggtttttc accgagaact gcgcaccgat gcattgctcc
       61 aggaactgtt gcgcttccgc ttccgtagcg aaacgcttcg gcaattccgc ccggaacgtt
      121 ttgcccttat cgtcgaagaa ggcgatgacg cggaatgccg acgtgatttc gaacttatcg
      181 atctcccgct cacgctccac gatcaggcgg acggcgacgg actgtacacg gccggcggaa
      241 agctgcggac gcaccttttt ccagagtacc ggcgacagct cgaaaccgac gagtcggtcg
      301 aggatgcgcc gtgcctgctg cgcgtcgacc aggttgcggt cgatcttgcg cgggttggcg
      361 atcgcgttgg tgatcgcttt cttggtgatt tcgtggaata cgatccggcg ggtggcatcc
      421 tcttccagcc cgagcgtttc gaacaagtgc caggaaatgg cttccccttc gcggtcctcg
      481 tcagtcgcga gccatacggt ctccactttc ttcacgatgc ggcggagttc gttgatcaca
      541 tccacctttt ccggactcac ttcatagacc ggatcgaaat tcttatcgac gttcacaccc
      601 atgccgctct tcacgaggtc gcgcacatgg ccgtaactcg atttcaccac gaaatctttt
      661 ccgaggtatc cttctatggt tttcgccttt gcgggcgact caacgatcac aagatttttc
      721 gccatccggt tttttcagtc ggcgcaaata aaagcattta ttttttgatc gttcaaacga
      781 cccgggacat ccgaatcatt tctccgcatt tttgcaacgt taaaagccgc caccagcaag
      841 gctttcccga cgttcaacat ttttttcgca cgcatcacta ccgcgcctcc acaacaacct
      901 gctcaaaaca cacacccgtg tcgttcacag tacgttacaa ctttcctgtc cgatgtcctt
      961 ccgaacgttc attcattttt tttcctccag aatccaagcg attcttctgc aaagaagtat
     1021 agatgtggga gcgatgcggg gcgacttcgt ttcaccgggc ttcccgtgtt cactcctcac
     1081 atcagcttgc gcatgttgct caacgcagga agcgtgacga gttcacccgg ggctggattg
     1141 ccacggttcc cttcgcctga acccgaccat gcatacctat atataggcag aattcctttc
     1201 ccccgaccct ggaatcgaga tgggaagcaa gcgtacccgg aaccgcattc agcgaccttt
     1261 tcgattatac aaatcccgtt tttacactaa aaatccagtc cgagtcatca ttaacggctg
     1321 acaacttgtc acgtacccgc caaggggcta tctttgtcat ccaatggaga aattcgggaa
     1381 caaggagatc gacgaatcgc ggcaagggga agccctggtg ctgccggaag ccggggcaca
     1441 gcggagcaaa ctgctttacc tggagagcta cggctgtcag atgaactttt ccgacagcga
     1501 gatcgtggca tccatcctcc gcgattgcgg ctactccacc acccgggtgc tggaagaagc
     1561 cgacctgatc ctgctgaaca catgcgccat ccgcgacaat gccgagcagc gcgtgtgggg
     1621 tcggctgcgg gaccttcgcc acggcaagaa ccgcaaaccc gaccagatcg tgggtgtgct
     1681 cggctgcatg gccgaacggc tgaagaccca actgctcgaa caagaacagc tggtcgacat
     1741 cgtcgtaggt cccgacgcct accgcgatct tccgaatctc atcgctgctg ccggctccgg
     1801 ccagaaagcc gtgaacgtgc tgctctcgcg cgaggagacc tacgcggaga tcagccccgt
     1861 acgcctcggc agcaacggca tcaccgcttt catctcgatc atgcggggtt gcgacaacat
     1921 gtgttcgttc tgcgtggtac cctttacccg cggtcgcgaa cgcagccgcg atccggaatc
     1981 catcgtgcag gaagcacgac agctcgtcga acaaggttac aaggaggtta cgctccttgg
     2041 acagaacgtc gattcctacc tctggtgggg aggcggtccg aagaaagacc tgccggaaac
     2101 caccgtgcag gacgctctca accaccgcct g
//
LOCUS       DAOTON010000520         2131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40346_length_2131_cov_2.678709, whole genome shotgun sequence.
ACCESSION   DAOTON010000520 DAOTON010000000
VERSION     DAOTON010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40346_length_2131_cov_2.678709"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /gene="fabZ"
                     /locus_tag="P5292_09900"
     CDS             <1..705
                     /gene="fabZ"
                     /locus_tag="P5292_09900"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRS39480.1"
                     /translation="VFHKQNVKAERGFLDNVKLRYINEPARHKLLDIVGDLALVGAPI
                     KGHILAARPGHVANVAFARQIKEIMKRERFKERPPKYDPNVPPMFDVNGIMKFLPHRA
                     PFLFIDKILELSDRHVVGVKSVTMNEWFFPGHFPGAPVMPGVIQIEAMAQVGGILVLN
                     TVPDPENYLTYFIKIDNTKFRDMVVPGDTLLFHLELISPIRRGICHMRGKAFVGDKVV
                     MESEMMAQIVRRDKKK"
     gene            747..1535
                     /gene="lpxA"
                     /locus_tag="P5292_09905"
     CDS             747..1535
                     /gene="lpxA"
                     /locus_tag="P5292_09905"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRS39481.1"
                     /translation="MQALNWIHPEAKIGKDVKIDPFTVIHEDVEIGEGTWIGSNVTIF
                     PGARIGKHVRIFPGAVISAVPQDLKFAGEKTTAEIGDHTTIREYVTINRGTTAAGKTV
                     VGAHNLLMAYVHIAHDCFVGDHCILANGVTLAGHIEIHDYAIIGGLSAIHQFVQIGSH
                     VMISGGSLVRKDVPPFVKAAHEPLAYVGVNALGLRRRGFSNDQIAQIQEIYRKVFVSG
                     LNITHAIEAVEQDIPASAERKLILDFIRKSSRGLMKGYAAQSDE"
     gene            1535..>2131
                     /locus_tag="P5292_09910"
     CDS             1535..>2131
                     /locus_tag="P5292_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS39482.1"
                     /translation="MSAFRISLEKAGKRYLREWIFRGVELSLSSGDRIAVMGPNGSGK
                     STLLQLIAGYVLPTEGAVRHFQHDQLVDPDLVYPSISMAAPYLELIEEFTLRESIAFH
                     CSFKQLRNGLMPEDVIRIANLSDAADKVMKYFSSGMKQRVRLALAVLSDTDVLLLDEP
                     CSNLDREGIRWYQQLIEEHGAGRILVVCSNNIAEEYSFC"
BASE COUNT          499 a          533 c          604 g          495 t
ORIGIN      
        1 gtctttcaca agcaaaacgt aaaagccgaa cgtggttttc tggataacgt gaaattgcgt
       61 tatatcaacg aaccggcacg acataaactg ttggatattg ttggtgacct cgcgctcgtc
      121 ggagccccga tcaagggaca catcctggct gcacgtccgg ggcacgtcgc gaacgtcgcc
      181 tttgcccgac agatcaagga aatcatgaag cgggagcgtt tcaaggagcg tccgcccaag
      241 tatgacccga atgtcccgcc gatgtttgac gtgaacggca tcatgaaatt cctgcctcac
      301 cgtgcgccgt tcctcttcat cgacaagatc cttgaacttt ccgaccgtca tgtagtcggg
      361 gtgaagagcg tcacgatgaa cgaatggttc tttcccggac attttcccgg tgcgccagtc
      421 atgccgggcg tgatccagat cgaagcgatg gcccaggtgg gaggcatttt agtgctgaat
      481 acggtcccgg atccggagaa ctacctcacg tatttcatca agatcgataa caccaagttc
      541 cgcgatatgg tggtgccggg tgatacgtta ctttttcacc ttgaattgat cagcccgatc
      601 cggcgcggca tctgtcatat gcgcggaaag gcctttgtcg gcgacaaagt ggtgatggag
      661 tctgagatga tggcgcaaat cgtgcgccga gacaagaaaa aatgaaccgg ccaactaacg
      721 aagccactgc catcgagccg aagcctatgc aagcactgaa ttggatccac ccggaggcga
      781 agatcggcaa ggatgttaag atcgatccgt tcaccgtcat ccacgaggat gtcgagatcg
      841 gtgagggcac ctggatcgga agcaacgtca ccattttccc tggtgcacgg atcggaaagc
      901 atgttcgaat attcccaggc gctgttattt ccgcggtccc gcaggacctg aagttcgcag
      961 gcgaaaagac cactgctgag attggcgatc atacgaccat ccgggagtat gtcacgatca
     1021 atcgtggtac caccgcggcg ggaaaaacgg tggtcggtgc gcacaatctg ctgatggctt
     1081 acgtccacat cgcgcacgac tgctttgtcg gcgatcattg tatcctggcc aacggcgtca
     1141 cgctggctgg acatatcgag attcacgatt atgccatcat cggcggactg tcggcgatcc
     1201 atcaattcgt acagatcgga tcgcatgtga tgatttccgg tggatccctg gtgcgaaaag
     1261 atgtgccgcc attcgtgaaa gcggcccacg agcccctcgc gtatgtcggg gtgaatgctt
     1321 taggcctgcg tcgacgtggc ttctccaacg atcagatcgc acagatacag gagatctacc
     1381 gcaaggtgtt cgtcagcgga ttaaacatta cacacgccat cgaggcagtt gaacaagaca
     1441 ttcccgcatc cgccgagcga aaactgattc ttgattttat ccgtaagtcc agccgtggtc
     1501 tgatgaaagg gtatgccgca cagagtgatg agtgatgagc gcgttcagga tcagtttaga
     1561 aaaggcaggt aagcggtatt tacgggaatg gatttttcgg ggcgtagaac tctcgctaag
     1621 ttcaggagac cgcatcgcgg tgatgggtcc gaacggctcc ggtaaatcca cgctcttgca
     1681 actgatcgca ggatatgtgc taccgacgga aggcgctgtc cggcatttcc aacacgacca
     1741 gttagtggat cctgatttgg tctatccgag tatctccatg gcggctcctt acctggaatt
     1801 gatcgaagag tttacgcttc gcgagtcgat tgcttttcat tgttccttta aacagctccg
     1861 aaacggtttg atgccggaag atgtgatccg gatcgcgaat ttgtcggatg ctgccgataa
     1921 ggtgatgaag tacttttcgt cgggtatgaa gcagcgggtc cggctcgcac tggcggtatt
     1981 gagtgatacg gatgttttgc tgctcgacga gccttgttcg aatcttgatc gggaaggtat
     2041 ccgatggtac caacagctga tcgaggagca tggagcaggg aggatcctgg tggtgtgttc
     2101 caacaacatt gccgaagaat attccttttg t
//
LOCUS       DAOTON010000521         2124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40524_length_2124_cov_2.989367, whole genome shotgun sequence.
ACCESSION   DAOTON010000521 DAOTON010000000
VERSION     DAOTON010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40524_length_2124_cov_2.989367"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="P5292_09915"
     CDS             complement(<1..670)
                     /locus_tag="P5292_09915"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRS39483.1"
                     /translation="MKQLTIAGLLLVGGLALSVSACRKDKTDEPEEAYQPTPYQFNVP
                     AGFPQPKYYESNPLTVEGVRLGRMLFYDPILSSNGKSCSSCHPQDEAFARSIHVNPAG
                     ESVSIPPVINLTFNPDFEWYGQEPVMEHVPLADFGPEFFNSNMDTIRARLSGHALYPR
                     YVYEALGISDLYAVGDEELKHQLARSIIQFIRTIVSSNSKYDRVMRHQEVFTPEESDG
                     SNIFF"
     gene            complement(677..1414)
                     /locus_tag="P5292_09920"
     CDS             complement(677..1414)
                     /locus_tag="P5292_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39484.1"
                     /translation="MNYKLGLISALTGLALLSSCKKDEEETPTPAPTSSTVTFSLQHE
                     VDGDALVYNTQWYNHPAGSYTYSVTRLFYYLSDVRLVKADSSTVLLKDFQYVDAEQSQ
                     FCSFSASSVPLDDYIGLKFNIGLDSVLNQTDALPATSENQGMEWPVPMGGGYHFMKFE
                     GYYTDTVQHGFAMHLGRNENLVSTAIWQRVAVQSTAVNFTMTMNMNEWFRNPAIYDFN
                     VDGSSSMGSMSAMMKLAMNGPDVFRLN"
     gene            complement(1536..>2124)
                     /locus_tag="P5292_09925"
     CDS             complement(1536..>2124)
                     /locus_tag="P5292_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39485.1"
                     /translation="ELLLLARILWNFLRDARQARVHWNTEARRWIFAADAWVLLNLAL
                     AILLSIPALNAFTHGTHITVAHAMGATIGINTNLLFASLAFKPNAKDQPSNELRSVSW
                     LLWSMQCLLLCFWTGLLLAGWLRIDAIRQQLTFAEMGLRLLPYYRYLGFAGWILGALV
                     GFVGWRFLRALLKPVKSVKTESEPEKEMQVVTDNG"
BASE COUNT          578 a          543 c          484 g          519 t
ORIGIN      
        1 taaagaagat attgctccca tccgactctt ccggcgtgaa cacttcctga tgccgcatga
       61 cgcggtcata ctttgaattc gaagaaacga tggtgcgaat aaactgaatg attgatcgcg
      121 caagctggtg cttcagttct tcatcaccga cggcatataa atcactaatg cccaatgctt
      181 catagacata gcggggatac agggcatgtc cgctcagtcg tgcccggatc gtatccatgt
      241 tggaattaaa aaactctgga ccaaaatccg ccaatggcac atgctccatc accggttcct
      301 gtccgtacca ctcaaaatcc ggattgaatg tcaggttaat taccggcgga atgctgacgg
      361 attcacctgc gggatttacg tggatggatc ttgcaaacgc ttcatcctgt ggatgacaag
      421 aggaacacga cttgccattg gaagacagga tcggatcata aaacaacatt cgcccgagcc
      481 ggactccttc caccgtcagt ggattgctct cgtagtattt cggctgggga aaaccggccg
      541 gaacattgaa ctgatacgga gtaggttgat acgcttcttc gggttcatcc gtcttgtcct
      601 tccgacaagc ggaaacactc agggccaatc cgccaacgag caacaaacca gcaatggtga
      661 gttgtttcat gatctattaa tttaatctga atacatcagg tccgttcatt gccaatttca
      721 tcatggccga catgctgccc atactggaag aaccgtcgac attgaaatca tagatggccg
      781 ggttccgaaa ccattcattc atgttcatgg tcatggtaaa attgaccgcg gtggattgta
      841 cggctacccg ttgccagatc gcggtgctca ccaggttctc attgcggccg aggtgcatgg
      901 cgaatccgtg ctgtacggta tcggtataat atccttcaaa cttcatgaag tgatatccgc
      961 cacccatcgg caccggccat tccatgcctt gattttcaga agtcgccggc aaggcatcgg
     1021 tctgattcaa aaccgaatcc aaaccgatat tgaatttcaa accgatataa tcatccagcg
     1081 gaaccgaaga agctgaaaag gagcagaact ggctttgctc cgcatcaaca tattggaagt
     1141 ctttcaacaa taccgtactg ctgtccgctt tcaccagtcg cacatccgat aagtaataga
     1201 acagccgtgt gacgctgtag gtataactac ccgcgggatg attataccac tgcgtgttgt
     1261 ataccaacgc atcaccatcc acttcatgct gcagggagaa agtgactgtc gatgaagtcg
     1321 gagcaggtgt cggagtttct tcctcgtcct ttttacagga ggataacagc gctaagcctg
     1381 ttaatgcact gatcaatccg agtttatagt tcatagcact cttattttgg ttattgatta
     1441 aactacatta ttcgcttcaa tccgtccatg atagcgatca gatatgaaat gatcttcctg
     1501 gataattcaa gtaaaatgag cggaattcga taccgtcagc cattatcggt tacaacttgc
     1561 atttcctttt ccggttcact ttcagttttt acgcttttca cgggtttcag tagcgctctt
     1621 agaaatctcc aacccacgaa tccaaccaag gcgccaagga tccaacctgc aaagccgagg
     1681 tagcggtaat acggaaggag acgtaaaccc atttccgcga aagtcaattg ttgccgaatg
     1741 gcatctatcc gtaaccaacc cgccagcagc aatcctgtcc agaagcacaa taacaaacac
     1801 tgcatggacc acaacagcca ggaaaccgac cggagttcat tcgaaggttg atccttcgca
     1861 ttgggtttga atgcgagtga ggcaaacaat aagttcgtat tgataccgat cgttgccccc
     1921 atggcatgag cgaccgtgat atgcgttcca tgcgtaaaag cattcagagc aggaatcgac
     1981 aataaaatcg ccagcgcaag gttcaagagc acccaagcat ccgcggcaaa aatccaccgt
     2041 cgtgcttccg tgttccaatg tactcgggct tgccgtgcat cacggagaaa attccagagg
     2101 atcctggcca ggagcagcag ctcc
//
LOCUS       DAOTON010000522         2123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40542_length_2123_cov_3.738395, whole genome shotgun sequence.
ACCESSION   DAOTON010000522 DAOTON010000000
VERSION     DAOTON010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40542_length_2123_cov_3.738395"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="P5292_09930"
     CDS             complement(<1..681)
                     /locus_tag="P5292_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39486.1"
                     /translation="MRRSLLAILLLLTGFSARANFEFNEKVSEAYTSIIRLRLDQGQQ
                     LLRDEKFRHPGNRIVILYENYIDFLKAFISENEIAFLELKKRSTQRLKQLDESSSGEQ
                     SPWYLYAKAEIIVQEALLRVKFREHVTAANQMRKAWKLIERNEALYPTFVLNRKLSGF
                     LHTVVGAVPKEYHWLVDLAGMEGTIPQGVEELNTLLRMLQGSELEPYREEIFFYLGNI
                     RLSFYSGND"
     gene            complement(873..1634)
                     /locus_tag="P5292_09935"
     CDS             complement(873..1634)
                     /locus_tag="P5292_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRS39487.1"
                     /translation="MSRDHKEFTVEEKLKALYVLQEIDSNIDKIRTIRGELPMEVADL
                     EDELAGLTTRAEHIQEEVNSLNDQVNRKKQAAKDAKELIKKYEAQQSKVKNNREFDSL
                     NKEIEYQNLEIQLSEKRVKEFAVDVVAKAALHEQTIAILNEKQAILNLKKAELDSIVE
                     ETQKEEEQLRKISEKARTIVDERLLAGYNRLRNNTRSGLAVVSIQRDACGGCFNKIPP
                     QRQLDIRQHKKVIVCEHCGRILVDPRISGDDVVAE"
     gene            complement(1925..>2123)
                     /locus_tag="P5292_09940"
     CDS             complement(1925..>2123)
                     /locus_tag="P5292_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRS39488.1"
                     /translation="ADVLVTADFKYHQFFDAEGDIIIADIGHYESEQFTGELFRDLIL
                     KNFTTFAVRLTEVRTNPVYYL"
BASE COUNT          478 a          552 c          539 g          554 t
ORIGIN      
        1 atcgttgccc gaataaaacg acaggcggat gttccccaga tagaagaaga tctcctcccg
       61 gtatggttcc agttccgatc cctgcagcat gcgcagcagg gtgttcagtt cttccacgcc
      121 ttgcggaatg gtgccttcca ttccggccag gtcgaccagc cagtgatatt ccttcggtac
      181 ggcaccgacc accgtatgca gaaaaccgga aagtttgcgg ttgagaacga aagtcggata
      241 gagcgcttcg ttacgctcga tcagtttcca ggccttccgc atctgattgg cagcggtaac
      301 atgctcccga aacttcacac gcaaaagggc ttcctgtacg atgatctccg ccttggcata
      361 caagtaccag ggcgattgct cgccggaaga ggattcatcg agctgtttta agcgttgcgt
      421 gctccttttc ttgagctcaa gaaaggcgat ctcattctcc gaaatgaacg ctttcaaaaa
      481 gtcgatgtaa ttctcataca ggataacgat acggtttccg ggatgccgga atttttcgtc
      541 gcgcagcagt tgctgaccct gatcgaggcg caaccggatg atggaagtat acgcttcgct
      601 gaccttttca ttgaactcaa agttggccct ggccgaaaat ccggtgagca gcaagaggat
      661 ggcgagcagc gaacgacgca tcggtatgca aagtaagaaa gacgtttgaa cgttgaaacg
      721 ttctaacgtt tgaacgttac aacgtttgaa cgttagaacg ttaaaacgtt acaacattca
      781 aacggttaaa cgtttaaacg ggaagttcac aagtgcctac aaatacaaaa gccggtcttg
      841 ggaaccggct ttcgtgataa gttgacctgg aatcactccg cgactacgtc gtcgccggaa
      901 atgcggggat caacgaggat acgaccgcag tgttcgcaca cgatcacctt cttgtgctgg
      961 cgaatatcga gttggcgttg cggcgggatc ttgttgaaac aaccgccgca ggcgtcgcgc
     1021 tggatgctga caaccgccag gccgctgcgc gtattgttac gaagacggtt atagccggcc
     1081 agcaatcgct cgtcgacgat cgtgcgggcc ttttcggaaa tcttacggag ttgctcttct
     1141 tccttctgcg tctcctcgac gatgctgtcg agttcggcct tcttcaggtt gaggatagcc
     1201 tgcttttcat tcaggatcgc gatggtttgt tcatgcagtg ccgccttggc aaccacatct
     1261 actgcgaatt ccttcacacg cttttcggaa agctggatct cgaggttctg gtattcgatc
     1321 tctttgttga gcgagtcgaa ttcacggtta ttcttcacct tcgactgctg ggcttcgtac
     1381 ttcttgatga gttccttcgc gtccttggca gcctgcttct tgcggttgac ctgatcattg
     1441 agcgaattca cttcttcctg gatgtgctcg gcccgggtgg tgagaccggc gagttcatct
     1501 tccaggtcgg ccacttccat cgggagttcc ccgcggatgg tccggatctt atcgatgttc
     1561 gagtcaatct cctgaaggac atacagcgcc ttcaattttt cttcgacggt gaactccttg
     1621 tgatcacggc tcatgatctc ttcgagtttg cgctccaggc gcgacacatc gatgatcttc
     1681 tcgatcttac ggggctgcag ggtggctgcc ttggtcttga tcttggcttt agcgattcgg
     1741 gaaagactca cgtcgttcta gattttagtt agcgctgatt gacaacgagc acagggaata
     1801 acatgaaaat ccccctacac tcgcccgctc acccggttcg tgatcgtaac tacttcaaac
     1861 agcagtagta gcaaatccct aaaccgattt tccgaaatgc ttccgcggtt ttcctgctgg
     1921 tactttagag gtaataaacc ggattcgtcc gcacttccgt caagcggacg gcaaaggtag
     1981 taaaattctt cagaatcagg tcccgaaaca gctccccggt aaactgttcg ctttcgtaat
     2041 ggccaatgtc ggcaatgatt atatctcctt cagcatcaaa gaattggtgg tatttaaagt
     2101 cggctgtcac cagcacatcc gca
//
LOCUS       DAOTON010000523         2121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40609_length_2121_cov_4.080348, whole genome shotgun sequence.
ACCESSION   DAOTON010000523 DAOTON010000000
VERSION     DAOTON010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40609_length_2121_cov_4.080348"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="P5292_09945"
     CDS             complement(<1..572)
                     /locus_tag="P5292_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS39489.1"
                     /translation="MRSWLFILLLTFSRLITPLPAKAEELQDLLTKLKQSRQDTAQVQ
                     LLLQIAKAYANGGVPDSNSYYAEQALRLSMLLNDQRGEALARYQLGRSQIARANYLRA
                     LEHFLEARNLFESLQDTLGQANCRMQLGVIAYTEKNYSDALTSFTQALPLYLSINELR
                     LASTVRYLSGLCRTELGRYAEAETDLMLAL"
     gene            complement(614..1039)
                     /locus_tag="P5292_09950"
     CDS             complement(614..1039)
                     /locus_tag="P5292_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39490.1"
                     /translation="MKTPHFKTALVSLLVLFAFNSLAGDPEGARTVDNPKSGGGTSIM
                     PADAARLAQSYMDAKPVNPRQAFFLSKAALDSIFKHDLTATGIMVVPVREEGDYMNLV
                     VKGYRSDKILVNTQSGNSVFLMQTYCPTECDMMNELGKE"
     gene            complement(1074..1550)
                     /locus_tag="P5292_09955"
     CDS             complement(1074..1550)
                     /locus_tag="P5292_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39491.1"
                     /translation="MKNIIIIVLSAVTAVAILEALGTIDWIDKDTVIMVLDAPKSCDN
                     GKREPAKPLEGGGISIKEDECSQYIAKFDTAKQLNKRNWFYLSKVAIDSIFSKDVAAN
                     GLSIFPVIDATDSMNIVVAPVISEHTLINASYGYASFLTQTFCPSDCDVTERMMSE"
     gene            complement(1686..2102)
                     /locus_tag="P5292_09960"
     CDS             complement(1686..2102)
                     /locus_tag="P5292_09960"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRS39492.1"
                     /translation="MTELSFLDEAPTGVTDTEDDILQETARQLTAYFNGERTSFSIPL
                     EPSGTLFQQKVWEELLRIPFGATISYRELSIRLGDPLCIRAAAAANGKNPIGILIPCH
                     RVIGSDGSLTGYAGGLWRKEKLLEIEGVLRPQPRLF"
BASE COUNT          498 a          516 c          567 g          540 t
ORIGIN      
        1 tccagggcaa gcatcagatc cgtttcggct tccgcgtaac gcccgagttc tgttcgacat
       61 aaaccggata aatagcgaac cgttgatgca agccgcaatt cgttgatgga aagataaagc
      121 ggtagcgcct gcgtaaagga tgtcagtgcg tcggaatagt tcttttccgt gtatgcgatc
      181 acccccagtt gcatccggca attggcctgc cccaacgtgt cctgcagcga ctcgaacagg
      241 ttacgcgcct ctaagaagtg ctcgagtgca cggaggtaat tggcccgggc gatctgagac
      301 cgtcccaact ggtaccgtgc cagtgcttct ccgcgctggt cgttcagcaa catgctcagg
      361 cgaagtgcct gctcggcgta gtacgagttg gagtcgggta cccctccgtt cgcataagct
      421 ttcgcgattt gcaataggag ttgtacctgt gccgtgtcct gtcgggactg cttgagtttg
      481 gttagcagat cctgtaattc ctccgctttg gctggaagcg gagtgatcag acgggaaaac
      541 gtcagcagta aaatgaacag ccaggatcgc ataagctgcg gtaaggagga ggaacggcag
      601 tgaactttga tgattattct ttgccgagct cattcatcat gtcgcattcc gtcgggcaat
      661 aggtctgcat gaggaatacg ctgttaccgg actgcgtatt gactaggatc ttgtcactgc
      721 ggtatccctt tacgacaagg ttcatgtagt cgccttcttc ccgcacgggc actaccatga
      781 ttccggttgc cgttaagtcg tgtttgaaaa tcgaatccag cgctgctttg cttaagaaga
      841 aagcctgccg cggattaacc ggttttgcat ccatataact ctgggcgaga cgcgcggcat
      901 cggcaggcat aatggatgtg ccgccacccg atttcggatt atcgaccgtg cgtgcacctt
      961 ccggatctcc ggcaagggag ttgaaggcaa aaagtaccag caaagaaacc aaagcggtct
     1021 tgaagtgggg agttttcatg gtggaagtgg ttgtgtggtt ataggttggc ggattattca
     1081 ctcatcatcc gttctgtgac atcacagtcg ctcggacaaa atgtttgggt tagaaaactt
     1141 gcgtagccgt aactcgcgtt gatcagcgta tgctcgctga tgacgggcgc tacgacgata
     1201 ttcatactgt cagtcgcgtc aattaccgga aagatggata gtccgttggc tgccacgtct
     1261 ttcgaaaaaa tgctgtcgat ggcgaccttg cttagataaa accagttgcg cttgttaagc
     1321 tgtttggccg tatcgaattt cgcgatgtat tgtgaacatt cgtcttcctt gatcgagatg
     1381 ccgccgcctt ctaaaggctt ggccggttcc cgttttccat tgtcacagga tttgggtgcg
     1441 tctaagacca tgatgactgt atccttatca atccagtcga tggtgcccag ggcttccagt
     1501 atcgccactg ccgtaacagc cgacaataca atgatgatga tgtttttcat ggaggatata
     1561 gtttgagaat gaggaatgaa caaaagcgac tactacgaaa tttaaaacgc cggtacgggt
     1621 tttcaaaata aatcttccat gccgccaaat cgttcgaaaa aggcaataat ggtagaatgt
     1681 cagccttaaa aaagccgcgg ctgaggacga agcacgcctt caatttccaa tagcttctcc
     1741 ttccgccaca aacctcctgc ataaccggtc agacttccgt cgctcccgat cacgcgatgg
     1801 cagggtatca ggatgccgat cgggtttttg ccgttggctg ctgccgctgc ccggatacag
     1861 agtgggtcgc ctagccgaat ggaaagttct cggtaggaaa tggtagcgcc aaatggaata
     1921 cgcagcaatt cttcccagac tttttgttga aacagagtcc ctgacggttc aagcgggatg
     1981 gaaaaggatg ttctttcgcc gttgaaataa gcagtcagtt gtctggcggt ttcctgcagg
     2041 atatcgtctt cagtatccgt tacaccagta ggcgcttcgt cgagaaacga tagttccgtc
     2101 aacgcatttc cattgccaga c
//
LOCUS       DAOTON010000524         2121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40616_length_2121_cov_3.310745, whole genome shotgun sequence.
ACCESSION   DAOTON010000524 DAOTON010000000
VERSION     DAOTON010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40616_length_2121_cov_3.310745"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="P5292_09965"
     CDS             <1..442
                     /locus_tag="P5292_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRS39493.1"
                     /translation="GDKSQTARQKALQGFKDRKVRVLVATDIAARGIDVDKLSHVINF
                     EIPEVAETYVHRIGRTGRAGANGVAISFCCPDEIEYLRDIQKLLKRTIPILPTPDLPA
                     PPPVTDSAGGHHRHTSRPVSKSAAPKKSANKSRHRKPAARKAHG"
     gene            complement(447..>2121)
                     /locus_tag="P5292_09970"
     CDS             complement(447..>2121)
                     /locus_tag="P5292_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS39494.1"
                     /translation="SRIALEVVRTDRRGDTLWISTLLVDTTNVPVKLLPTEDDGAFVL
                     ANCWSDNWNDAYMALLRMNSDGTVRWCRKIDASANDLANDMVRSGNELVLLSTTDYNL
                     GTYPGMLLTGLDTAGNLLWSQHHLGNLMTLPGGIAVQDDGSLAVVASQNGFGTGTPSF
                     YNGLLLLLHPDGTLRSSVSFGTYYDEDWLRIAAVPGGGWVVSGKAYFINHEWDGMLFH
                     FDADGGLLNNRVYDAGTNNGEIFRQLLVEPDGNLVVIGDKGTFDERNIFLTRIDPQLQ
                     PFITNEYPFSVNFTNYPFEVKVTSDDGYAITGDMRPPSWYRDIFLLKIDRNGRAPCQT
                     VGFDFTTRSETLEVTAAATTPGNAVPVLTAVATLRIRPLLRQQVVCESHQPLAVFSES
                     PDDSCSLCFDFTDNSINQPTSWSWEFPGATPSTSDQQAPTGICYPEAGEYTVRLTIAN
                     ATGTSVTERSISIDACDVWFIPNVITPGTDGKNDRFVIKGLKEPYELKIFNRWGNEVF
                     RSQELASTWAGEGRQDGNVSAGVYFYELKVVSGKNAGLYHGTIEVLPED"
BASE COUNT          472 a          590 c          586 g          473 t
ORIGIN      
        1 cggtgataaa tcccagactg cacgtcaaaa ggcgttgcag ggattcaaag accgcaaggt
       61 gcgcgtactc gtcgcaaccg atatcgcagc acgcggcatc gacgtcgaca agctttcaca
      121 cgtgatcaat ttcgagatac cggaagttgc cgagacctat gtacaccgta tcggccgcac
      181 cggtcgcgcg ggtgctaacg gcgtcgccat cagtttctgt tgtccggatg agatcgagta
      241 cctgcgcgac atccagaaac tcttgaagcg caccattcca atcctgccta cgcccgactt
      301 gcctgcacct ccacccgtaa ccgattcagc cggcggtcat caccgacata cgtcccggcc
      361 ggtttcgaag agtgctgcac cgaaaaagag cgcgaataaa tccaggcacc ggaaacctgc
      421 ggctcgtaaa gcgcacggtt aagtcgtcag tcttcgggca atacctcgat cgtaccgtga
      481 tacagtccgg cattttttcc ggacaccacc ttcagttcat agaagtagac accggcactg
      541 acgtttccat cctgtcggcc ttcacccgcc caggtggatg ccagttcctg tgagcggaag
      601 acttcgtttc cccaacggtt gaagatcttc aactcgtaag gttctttcaa ccctttgatg
      661 acaaagcggt cgttcttgcc atccgttccc ggtgtgatga cattcggaat gaaccagaca
      721 tcgcacgcat cgatgctgat cgatcgttcg gttacggaag ttccggtggc attggcgatc
      781 gtcaggcgga cggtatattc gccggcttcc ggatagcaaa tgcctgtcgg agcctgttga
      841 tcggaggtgg aaggcgtcgc tccggggaat tcccaactcc aactcgtcgg ctggttgatg
      901 ctattgtccg taaagtcgaa acacagacta cagctatcgt ccggcgattc cgaaaagacc
      961 gctaacggct gatgcgattc acagactact tgttgacgca acaatggccg gatgcggagg
     1021 gttgcaacgg cggttagtac cggtactgca ttgccgggag tcgttgcggc agcggtaact
     1081 tccaacgttt cggatcgggt cgtaaaatcg aatcctacgg tctgacaagg cgcccgtccg
     1141 ttccggtcaa ttttgagcag aaagatatcc cggtaccagg aaggcggtcg catatcgccg
     1201 gtgatggcat atccgtcatc ggacgtgact ttgacttcaa acggatagtt cgtaaagttt
     1261 accgagaacg gatattcatt cgtgatgaac ggctgcaatt gcggatcgat gcgcgtgagg
     1321 aagatgttgc gttcgtcgaa cgtgcctttg tcgccgatga caaccaggtt cccatcgggt
     1381 tccaccaaga gttggcggaa gatctcgccg ttgttggtgc cggcatcgta gacccgattg
     1441 ttcagcaatc ctccgtctgc atcgaaatga aagagcatgc catcccattc gtggttgatg
     1501 aaataggctt tgccgctgac gacccatccg ccacccggaa cggcagcgat gcgcagccag
     1561 tcttcgtcgt aataggttcc gaagctgaca ctggaacgaa gtgttccatc cggatgaagc
     1621 agaagcagca ggccattgta aaaactgggg gttccggtac cgaaaccgtt ttggctggca
     1681 acgacggcca gtgagccgtc atcctgaacg gcgatacccc ctggcagtgt cataaggtta
     1741 cccagatgat gttgtgacca gagcaggtta ccggcggtat ccagaccggt cagcagcata
     1801 ccggggtagg tgcccaggtt gtagtcggtg gtggaaagga ggaccagttc atttccgctg
     1861 cgcaccatgt cattcgccag gtcgtttgca ctggcatcga tctttctaca ccaacgaacc
     1921 gttccatccg aattcatccg caacagcgcc atgtacgcgt cgttccagtt gtcggaccaa
     1981 cagttggcca gcacgaaagc tccatcgtct tccgtaggca ggagttttac gggcacgttg
     2041 gtcgtatcga cgagtagggt gctgatccag agcgtatctc ctcgtcggtc ggttcgcacc
     2101 acttccaatg cgatccggct g
//
LOCUS       DAOTON010000525         2121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40618_length_2121_cov_2.951597, whole genome shotgun sequence.
ACCESSION   DAOTON010000525 DAOTON010000000
VERSION     DAOTON010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40618_length_2121_cov_2.951597"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="P5292_09975"
     CDS             <1..496
                     /locus_tag="P5292_09975"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HRS39495.1"
                     /translation="ASVLEDIAPTVQFVNQSQGYSAFQWQFGDGTVNTTDINPVHTYR
                     DTGSYTALLITVNTYGCRDTILKSIEVRPVSTLFAPNCFTPNGDGINEVFKPVFTNME
                     NIQVWVFDRWGKLLTSWDGIDGFWDGYYEGRKCQTDTYVYKIKGWGIDGKYSEWVGHV
                     SIIY"
     gene            complement(612..797)
                     /locus_tag="P5292_09980"
     CDS             complement(612..797)
                     /locus_tag="P5292_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39496.1"
                     /translation="MGSHDFYSNNFVPKTEQQLSLFEDFKRLRMLFGTPHRFAYLRSL
                     ARTRKHVRTVYPPGWKP"
     assembly_gap    930..939
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1046..>2121
                     /locus_tag="P5292_09985"
     CDS             1046..>2121
                     /locus_tag="P5292_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRS39497.1"
                     /translation="MKHPVIRIAAVYFLLTILPQTLNAQPFHYPAARKVDQKDTYFGT
                     TIDDPYRWLEDDRSEETAAWVTEENKVTEAYLSSIPFREEVRKRMTSLWNFAKSSVPF
                     KGGKQYFVYTNDGLQNQFVLKRLPAFDKPGIPFLDPNTMSSDGTINVNAAVPSKDGSL
                     LVYSIARAGSDWNEIYVKSVFTGTQMKDTIRWVKFSSISWRDKGFYYSRYPEPSESDK
                     LKGKNAGHQIYYHFVGTPQSKDKLIYQDTQHPMRNFSASITDDSRFLLVFGSEGTSGN
                     NLLARDLRFPKTEFKTLVRDFDHDVEVIDNDSIWLLARTNKDAARYKLVWINAVDPTV
                     PWKDAISEQEDVLQEATVGNNQIIV"
BASE COUNT          548 a          538 c          524 g          501 t
ORIGIN      
        1 tgcttcagta ctggaagata tcgctccgac ggttcagttc gtgaaccaat cgcagggata
       61 ctccgccttc caatggcagt ttggggacgg cacggtgaac accaccgaca tcaacccggt
      121 gcatacttac cgggatacgg gtagctatac cgccttgttg atcaccgtga acacctacgg
      181 ttgccgggat acgatcctga aatcaattga agtacgtccg gtatccacct tgtttgctcc
      241 gaattgcttt acaccgaacg gtgacggcat caacgaagta ttcaagccgg tcttcaccaa
      301 catggaaaac atccaggtct gggtattcga ccgctggggc aaattgctga ccagttggga
      361 cggtatcgac ggcttctggg acggctacta cgaaggacgt aaatgccaga ccgatacata
      421 tgtatataaa atcaaaggct ggggcattga cggaaaatac tctgaatggg ttggacacgt
      481 cagtatcatc tactaatctg ctgccggtat aggacatcca agtgcgtttt tttcatggtg
      541 tgtttgggaa gacccctggt tccttcggga attgggggtt cttccttttt tcccgggcaa
      601 cgttgtctga ttcagggctt ccagcccgga ggatagaccg ttcgcacatg cttacgcgtc
      661 cgagccagcg agcggagata ggcgaaccgg tgaggagttc caaacaacat gcgcaggcgc
      721 ttgaagtcct cgaagagcga caactgctgt tcggtctttg gaacgaagtt gttcgaatag
      781 aagtcgtgtg atcccatcag ttgattgtcc ttactgatgg caaaatatac ctggttggaa
      841 gctgattcaa ttttagttgg catggggcga atgattccgg gtgaacgtcg catcattacg
      901 ggtgaaacgg cgaaaagatg cgatttgcan nnnnnnnnng cgctattctt cttcacaatc
      961 agccggatcg tccatttccg ttaaagagtg ttagggtttc ccgagcggcg gaaagaaccg
     1021 taaattcgca agacctaaac cacctatgaa gcatcccgtt atccgcattg cggccgtcta
     1081 cttcttgttg accattctcc cgcaaaccct gaacgcccag ccctttcact accctgcggc
     1141 caggaaagtc gaccaaaaag acacctactt cggaaccacc atcgatgatc cgtatcgctg
     1201 gctggaagat gaccgttcgg aagaaactgc agcctgggta acggaagaaa acaaggtgac
     1261 ggaagcctat ctgtcgtcca ttccgtttcg cgaagaagtc cgaaaacgaa tgaccagcct
     1321 gtggaatttc gcgaagtcgt ccgttccttt caagggtggc aaacagtact tcgtttatac
     1381 caatgacgga ttgcagaatc agtttgtcct caaacgtctg ccggcattcg acaaaccggg
     1441 tatcccgttc ctggatccga acaccatgtc ttcagatggc acgatcaatg tcaatgccgc
     1501 cgtgccatcc aaagatggca gtctcctggt ctattccatt gcccgtgcag gaagcgactg
     1561 gaacgaaatc tacgtcaaga gcgtcttcac cggaacacaa atgaaggata ccatccgctg
     1621 ggtcaaattc tcctccatct cctggcgcga caaagggttt tattattccc gttatcccga
     1681 accctccgaa agtgacaaac tgaaaggtaa aaatgcagga catcagatct attatcactt
     1741 cgtgggaacg ccccagtcga aggacaaact catttaccag gatacacagc acccgatgcg
     1801 taacttcagt gcttccatta ctgatgacag ccgcttttta ctagtctttg gttcagaagg
     1861 caccagcggg aacaatttgt tggcccgtga cctgaggttt ccaaaaacag aattcaagac
     1921 gctggtccgt gacttcgatc acgatgtcga agtcattgat aatgatagta tctggctctt
     1981 ggcacgaaca aacaaagacg ctgcacgata caaattagtt tggatcaatg cagtggatcc
     2041 caccgtcccc tggaaggatg ccatttctga acaggaagac gtattgcagg aagccacggt
     2101 cggcaacaac cagatcatcg t
//
LOCUS       DAOTON010000526         2109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40915_length_2109_cov_3.221032, whole genome shotgun sequence.
ACCESSION   DAOTON010000526 DAOTON010000000
VERSION     DAOTON010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40915_length_2109_cov_3.221032"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..650
                     /locus_tag="P5292_09990"
     CDS             144..650
                     /locus_tag="P5292_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRS39498.1"
                     /translation="MRSEKRKAVFLDRDGVLNRERGDYTWLLEDFQINPGVLEALARL
                     ADAGFLLIVISNQGGIGKGLYQKSDTDYLHLHLERQLALGDVRLTEIYYCPHHPATGK
                     CLCRKPSGLMIEKAIARFQLDPTACFFIGDTERDVEAGHSAGVTTVRISPNASLIPIV
                     DRILSGTF"
     gene            726..1637
                     /locus_tag="P5292_09995"
     CDS             726..1637
                     /locus_tag="P5292_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRS39499.1"
                     /translation="MQAPTFLNFNGNITPSDQPIFTSANRAFRYGDAVFETIRLQQGE
                     ILFLDRHLERLRNSMQLLGMLPHDDLTFHNLYLSIRHLDQVNQLKGNGRIRLEVFRND
                     GGLYTPQTNHVSYLIEVTPLSHKDYRLNDHGLRIELYPDIKKPMSRLSNLKSSNALYY
                     VMAGLYKKQLGIEDCLVLNTEGRIAEAISSNIFLIRGNELFTPGLEEACVAGVMREYI
                     IEHERLNGRSVQETGITVDDLLKADEIFLTDVIHGLRWVGAFRHKRYFNTTSRKLLNA
                     IQEAQTKKVWAMLRPFLLERSKITSWK"
     gene            complement(1621..>2109)
                     /locus_tag="P5292_10000"
     CDS             complement(1621..>2109)
                     /locus_tag="P5292_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39500.1"
                     /translation="MSEINQFGTDWTALGEKATAWSQQLTETATKAKEFATAQTDMQT
                     QMANIKDEAMKTKMAETAQKATEDASKFEAMSNEWNQFKTTWDETTKQFTEWKDKIAK
                     GEINPADAVTGLADFKSKMSDAQAKIESWNTSFAEAKTSIEQNMASAGEMAKSIEATS
                     KK"
BASE COUNT          481 a          562 c          539 g          527 t
ORIGIN      
        1 cagttactac atggataaac ggggtggtga ccgggtgaac catgatgcgc atttgtgggg
       61 agcgatctat ggcatcgtct tcacagtgct gctgaagccg tcgctcctga tacatttttt
      121 cgaacaattg gtgtacttca gaaatgcgat ctgaaaagcg caaagccgta tttctcgatc
      181 gcgacggtgt gctcaaccgt gaacggggcg attatacctg gctgctggag gattttcaga
      241 ttaatcccgg ggttctggaa gcattggcac gacttgccga tgccggtttc ctgttgatcg
      301 tcatcagtaa ccagggcggt atcggaaagg gtctctatca aaagtcggat accgattatt
      361 tgcaccttca cctggaacgg caactggcac ttggcgacgt tcgcctgacg gagatctact
      421 actgtcccca tcatccggct accggaaaat gcctctgtcg aaaaccatcc ggtctgatga
      481 tcgaaaaggc catcgcccgc tttcaactcg acccgaccgc ctgctttttc attggtgata
      541 ctgaacggga cgtggaggcg gggcactccg caggcgtaac aacggtacgg atctcgccga
      601 acgcatcgct gataccgatc gtcgaccgca tcctttcggg gacgttttaa agaatcaaac
      661 gttgattaat tccggttcca tgacatccag atcgcgggtg gctgactacc tttgcatcga
      721 cagagatgca ggcgccaact tttttgaact tcaacggcaa catcacgccg tcggatcagc
      781 cgattttcac ctcggcgaac cgtgccttcc gttatggcga tgccgtattt gaaaccatcc
      841 gattacaaca gggcgaaatc cttttcttag accgccacct ggaacggtta cggaacagca
      901 tgcaactcct cggcatgctg ccgcacgatg atctgacgtt ccataacctc tacctctcca
      961 ttcgtcacct cgatcaggtc aaccaattga aggggaacgg caggatcagg ctggaagtat
     1021 tccggaacga cggaggactt tatacacccc agacaaatca cgtttcgtac ctgattgagg
     1081 tcacaccctt gtctcacaaa gactacaggt tgaacgacca cggactcagg atcgaacttt
     1141 atccggacat caaaaaaccg atgagccggc tatcgaacct gaagtcatcc aatgccttgt
     1201 actatgtcat ggccggattg tacaaaaagc aattgggtat cgaagattgc ctggtcctga
     1261 ataccgaggg taggatcgcg gaagccatct cctccaacat tttcctgatc aggggaaacg
     1321 aattgttcac gcccggtctg gaagaagctt gcgtagccgg cgttatgcgg gaatacatca
     1381 tcgagcacga gcgactgaac gggcgctccg tgcaggaaac cggcatcacg gtcgatgacc
     1441 ttttaaaagc cgacgaaatc tttctcacgg acgtgatcca cggcctgcga tgggtggggg
     1501 ctttccgaca caaacgttat ttcaacacga cttccaggaa gctcctcaac gcgatccagg
     1561 aagcacaaac aaaaaaggtc tgggcaatgc tcagaccttt tttgttggaa cgatctaaaa
     1621 ttacttcttg gaagtagctt cgatgctctt agccatttca ccggcagaag ccatgttctg
     1681 ctcgatgctg gtcttagctt cagcgaagga ggtattccag gactcgatct tggcctgagc
     1741 atcgctcatt ttcgatttga aatcggccag tccggtcaca gcgtcagccg ggttgatttc
     1801 acctttggca atcttgtctt tccactcggt gaactgcttg gtggtctcgt cccaggtggt
     1861 cttgaactgg ttccactcgt tagacatagc ctcaaacttc gaagcgtctt cggtagcctt
     1921 ctgagcggtt tcagccatct tggtcttcat cgcttcgtcc ttgatgttgg ccatctgggt
     1981 ctgcatgtcg gtctgagccg tagcgaattc tttcgccttg gtggccgtct cggtcagttg
     2041 ctggctccag gcggtagctt tttcgccgag tgccgtccag tcggtaccga attggttgat
     2101 ttccgacat
//
LOCUS       DAOTON010000527         2108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_40946_length_2108_cov_2.683877, whole genome shotgun sequence.
ACCESSION   DAOTON010000527 DAOTON010000000
VERSION     DAOTON010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40946_length_2108_cov_2.683877"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /gene="xdhA"
                     /locus_tag="P5292_10005"
     CDS             complement(<1..1369)
                     /gene="xdhA"
                     /locus_tag="P5292_10005"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HRS39501.1"
                     /translation="MRNIIRFFFQNNTVEIDLNRENLSPTTTVLQWLRSRPDTKGTKE
                     GCAEGDCGACTVVIGELSDDDTIRYTAIDSCLVFLPMLDGKQLITVECLSNHPQTKDD
                     LHPVQQAMVDTDGSQCGYCTPGFIMSLFALYKQGDNPDRTETCDALTGNLCRCTGYRP
                     IAEAAARSCAAPAADTIASQEPATIAALRELAASANPLLIQTSTQRYLRPMSLEAALA
                     SIAEDASLLPFNGATDLALRVTKRHEVLPALLDLSGINALRRIEILPDNIRIGAGCSL
                     ESIRRTVRDQLPALAAILDVFGSRQIRSLATIGGNLGSASPIGDTLPLLFAYGASIHL
                     ASTQGERDIPMDRYITGYRQTVRRPDELITALTIPLPGPDDLVRAYKVSKRKDLDIAT
                     VSGAFRLRIVGDTVQEAVIAFGGMAASTQRVFPAEDLLIGKPWSRTNAQAAAEVVYHH
                     FTPLSD"
     gene            1919..>2108
                     /locus_tag="P5292_10010"
     CDS             1919..>2108
                     /locus_tag="P5292_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39502.1"
                     /translation="MTTRRLLIGLIFTSFLGLLLLDASRNEVQSKENGAPAGYTGCAS
                     DYSGRTCDNCHSDYSVASV"
BASE COUNT          456 a          537 c          629 g          486 t
ORIGIN      
        1 cgtcggacaa gggcgtgaaa tggtggtaga cgacttcggc ggcagcttgc gcattcgttc
       61 tggaccaggg tttaccgatc agcagatctt ccgcggggaa aacccgctgc gtggatgcgg
      121 ccataccgcc gaaggcgatg acggcttcct gcaccgtatc gccgacgatg cgaagccgaa
      181 aagcgccgct cactgtagca atgtcgaggt ctttccgttt ggaaacttta taggcacgaa
      241 caaggtcgtc gggtccgggc aaggggatgg tgagtgcagt gatgagttcg tcgggtcggc
      301 gaacggtttg ccggtaaccg gtgatgtaac ggtccatggg gatgtcgcgt tcaccctgtg
      361 tgctggccag gtgaatggaa gctccgtagg cgaataacaa gggtaatgtg tcaccgatgg
      421 gcgaagcaga accgaggttg ccgccgatgg tggcgaggct gcggatctgg cgagagccga
      481 atacatcgag gatagctgcg agtgccggaa gctgatcccg cacggtccgg cgaatggatt
      541 cgagcgaaca gcccgcgccg attcggatgt tgtcggggag gatttcgata cggcgaagcg
      601 cattgattcc ggaaagatcc agcaaggccg gcaacacctc gtgacgtttg gtgacacgaa
      661 gcgcgaggtc ggtagcgcca ttgaacggaa ggagcgacgc gtcttccgcg atggatgcca
      721 gggccgcttc gaggctcatg ggtcgcaggt accgctgagt ggaagtctgg atgagcagtg
      781 gattcgctga ggcagccagt tcacgcaagg ccgcgatggt agctggttct tgcgaagcaa
      841 tcgtatcggc tgccggtgcg gcacaggagc gcgccgctgc ttcagcgatc gggcggtatc
      901 cggtacagcg gcataggttg ccggtcagcg catcgcaggt ttccgtacga tccgggttgt
      961 cgccttgctt gtacagcgca aacaaggaca tgatgaagcc gggagtgcag tagccgcatt
     1021 ggcttccgtc ggtatcgacc atggcttgct gcaccggatg taagtcatcc ttcgtctgcg
     1081 gatggttgga gaggcattcg acggtgatca gttgtttgcc gtccagcatg ggcaggaaga
     1141 ccaggcagga gtcgatagcc gtgtatcgga tcgtatcatc gtcggacagt tcgccgatca
     1201 caacggtaca cgcgccacag tcaccttccg cgcagccctc cttggtgcct ttcgtatccg
     1261 gcctggaacg gagccactgc aggacggtgg tagtgggaga caggttttcc ctgttcagat
     1321 cgatctcgac cgtgttgttt tgaaaaaaga agcgaatgat gttcctcaat gttgatgaca
     1381 ggtataggca gtaaatttaa ggtattcggt caaactttta ctgctatcca ggttgcgctt
     1441 agcgtgcagt tgtttttaaa aagcgtctcg cttggacatt cagatgcagg cgttttcaaa
     1501 atccaccatc ggaaaattgt ttgttacatg aaatgaacag ctgttgatac taatcagtgc
     1561 tccagtatat ccttgcaaac gactgatagt agcgaacttc cggtattatc cctgaatagt
     1621 atcatccatc cttcttctcc tttcatggat catacaagat tgcgtgtttc tttcgttgac
     1681 gaatactgaa cgagaaggaa cgcgacgcgt gatcggtttt actgatcccg gtccggaaca
     1741 gctgtaactg cgacgattac tgttccggcc gccaccaacg cgccgtcatt ttccaccccc
     1801 gaatgggttt cttccgagca gtggtcatct ctgcaattta cccccgcgct gcgcacgaca
     1861 ggctgttttc ctgtcgctga agttgtctta ccctatacta acccataaca ccatcactat
     1921 gacaacacgg cgtttattga tcggattgat cttcacctca tttctcgggc tgctgctcct
     1981 ggatgccagc aggaatgaag ttcaatccaa ggaaaatggc gcaccggccg gatataccgg
     2041 ttgcgcaagc gattacagtg gtcggacctg cgacaactgc cacagcgact attcggttgc
     2101 atccgtca
//
LOCUS       DAOTON010000528         2105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41023_length_2105_cov_2.643415, whole genome shotgun sequence.
ACCESSION   DAOTON010000528 DAOTON010000000
VERSION     DAOTON010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41023_length_2105_cov_2.643415"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="P5292_10015"
     CDS             <1..224
                     /locus_tag="P5292_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HRS39503.1"
                     /translation="YLSQLKRGQKDVVERFTDYALSLKLLEMGCIPGEVVEVLRIAPF
                     GDPIAIAVSGYQLSLRKDEAATVRVRLIS"
     gene            254..>2105
                     /gene="feoB"
                     /locus_tag="P5292_10020"
     CDS             254..>2105
                     /gene="feoB"
                     /locus_tag="P5292_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRS39504.1"
                     /translation="MKIALIGNPNSGKSTVFNALTGLNQKVANFPGVTVDKRTGTTVI
                     RSESGEAVNCEFIDLPGTYSLYPKSPDEQIPFQLLCNPDHELHPDLSIVVADGTNLKR
                     NLFLCSQLIDLRIRVILVINMMDIVRARGTDIDLDCLSKKLGVTIVPMNARRSDGLEE
                     LKRAIVSAQPVSNAFFLDTRAFAPQLVDRIREEVRVNSDYNAFLVANNLGLIETFALP
                     EFKREKIREACQQFGYDPQQMQARESLERYKAITNILQECVRSEEHPVRKSGTYKIDS
                     ILTHRVFGYLIFLFILFLIFQAIFAWATYPMDLIDRGFATLSQRLHDNMAPGLLTDLL
                     IDGILAGLNGIVVFIPQIALLFFFVAILEDSGYMARVSFLMDRLMRRFGLNGKSMIPL
                     MSGVACAVPALMSTRTIQNRKERLITILVTPLMSCSARLPVYTLLIALVIPNDLMLGM
                     SMQGLVLMGLYLLGFVSALFAALVFKYMLRSKERSFFVMEMPLYRLPRFSTVFLHILE
                     KVKIFLFDAGKIIIAISIVLWFLSSFAPGNAFEQIEATYRTEKMRQLLPAEELEHRIQ
                     TEKLETSYAGRLGHFIEPVIKPLGFDWKIGIALITSFAAREVFVGTMSTIY"
BASE COUNT          441 a          648 c          551 g          465 t
ORIGIN      
        1 gctacttatc ccagctgaaa aggggccaga aagacgtcgt ggagcgtttc accgactacg
       61 cgctttcgct taaactcctt gaaatgggat gcatcccggg agaggtggtg gaagtgctgc
      121 gcatcgctcc tttcggcgac cccattgcca ttgccgtttc gggttatcaa ctcagcctgc
      181 gaaaggacga agccgccacg gttcgcgtcc gattgatctc ctgaagagta ccttacagcc
      241 cttgtcgaac aacttgaaaa tagccttgat cggtaacccc aattcgggga agagtaccgt
      301 attcaatgcc ctgacagggc tgaaccaaaa ggtcgccaat ttccccggtg tcacagtcga
      361 caagcgcacc ggcacgaccg tcattcgcag tgaaagcgga gaagccgtga actgcgagtt
      421 cattgatctt cccggtacgt attcgcttta ccccaagtcg cccgacgaac agatcccgtt
      481 ccaattgttg tgcaacccgg accacgaact gcacccggac ctgtcgatcg tcgtagccga
      541 cggcaccaac ctgaagcgca acctcttcct ttgctcgcaa ctgatcgacc tgcgtatccg
      601 cgtgatcctg gtgatcaaca tgatggatat cgtgcgcgcg cgcggaaccg acattgacct
      661 ggattgtctt tcgaaaaaac tgggcgtgac catcgttccg atgaatgcac ggcgcagcga
      721 cggcctggaa gaactgaagc gtgcgatcgt atccgcccag cctgtctcga atgccttctt
      781 cctcgacacg cgcgcgttcg ctccgcaact ggtggatcgc atccgcgagg aagtgcgcgt
      841 caacagcgat tacaacgcct tcctcgtagc caacaacctc ggactgatcg agaccttcgc
      901 gctgccggag ttcaaacgcg aaaagatccg cgaagcctgc cagcagttcg gctacgaccc
      961 gcaacagatg caggcacggg aatcgctgga acgatacaag gcgatcacca acatcctgca
     1021 agaatgtgta cgatcggaag aacatccggt ccgcaaatcg ggcacataca agatcgactc
     1081 catcctcacc caccgggtgt tcggctatct gatcttcctg ttcatcctct tcctcatctt
     1141 ccaggccatc ttcgcctggg cgacctatcc gatggacctc atcgaccggg gcttcgcgac
     1201 cctgagccag cggctgcacg acaacatggc gccgggtctg ctgaccgatc tgctcatcga
     1261 cggcatcctc gccggcttga acggtatcgt ggtcttcatc ccgcagatcg ccctgctctt
     1321 tttcttcgtc gccatcctgg aagattcggg ctacatggcg cgggtctcgt tcctcatgga
     1381 ccgtctgatg cggcggttcg ggctcaacgg gaaatccatg atcccgctga tgagcggtgt
     1441 ggcttgtgcc gtaccggcgc tgatgagcac ccgcacgatc cagaaccgga aagagcggct
     1501 gatcacgatc ctcgtgacgc cgctgatgag ttgctccgcg cgtttgcccg tctatacact
     1561 cctgatcgcc ctggtcatac cgaatgatct catgctgggc atgagcatgc agggactggt
     1621 gctcatgggt ctttacctgc tcggtttcgt atctgctctt ttcgcggcct tggtcttcaa
     1681 gtacatgctg cgttcgaagg aacgcagttt cttcgtgatg gaaatgccgc tgtatcgctt
     1741 accgcgcttc tcgaccgttt tcctgcacat cctggaaaag gtgaagatct tcctctttga
     1801 tgccggtaag atcatcatcg cgatctccat cgtgctctgg ttcctttcct ccttcgcgcc
     1861 cggtaacgct tttgaacaaa tcgaagcaac ctaccggacc gagaagatga gacaattgct
     1921 acccgcggaa gagctcgaac accggatcca gaccgagaag ctggaaacct cctatgccgg
     1981 tcgcctcgga catttcatcg aacccgtcat caagccgctg ggcttcgact ggaagatcgg
     2041 catcgcgctc atcacctcct tcgcggcgcg ggaggtattc gtgggaacca tgtcgacgat
     2101 ctaca
//
LOCUS       DAOTON010000529         2104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41039_length_2104_cov_2.960957, whole genome shotgun sequence.
ACCESSION   DAOTON010000529 DAOTON010000000
VERSION     DAOTON010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41039_length_2104_cov_2.960957"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1522)
                     /locus_tag="P5292_10025"
     CDS             complement(50..1522)
                     /locus_tag="P5292_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRS39505.1"
                     /translation="MVQRYLFSVLLFALVSMMTRAEQPRSESRTVPNRHLVSGNPEPP
                     SPARAVRVDVSSGECLSLADMEQMKVDIRTNIERLKLQRPGLLQRRSQVHPLFAWPVR
                     SKAGFSDYGYYTVQNFVDHDWGFPNLLEDWNCGMRTYDWGTGNHAGTDIILWPYPWRR
                     MDEQVMEVVAAAPGVIINKVDGNFDRQCVNNGTGVWNAIHLLHADSSQSWYLHFKSGS
                     LTTKGVGDYVSEGEYLGTAGSSGSSNWPHLHFQVLDNTGALIDPYQGPCNNFNPDSWW
                     QSQPDYIIPRINRILTKSTTTEYYSCPDPEITYEKDTFAWGDSLVLQLYYRDLDNNAR
                     TTLRITDPNGVQQITWDFDSPWLFGATTWVQWYYVLNNWWTPGTWTFAADFGGNSYAH
                     SFVIEAPVGMDPAEPLLDFIMSPNPAAERLMMSFQSPKNQNLELKILDATGRCIVNRT
                     LEVVAGKQTVEQPIDGLSAGIYQLQLAGNGKSLSRKLIVR"
     gene            complement(1611..>2104)
                     /locus_tag="P5292_10030"
     CDS             complement(1611..>2104)
                     /locus_tag="P5292_10030"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRS39506.1"
                     /translation="MKTILLRYVTYNQWANDRLLSFVSENFKPEQLEQSIVSSFPSVR
                     TTLLHLWGAECIWLMRLRGESPTTWKWMEFSGSFQELYGEIIENDKAWIDFIETSGDA
                     FLQTQFRYKSLDGTEYHNMVAEAVLHCMNHSTFHRGQLITLFRQLGFKKLFSTDLIAF
                     SRD"
BASE COUNT          504 a          551 c          575 g          474 t
ORIGIN      
        1 ggtaccgatc actgcgcaag gttgggatgc gctgctatcg aagaactgat cagcgtacga
       61 tcagctttcg gctgagcgat ttgccattgc ctgccagttg caattgatag atgccggccg
      121 acaagccatc aatcggctgc tccaccgttt gcttaccggc gacgacctca agcgtacggt
      181 ttacaataca tcgtccggtc gcatcaagga tcttcaattc aagattttga ttcttaggag
      241 actggaaact catcatcagt cgttctgcgg caggattcgg actcatgata aaatccaaca
      301 agggctcagc cgggtccatt cctaccggcg cctcaatcac aaaggagtga gcatacgaat
      361 tcccgccgaa gtctgcggcg aaagtccagg tacccggcgt ccaccaattg ttgaggacat
      421 agtaccactg gacccaggta gtcgcaccga aaagccacgg gctgtcgaag tcccacgtga
      481 tctgttgtac gccattggga tccgtgatac ggagcgttgt tcgtgcattg ttgtcgaggt
      541 cccggtaata gagttgcagg acgagcgaat cgccccaggc aaaggtgtct ttctcgtagg
      601 tgatttcggg gtcgggacag gaatagtatt ccgtggtagt ggatttcgtc aggatcctgt
      661 tgatgcgtgg aatgatgtaa tccggctgtg actgccacca ggaatccggg ttgaaattgt
      721 tacagggacc ctggtaggga tcgatcagtg caccggtgtt atccagaacc tggaaatgca
      781 ggtggggcca attggaactg ccggagctgc cggccgttcc gaggtactcg ccttccgaaa
      841 cataatcgcc cacacctttt gtcgtgagtg agccggactt gaaatggagg taccacgact
      901 gcgaactgtc ggcatgcaga agatgaatgg cgttccagac tccggtacca ttgttcacgc
      961 actgccggtc gaaattaccg tcgaccttgt tgatgatgac gccgggagca gccgcaacaa
     1021 cttccatcac ctgttcgtcc atcctccgcc agggataggg ccaaaggatg atatcggttc
     1081 ctgcatggtt gccggtcccc cagtcgtatg tacgcatacc gcaattccag tcctccagga
     1141 ggttgggaaa tccccaatca tgatcgacga agttctggac ggtatagtaa ccataatcgg
     1201 aaaaaccggc cttggatcga accggccagg cgaaaagcgg atgaacctgc gaacgccgct
     1261 gcagaaggcc ggggcgttgc aacttcaaac gctcgatgtt cgtccggata tcaaccttca
     1321 tctgttccat atccgctaag gacaggcact cgcctgacga cacatcgacg cgtactgcac
     1381 gggccggaga tgggggctcg gggttgcccg agaccagatg tcgattgggg accgtgcggg
     1441 attcacttcg cggttgttcc gcccgtgtca tcatggatac caaagcgaag agcaatacgg
     1501 agaacaggta gcgttgcacc atgggagcgt actttattac gattggagaa atctttagtc
     1561 gaactaaaat acgaaaatca tcaaaattcg gcaaagcctt tacagtggct ttaatcccgg
     1621 gaaaacgcga tcaaatccgt cgagaacagt tttttaaaac ccaactgccg gaacaatgtg
     1681 atcaactgac cacgatgaaa ggtgctgtgg ttcatgcaat gcagcacggc ttcggcgacc
     1741 atgttgtgat actccgtacc gtccagcgac ttgtaccgga attgggtctg taaaaatgca
     1801 tccccgctgg tttcgatgaa atcgatccag gctttgtcgt tctcgatgat ctcaccgtac
     1861 agttcctgaa aactaccgct gaattccatc cacttccagg tagtcgggga ttcgcccctc
     1921 aggcgcatca gccagatgca ttccgcgccc cacaggtgca acaaggtcgt ccgcaccgac
     1981 gggaagctgc tcacgatgga ctgctccagt tgttccggtt taaaattctc cgagacaaac
     2041 gacaagagcc ggtcgttggc ccactggttg taggtgacgt agcgaaggag gatggttttc
     2101 atat
//
LOCUS       DAOTON010000530         2103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41064_length_2103_cov_2.511719, whole genome shotgun sequence.
ACCESSION   DAOTON010000530 DAOTON010000000
VERSION     DAOTON010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41064_length_2103_cov_2.511719"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2103)
                     /locus_tag="P5292_10035"
     CDS             complement(<1..>2103)
                     /locus_tag="P5292_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39507.1"
                     /translation="ATFTVSGFPFPTGQFCWTPSPADVRPTPYTFTVTVTDDACPNVG
                     FQTYSFQVFVNSPLYTATGTDISCNGANDGVATVVPGSGSLTYVWSPGGQTTASISGL
                     AAGTYTVTITDTVTGCVGQSIVTINDPAVLTVATATTNPPCAGSNSGIAVATPAGGTP
                     GFSYSWNTVPPTLNDTATGLAPGTYIVTVTDSRNCTATANATINPSAPAVNVNTVSVT
                     DLQCNGDANGTATVAASGGTPSYTYSWNTVPPQFGTTATGLSGGTYIVTVTDSLGCVG
                     TDTVNIDEPAAIVIAGSSTQSTCGASDGTASASASGGTPFSFGYQYLWSVGGQTTTTI
                     TGLAAGAYTVTVTDSNGCTSSLPVTVSDSNILPPIASAIQSVLCNGQTNGIAVAVPQN
                     GTPPFTFSWNTTPVQTNDTAFNLGAGIYAVGITDANGCSSFDTVQITEPAPINLTVLS
                     SDASCYNDSSGIGVVSIVNGGTPNYTYDWQPYGGTNASVTNLIAGNYTVTVTDANGCT
                     GTSAISISEPSQITVTVNSTIEPTCYGGSDGSIDISVSGGTGGFDYFWNPGGATSQDL
                     SSVDAGIYIVTITDDNGCTFQQTVNLNQPSPVNAFAGNDTALCVGNSIQLQATLGAGM
                     TGQWTSSTGASFVNATNPTTVANNLQPGFNSLTWTVTDADGCSGLDELTIFNYTNIAA
                     NAGSDTAFCGLGFVQLNAT"
BASE COUNT          474 a          536 c          652 g          441 t
ORIGIN      
        1 gtggcattca actgcacaaa acccagtcca cagaaagctg tgtcactgcc agcgttggcg
       61 gcgatattcg tgtagttgaa aatggtcagt tcatccaatc cggaacaacc atcggcgtcc
      121 gtgacggtcc aggtcagact gttgaagccg ggctgtagat tattggcgac ggtagtcggg
      181 ttggtggcat tgacaaagga tgcaccggtt gaggacgtcc attgcccggt catgcctgca
      241 cctaaggtcg cttgcaactg aatgctgttc cctacacaca aggcggtatc attgcccgcg
      301 aaggcgttca ccggcgaagg ctgattcagg ttgaccgttt gctggaaggt gcagccgtta
      361 tcgtcggtga tcgtcacgat atagatgccg gcatcgaccg aagacagatc ctgagaggtg
      421 gctcctccgg gattccagaa ataatcaaag ccaccggtgc cgccgcttac ggaaatatca
      481 atcgagccat ccgaccctcc gtaacaggta ggctcgatgg tgctgttgac ggtcacggta
      541 atctggctgg gctcgctgat gctgatggca ctggtaccgg tgcaaccgtt ggcatcagtg
      601 acggtgaccg tgtaattgcc ggcgatcagg ttggtaacgg aggcattcgt gccgccataa
      661 ggttgccagt cgtaggtgta attcggtgtg ccgccattca cgatggagac aacaccgatt
      721 ccactgctgt cattataaca ggatgcatcc gacgaaagca cggtcaggtt gatcggggcc
      781 ggctcggtga tttgtaccgt atcgaatgaa gaacaaccat tggcatccgt aatacccaca
      841 gcgtaaatac ctgcgccgag gttgaaggcc gtatcattgg tttgtacggg tgtcgtattc
      901 caggagaagg taaagggcgg agtgccattc tgggggaccg ctaccgcgat accgttggtc
      961 tgaccgttac acaaaaccga ttgaatcgcg ctggcgatgg gcggtaatat gttgctatcc
     1021 gaaacggtaa ccggcagtga gctggtacag ccgttggaat ctgttacggt aacggtataa
     1081 gcaccggcgg ccagacccgt gatcgtggtg gttgtctgcc ccccgacgga ccatagatac
     1141 tgatatccaa aagagaaagg cgttccgcca ctggccgacg cgctggccgt tccgtccgac
     1201 gcgccgcagg tggattgcgt ggaagaaccc gcaatgacaa tggctgccgg ttcatcgatg
     1261 ttcacagtat cggttccgac acatccaagg ctgtcggtca ccgtcacgat ataggtgcct
     1321 cccgaaagcc cggttgccgt tgttccgaat tgaggaggca cggtattcca gctatacgta
     1381 taggaaggag ttccaccgct tgcagcgact gtggcggttc cgttggcgtc accgttacac
     1441 tgaagatcgg taacggaaac cgtattgaca ttcaccgccg gtgcggaagg attgatcgtg
     1501 gcattggcgg tggccgtaca atttcggctg tcggtcaccg tgacgatgta ggtaccggga
     1561 gccaggccgg tggcagtatc gttgagcgtc gggggaaccg tattccagga ataggagaag
     1621 ccgggagtgc caccggctgg agtagcaacg gcgataccac tgtttgaccc tgcacacgga
     1681 ggattggtcg tagcagttgc cacggtcagc accgcgggat cattgatggt gacaatagac
     1741 tgacccacgc agccggttac cgtatcggtg atggtaaccg tataggtacc ggcagcaagg
     1801 ccggagatgc tcgcggtcgt ctggccaccg ggcgaccata cataggtcag ggatccggaa
     1861 ccgggcacta cggtcgcgac gccgtcattg gctccgttac aggaaatatc cgttccggtc
     1921 gccgtataga gtggcgaatt aacgaagacc tggaacgagt aggtttggaa acctacgttc
     1981 ggacaagcgt catcggtcac cgttaccgtg aacgtatagg gagtcggacg aacatcggcc
     2041 ggtgagggag tccagcagaa ctgaccggtc gggaaaggaa atccgctgac ggtaaaggtt
     2101 gca
//
LOCUS       DAOTON010000531         2101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41108_length_2101_cov_2.494624, whole genome shotgun sequence.
ACCESSION   DAOTON010000531 DAOTON010000000
VERSION     DAOTON010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41108_length_2101_cov_2.494624"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="P5292_10040"
     CDS             complement(<1..304)
                     /locus_tag="P5292_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HRS39508.1"
                     /translation="MSKQHIRFIINPHSGTSKKHNLPDMIRAAIDPGRFEPEIVFTQA
                     QLHATELAREAVSMGYRAVIAVGGDGSVNETAAGLSGTTTALGIIPTGSGNGLARHL"
     gene            complement(301..>2101)
                     /locus_tag="P5292_10045"
     CDS             complement(301..>2101)
                     /locus_tag="P5292_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRS39509.1"
                     /translation="TSSGTLLLSFFYGLRFDATAICILNTPFFLFHVLLPDRWLAHGF
                     MRKILSVYFVTLNALFLLAACVDLGLYSFSARRMTTDMVRIMGFGDDFVNTVPRMVVD
                     YWYLLLLFFGLIFILYRILRWSRTTSRFYRLNEALSKRWWNLVFRMLALVLLVIGFRG
                     GLQYRPLSIGNAAQLGGGHTSALILNTPFTIIKTWGKDELERAQWMDDATARQLQPFA
                     YHPLPADSASHRPNVVILLLESFGSEYIQSLHPDGVAYTPFLDSLFKHSLLCTNAFAN
                     GKRSIEGIPAVIAGIPALMNEPFITSVYAGNRYDGLGTLLKRIGYQSVFFHGGTNGTM
                     GFDHFAKAAGYDRYFGRREYGHDEDFDGNWGIYDGPFLQRAVHEMDRLKEPFLSTIFT
                     LSSHHPYSIPPAIKSQFPEGPLPIHASVRYTDACLRDFFKTAARSTWYSNTVFIITAD
                     HTAEAETPYYLSKAGMYAIPIAFYHPDESLKGRYERTTQQIDILPSILDQVRYPDPAF
                     AFGNSIFRTGVSSGAYTCLQSVYQLIDNGYVLSMDTTVHRQLYRLQEDPSLQQDISVS
                     DSSRADRMSLRLKAMIQRFNSVLLDNAMSQSKE"
BASE COUNT          530 a          510 c          603 g          458 t
ORIGIN      
        1 ccaggtgcct ggccaggccg tttcccgaac cggttgggat gatgcccagg gcggtggtgg
       61 tgccggacaa tccggccgct gtttcattca cggagccgtc cccgcccaca gctatgaccg
      121 cacgataacc catggagacg gcttcacggg ctagctcggt ggcatgaagc tgcgcttgcg
      181 taaagacgat ctccggttcg aagcgacccg ggtcgatcgc cgcacggatc atgtccggca
      241 ggttgtgttt ttttgacgtg ccggaatgcg ggttgatgat gaatcgaatg tgttgcttac
      301 tcattcttta ctctggctca tggcattgtc gagtaataca ctgttgaagc gttggatcat
      361 ggctttcaac cgcaggctca tccgatcggc ccggctgctg tcggagacgc tgatatcctg
      421 ctgcagggaa ggatcttcct ggagccggta caactggcgg tggacggtag tgtccatcga
      481 aagtacatat ccgttgtcaa tcagttgata aacggattgg aggcaggtgt aggcgccgga
      541 ggagacaccc gtccggaaga tgctattgcc aaatgcaaat gcaggatccg gataccgaac
      601 ctggtccagg atactcggga ggatatcaat ttgctgagtg gtgcgttcgt accgtccttt
      661 caggctttca tcaggatgat aaaacgcgat cggtatggca tacatacctg ccttgctgag
      721 gtaataggga gtctcggctt ctgcggtatg atccgctgtg atgatgaaca ctgtgttgga
      781 ataccatgta ctcctcgctg cagttttaaa gaaatcgcgc agacacgcgt cggtataccg
      841 gacactggcg tgaatgggta acggtccttc cggaaattga gactttattg caggaggaat
      901 ggaataggga tggtgcgagg ataaagtgaa aatagtcgac agaaaaggct ctttcaatcg
      961 atccatctca tgaacggctc tttgcaggaa aggcccgtca taaatgcccc agttgccgtc
     1021 aaagtcctcg tcatggccgt attccctgcg accgaaatag cggtcatatc cggcggcctt
     1081 agcaaaatgg tcgaatccca tggtaccatt cgtcccgcca tgaaagaaaa cggattggta
     1141 cccgatacgt tttaagagcg taccaagtcc gtcatatcgg ttgccggcgt atacggaagt
     1201 aatgaacggt tcgttcatca gcgccggaat accggcgatc acggccggta tgccttcaat
     1261 ggagcgcttg ccattcgcga acgcgttcgt gcaaagcaga ctgtgtttga aaagcgaatc
     1321 aagaaatggc gtgtaagcga cgccatcggg atggagcgat tggatgtatt cgctgccaaa
     1381 gctctccagg aggaggatga ccacattcgg acgatgggat gcactatccg ccggaagcgg
     1441 atgatacgcg aatggttgta actgccgggc cgtcgcgtca tccatccatt gggctctttc
     1501 cagttcatct ttcccccagg tcttgataat ggtaaatggc gtattgagga tgagggccga
     1561 cgtatgacct ccacccaatt gagcggcatt gccaatactt aacggacgat attgaagtcc
     1621 tccgcgaaaa ccaatgacca gcaagacgag cgccagcatc cggaagacga ggttccacca
     1681 gcgtttggaa agtgcttcgt tgagccggta aaagcgcgat gttgtgcgag accaacgcag
     1741 gatgcgatag aggatgaata ttaatccgaa gaagagcagg agcagatacc aataatccac
     1801 caccatccgg ggtacggtgt tcacaaagtc gtcgccgaaa cccatgatgc gtaccatatc
     1861 ggtcgtcatc ctccttgcag agaagctata caatccaaga tcgacacaag ccgcgaggag
     1921 aaagagcgca ttcagggtga caaagtagac gctgaggatc tttcgcataa aaccgtgcgc
     1981 aagccagcgg tcgggaagga gaacatggaa aagaaaaaaa ggcgtattca ggatgcagat
     2041 ggccgtcgca tcaaagcgta aaccgtagaa gaatgaaagg agcagcgttc ctgatgatgt
     2101 c
//
LOCUS       DAOTON010000532         2097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41201_length_2097_cov_2.824682, whole genome shotgun sequence.
ACCESSION   DAOTON010000532 DAOTON010000000
VERSION     DAOTON010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41201_length_2097_cov_2.824682"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(405..1232)
                     /locus_tag="P5292_10050"
     CDS             complement(405..1232)
                     /locus_tag="P5292_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRS39510.1"
                     /translation="MDRILHFFETLQAHPLQRMGILIGGLLFFWILEGAIPLLPLRFR
                     KTRVRHAGVNLFFTFLHLIIHGLLAFVIILISDWCRQHQFGLVYWLDAGIGLTVLVSF
                     LTLDFFVGWLVHITQHKVPFLWRFHIIHHADTNVDATTGLRHHPAESLFRGLFFFAGV
                     WISGAPIVAVMFYQTVLVFATAFTHANIRLPDWLDNAIGYVLVSPNMHKVHHHWKQPY
                     TDSNYGAVLSVWDRLLGTLTALQPSEIRYGLDRNFPVERDEDLGTLLKAPFRKLPTD"
     gene            complement(1258..1983)
                     /locus_tag="P5292_10055"
     CDS             complement(1258..1983)
                     /locus_tag="P5292_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS39511.1"
                     /translation="MQVRFFDLSTAEAGSHYQWTYNGITDTSRHPELSFPDAGTYSVR
                     LEVTTPHGCSSYFEAEAVTVDPLPVADFNVSTEETTILTPVIRLTDASSDASAWAWDF
                     GDAAGISSDQDPVYTYADTGTYVIRLIALSPHGCRDTAYARVRVNEDYALYVPNTFTP
                     NGDGKNELFYAYGIGVHAFELLVFDRWGKVIFRSDSLQQGWNGNYQGGESLCPEGVYV
                     YKIRAQDPKGEWREASGNVNLIR"
BASE COUNT          500 a          578 c          616 g          403 t
ORIGIN      
        1 cgttgcataa atatgggaat gctgcaaagg taagcgtacc actcaatgtt cagcgaaaat
       61 gcggaccggt taacgcgaaa tgtgtacaag gtcattgatc actacaacgg aataatgcag
      121 gaatccaatt attgatggac gccacgttgg aatttgagat catcgggaaa taggtgggaa
      181 ggcgattggc tgaacgaacg tggatcgtgt gattggttat tcgttattag tgattggatt
      241 gttaaatcga agacgcacct ctgtgaacaa aaccatcccg aatcactaat caccaatcac
      301 taatcaccaa tcaccaatca ccacatcaat caacgaactc gcttcctaca ccatcctccc
      361 actccaacag cacgaatgaa tgagcagtag caaatgaaat gtcttcagtc ggttggcagc
      421 ttccggaaag gcgccttcaa taaggtaccc aggtcttcgt cccgttcaac cgggaagttg
      481 cgatccaacc cgtaccggat ctcggaaggc tggagtgccg tcagggtgcc cagcagccgg
      541 tcccagaccg acaaaaccgc tccgtaattg ctgtcggtgt agggctgctt ccagtggtga
      601 tgcactttgt gcatgttggg cgataccagc acatagccaa tggcgttatc cagccagtcg
      661 ggcaagcgga tgttcgcatg cgtaaaggcg gtggcgaaaa ccaggacggt ctgatagaac
      721 atgacggcaa cgatcggcgc gccgctgatc catacgccgg cgaagaagaa caagccccga
      781 aacaggctct ccgccggatg gtgccggagc ccggtggtgg cgtccacgtt cgtatcggca
      841 tggtggatga tgtggaaccg ccagaggaag ggaaccttgt gttgcgtgat gtgcaccagc
      901 cagccgacga aaaagtcgag cgtcaggaaa ctcaccagca cggtgaggcc gatgcccgca
      961 tccagccagt acacgagtcc gaattgatgt tgcctgcacc agtcgctgat gaggatgatc
     1021 acgaacgcga gcaacccgtg tatgatcagg tgcaggaagg tgaagaacag attgacgccg
     1081 gcatgccgga cgcgcgtttt ccggaaacga agcggtaaca acgggatcgc cccttccagt
     1141 atccagaaaa acagcagccc gccgatcagg atgcccatgc gctgcagcgg atgggcttgc
     1201 agggtttcga aaaagtgaag gatccgatcc ataccgaaag gtacgcaatc gccggcctca
     1261 gcggatcagg ttcacgttgc cgctcgcttc ccgccactcg cccttcgggt cctgtgcgcg
     1321 gatcttgtac acgtaaacac cctccgggca caggctttcg ccgccctggt agttgccgtt
     1381 ccagccttgt tgcagcgaat cggaacggaa gatcaccttg ccccagcgat caaagaccag
     1441 caactcgaag gcatggacgc cgatgccata cgcgtagaac agctcgttct tgccgtcgcc
     1501 gttcggcgtg aaggtgttcg gtacgtacaa cgcgtagtct tcgttcacgc gcacgcgcgc
     1561 gtaggcggtg tcgcggcaac cgtgcggact caaggcgatc aaccggatca cgtaggtgcc
     1621 ggtgtcggca taggtgtaaa cgggatcctg gtctgacgaa attcccgccg catcaccaaa
     1681 gtcccatgcc caggcgctcg cgtcgctgct ggcgtccgtg agccggatca ccggtgtcag
     1741 gatcgtggtc tcctcggtgc tgacgttgaa gtccgctacc ggcagcggat ctacggtcac
     1801 cgcttccgct tcaaagtagg aactgcatcc gtgcggggta gtcacctcca accgaacgga
     1861 gtaggtaccc gcgtccggga aggacagctc cggatgacga ctggtatcgg tgatgccgtt
     1921 gtacgtccat tgatagtgcg agccggcttc cgcggtcgac agatcgaaaa agcggacctg
     1981 caagggcgcg caaccggaga ccggattcgg caggaagttg acgacgggtg tcggaaatac
     2041 ttccaggaat atgctggcgg aaatgctgca acccagctag gggccggtga cggtgta
//
LOCUS       DAOTON010000533         2096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41217_length_2096_cov_3.601176, whole genome shotgun sequence.
ACCESSION   DAOTON010000533 DAOTON010000000
VERSION     DAOTON010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41217_length_2096_cov_3.601176"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="P5292_10060"
     CDS             complement(<1..250)
                     /locus_tag="P5292_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRS39512.1"
                     /translation="MFVFDKIKPLQTVIHEWKSTGLRIGFVPTMGALHPGHISLVAEA
                     HRQCDRVVCSIFVNPTQFNNPDDLKRYPRMPEADRSMLE"
     gene            443..1279
                     /locus_tag="P5292_10065"
     CDS             443..1279
                     /locus_tag="P5292_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRS39513.1"
                     /translation="MKKAKVLFVSQEITPFLEVTEVSNIARRLPQGVQERGKEIRTFM
                     PRFGVINERRNQLHEVIRLSGMNLIIDESDHPLIIKVASIQSARMQVYFIDNEEYFQR
                     KNVFRDAKKKFHKDNEDRMVFFCRGVLETVKKLGWAPDVIHCHGWMTSLIPFFVKTGY
                     KDDPMFRNSRIVYSSYLQDMEENFTPNLVKKLKMDGVTPDDLKLYKEPSMANVHLAAM
                     KLSDGIVRGCDTKHSALDTYLKKTNKPVLQYSPFDDDGNFVGACSDFYDELLENEPAL
                     VD"
     gene            1281..>2096
                     /locus_tag="P5292_10070"
     CDS             1281..>2096
                     /locus_tag="P5292_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HRS39514.1"
                     /translation="MLANSAGRFLAGLFGPGANPITDQPQKNISSASVWRKCFFPALL
                     AFAFLSGCKDSEELGLSVLPESDPLSTAYSDSATIDCRVLTEDSVRGDELSASLLGAY
                     GDPVFGKTKASIYAEVLLQGSPTFVNLSTVDSLVLQLFYSGYYADTTTAQTVTVYRLK
                     ENINPGSTYYSFKDFAREDQPIGTRAYVPQPYTRIVVDSDTVSPQLRIPLDLALANEI
                     VSLSGQPALSGNSSWRNYFKGIYIESEGNTAAGKGCISTIDVFNSRLNLYYHDT"
BASE COUNT          506 a          582 c          515 g          493 t
ORIGIN      
        1 gctccaacat ggatcggtcg gcctccggca tgcgagggta ccgcttcagg tcgtcggggt
       61 tattgaactg ggtcgggttg acgaagatgc tgcagacgac ccgatcgcac tgccgatgcg
      121 cttccgccac cagggaaata tggccggggt gcaaggcacc catggtgggc acgaaaccga
      181 tccgaagacc agtcgacttc cactcatgaa tgacggtctg aagtggtttt atcttatcaa
      241 atacgaacat ttgatcgtaa tcaagcgctt tgggcgacca tcttgtcgtt cggacttacc
      301 aatctgatga tcaaactatt aaacttaaat aagtcctttt tttcttaaaa atcgattgtt
      361 tttcggtcag ggggcttgcg catattgaag cgtaccctta ttatgtgtat ctttgcagct
      421 gtttaaccat tcatttccta taatgaagaa ggcgaaagta ctattcgttt cccaggaaat
      481 tacccctttc ctggaagtca cagaagtatc caacatcgcc cgccggctgc cgcaaggagt
      541 ccaggagcgc ggaaaagaga tccggacgtt catgccccgt tttggtgtga tcaacgagcg
      601 tcggaaccaa ctccacgaag tcatccgcct ctccggcatg aacctgatca tcgacgaatc
      661 ggatcatccg ctcatcatca aggtagcctc gatccagagt gcccggatgc aggtttattt
      721 catcgacaac gaagaatatt tccagcggaa gaacgttttc cgtgacgcga agaagaagtt
      781 ccacaaggat aacgaagacc ggatggtctt tttctgtcga ggcgtactgg aaaccgtgaa
      841 aaaacttggc tgggcaccgg atgtgatcca ttgtcatggc tggatgacca gcctgatccc
      901 gttcttcgta aagaccggtt ataaggacga cccgatgttc cgcaattcgc gcatcgtgta
      961 ttcttcctac ctccaggaca tggaagaaaa tttcacgccg aacctggtga agaaactcaa
     1021 aatggacggt gttacgccgg acgacctgaa gctgtataaa gagccctcca tggccaatgt
     1081 gcacctggcc gcgatgaagc tcagcgacgg gatcgtacgc ggttgcgaca ccaaacattc
     1141 cgcactcgat acttacctca agaaaaccaa caagcccgtt ctccagtatt caccattcga
     1201 cgacgacggc aacttcgtcg gcgcctgttc ggacttttat gacgaactgc tggaaaacga
     1261 gccggctctc gtagactgat atgctcgcaa actccgccgg ccgtttcctg gccggtcttt
     1321 tcggccccgg ggccaaccca attaccgatc aacctcaaaa gaacatttcc tccgcatccg
     1381 tgtggaggaa atgttttttt ccggccctgc ttgcgttcgc ctttctgagc ggatgtaaag
     1441 actcggaaga actcggattg tcggtattgc ccgaaagcga cccgctgtca accgcttatt
     1501 ccgattcggc aacgatcgac tgcagggtcc tgacggaaga ttccgtccgg ggcgacgaac
     1561 tttcggcttc cctcctgggc gcctatggcg atccggtgtt cggcaagacc aaagcgtcca
     1621 tctacgccga ggtcttgtta cagggtagtc cgacgtttgt caacctgagt accgtagact
     1681 cgctcgtcct gcaactcttt tattcgggtt attatgccga taccaccacg gcacaaaccg
     1741 taacggtcta ccggcttaag gagaatatca atcccggttc gacctattac agtttcaagg
     1801 acttcgcccg cgaagaccaa ccgatcggaa cacgcgcgta cgtcccccaa ccttatacca
     1861 ggatcgtcgt tgacagcgac acggtatctc cgcagttgcg cattccgctc gaccttgcgc
     1921 tggccaatga gatcgtttcg ctaagcggtc aacccgcact ttccggaaat tcaagctggc
     1981 ggaactattt caaaggcata tacattgaat ctgaaggcaa taccgcagcc ggcaagggct
     2041 gcatttcgac catcgacgtc ttcaatagcc ggttgaacct ctattaccac gatacc
//
LOCUS       DAOTON010000534         2096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41223_length_2096_cov_3.267026, whole genome shotgun sequence.
ACCESSION   DAOTON010000534 DAOTON010000000
VERSION     DAOTON010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41223_length_2096_cov_3.267026"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="P5292_10075"
     CDS             complement(<1..428)
                     /locus_tag="P5292_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39515.1"
                     /translation="MKKLLLAVALLTAAFSTAIAQSEEHKHCYTTEKLQEAIRRDPTV
                     AQRMAQLEDFTRTWIADKAQQRSQGGIYIIPVVFHVLHNYGQENISDAQIIDAVRVMN
                     EDFRLLNADTNLIIPQFKQIAADCEIEFRLATIDPNGNCT"
     gene            complement(651..1808)
                     /locus_tag="P5292_10080"
     CDS             complement(651..1808)
                     /locus_tag="P5292_10080"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HRS39516.1"
                     /translation="MSTTNDHGFGTRAIHAGQSPDPSTGAIMTPIYQTSTYVQELPGK
                     HKGYAYARGKNPTRVALEQCIASLENAKHGLCFSSGQGAEDAIIKLLRPGDEVIATDD
                     LYGGSYRMFTKVFAHYGIVFHFVNMHDVNEINKHINDKTRMLWVETPTNPLMKIIDIR
                     GVAAIAQAKKLMLVVDNTFASPYLQNPLDLGATIVVHSATKYLGGHSDVVMGATCTND
                     DKIQEELAFIANSCGAVPGPMDSFLVLRGLKTLHVRVQRHCENGKAVAEFLKSHPKID
                     KLHWPGFPDHPNHAIAKSQMRGFGGMISFTLKGDSLEEAFRMASSTKVFSLAESLGGV
                     ESLIGHPATMTHASIPKEMREKSGVLDSLLRLSVGIEDANDLIADLRQALG"
BASE COUNT          423 a          572 c          599 g          502 t
ORIGIN      
        1 ttcgtgcagt tgccgttcgg gtcgatggtg gccagacgga actcgatttc acagtcggca
       61 gcgatctgct tgaactgcgg gatgatcagg ttcgtatcgg cattcagcaa tcggaaatct
      121 tcgttcatca cgcgcacggc gtcgatgatc tgcgcgtcgc tgatgttctc ctgcccgtag
      181 ttgtgcagga cgtgaaagac caccggaatg atgtagatac caccctggct gcgctgttgc
      241 gccttgtcgg cgatccaagt ccgggtgaaa tcttccaact gcgccatgcg ttgagcgacg
      301 gttggatctc gtcgaatcgc ttcctgtagt ttttcggtcg tgtagcagtg cttgtgctct
      361 tccgactggg caatcgcggt actgaacgct gctgtcagca gcgcgacggc aagtagtagt
      421 tttttcatgg tgatggaact gctgtttctg ttgtttaggg ataacagcta aaaccgttca
      481 aagataacca attggcagta ttttcctgaa taccaacaca ctcttgacaa agcgctgaca
      541 ggacgacagg acgcaggtcc ttgacgaaaa accctcctga aatgatgaaa aacgggccga
      601 attcatgaag aaatcggccc gtttaacgga ctgtcagttc gtgtcagtgc tcagcccaat
      661 gcctgacgaa ggtcggcgat caggtcgttc gcgtcttcaa tgccgacgct cagtcgcagc
      721 agtgagtcga gtacgcccga cttttcccgc atttcctttg gaatggaggc gtgggtcatc
      781 gtggccggat gaccgatcag ggattcaacg ccgccgaggg attcggccag cgagaaaacc
      841 ttggtactcg acgccatccg gaacgcctct tcgagcgagt cgcctttgag ggtgaacgag
      901 atcatgccgc cgaagccacg catctgcgac ttcgcgatgg cgtggttcgg atggtcggga
      961 aagcccggcc agtgcagttt gtcgatcttc ggatgcgact tcaggaactc cgcaaccgcc
     1021 ttgccgtttt cgcaatggcg ttgcacccgt acgtgcagcg tcttgagtcc gcgcagcaca
     1081 aggaagctgt ccatcggccc cggcaccgct ccgcaggagt tggcgatgaa agcaagttct
     1141 tcctggatct tgtcgtcgtt cgtacaggta gcgcccatca cgacgtcgga atggccaccg
     1201 aggtatttgg ttgcagaatg cacgacgatg gtagcaccca ggtccagcgg attctgtaga
     1261 taaggagacg cgaaggtgtt gtcgaccacc agcatcagct tcttcgcctg cgcgatggct
     1321 gccaccccgc ggatgtcgat gatcttcatc agcggattgg tcggggtctc gacccacagc
     1381 atccgcgtct tgtcgttgat gtgcttgttg atctcgttca catcgtgcat gttcacgaaa
     1441 tggaacacga tgccgtagtg cgcgaaaacc ttggtgaaca tgcggtacga accgccgtac
     1501 agatcgtccg tggcgatcac ttcatcgccg ggtcgcagca acttgatgat ggcatcttcc
     1561 gcgccctgtc cgctcgagaa gcataatccg tgcttcgcat tctccaggga agcgatgcac
     1621 tgctccagcg ccacacgcgt cggattcttt ccgcgggcat aggcatagcc tttatgcttt
     1681 cccggtaatt cctgtacgta cgtggatgtt tggtaaatgg gcgtcatgat cgccccggta
     1741 ctcgggtccg gcgactgccc ggcatggatc gcccgcgtcc cgaaaccgtg atcgttggtg
     1801 gttgacatgg ggcaaaggta ggaaatcggg aacgaggtgg agggttccga gttccgggtt
     1861 tcgtgtttcg ggttgttgtt ggttattggt aattagtaat tggtaattag taattagtta
     1921 gttgtccgta gagcttctcc ctcggtctcg gtcacgctcc tcgtccctgc cttttaaaga
     1981 ccggaaactc gaaactcgaa actcgaaact cgagacccgg aacccggaac ccgaaaccct
     2041 cctccctcgg tctcggtcac gctcctcgtc cctgcctttt aaagaccgga aactcg
//
LOCUS       DAOTON010000535         2093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41312_length_2093_cov_2.814033, whole genome shotgun sequence.
ACCESSION   DAOTON010000535 DAOTON010000000
VERSION     DAOTON010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41312_length_2093_cov_2.814033"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..131)
                     /locus_tag="P5292_10085"
     tRNA            complement(59..131)
                     /locus_tag="P5292_10085"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96..98),aa:Thr,seq:tgt)
     gene            complement(823..1818)
                     /gene="gap"
                     /locus_tag="P5292_10090"
     CDS             complement(823..1818)
                     /gene="gap"
                     /locus_tag="P5292_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRS39517.1"
                     /translation="MIKIAINGFGRIGRMTTRVLLNMTGVEIVAINDLTDNATLAHLF
                     KYDSIHRRFNGTVESDENHLILNGRKIAALAEKDPSKLPWKSMGVDVVLECTGKFTDR
                     AGGEMHLAAGARKVLLSAPSKGKESVKTIVLGVNDGDLQPDDAIISNASCTTNNAAPM
                     LKILDDHFGIESAFVTTVHSYTGDQNIHDAPHKDLRRARAAAVSIIPTTTGAAKALGD
                     VLPHLNGKLGGAGVRVPAPDGSLTDITCILRKQTTVEEINGLFKSKAEGELKGILEYT
                     EDPIVSIDIVGNPNSCIFDAQLTTVLGSMVKVVGWYDNEIGYSNRLAELARRVGV"
     gene            complement(1847..>2093)
                     /locus_tag="P5292_10095"
     CDS             complement(1847..>2093)
                     /locus_tag="P5292_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRS39518.1"
                     /translation="LGLGESREEVVESMMDLRTSGVSILTLGQYLQPTREHLPVTEFI
                     HPDTFASLKEEGLRMGFRYVESGPLVRSSYHAEKHIL"
BASE COUNT          507 a          557 c          492 g          537 t
ORIGIN      
        1 ctgccccatt tggccgctct ggaatctccc caactttggt actaattatc tattcgcgag
       61 ccggtgaagg gactcgaacc cccgacctac tgattacaaa tcagtagctc taccaactga
      121 gctacaccgg cgaaattgcg cgattttcaa agaacacttc cccctcgaaa aagggactgc
      181 aaaggtagaa atcgcaattt aaatttcaaa ttcgactgaa aatattttag ttaggccgct
      241 tgaacagttg ccggaataca aaaaagacgc cccggctttg gcagccaagg cgtctttcgg
      301 gatgttaaat acctgatttt caggcgataa ctttcgccaa ggggtaacgc ttcttccaca
      361 ttttgaaggc ggccatacta tgtaccacca tggtggtttt atggtcgagt tgaaccttga
      421 ttcgcggtcc agtcgtcgtt acaggggcct tgattacttt tcccttgctt ttcgagcgat
      481 tcatcacggt tagggaatta ggattgatta ttcgtttaac aattaacaac cgaaagatgt
      541 tctttagctg aagttcaaga atcatgcctt aaaaaaggct gtccgaatca cttaaaaaga
      601 accttcgagg ccaaataaac gcctatttcc tctaacctat tggttaattt ttattaaagt
      661 ttcgcgccag actgtcagtg cccaattttt tattaaaaat caagaataat agcttcgaac
      721 ggtcattttg tcgctaaaat cccgcgcgta acttggtaaa tggaatccgg tggtatcggc
      781 gacgattgcg aatcagggtc ctgatggacg attatccgac aatcagaccc ctacccgcct
      841 tgccagttcg gctaaacggt tgctgtagcc gatctcatta tcataccagc ccaccacttt
      901 taccatactc ccgagtacgg tggtcagttg cgcatcaaaa atgcaactgt tcgggttgcc
      961 tactatatca atcgacacga tcgggtcttc cgtgtattcc aggatacctt tcagttcgcc
     1021 ttccgccttc gacttgaaca ggccgttgat ctcttcaacg gtggtctgct ttcggagaat
     1081 gcaggtgatg tcggtaagtg aaccatccgg cgcgggcacc cgaactccgg caccacccag
     1141 ctttccattc agatgcggca acacatcacc gagcgctttc gccgctccgg tcgtagttgg
     1201 aatgatacta accgctgccg cccttgcccg cctcagatcc ttgtggggag cgtcgtggat
     1261 gttctggtcg cccgtgtaac tatgcaccgt ggtcacgaat gcggattcaa tgccgaaatg
     1321 atcgtcgaga atcttgagca tgggagccgc gttgttcgtc gtacagcttg cattggaaat
     1381 gatcgcgtca tccggctgta aatctccgtc attcaccccc aacacgatcg tcttgaccga
     1441 ctcttttcct ttggacggag cagacagtaa caccttgcgt gctccggcgg ccaggtgcat
     1501 ttctccaccc gcacgatcgg tgaacttgcc ggtacattcc agcacgacat ccacacccat
     1561 cgacttccag ggcaacttgg aaggatcctt ctcggccagc gcggcgatct ttcggccatt
     1621 caggatcaaa tgattttcat ccgattcaac cgtaccattg aaacggcgat gaatggaatc
     1681 gtacttaaac agatgtgcaa gcgtagcgtt atcggtcagg tcgttgatgg cgacgatttc
     1741 aacgcctgtc atattcaaca acacgcgggt ggtcattcga ccaattcggc cgaagccgtt
     1801 gatagcaatc ttgatcattt gaaattgtat ccgtgaagcg ggagcattag agaatatgct
     1861 tctccgcatg ataacttgat cgaacgagcg gaccgctttc tacataccgg aaacccatcc
     1921 gaagaccctc ctctttcagg gaggcaaacg tgtcggggtg aatgaactcc gtcaccggaa
     1981 ggtgttcgcg tgtcggctga aggtattgcc ccagcgtcag gatggaaacg ccggaagtcc
     2041 gaaggtccat catggactcg actacctcct cgcggctttc cccgaggccg agc
//
LOCUS       DAOTON010000536         2092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41330_length_2092_cov_4.021109, whole genome shotgun sequence.
ACCESSION   DAOTON010000536 DAOTON010000000
VERSION     DAOTON010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41330_length_2092_cov_4.021109"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="P5292_10100"
     CDS             complement(<1..1280)
                     /locus_tag="P5292_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRS39519.1"
                     /translation="MPKRAYPFLNRDLSWLSFNARVLQEAEDPGVPLLERLRFLGIFS
                     NNRDEFFRVRVANVRRMARFGKKGREILGMDPVVLLERIQLITLAQQKKFDKLYIQLL
                     RELEKQKIFMVDERQLTNEEGAFVRKYFREQVHPQLAPIMIETAPKFPYLKDKSIYLA
                     IRLLRPANGKEKKTKFALLEVPTDVVSRFLVLPGSGDQHRIILLEDVIRYCLDDLFPN
                     FDYAVREAYTIKMTRDAELDIDSDVSKGWMEKIEKSVKQRKKGAPVRMAFDERIPTDL
                     LAFILRKIKLFKQEYLIPGGRYHNFKDFINFPRVGSKQLFYAPTTPVDHPDLRGQRSL
                     FDVIRQNDVLLSYPYHNFHPIVDLLREASIDPKVTSIHTTLYRAARNSSVVNALINAA
                     KNGKQVTVVVELQARFDEESNIFYANRLQEEGVRV"
     gene            complement(1296..1550)
                     /locus_tag="P5292_10105"
     CDS             complement(1296..1550)
                     /locus_tag="P5292_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39520.1"
                     /translation="MKADRREQRKKLALQLVLASEEILSKLHPGATLRVRKQLKSSAK
                     DIAKKFLREMKTAQAKVKPAAKPASKSKKKAAAPRARKKS"
     gene            complement(1757..>2092)
                     /locus_tag="P5292_10110"
     CDS             complement(1757..>2092)
                     /locus_tag="P5292_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39521.1"
                     /translation="YSTAFIICRRWDFPVGAIMLEPGLYETDTDAYADVIRQQDDRAQ
                     SLLLAGHNFSITHFLSFMLGKEVGEMPTCSVAVLDLELSSWNELKRGSGRLLELVLPK
                     ALPNNETGP"
BASE COUNT          491 a          574 c          514 g          513 t
ORIGIN      
        1 acccgaacgc cttcttcctg tagccgattg gcatagaaga tattcgattc ttcgtcgaag
       61 cgcgcctgca actcgaccac caccgtgact tgtttcccat tctttgcggc attgatgagg
      121 gcgttgacga cgctgctgtt tcgtgcggcg cggtacagcg tcgtatggat acttgtcacc
      181 ttgggatcga ttgacgcttc ccgcaagagg tcgacgatcg ggtggaaatt gtgatacgga
      241 taactcagca acacatcatt ttgccgtatg acatcgaaca ggctgcgctg tccgcgcaga
      301 tcgggatgat ccaccggggt tgtcggagca taaaacaact gcttcgaccc aacccgcggg
      361 aaattgataa agtccttgaa gttgtgataa cggccgccgg gaatcaggta ctcctgcttg
      421 aagagtttga tctttcggag gatgaaggcc agcagatccg tcgggatccg ttcgtcgaac
      481 gccattcgca ccggcgcgcc tttcttgcgc tgcttgacac tcttctcgat cttctccatc
      541 caacccttcg acacatcgct gtcgatgtcg agctccgcgt cacgcgtcat cttgatcgta
      601 taggcttcgc gcaccgcata atcgaaattc ggaaagaggt cgtccaggca ataccggatc
      661 acatcttcga gtagtatgat ccggtgctga tccccggaac ccggcagtac caggaagcgc
      721 gacaccacat cggtgggtac ttccaggaga gcgaacttcg tcttcttctc cttcccgttc
      781 gccggtcgca acaggcgaat ggcgagatag atcgatttgt ccttgagata cggaaacttc
      841 ggggccgtct cgatcatgat cggcgccaac tgcgggtgta cctgctcgcg aaaatatttt
      901 cggacaaagg caccctcctc gttcgtcaat tgacgctcat cgaccatgaa gatcttttgc
      961 ttctccaatt cacgcaacaa ctggatgtag agcttgtcga acttcttttg ctgagcaagg
     1021 gtgatcaact ggatgcgctc cagcaaaacg accggatcca tccccagaat ctcgcgacct
     1081 ttttttccaa aacgagccat gcgccgtacg ttggcaacac gcactcgaaa aaattcatcc
     1141 cggttgttgg aaaagatccc cagaaaccgg aggcgctcca gcaacggaac tcccggatct
     1201 tcggcttcct gcaacacgcg ggcattgaaa gacagccagc tcagatcccg gttgagaaat
     1261 gggtacgcgc gcttaggcat aaagaaattt aggaatcagg atttcttccg ggcccggggt
     1321 gccgcggctt tcttcttcga cttgcttgcc ggcttggcgg caggcttgac tttcgcttgc
     1381 gccgttttca tttcgcgaag gaattttttt gcaatgtcct ttgccgaact tttcagttgc
     1441 ttccgaacac gcaaggtcgc accggggtgc agcttgctca gtatctcttc cgaagccaat
     1501 accagttgca aagcgagctt tttacgctgc tcgcgtcgat ctgctttcat tttatctgtc
     1561 ataaaactcg tttgaatggg caatttccgt aatccgaata gccgacaacg cccatattct
     1621 gaaacttaac agtgaattaa cgctgcgtct accctttaag atacgccgac cgacttcccg
     1681 ctgtcaagta taccggtaaa aaggttggat gagttctgtc atcccgccaa taacatgttg
     1741 ataaacaagc aattgcctat gggcccgttt cgttgttggg caaggctttc gggagcacca
     1801 actccaataa tcgtccagac ccgcgcttta attcattcca ggaagaaagt tcaagatcca
     1861 agaccgctac ggaacaagta ggcatctccc cgacttcctt tccgagcatg aacgacagga
     1921 aatgtgtgat cgaaaaatta tgcccggcca ataacagcga ttgtgcacga tcatcctgct
     1981 gtcggattac atcagcatat gcatctgtgt cggtctcgta cagacctggt tcgagcatga
     2041 tcgcaccgac gggaaaatcc caacgacgac aaataatgaa tgcggtcgaa ta
//
LOCUS       DAOTON010000537         2092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41337_length_2092_cov_3.305351, whole genome shotgun sequence.
ACCESSION   DAOTON010000537 DAOTON010000000
VERSION     DAOTON010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41337_length_2092_cov_3.305351"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="P5292_10115"
     CDS             complement(<1..251)
                     /locus_tag="P5292_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39522.1"
                     /translation="MKRRDFLRTAIPAAVVPAVLNGLPLKAFASSPLAEAISATDNDH
                     VLVMIQLNGGNDGLNTIIPLDQYANLTRARQNILPLANR"
     gene            complement(256..1953)
                     /locus_tag="P5292_10120"
     CDS             complement(256..1953)
                     /locus_tag="P5292_10120"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRS39523.1"
                     /translation="MDRRSFLSLSFQDVLTEPGYGEGASLSASSLTPYSGQWTDKEVK
                     HLLRRTQFGAPKAEIAYFRSLGCSASVDTLLERINHPAYTPPAPPVNHYYNDAQDPLV
                     GPEQPWPGTADTEGPGNISIRRREGLKAWWVGLMINQPRSLREKMTLFWYNHFVIEMR
                     VVSQSTFCYNYLQMLRANALGNFKTLTREVTISAGMLNYLNGDSSTASAPNENYAREL
                     QELFTIGKDANGQSLYTEDDVVAAARVLTGWTNDLVNGVSANGYLSVFDPARHDTGSK
                     TFSSFYSNRVITGRAGASGAQEVDDLLDMIFARNDAALFICRKLYRFFVYYKIDATTE
                     TNVIQPLAAILRQNNYDISAALSTLFKSEHFFDMLSQGCVIKPPIDFVVGLCRDFNAD
                     LPATATVPSKYAFWSSIVDQAGRMQQDIGDPPSVAGWPMYYQAPMFHEMWINTDTLPR
                     RNKVSDEMAGNAQTTGGLGVNIDVVGYTATMNNPSDPVALIDEVLRLHYFFDASPSVI
                     NYLLNILLSGQSQTYYWSDAWDNYMNDPTNPTYYGTVKSRLQTFYRYIMDLSEYQLS"
BASE COUNT          437 a          549 c          641 g          465 t
ORIGIN      
        1 atgcggttcg cgaggggcag gatgttctgc cgggcccgcg tgaggttcgc atactgatcg
       61 agcggtatga tggtattcaa gccgtcgttg ccaccattga gctggatcat caccagcaca
      121 tggtcgttgt cggtggcgct gatcgcctct gccagtggag aactggcgaa tgccttcaac
      181 ggcaaaccgt tgagtacggc gggcacaacg gccgcgggga tggcggtacg aagaaaatct
      241 ctacgcttca tgctgtcagg aaagctggta ttcggaaagg tccatgatat aacggtagaa
      301 cgtctggaga cgcgacttga cagtcccgta gtacgtgggg ttcgtcgggt cgttcatgta
      361 gttgtcccag gcgtcggacc aatagtacgt ctggctttgt ccgctgagga ggatattcag
      421 gaggtagttg ataacggagg gtgatgcgtc gaagaagtag tgcaggcgca acacttcatc
      481 gatgagtgct accggatcgg aagggttatt catcgtggcg gtgtaaccga ccacatcgat
      541 gttgacgccc aagcctccgg tggtctgtgc attcccggcc atctcatcgc tgactttgtt
      601 ccgccgcggc aaggtgtccg tgttgatcca catctcatgg aacatcggtg cctggtagta
      661 catcggccag ccggccaccg agggcggatc accgatgtcc tgctgcatgc ggccggcctg
      721 gtcgacgatg ctgctccaga aggcgtactt cgaaggaacc gtcgccgtcg ccggaaggtc
      781 ggcattgaaa tcccggcaaa gtcctaccac gaaatcgatc ggcggcttga ttacgcaacc
      841 ctggctgagc atatcgaaaa agtgttcgct cttgaacagg gtcgacaaag cggcgctgat
      901 atcgtaattg ttttggcgca gaatggcggc cagcggctgg atcacattcg tttcggtcgt
      961 agcgtcgatc ttataataca cgaagaagcg atacagcttc cgacagatga acaaggcggc
     1021 atcgttgcgc gcgaagatca tgtcgagcag gtcgtcgact tcctgtgcgc cggaagcgcc
     1081 ggcacgtccg gtgatgacgc ggttgctgta gaatgagctg aaggtcttgc tgccggtatc
     1141 gtgccgggcg ggatcaaata ctgacaggta tccattcgct gagacaccat tcaccaggtc
     1201 attcgtccag ccggtcagta cacgcgccgc tgcgaccaca tcgtcttcgg tgtagagtga
     1261 ttgtccattg gcgtcttttc cgatggtgaa caattcctgc agttcgcgcg catagttctc
     1321 gttcggagcc gatgccgtgc tgctgtcgcc gttgaggtag ttcagcatgc cggcgctgat
     1381 ggtgacttcc cgcgtcagcg tcttgaagtt gcccaacgcg ttggcacgca gcatctgcag
     1441 gtagttgtaa cagaaggtgg actggctgac cacccgcatc tcaatgacga agtggttgta
     1501 ccagaacaag gtcatcttct cccgtaaact gcgcggctga ttgatcatca gtccgaccca
     1561 ccaggccttc aagccctcgc gtctgcggat gctgatgttg ccggggcctt ccgtgtccgc
     1621 ggtaccgggc cagggttgct ccggtccgac cagcggatcc tgtgcatcgt tgtagtaatg
     1681 gttgacagga ggcgcgggtg gcgtataggc aggatgattg atccgctcca gtaaggtgtc
     1741 gaccgaagcg gaacaaccca gtgaacggaa gtaggcgatc tccgctttcg gagcgccgaa
     1801 ttgtgtccgc cgcagcaggt gcttgacctc cttgtcggtc cattgaccgc tgtacggagt
     1861 caagctactt gcggacagcg acgcgccctc gccgtagccc ggctcggtca gcacgtcctg
     1921 aaatgaaagg gagaggaatg aacgacgatc catcttcacg atttcctgaa caacttttac
     1981 tgtagtgtcc agataacgat acaatgaacg gggccgaatt tgggagcgga atgaataaag
     2041 ccggttagct gaaacccgct attgacaggt ttttcaacag gtcgaaggct ga
//
LOCUS       DAOTON010000538         2079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41698_length_2079_cov_3.344368, whole genome shotgun sequence.
ACCESSION   DAOTON010000538 DAOTON010000000
VERSION     DAOTON010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41698_length_2079_cov_3.344368"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="P5292_10125"
     CDS             complement(<1..332)
                     /locus_tag="P5292_10125"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRS39524.1"
                     /translation="MEKRNDSFRWEDKVIEHKRFIKAPRSLVWEVWTKPEHLAKWYGP
                     EGYTLTTAQLKLQTGGSWNFVMHGFGKDYQNDIRYTRIEPEHTLCFRNGAIDNPYGFD
                     VQVTLEEQ"
     gene            complement(316..666)
                     /locus_tag="P5292_10130"
     CDS             complement(316..666)
                     /locus_tag="P5292_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRS39525.1"
                     /translation="MSSPRRDVFQAISDPTRRAILGLLALQSLSVNAVAENFDISRPA
                     VSKHVKVLEECGLVHVRQEGRERFCEARLDQLAEVNAWVNQYRRFWERKFDRLEAYLD
                     QLQQSKKKHRGKKK"
     gene            803..1369
                     /locus_tag="P5292_10135"
     CDS             803..1369
                     /locus_tag="P5292_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRS39526.1"
                     /translation="MVSKNELPWIEAGYELFAAQGPDGLRVETLARKVGISKSSFYHH
                     FADIDVFTERLLEWHLDRAVEIAGRARDCRTFDPDFLHLLAEYPGDLLFNRQLRVHRE
                     NLSYQLCFTRANAFVEDAILACWSAELGLAGKPELARSLFAVINDMFYQRVTRENLHY
                     TWMKQLLGEIKAILTDMIRGSGMGNELI"
     gene            complement(1402..>2079)
                     /locus_tag="P5292_10140"
     CDS             complement(1402..>2079)
                     /locus_tag="P5292_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39527.1"
                     /translation="AYAEVEYKSNIILYSQWYLRGPQNNDGNTYTKCPLYHSLVWRVN
                     LNKWMEVLGEYSFIPVNILSQEYIKIEYERKDYWLRQGLEFVDNRIKLGIGVVLLNRA
                     ASGRIVNRFGLGGSFNSIINERYSIRVTPEVTGFPAGTSDNGPPLSGSLKRFYPQGYC
                     WIVSNEILWKKPLKGRIDLLLGCKYEYALYDDKDKFPAYLNNFKAVEIVSNKLISISL
                     GVSYRFK"
BASE COUNT          501 a          550 c          468 g          560 t
ORIGIN      
        1 tcctgctctt ccagggttac ctgcacgtca aagccgtagg gattgtcgat agcgccgttg
       61 cggaaacaca gcgtatgctc gggctcgatc cgggtgtagc ggatgtcgtt ctgatagtcc
      121 ttcccgaatc cgtgcatcac gaaattccag ctgcctcccg tttgcaactt cagctgcgcg
      181 gtggtcaggg tgtatccttc cggaccgtac cacttggcca ggtgctccgg tttggtccac
      241 acttcccaga ccagggagcg cggcgccttg atgaagcgct tgtgctcgat taccttgtcc
      301 tcccaacgga aggaatcatt tcttttttcc acggtgtttc ttttttgatt gttgcaactg
      361 atcgaggtaa gcttccagtc ggtcgaattt ccgttcccag aaccggcggt actgatttac
      421 ccaggcgttt acctccgcga gctgatccag acgggcttcg caaaagcgct cacgtccctc
      481 ctgccggaca tgcaccaggc cacattcttc gagcaccttg acgtgtttcg atacggccgg
      541 tcggctgatg tcaaagttct cggcgaccgc gttgaccgag agcgattgga gcgccagcag
      601 gccgaggatc gccctgcggg tcgggtcgga aatcgcctgg aagacatcgc gtcgcgggga
      661 gctcattgtg taaccgtttg gttacaaata tacgcgtaac cctttggtta cacatattgt
      721 cgcttaactt taacacttga acaggtagaa tgcctgtttc tgccaaattt ttaaaattgt
      781 aaaaggatta tttttacccg gcatggtttc caagaacgaa ctgccctgga tcgaagcggg
      841 ctacgaattg ttcgccgcgc aaggaccgga cggcttgcgg gtggagactt tggcgcgcaa
      901 ggtgggcatc agcaagtctt cgttctacca ccacttcgcc gatattgatg tcttcaccga
      961 acggctgctg gaatggcacc tcgaccgcgc ggtggagatc gccggacgcg cacgcgactg
     1021 ccggacgttc gatcccgact ttttgcattt actggccgaa tatcccggcg acctgctttt
     1081 caaccggcag ttgcgcgtac accgggagaa cctttcctac cagctttgct tcacccgcgc
     1141 caatgcattc gtcgaagatg cgatcctcgc ctgctggtca gcagaactcg gcctggccgg
     1201 caaaccggaa ctggcgcgca gcctcttcgc cgtcatcaac gacatgtttt accaacgcgt
     1261 gacccgcgaa aacctgcact atacctggat gaaacaactc ctcggcgaga tcaaagcgat
     1321 cctcaccgat atgatccggg ggagtgggat ggggaatgag ttgatttgat gtttccaaga
     1381 taaaatatga agtcttcgct ttcatttaaa tcgataagaa acgcccaatg aaatagaaat
     1441 taatttattt gaaactattt caacagcttt aaagttattc aggtatgccg ggaatttatc
     1501 cttatcatca taaagtgcat actcatactt acaccctaat aaaagatcta ttcgtccttt
     1561 taatggcttt ttccaaagta tttcatttga aactatccag caataacctt gaggatagaa
     1621 tctttttagg ctgccagaca aaggaggtcc gttgtctgat gtgccggcgg gaaagcccgt
     1681 aacttcgggc gttacacgga tactatacct ttcattaata atggaattaa aactccctcc
     1741 caacccaaac cgattaacaa tcctgcccga tgcagcccga ttaagtagca caacaccaat
     1801 gcctaacttg attcgattat ccacaaattc tagcccttgt cttaaccaat agtcttttct
     1861 ttcatactca attttaatat actcctgact taatatatta acaggaataa aactatattc
     1921 ccccaatact tccatccatt tatttaaatt aactctccat actaatgagt gatataatgg
     1981 acacttagtg taggtgttcc catcgttatt ttgcggaccc cttaaatacc actggctata
     2041 taatattata ttgcttttat actcaacttc cgcatacgc
//
LOCUS       DAOTON010000539         2078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41724_length_2078_cov_3.632229, whole genome shotgun sequence.
ACCESSION   DAOTON010000539 DAOTON010000000
VERSION     DAOTON010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41724_length_2078_cov_3.632229"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="P5292_10145"
     CDS             complement(<1..587)
                     /locus_tag="P5292_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39528.1"
                     /translation="MADQLSGMAERAGFPTFTLPLPRQYSSTMQKIALQPVRAVVFLA
                     LLFGSLSASAQGLTISTPQLDFGTVLDTQPDSLPLSVTNTLTRSVIVTDIRFYTTYGA
                     PAFSTPYHWFTLAPGASNTIWVKFSPKHNIYHNSELVIENDGLRGFPHVDLVGQGRYS
                     NAYYDSTENKSEEALKTAIKSTTTNGSISLGYNIAR"
     gene            625..1965
                     /locus_tag="P5292_10150"
     CDS             625..1965
                     /locus_tag="P5292_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS39529.1"
                     /translation="MKKLLLSLSIAAFGLTASAQINMNIGDKVKRKVEDRVNRKVDQG
                     IDKGLDKTEESAKKEVKKDKDAKTNSEKSGNDAASPGGNSGSSGSGSTAAASGGLKSY
                     GKFDFVPGDKILVVEEFAQDAIGDFPAQWNTNSTGELVNVEGRDGKWLSLTKDGVFLP
                     EFITALPENFTLEFDLICNDDFSFYSTAFSVNLCRLAAQKDAFNIWKQYGQQQNRDGV
                     QVSFHPQDAGGGQGRTNYIAYDKGSELMKNETATMQFYGKGKRESHVSIWRQKTRLRV
                     YINEEKVWDLPRAFVTGTTYNSVCFTTGGFHQSQDRYLISNLKLAVGAPDTRNKLITE
                     GRFSTSGILFDSGSDRIKPESYGVLKDIATVLKENPAVKVKIIGHTDSDGEDAANLDL
                     SKRRALSVSKALTSEFSIEASRMTTDGKGEKEPIADNTKNEGKAMNRRVEFVKE"
BASE COUNT          546 a          492 c          626 g          414 t
ORIGIN      
        1 cgggcgatgt tgtagcccag cgaaatcgaa ccgttggtag tggttgactt gatagccgtc
       61 ttaagcgctt cctctgattt gttctcggtg gaatcgtagt aggcattgga gtagcggccc
      121 tgtccgacca ggtcaacgtg ggggaaacca cgtaatccgt cgttctcgat caccagctct
      181 gagttgtggt agatgttgtg cttcggggaa aacttcaccc agatggtatt ggaggcaccg
      241 ggggcaagcg tgaaccaatg gtaaggtgtg gaaaacgccg gcgctccgta ggtagtatag
      301 aaccggatat cggtgacgat gacggaacgc gtcagtgtat tggtcacgct gagcggcagg
      361 ctgtcgggtt gggtatcgag tacggtgccg aagtcaagtt gcggggtgga aatggtaagt
      421 ccctgtgcgg aagcggaaag cgatccgaag agcagggcca agaatactac tgcccgaacg
      481 ggctgaaggg cgattttttg catggtagag gaatactgcc ggggcagcgg gagggtaaag
      541 gtagggaatc cggctctttc cgccattccg gagagttgat cggccatcgt ggcatttcgt
      601 aaattcgtac tcccaatcaa cgacatgaaa aagcttctgc tttccctttc gatcgcggcc
      661 ttcgggctaa ccgcttcggc gcagattaac atgaatatcg gcgacaaagt caaacgtaaa
      721 gtcgaagacc gggtcaaccg caaggtggat cagggcatcg acaaggggct tgacaagacc
      781 gaagaaagcg ccaagaaaga agtcaaaaaa gacaaggacg ctaaaacgaa ttccgagaag
      841 agcgggaatg atgcagcttc ccctggcggc aatagcggga gcagtggatc gggtagtact
      901 gcggctgctt cgggcggttt gaagagttac ggaaaattcg attttgtacc gggcgacaag
      961 atcctggtgg tagaagaatt cgcgcaggac gcgattggcg acttcccggc acagtggaac
     1021 accaattcga cgggagaact ggtgaatgtc gaaggtcgtg atggcaaatg gttgtcgctc
     1081 accaaggacg gcgtattcct gccggagttc atcaccgcct taccggagaa ctttaccctg
     1141 gagttcgacc tgatctgcaa cgacgacttc agcttctact ccactgcctt ctccgtcaat
     1201 ctctgtcggc ttgccgcgca gaaggacgct ttcaacatct ggaaacaata tggccaacag
     1261 caaaaccgtg acggcgttca ggtcagtttc catccgcagg atgccggtgg cggtcagggc
     1321 cgcacgaatt acatcgcgta cgacaaaggc agcgagctga tgaagaacga gaccgccacg
     1381 atgcaattct acggtaaagg gaagcgcgaa tcgcatgttt cgatctggcg gcaaaagacg
     1441 cgcttgcggg tgtatatcaa tgaagaaaaa gtctgggacc tgccgcgcgc gtttgtgacc
     1501 ggcaccacct acaacagcgt ttgtttcacc accggcgggt ttcaccagtc gcaggaccgt
     1561 tacctgatca gcaacctcaa actggccgtt ggcgcaccgg atacccggaa caagctgatc
     1621 accgaaggcc ggttctccac ctcgggcatc ctcttcgatt cgggcagcga ccgcatcaag
     1681 cctgagtcgt acggtgtatt gaaagacatt gccactgtgc tgaaagaaaa cccggcggtg
     1741 aaggtgaaga tcatcggtca tactgacagc gacggagaag acgccgccaa cctggacctc
     1801 tccaaacgac gtgcactttc cgtcagcaag gcgcttacca gcgaattctc catcgaggct
     1861 tctcggatga cgaccgacgg gaaaggcgaa aaagaaccga tcgccgacaa taccaaaaac
     1921 gaagggaaag cgatgaaccg gcgggtggag tttgtgaagg agtgaatagt gaatagtgaa
     1981 tagtgaatag tgaacagtga acagtgaaca gtgaatagtg aatagtgaat agtgaatagt
     2041 gaatagtgaa tagcgaatag cgaatagtga agagtgaa
//
LOCUS       DAOTON010000540         2077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41750_length_2077_cov_3.178536, whole genome shotgun sequence.
ACCESSION   DAOTON010000540 DAOTON010000000
VERSION     DAOTON010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41750_length_2077_cov_3.178536"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1862)
                     /locus_tag="P5292_10155"
     CDS             complement(<1..1862)
                     /locus_tag="P5292_10155"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HRS39530.1"
                     /translation="MDRIQKLLVANRGEIAIRVLRAASELGIRTVAVFTYEDRYSLHR
                     FKADEAYQIGQDSDPLKPYLDIEEILRVAKLNKVQAIHPGYGFLSENVQFARRCREEG
                     IIFVGPDPEVMEQLGDKVRSKEVARACGVPVIEDNKERLEDISMALKEARRIGYPIIF
                     KASAGGGGRGMRVIRQEEELEKAFTEARREAGNAFGDDTIFIEKYVDDPKHIEVQILG
                     DRHGNIVHLFERDCSVQRRFQKVVEIAPAPRLRQETREALYRHALAIATKVGYNNAGT
                     VEFLVDHAENVFFIEVNPRIQVEHTVTEEVTGIDLVRSQILIADGQPLHSSIIDIQGQ
                     DKIRCNGFAIQCRITTEDPQNEFKPDYGTIIAYRHAGGYGIRLDEGSSYQGVKVSPFF
                     DSMLVKVTAKGRTLKGTGKRLYRALSEFRIRGVKTNIEFLQNVITHPVFEAGEVTVRF
                     IEKHPDLFQVRAKQDQGTKLLRYLADVLVNGHPDVPKPDPSRVFQHAVVPSWDRSVKA
                     QSGTKQRLDELGPEKFAAWMKEQAGVQFTDTTLRDAHQSLLATRVRTQDMLRVAEAFA
                     QHHPQLFSMEVWGGATFDVALRFLHEDPWKRLQLLRSAIPNILLQMLIRGANAVG"
BASE COUNT          403 a          581 c          620 g          473 t
ORIGIN      
        1 ccgaccgcgt tggcgccgcg gatgagcatt tgcaggagaa tgttcggaat agcagaacgg
       61 agcagttgta aacgcttcca agggtcttcg tgcaagaagc ggagcgctac gtcgaaggtc
      121 gcaccgcccc agacttccat cgaaaagagc tgcggatggt gctgagcaaa ggcttcggct
      181 acgcgcagca tgtcctgcgt gcgcacccgg gtggcaagca gcgactggtg cgcgtcccgg
      241 agcgtggtgt cggtgaattg aacgccagct tgttctttca tccacgcggc gaatttctcc
      301 ggccccaatt cgtcgaggcg ttgcttggtt ccggattgcg cttttacact acgatcccag
      361 gatggaacca ccgcgtgttg gaacacacgg ctggggtccg gcttcggtac gtcgggatgg
      421 ccgttcacca gtacgtcggc caggtaacgc agcagcttgg tgccctggtc ctgtttggcg
      481 cgtacctgga aaaggtccgg gtgcttttcg atgaaacgta cggtcacttc cccggcttcg
      541 aacaccggat gcgtgatcac attctgcagg aactcgatgt tggtcttcac gccacggatg
      601 cggaattccg acagggcccg gtacaaacgc ttcccggtgc ctttcagtgt gcgtcccttg
      661 gccgtgactt tcacaagcat ggagtcgaag aagggcgaga ctttcacgcc ctggtaggaa
      721 ctgccttcgt cgagtcggat gccatagccg ccggcatgcc ggtaagcgat gatggtgccg
      781 tagtcgggct tgaattcgtt ctgcggatct tccgtggtga tgcggcactg gatggcgaac
      841 ccattgcagc ggatcttatc ctgtccctgg atatcgatga tggaggaatg caggggttgt
      901 ccgtccgcga tcaggatctg gctgcggacg agatcgatgc cggtcacttc ttcggtgact
      961 gtgtgctcga cctggatgcg cggattcact tcgatgaaaa agacattctc cgcgtggtcg
     1021 accaggaatt ccaccgtgcc ggcgttgttg taaccgacct tcgtcgcgat ggcgagtgca
     1081 tgacgataga gcgcttcgcg tgtttcctgc cgcagacgcg gagccggcgc gatctccacc
     1141 actttctgga atcgacgctg tacgctgcag tcgcgttcga acagatggac gatgttgccg
     1201 tgccggtcgc ccaggatctg cacttcgata tgcttcgggt cgtcgacgta tttttcgatg
     1261 aagatggtat cgtcgccgaa ggcattgcct gcttcgcggc gcgcttcagt gaaggctttt
     1321 tccaattcct cctcctgccg gatgacccgc attccgcgtc cgccgccgcc ggcactggcc
     1381 ttgaagatga tcggatagcc gatgcgccgc gcctctttca acgccatcga aatatcttcc
     1441 agtcgttcct tgttgtcttc gatgaccggt actccacagg cacgtgccac ttctttcgag
     1501 cggactttgt cgcccagttg ttccatgact tccggatcgg ggccgacgaa aatgatgcct
     1561 tcttcccggc aacgacgcgc gaactggacg ttctccgaga gaaaaccata gccgggatgg
     1621 atcgcctgta ccttgttcag cttcgcgaca cgcaggatct cttcgatatc gaggtagggc
     1681 ttcagcggat cgctgtcctg tccgatctga tacgcttcgt cggctttgaa acggtggagc
     1741 gagtaacggt cttcgtacgt gaataccgcg acggtgcgaa tgccgagttc ggaggctgca
     1801 cgcaatacac gaatagcgat ttcgccgcgg ttggcgacca gcaatttctg gatacggtcc
     1861 atggaagagg aaaatattgg ccatccaaag aaaagcaacc gcgcccctca cattccgagg
     1921 agagcagggg agttttaaca gaaggatgtt cggagttcgg gtccggcggc gaaatgtaat
     1981 ccagggttcc cgggttcaac ccttgatctc cgcaagcgca tccgcccagt tgtccttagg
     2041 caaatgacaa ctaccgtttt cacagacata atagagg
//
LOCUS       DAOTON010000541         2076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41790_length_2076_cov_2.377041, whole genome shotgun sequence.
ACCESSION   DAOTON010000541 DAOTON010000000
VERSION     DAOTON010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41790_length_2076_cov_2.377041"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..1611
                     /locus_tag="P5292_10160"
     CDS             319..1611
                     /locus_tag="P5292_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS39531.1"
                     /translation="MKQQTAIRSPFNLLVIVASLGYFVDIYDLILFNVVKKESLQALG
                     LGDGDYERNEIFLFNCQMIGMLLGGILWGVLGDRKGRLSVLFGSILLYSAANIANAFV
                     TTLDSYAVVRLLAGLGLAGELGAGITLVVETMSKETRGYGTMIIVTFGALGAVFASIV
                     GKEGAIMASWCNEHLGTTLVGWQMAYLVGGLLGLFLLFLRIGTIESGMYKKVKSESHV
                     RRGDFFALFRSRTLFFKYLSCIFIGLPIWYIIGILIALSGGITKELGIPDVVTGTAIM
                     YAYIGLSFGDLLSGLFSQFFRSRKKVVIGYILLSVVLVWLFLFPYSTSTDWFYLMCFL
                     LGSATGYWALFVTIASEQFGTNIRSTVTNTVPNFVRGAVVPITLLYKFLEPYFSTSPT
                     PKVYSALVVGLICSLLALIGILTLRDTFSKDLNYIEEC"
     gene            1676..2023
                     /locus_tag="P5292_10165"
     CDS             1676..2023
                     /locus_tag="P5292_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRS39532.1"
                     /translation="MVVGASEKPERYSNRAIRSLLLHGHEVLALAPRPGRVENVEFLT
                     GQPGLTDLDTVTLYLGEKAQETMMDYLLSLRPRRIIFNPGAENPELKKKAEALGIETE
                     EACTLVLLATDQY"
BASE COUNT          452 a          521 c          578 g          525 t
ORIGIN      
        1 tcgaaatcag gtgtgcggtc aaacgcaccg ggggttcgaa tccctccctc tccgcaacga
       61 gtactcagta caagaagcag ggcgagtgaa caattactgt tcactcgccc tgtttctgtt
      121 tctcacacta aataagtgca aagtgtgaga aacacggtgg gtggatagtt ttccacggtt
      181 cactcacctt gtttctcttt ctcacactaa aaaagtgcga agtgtgagaa acagtgtaag
      241 tgaacagtgg aaaaactacc cactcgcccg ctggaggccc attttctcct taggctggta
      301 tcctgtattt tgcattccat gaaacaacag accgctatcc gtagtccgtt caacctgctg
      361 gtcattgtcg cttccctggg gtacttcgtc gatatctacg acctgatcct cttcaatgtc
      421 gtcaagaagg aaagcctgca ggcgcttgga ttgggggatg gcgactacga gcgtaacgag
      481 atctttctct tcaattgcca gatgatcgga atgctgctgg gcggtattct atggggtgtg
      541 ttgggtgatc gaaaaggaag gctctcggta ttgttcgggt ccatcttatt gtattcggcg
      601 gctaacatag ccaatgcgtt cgtcaccacc ctcgacagct acgccgtagt gcgcctgctg
      661 gcaggactgg gactggcagg tgaactgggt gccggcatca ccctggtggt ggaaaccatg
      721 agcaaggaaa cccgcggcta tggcaccatg atcatcgtga cctttggtgc gttgggagca
      781 gtgtttgcgt ccatcgtggg aaaggagggg gcgatcatgg caagctggtg caacgaacac
      841 ctgggaacga ccctggtggg ttggcaaatg gcttacctcg ttggcggttt gctgggatta
      901 tttctgctct ttttacgtat cggaaccatc gagtcgggca tgtataagaa ggtgaaaagc
      961 gaatcgcacg tgcgacgagg cgacttcttc gccctcttcc gaagccggac tttgttcttc
     1021 aaatacctga gctgcatctt catcggcttg ccgatctggt acatcatcgg tatcctgatc
     1081 gccttgtcag gcggcatcac caaagaactc gggattccgg atgtggttac cggtacggcc
     1141 atcatgtatg cgtacatcgg actttccttc ggcgatctgc tcagcggatt gttcagccag
     1201 ttcttccgct ctcgtaagaa ggtggtgatc gggtatattt tgctttccgt cgtcctggtc
     1261 tggctgtttc tatttcctta ttccacctca acggattggt tctatctgat gtgcttcctg
     1321 ctcggttcgg caaccggata ctgggcgttg tttgtcacca tcgcttccga acagttcggc
     1381 acgaatatcc ggtcgacggt caccaatacg gtgcccaatt tcgtccgcgg tgcggtcgtg
     1441 ccgattaccc tcttgtacaa atttctggag ccctactttt caaccagtcc gacaccgaag
     1501 gtctacagtg ccttggtcgt gggactgatc tgttccctgc tggcactgat cgggatcctg
     1561 acgctgcgcg acaccttcag caaggacctg aactacatcg aggaatgctg acgaacagca
     1621 tagtgccggg cctggttgtt cggtatcttt gtgtatgacc gtttctaaaa gaaccttggt
     1681 cgtaggggct tccgaaaagc ctgaacgcta ttccaacagg gcgattcgtt cccttttact
     1741 gcatggccac gaagtattgg cccttgctcc acgaccggga agggtagaaa atgtagagtt
     1801 tcttaccgga caacctggct tgacagatct ggatacggtg acactctacc tgggtgaaaa
     1861 ggcgcaagaa actatgatgg actacctgct gtcccttcgt ccacgccgga tcatcttcaa
     1921 tcccggagcc gagaatcctg aactaaaaaa gaaagcggaa gcgctgggta tcgaaacgga
     1981 agaggcgtgt acgctggtct tgttggctac agaccagtat tgagggacga gggacgaggg
     2041 acgagggacg agggacgagg gacgagggac gaggga
//
LOCUS       DAOTON010000542         2073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41859_length_2073_cov_3.096630, whole genome shotgun sequence.
ACCESSION   DAOTON010000542 DAOTON010000000
VERSION     DAOTON010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41859_length_2073_cov_3.096630"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /gene="rocD"
                     /locus_tag="P5292_10170"
     CDS             complement(<1..868)
                     /gene="rocD"
                     /locus_tag="P5292_10170"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRS39533.1"
                     /translation="MKTTVLDKTRTELAIEKEDRYGAHNYHPLPVVLERGEGVYVWDV
                     EGKRYFDFLSAYSALNQGHGHPRIVKALVEQAQKLALTSRAFHNNLLGEYEEFITRFF
                     GFDKVLPMNSGAEGVETAMKLARRWAYEKKGIPENQAKIIVCEGNFHGRTISIISAST
                     DPDSYGRFGPFTPGFVKIPYNDAAALRKALEDPLVAAFLVEPIQGEAGVVVPDEGYLA
                     ACSAACREKQVLFIADEIQSGLARTGKMLACDHEDVRPDILILGKALSGGVYPVSAVL
                     ADDPIMLCIQPGQ"
     gene            986..>2073
                     /gene="rlmD"
                     /locus_tag="P5292_10175"
     CDS             986..>2073
                     /gene="rlmD"
                     /locus_tag="P5292_10175"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRS39534.1"
                     /translation="MTLPKPGDLLENLPLTDTSSDGRALARLENFVVFVEGGVPGDRA
                     DVHIYRKKKNFAEGRVERIVTPSPDRIDPVCSHFGSCGGCKWQHFSYAKQLESKQAYV
                     RDAFERIGKLDIPAQEPILGSADIFAYRNRLDFSCANKRWLTREELNNGASAVCNVIG
                     FHAPQRFDKVLDIDRCHLQDDRSNVIRKSVLHYATVNGFEFFDPLLQTGFLRNLTIRN
                     TSTDEWMVLVVFAYEDESKRIGLLQHLSDQFPYITSLQFTINGKRNDTFTGLDFVCFK
                     GKEFIEEEMEGLRFRISPKSFYQTNSQQAYRLYQVARDYAGLTGKENVYDLYTGTGTI
                     ALFVARQAARVAGIDYVEGAIGDARINAR"
BASE COUNT          494 a          562 c          543 g          474 t
ORIGIN      
        1 gctgccccgg ttggatacag agcatgatcg gatcgtcggc caggaccgcg ctgaccggat
       61 atacgcctcc ggacagtgcc tttccgagaa ttaaaatatc cggacgaaca tcttcgtgat
      121 cacaggccag catcttaccc gtacgggcca ggccggattg aatttcatcg gcgataaaca
      181 agacctgctt ttcccggcag gcagcggagc aggcagcgag ataaccttca tcgggcacca
      241 cgacaccagc ttctccctgg atcggctcta ccaggaaggc tgcaaccagc ggatcttcca
      301 gggctttacg cagggctgcc gcatcgttgt aagggatctt tacgaagccg ggcgtgaacg
      361 gcccaaatcg tccgtaagaa tccggatccg tagaagctga aatgatcgag atggtgcgac
      421 cgtggaagtt accttcacag acgatgatct tggcctggtt ttccggaatg cctttcttct
      481 cgtaagccca acgtctggcc agcttcatgg ccgtttcaac accttctgca ccggaattca
      541 tcggcaacac cttatcgaag ccgaaaaaac gggtgatgaa ctcctcgtat tctcccagca
      601 ggttgttgtg aaatgcccgg gaagtcaggg caagcttctg agcctgttcg accagggctt
      661 tgacgatgcg tggatgaccg tgtccctggt tgagggcgga ataagccgac agaaaatcaa
      721 agtatcgttt cccttcgacg tcccatacat aaacgccttc accgcgttct aaaaccaccg
      781 gaagcgggtg ataattgtga gcgccgtacc ggtcctcttt ttcaatcgcc agttcggtac
      841 gggtcttatc cagcacggtg gttttcatgg aattcggcat aaaatacaaa ggtaatgaat
      901 cggctgccgg gatgccaccc aagttccgcg aagtcgctcg ctttttgggc gagtgaaccg
      961 tttttccgtg ctattttcgc aatctatgac acttccgaaa cccggcgatc tgctggaaaa
     1021 cctcccgctg actgatacca gttccgatgg cagagccctg gcgcgactgg agaatttcgt
     1081 tgtcttcgtg gaaggcggag tacccggcga tcgggcggat gtacacattt accgaaagaa
     1141 gaaaaacttc gcggaaggtc gtgtggaaag aatcgtgacc ccttcacccg accgcatcga
     1201 tcctgtctgt agccatttcg gttcatgtgg cggctgtaaa tggcagcact tttcatatgc
     1261 gaagcaactt gaatcgaaac aggcgtacgt gcgcgacgca tttgaacgca tcggaaaatt
     1321 ggatattccc gctcaggagc ctatcctggg ttccgcggac atcttcgcgt accggaatcg
     1381 tctcgacttc agttgcgcga acaaacgctg gctgacccgg gaggagttga acaacggagc
     1441 aagcgctgtc tgcaatgtca tcggatttca tgcaccgcag cgctttgata aggtactcga
     1501 catcgatcgt tgtcatctgc aggacgatcg ttccaatgtc atccggaaat ccgtactcca
     1561 ctatgcgacc gtgaacggat tcgagttttt cgacccgctt ttgcagaccg gttttctccg
     1621 taacctgacc attcgcaaca catcgactga cgagtggatg gtgctggtgg ttttcgctta
     1681 cgaagacgag tcgaaacgga tcgggttatt gcagcatctg tcggaccaat tcccatacat
     1741 cacttcgctg caattcacaa tcaacggcaa acgcaacgac acctttaccg gactcgattt
     1801 cgtctgtttc aaaggaaaag aattcattga agaagaaatg gaaggattac ggttcaggat
     1861 ctcccccaaa tccttttacc aaacgaattc gcaacaagcc tatcggctct atcaggtggc
     1921 acgcgactat gcgggattga ccggaaaaga gaatgtctac gatctctata ccggcaccgg
     1981 caccatcgcc ctgtttgttg cacggcaggc tgcccgggtc gcaggtatcg attacgtcga
     2041 aggagcgatc ggagacgcgc gcatcaacgc acg
//
LOCUS       DAOTON010000543         2069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_41943_length_2069_cov_4.228898, whole genome shotgun sequence.
ACCESSION   DAOTON010000543 DAOTON010000000
VERSION     DAOTON010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41943_length_2069_cov_4.228898"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..1057
                     /locus_tag="P5292_10180"
     CDS             341..1057
                     /locus_tag="P5292_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39535.1"
                     /translation="MRQLNKNWITEHHIDFEYKKYLLLAYLQHVSGQFTEQRLYPHLA
                     DLIEHYRDLRQLKDNKNQLYQQFPERLNGARLEEFRLVYEKLIRDDNLMQELNSIIDF
                     SLPQMELALREGKKIYDFVEEHCRIFPVGLLPLNKDAGYIFLRAGDVSDTIVYEYQLS
                     IYESPHERFRGIQVGYIATYEKGLLNTYESIKSDLLRFHRNLPNPAAYVIESEFLLPF
                     DETFLPIAKRALVRALSTAA"
     gene            complement(1064..>2069)
                     /locus_tag="P5292_10185"
     CDS             complement(1064..>2069)
                     /locus_tag="P5292_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRS39536.1"
                     /translation="IAAAARGVAVYIQLDAYGSGKLDDTLLSDLRSKGIRIRKYGRLI
                     SRGKLHLGRRMHHKITVMDNHTALVGGINISDNYSGWNGKPPWLDYAVVVQGETARQL
                     NRICRKRWKDPDPQHELPSRESDRVPFEGLSANDRVPVRITQNDFLRRKQETAASYRQ
                     AIRQARNSLVFVGGYFLPGGLARRLLKKAARRGVAISLIVSAESDVGVVRNSRRYLYG
                     WLLRNGIRIFEYHPSNVHGKLLLKDNSFVSIGSYDLNNLSTYSNIELNLDIRDLEFSE
                     IVRSELEAVVAGSCHEVTRTQHGQEGIFRKFVDWASYQLVKTLFILSVWLANRDDRDI
                     "
BASE COUNT          448 a          577 c          540 g          504 t
ORIGIN      
        1 acattggctt tgagctcgtt gttgcccgtg ttcagttgct gattggagtc cttgagcttg
       61 gcgttctctt cctgacactg ctgttcgcgt gccttggaat cttccagttg tcgcgccgga
      121 acacaccccc aaaacatggc gattaccatc ggaattgtga ggatccacgt gatatttctt
      181 gcccttgacc tcattttcgt ttaaattagg atagttttat ccttccaaaa ttagtcgggc
      241 gatttcaatg cccgccgtag ataaacgttg tttatcaaca taccgaccga gaaacccggt
      301 tttacgggtg ttcgtagaac ctgtcataag ccatactcct atgcggcagt tgaataaaaa
      361 ctggatcacc gaacatcaca tcgacttcga atacaagaag tacctgttgt tggcgtacct
      421 gcagcatgtg tccggtcagt tcacggagca gcgcttgtat ccccacctgg ccgatctgat
      481 cgagcactac cgcgatctgc gacaattgaa agacaacaag aatcagctct accagcagtt
      541 ccccgagcgg ctcaacggcg cacggctgga ggaattccgg ctggtgtacg aaaagctgat
      601 ccgtgacgac aacctgatgc aggagttgaa cagcatcatc gatttttcgc ttccccagat
      661 ggagcttgca ctacgggaag gcaagaagat ctatgatttt gtcgaagaac attgccggat
      721 ctttccggta ggacttctgc cgttgaacaa agacgcgggt tatattttcc tgcgtgccgg
      781 cgatgtttcg gatacgatcg tttacgaata ccaattgagc atatacgaaa gtccacacga
      841 acgcttccgg ggtatccagg tcggttatat agcgacctat gaaaagggac ttttgaatac
      901 gtatgaatcg atcaagtcgg atctcctgcg tttccaccgg aacctgccca accctgcagc
      961 ctacgtgatc gagtcggagt tcctgctgcc gttcgacgaa accttcctgc cgatcgccaa
     1021 gcgtgccctg gtgcgtgcct tatcgaccgc tgcctgaggc ggatcagatg tcccggtcgt
     1081 cccggtttgc cagccatacg ctgaggataa acagcgtctt gaccagttgg tacgaggccc
     1141 aatcgacgaa cttcctgaaa atgccttcct gtccgtgctg ggtccgcgtc acttcatgac
     1201 acgatccggc aacgaccgct tccaattcac tccgcacgat ttccgagaat tccagatcgc
     1261 ggatatccag gttgagttcg atgttgctat aggtactcag gttgttcagg tcgtaggacc
     1321 cgatactgac aaagctattg tccttcaaca gcagtttccc atgcacattg gacggatgat
     1381 attcgaagat ccggatcccg ttgcgcaaca gccagccgta gaggtagcgt cgactgttcc
     1441 gcaccactcc cacatcggat tccgccgaga cgatcaggct gatggctact ccccggcgtg
     1501 ccgctttttt caagagtcgt cgggcaagcc cgccgggcag gaagtagccg ccgacgaaca
     1561 caaggctgtt ccgtgcttgt cggatcgcct gccggtacga agccgccgtt tcctgcttac
     1621 ggcgtaggaa gtcgttctgt gtgatccgga cgggaacgcg gtcgttggct gacaatccct
     1681 cgaacggcac tcgatccgac tcgcgggaag gaagttcatg ctgcggatcc gggtctttcc
     1741 agcgtttccg gcaaatgcga ttgagctggc gggcggtttc gccctgtacg accacggcat
     1801 aatcgagcca gggcggtttg ccgttccaac cgctgtaatt gtcggagata ttgatcccgc
     1861 ccacgagcgc ggtatggttg tccatgaccg tgatcttgtg atgcatcctg cgccccaggt
     1921 gcagctttcc cctggaaatg agccgaccgt atttccggat gcggatgcct ttacttcgaa
     1981 ggtccgacaa caaggtgtca tccagctttc ctgaaccgta tgcatccaac tggatgtaaa
     2041 cggcgacccc tcttgccgct gcagcgatc
//
LOCUS       DAOTON010000544         2067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_42002_length_2067_cov_4.100895, whole genome shotgun sequence.
ACCESSION   DAOTON010000544 DAOTON010000000
VERSION     DAOTON010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42002_length_2067_cov_4.100895"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..1564
                     /locus_tag="P5292_10190"
     CDS             287..1564
                     /locus_tag="P5292_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRS39537.1"
                     /translation="MSDWTIILLTLVFSAFAAGSEIAFLSSNKLRIELDRSQGSFPAR
                     IIWNFVKSPSRFIATMLVANNISLVIYSIVMSERLLTHEVLTDRLPKGLHSEGSLLLI
                     QTFLSTIVILISAEFIPKVLFRLNPNGILNILALPIQAVYYLLYPIVWVLLGISRFIM
                     RRVFRIDYVEERPIFGRIDLDLYIRDITSKNSQAEEMDTEIRIFQNALDFKDVKVRET
                     MVPRNEIVAVDVTESIEELRKLFVETKLSRILVYRESIDNIIGFVHHSELFRRPATIQ
                     EVLLPVSIVPEVMNANDLLKQFTAQHRSVAVVVDEFGGTAGMATFEDVMEEIFGEIQD
                     EHDVPEEIEKQLGEHEYIFSGRLEIDYLNKKYGLEIPEHEAYETLAGFIFHHHENIPE
                     QSEEIVVPPYVITALKVRNNRIDQVRLRVSHER"
     gene            1768..>2067
                     /locus_tag="P5292_10195"
     CDS             1768..>2067
                     /locus_tag="P5292_10195"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRS39538.1"
                     /translation="MAVIGRIRKRVGLVIAFVGISMLLFILGDLVTSNTGLMNRNSDV
                     VGEIGGNKIRYSEFEKRVETLTENYKLNTRQETVDQNTTDMLREQGASKRGAEFST"
BASE COUNT          505 a          566 c          482 g          514 t
ORIGIN      
        1 gaagggggga cgggagaata gtaattagta attagtaatt agtaattagt gattggtgat
       61 tggtgattgg tgattggtga ttggtgattt gaaaccggtg gtataggatg aagtgtaaga
      121 agcatctttc aactttcaac cttcaacttt caaccttcaa ctttcaacct tcaatttcca
      181 accttcaact tccaacccac cactcaaccc ctcctacctc aaacgcacat ctgttcactg
      241 tttactgttc actatttact gttcactatt cactaccttc gtccgcatgt ccgactggac
      301 gatcattctg ctaacactcg ttttttccgc ctttgccgcc ggtagcgaga tcgcatttct
      361 ttcttccaac aagttacgca tcgagctcga ccgcagccag ggaagttttc cggcccggat
      421 catctggaac ttcgtcaagt ctccttcccg cttcatcgcg accatgttgg tggccaataa
      481 catctcgctg gtcatttaca gtatcgtgat gtcggagcgg ctactcaccc acgaggtgct
      541 cacggaccgg ctgccgaagg gactgcattc ggaaggatcg ctgttgctga tccagacgtt
      601 cctcagtacc atcgtcattc tcatctcggc ggaattcata cccaaggtct tattccgttt
      661 gaatccgaat ggcatcctga atatccttgc cctgcccatc caggcggtgt actatctgct
      721 ctacccgatc gtttgggtat tgctgggcat ttcgcgtttc atcatgcgca gggttttccg
      781 tatcgattat gtggaggaac ggcccatttt cggccgcatc gaccttgatc tgtacatccg
      841 cgacatcacc agtaagaact cccaggcgga agaaatggat accgagatcc gcatctttca
      901 gaatgcgctt gatttcaaag acgtcaaagt ccgcgagacg atggtcccgc gcaatgagat
      961 cgttgccgtc gacgtgaccg aatccatcga agagttgcgg aagttatttg tcgagaccaa
     1021 actttcccgc attctcgtgt accgggaatc gattgacaac atcatcggat tcgttcacca
     1081 ttcggaactg tttcgcagac ccgcaaccat ccaggaagtc ctgttgccgg tcagcatcgt
     1141 accggaagtg atgaacgcga acgacctgct caaacaattc accgcccaac accgcagcgt
     1201 tgccgtcgtg gtcgacgaat tcggaggcac ggccggcatg gcgactttcg aagacgtgat
     1261 ggaagagatc ttcggcgaga tccaagacga acacgatgtt cccgaggaaa tcgaaaaaca
     1321 actcggcgaa cacgagtaca tcttttccgg ccgactggaa atcgactacc tcaacaagaa
     1381 gtacggcctg gaaatcccgg aacacgaagc ctacgagaca ctggccggat tcattttcca
     1441 ccaccacgag aacatcccgg agcagagcga agagatcgtg gtgccgcctt acgtcatcac
     1501 cgcgttgaaa gtgcgcaaca accgcatcga ccaggtgcgg ttacgggtca gccacgaacg
     1561 ctgataccgg caatgcagcc tctggcttga ttttcaagag ttcacgccct tttgtatatt
     1621 cgccgctcgt tttcgggaag cgttcagccg aattatattt aagtaattga ttttcaatta
     1681 cttatcatta ttccgtaacc ttcgctttcg cttcccgtcc aacgaaatct tatttaattg
     1741 ttcaaccctt aattaacacg acctgcaatg gccgtaatcg gaagaattcg gaaacgcgtt
     1801 ggcctcgtaa tcgccttcgt tggtatttcc atgttgctct ttatcctagg tgacctcgtc
     1861 acctcgaaca ccggactcat gaaccgtaac agcgatgtgg ttggtgagat cggcggaaac
     1921 aagatccgtt actcggagtt cgaaaaacgc gtggagacgc tcaccgagaa ctacaaactc
     1981 aacacccgtc aggaaactgt cgatcagaac acaaccgaca tgctccgcga gcagggcgcc
     2041 agcaagcggg gcgccgaatt cagtacg
//
LOCUS       DAOTON010000545         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_42052_length_2065_cov_3.855224, whole genome shotgun sequence.
ACCESSION   DAOTON010000545 DAOTON010000000
VERSION     DAOTON010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42052_length_2065_cov_3.855224"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="P5292_10200"
     CDS             complement(<1..1098)
                     /locus_tag="P5292_10200"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRS39539.1"
                     /translation="MEQFISRVKSSLAERLELAQSFGKIGEWEFDFLSGSAIWSKEVY
                     NFYEVDLDLPPVLFHESDEYYRPEEKTRLFSLIERLRLTRTAQQDVFQVHLPNGKVKW
                     HRSLLIPEVDVNGQLVHMHGLLQDITDEMMARTRLKEEQVFFQSVLDSIPADIAVYSE
                     DQRYLYINETAVADAEARKWIVNKNDFDYFTMRGLPLDRAYKRQAQFRSMLEGKEKKF
                     FEEHSVKNGKDIYKLRQFAPIWDKQTGKLRYVIGYGVDITDWKLSEQRLVSSGIAMEA
                     STDGIAILGADETYQYLNNAHISMFGYDAEEDLKGKTWRAIYPNPEVERIEKILLPLL
                     MQEGYWQGETVGKKKDGTLFHQEITLSALPNG"
     gene            complement(1568..>2065)
                     /locus_tag="P5292_10205"
     CDS             complement(1568..>2065)
                     /locus_tag="P5292_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39540.1"
                     /translation="QYFLFSFFLLALTPSTNAQSAFVTRLDLGLGSERKLLVEWLIRQ
                     GFETDTLSQPVTETMAQGISTVLGKELFGETYQNREGNIRVRLFTDQANVVQRLQVQM
                     ANPAPGEAYNLKSSFRTSGYSTGSEFTNQEDNTHSLLLEKEDRRFTLVESDDGSWLLL
                     NGQMR"
BASE COUNT          463 a          531 c          531 g          540 t
ORIGIN      
        1 gccgttggga agtgcggaca aggtgatctc ctggtgaaac agcgttccgt ccttcttttt
       61 gcccaccgtc tcgccctgcc aataaccctc ctgcatcaag agcgggagca ggatcttttc
      121 aatgcgttca acttcgggat tcggataaat ggctctccag gttttccctt tcaggtcttc
      181 ctcggcgtcg tatccgaaca tcgagatgtg tgcgttattg agatactgat aggtctcgtc
      241 agcacccaga atcgcgatac cgtcggtcga agcttccatc gcgatgccgg aactgaccag
      301 gcgttgttcg gacaatttcc agtcggtgat gtcgacgccg tatccgatga cgtaccgcaa
      361 cttgccggtt tgtttgtccc agataggagc gaactgtcga agcttataaa tgtccttccc
      421 gttcttaacg gaatgttcct cgaagaactt tttttccttt ccctccagca tggagcgaaa
      481 ctgcgcctgc cgtttatagg cccggtcgag tggcaggccg cgcatggtga agtagtcgaa
      541 gtcgttcttg ttaacgatcc actttcgcgc ctcggcgtca gccactgcgg tctcgttgat
      601 gtatagatag cgctggtctt cgctgtatac ggcgatatca gccggaatgc tgtccagcac
      661 cgattggaag aaaacctgtt cttctttcag tcgtgtccgg gccatcatct cgtcggtgat
      721 gtcctgcaac aagccgtgca tgtgcaccaa ttgtccgttc acatcgactt ccgggatcag
      781 gagcgaacga tgccatttga ccttgccgtt cggtaagtgg acctgaaata cgtcctgttg
      841 agcggtccgc gtaagccgca ggcgttcaat gagactgaag agtcttgttt tttcttccgg
      901 tcgataatat tcatcgcttt cgtggaagag taccggtggc agatccaagt cgacttcgta
      961 gaaattatac acctccttcg accagatcgc cgatccactc aggaaatcga actcccattc
     1021 tccgatttta ccgaaggatt gggcaagctc cagccgctcg gccagtgatg actttacgcg
     1081 ggaaatgaat tgttccatca ggccaattcc attattgtac aagtctgcag gatggcttct
     1141 gaaacggaat gagattgccg tatttattca gtcaccgttt tcgaaaagtc ggtcgcaaca
     1201 actttccgcc actttttact ccttagtaaa gataaggatt tgacggggta attcagtagt
     1261 tcggacccgt tgttcagctt tttaatgccg gcttccgctc tgccaaatgt gcctggaacg
     1321 ctgatcgaac ggaggatgca gatactttca gtcaggtatc aaccggctca ggcatgccgt
     1381 ttattaatct gaattgctat tgttgaatgg cgcaaaacgg acaaatggcg gcattgcggt
     1441 agcagtattc ggttacgtga caaagtgaac aaacagctca tgggatgata atcaacctga
     1501 acccaggttg catttccggc gagcgatgga acaattccgg tgcctgaaga tcagagacaa
     1561 tgacaactta gcgcatttgc ccatttaaca gcagccaact gccatcgtcc gattccacca
     1621 gggtaaagcg acgatcttcc ttttccagca ggagtgaatg ggtgttgtct tcctgattcg
     1681 taaactcaga gccggtggag tagcccgatg tccggaagga cgacttcaaa ttatacgctt
     1741 cgcccggagc aggattggcc atctgtacct gtagtcgttg tacgacattc gcctggtctg
     1801 tgaacaggcg cacccggata ttgccttccc ggttctggta ggtctccccg aaaagttcct
     1861 ttcctaatac cgtactgatg ccctgtgcca tcgtttcggt gacgggttga gagagcgtgt
     1921 cagtctcgaa gccctgtcgg atcagccatt ccaccagcag cttgcgttca ctccccagcc
     1981 cgaggtcgag gcgggtgaca aaagcggatt gtgcgttggt gcttggggtg agcgccaaca
     2041 aaaagaaact gaataaaaag tattg
//
LOCUS       DAOTON010000546         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_42064_length_2065_cov_2.190050, whole genome shotgun sequence.
ACCESSION   DAOTON010000546 DAOTON010000000
VERSION     DAOTON010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42064_length_2065_cov_2.190050"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="P5292_10210"
     CDS             complement(<1..385)
                     /locus_tag="P5292_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRS39541.1"
                     /translation="MLSRSLLFAGLAVLLFSGTALARTFRVLFIGNSYVAVNDLPNQL
                     RNFCLAAGDTLIVDSSAPGGYTFEAHSTDATTLQKIAAGGWDFVILQEQSQRPAFPPS
                     QVEQEVYPFAQVLDSLIHVSNPCAKT"
     gene            complement(385..>2065)
                     /locus_tag="P5292_10215"
     CDS             complement(385..>2065)
                     /locus_tag="P5292_10215"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS39542.1"
                     /translation="AVMNTRVYRSVNNGSSWTNISTGLPSVNWNDIVIDETFPAQELV
                     FVAASNSVYYKKAASSSWTNYSTGLPARTNINDITIFENGTSQALMRVSTYGRGMWET
                     SFENLRPLQAVLTSDKTELCPGGTVQFSDLSIGLPTSRTWTFPGGTPSSSTSLNPSVT
                     YSSPGKYAVTLSVSNGSGTSSVTKTAYITTLGKNLPVIETMESTAFPPANWSLYDDGA
                     DQRVWKRYGSGSSYGVGFSSAVFDNWNYNAQGKRDELRTFPIDLTEYATARLIFDVAY
                     QLYTNPSYVDSLLIKVATNCGTNYQTVYLKPGPTLATIPTIGTSAYYPAAGDWRTDTV
                     NLNAFAGQSNVIASFVNIGRYGNYLYIDKVRVEGDHVPVNLQLKTFIQGFYLGANQQV
                     AALNPAGAPTVCDSITVLLASATSPYAFTDTVKSLLMTNGTVTATFPGKVYGNQYYIV
                     LRQRNSLETWSAVPITLDATNVSYDFTLSASKAYGNNQQVIGGVATLYSGDVNQDGLI
                     EASDYALEENGVSQFLFGYVREDLTGDQLVEAADYSLIENNLLLFLFTSRP"
BASE COUNT          484 a          528 c          608 g          445 t
ORIGIN      
        1 cggtctttgc acagggattg ctcacgtgaa tgaggctgtc gagtacctgt gcaaacggat
       61 aaacctcctg ttccacctgg gaaggcggga atgccggacg ctggctttgc tcctgaagaa
      121 tgacgaaatc ccaaccaccg gcggcgatct tctgcaacgt ggtggcatcg gtgctgtgcg
      181 cctcaaaggt gtagccgccg ggagcgctgg aatcgacgat cagtgtatcg cctgcggcca
      241 gacaaaagtt acgcaactgg ttgggaaggt cgttcacggc tacatagctg ttgccgatga
      301 acaagacacg aaacgtacgg gccagggcag ttccggaaaa cagcaataca gccaggccgg
      361 cgaagagcaa cgatcgggaa agcatcaggg tcgggaggtg aagaggaata agaggaggtt
      421 attttcgatg agggagtaat cggcagcttc taccagttgg tcgccggtga ggtcttcgcg
      481 cacatacccg aagagaaact gactgacacc attctcttca agggcataat ccgaagcctc
      541 gatcagaccg tcctggttca catcaccact gtaaagtgtc gccacaccgc cgatcacctg
      601 ctgattattc ccgtaagcct tcgatgcgga tagcgtgaaa tcatagctca cgttggtggc
      661 atccagggtg atggggacgg cactccaggt ttcaagggaa ttgcgttggc gcagtacgat
      721 gtaatactga tttccgtaca cctttccggg aaacgtggcg gtaaccgtac cgttggtcat
      781 gagcagcgac ttgacggtat cggtaaacgc atacggactg gttgcagatg ccagcagaac
      841 agtaattgag tcgcaaacgg ttggcgcacc ggcaggattc aaagccgcca cctgttgatt
      901 cgctccgagg tagaaaccct ggataaaggt cttgagctgg agattgaccg gaacatggtc
      961 cccctctacc ctcaccttgt cgatgtaaag ataatttccg taacgtccaa tgttcacaaa
     1021 gctggcgatg acgttgctct gtccggcaaa ggcatttagg ttgaccgtat ccgtccgcca
     1081 gtcgccggcg gctggataat aagcagatgt accgatcgtg ggaatcgtag ccagagtcgg
     1141 ccccggtttg agatacacgg tctggtagtt agttccgcaa ttcgttgcca ctttgatgag
     1201 caacgagtcc acgtaagacg ggttggtata caactgatac gccacatcga atatgaggcg
     1261 ggcggtggca tactccgtca ggtcgatcgg aaaggtccgc aattcatccc gcttgccttg
     1321 cgcgttgtaa ttccagttgt cgaacacggc ggaactgaag ccaacaccat acgaggaacc
     1381 ggagccgtaa cgtttccata cacgctggtc tgcaccatcg tcgtagagcg accagttcgc
     1441 cggaggaaaa gcggtgctct ccatcgtttc gatcaccggc aaattcttac cgagtgttgt
     1501 gatgtaagcg gttttggtga cactgctggt cccggaaccg ttgctgacac tgagcgtcac
     1561 ggcatacttt ccgggtgagc tgtaagtgac cgaagggttc aacgaagtgc tgctggacgg
     1621 agtaccgccg gggaaggtcc aggtcctgct ggtcggcagt ccgatcgaga gatcggaaaa
     1681 ctgcacagta ccacccggac acaactcggt cttgtcggag gtcaggacag cctgcaacgg
     1741 acgcaggttc tcgaacgatg tttcccacat accacggcca taggtcgata cccgcatcag
     1801 ggcctgactg gtgccgttct cgaagatggt aatatcgttg atgttggttc gggcaggcag
     1861 gccggtgctg taattggtcc aggaagagga agcggccttc ttgtaataga cgctgttgct
     1921 cgcggccacg aagaccagtt cctgtgcagg aaaggtttcg tcgatcacga tatcgttcca
     1981 gttgacggaa ggaagaccgg tactgatatt ggtccagctc gacccgttgt tcactgagcg
     2041 atagacccgc gtgttcatga cggca
//
LOCUS       DAOTON010000547         2063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_42109_length_2063_cov_3.738546, whole genome shotgun sequence.
ACCESSION   DAOTON010000547 DAOTON010000000
VERSION     DAOTON010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42109_length_2063_cov_3.738546"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..617
                     /gene="purE"
                     /locus_tag="P5292_10220"
     CDS             117..617
                     /gene="purE"
                     /locus_tag="P5292_10220"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRS39543.1"
                     /translation="MSNPLVGIIMGSQSDLSVMQDAAQLLTEFGIAHEVRIVSAHRTP
                     EWMMDYAKTAADRGLRVIIAGAGGAAHLPGMVASITPLPVIGVPVRSSNSIDGWDSIL
                     SILQMPNGVPVATVAINAAQNAGILAAQILGAADPEVRKKVLEFKNSLREKVMNANDA
                     LKKGSS"
     gene            697..1509
                     /locus_tag="P5292_10225"
     CDS             697..1509
                     /locus_tag="P5292_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39544.1"
                     /translation="MSYRLILLAACLALANGVLASNIRSWGMATSGVSLSDEIAILTN
                     PASPGDTSASTVQAYVEDRYGIGLLRNCGLAFATAAGNRRIGMEVTQTGKAPYRNNQV
                     NLSAGITLFNKSRFGISLRAYHRSVREQQQTTSILTWGSSLGWWIQPFPKTSLGLVID
                     HPESMTRTISTNVEQPSIRCGLSWSPLRAINWSLEAVQRSGTPIVVASGIRYQATSML
                     GFSCGWRSDRMLLSLGSSVSFSTWRLDFYFEQHRQLGTRTGAALLFFLHKRS"
     gene            1506..>2063
                     /locus_tag="P5292_10230"
     CDS             1506..>2063
                     /locus_tag="P5292_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRS39545.1"
                     /translation="MRRPEGLLTLLMLAAIYSVRAQDSLQEMKPIEAIVWREDGTDRD
                     GADAMEEISLRMSQRIDLNTASLEHLKELPGLTNEQVERMIGHRERFGPILRLEELQV
                     IASLSPDDIRRIAPYIRITDRQTFAIKQAQLRQELIVRLGGAGTVWNGTEQEYKKTAG
                     NGFRSLLRYRMRIRDQWTMGLRMEKD"
BASE COUNT          498 a          549 c          567 g          449 t
ORIGIN      
        1 aaaatggggc atgttacagt ctgccatccg gacctggaag aagcaagacg tattgccaga
       61 accatacgtg atacactgaa agtaaaagca taacgtccac atatccaccc agcgcaatgt
      121 ccaatccact tgtaggtatc atcatgggaa gccagtccga cctctccgtc atgcaagacg
      181 cggctcaact gttgacggaa ttcggcattg cccatgaggt caggatagtt tccgctcatc
      241 ggacaccgga gtggatgatg gattatgcta agacagcggc cgaccggggc ctcagggtta
      301 tcatcgcggg tgccggtggc gccgcccatc ttccgggcat ggtagcttcg ataacacccc
      361 tgccggtaat tggagttccg gtgcgatcaa gcaattccat cgacggctgg gactcgatcc
      421 tttccatcct ccaaatgccc aatggagttc cggttgcaac ggtagcgatc aacgcggcac
      481 aaaatgcggg tatactggcc gcgcagatat tgggagctgc tgatcccgaa gtcagaaaaa
      541 aggttctgga attcaagaat tcactccgtg aaaaggtgat gaatgccaat gatgccttga
      601 aaaaagggtc ctcctaagtg ccggggcacc tccgcatata atcaaccgca tttacagttt
      661 ctttccgaca aacgaccccg atagtttcgc ggtcgaatgt cctatcggtt gatccttctc
      721 gcagcgtgtc tcgcccttgc caacggtgtt cttgcatcga acatccgctc ctggggaatg
      781 gccacgagcg gcgtttcgtt gagtgacgag attgccattc tcacgaatcc cgcttctcct
      841 ggagatacca gcgcttccac cgtgcaggcg tatgtggaag atcgctatgg gatcggcctg
      901 ctgcggaatt gtgggctggc tttcgctacc gcagcaggaa atcgccgcat aggaatggaa
      961 gtcacccaga ccgggaaagc accttatcgc aacaaccagg ttaacctttc tgccggaatc
     1021 acacttttca acaagtcccg cttcggcatc tcgctcagag cttaccaccg atctgtgcgt
     1081 gagcagcagc agaccacgtc gatcctgacg tggggctcgt ccctggggtg gtggattcag
     1141 ccatttccaa agacgagcct tggcctggtc atcgatcacc ctgaatccat gacacgaacg
     1201 atctccacca atgtcgagca gccaagtatt cgctgcggat tgtcctggag ccctttgcgg
     1261 gctatcaact ggtctctgga agctgtccaa cgctcgggaa cgcctattgt agtcgcatcc
     1321 ggtatacggt atcaggccac gagtatgctg gggttttcct gcggctggag atcggatcgc
     1381 atgctactga gcctgggtag cagcgtttcg ttttcaacct ggagattgga cttctacttc
     1441 gaacaacatc gtcaactggg aacgcgtacc ggtgcagcgc tgctgttctt tctccacaaa
     1501 cggtcatgag aaggcctgaa ggattgctga ctttactgat gcttgccgcg atctacagcg
     1561 ttcgggcaca ggatagtctg caggaaatga agccgattga agcaatagtc tggcgcgaag
     1621 acggaaccga ccgtgatggc gcggacgcga tggaagagat cagcctgagg atgagtcagc
     1681 gcatcgatct gaataccgcg tccctggaac acttgaaaga actaccgggt ctgacgaatg
     1741 agcaagtgga acgaatgatc ggccatcggg agcgtttcgg accaattctt cgtttggaag
     1801 aactccaagt aattgccagc ctgtcacccg atgacatccg gcggatcgcg ccctatatca
     1861 gaatcacgga tcggcaaacc tttgccatca agcaagccca gctacggcag gaattgatcg
     1921 ttcgccttgg aggagcagga acggtttgga atggaacaga acaagagtac aagaaaaccg
     1981 ccgggaacgg atttcgaagt ctcctgcgct atcgtatgcg aatcagggac cagtggacaa
     2041 tgggattacg gatggagaag gat
//
LOCUS       DAOTON010000548         2054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_42382_length_2054_cov_3.187594, whole genome shotgun sequence.
ACCESSION   DAOTON010000548 DAOTON010000000
VERSION     DAOTON010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42382_length_2054_cov_3.187594"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="P5292_10235"
     CDS             <1..558
                     /locus_tag="P5292_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRS39546.1"
                     /translation="ERITSTKRGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLNR
                     KIAELGIYPAVDPLDSTSRILSPAVVGAEHYNTAQRVKMLLQRYKELQDIIAILGMDE
                     LSDDDKLVVHRARRVQRFLSQPFNVAEQFTGIKGVIVPIEETIRGFNMIMNGDVDEYP
                     EAAFNLVGTIDDAIAKGKKILAESK"
     gene            665..904
                     /gene="atpC"
                     /locus_tag="P5292_10240"
     CDS             665..904
                     /gene="atpC"
                     /locus_tag="P5292_10240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRS39547.1"
                     /translation="MYLEIITPDKKVYAGEVSSVAVPGTNGSFQVLKNHAPIISSLLN
                     GKVKVKDKEGEKVFSVKGGVVENLNNKVVILAESV"
     gene            1323..>2054
                     /locus_tag="P5292_10245"
     CDS             1323..>2054
                     /locus_tag="P5292_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39548.1"
                     /translation="MPLRLLFLLVVCSLQAGAQFTDRYWTFGDSAGIDFVNPSNPQTG
                     LSSLRSRGSCVSICDSAGQLLFYGGTPNTSLWPQTIVQELGLLFNKNNLLMENGDTLV
                     GGAWYQEMVIVPWPNHPGLFYIFHVGITSTTDPGLYYSMVDLSYNGGLGKVIQKNVQL
                     QNFPVNDGLAAVKHGNGRDWWVVHKRYEPNVFTDEFYLYLVTASGVQPQPIQFTGRPS
                     KNNNLRIKFSPSGNKMAVHEAGNNLD"
BASE COUNT          506 a          550 c          511 g          487 t
ORIGIN      
        1 gagcgcatca cctccaccaa gcgtggttcg atcacctcgg tacaggcggt ttacgtaccg
       61 gccgacgact tgaccgaccc ggctccggcg accaccttcg ctcacctgga tgccaccacc
      121 gtattgaacc gtaagatcgc cgaactcggc atctatccgg ccgttgaccc gctggattca
      181 acctcgcgta ttctctcccc ggcggttgtc ggcgccgagc actacaacac cgcccagcgc
      241 gtgaagatgc tcctccagcg ctacaaggaa ctgcaggaca tcatcgcgat cctcggtatg
      301 gatgaattgt cggacgacga caaactcgtc gtacaccgcg cccgccgcgt gcagcgcttc
      361 ctctcgcagc cgttcaacgt ggccgagcag ttcaccggta tcaaaggcgt gatcgttccg
      421 atcgaagaga ccatccgtgg tttcaacatg atcatgaacg gcgacgtcga cgaatatccg
      481 gaagccgcct tcaacctcgt cggcaccatc gacgacgcga tcgcaaaagg caagaagatc
      541 ctcgccgaaa gcaagtaatt atcgttgaac gttggaaagt tgaacgttga aagtttggtt
      601 accatccgga gctacttaaa ctttcaacct tcaactttca accttcaacc ttcaactaac
      661 agacatgtat ctcgaaatca tcacccccga taaaaaggtc tacgccggcg aagtgagctc
      721 ggttgccgta cccggcacca acggcagctt ccaggtcctg aagaaccacg caccgatcat
      781 ctcctccctg ctcaacggca aggtgaaggt gaaagacaag gaaggcgaaa aagtcttttc
      841 ggtaaaaggc ggtgtcgtag aaaacctcaa caacaaagtg gtgatcctcg ccgagtcggt
      901 ctgatccgct gcttcaaatc ttcagaaagc cttcccgttt gggagggctt ttttgttttc
      961 atattccggg cttcgaatcg gaggcacacg gactacacgg ataagacgga tgctcacggg
     1021 aatatatccg cgcaaatccg caacatccgc ggaatccgcg tgccatcaat ttgtgaccac
     1081 tttcggtagg cataggatta gtagcacagc gtataccaga ggacacacag cgtcccacag
     1141 agatcgaact ctgcgcgact ctgcgtgccc tctgcgtgac tccgtgtaac taaaaactac
     1201 gaagcaggca ggtgaatcac acatctccgc gggctatcaa tatttgaccc tcatcagatt
     1261 ccgcgattcg ccgaaagttc cgggatttcg tatcttgaca tactgctatc aagctttccc
     1321 gcatgccgct ccgcctttta ttcttgctcg tggtgtgttc cctacaggcc ggggctcagt
     1381 tcaccgaccg ctactggacg tttggcgaca gcgcggggat tgatttcgtc aatccgagca
     1441 atccacagac gggtctttca tcgctccgat ctcgtggaag ttgtgtgagc atctgtgact
     1501 ctgctggtca actcttgttc tatgggggaa ctcctaatac ttcgctgtgg cctcaaacca
     1561 tagttcagga attgggcttg ctgtttaaca agaataattt gttaatggag aatggtgata
     1621 ccttagttgg cggagcatgg taccaggaga tggttattgt gccgtggccg aatcatccgg
     1681 gcttgtttta tatttttcat gttggaatta cctctacaac agatccggga ttatattatt
     1741 cgatggtcga cttaagctac aacggcggct tgggaaaagt tattcagaag aatgtgcagc
     1801 tacagaattt tcctgttaac gatggacttg cagcggttaa acacgggaat gggcgggact
     1861 ggtgggtggt tcacaaaagg tacgagccca atgtgtttac agatgagttt tatttgtatt
     1921 tggtaaccgc gtcaggagtt cagccacagc cgatccaatt taccggtcgg ccttcaaaga
     1981 ataacaattt aagaattaag tttagtccca gcggaaacaa aatggcggtt catgaagcag
     2041 gcaacaatct tgat
//
LOCUS       DAOTON010000549         2047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_42594_length_2047_cov_2.536647, whole genome shotgun sequence.
ACCESSION   DAOTON010000549 DAOTON010000000
VERSION     DAOTON010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42594_length_2047_cov_2.536647"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2047)
                     /locus_tag="P5292_10250"
     CDS             complement(<1..>2047)
                     /locus_tag="P5292_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRS39549.1"
                     /translation="HRQQIVHLAESRLLPLLRKAIEIQDRDLRRYATGFQLLKTLYLF
                     GIVNDLSLHYRDYRNENNKILISDTPRLLGAVMQESDAPFIYEKVGTRYKYLLIDEFQ
                     DTSLIQWQNILPLLEHTLGSGFMALVVGDAKQSIYRWRGGDMSLLLQGIRRDLRAFGS
                     LFMEDVLDTNYRSKREIVLFNNSFFEKVPPLLEEVKEAGALPLLRQAYGAGLAQAVAK
                     KNDIGGFVRIRPLTASTDPATGEERTWKAAAIEATADTIGQLLKRGYQLRDITLLVRN
                     NRDGNLLATELFQRGFRDIISPDSLLLGTHPLIQFLLNVFRFLADPGNSIAKSQLLHY
                     YVTQLREQDGVSLHRLFADHQLKSRKRPSATISLFDSEVHTDNAFNELLPDSFTAHLP
                     YLSKLPVYELSEQLAAIFHLNSEPDAYLQRFQDLALEFTQNDSGNLAAFIAWWDENEI
                     GLTKSVVVPEDVNALRIMSIHKSKGLQFPIVIMPFTDWSLFPKANEWIWATTDEPPFD
                     QIGKVALNTGKKLLGTAYEAAYREEIQQQLVDNTNLLYVAFTRAEHELHIFLPEDSGG
                     ELSKVSQLVSRTCDSMDKLMRSGEEWVAGEPMPSPEASPAGTKTEVLRSYPIHRWQER
                     ISIASKARDLTGMWEEEKTSGKRFGIIVHRLLSEITATSDIERAVQAQVFDGLLREEE
                     "
BASE COUNT          413 a          529 c          622 g          483 t
ORIGIN      
        1 cttcctcttc ccgaaggagt ccgtcaaaca cctgtgcttg tacggcccgt tcgatgtcgg
       61 acgttgcggt gatctcagaa agcaggcggt ggacgatgat gccgaatcgt tttccactgg
      121 ttttttcttc ctcccacatg ccggtgagat cacgtgcttt ggaggcgatg ctgatccgtt
      181 cctgccagcg gtgaatggga taggagcgga gtacctcggt cttcgttccg gcaggcgatg
      241 cttccggcga tggcatcggt tcgccggcta cccattcctc accggaccgc atcagcttgt
      301 ccatgctgtc gcaggtcctg ctgaccaact gcgatacttt gctcagttcg ccgccggaat
      361 cttccggaag gaatatgtgc aattcgtgtt cggctcgggt gaacgcgacg tagagcagat
      421 tggtgttgtc gaccaattgc tgttggatct cttcccggta cgcggcttca tacgcggtac
      481 caagaagttt ctttccggtg ttcagcgcta ctttcccgat ctgatcgaac ggaggttcgt
      541 cggtcgttgc ccatatccat tcgttcgcct tggggaaaag tgaccagtcg gtaaagggca
      601 tgatgacgat cgggaactgc agtcctttcg acttgtggat cgacatgatg cgcaaggcgt
      661 tcacatcctc cgggaccacc accgatttgg taagaccgat ctcgttctcg tcccaccagg
      721 cgatgaatgc ggccagattg ccggaatcat tctgcgtgaa ttcaagtgcc aggtcctgga
      781 atcgctggag ataggcatcg ggttcgctgt tgagatggaa gatcgcggcc agttgttcgc
      841 tcagttcata aacgggaagc ttggacagat agggcaggtg cgcggtaaag gaatccggaa
      901 gtagttcgtt gaacgcattg tcggtatgga cttccgaatc gaagagcgaa atcgttgcgg
      961 acggtcgttt gcggcttttc agctgatggt cggcaaaaag ccgatggagc gagacgccgt
     1021 cttgttcccg taactgggtg acatagtagt gcaacaattg gctcttggcg atgctgttgc
     1081 cgggatcggc aaggaagcgg aatacgttca gcagaaactg aatcaacggg tgcgttccca
     1141 ggagcaatga atccggagaa atgatgtcgc ggaagccccg ctgaaaaagt tcggtggcga
     1201 gcaggttgcc gtcgcggttg ttgcgcacta agagggtgat gtcgcgcagc tgatagccac
     1261 gtttcaataa ttgcccgatg gtatcggcag tcgcttcaat ggcggcagct ttccaggtcc
     1321 gttcttcacc ggttgccgga tcggtggagg cggtgagcgg gcgaatccga acaaaaccgc
     1381 cgatgtcgtt tttcttcgca acggcctgtg ccagtccggc tccataggcc tgccgcagca
     1441 gcggcaaagc gccggcttct ttcacctctt cgagcagcgg tggtactttt tcgaagaaac
     1501 tgttgttgaa caagacgatc tcgcgtttac tgcgatagtt cgtgtccagt acatcttcca
     1561 tgaagagcga gccgaacgcc cgcaggtctc gccggattcc ctgtaaaagc aatgacatgt
     1621 cgccgccccg ccagcggtaa atggattgct tggcatcgcc caccacgagc gccatgaagc
     1681 cgctgcccag cgtgtgttcc aacaggggca ggatgttttg ccattggatg agggaggtat
     1741 cctggaattc atcgatcagc agatacttgt agcgggtgcc gaccttttca tagatgaacg
     1801 gggcgtccga ttcctgcatc acggctccca acaggcgagg ggtatcggag atgaggatct
     1861 tattgttctc gttccggtaa tcgcggtagt gcagggaaag gtcgttgaca atcccgaaga
     1921 ggtacagcgt cttgagcagt tggaaaccgg tggcatagcg ccgcaggtcc cggtcctgga
     1981 tctcgatcgc tttgcgcagg agtggcagca ggcgactttc agccaggtgc acgatctgct
     2041 gccggtg
//
LOCUS       DAOTON010000550         2032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_43015_length_2032_cov_2.891249, whole genome shotgun sequence.
ACCESSION   DAOTON010000550 DAOTON010000000
VERSION     DAOTON010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43015_length_2032_cov_2.891249"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="P5292_10255"
     CDS             complement(<1..657)
                     /locus_tag="P5292_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRS39550.1"
                     /translation="MLSVEGITVWVEEGLNRVFPAAVALVLEFAIVGLLMIGLFAVLG
                     LVLVFMERKVSAYMQVRVGPNRVGYRGSLQTLADTLKLLVKEGLTPDGADKFLFNFAP
                     FLVMIASMLLMAPIAFARNVQIWDLNIGVLYVTAVSSIGVIGILMAGWSSNNKYSLLG
                     AMRSGAQIVSYELSAGLAILTIVVLTGSLRISDIIASQADGWWIFKGHIPAIIAFVIF
                     I"
     gene            complement(816..1931)
                     /locus_tag="P5292_10260"
     CDS             complement(816..1931)
                     /locus_tag="P5292_10260"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRS39551.1"
                     /translation="MLEEVGTTDQGDLIINVGPQHPSTHGVLHLVITLNGETIRKIEP
                     HLGYIHRSIEKMCESLSFRQFIYSTSRMDYLSSHINNHGCALAVEQGLGIEIPERAQV
                     IRVIMDELTRLASHQLWWGAMAMDVGAITPFFHAFREREMINDIMEETCGARLTMNYM
                     VPGGVMFDLHPNFQRKVREFLRFFESKFDEYDELVTHNVIFQSRMKGVGVLSKADAIS
                     FGCTGPVARASGVSSDIRKLYPYDCYSKVQFDEVLEKDGDCFARYLVRMGEMKQSVRI
                     LEQLIDNIPDGDFQAKTKAVLKLPKGEYYSTVETARGEFGVYIISEGGMTPYRIKFRS
                     PGFSNLSALDHMARGSKIGDMVAIMGTLDLVIPDIDR"
BASE COUNT          484 a          626 c          472 g          450 t
ORIGIN      
        1 gatgaagatc acaaacgcga tgatcgccgg gatatgccct ttgaagatcc accagccgtc
       61 ggcctggctg gcaatgatgt cggaaatacg cagactaccg gtcagcacca cgatcgtgag
      121 gatcgccaga ccggctgaaa gttcatagct gacgatctgt gccccgctgc gcattgcgcc
      181 cagcagggag tatttattgt tgctggacca gccggccatc aggatcccga tcaccccgat
      241 cgacgaaact gcagtcacat acaaaacacc gatgttgagg tcccagatct gcacgttgcg
      301 ggcgaaggcg atcggcgcca tcagcaacat cgaagcgatc atcaccagga aaggcgcgaa
      361 gttgaagagg aatttatctg caccgtccgg cgtcaggcct tccttcacca gcaatttcaa
      421 ggtgtcggcc agggtctgca gactacctcg ataccccacc cgattgggcc cgacccgcac
      481 ctgcatatag gccgaaactt tccgctccat gaataccaat accaacccca gcacggcgaa
      541 gagcccgatc atcagcaagc cgacaatcgc gaactccaat accaaagcca ccgcagccgg
      601 aaagacccgg ttcagtcctt cctctaccca aacggtaatg ccttcaacgg ataacataac
      661 ggagcaagtt tcgcgtttcg agtttcgagt ttcgggtttc gggtttcggg tttcccgcct
      721 tcgcaaaagc ttcggcaggc actattcgct attcgctatt cactattcac tattcactgt
      781 tcactgttta ctgttcacta ttcactgcca actcctcacc gatcaatatc cggtatcacc
      841 aaatccaacg tccccatgat cgccaccata tccccgatct tcgatccgcg ggccatgtga
      901 tccaaggccg acaggttcga gaatcccggc gaacggaact tgattcgata cggggtcatg
      961 cctccttcac tgatgatata gacaccaaat tcaccacgcg ccgtctctac ggtcgaataa
     1021 tactcgcctt tcggcaactt caacaccgcc ttggttttgg cctggaagtc accgtccggt
     1081 atgttatcga tcaactgttc gagaatgcga accgattgct tcatctcgcc catgcggacc
     1141 agatagcggg caaaacaatc gccgtctttt tccaggactt cgtcgaattg cacctttgag
     1201 tagcaatcgt acgggtacag tttccggata tcggaagaga caccggaggc acgtgccacc
     1261 ggaccggtac aaccgaagga gatcgcgtcc gctttcgaca atacacccac tcccttcatc
     1321 cgcgactgga aaatcacgtt atgcgtgacc aactcgtcgt actcgtcgaa ctttgattca
     1381 aagaatcgga gaaactccct gactttccgc tggaaattcg gatggagatc gaacatcaca
     1441 ccgcccggca ccatgtaatt catggtcaag cgtgcgccgc aggtctcttc catgatgtcg
     1501 ttgatcatct cccgctcccg gaaggcgtgg aagaacggcg tgatcgcccc cacgtccatc
     1561 gccatggcgc cccaccacaa ctggtgcgat gccagacgcg tcaactcgtc catgatcacc
     1621 cggatcacct gcgcccgctc cggaatttca atacccaacc cctgctctac cgccagcgca
     1681 catccgtgat tgttgatgtg cgacgacaga taatccatcc gcgaggtcga gtagatgaac
     1741 tgccggaaac tcaggctctc acacatcttc tcgatcgacc ggtggatgta acccaaatgc
     1801 ggttcgatct tccggatcgt ctccccattc agcgtaatga ccagatgcaa caccccgtgc
     1861 gtagaaggat gctgcggccc cacattgata atgaggtccc cctgatcagt cgtgcctact
     1921 tcttcaagca tacaagtggg ttccgggttc tgagtttcgt gtttcgagtt tcgtgtttca
     1981 agattcctgt cttccctaaa acatcagcag gcagaattca ctattcacta tt
//
LOCUS       DAOTON010000551         2018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_43416_length_2018_cov_3.210392, whole genome shotgun sequence.
ACCESSION   DAOTON010000551 DAOTON010000000
VERSION     DAOTON010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43416_length_2018_cov_3.210392"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1561)
                     /locus_tag="P5292_10265"
     CDS             complement(98..1561)
                     /locus_tag="P5292_10265"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HRS39552.1"
                     /translation="MKIRILLLAMLWSTMAAAAGRVKAYLDLAAFDSPGQSPYVESYL
                     NVVGNSVRLRQLSDGKFQASIEVQWIFSKEEQIVYFDKYNLLGPAVDTSVISVSPDFI
                     DQVRVPLAPGEYKLEVRIRDNNSDGEPVSVVQPLTLRFPDKQVGISDIEFLESIRTSD
                     GKNKFSKSGFDLIPNVHSYYPKDIQTLRFYAEVYRSKELIGGDFLVRYFVVQHESNAI
                     QTDFAGFKRVSAADVVPVMIEMPIDKLLSGNYQLVVEVRDRENKVLGQRTAFFQRSNV
                     LEKPVVTDNISAIPVEGTFASLLSYEDTLRQAIDCLYPISSQLELQIAETQLKISDIR
                     SMQQYVNYFWSRHAPEDPEKGWREYQLEVQKVNASFGTRNMKGYETDRGRVYLQYGPP
                     NTIVTETTDPNSYPYEIWHYYKAGDQTNRKFVFYSTELSANVYRLLHSDARGELQEPN
                     WELKLHSRSQQFGVDMDQGNSFDTYGSQTKENFRMPK"
     gene            1709..>2018
                     /locus_tag="P5292_10270"
     CDS             1709..>2018
                     /locus_tag="P5292_10270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS39553.1"
                     /translation="MFFFLLSVFLLSAGLICYSYVLFPLYLAIRSRNKSSGTVQFTRD
                     AAALPGISFIIAAYNEERVIAEKLEALLASDYPEDRLEILVGSDASVDLTNALLTGFA
                     N"
BASE COUNT          473 a          490 c          555 g          500 t
ORIGIN      
        1 aggatgacga cagcgacgcg cgcaggctga ttggacatac gaatgaaaaa cccctgcaaa
       61 gatgcaaggg tttttggaat ggcctaacag gctcggatta cttcggcata cggaagttct
      121 ctttcgtctg actgccgtag gtatcgaacg aatttccctg gtccatatcg acgccgaact
      181 gctgtgaacg gctgtgaagt ttcagttccc agttcggttc ctgcaattct ccccgggcat
      241 cggaatggag cagacgataa acgttcgccg acagttcggt cgagtagaac acgaacttcc
      301 ggtttgtctg atcaccggcc ttgtagtaat gccagatctc gtaagggtag gagttgggat
      361 cggttgtttc ggtgacgatc gtattcggag gtccgtactg gagataaacg cgtccacggt
      421 cggtttcata acctttcatg ttccgtgtac cgaaggaagc gttcaccttc tggacttcca
      481 attggtattc ccgccagcct ttttccggat cctcgggtgc atgacggctc cagaaatagt
      541 tgacgtattg ttgcatcgac cggatatcgg agatcttcaa ctgggtctcc gcgatctgta
      601 attcgagttg ggaagaaatc ggataaagac aatcaatggc ttggcgaagc gtgtcttcat
      661 aactcaacag cgaggcaaac gtgccttcaa ccggaatagc ggagatgtta tcggtgacga
      721 ccggtttttc cagcacattg cttcgctgga aaaaggcggt gcgctgtccg aggactttgt
      781 tttcccggtc gcgcacttcg acaaccaact gataattgcc ggacaagagt ttatcaatcg
      841 gcatttcgat catgaccgga actacatcgg cagcggagac gcgtttgaag ccggcgaagt
      901 ccgtttgaat ggcgttggat tcatgctgta caacaaagta gcgaaccagg aagtcgccgc
      961 cgatcagttc tttggaacga tatacttcgg catagaaccg aagggtctgg atgtccttgg
     1021 ggtaatagga atgaacattc ggaatgaggt cgaaaccgga cttcgagaat ttattctttc
     1081 cgtcggaagt tctgatcgat tccaggaatt cgatatccga gatgccgact tgtttgtcag
     1141 gaaaacggag ggtcaacggc tgaaccacgg agaccggttc gccatcggag ttgttgtcac
     1201 gaatgcggac ctccagtttg tattcgccgg gagcaagcgg aacgcgaacc tggtcgatga
     1261 aatcggggct tacactgata accgaagtgt cgaccgctgg tccgaggaga ttgtacttgt
     1321 cgaaatagac gatctgctct tccttgctga agatccactg gacttcaatg gaggcctgaa
     1381 acttcccgtc gcttagctgc cggagcctga cggaattgcc caccacgttg agatacgatt
     1441 cgacataagg cgactgaccc ggtgaatcaa acgcggccag gtccaggtaa gccttgacac
     1501 gacccgctgc ggcggccatc gtggaccata gcatggcaag taacaggatc ctgattttca
     1561 tggcggttgg ggtatgctgt aaagatacga aaggggctgg ggttcagtag ggaagacggc
     1621 ttttttaaca agggtgtgac gaaaggagac gacatcttca cccgatagaa accgcctgag
     1681 tttgactatt tttacgcgcc agaaccgcat gtttttcttc ctgctttccg tttttctgtt
     1741 gagtgccgga ctaatctgtt actcgtatgt actatttccc ctttacctgg cgatccggtc
     1801 gcgtaataag agttccggta cggttcagtt taccagggac gcagcggcac ttccgggtat
     1861 ttccttcatc atcgccgctt acaacgagga acgcgtcatt gcagaaaagc tcgaagccct
     1921 cctggcctcc gattaccccg aggatcggct ggagatactc gtaggctccg atgcatcggt
     1981 tgacctgacg aatgcgttgc ttactggatt tgcaaacc
//
LOCUS       DAOTON010000552         2017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_43451_length_2017_cov_2.750255, whole genome shotgun sequence.
ACCESSION   DAOTON010000552 DAOTON010000000
VERSION     DAOTON010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43451_length_2017_cov_2.750255"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..>2017
                     /locus_tag="P5292_10275"
     CDS             53..>2017
                     /locus_tag="P5292_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRS39554.1"
                     /translation="MLSGSVLNVIPVSCNGGTDGGISVGITGGAGSYAYQWSPSVSTG
                     PSAGGIGQGQYSVIVTDAHGCTVALSAAVPEPPPLVTTATPIVQVLCYGGQDGSASVS
                     ASGGTAPYTYLWSPTGQTTALATGLAAGNYTVVVRDANGCQRTESMQITEPSLLTLSL
                     ASTPSTCGSANATANATPGGGTAPYSINWTPGGSSSVSLSGIFSGNYTSVVTDANGCT
                     TSSSILINDLPGPVASLASSTDVSCNDGQDGAASVTISGGSSPITYQWLPTGGSSSSA
                     TGLSAGQYSVIITDANNCVQTVGLTISEPALLTSAAAASQMVSCYGLSDGAASVNVTG
                     GTAPYRYLWSPCGSTTSQITNSTAGNYSVVVTDAMGCTTTAATTITEPSLLTASTSAT
                     QSTCGNADGSASVSANGGSGGYAYLWQPGNSTQPTAGGLAAGAYTVTITDANGCTTTA
                     IAAVSDMGAPVIQPGPIIPVNCPGGSDGSTSVTLTGGTAPFQYQWSGGVASGNAVSGL
                     SAGAYSIVVTDGNQCVAVQSFVITEPPPIELSSLSLPVSCHGGNDGALQLTTGGGTPG
                     YSYLWSNGATSQTATMLTTGNYSVVVTDAHGCTSSLSNVITEPDAITASTSSVPVLCN
                     GGVDGAAEIAVNGGTPPYNYEWAGIGVQQPL"
BASE COUNT          410 a          621 c          572 g          414 t
ORIGIN      
        1 ggacgcgaac ggttgtacga gttcttcgaa tgtgcagatc accgaaccgc ctatgctgtc
       61 gggatccgta ctcaacgtga ttccggtctc gtgtaatggt ggtaccgatg gcggcatctc
      121 ggtcggcatt accggaggag caggttcata cgcctatcaa tggtcgcctt ccgtctccac
      181 gggcccctcg gccggtggta taggacaagg tcaatactcg gtaatcgtca ccgatgcgca
      241 tggttgtacg gttgctctct cggccgcagt gccggagccg cctcctttgg taacaaccgc
      301 tactccgatt gtgcaggtgt tatgctacgg aggacaggac ggcagcgcga gtgtttcggc
      361 ttccggcgga acggctcctt atacctatct gtggagtccg acgggccaaa cgaccgcgct
      421 ggcaacagga ttggccgcgg gtaactacac cgtcgtcgtt cgagatgcga acggatgtca
      481 acgaaccgag tcgatgcaga tcacggaacc ttccctgttg acattatcgc ttgcatcaac
      541 accgtccact tgcggatccg ccaatgctac ggcgaatgca acacccggtg gaggcacagc
      601 cccctattcg atcaactgga cgcccggcgg atcatcgtcc gtctcgctta gcggcatctt
      661 ttccgggaat tacacctcgg tggtcaccga tgcgaacggc tgtacgacga gttcaagcat
      721 cctgatcaac gaccttccgg gaccagtagc gtcgctcgca tcatcgacgg atgtaagttg
      781 taacgatgga caggatggcg ccgcaagcgt cacgatcagc ggcggcagtt caccgattac
      841 gtatcaatgg ttacccaccg gtgggtcttc ttccagcgct acgggcttgt cggcaggaca
      901 atattccgtc atcatcaccg atgcaaacaa ttgcgttcag accgttggcc tgacgatctc
      961 cgaaccggcg ctgcttactt ctgccgccgc cgccagccag atggtttcgt gttatggctt
     1021 gtccgatgga gcggcttcag tcaatgtgac cggagggacc gcgccgtatc gttacttgtg
     1081 gtcaccctgc gggtccacga ccagtcagat caccaattcc actgccggca actattcggt
     1141 tgtagttacg gacgcgatgg gttgcaccac gacggccgcc actaccatta cggaacccag
     1201 tctgctcact gccagcacca gcgctacaca atccacctgc ggtaacgccg acggaagcgc
     1261 gagcgtttcg gcaaacggag gatcgggagg atatgcttac ctctggcaac ccgggaatag
     1321 tacgcaaccc actgccggcg gtcttgccgc cggtgcatat accgtgacca tcacggatgc
     1381 aaacggatgt acgaccaccg ccattgccgc cgtttccgac atgggcgctc cggtcattca
     1441 gcccggcccg atcattccgg tcaattgtcc cggcggtagc gatggttcca cttctgtgac
     1501 ccttacgggc gggaccgctc ccttccagta tcaatggtcc ggaggagttg ccagcggcaa
     1561 cgctgtgagc ggactgagcg ccggcgctta ttcaatagtt gtgacggacg gcaaccagtg
     1621 cgtcgccgtt caaagctttg tcatcacaga gcctccgccc atcgaacttt catcgctctc
     1681 cctgccggtt tcgtgtcatg gcggaaatga cggagccttg caactgacca ccggcggagg
     1741 aactcccggc tactcctacc tgtggtcgaa cggagcaacc agccaaaccg cgacgatgct
     1801 caccaccgga aattatagcg tcgtggtgac cgacgcgcac ggctgtacgt ccagcctgag
     1861 caatgtgatc actgaacccg atgcgattac agccagcaca agcagcgtcc ctgtactttg
     1921 caacggcgga gtcgacggcg ccgccgagat cgccgtcaac ggcggaaccc caccgtataa
     1981 ttatgaatgg gcaggtatcg gcgtgcagca gccgctg
//
LOCUS       DAOTON010000553         2012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_43572_length_2012_cov_3.118549, whole genome shotgun sequence.
ACCESSION   DAOTON010000553 DAOTON010000000
VERSION     DAOTON010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43572_length_2012_cov_3.118549"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="P5292_10280"
     CDS             complement(<1..486)
                     /locus_tag="P5292_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39555.1"
                     /translation="MNRSLPRFLYYLLTATLLQSIVFAQSPVERAMPDPLRLLQDTAS
                     ITGGSMATDESFREDNAKVLRDRLQQAERRGNLRVSGMLHVRYAGLFLRTGAVDSAVF
                     HFQTAVKAFSTAGMPRGEALAQTGIGLTRERTGDVTGARAAYTAALERAVYQLEIYRA
                     GE"
     gene            606..962
                     /locus_tag="P5292_10285"
     CDS             606..962
                     /locus_tag="P5292_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39556.1"
                     /translation="MLRPALHTLRFVALLAFLMAFLPTGSKAYGRSVLVKATVSEAQA
                     LTTQDQIPTGKGLRVGRLVLSAPVMLLPVKQLPAGWVAVFLPSFGNFSGNVVSLPPYT
                     IVRASHRCTVLQVFRC"
     gene            1269..>2012
                     /locus_tag="P5292_10290"
     CDS             1269..>2012
                     /locus_tag="P5292_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRS39557.1"
                     /translation="MSIYVLIQQSLHRMRPFHMPRSRVQRRKVIRQSLRSFVLISLGA
                     LSAFVGLKGFLLPNHLIDGGATGISILTAYGTPLPLPLLILMINIPFIFMGWRQVGLG
                     FALRASGGIILLSILLSLLTLPTFTSDRLLIAVFGGFFLGAGIGLSIRGGGVIDGTEV
                     LALYISRKTGLTIGDFILVINILIFLTAAWLINLESAMYSTLTYLIASRTVDFLVHGL
                     EEYTGVTIISDRSEEIRKALIYQVGHGVTI"
BASE COUNT          392 a          577 c          555 g          488 t
ORIGIN      
        1 ttcgccagcg cgataaattt ctagttgata gaccgcgcgc tccaatgcgg ccgtataagc
       61 agctcgcgcg cccgtaacat cgccggtacg ctcgcgcgtc agaccgatgc ctgtctgcgc
      121 caacgcttcc ccacgcggca tgccggcggt ggaaaatgcc ttgactgcag tttgaaaatg
      181 gaacacggcc gagtcaacgg cgccggtgcg cagaaacaag cctgcgtacc gaacgtggag
      241 catgccggag acgcggagat taccgcgccg ctcggcctgt tggaggcgat cgcgcaggac
      301 tttcgcgttg tcttcccgaa acgattcgtc cgtcgccatc gagcctccgg tgatggaagc
      361 cgtgtcctgc agtaagcgca acgggtccgg catggcgcgt tccaccggag attgcgcgaa
      421 cacgatcgac tgcaacaagg tggcagtaag caggtagtat aaaaagcgcg ggagagagcg
      481 gttcacgccg ggtcaaatgc gggtgaacga aagtgcgtat tttctgctct gaaggcaagt
      541 gttgtaggat atctactacg attcttccgt caaattgagt atctttgcaa aacaggtctt
      601 cagccatgtt gcgtccggct ctccatacac ttcgattcgt cgccttgctg gcgtttctga
      661 tggcattctt gccgacaggg agcaaggcat atggcagatc cgtgttggtc aaggcgaccg
      721 tatcggaagc tcaggcgctc accacccagg accagattcc aacaggaaaa ggtctgcgcg
      781 tcggtcgcct ggtactatcc gccccggtca tgctgttgcc ggtcaagcaa ctgccggcag
      841 ggtgggtagc ggtgttttta ccctccttcg gtaacttttc gggaaatgtg gtttccctgc
      901 ccccctatac cattgttcgt gcctcgcatc gctgtacggt gttacaggtt tttcgctgtt
      961 gaatcggcct tttgctgacc ggtaattccg gttcccgacc gaaagaacag ttctaactaa
     1021 ttatttccta cgatgtccga atccactaaa cgtggcctcg gatggctctt cccgacctta
     1081 ttcagcgtcg gagtcattct ggccctggta ttactgaaaa tgttgctttg acattgaaga
     1141 gatggcaccg ctctggagag tcggttgcct ggtattaagc ggttccgtgc gcaggtcgca
     1201 ttcatgtggt gatggttgtc ctgacctgcc gcgggccgct ttatctttga ctataaatcc
     1261 gccaagccat gtccatctat gttctcatcc agcagtcgct gcacaggatg cgcccctttc
     1321 atatgcctcg ctcacgcgtc cagcggagga aagtaattcg ccaaagtctg cgcagcttcg
     1381 tcctgatctc cctgggggcc ttgtcggcct tcgtcggcct gaaaggattt ttactgccca
     1441 atcatctgat cgatggcgga gcaaccggga tctcgatcct gacggcctac ggtacgccgc
     1501 tgccgctgcc gctgctgatc ctgatgatca acattccgtt catcttcatg ggttggcgac
     1561 aagtaggcct gggattcgcg cttcgcgcca gcggagggat catcttattg tcgatcctgt
     1621 tgtctctgct cacgctgccg accttcacca gtgatcgcct gttgattgcc gtgttcggtg
     1681 gcttcttcct tggcgccgga atcgggctga gtatccgagg tggcggcgtc atcgacggaa
     1741 cagaagtact ggcactctac atcagccgca agaccggcct aaccatcggc gacttcatcc
     1801 tggtcatcaa catcctgatc ttcctgacgg ccgcgtggct gatcaacctc gagtcagcca
     1861 tgtacagtac cctgacgtac ctgatcgcct cccgtacggt cgacttcctt gttcatggtc
     1921 tcgaagaata tactggtgtg accatcatct cggaccgaag tgaagagatc cgcaaggcgc
     1981 tcatttatca ggtcggacat ggcgttacca tc
//
LOCUS       DAOTON010000554         2011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_43607_length_2011_cov_3.073108, whole genome shotgun sequence.
ACCESSION   DAOTON010000554 DAOTON010000000
VERSION     DAOTON010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43607_length_2011_cov_3.073108"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1362)
                     /locus_tag="P5292_10295"
     CDS             complement(145..1362)
                     /locus_tag="P5292_10295"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRS39558.1"
                     /translation="MKTVDSFNFAGLRVLVRVDFNVPLDKSFRITDDTRIRAALPTIN
                     KILADGGSVVLLSHLGRPLKKLKEDGSIDREKFTLRHAVQRTAELLGRPVRFSDDCIG
                     NDAYAQSAALKPGEVLLLENLRFYKQEEKGDAEFAAQLARHGNFYVNDAFGTAHRAHA
                     STAVIASSFPGKKCFGYLMAAELDNAEKVMHQPARPYTAVMGGAKVSDKILIIENLLD
                     KADNILIGGGMAYTFFKAMGGQVGNSLVEEDKLDLAKSLIEKAKAKGCRLLLPADSVL
                     GDKFDKDANTSIAANNAIPDGWMGLDIGPQAVADFQQVIRESKTILWNGPMGVFEMEK
                     FANGTRKVAEAIAEATVKGAYSLIGGGDSAAAVNQFGFGDKVSYVSTGGGALLEYFEG
                     KVLPGVAAVLSEQ"
     gene            complement(1416..>2011)
                     /locus_tag="P5292_10300"
     CDS             complement(1416..>2011)
                     /locus_tag="P5292_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS39559.1"
                     /translation="VVAVEPSASMRAQRLSAGRVPAIDARADQLPFDDNAFDASMSLL
                     SVHHWPDLPAGLREMRRVTRGPVVIMSYDPDGLDDFWNAHYFPELVAVERARYPKLDT
                     IAAALGGAAEIIRVPIPIDCVDGFQEAFYARPEAFLRAEVRKAQSAWGFLPAGQEAVL
                     VERLATALQNGEWEHRFGHYRRSPFFDGALRLVVACQ"
BASE COUNT          376 a          609 c          618 g          408 t
ORIGIN      
        1 cctacggggt catcgttatc tgatcgactg gcgttgcgtt aatccgcccg cccaggcatg
       61 ctacaggcaa gggcaaactg cctactgttt actgtttact gttcactatt cgctattggc
      121 tattcgctat tcgctccaca ctgttcactg ttcactcaaa acagctgcta ctcccggcag
      181 cacctttccc tcaaaatact ccagcagtgc tccaccgccg gtggaaacat aggagacttt
      241 gtcgccgaaa ccgaattgat tgacggccgc ggcggagtcg cctccgccga tgagggagta
      301 cgcgcccttg accgtcgctt ccgcgatggc ttccgcgacc ttgcgggtac cgttggcgaa
      361 cttttccatt tcgaacacgc ccatcgggcc gttccagagg atggtcttcg attcgcggat
      421 cacctgctgg aagtcggcca cggcctgcgg gccgatgtcg agtcccatcc agccgtcggg
      481 gatcgcgttg ttggccgcga tggaggtgtt ggcgtccttg tcgaacttgt cgccgagcac
      541 cgagtccgct ggcaggagca gtcggcagcc tttcgccttt gccttttcga tcagtgattt
      601 ggccaggtca agcttgtctt cttccaccag cgagttgccg acctgtccgc ccatggcttt
      661 gaagaacgta tacgccatcc cgccgccgat gaggatgttg tcggctttgt cgaggaggtt
      721 ttcgatgatg aggatcttat cgctcacctt cgctccgccc atcaccgcgg tatacggacg
      781 ggcgggctgg tgcatgacct tctcggcatt gtcgagctcg gcggccatga ggtagccgaa
      841 gcacttcttg ccggggaagc tggaggcgat gacggcagtg gaggcatgcg cacggtgcgc
      901 ggtgccgaag gcgtcgttca cgtagaagtt gccgtgccgc gcgagttgcg cggcgaattc
      961 cgcgtcgcct ttctcttcct gcttgtagaa gcgcaggttc tccagcagca gcacttcacc
     1021 cggcttcagg gcagcggatt gagcgtaggc gtcgttgccg atgcaatcgt cggaaaagcg
     1081 gaccggccga ccgagcagtt cggcggtgcg ttgtacggca tgccgcagcg tgaacttctc
     1141 acgatcgatg gatccatctt ctttcagctt cttgagcggg cggcccaggt gggacagcag
     1201 gaccaccgat ccgccgtcgg cgaggatctt gttgatggtc ggcagggcag cccggatacg
     1261 ggtatcgtcg gtgatccgga aagacttatc caggggaaca ttgaaatcca cacgcaccag
     1321 cacccgcagg ccggcgaagt tgaacgaatc gacggttttc atgggtgcga aggtaaaaaa
     1381 cgtgaaaggc acctgaccgc tccgtgtcaa gttgattact gacacgcaac caccaaccgc
     1441 aacgccccat caaagaacgg cgatctgcgg tagtgcccga accggtgttc ccattcgccg
     1501 ttttggaggg cggtggcgag gcgctcgacc agcacggctt cctgcccggc cggcaggaaa
     1561 ccccaggccg attgcgcctt gcggacttcc gcccgcagaa aggcttcggg ccgggcgtag
     1621 aaggcttcct ggaatccgtc gacgcaatcg atcggaatcg gcacgcggat gatctcggct
     1681 gcgccgccca atgccgctgc gatcgtatcg agcttcggat aacgcgcacg ctctacggca
     1741 acgagttcgg ggaagtagtg ggcgttccag aaatcgtcca gcccgtcggg atcgtaggac
     1801 atgatcacca ccggaccgcg cgtcaccctc cgcatctccc gcaaaccggc gggaaggtcg
     1861 ggccagtggt gcacgctgag caacgacatg gaagcatcga acgcgttatc gtcgaacgga
     1921 agctgatccg cgcgtgcgtc gatggcggga acgcgaccgg cagacaggcg ttgcgcacgc
     1981 atgctggccg agggttctac cgcaaccaca t
//
LOCUS       DAOTON010000555         2010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_43629_length_2010_cov_3.955499, whole genome shotgun sequence.
ACCESSION   DAOTON010000555 DAOTON010000000
VERSION     DAOTON010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43629_length_2010_cov_3.955499"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="P5292_10305"
     CDS             complement(<1..1171)
                     /locus_tag="P5292_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRS39560.1"
                     /translation="MSLLKKLASQTAVYGLSSILGRLLNYLLVPLYTRVFDTAEYGVV
                     TQLYAYVTFFNVLFTYGLETAFFRFSQSTGDNKKVYSTGLLSLLLTSVLGAVTIILAS
                     GWLSGELSEGTRTEDYPLYISCFAAVLAFDAIATLPFARLRLQNRATRFVTIRLISIL
                     LNVGLNCFFLLYCPYAIRQDPNSFCAALYDPSFGVGYVFVINVVTSGITLLLLLPELL
                     PVRWGFDPALWKNMLRYGFPLMIAGFAGMINETIDRILLPELVRDPSTSMVQLGIYGA
                     CYKLSILMTLFVQTFRYAAEPFFFTQAGKEDAPKLYATVMHYFVLAGALIFLGVQLYM
                     DWIQEFIGPDFRSGLVVVPILLIANLCLGVYLNLSFWYKITGHTRWGAWFSVFGAV"
     gene            complement(1438..1707)
                     /locus_tag="P5292_10310"
     CDS             complement(1438..1707)
                     /locus_tag="P5292_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRS39561.1"
                     /translation="MEAVHLLIQGRVQGVFFRKFTHLKALELNIRGWVRNLPDGRVEV
                     VAEGDPERLQRFIHWCHEGPEAARVDEVEIHPTPLKNFREFVIVR"
     gene            1793..>2010
                     /locus_tag="P5292_10315"
     CDS             1793..>2010
                     /locus_tag="P5292_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39562.1"
                     /translation="MRLRLLCFLLGFSLFANSSRGQSTRGTELWLTFGENLDLLFNGM
                     PNFSVIVSSPVNTQGAVSIPFTGFSVPF"
BASE COUNT          489 a          536 c          552 g          433 t
ORIGIN      
        1 tgaccgcgcc aaaaaccgag aaccaggcac cccatcgggt atgtccggtg atcttgtacc
       61 agaacgacag gttgaggtaa acgccgaggc agaggttggc gatcaacaga atcgggacaa
      121 cgacgagccc ggatcggaaa tcggggccta tgaactcctg gatccagtcc atgtacaatt
      181 gtacaccgag gaagatcagc gccccggcca gtacaaagta gtgcatgacg gtcgcataga
      241 gtttcggcgc gtcttccttg ccggcctgcg tgaagaaaaa cggctcggcc gcgtagcgga
      301 acgtctgaac gaacagggtc atgaggatgg acagcttgta gcaggcacca taaatcccca
      361 gttgcaccat ggacgtcgaa gggtcgcgca cgagttccgg taacaggatc cggtcgatgg
      421 tttcgttgat catcccggcg aaaccggcga tcatcaacgg gaaaccatag cgcagcatgt
      481 ttttccagag cgccgggtcg aagccccaac gtacaggtag caactccggc aacaagagta
      541 gtaaggtgat gccgctcgtc accacgttga tcacgaagac ataccccacc ccgaacgaag
      601 gatcatagag cgctgcgcag aaactgttcg ggtcctgcct gatcgcgtag gggcagtaaa
      661 gcaggaagaa gcaattcaat ccgacgttca gcaggatgct aatcagccgg atggtcacaa
      721 agcgcgtggc gcgattctga aggcgtaatc gtgcaaacgg cagggtggcg atggcatcga
      781 aggcgagcac agccgcgaag caggaaatgt agagcggata atcctccgtg cgtgttcctt
      841 cgctcagttc accggacaac cagccggagg cgaggataat cgttactgca cccagtaccg
      901 aagtcaacag cagggataag agtccggttg agtagacctt tttgttatca ccggttgatt
      961 gggaaaaccg gaagaaggcg gtctcgagtc cgtacgtaaa gagtacattg aaaaatgtca
     1021 catacgcgta caactgcgtc accaccccat actccgccgt gtcaaatacc cgtgtgtaca
     1081 agggtaccag caggtagttc agcagacgcc cgaggatgct gctcaaaccg taaacggcgg
     1141 tttgggaggc gagctttttg aggagagaca aggtgggttt agggttccga gtttcctgcc
     1201 ttcgctaaag cttcggcagg caaacgggtt tcgggtttcg agtttcaggt ttggagcgtg
     1261 ttatggttcc atccgggata aacaatacac tgcctccatt tgaacagcat ctgcttggtg
     1321 cgctgtcccg gtcaggagtt cacaatcagg cggtagttcc gtacgcccaa accgattccg
     1381 atagtcgttt caggatcagt gctaacgaaa accaggtggt caaatttcct tcaatgctca
     1441 tctcacaatg acgaactcgc ggaaattttt caagggggtg gggtggattt ctacttcgtc
     1501 aacgcgcgcg gcctccggtc cttcatgaca ccagtggatg aatcgctgga gtcgttcggg
     1561 gtccccttcg gccacgacct ctacccggcc gtcaggaagg ttgcgcaccc aaccccgaat
     1621 attgagctcc agggccttca gatgcgtgaa ttttcggaaa aacacaccct gtacccttcc
     1681 ctgaatcagc aaatgcaccg cctccatcag ctgctaaagt taaagcggct gctgaaattc
     1741 cggttaaatc atccttttta taacttgaag ggctcagttc aaccttattc tcatgcgttt
     1801 acgactactc tgcttcttac tcggcttcag cttgtttgcc aattcttccc gtggacaatc
     1861 cacccgggga acggaattgt ggctcacatt cggcgaaaac ctggatctcc tgttcaatgg
     1921 catgccgaat ttttcggtga tcgtcagcag cccggtgaac acgcagggcg ccgtgtcgat
     1981 tcctttcacc ggattctcgg tgccctttat
//
LOCUS       DAOTON010000556         2009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_43686_length_2009_cov_2.256909, whole genome shotgun sequence.
ACCESSION   DAOTON010000556 DAOTON010000000
VERSION     DAOTON010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43686_length_2009_cov_2.256909"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="P5292_10320"
     CDS             <1..324
                     /locus_tag="P5292_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRS39563.1"
                     /translation="KNLWNFTRFYHHESCGQCSPCREGTGWMEKILHRILDGHGDMSD
                     IELLWDIQGKIEGKTICPLGDAAAWPVASAIRHFRKEFEEYVQNPARIKDVRHYYRVN
                     QLTEA"
     gene            397..1398
                     /locus_tag="P5292_10325"
     CDS             397..1398
                     /locus_tag="P5292_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRS39564.1"
                     /translation="MAKVTIDGHLIEVPDGTTILQAARMIGGEIVPPAMCYYSKLKTS
                     GGYCRTCIVKVTKGSEKDPRPMPKPVASCRTTVMDGMEVQNITAPDLMAARAGVVEFL
                     LINHPLDCPICDQAGECHLQDLAYEHGLAKTRYKFARRTFEKIDIGSKIKLHMTRCIL
                     CYRCVKVADQLTDGRVHGVINRGDVAEISTYIQNAVHNDFSGNMIDVCPVGALTDRTF
                     RFVSRVWNVNPFDAHRNCSKCCGRVTLWMKGSEVARVTVRKDRYGEVMDTPDGKAGWI
                     CNDCRFENKQTSDWTIEGPTKLDRHSVISQNHYTLKEPQHKPLLNAVDVVPDSHRLN"
     gene            1430..>2009
                     /locus_tag="P5292_10330"
     CDS             1430..>2009
                     /locus_tag="P5292_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRS39565.1"
                     /translation="MLTSQLIFTIILIVAVFSISLVIAMYSTYAERKVAAFMQDRIGP
                     NRAGPFGILQPLADGVKLFMKEEIIPTVSNRWLFILGPGVAMTTALMTSAVLPWGKGL
                     TIGGEFYAMQVTDVNIGILYMFGVVSIGVYGIMIGGWASNNKYSLMGALRASSQMISY
                     EIAMGLAIIALIMMTGTLSVREIAEQQAGAHWN"
BASE COUNT          453 a          609 c          565 g          382 t
ORIGIN      
        1 aagaacctct ggaacttcac gcgcttctac caccacgagt cgtgcggaca atgcagtccg
       61 tgccgcgaag gcaccggttg gatggaaaag atcctgcacc gcatcctcga cgggcacggc
      121 gacatgagcg acatcgaact gctgtgggac atccagggca agatcgaagg gaagaccatc
      181 tgtccgctcg gcgacgcggc cgcctggccg gtcgccagcg cgatccggca tttccgcaag
      241 gagttcgaag aatacgtaca gaatcccgca cgtatcaaag acgtgcgaca ttattaccgt
      301 gtgaaccaac tgaccgaggc ctgaggcccg gttcggtcca tataccatcg tcctgcgtgt
      361 gctccggcac gaacggggac gtattgaacg aaacgaatgg caaaagtgac catcgacggc
      421 cacctgatcg aagtacccga cggtaccacg atcctccagg cggcgcgcat gatcggcggc
      481 gaaatcgtac caccggccat gtgttattat tccaagctga agaccagtgg cggctattgc
      541 cggacctgca tcgtgaaagt cacgaaaggt tccgaaaagg atccacggcc gatgccgaag
      601 ccggtagcct cctgccgcac cacggtgatg gacggcatgg aagtccagaa catcacagcg
      661 cccgacctga tggccgcccg cgccggtgta gtggagttcc tgctcatcaa ccatccgctg
      721 gattgtccga tctgcgacca ggccggtgaa tgtcacctgc aggatctggc ctacgaacac
      781 ggcctggcca agacccgcta caagttcgcc cgacgcacgt tcgaaaagat cgacatcggc
      841 agcaagatca agctgcacat gacccgttgc atcctctgtt accgttgtgt gaaagtggcc
      901 gaccaactga ccgacggccg cgtacacggg gtcatcaacc gcggcgatgt tgccgagatc
      961 agcacctaca tccaaaacgc cgtccacaac gatttttccg gcaacatgat cgatgtttgt
     1021 ccggtaggcg ccctgaccga ccgcacgttc cgtttcgtca gccgggtctg gaatgtgaat
     1081 ccgttcgatg cccaccgcaa ctgcagcaag tgttgcggcc gcgtgaccct gtggatgaaa
     1141 ggcagcgaag tggcgcgtgt tacggtgcgc aaagaccgat acggcgaagt gatggatacc
     1201 cctgacggaa aagccggttg gatctgcaac gactgccgtt tcgagaacaa gcagacatcc
     1261 gactggacga tcgaaggtcc gacgaagctc gaccgccatt cggtgatctc tcaaaatcat
     1321 tatacgctga aagagccgca acacaaaccg ttgctgaatg ccgtggacgt agtccccgac
     1381 agccaccggc tcaactaagc tccattatca cctccgaccc tatcgtccta tgctgacgtc
     1441 ccaattgatc ttcaccatca tcctgatcgt agcggtcttc tcgatttcgc tggtgatcgc
     1501 gatgtattcc acctatgccg aacgaaaagt agcggccttc atgcaggacc gtatcggtcc
     1561 caaccgggct ggtccgttcg gcatcctgca accgctggcc gacggcgtga agctgttcat
     1621 gaaggaagag atcattccaa ccgtatcgaa ccgatggctg ttcatcctcg gcccgggcgt
     1681 cgcgatgacg accgctttga tgaccagcgc cgttcttccc tggggcaagg ggttgacgat
     1741 cggcggcgaa ttctacgcca tgcaggtcac ggatgtgaac atcgggatcc tgtacatgtt
     1801 cggggtggtt tccatcgggg tttacggcat catgatcggc ggctgggcgt cgaacaacaa
     1861 atactcactc atgggcgcct tacgcgcctc gtcccagatg atctcctacg agatcgcgat
     1921 gggtctggcg atcatcgccc tcatcatgat gaccggcacc ctgagcgtcc gcgagatcgc
     1981 cgagcaacag gcaggcgctc actggaatg
//
LOCUS       DAOTON010000557         2002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_43920_length_2002_cov_2.306112, whole genome shotgun sequence.
ACCESSION   DAOTON010000557 DAOTON010000000
VERSION     DAOTON010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43920_length_2002_cov_2.306112"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="P5292_10335"
     CDS             complement(<1..186)
                     /locus_tag="P5292_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS39566.1"
                     /translation="MTTPVDSSRGARINPKSKYLKSELVFEHPEGLDEAFDPLHPTTY
                     LLEYPKKIINRIDSPDIP"
     gene            complement(291..851)
                     /locus_tag="P5292_10340"
     CDS             complement(291..851)
                     /locus_tag="P5292_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39567.1"
                     /translation="MNIQKTMRKAMILSALLMAGTLVQGQHVVTMKSGEKMSGKVESI
                     QNETLEFLYKGNKMNFPLADLYSINFAEQSSLTAGESTSSAPREVGEKQVTSGSYLVR
                     YKVADRIVAKPPRIDNLTQEKGTVVVDVTIDKYGHVMKAIPGAPGSTTNSEYLKTKAK
                     QAAESALFNNVPTAPLEQKGYMIISF"
     gene            1025..>2002
                     /locus_tag="P5292_10345"
     CDS             1025..>2002
                     /locus_tag="P5292_10345"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRS39568.1"
                     /translation="MIKRLLLTSMLLGGLAASAQTWTMQNVGFISAGAYPFDISVPDA
                     NSAWCVAYDGSGAAANLQEFSRTIDGGATWTAGLVSTGTNLAFSCISAIDADTAYAMM
                     YDADAGAGGGIFKTTDGGANWAQIAVGLIFDGNSFPNVVHFFDAQNGFAMGDPNGGYF
                     EIYTTNDFGASWNRVPSGSIPAPLAGEYGIVNDFDVVGQTIWFGTNKGRVFKSDDGGL
                     NWTAASVPASASRTISAIGFRDANTGICISTPTSGTATVYTSTDGGATWTLLSPAGPM
                     FLSEVDNIPGTNVWVSCGASTVGRGSSYSTDDGASWVGIDTSGQGTMDGY"
BASE COUNT          413 a          569 c          549 g          471 t
ORIGIN      
        1 tgggatatcc gggctgtcga tccggttgat gatcttcttc gggtattcta aaagatacgt
       61 tgtaggatgc aggggatcga aggcctcgtc cagaccttca ggatgctcga atacgagctc
      121 cgacttcagg tacttgctct tcgggttgat ccgcgcgccg cgactgctat ctacgggggt
      181 tgtcatacga tccaaactgc tttctataag aaaagaaaaa agggatcgcg caacacaaga
      241 gccataaaaa aagccatccg aagatggctt ttggtagact ccgcttcgcc ttagaaagaa
      301 atgatcatgt atcccttctg ttctaaggga gcggtgggta cgttgttgaa cagcgcggac
      361 tccgcggcct gcttggcctt tgtcttcagg tattcactat tcgtggtgct tccgggagca
      421 cccggaatgg ccttcatgac gtggccgtat ttatcgatgg taacatccac cacgaccgtt
      481 cctttttcct gcgtcaggtt gtcgatcctt gggggcttag caacgattcg gtcggcaacc
      541 ttatagcgga ccaggtagga tccggaagta acctgcttct cgcctacttc ccggggagcg
      601 gatgatgtcg attcgcccgc ggtcagcgac gactgttccg caaagttgat ggagtacagg
      661 tccgccaacg ggaagttcat tttgtttccc ttgtagagga actcgagggt ctcgttttga
      721 atggactcca ccttaccgct cattttttcg ccggatttca tcgtcacgac gtgctggccc
      781 tggaccaggg ttcccgccat caggagggcg gagaggatca ttgcttttcg catggttttc
      841 tggatattca tgccggagcg gttacaataa ccgggcccgg agctgaccgg gtaaaagtag
      901 ggctcagttt acccgcttcc cggcaacctg ccgggctgtt tttaacagag aagtctacag
      961 ccggggaacc ccgcccggtg catataaatc cctatttttg acccaatcac ttaaacacaa
     1021 ctccatgatc aaacgtctac tccttaccag catgttactt ggaggtctgg ccgcttccgc
     1081 tcagacctgg acgatgcaga atgtcggatt catctccgcc ggcgcatatc ctttcgacat
     1141 ttccgtaccg gatgcgaact ccgcgtggtg cgttgcctat gacggttccg gcgcggctgc
     1201 caacctgcag gaattctccc gcaccatcga tggcggtgct acctggacag ccggcctggt
     1261 ctcgaccggt accaacctcg ccttctcctg tatcagcgcg atcgatgccg atacggccta
     1321 tgccatgatg tacgatgccg acgccggtgc aggcggtggc attttcaaga ccaccgatgg
     1381 cggtgcaaac tgggcacaga tcgccgtagg tctgatcttt gacggcaact cattcccgaa
     1441 cgtggtacat ttcttcgacg ctcagaacgg ttttgccatg ggcgacccga acggcggtta
     1501 cttcgagatc tacaccacga acgacttcgg cgcaagctgg aaccgggtgc cttcaggcag
     1561 tatccccgca ccgctcgccg gtgaatacgg aatcgtaaac gactttgacg tagtcggaca
     1621 gaccatctgg tttggcacca acaaagggcg cgttttcaaa tccgacgacg gcggcttgaa
     1681 ctggaccgca gcgagtgtac ctgccagtgc ttcccgcacg atttcggcca tcggtttccg
     1741 cgacgcgaac accggcattt gcatcagcac tccgaccagt ggtacggcca cggtctatac
     1801 ctcaacggat ggcggcgcaa cctggaccct cctgagccct gccggcccga tgttcctcag
     1861 tgaagttgac aatattcccg gcacgaacgt ttgggtaagc tgcggtgcga gtacagttgg
     1921 tcgcggctct tcctactcaa ccgatgacgg cgccagctgg gttggaatcg acacctccgg
     1981 acagggcacc atggatggtt at
//
LOCUS       DAOTON010000558         1999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_44004_length_1999_cov_2.609568, whole genome shotgun sequence.
ACCESSION   DAOTON010000558 DAOTON010000000
VERSION     DAOTON010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44004_length_1999_cov_2.609568"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="P5292_10350"
     CDS             <1..662
                     /locus_tag="P5292_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39569.1"
                     /translation="LSSANMNNWEYVPGQTYNMTLTVARSGSPLFGLGLEALDASNAN
                     AGTLTITDAASTQIKSKLVGSVTRRNVVHQLDGGATPNSKDFNFAWTAPATDIGPVTF
                     YFSGVASNADGDETIGDYVYDGVQVINPASTTGIGKTDTPASVCFPNPASNQISVRVN
                     GTTSVRLINSEGREVRSLQPLETGSQVITLDQLDVLPRGLYFLELQQAGTRQLEKVIL
                     Q"
     gene            722..1081
                     /locus_tag="P5292_10355"
     CDS             722..1081
                     /locus_tag="P5292_10355"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39570.1"
                     /translation="MQNKLLLAAFGLLITSSACRTDPEVPATPEVSFQTQVQVLLSSN
                     CNMSGCHAANGGEFPLTTYEAVMQHVTAGDARNSKLYQSITGRGADRMPPDGRDPLLD
                     EEITTIYVWIQQGAKNN"
     gene            1093..1503
                     /locus_tag="P5292_10360"
     CDS             1093..1503
                     /locus_tag="P5292_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39571.1"
                     /translation="MKKTILAALLIVSGLTACYYDNFEEIHPTIPGGTTCVIPDTVSY
                     ATNIQPIMNASCGTDDNGCHDAANSINLGGNGSLADYAGTVETIADDGITTFMGRIRQ
                     TVAQSKWMPKGGGKIDDCSIDKIQKWIDQGQINN"
     gene            1518..>1999
                     /locus_tag="P5292_10365"
     CDS             1518..>1999
                     /locus_tag="P5292_10365"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRS39572.1"
                     /translation="MKFRLLLFGFLTVAGAGSLHARDMLSALDTGNEKDYSIATFKGT
                     RLINFHTIETTGPRTLDFRISHRFGAINSGFEEAFGIDQTANIRLGLEYSPDGRFMFG
                     IGRSSFQKMVDGFLKFRLLRQTTNNSMPISMTLFAGAYRAGIKSPVINGFELYEKETS
                     R"
BASE COUNT          492 a          583 c          499 g          425 t
ORIGIN      
        1 ttcttagcag cgccaacatg aacaactggg aatatgtccc aggtcagact tataacatga
       61 cgctgacggt cgcccgaagc ggcagcccgt tgttcggact gggcctcgaa gcgctggatg
      121 catccaatgc caatgccggt acactcacga ttacggatgc agcgtccacg cagatcaaat
      181 cgaaacttgt cggctccgtt acccgacgca atgtggtgca tcagctcgat ggcggcgcaa
      241 cgccaaacag caaggatttc aatttcgcgt ggacagctcc ggctaccgac atcggtcccg
      301 ttacattcta cttttccggc gttgcttcca acgccgacgg tgatgaaacc attggcgatt
      361 atgtctatga tggcgtacag gtgatcaacc cggccagcac aactggaatc ggtaagaccg
      421 ataccccggc cagtgtttgc ttccccaatc cggcttccaa ccagatttcg gtccgagtaa
      481 atggcacgac cagtgtacgc ttgatcaatt cagagggccg cgaggtccgt agcttacaac
      541 cgctagaaac gggatcgcag gtgatcaccc tcgatcaact ggatgtcctg ccgcgcggtt
      601 tgtatttcct cgaacttcaa caggctggaa cacgccagct cgaaaaagta atactacaat
      661 gaatgaaagc cccggaatcg ccggggcttt ttcgtaagat cgtcctccta ccaaacccct
      721 tatgcaaaac aaattgcttc tcgctgcctt tggtctcctg attaccagca gcgcttgccg
      781 aaccgatccg gaagttcccg cgactcctga ggtcagtttt cagacccagg tacaggtact
      841 gctgtcttcg aattgcaaca tgtccggctg ccacgcggca aacggcggcg agtttccgtt
      901 gacaacctat gaggcggtca tgcagcacgt aactgccggg gatgcacgga acagcaaact
      961 ctatcaatcc atcaccggcc ggggcgccga tcgcatgccc ccggatggtc gggatccctt
     1021 gttggacgag gagatcacga ccatctatgt atggattcaa caaggcgcga aaaacaacta
     1081 acttcaccac ctatgaaaaa gaccatcctg gcggcgttat tgatcgtttc cggactaacg
     1141 gcatgctatt acgacaactt tgaggagatc catcccacga tacccggtgg gacgacctgt
     1201 gtcattcccg acaccgtatc ctatgcgacc aatattcaac ccatcatgaa tgccagttgc
     1261 ggaacggatg ataacggctg ccatgacgcg gcgaattcga tcaaccttgg cggcaacgga
     1321 agcctggccg actatgccgg caccgtggaa accatcgccg atgatggcat cacgactttc
     1381 atgggtcgca tccggcaaac ggtcgcccag agcaaatgga tgccgaaagg cggcggtaag
     1441 attgacgact gcagcatcga caagatccag aagtggatcg atcagggaca aatcaacaac
     1501 taaccatcct cacacctatg aaattccgac tacttctctt cggctttctg accgttgcag
     1561 gcgccggcag tcttcacgct cgggacatgc ttagcgctct ggataccggc aatgaaaaag
     1621 attattccat cgccaccttc aaaggtaccc ggctcatcaa cttccataca atcgaaacga
     1681 cgggtccgcg cacactcgac tttcgcattt cccaccgctt tggcgcgatc aacagcggtt
     1741 tcgaagaagc attcgggatc gatcagacag cgaacatccg tctcgggctc gaatacagtc
     1801 cggatggacg tttcatgttc ggcatcggcc gcagttcgtt tcagaagatg gtcgacggat
     1861 ttctgaaatt ccgccttctc cgccagacga ccaacaacag catgccgatc tcgatgaccc
     1921 tgtttgccgg tgcttatcgt gcaggcatca agagtcctgt catcaacggc tttgaattgt
     1981 acgagaagga aaccagccg
//
LOCUS       DAOTON010000559         1996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_44088_length_1996_cov_3.143740, whole genome shotgun sequence.
ACCESSION   DAOTON010000559 DAOTON010000000
VERSION     DAOTON010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44088_length_1996_cov_3.143740"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="P5292_10370"
     CDS             <1..478
                     /locus_tag="P5292_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRS39573.1"
                     /translation="PLLLTAAFMGFFHVPLKPSTILIFSIAFGIASDGTMYFLTKYRQ
                     ELRKHGGSISRTVSLAIHETGLSMIYTAVILFSGFFIFSVSSFGGTSALGILISVTLL
                     IAMCSNLIFLPTLLLSLQKLLITRAFLKEPLIQVFDEEEDVELDALEVRKTEEVEP"
     gene            575..1432
                     /gene="rfbA"
                     /locus_tag="P5292_10375"
     CDS             575..1432
                     /gene="rfbA"
                     /locus_tag="P5292_10375"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRS39574.1"
                     /translation="MKGIILAGGSGTRLHPITLAMSKQLMPVYDKPMIYYPLSVLMMA
                     GINEILIISTPHDLPGFQRLLGDGSELGCKFSYAEQAVPNGLAQAFVIGESFIGKDKV
                     ALILGDNIFYGTGLQQLMHSNIDPEGGVVFAYHVSDPERYGVVEFDKQNKAISIEEKP
                     EKPKSNYAVPGLYFYDNEVVKIAKGLQPSARGEYEITDVNKVYLERGHLKVGILDKGT
                     AWLDTGTFPSLMQAAQFVQVIEERQGLKIGCIEEVAYSMGYIDAEQLRKIAAPLVKSG
                     YGKYLLSLL"
     gene            1564..>1996
                     /locus_tag="P5292_10380"
     CDS             1564..>1996
                     /locus_tag="P5292_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS39575.1"
                     /translation="MTRKILITGGAGFIGSSMAERLAQDPANYIVIVDNLLTGSHSKV
                     PKSIHQNVQFIKADVNDQKEISAIFLAYRFDYVFHYAAVVGVKRTLANPVMVLKDIKG
                     INNILSLSKNTGVKRVFYTSSSEVYGEPVEYPQNEHTTPLNS"
BASE COUNT          466 a          542 c          503 g          485 t
ORIGIN      
        1 gcccttgtta ttgaccgccg ctttcatggg attcttccac gttcccttga agccgagcac
       61 gatcctgatc ttcagcatcg cctttggaat cgccagtgac gggaccatgt acttcctcac
      121 caaataccgg caggaattac ggaaacatgg cggcagtatt tcgcgtacgg tctccctggc
      181 catacacgag accgggctga gcatgatcta taccgcggtc atcctcttta gtgggttctt
      241 cattttctcc gtttccagct ttggaggaac ctccgccttg ggcattctca tttccgtcac
      301 cctgttgatc gccatgtgca gcaacctgat cttcctgccg accttgttgt tgtcgctgca
      361 gaagttgctg atcacccgcg cattcctcaa ggagccgctt atccaggtgt tcgacgagga
      421 ggaggatgtc gagttggatg ccctcgaagt tcgtaaaacc gaagaagtag agccgtaatc
      481 gcttccgatc cgttcaaaac ccggaaacga ttccgcgtat cgttccggtc ttttcgacga
      541 ttcccttatt ttcgtacccc attccttccc gaccatgaaa ggcatcatcc tcgccggcgg
      601 atccggcacc cgcttgcatc ccatcaccct ggccatgagc aaacagctga tgccggtcta
      661 cgacaaaccg atgatctatt atccgttgtc ggtgttgatg atggcgggca tcaacgagat
      721 cctgatcatc agtacgccgc acgatctgcc gggctttcag cgcttgttgg gtgacggttc
      781 cgaactcgga tgcaagttct cctatgcaga gcaagccgta ccgaacggac tcgcacaggc
      841 cttcgtgatc ggagagtcgt tcattggaaa ggacaaggtc gcgctgatcc tgggcgataa
      901 catcttttac ggtaccggat tacagcagtt gatgcacagc aacatcgacc cggagggtgg
      961 ggttgttttc gcgtatcatg tgagcgatcc cgaacgttac ggggtagtgg aattcgataa
     1021 gcagaacaaa gccatttcca tcgaggaaaa gccggagaag cccaaatcga attacgcggt
     1081 gcccggactt tacttctatg ataacgaagt cgtgaagatc gcgaagggac ttcaacccag
     1141 tgcgcggggc gagtacgaga tcaccgatgt aaacaaagtg tacctcgagc gcggacacct
     1201 caaggtgggt attctcgaca aaggcacagc ctggttggat accggtacgt tcccctcgct
     1261 catgcaggcg gcgcaatttg tacaggtaat tgaagagcgc cagggcctca agatcggttg
     1321 catcgaagag gtcgcgtaca gcatgggcta catcgacgcc gaacaactaa ggaaaattgc
     1381 cgcaccgctg gtcaagagcg gatacggcaa atatttatta tccctgcttt aacaggcaat
     1441 ttggtcgctg taaacccgtt attcaccccg atttgcggtt aattggtgga aatcttatct
     1501 tcgggttcgg gaatttaagc catttttagc atcctgactc gatttcccgc tacctgcgac
     1561 ctcatgacac gtaaaatcct gattaccggt ggtgccggct tcattggaag cagcatggct
     1621 gagcgcctgg cgcaagatcc ggctaattat atcgtgattg tcgataatct gttgaccgga
     1681 tcacattcga aggtgccgaa atcgattcac cagaatgtcc agttcatcaa ggcggacgtc
     1741 aatgaccaaa aagaaatctc cgccattttc ctggcttacc ggttcgatta cgttttccat
     1801 tacgcggctg ttgttggcgt aaaacgtacc ctggcgaatc cggtcatggt cctcaaggac
     1861 atcaagggga tcaataacat cctcagcctt tcgaagaata ccggtgtaaa acgggtcttc
     1921 tatacctcct catccgaagt gtacggcgaa ccggttgagt atccccagaa cgaacatacc
     1981 acgcccctca attcac
//
LOCUS       DAOTON010000560         1991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_44241_length_1991_cov_3.380165, whole genome shotgun sequence.
ACCESSION   DAOTON010000560 DAOTON010000000
VERSION     DAOTON010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44241_length_1991_cov_3.380165"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..1337)
                     /gene="prfA"
                     /locus_tag="P5292_10385"
     CDS             complement(267..1337)
                     /gene="prfA"
                     /locus_tag="P5292_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRS39576.1"
                     /translation="MLEQLDAIKQRFFDVELAISSPDAVSDRKRFAQLNREYKDLKAL
                     VDKADEYRTVVSNLEHARQVIQNEKDEEFREMARLELDDLSARSTVLEEEIRQLLLPA
                     DPEDAKDAILEIRAGTGGDEASLFAGDLFRMYTRYCERRGLRVETVDFTEGTAGGFKE
                     VILNIHGDGAYGILKYESGVHRVQRVPATETQGRIHTSAATVVVLPEADEFDVDIKAN
                     DIRKDLFCSSGPGGQSVNTTYSAVRLTHLPTGIVAQCQDEKSQIKNYEKALSVLRSRI
                     YEMELNKRLEERAKKRKTMVSTGDRSAKIRTYNYPQSRVTDHRIGMTVYNLQAFVDGD
                     IQDMIDALQVAENAERMKEGRG"
     gene            complement(1406..>1991)
                     /locus_tag="P5292_10390"
     CDS             complement(1406..>1991)
                     /locus_tag="P5292_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRS39577.1"
                     /translation="EYNGGMGSNGLTSARHDVFSKAVAEQFPETYDPELDPNVTYCGS
                     RKLTDPVDIGNGKTMHAAKLVLSPTRTYAPVIRQVLAEMRQDIHGIVHCSGGAQTKVL
                     HFVDGLHVIKDQLFAIPPLFNMIRESSGADWKELYKVFNMGHRMELYCEARHAERILA
                     IATAFQLEAKVIGRCEAATENRLSLTTPEGVLVY"
BASE COUNT          412 a          530 c          536 g          513 t
ORIGIN      
        1 aacaccaaag ataaaaagac acccccaaga ccatagggtc cagggggtat tttttggctg
       61 agaagctgaa tctacggatt gcatatgtct caaaatcaca ttcagccaag ttatcttaga
      121 aaaattacgt ttatgagttg aacacggctt ttaaagttag tttctagttt ctaattatta
      181 attacaaatc actagttacc aacttttctt ccatcttaat ccggctccgt atcatttttc
      241 aattacaaat ctccaatcac caatcttcac ccacgccctt ccttcatccg ctccgcgttc
      301 tctgcaactt gtagcgcgtc gatcatgtcc tggatgtcgc cgtcgacgaa tgcctgaagg
      361 ttataaaccg tcatgccgat ccggtggtcc gtgacccggc tttgcggata attgtacgtg
      421 cggatcttgg cggagcggtc gccggtgctg accatggtct tccgtttttt cgcacgttcc
      481 tccagtcgct tgttcaattc catctcgtag atgcgtgaac gaagcacgga tagcgccttc
      541 tcgtaattct tgatctgtga tttttcgtcc tgacactgcg cgacgatacc ggtcggaagg
      601 tgtgtcagcc ggacggcgga gtaggtggtg ttgaccgatt gcccgcctgg gccggaggag
      661 cagaacaggt ccttgcggat atcgttcgcc ttgatgtcca cgtcgaattc atcggcttcc
      721 ggcaatacga ccaccgtggc ggccgaggtg tggatgcgtc cttgcgtctc cgtcgccggc
      781 acgcgttgga cccggtgcac gccggattcg tatttgagga ttccgtatgc gccatcgcca
      841 tgaatgttca ggatcacttc cttgaatcca cccgctgtgc cttcggtgaa gtcaaccgtt
      901 tcgacgcgca agccacgacg ttcacagtaa cgcgtgtaca tgcggaagag gtcgcccgca
      961 aaaagactgg cttcgtcacc gccggtgccc gcgcgtatct ccaggatggc gtctttggca
     1021 tcttcgggat cggcgggtaa taagagttgt cggatctctt cctccagtac ggtggatcgt
     1081 gcgctgaggt catcaagttc caggcgagcc atctcccgga attcttcgtc cttctcgttc
     1141 tggataactt gtcgtgcatg ttcgagattg ctgacgactg tccggtattc gtccgcttta
     1201 tccaccaacg ctttcaggtc cttgtactcc cggttcaact gggcaaagcg tttacggtcg
     1261 gatacggcat ccggcgaact gatggcaagc tctacgtcga agaagcgttg cttgatagcg
     1321 tcgagttgtt ctaacaacat gaagggtcaa cgggaggtaa ggaagtatgg agaaacctaa
     1381 ggaagcgaaa tcgacggtcc ggtcgctaat acaccaagac gccttccggt gtggtcaggc
     1441 tgaggcgatt ttctgtcgcc gcttcgcatc gcccgatcac tttcgcttcc agttggaagg
     1501 ctgtggcaat ggccaggatg cgttctgcat gtcgggcttc gcaataaagc tccatgcgat
     1561 ggcccatgtt gaagaccttg tacagttcct tccaatcggc tccgctggat tcacggatca
     1621 tgttaaagag cggaggaatg gcgaaaagct ggtctttgat cacatgcaga ccgtcgacaa
     1681 aatgcaacac ctttgtctgt gcaccgccgc tgcaatgcac gatgccgtga atgtcttgtc
     1741 gcatttcggc cagcacctgg cggatgaccg gcgcataggt acgtgtgggt gacaaaacca
     1801 gtttggcagc atgcatcgtc ttgccgttgc cgatatcaac cgggtcggtg agcttgcggc
     1861 tgccgcaata ggttacgttg gggtccaact ccgggtcata agtttccggg aactgttccg
     1921 cgacggcttt ggagaagacg tcgtgtcggg cactggtaag gccgttgctg cccatgccgc
     1981 cgttgtattc a
//
LOCUS       DAOTON010000561         1988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_44339_length_1988_cov_3.013968, whole genome shotgun sequence.
ACCESSION   DAOTON010000561 DAOTON010000000
VERSION     DAOTON010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44339_length_1988_cov_3.013968"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1988)
                     /locus_tag="P5292_10395"
     CDS             complement(<1..>1988)
                     /locus_tag="P5292_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRS39578.1"
                     /translation="GVKILWDVNLLPIGKSIRFGFFGLVWTSTFLGYIFHKSPDLLFL
                     AGGYGYQMSHALNNLFGFIGTGALLVFSMVGFGVVAFNLPFKRLRQEQEELEPVTADN
                     AADILTENVALKNALRQEDAPRTVELELGSEDEEEDAEEEEEEEGEFIDLDEQEEEAE
                     EEVVEEDSLELSLEPVLAGTQVTPPIEGEVPLEVAAPPIEETILETEGELPHAQGDYD
                     PTLDLSSYQYPTLELLENYGDKKIEVNKDELEANKNRIVETLSHYNISIEKIKATIGP
                     TVTLFEIVPQAGIRISKIKNLEDDIALSLSALGIRIIAPIPGKGTIGIEVPNQKAEVV
                     PMKSVLASEKFANSEMELPVALGKNISNEVFVADLTKMPHLLMAGATGQGKSVGLNAI
                     LVSILYKKHPAQVKFVLVDPKKVELTLFKTIERHFLAKLPNEEEAIITDTKKVINTLN
                     SLCIEMDQRYELLKDAQVRTIKEYNAKFIGRKLNPNNGHKFLPYIVLVIDEFADLIMT
                     AGKEVETPIARLAQLARAVGIHLIIATQRPSVNIITGTIKANFPARIAFRVTSKIDSR
                     TILDSGGAEQLIGRGDMLFSTGADLVRLQCAFVDTPEVERITDFIGGQRAYADAHLLP
                     EYVDESAEGSREFDANDRDPLFEEAAHIIVQHQQGSAS"
BASE COUNT          427 a          511 c          530 g          520 t
ORIGIN      
        1 aaagaagcag aaccctgctg atgctggacg ataatatggg ctgcctcttc aaagagcgga
       61 tcccgatcat tcgcgtcaaa ttccctggat ccctcggcgc tttcatccac atattccggt
      121 aggaggtggg catccgcgta ggcgcgttgt ccgccgatga agtccgtgat ccgttcgact
      181 tccggagtat cgacaaaggc acactgcaac cgcacgagat ccgcgccggt tgagaaaagc
      241 atatcgcctc gcccgatgag ctgttcggct ccaccgctgt cgaggatggt cctcgaatcg
      301 attttggacg ttacgcggaa tgcgatgcgt gccgggaagt tcgccttgat ggtacccgtg
      361 atgatgttga cagacgggcg ctgcgttgcg atgatcaggt ggattccgac cgcacgagca
      421 agttgtgcga ggcgggcgat cggtgtttcg acttccttcc cggcagtcat gatcaggtcg
      481 gcgaattcgt cgatcacgag cacgatgtaa gggaggaact tgtgcccgtt attcgggttc
      541 agcttacgcc cgatgaattt ggcgttgtat tctttgatgg tccgtacctg agcatccttg
      601 agcaattcat aacgctggtc catttcaata cataaggaat tgagtgtatt gatgaccttt
      661 ttggtatcgg tgatgatcgc ttcttcctca ttgggaagtt tggcgagaaa atgccgctcg
      721 atcgttttga agagcgtcag ttcgaccttc ttgggatcga ccagtacgaa cttcacctgg
      781 gccgggtgtt tcttatagag aatggatacg aggatcgcgt tcaatccaac cgacttaccc
      841 tgacccgttg cacctgccat cagcaagtgg ggcattttag tcaggtcagc cacgaatact
      901 tcattggaga tgttctttcc gagagcaacc ggaagctcca tttcggagtt ggcgaatttc
      961 tcgcttgcca aaaccgattt catgggcacc acttcggcct tttggttggg tacttcaata
     1021 ccgatggtac ccttgcccgg aatgggggcg atgatccgga tacccagtgc cgaaaggctc
     1081 aacgcgatat catcttccag gttcttgatc tttgaaatac ggatacccgc ttgcgggaca
     1141 atctcgaaaa gcgtgaccgt tggaccgatc gtcgccttga tcttttcgat cgagatattg
     1201 taatgactca gtgtttcgac gatgcggttc ttgttcgctt cgagttcatc cttattgacc
     1261 tcgattttct tgtcgccgta attttccagc agttcaaggg tagggtactg ataggaagaa
     1321 aggtccaggg tagggtcata gtcaccctgc gcatgcggca attcaccttc ggtctcgagt
     1381 attgtttcct cgatgggtgg cgcagcaact tcgaggggga cttctccctc gatcggtgga
     1441 gtcacctggg ttcctgcgag aacgggttca agcgataact ccagtgaatc ttcctcgacc
     1501 acttcttctt cagcttcttc ttcctgctca tcaaggtcga tgaattcacc ttcttcttcc
     1561 tcttcttctt cagcatcctc ctcttcatcc tctgatccca actcaagttc tacggtccgt
     1621 ggcgcgtcct cctgacggag tgcgtttttc agggcaacgt tttctgtcag gatatccgca
     1681 gcattatcgg cggttacagg ctcgagctct tcctgttcct gacgaaggcg tttgaagggt
     1741 aggttgaacg cgacaacacc gaagccgacc atcgagaaga ccaagagtgc accggttccg
     1801 ataaagccga acaggttgtt cagcgcatgg ctcatctggt agccgtatcc accggctaaa
     1861 aagaggaggt cgggcgattt atggaagatg taccctaaaa aagtcgaggt ccagacgagt
     1921 ccaaagaatc caaaccggat ggatttgccg atgggcaaca ggttgacatc ccacaggatt
     1981 ttaacgcc
//
LOCUS       DAOTON010000562         1988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_44350_length_1988_cov_2.135541, whole genome shotgun sequence.
ACCESSION   DAOTON010000562 DAOTON010000000
VERSION     DAOTON010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44350_length_1988_cov_2.135541"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="P5292_10400"
     CDS             <1..1009
                     /locus_tag="P5292_10400"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HRS39579.1"
                     /translation="FGILPPMEVSNKRQDGSPCFTDLRLFCLLLLALQSASVAMAQEK
                     SDSSGIPLLSPWENATRPHPGRTRLIAGTTLGLYPLSMTWLYAQWYKDYPQSGFHFFN
                     DAGEWEQMDKVAHAWDAYSIAKPLARTFRWAGHGTHRSALYGAGIAFLYQTTIEVFDG
                     FSAEWGFSVPDMLANSFGTGAFLSQELLWQEQRITLKYSFHLSEYARFRPEVLGETFP
                     ERILKDYNGMTCWAVLNPASFLKGNEKFPRWLSVAIGYGADGMTGGEENPPAVDGVPI
                     PSFERRRQFYLSVDVELSRIRTRSRFLKAVGSVINLIHLPAPALEISPGRSVKGHWLY
                     F"
     gene            complement(1010..1327)
                     /locus_tag="P5292_10405"
     CDS             complement(1010..1327)
                     /locus_tag="P5292_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRS39580.1"
                     /translation="MKEVTVQELKEKRDNGEDFQLIDVRESYEFEVCNLSGELIPMGT
                     IFEQIDRIARDKDVIIHCKSGGRSGVVVQELERRYGFTNLYNLRGGILAYAREIDHSI
                     PTY"
     gene            1398..1907
                     /locus_tag="P5292_10410"
     CDS             1398..1907
                     /locus_tag="P5292_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRS39581.1"
                     /translation="MDSTTNTEILVYAGFWRRFAAMLIDYLILGALHMIVIVPVLGML
                     GIGAVASGIDAEGMSEDDQAMLFMAILSGAAILYVVLAIIGWLYYALMESSSRQATVG
                     KIALGLRVTDLNGERITFLRATGRYFGKIISGMILYIGFIMAAFTEKRQALHDLMAEC
                     LVVSNPSQS"
BASE COUNT          460 a          501 c          517 g          510 t
ORIGIN      
        1 cttcggtatc ttgccaccga tggaagtgag caacaaaaga caggacgggt caccctgctt
       61 tactgatctt cgtttatttt gcttgctgct gcttgcgctc caatccgctt cggtagcgat
      121 ggctcaggag aagtccgatt catccggcat cccattgctt tctccctggg agaacgcgac
      181 acgaccacat ccagggagaa ccagactgat cgccggtaca accctgggct tgtatccgct
      241 gagcatgacc tggctgtatg ctcaatggta taaagattat cctcagtcag gtttccactt
      301 tttcaacgat gccggtgaat gggaacaaat ggataaagtt gcgcatgcct gggatgcgta
      361 ctcgatcgcc aagccgctcg ccaggacctt tcgatgggca ggacatggta ctcaccgatc
      421 cgcgctctac ggtgcaggta tcgcgttcct ttaccaaacc accattgaag tgttcgacgg
      481 attttccgcc gaatggggat tctccgtacc ggatatgctg gcgaacagct ttggtacagg
      541 tgcattcctt tcgcaagagt tattgtggca ggagcagcgt atcacactga aatattcgtt
      601 tcacctctcc gaatatgcgc ggtttcgacc ggaggtactc ggtgaaacgt ttccggaaag
      661 gatcctgaag gactacaatg gaatgacgtg ctgggcagtc ctaaacccgg caagtttcct
      721 gaaggggaac gaaaaatttc cccgctggtt gagtgtcgca atcggttatg gtgccgacgg
      781 gatgaccgga ggtgaagaaa atcctcctgc cgtggatggc gtaccgattc catccttcga
      841 acgccgaagg caattctatc tcagtgttga tgtggagcta tcgcgcatcc ggacacgatc
      901 acggttcctg aaagcggtgg gaagtgtgat caacctgatc cacttaccgg cccccgccct
      961 ggaaatttca ccgggtagga gtgtgaaggg ccactggtta tacttctgat caatacgtgg
     1021 gaatcgaatg gtcgatctcg cgggcgtagg cgagtatccc accgcgcagg ttgtacaaat
     1081 tggtgaagcc ataccgtcgt tccagttcct ggaccacgac accggaacga ccgccggatt
     1141 tgcaatgaat gatcacgtct ttatcgcggg caatacgatc gatctgttcg aagatggtcc
     1201 ccatcgggat aagctctccg gaaagattgc aaacttcgaa ctcataggat tcccgtacat
     1261 cgatgagttg aaaatcttct ccattatcgc gtttctcctt taattcctgg acggtgactt
     1321 ccttcatgaa cctcttggtt ttgagacagt tcaaaaataa cttaaattgt ccattcacac
     1381 actaacccat ctgctttatg gactctacca cgaatactga aatccttgta tatgcaggct
     1441 tttggcgaag gtttgcagcg atgttgatcg actatttgat actcggcgcc ttgcacatga
     1501 tcgtaatcgt tccggtactt ggaatgttag gcatcggtgc ggttgccagc ggtatcgatg
     1561 cggaaggcat gtcggaagac gatcaggcca tgttgttcat ggctattctc agcggtgcgg
     1621 caatcctgta tgtagtactg gccattatcg gttggttgta ctacgctttg atggagtcca
     1681 gttcccggca agccacggtc ggaaagatcg ctttaggctt aagggttact gatctgaatg
     1741 gcgaaaggat aactttcctg cgcgccaccg gccggtattt tggaaagatc atttccggca
     1801 tgatcctgta cattggtttc atcatggcag ccttcacgga aaagaggcag gccttgcatg
     1861 acctgatggc ggaatgcctc gttgtatcga atccctcaca atcctaaatc cacttacatg
     1921 aaaagaacgc tacgcatcct gagtgcctgt ctattacttg ctcagcaagc attcgcaggc
     1981 agcggcgg
//
LOCUS       DAOTON010000563         1987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_44381_length_1987_cov_2.593168, whole genome shotgun sequence.
ACCESSION   DAOTON010000563 DAOTON010000000
VERSION     DAOTON010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44381_length_1987_cov_2.593168"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /gene="rpsC"
                     /locus_tag="P5292_10415"
     CDS             complement(<1..778)
                     /gene="rpsC"
                     /locus_tag="P5292_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRS39582.1"
                     /translation="MGQKANPIGNRLGIIRGWDSNWFGGKKYADKLVEDEKIRKYLLA
                     RLSKGGVAKIVIERTLKLITVTIHTARPGIVIGKGGQEVDKLKEELKKLTKKDVQINI
                     FEIKRPELDAQLVADSICRQIEARISFRRAAKMAIASTMRMGAEGVRVMVAGRLGGAE
                     IARTEQYKDGRIPLHTFRADIDFAIAEAQTSYGKIGAKVWICKGEIYGKRDLSPNIGS
                     GTSSPAPKGLTATGRDRGDRGGRGGRGGDRGGRGGQGGRGG"
     gene            complement(840..1274)
                     /gene="rplV"
                     /locus_tag="P5292_10420"
     CDS             complement(840..1274)
                     /gene="rplV"
                     /locus_tag="P5292_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRS39583.1"
                     /translation="MGVRKRESADKRKEVMKSTAIARLVNVPTSPRKMRIVADLIRGK
                     EVMQAMNILRYNAKAPAARLEKLLQSAIANWEAKTGDKIDEGTVCVKMVQVDSARMLK
                     RLRPAPQGRGYRVRKRSNHVTIILDRMVAAPAKGKKQQEEAA"
     gene            complement(1301..1579)
                     /gene="rpsS"
                     /locus_tag="P5292_10425"
     CDS             complement(1301..1579)
                     /gene="rpsS"
                     /locus_tag="P5292_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRS39584.1"
                     /translation="MARSLKKGPYIAHYLEQKVTKMNESKKKTVIKTWSRASMIAPEF
                     VGHTFAVHNGNKFIPVYVTENMVGHKLGEFAPTRTYRGHSGNRKDDKK"
     gene            complement(1638..>1987)
                     /gene="rplB"
                     /locus_tag="P5292_10430"
     CDS             complement(1638..>1987)
                     /gene="rplB"
                     /locus_tag="P5292_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRS39585.1"
                     /translation="SFAQLAARDGNYAIIKMPSGETRKVLASCLATIGTVSNPDHNKE
                     SLGKAGRNRWAGRRPRVRGVAMNPVDHPMGGGEGRASGGHPRSRKGLYAKGRKTRRKN
                     HPTNKYIIERRKK"
BASE COUNT          358 a          593 c          533 g          503 t
ORIGIN      
        1 gtccgccacg tccgccttgt ccgcctcggc cgccacggtc accaccgcgt ccaccccgtc
       61 ctccacgatc tccacggtca cgaccggttg cggtcagacc ctttggagcc ggcgaagatg
      121 tgcctgaacc gatgttcgga gagagatcac gcttgccata gatctcgcct ttgcagatcc
      181 acactttcgc gccgatcttt ccatacgagg tctgtgcttc cgcgatagcg aagtcgatat
      241 cggcacggaa ggtgtgcagc ggaatacgac cgtccttgta ctgctcggta cgtgcgattt
      301 cagcaccgcc cagacgaccg gctaccatga cacggacacc ctcggcgccc atgcgcatcg
      361 tcgacgcgat ggccatcttg gctgcgcgac ggaacgagat acgggcctcg atctgacgac
      421 agatgctgtc ggcaaccagc tgcgcatcca gttcaggacg cttgatctca aagatgttga
      481 tctggacgtc cttcttggtg agcttcttca actcttcctt cagtttgtcc acttcctgac
      541 cacctttacc gatcacgata cccggacgtg ccgtgtggat cgttacggtg atgagcttca
      601 gggtacgctc gatcacgatc ttggcaacgc cgcctttgga gaggcgtgca aggaggtatt
      661 tacggatctt ctcgtcctct accagcttgt cagcatactt cttgccgcca aaccagttgg
      721 agtcccaacc gcggatgatc cccagacggt tgccgatcgg atttgccttt tgtcccatga
      781 gttgtttctt cagacgcttc gctgttggaa gcggattatc gtgatgatgg ttttacgaat
      841 taggctgctt cttcctgttg cttcttaccc tttgccggcg ccgcaaccat gcggtccagg
      901 ataatggtca cgtggttcga acgcttgcgt acacggtagc cgcgaccctg cggggccgga
      961 cgaagacgct tcaacatgcg ggcgctgtcc acctgtacca tcttgacgca aacggtaccc
     1021 tcgtcgatct tgtcgccggt cttggcttcc cagtttgcaa tggccgattg caacagcttt
     1081 tccaggcggg ctgcaggcgc cttcgcgtta taacgcagga tgttcatcgc ctgcattact
     1141 tcttttccac ggatcaggtc cgctacgatg cgcatcttgc gcggagacgt cggcacattc
     1201 accaggcggg cgatggccgt gctcttcatc acttctttgc gcttatcagc ggattcacgt
     1261 tttcttactc ccatgacgta tcaggatcgt taatgagggt ttatttcttg tcgtccttac
     1321 ggtttccgct atgaccacgg taggtgcggg tcggagcgaa ttcaccgagt ttgtgaccca
     1381 ccatgttttc cgtcacatac accgggatga acttgttccc gttgtgaacc gcgaaggtgt
     1441 gaccgacgaa ttccggtgcg atcatcgaag cacgtgacca ggtcttgatc accgtcttct
     1501 tcttgctttc attcatcttc gtcaccttct gttcgaggta atgagcgatg taaggacctt
     1561 tcttaagcga acgagccata gtattgctgt gttgttcgcc gcggcttccc gatactcagg
     1621 ctgccgccgg caacatatta tttcttgcgt ctttcgatga tgtatttatt cgtcggatga
     1681 ttcttgcgac gggtcttgcg tcccttcgcg tacaagccct tgcgggagcg cgggtgtcca
     1741 cccgaagcgc ggccttcgcc accacccatg gggtgatcaa ccgggttcat ggccacacca
     1801 cgaacacgcg gacgacgacc cgcccaacgg ttacggccgg ctttacccag gctctctttg
     1861 ttgtggtccg gattcgatac ggtaccgatc gtcgcgaggc aggaagccag taccttacgg
     1921 gtttcgccgg aaggcatctt gatgatggcg taattaccgt cgcgggccgc gagctgggca
     1981 aacgatc
//
LOCUS       DAOTON010000564         1980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_44579_length_1980_cov_3.286753, whole genome shotgun sequence.
ACCESSION   DAOTON010000564 DAOTON010000000
VERSION     DAOTON010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44579_length_1980_cov_3.286753"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="P5292_10435"
     CDS             complement(<1..491)
                     /locus_tag="P5292_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39586.1"
                     /translation="MNLRSRAGRVFRYLFQTFLVLLFAGMNNAVQAQFFWVEDFGDNG
                     GICDSYSFANNYVTANGMWEVALIGVNDTAANDFFISAGEPGKPEGDCSSEGCWSTAI
                     FESNRTLHVGNVTNSPNALIICPAGDCGALYDPGGFQIAVETKKRAQSPVIDCSSQNN
                     VVLT"
     gene            complement(522..1496)
                     /gene="meaB"
                     /locus_tag="P5292_10440"
     CDS             complement(522..1496)
                     /gene="meaB"
                     /locus_tag="P5292_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRS39587.1"
                     /translation="MANDPNLYFVEMQEPGYDAAADLSAIRGGDRKALARAITRVENE
                     LTGYRELLHLLTPETGVPVTGFTGPPGAGKSTLINALVRHLRMAGKTVAVIAVDPTSP
                     FNYGSLLGDRLRMQEHFNDEAVFIRSIATRGSLGGLSEKVIELVDLMRCAGFDHIIVE
                     TVGVGQSEVEIAGLADTTVVVLVPEAGDEIQTLKSGIMEIADVFVVNKADREGAETFA
                     NNLRKLAAHPRPDGWEVPVVKTTANADVGVQEVVDAIERHHTSSSMNAKRPYLLAEKA
                     FRLIRRERMRGLTKAQLKEALEKELHAPRFNLYQFADQWVRRIARNDN"
     gene            1543..>1980
                     /locus_tag="P5292_10445"
     CDS             1543..>1980
                     /locus_tag="P5292_10445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS39588.1"
                     /translation="MIRLSAVIITFNEEKNIGRCLESLKGVADEIVVLDSFSTDQTES
                     ICREHGVRFIRHPFGGYIEQKNEVLRLAHYDHVLSLDADEALSPQLRASILDLKANWQ
                     LDAYSFNRLTWYCRRFMRHGGWYPDTKVRLFVRGKGAWGGENPH"
BASE COUNT          420 a          554 c          543 g          463 t
ORIGIN      
        1 gtcagcacga cgttgttctg gctgctgcaa tcgatgaccg gagattgcgc ccttttcttc
       61 gtttcaacgg cgatctgaaa tccacccgga tcgtagagtg cgccgcaatc acccgcggga
      121 caaatgatca aggcattggg tgaattggtg acgttgccga cgtgtaaggt tcggttggat
      181 tcgaaaatgg ccgtgctcca acaaccttcc gaactgcagt cgccttccgg tttaccgggc
      241 tcacccgcac tgatgaagaa gtcgttggct gctgtgtcgt tgacgccgat cagcgccact
      301 tcccacattc catttgccgt gacgtaattg ttcgcaaacg aataggagtc gcagatgccg
      361 ccgttgtctc cgaaatcctc cacccagaaa aactgtgcct gcacagcgtt gttcatgccg
      421 gcaaaaagca gcaccagaaa ggtctggaag agataacgga aaaccctgcc tgcgcgggag
      481 cgtaggttca tatcaatcgt ggaaagcggt tcaatttaca actaattgtc attccgggca
      541 attcgcctaa cccattgatc ggcaaactga tacaagttaa agcggggggc gtggagctcc
      601 ttttccaaag cttctttcaa ttgggctttg gtcagcccgc gcatccgttc gcggcggatg
      661 agccggaaag ctttttcagc cagcaggtaa gggcgtttgg cattcatgct gctgctggta
      721 tgatgacgtt cgatcgcgtc tacgacttcc tgtacgccca catccgcgtt ggcggttgtt
      781 ttaacgaccg ggacttccca cccatccgga cgcggatgcg cagccagttt gcgcagattg
      841 ttggcaaagg tttccgcgcc ttcgcgatcg gctttgttga ctacgaatac gtccgcgatt
      901 tccatgatgc cggacttaag cgtttggatc tcgtcgcccg cttccggtac aagtaccact
      961 acggtggtgt ccgcaagtcc ggcaatttcc acttcggatt gtccgacgcc gacggtttcc
     1021 acgatgatgt ggtcgaaacc tgcacaacgc atcaggtcaa cgagctcgat gaccttttcc
     1081 gatagcccac cgagggaacc gcgggtagcg atggagcgga tgaagacagc ttcgtcgttg
     1141 aagtgttcct gcatccggag ccggtcaccg agcagcgaac cgtagttgaa tggggaggta
     1201 ggatccaccg caatgaccgc taccgtttta ccggccatac gtaaatggcg caccaatgca
     1261 ttgatcaggg tactcttacc agcacccggc ggtccggtaa atcctgtgac cggcacacct
     1321 gtttccggtg tcagcaaatg gagcagttcc cgatatccgg taagttcatt ttccacccgg
     1381 gtaatggcgc gtgcgagtgc cttacggtct ccgccccgaa tggcggacag gtcggcagcg
     1441 gcatcgtatc cgggttcttg catctcgaca aagtaaaggt tcggatcgtt tgccatcaat
     1501 cccttcctgt atttatccct aacttgccgg ggttcataag tcatgatccg actttccgcc
     1561 gtcatcatca ccttcaacga ggaaaagaac atcggcagat gcctggagtc gctcaagggc
     1621 gtggcggacg agatcgtcgt gctggactct ttctcgaccg accagaccga atcgatctgc
     1681 cgggagcacg gagtccgttt cattcgtcat ccttttggcg gttacattga acagaagaac
     1741 gaagtactgc gcctggcgca ctacgatcat gtgctctccc tggatgctga cgaagcacta
     1801 tcccctcagt taagagcttc catcctggac ctgaaggcga actggcagtt ggatgcttac
     1861 tcgttcaacc gcctgacctg gtattgccga cgtttcatgc gacatggtgg ctggtatccc
     1921 gatacgaaag tgcgcctctt cgtccggggc aagggcgcct ggggtgggga gaacccacac
//
LOCUS       DAOTON010000565         1974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_44765_length_1974_cov_2.669620, whole genome shotgun sequence.
ACCESSION   DAOTON010000565 DAOTON010000000
VERSION     DAOTON010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44765_length_1974_cov_2.669620"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="P5292_10450"
     CDS             complement(<1..463)
                     /locus_tag="P5292_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HRS39589.1"
                     /translation="MYDILELNDKLVNELKEIARLMNIPNYDELRKQELIYKILDQQA
                     LNPTASNSIRESLVGKFSNAKAESVKVAAPAAAPAAKESDSAGSRPKRRRVPPGAAAA
                     DAPAPVALEPSVKVAPARNPNPTPPPPPPTPVPAEAAPTPQPSQPRHPNQDQ"
     gene            784..1230
                     /locus_tag="P5292_10455"
     CDS             784..1230
                     /locus_tag="P5292_10455"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HRS39590.1"
                     /translation="MIQRIQTLFFLGVVLISVLLIFVPVYELPDIQVPPNPDGTPAIQ
                     AISIANNALLMLLNGAVGVFALVAIFLYRNRNLQARIGNLMLLLLCIEVGLLFFVADT
                     MATNLDQRLHYRYGSYLPLVSLLFTFLAVRFVKRDEALIRSADRLR"
     gene            complement(1235..1744)
                     /locus_tag="P5292_10460"
     CDS             complement(1235..1744)
                     /locus_tag="P5292_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008865728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRS39591.1"
                     /translation="MRRVLLLSDTHGTIDDRIMRRVELADEIWHAGDIGSMEVADQLE
                     HQRPVRAVYGNIDDHRMRLRFPEHAIFQCEELKVLITHIAGTPGHYPASVRTLLEKHR
                     PDLFVCGHSHILKVQRDEKFGHLHLNPGAAGKHGFHKVRTLLEFVVDGKSIRELQAVE
                     IGLRGSLEP"
     gene            complement(1749..>1974)
                     /locus_tag="P5292_10465"
     CDS             complement(1749..>1974)
                     /locus_tag="P5292_10465"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRS39592.1"
                     /translation="AGRESNRQVSRHDEAIILSPSVYVKSSPDEKGNDLFILHEGTKV
                     SLQDQVGNWQEIRLANGTVGWVTLNVLEAI"
BASE COUNT          404 a          520 c          548 g          502 t
ORIGIN      
        1 gctgatcctg gttcggatga cgcggttgcg acggttgtgg tgtcggtgca gcttcggctg
       61 ggacaggagt cgggggcggt ggtggcggag tggggttcgg atttcgagcc ggtgcgactt
      121 taacggatgg ttccagcgct accggcgctg gagcgtctgc agcggcggca ccgggaggca
      181 cgcgacgccg cttgggccgg gaaccggcac tgtcgctttc cttggcggcc ggggcagcag
      241 ccggagctgc aactttgacg gattccgctt tggcgttgct gaacttaccc accagggatt
      301 cccggatgga gttgctcgca gtcggattga gcgcctgttg gtcgaggatc ttgtagatca
      361 actcctgctt ccgaagttca tcgtagttcg gaatgttcat caaccgggca atctctttca
      421 gctcgttaac gagcttgtca ttcaattcaa ggatatcgta catgaaaata ctattggtga
      481 aatacgaagc gcagattcgc gtatacgttc aggatataat cgggggagac cggaaaaccg
      541 ggtggaattt tctccgatag gagagaagta ctcagaaata acggggatga atcagctgaa
      601 ctataggaaa gggaagagga tgtaatcgaa atcgcttccc gggcgctaaa ttcatcacaa
      661 gtataggtgc ttttttcaat tggtgcaaac aatttatgaa caaattttgc agattttgag
      721 gctgaggtaa ccgatttgcc cttttaaatg ggtttatcat ctttgtcccc gatccatccg
      781 tctatgatac aacgcatcca aaccctgttc ttccttggcg tcgtcctgat atccgtctta
      841 ctgatctttg tcccggtata cgaattaccg gatattcagg taccgccgaa tccggatggc
      901 actccggcaa tacaggcgat ttccattgcc aacaacgcgc tactgatgtt gctcaacggc
      961 gcggtcgggg tcttcgcact cgtggctatt tttctttacc gcaacaggaa cctccaggcg
     1021 cgcatcggta atctcatgct gctcttgctt tgtatcgaag ttggcttgct gtttttcgtg
     1081 gccgatacga tggcaaccaa cctggatcaa cggctccatt accgttatgg tagttatctc
     1141 ccgctggtgt cgctgctctt tacctttctg gcggttcgct tcgtcaagcg cgatgaggcc
     1201 ctgatacgct ctgctgaccg gttgcggtga acggttaggg ctccagcgac ccgcgtaaac
     1261 cgatttcgac agcctggagt tcccggatcg attttccatc gacaacgaac tccagcaggg
     1321 tgcggacttt gtgaaagccg tgcttgccgg ctgctcccgg attcaaatgc aaatgaccga
     1381 atttttcatc ccgttgtact ttcaggatgt gggaatggcc gcagacgaac aggtcggggc
     1441 gatgcttttc caataatgtt cggacgctgg ccgggtaatg accgggtgtc ccggcaatgt
     1501 gcgtgatcag caccttcagc tcttcgcatt gaaagatcgc atgttccggg aatcgtagcc
     1561 gcatccggtg atcatcaatg ttaccgtaca ccgcccggac cgggcgttga tgttcgagtt
     1621 gatcggccac ctccatggat ccgatatcac cggcatgcca gatctcatca gcgagttcga
     1681 cccggcgcat gatccgatcg tcgatcgtac cgtgcgtatc actcaggagc aagaccctgc
     1741 gcatccgttc agatcgcttc caggacgttc aaggtcaccc aaccgacggt tccattcgcc
     1801 aggcgaatct cctgccagtt gccgacctga tcctgcaggg aaacctttgt tccttcgtgc
     1861 aggatgaaca ggtcgttgcc tttttcatcg ggagagcttt taacatatac gctgggagac
     1921 aggatgatcg cctcatcgtg tcggctcacc tgccgattac tttcgcgacc cgcc
//
LOCUS       DAOTON010000566         1970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_44898_length_1970_cov_2.515927, whole genome shotgun sequence.
ACCESSION   DAOTON010000566 DAOTON010000000
VERSION     DAOTON010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44898_length_1970_cov_2.515927"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..437
                     /gene="rbfA"
                     /locus_tag="P5292_10470"
     CDS             42..437
                     /gene="rbfA"
                     /locus_tag="P5292_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRS39593.1"
                     /translation="MDSTRQLKYARLIQKEIGDIFQREGPDFYGKAFVTVMGVRVSPD
                     LGIARVYLSVFGAKDPQGTIDQIESHNKEIRKRLGNRIRHQARIIPELKFFLDDSLDY
                     AEKIDKLLRDSKGKDDERSVDPGENSSEQ"
     gene            551..1372
                     /locus_tag="P5292_10475"
     CDS             551..1372
                     /locus_tag="P5292_10475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS39594.1"
                     /translation="MQYDPIKRSLGNVFNKTPFLRKIFYRMLDLLLLRAWHVHKELRS
                     LTPELLRRERDGGVQVLDAGAGFGQYSYWINRKHPAWNILALDVKDEQVADCNRFFSA
                     IGAKNVRFEVGDLTTYVKAGTYDLIVCVDVMEHIEPDVQVFRNYHASMKNGGVLLIST
                     PSDQGGSDVHGEGEASFIEEHVRDGYNIGEIAEKLRSAGFSRIEARYQYGSPGKISWR
                     LSMKYPIQLLGVSKLFFIIIPFYYLIAYPIAYVLNWADVSGNHKTGTGLIVKAWK"
     gene            1406..>1970
                     /locus_tag="P5292_10480"
     CDS             1406..>1970
                     /locus_tag="P5292_10480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS39595.1"
                     /translation="MRLPLFIARRYFFSRRSQRAVNIISMISVTGISIGTAALIVVLS
                     VFNGFEDLILRLYNSFDPDIKIEAASGKRIDPLVLPAERLLQVPGVQTVCPVVEENCL
                     VRYRDRQYIATMKGVGDDYARLTGIDTLLLEGNFVLDSAGSEYAVVGGAIAYTLQLHT
                     GDLLNQLEVYAPKREAGSLLVPEEAFNR"
BASE COUNT          451 a          572 c          532 g          415 t
ORIGIN      
        1 cggcctgccc tgtcccggga aatccctacc ttcgcaccgc gatggacagt acccgacagc
       61 ttaagtatgc ccgcctgatt cagaaggaga taggcgatat ttttcagcgg gaaggcccgg
      121 atttttacgg gaaagccttt gtgacggtga tgggcgttcg ggtgagtccc gacctgggca
      181 tcgcacgggt gtatctgagc gtattcggcg cgaaggatcc gcaagggacc atcgaccaga
      241 tcgaaagtca caacaaagag atccgcaagc gcctcggcaa ccggatccgc caccaggcgc
      301 gcatcatacc ggagctcaaa ttcttcctcg acgattccct cgactatgcg gagaagattg
      361 acaaattgct cagagattcg aaagggaagg atgacgagcg ttcggttgat ccgggtgaaa
      421 acagcagtga acagtgaata gcgaatagcg aatagaagat acggagatcc agaataaact
      481 cgaaacccga aacgggaaac ccacaacccc cttcctcgtc cctcggtctc gcccctcgcc
      541 cctttatcaa atgcagtacg atcccatcaa acgctctctc ggcaatgtct ttaacaagac
      601 gccgttcctg cggaagatct tttaccgaat gcttgaccta ctgctgttgc gggcctggca
      661 tgtccacaag gaacttcgtt cgctgacgcc tgaattgctc cgccgggagc gggacggtgg
      721 tgtgcaggtg ctggatgccg gcgcggggtt cggacaatac agctactgga tcaaccgtaa
      781 gcatccggcc tggaacatcc tggcactgga cgtcaaagac gaacaggtag ccgattgcaa
      841 ccgcttcttt tccgccatcg gcgcgaaaaa tgtccgcttc gaagtgggcg acctcacgac
      901 ctacgtgaaa gccggcacgt acgacctgat cgtctgcgtc gacgtgatgg aacacatcga
      961 accggatgtg caggtatttc gcaactacca tgcttccatg aaaaacggcg gcgtgttgtt
     1021 gatctcgacg ccttcggacc agggtggcag cgacgtacat ggagaaggcg aagcctcgtt
     1081 catcgaagaa cacgtacgcg acggatacaa catcggcgag atcgccgaaa aactgcgcag
     1141 cgccggcttt tccaggatcg aagcacggta ccagtacggc agtccgggca agatctcctg
     1201 gcgactgtcg atgaagtacc cgatccagtt gctgggtgtg agcaaactct tcttcatcat
     1261 catcccgttc tattacctca tcgcctaccc catcgcttat gtgctgaact gggccgatgt
     1321 atccggtaat cacaaaaccg gcaccggcct gatcgtgaaa gcctggaagt aaggcggctg
     1381 ggattcgtat cttcaccggg tcagcatgcg cctgcccctt ttcatcgccc gacggtattt
     1441 cttttcccga cgctcccagc gcgcggtgaa catcatctcg atgatatcgg tcaccggtat
     1501 ctcgatcggg acagcggcat tgattgtggt gctttccgta ttcaacggct tcgaggatct
     1561 tattctccga ctctacaatt cctttgatcc cgatatcaag atcgaagccg catcgggtaa
     1621 acggatcgat cctctcgtgt tgcctgccga acgcctgctc caggtacccg gagtccagac
     1681 cgtctgcccg gtcgtggaag aaaactgcct cgtccgctac cgcgaccgac aatacatcgc
     1741 gaccatgaaa ggcgtcggcg acgattatgc acgactgacc ggtattgaca ccttgcttct
     1801 ggaaggaaat ttcgtgctcg actccgctgg ctcggagtat gcggtggtcg gaggcgcgat
     1861 tgcctatacc ctgcaattgc ataccggcga cctgctcaat caattggaag tctatgctcc
     1921 caaacgtgaa gccggctcgc tgctcgtccc ggaagaagcg ttcaaccgcc
//
LOCUS       DAOTON010000567         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_45115_length_1963_cov_3.253669, whole genome shotgun sequence.
ACCESSION   DAOTON010000567 DAOTON010000000
VERSION     DAOTON010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45115_length_1963_cov_3.253669"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="P5292_10485"
     CDS             <1..290
                     /locus_tag="P5292_10485"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRS39596.1"
                     /translation="NLIMNAPLVVGASGCVFGLLLAYGMLFPNNVIYIYFALPIKAKY
                     FVILYGAIELFSGIANVRGDNVAHFAHLGGLITGFLLIQFWRNQNRRKFRY"
     gene            300..1175
                     /locus_tag="P5292_10490"
     CDS             300..1175
                     /locus_tag="P5292_10490"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRS39597.1"
                     /translation="MSTGWKDMWQNQVRSADLLQRLLMVNIALFILLHLVHSLSALFL
                     HPMLDFATASRWLAVPAALPVLVVKPWSLLTYQFFHWDFFHLLFNMLWLYWMGKIFQE
                     YLGAPKLFGTYLLGGLSGAVFYIVFYNVFPLFADGVRDSFALGASASVLAVTIATATL
                     LPDYPIQLLLFGTVRLKWIALITILLDLINISGSNAGGHIAHLGGAFFGFIYIRQLQS
                     GRDLSAWLQRMFFRKGGTGRMKVKYRRSDDDFLSDKKSKQERLDEILDKINRSGYGSL
                     TKEEQEFLFHASKDS"
     gene            1246..>1963
                     /locus_tag="P5292_10495"
     CDS             1246..>1963
                     /locus_tag="P5292_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39598.1"
                     /translation="MKRLFWLLNLLAGIGLLLSFVAAWVSPASVWWLALFGLGFGTLY
                     LVNFLFFLGWKLAHSRRYWLSLALLLLGAGKLFGMYQFPFGGKEEPALAERNRSLKVM
                     SFNVRLFNLYNWFHNQETRQAIFRYLERSSPDIVCFQEYYQSDDPKENYHNTDSLYRI
                     LKTTFAHVEYTVTLRETHHWGIATFSKYPIVRQQAVHFKKKGGNIFLLTDIRVGDDTL
                     RVFNTHLESIRFRWEDYQFIR"
BASE COUNT          411 a          502 c          507 g          543 t
ORIGIN      
        1 agaacctgat catgaacgcc cccctggtgg ttggtgccag cggttgtgtc ttcggtttgt
       61 tgttggccta cgggatgttg tttccaaaca atgtcatata catctatttc gcgttaccca
      121 tcaaagccaa gtacttcgtg attctttacg gcgcaatcga attgttttcc ggcattgcca
      181 atgtcagggg cgacaacgtt gcacacttcg cgcatcttgg cggactgatc accggattcc
      241 tcctgattca attctggcgg aaccagaacc gtcggaaatt tcgttattga taccctgaga
      301 tgagtactgg ctggaaggat atgtggcaaa accaggttcg gtcggcggac ctcttgcagc
      361 ggctgttgat ggtcaatatc gcgctgttca tcctcctgca tttggtccat agcctctcag
      421 cattgttcct gcatccgatg ctggacttcg ccactgcatc acgctggctg gcggttccgg
      481 cagctttgcc ggtactggtg gtcaaaccct ggtccctgct gacctatcag ttctttcatt
      541 gggatttctt tcacctgctc ttcaacatgc tctggttgta ttggatggga aagatctttc
      601 aggagtacct gggcgctccg aaattgttcg gtacctattt gctcggcgga ttgagcggag
      661 ccgtctttta catcgtgttc tacaatgttt ttccgctctt tgctgatggt gtccgggatt
      721 cgtttgccct gggagcctcg gccagtgtac tggctgtcac catcgcgacc gctaccctcc
      781 tgcccgacta tccgatccaa ctgcttttgt tcggcacggt ccggctgaag tggatagccc
      841 tcatcacgat tctactggac ctcatcaaca tttccggttc caacgcaggc ggtcacatcg
      901 cccacctggg aggcgcgttc ttcggcttca tctatatccg gcaattgcag tcgggccgtg
      961 acttaagtgc ctggctccag cgaatgttct ttcgaaaggg aggcaccgga aggatgaagg
     1021 tgaagtatcg tcgatccgac gatgatttcc tttccgacaa gaaatcgaaa caggaacgac
     1081 tggacgagat cctcgacaag atcaaccggt ccggttatgg cagcctgacg aaggaggaac
     1141 aggaattcct attccatgca agtaaagaca gttgacttca ttgtcaggca aattttgttt
     1201 ctttaagtcg tgagtggcgc ctctgttccg cgtaccggcg gtattttgaa aagactcttc
     1261 tggttactca acctgctggc aggtatcggc ctgctgcttt ctttcgttgc ggcatgggtg
     1321 agcccggcct ccgtctggtg gcttgcattg ttcggactcg gtttcggaac gctctatctg
     1381 gtcaattttc tatttttcct tggttggaaa cttgcccata gtcgtcgata ctggctctcc
     1441 ctggcgttac tgttgctggg ggcagggaaa ctattcggca tgtatcagtt tccctttggt
     1501 ggaaaggaag agccagcttt agctgagcgt aatcgatcgc tcaaggtgat gtcgttcaat
     1561 gtccgtctgt tcaacttgta caactggttc cataaccagg aaacgcgaca ggccatattc
     1621 cggtatctgg aacgatcctc tccggacatt gtttgttttc aggagtacta tcagtctgac
     1681 gatccgaagg agaactacca caatacagat agtctataca gaatactgaa aacaaccttt
     1741 gcccatgtgg aatacaccgt cacgctaagg gaaacgcatc actggggtat cgcgacgttc
     1801 agcaagtacc cgattgtccg acagcaagcg gtgcatttca agaaaaaagg tggcaacatt
     1861 ttcctgctca cggatatccg ggtcggagat gatacgctgc gggtattcaa cacgcacctg
     1921 gaaagcatcc ggtttcggtg ggaggattac cagttcatcc gta
//
LOCUS       DAOTON010000568         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_45176_length_1961_cov_3.535152, whole genome shotgun sequence.
ACCESSION   DAOTON010000568 DAOTON010000000
VERSION     DAOTON010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45176_length_1961_cov_3.535152"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="P5292_10500"
     CDS             complement(<1..1703)
                     /locus_tag="P5292_10500"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRS39599.1"
                     /translation="MLETLARIILRYRVASLVILLGITVFMGYTATKVQLSYEGAKIL
                     PASDSTYAEYLKFKQKFGEDGTVMVIGFQDQKMWQAKTFGDWYDLTESVKKVEGIQEV
                     LSIARAFQVIRNDSSQKLDFKPVITARPRTQAEVDSLHELLGRLPFYEGLLFNPETNA
                     TLMAITFDQKQLNTKDRIGIVRQIKEMADKFAVDNGLTLHYSGLPYIRTAVTEKIAGE
                     MKIFVALALLITALILMLFFRSVQAVLFPMIVVIFGVIWSFGTLVLFGYKITILTSLL
                     APLIIVIGIPNCILLLNMYQQEFQKHGNKIKALVRTIHRIGASTFFANVTTSVGFLVF
                     AFTHSAVLIEFGIITAINVMLTYLISLFLIPIVFSFLPPPSAKQIKHLDRRAINRILH
                     RVDHWVHHYRWRVYATVLVVIGISLYGANKITTVGYVVDDLPKKDVIYTDLKFFESNF
                     RGVLPFEISVDTREPGGALKLKTLYKINRLQKLLAQYPQFSEPVSVAEGIKFSYQGLN
                     DGDPKYYIIPNVEELARLSSYAGTAKGNQRMFRSIIDSTQQVTRVSFQVADIGSIKMK
                     SL"
     gene            complement(1736..>1961)
                     /locus_tag="P5292_10505"
     CDS             complement(1736..>1961)
                     /locus_tag="P5292_10505"
                     /inference="COORDINATES: protein motif:HMM:NF021546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRS39600.1"
                     /translation="GVTIFKGKSGYGNRGDRNHEIDVIFTVVTRLEIHRLKGLVERLD
                     PKAFLVTHSINDSRGGMIRKRPQFLPQAHS"
BASE COUNT          439 a          496 c          571 g          455 t
ORIGIN      
        1 agggatttca ttttgataga accgatgtcg gccacttgaa aactcacccg ggtcacctgt
       61 tgggtactgt cgatgatgga ccggaacatg cgttgatttc ccttggcggt accggcatag
      121 ctggaaaggc gcgcgagttc ctcaacattc ggaatgatat agtacttcgg gtctccgtcg
      181 ttgagaccct ggtaggaaaa tttgatgccc tccgctaccg agaccggttc ggaaaattgc
      241 gggtattgcg cgagcagttt ctgaagccgg ttgatcttgt aaagcgtctt cagtttgagc
      301 gcgccacccg gctcccgtgt gtcgacgctg atctcgaacg gcagcacacc ccggaaattg
      361 ctctcgaaga atttcaagtc ggtgtagatg acgtctttct ttggaagatc gtcgaccaca
      421 taaccgacgg tggtgatctt gttggcgccg tagaggctga tcccgattac caccaatacc
      481 gtcgcgtaca cgcgccagcg ataatggtgt acccagtgat cgacacgatg caggatgcgg
      541 ttgatggcgc gtcggtcgag gtgcttgatc tgctttgccg aaggcggtgg caggaaactg
      601 aaaacgatcg ggatgaggaa gagcgagatc aggtacgtca gcatcacatt tatggcggtg
      661 atgatgccga attcgatcag gactgcgctg tgtgtgaagg cgaaaacgag aaaaccgaca
      721 gaggtggtga cattggcgaa aaaggtgctg gctccgatcc ggtggatcgt gcgcacgagg
      781 gctttgatct tgttgccgtg cttctggaat tcctgttggt acatgttgag caacaggatg
      841 caattcggaa tgccgatgac aatgatcagc ggagccagca ggctggtcag gatggtgatc
      901 ttgtacccga agaggacaag cgtaccgaac gaccagatga ccccgaatat caccacgatc
      961 atcgggaaga gcacggcctg cacgctgcgg aagaacagca tgaggatcaa agccgtgatc
     1021 agcaacgcga gcgcgacgaa gatcttcatc tctccggcga tcttctcggt caccgcggta
     1081 cggatatagg gcagcccgga ataatggagg gtcagaccgt tgtccactgc gaacttatcc
     1141 gccatttcct tgatctgccg gacgatcccg atgcgatctt tggtgttgag ttgcttctga
     1201 tcgaacgtga tcgccatcag ggtcgcgttc gtttccggat tgaagagcaa accttcgtaa
     1261 aatggcagtc gaccgagcag ttcatgcagg ctgtcgacct ccgcctgggt ccggggtctt
     1321 gcggtaatga ccggtttgaa gtccagcttc tgtgagctgt cgttccgaat aacctgaaaa
     1381 gcgcgggcga tcgagagcac ctcctgtatg ccctctacct ttttgaccga ttccgtcaga
     1441 tcgtaccaat caccgaaggt cttggcttgc cacatcttct gatcctggaa gccgatgacc
     1501 atgacggtcc cgtcttcgcc gaacttctgt ttgaacttca ggtattcggc gtaggtggag
     1561 tcgctggccg gcaggatctt cgctccttca tagctcagtt ggaccttggt ggccgtataa
     1621 cccatgaaca ccgtgatacc cagcaggatg accagtgaag ccacccggta gcgcagtatg
     1681 attcgggcaa gggtttcgag catagacggg taaatgtatg aaaactaccc taagctcagg
     1741 aatgggcctg cggcaggaac tgtggacgtt tgcggatcat accgccacgc gaatcgttga
     1801 tgctgtgtgt gaccaggaac gccttcggat cgagccgttc caccaggcct ttgagccggt
     1861 gaatttcaag tcgtgtaacg acggtgaaaa tgacatcgat ctcatggttc cggtcgccgc
     1921 ggtttccata tccgctttta cccttgaaga tggtaacgcc a
//
LOCUS       DAOTON010000569         1956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_45342_length_1956_cov_2.353498, whole genome shotgun sequence.
ACCESSION   DAOTON010000569 DAOTON010000000
VERSION     DAOTON010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45342_length_1956_cov_2.353498"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /gene="lnt"
                     /locus_tag="P5292_10510"
     CDS             <1..1251
                     /gene="lnt"
                     /locus_tag="P5292_10510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRS39601.1"
                     /translation="GFEYLHLNWDISWPWLTLGNVFASHPEWVQWYEYTGTLGGSLWV
                     LGVNLLIFQIIKNVFGRELLAKLRRINTLVLSANMVGLILLPLFFSVWRYQRVSDKGE
                     PVNVVVVQPNIDPYNEKFNGTGSAQVAKLLQLASTEADSTTDLIIGPETALPDGLWEE
                     QIGIDKDVRTIRTWLQDYPKASFLAGLSSYKQYQAGEPVSATARKFKDADAYYDAYNS
                     AMLLDRNGNLQLYHKSRLVPGVEKMPYPKLFGFLEDYAIELGGTSGSLGMQKNRTNFE
                     TASGLKIAPAICYESIYGDFLRAYFRGGAGFLAVITNDGWWGNTPGYRQHFEYARLRA
                     VEFRKCVARSANTGISCFINQRGDVVAQSGWWEDAVLKHTLLANDRVTFYARHGDYIG
                     FLCAFLAVSLLSFVLFRKLISRSR"
     gene            1338..>1956
                     /locus_tag="P5292_10515"
     CDS             1338..>1956
                     /locus_tag="P5292_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS39602.1"
                     /translation="MNSYDVIVIGAGPGGYVAAIRCAQLGMKTALIERYSTLGGTCLN
                     VGCIPSKALLDSSEHFHNAAHTFKTHGIELKDLQVNFSQMIARKAEVVKQNTEGIRFL
                     MKKNKIEVYHGHGSFLSSTRLSVKADDGKVEELETKNVIIATGSKPASLPGITIDKER
                     VITSTEALNLNEVPKHLIIIGGGVIGLELGSVYARLGAKVSVVEYL"
BASE COUNT          409 a          566 c          568 g          413 t
ORIGIN      
        1 ggattcgaat accttcacct taactgggac atctcctggc cctggctgac gctgggcaat
       61 gtctttgcct cgcatccgga atgggtgcag tggtacgagt acaccggtac gctgggcggt
      121 tcgctctggg tgttgggggt gaacctgctg atctttcaga tcatcaagaa cgtcttcgga
      181 cgcgaactgc tggccaagct gcgccggatc aacaccctgg tgttgtcggc caacatggtg
      241 ggcctgatcc tgctgccctt gttcttctcg gtctggcgtt atcagcgtgt aagcgacaag
      301 ggcgagccgg tgaacgtggt cgtggtgcag ccgaacatcg atccgtacaa cgaaaaattc
      361 aacggcaccg gctcggcgca ggtggccaag ctgctgcagc tcgcttcgac cgaagccgat
      421 tcgaccaccg acctgatcat cggaccggag acggccttgc cggacggttt gtgggaggaa
      481 cagatcggga tcgacaaaga cgtgcgcacg atccggacct ggctacagga ttatccgaag
      541 gcctcgttcc tcgccggttt gtcatcctac aaacaatacc aggccggtga accggtgagc
      601 gccaccgcgc gtaagttcaa agacgcggac gcttactacg acgcctacaa cagcgcgatg
      661 ctgctcgacc ggaacggcaa tcttcaattg taccataagt cgcggctggt ccccggcgtg
      721 gagaagatgc cttatccgaa gctcttcggc tttttggaag actacgcgat tgagcttggg
      781 ggcacttccg gcagcctggg catgcaaaag aaccgcacga acttcgagac tgcatccggc
      841 ttgaagatcg cgccggcgat ctgctatgaa tccatttacg gcgacttcct gcgcgcctac
      901 ttcaggggcg gcgcgggctt tttggcggtg atcaccaacg acggctggtg gggcaacacc
      961 ccgggttatc gtcagcactt cgaatatgcg cgcctgcgcg cggttgaatt ccggaagtgt
     1021 gtcgcacgat cggccaacac gggcatttcc tgtttcatca accaacgcgg ggatgtggtc
     1081 gcgcagtcag gctggtggga ggatgcggtg ctgaagcaca ccctgctcgc caacgaccgc
     1141 gtgacctttt acgcccggca tggcgattac atcggcttcc tttgcgcgtt tttagctgtc
     1201 agcctcctgt cgtttgtcct cttccggaag ctgatctccc ggagccgttg aatcggcggg
     1261 aatcccctac ctttgcagcg aaaatcggcc cggaaacgaa ctttttcggg tttttcgggt
     1321 tttcctgact acctaccatg aactcctacg acgtcatcgt catcggcgcc ggtccgggtg
     1381 gctatgtagc cgccatccgc tgcgcgcaac tgggcatgaa gacggccctg atcgaacgct
     1441 attccaccct cggtggcacc tgcctgaatg tcggttgcat tccctcgaaa gccctgctcg
     1501 attcctcgga gcactttcac aacgcggcgc atacgttcaa gacgcacggc attgagttga
     1561 aagacttaca ggtcaacttt tcgcagatga tcgcgcgtaa agccgaggtc gtgaaacaga
     1621 acaccgaagg catccggttc ctgatgaaga agaacaagat cgaggtgtat cacggtcatg
     1681 gctccttcct ttcatcgacc aggctgagcg tgaaagccga cgacgggaaa gtcgaggaac
     1741 tggaaacgaa gaacgtgatc atcgccaccg gatcgaagcc ggccagtctg cccggcatta
     1801 ccatcgataa agaacgcgtg atcacctcca ccgaagccct taacctgaac gaagtaccca
     1861 aacacctgat catcatcggc ggtggagtga tcggactcga actgggttcc gtgtatgcac
     1921 gactgggcgc taaagtgagc gtggtcgagt acctgg
//
LOCUS       DAOTON010000570         1954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_45405_length_1954_cov_3.360716, whole genome shotgun sequence.
ACCESSION   DAOTON010000570 DAOTON010000000
VERSION     DAOTON010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45405_length_1954_cov_3.360716"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /locus_tag="P5292_10520"
     CDS             <1..1625
                     /locus_tag="P5292_10520"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRS39603.1"
                     /translation="NVLMGPSQSSNIQMINGSFSQTLSFTYVLQAVKEGTFKIGSAEI
                     VLNNQKVLSNPVTLTVTKGTSRSSGGSSGGGEPMTRNVFIRASVDKSNVFRGEGLVVT
                     YRLYTRVTLVNYTISKLPAPNGFWSQEIQLPQQLQFHNENVDGVNYQVADLKKMVLFP
                     QRSGNLELDPMEGEVIARVQVKRQRSNDPFDQFFGNDPFGSFFGGGLQDVKVPLKSQP
                     VRVNVRELPDGAPAGFTGAVGKFTFETSIDKDKTKTNEAVTLRIKVTGKGNIKLVDAP
                     KVNFPPDFELYDPKESVNITAGGSGASGSKTFEYLIIPRNAGNFKITVDPFSYFDLDK
                     KSYLELSGTTLDLAVEKGNETVTTTVSGVNKSDVQLLGKDIRFLKTADPQLALNPRPL
                     RGSWLFYVLLFMPFALLLIALQWRKKLAERMTNVALIRSQRASKVALRRLARSKELLG
                     KQDHAAFLEELSRALWGYISDKLQLPVSELNRDNAKTHLQQRGTTEAMINELLSVLDA
                     CEFARFAGGTASLPDDLYRRGIASITQMEEQA"
     gene            1622..>1954
                     /locus_tag="P5292_10525"
     CDS             1622..>1954
                     /locus_tag="P5292_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS39604.1"
                     /translation="MNRITRPSSFLIQLFCLLGLLAIDGTVLASGTDERYSTAGKAYE
                     KKEYATAIATYEELLREGYRAPDLYYNLGNAHFKAGHIAAAILNYERALKLAPDDEDT
                     RYNLRIANL"
BASE COUNT          492 a          530 c          508 g          424 t
ORIGIN      
        1 tcaacgtgct gatggggccc agccagtcga gcaatatcca aatgatcaac ggcagctttt
       61 cgcaaaccct cagttttact tatgtattac aggcggtgaa agaaggcacc ttcaagattg
      121 gttctgccga gatcgtattg aataaccaaa aagtactgag caatcccgtc acgcttaccg
      181 tgaccaaagg aacgtcgcgt tcttccggcg gcagttcggg cggcggcgag cccatgacca
      241 ggaatgtctt catccgcgcc tcggtggata agagcaatgt gtttcgcgga gaaggtcttg
      301 tggtgacgta ccgtctttac acccgcgtaa ccctcgttaa ctacaccatc agcaagttgc
      361 ctgctccgaa cggtttctgg agccaggaaa tccagttgcc ccaacaattg caattccata
      421 acgagaatgt ggacggggtc aactaccagg tggccgatct gaaaaaaatg gtcttgtttc
      481 cacaacgaag cggcaacctg gaactggatc cgatggaagg cgaagtcatt gcccgtgtac
      541 aggtgaaacg ccagcgcagc aacgacccgt tcgatcagtt tttcgggaac gatccgttcg
      601 gctcattctt cggcggagga ctgcaggacg tcaaagtgcc gctcaagagc caacccgtcc
      661 gggtgaacgt acgagaatta ccggacggcg cgcctgccgg ctttaccggg gccgtgggta
      721 agttcacctt tgaaacttct attgataagg acaagaccaa aacgaatgaa gccgtaacgc
      781 ttcggataaa agtcaccggc aaagggaaca tcaaactggt ggatgcaccg aaggtcaact
      841 tcccgcctga ttttgaatta tatgatccga aagaatcggt gaatatcacc gccggtggca
      901 gtggcgcaag cggtagtaag accttcgagt acctgatcat cccgcgcaat gccggtaatt
      961 ttaagatcac cgtagatccg ttcagctact ttgacctgga taagaagtcc tacctcgaac
     1021 tatccggtac gaccctggac ctcgcggtgg aaaaaggcaa cgaaacagtc actactaccg
     1081 ttagcggcgt gaacaaatcg gatgtacaat tactcgggaa ggacattcgt tttctcaaga
     1141 ctgccgaccc gcaactggcc ctgaacccac ggccgttgcg cggctcatgg ctgttctatg
     1201 tactgttgtt catgcccttc gcgctattac tcatcgcctt gcaatggcgg aaaaaactcg
     1261 ctgaacgaat gaccaatgtg gccttgatcc gcagtcaacg tgccagcaag gtcgctctcc
     1321 gtcgtctcgc cagatcgaag gagttgttgg gcaaacagga tcatgcggcc tttctcgaag
     1381 agctttcccg cgccttgtgg ggttatattt ccgacaagct gcaactgccg gtgtcggaac
     1441 tcaaccgtga taatgcgaaa acgcatctgc aacaacgcgg aacaacggaa gccatgatca
     1501 acgaattgct ttccgttctg gatgcctgtg aattcgccag gtttgcaggc ggaacggctt
     1561 ccctacccga cgacttatac cggaggggaa ttgcaagtat cacccagatg gaggaacaag
     1621 catgaaccgg atcacacgtc cttcttcctt tctgatccaa cttttctgcc tgctggggct
     1681 cctggccatt gacggtacgg tcctggcatc cggcacggat gaacggtatt ctacagccgg
     1741 gaaagcttat gaaaaaaagg agtacgctac ggccattgcc acctacgaag aattattgcg
     1801 cgaaggttat cgcgcgcccg acctctacta taatctcggt aacgcccatt tcaaagcggg
     1861 gcacatcgca gcggcgatcc tcaactacga gcgggcgctg aaacttgcgc cggatgatga
     1921 ggatacacgg tacaacctgc gcattgccaa cctc
//
LOCUS       DAOTON010000571         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_45476_length_1952_cov_2.748023, whole genome shotgun sequence.
ACCESSION   DAOTON010000571 DAOTON010000000
VERSION     DAOTON010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45476_length_1952_cov_2.748023"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="P5292_10530"
     CDS             <1..281
                     /locus_tag="P5292_10530"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HRS39605.1"
                     /translation="SVAAYFGPTLDLTILEQEEVYSKAQAEQVLRDFFQKDPPKSFQV
                     VHKGASREGLQYAIGTLQTASGKSYRTSFYIKSTNGKNFIQELRFETE"
     gene            363..1241
                     /gene="nadC"
                     /locus_tag="P5292_10535"
     CDS             363..1241
                     /gene="nadC"
                     /locus_tag="P5292_10535"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRS39606.1"
                     /translation="MTPTTPFPDTSPSGLSIPDFIRQALAEDIGDGDHTSLSTIPADK
                     QGKALVRVKQDGIIAGLVLADQILNTVDPTVIVKVLSNEGQSVSNGTVVMEVEGAVRS
                     LLMAERLLLNCMQRMSGIATMTRQFVEAVAGTGAIIVDTRKTTPNFRLFEKWAVLLGG
                     GQNHRFGLFDMILIKDNHVDAAGGIRPAIRRANDYLRSTGRQLPIEIETRNAAEVEEV
                     LAEGNVQRIMLDNFTTTDLQAAVARIAGRFETEASGGINLQTVRGFAETGVQFISVGA
                     LTHSYRSLDISMKILR"
     gene            1248..>1952
                     /locus_tag="P5292_10540"
     CDS             1248..>1952
                     /locus_tag="P5292_10540"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRS39607.1"
                     /translation="MTLRKLSRKILRKILTSGPVKSGVAYSKRVALPGFDNMPLYDVA
                     DFFFTGIQKGSIVTRAQSLAFSFFLATFPAIIFSFSLIAYIPIKGFQESLIRLLLETL
                     PQDAKDWTRETIVDIVRIQRGGLLSLGFLLALYFTTNGFMTMMRGFNSSYHILETRPP
                     WKQRLVALVLTLLLSTLVIIATTLIIFSEVATKFLVAKHILKSNTQVVLLLLGKWTVV
                     LALFLIAISFLYYYAPA"
BASE COUNT          422 a          578 c          504 g          448 t
ORIGIN      
        1 ggtcggtggc cgcatacttc ggccctaccc tggatctcac gattctcgaa caggaggagg
       61 tctattccaa agcacaggcc gaacaggttt tgcgggattt tttccagaaa gacccgccaa
      121 agtcgttcca ggtcgttcat aaaggggctt cccgggaagg tttgcaatac gccatcggca
      181 ccttgcagac ggccagcggt aagtcttacc gaacttcgtt ctatatcaaa tcaaccaacg
      241 ggaagaattt catccaggaa ttacgatttg aaacagaata ggtccaacga ttccgtattt
      301 acttccggcc gcttcacagc ggccggaact gtttatggtc cggaacctta tttttgcacc
      361 cgatgactcc gactaccccc ttcccggata cttctccttc aggtctgtcc attcccgatt
      421 tcatccgaca ggcactggcg gaagatatcg gtgacggcga ccatacttca ctatccacta
      481 ttcccgccga taaacagggt aaagccctgg ttcgggtgaa acaggacggg atcattgccg
      541 gacttgtact cgccgaccag atcctgaata ccgtcgaccc taccgtcatc gtgaaggtac
      601 tatcgaatga aggacagtcc gtttcgaacg ggaccgtggt catggaggtg gaaggcgctg
      661 tgcgatcgct tctcatggcc gagcgccttt tgttgaattg catgcaacgc atgagcggga
      721 tcgcaaccat gacccggcag ttcgtcgagg ccgtggccgg taccggcgcc atcatcgtgg
      781 atacacgcaa gaccactccg aatttccgcc tgtttgagaa atgggctgtc ttgctgggcg
      841 gcggacaaaa ccaccgcttc ggcttgttcg acatgatcct gatcaaggac aatcatgtcg
      901 acgcggcggg aggcattcgt cccgcgatcc gtcgcgcgaa cgactacctc cgttccaccg
      961 gcagacagtt gccgatcgaa atcgaaacgc gcaatgcagc ggaagtggaa gaagtgctgg
     1021 cagaaggtaa tgtgcaacgg atcatgttgg acaacttcac aacgaccgac cttcaggccg
     1081 ctgtagcacg catcgcgggc cgattcgaga ccgaggcttc cggtggtatc aacttgcaaa
     1141 ccgtcagagg attcgccgag accggtgtgc agttcatttc cgtaggcgcc ctgacgcata
     1201 gctaccgcag tctcgatatc agcatgaaaa tccttcgttg accgctcatg acccttcgga
     1261 aattatccag gaaaatcctc cgaaagatcc tgacctcggg tccggtcaaa tcgggtgtcg
     1321 cgtattccaa acgcgtggcg ctacccggct ttgacaacat gcccctgtac gatgtcgccg
     1381 atttcttctt caccggcatt caaaaaggtt ccatcgtgac ccgggcgcaa tcactggcct
     1441 tcagtttctt tctggctacg ttcccggcta tcatcttttc cttctccctg atcgcttaca
     1501 tcccgatcaa aggctttcag gagtcgctga tccggctgct gttggaaacc cttcctcagg
     1561 acgcgaaaga ctggacgcgg gagacgatcg tcgacatcgt acggatccag cgcggcgggt
     1621 tgctctccct cggtttcctc ctggccctgt acttcacaac gaacggcttc atgaccatga
     1681 tgcggggatt caactcctcc tatcacatcc tggaaacgcg accgccctgg aagcaacgcc
     1741 tggtcgcgct ggtgctcacg ctcctgttat cgaccctggt gatcatcgcc acgaccctga
     1801 tcatcttcag cgaggtggct acgaagtttc tggtagccaa gcacattctg aaatccaaca
     1861 cccaggtggt cttgctcctg ctgggaaaat ggacggttgt gctcgcgctc ttcctgatcg
     1921 cgatctcgtt cctgtactac tacgcgccgg ct
//
LOCUS       DAOTON010000572         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_45575_length_1949_cov_3.006336, whole genome shotgun sequence.
ACCESSION   DAOTON010000572 DAOTON010000000
VERSION     DAOTON010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45575_length_1949_cov_3.006336"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            396..1352
                     /locus_tag="P5292_10545"
     CDS             396..1352
                     /locus_tag="P5292_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRS39608.1"
                     /translation="MQLRKVLSFFFVLISGAAMAQDPQFTQFYANPLYLNPAFAGAQR
                     CPRVCLNYRNQWPALTGTFVTYTASYDQHVDPIGGLGLLVLQDKAGESTLTTTNVSGI
                     YSYQLNVTREFSIRLGLQGTYAQKRVDWNKLTFGDMIDPRYGFIYETQEQRPNETRNF
                     WDFSSGIVAYSNRYYGGVAVHHMTEPQEFFITEAPGSKLPMKITAHAGAIIPIAGNRD
                     GTTYISPNFLYQKQRDFQQYNVGFYIAKAPLVGGLWYRGSDSFIALVGLQQGIFKFGY
                     SYDVTTSKLYNASAGSHEISLGLQFGCHPKKKRFRTIKCPAF"
     gene            1424..>1949
                     /locus_tag="P5292_10550"
     CDS             1424..>1949
                     /locus_tag="P5292_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRS39609.1"
                     /translation="MKNLIRSSIGLVAGALLLTSCGKEKSSVTGWNYNDPKNGGFEVV
                     PYDEQETGPGLVLVEGGTFTMGRTEQDVTIDWNNIPRRVTVSSFYMDETEVANIHYLE
                     YLYWTSRVFSADYPEVYKKALPDTLVWRDKVGYNEPMIELYLRHPAYQQYPVVGVNWL
                     QANDFCNWRTDRVNE"
BASE COUNT          482 a          498 c          497 g          472 t
ORIGIN      
        1 gaagtgaacg ggtcgagctg ctctttttat cctgctgtgc ggaggcggga gcaagccggc
       61 atgccagtaa aaccaggagc aggcaagtac gggagaagaa agtgggcata ggaaggatgt
      121 gtcgaccgga tcgtctcaaa acgtggtccg gcttacaata ttgtaatcaa caaatttatc
      181 ccaatttggt tgccggagaa agcatcagat cagcaggaat gaggatgtcg gtaattggtt
      241 gaattattcc gtcagattcg tagttgatcc gtttaaaatt attaaaattg tacaaaatct
      301 tacggtacgt attagtgcgc acggttgaac gatctggccg cttgaaacca tgtatctttc
      361 aggccgtatg atgcgctaat acactctgac caactatgca gctccgcaag gtcctgtctt
      421 tctttttcgt cctgatttca ggggcagcaa tggctcagga tccgcaattc acgcaattct
      481 atgccaatcc gctgtacctg aatccggcat ttgccggtgc tcagcgctgt ccgcgggttt
      541 gcctcaatta ccgtaaccaa tggccggcct tgaccggtac gttcgttacc tacacggcgt
      601 cctacgacca gcatgttgat ccgatcggtg gattgggctt gttggtgttg caggacaaag
      661 ccggcgaatc aaccctgaca accacgaatg tttccggtat ctattcctac cagctcaatg
      721 tcacccgcga attctcgatt cgcctgggac tgcaaggcac atacgcgcag aagcgcgtgg
      781 attggaataa actgacgttc ggtgatatga tcgacccgcg ttatgggttc atttatgaga
      841 cgcaggaaca gcgcccgaac gaaacaagga atttctggga tttttccagt ggtatcgtag
      901 cctacagtaa tcggtattat gggggagtgg ccgttcacca catgacggaa ccccaggaat
      961 tcttcatcac cgaagctccc ggttcaaaat taccgatgaa gatcaccgcc catgccggtg
     1021 cgatcatccc gatcgccggt aaccgcgatg gcactacgta catatcgccg aatttcctgt
     1081 accagaaaca acgcgacttc cagcagtata atgtcggatt ctacatagcc aaagcaccgc
     1141 tggttggcgg actctggtat cgtggaagcg actccttcat tgccttggtg ggattgcagc
     1201 aggggatctt caaattcgga tacagttatg atgtgaccac ctcgaagctc tacaatgctt
     1261 cagccggttc tcacgaaatt tccctgggtt tgcagttcgg ctgtcacccc aaaaagaagc
     1321 ggttccggac catcaaatgt ccggccttct gattgcctta aatcgaagaa aataaatact
     1381 tttggcgctc gttagagcac catctattca aaccaaaaag cggatgaaaa acctcattcg
     1441 gagttcaatc ggcctcgtcg cgggagccct tctactgact tcctgcggaa aggagaaatc
     1501 ctccgttacc ggttggaatt acaacgaccc caaaaatggt ggtttcgaag tggttcccta
     1561 cgatgagcag gaaaccggac cgggactggt tctggtggag ggcggtacct tcaccatggg
     1621 ccgtacggaa caggatgtca cgatcgactg gaacaacatt cctcgtcgcg ttacggtcag
     1681 ttccttctac atggacgaaa cagaggtggc caacatccac tacctggagt acctgtattg
     1741 gacttcccgc gtattctctg ccgactatcc cgaagtttac aagaaggcgc ttcccgatac
     1801 gctggtttgg cgcgataaag tgggttacaa cgaacccatg atcgagctct acctgcgtca
     1861 cccggcctat cagcagtatc cggtggttgg tgtcaattgg ctgcaagcca atgacttctg
     1921 taactggcgt accgaccggg tcaacgaac
//
LOCUS       DAOTON010000573         1948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_45590_length_1948_cov_3.333862, whole genome shotgun sequence.
ACCESSION   DAOTON010000573 DAOTON010000000
VERSION     DAOTON010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45590_length_1948_cov_3.333862"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="P5292_10555"
     CDS             <1..203
                     /locus_tag="P5292_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004873.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRS39610.1"
                     /translation="YPGVEKTFAIQAGRELRVIVQSEKLSDKQSEVLSFDIANRIQNE
                     MTYPGQVKVTVIRETRSVNYAK"
     gene            282..1112
                     /locus_tag="P5292_10560"
     CDS             282..1112
                     /locus_tag="P5292_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015203741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS39611.1"
                     /translation="MNQEPFDLILEPRRSAGNYWRDLWQYRGLFYFLSWRDILVRYKQ
                     TVIGIAWSVIRPLLTMIVFTIVFGRLAQLPNEGVPYILLVCAAMLPWQFFANAFTESS
                     QSLIANAPMLTKVYFPRLIIPASAVVVALVDFFISLLILLGLMVWYRFVPGWQVVWLP
                     AFLLMAILVTMGAGFFIAALNVKYRDFRYIVPFIVQFGLYVSPVGFSSNVIPADWRWL
                     YALNPMVGVIDGFRWCLLGGSFRLEPTSFAISIALTILLFVFGLFYFRRMERSFADVV
                     "
     gene            1116..>1948
                     /locus_tag="P5292_10565"
     CDS             1116..>1948
                     /locus_tag="P5292_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006910319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS39612.1"
                     /translation="MSVAIRAEHLTKRYVLRHQVREPYTALRDVLARKARQAGKALLG
                     RNEQIITEDFHALNDVSFDILQGQRVGIIGRNGAGKSTLLKIISRITEPSEGRLFIKG
                     RVASLLEVGTGFHPELTGRENIFLNGSILGMTKAEISRKFDEIVAFSEVERFLDTPVK
                     RYSSGMYVRLAFAVAAHLEPEILLVDEVLAVGDAEFQKKCLGKMRDVAQSGRTILFVS
                     HNMAAIQNLCDTAIYLRNGQLVEMGEKDRVIATYLRSTADLHSIQLPIERTGPEAALS
                     DS"
BASE COUNT          401 a          515 c          540 g          492 t
ORIGIN      
        1 cgtaccccgg tgtagagaag accttcgcca tacaggccgg tcgcgaactc cgcgttatcg
       61 tccaaagcga gaagctcagc gataagcaat cggaggtttt gtcgttcgat atcgctaatc
      121 gcatccagaa cgaaatgacc tatcccggcc aggtcaaagt gaccgtcatc cgggagaccc
      181 gttcggttaa ttacgccaag taatacgata actttgactg gtaaccctcg acccttcggg
      241 cgcggaggtt accagtttca ttttacgccg ccactctccc catgaaccag gaaccattcg
      301 acctgatcct cgaaccgagg cgcagtgctg gaaactactg gcgcgacctg tggcagtatc
      361 ggggcctttt ctatttcctt tcctggcgtg atatcttggt ccgttacaag caaacagtca
      421 tcggtatcgc ctggagcgtg atccggccct tgttgaccat gatcgtcttc acgatcgtat
      481 tcggaaggct ggcgcaacta ccgaatgaag gcgttccgta catcttgttg gtgtgcgcgg
      541 ccatgttacc ctggcaattc ttcgccaacg cttttacgga atcgagtcag tcgttgattg
      601 ccaatgctcc catgttgacg aaagtctatt tcccccgcct gatcattccg gccagtgcag
      661 tggtggtggc gctcgtggac tttttcattt ccctgctgat cttgctggga ctcatggtgt
      721 ggtaccggtt tgtgccgggc tggcaggtcg tctggttgcc agcttttcta ctcatggcca
      781 tcctggttac catgggcgca ggatttttta tcgccgcctt gaatgtcaag taccgtgact
      841 tccgatacat cgtaccgttc attgttcagt tcggcttgta tgtgtcgccg gtcggtttta
      901 gcagcaacgt cattcccgcc gattggcgct ggctgtacgc cttgaatccg atggtgggag
      961 taatcgacgg atttcgctgg tgtctgctgg gagggagttt ccggctggaa cccaccagtt
     1021 ttgcaatctc catagccctt accatcctgc tcttcgtttt cggcttgttt tatttccgcc
     1081 ggatggagcg atccttcgcc gacgtagtct aaaccatgtc tgtcgccatt cgagctgagc
     1141 accttaccaa acggtatgtc ctgcgccacc aggttcggga accctatacc gcattgcggg
     1201 atgtattagc ccgtaaggcc cgacaggccg gtaaggcgct gctggggcgg aacgagcaaa
     1261 tcatcacgga agattttcat gcgctgaacg atgtgagttt cgatattctg caagggcaac
     1321 gggtcggcat catcggtcgc aacggagcag gaaagtcgac cttgttaaag atcatcagcc
     1381 gcatcaccga accatccgaa ggccgcttgt tcatcaaagg ccgtgtggcg agtttgctgg
     1441 aagtagggac cggctttcat cctgaactga caggccgtga gaacatcttc ctgaacggtt
     1501 ccatactggg catgacgaaa gcggagatct cccggaagtt cgacgagatc gtcgccttct
     1561 cggaagtgga acgctttctg gatacgcctg tgaaacgtta ttcttcgggt atgtatgtcc
     1621 ggctggcatt tgctgttgct gcgcatctcg agccggagat cctcctggtc gatgaagtgt
     1681 tagcggtagg agatgcggag tttcaaaaaa agtgtcttgg gaaaatgcgg gatgtagcgc
     1741 agtcggggag gaccatcctg tttgtgagcc acaacatggc cgcgatccag aacctctgtg
     1801 atactgcgat ctatttgcga aacggacaat tggtagagat gggagagaag gatcgggtga
     1861 tcgcgacata tctccgctcg accgctgatc tgcattccat ccaactgccg atcgaaagga
     1921 ccggaccgga agcggcgctg tcagattc
//
LOCUS       DAOTON010000574         1948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_45597_length_1948_cov_2.585843, whole genome shotgun sequence.
ACCESSION   DAOTON010000574 DAOTON010000000
VERSION     DAOTON010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45597_length_1948_cov_2.585843"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1058
                     /locus_tag="P5292_10570"
     CDS             219..1058
                     /locus_tag="P5292_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRS39613.1"
                     /translation="MSGTIPRHILKPPPGTIREIDLIDWAPRAKAFAPEKPHRHQFYE
                     LLIITGSGGEHDLNFSSLPMKKYEIHFVGPDDVHLVFREKASSGCTLLFLPGVIDRPL
                     LRSLPFGTERPVIQLPAAAFKRALIYLDLIRTETREQAAGNELVLRSLVQSLAIYLSR
                     FWERDRPGAALRVGNPLVDRFMLLVQRHYADHKRVEDYADLLHISPKHLIAVCKEHTG
                     NTPLNIIRVHLVAEIKKLLYHTDLSVKEIAYRLNFDEPGNFSKYFRSATGFTPLEYRA
                     GIR"
     gene            1145..>1948
                     /locus_tag="P5292_10575"
     CDS             1145..>1948
                     /locus_tag="P5292_10575"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRS39614.1"
                     /translation="MKSHLLACTCLLLSALDLSAQWVQKASGTSVALFDLYFPNRDTG
                     YVVGQDGVILRSFDVGESWSALSFPTSAGLNAVHFLNGHEGWVASDSGLYHTMDAGDS
                     WTLEPSLSRDGWYDIEFSDANTGFVSGEDGKIARTADRGQTWTILSTPLVRKLASLSF
                     PSAQVGYATGVGYNWQFLKTTDGGLNWVMDTIRPIGNICSLEAVFFTSETRGFIGGWY
                     IATFVGTDDGGVSWSDLATAGPVDLYSIHFPDSLHGFACASHSTFCRSQD"
BASE COUNT          403 a          553 c          517 g          475 t
ORIGIN      
        1 agatcacata cgggtcacgc gtttcccgcc agggcaattc ccgcgcgtgc tttttatacc
       61 aacgcaataa cgtaccgccg aatgccgcca taaatctcca cacaaggtac gacaagtgtg
      121 aattttgaat ccgtgatcat ccgcattatc cgtgtcatcc gtgtgcaaat aattcggagc
      181 ccaataggca tttccccgga atcagtattt tcgacaacat gtccggtacc atccctcggc
      241 atatcctgaa accgccgccc ggcacgatcc gggagatcga cctcatcgac tgggcgccgc
      301 gtgcaaaggc tttcgcgccg gaaaagccgc accggcatca gttctacgaa ctgctgatca
      361 tcaccggcag cggaggcgag cacgacctca atttttccag tctgccgatg aagaaatacg
      421 agatacactt cgtcggaccc gacgacgtgc acctcgtctt tcgtgaaaaa gcatcttcgg
      481 gttgcaccct gctgtttctt cccggcgtga tcgaccggcc cttgcttcga tccctacctt
      541 tcggtaccga gcgaccggtg attcagttgc cggctgccgc attcaaacgg gcgctcattt
      601 accttgacct gatccgtact gaaacgcggg aacaggctgc cgggaacgag ctggtgcttc
      661 gttcgctcgt gcaatcgctg gcaatctatt tgtcgcgctt ctgggaacgc gaccggccgg
      721 gagcggcttt gcgtgtggga aatccgttgg tcgaccgctt catgctcctc gtgcagcgac
      781 attacgcgga tcacaagcgg gtggaggatt atgcggactt gctgcacatc agcccgaagc
      841 acctcatcgc tgtttgtaag gaacataccg ggaatacgcc gctcaacatc attcgcgtcc
      901 atcttgtcgc ggagatcaaa aagctcctgt atcatactga cctttcggta aaggagatcg
      961 cttatcgcct gaacttcgac gagccgggta atttttccaa gtactttcgc tccgctaccg
     1021 gatttacgcc cctggagtac cgggccggta tccgttaggt taatttttta cccttgagtc
     1081 cgaggattca acctgtgggt cggaacaggc ctgatttatt tttgagtcac taaactccct
     1141 gatcatgaaa tcacatttgc ttgcctgtac atgcctgctc ctgtccgccc tcgacctttc
     1201 tgcccagtgg gttcagaaag catccggtac ttcggttgcg ttatttgatc tttattttcc
     1261 caatcgggat accggctatg tggtcggaca agacggtgtg atacttcgtt cgttcgatgt
     1321 aggagaaagt tggtcggcat tatcgttccc gacaagtgcc ggcttgaatg ccgttcactt
     1381 tcttaacgga cacgaaggtt gggtggccag tgacagcggc ctctatcata cgatggacgc
     1441 cggcgattcc tggaccctgg agccttcgct gtctcgcgac ggctggtacg atatcgaatt
     1501 ctccgatgcg aacaccggat ttgtctcggg agaggatggg aagatcgcac gtacggcgga
     1561 tcgcggacaa acctggacca tcctttcaac acccctggta cgtaagctgg catcgctatc
     1621 gtttcccagt gcacaggtgg gatacgcgac gggggtcgga tataactggc agttcctgaa
     1681 gaccaccgat ggcggactga actgggtgat ggataccatc cgcccgatcg gtaatatctg
     1741 ctcgctggaa gcggtctttt tcaccagtga gacccgcgga ttcatcggcg gttggtacat
     1801 cgccaccttc gtcggaacgg atgacggtgg tgtaagctgg agcgacctgg caaccgcagg
     1861 accggtcgat ctttactcca ttcatttccc ggatagtttg catggattcg cctgcgcctc
     1921 gcattcgact ttttgccgga gccaggac
//
LOCUS       DAOTON010000575         1945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_45681_length_1945_cov_3.340741, whole genome shotgun sequence.
ACCESSION   DAOTON010000575 DAOTON010000000
VERSION     DAOTON010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45681_length_1945_cov_3.340741"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="P5292_10580"
     CDS             complement(<1..1113)
                     /locus_tag="P5292_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRS39615.1"
                     /translation="MATRKEIKIKSNFSKIIISLASPEHILEQSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDWECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIT
                     LTVPVAHIWYFRSLPNKIGYLLGLPTKKLDMIIYYERYVVIQAGVKAQEGLNYLDFLT
                     EEEYLQHLETLPKENQHLDEKDPNKFIAKMGAEALYDLLSRLDLDGLSYSLRHQASTE
                     TSQQRKNEALKRLNVVEAFREANNHIENRPEWMIVKVVPVIPPELRPLVPLDGGRFAT
                     SDLNDLYRRVIIRNNRLKRLIEIKAPDVILRNEKRMLQEAVDSLFDNSRKVNAVKTES
                     NRALKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVV"
     gene            complement(1146..>1945)
                     /gene="rpoB"
                     /locus_tag="P5292_10585"
     CDS             complement(1146..>1945)
                     /gene="rpoB"
                     /locus_tag="P5292_10585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842951.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRS39616.1"
                     /translation="FNEVTGLYKRKKFALTVGDELPPGIVQLAKVYIAKKRKLKVGDK
                     MAGRHGNKGIVAKIVRDEDMPFLEDGTPVDIVLNPLGVPSRMNLGQIYETILGWAGEK
                     LGMKFGTPIFDGATIEQIDEYVSKAGLPKFGSTRLIDGLSGVQFDQPATVGVIYMMKL
                     GHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAFGAANILQEILTV
                     KSDDVIGRAKTYEAIVKGDNLPTPGIPESFNVLLHELRGLCLKVTLD"
BASE COUNT          400 a          536 c          540 g          469 t
ORIGIN      
        1 tacgacaata accgaacggg cggaataatc gacgcgttta ccgaggaggt tctgacggaa
       61 tcggccttgt ttacccttga gcgagtccga aagcgacttg agggcgcggt tcgattcggt
      121 cttgaccgca ttcaccttac gggaattgtc aaagagcgag tcaacggcct cctgcagcat
      181 gcgtttctcg ttacgcagga tcacgtccgg agctttgatc tcgatgagac gcttcagacg
      241 gttgttacgg atgatgacac gacgataaag gtcgttgagg tcagaggtgg cgaaacgacc
      301 accgtccagc ggaacgagtg ggcgcaattc cggaggaatc accggcacga ctttcacgat
      361 catccactcg ggacggttct cgatgtgatt gttcgcttcg cggaacgctt ccaccacatt
      421 caggcgcttg agagcttcgt tcttccgttg ctgggaagtt tcggtgctgg cctggtgacg
      481 gagcgaatac gaaaggccat ccagatcgag tcggctgagc aggtcgtaca aggcttcagc
      541 gcccatcttg gcgatgaatt tgttcggatc cttctcgtcg aggtgctgat tctctttcgg
      601 caaggtctcc aggtgctgga ggtattcttc ttccgtgagg aagtcgagat aattcaatcc
      661 ttcctgagcc ttgacaccgg cctggatcac cacgtaacgt tcgtagtaga tgatcatgtc
      721 gagcttcttg gtcggaagcc cgagcaggta accgatttta ttcggcaggg aacggaaata
      781 ccagatatgg gcaaccggca ccgtgagggt gatgtggccc atgcgctcgc ggcgcacttt
      841 cttttccgta acctcgacac cgcagcggtc gcaaacgatc cccttgtagc ggatacgctt
      901 gtacttgccg cagtgacatt cccagtcctt taccggaccg aagattcgct cacagaagag
      961 tccgtcacgc tccggcttgt aggtccggta gttgatggtc tccggcttga gcacctcacc
     1021 gctcgactgc tccaggatgt gttccggaga agcaaggctg atgatgatct tgctgaaatt
     1081 cgatttgatc tttatttctt ttctggtcgc catgttcgtt tcttgagagg ttaatgacgg
     1141 cctgatcagt caagtgtgac tttcaggcaa aggccgcgga gttcgtgcaa caatacgttg
     1201 aacgattccg gaatacccgg cgtcgggagg ttgtcgccct tcacgatggc ttcgtaggtc
     1261 ttcgcccggc cgattacatc gtcggacttg accgtcagaa tctcctgaag gatgttggcc
     1321 gcgccgaaag cttccagcgc ccaaacttcc atttccccga aacgctggcc accgaactgc
     1381 gccttaccac ccagcggctg ctgcgtgatg agcgagtacg gaccgatcga gcgggcgtgc
     1441 atcttgtcgt cgaccatgtg acccagcttc atcatgtaga tgacgccaac agtcgcaggc
     1501 tgatcgaact gtacgccgga gagaccatcg atcaggcggg tacttccgaa tttcggcagg
     1561 ccggctttcg aaacgtactc gtcgatctgt tcgatcgtag caccgtcgaa gatcggagta
     1621 ccgaacttca tgcccagctt ctcccctgcc catccgagaa tggtttcgta gatctgaccg
     1681 aggttcatac gcgacggtac gcccagcggg ttgagtacga tgtcgaccgg tgttccgtct
     1741 tccaggaacg gcatgtcttc gtcgcgaacg atcttggcaa cgataccctt gttaccgtga
     1801 cgtccggcca tcttatcacc gaccttcagc ttacgcttct tggcgatgta aactttggcc
     1861 aactgcacga ttccgggagg caattcgtca ccgaccgtga gcgcgaactt cttgcgctta
     1921 tacaggccgg tcacttcatt gaact
//
LOCUS       DAOTON010000576         1942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_45780_length_1942_cov_3.690514, whole genome shotgun sequence.
ACCESSION   DAOTON010000576 DAOTON010000000
VERSION     DAOTON010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45780_length_1942_cov_3.690514"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="P5292_10590"
     CDS             <1..1045
                     /locus_tag="P5292_10590"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRS39617.1"
                     /translation="GITALNIFLNIFFYLHLSQEKNIPFWKIFLLSTFFVFLSWEVTR
                     WVVLQVRKRFPGLQNMRTRIIRLTIYVTLATVALSIFKVWFQDAIEFYGEMNSGIFDY
                     FYGFGMNMFYALVITGIYESLYYFHQWQRLLAETELLKQANLQSQFDSLKNQVNPHFL
                     FNSLNSLSSLVEEDKEQAVSFIAELSSVYRYLLQSNERELTTLQSELDFIKAYFFLLR
                     TRFRQGVDLLIEVPDGMRERLLPPLTLQMLVENAVKHNVISVSRPLLVRITATSDGWL
                     HVVNNLQRKIQQVPGSGLGLVNITEKFRLLKQPQPQINETATEFRVSIPLMDSRMPYR
                     PFAGSAENVSLAS"
     gene            1063..1851
                     /locus_tag="P5292_10595"
     CDS             1063..1851
                     /locus_tag="P5292_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRS39618.1"
                     /translation="MKESLNLLIVEDEELAARKLNKMVLELEPNMCCLAITDSIAGTV
                     SWLKENGLPDLILMDIELADGQSFEIFSRVDVRCPVIFTTAYDEFALKAFKVNSVDYL
                     LKPVKPEELRTALEKFRSLRQSQISEGATQQNIGKLIETLVAQQQGTQYRERFLVRSG
                     QRLMPVSSDAIAYFFTEDKLVFLRTCDNRKFVLDYTLDELEHQLDPRRFFRANRQFIL
                     NSYCIEEVHTWFNGKLKVNVRPKTEEEVIVSREKAGDFKAWMGE"
BASE COUNT          457 a          538 c          499 g          448 t
ORIGIN      
        1 cggtattacc gcgctcaata tttttctgaa catcttcttc tatttgcacc tttcgcagga
       61 gaagaacatt ccattctgga agatatttct gcttagtacg tttttcgtct tcctgtcgtg
      121 ggaagtcacc cgctgggttg tcttgcaggt acggaaacgt tttcccggtc tgcagaacat
      181 gcgcacccgg atcatccgcc tgacgatcta cgtcaccctg gcaaccgtag cgttgtcgat
      241 tttcaaagtc tggttccagg acgcgatcga gttttacggc gaaatgaatt cgggcatctt
      301 cgactacttc tatggattcg ggatgaacat gttctacgcg ctggtgatca cgggcattta
      361 cgaatcgctg tactacttcc accaatggca acgactgctt gccgagacgg agttgctcaa
      421 acaggccaat ctccagagcc agttcgattc actgaagaac caggtcaatc cgcactttct
      481 tttcaatagc ctgaactcgt tgtcctcgct ggtggaggaa gacaaggaac aggccgtcag
      541 tttcatcgcg gaactgtcga gcgtctatcg ctatctcctg caaagcaacg agcgggagct
      601 caccaccctg caaagcgaac tggacttcat caaagcctat ttcttcctgc tccgcacgcg
      661 cttccgccag ggcgtcgacc tgttgatcga ggttcccgac gggatgcgag agcgtttgtt
      721 gccgccgctt accctgcaga tgctggtcga gaacgcggtc aagcacaatg tcatctccgt
      781 ttcacgtccc ttgcttgttc gcatcaccgc cacctccgac ggttggttgc acgtggtcaa
      841 taaccttcag cgtaagatcc aacaggttcc cggcagcggc ctcggtcttg tcaacatcac
      901 cgaaaagttc cgcttgctga aacaaccgca gccacagatc aacgagacgg caacagaatt
      961 ccgagtgagc attccgctca tggacagccg gatgccgtat cgtcccttcg ccggtagtgc
     1021 cgagaatgtc agccttgctt cctgaagtag ccttccctgc ccatgaaaga atcactgaat
     1081 ctcctgatcg tcgaagacga agaattggct gcccgaaagc tgaacaagat ggtcttggag
     1141 ctggagccga acatgtgctg cctggccatc acggacagta ttgccggtac ggtatcctgg
     1201 ctcaaggaaa acggattacc cgacctgatc ctgatggaca tcgaactggc cgatggacaa
     1261 agcttcgaga tcttttcccg ggtcgatgtc cgttgcccgg tcatcttcac aacagcatac
     1321 gacgaattcg cgctgaaggc gtttaaggtg aacagtgtcg attacctgtt gaaaccggtg
     1381 aagccggaag agttgcgaac ggccctggaa aaattccgct cgcttcgcca aagtcagatc
     1441 tcggaaggcg cgacccagca gaatatcggc aagctgatcg aaaccctggt cgcgcaacag
     1501 caaggaacgc aataccgcga acgctttctc gtacgatccg gtcagcgcct catgcccgtc
     1561 agttcagacg cgatcgccta cttctttaca gaggacaaac tggtctttct gcgcacctgc
     1621 gataacagaa agttcgtcct ggattatacc ctcgacgaac tcgaacacca gttagacccg
     1681 cgcaggtttt tccgggcaaa ccgacagttc attctgaaca gttactgtat cgaggaagtc
     1741 cacacctggt tcaatggaaa actgaaagtc aacgtgcgac ccaagaccga agaagaagtg
     1801 atcgtctccc gggaaaaggc aggggatttc aaggcgtgga tgggggagtg atggcggggt
     1861 gcgggttcct gccttcgcta aagcttcggc aggcaggcag gtttcgggtt cactgaaacg
     1921 ccgaaaatta cgtaattatc ct
//
LOCUS       DAOTON010000577         1941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_45816_length_1941_cov_3.402439, whole genome shotgun sequence.
ACCESSION   DAOTON010000577 DAOTON010000000
VERSION     DAOTON010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45816_length_1941_cov_3.402439"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..776
                     /locus_tag="P5292_10600"
     CDS             150..776
                     /locus_tag="P5292_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39619.1"
                     /translation="MPVRVGASLLAIVVFVLSCFLTQHRAMAQRLSPMAPLQNHPLER
                     KAFQSEVSVYDCIDIHETVNQQVEQSYYLLVPEPIDALGLRVVTCLSALTMPGRGDAV
                     TDDYTESCKSTCNFKAVSLNLTSSPDSSGKDRDLHSPLDSVTGNTSGMAWMQVQFGRL
                     PAGIYRISVRFEGANTPGGAACIQAGSSLPKRRLKLATSTELLLQSGE"
     gene            complement(782..>1941)
                     /locus_tag="P5292_10605"
     CDS             complement(782..>1941)
                     /locus_tag="P5292_10605"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRS39620.1"
                     /translation="ADLYSYIMTHDMSDPVHFDSVMAQLDGKSLVDYVLTNAISVCSD
                     WLNYNTGWWRGLDTSGTHRKWGYILWDNDATFGHYINYTGIPDITAYAAPCDPESLNG
                     PSDPEGHIQVLNRLRNNPVFEQFYIARMADLWNTVFSCGNMISQLDSITMMLTPEMPA
                     HCNRWSGDIADWQTNIAALRSYILTRCNNMAGGIRDCYSLNGPYIITLDADPVGAGSV
                     RLNSLVHTDLPWSGQYFGGMENILEASAISPYQFVNWTSPAQVFAPNDSAVLTSVNFT
                     SSDSIVAHFSFATNAFELKGESLTGSVYPIPAHDRAILEFRLPQSGPVRVSIFNSQGA
                     LVATPGEAQQLAAGFHSVQLDLANASLANGLYFVVLDTPSGTGTYRLPVER"
BASE COUNT          436 a          513 c          557 g          435 t
ORIGIN      
        1 gcagaattgt agtacttttt taaacatgtg gagtatggtt aatgggttta aatgtaggtg
       61 aaatgtgcta ggctcgtccg cgaaatggaa attaaaattg accctcaggc gattagacgg
      121 atgcaggaaa atcttagtaa attaggggta tgccggttcg ggtaggtgcc agtcttttag
      181 caattgtcgt gttcgtgttg tcgtgtttcc tgacgcaaca ccgggcaatg gcacaacggc
      241 tttctccgat ggctccgctg caaaatcacc cgttggagcg caaagctttt cagtcggaag
      301 tctctgttta tgattgcatc gacattcacg aaacggttaa ccagcaggtg gaacagtcct
      361 actacctgtt ggtgcctgaa ccgatcgatg cgctgggtct tcgggtcgtg acctgtttgt
      421 cggcgctaac gatgcccggt cgtggcgatg cagtgacgga tgactacacg gaatcgtgta
      481 aatcgacctg taacttcaaa gctgtaagtc tgaatcttac ttcgtcgccc gacagttccg
      541 gaaaggatcg ggatctccat tcaccacttg attccgttac cggcaatacc tccggtatgg
      601 catggatgca agtacaattc ggcaggttgc cggccggcat ctacaggatc tctgtccgct
      661 tcgaaggagc gaacacgcca ggcggcgccg cgtgcatcca ggccggcagt agtttgccaa
      721 agcgacgatt aaaactcgcc accagtacgg aactgctgct gcagtccggc gagtaggcga
      781 atcaacgttc cacaggcaat cgatacgtgc cggttccgga tggagtgtcc agtacaacga
      841 agtacaaccc attcgccagc gaagcgttgg caagatccag ttgcacggaa tgaaaccccg
      901 ctgcaagttg ctgcgcctct cccggggtag cgaccaatgc tccctggctg ttaaagatgc
      961 taacacgcac cggtcccgat tgcgggagcc tgaactccag tatcgcccga tcatgagcgg
     1021 gaatcggata gaccgaaccc gtcaacgact cgcccttcag ctcgaaggca ttcgtcgcaa
     1081 aactgaagtg cgcaacgata ctgtcgctgg acgtaaagtt cacgcttgtc agcacggcac
     1141 tgtcgttcgg agcaaacacc tgggcaggcg aggtccagtt cacgaattga taaggagaaa
     1201 tcgcgcttgc ctcaagtatg ttctccatgc cgccgaaata ttgtccgctc cacggcagat
     1261 cggtatgcac cagggaattc aaccgaaccg aacccgcacc aaccgggtca gcatcgagcg
     1321 tgatgatgta cggtccgttg agtgagtaac aatcccggat gccgcccgcc atgttgttgc
     1381 agcgcgtaag gatgtaactg cgcagtgccg cgatgttggt ctgccagtcg gcgatgtcgc
     1441 cgctccagcg gttgcaatgg gccggcatct cgggtgtcag catcatcgtg atgctgtcca
     1501 gttgggagat catgttgccg caactgaata cggtgttcca caaatccgcc atgcgcgcga
     1561 tatagaactg ctcgaatacg ggattgttcc ggagccggtt gagtacctgg atgtgaccct
     1621 ccggatccga cggtccgttg agcgattccg gatcgcaggg tgcggcatag gcggtgatat
     1681 ccggaatgcc ggtgtaattg atgtagtgcc cgaaggtggc gtcgttgtcc cagaggatgt
     1741 aaccccactt gcggtgcgtt ccgctcgtat cgagaccccg ccaccagccg gtgttgtagt
     1801 tgagccagtc ggaacagacg ctgatggcat tggtcagcac ataatcgacc aggctcttcc
     1861 cgtcgagctg cgccatgacg gaatcgaaat gcaccggatc gctcatgtcg tgggtcatga
     1921 tgtaactgta gagatcggcc c
//
LOCUS       DAOTON010000578         1941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_45819_length_1941_cov_3.224814, whole genome shotgun sequence.
ACCESSION   DAOTON010000578 DAOTON010000000
VERSION     DAOTON010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45819_length_1941_cov_3.224814"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..634)
                     /gene="nudK"
                     /locus_tag="P5292_10610"
     CDS             complement(56..634)
                     /gene="nudK"
                     /locus_tag="P5292_10610"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HRS39621.1"
                     /translation="MANIRILRSDVLSDNWYLLRNYTLEYTRSDGKVEVQKREVYDRG
                     NGVVVLLYNREQRTVVLTRQFRLPTSLNGNPDGRLIEACAGLLEDEHPDDCVRREVEE
                     ETGYQVRDVQKLMEVYMSPGVVTEILHFYFAEYHPERKVGKGGGHPDEQEDIEVLEMS
                     FERALAMIDDGRIRDAKTIILLQQALIKGLFR"
     gene            complement(643..774)
                     /locus_tag="P5292_10615"
     CDS             complement(643..774)
                     /locus_tag="P5292_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39622.1"
                     /translation="MASLGCAPVFARHYNAWALFDDRSVTSSPWAGLQRFGSTTRFP"
     gene            complement(796..1458)
                     /locus_tag="P5292_10620"
     CDS             complement(796..1458)
                     /locus_tag="P5292_10620"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HRS39623.1"
                     /translation="MNRLTRNFFFFCVLLFWLAAGACGSAFAQVKEEKPKYAPGKEKH
                     RNKTDEIGRKQGLWMFYNTFGEKIAETEFVNDIKEGIDRKFYSYDKVREETEYLGGVK
                     EGEYNRYYFSGQVQLEGTYKDRKKDGKWTKYFEDGSIRQEGSYKVGLRDGVWKTYNRK
                     GNVISESAYKNGIDLKVIEENKKKEDEAKKAAEKKKEGEKGKQPAPTTPPPATKPPQP
                     KK"
     gene            complement(1477..>1941)
                     /locus_tag="P5292_10625"
     CDS             complement(1477..>1941)
                     /locus_tag="P5292_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008991380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HRS39624.1"
                     /translation="MLREYFLTIGMAKVSTSAHEAFDLRILVDGRDEVVVNPDRVIGE
                     AKRAVLELADDGYTRPVERTDIKVLGRSALGMIYAGANAMESGHFISEHDRKISQKLA
                     WVLSGGDLSAPTLVSEKYLLDLEREAFLSLCGERKTLERIQSILTSGKPLRN"
BASE COUNT          350 a          533 c          550 g          508 t
ORIGIN      
        1 aatggtttga atggatggtt gacggccgga tccgccttgg attcggccgt ctttattacc
       61 gaaagagtcc ctttatcaac gcctgttgca acaggatgat ggtcttcgca tcgcggatac
      121 ggccgtcgtc tatcatcgcc aaggcccgtt caaacgacat ttcgagcact tcgatatctt
      181 cctgttcgtc cgggtgacct ccccccttgc ccacttttct ttccgggtgg tattcggcga
      241 aataaaaatg caggatctcg gtaacgacgc cgggcgacat gtacacttcc atgagctttt
      301 gtacatcccg gacctggtag ccggtctcct cctccacttc acgtcgcaca caatcgtcgg
      361 ggtgctcgtc ctccaggagt ccggcacagg cttcgatgag tctgccatcg gggttaccgt
      421 tgagtgaagt cggcaggcgg aattgcctgg tcaggacaac ggtgcgttgt tcccgattgt
      481 acagcagcac gaccacgcca ttgccgcgat cgtagacttc ccgcttctgc acttcgactt
      541 tgccgtcgct tcgggtgtat tcgagggtat aattccggag gaggtaccaa ttatcggaca
      601 gtacatcgga ccggagaatg cgaatgttcg ccatgggcag gctcagggga aacgtgttgt
      661 agaaccgaag cgttgcaagc cagcccaggg tgagctcgtc acgcttcggt catcgaacaa
      721 ggcccaggcg ttgtaatgac gagcgaacac gggagcgcat ccgagcgaag ccatccggtt
      781 ccgctccata ttactttact tcttcggttg tggaggcttc gttgccggcg gaggcgttgt
      841 cggagcgggt tgttttccct tctcgccttc cttcttcttc tcggcggctt tcttggcttc
      901 gtcttccttc tttttgttct cttcgatgac cttcaggtcg attccgttct tgtaggcgga
      961 ttcgctgatg acgttgccct tgcggttgta ggttttccag acgccgtcac ggagaccgac
     1021 tttgtaactt ccttcctggc ggatggagcc gtcttcgaag tacttggtcc atttcccgtc
     1081 tttctttcta tccttgtagg ttccttcgag ctgcacctgt ccggagaaat aataacggtt
     1141 gtactcccct tctttcactc caccgaggta ctcggtctct tcgcgcactt tgtcgtagct
     1201 gtagaatttg cggtcgatcc cttctttgat gtcgttgacg aattccgttt cggcgatctt
     1261 ctccccgaag gtgttgtaga acatccagag cccctgcttg cggccgatct cgtcggtctt
     1321 gttgcggtgc ttctcttttc ccggtgcata cttgggtttc tcttctttca cctgcgcgaa
     1381 ggcgctgcca caggcgcctg cggcgagcca gaagagcaag acgcagaaga aaaagaagtt
     1441 gcgggttagt cggttcatgc ggatggaaat gggtcgttag ttgcgcaagg gtttgccgga
     1501 ggtcaggatg ctttggatgc gctcgagcgt tttgcgttct ccacagaggg agaggaatgc
     1561 ttcgcgctcc aggtcgagga ggtacttttc ggagacaagg gtcggggctg aaagatcgcc
     1621 accggagaga acccaggcga gtttttgcga gatcttccga tcgtgttcgc tgatgaaatg
     1681 tcccgactcc atggcattgg caccggcata gatcatgccg agcgcggaac gaccgagcac
     1741 tttgatgtcg gttcgctcca ccggacgggt atagccatca tcggccagtt cgagtacggc
     1801 ccgcttggct tccccgatga cgcggtccgg attgacgacg acctcatcac ggccatcgac
     1861 cagtatgcgg agatcgaaag cctcgtgggc ggacgtggac actttcgcca ttccgatcgt
     1921 gaggaaatac tcgcggagca t
//
LOCUS       DAOTON010000579         1940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_45862_length_1940_cov_2.827586, whole genome shotgun sequence.
ACCESSION   DAOTON010000579 DAOTON010000000
VERSION     DAOTON010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45862_length_1940_cov_2.827586"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="P5292_10630"
     CDS             <1..894
                     /locus_tag="P5292_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS39625.1"
                     /translation="QAKLEKIKTDVEINTRDMENKETSTPLTAGLGFAGGLLIYLFIF
                     IYGAMVMRGVLEEKTSRIVEVVISSVRPFQLMMGKIIGVALVGLTQFLLWVVLTMIVY
                     AGITNVMLAGKSDQEKIETMMKVQPGMQQNPQMNEAMSEQTHEAGTKLREMMGSINFP
                     LLIGMFIFYFLGGYLLYSALFAAIGAAVDSETDTQQFMFPVTIPLILAYVAAINVLNN
                     PQGNIAFWFSIIPFTSPIVMMVRIPFGVPWEHVALSMVLLIGGFLFTTWLAGKIYRTG
                     ILMYGKKVSYKEMWKWLRYHN"
     gene            900..1826
                     /locus_tag="P5292_10635"
     CDS             900..1826
                     /locus_tag="P5292_10635"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRS39626.1"
                     /translation="MRVAVLDLGTNTFHLLVVDLQPDGSWSRIFKTRRVVRLGEGAIH
                     RNEIAPRPFARGLKTLLDFRKRIDTLAVEKVIAFATSAIRSAGNGKDFVQAAWKHTRI
                     RIRIISGEEEADYIAQGVRACVDWGDRVALIMDIGGGSTEFILADQRRVFWKSSFNIG
                     AARMLAHFNPSDPIRRSELAALKSYFRETLDPLAAAMKKHPPEVLIGSSGSFDTYAAM
                     LGLRHRGSDYLKGRKSHTFRLDEYRSLHRDLLKSSAREREQMPGLIRMRRDLIVMASI
                     CTDTVLELSGVGSMMVSRYALKEGMLASLSRK"
BASE COUNT          425 a          482 c          552 g          481 t
ORIGIN      
        1 caggcgaagc tggaaaagat caagacggac gtagagatca atacccggga tatggagaac
       61 aaggaaacca gtacgccgtt gacggccgga cttggtttcg cgggcggtct gctgatctac
      121 ttgttcattt tcatctatgg cgccatggtg atgcgtggag tcctggaaga aaagaccagc
      181 cgcatcgtag aggttgtgat ctcttcggtc cggcctttcc agttaatgat gggcaagatc
      241 ataggagtag ccctggtggg cttgacgcaa ttcctcctgt gggtcgtact gaccatgatc
      301 gtatacgccg gaatcaccaa tgtcatgctg gccggaaagt ccgatcagga gaagatcgaa
      361 acgatgatga aagtacagcc gggcatgcag cagaatccgc aaatgaacga ggccatgtcg
      421 gagcaaacgc acgaagccgg gacgaagttg cgggagatga tggggtcgat caattttcca
      481 ctgctgatcg gcatgtttat tttctatttc ttgggcggtt acctgttgta cagtgcgctc
      541 tttgcggcca ttggcgctgc ggtggactct gaaacagaca cccagcagtt catgtttcct
      601 gttaccatcc cgttgatatt ggcctacgtc gcggccatca acgtgctcaa caacccgcaa
      661 ggcaacatcg ctttttggtt ttcgattatt ccatttacat caccgatcgt catgatggtc
      721 cggattccct ttggcgttcc ctgggagcat gtggccctgt cgatggtact gttgatcgga
      781 ggttttttat tcaccacctg gctggccggc aagatctacc gtaccggtat cctgatgtat
      841 ggcaagaaag tctcctataa ggagatgtgg aagtggctcc gttaccataa ctgattccga
      901 tgcgggttgc ggtacttgac ctcggcacca acacgtttca cttgttggtg gtcgatttgc
      961 agcccgatgg ttcctggagc cgtattttca agacacgccg tgtagtccgt ttgggagaag
     1021 gagcaatcca tcgaaatgag attgctcccc ggccctttgc acgtggtttg aagacgttgc
     1081 tggattttcg aaagcggata gatacgctgg cggttgaaaa ggtcattgct ttcgccactt
     1141 cggcgatccg cagtgccgga aacgggaagg atttcgtgca ggcagcgtgg aagcatactc
     1201 ggatccgcat ccgtatcatc agcggtgagg aagaagcgga ttacattgcc caaggtgtac
     1261 gggcctgcgt ggattgggga gatcgtgttg cccttattat ggatattggc gggggctcga
     1321 cggaattcat actggccgat cagcgtcgcg ttttttggaa gtcaagtttt aatatcggtg
     1381 cagcccggat gttggcacat ttcaatcctt ccgacccgat ccgtcggtcc gaattggccg
     1441 ccctgaagag ctactttcgg gagacactgg atcccctggc tgccgccatg aagaagcatc
     1501 cacccgaagt gttgatcggc tcttccgggt cgttcgatac gtatgcggcg atgctgggcc
     1561 ttcgacatcg cggtagcgat tatttgaagg gccggaaaag tcatacgttc cgtttggatg
     1621 aataccggtc gcttcaccgt gacttgctca aatcctctgc cagggaaagg gagcagatgc
     1681 ccggcctcat ccgcatgcgc cgcgacctga tcgtgatggc aagtatttgc actgacacgg
     1741 tacttgaatt atcaggcgtc ggatcaatga tggtttcccg gtacgccctg aaggaaggta
     1801 tgctggcttc gctgagccgg aaatagtctc cctgcctgtt ccttcgtatc ttcgagaccg
     1861 gaaatcaaca gaccttatgc cgaagattct gatcatcgac gacgaaaagc cgatccggca
     1921 tacgctacgc gaaatcctcc
//
LOCUS       DAOTON010000580         1936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_45981_length_1936_cov_2.972355, whole genome shotgun sequence.
ACCESSION   DAOTON010000580 DAOTON010000000
VERSION     DAOTON010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45981_length_1936_cov_2.972355"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="P5292_10640"
     CDS             <1..289
                     /locus_tag="P5292_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRS39627.1"
                     /translation="RNSVLLFMVPCDSKDIRREYAILVNELEQYNPELLHKDRVLAIT
                     KCDLLDQELLQAMQSEVPKGVQAVFISSATGMGIDRLKDALWATLNKPEQP"
     gene            291..872
                     /locus_tag="P5292_10645"
     CDS             291..872
                     /locus_tag="P5292_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRS39628.1"
                     /translation="MWERLDAIDTQLLLALNGIHAPWLDSVFTFISARFVWLPLYAYF
                     AWKLYKALPGRFLVRLVVIALLITASDQLASGVIKPLVQRPRPCHVPELEGKLHLVDG
                     NCGGAYGFVSSHAANTFALATFLWLLFSRRHGRAGWYPMFAWAAVVSYSRIYLGVHYP
                     GDILCAALLGTVLGVLAYLVIVRIEKHPIAHAT"
     gene            862..>1936
                     /locus_tag="P5292_10650"
     CDS             862..>1936
                     /locus_tag="P5292_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRS39629.1"
                     /translation="MQRKFSFLRLTLLCSLLASFTTGRAQKSKVNPGGSSGPDRPRLV
                     VGIVVDQMRYDYLYRYWNQYGETGFKRLLREGFSCQDAHFDYVPTYTAPGHACIYTGT
                     TPSVNGIISNEWFDRESRKGVYCVNDTTVKPVGTTSISGKMSPHRLLTTTITDELRFA
                     TNDRSKVIALALKDRGAILPGGHRANAAYWHDPYLDNWVTSTYYMNALPDWVTRFNER
                     KVADSLLQYSWETLLPIREYTQSTEDDSPYEGKFKGETAPVFPHKLAELKKTDGELIR
                     RTPFGNTYTFMFAKAALAGEQLGKGEETDFLAVSLSSPDYIGHQFGINSIEVQDTYLR
                     LDRELGDFLSALDRQVGTGNYLVF"
BASE COUNT          437 a          494 c          516 g          489 t
ORIGIN      
        1 acgcaactcg gtattgcttt tcatggttcc ctgcgactcc aaagacatcc gcagggaata
       61 tgcgattctt gttaacgagt tggaacagta taacccggag ttgttgcata aggatcgggt
      121 actcgcaatc acgaagtgcg atctgttgga tcaggaatta ctgcaagcca tgcagtcgga
      181 agtgcctaag ggtgtacagg ccgtattcat ttcttctgct accggcatgg ggatcgatcg
      241 gttgaaggat gcactttggg cgaccttgaa caaaccggag cagccttgag atgtgggagc
      301 gactcgacgc gattgacacg caactgctgt tagccctcaa cgggatacat gccccctggc
      361 tcgattccgt ttttaccttc atcagcgctc ggtttgtctg gttgccgctc tacgcgtatt
      421 ttgcgtggaa gctatacaag gcattgcccg gacgatttct cgtccgcctg gtggtgatcg
      481 ctttgctgat cactgccagt gaccaattgg catccggcgt gatcaagccg ttggtacaac
      541 gtccgcgtcc ctgtcatgtt ccggagctgg aaggaaaact gcacctggtt gacggtaatt
      601 gcggaggcgc ttatgggttc gtgtcatcgc acgccgccaa taccttcgca ctcgcaacgt
      661 tcttgtggtt gttattctcg cgcagacacg gtcgtgccgg ttggtacccg atgtttgcct
      721 gggcagcggt agtttcctat agccgcattt atcttggcgt acattatccc ggggatatcc
      781 tatgcgcagc cttgctggga accgtccttg gcgtattggc ttatcttgtc atcgttagaa
      841 tagaaaagca ccccatcgct catgcaacgt aaattttcat tcctgagact taccctcctt
      901 tgcagtttgc tcgccagttt cacgactggc cgggcacaga aatcaaaagt caatcccggc
      961 ggaagcagcg gacccgaccg gcctcgtctg gtggttggta ttgtggtaga ccagatgcgt
     1021 tatgattatc tctatcggta ttggaatcaa tatggagaaa cgggcttcaa gcgattactg
     1081 cgggaaggat tttcctgcca ggacgcccat ttcgattacg tgccgaccta tacggctccc
     1141 ggtcatgcct gcatttatac cgggactacg ccctcggtaa acggtatcat ttcgaatgaa
     1201 tggttcgatc gcgagagcag gaaaggggtt tattgtgtca acgatacgac cgtaaaaccg
     1261 gtcgggacaa cctccatctc cgggaaaatg tcaccacatc gcctgctgac gaccacgatc
     1321 acggatgaac ttcgattcgc caccaacgat cgctccaagg tcattgcgtt agccctcaag
     1381 gatcgtggcg cgatattgcc cggcgggcac cgggccaatg ccgcatattg gcacgacccg
     1441 tatctcgaca actgggtgac cagtacgtat tacatgaacg ccttaccgga ttgggtgacg
     1501 cgtttcaacg aacggaaggt tgccgactcg ttgctgcaat attcgtggga aaccttattg
     1561 cccattcgtg aatacaccca aagtacagaa gacgattctc cgtacgaagg gaagttcaag
     1621 ggagaaactg ctccggtttt cccacacaaa ctggctgaat tgaaaaagac cgatggagaa
     1681 ttgatccgtc gcacgccatt cggaaatacg tacacgttca tgttcgcgaa ggccgctttg
     1741 gcgggtgagc aattgggaaa aggagaggag actgattttt tagcagtcag tttatcttct
     1801 cctgattata tcgggcatca atttggtatc aattcgattg aagtacagga tacctatttg
     1861 cgactggacc gcgaactggg agatttcctg agcgcgcttg accgtcaggt cggcaccgga
     1921 aactatctcg tgttcc
//
LOCUS       DAOTON010000581         1933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_46087_length_1933_cov_2.668797, whole genome shotgun sequence.
ACCESSION   DAOTON010000581 DAOTON010000000
VERSION     DAOTON010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46087_length_1933_cov_2.668797"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="P5292_10655"
     CDS             <1..271
                     /locus_tag="P5292_10655"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRS39630.1"
                     /translation="ILRCESDGNYTTVHALEGKPILICRTMKEFEDLLVDHTQFFRIH
                     RSHIINMRFVKEILKNDGLVRMRDGKELEISRRRREEFYDAFMRL"
     gene            554..1417
                     /locus_tag="P5292_10660"
     CDS             554..1417
                     /locus_tag="P5292_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39631.1"
                     /translation="MKNSILRLSVLLAFLYSCSGNTSSNSTESGSSPQALMKSYAGAA
                     ANSAPGYETCNTYPGSNHRICYRTLLRLTQAFIRKYSEDATLNGLGGYLDKESLLSAM
                     NADASYNGIVFTTAYYNGTFKNESYLAYQGMKVEDSKEIPCELQKTDYYPVSTSQFIY
                     TETDTTLSAISDFVAQEPEPIEPMTTFQAGNNLETNAGTFLSHFLSAGSAANQRSCFF
                     FGKTELEALLNQEGCVGMRFLWGFEEERSSDKVRILLIGVDDEGNNLLVKPGTSSDEA
                     IYMERGWPPAD"
     gene            1420..>1933
                     /locus_tag="P5292_10665"
     CDS             1420..>1933
                     /locus_tag="P5292_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39632.1"
                     /translation="MDTEHLRFFFGHMAMAITAMVLLFSIFRYRNFNVFDWQMWAFLF
                     YGLITDTIARLSFSFPPRAYFVINVFNLFESLFFLLFARQFVQSEKLKKLLVILAALS
                     LLAWTWFQFNPIDGVTIHLKMHPTFGSIYCILSTFLYAYLLLRLIESRSNVLRQSTFW
                     YVLGIFFSSFT"
BASE COUNT          436 a          528 c          474 g          495 t
ORIGIN      
        1 gatcctccgc tgcgaatcgg acggtaacta cacgacggtt cacgcgctgg aaggaaagcc
       61 gattctcatc tgccggacca tgaaagagtt cgaggacctg ttggttgacc acacccagtt
      121 ctttcgcatt catcggtcgc acatcatcaa catgcgcttc gtgaaagaga tactcaagaa
      181 cgacggtctg gtgagaatgc gtgacggaaa ggagctggaa atttcgagac gtcgtcgcga
      241 agagttttat gacgccttta tgcgcctctg agtcccgcca ttcactcgtc cggttcggcc
      301 attgatggtc gaagtccaac cgcttgctca gtcggaccgg tcagttcata cgcttccact
      361 agttttatcc atggaggagc cttttcggct ttcatgggac cttttcggcg ctaacgcgca
      421 ggatcagttt atttttcgca cgacctcaac aatttcggaa gcgccaagct gccggttgac
      481 gaagtgatgg gtagtccggg accggcagct cttccgataa acttgctcgt gtatcaccaa
      541 tccacaacca accatgaaaa acagtatcct gcgcctctct gttctgcttg ccttcctgta
      601 ttcctgcagc ggcaacacat cttccaactc caccgaaagc ggttccagtc cgcaagccct
      661 gatgaaatcg tacgcaggcg ccgccgcgaa ctccgcgccc ggttatgaaa cctgtaacac
      721 ctatcccggt tccaatcacc ggatctgcta ccggaccctg ctgcgtctga cacaggcgtt
      781 catccggaag tattcggaag acgccacctt aaacggactc ggcggatacc tcgacaagga
      841 gtcgttgttg tctgcaatga atgcggacgc gtcgtacaac gggatcgtct tcaccaccgc
      901 gtattacaac ggaacgttca agaatgaatc ctacctcgcc taccagggca tgaaagtcga
      961 ggacagcaag gagatcccct gcgaattgca gaagacggac tattatccag tcagcacctc
     1021 gcaattcatc tatacggaaa ccgatacgac actttcggct atttccgact tcgtggcaca
     1081 ggaaccggag ccgatcgaac ccatgacaac tttccaggca ggcaataacc tggagaccaa
     1141 tgccggcacc tttctttcgc actttctctc ggcgggatcc gccgccaacc agcgaagctg
     1201 tttctttttc ggaaagacgg aactggaggc cttgttgaat caggaaggtt gcgtgggcat
     1261 gcgttttcta tggggctttg aagaggaaag gagcagtgat aaagtgcgta tcttgctcat
     1321 cggggtggat gacgagggga acaacctgct cgtcaagcca ggtaccagca gtgatgaggc
     1381 gatctacatg gaacgtggat ggccgcctgc agattaagca tggatacaga acatctgcgt
     1441 ttcttcttcg gacacatggc catggcgatc accgccatgg tcttgctttt ttccatcttc
     1501 cgctaccgga atttcaatgt ttttgattgg caaatgtggg cgttcctgtt ttatggtctt
     1561 ataaccgata ccattgcaag actctcgttc tcttttcctc cccgggcgta tttcgtgatc
     1621 aatgtgttca acctgttcga gagcctgttt tttctgctct ttgcccgaca gtttgttcaa
     1681 tcagaaaagt tgaagaaact actcgtcata ttagcggccc ttagcctatt ggcctggacg
     1741 tggtttcaat tcaaccccat cgatggggtc acgatccact tgaagatgca tccgactttt
     1801 ggcagcatct attgtatcct gtcaaccttc ctctatgcgt acctgttgct tcgactgatt
     1861 gaaagccggt ccaatgttct ccggcaatcc accttctggt acgtgttggg tatttttttc
     1921 tccagtttca cgg
//
LOCUS       DAOTON010000582         1931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_46148_length_1931_cov_3.348614, whole genome shotgun sequence.
ACCESSION   DAOTON010000582 DAOTON010000000
VERSION     DAOTON010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46148_length_1931_cov_3.348614"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="P5292_10670"
     CDS             <1..244
                     /locus_tag="P5292_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HRS39633.1"
                     /translation="FKKEIFENVTLASRLELFNNYTDEDENNRKNIDVNWETGLLMKV
                     NKFLTTSFIATVVYDHNVIQRTQYRQILGVGFGYKF"
     gene            308..751
                     /gene="crcB"
                     /locus_tag="P5292_10675"
     CDS             308..751
                     /gene="crcB"
                     /locus_tag="P5292_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRS39634.1"
                     /translation="MGTGKHRSSNYYLGLAILPDYRMNNVLLLALGGAMGTIARYGTG
                     LWINRTLEQPLFPWGTFAINLTGCLVIGLLAGMNERNPFEVSARLFLFTGLLGGFTTF
                     SAFGLESVQLIRNGHWQLAGLYAGSSTLVGILFAYAGYRLTMGGR"
     gene            851..>1931
                     /locus_tag="P5292_10680"
     CDS             851..>1931
                     /locus_tag="P5292_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39635.1"
                     /translation="MRSPFLLVYLACCLLSLPRSTGQLFRPQARFSDNAAGVTVMARH
                     PEGKMVINGDRDGNLYFRDASTGQVFRKIKAHQAPVNTLSFNSNGQLLISSTFDGEIR
                     IYDFQKDNFVQSIYSPDYAGIRFVLFSIADGFIYFNSNSRLYKTRSDLTQKVDLIRQE
                     DDTLYDAVIAADRSAVIYSKGHSLHVLNTRSDLLRQEFSPGPFPIRYMNFLNDTTLVT
                     WSDDGTLAFWSFRMGQLALNPTSWMKAGLPCRMAFSSDGKFMATGMVGNWARVWSPQE
                     RAIFQELFGHQQTVTATCFGKNDHILFTGSNDRSVRVWSREENKTSPPDTNPIPPPLV
                     VDTTPLTVKPPEIDTVHVAQHEQVKA"
BASE COUNT          436 a          549 c          480 g          466 t
ORIGIN      
        1 cttcaagaag gagatcttcg agaatgtgac ccttgcttcc cgtctcgaac tattcaacaa
       61 ctacaccgat gaggacgaga ataaccggaa gaacatcgac gtcaactggg aaaccggctt
      121 attgatgaaa gtgaacaaat tcctcacgac ctccttcatc gcaaccgtcg tgtacgacca
      181 caacgtcatc caacggaccc aataccgtca gatcctgggc gtaggcttcg gttacaaatt
      241 ctaatcagta ccctgatctt tttaaaaccc cggcaactca ccggggtttt gcattttata
      301 acctcatgtg ggcactggta aacatcgttc gtcaaactat taccttggcc ttgccatctt
      361 gcccgattat cgcatgaaca atgtcctgct gttagccctg ggtggtgcca tgggcactat
      421 cgcccgctat ggtaccggtt tgtggatcaa ccggacgctt gagcaaccac tcttcccctg
      481 gggaaccttt gccatcaacc tcaccggctg cctggtcatt ggcttgttgg ccggcatgaa
      541 cgaacgcaat ccttttgagg tatcggcgcg attgttcctc ttcaccggct tactgggagg
      601 attcactacg ttctccgcat tcgggttgga atccgttcaa ctgatccgga acggtcactg
      661 gcaactggcc gggctttatg ccggctcctc taccttagta gggatcttgt tcgcgtatgc
      721 gggctaccgg ctgacgatgg gcgggcgatg aaccattgtg tttgattagg gaattgaaca
      781 tccgattgtg ataattccgt tcacattgcc tcctggaatg caatagtccc cggtaacttt
      841 acgggattgc atgagatcac cgtttctgct cgtttacctt gcttgctgcc tgctgagcct
      901 gccccgatcc accgggcagt tgttccgacc gcaagccagg ttttcggata atgccgctgg
      961 tgtgaccgtc atggcgcgtc acccggaagg aaagatggtg atcaacggtg accgggatgg
     1021 caacctctat ttccgggatg caagcacggg acaggttttt cgtaagatca aagcccatca
     1081 ggcgcctgtc aacacgcttt cgttcaattc gaacggacaa ctcctgatca gctcgacctt
     1141 cgacggagag atccgcatct acgattttca gaaggacaat ttcgttcagt cgatctactc
     1201 tccggattac gccggtatcc ggtttgtcct gttcagcatc gcggatggtt tcatctattt
     1261 caacagcaac agccgactat acaagacacg gtccgatctt acccaaaagg tggatctgat
     1321 ccgccaggag gacgacacct tgtacgacgc ggtgatcgct gccgatcgca gcgcggtcat
     1381 ttattccaaa ggccatagcc tccacgtgct gaacacacga tcggatctct tacggcagga
     1441 attcagccca gggccttttc caatccggta catgaatttc ctcaacgaca ccaccctggt
     1501 cacctggagt gacgacggca cactggcatt ttggagtttc cggatggggc aacttgccct
     1561 gaatccgact tcctggatga aagccggact accctgcagg atggccttct cctccgatgg
     1621 caaattcatg gcgacgggca tggtaggcaa ttgggcgagg gtctggtcac cccaggaacg
     1681 tgcgatcttt caggaacttt tcggtcacca gcaaacggtg actgcaacct gttttggcaa
     1741 gaacgatcat atcctgttca ccgggagcaa cgaccggtcc gtgcgtgtct ggtcacgtga
     1801 agaaaacaag accagtccac ccgacaccaa tccgattccg ccgccccttg ttgtcgatac
     1861 tacgccgctt acagtcaagc cgcccgaaat cgacactgtt catgttgcac aacacgagca
     1921 ggtgaaagcc g
//
LOCUS       DAOTON010000583         1927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_46297_length_1927_cov_2.220085, whole genome shotgun sequence.
ACCESSION   DAOTON010000583 DAOTON010000000
VERSION     DAOTON010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46297_length_1927_cov_2.220085"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..994)
                     /locus_tag="P5292_10685"
     CDS             complement(8..994)
                     /locus_tag="P5292_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRS39636.1"
                     /translation="MRHHDGPGVDHAEVIRSALFGVAVGDALGVPVEFTNRARLSKDP
                     VTDMIGHGTYDLPAGTWSDDSSLTFCLAEALTQEFDLDTIGRNFVKWYHEGYWTPYGN
                     VFDIGITTREAINRIPRGAKAGSAGGTDENANGNGSLMRILPLVFHLANRPVDERFRI
                     TQQVSSITHGHIRSAIACFYYLEFAKRIMEGEDKFGIHRDLGTEVLDHLTARSTDPME
                     IAVFDRLLKGDIHHLDQDRIESGGYVMHTLEASIWCLLTTDNFKEAVLKAVNLGRDTD
                     TTGAVTGGLAGLLYGMKNIPENWLRKLARYDDIQDLVARLAVKLPGHQRTGR"
     gene            complement(981..1433)
                     /locus_tag="P5292_10690"
     CDS             complement(981..1433)
                     /locus_tag="P5292_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39637.1"
                     /translation="MGLPGAIRCYVQMHDPASMICYETSIFSDEETYQAVIRVLEGHS
                     NIQGAAAEGATIHFGYCYGGMGNHVFIKSLCDLERKDGYFIYRMGDLRFRISPSARGV
                     WDRAALWLSMRRFIVRVRDKKNPPEWIDGPAGSARGYIDPMMLGNEAP"
     gene            complement(1658..>1927)
                     /locus_tag="P5292_10695"
     CDS             complement(1658..>1927)
                     /locus_tag="P5292_10695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRS39638.1"
                     /translation="VRPEFQKRGIGGQLIKQGLEVAEGSDFKSVIVLGHEHYYPKFGF
                     EPADKWNIKAPFDVPSDVFLAIELVKDGLKNISGTVIYPKEFGTV"
BASE COUNT          389 a          582 c          482 g          474 t
ORIGIN      
        1 cgcgcagtta ccggccggtt cgttggtggc cgggaagttt gaccgccagt cgtgccacca
       61 ggtcctggat gtcgtcatac ctggccaatt ttcgtaacca gttttcggga atgttcttca
      121 tgccgtacag cagaccggca aggccgccgg tgaccgcacc tgtcgtgtcc gtgtccctgc
      181 ccagattgac cgccttcagg acggcttcct tgaaattgtc agtggtcagc aaacaccaga
      241 tactggcctc cagcgtatgc atcacatagc cgccgctttc gatccggtcc tggtcgaggt
      301 ggtggatgtc gcctttcaac aaccggtcga acacggcgat ctccatggga tccgtggagc
      361 gtgccgtcaa gtggtcgagc acctcggttc ccagatccct gtggatcccg aacttgtcct
      421 cgccctccat gatccgcttc gcgaattcga ggtagtagaa gcacgcgatc gcggaccgga
      481 tgtgcccgtg ggtgatcgac gagacctgtt gggtgatccg gaagcgttcg tcgaccggcc
      541 tattggcaag atggaacacc aacggcagga tccgcatcaa cgaaccgttc ccattcgcgt
      601 tctcgtccgt gccgcctgcc gatcccgctt tcgcccctct tgggatccgg ttgatggcct
      661 ctcgtgtcgt gataccgata tcgaagacat ttccgtacgg tgtccaatag ccctcatgat
      721 accatttcac gaaattccgt ccgatcgtgt cgaggtcgaa ttcctgcgtc aatgcttccg
      781 cgagacagaa ggtcagcgaa ctgtcgtccg accaggtccc tgcgggcagg tcgtacgtcc
      841 cgtgaccgat catatccgtt acagggtcct tgctcagcct tgccctgttc gtgaattcga
      901 ccggaacacc cagcgcatcg cccaccgcaa ctccgaaaag tgcggacctg atcacttcgg
      961 cgtgatcaac gcccggtcca tcatggtgcc tcattgccca gcatcattgg atcgatgtac
     1021 ccgcgtgcgc tccctgcagg cccatcgatc cattccggag ggttcttctt gtccctcact
     1081 ctgacaatga atctacgcat cgacaaccaa agagccgccc ggtcccagac acctcgagcg
     1141 gaaggagata tccggaaccg aaggtcgccc attctgtaaa tgaagtagcc gtcctttctc
     1201 tccagatcgc acaagctttt gatgaagacg tgattgccca tgcctccata acaatagccg
     1261 aaatgtatcg tcgcgccctc agcggccgca ccctggatgt tcgaatgccc ttccaacacc
     1321 ctgatcacag cctggtaggt ctcttcatcc gagaagatgc tggtctcgta gcatatcatg
     1381 ctggccggat cgtgcatctg gacatagcag cggatggcgc ctgggagccc catcgccccc
     1441 ggccatgcga catccgccta gatcacgtca tccaggacga tatcggccag gaccgatgcc
     1501 gtcagatctt ccacgatggg ctcaatcacg gttccgtcgc tctgttccat ggtcgatcga
     1561 tggttggcgt gttggtgttt gtgccgaatt aatgaatttt cgtctcgtgg catgaatgat
     1621 gtcaggcctt cgcccgtctt ctatcgatct tgttgtgtca tactgttcca aattctttcg
     1681 gataaataac agtccccgat atatttttca gtccgtcttt taccaactcg atcgccaaaa
     1741 aaacatcaga aggtacatca aacggtgctt tgatattcca cttgtcggct ggttcaaatc
     1801 cgaatttcgg gtaataatgt tcgtgtccca aaacgatcac cgacttaaag tctgatcctt
     1861 ctgctacttc aagtccttgt ttgatcagtt gtccgcctat tccacgtttt tgaaattcag
     1921 gtctaac
//
LOCUS       DAOTON010000584         1924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_46385_length_1924_cov_2.487961, whole genome shotgun sequence.
ACCESSION   DAOTON010000584 DAOTON010000000
VERSION     DAOTON010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46385_length_1924_cov_2.487961"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="P5292_10700"
     CDS             <1..1324
                     /locus_tag="P5292_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS39639.1"
                     /translation="SGGNYTVTVSDANGCVNLYQFEIEEPAPLLAALPADQALCPGFV
                     LVLATSVSGGTQPYTYLWTDGSTDSSQTVSPVGTTQYGVTVTDANGCSSGPLSVTISV
                     PDPLTVVATGDATLCDGSATTIGSQASGGAGTYTYSWNSGQILTPGSTVYPAQDTTFT
                     VTVTDACGTTATDQVTVLVRPAPEVAFTPQEIAGCSPVVVNFLDGSTTPAGSTYAWNL
                     GDGSTSSETSPIHTYTEPGTYSVSLLIETPDGCIDYLNVSNLVTVHPYPVAGFSASAD
                     TVSLVNANVSFENTSSGASWYAWDFGDGSPVEHGSSAAHNYADTGTYQIQLIVMNPAG
                     CLDTIFGTIRVEQDFAIYIPNAFTPNKDGVNDGFLPLGVGWTDFEMWIMDRWGLVIYH
                     STSPETPWNGSYQNNEQPCQQDVYEYIVSVKDFRGKGHRYVGHVTLVR"
     gene            complement(1395..>1924)
                     /locus_tag="P5292_10705"
     CDS             complement(1395..>1924)
                     /locus_tag="P5292_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRS39640.1"
                     /translation="PPFVVFQDPSLEEMAIQYPITMDELKNISGVGAGKATKFGKPFV
                     ELIKKYVEENEIDRPMDLVVKSIVNKSGLKVYLIQNIDRKISLNDMATAKGMTLENLL
                     TELETIVSSGTRIDINYYINEVVDIDRQAEVFEYFKSADSDSAEEALRELGENDYSIN
                     DIRLMRIKFLSELGN"
BASE COUNT          445 a          526 c          499 g          454 t
ORIGIN      
        1 gagcgggggc aattacaccg ttaccgtatc ggatgccaat ggttgtgtga acctctacca
       61 atttgaaatc gaagaaccag ctcctctgct ggctgctcta ccggctgatc aggcgctgtg
      121 tcctggcttt gttttggtgt tggcgacttc cgtttccgga ggtacccaac cgtacactta
      181 tctctggacc gatggcagca cggactcttc acaaaccgtt tcgcccgtcg gtaccacgca
      241 atacggcgtt acggtcaccg atgcgaacgg atgttcctca ggacccttgt cggtcaccat
      301 cagtgtcccc gacccgctga cggtggtagc aaccggagat gccaccttat gcgatggcag
      361 cgcaaccacg atcggatcac aggctagtgg tggtgcggga acctatacgt atagttggaa
      421 tagcggtcag attttgaccc ccggcagtac agtttaccca gctcaggaca ccaccttcac
      481 cgttaccgta accgatgcct gtggcactac ggctaccgat caggttaccg tgctggtcag
      541 accggctccc gaagtcgctt tcacccctca ggaaattgcg ggttgcagcc cggtggtggt
      601 aaacttcctg gatggatcca cgacccctgc aggatcaacc tatgcctgga acctgggcga
      661 tggatccacg agcagcgaaa cgtcaccgat tcatacctat accgaaccag gaacctacag
      721 cgtaagtctg ctgatagaaa caccggatgg ttgtatcgat tatctgaacg tcagtaatct
      781 ggtcaccgtg catccctatc cggtagccgg tttctccgcc tccgctgata cggtatcact
      841 cgtgaatgcc aacgtatcat tcgagaacac cagtagcgga gcaagctggt acgcctggga
      901 tttcggcgac gggtctccgg ttgaacacgg atcatcggcg gcgcacaatt acgcggacac
      961 cggcacctat cagatccaac tgatcgtcat gaatccggcc ggttgcctgg acaccatttt
     1021 cggcaccatc cgggtagaac aggatttcgc gatctatatt ccgaacgcct tcacgccgaa
     1081 taaagatggt gtgaacgacg gcttcctgcc actgggtgta ggatggacag atttcgaaat
     1141 gtggatcatg gaccgctggg ggctggtcat ctaccattcc accagtccgg aaacaccctg
     1201 gaacggttct taccaaaaca acgaacagcc ttgccagcag gatgtatatg aatacatcgt
     1261 cagtgtgaag gacttccggg gtaaaggaca ccgctacgtt gggcatgtaa ccctggtgcg
     1321 ctgatagcgg aacacataaa taaaaatggc tgccggtggg cagccatttt tatttaaggc
     1381 aatccggtcg ggaatcagtt gccgagttcc gagagaaact tgatacgcat caaacggata
     1441 tcgttgatgg aataatcatt ttcaccgagt tcccgcagcg cttcttcggc ggagtcggaa
     1501 tcagccgact tgaagtattc gaacacttca gcctggcggt caatgtcgac gacctcgttg
     1561 atgtaataat tgatgtcgat ccgggttccc gaagagacga tcgtttcaag ctcggtcaac
     1621 aggttttcca gggtcatgcc cttagccgtt gccatatcgt tgagtgaaat cttccggtcg
     1681 atgttttgga tcaggtagac tttcaagccc gacttgttca caatcgattt gaccacgaga
     1741 tccattggac gatcgatctc attctcctcc acatacttct tgatcagttc gacgaatggc
     1801 ttcccgaatt tggtcgcctt ccctgctccg acgccggaaa tgttcttcag ttcatccatg
     1861 gtgatcggat actgaatggc catttcctcc agcgaagggt cctgaaagac cacaaagggt
     1921 ggaa
//
LOCUS       DAOTON010000585         1923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_46419_length_1923_cov_3.145610, whole genome shotgun sequence.
ACCESSION   DAOTON010000585 DAOTON010000000
VERSION     DAOTON010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46419_length_1923_cov_3.145610"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="P5292_10710"
     CDS             <1..344
                     /locus_tag="P5292_10710"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRS39641.1"
                     /translation="EEVGIHPDTIELLGRLTPLYIPPSGFLVHPFVGWQGRSPEFAID
                     PAEVASLFEVSVSRLLDPNAISEERIRLQSGLQLTTPAFRFDDRIVWGATAMILSEFG
                     EVWRQALDPTQ"
     gene            complement(391..1710)
                     /locus_tag="P5292_10715"
     CDS             complement(391..1710)
                     /locus_tag="P5292_10715"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase, eukaryotic"
                     /protein_id="HRS39642.1"
                     /translation="MKTDKLKEKFAKVALPMAAELKDFIKENGNHVIGSYTIEQVYSG
                     MKGMIGMVTETSKLDPEEGIRFRGYSIPELREKLPKAPGDTEPLPEGIFYLMLIGEIP
                     TDEEATYVSNQWARRSNVPKHVFDMLDKLPANTHPMTQFSMAVMALQTESVFASAYRK
                     GLSKKEYWDPMYEDVMNLIARLPRVAAYIYRRVYKNGVHIEPDPKLDWAANFAHMLGY
                     DDYNMKRLMRLYLTIHADHEGGNVSAHSTHLVGSALSDAYLSFAAGMNGLAGPLHGLA
                     NQEVVRWLIDLRNYYDGATPSKEQIHEYVMKTIEDGKVIPGYGHAVLRKTDPRFIAQM
                     EFAKTYMPDDSMCQIVHTVYDVVPPILSGIKKIKNPWPNVDAHSGALLIHYGMEEYDF
                     YTVLFGVSRALGVLASLLWDRALGHPIERPGSVTTEWMKAQAMKAHA"
BASE COUNT          406 a          524 c          501 g          492 t
ORIGIN      
        1 atgaggaagt cggcattcat cccgatacga tcgaactgct tggccgcttg acgccactct
       61 acattcctcc cagcggtttc ctggtgcacc ccttcgtggg ctggcagggc cgatcgcctg
      121 aattcgccat cgaccctgcc gaggtcgcgt ccctttttga agtttccgtt tcccggctct
      181 tagatcccaa tgctatttcc gaggagcgca tccggttgca aagtggtttg cagctcacca
      241 ctcctgcctt ccgcttcgat gaccgtattg tctggggtgc tacggcgatg atcctaagcg
      301 aattcggaga agtctggagg caagccctgg atccgacaca ataaaaaagc cccgctgaag
      361 cgaggctttt ttatttgaga taagtcaaga tcaggcgtgt gccttcattg cctgggcttt
      421 catccattcg gtcgtgacgg agcccggtcg ttcgatcgga tgtccgagcg cacgatccca
      481 caagagcgac gcgagtaccc ccaaggcgcg gctgacgccg aagagcaccg tatagaaatc
      541 atactcttcc atgccgtagt gaatgagcaa tgccccactg tgggcatcca cattcggcca
      601 ggggttcttg atctttttta ttccgctcag aatcggaggc accacatcat agaccgtatg
      661 cacgatctga cacatggagt cgtcgggcat gtaagtcttg gcgaattcca tctgggcgat
      721 gaagcgcgga tcggtcttgc gcagtacggc atggccgtag cccggaatca ctttcccatc
      781 ctcgatcgtc ttcatgacat attcgtggat ctgttctttg gaaggcgtcg cgccgtcgta
      841 ataattccga aggtcgatga gccagcgaac cacttcctga ttggccagtc cgtgcaaagg
      901 gcccgccagt ccgttcatac cggccgcgaa ggacaggtaa gcatcactca gagccgaacc
      961 gacgaggtgg gtggaatggg cggagacgtt tcctccctcg tgatcggcat ggatcgtgag
     1021 gtagaggcgc atcaggcgtt tcatgttgta atcgtcgtac cccaacatgt gggcgaaatt
     1081 cgcagcccaa tccagtttgg gatccggttc aatatgaacg ccattcttat agacccgacg
     1141 atagatgtac gcggccacgc gcggcaaacg ggcgatgaga ttcatcacat cctcgtacat
     1201 cggatcccaa tactcttttt tgctgaggcc cttccggtaa gcgctggcga aaaccgattc
     1261 agtttgcagc gccatgacgg ccatgctgaa ctgggtcatc ggatgggtgt tagccgggag
     1321 tttatccagc atgtcgaaca catgcttggg aacgttgctc cggcgcgccc actggttgga
     1381 cacgtaggtc gcttcttcat ccgtcgggat ctcaccgatc agcatgaggt agaagatacc
     1441 ttccggtaac ggttccgtat caccgggagc cttgggcagt ttttcccgca gttcaggtat
     1501 gctgtaaccg cgaaaacgga taccttcttc cggatcaagt ttacttgttt cggtcaccat
     1561 cccgatcatt cccttcattc cgctatagac ctgttcgatc gtataggagc cgatgacatg
     1621 gtttccgttt tccttgatga aatctttcag ctccgctgcc attggcaacg ccacttttgc
     1681 gaatttctct ttcagcttgt cggttttcac tgcagtaaga ttaaaaggcc gcgagggtcg
     1741 gacttgttat tggttgttgt atcgttcctg agtctttcgg aacttttcct gatttgcctt
     1801 cttattagag ctctcgatat tcaattccgg gaatacgatg atgagactga ccagaaacgc
     1861 aaggataagg aatacccaca atgccatctg accatacttt ttcatccgtc gctttgagat
     1921 caa
//
LOCUS       DAOTON010000586         1923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_46422_length_1923_cov_2.902570, whole genome shotgun sequence.
ACCESSION   DAOTON010000586 DAOTON010000000
VERSION     DAOTON010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46422_length_1923_cov_2.902570"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /locus_tag="P5292_10720"
     CDS             complement(<1..1522)
                     /locus_tag="P5292_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HRS39643.1"
                     /translation="MTVSNQSTVSSFIANGSATVFNFNFQVLDSRDLKVHVNGNQKVV
                     GYDYTVSGIGNSYGGNVNFLSPPEENSVVVIYRESSLSRETDYQRNGDLRAETINYDF
                     DRLWLVMQEAFVKFNRAPLLPIDSQISSTIDFPKPESGKYIRWNLNGTALEAVDGTGT
                     SEAYFLQSGSGAVSRSMQSKIGDFVNVRDFGALGNGSANDTAALQAAIDYAESLGGGT
                     VHFPVGTYLISATLTVQKGVRLVGEGQIQDSTSSSGAAEGSCIRWNSASHLDMCQIKS
                     ATFGNTLRGAGSINIVWYGSSYAKCAIRASSVMHCEFDGVSLAFTEAGLVLDNGNGVL
                     CGLTKIPRWEHIYGSNAATEGSHGLVLDGDVMPDPTSGVTNNLIGSVTGLIKNGNLVH
                     MRDCDGNVIQHIQASRVSGGTGRAIAFKNGSQRHARNNMVVGMLGSVLAESSTRGNNI
                     LHLHSEAADLQIDTGGQLHYNTTDYISADQWQTHRYVMSDQLLLPPGVFSTEEEFVA"
     gene            complement(1553..>1923)
                     /locus_tag="P5292_10725"
     CDS             complement(1553..>1923)
                     /locus_tag="P5292_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRS39644.1"
                     /translation="ETNGPGGFVPNILRQALKGTGCGVGEEFSVTNKQKRILDAFEAP
                     MSSRFLWAHVSVLEGPVWDQLKDFNPDLRDQPDDYLDSGAGAIAQTPIRIGKKVGNPT
                     ELSREDWRPSAGVYEVAFEH"
BASE COUNT          438 a          516 c          472 g          497 t
ORIGIN      
        1 aagcgacgaa ttcttcttcc gtagaaaata caccgggtgg gagcaaaagc tgatcgctca
       61 tcacgtaacg atgcgtctgc cattgatcgg cgctgatata gtctgtcgtg ttgtagtgca
      121 gttgcccgcc ggtatcgatt tgcaaatcgg ctgcttcgga gtgcaggtgg aggatattat
      181 ttccacgcgt gctgctttca gccagtacgc ttccaagcat cccaacaacc atattgttcc
      241 tcgcatgccg ctgcgagccg ttcttgaagg cgattgctct ccctgtgccg ccactgacgc
      301 gtgatgcctg aatatgctgg atgacgtttc cgtcgcaatc ccgcatgtga acaaggtttc
      361 cgttttttat tagaccggta actgaaccga tcaggttgtt agtcacgcca gaagtcgggt
      421 ctggcatgac atccccatcg agcaccagtc cgtgcgatcc ttccgtagcc gcgttagacc
      481 cgtaaatatg ctcccaacga ggtatttttg tcagaccgca aagcacgccg ttgccgttat
      541 caagtaccaa gcctgcttcg gtgaatgcca gagaaacgcc gtcgaattcg cagtgcatga
      601 ccgacgatgc gcggatagcg cactttgcgt agctgctgcc gtaccagacg atatttatcg
      661 acccggcccc tcgaagggta ttgccgaacg tcgccgattt aatctgacac atgtccaggt
      721 gggatgcgct gttccaccgg atgcaagatc cttccgccgc accgctgctc gacgttgaat
      781 cctgaatttg gccttcaccg acaagacgca cccccttctg caccgtcaga gtggcagaaa
      841 tcagataggt gccaaccggg aagtggactg ttcctccgcc aagactttcg gcgtagtcga
      901 tggcggcttg caaagcggct gtgtcgtttg cagacccgtt accgagcgct ccaaaatccc
      961 tgacgtttac aaagtctcca atcttgctct gcatagatcg agagacagca cctgatccgg
     1021 actgcaaaaa ataggcttct gaagtccctg ttccatcaac tgcttcaaga gcagttccat
     1081 tcaagttcca cctgatatat tttccggatt caggttttgg aaagtctata gtggaagaaa
     1141 tttgagaatc gatagggagt agcggtgcgc gattaaattt tacgaatgcc tcctgcataa
     1201 caagccataa tcgatcaaag tcgtaattta tcgtttctgc gcgcagatcc ccgtttcttt
     1261 ggtagtctgt ttctctgctt aaagaagact ctctgtaaat aacaaccact gaattttctt
     1321 caggtggtga taaaaaattc acatttccgc catatgaatt tcctattcca gataccgtgt
     1381 agtcatatcc gacaaccttt tgatttccat taacatgaac tttcaaatct cttgaatcaa
     1441 gaacctggaa attgaaattg aatacagttg ccgaaccgtt cgctataaaa gaactaactg
     1501 tggactgatt tgaaactgtc atattctgcc tcgcgtaatt tttgcggaag gtttagtgtt
     1561 cgaaagcgac ctcgtacact cccgcagatg gtctccaatc ttctcgcgat aattcggtcg
     1621 ggttcccgac cttcttcccg attcggatcg gtgtctgcgc aattgctccg gcaccgctgt
     1681 ctaggtagtc gtctggctgg tcgcgcagat ctgggttgaa gtccttcaac tgatcccaca
     1741 ccgggccttc gagcacggag acatgcgccc ataggaagcg tgaggacatg ggcgcctcga
     1801 aggcgtcgag gatgcgcttc tgcttgttgg tgacggagaa ttcttcgccc acaccgcagc
     1861 cggttccctt gagcgcctgg cgcaggatgt tcggcacgaa gccgcccggc ccgttggtct
     1921 cga
//
LOCUS       DAOTON010000587         1921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_46506_length_1921_cov_2.088960, whole genome shotgun sequence.
ACCESSION   DAOTON010000587 DAOTON010000000
VERSION     DAOTON010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46506_length_1921_cov_2.088960"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="P5292_10730"
     CDS             complement(<1..401)
                     /locus_tag="P5292_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39645.1"
                     /translation="MNCRTFSHLLLGLAMLLVTGTSMAQNAEFKWGLGFHGALVESKT
                     SLGDNFFSFKVNDKTFGQGISINRYLNKSFDLGLFLLHGAMSQKKDPYFMDDLVYTAD
                     LRLRYKLYNGYIFNEDACIGPEMFADVFEVEP"
     gene            complement(525..1304)
                     /locus_tag="P5292_10735"
     CDS             complement(525..1304)
                     /locus_tag="P5292_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRS39646.1"
                     /translation="MFAICPLSVVPVRKEPSDRSEIVTQLLFGDLVEIQDRQDNWRNI
                     KVLDDDYPGWVDKKQLRAMEESEVRTILSAPRTVTLDIVQLVVWDQHQMIPVVLGSSL
                     PGYHDKQFKISGTAFLYEGSVTQLSKPDPVHMLEHAYMYLHAPYLWGGKSPFGIDCSG
                     FTQMVYKLSGIRIRRDAWQQSEQGTPVPNIQEARAGDLAFFSNDEGKVIHTGILLPGG
                     RIMHASGRVRIDELDATGILNSDTGQLSHRLLSVRRYVNLP"
     gene            complement(1379..>1921)
                     /locus_tag="P5292_10740"
     CDS             complement(1379..>1921)
                     /locus_tag="P5292_10740"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453346.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRS39647.1"
                     /translation="VVSEDEEYKNLKADKVPSLKPAFQKDGTVTAANASKLNDGAAAL
                     VLMSKEKADQLGIRPIAKIRSYADAQQAPEWFTTAPAKALPRAIEKAGLKPEQIEYYE
                     INEAFSVVAIANNQLMKLDPARVNVFGGAVSLGHPLGASGARIVVTLISVLRQRGAKI
                     GAAGICNGGGGASAMVIELV"
BASE COUNT          448 a          491 c          498 g          484 t
ORIGIN      
        1 ggttcgactt cgaacacatc ggcgaacatc tctgggccga tacaggcatc ttcgttgaag
       61 atgtacccgt tgtacagttt gtaacgaagg cgaagatctg ccgtgtaaac caggtcatcc
      121 atgaaatagg gatccttctt ttgcgacatg gctccatgca gcaggaacaa tcccagatca
      181 aaagacttgt tcaggtaacg gttgatggag atgccctggc caaaggtttt gtcattcacc
      241 ttgaaactga agaaattatc accaaggctg gtcttcgatt cgaccaaggc tccgtggaat
      301 cccagtcccc atttgaattc agcattttgg gccatgcttg tgccggtcac cagcaacatg
      361 gcaaggccaa gcaacaagtg actaaatgtt ctgcaattca tgagttcgcg gttttggtta
      421 aggtaatgac caaattaatt gttaatttca ttaacaaaca agggcgtttt ttcaacaaat
      481 tcctaaaatg atcgccatca ggcacctaag tgggtgccga tacctcaggg aagattcacg
      541 tacctgcgca cggaaagcag gcggtgactg agttgaccgg tatctgaatt caaaataccg
      601 gtagcatcga gttcatcgat cctgactctg ccactggcat gcatgatccg gccaccggga
      661 agtaagattc cggtgtgaat gactttgccc tcatcattgg aaaaaaaagc gaggtcgcct
      721 gcacgcgctt cctggatatt tggaaccggt gttccttgct cggattgttg ccatgcatcc
      781 cgccggatac ggatgccgga aagtttataa accatctggg taaagcccga gcagtcgatt
      841 ccaaagggag attttccacc ccacagataa ggggcgtgca ggtacatata cgcatgttcc
      901 agcatgtgta ccgggtcagg cttcgatagc tgtgttacgg aaccctcata taggaaggca
      961 gttccggata tcttgaattg cttgtcatga tagccgggca ggctgcttcc gagtacgacc
     1021 ggaatcatct ggtgctggtc ccagacaact aattgaacga tgtccagtgt tacggtacgg
     1081 ggtgcgctca ggatggtgcg cacttccgat tcttccatgg cgcgcaattg tttcttgtca
     1141 acccatcccg gataatcgtc atccagcact ttgatgttgc gccaattgtc ctgtcggtct
     1201 tgtatctcca ccagatcacc gaagagcaac tgggtaacga tttcgcttcg gtcgctcggt
     1261 tcttttctga ccggtactac gcttaaaggg caaatggcaa acatgcgact aaattaatgc
     1321 aaaacgggaa aggttccagt gagcctttcc cgttttacgc aagcggatgt caacaggttc
     1381 aaaccaattc gatcaccatg gcgctggctc cgccaccacc attgcagata ccggctgcgc
     1441 cgatcttcgc accgcgctgc ctgagcacgg agatcagggt cacgacaatg cgcgcgccac
     1501 tggctcccaa tggatgtccg agtgaaacgg cgccaccgaa cacgttcacc ctggcgggat
     1561 cgagtttcat cagttgatta ttggcgatcg cgaccaccga gaacgcttcg ttgatctcgt
     1621 agtattcgat ctgttcgggt ttgagtccgg ccttttcgat ggcgcggggt agtgccttgg
     1681 caggagccgt cgtgaaccat tccggtgcct gctgggcatc cgcataggag cggatcttcg
     1741 cgatcggtcg gatgccgagt tgatcggcct tttctttgct catgagcacc aatgcggcgg
     1801 caccgtcgtt cagtttcgaa gcattggctg ctgttacagt tccgtctttc tgaaaggcag
     1861 gtttcaagga aggcacttta tcggccttga gattcttgta ctcctcgtct tctgaaacga
     1921 c
//
LOCUS       DAOTON010000588         1919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_46561_length_1919_cov_3.245172, whole genome shotgun sequence.
ACCESSION   DAOTON010000588 DAOTON010000000
VERSION     DAOTON010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46561_length_1919_cov_3.245172"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1746)
                     /locus_tag="P5292_10745"
     CDS             complement(169..1746)
                     /locus_tag="P5292_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39648.1"
                     /translation="MTFNKFLFTFLLGLAVAPSFAQTTTWSEHVAPILYAHCTSCHNA
                     AGIAPFSLMSYADAYDNANDIADAVADRTMPPWPPDPTYSGFAHQRVLSQSDRDRIAD
                     WLTDGAPEGDTALAPDPPVYSGLAELQNPDLVSRMTPYTVSTTTDLYRCFVIPTGLSA
                     DQYITQIEALPGNRGIVHHVLIFEDTSGVPAQLDAADPGPGYTNFGGTGSSSSNLIGV
                     WVPGQGMMSQPNGMGILLPANTNIILQVHYPGGTIGQTDSTEVRFRLNPGPLRRVYIA
                     SPLSHFNLDNGPLYIPADSVRTFYSHYQLPIGVSALSVGPHMHLIGRSIRSFGVTPSN
                     DTIPFIDIPEWDFHWQGLYTFPRVLHLSAGTTLYSEAFYDNTTNNPENPSDPPAPVFL
                     GEATTDEMMLVYFSYTYYLPGDENIIIDSNVVATGLPPLASPIAHTPQLYSPYPNPAS
                     DHLDFDYYLPKSSGAVLLLTDPIGRIVWKAQVDAGSTSGLQHATIQIDGFESGRYQLS
                     LQADGVLRTKAVTIIRP"
BASE COUNT          454 a          455 c          610 g          400 t
ORIGIN      
        1 aagggagaag gccaggataa gcaacaggaa tatgcggggt tttcgcatga ttagattaac
       61 aggtaataac ccgtaaagtt ccttgctgga gggctagccg gaacatcggg agtcaacggc
      121 tttcacacaa gcgcgtgtca gcagtacgat tagcatcaag aaggcgcatc aaggccggat
      181 aatggtaacc gccttcgtgc gcaggacacc atcggcctgt aaactcaact ggtagcgacc
      241 cgattcgaat ccgtcgatct ggatggtggc gtgctgcaaa ccggaagtac ttccagcgtc
      301 cacttgagcc ttccagacta ttcgtccgat aggatccgtg agtagcagga cagcgccgga
      361 ggatttgggc agatagtaat cgaagtcgag gtgatcgctt gcagggttag gataagggga
      421 atacaactgc ggtgtatggg caatcggact tgccagtggt ggaagtccgg tcgcgaccac
      481 attgctgtcg atgataatat tctcgtcgcc cggcaggtaa taggtgtagc tgaaatagac
      541 gagcatcatc tcgtcggtgg tcgcttcacc gaggaagacc ggtgccggtg gatcgctcgg
      601 gttttccgga ttgttggtgg tattgtcgta aaaagcttcc gagtacaggg tggtgccggc
      661 cgacaggtgg agaacgcgag gaaaggtgta gagtccctgc caatggaaat cccattcggg
      721 gatgtcgatg aacgggatcg tatcgttgga aggcgtcacc ccgaacgatc gtatgctgcg
      781 tccgatcagg tgcatgtgtg ggccaacgga gagggccgaa acaccgattg gtaactggta
      841 atgagaataa aacgtgcgga cagaatcagc gggaatgtag agtggaccat tgtcgaggtt
      901 gaagtgcgac aggggcgatg cgatgtagac ccgtcgcagc ggaccggggt tcagccggaa
      961 gcgcacttcg gtggaatcgg tctgcccaat ggtaccaccg ggatagtgga cctggagaat
     1021 gatgttggtg ttcgcgggca gcaggatgcc catgccgttg ggttgggaca tcataccctg
     1081 tccgggtacc cacacaccga tcaggttgga actcgaagat ccggttcctc caaagttggt
     1141 gtagccggga ccgggatcgg cggcgtcgag ctgggccgga acgccggagg tgtcttcgaa
     1201 gatcaggaca tggtgtacga taccccggtt gcccggcagc gcttcgatct gcgtgatgta
     1261 ctgatcggcc gataatccgg tcgggataac gaagcaacga tagagatccg tcgtagtgct
     1321 taccgtataa ggagtcatgc gactcaccag atcgggattc tggagttcgg cgagtccgga
     1381 atagaccggc ggatcgggag ccagcgccgt atccccttcg ggtgcgccat cggtcagcca
     1441 atcggcaatg cgatcgcgat ccgactgact caacacccgt tgatgcgcaa atccgctata
     1501 agtgggatcg ggcggccagg gcggcatcgt gcggtcggct accgcatcgg cgatatcatt
     1561 cgcattgtca taggcatccg catagctcat caacgaaaac ggcgcgatgc cggcagcatt
     1621 gtgacaggat gtacagtgcg cgtagaggat cggcgccacg tgctcggacc aggtggtcgt
     1681 ttgcgcgaac gagggtgcaa ctgccagacc tagcagaaag gtgaatagaa atttgttgaa
     1741 agtcatagca gacgcaactt gtcccatgaa ggtagcgatt ttattgattc gggtgtccgg
     1801 ggtagtaaaa gccgtatacg aatagcctct caacattcaa gttgattatt acgacaatag
     1861 cttccatcac tcctctggca acgttgaaag ggcctgattt acccttgaga gtcgtgtga
//
LOCUS       DAOTON010000589         1918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_46588_length_1918_cov_3.556092, whole genome shotgun sequence.
ACCESSION   DAOTON010000589 DAOTON010000000
VERSION     DAOTON010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46588_length_1918_cov_3.556092"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="P5292_10750"
     CDS             complement(<1..415)
                     /locus_tag="P5292_10750"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRS39649.1"
                     /translation="MKIFKRVLIAIAILLLVIAAIGFMFPSKVHVERATVINQPKEKV
                     FAYVNNLHNFNSWSPWHDMDTAATYTYEGPETGVGAAMKWESNNKDVGKGMMKFVEVV
                     DGQMIREELSFMDEGSAFATFTFAPEEAGTRVTWAF"
     gene            complement(412..699)
                     /locus_tag="P5292_10755"
     CDS             complement(412..699)
                     /locus_tag="P5292_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRS39650.1"
                     /translation="MRKSKFTEVQIVAILAQYEQGVKVEDICRENGISQPTFYQCQRK
                     YSGMEVSELKRLKEMEQELAKFKRRICIARCLVFHKKITTLSPSTLFATQS"
     gene            complement(851..>1918)
                     /locus_tag="P5292_10760"
     CDS             complement(851..>1918)
                     /locus_tag="P5292_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRS39651.1"
                     /translation="NRNAINKLVASDIGATLKLKDTLTNHTLCAAGKDFQFDPIHFPA
                     PRLQTAIVAKNKADDEKLGTVLQEIHQEDPTLEVDYNRELKQVLLGSQGELHLAVTKW
                     RLEKVYHLAVEFEKPRIPYRETIVKASMASYRHKKQSGGAGQFGEVYLKIEPYHDGMP
                     EPHEHHVRDKEVLDLPWGGKLVFYNCIVGGVIDQRFIPSIQKGIMEKMHEGPVTGSYV
                     RDIRVIVYDGKMHPVDSNDISFKIAGMMAFKEAFHQAEPRILEPVYEIEVMVPEELMG
                     DVVTDLQGRRSIILGMDSKGKYQVIKARAPLAELDKYSTSLRSITQGRGSYSGKFLEY
                     APVPGEIQRKLAEGYKATEQH"
BASE COUNT          354 a          514 c          556 g          494 t
ORIGIN      
        1 caaacgccca ggtcaccctg gtgcccgctt cttccggagc aaaggtaaag gtcgcgaagg
       61 ccgagccttc gtccatgaag ctgagctctt ccctgatcat ctgcccatcg accacctcga
      121 cgaacttcat catgcccttc ccgacatcct tgttgttgga ctcccacttc atcgctgcgc
      181 cgacgccggt ttccggccct tcgtaggtgt aggtcgcggc cgtatccata tcatgccagg
      241 gcgaccagct gttgaaattg tggaggttgt tcacataagc aaagactttt tccttcggtt
      301 ggttgatgac cgtagcccgt tccacatgga ccttcgacgg gaacataaaa ccaatagcgg
      361 caatgaccaa caagaggatc gcgatggcga tcaggacgcg tttgaagatt ttcatgactg
      421 tgtggcaaac aaggttgagg gggataatgt cgtgattttt ttatgaaata caagacatcg
      481 cgcgatacag atccttcgtt tgaacttggc cagttcctgt tccatctcct tgagccgctt
      541 gagttcggaa acttccatcc ccgagtactt ccgctgacat tgatagaatg tcggctggct
      601 gataccgttc tcccggcaaa tatcctccac ctttacgcct tgttcatact gcgccaggat
      661 ggccacaatc tgaacctctg tgaactttga ttttcgcata cgctttacga atttagtagt
      721 ttagagcgta gctgtttttg gggaagcctt caggtggaac cgttggtgat gggccacaga
      781 tatgatattc attgaataac aagggccggt accattaccg gcccttgtta ttaagtacag
      841 gaataatagc tcaatgctgc tcggtcgcct tgtatccttc ggcgagtttg cgttgaattt
      901 cgccggggac gggcgcgtac tcgaggaatt ttccggagta gctgccgcgg ccttgcgtga
      961 tggagcgtaa ggaggtggag tacttgtcga gttccgccag gggcgcgcgg gctttgatga
     1021 cctggtactt gcctttgctg tccatgccta ggatgatcga gcgccggcct tgcaggtcgg
     1081 tgaccacgtc gcccatcaat tcttcgggca ccatgacttc gatctcgtac accggctcga
     1141 ggatgcgcgg ctcggcctgg tggaaggctt ccttgaaggc catcatgccg gcgatcttga
     1201 aggagatgtc gttggagtcg acgggatgca tcttgccgtc gtacacgatc acgcggatgt
     1261 cgcgcacgta ggagccggtc accggacctt cgtgcatctt ttccatgatg cctttctgga
     1321 tcgacgggat gaaccgctgg tcgatcacgc cgccgacgat gcaattgtag aagaccagct
     1381 tgccgcccca gggcaggtcg agcacttcct tgtcgcgtac gtggtgctcg tgcggttccg
     1441 gcatgccgtc gtggtagggt tcgatcttga ggtacacttc tccgaactga ccggcgccgc
     1501 cgctttgctt cttgtgccgg tacgaggcca tcgaggcttt cacgatggtc tcgcggtagg
     1561 ggatgcgcgg cttttcgaat tccacggcga ggtgatagac cttttccagc cgccatttcg
     1621 tgacggccag gtgcagttca ccctgtgaac cgagcagtac ctgcttgagt tcgcggttgt
     1681 agtcgacttc cagggtcggg tcttcctgat ggatctcctg caggacggtg ccgagttttt
     1741 cgtcgtcggc cttgttcttc gccacgattg cggtttgcag gcgcggagcc gggaagtgga
     1801 tcgggtcgaa ttggaaatcc ttgcccgctg cgcagagcgt gtggttggtc agcgtgtctt
     1861 tcaacttgag cgtcgcgccg atgtcgctgg caaccagctt gttgatggcg ttgcggtt
//
LOCUS       DAOTON010000590         1917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_46628_length_1917_cov_2.897959, whole genome shotgun sequence.
ACCESSION   DAOTON010000590 DAOTON010000000
VERSION     DAOTON010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46628_length_1917_cov_2.897959"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            486..>1917
                     /locus_tag="P5292_10765"
     CDS             486..>1917
                     /locus_tag="P5292_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS39652.1"
                     /translation="MGDLINQTVAAANEKKNGQDYSAESIQILEGLEAVRKRPAMYIG
                     DIGVKGLHHLVYEVVDNSIDEALAGFCKNIEVFIHPDNSISVIDDGRGIPTDIHPKEK
                     RSALEIVLTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSTHLKVVVHREGKIFEQEY
                     ERGKPLYPVREAGTADRTGTTVTFLPDNTIFNSTVYHYDTLCARLRELAFLNKGIRLN
                     ITDERETDADGNHPTESFYSEGGLREFVSYLDENRERLIAQPIYMEGERAGIPVEVAM
                     LYNSSFNENIHSYVNNINTHEGGTHLSGFRRALTRTLKSYADKSGLLEKVKFEISGDD
                     FREGLTAIISVKVAEPQFEGQTKTKLGNSEVMGAVDQAVSDMLESYLEEHPREAKMIV
                     DKVILAATARHAARKARELVQRKNALSSTGLPGKLADCSETDASACELYLVEGDSAGG
                     SAKQGRDRRFQAILPLRGKILNVEKAM"
BASE COUNT          486 a          516 c          495 g          420 t
ORIGIN      
        1 atacacctac tgagaagtat cagaagctgg ccagtaagaa tccgctcctg cacgaattac
       61 gtaagcgatt ggatctggaa acggagtatt aaagccggtt ttttcgatcc ggttttggga
      121 tcacgatcaa ttgtttttcg cattcctatc gggttattaa caagccttgc taaaagttcg
      181 aaaaacgatt aaaaacaagg tttttgaaac tcgccaaaag tcagtttttg cttaaatttt
      241 caacttttct ggcacccatc cgggtcatcg cttgggatgg gtaattattt gcatgaattc
      301 caattattta aagcattaaa agcggctttc atacgagtcc gaaaatgttg attttaagat
      361 aaaatccgct ttgtcatgct gcatcatttt cgtatcttca ccaaccaggt caaaattccg
      421 ctggattctt ttgtcaaaaa cagcgatgga cctgtatcat cgggggggac tattttccat
      481 caatcatggg cgacctgatc aaccaaactg tggctgcagc taacgaaaag aagaacggac
      541 aagattattc ggccgagagt atccagattc tggaaggtct cgaagcggtg cgtaaacgcc
      601 ccgcgatgta catcggcgat atcggcgtaa aaggattgca tcacctggtg tacgaagtag
      661 tcgacaattc gatcgacgaa gcgttggcgg gtttttgcaa gaacatcgag gtgttcattc
      721 accccgataa ttccatctcc gtcatcgacg acggacgtgg tatcccgacc gacatccacc
      781 ccaaggagaa acgcagcgcg ctggaaatcg tgctcaccgt cctgcacgcc ggtggtaagt
      841 tcgacaagga cagctacaaa gtatccggtg gtctgcacgg tgtcggtgtt tcctgtgtga
      901 acgcgttgtc gacgcatttg aaagtcgtgg tacatcgcga aggcaagatc ttcgagcagg
      961 aatatgaacg cggcaaaccg ctctatccgg ttcgtgaagc cggaacggcg gaccgcacgg
     1021 gaacgacggt cacttttctt cctgataata ccatcttcaa cagcacggtt tatcattacg
     1081 ataccttgtg cgcacggttg cgggaactcg ccttcctcaa taaaggcatc cggctcaaca
     1141 tcacggacga acgtgaaacc gacgcggacg gcaatcatcc gacggaatcc ttctactccg
     1201 agggcggact acgggaattc gtttcctacc tcgacgagaa ccgggagcgg ctcatcgccc
     1261 aaccgatcta catggaaggc gaacgcgccg gcattccggt cgaagtggct atgctataca
     1321 acagctcgtt caacgagaac atccacagct acgtcaacaa catcaacacg cacgaaggcg
     1381 gcacgcacct ttccggcttc cgtcgcgcgc tgacccgcac cctcaagagc tatgcggata
     1441 aatccggtct tctggaaaaa gtcaagttcg agatctcggg cgacgatttc cgggagggtc
     1501 tgacagcgat catttcggtg aaggtcgccg agcctcagtt cgaaggacag accaagacca
     1561 aactcggcaa cagcgaggtc atgggcgcgg tcgatcaggc cgtctccgat atgctggaat
     1621 cctacctcga agaacatccg cgcgaagcga agatgatcgt cgacaaggtg atcctggccg
     1681 ctactgcccg acatgccgcc cgcaaggcgc gtgaacttgt gcaacgcaag aacgcgctct
     1741 cgagtacggg cctccccggc aaactggccg actgttccga aaccgacgct tccgcttgcg
     1801 aattgtacct cgtcgagggc gactccgccg gcggctcggc caaacagggc cgcgaccgtc
     1861 gcttccaggc catcctgccg ctgcgcggta agatcctgaa cgtcgagaaa gcgatgg
//
LOCUS       DAOTON010000591         1917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_46629_length_1917_cov_2.886144, whole genome shotgun sequence.
ACCESSION   DAOTON010000591 DAOTON010000000
VERSION     DAOTON010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46629_length_1917_cov_2.886144"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1917
                     /locus_tag="P5292_10770"
     CDS             <1..>1917
                     /locus_tag="P5292_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39653.1"
                     /translation="DFVVTQITLAQASQPTTATSNLALATGNCNDLTLRWTNGNGARR
                     LVVARQNGSVNALPIDGSYYTANSVFGLGSNLGSGNYVVYDRTDTTVTVTGLTVGERY
                     FFAVIEYNGTGFTTNYLTTGYPQANQVANSFNFTVSTNTSQLCNGGSATLTASTASSY
                     SWSPATGLSSTTSQIVTATPTVTTTYTVTGNDGSGCIAVAHVTITVGTQPTVILNTLN
                     AVCTNSAAFALTTGTPTGGTYSGPGVSAGNFNPSAAGAGVHIITYTVNSGGCSGTDTA
                     TIRVNAAPVVSFATLAPACSNGSAVTLSGGSPAGGTYSGTGVSGSSFNPVTAGTGTFV
                     LTYAYTDNNGCSGNDTSAITVNAAPTVSLGSFTAVCQNAGVQTLSGGSPAGGTYSGPG
                     VSGTSFDPAVAGAGNHTITYAYTDAAGCSNSATATWRVNALPNVTLATLPNVCSNTPV
                     MNLTGGSPAGGTYSGTGVSGSTFNPAVSGNGTFAITYTYTDNNGCTNSAQRNIVVNAA
                     PSVTFSTINPVCLNASPVTLTGGSPAGGTYVGTGVSGGQFNPSVGIGTYSIQYLYTDM
                     NGCTGSASQNVQVRPLPTVALATLPAQCSNSAPLTLTGGSPAGGTYSGTGVSGGAFDP
                     SVSGTGTFNITYTYT"
BASE COUNT          394 a          611 c          510 g          402 t
ORIGIN      
        1 cgatttcgtg gtgacccaga tcaccctggc gcaggccagt cagccgacta cggcaaccag
       61 caatcttgcg cttgctaccg gtaactgcaa tgaccttacc ctgcgctgga ccaacggcaa
      121 tggagcccga cgacttgttg ttgcccgaca gaatggctcg gtgaacgcgc tgccgattga
      181 tggaagttat tacacggcca atagcgtatt cggattgggg agcaatttgg gctcgggaaa
      241 ttatgtcgtc tatgaccgaa ccgatacaac agtcactgta accggcctga cggtcggaga
      301 acgctacttc ttcgccgtca tcgaatacaa cggcaccggc tttacaacga attacctcac
      361 gaccggctac ccgcaagcca accaggtcgc caacagtttc aacttcacgg tttcgaccaa
      421 tacgtcgcaa ctttgcaacg gaggatcggc taccctcacc gccagcaccg cgtccagtta
      481 ttcctggtcg ccagcaacgg gtttgtcctc taccacttcg cagatcgtca ccgccacccc
      541 tacggttacc acgacttata ccgtcaccgg taacgatggc agcggttgta tcgcggttgc
      601 ccatgtcacc atcaccgtgg gtacgcaacc gacggtcatc ctgaatacgc tgaatgcggt
      661 ctgtaccaac agtgccgctt tcgcgctgac gaccggaaca ccaacaggtg gcacctacag
      721 cggccctggt gtcagcgccg ggaatttcaa tccttccgct gccggtgccg gtgtgcacat
      781 catcacctat acggtgaaca gcggcggttg ctccggtacc gataccgcga ccatccgggt
      841 gaatgccgct ccggtggtta gctttgccac gcttgctccg gcttgttcca atggaagtgc
      901 tgtcacgctg agcggtggct cgcctgccgg cggcacgtat agcggaaccg gcgtcagcgg
      961 ttcaagcttc aatccggtaa cagccggtac gggaactttt gtactgacgt atgcctatac
     1021 ggataacaac ggttgctccg gaaacgatac ttccgccatc acggtgaatg ccgctccgac
     1081 ggtttcgttg ggaagcttta cagcggtatg tcagaatgcc ggggtacaga cgctgtccgg
     1141 cggttcgcct gccggcggca cgtacagcgg acctggtgtc agcggaacga gtttcgatcc
     1201 cgctgtagca ggcgccggta accatacgat cacctacgct tacaccgatg ccgccggttg
     1261 cagcaattcc gcgaccgcca cctggcgcgt caacgctttg ccgaacgtta cgctggcaac
     1321 attgcccaac gtctgcagca acacccctgt aatgaacctg accggcggat cgcctgccgg
     1381 tggtacctac agcggcacag gtgtgagtgg cagcaccttc aaccctgctg tatcgggtaa
     1441 cggtacgttt gccatcacct atacgtatac cgataacaac ggctgtacga attccgcgca
     1501 gcggaacatc gttgtgaatg ctgctccaag cgtcacgttc tccacgatca atccggtttg
     1561 cctcaacgct tcaccggtta ccctcaccgg cggatcgcct gcaggtggga cctatgtggg
     1621 taccggtgtg agcggcggac aattcaatcc gtcggtcggc atcggaacat actccatcca
     1681 atatctctat accgatatga acggttgtac cggttctgcc tctcagaacg tccaggtgcg
     1741 accgctcccg accgttgcac ttgccaccct gccggcgcag tgcagcaaca gcgctccgct
     1801 caccctcacc ggcggttctc ccgcgggcgg tacctatagc ggcaccggtg tatccggcgg
     1861 cgcattcgat ccgtccgttt ccggtaccgg tacatttaac atcacctaca catacac
//
LOCUS       DAOTON010000592         1907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_46969_length_1907_cov_2.744600, whole genome shotgun sequence.
ACCESSION   DAOTON010000592 DAOTON010000000
VERSION     DAOTON010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46969_length_1907_cov_2.744600"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="P5292_10775"
     CDS             complement(<1..639)
                     /locus_tag="P5292_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRS39654.1"
                     /translation="MFQKARETTFLESTSLLMTVLRWRKPLLAVTFISALAAGIFSGP
                     SFITPKYKSTVIFFPPATNSISKALLDEHSSDKQDILAFGEEEQAEQMLQILNSDEIR
                     EAIIRKYNLMQHYGISPDESYPLTRLSEEFHDNISFSRTEFMSVRIDVLDKDPAMAAS
                     IANDIAALLDSMKSKIQRSRASEALLIVEQTYQEKLQASAVKEDSLTRLREMG"
     gene            complement(788..>1907)
                     /locus_tag="P5292_10780"
     CDS             complement(788..>1907)
                     /locus_tag="P5292_10780"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRS39655.1"
                     /translation="KLLLAVLVNQILISMIMYLRSNLAGLHLFKTDSIISVLDRLIMI
                     VICGFLLLTHRDPGTFELRWFVYAQTAAYIITFLITFMIVIDKAKVRRFHWRWPFFVM
                     ILKKSYPYAVLVLLMTFYNRIDGVMLERMLPFREGAVEAGIYASAYRLLDAANMIAFL
                     FAGLLLPIFARMIKLRQHVEGMVRLSFSLLVVPALVVAICCWFYSQELMELLYRGEHI
                     DESAAVFRILMCCFVPISSTYIFGTLLTANGSLRQLNLMATTGMLINILLNLYLIPRM
                     EARGAAISSITTQLLTAIAQILMVQYIFRFKINYTLLVGLGAFAASCIAWCWFIRGNE
                     LNWMLQFGLAAAGCFALALVTRLISPKNIYRIIRYDES"
BASE COUNT          479 a          493 c          547 g          388 t
ORIGIN      
        1 gcccatttcc cgcaagcgag tcagcgagtc ctctttaaca gcggatgcct gcagtttctc
       61 ttggtacgtt tgttcgacga tgaggagcgc ttcggaagcc cgggaacgct ggatcttcga
      121 tttcatggaa tccaacaagg ccgcgatatc gttcgcgatc gaagcggcca tcgcggggtc
      181 cttatccaac acgtcgatgc ggaccgacat gaattctgta cgggaaaacg aaatgttgtc
      241 gtggaactcc tcgctgaggc gtgtcaaagg ataggactca tccggggaaa tgccataatg
      301 ctgcatcagg ttgtacttcc ggatgatcgc ttcccggatc tcgtccgagt tcaggatctg
      361 cagcatctgc tcagcttgct cttcttcccc gaaggccagg atgtcctgct tgtcggatga
      421 gtgttcgtcc aacaacgcct tactgatcga attcgttgcc ggagggaaaa agataacagt
      481 cgacttatac ttgggtgtaa taaaggaggg acctgaaaaa attccggcag ccagggcgga
      541 aatgaatgtc accgccaaca atggcttccg ccaccgcagc acggtcatca acaaactggt
      601 ggattccagg aaggtggttt ccctggcttt ctgaaacatt ggttagcgct aaaattttgg
      661 gaacgaaggg cttcaaaggt aggaaaggat actatacaaa gaaagccaca cagggtgcaa
      721 aagaagctga aaatgaatgt tatgcggaag cggtgtgctc tcccggcccc aaggacggca
      781 cccaggctca actctcgtcg taccggatga tccgataaat gttcttcggg ctgatgagcc
      841 gggtgacgag tgccagggcg aaacaacctg ctgccgccaa gccgaattgc aacatccagt
      901 tcaattcgtt cccccggatg aaccaacacc aggcgatgca cgaagcggcg aacgctccga
      961 ggccgacgag cagcgtatag ttgatcttga agcggaatat gtactgtacc atcaggatct
     1021 gagcaatcgc cgtgagcagt tgggtcgtga tactcgatat cgcggctccc cgggcttcca
     1081 tccttgggat caggtagaga ttcagcagga tgttgatcag catcccggtg gtagccatca
     1141 ggttcaactg gcgcaggctg ccattcgcgg taagcagggt tccgaaaatg taggtggaag
     1201 agatcggtac aaaacagcac atgaggatgc gaaagaccgc ggcggattcg tcgatgtgct
     1261 caccacggta gagcagttcc atcagttcct ggctgtaaaa ccaacaacag atagccacta
     1321 cgagggccgg cacgaccagc agcgaaaacg atagccgtac catgccttcc acatgctggc
     1381 gcagtttgat catgcgggcg aagatgggta acaacagacc cgcgaagaga aaggcgatca
     1441 tgttggcggc atcgagcaga cggtaggcgg aggcgtaaat gcccgcttcc accgcccctt
     1501 cccggaaggg caacatacgt tccagcatca ccccgtcgat gcggttatag aaggtcatga
     1561 gcagcaccag cacggcgtac ggataactct tcttcaggat catgacgaag aatggccagc
     1621 gccagtggaa tcttcgcacc ttcgccttgt cgatcacgat catgaaggtg atcaggaacg
     1681 tgatgatgta ggccgcggtt tgcgcgtaca cgaaccagcg caactcgaag gtgcccgggt
     1741 cccggtgcgt caggagtagg aagccgcaga tcacgatcat gatcaggcgg tcaagtaccg
     1801 atatgatgct gtcggttttg aagaggtgca agcccgcgag attcgatcgc aggtacatga
     1861 tcatcgagat caggatctgg ttgaccagca ctgccagcag cagcttc
//
LOCUS       DAOTON010000593         1905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47026_length_1905_cov_3.589730, whole genome shotgun sequence.
ACCESSION   DAOTON010000593 DAOTON010000000
VERSION     DAOTON010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47026_length_1905_cov_3.589730"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1905)
                     /locus_tag="P5292_10785"
     CDS             complement(<1..>1905)
                     /locus_tag="P5292_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS39656.1"
                     /translation="AQCYNEIGKIYIYLQDYPNAIEHFRKALRIYEGLKLRPEMVNSF
                     FFLGNAYNWADEFDKSLYYLLRAQNALDEIKDIDIRTKVLGSLAILHTKLKEYDKSLN
                     YFNEALRAAEDGASPTVKAQLKKSLGNLYIELTQYDKAIDVLLSAMEAAQSSPLEAQL
                     VKIHQFLATAYEKIGDHASALEHFKKYFDLDREILSEEVSLKTKALHVKYDLEELRKQ
                     KEIAELSDKLKEQFLANVSHEIRTPMNGVLGMAHLLARTNPTREQQEYIDAIRLSANN
                     LMVIINDILDFSKINAGKIEFSETEFNIRELMKGIVQILQVKAEEKKIQIGCTIDYHV
                     KDVLSGDPIRLNQILVNLMGNAVKFTEKGKVMLDIRVLESREQSCRLRFRVTDTGIGI
                     PDNKLHKIFESFEQVEDHKRRFEGTGLGLTIVKQLVELQGGSISVRSKVGEGSEFTVD
                     MNFRIGKQVGQQPVAEPIPSEQQDVQHVRVLVVEDNKVNQLLVKNMLRKFGFEQFDTA
                     SNGREALEQLRNQDYDIILMDIQMPEMDGYEITREIRQRMRREIRQVPIIALTADASD
                     KEKARAKEAGMNDYVVKPYTPEELYNAMARFIPDPGTPVPARDAVPSRSAQPGMSIDF
                     LEKYTGGDQEL"
BASE COUNT          361 a          508 c          519 g          517 t
ORIGIN      
        1 agttcctggt cgccgcccgt atacttttcc agaaagtcga tgctcatgcc cggctgtgcg
       61 gaacgggacg ggaccgcgtc acgagcagga acaggcgtac ccggatcggg aatgaaacgg
      121 gccattgcat tgtacaattc ttccggagtg tagggcttga cgacgtaatc gttcatgccc
      181 gcttccttcg cccgcgcctt ttccttatcg gaagcatcgg cggtgagcgc gatgatcggg
      241 acctgacgga tctcccgccg catccgctgc cggatctcgc gtgtgatctc atagccgtcc
      301 atctccggca tctggatatc catcaggatg atatcgtaat cctggttgcg gagttgctcc
      361 agggcttccc gcccgttgga tgcggtgtcg aactgttcga aaccgaattt ccggagcatg
      421 ttctttacca gcagttggtt gaccttattg tcttccacaa ccaatacacg gacatgctgt
      481 acatcctgtt gttcggatgg gatcggttcc gctacgggtt gttgaccgac ctgttttccg
      541 atccggaagt tcatatcgac cgtaaactcg gatccttcgc cgaccttact gcggactgaa
      601 atggaaccgc cctgcagttc gaccaattgt tttacgatgg tgagaccaag tccggtgcct
      661 tcgaaacgtc gcttatgatc ttccacctgc tcgaagctct cgaagatctt gtgcagcttg
      721 ttatccggga tgccaatacc cgtgtcggtc acgcggaacc gcagccggca gctttgttca
      781 cgactttcca gtacccggat gtccagcatc actttgccct tctcggtgaa cttgacggca
      841 ttgcccatca ggttgaccag gatctggttc aggcggatcg ggtctccgga taggacatcc
      901 tttacgtggt agtcgatggt gcagccgatc tggatcttct tctcttccgc ctttacctgc
      961 aggatctgca cgatgccttt catcagttcg cggatgttga attccgtctc gctgaattcg
     1021 atcttccccg cgttgatctt ggagaagtcg aggatgtcgt tgatgatcac catcaggttg
     1081 ttggccgaca aacggatggc atcgatgtat tcctgctgtt cccgggtagg gttggtgcgc
     1141 gccagcaggt gggccatgcc caacacgccg ttcatcgggg tgcggatctc gtggctgacg
     1201 ttggctaaga attgttcctt taatttatcg gaaagctccg ctatctcctt ttgcttgcgc
     1261 aactcttcca ggtcgtactt cacatgcagg gctttcgttt tcaagctgac ttcctcgctg
     1321 aggatttccc ggtcgaggtc gaagtacttt ttgaaatgtt ccagcgcgga ggcatggtcg
     1381 ccgatcttct cgtaagcggt tgccagaaac tgatggatct tgactagctg cgcttccagg
     1441 ggtgacgact gtgcagcttc catggcggaa agcaggacat cgattgcctt gtcgtactgc
     1501 gtaagttcga tatacagatt gccgaggctc ttcttcaatt gcgccttgac cgtagggctc
     1561 gcgccgtctt cagcggcacg aagggcttcg ttgaagtaat tcagcgattt gtcgtattcc
     1621 ttcagcttgg tgtgcaggat ggccaggctt ccgagtactt tggttcggat gtcgatatcc
     1681 ttgatctcgt ccagcgcgtt ttgcgcacgt aggaggtagt aaagcgattt atcaaactca
     1741 tcggcccagt tataggcgtt gccgaggaag aagaatgaat tgaccatttc cggccgcaac
     1801 ttcaatcctt cgtagatccg aagcgccttg cggaaatgtt cgatggcgtt gggataatcc
     1861 tgcaggtaga tgtagatctt tccgatctca ttgtagcact gcgca
//
LOCUS       DAOTON010000594         1901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47148_length_1901_cov_3.027086, whole genome shotgun sequence.
ACCESSION   DAOTON010000594 DAOTON010000000
VERSION     DAOTON010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47148_length_1901_cov_3.027086"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1901)
                     /locus_tag="P5292_10790"
     CDS             complement(<1..>1901)
                     /locus_tag="P5292_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRS39657.1"
                     /translation="NRLNAFNLAFNDVTRVLNAENVETPSGKVYGNQTELTIRAMGRL
                     RTEEEFRLLVLREDADGIVRLGDVARVELGPEQYEQSWKLNGLPGVGLAIIPQPGANY
                     IKIADEYYKRLAEIQKGNATDIQLSTLIDSTRNVRRSLLEVEETLLIAFSLVVLVIFF
                     FFRNWKIALRPLIDIPISLIATFFIMYLAGFSINILTLLGIVLATGLVVDDGIVVTEN
                     IFRKLEQGMPIRQAAREGSKEIFFAVVSTSATLAIVFLPVIFLEGFVGSLFREFGIVV
                     AGAVLISAFVSLTITPVLNVLLQSKDKGHGRFYERTEPFFRAMESGYRRRLDRFLHRR
                     WLAVGIVLACLALIFFIGRSLQSELAPLEDKSSIRFSISGPEGASYGFMSQAGEEFGN
                     FLIDSLKEKEFAFLAVPSFFGTSINSGFGRAALVPPDQRERSQSEIARDLAKKATRFN
                     NLRIFPIEEQTISVGLGSRGSLPVQFVLQNLDFDKIKSVLPRFLEAARANKTFQNVDV
                     NLKFNRPEMNIEIDRIKARELGLTVSDISTVIQSAFSGRRIAYFTMNGKQYSVYSQVD
                     LSDRQKPADITDLYVRTPAGEMVSMASLVMMKPSASPPTLYHFNRFKSATVSASLAEG
                     KTIGDGVEV"
BASE COUNT          427 a          537 c          541 g          396 t
ORIGIN      
        1 cacttcaacg ccgtctccga tcgtcttccc ttccgcgagg gatgccgaaa cggtagccga
       61 cttgaatcga ttgaaatgat acagggtagg cgggctggcg gaaggtttca tcatcaccaa
      121 agatgccatg gaaaccattt cgccggcggg cgtgcgaaca tacaagtcgg tgatgtcggc
      181 aggcttctgt cgatcagaaa gatccacctg ggaataaacc gaatactgct tcccgttcat
      241 ggtgaaatac gcgatgcgcc ggccggaaaa tgccgactgg atcaccgtgg agatatcgct
      301 gaccgtcagt cccaactcgc gcgccttgat ccggtcgatc tcgatgttca tctccggtcg
      361 gttgaacttg aggtttacgt ctacgttctg gaaggtcttg ttcgcgcggg ctgcttcgag
      421 gaaacgaggc aacacgcttt tgatcttgtc gaagtcgagg ttctgcaaaa cgaattgtac
      481 cggtaacgaa ccgcgggacc caaggccgac ggagatggtt tgttcttcga tcgggaagat
      541 gcgcaggttg ttgaaccgcg tggccttttt ggccaggtcg cgtgcgatct ccgattgcga
      601 acgctcgcgt tgatccggtg gcaccaatgc ggcacggccg aaacctgaat tgatgctggt
      661 gccgaagaag ctgggtactg cgagaaaagc gaactccttt tccttcaacg aatcaatcag
      721 aaagttgccg aactcctcgc ccgcctgcga catgaaacca tagctcgctc cttcgggacc
      781 gctgatggaa aaacggatgc tgcttttgtc ctccagcggc gccagctcgc tttgcaggct
      841 gcggccgatg aagaatatca acgcaaggca cgccagcacg atgccaacgg ccagccagcg
      901 acggtgcagg aaacggtcga ggcggcggcg atagccggac tccatagctc taaagaatgg
      961 ttcggtccgc tcgtaaaacc taccgtgacc tttgtcctta ctctgcaaga gtacattgag
     1021 taccggagta atggtcagtg aaacgaacgc cgagatcaag acggccccgg ccacgacgat
     1081 gccgaactcg cggaagagac tgccgacaaa gccctcgagg aagatcaccg gcagaaacac
     1141 gatcgccagc gtagcggacg tagagaccac cgcgaagaag atctccttcg atccctcccg
     1201 ggcggcctgc cgaatgggca tcccctgctc cagctttcgg aagatgttct ccgtgaccac
     1261 gatcccgtca tctacaacga gtccggtggc cagtacgatt cccagcaagg tgaggatgtt
     1321 gatcgagaag cctgccaggt acatgatgaa gaaggtcgcg atgagtgaga tcggaatatc
     1381 gatcagcggt cgcagggcga tcttccagtt acggaagaag aaaaagatga cgagcacgac
     1441 cagggagaac gcgatcaaca gcgtctcttc cacttcgagc agggagcgtc gaacgtttcg
     1501 cgtactgtcg atcagtgtac tcaattgaat atcggtggca ttgcctttct ggatctccgc
     1561 cagtcgcttg taatattcgt ccgcgatctt gatgtagttg gcgccgggct gcgggatgat
     1621 ggcaagcccc acgccgggca agccattcag cttccaactc tgttcgtatt gctccgggcc
     1681 cagctcgacc cttgccacat ctcccaggcg gacgatgccg tcggcgtctt cgcgcaatac
     1741 cagtagccga aactcctctt cggtacgcag tcgtcccatc gctcgaatag tcagttccgt
     1801 ctggttgccg tacaccttac cggagggcgt ttcgacattc tccgcgttca atacccgcgt
     1861 aacgtcgttg aacgccaggt tgaacgcgtt caggcggttc g
//
LOCUS       DAOTON010000595         1899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47206_length_1899_cov_2.605206, whole genome shotgun sequence.
ACCESSION   DAOTON010000595 DAOTON010000000
VERSION     DAOTON010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47206_length_1899_cov_2.605206"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="P5292_10795"
     CDS             <1..246
                     /locus_tag="P5292_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39658.1"
                     /translation="QDLHAIECKHLVEAGKSTRYPDAYAFRSAAFQEILRDLDRTVLA
                     EYESYYQQLADLEFRMSESEKQAYYKATEDLYKGECR"
     gene            complement(259..1806)
                     /locus_tag="P5292_10800"
     CDS             complement(259..1806)
                     /locus_tag="P5292_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HRS39659.1"
                     /translation="MAETGFRKWIVTLTVILASLLELIDTTVVNVSLPQIMGNLGATL
                     EDVGWVVTAYAVANVIILPISGWLGARFGRKRYFTFSIILFTVSSFFCGNADNIWELV
                     IFRFLQGIGGGALLGTSQAILMEAWPPAQMGMATALFGLGVVVGPTLGPTLGGYITDH
                     FSWPWVFYVNIPLGIIAVLLALTYIRESKYERKIGAVDWWGIIFLTIGVGALQTVLER
                     GESEDWFETPYITLLSIIALSGIVAFIRRELTTEHPIVDLRILKNRSLAIGMFTTFIL
                     GFGLFGSVFVFPVFCQNLLGFSAQQTGMLLIPGGLATIFMMPLVGKLLQRGVPPQIMG
                     TIGFLFFFLFTYFLSRSTLNSGESDFYLPLILRGIGLSLLFVPLTTLALSSLKPSDIA
                     QGTGLNNMMRQLGGSFGIAIITTLIHLRQGFHRNHLIEHVSTSDPATMERMKVVVANF
                     IAHGYAPGQAEQMALKALEGAVQKQTFLLSYMDAFWLTGIFFIFSIPLLFLQKFNRRA
                     KLDPSAH"
BASE COUNT          520 a          497 c          496 g          386 t
ORIGIN      
        1 caagatcttc atgcgatcga atgcaaacac cttgttgaag caggcaagag cacccgttac
       61 ccggacgctt atgcattccg ttcggcagcg tttcaggaaa tcctgcgtga ccttgaccgt
      121 accgtattgg cggaatatga atcgtactat cagcagcttg ccgatctgga attccgaatg
      181 agcgaatccg aaaagcaggc ttattacaaa gcgacagagg acctctataa aggagaatgc
      241 cggtaaaatc gaatctcgtt aatgagccga agggtccagt ttggcgcggc gattgaactt
      301 ctgcaggaat aacagcggaa tggagaaaat gaaaaatatg ccggtgagcc aaaacgcatc
      361 catgtaactc agaaggaagg tctgcttctg tactgcgccc tccagcgcct tcaatgccat
      421 ttgctctgcc tgaccgggag cataaccgtg ggcaatgaaa tttgcgacaa ctaccttcat
      481 ccgctccatg gtcgccggat cactggtgga tacatgttcg atcaaatgat tgcggtgaaa
      541 accctgccga aggtggatca gcgtggttat gatcgcgatg ccgaaagagc cacccaactg
      601 acgcatcatg ttgttgagcc ccgttccctg tgcgatgtca ctgggtttca ggctcgacaa
      661 tgccagtgtt gtcaaaggca caaagagtaa gctgagtccg atgccacgca ggattagtgg
      721 caggtaaaaa tcactttcac cggaattcag ggtcgatcgt gagagaaagt atgtaaaaag
      781 gaaaaagaac aagaacccga tcgttcccat gatctgcggc ggcacaccgc gttgcaacaa
      841 tttcccaaca aggggcatca tgaagatcgt cgccaatccg cccgggataa gtaacattcc
      901 tgtttgctgt gcagagaaac ccaataggtt ctggcaaaag accggaaaca caaacacaga
      961 tccgaacaat ccgaagccga gaatgaacgt ggtgaacatc ccgatcgcga gtgagcggtt
     1021 cttcagtatc ctcagatcaa cgatcgggtg ctcggtggtg agctcccgac ggatgaaagc
     1081 gacaataccg gatagcgcga tgatgcttaa caacgtgatg tacggcgttt caaaccagtc
     1141 ttcactttct ccgcgttcca aaacggtttg caatgctcca accccgatcg tgaggaagat
     1201 gatcccccac cagtccacgg ccccgatctt gcgttcatat ttcgattccc ggatatacgt
     1261 cagcgccaac agtacggcga taatgcccag cggaatgttg acatagaaca cccagggcca
     1321 ggagaagtgg tcggtgatgt atccaccgag ggtagggccg agcgtaggac caacgactac
     1381 acccaatccg aacaatgcgg tggccatacc catctgtgct ggcggccaag cctccatgag
     1441 aatcgcctgg gaagtaccca gcaaagcgcc gccaccgatt ccctgcaaaa atcgaaagat
     1501 gaccagttcc cagatattat ccgcatttcc gcagaagaag gaggagacag taaagaggat
     1561 gatagagaaa gtgaaatacc gttttcgccc gaatcgtgca ccgagccatc ctgatatcgg
     1621 gagaatgatg acgttagcca ccgcataagc cgttacgacc caaccgacat cctcgagcgt
     1681 cgcgccgagg ttgcccatga tctgtggcag cgagacgttc acgaccgtgg tatcgatcag
     1741 ctcgagcaat gacgcgagga tcaccgtcag cgtgacgatc catttccgaa aaccggtttc
     1801 agccatgatg tgttgagcga tcgattaact ggtgcggaca gacactttca cgctcatacc
     1861 gggacgaagg tgtaccatgg tcccggactt ggcttccag
//
LOCUS       DAOTON010000596         1898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47238_length_1898_cov_3.145958, whole genome shotgun sequence.
ACCESSION   DAOTON010000596 DAOTON010000000
VERSION     DAOTON010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47238_length_1898_cov_3.145958"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..456)
                     /locus_tag="P5292_10805"
     CDS             complement(94..456)
                     /locus_tag="P5292_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39660.1"
                     /translation="MKAKKHYLYVLVFLLHPFLTTALEPREIKADSSATRQPEVEASS
                     VKGKKLFDVAGQEVSAKSTPVRELYLEAPGEVLLLDAEGHILPAGKEVQMVVISGLKP
                     GRYHFRDETGKLRKLRIR"
     gene            848..>1898
                     /gene="thiL"
                     /locus_tag="P5292_10810"
     CDS             848..>1898
                     /gene="thiL"
                     /locus_tag="P5292_10810"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009422542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRS39661.1"
                     /translation="MFQDNTSRTELGQMGEFGFIEHLSRSVKIHHPEESLKGIGDDAA
                     VLDTKGELSVVSTDMLVEGVHFDLAFHPLRHLGYKAVVVNVSDLCAMNADPKQVLVSI
                     AVSNRFSLEALDELYEGMHLACERYKVDLVGGDTTASRSGLVISITILGTAKKEQLVY
                     RNGASAKDLLCVTGDLGGAYIGLQVLEREKKIFLENPGIQPDLSGNEYVIERQLKPEA
                     RRETILKLRELGVHPTSMIDISDGLSSETLHLCRQSGTGCDLYEDKFPIDPQTYDRAR
                     EFGLDPTVCALSGGEDYELLFTIRPDDYEKLREHLDVSIIGHMTDQASGVNLITRSGG
                     SVPITAQGWDALLSQN"
BASE COUNT          482 a          534 c          446 g          436 t
ORIGIN      
        1 cacactacga caggaggtgc gtgatgcgag gtgcgaagtc ccattgaaac caatacctaa
       61 tcactaatta ctaattacca attcccaatt ccctcacctt atcctcaact tcctcaactt
      121 tcccgtttca tcgcgaaagt gatagcgtcc gggttttaat ccggatatca cgaccatctg
      181 tacttctttt cctgctggca ggatatgccc ctccgcgtcg agcaggagaa cttcacccgg
      241 cgcttcaaga tacaattcac gtaccggggt tgattttgca gacacttcct gccctgcaac
      301 atcaaacaac ttcttgcctt taacggacga cgcttcgact tcaggctgcc tggtcgcact
      361 cgaatccgct ttgatttccc tgggctccag agccgttgtc aaaaacggat gcagcaggaa
      421 taccaataca tacaaataat gctttttcgc tttcaacaga tttttcatgc tgcaaagaac
      481 ttcccctaac attacgtcac gttcaaccca cgctgagtaa aaagttaaat tgctgtgaag
      541 gcgatgttag gagggggtct tgaaggttga aggttgaagg ttgaaagtct aaggtgggga
      601 aataaaggga ctctcctcac aggaacttct tcccttccgg aaaaatctgc tgtaaagaca
      661 acacttgaat taattcgtgt taagggatgg tagcccgaga tagccaaccg aaatcgttcg
      721 aacgctaata ccctaaaatg ggccagtcca aatcaaccaa cattcagtcg atcatcccga
      781 aatccaactt tcaacttgca cccttcaact tgcaacccaa taaagttcaa ctaactacct
      841 ttacaacatg ttccaggaca acacctcccg caccgaactc gggcaaatgg gcgaattcgg
      901 ctttattgaa cacctttcac gctcggtcaa aatccatcat ccggaggaat cattaaaagg
      961 catcggcgac gacgcggccg ttttggatac gaagggggag ctgtcggttg tctcaaccga
     1021 tatgctggtc gaaggggtgc acttcgatct tgcctttcac ccgttgcgcc acctgggata
     1081 caaggcggtc gtggtcaatg tatccgacct ctgcgcgatg aatgcggacc ctaaacaggt
     1141 attggtcagc atcgccgtat ccaaccgctt ctcactcgaa gccctggacg aactgtacga
     1201 aggcatgcac cttgcctgcg aacgctataa agtcgacttg gtcgggggcg ataccactgc
     1261 ttcccgttcc ggtcttgtca tcagcatcac catcctgggc accgcgaaaa aagaacaact
     1321 ggtttatcga aacggcgcca gcgcgaaaga cctgctgtgc gtgaccggcg acctcggtgg
     1381 cgcttatatc ggactgcagg tgctcgaacg tgagaagaag atctttctgg aaaatcccgg
     1441 catacaaccc gacctgtcgg gtaacgagta tgtgatcgaa cggcaactca aaccggaagc
     1501 ccgccgggaa acgatactca aacttcggga actcggcgtt catccgacct cgatgatcga
     1561 catctccgat ggcttgtcgt ccgaaaccct gcacctttgc cgtcaatccg gaacgggctg
     1621 cgacttatac gaagacaaat tccccatcga tccgcagacg tacgaccgcg cacgcgagtt
     1681 cggcctcgac ccgaccgtct gcgctttaag tggcggagag gattatgaac ttctgttcac
     1741 cattcgtcct gacgattacg aaaagctccg ggagcacctg gatgtttcga tcatcggtca
     1801 catgaccgat caggcatccg gcgtgaacct gatcacgcgc tccggcggat cggtaccgat
     1861 cactgcgcaa ggttgggacg cgctgctttc gcagaact
//
LOCUS       DAOTON010000597         1897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47278_length_1897_cov_3.509229, whole genome shotgun sequence.
ACCESSION   DAOTON010000597 DAOTON010000000
VERSION     DAOTON010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47278_length_1897_cov_3.509229"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1798
                     /locus_tag="P5292_10815"
     CDS             <1..1798
                     /locus_tag="P5292_10815"
                     /inference="COORDINATES: protein motif:HMM:NF036968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRS39662.1"
                     /translation="STFQYNAIIREVGKVFGLPKEEIDALSETRRHPDTPDHITRRIW
                     KIAQRITDFPRHLSIHAGGVLISEKPVTWYTATVRPPKGFPVTQFSMLEAEDVGLYKY
                     DILSQRGLGHIRDAVELVKKNRGETVDIHDIARFKRDERVRALIREGRCMGCFYVESP
                     AMRMLLKKLRVDTYIQLVAASSIIRPGVARSGMMREYILRTHDPARRQYIHPLMRELM
                     EETYGIMVYQEDVIKVAHHFAGLSLSEADVLRRGMSGKFRSRDEFAKIRDKFFASCAE
                     RGYPDSITTEVWRQIESFAGYSFSKGHSASYAVESFQSLFLKAYYPKEFMVGVINNFG
                     GFYRTEFYVHEARRSGANVHAPCVNRSDYLTNISGDDIHLGFIHLSGLEQSAADALLA
                     ERAANGPFLSLDDFVHRVPIAVEQLRILIRIGAFRFSGKGKKELLWDIPTLLGAEKRT
                     RIVPELFGVEPETFTLPDLYHDPVDDALDEIELLGFPLCDPFSLLRDPLPSPASACDF
                     EQNVGREIAIVGYLVTTKQTTTVKREMMQFGTFLDREGRFFDTTHFPRVTEQFPFRGR
                     GLYLLRGKVDQEFGFCSLTVSGMQKLETKGRR"
BASE COUNT          379 a          617 c          542 g          359 t
ORIGIN      
        1 ttccaccttc cagtacaacg ccatcatccg cgaggtcgga aaggtcttcg gcctgccgaa
       61 agaagagatc gacgcgctct ccgaaacgcg ccgtcatccc gatacacccg accacatcac
      121 gcgccgcatc tggaagatcg cgcagcgcat caccgacttt ccccgccacc tcagcatcca
      181 cgccggcggc gtgctgatct ccgaaaagcc ggtcacctgg tataccgcca ccgtgcgacc
      241 gccgaagggt tttccggtca cgcagttcag catgctcgaa gccgaggatg tcgggctgta
      301 caaatacgac atcctcagcc agcgcggact cggtcacatc cgcgacgcgg tcgagctggt
      361 gaagaagaac cgcggtgaga cggtcgacat ccacgatatc gcccgcttca agcgcgacga
      421 acgggtgcgc gcactcatcc gcgagggccg ctgcatgggc tgcttctacg tcgagtcgcc
      481 cgccatgcgc atgctgctga agaaactgcg ggtcgatacc tacatccaac tggtcgccgc
      541 gagctcgatc atccgcccgg gtgtcgcccg ctcaggcatg atgcgcgagt acatcctccg
      601 gacccacgac cctgcccgcc ggcaatacat ccacccgctc atgcgggagc tgatggagga
      661 gacctacggc atcatggtgt accaggagga tgtcatcaag gtcgcgcatc acttcgccgg
      721 cctctcactc tccgaagccg acgtgctccg tcgcggcatg tcggggaagt tccgctcgcg
      781 cgacgagttc gccaagatcc gcgataagtt cttcgccagt tgcgccgaac gcggctaccc
      841 ggatagcatc accaccgaag tatggcggca gatcgaaagt ttcgccggct actcgttttc
      901 caaaggccat tcggcttcct atgcggtcga gagtttccag agcctgttcc tgaaggccta
      961 ctacccgaag gaattcatgg tcggggtgat caacaacttc ggcgggtttt accggaccga
     1021 gttctacgtc cacgaagcgc gcaggtcggg tgcgaacgtc cacgcgccct gtgtcaatcg
     1081 gagcgactac ctcacgaaca tttcgggcga cgacatccac ctcggcttca tccacctctc
     1141 cggcctggaa caatcggcgg cggacgcgtt actcgccgag cgcgccgcca acggtccctt
     1201 cctgagcctc gacgatttcg tacaccgtgt ccccattgcg gtcgagcagt tgcgcattct
     1261 gatccgtatc ggcgcgttcc gcttttccgg caaggggaag aaggagctgc tgtgggatat
     1321 tcccaccctg ctcggcgcgg aaaaacgtac ccgcatcgtg ccggaactgt tcggggtcga
     1381 accggagacc ttcaccttgc cggacctcta ccacgacccg gtcgacgatg cgctcgacga
     1441 gatcgaactc ctcggctttc cactctgcga tccgttttcg ctcttgcgtg atcccttgcc
     1501 ttcgccggca agtgcctgcg acttcgaaca aaatgtcggt cgggagatcg ccatcgtcgg
     1561 ttacctggtg accaccaagc agaccaccac ggtcaaacgc gagatgatgc agttcggaac
     1621 cttcctcgat cgcgaaggac gcttcttcga cacaacgcac ttcccccgcg tcaccgaaca
     1681 gttccccttc cggggccgcg gactctacct cttacgcgga aaggtcgatc aggaattcgg
     1741 cttctgcagc ctcacggttt ccggcatgca gaagttggaa acgaagggga ggaggtgagg
     1801 ggagggcggt tggcggttgg cagtaagcag taggcggtga gcagtatgca gagaagagta
     1861 gctattagtg aatagcgaat cctgcctgcc agagctt
//
LOCUS       DAOTON010000598         1896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47322_length_1896_cov_2.639326, whole genome shotgun sequence.
ACCESSION   DAOTON010000598 DAOTON010000000
VERSION     DAOTON010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47322_length_1896_cov_2.639326"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="P5292_10820"
     CDS             <1..770
                     /locus_tag="P5292_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRS39663.1"
                     /translation="AQGLAINGMKAMVEMQFSDFVTCGFNQIVNNLAKTHYRWGQNVD
                     VVVRMPTGAGVNAGPFHSQSTEAWFTHTPGLKVVYPAFPDDAKGLLAASIADPNPVMF
                     FEHKALYRSVQGQVPDDYYTLEIGKAKLVRQGNAATIVSYGLAVHWALEYLDQHPEIS
                     VDLIDLRSLLPWDKEMVMESVRKTGKVLVFHEDTHTGGFGGEIASHISEHCFESLDAP
                     VMRLASLDTPVPMNLELEWNFLPKNRLGERLEQLLRY"
     gene            complement(773..>1896)
                     /locus_tag="P5292_10825"
     CDS             complement(773..>1896)
                     /locus_tag="P5292_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRS39664.1"
                     /translation="RIMQLCDSLTDYRFTRSTQLISGASGGMIGAAYYRELFLRQRLG
                     LLSDLQNKKYIDNIGKDLLNPVTFSISVSDLFLNVQKFREGNNRYSKDRAYAFENQLN
                     ENTGSVMVKRLQDYRKPESEAMIPMMVFAPTIVNDGRRLLISPQPISYLTTHRNDSNF
                     NFKTTYDEVEFSDLFREQQAGNIRFSSVLRMNATFPYILPAVSLPSEPMIQVMDAGIR
                     DNTGMKTSLRFLHTFRKWIEENTSGVIFVDIRDSHKERPIEEQPRKTFIENITTPLGN
                     IYGNLLTIQDYNQDESYEYAKAWLTSPFDFIIFELPTKEQEISLSWHLTTRERRSVYN
                     SVYLEQNQVAIARLLELMKPVREQPNKPLLTDVSTTDPK"
BASE COUNT          409 a          477 c          549 g          461 t
ORIGIN      
        1 ccgcccaggg cttagccatc aatggtatga aagccatggt cgaaatgcag ttttccgact
       61 tcgtcacctg cggtttcaac cagattgtga acaacctcgc gaagacgcac tatcgctggg
      121 gacaaaatgt cgatgtagtg gttcgtatgc ccaccggcgc cggcgtgaat gccggaccgt
      181 tccattctca gtcaaccgaa gcctggttca cccatacacc cggtttgaag gtggtctatc
      241 ccgcgttccc ggatgatgcg aaaggattgc tcgcagcatc catcgcggac cccaatccgg
      301 tcatgttctt tgaacacaag gcgctttacc gctctgtcca ggggcaggtt ccggacgatt
      361 attataccct cgagatcgga aaggcaaagt tggtccgtca gggaaatgct gccaccattg
      421 tgagctatgg gctggcggtc cactgggcgc tggaatacct cgatcagcat cccgaaattt
      481 cggttgacct gattgacctg cgctcgctct taccgtggga taaggaaatg gtgatggaga
      541 gcgtgcgtaa gaccggaaaa gtgctggtat tccatgagga cacgcacacg ggaggattcg
      601 ggggagagat cgcttcgcat atttccgagc attgctttga atccctggac gcgccggtga
      661 tgcgactggc cagcctggat acaccggttc ccatgaacct ggaattggaa tggaattttc
      721 ttccgaagaa ccgcctcgga gaacgtctgg agcaactgct ccgttactga tattactttg
      781 gatcggtcgt agaaacgtcc gtgagtaacg gcttgttcgg ctgttcgcgt accggcttca
      841 tcagctcgag taaacgggca atggccacct ggttctgctc gaggtaaacc gaattgtaaa
      901 cggaccggcg ttcgcgggtc gtcagatgcc agctcagcga aatctcctgt tccttggtcg
      961 gtagttcgaa gatgatgaaa tcgaacgggg aggtcagcca ggccttggcg tattcatacg
     1021 actcatcctg gttgtaatcc tgaatggtca gcaggttgcc gtagatgttg ccgagcggcg
     1081 tcgtgatatt ctcgatgaag gtctttcgcg gttgctcttc gatgggacgc tccttgtgag
     1141 aatcacggat atcgacaaag atcaccccgc tggtgttttc ctcaatccat ttgcgaaagg
     1201 tatggaggaa acgcaacgag gttttcattc cggtattgtc ccggatgccg gcatccatca
     1261 cctggatcat cggctcgctg ggtaaactga cagccggcag gatgtaaggg aaggtcgcgt
     1321 tcatccgcag cacactgctg aaccggatgt ttcccgcctg ttgttccctg aagaggtcgg
     1381 agaattccac ttcgtcgtaa gtggtcttga aattgaaatt gctgtcgttc ctgtgggtgg
     1441 taaggtagga gatgggttgg ggtgagatca ggaggcgacg gccgtcgttg acaatggtcg
     1501 gcgcgaagac catcatcggg atcatggctt ccgattccgg tttccggtaa tcctgcagac
     1561 gcttgaccat gacactgccg gtattttcat tgagttggtt ctcgaaagca taggcccgat
     1621 ccttcgagta tcggttattg ccttcccgga acttttggac gttgaggaac agatcgctca
     1681 cggaaatact gaacgtgacc ggattcagca gatctttccc gatgttatca atgtatttct
     1741 tgttctgaag gtcggagagc agtccgagtc gctggcgcag gaacaactcc cggtagtacg
     1801 ccgcgccgat cattcccccc gaagccccgc tgatgagttg ggtggaacgt gtgaagcggt
     1861 agtcggtcag actgtcgcaa agttgcatga tccgaa
//
LOCUS       DAOTON010000599         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47352_length_1895_cov_3.135326, whole genome shotgun sequence.
ACCESSION   DAOTON010000599 DAOTON010000000
VERSION     DAOTON010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47352_length_1895_cov_3.135326"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1355)
                     /locus_tag="P5292_10830"
     CDS             complement(51..1355)
                     /locus_tag="P5292_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRS39665.1"
                     /translation="MSQRRIIRLPGVPDRAELLRRLSDEPVLMLLDSNKALQEQPDRF
                     SRYDLLLAFGVDEQVAAGERPFERLQERIATTGDWWFGHFSYELKNRIERLKSEHPDP
                     IGFPELFFFRPRVVLVVRGGEAWLEVSGLMEESAAESIVRRILDPGNESPAIATRVLI
                     EPTVSRENYGYNFQKLQEHIARGDIYEVNYCVPFEGIAVSFDPISTYSRLTSLSPMPF
                     SGYYRENDRHLLCASPERFLCKRGNQLHSQPIKGTARRHSDPEADRAVRVALANDPKE
                     QSENVMIVDLVRNDLSRHAAKGSVHVEELFGVYSFRNLHQLISTVSAELREGHSSIAA
                     LRDCFPMGSMTGAPKIRAMELIEACENRKRGLYSGAIGYFAPEGDFDFNVVIRSIQYN
                     AVNRLVSYTAGSAITARAEADREYDECLLKAESMRLAVGSRQ"
     gene            complement(1386..>1895)
                     /locus_tag="P5292_10835"
     CDS             complement(1386..>1895)
                     /locus_tag="P5292_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39666.1"
                     /translation="DTLACVGCITVYPRPVRPLITVSNDTLFVDPSYSNFQWYLSGQP
                     IAGATQSFYVMQVGGVYSVTSDDPNGCPAPISNIDIVLGVPNAPATMPVVVSLSSGWI
                     AVREIPAAFQDGHLRVYNLLGRLVREERISGTTALFDLSAHPDHWLLIELIGKNDAVY
                     RVRLVRTGW"
BASE COUNT          421 a          567 c          510 g          397 t
ORIGIN      
        1 cctactgccc tccgccaacc gcctactgcc aaccgcctac cgcctactgc ctactgccta
       61 ctgcctaccg ccaaccgcat actctccgcc ttcaacaagc actcatcgta ctcgcgatct
      121 gcttcggcgc gcgcggttat cgcgcttccc gctgtatacg aaaccaatcg attcacggca
      181 ttgtactgga tgctccggat gaccacattg aaatcgaaat caccctcagg tgcaaagtag
      241 ccgatcgctc cggagtacag tccgcgcttg cggttctcgc aggcttcgat gagttccatc
      301 gcgcggatct tcggcgcgcc ggtcatggat cccataggaa aacaatcgcg aagagcggca
      361 atggacgaat gcccttcgcg caactccgcg ctcacggtgg agatgagttg gtgcaggttc
      421 cggaagctgt aaacgccaaa caactcctcc acatgtacgc tgcctttcgc ggcatggcgg
      481 gaaaggtcgt tgcgtacgag gtcgacgatc atgacatttt ccgactgttc tttcggatcg
      541 ttcgccaatg cgactcgtac cgcgcggtct gcttccgggt cgctgtgcct gcgtgccgtt
      601 cctttgatcg gttgcgaatg caactgattg ccgcgcttac acaggaaccg ctccggactg
      661 gcacacagca ggtgccggtc gttctcccgg tagtatccgg aaaacggcat cggggagagg
      721 gaggtcagtc gggaataggt gctgatcggg tcaaaggata ccgcgattcc ttcgaacggg
      781 acgcaatagt tgacttcata aatatcgccg cgcgcgatgt gctcctgcaa tttctggaag
      841 ttgtatccat agttttccct ggatacggtg ggctcgatca ggacgcgtgt ggcaatagcc
      901 ggagattcgt tgccgggatc caggatcctt cgtacaatcg attcggcagc cgattcctcc
      961 atcagaccgg acacttctag ccaggcttcg ccgcctcgta ctaccagcac tacgcgggga
     1021 cgaaagaaga aaagttccgg aaaccctatc ggatccggat gttccgattt caatcgttcg
     1081 atccggttct tcaactcgta ggaaaaatgc ccgaaccacc agtcgcctgt agtcgcaata
     1141 cgctcctgca agcgctcgaa cggacgctca ccggccgcta cctgttcgtc tacgccaaag
     1201 gctagcaaaa gatcgtaccg gctgaatcgg tctggctgct cctgcaaggc tttattcgaa
     1261 tcgagcagca tcaataccgg ttcgtcggat aatcgacgca gcagttcagc tcgatccgga
     1321 actccgggaa ggcgaatgat cctgcgctgc gacatgccgc aaagatagca ggacgacgct
     1381 tccggctacc agcctgttcg caccaaacgt acccggtaaa ccgcgtcgtt ctttccgatc
     1441 aactcgatta gcagccaatg atccggatgc gccgataagt cgaacagcgc ggtcgttccc
     1501 gatattcgct cttcccgaac caggcggccc aacagattgt acacccgaag atgtccgtcc
     1561 tggaatgcag ccggtatttc ccgcacagcg atccaaccac tactgaggct gacaacaacc
     1621 ggcattgtgg ccggagcatt tggtacaccc aagacgatgt cgatgttgga aatcggggca
     1681 gggcatccgt tcggatcgtc cgaggtaaca gaatacactc ctccgacttg catcacatag
     1741 aacgactgcg tggcgcctgc gatcggttgc ccggaaagat accattggaa attgctgtag
     1801 gaggggtcaa caaagagggt gtcgttcgag acggtgatca gcggacgaac gggtcggggg
     1861 tacaccgtga tacaacccac acacgccagt gtatc
//
LOCUS       DAOTON010000600         1894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47393_length_1894_cov_2.740620, whole genome shotgun sequence.
ACCESSION   DAOTON010000600 DAOTON010000000
VERSION     DAOTON010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47393_length_1894_cov_2.740620"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..840
                     /locus_tag="P5292_10840"
     CDS             76..840
                     /locus_tag="P5292_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013071964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HRS39667.1"
                     /translation="MSAAELRNFLDEMTDRYNRPGFIETDPVSIPHRYRNKEDIEIAG
                     FLAGTIAWGQRPVILKNASRLLGWMDDSPAAFVRGFSEKDLKPFRGFVHRTFHPDDCL
                     YFLRALQRAYRENDGLQGLFTPAYPDAPLRDTINSARRRFFEEPGPARSHKHFSDPVK
                     GSAAKRINMFLRWMVRHDERGVDFGIWKQLQPASLICPLDVHSARVGRKLGLLKRKSN
                     DWQAAEELTAALRKLCPEDPVKYDYALFGLGVFERF"
     gene            859..>1894
                     /locus_tag="P5292_10845"
     CDS             859..>1894
                     /locus_tag="P5292_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HRS39668.1"
                     /translation="MQFLNPQFLFAFFALAIPILVHLFNFRRYKKVLFTNVRFLQEIR
                     QDTQNRSRLRNLLILASRLLAVTFLVLAFAQPYLPASTAAKRAAERRVAIWIDNSFSM
                     EAQGSYGTLLDEARKKAREIALAFSPSDKFLLVTNDFEARHQHLVNRDEALQWIDAVQ
                     AGPAARSAGEVLLRARDGLKSGNLPASVSFDAYLLSDFQRRIADMASAPADSSVKVHL
                     LALKANATANVFIDTCYLESPVVQLGSPNELIVSLRNSGEKPVENIPVRLTINGYQKA
                     LASVPLEAGAQQEVRLAFTIQNPGWQEAIVSLTDYPVTFDDTFHFTFRVRTDVPIVAI
                     NGSAGASPYLD"
BASE COUNT          362 a          622 c          508 g          402 t
ORIGIN      
        1 tgttctgcat cgcgctcgct ctcgtccgtc tcctctattg agcgcaatgc agtagcttcg
       61 ccgtcgatgg cacgtctgtc ggcggcggag ttacggaact ttctggacga aatgaccgac
      121 cggtataacc ggcccggttt cattgagacc gatcccgttt ccattccgca tcgttaccgc
      181 aacaaagaag atattgaaat cgccggtttc ctcgccggga ccatcgcctg gggacaacga
      241 ccggtgatcc tgaagaatgc ctcccgcctg cttggctgga tggacgactc acccgccgct
      301 ttcgtgcggg gcttttccga aaaggacctg aaaccgttcc ggggttttgt ccaccgaacc
      361 tttcacccgg acgattgcct ctactttctc cgcgccctgc aacgggccta tcgcgaaaac
      421 gatgggctcc agggtctatt taccccggcc taccccgatg ctccgctgcg cgacaccatc
      481 aacagcgccc gccgacgctt tttcgaagag cccggacctg cccgctccca caagcatttt
      541 tctgatccgg tcaaaggctc cgccgccaag cggatcaaca tgttcctgcg ctggatggta
      601 cgccacgacg agcgcggtgt ggacttcggt atctggaaac aactgcaacc cgcttcgctg
      661 atctgcccgc tcgatgtcca ttcggcccgg gtcggccgga aactcggcct gttgaaacgc
      721 aaatccaacg actggcaggc cgccgaagaa ctgaccgccg ccctgcggaa actgtgcccg
      781 gaggatcccg tgaagtacga ctatgccctg ttcgggctgg gggtattcga acgcttctga
      841 ttgcgtatct ttgacgcaat gcagtttttg aacccgcagt tcctcttcgc ctttttcgcc
      901 ctggcgattc cgatcctggt tcacctcttc aatttccggc gttacaagaa ggtgttgttc
      961 accaacgtcc gtttcctgca ggagatccgg caggacaccc agaaccggtc ccggctgcgc
     1021 aacttgctca tcctggcttc ccgcctgctt gccgttacgt tcctggtcct cgcctttgcc
     1081 cagccctacc tgccggccag taccgccgcc aaacgtgcgg ctgaacgccg ggtagccatc
     1141 tggatcgaca actccttcag catggaggcc cagggtagtt acggaacctt gctcgatgaa
     1201 gccaggaaga aagcgcgtga aatcgcactg gccttctcac cttccgacaa attcctgctg
     1261 gtcaccaacg atttcgaagc ccggcaccag cacctggtca accgggacga agccctgcag
     1321 tggatcgacg cggtacaggc gggtccggct gcacgctccg ccggtgaagt cctgctgcgg
     1381 gcacgcgacg gactcaaatc gggcaacctg ccggccagtg tttccttcga cgcttacctg
     1441 ttatcggatt tccagcgacg catcgccgat atggcttccg ccccggccga ttccagcgtg
     1501 aaggtccacc tgctcgccct gaaggcgaac gcaacggcga atgtcttcat cgatacgtgt
     1561 tacctcgagt cgccggtggt gcaactgggc agtcccaatg aactgatcgt gagcctgcgc
     1621 aacagcggtg aaaaaccggt ggagaacatt ccggtccggc ttaccatcaa cggttaccaa
     1681 aaggcactcg ccagcgttcc gctggaagcc ggcgcccaac aagaagtccg cctggccttc
     1741 acgattcaaa atcctggctg gcaggaagcg atcgtgagcc tgacggatta cccggtcacc
     1801 ttcgacgata cctttcactt caccttccgt gttcgaacgg atgttcccat cgttgccatc
     1861 aacggcagcg cgggcgcgag tccttacctg gatg
//
LOCUS       DAOTON010000601         1891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47471_length_1891_cov_3.988562, whole genome shotgun sequence.
ACCESSION   DAOTON010000601 DAOTON010000000
VERSION     DAOTON010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47471_length_1891_cov_3.988562"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="P5292_10850"
     CDS             <1..244
                     /locus_tag="P5292_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRS39669.1"
                     /translation="VIPAEVIRATTEEKEYQISMLKDLHARNEKKAGDQLKSLQLAAV
                     QNKNMFSELMEAVKYCSLGQITKSLFAVGGQYRRNM"
     gene            241..1458
                     /locus_tag="P5292_10855"
     CDS             241..1458
                     /locus_tag="P5292_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39670.1"
                     /translation="MIMVAGLQNDSFFSRMLTRRWWVTIVLVLFSAHVYSQDIVRYKD
                     GGIERGAVQQSGFGFIRFSTDPQTGVSRRISKQDLTSLTYASGESIYFNAYTSDTPRT
                     RKVIIHLRSEETVTGRLIRQTNDSLIVRAGKGRREEVLVLRTDSLRKLEFLSGEREYY
                     SPTYREPAVHFLPYRRLQLGLLLGGGGYSEQTDVVDAYEGAGFGLLTTNSNGMPTAAK
                     GLEFKPTPTQIKLQAAYRFRRNLQLSVQLPLQRKTFSLSMGRPVGSLNPLPLAYKFHL
                     TQAAALFDFVFLPYPRKQRTAFELQVSCGMGFDVVEEEFELRSDSGYFWSSSRSSGGM
                     RGIFGFTSRLHLSRIVSIVPLQLQFDIPVLRPAFEESYVRYPDGLVRVSSYNYYPFGV
                     AVHWGVTFHLLSQ"
     gene            complement(1471..>1891)
                     /locus_tag="P5292_10860"
     CDS             complement(1471..>1891)
                     /locus_tag="P5292_10860"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HRS39671.1"
                     /translation="RWAKQMGYCLFTFKTKDEQERPYYHTNVVLSVGEHWAIWCPDAM
                     PFEPEKKELEHLLRQLGKEQITITRKQLSAFCANVLELENESGSKLLAVSSTAWDAFT
                     PQQQHQLRRYCTPLIFEIPVIEKTGGGGIRCMLAELA"
BASE COUNT          409 a          478 c          526 g          478 t
ORIGIN      
        1 cgtcataccg gctgaagtga tccgggcaac cacggaagag aaggagtacc agatcagcat
       61 gttgaaagac ctccacgcgc gaaatgaaaa gaaggcgggc gatcaactga agagcctgca
      121 actggcggcc gttcagaaca agaacatgtt ctccgagctg atggaagcgg tgaagtattg
      181 ttccctggga cagatcacca aatccctctt cgccgtcggc ggtcagtacc ggcgcaacat
      241 gtgatcatgg ttgctggctt gcagaacgat agcttcttct cgcgcatgct gaccaggcgt
      301 tggtgggtaa cgattgtgtt ggttctgttc agtgcccacg tatacagcca ggatatcgtg
      361 cggtacaaag acggcggtat cgaacgcggg gccgtacagc aatccggatt tggtttcatt
      421 cgtttcagta cggatcctca gaccggggtc tcgcgaagga tctccaagca ggatcttacc
      481 tcgttaacct atgcttccgg cgagagcatt tatttcaatg cctacacaag cgacacaccc
      541 cgtacacgga aggtgatcat tcacctgcgg agcgaggaga cggttaccgg ccgactgatc
      601 cggcagacca acgacagcct gatcgtacgg gcagggaagg gtagaaggga agaggtgttg
      661 gtgttgcgaa cggacagctt gcgaaagctc gagttcctga gcggcgaacg cgagtattat
      721 tcgccgacgt atcgcgagcc ggcggttcat tttcttccct accggcgttt gcaattgggt
      781 ttgctgctgg gcggcggcgg gtattcagag caaacggatg tggtagatgc ctatgagggt
      841 gcgggttttg gtctgttgac tacgaactcc aacgggatgc ccacggcagc caagggactt
      901 gagttcaagc cgacaccgac ccagatcaag ttgcaggcag cgtatcgatt tcgtcgcaat
      961 ctgcaattgt ccgtccagct gccccttcag cgcaagacgt tttcgctttc gatgggtcga
     1021 cctgtcggca gtcttaatcc attaccgtta gcatacaaat ttcacctcac gcaggcagca
     1081 gcattatttg atttcgtctt tttgccctat ccgcgaaaac aacgaacggc gtttgaactg
     1141 caggtttctt gcggaatggg tttcgacgtc gtggaggaag aattcgaatt gcgatccgat
     1201 agcggatatt tttggagttc ttccaggtcg agcggtggta tgcgtggtat cttcggattc
     1261 acctcccggc tgcaccttag ccggatcgtt tcgatcgttc cgttacagct tcagtttgat
     1321 ataccggtac tacgtccggc gtttgaagaa tcctatgttc ggtacccgga cgggttggtg
     1381 cgcgtcagct cgtataatta ctatccattt ggtgtagccg tccattgggg tgttacgttt
     1441 cacttgcttt cgcaatgaat gcgtttctgc ttaagccaat tctgccagca tacagcgaat
     1501 gcctccgcct cctgttttct cgatgacagg aatttcgaag atcagcggag tacaatacct
     1561 gcgaagctga tgctgttgtt gaggcgtaaa tgcatcccag gcggtcgagc ttactgccag
     1621 cagtttcgag ccgctttcat tttccaattc caggacgttg gcgcagaatg ccgagagctg
     1681 ctttcgcgtg atggtgatct gttctttgcc caactgacgg aggagatgtt ccaattcctt
     1741 tttctccggt tcgaagggca ttgcatccgg acaccagatc gcccagtgct caccgaccga
     1801 tagtaccaca ttggtgtggt agtaaggtcg ttcctgttca tccttcgttt taaaggtaaa
     1861 caggcaatag cccatttgct ttgcccaccg t
//
LOCUS       DAOTON010000602         1891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47480_length_1891_cov_3.127451, whole genome shotgun sequence.
ACCESSION   DAOTON010000602 DAOTON010000000
VERSION     DAOTON010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47480_length_1891_cov_3.127451"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="P5292_10865"
     CDS             complement(<1..223)
                     /locus_tag="P5292_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRS39672.1"
                     /translation="MKYDRSGLSDDQLRRIYKAILKPRMIEEKMLVLLRQGKLAKWFS
                     GIGQEAIAVGCGLALDRDEYILPMHRNLGV"
     gene            371..529
                     /locus_tag="P5292_10870"
     CDS             371..529
                     /locus_tag="P5292_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39673.1"
                     /translation="MEWQSLVAYVLLLLAAGYLLQKLVGTFSGKKTSGCSACDPGSMK
                     SVENKKGA"
     gene            complement(560..853)
                     /locus_tag="P5292_10875"
     CDS             complement(560..853)
                     /locus_tag="P5292_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39674.1"
                     /translation="MFPKEKALPLRPFNQKTIQKTMKKVLSTLFVAGMIALVACGPSA
                     EEKAAQEKAAQDSIAAAEAAAKAAAEQAVMQDSAAAAAATVVDSAAAAVESAK"
     gene            complement(924..1274)
                     /locus_tag="P5292_10880"
     CDS             complement(924..1274)
                     /locus_tag="P5292_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39675.1"
                     /translation="MKKVFALLLSAGMIATVACGPSAEEKAAAEKARQDSINAVMEKA
                     AADSAAAAQAALEQARQDSINAAMEQAKADSIAKAEADKAKAKPKPKPKPKEEPKQAP
                     TGQPKVGMKKPGAK"
     gene            1421..>1891
                     /locus_tag="P5292_10885"
     CDS             1421..>1891
                     /locus_tag="P5292_10885"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HRS39676.1"
                     /translation="MKKSILYSAFAILGFGTLVLTGCSKDEDTTAPSLSLTGGTSITV
                     SLPTTAGGAGTWSDPGFSALDDEDGNITSSVTVTGASAVNLNRKGSYTVSYSVSDKAG
                     NSTSVDRVINVVNDAEVFAGSYATCTDSSTTFALVANFAASCSTSDTINNLVKVV"
BASE COUNT          427 a          497 c          483 g          484 t
ORIGIN      
        1 agacaccgag attccggtgc atgggcagga tgtattcatc gcgatccagg gcaaggccgc
       61 acccaacggc gatagcttcc tgaccgatac cggagaacca tttcgccagt ttcccctggc
      121 gcaggagcac gagcattttc tcctcgatca tgcgaggctt caggatagct ttatagatac
      181 gccggagttg gtcgtcggag agtccggaac ggtcgtactt catcataggt gaaatcgcgg
      241 ttggcgctgc aaaattaccg aatcagaacg ggctgctgcc ttggggccgg caggttgatt
      301 tacagacttt ccaacaaatc agcccgacta ccctgaacga aaagcaagcc cgcattcgta
      361 tcttagcgga atggaatggc agtctttggt cgcttatgtg ctcttgctgc ttgcggcggg
      421 ttacctgttg cagaaattgg ttggaacatt ctccgggaaa aagacttccg gatgctccgc
      481 ctgtgacccc ggatcgatga aaagtgtaga aaataaaaaa ggagcctgag gctccttttt
      541 tatttttttc tgcgcggaat tacttggcag actcaacagc agcagcagca gagtcaacaa
      601 ccgttgcagc agcagcggca gcagaatcct gcataacagc ctgctcggca gcagccttag
      661 cggcagcttc agcagcagcg atcgaatctt gagcagcttt ttcctgagca gctttttctt
      721 ctgcgctcgg accacaagct acgagagcga tcattccggc gacgaacaag gtagagagaa
      781 cctttttcat tgttttttgg atggtttttt gattgaatgg gcgcaaaggt agggcttttt
      841 ccttaggaaa caaggcctta ggttaaaaaa attaacaggg gaacgttaat atccgctccc
      901 ctgttttatg ctaaaaagcc agattacttg gctcccggct tcttcatgcc caccttgggc
      961 tgtccggtcg gggcctgctt cggctcttct ttcggcttgg gcttgggttt cggctttgct
     1021 ttcgccttgt ccgcttcagc tttagcgatg gaatcggctt tagcctgctc catagccgcg
     1081 ttgatcgaat cctgacgagc ctgctccagg gcagcctggg cagctgctgc ggaatcagct
     1141 gcagcctttt ccattacagc attgattgaa tcctggcggg ctttctcagc cgcagccttt
     1201 tcttcagcgc tcgggccgca cgcgaccgta gcgatcatac cggctgagag aagaagggcg
     1261 aatactttct tcatgatggt tgttggttaa tcgtttgatt tttgattgac ggcaaagata
     1321 tactgaaagg aatgaaaatt gtttcggcca ggcaaaagaa agataattcc gctatttttg
     1381 cggtaccaaa cgcaacgtaa cttaaccaat caatcctcca atgaaaaaat caattctgta
     1441 ttccgctttt gcgattctcg gatttggcac gctggtcctg acgggctgtt ccaaggacga
     1501 agacaccact gctccttctc ttagcctgac cggcggtacc agcatcacgg tcagccttcc
     1561 taccacagca ggtggcgccg gcacctggtc ggatccgggt ttcagcgctt tggatgacga
     1621 agatggcaac atcaccagct ccgttaccgt taccggcgct tctgctgtca acctgaaccg
     1681 caaaggctcc tacactgttt cctactctgt aagtgacaag gcaggcaact cgacatctgt
     1741 cgaccgcgtg attaacgtgg tgaacgatgc ggaagtattc gctggctctt acgccacctg
     1801 taccgattca tccacgactt ttgccctcgt tgccaatttt gcagcctcct gttccacctc
     1861 agatacgatc aataacctgg tcaaagtggt g
//
LOCUS       DAOTON010000603         1889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47548_length_1889_cov_3.081243, whole genome shotgun sequence.
ACCESSION   DAOTON010000603 DAOTON010000000
VERSION     DAOTON010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47548_length_1889_cov_3.081243"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="P5292_10890"
     CDS             complement(<1..228)
                     /locus_tag="P5292_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39677.1"
                     /translation="MSVIRDQHIDFILQDLHRRGIVREDLRDNLLDHLCCLLEAEEWE
                     EDAFGKTYERIIRRFYKHRLSELEEETTNLLT"
     gene            complement(230..568)
                     /locus_tag="P5292_10895"
     CDS             complement(230..568)
                     /locus_tag="P5292_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRS39678.1"
                     /translation="MYSSELIKGTLKTIILKLLKENKRMYGYEITQRVKEMTGGKIQL
                     TEGALYPTLHALEADGMLTTETEYLGKRVRKYYSLSKAGDKLSEEKVNEFADFMNTMK
                     FLLGINLSNA"
     gene            complement(764..>1889)
                     /locus_tag="P5292_10900"
     CDS             complement(764..>1889)
                     /locus_tag="P5292_10900"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRS39679.1"
                     /translation="AVCWSVSTGGPIYSSPVIDQQFLVFGSTDSSLYVVDHRSGKVAW
                     TYKTGGAIRCTPYVRNGIAYFLSDDGKVYAVSIRSHTEQWVYRTEGEARYPLYGYADY
                     FHSSPVADDSTLYFGSGDGHVYALRLSDGSLRWKFKTDAVVHGTGVISEGTLYIGSFD
                     GNVYALATADGGLRWKFKTVGQTYFPRGEVQGTVACFGDRLIVGARDYNLYCLDAEGG
                     TGLWNRQFRKGWAMGVPVIRDSIVYVGTSDDRLLLALDPFSGQAHWEAAVNFNVFGGA
                     AFGDSVLYIGNLRGELLALDVETGKMRWKFTTPGGKEQWRKYLTASGEYRPDIQSIIQ
                     KGEDFVTMYHSMGAIFTTPTVSEGRIFFTSTDGSLYCLQP"
BASE COUNT          467 a          527 c          433 g          462 t
ORIGIN      
        1 ggttaaaaga ttcgttgttt cttcttcgag ctcggataag cggtgcttat agaaccgtcg
       61 tattattcgt tcataggtct tgccaaatgc gtcctcctcc cactcttccg cctccaacag
      121 gcaacacaga tggtccaaca ggttatcacg caagtcctcc cgaacaatgc cacgacggtg
      181 gagatcctga aggatgaagt cgatatgctg atcgcggatg accgacatgt caggcgttgc
      241 tgaggttgat gccaagcaga aacttcatcg tgttcatgaa gtcggcgaac tcgttgactt
      301 tctcttccga caacttatca ccggccttac tgagcgagta atacttgcga acgcgtttgc
      361 cgagatactc ggtctctgtc gtgagcatcc catccgcttc cagcgcgtgc agggtcggat
      421 acaacgcgcc ttccgtgagc tggatcttgc ctcccgtcat ttccttcacc cgctgggtga
      481 tctcgtatcc gtacatgcgc ttgttctctt tcagcaactt cagaatgatc gtcttcaggg
      541 tgcccttgat gagttcggag gaatagatca tgattacctt ggtgtaatat atattggaca
      601 aatatacatc tgtttcttat gtatgtcaag agcaatgcgc ctatatttat ataagtaact
      661 gatatttaat taaataaatt gaatatagat ccttccttgg aaaccgtagc tggggtccaa
      721 accggctttc acaggttcaa ttctatagga aaatggttag ccatcagggt tgcaggcagt
      781 ataaactgcc atccgtactg gtgaaaaaga tgcgcccttc cgatacagtt ggcgtggtaa
      841 aaatggctcc catcgaatga tacatggtca cgaagtcctc gcctttttga atgatgctct
      901 gaatgtcggg acggtattca ccggaagctg tcaggtattt ccgccactgc tcctttccgc
      961 caggcgtcgt gaacttccat ctcatcttac cggtttccac atccagtgcc aacaactctc
     1021 cgcgcaggtt gccgatgtac aacactgaat caccgaacgc cgcgccaccg aatacattga
     1081 agttgaccgc agcctcccaa tgcgcttgcc cgctgaaagg atcgagcgcc aggagtaacc
     1141 gatcatcact cgttccaaca tacacgatcg agtctcggat gacgggaaca cccattgccc
     1201 atccttttct gaactgccgg ttccacaaac ctgtcccccc ctccgcatcc aggcaataga
     1261 ggttgtaatc acgagcgccg acaataagac gatcaccgaa acaggccacc gttccctgga
     1321 cttctcctct cggaaaatag gtctggccca cggtcttgaa cttccagcgc agaccgccgt
     1381 cggctgtcgc cagggcatat acattaccat cgaatgatcc gatatacaat gttccttcgc
     1441 tgatcacgcc ggttccgtgt acaaccgcat ccgttttgaa cttccaacga agactgccat
     1501 cactcagccg tagcgcatag acgtggccgt cacccgatcc gaagtatagg gtactgtcat
     1561 ccgcgaccgg agaggaatgg aaataatccg cataaccata gagcggataa cgcgcttctc
     1621 cttctgtacg atagacccac tgttcggtat gcgatcggat cgaaacggca tacacctttc
     1681 cgtcatcgga taagaaataa gcgataccgt tccgaacgta cggagtacaa cgaatagcgc
     1741 ctcctgtctt gtaggtccag gcaacctttc ccgagcgatg gtccacgacg tacaaactgg
     1801 aatccgtact gccgaatacc aagaattgct gatcaatgac cggagatgaa tagatcggtc
     1861 cgccggtgct caccgaccaa cagaccgca
//
LOCUS       DAOTON010000604         1889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47552_length_1889_cov_2.878408, whole genome shotgun sequence.
ACCESSION   DAOTON010000604 DAOTON010000000
VERSION     DAOTON010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47552_length_1889_cov_2.878408"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="P5292_10905"
     CDS             <1..784
                     /locus_tag="P5292_10905"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRS39680.1"
                     /translation="AATVVANEYQGKFPRTFDELRSLPGIGDYTAAAIASFAFDQAVA
                     VVDGNVFRFLSRCFGIDTPIDSPRAKREFRELAESLMEGHAPHLFNQAIMEFGAISCK
                     SAQPLCASCPFRDTCHAFQRDLITKLPVKAKKQKVRDRYFHYLILNTPKGVYLRQRAT
                     GDIWAKLYEFPLIETDQPTEPASLMRSSAWKRILGKQPIHIRQISREYKHLLSHQRIH
                     ARFYLLDGKVKPEQDWILADKAKRKRLAVSRLVETGLEQAGF"
     gene            complement(789..1316)
                     /locus_tag="P5292_10910"
     CDS             complement(789..1316)
                     /locus_tag="P5292_10910"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HRS39681.1"
                     /translation="MGILSGDPSYKGRPRLSLESGPLDRILEVSGWIALAVLWTYTVS
                     SVLQAPETVPTHFDGSGSPNDFGSRWSLLILPAIGTFVFLVMTVIGRFPHLFNYGLKI
                     TQDNAAFQYRLATRFLRVLKLSILVVLGLVVRLSIRAAAQSSGPPDSATLFIVLALLL
                     LPTAGYIFLSLRNKG"
     gene            complement(1339..1803)
                     /locus_tag="P5292_10915"
     CDS             complement(1339..1803)
                     /locus_tag="P5292_10915"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HRS39682.1"
                     /translation="MPTRHVLSTFLLSLLLACNWREPRSVPEWIIGEWEMADAEGRLV
                     ERWTKVNDTLMKGFSYQVVQGDTVFSETMECYLSGTEVFYVPTVSGQNDGKSVVFRMA
                     DHAGDSVSFVNPEHDFPSRIVYRRVGRDSLVASIEGMIEGTFRRQELPMRKL"
BASE COUNT          453 a          543 c          518 g          375 t
ORIGIN      
        1 ggcggccacg gttgtggcga atgaatacca gggcaagttc cctcgcacct tcgacgaact
       61 gcgcagctta ccgggtatcg gcgactacac cgctgcggcc atcgcttcct ttgcctttga
      121 ccaggcagtc gccgtggtcg acggtaacgt gttccgcttt ttgtcgcgtt gcttcggtat
      181 tgatacgccg atcgactccc cacgcgcgaa gcgggaattc cgggaactcg cggagtcgtt
      241 gatggaaggc catgcgcctc acctgttcaa ccaggcgatc atggagttcg gggcgatcag
      301 ttgcaagtcg gcacagccgt tgtgtgcaag ctgtcctttc cgggatacct gtcatgcctt
      361 tcaacgtgat ctgatcacca agcttcccgt aaaagccaaa aagcaaaagg tccgtgaccg
      421 ctacttccac tatctgattc tcaacacgcc gaaaggcgtg tacttgcggc aacgagctac
      481 cggcgacatc tgggcgaagc tctacgaatt tccgctcatt gaaacggatc agcccactga
      541 accggcatca ctcatgcgtt cgtccgcctg gaaacgcatc ctgggaaagc aaccgatcca
      601 tatccgacag atcagtcggg aatacaagca cctgctgagt caccagcgca tccatgcccg
      661 cttctatcta ctggatggca aggtgaagcc ggagcaagat tggatactcg ccgataaggc
      721 gaaacgcaaa cggctggccg tatcgcgcct ggtcgagacc ggcttagagc aggcaggctt
      781 ttgagggatc agcctttgtt gcgcagcgag agaaaaatgt agccggctgt cggcaacaac
      841 agcaatgcga gcacgatgaa caaggtagcg ctgtccggcg ggccggatga ttgcgccgca
      901 gcccggatcg aaagacggac aacaagtccc agcacaacca ggatcgacaa tttcagcaca
      961 cgcagaaaac gcgttgccaa ccggtattga aaagccgcat tgtcctgcgt gatcttcaaa
     1021 ccgtagttga acaggtgcgg gaagcgtccg atcaccgtca tcaccagaaa aacaaacgtc
     1081 ccgatcgcgg gaaggatcag taacgaccat cggcttccga aatcattggg agatccgctg
     1141 ccgtcgaaat gggtgggcac ggtttccggg gcctggagca ccgaagaaac ggtgtacgtc
     1201 cacagcaccg ccagtgcaat ccagcctgaa acttcgagga tacgatccag cggtccgctt
     1261 tccaacgaaa gtcggggcct gcctttgtag gaaggatcac cactcaagat gcccataggt
     1321 gtcggttaaa ctgcgaagtt acaatttccg catcgggagt tcctgccgcc gaaaggtccc
     1381 ttcgatcatg ccctcaatgg acgcgaccag ggaatcgcgg ccgacgcgcc gataaacaat
     1441 gcggctcggg aaatcgtgct ccgggttcac aaagctcacg ctatcaccgg catgatcggc
     1501 catccggaac acaaccgact tcccatcgtt ctgtccgcta acggtcggca cgtagaaaac
     1561 ctcggtacca ctgagatagc actccatcgt ttccgaaaat accgtatcgc cctgcaccac
     1621 ctggtagctg aatcccttca tcagcgtatc gttcaccttc gtccaccgct ccaccagccg
     1681 cccctcagca tcggccattt cccattcacc aatgatccac tccggaacag accggggttc
     1741 tctccagttg caggcaagga gcagggagag gagaaaggtg gaaaggacat ggcgggtggg
     1801 catgggtggt ggttggttgg tggttggtga ttggtaacta gtaactagta actggtaact
     1861 ggtaactagt aactagtaac tagtaattg
//
LOCUS       DAOTON010000605         1888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47585_length_1888_cov_3.632842, whole genome shotgun sequence.
ACCESSION   DAOTON010000605 DAOTON010000000
VERSION     DAOTON010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47585_length_1888_cov_3.632842"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..>1888
                     /locus_tag="P5292_10920"
     CDS             89..>1888
                     /locus_tag="P5292_10920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HRS39683.1"
                     /translation="MQLPISLADRVNQSELIIEGEIISSQSKWNVERNMIFTVSQIRV
                     FKLFKGSLSGSVVTLLTEGGQDGLDLIEVGNEFMPVVGEQGVFMLKMNSKIPNLAIRF
                     QGPLYTCFMAHQSTVMYNLFDKTANSPFQQFNSIPYGLYPAIHQITGQYHTELITNTR
                     LNQALQQTQSGNQVNAVPTITSFTPTTITAGTRSLLTINGTNFGASRGAGFVEFRNAN
                     DGGASWVQAKASDYRTWTNTQITVWVPSSSLSNGPSAGTGQIRVTNNDPNIATSTGTL
                     TVTYSVINVEFDGKGQYTEHQNDNTTGGYTFQCEAATFAANAPAVAAFTRAMNTWTCS
                     TNMNWILGANTAVDTALADGINIVRFDKGPSLPAGVLGRATSRFSGCINAAAPSDTVW
                     TVTEVDVVYDDGAAGLSWNFSTANATFSQIDFETVTVHELGHAHQLQHIINSGAIMHY
                     SISNGQTSRVLSPTIDVPAGNFVMTRSTAAHGACKPSPMTSLSLAASVSIAASPGTSV
                     CSNTSVTFTATPVLPAGTATYTWKKNGVTVGTNSTTYTNASWVNGDQVQVVMSVSGGC
                     NQTVNSNTLTMTVSTAVTASVSIAASPGTTVCNG"
BASE COUNT          459 a          563 c          455 g          411 t
ORIGIN      
        1 ttcactttcc cggaaaagta ctttctccct actggcactg cttggatgcc tcttaatttc
       61 ggccaggttg aaagcaggag atgcttgtat gcagttaccc atttcacttg ccgaccgcgt
      121 caaccagtcc gagctgatca ttgaaggtga aatcatcagc agtcagtcga agtggaatgt
      181 tgaacgaaac atgatcttca cggtaagtca gattcgtgtt ttcaaactgt ttaaaggcag
      241 cttatcaggc tcggtagtta cactgcttac ggaaggagga caggatggac ttgatctcat
      301 tgaagtaggc aatgaattca tgccggtcgt cggtgagcaa ggcgtgttca tgctcaaaat
      361 gaactcgaag attccgaacc tggccattcg gtttcagggc ccactctata cctgcttcat
      421 ggcgcatcaa agcacggtca tgtacaacct ctttgataaa acagccaaca gtccgtttca
      481 acaattcaac agtattccat acggactata tccggccatt catcagatca ccgggcagta
      541 ccacacggaa ttgattacga atacccggct caaccaggcc ttacagcaaa cacagtcagg
      601 aaatcaggtg aacgcggttc cgactatcac ctcgttcact ccgacgacga tcaccgccgg
      661 cacccgcagc ctgcttacca tcaacggtac caatttcggt gcatcgcgtg gagcaggttt
      721 cgtcgagttt cgcaatgcca acgatggagg agccagctgg gtgcaggcca aagcaagtga
      781 ctaccgaacc tggaccaaca cacagatcac ggtctgggtt ccctccagtt cactttccaa
      841 tggcccttcc gccggaaccg gccagatccg ggtcacgaac aacgatccga atatcgccac
      901 cagtacagga acactgacgg tcacctattc tgtcatcaac gtggaattcg acggcaaggg
      961 tcagtacacg gaacaccaga acgacaatac cacaggtggc tacaccttcc aatgcgaagc
     1021 cgccaccttt gccgcgaatg cccctgctgt cgccgccttt acccgtgcca tgaacacctg
     1081 gacctgcagt accaacatga attggatcct gggagcaaac acagccgtgg acactgcact
     1141 tgccgatggc atcaatatcg ttcgctttga caaaggcccc agcctgcctg ccggtgtatt
     1201 gggcagagct actagccggt tttccggttg tatcaatgca gcggctccca gtgataccgt
     1261 ctggacagtc accgaagtcg atgtcgtgta cgatgatggt gctgcaggcc tttcctggaa
     1321 tttctcgaca gccaacgcca ccttctcgca aatcgacttt gaaacggtca ctgtacacga
     1381 attggggcac gcgcatcagt tgcagcacat catcaattcg ggcgcgatca tgcactactc
     1441 catctcgaat ggtcaaacct cccgggtgct ctcgcctacg atcgatgtcc cggccggaaa
     1501 tttcgtcatg acccggagta ccgcagccca cggagcctgc aaaccttctc ccatgacgag
     1561 cctctctctg gcggcctccg tttccattgc cgccagtccg ggaaccagcg tttgttcgaa
     1621 caccagtgtg acatttacgg ctacccccgt cctgcccgcc ggaaccgcca cttatacctg
     1681 gaaaaagaac ggcgttacgg ttggcacgaa cagcaccacc tacacgaatg catcctgggt
     1741 caacggcgat caggtacaag tggtgatgag cgtctcgggc ggttgcaatc aaaccgtcaa
     1801 ttcgaatacg ctcaccatga cggtcagcac ggctgttacc gcatccgtca gtatcgccgc
     1861 ttcacctggc acaaccgtct gcaacggg
//
LOCUS       DAOTON010000606         1887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47612_length_1887_cov_3.178493, whole genome shotgun sequence.
ACCESSION   DAOTON010000606 DAOTON010000000
VERSION     DAOTON010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47612_length_1887_cov_3.178493"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="P5292_10925"
     CDS             complement(<1..216)
                     /locus_tag="P5292_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRS39684.1"
                     /translation="MRQISDIIRHLRTDFSAFELNESDVEKNPLRQFAKWMEQALEAE
                     VNEPNAMTLATVNKKKQPDARIVLLRDV"
     gene            complement(220..696)
                     /gene="bcp"
                     /locus_tag="P5292_10930"
     CDS             complement(220..696)
                     /gene="bcp"
                     /locus_tag="P5292_10930"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRS39685.1"
                     /translation="MAKKRSGALQIGDKIPAFSLKNQDGKTVNHSDFLGRKLVLFFYP
                     KDNTETCTKEACNLRDHYKKLKKSGYEVVGVSMDSEKSHRRFIDKYDLPYDLLVDDQQ
                     ELVNAFKVYGQKVLFGRKYMGIIRTTFLIDEQGRIERIIESVDSARHAEQILTEQA"
     gene            863..1429
                     /locus_tag="P5292_10935"
     CDS             863..1429
                     /locus_tag="P5292_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRS39686.1"
                     /translation="MRKLIHLEPKVGRILVSEPFLMDSYFKRSVILLGEHGEEGTVGF
                     ILNKPTDLGLNDALEDFPPFEVPLYFGGPVQTDTIHFLHTIGAKLEGSKEILPGIWWG
                     GSLEMLKLMIDTHQVGKDDIRFFAGYSGWEPNQLNDELRSKTWLVSNCQKVFAFSDHP
                     EDLWGEVLRSMGSSYAVLANFPEDPSLN"
     gene            1735..1809
                     /locus_tag="P5292_10940"
     tRNA            1735..1809
                     /locus_tag="P5292_10940"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1770..1772,aa:Pro,seq:cgg)
BASE COUNT          434 a          458 c          499 g          496 t
ORIGIN      
        1 gacatcgcga agcaggacga tgcgtgcatc cggctgtttc ttcttgttga ctgtggcgag
       61 cgtcatggcg tttggttcgt tcacttccgc ttcgagtgcc tgctccatcc acttcgcaaa
      121 ttgccggagc gggttctttt caacatccga ttcgttcaac tcgaaggccg aaaagtcggt
      181 ccgcaaatgc cggatgatat cgctgatctg acgcatgggt tatgcttgtt cggtaaggat
      241 ttgttccgca tgacgggcgg aatctactga ttcgatgatc cgttcaatcc ggccctgctc
      301 atcgattaaa aaagtggtcc ggatgatgcc catgtacttt cgaccgaaga gcaccttctg
      361 accatacacc ttgaaggcat tcaccagttc ctgctgatcg tccaccaaca ggtcgtatgg
      421 aaggtcatac ttgtcgataa agcgacggtg cgacttttcc gagtccatac tcactcctac
      481 gacctcatat ccgctcttct tcaatttctt gtaatgatcc cgaagattac aggcttcctt
      541 cgtacaggtt tccgtattat ccttgggata aaagaacaag accagttttc gtccgaggaa
      601 atcggaatga tttactgttt ttccatcctg gtttttcaag ctaaaagcag ggatcttatc
      661 acctatttgc aacgctccgg atcgcttttt agccattttt taaggaattt cgcagcctgc
      721 tcaggatgta agttttacct atggaatgaa tgaactcctc ctgaaaacag gggtaaaact
      781 acgagaaaaa caaatgagag ccttttgggt ttcaactctt ccgggagtct ggcccagggc
      841 ttgactgacc tgatcaacga ttatgcgtaa gctgatacac ctcgaaccga aagtcggccg
      901 tatcctggtt tccgaaccct ttctgatgga ttcctacttc aagcgttccg tgattcttct
      961 gggagagcat ggcgaagagg ggaccgtagg atttatcctg aacaaaccga ccgatctggg
     1021 tctcaacgac gcgttggaag actttccgcc gttcgaagtt cccctgtatt tcggcggccc
     1081 ggttcagacg gatacgatcc acttcctcca caccatcgga gccaagctgg aaggaagcaa
     1141 agagatcctt ccgggtatct ggtggggcgg tagtttggag atgcttaaac tgatgatcga
     1201 tacgcatcag gttggcaagg acgacatccg atttttcgcc ggctattccg gttgggaacc
     1261 caaccagtta aacgatgaac tgcgcagtaa gacctggctg gtatccaatt gccaaaaggt
     1321 atttgccttc tccgatcatc cggaagacct ctggggagaa gtcctgcgtt ccatgggaag
     1381 ttcctatgcc gtactcgcga atttcccgga agacccttcg ctgaattgat gtagtgaagt
     1441 cgaaccgaga ctgagggacg tgagacgagg gacgagggac gagggacgtg agacgaggga
     1501 cgagggacgt gagacgaggg acgagggtcg agggagcagg gacgttgatc aaagactatt
     1561 cgtgccgttt taggttttag cttcttgtca cccagcaatt tcgatagctt acatcgttga
     1621 ttatcaactg actattcagc gtttcaaaaa gcatctgatc acattcctgg cttgtcattc
     1681 ttgctatttt attcgatttt cctacctttg ccgtccccaa aacagcgggg aattcggggt
     1741 gtagcgtagc ccggttagcg cgccacgttc gggacgtgga ggccggaggt tcgaatcctc
     1801 tcaccccgac agcatagtaa aagccttcca gtgatggggg gctttttttg ttagggacgt
     1861 ggggcgaggg acgaggtgcg gggtgtg
//
LOCUS       DAOTON010000607         1884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47698_length_1884_cov_4.102788, whole genome shotgun sequence.
ACCESSION   DAOTON010000607 DAOTON010000000
VERSION     DAOTON010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47698_length_1884_cov_4.102788"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1005
                     /locus_tag="P5292_10945"
     CDS             238..1005
                     /locus_tag="P5292_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009039491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRS39687.1"
                     /translation="MASILTYNVNGLRSALRKGWDQWIGTVAPDIVCLQEIKMMPDQL
                     PSGTLERIGYGAYWFPAEKKGYSGVAILSRKEPDRVVYGMDDPSDMEGRVIRADFGDW
                     SVLSVYVPSGTTGEERQSFKMDWLERFDGFLVKLRKKRPNLIVCGDFNICHKAIDIHD
                     PVRNAHSSGFLPEERAWMDRFTGKGWIDSFRHFSDSKDEYTWWSFRAGSRSKNKGWRL
                     DYQFVTDPLASSLKRCVHLQQAVHSDHCPVLLETSLK"
     gene            complement(1060..1566)
                     /locus_tag="P5292_10950"
     CDS             complement(1060..1566)
                     /locus_tag="P5292_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRS39688.1"
                     /translation="MKRILFLAFVLTAFVLNVHAQGPKLGHINSAELIQMMPQTKQAD
                     STLRRFAESLDGQMKTMTAEYQGKLQTYQSKVDSMPEAIRTTKQQELQDLGTRIQDFQ
                     QSAQESIQKKKEELYSPILQKAEDAIKEVAKEKAYSYIFDTSVGAVLYSTDSDDILPL
                     VKARLGIK"
     gene            complement(1591..>1884)
                     /locus_tag="P5292_10955"
     CDS             complement(1591..>1884)
                     /locus_tag="P5292_10955"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRS39689.1"
                     /translation="EGDLFKKKQELVKPIQDKIYNAIKKLATDQSIMAIFDKSGDLTI
                     LYANAKYDKSDDVLLALGYKPAKKDAAGSGKSGSGAPSTGGDKAPAMEQEPKK"
BASE COUNT          361 a          464 c          572 g          487 t
ORIGIN      
        1 ggctatgctc atctcccgca atgaactcaa ctgggcccat ccccggaacg ggaacagccc
       61 tgcggtcagg agaaaaagaa ccaggagtcg ttgcagccgc atgaacgttt aaaggtacag
      121 gttgccgggg gagcctgcgg acaccggaag ccttgttttt caagtatcat cccggaaaat
      181 tcgacaggta aggaaggctt tgttcatcgg ttcgtgcgca gcccgtatgt ttgtgccatg
      241 gccagtatct tgacatataa cgtaaacggt ttgcgctccg cgctccgcaa gggttgggac
      301 cagtggatcg gaaccgtagc gccggatatc gtgtgtttac aggagatcaa aatgatgccg
      361 gaccagcttc cgtccggtac actggaacgt atcggctatg gggcctattg gtttccggcg
      421 gagaagaaag gctattccgg agtagcgatc ctgagccgta aggagccgga tcgcgtcgtg
      481 tacggtatgg atgatccctc tgatatggaa ggtcgcgtta tccgggccga ttttggcgac
      541 tggtccgtac tcagcgtcta tgtgccatca ggtacaaccg gcgaggagcg gcagtcgttc
      601 aagatggact ggctggagcg ctttgacgga tttctggtta agctgcggaa gaagcgtccg
      661 aacctgatcg tttgtggcga tttcaatatc tgccataagg ccattgacat ccacgacccg
      721 gtccgcaacg cccattcttc cggtttcctg cccgaggaac gggcctggat ggaccggttc
      781 accgggaaag gttggatcga ttccttccgg catttttcag atagtaagga tgaatatacc
      841 tggtggagtt ttcgtgcggg ctcgcggtca aagaacaaag gctggcggct cgactaccag
      901 ttcgttaccg atccgctggc ctccagtctg aagcgctgcg tccacctgca gcaggctgtg
      961 cacagcgatc actgcccggt gttgttggag acatcgctga agtgaaataa aaaagccatc
     1021 cgtttcaggc ggatggcttt tttgtttgaa ggctgcggat tacttgatac ccagtctggc
     1081 tttcaccagc ggtaagatgt cgtcgctatc ggtggagtag aggacagcgc ctacgctggt
     1141 gtcgaaaata tagctgtagg ctttctcttt agcgacttcc ttgatggcat cttcagcttt
     1201 ctgcaggatc gggctgtaga gctcttcttt cttcttctgg atggattcct gcgcgctttg
     1261 ctggaaatcc tgaatgcggg ttccgagatc ctggagttcc tgttgcttcg tggtgcggat
     1321 ggcttccggc atggagtcca ctttcgactg gtacgtctgg agcttgccct ggtactcggc
     1381 ggtcatggtt ttcatctggc cgtcgagcga ttctgcgaaa cgacgcaggg tactgtcggc
     1441 ctgcttggtc tgcggcatca tctggatgag ttcggcggaa ttgatgtggc cgagtttagg
     1501 accctgggcg tggacattca gcacgaaggc agtcagcacg aaggccagga agagaatacg
     1561 tttcattgtt caggtgtggt ttagaatgga ttatttcttg ggttcttgtt ccatggccgg
     1621 cgccttgtcg ccgccggtgg aaggagcgcc gctgcccgat ttaccgctgc cggccgcgtc
     1681 tttcttggcg ggcttataac cgagcgcgag caggacatcg tcgctcttgt cgtatttggc
     1741 gttggcgtag aggatggtca ggtcgcccga tttgtcgaag atcgccatga tgctctggtc
     1801 cgttgccagt ttcttgatgg cgttgtagat cttatcctgg atgggtttca cgagttcctg
     1861 cttcttcttg aagagatcgc cttc
//
LOCUS       DAOTON010000608         1883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47739_length_1883_cov_3.102298, whole genome shotgun sequence.
ACCESSION   DAOTON010000608 DAOTON010000000
VERSION     DAOTON010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47739_length_1883_cov_3.102298"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..532
                     /gene="gldC"
                     /locus_tag="P5292_10960"
     CDS             191..532
                     /gene="gldC"
                     /locus_tag="P5292_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HRS39690.1"
                     /translation="MKKSAIHFDITVDNDFIPHTIEWQATDSGVEGKKNCHATLLSLW
                     DPAEKATLRIDLWTKDMLVDDMKRFFYENFATMADTYMRATNDAELSKMIRDFAKEFG
                     DKAGLFAKPKS"
     gene            complement(533..1156)
                     /gene="yihA"
                     /locus_tag="P5292_10965"
     CDS             complement(533..1156)
                     /gene="yihA"
                     /locus_tag="P5292_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRS39691.1"
                     /translation="MAGYTIRDAEFIVSNTSVAKCPAADRPEFAFIGRSNVGKSSLIN
                     MLTVRKGLAKTSATPGKTQLINHFLVNNAWYLVDLPGYGYARTSQSTKAGWDKMIRDY
                     LLKRENLMTVFVLVDVRHEPQKIDVEFINWLGEQGVPFCIVFTKADKLSKQQVIRSVE
                     SYKRTLRTTWEELPMLFITSAQMRNGREEILDYILSVAADFKPSRKP"
     gene            complement(1156..>1883)
                     /locus_tag="P5292_10970"
     CDS             complement(1156..>1883)
                     /locus_tag="P5292_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39692.1"
                     /translation="KNKDHRFYAGFSLAFGLPQAEFRDNLNSIGIGIDGMFAWQPSHR
                     IPVALGFDVGYMAYAMHTQRQTLYAEVKSGNTVISTLEIPLRVETASSIVDPQVLLRF
                     IAPTKKVVPFVDAIFGFTYFRTGTSIYDESSRRQLSSAEDGLITRDTNLDDWTMNYGC
                     ALGLQYELKPTLAIHVRCAYIFGGEAQYYSGKQAESWDINLTTTPTDPNNISANELDV
                     NATPYESRTDMINIKAGVTLKFD"
BASE COUNT          429 a          461 c          544 g          449 t
ORIGIN      
        1 gtttccctaa ggagtcgccc ggtaacatag cgttgtatat cggatggcgc atcgtcgaat
       61 cttacatgga gcgtcatcct gacgtgagca tcagacaact gctggaagaa aaagacctca
      121 accgggtata ccaggagtcg ggctataagc cgcataaacc ctgaaagggc aaccctgcaa
      181 tcaaacaaac atgaaaaaat ccgcgataca cttcgatatt accgttgata acgacttcat
      241 cccgcatacc attgaatggc aggcaaccga cagcggcgta gaaggaaaaa aaaattgtca
      301 cgccactttg ctgtcgcttt gggaccctgc cgagaaagcc acactacgca ttgacctttg
      361 gacgaaggac atgctggtgg atgacatgaa gcggttcttt tatgaaaact ttgcaacgat
      421 ggccgacacc tatatgcgtg ccacgaacga tgccgagttg tcgaagatga tccgtgattt
      481 cgcgaaggag ttcggagata aggccggact ttttgcaaag cccaaatcct gatcagggtt
      541 tgcgtgacgg cttgaaatcc gcggcgacgg agaggatgta atccaggatc tcttcccgac
      601 cgttccgcat ttgagcggag gtgataaata gcatcgggag ttcttcccag gttgtccgca
      661 gcgtcctttt gtaggattca acggaacgga tgacctgttg tttggagagt ttgtcagctt
      721 tggtaaagac aatgcagaac ggtactccct gttcgcccag ccagttgatg aattccacat
      781 cgatcttctg aggttcgtgc cggacgtcga ccaagacgaa gaccgtcatc aggttttcgc
      841 gtttaagcag gtaatcgcgg atcatcttat cccatcccgc tttcgtggat tgcgatgtgc
      901 gcgcgtaacc gtaccccggg aggtcgacca ggtaccaggc gttgttgacc aggaagtggt
      961 tgatgagctg cgtttttccc ggtgtagcag aggtcttggc caggccttta cgcaccgtca
     1021 gcatgttgat cagggaagat ttaccaacat tggaacggcc aatgaatgcg aactcgggcc
     1081 ggtccgcagc cgggcatttc gcaacggaag tgttgcttac aataaattcc gcatcgcgga
     1141 tcgtatagcc ggccatcagt cgaatttcaa ggttacgccc gccttgatgt tgatcatgtc
     1201 ggttcgggac tcgtaaggtg ttgcattcac atcaagctcg ttcgcggaga tgttattggg
     1261 atcggtcggc gtggtggtga ggttgatgtc ccagctttcg gcttgtttgc cggagtagta
     1321 ttgggcctca ccgccgaaga tataagcgca gcggacgtgg atggcgagtg tgggtttcag
     1381 ttcgtattgc aatcccaatg cgcagccgta gttcatggtc cagtcgtcga ggttggtgtc
     1441 gcgcgtgatc agcccgtcct ccgcactcga caactgccga cgggatgatt cgtcgtagat
     1501 gctggtcccg gtccggaagt aggtgaagcc gaagatcgcg tcgacgaacg gaaccacttt
     1561 tttcgtggga gcgatgaaac gcaggagcac ttgcgggtcg acgatcgaac tggccgtttc
     1621 aacccggagg ggtatttcca aggtgctgat gaccgtgttt cccgatttca cttcggcata
     1681 cagtgtttgc cgttgggtat gcatggcgta ggccatgtag cccacatcga acccgagtgc
     1741 cactggaata cggtggctgg gctgccaggc gaacatgccg tcgataccga taccgatgga
     1801 attcaggttg tcccggaatt cagcttgtgg gagtccgaac gcaaggctga agccggcata
     1861 gaaccggtgg tccttgtttt ttt
//
LOCUS       DAOTON010000609         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_47971_length_1877_cov_2.302415, whole genome shotgun sequence.
ACCESSION   DAOTON010000609 DAOTON010000000
VERSION     DAOTON010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47971_length_1877_cov_2.302415"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="P5292_10975"
     CDS             complement(<1..1126)
                     /locus_tag="P5292_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS39693.1"
                     /translation="MGNATPNIGWGPMEIHGTGSCYCDSVVSNCSTPCPDGSYPKELV
                     IQRIYHKDGGSMTYFDRPAGTMSYHPTHGHVHVDNWADFTLRRATPDPDSRNWPIIGT
                     GNKQSFCLVNLGDCDANYGYCVDDQGVTLNKSDIPNSDLGSVSGCSRDQGIYVGNLDI
                     YSSGLNGMGIVLPSTTCNGDYYIVSITDPMNNFLETNDQNNWVAVPITLFQQSAGSFP
                     ISGYTYTVLANEVDFTASALGTDSLVWRFGDGSSAVTTTSPGIQHTFPGVGSYIVTLY
                     AYNTCGPTVTIDTVQILSTTGIEQINDLVNFTASPNPSSDVFRVNYTLINPSSVQLDL
                     LDMTGRRIASLYSGNQPAGKFLQTLDIRELGLASGIYNLRL"
     gene            complement(1056..1775)
                     /locus_tag="P5292_10980"
     CDS             complement(1056..1775)
                     /locus_tag="P5292_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HRS39694.1"
                     /translation="MKKLLLLLAFLPLGSFAQSFTNTTATPITGGDVLIPIQVNGIAN
                     QINGTFGLASVCLDISHEFVEDLQLRLQSPDGKIIDLSIGHGGNGPGYRNTCFMMNGT
                     GGRIQYAPAPFTGTYLPEMSLNVFNDGSDPNGTWLLRIRDIFPTADTGTFHFVGLLHD
                     QSAGRSYCQRWSVQYLRCHRLRLSGWGFYRLRPAPGHDLLGALYPTRPPGIRRKHYDG
                     QRNAQHRLGTHGNTWYRFLLL"
BASE COUNT          454 a          467 c          526 g          430 t
ORIGIN      
        1 gaaggcgcaa gttgtaaatt ccggaagcga gaccgagttc acgaatgtcc agtgtctgaa
       61 ggaattttcc tgcaggctga ttaccggaat aaagcgaggc gatccgacga ccggtcatgt
      121 ccagaagatc aagctgaacg gacgaaggat tgatcagggt atagttcacc cggaatacat
      181 cgctcgaagg attcggactg gctgtgaagt tgaccaggtc gttgatctgc tcgataccgg
      241 tagtcgacag gatctgtacg gtgtcgatcg tcaccgtcgg tccgcaggta ttgtacgcat
      301 acaaggtcac gatgtagctg cctacacccg ggaatgtgtg ttgaatgccg ggactcgtgg
      361 tggtaacggc agaagaaccg tcaccgaatc gccacaccag agaatcagta ccgagagcag
      421 aagcggtaaa gtcgacttcg ttcgccaata ccgtatacgt gtaaccggag atggggaagc
      481 tgcctgcact ttgctggaaa agtgtgatcg gaactgccac ccagttgttc tggtcgtttg
      541 tctcaaggaa attgttcatc ggatcggtga ttgatacgat gtaatagtca ccattacagg
      601 tggtcgacgg cagtacgatg cccatcccgt tcaatcctga gctgtaaata tccaggttac
      661 cgacatagat accctgatcg cgtgaacaac ccgatacact gccgaggtcc gagttcggga
      721 tatccgattt gttgagtgtc acgccctggt catccacgca gtagccgtag ttggcatcac
      781 aatcaccaag gttcaccaga cagaaactct gtttgttacc cgtgccgatg ataggccagt
      841 ttcgggaatc aggatccggt gtagcgcgac gaagcgtaaa gtcggcccag ttgtcgacgt
      901 gcacgtgccc gtgggtcggg tgatagctca tcgtgccggc cggacggtcg aagtaggtca
      961 tgctgccgcc gtctttgtgg tagatgcgct gaattaccaa ttcttttgga tacgatccgt
     1021 caggacaggg agtgctgcaa ttggaaacga ccgaatcaca atagcaggaa cctgtaccat
     1081 gtatttccat gggtccccaa ccgatgttgg gcgttgcgtt gcccatcgta atgtttccgg
     1141 cgtattcctg gtggtcttgt tggatacaaa gcaccgagga ggtcatgtcc gggagcaggt
     1201 cgcaatcggt agaaacccca tccggacagg cgcagccggt ggcatcggag gtattgcacg
     1261 gaccaccgtt ggcagtagga tctgccggcg gattgatcat gaagaagacc gacgaaatga
     1321 aaggtgcccg tatcggctgt cgggaagatg tcacggatcc tcaacaacca ggtaccgttg
     1381 ggatccgaac cgtcgttgaa tacgttcaac gacatctccg gcaggtaagt gccggtgaag
     1441 ggtgccggtg catactggat acgaccaccg gttccattca tcatgaaaca ggtgttccgg
     1501 tatcccggtc cgttcccgcc atgaccaatc gaaaggtcga tgatcttgcc atccggtgat
     1561 tgcaggcgta actgcaggtc ttccacaaac tcgtggctga tatccaggca taccgaggca
     1621 agaccaaacg tgccattgat ctggttggcg atgccgttga cctgaatcgg aatcaacaca
     1681 tcaccacccg taatcggcgt agcggttgta ttcgtaaaac tttgggcgaa tgatcccagc
     1741 ggcaagaaag ccagcaggag taacagtttt ttcatgtggg tgcgtttact tgggtaaatt
     1801 taagtgtttt atcctaacat acaaccactt aggcaatcct cgatttgtcc ccaatcttat
     1861 caaccggccc catcctg
//
LOCUS       DAOTON010000610         1872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_48155_length_1872_cov_2.094111, whole genome shotgun sequence.
ACCESSION   DAOTON010000610 DAOTON010000000
VERSION     DAOTON010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48155_length_1872_cov_2.094111"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            594..>1872
                     /locus_tag="P5292_10985"
     CDS             594..>1872
                     /locus_tag="P5292_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39695.1"
                     /translation="MENKVYFVPGFFRWLRALCLTGLFITGLSLTSTAQVTTNSGSGL
                     APSYSDLATAITALNGATISSPVVITLTANETAPAGGYSITAQGSSSNSITIDGGGYI
                     ITANGTLTAGALNDALIKLVGADFVTIQNFVLQENPANTTTTAASNNMTEWGVALLYA
                     STTNGASSNTIQNNTISLSRLYQNSFGIYANATHSASAPSTSATATGAAGAHDSLRVY
                     TNNISNVNNGIVVVGPTAAADQADYVDIGGSSAGTGNTISNYGNTGTFSSYANVSGTV
                     YGILVRNTRNINVQYNSITSSNTTGALTSSGTLRGIYVPSFSVAPTGTNSQVISNNTI
                     SVTTGVATGTLHGISVEATTGNATTSLTINTNTFQNCGHNVASPSGTTIFIISAMANL
                     NTTINNNVFSNLSTPSTGSVTFISNSITIPANGT"
BASE COUNT          469 a          554 c          397 g          452 t
ORIGIN      
        1 ttattcaacc aaccggaaac cggaaactgg aaacccgaaa cccgaaaccc gtaacttttt
       61 ctcgtccctc gtccctcgtc cctcgtccct cctcccggca cgatccttgc gacccctaac
      121 aacaaccgcg ccgtttatct tttttttcat ttaacccgga actcggaacc cgaaacccga
      181 aacttagaac cctggttccg ttcaccacgc ttttcccgcc attggccaaa atggatagga
      241 tttaattaca agccggtctt gtaatcgtcc cgcttatttc ttaatattcg gaattgaaaa
      301 tgcgctgaaa agcaagcctg ctgcgggttt cgttaacaca tgttgataaa tcaggcttgg
      361 aaaagtgtca gaaaaaaacc atctttgatt cgtccgtagt atcaacctgc gggcgaatgg
      421 ggaacacaga ataaactcaa gctgcgtgaa acacttctac ccgacgtgta atcggtcttg
      481 tcatcttctg ctcaacgcga atgcagcgtc gtgccttggc accgctgcat ttttcattat
      541 acactcccga taattcatca actcaaacca ataattaact aacacacacc aacatggaaa
      601 acaaagtcta ctttgtccca ggattcttcc gttggttgcg tgcgctctgt ctgacaggac
      661 tgttcatcac aggcttgtcg ctgacatcta cggcgcaggt aacaaccaac tcgggatccg
      721 gcctggctcc gtcgtattcg gatctggcca cggcgattac ggcgttgaac ggcgcgacga
      781 tctcgtcacc tgtcgtcatt acactgaccg ccaacgaaac ggctcctgcc ggcggttatt
      841 ccatcactgc ccagggttcg tcttcgaact cgatcaccat tgacggtggc gggtacatca
      901 tcacggcgaa cggaacgctg acggccggtg cgctcaacga cgcgctcatc aagctcgttg
      961 gtgccgactt cgtcacgatc cagaacttcg tgctgcagga gaacccggcc aacaccacca
     1021 ccaccgccgc ctcgaacaac atgacggaat ggggcgtggc cctgttgtat gcgtctacca
     1081 caaatggagc cagcagcaac accattcaga ataacaccat ttcccttagc cggctttacc
     1141 agaattcttt cggtatctac gctaacgcga cccattcggc ttccgctccg tccacttcgg
     1201 ctacggcgac tggtgcagca ggtgcgcacg actccctccg ggtttatact aacaatatca
     1261 gcaacgtcaa caatggaatt gttgtagttg gaccgaccgc tgctgctgat caggcggact
     1321 atgtcgacat cggcggatct tccgccggaa ccggtaacac tatttccaat tacggaaata
     1381 ccggtacttt ttccagttat gccaacgttt cgggaacagt atatggtatc ctggttcgga
     1441 atacccggaa cataaacgtt caatataact ccattacgag ttccaatacg acgggtgcct
     1501 tgacctcctc aggtactttg cgtggtatct atgttccttc tttcagtgtt gctccaaccg
     1561 gaacaaacag tcaggtaatc agcaataaca cgatttcggt aacaactggc gttgctaccg
     1621 ggacattgca tggtatttcc gttgaagcaa caaccggtaa tgccaccaca agcctgacca
     1681 ttaacaccaa tactttccag aactgcggcc acaatgtggc ttctccttcg ggcacgacca
     1741 tctttatcat cagtgcgatg gctaacctga acaccactat aaataataac gtgttcagca
     1801 acctgtcaac cccctccacc ggcagtgtaa cctttatctc aaacagcatt acaatcccgg
     1861 ccaatggtac gc
//
LOCUS       DAOTON010000611         1871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_48194_length_1871_cov_2.533040, whole genome shotgun sequence.
ACCESSION   DAOTON010000611 DAOTON010000000
VERSION     DAOTON010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48194_length_1871_cov_2.533040"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="P5292_10990"
     CDS             complement(<1..347)
                     /locus_tag="P5292_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRS39696.1"
                     /translation="MKKLTHLIPMSLISLSLVLALPGCQSGAQEEGATNANQSTESTP
                     VEGGQSTVEDDVSQPNVVKVAVGSKDHTTLVEAVEAAELVDALSNNGPFTVFAPTNDA
                     FAALPAGTVEGLL"
     gene            470..1009
                     /locus_tag="P5292_10995"
     CDS             470..1009
                     /locus_tag="P5292_10995"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRS39697.1"
                     /translation="MIRLRTFDLGDADAIASLANNRKIADNMRDMFPHPYHREDAVRF
                     IQRVNQQSPSTVFAIELDGKPIGSIGYFPASDINRMNAEVGYWLGEPFWGKGIITEAI
                     GKLVDHAFTHSNLSRLFAIPFPHNLASAKALARNGFELEARLKGTLIKHDRVMDELIY
                     SIRREEWAKRFPEKAKART"
     gene            complement(1041..>1871)
                     /locus_tag="P5292_11000"
     CDS             complement(1041..>1871)
                     /locus_tag="P5292_11000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HRS39698.1"
                     /translation="KKKAEEKLGRITSKVCYPDNWKDYSSMRISRDSYLGNVMNARIW
                     QYQYIVGKLYKPVDRNEWEMTPQTYNAYYNPSNNEIVLPAAQFLIPGLPDSLADDAII
                     YGYAAASTIGHELTHGFDDQGRQFDAAGNLKNWWTAEDSSRFTQRAKLIIDQFSNYTV
                     LDSLHVNGEATLGENIADLGGVVIGLEAFKKTEQYKKGEKIDGLTPMQRYFLGYTLGW
                     LGHSRDASLANQVMTDVHAPNFLRVNGPFSNVPEFYEAFGIKQGDPMWRPDSLRASIW
                     "
BASE COUNT          411 a          490 c          502 g          468 t
ORIGIN      
        1 tttaggagac cttccaccgt gccggccggc aaggctgcaa atgcatcatt ggtaggtgca
       61 aataccgtga acgggccatt gttcgagagc gcgtcgacca gttcagcggc ctctaccgct
      121 tcgaccaatg tggtatggtc ctttgaccct accgccactt tcacgacgtt aggctgggag
      181 acatcatctt ctactgtgga ttggccacct tccacgggtg tggactcggt tgactgattg
      241 gcgttggttg ctccttcttc ctgagcgccg gactggcatc ccggtagtgc caaaaccagg
      301 cttaaggaaa tgaggctcat cgggatcaaa tgtgtcagtt ttttcatggg tgcgggtttg
      361 attaacttgc cagtgaaatt acaaccgtcg taagcagcgg gtgactgatt gccctcaggg
      421 cacgtactgc tctttatcat tccctcgcct acgaaaacac accattccga tgatccgcct
      481 acgaacattc gatctgggag acgccgatgc gatcgccagt ctggccaaca accggaagat
      541 cgccgataac atgcgcgaca tgtttcctca cccgtatcac cgggaagatg cggttcggtt
      601 cattcagcgc gtcaatcagc agtcgccttc taccgtcttc gccatcgagc tggacgggaa
      661 gccgatcggc tcgatcggtt attttccggc atccgacatc aaccgcatga acgcagaggt
      721 cggttattgg ttgggggaac cattttgggg caagggaatc atcacagaag ccatcggcaa
      781 gctggtcgac catgccttta cgcattcgaa tctgagtcgc ctctttgcca ttccgtttcc
      841 gcacaacctg gcttccgcca aagcgctggc gcgaaacggt ttcgaactgg aggctaggtt
      901 gaagggtacg ctcatcaaac atgaccgggt gatggatgag ctgatctatt ccattcgtcg
      961 cgaggagtgg gccaaacgat ttccggaaaa agcaaaggcc cggacatgac cgggcctttc
     1021 gctttgaagt agctacagtg ttaccagatg cttgcacgaa gtgagtccgg gcgccacatc
     1081 ggatcgcctt gcttgatgcc gaaggcctcg tagaattcag gaacgttgct gaacgggccg
     1141 tttacccgga ggaagttcgg agcatgaaca tcggtcatga cctgattagc aagacttgca
     1201 tcacgggaat gtcccaacca tcctaaggtg tagcccagga agtaacgctg catgggcgtc
     1261 aggccatcga tcttctcgcc tttcttgtat tgctccgttt tcttgaacgc ttccaaaccg
     1321 atcactacgc cgccaagatc tgcgatattt tcgccgagtg tagcctcacc attcacatgc
     1381 aggctgtcca ggacggtata gttgctgaat tgatcgatga tgagtttcgc acgttgggtg
     1441 aagcgggagg agtcttccgc tgtccaccag tttttcaggt tgccagccgc atcgaattgc
     1501 cgaccctggt cgtcgaatcc atgggtcaat tcatgtccga tagtcgaagc ggcggcatat
     1561 ccataaatga tcgcgtcatc ggccagggaa tccggtaaac cgggaatgag aaactgtgca
     1621 gcaggcagga cgatctcgtt gttgctgggg ttgtagtaag cattatacgt ctgcggagtc
     1681 atctcccatt cgttccggtc aaccggtttg tagagtttcc cgacgatgta ttgatattgc
     1741 cagattcggg cgttcatgac atttccgaga taagaatcac gggagatgcg catggaactg
     1801 tagtctttcc agttatcagg ataacagact ttactggtta tacgtcccaa tttttcttca
     1861 gccttctttt t
//
LOCUS       DAOTON010000612         1870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_48221_length_1870_cov_3.430854, whole genome shotgun sequence.
ACCESSION   DAOTON010000612 DAOTON010000000
VERSION     DAOTON010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48221_length_1870_cov_3.430854"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..538)
                     /locus_tag="P5292_11005"
     CDS             complement(221..538)
                     /locus_tag="P5292_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39699.1"
                     /translation="MKRFGRSVLSVFFLLVFLYPLAEKTLHELSHAGDVHCNTVSQYH
                     LHQEEHHCEVCDFTLTGTAEFSAIPLQCLCVVASTIGIPPLTTSVLVEPPYFFQLRAP
                     PLA"
     gene            complement(855..1442)
                     /locus_tag="P5292_11010"
     CDS             complement(855..1442)
                     /locus_tag="P5292_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HRS39700.1"
                     /translation="MTSHKTLSLLGIAAALAGAIACSSPAPEGDKKTTETVDENAKVE
                     RGRYLVGIMGCDDCHSPKVMGPMGPEPDTSRRLSGHPADMPLGPSDTNVFANGWVLMS
                     MQLTTTHGPWGKTYAANLTSDETGIGNWSYEQFKKAMTEGKYKGMDNTRPLMPPMPWP
                     NFRNLHEDDLQAIFAFLKSTKPVKNRVPDYAPPGS"
BASE COUNT          430 a          483 c          571 g          386 t
ORIGIN      
        1 agccgcgtgc ggtcgtggtc gtcgagcacg ctgcccgaga gcgagccggt acaggcttgc
       61 gcgaacaggg acgcggggcg catcagcaga aaactgccaa tgagcaaaag gtgcagtcgt
      121 ttcacgattg caggttaagg cgatgcacgt tcgctatgaa gtgaacggat cggtggtgat
      181 gaaagaagca gggaaggatc cgccgcaggc ggaaaagcga tcaggccagg ggaggcgccc
      241 gaagctggaa aaagtacggt ggttcaacga ggaccgaagt cgtcagcgga ggaatgccga
      301 tcgtcgacgc gaccacacac aggcactgca acggaatcgc ggaaaactcg gcggtgcccg
      361 tcagcgtgaa atcacagact tcgcagtgat gttcctcctg gtgtaagtga tactgggaaa
      421 ccgtgttaca atgaacatcg ccggcatgcg aaagctcatg cagggtcttc tccgccagcg
      481 ggtacaggaa taccagcaga aaaaaaacac tcagaaccga ccgaccgaag cgcttcatgg
      541 gaaggttgca aagatacaaa gcggcggtta gtaaagtatg aagggaattt ggagtaggaa
      601 acggtttgta tttggttgca agttgaaggt tgcaggttgc aggttgaaag aaggaagttg
      661 aaagatgaaa gttggaaatt gaatgttttt cggctttcgt ttaaaatcag gcggtttcgc
      721 aaattgagcc gcgtcccgtc gccagtcagc ggttttctct ccagaatagt cgccccacta
      781 ccagtcaaac gatcagcctg caaccttcaa ctttcaactt tcaaccttca cgttacaacg
      841 tttaaacgaa aaaatcagct tcccggcggc gcataatccg gcacgcggtt cttcaccggc
      901 ttggtcgact tcaggaaggc gaagatcgcc tgcaggtcgt cctcgtgcag gttgcggaag
      961 ttcggccagg gcatcggtgg catgagcggt cgggtgttgt ccatgccttt gtatttgcct
     1021 tcggtcatcg ccttcttgaa ctgctcgtag ctccagttgc cgataccggt ttcgtcggaa
     1081 gtcaggttcg cggcgtaggt cttaccccag gggccgtgcg tagtcgtgag ttgcatggac
     1141 atcagcaccc agccgttggc gaacacgttc gtatcggagg ggccaagcgg catatcggcc
     1201 gggtggcccg acaggcggcg cgaagtgtcg ggttcgggac ccatcgggcc catcactttg
     1261 gggctgtggc agtcgtcgca gcccatgatg ccgacgaggt agcgtccgcg ttccaccttg
     1321 gcgttctcgt cgacggtttc cgtggttttc ttgtcgccct cgggagcagg cgaggagcag
     1381 gcgatcgcgc cggccagcgc ggcggcgatg cccagcaggc tcagggtttt gtgggaggtc
     1441 ataaagggga tttggtttgc gggcgaatgt aaaatatttc attcacctcg cccatacaac
     1501 aaaagactca actgtcaaaa acgtccgatg aaacgtttca gtgacaggat gaacatcaag
     1561 aggagcggag ccgcggatcc cgctcgccac ccgtcccgtg ttcgcccggc atggataccg
     1621 tgtgcatccc atcagcacca gacggtgccg gactcacccg gggctaacct ggagcgtcgg
     1681 tgtttcaact caacaagtcg gggagtagtc acacctacat gcttcaagca gattttctgc
     1741 gtgtcatgct gcgcgttgcg ctgtagaata ttagcacccg cgcacatccg caacatccgc
     1801 gagatccgcg tgctgtcaat ttgtgaccac tttcggtagg cataggatta gtagcacaga
     1861 gtaacccgga
//
LOCUS       DAOTON010000613         1870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_48232_length_1870_cov_2.665014, whole genome shotgun sequence.
ACCESSION   DAOTON010000613 DAOTON010000000
VERSION     DAOTON010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48232_length_1870_cov_2.665014"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1870
                     /locus_tag="P5292_11015"
     CDS             <1..>1870
                     /locus_tag="P5292_11015"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39701.1"
                     /translation="YASTAGNRIIELRNGAGNLLQSKTINLPVGESRVYLNFDVPQEN
                     DLTLGLGAGSLGLFRNNSGVSYPYDVSRTLEIYGSNSGPNFYYYFYDWYVFVPYQNCA
                     SNRVTASAQIGSGASNAFDRSRCGNGSVTLSANSPDALTWWDAASGGTQVGSGSSYST
                     PGLSSTTSYYLQAGSCPSRIEVQAIINAQSSAPTASDVTNCGPGAVTLTASSPDPISW
                     YNLASNGFLLGSGSSYITPYLTETDTFYAVAGTACPSSAVAVRAIINSAAAPTGTDAS
                     ACGPASVTLSATSTDPIEWYDAAIGGNLVGTGPTLVTPVLGATTTYYAQAVGGCASVR
                     TPVTANITVVSPPVGTSASRCGSGTLVISAASIDPVTWSDAASGGTQIGSGINFTTPV
                     LTSTTTYYAEAANNGCSSTRTAVDAEIIVTPPPTVTSGSVCISGSVILTASSTDSIAW
                     YDAPTGGNLLGTGSSFTTPVINSTTSYYVEAGLSCPSVRVPVDAIVAGPSSTPTVTGA
                     SNCGSGSVTLTASSPDPISWYDAPSGGTLLGTGASFTTPVISTTTTFYAIAGITGCES
                     APASVDAEILPLAADPVTTGASNCGPGSLTLNATAADPMSWYDAPSNGTLIATGTS"
BASE COUNT          358 a          652 c          500 g          360 t
ORIGIN      
        1 tgtacgcgag cactgccggc aaccggatca tcgaattgcg caacggagca ggcaacctcc
       61 tgcaatccaa aaccatcaac ctgccggtgg gcgaaagtcg ggtttacctg aacttcgacg
      121 tgccgcagga aaacgatctc acgctcgggc ttggtgccgg tagcctcggg ctcttccgga
      181 ataactccgg tgtttcgtat ccatacgatg tttcccgtac gctggagatc tacggctcca
      241 acagcggccc gaacttctat tactacttct acgattggta tgtcttcgtt ccgtatcaga
      301 actgcgcttc caaccgtgtt accgccagcg cgcagatcgg cagcggcgct tcgaacgcgt
      361 tcgaccgctc ccgttgcggc aacggcagcg taaccctcag cgccaactct cctgacgcac
      421 tcacctggtg ggatgcggcc agcggcggca cacaggtagg cagcggttcc tcctactcca
      481 cacccggcct cagcagcacc accagttact acctgcaggc cggctcctgc cccagccgta
      541 tcgaagtaca agcgatcatc aacgcgcaat cctccgcgcc gaccgcttcc gatgtgacga
      601 actgcggtcc cggcgcggtg accctgaccg catcctctcc tgacccgatc tcctggtaca
      661 acctcgccag caacggcttc ctcctgggca gcggtagcag ttatataacg ccttacctca
      721 ccgaaaccga tacgttctac gcggtggccg gcacggcttg tccctcttcc gcggtcgcgg
      781 tccgcgcgat catcaactcc gccgccgcgc ctaccggcac ggatgcctcc gcttgcggac
      841 ctgcttccgt aacactctcc gccacttcca ccgatccgat cgaatggtat gacgcggcca
      901 tcggcggtaa cctcgtcggc accggcccga cactcgttac gccggtactc ggcgcgacga
      961 ccacctatta cgcgcaagcg gttggaggtt gtgcctccgt tcgcacgccc gtgacagcca
     1021 acatcacggt ggtcagtcct ccggtaggta ccagtgcatc gcgttgcggc agtggcacgc
     1081 tcgtgatctc cgctgcttcg atcgatcccg tcacctggtc ggatgccgca agcggcggaa
     1141 cgcagatcgg cagcggcatc aacttcacca ccccggtact gacatcgact acgacctact
     1201 acgcggaagc cgccaacaat ggctgctcca gtacgcgcac cgcggtcgat gcggagatca
     1261 tcgttactcc tcctccaacg gtgacgagcg gaagcgtctg tatttccgga tctgtcatcc
     1321 tcacggccag ctccaccgat tccatcgcct ggtacgacgc gccgacgggt ggcaacctgc
     1381 tcggtaccgg tagcagcttc acaacacctg tgatcaacag cacaaccagc tactacgtgg
     1441 aggcaggctt gagttgtccg agcgtacggg ttcccgtgga tgcaatcgtc gctggcccgt
     1501 ccagtacacc gacggtaaca ggtgcttcga attgcggcag tggtagtgtt accctgacag
     1561 ccagctcccc tgatccgatc tcctggtacg acgcgccaag cggcggcaca ttgctcggca
     1621 ccggcgcttc attcaccact cctgtcatct cgacaacaac gaccttctat gccatcgccg
     1681 gtatcacggg atgtgaaagc gcaccggctt ctgttgatgc ggagatcctg ccactggccg
     1741 ctgatcccgt tacgaccgga gcttcgaact gcggcccggg ctcgctgaca ttaaacgcaa
     1801 cagcggcaga tccgatgagc tggtacgacg ccccgagcaa cggtacgctc attgccaccg
     1861 gcaccagcta
//
LOCUS       DAOTON010000614         1869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_48271_length_1869_cov_2.730430, whole genome shotgun sequence.
ACCESSION   DAOTON010000614 DAOTON010000000
VERSION     DAOTON010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48271_length_1869_cov_2.730430"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..1645)
                     /gene="fumC"
                     /locus_tag="P5292_11020"
     CDS             complement(242..1645)
                     /gene="fumC"
                     /locus_tag="P5292_11020"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRS39702.1"
                     /translation="MQSYRKEKDTMGVVEVPANVYWGAQTQRSIDNFRIAQDINRMPK
                     EIVRAFAYLKKAAALANLDAGVLPKEKCDLIGQVCDEILAGKLDDQFPLVVWQTGSGT
                     QSNMNVNEVVAYRAHVISGGQLTDEKKAVHPNDDVNKSQSSNDTFPTAMHIAAYKILM
                     DVTIPGIEKLRDTLAAKAKEYKNIVKIGRTHLMDATPLTLGQEISGYVSQLEHGLRAI
                     RHTLPHLAELALGGTAVGTGINTPKGYDVNVAKHIAKLTGLPFVTAENKFEALAAHDA
                     IVEAHGALKTVACSLMKIANDIRLLASGPRCGIGELHIPDNEPGSSIMPGKVNPTQCE
                     AMTMVAAQVLGNDVAINIGGATGHFELNVFKPVMIYNFLHSARLIGDVCVSFNDKCAV
                     GIEPIRENIEKNLMNSLMLVTSLNTKIGYYKAAEIAQTAHKKGLTLKQAAIELGHLTS
                     EQFDEWVKPEEMVGENK"
BASE COUNT          337 a          525 c          540 g          467 t
ORIGIN      
        1 catcgtcaat ttcccgaaag acaaactggc cgtcatccag ggcctggaga attacatcgt
       61 cgtgtatgcc gacgatgtct tgctcatctg ccccaaagac gacgaacaac aagtgcgcaa
      121 cctggtgaat gatgtgaaga tcaagaaggg ggataagttc gtttgatatc cttgccatat
      181 cgatgaaata agaaaggctg tcgcgaatcg acagcctttc ttaattcatt cgtctccggg
      241 atcacttatt ctcccccacc atctcttccg gcttgaccca ttcgtcaaac tgttcggagg
      301 tgaggtgccc gagttcgatc gcggcctgct tcagcgtaag gcctttcttg tgcgcggtct
      361 gcgcgatctc ggctgctttg tagtaaccga tcttggtgtt gagcgacgtc accagcatga
      421 gcgagttcat gaggttcttc tcgatattct cgcggatcgg ttcgatgccg acggcgcatt
      481 tgtcgttgaa ggaaacgcag acgtcgccga tcaggcgggc ggagtgcagg aagttataga
      541 tcatcaccgg cttgaacacg ttcagctcga agtgtccggt ggcgccgccg atgttgatcg
      601 ctacatcgtt gcccagcacc tgcgcggcga ccatggtcat ggcctcgcac tgggtcgggt
      661 tgacctttcc cggcatgatg gaagagccgg gctcgttgtc cgggatgtgc agttcgccga
      721 tgccgcagcg cgggcccgag gccagcaggc ggatgtcgtt ggcgatcttc atcagggaac
      781 aagcgactgt tttcagggca ccgtgggctt ccacgatcgc gtcgtgcgcg gccagcgctt
      841 cgaacttgtt ctcggcggtg acgaacggca ggccggtcag cttcgcgatg tgcttcgcta
      901 cgttgacatc gtagcctttg ggcgtgttga tccccgtacc gacggccgtt ccgcccagtg
      961 ccagttcggc caggtggggc agcgtatgcc ggatcgcgcg caggccgtgt tcgagctggg
     1021 agacgtaacc ggagatctcc tgtccaagcg tcagcggggt cgcatccatg aggtgggtac
     1081 gaccgatctt cacgatgttc ttgtattcct tcgccttcgc cgcgagggtg tcgcgcagct
     1141 tctcgatgcc cgggatggtc acatccatca ggatcttgta ggcggcgatg tgcatggccg
     1201 tcgggaaggt atcgttcgac gactgcgact tattcacgtc gtcgttcggg tgcacggctt
     1261 tcttctcgtc ggtgagctga ccgccgctga tgacgtgtgc gcggtaggcg accacctcgt
     1321 tcacgttcat gttcgactgc gtaccggagc cggtctgcca cacgaccagc gggaactgat
     1381 cgtccagctt acccgccagg atctcgtcgc agacctgtcc gatgaggtcg catttctctt
     1441 tgggtaatac gccggcatca aggttagcca aagccgcagc ttttttgagg taggcgaacg
     1501 cgcgcacgat ctccttcggc atgcggttga tatcctgcgc gatccggaaa ttatcgatcg
     1561 aacgctgcgt ctgagcgccc cagtaaacat tcgcgggcac ttccacgaca cccatggtgt
     1621 ctttttcttt acggtaggat tgcatggttt ggtgttccgg gtttcgggtt tcgggtttcg
     1681 agtttcgagt tgctgaaccc gataacccat ctacttttta gtgctcacga taagctattg
     1741 actaatcgct attggctcaa ctcagcttac tgtttactgt tcactgttca ctcccgccta
     1801 cgctaaagct ccggcgggca ggattcacta ttcactattc gctattcgct attcgctatt
     1861 cgctcccgc
//
LOCUS       DAOTON010000615         1862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_48532_length_1862_cov_3.650802, whole genome shotgun sequence.
ACCESSION   DAOTON010000615 DAOTON010000000
VERSION     DAOTON010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48532_length_1862_cov_3.650802"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="P5292_11025"
     CDS             complement(<1..595)
                     /locus_tag="P5292_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39703.1"
                     /translation="MLARTHIARFYGISLAFVAFCIWMLTKELYWVAAIPALLLVLYF
                     AVYSIDKILWIVVFSTPLAIVLNDKSTGPALSMPTEPLLFGVLLLFTFKFIFEGGFDR
                     RILYHPVTVSIGFLVLWKFITSLTSSMPGVSFKHLVSQLWFIVPMYLLGTQLFRDKKN
                     IKRFIWLYSIPLLIVVGYTIVRHAQNGWTQQAAHWVMI"
     gene            complement(608..1561)
                     /locus_tag="P5292_11030"
     CDS             complement(608..1561)
                     /locus_tag="P5292_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39704.1"
                     /translation="MNNSFFNSLELFWLLNQWRKHLIIVGLISLVASAIFSSPTFIPP
                     RFKSYAIVYPSNLIAYSTESATEQMLQIAQSSEIRDRVINTFDLYKHYDIDTAKNDHF
                     RTDVIKQYEENVTIKQTEYESMEITVFDTDPMIASRMVDSIIHYFDVKAREMQAEKSA
                     EVLVIAKEQLDKKQAEIDSLEARVKEFRMNYNLLDYKAQSVEAYRGYFRNLASGNSRG
                     ASEAKAIIESLKDKGGEFYAMNENLWRYRGTFNDLKVEYDNAYRDVFKKLTYANVVTS
                     PQPADKKSYPIRWLIVLISVSASTFVAFLVLLIWNPKKSNS"
     gene            complement(1580..>1862)
                     /locus_tag="P5292_11035"
     CDS             complement(1580..>1862)
                     /locus_tag="P5292_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39705.1"
                     /translation="LLARYGGASVSLTEQLTLDREELAKMKEQVDKLRLDANQQLSHK
                     FLVNKAVQAEKKSYPVRWMIVLMAGLGGFLIALLVLLGTERYREIRYRI"
BASE COUNT          426 a          456 c          549 g          431 t
ORIGIN      
        1 ggatcatgac ccaatgcgcc gcctgttgcg tccagccgtt ttgcgcgtgg cgtacgatgg
       61 tgtagccgac gacgatcaat aatggaatcg aatagagcca gataaaccgt ttgatattct
      121 tcttgtcacg gaataactgg gtgcccagca ggtacatcgg aacgatgaac cataattggg
      181 aaaccagatg cttgaacgat acacccggca tggaactcgt cagcgaggtg atgaatttcc
      241 ataggaccag gaacccgatg gagacggtca ccggatggta gaggattcga cggtcgaagc
      301 cgccttcgaa gatgaatttg aatgtaaaga ggagcagtac gccgaacaat aaaggttcgg
      361 tcggcatgct cagtgccggc ccggtcgatt tgtcgttcag gacaatggcc aggggcgtgc
      421 tgaacacgac gatccacagg atcttatcga tcgaatagac cgcgaaatag agtaccagca
      481 gcaacgcggg gatcgcggca acccaataga gctccttggt cagcatccag atacagaatg
      541 ctacaaaggc gagcgagatc ccgtaaaatc gggcgatgtg ggtcctggcg agcaagcggg
      601 ttcagaatca ggagttcgac tttttcgggt tccagatgag caacacgagg aacgcgacaa
      661 aggtggaagc gctgacggaa atcaggacga tcagccaacg gatcggatag gactttttat
      721 cggccggctg cgggctggtc acgacgttgg cataggtgag ctttttgaag acatcccggt
      781 aggcgttgtc gtactcaact ttcaggtcgt tgaacgttcc gcgataacgc cagaggttct
      841 cgttcatggc gtagaactct ccacccttgt ctttgagtga ttcgatgatg gctttggctt
      901 ccgaagcgcc gcgcgaattg ccggaagcaa ggttgcggaa gtatccgcgg taggcttcca
      961 ccgattgggc cttgtagtcg agcaggttgt aattcatccg gaattccttc acccgtgctt
     1021 caagggaatc gatctccgcc tgtttcttgt cgagctgttc cttcgcgatc acaagcactt
     1081 ccgccgactt ctccgcctgc atttcacggg ccttgacgtc gaagtagtgg atgatcgaat
     1141 cgaccatgcg ggaggcgatc atgggatcgg tgtcgaacac cgtgatctcc atggattcgt
     1201 attccgtctg cttgatggtt acgttctctt catactgttt gatgacatcc gtgcgaaagt
     1261 gatcgttctt tgcggtgtcg atatcgtaat gcttgtagag gtcgaacgtg ttgatcacac
     1321 ggtcacggat ctccgacgac tgcgcgatct gaagcatctg ttccgtggcg ctttcggtcg
     1381 agtaggcgat caggttcgac gggtagacga tcgcgtagga cttgaaccgc ggtggaatga
     1441 aggtcgggga tgagaagatc gccgaggcga ccaggctgat cagacctacg atgatcaggt
     1501 gtttccgcca ttggttcaac agccagaaca gttccagcga attgaagaag gaattattca
     1561 tatcgattgt tcggttgcat cagatgcggt agcggatttc gcggtagcgt tccgtaccca
     1621 gtaataccag cagggcgatc aggaatccac ccagtcctgc catcaggacg atcatccagc
     1681 ggacagggta ggacttcttc tcggcctgaa cggctttgtt cacaaggaac ttatgcgaaa
     1741 gttgctggtt ggcgtcgagt cggagtttgt cgacctgctc tttcattttc gcgagctcct
     1801 cgcggtcgag cgtcagttgc tcggtcagcg ataccgaagc gccgccatag cgggccagca
     1861 gg
//
LOCUS       DAOTON010000616         1859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_48645_length_1859_cov_3.430710, whole genome shotgun sequence.
ACCESSION   DAOTON010000616 DAOTON010000000
VERSION     DAOTON010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48645_length_1859_cov_3.430710"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..529)
                     /locus_tag="P5292_11040"
     CDS             complement(146..529)
                     /locus_tag="P5292_11040"
                     /inference="COORDINATES: protein motif:HMM:NF027575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HRS39706.1"
                     /translation="MQKSLLIILVMLAGYACNPGGSVSGEGKAEDPVRPPATSRPEPA
                     EAGVWSVRTFSNSDGNSGYGFDILNGEKIYVHQPTIPAVQGVRGFSSEANAKACGELM
                     VYKIRNGILPPSVSVAELDSLSVSR"
     gene            complement(539..>1859)
                     /locus_tag="P5292_11045"
     CDS             complement(539..>1859)
                     /locus_tag="P5292_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HRS39707.1"
                     /translation="VRDTTMRRKAWEASKAVGAELKPGLMNLRDLRNKVVEGLGYPDY
                     FTYQVSDYGMSTDEMMNLLDRFNKELYPLYRELHTYMRYELSKKYGVKNVPEFLPAHW
                     LSNRWGQDWSEMVTVEGLNLDSALQLKDAEWVARQGEKFYVSLGFPELPKSCWDKSSL
                     YPVPKDAGYKKNTHASAWHMDLENDVRSLMSIVPNAEWYETVHHEYGHIYYYISYSNP
                     EVPYLLREGANRAYHEALGSMMGLAAMQQPFAAGLGLVDSTTKMDEMKHLLKDALNYV
                     VFIPWSAGTMSHFEYELYAKRLPSDQWNKRWWEIVRQYQGIVPPVERGEDYCDAATKT
                     HINDDPAQYYDYALSFILLFQVHDHIARNILKQDPHATNYFGNKEVGKFIADIMRPGS
                     SADWRKLLKEKTGEDLSARAMLDYFSPLMDWLKKENAGRTYTLPEMK"
BASE COUNT          412 a          516 c          463 g          468 t
ORIGIN      
        1 ccgaagtcgg cgaaagcctg caactccgtt accagttctg gaaccatctc cactatcagg
       61 tcaaatgcta tcgggaatac cttgcacttg cctattataa aataaaaggg tggatctgag
      121 tccacccttt tatctatgct tgtaattatc gggaaacgct cagggaatcc agctccgcga
      181 ctgaaacgga cggcggaagg attccattgc ggatcttata gaccatcagt tcgccgcagg
      241 ctttcgcatt cgcttctgaa gagaaaccac gaacgccctg taccgcggga atcgtaggtt
      301 ggtgcacgta gatcttttca ccgttcagga tatcaaatcc gtagccggaa tttccgtctg
      361 aattggaaaa ggtccgaacg ctccaaacac ccgcctcagc aggttcgggt cgggatgttg
      421 ccggcggacg cacgggatct tcggcttttc cctccccgga aacagaccct ccgggattgc
      481 atgcgtaacc tgcaagcatg acaagtatga tcaacagact tttctgcatc gcggcctgtt
      541 atttcatttc cggtaacgta taggtccggc cggcgttctc ttttttcaac caatccatca
      601 gcggactgaa atagtccagc atggcgcggg cgctcagatc ttctcccgtc ttttccttca
      661 gcaacttacg ccagtcggca ctgcttcccg gccgcatgat gtcagcgatg aatttaccga
      721 cttccttgtt gccgaaataa ttcgtcgcgt ggggatcctg tttgaggatg ttcctggcga
      781 tgtgatcgtg cacctggaac agcaggataa aactgagtgc atagtcataa tactgtgcag
      841 ggtcgtcgtt gatgtgggtc ttggttgccg catcgcaata gtcttcaccc cgctcaaccg
      901 gcggcacaat gccctggtac tgtctgacga tctcccacca gcgcttgttc cactgatccg
      961 aaggaagacg cttggcgtac agttcatatt cgaaatgact catcgtccct gcgctccagg
     1021 gaatgaagac gacatagttt aaggcatcct tgagcaggtg tttcatctca tccatcttgg
     1081 ttgtggaatc aaccaggcca agtccggcgg caaagggttg ctgcatggct gcaagaccca
     1141 tcatgctgcc aagcgcctcg tgatatgccc ggtttgcccc ctccctcagg aggtaaggta
     1201 cttcaggatt gctgtaagaa atgtaataat agatgtgccc gtattcatga tgcaccgtct
     1261 cgtaccattc ggcattcggc acgatactca tgagcgaccg aacatcgttt tccaggtcca
     1321 tgtgccaggc ggaagcgtgg gtgttctttt tataaccggc atctttcgga accggataca
     1381 ggctggattt atcccagcat gatttcggaa gttccggaaa gccgagtgaa acatagaact
     1441 tctcaccctg acgggcgacc cattcggcat ctttcagctg tagcgcgctg tcgaggttga
     1501 gtccttccac ggtgaccatc tcactccagt cctgtcccca acgattgctc agccaatgcg
     1561 caggcaggaa ttccggtaca ttttttaccc catacttctt tgaaagctca tagcgcatgt
     1621 acgtatggag ctcacggtac agcggataaa gttccttgtt gaagcgatcc aacaggttca
     1681 tcatctcatc ggtcgacatt ccgtaatcgg aaacctggta ggtgaaataa tcggggtagc
     1741 cgagtccttc caccactttg tttcgcaggt cgcgcaggtt catcagcccg ggcttcaact
     1801 ccgcgcctac cgccttgctg gcctcccagg ccttgcggcg catggtcgta tccctgaca
//
LOCUS       DAOTON010000617         1856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_48761_length_1856_cov_2.727374, whole genome shotgun sequence.
ACCESSION   DAOTON010000617 DAOTON010000000
VERSION     DAOTON010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48761_length_1856_cov_2.727374"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="P5292_11050"
     CDS             <1..471
                     /locus_tag="P5292_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRS39708.1"
                     /translation="NLEKLKRLHADFGWNLLLNEHTFIERNGDRIGLLGVENWSARAN
                     FSRYGDLGKALNGFTPAPVNILLSHDPSHWKAEVVNHPFIDLTLSGHTHGFQFGVEIP
                     GFRWSPVQYVYKEWADLYDAGRQHLYVNRGLGFLGYPGRVGILPEITVFEFSRA"
     gene            468..1256
                     /locus_tag="P5292_11055"
     CDS             468..1256
                     /locus_tag="P5292_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRS39709.1"
                     /translation="MSMLSNNQLKWVRSLHQKKFREEEGCFLAEGYKVVEEALQTGMT
                     VSLIAGTPEFLHRMKSHPAASAGIEFVECTRQQLDRMSSLSTAPDGLAVIRKPATSHD
                     EVVPSDHLVLVLDGIRDPGNLGTILRIADWFGLPSLVVSEDTVEEFNPKVVQAAMGSL
                     FRTKIRRTRLDTFLAAYSAATAHPVLAATMDGPDLFKSRFPKSACLVLGNESEGIRPE
                     VLSCCTERISIPGYFKSGQGPESLNVAVSSAVLLAEYRRVHPDQ"
     gene            complement(1257..>1856)
                     /locus_tag="P5292_11060"
     CDS             complement(1257..>1856)
                     /locus_tag="P5292_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRS39710.1"
                     /translation="PGGIIAPAATAQGEVVVSGWSPSKRNNPYANSGMVVSVGLADFL
                     PYDAAGALSGLRFQAEVEQQAFRAGGGKLVAPAQRLIDFCERKASRDLPDCSYLPGIR
                     PVKLDEVLPTTVSRALREGLKQFGKKMKGYFTNEAVLVATESRTSSPVRIPRDRETLM
                     HPQIAGLFPCAEGAGYAGGIVSAAMDGERCAEQLVKRRG"
BASE COUNT          393 a          509 c          527 g          427 t
ORIGIN      
        1 aaccttgaaa aactcaagcg tctccacgcc gacttcggct ggaacctgtt gctcaacgag
       61 catacgttca tcgaacgaaa tggcgatcgc atcggactgc tgggcgttga gaattggagc
      121 gcgcgcgcga acttctcccg ctacggcgac cttggtaaag cgttgaacgg ctttacgccc
      181 gcgccggtca acatcttgtt atcgcacgat ccttcccatt ggaaagccga ggtggtgaat
      241 catccgttca ttgacctgac cctttccgga catacccatg gcttccaatt cggtgttgag
      301 atccccggtt tccggtggag cccggttcaa tatgtgtata aggagtgggc cgatttgtat
      361 gatgccggac gacagcattt gtatgtgaac cgcggcctcg gatttctcgg ttatccggga
      421 cgtgtcggta tcttgccgga gattaccgta tttgagttca gccgcgcatg agcatgctct
      481 ccaacaatca gttgaagtgg gtccggtccc tgcaccagaa gaaattccgg gaggaggagg
      541 gatgcttcct ggccgagggt tataaggtgg tggaagaagc cttgcagacc ggaatgacgg
      601 tcagcctgat tgccggtaca cctgaattcc tgcaccggat gaagtcgcat cctgctgctt
      661 cggccggaat cgaatttgtg gaatgtaccc gccagcaatt ggaccgcatg tcgagcctga
      721 gtacagctcc ggacggcttg gcggttatcc ggaagcctgc aacttctcat gacgaggttg
      781 ttccgtcaga tcatcttgtg ttggttttgg atggaatacg agatcccggg aacctgggaa
      841 ccatcttgcg tattgcagat tggttcggtt taccctcgct tgtcgtatca gaggatacgg
      901 tggaagagtt caatcccaag gtcgtgcaag cggcgatggg atcgctcttc agaaccaaga
      961 tccgtcgcac gaggctggat acctttcttg cggcatacag cgccgcaacc gctcatccgg
     1021 tactcgcggc aacgatggat ggaccggact tattcaaaag ccggtttccg aaaagtgcct
     1081 gcctggtatt gggaaatgaa tcggagggaa ttcgtccgga ggtcttaagt tgttgtacgg
     1141 aacgaatctc cataccggga tacttcaaga gcggacaggg ccccgaatcg ctcaatgtag
     1201 cagtaagttc cgctgtgctg ctggccgaat accggcgcgt gcatccggat cagtgatcag
     1261 cctcttcttt tgaccaattg ctcggcacag cgctcaccgt ccatggccgc tgacacgatt
     1321 ccaccggcat atcccgctcc ttcggcacaa gggaagagtc ccgcgatttg cggatgcatg
     1381 agcgtttccc ggtctctcgg aatccgcaca ggcgaagagg tccgggattc ggttgcgacc
     1441 agcacggctt cgttcgtaaa gtagcctttc atcttcttgc cgaattgctt cagtccttcg
     1501 cgtaacgcac gggacaccgt ggtgggtaat acttcgtcga gctttaccgg acgaattccc
     1561 ggtaagtaag agcaatcggg cagatcgcga cttgccttgc gttcacagaa atcgatcagg
     1621 cgctgggcag gcgccaccaa ttttcctcct ccggcccgga aggcttgctg ttcaacttcg
     1681 gcttgaaatc gcaagcccga caatgcgcca gcagcatcat agggaagaaa atccgctaac
     1741 ccaacgctta ccaccatgcc ggaattcgcg taggggttat tgcgtttgga aggtgaccag
     1801 ccgctgacca ccacttcacc ctgcgcggtg gcggccggtg cgatgatccc acccgg
//
LOCUS       DAOTON010000618         1846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_49128_length_1846_cov_4.972083, whole genome shotgun sequence.
ACCESSION   DAOTON010000618 DAOTON010000000
VERSION     DAOTON010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49128_length_1846_cov_4.972083"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1355)
                     /locus_tag="P5292_11065"
     CDS             complement(66..1355)
                     /locus_tag="P5292_11065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRS39711.1"
                     /translation="MRLLNITNRYYLVTSVVLLLVSSAFLSYRVFYVVDKEIAEHMLI
                     KKSRIEEQIASQPFLQDRPFQFGDLVEVTPIERFTSFRVYLKDTALYDAYEEDLVPFR
                     VLSYEQQINKKAYRIRIWDRGTQNKELWRGMVYTILLVGIDIVALFYFLNRFFSTRIW
                     RPFYQAIAMLQRFDVQRNSKLRFLFSNVDEFNTLNNELIRLTDKVTRDYRNLREFTEN
                     MSHETQTPLAIIRSKLEIIMQSDNLTPEQMNQVRSALDSVNRLSKMNRGLILLTRIEN
                     EQYAEEQHVPLGKLIRKQLGDLDMFIESRELKVRDEIDERVFLLMNPYLADILITNLL
                     SNAIKYNKEGGELLVRLTASELVVGNSGDPLPIPGSQIFERFKKGNQADSVGLGLAIV
                     KRICDHSELEVHYDYLDDRHLFILRFSAERVFQSDRA"
     gene            complement(1352..>1846)
                     /locus_tag="P5292_11070"
     CDS             complement(1352..>1846)
                     /locus_tag="P5292_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS39712.1"
                     /translation="VIEEMQKKEHQTSILIVSARNSLDDRLTGLNIGADDYITKPFHM
                     PELGARIKSVFRRRSSQGRRELVFHELKVVPDEMMMYVNDKMISLTKKEHELLMYLVS
                     NPNKVLSKEAIAEHLWGDHADMADSFDFIYSHIKNLRKKIIDSGGRDYIKSVYGVGYK
                     FTGE"
BASE COUNT          373 a          495 c          524 g          454 t
ORIGIN      
        1 attcctcggt cccgcaactc gttccttcat tccgtccctt gtcccttgcc cctcgtccct
       61 gatcttcacg ccctgtcgga ttggaagacg cgctcggccg agaagcggag gatgaacagg
      121 tgccggtcgt cgaggtaatc gtaatgaact tccaattcgg aatggtcgca gatgcgcttt
      181 acgatggcca ggcccagtcc gacggaatcg gcctggttgc cttttttgaa tcgttcgaag
      241 atctgactgc ccgggatcgg caatggatcg ccgctgttgc cgaccaccag ttccgaggcg
      301 gtaaggcgga cgagcagctc ccctccttct ttgttgtact tgatggcgtt cgagagcaga
      361 ttggtaatca ggatgtcggc caggtaggga ttcatcagca ggaacacccg ttcgtcgatc
      421 tcgtcgcgca ctttcagttc ccttgattcg atgaacatat cgaggtcgcc cagttgcttc
      481 cggatcagct tgccgagtgg tacgtgttgt tcctccgcat attgctcgtt ctcgatacgc
      541 gtaagcagga tgagtccgcg gttcatcttc gacaggcgat tcaccgaatc gagcgcgctg
      601 cggacctggt tcatctgctc cggcgtgagg ttatcggact gcatgatgat ctccagcttg
      661 gagcgaatga tcgcgagcgg tgtctgcgtt tcgtgcgaca tgttctccgt gaattcccga
      721 aggttgcggt agtcgcgggt caccttgtcg gtgaggcgga tgagctcgtt gttcagcgta
      781 ttgaactcgt cgacattgga aaacaggaat cgcagtttac tgttgcgctg cacgtcgaat
      841 cgttgcagca tagcgatggc ctggtaaaaa ggtcgccaga tacgtgtcga gaaaaaacgg
      901 ttgaggaagt aaaacaacgc gacgatgtcg atgccgacca gcaggatggt gtacaccatg
      961 ccgcgccaca gttctttgtt ctgcgtgccc ctgtcccaga tgcggatccg gtacgccttc
     1021 ttgttgatct gctgttcata cgagagcacc cggaacggga ccaggtcctc ttcataggcg
     1081 tcgtacaggg cggtatcctt caggtaaacg cgaaaggacg taaagcgctc gatcggcgtg
     1141 acctcgacca ggtcgccgaa ctggaacggc cggtcctgca gaaacggttg cgaggcgatc
     1201 tgctcttcaa tgcgcgattt cttgatgagc atgtgctcgg cgatctcctt gtcgaccacg
     1261 taaaacacac ggtacgagag gaacgcggag ctgaccagga gcaacaccac cgaggtaacc
     1321 aggtaatacc ggttggtgat gttgagcagc ctcattctcc ggtgaatttg taaccgacac
     1381 cgtagaccga cttgatataa tcgcgtccgc cgctatcgat gattttcttg cgcaggttct
     1441 tgatgtggct gtagatgaaa tcgaaggagt cggccatgtc ggcatgatca ccccacaagt
     1501 gttccgcgat tgcttccttg ctcaacacct tgttcggatt gctgaccagg tacatgagca
     1561 gttcgtgttc tttcttcgtc agactgatca tcttgtcgtt cacgtacatc atcatctcgt
     1621 ccggcacgac cttgagttca tgaaagacca attcccggcg gccctgggaa gaccgacggc
     1681 ggaagaccga tttgatacgc gccccgagtt ccggcatgtg gaaaggcttg gtgatgtaat
     1741 cgtcagcccc gatgttgagt ccggtcaacc ggtcgtccag tgaattccgt gcggaaacga
     1801 tgaggatact ggtctggtgt tcctttttct gcatctcctc gatgac
//
LOCUS       DAOTON010000619         1844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_49209_length_1844_cov_4.121297, whole genome shotgun sequence.
ACCESSION   DAOTON010000619 DAOTON010000000
VERSION     DAOTON010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49209_length_1844_cov_4.121297"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1271)
                     /locus_tag="P5292_11075"
     CDS             complement(108..1271)
                     /locus_tag="P5292_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HRS39713.1"
                     /translation="MMEEYFAPLEADRFAFPEGRATFGQVVRKYVEGVSFPELEGAHI
                     AIFGVGEERGTVQNAGCAAAPDAVREKLYALKTGRYAYNILDLGNLRLGATLQDTYAA
                     VASIHADLIREGILPVILGGSQDLTFAQYVAYQKLEETVNIVAVDASFDLGATEDPIG
                     SDTYLGKIVLHEPNFLFNFSNLGYQTYFTGSDQVELMEKLYFDTYRLGQVQRDMEEAE
                     PIVRNADILSFDLTAIRRSDAPGNGKATPNGFYGEEACQIVRYAGLSDKLSSFGLYEL
                     NPAFDEHGQTAHLAAQMIWYLTEGYYNRKKDVPLKSKTDFVKYRVALKRADQEIVFYK
                     SQKSERWWMEVPYPNSKVRYHRHHLVPCSYHDYEIALRDEMPERWWQAFQKLS"
     gene            complement(1416..>1844)
                     /locus_tag="P5292_11080"
     CDS             complement(1416..>1844)
                     /locus_tag="P5292_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005414607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HRS39714.1"
                     /translation="IELIEEKRQAQRDRIIRAFDGEPEIQILKGRWGPFISYKGRNLR
                     IPKDREPASLTLEEIHAIAEATPDVPRKGGFRRFGAKKTTEKETPLKKAAAAKPVARK
                     AKAPAKKAAVKKAAPVKKAAAPKKAAAKKAPAKKAAVKKK"
BASE COUNT          336 a          558 c          510 g          440 t
ORIGIN      
        1 tcctgcctac gctaaagctt cggcaggtaa aattcactat tcactattca ctatttactg
       61 tttactgttt actgtttact gttcactatt cactgttcac tgctccctca gctcaacttc
      121 tgaaacgcct gccaccagcg ctccggcatt tcgtcgcgga gggcgatttc gtaatcgtgg
      181 taggaacagg gcacaaggtg gtgacgatga tagcgcacct tgctgttggg gtacggcact
      241 tccatccacc agcgttcgct cttctggctc ttgtaaaaga cgatctcctg gtcggcgcgc
      301 ttcaacgcca cgcggtactt cacaaaatcg gtcttgctct tgagcgggac gtctttcttc
      361 cggttgtaat agccttcggt caggtaccag atcatctgcg ccgccaggtg cgcggtttgt
      421 ccgtgctcgt cgaaggcggg attcagttca tacaatccga acgaactcaa tttgtccgac
      481 agtcccgcat aacgcacgat ctggcaggcc tcttctccgt aaaatccgtt gggcgttgcc
      541 ttaccgttgc ccggcgcgtc gctccggcgt atggcggtca ggtcgaagga aagtatgtcg
      601 gcgtttcgga cgatcggttc cgcctcttcc atatcccgct gcacctggcc gaggcgatag
      661 gtatcgaaat acaatttctc catcaactcc acctggtccg agcccgtgaa ataggtctgg
      721 taaccaaggt tgctgaagtt gaacaggaag ttcggttcgt gtagcacgat ctttccgagg
      781 taggtgtcgg aaccgatggg gtcttccgtg gcgccaagat cgaaggacgc gtccaccgct
      841 acgatgttca ccgtctcttc cagtttctga taggcgacat attgcgcgaa ggtgaggtcc
      901 tggctgccgc ccaggatcac cggaagaatt ccctccctga tcagatcggc atgaatgctg
      961 gcgaccgcgg cgtaggtatc ctgcaaagtc gcgcccagcc ggagattgcc gaggtcgagg
     1021 atgttgtacg cgtaacgacc ggtcttcagc gcatagagct tttcccgaac cgcgtcgggc
     1081 gccgccgcac agccggcatt ctgcaccgtg ccccgctcct cgcctacgcc gaagatcgca
     1141 atgtgcgcgc cttccagctc gggaaaggaa actccttcca catacttgcg gaccacttgt
     1201 ccgaatgtcg cccggccttc gggaaacgca aagcgatccg cttccagcgg cgcgaaatac
     1261 tcctccatca tcgtactgcg ggttttggga tcgaccctcg cggattgaac aaatcacgat
     1321 cccgtccgtg cgcgtaaaaa tccggaatca attcccgacc gcgactacgg tcgacagcga
     1381 acaatcaaca acggaatcaa cgaagcggga tccgcttact tcttcttgac ggcagccttc
     1441 ttggccggcg ctttcttggc ggccgccttc ttgggagcgg cggccttctt taccggggcg
     1501 gccttcttca cggctgcttt cttggcaggc gctttggctt tccgtgcgac gggcttggcg
     1561 gcagcggcct tcttcagcgg cgtttctttc tcagtggtct tcttcgcacc gaaccggcgg
     1621 aagccgcctt tacgcggtac atccggcgtc gcttcggcga tcgcgtggat ctcttccagc
     1681 gtgagggaag ccggctcccg gtccttcggg atccggaggt tgcgaccttt gtaggaaatg
     1741 aagggacccc agcggccttt caggatctgg atctccggct cgccgtcgaa cgcgcggatg
     1801 atgcggtcgc gttgcgcctg tcgcttctct tcgatcaact cgat
//
LOCUS       DAOTON010000620         1843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_49260_length_1843_cov_2.614653, whole genome shotgun sequence.
ACCESSION   DAOTON010000620 DAOTON010000000
VERSION     DAOTON010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49260_length_1843_cov_2.614653"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="P5292_11085"
     CDS             complement(<1..332)
                     /locus_tag="P5292_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39715.1"
                     /translation="MRIRCPFFLLVLTAFLSGSLPGSGQPYFDLFSARHLAGPGELGS
                     PGGAKLRSEYSFVQLQAPLQVKRSPQRTIIVAPYLDRWTVRSSDTAVSLRGYGLPVNF
                     LFALDSAR"
     gene            414..>1843
                     /locus_tag="P5292_11090"
     CDS             414..>1843
                     /locus_tag="P5292_11090"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HRS39716.1"
                     /translation="MSRLLLPGLLVVFQLLAHSSSTTAQVVINEYSASNLNQFVDDHS
                     DYEDWIELYNNDPSPFPIGGYHLSDDSPQPTKYTIPAGAIIPGNGFLRFFCSGRNLTN
                     GSYYHTNFRLTQTKNNKEDIVFTDPSGVILDGLRLDVTQLGHSRGRKPDGATDWYVFT
                     NPTPSTSNNAASAYLRYAEKPDFSRFPGFYTTPDSITIVTPEPNSIIRYTTNGNLPTA
                     SSTVYTGPIPFNATMVLKALVISGDPAILPSLIEFSTFFMNVSHTFPVISVSGSQLTS
                     LANGNGSLEPKGSFEYFDLSGARAAKTYGEFNRHGQDSWVHSQRSLDFISRDEMGYNH
                     SIEQRLFQYSLRRNFQRVILRAAGDDNFPADYNPDNAGSAHVRDAYVHMLAKKGGMDL
                     DVRNAEKVIVYLNGQYWGVYDIRENPDEHDYTEFYYGQDKYHIQYILTWGATWAEYGG
                     QQALDDWADLYSYIMTHDMSDPVHYDS"
BASE COUNT          433 a          552 c          459 g          399 t
ORIGIN      
        1 catcgggctg agtcaagcgc gaatagaaaa ttgaccggta gtccgtagcc gcgcaggctc
       61 accgcggtat ctgatgaccg aacggtccat cggtcaaggt acggtgccac tatgatcgtc
      121 cgttgcggcg atcgcttcac ttgcagcggc gcttgtaatt ggacaaagga atattcagac
      181 cgcaacttcg cgccacccgg actccccagc tcaccgggac cggcaaggtg ccttgcgctg
      241 aagaggtcga agtaaggctg gccgcttccg ggtagtgaac ccgacaggaa agccgttaaa
      301 accagcagga aaaatggaca ccgaatccgc acgggcgaaa aataacgctt tcctgacatg
      361 acctagccat tcagggtcga gtcatcccgc atatttcaat aacttggggc aacatgtcac
      421 gccttcttct gcccgggctg ttagtggttt tccaactact cgcccactcc tcttccacca
      481 ctgcgcaggt cgtcatcaac gaatactccg cctccaactt gaaccagttt gtagatgacc
      541 attccgacta tgaagactgg atcgaactct acaacaatga tccttcgcct tttccgatcg
      601 gtggctatca cctgagcgat gattccccac aaccgaccaa gtacaccata ccggccggcg
      661 caatcattcc gggcaacggc tttcttcgct tcttttgttc tggccggaac ctgacgaacg
      721 gcagctatta tcatacgaat tttcgcctga cgcagacgaa aaacaacaag gaggatatcg
      781 tattcaccga cccttcggga gtaattcttg acgggttgcg tttggacgtg acgcaattgg
      841 gacactcccg tggccggaag ccggatggcg ctacggactg gtacgtcttt accaatccaa
      901 ccccgtcgac ttctaacaat gccgcttctg cctatttgcg ctatgcggag aagcccgact
      961 tttctcgttt cccgggattc tacacgacgc ctgactccat cacgatcgtc accccggaac
     1021 cgaacagtat catccgctat accacgaatg gtaatctccc gacggccagc agcacggttt
     1081 acaccggccc gatcccgttc aacgcaacca tggtattgaa agccctggtc atcagcggcg
     1141 atcccgccat cctgccgagc ttgatcgaat tctcgacttt cttcatgaac gtcagtcata
     1201 cctttccggt gatctcggtt tccggatcgc aactgaccag ccttgccaac ggaaacggct
     1261 ccctggaacc caaaggaagt ttcgaatact tcgatctttc aggcgctcgt gcggcaaaga
     1321 cgtatggcga gttcaaccgg cacggtcagg actcctgggt acacagtcag cggagccttg
     1381 atttcatctc gcgcgacgag atgggttaca atcacagcat tgagcaacgg ctctttcaat
     1441 attccttgcg ccgcaatttc cagcgcgtga tcctccgcgc cgcgggcgac gataactttc
     1501 cggccgatta caatcccgac aacgcgggca gcgcgcacgt tcgcgacgct tatgtccaca
     1561 tgctggccaa aaaaggcggg atggatctcg atgtccgcaa cgcggaaaaa gtgatcgtat
     1621 atcttaacgg acagtactgg ggcgtttacg acatccgcga aaatcccgat gagcacgact
     1681 atacggagtt ctattacggt caggacaagt accatatcca gtacatcctg acctggggag
     1741 cgacctgggc ggagtacggc ggtcagcaag cgctcgacga ctgggccgat ctctacagtt
     1801 acatcatgac ccacgacatg agcgatccgg tgcattacga ttc
//
LOCUS       DAOTON010000621         1840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_49392_length_1840_cov_2.451541, whole genome shotgun sequence.
ACCESSION   DAOTON010000621 DAOTON010000000
VERSION     DAOTON010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49392_length_1840_cov_2.451541"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="P5292_11095"
     CDS             complement(<1..535)
                     /locus_tag="P5292_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39717.1"
                     /translation="MTFNRTNRFPLFRLVCICALLVPFLLSCKKEKQDPLPISFDYFP
                     TERGRFVIFEVDSIVHAENDNNNDDSVYTFHYEVLEQIDSSFIDGSGRSAQVLKRYHR
                     LTDGDPWNLTSVWTQTLTVSAAYRFEDNITFHKLAFPISENTTWDGNDMNTLDEELYV
                     YEDYHVSRSVNGMAFDST"
     gene            complement(516..1628)
                     /gene="mnmA"
                     /locus_tag="P5292_11100"
     CDS             complement(516..1628)
                     /gene="mnmA"
                     /locus_tag="P5292_11100"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRS39718.1"
                     /translation="MSKKGRVLMAMSGGIDSSVSAVLLHDQGYEVVGITMKTWDYVSS
                     GSSKKETGCCSLDSINDARSIAVSMGFPHYILDIREEFGQHVIDNFVDEYLAGRTPNP
                     CVRCNTHIKWEALLKRADMLDCEFIATGHYAKVRRHDNGRYVVSKGLDAWKDQSYVLW
                     GVKQECLSRTLFPVGGFEKPAIRRMAAEMGFHELANKPESYEICFIPDNDYRSFLKRR
                     VEGIEEKLAGGKFINRKGEVLGQHKGYPFYTIGQRKGLDIALGEPAYVIDIQPSDNVV
                     VLGSFEELQKQEMRVRDLNLMKYASLENPLETVTRIRYKDKGTPSTLVPDGDRLKVLF
                     HAPVAAVTPGQSAVFYEGDDVVGGGFIAPADDLQSN"
BASE COUNT          427 a          496 c          467 g          450 t
ORIGIN      
        1 gggtcgaatc gaacgccatt ccgttcaccg aacggctaac gtgatagtct tcgtatacgt
       61 acaactcttc atccagcgta ttcatgtcgt taccgtccca ggtcgtgttt tctgaaatgg
      121 ggaaggccag cttatgaaag gtgatgttgt cttcgaaccg gtaggcggct gaaaccgtca
      181 gggtctgggt ccatacggag gtcaggttcc agggatcccc atcggtcaac ctgtgatagc
      241 gcttcagtac ctgtgcggag cggccggagc catcgatgaa ggatgaatcg atctgctcca
      301 acacctcgta atggaacgtg taaacggaat cgtcgttgtt gttgtcattc tccgcatgca
      361 cgatggaatc cacctcgaat atcacaaaac gaccgcgttc agtcgggaaa taatcgaagg
      421 agatgggcag cggatcctgt ttttcctttt tacacgaaag caggaacggc accagcaggg
      481 cgcagataca gaccagacga aaaagcggga aacggttagt tcgattgaag gtcatcggcc
      541 ggcgcgataa aaccgccacc taccacatca tcgccttcgt agaagacggc gctctgaccg
      601 ggggtgacag cagccacggg tgcatggaac agcaccttga ggcgatcacc atccggaacc
      661 agggtactgg gagtcccctt gtctttgtag cggatgcgcg taacggtttc caatggattc
      721 tctaacgaag cgtacttcat aaggttcagg tcacgaacgc gcatttcttg cttttggagc
      781 tcctcgaagc tacccagcac tacaacattg tcagaaggtt gaatatcaat aacataagcg
      841 ggttcaccca aagcgatatc caggcctttt cgctgcccga tcgtatagaa tggatagccc
      901 ttatgctgcc cgaggacttc acctttccgg ttgatgaatt tcccgcctgc cagcttttcc
      961 tcgatccctt ccaccctacg cttgaggaac gaacggtagt cgttgtcggg gatgaagcag
     1021 atctcgtagc tctccggttt attggccagt tcatggaagc ccatttcagc cgccattcga
     1081 cggatcgcgg gcttttcaaa tccacctacc ggaaataaag tacgcgacaa gcactcctgt
     1141 ttgacgcccc acaggacata gctttgatcc ttccatgcat ccaggccttt gctgactacg
     1201 tagcggccat tgtcgtgccg acggactttg gcataatgtc cggtcgcgat gaattcacaa
     1261 tcgagcatgt cggcgcgctt gagtaacgcc tcccatttaa tgtgggtgtt gcagcggaca
     1321 cagggattcg gagttctgcc ggccagatac tcgtcgacaa aattgtcgat cacatgctgt
     1381 ccgaattctt cccggatgtc gaggatataa tgcgggaaac ccatggagac cgcgatggag
     1441 cgcgcgtcgt tgatggaatc gagtgagcaa caaccggttt ccttctttga acttccggag
     1501 ctgacgtagt cccaggtctt catggtaatg cccaccactt cgtacccctg atcgtgcagc
     1561 aatacggcgg aaacggagga gtcgattcct ccgctcatgg ccattaagac acgtcctttt
     1621 ttactcatga atttccggaa tagccctata aacggctcaa aaacaagcta ttatttggct
     1681 ttcaacagcc tgcaaagata gcttattttt cgggttccgc gatcggggtt tccggttccg
     1741 ctacgggatt caggagaaag ccggacttat aaatttcctc ccgttccgtc ttccctgctt
     1801 cgtccagtac gatccacttg ccatctttca cgccattctt
//
LOCUS       DAOTON010000622         1839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_49424_length_1839_cov_3.260650, whole genome shotgun sequence.
ACCESSION   DAOTON010000622 DAOTON010000000
VERSION     DAOTON010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49424_length_1839_cov_3.260650"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1479)
                     /locus_tag="P5292_11105"
     CDS             complement(88..1479)
                     /locus_tag="P5292_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39719.1"
                     /translation="MRTIKVLELLHALKSYEKAAFSAYLTSPYFLPAPEYPRLLQLLE
                     TLPLSTQELPKTELYGQLFPQQPFDDQQLRYLLSDFTERLEHFFLLRQLAGEKEESAL
                     LQVEALHARETSKAYQHAARRFESIDRHESAKHFHRHYRAAEIRQEFAAQHGARDEVT
                     DYVELADNLDRFYFLRKLQLQCERINYRNVLAREFDVSLIDEITRHVLRNQFLGSPLI
                     ETYYQVLQLLSGPEPETAFQSLLQLLDAHRDRFSVEDLNNVYQYVKNHCIRRINLGDE
                     VYRERLFGIYQNILADERLMRHDYLSPWEYKNIVTLGLRLRQEAWVADFIELHLHHLP
                     PNERDNAHTYNSAMLCYYRKQYRQAVKLLQTVEFTDLYYQLDARSILLKVYFETYESE
                     SLLHHISAFKIFLRRNKLVSAYQRKIYENFIRYALKIFRSSDSESKRKALAEEIRSVD
                     HISDRAWLLEQLE"
BASE COUNT          411 a          462 c          518 g          448 t
ORIGIN      
        1 aacccgatac cacctggcag ggcgcttttc cgctatttca gagcggagaa ttgaaagtgt
       61 tcccggttcc gtccgggccc tgaccggtca ctcgagttgc tccagtaacc aggcgcggtc
      121 ggagatgtga tcgacgcttc ggatctcctc cgccagcgct ttgcgttttg attcggagtc
      181 gctgctccgg aaaattttca gcgcgtagcg gatgaagttt tcgtagatct tacgttgata
      241 ggcgctcacc agcttattgc ggcgcaggaa gatcttgaat gccgagatat ggtgtagcaa
      301 cgattcggat tcgtaggttt cgaaataaac ttttaacaga atggaccgcg cgtcgagctg
      361 atagtagagg tcggtgaact cgacggtttg cagcaacttg actgcctggc ggtattgctt
      421 ccggtagtaa cacagcatgg ctgagttata ggtgtgggca ttgtcacgtt cattcggcgg
      481 cagatggtgg aggtggagtt cgatgaagtc ggccacccag gcttcctgcc gcaggcgcaa
      541 cccgagggta acgatgttct tgtattccca gggcgagagg tagtcgtggc gcatcaatcg
      601 ttcgtcggcg aggatgtttt ggtaaatgcc gaacaaccgc tcacggtaaa cctcgtcgcc
      661 gaggttgatg cggcggatgc agtggttctt gacgtattga tagacgttgt tcaggtcttc
      721 gacggaaaag cgatcgcgat gcgcgtcgag cagttgcagc aacgactgga acgcggtctc
      781 gggttccggt ccgctgagta gttgcaggac ctggtagtag gtttcgatga gcggggaacc
      841 gagaaattga ttgcgcagca catgccgcgt gatctcgtcg atcagggaaa cgtcgaattc
      901 ccgcgcaagg acgtttcgat agttgatccg ttcgcactgc aattgcagct tgcgcaggaa
      961 gtagaagcga tcgaggttgt cggcgagttc tacgtagtcc gtcacttcat cacgcgcacc
     1021 gtgttgtgcg gcgaactcct gccggatctc cgccgcgcga tagtgccggt gaaagtgttt
     1081 cgcactttca tggcggtcga tgctctcgaa ccgtcttgcc gcgtgttgat aggccttgga
     1141 tgtttcacgc gcgtgcaagg cttcgacttg cagcagggcg ctttcttctt tttcgcctgc
     1201 aagctgacga agcaggaaga agtgttccaa ccgttctgtg aaatccgaca agagatagcg
     1261 cagctgctga tcgtcaaagg gctgttgcgg aaagagctgc ccgtacaatt cagtctttgg
     1321 caactcctgt gtcgacaaag gcaaggtttc caatagctgc agtaaccggg ggtattcagg
     1381 cgccggaagg aagtaggggg aagtcaaata cgccgaaaac gctgcttttt cgtacgattt
     1441 gagcgcatgg agcaattcaa gtaccttaat cgtgcgcatt tcagacccgg acttcctgca
     1501 tctaaactaa tccaaattcg acaaacacca aatatttagg caaataaaca gccaaaatat
     1561 atcaaattca taatatcagt actgtatcga cattcgacaa actgccattt ctgtatttgt
     1621 tccagccttc ctctgcccct acgtttgtat cggtaaatca tgtctgacga cccccgagca
     1681 aacacatacg accatgaaaa acttcctcca cctgctgctt ctcttcgtgc tgggatactg
     1741 ttgtcccatc aacacgaatg ccgcgaccta ctactgggtg ggcggttccg gtaactggag
     1801 cgactacgcc atgcactggg caaccagtag cggcggctc
//
LOCUS       DAOTON010000623         1837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_49505_length_1837_cov_3.941077, whole genome shotgun sequence.
ACCESSION   DAOTON010000623 DAOTON010000000
VERSION     DAOTON010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49505_length_1837_cov_3.941077"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="P5292_11110"
     CDS             complement(<1..504)
                     /locus_tag="P5292_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39720.1"
                     /translation="MRSLLVCIFLLFLSTAHAQWQDDFSDGEFLDQPAWTGDTADFTV
                     NSDGRLQLDAAVAGTSHLSMAVPLPSLDRMEWRFRIRLDFSPSSANYSRFYLVSDAQD
                     LEGSLHGYYLQFGEALANDAVELFRQDGTTSVSIVRGPDGMVANAFTLGVRVTRDETG
                     VWSIFLDT"
     gene            630..1622
                     /locus_tag="P5292_11115"
     CDS             630..1622
                     /locus_tag="P5292_11115"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRS39721.1"
                     /translation="MKVAVVGATGLVGQTMLKVLEQRNFPVTELVPVASEKSVGKTIQ
                     WKGKSFTVRSMTDAIAEKPVLALFSAGGTTSLEWAPRFAEAGITVIDNSSAWRMDPDK
                     KLVVPEINGDVLTKEDRIIANPNCSTIQMVMALQPLEQHYGIERIIVSTYQSVSGTGK
                     KAVDQLMNERKGVSGDMAYKYTIDLNLIPQIDSFLDNGYTKEEMKMVNETRKILRSPG
                     IRITSTTVRVPVKGGHSESVNVELKKDFSLDAVRAQIAAMPGVVVVDDPANARYPMPI
                     EAEGKDEVFVGRIRRDESQPNSLNLWIVSDNLRKGAATNAVQIAEYLVAKKIVG"
BASE COUNT          438 a          528 c          515 g          356 t
ORIGIN      
        1 agtgtccagg aagatactcc agacacccgt ttcatcgcgt gttacgcgaa cgccgagggt
       61 gaaggcgttg gcgaccatgc cgtccgggcc ccggacaatc gacacgctgg tcgtgccatc
      121 ctgacgaaag agctccacgg catcgttggc aagggcttct ccgaactgta aataatagcc
      181 gtgcaacgaa ccttccaggt cctgcgcgtc cgagacgaga tagaaccggg aataattggc
      241 cgaggaagga gaaaagtcca ggcggatcct aaatcgccat tccattcggt ccagcgaagg
      301 gagtggtact gccatggaca aatgactggt accggccact gccgcatcca actgcaaccg
      361 tccgtcgctg tttaccgtaa agtccgcggt atcgcccgtc caggccggct ggtcgaggaa
      421 ctccccatcc gaaaaatcat cctgccactg tgcgtgggct gtggatagga aaagtagaaa
      481 aatgcagacc agcaggcttc gcatccggta cggctgttta atttggccct gcgaaatacg
      541 cactgcacgg gtcggatcac aaccgcatac acgcggtgta aaacaataac ccgcattcat
      601 tcgaatcaga accttagaac tcctccctaa tgaaagtagc tgtcgtcggc gcaaccggcc
      661 ttgtcggtca gaccatgttg aaagtcctcg agcaacggaa cttccctgtt accgagctgg
      721 taccggtcgc gtcggagaaa tccgttggca agacgatcca gtggaaagga aaatccttta
      781 cggtacgctc catgaccgac gccatcgcgg aaaagccggt cctggctttg ttctcggccg
      841 gcggaacgac ctcgcttgag tgggcgccca ggttcgccga ggcgggtatc acggtcatcg
      901 acaactcctc cgcctggcgg atggatcccg acaagaagct cgtcgtgccc gagatcaacg
      961 gggacgtgct cacgaaggag gaccgcatca tcgccaatcc gaattgctcc accatccaga
     1021 tggtgatggc cctgcagccg ctcgaacaac actacggcat cgagcgcatc attgtctcca
     1081 cgtatcagtc cgtttccggc accggcaaga aagccgtgga ccagttgatg aacgagcgca
     1141 aaggtgtgag cggcgacatg gcgtacaagt acaccatcga cctgaacctc attccacaga
     1201 tcgacagttt cctcgacaac gggtacacca aggaagaaat gaaaatggtg aacgagacgc
     1261 gcaagatcct gcgctcgccc ggtatccgta tcacctccac caccgtgcgt gttccggtga
     1321 aaggcggaca cagtgaatcg gtcaacgtcg aactgaagaa agatttttcg ctggacgccg
     1381 tgcgtgccca gatcgccgcg atgcccggcg tggtggtcgt cgatgatcct gcgaacgcac
     1441 gctacccgat gccgatcgag gcagagggca aagacgaagt gttcgtcggg cgtatccgcc
     1501 gcgacgagtc gcaaccgaac tcgctcaacc tctggatcgt ttccgacaac ctccgcaaag
     1561 gcgccgccac gaacgccgtg cagatcgcgg agtacctggt ggcaaagaag atcgtcgggt
     1621 gaaaaatgtt tcgagttccg ggtttcgagt ttcgagtttc cggtcttcgt aaaagctatg
     1681 gcaggctggc tttgggtggt gtaggagatc gaccgaaaag tccaacgtct ggaaaactca
     1741 gttcatccaa ttaattcgaa acacctttgc tgatcgcgac cttattgccg gatcagtttt
     1801 ccggttgtga aagcgccgtg ctcttccgta caacggt
//
LOCUS       DAOTON010000624         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_49681_length_1832_cov_4.355093, whole genome shotgun sequence.
ACCESSION   DAOTON010000624 DAOTON010000000
VERSION     DAOTON010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49681_length_1832_cov_4.355093"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="P5292_11120"
     CDS             <1..685
                     /locus_tag="P5292_11120"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS39722.1"
                     /translation="LAIRQAKLDLSRLLAVPGDSTLSPTDSLILDQSIRQDSVRSYLQ
                     RNPDYVAAGAQVVINEQLEREINAQRLPSLRLNGGYNFQRSQSEAGFSLINQSYGPNI
                     GLSLQVPIYNGGAFERQHRVAKLQTENARIQQQNVEQGLLSEALKTYAAYRTALEQVG
                     LQEENEQLSRRLLDILSQRFATSQATVLDLRAAQASYEATAASLANVRYTAKLAETEL
                     KRLMNRLPE"
     gene            complement(906..>1832)
                     /locus_tag="P5292_11125"
     CDS             complement(906..>1832)
                     /locus_tag="P5292_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRS39723.1"
                     /translation="TSAPLAKEQLITVRNQLTMTSGLDDLTGDPYCTLPSCLQYEADA
                     GTRWAYHNAPYTLLDTVIESATGQNFNTYFNSRIRNRIGMNGFWFPNGYNNIYGSTAR
                     SMARFGLLMLNRGIWDQDTLLHDTAYYHAMINTSQNLNKSYGYLWWLNGKQSFMLPYS
                     QFVFGGSLSPDAPSDMYSGIGKNSQVVSVIPSQQMVIIRMGNSPDTNDVSLIYVNELW
                     EELNKLFCTPTGMHDPVSRSFALYPNPANDAVMIRGAAEDTRISVYDLEGVLLKATIG
                     NTFSVRHFSPGIYLVSITLKDGSRSMHRLAIE"
BASE COUNT          441 a          477 c          489 g          425 t
ORIGIN      
        1 attggcgatc cgccaggcta agctggacct gtcgaggttg ctcgccgtac cgggcgactc
       61 gacgctttca ccgacggatt cgcttatact cgatcaaagc atccggcagg attccgtacg
      121 ctcgtatttg caacggaatc cggactacgt agcagccggc gcgcaggtgg tgatcaacga
      181 acagctcgaa cgggagatca acgcgcaacg gctcccaagt ctgcggctca acggtggtta
      241 caatttccag cgtagccaga gcgaagccgg gttctcgctc atcaaccaga gttacggccc
      301 gaacatcgga ctcagcttgc aagtacctat ctataacggc ggggcgttcg aacgacagca
      361 ccgcgtagcg aagttgcaaa ccgagaacgc ccggatacaa caacagaacg tcgaacaggg
      421 tttgttgtca gaggccttga agacctatgc cgcctatcgg acagcactcg agcaagtcgg
      481 cttgcaggaa gaaaacgaac aactctcgcg acgcctgctg gatatccttt cgcaacgatt
      541 cgctaccagc caggctaccg tactcgacct ccgtgccgca caggccagct acgaagccac
      601 agccgcttca cttgctaatg ttcgttatac agccaagctg gcggaaacgg agttgaagcg
      661 gttgatgaat cggttgccgg agtaggggag tgaacagtga acagtgaata gtgaacagta
      721 aacagtgaat agcgaatagc gaatagtaaa ctgttattga ttattaatca ccaatcacta
      781 attactaatt actagttacc agttaccagc caccagtttc tatcgccccc gctctccacc
      841 atcacttttt cactgtgatc ctctgtgtgt actcggtgtc ctttgtggtt atctggttcc
      901 cgtaatcatt caatcgccag tcgatgcatg cttcgagatc catcttttaa tgtaatactg
      961 accaggtaga tcccgggcga aaaatgccgg acggagaaag tgttccctat ggtcgctttc
     1021 agaagaactc cttccaggtc gtagacactg attcgagtat cttccgccgc tccacgaatc
     1081 attaccgcat cattagcggg attaggataa agtgcaaagg agcgactgac gggatcgtgc
     1141 atgcctgtgg gtgtgcagaa cagtttgttc agctcctccc acaattcgtt gacgtagatc
     1201 agcgagacgt cattggtgtc gggcgaattt cccatccgga tgatgaccat ctgctggctg
     1261 gggatcacgc tgactacctg gctgtttttt ccaattcccg aatacatatc ggaaggggcg
     1321 tccggagata aggaaccgcc gaagacgaac tgcgaatagg gcagcatgaa cgattgtttt
     1381 ccgttcagcc accagaggta accgtacgat ttgttcaggt tctgcgaggt gttgatcatc
     1441 gcatgatagt aagctgtgtc gtgcaacaac gtatcctggt cccagatacc gcgattgagc
     1501 atgagcaaac cgaatcgtgc catgctgcgc gccgtactcc cgtaaatgtt gttgtaaccg
     1561 ttcgggaacc agaatccgtt cataccgatc cggttccgga tacgggagtt gaagtacgtg
     1621 ttgaaattct gtccggttgc agactcgatc actgtatcca gcagcgtgta cggtgcatta
     1681 tggtaagccc agcgcgtgcc ggcatccgct tcgtattgca gacaggaggg tagcgtgcaa
     1741 tacggatcgc cggtgagatc gtcaagaccg gaggtcatgg tcaattggtt gcgcactgtg
     1801 atcaattgct ctttcgcgag cggtgcgctg gt
//
LOCUS       DAOTON010000625         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_49684_length_1832_cov_3.704558, whole genome shotgun sequence.
ACCESSION   DAOTON010000625 DAOTON010000000
VERSION     DAOTON010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49684_length_1832_cov_3.704558"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="P5292_11130"
     CDS             <1..964
                     /locus_tag="P5292_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39724.1"
                     /translation="VPTQPLTATAAGTDVTCFGDADGSVTATPLDGVAPYHYLWSPTG
                     DTTAAIAGLPPDTYICVITDEAGCSTSVTQSLIEPDQLVVSITLINDPTTCGGTDGNI
                     SSTVSGGTSSYDYQWNSGETSADLNNVGAGSYSVTVTDANGCSETTGVLTLNDPPLPV
                     VTLSLSTDSLCTTDPPLLLDGNTPTGGSFSGMGVSGNSFDPSIGAGDYPVLYSYTDAN
                     NCTANASDTMHVELCAGIGTITESEVLFRILPNPNRGMFRIAMTNGGAEAIVRVTNAL
                     GQTVASYRVQGDSYYVDESRLAPGIFVITTEQAGRRQHLRMVIE"
     gene            complement(1092..1598)
                     /locus_tag="P5292_11135"
     CDS             complement(1092..1598)
                     /locus_tag="P5292_11135"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRS39725.1"
                     /translation="MGTFPQELKKCVIAVAPHTSNWDVVVGMATRSVMPIRGAKFLGK
                     QELFRGILGPFMRWIGGVPVDRSKKSNTVEQVAEACRKSERFILAIAPEGTRKRVDSL
                     KTGFWHMAKAAGIPIVLAGLDYKHKAVVFSEPFHPQNEAEDFKRIYAFFSGIEGRHPE
                     LGLGHLAG"
     gene            complement(1735..>1832)
                     /locus_tag="P5292_11140"
     CDS             complement(1735..>1832)
                     /locus_tag="P5292_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRS39726.1"
                     /translation="ATDIEVGLLLNFGPKPQFTRKVFSNSRKRSA"
BASE COUNT          400 a          524 c          493 g          415 t
ORIGIN      
        1 agtcccgaca caaccgctga cagccacggc cgccggtacg gacgtgacct gtttcggcga
       61 cgccgatggt tccgtcaccg caacaccttt ggacggtgtc gctccatatc attacctctg
      121 gagtccgacc ggagacacga cagcggccat cgctggcttg cctcccgata cctacatctg
      181 cgtgatcacc gacgaagctg gctgctcgac aagtgttacc caatccctaa tcgagccgga
      241 ccaactcgtc gtttccatta cactgatcaa tgatccgacc acttgcggcg gtacagatgg
      301 taatatcagc agcacggtga gtggcggtac cagttcctat gactatcaat ggaacagcgg
      361 tgaaacttcc gccgatctca acaatgtcgg cgccggatct tactccgtca cggtcacgga
      421 tgccaatggt tgctcggaaa ctaccggggt gctgacactg aatgatccgc cgttaccggt
      481 ggttaccttg agcctctcaa ccgattcact ctgcacaacg gatccgcctc tccttctcga
      541 cggcaacaca cccaccggtg gcagcttctc aggcatgggt gtttcgggca acagtttcga
      601 cccatccatc ggcgctggtg attaccctgt cctgtacagc tatacggatg ccaacaactg
      661 taccgcgaac gcaagcgaca cgatgcatgt ggagctttgt gccggtatcg gtaccattac
      721 cgagtcggaa gtactctttc gcatcttgcc taatccgaac cgtggcatgt tccgcattgc
      781 aatgactaat ggaggcgccg aagcgatcgt gcgtgtgacc aatgcattgg gccagacagt
      841 agccagctat cgcgtgcagg gcgattcata ttacgtcgac gaatcccgct tagcacctgg
      901 tattttcgtt atcacgaccg aacaggctgg gcgccggcag catcttcgga tggtgatcga
      961 ataagaacgt tggcaacaaa atattggaga ggctgtccgg agaacccgaa cagcctcttt
     1021 gcttattttc agttcggccg aagcggtaaa gtaggttagc gagatgacga atgggtagct
     1081 cgcgcggcta tctatccagc caggtgtcca agcccaagtt cgggatgcct gccttcgata
     1141 ccggagaaga aggcgtagat gcgtttgaaa tcttccgctt cgttctgcgg gtggaatggt
     1201 tcggaaaaaa cgacggcttt gtgcttatag tcgaggccgg ccagcacgat cgggattccc
     1261 gcggccttgg ccatgtgcca gaagccggtc ttgagcgagt cgacacgctt tcgggttcct
     1321 tccggggcga tggcgaggat gaaccgctcg ctctttcggc aggcttcggc tacctgttcg
     1381 acggtattgg atttcttcga acggtcgacg ggcactccgc cgatccagcg catgaagggt
     1441 ccgaggatgc cgcggaagag ctcctgctta ccgagaaact tcgcgccgcg tatgggcatc
     1501 accgatcggg ttgccatgcc gacaacgacg tcccagttgc tcgtatgcgg cgccacggcg
     1561 atcacgcact tcttcaactc ctgcggaaac gtccccacga acttccaccc aaataccctg
     1621 agccatattt tccagaatag cctgaacatc cttggcaagt taatactcta actgctggtg
     1681 tcaaagcctc tggtcaagaa tggatcgcac gcggatcccg cggatgatgc ggatttacgc
     1741 ggatcttttt cgggaatttg aaaatacctt tcgagtaaat tgtggtttag gtccaaagtt
     1801 tagaagaagt cctacctcaa tatctgtcgc ct
//
LOCUS       DAOTON010000626         1829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_49811_length_1829_cov_2.192221, whole genome shotgun sequence.
ACCESSION   DAOTON010000626 DAOTON010000000
VERSION     DAOTON010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49811_length_1829_cov_2.192221"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="P5292_11145"
     CDS             <1..767
                     /locus_tag="P5292_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS39727.1"
                     /translation="VRNFGGRLMVYPEDAFLGSLGQVNEGVPATLSGMKGMPAIAEAV
                     MIVRDLQLVEYTGTPLHFSTISSRAGVALIRAAKKRGLPVTADVAAYSLLDNDSVLER
                     FDSHYKVKPPLRGEDDRQALIEGLSDGTLDAVASNHRPEDIEHKQLEFSLAAYGMIGL
                     ESAFGVARTATKDKVPLDRLIDAFAIAPRKVLGLELPRIEVGAEAELTVYAPDDTWSL
                     QADHLRSRSRNTPYIGRQFTGKPVAVVRGKAHYRIP"
     gene            783..1256
                     /locus_tag="P5292_11150"
     CDS             783..1256
                     /locus_tag="P5292_11150"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRS39728.1"
                     /translation="MKVLMVCLGNICRSPMAEGILRHHAEEQGWSITVDSAGTGGWHV
                     GEAPDKRAIRAAKQYGVDISQLRARQFSADDFDRFDRIFVMDRSNRSNVLALARNEED
                     RQKVDLFLHQLQPMSDAEVPDPWYGDEDGFHSVYDLLERASLAFIRKARSGTEAL"
     gene            1300..>1829
                     /locus_tag="P5292_11155"
     CDS             1300..>1829
                     /locus_tag="P5292_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRS39729.1"
                     /translation="MAIATSDFLASGPWKRPDDSSTSWRKTRSFLKAIQHPIPLQEIV
                     IAELNEHTLPESVAPLLRPVLDGHDCGIISEAGLPGIADPGAALVRLAQQKGIRVHPI
                     AGSSSLFLALMASGLNGQSFRFHGYLPRDHAARRQRLIELEKEVRRNGTTQLFIETPY
                     RNDALFDDILRTCDPSL"
BASE COUNT          412 a          538 c          505 g          374 t
ORIGIN      
        1 acgtgcgcaa cttcggcggt cgactgatgg tgtatcccga agatgctttc cttggatccc
       61 ttggccaggt caacgaaggt gtaccggcaa cgctttccgg catgaaagga atgcccgcca
      121 tcgccgaagc ggtgatgatc gtacgcgatc tccaactggt cgaatacacc ggcactcccc
      181 tccacttttc cacgatcagt tcaagagccg gtgtcgcgct catccgtgcc gccaagaagc
      241 gcggactacc ggtaaccgct gatgtcgccg catacagctt gctcgacaat gattccgtcc
      301 tggaacgctt cgacagccac tacaaagtga aaccaccgtt gcgcggcgaa gacgaccggc
      361 aggccctgat cgaaggactt tcggacggaa ccctggatgc ggtcgcttcc aatcacaggc
      421 cggaagacat cgaacacaag caactggaat tttcgcttgc agcttatggc atgatcggac
      481 tcgaatccgc ctttggcgtc gcccgaacgg caacgaagga caaggtaccg ttggatcgtc
      541 tgatcgacgc attcgccatc gcgccacgaa aagttcttgg actggagcta ccacggatcg
      601 aagtcggcgc agaagcggag ctgaccgttt acgcaccgga cgatacctgg agcctgcagg
      661 ccgatcatct tcggtcgcgt tcacggaaca cgccttacat cggcaggcag ttcaccggaa
      721 aaccggtagc cgtcgttcgg ggcaaggccc attaccggat cccttaattc aggttctgtc
      781 ctatgaaagt tctgatggtc tgcctgggca acatctgccg ctcgccgatg gcggaaggca
      841 ttctgcgtca ccacgccgaa gagcaaggat ggtcgattac cgtcgacagc gcgggaaccg
      901 gtggctggca tgttggcgaa gcgcctgata aacgcgccat ccgtgcggca aagcaatacg
      961 gtgttgatat ttcccagctt cgtgcgcgac agttttcagc cgacgatttc gatcggttcg
     1021 accggatctt tgttatggac cgatccaacc gcagcaatgt gctcgcattg gcacggaatg
     1081 aggaggaccg gcagaaagtc gatctattcc tgcaccaact ccaaccgatg agcgatgcgg
     1141 aagtgccgga tccctggtac ggcgacgaag atggcttcca ctccgtttac gatctgctgg
     1201 aacgggccag cctcgcgttc atccgaaaag cacggtcagg gaccgaagcg ctttgatcaa
     1261 ccatgtcgac cggatcactc taccttttac caactttcct tggcaatcgc gaccagcgat
     1321 ttcttagcca gcggtccctg gaagcgaccc gacgactcaa gtacttcgtg gcggaagacg
     1381 cgaagtttcc taaaggcgat ccagcatccg ataccactgc aggaaatcgt gatagcagaa
     1441 ttgaacgagc atacacttcc ggaatcggtc gctcccctgc ttcgtccagt ccttgacggg
     1501 catgattgcg gcatcatcag cgaagcggga ctgcccggca tcgctgatcc cggtgcagcc
     1561 ctggtgcgtc tggcccagca aaaaggaatc cgggtgcatc ccattgccgg atcctccagt
     1621 ttgttcctgg cactcatggc atcgggcttg aacggacagt cgttccgctt tcatggttat
     1681 ctgccgcgag atcatgccgc tagaagacag cgattgattg aattggaaaa agaagtccgg
     1741 aggaacggta ccacacaact gtttatcgaa acgccttacc gaaacgacgc gttgttcgac
     1801 gacatcttgc gcacgtgtga cccgagcct
//
LOCUS       DAOTON010000627         1821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_50114_length_1821_cov_2.885617, whole genome shotgun sequence.
ACCESSION   DAOTON010000627 DAOTON010000000
VERSION     DAOTON010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50114_length_1821_cov_2.885617"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="P5292_11160"
     CDS             complement(<1..589)
                     /locus_tag="P5292_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39730.1"
                     /translation="MEEVRKPSLQKESPEHISSELHAKKLQLHWLLQITKAINYNFTT
                     KQLLDVYEHVLSSQLKVEKLALFIKEQDWQCALLYGADKTFGEIDVAAMLDDLNRLRN
                     LETERDRWVNTFETIIPVFHKDQILAYALVGGLENSVIPRRNELIPFIQTITNLIVVA
                     IENHHIAGEKIRQAAMRRDLDLAAQMQSMLFPIALP"
     gene            complement(592..1527)
                     /locus_tag="P5292_11165"
     CDS             complement(592..1527)
                     /locus_tag="P5292_11165"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRS39731.1"
                     /translation="MKFPQPIALKALAELTGTRVIGDGSRQVSGINEIHKVEAGDITF
                     VDHPKYYDKALQSAATFVIINKEVAAPEGKALLFAEDPFSAYIRIVRHYRTFEPSTAA
                     ISPTAVIGAGTVIQPGAFIGHHVRIGNNCVIHANVSIYDHTEIGDEVIIHSNTVIGAD
                     AFYFKRRPEGYDKMYSCGRVIIHDRVEIGASCTIDKGVSGDTVIGAGTKMDNMVHVGH
                     DTVIGRNCLFAAQVGIAGVVTIEDDVILWGQVGVQKDLTIGKGAVVLGKSGVPKSLEG
                     GKTYFGSPTLEARDKMRELALIKQLPELLERLKNR"
     gene            1634..>1821
                     /locus_tag="P5292_11170"
     CDS             1634..>1821
                     /locus_tag="P5292_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRS39732.1"
                     /translation="MRIAVNARMLQKGKLEGIGYFCLETFRRITHDHPEHEFLFIFDR
                     PFDPEYVFSPNVTPIVAGP"
BASE COUNT          358 a          522 c          498 g          443 t
ORIGIN      
        1 ccggcaaggc gatcgggaac aacatgcttt gcatctgggc ggccagatcg agatcgcgtc
       61 gcatggcagc ctgccggatc ttctcgccgg cgatgtggtg attctcgatg gcgaccacga
      121 tcaggttcgt gatcgtctgg atgaacggaa tgagttcgtt cctgcgtgga atgacgctgt
      181 tctccaatcc gccgaccagg gcgtatgcca ggatctgatc cttgtgaaaa accggaatga
      241 tggtctcgaa ggtattgacc caacggtcgc gctccgtttc cagatttcga agccggttga
      301 ggtcatccag catggccgcg acgtcgattt cgccaaacgt cttatccgcc ccgtagagca
      361 aggcgcattg ccaatcctgt tccttgatga agagcgcaag cttctccacc ttcaactggc
      421 tgctgagtac gtgctcgtaa acgtcgagca actgcttggt ggtaaaattg tagttgatcg
      481 ctttggtgat ctgcaacaac cagtgtaact gcagcttctt ggcatgcaat tccgaggaaa
      541 tatgctcggg gctttccttc tgcaggcttg gtttgcggac ttcttccatg ctcagcgatt
      601 cttcagtcgt tccagcaatt cgggcaattg cttgatcagt gcgagttccc gcatcttgtc
      661 acgggcttcc agggtcgggc taccgaaata ggtcttcccg ccttccagcg acttcggtac
      721 gccggacttt ccaagtacga ccgcgccttt cccgatggtc aggtcctttt gcacgccgac
      781 ctgtccccag aggatcacgt cgtcttcgat cgtcaccacg ccggcgattc ccacctgtgc
      841 cgcgaagagg cagttgcgtc cgatcacggt atcgtgaccc acgtgtacca tgttgtccat
      901 cttggtgccg gcaccgatga ccgtatcgcc ggatacaccc ttgtcgatcg tacagcttgc
      961 cccgatctcg acccggtcgt gaatgataac gcggccgcag gagtacatct tgtcgtaacc
     1021 ttccgggcga cgcttgaaat aaaacgcatc ggcaccgata acggtattgg aatggatgat
     1081 cacctcgtct cctatttcgg tatgatcgta gatgctgacg ttggcgtgaa tgacacagtt
     1141 atttccgatc cgcacgtggt gaccgataaa agcgcccggc tggataaccg ttcccgcccc
     1201 gatgacggcc gtagggctga tcgctgccgt acttggttcg aaggtcctgt agtgtcgtac
     1261 gatccggatg taggcggaaa acgggtcttc cgcgaacagc agcgccttcc cttcaggcgc
     1321 cgcaacttcc ttgttgatga tgacaaaagt tgcagccgac tgcagcgcct tatcgtaata
     1381 ctttggatga tcgacgaagg tgatatcgcc ggcctccact ttgtggatct cgttgatgcc
     1441 cgacacctgc cgggacccgt cgccgatgac ccgcgttccc gtcagttctg ccagtgcttt
     1501 cagggcgatg ggttgaggaa acttcattga taacaaaaat agcggattcc ggcaggggac
     1561 gggcggtggt tccattcccg ttttcaacat cagggtttcc gcgctcctgc ccgagtgcgt
     1621 atcttcgccg accatgagga tcgcggtcaa cgcccggatg ttgcaaaagg ggaaactgga
     1681 aggcatcggg tatttttgcc tggaaacctt ccggcgcatc acacacgacc atcccgaaca
     1741 tgagttcctg ttcatcttcg atcgtccctt cgatccggag tatgtctttt cacccaacgt
     1801 gacgccgatc gttgcaggtc c
//
LOCUS       DAOTON010000628         1820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_50142_length_1820_cov_4.691218, whole genome shotgun sequence.
ACCESSION   DAOTON010000628 DAOTON010000000
VERSION     DAOTON010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50142_length_1820_cov_4.691218"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="P5292_11175"
     CDS             complement(<1..304)
                     /locus_tag="P5292_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39733.1"
                     /translation="MNKRNKHAPGQMAAPVKSSTSISGWSIRLLLSLLALLLYGNTLT
                     FDYALDDEVLILKNEAVQKGVSGIPEILSPGKVYENSIQPFRPVASALFAIERSLAG"
     gene            complement(359..>1820)
                     /gene="glmS"
                     /locus_tag="P5292_11180"
     CDS             complement(359..>1820)
                     /gene="glmS"
                     /locus_tag="P5292_11180"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRS39734.1"
                     /translation="LVHVIEDIFENAQVSLDEAVRMALGEVIGAYAIVVLSKDHPDQL
                     ICARKGSPIVIGVGKEKGEFFVASDATPIIEYTNNVIYLNDGELGFVNKGQLTVKTIE
                     NQVKTPYIHELELKLEQLEKGGYDHFMLKEIYEQPRSIWDSMRGRIDAQKGFLRMGGI
                     SEYEKKFANAKRILIIGCGTSWHAGLVAEYLFEELARIPVEVEYASEFRYRNPIIYED
                     DIVIPISQSGETADTLAAIELAKAKGALIFGVCNVVGSSIPRATHAGAYTHAGPEIGV
                     ASTKAFTAQVAVLTLMALQIARTRGTVNDSRFHQLLNELEALPAKVEKALKTNEQVID
                     LASKFKDARNFLYLGRGYGFPVALEGALKLKEISYIHAEGYPAAEMKHGPIALIDDEM
                     PVVVIANKNDTYEKVVSNIQEVKARKGKIIAVVTEGDKNVKELADYVIEIPETDDVLV
                     PIVSVIPLQLLSYHIAVMRGCNVDQPRNLAKSVTVE"
BASE COUNT          351 a          502 c          536 g          431 t
ORIGIN      
        1 gtccggccag gctgcgttcg atcgcgaaaa gggccgaagc gacaggccgg aaaggctgga
       61 tgctgttctc gtaaacttta cccgggctga gaatttcagg gatgccgcta acgccctttt
      121 gaaccgcttc gttcttgaga ataaggactt cgtcgtccag cgcgtaatca aacgtcagcg
      181 tgttgccgta caacagcagg gccagcaggc tcaacagcaa ccggatgctc cacccgctaa
      241 tggatgtgct ggatttgacc ggcgcggcca tttggccagg cgcgtgctta ttgcgcttgt
      301 tcatccggaa ggaaagatac gaggattccc gaactggccg ggaatgacag gcagacggtt
      361 attcaaccgt caccgacttg gccaggttcc gcggctggtc cacattgcat ccgcgcatga
      421 cggctatgtg gtaggagagc agttgcagcg gaatcaccga aacgatcggc accagtacgt
      481 catctgtttc ggggatctcg atcacatagt cggcaagttc cttgacgttc ttgtcgcctt
      541 cggtcaccac cgcgatgatc ttccctttcc gggctttcac ttcctggatg ttgctgacca
      601 ccttttcgta cgtatcgttc ttgttggcga tcacgaccac cggcatctcg tcgtcgatga
      661 gggcgatcgg tccgtgcttc atttctgcgg ctggatatcc ttccgcgtgg atgtaggaga
      721 tctccttcaa cttcaacgcg ccctcgagcg ctacggggaa accatagcca cggccgaggt
      781 acaggaagtt gcgggcatcc ttgaacttgg aagcgaggtc gatgacctgt tcgttggtct
      841 tcaacgcctt ttcaactttt gccggaagcg cttccagttc gttcagcaac tggtggaagc
      901 gggagtcgtt caccgttccg cgcgtgcggg cgatctgcag ggccatcagg gtgaggacgg
      961 ccacctgcgc ggtgaaggct ttggtggatg ctacgccgat ctccggaccg gcgtgggtat
     1021 aggcgccggc atgggtagcg cgcgggatcg aagagccgac cacgttgcag acaccgaaga
     1081 tcaacgcgcc ttttgccttg gcaagttcaa tggcggccag cgtgtcggcg gtttcacccg
     1141 attgcgagat cgggatcaca atatcgtctt cgtaaatgat cgggttccgg taacggaatt
     1201 ccgatgcgta ttcgacttcc accggaatgc gggcgagttc ttcgaagagg tactcggcga
     1261 ccagtccggc atgccaggaa gttccgcaac cgatgatcag gatgcgtttc gcgttcgcga
     1321 acttcttctc gtattcggag atgccgccca tgcggaggaa acccttctgc gcgtcgatgc
     1381 gtccgcgcat gctgtcccag atggaacgcg gctgttcgta gatctccttc agcatgaagt
     1441 gatcgtaacc gcctttttcc aactgctcga gtttgagttc gagttcgtgg atatacggcg
     1501 tcttcacctg gttctcgatc gtcttgacgg tcagttgtcc tttgttgaca aagccgagtt
     1561 cgccatcgtt caggtaaatg acgttgttgg tgtattcgat gatcggcgta gcgtcggagg
     1621 cgacgaagaa ttcgcctttc tctttgccga caccgatgac gatcggactg cctttgcggg
     1681 cgcagatgag ctggtccgga tggtctttgg aaaggaccac gatcgcgtaa gcgccgatca
     1741 cctcgcccaa cgccatgcgt acggcttcgt ccagggatac ctgggcgttt tcaaaaatgt
     1801 cctcgatcac gtgcaccaac
//
LOCUS       DAOTON010000629         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_50241_length_1818_cov_2.986387, whole genome shotgun sequence.
ACCESSION   DAOTON010000629 DAOTON010000000
VERSION     DAOTON010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50241_length_1818_cov_2.986387"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(766..1476)
                     /locus_tag="P5292_11185"
     CDS             complement(766..1476)
                     /locus_tag="P5292_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39735.1"
                     /translation="MEIGYTRLVYFFGAIALITFLGFYKKYFSLMPDFPGLKQIHHFH
                     ALALTTWLTMLIVQPILITRQKMTAHRLIGKLSYLLVPFIFLSMILVYHNQYLRMVAE
                     GKPEPETLAFVFSPATDAIPFLIFYLLAIRNKRDTPRHMRYMISTGLVIGGPGLGRIF
                     ITWMDMDLFAAIGLITLITLLVFIGLLLYDKAKKKQFNWNPYTLAFIIWLVPNILIIF
                     FPNTSLWQSFARWIVNLV"
     gene            1626..1818
                     /locus_tag="P5292_11190"
                     /pseudo
     CDS             1626..1818
                     /locus_tag="P5292_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805940.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          475 a          449 c          497 g          397 t
ORIGIN      
        1 ctggtcggcc gtgacaggca gggatacggt tactgtatgc ccgctgagga tggtgatcac
       61 gccgtcgctg ctggaaggac ccgtaagcga attgacccag gaactgccgt tgtagcgttg
      121 ccaggtggtc gtaccattcc atgccccgga accgcgcgtc tggaagtcgc cggttgccgc
      181 tgcgggtaca tccttgacat cggcaaattg aagtaggcca ccagtccgcc gatcaacaat
      241 aacgacaaag ccacagccgc ccgtcgaacg aacttctgtc gcgtacgcaa cccttgcttt
      301 agcgaagagc gcatggaagt ccgtttaggc ggattcgagc ctggggagga ggaagggaag
      361 tgtttcataa agagaaagtc gtaacagccc cgctatacac cgtttcccga tccaaagttt
      421 tccgccgcgc tcccggaaat tgccggattc aaagcaaaat tgtaagcccc aagacacttc
      481 tacaaagcca aggcacaaaa tcaatcgaaa aaagccgtta agtcaggggg gaattaaaag
      541 ttgcgggtgt aagaagagtg gtggaattgg ggactggtaa ctggtaacta gtaactagta
      601 actagtaact agtaattagt atttagtaat tggtaattag tgaatggtga cccgcttgcc
      661 tcacacaact cccgcaacca aacctaaaac caaaatcttt caactttcaa ccatcaacca
      721 tcaaccttca accttcaacc ttcaccctcc aacctccaac ggttgtcata caagattcac
      781 gatccacctg gcgaaacttt gccagaggct ggtattgggg aagaagatga tcagaatatt
      841 gggtacaagc cagatgataa aagcaagggt atacggattc cagttgaact gtttcttctt
      901 cgccttgtcg tagagtagga ggccgataaa gaccaaaagc gtgatcaacg tgatcaggcc
      961 gatggctgca aacaggtcca tgtccatcca ggtgatgaag atgcggccca agcccggccc
     1021 gccgatcacc aggccggtcg agatcatgta gcgcatgtgc ctgggcgtgt cgcgcttgtt
     1081 cctgattgct agcaggtaaa agatcaggaa cgggatggcg tccgtagcgg ggctgaagac
     1141 aaaggcgagc gtttccggct cgggcttgcc ctcagcaacc atgcgtagat attggttgtg
     1201 atagacaagg atcatggaca ggaatatgaa cggcaccagc aggtaggata gtttcccgat
     1261 cagccggtgc gcggtcattt tctggcgggt gatcaggatc ggctgtacga tcagcatggt
     1321 cagccaggtc gtcagcgcaa gcgcgtggaa gtgatgaatc tgcttcagtc ccgggaagtc
     1381 gggcatcaga gagaagtatt tcttgtagaa tccgagaaag gtgatcaggg caattgcgcc
     1441 gaagaaataa accagtcgtg tgtatcctat ttccatgttc aaagttttta tcgatatgcg
     1501 tatagcagtt ggtcatcaat accgacatca atttatatat ttgagtgtgg ctgaaaaggg
     1561 gaagccttca ctgtgccttc cccggtgagg aaccacaact ctttcgcggt ctctttgcaa
     1621 agccctgggt ggtgtacgcc aagcggccct ttgccgggcc cgaaccggtg atcgattatc
     1681 tgggtcgcta cacccacaag atcgccatca gtaaccaccg gctgctctcg gtaagcaagc
     1741 agcgcgtccg cttccggtac aaggactatc gggatggcag tacgaaaaag gtgatggagc
     1801 tatcgccgat ggagttca
//
LOCUS       DAOTON010000630         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_50248_length_1818_cov_2.733409, whole genome shotgun sequence.
ACCESSION   DAOTON010000630 DAOTON010000000
VERSION     DAOTON010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50248_length_1818_cov_2.733409"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="P5292_11195"
     CDS             <1..971
                     /locus_tag="P5292_11195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS39736.1"
                     /translation="VRLAFAATRRRSDWLIATSPFMFLIPAIAFFGRGRRKIMDVRDL
                     VWCYMPDRNIFQRAIRSLLQRVIVRSLRKFDHITVTNSSEEQWMIRSAAVPRARLSIV
                     SNGLSQDRFRRLSNIAYYPPEHPLVITYIGNIGNGQDLGPVIDVVRHLPDVKLNLIGD
                     GIELERYRKYIRQENIRNVQFFGKMKWNRLLPFYQTSSILLARLGKNYQSALPSKLFE
                     YLATGLPVLFYGNGEAARFLKDFENVFIVRQEDQEELRRAIRQLQTLTPPRSFANILR
                     VESNFIREHLNLRYLDLLKDPAERVTVLEPVGMDAQSRADWSLREA"
     gene            1040..>1818
                     /gene="cyoE"
                     /locus_tag="P5292_11200"
     CDS             1040..>1818
                     /gene="cyoE"
                     /locus_tag="P5292_11200"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRS39737.1"
                     /translation="MAQKSIALSDKPVTLSARIQDYIQLTKFRLSSLVVFSAATGYII
                     AEGADFQWSTLLLLMLGGFLVTGSSNAFNQVIERDLDKLMDRTANRPLPAGRMSVPEA
                     LVASVLMGVSGVLILWLGINALCGILSLVSLVIYTTIYTPAKRVTPFAVLIGAFPGAF
                     PPLLGWVAGKGEIGLEALILYALQFIWQFPHFWAIAWVLHDDYLKAGFRMLPGPHGRD
                     RSSAFQTMVYTLGLVPMAFLPLAFHFTGWFTALLLAACGIY"
BASE COUNT          355 a          523 c          492 g          448 t
ORIGIN      
        1 cggttcgcct ggcctttgcg gccacacggc gccgttccga ttggctgatc gcgacttcgc
       61 ccttcatgtt cctcattccg gctatcgcgt tcttcggtcg cggaagaagg aagatcatgg
      121 atgtccgcga cctcgtatgg tgctacatgc cggaccgtaa catattccag cgggccattc
      181 gctccctctt gcaacgcgtg atcgtgcggt ccctgcggaa attcgaccac atcaccgtaa
      241 ccaacagcag cgaagaacag tggatgatcc gatcggcggc tgttccgcgt gcccggctga
      301 gcatagtaag caacggactt tcgcaggatc gattccgcag actaagtaat atcgcctact
      361 atccgccgga gcacccgttg gtcattacct acatcggtaa catcggcaat ggacaggacc
      421 tggggcctgt gatcgatgtg gtgcgccatt tgccggacgt gaaattgaac ctgatcggcg
      481 atggcatcga attggaacgc taccgaaaat acatccggca ggaaaacatc cgcaacgtgc
      541 agtttttcgg aaagatgaag tggaatcgtc tgttgccgtt ctatcagact tcatccattc
      601 tcctcgcacg tctcggcaag aattatcaga gcgcgttgcc cagtaaattg ttcgagtatt
      661 tggccaccgg attaccggtc ttgttctacg gcaacgggga ggccgcccgc ttcctgaaag
      721 acttcgaaaa tgtattcatc gttcggcagg aggatcagga agaattgcgc cgcgcgatcc
      781 ggcaactgca gacgctgaca ccgccccgct cattcgctaa tatcctgcgt gtggagtcca
      841 atttcatccg ggagcacctg aacctccgct accttgattt gttaaaagat ccagcggaac
      901 gggttacggt tcttgagccc gtcgggatgg atgctcagag cagagcggat tggtcccttc
      961 gggaggcctg acgcgccttg gaacccttcc cggaattccc taaacttgca gcccttttac
     1021 aaggcatccc tgctaccgga tggcgcaaaa atcaatcgct ttatcggata aacccgtgac
     1081 cctctcggca cgcatccagg attacatcca gctcacgaag tttcggctct cttccctggt
     1141 agtattctca gcggcaacgg gttacatcat cgcggaaggc gctgactttc agtggtctac
     1201 tttattgttg ctcatgctcg gcggcttcct ggtaacgggg tcgtccaacg cgttcaacca
     1261 ggtcattgag cgtgatctgg ataaattgat ggaccgaacg gccaatcgcc cgctacccgc
     1321 cggcaggatg tccgtccccg aagcccttgt tgcttccgtt ctgatgggtg tctcgggggt
     1381 cttgatattg tggttgggaa tcaatgcctt gtgcggaatt ctcagtctgg tcagtctggt
     1441 catttatacg accatctaca cgcccgcaaa gcgggtgact ccattcgcgg tattgatcgg
     1501 cgcgttcccg ggagcttttc cgcccctgct gggctgggtg gccggtaaag gtgagatcgg
     1561 actcgaggca ctcatcctgt acgcccttca gttcatctgg cagttcccgc acttttgggc
     1621 gattgcctgg gtcttgcacg atgattacct gaaggccggc ttccgcatgt tgcccggacc
     1681 ccacgggcgc gatcgttctt cagcgtttca gaccatggtg tacacgctcg gtctggtacc
     1741 catggctttt ctgccattgg catttcactt caccggttgg ttcaccgcct tgctgctggc
     1801 agcctgtgga atctactt
//
LOCUS       DAOTON010000631         1817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_50279_length_1817_cov_3.339387, whole genome shotgun sequence.
ACCESSION   DAOTON010000631 DAOTON010000000
VERSION     DAOTON010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50279_length_1817_cov_3.339387"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..523
                     /locus_tag="P5292_11205"
     CDS             113..523
                     /locus_tag="P5292_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39738.1"
                     /translation="MIIRNAQLIVTLFVACLLVSEANAQLNQVDPDKAGEQLKTYFFV
                     MLVKGPNRTQDSATVVQMQRGHLENMDRMAKEGKLDIAGPFATDIDWRGILIFNMDSE
                     EAVRKELELDPMIQAGRLSYLIHPWMSMKGAVLR"
     gene            623..>1817
                     /locus_tag="P5292_11210"
     CDS             623..>1817
                     /locus_tag="P5292_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRS39739.1"
                     /translation="MPIWKIVTMRWKRITLILVVVSAGFLSFRAAEDYYFEVSKNLDI
                     FTTLFRDVNTYYVDSVQPGDLMKKGIDAMLRTLDPYTVFIPESDVEDYRMTHISAEYG
                     GIGALVHERDGAIEVSEVYEGFPAQKAGIQVGDHILSVNGMSTTGRKVDDVTEVMKGQ
                     KGTSVRLSLQREGVAQPFEKVIVRDEIKFKNVPYFGKISDKVGYIRLTQFLENSAAEV
                     REALVTLKQDSGLQAVVLDLRGNGGGLLKEAVDIVNLFVDKGQKIVSQKGKVREMNIE
                     YTANKTAVDTVIRVAVLVDRGSASASEIVTGAIQELDRGVVIGQRSFGKGLVQQTYPL
                     SYNSLLKVTIAKYYTPSGRCIQALDYSHRSAEGVVNKVADTLINEFRTRNGRSVFDGS
                     GVYPDV"
BASE COUNT          438 a          459 c          517 g          403 t
ORIGIN      
        1 atccggaatc gaaacgcgat tttgatctgg aggaggcatt ggcgatgtat gcctggcatt
       61 gcgaacacca tctcgcgcat atacggcaac tgaaacagcg tttacaatgg gcatgatcat
      121 tcggaacgcg caactgattg ttacattgtt cgtcgcgtgc ttactcgttt ctgaagcgaa
      181 cgcgcaactc aatcaggtag acccggataa ggccggcgag cagttgaaga cgtatttttt
      241 cgtcatgctg gtcaagggcc cgaacaggac ccaggattcg gcaacagtgg tgcaaatgca
      301 acgcggtcac ctcgagaaca tggatcgcat ggccaaagag ggtaaactcg atatcgcagg
      361 tccctttgcc acggacatcg actggcgcgg aatcctgatc ttcaatatgg attcagagga
      421 ggcggtcagg aaagaactgg aactggaccc catgatccag gccggcaggc tgagttacct
      481 gattcatccc tggatgtcca tgaagggcgc ggtcttgcgg taggatttcc cttaaccttt
      541 tttaagaacg ggaggccaac tttttcccgt tcttgcagta gaaaaccagt cggccgccga
      601 ccggcccgac aattcctttc ttatgcctat ctggaaaatc gtcaccatgc gctggaagcg
      661 gatcaccctc atcctggtcg ttgtttctgc cggcttcctt tccttccgcg cagccgaaga
      721 ctactatttc gaggtcagca agaacctcga cattttcacc acgctctttc gcgacgtgaa
      781 tacgtattac gtggatagtg tacaacccgg ggatttgatg aaaaagggga tcgacgcgat
      841 gttgcgtaca ctcgatccct atacggtttt catcccggaa agcgacgtgg aagattatcg
      901 aatgacccac atctcggctg aatacggcgg gatcggtgcg ctggtacatg aacgcgacgg
      961 tgccatcgaa gtcagtgagg tgtatgaagg tttcccggcg caaaaagccg gcatacaggt
     1021 gggcgatcat atcctctccg tcaatggcat gtcgactacc ggtcggaagg tggacgatgt
     1081 gaccgaagtg atgaagggcc agaaaggcac atcggtcagg ctttcactgc aacgcgaagg
     1141 cgtggcgcag ccgttcgaga aagtcattgt gcgcgacgag atcaagttca agaacgtgcc
     1201 ttatttcggc aagatcagtg ataaagtcgg ttacatccgg ctgacccagt tccttgaaaa
     1261 ttccgctgcc gaggtgcgcg aagccctggt cacgcttaaa caagactcgg gtttgcaggc
     1321 agtggtactt gacctgcgcg gcaatggcgg cggattgctg aaagaggcgg tggacatcgt
     1381 caacttgttc gttgacaaag gacaaaagat cgtttcccag aagggaaaag tccgggagat
     1441 gaacatcgag tatacggcca acaagactgc tgtcgataca gtcatccgcg tggcggtact
     1501 cgttgaccgc ggttccgcat cggcttcgga gatcgtgacc ggcgccattc aggaactcga
     1561 tcggggagtg gtcatcgggc aacgaagctt cggtaaggga ctggtacaac aaacgtatcc
     1621 gctgtcctac aattcgttgt tgaaagtcac cattgcgaaa tactacacgc catccggacg
     1681 ttgcatccag gcactcgact atagtcatcg aagcgcggag ggcgtcgtga acaaggtggc
     1741 ggatacgctc atcaacgaat tccgtacgcg gaatggtcgt tcggtcttcg acggcagcgg
     1801 tgtttatccg gacgtct
//
LOCUS       DAOTON010000632         1810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_50577_length_1810_cov_2.734473, whole genome shotgun sequence.
ACCESSION   DAOTON010000632 DAOTON010000000
VERSION     DAOTON010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50577_length_1810_cov_2.734473"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..824)
                     /locus_tag="P5292_11215"
     CDS             complement(90..824)
                     /locus_tag="P5292_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRS39740.1"
                     /translation="MLLSLFTAEGMIGLVTLVLLEIVLGIDNIIFIAILTGYLPKKDQ
                     QRARALGLTMAMVFRIVLLLGISWLVHLTEPLFQVGPLSPSGRDLILFAGGVFLVFKT
                     LKEIIHKLSAPELDENGKVIKEKKLTVTQAILQIALIDIVFSFDSILTAVGLSSDLQV
                     MITAVVISMIVMLLFAPYVSNFINKYPTIKMLALAFLVCIGGLLIVEAFHLHIEKSYV
                     YVAMGFSLIVEMLNIRLRQLHRGSSH"
     gene            complement(876..952)
                     /locus_tag="P5292_11220"
     tRNA            complement(876..952)
                     /locus_tag="P5292_11220"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(916..918),aa:Pro,seq:ggg)
     gene            1385..>1810
                     /locus_tag="P5292_11225"
     CDS             1385..>1810
                     /locus_tag="P5292_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39741.1"
                     /translation="MSKGLLRWMQTTILLTILAFPVQEAYASHSMGADLTYQCLGGNT
                     YRVTVSFYRDCIGINAPSAPLVTINSPSCGQSLSVTCYPRPGTGQEVTPACSSSVTTC
                     QGGTFTGIQEWVYDGIVTLPMQCSDWVFGYSLCCRNAAIT"
BASE COUNT          502 a          480 c          427 g          401 t
ORIGIN      
        1 tgaagcacat taacgcactt cacacgacca aattgtcatt aaaaaggaat gaaaagcctg
       61 aaatctcccg tttacggaaa aatcaaggct caatgagaag atccacgatg taactgccgc
      121 aggcgaatgt tgagcatctc cacaatgagc gagaatccca tggccacata cacatagctc
      181 ttctcgatat ggagatgaaa cgcctcaacg atcaagagtc caccgataca aaccaggaac
      241 gcgagggcca gcatcttgat cgtaggatac ttgttgatga agttgcttac atagggcgcg
      301 aacaacaaca tcacgatcat cgaaatcact accgcggtaa tcatgacctg caagtcactg
      361 ctcagtccaa cggctgtgag gatagaatca aaagagaaaa cgatatcgat caacgcgatc
      421 tgcagaatgg cttgcgtgac cgtcagcttc ttttccttga tcacctttcc attttcatcc
      481 aactccggtg cggaaagttt atgaatgatc tctttaagtg tcttgaacac cagaaataca
      541 ccgcctgcaa acaagatcag atctcgtccg ctcggcgaaa gcggtcctac ctggaataaa
      601 ggttccgtca ggtgcaccag ccaggagatc cccaacaaca agacgattcg aaataccatg
      661 gccatggtaa gaccaagggc gcgggcacgc tgctggtcct tcttcggcag gtagccggtg
      721 aggatggcga tgaaaatgat gttatcgata cccagcacga tctccaacag gaccagggta
      781 accagtccga tcatgccttc ggcagtgaag agggatagca gcatcagcag taaacaaatg
      841 gaataggcca gaacaaactg gcctattcct gaatctggtc ggggtggcgg gattcgaacc
      901 cacggcctcc agcaccccat gctggcgcgc taccaggcta cgctacaccc cgaaaaaccg
      961 gccgaaaacc agggttcccg gaccctttga acgtcgagca tctgacgttc tattcactgc
     1021 attttcctgc ctgcaatggg gttgcggctg aaaaatcagg tcgcaaatgt atcaaaaagt
     1081 ttgaccccta agggccggga ctggtttcgc aaaagaataa atgcggaaaa caggcccgta
     1141 ttcggaggtt tcaggcatta acgaacgatt tcttgcaacc actcttcctt cagccaatca
     1201 accgattcca gttttcaatt caaaatgaca ccattcagtc tggttaaaca tacgtttccg
     1261 gtaaaacctt acaactgact agtattcaac tcattcaaaa tacttaggga aagcgccgca
     1321 atagttttaa ggtgtgaaag caggaagcac cgtgacaggc ttcctaaact gaaaaacagg
     1381 cggcatgagc aaaggattac tccgctggat gcaaaccacg atactgctta cgatcctcgc
     1441 cttcccggtg caggaagcgt acgccagtca ttccatgggt gcagacctga cgtatcaatg
     1501 ccttggtgga aacacctatc gggttacggt ttccttttat cgggattgta tcggaatcaa
     1561 cgctccttcg gctccgttgg tcaccatcaa ttctccatcc tgtggacaaa gcctgagcgt
     1621 gacctgctat cctcgtcctg gcaccggaca ggaagtgaca ccagcctgtt cctcttctgt
     1681 taccacctgt cagggcggta cgtttaccgg aatccaggaa tgggtgtacg acggtatcgt
     1741 gacccttccg atgcaatgct cggactgggt cttcggctat tcactttgct gccggaacgc
     1801 ggcgatcacc
//
LOCUS       DAOTON010000633         1809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_50603_length_1809_cov_3.735462, whole genome shotgun sequence.
ACCESSION   DAOTON010000633 DAOTON010000000
VERSION     DAOTON010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50603_length_1809_cov_3.735462"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="P5292_11230"
     CDS             <1..510
                     /locus_tag="P5292_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRS39742.1"
                     /translation="LERTGIRPTLELNGIWGGYTGEGSKTVLPSKAFAKISMRLVPNQ
                     SSDEITKLFADHFKKIAPKSVKVEVRPHHGGEPVVTPTDSVAFKAASMAMEQTFGKKP
                     IPTRGGGSIPIVALFEKELGLKSVLMGFGLDSDLIHSPNENYGLFNFYKGIETIPLFF
                     KNFVELNGK"
     gene            583..>1809
                     /locus_tag="P5292_11235"
     CDS             583..>1809
                     /locus_tag="P5292_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRS39743.1"
                     /translation="MRNTYLAILGFLLALNLHAQPAGFSWTEKTSAGRTYRTVTNDPS
                     GTRFYTLPNGLTVILSVNKNEPRIQTLIVTKAGSKTDPSTNTGLAHYLEHMLFKGTDK
                     FGTRDWAKEEPLLNEIDRLYEVYNKTKDPAERTRIYAQIDSVSYVASGYAIANEYDKM
                     CSSMGAKGTNASTSFDRTDYINNIPANMLDKWLELEGERLRKPVLRLFHTELEAVYEE
                     KNNGLDDDDQKVFETIFANLFLKHNYGLQTTIGTIDHLKNPSLNEIRKYFYANYVPNN
                     MAVILAGDFDPDVAIAKIEKTFAFMQPKPVANYTYQAETPISQPVVREVFGQTTSKVN
                     IAFRMPGAATDDAMMLSLVSNMLNNGKAGLIDLNLVKKQQLLESYAFNWNLKDYSVLW
                     LGATPREGQSLETARDL"
BASE COUNT          474 a          541 c          437 g          357 t
ORIGIN      
        1 ctggaacgca ccggcatacg gcccaccctc gagctgaacg gtatctgggg cggctacacc
       61 ggagaaggat ccaagaccgt actgccctcg aaagccttcg caaagatttc gatgcgcctc
      121 gtccccaacc agtcgagcga cgagatcacg aaactgtttg ctgaccactt caagaagatc
      181 gcgccgaaat cggtgaaagt ggaagtccgt ccgcaccatg gcggagaacc cgtcgttacc
      241 ccgactgatt ccgtcgcgtt caaagcagcc agcatggcca tggaacagac cttcggcaag
      301 aaaccgatcc cgacacgcgg cggtggttca attccgatcg tcgccttgtt tgaaaaagaa
      361 ctcggcctca aatcggtact catgggcttc ggcctggatt ccgatctcat tcactccccg
      421 aatgaaaact acggcctgtt caatttttac aaaggcatcg aaacgattcc gctgttcttc
      481 aagaactttg ttgaattgaa cggaaaataa tcaagcagaa agccacccgt aaggtggctt
      541 tcgctttatc cgaccattaa cctactcaca cctcctacta ttatgcgcaa cacctatctc
      601 gcgatccttg gcttcctgct tgccttgaat ctacatgccc agccagcggg tttcagctgg
      661 acggaaaaaa cgtccgccgg tcgcacgtac cgtacggtca ccaacgatcc aagcggcacc
      721 cggttctata cgttaccgaa cggcctgacg gtcattctct cggtaaacaa gaacgaaccg
      781 cgcatccaga cgctgattgt caccaaagcc ggcagcaaga ccgacccgtc aacgaacacc
      841 ggtctggcgc actacctgga acacatgctc ttcaaaggca ccgacaaatt cggcaccagg
      901 gactgggcga aggaagaacc cctgctgaac gagatcgacc gcttgtatga agtctacaac
      961 aagacgaaag atcctgcaga acgcacccgc atctatgcgc agatcgactc ggtaagctac
     1021 gtcgcttccg gctacgccat cgccaacgaa tacgataaga tgtgctccag catgggcgcc
     1081 aaaggcacca atgcatccac cagcttcgat cgtaccgatt acatcaacaa catcccggcc
     1141 aacatgctgg acaaatggct ggagctcgaa ggggaacgtc tccgcaaacc ggtcctgcgc
     1201 ctgttccaca ccgaactcga agcagtctat gaggaaaaga acaacggatt ggatgatgat
     1261 gaccagaagg tctttgaaac catcttcgcc aacctctttc ttaaacacaa ctacggtctg
     1321 caaacgacga tcggcacgat cgatcatctg aagaatcctt ccctgaacga gatccggaag
     1381 tatttctatg ctaattacgt tccgaacaac atggccgtga tcctggcggg tgatttcgat
     1441 cccgatgtcg ctatcgccaa gattgaaaag acctttgcat tcatgcaacc caagcctgta
     1501 gccaactata cctatcaggc ggaaacgccg atctcccagc cggttgtccg ggaagtgttc
     1561 ggccagacta catcaaaggt caacatcgcg ttccggatgc cgggggcggc taccgacgac
     1621 gccatgatgc tgtcgctggt cagcaacatg ctgaacaacg gcaaggccgg cctcatcgac
     1681 ctgaacctgg tgaagaaaca gcagttgctc gagagttacg cattcaactg gaacctgaaa
     1741 gactacagtg tgttgtggct tggcgctacg ccgcgtgaag gccagtcgct cgaaaccgcc
     1801 cgcgatctt
//
LOCUS       DAOTON010000634         1805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_50754_length_1805_cov_3.075429, whole genome shotgun sequence.
ACCESSION   DAOTON010000634 DAOTON010000000
VERSION     DAOTON010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50754_length_1805_cov_3.075429"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1805)
                     /locus_tag="P5292_11240"
     CDS             complement(<1..>1805)
                     /locus_tag="P5292_11240"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRS39744.1"
                     /translation="EDKDGDLWIGTDNGLNRYNRHNGSFTRFLHDPSNARSLSNNLVS
                     GIVQDKAGILWIGTANGLNALDPATGNFTTYRSTAGNPVTLSGNTISTLMADRSGTIW
                     VGTSGSGLNAWDPVSKKAIRYLAAPGQLSENDVVALAQDRQGVVWIGTVNGGLNSLSA
                     NGKFRSYTADHNELPNNSVFSIHEDRQGVLWVGTLGGGLVAMNAARTSQRVYRHDVQN
                     AESVSGNKVWSIFEDAAGTLWFGTSNGISYYNRTIAKFVTHRPSQTDDSNNNSVFSVF
                     EDPRGICWTGVLGGGVHAFDRKENRFVDERFPFLADPRLRFSNPFSLLVDNQGTLWVG
                     TNDGLLSVDPRSGSIVQYRNAPSDPGSLPNNYVRTLYQDRAGTLWIGTHGGGLARLRQ
                     GKFDTFRNDPKNKSSIPGNVVMHLLETRDGKFWVATYGGGLAQFDRNTGTFTAFRFDI
                     RNAGSISSDFVNDLHEDAKGRLWVATYGGGLNRMESNSSFTHFTERDGLPNNVINGIL
                     EDRKGFLWVSSNNGVFKFQTEGRSLSTHTYNVQDGLQFKFNEGACFAGVGGWMYFGGS
                     SGLNAFHPDSINDNSFIPPVVITRFYLFEKPARMD"
BASE COUNT          371 a          504 c          523 g          407 t
ORIGIN      
        1 tatccatccg cgccggcttc tcgaagaggt aaaaccgggt gatcaccacc ggcggtatga
       61 aggagttgtc gttgatactg tcgggatgga aagcgttcag gccgctgctg ccgccgaagt
      121 acatccagcc tccgaccccg gcaaaacagg cgccttcgtt gaacttgaac tggagaccgt
      181 cctgtacatt gtaggtgtgc gtactcagcg aacgcccttc cgtttggaat ttgaatacgc
      241 cgttgttgga gcttacccag aggaatcctt tacggtcttc caggattcca ttgatgacat
      301 tgttgggcaa cccgtcccgc tcggtaaagt gcgtaaaact actgttgctc tccatccggt
      361 tcagaccgcc accataggtg gctacccaaa ggcgcccttt cgcatcttcg tgcaggtcgt
      421 tgacgaagtc gctgctgatg ctaccggcat tgcggatatc gaaacgaaaa gcggtgaacg
      481 tgccggtgtt ccggtcgaac tgtgccaagc ccccgccgta cgtggcgacc cagaattttc
      541 cgtcgcgcgt ttcgagcagg tgcatcacca cattccccgg gatggagctt ttgtttttag
      601 gatcgttccg aaacgtgtcg aattttccct ggcgcaagcg cgccaatccg ccgccgtggg
      661 tgccgatcca cagggtgccg gcccggtcct gatacaaggt gcgtacatag ttgttgggta
      721 acgacccggg atccgatggc gcattgcggt attggacgat cgaaccggag cgcggatcta
      781 cggaaagcaa gccgtcgttt gtcccaaccc aaagcgtccc ttggttatcg acgagcaacg
      841 aaaacgggtt agaaaaacgc aaacgcggat cggcaagaaa gggaaagcgt tcgtcgacga
      901 accggttctc cttacgatca aaggcatgaa cgcctccgcc gagtacgccc gtccagcaaa
      961 tgccgcgggg atcttcgaat accgagaata cgctgttgtt gttcgaatcg tccgtttgcg
     1021 aagggcggtg cgtaacgaac ttggcgatgg tgcggttgta atagctgatg ccgttcgacg
     1081 tgccaaacca aagcgttccg gcggcgtctt cgaagatgct ccagaccttg tttccggaaa
     1141 cggattccgc gttctgtacg tcgtgccggt agacgcgttg gctggttcgc gcggcgttca
     1201 tcgccacaag tccgccaccg agtgtgccga cccacaggac accctgccgg tcttcgtgaa
     1261 tggaaaagac gctgttgttg ggtagttcat tgtgatccgc cgtgtaggac ctgaattttc
     1321 cattcgccga aagactgttc aagcccccgt tcaccgttcc gatccagacg acaccttgcc
     1381 ggtcctgtgc cagtgcgaca acatcgtttt cgctcaactg tccgggagct gccaggtaac
     1441 gaatcgcctt cttcgaaacc ggatcccagg cgttgagtcc gctaccgctg gtgccaaccc
     1501 agatcgtacc ggaacgatca gccatcaggg ttgaaatcgt attgcccgaa agtgttacgg
     1561 ggttaccggc agtgctgcgg taggtggtga agtttccggt agcgggatcc aaggcgttca
     1621 gtccgttggc ggtgccgatc cagaggatgc ccgctttgtc ctggacgata ccggaaacaa
     1681 gattgttact cagtgaccgt gcattcgagg ggtcgtggag aaagcgggtg aacgatccat
     1741 tgtgccggtt gtatcggttc aagccgttgt cggtgccgat ccaaagatcc ccgtccttgt
     1801 cttcc
//
LOCUS       DAOTON010000635         1802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_50872_length_1802_cov_3.112192, whole genome shotgun sequence.
ACCESSION   DAOTON010000635 DAOTON010000000
VERSION     DAOTON010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50872_length_1802_cov_3.112192"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..>1802)
                     /locus_tag="P5292_11245"
     CDS             complement(162..>1802)
                     /locus_tag="P5292_11245"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619012.1"
                     /note="biotin-containing enzyme that catalyzes a two step
                     carboxylation of pyruvate to oxaloacetate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HRS39745.1"
                     /translation="LRSAIPNILLQMLIRGANAVGYTSYPDNLVEKFIEKSWENGVDV
                     FRIFDSLNWIKSMEVSIRAVRERTGGLAEACICYTGDLLDPKRSKYNLRYYLDMARRL
                     EDAGAHVLGIKDMAGLLKPYAAEVLVKELRKAVSLPIHLHTHDTSGIQSATLLKAIES
                     GVHVVDVALASLSGLTSQPNFNVLAEALRNTPHATNFNIDSLNAFSNYWETVREYYYP
                     FESGLMAGTAEVYKHEIPGGQYSNLKPQAISLGLADRMDDIKKAYEEVNLLFGDIVKV
                     TPSSKVVGDLAMFMVTNKLTKEDLFTRGETLSFPESVKGMLRGDLGQPDGGWPKELQR
                     IVLKDEQPYTDLPNAHLPPVDFEKEFETFQKQYDNYQGFSDFLSWKFYPKVFDEYYRF
                     RKQFGDVSSLPTVNFFYGMKPNEEILVDIGTGKTLLIRLLYVAAETDDNGNRAVFFRL
                     NGQTRSVEVKDRKAQVKKVTNPKASGADQIGAPLQGRLSKVFVKGGEAVKKNTPLFTI
                     EAMKMETTITAPRDLTVKQVSLSEGSMVERDDLVVSVD"
BASE COUNT          362 a          490 c          517 g          433 t
ORIGIN      
        1 gatatcattg acaaattatt tggcaacgat atcagagttc gaatgcccaa atagaaaggg
       61 gtataatgct gcgagtcggc agggatgttt agccgggaat gaccaattcg tcccaagaat
      121 agctgccggt caatgaatga tggtcccgga ttgaaccatc tttaatccac cgacaccacc
      181 aaatcatccc tctccaccat ggagccttcc gagaggctga cttgcttgac ggtcaggtcg
      241 cggggagcgg tgatggtggt ttccattttc atggcttcga tggtgaagag cggcgtgttc
      301 ttcttcaccg cttcaccgcc cttgacgaag actttcgaaa gccgtccctg caagggtgcg
      361 ccgatctggt cggctccgct cgctttggga ttcgtgactt tcttcacctg cgccttgcgg
      421 tcctttactt ccacactgcg cgtctgtccg ttcaaccgga agaacacggc gcggttgccg
      481 ttgtcgtcgg tttcggctgc tacgtagagc aagcggatga gcaacgtttt tcccgtgccg
      541 atatcgacca ggatctcttc gttcggcttc atgccgtaaa agaagttcac cgtcggcagc
      601 gaactcacgt cgccgaactg cttccggaag cggtaatact cgtcgaatac tttcggatag
      661 aatttccagg acaggaagtc tgagaatccc tggtagttgt cgtactgctt ctggaaggtc
      721 tcgaattcct tttcaaaatc caccggcggg aggtgggcat tcggaaggtc ggtgtacggc
      781 tgctcgtctt tcaggacgat ccgttgcagt tccttcggcc agccgccgtc gggctgacca
      841 aggtcgccgc gcaacatgcc tttcaccgat tcggggaacg aaagcgtttc accacgggtg
      901 aacaggtctt ctttggtcag cttgttcgtg accatgaaca tggcgagatc gcccaccacc
      961 ttcgaggaag gcgtgacctt cacaatgtcc ccgaacaaga ggttcacttc ttcgtaggct
     1021 ttcttgatat catccatccg atcggccagg ccgagcgaga tggcttgcgg cttcaggttg
     1081 gagtactggc ctcctggaat ctcgtgcttg tacacttcgg ccgttccggc catcaggcct
     1141 gattcgaaag gatagtaata ttcacggacc gtctcccaat agttggagaa ggcgttgagg
     1201 gagtcgatgt tgaaattggt cgcgtgtggc gtattgcgca gggcttcggc cagcacgttg
     1261 aagttgggct gggaggttag cccggagagc gacgccaggg cgacgtccac cacgtgtacg
     1321 cccgattcga tcgctttcag cagggtagcc gattgaatgc ccgaggtgtc gtgtgtatgc
     1381 aaatgaatcg gcaggctgac cgctttgcgg agttctttca ccagcacttc cgccgcatac
     1441 ggcttcaaca aaccggccat gtccttgatg ccaagcacgt gcgcgccggc atcttccagc
     1501 cgccgcgcca tgtcgaggta gtagcgcagg ttgtatttgc tgcgcttcgg gtccagcaga
     1561 tcgccggtgt aacagataca ggcttccgcc agtccgccgg tgcgttcgcg aacagcccgg
     1621 atgcttactt ccatgctctt gatccagttc agcgaatcga agatgcggaa gacgtcgacg
     1681 ccgttctccc agcttttttc gatgaacttc tccaccaggt tgtcggggta ggaggtatag
     1741 cccaccgcgt tcgcgccgcg gatgagcatt tggaggagaa tgttcggaat agcagaacgg
     1801 ag
//
LOCUS       DAOTON010000636         1802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_50884_length_1802_cov_2.362335, whole genome shotgun sequence.
ACCESSION   DAOTON010000636 DAOTON010000000
VERSION     DAOTON010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50884_length_1802_cov_2.362335"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="P5292_11250"
     CDS             complement(<1..1345)
                     /locus_tag="P5292_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39746.1"
                     /translation="MLMLRTCFFCLVSLLLTRSASAQQAIVTYAGNSGNETFYDVVQL
                     SDQTFLVCGYATDLNWLPVGVPTAVLPATGINNGLGTSKYGYLLHLSSDLSQILHILH
                     FPQGAVEDIRFIKTSNAPGEPTGNLYISGNTLDTEPNDGGYFIAKLDNNFVNGVPTTL
                     SWVRRVWAEGYPKDYHPWDVNGNGEIYYILGQSHAYDWAAIYCLDASGERKVVENWRT
                     HWKVAGGEWKGFPASAYPGGRDSLAYSGIVLKAWGRCDLRSWTQNDYDLTEPDGNGGW
                     KKGRWPLDVLYDGPCDPNNPVTNSPGYTGYSLESCCPVYGGSSIVVDRRSGDLYIGMN
                     MKSVGPTGSPDFEPAVIAMDPSGALRWWSRLYHEITPAGDTMVSIPDQYIDGLAIDYS
                     TASGVNTLVVATRCHGNNTENLWEGNVIAANPSAQGFQNNFTGTNGNIHISWLGKL"
BASE COUNT          456 a          482 c          436 g          428 t
ORIGIN      
        1 gcaacttacc aagccaactg atgtgaatgt ttccgttggt gccggtgaaa ttattctgga
       61 aaccctgcgc tgaaggatta gccgcaatca cattgccttc ccacaggttc tcggtgttat
      121 tgccatgaca acgcgtggcc acgaccagcg tgttcactcc cgatgcagta gagtaatcga
      181 ttgccaggcc gtcgatgtat tgatccggaa tagacaccat tgtatcaccc gccggtgtga
      241 tctcgtgata aagtcggctc caccagcgca aggctccgga tgggtccatg gcgataaccg
      301 ccggttcgaa atcaggactg ccggtcggac caaccgattt catgttcatg ccgatgtaca
      361 agtcaccgga tcgacgatct acaacaatgc tggagcctcc atataccggg cagcaggatt
      421 ccaggctgta tcccgtatag ccgggtgaat tcgttaccgg gttgtttgga tcgcatgggc
      481 catcgtacaa cacatcgagt ggccaacgac ccttcttcca tccaccgtta ccatccggtt
      541 ccgtaaggtc gtagtcgttc tgcgtccagg agcgcaggtc gcaacgtccc catgctttga
      601 gtacgatgcc actgtaggcc agcgagtctc gtcctcccgg ataggcggat gcaggaaaac
      661 ctttccactc gccaccggcg actttccaat gcgttctcca attctccact accttccgct
      721 ctccactcgc atccagacaa tagatcgccg cccaatcgta tgcatgcgat tgcccgagga
      781 tgtagtagat ttcaccgttg ccatttacat cccagggatg atagtccttc ggataacctt
      841 ccgcccacac cctgcgaacc cagctgagcg tggtgggcac tccattgaca aagttgttat
      901 ccaacttggc aatgaaatat ccgccgtcat tgggttccgt atccagtgtg tttccgctga
      961 tgtacaagtt gccggtaggc tccccgggcg cattggaggt cttgataaaa cgaatgtctt
     1021 ccaccgcacc ttggggaaaa tgaagtatat gcagaatctg cgaaaggtca gacgagaggt
     1081 ggagcaggta tccatacttc gatgtaccca gaccattgtt gattccggta gccggtagta
     1141 cggcggtggg aactcccacc ggtaaccagt tcaggtctgt tgcatagccg catacgagaa
     1201 acgtctgatc ggatagctga acgacatcgt agaatgtttc atttccggaa tttcccgcgt
     1261 aggtaacaat cgcttgctga gcggaagccg aacgcgtcag taagagggat accagacaaa
     1321 agaagcaggt gcgaagcata agcatgtgct ggcaggattt acgatggcga aatgagctca
     1381 caatttattc taagaagatc aaaattccat ctggtaacag cgattcgtcc gctgctgctt
     1441 tcactctgat atataaagcg ggcccaatca aacaaatcac ccttcctgct ggagcgttaa
     1501 gtgtatgtgg tttaaccagc aagaaacgac gctttcggaa gtacatgcca acttcgtttc
     1561 accttaacca agatagaaaa catcctttta tttagaactg tagagtagtc cgacataatc
     1621 gcaccggtgc tgacatcatt acgcagcgtc atcttcaagt tccagttaaa ctagaaactg
     1681 ctcctgtgcc gcatactctt ctattcttag ccgggaaaaa gcagagttgg ccaacgaata
     1741 accggacaaa tcaaagaacc ctttgcactc tgattccaga ccggagaaaa cattgaaacg
     1801 gt
//
LOCUS       DAOTON010000637         1798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_51033_length_1798_cov_3.358577, whole genome shotgun sequence.
ACCESSION   DAOTON010000637 DAOTON010000000
VERSION     DAOTON010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51033_length_1798_cov_3.358577"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="P5292_11255"
     CDS             <1..819
                     /locus_tag="P5292_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS39747.1"
                     /translation="PDVLDSVPVTVYPLPVVEFTPISSYGCAPVVVNFDNLIEPPLGS
                     TYAWDFGDDSTSTDPDPAHVYTEPGVYDVSLAITSDFGCENRLTVDSAVVVYAWPVAD
                     FDQSAATLSVFTPLLELFDRSDLSASWQWDYGDGTVDTGVVNPQHQYADTGTYQIRLF
                     VQSPGGCPDTAYGTVRIDQELTLYIPNAFTPNGDGKNDGFIAVGIGVLEYEMWIIDRW
                     GREIYHSTSFDQPWDGSYKGGNPTCQADVYEYVIQLRDIKDRRHKFIGHVTLVR"
     gene            complement(1001..>1798)
                     /locus_tag="P5292_11260"
     CDS             complement(1001..>1798)
                     /locus_tag="P5292_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39748.1"
                     /translation="DLPWLNTVDLTPSGYTPVAGFSQFLSQYHSAKAALWDSSTNTMH
                     SLFFGGISRYTLDTATGTLLDDPNVPFVRTISRVTRYPDGAMTEYAFPETMPVLTGSG
                     AEFFPVESLPMDEQEILRLDLLSVGETVIGHIVGGIESSQPNIFFINTGVESVASARL
                     YRVVLDKEIVSSVNDRSTPAEAMAIRVGPNPAESEFQVELDTKVQGDTKVELLDLNGK
                     VVRKIAQQPVRPGRHRFNCDTRGLAPGRYQCRARCGAIEKQTGVIVK"
BASE COUNT          358 a          533 c          535 g          372 t
ORIGIN      
        1 cccgatgtcc tcgactctgt accggtcacc gtctatccgc tgccggtggt cgagttcacg
       61 ccgatcagtt cttacggatg tgcgccggtg gttgtcaact tcgacaacct catcgagccg
      121 cccttgggca gtacctacgc ttgggacttc ggcgacgaca gcacctccac cgatccggac
      181 cccgcgcacg tgtacaccga gccgggtgtg tacgatgtat cgctcgccat aacgagcgat
      241 ttcggttgtg agaatcgcct cacggtcgat agcgcggtgg tcgtttatgc atggccggtg
      301 gcggactttg accagtcggc cgcgacgctc tccgtcttta cgcccttgct ggagttgttc
      361 gaccgcagcg atctgtcggc ttcctggcag tgggactacg gcgacggtac cgtggatacc
      421 ggcgtggtga atccgcaaca tcaatacgcc gataccggca cgtaccagat ccgcctgttc
      481 gtgcaaagtc cgggcggctg tccggatacc gcctacggca ccgtacgcat cgaccaggaa
      541 ctcacgctgt acatcccgaa cgccttcacg ccgaacggcg acgggaagaa cgacggcttc
      601 atcgcggtgg ggatcggtgt gttggaatac gaaatgtgga tcatcgaccg ctggggccgc
      661 gagatctatc actcgacctc gttcgaccag ccctgggacg gctcctacaa aggcggcaac
      721 cccacctgtc aggccgacgt ctacgaatac gtcatccagc tacgcgacat caaggaccgg
      781 cgacataagt ttatcggaca tgtgacgctg gttcggtaga agggaaaagg aggaacgaga
      841 ccgtgaccga ggaacgagga aggttgaggc gaggctagcc tgctgaagct ttagcaaagg
      901 caggtacgag gaggttccgt gttccgtgtt ttggtaacgc tacctttttc gcagccactt
      961 cgggttttac attcttagcc tgacgctaag catatcctcc ttacttcaca atcaccccgg
     1021 tctgcttctc aatcgctccg caacgggcgc ggcattggta gcgaccgggt gcgagaccgc
     1081 gtgtgtcgca gttgaagcgg tgacggcccg gacgaacagg ctgctgcgcg atctttcgga
     1141 ccacttttcc gttcaggtcg agcagctcga ctttcgtgtc gccttgcact ttcgtatcaa
     1201 gctccacctg gaactcgctc tccgcgggat tcggcccgac gcggatcgcc atggcttcgg
     1261 ccggtgtcga gcgatcgttg acggaagaga cgatctcctt gtcgagcacg acccggtaca
     1321 aacgggcaga cgcgacgctt tccaccccgg tgttgatgaa gaagatgttc ggctgtgaac
     1381 tttcaatacc gcccacgatg tgaccgatca cggtttctcc gaccgataac agatccagcc
     1441 gcaggatctc ctgttcatcc atcggaagac tttcgacggg gaagaattcc gcgccactgc
     1501 cggtgagcac cggcatcgtt tccggaaacg cgtactcggt catcgctcca tcgggataac
     1561 gcgtgacccg gctgatggtc cgaacgaacg gaacattcgg gtcgtccagc aaggtgccgg
     1621 tcgcggtgtc cagggtgtaa cggctgatgc ctccgaagaa cagggagtgc atggtgttgg
     1681 tggacgaatc ccaaagcgct gctttcgcgc tgtggtattg actcaggaac tgggaaaatc
     1741 ccgccaccgg cgtataaccg gagggcgtca gatcgaccgt gttcaaccag ggaagatc
//
LOCUS       DAOTON010000638         1793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_51231_length_1793_cov_3.080552, whole genome shotgun sequence.
ACCESSION   DAOTON010000638 DAOTON010000000
VERSION     DAOTON010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51231_length_1793_cov_3.080552"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1341)
                     /locus_tag="P5292_11265"
     CDS             complement(160..1341)
                     /locus_tag="P5292_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39749.1"
                     /translation="MKSGEATVDLRDALDRLVAYVEREGYKGYDPYDTLNAVIPFRWF
                     GRWGEVIATQIQKRNPVNIRPLLGIRKGHNPKAIGLFLHAYALLQRKFPERDYQKQMS
                     FLLNWLSENRSKGFQHACWGYNFGWSTPKKHLPAFAPTVVATGFVVKGLWEYHQLTGD
                     SLAKQLILDAGKFVVNDLPRTELKNGISFSYSPYFPDVCYNASLLGAEILARAYALNG
                     DETDKRLAVAATEFVVGQQHADGHWKYSFEPDSGVERHQVDFHQGYVIDSIAYVMQLT
                     GHHPAHWPEAVRKGLRYFHDVQFHADGRGLWRIPKEYPTEIHNQSQGILTLNRWRALS
                     PETAPRAKTIADWTIRHMQDHKTGHFYYRQYPHHTNRISFMRWSNAWMMLALAELVNS
                     E"
     gene            complement(1338..>1793)
                     /locus_tag="P5292_11270"
     CDS             complement(1338..>1793)
                     /locus_tag="P5292_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRS39750.1"
                     /translation="IVGEVCHFVDLCSFLAASPVTQVSAMTMADPANQQDTLSITLGF
                     ANGSVAGITYFSNGNKQLPKEYLEVFQSGNVSVMNDFRELSSFGKNSVSEKGVQDKGH
                     KAEVRAFLEAVRNGQPSPIPASDVFNATAATFAILESIATKGQVVRLDQ"
BASE COUNT          379 a          494 c          495 g          425 t
ORIGIN      
        1 ccggaaataa cttttaacca accctgtctg tcaggtgaag ttctaactat tacaagtcag
       61 ccatcgaaac tcactattca ctattcacta ttcgctgttc gctattcgct attcgctatt
      121 cgctagcacc ttcgctaaag ctacggcggg cagggttcac tactcactat tcaccaattc
      181 cgccaatgcc agcatcatcc aggcgttgct ccaacgcatg aaggagatgc ggttggtatg
      241 gtgggggtat tgtcgataat agaaatgtcc cgtcttatgg tcctgcatgt ggcggatggt
      301 ccagtcggcg attgttttgg ctctgggggc cgtctccggc gatagtgcgc gccagcggtt
      361 cagcgtcagg atgccctggc tctggttgtg gatctcggtg ggatactctt tcggaatgcg
      421 ccacagtccg cgcccgtcgg catgaaattg cacatcgtgg aaatagcgca gacctttgcg
      481 cacggcttcg ggccaatgcg cgggatggtg cccggtcaat tgcatcacat acgcgatcga
      541 atcgatcaca tagccctggt gaaagtcgac ctggtggcgt tccacacccg aatcgggttc
      601 aaaactgtat ttccagtgtc cgtcggcgtg ttgttgtccg actacgaatt cggtggcggc
      661 aactgccagg cgcttatccg tttcgtcacc gtttaacgcg taagcacgcg cgaggatctc
      721 ggcgccgagc aggctggcat tgtagcagac atccgggaaa tagggactgt aactgaagct
      781 gatcccgttc ttcagttccg tacgcggaag atcgttgacc acgaacttcc ccgcatcgag
      841 gatcagttgt ttagcaaggg aatctccggt aagttgatga tactcccaaa gccccttgac
      901 cacgaaaccg gtggccacca ccgtcggggc gaaagccggc aggtgctttt tcggcgtact
      961 ccagccgaag ttgtaacccc agcaagcatg ttggaaaccc ttgctcctgt tttcgctcag
     1021 ccaattcagc aggaagctca tctgcttctg gtaatcgcgc tccggaaatt tccgttgcaa
     1081 caacgcgtag gcatgcagga agaggcctat cgccttcgga ttatgtcctt tccgaatgcc
     1141 tagcagcggc cggatgttga cgggatttcg cttttggatc tgcgtagcga tcacttcacc
     1201 ccatcggccg aaccagcgga aggggatgac cgcgttgagc gtatcgtacg ggtcatagcc
     1261 tttgtagcct tcgcgctcga cgtaggcgac gagacgatcc agggcgtccc gaagatcaac
     1321 cgttgcttcc ccggatttca ttgatcaagg cgcacgacct ggccctttgt cgcgatcgat
     1381 tcgaggatag caaacgtggc ggcagtcgcg ttgaaaacgt ctgacgcggg aatcggcgaa
     1441 ggttgaccgt ttcgaacagc ctccagaaaa gcgcgcactt ccgctttgtg gcccttatcc
     1501 tggacacctt tttcgctcac gctgttcttt ccaaaactcg aaagctcccg gaagtcgttc
     1561 atcacggaaa cattccccga ctggaacacc tccaggtatt ctttcggcag ttgtttgttg
     1621 ccgttcgaga aataggtgat accggctacg ctgccattgg caaagccgag ggtgattgaa
     1681 agtgtatcct gctgattggc gggatcggcc atggtcatgg ctgaaacctg ggtaaccggc
     1741 gacgcggcca ggaacgaaca gaggtccacg aagtggcaga cttctccgac gat
//
LOCUS       DAOTON010000639         1790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_51351_length_1790_cov_3.944669, whole genome shotgun sequence.
ACCESSION   DAOTON010000639 DAOTON010000000
VERSION     DAOTON010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51351_length_1790_cov_3.944669"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1346)
                     /gene="rodA"
                     /locus_tag="P5292_11275"
     CDS             complement(84..1346)
                     /gene="rodA"
                     /locus_tag="P5292_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRS39751.1"
                     /translation="MREQRSIWQNLDWVLVGLYLLLMFMGWANIYAAVYNEEHTSILD
                     LSQSYGRQAIWIGGSVLLALMILVIDGKFYAAFSYPIFGVMLLLCLSTFVLARDVKGS
                     YGWIDIGSFKLQPAEFAKFATNMALAKYLSTLGIRIQDMRTKVVSAIIIGVPTLIILL
                     QNDTGSALVFGSFIFVLYREGLSGNVLLLGFLLVVLFILSLLVDKMILFSVIGAIALV
                     FFFLVRKTRRNIAVILTGAIMAAGIVYSVDHVYKHVLQEHQRTRIDVLLGKETDLKGA
                     GYNVNQSKIAIGSGGFLGKGFLQGTQTKYDFVPEQSTDFIFCTVGEEWGFVGSLIVIA
                     LFMFLFVRIIQVAERQRSQYSRIYGYGVASIMFFHLMVNIGMTIGLAPVIGIPLPFFS
                     YGGSSLWSFTILLFVFIKLDAYRLQILR"
     gene            complement(1349..>1790)
                     /locus_tag="P5292_11280"
     CDS             complement(1349..>1790)
                     /locus_tag="P5292_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRS39752.1"
                     /translation="GSDAQYFPVVIDGMENVVEAGTAAASKVKGISICGKTGTAQNPH
                     GKDHSVFVAFAPKDNPKIAIAVTVENAGWGGTWAAPIATLMIEEYLRDSISRPDLDKR
                     MREGVILPEGAQFRPQPKDTSKQQPKTPPVTAVKVRREAVLKEH"
BASE COUNT          448 a          503 c          477 g          362 t
ORIGIN      
        1 cacagcctgc ggagtgtcgg cggattgtca gcttattgcc tgaagatgaa gatgttaaaa
       61 cgcgattgta cgctaaaaac gaactaacgc aggatctgaa gccgataggc atccagctta
      121 atgaagacaa aaaggaggat cgtgaacgac cagagcgaag aacctccgta gctgaaaaag
      181 ggtagcggaa taccgataac cggtgccagt ccgatcgtca tgccaatgtt caccataagg
      241 tgaaagaaca tgatggacgc gacaccatag ccgtaaatcc ggctgtattg ggagcgttgt
      301 ctttcagcga cctgaatgat gcgtacgaac aggaacatga atagcgcgat cacaatcagg
      361 ctgcctacga agccccactc ctcccctacg gtgcagaaga taaagtcagt actctgctcc
      421 ggaacgaagt catacttcgt ttgcgttccc tgtagaaacc ctttgcccaa aaaaccaccg
      481 gaaccgatcg cgatcttact ctggttgaca ttgtaacccg cccccttcaa gtcggtttcc
      541 ttacccaaca acacatcgat gcgggtgcgc tggtgttcct gcaaaacgtg cttgtacacg
      601 tggtcgacgc tatagacaat gcccgccgcc atgatcgcgc cggtgaggat caccgcgatg
      661 ttacggcgcg tcttgcggac cagaaaaaag aacacgaggg ctatggctcc gatcacgctg
      721 aacagaatca tcttgtcgac caacagggat aagatgaaca ggacaaccag caggaagccc
      781 agcagcagta cattcccgga gagtccttcg cgatacagga caaagatgaa ggatccaaag
      841 accagcgctg acccggtatc attttgcaac aggatgatga gtgtcggcac cccgatgatg
      901 atcgcggaaa ccaccttggt acgcatatcc tgtatccgga taccgagcgt gctcaggtac
      961 ttcgccaacg ccatattggt ggcgaacttg gcgaattcag ccggctgcag tttaaaggac
     1021 ccgatatcga tccagccgta agaacctttc acgtcgcgcg caaggacgaa cgtggagagg
     1081 cacaataaca gcatcacgcc gaagatggga tacgagaagg ccgcgtagaa tttaccgtcg
     1141 atcaccagga tcatcaacgc cagcaagacg gaaccaccga tccagatggc ctggcgaccg
     1201 taactctggg aaaggtcgag gatactggta tgctcctcgt tgtaaacggc ggcatagatg
     1261 ttcgcccagc ccatgaacat caggagcagg tagagcccga cgagcaccca atcgaggttt
     1321 tgccatatgc ttcgttgctc acgcatgatc agtgctcttt caggaccgcc tccctgcgga
     1381 ccttcacggc ggtaaccggg ggtgtcttgg gttgttgctt actggtgtcc ttgggttgcg
     1441 gacggaattg cgcgccctcc ggcaggatca cgccttcccg catgcgcttg tccagatcgg
     1501 gacgggagat ggaatcccga agatactctt cgatcatcag tgtcgcgatc ggcgccgccc
     1561 aggtgccgcc ccacccggca ttctccactg taaccgcgat tgcgatcttc ggattatcct
     1621 tcggcgcgaa agcgacgaat accgagtggt ccttgccgtg agggttctgt gccgtaccgg
     1681 tttttccgca gatgctgatg cccttcacct ttgaagcggc agccgtaccg gcttccacca
     1741 cgttctccat gccatcaatc accacgggaa aatactgggc gtcacttccc
//
LOCUS       DAOTON010000640         1785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_51568_length_1785_cov_2.763006, whole genome shotgun sequence.
ACCESSION   DAOTON010000640 DAOTON010000000
VERSION     DAOTON010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51568_length_1785_cov_2.763006"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="P5292_11285"
     CDS             <1..1021
                     /locus_tag="P5292_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39753.1"
                     /translation="SQCAGIGDTVVFAGYSYGNGSSLAQPWIVKALSDGSIASTRILP
                     IGEECFVNKVKVLSDGKILLGGYYGTGPNNPDDAWLGLCDASLDTIWNRRIDYGHAER
                     ICGLDQFPNGNYLFAGRLTLSTNGFDQNMIGRMDNAGSLAWTNIAGGDSTYDGFDDVF
                     IRNDTLFCGATTATFGQGARDFQLITFDGAGNFLIGKTYGGPEGDQVRSCIPGEFGGA
                     ILTGYSNSFPVDQQTLFVVRTDSALESTNNTVIGVSELSKNNLMVYPNPVRSNQELTV
                     SGAVPLQEIVLTDLSGRIVLRQFPNRKEINLAITSQSAGIYLLGCRYQDNDWMIRKVM
                     ISGDH"
     gene            complement(1015..>1785)
                     /locus_tag="P5292_11290"
     CDS             complement(1015..>1785)
                     /locus_tag="P5292_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS39754.1"
                     /translation="INTGADENSLLVSPDGKLGFFASDRAGGYGQLDLYQFELPESMR
                     PVPVTYMKGKVYDADTKKPLAAGFELIDLATRKTVVSSTSNVGSGEYLVCLPSGKSYA
                     LNVAKDGYLFYSETFRLDDPKAASDPLVKDIPLKPIRVGESVVLKNIFFEFDKFDLKD
                     ESRAELSKVVAFLQKNSKVRVEIGGHTDNVGSKTYNLNLSDKRAKAVYDYLVGQGIPA
                     SRMSSKGYGDAKPIADNASEQGRAQNRRTEFTIVAVDQ"
BASE COUNT          398 a          481 c          466 g          440 t
ORIGIN      
        1 ttcacagtgc gccgggatcg gagatacggt cgtattcgcc ggctattcct atgggaatgg
       61 atcctcgttg gcacaaccct ggatcgtaaa ggcattatcg gatggatcca tcgcctcaac
      121 gcgcatccta cccatcggcg aagagtgttt tgtgaataaa gtgaaagtcc tgtccgacgg
      181 aaagattctc ttggggggtt actacggtac cgggccgaac aacccggatg atgcctggct
      241 tggcttgtgc gacgcctcgc tggatacgat ctggaacagg agaatcgact acggacatgc
      301 cgaacggatc tgtggcctcg accagttccc caatgggaac tacctctttg ccggccggtt
      361 gacccttagc accaatggct ttgatcagaa catgatcggc cggatggata acgctggctc
      421 ccttgcctgg acgaacattg ccggtggcga ttccacctac gacggttttg atgatgtatt
      481 catccgaaac gacaccctgt tttgcggtgc cactaccgca accttcggtc agggtgctcg
      541 cgattttcaa ttgatcacct tcgacggtgc cggtaacttc ctgatcggaa aaacttatgg
      601 tggtcccgaa ggggatcagg tgcgaagctg tattcccggc gaatttggtg gcgcaatcct
      661 gaccggttac agcaattcct ttccggttga ccagcagacg ctttttgtcg tgcgtaccga
      721 ttccgctttg gaaagcacga acaacacggt aattggcgta tcggaattat cgaaaaacaa
      781 cttgatggtt tatccgaatc ccgtccgttc caatcaggaa ttgacagttt ccggcgctgt
      841 cccgcttcag gagatcgtac tgacggatct ttccggacgg attgtcctac gccagttccc
      901 gaacaggaag gaaatcaacc tggcgattac cagtcagtct gccggcatct atctgcttgg
      961 ctgtcgctac caggataacg actggatgat ccgaaaagtg atgatcagcg gggatcattg
     1021 atccacggca acgattgtga actcggtgcg acggttctgt gcacgcccct gttccgaggc
     1081 attgtcggcg atcggcttgg catccccgta tccctttgaa ctcatgcggg aagcaggaat
     1141 cccctgtcca accagatagt cgtatacggc ctttgcccgc ttgtctgaca gattgaggtt
     1201 ataggtctta ctcccgacat tgtcggtatg tccgccgatc tcaacccgca ctttgctgtt
     1261 cttttgaaga aaggccacca ctttcgacaa ttccgctcgt gactcgtctt tgagatcgaa
     1321 tttgtcgaac tcgaaaaaga tgttcttcaa aacgacactt tcaccaacac ggatcggttt
     1381 caacggaata tcctttacca ggggatcgga tgccgccttg ggatcatcga gtcggaatgt
     1441 ttccgaatag aacagataac catccttggc aacgttgagc gcgtagctct tacccgaagg
     1501 cagacacacc aggtactcac cgcttccgac gttcgatgtc gagctgacga ccgtcttacg
     1561 cgtcgcgagg tcgatcaact caaagccggc cgccagtggt ttcttcgtgt cagcatcgta
     1621 gacttttcct ttcatgtagg taacgggtac cggtcgcatc gattccggaa gttcgaattg
     1681 atacaggtca agttgtccgt aaccacctgc cctgtccgag gcgaaaaaac ccagcttacc
     1741 atcggggctg acgaggagtg aattctcatc ggcgccggta ttgat
//
LOCUS       DAOTON010000641         1782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_51690_length_1782_cov_2.707006, whole genome shotgun sequence.
ACCESSION   DAOTON010000641 DAOTON010000000
VERSION     DAOTON010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51690_length_1782_cov_2.707006"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="P5292_11295"
     CDS             <1..1165
                     /locus_tag="P5292_11295"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRS39755.1"
                     /translation="YGQQVELNRIHVICEQLERSSDYKIRRETVQAFNERNEFLKRGI
                     AMTPVKFGISFTTTFLNQAGALVNIYTDGTLLVNHGGTEMGQGLNTKIAQIAAAELGV
                     SLERVKVNATNTSKVPNTSATAASAGTDLNGMAVKHAVDQLKQRLCKFAAEEFTRRNP
                     DLPVSAANVRIEADVVFDERYPEKHRMSFSETTGLAHLHQVGLSSTGFYRTPDIGWDK
                     QKGWGKPFNYFAFGMAVSEVEVDTLTGYVRHLRTDILHDVGESINARIDIGQVEGAYI
                     QGLGWCTTEEVKHDANGNLLNHSPDTYKIPAFGDMPEDFRVQLLQGYPNPRAIRRSKA
                     VAEPPFMLAFATWLAIKDAVSSVGHHRQEPEFSLPATNEVILLSIEKLRPAGR"
     gene            1204..>1782
                     /locus_tag="P5292_11300"
     CDS             1204..>1782
                     /locus_tag="P5292_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRS39756.1"
                     /translation="MQQTETPDPRKDELEEDLNTIRSWRNVLVGAAILLPLGYLLWQG
                     AGTLGWQPHLLDWLHLIVRWAHVVLGVAWIGASFYFIFLENSLNRTKDLRDELAGNLW
                     AIHGGGFYYVEKYKNAPQKLPEKLHWFKYEAYFTWLTGMLLLTIVYYFNAKAFMVDPA
                     VRDISPLQAIGIGIGSLFAGWFIYDALCKSPLL"
BASE COUNT          423 a          551 c          472 g          336 t
ORIGIN      
        1 ctacggccag caggttgaac tcaaccgcat ccacgtcatc tgcgagcagc tcgaacgttc
       61 atcggattat aagatcagaa gggagacggt ccaggccttc aacgaacgca acgagttcct
      121 gaaacgaggc atcgccatga ccccggtcaa attcggcatc tcgttcacca ccaccttcct
      181 caaccaggca ggagcactcg tcaacatcta caccgacggc accctgttgg tcaaccacgg
      241 cggaacggag atgggccagg gcctcaacac caagatcgcc cagatcgccg cggccgaact
      301 gggcgtgagc ctggagcggg tgaaagtaaa cgcgacaaac acatcgaagg tgcccaacac
      361 gtcggctacc gccgcctcgg ccggcaccga cctgaacggt atggcggtaa agcacgcggt
      421 cgaccaactc aaacaacggc tctgtaaatt cgccgcggaa gagtttaccc gcagaaaccc
      481 ggacctgccc gtctccgcag ccaacgtgcg catcgaggcg gatgtcgttt tcgatgaacg
      541 ctatcccgaa aaacaccgca tgagtttttc ggaaacgacc ggccttgccc accttcacca
      601 ggttggcctc tcctcgaccg gtttctaccg caccccggat atcggctggg acaagcaaaa
      661 aggctggggg aagccgttca attacttcgc cttcggtatg gcagtcagcg aagtggaagt
      721 cgataccctg actggatacg tacgccacct tcgtactgat attctacacg acgtcggtga
      781 atcgatcaac gcgcgcatcg acatcggcca ggtggaaggc gcctacatcc agggcctcgg
      841 ctggtgtacg acggaggaag tcaagcacga cgcaaacggc aatctgctca accactcccc
      901 cgacacttac aagatcccag cgttcggcga tatgccggaa gattttcggg tgcagttgct
      961 gcaaggctat ccaaaccccc gtgccatccg acgtagcaaa gcggtggccg aacctccgtt
     1021 catgctggcc tttgcaacct ggctggcgat caaagacgcc gtctcgtcgg tcgggcatca
     1081 tcgccaggaa ccggaattca gcttaccggc gaccaacgag gttatcctgc tttccattga
     1141 aaaacttcgg ccggcagggc ggtaagccgt atagacggga agcgtaactt tcggctgcag
     1201 attatgcaac agaccgaaac acccgatccg cgtaaagacg aactggaaga ggacctgaac
     1261 acgatccgct cctggcgaaa tgtgctggtc ggagcagcca tcctcctgcc gctgggatac
     1321 ctgctgtggc aaggtgccgg cacactgggc tggcaacccc atctcctcga ctggctgcac
     1381 ctcatcgttc gctgggcaca cgtagtactc ggagtagcct ggatcggcgc cagcttctat
     1441 ttcatcttcc tcgaaaacag tctcaaccgc acgaaggact tgcgcgatga actcgccggc
     1501 aacctctggg ccattcacgg tgggggattc tactatgtag agaagtataa aaacgccccc
     1561 caaaaactgc cggaaaaact ccactggttc aagtatgaag cctatttcac ctggctgacc
     1621 ggtatgctgc tgctgacgat cgtgtactac ttcaacgcca aggcattcat ggtcgaccct
     1681 gcggtacgcg acatcagtcc cctgcaagca atcggcatag gaattggatc cctctttgca
     1741 ggctggttca tctacgacgc gctgtgcaag tcacctttac tg
//
LOCUS       DAOTON010000642         1776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_51927_length_1776_cov_3.184195, whole genome shotgun sequence.
ACCESSION   DAOTON010000642 DAOTON010000000
VERSION     DAOTON010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51927_length_1776_cov_3.184195"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="P5292_11305"
     CDS             <1..1204
                     /locus_tag="P5292_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leukotriene A4 hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HRS39757.1"
                     /translation="ALAVGDFDYHRYDDRSGVYAEPATLPMAADEFTDLPKMITAAEQ
                     LYGPYAWGRYDLIILPPSFPFGGMENPRVTFATPTIIAGDKSLTTLVAHELAHSWSGN
                     LVTNATWNDFWLNEGFTVYFENRIMEALYGKDFADMQRRLGQDDLLETVKDLGASNPD
                     TRLKLDLKGRDPDEGVSDIAYEKGNNLLLVIEQYTGREKWDRFLNDYFRKHAFSTMNT
                     EAFLEELRAGLFANDPNGWDSLKIEAWVYQPGLPANMLRIESERFSKVEKVIADWKGG
                     VAPTTEATNGWSAFEWVHFLRSLPDSLPHDDLRYLDQTFGFSRTGNNEILFSWMEYCI
                     RDRYTAIYPALESFLNEVGRRKYVKPLFQALLQTPEGKIEAKKIYAAARPNYHFITQA
                     TVDELMAE"
BASE COUNT          429 a          541 c          473 g          333 t
ORIGIN      
        1 cgcgctggcg gtcggtgatt tcgactacca ccgctacgac gatcgttccg gcgtctacgc
       61 cgagcccgcc accctgccga tggccgccga cgaattcacc gacctgccga agatgatcac
      121 ggccgccgag caattgtacg gaccgtatgc ctggggacgt tacgacctca tcatcctgcc
      181 gccctccttc cccttcggcg gcatggaaaa tccgcgcgtc acctttgcca cgccgaccat
      241 catcgccggc gataagtccc tcaccacgct ggtggcgcac gagctggccc actcgtggag
      301 cggcaacctg gtgacgaacg cgacctggaa cgacttctgg ctcaacgaag gcttcacggt
      361 gtatttcgag aaccggatca tggaagcctt gtacggaaaa gatttcgccg acatgcaacg
      421 acggctcggt caagacgacc tgctcgaaac cgtcaaagac ctcggcgcat ccaatccgga
      481 tacccgactc aaactggatc tcaaaggccg cgaccccgac gaaggcgtga gcgatatcgc
      541 ctatgaaaaa ggcaacaacc tcctgctggt gatcgaacaa tacaccggcc gggaaaagtg
      601 ggaccgcttc ctcaacgatt acttccgcaa acacgcgttc agcacgatga acaccgaggc
      661 attcctcgaa gaactgcgcg cgggcttgtt tgccaatgat cccaacggct gggactcgct
      721 gaagatcgag gcatgggttt accagcccgg cctgccggcg aatatgctgc gcatagaatc
      781 ggaacgcttc agcaaagttg aaaaagtgat agccgactgg aaaggcggcg ttgcgccgac
      841 aacggaagcc accaacggct ggagcgcgtt cgaatgggtg cacttcctcc gctccctgcc
      901 cgacagtctt ccgcacgacg atctacgcta cctcgaccag acgttcgggt tctcccggac
      961 gggcaacaac gagatcctgt tcagctggat ggaatactgc atccgtgatc gctacaccgc
     1021 aatctatccg gcactggaat ctttcctcaa cgaagtcggc cgcaggaaat acgtcaagcc
     1081 actcttccag gcactgctgc aaactcccga aggaaagatc gaagcaaaaa aaatctacgc
     1141 cgccgcccga ccgaattatc atttcattac acaggcaacg gtggatgagt tgatggcgga
     1201 atgagggacg aggggcgtgg gacgagggac gaggtgcgtg gtgcgtggtg cgaggagcga
     1261 ggagcgagga gcgaggagcg aggagcaaat tgatacaggc cgaacattcc tgcaaactat
     1321 tcagcgaagg caatcaggat ttggggagca caacacgcac acgctactcg ccagcggtcc
     1381 cgtgttctct cgtcatgtaa acgttcgtcc gcttatcagc cagatacgtg acgagatcac
     1441 ccggggctag accggaacgg tcttcttcgc ctcatcgggg acccatcaac aaatctttca
     1501 cacatcaaac ccaatcccac caggataatg tgacccctac ggggtcatcg attcatataa
     1561 ccaaggtatt tcacaaattg agattggtct gtcttccagg atcaggcatg gttcactccc
     1621 aacttcgcta aagctccggc gggcaggatt cactcccgcc tacgctaaag ctacggcggg
     1681 caggattcac tgttcactgt tcaccaaaaa actattcgct gttcgctatt cgctcctgcc
     1741 tacgctaaag cttcggcagg taaaattcgc tcccgc
//
LOCUS       DAOTON010000643         1776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_51933_length_1776_cov_2.801278, whole genome shotgun sequence.
ACCESSION   DAOTON010000643 DAOTON010000000
VERSION     DAOTON010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51933_length_1776_cov_2.801278"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="P5292_11310"
     CDS             complement(<1..642)
                     /locus_tag="P5292_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRS39758.1"
                     /translation="MKLLLRICIGLFLLLFAAGLFVFFYLRWNRPQYDGELHLQGLQQ
                     TVTTRFDANGIPHIYGANEHDVFLALGYLHAQDRLFQMELLRRVGSGRLAEIFGAQVL
                     EVDRFFRMLGFAQHADSAAQAFRNGPEAAWKQAARAYLQGINQYIELGKTPLEFRLVG
                     IPKAPFTERDLFLIVGYMTFNFEMGFRTDPLMSRLQQQLGEPYLRDLSLGYLPG"
     gene            complement(670..1548)
                     /gene="era"
                     /locus_tag="P5292_11315"
     CDS             complement(670..1548)
                     /gene="era"
                     /locus_tag="P5292_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRS39759.1"
                     /translation="MAHKAGFVSIIGKPNVGKSTLMNRILGQHLSIVSAKAQTTRHRI
                     KGILNAEDYQIVFSDTPGLLDPHYLLHQKMMDFVDAALEDADAVLFITDLAEQYMDEA
                     LVARLKKIKVPVVVVINKVDQSGIDEINKLVHGWKKHLQPHAVIPASAINDFNTTVVR
                     DTLLEVLPESPAFFPKDDVSDASLRFFTAEFIREKIFMHYRDEIPYASTVDIEAFKEG
                     EQQVHISAVIYVERDSQKGILIGKKGSSIKRVGTEARHDIESFLGKKVFLELFVKVEK
                     DWRKHENKLRRFGYSS"
BASE COUNT          358 a          516 c          536 g          366 t
ORIGIN      
        1 tccgggcaaa tagccgaggc tcagatcgcg caggtacggt tcgcccagtt gttgctgcag
       61 ccggctcatc agcggatcgg tccggaagcc catctcgaag ttgaaggtca tgtacccgac
      121 gatcaaaaac agatcgcgct cagtgaaggg cgctttcggg atgcccacga ggcggaactc
      181 cagtggcgtc ttgccgagtt cgatgtattg gttgatgccc tgcaggtagg cgcgggcggc
      241 ttgcttccag gcggcctcgg gtccgttgcg aaaagcctgc gcggccgagt cggcgtgctg
      301 cgcgaaaccg agcatccgga aaaaccggtc cacctcgagt acctgcgccc cgaagatctc
      361 cgccagccgt ccgctgccca cacggcgcaa cagctccatc tggaagagcc ggtcctgcgc
      421 gtgcaggtaa cccagggcga ggaacacgtc gtgttcgttc gctccgtaaa tgtgcgggat
      481 gccgttcgcg tcgaagcgcg tggtgacggt ttgctgcagg ccctgcaagt ggagttcccc
      541 gtcatattgc ggacggttcc agcgcaggta aaagaagacg aacaatccgg ccgcgaagag
      601 caagaggaag aggccgatac agatgcggag cagcagtttc atgggcaggc gccgctgtgc
      661 ggcgaacggt caggacgaat atccgaaccg gcgcagcttg ttctcgtgct tgcgccagtc
      721 tttttccact tttacgaaga gctcgaggaa caccttcttc ccaaggaacg attcgatgtc
      781 gtgacgggcc tccgtaccca cccgtttgat cgagctgcct ttcttcccga tgaggatgcc
      841 cttctgcgag tcgcgttcca catagatcac cgccgagatg tgcacctgct gttcgccttc
      901 cttgaacgct tcgatgtcca cggtggatgc gtaggggatc tcgtcccggt agtgcatgaa
      961 gatcttttcc cggatgaact cggccgtgaa aaaccgcagc gaggcgtcgc tcacatcgtc
     1021 cttggggaaa aaggccggcg attccggcaa tacctccagc agggtgtcgc gcaccaccgt
     1081 cgtgttgaaa tcgttgatgg ccgaggcggg tatgacggca tgcggctgca ggtgtttctt
     1141 ccagccgtgc accagcttgt tgatctcgtc gatccccgac tggtccacct tgttgatcac
     1201 caccaccacg ggaaccttga tcttcttcag gcgcgcgacc agggcctcgt ccatgtactg
     1261 ctcggccagg tcggtgatga acagcaccgc gtcggcatcc tccagcgcgg cgtccacgaa
     1321 gtccatcatc ttctggtgca gcaggtagtg cgggtccagc aggccgggcg tatcggaaaa
     1381 cacgatctgg taatcctccg cgttcagaat gcccttgatg cggtgccgcg tcgtctgcgc
     1441 cttcgcactc acgatcgaca gatgctgtcc cagaatacgg ttcatgagcg tggacttccc
     1501 cacgttcggc ttcccgatga tgctcacaaa acccgcctta tgcgccatga ggcgaaggta
     1561 gggaatcgga aggggaggag gaaatgagaa aaagttgcct caaaggtcgt agcacaagga
     1621 tagcgcctcg gttagctgaa gcatccgcgt gatccgcgtg ccatcaattt gtgaccactt
     1681 tcgctaggct taggattagt agcacagagt atcccagagg acctttagac ttgccttcgt
     1741 aacttcaagc tgactgtgct ggaagagata gaggtg
//
LOCUS       DAOTON010000644         1776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_51934_length_1776_cov_2.777455, whole genome shotgun sequence.
ACCESSION   DAOTON010000644 DAOTON010000000
VERSION     DAOTON010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51934_length_1776_cov_2.777455"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="P5292_11320"
     CDS             <1..1009
                     /locus_tag="P5292_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39760.1"
                     /translation="VDDFDATASPNGNAIYLFTDDLGNTNVHRYSSTDGGYTWTGSTA
                     LVAGNAANPRVCMDGTRLILNYYGPVLSPATTSIIRSAFYNETVPGTIAPGAGAFADR
                     LPSGPTRPQFESAIVANTVWLFWTETNGIDISLKYMVSSDNGVNYSTEATVSNDAACF
                     GLTTIRSIVDPAVGLAYYHDSVPVAPNYRYSQVRALNPTAFSSPESFSDFDPSCFGGH
                     ETYPAVAAIGNDVGVVWTETDAFASLYFDLRSAITKVETVSPKGDIRVYPNPAGDRLS
                     IRSGQQTMGTRYRIVDLSGQIVQTIQLESGITTIDIHLLASGVYQLVSESGAATRFMK
                     N"
     gene            complement(1107..1739)
                     /locus_tag="P5292_11325"
     CDS             complement(1107..1739)
                     /locus_tag="P5292_11325"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39761.1"
                     /translation="MKPLCRILGFVLVLQLSALPVSRAQVTCDQFCIDDISLDTVPGI
                     MNLTITLSGDSSTFINYPYPEAVVDQNGDTIGNGSMFFFGQFGNSTSVYPCSTSLTLI
                     PPGFEATILFRYDTLTCLLPYPCIIQSIGPRFTNPVLSVFPIPATDQLMVALKEAKSS
                     MRLQLMDTRGVIVRDWNLVERESDLDIHGIAAGIYFLRPVEGDAVRVVIE"
BASE COUNT          439 a          487 c          447 g          403 t
ORIGIN      
        1 tgtcgatgac ttcgacgcca cggcctcccc gaacggcaac gcgatctacc tctttaccga
       61 cgatctgggc aacactaacg tgcaccgtta cagctccacc gacggcggtt acacctggac
      121 cggcagcacc gcgctcgtgg cgggcaatgc agccaatccg cgcgtgtgca tggacggaac
      181 acggcttatc ctgaattact atggacccgt actttctccc gcaacgacct cgatcattcg
      241 ctctgccttc tacaacgaga ccgtccccgg aaccatcgca ccgggcgctg gcgcgtttgc
      301 ggatcgcctg ccttcgggac cgacacgacc gcaattcgaa agcgcgatcg tggccaatac
      361 ggtctggttg ttctggacag aaacaaatgg catcgacatt tcactcaagt atatggtcag
      421 ttccgataac ggagtgaatt atagtacaga agccacggtt tccaacgacg cagcctgctt
      481 cggtcttacg accattcgca gtatcgtcga cccggcggtc ggattagcct attatcacga
      541 ctccgtcccg gtggctccca actaccgcta cagccaggtc cgggcgctaa atcctaccgc
      601 tttttcttca ccggaatctt tcagcgattt tgatccgtct tgcttcggtg gacacgaaac
      661 atatcccgcc gtagccgcca ttggaaatga tgttggagta gtctggaccg aaaccgacgc
      721 ctttgcatcg ctttattttg atctgcgttc ggctattaca aaagttgaaa ccgtttcccc
      781 gaaaggcgac atccgtgttt atccgaatcc ggccggcgac cgattatcga ttcggtcagg
      841 tcagcaaaca atgggtacgc ggtaccggat cgtcgaccta tccggtcaaa tcgtacaaac
      901 catccaattg gaaagtggaa taacgacgat cgatatccac ctcctcgctt ctggtgtcta
      961 tcaactggtg agcgaatccg gtgctgcaac ccgtttcatg aagaattgat cttccggagc
     1021 gttcgtttta cttaagcacg cataagggaa cctctaataa aaagcgccgg ggttgatgat
     1081 caaccccggc gcttttcttt ttgatttcat tcgatcacaa cccgaaccgc atccccttcc
     1141 acaggtcgca agaaatagat gccggctgcg attccgtgaa tatccaaatc ggactcgcgc
     1201 tcaaccagat tccaatcccg aacaatcacg cctcgtgtat ccatcagttg taaacgcatc
     1261 gatgacttcg cttctttcaa cgcgaccatc aattggtcgg tcgccgggat cggaaatacc
     1321 gataggacag ggttcgtgaa ccggggtccg atggactgga tgatgcaggg ataaggcaat
     1381 agacaggtca aggtatcgta acgaaacagg atggtggctt caaaaccagg cggtattaaa
     1441 gttaaagacg tagaacatgg atacacggag gtggaatttc caaactggcc aaagaagaac
     1501 atggatccgt taccgatggt atctccgttc tgatccacca ccgcttccgg atagggataa
     1561 ttgatgaacg tggagctgtc gcccgataac gtgatcgtga gattcatgat gccgggaaca
     1621 gtatccaggg aaatgtcatc gatacagaac tggtcgcagg taacctgtgc ccgactgaca
     1681 ggcagggcgg acagttgcag gaccagaacg aatccaagaa ttcggcagag tggtttcatg
     1741 gaattgcttc tttaaagcaa atgtatggtc gggcac
//
LOCUS       DAOTON010000645         1775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_51968_length_1775_cov_3.539535, whole genome shotgun sequence.
ACCESSION   DAOTON010000645 DAOTON010000000
VERSION     DAOTON010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51968_length_1775_cov_3.539535"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1775
                     /locus_tag="P5292_11330"
     CDS             <1..>1775
                     /locus_tag="P5292_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39762.1"
                     /translation="ETEQQLRVSRENPRKINLTADIRDLRLRGQFLVQGFNVEQYLMP
                     SSADLSLLFTTLDGLSQVPMKFETNRAIDDRTVERATVNLNDSISKRVKLELVQVRFN
                     YDASALQQLDEYLDAIREYYAADAVLNRAMSRLSTVNPGDMEALDFHNATLTEAEGLL
                     QGLADKGLDDRLPLHTFDPIQFERRLSQLQQDAAQRRVAINQARATLHLFYYNAGLRL
                     LDQHRPLQAREAFRRSLVALPVFAPAAYQLAVLDFEDGRIHESECRIREILENMDPDP
                     DTRRSSEQLAAGIYQGYLQQAFGQLAASRWEDALDPLMKARNLCQDLNGLRCDNRLDS
                     AFRQARGGVYRSMLDQARRDYQTGDLDGAERGIRAAINYQDRHEKYIPSAVDAKEMLN
                     GVYRMRFDRMLNDARAQITQNKYSAAVAKLDEASRIQQQYGFPLPDQYMELRRSAAKP
                     LVLQALSNGNVQAAANNLSGARVTLREAQALQLQNGLDIDADVNATLDQLRKRIFSKE
                     CENAQRSMDSLASASRQSMELGDYILADEQLERAIRLAADYKDCALNDQAFKAVRDSI
                     RPVVVFGRELRNVNALQSNGRYAEA"
BASE COUNT          407 a          486 c          514 g          368 t
ORIGIN      
        1 ggagacggaa cagcaactgc gggtatcgcg ggagaatcca aggaagatca acctcactgc
       61 cgacatccgg gatttgcgcc ttcgcggtca gttcctggtg caggggttca atgttgagca
      121 atacctgatg ccgtcctcgg ctgatctttc cctcttgttt acgacgctgg acggattgag
      181 tcaggtgccc atgaaatttg aaaccaaccg cgcgatcgac gatcgaactg ttgagcgagc
      241 cactgtgaac ctgaacgaca gcatcagcaa acgggtcaaa ctggagctgg tacaagttcg
      301 tttcaactac gatgcgagcg cgctgcagca gctcgacgaa tacctggatg ccattcggga
      361 gtattacgct gcggatgccg tactgaacag ggccatgagc cgtctgtcaa cggtgaaccc
      421 cggtgacatg gaagccctcg atttccacaa cgccacgctg accgaagcgg aaggcttgtt
      481 gcaaggactt gccgacaaag ggctcgacga ccgtttgcca ctccacacgt ttgatcccat
      541 acaattcgag cgccgtctga gtcagttgca gcaggatgcc gcccagcgac gagtcgccat
      601 caatcaggcg cgtgcaacgc tccatttgtt ctattacaat gccggtctgc gcctattgga
      661 tcagcatcgt cctttacagg cacgggaagc tttccgtcgg tctctggtcg cgcttccggt
      721 ttttgctccg gctgcctatc aactggccgt actggatttc gaagatggca ggatacatga
      781 atcagaatgt cgcatccgcg agatcctgga aaacatggat cccgacccgg acacccggag
      841 atcctccgag caactggctg ccggtattta ccagggttat ctgcaacagg cttttggaca
      901 attggctgct tcccgatggg aagatgcgct ggatccgttg atgaaagcac gcaacctctg
      961 tcaggacctg aacggtctgc gctgcgacaa ccggctggat tcagcttttc gccaggctcg
     1021 aggtggtgtg tatcgctcga tgctggatca ggcgcgtcgc gattaccaga ccggtgacct
     1081 cgatggcgcg gaacgtggga ttcgtgctgc cattaactat caggaccggc atgaaaagta
     1141 cattcccagt gcagtcgacg cgaaggagat gctgaacggg gtgtaccgga tgcgtttcga
     1201 tcgtatgctg aatgacgcgc gcgcgcagat tacccagaac aagtatagcg ctgcggtcgc
     1261 caaactggac gaagcaagtc gcatccaaca gcaatacggt ttccctttgc cggatcagta
     1321 catggaattg cggagaagcg cggcaaagcc cttggtgttg caggcacttt cgaatggtaa
     1381 cgtacaagcc gccgccaata acctctccgg tgcacgcgta accctgcggg aggcgcaggc
     1441 cctgcaattg cagaacggtt tggacatcga cgcggatgtc aacgccaccc tggatcagtt
     1501 acgtaaacgg attttctcca aggaatgtga aaatgcacag cgcagcatgg attccctggc
     1561 aagtgccagc cgacaatcga tggagcttgg agattacatc ctcgccgacg agcagctgga
     1621 gcgggccatc cgactggcgg ccgactacaa agattgcgcg ttgaacgatc aggccttcaa
     1681 ggcggtccgg gatagtattc gaccggtggt cgtcttcggt cgcgaattgc gcaacgtaaa
     1741 cgccttgcaa agcaatggac ggtatgcgga ggcaa
//
LOCUS       DAOTON010000646         1772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_52089_length_1772_cov_3.214910, whole genome shotgun sequence.
ACCESSION   DAOTON010000646 DAOTON010000000
VERSION     DAOTON010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52089_length_1772_cov_3.214910"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(638..1534)
                     /locus_tag="P5292_11335"
     CDS             complement(638..1534)
                     /locus_tag="P5292_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRS39763.1"
                     /translation="MKALPSGKQVSQILLEPMQLKALAAYLALIDLKRYSPATRRSYG
                     TAFRYFLSGFPGRKPSVISKAEIMDWLLRQQKVHDWSGSYQNLMINAIKFFYEQLLKR
                     PREEYDLPRAKEPHLLPNILSVEEVLRLFECTGNRKHRLLLMLAYSGGLRISEVVNLE
                     LADVDSRRMVIHLRAAKGKRDRVVMLSEVFLEELREYYRRYRPKRWVFEGQTGERYSV
                     RSIQLVFRAAVKKAGIRKRITFHGLRHCFATHLLEGGTDLRRIQELLGHRSIKTTLRY
                     THVATTDLAKVTSPLDKAKQRK"
BASE COUNT          380 a          448 c          460 g          484 t
ORIGIN      
        1 aggtcggctg tctgatggtt cgtttgtccc gtcactttat ctttatttct gtcgttcatt
       61 cttgctgtgt cgtcttttag gcttgcaggt aacggatagg agctttacga cgggctcaat
      121 gtgtcgatga ttattgtacg ttagcctgtt agcctggcgt aaagctcgtg ttatccgatg
      181 ttggcccggg ttctgaggtg cactgtaagt aggaaacagt tttaataaaa agtgcttgtg
      241 caaaagtgtg ttttgcgcag gcacttttta tgtcagccga tgcgctgacc cgtaacccgg
      301 ccacgaagct ggaagttaaa gaaattctat tatatgcggt tagaactgtg ggttttaacc
      361 aagaactttg ctgcccgcct tccacggatt cattgaaata tatttcattc atccgtggaa
      421 ggcgggcagc actttccggt taattacacc ctcctgcgga gggttgcggg acagcgtatc
      481 ggatgaaaac tgttcgttgc ttacttctaa tacaggccag ggccaatttc ggatcaacgc
      541 tcaaatagac gaaggtttat ttcgtgttat ttctctggat tctttgattt cacccgaata
      601 tacaaacaat cgggcagatt ggcgaaatgc gtgttattca ctttcgctgt ttcgctttat
      661 ccaacgggct ggtcactttg gcgaggtcgg tggtggcgac atgggtgtag cgcagggtgg
      721 ttttaatgga gcgatgccca agcagttcct ggatccggcg caggtcggtg ccgccttcca
      781 gcaagtgcgt cgcgaaacaa tgccttaggc cgtggaaggt gatccgtttc ctgatgccgg
      841 ctttctttac cgccgcccgg aacacgagtt gtatgctgcg tacactgtac cgctccccgg
      901 tctggccctc aaagacccac cgcttcggcc ggtagcgccg gtagtattcc cgcaactctt
      961 cgaggaacac ttcgctgagc atcaccacgc ggtcgcgctt ccccttcgcg gccctcaggt
     1021 ggatgaccat gcggcggctg tctacgtcgg ccagctccag gttgacgact tcactaattc
     1081 gtaatccgcc gctgtaggcc agcatcagca agagccggtg cttgcggtta ccggtacatt
     1141 caaataggcg caacacctct tccacactca ggatgttggg tagcagatgc ggctccttcg
     1201 ccctgggcaa atcgtattct tcgcgcggtc gtttcaacaa ctgctcgtaa aagaacttga
     1261 tggcgttgat catcaggttc tggtaactcc cggaccaatc gtgcaccttc tgctggcgta
     1321 aaagccagtc catgatctcc gccttgctga tcacgctggg ctttcgaccg ggaaatccgc
     1381 tcaggaaata gcgaaaggcc gtgccgtaac tccggcgcgt agccggactg tatcgtttga
     1441 gatcgatcag cgcgaggtaa gccgccagtg ctttcaattg catcggctcc aacaggatct
     1501 ggctgacctg cttgccggat ggaagcgctt tcatttcccc aaggacgctg ttccggttcg
     1561 gcctctcaat cgcttaaatg cgggaaaatc gtcggccaag acgggtctac agcgtggtag
     1621 cgggaattcc ttctgcttga tgcgatgatt ttatcttatt agttatcaat attttacaca
     1681 aaaaaggtaa atacaatccg tgaatacttg caattattac aatatgttca tatatttgca
     1741 ttagaattca gccagtaacc atgatcgccg aa
//
LOCUS       DAOTON010000647         1771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_52144_length_1771_cov_2.439394, whole genome shotgun sequence.
ACCESSION   DAOTON010000647 DAOTON010000000
VERSION     DAOTON010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52144_length_1771_cov_2.439394"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..>1771
                     /locus_tag="P5292_11340"
     CDS             60..>1771
                     /locus_tag="P5292_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRS39764.1"
                     /translation="MYTSSAYSGWSCVYTSGNRDGFELDDVMILPLQVDSVAPRCVQL
                     ATESDRSLRLLFDETIEPGSAANTIHYRLDPGAILPLTAQRDALDTKAILLEFANAFQ
                     AGQTYSLTVSGLADAAGNTVRDTTLSFVYTPPVPVLAGDAVFNEIYFEPTASAGLPYA
                     EYVELYNRSDHALSLQDWSLSDGSATAIFPEMTIAAGTYLLVTDEDAIGLLSAFGPAV
                     GLQDFPGLNNDVGDRLVLRDRDGQVVDAVTFSNDTYHDGSRDDGGWSLERIDPDFLCE
                     NEDNWRASYDLSGGTPGEANVVRNVFQDTRRPFLKNAEWIDGLTTRLWFSEPMDTASM
                     TYPDRYTLHRHDETTLSAATVQADVEGRYADLRWPSPVDTGLSWISVSGLLLDCPGNA
                     AYQDQRVPLGIGMPPMSGDVVINEVLFDAPDDASDFVELYNGSEKLLDLKDWTIAEVS
                     AMEPYVVQSVKKLTNENLLLAPGGYLVLTETPLELQRHYPYYDRFCFLDVDDLPDFNS
                     TEGGILLAGAGGDTLDFLVYSDDWHYPLLSITKGVSLERMRVTGPTQSADNWHSAAAT
                     VGYA"
BASE COUNT          352 a          490 c          528 g          401 t
ORIGIN      
        1 atttttctgg atccaaccgg tggaaccgac tatttgttcg ccgcgctggt gcggaatcct
       61 tgtacacatc gtccgcctat tccggctgga gttgcgtcta taccagcggt aaccgcgacg
      121 ggtttgaact ggatgatgtg atgatccttc cgttgcaggt ggattccgtc gcgccgcgct
      181 gcgtgcagct cgcaacggag tcggaccgca gcctgcgttt attgttcgat gaaacgatcg
      241 agccaggctc cgcagcgaac acgattcact accggctcga cccgggagcg atcctgccgc
      301 tcacggcaca gcgcgatgcg cttgacacga aagccatcct gctggaattc gcgaatgctt
      361 ttcaggccgg ccagacgtat tcgcttaccg tatcgggctt ggcggatgcg gcgggtaata
      421 cggttcgcga cacgacgcta agctttgttt atacgcctcc ggttccggtg ctggcaggcg
      481 acgccgtttt caacgagatc tacttcgagc cgacagcctc cgccggcttg ccctatgccg
      541 agtatgtgga gttgtacaac cggtccgatc atgcattgtc gctgcaggac tggtcgctct
      601 cggacggaag tgcaacggcc atctttccgg aaatgaccat agccgcagga acctatctgc
      661 tggtaaccga cgaggatgct atcggcttgc tgtccgcatt cggaccggcc gtcggactgc
      721 aagattttcc ggggctgaac aacgatgtcg gggatcgtct ggtcctgcgc gaccgtgacg
      781 gacaggtagt cgacgcggtc accttcagca acgataccta tcacgatggt tcccgcgacg
      841 atggcggttg gagcctggaa cggatcgatc cggattttct ctgcgagaac gaagacaatt
      901 ggagagccag ttatgacctt tcaggaggta cgccgggtga ggcgaatgtt gtgcggaatg
      961 tgttccagga tacccggcgg cccttcctga aaaatgccga atggatcgac ggtctgacga
     1021 ctcgcctctg gttctccgag ccgatggata cggcaagcat gacatatcct gatcgataca
     1081 ccctgcaccg gcacgacgaa acgaccctta gcgcggcaac ggtacaagct gatgttgagg
     1141 gtcgatatgc cgacttgcgt tggccatctc cggtggatac cggcttgtcc tggatcagcg
     1201 tatcgggttt gctgttggat tgcccgggca atgcagcgta ccaggaccag cgggtaccct
     1261 tgggcattgg aatgccgcct atgtccggcg atgtagtcat caatgaagtg ctgttcgatg
     1321 cgccggatga tgcatccgat ttcgtggagt tgtataacgg ttccgaaaag ttactggacc
     1381 ttaaagactg gaccatcgcg gaagtatcgg cgatggaacc gtatgtggtt caatcggtga
     1441 agaagctcac gaatgaaaat ctgctgctgg cgcccggcgg ttacctggtc ctgaccgaaa
     1501 cgccgctgga attgcaacgg cattatccct actacgaccg cttctgtttc ctggatgtcg
     1561 acgatctccc ggatttcaat tccaccgaag gcggaatcct cttggccggt gcaggagggg
     1621 ataccctgga ctttctggtc tattcggatg actggcatta tccgttgctc agcatcacca
     1681 agggcgtgtc gcttgagcgc atgcgtgtta ccggacctac acaatcggcg gacaactggc
     1741 attccgccgc cgcgacggtc ggctatgcga g
//
LOCUS       DAOTON010000648         1771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_52150_length_1771_cov_2.036131, whole genome shotgun sequence.
ACCESSION   DAOTON010000648 DAOTON010000000
VERSION     DAOTON010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52150_length_1771_cov_2.036131"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="P5292_11345"
     CDS             <1..876
                     /locus_tag="P5292_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRS39765.1"
                     /translation="GLTIREIMAHQGGLVSWIPYWKKTVSANGPDSLYYRRTPDERFS
                     VRVSDSLFTFPQLADSILDWSVKSPAGERGKYVYSDLGPIFLRFALERVADKPIDQFL
                     EEQFFSPLQLNNTLYKPWQNFSADRIIPTERDTAFRKKLVLGDVHDPAAAMQGGVSGN
                     AGLFSNAGDLAVIFQMLLNHGSYAGRQYLKPNTVSMFTRRQFTQGNNRRGLLFDKPEI
                     DPSKSSPCAASASPETFGHQGFTGTCVWADPKEELLVIFLSNRVFPDATNDKLVKMNI
                     RTRIQQAAYDAILDQ"
     gene            914..>1771
                     /gene="bshA"
                     /locus_tag="P5292_11350"
     CDS             914..>1771
                     /gene="bshA"
                     /locus_tag="P5292_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HRS39766.1"
                     /translation="MKIGILCYPTFGGSGVVATELGKALAQKGHQVHFITYSQPVRLE
                     HFAENVFYHEVTVADYPLFEFSPYETALTSKLVDVALFEKLDIVHVHYAIPHASVAYM
                     ARQILLSRGTYLPFITTLHGTDITLVGKNESYNPVVTFAINQSDAVTSVSDSLRRDTF
                     QNFSIEKEIEVIPNFIDLRRFNKMRKDHFRRAIAPNNERLIIHASNFRPVKRVSDVVS
                     VFNRVQEKIPAKLLLIGDGPDRSKVEKECRECSACEHIRFLGKLEAVEEILSIGDLFI
                     LPSETESFGL"
BASE COUNT          418 a          476 c          466 g          411 t
ORIGIN      
        1 ggcctgacga tccgggagat catggctcac cagggaggat tggtttcctg gataccgtat
       61 tggaagaaga cggtttcggc caatggtcct gacagtttgt actatcgccg tacacccgac
      121 gagcgcttct ctgtcagggt atcggacagt ctctttacat ttcctcaatt ggcggatagt
      181 atcctggatt ggtcagtcaa aagcccggcg ggagagcggg gcaaatacgt gtattccgat
      241 ctcggcccca ttttcctgcg ctttgcgttg gagcgtgtcg cggacaaacc catcgatcaa
      301 ttcctggaag agcaattttt cagtccactg caactgaata atacattgta taaaccctgg
      361 cagaacttct ccgcagaccg catcattccg acagagcggg atactgcctt tcggaaaaaa
      421 ctagtgctgg gtgatgtgca tgatcctgcc gcagccatgc agggcggcgt gtcgggcaac
      481 gcgggtcttt tcagcaatgc gggtgatctc gccgtcatct tccagatgtt gctcaatcat
      541 ggaagttacg cgggacgtca gtacctgaag ccgaatacgg tgagtatgtt cacccgcagg
      601 caattcaccc aaggcaacaa ccgccgtggc ttgttgttcg ataaacctga aatcgatcct
      661 tccaagtcca gtccgtgtgc agcatccgca tctcctgaaa cattcggaca ccagggattc
      721 accggaacct gtgtctgggc tgacccgaag gaggagttac tggtgatttt cctttccaac
      781 cgggttttcc cggatgcaac caacgataag ctggtgaaaa tgaacattcg gacacggatc
      841 cagcaggcgg cttacgacgc gatactcgac cagtaaggac ggaagtcgta cctttggagg
      901 gaatccctcc cacatgaaga tcggcatctt atgttaccct acgttcggcg gcagtggagt
      961 cgttgcgacg gaattgggta aagccctggc ccagaaaggg catcaggtgc atttcatcac
     1021 ctacagccaa ccggtgcgat tggaacattt tgccgagaac gtcttctacc acgaggtgac
     1081 cgtagcggac tatccgctct tcgaattcag tccatacgaa accgcgctga ccagtaaact
     1141 ggtcgatgtc gcgctcttcg agaaactgga tatcgtacat gttcactacg ccataccgca
     1201 cgcgtccgtc gcctatatgg cccgacagat cctgctcagc aggggtacct accttccgtt
     1261 catcaccact ttgcacggaa ccgacattac cctcgtcggg aagaatgaaa gttataaccc
     1321 ggttgtcacc tttgcgatca accagagtga cgcggttacc agtgtttccg atagcctgcg
     1381 gcgcgatacg tttcagaatt tcagtattga aaaggagatc gaagtgatcc ccaacttcat
     1441 cgacctccgg aggttcaaca agatgaggaa ggatcatttc aggcgtgcga tcgcaccgaa
     1501 caatgaacgc ctcatcatcc atgcttccaa cttccggcct gttaagcggg tcagcgatgt
     1561 tgtctcggta ttcaaccggg tgcaggaaaa gatcccggcc aaattgttat tgatcggcga
     1621 cggtcccgac agaagcaagg tcgagaagga gtgccgcgaa tgttccgctt gcgaacacat
     1681 tcgctttctc gggaaattgg aagcggtcga ggaaatcctc tcgatcggcg acctgttcat
     1741 tcttccgtcc gagaccgaga gtttcggact t
//
LOCUS       DAOTON010000649         1770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_52180_length_1770_cov_2.681633, whole genome shotgun sequence.
ACCESSION   DAOTON010000649 DAOTON010000000
VERSION     DAOTON010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52180_length_1770_cov_2.681633"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="P5292_11355"
     CDS             <1..682
                     /locus_tag="P5292_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39767.1"
                     /translation="NVRLDIVKSPTDSFQLVRIYSARGGSRKEAVDNASHIDYGFTQT
                     DSSIRFQGFYKLAETEPFRFQKVQLVLRIPVGAKIYLDRGLEDFIYDIDNVQNIFDRD
                     MLGRTWLMTADGLTCVDCTGDEDVVGGGKFKDDDFRGRIRINSDEGAEVKIDDNGVRI
                     IGPEGEKVIIDSTGVRINGKKLRGPVPPLPPKPGDSIRISPNGVRISLEDNKQVTPAI
                     QSRGISVV"
     gene            805..>1770
                     /locus_tag="P5292_11360"
     CDS             805..>1770
                     /locus_tag="P5292_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS39768.1"
                     /translation="MNQSIRLEPIAIADAEWMYRLRSNPVVNRYIEYTRPDSLEKMQE
                     TLQRIIEVNALERWNFNKILRAKDDQPLGTICLWNYNEDRTRAEFGFELFPEEHGKGI
                     MSEALSLMLDFARKVEQLKEVGAWTHRDNSAARRLLERHGFERDLDAESKAAADDNLK
                     SYVIYSRRLVPFVQPDRLETTRLRQMPFTEGDAQFILQLLNTDGWKRYIGDRGVTDEA
                     SAIAYLQRSPMRLEREQGMSFYKVCKKDDGTPIGMCGLILRDYLPGPDLGFAFLPEAE
                     GKGYAYEAAMAWIPLVKSIFGYETVYAITVPENERSIRLLKKLLFE"
BASE COUNT          429 a          462 c          504 g          375 t
ORIGIN      
        1 caatgttcgg ttggatatcg taaaaagccc gacagactcc ttccagcttg tccggatcta
       61 ttcggcacgc ggaggttcca ggaaagaagc ggtcgacaat gcttcgcaca tcgactatgg
      121 cttcacccag acggattctt ccatccgctt ccagggattc tataagctgg cggaaaccga
      181 accgttccgc ttccagaagg tgcaactcgt actccgaatt ccggttggcg cgaagattta
      241 tcttgaccgt ggactggaag acttcatcta cgacatcgac aacgttcaaa acatcttcga
      301 ccgcgacatg ctgggccgta cctggctgat gaccgccgat ggtttgacct gcgtggattg
      361 taccggtgac gaagacgtgg tcggcggcgg caagttcaag gacgacgatt tccgcggccg
      421 catccgcatc aacagcgacg aaggcgccga ggtgaagatc gacgacaacg gtgtccgcat
      481 catcggtccc gaaggggaaa aggtgatcat cgacagcacc ggcgtacgca tcaacggcaa
      541 gaaacttcgc ggtcccgtac cgccccttcc tccgaagccc ggcgacagta tccgaatctc
      601 ccccaatggc gttcggatca gtctcgagga taacaagcaa gtaactcccg cgatccaatc
      661 gcggggaatc agtgtggtct gagtatacga tggtcttgga cctgtaaaaa gggttccggg
      721 gagtggtccg gagccctttt ttcttttcta agctgagcgc tgacgaacgc agcctagcac
      781 gattcttcgg atattgccgt ccctatgaac cagagcatca gactggaacc gatcgcgatc
      841 gcggatgcgg aatggatgta ccggttgcgc agcaaccctg tggtcaatcg ctacatcgaa
      901 tatacccggc cggatagcct ggagaaaatg caggagacgc ttcagcgcat cattgaagtg
      961 aacgcgctgg aacgctggaa cttcaataag atcctgcgcg cgaaggacga tcagccgttg
     1021 ggtaccattt gcctttggaa ttataacgaa gacagaacca gggcggagtt cgggttcgaa
     1081 ctttttccgg aggaacatgg gaagggaatc atgtccgaag cgctctcgtt gatgttggac
     1141 tttgcacgca aggttgaaca gttgaaagaa gtcggggcgt ggacccaccg ggacaattcc
     1201 gctgcccggc gactgttgga acggcatggc ttcgaacgtg accttgacgc ggaatccaaa
     1261 gctgccgcag acgataatct aaagtcgtac gtcatctaca gccgacgatt ggttcctttc
     1321 gttcaacccg atcggttgga aacgacgagg ctccgacaaa tgcccttcac ggaaggtgac
     1381 gcgcaattca tccttcaatt attgaatacg gatggttgga aacgctacat cggggatcgc
     1441 ggtgtaaccg atgaggccag cgcgattgcc tatttgcaaa ggagtccgat gcggttggag
     1501 cgggagcagg gcatgagttt ttataaggta tgcaagaaag acgacggcac cccgatcggt
     1561 atgtgtggct tgatcctgcg cgactacctg ccgggcccgg acctcggatt cgcatttttg
     1621 ccggaagcag aagggaaagg ctacgcctac gaagccgcca tggcctggat cccactggtg
     1681 aaatcgatat tcggttatga aacggtctat gcgatcacgg taccggaaaa tgagcggtcc
     1741 attcgtctcc ttaaaaaact attgttcgaa
//
LOCUS       DAOTON010000650         1769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_52205_length_1769_cov_3.838973, whole genome shotgun sequence.
ACCESSION   DAOTON010000650 DAOTON010000000
VERSION     DAOTON010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52205_length_1769_cov_3.838973"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="P5292_11365"
     CDS             <1..458
                     /locus_tag="P5292_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39769.1"
                     /translation="QLWRTSPTSEVIVQRNADGRLELLDFSGTLLPAPDSSYVTDFCL
                     LTDSLNSQLLVIRNDSLFSYAGDGTRSFRGVSPLSGLRCSSGPASALFALFSNDAKKT
                     WVYRGSGPASAGYPVNGIPGKEVDGFQLFHEDKMVLTDGETLLYLYTLE"
     gene            478..>1769
                     /locus_tag="P5292_11370"
     CDS             478..>1769
                     /locus_tag="P5292_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39770.1"
                     /translation="MKKIVAAFILFSFTISACIKKDDFNFEDPSYSIWQPEVALPLVN
                     SVLTMDDLLGRADSGTLEIDSATDRVILIYDGNVYSVNGEDFLPLLDQQDLGEFRLNA
                     PDSAQLTGSGTLLQQLPRDFPFTVANGEELDSVVLKNGMLNVSLINTAPHGGTFSVRI
                     PAARKNGVVFDQSVLFTPANGTSTSASGSFDLSGYSIDFTAGTSSNRLDIVYEADWTN
                     SGLNLACTDRPLSFTIDYDQLRLSAAYGDFRQRSLSISVDSSELTIFQNTLGGTIHFD
                     EPKFVFNIRNSFGMPVRAILQNLVSIPRTGPNIPVTGAIPNPIPVGVPGAPGLVAATS
                     FTLDKNNSNVQTVVNAEPEYISYDVDATTNVPSGTRNFLLDTSRFSVDLRVELPLVGY
                     ASGLSVQDTADFELENVEELQSAIFRLYTSNGFPAQSWT"
BASE COUNT          395 a          510 c          440 g          424 t
ORIGIN      
        1 ttcagctttg gcgtacatca ccgaccagcg aagtgatcgt gcaacgaaat gcggatggcc
       61 gacttgaact ccttgacttt tccggaacgc tactgcctgc acctgattca tcctacgtga
      121 ccgatttctg cctgttgacc gacagcctga acagtcaact gctggtcatc cggaacgaca
      181 gcctgttcag ttacgccggt gatggaacga gatcgttccg tggcgtctca ccgttatccg
      241 gccttcgttg cagctcaggg cctgcttcag cgcttttcgc gctcttttcc aacgatgcaa
      301 agaaaacctg ggtctaccgc gggtcaggtc cggcttcggc gggctacccg gtgaatggaa
      361 ttccgggcaa agaggtggac ggatttcagc tcttccatga ggataaaatg gtcctgacgg
      421 atggagaaac actgctctat ctctatacat tggaatagcg actccgacac cactaccatg
      481 aaaaaaattg ttgccgcgtt tatcctcttt tcattcacga tctcggcgtg tatcaagaag
      541 gacgatttca atttcgagga tccctcctat tcgatttggc agcctgaagt cgcgctgccc
      601 ctggtaaatt ccgtgttgac catggacgat cttctgggaa gagccgactc cggcactttg
      661 gagatcgact cggctacgga tcgcgtcatc ctgatctacg atggcaacgt atattcagtc
      721 aatggagaag attttctgcc cttattggac cagcaagatc ttggtgaatt ccggctgaat
      781 gccccggaca gcgcgcagtt gaccggttca ggaacactgt tacaacaact accgcgtgat
      841 ttccccttta cggttgccaa tggtgaggaa ctggacagcg ttgtccttaa aaacggaatg
      901 ctgaacgtat cactgatcaa taccgcaccg catggcggta ctttctccgt tcggataccg
      961 gccgccagga agaatggcgt ggtgttcgac cagtccgtac tcttcacacc cgccaacgga
     1021 acgtccacct ccgcaagtgg cagtttcgat cttagcggtt attcgatcga ctttacggcc
     1081 ggcacttcca gcaaccgact ggacattgtg tatgaagccg actggaccaa cagcggactc
     1141 aacctggctt gtaccgatcg tccgctctcc tttacgatcg attatgacca gttgaggtta
     1201 tccgccgcct atggcgattt ccgtcaacgc agcctgagca tttcggtcga ctcgagcgag
     1261 ttgaccattt tccagaacac cctgggtggt accattcact tcgacgaacc gaaattcgta
     1321 ttcaacatcc gcaacagttt cggcatgccg gtcagggcca ttctacaaaa ccttgtctcc
     1381 attccgagaa cggggccgaa tatccctgta accggcgcaa tccccaatcc gataccggta
     1441 ggtgtccccg gcgctccggg ccttgtcgca gcgacttcgt tcaccttgga taagaacaac
     1501 agcaacgttc aaaccgtggt caatgcggaa cctgaataca tcagttacga tgtcgacgcg
     1561 accaccaacg ttccttccgg cacccggaat ttcctactcg acaccagcag gttcagcgtc
     1621 gacttgcgcg tcgagcttcc gctggttgga tacgccagcg gcttgagcgt gcaggatacc
     1681 gccgactttg aattggaaaa tgtcgaggag ctccaatcgg ccattttccg actctataca
     1741 tccaacggat ttccggccca gtcgtggac
//
LOCUS       DAOTON010000651         1769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_52217_length_1769_cov_2.757876, whole genome shotgun sequence.
ACCESSION   DAOTON010000651 DAOTON010000000
VERSION     DAOTON010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52217_length_1769_cov_2.757876"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="P5292_11375"
     CDS             complement(<1..455)
                     /locus_tag="P5292_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRS39771.1"
                     /translation="MNELLAIMKQELLLTGTLLLVLVLKLAGMPSSNNKWNGWMQFLL
                     LANLIGGWIGNVSGNLFGDMYVTDPSLVLQKNLLLLATLVIVSLSTTWLERHRHLPEF
                     YLLLLSTLLGMFWMISSRNLLMFYLSLELATIPLAAMVNFDLGKRISSEA"
     gene            complement(592..>1769)
                     /locus_tag="P5292_11380"
     CDS             complement(592..>1769)
                     /locus_tag="P5292_11380"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRS39772.1"
                     /translation="KERPKEFFFLLMLLSAGAYGFFIATDLFLLFFFLEMAVVPKYLL
                     IGIWGSGRKEYSANKLVLLLMGGSALVFVGLLGVAYASGNLAPSFDWMNAAALRMPLD
                     KQLFFFPFLFVGFGVFTALFPFHTWVPDGHSSAPTAASMFLAGISMKLGGYGCLRVAA
                     YLMPEAARIYSPYIVILATIAILYGAFATLMQKDLKYINAYSSVSHCGFVLLGIGMLT
                     PTAIRGAVLQMVSHGLMTALFFAAIGMLYERTHTRKVEELGGLMKAMPFVGTLFFLAG
                     LCSLGLPGLSGFVAEMTVFAGSWERPDTFYRIATVLSCMSIVVTAVYILRAIGSSIMG
                     PLKKADYAELSDAGSSERFAAVLLMAGILIVGLAPFWLNDLIGPATTIITEQLGRWM"
BASE COUNT          496 a          518 c          431 g          324 t
ORIGIN      
        1 gcctcggacg aaattcgttt acccagatcg aaattcacca tagcagccag cggaatcgtt
       61 gccaattcga gcgacagata aaacatcaac aggttccggc tggagatcat ccagaacatg
      121 cccaacaggg tcgacaacaa caagagataa aattccggta agtgacggtg gcgctccaac
      181 caggtggttg aaagtgaaac gatcaccaac gtagcgagca agaggagatt tttctgcaaa
      241 accagcgagg ggtcggtgac atacatgtcg ccgaacaggt tgccggaaac attcccgatc
      301 cagcctccga taaggtttgc caagagtaaa aactgcatcc agccgttcca cttgttgttg
      361 gacgacggca ttccggccag tttcagcacc agtaccaaca gcagcgtgcc cgttaggagc
      421 agctcctgct tcatgatggc caatagctcg ttcatttacg ttcgttcaat tccgggatca
      481 gctcccattt tatcagatga actaacaact cctcagttta acgttcaagg ttccttgaac
      541 ttctccctga tacatacgcg caatatcaac tcccgcgcct cgcaactcct gtcacatcca
      601 gcgccccaac tgctcggtga tgatcgttgt ggcgggtcca atgaggtcgt tcaaccagaa
      661 cggcgccaaa ccgacgatca gtattcctgc catcagcaaa actgcggcaa agcgctcgga
      721 agatccggca tccgaaagct cagcataatc cgccttcttc aacggaccca tgatcgatga
      781 accaatagca cgcaggatgt aaacggcggt aacaacaatc gacatacaac tcaggaccgt
      841 cgcgatgcga tagaaggtat cgggtcgttc ccaggatccc gcaaacacgg tcatctccgc
      901 cacgaatccg ctcagtcccg gcaatccaag agaacacaag ccggcaagaa agaacaaggt
      961 gcccacgaac ggcatcgcct tcatcaggcc gcccagttct tcgacctttc gtgtatgggt
     1021 gcgttcatac aacatcccga tcgcggcgaa gaagagtgcc gtcatcaggc cgtgcgacac
     1081 catttgcaac actgctcccc ggatggctgt cggagtcaac atgccgatac ccaacaacac
     1141 gaatccacaa tggctgaccg acgaatacgc gttgatgtac ttcaggtcct tctgcatgag
     1201 tgtagcgaac gctccataca ggatggcgat cgtggcgagt atgacgatgt aaggagagta
     1261 aatccgcgca gcttccggca tcaggtaagc cgccacccgc aaacatccat agccacccag
     1321 cttcatggag atcccggcga gaaacatgga tgcggccgta ggcgccgatg agtgtccgtc
     1381 cggcacccag gtatggaacg gaaacaaggc cgtaaaaacg ccgaagccca cgaagaggaa
     1441 cggaaagaaa aacaactgct tgtccagcgg catccgcaat gcagcggcat tcatccagtc
     1501 aaaggacgga gcaagattgc ccgacgcata ggctactccc agcaggccga caaacaccag
     1561 cgcagaaccg cccatgagca gcaacaccaa tttattggcg ctgtattctt tccgtccgct
     1621 cccccagatg ccgatcaaca gatacttagg aaccacggcc atctccagga agaagaacaa
     1681 caggaacaga tcggttgcga tgaaaaatcc ataagccccg gcgctcagca gcatcaggag
     1741 aaagaaaaat tccttcggcc gttccttga
//
LOCUS       DAOTON010000652         1766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_52322_length_1766_cov_3.905319, whole genome shotgun sequence.
ACCESSION   DAOTON010000652 DAOTON010000000
VERSION     DAOTON010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52322_length_1766_cov_3.905319"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="P5292_11385"
     CDS             <1..727
                     /locus_tag="P5292_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39773.1"
                     /translation="VSWPNLLYYTLAPFRWSELTPVGGLFALVGCWHRFRHGFSRADA
                     MMGIWYAGVWLFLCGIPIQNPRFALPMYVPIAWMMIAGLMALRPWLTRKAGIAVIPIY
                     IGIAGWLGMHKWAGFVGEKDRMLSEARYFDQRIPPQARILSWDYFEAFHIYLPARKVS
                     TIHGLNRQQLQELLDREGELYLLFDEARLTRAWAGLEPALAYQDLLLRNPSELLERQG
                     RYSLRRCTATGRSGAPNSVPSPD"
     gene            738..>1766
                     /locus_tag="P5292_11390"
     CDS             738..>1766
                     /locus_tag="P5292_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRS39774.1"
                     /translation="MKKKFELLEKKIAEAKQGGGEARIAAQHKKGKLTARERLHFLLD
                     EGSFEEIGMLVTHRATDFGIDKEKYLGDGVVTGFGTINGRLVYVFAQDFTVFGGSLSE
                     THAKKICRIMDLAMQNGAPVIGLNDSGGARIQEGVVSLGGYADIFYRNTLASGVIPQI
                     SAIMGPCAGGAVYSPAITDFILMVENTSYMFVTGPNVVKTVTHENVTSEELGGAMTHA
                     TKSGVTHFTAANGLEAIQMLKRLLSYIPQNCEEEPPSIPYEAGDESRPGLNDLIPENP
                     NQPYDMRDVITQVIDTDSFFEVHKDFAENIVVGFARLAGKSIGIVANQPAQLAGVLDI
                     HASTKAARF"
BASE COUNT          389 a          453 c          537 g          387 t
ORIGIN      
        1 ggtgagctgg ccgaacctgt tgtattatac cctcgcgccg tttcgctggt cggaactgac
       61 gcctgtcggc gggttgtttg cgctggttgg ctgctggcat cggtttcgac acggattttc
      121 gagagccgat gcgatgatgg ggatctggta cgccggcgtt tggctgtttc tttgcggcat
      181 tccgatccaa aatccccgtt tcgcactgcc tatgtatgtt cccatagcct ggatgatgat
      241 agcgggtttg atggccttgc gaccctggct gacgcgaaaa gccggaattg cagttatacc
      301 tatatatata ggtatagcag gttggctggg aatgcataaa tgggctggat ttgtcgggga
      361 gaaagaccgg atgttatccg aagcccggta ttttgatcag cggattccac cccaggcaag
      421 gatcctgagc tgggactact tcgaagcctt ccatatctat ttacccgcgc gaaaagtcag
      481 taccatccat gggctgaacc ggcaacaatt acaggaattg ttggaccggg aaggggagtt
      541 gtacctgctc ttcgacgaag cacgattaac ccgggcctgg gcaggacttg aaccggccct
      601 tgcctaccag gacctgttgc tgcgtaaccc gtcggaattg cttgaacgac aaggcaggta
      661 cagcttacgc cggtgtacgg cgacgggcag atcgggagcg cccaattccg taccttcgcc
      721 ggactgaatc gccatctatg aaaaagaagt tcgaactcct ggagaaaaag atcgcggagg
      781 ccaagcaggg tgggggagaa gcgcggatcg ctgcgcaaca caaaaaggga aagctgaccg
      841 ctcgcgagcg tttgcatttt ttactcgatg aaggatcgtt cgaggagatc ggcatgctgg
      901 tgacccatcg tgccaccgat ttcggtatcg ataaggagaa gtatctgggc gatggtgttg
      961 tgaccgggtt cggtacgatc aacggacggt tggtctatgt attcgcgcag gacttcaccg
     1021 tattcggcgg gtcgttgtcg gagacgcacg ctaagaagat ctgtcggatc atggacctcg
     1081 ccatgcagaa cggcgcaccg gtgatcggat tgaacgattc cggcggggcg cgtatccagg
     1141 aaggagtcgt ttcgctgggc ggttatgccg acatctttta ccgcaataca ctggcatccg
     1201 gtgtgatccc gcagatctcc gcgatcatgg ggccttgcgc cggaggcgcg gtgtattctc
     1261 cggcgatcac ggatttcatc ctgatggtcg agaacacttc ctatatgttc gtgacgggac
     1321 cgaacgtggt caagaccgtg acacatgaga acgtgacctc cgaagagctg ggcggcgcga
     1381 tgacgcacgc gaccaagtcg ggtgtgacgc acttcacggc agcaaacgga ttggaagcga
     1441 tccagatgct caagcggttg ctgagctaca ttccgcagaa ttgcgaagaa gaaccgccat
     1501 ccattccgta cgaagccggt gacgagtcac gtcccggact gaacgacctg attcccgaga
     1561 acccgaacca gccatacgat atgcgcgatg tcatcaccca ggtgatcgac acggattcct
     1621 tcttcgaagt tcacaaggat ttcgcagaga atattgtcgt cggattcgcg cggttggccg
     1681 gaaagagcat cggcattgtc gcgaaccagc ccgcgcaatt ggcgggcgta ctggacatac
     1741 acgcttccac gaaggcggcg cgtttt
//
LOCUS       DAOTON010000653         1764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_52403_length_1764_cov_3.385020, whole genome shotgun sequence.
ACCESSION   DAOTON010000653 DAOTON010000000
VERSION     DAOTON010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52403_length_1764_cov_3.385020"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /locus_tag="P5292_11395"
     CDS             complement(<1..1681)
                     /locus_tag="P5292_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRS39775.1"
                     /translation="MKRILLRLLLPLLPVLFPNLTFAQWMILSDTTWSGTILVDREVT
                     IKPGKVLTVAAGSVLKMSKGASIHASGGGGVRLMGTNVSPVQVLPVIAGEQWGKIEVR
                     DTGSAVEYHFAELYGGQTKIWNGATADLQDSYFHDYHQGDAPIVFSLDAQFVYMGRCH
                     VSNYYEINLVRSLSTVEDCLFEFTTADAIDFDNSPAGTVIRRTTIRYGRGTNIDAIDF
                     GKVDFQPPGSIGKVEQCLIHDISDKGVSVGEGAQFVEITGTLIYRTGAGVAVKDNSRA
                     LIHQNTFYGCEYAIECVEKNAGLGGGKAEAYDNIIWSPLEGPYYLNSTGSLEMRYSDI
                     EGMTDNSRHIFSRDPSFLDAASDDYRLRFGSSCQTAGSHGQPLGAVFPLGGLREDPFT
                     LRLGLPNSVTSLNGDSLLMTYWAAGDSIRSVDLKFSPDGGLTWQVLAQDIPAQEEKWL
                     WHVPNTYSTRCVFQVLDHDVPTRMAKNELPFTILPVGDTTIKADFSAEAGFYLQPVTL
                     SLSAPAGSLIYYTLDGSEPTDRSFLYSGPLSLAGDSIPPGLQEQNVTSTTEA"
BASE COUNT          457 a          467 c          448 g          392 t
ORIGIN      
        1 gagcctccgt cgtggacgtc acattctgct cttgtaaacc cggcggaata ctgtcgccgg
       61 ccagggacaa gggtcccgaa tacaaaaagg atcggtcggt cggttcgctg ccgtcgaggg
      121 tatagtaaat gagagaaccc gcgggcgcgg agagcgataa cgtaacgggc tgaagataaa
      181 atccggcttc agcggaaaag tcggctttga tcgtggtgtc gcctactggc aggatggtaa
      241 agggcagttc atttttcgcc atcctggttg gaacatcatg atcgagtacc tggaatacac
      301 aacgggtgga ataagtattc ggtacatgcc ataaccactt ttcctcttgt gcaggtatgt
      361 cctgtgccag tacttgccag gtcaaaccac cgtccgggga gaatttcagg tccaccgacc
      421 ggatcgaatc acccgccgcc caataggtca tcaacaggga gtcgccatta agactggtga
      481 cggaattcgg caacccgaga cgaagcgtaa atggatcctc ccgtaagccg cccagcggga
      541 acacggctcc gagcggctga ccgtgacttc cggcagtttg acaggacgaa ccgaaacgta
      601 agcgataatc atccgatgcg gcatccagga aggaaggatc acgggaaaag atgtgccggg
      661 aattgtcggt catgccttca atatcgctgt agcgcatttc aaggctacca gtggaattta
      721 ggtaataggg accctctaac ggcgaccaga tgatgttatc ataggcctcc gccttaccac
      781 cacccagtcc tgcatttttc tccacgcact cgatcgcgta ttcacaaccg taaaaggtat
      841 tctgatggat cagcgcacgg gaattgtctt ttacagcgac accagcgccc gttcgataga
      901 tcaaggtacc cgtgatctcc acgaattgcg cgccttcccc tactgacaca cctttatcac
      961 tgatgtcgtg aatcaggcat tgctccacct tcccgatgga acccggtggt tgaaaatcga
     1021 ccttcccaaa atcaatggcg tcgatgttgg tgcctcgtcc atagcgaatg gtggtacgac
     1081 gaatgaccgt acctgccggg gaattgtcga agtcgatcgc gtcagccgtg gtaaactcaa
     1141 agagacaatc ttctacggtg gaaaggcttc gaacaaggtt gatctcgtag taattcgaaa
     1201 catgacagcg acccatatag acaaactgcg catcgagtga aaagacgatc ggcgcatctc
     1261 cctggtgata atcgtggaaa taactgtctt gcaaatcagc cgtcgctccg ttccagatct
     1321 tggtctggcc tccgtataat tctgcaaaat gatattcgac tgcactgccg gtatcgcgta
     1381 cttcaatctt tccccattgc tcaccggcaa ttaccggaag cacttgcacc ggcgagacgt
     1441 tcgttcccat gagccgcact ccaccaccac cggaagcatg gatggaggcg cctttcgaca
     1501 tcttcagaac agaacccgcg gctaccgtca gtactttacc cggcttaatg gtgacctccc
     1561 ggtcgaccag aatcgtacct gaccaggttg tatcgctcaa gatcatccat tgagcaaagg
     1621 tgaggtttgg aaaaagaacc ggtaaaagcg gcaatagcag ccgcaataaa attcgcttca
     1681 tttcacagtg ttcagagcgg aatcaattcc ttacactgcg aaagttaagt cagattaaaa
     1741 tacgagtaaa gcgttggtta aatt
//
LOCUS       DAOTON010000654         1762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_52512_length_1762_cov_2.334505, whole genome shotgun sequence.
ACCESSION   DAOTON010000654 DAOTON010000000
VERSION     DAOTON010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52512_length_1762_cov_2.334505"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="P5292_11400"
     CDS             <1..327
                     /locus_tag="P5292_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRS39776.1"
                     /translation="DTLAHLSLPASYWIDLNRHDAFTAFREMKGPLYIMQGGRDYQVT
                     TDDYKLWEEKLKGRKPTAMKLYPQLNHFFITGEGKSKPAEYNKSANFSETVLNDIYNW
                     ISKDVY"
     gene            352..957
                     /locus_tag="P5292_11405"
     CDS             352..957
                     /locus_tag="P5292_11405"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRS39777.1"
                     /translation="MIRFADILFSGLGLVVLAPLLVLLSVWVRLGSRGAIIFRQQRIG
                     KDAVPFTLLKFRTMYPDADKKGLITVGSHDPRITPAGKFLRKWKLDELPQLWNVFRGD
                     MSLVGPRPEVERYVALYTAEQREVLRVRPGITDKASIVYADENERLSKAGDPEAHYRE
                     FILPDKIRLNMEFIRKPTFGHYLRILWMTFTHLFFRSSAGQ"
     gene            1142..>1762
                     /locus_tag="P5292_11410"
     CDS             1142..>1762
                     /locus_tag="P5292_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39778.1"
                     /translation="MRIKISFLKVHGSSGTVPLHHQKIISAFMDEVIREMPISSPFYN
                     FSSLKGTSKVQNGQIRFLSSKVSLVLSAPDKEFAEEWVRRIFERRLVSFAKLTLVPKA
                     SEVIPDPDFNQVMKYVCISPMVPQGPFESDPEGNIPDPLDPREQDFSDAFYDVLMENM
                     ERAGFTPQQLHDFAEFEITPDPGYIQKILNTHKKFARIYKNNDSLPI"
BASE COUNT          435 a          443 c          454 g          430 t
ORIGIN      
        1 gatacgctgg cgcatctatc gttgccggcc agttactgga tcgatctgaa caggcacgat
       61 gctttcaccg cctttcgtga gatgaaggga cccttgtaca tcatgcaggg tggtcgggat
      121 tatcaggtaa cgacggatga ctacaagctc tgggaggaga agctgaaggg gagaaagcct
      181 accgcgatga aattgtatcc tcaactcaac cacttcttca tcaccggcga agggaagagc
      241 aagccggctg aatacaataa gagcgcgaac ttttctgaaa cggtgttgaa tgacatctat
      301 aattggatca gtaaggatgt ttattgagga accggtatgt tccatcgttt cctgatccgg
      361 tttgccgaca tcctgttttc cggcttgggt ctggttgttc tggcgccctt gctggtcctg
      421 ctgtcggttt gggtacggct gggttccagg ggcgcaatca tcttccgtca acaacgcatc
      481 gggaaggatg cagtgccttt tacgttactg aagttccgga caatgtatcc cgatgccgac
      541 aagaaaggat tgatcacggt tggctcgcat gacccgcgca tcaccccggc ggggaagttt
      601 ctgcgaaaat ggaaactcga tgaattgcca caattgtgga acgtgtttcg cggcgacatg
      661 agtctggtcg gtccccgtcc ggaagttgaa cgctatgtcg ccctgtacac agcggaacaa
      721 cgggaagtgc tgcgcgtaag accgggcatc accgacaaag cttccatcgt ctatgctgat
      781 gaaaacgaac gactcagcaa ggccggagat cccgaagctc attaccggga attcatcctg
      841 ccggacaaga tccgcttgaa tatggaattc atccgcaaac cgacttttgg ccattatctc
      901 cggatcctgt ggatgacttt tacgcatctc ttttttcgat catccgccgg tcagtagagc
      961 cttcattatc agatgaaaaa caaccacccg cggaaacaaa atgccgatgg cagtagttta
     1021 aggatgcaga atctgggatg aattcagtgt aattcttcca ttttccgctt aaaaacgtta
     1081 aatttgcatt ccaccggaat tgtccggatt ttctaaaggg aaatttttta ccacctatac
     1141 aatgcgtatc aagatctctt ttctcaaggt gcatggaagc agtggtaccg ttccgcttca
     1201 ccaccaaaag atcatctccg ctttcatgga tgaagtgatt cgtgaaatgc cgatcagttc
     1261 gcccttctac aatttctctt ccctgaaggg aacatcgaaa gtgcagaacg gacagatccg
     1321 gttcctgtct tccaaagtct cgctggtgct atccgcaccc gacaaagagt ttgcggaaga
     1381 atgggtgcgc cggatatttg agcgacgcct cgtgtcgttc gctaaactga ccctggtacc
     1441 caaggcctcg gaggtcatcc cggatcctga cttcaaccag gtgatgaagt acgtgtgtat
     1501 ttcgcccatg gttccgcagg gaccgttcga gagcgacccg gagggaaaca ttccggatcc
     1561 tttggatccc agggagcagg acttctccga tgctttctat gatgtactga tggaaaacat
     1621 ggagcgggcc ggattcacgc cgcagcaact gcatgacttc gccgaattcg agatcacgcc
     1681 ggacccggga tacattcaga agatcctgaa tacgcacaag aagttcgcgc gtatctataa
     1741 gaacaacgac agtttgccga tc
//
LOCUS       DAOTON010000655         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_52543_length_1761_cov_2.885698, whole genome shotgun sequence.
ACCESSION   DAOTON010000655 DAOTON010000000
VERSION     DAOTON010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52543_length_1761_cov_2.885698"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="P5292_11415"
     CDS             complement(<1..1553)
                     /locus_tag="P5292_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS39779.1"
                     /translation="MRTILGLMAACLFGIPASAQQTLSNDTTNKEKDLPELIISANRT
                     LQNRMSIPQQVAAIPASKIRFGNFPTMAEVLQNSGTLFVQKSQQGGGSPVIRGFEASR
                     VLLVIDDVRMNNLIYRAGHLQNVITVDPNILDRVEVLYGPSSTVYGSDALGGVVHLRT
                     RKPEFSTDGTLLAKGSGMMRYASANRERTVQANVNLGKSKFASLTSITYSMFDDLRMG
                     KQAGSLDSVWGKRYYYVERINGKDSLVKNDDPYVQKFSGYDQLDLMQKFSLKTGEHTV
                     HGLNLQYSTSTDIPRYDRLTDPNASTTLNSAEWYYGPQDRLLAAYHYDRKLTRGFFDA
                     VRAVLSYQYVVESRHNRGFGGSNRTNRVEYVNVAGYTLGLDRNRGPHQLRLGIDGQYS
                     AVNSVANRYNVNTGALSAQSTRYPDGDNSMNSIAAYVTHTWEVTPKFSVHDGLRFTSL
                     RLESSFEDTSFFPFPVSGVTQENTALAGNLGVVWRPDLHVKVSLLGTTGFRHRMWMTW
                     PRYSNQRPV"
BASE COUNT          377 a          472 c          513 g          399 t
ORIGIN      
        1 ctcaccgggc gttgattcga ataccttggc caggtcatcc acattcggtg ccggaaaccc
       61 gtggttccca gcaacgacac cttgacgtgc aggtcggggc gccatacgac tcccaggttg
      121 ccggccagcg cggtattctc ctgcgttaca ccgctgaccg ggaacgggaa gaaactggta
      181 tcctcgaatg aactctccaa acgcaaactg gtgaaccgta gtccgtcgtg cacggaaaac
      241 ttcggggtca cctcccaggt atgtgtgaca taggccgcga ttgagttcat ggaattgtcg
      301 ccgtcaggat aacgcgtgct ttgcgcagac aacgcacccg tgttcacgtt gtagcggttg
      361 gcaacggagt tgaccgcact gtactgtcca tcgataccga gccgcagttg gtgcggtccg
      421 cggttgcgat cgagtccaag cgtataaccc gcgacattca cgtactccac ccggttggtc
      481 cggttggatc cgccaaagcc gcggttgtgg cggctttcga cgacatactg gtaactcaaa
      541 acggcgcgca cggcatcgaa gaaacctcgg gtaagtttcc ggtcgtaatg ataggcggcc
      601 aggaggcgat cttgcggacc gtaataccac tcagcgctgt tcaaggtcgt ggaagcgtta
      661 ggatccgtca ggcggtcgta gcgtggaata tcggtggagg tactgtactg cagattgaga
      721 ccgtgcacgg tatgttcgcc tgtcttcaag ctgaacttct gcatcaggtc caactggtcg
      781 tacccgctga atttctgcac atacgggtcg tcgtttttaa cgagtgaatc tttcccgttg
      841 atccgttcca catagtaata ccgctttccc cagacggagt ccagcgaacc ggcttgcttg
      901 cccatccgca ggtcatcgaa catgctatag gtgatggagg tcagggatgc gaacttactt
      961 ttacccagat ttacattcgc ctgaacggtc cgttcccggt tggctgaagc atagcgcatc
     1021 atgcccgaac ctttggccag caaggttccg tcggtggaga attccggctt gcgggtacgc
     1081 aggtggacga caccacccag tgcatcgctg ccataaacgg ttgaggatgg tccgtacaat
     1141 acctcgacgc gatccaggat gttcggatca acggtgatga cattctgcaa gtggccggcc
     1201 cggtagatca ggttgttcat ccggacgtcg tcgatgacca acagcacacg gctggcttcg
     1261 aagccgcgga tcaccggact tcctcctccc tgctggctct tttggacgaa gagggtgccg
     1321 gaattctgca acacctcggc catggtcggg aagttgccga accggatctt ggaagccgga
     1381 atggcggcga cctgctgcgg aatgctcatg cgattctgca gggtacggtt ggcagaaatg
     1441 atcagttccg gaaggtcttt ttctttattg gtagtatcgt tcgatagggt ttgttgtgct
     1501 gaggccggga tgccgaatag gcacgccgcc atcagcccta ggatggtacg cattgatgat
     1561 tggtttggaa agatgaatat tcgatcagcc tccgcgcagg caggccgtag ctaaaaggaa
     1621 accattaacc aaacatcaat gggggtggca ccatcgggct aacgacgggc tcgggtagtc
     1681 cttgaaaggg ttccgcgaac gccagttcca gacaaaactg tttattccag gcgggtgttt
     1741 caatcgattc caggtatacc g
//
LOCUS       DAOTON010000656         1752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_52899_length_1752_cov_4.180907, whole genome shotgun sequence.
ACCESSION   DAOTON010000656 DAOTON010000000
VERSION     DAOTON010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52899_length_1752_cov_4.180907"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="P5292_11420"
     CDS             complement(<1..1158)
                     /locus_tag="P5292_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39780.1"
                     /translation="MTTFTLAHRPLTRLLSGLLFCLFLFGQTERATAQGQSFPCVLKC
                     RDTTICFNLPDSMVQLLPPAYAPAGPVIGNGPDCQFDSIWSNAPGVYPVGTTVVTWYV
                     WAPPGRLDSCTMNVVRNPPSSYTISFTTSPPIVGGVINICNGQSITFNDNSTGISGLL
                     WNFGNGYYSSNATHTEPAWHYPPGTYYDTLTVYDDCGSPHDTAFMVVVDSASGPDITC
                     ISVQCPGDTVTYHTSAVCTNYTWAVTGGTFLTPPPPTSDSCTVIWGNGPSGTISLSVN
                     GCTPPLNCPNPTIKTVSIVPATLPIAGDTIVCSGSTSTYCVECIPGNIHNWELMPANA
                     GTISGQGTCCLTIQWAPGFAGTVTLTLNYQNILTGSGCNLPGNCRDDHNCGG"
     gene            complement(1200..1397)
                     /locus_tag="P5292_11425"
     CDS             complement(1200..1397)
                     /locus_tag="P5292_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRS39781.1"
                     /translation="MRRKFSDAFKQQVLAEHVQGRLSLSEIARKYDIEVRLLKSWMNE
                     RAASLNGRESGIPMTGEHLQK"
     gene            complement(1537..>1752)
                     /locus_tag="P5292_11430"
     CDS             complement(1537..>1752)
                     /locus_tag="P5292_11430"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRS39782.1"
                     /translation="GTRNIKVYEQLLSEQVFFRAHHSHLINLRHVRNFSRVDGYFVEM
                     ADGSRVEISRRKKDEFLERMSYAGGKA"
BASE COUNT          392 a          484 c          501 g          375 t
ORIGIN      
        1 accgccgcag ttgtgatcgt cgcgacagtt gcccggcaga ttacaacccg atccggtgag
       61 gatgttctgg tagttcaggg tcaaggtcac tgttccggca aatcccggcg cccactggat
      121 cgtcaggcaa caggtcccct gcccgctgat ggtcccggcg tttgccggca tcagttccca
      181 gttgtgaata ttacccggga tgcattcgac acaatacgtc gaggtggaac cggaacagac
      241 gatcgtatca cccgcgattg gcaatgtcgc gggaacgatg ctgacggttt tgatcgtcgg
      301 gttcgggcag ttgagcggcg gcgtacaacc gttcacgctg agcgagatcg tacccgaagg
      361 accattgccc cagatcaccg tacaggaatc cgatgtggga ggcggtggtg tcaggaaggt
      421 gccgcccgtc accgcccagg tataattggt acacaccgcg ctggtgtgat aggtaaccgt
      481 gtcgcccgga cactgtaccg aaatgcaggt gatatccgga cccgaagccg aatccaccac
      541 caccatgaag gccgtatcgt gcggtgaacc gcaatcgtcg tagaccgtca gggtatcgta
      601 atacgttccg ggcggataat gccaggccgg ttcggtatgc gtcgcgttgc tgctgtaata
      661 accattcccg aagttccaca acaaaccact gataccggtg gagttgtcgt tgaaggtgat
      721 cgattgtccg ttacagatgt tgatcacgcc accgacgatc ggcggactgg tcgtaaaact
      781 gatcgtatac gaggatggtg gattgcgcac cacgttcatc gtgcaggaat ccagtcgtcc
      841 gggaggagcc cagacatacc aggtcacgac cgtagtaccg acgggataca cgcccggcgc
      901 attgctccag atggaatcga actggcaatc gggtccattg ccgatcaccg gtcccgccgg
      961 tgcgtaggcg ggtggcagca attgcaccat cgaatccggc aggttgaaac agatggtcgt
     1021 atcccggcat ttcaacacgc agggaaaact ctgcccctgc gcggtcgccc gctccgtctg
     1081 gccaaacaga aacaggcaga aaagcagacc cgaaaggagt cgcgtcaacg ggcgatgtgc
     1141 aagcgtaaag gtggtcatga tgtgcgtgtg tttcgttgga cagttaactt gactccgggt
     1201 catttttgta aatgctctcc ggtcatgggt attcctgatt cgcgcccgtt cagggaagca
     1261 gcgcgctcgt tcatccagga cttcagcaac cggacttcaa tatcatattt ccgggcaatt
     1321 tcgcttaacg acaaccggcc ttgcacgtgt tccgcaagta cctgttgctt gaaggcgtca
     1381 ctgaatttcc ggcgcatgga gctggtgttt gcctcggcaa atgcccgatt atgttggaaa
     1441 aatggaaact tttggcagat tgaatgcacc ggattaaccc gcggcgggta aatgcaacaa
     1501 ctggctgatc aggtttaacc cccggcagat ccggcatcag gctttcccgc ccgcgtaact
     1561 catacgctcc aggaattcgt ccttttttct gcgggagatt tcgacccgcg acccgtcagc
     1621 catctcgaca aaatagccgt cgacccgact gaaattccgg acatgccgca gattgatcag
     1681 gtgggaatgg tgcgcgcgaa aaaaaacctg ctcgctcagg agttgctcat acactttgat
     1741 gttacgcgta cc
//
LOCUS       DAOTON010000657         1752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_52900_length_1752_cov_3.877431, whole genome shotgun sequence.
ACCESSION   DAOTON010000657 DAOTON010000000
VERSION     DAOTON010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52900_length_1752_cov_3.877431"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="P5292_11435"
     CDS             <1..166
                     /locus_tag="P5292_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRS39783.1"
                     /translation="RKEFSYSSFRRSFTMPEHVDAEQIQAEYNNGVLSINLPKRVEEK
                     KKAGREITVA"
     gene            289..1080
                     /gene="surE"
                     /locus_tag="P5292_11440"
     CDS             289..1080
                     /gene="surE"
                     /locus_tag="P5292_11440"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRS39784.1"
                     /translation="MGNGRPLILVTNDDGITAPGLHALVNAMSALGDLYVVAPDSPQS
                     GMGHAITIHNMLRLDKVEVHGDHDWYQCSGTPVDCVKIAMDKILPRKPDLCVSGINHG
                     SNSSINVIYSGTMSAAMEGAIEGIPSVGFSLLNYSLDADFSGAAHVARIVASRVLEKG
                     LPQGSLLNVNIPSLPLEDLKGIKICRQAMAKWEEEFDERIDPTRRKYYWLTGKFVNHD
                     RGEDTDEWALANGYVSVVPVQFDFTAHHAIPFLNEQGYELNKIAR"
     gene            1055..1360
                     /locus_tag="P5292_11445"
     CDS             1055..1360
                     /locus_tag="P5292_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39785.1"
                     /translation="MSSIRLPADSFLAGALSALIFLPLLNYLLGHLGSWLTEQTGNDR
                     WSIAPHPQLFTLLVAAIVFRLLVINYKREHTGRGWLMVTFLTAMAYAFWYYRFRHAG"
     gene            1350..1652
                     /locus_tag="P5292_11450"
     CDS             1350..1652
                     /locus_tag="P5292_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39786.1"
                     /translation="MQAETRKRLDILYGGLLAGLVLPFLAFAFYTSLIQNAGVVEMIQ
                     RYYERHVLSHVISLSLIANLGLFFLCLRFNLEKTARGVLFSMFIYGGLIVLLKFVL"
BASE COUNT          363 a          461 c          486 g          442 t
ORIGIN      
        1 gcgtaaggag ttttcctact cctctttccg ccgcagcttt accatgcccg agcatgttga
       61 cgccgaacag atccaggcgg aatacaacaa cggagtgctt tccatcaacc tgcccaagcg
      121 cgtggaagag aagaaaaaag ccggccgtga gatcacggtg gcctgagtgt aaatttctgt
      181 gttgagtagt gggttagcgt cgccggtatg gtccggcggc gcttctttgt tctgtagccc
      241 ggccctcgct ttttactacc tttgcggcat tcaaacgcat ttgctgttat gggtaatgga
      301 cgtccgctga tcctggtcac caatgacgac ggtatcaccg ctcccggttt gcatgcattg
      361 gtcaacgcca tgagtgcact gggagatctg tatgtggtgg cgccggatag cccccagtcg
      421 ggcatgggcc acgccattac gatccacaac atgcttcgtt tggacaaggt ggaggtgcac
      481 ggcgatcatg actggtatca atgcagcgga acgccggtgg actgtgtgaa gatcgcgatg
      541 gacaagatac ttccccggaa accggatcta tgcgtcagcg gtatcaacca cggaagcaac
      601 tcctcgatca atgtaattta ttccggtacc atgtcggccg cgatggaagg cgctatcgaa
      661 ggcattccct cggttggatt ttctctcttg aactattccc tcgatgccga tttctccggc
      721 gccgctcacg tagcgcggat cgtcgcttcc cgtgtgctgg agaaaggctt gccgcaggga
      781 tctttgttga atgtcaacat tccgagtttg ccgcttgagg atttgaaagg gatcaagatc
      841 tgccggcagg caatggcaaa gtgggaggag gagttcgacg aacggatcga tccgaccagg
      901 cgcaagtact attggctaac cggaaagttt gtcaatcatg accggggaga ggataccgat
      961 gaatgggcgc tcgccaacgg ttatgtgtcg gtggttcccg tacagttcga ctttaccgcc
     1021 catcacgcga ttcctttcct gaatgaacag ggatatgagc tcaataagat tgcccgctga
     1081 ttcgttcctg gcgggagcct tatcagcgct catcttcttg ccgctgctga attacctcct
     1141 cggtcatctg ggtagctggc tgacggagca gactggaaat gatcgctggt cgatcgcccc
     1201 gcatccgcaa ctctttacct tactcgtagc ggctatcgtc tttcgactcc tggtgatcaa
     1261 ttacaaacgg gagcacaccg gtcgcggatg gttgatggtt accttcctga cagccatggc
     1321 ttacgctttt tggtactacc gattccgaca tgcaggctga aacgagaaaa cgactggata
     1381 ttttatacgg cggactcctt gccgggctcg tgttgccgtt cctggccttc gcgttctaca
     1441 cttcgctgat tcagaacgcc ggtgtggtgg agatgatcca gcgatactat gagcgacacg
     1501 tattgtccca cgtcataagt ttgagcctga tcgcgaacct cggtctgttc ttcctctgtc
     1561 tgcgattcaa cctggaaaag actgctcggg gtgtcctgtt ttccatgttc atctacggtg
     1621 gtttgatcgt cctcctgaaa ttcgtcctat gaagtattac atcatttcag gagaagcatc
     1681 cggcgatctg cacgccgcca acctggtgcg cgaactgcga aagctggatg ccggagcgga
     1741 ttttcgcgga tg
//
LOCUS       DAOTON010000658         1751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_52953_length_1751_cov_3.058373, whole genome shotgun sequence.
ACCESSION   DAOTON010000658 DAOTON010000000
VERSION     DAOTON010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52953_length_1751_cov_3.058373"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..>1751
                     /locus_tag="P5292_11455"
     CDS             227..>1751
                     /locus_tag="P5292_11455"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HRS39787.1"
                     /translation="MKNYLRIGSLLTLLLLQLTVSAQQRVRCFTEEHKTYLRTKQPKL
                     DEQLRQARAKAEQWAAAHPDWRNESGGLMTIPVVVHVVYNTTGQNISDAQVESQIDVL
                     NEDFARLNADTANTPAPFASIAGPTPIRFCLAQRTPDDQPSTGIERRQTGTSSFFDDD
                     AIKAFSSGGLDAWDPTRFFNIWVGPLGGGLLGYAEFPSGSISNTYGVVIAYDAFGRIG
                     TAASPFDLGRTASHEIGHCFGLYHIWGDDGNSCSGSDGISDTPNQGGATFGCRTFPAT
                     DACTSTGNGVMFMNYMDYSDDNCLNIFTEEQATVMVGTINSFYSSLLSSDGCTPVNIA
                     ALDAGINAITSPTGAFCDTTITPVVTLRNYGANALTSVNFNYSIDGGTVSTYAWTGNL
                     ASLTSVAVNLPAISVTGGPHTFTCYTSDPNSGTDANAANDSQSSNFNVNTLGASLPVV
                     EGFEGTFPPVNFTIENPDAATTWERSTAASHSGSAVSFLDNYNYNAAGQRDDLVLPAF
                     "
BASE COUNT          375 a          544 c          447 g          385 t
ORIGIN      
        1 cgttgcggat ttgcattttc ctttgaaatt ttgctaaaaa gcgggaaagg gcttttccct
       61 cgccctccct tcagcgggcc gcgaaggtac ggacttaaat ccgaagaaga tagcctgtcg
      121 tgacatttcc ccggttaacc gatctctggc ccggaacagg agctttttca tttaccgcgc
      181 tggcggactt tactactttc ggcccggcca aaccggctaa accatcatga aaaactacct
      241 gcgcatcggg tctctcctga ccctgctgct tctgcagcta accgtttccg cccagcaacg
      301 cgtccgttgc tttacggaag aacacaaaac ttacctccgg acgaaacaac ccaagttgga
      361 cgagcaactg cgacaagccc gagccaaagc ggaacaatgg gccgcggcgc atcctgattg
      421 gcgaaacgaa tccggcggcc tgatgaccat cccggttgtc gtgcacgtgg tgtataatac
      481 caccggacag aacatcagtg atgcgcaggt ggaatcccag atcgatgtgc tcaacgaaga
      541 tttcgcgcgc ctgaatgccg atactgccaa cacacctgca cccttcgctt ccattgccgg
      601 gcctacgccg atccgcttct gcctcgcgca gcgtacgccg gacgaccagc cctctaccgg
      661 cattgagcgc cggcagaccg gtacgagttc gttcttcgac gacgacgcca tcaaggcatt
      721 cagttccggc ggactcgatg catgggatcc gacccgtttc ttcaacatct gggttggccc
      781 actgggtggc ggtttgctgg gttatgccga gttccccagt ggaagcatca gcaacaccta
      841 tggcgtcgtg attgcctatg acgctttcgg tcgtatcgga acagcggctt ctcctttcga
      901 tcttggccgt accgcgagcc acgagatcgg tcattgcttt ggtctctacc atatctgggg
      961 ggatgatggc aactcgtgta gcggcagtga cggcatcagc gataccccca accagggcgg
     1021 cgcaaccttc ggttgcagaa ctttcccggc taccgacgcc tgtacttcga ccggcaacgg
     1081 cgtgatgttc atgaactata tggattacag tgacgacaac tgcctgaaca tctttaccga
     1141 ggagcaagcc accgtgatgg tcgggacgat caacagcttc tattcttccc tgctatcttc
     1201 tgatggttgt acgcccgtca atatcgcggc gctcgacgcg gggatcaacg ccattacttc
     1261 tccaacgggc gctttctgcg acaccacgat cactccggtc gtgacgctgc gtaattacgg
     1321 cgccaacgcg ttgaccagcg tcaacttcaa ctattccatc gacggcggaa cggtgagcac
     1381 ttatgcctgg acgggcaacc tggccagtct gacctctgtg gctgtcaacc tgccggcaat
     1441 cagcgtaacc ggcggacctc acactttcac ctgttatacc agtgatccga acagcggaac
     1501 cgatgccaat gccgccaatg attcgcaatc atccaatttc aacgtgaaca cactgggcgc
     1561 ttcgctgccg gtcgtagaag gatttgaagg cacgttcccg cccgtcaatt tcacgatcga
     1621 gaatccggat gccgctacta cctgggaacg ttccacagca gcttcccata gcggcagtgc
     1681 cgtctctttc ctggacaact acaactataa tgcagccggc caacgcgacg atcttgtcct
     1741 acccgccttc g
//
LOCUS       DAOTON010000659         1746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_53172_length_1746_cov_3.746895, whole genome shotgun sequence.
ACCESSION   DAOTON010000659 DAOTON010000000
VERSION     DAOTON010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53172_length_1746_cov_3.746895"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1296)
                     /locus_tag="P5292_11460"
     CDS             complement(13..1296)
                     /locus_tag="P5292_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRS39788.1"
                     /translation="MSPLAERMRPEDLDHYIGQEHLVGPGGVLRRSIEANTIPSMILW
                     GPPGVGKTTLAGIIAKKLRRPFYILSAVSSGVKDVREVIEKASQYSRELKEVPGNAIL
                     FIDEIHRFSKSQQDSLLAAVEKGIITLIGATTENPSFEVISPLLSRCQVYVLRELTRD
                     ALLAMLQQAIERDDYLRQRKVELKESDALLRISGGDGRKLLNLLELVVQSMPPSSPAI
                     ITDEAVLSVAQQNLARYDKGGEQHYDIVSAFIKSMRGSDPNAAVYWLARMIAGGEDPS
                     FIARRMLILASEDIGMANPTALVIANACFEAVNRIGYPECRIILSQAAVYLATSPKSN
                     ASYMAIEEALALVNKTGDLPVPLHIRNAPTRLMKELEYGKGYAYDHSRQGSFSGQEFL
                     PSEISGTRLYDPGKNSRENELREWLKVRWKDKYDY"
     gene            complement(1318..>1746)
                     /locus_tag="P5292_11465"
     CDS             complement(1318..>1746)
                     /locus_tag="P5292_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39789.1"
                     /translation="TFSNQQFEDNVVSDISITSPMGTQWIAGIKSFIQDRGSNIRSQL
                     SSLGCIVGVDHSTAGNSKPQLFPNPANSTTFIRWEQPLANPTRVEMLDLSGKMIRISQ
                     VQVGVSEYVLSLDDLPAGVYLLRLGSEGTAPTCLPLMITR"
BASE COUNT          400 a          475 c          473 g          398 t
ORIGIN      
        1 ggattcacct gctcagtagt cgtatttatc cttccagcga actttcaacc attcccgaag
       61 ttcgttttcc cggctgttct tccccggatc atacaggcgt gtaccgctga tctccgaagg
      121 caggaactct tgcccggaaa aactgccctg cctggaatga tcataagcat agcctttacc
      181 gtattccagt tccttcatga gccgggtagg ggcgttgcgg atgtgcaaag ggaccggcag
      241 gtccccggtt ttgttgacca gtgcaagggc ttcttcaatg gccatgtagg aggcattgct
      301 cttgggggag gtggccaggt aaacggccgc ctgagaaagg atgatcctgc attccgggta
      361 gccgatgcga ttcactgctt cgaagcaggc attggcgatc accaaagcgg ttggattagc
      421 cattccgatg tcctccgaag ccaatatcag catcctacgc gcgatgaagg acgggtcttc
      481 accgcctgcg atcatgcgcg ccaaccaata cacggcggca ttcgggtcgc tgccccgcat
      541 ggatttgatg aacgcactga cgatgtcata atgctgctcg ccccctttgt cgtatcgagc
      601 cagattctgc tgcgcaacgg agagcactgc ttcgtcggtg atgatcgctg gcgaggacgg
      661 cggcatggat tgtaccacaa gctccagcaa attcaacaat ttgcgaccat cgcctcctga
      721 aattcgcagt aaggcatcgg actctttcag ctcgacttta cgttggcgca ggtagtcgtc
      781 gcgctcgatc gcctgctgca acatggccag caacgcatcg cgcgtcagct cccgcaaaac
      841 gtaaacctga cagcggctga gcaagggaga aatcacctcg aaggatgggt tctccgttgt
      901 ggcgccgatc agcgtaatga tacccttctc cacggctgcc aacaacgagt cctgttggga
      961 cttgctgaac cggtgaattt catcgatgaa aaggatcgca ttaccgggaa cctctttcaa
     1021 ctccctcgaa tattgggaag ctttttcgat gacttccctg acatccttga cacccgacga
     1081 aacggcactt aggatataaa acggccggcg gagctttttc gcgatgatac ccgccaaagt
     1141 cgttttaccg actccgggag gcccccacaa aatcatggac gggatggtat tggcttcgat
     1201 ggaacgacgc aacacacctc ctggacctac taagtgttcc tgtccaatgt aatggtccag
     1261 atcctctgga cgcatgcgct cggcgagcgg actcatgccg caaaggtaag caaatgctca
     1321 gcgtgtgatc atcaacggca ggcaggtcgg agccgtcccc tccgaaccca gccggaggag
     1381 gtaaactcca gccggaagat cgtccagcga aagaacatat tccgaaactc caacttgaac
     1441 ctgagaaatt cggatcattt taccggacag gtcaagcatt tccacacgtg ttgggtttgc
     1501 caaaggttgt tcccagcgaa tgaaggtggt gctattggca ggattgggga ataattgagg
     1561 tttggaattt cccgccgtgg aatgatcgac tccaactatg cagcctaagg aggacaattg
     1621 gctccggatg ttgcttcccc ggtcctggat gaacgacttg atgccggcga tccattgtgt
     1681 acccatgggg ctggtaatgc tgatgtcgga aaccacgttg tcctcgaact gttgattgga
     1741 gaaggt
//
LOCUS       DAOTON010000660         1744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_53273_length_1744_cov_2.789816, whole genome shotgun sequence.
ACCESSION   DAOTON010000660 DAOTON010000000
VERSION     DAOTON010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53273_length_1744_cov_2.789816"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1744
                     /locus_tag="P5292_11470"
     CDS             <1..>1744
                     /locus_tag="P5292_11470"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS39790.1"
                     /translation="YCRGAAINAGGGGNGHNAGGGGGGNAGNLAAWTGSGNPDVSNAA
                     WAAAWNLEYSGFAGSTSSGGGRGGYTFSSSDQNALSLGPFNSSWGGDQRRNNGGMGGR
                     PLDYSTGRIFLGGGGGSGDQNDSKGGPGGNGGGLAYLFSFGDVSGAGEIRANGSNGVS
                     TSTSNGTDGAGGGGAGGTVILKAGGTVSGVSISANGGNGGNQVVNILNLEAEGPGGGG
                     GGGYIGIGSGAPTCSANGGANGTTNSRSMTEFPPNGATRGGAGLPNESVPAYKFIYTP
                     LVICPGNTATINVVTSGTPPATISYQWYTQAVGGVPTGFSGVSYTTPPLFATDTFYAA
                     VCPGHYRYPFIVQVDAMTASFTPPSGCTGQSLSFSGTGTSSMSTISGYSWNFGDGSPA
                     SNSQNTTHTYSSSGNYTVTLTVTNASGCTMTSTQTVAVSAPPVISVSPSVTSGCGPLS
                     VNFSNSTTGATGYTWNFGDGSPTSSSTSPTHVYTSNGTYTVTMTATNASGCSATYTGT
                     NLISVANGPTASFSNATPSICLGDTIRFTNLSTANGTPISGYSWDFDDGTPASTQTSP
                     THIYTSPGTYAVTLT"
BASE COUNT          349 a          528 c          520 g          347 t
ORIGIN      
        1 gttactgtcg tggcgcggcg atcaacgccg gtggcggcgg gaacggacac aatgctggcg
       61 gcggcggcgg cggtaatgcc ggcaaccttg cagcctggac cggcagcgga aatccggatg
      121 tttcgaacgc cgcctgggcg gcagcctgga atctcgagta tagcggcttt gccggttcta
      181 catcttccgg tggaggtcgc ggaggttata cgtttagcag ctccgaccag aatgcgcttt
      241 cactcggacc cttcaatagt tcctggggcg gcgaccagcg tcgaaacaat ggcggcatgg
      301 gtggtcgtcc gctggattac tcgaccggca ggattttcct cggcggcggc ggcggctctg
      361 gcgatcagaa cgattcaaag ggaggtccgg gcggtaatgg cggcggactc gcgtatttat
      421 tttcattcgg agatgtcagc ggcgccggag agatccgcgc caacggcagt aatggcgtga
      481 gcacgtcgac gagcaatggt accgatggtg ccggcggcgg cggcgcgggc ggtaccgtta
      541 tattgaaggc cggaggaacc gtatcgggcg tatcgatttc cgcgaatggt ggcaatggcg
      601 gcaaccaggt cgtgaacatc ctgaatctcg aagcggaagg tccgggtggt ggcggcggcg
      661 gcggttatat cggtattgga tccggagcgc cgacgtgcag cgcgaatggc ggagcgaacg
      721 gtacgaccaa ttcccgcagc atgaccgagt ttccgccgaa cggtgcgacc aggggcggcg
      781 ccggcttgcc caacgaaagc gttcccgctt acaagttcat ttatactccg ctggttattt
      841 gtccgggcaa cacagctacg atcaatgtcg tcaccagcgg tacgccgccg gcaaccattt
      901 cctaccaatg gtacacacaa gccgttggcg gagtgccgac gggtttcagc ggcgtttcct
      961 ataccactcc gccgctcttt gcaaccgaca ccttctatgc cgccgtttgt ccggggcatt
     1021 atcgttatcc gttcatcgta caggtggatg cgatgacggc ctcgttcacg cccccctcgg
     1081 gttgtaccgg acaatcgctg agcttttccg gtaccggaac aagttccatg agcaccatca
     1141 gcggttacag ctggaacttc ggtgacggaa gtccggcgag taattcgcag aacacgacgc
     1201 acacctattc gtcatccgga aactatacgg taacgctgac tgtcacgaac gccagcggtt
     1261 gcacgatgac ctcgacgcag accgtcgctg tctccgcacc gccggtcatt tccgtttctc
     1321 catcagtaac ctccggttgc ggtccattgt ccgtcaattt ctccaacagc acgaccggcg
     1381 ccaccggtta tacctggaac tttggcgacg gatcaccgac atcgtcttcc accagtccga
     1441 cgcacgttta taccagtaac ggaacctaca cggtgacgat gacggccacc aacgcttcgg
     1501 gttgcagcgc gacgtatacc ggcacgaacc tgatcagtgt agccaacggg ccgaccgcat
     1561 ccttcagcaa cgcaactccg tcgatttgtc tgggcgacac gattcgcttt acaaatctgt
     1621 cgaccgccaa cggcacgccg atctccggct atagctggga tttcgacgac ggcactcccg
     1681 cctcaacgca aaccagtcca acgcacattt acacgagtcc cgggacctac gccgtcacgc
     1741 tgac
//
LOCUS       DAOTON010000661         1742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_53351_length_1742_cov_3.439834, whole genome shotgun sequence.
ACCESSION   DAOTON010000661 DAOTON010000000
VERSION     DAOTON010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53351_length_1742_cov_3.439834"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="P5292_11475"
     CDS             complement(<1..409)
                     /locus_tag="P5292_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HRS39791.1"
                     /translation="MKKVVKDADAAIEGIQDGMTLLVGGFGLCGIPENCIAALVRKGV
                     KGLTCISNNAGVDDFGLGLLLQHRQIRKMISSYVGENAEFERQMLSGELEVELIPQGT
                     LATRCLAAGYGMPAVFTPAGVGTEVAEGKETRTF"
     gene            complement(456..>1742)
                     /locus_tag="P5292_11480"
     CDS             complement(456..>1742)
                     /locus_tag="P5292_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRS39792.1"
                     /translation="YTTIDSRMQRYAEEALREHLTEKQKQFFEHWKGKEPWKEHPEVV
                     TEGMKRSDRYITMKAAGASDKEIQKAFNKKTEMTVFSWKGPIDTVMTPLDSVKYHKKI
                     LQGGFMAMEPQTGYVRAWVGGNDYRFFKYDHVKAGRRQVGSTFKPFLYTLAMQEGYSP
                     CYKVPNARVYFDLPTGETWSPENADAKYGGMLTLKEGLAESVNCVSAYLMKQFGPQAM
                     IEICRKMGISAPIDAVPAICLGTPDVSVYEMVGAYCSFANKGVWTEPTYITRIEDKNG
                     NILQEFVPRKVEAINEETAYLMLNLMQGVVQFGTGARLRGQYKLPYPIAGKTGTTQNQ
                     SDGWFMGITPDLAAGCWVGCEDRIVHFRTLELGQGARTAMPIWALFMQKVYADANIKI
                     SKGEFEAPKEKLSVELNCDRYVQQGGSSGENPDKDF"
BASE COUNT          329 a          502 c          469 g          442 t
ORIGIN      
        1 tgaaggtgcg ggtttctttt ccttccgcga cctcggtgcc tactccggcg ggcgtgaaca
       61 ccgcgggcat gccgtatcct gcggcaagac aacgggtagc caacgtaccc tggggaatca
      121 attccacttc cagctcacca ctcagcatct gacgttcaaa ctccgcgttc tctcctacgt
      181 aggacgagat cattttcctg atctgacgat gctgcaggag tagccccagg ccgaaatcat
      241 cgactcctgc attattagaa atacaggtca gcccttttac gcctttgcgt accagcgcgg
      301 caatgcaatt ctccggtatg ccgcagagtc cgaagcctcc caccagcagg gtcataccat
      361 cctggatgcc ttcaatggcg gcgtccgcgt ctttgacgac tttcttcata tcgcaggaaa
      421 tctttgacga aattaagcaa ccggcggcat tccgcttaga aatccttgtc gggattttct
      481 ccggaactac cgccctgctg cacgtacctg tcgcaattga gctcaacgct cagcttctct
      541 ttgggcgctt caaattcgcc cttggagatc ttgatgttgg catctgcata gactttttgc
      601 atgaacaagg cccagatggg catggcggta cgggcgccct gcccgagttc cagtgtccgg
      661 aagtgaacaa tccggtcctc gcagccgacc cagcaacctg cggccaggtc gggcgtgatg
      721 cccatgaacc agccgtcgct ctgattctgc gtcgttccgg ttttaccggc aatcgggtag
      781 ggtagtttgt attgtccgcg cagccgcgct ccggtgccga attgtaccac gccctgcatc
      841 aggttgagca tcaggtaggc ggtctcttca ttgatggctt ccactttgcg cggcacgaat
      901 tcctgcagga tgttgccgtt cttgtcttcg atgcgcgtga tgtaggtcgg ctccgtccag
      961 acgcctttgt tcgcgaagct gcagtaggcg ccgaccattt cgtacacgga tacgtcaggt
     1021 gtacccaggc agatcgcagg taccgcgtcg atcggggcgg agatacccat cttccggcag
     1081 atctcgatca tggcctgagg accgaattgc ttcatcaggt aagccgatac gcagttcacc
     1141 gattccgcca gtccttcttt cagggtcagc atgcctccgt atttggcatc ggcgttttcg
     1201 ggcgaccagg tttcaccggt cggaagatcg aaatagacgc gtgcgttcgg tactttgtaa
     1261 cagggtgaat agccttcctg catcgccagc gtgtagagga aaggcttgaa ggtcgaaccg
     1321 acctggcgac gacccgcttt tacgtggtcg tacttgaaga aacgataatc gttgccgccc
     1381 acccaggccc gtacataacc ggtctgcggt tccatggcca tgaatccacc ctggaggatc
     1441 ttcttgtgat atttaaccga atccagcggg gtcatgacgg tgtcgatcgg acctttccag
     1501 gaaaagacgg tcatctccgt tttcttgttg aaggctttct ggatctcctt atccgatgcg
     1561 ccggcagcct tcatggtgat gtagcggtcg gagcgtttca ttccttccgt tacgacttcg
     1621 ggatgttcct tccagggttc tttgcctttc cagtgttcga agaattgctt ttgcttctcc
     1681 gtcaggtgct cgcgcagggc ttcttcggca taccgttgca tccgcgaatc gatggtggtg
     1741 ta
//
LOCUS       DAOTON010000662         1735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_53713_length_1735_cov_2.725595, whole genome shotgun sequence.
ACCESSION   DAOTON010000662 DAOTON010000000
VERSION     DAOTON010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53713_length_1735_cov_2.725595"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..>1735
                     /locus_tag="P5292_11485"
     CDS             192..>1735
                     /locus_tag="P5292_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39793.1"
                     /translation="MRHYLIALFCLGAFILFVSSIPDDEPRPRFHSPAELEALQQRVH
                     SPLVPGEWYMTSASCRGCHGHDTLGLANINENGEDVNLFSHWESSMMALSAKDPLWRA
                     KVSQEILVNPGHAAELQDKCTSCHAPMGRYNSRFRGNAHYGLTDLYADSLGLDGVSCA
                     GCHTIDSTVGLTFSGEIPYDTTRTIYGPFTFPMTGPMQLYEGYTPTYSPHMDRSAVCS
                     SCHTLITNTADLNGNATGGQFVEQATYHEYLNSNYPANNIKCQTCHMPQLPDPVVIAN
                     GYIALTPRYPFNQHTFAGANHFMLQLIRDNKAALDVQVEDARFDSTLAATAQLLQERS
                     VALSVNFDSTASDTSYYQVRLENKTGHKFPSGYPSRRAVLQFIVLDNAGDTVFASGRY
                     DSDARVTDEDAGFESHHNVITREDQAQIYEMVMGDVNYDFTSVLERAAHLLKDNRIPP
                     AGFTTSHYAYDTVAISADALADPDFNKDNATEGTGVDIVHFHVPLNGAVGPFRVITSL
                     YYQSVPP"
BASE COUNT          375 a          540 c          422 g          398 t
ORIGIN      
        1 gatttcgatg aatttttgat taagtatttg gcgcttttat cccgcatatc ctgtttttcg
       61 gcggttttct gccaagacat tttgcatgca cggcctttca gacagacctg ctccaacagc
      121 cggaaaccat tgggttttca cggttttttt gattaaaatt gtcggacttc ccagccagtc
      181 agcctgcagc aatgcgccat tacctgatcg ccctgttttg cctcggggca ttcattttat
      241 ttgtcagctc cattccggac gacgagccgc gtccgcgttt ccattcaccg gctgaactcg
      301 aagccttaca acaacgcgtt cactcaccac tggttccggg tgaatggtac atgacgtctg
      361 caagttgccg cggttgtcac ggacacgaca cccttggtct ggcgaacatc aacgagaacg
      421 gcgaagacgt gaacctcttt tcgcattggg aaagttccat gatggcactc agcgcgaagg
      481 atccgctctg gcgggcgaag gtcagccagg aaattctcgt caaccccgga catgccgcgg
      541 agttgcagga caaatgcacg tcctgccatg cacccatggg acggtacaat tcacgttttc
      601 gtggtaacgc ccactacggt ctgaccgatc tatatgccga cagtctcgga ctcgatggag
      661 tctcctgtgc aggatgtcat accatcgatt cgacagtcgg gctaaccttc tccggtgaaa
      721 ttccatacga caccacccgc acgatctacg gccccttcac tttcccgatg accgggccaa
      781 tgcagttgta cgaaggctat acgccgactt acagtccgca catggaccga tcggccgttt
      841 gctcctcctg ccatacactc attaccaaca ccgccgacct caatggaaac gctaccggcg
      901 gtcagtttgt cgagcaggct acttatcacg agtacctgaa ctccaattat ccggcgaaca
      961 acatcaagtg ccagacctgc cacatgccgc aactcccgga cccggtggtc atcgccaacg
     1021 gctacatcgc ccttacgccg cgttatccgt tcaaccagca cacttttgcc ggtgccaatc
     1081 acttcatgtt gcaactcatt cgcgacaaca aagcggcact ggatgtccag gtcgaggatg
     1141 cgcgtttcga cagtaccctg gccgccaccg cgcaactcct gcaggaacgt tccgtggcgc
     1201 tttccgtcaa tttcgacagc acggcctccg acaccagcta ctatcaggtt cgattggaaa
     1261 acaagaccgg ccacaaattc ccttcgggct atccatcacg ccgggcggtg ttgcagttca
     1321 tcgtcctgga caatgcgggt gataccgttt ttgcctcagg tcgctacgac agcgatgcgc
     1381 gcgtaaccga cgaggatgcg ggcttcgaaa gtcaccacaa tgtgattaca cgggaggacc
     1441 aggcccagat ctacgaaatg gtgatgggtg acgtgaacta cgatttcact tccgtactcg
     1501 agcgtgccgc tcatctgctc aaggacaacc ggatcccgcc cgccggattc acgaccagcc
     1561 actatgccta cgacaccgtt gccatttctg cggatgccct ggccgatccc gacttcaaca
     1621 aggacaacgc tacggaaggt accggtgtcg atatcgttca cttccacgtt ccgttgaacg
     1681 gcgccgtggg ccctttccgg gtcatcactt ccctctatta ccagagtgtg ccgcc
//
LOCUS       DAOTON010000663         1733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_53794_length_1733_cov_3.668653, whole genome shotgun sequence.
ACCESSION   DAOTON010000663 DAOTON010000000
VERSION     DAOTON010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53794_length_1733_cov_3.668653"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..>1733
                     /locus_tag="P5292_11490"
     CDS             209..>1733
                     /locus_tag="P5292_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS39794.1"
                     /translation="MKFTNYSLQRSGPWLTGLLLMFFLSMGSIARAQVPCYTGFAYTT
                     SGSPYQLSFYDSSYDANGDRPVSWQWSFGDGTASTAQNPSHLYNSNGTYVVCLTATFA
                     TGCWTTTCDSIYVGQTLACNASYTFTVSPAGVYDFQNTSIGSGGLTYSWDFGDGTTST
                     VANPSHTFPAPGTYTVCLSIADGAGCSDTYCTAITINGTGSCQASFTTSTSGNTATFT
                     GNPSPAGAICTWDFGDGSFGTSIGNASLSHTYNANGAYTVCMTVTDSMQACTDTYCDS
                     LYVNGGGAITCSAAFQGAWVAGTMLVNFTDYSVSNDSIVSWAWDFGDGATSNQNNPSH
                     AYATPGNYQICLTITTASGCSSTFCDRVTVGGGGTGCTASFTANVSPSGAVVFTNTSS
                     PLNVLCTWDFGNGGTATSYGSNPVTWQYNANGVYVVCLTITDSMCTDTYCDSIVVGGA
                     LGCTANFQYIPDSTGTTVSFYDGSSGGATAWQWSFGDGTSSTSQNPVHTYAVTGTYTV
                     "
BASE COUNT          330 a          548 c          436 g          419 t
ORIGIN      
        1 tagtaactag taattagtaa ttagggactt ccaacaagtt tctagtttct agttaccagt
       61 tactaattac taaactccag cccgctaccc caatggcctt tcttggacgc gtaagatttt
      121 ccacacttcg ccttgtacct gcgttgttgc ccatctactt ttggatcatc gaatcaaacg
      181 catccaacca accctaaatt ctagtaccat gaagtttacc aattactcct tgcaacgcag
      241 cggtccctgg ctgaccggct tgttgctgat gttcttcctg agtatgggca gtattgcccg
      301 tgcacaggtg ccgtgttata ccggtttcgc ttatacgacc tccggttctc cttatcagct
      361 atcgttctac gattcttcct acgacgccaa cggtgaccgt ccggtttcct ggcagtggag
      421 cttcggcgac ggtactgctt ctaccgcgca gaatccttcg cacctgtaca actccaacgg
      481 cacgtatgtg gtctgcctga ctgccacgtt tgctactggt tgctggacga ccacctgcga
      541 ttccatttac gtaggacaga cgcttgcgtg caacgcttcg tacaccttca cggtcagccc
      601 ggcgggtgtg tacgatttcc agaacaccag catcggctcc ggcgggttga cgtattcctg
      661 ggacttcggg gatggcacca cctcgacagt ggccaacccg tcgcatacgt tcccggctcc
      721 gggtacgtat accgtttgcc tgagtattgc cgacggcgcg ggttgcagcg atacctactg
      781 cacagccatc accatcaacg gcaccggttc ctgccaggca tcgttcacca cttcgaccag
      841 cggcaacacc gcgaccttca ccggcaaccc ttctccggcg ggcgcgatct gtacctggga
      901 ttttggtgat ggttcctttg gcacctccat cggtaacgcg agcctctcgc atacctacaa
      961 tgccaacggc gcctatacgg tctgcatgac ggtgacggat tccatgcaag cctgcaccga
     1021 cacctattgc gattcccttt acgtgaacgg cggcggcgcc atcacctgtt ctgcggcctt
     1081 ccagggcgcg tgggtggcag gaacgatgct ggtgaatttc accgactact ccgtctccaa
     1141 cgattcaatc gtgagctggg cctgggattt cggcgacggc gctacgagca accagaacaa
     1201 cccttcacat gcctatgcta cgccgggcaa ctaccagatc tgcctgacca tcaccacggc
     1261 ttccggttgt tcctccacgt tctgtgaccg tgttaccgtc ggtggcggtg gtacgggttg
     1321 tacggccagc ttcacggcca atgtgagtcc ttccggcgcg gtggtcttca ccaacacttc
     1381 ttctccgctg aacgtgctct gcacctggga ttttggtaac ggcggtaccg ccacttcgta
     1441 cggcagcaat cctgttacct ggcagtacaa cgccaacggt gtgtatgtcg tctgtctgac
     1501 catcaccgac tccatgtgca ccgacacgta ctgcgacagc atcgtagtcg gcggcgcgct
     1561 cggttgcacg gcaaacttcc agtacattcc ggacagcacc ggcacgaccg tcagcttcta
     1621 cgacggctct tccggcggtg ccaccgcatg gcagtggtcg ttcggcgacg gaacttcgtc
     1681 gaccagccag aacccggttc atacctatgc ggtcaccggt acctacacgg ttt
//
LOCUS       DAOTON010000664         1726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_54101_length_1726_cov_2.507481, whole genome shotgun sequence.
ACCESSION   DAOTON010000664 DAOTON010000000
VERSION     DAOTON010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54101_length_1726_cov_2.507481"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="P5292_11495"
     CDS             <1..347
                     /locus_tag="P5292_11495"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HRS39795.1"
                     /translation="SAHSFDGSIRSAVIRNNHEYIRSIQAGQLPLERESIAPASVFNE
                     YLLTRLRTQWGVPFEDVRARFGSAALKQVMEQLEKVRETGWLESYAGGFRLSRQGKFF
                     ADTVAAGFFKDV"
     gene            complement(334..>1726)
                     /locus_tag="P5292_11500"
     CDS             complement(334..>1726)
                     /locus_tag="P5292_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRS39796.1"
                     /translation="DSLDVIVTNPAGYRSASNGILVLDTIIGNERVTHWQHRYPIATY
                     LVAIAVTNYARFNLTVPLPGGDVPVINLCFPEDSVYAATGASVVIGQMQLFDSLFGAY
                     PFRQEKYGQAQFGWGGGMEHQTMTFLGSWEKELIAHELAHQWFGDMITCGSWEDIWLN
                     EGFATYLSGLCYEFLEPELWKPFREKLVANGTQLDEGSVFCDDTTSVSRIFSGALSYS
                     KGAMVLHMLRQLIGDSAFFTGVRHYALDPTLQYSFSRTTTLIAHFESACQCDLDLFFN
                     EWYAGSGFPRYTAYWENLEQNKVRVQLFQSGSAGDGLFFHNPIELGFSGAAQDTVVRI
                     LPQSSGAFYEFEPGFRVSDITIDPHVNTFTAGNRVYRKAVNAEDELVLFPNPTDREIT
                     IRCDRPSLFNRTVSVNLFSTDGRLVYSEKTAFTPTASCSIYLDDLPSGLYLVELTDDQ
                     GLRWTRKLGVTHP"
BASE COUNT          409 a          448 c          483 g          386 t
ORIGIN      
        1 catccgctca ttccttcgac ggcagcatcc gttcggccgt cattcgtaac aatcatgaat
       61 atatccgcag cattcaagcc ggccaactgc cgcttgaacg ggagtctatc gctccggcct
      121 cagtattcaa cgagtatctc ttgacccgtc tgcgtacgca atggggagtg ccctttgaag
      181 atgttcgggc gcgattcgga tcagccgcat taaagcaggt aatggaacaa ctggagaaag
      241 tgcgggaaac aggttggctg gagtcctatg ccggaggttt ccgtttgtcc cggcaaggaa
      301 aattcttcgc cgatacggtc gcagcgggat ttttcaagga tgtgtaacac ccaacttccg
      361 ggtccatcgc agaccctggt catcggtcaa ctccacgagg tacaagccgg aaggaagatc
      421 atctaagtat atcgaacagg atgcagtggg ggtgaacgct gttttttcgg aatacaccaa
      481 tcgcccatcc gtactaaaca gattgaccga tacagttcgg ttgaacaggc tcgggcggtc
      541 acagcgaata gtgatttccc ggtcggtggg attgggaaat agtacgagtt catcttcagc
      601 gtttacagct ttccgataca ccctgttccc ggcagtaaag gtattcacgt gtggatcaat
      661 cgtgatatcg cttacgcgga atcccggttc gaattcatag aacgcaccag agctttgtgg
      721 tagtatgcgt acaacggtat cctgagctgc tcctgaaaac cccagttcga tggggttgtg
      781 aaagaatagg ccatcaccgg cgcttccgga ttgaaacaac tgtacccgga ccttgttttg
      841 ttccaggttc tcccaatagg cggtgtaacg cgggaagccg ctacctgcgt accattcatt
      901 aaaaaacagg tcaaggtcgc attggcaggc ggattcgaag tgcgcgatca aggtagtcgt
      961 acggctaaag gaatattgaa gggtcggatc cagcgcatag tgacggacac cggtgaagaa
     1021 agccgaatct ccgatcagtt gccgcagcat gtggagtacc atcgcgcctt tggaatagct
     1081 cagtgcgcca ctgaagatcc tgctgacgga agtggtgtca tcgcagaata cactgccttc
     1141 gtcaagctgc gtgccgtttg ccaccagttt ttcccggaac ggtttccaca gctcgggttc
     1201 gaggaattca taacaaagtc cgctgaggta ggtggcaaag ccttcattga gccagatatc
     1261 ttcccaactc ccgcaggtga tcatgtcgcc gaaccactga tgggcaagtt cgtgggcgat
     1321 cagctctttc tcccaactgc caaggaatgt catggtttga tgttccattc cgccacccca
     1381 gccgaactgc gcctgtccgt atttttcctg ccgaaaggga taggcgccga acaaggaatc
     1441 gaacagttgc atctgaccga tcactacgga agcgcccgtg gcggcataga cgctgtcctc
     1501 gggaaagcaa aggttaatca cgggaacgtc gccacccggc agcggaacag tcaggttgaa
     1561 gcgcgcatag ttggtaacgg cgatcgccac caggtacgtg gcaataggat agcggtgctg
     1621 ccaatgcgtg acgcgttcgt taccgatgat cgtgtcgagg accaggatgc cgttcgacgc
     1681 gctgcgataa ccggccgggt tggtgacgat gacatcgagc gagtcg
//
LOCUS       DAOTON010000665         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_54208_length_1723_cov_3.297962, whole genome shotgun sequence.
ACCESSION   DAOTON010000665 DAOTON010000000
VERSION     DAOTON010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54208_length_1723_cov_3.297962"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="P5292_11505"
     CDS             complement(<1..292)
                     /locus_tag="P5292_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39797.1"
                     /translation="MLLIGELIQLRAMEPADLNLLYRWENDTSIWSVSGTLAPFSRYV
                     LEEFVSQAHQDIYTNKQLRLMIDLRYYDEEDGVTEEVRSIGCVDLFDFDPKNR"
     gene            complement(404..1198)
                     /gene="dapF"
                     /locus_tag="P5292_11510"
     CDS             complement(404..1198)
                     /gene="dapF"
                     /locus_tag="P5292_11510"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRS39798.1"
                     /translation="MIPSIPFVKYHGTGNDFILLDEREIDYQMNTEEISKLCHRHFGI
                     GADGLISLRKAPDADFRMVYYNADGREGSMCGNGGRCIVAFARSLAVIGERTRFIGVD
                     GYHEAMFLPDKPGFVSLKMSDVAAPRKRVGYDFIDTGSPHVVVAVRDLAGYPVVANGR
                     SIRNLPEFSPGGTNVNFVEAVNDSLFIRTYERGVEDETMSCGTGVTAVVLTLAALAQL
                     PASAREAQVMTPGGSLSVAFDRSEMGFSNVWLTGPAQRVFEGTYTR"
     gene            complement(1195..>1723)
                     /locus_tag="P5292_11515"
     CDS             complement(1195..>1723)
                     /locus_tag="P5292_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39799.1"
                     /translation="PAPPVAKAPANKPSAEVVAAEEKTGDESVLPIDSGQTAEGKDTI
                     YDAAGEIVVRKDQLLISMDLHVSSDEPGENAPTLEDKALERLNPAAGLVKSESREPTI
                     ATEFWISPVNYRGYRLLDNKLILYGIESPDRVSLLRSNDRLLLKYGNELFILSESEAF
                     QPYPRAQEAARSENR"
BASE COUNT          375 a          505 c          468 g          375 t
ORIGIN      
        1 gccggttctt gggatcgaaa tcgaacagat ccacacagcc gatgctgcgg acctcttccg
       61 ttaccccgtc ctcttcgtca taataccgaa ggtcgatcat caggcgcaac tgcttgttcg
      121 tgtagatatc ctggtgcgcc tggctgacga actcctccag cacatagcgt gaaaaggggg
      181 ccaacgtgcc gctgacactc cagatggacg tatcattctc ccaacggtag agaagattaa
      241 ggtcggcagg ctccatggcc cgcaattgaa tcaattcacc gatcagcaac ataccggatt
      301 ccttcgatgc ccaattgggc ttgcatttga cacgacgaaa ataaggaagg gaacgccagc
      361 agcgcttcgc tcgcgctctt tttttaaccg gttgttgtga accttatcgg gtataggttc
      421 cctcgaaaac ccgctgcgcc ggacctgtca gccatacgtt tgaaaaaccc atctccgaac
      481 ggtcgaacgc gacggataag gatccgccgg gtgtcatcac ctgcgcttcg cgcgcggagg
      541 ccggcagctg agccagcgcg gcaagggtca gcactacggc agtcactccg gttccacacg
      601 acatggtttc gtcttccact ccccgctcgt acgtgcgaat gaacaagctg tcattgaccg
      661 cttccacgaa attgacgttg gtacctccgg gcgagaactc cggcaggttc cggatcgaac
      721 gaccgttcgc cacgaccggg tagccggcaa gatcgcgtac agctaccaca acatgcgggg
      781 agccggtatc gatgaagtcg taaccgactc gcttccgggg cgctgctaca tccgacattt
      841 tcaagctcac gaatccgggc ttgtccggaa ggaacatcgc ttcgtgataa ccgtccactc
      901 cgatgaagcg ggtccgctcc ccgatgaccg ccaggctgcg ggcgaaggcg acaatgcatc
      961 gaccaccgtt tccgcacatg gaaccttccc ggccatcggc gttatagtag accatacgga
     1021 agtcagcatc cggtgcttta cgtaacgaga tcagtccgtc ggctccgatc ccgaaatgcc
     1081 gatgacacag cttgctgatc tcttccgtat tcatctgata atcgatttcc cgttcatcga
     1141 ggaggatgaa atcattaccg gtaccatggt acttcacgaa cggaatggat gggatcatcg
     1201 gttttcgctg cgtgcggctt cctgggcgcg aggatatggt tgaaacgctt cggattccga
     1261 caagatgaat agttcattcc cgtacttcag gagcaatcga tcattggagc gcagcaagct
     1321 gacccgatca ggggattcga tgccatagag gatcagctta ttatcaagaa gacgataacc
     1381 gcggtaattg accggtgata tccagaactc ggtagcaatg gttggttcac ggctttcgct
     1441 ttttacgaga cccgcagcag ggttgagccg ttccagcgcc ttatcctcga gcgttggcgc
     1501 gttctccccg ggctcatcac tggatacgtg gagatccatg gagatgagca actggtcttt
     1561 cctaacgacg atctcacccg cggcgtcgta aatggtatcc ttgccctcgg cggtctgccc
     1621 ggaatcgatg ggcaggaccg actcatcgcc agtcttctcc tccgccgcga ccacctccgc
     1681 cgaaggtttg ttcgcgggtg cctttgcgac aggcggggca ggg
//
LOCUS       DAOTON010000666         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_54218_length_1723_cov_2.616307, whole genome shotgun sequence.
ACCESSION   DAOTON010000666 DAOTON010000000
VERSION     DAOTON010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54218_length_1723_cov_2.616307"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1647)
                     /locus_tag="P5292_11520"
     CDS             complement(<1..1647)
                     /locus_tag="P5292_11520"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRS39800.1"
                     /translation="MPNRLLHESSPYLLQHAHNPVDWYPWGEEAWEKARKENKLVLVS
                     IGYSSCHWCHVMERQSFEQEAVGKLMNEHFVCIKVDREERPDVDQVYMSAVQLMTGGG
                     GWPLNCFTLPDSRPIYGGTYFPRPQWEDLLVRLNHFYLTDPERARTYADELMNGLRQM
                     EQFAAKAGHPEFSMTTIDEMMVNWKRSFDPREGGPDRAPKFPLPNNYSFLLRYLSCAH
                     DPDTDAHVHLTLGKMAMGGIYDQIGGGFSRYSVDAEWKVPHFEKMLYDNAQLVTLYAE
                     AHRRSPNPLYETVIRESLAFIERELSDPNGGGYAALDADSEGEEGRFYTWEPEELRSV
                     IGRLRFDLPPGIDPVTLLFDYYRVGAEGHWEFDRNILLRKEHDDSFAARHQLSQETLL
                     AFLRTAKAGLLHSREKRERPGLDKKILLSWNALLLEAWCEAYRALGDPAFLENAIRLG
                     ERLRSHALADGRLPHLLENGQKPANPGFLDDYAFFAQGMISLYGVTFDEAWLWSGRNA
                     VAYALEHFSDPESPLLWYTSDLDPPLVTRKKEVHDNVIPAS"
BASE COUNT          361 a          471 c          520 g          371 t
ORIGIN      
        1 ggaagcgggg atcacgttat cgtgcacctc tttcttgcgc gtgaccaatg gcggatcgag
       61 atcggaagta taccaaagca acggactttc cggatccgaa aaatgttcca acgcatacgc
      121 aaccgcgttc cgtcccgacc agagccacgc ttcgtcgaag gtgacgccgt acaaggagat
      181 catgccctgc gcaaagaagg catagtcgtc gaggaacccc gggttggcgg gcttctgccc
      241 gttctccagc agatggggca aacgaccatc cgccagtgca tgtgaacgta atcgctctcc
      301 caaccgaatg gcattttcaa gaaacgccgg gtcgccgagc gcccgatagg cttcgcacca
      361 ggcttccagg aggagggcat tccaggaaag caggatcttt ttatcgagtc cgggtcgttc
      421 gcgtttttcc cgggagtgta gcagtcctgc tttcgctgtt cgtagaaacg cgagcaatgt
      481 ctcctgtgaa agttgatgcc gtgcagcgaa ggagtcgtcg tgttctttcc gtagcaggat
      541 gttgcgatcg aattcccagt gaccttcggc acctacgcga taatagtcga agaggagtgt
      601 caccgggtcg atacccggag gcaggtcgaa tcgcagtcga ccgattaccg atcgaagttc
      661 ctccggctcc caggtgtaga atcgtccttc ctcgccttcc gaatcggcat cgagggctgc
      721 gtaaccacct ccgttcgggt cactgagttc acgttcgata aacgccagtg attcgcgtat
      781 caccgtttcg tagagcgggt tcggcgaacg ccgatgagct tccgcataga gggtgaccaa
      841 ctgggcgttg tcatagagca ttttttcaaa gtgcgggact ttccattccg catctacgga
      901 atagcgggag aagccgccgc cgatctgatc gtagatcccg cccatggcca tcttcccgag
      961 ggtcaggtgt acatgcgcat cggtgtccgg atcatgggcg cagctgaggt aacgaagcag
     1021 gaaactgtag ttgttgggta agggaaattt cggtgcgcgg tccggcccgc cttcgcgtgg
     1081 atcgaagcta cgcttccagt tcaccatcat ttcatcgata gtggtcatgg agaattccgg
     1141 atgaccggcc tttgctgcaa actgctccat ctgccggaga ccgttcatga gctcatccgc
     1201 gtacgtgcgg gcgcgttcgg gatcggtgag gtagaaatga ttcaagcgga ccagcaaatc
     1261 ttcccattga ggtcgcggga agtacgtgcc gccgtaaatc ggacggctgt cgggcagggt
     1321 aaagcagttg agtggccagc cgccgccgcc ggtcatgagt tgcaccgccg acatgtacac
     1381 ctggtccaca tccggccgtt cttcccgatc gaccttgatg cagacgaaat gttcgttcat
     1441 cagcttgccc acggcttcct gctcgaagct ttgtcgttcc atgacatggc accagtgaca
     1501 agacgaatag ccgatcgaga ccagaaccaa cttgttctcc tttcgggctt tctcccaggc
     1561 ttcttccccc caggggtacc agtcgaccgg gttgtgggca tgctggagca ggtaagggct
     1621 cgattcgtgg agaagacggt tgggcatggg aaggagaggg acgagggacg agcgaggtgc
     1681 gaggcttgcc tgccgaagct ttagcgtagg cagggacgag gga
//
LOCUS       DAOTON010000667         1721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_54320_length_1721_cov_2.291717, whole genome shotgun sequence.
ACCESSION   DAOTON010000667 DAOTON010000000
VERSION     DAOTON010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54320_length_1721_cov_2.291717"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="P5292_11525"
     CDS             complement(<1..412)
                     /locus_tag="P5292_11525"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRS39801.1"
                     /translation="MTNKEIARAFKLAGQLLELHDENPFKVRSFQNAAFKVERLPEAL
                     ETMDAAAIAGIEGIGKSLQARILELKERGSFDELDELKSKTPEGILEILSIKGLGPKK
                     VRTLWQELEIQSVGELLYACQENRLVTLKGFGEKT"
     gene            complement(421..>1721)
                     /gene="sppA"
                     /locus_tag="P5292_11530"
     CDS             complement(421..>1721)
                     /gene="sppA"
                     /locus_tag="P5292_11530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRS39802.1"
                     /translation="PEGGVELRGLHTELMFFKGALDKLGIEPEVIRHGKFKSAIEPFT
                     LDKMSPENREQISRLLGSVWNTWLGNISADRKLPKEELQAIADQYRSRKAKDALDLRL
                     IDKTAYFDEVTDDLRKQCGLEEKEKVRYVELQKYNKAYVKPEKEFSNRKIAVVYAVGE
                     IRSGQGSDEVIGSERISEAIRKARLDTTVKAIVLRVNSPGGSALASEVIWREALLARK
                     AKPLVVSMGDLAASGGYYISCIADTIVAQANTLTGSIGVFGLLFNTQNMWKDKLGITF
                     DTVRTGRFADIGSLSRPLTAEERAIYQEEVEQIYNVFIGHVAEGRNMTTAAVDSIGQG
                     RIWSGTDAKQIGLVDVIGGLETAKSIAAKMAGLDNYRTVNYPEQKEFLQKLMEDFSAD
                     AKAYFAKEELGESYRYYVKLREVLNSQGIQARLPYELFIY"
BASE COUNT          289 a          474 c          508 g          450 t
ORIGIN      
        1 gcgttttctc tccaaagcct ttcagggtaa caagccggtt ctcctggcaa gcgtaaagca
       61 gttcgccgac agattggatc tcgagttcct gccagagtgt gcggaccttc tttggaccca
      121 gccctttgat ggaaaggatc tcgaggatgc cttccggtgt tttgcttttc agttcgtcga
      181 gttcgtcgaa ggaaccgcgt tcttttagtt ccaggatgcg cgcttgcagg ctcttcccga
      241 tcccttcgat cccggcgatg gcggctgcgt ccatggtttc gagcgcttcc ggcagtcgtt
      301 ccactttgaa ggccgcgttt tgaaaagagc ggaccttgaa cgggttttcg tcgtgcagct
      361 cgagcagttg tccggccagt ttgaaggcgc gcgcgatctc cttgttcgtc atgcgccggt
      421 tcagtagatg aagagttcgt acggcaggcg tgcctgaatg ccctggctgt tgaggacttc
      481 gcgcaatttc acgtagtaac ggtagctttc gcccagttct tccttcgcga agtaggcttt
      541 cgcgtcggcg gagaagtctt ccatcaactt ctgcaggaat tccttttgct cgggatagtt
      601 gactgttcgg tagttgtcga gtccggccat cttcgccgcg atggatttcg cagtttccag
      661 tccgccgatc acatcgacca gcccgatctg tttcgcgtcc gtacctgacc agatgcggcc
      721 ctggccgatg ctgtcgaccg cggcggtggt catgttccgg ccttcggcca cgtgaccgat
      781 gaacacgttg tagatctgtt ccacttcttc ctgataaatg gcgcgttcct cggcggtcag
      841 cggacgagag aggctgccga tatcggcaaa gcggcctgtg cgtacggtgt caaaggtgat
      901 gccgagtttg tccttccaca tgttctgggt gttgaacagc agtccgaaca cgccgatgga
      961 gcctgtaagc gtgttggctt gcgcgacgat ggtgtcggcg atacaggaga tatagtagcc
     1021 tccggaagcc gccaggtcac ccatggatac caccagcggt ttggctttgc gtgccagcaa
     1081 ggcttcgcgc cagatcactt cggaggcaag ggcgctgcca cccggtgaat tcacgcgcag
     1141 gacgatggct ttgacggtcg tgtcgaggcg ggctttgcga atggcctcgc tgatgcgctc
     1201 cgaaccgatc acttcgtcgc tgccttgtcc ggagcggatc tcgccgaccg catagacgac
     1261 ggcgatcttg cggttggaaa attccttttc gggttttacg tacgccttgt tgtatttctg
     1321 caattcgacg tagcgtactt tctctttctc ttcaaggccg cattgcttgc ggaggtcatc
     1381 ggttacttcg tcgaagtaag cggtcttgtc gatcaggcgg aggtcaagcg cgtccttcgc
     1441 cttgcggcta cggtactgat cggcgatggc ctgaagttct tccttcggga gtttacgatc
     1501 agcggaaata ttcccgagcc aggtgttcca gacgctgcca agcaggcggc tgatctgttc
     1561 cctgttttcg gggctcatct tgtcgagtgt gaacggttca atcgcgctct tgaatttacc
     1621 gtgccggatc acttccggtt cgatgccgag cttgtcgagt gctcctttaa agaacatcag
     1681 ttccgtgtgg aggccccgca gttcgacgcc gccttcggga t
//
LOCUS       DAOTON010000668         1720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_54362_length_1720_cov_2.711712, whole genome shotgun sequence.
ACCESSION   DAOTON010000668 DAOTON010000000
VERSION     DAOTON010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54362_length_1720_cov_2.711712"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="P5292_11535"
     CDS             complement(<1..495)
                     /locus_tag="P5292_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39803.1"
                     /translation="MRQRLQLRPRVALRFHTALVFTFASVVLASWAFFGFNLAGNGDA
                     TASDRSAIPVIKKEVAGSLQESDPSIATLTAGLDNPTPVASRRYHQMRQSEYDVRSES
                     FRAIVGSPAQGKKQLSVLRASANPAGRTINVPVTVDNDGKAVLRIFSSSGYCMSSREV
                     NLAAG"
     gene            complement(607..>1720)
                     /locus_tag="P5292_11540"
     CDS             complement(607..>1720)
                     /locus_tag="P5292_11540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39804.1"
                     /translation="MGNMHGASSAGSEKWYVPLSTGQWNTATRRGVVTSNGASPREGV
                     LLVYGPAYGNSSNGWVMYEAGHDLSTGGSSATDRVAAQRAYFNFILLAGTAKKININA
                     TVTATLPSGASGTASATVSSGTPPYSYQWTSQLGGTFANSSAATTAYTAPTVGGNTTD
                     VVTLRVTDACGRVSLYTQFINITFSPLPVSLVSFEAKRNGQQVLTSWVTASEVNNDFF
                     TIERSTDGSVFQALNRVAGRGTTSETSTYRWTDPQPPAGICYYRLRQTDYDGRSETFP
                     SVMVEATRSGSRDIAIYPNPVRDRFMLPVTVESDCQATLRIYNATGACVQQRLLNLQR
                     GSNTVNGTTADLPAGNYVLMLESEGLLTKSRFSLIR"
BASE COUNT          383 a          491 c          514 g          332 t
ORIGIN      
        1 ccccgcagcc aggttcactt cgcgcgaact catgcaatag ccggaggagg agaagatgcg
       61 cagtaccgct ttgccgtcgt tatcgaccgt gaccggcacg ttgatcgtac gaccggcggg
      121 attcgcggag gcccgcagca cggacaattg ctttttaccc tgagcaggcg aaccgacaat
      181 ggctcggaac gattccgagc ggacatcgta ctcgctctga cgcatctgat ggtaacgccg
      241 actggctacc ggtgtgggat tatccaatcc ggccgtgagg gttgcgatgg aaggatccga
      301 ctcctgcaga ctaccggcga cctctttttt gatcaccgga atggcggacc gatcggatgc
      361 cgttgcatcg ccgttgccgg ccaggttgaa gccgaagaag gcccaactgg ccaatacgac
      421 cgaagcgaag gtgaaaacga gcgctgtatg aaaacgcagc gcgacgcggg gacgaagttg
      481 aagacgttga cgcatgggaa gtgtttaaaa ggcggggagc gccgccgtaa cgctcaaatt
      541 aaccctgtgc aggccgaagg cgcggcactt caccgccaaa ccttacactg aaagaaaaga
      601 cccgaattat cgaatcagcg aaaagcgcga tttggtcagc aagccttcgg attccagcat
      661 caacacataa ttgccggccg ggaggtcggc agtcgtgcca ttcacggtat tggatccgcg
      721 ctgtaagttg agcagccgtt gttgcacgca ggctcccgtt gcattgtaga tacgcagtgt
      781 tgcctgacaa tcggactcaa ccgttaccgg caacatgaac cggtcgcgca ccggattcgg
      841 gtagatggca atatcccggc tgcccgacct tgtcgcttcg accatgaccg aagggaaagt
      901 ctccgatcgt ccgtcgtaat cggtctgccg taaacgatag taacaaatcc ccgcaggcgg
      961 ctgtggatca gtccacctgt acgtactagt ctcgctggtc gtgccccggc ccgctacacg
     1021 attcagggcc tggaacaccg aaccgtcggt cgagcgctcg atggtgaaaa aatcgttgtt
     1081 cacttcagaa gccgttaccc agctcgtaag cacctgctgc ccgttccgtt tcgcttcgaa
     1141 cgatacgagc gacaccggca gcggactgaa ggtgatgttg atgaattgcg tatacaggga
     1201 gacgcggccg caggcgtcgg tgacacgcag cgtaacaaca tcggtcgtgt tcccgccaac
     1261 cgtcggcgcc gtgtaagcgg tggtggcggc ggagctgttg gcaaacgtgc ctccgagctg
     1321 actggtccat tgatatgaat acggcggcgt accggatgaa acggtagccg aagcggttcc
     1381 tgaggcaccg gaaggaagtg tagcggtaac cgtggcgttg atgttgatct tcttcgccgt
     1441 cccggcgagg aggatgaaat tgaaatacgc gcgttgcgcg gcaacacgat ccgtagcact
     1501 ggatccaccg gtggagagat cgtggcctgc ctcgtacatc acccagccgt tactgctgtt
     1561 gccataagcc ggtccgtaca ccaacaggac cccttcccgt ggactcgcac cattggaggt
     1621 aacgacaccc cgtcgggtag ccgtgttcca ttgaccggtg gaaagcggca cataccactt
     1681 ttccgaaccg gcgctggagg caccgtgcat attacccatg
//
LOCUS       DAOTON010000669         1720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_54365_length_1720_cov_2.562162, whole genome shotgun sequence.
ACCESSION   DAOTON010000669 DAOTON010000000
VERSION     DAOTON010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54365_length_1720_cov_2.562162"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="P5292_11545"
     CDS             <1..423
                     /locus_tag="P5292_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39805.1"
                     /translation="LIAYTMNATMDSVLNKQYFLRYEYGRVRDACAGPDGSLYFIAYD
                     RSSPRIMRVRNALNTGVSNSETKETVLFPNPASEYLQYQTALPATMTTCIIRDLQGRF
                     VLHRNLQGDQGILNVGHLKSGTYSFQIGSSHPVLLVIL"
     gene            951..>1720
                     /locus_tag="P5292_11550"
     CDS             951..>1720
                     /locus_tag="P5292_11550"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRS39806.1"
                     /translation="MLADPNGTLTIGGTPGGTAWLINGNSGLNGGNTTTAGTNFIGTL
                     DAQNIDFRTNNLVRGRFSSLGEFFVGTLNTVIAGDLMNAVGNTTFPWAVNGYSPAGVN
                     GGGVYGQIQTGNTTTYAAVQGEYQGTSASGAGVRGIAITTTAIGVAGTEPTLAGWAVL
                     GNGDFGAILPGNYYTISDQRLKTNISPLTGSLDKVMRLKCYSYDANKEAYPKYFASSR
                     KMMGFLAQELEQVIPEAVADKTIPSLNTNRTAPDADVET"
BASE COUNT          446 a          431 c          416 g          427 t
ORIGIN      
        1 ctcatcgcct acacgatgaa cgcaacgatg gattccgtcc tcaacaagca gtactttctg
       61 cgttacgagt acggtcgcgt ccgcgatgcc tgcgccggcc ccgacggaag tttgtatttc
      121 atcgcctacg accgttcctc gccgcggatt atgcgggtgc ggaacgcgtt gaatacgggt
      181 gtttcgaatt cggagacaaa agaaaccgtg ctatttccaa atcctgcaag tgaatatctt
      241 caatatcaaa ccgcgcttcc ggcgacgatg actacctgca tcatacgtga tctccaggga
      301 aggtttgtat tgcaccggaa cctgcaaggc gaccagggaa tactgaacgt cggtcatttg
      361 aaatcaggaa cctattcttt tcagatcgga agcagccatc ctgttttgct ggttatcctt
      421 tgatgagtcg atagggtgtt gaagttaact tgaatcatcg gcaaaagctc aagcatcggg
      481 aatgcatatt ggtagggccg ggggatattc atgcttctta actaacgatc cagtacgaca
      541 ctgttctttt attcgatttt gggcggtagt acttatcaag aagcgaatga accttaccac
      601 ttcagggact ctcaacttgg aggttcaatc ttaacttgaa ctaagctacc cagcacatat
      661 taattcgatt taatgcggca ccatttcacc ccctgtcaaa atcattttac caaaaaatcc
      721 atagaatccc ctcaattatt accagaaatt gttatctttt cggattaaaa ccaagaaaga
      781 tgaacttacc catccgtttg tcgaccctta ttcttgcctg cacactaggg gcacttccgt
      841 ttcatggcta tgcacagaat gttggaattg gaacttcatc cccgctagac aaacttcatg
      901 ttgttggcac gattcgatcg acagctcttt cagggattgg taatcggctt gtgcttgccg
      961 atccgaatgg aacattaacg ataggcggca caccgggtgg tacggcctgg ttgatcaacg
     1021 gcaattccgg actaaacggc ggcaatacca caaccgccgg taccaacttc atcggaacgc
     1081 tggatgcgca gaatatcgat ttccgcacca acaacctggt acgtggcagg ttcagttcct
     1141 tgggtgaatt ttttgtagga acactcaata cggtaattgc cggagatctg atgaatgcgg
     1201 ttggtaacac aacctttcca tgggcagtga acggctatag ccccgcaggt gtcaacggtg
     1261 gcggggttta cggtcaaatt caaactggca atacaaccac ctacgctgcc gtgcagggtg
     1321 aatatcaggg caccagtgct tctggagccg gtgtacgcgg tattgccata acgacaacag
     1381 cgatcggtgt agccggaacc gagcctacgc ttgcaggctg ggccgtcttg gggaatgggg
     1441 attttggcgc aattcttccc ggcaactatt atacgatctc ggatcagcga ttaaagacca
     1501 acatttcacc attgaccggt tcgctcgaca aggtaatgcg cctgaaatgt tactcctatg
     1561 acgccaacaa ggaagcatat ccaaagtact tcgcaagttc ccgaaagatg atgggcttcc
     1621 tggcgcaaga actcgaacag gtaattccgg aagcggtggc tgacaaaacg attccgtccc
     1681 tgaacacgaa tcgcaccgcg cctgatgcgg atgtagaaac
//
LOCUS       DAOTON010000670         1717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_54486_length_1717_cov_3.114922, whole genome shotgun sequence.
ACCESSION   DAOTON010000670 DAOTON010000000
VERSION     DAOTON010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54486_length_1717_cov_3.114922"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="P5292_11555"
     CDS             <1..729
                     /locus_tag="P5292_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRS39807.1"
                     /translation="QVKTGGWVIHRKGLPLPSDGRVVPYAIREASLISGKDIRIDSHR
                     YTFTYSGLGAEWKNLWTRLPGYHNVENAVAATSVARILDLPEEKVRKGLESYTGVLRR
                     FDYQVDTDRHVYIDDYAHHPEELKACILSARELYPGKKITGIFQPHLYSRTRDFVDGF
                     AESLSLLDELLLLEIYPARELPIEGVNAKMILDRMTIPARRIVSKQEALDLVKKEQPE
                     VLLTLGAGDIDQLVAPLRDMLNKL"
     gene            733..1551
                     /locus_tag="P5292_11560"
     CDS             733..1551
                     /locus_tag="P5292_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39808.1"
                     /translation="MWKRFQSIDWKRAGRISGWSALGIVFVVSVGFTGSRQDSMACTG
                     VHVHITDTIGHSFVEVDDVEAMIRDKYGAVEGKSLHAINTALLESIIRNNPFVKTAEV
                     FSSIDGKLYVEVIQRTPIVRVINQLGESFYIDEQGTFMPLSGQYTARVPVANGYLFDR
                     EAERNILQYSEEQRADTSIKRSRIDEVFRVAQFLQGDPFWLSQTEQLYVNLNGDIELV
                     PRVGNHTILLGDASRLEVKFRNLRWFYREALNKKGWGEYKTINLKYHDQVVAAH"
BASE COUNT          448 a          417 c          472 g          380 t
ORIGIN      
        1 caggtcaaga ccggtggctg ggtcattcac cgaaaaggct tgcccttgcc gtccgacggc
       61 agggtggtgc cgtatgcgat ccgggaagct tcgctgatct ccggaaagga tattcgtatc
      121 gattcgcatc gatacacctt cacctattcg ggcttagggg ccgaatggaa gaacctgtgg
      181 acccgattgc ccggttatca caatgtcgaa aacgcagttg cagctacatc cgtagcgcgg
      241 atactggact tgccggagga gaaagttcga aaaggacttg agtcctacac cggtgtgctc
      301 cgccggttcg actaccaggt cgacacggac cgacacgtct atatcgacga ttatgcccat
      361 catccggaag aattgaaggc ttgcattctg tcggccagag aattgtatcc cgggaaaaag
      421 atcacgggca tctttcaacc ccacctgtac agcaggaccc gggatttcgt cgatggcttt
      481 gcagaaagtc tctcgttgct ggatgaatta ctgctattgg agatctatcc ggcacgcgaa
      541 cttcctattg agggggtgaa tgctaaaatg atcctggacc gcatgaccat tccggcccga
      601 cggatcgtca gcaagcagga agcacttgat ttggttaaaa aagaacaacc cgaagtactg
      661 ttaacgcttg gcgccggtga tattgatcaa ctggtagcgc cattgcgtga tatgctcaac
      721 aagctataac ctatgtggaa gcgatttcaa tccattgact ggaaacgtgc aggccgcatc
      781 agcggctggt ccgccctcgg aatcgtattc gtcgtatcag tcggattcac cggctcacga
      841 caggattcga tggcctgtac cggtgttcat gtacacatta cggatacgat cggtcacagt
      901 ttcgtggaag tcgatgatgt agaagcgatg atccgggaca agtacggagc ggttgaggga
      961 aaatccttgc atgcaattaa cacggctttg ttggaaagta tcattcgcaa caaccccttc
     1021 gtgaaaacag ccgaggtatt ttcatccatt gatgggaagc tgtatgtcga ggtgattcaa
     1081 cgtacaccga tcgtgcgcgt gatcaatcaa ttaggcgaaa gcttctacat cgacgaacaa
     1141 gggactttca tgccactctc gggtcaatat accgcccggg tgcctgttgc aaacggttac
     1201 ttgttcgatc gggaagcgga aagaaacatc cttcaatatt cggaagagca gcgggccgac
     1261 acgtcgatca agcggtcaag gatcgatgaa gtcttccggg tggcacagtt ccttcaggga
     1321 gacccgttct ggctttcaca gacggaacaa ctctatgtga atctgaacgg tgacatcgag
     1381 ctggtcccac gggtgggcaa ccacacgatc ctgctgggag acgccagccg gttggaggtg
     1441 aagtttcgaa atcttcgctg gttctaccgc gaagcgttga ataaaaaggg gtggggggaa
     1501 tacaagacca tcaaccttaa ataccacgac caggtcgttg ccgcgcactg accgcaagcg
     1561 tgattaaagg ggacggtagc aaacaaaggg gacaccaacg gcattaaaat gatattggcc
     1621 agcacaatgg aaaacgagat cgtagtagga cttgacatcg gtacaaccaa aatttgtacg
     1681 attgtcggcc gtcgcaatga attcgggaag atcgaca
//
LOCUS       DAOTON010000671         1713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_54668_length_1713_cov_2.625452, whole genome shotgun sequence.
ACCESSION   DAOTON010000671 DAOTON010000000
VERSION     DAOTON010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54668_length_1713_cov_2.625452"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="P5292_11565"
     CDS             complement(<1..441)
                     /locus_tag="P5292_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016419878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRS39809.1"
                     /translation="MNRGPISQFMEKHYLHFNAAAMVDAAKGYEKHLLEGGKMMVTLA
                     GAMSTAELGKSLAEMIRQGKVDIIYGGALIDTVGQGGIIGEMALIGGEPRSATAVART
                     GCALTPIDERRFETLVQEAPYFAVMVMRVLVRRLRRSNELAAAER"
     gene            complement(488..1534)
                     /locus_tag="P5292_11570"
     CDS             complement(488..1534)
                     /locus_tag="P5292_11570"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39810.1"
                     /translation="MKINKLLISAILLSWTSIACAQSHQYKVSIIAFYNLENLYDTID
                     DPNKSDEEFLPGGNKHYTSEVYQDKLGKLSEVLSQVGTDKSPDGWAILGCAEIENDRV
                     LQDLISQPKLKERNLKIVHYDSPDLRGVDVGLLYNPKYFNPEHSEPLFVELHDPDGSI
                     RYTRDILYVKGNFNGEPMHIFVNHWPSRRGGEEASAPGRAKAAGIVRQKMDSITAIDP
                     KAKIVVMGDLNDDPVSPSVAKVIGSKGEKSEVQPGGMFNPWVKPYKEGIGTLAYNDSW
                     NLFDQIMISYGFLDQHQSGWFYREWHIFSKPWMMQTDGRWKGYPKRTYDFDRYAGGYS
                     DHFPTYLVLLKQID"
BASE COUNT          368 a          486 c          427 g          432 t
ORIGIN      
        1 ccgctcggca gccgcgagct cgttgctgcg ccgcagccgc cgcacaagca cgcgcatcac
       61 catcaccgcg aagtacggcg cctcctgcac aagcgtctcg aagcgccgct cgtcgatcgg
      121 cgtcagcgcg cagccggtgc gcgccacggc cgtggcgctg cgcggctccc cgccgatcag
      181 cgccatctcc ccgataatcc cgccctgtcc caccgtatct atcagcgcgc cgccgtagat
      241 gatgtccacc ttgccctgtc ggatcatttc agcaagtgat ttgcctagtt ctgccgtcga
      301 catggcgccg gcgagtgtaa ccatcatttt accgccttcg agcaggtgtt tttcataccc
      361 cttggcagca tccaccatgg cggcggcatt gaaatggaga tagtgtttct ccatgaactg
      421 ggagatcggt ccgcgattca tttttctatt ggtaattagg atagggaacg aatggaaagg
      481 acaggcatca gtcgatctgc ttgagcaaca ccaggtaggt cgggaagtga tcggaatacc
      541 cgcccgcata ccggtcgaag tcatacgttc gcttcgggta gcctttccaa cggccgtcgg
      601 tctgcatcat ccagggtttt gagaaaatat gccattcccg gtagaaccaa ccgctttgat
      661 gctggtcgag gaaaccgtac gaaatcatga tctgatcaaa cagattccaa ctgtcattat
      721 aagccagcgt gccgatgcct tccttatacg gtttgaccca gggattgaac attccgcccg
      781 gttgaacctc tgatttttcg ccctttgagc cgatcacttt ggctacactc gggctcaccg
      841 gatcgtcgtt gaggtcaccc atgacgacga tctttgcctt gggatcgatc gctgtgatgg
      901 aatccatctt ctgccttaca atgccagcag ccttcgccct tcccggagca ctggcctctt
      961 ccccgcctct gcgcgacggc cagtgattca caaaaatgtg catgggttcc ccattgaaat
     1021 ttccctttac gtaaaggatg tctcgcgtat aacggatgga accatccgga tcatgcagtt
     1081 cgacaaatag cggttcgctg tgttccgggt tgaaatattt cgggttgtac aacaatccta
     1141 catccacccc tcgaaggtcc ggtgaatcat agtgaacgat cttgaggttt cgttccttaa
     1201 gtttgggctg actgatcaga tcctgcagca cccggtcgtt ctcgatctcg gcacatccca
     1261 ggattgccca gccgtcgggc gatttgtcgg taccgacctg ggacaacact tccgagagtt
     1321 ttccgagttt atcctggtaa acttccgatg tgtaatgctt gtttcctccc ggaaggaatt
     1381 cttcatcgct cttgttcgga tcgtcgatcg tatcgtacag gttctcgagg ttgtaaaagg
     1441 cgatgatcga caccttatac tgatgcgatt gtgcgcatgc gatactcgtc caggaaagga
     1501 ggatcgcgct gatgaggagt ttgttgattt tcatctttta agggccggca aacttacaaa
     1561 aaccaaaccg cggttccgtt aaactaagct gaagattaaa attggccggt ttaaccttga
     1621 aaacgccatt attcttgtaa ataatcaaga attaacctaa ccatttgaac cctcgcggct
     1681 taattcgtta gctttgccgc ccctccgccg gag
//
LOCUS       DAOTON010000672         1710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_54784_length_1710_cov_3.686405, whole genome shotgun sequence.
ACCESSION   DAOTON010000672 DAOTON010000000
VERSION     DAOTON010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54784_length_1710_cov_3.686405"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /gene="ribA"
                     /locus_tag="P5292_11575"
     CDS             <1..811
                     /gene="ribA"
                     /locus_tag="P5292_11575"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HRS39811.1"
                     /translation="QSKGVLRRSGHTEATIDLARLAGCYPAGVLVEIMNADGSMARLP
                     DLFLIAEKFELPIVSIEDLIAYRLRHETLIERQVDVQLPTVFGDFELIAYGQLNNGQE
                     HLALRKGSWSKDEPVLVRVHSSCMTGDIFGSCRCDCGPQLHKAMELIEADGKGVIVYM
                     NQEGRGIGLLNKLKAYKLQEQGRDTVEANLELGFNSDERDYGVGAQILRDLGVTKMRL
                     MTNNPKKRAGLIGYGLEIVEVVPLQIESNEHNRLYLQTKRDKMGHTIRIDS"
     gene            complement(816..>1710)
                     /locus_tag="P5292_11580"
     CDS             complement(816..>1710)
                     /locus_tag="P5292_11580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRS39812.1"
                     /translation="TILKESAFDIVQLETLFAAPYLPVIRENSQARIVLRAHNVEHII
                     WKRLRDAEHRNPLKRWYLGFLSRRLKREELRIFREVDAIVPITDVDEGMIRQVVPHKQ
                     LLTVPLGVDLNEYPVQDTTGEPLRLFHLGSMDWLPNLEAVNWFLGNCWPRIHQQFPQL
                     ELFLAGRGFPESLLLNAPAGVQCSGTVEDAQGYMRDKQIMVVPLLSGSGMRVKIIQGM
                     ALGKTIISTSIGAEGIGVKDGEHLLIADTPSMFLEKIRYCLEHPEKCRSIGKAARRLV
                     EERYSCASLGKRLIEFYQGLA"
BASE COUNT          397 a          462 c          469 g          382 t
ORIGIN      
        1 acaatcgaaa ggtgtgcttc gccgttccgg tcataccgaa gcaaccatcg atctcgccag
       61 attggctggc tgctatcccg ccggcgtact cgttgaaatc atgaatgccg acgggtcgat
      121 ggctcgctta cccgacctct ttctgatagc cgaaaagttc gaattgccga tcgtatccat
      181 cgaagatctg atcgcgtacc ggctgcggca cgagacgctc atcgagcgac aggtggatgt
      241 tcagcttcca acggtattcg gtgactttga acttatcgcc tacggtcagt tgaacaacgg
      301 acaggagcac ctggccctgc ggaaaggaag ctggtcgaag gacgagcccg tactcgtgcg
      361 tgttcattcg agttgtatga ccggcgacat tttcgggtct tgccgctgtg attgcggtcc
      421 gcagttgcac aaagcgatgg aactgatcga ggcggacggc aaaggggtga tcgtctacat
      481 gaaccaggaa ggtcgtggga tcggtctcct caacaaactc aaagcctaca aattgcagga
      541 gcagggccgt gacacggtcg aagcgaacct ggagttgggt tttaacagcg acgagcgcga
      601 ctatggagtc ggtgcgcaga ttcttcgcga cctgggtgtc accaagatgc gactcatgac
      661 gaataatccg aaaaagcgtg ccggcctgat cggctacggt ctggaaatcg tagaagtcgt
      721 tcccttgcag atcgaatcaa acgagcacaa tcggctttat ctgcagacga agcgggacaa
      781 aatggggcat acgatccgga tcgattcctg acggatcagg ccaatccctg atagaattca
      841 atcagccgtt ttccgagtga tgcgcaactg tagcgctctt ccacaaggcg acgtgccgcc
      901 tttccgatgg atctgcattt ttccggatgt tccaggcaat agcggatctt ttccaggaac
      961 atcgaaggcg tatcggcaat cagcagatgt tccccgtctt ttacgccgat tccttctgca
     1021 ccgatggatg tggagataat cgtttttccc agcgccatcc cctgaatgat cttgacacgc
     1081 atgccggaac ctgataacag tggaacaacc atgatctgct tatcgcgcat gtagccttgt
     1141 gcatcttcta ccgtcccgga gcattgaaca ccggcaggcg cattcaagag cagggattcc
     1201 ggaaaaccgc gaccggcaag aaatagttcc agttgcggaa attgttgatg tatgcgtggc
     1261 cagcaattcc ccaggaacca attgaccgct tcgaggttcg gaagccagtc catgcttccc
     1321 aggtgaaaca accgtagcgg ttcaccggta gtatcctgca ccgggtattc attcaggtcg
     1381 acacccaggg gcacggtcag gagttgcttg tgcggtacca cctgtcggat catcccttca
     1441 tccacatcgg tgatcggaac gatggcatcc acttcccgga aaattctcag ttcctcacgc
     1501 ttcaacctgc gggaaaggaa gccgaggtac cagcgcttca gcggattccg atgctcggca
     1561 tcccggagcc gtttccagat gatgtgctcc acgttgtgcg cgcgcaggac gatacgtgcc
     1621 tgactgtttt cgcggatcac cggaaggtag ggcgcagcaa aaagtgtttc aagctgcacg
     1681 atatcgaacg cactttcttt cagaatcgtc
//
LOCUS       DAOTON010000673         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_54837_length_1709_cov_3.129383, whole genome shotgun sequence.
ACCESSION   DAOTON010000673 DAOTON010000000
VERSION     DAOTON010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54837_length_1709_cov_3.129383"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="P5292_11585"
     CDS             <1..694
                     /locus_tag="P5292_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS39813.1"
                     /translation="PLRANNGSSLASKLGLDAFVLFKDRKMAIFFFFSMCLGAALQIT
                     NAFGGAFLDSFQSTFPDTFAVQHPILLLSISQISETLFILTIPFFLKKFGIKKVMLMS
                     MFAWVFRFGLFSIGNPGSGLPLLILSMIIYGMAFDFFNISGSLFVEKEAPQSMRASAQ
                     GLFMFMTNGIGAMFGGYGSGLVVDHYTLNGVSDWPSIWMVFTIYALILGLIFPFAFKY
                     KHETGAVTEVRH"
     gene            complement(759..1361)
                     /locus_tag="P5292_11590"
     CDS             complement(759..1361)
                     /locus_tag="P5292_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HRS39814.1"
                     /translation="MKKIKLDIIGLSYSQTQSGAYALVLGEEGGKRRLPIIIGAFEAQ
                     AIAIELENMTPTRPLTHDLFKSMAGSFDIDVEEVIIFNLLEGVFYAKLICNNGEKRME
                     IDARTSDAIAIAVRFGCPIYTYEFILSTAGIIVDESAEGGEEQQVVEKPAVTTTKLSE
                     GELSAASTDELNEMLTKALEEEAYERASRIRDELNKRKSN"
     gene            complement(1438..>1709)
                     /locus_tag="P5292_11595"
     CDS             complement(1438..>1709)
                     /locus_tag="P5292_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRS39815.1"
                     /translation="PVSDAGWRPHSEHVGQTGIAISPNLYIAVGISGAIQHLAGVSSS
                     KVIVVVNKDPEAPFFKAADYGICGDAFEVVPKLIEAAKTFKASQN"
BASE COUNT          342 a          488 c          428 g          451 t
ORIGIN      
        1 gcccttgcgg gcgaacaacg gttcctcgct tgccagcaag ctcggtctgg acgcattcgt
       61 cctcttcaaa gaccggaaga tggcgatctt cttcttcttt tccatgtgcc ttggtgccgc
      121 cttgcagatc accaacgctt tcggcggtgc cttcctcgac agcttccagt cgaccttccc
      181 cgacaccttc gccgttcaac atccgatcct cttgctctcc atttcgcaga tctccgaaac
      241 actgttcatc ctgaccatac ctttcttcct gaagaaattc ggcattaaga aggtcatgtt
      301 gatgagtatg ttcgcctggg ttttccgctt cggcctattc tccatcggca atcccggcag
      361 cggacttccc ttgctgattc tatcgatgat catctacggc atggcgttcg acttcttcaa
      421 catctccggg tcgttattcg tcgagaaaga agcgccgcag agcatgcgcg ccagcgctca
      481 gggactattc atgttcatga ccaacggcat cggcgcgatg ttcggcggct atggaagcgg
      541 actcgtggtg gatcattata cgttgaacgg cgtcagcgac tggcccagca tctggatggt
      601 tttcaccatt tatgccctga tcctcggact gatctttcca ttcgccttca agtacaagca
      661 cgaaacgggt gccgttaccg aagtccgtca ctgaaccgct tcatcgaaat aaaaaaagca
      721 gcctgtccgg gctgcttttt cattttcatg tatgctgatc agttgctctt tcgtttgttc
      781 aactcgtccc ggatacggga ggcgcgttcg taggcttcct cttcgagcgc tttcgtgagc
      841 atctcgttca attcgtccgt gctggctgcg gacaattcgc cttccgacaa tttcgtggtg
      901 gtcaccgcag gtttctccac tacctgttgc tcttctccgc cttcagcact ctcgtcaacg
      961 atgatgccgg cggtagagag gatgaattcg tatgtgtaga tcggacagcc gaagcgaacc
     1021 gcgatggcga tggcgtcgct ggtgcgggca tcgatctcca tccgtttttc cccgttgttg
     1081 caaatgagct ttgcatagaa cacgccttcg agcaggttga agatgatgac ctcttcgacg
     1141 tcgatgtcga acgatccggc catggacttg aacagatcgt gggtcaacgg ccgggtgggc
     1201 gtcatgtttt cgagttcgat cgcgattgcc tgcgcttcga acgcgccgat gatgatcggc
     1261 aaccggcgct tccctccttc ttcacccaat accaaagcat aggccccgct ttgcgtctgg
     1321 ctgtagctga ggccgatgat gtccagtttt atttttttca tagaaaagct ggagctggta
     1381 ccccggcatt acctaaacga atataagaaa attctcctga gttctgggtg aaacgaatca
     1441 attctggctt gctttgaacg tcttggcagc ctcgatgagc ttaggcacta cttcgaacgc
     1501 gtctccgcaa attccataat ccgccgcttt gaagaacggg gcttcaggat ccttattgac
     1561 aacaacaatc actttcgacg aactgactcc ggccaaatgc tggatggctc cggatatacc
     1621 gacagcaatg tacagattcg gcgagatggc gataccggtc tgccccacgt gctcactgtg
     1681 cggacgccag ccggcgtcgg aaaccggtt
//
LOCUS       DAOTON010000674         1708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_54887_length_1708_cov_3.033273, whole genome shotgun sequence.
ACCESSION   DAOTON010000674 DAOTON010000000
VERSION     DAOTON010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54887_length_1708_cov_3.033273"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1034
                     /locus_tag="P5292_11600"
     CDS             <1..>1034
                     /locus_tag="P5292_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39816.1"
                     /translation="PMYQPNMQLSDAGVYTVTITSPNGCTTTRSVNVVVNPSPVAPVI
                     QSNPGGILCEGSLFNLFVTNPAGAGHTYNWSTGQLGLSINAALVGDYWVMVTNSFGCK
                     ANSNILTIHPRPDLSCAPTGCYEFCNECDSVTIPGPAGMATYTWQIFVPNMFVYYSNS
                     QNLTVLPPGGKFNLVATNLWGCADTTDTLNIHFEDCCPAPDLFTCLDTCENFDDNLLN
                     DFGPDPNASNVIVSTSNVNSQGGPGDYYLFVQDQPGASGVLGSYLFDGKWCCGSFCFD
                     FRLLNDGGVGGNVNPSVTIMSGTLGFTFTSTINANQNNGWHSVCAPITDCNPAPAGPG
                     GAWTPVGATL"
     assembly_gap    1035..1044
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1045..>1708
                     /locus_tag="P5292_11605"
     CDS             <1045..>1708
                     /locus_tag="P5292_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39817.1"
                     /translation="TVLSNVTEVIFPADYNAAPDEASALDNICLNSDVPDIDAGPDTT
                     ICVGSVVTLHVSGCTGTPTWSQISGDSLIPLNNGPFIDVMPLQNTCYVVTCCGYQACC
                     CDTDTVCVNVRPLPQLQWNLPFTPVCLGSDSIYLDTANISVFVNNNWIPLANAGGTWV
                     FSGANVIGNYFYPNLLGPNAVTLTYTDSAGCSASLTKYIGVIFCCPDTCEADAGPDHV
                     VCL"
BASE COUNT          349 a          451 c          456 g          442 t
ORIGIN      
        1 cccgatgtat caaccgaaca tgcaattgtc ggatgcggga gtgtatacgg tgaccatcac
       61 cagcccgaac ggttgtacga caacacgatc ggtgaatgtc gtggtgaatc cgagtccggt
      121 tgctccggtc attcaatcga atccaggcgg tatcctgtgt gagggcagct tgttcaatct
      181 ctttgtaacc aatcctgcag gcgcagggca cacttataac tggagtaccg gacaactcgg
      241 cctgagtatc aatgctgctt tggtgggcga ctattgggta atggtcacca actcattcgg
      301 ttgtaaagcc aacagcaaca tcctgaccat tcatccgcgt ccggatctga gttgcgcgcc
      361 aaccggatgt tatgaattct gcaacgagtg tgattcggtt acgattcccg gtcccgccgg
      421 catggcaacc tacacctggc agatcttcgt gccgaacatg ttcgtatact acagcaacag
      481 ccagaatctg accgtgcttc ctccgggcgg aaagttcaac ctggtggcta cgaacctatg
      541 gggttgcgcg gatactacgg atacgctcaa cattcacttt gaagattgtt gtcctgctcc
      601 ggacctgttt acctgtctcg atacctgtga gaatttcgac gataacctgc tgaacgattt
      661 cggtcctgat ccgaatgcat cgaatgtgat cgtctccact tcgaatgtca attcgcaggg
      721 cgggccgggt gattattatc tgttcgtaca ggaccagccc ggcgcttcgg gtgtgttggg
      781 cagttatctg ttcgacggga aatggtgttg cggaagtttc tgttttgatt tccggttgtt
      841 gaacgacggc ggagtgggtg gtaacgtgaa tccttccgtt accatcatga gcggcacgct
      901 cggtttcacc tttacttcta cgatcaatgc caatcagaac aacggctggc attcggtctg
      961 tgcgccgatc acggattgca atcctgctcc tgccggaccc ggcggagcct ggacaccggt
     1021 aggagcgacc ttgcnnnnnn nnnnaaccgt cttgtcgaac gttacggaag tgatcttccc
     1081 cgcggattac aatgccgcgc cggatgaagc gagcgcgctc gacaatatct gtctcaattc
     1141 ggatgtgccc gatatcgatg caggtccgga tactacgatt tgtgtcggtt cggttgtcac
     1201 cctgcacgta agcggttgta cgggtacacc gacctggtcg cagatctccg gtgattcttt
     1261 gatcccattg aacaatggtc cgttcatcga tgtcatgccc ttgcaaaata cctgctatgt
     1321 cgttacctgt tgtggctacc aggcttgttg ttgtgatacc gatacggttt gtgtcaacgt
     1381 acggccgctc ccgcaactgc aatggaatct tccgttcacc cccgtatgtc tgggaagtga
     1441 ttcgatctac cttgatacgg cgaatatttc ggtatttgtg aacaacaatt ggatcccgct
     1501 cgccaacgca ggtggcacct gggtattcag cggcgccaat gtgatcggaa attatttcta
     1561 cccgaacctg ctcgggccga atgctgtcac gttgacctat acggattctg ccggctgcag
     1621 cgcgagcctg accaagtaca tcggcgtgat tttctgttgt cccgatacct gcgaggcgga
     1681 tgccggcccg gatcatgttg tctgcctt
//
LOCUS       DAOTON010000675         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_55001_length_1706_cov_3.221078, whole genome shotgun sequence.
ACCESSION   DAOTON010000675 DAOTON010000000
VERSION     DAOTON010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55001_length_1706_cov_3.221078"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="P5292_11610"
     CDS             complement(<1..981)
                     /locus_tag="P5292_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HRS39818.1"
                     /translation="MHLIFFDDENRQRLLPLTFTRPVAEIRLGILTLREKWERRLGAG
                     GSSFRTADYLRVKYGFAATDDNLWINGSVIADDALVSAVQQLEKGSCLCAGEVVIAAR
                     TDQAAAKDGGSDPDFSAMKPVEYGGSLEQVRYPWDIFSKNGKGIEADFALLTKGRKSA
                     PLSASNRVIAPERVFLEPGAKAECAILNASAGPIYIGKDAEVMEGAMIRGPFALCEHS
                     TVKMGAKIYEGTTFGPECKVGGEVSNSVIFGYSNKAHDGFLGNSVLGEWCNLGADSNN
                     SNLKNNYSMVKVWSYEEETYVNTRLQFCGLIMGDHSKCSINTMFNTGTVVG"
     gene            complement(1020..1400)
                     /locus_tag="P5292_11615"
     CDS             complement(1020..1400)
                     /locus_tag="P5292_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39819.1"
                     /translation="MTKVRVLLLALVLTFLCGYMEWGGGQSITLAQAEYQLFFQQPVS
                     SDSMTHPLILLPFAGQVLLLANLFLAKPRRRLGTFGLVLMGVLALMILLVGILSRNIW
                     MVLSILPFLALAVLHFRKPDWYPA"
     gene            complement(1487..>1706)
                     /locus_tag="P5292_11620"
     CDS             complement(1487..>1706)
                     /locus_tag="P5292_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRS39820.1"
                     /translation="YRMVVFKDISCDYAFLTRSTVETKDTIKWTDGNEYPLVKLEISH
                     MSHPFYTGKMKLVDTAGRIDKFRNRYKK"
BASE COUNT          350 a          514 c          492 g          350 t
ORIGIN      
        1 gcccaccacc gttccggtgt tgaacatggt gttgatgctg cacttggaat ggtcgcccat
       61 gatcagcccg cagaactgca gccgggtgtt cacgtaggtc tcctcctcgt agctccacac
      121 tttcaccatc gagtagttgt tcttcaggtt ggagttgttc gagtcggcgc cgaggttgca
      181 ccattcgccc aacaccgaat tcccgaggaa gccgtcgtgc gccttgttcg aatagccgaa
      241 gatcaccgag ttgctcacct cgccgccgac cttgcactcc ggtccgaagg tcgtgccttc
      301 gtagatcttc gcgcccatct tcaccgtcga atgttcgcag agggcgaacg gcccccggat
      361 catcgcgcct tccatcactt cggcgtcctt gccgatatag atcggtccgg cgctggcgtt
      421 caggatcgcg cactccgctt tcgcgccggg ctccaggaag acgcgctccg gcgcgatcac
      481 gcggttcgat gccgacagcg gcgccgactt cctgcccttc gtgagcaggg cgaaatcggc
      541 ttcgatgccc ttgccgttct tcgaaaagat atcccaggga tagcgtacct gttccaggct
      601 gccgccatac tccaccggct tcatggcgga aaaatcagga tcgctgccgc cgtctttggc
      661 ggccgcttga tcggtacgcg cggcgatcac cacttcgccg gcacacaggc aactgccttt
      721 ttccagttgc tgcacggcgc tcaccagcgc atcatcggcg atcaccgagc cgttgatcca
      781 gaggttgtca tcggtcgcgg cgaagccgta cttcacgcgc aggtagtcgg ctgtacgaaa
      841 cgaactgccg cccgcgccga gccggcgttc ccacttttca cgcagcgtca ggatgcccag
      901 ccggatctcg gccaccggcc gggtgaaggt cagcggcagc aggcgttgcc ggttttcgtc
      961 gtcgaagaag atgaggtgca tagtgttgtt gaactgcccg atgcttgtaa gctcactact
     1021 caggcaggat accagtcagg tttacggaag tgaagaacag ccagtgccag gaacggcagg
     1081 atcgagagca ccatccaaat gttgcgactc aggataccga ccaacaggat catcagcgcc
     1141 agcacgccca tcagcacaag gccgaaggtg ccgagtcgcc gacgcggctt ggcgaggaag
     1201 aggttggcca gcagcaacac ctgcccggcg aagggcagca ggatgagcgg gtgggtcatg
     1261 gaatccgacg aaacgggctg ttggaagaac aactggtact ccgcctgggc cagcgtgatc
     1321 gattgcccgc cgccccattc catgtatccg cagaggaagg tcagcaccag ggccaggagc
     1381 aatactcgga ctttagtcat cgacggcgat tccggtcaaa tttagccaaa cacaaaagaa
     1441 aaagtccctc ttgcggaggg actttttcaa cgaaaaccga atggttttat ttcttgtagc
     1501 ggttgcggaa cttgtcgata cgaccggccg tgtcgacgag cttcatctta ccggtataga
     1561 acggatgcga catgtggctg atctccagct ttaccagcgg atactcattg ccgtcggtcc
     1621 acttgatggt gtctttcgtc tcgaccgtcg aacgggtcag aaaagcgtag tcgcaggaaa
     1681 tgtccttgaa tacgaccatt cgatag
//
LOCUS       DAOTON010000676         1705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_55054_length_1705_cov_3.348485, whole genome shotgun sequence.
ACCESSION   DAOTON010000676 DAOTON010000000
VERSION     DAOTON010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55054_length_1705_cov_3.348485"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="P5292_11625"
     CDS             complement(<1..293)
                     /locus_tag="P5292_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRS39821.1"
                     /translation="MNNELVIHSTNGEVTIALLEDKQLVELNNDKKDQSFQVGDIYLG
                     RVKKLIPGLNAAFVDVGHEKDAFLHYLDLGPQAQSLLKLVRLATTGKNPDPLLT"
     gene            complement(626..1456)
                     /locus_tag="P5292_11630"
     CDS             complement(626..1456)
                     /locus_tag="P5292_11630"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS39822.1"
                     /translation="MSARRTQLVLVAGAIALTGVLYLLPRKTSEEKAAIQSQSRQSTY
                     TFEGLLADAKSQVKRQELDPINQLEDKLQQAPQDTAVLGELGRSWDRLGFPAISSGYF
                     RRIAEAAPGENTWINAAYRYFDAFKLSGDSTLQSMMVQQAIESYGKVLAINPDNLDAK
                     TDLGICYAEGTTTPMQGIMLLREVVEKNPGHANAQFNLGILSVRSGQYDKALERFSKV
                     LEIDPKRTLARFMIGRVYAQQGQQEKALAALEQVKKESSDPQLLKEADQLINQIQNNH
                     "
     gene            complement(1462..>1705)
                     /locus_tag="P5292_11635"
     CDS             complement(1462..>1705)
                     /locus_tag="P5292_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRS39823.1"
                     /translation="VEAAMKVIKNTMADGENVYLRGFGSFIVKKRAQKLGRIISKNTT
                     IVIPEHNIPSFKPAKTFANKVKNAKIKKNQTSEAID"
BASE COUNT          318 a          459 c          482 g          446 t
ORIGIN      
        1 gtcagcagtg ggtccgggtt cttcccggtg gtagccagtc gtaccagttt cagcagcgac
       61 tgggcctgag ggccgaggtc gaggtaatgc aagaacgcgt ccttttcgtg acctacgtcg
      121 acgaatgccg cattcaaacc gggaatcagt ttcttgacac gtcccaggta aatgtcaccg
      181 acctggaacg actgatcctt tttgtcgttg ttcagttcaa ccagttgctt gtcttccagc
      241 agggcgatgg tgacttcgcc attggttgaa tgaatcacca attcgttgtt caacgaaata
      301 tgttttagcg acccgattgg cgcgatggcc gaggggtaat ccgcagaaaa acaccgaaca
      361 gccggaactg ttcacgcatc cgtttgcagg gaccggttgg agtgccggcg acgcatcgcg
      421 cggcgaaggg agtgattcga aaaggccctg ttttcccgtg ggaaaagtag gatacggaaa
      481 acgggcgcgg aagcgttcac ttccgcgccc ggcagtatta ccggttcttc ttcttatgtc
      541 tgttctttct caggcgtttt ttacgcttat gcgtcgccat cttgtgtctt tttctcttct
      601 taccgcttgg catagtccta cgggtttagt ggttattttg aatctggttt attaactgat
      661 ccgcctcttt caacagctgc ggatccgaac tttctttttt cacctgttcc agcgcggcga
      721 gcgctttttc ctgctgcccc tgctgggcgt agaccctgcc gatcatgaaa cgcgccagtg
      781 ttcgtttcgg atcgatctcc agcaccttgg aaaagcgctc gagggccttg tcgtattgtc
      841 ccgaccttac cgaaaggatc ccgaggttga actgggcgtt ggcgtgcccg ggattctttt
      901 cgaccacttc gcgcaagagc atgatgcctt gcatcggcgt ggtggttcct tcggcataac
      961 agataccgag gtcggttttg gcgtcgaggt tgtcgggatt gatcgcgagg acctttccat
     1021 acgactcgat cgcctgctgc accatcatgg actggagcgt ggaatcgccc gagagcttga
     1081 aggcatcgaa gtagcggtag gctgcgttga tccaggtgtt ctcacccggc gccgcttcgg
     1141 ctatccggcg gaaatatccc gaagagatgg ccggaaatcc gagccggtcc caggaccttc
     1201 ccaactcgcc cagcaccgcg gtatcctgcg gagcctgttg cagtttgtct tccaactggt
     1261 tgatcgggtc cagttcctgc cgcttcacct gcgacttcgc gtcggccagg aggccttcga
     1321 aagtgtaggt ggattgacgg ctctgcgact ggatcgccgc tttctcttcg gaagtcttgc
     1381 gtggaagaag atacaagact ccggtcagtg cgatggcacc cgctaccagg accaactgtg
     1441 tacggcgggc actcatcggg atcaatcgat cgcttcggag gtttggttct tcttgatctt
     1501 ggcgttcttc accttgttgg caaaagtctt ggccggcttg aaggacggaa tgttatgctc
     1561 cgggatcacg atggtggtgt tcttggaaat gatgcggccc agtttctgag cacgcttctt
     1621 cacgatgaac gaaccgaaac cgcgcaggta aacgttctcg ccatctgcca tggtgttctt
     1681 gatcaccttc atggctgcct ctact
//
LOCUS       DAOTON010000677         1700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_55294_length_1700_cov_3.644985, whole genome shotgun sequence.
ACCESSION   DAOTON010000677 DAOTON010000000
VERSION     DAOTON010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55294_length_1700_cov_3.644985"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="P5292_11640"
     CDS             complement(<1..1559)
                     /locus_tag="P5292_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS39824.1"
                     /translation="MNEQVLQKLLSLFVTTGTLVDRVDASRRAAESLVQQQMSRTAAE
                     VFLQKNWGSSLASDLRAELLQSTARELSIRQKYTVFLQLCELVLLDRDAKPAEEDWLD
                     QIQDAFRLGKKERLRCRHFARRDPLKSLGEQEEPNILLLQEHEVTNPLIQSGVHEGLG
                     GRLWVLYIAGTRLLYLRYEGLENLSAGGSPLPENTIVALSQGTVIRGGRMKPLYFSDL
                     RRRFLVGEAENRIQYLADEVRFLFPGGKTGLHPFRFEVTGGNLVGVMGGSGAGKSTLL
                     SILNGTAEPAEGAIRINGLDLYADARKVQGLIGNIPQDDLLIEELTVYQNLYYNTKLC
                     FGQLDSSEIDRKVSDMLQALGLSETRDLKVGNALNKTISGGQRKRLNIALELIREPSI
                     LFVDEPTSGLSSRDAENVMDLLKQLALNGKLVFVVIHQPSSEIFKLFDRLLLLDRGGY
                     PVYDGNPVESVAWFRRLSGQADADDSECATCGHLNPEEIFNILEAPVLDDAGFATQQR
                     KVQPEEWYAPYR"
BASE COUNT          364 a          522 c          439 g          375 t
ORIGIN      
        1 cggtagggtg cgtaccactc ttccggttgc accttccgct gttgcgtcgc gaagcccgcg
       61 tcatccagta ccggcgcttc gagtatgttg aagatctctt ccggattgag gtgaccacaa
      121 gtggcacact cactatcgtc cgcatcggct tgtcccgaca aacgccggaa ccaggcaacg
      181 gactctaccg gattaccgtc gtaaaccgga tatcctccgc gatccaacag caacagccgg
      241 tcgaacaatt tgaatatctc cgacgatggc tggtgaatga cgacgaatac cagcttgccg
      301 ttcagggcca gttgcttcaa caggtccatc acgttctccg cgtcccgcga cgacagaccg
      361 gaggtcggct cgtcgacgaa caggatggat ggttcccgga tcagctccaa cgcgatgttg
      421 agccgcttgc gttgcccgcc gctgatcgtc ttattcagcg cattgcccac tttcaggtcc
      481 cgggtctcgc tgagaccgag cgcctgcaac atatcgctca ctttccggtc gatctcgctg
      541 gaatcgagct gaccgaaaca taacttcgta ttgtaataga gattctggta aacagtaagt
      601 tcttcgatca gcaaatcgtc ctgcgggatg tttccgatca atccctgaac cttgcgggca
      661 tccgcgtaga gatccagtcc gttgatgcgg atcgcgcctt ccgcgggctc ggctgtcccg
      721 ttcaggatcg acaacaaagt cgacttaccc gcgccactgc cacccattac accgaccagg
      781 ttccctccgg tcacttcgaa gcggaaggga tgcagaccgg tcttgccgcc cgggaacagg
      841 aaccgaactt cgtctgcgag atattgaatg cggttttccg cttcgcccac cagaaacctt
      901 cttctcaaat cggagaagta gagcggtttc atcctgccgc cccgtatcac ggtcccttgc
      961 gacagcgcga cgatggtatt ttcaggaagc ggacttcccc ctgcggagag attttccagg
     1021 ccctcatacc gcaggtacag caagcgcgtt ccggcgatgt acaggaccca gagtcgcccg
     1081 cccagtcctt cgtggactcc tgactgaatc agcggattgg tcacctcatg ctcctgcaag
     1141 agcaggatgt tgggttcttc ctgctcgccc agcgacttca acggatcccg cctggcgaaa
     1201 tgccggcagc gcaaccgctc tttctttcca aggcgaaaag catcctggat ctgatcaagc
     1261 cagtcttcct ccgccggctt tgcatcccgg tcgagtagta ccaactcaca gagttgcagg
     1321 aagacggtat acttctgacg gatactcagt tcgcgagccg tcgattgcag taattccgcc
     1381 cgcagatccg acgcgagcga ggagccccag ttcttttgca gaaatacttc cgcggccgtc
     1441 cggctcatct gctgttgtac cagcgattcc gccgcacgac gcgatgcgtc cacccggtct
     1501 accagggtac cggtggtgac aaaaagcgaa agtagctttt ggagtacctg ctcattcata
     1561 caaccaattc agaaggatgc ctaaaatagg caattcccgt cactaacgaa ttgtcgaaaa
     1621 caagaacggc cagaaccttg cgtcctggcc gttcagacta tcgagatggt tccttaattg
     1681 gtcatgcggt tgcggacctt
//
LOCUS       DAOTON010000678         1698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_55391_length_1698_cov_2.622642, whole genome shotgun sequence.
ACCESSION   DAOTON010000678 DAOTON010000000
VERSION     DAOTON010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55391_length_1698_cov_2.622642"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1698)
                     /locus_tag="P5292_11645"
     CDS             complement(<1..>1698)
                     /locus_tag="P5292_11645"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRS39825.1"
                     /translation="WAGTLGEGVFRLDPGSGKVRQVTEIPELQNASILSITGRGEDLW
                     VTGFGGVNHLKLAFHDGKVEYASVPEPALEILTGIYIYSSHLDRQGALWLGSDEHGLY
                     RYANGSLKQYTQAAGLPGNSVLGICEDAAGVLWVNCIDGGVAALRQDSLHAYGISDGL
                     TDLSLSAILALPDTSLLVVHANGLDRFDPATGTFSPFRPDELMNDLNPDPNTLCLDQA
                     GNVWLGTEKGLLVLEPPNPGDRPFPPTALEHIIQYPDPVNFWERRRFAYDENNFRFEF
                     SGIWYADPGRVQYAIQLEGLSDKWQFSHEPAAWFSNLAPGSYTFRVRTSLNNRFLRSP
                     EYIYHFVVEPPFWKTWWFRILLATGIVGGIWWFVQRRERQLREIERYEKERIMFQLET
                     LRSQVNPHFLFNSFNTLLNLIEKDPKKAAEYTEQLSDFFRDIISNRDLPQVRLEDELK
                     LLSNYIAIQKKRYGDNLNVRIEVEPEHARAFLIPPMTLQLLAENAIKHNVVSRDLPLN
                     ITIALAGNSLCVTNNLQPRLQHEPSTGLGLENIKRRFSLMIEEPVRIEQTAHEFRVCL
                     "
BASE COUNT          387 a          440 c          462 g          409 t
ORIGIN      
        1 caaacataca cgaaattcat gcgcagtctg ctctatccgt acgggctctt cgatcatgag
       61 actgaatcgc cgtttgatgt tttccaggcc caggccggtg gaaggctcgt gttggagacg
      121 tggctgcagg ttgttggtta cacaaagtga atttcctgcc aatgctatcg taatgttcaa
      181 cggcagatca cgcgaaacga cattgtgctt gatagcgttt tcggcaagca gttgcagggt
      241 catgggtgga ataagaaagg ccctggcatg ctccggttcc acctcgatac ggacattcag
      301 attatcaccg tatcgtttct tctggatcgc gatgtaattc gacaacaatt tcaattcatc
      361 ttccagcctg acttgcggca ggtcgcggtt ggaaatgatg tcacggaaaa aatcagacag
      421 ttgctccgtg tattcggccg ctttctttgg atctttttca atcaggttca acagggtgtt
      481 gaagctgtta aaaagaaagt gcgggttgac ctggctgcgc agggtctcga gttggaacat
      541 gatacgttcc ttttcatagc gttcgatctc ccgcaattgc cgttcgcgac gctggacgaa
      601 ccaccagatg cccccgacaa ttccggtggc cagcaggatg cggaaccacc aggtcttcca
      661 gaacggaggt tcgaccacga agtgataaat gtattccggc gaacgcagga agcgattgtt
      721 cagcgatgtc cggactcgga acgtatagct gcccggtgca agattactga accaggctgc
      781 cggttcatgg ctgaattgcc atttgtcact caatccttcg agctgtatgg catattggac
      841 tctcccggga tcggcatacc agatgccgga aaactcaaag cgaaaattgt tttcatcata
      901 ggcaaatcgt cgccgctccc aaaagttgac cggatcggga tattgtatga tgtgttccag
      961 cgcggtggga gggaacggtc ggtcgcccgg gttcggtggt tcaaggacca acagtccctt
     1021 ttccgtgccc agccaaacat tccccgcctg atcaagacaa agcgtattgg ggtcaggatt
     1081 cagatcgttc attaattcgt ctggccggaa gggggagaag gtgccggtcg cgggatcgaa
     1141 gcgatccagc ccgttggcat gtacaacaag cagcgatgtg tcgggaaggg ctaagatggc
     1201 acttaggctt aggtcggtaa gaccgtccga tataccatat gcatgcaaac tatcctggcg
     1261 tagggccgct actccgccat cgatgcaatt gacccagagg acgccggcag cgtcttcaca
     1321 tattcccaaa acgctattgc cgggaagacc cgctgcctgt gtgtactgct tgaggctccc
     1381 gttcgcatat cggtacaatc cgtgttcatc gctgccgagc cataaggcac cctgacgatc
     1441 gaggtgactg ctgtagatat aaatcccggt caggatttcg agcgctggtt ccggaacgga
     1501 tgcgtactcc actttcccat cgtgaaaagc cagtttcaaa tggttcacac cgccgaatcc
     1561 ggtgacccaa aggtcttcgc ctcttcccgt gatggagagg atactggcat tctgcaattc
     1621 agggatttcg gttacctgcc ggacctttcc gcttccggga tcgagtcgga agacgccttc
     1681 tccaagtgtt ccggccca
//
LOCUS       DAOTON010000679         1693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_55633_length_1693_cov_3.014652, whole genome shotgun sequence.
ACCESSION   DAOTON010000679 DAOTON010000000
VERSION     DAOTON010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55633_length_1693_cov_3.014652"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..334)
                     /gene="rpmI"
                     /locus_tag="P5292_11650"
     CDS             complement(137..334)
                     /gene="rpmI"
                     /locus_tag="P5292_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRS39826.1"
                     /translation="MPKVKTNSSAKKRFKITGTGKIKRKHAYKSHILTKKETKRKRNL
                     THAALVSPSDENRVLSMLGMR"
     gene            complement(391..915)
                     /gene="infC"
                     /locus_tag="P5292_11655"
     CDS             complement(391..915)
                     /gene="infC"
                     /locus_tag="P5292_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRS39827.1"
                     /translation="MKREPEHKINSFIRAPKVRLVGDNVTPDIYSLEDALKIAQEIGL
                     DLVEVVPTADPPVCRIVEYQKFLYEKKKKDKELKSKQGKQVVKEIRFGPQTDDHDFNF
                     KVKHAIKFLEEGNKVKAYVHFRGRAIAYKEQGELILLKFAQALEEYGKVELMPKLEGN
                     RMFLHLAPALKKKK"
     gene            complement(1025..>1693)
                     /locus_tag="P5292_11660"
     CDS             complement(1025..>1693)
                     /locus_tag="P5292_11660"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRS39828.1"
                     /translation="HEPKKLGVKYVDNPELWLQTEDMVRQVLIDSKIPYVEVPDEGAF
                     YGPKIDVQVWSAIGREFTLATNQVDFAVPARFGLTYRNSNNEFETPICIHRAPLGTHE
                     RFIGFLIEHFAGNFPIWLAPKQVAVLPISDKYLDYAKKVLQFLRNADIRGLLDDRDEK
                     IGKKIRDSELKKVPYMLIVGEKEAAENKVSVRKHGEGDTGSVNIADFIQSVQDEINKC
                     LELN"
BASE COUNT          330 a          421 c          451 g          491 t
ORIGIN      
        1 attttctttc tgcggttgcg cgaggcaacg tggttaactg aacgtggcat tgattttcgg
       61 tttttgactg gagcggccgc tcaaacgaca ctttggggct ctttgtctgg ttgttgtttt
      121 tacggttgtg gcaggatcaa cgcatgccaa gcatgctcaa tacgcgattc tcatccgacg
      181 gggagacgag ggcggcgtgg gtgaggttcc gcttccgctt ggtctccttc ttggtcagga
      241 tgtgactctt gtaggcatgc ttgcgcttga tcttacccgt accggtgatc ttgaaacgct
      301 tcttggcgct cgagttcgtt tttactttcg gcatgggaag ttgtttattg ggtttatagg
      361 gtaatgcttt gttggtgcag ttcgaaccgc ttatttcttc ttcttgaggg caggggcgag
      421 gtggaggaac atccggttgc cttccagttt cggcatcagt tccacttttc cgtactcctc
      481 cagcgcctgg gcgaacttga gcaggatgag ttctccctgt tctttgtaag cgatggcacg
      541 gccgcggaag tggacgtacg ctttcacctt attgccttct tcgaggaact tgatcgcgtg
      601 cttcaccttg aagttgaagt cgtgatcatc cgtctgggga ccaaagcgaa tctctttgac
      661 gacctgcttg ccctgtttgg acttaagttc cttgtctttc tttttcttct cgtaaaggaa
      721 tttctggtat tcgacgattc ggcagacagg aggatcggcg gtaggtacaa cttctaccag
      781 atccaaacca atttcctgcg cgatcttcaa ggcatcctcg agggaataga tgtcgggggt
      841 gacattgtca cctaccagac ggactttcgg agcacggatg aaactgttga tcttgtgctc
      901 aggctcccgc tttatcggac cacgtccgcg aaagaagggc cttcccccgc cgccacctga
      961 cggcctgttg aaacttgaag ttgcgatacc agcctccttt tttagttcgt attcgtatgc
     1021 tattttaatt caattcaagg catttattga tttcatcctg aacggattga atgaaatcag
     1081 caatgttgac gctgccagta tcaccttcac cgtgtttccg gacggaaacc ttgttttcgg
     1141 cagcttcttt ttccccaaca atcagcatat acggaacctt cttcagttcc gagtcgcgga
     1201 tctttttacc gattttttca tcccggtcgt cgaggaggcc gcgaatatcg gcatttcgga
     1261 ggaattgtaa aacttttttc gcgtaatcga ggtacttatc ggagatcggg aggacggcta
     1321 cctgcttggg agccagccaa atagggaaat ttccggcaaa atgctcgatc aggaagccga
     1381 tgaagcgttc atgggtcccc agcggcgccc ggtggataca gatcggggtc tcgaattcgt
     1441 tgttggaatt ccgataggtc aggccgaatc gggccggtac ggcgaagtcg acctggttcg
     1501 ttgccagggt gaattcacgg ccaatcgcgc tccaaacctg cacatcgatc ttcggtccgt
     1561 aaaaagcgcc ctcatccggt acttccacat agggaatctt gctgtcgatc aatacctggc
     1621 ggaccatgtc ctcggtctgc agccagagtt ccgggttgtc gacgtacttc accccgagtt
     1681 ttttcggttc gtg
//
LOCUS       DAOTON010000680         1690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_55775_length_1690_cov_3.053823, whole genome shotgun sequence.
ACCESSION   DAOTON010000680 DAOTON010000000
VERSION     DAOTON010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55775_length_1690_cov_3.053823"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="P5292_11665"
     CDS             complement(<1..876)
                     /locus_tag="P5292_11665"
                     /inference="COORDINATES: protein motif:HMM:NF001133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HRS39829.1"
                     /translation="MQGYNTFDRDTLIRQLEAENQPRTTLSFYRYVNIDDPSALRQRL
                     FTEWPGMGIRGRIYLAKEGVNAQLSLPAARLDEFRAWIDACPGFEQIPFKIAVEDDGK
                     SFLKLIVKVKKKIVADGLDDDAFDTTNVGRHLDAKSWNDALAQPDTIVVDMRNCYESE
                     VGRFPGALCPDVVTFREELPLVLDSLKGKEQSKVLLYCTGGVRCEKASAFLRHHGFED
                     VNQLHGGIIDYTRQVRAEGMENRFIGKNFVFDERMGERISDDVIAHCHQCGKPADTHV
                     NCANEACHVLFIQCSE"
     gene            complement(968..1192)
                     /locus_tag="P5292_11670"
     CDS             complement(968..1192)
                     /locus_tag="P5292_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39830.1"
                     /translation="MKRLAVPFFAIFLLLASSDVSAQCAMCKRNVETNREQGTNKVGK
                     GLNQGILYLMAVPYLMGAIGIYSWMKSRKK"
     gene            complement(1196..>1690)
                     /locus_tag="P5292_11675"
     CDS             complement(1196..>1690)
                     /locus_tag="P5292_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HRS39831.1"
                     /translation="GIFSVVVFALVAVLGRLPKVPDHPAWVEQLPAFNAIINGTCFFL
                     LLASLWFIRKRNIPMHRRLNLLASALSVIFLASYVTFHYFVEETRFPADDPLRPVYLV
                     ILATHILLAAIVLPLVLLSLYRGLTNQVDRHRKIVRWSYPVWLYVTFTGVIVYLMIAP
                     HYRF"
BASE COUNT          417 a          440 c          476 g          357 t
ORIGIN      
        1 ttcggaacac tggataaaaa gcacgtgaca ggcttcgttg gcgcagttca cgtgggtgtc
       61 ggcgggcttt ccgcactggt gacaatgcgc gatgacatcg tcggagatcc gctcgcccat
      121 gcgttcgtcg aagacgaaat tctttccgat gaaacggttc tccataccct ccgccctcac
      181 ctgccgtgtg tagtcgatga tcccgccgtg cagttgattc acgtcttcga aaccatgatg
      241 ccttaaaaaa gcgctggctt tctcacaccg tacaccgccg gtgcaataca acaacacttt
      301 gctttgctcc ttgcctttca ggctatcgag tacgagcggc agttcttcgc ggaaggtgac
      361 gacgtccgga caaagggcgc cgggaaagcg gcccacttcg ctctcgtaac aattgcgcat
      421 gtcgaccacg atggtatcgg gttgcgccag tgcgtcattc caggatttcg cgtcgaggtg
      481 acgaccgacg ttcgtcgtgt cgaatgcgtc atcatcgagg ccgtcggcca cgatcttttt
      541 cttcaccttg acgatcagct tgagaaaaga ctttccgtca tcctccacgg cgatcttgaa
      601 cgggatttgt tcgaagccgg ggcaggcatc gatccaggca cggaattcgt cgagacgcgc
      661 agccggtaac gacaactgtg cattcactcc ctccttcgcc aaataaatcc gaccacgaat
      721 ccccatgccc ggccattcgg tgaacagccg ctggcgcagc gcggaaggat cgtcgatgtt
      781 gacataccgg taaaatgaca gcgtagtacg cggttgattc tccgcttcca actgccggat
      841 cagggtgtcg cgatcgaagg tgttataacc ttgcatgggg ctgcaaaggt acggaactaa
      901 aaaaggtgtg tcaaagacgc ctttttatga taaaaatcaa atttctaaga tcgaattgag
      961 ggcggcgcta ttttttccgg gatttcatcc aggagtaaat cccgatggcg cccatgagat
     1021 agggaacggc catgaggtac agaatgccct ggttcagccc ttttccgacc ttgttggtac
     1081 cctgctcacg attggtttcc acatttcgct tgcacatggc gcattgcgcg gacacatcgg
     1141 atgacgcgag gagcagaaag atggcgaaga agggtactgc gaggcgtttc atggttcaga
     1201 aacgataatg gggcgcgatc atcaggtaga cgatcacacc agtgaaggtt acatacaacc
     1261 agaccggata cgaccagcgg acgattttcc gatgacgatc aacctggttg gtcaatccgc
     1321 gatacaaaga tagcaaaacg agcgggagga caatggccgc caggaggatg tgggtagcaa
     1381 gaatgacgag gtatacgggc cgcagtggat catcggccgg aaagcgcgtt tcttccacga
     1441 agtaatggaa ggtcacgtaa gaagccagga aaatgaccga taaagccgag gccagcaggt
     1501 tcagccggcg atgcatcgga atgttgcgct ttcggataaa ccagagggat gccaacaaga
     1561 ggaaaaagca agtgccgttg atgatcgcat tgaacgccgg cagttgttcg acccaggccg
     1621 gatgatcggg tactttcgga agtcgaccga gtacggctac cagggcaaag acgaccaccg
     1681 agaatatccc
//
LOCUS       DAOTON010000681         1686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_55994_length_1686_cov_2.079706, whole genome shotgun sequence.
ACCESSION   DAOTON010000681 DAOTON010000000
VERSION     DAOTON010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55994_length_1686_cov_2.079706"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="P5292_11680"
     CDS             complement(<1..1539)
                     /locus_tag="P5292_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39832.1"
                     /translation="MLLRTGFKRRILRVLLGCMTLLCHQDASAIIKTSLMSGNWSNPA
                     IWLPTGVPTAADDVQILTTNTVTLDNNFTVNRVTVMDGGTLTFNSGMRLTMTGNFTVN
                     GTCTMNRGDITLSSPGLQFNLGPSSQFTWDPGTNDAVNATLFIRGTENFDPGSVLIIK
                     RWYNYSIPLGGMVSCNFGNLTINTPGPTAGTIAEWNQSNWFQTRRVLGTLTIDQGWIT
                     LDKSGNIGTTSFGSIVLTSVNSTFYGHSGTHPGAFTIQTGSLTNNGGNFFVLQNGNGD
                     AALQVSGNVFNIGNIKIINNSGVAGVSNGNAQLIVNGSFTQNTGDTRIIYNIATTNSG
                     TYTARFGNLILNGGIFFGQTGVHTAGGTNRLDVTGQFTIQFAVSTDKFRGSSLSSIGV
                     AMNNAAFEMTVGGNMSINGPSAAEFTSSAASGNEQVQLNGNLTVAGLTSSFNFGTSAS
                     AHTVNLTVSGAVTISGGVLFLSRNNGPVTSSLGSLAISGGQLNVKGGTGMRVVSVDVD
                     HSGSG"
BASE COUNT          367 a          430 c          473 g          416 t
ORIGIN      
        1 accggaaccg gagtggtcga catcaacgga aactacacgc attccggtcc ctcctttgac
       61 attcaattgt cctccgctga tggcaaggct tccaaggctg gaagtgaccg gaccgttatt
      121 tctggagagg aacagtacac cgccgctgat agtcacagcg cccgacacgg tgagattcac
      181 cgtatgcgcc gaagccgaag tgccgaagtt gaagctcgat gtcagacctg ctacggtgag
      241 atttccgttg agttgaacct gttcgtttcc tgacgctgcg ctactcgtga actccgctgc
      301 cgatggtccg ttgatactca tgttaccacc caccgtcatc tcaaatgctg cgttattcat
      361 ggcgacgcca atcgaagaga gcgaacttcc tctgaattta tccgtcgata ccgcgaactg
      421 gatggtgaat tgtcccgtca catccagccg gttcgtgcca cctgcagtgt gcacgcccgt
      481 ttgtccgaag aaaattcctc cattcagaat caggttcccg aaccgcgcgg tgtaggtgcc
      541 ggagttggtg gtggcgatat tgtagatgat tcgtgtgtca ccggtattct gggtgaaaga
      601 accgtttacg atcaattgag cattgccatt gctgacgccg gctactcccg aattgttaat
      661 gattttgata ttcccgatgt tgaatacatt cccgctgact tgcagagcgg catcaccgtt
      721 tccgttttgc aggacaaaga agttgccgcc gttgttggtc aggctgccgg tctgaatggt
      781 aaaggccccg ggatgtgtac cactgtgtcc ataaaacgtg gaattgaccg atgtaaggac
      841 aatggaaccg aacgaagtcg taccaatatt gccggatttg tcaagtgtga tccagccctg
      901 gtcgattgtc aaggtgccaa gaacgcgtcg ggtttgaaac caattgctct ggttccactc
      961 ggcgatggtg ccggctgtag gaccaggcgt attgatggtc aggttgccga agttgcaact
     1021 caccattccg cccagcggga tggaatagtt ataccagcgc ttaatgatca gtactgatcc
     1081 cgggtcgaag ttttctgtac cacgaatgaa aagcgttgca tttaccgcgt cgttggttcc
     1141 tggatcccaa gtaaactggg aggacggccc aagattgaac tgaagtccgg gagaggacag
     1201 ggtgatgtct ccccggttca tcgtgcacgt gccattcacg gtgaaattgc ccgtcatggt
     1261 caaccgcatt ccgctgttaa aggtgagcgt gccgccatcc atgacggtaa ccctgttcac
     1321 ggtgaagttg ttgtccaacg ttaccgtatt ggtggtcagg atctggacgt catccgctgc
     1381 agtcggaacg ccggttggaa gccagatggc ggggttgctc cagttgccgc tcataagact
     1441 ggtcttgatg atggctgacg catcctggtg acaaagcagc gtcatacagc cgagtaggac
     1501 tcggagtatg cggcgtttga aacctgtgcg taataacatc aggacgtgat cggttattga
     1561 ttccggctct cagccccggt ggtcaggggc tttgccatgc gtacgagttg ggtctgatca
     1621 tcaccaagcg gggtattccg gatgaccggt gcagcgatct ccaggtcgtt gacatagtac
     1681 tcttcc
//
LOCUS       DAOTON010000682         1685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_56025_length_1685_cov_3.255828, whole genome shotgun sequence.
ACCESSION   DAOTON010000682 DAOTON010000000
VERSION     DAOTON010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56025_length_1685_cov_3.255828"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1685
                     /locus_tag="P5292_11685"
     CDS             <1..>1685
                     /locus_tag="P5292_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39833.1"
                     /translation="TWTDLSDQLHIKQYYRMGQSATNAGVIYAGAQDNGTDRLANGNW
                     TQVFGADGMDCMVDYTDDDIAYVSYQYGALQRSTDGGNNFNDIAPSSGEWVTPFAIDP
                     VDHNTIYAGYDQVYKSTNRGTNWNTIGPVAIGGGIIALAIAPSNPNYIYTAALDRIYM
                     TSNGGASWNNITTGLPVGSTGITYIAISNQNPQQVWVTLSGYQAGIKVFSSVDGGSTW
                     SNVSGTLPNIPVNCIVYQNGSNDALYIGTDFGVYYRDATLTDWVPYGNDLPNVIVSDM
                     EIYYAGQKLRAATYGRGIWEVDLNPVTALALDAAVIQFIEPAATICSNSVTPVIVVRN
                     NGSDALTDLNINYRLDGGTLQSFAWSGNIASGDTAHVTLPLITVTGGSHSIYAATSDP
                     NGQADQQPANDGYTYNISVNTTVANVPQAEGFEAFSIPADWVLDDAAGMLSYTTSASG
                     FGLSGVSLKVNYFFSSPGATSSIEFPHLDFSAVTNPVYLDFNVAYAVFNATSHDSLKV
                     LLSLDCGETFTAVYAKGDNSLATAPQTMNEFTPDPTQWRAERVDLSAYAGNPNV"
BASE COUNT          386 a          472 c          442 g          385 t
ORIGIN      
        1 acctggacgg acctgagcga tcagttgcac atcaaacaat attaccggat ggggcagtcg
       61 gctaccaacg ccggcgtgat ctatgccgga gcccaggaca atggaaccga ccgtttggca
      121 aacggcaact ggacacaggt attcggagcc gacggaatgg attgcatggt cgattacacg
      181 gatgacgaca ttgcctacgt gtcctaccag tacggtgcct tgcaacggtc aacggacggt
      241 ggcaacaact tcaacgatat cgcgccttcc tccggggagt gggtaactcc gtttgccatc
      301 gatccggtcg accacaatac catctacgcg ggatacgacc aggtttacaa gtcaaccaac
      361 cggggcacca actggaatac cattggcccc gttgccatcg gtggtgggat cattgcattg
      421 gccatagcgc cttccaatcc caattacatc tataccgcag cgctcgaccg tatctacatg
      481 acctcgaacg gcggagcttc ctggaacaac attacgaccg gactaccggt tggaagtacc
      541 ggtatcacct atatcgcgat ctcgaatcag aatccgcaac aggtctgggt gacgctctcg
      601 ggctatcagg caggcatcaa agttttctca tcggtagacg gtgggtcaac ttggtccaat
      661 gtgagcggta cgcttccgaa tattccggtg aactgtatcg tctatcagaa tggctcgaac
      721 gacgcactgt acatcggaac tgattttggt gtgtattacc gcgatgccac tttgacggac
      781 tgggtgccct atggcaacga tctgccgaac gttatcgtca gcgacatgga gatctactat
      841 gccggacaaa agctgcgcgc agccacctac ggccgaggca tttgggaagt tgatttgaat
      901 ccggttaccg cactggcatt ggatgccgcg gtgattcaat tcatcgaacc tgccgcgacg
      961 atctgttcga actctgtgac tccggtcatc gtcgtacgga acaatggtag tgacgcgctt
     1021 acggacctta acatcaatta tcgccttgac ggagggactt tgcaatcatt cgcctggtca
     1081 ggcaatatcg cgagcggcga tactgcacat gttacgctgc ccttgatcac ggtcaccggt
     1141 ggttcgcatt cgatctatgc ggccacctcc gatccgaatg gtcaggccga tcagcagcct
     1201 gcgaatgacg gctataccta caacatctcc gtcaatacga ccgttgccaa cgttccccag
     1261 gcggaaggct tcgaggcgtt ttcaattccc gcggattggg tgctcgatga cgctgccgga
     1321 atgctctcct atacgacaag tgcaagcgga ttcggtctgt ccggtgtctc cctgaaagtg
     1381 aattatttct ttagcagccc gggagcgact tcgtccatcg agtttcctca ccttgatttc
     1441 agtgccgtga ccaatccggt ttaccttgat ttcaatgtcg cctacgcggt attcaacgct
     1501 acttcgcacg attcgctgaa agtgctcctg tcccttgatt gcggagagac ctttacagcg
     1561 gtatatgcaa agggcgacaa cagccttgca acggcgcccc agaccatgaa tgaattcaca
     1621 ccggatccaa cgcaatggcg tgccgaacgg gttgatctga gtgcctacgc cggaaatccg
     1681 aatgt
//
LOCUS       DAOTON010000683         1682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_56152_length_1682_cov_3.071911, whole genome shotgun sequence.
ACCESSION   DAOTON010000683 DAOTON010000000
VERSION     DAOTON010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56152_length_1682_cov_3.071911"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="P5292_11690"
     CDS             <1..641
                     /locus_tag="P5292_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39834.1"
                     /translation="AKTSRIEGLRYGGSAKIIHRKAGDFGKAWGFGIDLGAQYLKGNW
                     QFGLMAKDITSTYNAWSYNTELFEEAFSATGNEIPKNSTEITLPKLIYGAGYRKDITA
                     KISLLAEINADMTFDGQRNTLISSKPISVDPHMRLEVGYNRFIFLRAGLLNIQKVKNF
                     DKTETTIVQPNLGLGVKLGRLALDYALANIGGNETPYSNIFSLRLDVNKRPK"
     gene            687..>1682
                     /locus_tag="P5292_11695"
     CDS             687..>1682
                     /locus_tag="P5292_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39835.1"
                     /translation="MKKTLLLLLLLLALRPMVSKSQTYENDWINYGQQYFKVKVWQNG
                     IYRISGTDLQFSGVPIGSIDPRKVQVFHNGQEEYIHLEGESDGVFNGSDFIEFFGSMN
                     DGSMDTRLYSDSTWQPQPQYSLYTDTAVYFITWSSTTNGRRLTAVNDNNYPAYTPASY
                     FIRESYVMQNYGYNRGMNQNSIDYTESEGWCGVFGNYQGTNFVNNVAVNTANVYSSGP
                     NVEVNTCVGGVNNNPHNMTLVFPGTNFSDTYYMQSIRRYNFSLSPALFTSSSTTFTYN
                     VSTPLSITEYSAFYWLSVRYPHTFDLEAASTFELFVPDDAGQTNTRMDISNFNAGT"
BASE COUNT          444 a          490 c          388 g          360 t
ORIGIN      
        1 atgccaagac ctccaggatc gaagggctcc gttacggcgg tagcgcgaag atcatccacc
       61 gtaaggcagg agatttcgga aaagcatggg gcttcggtat cgacctcggt gcgcaatacc
      121 tgaaaggcaa ttggcagttc ggtttgatgg ccaaagacat cacgagcacc tacaacgcct
      181 ggagttacaa caccgagttg ttcgaagaag ccttcagtgc aaccggaaac gagattccga
      241 agaattccac agagatcacc ctgcccaaat tgatctacgg cgccggttac cggaaagata
      301 ttacggccaa gatcagtttg ctggcggaaa tcaatgccga catgaccttt gacggacagc
      361 ggaacacgct catcagttcc aaacccatca gtgttgatcc gcacatgaga ctggaagtcg
      421 gttacaaccg tttcatcttc ctgcgcgcgg gtctgttgaa tattcagaag gtaaagaatt
      481 tcgataagac cgaaacgacc atcgtacaac ccaacctcgg actcggtgtg aaactgggcc
      541 gacttgcact cgactacgcc ctggccaaca tcggcggtaa cgaaacccca tactcgaaca
      601 tcttctcgct tcgactcgac gtcaacaagc gacccaaata agttccttcc aacggcagcc
      661 cacgaccgag gcaacgaacg atcacaatga aaaaaacttt actccttctt ctgctcctgc
      721 tggcgctccg gccaatggtg tcgaagtccc agacttacga aaacgactgg atcaactacg
      781 gccagcagta tttcaaagtg aaggtctggc aaaacggcat ttaccgtatt tccggaactg
      841 acctgcaatt ctccggcgta ccgatcggca gtatcgatcc gcgcaaggtc caggttttcc
      901 acaatggtca ggaagaatac atccaccttg aaggggaatc ggacggcgtt tttaacggaa
      961 gcgacttcat cgaattcttc ggcagcatga acgacggctc catggatacc cgtctttaca
     1021 gcgactccac ctggcagcct caacctcaat acagcctcta taccgacacg gccgtttact
     1081 tcattacctg gagctccacc accaacgggc ggcgcctgac ggcggtgaac gataacaatt
     1141 atcccgctta cacaccggct tcgtatttca tccgggagag ttacgtcatg cagaactacg
     1201 gctataaccg tggcatgaac caaaactcga tcgattatac cgagagcgaa ggttggtgcg
     1261 gggtattcgg taattaccag ggtaccaact tcgtgaacaa cgttgcggtc aacaccgcca
     1321 atgtatattc cagcgggccc aacgtcgaag tgaacacctg cgtaggcggc gtcaataaca
     1381 acccgcacaa catgaccctg gtgtttcccg gaaccaattt ttccgacacc tactacatgc
     1441 agtcgatacg ccgatacaat ttctcgttat cgcctgcgct ctttaccagt tccagtacca
     1501 ccttcacgta taacgtcagc accccgctct cgattacgga atattcggct ttttactggc
     1561 tgtcggtacg ctacccccac accttcgatc ttgaagctgc ttccacgttc gaactgtttg
     1621 taccggatga tgccggccaa accaataccc gaatggacat cagcaatttc aacgccggca
     1681 cc
//
LOCUS       DAOTON010000684         1680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_56233_length_1680_cov_3.145846, whole genome shotgun sequence.
ACCESSION   DAOTON010000684 DAOTON010000000
VERSION     DAOTON010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56233_length_1680_cov_3.145846"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="P5292_11700"
     CDS             <1..516
                     /locus_tag="P5292_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS39836.1"
                     /translation="VVNLSSGVNLGFNWDFGDGTFSTDPYPSHLYNAVGDYPLCLTVT
                     EPGTGCTSTYCDTLSVDSLGNVFRGMSGFMLNIMSPASLTAVPDLTASPSFAVYPNPF
                     NAACTLQRAASAKGEAVVRVLDLRGAVLFMERFSTGEVRVPADQWSPGTYLIELTEGD
                     GYRSFRKVIKE"
     gene            582..1172
                     /locus_tag="P5292_11705"
     CDS             582..1172
                     /locus_tag="P5292_11705"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRS39837.1"
                     /translation="MKPATELLERCKRDDRRAHYELYRICFPFLVSVCRRYYVNREDT
                     EAAINQIFLKTIINLDRYLKRAENLPFELWLRKIAVNFVVDEFRKNKQYREHFDLREI
                     HEAEADHPTVEPHLEAEQLEAIRSALQQLSDMNRTVFNLHVVDGYKHEEIAAMLNITT
                     GTSKAHLHRAKKRLRELIDPEQVGWQQPNVNKALLP"
     gene            1169..>1680
                     /locus_tag="P5292_11710"
     CDS             1169..>1680
                     /locus_tag="P5292_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39838.1"
                     /translation="MKDEREDFNLDPEASWGKAEQLLDRHFRRKRWGMVLLILLIAGV
                     TTLVTIQLLEPQQSIWQTGRVAQQPVASTTMSSGVSESNTKPSTNALSAEVQVRQPAA
                     DPLTTVVPPSPSTTIAETRPGIALSKRSKSVGAILLTGVEPSKSNVKPVIIEQAADPT
                     INSTAGGYSE"
BASE COUNT          404 a          465 c          448 g          363 t
ORIGIN      
        1 gtggtcaacc tgagcagcgg agtgaatctg ggcttcaact gggacttcgg agacggtaca
       61 ttctctaccg atccctaccc gtcccatctc tataatgccg tcggcgacta ccccctctgt
      121 cttaccgtca ccgagccggg gacaggctgt accagtacct attgcgatac gctttctgtc
      181 gattcacttg gaaacgtatt ccgcggtatg tcgggtttca tgctgaatat catgtcaccg
      241 gccagcctga cggcagtacc tgatctgacc gcttcaccat cgttcgcggt ttacccgaat
      301 ccgtttaatg cggcatgtac gctacaacgt gccgcatcgg cgaaaggtga agcggtcgtg
      361 cgggtactcg atcttcgtgg tgcagtcctc ttcatggagc gctttagcac cggtgaagta
      421 cgagtgccgg cggatcaatg gtcgcccggt acttacctga ttgaactgac cgaaggagat
      481 ggttatcgct ctttccggaa ggtcatcaaa gaatagttcc tgtttcggtt ttttgatgct
      541 tcaggacgga ggagaaatcc tccgtcctgt tgttgcaaac gatgaaacct gccactgaac
      601 tgctggaacg ctgtaagcgg gacgaccgga gagcgcatta tgagctctac cggatctgtt
      661 tccctttcct ggtgtccgtg tgtcggcggt attatgtgaa ccgggaggat actgaagcgg
      721 ccatcaacca gatctttctg aaaacgatca tcaacctgga tcgctacctg aaaagagcgg
      781 agaacctgcc gtttgaactc tggctgcgaa agatcgcggt caacttcgtg gtggacgagt
      841 ttcgaaagaa caagcagtat cgcgagcact tcgacctgcg cgagattcac gaagccgaag
      901 ccgaccaccc gaccgtcgag cctcacctcg aggcagagca actggaggcc attcggagcg
      961 ctttgcagca gttatccgat atgaaccgga cggttttcaa cctgcacgtg gtcgatggtt
     1021 ataaacacga ggagatcgcg gcgatgctca acatcacgac aggaacttcc aaggcgcact
     1081 tgcatcgcgc aaaaaaacgc ttgcgcgaat tgatagaccc tgaacaagtc ggctggcaac
     1141 aaccgaacgt caacaaagca ctcttgccat gaaagacgag agagaagatt tcaacctgga
     1201 tcccgaagca tcctggggta aggccgagca attgctcgac cggcacttcc gcaggaaacg
     1261 gtggggaatg gtgctgctca tcctgctgat cgcaggcgtt acgacgctgg tgaccatcca
     1321 actactggaa ccgcaacaat ccatttggca gaccggccgg gttgcgcaac agccggtggc
     1381 ctcgaccact atgtcttccg gagtatcgga aagcaacacc aaaccctcga ccaatgcctt
     1441 atcggcggaa gttcaagtca ggcaaccggc tgcagaccct ttaacgaccg tggtgccgcc
     1501 ttccccttcg accacgatcg cggaaacgcg tccgggcatc gcactatcaa aacgatccaa
     1561 gtctgttgga gccatcctcc tgacgggtgt tgaaccaagt aaatccaatg taaagcctgt
     1621 aataatcgaa caagcggcag atcctacgat caattcgacg gcaggtggtt attccgaaat
//
LOCUS       DAOTON010000685         1680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_56243_length_1680_cov_2.840000, whole genome shotgun sequence.
ACCESSION   DAOTON010000685 DAOTON010000000
VERSION     DAOTON010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56243_length_1680_cov_2.840000"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="P5292_11715"
     CDS             complement(<1..160)
                     /locus_tag="P5292_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRS39839.1"
                     /translation="MLKKQQRRSNIRYRIRKTVKGTAERPRLSVFRSNKQIYAQLIDD
                     VQGVTLAAV"
     gene            complement(169..723)
                     /gene="rplF"
                     /locus_tag="P5292_11720"
     CDS             complement(169..723)
                     /gene="rplF"
                     /locus_tag="P5292_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRS39840.1"
                     /translation="MSRIGKLPITLPAKVEIKVADGTVQVKGPKGELTQKLNDGITVK
                     IDGTTLTVERATESKEHRAQHGLYRSLINNMVIGVSQGFKKEQELVGVGYKASNQGQL
                     LDLVLGYSHHVIFDIPAEVKVSTKQDKGQNPMIILESADKQLLGAVAAKIRSLREPEP
                     YKGKGVKFVNEVLRRKAGKSAAKK"
     gene            complement(745..1140)
                     /gene="rpsH"
                     /locus_tag="P5292_11725"
     CDS             complement(745..1140)
                     /gene="rpsH"
                     /locus_tag="P5292_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRS39841.1"
                     /translation="MTDPIADFITRIRNAVRANHRVVEIPASNLKKEMTKILKEKGYI
                     LDFKFEDDSKQGLIKIALKYHPVTKAPAIRNLSRISKPGLRKYAGLERMPRVLNGLGI
                     AILSTSKGVMTDKEARTAGVGGEVLCYVY"
     gene            complement(1182..1451)
                     /gene="rpsN"
                     /locus_tag="P5292_11730"
     CDS             complement(1182..1451)
                     /gene="rpsN"
                     /locus_tag="P5292_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRS39842.1"
                     /translation="MAKESMKAREVKRQQMAAKYAAKREALKQSGDYIALDKLPKNAS
                     PVRQHNRCKLTGRPRGYMRIFGVSRVTFRKLALEGKIPGITKASW"
     gene            complement(1485..>1680)
                     /gene="rplE"
                     /locus_tag="P5292_11735"
     CDS             complement(1485..>1680)
                     /gene="rplE"
                     /locus_tag="P5292_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008519.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRS39843.1"
                     /translation="INPKGFDGSGNYTLGVTEQIIFPEIDIDKVNKIQGMDITFVTSA
                     KTDEEAIELLREFGLPFRTN"
BASE COUNT          309 a          456 c          463 g          452 t
ORIGIN      
        1 atacggctgc cagggttacc ccctgcacgt catcgatcaa ctgggcgtag atctgcttgt
       61 tgctgcggaa caccgacagg cggggacgct cggcggtgcc tttaaccgtc ttgcggatgc
      121 ggtagcggat attgctccgg cgttgttgct tcttcaacat ggcttcaatt attttttagc
      181 ggccgactta ccggctttcc gtctgaggac ttcattcaca aatttgacac ccttgccctt
      241 gtaaggttcc ggctcgcgca gcgaacggat cttcgcggca accgcaccca acaactgctt
      301 gtcggcgctc tcgagaatga tcatggggtt ctggccctta tcctgcttcg tcgagacttt
      361 cacctcagcc gggatgtcga aaatcacgtg gtgcgaataa ccgaggacga gatcgagcaa
      421 ttgaccctgg ttgctggcct tgtagccgac acccacgagt tcctgctctt tcttgaagcc
      481 ctgggataca ccgatcacca tgttgttgat cagcgagcga tacaagccgt gctgggcacg
      541 atgctctttc gattcggttg cacgctcgac ggtcaacgtg gttccgtcga tcttgacggt
      601 gataccgtcg ttcagcttct gcgtcagctc gcccttcgga cctttcactt gaacggttcc
      661 gtcggcgacc ttgatctcca ccttagcagg cagggtgatc ggaagtttac ctatgcggga
      721 cattgtagtt ggatgcttgt tgaattaata aacgtagcag agtacttctc cgcctacacc
      781 ggccgtgcgg gcctctttat cggtcatcac gcccttcgag gtcgagagga tcgcaatgcc
      841 cagcccgttg agtacgcgcg gcatgcgctc cagacccgcg tatttgcgga gacccggttt
      901 gctgatgcgc gagaggttcc ggatggccgg agccttggtc acgggatggt acttcagggc
      961 gattttaata aggccctgct tggaatcgtc ttcgaacttg aagtccagga tgtacccctt
     1021 ctccttcagg atcttggtca tctccttctt gagattcgaa gccgggatct ccacgacacg
     1081 gtggttggcc cgtacggcat tgcgaatccg ggtgatgaag tctgcgatcg gatcagtcat
     1141 cgtattaagg gtgttgtagt ccgttagtct attgttatga attaccagct ggctttggtt
     1201 atgccaggaa tcttgccttc caacgcaagt ttgcggaagg tcacgcggga aacgccgaag
     1261 atgcgcatgt atccgcgggg acgaccggtc agcttgcagc gattgtgctg acgtaccgga
     1321 gaagcgttct tcgggagttt gtccagagcg atgtaatcgc cagactgctt cagcgcctca
     1381 cgcttggctg catacttcgc ggccatttgc tgacgcttta cttcccgtgc tttcattgat
     1441 tcttttgcca tgtcgatatc cgtgttggat gttattgtct gcgattaatt ggtccggaac
     1501 ggcagtccga attcacggag cagctcgatc gcctcttcgt ccgtctttgc cgacgtaacg
     1561 aatgtgatgt ccataccctg gatcttgttc accttgtcga tgtcgatctc cgggaaaatg
     1621 atctgttcgg tcacacccag cgtatagttg ccgctgccgt cgaatccttt cgggttgatg
//
LOCUS       DAOTON010000686         1679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_56309_length_1679_cov_2.800493, whole genome shotgun sequence.
ACCESSION   DAOTON010000686 DAOTON010000000
VERSION     DAOTON010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56309_length_1679_cov_2.800493"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..>1679
                     /locus_tag="P5292_11740"
     CDS             107..>1679
                     /locus_tag="P5292_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUB domain-containing protein"
                     /protein_id="HRS39844.1"
                     /translation="MRILLRSTLVLLLFFSGKAYAQNYNMGNSSISTCSGNFYDSGGN
                     GAGAGANYNNNENYTMTFCSSTPGQCVRLTFTAFNIESGFDYLRIYNGSTTGAPLLGT
                     YTGTTSPGTVTATSGCLTLQFTSDGSINYAGWAATVSCVTCPGGPCGSTCSGGPAPAN
                     DACSGAQNLGLLPVPASCPSGVGTPLSLNTTNLCATAEMPYTSMLGCQPTGNMASPAS
                     DVWYQFSITGPTLNINLTGLQTPEVGLYSGSNCNNLVPRGCAIGASGTLNTSFGGLAP
                     GTYWLQVSGGNLNDQCNFNLQLQNNFDCAGCVMVSNLTVNPPPVNGTYQAGQLVNFCY
                     TISDYNQTSVNWLHAVIPSFSAGWDLSTLTTTMPANCSGAGAWTWNNGLVTSSATGLV
                     VGPGFFYESALGSPGGVMDGNPGNNFGDNNALNTCDWTFCFSIRTLPPGQCIPGLPLN
                     IAIDTYGDGETGSWTSFACSGDPITQFFAQLACCTPPVVNDFQPPCPGSTGSAVATGQ
                     GVAPWNYIWRNGGGTV"
BASE COUNT          371 a          501 c          406 g          401 t
ORIGIN      
        1 tgagccgccc aagtatcggg cactcaactt gctggccatt ttacctcgaa gacgattccg
       61 atcttcttcg aaaccttaac tcttagcacg taaccacaca cgacgcatga ggatactttt
      121 acgctccaca ctcgtacttc tgctcttctt ttccggtaaa gcctacgccc agaactataa
      181 catgggcaat tcttcgattt ccacctgtag tggaaatttc tatgacagcg gtggcaatgg
      241 cgccggggca ggcgcgaatt acaataacaa cgagaactac acgatgactt tttgctcgag
      301 cacacccggg caatgtgtgc gactgacatt taccgcgttc aacatcgagt caggatttga
      361 ttacctgaga atatacaatg gctcaaccac cggcgctccc ctactcggca cctataccgg
      421 aacaaccagc cctggaaccg taactgcaac cagcggttgt ttaacacttc agtttacctc
      481 ggatggttcg atcaattatg caggatgggc cgcaaccgtt tcgtgcgtga cctgccccgg
      541 cggaccttgt ggttcgacct gcagtggcgg acctgcccct gccaacgacg catgcagtgg
      601 agcgcagaac cttggcctac tgccggttcc ggcttcgtgt cccagcggag tcggcacgcc
      661 actttccctg aataccacca acctgtgcgc tacggcggaa atgccttaca cctccatgct
      721 tggctgtcag cctaccggca atatggccag tcctgcctcc gatgtctggt atcaattcag
      781 catcacagga ccgacgctga acatcaacct tacggggcta caaacaccgg aggttggttt
      841 gtattccggc agcaactgca acaacctcgt accgcgtggt tgcgcgattg gcgcgagcgg
      901 aacgttgaat acatctttcg gcggacttgc gcccggtacc tactggttgc aagtatccgg
      961 tggaaacctc aacgatcagt gcaatttcaa tcttcaacta cagaataatt tcgactgcgc
     1021 gggttgcgtc atggtcagca acctgaccgt caacccacca ccggtaaatg gaacttatca
     1081 ggctggacaa ctcgtcaatt tctgctacac gatatccgac tataatcaga cttccgtcaa
     1141 ctggctgcac gccgttattc ctagcttttc agccggctgg gacctcagta ccctgacgac
     1201 taccatgccg gccaactgtt ccggtgccgg tgcctggacc tggaacaacg gccttgtgac
     1261 cagttccgct acaggacttg tcgtaggacc ggggttcttc tacgaatctg cactcggaag
     1321 tcctggcggt gtcatggatg gtaatccggg gaacaacttt ggcgataaca atgcactgaa
     1381 tacctgcgac tggacctttt gcttttccat tcggacctta ccaccaggac aatgtatacc
     1441 cggacttcca ctcaacattg ccatcgacac ttacggtgat ggtgaaaccg gctcctggac
     1501 ttcctttgct tgcagcggcg atccgatcac gcagttcttt gcacagcttg cgtgttgtac
     1561 gcctcccgtt gtcaatgatt ttcaacctcc ttgtcccggc tcgacgggtt cagccgtcgc
     1621 taccggccag ggtgtcgcac catggaatta tatctggcga aatggcggcg gcaccgtta
//
LOCUS       DAOTON010000687         1679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_56312_length_1679_cov_2.676108, whole genome shotgun sequence.
ACCESSION   DAOTON010000687 DAOTON010000000
VERSION     DAOTON010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56312_length_1679_cov_2.676108"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="P5292_11745"
     CDS             complement(<1..527)
                     /locus_tag="P5292_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS39845.1"
                     /translation="MGTLSALLVDDEQKNRDSLRKLLELYCPDVQVVGEADSVEGALE
                     ALERLEPELVFLDVEMPGGTGFDFLRRAGERNFRVIFVTAHSHYAIKAIRFSAIDYLL
                     KPVDTDDLVEAVRKAMEERSQSSAPQHQHLLESLGQAGIRKLAIPIRDGIAFLAPEEI
                     VRLEADGTYTHVYTL"
     gene            complement(644..>1679)
                     /locus_tag="P5292_11750"
     CDS             complement(644..>1679)
                     /locus_tag="P5292_11750"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRS39846.1"
                     /translation="SVNERSVPAERRISLAHDENTIQVRFNAVSFRSGGQLRYRYQLT
                     NGTDTVSGTTTSREVQFLALRPGAYRFSVTAINASGVVSEQPAVAEFHIHPAWWQTIV
                     FRLLLVLVLVGLIYLLYRRRLKLIGARYAMERKQASLQLTAMRAQMNPHFIFNVMNSI
                     RSYMLNKDTASAEKYLTSFSRLVRYTLDHSDEQEVSLEEELKALSSYVELEQQRFTSP
                     FQFRMDLDPAVDVQEFTLPSLLLQPFVENAIKHGIARRGGDGKIVIRLEAAGDGLRIL
                     IEDNGVGRRAAEAHRSSSDPAHVSHGTGIAFARIEAFNKAFNRQIRATYSELNPGDGE
                     FPGTRVEIGF"
BASE COUNT          387 a          521 c          443 g          328 t
ORIGIN      
        1 tgcaaggtat agacatgtgt gtaggtaccg tccgcttcca accgtacgat ctcctccggg
       61 gcaagaaacg cgatgccgtc acggatcggg atcgccagtt tgcggatacc ggcctgcccc
      121 agactctcca acaagtgctg gtgctgcggt gcactggatt gcgaacgctc ttccatggcc
      181 ttacgcaccg cctcgaccag gtcgtcggtg tcgaccggct tcaacaagta atcgatcgcc
      241 gaaaaacgga tcgccttgat cgcataatgc gaatgcgcgg taacgaagat gacccggaaa
      301 ttccgctccc cggctcgtcg cagaaaatca aaaccggtgc cacccggcat ttccacatcc
      361 aaaaaaacca gttccggctc cagacgctcc aatgcttcca aggccccctc caccgagtcc
      421 gcctcgccga ctacctgtac gtccggacaa taaagctcca acagcttccg caacgaatcg
      481 cggttcttct gttcgtcgtc aaccagcagt gccgaaagag tgcccatgta ctagcaaggt
      541 aaggaatcgg cgggagaaag cacgcgagat gagggacaag gggcgtggga cgtgggacga
      601 aacaacacgc ccacctgccg aagctttagc gaaggcaggg aactcaaaac ccaatctcca
      661 cccgcgtccc cggaaactcc ccatcccccg gattcaactc cgagtaggtc gcccggattt
      721 gtcggttaaa ggctttgttg aaggcctcga tacgcgcgaa ggcgataccg gttccgtggg
      781 agacatgcgc cggatcactg ctgctacgat gcgcttccgc ggcccggcgt ccgaccccgt
      841 tgtcttcgat caggatgcgc aatccgtcgc cagccgcttc cagccggatc acgatcttcc
      901 cgtctccacc acgacgcgca ataccatgct tgatggcatt ctcgacaaaa ggttgcaaga
      961 gcagggaggg tagcgtaaac tcctgcacat ccaccgccgg atccaagtcc atcctaaact
     1021 gaaaaggcga agtaaaccgc tgctgctcca gctccacata agagctgagt gccttcaact
     1081 cttcctccag gcttacctcc tgctcgtccg aatgatcgag ggtataacgc accaggcgcg
     1141 agaacgaggt gaggtacttc tccgcggaag ccgtgtcttt gttcagcata taactccgga
     1201 tggagttcat cacattgaag atgaaatgcg gattcatctg cgcccgcatg gcggtcagtt
     1261 gcaaactggc ttgcttccgc tccatcgcgt aacgcgctcc gatcagcttc aatctgcgcc
     1321 gatacaggag gtaaatcagg cctaccagga ccaataccag taaaagccgg aatacgatcg
     1381 tctgccacca ggccggatga atgtgaaact ccgctaccgc cggttgttca ctgacgactc
     1441 ccgatgcatt gatggccgtc accgaaaacc ggtaagcgcc cggtcgtaaa gccaggaact
     1501 gcacttctcg tgatgtcgtg gttccgctga cggtatcggt tccgttcgtc aactgatacc
     1561 ggtaacgaag ttgtccgcca ctacggaagg agacggcatt gaaccggacc tggatggtat
     1621 tctcgtcgtg tgcgagactg atccggcgct ccgcgggaac gctgcgctca ttcacactc
//
LOCUS       DAOTON010000688         1678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_56347_length_1678_cov_3.609365, whole genome shotgun sequence.
ACCESSION   DAOTON010000688 DAOTON010000000
VERSION     DAOTON010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56347_length_1678_cov_3.609365"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..200
                     /locus_tag="P5292_11755"
     CDS             18..200
                     /locus_tag="P5292_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39847.1"
                     /translation="MNSTSFLSLPGGVDVEFTGQVITYPDGTLLTRNGLKVDRPVRPT
                     AAAISAGKDLLLESVR"
     gene            complement(205..762)
                     /locus_tag="P5292_11760"
     CDS             complement(205..762)
                     /locus_tag="P5292_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HRS39848.1"
                     /translation="MNRINVIDLDGTLLPYDSVSRWIRHFKRLEGWFSPLRRWSLLYR
                     FGWMDRSTFIQKCVDHLRQRDDYTDFNREFAGKLVQDISKTTLQQIEQHTDALTVNIL
                     CSASPDDYVSLVAGHLKWECRASGYRADGRWQLLYGTEKLKRIREDFSPDTYRYHFAI
                     ADDASDLELLGAFEHSVLIRKGDPR"
     gene            complement(759..>1678)
                     /locus_tag="P5292_11765"
     CDS             complement(759..>1678)
                     /locus_tag="P5292_11765"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRS39849.1"
                     /translation="DPGQNPNAFIRDLQSICEKERIDRVFPVNSREYRLLLPHRASFN
                     GRLDHLPTLEQFRRLDNKVDFSALAATLQLPVPRHYRLEDDPVTFPLVFKPAESASAK
                     GVRYLHDRSELTRLRNTYSESDYVLQEYIQGFGAGYSVMAINGKIIAGCGHRRRAEWP
                     VSGGSSMVRSYYKHAGMEAVAEKVMRHLQWTGVAMFEFKIESSGNARLIEINPRWWGS
                     LYQSIATGVNLPVISLFPDQSGTTTLREQTITYNSPWHLRSFAHYVLKRGDWKPIVYF
                     LRHYLQAKADVSITRDPLGYLSGLLRFLS"
BASE COUNT          389 a          439 c          472 g          378 t
ORIGIN      
        1 aggtgatcac ttgaccggtg aattcgacga gtttcctctc gctgcctggt ggcgtggacg
       61 tggaattcac cggtcaagtg atcacctacc cggacggcac cctgcttacg cgtaatggat
      121 tgaaagtgga tcgtccggtt cggcccacgg cggcagctat cagtgccggg aaggacctgc
      181 tgctggaatc ggtgcgttga gtgctcagcg cggatcgcct tttcggatca atacggaatg
      241 ctcgaatgcg cccagtagtt cgaggtcgct cgcgtcatcc gctatcgcaa aatgataacg
      301 atacgtatcc ggtgaaaagt cttcccgtat ccgtttgagc ttttccgtac catacaacaa
      361 ttgccagcga ccatcggccc ggtacccgga agcgcggcat tcccatttca aatgaccggc
      421 gaccaggctg acataatcat ccggcgatgc cgagcagaga atgttcacgg ttagcgcgtc
      481 cgtatgttgt tcaatctgtt gaagcgttgt tttggaaatg tcttgaacga gtttcccggc
      541 aaactcccga ttgaagtcgg tatagtcgtc gcgttgccgt aggtgatcaa cgcatttttg
      601 aatgaaagtg gatcgatcca tccagccgaa acggtaaagc aggctccagc ggcgcagggg
      661 agagaaccag ccttccagtc gtttgaagtg ccggatccaa cgactcaccg aatcgtatgg
      721 cagcagggtg ccgtcgaggt caatgacgtt gatgcggttc atgacagaaa acgtaagagt
      781 ccggacaggt aacctagcgg atcacgggta atcgatacat ccgcctttgc ctgaagataa
      841 tgccggagga aatacacgat gggcttccaa tccccacgct tcagtacata gtgtgcaaag
      901 ctccttaaat gccagggtga gttgtaggtg atggtttgct cacgtaaggt tgtcgtaccc
      961 gactggtccg ggaaaagtga aatgaccggc agattgacgc cggttgcgat actctgatag
     1021 agtgatcccc accagcgggg attgatttca atcaggcgag cgtttccgga tgattcgatc
     1081 ttgaattcaa acatggccac accggtccat tgcagatgcc gcataacttt ttcagcaacg
     1141 gcttccatgc ctgcatgctt gtaataggag cgtaccatgc tggagccacc gctgaccggc
     1201 cattcggcgc gacgacgatg gccgcaaccg gcgatgatct ttccgttaat cgccatcacc
     1261 gaatatcccg cgccgaagcc ctggatatat tcctgcaaca catagtcact ttcggaatac
     1321 gtattgcgca gccttgtcaa ttcagagcgg tcatgtaaat accggacccc ttttgcggaa
     1381 gcggactccg ccggtttgaa caccaggggg aatgtgaccg gatcgtcttc cagccgatag
     1441 tgccggggca cgggcaactg cagcgtagcg gccagtgcgg aaaaatcgac tttgttgtcg
     1501 agcctacgga attgctctaa ggttggcaga tggtcgagtc tgccgttgaa agaagcgcgg
     1561 tgcggaagca acaagcgata ctcccggcta ttgaccggga aaacgcgatc gatccgttcc
     1621 ttttcacaaa tcgactgaag atcccggatg aacgcgttag ggttctgccc gggatccg
//
LOCUS       DAOTON010000689         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_56458_length_1676_cov_2.819864, whole genome shotgun sequence.
ACCESSION   DAOTON010000689 DAOTON010000000
VERSION     DAOTON010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56458_length_1676_cov_2.819864"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="P5292_11770"
     CDS             complement(<1..366)
                     /locus_tag="P5292_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRS39850.1"
                     /translation="MKELATLFFRLGFTAFGGPAVHIAMMQEEVVTKRKWMTHDHFLD
                     LVGATNLIPGPNSTELAIHIGHERAGWRGLLLAGVCFIFPAVVLTACLAWLYKRYGTL
                     PDLQPYLYGIKPAIIAVVLS"
     gene            complement(373..801)
                     /locus_tag="P5292_11775"
     CDS             complement(373..801)
                     /locus_tag="P5292_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRS39851.1"
                     /translation="MVNRWLLTSTVPTLIPRIIVGLVFLSEGIQKFTFADSVGAGRFA
                     KIGFENAEFWAGFTGCFEIVCGLLVLFGLFTRLAALPLLVVMIVAFITTKYPTLVKQG
                     FFPMAHEYRTDFAMTLLLLMLIRYGGGRPSLDHRWFGDKQ"
     gene            complement(791..1300)
                     /locus_tag="P5292_11780"
     CDS             complement(791..1300)
                     /locus_tag="P5292_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39852.1"
                     /translation="MMQEKLKRYYSMILLLALSGCGSGNVEQPNTQIRLDSLEARLSE
                     AYKPGFGEMMGSIQVHHNKLWFAGVFFFKQKTAYEIHEIQETLEQITKFQQEREESRM
                     TGSLLPRLDSIQLAVSEGKVDRFREQYALLTDACNDCHRATDFGFNVVKIPDEPPFTN
                     QEFKLKDGQ"
     gene            complement(1316..>1676)
                     /locus_tag="P5292_11785"
     CDS             complement(1316..>1676)
                     /locus_tag="P5292_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39853.1"
                     /translation="TGYLYAVRFLDAQTGFVLGDMGAIFKSSGSTAIHIPPRATDMKV
                     FPNPLANGTVLHWETLSACTGLMEIVDMQGACVHRKTISGVQGSVDLTDVPSGQYVIQ
                     LMENGKLLHREILLINR"
BASE COUNT          437 a          475 c          380 g          384 t
ORIGIN      
        1 cgacaacaca accgcgataa tggccggttt gatcccgtat agatacggct gtaaatcggg
       61 aagcgtacca tagcgtttat acaaccaggc caagcaggct gtcaggacaa cagcgggaaa
      121 aatgaaacaa acgcccgcga gcagcaagcc tctccaaccg gcacgttcat ggccgatgtg
      181 tatggccaac tcggtgctat tcgggcccgg tatcaggttg gtcgcgccca cgagatcgag
      241 gaaatgatca tgcgtcatcc acttcctctt cgtaaccacc tcctcttgca tcatggcgat
      301 gtgtacagcc ggtcctccga atgctgtaaa tcccaaccgg aaaaacaaag tggcaagttc
      361 tttcatcccg ggctattgct tgtcgccaaa ccaacgatga tccagcgacg gccgcccacc
      421 gccatagcgg atcaacatca ggagcagaag agtcatcgcg aaatccgtcc tgtattcgtg
      481 cgccatcgga aagaagcctt gtttgacgag ggtaggatac ttcgtcgtaa tgaacgcaac
      541 gatcatcacg accaacaatg gcagggcagc caatcgggta aaaagtccga acagcaccaa
      601 cagtccgcaa acaatctcga aacacccggt aaaaccagcc cagaattcag cgttctcaaa
      661 gccgattttc gcgaaccttc ccgctccaac actgtctgca aatgtgaatt tctgaatccc
      721 ttccgagagg aataccagtc cgacaatgat tcgcggaatc aatgtcggca cggtcgatgt
      781 aagcaaccac ctattgacca tccttcaact tgaattcctg gtttgtgaat ggcggctcat
      841 cggggatctt caccacattg aatccgaaat ccgtggcccg atgacaatcg ttgcaggcgt
      901 cggtcagcaa tgcgtattgc tcgcggaacc gatcgacctt tccttccgat acagctaatt
      961 gaatactgtc caatcgcggg agcagcgatc cggtcatccg actctcctca cgttcctgtt
     1021 gaaacttcgt gatctgttcc agtgtttcct gaatctcatg gatctcgtat gccgtcttct
     1081 gcttgaaaaa aaaaacacct gcaaaccaga gtttgttgtg atggacctga atgcttccca
     1141 tcatttcacc gaatcccggc ttgtatgctt cggacagcct ggcttccaga ctatccagcc
     1201 tgatctgcgt gtttggctgc tctacgttcc ccgatccgca acctgacaag gccagcagga
     1261 ggatcattga ataataccgc ttcagctttt cttgcatcat gcaattccca atccgttaac
     1321 gattaattaa caagatttcc cggtgaagca atttaccatt ttccattagt tgaatgacat
     1381 actgccccga aggaacatcc gtcaaatcga cgcttccctg aactccactg atcgtcttac
     1441 gatgcacaca cgcaccttgc atatccacga tttccatcaa acctgtgcag gcactgaggg
     1501 tttcccaatg aagtacggtc ccgttcgcca atgggtttgg gaaaactttc atatcggtcg
     1561 cccggggagg gatgtgaata gcagtactcc ctgaggattt aaagattgca cccatatcac
     1621 cgaggacgaa accggtctgc gcatcgagaa agcgaaccgc atacaggtag cccgta
//
LOCUS       DAOTON010000690         1674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_56591_length_1674_cov_2.018530, whole genome shotgun sequence.
ACCESSION   DAOTON010000690 DAOTON010000000
VERSION     DAOTON010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56591_length_1674_cov_2.018530"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="P5292_11790"
     CDS             <1..648
                     /locus_tag="P5292_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRS39854.1"
                     /translation="LGTPFFTLIQTISTHEPASPPGNTKYRSSFQTPFEQAYDYTDWC
                     VGRFFETAATRPWYDSTVFVLVGDHGRPFVEDYPAPLAFNHVLLLIRTPGMGDTLRAP
                     INQPVMQIDLFPTIMGILQQPYLNTTPGADVFREPRPYAYFSQDHQLAVVGKDQLYIE
                     NKYGSRFLYALDDRSKTNRLEQSLSLADSMAAYAHDALQMTDWVLEHRLAGYPGK"
     gene            complement(697..>1674)
                     /locus_tag="P5292_11795"
     CDS             complement(697..>1674)
                     /locus_tag="P5292_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39855.1"
                     /translation="NIFDSGHTLYVDGTHLYVASVSQTGGSYSSMNVYDVSSPATPVL
                     LRRLDQDYPSISQVHDMFVINDTIFASCGYQGLYIFTYDESTNRFTQRGSLTNYTPGG
                     SYNHSSFISNDRTTLYMMDEVPDGLPCKVVDVSDLSNPFVVDTFYSNAGCTPHNPYVK
                     GNLLVNANYQDGVWIYDISNPQVPVFAGYFDTHPQNSPGTYPNPPYAGCWAAYTDLPS
                     GTLLASDMQLGLFCLDIGTITGLSTRPSLNPVRLFPNPAQDRLTIALSESSTAGTYLL
                     TDLQGRELMNGELNAATTTLDISRLNPGFYLVKTQLGGQVRSTRIQITR"
BASE COUNT          366 a          426 c          474 g          408 t
ORIGIN      
        1 cttggaactc cgtttttcac cctgatccaa acgatcagta cccatgaacc ggcttctccg
       61 cccgggaata cgaagtaccg aagcagtttc cagactccgt ttgagcaggc ttacgattat
      121 accgattggt gcgtcggacg cttctttgaa acggccgcta cgcgcccgtg gtacgattcc
      181 acggttttcg tactcgtcgg agatcatggc cgaccgtttg tggaagacta tccggctccc
      241 ctggccttca atcatgtgtt gttgctgatc cgtaccccgg gaatgggtga taccctccgc
      301 gcaccgatta atcagccggt gatgcaaatc gatctgttcc ccaccatcat gggcatcctg
      361 cagcagccct atctgaatac aacacccggg gccgatgtgt ttcgcgaacc acgaccctac
      421 gcatatttct cacaggatca tcaattggct gtggtcggta aagatcagtt gtacatcgag
      481 aataaatatg gtagccgctt tttgtatgcg ttggacgatc ggagtaaaac caaccggttg
      541 gagcagtcgc tttccctggc cgacagtatg gctgcctatg cacacgacgc gctccagatg
      601 accgattggg tgctggagca tcgtctcgca ggctatcctg gaaaataata aagcgaccat
      661 ggtggccgct ttcttctgtc aatgtaattg tatcgcttaa cgggtgattt gtattcgcgt
      721 gctgcgaacc tgcccgccaa gttgggtttt tacgagataa aaacccgggt tcagccgact
      781 gatatcgagc gtagtcgtag ccgcattcaa ttcgccattc atcaattccc ttccctgaag
      841 atcggtcagc aggtacgttc ctgcggtgct tgattcggat aaggcgatgg taagtcgatc
      901 ctgcgccggg ttggggaaaa gtcgtacggg atttagcgaa ggccgcgtac tcagcccggt
      961 gatggtccca atgtcgagac agaataatcc aagttgcatg tcgctcgcca gcagcgttcc
     1021 gctggggaga tcggtgtaag cggcccagca gcctgcatag ggcgggttgg gatacgtgcc
     1081 cggactgttt tgcgggtgcg tgtcgaaata tccggcgaaa acaggcactt gcgggttgct
     1141 gatgtcgtag atccaaacgc cgtcctggta attggcattg accagtaaat tgcctttcac
     1201 atacgggttg tgcggtgtac aacccgcatt cgaatagaat gtgtccacga cgaacggatt
     1261 actcagatcg gaaacgtcga ccactttgca gggcaatccg tcgggtactt catccatcat
     1321 gtacagggtg gtccggtcgt tggaaatgaa gctgctgtgg ttgtagcttc ctcccggggt
     1381 atagttggtg aggcttccgc gttgagtgaa acggttcgtg gattcgtcgt aagtgaaaat
     1441 gtacagtccc tggtaaccgc aggaggcgaa gatggtgtcg ttgatgacaa acatgtcatg
     1501 cacctggctg atggagggat agtcctggtc gagtcggcgc aagagcacgg gagttgccgg
     1561 agacgaaacg tcgtacacgt tcatgctgga ataactcccg ccggtctgac tgaccgatgc
     1621 cacatacaaa tgtgtgccgt cgacatacag cgtgtggccg ctgtcgaaga tgtt
//
LOCUS       DAOTON010000691         1671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_56721_length_1671_cov_2.572401, whole genome shotgun sequence.
ACCESSION   DAOTON010000691 DAOTON010000000
VERSION     DAOTON010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56721_length_1671_cov_2.572401"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="P5292_11800"
     CDS             <1..254
                     /locus_tag="P5292_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39856.1"
                     /translation="DMGYAIGALLTGLIADQFGINASITVIGILTLISALVIEYRMRC
                     RTEAPRLTDWLLNRKKKSCRKAPANNYTSSETLAGFSLE"
     gene            complement(364..448)
                     /locus_tag="P5292_11805"
     tRNA            complement(364..448)
                     /locus_tag="P5292_11805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(412..414),aa:Leu,seq:taa)
     gene            complement(660..735)
                     /locus_tag="P5292_11810"
     tRNA            complement(660..735)
                     /locus_tag="P5292_11810"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(700..702),aa:Gly,seq:gcc)
     gene            complement(853..>1671)
                     /locus_tag="P5292_11815"
     CDS             complement(853..>1671)
                     /locus_tag="P5292_11815"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HRS39857.1"
                     /translation="IDALVWNAPGFSNGTTNYGSADCIGAHAWTNSGIPDTGRSLLNF
                     DIDLIPTNAAVVSADLSLYSNPGTAYAGGQHSSFSWPNAGVIRRITSPWREDSVIWFT
                     QPTFTFVNEVVLPPSIGPYDDYLNMDVTQLVQDMIQNSQSSYGFEFALLDEHYYSSLV
                     FTSSDAADPSRYPKLEICYSITNGLQPMNSNATEPRVFPNPVGDRAQLSYDPTVYSDA
                     VLQLLTLQGQVIRNLGITTQTGYAELDLTDLSSGVYMLRIQCAGAVKSLPVIKR"
BASE COUNT          437 a          439 c          399 g          396 t
ORIGIN      
        1 gcgacatggg ttacgcgatc ggagccttac tgaccggtct gatcgctgat caattcggca
       61 tcaacgcttc catcacggtg atcggaattc tcaccctgat ttcagccctc gtgatcgaat
      121 accgcatgcg ttgtcgcacc gaagcacccc gcctcaccga ctggcttctg aataggaaaa
      181 agaaatcctg cagaaaagct ccggcaaaca attacacctc gtcggaaaca ctggccgggt
      241 tcagccttga atagactgga aaattggaat gtcagaaact ggtaactagt aattactaat
      301 tagtaactga aacctgaatt cgaaccacta attactaatt tctagttacc aattccccct
      361 ccgtacccga ggcgggaatc gaacccgcac ggccgcaatg gccaagggat tttaagtccc
      421 tcgtgtctac cagtttcacc acccgggcat cttacgtttg aacgttaaaa cgttgtaacg
      481 ttgtaacgtt gaacgttgaa cgttgcaggt tgaaagttaa caccccaaat ccatccaacc
      541 caataaccaa tcactaatca cgaatcacta gtttctagat tttagttacc agttaccagt
      601 ttctacttcc cagttccccc cttcccaaaa aaaaatcccc cgttaccgga ggactttttt
      661 ggagcggaag accgggctcg aaccggccac cctcaccttg gcaaggtgat gctctaccaa
      721 atgagctact tccgcttaag ttcgcttccc gcccgtggcg ttgaagcgag gacaaaagta
      781 ctgctttttt tattagaaat caacgtctga ggagaaaaaa acgccttttt tcgcaaaaaa
      841 actgactgat tttcaacgct taatcacagg aagacttttc accgctcctg cgcactggat
      901 acgtaacata taaacgcccg aagacaggtc ggtcaggtca agttcagcat atccggtttg
      961 tgtggttata ccgaggtttc ggattacctg cccctgcagg gtcagcagtt ggagtacggc
     1021 gtcggaatag acagtagggt cataactaag ttgagcacga tcaccgaccg gattcgggaa
     1081 taccctcggc tccgttgcgt tggaattcat cggctggagt ccgttcgtta tggaatagca
     1141 aatctccagc ttgggatatc gggatgggtc tgcagcatcg ctcgacgtga acacgaggct
     1201 actgtaatag tgctcgtcca acaaggcaaa ttcaaatccg tagctggact gcgaattttg
     1261 tatcatatcc tgcacaagct gtgttacgtc catgttcagg taatcgtcgt atgggccgat
     1321 cgaaggggga agcaccactt cgttgacaaa ggtaaacgtc ggttgtgtga accagatcac
     1381 cgaatcctcc cgccagggag aagtgatccg cctgatcacg cccgcattcg gccaggaaaa
     1441 agaggaatgt tgtccgcccg cgtaggcggt tcccggattg ctgtagagcg acaagtcagc
     1501 agaaacaaca gctgcattcg tcggtatcag atcgatatcg aagttcagta aagacctgcc
     1561 ggtgtccggt atgccggaat tcgtccaggc atgcgcgccg atgcaatccg cgcttccgta
     1621 attggtcgtg ccattcgaaa aacccggtgc gttccagaca agggcgtcga t
//
LOCUS       DAOTON010000692         1670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_56769_length_1670_cov_2.830960, whole genome shotgun sequence.
ACCESSION   DAOTON010000692 DAOTON010000000
VERSION     DAOTON010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56769_length_1670_cov_2.830960"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="P5292_11820"
     CDS             complement(<1..1427)
                     /locus_tag="P5292_11820"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS39858.1"
                     /translation="MNAQCASTVSSFPYFESFEAGNGGWSSSGSNDDWALGTPGKPVI
                     RTAGDGLNSWITGGLTLSFYNFGERSWVESPCFDFSGLQFPEVSLMVFWETEYQYDGA
                     NLQYSIDGGSSWVNVGNANESGTCLSENWFNRSGINNLSGFVNPGSGWSGNVQPTSGS
                     CRGGNGSGDWVTARHCVRELAGQPEVRFRVTFGSGTTCNDFDGFAFDAFQIRESSSAA
                     TAGVLFSCVNETTVQFSDADPSCNQSWSWDFGDGTSGSSSETVSGTSHTFLTPGTYTV
                     TLETENACGASAVGTQEINVLRATSVTQAVSCAGGNDGSVSLEVLPSGLAHTTVWQLP
                     VPVVGDSLLGLEEGVYDAIVSVSNGCSLEVSTGIETRPDGYVRPELGPDTFVCPDRPL
                     QLGGGVFDQYLWSTGDDGPFVLISDSGVIALQVTNARGCTGTDTLHVRLDCLGEPLLP
                     NAFTPNQDGINDAWSPVGPLERVIG"
BASE COUNT          437 a          468 c          416 g          349 t
ORIGIN      
        1 cagccgataa cacgctccaa tggtcctacg ggcgaccagg catcgttaat accatcctga
       61 ttgggtgtaa aggcattcgg caataagggt tcgcccagac aatccagtcg cacatgtaac
      121 gtatcggtac cggtacaacc gcgtgcattg gtcacttgca aagcaattac gcctgaatct
      181 gaaatgagca caaacggtcc atcatccccc gtcgaccaca aatattgatc aaacactccc
      241 ccgcccagtt gcaggggccg gtccgggcag acaaacgtgt cagggcccaa ctcgggacga
      301 acgtacccgt cgggacgagt ttcaatgccg gtggagactt ccagcgagca accgttgctg
      361 accgaaacga ttgcgtcgta tactccttcc tcaagtccca aaaggctatc tccgacaacc
      421 ggaaccggaa gttgccagac agtcgtatgt gcaagaccgg aaggcaggac ttcgagggag
      481 acgctcccat cgttaccacc ggcacagcta acggcttgtg ttaccgaggt agcgcgcagc
      541 acgtttattt cctgcgttcc gactgccgaa gcaccgcatg cattttcggt ttccagggtc
      601 accgtatagg tgcctggagt taaaaatgta tgcgaagttc cggagacagt ctccgaagaa
      661 gaacccgaag ttccgtctcc gaaatcccag gaccaggatt gattacagga aggatcggca
      721 tccgagaatt gcaccgtggt ttcatttaca cagctaaaca gaacacctgc agtagcagcg
      781 ctggaacttt cccggatctg gaaggcatcg aatgcgaaac catcgaagtc attgcaagtg
      841 gtacccgaac cgaacgtaac ccgaaagcgc acttccggtt gtccggctaa ctcacgtacg
      901 caatggcggg cggttaccca atcgccgctg ccattcccgc cgcgacagga acctgaagtc
      961 ggttgcacgt taccggacca accggatccc ggattgacaa acccggaaag attattgatg
     1021 cctgaacggt tgaaccaatt ttccgagagg caagtaccgg attcattcgc gttgccaaca
     1081 ttcacccaag aggaaccccc atcgatcgag tattgcaaat tggcgccgtc gtattgatac
     1141 tcggtttccc agaaaaccat caaggagact tccgggaact gtaatccgga aaaatcgaaa
     1201 cagggacttt ccacccacga tcgttccccg aagttgtaaa aagaaagcgt tagtccgccg
     1261 gtaatccagg aattcaaacc gtcgccggcc gtacgaataa ccggtttgcc cggagtaccc
     1321 agcgcccagt catcattcga gccactgctg ctccaccctc cgttaccggc ttcaaaactc
     1381 tcgaaatagg gaaaactact gacagtggaa gcgcattgcg cgttcaacca tttcggaaaa
     1441 gcggaaatta gcccgaaaac gagtaaaata ttgtatatca gcttcttgct cgccattccg
     1501 tgctgcaaaa aaacggaaaa tgcgcgaatc ttcccaacgc cggatgttaa ggaattaaga
     1561 aacgccgccc atgcaggcgg cgtttctctt tcttgattaa aggcctggta ccggccggtc
     1621 tttcgggtgt ttgaccatgt agttgaactt gcggttttcc gtttggttgg
//
LOCUS       DAOTON010000693         1670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_56773_length_1670_cov_2.759752, whole genome shotgun sequence.
ACCESSION   DAOTON010000693 DAOTON010000000
VERSION     DAOTON010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56773_length_1670_cov_2.759752"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="P5292_11825"
     CDS             <1..528
                     /locus_tag="P5292_11825"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HRS39859.1"
                     /translation="LNRVLPDDIVALRLLPVATDAHARFDAVERVYQYRIIHRKDPFK
                     RFRYTYLPYFPDYSMMNRVARSLIGKHDFSCFSKAHTQTLTNDCTVNRAVWKEEPGGT
                     YCFEISADRFLRNMVRAVVGTLLEVGQGRMNEPEFQALLQEGNRQDAGKSMPAHGLSL
                     VSVRYPYVQDGRKDG"
     gene            500..>1670
                     /locus_tag="P5292_11830"
     CDS             500..>1670
                     /locus_tag="P5292_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRS39860.1"
                     /translation="MFRMGGRMADQPVAGRAFDVNILKRIFRFTKPYRKGFYLSVGLT
                     LLLAVLGPLRPMLVQYTVDTYISRFDGQGLLYMSLILVGLLLAQSAVQYFHTYLTNWI
                     GQAVIRDLRIELFRHILRLRLKFFDRTPIGTLVTRSISDMETIADIFSEGLIVIIGDL
                     LQLVVIIVFMFAIDWKLSLISLSTIPILLFATRFFQKGIRDSFRDVRTQVAALNSFVQ
                     EHLTGMSIVQVFNREDEELRRFKQINREHRSANIRSVWYYSIFFPVVEILTAASIGLL
                     VWWGARGVIQETASVGNVIAFIMYINMIFRPIRELADKFNTLQMGVVSSERVFRLLDQ
                     QEFISDKGTGSADHIRGSIEFRNVWFAYKDPAPDADPAEPDWILRDLDLNIEAGKM"
BASE COUNT          366 a          422 c          471 g          411 t
ORIGIN      
        1 ctcaaccggg tgctcccgga cgacatcgtg gcgcttcgct tgttgccggt agcgacagac
       61 gcgcatgccc gattcgatgc ggtggaacgt gtttaccagt atcgaatcat acaccgcaag
      121 gatccgttca agcgtttccg gtacacttat cttccttact tcccggatta ttccatgatg
      181 aaccgcgttg cccgttcact gatcgggaaa catgacttca gttgtttcag caaggcccat
      241 acgcaaacac tgaccaacga ttgcacggtc aaccgggctg tttggaaaga ggaacccggc
      301 ggaacgtatt gcttcgagat cagcgcggat cgttttttac gcaatatggt gcgcgcagtc
      361 gtaggtaccc tgctggaagt aggacaagga agaatgaatg aaccggagtt tcaggctttg
      421 ttgcaggaag gcaatcgtca ggatgccgga aagtcgatgc ctgcgcacgg tctgagtctg
      481 gtgtcggtca ggtatccgta tgttcaggat gggaggaagg atggctgatc aacccgtagc
      541 cggcagggcg tttgacgtga acatcctgaa gcggatcttc cggtttacaa agccatatcg
      601 taaaggtttc tacttaagtg taggcctgac cttgctgctc gcggtgctgg gtccattgcg
      661 cccgatgctg gtgcagtaca cagtcgatac ctatatcagt cggtttgacg ggcagggact
      721 gttgtacatg agcctgatcc tcgtgggatt gctgcttgca cagtcggccg tacagtattt
      781 ccatacttat ctgaccaatt ggatcggcca ggcggtcata cgggacctgc gcatcgagtt
      841 gtttcggcat atactcaggc tccggctgaa gtttttcgat cgtacaccga tcggtacgct
      901 ggtaacccgc agcatcagcg atatggaaac catcgccgat atcttctcgg aaggcctgat
      961 tgtcatcatc ggcgacctgt tgcaactggt ggtgattatt gttttcatgt ttgcgattga
     1021 ctggaaactc agcctgatca gtttgtcgac gatcccgatc ctcttgttcg caacccgttt
     1081 tttccagaaa gggatacgag actctttccg ggacgtacgg acgcaagtgg cggcgctcaa
     1141 tagttttgtc caggagcatc tgacgggcat gagcatcgta caggtcttta accgggagga
     1201 cgaagaacta cgccgcttca agcagatcaa ccgggaacac cggtcggcca atatccgatc
     1261 ggtctggtac tactcgattt tttttccggt ggtcgagatc ctgaccgccg catcgatcgg
     1321 attgttggtc tggtgggggg ctcgcggggt catacaggag accgcatcgg tcggtaatgt
     1381 gatcgccttc atcatgtaca tcaacatgat cttccggccc atccgggaat tggcggataa
     1441 gttcaatacc ctccaaatgg gagtcgtaag ttccgagcgc gtcttcagac tgctggatca
     1501 gcaggagttc atttctgaca agggaaccgg ttcagccgat catatccggg gaagcattga
     1561 attccggaac gtctggtttg cttacaaaga tccggcgccg gatgcagacc ctgccgaacc
     1621 ggactggatc cttcgtgacc tcgatctgaa catcgaagcc gggaaaatga
//
LOCUS       DAOTON010000694         1669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_56822_length_1669_cov_2.654275, whole genome shotgun sequence.
ACCESSION   DAOTON010000694 DAOTON010000000
VERSION     DAOTON010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56822_length_1669_cov_2.654275"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="P5292_11835"
     CDS             complement(<1..1314)
                     /locus_tag="P5292_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRS39861.1"
                     /translation="MKKLLLLLLAFCSVAVAAAGQQLVRYELNITDTVVNYTGKSRPA
                     IAVNGMIPMPTLYFTEGDTAEVVVTNHMHHETSIHWHGLLLPNEQDGVPYLTTAPILP
                     HGTHTFRFVIRQHGTYWYHSHTMLQEQVGMYGAIVIYPRFRKPERDAVLLLSDWSDMD
                     PDQIERGLHFATDWFMIRKNAVQSYGEALVVGQLGTKLENEWKRMHAMDVSDVYYERF
                     LVNGRSDASYPDVRKGEKLRLRVINGSSSSYFWLQFGGGKFRVVATDGVDVEPVEIDR
                     LIVGVSESYDVEITIPDDGRSYGFTATAEDRTGSVTAWFGQGEHVAPTKLPVLDYFAG
                     MRMMNDMMTTGGNLHDMGMNMSLQKMDMNNVMYRERYDTLVTLHYGMLRSPVKTLLPD
                     APITEYRFELTGNMNRYVWTVNNRVISEHDKILIRSGQNVRIVITN"
     gene            complement(1316..>1669)
                     /locus_tag="P5292_11840"
     CDS             complement(1316..>1669)
                     /locus_tag="P5292_11840"
                     /inference="COORDINATES: protein motif:HMM:NF023255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HRS39862.1"
                     /translation="DSASDRAEEFVRMMGEMPVNQLDAARQKSWKLRATKIRDEADKI
                     GATRDLARQRDLFGGFSEQFYNLLEVLDLHQDTVYLQYCPMKKAYWMSAEQEIRNPYY
                     GKSMLTCGTVKDSLK"
BASE COUNT          329 a          506 c          454 g          380 t
ORIGIN      
        1 gttcgtgatc acaatcctta cgttctgccc cgaccgaatc aggatcttgt cgtgttccga
       61 gatcacgcgg ttgttcaccg tccagacgta ccggttcatg tttcccgtca gctcgaaacg
      121 gtattcggtg atgggcgcgt ccgggagaag ggtcttgacc ggtgagcgca gcataccgta
      181 gtgcagtgtg accaaggtat cgtaccgctc gcggtacatc acattgttca tgtccatctt
      241 ctgtagcgac atgttcatac ccatatcatg cagatttcct cccgtcgtca tcatgtcgtt
      301 catcatccgc atgccggcga aataatccag cacgggcagt ttcgtcggcg cgacgtgttc
      361 gccctggccg aaccaggcgg ttacggatcc ggtcctgtcc tccgcagtgg ccgtgaagcc
      421 gtatgaacgc ccatcatccg gtatggtgat ctccacgtcg taactctccg agaccccgac
      481 gataagccga tcgatttcca cgggctccac atccacacca tcggtggcta cgaccctgaa
      541 cttcccgccc ccaaactgca gccagaaata cgagctggag ctgccgttga tcacgcgcaa
      601 cctgagcttc tcgcctttcc gcacatccgg atacgacgca tcggaccttc cgttcaccag
      661 gaaacgctcg tagtacacgt cgcttacatc catcgcgtgc atccgcttcc attcgttctc
      721 cagcttggtc ccgagttgtc ctacgaccag agcctccccg taactctgca ccgcgttctt
      781 cctgatcatg aaccagtcgg tggcgaaatg aagtccgcgt tcgatctggt cggggtccat
      841 gtcggaccag tcgctgagta acaggaccgc gtcacgttca ggtttacgaa agcgcggata
      901 gatgacgatc gcgccgtaca tgcccacctg ttcctgcaac atcgtgtgag agtggtacca
      961 gtacgttccg tgctgccgga tgacaaatcg gaacgtatgc gtgccgtggg gcaggatcgg
     1021 cgccgtggtc aggtagggaa cgccgtcctg ctcgttgggc agcagcaacc cgtgccaatg
     1081 gatggaagtc tcgtgatgca tgtggttcgt cacgaccact tccgctgtgt cgccttcggt
     1141 aaagtaaagg gtgggcatgg ggatcatgcc gttcaccgcg atcgccggac ggcttttccc
     1201 ggtgtaattg accaccgtat cggtgatgtt caattcatac cggacgagtt gctgaccagc
     1261 cgcggcgacc gcaacagagc agaaagcgag caggaggagg aggagctttt tcatcttact
     1321 tgaggctgtc ctttaccgta ccgcaggtaa gcatcgactt accgtaatag ggattccgga
     1381 tctcctgctc ggcactcatc caatacgcct tcttcatcgg acaatactga aggtatacgg
     1441 tatcctgatg caggtccaag acttccagca ggttgtagaa ctgttccgag aatccgccga
     1501 agaggtcgcg ctgccgagcg aggtcccggg tggcgccgat cttgtcggct tcatcgcgga
     1561 tcttggtcgc gcgaagtttc caggatttct gccgggccgc gtccaactga ttcaccggca
     1621 tctcgcccat catccggaca aactcttccg cccggtcact ggccgagtc
//
LOCUS       DAOTON010000695         1667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_56917_length_1667_cov_2.617866, whole genome shotgun sequence.
ACCESSION   DAOTON010000695 DAOTON010000000
VERSION     DAOTON010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56917_length_1667_cov_2.617866"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1667
                     /locus_tag="P5292_11845"
     CDS             <1..>1667
                     /locus_tag="P5292_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRS39863.1"
                     /translation="RKLDSIPALVNFARPKYAPVNSAGLILSQGDRAMKSDSTRKLFR
                     LDGDGIRVGVISDSYNTLPGNPAGNDIVNRDLPGAGNPVNANPVNVMLDYPYGRGLDE
                     GRAMLQIVHDIAPKAKLSFRTGFITAGDMAEGIHALAEQDSCDIIVDDVTYITEPYFQ
                     DGIVSRAVNEVRDQGVAYFTSAGNFGGVSYESYYNPMQGPASIPGMVHDFGGGDYLQA
                     ISLKRGTYTLVMQWDDSLYSLGQLPGARNNFDIYLTDLTGTKLFGLNRQNNWGDPIEV
                     LPFTVTADVLANIVITRTWTNDPMGPPMKIKYIIFRGDATILEHQAGSSTIIGQANCD
                     GAMSVGAVRYTKTPAFGQVPEPESFTSRGGTAVRGVVRNKPDFSAPNGVNTSVNFFSR
                     DLESDGLYNFYGTSCAAPHAAGAAALIQQAAKKFNDAKLSPDSLRNLMKATAVDIGGD
                     GFDLTTGAGLINPMAAVRTFARPLPQVDTVIPDLPSAVFGQDPVSVTVVGDHFVNGAE
                     VYLDGTALPTVYQDEQHLLTEIPVFTGNPMLQVFNSPSTSNGTDGGY"
BASE COUNT          357 a          514 c          443 g          353 t
ORIGIN      
        1 gcgcaagctg gattcgatcc cggcgctggt caacttcgcc cgaccgaagt acgcaccagt
       61 caatagtgcc ggtctcatcc tctcgcaggg cgaccgcgcc atgaagtccg attccacccg
      121 caagctcttc cgcctcgatg gcgacggcat tcgggtaggt gtgatctccg acagttacaa
      181 tacgcttccc ggtaatccgg caggcaatga tatcgtgaac cgcgacctcc ccggtgccgg
      241 caacccggtt aacgccaacc cggtcaacgt catgctcgat tatccgtatg gacgtggtct
      301 tgacgaaggt cgtgccatgt tgcagatcgt acacgatatc gcgcccaaag cgaaactttc
      361 cttccgcacc ggtttcatca ccgccggcga catggctgaa ggtatccacg cactggcgga
      421 gcaggatagc tgcgacatca tcgtggacga cgtcacctac atcaccgaac cttacttcca
      481 ggacggtatc gtttcccgcg ccgtcaacga agtgcgcgat cagggtgttg cctactttac
      541 ttcagccggt aacttcggtg gggtatccta cgaatcctac tacaacccga tgcagggacc
      601 tgcaagcatt ccgggcatgg tccacgattt cggtggagga gattatctcc aggccatcag
      661 cctgaagcgc ggtacctaca cgctggtcat gcaatgggat gattccctgt attcactcgg
      721 acaattgccg ggtgcacgga acaactttga catttacctc accgacctga ccggtaccaa
      781 attgttcgga ttaaaccgcc agaacaactg gggtgacccg atcgaagtac tgccctttac
      841 cgtaacggcg gatgtgttgg ccaacatcgt gatcacccgc acctggacca acgatccgat
      901 gggcccgccg atgaagatca agtacatcat cttccgcggc gacgctacca ttctggagca
      961 ccaggccggc agctcgacca tcatcggaca agccaactgt gatggagcca tgtctgttgg
     1021 cgcggtgcgc tataccaaga cccccgcgtt cggccaggtg ccggaaccag agagctttac
     1081 ctctcgcggt ggtacggcag ttcgcggcgt cgtgcgtaac aaaccagact tcagcgcacc
     1141 aaacggtgtc aacacgtcgg tcaatttctt ctcgcgcgat ctggagagtg acggcctcta
     1201 caacttctat ggtacttcct gcgcggctcc tcatgccgcc ggtgcagccg cgctgatcca
     1261 gcaagccgcc aagaagttca acgatgcgaa actctcaccc gattccctcc gcaacctgat
     1321 gaaggcaacg gcagttgata tcggaggcga cggtttcgat cttacgaccg gtgcaggcct
     1381 catcaacccg atggccgccg tacgcacatt cgcgcgtccg cttccgcagg tcgatacggt
     1441 cattccggac ctgccctcgg ctgtgttcgg acaagatccg gtttcagtaa cggtggtcgg
     1501 tgaccacttc gtgaatggag cggaggttta tcttgatgga acagcactgc cgaccgttta
     1561 tcaggacgag caacacctgc tgacagagat tccggtcttc accggtaatc cgatgttgca
     1621 ggtattcaat tcgccttcta cctccaatgg aactgacggc ggttatt
//
LOCUS       DAOTON010000696         1661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_57188_length_1661_cov_3.739726, whole genome shotgun sequence.
ACCESSION   DAOTON010000696 DAOTON010000000
VERSION     DAOTON010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57188_length_1661_cov_3.739726"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="P5292_11850"
     CDS             complement(<1..701)
                     /locus_tag="P5292_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRS39864.1"
                     /translation="MSLFIKKPLDHVLAQAADNEKGLKRTLGAANLVALGIGAIIGAG
                     LFVRTAAAAGQAAGPGVTLSFVVAAIGCAFAGLCYAEFASMIPIAGSAYAYSYVTMGE
                     VVAWTIGWALIMEYALGAATVSIAWSEYLNKLLDGAIPYELCHSPFESFTDASGVLHQ
                     GLLNAPALIILLVLTLLLIRGTQESALVNTIIVFIKVAIVLAFIAIGWKFINPANYAP
                     YLIPEGTPPVTDAAG"
     gene            complement(988..1398)
                     /locus_tag="P5292_11855"
     CDS             complement(988..1398)
                     /locus_tag="P5292_11855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HRS39865.1"
                     /translation="MANKDRYLYIRRSRIPGAGKGLFTKVDIEKGERVTEYKGKLQRW
                     SEVKDEDGYNAYIFKINSRWAVNALPAVKTLGRYANDARGLSRVEGLRNNSEYDTEGK
                     KVYIVATRKIRKGEEVLVDYGAAFWRLIRKIRGS"
     gene            complement(1458..>1661)
                     /locus_tag="P5292_11860"
     CDS             complement(1458..>1661)
                     /locus_tag="P5292_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39866.1"
                     /translation="VIQLFGTRLYSSARDLHQLTGIPMHMILNCPNDRQNIILDGAEY
                     LHIRKLNFLLDYPSEGEGIPPAA"
BASE COUNT          359 a          460 c          490 g          352 t
ORIGIN      
        1 ttacccgccg cgtcggtgac cggtggtgta ccctccggaa tcaggtaggg tgcgtaattg
       61 gccgggttga tgaacttcca gccgatggcg atgaatgcca gcacgatcgc caccttgatg
      121 aacacgatga tggtgttcac cagcgccgat tcctgcgtgc cgcgaatgag cagcagcgtt
      181 aataccagca ggatgatcag cgccggcgcg ttcagcaatc cctggtgcag gacgccggaa
      241 gcgtcggtga agctttcaaa aggtgaatga cagagttcgt acggtatcgc gccgtctaag
      301 agcttgttca ggtattcgct ccaggcgatg gagacggtgg cggcgccgag ggcgtattcc
      361 atgatgagcg cccagccgat ggtccaggcc acgacttcgc ccatggtcac atacgaataa
      421 gcatacgcgc tgccggcgat cgggatcatc gaggcgaact cggcgtaaca caatccggcg
      481 aatgcgcagc cgatcgcggc cacaacgaac gacaaggtga cgcccggtcc ggcggcctga
      541 ccggcagccg cggcagtgcg cacgaacagg ccggcgccga tgatcgcgcc gatgcccaac
      601 gcaaccagat tggccgcgcc cagcgtacgc ttcaggccct tctcattgtc agccgcctgc
      661 gccaggacgt ggtccagggg tttcttgatg aacagactca tacggttgtt ttgatttgaa
      721 tggggaaagt aggaaaaagt tcgcaaaagc ggtttcgtgt ttcgggtttc cagtttccgg
      781 tttccggttg tgttaattga gtttgagtga acacttaagt atttagtccg atagtctatc
      841 cttacatgag atcgtgataa agcgtttccg attagaatat ggataacaac caataaagaa
      901 caggaaactg gaaacccgaa acccgaaacc ggacccaacc tacaaccaat aaccaaccgg
      961 aaactcgaaa ccggaaaccc gaaaccatca actccctcgg atcttccgaa tgagcctcca
     1021 aaacgccgct ccataatcca ccagcacctc ctcgcccttt cggatcttgc gggtggcgac
     1081 gatgtagact tttttgcctt cggtgtcgta ctcgctattg ttgcggaggc cttcgacgcg
     1141 gctgaggccg cgggcgtcgt tggcgtagcg gcccagggtc ttgacggccg gcagggcgtt
     1201 caccgcccag cggctgttga tcttgaagat gtaggcgttg taaccgtctt cgtcttttac
     1261 ttccgaccag cgctgtaact ttcctttgta ctcggttacg cgttcgcctt tttcgatatc
     1321 gactttggtg aagagtcctt tgccagctcc cggaatgcgc gagcggcgga tgtagaggta
     1381 acggtctttg ttcgccacgg cgcgaagaaa agaaaccggc ggagaaaaac cgcgccttac
     1441 gggacgcggt cggtacatca ggctgccggc gggatcccct cgccttcgct cggatagtcg
     1501 agcaggaagt tgagcttgcg aatgtgcagg tactcggcac cgtccaggat gatgttctgc
     1561 cggtcgttcg gacaattcag gatcatgtgc atcggtatgc cggtcaactg gtgcaggtcc
     1621 ctggcggagc tgtacaaccg ggtgccgaag agctggatca c
//
LOCUS       DAOTON010000697         1659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_57284_length_1659_cov_3.804239, whole genome shotgun sequence.
ACCESSION   DAOTON010000697 DAOTON010000000
VERSION     DAOTON010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57284_length_1659_cov_3.804239"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="P5292_11865"
     CDS             <1..556
                     /locus_tag="P5292_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRS39867.1"
                     /translation="VEFGAEAWLPAMVGISIIREIGPVITALICAGKIGSSMGAELGS
                     MRVTEQIDAMEVSGTNPFKYIVVSRIVASTLMIPILVVIGDAIALFGSFLAVRVKGGV
                     SLTLYFNQVFESLEFGDVIPSFIKAFFFGFAIGLVGCYKGYFSTKGTEGVGRAANSAV
                     VIASVLIFVIDLIAVQITEFLGLI"
     gene            556..1305
                     /locus_tag="P5292_11870"
     CDS             556..1305
                     /locus_tag="P5292_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS39868.1"
                     /translation="MAASSTNEQPIIEIRDMHKSFGKNHVLRGFNLTLHRGENVVVLG
                     RSGCGKSVLIKCIVGLIPYDRGSLKVFGKEVSEMSIRELDEVRAKIGFVFQSSALYDS
                     MTVRENLEFPLRRHERDHRHDSYIELVSNTLENVGLKHAIELMPSELSGGMRKRIGLA
                     RSLIMQPEIMLYDEPTTGLDAITGKEISNLMLDMQRKYNTSSIIISHDMYCARVVGNR
                     LVALIDGINYQEGTFSELSSSKDPMVRAFFE"
     gene            1309..>1659
                     /locus_tag="P5292_11875"
     CDS             1309..>1659
                     /locus_tag="P5292_11875"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRS39869.1"
                     /translation="MAKETFNNIRLGVFVITGLILLVAGLYFIGNNKNLFGDRFELYA
                     TFDNVSGLQKGNNVRFSGIDVGTVDEITILNDTSLRVKMVINQDLLHHIRKNSYAAIG
                     TDGLMGNKLININPG"
BASE COUNT          387 a          464 c          441 g          367 t
ORIGIN      
        1 ggtcgagttc ggtgcggaag cctggctacc ggccatggtg ggcatctcca tcatccggga
       61 gatcgggcct gtgatcacgg cgctcatttg cgcgggcaag atcggctcca gcatgggagc
      121 ggaactgggc tccatgcgcg taacggaaca gatcgacgct atggaagtat caggcacgaa
      181 tcctttcaaa tatatcgttg tatcacgcat cgtggccagt acgctgatga tccccatcct
      241 ggtcgttatc ggtgatgcca tcgcgctctt tggttccttt ctcgccgttc gggtaaaagg
      301 gggtgtcagc ctgaccctct acttcaacca ggtgttcgaa tcactcgagt tcggagacgt
      361 cattccctcc ttcatcaagg ctttcttctt cgggttcgcc atcgggttgg tcgggtgtta
      421 caagggttac ttttctacga aaggaaccga aggcgttggt cgggccgcga actcggccgt
      481 cgtgattgcc tccgtcctca tcttcgtgat cgacctgatc gctgttcaaa taaccgaatt
      541 cctcggactc atctgatggc agcctcctcc accaacgaac aaccgatcat cgagatccgc
      601 gacatgcaca agtcgttcgg gaaaaaccat gtactgcgtg gcttcaatct gaccttgcat
      661 cgcggagaaa acgtcgttgt gctcggaagg tcaggctgcg gcaaatccgt actgatcaaa
      721 tgcatcgtcg gattgatccc atacgaccgc ggcagtctga aagtattcgg caaggaagtc
      781 agcgagatgt cgatccggga gctggacgaa gtacgcgcca agatcggctt cgtttttcag
      841 agcagcgcgc tgtatgattc gatgaccgtt cgtgagaacc tcgagttccc gttacgtcga
      901 cacgaacgcg accaccgcca cgattcgtac atcgaactgg tcagcaatac cctggaaaat
      961 gtcgggctga aacacgcgat cgaactgatg ccgtcggagt tgtcgggggg tatgcgcaaa
     1021 cggatcggac tggcccgctc gctgatcatg cagccggaga tcatgctgta cgacgaaccc
     1081 accaccggac tggacgcgat caccggcaag gagatcagca acctcatgct cgacatgcaa
     1141 cggaaataca acacatcctc catcatcatc tcccatgaca tgtactgcgc gcgcgttgtg
     1201 ggaaaccggc tggtggccct gatcgacggc atcaattacc aagaaggaac tttttccgaa
     1261 ctttccagct ccaaagaccc gatggtgcgg gcctttttcg aataatccat ggcaaaagag
     1321 accttcaaca acattcggct gggtgttttc gtcatcaccg gactgatctt gctcgtagcg
     1381 ggactctatt tcatcgggaa caacaagaac ctcttcggtg atcgatttga actatacgcg
     1441 actttcgata atgtcagcgg cctgcaaaag ggcaacaacg tacggttctc gggcatcgac
     1501 gtcgggacgg tcgacgagat caccatcctg aacgatacca gtcttcgggt aaagatggtc
     1561 atcaatcagg acctcctgca ccacatccgg aaaaactcgt atgcagccat cggaaccgat
     1621 gggttgatgg gcaacaaact catcaacatc aaccccggc
//
LOCUS       DAOTON010000698         1659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_57293_length_1659_cov_3.017456, whole genome shotgun sequence.
ACCESSION   DAOTON010000698 DAOTON010000000
VERSION     DAOTON010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57293_length_1659_cov_3.017456"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="P5292_11880"
     CDS             complement(<1..244)
                     /locus_tag="P5292_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRS39870.1"
                     /translation="MARTPSTMVELGTPLPAFSLPDTVTHRTYQDSQFLGKPLVVAFI
                     CNHCPYVQLIEAELMNIARSYGARGISFLAISSNDPV"
     gene            361..433
                     /locus_tag="P5292_11885"
     tRNA            361..433
                     /locus_tag="P5292_11885"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:394..396,aa:Lys,seq:ttt)
     gene            complement(462..1625)
                     /locus_tag="P5292_11890"
     CDS             complement(462..1625)
                     /locus_tag="P5292_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39871.1"
                     /translation="MKISEVTTPAEAEAFIRFPIRLYRGDPNYLQPLDKDIKAVFDPK
                     ENKFFRHGQCTRFLLRDNDGQVIGRIAAFINDRLAKKESQPTGGCGFFECIHDQAAAN
                     VLFDTARDWLAARGMEAMDGPINFGERDAWWGLIVEGFDPPPYKMNYNPPYYRELFES
                     YGFQIYFQQWCYSLKVANRLQEKFYARHAAIRENPDYRAEHLRKSRLEKYAEDFRTVY
                     NKAWVKHGAGKELESRQVQGFFRKMKPVIDELAIWYVYYKNEPVAMWVNLPDINQLFG
                     RFKGKFGLLEKLRFVWMLKRRKTNKFIGLVFGIVPEHQGKGVDSFMIVEGASVIQGKK
                     LYEDFEMQWIGDFNPKMVAIAESLGTYRSRVLITYRYLFDRTKPFQRHPILYK"
BASE COUNT          374 a          421 c          442 g          422 t
ORIGIN      
        1 gcaccggatc gttggagctg atcgccagga aggagatacc acgtgcgcca tacgaacgcg
       61 cgatgttcat gagttctgct tcgatcaatt gaacatacgg acagtgattg cagataaagg
      121 ccactaccag tggctttccc aggaattggg aatcctgata ggtgcgatgg gtgaccgtat
      181 ccggaagtga aaaagcgggg agtggtgtcc ccaactcgac catggtggac ggtgtgcgcg
      241 ccataaacta ttgttaatca gctaatgtac ctcagcatga ggcaagtgag aaaacgaaaa
      301 aattttttcc tgaaggtcgt ttgatttaaa aaactgctat ttttgcagcc tctttaaggc
      361 gatttcgtag ctcagttggt agagcaatac acttttaatg tatgggtcct gggttcgagt
      421 cccagcgaaa tcacaaaaaa ggcctccctc cggaggcctt tttacttgta caagatcggg
      481 tgtcgttgga atggtttggt ccgatcgaac aggtagcgat acgtgatcag aacccggctg
      541 cggtaggttc caaggctttc tgcgatcgcg accatcttcg gattgaaatc tccgatccat
      601 tgcatctcga agtcttcata gagtttcttg ccctggatca cactcgctcc ttccacgatc
      661 atgaaggagt cgacaccttt accttggtgt tcaggtacga tcccgaatac gaggccgatg
      721 aatttgttcg tcttcctgcg cttgagcatc cagacgaaac gtagtttttc cagtaacccg
      781 aatttgcctt tgaaccggcc gaagagctgg ttgatgtcgg gaaggtttac ccacatcgcc
      841 accggttcgt tcttataata gacataccag atggccagtt catcgatgac gggtttcatc
      901 tttcggaaaa agccctggac ctgccgggat tccagttctt ttccagcgcc gtgcttgacc
      961 caggccttgt tatagactgt tctaaaatcc tccgcatact tttccagccg gctcttccgc
     1021 aggtgctcgg cacgatagtc cggattctcc ctgatcgcgg catgtctggc atagaatttt
     1081 tcctgcagcc ggttggccac cttcagtgaa tagcaccatt gctggaagta gatctgaaag
     1141 ccgtacgact cgaataattc ccggtaatac ggcgggttgt agttcatctt gtaaggtggc
     1201 ggatcgaatc cttccacgat gaggccccac caggcatccc gttcgccgaa attgatgggt
     1261 ccgtccatgg cctccatacc gcgagctgcc agccagtcac gtgcggtatc gaacagcacg
     1321 ttcgcggctg cctggtcgtg gatgcattcg aagaatccac atccaccggt gggctggctt
     1381 tctttctttg ccaaccggtc gttgatgaag gcggcgatcc tgccgatgac ctggccgtcg
     1441 ttgtcgcgca acaagaaccg ggtacactga ccatgtcgga aaaacttgtt ctccttcgga
     1501 tcaaagacgg ctttgatgtc cttgtccaac ggctgcaagt agttcggatc gccccggtaa
     1561 agacgtatgg ggaagcggat gaacgcttca gcctccgctg gcgtggtgac ttcactgatc
     1621 ttcatcgggt acgaatgtag gaaatggagg ggagcggtg
//
LOCUS       DAOTON010000699         1657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_57409_length_1657_cov_2.716604, whole genome shotgun sequence.
ACCESSION   DAOTON010000699 DAOTON010000000
VERSION     DAOTON010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57409_length_1657_cov_2.716604"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="P5292_11895"
     CDS             complement(<1..683)
                     /locus_tag="P5292_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRS39872.1"
                     /translation="MGRIFRNLFGIYALIVFTLSVLVVLPGYFLVFLLASKERAPRLA
                     HRLISRSWASVLFLLFGIRVKVRNSQVLDPEKTYVFAANHRSLLDIPSYAIACQNTFR
                     FLSKEELTKIPGMGWIIRKLYITVDRKDKAARLRSMDRMVQSLRDGVSVFLCPEGTRN
                     KTNRPLLDFHDGAFRVAIEAQVPLAVMTIIHSDTLLSPLHPIALMPGRIECIWEHPIE
                     TSGMTVQDVP"
     gene            complement(1510..1596)
                     /locus_tag="P5292_11900"
     CDS             complement(1510..1596)
                     /locus_tag="P5292_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39873.1"
                     /translation="MVELDGGHIRRMIEKPSDPPTNLAIVGE"
BASE COUNT          365 a          431 c          446 g          415 t
ORIGIN      
        1 gggacgtctt gtacagtcat cccactggtc tcgatcggat gctcccagat acattcgatc
       61 ctgccaggca tcagtgcaat cgggtggaga ggagaaagca gcgtgtcgga atggatgatc
      121 gtcatgactg ccaagggcac ctgggcttca atggctacgc gaaatgctcc gtcatgaaaa
      181 tcgagcaggg gtcggttggt tttgtttcgg gtcccttccg gacaaagaaa tacggagact
      241 ccgtctcgca gggattgaac cattcggtcc atgctgcgta atcgggcagc cttgtccttg
      301 cggtccacgg tgatgtagag ctttcggata atccacccca tgccggggat tttggtcaat
      361 tcttccttcg ataaaaagcg gaatgtgttc tggcaggcga ttgcatacga aggtatatcc
      421 aacaaggagc gatgattggc tgcgaagacg tacgtttttt cgggatcaag tacctggctg
      481 tttcttactt tgacccgaat gccaaagagg aggaagagca cagacgccca ggagcgggag
      541 atgagcctat gggccaatcg aggcgctcgt tccttgctgg ccaatagaaa taccaggaaa
      601 tagccgggaa gtacaacaag tactgaaagc gtaaaaacga tcagcgcgta aatgccaaaa
      661 aggttgcgaa agatccggcc catttcgtag aatctgcaca aacatacgct cttttgaact
      721 ccgctgtttt cgacaatcat ccttatccct ttttttcgtt tatttgatct gccaaccatc
      781 aactttccca tgcgtttatc tgtactgatt gcagccctcg cgctggctat tccttgcttt
      841 gcgggacatc ccgtcgttcc cggtaacgag atccgacttg cttccggaac cattaccacg
      901 gtgcaggatt tcagtgatga atcccttagc cgtatttcat cgcaggatct gttcgacgat
      961 cgattttatc ggatcataca gttcaacagc ctccccgatc cggcacgaaa gcgtcaactt
     1021 gaaaccgcgg gtcttcgctt cgtctcctat cttcctgcca atgcctggct ggtctcgctg
     1081 ccgcgttcag tggacagccg cttgctgacc aagacgaacg ttcgtactgt actcccggtc
     1141 gatgaacgct acaaattgaa ccgacgggtg gcggaaggca gttttccgga tcatgctatc
     1201 cgtgccggcg gacgcgtgga attgcagttg agcttttttc cggatgtttc attcgaacac
     1261 gcgaccgcgg tattgcaaca attcggagcc aggatcaccg ctgctcaatc gggcttcggt
     1321 ctggtaaacg tggaattccc aaaggaggat atccatcgtc tggtgcgact tcctttcgtg
     1381 cggtacattg agccggttgc tcccgcttct tatcctgaag atagccgggg ccgttcactg
     1441 caccggagca attatatcaa cagtgattta ccatcaggct tgcattataa tggcgacggt
     1501 gtcgccatct cactcgccga cgatggcgag gttggtgggg ggatcggagg gtttttcgat
     1561 catccggcgg atgtggccgc cgtcaagctc gacgatgccg aagcgtttgg ggtcatcgac
     1621 ttccttgacg ccgatccaat tggagtccga gccgatg
//
LOCUS       DAOTON010000700         1656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_57458_length_1656_cov_3.386633, whole genome shotgun sequence.
ACCESSION   DAOTON010000700 DAOTON010000000
VERSION     DAOTON010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57458_length_1656_cov_3.386633"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="P5292_11905"
     CDS             complement(<1..389)
                     /locus_tag="P5292_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRS39874.1"
                     /translation="MANRLKSEVAEETTEPKESREKEKRTRKPKSSSKSRWESVRNFF
                     LDERTHKVFGLLMLAGAFFTLLAFISNLVTWKEDQAIAGIDSVWQLLTRQDIQAENWM
                     GKLGAITALQFQNAWFGICAFLLPFYLT"
     gene            651..1322
                     /locus_tag="P5292_11910"
     CDS             651..1322
                     /locus_tag="P5292_11910"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HRS39875.1"
                     /translation="MKKLLSFLFVSLVACTSVFAQWENKIMQGKVVFDISYPNSKMDQ
                     QTLAMLPTQSIMYFKGQMSRVEVSMPMGTNVVISDHKLGEGTMLMDMMGNKMAIAMNK
                     ADMENERSSMPKPVVKQSSDTKVIAGHTCNKATVTQEGKDGKVSYDVWYTQDLLANNS
                     FASTVDGIDGFMLEFETIQNGMSMKMSARSIEELQVDDSQFMIPDGYQRTTWKEMKEN
                     MKGVK"
     gene            1440..>1656
                     /locus_tag="P5292_11915"
     CDS             1440..>1656
                     /locus_tag="P5292_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS39876.1"
                     /translation="MLHRLNIRNYALIDELELQLEEGMNTITGETGAGKSILLGALSL
                     ILGQRADNTALSDKSQKCIVEGEFRLPR"
BASE COUNT          436 a          406 c          415 g          399 t
ORIGIN      
        1 gtaaggtaga aggggagcag gaacgcacaa atgccaaacc aggcgttctg gaattgcagg
       61 gcagtgatcg cacccagttt ccccatccag ttctctgcct ggatgtcttg tctggtcagt
      121 aattgccaga ccgaatcgat ccccgcgatt gcctggtctt ccttccaggt caccaggttg
      181 ctgatgaatg ccaacagcgt aaaaaacgcc cctgccagca tgagtaatcc gaagacctta
      241 tgggtccgtt cgtccaggaa gaaattgcgc acgctttccc aacggctctt agaggagctc
      301 ttgggtttgc gggtacgctt ctctttttcg cggctttctt tcggttccgt cgtttcctca
      361 gccacttcgc ttttcagccg attcgccatg aattctgcga tttttatagt ttttaaaagt
      421 agaattgacg cccggataag gggatcgagg tagttaaaag atatgaacag tgttttaccg
      481 gaaattgaac ggtatcatgg cctacggtat tgaaggagtg taagatatag gaacgaacca
      541 ttcacaaccg attgttggac gtccaccaaa aagccatggg gtgcacccgt tttcattgcc
      601 gaaagaaggc ttatatttgt agtaaacctc aacgccaaac atccatcacg atgaaaaaac
      661 tcctctcttt cctgttcgtt tccctggtcg cctgcacctc cgttttcgcc cagtgggaga
      721 acaagatcat gcagggcaaa gtcgtttttg acatttcgta cccgaacagc aaaatggatc
      781 agcagactct ggccatgttg cccacccaat ccatcatgta cttcaaaggc cagatgtcgc
      841 gtgtggaagt ttccatgccg atgggtacca acgtggtcat ttccgaccat aaactcggcg
      901 aaggaaccat gctgatggat atgatgggaa ataaaatggc catcgccatg aacaaggctg
      961 atatggagaa tgagcgtagt tccatgccca agccggttgt caagcaatcg agcgacacca
     1021 aggttatcgc cggccacacc tgcaataagg ctaccgtgac gcaggaaggc aaagacggca
     1081 aggtctcgta tgatgtctgg tacacccagg atctgctggc caataactca tttgcctcca
     1141 cggtagatgg tatcgacgga ttcatgctcg agttcgagac catccagaac ggaatgtcca
     1201 tgaaaatgag cgcacgcagc attgaagaac ttcaggtgga cgacagccaa ttcatgattc
     1261 cggacggtta ccagcgtacc acctggaaag aaatgaaaga aaacatgaaa ggcgttaagt
     1321 gattctaagg ggatccgata tacaaagccg gccaagagcc ggcttttttt atgttgttga
     1381 ttgaaattct actatttgcg gatcgataac cgcatcgcct tttgtaattt caccctgcca
     1441 tgttacaccg cctgaatatc cgcaactacg cgcttatcga tgaactggaa ttgcagttgg
     1501 aagaggggat gaatacgatc acgggtgaaa ccggagcagg taagtcgatc ctcctggggg
     1561 cattgtcact gatactgggt cagcgggccg ataacaccgc attgtccgac aagtcccaaa
     1621 agtgtattgt cgaaggtgag ttccggctac cccgga
//
LOCUS       DAOTON010000701         1656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_57469_length_1656_cov_2.407245, whole genome shotgun sequence.
ACCESSION   DAOTON010000701 DAOTON010000000
VERSION     DAOTON010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57469_length_1656_cov_2.407245"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1656
                     /locus_tag="P5292_11920"
     CDS             <1..>1656
                     /locus_tag="P5292_11920"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRS39877.1"
                     /translation="PTHPDTALAAATPTTGATFASIREAMPVLPWTGSNAFVLAPGRS
                     ASGKVLFENDTHIQHQQPAVWYEAHLECPGFRFYGNFLAGFPFAPIGRTPAYAWGLTI
                     LENDDLDFFRERPDPTDSNKVITAGQSVPLQLREEIIKVKDSADVRLVCRSGPHGPIC
                     SDVMKDFASLTKDPVAVSWTFFKFPNNLPEVTYELAHAANRNAFRDAVSKIAAPGLNV
                     LYGDSAGHIAWYTATRFVQRAPGRSAVFLLDGETGEDDWLGFYDFSYNPRSEDPEQGY
                     VFSCNHQPDSVKGVLHPGYYLPEDRASRMQQLLEAKAKHSPEDAKAIVLDVTNPKAAR
                     VAQLLAACVPDTASAPVKAAARRLQAWTGTHSLQDVEPVIYYRLLYEVLHNTLADEMG
                     EKDFEAFLSTHIEKNSLLTLLNNDSSRWWDDRNTADRSESRKEIIGKAWPVAIAQLTD
                     LYGDDQDNWHWLRAHTIEIGHPLAKQPPLHLVFGIGSYPIPGGMETINNQSFTLTGKG
                     SYSVNLGPALRRIVDFAQPESGESINPSGQSGNPSSAYYQDQA"
BASE COUNT          345 a          504 c          503 g          304 t
ORIGIN      
        1 acccgacgca tcccgatacc gcgttggcgg ccgccacacc aacgaccggc gccaccttcg
       61 cgtcgatccg ggaagcgatg ccggtactgc cctggacggg tagcaacgcg tttgtcctgg
      121 caccggggcg ctccgcttcg gggaaggtgt tgttcgagaa cgacacgcac atccagcacc
      181 agcaacccgc cgtctggtac gaggcgcacc tggagtgtcc gggctttcgc ttttacggca
      241 acttcctcgc aggatttccc ttcgcgccga tcgggcgcac gcccgcctac gcctggggac
      301 tgaccattct cgaaaacgac gacctcgatt ttttcaggga gcgacccgac ccgaccgaca
      361 gcaacaaggt gatcaccgcc ggacaatccg ttcccttgca actgcgggag gagatcatca
      421 aagtcaagga ttccgcagac gtacggctgg tctgtcgcag cggtccgcac ggaccgatct
      481 gcagcgacgt gatgaaggac ttcgcttcgc tgaccaagga ccccgttgcc gtgagctgga
      541 ccttttttaa gtttccgaat aatctgccgg aagtcacgta tgaactcgca cacgccgcga
      601 accgaaacgc attccgtgat gcggtaagca agatcgccgc gcccggattg aacgtgctgt
      661 acggcgattc ggcggggcac atcgcctggt acacggccac ccgcttcgtg cagcgcgcgc
      721 ccggtcgttc ggccgtgttc ctgctcgacg gcgagacagg cgaagacgac tggctgggct
      781 tttacgactt ctcgtacaac ccacgttcgg aagatccgga gcagggttat gtgttcagtt
      841 gcaatcacca gcccgactcg gtgaagggcg tgttacatcc gggttactat ctgcccgaag
      901 accgcgccag caggatgcaa caactgctgg aggcgaaggc gaaacactcg ccggaagacg
      961 cgaaagcgat cgtgctcgat gtcaccaatc ccaaagccgc cagggtcgcg cagttgctgg
     1021 ccgcctgtgt tcccgacacc gcatcggctc cggtgaaggc tgcagcacga cgcttacagg
     1081 cctggacggg tacgcattcg ttgcaggatg tcgagccggt gatctattac cggttgttgt
     1141 atgaagtcct gcacaatacc ctcgcggacg agatgggcga aaaggatttc gaagcgtttc
     1201 tttcgacgca catcgagaag aactcgctcc tgaccttgtt gaacaacgac agctcgcgct
     1261 ggtgggacga ccggaacacg gcggatcgaa gcgaaagcag aaaagagatc atcggcaaag
     1321 cctggccggt ggccattgcg cagttgacgg acctgtacgg tgacgatcag gacaactggc
     1381 actggctgcg cgcgcatacg atcgagatcg ggcatccgct ggcgaagcag ccgcccctgc
     1441 atctggtctt tggcatcggt tcgtatccga tcccgggcgg gatggagacg atcaacaacc
     1501 agagcttcac gctgacggga aaaggttcgt attcggtgaa cctcggacct gcgttgcggc
     1561 gcatcgtcga tttcgcgcaa ccggagtcgg gtgagagcat caatccgagc gggcaatccg
     1621 gcaatccgtc gagcgcttat tatcaggacc aggccg
//
LOCUS       DAOTON010000702         1652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_57659_length_1652_cov_3.654978, whole genome shotgun sequence.
ACCESSION   DAOTON010000702 DAOTON010000000
VERSION     DAOTON010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57659_length_1652_cov_3.654978"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="P5292_11925"
     CDS             <1..580
                     /locus_tag="P5292_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRS39878.1"
                     /translation="SDIILRFGIPSVLAALIGAFLLNFLTEIAPLHTWQWGSHTCTIT
                     PVKLVVAILLAFFSVFELVPRLRHWQVDQRYMIHGGLLSGFFGGLSGHQGALRSAFLM
                     RAGMDKETFIGTGVAIACMTDLARMSIYLGHWTQLPPETSITPIILAILSAFAGALIG
                     RRFLRKMGMKTVQNVVGIALLLFSGLLAAGIV"
     gene            654..1262
                     /locus_tag="P5292_11930"
     CDS             654..1262
                     /locus_tag="P5292_11930"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HRS39879.1"
                     /translation="MQLSPEQLSANGYRFQSSIRFESINQFVQDRLQRGGALLRLYWL
                     CVVASVFAMSHVLWKQDIVTLDLLAGGIGFGLAGFLVIVLPAHEAIHGITYKLLGAPS
                     ISFHADWKRMFFFAAADRFVLNRREFILVAAAPFILLTIGLLAAQFFTNGMLQLAIAT
                     NLVLHTGGCIGDFTLVAYSMSLGEKFCTYDDLQKQETFFYQK"
     gene            complement(1265..>1652)
                     /locus_tag="P5292_11935"
     CDS             complement(1265..>1652)
                     /locus_tag="P5292_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRS39880.1"
                     /translation="GRFFGQRALKMKIGGKALVKQEYMDRTHTFFEKYGTKAIIMARF
                     VPIVRTFTPFVAGVGRMNYKKKFLPYDIAGGFLWIASMSLAGYALGQNPWVKKHFEAV
                     VVGIIFLSILPMIITWLRHRFSGKSQ"
BASE COUNT          338 a          460 c          421 g          433 t
ORIGIN      
        1 aagtgatatc atacttcgtt tcggaatccc gtccgtattg gccgccttga tcggtgcatt
       61 cctgctgaat ttcctcaccg agatcgcgcc tttgcacacc tggcagtggg gaagccatac
      121 ctgcaccatc acaccggtaa aattggtggt ggcgatcctg cttgccttct tctccgtctt
      181 cgaactcgtg ccccggttgc gtcactggca agtcgatcag cgctatatga tccacggagg
      241 actgttgtcc ggtttctttg gtggcttgtc ggggcatcag ggagccttac gctccgcctt
      301 tttgatgcgg gcaggcatgg acaaggaaac gttcattggc accggcgtgg cgatcgcttg
      361 catgaccgac ctggcccgta tgtcgatcta tctcggtcat tggacacaac tccctcctga
      421 aaccagcatt accccgatca ttctggccat cctttccgct tttgcaggcg ctctgatcgg
      481 ccggcgcttc cttcggaaga tgggaatgaa gaccgttcaa aacgtcgtag gaatcgcgct
      541 gttgctgttt tccggcttac tcgcggcagg catcgtctga ttagagggag tacccctatt
      601 gttgattttc aggatttttc ttattttcat cctaacagga actggaacat cctatgcagc
      661 tgagtccgga acaactcagc gcgaatggct atcgctttca atccagcata cggtttgaaa
      721 gcatcaatca attcgtgcaa gatcgattgc agcgaggtgg tgcattgcta cgactttact
      781 ggctttgcgt cgttgcctcg gtatttgcca tgtcgcatgt cctctggaag caggatatcg
      841 tgaccctcga cctgctggcg ggcgggatcg gattcggact tgccggattt cttgtcatcg
      901 tgttaccggc acacgaagcc atccacggta tcacctataa attgcttggc gccccgagca
      961 tcagctttca tgccgactgg aaacgcatgt tcttcttcgc cgcggccgat cgcttcgtac
     1021 tcaaccgacg cgaattcatc ctggttgccg cagctccttt catcctgctg accatcggat
     1081 tgctggctgc gcagtttttc accaacggca tgttacagct cgccatcgcg accaacctgg
     1141 tgctgcatac cggcggctgc attggtgatt tcacgctggt agcctattcc atgtcactcg
     1201 gcgaaaaatt ctgcacctac gacgacctgc agaaacagga aaccttcttc tatcagaagt
     1261 gagtttattg cgactttcct gaaaaacggt gacgcaacca ggtgatgatc atcggaagga
     1321 tcgagaggaa aatgattccc accacgaccg cctcgaagtg cttcttgacc cagggattct
     1381 gccccagcgc gtagccggca agcgacatgg aagcgatcca gaggaagcct ccggcgatat
     1441 cgtacggaag gaactttttc ttgtagttca tcctgcctac accggctaca aatggggtga
     1501 acgtgcgtac gatcggaacg aagcgtgcca tgataattgc cttggtcccg tacttttcga
     1561 agaacgtatg cgtgcggtcc atgtactcct gcttgacgag tgctttgccg ccaattttca
     1621 tttttaatgc gcgttgtccg aaaaatctcc cg
//
LOCUS       DAOTON010000703         1651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_57753_length_1651_cov_1.920426, whole genome shotgun sequence.
ACCESSION   DAOTON010000703 DAOTON010000000
VERSION     DAOTON010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57753_length_1651_cov_1.920426"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="P5292_11940"
     CDS             <1..1577
                     /locus_tag="P5292_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS39881.1"
                     /translation="PYSYSWSPGGATTASVTGLNGGQYFVTITDSVGCTTVDSVSISA
                     IPVMGAVVGTQTDVLCPGAATGSATVTLSGGTAPFTYAWTNGDTGSSADSLAAGSISV
                     TVTDANGCVETTSATISEPGAFVFTPTVTDARCSGINDGSVAIGISGGTAPYSVSWSN
                     GDTGLTADSLSGGYVTYTVTDDNGCVVADSANVNQPSSIVSSLSASTDVRCNGDANGT
                     ATATAASGGTAPYSYTWSNGETGLSADSLIAGNTTLTITDANGCTLQLTATIGQPVAF
                     AVTAQGVDVSCFGQTDGSAQAGTTGGISPASFVWIPGGGTTAAITNLTPGTYSVTATD
                     ANGCTSTDVVTIDEPAQLLADAGSDQGGCEPTVSLNAGLGNGQTGTWSVFSGTASFSS
                     ISSPLASVSGLAVGDNLLVWTITDGTCYGYDTLVVRLDGPDLCGLELPSGFSPNGDGK
                     NDGYEIRGVNQYPDNMFRVFNRWGNLVYEREDYTSNDWVGQNKEGDPLPDGTYFVTFE
                     VTGQDIRRSTYVDMRR"
BASE COUNT          353 a          467 c          460 g          371 t
ORIGIN      
        1 ccccgtactc ttatagctgg tcaccgggtg gtgctaccac agcaagtgta acaggcctca
       61 atggtggcca gtacttcgtc acgatcaccg atagtgtcgg atgtacgacc gttgattccg
      121 tatcgatctc cgcgattccc gtcatgggtg cagtagttgg tactcaaacc gacgtattgt
      181 gcccaggtgc cgcgaccggc tccgcaacgg ttacactgag cggtggaacc gcgccgttca
      241 cgtatgcctg gaccaacggt gacacgggca gtagcgccga cagccttgcc gccggaagca
      301 tctccgtaac ggtaaccgat gcgaacggct gcgtggaaac taccagcgca accatttcag
      361 agcccggagc attcgtcttt actccaacgg tcaccgacgc acgatgcagc ggaattaacg
      421 acggttcggt ggctatcggc atctccggtg gtaccgcacc ttacagtgtg agttggtcga
      481 atggtgatac aggtcttacc gctgattccc tctccggtgg atatgtgacg tacacggtca
      541 ccgacgacaa tggctgcgtg gtcgctgatt ccgccaatgt caatcaacca tcatccatcg
      601 tttccagcct gagcgccagc accgatgtac gctgcaacgg tgatgcgaac ggaacggcta
      661 ctgcaactgc cgcctccggt ggtacggctc cttattccta tacctggtcc aacggcgaga
      721 ccggcctttc cgctgacagt ctcattgccg gaaataccac gctcaccatc acggacgcaa
      781 atggttgtac gttgcaactg accgctacga tcggacaacc ggtcgccttc gcggtcaccg
      841 ctcagggtgt tgacgtaagc tgtttcggtc aaacggacgg ttccgcacaa gccggtacaa
      901 ccggcggtat cagcccggca tcgttcgtct ggataccggg cggaggtacg accgctgcta
      961 tcacgaatct tactccgggt acatactcgg taacggcaac ggatgccaac ggttgtacga
     1021 gtacggatgt agttaccatc gacgaacccg cgcaattgct cgctgatgcc ggttcggatc
     1081 agggcggatg cgagccgacc gtcagcctca atgccggctt gggaaatgga caaaccggta
     1141 cctggtcggt gtttagcggc acagcctcct tcagcagcat ttcttcaccg ctggcttctg
     1201 tttccggcct tgcagtcggc gataacctgc ttgtatggac catcaccgat ggtacctgct
     1261 atggatatga tacgctcgtg gtacgactgg atggtcccga tctctgcgga ctcgaactgc
     1321 cctcaggatt ttccccgaat ggtgacggta agaacgacgg ctatgagatc agaggtgtga
     1381 accagtatcc ggataatatg ttccgggtgt tcaatcgctg gggtaatttg gtctatgaac
     1441 gagaagatta cacgagcaac gattgggtag ggcagaacaa ggagggcgac cccttgcctg
     1501 atgggaccta ttttgtgaca ttcgaggtga ccggtcagga tatccgccgc tccacctacg
     1561 tcgatatgag gaggtaaatt cacccgttca ttaaaaatgc gaagggctac tggttacagt
     1621 agcccttcgc atttttatcg tcgtttactc g
//
LOCUS       DAOTON010000704         1647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_57922_length_1647_cov_2.993090, whole genome shotgun sequence.
ACCESSION   DAOTON010000704 DAOTON010000000
VERSION     DAOTON010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57922_length_1647_cov_2.993090"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="P5292_11945"
     CDS             complement(<1..215)
                     /locus_tag="P5292_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRS39882.1"
                     /translation="MAEKITEKDSPYRNLDELPVYDLLAAINKEDQSVPLAIEKILHR
                     IETVVILITEKMRKGGRLFYLGAGTSGR"
     gene            complement(281..>1647)
                     /locus_tag="P5292_11950"
     CDS             complement(281..>1647)
                     /locus_tag="P5292_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRS39883.1"
                     /translation="RRRAWDRFLGPIRLDMQELTTLLEDLAQSSDQSSALLQAAEAMN
                     KSLDPSRRDIATTLHKALRLTRGETSPARTALVQFDQRFRAINADRYNSRLHSTSDEA
                     ATKVEAIAVRYAEDAPLVDGREVLQANFEALFSRDPRLVAFGEDVGKIGDVNQGFAGL
                     QAKFGELRIADMGIREATIAGQGIGLALRGLRPIAEIQYLDYLLYALQILSDDLATLQ
                     YRTKGGQKAPVIIRTRGHRLEGIWHSGSPMGMILHSLRGIHVLTPRNMTQAAGFYNTL
                     LAGDEPGLIVECLNGYRLKERLPSNLGEYRIPLGKVELLEPGSDVTLLTYGSCVRIAQ
                     EAILMLRELGISVELIDAQSLLPFDREHAVVESLRKTNRLVILDEDVPGGASAYLLQQ
                     VLEEQQGFQYLDSAPVTITAKAHRPAYGSDGDYFSKPNAEDVTDSVYRMMHEADPLRF
                     PLYK"
BASE COUNT          355 a          431 c          482 g          379 t
ORIGIN      
        1 cgaccgctgg tcccggcgcc gaggtagaag agtcggccgc cttttcgcat cttttcggtg
       61 atcaggatga ctaccgtctc gatccgatgc aggatctttt cgatggcaag cggaaccgat
      121 tgatcttctt tgttaatggc cgcaagaagg tcatataccg gcaattcgtc gagattgcgg
      181 tagggggaat ctttttctgt gatcttttca gccataaata gtgaaaggcg ttttcttgcg
      241 cccggaattt tcgccgggtc attacaaatg cggtgccaat ttacttataa agcggaaacc
      301 gaagcggatc ggcttcgtgc atcatcctgt acaccgaatc ggtaacgtcc tcagcattcg
      361 gtttggagaa gtaatctcca tcggatccat aggcagggcg gtgagccttc gcggtaatag
      421 tcactggcgc actgtcgagg tactgaaatc cctgctgctc ttccaggact tgttgcagca
      481 gatacgcgct tgcgccaccc ggtacatctt catccaggat aaccaggcgg ttggttttgc
      541 gcagggattc aacaacagca tgctcccggt cgaagggtag gagagactgt gcgtcaatga
      601 gttcgaccga aatgccgagc tcgcgcagca tcaggatggc ttcctgggca atgcgtacac
      661 aggaaccgta ggtaagcaag gtcacatcgg atccgggttc cagtagctcc actttaccta
      721 gcggaatccg gtattcaccc aagttcgaag gaagacgttc tttcaaacgg taaccattga
      781 gacactctac gatcaaaccg ggttcatcac cggcgagtag cgtattgtaa aaccctgctg
      841 cctgggtcat gttacggggg gtcagcacat ggatgccacg cagggaatgt aggatcatgc
      901 ccatggggga gccgctgtgc cagattcctt ccagacgatg gccacgggtg cgtatgatga
      961 ccggggcttt ttgtcctcct ttggtacggt actgcagggt agcaagatcg tcactgagta
     1021 tctgcaaggc gtatagcaaa tagtccaggt attgaatctc cgcgatcgga cgaagtccgc
     1081 gcaaggctag tccgatgccc tgaccggcaa tcgtagcctc gcggataccc atatcggcaa
     1141 tgcgtagttc accgaacttg gcttgtaatc cggcaaatcc ctggttgacg tcgccgatct
     1201 ttccaacgtc ttcaccgaac gcgaccaggc gcgggtctcg ggaaaagagc gcttcgaaat
     1261 tcgcctgcag gacttcccgt ccgtcgacca ggggagcgtc ctccgcatac cggactgcaa
     1321 tggcttcgac tttggtagcc gcttcgtccg atgtgctgtg tagcctggaa ttgtagcgat
     1381 cggcattgat agcacggaac cgttggtcga attgtaccaa agcggtcctg gccggagaag
     1441 tctctccacg ggtaaggcgc agcgccttgt gcagggtagt ggcaatgtcg cgtcgggacg
     1501 gatccagcga cttgttcatt gcctcggcgg cttgtaaaag agcggatgac tggtcgctgg
     1561 actgcgcgag atcttccagc agggtagtta gttcctgcat gtcgagccgg ataggtccga
     1621 ggaaacggtc ccaggcacgc cggcggg
//
LOCUS       DAOTON010000705         1646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_57971_length_1646_cov_3.065996, whole genome shotgun sequence.
ACCESSION   DAOTON010000705 DAOTON010000000
VERSION     DAOTON010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57971_length_1646_cov_3.065996"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="P5292_11955"
     CDS             complement(<1..413)
                     /locus_tag="P5292_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRS39884.1"
                     /translation="MTTDKPLNFLEEIISEDVASGKHGGRVLTRFPPEPNGYLHIGHA
                     KSIYVNFGLAKKFNGQTNLRFDDTNPEKEETEYVESIKRDIQWLGYQWANEFYASDYF
                     GKLYEFAVTLIRKGLAYVDDSTAEEIASMKGTPTEP"
     gene            complement(466..>1646)
                     /gene="gltX"
                     /locus_tag="P5292_11960"
     CDS             complement(466..>1646)
                     /gene="gltX"
                     /locus_tag="P5292_11960"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRS39885.1"
                     /translation="AGGAFSYGCATRLNLKNSLILTQEEVDRRLAAGEPYVIRLKVPE
                     LDTIVLHDLIRGEVRVESNLLDDKVLFKSDGMPTYHLANVVDDYLMQITHVIRGEEWL
                     PSAPLHVLLYRAFGWEDVMPQFAHLPLLLKPDGNGKLSKRDGDRLGFPVFPLNWTDPV
                     TGEVSSGYREKGYYPKAFLNVLALLGWHPSGEQELFSLDELVEAFSLERVSKAGAKFD
                     PEKTKWFNEQYLRKQDDTGIAQRLRLSVHEAYTLDANDPRWSDDYLLQAVALLKDRVQ
                     FESELLPTGRYLFEAPTSYDEAVITKRWKPERTAFFLALCEAFAVLEPFNAETAEQSF
                     QSVAAAQGLKPGEVLQLFRVILSGQGGGVNLFGMVALLGRQQCIDRIQAALVKLQQTA
                     "
BASE COUNT          291 a          472 c          543 g          340 t
ORIGIN      
        1 ggttcggtcg gcgtgccttt catggaagcg atctcctccg cggtgctgtc gtcgacgtag
       61 gccaggcctt tcctgatcaa ggtcaccgcg aactcgtaca gcttgccgaa gtaatcgctc
      121 gcatagaact cgttcgccca ctgatagccg agccactgga tgtcgcgctt gatgctctcg
      181 acgtactcgg tctcttcctt ctcgggattg gtgtcgtcga agcgcagatt ggtctggccg
      241 ttgaatttct tcgcgaggcc gaagttcacg tagatcgact tcgcgtggcc gatgtgcagg
      301 taaccgttcg gctccggggg gaagcgcgtg agtacgcgtc cgccgtgctt gccggaagcg
      361 acgtcttcgc tgatgatttc ttcgaggaaa ttcaggggtt tatcggtggt catgatcggt
      421 agcgtaaagg taggtaggaa tacagcgacg gaagcgcaga acggctcagg ccgtttgctg
      481 cagtttcacg agggcggcct ggatccggtc gatgcattgt tgtcggccca gcagggcgac
      541 catgccgaac aggttcacgc ctccgccttg cccgctcagg atcacccgga aaagctgcag
      601 cacctcgccg ggcttgagtc cctgcgcggc cgccaccgac tgaaaactct gctccgcggt
      661 ttctgcgttg aacggctcca ggacagcgaa ggcctcgcac agcgcgagga agaacgcggt
      721 gcgctcgggt ttccagcgtt tggtgatcac cgcttcgtcg taggaagtgg gcgcttcaaa
      781 gaggtaacga ccggtcggta gcaattccga ttcgaactgc acccggtcct tcagcagggc
      841 gacggcctgc aggaggtagt cgtcggacca gcggggatcg ttcgcgtcga gcgtataggc
      901 ttcgtgcacg ctgagccgca ggcgctgagc gatcccggta tcgtcctgtt tgcggaggta
      961 ttgttcgttg aaccacttcg tcttttccgg atcgaacttg gcaccggcct tgctgacgcg
     1021 ttcgagcgag aacgcctcga cgagttcgtc gagtgaaaac agttcctgtt caccgctcgg
     1081 gtgccagccg aggagcgcga gcacgttgag gaacgccttg gggtagtagc ctttttcgcg
     1141 gtagccggac gacacctcgc ccgtcaccgg atcggtccag ttgagcggga acaccggaaa
     1201 gccgaggcgg tcgccgtcgc gtttgctcag cttgccgttg ccgtcgggtt tcaacagcaa
     1261 gggcaggtgg gcgaactggg gcatcacgtc ttcccagccg aaggcgcggt agagcagcac
     1321 atgcagcgga gccgagggca gccattcctc cccgcggatc acgtgggtga tctgcatgag
     1381 gtaatcgtcc accacattgg cgaggtggta ggtcggcatg ccgtcgctct taaagagcac
     1441 cttatcgtcg agcaggttcg actccacgcg cacctcgcca cggatgaggt cgtgcagcac
     1501 gatggtgtcg agttcgggga ccttgaggcg gatcacatag ggttcgcccg ccgcgaggcg
     1561 acgatcgact tcttcctgcg tcaggatcag ggaattcttc aggttgaggc gggtggcgca
     1621 gccgtaggag aacgcgccgc cggcgg
//
LOCUS       DAOTON010000706         1638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_58381_length_1638_cov_3.629185, whole genome shotgun sequence.
ACCESSION   DAOTON010000706 DAOTON010000000
VERSION     DAOTON010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58381_length_1638_cov_3.629185"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="P5292_11965"
     CDS             complement(<1..1101)
                     /locus_tag="P5292_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39886.1"
                     /translation="MRSVKKSPSRIRFAPRTRQRFRQRLVVASLLLFFLISGVVFVYM
                     NLGDSREAYAVANISWNGNISTDWNTAGNWSPANVPGASDNVTIPNRTNQPVLSANVS
                     VQNLTLNNGSNLKLNGKTLNVTGTFNQQSGGALDLEGGILNASGNAYFSPGTIADAAG
                     TGSVNLTGPTASIGAGGGTLTFAPPLTVNCNAISLRNTVFQSTVDIRKTGGGNDASAG
                     GNTFQAALTCTLQAGGYFVMSNSNGDVYNADVTITNNSSGTFYLSHGGTSTFNGNVTL
                     NNTSTGGFQIGASGGTSNFAADKTFLTSGFSGGPLTMNRVNQSGSSASTFTASLTNFN
                     STNAICIAPISLVCSGTANITNGTFYNDFSLEA"
     gene            complement(1273..1635)
                     /gene="folB"
                     /locus_tag="P5292_11970"
     CDS             complement(1273..1635)
                     /gene="folB"
                     /locus_tag="P5292_11970"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRS39887.1"
                     /translation="MPIFLKNIRSYAYHGCLPEEARIGGNYRVDVRLDFDSGKSERTD
                     DLKDTVDYCQVYDIVQREMDIRSKLIEHVGARIADALLKEIALLDATTVRVTKIAPPM
                     NGDVQEVQFIVRRSRSGK"
BASE COUNT          355 a          446 c          471 g          366 t
ORIGIN      
        1 ggcttccagg gaaaagtcgt tgtagaaggt gccgttggtg atgttcgccg ttccactgca
       61 cacgaggcta atcggcgcga tgcagatcgc gttggtcgag ttgaagttcg tgagggaggc
      121 tgtaaaggtc gaagcgctgc taccgctttg gttcacgcga ttcatcgtca gcggaccgcc
      181 gctgaaaccg ctcgtcagga aggtcttgtc ggccgcgaag ttggaagtgc cgccgctggc
      241 tccgatttga aagcctccgg tgctggtgtt gttgagcgtg acattcccgt taaaggtcga
      301 tgtaccgcca tgcgagaggt aaaaggtacc ggaggagttg ttcgtgatcg tgacatcggc
      361 attgtacacg tcaccgttcg aattggacat cacgaaatat ccgccggcct gcagcgtaca
      421 cgtcagcgcg gcctggaagg tgttgccacc cgcgctggca tcgttaccgc cgccggtctt
      481 gcgaatatcg accgtgcttt gaaaaacggt attccgcagg gagatcgcgt tacaattcac
      541 ggtaagcggc ggagcaaacg tcagtgttcc tcctcccgcc ccaatggagg cggtcggtcc
      601 ggtcaggttg acgctgcctg tgcccgctgc atcggcaatg gttcccggtg agaaatacgc
      661 gttgcccgaa gcattcagga tgccaccttc caggtcgagt gcgccacccg actgctggtt
      721 gaaggttccg gtgacgttca gcgtcttccc gttcagcttc aggttggacc cgttgttcag
      781 ggtaaggttc tgaacgctca cattcgccga caataccggt tggttggtac ggttgggaat
      841 ggtcacgttg tcggatgcgc ccggtacgtt ggctggcgac cagttgccgg ccgtattcca
      901 gtcggtactg atgttgccgt tccaggaaat gttcgcgacc gcgtaggctt cgcgactatc
      961 gcccagattc atgtaaacga aaacgacgcc ggagatcagg aagaacagca acaaactggc
     1021 gacgaccagc cgctggcgga atcgctgacg ggtccgtggc gcgaaccgga tccgggaggg
     1081 tgatttcttg acactgcgca tgtaacttat tacccggacg gcagcgcata cgttgaaaat
     1141 attagaccga tctcttacat gtccggcgag aaaaaatacc gtgccgagtg agaaaatacg
     1201 aaaatttaaa aacatttcct aattcctcat tcccgattcg ggaatcgttc ccaagttgta
     1261 aatgcacgaa cgtcattttc ccgaacggga acgccgcacg ataaactgca cttcctgcac
     1321 atcgccgttc atgggcggag cgatcttggt gacgcgtacc gtagtcgcat caagcagggc
     1381 aatctcttta agcaaagcat ccgcgatcct tgcccccacg tgttcgatca gctttgaacg
     1441 aatgtccatc tcgcgctgca cgatgtcata gacctgacag taatcgacgg tatccttcag
     1501 atcatctgtc cgctccgact tccccgaatc aaaatccaaa cgaacatcca cccggtaatt
     1561 cccgccaatg cgcgcctcct cgggcaggca gccgtggtaa gcgtaggagc ggatgttctt
     1621 gaggaagatt ggcatggt
//
LOCUS       DAOTON010000707         1632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_58707_length_1632_cov_3.585289, whole genome shotgun sequence.
ACCESSION   DAOTON010000707 DAOTON010000000
VERSION     DAOTON010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58707_length_1632_cov_3.585289"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="P5292_11975"
     CDS             <1..555
                     /locus_tag="P5292_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019949437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRS39888.1"
                     /translation="ELIESELFGHEKGAFTSAIKQRIGKFEQASGGTLFLDEIGDMSL
                     SAQAKVLRALQENKITRVGGEKEISVSPRVVAATNKDLKSEIAKGNFREDLYHRLSVI
                     LIHVPSLNERRDDIPMLAEHFLNEICDDYGMARKSFSPEALKELQKLDWTGNIRELRN
                     IVERLVILCDKKITDKDVKQFASR"
     gene            568..1476
                     /locus_tag="P5292_11980"
     CDS             568..1476
                     /locus_tag="P5292_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39889.1"
                     /translation="MAQSIKTVRRFNQLYRNFSFSELMAKADEEFLESVTEMDAKGNI
                     VEESKFDSSGELEERNTYRFGPHGKLEEHVLFYAVDDVTERRVLQRDEKGLLLEEVRY
                     YGDDTGERLVYTYGDKDLLVSLKRYDEEGDFDYEESFSYDANDSLTMRERKRGGGVVV
                     SRMRFSPEGEGTKLEEEEFDEAGKPVSTTVSVYNKDGKEVSSVQRNVQGKLISSVSSV
                     YDEHGNILERKHQDFYSKITRFTYDEQHRLLTQELFDGSGLLLRKNVYEYDNDGRLLT
                     EQNYEIDTSRGGRDKHYGSRYEYEYF"
BASE COUNT          420 a          395 c          467 g          350 t
ORIGIN      
        1 gagttgatcg aaagtgaatt gttcggccat gagaagggag ctttcacttc cgccatcaag
       61 cagcggatcg gtaagttcga acaggcctcc ggcggcacct tgtttctcga cgagatcggc
      121 gatatgagcc tttcagccca ggccaaagtc ctgcgtgcct tgcaggaaaa caagatcacg
      181 cgggtaggcg gagagaagga gatcagcgta agtccgcgcg tcgtcgcggc aaccaacaag
      241 gacctgaaat cggagatcgc caaaggcaat ttccgggaag acctttacca tcgtctgagc
      301 gtcatcctga tccatgtgcc ttccctgaac gaacgccggg atgatatacc gatgctggca
      361 gagcatttcc tgaatgagat ctgcgacgat tacggaatgg cccgtaaatc gttttccccg
      421 gaggccctca aggaacttca aaaactcgac tggaccggta atattcgaga gctgcgcaat
      481 atcgttgagc gccttgtgat cctttgtgat aaaaagataa ccgataagga tgtgaaacag
      541 ttcgcttccc gttgatgcta ctacgctatg gcccaatcga tcaagaccgt ccgccgtttt
      601 aatcagctct atcgcaattt cagtttttcc gaactgatgg cgaaagccga cgaagaattc
      661 ctcgaaagcg taaccgagat ggacgccaag gggaacatcg tggaagagtc caagttcgac
      721 tcgtccggtg aactggaaga acggaacacc taccgcttcg gtcctcatgg aaagctcgag
      781 gagcatgttt tgttctatgc ggttgacgat gtgacagagc ggcgcgtgct tcagcgtgat
      841 gagaagggat tgctgttgga ggaggtgcgt tattatggcg acgatactgg cgaacgattg
      901 gtgtatactt atggcgacaa ggacctgctg gtatcgttaa agcggtacga tgaagaaggc
      961 gatttcgact atgaagaatc gttttcttac gatgccaatg acagcctgac gatgcgggag
     1021 cgcaagcgtg gaggtggggt cgtggtcagc cggatgcgtt tcagtccgga aggagagggt
     1081 acaaagttgg aagaggagga atttgacgag gcaggtaagc ccgtatccac gaccgtatcg
     1141 gtttataaca aggatgggaa agaggtaagt tccgtgcagc gaaacgtgca ggggaaactg
     1201 atttcatccg tcagctcagt ctacgatgag cacgggaaca tcctcgaacg caagcaccag
     1261 gatttctaca gcaagatcac ccgcttcact tatgacgagc agcaccggct cctgacccag
     1321 gagttgtttg atggatcggg actgctcctg cgtaagaacg tttacgagta cgacaacgac
     1381 ggcaggctat tgacggagca gaactacgag atcgacacat cccgtggcgg ccgtgacaag
     1441 cactacggca gccgatacga atacgagtac ttttgattga acggtaacgg gtcaggagat
     1501 caaaccggtc agcttttcca aagcttttac gattccatcc ggctgctttc caccggccgt
     1561 agcgtaaaag gcctggccac cgccaccacc ttgaatctcc ttggcaagtt cccggatcaa
     1621 ggcggaagcg tt
//
LOCUS       DAOTON010000708         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_58977_length_1627_cov_3.220738, whole genome shotgun sequence.
ACCESSION   DAOTON010000708 DAOTON010000000
VERSION     DAOTON010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58977_length_1627_cov_3.220738"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="P5292_11985"
     CDS             <1..783
                     /locus_tag="P5292_11985"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRS39890.1"
                     /translation="QWATIGKEVSHIEEATLQDVRDFFQRYYSPDNAILVVAGGVKAD
                     EVFSLADKWFGTIDRKMGARHPYPVEPVQDTPRELSVNRKVPYDALYMAFHCCSRLDP
                     EFYALDLLTDIMSRGKSSRLYNALVKQRKLFSEVHAYGTGDFDKGLVVVEGKLVKGVK
                     MEDAEAAVWEELNRLTAQPVEATELEKVKNKVESTLEFSEMDLSGRALNLAIAEYMGD
                     PELINTELGLYQAVSAAQVQQQAQQVFNKKNVSVLRYFSERN"
     gene            788..>1627
                     /locus_tag="P5292_11990"
     CDS             788..>1627
                     /locus_tag="P5292_11990"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRS39891.1"
                     /translation="MNLMTSPDRTLAPDFRPITKLDVQKAGQVKLTNGIPVYTINAGF
                     QDLVRIEWLFRLRSFDPQRPLLQNAANRLLQEGTSKKSAQEIADQIDYFGAFLETEEG
                     PDTVSIVLYTLNKHLPKMLPIVRELFADAVYPEHELGIYKQNQVQRLTVENEKVQSLA
                     RRRFLQLLFGQAHPYGFYVSAEHYLQLDREELEAYRRRHYTNDNCTIFVAGKLPADLE
                     KLLDQHFGDSDWKTSESMSESRGVTMLDPTQKHYQEKPGAIQSAIRIGKPLFRRSDPE
                     YPAM"
BASE COUNT          421 a          422 c          416 g          368 t
ORIGIN      
        1 cagtgggcaa ccatcggaaa agaggtgagc catatcgaag aagctacctt acaggatgtt
       61 cgggatttct tccaacgcta ttattcacct gacaatgcca tcctggtcgt tgccggtgga
      121 gtaaaagccg acgaggtttt ttcgcttgct gataaatggt ttggaacgat cgatcggaaa
      181 atgggtgctc ggcatcctta tcctgtcgaa ccggtacagg ataccccgcg tgaattgtcc
      241 gtcaaccgca aagttccgta cgatgctttg tatatggcgt ttcattgttg ctcccgactg
      301 gatccggaat tctatgcgct ggacttgttg accgatatca tgtcgcgtgg taaatcgtcg
      361 cgtctttata atgcactcgt caagcaacgg aagcttttca gcgaggtcca tgcttatggt
      421 accggtgatt tcgataaagg ccttgttgtc gtcgagggca aactggtcaa aggcgtgaag
      481 atggaagatg cggaagccgc cgtttgggaa gaactgaacc gcctgacggc ccagccggta
      541 gaggcaacgg aacttgaaaa ggtaaagaac aaggtggaaa gtacgctcga gttttccgag
      601 atggacctct ccggtcgtgc gctcaacctg gccattgccg agtacatggg ggatccggaa
      661 ctgatcaata cggaactcgg actttatcag gccgtcagcg ctgctcaggt gcagcaacag
      721 gcgcaacagg tattcaacaa gaagaacgta tcggttcttc gctacttctc agaacgaaac
      781 tgatcgtatg aatttgatga cttcacccga ccgcaccctt gcacctgatt ttcgtccgat
      841 caccaaactt gacgtacaga aagccggaca ggttaaattg accaacggca tcccggtata
      901 taccatcaat gccggttttc aggacctggt caggatagag tggttgtttc ggcttcgatc
      961 gttcgaccct caacggccat tactccagaa tgccgccaat cgcctgctcc aggaaggtac
     1021 atcgaagaaa agcgcgcagg agattgcgga ccagatcgat tatttcggag ccttcctcga
     1081 aacggaagaa ggtcccgaca ccgtttcaat cgtgctctac acgctgaaca agcatttgcc
     1141 caagatgctg cccatcgttc gggaactctt cgcagacgcc gtttaccctg aacacgaact
     1201 cggcatctac aagcagaacc aggtgcaacg gctcacggtg gaaaatgaaa aagtacagtc
     1261 actcgcccgt cgccgctttt tacagctctt gttcgggcaa gcacatccat acggattcta
     1321 cgtttcagcc gaacactatc tgcagcttga tcgggaggaa ctggaagcat atcggcgcag
     1381 acactatacc aatgacaact gcaccatctt cgtagccgga aaactgcctg cggacctgga
     1441 aaaattgttg gaccagcatt ttggcgattc cgattggaag acatcggaat caatgtcgga
     1501 aagcagagga gtcaccatgc ttgatccaac tcaaaagcac taccaggaaa aacctggcgc
     1561 cattcagtcg gcgataagga tcggaaaacc cctgttccgt cgctcggatc ctgaataccc
     1621 cgccatg
//
LOCUS       DAOTON010000709         1624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_59137_length_1624_cov_3.444232, whole genome shotgun sequence.
ACCESSION   DAOTON010000709 DAOTON010000000
VERSION     DAOTON010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59137_length_1624_cov_3.444232"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="P5292_11995"
     CDS             complement(<1..667)
                     /locus_tag="P5292_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRS39892.1"
                     /translation="MNALKIYRSSAGSGKTYTLVLEYLHLVLRHPEDYRHILAITFTN
                     KAADEMKSRVIRALTELQQESNPGLAAALQQLLPEVILRDRAALTLKLILHDYSSFSV
                     STIDSFFQRLLRSLAREIHLPLRLEIDLDEEDVCKEVVDRLLREAGKDEELTRWLEQL
                     ILHKIEADRGWNIEQDLFVVAKQLFRDDQGNTRVLDRKTVHDFYHDLTTFRKSFEKSL
                     QEIG"
     gene            784..>1624
                     /locus_tag="P5292_12000"
     CDS             784..>1624
                     /locus_tag="P5292_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRS39893.1"
                     /translation="MIGFRFSKFQPGPEQGSPFDRLLNLFLQLLTYTAGDANEALQWM
                     NELDREHGLTDNEYSMGDFVEDLKRKGYLRDDTNGGLVMTPKAEQSIRKSALEEVFGK
                     LRRSAPGRHSTRFTGIGEEPTGDLREYRFGDTADQISMTESIRNAQINHGLLDFHLME
                     DDLEVSEREHQSQTSTVLMIDISHSMILYGEDRITPAKKVAMALAELIRIRFPKDTLD
                     VIVFGDDAWPISVKDLPYLQVGPYHTNTPAGLELAMDLLRRRKNPNKQIFMITDGKPS
                     CIFE"
BASE COUNT          354 a          443 c          463 g          364 t
ORIGIN      
        1 ggccgatctc ctgcagagat ttttcgaagg atttccgaaa cgtagtcagg tcgtggtaaa
       61 agtcgtgaac agtcttccgg tccagtaccc gggtgttccc ctggtcgtca cgaaagagct
      121 gtttggccac cacgaagagg tcctgttcga tgttccagcc acgatcggct tcgatcttgt
      181 gcaggatcag ttgttcgagc catctcgtca gctcttcatc tttgccggct tcccgcagca
      241 ggcggtccac gacctccttg cagacatcct cttcgtcgag gtcgatctcc agtcgaaggg
      301 gcaggtggat ctcgcgtgcc agggaacgca gcagccgttg aaaaaaactg tcaatggtgc
      361 tgaccgagaa cgaactgtaa tcgtgcagga tcagcttgag tgttagcgcc gctcgatccc
      421 gaaggatgac ttccggcagt agttgctgta atgctgctgc gaggccggga tttgattcct
      481 gctgcagttc ggtcagcgcg cggatgaccc tggatttcat ttcatccgcg gccttattgg
      541 taaaggtgat ggccagaatg tgccggtaat cttccggatg ccgcaaaacg aggtgcaggt
      601 attccagcac cagcgtgtag gtcttgcctg agcctgcgga tgagcggtag atcttcagcg
      661 cgttcacctt tcaaagatag ccgccttgtg ttgtccgtag cggcgttcgc gaactttttc
      721 cgacaaagtt tgtttccagt acagcgtttt ctccccgctc ttggttaact ttggaagtac
      781 agcatgatcg gttttcgctt cagtaaattt caacccggtc cggagcaggg gtcgcccttc
      841 gatcggctcc tgaacctgtt tttgcaattg ctcacctata cggccggtga tgccaacgag
      901 gcgttgcaat ggatgaatga actcgaccgg gagcacgggt tgaccgacaa cgaatattcg
      961 atgggggatt tcgtagaaga cctaaagcgg aaaggctacc tgcgcgacga taccaacggt
     1021 ggactcgtga tgacacccaa agcggagcag tcgatccgca aatccgcctt ggaggaagtg
     1081 ttcggaaagc tcaggcgcag cgcgcccggg cgacattcca cgcgttttac cggaatcggc
     1141 gaagaaccga cgggtgatct gcgggaatac cgcttcggcg atacggccga tcagatcagc
     1201 atgaccgaat cgatccgcaa cgcgcagatc aaccacggac tgctcgactt tcacctgatg
     1261 gaagacgacc tggaggtttc ggagcgggag catcagtcgc agacttcgac cgtcctgatg
     1321 atcgacatca gtcattcgat gatcctgtat ggcgaagacc gtatcacgcc tgccaaaaaa
     1381 gtcgcgatgg cactggcgga gctgatccgg atacgcttcc cgaaggatac gctggacgtc
     1441 atcgttttcg gtgacgatgc ctggccgatc tccgtgaagg acttgcccta cctgcaggtc
     1501 ggcccctatc ataccaatac gcctgccgga ctggaactgg ccatggacct gctgcgccgg
     1561 cggaagaatc cgaacaaaca gatcttcatg atcaccgacg gcaagccaag ctgcatattc
     1621 gaga
//
LOCUS       DAOTON010000710         1619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_59423_length_1619_cov_2.859335, whole genome shotgun sequence.
ACCESSION   DAOTON010000710 DAOTON010000000
VERSION     DAOTON010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59423_length_1619_cov_2.859335"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1619)
                     /locus_tag="P5292_12005"
     CDS             complement(<1..>1619)
                     /locus_tag="P5292_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRS39894.1"
                     /translation="GSASINVTGGQAPFTYQWSPGGGTGVSATGLQAGNYTITVRDRN
                     NCQSSVVVEITERAPLSQNIQSTDIACNGGNTGTATVTASGGTTPYTYSWSNGAGNAA
                     TASLLVAGNYTVTVTDAHGCTSTQSVAITQPPALTASITSNQPVSCYGGSNATTTVTA
                     GGGTAPYDYSWSPSGGMLSTATGLAAGSYTVTVRDANNCIRSVPVTITQPNALLASVG
                     STTNVSCNGGQNGTASTNVSGGTAPYQYVWSPIGTTTANVTGLAAGSYSVSITDARGC
                     QTVTAAIIQEPPLLQTTVLAPVDVSCYGGNDGSATIVATGGTTPYTYNWSAGGSVSTS
                     ANGLPAGSYTVTVTDANACSNTALVVINQPALLSATPTVQNSPLCYGDNSGAASVSVS
                     GGTGPYTYQWSPSGGTDTLASFLPAGIYTVAIRDANGCTSTASATITQPPLLTAAVAT
                     VDATCGASNGSASVTPAGGTAPYTYFWSPGNTTTSSISSVPAGSYSVVITDANGCRLI
                     QSVAVSNIGGPTLANGSVVPVLCAGAMTGAAT"
BASE COUNT          344 a          450 c          479 g          346 t
ORIGIN      
        1 gttgccgcgc ccgtcatggc tccagcgcaa agtaccggca caacacttcc gttggccaaa
       61 gtaggtccgc cgatattcga tacggccacc gattgtatga ggcgacatcc gttcgcgtcg
      121 gtgatgacaa cactgtaact acccgccggc acggaactga tcgaagaagt cgtagtgtta
      181 cccggactcc agaagtaggt gtaaggcgcg gttccccctg caggtgttac ggatgcactg
      241 ccattcgaag cgccgcaggt agcatcgacc gtagcgacag cggcggtcag caatggcggt
      301 tgcgtaatgg tcgcactcgc agtgctggta caaccgttag cgtcgcgaat cgcgacggta
      361 taaattcccg ccgggagaaa cgatgccagt gtatctgttc ctccggatgg cgaccattga
      421 taggtataag gacccgttcc gccgctcacc gataccgatg cagcaccgga attgtcgcca
      481 taacacaatg ggctgttttg cacagtgggt gttgcactta acaacgcggg ttggttaatg
      541 acgacaagtg cggtattgga acaagcattc gcatcggtga ccgtaaccgt gtacgagcct
      601 gcgggtaatc cgttggcaga tgttgaaacc gatccgcctg ctgaccagtt ataggtataa
      661 ggtgtcgttc cgccggttgc aacaatcgta gcactaccgt cattgccacc ataacaagaa
      721 acgtctaccg gcgccaggac ggtcgtttgc aagagcgggg gctcttggat gatggcagcg
      781 gttaccgttt ggcaaccccg cgcatcggta atgctgacgg aataagatcc agccgccagg
      841 ccggttacgt tagccgttgt cgtcccgatc ggtgaccata catactgata aggagcggta
      901 ccgccgctga cattggtcga ggcggttccg ttctggccac cgttacaact cacgttggtg
      961 gtcgagccga ccgacgcaag caaggcgttc ggctgagtaa tggtgacggg gacactgcgt
     1021 atgcaattat tggcatcccg cacggtgacg gtataagaac cggcagcaag accggtagcc
     1081 gtagacagca ttcccccgga aggcgaccaa ctgtaatcat aaggagccgt accgcctccc
     1141 gcagtcaccg tcgtggtggc gttacttcca ccataacacg ataccggttg attggacgtg
     1201 atactggctg tcagtgctgg cggctgggtg atcgcgaccg attgcgttga cgtacaaccg
     1261 tgcgcatccg tgaccgtcac ggtataattg cctgcaacca gaagtgaagc tgttgcggca
     1321 ttgccggctc cgttactcca tgaataagta taaggagttg ttcctccgct agccgtcact
     1381 gttgccgtgc cggtgtttcc tccgttacag gcaatatcgg tgctttggat attttgcgac
     1441 agcggcgccc gctcagtgat ctcgaccacc accgacgact ggcagttgtt gcggtcgcgg
     1501 acggtgatcg tgtaattacc cgcctggagg ccggtggccg aaactccggt cccgccgcca
     1561 ggcgaccatt gataggtaaa cggggcttgt ccgccggtca cgttgatcga tgcggatcc
//
LOCUS       DAOTON010000711         1618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_59482_length_1618_cov_3.182981, whole genome shotgun sequence.
ACCESSION   DAOTON010000711 DAOTON010000000
VERSION     DAOTON010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59482_length_1618_cov_3.182981"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="P5292_12010"
     CDS             <1..1098
                     /locus_tag="P5292_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39895.1"
                     /translation="HFTFRVRTDVPIVAINGSTGASPYLDALFGRDPYFRYRSMPVGQ
                     ADYNALTTPPLVILNQVEAPSSGLLQELERYVRKGGKLMLLPPTAPNREAYQSLAQRF
                     DLPFPGTLVEQATRVGKVETNDPLFRDVFEKGKALPENPDLPLVNGYFNLSAASNRRS
                     DVLMRLENGSPFLQRFPFGSGQVLVLASPLDPQYTSFPQHALFVPVMLKMALSGSSEI
                     DPPVVIGQGAGFSSGDTLLPGEQVYHLVRPSDGFDILPEFRQNESGGTVYVHDQVRQA
                     GTYQLQVNKQLLRPVSFNYDRKESDLRPTEIDEVVRGLQVPADVVDSQSPDLTHAITR
                     AQDGIQLWKYCILLALVFLAIEVALIRLIRT"
     gene            1108..>1618
                     /locus_tag="P5292_12015"
     CDS             1108..>1618
                     /locus_tag="P5292_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRS39896.1"
                     /translation="MELTLSNVCIRLPGSPLNGSQRDIVIADGRITEIAAAGTQRRGE
                     VLTGKGWEVSPGWFDLHVHFREPGDEQKETIQDGLNAAQQGGFSRVLLMPSTNPPIEH
                     PSGLAFAQNRAAQHLVALEMAGCISAGRQGKELAELYDMYQAGARVFTDDQRPVGDTG
                     LMTRALDYVR"
BASE COUNT          348 a          496 c          439 g          335 t
ORIGIN      
        1 cacttcacct tccgtgttcg aacggatgtt cccatcgttg ccatcaacgg cagcacaggc
       61 gcgagtcctt acctggatgc cctgttcggt cgggatcctt atttccggta tcgttcgatg
      121 ccggtcgggc aagccgacta caatgcgctc acgacaccgc ccctggtgat cctgaaccag
      181 gtggaagctc catcttccgg gctccttcag gaactcgagc gttacgtacg taaagggggc
      241 aagctgatgt tgcttccacc gacagctccc aaccgggaag cttatcaatc cctcgcacaa
      301 cggtttgacc tgccttttcc cggcacattg gtagaacaag ccacacgggt cgggaaagtc
      361 gaaacgaacg atccgctctt ccgggatgtc ttcgaaaaag gcaaggcact cccggagaac
      421 ccggacctgc cgctggtcaa tggctatttc aacctaagcg ctgcttccaa ccgtcgcagc
      481 gatgtgctga tgcggctgga gaacggcagt ccatttctgc agcgcttccc attcggatcc
      541 gggcaggtgc tggtgctcgc ttccccactt gatccacaat acaccagttt tcctcaacat
      601 gccttgtttg ttccggtcat gttgaaaatg gcgctcagcg gatcgtcgga gatcgaccca
      661 ccggtcgtga tcggacaagg agcaggcttt tcttccggcg acaccttact tccgggagaa
      721 caggtttatc acctggtccg accatccgac ggtttcgata tcctgccgga attccgacaa
      781 aatgaatcgg gtggtacggt gtacgtccac gatcaggtcc gtcaagccgg cacgtatcaa
      841 ctgcaggtga acaaacagtt attgcgtccg gtctccttca actacgaccg caaggaatcg
      901 gatcttcgtc caaccgagat cgacgaggtc gttcgcggac tgcaagtacc ggccgatgtg
      961 gtcgatagtc aatcgcccga tcttacacac gccatcaccc gtgcccagga cggcatccag
     1021 ctctggaagt actgcatcct gctggcctta gtgttcctgg ccatcgaagt cgctctgatc
     1081 cgtctcattc gaacctaaac ctccgacatg gaactcacgc tcagcaacgt ctgtatccga
     1141 ctaccgggct cgcccctcaa cggaagccaa cgcgatatcg tcatcgccga tggtcgcatc
     1201 acagaaattg cggcagcggg cacccagcga cgcggcgaag tactcaccgg caaaggctgg
     1261 gaagtatcgc ccgggtggtt cgatctgcac gtgcatttcc gggagccggg cgatgagcaa
     1321 aaggaaacca ttcaggacgg tttgaatgct gcgcaacaag gcggctttag ccgggtcttg
     1381 ctgatgccct cgaccaatcc gccgatcgaa catccgagcg ggctggcctt cgcccaaaac
     1441 cgcgcagccc aacacctggt tgcactggaa atggcgggtt gtatctcggc aggacgacaa
     1501 gggaaagaac tcgcggaatt gtacgatatg tatcaggccg gcgcgcgtgt ctttaccgac
     1561 gaccagcgac cggttggcga taccgggctg atgacccgcg cactcgacta cgtgcgca
//
LOCUS       DAOTON010000712         1615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_59662_length_1615_cov_2.978846, whole genome shotgun sequence.
ACCESSION   DAOTON010000712 DAOTON010000000
VERSION     DAOTON010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59662_length_1615_cov_2.978846"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="P5292_12020"
     CDS             complement(<1..824)
                     /locus_tag="P5292_12020"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRS39897.1"
                     /translation="MLVMKFGGTSVAGADRIKALDSIIRDARKPASERTIVVVSAQSG
                     VTDDLIRMSRMAARRDRGYVEVLERVRHRHEETVKLLRLGKDRNLADQLKADFSGLYD
                     TLHGVFLVGELSDRTLDFVSAHGELLSARIIAAYLRSKGRSAGFLDAREVIRTDEQFG
                     NARVNFTTTNRQIGQWLKKHREQVVVMTGFIASTARNETTTLGRGGSDYTAAIAGAAT
                     KAREIQIWTDVDGVLTADPRKVRKAFTVPTMSYREAMEMSHFGAKVIHPPTIQPAL"
     gene            complement(873..1448)
                     /locus_tag="P5292_12025"
     CDS             complement(873..1448)
                     /locus_tag="P5292_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRS39898.1"
                     /translation="MRILLIDNYDSFTFNLVHFLEMASGSLPDVVRNDALDAIDLSRY
                     DAVVLSPGPGLPTESGQLMPFIEKVIGRLPLLGICLGHQAIVEHFGGRLRQLQQVMHG
                     VANTCSILQPPDQLFSGLPDRVQTGHYHSWVADEATWPGCLRITAQDGAQVIQAFAHN
                     ELNVRGLQFHPESVLTPDGYAILCNWVRHLS"
     gene            1511..>1615
                     /locus_tag="P5292_12030"
     CDS             1511..>1615
                     /locus_tag="P5292_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015782777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HRS39899.1"
                     /translation="MKTIIQSEKAPAPIGPYSQAVKAGPFLYVSGQIAL"
BASE COUNT          390 a          453 c          423 g          349 t
ORIGIN      
        1 tccagcgccg gctgaatggt cggcggatgg atgaccttgg caccgaagtg cgacatctcc
       61 atggcttcgc gatacgacat ggtcggtacc gtaaacgcct tgcgtacttt tcgcggatcg
      121 gccgtcagga caccatccac atcggtccag atctggatct cccgtgcctt ggtggctgcg
      181 ccggcaatcg cggccgtata atcggagccg cctcggccga gcgtggtagt ttcattacgc
      241 gccgtcgatg caataaagcc ggtcatgacg accacctgct cacggtgctt cttcaaccat
      301 tgaccgatct gtcggttcgt cgtagtaaag ttcacacgtg cgttaccgaa ctgttcgtcc
      361 gttcggataa cttcccgtgc gtcgagaaaa ccagcggatc gccctttcga acgcaggtac
      421 gctgcgatga tacgggcaga caagagttct ccgtgagcgc tgacgaaatc gagcgtgcga
      481 tccgacagtt ctccaaccag gaagaccccg tgcagtgtat cgtacaatcc ggaaaagtca
      541 gccttcaact gatcagccag atttctatcc tttcccagac gaagtaactt gaccgtttct
      601 tcgtgcctgt gacgcacgcg ctccaatact tccacatatc cacggtcgcg gcgtgcagcc
      661 atcctgctca tccggatcag atcgtcggta acacctgact gcgcagaaac aacaacgatg
      721 gtccgctcgg atgccggttt acgggcatcg cgaatgatcg aatccaaagc tttaatacgg
      781 tcagcacctg cgacagaagt gccgccaaat ttcattacta acatattgat tcttaaaggg
      841 ttctaattaa tacggcattc cccgaaaaat gttcaggaaa gatgccggac ccaattacac
      901 aaaatcgcgt aaccgtcggg ggtcaacacc gactccggat ggaactgtaa accacgcaca
      961 ttcaattcgt tgtgagcgaa tgcctggatc acctgtgccc catcctgcgc tgtgattcgc
     1021 agacaacctg gccaagttgc ttcgtcggca acccaggagt gataatgacc ggtttgaacg
     1081 cgatccggta accctgaaaa aagttgatcc ggcggttgga ggatcgaaca ggtgttggca
     1141 acaccgtgca tgacctgttg caactgacgc aagcgtccgc caaagtgttc cacgattgcc
     1201 tgatgaccca ggcagattcc caacaagggt aatcgtccga tcaccttttc gatgaatggc
     1261 atcaattgac cggactccgt gggtaaaccg gggcccggcg acaagacaac agcatcgtaa
     1321 cgcgaaaggt cgatggcatc cagcgcgtcg tttcgtacaa catccggcag actgccgctg
     1381 gccatttcga ggaaatgaac caggttgaag gtgaacgaat cgtagttgtc gatcagcagg
     1441 atacgcatga gacgttgtcg gaatggagcg gaatgcgttg ctttgaagtg gcaaagtaaa
     1501 gcgacgcgcg atgaaaacca tcattcaaag tgaaaaggct cccgccccta tcgggcccta
     1561 cagtcaggct gtcaaggctg gtcctttcct ctatgtatcc gggcagatcg ccctt
//
LOCUS       DAOTON010000713         1614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_59734_length_1614_cov_2.196280, whole genome shotgun sequence.
ACCESSION   DAOTON010000713 DAOTON010000000
VERSION     DAOTON010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59734_length_1614_cov_2.196280"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1614)
                     /locus_tag="P5292_12035"
     CDS             complement(<1..>1614)
                     /locus_tag="P5292_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRS39900.1"
                     /translation="HLLTEIPTFIGNPMLQVFNSPSTSNGTDGGYSNGYYFYYNPEAI
                     VTVRVDNTTKRYGESLPEFTATILVDGQPLDSTGLSLEDLGLEDMHFLTQATELSPTG
                     FYQVVAEMEPLDLNDGDDIARDTTYEYEFFPGVLKVNKMPITITPRDTTLLFGDKIGG
                     IQFNYDFDQSNVDPAMRPFLIDSISQTHQLTLSNALILVNPRVVTNPRVVTNADFANL
                     AMVVTDKALANPRVVTNPRVVTNPRVVTNATFMVDTMELIDLSAQSVFNYDNDSSVII
                     LETPTAGMRSRAMANPRVVTNPRVVTNPRVVTNGGTEVDSFDVNTSSSDNIAVILDED
                     DVTQPDTSTELKSANLITGTTVGTWLIVPGAFFDDNFAISYGLGSLTIEPAPVTVYAL
                     DTAPDCFVIPAFRDSIMGLQYEDSLHLYDGTPPQFELQDVNGVAVTPTTAGEYQIVPY
                     GYTFPGDSNYVITYVPGTLTLPEPLIVSCVLSGMNCFGNTGTITVTASGGTGPYTGEG
                     IFSVTPGTYSYTVTDALGCTAACTTTVVE"
BASE COUNT          354 a          422 c          483 g          355 t
ORIGIN      
        1 gctcaactac agtcgtggtg caggcagcgg tacaacccag ggcgtctgtg accgtatagg
       61 aataggtacc cggggtgacc gagaagatgc cttcacccgt gtaaggacca gttccaccac
      121 tggctgttac cgtgatagtg ccggtattgc caaagcagtt cataccagac aatacgcagg
      181 atacgatcag cggctcgggc aacgtcagcg ttcctggcac ataggtgatc acataattcg
      241 aatcacccgg gaaggtgtaa ccatatggta cgatctgata ctcaccggcg gtagtcggtg
      301 tgacggcaac gccattcaca tcctgtagct cgaattgcgg cggagttccg tcgtagagat
      361 gcagggaatc ctcgtactga agtcccatga tcgaatcacg gaatgccggt atcacgaaac
      421 aatccggcgc ggtatccagc gcatataccg taacgggcgc cggctcgatg gtcaggctgc
      481 ccagtccgta gctgatggcg aaattgtcat cgaagaacgc tccgggaacg atcagccagg
      541 ttccgactgt cgtaccggtg atgaggttgg ccgacttgag ttccgtgctg gtgtccggtt
      601 gggtcacgtc atcctcgtcg aggatgaccg cgatgttgtc gctcgagctg gtgttcacat
      661 cgaagctatc gacttcggtg ccgccgttgg tcaccacgcg cgggttggtc accacgcgcg
      721 gattggttac cacacgcggg ttcgccatgg cacgactgcg cataccggca gttggcgttt
      781 ccaggatgat gaccgatgaa tcgttgtcgt agttgaacac cgattgagcg gacaggtcga
      841 tcaactccat ggtatccacc atgaaggtgg cgttggtgac cacgcgcggg ttcgtgacga
      901 cacgcgggtt cgtcaccacg cgaggattcg ccagcgcctt gtcggtcacg accatcgcca
      961 ggttggcgaa gtcggcattg gtgaccacac gcgggttggt taccacacgc gggttcacca
     1021 ggatcaaggc gttcgagagt gtaagctggt gcgtctggct gatcgaatcg atcaggaacg
     1081 gacgcatggc cggatcaaca ttgctttggt cgaagtcgta attgaattgg ataccgccaa
     1141 tcttgtcgcc aaacaacagg gtggtgtcgc gcggcgtgat ggtgatcggc atcttattta
     1201 ccttcaagac tcccgggaag aattcatatt cgtaggtggt atcgcgcgcg atatcatcgc
     1261 cgtcgttcag gtccagcggt tccatctcgg caacgacctg gtagaagccg gtaggactga
     1321 gttcggtcgc ctgcgtgagg aagtgcatgt cttccagacc gagatcttcc aacgacaatc
     1381 cggttgaatc cagcggctga ccatcaacaa ggatcgtggc cgtaaactcc ggcagggatt
     1441 caccataccg tttggtcgta ttgtcgacgc gaacggtgac aatcgcttcc ggattgtaat
     1501 agaagtaata cccgttcgaa taaccgccgt cagttccatt ggaggtagag ggcgaattga
     1561 atacctgcaa catcggattg ccgatgaagg tcggaatctc tgtcagcagg tgtt
//
LOCUS       DAOTON010000714         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_59957_length_1610_cov_3.443730, whole genome shotgun sequence.
ACCESSION   DAOTON010000714 DAOTON010000000
VERSION     DAOTON010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59957_length_1610_cov_3.443730"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(360..1531)
                     /locus_tag="P5292_12040"
                     /pseudo
     CDS             complement(360..1531)
                     /locus_tag="P5292_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611566.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          400 a          449 c          309 g          452 t
ORIGIN      
        1 ccgaacctcc tcgattagga aatcgtcgac cagtgaaatc acggaggcga tcccgtactt
       61 cgcaaccttg atgggggtat caatggaaaa gcctgtcccc atgaccggga tatggaagct
      121 gtgtaatttt ttcatagcgg caacattgta atactagtat tagaggatcg caatacttta
      181 ttcataatct gtcaagccaa aatagaaatg tcctagttct accaaaaatg aactacccca
      241 ccctgacggg tgggatagtt cattacctgc ttgatgactt gatacctttg tacctcttct
      301 ctggtcatta tcagaatgtc ctttcgttca tccataacgc ctccttgatc ttccccaatt
      361 cagtggacag tttgtttagt taggacggct cgcttttcaa actcaaaagg acttaagtaa
      421 ttaagcgttg aatgcgtcct cctgcgatta taatacatct ccacgtattc aaagattttt
      481 cttctggctt cttcacgcgt tcgaaaccgg tacttacgaa caagctcgac ctttaacgta
      541 tgaaagaagc tttctgcgac ggcgttatcc caacagtcac ccttccggct catgctgccg
      601 atgaactcat gctcagccag gatcgccttg aaatcatttc cggcgtactg actgccacgg
      661 tccgaatgac agatcaatcc tttatccgga ttacgcctaa ggatcgcctg ctgcatggcg
      721 cgagtcgtca acgtcgcggc aatagtttta tccattgcca gaccaacaat tcctctcgaa
      781 aaaagatcca gcaccactgc aacatataac catccttcag aagtccagat ataggttata
      841 tcaaacaccc agacggcatt gggcttctcg acaataaatt gacgctgaag cacattcggc
      901 cagaccggat agttatgctt tgaatcggtt gttacccgga attttcgctt ctgaacagcg
      961 aagatcccgt taaccttcat caaccgtgct acccggttct ctgaacaacg aatcccctga
     1021 acattcttca attcattcca gatccgcgga ctgccgtaat tgctatcgtt ctccatgaaa
     1081 acacgtctta tctcaatgag caacttgtca ttctcgaccc tttgcctgct tttgggccga
     1141 ttcttgaact tgtaataccc gctccgtgat actcccaatg cccggcacat cttctcgagc
     1201 ggaaagaccg aacagttatc atctataaag atgaaccgtt cccggtcggt tttgaaaata
     1261 tgccgactgc tttttttagg atgtcgcgct ccatctcgac ttcacgcaac tgccgccgta
     1321 acgccgccaa ctctgtcaaa tgacctttac cgggaaaagc cttctccccg tgatcactaa
     1381 actttcgctt ccagctgtaa agctgattcg gatgaatccc caagcttctg gccacctcag
     1441 acgctttatg tccgccttct gcgaccatct tgaccgcgga tatcttgaat tcccggtcaa
     1501 accgcttctt ttttccgttt tccccttcca ttggacacct cccgttttag agccggtcat
     1561 catactaaaa ccaactcttc gttgtgtcca ctttttcggg ggaagatcac
//
LOCUS       DAOTON010000715         1609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_60022_length_1609_cov_3.617117, whole genome shotgun sequence.
ACCESSION   DAOTON010000715 DAOTON010000000
VERSION     DAOTON010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60022_length_1609_cov_3.617117"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="P5292_12045"
     CDS             complement(<1..1285)
                     /locus_tag="P5292_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39901.1"
                     /translation="MKRFFSLLVLLSAWTSLVQAQITFSKEYGGFYDEDGRWMEQTSD
                     SGFVMVGGTETYSNGQSDIWLVRTDAYGNVLWNRSYGDTQFDFANMVRNTSDGGFVIA
                     GFTGSFGAVNNDGWIIKTDGSGNIQWTRMIGDNGIQEIEAIIQTTDGGYAALGYNQST
                     GTQWYDMWLIRLNANGTVRWQKNIGGQGYEIGNSLQQTPDGGFILNGQSYSYGLEDGD
                     YVLYKTDSAGTVQWYKNYDFAGLQESHHVCYTPADGGFLMVGDADDTPNGLGDTDIFL
                     IKTDANGDTLWTKNLGGTKKDGGKTVEVTSDGGYIIGGITRSFGLINPNYYLGKLDAS
                     GNLEWENVTYGSAYHDHAYRAIETSDGGYANFGYFRNASNYQNYALVKLGPGGGVTKD
                     VAIDNYNYPLTSLCRSAGVPISLKLTNYGATNETNI"
BASE COUNT          343 a          405 c          452 g          409 t
ORIGIN      
        1 gaatattcgt ttcattcgta gcgccgtagt tggtcaactt cagggaaatg ggaacaccgg
       61 cgctcctgca cagggaagtc agcgggtaat tgtaattgtc aatggcgaca tcttttgtca
      121 cgccgccgcc cggaccgagt ttcaccagag cgtaattctg ataattcgaa gcgttacgga
      181 agtagccgaa gttggcgtac ccgccgtcgg aagtttcgat ggcacggtaa gcgtgatcgt
      241 gataagcgga accgtaggtt acgttttccc attccagatt gccggaggca tccagtttac
      301 cgaggtaata attcggattg atgagaccga aggagcgggt aattccacca atgatataac
      361 ccccatcgct ggtcacttcg acggtcttgc caccgtcttt tttggtaccg ccgaggtttt
      421 tcgtccagag tgtatcgccg ttcgcgtcgg ttttgatgag gaagatatcg gtgtctccga
      481 gtccgttcgg cgtatcatcg gcatcgccca ccatcaggaa accgccatcg gccggagtat
      541 agcaaacgtg atgggattcc tggagaccgg cgaagtcata gttcttgtac cattgcacgg
      601 taccggcgct gtcggtcttg tagagcacat agtcgccgtc ttcgaggccg tagctgtacg
      661 attgaccatt caggatgaat cctccgtccg gcgtttgttg cagtgagttg ccgatctcgt
      721 agccttgtcc gccgatgttc ttctgccaac gcaccgttcc gttcgcgttg aggcggatga
      781 gccacatgtc gtaccattgt gttccggtgc tttgattata acccagggcg gcataacccc
      841 cgtcggtggt ttgaatgatc gcttcgattt cctgaatgcc gttatcgccg atcatgcgcg
      901 tccattggat gttgccgctg ccgtcggttt tgatgatcca gccgtcgttg ttcacggcgc
      961 cgaaggagcc ggtgaagccc gcgatgacga aaccaccgtc gctggtgttg cgcaccatgt
     1021 tggcgaaatc gaattgcgtg tcgccgtagg accggttcca cagcacattg ccgtacgcgt
     1081 cggtgcgcac cagccagatg tcgctttgtc cgttggaata ggtttccgta cctcctacca
     1141 tcacgaatcc ggaatcggat gtctgttcca tccaacgacc gtcttcatcg tagaagccgc
     1201 cgtactcttt cgagaaggtg atctgcgcct ggacgaggga agtccatgcg ctcaacagca
     1261 caagcaggga gaagaatctt ttcatattga gtctgaggga attcgaatca caaaggtaca
     1321 ggggaagcaa gtccgttctc ggtgttttct gtgaagagtg ttcaacatgg ggtgaatgca
     1381 aatgccgcag cttccagccg gtcgggaccg gtcagaagag aacaaattca ttcgtatgaa
     1441 gttcgggtca gggagggcaa acgggagacc tatttacgcc aaaaccttgc gaatggttca
     1501 ttttttagct gaaaatcggc gccggaatgt gttaaaaatg ggcttttcct ggattttagc
     1561 aagacgggca ttcgtgcgtt gtgtttaacg cctatccgaa cgatgtttc
//
LOCUS       DAOTON010000716         1605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_60275_length_1605_cov_2.627742, whole genome shotgun sequence.
ACCESSION   DAOTON010000716 DAOTON010000000
VERSION     DAOTON010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60275_length_1605_cov_2.627742"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..462)
                     /locus_tag="P5292_12050"
     CDS             complement(58..462)
                     /locus_tag="P5292_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRS39902.1"
                     /translation="MFNFVPRYETSKVERKHRMAIKITEECINCGACEPECPNNAIYE
                     GGAEWRFSDGTSLKGMVKRMDGSEIDANAAQSPVATDIYYIVTDKCTECVGFHDEPQC
                     ASVCPVDCCVDDPDFRESKDELSAKKDRMHAA"
     gene            501..1565
                     /locus_tag="P5292_12055"
     CDS             501..1565
                     /locus_tag="P5292_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HRS39903.1"
                     /translation="MSLQAIEHLGAWFRQTAGNPGDRPELGEEIETRLRAVAVKNPWF
                     SREQVVAAFQAWGQCLQPDAIQRWLNAYPALRQSRSSKVIGVINAGNIPLVGLHDLLT
                     VLITGHAYIGKNASDDSFLLPWMAEKLIEADPALRSRIQFVERLQGMDAVIATGSDNS
                     ARYFEHYFGKYPHIIRKNRNGVAVLTGQETAADLTALGNDIFRFFGLGCRNVSKLYVP
                     ADYQFDDFFSAQVGYADVMSHNKYMNNVDYNNTVFLMKSLPFLSNNFLLLREDPAIAS
                     PIGVLHVERYTSLPTLASQFSEHPEKLQCIVCSEESERALKKETNVMLPYVRFGNSQL
                     PALWDYADGVDTVNFLLGIR"
BASE COUNT          385 a          412 c          410 g          398 t
ORIGIN      
        1 ataagaaccg cggtacgaga acgcaccgcg gttactttta ttcgatcaac agtcggatca
       61 tgcagcgtgc atgcgatcct tcttggctga aagttcatct ttcgattcgc ggaagtcagg
      121 atcatcaaca caacagtcga cgggacaaac cgaagcgcac tgcggctcgt catggaagcc
      181 gacacactcc gtgcacttat ccgtaacgat gtagtaaata tccgtggcaa ccggagattg
      241 ggcggcgttc gcatcgatct cggaaccgtc catgcgtttc accataccct tcagcgaggt
      301 gccgtcggag aagcgccact cagctccgcc ttcgtaaatg gcgttgttcg gacattcggg
      361 ttcgcaggcg ccgcagttga tgcactcttc ggtaatcttg atggccatac ggtgtttcct
      421 ttctactttt gaggtttcgt aacggggaac aaaattaaac agaatttctt cccttcaaaa
      481 ttttgcaaaa atcagacact atgtccctcc aagccatcga acatttaggt gcctggtttc
      541 gccaaacggc tggaaatccg ggggataggc cagagttggg agaggagatc gaaacccggc
      601 tgcgagcggt cgctgtcaag aatccctggt tcagccggga acaggtggtt gcggcttttc
      661 aggcctgggg acaatgtctg caacccgatg ccattcagag gtggttaaac gcttacccgg
      721 ctttacgaca atcgcggtcc tccaaagtga ttggtgtcat caatgcgggc aatatcccat
      781 tggtgggatt gcacgacctt ctgacggtct tgatcaccgg tcatgcctat atcggaaaga
      841 atgcttccga tgattcattc cttttaccct ggatggccga gaagttgatc gaggcggacc
      901 ctgctttgcg atcccgcatt caatttgtcg aaaggctcca gggtatggac gctgtcattg
      961 ccaccggtag tgacaactcc gcgcgttact tcgagcatta ctttggaaaa tacccgcaca
     1021 tcattcgcaa gaaccggaac ggtgtagcag ttctgaccgg acaggagacc gcagcggacc
     1081 tgacggcgct gggaaacgat atctttcgtt ttttcggcct gggttgccgg aacgtatcaa
     1141 aactctacgt gccggctgac tatcaattcg atgatttctt ttcggctcag gttggctatg
     1201 ccgatgtcat gagtcataac aagtacatga acaacgtgga ctacaacaac acggtgttcc
     1261 tcatgaaaag cttgcctttc ctttcaaaca acttcttatt gctcagggaa gatccggcca
     1321 tcgcttctcc gatcggcgta ctgcatgttg aacggtacac atcgcttccg accctggcct
     1381 cccaattctc agagcatcca gaaaaactac aatgcatcgt ctgttcggag gaatcggaaa
     1441 gagcgttgaa gaaagaaaca aatgtcatgc tgccttatgt ccgcttcgga aattcgcagc
     1501 tcccagcctt atgggactat gcggatggcg ttgatacggt gaactttctg cttggtataa
     1561 gataaatttg gcggaggagg cagtatcagg aatcccatct tctac
//
LOCUS       DAOTON010000717         1600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_60552_length_1600_cov_2.772168, whole genome shotgun sequence.
ACCESSION   DAOTON010000717 DAOTON010000000
VERSION     DAOTON010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60552_length_1600_cov_2.772168"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="P5292_12060"
     CDS             complement(<1..602)
                     /locus_tag="P5292_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HRS39904.1"
                     /translation="MTKEPIIDLRSDTVTKPTPGMLEAMFSAEVGDDVFGEDPTVRRL
                     EERSARIFGKEAALFCPSGTMANQIALRLHTELQDEVICDKLAHIYYYEGGGPAANSG
                     VSLRMVEGDRGRLTPELVRQNINDPDNYHLPRTRLVWLENTSNKGGGSYYDVNGMRAV
                     TDLARSYGLSTHLDGARIFHALTESGDQPQAVGACFDSVT"
     gene            complement(680..1087)
                     /locus_tag="P5292_12065"
     CDS             complement(680..1087)
                     /locus_tag="P5292_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39905.1"
                     /translation="MKNRTILLLAGALFIATSAFSQQGGPRNEMTKEQRAEHSSQRMK
                     AQLGLTDEQTQKIYDVMLKREEQLVNNRDAMRQRREEMDKQLIQILTPEQFDKYQQNR
                     MERREKMKDRRRGQQGPPASKMQEPAPVTPEKN"
     gene            complement(1131..>1600)
                     /locus_tag="P5292_12070"
     CDS             complement(1131..>1600)
                     /locus_tag="P5292_12070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS39906.1"
                     /translation="RILEIGTYTGYSALCLAEGLADGGVLETIDINDELTPMVRRYIQ
                     RAGFEDRIRLHCGNAMEVIPQIEGMFDLVFIDADKRNYPNYYRLVRERMQPGGWILAD
                     NVLWSGKIVQPQEKMDADTRALAAFNDAVHADPQADHFLAPIRDGILIIRMRN"
BASE COUNT          317 a          450 c          430 g          403 t
ORIGIN      
        1 aaggtgacgc tgtcgaagca tgcgccgaca gcctgtggct gatcaccact ctccgtgagc
       61 gcatggaaga tccgtgctcc gtcaagatgc gtgcttaaac cgtaactcct ggccaagtcg
      121 gtgaccgcgc gcatcccgtt cacatcgtaa taactgccac cgcctttgtt gctggtattc
      181 tccagccaca ccagacgtgt acgcggcaaa tggtagttgt ccgggtcatt gatattctgc
      241 cgaaccaatt caggcgtaag tcggccacgg tcaccctcga ccattcgaag cgatactccg
      301 gaattggccg ccggacctcc tccttcgtag taataaatgt gggcaagttt gtcgcagatc
      361 acttcgtcct gcaattcggt gtggagccga agtgcgatct ggttcgccat cgtacccgat
      421 ggacaaaaca gggcggcttc tttgccaaag attctggccg atcgttcctc caggcggcgg
      481 acggtcgggt cttctccgaa tacatcgtcg ccgacttcag cggagaacat ggcctccagc
      541 atgccgggtg tcggtttggt gacggtgtca ctgcgcagat cgatgatcgg ttctttcgtc
      601 ataggagggt cttgtatgac gaagatacag ctagcctggc aacccgaaat aaaaagcccc
      661 gctaaagcgg ggctggaaac tagttctttt ctggtgtaac tggagccggt tcctgcatct
      721 tgcttgccgg tggaccctgc tggccacggc gacggtcttt catcttctcc cgacgctcca
      781 tcctgttttg ctgatatttg tcgaattgct cgggcgtcag gatctggatg agttgtttgt
      841 ccatttcttc ccgccgctgg cgcatggcat cccggttgtt gaccaactgc tcttcccgtt
      901 tcagcatcac atcgtagatc ttctgagttt gctcgtcggt caggcctaac tgcgctttca
      961 tccgttgtga tgaatgttcg gcgcgttgct ctttggtcat ctcgttgcgc gggccgcctt
     1021 gttgtgaaaa ggcagaggtc gcgatgaata atgcgccggc aagcagcagg atcgtccggt
     1081 ttttcatggt cgttggaatt gcgggtttga cgaagataat atccttcggt ttaattccgc
     1141 atgcgaataa tcaggattcc atcccggatc ggggctagaa aatgatcggc ctgcggatct
     1201 gcatgcacgg catcgttgaa tgctgccagc gcccgggtgt cggcatccat cttttcctga
     1261 ggttgaacaa ttttaccact ccacaggaca ttgtcagcca ggatccaacc acccggttgc
     1321 atgcgttcgc gcaccagtcg ataatagttc ggataattcc gtttgtccgc atcaatgaac
     1381 accagatcga acattccctc gatctgcgga atgacttcca tcgcgttgcc gcaatggagc
     1441 cggatgcggt cttcgaatcc ggcccgctga atatagcgac ggaccatggg tgtcaattca
     1501 tcgttgatgt cgatcgtctc gagcactcct ccatccgcca agccctccgc cagacaaagc
     1561 gccgaataac cggtgtacgt gccgatctcc aggatacggc
//
LOCUS       DAOTON010000718         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_60621_length_1599_cov_2.542746, whole genome shotgun sequence.
ACCESSION   DAOTON010000718 DAOTON010000000
VERSION     DAOTON010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60621_length_1599_cov_2.542746"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="P5292_12075"
     CDS             <1..1426
                     /locus_tag="P5292_12075"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39907.1"
                     /translation="RTGATLQTIPSAVHGLAGIYGLAFDAVTSGGPYLWAFARITPDG
                     LHRIVRIPVASGIPDGVSFPVMHRLGDQLAYGQAGGLSIANGLAPGRLSLVGVLQGVP
                     DDLLFSVELSSDSLLAADAAMDTLRLSPPYTRVPLALAQAYELEWNCKFRNVGTDTLD
                     VVEVQAALYDSLQSLLVNTTRSVAAMAPADRSSGYFPTGFHPSATGRIRVVASLLSTP
                     ADQFTPNDSLDFRFEVSDSTLAYDEGPAFTGLGLGNGGRVGQSFELPVGAEISSITFS
                     LEAPVAGDSLYAELWTFNGAPGNLIVRTLTYHVPPAYVAGTPVTLPLESGLFPLNDGE
                     YFVCLREHTNSASLSSNWKNYRYRKAWAKSSFGSWQPMEASDLFVGFRLQVNIRQIVN
                     ALNSIAPTSGIRVFPNPTQRDLRVISDQPLQGRLLITDLQGRRLRESSATGEHDLHLS
                     LDGLASGVYLLQTEAGNCRILKTD"
BASE COUNT          299 a          470 c          462 g          368 t
ORIGIN      
        1 ccggactggc gcgaccttgc agactattcc gtctgccgtg cacggactgg ccggcatcta
       61 cggccttgca ttcgacgcgg tgacgtccgg tgggccatac ctctgggcct ttgcacggat
      121 cacgccggac ggtctccacc ggatcgtacg cataccggtc gcgagcggta ttcccgatgg
      181 tgtctctttc cccgtcatgc accgactggg cgatcagctc gcgtatggtc aggcaggagg
      241 cttgtccatt gccaacgggc tcgcacccgg ccgcctgagt ctggtcggag tactgcaggg
      301 agttcccgac gatctgctct tctcggtcga actcagctcg gattcactgc tcgcagcgga
      361 tgccgcgatg gataccctgc ggctctcgcc accctatacg cgtgtgccgc tggcgctggc
      421 gcaggcttat gaactggaat ggaactgtaa gttccgaaat gtcggtacgg ataccctcga
      481 tgtggtcgaa gtgcaagctg ccctctacga ttcgcttcag tccctgctcg tgaacacgac
      541 acgaagtgtt gcagcgatgg cgcctgccga tcgatcttcc gggtactttc ctacagggtt
      601 ccatccttcg gccacaggcc gcatccgggt agtcgccagt ctcctcagta cacccgcgga
      661 tcagttcacg ccaaacgatt cgctcgactt tcgattcgag gtaagtgatt ccaccctggc
      721 ctatgatgaa ggaccggctt ttacaggtct cggactcggc aatggtggtc gagtcggaca
      781 atccttcgag ttgccggtcg gggcggagat ctcttccatc accttttcac tcgaagcacc
      841 tgttgcaggt gatagcttgt atgctgagct ctggacattc aacggtgcac ccggcaacct
      901 gatcgtccgc acgcttacct atcacgtgcc cccggcttat gttgcgggca cgccggttac
      961 cttgccgttg gaatcgggct tattcccctt gaatgatggc gagtatttcg tttgtctgcg
     1021 cgagcacaca aacagcgctt cgctgtcttc gaactggaag aactaccgtt accgcaaggc
     1081 ctgggcgaag agttccttcg ggtcctggca gccgatggaa gcttcggacc tgttcgtcgg
     1141 tttccgttta caggtcaaca tccggcagat cgtcaatgcc ctgaattcaa tcgcgccgac
     1201 ttccggtatc cgtgttttcc cgaacccgac tcagcgagat cttcgagtga tctcggacca
     1261 accgcttcag ggccggctgt tgatcaccga cctgcaagga cgacggctac gtgaaagttc
     1321 ggctaccgga gaacacgatc ttcacctgag tcttgacgga cttgccagcg gagtttactt
     1381 gttgcagaca gaagcaggta attgccgcat cctgaaaacg gactgagccg ggcttcaggc
     1441 gggaaccgcc ttcagctgca gcagccggtg gatggaaaac gaaacgtcca tgaagaggat
     1501 cttcgcgttg gcgttgcgtt cgaggtggaa gatggcgtcg ttgagggttt ttgaaaagat
     1561 gtccatgttg ccggtatgca cgaaaggcgc gaacttccg
//
LOCUS       DAOTON010000719         1595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_60817_length_1595_cov_3.616234, whole genome shotgun sequence.
ACCESSION   DAOTON010000719 DAOTON010000000
VERSION     DAOTON010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60817_length_1595_cov_3.616234"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1561)
                     /gene="porU"
                     /locus_tag="P5292_12080"
     CDS             complement(<1..1561)
                     /gene="porU"
                     /locus_tag="P5292_12080"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HRS39908.1"
                     /translation="MTYFNNVGTSPQDNYALPISFNQAFLPSSSTISASISFSSSDPN
                     ALGWVNYLEMNARCQLTATGRGTQMGFRDVRSVGTGNITEFNLTSVTNSHQVWDITDP
                     LNVAIQDHRFLGNQILFTTETSVLKEFQLLDGVNYLVPVKAGGIANQNLHALPQAGMI
                     IVAPEGFLSTADKLADYHRSHDGLVVHVVTHRQIFNEFSSGAQDVSAIRDFMKMFYDR
                     ATSPADMPRYLLLYGDASYDMKYRITNNSNIVTSYQSAGSLNQTQTYMSDDFFGLLDD
                     TEGDWTSGEIVDMAVGRLPVRTATEAEAMLNKILRYGGGDDPAIVLGDWRNTITFVGD
                     DQDSNTHFKQSDTLAVRNDRNEPVYNIDKIYLDAYNQISTPGGQRYPDAQQAIIDRVQ
                     RGTLLLTYIGHGGEVGWAHERILEVSDINGWTNFNKQAAFLTATCEFTRVDDPSRTSA
                     GELVMLNPNGGGICLFTTSRLAFSSSNNNLCQRFFTHVFTPINGRMPTMGEVFEQTKI
                     DVYTDPYVRNFL"
BASE COUNT          353 a          404 c          481 g          357 t
ORIGIN      
        1 ggaggaagtt gcgcacataa ggatccgtgt aaacgtcgat cttggtttgc tcaaacactt
       61 cgcccatcgt cggcatgcgt ccgttgatcg gcgtgaatac atgggtaaag aatcgctggc
      121 acaggttgtt gttggaactg gagaaggcga gccgggaggt cgtgaacaga caaatgccgc
      181 cgccgttcgg gttcagcatg accaattcgc cggctgaggt gcgcgacgga tcgtcgaccc
      241 tggtgaattc gcaggtcgca gtaaggaagg cggcctgctt gttgaagttg gtccacccgt
      301 tgatatcgga aacttccagg atgcgttcgt gcgcccagcc gacttcacca ccatgaccga
      361 tataggtcag caacagcgtg ccgcgttgca cccggtcaat gatcgcctgc tgggcatccg
      421 gataacgctg acctcccggt gtcgagatct ggttgtaggc gtccagataa atcttgtcga
      481 tgttgtaaac cggttcgttg cgatcgttac gaacggcgag ggtatccgac tgcttgaaat
      541 gcgtattgct gtcctggtcg tccccgacaa aggtgatcgt gttgcgccag tcgccaagga
      601 ctattgccgg atcgtcgcca ccgccatacc ggaggatctt gttcagcatc gcttccgctt
      661 ccgtcgcggt acgcacgggc aacctgccca cggccatgtc aacgatctcg ccgctggtcc
      721 agtctccttc cgtgtcatcc agaagaccga agaagtcatc cgacatgtac gtctgggtct
      781 ggttgagcga accggccgac tggtaactcg taacgatgtt gctgttgttg gtgatccggt
      841 acttcatgtc gtacgaagcg tctccgtata ataagagata ccggggcatg tcagccggac
      901 tcgttgcgcg gtcataaaac atcttcatga agtcacggat cgcggatacg tcctgtgctc
      961 cggaggaaaa ctcgttgaag atctgacgat gggttacgac atggacaacc aatccgtcgt
     1021 gggatcggtg ataatcggcg agtttgtcgg ctgtcgacaa gaacccttcc ggagcgacga
     1081 tgatcatgcc ggcctgaggc aaggcatgca ggttctggtt cgcgataccg cctgccttga
     1141 cgggtaccag gtaattgacg ccgtccagca actggaattc cttcaggacg gatgtttcgg
     1201 tagtgaagag aatctgatta cccagaaaac ggtgatcctg gatcgcgaca ttgagcggat
     1261 ccgtaatgtc ccagacctgg tgagaattcg tgacagatgt gaggttgaat tcagtgatgt
     1321 tgccggtgcc gacagaccgg acatcccgga agcccatttg cgttccacga ccggttgccg
     1381 tgagttggca gcgggcgttc atctccagat agttcaccca accgagcgca ttggggtccg
     1441 aagagctgaa ggagatggaa gcggagatag tagaggagga gggaaggaaa gcttgattga
     1501 aggagatcgg caatgcgtaa ttatcctgcg gactggtgcc cacgttattg aagtacgtca
     1561 caattggact tatcccggat agcgaagcgg atacc
//
LOCUS       DAOTON010000720         1592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_61006_length_1592_cov_2.849707, whole genome shotgun sequence.
ACCESSION   DAOTON010000720 DAOTON010000000
VERSION     DAOTON010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61006_length_1592_cov_2.849707"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="P5292_12085"
     CDS             complement(<1..684)
                     /locus_tag="P5292_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRS39909.1"
                     /translation="MEKEQLPPSTPAPKRKLLPVILTLVVVSIAAWGYSKFTYARHHE
                     DTDDAQIDGNISPVIGRVSGYVNEVKFEDNQFVNKGDTLLLIDDRDLRIKVQQAEAAL
                     ENAEAGVLVAQANVTTAEANLATAQANVDAARVRVWKTGQDYKRYEALMRDQVGTKQQ
                     FDAVKAEKESAEAQQTAVRRQAETASMQVVAAKQQVAVAASLVGQRRTDLDYARLQLS
                     YTVILAPAKG"
     gene            complement(697..1323)
                     /locus_tag="P5292_12090"
     CDS             complement(697..1323)
                     /locus_tag="P5292_12090"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRS39910.1"
                     /translation="MSQTKREQILDVSERLFSEKGFEGTSVRLIASEAGVNVAMISYY
                     FGSKEKLFESLVERKVLLMRERLENLSLEIADPLERMLAIADLYLGKMIAQRPFHRIV
                     FRELTLQQRGDAHETIAESIYGNARMVMDTLREGIHTGRFRQVDVEVTFLNFIGMLQF
                     IVNGSQPLIERIVGATFSESEDALTVLRERLRAHLHDFIRHYLLPEKE"
BASE COUNT          378 a          416 c          387 g          411 t
ORIGIN      
        1 tccttttgca ggcgcaagaa tgaccgtata agaaagttgt aatcgggcat aatccagatc
       61 ggtacgccgt tggccgacca atgacgcggc aacggcaacc tgctgctttg cggcaacgac
      121 ctgcatggag gccgtttcgg cctgacggcg taccgcggtc tgttgcgctt cggcactttc
      181 cttttcagcc ttcactgcat cgaactgttg tttcgtcccg acctgatcgc gcatcaaggc
      241 ttcgtatcgt ttatagtcct gacccgtttt ccagacacgc acccgtgcgg catcgacatt
      301 cgcttgggct gtggccaggt tggcttcggc ggttgttaca ttcgcctgtg caaccaatac
      361 cccggcttcg gcattctcca gtgcagcctc ggcttgctga acctttatgc gcaggtcacg
      421 gtcgtcgatg agcaggagcg tatcgccctt gtttacgaat tgattgtcct cgaacttcac
      481 ctcattgaca tacccggaga cacgaccgat cacgggactt atgttaccgt ctatttgggc
      541 atcgtcggtg tcttcgtgat ggcgagcata ggtgaacttg gaatatcccc atgccgcgat
      601 cgacacaact accagggtca ggataaccgg aagtagcttg cgtttcggag caggagtcga
      661 aggagggagt tgttcttttt ccattggtgc aacgaactat tctttttcag gcaacagata
      721 atgtcggata aaatcgtgga ggtgggcgcg caagcgttcg cggagaacgg tcaatgcatc
      781 ctccgattcg gagaaggtgg caccaacgat gcgttcgatc aacggctgag aaccgttgac
      841 gatgaattga agcatgccga tgaaattcag gaacgtcact tccacatcca cctggcggaa
      901 ccgaccggtg tggatacctt cccgcaaggt gtccatgacc atccgggcat tcccatagat
      961 gctttcggcg atcgtttcat gggcatcgcc gcgttgctgc aacgtgagtt cgcggaatac
     1021 aatgcggtga aagggacgtt gcgcgatcat ctttccgagg tagagatccg cgatcgccaa
     1081 cattcgttcc agcggatcag cgatttccaa gctcaggttt tcaagtcgct cgcgcattag
     1141 cagcacttta cgttctacca gcgattcgaa cagcttttcc ttcgagccga agtagtagga
     1201 aatcatcgcc acattgacgc ccgcttcaga cgcgatgagt cggacgctgg tgccttcaaa
     1261 ccccttttca gaaaaaagtc gctcggacac atccaggatc tgctctcgct tagtttgact
     1321 catcgggccg ctaaattaaa cagttgttta atgttaaaca atcgtttaat attaatttgc
     1381 caaaaatttc gaaaaatcat ctttctacat ctatctatag acatttattt aatcattaat
     1441 caaaatgatt aaataatagc atgaattgac cgatcaggca agaccagccg ccttatacct
     1501 ctatctatac aatccattca aagcccattt aggtcttaaa aagcctaact ttgcgaccga
     1561 tttcgctgtt aaatgcctga aatccggagc at
//
LOCUS       DAOTON010000721         1592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_61046_length_1592_cov_1.642160, whole genome shotgun sequence.
ACCESSION   DAOTON010000721 DAOTON010000000
VERSION     DAOTON010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61046_length_1592_cov_1.642160"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1592)
                     /locus_tag="P5292_12095"
     CDS             complement(<1..>1592)
                     /locus_tag="P5292_12095"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HRS39911.1"
                     /translation="RSSFDSSCASGFGNSDGTSIYDTNLLNSASGRVDGYLALDQPGA
                     GTGPIGAQTSDPIYIWGNTSGKICLGGTNQYASCTSNSDCPSSTCSTKVTTPNNVYLH
                     NNINNQPYHIVSGRDYFENTARPGYTKYTCPHPLAGSGSCDSTVAGRAGYAITEPSVT
                     VPAPNPVATYAVTVSKNGSGTVTSSPLGIDCESTCSYSFTSGTSVTLTAVADTGYSFG
                     SWSGACTGTNATCTLTMDAAKSVTATFIQNTHPLTVTKAATNTGTGIVTSADSGITCG
                     SDCSESYTYGTIVTLSAAADSGSAFYGWSGACTGTTSTCQVTMDAAKNVIASFLAQGS
                     PALITSKKGTGNGKIKSISAVLASNIAASNSIDCGDVCVAQYDNATKVTLTAEPEAGS
                     TFSGWEGACSGTGACVVTVNSLTSVVATFSLQASGGSPTTMLSVGGDGGGGGGGCFIA
                     TAAYGSYLDPHVMTLREFRDKVLLRNSFGRVFVKFYYAHSPAIAHAISQNDVLRVSTR
                     LLLTPIILTVIYPYTAGMIFLT"
BASE COUNT          372 a          397 c          439 g          384 t
ORIGIN      
        1 ccgtcaaaaa aatcatacct gccgtatagg ggtagattac cgttaagata atcggcgtca
       61 ggagcaggcg ggtcgagact ctcaaaacgt cattctgaga gatcgcatgc gctatcgccg
      121 gagaatgtgc ataatagaat ttcacgaaca cgcggccaaa ggaattcctt aacagaactt
      181 tgtcacggaa ttcccgtagt gtcataacgt gtgggtcaag gtaagaacca tacgccgctg
      241 tagcgatgaa gcaccccccg ccgcctccgc ctccatcacc gccgacactg agcattgtcg
      301 tcggtgaacc gccgcttgcc tgcagcgaga atgtcgcgac aaccgaggtg agtgagttga
      361 cggtgacaac acaagcaccc gttccggagc aggcgccttc ccaaccggaa aacgtgctgc
      421 cggcctctgg ttccgcggtc agggtgacct tcgtggcgtt atcatactgg gcgacgcaga
      481 catcaccgca atcaatgcta ttcgaagcgg ctatattgga tgccaacaca gccgatatgc
      541 ttttaatctt gccgttgccg gtcccctttt tgctggtgat gagggcaggg ctgccttgcg
      601 ctaggaaaga ggcgatgacg ttttttgcgg catccatcgt tacctggcag gtcgatgttg
      661 ttcctgtaca ggcgccggac cagccataga aggcgcttcc cgaatccgca gcggcggaca
      721 aagtgacgat ggtcccatag gtataagact ctgaacagtc gctgccgcac gtgatccctg
      781 aatcagcgct ggtgacgatg cccgtaccgg tattcgttgc ggccttggtg acggttaatg
      841 gatgggtgtt ttgaatgaaa gtggccgtta cggactttgc cgcatccatt gtaagagtgc
      901 atgttgcatt ggtgcctgta caggcaccgg accagctgcc gaaagaataa ccggtgtcgg
      961 caacggctgt cagcgttacg gacgttcctg aagtgaagct gtaagagcaa gtggattcgc
     1021 aatcaattcc caaaggtgaa ctggtgactg tgccgctgcc gtttttagag acagtcactg
     1081 catacgttgc gactggatta ggggcgggaa ccgttacgga aggttccgta atggcgtagc
     1141 cagctctgcc agccaccgtt gagtcacatg aacctgaacc ggccagcggg tgagggcatg
     1201 tatattttgt gtagccaggc cgtgctgtgt tttcgaaata atcacgtccc gatacgatat
     1261 ggtatggttg gttattaatg ttgttatgca gataaacatt attaggcgta gttactttgg
     1321 tgctgcaggt gctactcgga caatcactat tggatgtaca ggatgcatat tgatttgtac
     1381 cacccaggca tattttccct gaagtattcc cccaaatata aataggatct gatgtttgag
     1441 cgccaatggg acctgtgccg gctcccggtt ggtccaatgc caagtaacca tcaactcttc
     1501 cactggcaga atttaataaa ttcgtgtcgt atatgctggt gccgtcgcta ttaccgaatc
     1561 ctgaggcaca ggaactgtcg aagctacttc tg
//
LOCUS       DAOTON010000722         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_61092_length_1591_cov_2.348958, whole genome shotgun sequence.
ACCESSION   DAOTON010000722 DAOTON010000000
VERSION     DAOTON010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61092_length_1591_cov_2.348958"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="P5292_12100"
     CDS             complement(<1..421)
                     /locus_tag="P5292_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39912.1"
                     /translation="MHCALLSTAQQLPAIVRDWAAYAGQTDSVFKTPVVLDEDSNLYV
                     GTFRVDSVTGADVLIVKYGTDGGVKWEVSWSGAGLGRDQVSDLAYYNGAVYVSGITQG
                     SASNNYDMLFMRVSSEGIVEWVNTWNGPASSFDVGTAV"
     gene            962..>1591
                     /locus_tag="P5292_12105"
     CDS             962..>1591
                     /locus_tag="P5292_12105"
                     /inference="COORDINATES: protein motif:HMM:NF037833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-type lectin-domain containing protein"
                     /protein_id="HRS39913.1"
                     /translation="MRRLLLCSLGVLLFLAAIPVHGQFQRNGTAVNTSCDCYRLTLSA
                     GGQNGSIWNQQRIDLGNSFDYNFRVNFGCSDAGADGVVFALQTNPLATGVSGGGIGYQ
                     TISPSVAVEFDTYQNGWDPTYDHIAIVSNGDVDHTTGNNLAGPVAALASSGNIEDCLF
                     HDVRIRWNAGSMTLEAYLDGALRLSYTGNLVANIFGGNAQVYWGFTSSTG"
BASE COUNT          371 a          443 c          415 g          362 t
ORIGIN      
        1 gcaccgccgt tcccacgtcg aaggaagagg ccgggccgtt ccaggtattg acccactcga
       61 cgataccctc cgaactaacc ctcatgaaga gcatatcgta gttgtttgag gcgctcccct
      121 gggttattcc ggaaacgtac acggctccgt tgtaataggc caggtcgctt acctgatctc
      181 tgccgaggcc cgcgcctgac caacttactt cccactttac accgccatcg gtcccgtatt
      241 ttacgatcaa gacgtccgcg cccgtgacag agtctacccg gaacgtgccg acatacaaat
      301 tactgtcttc atccagtacc accggcgtct taaagaccga atcagtctgg cctgcataag
      361 cagcccagtc acggactatg gcaggcagtt gctgcgcggt actcagtaac gcacagtgca
      421 tcaggatcaa taaaacaact cctgaaaacc agattgtaaa agcagattga gcatttttca
      481 tggtatcgcg agttaaagtt tgacaagaca acaggatagt atgtgaacta aaaaatggaa
      541 agaatactat ctgagaataa acgtaaatta tattttcaaa atatgaaata ttttttccaa
      601 caaattgcat tcgtttcaaa ttcaagcaac ccaacgcacg cgtgccgaca agctagcccc
      661 gattgagcct cgtcatggga attcggtgat tcgggggatt tcgtttacgt gacgaggcga
      721 aagcgggcag gtcggtgctg cggagcgggg tgtggcgctt caaaatgttt caatttcgcg
      781 tttcgttttt ggaaaaacag gaattccgag atcatgcatt tgcatgaaaa gcgcctcgga
      841 tgtgaggaaa aacgaccaat catcggcttt cccgaaaagg gacgtttctt tttccgtgaa
      901 cggacttgag tcgctttaat ttattatctt agtttgttct caggaacgga cggtttttca
      961 cttgagacga cttcttcttt gcagcctcgg ggtgcttctt ttcctcgctg ccatcccggt
     1021 tcacgggcag ttccaacgga acggcaccgc ggtgaatact tcgtgcgatt gctaccggct
     1081 taccttgtcc gcgggcggac agaacggttc gatctggaac cagcagcgca tcgacctggg
     1141 gaattccttc gattacaact tccgcgtgaa tttcggttgc agcgacgcag gcgccgacgg
     1201 cgtggtgttc gccctgcaaa cgaacccgct cgctaccggc gtttccggcg ggggcatcgg
     1261 ctatcagacg atcagtccct cggtggcggt ggagttcgac acctatcaga acggctggga
     1321 cccgacctac gaccacatcg cgatcgtatc gaacggcgac gtggaccata ccactggcaa
     1381 caacctcgcg ggcccggtgg ccgcgctggc ctcgtcgggc aacatcgaag actgcctgtt
     1441 ccacgacgtc cgcatccgct ggaatgccgg cagcatgacg ctggaggcat acctcgacgg
     1501 cgcgttgcgg ctcagttaca ccggcaacct cgtagcgaac atcttcggcg gcaacgcgca
     1561 ggtatactgg ggcttcacct ccagcaccgg c
//
LOCUS       DAOTON010000723         1589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_61201_length_1589_cov_2.698827, whole genome shotgun sequence.
ACCESSION   DAOTON010000723 DAOTON010000000
VERSION     DAOTON010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61201_length_1589_cov_2.698827"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="P5292_12110"
     CDS             <1..851
                     /locus_tag="P5292_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39914.1"
                     /translation="ANVGLGNIIRDGTVELDSAMLRLDVINSIGADASAIIANLEATN
                     NRTGTTIPLTAPGILNTRLNINRASESGNASDPVRPTTLSLSLDRSNSNLVDLLENIP
                     DRLDYNLDLSLNPLGNVSGSNDFVYSDRLVASRLRLELPLRFAANQILLADTLDLTAD
                     ILTNLDPVGPAIVTMVAENGFPLNIELQFFLIDENGTVTDSLLQQGNLIPSAYVDGAM
                     NVVASRSTRLEIPVEAERKSRLQSARKIGIRGRFTTPAYPVLVQFYEHYRLRIKLVAE
                     GTYSIR"
     gene            929..>1589
                     /locus_tag="P5292_12115"
     CDS             929..>1589
                     /locus_tag="P5292_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39915.1"
                     /translation="MELFPEKSSVGLDAGFAFRSDCITHEFASAYFRHAFIDEQIKDR
                     VSKRLDARNAFALDFGVRTYYRHRIQSDSGRFTSWGIELEDRHWIHSDFSSDAFELYF
                     RGNRAYKGRSADLSNFRFSDLHWQAVKASVQIRLSSHSALDAGFGLVRGQEFLDIRTK
                     RGSLYTAPNGDYLDADLDLTIRQQDSSGTGFDSWNGTGAVMDLRWKKQFRDGSLFEIE
                     VS"
BASE COUNT          350 a          432 c          426 g          381 t
ORIGIN      
        1 acgctaatgt cggattaggc aatatcatac gtgacggcac cgtagaattg gacagcgcga
       61 tgctcaggct tgatgtgatc aacagcatcg gcgctgacgc gtcggcgatc atagctaacc
      121 tggaggcgac caacaatcgc accggtacca cgatcccgct aacagcgccg ggaatcctca
      181 atacccggct gaacatcaac cgtgcttcgg aaagcggcaa cgcttcggat ccggtcaggc
      241 ctaccacgct tagcttgagc ctggaccgtt cgaacagtaa cctggtggat ctgctggaga
      301 atattcccga tcgtctggat tacaatctgg acctttcact caatcccctg ggcaatgttt
      361 cgggaagcaa tgatttcgtt tacagtgacc gtctcgtggc ttcccggctt cgacttgaac
      421 taccactccg gtttgccgcc aaccagatac tgttggctga cacgctggac ttgacggcag
      481 atatcctgac taacctggac cccgttgggc ctgctatcgt gacgatggtt gccgagaacg
      541 gctttccgct caacatcgag ttgcaatttt tcctaataga cgagaacggg actgtaaccg
      601 attcgcttct gcaacaggga aacctgatcc catccgctta tgtcgatggc gcgatgaacg
      661 tggtagcctc ccgatcaacg cgcctcgaaa tcccggtcga agccgagcgg aaatcccgct
      721 tgcagtcggc caggaagatc gggatacgcg gccggttcac gacccccgct tatcctgtgc
      781 tggttcaatt ctatgaacat taccgactgc gcatcaaact agtggcagaa ggaacttatt
      841 ccatccgctg atgaccggca cgttgcatcg cctttgttta ctgttgatag tggttggatg
      901 ccgatccgcg ttcggtcagg ttgctacgat ggaacttttt ccggagaaaa gttcggtcgg
      961 actcgatgca ggattcgcgt ttcgttctga ctgcatcact catgagtttg cgtcggccta
     1021 cttccgtcat gctttcatcg acgagcagat caaggatcgt gtcagtaaaa ggttggatgc
     1081 acgcaatgcc tttgcgctgg atttcggggt aagaacctat tatcgtcaca ggatccagtc
     1141 ggattccggc cgttttacct cctggggcat tgaactggaa gatcgtcatt ggatccattc
     1201 ggatttcagc agcgacgcat ttgaattgta cttccggggc aaccgggctt acaaaggccg
     1261 atcggccgat ctctctaatt tccgattttc cgatctgcat tggcaagccg tgaaggcttc
     1321 ggtgcaaatc cgactctcca gccatagtgc gttggacgcg ggcttcggcc ttgtgcgggg
     1381 acaggaattc ttagacatcc gtacaaagcg cggaagtctg tacaccgcgc cgaatggcga
     1441 ctacctggac gccgatctgg acctaacgat acgccaacag gattcttcag gtaccggatt
     1501 cgattcctgg aacggaacgg gtgcagtcat ggatcttcgc tggaagaaac aatttcgcga
     1561 cggttccctg tttgagatcg aggtcagcg
//
LOCUS       DAOTON010000724         1588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_61274_length_1588_cov_1.979778, whole genome shotgun sequence.
ACCESSION   DAOTON010000724 DAOTON010000000
VERSION     DAOTON010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61274_length_1588_cov_1.979778"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="P5292_12120"
     CDS             <1..1283
                     /locus_tag="P5292_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39916.1"
                     /translation="TAAEYELASTTLSGITGTTNLLATFTNSNPLTVANPLKSLWLNG
                     VDIVDMLDYGYWTFTPNIATTGGTFSLSLSQRGHTNVAGRTGFTPLSRTSVGSDWQTL
                     GNSVSGSLVGGTVTTVRSGLSDFMDFGIGVAEQPKSMVFLNSSLISGTAGQVGAIYKF
                     PNVTTGIDAWVTLMDKEGGATLSDVDNFTAGNGYDIAWQPFVNHPANDTSSIGWKIQF
                     KKNGTSTDTTITNVAITGIDIDGAGSIREFVEATRPYSYSLAVVTTLTITELDTSYRA
                     TGDYPTINNIDTSHTEAMYQINYSNINTFYYRTGAISTSSSMEMRQHALMFNATLTNN
                     ASSNTSLPVSLLYFKPTAKKDRVQLDWATATEVNNDYFVIERSADGRNFEEILRKSGY
                     GNSSTTIAYTDIDYSPLSGISYYRLKQVDYDGKF"
     gene            1293..>1588
                     /locus_tag="P5292_12125"
     CDS             1293..>1588
                     /locus_tag="P5292_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39917.1"
                     /translation="MKAVRFGKTDNSRALSIESTGPNPFTSAFNIHYRSEEAGDVEIT
                     ITSATGQLVHHSTVHAELGSNDFAFTQAAELSAGLYVVTLRKGDEGVSVKLKKE"
BASE COUNT          396 a          485 c          415 g          292 t
ORIGIN      
        1 gtacggccgc cgagtacgaa ttggcatcta ccaccctgag tggcatcacc ggcactacca
       61 acctgctcgc gacctttacc aacagcaacc cgctgacggt agcgaacccg ctgaaaagcc
      121 tgtggctgaa cggcgtcgac atcgtcgaca tgctggacta cggctactgg accttcacac
      181 cgaacatcgc caccaccggg ggcaccttct cactgagcct gagtcagcgc ggacatacga
      241 atgtagcagg acgtaccggt ttcacgccac tgagcagaac cagtgtcggc agcgactggc
      301 agacgcttgg caattccgtc agcggcagtc ttgtcggcgg aacggtaacc acggtccgct
      361 ccggtctcag cgatttcatg gacttcggca tcggtgtcgc ggaacaaccc aagtcgatgg
      421 tcttcctgaa ctccagtctg atcagcggaa cggccggaca ggtaggagca atctataagt
      481 tcccgaacgt aacgaccggc atcgacgcct gggtcacgct gatggataaa gagggaggcg
      541 ccacactttc cgacgtcgac aacttcacgg ccgggaacgg ttatgatatc gcctggcagc
      601 cgttcgtcaa ccatccggcc aacgacacct cctccatcgg ctggaagatc cagttcaaga
      661 agaacggcac ctcgaccgat acgaccatta cgaatgtcgc cataacaggg atcgacatcg
      721 acggcgccgg ttcgatccgt gaattcgtgg aagcgacgcg accatacagc tattcgctcg
      781 cggtcgttac caccctgact ataacagagc tcgacaccag ttatcgcgcc accggcgact
      841 acccgacgat caacaacatc gacaccagcc atacggaagc gatgtatcag atcaattaca
      901 gtaacatcaa cacgttttac tatcgtaccg gcgccatttc caccagttca tcgatggaaa
      961 tgcggcaaca cgccctgatg ttcaatgcaa ccctgaccaa caacgcctcc tcgaatactt
     1021 ccctgccggt ttcgttgctt tacttcaagc ctaccgcgaa gaaggaccgt gtccagctag
     1081 attgggcgac ggcgaccgaa gtgaacaacg actacttcgt gatcgaacgc tcggcggacg
     1141 gacggaactt cgaagagatc ctgcgcaaga gcggctatgg caacagctcc accaccatcg
     1201 cgtacacgga catcgactac tccccgctat cgggtatcag ctactaccgc ctgaagcagg
     1261 tcgactacga cggaaagttc tagtactttc cgatgaaagc ggttcgcttc gggaagacgg
     1321 acaacagtcg tgcgctcagc atcgaaagca cggggccgaa tccgttcacc agtgcattca
     1381 acatccacta tcgatcggag gaagcgggcg atgtggaaat aacaatcacc agcgctaccg
     1441 gacaactggt gcatcactcg accgttcatg ccgagctagg cagcaacgac ttcgccttca
     1501 ctcaagccgc cgagctttca gcaggcctgt acgtcgtcac cttgcggaaa ggtgatgagg
     1561 gggtgagtgt gaagctgaag aaagagta
//
LOCUS       DAOTON010000725         1587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_61305_length_1587_cov_3.389034, whole genome shotgun sequence.
ACCESSION   DAOTON010000725 DAOTON010000000
VERSION     DAOTON010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61305_length_1587_cov_3.389034"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..>1587)
                     /locus_tag="P5292_12130"
     CDS             complement(145..>1587)
                     /locus_tag="P5292_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRS39918.1"
                     /translation="IEPHDGNDVHTTIDVNIQDVAESSLRRHLELNKADHGCAVLMEV
                     ATGEIRAIANLSRTEGGDYVEDFNYAIAEAAEPGSTFKLASLLVALEDQLVDLQDTVE
                     VGNGVYVYSRKNGTMRDAHAPRRSRMSVQEIFEHSSNVGVSRIIFSNYAKRPQEFVNR
                     LRSFGLGEKLGLQIDGEGAPFIRNANEKGWSGISLPWMSIGYECLITPLQTLTFYNAV
                     ANNGRMVKPLLVKEIRHRGQLVKSFETEVMRDSIASAEALQKAHIMLEGVVQNGTGDV
                     LKDSPYRIAGKTGTAQIAHSRFGYNKGNAQYQASFVGYFPADKPAYSCIVIVNAPSNS
                     LYYGGAVAAPIFKEIADKVYSSRLDLHNGDVPVDSNVYTPVPYVRAGAQKETRQVLAG
                     LGVSYHAASEEAAWVSATRGKDEVELQPRRVAQGAVPNVIGMGLKDALYLLESSGLLV
                     RVTGKGSVTRQSIGAGTPVRKGQQIVIELG"
BASE COUNT          339 a          477 c          419 g          352 t
ORIGIN      
        1 cggcctgcgt tccgcgcact gcaacaaaga gcgatccgtt ccgcgctttc cgcgaatcga
       61 aacagacatc cacgatctcg gtatcggtgg aaccgaagac atcgagtatg ccggctttgt
      121 agagtatgtc cttcagcggc ttcatcaccc cagttcaatg acgatttgtt gacctttccg
      181 aacaggcgtg cccgcgccaa tggattgccg tgttacactt ccttttccag tcacacgaac
      241 gagtagtccg gaagactcca gtaaatacaa cgcatcctta agacccatac cgatcacatt
      301 cggtacggca ccctgcgcca cacgacgggg ttgtaactcc acctcatcct tcccccttgt
      361 cgcgctgacc cacgccgcct cttcggacgc cgcgtggtag ctcacgccca atcccgccaa
      421 tacttgccgc gtctcctttt gtgcaccggc cctgacataa ggaacaggcg tatatacatt
      481 cgaatcaacc ggcacatcgc cattgtgcag atcaagccgg gaactgtaga ccttgtcggc
      541 gatctccttg aagatcggcg cggcgactgc gccaccatag taaagactat tagaaggggc
      601 gttgacgatc acgatgcaac tgtacgcggg cttgtctgcc gggaagtacc cgacaaaaga
      661 cgcctggtat tgggcattcc ctttgttata cccgaagcga ctgtgcgcga tctgggcggt
      721 accggttttt cccgcgatgc gatagggaga atccttcagc acgtcgcccg ttccgttctg
      781 gacaacgccc tcgagcatga tgtgcgcttt ctgaagcgcc tcggctgaag cgatcgagtc
      841 acgcatcact tccgtttcga aggatttgac caattggccg cgatggcgaa tctctttcac
      901 cagcagcggc ttgaccattc ggccattgtt agcgaccgcg ttgtagaagg ttagtgtctg
      961 caagggggtg atcaggcact cgtacccgat cgacatccag ggcaacgaaa tgccggacca
     1021 gcctttttca ttcgcgttcc ggatgaaggg agcaccttcg ccgtcgatct gcaggccgag
     1081 tttttccccc agcccgaagc ttcgaaggcg attcacaaac tcctgcggtc gcttggcata
     1141 gttggaaaag atgatccggg aaacgccgac gttggatgaa tgttcgaaga tctcctggac
     1201 actcatccgg ctgcggcgcg gcgcatgcgc atcacgcatg gtcccgttct tacgggaata
     1261 gacataaaca ccattgccga cttcgaccgt atcctggagg tcgaccaact gatcttcaag
     1321 tgccaccaac agggaagcca gtttgaaggt ggagccgggc tcggcagctt ccgcgatggc
     1381 ataattgaag tcttccacat aatcaccgcc ttccgtacgc gagaggttgg cgatcgcccg
     1441 gatctccccg gtagccacct ccatcagtac cgcacaaccg tgatcggcct tgttgagttc
     1501 caggtggcgg cgcaaggaac tctccgcaac atcctggatg ttgacatcga tcgtagtgtg
     1561 gacgtcgttt ccgtcatgcg gttcgat
//
LOCUS       DAOTON010000726         1586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_61374_length_1586_cov_2.313521, whole genome shotgun sequence.
ACCESSION   DAOTON010000726 DAOTON010000000
VERSION     DAOTON010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61374_length_1586_cov_2.313521"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="P5292_12135"
     CDS             <1..395
                     /locus_tag="P5292_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39919.1"
                     /translation="NEELPEFVTGWRGSLLGQVLVADLNQNNEPEIFIAAVDPKKLEY
                     AEFRAFERAANNYIRFDLPELTEKQNGGYRGGDLFWFDNLTICRAIRIYRTQDLDGKP
                     TGGERRIYYKVDNIPSLSFAGMQAMERQ"
     gene            complement(465..1475)
                     /locus_tag="P5292_12140"
     CDS             complement(465..1475)
                     /locus_tag="P5292_12140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39920.1"
                     /translation="MKKFYGFIALVCCALVAQAQPTLVASTSNPVVNDAFTVYSFTPT
                     ANQQGSAGANQTWNFASLSSSTTLTSTMISPTGTPYVSAHPTATIVNKTANTNYEYYK
                     TSSTIWERQGFYNLSAAYTYENAQTLVSYPLSYTGTFNDTYRGSFSIGGTIFGFRNGN
                     STVVADAWGTLTTPAASYTNTLRVKYTDSYLDSTFLSGAWQVKNFTMTTYLWFKSGIH
                     YPLMGISYVTGASTSSNAYWIRATGVGIEENLAAQLNMLVYPNPAQERSTLSLELTEA
                     SDVMVRVLDLQGREVIAIPMERYLPGKLQLPLDVANLASGNYLLQAIINDRNATIRFD
                     KQ"
BASE COUNT          375 a          398 c          463 g          350 t
ORIGIN      
        1 ccaacgaaga gctgcccgaa ttcgtgacgg gctggcgcgg cagtctgctc ggacaagtac
       61 ttgtagccga cctcaaccag aacaacgagc cggagatctt catcgccgcc gttgatccaa
      121 aaaaactgga gtatgcggag ttccgcgcgt tcgaacgcgc cgcaaacaac tatatacgct
      181 ttgatttgcc tgaactgacc gagaaacaaa acggtggtta tcgcggcggt gaccttttct
      241 ggttcgacaa cctcacgatc tgtcgcgcga tcaggatcta ccgcacccag gatcttgacg
      301 gaaagcctac cggtggcgag cgaaggatct attacaaagt ggacaacatt ccatcacttt
      361 ccttcgccgg catgcaggcg atggaacgac aataaacgta ccgccaaaaa caagaaaccc
      421 cgaaagctgt cgccttcggg gtttttcttt gtgcggggga atgatcactg tttgtcgaaa
      481 cggatcgtag cattgcgatc gttgataatg gcctgcaaca ggtagttgcc ggaagccagg
      541 ttggcgacat ccagcggaag ctgaagttta ccgggaaggt aacgttccat cggaatggcg
      601 atcacttcac gaccctggag atcgagtacg cgaaccatga cgtcggatgc ttccgtgagt
      661 tcgaggctca gcgtgctgcg ctcctgtgcc gggttggggt agaccagcat gttcagttgc
      721 gcggccaggt tttcttcgat gccgacaccg gtagcgcgga tccaataagc attcgagctg
      781 gtggatgcac cggtgacata gctgatgccc atcaacggat agtgaatgcc cgacttaaac
      841 cagaggtaag tcgtcatggt gaagttcttc acctgccagg caccgctgag gaaggtggag
      901 tcgaggtaac tgtcggtata cttcacccgc agggtgttcg tataactcgc ggccggagtg
      961 gtcagggtgc cccaggcgtc agctacgact gttgagttgc cgttacggaa accgaagata
     1021 gttcctccga tcgaaaagct accgcgataa gtatcgttga atgttcccgt atacgagagc
     1081 ggatagctca ccagcgtttg tgcgttttca taagtataag cagcggatag gttgtaaaaa
     1141 ccctgtcgtt cccagatcgt gctcgaggtc ttgtaatact cataattcgt attcgcggtc
     1201 ttgttaacga tggtggccgt cgggtgagcc gatacataag gcgtaccggt cgggctgatc
     1261 atcgtactgg tcaacgtggt gctcgagcta agcgaagcaa agttccaggt ctggttggcg
     1321 ccggcgctac cttgctggtt ggccgtagga gtgaaggaat acacggtgaa cgcgtcattg
     1381 acaaccgggt tgctggtcga tgcgaccagg gtcggttgcg cttgcgcaac cagtgcgcag
     1441 catactaagg caataaagcc gtaaaacttc ttcatggtgg gttggttgga agggccacaa
     1501 agataaccaa gcccgggtgc cctccaaacg tcctattccg cgtttgtagc ggattttcga
     1561 agaatggaag cgacaacgag ccaggc
//
LOCUS       DAOTON010000727         1585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_61433_length_1585_cov_2.413072, whole genome shotgun sequence.
ACCESSION   DAOTON010000727 DAOTON010000000
VERSION     DAOTON010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61433_length_1585_cov_2.413072"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="P5292_12145"
     CDS             <1..282
                     /locus_tag="P5292_12145"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRS39921.1"
                     /translation="MSKKAERALKRIGQLQMDKYPVCIAKTQYSFSDRPEDAKAEGGF
                     YINVDELIINSGAGFIVAVCGEIMRMPGLPENPNALGIDVKDGVIVGLV"
     gene            complement(291..>1585)
                     /locus_tag="P5292_12150"
     CDS             complement(291..>1585)
                     /locus_tag="P5292_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRS39922.1"
                     /translation="KMPVAVALGGDPAYTYCATAPLPDGVDEYMLAGFLRKKKVELVQ
                     CLTQDIQVPADADIIIEGYVDPNEDYILEGPFGDHTGYYSLPDYYPKFHVTAITHRKN
                     AVYPATIVGIPPQEDAWIGKATERIFLVPIKMTMVPEIVDMVLPVEGVFHNLVVVKIR
                     KEYPGQASKVMHSLWGAGQMMFTKMMVIVDGDVNIHDPVELARYVSANFDPLHDTYFT
                     AGPVDVLDHSCSVMAFGGKMGLDATSKSPEELSMRKTVTGTNDTTPIDLPALKIKYPE
                     IRDLNISLLEKGISLAFLSIEKNKPGHVKELTRALFALPEFRRIKCLIVLEHSVDVQD
                     IADSVWRFSNNVDPKRDCLTIESTDDGAFNHLVLDGTRKTLAHDGFQRDWPNILTSDE
                     ATIALVDQKWQEYGLGTFLPSPSNKYRKQLYAGGAVAE"
BASE COUNT          353 a          412 c          442 g          378 t
ORIGIN      
        1 atgtccaaga aggctgagcg cgccttgaag cggatcggac agcttcagat ggataagtat
       61 cccgtctgta tcgcgaagac acaatattcg ttcagtgatc gccccgaaga cgctaaagcc
      121 gagggtggat tttacatcaa cgtggacgag ttgatcatca acagcggggc cgggtttatc
      181 gtggccgttt gcggagagat catgcgcatg cccggtctgc cggagaaccc caatgcgttg
      241 ggtatcgacg tgaaagacgg tgtgatcgtc ggcctggtct gagggatagc ttattcggca
      301 acggctcctc cggcatacaa ctgcttccgg tatttgttgg aaggcgaggg caggaatgta
      361 cccagcccgt attcttgcca tttctggtca accaacgcga tcgtagcttc atcggaagtc
      421 aggatgttcg gccagtcgcg ctggaaaccg tcatgtgcaa gtgtcttgcg tgtaccgtcg
      481 agtaccaggt ggttgaacgc gccgtcgtct gttgattcga tggtcaggca atcccgtttc
      541 ggatcgacat tattggaaaa gcgccagacg ctgtcggcga tgtcctgcac atcgacggag
      601 tgttccaaaa cgatcaggca tttgatccga cgaaactccg gtaaggcgaa gagcgcacgc
      661 gtcagttcct tcacatgacc gggcttgttt ttttcgatac tcagaaacgc aagggaaatc
      721 cccttttcca acaagctgat attaagatcg cggatctccg ggtactttat cttgagggct
      781 ggaagatcaa tcggtgtggt gtcatttgtt ccggtaacgg tcttgcgcat gctaagctct
      841 tccggactct tactggtcgc gtcgagtccc attttgcctc cgaaggccat gacagaacaa
      901 ctatggtcga gcacgtcaac cggaccggca gtgaagtaag tgtcgtgcag cgggtcgaaa
      961 ttcgcggata cgtaacgggc cagctcgacc ggatcatgga tgttcacatc cccgtcgacg
     1021 atcaccatca tctttgtgaa catcatttgt cccgcgcccc atagcgaatg catcactttg
     1081 gaggcctgac ccggatactc tttccggatc ttcactacga cgagattatg aaatacaccc
     1141 tctaccggca gcaccatatc cacgatctcg gggaccatgg tcatcttgat cggaaccagg
     1201 aagatccgtt cggtcgcttt cccgatccag gcgtcttcct gcggggggat accgacaatg
     1261 gtggcgggat agacggcgtt cttgcgatgg gtaatggcgg tcacatggaa tttagggtag
     1321 taatccggca gtgaataata gccggtatgg tctccgaaag gtccttcgag aatgtagtcc
     1381 tcgttcggat ccacataacc ttcaatgatg atatcggcgt cggcgggaac ttgtatgtcc
     1441 tgggtgaggc attgtaccaa ctcaactttt ttctttcgca aaaaaccggc aagcatgtat
     1501 tcatcgaccc catccggcag cggcgcggtc gcacagtagg tataagccgg gtcgccaccc
     1561 agggctacgg ccaccggcat ctttt
//
LOCUS       DAOTON010000728         1584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_61480_length_1584_cov_2.056900, whole genome shotgun sequence.
ACCESSION   DAOTON010000728 DAOTON010000000
VERSION     DAOTON010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61480_length_1584_cov_2.056900"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1584
                     /gene="asnB"
                     /locus_tag="P5292_12155"
     CDS             <1..>1584
                     /gene="asnB"
                     /locus_tag="P5292_12155"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRS39923.1"
                     /translation="IATFDRLSLGHARLSIIDTSDAGRQPMSDPSGRYTIIFNGEFFN
                     YREHREELVRRGVSLHSNSDTEVLLHWYIPEGAACLQRVNGFFALAIYDRTERKLFVA
                     RDRMGVKPLLTYTDSDRFCFASEMKALVSMGIPMRLDRVSLFQYLQLNYVPGPESMLL
                     GVRKLEPGHYQVIRNIGENASVDEPVAYYQIPDSTFETTPGYHEAQDRLLYLLERSVE
                     RRMVADVPLGAFLSGGIDSSIIVALASGMTKKLKTFSIGFRDEPQFDETHFAELVAQR
                     YKTDHTVFKLTTDDLYSVLQPALDYIDEPFADSSALNVFLLSRETRKHVTVALSGDGA
                     DELFGGYNKHAAEWRLRNNNTATTAIRLLGPLWKQLPRSRNGKWTNRFRQLDRFSAGA
                     GMSATDRYWRWAGFEDESSVRQLFMHDSFSTDERVYQARKTRLTRFIKGGTSLNDVLR
                     NDMELVLVNDMLTKVDLMSMANSLEVRTPFLDYEVVNFASRLPGEYKIDGNGRKKVLR
                     DAFRKLLPDELYRRGKQGF"
BASE COUNT          367 a          442 c          426 g          349 t
ORIGIN      
        1 catcgcgacc tttgatcgtt tgtcgttagg gcacgcccgg ctctccatca tcgatacgag
       61 tgacgcgggc cggcaaccga tgtcggaccc ttcgggccgc tacaccatta ttttcaatgg
      121 tgaattcttc aattaccggg aacaccggga agaactggtt cgtcgtggcg tcagtttgca
      181 ttcgaatagt gataccgaag tcttattgca ttggtatatc ccggaaggtg cagcgtgtct
      241 gcaacgcgtc aacggcttct tcgcgctcgc catttacgat cgaacggaac gtaaattgtt
      301 tgtcgcacgc gaccgcatgg gtgtgaaacc cctgctgacg tataccgaca gcgatcgttt
      361 ctgcttcgcc agcgaaatga aggcattggt aagcatgggg attcccatgc ggctggatcg
      421 tgtctcactc ttccaatacc ttcagttgaa ttacgtaccc gggccggaat ccatgctgtt
      481 aggggttcga aaactggaac ccggacatta ccaagtcatc cgaaacatcg gggaaaacgc
      541 ttccgtggat gaaccggtag catactatca gattccggat tcgacctttg aaacaacgcc
      601 aggttaccac gaagcacagg accgcctgct gtaccttctg gaacgctcgg tggaacgacg
      661 catggtcgcc gacgttccgc tcggcgcctt tctcagtgga ggcatcgatt catctattat
      721 tgtcgcgctg gccagcggga tgacgaagaa gctgaaaacc ttttccatcg gcttccgcga
      781 cgaaccgcaa ttcgacgaaa cccactttgc ggagctcgtt gcccagcggt ataaaaccga
      841 tcataccgta ttcaagctca caaccgacga cctttactcc gttctgcaac cggcgcttga
      901 ttacatcgat gaacccttcg ccgactccag cgcgctcaat gtattcctgc tgagccgtga
      961 aacccgtaag cacgtaaccg tcgcgctgag cggcgacggt gccgatgaac ttttcggcgg
     1021 ctacaacaag cacgccgctg aatggcggtt gcggaacaac aacactgcta ccacggccat
     1081 ccggctcctg ggaccactgt ggaagcaact gccacgctcg cgtaacggca aatggaccaa
     1141 tcgtttccgt caactcgatc gctttagcgc cggggccggc atgtcggcca cggaccggta
     1201 ttggcgatgg gcaggcttcg aggatgagtc cagtgttcga cagttgttca tgcatgacag
     1261 tttcagcacg gatgaacggg tttatcaggc ccgcaaaaca cggttgacga ggtttatcaa
     1321 aggcggaaca tccctgaacg atgtgttgcg caacgatatg gagctggtgc tcgtcaacga
     1381 tatgcttacg aaagtcgacc tgatgtcgat ggccaattca ctcgaagtca ggaccccctt
     1441 cctggattat gaagtggtca atttcgcgag ccgcctgccg ggagaatata agatcgacgg
     1501 caacggcaga aagaaagtat tacgggatgc gtttcgcaag ctcctacccg acgagctcta
     1561 ccgacgcggc aagcagggat tcga
//
LOCUS       DAOTON010000729         1582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_61578_length_1582_cov_2.962017, whole genome shotgun sequence.
ACCESSION   DAOTON010000729 DAOTON010000000
VERSION     DAOTON010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61578_length_1582_cov_2.962017"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="P5292_12160"
     CDS             <1..452
                     /locus_tag="P5292_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRS39924.1"
                     /translation="TMKEDMLAGFKVGADDYITKPFDSEVLLYKLKAILKRSNEKGHD
                     HTQREFTVGKYQFNYDHRTIISGEESQKLSPKEADLLRMLCIYKNELLPRQKALKEIW
                     GDDNYFNARSMDVFITKLRKYLRDDPTLDIINIHGKGFRLVDNVQKP"
     gene            complement(466..>1582)
                     /locus_tag="P5292_12165"
     CDS             complement(466..>1582)
                     /locus_tag="P5292_12165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39925.1"
                     /translation="CPGSFVTPHDYIVTDPVRSGNPVIVDYELEPYTVTGGPPGFYFP
                     EYYIQSQAIYYQAPGYRNNAALLEVLRPNDAFEYNRMNPACGEQHPLVRFRNLGSDTL
                     RSLEFHYGLDGDHSTVHTWTGALPFLDTLIIELPVITQTAGSHLFSVMAVNPNGQPDE
                     FGSDDSLNCTFTTPAMYNTSFVRVLVKTDGAPNEVSTSITDPAGNVLFSRTSYPAAGS
                     LYIDTVFLSNGCYSFNVFDSFGDGLCCFGGSGYFRVLKGGTATVICNANDFGENYKKE
                     AIIDFNPGIADEPELDRVLVWPNPSTGLVHLNTSDERGLLTARVTDISGRVLSETLEL
                     PIDRHEAILDLSRLPRGLYFLELSASGWRQVVRWVRE"
BASE COUNT          426 a          412 c          403 g          341 t
ORIGIN      
        1 aaacgatgaa ggaagacatg cttgcaggat ttaaagtcgg ggctgatgac tacatcacaa
       61 agccgttcga ttccgaagtc ctgctataca aactaaaagc catcctgaaa aggagcaacg
      121 aaaaagggca tgatcacacc cagcgggaat tcaccgtggg caaataccag ttcaattacg
      181 atcatcgcac catcatctcg ggcgaagaat ctcaaaaact gtctccaaag gaggccgatt
      241 tgttgcggat gctgtgcatc tacaaaaacg agttgttacc cagacagaag gcgctcaaag
      301 agatctgggg tgatgacaac tatttcaacg cccgcagcat ggacgtgttc atcaccaaac
      361 tgcgtaagta cctgcgcgac gatcccaccc tggatatcat caacattcat ggcaagggct
      421 tccggctggt cgataatgtc cagaagccct gatcggagtt cggatttatt cccgaaccca
      481 acgcactacc tggcgccagc cgctggcgga cagttccagg aaatacaggc cgcgtggaag
      541 tcggcttagg tcaagaatag cttcgtgtct gtcaattggc aattccaatg tttcagacag
      601 cacacgaccg ctgatgtcgg taacccgggc cgttaggagt ccacgctcat ccgaggtgtt
      661 caggtgcacc agcccggtcg agggatttgg ccacaccagc accctatcca attcaggttc
      721 gtccgcaatt cccggattga aatcgatgat cgcttctttc ttgtagttct caccaaaatc
      781 gttggcattg cagataacgg ttgccgtccc ccctttcagt acccggaaat aaccgcttcc
      841 accgaagcag cacaaaccgt caccgaaaga atcgaataca ttgaaactgt aacagccatt
      901 tgacaggaac accgtatcga tatacagcga gccggcggca ggatagctcg ttcgggagaa
      961 cagcacatta ccggccggat cggtaatact cgtggatact tcattcggcg cgccatcggt
     1021 tttcaccaac accctgacaa acgaggtatt gtacatcgcc ggtgtggtga atgtgcaatt
     1081 cagcgaatca tcggacccga actcgtcggg ctggccattc ggattgacgg ccattacact
     1141 gaacagatga gaaccggcag tttgtgtgat cactggcagt tctatgatta gtgtatcaag
     1201 gaaaggcaat gcaccggtcc atgtatgaac cgtggaatgg tcaccgtcga gaccgtaatg
     1261 aaattccaga ctgcgcagcg tatcactgcc caggtttcgg aaccgtacga gcggatgctg
     1321 ttcaccgcag gcgggattca tcctgttata ttcaaaagca tcattcggac ggagaacttc
     1381 cagcagggct gcattattgc ggtaacccgg agcctggtag tagatcgcct gtgattgaat
     1441 gtagtattcc gggaaataaa atcccggagg tccgccggtt acggtataag gctccagctc
     1501 ataatcaaca atgaccggat taccggatcg cacaggatcg gtaacgatgt aatcatgcgg
     1561 ggtcacgaat gaaccggggc ac
//
LOCUS       DAOTON010000730         1580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_61690_length_1580_cov_2.967213, whole genome shotgun sequence.
ACCESSION   DAOTON010000730 DAOTON010000000
VERSION     DAOTON010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61690_length_1580_cov_2.967213"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="P5292_12170"
     CDS             complement(<1..392)
                     /locus_tag="P5292_12170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS39926.1"
                     /translation="MSNARIRFEPVTAAHWSQVEDLFGEKGACGGCWCMHWRLKASDY
                     NAGKGAGNKRRLRNLTRKFPPGILALSGKEAVGWISMGPREDFPRLSGSRILKPLDDS
                     PTWSIVCLFIRKDHRRQGLSTALINAAA"
     gene            complement(389..1240)
                     /locus_tag="P5292_12175"
     CDS             complement(389..1240)
                     /locus_tag="P5292_12175"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRS39927.1"
                     /translation="MEDRMKAQLLGVHCSIAGGLEKAFEEADALGIDAMQIFTQNQRQ
                     WVNKVLSDEAIRSFKSAWKKSGVKRIFSHCSYLLNLGSADPALFSKSVDALEGEVRRC
                     HALGLSGCVLHPGAAQGRSDEVTLARIAEGLQEVLQRTAACRTHILIENTAGQGTSMG
                     YQFRHLGTLLKTVRSNRLGVCFDTCHAFAAGYDLRDAKGFQACWKEFDQEVGMERLEA
                     IHLNDSKGGLGSRLDRHEHIGRGELGRDCFRRMLEKFPDVPKVLETDKEDDMDRKNLE
                     YLRKLIQ"
     gene            complement(1225..>1580)
                     /locus_tag="P5292_12180"
     CDS             complement(1225..>1580)
                     /locus_tag="P5292_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRS39928.1"
                     /translation="GISVLATSSNDPVQYPQDSPEEMRKHAVEMGYPFPYLFDATQEV
                     ARAFDAACTPDFFVYDTSGRLYYRGQLDDARPKSDLPVTGHDLRHALDELLRGGPAPE
                     FQRPSIGCNIKWKTA"
BASE COUNT          346 a          447 c          423 g          364 t
ORIGIN      
        1 tgtgcggcgg cattgatcag cgcagtggaa agtccctggc gacgatgatc ttttcgaatg
       61 aagaggcaga cgatgctcca ggtcggactg tcatccaaag gcttcaggat gcgggaaccg
      121 gagaggcgcg ggaagtcttc gcgcggaccc atcgaaatcc agcccacagc ttcctttccg
      181 gagagggcca gaatacccgg tggaaatttc ctcgtaaggt tccgcagccg ccttttattg
      241 cctgcccctt tgccggcatt gtagtcggag gcttttaatc gccaatgcat gcaccagcaa
      301 ccaccgcaag cacctttttc accgaagagg tcttccactt gtgaccagtg agctgctgtc
      361 acgggctcaa aacgaatcct ggcgttactc attggatcag ttttctcaaa tactccaggt
      421 ttttcctgtc catatcgtcc tccttatccg tttccagcac tttcggaacg tcgggaaatt
      481 tttccagcat gcgtcgaaaa caatcccggc caagttcacc tcgtccaatg tgttcgtgcc
      541 gatcgagtcg gctgcctaat ccgcctttcg aatcattcag gtgaatggct tccagtcgtt
      601 ccatgccgac ttcctgatcg aattccttcc aacatgcctg aaaccccttt gcatcgcgta
      661 gatcgtaacc tgcggcgaaa gcatggcagg tatcaaagca gacgccgagc cggttggagc
      721 gcacagtttt caataatgta ccgagatgac ggaattgata ccccatgctg gtgccttgtc
      781 ctgctgtgtt ctcgatcagg atatgggttc gacaagcggc ggttcgttgc agcacttcct
      841 gcaatccttc ggcgatccgg gcgagggtga cttcatcaga ccgaccttgt gcggcacccg
      901 gatgcaggac gcaccccgac aagccaaggg catgacaacg gcgtacctca ccttcgaggg
      961 catcgacaga tttcgaaaag agggcagggt ccgccgaacc caggttaagc aggtacgaac
     1021 aatgggagaa gatacgcttg actccgctct tcttccaggc cgatttgaat gatcggatgg
     1081 cttcgtccga aagcactttg ttgacccact ggcgctgatt ttgggtgaag atctgcatcg
     1141 cgtcgatgcc gagtgcatcc gcttcttcga atgccttttc caatccaccg gctatcgaac
     1201 agtgtactcc aagcaactgc gctttcatgc ggtcttccat ttgatgttgc agccgatgct
     1261 gggtcgctga aattccggtg cggggccgcc acgcagcaat tcatcgagcg catgccgaag
     1321 atcgtgcccg gtaaccggaa gatccgactt aggacgcgcg tcatccaatt gaccgcgata
     1381 gtaaaggcga cccgaggtat catacacgaa aaagtccggg gtgcaggctg catcgaacgc
     1441 gcgcgcaacc tcttgggtgg cgtcaaagag atacgggaac ggataaccca tttcaaccgc
     1501 gtgtttcctc atttcctccg ggctgtcttg aggatattgc accggatcgt tggagctggt
     1561 cgccaggacg gagataccac
//
LOCUS       DAOTON010000731         1573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_62083_length_1573_cov_3.518445, whole genome shotgun sequence.
ACCESSION   DAOTON010000731 DAOTON010000000
VERSION     DAOTON010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62083_length_1573_cov_3.518445"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1388)
                     /gene="mraY"
                     /locus_tag="P5292_12185"
     CDS             complement(129..1388)
                     /gene="mraY"
                     /locus_tag="P5292_12185"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRS39929.1"
                     /translation="MLYYLFGYLDRMFDLPGAGVFQYISFRAALAVILSLLISLVFGK
                     RIIRLLHRKQVGETVRDLGLDGQKEKQGTPTMGGLIIIAAILIPTLLFARLLNVYVIL
                     MIVTTVWLGLIGFIDDYIKVFKKDKEGLAGRFKVLGQVGLGLIVGLTLYFNPNVVVKE
                     RIREQQLTVEHKTSNVIEDIRQSEAALYAKDPVKSTKTTIPFIKNHEFDYSALLKWTG
                     PGYRNLAWLIFIPFVILIITAVSNGANITDGIDGLATGTSAIIGATLGILAYVSGNTV
                     FANYLNIMYIPYTGELVVFGAAMVGACVGFLWYNAYPAQVFMGDTGSLALGGIIATFA
                     IAIRKELLIPILCGIFLVENLSVVMQVSYFKYTKKKYGEGRRIFRMSPLHHHYQKLGY
                     HEAKIVSRFWIVGIMLAIVTMVTLKLR"
     gene            complement(1391..>1573)
                     /locus_tag="P5292_12190"
     CDS             complement(1391..>1573)
                     /locus_tag="P5292_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRS39930.1"
                     /translation="RKSLSIADRKEAIKTAVTLARSGDIILVAGKGHEKYQEIKGVRH
                     PFDDKKVIEELFKSGN"
BASE COUNT          376 a          424 c          411 g          362 t
ORIGIN      
        1 cacattgaac cccttccgga tagccagaat ggcggcaccg acaccgcttt cagcggcgcc
       61 cagcaccacc agtcgcttac gaccggatgc gttgttcgag ttggggtgtg tttccgacat
      121 gatgacggtc agcggagttt gagtgttacc atcgtgacga tcgcgagcat gatcccgacg
      181 atccagaagc gcgaaacgat ttttgcttcg tgataaccaa gtttctgata gtgatgatgc
      241 aaaggcgaca tccggaaaat ccgtcggcct tcaccatatt tcttcttcgt gtatttgaaa
      301 tagcttacct gcatgaccac cgacaggttc tcaaccagga aaatgccgca gaggatcggt
      361 atcagcagtt ctttccggat cgcaatcgcg aacgtggcga tgatcccgcc gagcgccagg
      421 cttcccgtgt cgcccatgaa gacttgtgcc ggataggcat tgtaccagag gaatcccaca
      481 caggcaccga ccatcgccgc accaaagacg accaactccc cggtgtaggg gatgtacatg
      541 atgttcaggt aattcgcgaa aacggtgtta cccgatacgt aagcaagaat gcccagagtt
      601 gcgccgatga tagccgaggt gccggtcgcc agtccgtcaa tgccgtccgt aatgttggcg
      661 ccattggaaa ctgcggtgat gatcaggatc acgaatggaa tgaagatcaa ccaggcgagg
      721 ttccggtagc ccggccccgt ccacttcagc aatgcggaat aatcgaattc gtggttcttg
      781 atgaacggaa tggtggtctt cgtcgatttg accggatcct tcgcatacag tgccgcttcg
      841 ctttggcgga tatcttcaat gacattcgaa gtcttgtgtt ccacggtcag ttgttgctcg
      901 cggatgcgct ccttcacgac gacattcgga ttgaagtaca aggtcaaacc cacgatgagc
      961 ccgagtccga cctgaccgag taccttgaac cgaccggcaa ggccttcctt gtccttcttg
     1021 aaaaccttga tgtaatcatc tatgaatccg atgagcccta gccaaacggt ggtcacgatc
     1081 atcagaatga catagacatt caacaggcga gcgaagagga gggtcgggat caggatagcg
     1141 gcgataatga tcaaaccacc catcgtcggt gtaccctgct tttccttttg accgtccagg
     1201 ccaaggtcgc gtacggtttc ccctacttgc ttgcgatgca acaaccggat gatgcgcttg
     1261 ccgaatacca gcgaaatcaa taacgaaagg atcactgcga gagcagcgcg gaaggagatg
     1321 tattgaaata cccccgcccc cgggagatcg aacatgcgat cgagatagcc aaacaggtag
     1381 tagagcatgt tcagtttccg gatttaaaaa gctcttcgat tactttcttg tcgtcgaatg
     1441 gatgacgcac tcctttgatc tcctggtact tctcatgacc cttgcccgcg accaggatga
     1501 tatctccgga ccgcgccagt gtgaccgccg ttttaatcgc ttctttgcga tcggcaatgc
     1561 tgagcgattt tcg
//
LOCUS       DAOTON010000732         1573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_62089_length_1573_cov_3.203557, whole genome shotgun sequence.
ACCESSION   DAOTON010000732 DAOTON010000000
VERSION     DAOTON010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62089_length_1573_cov_3.203557"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="P5292_12195"
     CDS             <1..595
                     /locus_tag="P5292_12195"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39931.1"
                     /translation="KPVVITSVRKINQPNTHPDVTDERIPYWAELWPAAIGLAEYLSL
                     HPSLVMGKKVVEIGCGLGLPGIVAGRLGAEVLLTDYLSEPLELARYNWERNIQRPASV
                     RQFDWRQGEDLPEADLILASDIAYESRAFPFIRTLIGHYLDKGSVILISEPNRTFAAE
                     FFKSLSLLPGYSHHTIRVVRNQLTIQVNVHRIVPERA"
     gene            776..>1573
                     /locus_tag="P5292_12200"
     CDS             776..>1573
                     /locus_tag="P5292_12200"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRS39932.1"
                     /translation="MKKLLLLLLGFVGLQLQLSAQSLTAITPNSGTVGQNQITTTITG
                     SNIFFTSISQWGNLENVELRNGSGSISVFNYMGGNWNAFVNTQDVADVTWDIPTNAVA
                     GVYDVCVTTLDPGMPWVYYYDTLYSAFTINPPDGYVSGSLYYDANANGIRDGGETGLS
                     NMVVQLTGPSNYTVYTNTNGDFSFGVANGSYTLTAGSPGRQYHFTSPSTIPVTVSNNN
                     TSGLDIGAIDPLYAMSPRVGFRGQANINATVNAYDNIFTTSGNPWGNI"
BASE COUNT          373 a          405 c          401 g          394 t
ORIGIN      
        1 gaagccagtc gtgataacca gtgtaaggaa gatcaatcag ccgaacacgc acccggatgt
       61 aacggatgaa cggataccct attgggcgga gctctggccc gccgcgatcg gattagcgga
      121 gtacctttcc ctgcacccgt ccctggtgat gggaaagaaa gtcgttgaaa tcggctgtgg
      181 gcttggactg cccggcatag tggcgggaag gctgggtgcg gaagttttgt tgacagatta
      241 tctttccgaa cctttggagt tggctcgcta caattgggaa cgaaacatcc aacgaccggc
      301 cagtgtacgc cagttcgatt ggaggcaagg cgaagatctt ccggaggccg atcttatcct
      361 ggcttccgat attgcttatg agtcccgagc ttttccgttt atccgaacct tgatcggaca
      421 ttacctcgac aaaggatccg taatactgat cagcgaaccc aaccgtacat tcgcagcgga
      481 gttcttcaaa tcgttgtcgt tgcttccggg ttattcccat cacacgatcc gggtagtgcg
      541 gaaccagctt acgatccagg tcaatgttca ccggatcgtt ccggagagag cctgaggcag
      601 gttacttcca tttcttttat cctgggcaat cgattgatcg gtttcggtca cccacgggaa
      661 agatgcattc acctatgaaa actgtttcat catcaaaata cttggatttt gattcgtttt
      721 tctttgtgtg tatcaaccac ttgcctactt ttcgccctgt ctaaccacac tgaccatgaa
      781 aaaactttta ctgctcctgc tgggcttcgt cggcctgcag cttcaactct ccgcccaatc
      841 gttaacggcg atcacgccga actccggaac agtcggacag aaccagatca cgaccacgat
      901 caccggtagc aacattttct tcacaagtat cagtcagtgg ggaaacctgg aaaatgtcga
      961 gttgcgcaac ggatccggta gcattagtgt gttcaattat atgggtggca actggaatgc
     1021 ctttgtgaac acccaggatg tggcggatgt tacatgggat attcctacca atgcagtggc
     1081 cggggtttac gacgtttgtg tgacaaccct tgatccgggt atgccctggg tctactatta
     1141 tgatacgctg tacagcgctt tcacgatcaa tccgccggat gggtatgttt ccggaagctt
     1201 gtactatgat gcaaacgcca atgggatacg agatggtggt gaaaccggct tgtcaaacat
     1261 ggttgtccaa ttgaccggac cgtccaacta caccgtctat accaatacca atggagattt
     1321 cagtttcggt gtcgcgaatg gatcctacac cttgactgca ggttcgcccg gacggcagta
     1381 tcattttacc agcccgtcca caataccggt cacggtgagc aataacaata cgagcggcct
     1441 ggatatcggc gcgatcgatc ccttgtacgc gatgtcgcct cgcgtgggtt ttcgggggca
     1501 agccaatatc aacgcaacgg ttaacgcgta cgacaacatc ttcaccacca gtggtaatcc
     1561 ctggggaaat atc
//
LOCUS       DAOTON010000733         1570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_62290_length_1570_cov_2.733993, whole genome shotgun sequence.
ACCESSION   DAOTON010000733 DAOTON010000000
VERSION     DAOTON010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62290_length_1570_cov_2.733993"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1570
                     /locus_tag="P5292_12205"
     CDS             <1..>1570
                     /locus_tag="P5292_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39933.1"
                     /translation="NNAWVPLANAPGTWSFSSVNVVGNYFFPNQLGSNPVVFTYTDSL
                     GCSASITKYINVIACCLDTCDANAGPDQVICAGQPLTLQTAGCDSTTWFEIFEGELQP
                     VGQGPIIDLFPMQSTCYVLVCSNGCCSDTDTVCVTVNPVPILQWPYFLSSICVDAGPQ
                     ALSDSSILVWQGNAWIPANLAAGTGYFTGPGIFANVFTPPGIGSFLVSYTFVDTNGCS
                     TTVTNAITVIQCGGCDTAACNCNNVPPPPPPTITIVSVDPDNCFNDGCIHATFTGCCL
                     LFSYSYFDPCNPLLSYSIAQTPDSNIFCNLRAGNYIIYVQDACGNFTQQSVTVPLASG
                     PLTAILNFSQCGDSVCVAVEGGCAPYTFDWGNGPTTDQCIALEERCVMRTVTITDARG
                     CSVTKTITVPRFDFVAVQPHCCLNNGSICIEGCFGPQPYFYSWSNGATTSCIAGLGAG
                     TYCVTVTNRDGEQFTCCYTLSDAGFVPPSVSFNFSNCGSMVTAVINGNGCGNYSYHWQ
                     NNSTEPFFEGANACD"
BASE COUNT          312 a          434 c          422 g          402 t
ORIGIN      
        1 aacaatgcct gggtgccgtt ggcgaacgcg ccgggaacct ggagtttctc gagtgtgaat
       61 gtggtcggga actatttctt cccgaaccaa ttgggctcca acccggttgt tttcacctat
      121 accgacagcc tcggttgcag tgcgtcgatt acgaagtaca tcaatgtaat cgcctgttgt
      181 ctggatacct gtgacgccaa tgcgggacct gaccaggtga tctgcgccgg tcaaccactc
      241 accttgcaaa cggcgggctg tgattcgacg acctggttcg agatcttcga aggtgaactg
      301 caaccagtag ggcaggggcc aatcatcgac ctcttcccga tgcagagtac ctgttatgtc
      361 ctggtgtgct cgaacggttg ttgctcggat acggataccg tctgtgtaac cgtgaacccc
      421 gtgccgatcc tgcaatggcc gtatttcctt tcttcgatct gcgttgatgc gggcccgcag
      481 gcattgagtg attcttcgat cctggtttgg cagggaaatg cctggattcc ggcgaacctc
      541 gctgccggaa ccgggtactt caccggtccg ggcattttcg cgaatgtctt caccccaccg
      601 ggtatcggtt cattcctcgt cagctacact tttgtcgata ccaacggttg ctcgactacg
      661 gtgacgaacg cgatcacggt catccagtgt ggcggttgtg atacggcagc atgcaactgt
      721 aacaatgttc cgccgcctcc gccaccgacc attaccatcg tcagcgtcga tcccgacaat
      781 tgcttcaacg acggttgcat tcatgccacg ttcacgggtt gttgtctcct gttcagctat
      841 tcctatttcg atccgtgcaa cccgctctta tcgtacagca tcgcgcagac gccggattct
      901 aatatcttct gtaacctgcg ggccggtaac tatatcatct atgttcagga tgcctgtggc
      961 aatttcacgc agcagagtgt gaccgtcccc ttagccagcg gtccgttgac ggccatcctg
     1021 aatttttcgc agtgcggaga ttcggtttgt gtggcagtgg agggcggatg tgctccttac
     1081 accttcgatt ggggcaatgg ccctactacg gatcaatgca ttgccctgga ggagcgttgt
     1141 gtcatgcgta cagtgaccat caccgatgcc cgtggttgca gtgtaacgaa aacgatcacg
     1201 gttccgcgct ttgatttcgt agcggtccag ccgcattgct gcctgaacaa cggcagtatc
     1261 tgcatcgagg gttgcttcgg accgcaaccg tatttctact cctggagcaa cggagccacg
     1321 acttcctgca tagcaggact gggtgcggga acgtattgtg taaccgttac caatcgtgac
     1381 ggagagcaat tcacctgttg ctacaccttg tcggatgccg gatttgttcc gccttccgtc
     1441 agtttcaatt tctccaactg cggcagcatg gtaacagcgg tgatcaacgg aaacggatgc
     1501 ggcaattatt cttatcactg gcagaataac agcactgaac cgttcttcga gggcgcgaat
     1561 gcctgtgatt
//
LOCUS       DAOTON010000734         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_62735_length_1563_cov_2.065650, whole genome shotgun sequence.
ACCESSION   DAOTON010000734 DAOTON010000000
VERSION     DAOTON010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62735_length_1563_cov_2.065650"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="P5292_12210"
     CDS             <1..314
                     /locus_tag="P5292_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39934.1"
                     /translation="SLDKVSVNRKMNGWVEIVHKKSGDQLNIYLEPENGAWFFFSYSR
                     GLLQTIASYSAFNDEINKLKPEKRVSKVKDKPDFEYMLSTDRAVRNFLKRMNPPPPEG
                     E"
     gene            411..1049
                     /gene="plsY"
                     /locus_tag="P5292_12215"
     CDS             411..1049
                     /gene="plsY"
                     /locus_tag="P5292_12215"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRS39935.1"
                     /translation="MLTPVNLFGLLLAYLVGSIPSAVWVGRTFYGIDVREYGSGNAGA
                     TNTFRVLGKKPGIVVLIMDVLKGFAGVKLAYLVGDYDPASPEFIDFELALSVCALLGH
                     IFPVYVGFRGGKGVATMLGILVAVHTPAALICAGVFLLSLLITGYVSLSSMAAGLAFP
                     VVIMVFYSTNSSINIFSLAVAIMILVTHQRNIERLIAGEESRVKWHPRNKIA"
     gene            complement(1011..>1563)
                     /locus_tag="P5292_12220"
     CDS             complement(1011..>1563)
                     /locus_tag="P5292_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39936.1"
                     /translation="SALGDWKFFHYFRGKNMEDLLLARLSDDRLSDADNKIERLFPRS
                     EADDVSSKEALDRAHTFEERRELGNALQAYDLAIYYNPYNALAYLARGSFKLNRLDLD
                     FATAESAIRDFSRSIRLNSTISQSWYLRANALAYLNRRERVCGDALRAFQLDTGLRDG
                     CLNLTLRICPDSSYLITGMPLHP"
BASE COUNT          354 a          410 c          427 g          372 t
ORIGIN      
        1 gtagtctcga taaggtatcc gtcaaccgaa agatgaatgg atgggtcgag atcgtgcata
       61 agaagagtgg agatcaattg aatatctacc tcgaacctga aaacggcgcg tggttctttt
      121 tcagttactc gcgcggatta ctgcagacca tcgcgtccta cagtgctttc aacgacgaga
      181 tcaacaagct gaagccggag aagcgcgtaa gcaaggtgaa agacaagcct gattttgaat
      241 acatgctctc caccgatcgg gcggtccgga acttcctgaa gcggatgaat ccaccgccac
      301 cggaaggtga ataatcgaca tcaacccgct taaaaccccg aattccgcga ttgacgcgaa
      361 ttcggggcct tttttaactt gcggggcttt agcgccctga acaacatcct gtgctgacac
      421 ccgtcaacct gttcggtcta ttactggctt acctcgtcgg gtccattccg tccgcggttt
      481 gggtcggaag gaccttttat ggcatcgatg tacgggaata tggcagcggc aacgcgggtg
      541 ccacgaatac gttcagggtc ctcggtaaga agccgggcat cgtagtactg atcatggatg
      601 tgctgaaggg attcgcaggg gtgaagctgg cttacctggt cggcgattat gatccggcat
      661 ctccggaatt catcgatttc gaattggccc tgtccgtatg cgccttgtta gggcacatct
      721 ttccggttta tgtcggcttc aggggtggaa aaggggttgc caccatgctg ggcatactgg
      781 tcgcggtaca cacgccggca gcgttgatct gcgccggtgt atttttactc tcgctgttga
      841 tcaccggcta cgtttccctg agcagtatgg cggccggctt ggcttttccg gtggtcatca
      901 tggtttttta ttccaccaat tccagtatca acatcttttc actggcagtg gcgatcatga
      961 tcctggtgac gcaccagcgc aatatcgagc gattgatcgc cggcgaggag tcacgggtga
     1021 agtggcatcc ccgtaataag atagcttgag tccggacaga tccttagcgt caggttgagg
     1081 cacccatcgc gcagaccggt gtcgagctga aatgcccgca gtgcatcacc gcacacccgt
     1141 tcccgacgat tcagataggc gagcgcattt gccctgagat accagctttg ggaaatcgtt
     1201 gaattcagcc ggatcgaccg gctgaagtcc cgtatcgctg actcagcagt tgcgaaatcc
     1261 agatccagtc ggttaagctt aaatgatccc ctggccagat aggcaagggc attataggga
     1321 ttataataaa tggcgaggtc gtacgcctga agcgcatttc ccagttcacg acgttcctca
     1381 aaagtatgtg cacggtccaa tgcttccttc gacgatacgt cgtccgcctc cgagcgggga
     1441 aacaaccgct cgatcttatt atccgcatcg ctcagccgat catcggacag cctggccagg
     1501 aggagatcct ccatgttctt gcctctgaag taatgaaaga acttccagtc acccagtgcg
     1561 gag
//
LOCUS       DAOTON010000735         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_63074_length_1557_cov_3.005326, whole genome shotgun sequence.
ACCESSION   DAOTON010000735 DAOTON010000000
VERSION     DAOTON010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63074_length_1557_cov_3.005326"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="P5292_12225"
     CDS             <1..792
                     /locus_tag="P5292_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRS39937.1"
                     /translation="IVIELGSHTDSRPIPMTNDTLSQRRAESVVKYLIKSGIEAERLV
                     ARGYGASQPRSLDRDMGSFKKGDVLNDPFISGLKSVKLKEEAHQLNRRTEFKVLRTNF
                     VKGQTGSENINLNEPASGLIIENNDLKAKEDASAPTKVEVKEVQKTEAPKVEEKKGPG
                     EIYTCKKGDTYTAVAKQYSMTVKDLKALNGLKSEVIYEGMELKVDPAGDYSEYDKKFT
                     TLEKGDDSWKALAKRLSLKDSDLKKLNKGIDDDSFRPGKRIRIAK"
     gene            907..>1557
                     /locus_tag="P5292_12230"
     CDS             907..>1557
                     /locus_tag="P5292_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HRS39938.1"
                     /translation="MTGGDLYLSRGASASKEDVHNAIRKLDKGLFPKAFCKIVPDTLS
                     GSEDHCLIMHADGAGTKSSLAYAYWKATGDLSVWKGIAQDAIVMNTDDLLCVGATDNI
                     LLSSTIGRNKRLIPGEVIQALIEGTEAICEQLRSFGIDIRLTGGETADVGDLVRTVIV
                     DSTVVSRMRRDQVISNDRIRAGDVIIGLASYGQTSYETEYNGGMGSNGLTSARHDVF"
BASE COUNT          409 a          444 c          416 g          288 t
ORIGIN      
        1 attgtgatcg aactcggatc acacaccgac tcccgcccga tcccgatgac caacgacacg
       61 ctctcccagc gtcgcgcaga gtcggttgtg aaatacctga tcaaatccgg catcgaagcc
      121 gaacgcctgg tcgcccgtgg ttacggcgct tcccagccgc gttcactcga tcgtgacatg
      181 ggcagtttca agaagggcga tgtgctgaac gacccgttca tcagcggctt gaaatccgtc
      241 aaactgaaag aagaggcgca tcagctgaac cgtcgtaccg aattcaaggt actgcgcacc
      301 aacttcgtca aaggacaaac cggctccgag aacatcaacc tgaacgaacc ggccagtggt
      361 ctgatcatcg agaacaacga cctgaaggcg aaagaagacg ccagcgcacc caccaaggtc
      421 gaagtgaagg aggtccagaa aaccgaagcg cctaaagtgg aggagaaaaa aggacccggc
      481 gagatctaca cctgcaagaa aggcgacacg tacaccgccg tagccaagca gtacagcatg
      541 accgtgaaag acctcaaagc cctgaacggc ctgaagtcgg aagtgatcta tgaaggcatg
      601 gaactcaaag tggatccggc cggcgattat tccgaatacg ataagaagtt caccacgctt
      661 gagaaaggcg acgacagttg gaaagcgctt gcaaaacgac tgagtctgaa agattccgat
      721 ctcaagaagc tgaacaaggg catcgacgac gacagcttcc gaccagggaa gcggattcgg
      781 attgcgaaat aattgaaaga gaaaggccac ccgaacggtg gcctttctct tttcggcaca
      841 tggacagcat gggagccata aacccatcca ccttcttcca tcgcctttcc gtatctttgc
      901 atactcatga caggcggcga tttatacctt tcccgtggcg ccagcgcttc caaggaagat
      961 gtgcataacg ccatccgcaa gctcgacaag ggcctgttcc cgaaagcgtt ttgtaagatc
     1021 gttcccgaca ccctttcggg aagcgaggac cactgcctga tcatgcatgc cgatggcgcc
     1081 ggcaccaaat cctcccttgc ctatgcctat tggaaggcca ccggcgacct gagtgtatgg
     1141 aaaggtatcg cccaggatgc gatcgtcatg aataccgatg atctcctttg cgtaggcgcg
     1201 acggacaata tcctgctctc ctctaccatc ggacgcaaca agcgactgat tcccggggaa
     1261 gtcattcaag ccttgattga aggtaccgaa gccatctgcg aacaactgcg ttcgtttggc
     1321 atcgatatcc ggctcaccgg aggcgagacc gccgacgtgg gcgacctggt ccgtacggtg
     1381 atcgttgatt caacggttgt aagtcggatg cgccgcgacc aggtgatctc caacgaccgg
     1441 atccgtgcgg gtgatgtgat catcgggctg gcttcctatg gacaaacatc ctacgagacc
     1501 gaatacaatg gcggtatggg cagcaacggc cttaccagcg cccgacacga cgtcttc
//
LOCUS       DAOTON010000736         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_63087_length_1557_cov_2.572570, whole genome shotgun sequence.
ACCESSION   DAOTON010000736 DAOTON010000000
VERSION     DAOTON010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63087_length_1557_cov_2.572570"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="P5292_12235"
     CDS             complement(<1..521)
                     /locus_tag="P5292_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39939.1"
                     /translation="MKSRLILIGIILVLATPAWMRIAWEFSPKRVLNVVILDKTVLNS
                     NSYKHRSINWILDYEKYARHDSSFYDINQDYYGFFPGENEKYAIRDFDTMNDAVLDSV
                     AERSDLLYCADTYGVLGNEWYRHRDRNEVSESIYGGMSEKDMQFMRKMKERKKPIVVE
                     FNSIGFPTPRDVRR"
     gene            627..>1557
                     /locus_tag="P5292_12240"
     CDS             627..>1557
                     /locus_tag="P5292_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRS39940.1"
                     /translation="MAITRIFVVEDDQFIGNLIKKALEKMDNVDVTHFLTPEECLQQL
                     HLNPDIVTIDYLLPGMNGLELMAKIKNYNADIHCIMVSGQDKLDVVIETYRQGAVDYI
                     IKNDNAIVNLENSVRNLVKTVGLRQENESLKDLVIDRNKYNTILGNSGSVMRVLRLIQ
                     KVEKSNIMVLVTGQSGTGKELVAKSIHYNSPRARKPFVTVNMGAIPADLIESELFGHE
                     KGAFTDARERRIGKFEEANEGTIFLDEIGEMDLLMQTKLLRVLQEKEVTRIGSNKSIK
                     LDVRVVAATNRNLAHEVKEGKFREDLYYRLQGFL"
BASE COUNT          406 a          423 c          400 g          328 t
ORIGIN      
        1 cgacgaacgt ccctgggtgt ggggaacccg atggaattga actccaccac gatcggcttc
       61 ttccgttcct tcatcttgcg catgaactgc atgtccttct cggacatacc accgtagatg
      121 ctttccgaca cctcgttccg atcccggtgc ctgtaccatt cattgccgag tacaccgtag
      181 gtatccgcac agtacagcag atcggatcgc tccgccacgc tgtcgagcac ggcgtcgttc
      241 atggtatcga agtcacggat ggcgtacttc tcgttctcgc cggggaagaa accgtaataa
      301 tcctggttga tatcgtaaaa cgagctgtcg tgccgggcgt atttttcgta atccaggatc
      361 cagttgatcg aacgatgctt gtagctgttg gagttcagga cggtcttatc cagaatgacc
      421 acattcaata cccgtttggg gctgaattcc catgcaatcc gcatccaggc cggggtggcg
      481 agcacgagga tgataccgat caggatcaga cgacttttca cgtaaactgt aagaaacgac
      541 gcgtaaaagt attggaattc agctccccgg gcgcaacatc cggaggcgtt tttccctaaa
      601 aagattcaca aaacactgtt aacaccatgg cgatcacccg catattcgta gttgaagacg
      661 atcagttcat cgggaacctg atcaaaaagg cgctggagaa gatggacaat gtcgatgtca
      721 cgcatttcct gaccccggaa gagtgcctgc agcaactcca cctcaacccg gatatcgtca
      781 ccatcgacta cctgcttccc ggcatgaacg gattggagtt gatggcgaag attaaaaatt
      841 acaatgctga catccattgc atcatggttt ccggccagga caaactggat gtggtcattg
      901 agacctaccg tcagggcgcg gtcgattaca tcatcaagaa cgacaatgcg atcgtcaacc
      961 tggagaattc cgtccgcaac ctcgtgaaga ccgtaggcct tcgtcaggag aatgaaagcc
     1021 tgaaagacct ggtcatcgac cggaataagt ataacaccat actcggcaac agcggttccg
     1081 tgatgcgcgt ccttcgcctc atccagaagg tcgagaagag caacatcatg gtactggtaa
     1141 ccggacagag tggaaccgga aaggaactcg tcgcgaagtc gattcactac aattccccac
     1201 gggcccggaa gccattcgtc acggtcaaca tgggagccat cccggccgac ctgatcgaaa
     1261 gcgaactctt tggtcacgag aaaggcgcgt tcacggatgc ccgtgagcga cgcatcggaa
     1321 aattcgaaga agccaacgaa ggcacgatct tcctggacga gatcggcgaa atggatctgc
     1381 tcatgcagac caagctgctg cgtgtattgc aggagaaaga agtcacccgc atcggaagca
     1441 acaaatccat caagctggat gtccgcgtcg tagcagccac caaccggaac cttgcgcacg
     1501 aagtgaagga aggcaagttc cgcgaagacc tttactaccg tcttcagggt tttctca
//
LOCUS       DAOTON010000737         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_63142_length_1556_cov_2.931379, whole genome shotgun sequence.
ACCESSION   DAOTON010000737 DAOTON010000000
VERSION     DAOTON010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63142_length_1556_cov_2.931379"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="P5292_12245"
     CDS             complement(<1..392)
                     /locus_tag="P5292_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39941.1"
                     /translation="MMTTARAQQHVHSQEPADTTTHYSAEKHLSNIRQLTFGGDNAEA
                     YFSFDSKSIVFQATNPAWNASCDQIFSSAIEPFKPALVSTGLGRTTCSYFLPGDSLFL
                     YASTHGAGKECPPKPAPRADHKYVWALYP"
     gene            585..>1556
                     /locus_tag="P5292_12250"
     CDS             585..>1556
                     /locus_tag="P5292_12250"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HRS39942.1"
                     /translation="MKLYTKLLSGLAGILLLSGNYACKQAGSTETANIREDDFLVQWL
                     DSSVQPGDDFFKFATGNWLKNNPIPASERRWGIANLVRDDNFEKLRGLCEQAANDQAA
                     QPGSNTQKIGDFWATGMDSVKIDQLGLKPLQEELGLIDQVRTKDDVLRLVARHQQFAG
                     SPMFALAIYQDEMNSSRYSLHLYQGGIGLPDRDYYFNSDSRTKEIRAAYLDHLGKMFR
                     LMGEEPALADRHAKTIMKLETDLARASRKLEDLRDPYANYNKMSLAEANAMSPGIDWK
                     SMLDSLNIQGIDTVIVGQPEFFKALANNLKTASLEDWKTYLRWNLINS"
BASE COUNT          377 a          389 c          425 g          365 t
ORIGIN      
        1 ggataaagtg cccatacata cttgtggtct gcacggggtg ccggctttgg cggacactcc
       61 ttcccagcac catgcgtgct ggcatagaga aaaagactgt cgcccggcag gaagtaggag
      121 caggtggttc tccccaaacc agtactcacc agggctggtt tgaacggttc gatcgccgag
      181 gaaaagatct ggtcacagct tgcattccag gccgggttgg ttgcctggaa gacaatgctt
      241 ttgctgtcaa aactgaaata ggcttccgca ttgtcgccgc caaaggttag ctgacggatg
      301 ttcgacaggt gcttttcagc ggaatagtgg gtagtggtgt cggccggttc ctggctatga
      361 acatgctgtt gtgcacgagc ggtagtcatc atgcctgaaa ttatccctgc cgctatggct
      421 attttctgaa ggttcatcgt gctgacgttg tgtggaactg cgaacatagt acaagggaaa
      481 agtagaatcg tcagtttatc atcattttgt aaaacacgtt aatgtgcgtt aaatccggcc
      541 tccggtaggt aaagtcccta gttttgcagg gtatccacaa acccatgaag ctctacacca
      601 aactcttatc cggccttgca gggattctcc tgctgtccgg caactatgcc tgcaaacagg
      661 ctggatcgac tgaaactgcg aatatccgcg aggatgattt cctggtgcaa tggttggatt
      721 ccagcgtaca gcctggcgac gactttttca agtttgctac cggcaactgg ctaaagaaca
      781 atccgattcc tgccagcgag cgtcgttggg gtattgccaa cctggtgcgc gacgacaact
      841 tcgaaaaact tcgtggcctt tgtgagcagg cagcgaacga tcaggctgca caacccggat
      901 cgaatacaca gaagatcggt gacttctggg cgaccggaat ggattccgtc aaaatcgatc
      961 aactcggact aaagccgctc caggaggaat tggggttgat tgatcaggtc cgaacaaagg
     1021 atgatgtgct tcggttggta gcccggcacc aacaattcgc ggggtcgccc atgttcgcgt
     1081 tggcgattta ccaggatgag atgaattcgt cccggtattc cctgcacctg tatcagggtg
     1141 gaatcggttt acccgaccgc gactactatt tcaattcaga ctcacggacc aaagaaatac
     1201 gtgcggcgta ccttgatcac ttaggcaaga tgtttcgcct gatgggagag gagccggcat
     1261 tggctgaccg tcatgcgaaa acaatcatga agcttgaaac cgatctggcc cgggcatccc
     1321 gtaagttgga agaccttcgt gatccctatg cgaactacaa caagatgtcg ctggctgaag
     1381 ccaatgcgat gtcgccgggt atcgactgga aatccatgct cgacagcctg aacattcagg
     1441 gtatcgatac ggtcatcgtc gggcaaccgg agtttttcaa ggcgctggcc aataacctga
     1501 agaccgcttc cctggaagac tggaaaacct atctgcgctg gaacctgatc aactcc
//
LOCUS       DAOTON010000738         1554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_63236_length_1554_cov_3.741828, whole genome shotgun sequence.
ACCESSION   DAOTON010000738 DAOTON010000000
VERSION     DAOTON010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63236_length_1554_cov_3.741828"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="P5292_12255"
     CDS             complement(<1..446)
                     /locus_tag="P5292_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS39943.1"
                     /translation="MYLQRLSLINFRNYEQADLVLDPSFNAFVGDNGSGKTNLLDAIH
                     YLSLCKSYFNSIDSQQIRHGEDYFMVQGQFDLDGREDVIACTVRKNQKKIFRRNQEDY
                     ERLSDHIGLLPLVMIAPTDTQLITEGSEERRRFMDAIISQYDRLYL"
     gene            600..>1554
                     /locus_tag="P5292_12260"
     CDS             600..>1554
                     /locus_tag="P5292_12260"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS39944.1"
                     /translation="MEEEEFPNPNEESSRIRHSIERYEEMVRNKEAYFFDVDAFLNII
                     DYYIERNDPVKALQVVDFAQSQHPTSIDFLLRQAQLLAMVDRTEEALAILDKAEQLTP
                     ADPDLHMIRGSIYSSLQQFEKAVACFNQAIPLADELDILYLNLAYVYESWGDYNKAIE
                     YLQLSLEDNPENDVALYEIAFCYEFTDRLEDSISFYERFIDKQPYSYAAWYNLGNVLN
                     RMDRFEEALDAYDYCLAIKDDFSSAYFNKANSLANLNRFQEAIECYKKTFEFEAPDAL
                     TYYNIGECYEQLEDMQRSREYYRKATKLDPNLSQAWLGIGVT"
BASE COUNT          390 a          427 c          384 g          353 t
ORIGIN      
        1 tccagataca gacgatcata ctgggatatg atcgcatcca tgaacctacg tcgctcttca
       61 ctgccttccg taatgagttg ggtatcggtg ggcgcgatca tcaccagggg cagtaagccg
      121 atatgatcgg acaagcgctc ataatcttcc tggttcctgc gaaatatctt cttctggttc
      181 ttgcggaccg tacaggcgat cacgtcttcc ctgccgtcaa gatcgaactg gccctgcacc
      241 atgaagtagt cttcaccgtg acggatctgc tggctgtcga tgctgttgaa gtagctcttg
      301 cataacgaca ggtaatggat cgcgtcgagc aggttggttt tgccggatcc attgtcccca
      361 acgaacgcgt tgaaggaagg gtccagaacc agatccgcct gctcatagtt acggaaattg
      421 atgagcgaaa gtcgctgcag gtacattggt gtaaagatac gcagaagtgg ccgccggagc
      481 ttcgccaacc ccgaaatcag gccaattcac ccattcgtgt ggataactcc ccctttcctt
      541 gggcgcttgc agacccaatt tcttatcttt gaagagagaa acggactttt ccagaataca
      601 tggaagaaga ggaattcccc aacccgaacg aggaaagcag ccgcatccgt cattcgatcg
      661 agcgatatga ggagatggtg cggaataaag aagcgtactt cttcgacgtc gacgctttcc
      721 tgaacatcat cgactattac atcgaacgga acgatcccgt aaaggcgctc caggtggtgg
      781 atttcgcgca gtcgcaacac cccaccagca tcgacttcct gctccgtcag gcacagttgc
      841 ttgccatggt ggaccgaacc gaagaagccc tggcgatttt ggataaagcg gaacaactca
      901 caccggcgga tcccgacctt cacatgatcc gggggagcat ctactcttcc ctgcaacagt
      961 ttgaaaaggc agttgcctgc ttcaaccagg ccattccgct cgccgatgaa ctggatattc
     1021 tttacctcaa ccttgcctac gtttacgaga gctggggcga ttacaacaag gccatcgaat
     1081 acctgcagtt gagcctggaa gacaatccgg aaaacgatgt cgccttgtac gagatcgcgt
     1141 tctgttacga gttcacggac cgtctcgaag acagcatttc cttttacgag cgcttcatcg
     1201 acaagcaacc ttacagttac gctgcctggt ataatctcgg caacgtactg aatcgcatgg
     1261 accgtttcga agaagccctg gatgcctacg actactgcct ggccatcaag gacgacttct
     1321 cgtcggccta tttcaataaa gcaaactccc tggccaacct gaaccgtttt caggaagcca
     1381 ttgaatgtta caagaagact ttcgagttcg aggcccccga cgcgcttacc tattacaaca
     1441 tcggtgaatg ctacgagcag ttggaagaca tgcagcgctc cagggaatat taccggaaag
     1501 ccaccaaact ggatcccaac ctatcacagg cctggttggg aatcggcgtc acgt
//
LOCUS       DAOTON010000739         1553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_63299_length_1553_cov_3.201602, whole genome shotgun sequence.
ACCESSION   DAOTON010000739 DAOTON010000000
VERSION     DAOTON010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63299_length_1553_cov_3.201602"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="P5292_12265"
     CDS             <1..889
                     /locus_tag="P5292_12265"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HRS39945.1"
                     /translation="FWLLIALSHDYAATITFPVRYINLPDRKVVMNELPEKVSITLRT
                     SGFRILSYEFSSKRSPIEIDVASKLGGKGGRTDALALATRMFQPDFTRQLGTQVSLQA
                     YEPDSIVFYFSDQQTVRLPVQVPLTIKLARQFDTTGSLSIRPDSVTVTGPPSLIRHLK
                     GISTLPLDLRDLRSPVNTKLRLQENRLLTFSDSVVHVRLPVEELTEAKLEVPVRTINT
                     PATYILKTFPDKINLRLQVPLSHYREVQAADFDVVVDGARLLTDKPQRLAIKVISAPS
                     WARSILPEPGQVDYILRKE"
     gene            886..1497
                     /gene="coaE"
                     /locus_tag="P5292_12270"
     CDS             886..1497
                     /gene="coaE"
                     /locus_tag="P5292_12270"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585810.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRS39946.1"
                     /translation="MMIVGVTGGIGSGKTTVCKVFRALGIPVYDADQAAKELYDRDPE
                     LMRSVRDVFGDTILDPEGKLDRKKLAELVFADPEKLQVLNSLVHPRVRADFRKWVKLQ
                     THAPYVIREAAILFESGTHKDCDKVITVTAPVELRMQRVRQRDQRSEEEIRRMMERQW
                     SDEEKIRRSDAVLVNDERDLLVPRIVALHQEFLQLAQPNLSAQ"
BASE COUNT          368 a          415 c          434 g          336 t
ORIGIN      
        1 gttttggttg ctgatcgcct tgtcacacga ttatgcagcc accattactt ttcctgttcg
       61 ttatatcaat ctgcccgacc gtaaggtcgt catgaacgaa ttaccggaga aggtatcgat
      121 cacgcttcga accagtggct ttcggattct ttcgtacgaa ttcagcagta aacgttcacc
      181 cattgaaatc gatgtggctt ccaaactcgg aggcaaggga ggaagaacgg atgccctggc
      241 gctggcaacg cgtatgtttc aacccgattt cacccggcaa ctcgggacgc aggtcagctt
      301 gcaggcctat gaaccggatt cgatcgtgtt ctatttcagc gaccagcaaa ccgtgcgatt
      361 gccggtacag gtacccttga ccatcaagct ggccaggcag ttcgacacca caggatcgct
      421 atcgatccgc cccgattctg tcaccgtgac gggtccaccc agtctgatcc gtcacctgaa
      481 gggtatcagc acccttccgc ttgacctgcg cgatctcagg agtccggtca ataccaagct
      541 tcggttacag gagaatcgat tgttgacgtt cagcgattca gtcgttcatg tccgacttcc
      601 ggtggaagaa ttgacggaag cgaagctgga agttccggtg cgcacgatca atacgcccgc
      661 tacctatatc ctcaagacct ttccggacaa gatcaacctc cggcttcagg ttccgctcag
      721 ccattaccgg gaagtgcagg cagccgattt cgatgtggtg gtcgacggag cccgattact
      781 gacggataag ccccagcgat tagccatcaa ggtgatctca gcgccatcct gggcgcgatc
      841 gatcctgccg gagcccggcc aggtggacta tatactgcga aaggaatgat gatcgttggt
      901 gtcaccggtg gaatcggcag cggtaagacg actgtttgca aagtgtttcg cgcactgggc
      961 atacctgtct atgacgccga ccaggcggcg aaggaactct acgaccgtga cccggagctg
     1021 atgcgctccg tccgcgacgt gtttggagat actatcctcg atccggaagg gaaactcgac
     1081 cggaagaagc tggctgaact ggtattcgcg gatccggaga aattacaagt actcaactcg
     1141 ctggtacatc cccgtgtacg ggcggatttt cgaaaatggg tcaaacttca gacccatgcg
     1201 ccctatgtca tccgggaagc ggctatcctg ttcgaaagcg gtacccataa ggattgcgat
     1261 aaggtgatca cggtgacagc tcctgtcgag ctacggatgc aacgtgtaag acagcgtgac
     1321 caacgatcgg aggaggagat ccggcgcatg atggaacggc aatggagtga cgaagagaag
     1381 atacgtcgtt ccgatgcggt gctggtgaac gatgagcgcg atctgctggt gccccgcatc
     1441 gtcgccctgc atcaggagtt cctgcaactg gctcaaccca acttgtccgc acaataaagt
     1501 cgacgatgaa caggatcgga gcattgctgg aaaaaagaaa ggaaggttgg tat
//
LOCUS       DAOTON010000740         1550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_63488_length_1550_cov_2.432107, whole genome shotgun sequence.
ACCESSION   DAOTON010000740 DAOTON010000000
VERSION     DAOTON010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63488_length_1550_cov_2.432107"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="P5292_12275"
     CDS             complement(<1..794)
                     /locus_tag="P5292_12275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRS39947.1"
                     /translation="MTLRKILLGLVLLLSGLSASGQQDILVSQYMFNHLLLNPGYAGS
                     KDYMMASLLYRKQWVDFKGAPETQIASIHGPVGLTNLGWGVLLSHDKIGVTDRTDAYL
                     NAAYHLKAFGKSKLSLGLRAGGGYYSYKNSDLIVFDPGDPTFAGDRATRFLPNVGAGL
                     YLYGEKYYAGLSVPTIISYDPEKSLSLDLSSGEVVPRQVRHYFATVGTVLEVNPDVVL
                     KPSVLVKYVQNAPVEADFNLNVLLANVLWIGGSYRTQDAIVGLVEL"
     gene            complement(900..>1550)
                     /locus_tag="P5292_12280"
     CDS             complement(900..>1550)
                     /locus_tag="P5292_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS39948.1"
                     /translation="TFDNPVLSNAVATLIDYGSDTLNWTITNGACTTTDYLLITAAEP
                     VNAEAGDYQLVCANDAVLIARGFINGVGIWSVVTGSGNLADPTNDSTSVMDLPVGNTV
                     FLWTITNGPCSAADTVSVDYDNACELELPSGFSPNGDGKNDGYHIKGIEGYPDNVFRV
                     FNRWGNLVYDKEDYVNEDWVGQNNSGDPLPDGTYFVVLEVVGKNLRKSTYVDLRRQ"
BASE COUNT          348 a          426 c          408 g          368 t
ORIGIN      
        1 tgcaactcca ccaagcctac gatcgcgtcc tgcgtacggt agctgccgcc gatccaaagc
       61 acgtttgcca gcaggacatt caggttgaag tcggcttcca caggagcgtt ctgcacgtat
      121 ttaaccaata cgctgggctt caaaacgacg tccggattca cttccagtac cgttccaacc
      181 gtcgcgaagt agtggcgtac ctgacgcggc actacttccc cactgctgag gtccaggcta
      241 aggctttttt caggatcgta actgatgatg gtcgggacgc tcaggccggc atagtatttc
      301 tcaccgtaca gatagaggcc ggcaccgaca ttcggcagga agcgtgttgc tcgatcacct
      361 gcaaaggtcg ggtcacccgg atcgaaaacg atgaggtcac tgttcttata ggagtagtaa
      421 ccaccgccgg cacgtagtcc aagcgacagc ttactcttcc cgaacgcttt caaatggtag
      481 gcggcattga ggtaggcgtc cgtgcgatcg gtaacgccga tcttgtcgtg actgagtaac
      541 acaccccatc ccaggttggt caacccgacc gggccgtgaa tgctggcgat ctgggtctcg
      601 ggtgcgccct tgaagtcaac ccactgcttt cgataaagca ggctggccat catgtaatcc
      661 ttgctgccgg cataacccgg attgagcaac aggtggttga acatgtactg gctgacaagg
      721 atgtcctgct ggccggaggc agataagccg ctcaacagca gcaccaggcc taagagtatc
      781 ttgcgtagcg tcattgtcgc aatattcgtg tttttagttc gtgtaaagtc ttgtttttct
      841 cctcttgctc aaggaacaat caacagcggt cggtgaacag cgcaaaggat tttaatctct
      901 tattgtctgc gcagatcgac gtaggtgctc ttccgcaggt tcttcccaac tacttccagg
      961 acgacgaagt aagtgccgtc cggcagcggg tcgccgctat tgttttgtcc aacccaatct
     1021 tcgtttacgt aatcttcctt gtcatagacc aggttgcccc agcgattgaa cacccggaat
     1081 acgttgtccg gatagccttc gatacctttg atatgatacc cgtcgttctt tccatcacca
     1141 ttgggtgaga agccgctcgg aagttccaat tcacaggcat tgtcataatc cacgctaacg
     1201 gtatcggcag cagaacacgg accgttggtg atggtccaga gaaatacggt attaccgacc
     1261 ggcaggtcca tgacggatgt cgagtcgttc gtcggatcag cgagattgcc acttccggtt
     1321 acgaccgacc atattcctac tccattgatg aaaccgcggg cgatcagcac ggcatcattc
     1381 gcgcaaacga gctgatagtc tcccgcttcc gcattcaccg gctccgctgc cgtaatcagc
     1441 aggtagtccg tggtggtaca agcaccattg gtgatcgtcc agttcagggt atcagaaccg
     1501 taatcgatca gggtggctac ggcgttggac aatacgggat tgtcgaaggt
//
LOCUS       DAOTON010000741         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_63596_length_1548_cov_3.308774, whole genome shotgun sequence.
ACCESSION   DAOTON010000741 DAOTON010000000
VERSION     DAOTON010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63596_length_1548_cov_3.308774"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="P5292_12285"
     CDS             complement(<1..452)
                     /locus_tag="P5292_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS39949.1"
                     /translation="MGTESVDLLIQRAQVLRSRNVHQAMNLLDDALALAQQFDYKQGI
                     AQVIREKAACHLSQQHYKHALTAYSEALQFYRDLSDRTGQLSCLAELSNIYFKLGDCP
                     SSLQYVLDCLKIHTDAGDSEGIAQCYNEIGKIYIYLQDYPNAIEHFRKA"
     gene            complement(539..>1548)
                     /locus_tag="P5292_12290"
     CDS             complement(539..>1548)
                     /locus_tag="P5292_12290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS39950.1"
                     /translation="GKKFLEQFLPDVIRCSQPLGRVVIADNASTDDSLNWVRSHHPEV
                     EIIALPDNTGYTGGYNASLKQVQAEFYVLLNSDVEVTPGWLEPLVALADRHPTLGAAQ
                     PRIRAFNDRTHFEYAGAAGGFIDHLGYPFCRGRIFQSLERDEEQYADELPVFWATGAC
                     MFVRSKVFHQVGGLDDRFFAHMEEIDFCWRIQRAGFDVWVSPKSIVYHVGGGTLPKYN
                     PRKTYLNFRNNLLMLYKNLDFNEFQKVYRIRWWLDRLAAFQFLITSGQADAKAVLQAH
                     RDFLKLREQYPKSDQKPHWKGFPGVYPRSILLDHYLRGVKRFSALQRYFPANGRNSSH
                     L"
BASE COUNT          357 a          397 c          432 g          362 t
ORIGIN      
        1 gccttgcgga aatgttcgat ggcattcgga taatcctgca ggtagatgta gatctttccg
       61 atctcattgt agcactgcgc aattccttcg ctatcgccag cgtcggtgtg gatcttcagg
      121 cagtcgagta cgtattgcag cgacgaaggg caatcgccca gtttgaagta gatattcgaa
      181 agttccgcca gacaggacag ttgaccggta cggtccgaca gatcccgata gaactgcaag
      241 gcttccgaat aagccgtcaa tgcatgcttg tagtgttgct gggagaggtg gcaagccgcc
      301 ttttcccgga tgacttgcgc gattccctgc ttgtagtcga actgttgggc aagcgcaaga
      361 gcgtcatcca acaggttcat ggcctgatgg acgttccgcg agcgcaatac ttgtgcccgc
      421 tggatcaata gatcgacgct ttcggtaccc ataacaatcg tgtgctcaga gccactaacg
      481 gccagccctg attatagtgt atcctgggag gacggttgcc tgaatttgat cggtcgcctt
      541 acagatggct tgaatttcgg ccgttggcgg ggaaatatcg ttgcaatgct gaaaagcgtt
      601 tgacaccgcg aagatagtga tccagcagga tcgagcgtgg ataaacccct ggaaaccctt
      661 tccagtgagg cttttgatca ctcttcgggt attgttcacg gagtttcaaa aagtcacggt
      721 gggcttgtaa aacagccttc gcgtcggctt ggccgctggt gatgagaaac tgaaaggcgg
      781 ccaggcggtc cagccaccag cgaattcggt aaactttttg gaattcgttg aaatccaggt
      841 ttttataaag cattaagagg ttattcctga aattgaggta ggttttccgt ggattgtact
      901 tcggaagcgt gccgccaccc acgtgataca cgatactttt cgggctgacc catacatcaa
      961 aacccgcccg ttggatcctc caacagaagt cgatctcttc catgtgcgcg aagaaacgat
     1021 catcaagtcc gccaacctgg tgaaagacct tggagcgaac gaacatgcag gcgccggttg
     1081 cccagaagac cggtagttca tcagcatatt gctcttcgtc ccgttccagc gattgaaaga
     1141 tccggcctcg gcaaaacgga tagccgagat gatcgatgaa tccacctgcg gcaccggcgt
     1201 attcgaaatg cgtccggtcg ttgaatgcgc ggatccgggg ttgggccgca cccaaggtgg
     1261 ggtgccgatc agcgagcgct accaggggtt ccagccatcc gggggtaact tccacatcgg
     1321 aattaagcaa cacgtagaat tcggcctgca cctgcttcag cgaagcattg tatccaccgg
     1381 tgtagccggt gttatccggc agtgcgatga tttccacttc gggatgatgg ctgcgtaccc
     1441 agttcagcga atcatcggta gaggcgttat cggcaatcac cacgcgcccc aggggttggc
     1501 tgcaacggat cacgtccggc agaaactgct ccaggaattt cttgccat
//
LOCUS       DAOTON010000742         1547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_63647_length_1547_cov_4.692359, whole genome shotgun sequence.
ACCESSION   DAOTON010000742 DAOTON010000000
VERSION     DAOTON010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63647_length_1547_cov_4.692359"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="P5292_12295"
     CDS             complement(<1..247)
                     /locus_tag="P5292_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRS39951.1"
                     /translation="METLNACPICQSGSFLPELVAKDYTVSGKEFQIVKCAECGLLFT
                     NPRPTKLEIGPYYESPDYISHSNSKKGLVNNIYQWVRN"
     gene            complement(272..>1547)
                     /gene="mnmG"
                     /locus_tag="P5292_12300"
     CDS             complement(272..>1547)
                     /gene="mnmG"
                     /locus_tag="P5292_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRS39952.1"
                     /translation="PRLDGRSIDYSKTEEQPGDTIPGRFSYLDAPLPDRQLSCWITYT
                     NDAVHDILKEGFDQSPMFAGRIQGLGPRYCPSIEDKINRFADKERHQLFIEPEGWDTV
                     EIYLNGFSTSLPEEVQYKALRQVPGLENVKMFRPGYAIEYDYFPPTQLNLTLETKGIE
                     NLYFAGQINGTTGYEEAGCQGLMAGINAHRKINGLEPVVLKRSEAYIGVLIDDLVTKG
                     TDEPYRMFTSRAEFRTLLRQDNADIRLTPLGYSIGLAKEERMRRVERKMLETDRILGF
                     LRQESAFPDQVNPMLEERNTSAISQKQKLFNLLSRPQLGMQDLLGSVDSLTSFAAGIE
                     EDFRAMALEQAEIMVKYEGYIEKEQEIVEKFARLEEINLHDEFDYESLSALSMEARIK
                     LSKIRPRTLGQASRISGVSPSDISVLMVHLGR"
BASE COUNT          358 a          376 c          393 g          420 t
ORIGIN      
        1 gatttcgtac ccattggtag atattgttga ctaggccttt tttgctgttg gaatgactga
       61 tatagtctgg gctctcgtag tatggcccaa tctcgagctt cgttggacga ggattcgtaa
      121 agagcagtcc gcattccgcg cacttgacga tttggaattc ttttccggat acggtgtagt
      181 cctttgcgac taattcaggt aaaaaagaac ccgattggca gatcgggcag gcgtttaggg
      241 tttccataaa gaatatgttc cacgtgaaac atcagcggcc caaatggacc atcaggaccg
      301 aaatatcaga cggcgataca ccgctgatcc tggaggcctg acccagtgtg cgagggcgga
      361 tcttcgagag tttgattcgg gcctccattg agagtgcgct cagggattcg tagtcaaact
      421 cgtcgtgtag attgatttcc tccaaacgcg cgaacttttc tacgatttcc tgctcttttt
      481 caatataacc ctcatatttc accattattt cggcctgctc cagtgccatt gccctaaaat
      541 cctcttcgat tccggctgca aaggaagtga ggctatcaac cgatcccaaa aggtcctgca
      601 tccccaattg aggtctggaa agcaggttga agagcttttg cttttgagaa atagcggaag
      661 tgttacgctc ttccagcatt ggattcactt gatccgggaa agcggattct tgtcttaaaa
      721 agcccaaaat ccgatcagtt tcgagcatct tgcgctctac cctgcgcatt cgttcttctt
      781 ttgccagacc gatggaatat cccaacgggg ttagtcggat atcagcatta tcctgtcgca
      841 acaaggtcct gaattcggcc ctggaagtaa acatccgata aggctcatcg gtgccctttg
      901 tgaccaggtc gtcgatcagc acaccgatat aggcctcgga acgcttgagg acgaccggtt
      961 cgagtccgtt gatcttgcga tgtgcgttaa tgccggccat caggccctgg cagccggctt
     1021 cttcgtaacc ggttgttccg ttgatttgtc cggcgaaata gagattctca atacccttgg
     1081 tttcgagcgt caaattcaac tgtgtgggcg ggaagtagtc gtattcaata gcgtagcccg
     1141 gacggaacat tttgacgttc tccaaaccgg gtacctggcg aagcgctttg tactgtactt
     1201 cttccggtaa tgaggtcgaa aatccgttca agtagatctc cacggtgtcc caaccttccg
     1261 gctcgataaa caactgatgc cgttctttat cggcgaagcg gttgatcttg tcttcgatcg
     1321 aaggacaata acgcggacca agtccctgaa ttcgtccggc gaacatgggg gattgatcga
     1381 atccctcttt caggatatcg tgtactgcgt cattagtgta ggtaatccag caactaagtt
     1441 gacgatcggg tagtggtgca tccaggtaag aaaagcgccc gggaatggtg tcgcctggtt
     1501 gttcttctgt tttcgaataa tcgatcgaac gaccatcgag tcgggga
//
LOCUS       DAOTON010000743         1546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_63723_length_1546_cov_3.718310, whole genome shotgun sequence.
ACCESSION   DAOTON010000743 DAOTON010000000
VERSION     DAOTON010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63723_length_1546_cov_3.718310"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="P5292_12305"
     CDS             complement(<1..537)
                     /locus_tag="P5292_12305"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRS39953.1"
                     /translation="MSTKRRRTILRWMWAGVFVLLLVIGGIGYVAYERIYQPNVEVAD
                     PNRNFLLIPTGADFDDVINLLHEKKALKDEASFRWTAGRMKYGSDVKPGRYKLRSGMS
                     NRELVQLLRSGRQTPVKLVFNNIRTTRELADRIGQQLELEPDALLHLLEDNDYLQPLG
                     FNAGNVLAMFLPDTYEFYW"
     gene            641..1006
                     /locus_tag="P5292_12310"
     CDS             641..1006
                     /locus_tag="P5292_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39954.1"
                     /translation="MNLRIYHLERAALHSRCKSVFVTLGYQLIEEDPLNCILRAVREE
                     PAGACSSLLDIRIKKEPDGIQVLVLSGQRSNVFGTIRFDPDGETEFIEHFLFAIQPAV
                     IRHPEFRLTEADYAFASGF"
     gene            1091..1462
                     /locus_tag="P5292_12315"
     CDS             1091..1462
                     /locus_tag="P5292_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS39955.1"
                     /translation="MNKPLKVLVVDDEPDVVEILSYNLSRENYLVYKAYNGSDGVRVA
                     HEVHPDLVIMDIRMPGINGIEACRQMKQDDETKTIPVLFLTADADEYTTMNAIEAGGD
                     HFITKPIRPQILMGMIAELIR"
BASE COUNT          380 a          426 c          362 g          378 t
ORIGIN      
        1 ccaataaaac tcgtaggtat ccggcaggaa catggccagc acattccctg cattgaaacc
       61 cagtggctgg aggtagtcat tatcttccag cagatgcagc agcgcatccg gttccagttc
      121 caattgctgc ccgatccgat ccgccaactc gcgcgtagtc cggatgttgt tgaaaaccaa
      181 cttgaccggt gtctgccgtc cggatcgaag gagttgaacc agttcccggt tactcatccc
      241 tgaccggagt ttatatcgtc cgggctttac gtctgatccg tatttcatcc tcccggcggt
      301 ccaccggaat gaagcttcgt ctttcagcgc ctttttctcg tgcagtaggt tgatcacatc
      361 atcgaaatcg gctcccgtag ggatcaacag gaaattccgg ttggggtcgg ccacttcaac
      421 attgggctga tagatccttt cgtaggccac atagccgatt ccaccgatca ccaacaataa
      481 cacgaatacc cctgcccaca tccagcggag tatggtcctt cttctcttag tactcatctt
      541 gctctagcta aggtagccag gaggcttatc ggaagcgtaa cagtccgtta attttttctc
      601 aacacgacca gccttatttc accatcaaat tcatatgacc atgaacctcc gaatctatca
      661 cctggaaaga gcagccctgc attcccgttg caaatcggtc tttgtcaccc tcggctacca
      721 gctaatcgaa gaggatccac tcaactgtat cctgcgagcc gtgcgtgagg agcccgcagg
      781 ggcctgttcg agtctgcttg atatccggat aaaaaaagag ccggatggaa ttcaagtgct
      841 ggtcctcagc ggacagcgat cgaatgtatt cgggaccatc cggtttgatc cggatgggga
      901 aacggaattc atcgaacatt ttttgtttgc catccagccc gccgtaatcc gtcatcccga
      961 atttcggcta acagaagccg attatgcgtt tgcatccggt ttttgagcca atggtcttaa
     1021 caattaattc accccggatt aatcgcgggt ttacgttggg tggacatctt tgaatcgata
     1081 aaccgaacaa atgaacaagc ccttaaaagt cctggtggta gatgatgaac cggatgtagt
     1141 cgaaatcctg agctacaatc tttccaggga gaactacctg gtctataagg cctacaatgg
     1201 cagtgatggg gttcgtgtcg ctcacgaagt tcatcccgac ctcgtgatca tggatatccg
     1261 gatgccggga atcaatggca ttgaagcctg ccgtcagatg aagcaggacg atgaaaccaa
     1321 gaccattccc gtactatttc tcacggccga tgcggatgaa tacaccacca tgaacgccat
     1381 cgaggccggt ggtgaccact ttatcaccaa accgattcgt ccacagatcc tgatgggcat
     1441 gatcgcggaa ctaattcgat gaccatgatg ttcatgtcct acgcacccgc agcaccttta
     1501 cttctagtag ccgaatccac cgccgaacac cgccagcttg cagaat
//
LOCUS       DAOTON010000744         1546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_63742_length_1546_cov_2.477532, whole genome shotgun sequence.
ACCESSION   DAOTON010000744 DAOTON010000000
VERSION     DAOTON010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63742_length_1546_cov_2.477532"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="P5292_12320"
     CDS             complement(<1..1017)
                     /locus_tag="P5292_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRS39956.1"
                     /translation="MSEHSRSGISRLAASALMPQEAMVEVHKQEKKLLIGIPKETSFQ
                     ENRIPLVPEAVAQLTANGHEIIIEAGAGAASNFQDSDFSEAGARIVYSVEEVFQCDVV
                     LKVAPPSEKELDLLKTRQTLVSILQMSMQNEQYVRKLSSKKITAIGYEFMHDDSGVYP
                     IIQAMSEIVGSACILIAAEYMSNAFNGKGELLGGVAGIPPTEVVIIGAGTVGEYAARA
                     ALGLGATVKVFDNSVQRLRRIQNHLGNRIFTSTIVPSILLKELKHADVAIGALRSSEG
                     RTPVVVTDEMVSEMQVGSVIVDVSIDQGGCFETSEVTNHSQPVFRKYGVVHYCVPNIA
                     SRVSR"
     gene            complement(1010..1426)
                     /gene="tsaE"
                     /locus_tag="P5292_12325"
     CDS             complement(1010..1426)
                     /gene="tsaE"
                     /locus_tag="P5292_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRS39957.1"
                     /translation="MVLEGISLDRLGEVADALIRYAGDVRVLAFYGEMGAGKTTFIKS
                     LCTGLGVHGPVTSPTFSIVNEYPGPSGNRIYHFDFYRLKQEREAVEIGLEEYLDSGQW
                     CLLEWPEKVLNLLPNPRIDVRITVVDGYRRFEFSHV"
BASE COUNT          362 a          437 c          378 g          369 t
ORIGIN      
        1 tctgctgaca cgggaagcta tgttcggtac gcaatagtgg acgacaccat atttccggaa
       61 aacaggctgc gaatggttcg tcacttcgga agtttcaaaa caaccgccct ggtcgatact
      121 cacgtcgaca atgacggaac ccacctgcat ttcgctgacc atttcatcgg tcactaccac
      181 gggagtacgt ccttcgctgc tgcgtaatgc gccaatggcg acatcggcat gcttgagctc
      241 tttgagcagg atgctgggca ctatggtcga tgtgaagatg cgattcccga ggtgattctg
      301 gatccgccgg agtcgctgca cactgttgtc aaacactttt acggttgctc caagacccaa
      361 ggcggcccgg gcagcatatt cgccaacggt tcccgcaccg atgatgacca cttcggtagg
      421 cgggataccg gccacgccac ccagcaattc gcccttccca ttgaaagcgt tgctcatgta
      481 ttcagctgcg atcaggatgc aggcactgcc gacgatttcg ctcatcgcct ggatgatcgg
      541 gtaaactccg gaatcgtcgt gcatgaactc ataaccgatg gccgtgatct ttttcgaaga
      601 aagcttgcga acatattgtt cgttctgcat gctcatctga agaatggata cgagcgtctg
      661 acgcgttttg agaagatcca attccttttc agatggcgga gcgactttca aaacgacgtc
      721 gcactggaag acctcttcca cggagtaaac aatgcgcgcg cctgcttcgc taaagtcgga
      781 atcctggaaa ttggatgcgg cgcccgctcc ggcttcgatg atgatctcgt gtccgttcgc
      841 ggttagctga gcaacggctt cgggcaccag cggaatgcga ttctcctgaa aggaagtttc
      901 ttttggaatg ccgatgagca gtttcttctc ctgcttgtgc acctccacca tcgcctcctg
      961 gggcatcagc gcggaggctg caagacgcga tattccggaa cgtgaatgtt cagacatggg
     1021 aaaactcgaa tcgtcgataa ccgtcgacaa ccgtgattcg gacgtcgatc cttggatttg
     1081 gcaataaatt tagcactttt tccggccatt ccagcagaca ccattggcca ctatcaaggt
     1141 attcctccaa accgatctcg actgcttctc gctcctgctt cagacggtag aaatcgaaat
     1201 gatagatcct attcccggaa ggtccggggt actcgttgac gatggaaaag gtcggactgg
     1261 tcacgggtcc atgaactccc aaacccgtgc aaagactttt aataaaggtc gtcttcccgg
     1321 ctcccatttc accgtaaaaa gcaagcaccc gaacatcacc ggcatagcgg atcaatgcat
     1381 cggcgacttc tcccaggcgg tccaggctta tgccttccaa caccatgctt ctatacctta
     1441 atgattcgtt tccaatccgt cacttgcaac accctgtcac aaaaggaata ttcctcggca
     1501 atgttattgg aacaaaccac caggatccgc cctgctccat gctcct
//
LOCUS       DAOTON010000745         1545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_63798_length_1545_cov_2.656376, whole genome shotgun sequence.
ACCESSION   DAOTON010000745 DAOTON010000000
VERSION     DAOTON010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63798_length_1545_cov_2.656376"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..>1545
                     /locus_tag="P5292_12330"
     CDS             165..>1545
                     /locus_tag="P5292_12330"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRS39958.1"
                     /translation="MSATLILTCVVLYSALLFFVVWLTSRGADNESYFIGNRSSKWYV
                     VAYGMIGASLSGVTFMSVPGWVGTTGMSYMMVVFGYLLGYAAIAGILLPVYYRMRLTS
                     IYSYLEQRFGFWSYKTGAFYFILSRTIGASFRLYIVVNVLQTFVFDAWGVPFAVTVGV
                     FILLILLYTYKGGVKTIVWTDTLQTTFMLLSLVLSIVLILGKLGWSWGELVNQLTSGP
                     YSNMVVSDVKASNFAWKHFFGGMFIAIAMTGLDQEMMQKNISVRTLGESQKNMISFSL
                     ILVLVNLLFLSLGILLYNYVSVEGIATQFHTSDDLFPTVALQHLGTLSAVFFIIGLIS
                     AAYPSADGALTALTASFCIDFLGMERKDWSKEKKERTRYTVHIAFAILLLLVIVVFRI
                     INNDAVIGILFKAAGYTYGPLLGLYAFGLFTRHPVRDRFVPLVCLVAPVLCFWFNENA
                     PVWWNGYKFG"
BASE COUNT          310 a          407 c          394 g          434 t
ORIGIN      
        1 cgccgatcgc tttccgattc cgggtaagcg ggagaattcc tgtacagctt cttcgaggat
       61 tcgggatggg agactgctca cggaaggtgt tgaaaaaagg acactggtgc gggaatcccg
      121 ccgagacgaa attacttact tcggccagta ttcacaacca tagaatgtct gcaaccctta
      181 tcctgacctg tgttgtcctt tattcggcgc tgctcttttt cgtcgtttgg ctgacgagtc
      241 gtggtgccga taacgagtcc tacttcatcg gtaaccgatc ctccaagtgg tatgtcgtgg
      301 cctatggtat gatcggagcc tccttgtccg gagtcacgtt catgtcggtg cctggctggg
      361 tcggaaccac cggcatgtcg tatatgatgg tagtattcgg ttacctgctc ggttatgccg
      421 cgatcgccgg catcctgttg ccggtgtatt atcggatgcg gctgacatcg atctactctt
      481 acctggaaca gcgtttcggc ttttggagct ataagaccgg cgcgttttac tttatcctgt
      541 cccggaccat cggcgcttcc ttccgcttat acatcgtcgt aaacgtcttg caaacctttg
      601 ttttcgatgc atggggtgtc ccattcgcgg ttaccgtagg cgtattcatt ctcctgatcc
      661 tgctttatac ctacaaaggc ggcgttaaga cgatcgtttg gacggatacc ctgcaaacaa
      721 ccttcatgct cctgtccctg gtgctttcga tcgtcctgat actcggtaaa ctcggctggt
      781 cgtggggaga attggtgaac caactcactt cggggcccta ctcgaatatg gtcgtttccg
      841 atgtgaaagc ctccaacttc gcctggaaac atttcttcgg cggaatgttc atcgccatcg
      901 ccatgaccgg cctcgaccag gagatgatgc aaaagaacat cagcgtccgg acgctgggcg
      961 agtcgcaaaa aaacatgatc agtttcagcc taatcctggt gctggttaac ttgctctttc
     1021 tctccttggg cattctgctg tacaattacg tttccgtaga aggtatcgct acccaattcc
     1081 atacttccga tgacttattc ccaacggtgg cattacagca cctgggaacg ctttccgccg
     1141 tattctttat catcggactt atctcggccg cctatcccag cgcagatggg gctttgaccg
     1201 cactgaccgc ttcattttgc attgacttcc tgggaatgga acgaaaagac tggtcgaaag
     1261 agaaaaagga acggacccgt tacacggtac acattgcatt cgcgatactt ctgctcctgg
     1321 ttatcgtggt cttccggatc atcaacaacg atgcggtgat cgggatccta ttcaaagcgg
     1381 ccggctatac ctatggtccc ttgttgggat tgtacgcatt cgggctgttt acacgacatc
     1441 cggtacggga tcgcttcgtg ccgctggttt gtctcgtagc cccggtcttg tgcttttggt
     1501 ttaatgaaaa tgctccggtc tggtggaatg gctacaaatt cggct
//
LOCUS       DAOTON010000746         1544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_63849_length_1544_cov_2.750168, whole genome shotgun sequence.
ACCESSION   DAOTON010000746 DAOTON010000000
VERSION     DAOTON010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63849_length_1544_cov_2.750168"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="P5292_12335"
     CDS             <1..555
                     /locus_tag="P5292_12335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39959.1"
                     /translation="IIQQDGYNQYLLESEYVTAIAIDGANRKWFGTYSGGAFLMSADG
                     TKQLLNFNTGNSPLASNTILSIAVDGVTGEVFFGTDKGIVSYRGDATEGGEACSDYNV
                     FPNPVRHDYSGPIAVSGLVADADVRITDVAGNVVFHTKALGGQAVWNGNDFSGRRAQT
                     GVYLVYVTNEDGSATCVTRLLLAN"
     gene            555..1280
                     /gene="recO"
                     /locus_tag="P5292_12340"
     CDS             555..1280
                     /gene="recO"
                     /locus_tag="P5292_12340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRS39960.1"
                     /translation="MLEKTRGIVLRTTDYGETSLVVKVFTEAYGLQSYIVNSVRKPGA
                     KTRASVFQPLSLLELVVYHKDRGGLQRASEIRNFPPYRHLPFDMQKSSIVLFLDEVLN
                     KVLQEGGEQQEVFEFLVSSLQLLDLQQPANPDFHLAFLLRLTRLLGFFPAGAPSEATP
                     VFDLQEGVFCSQVPPHPHYLTGEECTAFGSLIEATNDFSKHLQWPLPLKRVMTERLLD
                     YYRLHVPGFGEVRGHKVLEAVWS"
BASE COUNT          310 a          411 c          463 g          360 t
ORIGIN      
        1 atcatccagc aggacggcta caaccagtac ctgctcgaat cggagtacgt gaccgcgatc
       61 gcgatcgacg gggccaaccg caagtggttc ggcacttaca gcggtggcgc gttcctgatg
      121 tcggcggacg gcactaagca actgctcaac ttcaacacgg gtaactcccc gcttgcttcg
      181 aacaccatcc tctcgatcgc ggtggacggc gtgaccggtg aagtattctt cgggaccgac
      241 aaaggcatcg tcagctatcg cggcgacgcg acggaaggcg gcgaagcctg cagtgactac
      301 aacgtcttcc cgaatcccgt gcggcacgac tacagcggac ccatcgcggt ctccggcctg
      361 gtggctgatg ccgacgtccg catcaccgat gtggccggca acgtggtgtt tcacaccaag
      421 gccctgggcg gacaggctgt ctggaacggc aacgatttct ccggacgccg cgcgcagacg
      481 ggcgtttacc tggtgtacgt gacgaacgaa gacggctccg cgacctgcgt gacgcgtctg
      541 ttgctggcga actgatgctg gaaaagaccc ggggcatcgt gctgcgcacc accgattacg
      601 gggaaacgag cctcgtggtg aaagtgttta ccgaagcgta cgggctgcaa tcctacatcg
      661 tcaacagcgt acgcaagccg ggcgcgaaga cgcgcgcttc ggtgttccag ccgctgtcgc
      721 tgctcgagct ggtggtgtac cacaaagacc gaggaggttt gcagcgggct tccgagatcc
      781 ggaactttcc gccttaccgt catctgccgt tcgacatgca gaagagttcc atcgtgctct
      841 tcctcgacga agtcctgaac aaagtgttgc aggaaggcgg cgaacagcag gaagttttcg
      901 agttcctggt gagttcgctg cagttgctcg acctgcagca gccggccaat cccgattttc
      961 atctcgcgtt cctgttgcgc ctgacgcgcc tgttgggatt cttccccgcg ggcgcgcctt
     1021 cggaggcgac tccggtgttc gatctgcagg aaggcgtgtt ctgttcacag gttccgccgc
     1081 atccgcatta tctgacggga gaggaatgca cggccttcgg atcgttgatc gaagcgacga
     1141 acgatttttc gaaacacctc caatggccct taccgctgaa gcgggtgatg accgaacgcc
     1201 tgctcgatta ctatcgcctg cacgtgccgg gtttcggcga ggtgcggggg cataaggtgt
     1261 tggaggcggt ttggagttaa ggtgcgaggt gcgaggtgcg aggtgcgagg tgcgagggaa
     1321 ttttgtttgt ttttggatta taaattcatt ttgtaaagtt atttgtttac tttattttga
     1381 tttttgtaat gttattgctt tacttttatg attgttttgt aaaactttaa ctttactttt
     1441 atagtattga acgattccag acctgacatc atggccagac gatctttatt gttgcagcag
     1501 ttagaccgga agatggaagc gtatgcaccg ctgaaacagt cggc
//
LOCUS       DAOTON010000747         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_64093_length_1540_cov_2.970370, whole genome shotgun sequence.
ACCESSION   DAOTON010000747 DAOTON010000000
VERSION     DAOTON010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64093_length_1540_cov_2.970370"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="P5292_12345"
     CDS             <1..819
                     /locus_tag="P5292_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39961.1"
                     /translation="DYRFDKPEQSVVLPPVLHEISGITSITDELLACVQDERGVIFFY
                     DLGKQQLVSNLRFHADGDYEGICRVGRDLYVLRSDGHLFRVRDFMLPKGNSGYTTVEY
                     PTGIIGKNNEGLCWDSIGNRLLIACKGKVGKGPEWKDQRHIYAFDLKTHQLDKDPLFA
                     FDLEDIRRFAVEHRVPLPTREKKKDGSSEPFLRFTTSAIAIHPDSHELYVVSSTDHLL
                     FVFGMDGKLHHLEVLDPVRFNKAEGIIFLSDGDLLITNEGQDKRPTLLRFDRKQ"
     gene            complement(852..>1540)
                     /locus_tag="P5292_12350"
     CDS             complement(852..>1540)
                     /locus_tag="P5292_12350"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRS39962.1"
                     /translation="FMKAVIEACSRHNARLVFFDNMYLYAASAVPHMTEEAAIDPPSE
                     KGKVRAQVHRMIFEAVEAGRLQALIARSADFYGPENKASALQIMVADNFRKGKKAQAF
                     GNVDKLHTYTFTPDAARATALLGNTADAFNQVWHVPTTRQRWTTRQWVECIAREMQVE
                     PRIQAVPRWIIKSLGLFMPIMREFPEMLYQYEQDYVFDSSKFERTFGWGATDPEAGVK
                     AMVRALTAHA"
BASE COUNT          332 a          451 c          414 g          343 t
ORIGIN      
        1 gattatcggt ttgataagcc tgaacaatcg gtcgtgttac ctccggtatt gcacgagatc
       61 tccggcatca cctcgatcac cgacgagctc ctggcctgcg tgcaggatga acgcggggtc
      121 atttttttct acgatctcgg taagcagcaa cttgtttcga atctgcgctt tcatgccgac
      181 ggcgactatg aaggcatctg tcgggtcgga cgggatctgt atgtgctgcg gagcgacgga
      241 cacctgtttc gcgtacgcga tttcatgctc ccgaaaggga attccggata tactaccgtc
      301 gaatatccta ccggtatcat cggtaagaac aacgaaggcc tctgttggga ctccatcggc
      361 aaccggctgt tgatagcctg caaaggaaag gtggggaagg ggccggaatg gaaggaccag
      421 cggcacatct acgccttcga cctgaaaacg catcaactgg acaaggaccc gctcttcgca
      481 tttgacctgg aggatatccg gcgctttgcc gtcgaacacc gcgttccgct gccaacacgc
      541 gagaagaaaa aagacggcag cagtgaaccc ttcttacgct tcacgacttc ggccattgcc
      601 attcacccgg actcccacga attgtatgtc gtatcgtcga ctgatcactt gctgtttgtt
      661 ttcggcatgg acggcaaact ccatcacctg gaagtgttgg atcccgtccg attcaataaa
      721 gcggaaggaa tcatcttttt atccgacggc gacctgctca tcaccaacga gggtcaggat
      781 aagcggccca ccctgttgcg cttcgatcga aagcaatagc ctcgcctgga gggaagaagc
      841 catacgcact cctaagcgtg ggcggtaagc gccctgacca tcgcctttac acctgcttcc
      901 ggatcggtcg cgccccagcc gaacgttcgc tcgaacttac tactgtcgaa gacataatcc
      961 tgctcatact gatagagcat ttccggaaac tcccgcatga tcggcatgaa cagtcccagg
     1021 gacttgatta tccagcgcgg caccgcctgg atacggggct ccacctgcat ctcccgtgcg
     1081 atgcattcga cccactgacg ggtggtccaa cgctgccggg tggtgggtac atgccatacc
     1141 tgattgaacg catcggcggt gttacccagt aaagcggtgg ccctggccgc atccggggtg
     1201 aacgtgtagg tgtgcagctt gtcgacgttg ccaaaggcct gtgccttctt accctttcga
     1261 aagttatcgg ccaccatgat ctgcaaggca ctggccttgt tctccggtcc gtaaaaatcg
     1321 gccgagcgtg cgatcagcgc ctgcaaacgt ccggcttcca ccgcttcgaa gatcatgcgg
     1381 tgtacctgcg cccgcacttt ccctttctcg ctgggcggat cgatcgctgc ctcttccgtc
     1441 atgtgcggaa ccgctgaggc cgcgtacaaa tacatattgt cgaaaaagac cagccgggcg
     1501 ttgtgtcgcg aacaagcttc gatcaccgcc ttcatgaagg
//
LOCUS       DAOTON010000748         1539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_64172_length_1539_cov_2.728437, whole genome shotgun sequence.
ACCESSION   DAOTON010000748 DAOTON010000000
VERSION     DAOTON010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64172_length_1539_cov_2.728437"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..796
                     /locus_tag="P5292_12355"
     CDS             41..796
                     /locus_tag="P5292_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39963.1"
                     /translation="MNITNRKGYDNQPSFIPGTSTLLYVRIPDSGSADIRQYELSGKD
                     VAITDSPESEYSPSYSHNMQFLSMVRVDLDSGQRFYRLPTAHHDISIPRPEPGTDSIG
                     YYCWMNDSMLAMFILGPANTLQVLNLRTHQRTLIASDIGRCMRMNPDRSELYFVVKAN
                     ELEWKILAMNAKTYATRELFPTLAGSEDYTVLSDGSLLMGSKGKLYRRKLNSGSEWAE
                     IADFSKSIGDFYRIAVNSASDRLALVGFTGTRP"
     gene            793..>1539
                     /locus_tag="P5292_12360"
     CDS             793..>1539
                     /locus_tag="P5292_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS39964.1"
                     /translation="MNQDAVVDYLIVGQGLAGTLLGRAFERRGISFRIVEQEKPVSSS
                     RVAAGLVHPVTGRRRAKSWMADTFLTFNESYYNSLHRDTGELFYHAVDGLEVAATVKE
                     CNDWTARMSDPDLDHLLVGQYTDDDYKTALQPSAGQFVMRHCGWMDLPRFLDFFRVKW
                     SNQGILLTDTFDPTSLQLTEEFVQFKQVSARSIIFCEGEQARKNPLWSWLPFDPVKGE
                     ILTIRVPGLPQQHIIFRGLFLVPIGNERFRA"
BASE COUNT          359 a          409 c          383 g          388 t
ORIGIN      
        1 cattatcact gtgacccttg acctttatcc atttaatcct gtgaacatca caaaccggaa
       61 aggatacgac aaccagccca gcttcatacc gggtacttct accctgttgt atgtgcgcat
      121 tccggattcc ggatctgccg atatccgtca gtacgaatta tccggcaagg atgtagcgat
      181 aaccgattcg cccgaaagcg aatattcccc ctcctattcg cataacatgc aattcttgtc
      241 gatggtacgc gttgacctgg attccggcca acggttctac cgtctgccta ccgcccatca
      301 tgacatttcc ataccccgtc ctgaacccgg caccgattcg atcggatact attgctggat
      361 gaatgacagc atgctggcca tgttcatcct gggtccggcc aatacgctgc aagtgctgaa
      421 cctccgaacg catcagcgta ctctcatcgc ctctgacata ggacgttgca tgcgcatgaa
      481 tccggatcgc tcggaactgt attttgtcgt caaggcaaac gagcttgaat ggaaaatact
      541 tgccatgaac gcgaaaacct atgcaacaag ggagcttttc cccacgctgg ccggcagcga
      601 agattatact gttctatccg atggcagttt gttgatggga tccaaaggca agttgtacag
      661 acgaaagttg aatagcggaa gcgaatgggc cgagatcgcc gacttttcca aatccatcgg
      721 cgacttctat cggattgccg ttaactcggc atcggatcgt cttgctctgg tcggatttac
      781 cggtacgcgt ccatgaatca ggatgcggtg gtagattatc tgatcgtcgg tcagggattg
      841 gcaggtacac tactggggcg cgcttttgaa cgccggggaa tctccttccg cattgtcgaa
      901 caggaaaaac cggtatcctc ttcacgcgtt gcggcgggtt tggtccatcc ggtaaccggt
      961 cgcaggcgtg ctaaaagctg gatggccgac acctttctca ccttcaatga atcctactat
     1021 aattccctgc atcgcgatac cggcgagttg ttctatcacg cggtcgatgg actggaagta
     1081 gccgccactg taaaggaatg caacgactgg actgcccgca tgtccgatcc ggatctcgat
     1141 cacttgctcg tcgggcagta tacggatgat gactataaaa ctgccttaca accttcggcg
     1201 gggcaattcg tcatgcggca ttgtggttgg atggatctgc cccgttttct tgacttcttc
     1261 cgggtaaaat ggagtaacca agggatatta ttgacggaca cctttgatcc gacatcactt
     1321 caacttaccg aggagttcgt tcaattcaaa caggtcagcg cgcggtccat catcttctgt
     1381 gaaggggaac aagcgagaaa gaatccatta tggtcctggt taccatttga ccctgtgaag
     1441 ggagagatcc tcacgattcg cgtaccgggt ttgccgcaac agcatattat tttccgtgga
     1501 ctattcctcg tgccgattgg aaatgagcgc tttcgagcc
//
LOCUS       DAOTON010000749         1535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_64395_length_1535_cov_3.894595, whole genome shotgun sequence.
ACCESSION   DAOTON010000749 DAOTON010000000
VERSION     DAOTON010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64395_length_1535_cov_3.894595"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="P5292_12365"
     CDS             <1..983
                     /locus_tag="P5292_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HRS39965.1"
                     /translation="SNITSFGQIGGGAINPNGTWSLRVYDRAGGDVGTINSWILHLPA
                     QTNTCTSLSNSIIVTELPAVTVSGFSPTAGTAGTLVTISGTGFTGATAVTFNGTSASF
                     TVVNSTTITATVPAGATTGVVAVTAPCGTASSGTAFTIGSSTTLNVHVLIEGYHLGGG
                     VMPEVSATGVCDTVTVVLAAAASPGVDFATARAAINTSGWGAFVFSGLTPGSYYIGVR
                     HRNSIETWSASAISLSAGSATYDFTTASTQAYGGNLRNLGGGRFALFSGDVNQDDLIE
                     SADYGAVENAVVGFLSGYQVEDLTGDNLVESTDYSLIENNLMGFIFSIRP"
     gene            complement(995..>1535)
                     /locus_tag="P5292_12370"
     CDS             complement(995..>1535)
                     /locus_tag="P5292_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS39966.1"
                     /translation="LSSNSVLVNANAGKSLLDESQKEGIKGTPQEQAYIEEGIRYLQR
                     SVDIYPKFPKGYSNMGVAYFYQGKYPEAVAAWLKAKEYHHNDPALRTYAEVLMNNALM
                     KGSNKDFAGAIQWLEWARQLDDRNENILYNLGGAYFSGGQLEKARSTWEFLLTNINPN
                     NEKAKQGYAAASQLIGQQR"
BASE COUNT          316 a          406 c          420 g          393 t
ORIGIN      
        1 cctcgaacat tacgtctttt ggacaaatcg gtggaggtgc gatcaatccg aatggtacct
       61 ggagcctacg ggtctacgac agagccggtg gagatgtggg tacgatcaat tcctggatct
      121 tgcacctgcc tgcacagact aatacctgca cgagtttgtc caattcaatt atcgttactg
      181 agttgcctgc ggtcacggtc tccggttttt cgccgactgc aggaactgca ggaaccctgg
      241 tgacgatcag cggaaccgga tttaccggcg cgactgccgt gacgttcaat ggaaccagcg
      301 cttcgttcac ggtggtgaac agtacgacga taacggcaac cgtaccggcc ggggccacta
      361 ccggtgtcgt tgccgttacg gcgccctgcg gaacagcctc ctcgggaaca gcctttacca
      421 ttggatcatc caccacgttg aatgttcacg tactgattga aggctaccac ctcggtggag
      481 gagtcatgcc ggaagtctcg gctaccggtg tttgtgatac ggtgaccgtc gtccttgctg
      541 cagccgccag tccgggcgtt gactttgcaa ccgcccgcgc agcgatcaat accagcggtt
      601 ggggtgcgtt cgtcttcagc ggcctcacac cgggttcgta ttatatcggt gtgcggcacc
      661 gcaacagtat tgaaacctgg agcgccagtg caatcagctt atcagcagga agcgccactt
      721 acgatttcac cacagcaagt acacaggctt acggtggcaa cctgagaaat ctgggcggtg
      781 gccggttcgc gttgttcagt ggcgatgtca accaggacga cttgattgaa tctgcagatt
      841 atggtgccgt ggaaaatgcg gttgttggct tcttatcggg atatcaggtc gaagacctta
      901 caggcgataa cctggtcgag tcgaccgact attcattgat tgaaaacaac ttgatgggat
      961 tcatcttcag tattcgtccc tgataccaag ggcctcagcg ttgctgtccg attaactggc
     1021 tggctgcggc atagccctgt ttggctttct cgttgttcgg attgatgttg gtcaacagga
     1081 attcccaggt cgatcgggct ttttcaagtt gccctcctga gaaatacgcg ccgcccaggt
     1141 tgtataagat gttctcattc cgatcatcca gctgacgcgc ccattccagc cactggatgg
     1201 ctccggcaaa gtccttgttc gatcctttca tgagcgcgtt gttcatgagc acctctgcgt
     1261 aggtcctgag cgcggggtcg ttgtggtggt attctttcgc cttcagccag gctgcaacag
     1321 cttccggata cttaccctgg taaaagtaag ccactcccat attggaatag cctttcggga
     1381 atttcggata gatgtcgacg gaacgttgta agtagcgaat gccttcttcg atgtaggctt
     1441 gttcctgcgg agtaccttta atgccttctt tctgactttc atccagcagg ctcttgccgg
     1501 cattcgcatt gaccagtacg gagttcgaag acaat
//
LOCUS       DAOTON010000750         1529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_64778_length_1529_cov_3.340570, whole genome shotgun sequence.
ACCESSION   DAOTON010000750 DAOTON010000000
VERSION     DAOTON010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64778_length_1529_cov_3.340570"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="P5292_12375"
     CDS             <1..630
                     /locus_tag="P5292_12375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS39967.1"
                     /translation="KDKDSFDEAESQSSWDKNAALYAEKFLDPALYKTGYDALLSRLP
                     KEARVLDAACGPGIMAHYFLKQRPDLRITGIDYAPAMVEQARHLVPAAQFVQGDLRSL
                     PIPAQPYHAIVCGFGLPYLSGAEVREFLRYCRANLVANGQLYLSIVVEPGGETGFRTG
                     STGDRVWFNNHEPEALRADLANEGFQLTDTWTVHPDAGNGRYEIMICIV"
     gene            786..1151
                     /locus_tag="P5292_12380"
     CDS             786..1151
                     /locus_tag="P5292_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019991177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRS39968.1"
                     /translation="MSPQPRRDIFQAIADPTRRAIISLLVLQAMTPNALAENFSVSRQ
                     AISKHVRILDECGIIESRQQGREIYYQVPPEKLKSVADWIEQFRQLWEQRYTQLDDLL
                     LQLKNKTNANDNPSSDANC"
     gene            1120..>1529
                     /locus_tag="P5292_12385"
     CDS             1120..>1529
                     /locus_tag="P5292_12385"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRS39969.1"
                     /translation="MTTPPPTRIVKDLANRKLIIHRTFLGPIDLVWRAWTEKDLLEQW
                     WAPKPFKAVTKSYDFREGGHWLYYMLGPDGSKMFACFNFHTIQPQRGFTGDDFFCDEN
                     GVRTDLYPGMAWKNEFLPVPEGTEVTVDVTFQSEA"
BASE COUNT          370 a          447 c          370 g          342 t
ORIGIN      
        1 aaggataaag actcgtttga tgaagcggaa tcccagtcca gttgggataa aaacgccgcc
       61 ttatacgcgg agaaatttct cgaccctgcg ctttacaaga cgggatacga tgcactcttg
      121 tcgcgtttac caaaagaggc acgtgtgctg gatgctgcct gcggtcccgg catcatggcc
      181 cactactttt taaaacagcg acccgatctg cgtatcaccg gcatcgacta tgcccctgcc
      241 atggttgagc aggcacgaca cctcgtgccg gctgcgcaat tcgtacaggg ggatcttcgt
      301 tccttgccga taccggcgca accctatcat gcgatcgtgt gcggcttcgg tcttccgtat
      361 ctctccggcg cggaggtgcg cgagttctta cgctactgtc gggcgaacct ggtcgccaac
      421 ggacaactct acctcagcat cgtggtcgaa cccggaggcg agaccggctt tcgtaccggt
      481 agcaccggcg accgggtgtg gttcaataac cacgaaccgg aagccctccg agcggatctg
      541 gccaacgaag gattccaact aacagacacc tggaccgtac accccgacgc gggaaatggc
      601 cgctacgaaa tcatgatttg catagtttaa cccggatcct tagaccccct agtatttttt
      661 aattcactga ttttcaatta ctaataacta attactaatt actaattact aattactaat
      721 ttttattctc cattcctttt gcaaccatat aattgcacat atatttgcaa ccatatagtt
      781 gcacaatgtc accccaacca cgccgcgata ttttccaggc cattgccgat cccacccggc
      841 gcgcgatcat cagtctgttg gtcctgcaag ccatgacgcc gaacgccttg gccgagaatt
      901 tctccgtctc gcgtcaggcg atatccaagc acgtccgcat cctcgacgaa tgcggcatca
      961 tcgagagcag gcagcagggt cgcgagatct attaccaggt accgcccgag aagctgaagt
     1021 cagtggccga ctggatcgaa caattccgtc aactctggga acaacgttac acccagctcg
     1081 acgacctcct ccttcaacta aaaaacaaaa ccaatgcaaa tgacaacccc tcctccgacg
     1141 cgaattgtta aggatctcgc gaaccgcaaa ctgatcatcc accgtacgtt cctgggtccg
     1201 atcgacctgg tctggcgtgc atggacggaa aaagacctgc tcgaacaatg gtgggcgccg
     1261 aagcccttca aggcggtcac caaatcgtac gacttccgcg aaggcggaca ctggctctat
     1321 tacatgctcg ggccggacgg ttccaaaatg ttcgcgtgct tcaatttcca cacgatccag
     1381 ccgcagcgcg gatttaccgg cgacgatttt ttctgcgatg agaacggcgt acgtacggat
     1441 ctttatcccg gaatggcttg gaagaatgaa ttccttccgg tgccggaagg aaccgaagtg
     1501 acggttgatg tgaccttcca atcggaagc
//
LOCUS       DAOTON010000751         1528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_64855_length_1528_cov_2.875764, whole genome shotgun sequence.
ACCESSION   DAOTON010000751 DAOTON010000000
VERSION     DAOTON010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64855_length_1528_cov_2.875764"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="P5292_12390"
     CDS             complement(<1..699)
                     /locus_tag="P5292_12390"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRS39970.1"
                     /translation="MRNTHLRFLAALPFVFLFLLSDQSNAQVRFDRVDNVAVEIAGTP
                     MQNPWSGGINYPLTSSVDMDGDGRKDLFVFDRFNNRVSCFLNTGSTNAVTAWQYAPEM
                     VQRFPPINKWALFYDYNCDGKEDLFMLSSQFPSGIAAWRNDYTPGSGLQFTLVNPFMR
                     ETFAGFPTNIFASAVSIPAFADVDSDGDMDIIGYNSVPDGRFIYHRNLSMDNYGTCDS
                     LVYEWASACWGNFAL"
     gene            851..1342
                     /locus_tag="P5292_12395"
     CDS             851..1342
                     /locus_tag="P5292_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HRS39971.1"
                     /translation="MPSFDIVSKIDVQKLDNAINVAIKELANRFDFHGSKTNVELDKK
                     TLQIHVLTENDMRLKAVEGIIIGRMVKQGLDPQSLDFGKELYASGNMVKKDIKVKQGV
                     DKDTAKKVVKIIKDTGLKVQASIMDEQVRVTGKKIDDLQELISILEKSQVGIPMQYEN
                     FRS"
BASE COUNT          402 a          421 c          409 g          296 t
ORIGIN      
        1 cagggcgaaa tttccccagc aggcggaagc ccattcgtac accagcgaat cgcaggtgcc
       61 atagttgtcc atggacaggt tacggtgata aatgaatcgg ccatccggaa ccgaattgta
      121 tccgatgata tccatgtcgc cgtcgctatc cacatccgca aaggccggga tggaaaccgc
      181 gctggcgaag atgtttgtcg ggaagccggc aaaagtctcc cgcatgaacg gattgacgag
      241 ggtgaactgc aaaccggagc caggcgtgta gtcgttgcgc caggcggcga tgcctgacgg
      301 aaactgggaa gagagcatga acaggtcttc cttcccgtcg cagttgtaat cgtagaacag
      361 cgcccacttg ttgatcggag ggaatcgctg caccatttcc ggcgcgtact gccaggcggt
      421 aactgcattc gtcgagcctg tattcaggaa gcaactcacc cggttgttga agcggtcgaa
      481 cacgaagagg tctttgcgtc cgtcaccgtc catatccacg gaagaggtca gcggataatt
      541 gatcccgcct gaccaggggt tctgcatcgg ggtcccggcg atctccaccg cgacattatc
      601 gacccggtcg aagcggacct gggcattgga ctgatccgag agcaggaaca ggaagacaaa
      661 cggcagcgcc gccaggaaac gtaagtgcgt atttcgcatg attacgaaga tactcaatag
      721 gcaggcggcg aaagcaggac aaatgtcaat tcttattcat tttccaacat gaacaatccg
      781 ctcccggctg gaattttagg gcagttttgg tccggctcgc gtatctttac aagaccacac
      841 acgcacgccc atgccttctt ttgacatcgt ttccaaaata gacgtccaaa aactcgacaa
      901 cgccatcaac gtggcgatca aggaactcgc caaccgtttc gattttcacg gttccaagac
      961 caacgtggaa ctcgacaaga agacgctcca gatccacgtc ctcactgaaa acgacatgcg
     1021 cctcaaagcc gtggaaggca tcatcatcgg ccgcatggtg aagcagggcc tcgacccgca
     1081 gagcctcgac ttcggcaagg aactctacgc ctccggcaac atggtgaaga aggacatcaa
     1141 ggtcaagcag ggcgtcgaca aagacaccgc gaagaaagtc gtcaagatca tcaaagacac
     1201 cggcctcaaa gtccaggcgt ccatcatgga cgaacaagtc cgcgtcaccg gcaagaagat
     1261 cgacgacctc caggaactca tcagcatttt ggaaaagtca caggtcggca ttccaatgca
     1321 gtacgagaat ttccggagtt gatcgtatgc atccaggcgg tgcatgacaa agatcaattt
     1381 tccaaccacc accccgcctc gtaggggcgg cggtatcctt gtaccggttg tccctctcgg
     1441 attttcgacc ccgtaggggt cgcggtatga aaagttggtg ctgtgataaa aaaaataata
     1501 catcatcgcc ggtgaatcgt ggcaaacc
//
LOCUS       DAOTON010000752         1525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_65020_length_1525_cov_2.738776, whole genome shotgun sequence.
ACCESSION   DAOTON010000752 DAOTON010000000
VERSION     DAOTON010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65020_length_1525_cov_2.738776"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="P5292_12400"
     CDS             complement(<1..207)
                     /locus_tag="P5292_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39972.1"
                     /translation="MVTHHQAISPLYEEKQYLGYNSLSLLRRLMLTLFCFGVYWWKKM
                     NNQNGDLFFWLGVGIIVVSILLLFV"
     gene            complement(183..>1525)
                     /gene="tilS"
                     /locus_tag="P5292_12405"
     CDS             complement(183..>1525)
                     /gene="tilS"
                     /locus_tag="P5292_12405"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRS39973.1"
                     /translation="RLIISVTEFIRRHRLFGKQDRMLVGVSGGMDSMVLADVLHRTGY
                     RIGIAHVNFRLRGHESEQDALFVSDWAKSRGIPVHPLEASAKDYARTHRLGLQEAARE
                     IRYKYFEKICRQEDYQLVAVAHHRDDAIETFFMHLMRGAGLRGLSSIPIRQAKVVRPL
                     LGTERAEIRSYAKRMKLSWREDSSNQEEYYERNRIRKNLLPKLEAHAESGLAGISRSL
                     DHLRFAREVFEHAISGMRKRYVRTLPDGTRYLSLPELRDHPFGGPLLHELLRGSGFEP
                     GEPEKVLQARRSGIRFKTQAGYLMIDRERLILHPFDVVANAERRIGPRTRKIELTGGT
                     LNISRKKWQPAAEIPNSPGEYWLDLAQLTFPLTVRPWKAGDRFRPLGMKGSRKLSDYL
                     TDRKYSLRQKEKVQVLLSEGRIVCILGERISEDVRITDSTRNILRLQWSPIIKP"
BASE COUNT          336 a          446 c          402 g          341 t
ORIGIN      
        1 cacgaacaac aacaggatcg aaaccacgat gatgcccacg cccagccaga aaaacagatc
       61 gccattctgg ttattcattt tcttccacca gtaaactccg aaacagaaaa gcgtcaacat
      121 cagcctgcgc aggagcgaaa gcgagttgta cccgaggtac tgcttctctt cgtagagcgg
      181 acttatggct tgatgatggg tgaccattgt aatcggagga tgttccgggt ggaatccgtg
      241 atgcggacgt cctctgaaat ccgctctcct aagatacaca cgatccggcc ttccgacaac
      301 aaaacctgca ccttttcttt ctggcggagg gaatacttgc ggtcggtcag gtaatcactc
      361 agcttccggc tgcctttcat cccgagcgga cgaaaccggt caccggcttt ccagggacgt
      421 acggtcaggg gaaaggtgag ttgagcaagg tccagccaat attcccccgg ggaattgggg
      481 atttcggctg ccggctgcca tttttttctg gaaatattca aggtgccgcc cgtcagctcg
      541 atcttccgcg tgcgtggtcc aatcctccgt tcagcgttcg ccacaacgtc gaaggggtgc
      601 aggatcagcc gctcccggtc aatcatcaga tagccagcct gtgtcttgaa ccggatcccg
      661 gatcggcggg cctgcagcac tttttcaggt tcacccggct caaaaccact tccgcgcagc
      721 agttcatgga gtaggggacc gccgaacgga tgatcgcgca attccggtaa cgaaaggtat
      781 cgggtgccat ccggtaaggt tcgaacatac cgcttccgca taccggaaat cgcatgttca
      841 aaaacctccc gggcaaaccg caggtgatcc aggctacggc tgatgcctgc gagacccgat
      901 tcagcatgtg cctcgagttt aggcaacagg tttttacgga tccggttgcg ttcgtagtac
      961 tcttcctggt tgctcgaatc ctcccgccag ctcagcttca ttcgtttggc ataggaacgg
     1021 atctccgccc gttcagttcc taacaagggg cggacaacct tggcttgcct gattggaata
     1081 ctgctcaacc ctcgcagtcc tgcgccacgc atcaggtgca tgaagaatgt ttcgatcgca
     1141 tcatcgcgat gatgggcgac cgcgaccagt tgataatctt cctgtcggca gattttttcg
     1201 aagtacttgt agcggatctc gcgcgccgct tcctgtaaac ccaggcgatg ggttcgggcg
     1261 tagtctttgg cggatgcttc cagtggatga accggaatcc cacggctttt cgcccagtcc
     1321 gaaacgaaca gcgcgtcttg ctccgattcg tgaccgcgta atctgaagtt cacatgcgca
     1381 atgccgatcc ggtagccggt gcgatgcagc acatcggcca gtaccatgga atccatcccg
     1441 ccgcttacgc ccaccagcat ccgatcctgc ttcccgaaca ggcgatgccg acggataaat
     1501 tccgtaaccg aaataatcag ccggt
//
LOCUS       DAOTON010000753         1524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_65080_length_1524_cov_3.789653, whole genome shotgun sequence.
ACCESSION   DAOTON010000753 DAOTON010000000
VERSION     DAOTON010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65080_length_1524_cov_3.789653"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..640
                     /locus_tag="P5292_12410"
     CDS             269..640
                     /locus_tag="P5292_12410"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRS39974.1"
                     /translation="MIQSFKDLQIWNRSVDLSIEIYQATATFPKSELFGLTSQIRRAG
                     VSPAANIAEGWSRKTSRSFHHFLDISRGSLAELYTHLEISRRLDFIPDLSAQRLMVES
                     DEISKMIHVMQLKIDPQHKLR"
     gene            738..1496
                     /locus_tag="P5292_12415"
     CDS             738..1496
                     /locus_tag="P5292_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRS39975.1"
                     /translation="MAETINLKLKIWRQKNTADRGTFELYDMPGVSTHMSFLEMMDVL
                     NDRLIREGKDPVAFDHDCREGICGMCSMYINGRAHGPLKGTTTCQLHMRHFSDGETVT
                     IEPWRAEAFPVIKDLVVDRTAFDKIIAAGGYISANTGNAPDANAIPIEKAKSDEAFDA
                     AACIGCGACVASCKNASGMLFLSAKVSHLALLPQGEPERRERVLKMIIAHDESGFGSC
                     TNTGACEAECPKGISISHIARMNREYVRASLTKE"
BASE COUNT          414 a          422 c          384 g          304 t
ORIGIN      
        1 gcgggagcga attttacctg ccgaagcttt agcgtaggca ggagcgaata gcgaatagcg
       61 aacagcgaat agtaaaaagt gaatagtgaa ttttacctgc cgaagctttt gcgtaggcag
      121 gaacaaatag taaaaagtga atcttgtcca ccgatgcttt tgcttaggtg ggagtggtag
      181 aaatagaatc cattattaat tctaataaaa gaaacagcat ctatggcgtt gtgaaaatgg
      241 gtgggcggtt ggaatctttg aaaaaaagat gatacaaagc ttcaaagacc tccaaatctg
      301 gaaccgatcc gttgacttat cgatcgagat ctatcaggca actgctacct tcccaaaatc
      361 tgaactcttc ggactaacca gtcagatacg cagagcaggg gtttcaccag cagcaaatat
      421 cgctgaaggc tggagccgga aaaccagcag aagctttcac cacttcctcg atatctcccg
      481 aggatcactc gccgaactct atacgcactt ggaaatatcc cgacgattgg atttcattcc
      541 tgatctctcc gcccaacgac taatggtcga gagtgacgaa atcagcaaga tgatccacgt
      601 catgcaactt aaaatagacc ctcaacataa gctgagataa taatcggatc gaaccgcagc
      661 aactgcgatc ccaaatacta ttcgctgttc gctattcgct attcgctccg ctcgaacctg
      721 actaaataaa ccaacatatg gcagaaacca tcaacctcaa actgaagatc tggcgtcaaa
      781 agaacactgc tgaccgcgga accttcgaac tttacgacat gccgggcgtg agtacgcaca
      841 tgtcgtttct cgagatgatg gacgtgttga acgaccgcct catccgggaa ggaaaagatc
      901 cggtcgcgtt cgaccacgat tgccgcgaag gcatctgcgg catgtgctcg atgtacatca
      961 acggccgtgc acacggaccg ctgaagggaa ccacgacctg ccagctccac atgcgccact
     1021 tctccgacgg agaaacggtc accatcgaac cctggcgcgc cgaggcattc ccggtcatca
     1081 aggacctggt ggtcgaccgc accgccttcg ataagatcat cgccgccggc ggttatatct
     1141 ccgccaatac cggcaatgct cccgacgcca acgcgattcc gatcgagaag gcgaagtcgg
     1201 acgaagcctt cgacgcggcc gcctgcatcg gctgcggcgc ctgcgtggcc tcctgcaaga
     1261 acgcttccgg catgctgttc ctctccgcaa aagtctccca cctcgcgctg ctgccgcaag
     1321 gcgaacccga gcgccgcgaa cgcgtgctga agatgatcat cgcccacgac gaatccggct
     1381 tcggcagctg caccaacacc ggcgcctgcg aagcggaatg tccgaaaggc atttccatca
     1441 gccacatcgc ccggatgaac cgggagtatg tgcgggcgtc gttgacgaag gagtgaggtt
     1501 agtgaacagt gaacagtgaa cagt
//
LOCUS       DAOTON010000754         1524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_65096_length_1524_cov_2.556841, whole genome shotgun sequence.
ACCESSION   DAOTON010000754 DAOTON010000000
VERSION     DAOTON010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65096_length_1524_cov_2.556841"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /gene="nosD"
                     /locus_tag="P5292_12420"
     CDS             <1..882
                     /gene="nosD"
                     /locus_tag="P5292_12420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HRS39976.1"
                     /translation="TLENNTIENGFFGIYLANASYCTVRNNRIYGQARTESGSGNGIH
                     VWKSHHVFIDNNIIDRHRDGIYFEFVTDSKVYRNLSTHHLRYGIHFMFSDHDGYFYNT
                     FRQNGSGVAVMYSHHVDMRHNDFSDNWGGAAYGLLLKEISHSSLIDNRFYGNTTGLYL
                     EGSSDLVAERNFFSRNGWALRILGDCFEDTVRANTFQSNSFDVATNAERNMNYCHGNY
                     WDKYRGYDLDKNGIGDVPYHPVSLFSRIAEQAPYALMLLHSFLLDIFEQAEKIVPTIT
                     PAAFNDANPVLHPTVHD"
     gene            875..>1524
                     /locus_tag="P5292_12425"
     CDS             875..>1524
                     /locus_tag="P5292_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRS39977.1"
                     /translation="MIEIRELKKSFGSLQVLRSLNLELQAGQIVCLIGPNGSGKTTLI
                     KCLLGHVLPDSGTIRVAGEQLVAGSWSYRERIGYMPQIGRYPEQMRIRQVFSMMRDIR
                     NVNTSSDEELYQRFGLEALQEKRMGNLSGGTRQKVSAALAFLFRPDLVVLDEPTAGLD
                     PVSSELLKDKLRAERERGTLTLITSHNMSEVEELADRVVFLVDGNLLNPRFLPNCSV"
BASE COUNT          347 a          390 c          406 g          381 t
ORIGIN      
        1 actcttgaaa acaatacgat cgagaacggt ttcttcggga tttatctggc caacgcgtca
       61 tactgcacgg ttcggaataa ccggatctat ggtcaggcgc gtacggaatc cggttccggc
      121 aacggtatcc atgtttggaa atctcaccat gttttcattg acaataatat cattgatcgc
      181 catcgggacg ggatctattt tgaatttgtg acggacagca aagtgtaccg caacctgagt
      241 acccatcacc tgcggtatgg aatccacttc atgttttccg accacgatgg ttacttctac
      301 aatactttcc ggcaaaatgg ttcaggtgtc gctgtcatgt attcccacca tgtcgacatg
      361 cgccataatg atttctcgga caattggggc ggtgccgcgt acggcttatt gctaaaggag
      421 atctcccaca gcagcctgat cgacaaccgg ttttatggca ataccaccgg gctttacctg
      481 gagggctcca gtgacctggt cgccgagcgg aatttcttca gcaggaacgg ttgggcattg
      541 cgtatactgg gtgattgctt cgaagatacg gtccgcgcca acacctttca atcgaactca
      601 ttcgatgtcg ccacgaatgc agaacgtaac atgaactact gccatggtaa ttactgggac
      661 aagtaccgcg gttatgatct cgacaagaac gggataggag atgtaccgta tcatccggtt
      721 agcctgtttt cccggatcgc cgaacaggca ccctacgcgc tcatgttgtt gcatagtttc
      781 ttgctggata ttttcgagca ggcagagaag atagttccga ccattacgcc ggctgcgttc
      841 aacgacgcca acccggtctt gcaccccact gtccatgatt gaaatccgag agctgaaaaa
      901 gtcgttcggt tccctgcagg tactgcggtc cctgaacctt gaactccagg ccggtcagat
      961 cgtttgcctg atcggtccta acggctcggg gaagacgacc ttgatcaaat gtctgctcgg
     1021 gcatgtatta ccggacagtg gtacgatccg cgtggcgggt gaacagcttg ttgcgggatc
     1081 ctggagttac cgggaacgca tcggctacat gccccagatc gggcggtatc cggagcagat
     1141 gcggatccgc caggtgttca gcatgatgcg ggatatccgg aatgttaaca cttcatccga
     1201 cgaggagttg tatcagcgat tcggtctgga agcattgcag gagaaacgca tgggtaacct
     1261 ctcgggtggt acccgccaaa aggtcagtgc ggccctggct tttctctttc gaccagacct
     1321 ggtcgtgctc gatgagccta ccgcaggttt ggatccggtt tcatccgagc tgcttaaaga
     1381 caaactcaga gcggagcggg agcgcgggac gttgaccttg atcacctccc ataacatgag
     1441 tgaagtggaa gaactcgccg accgtgtcgt tttccttgtt gatggcaact tgttaaatcc
     1501 tcgtttttta cctaattgta gcgt
//
LOCUS       DAOTON010000755         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_65211_length_1522_cov_2.833674, whole genome shotgun sequence.
ACCESSION   DAOTON010000755 DAOTON010000000
VERSION     DAOTON010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65211_length_1522_cov_2.833674"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="P5292_12430"
     CDS             complement(<1..708)
                     /locus_tag="P5292_12430"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HRS39978.1"
                     /translation="MRFIYCFLYFLAISPFLSYGQGGGGEVFLRPGPDCGKDALVWNA
                     PGFNNANINYGDDPSIGAHAWTNTGIPDTGRSLIEFELDQIPVGALITSAYLSLYNDS
                     SLAYCYGSHASLSGPNTGRIRRVISPWNEHLVKWFTQPSTTALHEVIVPATAGPHSNY
                     QSIDVTALVQDMVNDPDSSFGFMLSLEDETYYRALTFASSDDVNPSMRPSLLIIYQIP
                     ASGCFRLQAMNECGIDAL"
     gene            complement(762..>1522)
                     /locus_tag="P5292_12435"
     CDS             complement(762..>1522)
                     /locus_tag="P5292_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRS39979.1"
                     /translation="ILMITGPNMSGKSALLRQAALITLLAQIGSFVPATGASVGIVDK
                     IFTRVGASDNISSGESTFMVEMNETASILHNLSDRSLLLLDEIGRGTATYDGISIAWA
                     IAEFLHEHPSVRAKTLFATHYHELNEMAATLPRIRNFHVAVKETGQQVLFLRKLVPGG
                     SEHSFGIHVAKLAGIPVKVVQRAGEILKQLEDSRGRGGAKVGKDENVQLSFFQLDDPV
                     LEQIREEIRSTDLDHLTPVQALNKLNEIKKLIGG"
BASE COUNT          336 a          424 c          436 g          326 t
ORIGIN      
        1 aagggcgtca atgccgcact cgttcatagc ctgcaggcga aagcaacccg atgccgggat
       61 ctgataaatg atcaagaggg acggccgcat ggaaggattg acatcatcac tgctggcaaa
      121 ggtgagggcg cgatagtagg tttcatcttc cagactcagc atgaaaccga aagaagaatc
      181 cggatcgttc accatatcct gtaccaaagc ggtgacatcg atactttggt aattggaatg
      241 tggtcctgcg gtcgcgggta caatcacttc gtggagagcg gtcgtgctcg gctgtgtaaa
      301 ccattttacc aggtgttcat tccagggcga gatcacacgc cgtatccgac ccgtgttagg
      361 acccgataag gatgcatgac ttccgtaaca ataagcaaga gaagaatcgt tgtagaggga
      421 aaggtaagcc gaggtgatca gggcacctac cggtatctga tccagttcga actcgatcaa
      481 agaccggccc gtgtccggaa tcccggtatt ggtccaggcg tgcgccccga tggagggatc
      541 gtcgccgtag ttgatgttcg cgttattgaa accgggcgca ttccagacca gggcatcctt
      601 gccgcaatca ggtccgggcc ggaggaacac ttcgcctcca ccgccctgcc cgtaggacag
      661 aaaaggtgat atagccagga agtataggaa acagtagatg aaacgcatgt ggtgaactat
      721 actggaaagg tactgttttc cagccaactg cgtactgtgg ctcacccccc gatcagcttc
      781 ttgatctcat tcagcttgtt cagcgcctgt acgggagtca gatggtcgag gtcggtggaa
      841 cggatctctt cacgaatctg ctccaacacc ggatcgtcga gctggaaaaa gctgagctgc
      901 acgttctcgt cctttccgac tttcgctccg cccctgcccc ggctgtcttc cagttgtttc
      961 aggatctcgc cggcacgttg aaccactttc accggaatac cggcgagctt ggcgacgtga
     1021 atgccgaagc tgtgctcgct gccgcccggc acgagcttcc gcagaaacaa tacctgttga
     1081 ccggtttcct tcacggctac atggaaattc cggatgcgcg gcaacgtggc cgccatctcg
     1141 ttcaactcgt gatagtgcgt ggcgaagagg gtcttggccc gtacgctcgg atgttcgtgc
     1201 aggaattccg cgatcgccca ggcgatgctg ataccgtcgt acgttgccgt gccgcgaccg
     1261 atctcgtcga gcaacaacag actacggtcg gataggttgt gcaggatgct cgccgtttcg
     1321 ttcatctcca ccatgaaggt ggattcgccg ctcgagatgt tatcgctcgc gcctacgcgc
     1381 gtgaagatct tatcgacgat gcccacgctg gcacccgtgg cgggcacaaa cgaaccgatc
     1441 tgcgccagca gggtgatcaa ggcggcctgc cgcagcagcg ccgacttacc cgacatgttc
     1501 gggccggtga tcatcaggat ct
//
LOCUS       DAOTON010000756         1518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_65471_length_1518_cov_3.580314, whole genome shotgun sequence.
ACCESSION   DAOTON010000756 DAOTON010000000
VERSION     DAOTON010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65471_length_1518_cov_3.580314"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..281)
                     /locus_tag="P5292_12440"
     CDS             complement(84..281)
                     /locus_tag="P5292_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39980.1"
                     /translation="MKAKSINNPEFFHRMAELVAIANAGVRNALEENRRRRIPIVFSL
                     LGKIYYRLPDGTITHKSPFKG"
     gene            complement(278..859)
                     /locus_tag="P5292_12445"
     CDS             complement(278..859)
                     /locus_tag="P5292_12445"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS39981.1"
                     /translation="MSPKKELVIIAGPNGSGKTTFARAFIREKGYRFLNADEINKELQ
                     IENKNSNSIAAGKEYFKRLDLLLDKNKNLIIESTLSGKFMGRLIKRLEQKNYNITIIF
                     VYLDSPQLCIARIKERVKKGGHAVPDSDVTRRYFRSCSNFWNQYKNKAAQWLLLNNSG
                     DQIKRIAEGSGRSMLVNDEALHVLFLKNTLSKK"
     gene            complement(872..>1518)
                     /locus_tag="P5292_12450"
     CDS             complement(872..>1518)
                     /locus_tag="P5292_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRS39982.1"
                     /translation="VYGIMIGGWASNNKYSLMGALRASSQMISYEIAMGLAIIALVMM
                     TGTLSVREIAEQQAGAHWNVFYQPLGFLIFIVCAFAECNRTPFDLPECESELVGGYHT
                     EYSSMKLGFYLFAEYINMFISSAVISTLYFGGYNMPFIGRFVTDPNILSILGTVFLFG
                     KIFFFIFFFMWIRWTIPRFRYDQLMNLGWKNLIPLAIANIFITGLVMWLTGKVS"
BASE COUNT          367 a          329 c          406 g          416 t
ORIGIN      
        1 atggccggca ggtagatctt ttccgaaaag gtcatttcct ttttggcgac gactttgctt
       61 cggttggtca gtgattgcat cgcttatcct ttaaaaggac ttttatgggt aatggtgccg
      121 tccggcaagc ggtaataaat tttgccaagg agggagaata ctattggaat tctccggcga
      181 cggttctctt caagagcatt tctgacgcct gcattcgcga tagctactaa ttcagccatc
      241 cgatgaaaga attctggatt gttgatagac tttgctttca ttttttagaa agtgtgtttt
      301 taaggaagag gacgtgtaat gcctcgtcgt taacaagcat tgatcttccg gatccctccg
      361 caattcgttt gatctggtct ccagagttat ttaacagcag ccattgggca gccttatttt
      421 tatattgatt ccagaagttg gaacagcttc tgaaataacg tcgggtgacg tcggaatctg
      481 gtactgcatg cccgcccttt tttacgcgtt ctttaatcct ggctatgcac aattgaggag
      541 agtccagata aacgaagata atggtaatgt tatagttctt ttgttcgagc cgtttgatca
      601 ggcgtcccat gaatttgcct gaaagggttg actctatgat gaggttttta ttcttatcga
      661 ggagtagatc aagtcgtttg aagtattcct tgccggcagc aatggagtta ctgtttttat
      721 tttcaatttg aagctctttg tttatttcgt cagcattcag gaagcggtat cccttctctc
      781 ggataaaagc tcgtgcaaag gtggtttttc cgctgccatt gggtcctgca ataatgacca
      841 gttctttctt cggactcacg gattggtcga attaagagac tttcccggtc agccacatga
      901 ccagtccggt aatgaagatg ttggcaatcg ccaacgggat gaggttcttc cagcccaggt
      961 tcatcaactg gtcgtagcgg aagcggggga tggtccaccg gatccacatg aagaagaaga
     1021 tgaagaagaa gatcttgccg aagaggaaga ccgttcccag gatgctcagg atgttcgggt
     1081 cggtaacaaa tcgcccgatg aacggcatgt tataaccacc gaagtaaagg gtcgagatca
     1141 ccgcggagga gatgaacatg ttgatgtatt cggcgaagag gtagaagccg agtttcatcg
     1201 agctgtattc ggtgtgatag ccgcccacca gttcgctttc gcattcaggc aggtcgaagg
     1261 gcgtacggtt gcattccgcg aaggcgcaaa cgatgaagat caggaagccc agcggctggt
     1321 agaagacatt ccagtgagcg ccggcctgtt gctcggcgat ctcgcgaacg ctaagggtgc
     1381 cagtcatcat gacgagggcg atgatcgcaa gacccatcgc gatctcgtag gagatcatct
     1441 gggacgaggc gcgcaaggcg cccatcaggg agtatttgtt gttcgatgcc cagccgccga
     1501 tcatgatgcc ataaacac
//
LOCUS       DAOTON010000757         1517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_65560_length_1517_cov_2.506156, whole genome shotgun sequence.
ACCESSION   DAOTON010000757 DAOTON010000000
VERSION     DAOTON010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65560_length_1517_cov_2.506156"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1517
                     /locus_tag="P5292_12455"
     CDS             <1..>1517
                     /locus_tag="P5292_12455"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39983.1"
                     /translation="GASNSNAGNADILIFKLNQDLSSVQWMKNYGGSSADVATIMLLN
                     NANGDLFLGGYTNSTNFPVSNARQSTLAGSQSGILQKLDGNGNVAWSSYFQSASNRST
                     SILCMEFSLLKDQLYFGGITTGLAASNISSFGVLDNTQNGSNDFFVCRMDTNQNFVAS
                     TYLGGTGSEGNMMGLNTDQNNDVYIFGYSGSSDFPVSGLPNTPLQTTNLGSNDKTFTK
                     INSDLSSLVFSTYYGGSNDDYDPVGERGIKFSNCRIYTIVTSGSNNIPLTTGALNTTK
                     NSNVGLYEPGLVVWANPPDLLGNSITGTQGICAGNTPGDLSGSEPAYVLPEISRNGTG
                     SSYPSLGSAATYQWQISTDSVTWNDIPGATSQNLLGSQIGPLFEKTFFRRIIGGDACI
                     LAGVADQVVTVNIATAAGTVTNLRCFQDASGSITATSNGVAPFDYLWSDGQTTETATG
                     LSAGNYSVTVTDLNGCSATASFTLTEPDLLTVSATGSTVTCTSGPGEATASALGGT"
BASE COUNT          360 a          453 c          383 g          321 t
ORIGIN      
        1 ggtgcttcga acagcaatgc cggcaacgcg gatatcctga tcttcaaact caatcaggac
       61 ctttcatcgg tacagtggat gaaaaactac ggtggaagct ccgcggatgt tgccactatc
      121 atgttgctga acaacgccaa cggcgatctt tttctcggcg gttataccaa ctctaccaac
      181 ttcccggtca gcaacgcgcg ccagtcaaca ctcgctggca gtcaaagcgg tattctccag
      241 aaacttgacg ggaacggtaa cgtagcctgg tcgtcttact tccaatcggc cagcaaccgc
      301 agcacctcga tcctctgcat ggagttcagc ctgctgaaag accagttata tttcggcggt
      361 atcacaacag gcctcgccgc ttcgaatatc tcctctttcg gagtactcga caatacgcag
      421 aacggcagca acgacttctt cgtctgccgc atggatacga accagaattt tgtcgcatcg
      481 acgtacctcg gcggcaccgg tagtgaggga aacatgatgg gtctgaacac agaccagaat
      541 aacgacgtgt acatcttcgg ttactccggc agcagcgact ttccggtcag cggcctgccg
      601 aacacgcctt tgcagactac taacctggga agcaacgaca agacatttac caagatcaac
      661 tccgatcttt catcattggt gttcagcacc tactacggcg gcagcaacga cgactatgat
      721 ccggtaggag aacgcggcat caaattctcc aactgccgta tctacacgat cgtaacctcg
      781 ggtagcaaca acatcccgct gaccaccggc gcactgaaca ccacgaagaa cagtaacgtg
      841 ggcctctacg aacccgggct ggtcgtctgg gccaaccctc cggatctgct gggtaattcg
      901 atcaccggca cgcagggtat ttgtgcaggt aacacgccgg gcgacctgag cggctctgaa
      961 ccggcctatg tacttccaga aatctcccgt aacggaaccg gctctagcta tccttcactt
     1021 ggctctgcag ccacctacca atggcagatc agcaccgaca gcgttacctg gaatgacatt
     1081 ccgggagcta cttcccaaaa ccttctggga agccagatcg ggccgctctt tgaaaagact
     1141 ttctttcgcc ggattatcgg tggtgacgca tgcatccttg ccggggttgc cgaccaggtt
     1201 gttaccgtaa acatcgcaac cgccgccgga actgtaacca acctccgttg tttccaggat
     1261 gcgagtggca gcatcaccgc tacttcgaac ggtgtggctc cctttgatta tctctggagt
     1321 gatggacaga cgactgaaac cgcgacgggc ttgtcggctg gaaattacag tgtcacggta
     1381 acggacctta atggctgcag cgcgactgcc agcttcacgc tgaccgaacc tgatctgctc
     1441 acggtgagcg ccaccggttc gactgttacc tgtaccagcg gcccgggtga agcaaccgca
     1501 tctgcactgg gaggaac
//
LOCUS       DAOTON010000758         1514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_65739_length_1514_cov_2.838245, whole genome shotgun sequence.
ACCESSION   DAOTON010000758 DAOTON010000000
VERSION     DAOTON010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65739_length_1514_cov_2.838245"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..>1514)
                     /locus_tag="P5292_12460"
     CDS             complement(128..>1514)
                     /locus_tag="P5292_12460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS39984.1"
                     /translation="MTGGIEPGSTGLVVNDGGVIQAGDWIETRQQNGSWDTQPISWAD
                     YSIGQILQVTSRSGDTLICDRPLRIGYDPGLNPEVSVFTPAREVGLECFSIVRSDNVT
                     AAFCPLINFRHAVNCYVKGIDVGTSISAHILLDAAAQITVTGSYFHDAYEYDGASMHG
                     YGIALYFHTSDCLIEDNIFRNLRHSLSFQCGANGNAVGYNYSRDPNRSEFPANYGADI
                     SMHGHYSYANLFEGNIVQNIQLDQTWGPSGPRNTFFRNRAELYGILMTSATMQSDSQA
                     FVGNEVPNTGLFLGNYTLAGSGHFEHGNMVRGTLTPAGTGNLPDSSYYLSGQPDWWSG
                     SSWPGIGVPFVTGSGSNPAFDRYAAGGSLTVCERSITTGLSTPGPSDYLLYPNPASRE
                     LFLRKENHEASTSGLIQLFNSHGQLVFENPLTTDQVMRIALPYLPAGSYLVKILEKGS
                     EIRQRLMLLPD"
BASE COUNT          357 a          385 c          428 g          344 t
ORIGIN      
        1 ctggctggac ctcaagcaac accacatgcg gcaccactac ctggatgcac acaatggttt
       61 cggtgttagt tcgaaattct gggacctcat cttccgtacc accttccgcc gtaaaagcga
      121 aagcacatca atcgggcagc aacattaacc gctgacgaat ctccgagccc ttttcaagaa
      181 ttttgactag gtagcttcct gccggtaaat acggaagggc aattcgcatg acttgatccg
      241 tcgtcaatgg attttcgaaa accaattgac catgcgaatt gaagagttga ataagtccgg
      301 aagttgaagc ttcatgattt tctttccgga gaaaaagttc gcgacttgcc gggttgggat
      361 aaagtaagta gtcggaagga ccgggggttg agagaccggt tgttatagaa cgttcacaaa
      421 ccgtcagcga tccaccggcg gcgtaacggt caaaggccgg gttgcttcca ctgccggtga
      481 caaatgggac accgatcccg ggccacgaac tcccgctcca ccaatccggt tgtccgctga
      541 ggtaataaga tgaatccggc agattaccgg ttccggcagg tgtcagggtt ccccgtacca
      601 tattgccgtg ttcaaaatga ccactacccg ccagggtata attgccgagg aataatccgg
      661 tattcggcac ttcattccct acgaatgcct gggagtcgga ttgcatggtg gcggaggtca
      721 tcaggatccc gtaaagctcc gcacggtttc ggaaaaacgt gttgcgtgga ccggatgggc
      781 cccaggtctg gtcgagctgg atgttttgga cgatgttgcc ttcgaacaaa ttggcatagg
      841 aataatgacc gtgcatggag atgtccgccc cgtagttggc cgggaattcg gaacggttcg
      901 gatcgcggga gtaattgtaa cctactgcat taccgttggc gccgcactgg aaggagagcg
      961 agtgccgaag attacgaaag atattgtctt caatcaggca gtcgctggtg tgaaagtaga
     1021 gcgcgatgcc atatccgtgc atggaagctc cgtcgtattc gtaggcgtcg tggaaatagc
     1081 tgccggtaac ggtgatctgc gcagcggcat caagcaggat gtgcgcgctg atgcttgtgc
     1141 cgacatcgat ccctttcacg tagcaattca ccgcgtgtcg aaagttgatc agcggacaga
     1201 aagcggccgt tacgttatcg ctgcggacga tggagaaaca ctcgagtcct acctcacggg
     1261 caggtgtaaa gacggaaacc tccggattga gccctggatc gtagccgatg cgcaacggac
     1321 gatcgcagat cagcgtgtct ccactacggc ttgtaacctg aaggatctga ccgatggaat
     1381 aatctgccca ggagatcggt tgtgtatccc aggatccgtt ttgttgtcgg gtttcaatcc
     1441 agtctccggc ctggatcacg ccgccatcgt ttacgaccag acctgtgctg cctggttcga
     1501 taccgcccgt catg
//
LOCUS       DAOTON010000759         1512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_65868_length_1512_cov_3.003432, whole genome shotgun sequence.
ACCESSION   DAOTON010000759 DAOTON010000000
VERSION     DAOTON010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65868_length_1512_cov_3.003432"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /gene="sucC"
                     /locus_tag="P5292_12465"
     CDS             complement(<1..209)
                     /gene="sucC"
                     /locus_tag="P5292_12465"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009642110.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HRS39985.1"
                     /translation="MNIHEYQGKEILKTFGVGIQEGIVADTADAAVAAAKKLSEQTGT
                     QWWVVKAQIHAGGRGKGGGVKLAKSL"
     gene            complement(301..825)
                     /locus_tag="P5292_12470"
     CDS             complement(301..825)
                     /locus_tag="P5292_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRS39986.1"
                     /translation="MIDKVKPPVKADLSEYYQSYWDYLQSDDLLLEMKNQFRESLEAF
                     GAFRATDGGPAYAPGKWSVKQLLGHLIDCERIFQFRALSCARGERAALPAFDEDLYAQ
                     TSRSDDRSLDEMLEEWSVVRRAYECLIEPFDEAEWDRAGVANGREVSNRILLYFVIVH
                     ARHHTRILHERYGK"
     gene            complement(877..>1512)
                     /locus_tag="P5292_12475"
     CDS             complement(877..>1512)
                     /locus_tag="P5292_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39987.1"
                     /translation="LYGQDCEGFYPMNKGNVMELTSYNEKDKLQSVSTTTITDRASGP
                     DGITLQMHSSSRDEKGKSMAEVDFDARCSEGVFYMSMKNMVSSEQLAAWKDMDMKMSG
                     TDLQIPLNAAPGQTLPDGSFMIEVSMNGMKMPGMSIEVTDRKVVAEETITTAAGTFTC
                     LKITSKVKSKTIFTMEMNSVEWYSKGNGMVRSESYKGDKLKGYTVLTKLVK"
BASE COUNT          320 a          423 c          374 g          395 t
ORIGIN      
        1 agggatttgg ccagcttaac gccgccacct tttccacgac caccggcatg gatctgggct
       61 ttcaccaccc accattgggt accggtttgt tcgctgagtt ttttcgcggc ggctacggct
      121 gcatcggccg tatcggccac gatgccttcc tggattccga cgccaaacgt cttgaggatc
      181 tctttaccct ggtattcgtg aatgttcatg ctgttgctgg ctgttccggc aatggtctgt
      241 tccaccccgg aagccgcgca aaagtagcag attcggggaa attacgaccg aaaaaatcga
      301 tcatttcccg tatcgttcgt gcaggatccg ggtgtggtgc cgggcatgga cgatcacgaa
      361 atagagtaaa atgcggttgg agacttccct gccgtttgcg acccctgccc gatcccattc
      421 tgcttcatca aagggctcta tcaggcattc gtaggctctc cgaacgaccg accattcttc
      481 cagcatttca tccaggctgc gatcatccga gcgactggtc tgagcataga gatcctcgtc
      541 gaaggccggt aatgccgcgc gctcccctct tgcacaagac agcgccctga attggaaaat
      601 ccgttcgcaa tcgatcaggt gacccaacag ttgtttgacc gaccatttac cgggagcata
      661 ggcagggcca ccgtcggtcg cccggaatgc tccgaacgct tccaggcttt cccgaaactg
      721 attcttcatt tccaatagca ggtcatccga ctggagatag tcccaataac tctggtagta
      781 ttcggacagg tccgccttga ccgggggctt tactttatct atcataaaga tttgtaaaat
      841 aataagttaa gagtctggtg caaataaaaa atgaagtcac ttaaccagct ttgtcagcac
      901 ggtatatccc ttcagtttgt cgcctttata agactccgac cgcaccatcc cattcccttt
      961 ggaataccac tcaacgctat tcatttccat ggtgaagatc gttttggact tcaccttcga
     1021 ggtgatcttc aggcaggtga acgtgccggc agcggtggtt atggtttcct cggcgaccac
     1081 cttgcggtcg gtcacttcga tcgacatacc cggcatcttc atcccgttca tgcttacctc
     1141 gatcatgaag gaaccatccg gtaatgtctg accgggagcc gcattgagtg ggatctgcaa
     1201 atcggttccg ctcattttca tgtccatgtc cttccaggca gccagttgtt cggacgaaac
     1261 catgtttttc atgctcatgt agaaaacgcc ctccgagcaa cgtgcatcaa aatccacttc
     1321 agccatgctt ttacctttct cgtctcgtga actgctgtgc atctgcaggg tgattccgtc
     1381 gggaccggaa gcgcggtcgg tgatggtggt ggtggagacc gactgcagct tgtccttttc
     1441 gttgtaggag gtcagctcca tcacattgcc cttattcatc gggtagaagc cttcgcagtc
     1501 ctgtccgtag ag
//
LOCUS       DAOTON010000760         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_65924_length_1511_cov_3.968407, whole genome shotgun sequence.
ACCESSION   DAOTON010000760 DAOTON010000000
VERSION     DAOTON010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65924_length_1511_cov_3.968407"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="P5292_12480"
     CDS             complement(<1..1480)
                     /locus_tag="P5292_12480"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRS39988.1"
                     /translation="MDKTVLLKLSSPDLTYGAVGIHGLQTVMSSDVANLRLDVSADSL
                     FLGADRRVVPLVDLHASAHDNRIEYLLNVSQQDSFPNRMMLAGDLDFTSSRKFSLEID
                     SSSLFLEGRHWVLNEGNQVAFDSSAIVFHALDVSNGLEALRIEGAIVKDSLHQLVVRM
                     ENFNLHHLDPVLGGTALGGRVNGDVIGVDLLRHPQLETDLTIADLTVNGDSLGNATVV
                     TRLNSEQKVVVASIGIQRGTAKVADIRGNYYLARESDNLDFRIKLENFYLRTIERYVD
                     DVVSDVNGKLSAELNLHGTLKRPVFEGELDFKRAGCVVNYLNTRYNFTSKVKVEENAF
                     NLDHMTLVDVNNNEAAVKGKILHDYFRNFRFDIQLRPRNFQALNTDPSQNELYYGRAN
                     ATGLVKITGPLEYLNMDITLSPTKGTILNIPLTSSTEVTQSNFITFIDPKKRDDFSEN
                     SNTIDLSGIRLNMNLEMNPDAEINIIFDQKIGDVISGNGNGNL"
BASE COUNT          349 a          389 c          384 g          389 t
ORIGIN      
        1 gaagattccc attaccgttt ccggagatca cgtcaccgat cttttggtcg aagatgatgt
       61 ttatctccgc atccggattc atctcgagat tcatgttcaa ccggatcccc gacaaatcga
      121 tggtgtttga attttcggag aagtcgtccc gtttctttgg gtcgatgaag gtgataaagt
      181 tgctttgcgt tacctccgtg ctgctcgtca gcgggatgtt gaggatggtt cctttcgttg
      241 gggaaagggt gatgtccatg ttcaggtatt cgagcgggcc ggtgatcttg accaggccag
      301 tggcgttggc gcgcccatag taaagttcat tctgactcgg atccgtgttc agcgcctgga
      361 aattccttgg ccgtaactgg atatcaaatc tgaaattcct gaaatagtca tgcagtatct
      421 tgcccttgac agcggcttcg ttgttattca catctaccag ggtcatatgg tcgaggttga
      481 acgcgttctc ttcgactttc accttgctgg taaaattata tcgggtattg agataattca
      541 cgacacatcc tgctcgcttg aaatccagtt ctccctcgaa gacaggcctt ttgagcgttc
      601 cgtgaaggtt cagctccgcg gacaattttc cgttcacatc cgaaaccaca tcatccacat
      661 aacgttcgat cgttcgcaag tagaaatttt ccagtttgat ccggaaatcc agattgtcgg
      721 attcccgcgc gagataatag ttgccgcgga tatccgctac tttggcagta ccccgctgga
      781 taccgatgct ggccacgacg accttctgtt cactattcaa acgggtgacg actgtcgcat
      841 ttcccaatga atctccgttg actgtcagat ccgcgattgt gaggtcggtt tccaattgcg
      901 gatggcgcag aaggtcgaca ccgatcacat cgccattcac ccgaccaccc aaagccgttc
      961 cacccaatac cggatccagg tgatggaggt tgaaattctc catacgcacg acaagctggt
     1021 gcagggaatc cttcacgata gccccttcta ttcgcagcgc ttcaagtcca ttggatacgt
     1081 ccaaggcgtg gaagacaatg gcggaagaat caaaggccac ctgatttcct tcattcaaca
     1141 cccagtgccg gccttccagg aagagcgatg aggagtcgat ctccagacta aactttcggg
     1201 aggaggtgaa atccagatcc ccggcaagca tcatccggtt gggaaaactg tcttgttggg
     1261 atacgttgag gaggtactcg atcctgttat catgggccga ggcatggaga tcaactaagg
     1321 gaacgacccg tcggtcggct ccgagaaaga gcgagtcggc agaaacatcc agccgcaggt
     1381 tggcgacatc cgagctcatg acggtttgca atccatgaat gccgacagcc ccataggtaa
     1441 gatccggcga gctcagcttg agcagtaccg ttttatccat ggtattcatt ttcccgctca
     1501 agcgtgtatt g
//
LOCUS       DAOTON010000761         1510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_65981_length_1510_cov_4.325086, whole genome shotgun sequence.
ACCESSION   DAOTON010000761 DAOTON010000000
VERSION     DAOTON010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65981_length_1510_cov_4.325086"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="P5292_12485"
     CDS             <1..629
                     /locus_tag="P5292_12485"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRS39989.1"
                     /translation="ELLLNILPEETAEELKATGTAKARNFDSVTVMFTDFKNFTQASE
                     RLSPEELVREINDCYSEFDRIVSRYGIEKIKTIGDAYMCAGGLPVPNGTHPVDVIKAG
                     LEFQAFIEQNKQKRISQGLPYFELRLGIHTGPVVAGIVGIKKFAYDIWGDTVNTASRM
                     ESSGATGKVNISGTTYELVKDHFDCTPRGKVEAKNKGWIEMYFVEGLK"
     gene            complement(630..1247)
                     /locus_tag="P5292_12490"
     CDS             complement(630..1247)
                     /locus_tag="P5292_12490"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRS39990.1"
                     /translation="MLIDNLTYASLEAEVIGRLRRDLSPEYRYHNAEHTLDVIEAAER
                     LSRMEGLDESDRLLIRTAALLHDTGYLEGRKEHEVISQRIARDYLTRLNIDEEQIRKV
                     ERMIAATRVPQQPQDLFDRILCDADLDYLGREDYFPLASRMYAEFLHDGTVRNEAEWL
                     ELQVKFLGAHRYFTDSAKRTREAGLNRQLESLRAQYERSRSAARA"
     gene            1301..>1510
                     /locus_tag="P5292_12495"
     CDS             1301..>1510
                     /locus_tag="P5292_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRS39991.1"
                     /translation="MSLNSVPYEKTLRILQQVELFEHVPTNVIEELCKKMLISSFRAE
                     DRIINQGDEGNSMYVILSGKVHIHDG"
BASE COUNT          357 a          412 c          417 g          324 t
ORIGIN      
        1 atgaactgct gctcaacatt ctgccggaag aaaccgcgga agagctcaaa gcgaccggca
       61 ccgccaaagc acgcaacttc gattcggtga cggtcatgtt caccgacttc aagaatttca
      121 cccaagccag cgaacggctt agtccggagg aattggttcg ggagatcaac gattgctaca
      181 gcgaattcga tcgcatcgtt tcccggtacg gtatcgaaaa gatcaagacc atcggcgacg
      241 cttacatgtg cgccgggggc ttgccggttc ccaatggtac gcatccggtc gacgtgatca
      301 aggccggtct ggaattccag gcattcatcg aacagaacaa gcagaagcgg atcagccagg
      361 ggctgcccta ttttgaatta cgcctgggga tccataccgg accggttgtg gccggtatcg
      421 taggcatcaa gaaattcgcg tatgatatct ggggcgacac cgtcaatact gcttcacgaa
      481 tggaaagcag cggcgccacc ggcaaggtga acatcagcgg caccacatac gaacttgtca
      541 aggatcattt cgattgcaca ccccgcggaa aggttgaagc caagaacaaa ggctggatcg
      601 agatgtactt cgtggaaggt ctgaagtgat caggcccggg cagcggaacg ggatcgttcg
      661 tattgagcac ggagtgactc caactgtcgg ttcagtcccg cttcgcgggt tcgcttggcg
      721 gagtccgtga aataccggtg cgcacccagg aatttcactt gaagctcgag ccattcggcc
      781 tcgttccgta ccgttccgtc gtgcaggaat tccgcgtaca tccggctggc tagagggaaa
      841 taatcttccc ggccgaggta atccagatcc gcatcgcaca ggatacggtc gaacagatcc
      901 tgtggttgtt gcggcacccg ggttgccgcg atcatccgct cgacctttcg gatctgttct
      961 tcgtcgatat tcaaccgggt caggtaatca cgtgcgattc gctggctgat gacttcatgt
     1021 tctttcctgc cttccaggta gccggtatcg tgtaacaagg ccgcggtccg gatcagcagg
     1081 cgatcggatt cgtccaaacc ttccattctg gacaggcgtt cggcagcctc aatcacatcg
     1141 agcgtatgct cggcgttgtg ataccgatat tccggagaga ggtcgcggcg caggcgacca
     1201 atcacctccg cttccaacga ggcgtaggtg aggttgtcga tcaacatcac cccaatttac
     1261 gaaaatccta tatatttacc ttcggcgacc cgataattgc atgtccctga acagcgtccc
     1321 ctacgaaaaa accctgcgca tcctgcagca ggtggagctg tttgagcacg ttcctacgaa
     1381 tgtaatcgag gaactctgta aaaagatgct gatttccagt ttccgggcgg aagaccggat
     1441 catcaaccag ggcgatgaag gaaacagcat gtacgtgatc ctctcgggca aggtgcacat
     1501 tcacgatggc
//
LOCUS       DAOTON010000762         1510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_65985_length_1510_cov_3.942268, whole genome shotgun sequence.
ACCESSION   DAOTON010000762 DAOTON010000000
VERSION     DAOTON010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65985_length_1510_cov_3.942268"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..633)
                     /locus_tag="P5292_12500"
     CDS             complement(10..633)
                     /locus_tag="P5292_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39992.1"
                     /translation="MASCTQCGQFTKFEGGLCYSCYAEKKNGEEPEPETMEIRALNSG
                     GLTEKERAYRYGMIKGRIAETLVQELFLSLGYNVFRYGMENTIPGIMELLKGVRSEVA
                     QEIRRMPDFVMQNPKTKDVHFVEVKFRAKGTFTFEDLPKNYPFENAFFIVVSKKHIKC
                     ISFQELKEGKSISPESRNYLGSRKEFDLDKDVIIEFCEFAVKFFSQV"
     gene            complement(693..>1510)
                     /locus_tag="P5292_12505"
     CDS             complement(693..>1510)
                     /locus_tag="P5292_12505"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRS39993.1"
                     /translation="FGRNIMSDASPVPLDQATLELLADMEPVLRRMGVRWFLIGAIAR
                     DLLLPETIHAPDYRRTQDVDLAVWLPDEAAFQSLMDRLEATGNFERDRLNSIRLHHRG
                     GMLADLIPFGDLATEQGEVHLTRPSVLTLDIPGLLEVEARIVSHQIADRTCYTCPPEG
                     LLLLKLLSNDDRPERTQDLTDIRLLLEAVFNIAAYEIATEHFDLFDQYEGTSNQSYGQ
                     EIAGHFLGRKLKSLLQSNDKLRTRILTILRKKDDAIWSAMARGLNDAFRSPGI"
BASE COUNT          353 a          406 c          368 g          383 t
ORIGIN      
        1 agtggagtat cagacctgac taaagaactt gacagcaaac tcacagaatt caatgatcac
       61 atccttgtca agatcgaatt cctttcgaga gccaagataa ttccggcttt ctggtgagat
      121 cgacttccct tcctttagtt cctgaaagct gatgcacttg atgtgtttct tggataccac
      181 gatgaagaag gcgttttcga agggatagtt cttcggtaaa tcctcgaagg tgaacgttcc
      241 tttggcccgg aacttcactt cgacaaaatg gacatccttg gttttcggat tctgcatcac
      301 gaaatccggc atccgacgaa tctcctgcgc cacttccgag cggacacctt tcagcaattc
      361 catgataccc ggtatggtat tctccatgcc gtaacggaag acgttatagc ccaggcttaa
      421 gaagagttcc tgaaccaggg tctcggcaat gcgccctttg atcatgccgt accggtaggc
      481 gcgctctttc tccgtcagac caccggaatt gagtgcgcgg atttccatcg tctccggctc
      541 gggctcttct ccatttttct tttcagcata acagctataa cacagtccgc cttcgaactt
      601 ggtgaactgt ccgcattggg tgcaactggc catgatatag agcttttaaa agtgtagtca
      661 aagtacgaat tatcgtttca attaaaccga cactatattc ctggagaccg gaacgcgtca
      721 ttcaatccac gagccatggc tgaccagatt gcatcatctt tttttcgtag aatcgtgagg
      781 attctcgtac gtagtttgtc attggactgc aacaggcttt tcagtttgcg accgaggaaa
      841 tgccccgcga tctcctgccc atagctttgg ttgcttgtcc cctcgtattg atcgaaaaga
      901 tcgaagtgtt ccgttgcgat ttcgtatgca gcgatattga aaaccgcttc gagtagcaaa
      961 cggatatccg tcaggtcctg cgttcgctcc ggacgatcat cgttactgag tagtttcaag
     1021 agtagcagac cctccggcgg acaagtgtag caggttcgat ctgcgatctg gtgactgaca
     1081 attcgggctt ccacttcgag caaacccgga atatccagcg tgagaacgct gggtcgtgtc
     1141 aggtgcactt cgccttgctc tgttgccagg tcgccaaacg ggatcagatc ggccagcatc
     1201 ccgccgcgat gatgcagccg aatgctattc aggcgatcgc gttcaaaatt tcccgtcgct
     1261 tcgagccgat ccatcaagga ttgaaaagcc gcttcatccg gcagccaaac agcaaggtcg
     1321 acatcctgtg ttcggcggta atccggtgca tgaattgttt ccggcagcag caggtcccgg
     1381 gcaatggcgc caatcaggaa ccagcgcacg cccatcctcc gaagcaccgg ctccatatcg
     1441 gcgagtagct ccaacgtagc ctgatcgagt gggacaggcg acgcgtcaga catgatattc
     1501 ctcccgaatc
//
LOCUS       DAOTON010000763         1510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_65997_length_1510_cov_3.094158, whole genome shotgun sequence.
ACCESSION   DAOTON010000763 DAOTON010000000
VERSION     DAOTON010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65997_length_1510_cov_3.094158"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1510
                     /locus_tag="P5292_12510"
     CDS             <1..>1510
                     /locus_tag="P5292_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39994.1"
                     /translation="VWNLEPNARWSFAQNPDCITPPLFQITECAPDSLHWQHWYDYVH
                     AIVERYDGDGTDDMPGLVLPVRYYVMEQEAYFGGAANGIAGDSGEVRGYGYWEDNVHN
                     LLRLHEVAYQALQDADPSGNTKLIGSGGLLFDLYGDFPDYPDPDGPVVQARLAGNNLM
                     SADYRHGWDSLKTLLRGLAVDTPLRRCDYIGWHPHLGWKSTDQCLRLIRQEALGKPVF
                     IDDMWSAMLTTTLPNGGFTQFIGGDSLERDFPNATVGSYAALINGLDAGDSTVIDWYN
                     AHTAREIVKCFSLCFGEGVERASCSMANDANPNHTLLWPFTGPYRYTGLHASAPNGFA
                     AKPAAYALQLLCDQLHDFTSASPLVVSADPYTRAYRFERQRGTACVVAWSESAQDPAD
                     PHLPNGESVSIGFATDSIILHHPVVTAGTTVHDSTVMGLAVRTWNGTLGFEPVLLEEV
                     GGSTGIGELTQPRLLLAPNPAQEVVRVSSSNNAMIRTITLSGADGRAIGLPAG"
BASE COUNT          293 a          475 c          460 g          282 t
ORIGIN      
        1 tggtctggaa cctggaaccc aacgcgcgct ggtcgttcgc gcagaatccg gattgtatca
       61 cccctcccct cttccagatc accgagtgcg cgcccgactc cctgcactgg cagcactggt
      121 acgactatgt acacgcgatc gtggaacgct acgacggcga cggaaccgac gacatgcccg
      181 gactggtact gccggtgcgg tattacgtga tggaacagga agcctacttc ggcggggcag
      241 ccaacgggat cgccggcgac agcggcgagg tgcggggtta cggctattgg gaagacaacg
      301 tacacaacct gttgcgcttg cacgaagtgg cgtaccaggc cctgcaggat gccgatccgt
      361 cgggcaatac caaactcatc ggcagcggcg gattgctctt cgatctctac ggcgattttc
      421 ccgattaccc cgatcccgat ggtcctgtcg tacaggcgcg gctggccggc aacaacctga
      481 tgagcgcgga ctaccgccac ggctgggaca gtctgaagac cctgttgcgc ggactggcgg
      541 tcgatactcc gctcaggcgt tgcgactaca tcggctggca tccgcatctg gggtggaagt
      601 ccaccgatca gtgcctgcgc ctcatccgac aggaagcgct gggaaaaccg gtcttcatcg
      661 acgatatgtg gtcggcgatg ctgaccacga ccttgccgaa tggcggcttc acccagttca
      721 tcggcggcga ttcgctggaa cgcgatttcc cgaacgcgac ggttggtagt tatgctgcgc
      781 tgatcaacgg actcgatgcc ggcgacagta cggtgatcga ctggtacaac gcccacaccg
      841 cgcgcgagat cgtgaaatgt ttcagtctgt gtttcggaga aggagtggaa cgcgcttcct
      901 gttccatggc gaacgatgcc aacccgaacc acaccctgct gtggcccttc accggaccgt
      961 atcgttacac gggattgcac gccagcgcgc cgaacggttt cgcggccaag cctgccgcct
     1021 acgccttgca actcctctgc gaccagttgc acgattttac gagcgcgagt cctcttgtgg
     1081 tgagcgccga tccttacacc cgcgcctatc gcttcgagcg acagcgcggc acagcctgtg
     1141 tggtggcctg gagcgaatcg gcgcaggacc cggccgatcc gcacctgccg aacggcgaat
     1201 cggtcagcat cggcttcgcg acggattcga tcatcctgca ccatccggtc gtgacagccg
     1261 gcacgaccgt acacgacagc acggtaatgg gtctcgcagt gaggacctgg aacgggaccc
     1321 ttggatttga gccggtgcta ctcgaagaag ttggcggcag caccggtatc ggggaactca
     1381 cgcagccacg cttgttgctc gcgccgaatc cggcgcaaga ggtcgtacgc gtcagcagtt
     1441 ctaacaatgc catgatccgc accataacct tatcgggagc cgatggtcgc gcgatcggtt
     1501 tacccgccgg
//
LOCUS       DAOTON010000764         1510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_66021_length_1510_cov_2.145017, whole genome shotgun sequence.
ACCESSION   DAOTON010000764 DAOTON010000000
VERSION     DAOTON010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66021_length_1510_cov_2.145017"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="P5292_12515"
     CDS             <1..1136
                     /locus_tag="P5292_12515"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRS39995.1"
                     /translation="NAWLQFRDHPAFGAGVSNYRMLQAQYGVGGTPHLNSGHVRFEHP
                     HNEYLLLLAEQGLPGLLLWLAMITALLVPAWRLMKDDQATSPARQAARWSVTGVAGFL
                     VLSMLAYPALRPMERMLFQLHAAILIVVVPRTIGTNLSLKAGRRGWWLLALFFLGSFG
                     VLWQRLGSELHFFQSLVWQAKRQPERQIREVRKAAGYWMQVDFTTTPLIWYEGTHAFY
                     RGEVAKALPYFERSAQLSPWHLRVINDLGTCYEQLGKRDSAVSLYERGLRITPHFVEG
                     RLNLAAAWFNAGRAWQAAATLAKLDPATLRKAEREKYTTYARVILSAIARSDMNAVRD
                     TARYFPWIEGFQGWQEVLIGSKGDSANLKRAIESAWKQAEARQ"
     gene            complement(1130..>1510)
                     /locus_tag="P5292_12520"
     CDS             complement(1130..>1510)
                     /locus_tag="P5292_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39996.1"
                     /translation="PGVCNIGQKEVSVRWKFIRLFLPVTAFLSIGSFYWWDSLWVWFF
                     LLCSTFSLLVLCWEVRTRFCIIFGFFSLHNFRHLGELEEVENPDDIRRDRKRVWEITL
                     QAMAIALGYATSVHLLASYLGLHH"
BASE COUNT          313 a          422 c          480 g          295 t
ORIGIN      
        1 gcaacgcctg gttgcagttc cgggatcatc cggcgttcgg cgcgggtgtt tcgaattaca
       61 ggatgttgca ggcgcaatac ggagtcggtg gtacgccgca cctcaacagc gggcacgttc
      121 gctttgagca tccgcacaac gaatacctct tgttgctggc ggaacaaggc ttgccgggtc
      181 tcctgctctg gctggccatg atcacggccc tgctcgtgcc ggcctggcgt ttgatgaaag
      241 atgaccaggc aacttccccg gcccgacagg cagcccgttg gagcgtgacc ggagtagcgg
      301 gtttcctggt gttgtcaatg ctggcgtatc cggccctgcg accgatggag cggatgctgt
      361 tccaactgca cgcggcgatc ctgatcgtcg tggttccgcg gaccatcggc acgaacctat
      421 cgttgaaggc gggcagacgc ggctggtggt tgctcgcctt gtttttcctc ggctcgttcg
      481 gcgtgctctg gcagcggctg ggatccgagt tgcacttttt tcagtcgctc gtctggcagg
      541 cgaagcgtca gccggagcga cagatccggg aggtgcgcaa ggccgcggga tactggatgc
      601 aagtggactt tacgaccacg ccgctgatct ggtacgaagg cacccacgcg ttctatcgcg
      661 gagaagtggc gaaggccttg ccctatttcg agcggtcggc acaactatcg ccctggcatt
      721 tacgggtcat caacgatctc gggacttgct acgagcagtt gggcaagcgc gacagtgcag
      781 taagcttgta cgaacggggg ttgcgcatca caccgcattt cgtcgaggga agattgaatc
      841 tcgccgcggc ctggttcaat gccggtcgtg cctggcaggc tgcggccacc ttggcaaaac
      901 tcgatcctgc tacgctccgg aaagcggaac gggagaagta cacgacctac gcgcgtgtca
      961 tcctcagcgc gatcgcccgt tccgatatga atgcggtacg cgataccgcg cgctattttc
     1021 catggatcga aggcttccag ggatggcagg aggtgttgat cggaagcaaa ggagattcag
     1081 cgaacctgaa gcgggctatc gaatcagcct ggaagcaggc ggaagcgcgt cagtgatgca
     1141 gccccagata ggaggcgagt aaatgaaccg aggtggcgta gcccagtgcg atcgccatcg
     1201 cctggagggt gatctcccag acccgtttgc gatcgcgccg gatgtcgtcg ggattctcca
     1261 cttcttccag ttcgccgagg tgacggaaat tgtgcaggct gaaaaatccg aagatgatgc
     1321 agaagcgggt acgcacctcc cagcatagta ccagcaggga gaacgtggag cacagcagga
     1381 agaaccagac ccaaagcgag tcccaccaat aaaagctgcc gatggacaag aaggccgtca
     1441 ccggcagaaa aagccgaatg aatttccagc ggacggaaac ctctttctgt ccgatgttgc
     1501 atacacccgg
//
LOCUS       DAOTON010000765         1509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_66052_length_1509_cov_4.303989, whole genome shotgun sequence.
ACCESSION   DAOTON010000765 DAOTON010000000
VERSION     DAOTON010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66052_length_1509_cov_4.303989"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="P5292_12525"
     CDS             complement(<1..866)
                     /locus_tag="P5292_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS39997.1"
                     /translation="MIAPRPFLFPILLVCIGLLVACKAPPQPGSIPYDLDHPREVRSL
                     PKALKEISGIVSLDQRRLLCVEDEKGEVFVLGRKKDDIKHAVGFGKDHDYEGISLVND
                     TVFVLKSNGTLFRVTGWTTDTPQTQAIHTFLKKENNTEGVCYDAANRRLLIACKGVAG
                     HGPLERLKAVYAFDLARQQLLETPVLLLDPDSVAAYLSKYHVKAMHAAVDGSTIPGLE
                     LFAPSDLAIDPATGNLYLLCSTGKLLLVFDPADRLIFASPLDPSIYLQPEGITFSPDG
                     ELLISNEGRGGKA"
     gene            1037..>1509
                     /locus_tag="P5292_12530"
     CDS             1037..>1509
                     /locus_tag="P5292_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRS39998.1"
                     /translation="MKFKRVTDLLRTEAADQTVEVRGWVRTRRGNKQIAFIAVNDGSV
                     IHSIQVVAEMAKFPEELIKRVTTGACIRATGKLVASQGSGQAVEIQAETIEILGDADE
                     SYPLQKKGHTLEFLREIAHLRPRTNTFGAVFRLRHHMAFAIHQYFNDRGFFYLHSP"
BASE COUNT          330 a          399 c          471 g          309 t
ORIGIN      
        1 gctttgccgc cccggccttc gttggagatc agcaattcac catcggggga aaaggtaatg
       61 ccttcgggtt gcaggtagat ggatggatca agcggcgaag cgaagatgag ccgatctgcc
      121 ggatcaaaaa cgagcagcag cttaccggtg gagcacagca ggtagagatt gccggtggct
      181 ggatcgatgg cgagatcgga aggggcgaac agttcgagtc cgggaatggt ggagccgtcg
      241 accgccgcat gcatggcttt gacatggtac ttactcaggt aggcggcgac ggaatccggg
      301 tcgagcagta acaccggtgt ttcgagtagc tgttgccgcg caaggtcgaa ggcatagacg
      361 gctttcaaac gctcgagcgg accgtgaccg gcgacgcctt tgcaggcgat gagcaggcga
      421 cgattggcgg catcgtaaca aacgccttcg gtgttgtttt ccttcttgag aaacgtatgg
      481 atcgcttgtg tttgcggcgt gtcggttgtc cagcccgtga cgcgaaagag cgtaccgttg
      541 cttttgagga cgaagaccgt gtcgtttacg aggctgatgc cttcgtagtc gtggtcctta
      601 ccaaaaccga ccgcgtgttt gatgtcgtcc ttctttcttc ccaggacaaa gacctcccct
      661 ttctcgtctt cgacacagag cagtctgcgt tgatcgagcg aaacgatgcc ggagatctcc
      721 ttcagggctt tcggcaggga gcggacttcc cggggatggt cgaggtcgta gggaatggag
      781 ccgggttggg gcggcgcttt acaggcgacc aggagtccga tacaaacgag aaggatggga
      841 aaaagaaaag gccgcggtgc aatcacaggc cgcgaagtac cccttttcaa attaattcgg
      901 gatgaaacga agggctaaca aactcaaaaa gcgatttttc gccccgaaaa accacctgaa
      961 actcaatatc cgggcaagct tccgccttgc tcctcccccc gcgaatcgct tattttgcag
     1021 cctcatttct actgccatga agtttaaaag ggtaacggat ctgttacgaa cggaagcggc
     1081 cgatcagacg gtcgaagtca ggggctgggt caggacccgg cgcgggaaca agcagatcgc
     1141 gttcatcgcg gtgaacgacg gttccgtgat ccacagcatc caggtggtcg ccgagatggc
     1201 gaagtttccg gaggagttga tcaagcgcgt gaccaccggc gcctgcatca gggcgacggg
     1261 caagctggtc gcttcgcagg gatcggggca ggccgtggag atccaggcgg agacgatcga
     1321 gatcctcggt gacgcggatg agtcgtatcc gttgcagaag aaaggacata cgctcgagtt
     1381 cctgcgcgag atcgcgcact tgcgtccgcg tacgaacacc ttcggcgccg tcttccggct
     1441 gcgtcaccac atggcgtttg ccatccacca gtacttcaac gaccgtggtt tcttttacct
     1501 ccactcccc
//
LOCUS       DAOTON010000766         1508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_66141_length_1508_cov_2.607708, whole genome shotgun sequence.
ACCESSION   DAOTON010000766 DAOTON010000000
VERSION     DAOTON010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66141_length_1508_cov_2.607708"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="P5292_12535"
     CDS             complement(<1..1086)
                     /locus_tag="P5292_12535"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRS39999.1"
                     /translation="MSASSGRQNVIIFIFLSVGLVYLMRLFFLQVLDNSYKNSADNNV
                     IRIITDYPSRGLIYDRKGRLLVYNEPVYDLMIIPKQARNLDTLELCGLIGITKEDYIA
                     KFQKARAYSPVKPSLFEKQLSVETYATLQEKLYKFPGFFVEPRTLRKYPKPVAAHLFG
                     YIGEVDSATTRKNPYYKEGDYIGKSGVELSYEEALRGKRGSHRVMVDVFNREKGKFQN
                     GRFDTVAVAGENLTLTIDAKLQEYGEQLLANKVGSVVAIEPATGEILCMVSSPAYDPN
                     LLVGRSRTKNYGRLLVDPFKPLFNRSQMALYPPGSTFKLVMALLAQQDGVLFPTTRYP
                     CAHGYPPMGGKPKCHPHASPLDLQWSIT"
     gene            complement(1067..>1508)
                     /locus_tag="P5292_12540"
     CDS             complement(1067..>1508)
                     /locus_tag="P5292_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRS40000.1"
                     /translation="VNPYVYILFLLLLPVETPKALQLVLGLVTGLVIDMFGNTSGLHA
                     AASVFLMFARPGILRLIAPRDGYDAEVRLIPQVMGLNWFLTYAGILTFLHHLLYFYLE
                     VFRFTEFGMTFLKVVINSAVSLLLIVMGTYLFIRSGKSNERILG"
BASE COUNT          373 a          410 c          410 g          315 t
ORIGIN      
        1 tgtgatcgac cactgcagat caaggggaga cgcgtgcggg tgacacttgg gttttccgcc
       61 catcggcggg tacccgtgcg cgcaagggta acgggtcgtg gggaacaaga caccgtcctg
      121 ctgcgccagc agggccatca ccagtttgaa ggtcgaaccc ggcgggtaca aggccatctg
      181 ggagcggttg aagagcggct tgaacggatc aaccaacagc ctgccgtaat tcttggttcg
      241 cgaccgaccg accagcaaat tcggatcgta agcaggtgag ctgaccatgc agaggatctc
      301 gccggtagca ggctcgatcg ctaccacgga acccactttg ttcgcgagca attgctcacc
      361 gtactcttgc agcttggcgt cgatggtcag ggtcaggttc tctcccgcca ccgcgacagt
      421 atcgaaccga ccattctgga attttccctt ctctcggttg aatacatcca ccatcacacg
      481 atgggatccg cgcttcccgc gaagggcttc ttcgtacgac agctccacac cgcttttccc
      541 gatataatca ccctctttgt aataagggtt cttacgggtc gtggcggagt cgacttcacc
      601 gatatatccg aacaaatgcg ccgcgaccgg tttgggatac ttgcgcaagg tgcgcggctc
      661 cacgaagaac cccgggaact tgtacaactt ctcctgaagc gtcgcgtagg tctcgaccga
      721 taattgcttt tcaaacaacg aaggcttcac cggcgaatag gctctggcct tctgaaactt
      781 ggcgatgtaa tcttccttcg tgatgccgat caaaccgcac aattccagag tatccagatt
      841 tcgggcctgc ttcggaatga tcatcaggtc atagaccggt tcattgtaga caagcaatcg
      901 tcccttgcgg tcataaatca aaccgcgtga tggataatcc gtgatgatcc ggatgacgtt
      961 gttgtccgct gaattcttat aggaattgtc gagcacctgc agaaagaaca ggcgcatgag
     1021 gtacaccagt ccaaccgaca ggaaaatgaa gatgatgacg ttctgtctac ccgaggatgc
     1081 gctcattgga ttttcccgaa cggatgaaca ggtaggtgcc catgacgatg agcaggagcg
     1141 aaaccgcgga gttgatcacc accttcagga aggtcatgcc gaactcggta aagcgaaaga
     1201 cctccagata gaaatacagg aggtggtgca ggaaggtgag aatacccgcg taggtcaaaa
     1261 accagttcaa tcccatcacc tggggaatca accggacctc ggcatcatac ccgtcccgtg
     1321 gtgcgataag gcgtaagatc cccggacggg cgaacatcag aaacacgctc gccgcggcgt
     1381 gtaaaccgct ggtatttccg aacatgtcga tgaccagtcc ggttacgagc ccgagtacga
     1441 gttgcagagc cttgggagtc tcgaccggca acaacaaaag gaataatata tatacatacg
     1501 ggttcacg
//
LOCUS       DAOTON010000767         1505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_66306_length_1505_cov_4.855862, whole genome shotgun sequence.
ACCESSION   DAOTON010000767 DAOTON010000000
VERSION     DAOTON010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66306_length_1505_cov_4.855862"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="P5292_12545"
     CDS             complement(<1..842)
                     /locus_tag="P5292_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRS40001.1"
                     /translation="MSSSSLNKVTVGGLLVTLGIIFGDIGTSPLYVLKAIVGKEAISE
                     ETVLGGISCIFWTLTLQTTIKYVILTLRADNKGEGGIFSLYTLVRRTKVPWLVFPTII
                     GGAALLADGVITPSISVSSAIEGLRYFDPAINTVPIVIAILTALFLIQQFGTKFVGRF
                     FGPVMFTWFLMIGVLGILSIVHAPHVLAAVNPLYAYRLLAEHPNGFWLLGAVFLCTTG
                     AEGLYSDMGHCGRKNIRASWGFVKVMLLLNYFGQAAWLVGQEGAFLEDRNPFYSMMPD
                     SFRW"
     gene            999..>1505
                     /locus_tag="P5292_12550"
     CDS             999..>1505
                     /locus_tag="P5292_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRS40002.1"
                     /translation="MHPTYDVIVVGAGHAGCEAAAAAANMGSSVLLVTMNLQTIAQMS
                     CNPAMGGIAKGQIVREIDALGGYSGIVSDRSMIQFRMLNRSKGPAMWSPRTQNDRMLF
                     AAEWRRLLEQTPNVDFWQDMVNGLIINNNRVVGVRTGMGQEFRAKAVVLTNGTFLNGQ
                     PIKRQRLHN"
BASE COUNT          360 a          402 c          430 g          313 t
ORIGIN      
        1 atccagcgga agctgtcggg catcatcgaa taaaacgggt tgcggtcttc gaggaaggct
       61 ccctcctgcc ctaccagcca ggcagcctgc ccgaagtagt tcagcaacaa catcactttt
      121 acgaagcccc agctcgcccg gatatttttg cggccgcagt gacccatgtc ggagtacagg
      181 ccttctgcac cggtagtaca aaggaagact gcgcccagca accagaatcc attcgggtgt
      241 tcggccagca gccgataggc gtagagcgga ttgaccgctg ccagcacatg cggcgcgtgc
      301 acgatcgaca gtatcccaag cacgccgatc atgaggaacc aggtgaacat caccggaccg
      361 aagaagcggc cgacgaactt ggtgccgaac tgttggatga gaaataatgc cgtgaggatc
      421 gcaatgacga tcggtaccgt gttgatcgcg ggatcgaagt agcgaagccc ctcgatggcg
      481 gaagaaaccg aaatggaagg tgtaatgact ccgtctgcaa gcagcgcagc gccgccgatg
      541 attgttggaa acaccagcca cggtactttc gtgcgacgca ccaatgtata aagtgagaag
      601 atgccgccct cacccttgtt gtccgcgcgc agcgtcagga tcacatactt gatcgtggtc
      661 tgcagggtaa gcgtccagaa gatacaggag atcccgccga gcacggtctc ttcgctgatg
      721 gcctccttgc cgacaatggc tttcagcaca tacagcggcg acgttccgat gtcgccgaaa
      781 atgattccga gcgtaaccag taggccaccg acggtaacct tgttaagcga agaagaactc
      841 aaagcctggc gttaggttcg caaaggtagc aaaggacgta ctccttcatt ctttcgcgca
      901 atgattcacg tggaacatga aatcagttcg aaaggttgcg ctaagtggct ggtggactgg
      961 ggaattctta ttacttacct ttgcggcccc gaatgtcact gcacccgaca tatgatgtta
     1021 tcgtagtagg cgccggccat gccggttgcg aagctgcggc agctgctgcc aacatgggct
     1081 ccagcgttct gctggtcacc atgaacctgc agaccattgc ccagatgtcg tgcaacccgg
     1141 ccatgggcgg aattgctaaa ggtcagattg tcagggaaat agatgcgctc ggcggttatt
     1201 ccggcatcgt ttccgatcgt tccatgattc agtttcgcat gctgaacagg tcgaaggggc
     1261 ctgccatgtg gagtccgaga acacagaacg atcgtatgct tttcgctgct gaatggcggc
     1321 gcttgctgga gcaaactccg aacgtggatt tctggcaaga tatggttaat ggcctgatta
     1381 tcaataataa tcgggtcgtt ggcgttcgta ccggcatggg gcaggagttt cgcgccaaag
     1441 cggtggtgtt aaccaatgga acctttctga atggccaacc catcaagcgc cagcggctgc
     1501 acaac
//
LOCUS       DAOTON010000768         1505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_66321_length_1505_cov_2.884828, whole genome shotgun sequence.
ACCESSION   DAOTON010000768 DAOTON010000000
VERSION     DAOTON010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66321_length_1505_cov_2.884828"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="P5292_12555"
     CDS             <1..224
                     /locus_tag="P5292_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40003.1"
                     /translation="LTNQGIYRFDPTALTLQQESSLSGDRMVRDSDSQLIYLIDSLEV
                     KLLSDIDLSVTGTITSTEPVKNVINWYNR"
     gene            298..1254
                     /locus_tag="P5292_12560"
     CDS             298..1254
                     /locus_tag="P5292_12560"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRS40004.1"
                     /translation="MNFLDFEKPISDLLDQIDKQKQIGEKSKVDVSVAVRQLELKLEE
                     TRKVIYSKITPWQRVQLSRHPDRPYALAYIESITEKNFIEQFGDRTVRDDKAIVGGFG
                     SVDGQTVMFIGQQKGATTKQRQYRNFGMANPEGYRKALRLMKLAEKFNKPVVTFIDTP
                     GAYPGLEAEERGQGEAIARNLFEMAKLKVPVICIIIGEGASGGALGIGIGDRVLMLEN
                     TWYSVISPESCSSILWRSWDYKEKAAEVLKLTAEDMLKNKLIDGIIREPIGAAHTQPE
                     QMFRIVKAEIKKHLADLLAKDRATLVHERIEKFCSMGVVAEQ"
     gene            1318..>1505
                     /locus_tag="P5292_12565"
     CDS             1318..>1505
                     /locus_tag="P5292_12565"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046239.1"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRS40005.1"
                     /translation="MISFPNCKINLGLHVLSRREDGFHELDTVFYPVPWTDILEIVRD
                     EGNESDIDFRNSGIRVYGP"
BASE COUNT          382 a          415 c          388 g          320 t
ORIGIN      
        1 tgctcaccaa ccagggcatc tatcgcttcg atcctactgc gcttaccctt caacaggaat
       61 cctccctttc cggtgatcgc atggtccggg atagtgatag ccagttgatc tacctgatcg
      121 attcgctgga ggtgaaactt ctttccgaca tcgacctttc cgtgaccgga acgatcacca
      181 gcaccgagcc cgtgaaaaac gtgatcaact ggtacaaccg ctgaaatccg gcgctttgta
      241 tattcgtagt gcaatgacaa gcgaactgaa ttgcaagtcg ttgattaaaa gcttattatg
      301 aacttcctcg actttgaaaa accgatctcg gaccttctgg accagatcga caagcagaag
      361 cagatcggtg aaaaaagcaa ggtggatgtc agcgtagccg tacgccagtt ggaattgaaa
      421 ctggaagaaa cccgcaaggt catctatagc aagatcacac cctggcaacg tgttcagttg
      481 tcacgccacc ccgaccgccc ttacgccctg gcctacatcg agtcgatcac tgagaagaat
      541 ttcatcgaac aattcggcga tcgtaccgtg cgggacgata aggccatcgt gggcggcttc
      601 ggttcggttg acggacagac cgtcatgttc atcggacaac agaagggtgc cactaccaag
      661 caacggcaat accgcaactt cggcatggcc aacccggaag gataccggaa agcgcttcga
      721 ctcatgaagc tggctgaaaa gttcaacaaa ccggtcgtta ctttcattga tacgccggga
      781 gcatatcccg gtctggaagc tgaagaacgg ggccagggcg aagccattgc ccgcaacctg
      841 tttgagatgg ccaaactcaa ggtaccggtc atttgcatca tcatcggcga gggagcttcc
      901 ggtggcgcgc tcggcatcgg catcggcgac cgcgtactga tgctggagaa tacctggtac
      961 tcggtcatct caccggaatc ctgttcctct attctctgga gaagttggga ctacaaggaa
     1021 aaagccgcgg aggtcctgaa actcaccgcg gaggatatgc tgaagaacaa actgatcgac
     1081 ggcatcatcc gggaaccgat cggtgcggca catacccaac ccgaacagat gttccggatt
     1141 gtcaaagctg aaatcaagaa gcacctggcc gacttactcg ccaaggatcg cgcgaccctg
     1201 gtccacgaac ggattgaaaa attctgttcg atgggcgtgg tagcagaaca ataatggcag
     1261 gatggcgcaa ccggtacacc atacccgctg ctcctctccg gcgtttcgca tcgccccgtg
     1321 atcagctttc ctaattgcaa gatcaacctg ggcttgcacg tcctttcccg tcgtgaagat
     1381 ggtttccacg aactggatac ggtcttttat cctgttccct ggacggatat cctggagatc
     1441 gtacgcgacg aaggaaatga atccgacatc gatttccgca acagcggcat tcgcgtctat
     1501 ggacc
//
LOCUS       DAOTON010000769         1503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_66451_length_1503_cov_2.205110, whole genome shotgun sequence.
ACCESSION   DAOTON010000769 DAOTON010000000
VERSION     DAOTON010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66451_length_1503_cov_2.205110"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="P5292_12570"
     CDS             complement(<1..762)
                     /locus_tag="P5292_12570"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HRS40006.1"
                     /translation="MKLRNIYFTLIFSTLTIVTFGQATDVNKISAITGDTLKGAAASD
                     TSWKTGGFIGINFSQISLSHWAPGGDNSISIAANANVFANYAKNRTQWDNNLLLAYAL
                     LKNGTDPLRKNDDRIDFTSKFGYRVKPESKWFYSAILNFKSQVANGYNYPNDSVVISR
                     FLAPAYLTLALGMNYKPVDYFEVFFSPVSSRFVIVNDQDLADLGAYGVKAATTDANGN
                     LNKGTGKKVRSEFGAYLNMNFKKEIFENVTLASRLE"
     gene            complement(847..1260)
                     /gene="mscL"
                     /locus_tag="P5292_12575"
     CDS             complement(847..1260)
                     /gene="mscL"
                     /locus_tag="P5292_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRS40007.1"
                     /translation="MGLISEFKDFINKGNVIDLAVGVVIGGAFGKIVNSLVSDIIMPP
                     IGLLIGGVNFSEIKLTIKEAAVDAAGKAIPAVSINIGNFIQVLFEFTIIAFAIFMVVK
                     AVNRMRKADEPAPAPPPGPTKEEVLLTEIRDLLKK"
     gene            1370..>1503
                     /locus_tag="P5292_12580"
     CDS             1370..>1503
                     /locus_tag="P5292_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS40008.1"
                     /translation="MITLHQVRFAFGGRYLLDGASWQINPGEKIGLIGRNGTGKSTLL
                     R"
BASE COUNT          340 a          344 c          436 g          383 t
ORIGIN      
        1 ttcgagacgg gaggcaaggg tcacattctc gaagatctct ttcttgaagt tcatgttgag
       61 gtaggcgccg aactcggatc gtactttttt gccggtgcct ttgttgaggt tgccgttggc
      121 gtcggtagtc gcggccttta cgccataggc gccgagatcg gccaggtcct ggtcgttcac
      181 gatgacgaat cgggaggaaa ccggggagaa gaatacctca aaatagtcga ccggcttgta
      241 gttcataccg agcgcaagcg tcaggtaggc tggcgcgaga aatcgggaga tgaccaccga
      301 gtcgttcggg tagttataac cgttcgctac ctggctcttg aagttgagga tggcactgta
      361 gaaccatttg ctctccggct tgacgcgata accgaatttg gaggtaaagt caatgcggtc
      421 gtcgttcttt cgcaaaggat cggtgccgtt cttcaacaaa gcataggcaa ggagcaggtt
      481 attgtcccat tgggtacggt tcttcgcgta gttggcgaaa acgttggcat tggcggcgat
      541 ggagatggaa ttatcgccac ccggtgccca gtgggaaagg ctgatctgag agaagttgat
      601 gccaatgaat ccgccggtct tccaggaggt gtcggaggca gcagctcctt ttaaggtgtc
      661 gccggtgatg gccgagatct tgttaacgtc ggtagcctga ccgaaggtaa cgatggttaa
      721 ggtgctgaat atcagcgtaa aatagatgtt tcgcagtttc attctttcgt tggttttagc
      781 gggtgcaaag aaaagcaaaa aggaggcggt ggacattccc aacgcctcct tttctactga
      841 tgttgatcac tttttaagca ggtcccggat ctcggtcagg agtacttcct ctttagttgg
      901 tccgggaggt ggagcgggag ctggttcatc ggccttgcgc atccggttga cagccttgac
      961 gaccatgaag atcgcaaagg cgatgatggt gaattcgaac aatacctgga taaagtttcc
     1021 gatgttgatg ctgacagccg ggatggcttt accggcggcg tccaccgcgg cttccttgat
     1081 cgtcagcttg atctccgaaa aattaactcc accgatcagc agcccgatgg gcggcatgat
     1141 gatatccgac accaacgagt tgacgatctt cccaaaggct ccgccgatca cgacaccgac
     1201 ggccaggtcg atcacgtttc ctttgttgat gaaatccttg aattctgaaa tgagtcccat
     1261 ggttgtggtg ttttggtcag gttgaaagta agaagtcttt ttatgcgagt caaatcagcg
     1321 gcttgaagta tcgttatgca taacgcctaa atattcggaa attcgcggcg tgattaccct
     1381 gcaccaagtc cgcttcgctt tcggtggacg ttatttgctc gatggcgctt cctggcagat
     1441 caatcccggt gaaaagatcg gactcattgg acgaaacggg accggtaaat cgaccttgtt
     1501 acg
//
LOCUS       DAOTON010000770         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_66543_length_1501_cov_3.621715, whole genome shotgun sequence.
ACCESSION   DAOTON010000770 DAOTON010000000
VERSION     DAOTON010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66543_length_1501_cov_3.621715"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1501)
                     /locus_tag="P5292_12585"
     CDS             complement(<1..>1501)
                     /locus_tag="P5292_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRS40009.1"
                     /translation="LIGYTSFAQILIRSNANPPLDENNPENVFSFISYLKREQYGDRP
                     LFYGQFYNAKVVDQKEGERTWAKVPGESKYVDAGRKVEAVYEADKTTIFPRMWSNQAN
                     HVQEYKKWADVKGDRTPSFGENLKFFFVYQLGEMYWRYFMWNFVGRQNDLQGPGGITK
                     GNWLSGIKFLDEMRLGPQDKLPESMTNNRARNTLYFLPFILGIIGLIYHFNRDNKDAW
                     VVFLLFFMTGIAIVIYLNGTPQQPRERDYAYAGSYYAYAMWIGIGVLAIYDFLRKRIP
                     GTVAAFTSTLLCTFAVPVVMAKAEWNDHDRSNRYTSRDFAVDYLESCERNAILFTNGD
                     NDTFPLWYAQEVEGVRTDVRVVNLSLLNTDWYIDQLRRKYYDSDPINISWTQDKYILG
                     RRDYVPFYDRGMTEPIELKKLVDFMGSDDPAAKARTQGGEMINYYPGKRIKLTIDSAA
                     VMANGAVSKEDADKIVKEMVWDVSGNYLMKNDLMILNILANNNWKRPIYF"
BASE COUNT          295 a          409 c          451 g          346 t
ORIGIN      
        1 cgaaatagat cgggcgcttc cagttgttgt tggccaggat gttgaggatc atgagatcgt
       61 tcttcatcag gtagttgccc gaaacgtccc acaccatctc cttcacgatc ttatcggcgt
      121 cttccttcga tacggcgccg ttcgccatca ccgcggcgga gtcgatcgtg agcttgatgc
      181 gttttccggg atagtaattg atcatctcac cgccctgggt gcgtgccttg gcggccggat
      241 cgtcgctgcc catgaaatcc acgagcttct tcagttcgat cggttcggtc atgccgcgat
      301 cgtagaacgg aacgtaatcg cgacggccca ggatgtactt gtcctgcgtc caactgatgt
      361 tgatcgggtc ggaatcgtaa tacttccgtc ggagctggtc gatgtaccag tcggtattca
      421 gcaggctcag gttgacgacc cgcacatccg tgcgcacgcc ttctacctcc tgggcgtacc
      481 agagcgggaa ggtgtcgttg tcgccgttgg tgaacaggat cgcgttgcgt tcgcaggatt
      541 cgaggtaatc gacggcgaag tcgcgcgagg tataacggtt cgaacggtcg tggtcgttcc
      601 attcggcttt ggccatgacc accggcacgg cgaaggtgca gagcagggtg gaggtgaagg
      661 ccgccaccgt acccggaatg cgttttcgca aaaagtcgta gatcgccagc acgccgatac
      721 cgatccacat cgcgtaggcg tagtaggagc cggcataggc gtaatcacgc tcgcgcggct
      781 gttgcggagt accgttgagg taaatgacga tcgcgatgcc ggtcatgaag aacagcagga
      841 acacgaccca ggcgtctttg ttgtcgcggt tgaagtggta gatcagtccg atgatgccga
      901 ggatgaacgg caggaagtac agggtgttgc gcgcccggtt gttggtcatg ctttcgggca
      961 gcttgtcctg cggaccgaga cgcatttcgt cgaggaactt gatacccgaa agccagttgc
     1021 ctttcgtgat gccgccggga ccctgcagat cgttctgacg tccgacgaag ttccacatga
     1081 aataacgcca gtacatctcg cccagttgat agacgaagaa gaacttcagg ttctcaccga
     1141 agctcggcgt gcggtcgcct ttgacatcag cccacttctt atactcctgt acgtggttgg
     1201 cctggttgct ccacatgcgc gggaagatgg tggtcttgtc ggcttcgtac accgcttcca
     1261 ccttgcggcc ggcatccacg tacttcgact cacccggcac cttggcccag gtgcgctcgc
     1321 cttctttctg gtcaacgact ttcgcgttgt agaactgacc atagaacagc ggacgatcgc
     1381 cgtactgctc acgcttcaga taagagatga agctgaaaac gttttccgga ttgttctcgt
     1441 cgagcggcgg gttggcgttc gagcggatca ggatctgcgc gaacgaggtg taaccgatga
     1501 g
//
LOCUS       DAOTON010000771         1498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_66737_length_1498_cov_2.415107, whole genome shotgun sequence.
ACCESSION   DAOTON010000771 DAOTON010000000
VERSION     DAOTON010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66737_length_1498_cov_2.415107"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="P5292_12590"
     CDS             complement(<1..1195)
                     /locus_tag="P5292_12590"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRS40010.1"
                     /translation="MARIIALAFVLLLALLSSCGKRKADVLYFNGTIYSGESSSSNLQ
                     AFAVRDGRIVSTGSDDDILDGFDAPKKVDLKGRTVLPGFIDAHCHFFGYASDLLKCDL
                     TGSRSYEAALELLNAFSKTNKFSWLLGRGWDQNDWEDHSFPTRERLDSLYPTVPVFLM
                     RIDGHAALCNAVALQLAGINGDTRVAGGEVLLNQSGEPTGLLIDNAVDLVKAKIPPFP
                     DSLNAEALLKAQQHCFSVGLTTVDDAGLGKDSIFLIDRLQQEGKLKLRVYAMISDSKE
                     TRDYFFSKGPYKTDRLNVRAVKVYADGALGSRGACLKRPYADQPGHYGFLLHDLAYMN
                     DIMDEAHENGFQVCTHAIGDSAVKTILMLYRENLSGENPRRWRIEHCQVVDPADMDYF
                     SRYSII"
     gene            1256..>1498
                     /locus_tag="P5292_12595"
     CDS             1256..>1498
                     /locus_tag="P5292_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018886466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HRS40011.1"
                     /translation="METIEIMRFPIGRFSTPSECTPELRKEWIRIISVTPERLRLLVE
                     GLTDQQLDTPYRDGGWTVRQVVHHLVDSHINSYIRFK"
BASE COUNT          369 a          388 c          403 g          338 t
ORIGIN      
        1 gaatgatcga gtatcggctg aagtaatcca tgtcggcggg atctactacc tggcaatgct
       61 cgatcctcca gcgtcgtgga ttttctcccg ataaattctc ccgatacagc atcaggatcg
      121 tttttactgc ggaatctccg atggcgtggg tgcaaacctg gaaaccgttc tcgtgggctt
      181 cgtccatgat gtcgttcatg tatgcaaggt catgcaacag gaatccataa tgcccgggtt
      241 ggtcggcata cggacgtttc aggcaggcac cccgggaccc caacgcaccg tcggcgtaca
      301 ctttaacggc tcgtacgttc aggcgatcgg tcttataggg acctttggaa aaaaagtaat
      361 cgcgcgtctc cttcgaatcc gagatcatcg cgtagacacg cagtttgagt ttcccttcct
      421 gctgcaggcg atcgatcagg aagatactgt ctttacccag gccggcatcg tcaaccgtag
      481 taagcccgac ggaaaagcag tgctgctgtg ctttcaggag cgcttcggcg ttgagagaat
      541 cggggaaagg tgggatcttg gccttgacca ggtcgactgc attgtcgatc aatagtccgg
      601 taggctcacc ggattgattg agcaatacct cgccgccggc aacccgcgta tccccgttaa
      661 tacctgccag ttgaagcgcg acagcattgc agagcgccgc atgaccatca atgcgcatca
      721 ggaaaaccgg gacagtcggg taaaggctgt caagccgttc ccgggtaggg aaggagtgat
      781 cttcccagtc gttctgatcc cagcctcggc ccaggagcca actgaattta ttcgtcttag
      841 aaaatgcatt caaaagttcc aatgcagctt catacgagcg cgagcctgtc agatcgcact
      901 tcaatagatc acttgcatac ccgaaaaagt ggcagtgagc gtcgatgaac cccggaagga
      961 cggtacgccc tttcaaatcg acttttttag gggcatcaaa gccgtcgaga atgtcatcat
     1021 ccgacccggt actgacgatt cggccgtcac gcactgcaaa ggcctgaaga ttcgaggaac
     1081 tgctttcgcc ggaatagatg gtcccgttga aataaagaac atcggctttg cgttttccgc
     1141 aggaacttag cagggccaac aggagaacga atgcaagagc gataatacga gccattcagg
     1201 agggggttgt agctgcaaag taaaccaata tcttcgcagt aaactcgctt tgccaatgga
     1261 aacaattgaa atcatgcgct ttcccatcgg gcgattttca actccttcgg agtgtacgcc
     1321 ggaattgcgt aaagaatgga tccggatcat ctcggtgacg ccggaacggt tgcgactact
     1381 ggtggaaggc ctgaccgatc agcaattgga taccccttat cgtgacggcg gttggacagt
     1441 ccggcaggtg gtgcatcacc tggtcgacag tcatatcaac agttacatca ggttcaaa
//
LOCUS       DAOTON010000772         1495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_66944_length_1495_cov_2.647222, whole genome shotgun sequence.
ACCESSION   DAOTON010000772 DAOTON010000000
VERSION     DAOTON010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66944_length_1495_cov_2.647222"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="P5292_12600"
     CDS             <1..817
                     /locus_tag="P5292_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRS40012.1"
                     /translation="GLRFVLHQLFEGAVVVAVTWIVLRLVDFFGLVLIERARETEDRS
                     DDQLVPFVKEGIKVILAGVGLLITLAVSFDLDVVSLVTGLGIGGLAFALAAKETLENL
                     LGSFTIFLDKPFRVGDQVKVGPVEGKIESVGFRSTRIRALDRMLVTVPNKKMVDAELI
                     NETEREVRRSRQVFLLEHSTPPERIEEVLTDIRGYIEVQHELESGFIVRLQNVTQHGL
                     ELLVVYIVKTPVFEEFLAVQERIHLEILGILHRHQVRFASTAPVVNLSASPDR"
     gene            complement(821..>1495)
                     /locus_tag="P5292_12605"
     CDS             complement(821..>1495)
                     /locus_tag="P5292_12605"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRS40013.1"
                     /translation="KGMATPDSKRLQQLLKRPLLRSLAQASLPEQGESTFLYRTLRQT
                     VESADYLSAHTNSTHTTGNYPNHAFGKQLRQIASLIGAGVETRFFYVSLPGFDTHALQ
                     KNIQNRLLKTFDQGLSALIHDLKQQDRFYETRILVFSEFGRRVAQNGSNGTDHGAANY
                     VFLAGGNMRRKGLINGLPDLGDLDEGDVKFKTDFRQVYATILEEWLGEDATTILGNHY
                     PTLNIL"
BASE COUNT          294 a          378 c          442 g          381 t
ORIGIN      
        1 cggcctgcgt ttcgtgctgc atcagttgtt cgaaggcgcc gtcgttgtcg ccgttacctg
       61 gattgtgctg cgattggtcg actttttcgg acttgtcctg atcgagcgcg cgcgcgaaac
      121 ggaagaccgg tcggatgatc agttggttcc ttttgtcaag gaaggaataa aggtgatcct
      181 ggcaggtgtg ggcttgctca tcaccctggc ggtctcgttt gatctcgatg tcgtatcgct
      241 ggtcaccgga ctggggatcg gcggacttgc cttcgcgttg gcggcaaaag aaacactgga
      301 gaacctgctg ggttcattta cgatatttct tgataaacct tttcgtgtcg gtgaccaggt
      361 gaaagttggt ccggtggaag gtaagatcga aagcgtagga tttcgttcga cccggattcg
      421 tgccctggac cgcatgctgg tgactgttcc gaacaagaaa atggtcgacg cggaactcat
      481 caacgaaacg gaacgcgagg tgcgcaggtc ccgtcaggtt tttctgttgg aacattccac
      541 tccgccggaa cggatcgaag aagtcctgac ggacattcgc ggctacattg aagtccaaca
      601 cgagctcgaa tccggtttta tcgttcgtct gcagaacgtc acgcaacacg gcttggaatt
      661 gctggtggtt tatatcgtaa agacgcccgt attcgaagag tttctggcgg tacaggagcg
      721 tatccacctg gagatcctcg gtattctgca tcgccatcag gtcaggttcg cgtcgacagc
      781 ccctgttgtc aacctctccg cttcgccgga tcgatagttc ttacaggatg ttgagggtcg
      841 ggtagtgatt cccgagaatc gtcgtcgcgt cttcgcccag ccattcttcc aggatcgtcg
      901 cgtaaacctg ccggaaatcg gttttgaact tcacgtctcc ctcgtcgaga tcacccaggt
      961 cgggtaggcc gttgatcaat ccttttcgac gcatattgcc tccggcgaga aagacatagt
     1021 tggcggcgcc gtggtcggtg ccgttgctgc cgttctgcgc cacccgacgg ccgaattccg
     1081 aaaagaccag gatgcgggtt tcgtagaatc gatcttgttg ctttaagtcg tggatcagcg
     1141 cgctcaggcc ctgatcaaag gttttcagca agcggttttg aatgttcttc tgtaaggcgt
     1201 gcgtgtcaaa ccccggaagt gaaacataaa aaaaccgtgt ctcaactccg gctccgatca
     1261 gcgaagcgat ctgacggagt tgtttgccga atgcatggtt cggatagttg cccgtcgtgt
     1321 gggtgctatt cgtatgtgcg gagagatagt ccgcgctctc gaccgtctgg cgaagggtac
     1381 gatacaggaa ggtgctttcc ccttgttccg gaagtgatgc ttgcgccagg ctgcgcaaca
     1441 gcggtcgctt cagcagttgt tgcaggcgtt tagaatcggg tgtggccatg ccctt
//
LOCUS       DAOTON010000773         1493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_67043_length_1493_cov_3.182197, whole genome shotgun sequence.
ACCESSION   DAOTON010000773 DAOTON010000000
VERSION     DAOTON010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67043_length_1493_cov_3.182197"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..>1493)
                     /locus_tag="P5292_12610"
     CDS             complement(148..>1493)
                     /locus_tag="P5292_12610"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS40014.1"
                     /translation="GLADLFLRQDQIERSRNMYRSALAYFKNSSNLNGEAVTLIGIGR
                     SFLKENHADSAKVYLESALQISSSNRYPAGIMNASHALAEAYERTGNAAKALTMQQQY
                     YTLRDSLFNLESTRQLAELQYQLDLTRKQSEIDLLSKERQIDRFLRIMLITGSALFLI
                     LSLLLLQRFRYKQQANRKLEKANQDLNHALDELRNTQEQLVQSEKMASLGQLTAGIAH
                     EIRNPLNFITNFAAVSRELADELNDAGTATERHELSADLKRNLEKIEEHGLRANSIVR
                     SMMLHARQGKGERQTVNLRDLTEENVHLAFHGMRASHPDFDVTLEKHLSIQLPALELV
                     QQDISRVLLNLLNNAFYAVRERSKKKEPGFRPTVRIRLDSEQGQAVIRIEDNGIGIAS
                     EKLDKIFEPFYTTKPPGEGTGLGLSISHDIVKGHGGSMRANSVGGWTVFEVRLPT"
BASE COUNT          313 a          376 c          445 g          359 t
ORIGIN      
        1 atagtgaata gtgaatagtg aatagtgaat agtgaatagt aaacagtaaa cagtaagcgg
       61 tactggcgct ttggagagaa gatcggaaac tcggaactcg gaactcggaa cacggaacac
      121 ggaacacttt actccctcgc cccttcttca agtaggcaaa cgcacctcaa agaccgtcca
      181 gccgccaacg ctgttggcgc gcatggagcc gccgtggcct tttacgatat cgtgactgat
      241 gcttaacccc agtccggtgc cttcacccgg aggtttggtc gtatagaatg gctcaaaaat
      301 tttatcaagc ttttcggagg cgataccgat gccgttgtct tcaatgcgaa tcacggcctg
      361 gccttgctca ctatcgaggc gaatgcgcac ggtgggacga aacccgggtt ctttcttttt
      421 cgaacgttca cggaccgcgt aaaaggcgtt gttcaggagg ttcagtaata cccggctgat
      481 gtcctgttgt accagttcca gtgccggaag ttgaatggag aggtgcttct cgagtgtgac
      541 atcgaagtcg ggatgcgagg cgcgcatgcc gtgaaaagcc aggtgtacgt tttcttccgt
      601 gagatcacgg aggttgacgg tctgtcgctc ccccttcccc tgccgcgcgt gcaacatcat
      661 gctccgtacg atcgaattgg cacgaaggcc gtgctcttcg atcttttcca gattgcgctt
      721 caggtcggcg gacagttcat ggcgttcggt agcggtaccg gcatcattca actcgtccgc
      781 cagctcccgg gaaaccgccg cgaagttggt gatgaagttg agcgggttcc ggatctcgtg
      841 cgcgataccg gcggtcagct gacccagcga agccatcttc tccgattgaa caagttgttc
      901 ctgggtgttc cgcagttcgt ccagcgcatg gttgaggtcc tggttcgctt tttcgagttt
      961 acggttggcc tgctgcttgt agcggaatcg ttggagcagc agaagactca ggatgagaaa
     1021 caaggccgaa cccgtgatca gcatgatgcg caggaaccgg tcgatctgcc gttccttgct
     1081 aagaaggtcg atctcggatt gctttcgggt caggtcgagt tggtattgca actccgcgag
     1141 ctggcgtgta ctttcaagat tgaagaggga gtcgcgcagc gtgtaatatt gttgctgcat
     1201 cgtcaacgct ttggcggcat ttccggtccg ttcatacgct tccgccaggg cgtgcgaggc
     1261 attcatgatt ccggcaggat agcggttgga ggaactgatc tggagcgcgg attccaggta
     1321 aaccttggcg ctgtccgcat ggttttcttt gagaaaggaa cggccgattc cgatcagggt
     1381 gaccgcttcc ccgttgaggt tggaagagtt cttgaaatat gccaaggcgg accggtacat
     1441 gttccgcgaa cgttcgatct gatcctgccg gaggaagagg tcggcaaggc ccg
//
LOCUS       DAOTON010000774         1492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_67107_length_1492_cov_4.016006, whole genome shotgun sequence.
ACCESSION   DAOTON010000774 DAOTON010000000
VERSION     DAOTON010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67107_length_1492_cov_4.016006"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="P5292_12615"
     CDS             complement(<1..1306)
                     /locus_tag="P5292_12615"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HRS40015.1"
                     /translation="MKYLTGFDRRQATLFPTCIDDLIPEDAEVRIIDLFVDALPLREL
                     GFFEKEPTEEGRPMYHPADLFKLYVYGYLNRIRTSRLLERECQRNIELLWLLKGLQPC
                     FRTIAGFRSENPKLFRNAFTHFVRQLNRKGLTGKTLVALDSSKFRAVNSKKNNYNQKK
                     IDRQLEYIDTRIQSYIDELNAGDLDEARQEAIGEKLKKQKAQRRKYKRIEKQLAETGQ
                     DQVSTTDPDARSMILHGSVIEVAYNVQTVADSANKLVLEYEVTNQNDRKALLPMSQKT
                     KSICATEAIAVLADKGYHNGEQLAACAQEDIYTIVAYPEVPRSHPVPTPEFYGDRFRY
                     NPKKDQYKCPQGHILKTTGHWYNKKYEKSVTRVKHYKTMACGSCEVRSLCTRNPNGRV
                     IERTEHAGAVERNNRRVRENTSVYSLRQQIIEHIFGTIKRQWG"
BASE COUNT          302 a          392 c          393 g          405 t
ORIGIN      
        1 agccccattg tcgtttgatg gtgccgaaga tgtgttcgat gatttgctgg cgaagtgaat
       61 agaccgaggt attttcccgt acacgccggt tgttgcgctc caccgcaccg gcgtgttcgg
      121 ttcgttcgat taccctcccg ttcgggttgc gtgtgcacag actccggact tcacatgagc
      181 cacaggccat agtcttgtag tgtttgaccc gggttaccga cttttcatac ttcttattat
      241 accaatggcc ggtggtcttc aggatgtgac cctgcggaca cttgtactga tcctttttag
      301 ggttgtagcg gaaccgatcc ccatagaact ccggtgtagg aacgggatga cttcggggaa
      361 cttccgggta agctacgatc gtgtaaatgt cctcttgtgc acacgcggca agctgttccc
      421 cgttgtggta acccttgtct gccagtacgg cgatggcctc cgtagcacag atggatttgg
      481 tcttttgaga catcggcagc aacgctttcc ggtcgttctg attggtgacc tcatactcca
      541 gcaccagctt gttggcagaa tccgccaccg tctgtacgtt gtaggcaact tcgatcaccg
      601 acccgtgcag gatcatgctg cgggcatcgg ggtcggtggt ggatacctgg tcctggcccg
      661 tttcggcaag ctgcttttca atgcgcttgt actttcgccg ttgagccttt tgctttttca
      721 gcttttcgcc gatggcctcc tgtcttgctt catccaggtc accggcattg agttcgtcga
      781 tatacgactg gatcctggtg tcgatgtact ccagttgccg gtcgatcttt ttctggttgt
      841 aattgttctt cttggagttg accgccctga acttgctgga gtccagcgcc acgagtgttt
      901 tcccggtcag gcccttacgg ttcaattgcc ggacgaagtg ggtaaaggca ttgcggaaga
      961 gcttcgggtt ttccgaccga aagccggcaa tggtccgaaa gcagggttgc aggcccttca
     1021 atagccaaag gagttcgatg ttccgctggc attcccgctc caacaggcgc gaggtacgta
     1081 tcctgttcag atagccatag acgtacaact tgaacagatc agccggatga tacatgggcc
     1141 gcccttcctc cgtcggttct ttttcgaaaa accccagttc ccggagcggc aaagcgtcca
     1201 cgaataaatc aatgatccgc acttcggcgt cttccggaat caaatcatcg atgcacgtgg
     1261 gaaataaagt ggcctgtctt cggtcgaatc ctgtgaggta tttcatgcaa agccggttat
     1321 tccctcaata taatcagccc cgcctgaaat caggtcaaaa ttctttaaac aataagggtg
     1381 ggtttttgca cagtctgacg gtttgcggct tggcgtagtg gcggattttt agcacaaaag
     1441 ttcaatcgaa gcactgcact tgaacctacc acaaaactgt catacgaagc ac
//
LOCUS       DAOTON010000775         1488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_67380_length_1488_cov_3.692254, whole genome shotgun sequence.
ACCESSION   DAOTON010000775 DAOTON010000000
VERSION     DAOTON010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67380_length_1488_cov_3.692254"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="P5292_12620"
     CDS             complement(<1..490)
                     /locus_tag="P5292_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRS40016.1"
                     /translation="MVVDIFIPCFIDQIFPETAWNMIKVLEKAGCTVNYNPEQTCCGQ
                     PAFNAGHWDECKAVGEKFIHDFRTDRYIVSPSASCVGMVKNYYPEMFHNSVLHNEYKG
                     IQRHIFELSDFLVNVLKVTNLGSKLEGVATYHDSCSGLREVGIRQEPRELLKNVAGLE
                     LRE"
     gene            complement(556..>1488)
                     /locus_tag="P5292_12625"
     CDS             complement(556..>1488)
                     /locus_tag="P5292_12625"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRS40017.1"
                     /translation="AGSIVFYNAYLPEIAEPQDQDRVSAKGFALGYIGSSLLLIFSLS
                     MVLFPDAYGGIEKGTAIRLAFLLVGVWWFGFAQITFYRLPDNVYGRPSSGHYLFNGYQ
                     ELRKVWNELRHTARLRTFLISFFFYNMGVQTVMYVATLFGDGELHLPSEILIAIILII
                     QFVAIGGAYLFSRLSKKFGNLKALAISIVTWIGICIGAWFCDKRFGVNEQNMFIGLAA
                     VVGLVMGGIQSLSRSTYSKLLPETIDHASYFSFYDLCDKFGTVLGTFAFGYINELTGS
                     MRNSIIVLAAFFVLGILLLLRVGPQRQLAAEPVG"
BASE COUNT          404 a          379 c          373 g          332 t
ORIGIN      
        1 tttcccgtaa ctccaatccg gctacgtttt taagcaattc ccgtggctcc tgccggatgc
       61 caacttcccg cagtccgctg caggaatcat ggtaggtagc gacgccttcg agtttggatc
      121 cgagattcgt cactttcaag acgttgacca aaaagtcact caattcaaaa atgtggcgtt
      181 gaatcccttt gtactcgttg tgaagcaccg agttatggaa catttcggga taataattct
      241 tgaccatgcc gacacaagaa gcggaaggcg aaacgatgta acggtcggta cggaagtcgt
      301 ggatgaattt ctccccaacg gccttgcatt catcccaatg cccggcgttg aaggctggtt
      361 gtccgcaaca agtttgctcc ggattgtagt tgaccgtaca gccggccttt tcaagcacct
      421 tgatcatgtt ccaggctgtc tccggaaaga tctgatcgat aaagcaggga atgaaaatat
      481 ctaccaccat aggcccgcca aagatacagg aaccctcggt ttcatcaacg ccttgcaagg
      541 gtccgctttc aacaattagc cgacaggttc tgcagccagt tgacgctgcg gcccgacgcg
      601 cagcaacagg agaatgccca atacgaagaa ggcggccagc acgatgatgc tgttgcgcat
      661 actgcccgtc agctcattga tatatccgaa agcgaatgtc cccagtacgg ttccgaattt
      721 gtcgcagagg tcgtagaaac tgaagtaaga ggcgtgatcg atcgtttcag gtaggagttt
      781 cgagtaggtt gatcgcgata acgattggat gccgcccatc accaggccaa ccacggctgc
      841 cagaccgatg aacatgttct gctcgttaac gccgaatcgc ttgtcgcaaa accaggcgcc
      901 aatgcagatg ccgatccagg taacaatcga gatggccagc gctttcaggt taccgaattt
      961 tttggaaagc cgcgaaaaaa gataggcgcc accaatcgcg acgaattgga tgatcagaat
     1021 aatggcgatc aaaatctcgg aaggcaaatg cagttctcca tccccgaaga gggtggcgac
     1081 atacatgacc gtctgtacgc ccatattata aaagaaaaaa gagatcagaa atgtacgtag
     1141 ccgggccgta tgtcgtaatt cgttccatac ttttcgtaat tcctgataac cgttgaacag
     1201 ataatgcccg ctgctgggtc ttccataaac gttgtcgggt aatcggtaaa aagtgatttg
     1261 ggcaaagccg aaccaccaga cacccaccag gagaaaagcc aaccggatag cggttccttt
     1321 ttcaatacct ccatatgcgt cgggaaacag taccatggaa agtgagaaga tgagcaagag
     1381 ggaactgccg atgtatccca gtgcaaagcc cttcgcactc acgcggtcct ggtcttgggg
     1441 ttccgcgatc tccggtaaat aagcgttgta aaaaacgata ctgcccgc
//
LOCUS       DAOTON010000776         1485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_67573_length_1485_cov_4.190210, whole genome shotgun sequence.
ACCESSION   DAOTON010000776 DAOTON010000000
VERSION     DAOTON010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67573_length_1485_cov_4.190210"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="P5292_12630"
     CDS             complement(<1..619)
                     /locus_tag="P5292_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRS40018.1"
                     /translation="MKRILPLLHFVWIALLFSACTPQRKIVYFQDGLPSALAQPGAYT
                     LRIYPGDILSINIFSINAEAFPYLAVPADRPTSDTRSAYEKGYIVAENGEVNLPLAGK
                     VNLNGMSLAEANRAITAKYKEYMEDPLVTVKKLNFKVTVLGEVNKPGTYPVPNERITL
                     AEALGYAGDLNTFGDRSAVKLLRSENNQMREYKIDLTKSSSLTPET"
     gene            complement(635..>1485)
                     /locus_tag="P5292_12635"
     CDS             complement(635..>1485)
                     /locus_tag="P5292_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRS40019.1"
                     /translation="EAFGSEAQKQKYLVPLAKGEIIGAFCLSEPEAGSDATSQHTTAI
                     DQGDHYLLNGTKNWITNGSSASVYLVMAQTDVAKGHKGINAFIVEKGMPGFTIGPKEN
                     KMGIRGSDTHSLMFADVKVPKENRIGEDGFGFKFAMKTLTGGRIGIAAQALGIASGAY
                     ELALQYSKERKAFGKTISQHQAIQFKLADMATNIEASRLLCLKAAWLKDQKQDYGLAS
                     SMAKLFASQTAMEVTTEAVQIHGGYGYVKEFHVERLMRDAKITQIYEGTSEVQRIVIS
                     REIIGK"
BASE COUNT          281 a          384 c          459 g          361 t
ORIGIN      
        1 aggtttcggg tgtgaggctc gagcttttcg tgagatcgat cttgtattcc cgcatctggt
       61 tgttctccga gcgcagcaat ttcaccgccg agcggtcgcc aaaggtgttg aggtcgcccg
      121 cataacccaa ggcctccgcc agggtgatgc gttcgttggg aacggggtaa gtgccgggct
      181 tgttgacttc gccgagcacc gtcactttga agttcagttt cttgacggtc accaaggggt
      241 cttccatgta ttctttgtac ttcgcggtga tggcgcgatt ggcttccgcg agggacatgc
      301 cgttcaggtt gaccttcccg gccagcggca ggttgacctc gccgttctcg gctacgatat
      361 agcctttttc ataagccgaa cgcgtatcgc tggtcggccg gtcggccggt acggcgagat
      421 acgggaaagc ttccgcgtta atggaaaaga tattgatcga gagaatgtct ccgggataga
      481 tccggagcgt ataggcgccg ggttgcgcca gagcggaagg aagtccatcc tggaagtaca
      541 cgatcttccg ctgcggcgta caggccgaaa aaagcagggc gatccacacg aagtggagca
      601 gcgggaggat tcttttcatc agcaggaacg aggattattt cccgatgatc tcacgggaaa
      661 tgacgatgcg ttgaacttcc gatgtgcctt cgtagatctg ggtgatcttg gcgtcgcgca
      721 tcagtcgttc aacgtggaat tccttcacgt aaccgtaacc accgtggatc tgtacggctt
      781 cggtcgtgac ttccatggcc gtttgggaag cgaagagttt ggccatggac gaagccagcc
      841 cgtagtcctg cttctggtcc ttcagccagg cggctttgag acaaagcagg cgggaagctt
      901 cgatgttggt ggccatatcg gccagtttaa actggatagc ctggtgctgt gagatggtct
      961 tgccgaaggc tttgcgctct ttcgaatatt gcagggccag ttcgtaagcg cccgaggcga
     1021 tgccgagcgc ctgcgcggcg ataccgatgc ggccaccggt gagggttttc atcgcgaact
     1081 tgaaaccgaa gccgtcttcg ccgatgcggt tttctttcgg gactttgaca tccgcgaaca
     1141 tgagcgagtg ggtgtcgctg ccgcggatac ccattttatt ttccttcggg ccgatcgtaa
     1201 aaccgggcat gcccttttcc acgatgaacg cgttgatgcc tttgtgacct tttgccacgt
     1261 cggtctgggc catgaccagg tacaccgaag cggaggagcc gttggtgatc cagttcttgg
     1321 tgccgttcaa caggtagtga tcaccctggt cgatcgcggt ggtgtgttgc gaagtcgcgt
     1381 ccgagccggc ttccggttcg gaaagacaaa aggcgccgat gatctcgccc ttggccagtg
     1441 gaaccaggta tttctgcttc tgggcttctg aaccgaatgc ttcga
//
LOCUS       DAOTON010000777         1485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_67598_length_1485_cov_2.518881, whole genome shotgun sequence.
ACCESSION   DAOTON010000777 DAOTON010000000
VERSION     DAOTON010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67598_length_1485_cov_2.518881"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..619
                     /locus_tag="P5292_12640"
     CDS             197..619
                     /locus_tag="P5292_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40020.1"
                     /translation="MKRATASLLLLSLFVVLFACNKDENPIVKEDTVQSTMKQGSWQV
                     TQIQDLGTYYSRQFKGRTFVFNSDGTATSTDSLGQTVRGSWYINAQGDMGVKISFGNA
                     NPENRLNRTFKVRDFSNHKFRLYTNGATASATYVTFER"
     gene            658..>1485
                     /locus_tag="P5292_12645"
     CDS             658..>1485
                     /locus_tag="P5292_12645"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HRS40021.1"
                     /translation="MRPELIHLTQMGAIRVCILFHLFLSGIRATAADDFLTALQRRDW
                     HAASRLANRADDAATDRSRLIDGFRLCLSGAYAEAQRTFHTLLQSDDSLTRALAIEAD
                     GQLLLLAGEWNALADLPATPGADTSGQAAERAFYRFFGRHERQTVRFDRDSSTVPMPL
                     RVSENPTLEAVINGRKARACWDTGAGICILSSSLASKLQVRTDGDVTAPVSSASAKQL
                     RMQAGIIDSIRIGAITWRNVPCGILDDRLLEFRVAGIRVAGFKAILGWPLMQSVDMTL
                     "
BASE COUNT          314 a          450 c          394 g          327 t
ORIGIN      
        1 ggaagctatc cgctcgacca ccgctcggtg cagcgcctgc aaccggaact ggcggcagga
       61 atttatctct accggctgac cagcgacggc gaaatcctga cgcacggtaa gggttattta
      121 cgctaaaagt cgctaccagc gcgggtttgg agggcttacg caagttgact acattcgtgc
      181 accaccatcc gataccatga aacgcgcaac agcatccctt ctgctccttt cccttttcgt
      241 cgtactcttc gcctgcaata aggacgaaaa tccgatcgta aaggaagaca cggtccaaag
      301 caccatgaag caaggcagtt ggcaggttac gcagatccag gatctcggca cctattactc
      361 ccggcagttc aaaggccgca ccttcgtgtt caattccgac ggcaccgcga cctctacgga
      421 ttcccttggc cagaccgttc gtggcagctg gtacatcaat gcgcagggcg acatgggcgt
      481 gaagatctcc tttggcaacg ccaatcccga gaaccgcctg aaccgtacgt tcaaggtccg
      541 cgatttttcc aatcacaagt ttcgattgta tacgaatggc gctacggcct cggccacgta
      601 cgtgaccttc gagcgctaac caatccttaa cgcttccgga aaaaccggga agctatcttg
      661 cgcccggaac tgattcacct gactcagatg ggagcaatac gcgtctgcat actttttcac
      721 ctttttcttt ccggcatcag ggcgactgcc gcagatgatt tcctgaccgc tttgcaacgg
      781 cgtgactggc atgcggcgtc ccggttggcc aatcgtgctg acgacgctgc taccgatcgc
      841 agccgcctca tcgacggatt ccggttatgt ctttccggcg cttacgctga agcccagcgc
      901 acttttcata ccctgctgca atcggacgac agcctgactc gcgcattggc gatagaagca
      961 gacggtcaat tgttattgct ggccggcgaa tggaatgccc tggctgatct tcccgcgact
     1021 ccgggcgctg ataccagcgg ccaggccgcc gaacgggcct tttaccgatt cttcggaagg
     1081 cacgagcgtc aaacggtccg ctttgatcgc gacagcagta cggttcctat gcccttgcgt
     1141 gtctcggaaa atcctacgct cgaagccgtc atcaacggaa gaaaagcccg ggcttgctgg
     1201 gacaccggag ccggcatctg cattctttcg tccagccttg cttctaaact gcaggtgaga
     1261 acggatggcg atgtcactgc cccggttagt tccgcttcag caaaacaact tcgtatgcaa
     1321 gcgggaatca tcgacagcat ccggatcggt gctattacct ggcgcaacgt gccctgcggt
     1381 atcctggacg accgactgct cgagttccgt gtcgcgggaa tccgcgttgc cggtttcaag
     1441 gcgatccttg ggtggccgtt gatgcaatcg gtagacatga ccctc
//
LOCUS       DAOTON010000778         1484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_67638_length_1484_cov_3.477957, whole genome shotgun sequence.
ACCESSION   DAOTON010000778 DAOTON010000000
VERSION     DAOTON010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67638_length_1484_cov_3.477957"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="P5292_12650"
     CDS             <1..300
                     /locus_tag="P5292_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40022.1"
                     /translation="FPISENTTWDGNDMNTLDEELYAYEDYHVSRSVNGMAFDSTLSV
                     LQRDDNNFVERVYGQEIYANGVGLIRRQRDDLGKRNGIVVKGTEFTMQVKDYGPR"
     gene            complement(368..>1484)
                     /locus_tag="P5292_12655"
     CDS             complement(368..>1484)
                     /locus_tag="P5292_12655"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRS40023.1"
                     /translation="VADKMKGFIGKEKRSIIPRDVVLYGFGRIGRLLARELISQAGKG
                     EQLRLRAVVTRTNTDEDITKRGDLLRNDSVHGSFPGTVIEDFKNKALIVNGHTVHMIA
                     ANDPASIDYTSYGIHDALVIDNTGVVRDREGLGKHLLAKGVSKVLLTAPGKGDVPNVV
                     YGVNENAFDHHKETVFSAASCTTNAIVPVLAVIEQNFGIERGHIETIHSYTNDQNLLD
                     NFHKKYRRGRSAALNMVITETGADKAVAKVLPQLAGKLTGNAVRVPTPDVSLAILNLT
                     LKKETSKDGINDVLRNAALSGDLVEQIQYSVSNELVSTDLVGNSCASIVDSPATIVSK
                     DNKSAVLYVWYDNEYGYSRQVIRYAKYLADVIRLRYY"
BASE COUNT          325 a          384 c          433 g          342 t
ORIGIN      
        1 ttccccattt cagaaaacac gacctgggac gggaacgaca tgaatacgct ggatgaagag
       61 ttgtacgcct acgaagacta tcacgtcagc cgttcggtga acggaatggc gttcgattca
      121 accctttccg tactccaacg cgacgacaat aacttcgtgg agcgtgttta tgggcaggag
      181 atctacgcga atggtgtcgg gttgatccgg cgtcagcgcg acgaccttgg taagcggaac
      241 ggaatcgttg taaaagggac ggaatttacc atgcaggtga aggattacgg acctcgatag
      301 acaggatgac cgataaaaaa agccgcccgg aaaatgtccg ggcggctttt tctttatcgg
      361 tgagtgatca atagtagcgc aggcggatca cgtcggccaa atacttggca taacggatca
      421 cctggcggct ataaccgtac tcgttatcat accagacgta caaaacggcg ctcttgttgt
      481 ccttggaaac gatggtagca ggggagtcaa caatcgaggc gcaggagttg ccgaccaaat
      541 ccgtcgagac gagctcgttg gagacggaat actggatttg ctcgaccagg tcgcccgaga
      601 gcgcggcatt gcgcagcaca tcgttgatgc catccttgct ggtttccttt ttcagggtca
      661 ggttaagaat tgcaagcgat acgtcaggag tagggacgcg caccgcgtta ccggtgagtt
      721 tgcccgccag ttgcggcaac accttggcga ctgccttgtc ggcacccgtt tcggtgatca
      781 ccatgttgag cgctgcggag cgtccgcggc ggtacttctt gtggaagttg tcgagcaggt
      841 tctggtcatt cgtataggag tggatcgttt cgatgtgtcc gcgctcgata ccgaagtttt
      901 gctcgatcac cgccagcacc ggaacgatgg cgttcgtcgt acaagaagct gccgagaaca
      961 cggtttcctt gtgatgatcg aaagcgtttt cgttcacgcc atacacgacg ttcggtacgt
     1021 cccctttgcc cggagcggtc agcaacactt tactgacacc ttttgcaagc aggtgcttgc
     1081 caagtccttc ccggtcacgc accacaccgg tgttgtcgat caccagggca tcgtggatgc
     1141 cgtagctggt ataatcgatg gaagcgggat cgttcgcggc gatcatgtgg acggtatggc
     1201 cgttcacgat cagcgctttg ttcttgaagt cttcgatgac cgttccgggg aatgaaccgt
     1261 gaacagaatc gttgcggagc agatcaccgc gtttggtgat atcttcatcc gtgttggttc
     1321 gggtcaccac ggcgcggagg cgtagctgtt ctcctttacc ggcttgcgag atgagctcgc
     1381 gggccagcaa gcggccgata cgtccgaaac cgtacaacac gacatcgcgc ggaatgatcg
     1441 agcgcttctc cttgccgatg aaccctttca tcttatcggc aacg
//
LOCUS       DAOTON010000779         1479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_67983_length_1479_cov_3.266854, whole genome shotgun sequence.
ACCESSION   DAOTON010000779 DAOTON010000000
VERSION     DAOTON010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67983_length_1479_cov_3.266854"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="P5292_12660"
     CDS             <1..877
                     /locus_tag="P5292_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS40024.1"
                     /translation="DRPDHLRRVLESITRNPESASSHLILRMDGAPAGASPDRLARMD
                     SVRQLLRERAWCKSVEIHESERNMGLAGSAMEGVNDAVARFGRVIVLEDDLECSPFFL
                     QFMNRALDVYADDHRVISISGYIYPIKATLPETFFLRGADCWGWATWKRGWDLFRSDG
                     NALLSELQSRQLSAAFDFDGSYPYTEMLTDQVNGKNDSWAIRWYASAFLLGKLSLYPG
                     RSLVQNLGIDGSGTHSGRSDRWQVPLAESAIAVERKESVEDPFVVERLQDYFRALNQP
                     DPFWKRVVRRLRRMI"
     gene            880..>1479
                     /locus_tag="P5292_12665"
     CDS             880..>1479
                     /locus_tag="P5292_12665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase, TIGR04325 family"
                     /protein_id="HRS40025.1"
                     /translation="MLRSRIRDLLPAFVMRWYRHRRWKQGLWPKSEWRGDYPSWASAE
                     ADSQGYENAMILERVTAATRAVQEGRAAFERDAVTFPERAFDPAVLAALQAMVAESGG
                     TIELIDFGGSLGSLYYQYRPLLNGVKELRWVVVEQPHFVERGKSITPPGELSFTHSLS
                     EIPGGEVNRVLLLGSVLSYLPNPWEFIREAQVYRFRFILI"
BASE COUNT          302 a          357 c          442 g          378 t
ORIGIN      
        1 tgatcgtccg gatcaccttc gacgcgtgtt ggagagtatc accagaaacc cggagtcggc
       61 ttcttcgcat ttgatattac ggatggatgg tgcaccggca ggagcctcgc cggatcgatt
      121 ggctcgtatg gattccgttc gccaactcct gcgggaacga gcgtggtgca agtcggtgga
      181 aattcacgaa tccgagcgaa acatgggctt ggcaggatcg gcgatggaag gggtaaatga
      241 tgccgtggcc cgatttggac gggtgattgt cctggaggac gacctggagt gctctccatt
      301 ttttttgcaa ttcatgaaca gggcactgga cgtatatgcg gatgatcatc gtgtaatcag
      361 tatcagcggt tacatctatc cgatcaaggc gactttaccg gagacgtttt ttctccgcgg
      421 cgccgattgt tggggctggg ctacctggaa aagaggctgg gatctgtttc gttcggatgg
      481 aaatgccttg ttgtccgaac tacagtcacg ccagctatct gccgctttcg actttgatgg
      541 ttcctatccc tacacggaaa tgctgaccga tcaggtaaat gggaagaacg attcctgggc
      601 gatccggtgg tatgcttcag cttttctctt gggaaagttg tcgctttacc ctggccgatc
      661 gctggtgcag aacctcggta tcgatggttc cggtactcac agtggtcgat ctgatcgctg
      721 gcaggtgcca ctggctgagt ctgccatagc ggttgaacgg aaggaatctg tggaagaccc
      781 gttcgtagta gaacggctgc aggattattt ccgggcgtta aatcagccgg atcccttttg
      841 gaaacgtgtt gttcgcagac ttcgacgaat gatctaagca tgttacgctc caggatccga
      901 gacctcttgc cggcattcgt catgcgctgg tatcgccacc ggcggtggaa gcaaggtctt
      961 tggcccaaat ccgaatggag gggcgactat ccttcatggg ccagtgccga agctgattca
     1021 caaggctacg agaacgccat gatcctggag cgggtcacag cagccactcg tgcagtgcag
     1081 gaaggtaggg cagcttttga acgggatgca gtcacatttc ctgagcgtgc ctttgatccg
     1141 gcggtgctgg ctgctttgca ggcgatggtt gctgaatccg gagggactat cgagctgatt
     1201 gattttggtg gttcactggg cagcttatac tatcaatatc ggcctttgct gaacggcgta
     1261 aaagaactcc gctgggtcgt ggtggaacaa ccgcattttg tggagcgcgg taagtccatt
     1321 acgcctccgg gtgagttgtc gttcacacat tccctttcgg agattccggg aggggaggtt
     1381 aaccgcgtat tattgctcgg aagcgttctg tcctatctgc caaacccctg ggaattcatc
     1441 cgggaagcac aagtctaccg ttttcgtttc atcctgatc
//
LOCUS       DAOTON010000780         1476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_68222_length_1476_cov_2.833216, whole genome shotgun sequence.
ACCESSION   DAOTON010000780 DAOTON010000000
VERSION     DAOTON010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68222_length_1476_cov_2.833216"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="P5292_12670"
     CDS             <1..747
                     /locus_tag="P5292_12670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40026.1"
                     /translation="PARLAPPTTSTAIGNLFLVASEDTVGPSGQMQVAVQLGQGSQPV
                     PGIYGVAFRLSFNPMVVDAAQSNFAFLPTSGMGTVGTNLLTFARPNWSAGYVDAVAVR
                     MDHTNASSDTTIALFDVVIIDNVSARTVCNFELTGVRAITQAGIYQQLTTYADSVDVN
                     SIPTGVSSPAALASIRLYPNPANTRLNWTGKETVSSITIADHLGRTVSYQEIGDNSRS
                     ISLETIPAGYYFVTLQTQSGATTRPLCIQR"
     gene            complement(1113..>1476)
                     /locus_tag="P5292_12675"
     CDS             complement(1113..>1476)
                     /locus_tag="P5292_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRS40027.1"
                     /translation="QHIIVCGHYGCGGVKAAMTHHNMGIINKWLRNIKDVYRFHRELV
                     DAVTDEEAKTDLMVELNVREQVMNLAKTSIIQKAWKSDNRPNLHGWVYGLKDGIIKPV
                     CEMPARTPLDDIYEYDDL"
BASE COUNT          329 a          445 c          323 g          379 t
ORIGIN      
        1 cctgcccgcc tcgcaccgcc gactacttca accgctatcg gcaacctttt cctggtagcg
       61 agtgaagaca ccgtcggtcc cagcggacaa atgcaggttg cggtacaact cggccaaggc
      121 tcgcagccgg ttccgggcat ctacggtgta gccttccggc tctccttcaa tccgatggtg
      181 gtggatgcgg cacaatccaa cttcgctttc ctgcccacct ccggtatggg aaccgtggga
      241 acgaacctcc tcacgttcgc tcgccctaat tggtcggccg gctacgtcga cgctgtggcc
      301 gtacgcatgg accacaccaa cgcttcgagc gataccacga tcgccttgtt cgatgtggtt
      361 atcatcgata acgtcagcgc ccgtactgtt tgcaacttcg aactgaccgg tgttcgcgcc
      421 atcacccagg ccggcattta tcagcaattg accacctatg ccgacagcgt ggacgtcaat
      481 tccatcccga ccggcgtttc atcgcctgcc gcgcttgctt ccatccgtct ctacccgaac
      541 cccgccaaca cgcgcctcaa ctggactggc aaggaaacgg tgagcagtat caccatcgcc
      601 gatcatttgg gccgcaccgt atcgtaccag gagatcggag acaacagccg cagcatttcc
      661 ctcgagacca ttccggcggg atattacttc gtcacccttc agacacaatc cggcgcgacc
      721 acacgccccc tctgcattca acgatagttt tttagttcca tgtttcctgt tggtaaaccg
      781 tccggcgttc cccgggcggt tttttttatg aagagtgggt gggagtgaat agtaaacagt
      841 gaatagtgaa tagcgaatag cgaatagcga atagcgaata gcgaaaaatt ttctaattac
      901 aaattactag ttaccaatta ccagttacaa atcccaatta caccttctgc caattttcgg
      961 tatgcctgaa gacaattaat tgtattcctt gaattcccgt cttgctttac accccataaa
     1021 taaaaaagag gccagtctgt ttgaccaacc tctttactat tttactattc gctattcgct
     1081 gtttactgtt cactgtttac tattcactaa cctcacaaat catcatactc ataaatatca
     1141 tccagcggcg tacgcgccgg catttcgcat acaggtttga taattccatc ctttaagccg
     1201 tacacccagc cgtgcaggtt ggggcgattg tcgctcttcc acgctttctg gatgatggag
     1261 gtcttcgcaa ggttcatgac ctgctcccgg acattcagct ccaccatcag gtcggttttc
     1321 gcttcttcat ccgttacagc atctaccagt tcccggtgga atcggtaaac atccttgatg
     1381 ttccgcaacc atttgttgat gatacccatg ttatggtgcg tcatcgccgc cttcacgcct
     1441 ccacaaccgt aatgtccgca cacgatgatg tgctgc
//
LOCUS       DAOTON010000781         1472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_68495_length_1472_cov_3.319689, whole genome shotgun sequence.
ACCESSION   DAOTON010000781 DAOTON010000000
VERSION     DAOTON010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68495_length_1472_cov_3.319689"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="P5292_12680"
     CDS             <1..618
                     /locus_tag="P5292_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40028.1"
                     /translation="STYYKVNVSGFGLNYSFPNIYHIGIDNSWFPLKISWDNTVLHDL
                     NMPFADVSPAPLAQIHFYYTFPFTWATENGSRIFPVDTEDLLCDSGCFFRDSLIIYPQ
                     AGYNIQTSSGFILLRIGPWTNGLHLGVPELDVDLKQSVIEDELFLQAGKPIRTVSIYQ
                     VTGALVVNRSRVDAPEFRTSLPAAGVWFVQVAGDDWVRTLKVIRQ"
     gene            complement(707..>1472)
                     /locus_tag="P5292_12685"
     CDS             complement(707..>1472)
                     /locus_tag="P5292_12685"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRS40029.1"
                     /translation="LNEPTLCYCTTNLGGNCDPGSYGFIDLVKITGTPLINDNSGCTG
                     TIGLYYTDYPDTGNYTATLLAGSSYTLTVRSSFNANESVWIDWDRNGTFDVTEWYDLS
                     RSATPNVADSVTITVPGWIIPGRTKMRVRNRLASAPNSAIDACTQMASGETEDYTLTL
                     EDPTRLHPTATAQLLAVFPNPARDVLVVQLPEQVFTGILSVTDLSGRMLLSNAIRREG
                     DGLYPLNLGDLPDGWYILHLQSSNEHRVSRFARIRE"
BASE COUNT          319 a          350 c          418 g          385 t
ORIGIN      
        1 agtacttact ataaggtaaa tgttagtgga ttcggcttaa attattcttt tccgaatatc
       61 tatcatatag gcattgataa ttcatggttt cctctcaaga taagttggga taatactgta
      121 ttgcatgatt tgaatatgcc ctttgccgat gtttctcctg cccctttggc gcaaatacat
      181 ttttattata catttccttt tacttgggca acggagaatg gttcacgcat ttttccggta
      241 gacactgaag acttactgtg tgattccggt tgtttcttta gggatagttt gattatttac
      301 cctcaggctg gctataacat ccaaacaagt tccggattta tcctgttgcg aatcggccca
      361 tggaccaatg gtctgcatct cggcgtgccg gagcttgatg ttgatctaaa gcaaagcgtg
      421 attgaagatg aattgttctt acaagccggc aagccgatcc gcacggtcag tatttaccag
      481 gtaaccggcg cgttggtggt taaccgaagc agggtggatg cgccggaatt cagaaccagc
      541 ctgcctgctg caggagtctg gtttgtacaa gttgccggag atgattgggt gcggacgctc
      601 aaggtgatac ggcagtaaac cttgccgata ccactttatc cggttgaagg agtagccgtt
      661 tccggccgcc tggtcggcaa acaagcgtac aggcgaaaga tttgaattac tcccggatcc
      721 tcgcgaaacg actcacccgg tgttcgtttg agctttgcaa atgcaggatg taccaaccat
      781 ccggcaaatc tcccaggttg agcggataca atccatcgcc ttcccttcgg atagcgttcg
      841 ataacagcat tcgtccggag agatcggtta cggaaaggat tccggtgaaa acttgttcgg
      901 gtagttgaac aaccagcaca tcccgggcag gattaggaaa tactgctaac aattgtgccg
      961 tggcggtcgg gtgcagacgc gtcgggtctt ccagtgtcag cgtgtaatct tcggtttcgc
     1021 cgctggccat ctgcgtacag gcgtcgatgg cggagttcgg tgcgctggcc aggcggttgc
     1081 gcacccgcat cttggtacgg ccggggatga tccagccggg cacggtgatg gtcaccgagt
     1141 cggctacgtt gggcgtggcg ctgcggctga ggtcgtacca ttccgttaca tcgaacgtcc
     1201 cgttgcggtc ccagtcgatc caaaccgatt cgttcgcgtt gaagctggat cgcacggtca
     1261 gggtgtaact ggagccggcc agcagggtgg ccgtgtagtt tccggtatcg ggataatcgg
     1321 tgtagtacaa gccgatggtt cccgtacagc cggaattgtc gttgatcagc ggcgttccgg
     1381 tgatcttcac caggtcgata aagccgtagg agccaggatc acagttgccg cccaggttgg
     1441 tggtacaata gcagagcgtg ggttcgttca gc
//
LOCUS       DAOTON010000782         1466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_68906_length_1466_cov_2.472006, whole genome shotgun sequence.
ACCESSION   DAOTON010000782 DAOTON010000000
VERSION     DAOTON010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68906_length_1466_cov_2.472006"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="P5292_12690"
     CDS             <1..386
                     /locus_tag="P5292_12690"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40030.1"
                     /translation="ITLPEIRKRVEPILQQLSEQEERQALEQLGAAIGNGHYAAGVAE
                     VWRAAAEARGRLLVVEKDYRIAARFGEDNYTILVDDAVETARHKIADAVDDIIEMVLQ
                     NNGEVVFVGNGRLEQQQHIALITRY"
     gene            398..1237
                     /locus_tag="P5292_12695"
     CDS             398..1237
                     /locus_tag="P5292_12695"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRS40031.1"
                     /translation="MKTILVPTDFSANAEHAIRYAVIFAKPFGAKVVLFHSYLLPVYA
                     TDLPLPVATDEALRLASQESLEKLATRMRSEFPGVNFETRLEAGYAEEAIRAAVEAMK
                     ADMVIMGTRGASGIREALIGTITASMLEVLDVPLIAVPAESPVKAPSRIVFATNYSEG
                     DFQHVLDVIEIAKGVKARVSLLHVSSGELEKEYEYEAIERFKERLKEDSHFANIDFNL
                     LENKDVYEGLSEFLVSSGADFVATSMRRRSFLQKLFTRSITKEMAYHTHIPLLAFKVG
                     DKR"
     gene            1248..>1466
                     /locus_tag="P5292_12700"
     CDS             1248..>1466
                     /locus_tag="P5292_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40032.1"
                     /translation="MVHTRFFEDLGRVLYVLAINDGQVQEREQEIVEQLIDEALLAHP
                     DFNEASHLKNAMLTKISFRNAVRNGMQTA"
BASE COUNT          346 a          396 c          420 g          304 t
ORIGIN      
        1 acatcacctt gccggaaatc cgcaaacggg tcgagccgat ccttcagcaa ttgagcgagc
       61 aggaagaacg gcaggcactg gaacaactgg gcgccgcgat cggtaacggt cactatgctg
      121 ccggagtcgc cgaagtatgg cgggccgcgg cggaagcacg gggcaggctt ctggtagtcg
      181 aaaaagacta tcgtatcgcg gcccgtttcg gggaggataa ttatactatc ttggtggatg
      241 atgccgtcga aacggcccga cataagatcg ccgacgcggt cgatgacatc atcgaaatgg
      301 tgcttcagaa caacggcgaa gtcgtatttg tcggcaacgg tcgtctggag cagcaacaac
      361 acatcgcact cattacccgc tattgacagc ccctcccatg aaaacgatcc tggttcccac
      421 cgatttctcc gccaacgcgg aacatgctat ccggtacgcg gtcatcttcg cgaaaccatt
      481 cggtgccaag gtggtgctct tccattccta cctcttaccc gtttacgcga ccgatctgcc
      541 actgccggtt gcaaccgatg aggccttgag attggcctcc caggagtcgt tggagaagct
      601 ggctacccgg atgcgttcag agtttccggg tgtgaacttc gaaacccggc tggaggccgg
      661 atacgcggaa gaggccatac gcgctgcagt ggaagccatg aaagcggata tggtcatcat
      721 gggcacccgg ggcgcctcgg gaatccgcga agccctcatt ggaacgatta ccgccagtat
      781 gctcgaagtg ttggacgttc cgctcatcgc ggtaccggcg gaatctccgg tcaaagcccc
      841 gtcccggatc gttttcgcta ccaattattc cgaaggcgat tttcagcatg tactggacgt
      901 gatcgagatc gcgaagggag tgaaagcccg ggtcagcctg ctgcatgtga gttcaggaga
      961 acttgaaaag gaatacgagt atgaggcaat tgaacgcttt aaggagcgat tgaaggaaga
     1021 cagccatttc gcgaacatcg acttcaatct cttggagaat aaggatgttt acgaaggctt
     1081 gagcgaattc ctggtctctt cgggtgccga ctttgtggcc acgtccatgc gccgccgttc
     1141 cttcctgcag aaattgttca ccaggagcat tacgaaagag atggcgtatc acacacacat
     1201 tccgctcctg gcattcaagg taggagacaa gcgttaaccg catcgccatg gtgcacacaa
     1261 ggttcttcga agacctcggt cgtgtattgt atgtcctggc catcaacgac ggtcaggtgc
     1321 aggagcggga gcaggagatc gtcgaacaat tgatcgacga agccctgttg gcgcatccgg
     1381 attttaatga ggcttcccac ctcaagaacg ccatgctgac caagatcagt ttccggaacg
     1441 cggtgcggaa cggcatgcaa accgcc
//
LOCUS       DAOTON010000783         1462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_69213_length_1462_cov_2.558635, whole genome shotgun sequence.
ACCESSION   DAOTON010000783 DAOTON010000000
VERSION     DAOTON010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69213_length_1462_cov_2.558635"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="P5292_12705"
     CDS             complement(<1..195)
                     /locus_tag="P5292_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013668374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRS40033.1"
                     /translation="MNIEELRNYCIRKPEVTEGFPFDSETLVFKVAGKMFLLVSLDES
                     PLRFNVKCDPELAIELREKYP"
     gene            305..721
                     /locus_tag="P5292_12710"
     CDS             305..721
                     /locus_tag="P5292_12710"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRS40034.1"
                     /translation="MKRSWLFSLLFLSGISVSAQDWIVTSNTDSSLVIGRDKRYDLLV
                     AKQRRLNITRQTVTGYRVQIYFGSNRPKASEVKIDFTSRYPDVPAYLSYQQPNYKVRV
                     GDFRSRFEAQQFLGKIEGQYPTMFIVPDEVRLPGLK"
     gene            complement(803..>1462)
                     /locus_tag="P5292_12715"
     CDS             complement(803..>1462)
                     /locus_tag="P5292_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HRS40035.1"
                     /translation="ITLDEIGRRLAIGNFKELNVIIKGDVDGSIEALTDAILKLSTQE
                     VQVNVIHKSVGQISESDVLLASASNAIIIGFQVRPSTSARKLAENEEIEIRLYSIIYD
                     AINELKQAMEGMLAPEYQEKIVGNIEIREIFKIPKVGTVAGCMVLDGKISRNTKVRVV
                     RDGIVQYSGELGSLKRFKDDVKDVSAGYECGLSVQNFNDIRVGDIIEGYEQVAVKRTL
                     A"
BASE COUNT          301 a          428 c          391 g          342 t
ORIGIN      
        1 agggtatttt tcccgcaact cgatcgccag ctccggatcg catttgacgt tgaagcgcaa
       61 aggcgattca tcaagggata cgagcaggaa catcttaccg gcaaccttga agacaagcgt
      121 ctcgctgtcg aacggaaagc cctcggtgac ttccggtttt cgaatgcaat agtttcgaag
      181 ctcttcgatg ttcatcgccg cgtattcgat cctgaattta gcgattcccc ggatgaacct
      241 gaccggaaaa tgaacccagc gtcgaaaaag cgttaatctc cggttcaaac cgtatttttg
      301 agccatgaaa aggtcgtggc tgttttcgtt gctcttcctg tcgggcatca gcgtgtcggc
      361 acaggactgg atcgtcactt ccaacaccga cagctcgctc gtcatcgggc gcgacaagcg
      421 ctacgatctg ttggtggcta agcaacgccg actcaacatc acccgccaga ccgtaaccgg
      481 ttaccgggta cagatctact tcggttccaa ccgcccgaag gcttcggaag tcaagatcga
      541 tttcacgtcc cgctacccgg acgtcccggc ctacctttcc tatcagcagc cgaactacaa
      601 ggtccgggtc ggtgatttcc gctcccgctt cgaagcgcag cagtttctcg gaaagatcga
      661 aggccagtat ccgacgatgt tcatcgtgcc ggacgaggtg cggctgccgg gattgaaata
      721 agctgcccct ttccttcatg caccaccata aaaaaacgcc ccggttaccg gggcgttttc
      781 tattgctgta tgttcagatg gatcaggcca gcgtacgctt gaccgccacc tgctcgtaac
      841 cttcgatgat gtcgcccacg cggatgtcgt tgaagttctg gacgctcaga ccgcattcgt
      901 agccggccga tacatccttc acatcgtcct tgaaacgctt gagcgagccg agttcgccgc
      961 tgtactgtac gataccgtcg cggaccaccc gcaccttggt gttgcgggag atcttgccgt
     1021 cgagcaccat acaaccggcc accgtaccga ctttcgggat cttgaagatc tcgcggatct
     1081 cgatgttgcc gacgattttc tcctggtact ccggcgccag catgccttcc atggcctgct
     1141 tgagttcgtt gatcgcgtcg tagatgatcg agtaaagacg gatctcgatc tcttcgttct
     1201 cggcgagttt gcgcgcgctg gtggaaggac ggacctggaa gccgatgatg atcgcgttcg
     1261 aagccgaggc gagcagtacg tcggattccg agatctgacc caccgacttg tggatcacgt
     1321 tcacctgtac ttcctgcgtg gaaagtttca ggatggcgtc ggtgagcgct tcgatcgaac
     1381 cgtccacgtc gcccttgatg atcacgttca gctccttgaa gttgccgatc gccaggcgac
     1441 gaccgatctc gtcgagggtg at
//
LOCUS       DAOTON010000784         1460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_69364_length_1460_cov_2.449822, whole genome shotgun sequence.
ACCESSION   DAOTON010000784 DAOTON010000000
VERSION     DAOTON010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69364_length_1460_cov_2.449822"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="P5292_12720"
     CDS             complement(<1..415)
                     /locus_tag="P5292_12720"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRS40036.1"
                     /translation="MMRIRLFLLFLLFGQVAFAQAPSATLNGQVVDKLSNQPIPFAPV
                     QISGTTIGTTTDEQGRFEFRGLTPGIYNIEVTSVGYDKAAVFEIDVSSTRVRPVRVEL
                     VARSTELEEVTVRPDPFVKLEEAPVSVYNVGEVEIK"
     gene            complement(464..1342)
                     /gene="dapA"
                     /locus_tag="P5292_12725"
     CDS             complement(464..1342)
                     /gene="dapA"
                     /locus_tag="P5292_12725"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRS40037.1"
                     /translation="MNLNKLKGTGVALVTPVHKDGSIDFKGFKKLLDRIIDGKVEYLV
                     PLGTTGESVTLTKDERRAVLDFVLEINDGRLPVVLGLGGNNTNEILECMEEMDFDGVS
                     AVLSVSPYYNRPSQRGIYQHYKMIANACPVPLILYNVPSRTGSNMDAETTLELAHDVR
                     NIIGIKEASGNLEKVMRILHGRPKDFLVISGDDLLTLPMLACGADGVISVIANAFPKD
                     WSEMVRAGLEGNFDRSRKLHYKLQEITRAIFVDGNPSGVKGLLSFQNVCAEHVRLPLV
                     SVGKSTINKFETILRG"
BASE COUNT          329 a          388 c          399 g          344 t
ORIGIN      
        1 gtttgatctc cacttcaccg acattataga cggaaaccgg ggcttcttcc agtttcacaa
       61 acggatcggg gcgaacagtc acttcttcca gctcggttga acgtgcgacg agttcgacgc
      121 ggacaggccg tacacgggtc gaacttacat cgatctcgaa aacagccgcc ttgtcgtagc
      181 cgaccgacgt gacttcgatg ttgtagattc ccggtgtcaa tccacggaat tcaaaccgac
      241 cctgttcgtc ggtggtcgtt ccgatcgtag taccggagat ctgtaccggt gcaaacggta
      301 tgggctggtt actgagcttg tcaacgacct ggccgttcaa cgtagccgaa ggggcctgcg
      361 cgaaggccac ttggccaaaa agcaggaaaa ggagaaaaag gcggatccgc atcatcgatt
      421 tcgcttgagg aaacaccttt cgctgcgaaa agttcagcgg aaatcagcca cggaggatgg
      481 tctcgaactt gttgatcgtg gatttaccga ccgataccaa tggaagccgg acatgctcgg
      541 cacaaacgtt ctgaaaactg agcaaccctt tgactccgga cggatttccg tcgacaaaaa
      601 tggcacgggt gatctcctgc agtttatagt gcagttttcg agatcggtca aaattacctt
      661 ccaagccggc ccggaccatt tcgctccaat ccttcggaaa tgcgttggcg atgaccgaga
      721 tgacgccatc ggctccgcag gcaagcatgg gaagggtgag cagatcgtcg ccgctgatca
      781 ccaggaagtc cttcggacga ccgtgcagga tgcgcatgac cttttccaga ttgccggaag
      841 cctctttgat tccgatgatg ttgcgtacat cgtgcgccag ttccagggtg gtctcggcat
      901 ccatgttcga gcccgtgcgg gaaggaacat tatagagtat gagcggaacc gggcaggcgt
      961 tggcgatcat cttgtaatgc tggtagatcc cccgctggga aggccggttg tagtacgggg
     1021 atacggacag caccgcggag acaccgtcga aatccatttc ttccatgcat tcgaggatct
     1081 cgttggtatt atttccaccc aatcccagca cgaccggcaa acggccgtcg ttgatctcca
     1141 gcacgaaatc cagcacagca cgacgttcgt ccttcgtgag ggtgaccgat tcacccgtgg
     1201 tgccaagcgg aaccaggtat tccacttttc cgtcgatgat cctgtcgagc agttttttga
     1261 accccttgaa atcaatgcta ccatccttat ggacgggtgt caccagggcg acaccggtcc
     1321 ctttgagttt attcaggttc atggttcagc gttttacttg gcgcaggtag tgctccgtct
     1381 cttcgatgac ggtttttata ccggtgtccg ggggcaactg caacatcatt tcatagcagg
     1441 tgatattgcg tttgtcatat
//
LOCUS       DAOTON010000785         1452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_69905_length_1452_cov_4.286328, whole genome shotgun sequence.
ACCESSION   DAOTON010000785 DAOTON010000000
VERSION     DAOTON010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69905_length_1452_cov_4.286328"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            683..>1452
                     /locus_tag="P5292_12730"
     CDS             683..>1452
                     /locus_tag="P5292_12730"
                     /inference="COORDINATES: protein motif:HMM:NF039475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6508 domain-containing protein"
                     /protein_id="HRS40038.1"
                     /translation="MLQQQFDRGYPPVTQTAWEKLLAFIPLLERSAPVGQWKEGSELI
                     AGVYVMPDVNYEPIIHEFIRTAYASGVITQVDWMNWPEQTELLQIGDETLLQQLGLNL
                     LRDLLTAILRQDRFVDGWLLAKLTDGTVLRILRALRYNVLHPLITDRETTRIYFADRL
                     QRDFPELFLRLIHLLDAFGISYTLLPAAEDIWCRDYMPVQVKSDKFVRFRYTTDQSAL
                     IPESIRSKTVSSDLRLDGGNIVKGPDRVALTDRVFDDN"
BASE COUNT          348 a          349 c          346 g          409 t
ORIGIN      
        1 ggcctttcgt gtgtcgccat tgcatgcagt tacataacga ctcattctgg gtaccgacat
       61 aatctgctca aaatcaacaa atctcatagt tgttaagtat aaaataaagg ttgctttctg
      121 cctgctttag cgttatattt gtcatcaaaa tatagttccc ggaagcccct gaagaaattc
      181 aagcttccgg gtttcgaatt ttatgcccct ggcacttttt tagtgattgc attttccatc
      241 ctattcatca gatgtaaaat tcggttttct ttgtttcaat tgtactttgc gtgatttttt
      301 cagcttttat taggttttta taaatgcgca tcatcctgca ggccaaaaaa ttcttgtcaa
      361 taagctgaat tcccttcgtc attccctctc cggcatcgat cgaactcccg ttggggaaat
      421 gaccgatgga agaatggagg caaacgccga atttatctgc gactttcctg taacaaccga
      481 ctactcatca ttcgatcgga atgaacagca gacatgcttg aaatcagcgc tgcgcctttt
      541 tatttattgc attattcgat aggaaacagc tattactaaa agaaatagta tatgcactaa
      601 acccggaaat tctggaactc ttctcagaat ttttggaact cgattcggtc ggttgctaat
      661 tcccgccata ccaatcaaga agatgctgca gcaacaattt gatcgtggct atccgcccgt
      721 aacacaaacc gcctgggaaa aattgctggc atttatcccg ctattggaac gatccgctcc
      781 cgttgggcag tggaaggaag ggagtgaatt gatcgcgggt gtttatgtga tgcccgatgt
      841 aaactatgag ccgatcatcc atgagttcat caggacggcc tatgcttcgg gtgtgatcac
      901 gcaggtcgac tggatgaact ggccggagca gacggaattg ttgcaaatcg gagatgaaac
      961 attgctgcag cagttggggc tgaacctgct gcgcgactta ttgacggcga tcctgcggca
     1021 agaccgcttc gtcgatggct ggctgttggc gaagctgacg gatgggaccg tgctgcgcat
     1081 ccttcgggct ttgcggtata atgtcctcca tcccctgatc acggatcggg agaccacccg
     1141 gatctatttc gctgaccggt tgcaacggga ttttcccgag ctattcctca ggttgatcca
     1201 tcttctggat gccttcggca tatcctatac gctacttccg gctgcggaag acatctggtg
     1261 ccgtgattac atgccggtac aggtgaagtc ggataagttc gtccggttcc ggtacaccac
     1321 ggatcagtct gctttgatcc cggaatccat taggtccaag acagtatcct ccgatctccg
     1381 cctggatgga gggaacatcg tcaaaggtcc cgaccgggtc gccctgaccg accgtgtgtt
     1441 tgatgataat ga
//
LOCUS       DAOTON010000786         1452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_69916_length_1452_cov_2.932713, whole genome shotgun sequence.
ACCESSION   DAOTON010000786 DAOTON010000000
VERSION     DAOTON010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69916_length_1452_cov_2.932713"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="P5292_12735"
     CDS             complement(<1..461)
                     /locus_tag="P5292_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRS40039.1"
                     /translation="MAKLKIRYQEDLLEAGVDEAGRGCLAGPVFAAAVILPPTYRHPL
                     LNDSKQVSEKDRYLLRDHIQEHALAWSVAEIGQAVIDDINILQATYRAMHRAIDGLTT
                     RPELLLIDGNRFLSYSGIPHQCIIDGDALYRSIAAASILAKTYRDDLMHRL"
     gene            complement(454..>1452)
                     /locus_tag="P5292_12740"
     CDS             complement(454..>1452)
                     /locus_tag="P5292_12740"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRS40040.1"
                     /translation="TPNVTPIVAGPAARHPILWYLWYEWSLPRVFRKWKPDLFVSSDG
                     YLSLRSQVPTLSVIHDINFEHYPGDLPFFNRAYYRHFFPRYAKAAARIATVSSFSRDD
                     ISRQYGISTDKIDVVYNGVNDGFVPLQESESRKTRDQLTGGKPYFLFIGSIHQRKNIV
                     NLLNAYEQFRTNGGPDVCLVFAGTKRWWTPEMETSLQNMRYRQDVHFTGRVAEADLFR
                     ITAAALALTYVSTFEGFGIPLLEAMRCGVPVLTANVTSMPEVAGDAALLVDPFSVEDI
                     SKGMQRLSVDAQLRQELSQRGLIRSRDFSWDFTARDLWNSIGRTMSTLENDSRRHG"
BASE COUNT          315 a          387 c          440 g          310 t
ORIGIN      
        1 tgcagccggt gcatgaggtc gtcccgatag gttttcgcga gaatcgaagc cgctgctatg
       61 gagcggtaca gcgcgtcgcc gtcgataatg cattggtggg gtatgccgga atacgacaga
      121 aaacggtttc catcgattag caggagttcc ggacgcgtcg taagcccgtc aatagcccgg
      181 tgcatggccc ggtaagtggc ttgcagaatg ttgatgtcgt cgatcaccgc ctgcccgatc
      241 tccgctaccg accatgccag tgcgtgctcc tggatatggt cccgcaacag gtaccggtcc
      301 ttctccgaaa cttgtttgga gtcgttcagt aaggggtgac gataggtggg cggcagtatg
      361 acggccgcgg cgaaaacggg accggccagg cagccacgac cggcttcgtc gacgccggct
      421 tccagcaagt cttcctggta gcggatcttc aacttagcca tggcgcctcg aatcgttttc
      481 cagagtggac attgtacgtc cgatgctgtt ccaaagatcc cgtgcggtaa aatcccagga
      541 gaagtcgcgg ctgcgaatca gtccgcgttg cgagagttct tgccggagct gagcatcgac
      601 actcaatcgt tgcatgcctt tggagatatc ctcaacggag aacgggtcga ccagcaaggc
      661 cgcgtcgccg gcgacttcgg gcatcgaagt cacattcgcg gtgagcacgg gtactccgca
      721 acgcatcgct tcaaggagcg gaatgccgaa tccttcgaag gtggatacat acgtcaatgc
      781 cagagccgcc gcggtgatcc ggaagagatc agcctccgct acgcgaccgg tgaagtgtac
      841 atcctgccgg taccgcatgt tttgcaggct tgtttccatt tccggtgtcc accaccgttt
      901 cgtgccggcg aataccaaac agacatccgg cccgccattc gtgcggaact gttcgtaggc
      961 gttgagcagg ttgacgatat tcttgcgttg atgaatggag ccgatgaaca gaaaatatgg
     1021 tttcccaccg gtcagttggt cccttgtttt cctgctttcc gactcctgca gcggtacaaa
     1081 tccgtcgttc acgccgttgt acacgacatc gatcttgtcg gttgatatcc cgtattgccg
     1141 gctgatatca tcgcgcgaga aggaagagac cgtggcgatg cgggcggctg ctttggcgta
     1201 acgggggaag aaatgtcgat agtaggcccg attgaaaaag ggcagatcgc cggggtagtg
     1261 ttcgaagttg atatcatgaa ttaccgaaag agtcggaacc tggctgcgaa gcgacaggta
     1321 accgtcggac gagacgaaca gatcaggctt ccactttcta aaaacccggg gcagggacca
     1381 ttcataccac agataccaga ggataggatg gcgggccgca ggacctgcaa cgatcggggt
     1441 cacgttgggt gt
//
LOCUS       DAOTON010000787         1451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_70002_length_1451_cov_2.838109, whole genome shotgun sequence.
ACCESSION   DAOTON010000787 DAOTON010000000
VERSION     DAOTON010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70002_length_1451_cov_2.838109"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="P5292_12745"
     CDS             complement(<1..237)
                     /locus_tag="P5292_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40041.1"
                     /translation="MFLKRLAFWFSLSLLLFSVQRSSASHSMGSDLTYQCLGGNTYQV
                     TLSFYRDCVGVPADGWADILIQSSCYPAFYITLDP"
     gene            complement(544..>1451)
                     /locus_tag="P5292_12750"
     CDS             complement(544..>1451)
                     /locus_tag="P5292_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRS40042.1"
                     /translation="IAQQSMAYASVITDAADNVTQQSPAYPPDGTNSLADQLKIVARL
                     IAGGLKTKIYLVSMGGFDTHSLQVDAADPTTGTHATLLGRVSEAISAFCADLDFLNIG
                     DRVLGFTFSEFGRRIKSNGSTGTDHGAAAPMFLFGPNVQPGMLGSNPIIPATVTSNDN
                     VAMQYDFRSVYATVLNQWLCVPAADLNQIMLQNFQLLPVVNGNACTVGINELNQKAGE
                     SLVSNYPNPFVERTRVKYSSMGGHTLVQVFNPEGQLVNTLVNTELPAGTYETDFENPG
                     LAAGIYHLRLQNESLQQVRNMMIVR"
BASE COUNT          389 a          335 c          389 g          338 t
ORIGIN      
        1 ggggtccagg gtgatgtaaa aagccggata acacgaagac tgaatgagaa tgtcagccca
       61 tccgtcggct ggaacaccga cacagtcgcg ataaaacgat aaggttacct gataggtatt
      121 gccgcccaga cattgatagg tcagatcgga ccccatcgaa tgactggcag atgatcgctg
      181 aaccgaaaag agtaacagcg aaagtgaaaa ccaaaacgca aggcgtttta ggaacatgga
      241 aattacttaa gtcctgtgtg cagaggaata agcaagctgc cttgaaaaat cgttgatgca
      301 cataggtaaa gaaactatga attggaagca aggtgcctaa aatgaacaaa agagctaatg
      361 gcaagtttta ataatcaatt cttggttgag gaggttttat ctcatacaac ttcaagaatg
      421 ataaaaaaac tcttcggcga attgaacgaa attgtcggta tagttgtacg atgaacagga
      481 atgattggaa taatacggta tcaattccaa tggttcgacc tgcaccaatt ttcttccttg
      541 agcctatcgc actatcatca tatttcgcac ctgttgcaaa gattcgttct ggagtcgcag
      601 gtgatagata cctgctgcga ggccggggtt ttcgaaatcg gtttcatacg taccggccgg
      661 aagttctgta ttgaccagag tattgaccaa ttgaccttcc ggattaaaga cctgaaccaa
      721 ggtgtgtccg cccattgaag aatacttcac gcgcgtacgt tccacaaagg gattcggata
      781 atttgacacg agcgactcgc cggctttctg gttgagttcg ttgattccga cggtacatgc
      841 atttccattt accaccggta gcaactggaa attctgcagc atgatctggt tcaggtccgc
      901 cgccggtacg cataaccact ggttgaggac cgtcgcataa accgatcgga agtcgtattg
      961 catcgcgaca ttatcgttcg aggtcacggt agccggaatg atcgggttgc tgcccagcat
     1021 accgggttgc acgttggggc caaacaagaa cataggtgct gcggcgccgt gatcggttcc
     1081 ggtactgccg ttcgacttga tccttctgcc gaattcggag aatgtaaagc ccaggacacg
     1141 atcaccgatg ttgagaaaat cgaggtcagc gcaaaaggcg gagatcgctt ccgacacccg
     1201 tccaagcagg gtcgcatgcg tacccgtcgt gggatcggct gcatcgactt gcaggctatg
     1261 cgtgtcgaaa ccgcccatgc tgaccaggta gatcttggtc ttgagtcccc cggcgatcag
     1321 tcgagcaacg atcttgagct ggtcggcgag tgaattggtg ccatccggcg gataagccgg
     1381 agattgctgc gtaacgttgt cggctgcatc ggtgatgaca ctggcgtaag ccatcgactg
     1441 ctgcgcgatc g
//
LOCUS       DAOTON010000788         1449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_70161_length_1449_cov_2.832855, whole genome shotgun sequence.
ACCESSION   DAOTON010000788 DAOTON010000000
VERSION     DAOTON010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70161_length_1449_cov_2.832855"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="P5292_12755"
     CDS             <1..253
                     /locus_tag="P5292_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRS40043.1"
                     /translation="KSISKIEEAILKALEMTPPELSADIYRTGIYLTGGGALLRGLDQ
                     RISRKTKLPVHVAEDPLRAVVRGTGIALKNIGRFKFLIP"
     gene            414..1247
                     /gene="mreC"
                     /locus_tag="P5292_12760"
     CDS             414..1247
                     /gene="mreC"
                     /locus_tag="P5292_12760"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRS40044.1"
                     /translation="MRNLFLFLWRYNFFIFFLLLEACCIYLIVQNSNYRRASFINSTN
                     RVAAEVNSGVSAVTEYINLRTTNEALARQNATLRGLFPDVYYIDSALRRTVSDTNYRQ
                     QYTFLTAKVVNNSVNRRSNYLTLDRGSLQGVKPEMGVICPDGVVGIVKDVSEHYCSVI
                     SFLHKDSRISARIRKNGYIGSMVWGGTDDAHGQLTDIAKHVKLAPGDTIVTSSFSAIF
                     PEGVMIGVIDRVNPETSDNFQDIDVKLSTPFASLSYVYIVGNLYKEEQRALEDSLSND
                     R"
     gene            1237..>1449
                     /locus_tag="P5292_12765"
     CDS             1237..>1449
                     /locus_tag="P5292_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRS40045.1"
                     /translation="MIVKLIQNLIRFLFLVAIQVVVLNHIQWSGYVNPYVYILFLLLL
                     PVETPKALQLVLGLVTGLMIDMFGNTS"
BASE COUNT          328 a          398 c          386 g          337 t
ORIGIN      
        1 caaatccatc tccaagatcg aggaggccat cctgaaggcg ttggagatga ctcctcccga
       61 gctttccgcc gacatctacc ggacgggtat ttacctgacc ggtggcgggg cactcctgcg
      121 aggccttgac cagcgcattt cccggaagac caaactgccg gtacacgtag ctgaagaccc
      181 cctgcgtgcg gttgtacgtg gaacgggtat cgcgctgaag aatatcggtc gcttcaagtt
      241 cctcatcccc tgatcggggt catgaacatc ttcccgtgtg aaatcagtcc tgtggtacct
      301 gcgggattcc agcctattat tcggatttta ccgagcctgc cgtcgcggga agccgtacct
      361 ttgtcttgcg gtgagtcccg ggccggtacg accgcaaaac cgggaatgaa cccatgagga
      421 acctcttcct ctttctctgg cgatacaatt ttttcatctt cttcctgttg ttggaagcat
      481 gctgcattta tctgatcgtg cagaacagca actatcggcg ggcaagtttc atcaattcca
      541 ccaatcgtgt ggcggcggaa gtgaattccg gggtgagcgc cgtgacggaa tacatcaacc
      601 tcaggacgac caacgaagcg ctggcgcgac aaaacgcgac cttgcgggga ctctttccgg
      661 atgtttacta catcgattcc gccctgcgga gaaccgtcag tgatacgaac taccgtcagc
      721 aatacacgtt tctgaccgcc aaagtcgtca acaactcggt gaaccgacgg agtaactact
      781 taacccttga tcgcggcagc cttcagggtg tgaagccgga aatgggcgtg atttgtccag
      841 acggcgttgt cggcatcgtc aaagacgtat cagagcatta ttgctcggtc atctccttcc
      901 tgcacaagga ttccaggatc agcgcgcgca tcaggaagaa cggctatatc gggtcgatgg
      961 tctggggcgg cacggacgac gcgcacgggc aattgaccga catcgcaaag cacgtaaaac
     1021 tcgctccggg cgatacgatc gtcaccagtt ccttctctgc catcttcccg gaaggcgtga
     1081 tgatcggcgt gatcgaccgg gtgaatccgg agacatcgga taattttcag gacattgacg
     1141 taaagctgtc gacacccttt gccagtctca gttatgtcta catcgtaggc aacctgtaca
     1201 aagaagaaca acgagcgttg gaggactcgc tatcgaatga tcgttaagct gatccagaac
     1261 ctcatccggt tcctcttcct ggtcgccatc caggtggtcg tgctgaatca catacagtgg
     1321 agcgggtacg tgaacccgta tgtatatata ttattccttt tgttgttgcc ggtcgagact
     1381 ccaaaggcct tgcaactcgt actcgggctt gtaaccggac tgatgatcga catgttcggc
     1441 aataccagt
//
LOCUS       DAOTON010000789         1446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_70394_length_1446_cov_3.549964, whole genome shotgun sequence.
ACCESSION   DAOTON010000789 DAOTON010000000
VERSION     DAOTON010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70394_length_1446_cov_3.549964"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="P5292_12770"
     CDS             complement(<1..504)
                     /locus_tag="P5292_12770"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRS40046.1"
                     /translation="MNLRIRFLILFLAACTITSAAGQKKLRSLNHNAFRPGEVLKFRI
                     HYGFMDAGEATLEVKPELKNFGNRDCYHVVGTGRSVGAFDWFFKVRDRYESVIDKDAM
                     LPWLFIRRVNEGGYIINQNVSFNHYSDSAKSEKATISIPENTQDLVSAFYYARTIDFS
                     NAKEGDIF"
     gene            complement(1111..1446)
                     /gene="scpB"
                     /locus_tag="P5292_12775"
                     /pseudo
     CDS             complement(1111..1446)
                     /gene="scpB"
                     /locus_tag="P5292_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
BASE COUNT          263 a          354 c          471 g          358 t
ORIGIN      
        1 aaagatgtcg ccttctttgg cgttgctgaa gtcgatggtg cgggcgtagt agaaggcgga
       61 gaccaggtcc tgggtattct cggggatcga gatggtggct ttctcgcttt tcgcggaatc
      121 gctgtagtgg ttgaagctga cgttctggtt gatgatgtaa ccgccttcgt tgacgcggcg
      181 gatgaacagc cagggcagca tggcgtcctt gtcgatgacg gactcgtagc ggtcgcggac
      241 tttgaagaac cagtcgaagg cgccgacgga gcggccggta cccacgacgt ggtagcagtc
      301 gcggttaccg aagttcttga gttcgggttt tacttcgagg gtggcttcgc cggcatccat
      361 gaatccgtag tggatgcgga atttgagcac ttcgccgggg cggaaggcgt tgtggttcag
      421 ggagcggagt ttcttctggc cggcggcgct ggtgatggtg caagcggcga ggaagaggat
      481 caggaatcgg atgcgaagat tcatggcgcc ttgacttggc aaagattgtt ccaaggagta
      541 aaatgctggt ttacagcctt attctttagc aaagataggg tatggaacaa aacaatcgaa
      601 tggattaacg gaggattaac tatttcgatt gtggcaactg ccaggtaaaa aaaccacgga
      661 ggactcagag gtattcacag aggacaccga gggcggaagg cagttggcag tatgcagcta
      721 gcaagaccct cgcacctcgt cgctcgtccc acgtccctga tcgaacgatc ggaattactc
      781 gctttcgttc cggaagggcg ctttttcgtc agccgcttcg gattcagggg cgccgggctg
      841 gttttctaaa aaaatctgct tgcggtcggg cagttcgagg tcttcggctt cgccggcggc
      901 gggcgggact gcttcgttca gctcctccgc ttcttcgctc agcataacgg gcgcactata
      961 gtcttcggag cccatgagcg gcgcttcgat ggtgtcgcct tcttcgttga gcgtttccga
     1021 gagtaccggt gtttcggaac cggcttccga catggccgtc acttcgctgt cctgcgtgtt
     1081 ggcttccgtt tccggctcgg cttccatgag ttcggacggc tgaccgatct cgttctgttc
     1141 gaggtggagg tctttgagct gcggcaggtc tttcaccgac ttgatgccga agtaatccat
     1201 gaagctgtcg ctcgtggagt acatgatcgg tcggccgggg ccttcgctct tgccggagat
     1261 ggcaacgagt tctttctcca gcagcttctg gatggcgtag tcgcaactga cgccgcggat
     1321 gtgttcgatc tccgacttgg tgatcggctg cttgtaggcg atgatggcca gggtctccat
     1381 ctgggcgacg gagagtttct tcttggcctt gtgctggatg agcgcgctga ccgtcgccca
     1441 gatgga
//
LOCUS       DAOTON010000790         1446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_70396_length_1446_cov_3.398275, whole genome shotgun sequence.
ACCESSION   DAOTON010000790 DAOTON010000000
VERSION     DAOTON010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70396_length_1446_cov_3.398275"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="P5292_12780"
     CDS             complement(<1..789)
                     /locus_tag="P5292_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS40047.1"
                     /translation="MEPTPSRILVVDDEPDLELLIRQRFRHKIRNGEFLFEFAGNGQE
                     ALDKIGSDSRYEMILTDINMPVMDGLTFLNHLKERSRSCKAVVVSAYGDLENIRTAMN
                     RGSFDFITKPIDFTDLETTILKTLDEVTRLREGERARSQLTQTIQEKERAEQSEKFKQ
                     QFLANMSHEIRTPMNSIIGLTSLLLKTELIDQQRRYLQVIRKSSENLLVIINDILDLS
                     KIEAGKMTFESIDFSLADCLDTVYHTLLFKSEEKGLEFTMDIEPE"
     gene            complement(827..1210)
                     /locus_tag="P5292_12785"
     CDS             complement(827..1210)
                     /locus_tag="P5292_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015118100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS40048.1"
                     /translation="MNSMIKMLVVDDEPDVEALFTQRFRKEVRDQQVRIHFAFSGEQA
                     LNYLRTLDPFDLVLVLSDINMPGMTGLELLGIIRKEFPTLKVMMVTAYGDQSNYDKAR
                     ELGANDFVTKPVDFADLKARILAMA"
     gene            complement(1228..>1446)
                     /locus_tag="P5292_12790"
     CDS             complement(1228..>1446)
                     /locus_tag="P5292_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019990273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS40049.1"
                     /translation="LLISIRDNGTGIPGEIRDKIFNPFFTTKPTGQGTGLGLSISYDI
                     IVKGHRGDLQVESQPGSFTEFRIYLPVS"
BASE COUNT          297 a          368 c          414 g          367 t
ORIGIN      
        1 ttcgggctcg atatccatgg tgaattccaa acccttctct tccgacttga acaggagcgt
       61 atggtaaacc gtgtcgaggc agtctgcgag ggagaaatcg atggactcga aggtcatctt
      121 tccggcttcg atcttggaaa ggtcgaggat gtcgttgatg atgaccaaga gattctccga
      181 tgatttccgg atgacttgca ggtaacggcg ttgctggtcg atcagctcgg ttttcaggag
      241 caggctggtc agaccgatga tggaattcat cggggtccgg atctcgtgac tcatattcgc
      301 caggaactgc tgcttgaatt tttcgctttg ttccgcgcgt tctttttcct gaatggtttg
      361 ggtaagttgc gaacgggctc tttcgccttc ccgcaggcgg gtcacctcat cgagggtttt
      421 caggatcgtg gtctcgagat ccgtgaagtc gatcggtttg gtgatgaagt cgaacgatcc
      481 gcggttcatc gcggtacgga tgttttccag atcgccataa gcgctgacca ccacggcctt
      541 gcacgaccgg ctgcgctctt tcagatgatt aagaaacgta aggccgtcca tgaccggcat
      601 gttgatgtcg gtgaggatca tttcgtagcg actgtcgctg ccgatcttat cgagcgcctc
      661 ctgcccattc ccggcgaatt cgaacaggaa ctccccgttc cggatcttgt gccggaagcg
      721 ctgacggatc agcagctcca ggtccggttc gtcgtcaaca acgagaatgc gggagggtgt
      781 gggctccatg gttcaggcta aatttaagaa aaacatccgg cttgcctcag gccatggcca
      841 ggattcgcgc ttttaagtcg gcaaaatcga cgggttttgt cacgaaatcg ttcgcgccga
      901 gctcccgggc cttgtcataa ttgctttgat cgccataggc tgtgaccatc atgactttca
      961 gggttgggaa ttccttgcgg atgattccca gcaactccag gccggtcatg cccggcatgt
     1021 tgatatcgct cagtaccagc accaggtcga acggatcgag cgtacggagg tagttcaaag
     1081 cctgttcccc gctgaaggcg aaatggatcc ggacctgctg gtcgcggact tctttccgga
     1141 aacgttgggt gaagagggct tccacatccg gttcgtcatc gacgaccagc atcttgatca
     1201 tggaattcat gtgattggcg cgtttattta ggagacaggt agatagatcc tgaactccgt
     1261 gaaagaaccc ggttgcgatt ccacctggag atcgccccgg tgtcccttga caatgatatc
     1321 gtagctgatc gacaatccca aaccggttcc ttgcccggtc ggcttggtgg tgaagaaggg
     1381 gttgaagatc ttatcacgga tctcacccgg gataccggtt ccgttgtcgc gtatactgat
     1441 cagcag
//
LOCUS       DAOTON010000791         1438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_70974_length_1438_cov_3.360810, whole genome shotgun sequence.
ACCESSION   DAOTON010000791 DAOTON010000000
VERSION     DAOTON010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70974_length_1438_cov_3.360810"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="P5292_12795"
     CDS             <1..455
                     /locus_tag="P5292_12795"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRS40050.1"
                     /translation="VLNTVLGGYFGSRLMSNIREDKGYTYGIGSALVSMKEAGYFFIS
                     TEVGADVTEAALKEIYFEIDRLCNDPVPESELAMVRNFLLGNFLKGIDGPFALLDRSK
                     TLVVNGLDYDYYTHYIKVLQSVTPAELLELARRVWKPDSFTELVVGKR"
     gene            546..>1438
                     /locus_tag="P5292_12800"
     CDS             546..>1438
                     /locus_tag="P5292_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40051.1"
                     /translation="MRLIRLLLLFFFGLPLAGVAQQFHVRCLSVGNTGDVTVTWDRNG
                     LSPADFRCYYLYHSTSAAGPFSPIDSIFIYGDTSEVHATALANTNPAWYYLAFKSASG
                     APDILSDTASAIFLNVINPNSGFAVMNWNTLRDPLIATNSVYYRLYREFPTGIFTLID
                     SVDASLGVRPMTYNDLIAICNDTVRYRIEVADQGGCTSRSNRDGENFRDLLPPEIPQF
                     DSVSVDGAGNIVMGWSQNTSPDTRYYEIQINTGSSSAPVWTPLDTAFGIGTTYLNSGI
                     DGTGAPRDFKIVAVDSCGNQSA"
BASE COUNT          306 a          382 c          373 g          377 t
ORIGIN      
        1 cggtactcaa cacggtcttg ggtggctatt tcggatcgcg gctcatgtca aacattcgtg
       61 aagataaggg ctatacctac gggatcggtt ccgcgctggt ttccatgaaa gaagcaggct
      121 acttctttat ctctacggaa gtcggggccg atgtgaccga agcggctttg aaagagattt
      181 atttcgagat cgaccggctt tgcaacgatc ctgttcctga atcggaactg gccatggtac
      241 gcaacttctt gctgggtaat ttcttgaaag gaatcgacgg accgttcgca ctactggatc
      301 gaagtaagac cctggtcgtt aacggactcg actacgacta ctacacgcat tacatcaagg
      361 tgctgcagtc ggtaactccg gcggaattgc tggagcttgc aagacgcgtt tggaagccgg
      421 attcgttcac cgaattggtg gttggtaaac gctgaaaagg agcgttttta ccacatcccc
      481 tcggccattc gccccctttc cgttgacccg cgcgaccgga aagcgtacct ttagggcttg
      541 tcctcatgag gttgatccgg ctgttactgt tgttcttttt cgggcttcca ctagccgggg
      601 tcgcacagca atttcatgtg cgttgcctca gtgtcggtaa cacgggcgac gtaaccgtaa
      661 cctgggacag gaacggactt tcaccggcgg attttcgttg ttattatctg taccactcca
      721 cgtctgctgc aggtcctttt agcccgatcg acagtatttt catttatggg gacaccagcg
      781 aagtgcatgc cactgcattg gcgaacacca atcccgcctg gtattatctc gcattcaaat
      841 ccgcttcggg tgctccggat attctttccg ataccgcttc ggcgattttt ctgaatgtga
      901 tcaaccctaa ttccggtttc gcggtcatga actggaatac gcttcgagat ccccttattg
      961 cgaccaattc ggtttactac agactctacc gggaatttcc tacaggtatt ttcaccctta
     1021 ttgattcggt ggatgccagc ctgggcgttc gcccgatgac ctataacgac ctcatcgcca
     1081 tctgcaacga caccgtgcgt taccggattg aagtggcgga tcaaggcggt tgtacttcca
     1141 gatccaaccg ggatggagag aatttccgcg acttgttgcc gccggagata ccacagttcg
     1201 attccgtgtc ggtcgatggt gccggtaaca tcgtaatggg ttggtcgcag aatacttcac
     1261 cggatacgcg ttattatgag attcaaatta ataccggatc ctcatccgct ccggtttgga
     1321 cacccctgga cacggccttc ggcatcggaa cgacgtatct gaattccggg atcgatggca
     1381 ccggcgcgcc acgcgacttt aaaatcgtag ccgtcgacag ttgcggcaat cagtcggc
//
LOCUS       DAOTON010000792         1438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_70996_length_1438_cov_2.534346, whole genome shotgun sequence.
ACCESSION   DAOTON010000792 DAOTON010000000
VERSION     DAOTON010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70996_length_1438_cov_2.534346"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1005)
                     /locus_tag="P5292_12805"
     CDS             complement(175..1005)
                     /locus_tag="P5292_12805"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40052.1"
                     /translation="MKKYFTLAVLPIAAFIVFTAETASDNGKAGYTGSPNELLCDDCH
                     DAYTINTGGGSVVLSSANMNNWEYVPGQTYNMTLTVSRSGNPLFGMGLEALDASNANA
                     GTLTITDAASTQIKTKLVSSVSRRNVVHELDGGATPNSKDFDFSWTAPATDIGPVTFY
                     FAGVASNADGDEAIGDYVYNGVQVVTPANTTGIGQASARNSFCFPNPASSQLSVRVQG
                     TTIVRLIDTEGRTVRSIRQEEAVSQVITLDQLDVLPRGLYFLELQQAGTRQLEKVILQ
                     "
     gene            1201..>1438
                     /locus_tag="P5292_12810"
     CDS             1201..>1438
                     /locus_tag="P5292_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS40053.1"
                     /translation="MKVLIVEDEQELLKSMVDYLRDIGMECRQAHNLQEAISQIESAS
                     FDCIVLDIGLPDGSGLKVIEEMQKKEHQTSILIVS"
BASE COUNT          335 a          355 c          403 g          345 t
ORIGIN      
        1 cctgtacctg ggtctgaaaa ctgacctcag gagtcgcggg aacttccgga tcggtccggc
       61 aagcgctgct ggtgatcagc agaccaaagg cggcaagaag caatttgtta tgcattgggg
      121 atttggtttg acgacgatct catgaaaaag ccccggcgat tccggggctt tcatttattg
      181 tagtattact ttttcaagct ggcgcgtgcc ggcttgttga agttcgagga aatacaaacc
      241 gcgcggcagg acatccagtt ggtcgagggt gatcacctgg gatacggcct cttcctgacg
      301 aatgctgcgc accgtgcgtc cttcggtatc gatcaaccga acgattgtgg taccctggac
      361 cctaaccgag agttggctgg aagccgggtt cgggaaacag aagctgttcc ttgcacttgc
      421 ttgtccgatg ccggtcgtgt ttgcaggagt gacgacctgg acaccgttgt acacatagtc
      481 gccgatcgct tcgtcaccgt cggcattgga ggctacgccg gcaaagtaga acgtgacagg
      541 tccgatatcc gttgcgggag cggtccagct gaaatcaaaa tccttgctgt tgggagttgc
      601 gcctccatcc agctcatgta ccacattgcg ccgcgatacg gagctcacca atttggtctt
      661 gatctgagtc gatgccgcat cggtaatcgt cagggttccg gcgttggcat tggatgcatc
      721 cagcgcttcg aggcccattc cgaacaaggg gttgccgctt cgggataccg tcaacgtcat
      781 gttgtacgtc tgacccggta catattccca gttgttcatg ttggcactgc tgaggactac
      841 ggagccgcca ccggtgttaa tggtataggc atcgtgacaa tcgtcgcaca acaattcgtt
      901 gggggagccg gtatagccgg cttttccgtt gtcgctggcc gtttcagcgg taaagacgat
      961 gaaagccgct atgggcagca ccgccagggt aaagtatttt ttcatgaaat gcgcaggtag
     1021 gatacaaatc ctcgttaggg ccgcagcaaa gaggaacgat ttccaaatgt aaacctgaga
     1081 aagggaatct gaattgactc tgaataatcg attgcctggt caattatcca tgatttatcc
     1141 cgtttccctt catacttttg acaaaactgc ggtaaacgca gaaaaatatc ggccaagcac
     1201 atgaaagtat tgatcgtcga agatgagcag gagttgttaa agtccatggt agattatctg
     1261 cgtgatatcg gcatggaatg ccggcaggcg cataatctgc aggaggcgat ctcccagatc
     1321 gagtctgcgt ccttcgattg tatcgtcctg gacatcggct tacccgacgg aagcggcctc
     1381 aaggtcatcg aggagatgca gaaaaaggaa caccagacca gtatcctcat cgtttccg
//
LOCUS       DAOTON010000793         1434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_71307_length_1434_cov_2.467730, whole genome shotgun sequence.
ACCESSION   DAOTON010000793 DAOTON010000000
VERSION     DAOTON010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71307_length_1434_cov_2.467730"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="P5292_12815"
     CDS             <1..850
                     /locus_tag="P5292_12815"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40054.1"
                     /translation="GQYSVTPSLDFERETSFDVPSGMNHRPPVLPGITVNTGYNPFKE
                     SGDRIRTSGWEKMYEGLDQVAVAQASLIPDPAELFPDAAATSETASDEMAPATGEIIS
                     IDSRYLAFPMHGGLCLVDQQAAHERILFEELLNRPEEAIQGSQQELFPVHLQFRTADA
                     ELLAQHLADLRRFGFMLESFGGSAFVLQATPVGVERGTEQSVLEHILENLKHFHPDPT
                     EPVREQLARALARSLAIPAGRELTPVKRQELIEALFRCSNPGMGIDGKPCIVRISQEE
                     LRGRFR"
     gene            913..>1434
                     /locus_tag="P5292_12820"
     CDS             913..>1434
                     /locus_tag="P5292_12820"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRS40055.1"
                     /translation="MPEAVKNILIINGLLFLATIVLGNKGIDLTSILGLHYWGGSDFR
                     FFQFITYQFMHGGFAHIFFNMFAVWMFGTAIEQVWGSRRFLTYYLLTGVGAALCHYGL
                     FYFELQPLQALFETYQQSPTVENLETLLHSPLLHTYWSAEVESSIRSFVEEFNVQYSA
                     DPEKAMIYSVGYVK"
BASE COUNT          317 a          399 c          368 g          350 t
ORIGIN      
        1 gggtcaatac agcgtaacgc cttcgctgga ttttgagcgg gagacatcct tcgatgttcc
       61 gagcggcatg aaccaccgtc cgccggtcct tcccgggatc accgtcaata ccggttacaa
      121 ccccttcaaa gaatccggtg accgtatccg gacatccggc tgggagaaaa tgtacgaagg
      181 actggatcag gttgccgttg ctcaggcttc tttgatcccg gatcctgccg aattgtttcc
      241 ggacgcggcc gcgacaagcg aaacagcatc cgatgaaatg gctcccgcga ccggcgagat
      301 catcagcatc gattcacgtt acctggcttt cccgatgcat ggcggacttt gcctggtcga
      361 ccagcaggct gcccatgaac ggatcctttt cgaggaactc ctcaaccggc ctgaggaggc
      421 cattcaggga agccagcagg aactctttcc ggttcattta cagttccgga ccgcggatgc
      481 cgagttacta gcccagcacc tggctgacct tcgccggttc ggttttatgt tggaatcatt
      541 cggcggtagc gcttttgtcc tgcaggcaac acctgttggc gtggaacgcg gtaccgaaca
      601 atccgtactg gagcacatcc tggagaacct gaagcacttt catccggatc ctaccgaacc
      661 ggtacgagaa caactggcca gggcccttgc acgttcactg gcaattccag ctggccgtga
      721 gttaacaccc gtaaagcgac aggaacttat tgaagcattg ttccggtgtt cgaatccggg
      781 tatgggcatc gatgggaagc cctgcatcgt gagaatttcc caggaggaac ttcgtggacg
      841 tttccgctga actgaaaaaa tcgaccgagc actgatatga acgactacca acgtccttcc
      901 ggattcagcc tcatgccgga ggcggtcaag aatatcctca tcatcaatgg ccttctgttt
      961 ctcgctacga tcgtactcgg taacaaggga atcgatctga ccagtattct cggtcttcat
     1021 tactggggtg gttccgattt tcgttttttc caatttatca cctaccagtt catgcacggt
     1081 gggtttgccc acatcttctt caacatgttc gctgtttgga tgttcggaac ggcgatcgaa
     1141 caggtttggg gaagccgtag gttcctcacc tactatctgc tgacgggtgt cggcgcggca
     1201 ctttgtcatt acgggttgtt ctatttcgaa ctgcagccgc tgcaagcgct ctttgaaacc
     1261 taccaacaat cccctaccgt tgagaacctc gagacactcc tgcacagtcc cttactccat
     1321 acgtattggt cggctgaagt ggaaagttcc atccgctcat tcgttgaaga attcaatgtg
     1381 cagtattcag ccgacccgga aaaagccatg atctacagtg tcggttacgt aaag
//
LOCUS       DAOTON010000794         1430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_71635_length_1430_cov_2.397818, whole genome shotgun sequence.
ACCESSION   DAOTON010000794 DAOTON010000000
VERSION     DAOTON010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71635_length_1430_cov_2.397818"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1430
                     /locus_tag="P5292_12825"
     CDS             <1..>1430
                     /locus_tag="P5292_12825"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS40056.1"
                     /translation="TTSLGDTVTEPDAMIASTNSVAVRCNGGADGAAEIAVNGGTPPY
                     NYEWAGIGVQQPLAANLRAGTYAVQVTDANGCTRTETVTVSETTALQFTSSASAVRCN
                     GGADGSVNVSIQGGTSPYTYLWSTGASSSSVDQLAAGSYSVSITDNNGCTIAGSSTVT
                     EPPTLLLSTQGDTWLCIGQSAQIQAIAAGGTPGYTFEWSNGIQGASQTVQPAVTSAYA
                     VTLTDANGCTAGPQTVVLQLYPALTAIVSNADTLCPGDPATLTAFANGSNGGPYSYTW
                     NTGALGPSITVTPATTTLYTVTVSDQCGSAPVQLQVPVNVVAAPSSNFLPNPASGCVP
                     FTVQYVNNGNLPAGTSYLWDFGDGATSTDRNPAHTFAQPGAYTVRHFVITPLGCASQT
                     LVPAAVNVHPNPIADFIAQPEEVSIFRPRVEMINNSLRSVRWNWDFGDGLGNSAEREP
                     VYTYRDTGNYTIRLVSRTEHGCPDTT"
BASE COUNT          309 a          424 c          408 g          289 t
ORIGIN      
        1 tactaccagc cttggcgata cggttacgga acccgatgcc atgattgcga gcacgaacag
       61 cgtcgcggta cgctgcaacg gcggagccga cggcgctgct gaaatcgctg tcaacggcgg
      121 aacgcctccc tataattatg aatgggcagg catcggtgtg cagcagcctc ttgcagccaa
      181 cctgcgcgcc ggtacctacg cggttcaggt caccgatgca aacggctgta cccgtacgga
      241 gacagtgacc gtttcggaaa ctacggcttt gcaattcact tcgagtgcgt cagcggtccg
      301 ttgtaatggc ggagccgacg gatcggtcaa tgtctcgatt cagggaggaa cgtcacctta
      361 tacctattta tggagtaccg gtgccagttc atccagtgtc gatcaactgg cagccggcag
      421 ctacagtgta agcatcacgg ataacaacgg ctgcaccatc gctggctcgt caactgtcac
      481 agagccgccc actttgttgc tttcaacgca aggcgacacc tggttgtgca tcggacagtc
      541 ggcacagatc caagcgatcg ccgccggagg caccccgggc tatacgttcg aatggagcaa
      601 tggaatacag ggagcgagtc aaacggttca acctgcggta accagcgcgt acgcggttac
      661 gctgaccgac gcgaacggtt gtacagccgg tccgcaaacg gtcgtgctgc aattgtatcc
      721 tgcactgacg gcgatcgtat cgaatgcaga tacactttgt ccgggcgatc cggcaacgtt
      781 aacggccttt gccaacggca gtaatggcgg gccgtattcc tacacttgga ataccggtgc
      841 gttgggacct tcgatcaccg tcacaccagc tacgactacg ctctatacgg tgacggtcag
      901 cgaccagtgc ggcagtgcgc cggtgcagtt gcaggtgccg gtgaacgtgg ttgctgcgcc
      961 ttcctcgaac ttcctgccga atcctgcgag cggttgtgtt ccgttcacag tacaatatgt
     1021 caacaatggc aacttgccgg caggaaccag ctatctctgg gacttcggtg atggcgccac
     1081 gagcaccgac cgtaaccccg cgcatacatt cgcgcagccg ggcgcgtaca cagtgcggca
     1141 cttcgtgatc acaccactgg gctgcgcgtc gcaaacgctc gtacctgccg cggtgaatgt
     1201 tcatccgaat ccgatcgccg atttcatcgc gcagccggag gaagtatcga tcttccgtcc
     1261 acgcgtggaa atgatcaaca acagcctgcg ttcggtacgc tggaattggg atttcggcga
     1321 cggcctcggc aactccgccg aacgtgaacc ggtctatact tatcgggaca ccggtaacta
     1381 tacgatccga ctggtcagtc gaacggaaca cggttgcccc gatactacct
//
LOCUS       DAOTON010000795         1428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_71790_length_1428_cov_2.065550, whole genome shotgun sequence.
ACCESSION   DAOTON010000795 DAOTON010000000
VERSION     DAOTON010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71790_length_1428_cov_2.065550"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="P5292_12830"
     CDS             complement(<1..930)
                     /locus_tag="P5292_12830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRS40057.1"
                     /translation="MGPKQPEISIIIPCYTYGRYLPETLKSLLDQTFSDWECVLVANG
                     SDALTLQVIQVFVDKDSRFRKLSTQNHGPSAARNEGIQASRGRYIQLLDADDILENRK
                     LEVQSSYLHAHPETGVVYGEVRYFTDGAPEQWRRSLHLPDREWMPKVSGKGSLLVNTL
                     LRYNIMTIHAPLVRREMMDLVGCMDEALSGFEDWDLWLRMAMSDVQFTFLPVAGSLSL
                     VRTHPGSLNQDRSTMRQYLLRVWEKALESGQLNLKQRVYVWSRMEEEFGEMVLARRTG
                     QAMLSPRSRLVLWAGVPFIYPVVKLGRYLRNLSK"
     gene            complement(933..>1428)
                     /locus_tag="P5292_12835"
     CDS             complement(933..>1428)
                     /locus_tag="P5292_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS40058.1"
                     /translation="NSGDYLASRDVLSAVFSRRRDEDILYGNMIIDYGNGKTRLGRMP
                     RRITLEQMIGDTLWHPVSFIRRTLFEKYGKYDLSFRVVADYHFFLRVLLVEQVSYYHT
                     GVTVSVFDTSGMSSQGKYRAQQLEERRRAQELYFKPEVIDRALHRYRRKFLLNRVLRR
                     IGLN"
BASE COUNT          365 a          415 c          324 g          324 t
ORIGIN      
        1 ctttgacagg ttacgcaaat aacgacccaa cttcactacc ggataaatga acggaacgcc
       61 agcccataag acaaggcggc ttcggggaga aagcatggcc tgaccggtcc ttcttgccag
      121 caccatttca ccgaactctt cttccattct tgaccatacg taaacacgct gcttcaaatt
      181 caactgtccg gattccaggg ccttctccca cacacgcaac aagtactgtc gcatggtcga
      241 tcggtcctgg ttgaggctgc caggatgcgt tctgacgagc gaaagcgacc cggcgaccgg
      301 aaggaaagtg aattgcacgt ctgacatggc cattcgtaac cagaggtccc aatcctcaaa
      361 ccccgacaat gcctcatcca tacagccgac cagatccatc atttcccgtc gaacgagcgg
      421 tgcatggatg gtcatgatat tgtaccgaag caatgtattc accagtaggc ttccctttcc
      481 ggaaaccttt ggcatccatt cccgatccgg aaggtgcagc gaccttctcc attgttccgg
      541 tgctccgtca gtaaagtaac gcacttcacc atacaccacg cctgtttccg gatgcgcatg
      601 taaataggaa gattgaacct cgagcttccg gttctccagt atatcatcgg catccaacaa
      661 ctgaatatag cgtccgcgcg atgcttgaat cccttcgttt cttgccgcgg atggcccgtg
      721 gttctgcgtg gacagtttgc gaaatcggga gtccttatcg acaaagacct gaataacctg
      781 cagtgtcaac gcatcagaac cattggcgac cagaacacat tcccaatcag aaaaagtctg
      841 atccaggagc gacttcaacg tttccggcag ataccttcca taggtatagc aaggaatgat
      901 gatggagatt tccggttgtt tcggtcccat gatcagttca atcctatcct gcgcaagacg
      961 cggttcaaca gaaacttgcg acgataccga tgcaatgccc ggtcgatcac ttccggctta
     1021 aagtacaact cctgtgcccg gcgtcgttct tccagttgtt gggcacgata cttcccctga
     1081 gaactcatgc cggaagtatc aaacactgat acggtgacac cagtatggta atagctcacc
     1141 tgctccacca ggagcacccg gagaaaaaaa tgataatccg ccaccacccg gaagctgagg
     1201 tcatacttac catacttctc aaacaaggtc ctgcggataa aactcaccgg gtgccaaagc
     1261 gtatctccga tcatctgctc cagggtgatt cttcgcggca tacggcccag gcgggtcttc
     1321 ccatttccat aatcgatgat catgttgccg tacaaaatat cctcatctcg tcgacggctg
     1381 aaaaccgcgc ttaatacatc gcgggaggcc agataatcgc ccgagttc
//
LOCUS       DAOTON010000796         1426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_71916_length_1426_cov_2.495988, whole genome shotgun sequence.
ACCESSION   DAOTON010000796 DAOTON010000000
VERSION     DAOTON010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71916_length_1426_cov_2.495988"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..>1426)
                     /locus_tag="P5292_12840"
     CDS             complement(153..>1426)
                     /locus_tag="P5292_12840"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRS40059.1"
                     /translation="IQNITSIKGINYVQSAFGQFNNLLNAAVDSAYIIITHRALWNQA
                     LAYKNYRNITTANRVVMVDIDELYDQFSYGIQKHPLSIRNFVRFALERWTGVGKPQHI
                     LLLGKSMSPADFRQNPTLFELCLVPSYGVPTSDMLLVTGLDGTLYEPSVPIGRICART
                     GNEILDYLAKVQEFEQVQSGPPQTWMKEILHFGGGNNQGQQDQLAGYLSTYEQIMEDS
                     LYGGHVTTYLKNSNDPIVINQSDTLQAQIDNGVSIMTFFGHASGSGFDQSTDEPSEYN
                     NQGKYPLIVANSCFAGDIHTYQKSVSEKFVLEPSKAAIAFIASVGQGIPEDLYAYSTA
                     LFQNTSRNHYGATIGQLMKYSIQDIQAPGMENIKIVCNEMSLNGDPALRLNVWTKPDY
                     MVDGSSLRFNPAAVTTDLDTFSVVVNVRNLA"
BASE COUNT          316 a          333 c          424 g          353 t
ORIGIN      
        1 agattcagtc cggctgaact gaacccgccg gtggtcatgg tgatcgaaaa ctcgtcgtta
       61 tagaagcaat taccgcgttg gaaggtgtaa acggaatccg aaccgtccgg ataggtacgg
      121 gtgacacgaa ccgtgaagga atcagagacc gcttaggcga gatttcggac gtttacgacg
      181 acggaaaacg tatccagatc ggtcgtaacg gcggcaggat tgaaacgcag cgaactgcca
      241 tcaaccatgt aatcgggttt tgtccagaca ttcagccgca gtgccgggtc gccattcagc
      301 gacatctcgt tgcagacaat cttgatgttc tccatgcctg gcgcctggat gtcctggatg
      361 gagtatttca tcagttgtcc gatcgtagcg ccatagtggt tgcggctcgt attttggaac
      421 aaggccgtcg aataggcata caggtcttcc ggaataccct ggccgacact tgcaatgaac
      481 gcgatggcgg ctttgctggg ttcgaggacg aatttttcgg aaacgctttt ttggtaggtg
      541 tggatgtcgc cggcaaagca ggagttggcg acgatcaacg ggtacttacc ctggttgtta
      601 tactcggaag gttcgtccgt actctggtcg aatccggagc ccgaagcgtg tccgaagaag
      661 gtcatgatcg agaccccatt gtcaatctgt gcctgcaggg tatcggattg attgatgacg
      721 atggggtcgt tgctgttctt gagataggtg gtaacatgtc ctccgtaaag actgtcttcc
      781 atgatctgct cgtaggtgct caggtagccc gcaagctggt cctgttgccc ctgattgttg
      841 ccgccaccga aatgcaggat ctctttcatc caggtttgtg gcggaccgga ttgtacttgt
      901 tcgaattcct ggaccttggc caggtaatcg aggatctcat ttccggttcg ggcgcaaatc
      961 cggccgatcg gaaccgatgg ttcatacaag gtgccgtcaa gtccggtcac gagtagcatg
     1021 tcggacgtgg gtactccata ggaggggacc agacacaatt cgaagagtgt cgggttctgg
     1081 cggaagtcgg caggactcat cgacttaccc agtaacagga tgtgctgcgg tttccccact
     1141 ccggtccagc gttccagtgc aaagcgaacg aagttgcgga tcgacaaggg atgtttctgg
     1201 ataccgtagg agaattggtc gtacaattcg tcgatatcga ccatcacgac ccggtttgcg
     1261 gtggtgatat ttcgatagtt cttataagct aatgcctggt tccagagcgc gcgatgggta
     1321 atgatgatgt aagcggaatc cacagcggcg ttcaggaggt tgttgaactg gccgaaggcg
     1381 ctttgaacgt aattgatccc tttgatcgaa gtgatgttct ggatcg
//
LOCUS       DAOTON010000797         1423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_72130_length_1423_cov_5.233918, whole genome shotgun sequence.
ACCESSION   DAOTON010000797 DAOTON010000000
VERSION     DAOTON010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72130_length_1423_cov_5.233918"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..>1423
                     /locus_tag="P5292_12845"
     CDS             60..>1423
                     /locus_tag="P5292_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40060.1"
                     /translation="MYASFDPCWNSRNARACTLLVVLLCLVRPSLSAQTIYLSEDFSA
                     GSLPSGWTVFNQQSPCIGWEFGDSLESAGFPIPPHGMYAAVNDNRYDNAAGTANLANW
                     CYLYSPIIDCSVADSLRLEFDYFIPPDIDGRANVALSNGGIYNYFFSINEQNGWNRFR
                     WNIPRAYYTANFRFRIGFSDNGASANGLAIDHVVLSDPGQFDLGIVRPYFGTVVPVSG
                     ARTGMKLRNNGLDTITDFRLDVTVDGTPAFSESFGGLSLLYARDFDCMPATRLPSLGP
                     GRQRIGFALRAVNGQPLDENPSNDTASFVVEVAPNVPDRRPVLIDKTGAWCTYCADGS
                     MQLDSAVVWNPEAIGVAIHGGDMMDSSSSSVFTGTVFTNALMNGGYPKLSCDAYKFPE
                     LPTLTTEPYNYGTLLAERLVSYEPVGVKLEGIQWDVTHRTVSATVVVEAYDTITADLR
                     PNLWV"
BASE COUNT          275 a          449 c          389 g          310 t
ORIGIN      
        1 gcttcttcct atattcgtta gaagcccgac tctcttccac cggacaaatg gatttaacca
       61 tgtacgcaag ttttgatccg tgttggaata gccgtaatgc ccgggcatgc acgcttcttg
      121 tggtgctact gtgccttgta aggccgagct tatcggcgca gacgatttac ctgtcggaag
      181 acttttccgc cggcagtttg ccgtctggct ggacggtctt caatcaacag agtccttgca
      241 tcggctggga gtttggtgac agcctggaat ccgccggctt cccgatcccg ccgcacggca
      301 tgtacgccgc cgtgaacgac aaccgttacg acaacgccgc gggcaccgcc aacctggcca
      361 actggtgtta cctgtactcg ccgatcatcg attgctccgt cgccgactcg ttgcgactgg
      421 agttcgacta tttcattccg cccgacatcg acggacgcgc caacgtggca ttgtcaaacg
      481 gtggtatata caattacttc ttcagcatca acgaacagaa cggctggaac cgttttcgct
      541 ggaacatccc gcgtgcctac tacacggcta atttccgatt ccgcatcggg ttcagcgata
      601 acggcgcttc cgccaacgga ctggcaatcg accatgtagt actgagcgat cccggccagt
      661 tcgacctcgg catcgtacgg ccttacttcg gcactgtcgt tccggtcagc ggcgcacgca
      721 ccggcatgaa gctgcgcaac aacggccttg atacgatcac cgactttcgt ctcgatgtta
      781 ccgtggacgg aactcccgcg ttttccgagt cctttggagg actctcgctg ctttacgcgc
      841 gcgattttga ctgtatgccg gccacgcgcc tgccctcgct cgggcccgga cgtcagcgca
      901 tcggcttcgc gctgcgggcc gtgaacggcc agccgctcga cgagaaccct tccaacgaca
      961 cagccagctt tgtcgtggaa gttgccccga acgtgcccga ccgacgaccg gtgctgattg
     1021 acaagaccgg ggcctggtgt acctattgcg ccgacggcag tatgcaactc gactccgcgg
     1081 tggtctggaa ccctgaagcc atcggcgtag ccattcacgg tggcgacatg atggattctt
     1141 ccagctcttc cgttttcacc ggcacggtct tcaccaacgc gctgatgaac ggaggttatc
     1201 ccaagctgag ctgcgacgca tacaagtttc ccgaactgcc cacgctcacc accgaaccat
     1261 acaattacgg caccctgctc gcggaacggc tcgtcagcta cgagccggtc ggcgtgaagc
     1321 tcgaagggat ccagtgggat gtgacgcacc gtacggtcag tgcgaccgtg gtagtcgagg
     1381 cgtacgatac gatcacagcg gatttgcgcc ccaatctctg ggt
//
LOCUS       DAOTON010000798         1423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_72153_length_1423_cov_2.693713, whole genome shotgun sequence.
ACCESSION   DAOTON010000798 DAOTON010000000
VERSION     DAOTON010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72153_length_1423_cov_2.693713"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..>1423)
                     /locus_tag="P5292_12850"
     CDS             complement(266..>1423)
                     /locus_tag="P5292_12850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40061.1"
                     /translation="VAGSTTIPNNTGINAIELRPGSRFIGNHLHDFYTIGNFSGNNTG
                     IGIDLSQGLEIAGNFIHHFEAVAPYTPSRGIAYNETISSYPQYDHWIYNNMIALGFDS
                     LGNELTTPLEAYGIYGVVDSTIITHNSIFLAGTADGNSAGIRVTESGTHMSRITNNIL
                     VNKKNQGTPGSFYYDNNITLDAGVTASGTLSIDYNLCDLRASGRSNLLQDANQYYPDF
                     VSIRNATPYDDHSILGNPSFRNAPGTNQSVDLHVNYPSPADASGIPEPSVLIDYDGEI
                     RNLFTPVDIGADAISSTTGVSPGLGANTRLPMILAPNPAGENVFVLDSEGKLQAASVR
                     IIATDGRLLDPRQTGSFPSLQLDLRGIPAGCYIVSATDAEGIEYRSILIRR"
BASE COUNT          335 a          320 c          399 g          369 t
ORIGIN      
        1 tcactatgtg taaatacgag atagacgtaa tccttttttt tagaaaaaaa cttgtcggaa
       61 tttttgcgaa accaatgtgc aaaaccatta gtaagcaaag agtgattgat atcaagcgat
      121 caggacaaca agattacagg attgcctgtt cgagcgtacg ctggataccg aagctccccg
      181 acttataggc agactttaat acctgtaggg ttcatgacgg ctccggtgcc tggtatttta
      241 caactctatt gaaaagctga tgcgcctagc gcctgatcag gatactacga tattcgatac
      301 cctccgcgtc ggtggcactg acgatgtaac aacctgcggg aatcccgcgg agatcaagtt
      361 gtagtgatgg gaatgagccg gtttgccggg gatccagtag cctgccatcc gttgcaatta
      421 ttcgtaccga ggctgcctgc aatttccctt cgctatcaag gacaaaaaca ttttcacctg
      481 ccggattggg tgcaaggatc atgggcaatc gggtgttagc cccaagccct ggtgaaacac
      541 ctgtggtgga agagatcgca tctgctccga tgtccaccgg ggtgaacagg ttgcggattt
      601 cgccatcgta gtcaatgagc acagaaggtt ccggtattcc ggaggcatcg gccggactgg
      661 gataattcac gtgcagatcg acggattggt tcgtgccggg agcgtttcga aagctcggat
      721 tgcccaggat gctgtggtcg tcgtacggtg tcgcgttgcg gatggaaacg aaatccggat
      781 agtattggtt cgcatcctgt aacagattgg aacgaccgga agcacgcaaa tcacataggt
      841 tgtaatcgat cgacaaagtt ccggaggctg tcacaccggc atcgagggtg atgttgttgt
      901 cgtagtaaaa gctgccaggt gttccctggt ttttcttgtt tacaaggatg ttgttggtga
      961 tccgggacat gtgcgttccg gattccgtta cccggattcc tgcagagttc ccgtcggcgg
     1021 ttccggcaag aaagatcgaa ttgtgggtaa tgatggtgct gtctaccact ccatagatcc
     1081 cataagcttc caaaggggtt gtcaattcgt tgccaagact gtcgaatccc aaggcgatca
     1141 tgttgttgta aatccaatgg tcatattgag ggtagctgga tatggtctcg ttgtaggcga
     1201 ttccacgcga aggtgtataa ggtgctactg cctcaaaatg atgaatgaaa ttccctgcga
     1261 tctcgagtcc ctggcttagg tcgatcccga ttccggtatt gttgccgctg aagttgccga
     1321 tggtgtagaa gtcgtgcagg tggttgccga tgaaccggct acctggccgc aattcgatcg
     1381 cgttgatgcc ggtattgttc ggaatggtag ttgaacccgc tac
//
LOCUS       DAOTON010000799         1422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_72220_length_1422_cov_2.507681, whole genome shotgun sequence.
ACCESSION   DAOTON010000799 DAOTON010000000
VERSION     DAOTON010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72220_length_1422_cov_2.507681"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..>1422
                     /locus_tag="P5292_12855"
     CDS             112..>1422
                     /locus_tag="P5292_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40062.1"
                     /translation="MTFADAMVDFQGDLYSIQVSGTGSIWKYDVSLDSWGLLSGNFTP
                     DNRAGAVIFLDGSKIWVVNGKVSGTSPLSYLNTGYYFDLLTGLRSPAASLFSSTGTTN
                     TTAFATGMKIDGRFYYCFGQFLSSKSTAICRYEQLACTTLPYYVPVEIDEAGIGVPHV
                     FSNYSFLQLPEDTSQLSLLVDGQSVTPVNGFETTLEHTFRQCGEHQLRFIVTGSGCAD
                     TATYYQTAHPIYNVESRAEMPLGQYNIRHTMNAFSIGNTGYMGMGNKYVDILVEAYKD
                     WYAYDPASDTWSVRASLPAADDRTGSACFANNNYGFISSGNKGCFGSMSCYFNETWKY
                     DPALDVWTQQSPIPGIGRYELCATVLGDTAYAGLGYYPFNNPGQIDKYSFSSDTWTSS
                     GWPYGKSGSATPIAFTWNGKVYAGYNVLMDGGPYPGLYAYDPATG"
BASE COUNT          322 a          354 c          373 g          373 t
ORIGIN      
        1 gctgcgagcg taatcaatga ccaggtgtat ctatgtttcg gaggcgcggc agagttgtat
       61 cggtacgata taccttcttc gacatggaca gcgttggcat ctcatccaac cttgaccttc
      121 gcggatgcta tggtggactt tcagggtgat ctctattcca tacaagtgtc gggtaccggt
      181 tcgatatgga agtacgatgt ttcactcgat tcctggggac tattatccgg caacttcacg
      241 ccggataatc gggccggcgc ggtcattttt ttggatggaa gtaaaatctg ggtggtcaat
      301 gggaaagtaa gcggcacctc cccattatcc tatctgaata cgggatatta ctttgatctc
      361 ctcacagggt tgcggagtcc tgcggcctcg ctcttttctt caacaggaac tacgaacacg
      421 actgcatttg ccaccgggat gaaaatcgat gggcgattct attattgttt cggtcagttt
      481 ctcagttcca agagtacagc catttgtcgt tacgaacagc ttgcctgtac caccttgccc
      541 tattatgttc cggtggagat tgatgaagcc ggaatcggcg taccgcatgt attctcaaat
      601 tatagtttcc tgcagttacc ggaagatact tcgcagttat ccttattggt tgacgggcag
      661 tcggtgactc cggtgaatgg ctttgagacc accctggagc atacgtttcg ccaatgtgga
      721 gaacatcaac tccggttcat cgttaccgga tccggctgtg ccgatactgc aacctattat
      781 cagaccgctc atccgattta taatgtggaa tctcgggcag agatgccctt gggacaatac
      841 aatatccgtc acaccatgaa cgcgttcagc atcggcaata ccgggtacat gggcatggga
      901 aacaagtacg ttgatattct ggtggaagcc tacaaggatt ggtacgctta cgaccccgca
      961 tcggatacct ggtcggtgcg cgcttcattg cccgcggcgg atgaccggac cggctcagcc
     1021 tgttttgcca acaacaatta tggatttatt agcagcggaa acaaaggttg cttcggcagt
     1081 atgtcctgtt acttcaacga gacttggaaa tacgatccgg ccctggatgt gtggactcag
     1141 cagtcgccga tccccggtat tggccgttac gaactgtgcg cgacagtact gggcgatacg
     1201 gcttatgccg gcctgggtta ttatccgttc aacaatcccg ggcagattga taaatattcg
     1261 ttctcttccg acacctggac gagttcggga tggccttatg gcaagtccgg ctcagcgaca
     1321 ccgatcgcgt tcacctggaa cggtaaagta tacgccgggt ataatgtact catggatgga
     1381 ggaccgtacc cgggtctcta cgcatatgat ccagcaaccg gt
//
LOCUS       DAOTON010000800         1417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_72597_length_1417_cov_2.989721, whole genome shotgun sequence.
ACCESSION   DAOTON010000800 DAOTON010000000
VERSION     DAOTON010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72597_length_1417_cov_2.989721"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="P5292_12860"
     CDS             <1..532
                     /locus_tag="P5292_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40063.1"
                     /translation="ERLPELSVSPEESHDTAKSPETDTTKPKAESGNFFAWLRSKQVD
                     GFGAVEAVHAEDDQKDEREPIKAATLPGTVIPAKATDEQSALIDKFIAEEPRIVPSKT
                     EFFNPVVQAKRSVEEHEDIVSETLAGIYAAQGNLLKARSAYQRLGLLHPEKSAYFAAL
                     VREIDEKLNSSSSEDL"
     gene            537..914
                     /gene="secG"
                     /locus_tag="P5292_12865"
     CDS             537..914
                     /gene="secG"
                     /locus_tag="P5292_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRS40064.1"
                     /translation="MFTAITVLIIIICILLVLVVLVQNPKGGGVASGIIGANQLMGVK
                     KTTDFIEKLTWGLVIGLVSLCLLAGFALPKKGDREIGTKMQEQIDAGAAPTTTPAAPQ
                     QPAQQPAQQQPAQGEPAQQQPAQ"
     gene            1166..>1417
                     /locus_tag="P5292_12870"
     CDS             1166..>1417
                     /locus_tag="P5292_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRS40065.1"
                     /translation="MSKLTLKPLGDRVLVEAAAAEEKTASGIIIPDTAKEKPQRGKVV
                     AIGTGKKDEPMTVKVGDSVLYGKYAGTEITIDGRELLIMR"
BASE COUNT          392 a          396 c          351 g          278 t
ORIGIN      
        1 ggagcgatta ccggaacttt cagtaagtcc tgaggaatca cacgacactg ccaaaagtcc
       61 ggaaaccgac acaactaaac ctaaggcgga aagcgggaac ttcttcgcct ggctgcgatc
      121 gaaacaggtc gatggttttg gtgccgtcga agctgttcat gctgaagacg atcaaaagga
      181 cgagcgggag ccgatcaaag cggctaccct acccggcact gtcattcctg caaaagccac
      241 cgacgaacag agtgccctga ttgataaatt catcgcggaa gagccccgga tcgtaccgtc
      301 aaagaccgag ttctttaacc ccgttgtaca ggccaaacga agcgttgagg aacacgaaga
      361 cattgtttct gagacacttg caggcattta cgccgctcag ggaaatttac tgaaggcgcg
      421 atccgcctac caaagactgg gcttgcttca tccggaaaaa agcgcttact ttgccgccct
      481 tgtccgcgaa atagacgaaa aacttaacag ttcgtcgagc gaagacctat aaagcaatgt
      541 ttaccgccat taccgtcctc atcatcatca tttgcatcct ccttgttctg gtcgtgcttg
      601 tccagaaccc caaaggcggc ggcgtcgcgt ccggcatcat cggagccaac cagctcatgg
      661 gcgttaaaaa aacgacggat ttcatcgaaa aacttacctg gggcctcgtt atcggcctgg
      721 tatccctttg cctgttggca ggttttgcct tgccgaagaa gggcgatcgc gagatcggta
      781 ccaagatgca ggagcagatc gacgcgggcg ccgcgccgac caccactccg gcagctccgc
      841 agcaaccggc acaacagccg gcgcagcaac aacccgccca gggagaaccg gcacaacagc
      901 agccggctca gtaatgaaac tgaactgaat cgtcgaaaag ctgcctccat ggcggctttt
      961 cgcgtttttc ggcaccgaca aaaaccgaaa tccctcagcg ctgaaagttt gtcacttaaa
     1021 tccctgacac ctcttccttg gatttcaaaa agattgaaat tttgtcagga aacgaagaaa
     1081 atataaccac tggcacgttt tctgaccacg gcggtcgacg taagtcagtc aactctattt
     1141 gtccaacctt aaaatcaata cactcatgag caaactcacc ctgaagcccc tgggcgatcg
     1201 cgtgctggta gaagcagcag cggcagaaga aaagaccgca tccggaatca tcattccgga
     1261 caccgcgaaa gaaaaaccgc agcgtggtaa agtcgtagct atcggcacgg gtaagaaaga
     1321 cgagccgatg accgtaaaag taggcgactc ggttctttat ggtaaatatg ccggtaccga
     1381 aatcaccatc gacggacgcg agctcctcat catgcgc
//
LOCUS       DAOTON010000801         1414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_72799_length_1414_cov_3.647535, whole genome shotgun sequence.
ACCESSION   DAOTON010000801 DAOTON010000000
VERSION     DAOTON010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72799_length_1414_cov_3.647535"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="P5292_12875"
     CDS             complement(<1..134)
                     /locus_tag="P5292_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRS40066.1"
                     /translation="MEAVVVNTAGKETGKKVRLNDQIFGIEPNDHAIYLDVKQYLANQ
                     R"
     gene            complement(136..753)
                     /gene="rplC"
                     /locus_tag="P5292_12880"
     CDS             complement(136..753)
                     /gene="rplC"
                     /locus_tag="P5292_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRS40067.1"
                     /translation="MSGIIGKKIGMTSVFSADGTYIPCTVIEAGPCVVTQVKTKEKDG
                     YTAVQLAFGDKKEKHTTKAEAGHFKKSGTSPKRNVMEFRHFEQLPEVGSEIKADLFAE
                     GDFVDVVGTSKGRGFQGVVKRHGFSGVGMQTHGQHNRLRAPGSRGASSFPSRVWPGQR
                     TAGHMGVARVKMQNLQVVKVVPEQNILVVKGAVPGHKGSIVIVEK"
     gene            complement(817..1125)
                     /gene="rpsJ"
                     /locus_tag="P5292_12885"
     CDS             complement(817..1125)
                     /gene="rpsJ"
                     /locus_tag="P5292_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRS40068.1"
                     /translation="MSQRIRIKLKSYDHNLVDKSAEKIVKTVKVTGAVVNGPIPLPTG
                     KKIFTVLRSPHVNKKAREQFQLCSYKRLLDIYNSTAKTVDALMKLELPSGVEVEIKAS
                     "
     gene            complement(1168..>1414)
                     /gene="fusA"
                     /locus_tag="P5292_12890"
     CDS             complement(1168..>1414)
                     /gene="fusA"
                     /locus_tag="P5292_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667230.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRS40069.1"
                     /translation="MGDLNKRRGQLEGMDTRAGAQVIKAKVPLSEMFGYVTSLRTITS
                     GRASSSMEFSHYSECPRTLAEEVIAKAKGKAKAEKVN"
BASE COUNT          270 a          398 c          384 g          362 t
ORIGIN      
        1 cgctggttgg cgaggtattg cttcacgtcg agatagatcg cgtgatcgtt cggctcgatc
       61 ccaaagatct ggtcgttcaa ccggaccttc ttgccggtct ctttaccggc ggtgtttacc
      121 actactgctt ccatcttatt tctccacaat tacaatggaa cctttatgtc ccggaacagc
      181 acccttcaca acgaggatgt tctgctcagg aaccactttt actacctgca ggttctgcat
      241 cttcacacgc gctacgccca tgtgaccggc agtacgctgg cccggccata cgcgggacgg
      301 gaacgaggac gctccacgcg aacccggggc acgaagacgg ttgtgctgac cgtgggtctg
      361 cataccgaca ccgctgaagc cgtgacgctt cacaacgccc tggaaaccgc ggcccttcga
      421 ggtgccaacg acatccacga aatcgccttc tgcaaagaga tcggctttga tctcgctgcc
      481 cacttccgga agctgttcga agtgacggaa ctccatcacg ttacgcttcg ggctggtgcc
      541 tgatttcttg aaatgaccgg cttccgcctt ggtcgtgtgc ttttctttct tgtcgccgaa
      601 agccagctga acagccgtgt agccgtcctt ctccttggtc ttcacctggg tgacgacgca
      661 aggacccgct tcaatcacgg tacacgggat gtaagtcccg tccgcgctga agacgctggt
      721 cattccgatc ttctttccga tgattcctga catggtgtta gtactgttgt gccagcggtt
      781 tggagcacgt tccaaacata gccgttgttg aattgcttag gatgctttga tctctacttc
      841 cacaccgctc ggaagctcca gcttcatcaa cgcgtcgacg gtcttggcag tcgagttgta
      901 gatgtcgagc aggcgcttgt atgagcacaa ttggaactgc tcgcgagcct tcttgttgac
      961 gtgcggggaa cgcagcacgg tgaagatctt cttgccggtc ggcagcggaa tgggtccgtt
     1021 gacgactgcg ccggtgactt tcaccgtctt cacgatcttc tcggccgatt tgtcaaccag
     1081 attgtgatcg taggacttga gcttaatgcg gatgcgctga gacatgttga aagaacgttg
     1141 gtaggatatg tcttgttgag gtattgctta gttgactttc tcggcttttg ctttgccttt
     1201 cgccttggcg atgacttcct cggccagggt acgcgggcat tcgctgtagt gtgagaactc
     1261 catgctggag gaagcacgac cggaggtgat ggtacgcaac gaggtcacgt aaccgaacat
     1321 ttccgacagc ggaactttcg ccttgatcac ctgcgcgccg gcacgggtat ccatgccttc
     1381 gagctgtcca cgacgcttgt tcaagtcgcc catg
//
LOCUS       DAOTON010000802         1411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_73080_length_1411_cov_2.381268, whole genome shotgun sequence.
ACCESSION   DAOTON010000802 DAOTON010000000
VERSION     DAOTON010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73080_length_1411_cov_2.381268"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="P5292_12895"
     CDS             complement(<1..191)
                     /locus_tag="P5292_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRS40070.1"
                     /translation="MDKELDDFYAAYPDYGSLSPLTIKRYRELLQNFEPFRKNNRLLE
                     VGCGHGAFLELARQEGWEVT"
     gene            complement(343..975)
                     /locus_tag="P5292_12900"
     CDS             complement(343..975)
                     /locus_tag="P5292_12900"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HRS40071.1"
                     /translation="MILSHQKKFLFIHIYKVAGTSIRRVLDRYDDRTWNDFPFLTNVK
                     FKIGSRSKRLSTWAIDHITASQCKDWLSPEVFNQYFKFSFVRNPWDWQVSLYHFMLQD
                     KHHRQHKLISKTKNFEEYLTWRVNKDIELQTDFLYDKSGTLLVDYIGRFETLQSDFNE
                     ICRRLNIQSTQLPITNRSHHQYYKEYYNDRTRDMVANAFKKDIELLGYEF"
BASE COUNT          369 a          307 c          357 g          378 t
ORIGIN      
        1 gtcacttccc agccttcctg acgggctaat tcgagaaacg ccccgtggcc gcagcccact
       61 tctagtaatc ggttgttttt tctaaaaggt tcgaaatttt gcagcaattc ccggtagcgc
      121 ttgatcgtga gcggagacaa agagccgtaa tcaggatatg ccgcataaaa gtcatccagt
      181 tctttatcca acggcatggg atgcatgaac gcatgccgac acttggggca ttcaaatagt
      241 ctgggaaatc tatcagggta gggtagttta cttgccgttg ctttacatac cggacatgtc
      301 ctcgagaggc gttggtccgc aatactggac aacttcatag catcagaatt cgtaccccaa
      361 aagttcgatg tctttcttaa aggcatttgc taccatatcg cgcgttcgat cgttgtaata
      421 ctccttataa tattggtgat ggctgcgatt tgttattggg agctgagttg attgaatgtt
      481 cagtcgccta cagatttcat taaaatcgct ttggagggtt tcgaatcgac ctatataatc
      541 gactaaaagc gtcccacttt tatcgtaaag aaaatccgtc tggagttcaa tgtccttatt
      601 tacgcgccaa gtaagatatt cttcgaaatt cttggtcttg ctgatcaact tgtgttgtcg
      661 gtgatgtttg tcctgcaaca tgaagtgata aagggaaacc tgccagtccc aggggttacg
      721 gacaaacgaa aacttgaagt actggttaaa aacctccggg ctcagccagt ctttgcattg
      781 gctggctgta atgtgatcga tcgcccaggt ggataagcgt ttggaacgac tgccgatctt
      841 gaacttgaca ttggtgagga aggggaagtc attccaggtc cggtcatcgt agcggtccaa
      901 tactcgacga atactggtgc cggctacttt gtagatatga atgaacagga acttcttttg
      961 gtgggaaaga atcataggac gtcgggactg acgagtcgca gttattcaga ctttgtggtt
     1021 accgtgtcct tgggtcgctt ccgttccgaa ttgaaatagg ataatgtgcc acgccggtaa
     1081 tcctttggaa attgggcagg aagttgaggc aaaagggggt tttcgggttc ggaagccgaa
     1141 gctgctttag cgccgcgccg agaacggctg aattcaaccg gtagggaggg aatgagacga
     1201 aaaatcatgg ccgcattaag atgaacattc agttaagggt gctgaccgtt tcaatcactt
     1261 cccgcgaccg caccagccgc tgaaatcggt aatcgattgg aacaaacgat caaaagcttt
     1321 ccgttctcga aacaattgcg aaaccaatgc ccattcatag ttggcataga ccctgttcac
     1381 aaatcgatcg atacgaggtg aaaagatact c
//
LOCUS       DAOTON010000803         1410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_73166_length_1410_cov_2.573432, whole genome shotgun sequence.
ACCESSION   DAOTON010000803 DAOTON010000000
VERSION     DAOTON010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73166_length_1410_cov_2.573432"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="P5292_12905"
     CDS             complement(<1..987)
                     /locus_tag="P5292_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40072.1"
                     /translation="MPRSGFKLDFQYGYTLLFSLLLGGMTASAQTVLLDDFNRANNNT
                     VGNGWNETETVAGTGATISGNNLRLASTTSGKDYVWRDVSTYYNTVLNTNTGTLTWAF
                     NLRQSRTDPSGFDAGNFGIAFVLGASSSNFLSGSSGYAVVLGNSLSGDNLRLVRFASG
                     MNTSAALTNVISPAIDYANQYMAVRVTYNPVGNAWTLEADTTNAGVFEDPVSGATYVS
                     LGSASDATYTGVDLMYLGCLWNHNTAAGDAGLFDNIYVPSACSPSPEPTTQATNVTFT
                     SIGAGAMTVNWTRGNGTFVIVIGRQSGAVTSTPTDGASYSASSTWGSGTLMAA"
     gene            complement(1172..>1410)
                     /locus_tag="P5292_12910"
     CDS             complement(1172..>1410)
                     /locus_tag="P5292_12910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40073.1"
                     /translation="PGQLDALQVYPNPAQQELRINSAERIDVRWFDETGMLVRTDRLF
                     AGTNRIAPGLPAGIYFLRFERDGLMQTIRVILLP"
BASE COUNT          329 a          359 c          411 g          311 t
ORIGIN      
        1 cgcggccatg agcgttcctg aaccccaggt ggagctggcg gaataggaag ctccgtcagt
       61 tggcgtactg gtgacggccc cactctgtcg accgatcacg atgacgaagg ttccgttacc
      121 acgcgtccag ttgacggtca tcgcaccggc gccgatcgac gtaaaggtca cattggtcgc
      181 ttgcgttgtg ggctccggac tgggactgca tgcggaagga acatagatgt tatcaaacag
      241 gccggcatcc cccgctgcgg tgttgtgatt ccagagacaa cccaggtaca tcagatcgac
      301 tccggtatag gtggcatcgg atgcggaacc gagcgaaacg taggttgctc cgctgacagg
      361 atcctcgaat acgcccgcgt tcgtggtatc ggcttccagg gtccaggcgt ttcctacggg
      421 gttatatgtg acccgcaccg ccatgtactg gttggcataa tcgatcgccg gtgagatgac
      481 gttggtcagg gcggcgctgg tgttcatccc gctggcgaag cgcaccaggc gcaggttgtc
      541 gccggacaag gagttaccca ataccaccgc gtatccgctc gaaccgctta gaaagttact
      601 ggaagaagcc ccgaggacga acgcgattcc gaaattaccc gcgtcgaatc cgctaggatc
      661 cgttcgcgac tggcgcagat tgaatgccca tgtcagtgtt ccggtattgg tgttcagtac
      721 cgtattataa taggtactga catcgcgcca gacgtagtct tttccggaag tggtactggc
      781 taatcgcagg ttattgccac taatggtagc gccggtacct gcaacggttt cggtctcatt
      841 ccagccattg ccgaccgtat tattgttagc ccgattgaag tcgtcgagca acacggtttg
      901 cgcgctggcg gtcatgccgc cgagcaacaa gctaaaaagc agggtgtagc cgtattgaaa
      961 gtcgagtttg aaacctgaac gaggcatgag gtgctacccg ttttaacaat tacgcaggca
     1021 gagggtttcg ggttgtaaaa gtattgaaat gagcccttta aaacctgaaa atgggatgag
     1081 caacgtaaaa atgatggtgg aatagccgaa atggacggct aaataccgtt ttgagtactc
     1141 acttaatgaa gaattaatgc ggaatgcgat gttacgggag taagatgacg cgaatcgttt
     1201 gcatcaatcc gtcacgttca aagcgcaaaa agtagatgcc agcgggcagg ccgggagcga
     1261 tccgattcgt tcccgcgaat aaccgatccg tccgaaccag cataccggtt tcatcgaacc
     1321 agcgtacgtc gattcgctcc gcgctgttga tgcggagttc ctgttgcgcc gggttgggat
     1381 aaacctgcaa agcatccaac tgtccgggtg
//
LOCUS       DAOTON010000804         1408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_73284_length_1408_cov_3.742794, whole genome shotgun sequence.
ACCESSION   DAOTON010000804 DAOTON010000000
VERSION     DAOTON010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73284_length_1408_cov_3.742794"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="P5292_12915"
     CDS             complement(<1..285)
                     /locus_tag="P5292_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HRS40074.1"
                     /translation="MSNNKRSALGRGLSALLESAETDVTTRSSGANASTPTGVVGSVA
                     VIPLEQIEPNPFQPRTEFKPEELKELSQSIKEQGIIQPITVRKLGYDKYQI"
     gene            complement(275..1090)
                     /locus_tag="P5292_12920"
     CDS             complement(275..1090)
                     /locus_tag="P5292_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRS40075.1"
                     /translation="MGKIIAIANQKGGVGKTTTAMNLAASLAVLEFKTLLIDSDPQAN
                     ATSGVGFDPRTIKTSIYECIINEVNPKDIIQKTTTPNLFLIPSHIDLVGAEIEMIHLP
                     NRERKMKEVLEQVKDQYDFIIIDCSPSLGLITINALTAANSVIIPVQCEYFALEGLGK
                     LLNTIKIVQSRLNPELEIEGILLTMYDSRLRLSNQVVEEVKTHFQQMVFDTIIQRNTK
                     LGEAPSFGQTIIMHDATSKGSVNYLNLAREILQKNNLTKIKESDKKIPVTDEQ"
     gene            complement(1134..>1408)
                     /locus_tag="P5292_12925"
     CDS             complement(1134..>1408)
                     /locus_tag="P5292_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRS40076.1"
                     /translation="YAGDTALHLDMKLIGDYKKMDLAFLPIGNNFTMGIDNAVIASDF
                     IRCDRIIGMHYDTFDMIKIDHDEAKKKFDSAGKQLTLMQVGQTLEH"
BASE COUNT          248 a          352 c          419 g          389 t
ORIGIN      
        1 gatctggtac ttatcgtagc cgagtttgcg gaccgtgatc ggctggatga tgccttgttc
       61 cttgatcgat tgggaaagct ctttcagttc ctccggcttg aactcggtcc ggggctgaaa
      121 agggttcggt tcgatctgct ccagtggaat gacggccacg gaacccacta cgccggttgg
      181 cgtggaagca ttggctcctg atgaacgggt cgtgacgtcg gtctcggcgc tttcgagcag
      241 ggcgctcagt ccgcgaccca gtgcgctgcg tttgttattg ctcatcggtc accgggattt
      301 tcttgtccga ctccttgatc ttggtcaggt tgttcttctg caggatctcg cgggcaaggt
      361 tgaggtagtt gaccgaacct ttgctcgtcg cgtcgtgcat gatgatggtc tgaccgaaac
      421 tcggcgcttc gcccaatttg gtgtttcgct ggatgatcgt gtcgaatacc atctgctgga
      481 aatgggtttt cacttcttcc accacctggt tactgagtcg cagacgggaa tcgtacatgg
      541 tcagcaggat gccttcgatc tccagttcgg ggttcagacg gctctgtacg atcttgatcg
      601 tgttgagcag tttgcccaga ccttccaggg cgaagtactc gcactgtacc gggatgatca
      661 ccgaattggc cgccgtcagg gcgttgatgg taatgagacc cagcgatgga gaacagtcga
      721 tgatgatgaa atcgtattgg tccttcacct gctcgagcac ttctttcatc ttccgctcgc
      781 ggttgggcag gtggatcatt tcgatctccg cgccgaccag gtcgatgtga gacggaatga
      841 ggaagaggtt cggggtggtg gtcttctgga tgatatcctt cgggttcacc tcgttgatga
      901 tgcactcgta aatgctcgtc ttgatcgtgc gcgggtcgaa gccgacgccg gaggtggcat
      961 tcgcctgcgg atcgctgtcg atgagcagcg tcttgaactc caataccgcc aggctggcgg
     1021 ccaggttcat cgccgttgtg gtctttccta cgccgccttt ctggttggcg atggctatga
     1081 tcttgcccat atcgtgtgtt tcttttatcg ttgctggtgg agctaccgta ggatcaatgc
     1141 tccagtgttt gaccgacctg catcagggtg agttgcttgc cggctgaatc gaatttcttt
     1201 ttcgcttcgt catgatcgat cttgatcatg tcgaaggtgt catagtgcat gccaatgatg
     1261 cgatcgcagc ggataaagtc cgacgcgatg acggcattgt cgatgcccat ggtgaagttg
     1321 ttcccgatcg gcaggaaggc gagatccatc ttcttgtaat caccaatcag tttcatgtcg
     1381 aggtgcagcg cggtgtctcc ggcataat
//
LOCUS       DAOTON010000805         1406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_73484_length_1406_cov_2.538860, whole genome shotgun sequence.
ACCESSION   DAOTON010000805 DAOTON010000000
VERSION     DAOTON010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73484_length_1406_cov_2.538860"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1406)
                     /locus_tag="P5292_12930"
     CDS             complement(<1..>1406)
                     /locus_tag="P5292_12930"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS40077.1"
                     /translation="ICPAGDCGALYDPGGFQNAVETKKRAQSPVIDCSSQNNVVLTFL
                     YIENGDGATDNALVEYYDGTTWSVIGDMPKSTSCGGYSTWTRYTVALPTSADFNPNVR
                     IGFTWVNDNGGVGLNPSFAVDSIALAGVPAPVADFEASDTLICVGDCIDFYDQSLNFP
                     DAFTWNFFGGTPATSSSQNPTSVCFNAPGTYTIQLIVGNAAGSDTLTLANYIRVEPCV
                     GPTADFSVSDSSVCERSCVDFTDQSVNGALSWLWLFPGGTPSTSTDPSPTGICYNTPG
                     LYDVTLIVSNAFGADTLTKSGYMNIETCPLPVANFATFTPAICSNQCVDFFDQSQFTD
                     TSTQWFWYFPGATPDTSTLRNPTNICYPQDGLYDVQLIVTNSYGSDTTLKYSFIQVES
                     VPTAFVGPDTSMYYGSSYQLTAGGGVTYQWSPAAGLDSVLALFPALSDKLGRTAGTLS
                     GGEQQMVAIGRALMSGPR"
BASE COUNT          319 a          363 c          456 g          268 t
ORIGIN      
        1 gcgcgggccg gacatcagcg cccgcccgat ggcgaccatc tgctgctcgc cgccggagag
       61 ggtgccggcg gtgcgcccga gcttgtccga cagcgcgggg aacagcgcga ggacgctgtc
      121 cagccccgcc gccggtgacc attggtaggt caccccgccg ccggcggtca gctggtagga
      181 actgccgtaa tacatggagg tatcgggccc gacaaaggct gtggggacgc tctcaacctg
      241 gatgaacgaa tattttaagg tggtatcgga gccgtaggaa ttcgttacga tcaattgtac
      301 gtcatacagt ccgtcctgcg ggtaacagat gttggtcggg ttgcggagcg tactggtgtc
      361 gggagtggcg ccggggaaat accaaaacca ctgcgtggaa gtatcggtga actgcgactg
      421 atcaaagaag tcgacgcact gattcgaaca aatcgccggc gtgaaggtgg cgaaattggc
      481 gaccggtaac ggacaggttt caatgttcat gtaaccggac ttcgtgagcg tatcggctcc
      541 gaaggcgttc gagacgatga gcgtgacatc gtacaaaccc ggagtattgt agcaaatgcc
      601 ggtagggctg gggtccgtgg aagtggaggg tgttccgccg gggaacagcc acaaccaact
      661 gagcgcgccg ttcacactct gatcggtgaa gtcaacgcaa ctgcgttcac agacggaaga
      721 gtcgctgacc gagaaatcgg ctgtaggtcc gacgcagggt tcaacacgga tgtagttggc
      781 aagcgtaagg gtgtcgctgc cggcagcgtt gccaacgatc aattggatcg tataggtgcc
      841 gggcgcattg aaacagacac tcgtcggatt ctgagaagat gaagttgcgg gagtgccgcc
      901 aaagaagttc caggtgaaag catccggaaa gttgaggctc tgatcgtaga aatcaatgca
      961 atcgcctacg cagatcaacg tatcgctggc ttcaaagtcg gcgaccgggg ccgggacacc
     1021 ggccagtgcg atgctgtcga ctgcgaacga agggttgagg ccgactccgc cgttgtcatt
     1081 cacccaggtg aaacctatac gaacgttcgg gttgaaatcg gcagaagtag gaagtgcgac
     1141 cgtgtaacgc gtccaggtgg aataaccgcc acaactcgtg gacttgggca tgtcgccgat
     1201 gacggaccag gtggtgccgt cgtagtattc aaccagcgcg ttgtccgtag cgccatcgcc
     1261 gttctcgatg tagaggaacg tcagcacgac gttgttctgg ctgctgcaat cgatgaccgg
     1321 cgactgggcg cgttttttcg tttcaacagc attctgaaat ccacccggat cgtagagtgc
     1381 gccgcaatca cccgcgggac aaatga
//
LOCUS       DAOTON010000806         1405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_73583_length_1405_cov_2.225926, whole genome shotgun sequence.
ACCESSION   DAOTON010000806 DAOTON010000000
VERSION     DAOTON010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73583_length_1405_cov_2.225926"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="P5292_12935"
     CDS             complement(<1..1017)
                     /locus_tag="P5292_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40078.1"
                     /translation="MRQNLTRISAFLLLLLTVFASSQRASAQGDAGVIDITAPSASLC
                     AGANDVKAVVTNYGAGPITLMTIGWSVNGVAQTPFNYTNTLLPGDNDTVTVGSFNFPG
                     GSTFTIDAFTADPNGLPDTDASNDSLSKSGFTSALSGTYTIGGTTPDFARFTDAVTAL
                     TTYGVCGPVTFNIRPGVDTMQTVIPEIAGADSTNNIRFQSESGDSSSVVLTYPTQPSF
                     TPTNYLIQLDGADYLTFHQLTLQRTGIEPYGRILEFRNTATHITVSNCHLIGSTTTAV
                     TNSLAALVYSSAGTSSNDSSNVFTNSRFERGTLGIYMNGINTLQLEQDIHITNNTFVD
                     QYSKG"
     gene            complement(1159..>1405)
                     /locus_tag="P5292_12940"
     CDS             complement(1159..>1405)
                     /locus_tag="P5292_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40079.1"
                     /translation="ILPIDQRISWESHLLGSVAGIIAALYFRKDGPQKPKPQWQLDEE
                     QGLTPEAEAEQVAAEQQPDRPTFRYDYRPSDETEERD"
BASE COUNT          274 a          356 c          462 g          313 t
ORIGIN      
        1 acccttcgaa tactggtcaa cgaaggtgtt gttcgtgatg tggatgtcct gctctagctg
       61 aagggtattg ataccgttca tgtaaatacc cagcgtgcct cgctcgaagc ggctgttggt
      121 aaagacgttc gaggagtcgt tggaggaagt tcctgccgag gagtaaacaa gcgccgcgag
      181 tgaattcgtt accgccgtgg tggtcgaacc gatcaggtga cagttcgaga ccgtgatgtg
      241 cgtagcggtg ttgcggaatt cgaggatgcg gccgtacggc tcaatgccgg tacgctgaag
      301 cgtgagctgg tggaaggtca ggtagtcagc gccatccagt tggatcaggt agttggtcgg
      361 ggtgaaggac ggctgtgtcg ggtaggtcag taccacggag gagctgtcac ccgattcgct
      421 ctggaagcgg atgttgttcg tcgagtcagc gccggcgatc tccggaatca cggtctgcat
      481 cgtgtccaca cccggacgga tgttgaacgt caccgggcca caaacgccgt aagtcgtcag
      541 cgccgtcacc gcgtcggtga agcgtgcgaa gtcgggtgtc gtaccgccga tggtgtaggt
      601 tcccgagagg gcggaggtga agccgctctt cgacaaggag tcgttcgagg cgtcggtgtc
      661 cggcaaaccg ttcggatcgg ccgtaaaggc atcgatcgtg aaggtcgagc cgccggggaa
      721 gttgaacgag cctacggtga ccgtgtcgtt gtcgcccggc agcagggtgt tggtgtagtt
      781 gaacggagtc tgcgcaacgc cattcacact ccagccgatc gtcatcaggg tgatcggtcc
      841 ggcgccgtag ttcgtcacga cggctttcac atcattcgct cccgcgcaaa ggctggctga
      901 gggcgctgtg atgtcgatca cgcccgcgtc accttgcgcg gatgcccgct gcgatgatgc
      961 gaacaccgtc aggagcaaca gcaggaaggc tgaaatcctt gtcaagtttt gtctcatgga
     1021 ttggattatt gtagggttga aatttgattc ttgtgagcta accgggtaaa ggtaccccgc
     1081 accgttcgga ggccgggtta aaacaggtgc aatctttcga caaaaagtgt gttgataacc
     1141 ccggattttc agagggattt aatcccgttc ctccgtctca tcggacgggc ggtagtcgta
     1201 acggaaagta gggcgatcag gttgctgttc cgctgcaact tgttccgcct cggcttccgg
     1261 tgtcaagccc tgttcttcgt ccagttgcca ttggggtttg ggcttctgcg gaccgtcctt
     1321 ccgaaagtac agcgcggcga tgatgccggc caccgagcca agcaggtgcg actcccagga
     1381 aatgcgctgg tcgatcggca ggatg
//
LOCUS       DAOTON010000807         1402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_73790_length_1402_cov_3.042316, whole genome shotgun sequence.
ACCESSION   DAOTON010000807 DAOTON010000000
VERSION     DAOTON010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73790_length_1402_cov_3.042316"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="P5292_12945"
     CDS             complement(<1..1251)
                     /locus_tag="P5292_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS40080.1"
                     /translation="MIHVNNLSLRYGKRVLFEEVNLKFTPGNCYGVIGANGAGKSTFL
                     KILSGEIDPTTGQVHITPGERMSVLKQDHFAFDQCQVLQTVLMGNKKLWDVMQEKDAL
                     YAKADFTDADGERAAVLEQQFADLHGWDAESDAATLLSSLGIREELHQQWMSELSGKE
                     KVRVLLAQALFGNPDILLLDEPTNDLDVDTITWLENFLADFQNTVIVVSHDRHFLDAV
                     CTHVADIDFGKINLHSGNYTFWYESSQLALRQRADQNKKIEDKRKELQEFIERFSANA
                     SKSRQATSRKKLLEKLVVDDIQPSNRKYPGIIFTAERDCGDQILNVDGLTKIDHEGKP
                     LFRDISFTLRKGDKVAFLARDHQSVTALLRTLMNDEAADSGEFKWGTTITCSYLPNDN
                     TAFFQSDLNLVDWLRQYSKDKDETY"
BASE COUNT          273 a          365 c          414 g          350 t
ORIGIN      
        1 ataggtttca tccttgtctt tggaatactg ccggagccag tcgaccaggt tgaggtcgct
       61 ctggaaaaac gcggtattgt cgttcggcag ataagagcag gtgatggtgg ttccccattt
      121 gaattctccg ctgtcggcag cttcgtcgtt catcagcgta cgcagcaggg cggtgacaga
      181 ctggtggtcg cgcgcgagga acgcgacttt gtcgccctta cgaagcgtga agctgatatc
      241 gcggaagagc ggcttacctt cgtggtcgat cttggtcaat ccgtcgacgt taaggatctg
      301 gtcaccgcag tcgcgctcag cggtgaagat gatgccggga tacttgcggt tggaaggctg
      361 gatatcgtcg accaccagct tctccaacag tttcttacgg ctggtcgcct gacgtgactt
      421 cgaagcgttg gcactgaagc gttcgatgaa ctcctggagt tccttgcgct tgtcttcgat
      481 cttcttgttc tggtcggcgc gctgacgcag ggcaagctgg ctcgattcgt accagaacgt
      541 gtagttgccg ctgtgcaggt tgatctttcc gaaatcgata tccgcaacgt gcgtacacac
      601 cgcgtcgagg aagtgacgat cgtggctcac gacgatcacg gtattttgga aatcggcgag
      661 aaagttttcc agccaggtga tggtgtcaac gtcgaggtcg ttcgtcggtt cgtcgagcag
      721 caggatatca ggatttccga acagtgcctg tgccagcagt acccgtactt tttctttacc
      781 actcagttcg ctcatccatt gctggtggag ttcttcccga atacccaggc tcgaaagcag
      841 ggtcgctgca tcgctctcgg catcccagcc gtggaggtcg gcgaattgct gttcgagcac
      901 ggcggcccgt tcaccgtcgg catcggtgaa gtcggccttt gcatagaggg cgtccttctc
      961 ctgcatcaca tcccacaact tcttgttacc catgagtacg gtctgcagca cctggcactg
     1021 atcgaaagcg aagtggtcct gcttgagtac gctcatgcgc tcgccgggag tgatgtgtac
     1081 ctggccggtg gtcgggtcga tctcaccgga aaggatcttc agaaaagtgg attttccggc
     1141 tccattcgcg ccaatgacgc cataacagtt gccaggcgtg aacttgagat tgacctcctc
     1201 gaacagcacg cgtttgccgt agcggaggga aaggttattt acgtggatca tgcgggcggc
     1261 aaaggtacga tttcggaagc gtttttcctt gagaatcagt gggtgaagcg agagcccgga
     1321 ccgtcggcac actgacgggt taatatttta cctgtttatc cgaatcccgt atctgtggct
     1381 gcgggtgccc gaccatacat tt
//
LOCUS       DAOTON010000808         1400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_73977_length_1400_cov_2.979926, whole genome shotgun sequence.
ACCESSION   DAOTON010000808 DAOTON010000000
VERSION     DAOTON010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73977_length_1400_cov_2.979926"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /gene="xseA"
                     /locus_tag="P5292_12950"
     CDS             complement(<1..867)
                     /gene="xseA"
                     /locus_tag="P5292_12950"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRS40081.1"
                     /translation="MSGPNNYLRLSDLTREIRDVVQQHFTGRTYWIVAEISGHKYYPN
                     TGRHYLSFVEKIGEGGAETAKIKAKAWSQGSDRIRAFEQSTGQSFTDGIQVLVQVSVE
                     YHLVHDLSLTILDVDPTFTLGVLERQRRETLDRLVRDNPGYVEKVGEEYVTYNKRLAL
                     RPVVQRIALVASPKSAGYEDFLHTLHENEAGYRFAIDYYYTSVQGAEAEDELVRALID
                     VYQSKKAYDAVVIIRGGGAKTDFLVFDTYRVSQAVARFPVPVITGIGHHQDVSITDRM
                     AHTQTKTPTQAAE"
     gene            complement(904..>1400)
                     /locus_tag="P5292_12955"
     CDS             complement(904..>1400)
                     /locus_tag="P5292_12955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40082.1"
                     /translation="NRLEWVTATETNNDYFSLERSRDGLEFSTIARVDGAGNSSQPLH
                     YQYLDTEAGDGLWYYRLRQNDFDGNFSYSDPVALRRGKLKDRFSVYPNPTAGRFTIGL
                     TTNTYAEYEVNVFDALGEVVFSSFETARAAKEISLEHLSAGVYTLLLRCDGEQAAFKL
                     VIER"
BASE COUNT          290 a          421 c          390 g          299 t
ORIGIN      
        1 ctcggcagct tgggtcggag tcttggtctg tgtatgcgcc atccggtcgg tgatgctcac
       61 atcctggtga tgcccgatgc ccgtgatcac cggaaccggg aaccgtgcca ccgcctgact
      121 gacgcgatag gtgtcgaaca ccaggaagtc ggtcttcgcg cctccgcccc ggatgatgac
      181 cacggcatcg tacgctttct tcgactgata gacgtcgatc agggcacgca ccagttcatc
      241 ctccgcctcc gcgccctgca cgctggtgta atagtagtcg atcgcgaaac gatatcccgc
      301 ttcgttctcg tgcagggtat gcagaaaatc ctcgtaaccc gccgacttcg gtgaagcgac
      361 cagcgcgatg cgttgcacca ccggtcgcaa tgccaggcgc ttgttgtagg tgacatactc
      421 ctcccccact ttttccacat agccgggatt gtcgcgtacc agccgatcga gtgtttctct
      481 ccgctgacgc tccaacaccc ccagggtgaa ggtcggatcc acatcaagga tcgtaagtga
      541 aaggtcgtgt accaggtgat actccacgga aacctgcacc agcacctgga tgccgtcggt
      601 gaacgattgt ccggtcgatt gttcgaaagc ccggatgcga tccgacccct ggctccaggc
      661 cttcgccttg atcttggccg tctccgctcc gccttctccg atcttctcga cgaagcttag
      721 gtaatggcgg ccggtattcg gatagtactt atgcccggaa atttccgcta cgatccaata
      781 cgtgcgaccg gtgaaatgct gctgcaccac atcgcggatc tcgcgtgtga ggtcggacag
      841 acgtaaataa ttgttcggac cggacatgcc cgacgaaggt acacaaaacg aaaagggaaa
      901 gactcagcgt tcgatcacca gcttaaatgc ggcctgctcg ccgtcgcagc gcaacagcag
      961 cgtatagacg cctgccgaca ggtgctcgag agaaatttcc ttcgcggcac gtgcggtttc
     1021 aaatgagctg aacaccactt caccgagcgc gtcgaacacg ttcacttcat actcggcgta
     1081 cgtattcgtc gtcagcccga tggtgaagcg accggctgtc gggttgggat aaacggaaaa
     1141 gcggtctttc aacttaccac gacgcaacgc caccggatcg ctgtaggaaa agttgccatc
     1201 aaagtcgttc tggcgcaagc ggtagtacca cagtccgtca ccggcctcgg tatcgaggta
     1261 ttggtaatgc aggggctggc tcgaattgcc ggctccgtcg acgcgcgcga tcgttgaaaa
     1321 ctccaggccg tcgcggctac gctccaggga gaagtagtcg ttgttggttt ccgtggccgt
     1381 tacccattcc aggcgattac
//
LOCUS       DAOTON010000809         1398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_74129_length_1398_cov_2.993299, whole genome shotgun sequence.
ACCESSION   DAOTON010000809 DAOTON010000000
VERSION     DAOTON010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74129_length_1398_cov_2.993299"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>856)
                     /gene="priA"
                     /locus_tag="P5292_12960"
     CDS             complement(<1..>856)
                     /gene="priA"
                     /locus_tag="P5292_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRS40083.1"
                     /translation="IFLPFSRLGLVIVDEEHDPSYKQQDPAPRYNGRDAAIVLAAGHG
                     GKVLLGSATPSLESYTNARQGRYGLINLHERFGGIRMPEISVADIADASRKKLMKAHF
                     TPELLDAVRSALAKEEQVILFQNRRGFSSFLECRHCNWIPHCKNCSVTLTYHKQQHQI
                     KCHYCGYAQSVPSACPNCGDHHIEVRGFGTERIEEEIAVFFPDHRIARFDLDTVRTKS
                     SLHRLLHDFSERRIDLLVGTQMVSKGLDFDNVSTVGILDADQMLNFPDFRSFERSFQL
                     MSQVAGRSG"
     assembly_gap    857..866
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<867..>1398)
                     /locus_tag="P5292_12965"
     CDS             complement(<867..>1398)
                     /locus_tag="P5292_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRS40084.1"
                     /translation="KLRLDQAGTDYVRKSALTAHEQVSGSALQTLIRNGILEEYAAVI
                     DRVPAFSGNVQPVAELSENQQRALQQIQAALLEHPVALLHGVTSSGKTELYIHLIDEQ
                     LRAGRQVLYLLPEIALTTQLVQRLQRYFGAAVSVYHSRFNSNERVEVWKQVLHFDQTD
                     QQTPFPRKGQLVLGARS"
BASE COUNT          318 a          396 c          374 g          300 t
ORIGIN      
        1 cccgaccgac ctgcaacctg cgacatcagc tgaaagctgc gctcaaaaga cctgaagtcc
       61 gggaagttga gcatctggtc ggcatccaga atccctacgg tactgacatt gtcgaagtcc
      121 agacccttcg agaccatctg tgtcccgaca agcaggtcta tgcggcgctc tgagaaatca
      181 tgcaacagcc gatgcaggga cgatttggtt cggacagtat ccaggtcgaa gcgagcgatg
      241 cggtgatccg gaaagaacac ggcaatctcc tcctcgatcc gctccgttcc gaagcctctg
      301 acttcaatat gatgatcgcc gcagttcgga caagccgaag gcaccgactg ggcatatccg
      361 caatagtgac atttgatctg gtgttgctgc ttgtgatagg tcagcgtgac cgaacagttc
      421 ttgcaatgcg ggatccagtt acaatgacgg cattccagaa acgatgaaaa gccgcgcctg
      481 ttttgaaaaa ggatcacttg ttcttccttc gctaacgcgc tgcgcacggc atcgagtaat
      541 tccggcgtga agtgggcctt catcagtttc ttccgggaag cgtcagcgat atccgcaaca
      601 ctgatctccg gcatacgaat acccccgaaa cgctcgtgca ggttgatcaa tccatagcga
      661 ccctgccgcg cattggtgta gctctccagg gaaggcgtgg cagaacccaa caacaccttg
      721 ccgccatgac ctgcagcgag gacaatcgcc gcgtcccgtc cgttatagcg tggcgcaggg
      781 tcttgttgct tataggaagg atcgtgctct tcatcgacga tgactagtcc taaacgggaa
      841 aacggaagga aaatggnnnn nnnnnncgat cgcgccccca ggaccaattg cccctttcgc
      901 ggaaacggag tttgctggtc cgtctgatcg aaatgcagga cctgcttcca aacctctacc
      961 cgttcattgc tgttgaaccg agagtggtaa acggatacgg ctgctccgaa atagcgctgc
     1021 aagcgttgta ccagttgcgt cgttaacgcg atctccggaa ggagatacaa tacctgacga
     1081 ccagcccgga gctgttcatc gatcaggtgg atgtacaatt ccgtcttccc gctcgacgtt
     1141 acaccatgca gcaaggccac cggatgctcc agcaaggccg cttgtatctg ttgcaaagcg
     1201 cgttgttggt tctcactcaa ctccgccacg ggctggacat tgccggaaaa tgcagggacg
     1261 cggtcgatca ccgctgcgta ctcttccagg atcccgtttc ggatcagggt ttgaagcgcg
     1321 gaaccggaga cctgctcatg cgccgttaag gctgatttcc ggacatagtc ggtcccggct
     1381 tgatccagcc tcagtttc
//
LOCUS       DAOTON010000810         1396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_74282_length_1396_cov_4.049217, whole genome shotgun sequence.
ACCESSION   DAOTON010000810 DAOTON010000000
VERSION     DAOTON010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74282_length_1396_cov_4.049217"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..749)
                     /locus_tag="P5292_12970"
     CDS             complement(57..749)
                     /locus_tag="P5292_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRS40085.1"
                     /translation="MIRCIALDDEPPALTVLQRYAERVDILQLDGTFTRPSEAQQYLK
                     DQAVDLLFLDIQMPSISGIDFYTSLNKPIPVIFTTAFADYAVQGFNLNAVDYLLKPYS
                     FERFQQAVRKFSERQQRGSTPAAAEEPFLLIKADYRLVKVKLKDIEFIEGLDDYLKIH
                     LLEQKTIVTRMTMKSMIEKLTAEQFIRVHRSFIVPLSRIESVRSKMIRIGKEDIPIGN
                     SYEEGFFRVFKG"
     gene            complement(746..>1396)
                     /locus_tag="P5292_12975"
     CDS             complement(746..>1396)
                     /locus_tag="P5292_12975"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRS40086.1"
                     /translation="VFLAVLFIALSLRLIQQWRRSERERLHAELSFLKAQVHPHFLFN
                     TLNSIYSLALSRSDETAPAIVKLSEMMRYVITEASEEFVPLEKELHYIRDYIDLQSLR
                     LDKNAKVHSSIEGDPDNLLIAPLLLIPLIENAYKYGVNPEDDSVIDIRIRVSGTRVTL
                     DIANTKVDANHNKNIALGIGLHNTRQRLELFYPGRHTLTIDETETEYRLHLSVQLA"
BASE COUNT          309 a          355 c          404 g          328 t
ORIGIN      
        1 ctcggtctcg gtcacgctcc tcgtccctgc cttttaaaga ccggaaactc gaaaccctaa
       61 cccttgaaga cccgaaaaaa cccttcctca tagctgttcc cgatcgggat gtcctctttg
      121 ccgatccgga tcatcttgct gcgaacgctt tcgatgcgcg acaagggtac gatgaacgaa
      181 cggtgaacac ggatgaattg ttccgcagtc agcttttcga tcatcgactt catggtcatc
      241 cgggtgacga tcgtcttctg ctccagcaaa tggatcttca ggtaatcgtc gaggccttcg
      301 atgaactcga tgtccttcaa tttgactttc accaggcggt aatcggcctt gatgagcaga
      361 aaaggttcct cagccgccgc cggtgtgctt ccccgttgct ggcgttcgga aaatttccgg
      421 acggcctgct ggaagcgctc gaaggaatag ggcttgagca ggtagtcgac cgcattcaga
      481 ttgaatccct gtaccgcgta atccgcaaaa gcggtcgtga agatcaccgg aatcggtttg
      541 ttgagggaag tataaaagtc gatacctgaa atggacggca tctgaatgtc gagaaacaac
      601 aagtcgaccg cttggtcttt caggtactgc tgcgcttccg atggacgcgt gaaggtacca
      661 tcgagctgta ggatatccac acgctccgca tagcgctgca ggacggtcag tgcgggcggt
      721 tcatcatcaa gtgcgatgca gcggatcatg ccagttgaac ggataagtgt aaacgatatt
      781 cggtttccgt ttcgtcgatc gtgagcgtat gccggcccgg ataaaaaagt tccagccgtt
      841 ggcgcgtgtt gtgcaagccg atgcccagcg cgatgttctt gttgtggttg gcgtcgacct
      901 tggtgttggc gatgtcgagc gtgacgcgtg ttccggaaac gcgaatgcgg atgtcgatca
      961 ccgaatcgtc ttcgggattg accccgtact tgtacgcgtt ctcgatcagc gggatgagca
     1021 ggagcggagc gatcaggaga ttgtcgggat cgccttcgat cgacgaatgg accttcgcgt
     1081 tcttgtcgag gcgaaggctt tgcaggtcga tgtagtcgcg gatgtaatgc agttcctttt
     1141 cgagcgggac gaactcttcc gaagcttcgg tgatcacata gcgcatcatt tccgacaatt
     1201 tcacgatcgc aggagcggtc tcgtcagagc gcgaaagcgc cagtgaatag atgctattca
     1261 aggtgttgaa gagaaaatgc ggatggacct gcgctttcag gaaactcagt tcagcatgca
     1321 ggcgttcgcg ttccgagcgt cgccactgct ggatcagccg caggctgagc gcgatgaaca
     1381 gcacggcaag aaatac
//
LOCUS       DAOTON010000811         1396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_74292_length_1396_cov_3.321402, whole genome shotgun sequence.
ACCESSION   DAOTON010000811 DAOTON010000000
VERSION     DAOTON010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74292_length_1396_cov_3.321402"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..577)
                     /locus_tag="P5292_12980"
     CDS             complement(116..577)
                     /locus_tag="P5292_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HRS40087.1"
                     /translation="MKRIITLLLLVVTTLAVSAQKQSEPKSFGEKITADNAMEASKLP
                     ELLKGKENVQAKITGTVNEVCQKKGCWAKMDVGNGETMMIRFKDYGFFLPKDCAGKKL
                     VLNGIATYEVTTVEELRHYAQDAGKSKEEIEKITEEEKSLSFEADGVLLYE"
     gene            655..>1396
                     /locus_tag="P5292_12985"
     CDS             655..>1396
                     /locus_tag="P5292_12985"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRS40088.1"
                     /translation="MIKRLAPWIFSALFAACASKKEANNEKTFMTTQDPHTFAQPDSA
                     RMTHLSLDLTVDFKRQVLEGTATCSVDVQPGSTSVIFDTRDLHIASVAVDGQPVQDFQ
                     LGDSVEYLGQALRVPVTTGKHSVSIRYSTSPQASALQWLSPQQTAGKKYPFLFTQSQA
                     ILARTWAPVQDSPGIRFTYDATLHVPDGLMAVMSAENPTARSKDGVYRFKMEQAIPAY
                     LLALAVGDFDYHRYDDRSGVYAEPATLPM"
BASE COUNT          281 a          433 c          354 g          328 t
ORIGIN      
        1 gagcagggaa agctcgctcg atccgttcgg attgcgatcc tgcaccggtt cctgcggcgt
       61 catccaggac gacagcaaga cgaccgatgc gaggacgagg gaagagatcc agtacttatt
      121 catagagcaa tacgccgtca gcttcgaacg aaagtgattt ctcctcttcc gtgatctttt
      181 cgatttcttc cttcgactta cccgcgtcct gggcatagtg gcgaagttct tccaccgtcg
      241 tgacctcgta ggtagcgatg ccgttcaaca ccagtttctt gcccgcgcaa tccttcggca
      301 ggaagaagcc gtagtctttg aaacggatca tcatcgtttc gccattgccg acgtccatct
      361 tcgcccagca acctttcttc tggcagactt cgttgaccgt tccggtgatt ttcgcctgga
      421 cgttttcttt tcctttcagg agctccggca gcttggatgc ctccatggca ttgtcggccg
      481 tgattttttc gccgaacgac ttcggttcgg actgtttctg tgcgctgacc gccaaagtcg
      541 tgacgaccag caggagcagg gtgatgattc gtttcatgat cggtttttcg ttaaacaaag
      601 ttagccaatt tacccggaac gggccgcctt gcgccggatt gctattttta cggcatgatc
      661 aaacgactgg ccccctggat cttttctgcg ctttttgcag cctgcgcttc caaaaaagag
      721 gccaacaatg aaaaaacgtt catgactact caagatccgc acaccttcgc acaacccgat
      781 tccgcccgga tgacccacct gagcctcgac ctcacggtcg acttcaaacg ccaggtcctg
      841 gagggtaccg ctacctgctc cgtggacgtc caaccgggca gtaccagtgt catcttcgat
      901 acgcgcgacc ttcacattgc atcggtcgcc gttgacggac aaccggttca ggatttccag
      961 ctcggcgatt ccgtcgaata cctcggacaa gccttgcgcg taccggtaac caccggtaag
     1021 cattccgtct cgatccgcta ttcgacatcc ccacaagctt cagcgctgca gtggttgagt
     1081 ccgcagcaga ccgccggaaa aaagtaccct ttcctgttta cccagtcgca ggcgatcctc
     1141 gctcgcacct gggcgccggt acaggatagt ccgggcatcc gtttcaccta cgacgcgacc
     1201 ctgcatgtac ccgacgggct gatggccgtg atgagcgccg aaaacccgac tgcccgatcg
     1261 aaagacggtg tgtaccgatt caagatggaa caggcgatcc cagcttacct gctcgcgctg
     1321 gcggtcggtg atttcgacta ccaccgctac gacgatcgtt ccggcgttta cgccgagccc
     1381 gccacccttc cgatgg
//
LOCUS       DAOTON010000812         1395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_74372_length_1395_cov_3.629104, whole genome shotgun sequence.
ACCESSION   DAOTON010000812 DAOTON010000000
VERSION     DAOTON010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74372_length_1395_cov_3.629104"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..745)
                     /locus_tag="P5292_12990"
     CDS             complement(104..745)
                     /locus_tag="P5292_12990"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRS40089.1"
                     /translation="MARYCQELGYEYLGICDHSRSAFYANGLSIERVAEQHKEIEALN
                     KKLAPFRILKGIESDILTDGSLDYPDEILDTFDLVVASVHSVLKMDENKATARLIRAV
                     EHPATTILGHPTGRLLLSRPGYPIDHRKVIDACAANDVIIELNANPYRLDIDWRWIPY
                     ALDKGVMISINPDAHRKEGYHDMHFGVRAARKGGLTKEMTFNALSLVELEKRL"
     gene            1283..1395
                     /locus_tag="P5292_12995"
                     /pseudo
     CDS             1283..1395
                     /locus_tag="P5292_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
BASE COUNT          243 a          363 c          462 g          327 t
ORIGIN      
        1 agggacgagg gaacggtaat ccctcaaggt agcgattttt ctattcgctg ttcgctattc
       61 actatttact gtttactgtt tactgttcac tgttcactcc cttctacagc cgcttctcca
      121 actccaccag tgacagcgca ttaaacgtca tctctttcgt gagtcctcct tttcttgctg
      181 cgcggacgcc gaagtgcatg tcgtggtagc cttctttgcg gtgggcgtcg gggttgatgg
      241 agatcatgac gcctttgtcg agtgcatagg ggatccagcg ccagtcgatg tcgaggcgat
      301 agggattcgc gttgagttcg atgatcacgt cgttggcggc gcaggcatcg atcactttgc
      361 ggtggtcgat cgggtagccg gggcgggaga ggagaaggcg cccggtcgga tggccgagga
      421 tggtggttgc cgggtgttcg acggcgcgga tgaggcgtgc ggtggcttta ttttcgtcca
      481 tcttcaggac cgagtggaca gaagcgacga ccaggtcgaa ggtgtcgagg atttcatccg
      541 ggtagtcgag cgagccgtcg gtgaggatgt cggactcgat ccctttcagg atccggaacg
      601 gggcgagttt cttgttgagc gcttcgatct ctttgtgctg ctcagccacg cgttcgatcg
      661 aaaggccgtt tgcataaaat gcgctgcggc tgtggtcgca gatgccgagg tattcatagc
      721 cgagctcctg gcaatagcgt gccatgactt cgagcgaatg cgtgccgtcg gaccaggtgg
      781 agtggttgtg caggatgccg cgcaggtcgg cttccgtgat gagctttgac gtatcgggtc
      841 gctggcgtgt ttcccatagc tcctcgcgta gctcaggttc gggaaacgcg taaccgatcg
      901 tacgatagat ctccacctct gaaccttcga gtgaggcgtt ccagttgaac gcgctgctga
      961 gacgcgccag gtgcgcttca tcgcctgtcg tgcgaagcag ggtgttgccg aaggcttttt
     1021 cggaacagca atgaaacacg agcggcagtc ttccggccag tgagctgcgg accatcgttc
     1081 catcggtgga aacaggttgt cctgccacca ggggatggtc ggcgagaaaa gcggtgatct
     1141 tttcagttgt ggctactgtt acgaattccg ccgtttcgat gatctcgcaa ccgcggcgaa
     1201 gtgtgccggt ccagtcggcg gagccgatac ccgaagtttg ccgcaagtct gcgagcagtt
     1261 gaagcgcaag gggttcgatg accgcacgct gatcgccttc ggcggcggcg ggccggtgca
     1321 tgggctgcgc gtggccgaga aatgcggcat cacccgcatc gtcgtgccga ccggcgccgg
     1381 cgtgggcagc gccat
//
LOCUS       DAOTON010000813         1389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_74861_length_1389_cov_3.886807, whole genome shotgun sequence.
ACCESSION   DAOTON010000813 DAOTON010000000
VERSION     DAOTON010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74861_length_1389_cov_3.886807"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="P5292_13000"
     CDS             complement(<1..429)
                     /locus_tag="P5292_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40090.1"
                     /translation="MHQQQSFRLKQKTRRPLGSAGFFFCFLLLSLVASQKSLHAQPAW
                     VDTLRYSLHQRPKFFFQLTSFNSFVSSDFANFSGFRMGLNYNHRLRFGMGLLTLDGNA
                     VVSAVSIREGDISYITNGQLKTTFLTFSGEYIFYSKYPWQF"
     gene            complement(448..702)
                     /locus_tag="P5292_13005"
     CDS             complement(448..702)
                     /locus_tag="P5292_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRS40091.1"
                     /translation="MKLFVKNIEESVNEALLEAIFKQFGEIVSTKIVYDKITWESRGF
                     GFVEFKNSQDAMKAMDELQGKELVGKKLIIMEALEKGAGG"
     gene            complement(732..1208)
                     /locus_tag="P5292_13010"
     CDS             complement(732..1208)
                     /locus_tag="P5292_13010"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HRS40092.1"
                     /translation="MKIMLPFLVLIGFLVACDKKDDDSSTTNPPAATGCTSLLPNKLS
                     AKIANQEWCSDQSCFGDLAVVMTINGLNGNGSSLTLELDSVMPGTYQITEDVNHMIYL
                     NGDGWESTNDNPGTLVITSNNTSTNQIKGTFTVTLRSPLSGNLSVTEGKFDLFYTE"
BASE COUNT          340 a          354 c          343 g          352 t
ORIGIN      
        1 gaattgccag ggatacttcg aatagaaaat atactctcca ctgaaggtca ggaacgtggt
       61 cttcagttga ccgttggtga tgtaggagat gtccccctcc cggatggaaa ccgcgctcac
      121 cacggcgttg ccgtcgaggg tcaacagacc catcccgaag cgcaggcgat gattatagtt
      181 caaccccatc cgaaaaccgg aaaagttggc aaagtcgcta ctgacaaaac tattgaagga
      241 ggtaagttgg aaaaagaatt tggggcgctg atgcaaactg taacggagcg tatcgaccca
      301 tgccggctgc gcgtgcagac ttttctgaga agcgaccaac gagagcaaca ggaaacaaaa
      361 gaagaaaccc gcagacccca gtggtcgacg ggttttttgt ttcaagcgga agctttgctg
      421 ttggtgcatg cgaggatgcg tcgtcgatca accgccagct cccttttcca gcgcttccat
      481 gatgatcagt tttttcccga ccagttcctt gccttgcaat tcatccatcg ccttcatggc
      541 atcctgacta ttcttgaatt ccacaaatcc gaaaccgcgg gattcccagg tgatcttatc
      601 gtacacgatc ttggtgctga cgatctcgcc gaattgcttg aagatggctt ccagtaacgc
      661 ttcgtttacg gactcctcga tgtttttgac gaataacttc attgccctaa aggtaccaat
      721 aatccaatgg tttattccgt gtaaaagaga tcaaatttgc cttcggtcac cgacaggtta
      781 cccgaaagcg ggctccgcaa ggtcaccgtg aaggtgcctt tgatctggtt cgtcgacgta
      841 ttgttggagg tgatgacgag cgtgcccgga ttatcgttgg tcgattccca accgtcgcca
      901 ttgaggtaga tcatgtgatt gacatcctcc gtgatctgat aggttcccgg catgacgctg
      961 tccagttcca acgtcaggga tgaaccatta ccattcaacc cattgatcgt catgacaacg
     1021 gcgagatctc cgaaacaact ctgatcggag caccattcct gattggctat tttcgcggaa
     1081 agtttgttgg gaagaaggct cgtacaaccg gtagcagccg gcggattggt tgttgatgaa
     1141 tcgtcgtctt tcttgtcaca cgcgaccagg aatccgatca acacgaggaa ggggagcatt
     1201 attttcatag gttgcgaaat taataaggga ttgttttcgt ttaaagcctt accatgaatc
     1261 ataccacttc taccgtcttc caaacggatt tcgcctttac catgcaccgt cagctcagcc
     1321 tgctggcgga tgaaatccgc gcgtatcctt ctgataccgc cctatggatc gtcccggccg
     1381 gtattacca
//
LOCUS       DAOTON010000814         1384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_75346_length_1384_cov_2.276900, whole genome shotgun sequence.
ACCESSION   DAOTON010000814 DAOTON010000000
VERSION     DAOTON010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75346_length_1384_cov_2.276900"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="P5292_13015"
     CDS             complement(<1..421)
                     /locus_tag="P5292_13015"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRS40093.1"
                     /translation="MVNIRLLDIQVAQMLQSRLLPDLYYHSPAHTRDVLRSALRIGRE
                     EGLSPFELELLGAAAILHDTGFTVAYADHEWHSCAIARILLPQHGADESFVERVCDLI
                     QATRIPQHPTDLLSEILCDADLDYLGRDDFEEISNHLY"
     assembly_gap    436..445
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <446..1183
                     /locus_tag="P5292_13020"
     CDS             <446..1183
                     /locus_tag="P5292_13020"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HRS40094.1"
                     /translation="EEFPAMRLFADFLKRRNPAAACPLSFLVLRSIRPRFPDLLLLVL
                     LGISLVGVGFAGQGPARDRTSLSHLSLPDHELLREGDVVLRRGKGIISESLRHFSLQD
                     PSYSHAGILVREQDRWMVVHAIGGEDNPTDKLKKESLAAFCRSDLSSAMGIIRLDLTP
                     ADRVRLKRIVESMLEAGPYFDSDFDLASDDRFYCTEFVYKAVLAATGDRKFLPLTRLS
                     GREYVACDNLYLNEHARIIYSYSSNRK"
     gene            1194..>1384
                     /locus_tag="P5292_13025"
     CDS             1194..>1384
                     /locus_tag="P5292_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HRS40095.1"
                     /translation="MKKLQHFLSFLLIAALSACGGGDSPKSVAENFLKAMNSMDFEGA
                     KKYGTEDTGKLLDMMSGFA"
BASE COUNT          305 a          364 c          382 g          323 t
ORIGIN      
        1 ggtagagatg attggaaatc tcctcgaagt catcccgtcc gaggtaatcc agatcggcat
       61 cgcacagtat ctccgaaagc aagtcggtcg gatgttgagg aatgcgcgta gcctgaatga
      121 ggtcacacac tcgttcgaca aaggattcat ccgcaccgtg ttggggcaat aagatgcgcg
      181 cgatcgcgca actatgccat tcgtggtcgg cgtaggcaac cgtgaaaccg gtgtcgtgca
      241 ggatcgctgc ggcccccagg agttccagct cgaatggcga gaggccttct tcacgaccta
      301 tgcgcaaggc ggaccgcaat acatcccggg tgtgggcagg tgagtggtaa tagaggtccg
      361 gcaaaagccg cgactgcagc atctgcgcta cctgtatatc cagcagccgg atattcacca
      421 tacgaggaaa agtacnnnnn nnnnngaaga atttcccgct atgcgattgt tcgctgattt
      481 tttgaaaagg cgaaacccgg cagcggcttg tccgttatca ttccttgtgc tgcgctccat
      541 ccgcccgcgt tttcctgacc tgttgctcct ggtactcctg ggtatatcgt tagttggggt
      601 cggtttcgcc ggacagggac ctgcccgcga ccgaacgtcg ctttcccatt tgtccctgcc
      661 ggatcatgaa ctgcttcgcg aaggcgatgt cgttcttcgc cgcggcaaag gtatcatcag
      721 tgaatccctc cgtcactttt cgttgcagga tccatcgtac tcccatgcgg ggatactcgt
      781 gcgggagcaa gatcgttgga tggtggtcca tgccatcggc ggggaagaca atccgacgga
      841 taaattgaag aaagaatcgc ttgccgcgtt ttgtcgttcc gatctgagta gcgccatggg
      901 tatcattcgt cttgacctca cacccgctga tcgggtgagg ctgaagcgga tcgtggaaag
      961 tatgctggag gcgggacctt atttcgattc cgatttcgac ctggcttccg atgaccggtt
     1021 ctactgcaca gagtttgttt acaaagccgt attggcagca actggggatc gtaaattcct
     1081 acctttgacc cgtctttcgg gtcgggaata tgtggcatgc gataacctgt acctcaacga
     1141 acacgcacgc atcatctatt cgtactcttc caaccggaaa taatccactt gaaatgaaaa
     1201 agctccaaca cttcctgagt ttcctgctga tcgcggccct gagtgcttgc ggtggaggag
     1261 attccccgaa atccgtagcc gagaacttcc tgaaggccat gaacagcatg gactttgaag
     1321 gcgccaagaa atacggcacg gaggacaccg gcaagttgct cgacatgatg agcggttttg
     1381 ccaa
//
LOCUS       DAOTON010000815         1383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_75410_length_1383_cov_3.150602, whole genome shotgun sequence.
ACCESSION   DAOTON010000815 DAOTON010000000
VERSION     DAOTON010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75410_length_1383_cov_3.150602"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="P5292_13030"
     CDS             complement(<1..285)
                     /locus_tag="P5292_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40096.1"
                     /translation="MLRFFEPWLQALDEVLRKCKRRPDPAEAFYKSDARSILFRLEAL
                     SRIGRGMLDKKAFDPLYDRFKQLEDILGAMDHAEAMLEQIGGIKGLAKFAG"
     gene            complement(328..726)
                     /locus_tag="P5292_13035"
     CDS             complement(328..726)
                     /locus_tag="P5292_13035"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRS40097.1"
                     /translation="MAHPPRPNLFLSIAGMKCPACREHSVFTHPNAYDLKHVGDMPAT
                     CPVCGQDLRVEPGFYFGAAYVSYALMVGLMIITAAIFYLVMGGIGDYIWTFLGIATAV
                     AILTTPLVFRYSRVIFLYICVKYKGVPSRR"
BASE COUNT          365 a          376 c          340 g          302 t
ORIGIN      
        1 gccggcgaac ttcgccagcc ctttgatccc gccgatctgt tcaagcatcg cttcggcatg
       61 gtccatcgcg ccgaggatgt cttcgagttg cttgaaacgg tcgtacaacg gatcgaacgc
      121 ctttttatcc agcatgcccc gaccgatacg actcagcgcc tccaggcgaa aaaggatcga
      181 gcgggcatcg ctcttgtaaa aagcttccgc cggatcagga cgacgcttgc atttgcgcag
      241 gacctcgtcc agcgcttgta accagggttc gaaaaatcgg agcatgacgt tcgtttcgca
      301 ttccaagata cgaaaggaac cccagcctca tcgacgcgaa ggcacgcctt tgtacttgac
      361 gcagatgtaa agaaagatca cgcgcgaata ccggaacacc agcggcgtgg tcaggatcgc
      421 gacggccgtg gcgatcccca ggaaggtcca gatgtaatcg ccgatgccac ccatcaccag
      481 gtagaagatc gcagcggtga tgatcatcaa gccgaccatc agcgcatagc tcacatacgc
      541 ggcaccgaaa taaaaaccgg gctccacgcg aaggtcctgc ccacataccg gacaagtggc
      601 aggcatatcg cccacatgct tcaggtcgta agcgttcgga tgcgtaaaaa cagaatgttc
      661 ccgacaagcc ggacacttca taccggcgat cgagagaaac aaattggggc ggggaggatg
      721 agccattgtg tggacaaaga taagtcagtc cgggaataac tgccggtgac tttggtcact
      781 ggcggaagtt tagcgaatag ccaatagcga atagcgaata gtggtggtgt gcatggggaa
      841 cctttctgca ttgatcacac ttttgcctta gagcgtgtgc catgcgcatg acaggatttt
      901 catgtttgaa tacttaacga taacaggcca aatcaagaac cccgagcctg ttttctgata
      961 tttgagtgta ggtgagcacg catctccggt gtcaattcaa caaccggaaa cccgaaactc
     1021 gaaactcgga accccaaaca agctctattc gctgttcgct attggctatt cgctaacaag
     1081 accaaaatcc cccaaatccc ccgattttcc tgacttttat cagttgctgc gcatacccct
     1141 tttagcttaa atttaaggtg cagttaggga atcaagggaa gggaggaaag tagggatgtc
     1201 aacctgatca cccatgaata cacggcccgg ctttttttcg ccagcttttt caggccactc
     1261 accatgaccc ggttctcatt ttatccgggc cctgctccca ctttgctctt ccacatccat
     1321 atgcaatatt aaaaagcact cgccatgaaa gtttatgatg acaaacacat caagaacgtc
     1381 gtg
//
LOCUS       DAOTON010000816         1375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_76075_length_1375_cov_3.075758, whole genome shotgun sequence.
ACCESSION   DAOTON010000816 DAOTON010000000
VERSION     DAOTON010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76075_length_1375_cov_3.075758"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1375)
                     /locus_tag="P5292_13040"
     CDS             complement(<1..>1375)
                     /locus_tag="P5292_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40098.1"
                     /translation="LDLVKYDEFRNYVWSVSYQLTDTLLYVTDGCSYGDQGYLFAASV
                     HSGNANTSELLLIRTDTAGNMLWNRRYSIGLRCDLSSLVPVADGGFIAGGTVLTDSTA
                     RYEDMLVIRGNEDGNLLWSQSIGSSARESGNSMIRTSDDQYAIAGTTEDANGSSGAFL
                     SKLDTSGNYYWFTRYATGIYDAAYSLVQADNGSYQLAGKGGPTGEDVLLLRTDVNGTP
                     SFARIYQLSFGTYQLNETALGMTGTPDGGFLIGGEVLRPGTLNPWLFLVKFSGNLLTE
                     WFNSYAFSTSRDQRFGSMLQQADGGVLLGGSRRISAAYPWNALTIATDASGISGCLET
                     ALNEHDSTVTPIAINLSPVSNTTVPTAYYSQLTAVQPNWTTDSACYTFTNIGQTNTSL
                     ETLSFYPQPATQQLSIREPGLLKFPCSYHIFDQVGRSSLQGTLDSIDSTLPLTSLTAG
                     IYQVWLVG"
BASE COUNT          309 a          350 c          398 g          318 t
ORIGIN      
        1 cctaccagcc atacctgata gatgcctgct gtgagtgagg tgagcggcag ggtggagtca
       61 attgaatcga gtgttccttg aaggctgctt cgccctacct gatcgaagat gtgatagctg
      121 caggggaatt tcaagaggcc gggttcccgt atgctgagtt gttgagtagc cggctgtgga
      181 taaaaggaaa gtgtctcaag cgacgtgttc gtctgaccga tgttggtgaa agtgtagcaa
      241 gccgaatcag ttgtccagtt cggttgcacc gccgtcaatt ggctgtagta cgcggtagga
      301 actgtcgtgt tgcttacagg tgaaaggttg atcgcgatcg gggtaacggt agaatcgtgt
      361 tcgttcaaag cagtctccag gcaaccactg atgccggatg catcggttgc gatcgtcagg
      421 gcattccagg ggtaggcagc ggaaatgcgc cggctgccac ccagtaatac tccgccatcg
      481 gcttgttgca acattgaccc gaagcgctga tcgcggctgg tgctgaatgc gtatgaattg
      541 aaccactcgg tgagcaggtt gccgctgaat tttaccagga acagccaggg attcaaggtc
      601 ccgggccgaa gtacttcacc accgatcaga aatcctccat ccggtgtgcc cgtcatgccc
      661 agcgccgttt cgttgagttg ataggtcccg aaactgagtt ggtaaattcg ggcgaaggaa
      721 ggagttccgt ttacatccgt gcggagcagc aatacatctt ctccggtagg tccgcctttt
      781 cctgccaact ggtagctgcc attgtcagcc tggacaagcg aataggcggc atcgtagatc
      841 ccggttgcgt agcgcgtgaa ccagtaataa ttcccggagg tgtccagctt gctcagaaac
      901 gccccgctgc tgccattggc gtcttccgta gttccggcaa tggcgtactg atcgtcgctc
      961 gttcggatca tcgaattgcc tgattcacgc gccgaggaac cgatagattg tgaccagagc
     1021 agattgccat cctcgttacc gcgaatgacc aacatatcct cgtagcgggc agtggagtcg
     1081 gtcaacaccg tgcctccggc tatgaagccc ccgtcggcca cgggtaccag gctcgacaaa
     1141 tcacagcgta gcccgattga atatcgccgg ttccaaagca tgttccctgc ggtatccgta
     1201 cgtatcaata acagttcgga tgtgttggcg ttaccggaat gtacgctcgc ggcaaacagg
     1261 tagccttgat ccccgtagga gcaaccgtca gtaacataca ataaggtatc ggtcagttga
     1321 taggaaactg accagacgta gttgcggaat tcatcgtatt tgacaagatc gaggt
//
LOCUS       DAOTON010000817         1373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_76266_length_1373_cov_2.789833, whole genome shotgun sequence.
ACCESSION   DAOTON010000817 DAOTON010000000
VERSION     DAOTON010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76266_length_1373_cov_2.789833"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..649
                     /locus_tag="P5292_13045"
     CDS             134..649
                     /locus_tag="P5292_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS40099.1"
                     /translation="MSLPSNRSVLIVEDNRYYASMIENHLKQYPGFTVTAFDNGDAAM
                     EAYDRVRPDIVLLDYHLNVEDDTAPTGYDYLQQIKDRNAEQAVVMLSSNDNVKQVVEL
                     LKTGAYDYVLKDESAFEQLDKVLINLEEVLRIRNEVKSLKTILRRQNRRFAWTLAAVA
                     VIVTACFEWLD"
     gene            complement(770..1114)
                     /locus_tag="P5292_13050"
     CDS             complement(770..1114)
                     /locus_tag="P5292_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRS40100.1"
                     /translation="MPYNEKLADRARAILAESGRKIKEKKMFGGLCFMVNDKMCIGVE
                     QDRMMVRLDPGRYEEALDQEGAAPMDFTGKVMKGFIFVDANTLTSKPKLNYWIKLALE
                     YNKVAKKAKKKK"
BASE COUNT          309 a          360 c          346 g          358 t
ORIGIN      
        1 ttttactggc ggaagggctg accatcgcat acttggtccg ttcccgtaaa aacctgcgcg
       61 cgcgcgccga gcgattcggc gacctccgcc gcaggaaccg cttcgtcgca cgcctgctgc
      121 atttctgata ttcatgtcgc tgccctctaa ccgctccgtc ctgatcgtcg aagacaaccg
      181 ctactacgcg tcgatgatcg agaaccattt gaaacaatat cccggcttca cggtgaccgc
      241 gttcgacaac ggggatgccg ccatggaagc ctacgatcgg gtgcgtcccg atatcgtact
      301 gctcgactat cacctgaacg tcgaagacga taccgcgccg accgggtacg actacctcca
      361 gcagatcaag gaccgcaacg cggaacaggc cgtggtcatg ctgagcagca acgacaacgt
      421 gaagcaggtc gtggaactgc tcaagacggg cgcctacgat tacgtgctca aggatgaatc
      481 cgccttcgag caactcgaca aggtgctgat caacctggag gaagtgctgc gcatccgcaa
      541 cgaagtgaaa agcctcaaaa ccatcctgcg ccggcagaac cgccgtttcg catggaccct
      601 ggccgccgtg gcggtgattg tcaccgcctg ctttgagtgg ctggactgag gagtttttat
      661 tgaaagaaag gcaagtgcca aggttcattt gccagtaact ggtaactagt aactggtaat
      721 tagtgattgg taattagtat ttaggaatta cctccttcgg aaaacgcatt tactttttct
      781 tcttcgcctt ctttgccact ttgttgtatt ccaaagccag cttgatccag taattcagtt
      841 tgggttttga cgtgagggtg ttcgcatcga caaagatgaa acctttcatg accttgccgg
      901 tgaaatccat cggagcggcg ccttcctggt caagggcttc ttcgtagcgc cccggatcca
      961 gccggaccat catgcggtcc tgttcgacgc caatgcacat cttgtcgttg accataaagc
     1021 acaatccgcc aaacatcttc ttttctttga tctttcgacc gctttccgcc agaattgccc
     1081 gggcgcggtc agcgagtttt tcgttgtaag gcatcgtggt gggataggtt gaaaaacatt
     1141 gttatgttac cgggaagtta ggatatttct gccaatacag attcaatgtc ttttcttcta
     1201 atggtagcca atcgaaagga attttttctt aacacaagat tcatgggttt ggactatcgg
     1261 gtcctgctcg tagtaatcgt ctgtactagt tttttactcc tttcaccatg gggaagccct
     1321 attcttaatt cagacggagc cattgaggtg ctgatggcat tttggtatcg ttt
//
LOCUS       DAOTON010000818         1373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_76295_length_1373_cov_2.100910, whole genome shotgun sequence.
ACCESSION   DAOTON010000818 DAOTON010000000
VERSION     DAOTON010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76295_length_1373_cov_2.100910"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1373
                     /locus_tag="P5292_13055"
     CDS             <1..>1373
                     /locus_tag="P5292_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40101.1"
                     /translation="QTSGKFNGQFYTLSAGLDSIIVGTNFTFNSATATDYFSGNRGNG
                     ITAITCGGNWNVLNSGNGTRQGVVCVDSSASVLNFTVTGNFVQNGGRFIGSERGSGNI
                     NITVNGILDMNAGTFRGILNTTNSVPANATFLAGSIDYDGGLFSVYHAANNNGSAGTM
                     SVLGNCKVNFSAVSDEFYFIGIPQVIPDVNTLILTLTISGTLTISGDNGKFVSSEAGN
                     TETVTIGNINISNGRNSFNGEQGSTYSNGHLVNLTVNGAVNISGGTTFFSAFTFPITA
                     TINGDITITGGDLSIKGDANSTVSNVNVLGGFFMSGGNFYFHNNASVPVSSSINMTVN
                     SNDDLVGDFVHTAGNIYFDNGNPSSNYLKLFIKSPSITWSGSGQITTSNPGTGTTNGY
                     IIFNRAGTSTFNRSGNHLIQQVYMTVESGNTLDLAIGDIQLASHPTMAGLPLFLMVNN
                     NGVLNMR"
BASE COUNT          356 a          360 c          322 g          335 t
ORIGIN      
        1 atcaaacctc cggaaaattc aacgggcaat tttatacgct aagtgccgga ctggattcca
       61 tcatcgtagg caccaacttt acattcaact ccgccactgc gaccgactat ttcagcggca
      121 accgcggcaa tgggatcaca gccatcacgt gtggaggaaa ctggaatgta ttgaattcag
      181 gtaacggaac acgacaggga gttgtctgcg tcgacagctc agcctctgta ttgaatttca
      241 ctgtgaccgg aaacttcgtc cagaatggcg gccgtttcat cggctcggag cgcggtagcg
      301 gcaatatcaa tattaccgta aacggtattc tggacatgaa tgccggtaca ttccggggca
      361 tcctgaatac taccaattcc gttcccgcta acgcgacctt cctggcagga agtattgatt
      421 atgacggcgg actgttcagt gtctatcatg ccgccaacaa caatggttcc gcaggcacca
      481 tgtccgttct gggaaattgc aaagtgaatt tttccgctgt cagcgacgaa ttttacttta
      541 tcggcattcc gcaagttata ccggatgtaa acaccctcat ccttacgctc accatttccg
      601 gcacactgac catcagcgga gacaacggga agttcgtttc gagcgaagcc gggaatactg
      661 aaacggtcac gattggcaac atcaacatct ctaacgggcg aaacagtttc aacggagagc
      721 agggctcaac ttacagtaat gggcacctgg tcaacctgac agtcaacggt gctgtcaaca
      781 tcagtggcgg gaccactttc ttttcagcat tcactttccc gatcactgct actatcaacg
      841 gcgacatcac catcaccggt ggagacctat cgatcaaggg tgacgccaac tcgaccgttt
      901 ccaacgtgaa tgtgctcgga ggcttcttca tgtccggcgg gaatttctac ttccacaaca
      961 acgcgtctgt tcccgtgagc agttcgatta atatgacggt taactcgaat gatgatctgg
     1021 tgggtgattt tgtccatacc gccgggaaca tctactttga caatggaaac ccgtccagca
     1081 actatctgaa gctctttata aaaagtccga gtatcacttg gtcaggaagt ggtcagatta
     1141 cgacgagtaa tcccggtaca ggaacaacca atggctatat catttttaac cgtgccggta
     1201 cgtccacctt taatcgatct ggcaaccact tgattcagca agtttatatg accgtggagt
     1261 ccggcaatac gctggacctt gcaataggag acattcaatt ggccagccat cctaccatgg
     1321 ccggattgcc tcttttcctg atggtgaaca acaacggggt actgaacatg cgt
//
LOCUS       DAOTON010000819         1372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_76337_length_1372_cov_4.118451, whole genome shotgun sequence.
ACCESSION   DAOTON010000819 DAOTON010000000
VERSION     DAOTON010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76337_length_1372_cov_4.118451"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..>1372)
                     /locus_tag="P5292_13060"
     CDS             complement(228..>1372)
                     /locus_tag="P5292_13060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS40102.1"
                     /translation="GLEKVVHEFHNWTNFLPVMADADRNGNVYVTLCFGDSINLHFSH
                     EPPNYLSAKPYDYTNPPSQESSNRDLLIIQYDVNGNYLRHRRFHSDRVSSEKNLDADV
                     LPNDHLYVLAAYDREFSVDDSTWNPPGIDSLYDYNLILLELDDQLNLVRHRRFHNNIL
                     SPRGHYNYLEADECGNLLVRIDRVMYNGFPFPPFDDNSLPILYKENTYVFNEALDVID
                     TISFPDFNLYDFLSADIHNGHLAMTGTFADDFHVEHFTVFERNPVPSNSYYDNFLLLG
                     DYTPCRLPIPEPLPEVIIPNVFTPDGDGLNDTWQVRDTTGRFAARWQIFNRWGQEVSH
                     GSFPGSGWTGTDERRQPCPDGVYFYILAIENSSIHQEERGWVKLIR"
BASE COUNT          354 a          321 c          361 g          336 t
ORIGIN      
        1 aacgaatgtt tatacaccaa ctttggcgaa aggtggttcg ggcatcttct gatatggaaa
       61 ggaagaaacg attgcaggaa ctgggtagta tgatccgaaa atcctacaac ccgaacgata
      121 atcgtattat ggaatacttc gacttcctga gctggatcga ctcccgggtt cgaaacgtcc
      181 catttgcgga agcacttcga gaaaaacgtc cggttcggcg atgaccgtca ccggatcagt
      241 ttcacccagc cacgttcttc ctgatggatc gaggagtttt cgatggccag tatgtagaaa
      301 taaactccgt cgggacaggg ttgtctgcgt tcatccgttc cggtccagcc ggaaccggga
      361 aatgaaccgt gtgatacttc ctggccccag cgattgaata tttgccaacg ggcagcgaaa
      421 cgccccgtcg tatcgcgcac ctgccaggtg tcgttcagtc cgtcgccatc tggcgtgaat
      481 acatttggaa tgataacttc cgggagcggt tctggtattg gaagcctgca aggcgtatag
      541 tcgccaagca acaggaaatt gtcgtagtag gaattcgatg gaaccgggtt tcgctcgaaa
      601 acggtaaagt gctcgacgtg gaaatcgtcg gcaaacgttc cggtcatggc cagatggccg
      661 ttgtgtatat cggcggacag aaaatcgtag agattaaagt cgggaaacga aatcgtatcg
      721 atcacatcaa gcgcttcatt gaatacataa gtattctcct tgtacaatat cggaagtgaa
      781 ttgtcatcaa agggcggaaa cgggaagcca ttgtacatga cccggtcgat ccgcacgagc
      841 agattgccgc attcgtcggc ttccaggtaa ttgtaatgac ccctcggcga cagtatgttg
      901 ttatgaaagc gccggtgccg tactaggttt aactgatcgt ccagctcgag taggatgagg
      961 ttgtaatcat acaacgaatc gattccggga ggattccagg tgctgtcgtc cacgctgaat
     1021 tcccggtcat aggccgccaa tacatagaga tgatcgttcg gcagcacgtc cgcgtcgagg
     1081 ttcttttcgc tgctaaccct gtcagaatga aaccgccgat gacgcaaata attaccgttc
     1141 acgtcatatt gtatgatcag cagatcacgg ttagagcttt cctgactcgg cggatttgtg
     1201 taatcatagg gcttagcact taggtagttt ggtggttcat gcgaaaagtg aagattgatg
     1261 ctgtctccga aacacaatgt gacatagacg ttaccgttcc ggtcagcatc cgccatgacg
     1321 ggcagaaaat tcgtccaatt gtggaattca tgaacaacct tttccagacc at
//
LOCUS       DAOTON010000820         1369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_76642_length_1369_cov_3.282344, whole genome shotgun sequence.
ACCESSION   DAOTON010000820 DAOTON010000000
VERSION     DAOTON010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76642_length_1369_cov_3.282344"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1369
                     /locus_tag="P5292_13065"
     CDS             <1..>1369
                     /locus_tag="P5292_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40103.1"
                     /translation="VGCGDVTIRDFTVQYTIPSTQSGQIDLATYTLTVNGDLNFAGTC
                     NCTGASGGGLLTEGLIDIASTTQQQIVDIRAANNSYPGTICKLRINKVQPVAAGNAQE
                     ALLKGNLNILYNFDIANGVLLSQNGGTYDADENTGVNFKTINILNDDVNAVTRQSIPT
                     QDTRNGFFEGRLNRKITNVGTKNEYLFPLGFRRKTFSGALGDYFYTPSLISFNSVTNA
                     NRYVVGTFLNSNNNFMVDGVWIGTTGHGCGNPFEIDDQGGPTASTCLNKEIDMIGDYY
                     WDFQENNGPNVTGDPAISTGALGAVDYDIQLVGDKVALMALDGLSGSELRVVKRPSVA
                     VPDSTGQGAWITTQGTHSGTDLSVNTGMSMYSVSNLFLQGGRRDGLTSFSGFALTGNG
                     PSPLPVELLEFSARKQGSASVLCEWSTASETNNAYFDVEVSRGNEDGLSFKAIGRVPG
                     RGTTTE"
BASE COUNT          317 a          401 c          375 g          276 t
ORIGIN      
        1 cgttggctgt ggagatgtca ccatccgtga ttttacggtc cagtacacca tcccttcgac
       61 acaaagcggg cagatcgacc tcgccaccta taccctgacc gtcaacggcg acctcaattt
      121 cgccggcacc tgcaattgca ccggggccag cggaggcggc ttgttgaccg aaggactgat
      181 cgacatcgcc agcaccacgc aacagcagat cgtcgatatc cgcgcagcga acaacagtta
      241 cccgggaacc atctgcaagt tgcgcatcaa taaagtgcag cccgttgcag ccggcaatgc
      301 acaggaagcc ctgttaaaag gtaacctgaa catcctctac aacttcgata tcgcgaacgg
      361 ggtcctgttg tcgcagaacg gcggtactta tgacgccgat gagaataccg gtgtcaattt
      421 caagacgatc aacatcctca acgatgacgt gaacgccgta acccggcaat ccatcccgac
      481 gcaagacaca cgcaacggtt tcttcgaggg ccgcctgaac cggaagatca caaacgtcgg
      541 caccaagaac gaatacctgt tcccactcgg cttccgccga aaaaccttca gcggcgcatt
      601 gggcgattat ttctatacac cttcgctgat ctcgttcaat tccgtgacca acgcgaatcg
      661 ttatgtggtc ggtacgttcc tgaacagcaa caacaacttc atggtcgacg gtgtctggat
      721 cggcaccacc ggccacggtt gcggcaatcc gttcgagatc gacgaccagg gcgggccgac
      781 cgcttcgacc tgcctgaaca aggagatcga tatgatcggc gactactatt gggacttcca
      841 ggagaacaac ggtccgaacg tgactggtga tcctgccatc agcacgggag cactcggtgc
      901 ggttgattac gatattcaac tggtaggcga taaagtggcg ctgatggcgc tcgacggctt
      961 gagcggatcg gaactgcgcg tggtcaagcg tccgagtgta gccgtgcccg acagcaccgg
     1021 ccagggggct tggattacga cccagggaac acattccggt acggatctca gcgttaacac
     1081 gggtatgtcc atgtactcgg tatccaacct cttcctgcag ggtggtcgtc gcgatggact
     1141 cacgtccttc tccggcttcg cgctgacggg taacggacct tctccgctgc cggttgaact
     1201 gctcgagttc agcgcgcgga agcagggtag tgcctcggtg ctttgcgaat ggtcaaccgc
     1261 ttcggagacc aataatgcct acttcgacgt agaagtatcc cgtggcaatg aagacggttt
     1321 gtcattcaag gcaatcggtc gtgttcctgg tcgcggaacc accacagag
//
LOCUS       DAOTON010000821         1363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_77186_length_1363_cov_2.290520, whole genome shotgun sequence.
ACCESSION   DAOTON010000821 DAOTON010000000
VERSION     DAOTON010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77186_length_1363_cov_2.290520"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="P5292_13070"
     CDS             <1..554
                     /locus_tag="P5292_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRS40104.1"
                     /translation="RPELPATLTIQGAFPTFRYRQTDALLKGLDAQVRYRPLRRLQLS
                     GKASLLRARNLNAAEWLVMMPADRAELEVSFLPSARPRFRDNSVSIAAQYVARQTRVP
                     DNSDFVAPPADYLLLRFEASTTLNLNRQKIVVGLGLSNLLDTVYRDYLDRFRYYTDAM
                     GRNIVLRLRIPLVFTKAETPTTE"
     gene            564..1124
                     /locus_tag="P5292_13075"
     CDS             564..1124
                     /locus_tag="P5292_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40105.1"
                     /translation="MKLHQLLTIGAFSLLALQSCKKDDNEVSSPAPPANESEVITTLR
                     ITFTDSANTADVRTAEFRDPDGPGGVAYDRFDTIRLDSNCTYFATILLLNETASPVDT
                     ISNEVLDEADEHLFCFTPSGTSASVTVTDRDGNNLPIGLQSTWITTSAGSGTMNVSLH
                     HQPGIKNGDCALGETDVEVLFVVEIE"
BASE COUNT          310 a          457 c          344 g          252 t
ORIGIN      
        1 tgcggcccga gttaccagcg acgctgacga tccagggcgc gttccccacc ttccgctacc
       61 ggcagaccga cgccctgctg aaaggcctgg acgcgcaggt ccggtatcgt ccgctgcggc
      121 gcttgcagct cagcggaaaa gcgtccctgc tgcgggcgcg caacctgaac gcggcggaat
      181 ggctggtgat gatgccggcc gaccgcgcgg aactggaagt gagtttcctt ccgtcggctc
      241 gtccgcggtt ccgcgacaat agtgtgagca tcgccgcgca gtatgtagcg cgacaaacac
      301 gggtacccga taacagcgac ttcgttgcgc cgccggcgga ttacctgctg ctgcgtttcg
      361 aagcttccac caccctgaac ctgaatcgcc agaagatcgt cgtaggactc ggcctgagta
      421 acctgctcga caccgtctac cgcgattacc tcgaccggtt ccggtattac accgatgcca
      481 tgggacgcaa catcgtcctg cggctgcgca tcccgcttgt gttcacaaaa gcggaaacac
      541 caaccaccga ataatccaac tccatgaaac tccatcaact cctgaccata ggcgccttct
      601 ccttactcgc attacaatcc tgcaagaagg acgacaacga agtttcctca cccgcccctc
      661 ctgcaaacga atcggaagtc atcacgacgc tgcgcatcac cttcaccgat tcggcgaaca
      721 cggccgatgt acgcaccgcc gagttccgtg atcccgacgg tccgggcggt gtggcctacg
      781 accgcttcga caccatccgg ctcgacagca actgtaccta cttcgcaacg attctcctgc
      841 tcaacgaaac ggcctcaccg gtcgacacga tctccaacga agtactcgat gaagccgatg
      901 agcacctctt ttgcttcaca ccatcgggca ccagcgcgtc ggttaccgtc accgaccgtg
      961 atggcaacaa cctcccgatc ggactgcaat cgacctggat caccaccagc gccggttccg
     1021 gcaccatgaa cgtttcccta caccaccaac ccggcatcaa gaacggcgac tgcgcattgg
     1081 gcgaaacgga tgtggaggtt ttgtttgtgg tggagattga gtgagaggga cgaggggcgt
     1141 ggggcgaggg gcgagggacg agggacgagg taaggtcgta ctaagcaacc caacaacatt
     1201 caacattcaa ctttcaactt tcaactttca actttcaact ttcagccttc aaccttcaac
     1261 tcaacacgaa acccgaaacc cggaacccga aaccccaaac tcacttcctc ccccaggaat
     1321 ccttcaacga caccgtccgg ttaaacacca gcttgccgtt cgc
//
LOCUS       DAOTON010000822         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_77426_length_1360_cov_3.113410, whole genome shotgun sequence.
ACCESSION   DAOTON010000822 DAOTON010000000
VERSION     DAOTON010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77426_length_1360_cov_3.113410"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="P5292_13080"
     CDS             <1..858
                     /locus_tag="P5292_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRS40106.1"
                     /translation="KTELDNFLHKHPETAKALEQKILQSERERKEIAGIKKLANERAR
                     KANLHNRKLRDCRVHLNSRHIDRQESTLFITEGDSASGSITKSRDVNTQAVFSLKGKP
                     LNCYGLTKKVVYENEEFNLLVNALNIEEGMEELRYNKIVISTDADVDGMHIRLLLLTF
                     FLQFYPELVKNGHVYILQTPLFRVRNKKETRYCYSEQERVRAIEALSPKPEITRFKGL
                     GEISPDEFAHFIGKNIRLEPVMLGQEETIHQLLDYYMGKNTPDRQEFIITNLRIEKDE
                     VKKTEEIEA"
     gene            879..>1360
                     /locus_tag="P5292_13085"
     CDS             879..>1360
                     /locus_tag="P5292_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007368150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRS40107.1"
                     /translation="MAKKKLQEETNPAAAPAGHETRMIHVNGMYQNWFLDYASYVILE
                     RAVPHINDGLKPVQRRLMHSMWEMEDGRYNKVANIIGHCMKYHPHGDASIGDALIQIG
                     QKDLLIDTQGNWGNIYTGDSAAAPRYIEARLSKFALEVVFNEKTTNWQLSYDGRNKEP
                     "
BASE COUNT          385 a          381 c          353 g          241 t
ORIGIN      
        1 aagaccgagc tagacaattt cctgcataag caccctgaaa ccgcgaaggc cctcgagcag
       61 aagattctgc aaagcgaacg cgagcgaaaa gaaattgccg ggatcaagaa actggccaac
      121 gagcgggccc gcaaggccaa cctgcacaac cggaaacttc gcgactgcag ggttcacctg
      181 aattcccgtc acatcgaccg acaggaatcc accctcttca tcaccgaagg tgactccgct
      241 tccggttcca tcaccaagtc acgggatgtg aacacccagg cggtattcag cctgaaaggt
      301 aaacccctga actgctacgg cctgaccaag aaggttgtct atgagaacga agagttcaac
      361 ctgctcgtga acgcgctcaa catcgaagaa gggatggagg agttgcgtta caacaagatc
      421 gtgatctcga ccgatgccga tgtcgacggg atgcacatcc gattgctcct gctcacgttc
      481 ttccttcagt tttatccgga gttggtcaag aacggccacg tctacatcct gcagacgccg
      541 ctcttccggg tgcggaataa gaaagagacc cgctactgct acagtgaaca agaacgtgta
      601 cgggcgatcg aagccctgag tccaaagccg gaaatcaccc gcttcaaggg tttgggtgag
      661 atctcacccg acgaattcgc gcatttcatc ggaaagaata tccgactcga accggtgatg
      721 ctgggacagg aagagaccat tcaccaactg ttggattatt acatgggaaa gaataccccg
      781 gaccgacagg aattcatcat taccaatctg cgtatagaga aggacgaggt caagaagacc
      841 gaagaaatcg aggcctgatc cgcagtatgc tttagacaat ggcgaagaag aaactacagg
      901 aagaaaccaa ccccgctgcc gcacccgccg gtcacgagac ccggatgatc cacgtcaacg
      961 gaatgtacca gaactggttc ctggattatg catcctatgt aatcctggaa cgcgccgtcc
     1021 cgcacatcaa cgacggtttg aagccggtac aacgacgtct catgcattcg atgtgggaga
     1081 tggaagacgg ccggtacaac aaagtggcca acatcatcgg ccactgcatg aaataccatc
     1141 cccacggcga tgcttccatc ggcgacgccc tgatacagat cggacagaaa gacctcctca
     1201 tcgataccca gggtaactgg gggaatatct acacaggcga tagcgcggca gcgccgcgtt
     1261 acatcgaggc acggctctcg aagttcgccc tcgaggtggt cttcaacgaa aagacaacga
     1321 actggcagct cagctacgat ggccgaaaca aggagccgat
//
LOCUS       DAOTON010000823         1359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_77527_length_1359_cov_2.646472, whole genome shotgun sequence.
ACCESSION   DAOTON010000823 DAOTON010000000
VERSION     DAOTON010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77527_length_1359_cov_2.646472"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="P5292_13090"
     CDS             complement(<1..561)
                     /locus_tag="P5292_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40108.1"
                     /translation="MKMLLRVLAALTSLMLFARLSFAQTYTLNAASNGTTVNTCGGTF
                     YDSGGSGGNYGNNESYTMTFCSNSANCVQLNFTSFRTQGGNDILYLYDGPNTSAPLIG
                     NFSGTTSPGTVVSSTGCITVRFVSNGSNTRGGWAATVSCVACGTATNMSNTTVTTCSR
                     QFYDSGGSAGAYGNNQTFVTTFTSANS"
     gene            complement(802..>1359)
                     /locus_tag="P5292_13095"
     CDS             complement(802..>1359)
                     /locus_tag="P5292_13095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40109.1"
                     /translation="CSVLPIELLNFTAEAKNDRVDLQWSTSSETNNSYFTVERSQDAR
                     QFDPIQVVPGAGNSSTERYYLSSDFEPLQGISYYRLRQTDIDGKFTFSEPVAVRYSNT
                     VKSLTLVPNPANDRVQVIFESGMEVGGTLRVFSLEGKLMEQRQLQMRRGTNSLDLELT
                     DWKSGSYLVELVTADGVLRQRLIRN"
BASE COUNT          325 a          336 c          363 g          335 t
ORIGIN      
        1 actgttggcg gaggtgaacg tggtcacgaa ggtctggttg ttgccatagg cgccggcact
       61 accgccacta tcatagaatt gcctggaaca agtcgtaacg gtggtgttac tcatgttggt
      121 cgcggtacca caagcgacac aggaaactgt ggccgcccat cctccacgcg tgttggaacc
      181 gtttgaaacg aagcggaccg tgatacagcc ggtggaagaa accacggtac cgggacttgt
      241 ggttccggag aagttaccga tcaggggcgc tgaggtattg ggtccgtcgt agaggtagag
      301 aatatcgtta ccgccctgcg tccgaaagga ggtgaagttc aactggacac agttcgccga
      361 gttggaacag aacgtcatgg tataactctc gttatttcca tagttccctc cggagccgcc
      421 ggaatcatag aaagtacccc cacaggtatt gacggtagtt ccgttggatg ccgcgttcag
      481 ggtatacgtt tgggcaaacg ataaccgggc gaacagcatc aggctggtca gcgctgcaag
      541 gacacgaagg agcattttca tacacgaagc gttttaatgc aatggttccg ggatagtacc
      601 tgggcattat tccgggcttc gtgttttagc gaaatcgttg cttaatggtt gaatcaaaaa
      661 ccatgaatcc cctacatcga attgatcatt tttaaatcat catttttata aaaagccagt
      721 ataatgcgct aaatcattgc atgaatgagt ataaaaaaag cccggatcat tacatccggg
      781 cttcaacttt ctttcaactt atcagttcct gatcaatcgt tgacggagaa ctccgtcggc
      841 ggtgaccagt tccaccagat agctcccgct cttccagtcg gtcaattcca ggtcaaggga
      901 attcgtgccg cggcgcatct ggagttgtcg ctgttccatc agctttcctt caagagaaaa
      961 gacccgaagt gttcctccca cttccattcc actctcgaag atcacctgaa cgcgatcatt
     1021 ggccgggtta gggaccaggg tgagtgattt taccgtattg gaatagcgca ccgcgaccgg
     1081 ttcggagaag gtgaacttcc cgtcgatatc cgtttggcgc aaacggtagt aactgattcc
     1141 ctgcagtggt tcaaaatcgg aactcaggta ataccgttcg gtggaactgt taccggcccc
     1201 tggcacaacc tggatcggat cgaactgtcg cgcgtcctgg ctgcgttcga ctgtgaagta
     1261 gctgttattg gtttccgagc tggtcgacca ttgtaggtcg acccggtcgt tcttcgcctc
     1321 cgctgtaaag ttcagcagct cgatagggag tacactgca
//
LOCUS       DAOTON010000824         1357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_77689_length_1357_cov_3.370968, whole genome shotgun sequence.
ACCESSION   DAOTON010000824 DAOTON010000000
VERSION     DAOTON010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77689_length_1357_cov_3.370968"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..615)
                     /gene="frr"
                     /locus_tag="P5292_13100"
     CDS             complement(58..615)
                     /gene="frr"
                     /locus_tag="P5292_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRS40110.1"
                     /translation="MLDANKTLNDTRSHMQKAIDHLEVELAKIRAGRANPTMLDSVHV
                     DYYGTATPLNQVANVSAPEARTLVIQPWEKSMLGPIEKAILGANLGFTPSNDGTVVRI
                     NIPPLTEERRKELVKKTKAEGEHAKVGIRTVRRESNEHIKKEAKGVPEDVVKGLEDQI
                     QKITDGFIAQVDKHVEAKEKEIMTI"
     gene            complement(679..>1357)
                     /gene="pyrH"
                     /locus_tag="P5292_13105"
     CDS             complement(679..>1357)
                     /gene="pyrH"
                     /locus_tag="P5292_13105"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRS40111.1"
                     /translation="GESLMGEKEFGIDHTRLRQYAEEIKRIVDKGVETAIVIGGGNIF
                     RGVGNDAGGVIDRAQGDYMGMLATVINSMALQSALEGLGVKTRLQSAIKMEQICEPYI
                     RRRAIRHLEKGRVVIFGAGTGNPYFTTDTAASLRAVEINADVILKGTRVDGIYTADPE
                     KDKNAVKYETVTFDEVYEKGLKIMDLTAFTLCNENKVPIIVFDMNKPGNLLRVMEGQQ
                     VGTLVEF"
BASE COUNT          222 a          393 c          389 g          353 t
ORIGIN      
        1 tccctgtcac aagacccgaa actcgaaacc cggaacccgg aacccggaac ccgaaactca
       61 aatcgtcatg atttccttct ccttcgcctc gacatgcttg tcgacctggg cgatgaagcc
      121 gtcggtgatc ttttggatct gatcttcgag tcctttcacc acatcttccg gtacgccttt
      181 cgcttctttc ttgatgtgtt cgttggattc gcggcgaacc gtgcggatgc ccactttggc
      241 gtgttcgcct tccgcttttg ttttcttcac cagttccttc cggcgttctt ccgtcagcgg
      301 cgggatgttg atccgaacca ccgttccgtc attcgaagga gtgaagccga ggttggcgcc
      361 caggatcgcc ttttcgatcg ggcccagcat gctcttttcc cagggctgaa tcaccagtgt
      421 acgggcttcc ggtgcgctca cgttcgcgac ctggttcagg ggagtcgcgg taccgtagta
      481 gtcaacgtgc acgctgtcga gcatggtcgg gttggcgcgg ccggcgcgga tcttggcgag
      541 ttcgacttcc aggtggtcga tcgccttttg catatgcgaa cgggtgtcgt tcaaggtctt
      601 gttggcgtct aacataaaag cgacggtgtt gagcggttcg ttttcggtca gcgaggtaat
      661 ccgctgttta gttcaatatc agaattcgac cagggtgccg acttgctgtc cctccatcac
      721 acggagaagg ttgccgggtt tgttcatgtc gaacacgatg atcggtactt tgttttcgtt
      781 gcacagggtg aaggcggtca ggtccatgat cttgaggccc ttttcgtaaa cctcgtcgaa
      841 ggtgacggtc tcgtacttca cggcattttt gtctttctcc ggatcggctg tatagatgcc
      901 atccacgcgc gtacctttca ggatcacatc ggcgttgatt tcgaccgccc gcagggaggc
      961 ggccgtgtcg gtcgtaaagt aagggttgcc ggtgccggcg ccgaagatga cgacacgtcc
     1021 tttttccagg tggcggatgg cgcgacgtcg gatatagggc tcgcagatct gctccatctt
     1081 gatcgccgac tgcaggcggg tcttcacgcc aagtccttcc agcgcgcttt gcagcgccat
     1141 ggagttgatg accgtcgcca gcatgcccat gtagtcgccc tgggcgcggt cgatcacccc
     1201 gccggcatcg ttcccgacgc cgcggaagat gttcccgcca ccgatcacga tggcggtctc
     1261 tacgccttta tcgacaatcc gtttgatctc ttccgcgtat tggcggaggc gggtgtggtc
     1321 gatcccgaat tctttctcgc ccatcagcga ttcgccg
//
LOCUS       DAOTON010000825         1354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_77973_length_1354_cov_2.388761, whole genome shotgun sequence.
ACCESSION   DAOTON010000825 DAOTON010000000
VERSION     DAOTON010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77973_length_1354_cov_2.388761"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="P5292_13110"
     CDS             complement(<1..575)
                     /locus_tag="P5292_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HRS40112.1"
                     /translation="MKRFTFTDKKDTRSGFGAGLLELGRSNPQVVALCADLTGSLKMD
                     AFEKEFPDRFFQVGIAEANMMGLAAGMTVGGKIPFTGTFANFSTGRVYDQIRQSIAYS
                     GKNVKICASHAGITLGEDGATHQILEDIGMMRMLPDMVVINPCDFNQTKAATLAIAAY
                     KGPVYLRFGRPVVPNFTPADQVFKIGEAVTLA"
     gene            complement(591..>1354)
                     /locus_tag="P5292_13115"
     CDS             complement(591..>1354)
                     /locus_tag="P5292_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40113.1"
                     /translation="ENQLTTVEAIINSMTMQVNLLDVNGVPSETNVNMTFYDRNTGEV
                     KYNYIHTLNAKGNPDTLVIDPLPIYRIVVHTVPQAVKDSVVLVPGKHSTVGIDAGQGS
                     LMIRYDGPSEYRKIQAIVRQPGDMATLVVQDINATEKYRVGKYNLEILTLPRILLDNV
                     DISQSKTTTVTIPRPGIVTLVTNNPGFGSIYTVEGKTLKWVTNLDENLSKETFILQPG
                     RYRAVFRPRASRESIYTLEKEFSIVSGESNVVIMN"
BASE COUNT          265 a          338 c          426 g          325 t
ORIGIN      
        1 gccagggtta cggcttcgcc gatcttgaat acctgatcgg ccggggtgaa gttgggaacg
       61 accggtcgtc cgaagcgcag gtacaccgga cccttatagg cggcaatagc gagtgttgcg
      121 gctttggttt ggttgaaatc gcaggggttg atgaccacca tgtcggggag catacgcatc
      181 atgccgatgt cttccaggat ctggtgggtg gcgccgtctt cgccgagcgt aatgccggcg
      241 tgggaggcgc agatcttgac gttcttaccg gaatacgcga tcgactgacg gatctgatcg
      301 taaacacgtc cggtcgagaa attggcgaag gtacccgtga acgggatctt gccgccgact
      361 gtcatgcctg cagcgaggcc catcatgttc gcctcggcaa tgccgacctg aaaaaagcgg
      421 tccgggaatt ctttctcaaa ggcgtccatc ttgagactgc cggtcaggtc ggcgcagagt
      481 gcgacgacct gcgggttgct gcggcccagt tcgaggagcc cggcgccaaa gccggagcgg
      541 gtgtctttct tgtcggtaaa agtgaaacgt ttcatgcggg tggggtgggt tcaattcatg
      601 atgacgacat tcgattcgcc ggacacgatc gagaattcct tttcgagtgt gtagatactt
      661 tcccgggaag cccgcggacg gaagaccgcg cggtaacgac cgggttggag aatgaaggtt
      721 tctttgctga ggttttcgtc gaggttggtg acccatttca gggtcttgcc ttcgaccgta
      781 tagatactgc cgaagccggg attgttggta acgagtgtca cgatgccggg acgcggaatc
      841 gtgaccgtag tggttttact ctggcttata tctacgttgt cgagcaggat acgaggtaat
      901 gtaagaatct cgagattgta cttgccaacc cggtactttt ccgtcgcgtt gatgtcctgg
      961 acgacgaggg tagccatatc gcccggttgg cgaacgatcg cctggatctt ccggtattcc
     1021 gatgggccgt cgtaccggat catcagggag ccctgtccgg catcaatacc cacggtgctg
     1081 tgtttcccgg gtacgagcac caccgagtcc ttgacggctt gcgggacggt gtggaccacg
     1141 atccgataga tcggaagcgg gtcgatgaca agcgtgtccg gattgccctt ggcgttcagc
     1201 gtgtgaatgt aattgtactt gacctcgccg gtgttacggt cgtaaaacgt catgttcaca
     1261 tttgtctcgc tgggtacacc gttcacgtcg agcaggttca cctgcatggt catggaattg
     1321 ataatagcct cgaccgtggt cagttgattt tcca
//
LOCUS       DAOTON010000826         1352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_78128_length_1352_cov_2.474171, whole genome shotgun sequence.
ACCESSION   DAOTON010000826 DAOTON010000000
VERSION     DAOTON010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78128_length_1352_cov_2.474171"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="P5292_13120"
     CDS             complement(<1..180)
                     /locus_tag="P5292_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40114.1"
                     /translation="MSKLLTSLCVRSAGLFVLILPLLTTAQPAGTLDPSFGNAGRYVQ
                     DFGFQDNLTHVAVQPL"
     gene            complement(270..854)
                     /locus_tag="P5292_13125"
     CDS             complement(270..854)
                     /locus_tag="P5292_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HRS40115.1"
                     /translation="MSVEMQVRARANDRCELCSASNELSVYAVPPDEGPSADRAVLVC
                     QDCLHQLSGQDELTPSRWTFLKDSMWSEVPAVQVVAWRMLHKLKSESWAQDALDQLYL
                     DDELLQWAQATGDHEDRDPVAVHQDVNGARLQNGDTIVLTKSLDVKGSQINARIGTTV
                     KNIKLVPDNTEQIEGKIEGQQIVILTKYVRKTTA"
     gene            complement(933..>1352)
                     /locus_tag="P5292_13130"
     CDS             complement(933..>1352)
                     /locus_tag="P5292_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016129842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRS40116.1"
                     /translation="MAKNKTEPTQASVERFIRTFANSEQKKKDSYELIDLMKKVSGHE
                     PKMWGPSIIGFGSYHYKYASGHEGDAPIIGFSPRKAAISLYVYTGLDEHEHLLEGLGK
                     YKIGKVCIYVNKLSDIDRQKLIRLMKESIRYIKTKYP"
BASE COUNT          276 a          367 c          375 g          334 t
ORIGIN      
        1 cagcggctgc accgcgacgt gggtcaggtt gtcctggaaa ccgaagtcct gtacataacg
       61 accggcgttt ccgaaggaag gatccaacgt accggccggt tgcgcagtag ttaagagagg
      121 caggatcagc acaaagaggc cggctgatcg aacacaaagg gaggtaagaa gtttgctcat
      181 ccggcgaaga taaacccaac gcccggatca cgattagacg gggccgtaca actttcagga
      241 tttatcctgc gaatcggcca aattttcgtt caggccgttg tcttacgcac atatttggtc
      301 aggataacga tctgctgtcc ttcgatctta ccctcgatct gctccgtgtt atccggaaca
      361 agtttgatat tcttcaccgt cgtcccgatg cgggcgttga tctgggagcc ttttacatcg
      421 agcgatttgg tcagcacgat cgtatcgccg ttttgcaggc gcgcaccgtt cacatcctgg
      481 tgtacggcaa cggggtcgcg gtcttcatga tcgccggtcg cctgtgccca ttgcaacagt
      541 tcatcgtcga ggtagagctg gtccagcgca tcctgcgccc agctctcgct cttcagcttg
      601 tgcaacatgc gccaggctac cacctggaca gcgggaacct cgctccacat gctgtctttg
      661 aggaaggtcc agcgggaggg cgtcagttca tcttgcccgg aaagctggtg cagacagtcc
      721 tggcacacca ggacggcccg gtcggccgaa ggtccttcgt cgggcggtac cgcataaacc
      781 gataattcat tggaagcgct gcacaattcg cagcggtcgt tggccctggc tcggacctgc
      841 atttcaacgg acatgttaac gggtttgggg ctgcaaactt aagagaatgc cccgttccgg
      901 gaaaagacca tggtcattgg agccgatgtt ccttacgggt acttcgtctt gatgtaccgg
      961 atggactcct tcatgagccg gatcagtttc tgccggtcga tatcgctgag cttgttcaca
     1021 tagatgcaga ctttgccgat tttatacttt cccagtcctt ccaacaggtg ttcgtgttcg
     1081 tcgagtccgg tgtacacgta gagtgaaatg gccgcttttc gcggagaaaa gccgatgatc
     1141 ggggcgtcgc cttcatgccc gctcgcatat ttgtaatgat acgagccaaa cccgatgata
     1201 ctggggcccc acatcttcgg ttcgtgacca gagaccttct tcatcaggtc gatcagctca
     1261 tagctgtctt tcttcttctg ttcggaattg gcaaacgtgc ggatgaatcg ttcgacgctt
     1321 gcctgagtgg gttcggtctt gttcttagcc at
//
LOCUS       DAOTON010000827         1348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_78469_length_1348_cov_3.235112, whole genome shotgun sequence.
ACCESSION   DAOTON010000827 DAOTON010000000
VERSION     DAOTON010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78469_length_1348_cov_3.235112"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1348)
                     /locus_tag="P5292_13135"
     CDS             complement(<1..>1348)
                     /locus_tag="P5292_13135"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS40117.1"
                     /translation="NASGGNSYVWYPGGFTGPTISVDPSVSTSYTVVGTDAFGCTNSD
                     TVDVAVNSLPVATISPDVDICQGGSATLTAGGGSTYTWTPTGSTTGTITVNPGSSTTY
                     NVQVSDGNGCSTTASVNVNVHSLPPVNLQSSFLCAGSVTTLDAGPGSVSYYWTPTGDT
                     TRTLSVSTGGTYAVTLTDAWGCTTTASATINVGSSITINLGNVSFCQGDSATLDAGYS
                     GMNYQWSTGATTQAISVNTPGSYSVIVTDNSGCSGSIAVTANVNPLPVVSFSATSACL
                     GSATIFTNTSSIGSGSISSYSWNFGNGDTTLASNPSETYAAAGTYSVSLTATSVNGCI
                     SSITQPVQVNPLPVAAFTAPGACAGSPTTFTDNSSVSSGNIVTYAWNFGDGNNSNAHN
                     PVHSYSSPGNYTVDLTVTTAGGCTSSSTQTVAIHEIPVAGFVANPVCIGSPMTFANS"
BASE COUNT          287 a          349 c          411 g          301 t
ORIGIN      
        1 ggagttggca aaggtcatgg gacttccgat acaaacggga ttcgctacga agcctgcgac
       61 cggtatctcg tgaatcgcga cggtctgcgt gctgctgctg gtacagccgc ctgctgtcgt
      121 cacggtgaga tctaccgtat agtttccagg gctggaatag ctgtgaaccg gattgtgcgc
      181 gttggaattg tttccatcgc cgaaattcca ggcataggtg acgatgttgc cgctgctgac
      241 actggaattg tcggtaaacg tagtcgggga accggcacag gcgccgggcg ccgtgaaagc
      301 ggcgaccggt agcggattca cctgaacggg ctgcgtgatg ctgctgatac atccattgac
      361 actggttgcg gtaaggctta ccgaataggt gccggccgct gcatacgttt cgctgggatt
      421 gctggcaagc gtggtgtcac cgttaccgaa attccaactg tagctcgaga tcgatcctga
      481 tccgatgctg gaggtattgg taaaaatggt tgcactgcca agacaggcgg acgttgcgga
      541 gaaggatact accggaagtg ggtttacgtt cgcggtgacg gcgatcgatc cggaacaacc
      601 ggaattgtcc gttacaatta cactatagct acccggagta ttgaccgaga tggcctgtgt
      661 cgtagcgcct gtggaccatt ggtagttcat accagaatag cctgcgtcca gtgtcgcgct
      721 gtcgccctga cagaaactca cattacccag gttgatcgtg atggagctgc caacgttgat
      781 cgtcgctgag gcggtagtcg tgcagcccca ggcatcggtc aacgtgacgg cgtaggtgcc
      841 acctgtactg accgatagcg tgcgtgtcgt atctccggta ggtgtccagt aataggatac
      901 gctacccgga cccgcatcca gcgtggtgac cgagcctgcg caaaggaagg aggattgcag
      961 gtttaccggc gggagtgaat gaacattcac atttacgctg gctgtcgtcg aacagccatt
     1021 gccgtcggag acttgaacat tatatgtggt cgaactaccc gggttaacgg ttatggtgcc
     1081 ggtcgttgaa ccggtcggtg tccaagtgta agtagagccg ccacccgcgg tcagcgtagc
     1141 ggatccgccc tggcagatat ccacatccgg gctgatcgtc gctaccggca ggctgtttac
     1201 cgctacatcc accgtatcgc tgttggtaca accgaatgca tcggttccca caacggtata
     1261 gctggtactg accgatgggt caacagagat ggtcggaccg gtgaatccgc ccggatacca
     1321 tacataacta ttaccgccgc tggcgttc
//
LOCUS       DAOTON010000828         1346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_78669_length_1346_cov_2.237026, whole genome shotgun sequence.
ACCESSION   DAOTON010000828 DAOTON010000000
VERSION     DAOTON010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78669_length_1346_cov_2.237026"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="P5292_13140"
     CDS             complement(<1..531)
                     /locus_tag="P5292_13140"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRS40118.1"
                     /translation="MKRVEDPYSYFRHMDPRSVHKRLLTSYTELLLGSGSPRRRQLLE
                     GIGWPIQVLRKDVPEVPPGHLQGAEVALHLAEFKAMAFDEALAAGQLLLTADTIVWQD
                     GHVLGKPVDRADAVRILDRLQGNRHQVFTGVCLSLLGKRYCFSVETTVTFRPLSSSEI
                     ETYIDEYQPYDKAGAYG"
     gene            546..>1346
                     /locus_tag="P5292_13145"
     CDS             546..>1346
                     /locus_tag="P5292_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS40119.1"
                     /translation="MKNVSLVPGTRCEFVFNRLLFVLLLLPLATAAQDTLVIRLLEDS
                     LQRERNDSLRIKYLNDISWQYHSSDIERAISYADRAGVLAHKSGNQWGIAKSYILHGV
                     YFTIQGQYDSAIANYERCLALRRSLGDSAGVAATYHDIGSVHLFRGDYGPALDFLLRS
                     LRLEQTYGTPQNVAEGFVNVGNVYMAMKRYDDALTNYYQYLKIITPQGDDVAMVEVLN
                     NIGNALLVKERIKEADSSFQVAIELARTKGSLDGEAMSLSGMASVQYKR"
BASE COUNT          302 a          358 c          372 g          314 t
ORIGIN      
        1 tccgtaggcc ccggctttgt cgtagggttg atattcatcg atgtaggttt cgatttccga
       61 actggaaagc ggtcggaagg ttacggtcgt ttctacacta aaacaatacc gcttcccaag
      121 caggctcagg catacgccgg taaacacctg gtgccggtta ccttgcagcc ggtcgaggat
      181 gcgaaccgca tcagcacgat cgaccggctt accgagaaca tgtccgtcct gccagacgat
      241 cgtatcagct gtcagcaata attgaccggc agccagagcc tcgtcaaaag ccatggcttt
      301 gaactcggcc aggtgaaggg ctacttcggc tccttgcagg tgtccgggcg ggacttccgg
      361 aacgtccttt cggaggactt ggatgggcca tccgatccct tccaggagtt gccgcctgcg
      421 aggagatcct gagcccaaca gcagttctgt atagctggtt aacaggcgtt tgtgaacact
      481 ccggggatcc atatggcgaa agtacgaata cggatcttca acccgcttca tgcggcacac
      541 cctgcgtgaa aaatgtatct ttggttccgg gcactcgctg cgaattcgtg ttcaaccgcc
      601 tgcttttcgt tctgctgtta ttgccgctcg ctaccgcggc ccaggatacg cttgtcattc
      661 ggctcctgga ggattccctg caacgggaac ggaatgatag tctccggatc aaatacctga
      721 acgacatttc ctggcaatac cattcgtccg atatcgagcg ggcaatcagt tatgccgatc
      781 gtgcaggcgt cctggcacat aaatccggta accaatgggg aatcgcgaaa tcctacattc
      841 tccacggagt ttattttacc atccaggggc aatacgattc cgccatcgcc aactatgaac
      901 gatgtctggc attgcgccgg tcgctcgggg attccgcagg ggtagctgct acgtatcatg
      961 atatcggcag tgtccacctg tttcgcggcg attatggccc tgccttggac ttcttactgc
     1021 ggtcattgcg gctggagcaa acctatggta cgccgcagaa cgtagcggaa ggtttcgtca
     1081 atgtcggcaa cgtctatatg gccatgaagc gctatgatga cgcgctgacg aattattacc
     1141 agtacctgaa gatcattacg ccgcaaggcg atgacgtggc catggtggag gtgttgaaca
     1201 atatcgggaa tgccttattg gtcaaggagc ggataaagga agccgactcg tcttttcaag
     1261 tggctatcga actcgctcgt acgaaaggaa gtcttgacgg cgaagccatg agcctcagcg
     1321 gaatggccag tgtgcagtac aagcga
//
LOCUS       DAOTON010000829         1343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_78893_length_1343_cov_3.100155, whole genome shotgun sequence.
ACCESSION   DAOTON010000829 DAOTON010000000
VERSION     DAOTON010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78893_length_1343_cov_3.100155"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="P5292_13150"
     CDS             <1..371
                     /locus_tag="P5292_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HRS40120.1"
                     /translation="EKTVLLSTHIMQEVEALCDRVIIINKGRIVADDTTDLLRRKADP
                     EAAIRVSFDRNPTRSQLMQLKGVRDAENMTDTHWRIVPDEGQDVRPELLRYAVENGLT
                     VQSLQVEEQRLEDVFQLLTR"
     gene            375..>1343
                     /locus_tag="P5292_13155"
     CDS             375..>1343
                     /locus_tag="P5292_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40121.1"
                     /translation="MTKKLLAGLLLLIASTAFEAKGQALSAYTDYRGYLQVFDRGMFY
                     QAEYLPVRSYQVGGNAVAYIDNRSDFRIFQNGKANMQVNAADFWYQITNNLIAYKVGN
                     VLYVYDNGQKKILSYYNDGVYLGDSLLAWFDNSNYLLNIYYNGRTAELESSLLEKPKT
                     VKAGPNTLAWVNQSNFFKIFYHGQTYTLDNIPPREFQAGRDIVGYVDDYNGYFHLFYK
                     GDTASVEIFPPDSFKVGWSIAAYVDNLGDFRIFSEGATRRIMPDRPDFFYVVGNVICW
                     GYNGDFNIFWNGNTYVVDRFIPREFKIGPNGVAYLDASNQLKYWYKG"
BASE COUNT          335 a          355 c          353 g          300 t
ORIGIN      
        1 gggaaaagac cgttctgctt tccacgcaca tcatgcagga ggttgaagcg ctctgcgacc
       61 gcgtcatcat catcaacaaa ggccggattg tggccgatga taccaccgac ttactccgcc
      121 ggaaagccga tccggaagcg gcgatccggg tgtcgttcga ccgcaatcct acgcgcagcc
      181 aattgatgca actgaaaggc gtccgggatg ccgagaacat gaccgatacc cattggcgga
      241 tcgttccaga cgaagggcag gatgttcgac ctgaattgct gcgatacgcg gtcgaaaacg
      301 gattgacggt ccaatccttg caggtggaag aacaacggct ggaggatgtg ttccagttac
      361 tcacccgtta gttcatgaca aagaagctac tcgcaggact cctcttgctg attgcctcta
      421 cggcattcga agcgaaaggg caggccctta gcgcttacac cgattaccgg ggttacctgc
      481 aagtctttga ccgcggcatg ttttaccagg cagaatatct gccggtacgt tcctatcagg
      541 tcggcggaaa cgcggtcgcg tatatcgaca accgcagtga cttccgcatt ttccagaatg
      601 gaaaagcgaa tatgcaggtc aacgcggctg acttctggta ccagatcacc aataacctga
      661 tcgcctacaa agtgggcaac gtcttgtatg tctatgacaa cgggcagaag aaaatcctga
      721 gttattacaa cgacggcgtt tacctcggcg acagcctgct ggcctggttc gacaacagca
      781 actacctgct gaatatctac tataacggac gaacggcgga actggaaagc tcactgctgg
      841 aaaagccgaa aacggtcaaa gccgggccca atacgctggc ctgggtcaac cagtcaaact
      901 ttttcaagat cttctatcac ggccaaacct atacactcga caatatccct ccgcgcgaat
      961 tccaggccgg gcgtgatatc gtagggtacg tagatgacta caatggttat ttccacctgt
     1021 tctataaggg ggataccgcc agtgtggaga tatttccacc ggattccttc aaagtggggt
     1081 ggtctattgc cgcgtatgtc gataacctcg gcgactttcg gattttctcc gagggggcga
     1141 cccggaggat catgcccgac cggcccgatt tcttttatgt ggtgggcaat gtcatctgct
     1201 ggggctacaa cggcgatttc aatatttttt ggaatggcaa tacctacgtt gtcgatcgat
     1261 tcatcccccg ggaattcaag atcggaccca acggagtagc ctatctcgat gcgagcaacc
     1321 agcttaaata ttggtacaaa ggc
//
LOCUS       DAOTON010000830         1340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_79167_length_1340_cov_3.250584, whole genome shotgun sequence.
ACCESSION   DAOTON010000830 DAOTON010000000
VERSION     DAOTON010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79167_length_1340_cov_3.250584"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="P5292_13160"
     CDS             complement(<1..351)
                     /locus_tag="P5292_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRS40122.1"
                     /translation="MASIPFNKTKIVATIGPASNTYEVLRDLIRAGVDVCRLNLSHGD
                     YSVHQQVIETIRRINEELRVHTAILIDLQGPKLRIGQVENEPAMLVRGEELVFTSQEC
                     TGNAQQVYMNYRQFP"
     gene            complement(372..794)
                     /locus_tag="P5292_13165"
     CDS             complement(372..794)
                     /locus_tag="P5292_13165"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HRS40123.1"
                     /translation="MKKVVLRIDENEQYGFVLIGIVCQHRDYRLCREINLALDISLAR
                     DNDFEVFTKKRMEAMTFTRFSFTNEEEDEYYMLANKGDGGMLLPEQKQIDYFLLIRPG
                     RNEIDSGNLLPELKKIPMILGAYAFEPKELKSRENLLF"
     gene            complement(856..>1340)
                     /locus_tag="P5292_13170"
     CDS             complement(856..>1340)
                     /locus_tag="P5292_13170"
                     /inference="COORDINATES: protein motif:HMM:NF025973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HRS40124.1"
                     /translation="FREEGFLTEMRSRLVNREQLNKLAVKIGVDRFMQNSIDPGAKNR
                     SAYGDAFEALIGAVYLDQGYNVTRNLVLSRIFRHHIDVDVIEQTDSNFKSKLINWCQK
                     EKRSVDFELMEEVENGGKRLLRVRVLVNGEEVAQGEDFSKKRAEQIAAEKAVGILGIS
                     "
BASE COUNT          264 a          353 c          381 g          342 t
ORIGIN      
        1 cggaaactgt cggtagttca tgtacacctg ctgcgcgtta ccggtacatt cctgtgaggt
       61 gaacacgagt tcttcgccgc gcacgagcat ggccggttcg ttttccacct ggccgatacg
      121 aagtttcggg ccttgcaggt cgatcagaat ggcagtatgg acacgcagtt cttcgttgat
      181 ccgtcggatc gtttcgatca cctgttggtg tacggaatag tcgccgtggg agaggttcag
      241 tctgcagacg tcgacaccgg cgcggatcag gtcgcgcaat acttcatagg tattggatgc
      301 ggggccgatc gtcgcaacga tcttggtctt attgaaagga atggatgcca taaggttgga
      361 tgggaaggtg ctcagaagag caggttctcg cgcgacttga gttctttcgg ttcgaaggcg
      421 taggcgccga ggatcatggg gatttttttc agttccggca agagattgcc ggagtcgatc
      481 tcgttcctac cggggcgaat gaggaggaag taatcgatct gcttctgctc cggcagtaac
      541 attccgccgt cgcctttgtt cgccagcata taatattcgt cctcttcctc gttggtgaag
      601 gaaaagcggg tgaaggtcat cgcttccatg cgctttttgg tgaagacctc gaaatcgtta
      661 tcacgcgcaa gggaaatgtc gagcgccagg tttatttccc ggcagagccg gtagtcgcgg
      721 tgctggcaaa cgattccgat cagcacgaaa ccgtactgtt cattctcgtc gatccgcaac
      781 acgacttttt tcatcccgtg tgaaattacg gaggattatg cgacggtcgt ccgccaagcc
      841 ggaatcttcc tgacttcagg aaatacccag gatgccgacc gctttttcgg cagcgatctg
      901 ttcggcccgc tttttagaaa agtcctcgcc ctgcgccact tcttctccgt tcaccaacac
      961 ccggacgcgt agcaggcgtt tcccgccatt ttccacttct tccatcaatt caaagtcgac
     1021 cgagcgcttc tccttctggc accagttgat gagcttgctt ttgaagttgc tgtcggtttg
     1081 ttcgatgaca tcgacgtcga tatgatgccg gaagatgcgg ctcaggacaa ggttgcgggt
     1141 tacattgtag ccctggtcca ggtaaacggc ccctatcagg gcttcgaacg cgtcgccgta
     1201 agcggagcgg ttcttcgcgc cgggatcgat cgagttctgc atgaagcggt cgacgccgat
     1261 cttcaccgcg agtttgttca actgctcgcg gttgacgagc cggctgcgca tctccgtgag
     1321 aaagccttct tctcgaaagg
//
LOCUS       DAOTON010000831         1339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_79260_length_1339_cov_3.110592, whole genome shotgun sequence.
ACCESSION   DAOTON010000831 DAOTON010000000
VERSION     DAOTON010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79260_length_1339_cov_3.110592"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="P5292_13175"
     CDS             <1..306
                     /locus_tag="P5292_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019942854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40125.1"
                     /translation="SYLPDPWEFIREAQVYRFRFILIDRTACIRGRPTRLTVQYVPET
                     VYPASYPAWLFRETDLKNAFKSDYQLRSVSVSPFSSLRIQEDGAEVEWKSFFFERKR"
     gene            318..>1339
                     /locus_tag="P5292_13180"
     CDS             318..>1339
                     /locus_tag="P5292_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40126.1"
                     /translation="MSAHWNFCTLFNTAYLSRGLALHASLSSVCRSYHLFVFAFDDNC
                     YEYLKNSGLPNLTVISLREFEDEQLLSIKGSRTATEYCWTCTASTILYSIERFGLDHC
                     TYLDADMEFYSDPASLFSEWQHASVMITEHRYTPEYDQSAVSGRYCVQFMAFRNDTRG
                     MQVLRDWRNDCIKWCYARVEDGKFGDQKYLDSWQERYEGVHVLQHPGGGVAPWNVQQY
                     DVSIQDGVLMVTQTTGSSPVPLVFYHYHGLRFYEAGLVMLAGELYEIDGSVVRHLYAP
                     YVGRLLSLGNQVKSLHPSWDPFGVSGRSPKPPLSPWILFLYYFNDLRRSIRNIDGKLI
                     RWRKRH"
BASE COUNT          283 a          323 c          350 g          383 t
ORIGIN      
        1 tcctatctgc cagatccctg ggaattcatc cgggaagcac aagtctaccg ttttcgtttc
       61 atcctgatcg accggacagc ctgtattcgt ggtcggccga cgcgtctgac cgttcagtat
      121 gtgccggaaa cagtttatcc ggccagttat cccgcgtggc ttttccggga gactgatctt
      181 aaaaatgcgt tcaaatcgga ttatcaattg cggagcgttt cagtgtcgcc cttttcatcc
      241 ctgcgtatcc aggaggatgg cgcggaagtg gagtggaagt ccttcttctt tgaacgaaag
      301 cgttaatttg caaagcgatg tcggctcatt ggaacttctg caccttattc aataccgcct
      361 atctgtcacg tgggctggcg ctccacgcat cgttgagtag cgtctgccgg tcttatcatt
      421 tattcgtttt cgcgttcgac gacaactgtt atgagtatct gaagaacagc ggacttccga
      481 atctgacggt catttcgctt cgggagtttg aagatgagca attgttatcc atcaaaggct
      541 cacgtactgc tacggaatat tgctggacct gcacggcctc gacaatactt tattcgatcg
      601 agcggttcgg attggatcat tgtacttatc tggatgctga catggaattc tattccgatc
      661 ctgcttcgtt gttttcggaa tggcagcatg catccgtcat gatcaccgag caccggtata
      721 ctcccgaata cgaccaatcc gccgtgagcg gtcgttattg cgttcagttc atggccttcc
      781 ggaacgatac gcgcggcatg caagtcttgc gtgattggag aaatgattgc atcaagtggt
      841 gttatgcccg tgtggaggat ggaaagttcg gtgatcaaaa gtatctggac tcctggcagg
      901 aacgctacga aggtgtgcat gtattacagc atccgggtgg aggtgtcgct ccctggaatg
      961 ttcagcagta tgatgtctcc atccaggacg gtgttttaat ggttacccaa acgacaggct
     1021 cgtccccggt tccactggtg ttttaccatt accacggact gcgtttttac gaagcaggat
     1081 tggtcatgct tgccggcgag ctgtatgaaa tcgatggatc ggttgttcgt cacctctatg
     1141 cgccttatgt cggtcgtttg ttgtcgttgg gtaatcaggt taaaagcctc catccgtcct
     1201 gggatccctt cggagtgagt ggccggtctc caaagccccc gttgagcccg tggatactct
     1261 ttttgtatta ttttaacgac cttcgtcgct ctattcgtaa tatcgacgga aaactaatca
     1321 ggtggaggaa aaggcatca
//
LOCUS       DAOTON010000832         1333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_79835_length_1333_cov_2.791862, whole genome shotgun sequence.
ACCESSION   DAOTON010000832 DAOTON010000000
VERSION     DAOTON010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79835_length_1333_cov_2.791862"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..339)
                     /gene="nuoK"
                     /locus_tag="P5292_13185"
     CDS             complement(10..339)
                     /gene="nuoK"
                     /locus_tag="P5292_13185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRS40127.1"
                     /translation="MENVTEILRLVPLNHYILLSSLLFSIGVIGVLFRRNAIVILMCV
                     ELMLNAVNLLLAAFSSYHLDPAGQVFVFFIMAVAAAEVAVGLAILTMIYRNIGTVDID
                     ALNKLKW"
     gene            complement(350..847)
                     /locus_tag="P5292_13190"
     CDS             complement(350..847)
                     /locus_tag="P5292_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRS40128.1"
                     /translation="MTLSLFYFLSFLSILSALMVVVSRNPVHSVLYLIITFFAIAGHY
                     ILLNAQFLAAVHVIVYAGAIMVLFLYVIMMLNLNAEVEPHKSAIQKIAATVAGGLLMI
                     VLVGALRNAAVPAPTGTGGPQVGLIHNLGQSLYKDFMLPFEISSILFLAAMVGAVMLG
                     KKEIK"
     gene            complement(864..>1333)
                     /locus_tag="P5292_13195"
     CDS             complement(864..>1333)
                     /locus_tag="P5292_13195"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HRS40129.1"
                     /translation="HLFKKKATIQYPDQQREFAPVYRGQHVLKRDENGAERCTACGLC
                     AVACPAEAITMVAAERQKGEEKLYREEKYAKVYEINMLRCIFCGLCEEACPKEAIFLT
                     DRIVPTEFKRGEFIYGKDKLVEGVDTRVDVSVRQTPSVAAFKQDKSLQHNRLS"
BASE COUNT          298 a          359 c          388 g          288 t
ORIGIN      
        1 ttcactattt caccacttca gcttattcaa cgcatcaata tccaccgtcc cgatattccg
       61 ataaatcatc gtcaggatcg ccaggcctac cgccacttcg gcggcggcaa cggccatgat
      121 gaagaagacg aagacctgac cggccggatc aagatggtag ctggagaatg ccgccagcaa
      181 cagattgacg gcattgagca tcagttccac gcacatgaga atgacgatcg cgttacggcg
      241 aaagagtacg ccgatcacgc cgatgctgaa tagcagacta ctcaacagga tgtaatggtt
      301 caaggggacc aggcggagga tttcggtgac gttttccatg acttcctgct tatttgattt
      361 cttttttacc caacatgaca gctccgacca tggcggcgag gaacaggatg gaactgatct
      421 cgaacggcag catgaagtct ttgtacaacg actggccgag gttgtggatc aatccgacct
      481 gcggaccacc cgtgccagtg ggggctggaa ctgcagcgtt gcgcagcgcg ccgaccagta
      541 cgatcatcag taagcccccg gcgaccgtag ccgcgatctt ctggatcgcc gatttgtgcg
      601 gctccacttc ggcgttcagg ttcagcatca tgatcacata caggaagagt accatgatgg
      661 cgccggcata gacgatcacg tgcaccgcgg ccagaaactg ggcgttgagc aggatgtaat
      721 gtccggcgat ggcgaaaaag gtgatgatca ggtacaacac gctgtgtacc gggttgcggg
      781 agaccaccac catcagcgcg ctgaggatgg agaggaagga gaggaagtaa aagagcgaga
      841 gcgtcatcgc ttagggcttg gggtcaggaa aggcggttgt gctgaaggga tttgtcttgc
      901 ttgaaagcgg cgacggaagg tgtttggcgg accgatacgt ccacccgcgt atccacccct
      961 tcaaccagct tgtcttttcc gtagatgaac tcacctcgtt tgaattcggt cggcacgatt
     1021 cggtcggtga gaaagatggc ttccttcgga caggcttctt cgcagagccc gcagaagatg
     1081 cagcgcagca tgttgatctc gtacaccttc gcgtacttct cttcacggta aagtttctct
     1141 tcgccctttt gacgttcggc cgctaccatg gtgatggctt ctgccggaca agcgaccgcg
     1201 cacaggccgc aggccgtgca acgttcggcc ccgttctcgt cgcgcttcag cacgtgctgt
     1261 ccgcggtaaa ccggggcgaa ctcgcgctgc tggtcgggat attgtatggt cgccttcttt
     1321 ttgaagaggt gcc
//
LOCUS       DAOTON010000833         1331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_79978_length_1331_cov_3.258621, whole genome shotgun sequence.
ACCESSION   DAOTON010000833 DAOTON010000000
VERSION     DAOTON010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79978_length_1331_cov_3.258621"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="P5292_13200"
     CDS             complement(<1..1191)
                     /locus_tag="P5292_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40130.1"
                     /translation="MSKPFIILFLCLQVFASVRAQTVFSKTYGDDAYQNGSALQLLPD
                     GGMVIAGTTGFDQTDSADIALYRMNADGDLLWSVKAGGPVSDYVNNMILTHDQGTILT
                     GQSIVNDPDSAIGALFVIKLDSLGNAEWAQSYDRPGIESGNAIRELANGDFLIAGSTS
                     SPDSMPNGLLLKIDALGNLLWTKRFDLNRSVLFTSLELSPTGSTGEFFVGGTAIDSLH
                     DPNGDFVVLKLDSSGQPLWQFLAGGSGLDVVNALAPLADGGLYGCGTTNSGPSMNEDD
                     GVLFELDSLGNVEWVHHYNRTNDEGFMDLKFDQNRQEVYLCGSITSSGSGPLPLILVT
                     DDAGTVLQSRKQQAVFAEAFCTKLQLTDNGLLLTGQADFADALLVRTDRNGNSPVCQD
                     SIILL"
BASE COUNT          315 a          340 c          369 g          307 t
ORIGIN      
        1 caggagaata atactgtcct gacataccgg gctgtttccg tttcgatcgg tacgtacgag
       61 tagcgcgtcg gcaaaatcgg cttgaccggt cagcagtagg ccattgtcgg tcaattgaag
      121 cttcgtgcaa aatgcttctg caaagacggc ttgttgtttc ctgctctgca gcactgtgcc
      181 ggcgtcgtcg gtgacgagga tcaagggtaa tggaccgctt ccggaagagg tgatgcttcc
      241 gcagagatat acttcctggc ggttttggtc gaatttcaaa tccatgaatc cttcgtcgtt
      301 cgttctgtta taatggtgca cccattcgac gttacccagg ctatccagtt cgaacaagac
      361 accgtcatct tcattcatcg agggaccgga attcgtcgtg ccgcaaccgt agagaccacc
      421 atcggctaag ggcgccaaag cattgacgac atcaagaccg cttccgccag cgaggaattg
      481 ccagagcggt tggccggatg agtcgagttt cagaacgacg aagtctccgt tcggatcgtg
      541 caggctgtcg atggctgtac ctccaacaaa gaactcgccg gttgaaccgg tcggtgacag
      601 ttccagcgag gtaaagagga cggatcggtt cagatcgaat cgtttcgtcc aaagaagatt
      661 gcccaacgcg tcgatcttca aaaggagccc attcggcatt gaatcgggcg aggaagtgct
      721 tccagcgatg aggaaatcac cattcgcaag ctcgcgaatt gcatttccgc tttcaatccc
      781 cgggcgatca taggactgag cccattccgc attgcccaga ctgtcgagtt taattacgaa
      841 aagcgccccg atggcgctgt caggatcatt gacgatcgat tggccggtca ggatagtgcc
      901 ctgatcgtgc gtcaggatca tgttgttcac gtaatcgctc acgggtccac cggctttcac
      961 cgaccacagg aggtcgccgt cggcgttcat tcgataaagc gcgatgtcgg cggaatccgt
     1021 ttgatcaaag ccggtagtgc cggctatgac cataccacca tccggcagta actgcagggc
     1081 gctgccgttt tggtaagcgt cgtcaccgta ggttttggaa aataccgttt gcgcgcgaac
     1141 ggaagcaaac acttgcaggc acaggaaaag gatgatgaac ggctttgaca taacggaaaa
     1201 gtagaaaaga aaacagccgc ccggaggcgg ctgcgaaacg tgttttcaat actcgtcttc
     1261 gttgaagaag aaatcgtctt tggtaggata gtcgggccag atttcttcaa tgctctcgta
     1321 ggcttcgcct t
//
LOCUS       DAOTON010000834         1331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_79994_length_1331_cov_2.554075, whole genome shotgun sequence.
ACCESSION   DAOTON010000834 DAOTON010000000
VERSION     DAOTON010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79994_length_1331_cov_2.554075"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="P5292_13205"
     CDS             <1..719
                     /locus_tag="P5292_13205"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRS40131.1"
                     /translation="TNNNAKMKKIFRFTFLLLFPILTVQAQSSDPTFHFGMKICPNMA
                     WLKSDTKGIESDGSVTRFSYGLITEFRIAENYAFATGIDIAYRGGKLKGETSTVLNDT
                     TTTIVSESTLKLQYIELPLTLKLKTNEIGHLRYYLQVGVAPGFSLRARADGKSTTQIR
                     STGYQANSTAEFEDQDVKDDINNINLSMVIGAGVEYNLSGTTNFFGGIVFNNGFTDVS
                     DSKDAKYVSNFLGLNLGVLF"
     gene            780..>1331
                     /locus_tag="P5292_13210"
     CDS             780..>1331
                     /locus_tag="P5292_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRS40132.1"
                     /translation="MPLRIALAQLDVVIGDFEKNVARMREYLERAKAAGADLVVFPEL
                     AICGYPPRDFLEFRDFIERGHAAAMQLVGDCTDLAAIIGLPTVNPKAEGKNLFNSAWV
                     IQDGKVTATVHKALLPTYDIFDEYRYFEPAREFQTVTVAGKKIALTICEDLWNVEDDP
                     MYVSCPMDALIREAPSPPASGRTT"
BASE COUNT          320 a          403 c          330 g          278 t
ORIGIN      
        1 tgacgaataa caacgcaaag atgaaaaaga tattccgttt cacgtttctt ctcctcttcc
       61 cgatcctcac tgtgcaggca caatcctcgg atccgacatt tcacttcggc atgaagatct
      121 gccccaatat ggcctggctc aagagcgaca cgaaaggcat cgaatccgac gggtctgtaa
      181 cccgattctc ctatggcctg ataaccgagt tccggatcgc cgaaaactat gcattcgcca
      241 ccggtatcga catcgcctat cggggcggta agctgaaagg tgaaaccagt acggtcctga
      301 acgacaccac gaccactatt gtgtccgaat ccaccctgaa gcttcaatac attgaacttc
      361 cccttaccct gaaactgaag acgaatgaaa tcggccacct gcgttactac ctgcaggtag
      421 gtgtcgctcc gggcttcagc cttcgcgcca gggccgacgg caagtcaacc acccaaatcc
      481 gttcgacggg ctatcaggcc aattcgaccg ccgaattcga agaccaggat gtcaaggacg
      541 acatcaacaa catcaacctc agcatggtga tcggtgcagg cgtggaatac aacctgagcg
      601 gcactaccaa tttcttcggc ggaatcgtct tcaacaacgg cttcaccgac gtgtcagatt
      661 caaaggacgc caaatacgtc tccaacttcc tcggcctgaa cctcggcgtg ctgttctgag
      721 gatacccggg aaagtcccta cctttaccgc ctcttccgtt atcgggagag gcgtttttta
      781 tgcccctgcg gattgccctg gcccaactgg atgtcgtcat cggcgacttt gaaaaaaatg
      841 tcgcccgcat gcgcgagtac ctggaacgtg ccaaagccgc cggtgccgat ctcgtagtct
      901 tcccggaact ggcgatctgc ggctatccgc cgcgtgactt tttagagttc cgcgacttca
      961 tcgaacgcgg acatgcagcc gcgatgcaac tcgtcggtga ctgcaccgac cttgccgcga
     1021 tcatcggact gcccaccgtt aatccgaagg cggaaggaaa aaacctcttc aacagcgcct
     1081 gggtgatcca ggatggcaag gtcacggcca ctgtgcacaa agccctattg ccgacctatg
     1141 acatctttga tgaataccgc tacttcgaac cggcccggga attccaaacc gtgactgttg
     1201 cgggtaaaaa gatcgcgctc acgatttgtg aggacctgtg gaacgtggaa gacgacccga
     1261 tgtatgtctc ctgccccatg gatgcgctga tcagggaagc gccttccccg cctgccagcg
     1321 ggagaacaac a
//
LOCUS       DAOTON010000835         1328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_80294_length_1328_cov_2.873527, whole genome shotgun sequence.
ACCESSION   DAOTON010000835 DAOTON010000000
VERSION     DAOTON010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80294_length_1328_cov_2.873527"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1328)
                     /locus_tag="P5292_13215"
     CDS             complement(<1..>1328)
                     /locus_tag="P5292_13215"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRS40133.1"
                     /translation="TTYLSNPKTAWADIDNDGDLDLLLTNHDHTSQIFENDGTGHYTE
                     LTGTGFNTNTITPIESVFEDFDNDGYIDILVTGDEWVYYKNNGDKTFSQVSGLMANNG
                     MLSFAIGDLNHDGFVDVYASYGDIYTNPTNIPDVLYLNNGNSNRFINFQLEGAASNKG
                     AIGGRATIYGDFGVQVREVRAGESYGTCNSSQLHFGLGNVARVDSAVIWFPSGYSTTL
                     YNLESNQFVTVKENSCVIAGNFVTGSSVLCTGQTTTLNAMNGFASYDWSTGDNTQNIQ
                     VSAAGTYNVMVTDNTGCTNISPNFTVQLNPDETPSVTASGELNICQGNAVTLTSSVAV
                     AYNWSNGNSGQSISVDQTGDYSVTIQGVCGSFTSDPVHVEVLPAPAPSATGAAASGPA
                     SVTLNAVGSDLYWYDQQSGGNLLGQGASYVTPVINAPTTYWVEDRVLYPGA"
BASE COUNT          282 a          324 c          417 g          305 t
ORIGIN      
        1 gcacccggat acaggacgcg gtcttccacc caataggtcg taggagcatt gatcaccggc
       61 gtcacgtagg aggcaccctg gccgagcagg ttgccgcccg actgttggtc gtaccaatag
      121 agatcggagc ctacggcatt caaggtaacc gaagcgggac cgctggcagc ggcaccggtt
      181 gctgaaggag caggagccgg gagtacttcg acgtgtaccg gatcgcttgt gaagctgccg
      241 catactccct ggatcgtcac actgtaatcg ccggtctgat cgacggagat ggattgcccc
      301 gagttaccgt tgctccagtt gtaagcgacc gcgaccgagc tggtcagagt caccgcgtta
      361 ccctggcaga tgttcagttc gccggaagcg gtgacggaag gtgtttcatc agggttcagt
      421 tgtacggtga aattcgggct gatgttggtg cagccggtgt tatcggtgac catgacattg
      481 taggtaccag cagcggaaac ctggatgttc tgggtgttgt cgcccgtgga ccagtcgtag
      541 gaggcgaatc cgttcatggc attcagcgta gtcgtctgtc cggtacacag gacggaagag
      601 cccgttacga agttaccggc gatcacgcag gagttttctt tcacggtgac gaactgattc
      661 gattcgaggt tgtacaacgt ggtgctgtaa ccggaaggga accaaatgac agcggagtcg
      721 acacgggcaa cgttgccgag accgaagtgg agttgcgaag aattgcaggt gccgtagctt
      781 tcaccggcgc gcacttcgcg aacctggaca ccgaagtcgc cataaatggt agcacgaccg
      841 ccgattgcac ctttgttcga ggcagcgcct tcgagctgga agttgatgaa gcggttgctg
      901 ttgccgttgt tcaggtacag tacatccgga atgttggtcg ggttggtgta gatgtcgcca
      961 tatgacgcat acacatccac aaaaccgtcg tggttcaggt cgccgatggc gaacgacagc
     1021 ataccgttgt tggccatgag accactgacc tgggagaagg tcttgtcgcc gttgttcttg
     1081 tagtacaccc actcatcgcc ggtaacgagg atgtcgatgt agccgtcgtt atcgaagtct
     1141 tcgaatacgg attcaatcgg ggtgatcgta ttcgtgttga aaccggtacc ggtgagctcg
     1201 gtataatgac cggtgccgtc gttttcaaag atctggctgg tgtggtcgtg gttggtcagc
     1261 aggagatcaa ggtcgccatc gttgtcaatg tcggcccagg ctgttttagg attcgataaa
     1321 taagttgt
//
LOCUS       DAOTON010000836         1327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_80368_length_1327_cov_3.233491, whole genome shotgun sequence.
ACCESSION   DAOTON010000836 DAOTON010000000
VERSION     DAOTON010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80368_length_1327_cov_3.233491"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /gene="ppk1"
                     /locus_tag="P5292_13220"
     CDS             <1..1154
                     /gene="ppk1"
                     /locus_tag="P5292_13220"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRS40134.1"
                     /translation="LFEVIREKDVLLNYPYHSFHPIVDLLREASIDPKVTSIHTTLYG
                     AARYSSVVNALINAAKNGKQVTVVVELQARFDEESNIFYANRLQEEGVRVIFGVPGLK
                     VHSKLFLISRREGGKLAQYAHVGTGNFNEQTARLYCDHALLTSKKPITREVERLFQFY
                     QDNYKTGQYKQLIVSPFHTRKRFIRMIDEEIDNAKKGKEAWMILKMNSLVDKEMIERL
                     YAASEAGVKVKLIVRGICSLVAGEKGLSDNIEAISIVDKFLEHSRIFLFCNGGKEKCY
                     IASGDWMYRNLDFRSEVAVPINDPALREQLKHYVQLQLMDNTKARILDQQQTNRYVRS
                     DSGRKYRAQEEIARWLRQEAEVKKSTTVVEEAPRPAQARSRKKPTLKKK"
BASE COUNT          348 a          340 c          373 g          266 t
ORIGIN      
        1 gcctgttcga agtcatccgg gaaaaggatg tactgttgaa ttatccttat cacagtttcc
       61 acccgatcgt cgacctcttg cgggaagcgt cgatcgatcc caaggtgaca agtatccata
      121 cgacgctgta cggcgccgca cgatacagca gcgtcgtcaa cgccctcatc aatgccgcaa
      181 agaacgggaa acaagtcacg gtggtggtcg agttacaggc gcgcttcgac gaagaatcga
      241 atatcttcta tgccaacaga ctacaggaag aaggcgttcg ggtgatcttc ggtgtgccgg
      301 gactcaaagt gcacagcaaa ttgttcctga tcagccggcg ggaaggcgga aagctggcgc
      361 agtatgcgca cgtcggcacg ggtaacttca acgagcaaac ggccaggttg tactgcgacc
      421 acgcattgct tacctcgaag aaacccatta cccgcgaagt ggaacgattg ttccagttct
      481 atcaggacaa ctacaaaacc gggcagtaca agcagctcat cgtttcgccg ttccataccc
      541 gcaagcggtt catccggatg atcgacgaag agatcgacaa cgcgaagaag ggaaaagagg
      601 cctggatgat cctgaagatg aacagcctgg tcgacaagga aatgatcgaa cggctgtatg
      661 ccgcgagcga ggcgggggtg aaagtgaagc tgatcgttcg aggcatctgt tcgctggttg
      721 ccggcgagaa aggcctcagt gataacatcg aggccatcag catagtcgac aagtttttgg
      781 agcatagccg gatctttttg ttttgtaatg gaggtaaaga gaaatgctac atcgcttccg
      841 gcgactggat gtaccgtaac ctcgatttcc gttccgaagt cgccgtaccg atcaacgatc
      901 ccgccttgcg ggagcaactt aagcattatg tgcagttgca gttgatggac aacaccaagg
      961 ccagaatcct tgaccagcaa cagaccaacc gctatgtgcg cagcgattcc ggccgtaaat
     1021 accgtgcgca ggaagagatt gcgcgatggt tacggcagga agcggaggta aagaaatcca
     1081 caaccgttgt tgaagaagcg ccgcggcctg ctcaagcacg aagccggaaa aagccgactt
     1141 tgaagaagaa ataacttccc ttgcgttacg ccgccatcga tatcggatcc aatgccgttc
     1201 gcctcttact gtgccatgta gagctggagc atggtgagac acactttcga aaaggagaac
     1261 ttgtccgcat tcccttgcgg ctgggagagg atgccttccg cctcggtcgt atcaccgaag
     1321 agcgggc
//
LOCUS       DAOTON010000837         1326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_80495_length_1326_cov_2.380803, whole genome shotgun sequence.
ACCESSION   DAOTON010000837 DAOTON010000000
VERSION     DAOTON010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80495_length_1326_cov_2.380803"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="P5292_13225"
     CDS             complement(<1..1310)
                     /locus_tag="P5292_13225"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS40135.1"
                     /translation="MAAFTSPDRQGCSVPFPVTFNATTPGISGWSWTFGDPGSGSNTA
                     TTQNPSHTYNAFGQYSVTLIVTDANGCKDTLTQVNYVQVIAPTVNFSQTDSGCVGETF
                     NFNAVVTSPADPTIASYQWNWGDGNTSTVTTAGTSHTYNAAGIYDVTLTITTSTGCTA
                     TLTRTQFIRVGTRPTAGITSPIDTICFKETVSFLDASLPPVTGWLWNFGDGGSSTTQN
                     PSHQFNLDTSGVADPFNITLIAFYNGCPDTFVLQDQIVVLGPIPAFTITYNCANPYSV
                     AFTNTSGGATSYSWDFADGSGLSTQTSPTHVFPDNTNYPVTLEATNADNGCVVETTLV
                     AQVREPIANAVVNTQQACYPGTIYFTGSGSQDANQYQWTFGEGIAGVRDTTRFADTLH
                     LYNSVGNYTATLSITDIHGCIDTAQVPIAIVGPDAHFTASPLTD"
BASE COUNT          285 a          314 c          436 g          291 t
ORIGIN      
        1 cagtccgtca atgggcttgc cgtgaaatgc gcgtccgggc ctacaatggc aatcggaacc
       61 tgagccgtgt cgatgcaacc gtgaatgtcg gtaatggaga gcgtcgcggt gtaattgcct
      121 acgctattgt agagatgcag ggtgtcggca aagcgtgtgg tatcacgtac acccgcgatg
      181 ccttctccga aagtccactg gtattggttg gcgtcctgcg agccgcttcc ggtgaaatag
      241 atggtccccg gataacaggc ttgctgagta ttcaccacag cattggcgat cggttcacgg
      301 acctgcgcga ccagggtcgt ttccaccacg catccgttgt cggcgttggt cgcttcgagc
      361 gttacgggat aattggtgtt gtcaggaaaa acgtgggtag ggctggtctg cgtggatagg
      421 ccagaaccgt ccgcgaagtc ccaggagtag gacgtggctc cgccggatgt attggtgaac
      481 gcgacagaat acgggttggc gcaattgtac gtgatcgtga aagcgggaat cggccccagt
      541 acgacgattt ggtcctggag gacaaacgta tcgggacagc cgttataaaa tgcgatcagg
      601 gtgatgttga aaggatcggc gactccggaa gtgtccaggt tgaactgatg gctggggttc
      661 tgcgtggtac tggaaccacc atcaccgaag ttccacaacc agccggtaac aggtggcagg
      721 gaggcgtcga ggaaagagac cgtttccttg aagcaaatgg tatcgatcgg agaagtaatt
      781 cctgctgtcg ggcgggtacc gacacggatg aactgcgttc tggtcaaggt ggccgtacag
      841 ccggtggagg tggtgatcgt cagtgtgaca tcgtagatgc ccgctgcatt ataggtatgg
      901 gaagtaccgg cggtggtgac ggtggacgta ttgccatccc cccaattcca ttggtagctt
      961 gcgatcgtgg gatcagccgg tgaggtgacc actgcattga agttgaacgt ctcaccgacg
     1021 catccgctgt cggtctggga aaagtttacg gtcggtgcga tcacctgaac gtaattgacc
     1081 tgggtcaatg tatccttaca gccattcgcg tcggtcacga taagggtgac ggaatattgc
     1141 ccgaacgcgt tgtaggtatg actggggttt tgcgtggtag cggtattgga accactgcct
     1201 ggatcgccaa acgtccagct ccaaccggaa atcccgggcg tggtagcgtt gaatgtaacg
     1261 gggaagggaa cgctgcatcc ctggcgatcg ggagaagtga acgctgccac cggcggtggg
     1321 ttgacc
//
LOCUS       DAOTON010000838         1324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_80703_length_1324_cov_2.486998, whole genome shotgun sequence.
ACCESSION   DAOTON010000838 DAOTON010000000
VERSION     DAOTON010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80703_length_1324_cov_2.486998"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="P5292_13230"
     CDS             complement(<1..1029)
                     /locus_tag="P5292_13230"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680290.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRS40136.1"
                     /translation="MSHEFPTRAENYSQWYQDLVIKADLAENSAVRGCMVIKPYGYAI
                     WEKMQAVLDQMFKDTGHVNAYFPLFIPKSFFSKEASHVEGFAKECAVVTHYRLKNAED
                     GSGVVVDPEARLEEELIVRPTSETIIWNTYRGWIQSYRDLPILVNQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATAEEAIIETEKMLDVYAEFAEQYLALPVIKGVKTPNERFA
                     GALETYCIEAMMQDGKALQAGTSHFLGQNFAKAFEVKFTNKEGKQDYVWATSWGVSTR
                     LMGALIMAHSDDDGLVLPPKLAPVQVVIVPIYKTDEQLKAISAVADQLAAEFKAKGIR
                     VKYDDRDTH"
     gene            1110..>1324
                     /locus_tag="P5292_13235"
     CDS             1110..>1324
                     /locus_tag="P5292_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40137.1"
                     /translation="MAGTFAGERSKAMKTIRFRYILPVIALITSCSSTYYPGSTPADD
                     VYYSPRNNPAPAPVATAPAPALNTYSN"
BASE COUNT          282 a          406 c          332 g          304 t
ORIGIN      
        1 gtgcgtgtcg cgatcgtcat acttgacgcg gataccctta gccttgaact cggccgctag
       61 ttggtcggca accgcggaga tcgctttcaa ctgctcgtcc gtcttataga tcggcacgat
      121 caccacctgg accggcgcca gcttgggtgg caagaccagt ccgtcatcgt ccgaatgcgc
      181 catgatcaac gcgcccatca gacgggtgga aacaccccag cttgtggccc agacataatc
      241 ttgtttacct tccttgttgg tgaacttcac ctcaaaggcc ttggcgaagt tctgtccgag
      301 gaaatgtgag gtgccggcct gaagcgcctt accatcctgc atcatcgctt cgatgcaata
      361 agtctccaac gccccggcga aacgctcgtt cggggtcttg actcccttga tgaccggtaa
      421 cgcgaggtat tgctcggcaa actcggcgta gacgtcaagc atcttttcgg tttcgataat
      481 ggcctcttcg gccgtcgcgt gcgcggtatg accttcctgc cacaggaact cagccgttcg
      541 caggaacagg cgtgtacgca tctcccaacg caccacgtta gcccactggt tcaccaggat
      601 aggcagatcg cggtacgact ggatccatcc ccggtaggta ttccagatga tggtctcgga
      661 ggtaggccgc acgatcagtt cctcttccaa tcgcgcttcc ggatccacca ccacgccgct
      721 tccatcttcc gcgttcttga gccggtaatg ggtgaccacg gcacactctt tggcaaagcc
      781 ttccacatgg gaagcttctt tggaaaagaa cgatttcgga atgaacaagg ggaaataagc
      841 gttcacgtgg ccggtatcct tgaacatctg gtccagtact gcctgcatct tctcccagat
      901 cgcgtacccg taaggcttga tgaccataca gccccgaacg gccgaatttt cggccaggtc
      961 ggctttgatg accaggtcct ggtaccactg cgaataattc tctgcccggg tggggaattc
     1021 gtgactcata gttaaacttc gaactgttgg tccgttttgg tacggtgttt gtaaaattca
     1081 aacaaaagta accaatttta ccctcactca tggccggcac cttcgccggt gaaaggagca
     1141 aagcgatgaa aacgattcga ttccggtaca tcctcccagt gatcgccttg atcacttcgt
     1201 gcagctcgac ctactatccc ggcagcactc cagccgacga tgtgtactac tcgccgcgca
     1261 acaacccggc gcccgctcct gttgctaccg ctcccgcgcc ggcactcaac acgtactcca
     1321 acaa
//
LOCUS       DAOTON010000839         1323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_80791_length_1323_cov_2.774448, whole genome shotgun sequence.
ACCESSION   DAOTON010000839 DAOTON010000000
VERSION     DAOTON010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80791_length_1323_cov_2.774448"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="P5292_13240"
     CDS             <1..967
                     /locus_tag="P5292_13240"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRS40138.1"
                     /translation="EAEFAGYCSVQHTVGVANGLDAMILALRSYNFSPGDEVLVPSNT
                     YIASILAILHAGLKPVLVEPRLSTYNINPENIREKISKRTRAVLVVHLYGKLCEMDAI
                     LSIAREYGLAVVEDCAQAHGASLRGKRAGSFGDFGAFSFYPTKNLGALADAGAVTTDD
                     PKKAEAIRTLRNYGSEKKYYNSIVGYNSRLDELQAAFLRVKLAALDKINAHKRKLAAI
                     YQAELSDAYVKPVKQEDYLDVFHIYAIRHPKRDQLRTFLSENAIHTEVHYPVAPNRQL
                     AMRGILDMTDTPIAQEIHETILSLPISFFHTEDDIRQVVGVMNRF"
     gene            969..>1323
                     /locus_tag="P5292_13245"
     CDS             969..>1323
                     /locus_tag="P5292_13245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS40139.1"
                     /translation="MKRLSVITVNLNNARGLRKTLQSVITQSWSDREIIVIDGGSSDE
                     SLAVIEEFRSHLSFVHSGKDAGVYDAQNKGIALASGEYCLFLNSGDYLASPDVLSAVF
                     SRRRDEDILYGNMIIY"
BASE COUNT          307 a          325 c          363 g          328 t
ORIGIN      
        1 cgaggcggag ttcgcaggct attgcagtgt gcagcatacg gtaggcgtcg cgaatggatt
       61 ggatgctatg atccttgcct tgcgttccta taatttttct ccgggagatg aggttctggt
      121 tccatcgaat acgtacatcg catccatact ggcgattctg catgcgggct tgaagccggt
      181 attggtcgaa ccccgcttgt ccacctacaa tatcaatccg gaaaacatcc gcgaaaagat
      241 cagcaagcgt acccgtgccg tgttggtggt acacctctac gggaagttgt gtgaaatgga
      301 tgccatcctt tccatcgccc gggagtatgg tctggccgta gttgaagatt gtgcccaggc
      361 ccatggagcg agcttacgtg ggaaacgggc cgggagcttc ggtgatttcg gggcctttag
      421 tttttatccg accaagaacc tcggagcact cgccgatgcc ggagccgtca cgaccgatga
      481 tccgaagaaa gcggaagcga tccgcacact tcggaattac ggttctgaaa agaagtatta
      541 taacagcata gtcggttaca attcgcggtt ggatgagctg caggcggctt ttctacgcgt
      601 gaaactcgct gcattggaca agatcaatgc gcataaacgc aaactggctg ccatctatca
      661 ggcggaattg tccgatgctt atgttaagcc cgtgaagcag gaggattacc tggatgtctt
      721 tcatatttat gctattcgac atcccaagcg tgaccagctg cggacctttc tgtcggagaa
      781 cgctatacat accgaagtgc attatccggt cgctcccaac cggcaattgg caatgcgcgg
      841 gatcctggat atgaccgata ccccgatcgc acaggaaata cacgaaacca tactcagcct
      901 gccgatctct ttcttccaca cggaagatga cattcggcag gttgtcgggg tgatgaaccg
      961 cttttgacat gaagcgtctt tcggtcataa cggtcaacct gaacaatgcc cgtggtttgc
     1021 ggaaaacgtt gcaaagcgtg atcacccagt cgtggagcga tcgtgaaatc atcgtgatcg
     1081 atggcggctc gagtgatgaa agtttggcgg tgatcgaaga gttccgttcg cacttgtctt
     1141 tcgtccacag tgggaaagac gctggcgtct acgatgcaca gaacaagggg atcgcactcg
     1201 cctccggcga atattgtctt tttctgaatt cgggtgatta tctggcctca cccgatgttt
     1261 taagcgcggt tttcagccgt cgacgggatg aggatatctt gtacggcaac atgatcattt
     1321 att
//
LOCUS       DAOTON010000840         1320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_81068_length_1320_cov_2.173123, whole genome shotgun sequence.
ACCESSION   DAOTON010000840 DAOTON010000000
VERSION     DAOTON010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81068_length_1320_cov_2.173123"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1320
                     /locus_tag="P5292_13250"
     CDS             <1..>1320
                     /locus_tag="P5292_13250"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRS40140.1"
                     /translation="LTATVTGGGEPYTYDWSDGTGSIGATNPLTVNPATSSVYTVTIT
                     DACLNTTTATVSVSVLPSPTVSLSGPAGICTSGPATLTASGTAISYAWPGNLDPTVYT
                     TTVYFSPSAQPIETNVGPGNVVASTTLPALPAGAVVNSYTLTYNGITALTNSFGSDVN
                     LGFQGAVTAAATSGIGSPAAAGTFNFVQTYTGGVNTAGGTVDLLYWDVADDNTGTAEA
                     TFPTGTNVASLVISYQVPASGTTLSVTPTATTTYTVIGTGASGCTATATWTVVIPPAS
                     TTTATATPANVCVNGTSQLNAVASYPPPSAPTYCAVTNQGSSCVTSVSINTLNSTPPA
                     CVTPFYHFNAPTGSNTTTLMPGSTYTFNVTTGGTAIVSVFIDFNRNGVYEASEWFQPY
                     TAATSGSISIPVPSGASVGQTGMRVRSRLNGNQNDATTACLVGMGSG"
BASE COUNT          248 a          447 c          345 g          280 t
ORIGIN      
        1 cgctgaccgc aacggtcacc ggtggtggcg aaccgtacac gtacgactgg agtgatggta
       61 ccggttcgat cggtgcgacc aatccgttga cggttaaccc ggccacatcg tccgtttata
      121 cggtaacgat cacggacgct tgtctgaaca cgaccactgc aactgtttct gtcagcgtat
      181 tgccttctcc gacggtcagt ctctccggtc ctgccggaat ctgtacctcc ggtcctgcaa
      241 ccctgaccgc ctccggcacc gccatcagct atgcatggcc tggcaatctg gatccgacgg
      301 tttatacgac cacggtctat ttcagcccgt cagctcagcc gattgaaacc aacgttggtc
      361 ctggtaacgt agttgccagc accaccttgc cggcacttcc tgccggtgct gtggtgaact
      421 cctacacgct gacctacaac ggtatcaccg ccctgaccaa ctccttcggt tctgacgtga
      481 atctgggctt ccagggcgca gtgaccgcgg ccgctacgag cggtatcggc tcaccggctg
      541 ctgcaggcac cttcaacttt gttcaaacct acacaggcgg tgtgaatacc gccggtggta
      601 cggtcgacct cctgtactgg gatgtcgctg acgacaatac cggaactgcg gaagctacct
      661 tcccgaccgg aaccaacgtg gccagcctgg taatttccta ccaggtgccc gccagcggaa
      721 ccaccctgtc ggtaaccccg accgctacga ccacctatac ggtgatcggt accggcgctt
      781 cgggttgtac cgccacggcg acctggacgg tagtcattcc tccggcctct acgacgacgg
      841 ctacggctac tccggccaac gtctgcgtga acggtaccag ccagctcaat gcagtggctt
      901 cctacccgcc gccttcggct ccgacctatt gtgcagtaac caaccagggc agctcgtgcg
      961 tgaccagcgt gtctatcaat acgctgaaca gcacgcctcc ggcttgtgta acgcccttct
     1021 accacttcaa cgctccgacc ggttcgaaca ccacgaccct gatgccgggc tccacctaca
     1081 cgttcaacgt gaccacgggt ggtaccgcga tcgtgtccgt gttcatcgac ttcaaccgca
     1141 acggtgtgta cgaagcttcc gaatggttcc agccttacac ggcagcgacc tccggctcga
     1201 tttccatccc ggtaccttcc ggtgcctcgg ttggtcagac cggtatgcgc gtacgttccc
     1261 gcctcaacgg caaccagaac gatgcaacca ccgcttgttt ggtgggtatg ggctccggtt
//
LOCUS       DAOTON010000841         1317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_81307_length_1317_cov_3.057845, whole genome shotgun sequence.
ACCESSION   DAOTON010000841 DAOTON010000000
VERSION     DAOTON010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81307_length_1317_cov_3.057845"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1317
                     /locus_tag="P5292_13255"
     CDS             <1..>1317
                     /locus_tag="P5292_13255"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRS40141.1"
                     /translation="PMTFNDEVKRYINMYAVQKRGLTERVMGLANLYFPLYEQILDQQ
                     GLPLEFKYLSIVESALNPMAVSPVGATGLWQFMLPTGRLYGLKVNSLIDERRDPVKST
                     YAACQYFKDMYAIYNDWLLVIAAYNCGAGNVNRAITRSGGKHSFWEISPYLPRETRGY
                     VPAFIAVTYLMNYTGEHNLTAVPPVVNFFDIDTVLVDQKVALRDISDAIDIPYDLLAY
                     LNPVYKRGIIPDAEEGQPLRLPVNKVNTYLGSLDRIYKPQETPVASALFASNEASGDD
                     GADYVSETVRKYHKVRRGDNLGSIAGKYNCTVSDLKRWNRLKSTRLMAGQNLAVYVSV
                     KKKSPVMAARVKPAPQPAGTDTAAPANDSAQATASTASVNTTASHNGPSIPTPDNMKV
                     VYHIVQPGDTLWNIASRYEGVTVERIKEMNALRDNEIKVGTKLKVV"
BASE COUNT          297 a          433 c          347 g          240 t
ORIGIN      
        1 cccgatgacc ttcaatgacg aggtcaagcg ttacatcaac atgtacgctg ttcaaaagcg
       61 cggtctgacc gaacgcgtga tgggactggc caacctgtat ttcccgcttt acgaacagat
      121 cctcgaccag cagggacttc cgctcgaatt caagtacctc tccatcgtgg agagcgcgct
      181 caacccgatg gcggtttctc cggtcggcgc gacgggcctt tggcaattca tgctgccgac
      241 cggtcgcttg tacggtctca aggtcaactc cctcatcgac gaacgacgcg atccggtgaa
      301 gtcgacctat gccgcctgtc aatatttcaa ggacatgtac gcgatctaca acgactggct
      361 gttggtcatc gccgcgtaca actgcggagc cggcaacgtc aaccgcgcca tcacccgctc
      421 gggtggcaag cattcgttct gggagatcag tccatacctg ccgcgtgaaa cacgcggtta
      481 tgtaccggcc ttcatcgccg tcacttacct gatgaactac accggcgaac acaacctcac
      541 cgcggttcct ccggtggtca atttcttcga catcgacacg gtgttggtcg accagaaggt
      601 cgccctgcgt gatatctccg acgccatcga catcccgtac gacctcctcg cgtacctcaa
      661 cccggtgtac aaacgcggta tcatccccga tgccgaggaa ggacagccgc tgcgtcttcc
      721 ggtgaacaag gtgaacacct acctcggcag cctcgatcgc atttacaagc cgcaagagac
      781 cccggtagca tccgccctgt ttgcttcgaa cgaagcgtcc ggcgacgatg gagcggatta
      841 tgtcagtgag accgtcagga agtaccacaa agtgcgtcgc ggcgacaacc tcggcagcat
      901 cgccgggaag tacaattgca ccgtatccga cctcaagcgc tggaaccgcc tgaagagcac
      961 ccgcctgatg gccggacaaa acctcgccgt gtacgtcagc gtgaagaaga aatcgccggt
     1021 gatggcggcg cgcgtcaagc cggcgccaca gcctgccggt accgacacgg ccgctcctgc
     1081 gaacgattcc gcgcaagcca cggcctccac cgcctcggtt aacaccacgg cttcccataa
     1141 cggaccctcg atcccgacgc cggacaacat gaaggtggtt taccacatcg ttcaacccgg
     1201 cgacaccctc tggaatatcg cctcacgcta cgaaggcgtg accgtcgagc gcatcaagga
     1261 gatgaacgcc ctccgcgaca acgagatcaa agtaggcacc aagctcaaag tggtcat
//
LOCUS       DAOTON010000842         1317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_81320_length_1317_cov_2.633122, whole genome shotgun sequence.
ACCESSION   DAOTON010000842 DAOTON010000000
VERSION     DAOTON010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81320_length_1317_cov_2.633122"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1317)
                     /locus_tag="P5292_13260"
     CDS             complement(<1..>1317)
                     /locus_tag="P5292_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40142.1"
                     /translation="NWISGIKFLDEARLGPQDNLPSTLAKNRGRNTYYLLPLLLGLVG
                     LVYHYLRNPKDTVVVFWLFFMTGIAIVLYLNQKPLEPRERDYSYAGSFYAFAIWIGLG
                     VLALYEQLRKFLPSVAGASVSLALCTLAVPVIMAEENWDDHDRSGRYTARDFAINYLQ
                     SCDSNAVVFTNGDNDTFPLWYVQEVEGIRTDVRVVNLSYLGADWYIDQMKRKVYDSDP
                     LPIQFTSDQFRQGRRDIIYIVERTQDKLPLKEALYDWLGSDDPRTQSIPGYQGRFDYL
                     PAKNYTLAVDTNAVRNSGILTKRELPSMVSSVDIKIDKNYITKSDMLVLDMLATSNWK
                     RPLYFAVTVSRDNWLGLDPYLRLEGLAYRILPVRGSTDIARPGTVAADIMYDNMMNKF
                     RWGGIDNPHVYLDENNQRMLLNMRNNFARLAEALLAEGKNDSARKVL"
BASE COUNT          344 a          304 c          327 g          342 t
ORIGIN      
        1 aaggacttta cgggctgaat cgtttttccc ttcggccaga agcgcttcgg caagccgggc
       61 gaaattgttc cgcatattga gcagcattct ctggttgttt tcatcgaggt acacatgggg
      121 gttatcgatg ccaccccaac ggaatttgtt catcatattg tcgtacataa tatcggcggc
      181 aacggttccg ggacgtgcaa tatccgtaga tcccctgaca ggaagaatcc ggtaggcaag
      241 gccttcgagc ctgagatagg gatcaagtcc gagccagttg tcgcgtgaca ccgttactgc
      301 aaaatacaaa ggccttttcc agttgctggt agccagcata tccagaacca gcatatcgct
      361 tttagtgata tagtttttgt caattttgat gtcaaccgag gaaaccatgg acggtaattc
      421 acgttttgtt agaattccag agtttctgac ggcatttgta tcgacagcca gagtatagtt
      481 tttggcaggc aaataatcaa atctgccctg gtatccggga atggactggg tgcgcgggtc
      541 atcgcttcca agccaatcgt aaagagcttc tttcagtgga agtttatcct gggtacgttc
      601 cactatgtag atgatatctc ttcggccctg ccggaactga tcgctggtga actgaatagg
      661 aaggggatct gagtcataga ctttccgttt catctgatca atgtaccagt cagcgcccag
      721 atagctcaga ttcacaacgc ggacatccgt tcttattcct tctacttcct gaacgtacca
      781 gagaggaaag gtgtcgttgt cgccattggt aaagaccaca gcgtttgaat cgcacgactg
      841 gagatagttg atggcgaaat cccgggccgt gtaccgaccg gaacgatcat gatcatccca
      901 gttttcttcg gccattatta ccggtacagc aagggtacat agggcaagac ttacagacgc
      961 tccggccact gaagggagaa atttacggag ttgttcataa agcgccagaa cacccaatcc
     1021 tatccagata gcgaaggcgt aaaaagaacc tgcgtaggaa taatcccttt ctcttggttc
     1081 aagcggtttc tggttcagat ataatacgat ggctataccg gtcatgaaga agagccagaa
     1141 tacgaccact gtatctttgg ggtttctgag gtaatgatat accaatccaa ccaaacccaa
     1201 aagcaaagga aggagatagt aagtatttct tcctctgttt ttggcaaggg tagatggcag
     1261 attgtcctgc gggcccaggc gggcttcatc cagaaacttg attcccgaaa tccagtt
//
LOCUS       DAOTON010000843         1317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_81323_length_1317_cov_2.492868, whole genome shotgun sequence.
ACCESSION   DAOTON010000843 DAOTON010000000
VERSION     DAOTON010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81323_length_1317_cov_2.492868"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1317
                     /locus_tag="P5292_13265"
     CDS             <1..>1317
                     /locus_tag="P5292_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40143.1"
                     /translation="PTDIRIRFDGIDRLKVKEDGSLLFETSVGSVEFPAPVVYQQING
                     ARKTIAAAYRVRENNMLSFELGEYDVNAPMIIDPIALRWATWVNTASSGDNHGHCIWV
                     DPSDGAIYMVARVVGTTDQITIGAFDELANGNLEMIVGKYLEPVNIGEAGTRVWQTYI
                     GGNNDDNPYAMEQGPDGNLYITGYTGSTNFPLLGGTGFSGSSIDNQSQSGNDIFILKI
                     NRNGTGIKASVLGGNGDEGSYDLRINEAGDVVICGQTTSTNLSTLYGLGASNTNNGSN
                     DWLVFKINQDLSALAWMKNNGGSGSDIATIMVQNVANGDLFIGGSTASTNFPTLNPRQ
                     STRGGSSAGFIQKMNAVGNLQWASYFSSATSQSASILCMEFNTSKNQLYFGGLTTGLN
                     AANISASGVIDNSVNGGNDFFVCRMDTNQTFVAGTYLGGSGTEENM"
BASE COUNT          326 a          387 c          345 g          259 t
ORIGIN      
        1 cccgacggac atccgcattc gattcgacgg cattgatcgt ctcaaggtga aagaagacgg
       61 tagcctcctg ttcgagacgt cagtcggttc ggtagaattc cccgcaccag tcgtgtacca
      121 gcagatcaac ggggcccgta aaaccatcgc agccgcgtat cgcgtacgcg aaaacaacat
      181 gctgtccttc gaacttggcg aatacgacgt aaatgcaccc atgatcatcg acccgatcgc
      241 cttgcgttgg gcaacctggg tgaacacagc ctcctccggc gataaccacg gtcactgtat
      301 ctgggtggat ccttccgacg gcgcgatcta tatggtcgct cgtgttgtgg gcacaactga
      361 tcagattacg atcggagcgt tcgacgagct agcgaatggt aacctggaaa tgatcgtagg
      421 caaatacctc gaacccgtca acattggcga ggcaggcaca cgggtatggc agacctacat
      481 cggcggtaat aacgacgaca acccctacgc gatggagcag ggcccggacg gcaacctcta
      541 catcaccggc tataccggca gtacgaactt cccgctgctt ggcggtaccg gattcagtgg
      601 ttccagcatc gataatcaaa gccaatccgg taacgacatt ttcattctca aaatcaaccg
      661 taacggaacc ggaatcaagg catcggtact gggcggaaac ggcgacgaag gttcctatga
      721 cctgcgcatc aatgaagcgg gcgatgtggt gatctgcgga caaaccacca gcactaacct
      781 ctcgaccttg tatggccttg gcgccagcaa cacgaacaac ggcagcaacg actggctggt
      841 cttcaagatc aaccaggacc tttccgcttt ggcgtggatg aagaacaacg gtggcagcgg
      901 ttccgacatc gcgacgatca tggtgcagaa cgttgccaac ggcgatctct tcatcggcgg
      961 ctcaaccgca tctaccaatt tcccgaccct gaacccgcgt cagagcacgc gcggtggcag
     1021 cagcgcaggc ttcattcaga agatgaacgc agtgggtaat cttcaatggg catcctattt
     1081 ctcctccgcc accagccaga gcgcctccat cctttgcatg gagttcaaca cctcaaagaa
     1141 ccaactgtac ttcggcggtc ttaccaccgg tctgaacgca gccaacatct cggcatccgg
     1201 tgtcatcgac aacagtgtga acggcggaaa cgactttttt gtttgtcgca tggacaccaa
     1261 ccagacgttc gtcgccggaa cctacctcgg tggatccggt acagaggaga acatgat
//
LOCUS       DAOTON010000844         1314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_81594_length_1314_cov_4.183479, whole genome shotgun sequence.
ACCESSION   DAOTON010000844 DAOTON010000000
VERSION     DAOTON010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81594_length_1314_cov_4.183479"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="P5292_13270"
     CDS             <1..1016
                     /locus_tag="P5292_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRS40144.1"
                     /translation="VRMARMHADSAVTLLESLGDLNALSNALETQSKIEAMAGNFSAA
                     YRQQVRFKMLNDSIFNMERDRKFAQTTLQYEFEKKEAAAKAEQEKKDIRQRNIRNSIT
                     AGLGGALIFLVVVYRQRNRISKEKKRSDELLLNILPEEVAEELKAKGEADAVQIEQAT
                     VLFTDFKGFTAMSESLSPKELVRDLNECFSAFDRITEKYGIEKIKTIGDSYMAAGGLP
                     TPNSTHAADVVRAALEMRDFIAAGKSKKLSAGHPYFEVRIGVHTGPVVAGIVGVKKFQ
                     YDIWGDTVNTASRMESSGEAGKVNVSSTTYALVKDRFACSHRGKISAKGKGEVDMYFV
                     EPM"
     gene            1025..>1314
                     /locus_tag="P5292_13275"
     CDS             1025..>1314
                     /locus_tag="P5292_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40145.1"
                     /translation="MTDRAKLRLIKLVHTAIWIFFNLVIGYMLYAVIVGKIDRRLWIC
                     YGLIVGEGLTLLLFNMFCPLTVWARRYSDSTKANFDIYLPEWLAKYNKEIYTT"
BASE COUNT          327 a          336 c          385 g          266 t
ORIGIN      
        1 acgtcaggat ggcccgcatg catgccgaca gtgccgttac tttactggaa tccctgggcg
       61 acctcaacgc actgtcgaat gcacttgaga cccagagtaa gatcgaggcg atggccggca
      121 acttcagcgc cgcgtatcga cagcaggtac gtttcaagat gctcaacgat tccatcttca
      181 acatggaacg cgaccgcaag ttcgctcaga cgacccttca gtacgagttt gaaaagaagg
      241 aagcggccgc gaaagctgag caggagaaga aggatatccg gcaacggaac atccggaatt
      301 ccataacggc aggcctgggc ggcgcgctca tcttcctggt cgtggtctac cggcagcgta
      361 accggatcag caaggagaaa aaacgaagcg acgagttgct cctcaacatt cttcctgaag
      421 aagtggcgga ggaactgaag gcgaaaggcg aagcggatgc cgtgcagatt gaacaggcga
      481 ccgtactctt caccgatttc aagggcttca ccgcgatgag tgaatcgctc agtccgaagg
      541 aactggtacg tgatctcaac gagtgcttca gcgcgttcga ccggatcacc gaaaagtacg
      601 ggatcgagaa gatcaagacc atcggcgact cctacatggc tgccggtgga ttgcccacgc
      661 ccaattcgac gcatgccgcg gatgtagtca gggccgcgct ggagatgcga gacttcatcg
      721 cagcgggtaa atcaaagaaa ctgtccgccg gccatccgta tttcgaagtg cgcatcggcg
      781 tgcataccgg cccggtcgtg gcagggattg tcggcgtgaa aaaattccaa tacgacatct
      841 ggggcgacac ggtcaacacc gccagtcgga tggagagcag cggcgaagcg ggaaaggtca
      901 atgtttccag tacgacctat gcattagtca aggaccgttt cgcttgcagt catcgtggaa
      961 agatcagcgc gaaaggcaaa ggcgaggtcg acatgtactt cgtggaaccg atgtgattcc
     1021 aacgatgacg gaccgagcca aacttcggct gatcaagctg gtccataccg cgatttggat
     1081 tttcttcaac ctggtcatcg ggtacatgct gtatgccgtg attgtcggca agatcgatcg
     1141 gcggctctgg atctgttatg gcttgatcgt tggcgaagga ttgaccttgt tgttgttcaa
     1201 catgttttgt ccgctaacgg tttgggcgcg gcggtattcc gattcgacga aagcgaactt
     1261 cgacatttac ctgcccgaat ggctggcgaa gtataacaag gagatctata cgac
//
LOCUS       DAOTON010000845         1314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_81619_length_1314_cov_2.824464, whole genome shotgun sequence.
ACCESSION   DAOTON010000845 DAOTON010000000
VERSION     DAOTON010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81619_length_1314_cov_2.824464"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="P5292_13280"
     CDS             <1..664
                     /locus_tag="P5292_13280"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRS40146.1"
                     /translation="RQLSEAILAFQIDCAFLNIEDRVVGMTYSEFGRRVKSNASFGTD
                     HGAAAPVLVFGKQVQSGIVGASPVIPYQAGSNNNLPMQYDFRSVYASILQDWFCVPPT
                     DLQTILFNNYQTLPIIKSSACLGIGIHELNQAAGLQLISNSPNPFSSSTYITYTTQGG
                     HTRVQVFSPEGKLIKNLVDAEQSAGTYKVWFENEHFAPGVYYARLQNGPIAQVANMVI
                     VP"
     gene            complement(665..1129)
                     /locus_tag="P5292_13285"
     CDS             complement(665..1129)
                     /locus_tag="P5292_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRS40147.1"
                     /translation="MSVHRFSITQLLPLSLEEAWEFFSTPRNLARITPPEMNFRIVTP
                     DLPDETYAGQIIAYTLTPVAGIRSGWITEITHVKAPHFFVDEQRKGPYRMWHHQHHFR
                     AHKSGTEMTDIVHYEIPLGKLGDALNALYIRNRIHGIFEHRRKVLEQLFPQK"
     gene            1215..>1314
                     /locus_tag="P5292_13290"
     CDS             1215..>1314
                     /locus_tag="P5292_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRS40148.1"
                     /translation="MTARAEAFERLATIMDELRAQCPWDKKQTLESL"
BASE COUNT          279 a          376 c          375 g          284 t
ORIGIN      
        1 gcgtcagcta tcggaagcga tcctggcttt ccagatcgat tgcgcgttcc tcaacatcga
       61 agatcgtgtg gtcggaatga cctattccga attcggacgt cgcgtcaagt cgaacgccag
      121 cttcggaacc gaccacggcg cggccgcacc ggtgctggta ttcggcaagc aggtgcaaag
      181 cggcatcgtg ggtgcgagtc cggtcatccc gtaccaggca ggatcgaaca acaacctgcc
      241 gatgcaatac gacttccggt ccgtgtacgc gtccattctc caggattggt tctgcgtgcc
      301 tccaactgat ctgcagacga tcctgttcaa caattaccag acgttaccga tcatcaagtc
      361 gtcggcctgc ctgggcatcg gcatacacga gttgaatcag gcggcagggt tgcaactgat
      421 atccaattca ccgaatccct tctcgtccag tacctacatc acctacacca cgcaaggcgg
      481 tcatacccgc gtgcaggtgt tttcgccgga aggtaagctg atcaagaacc tggtggatgc
      541 ggagcaatcc gccggcacct acaaggtgtg gttcgagaac gagcatttcg cgccgggtgt
      601 ttactacgcc cgtttgcaga atggccccat cgcccaggtg gcgaacatgg tcatcgtgcc
      661 ctgatcattt ttgcgggaag agttgctcca gcacctttct acggtgctcg aaaatgccgt
      721 ggatcctgtt ccggatgtag agtgcgttga gcgcatcacc cagctttcca agcggaatct
      781 cgtagtgcac gatatcggtc atttcggtgc cgcttttgtg cgcacgaaaa tgatgctggt
      841 ggtgccacat ccggtaggga ccctttcgtt gttcatccac gaagaagtgc ggcgccttga
      901 catgcgtgat ctccgtgatc caaccggagc ggatacccgc gaccggcgtc agggtatagg
      961 cgatgatctg tccggcgtac gtttcgtccg gcaggtcggg cgtgacgatg cggaagttca
     1021 tttccggcgg tgtgatccgg gccaggttgc gcggagtcga gaaaaattcc caagcctctt
     1081 ccagcgaaag cggcagcaat tgggtgatgc tgaatcggtg tacactcatg tcggaaaccc
     1141 taacaagcgg cgcctaaaaa ggttccgggg atgcagtatc ttgtccccgt taccaaagcc
     1201 gcttgaaatc cgctatgacc gcacgcgccg aggccttcga acgactggcc accatcatgg
     1261 acgagcttcg cgctcaatgt ccctgggaca agaaacagac gctggaatcg ctgc
//
LOCUS       DAOTON010000846         1313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_81756_length_1313_cov_2.240064, whole genome shotgun sequence.
ACCESSION   DAOTON010000846 DAOTON010000000
VERSION     DAOTON010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81756_length_1313_cov_2.240064"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="P5292_13295"
     CDS             complement(<1..313)
                     /locus_tag="P5292_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40149.1"
                     /translation="MSIRGIFSIIALFLFQSLACSAQADQAAIPSHTRTYHCLIRGDS
                     TKHFTVRYAIIQSVTDSGFYYSAVKHGSRSFEREVRFMPARYIDEMKIGRKGKTGTGI
                     LV"
     gene            complement(375..>1313)
                     /locus_tag="P5292_13300"
     CDS             complement(375..>1313)
                     /locus_tag="P5292_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40150.1"
                     /translation="STLIAGRYTIYLETAAGTSPVHDFSLPDPDQYIRDFDIVAPDRW
                     FTLVGSRYIGGPTTLWRSVDRGNSWQVDSSFCAAERNTDPSPNYWNSVNQLQHFGTDT
                     LALFLGYYQSGVAYSIDGGASWTNWMQNLITHYHAIFQCDTTYYLFGLEGDAFRSWAF
                     AFPRYKLFRNDSLVNFQVFGGTGDHPFCSNGDSSCFFVPGSLNRCGQYHYMRDYIDSL
                     CQLTTGIDAVNFSDADGLFVFPNPGNGLFGIRFPGDWVGVANVTINDLSGRTVRSVRL
                     PVSDNTTLDLGSLPAGTYLLRLDNNGDFRSGKLLKY"
BASE COUNT          332 a          329 c          384 g          268 t
ORIGIN      
        1 ccacgaggat gccggttcct gttttcccct tccggccgat cttcatttcg tcgatatagc
       61 gcgcgggcat gaaccggact tcccgttcaa aagaacgcga gccgtgtttg accgcggaat
      121 agtagaaccc gctgtcggtg accgattgga tgatcgcgta gcgaacggtg aagtgcttgg
      181 ttgaatcacc gcggatgagg cagtgatagg tgcgggtatg ggaaggaata gctgcctggt
      241 ctgcttgggc gctacaagca agtgactgga acaggaagag tgcgatgatt gaaaaaatgc
      301 cgcgaattga catggttggg agattttgga atagtcaacg atcatacagc tgatacactg
      361 gtcctggcga tcggttaata cttaagaagt tttccggacc tgaaatctcc gttattgtcc
      421 aggcgcagca aataggtccc ggccggaagg gagccaagat caagggtggt gttgtccgaa
      481 accggaagac gaacggatcg taccgttcga cctgacagat cgttgatggt cacattggct
      541 actccaaccc agtcgccggg aaatcggatc ccgaataaac cgttgccggg attcggaaac
      601 acgaacagcc cgtcagcatc ggagaaattc accgcgtcga ttccggtcgt tagctggcaa
      661 aggctgtcga tgtagtcgcg catgtagtga tactgtccgc aacggttcaa actgccgggc
      721 acgaaaaaac aactgctgtc gccattcgag cagaagggat gatcgcccgt accgccgaag
      781 acctggaagt tgacaagcga atcgttccgg aagagtttat aacgcgggaa agcaaaggcc
      841 cacgaacgaa aggcgtcgcc ttccagcccg aagaggtagt aggtcgtgtc gcactggaag
      901 atggcgtggt aatgcgtgat caggttttgc atccagttgg tccaactggc acctccatca
      961 atggaataag ccactcccga ctggtaataa cccagaaaca gggccaatgt atccgtaccg
     1021 aaatgctgca actggttgac cgagttccaa taattcggcg aagggtctgt attcctttcg
     1081 gctgcacaga aagaagaatc cacctgccag gaattcccgc gatcaaccga gcgccaaagc
     1141 gtcgtcggac cgccgatgta tcggctgccg acgagcgtga accaacggtc cggcgctacg
     1201 atatcgaagt cgcggatgta ttgatccggg tccggcagac tgaaatcgtg taccggactg
     1261 gtaccggcag cggtctccag gtaaattgta taccgaccgg caatgagcgt cga
//
LOCUS       DAOTON010000847         1312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_81819_length_1312_cov_3.106603, whole genome shotgun sequence.
ACCESSION   DAOTON010000847 DAOTON010000000
VERSION     DAOTON010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81819_length_1312_cov_3.106603"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..707)
                     /gene="rpsD"
                     /locus_tag="P5292_13305"
     CDS             complement(102..707)
                     /gene="rpsD"
                     /locus_tag="P5292_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRS40151.1"
                     /translation="MARYTGPKSKVSRRFREPIFGPDKALERKNYAPGQHGQTKKRAK
                     QSEYAVQLQEKQKVKFLYGILERQFAKIFDRASRRTGITGENLLQLMETRLDNVVYRL
                     GIAPTRRAARQLVGHRHITVNGKVVNIASFNCKAGDVVAVREKSKALEAITGAVTGHV
                     NKHSWLDWDKSTMSGKVLNLPARDQIPENIKEQLIVELYSK"
     gene            complement(753..1160)
                     /gene="rpsK"
                     /locus_tag="P5292_13310"
     CDS             complement(753..1160)
                     /gene="rpsK"
                     /locus_tag="P5292_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRS40152.1"
                     /translation="MATQTNAAKAAKTAKKKVVKVEATGRAYVTATFNNIIISLTNES
                     GQVISWSSAGKMGFKGSKKNTPYAAQMAAADASKVAHDMGLRKVKVFVRGPGAGREAA
                     MRSLHQNGIEVTEIMDITPIPHNGCRAPGRRRV"
     gene            complement(1170..>1312)
                     /gene="rpsM"
                     /locus_tag="P5292_13315"
     CDS             complement(1170..>1312)
                     /gene="rpsM"
                     /locus_tag="P5292_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRS40153.1"
                     /translation="LVDINSYRGMRHRNGLPVRGQRTRTNCRTRKGKRKTIANKKKAS
                     KM"
BASE COUNT          230 a          353 c          386 g          343 t
ORIGIN      
        1 tcactttgtc cggtttctga aacgcgagga tggccattgt atcgttgggg agttaagtat
       61 tgtttgattg aacaggttgg atcggatgcc cgatcgtcag attacttcga gtacaattcg
      121 acgatgagct gttccttgat gttctccggg atctggtcgc gtgccggcag gttcaacacc
      181 ttgccgctca tcgtcgactt gtcccagtca agccaggaat gcttgttcac gtgacccgtc
      241 acagctccgg taatggcttc cagcgccttc gacttctcgc gaaccgcgac gacgtcgccg
      301 gccttgcagt tgaacgaagc gatgttgacc actttaccgt tgacggtgat gtgacggtgt
      361 ccgaccaact ggcgagcggc ccgacgggtc ggggcgatac ccaggcggta tacgacgttg
      421 tcaagacggg tctccatcaa ctggagcagg ttctcacccg tgatgcccgt acggcgggat
      481 gcgcgatcga agatcttcgc gaactggcgc tcgaggatgc catagaggaa tttaaccttt
      541 tgcttctcct ggagctgaac tgcgtattcc gattgcttcg cacgcttctt ggtctggccg
      601 tgctggccgg gagcgtaatt cttgcgttcg agtgccttgt ccgggccgaa gatcggctcg
      661 cggaatcgac gggatacttt ggatttgggt ccggtataac gtgccatgaa tctttgttgt
      721 caggtgttag aggattgtac ttccggtcgg gtttagacgc gacgacggcc gggagcgcga
      781 catccgttgt gagggatcgg ggtgatatcc atgatctcgg tcacctcaat tccattctgg
      841 tggaggctac gcatcgctgc ttcacgtcct gcacccggtc cgcgaacgaa cactttcact
      901 ttgcggagac ccatgtcgtg ggccaccttc gaagcgtccg ctgctgccat ctgggcggcg
      961 tacggcgtgt tcttcttgga tcctttgaag cccatcttgc cggcggaact ccaggaaatc
     1021 acctggcccg actcgttggt caacgagatg atgatgttgt tgaaggtagc ggtcacatag
     1081 gcgcgacccg tggcctccac tttcaccact tttttcttgg ccgtcttggc ggccttcgct
     1141 gcgttggttt gcgttgccat gatgatcaat tacattttag atgccttctt cttgttggca
     1201 atggtcttac gtttaccctt acgggtacga cagttggtac gggtgcgctg accacgaacg
     1261 ggcagaccgt tccggtgccg catgccgcgg tagctgttga tatccaccag ac
//
LOCUS       DAOTON010000848         1302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_82808_length_1302_cov_3.291099, whole genome shotgun sequence.
ACCESSION   DAOTON010000848 DAOTON010000000
VERSION     DAOTON010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82808_length_1302_cov_3.291099"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..>1302)
                     /gene="icd"
                     /locus_tag="P5292_13320"
     CDS             complement(111..>1302)
                     /gene="icd"
                     /locus_tag="P5292_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRS40154.1"
                     /translation="KAYQGKKKIVWKEVLAGEKAFNQTGNWLPDETLAAFKEYLVGIK
                     GPLTTPVGGGIRSLNVALRQILDLYVCLRPVRWFEGVPSPVKNPGAVDMVIFRENTED
                     IYAGIEFVAGSPEAKKVLDFLAKEFPKEFNKIRFGAEEKGAEYLRMAGSNDSAEVNVG
                     LGLKAVSRIGSERLIGSAIDYAIRHKRKSVTIVHKGNIMKFTEGAFRDWGYALAEKKY
                     GDKVYTWSQWERTKKEKGEAAANDEQKAALASGRVLVKDAIADITLQQVLTRPEDFDV
                     IATLNLNGDYLSDALAAQVGGIGIAPGANINYVTGHAIFEATHGTAPKYANQDKVNPG
                     SVILSGAMMLEHLGWIEAANLIYSGMEKSIGAKRVTYDFERLMQGSTLLKCSEFGDEI
                     IRNM"
BASE COUNT          248 a          367 c          369 g          318 t
ORIGIN      
        1 ggccaaaggt aatcattgag ggggtacggc agcggccgga tcggggtgaa aatgaacaac
       61 ccctgcgggt acgattcgac cggcaggggt tgcgttgaga aaaagttcgt ttacatgttc
      121 cggatgatct cgtcgccgaa ctccgagcac ttcaacaggg ttgatccctg catgagacgt
      181 tcgaaatcat acgtgacgcg tttcgcgccg atactcttct ccataccgga atagatcagg
      241 ttggccgctt cgatccagcc gaggtgctcc agcatcatgg cacccgagag gatcacggag
      301 ccggggttca ctttgtcctg gttggcatac ttgggcgcgg ttccgtgggt agcttcgaag
      361 atggcgtggc cggtaacata attgatgttc gcgcccgggg cgatgccgat accgcccact
      421 tgtgcggcga gcgcatcgct caggtagtcg ccgttcaggt tcagggtcgc gatgacgtca
      481 aagtcttccg gtctcgtcag gacctgctgc agggtgatgt ctgcgatcgc gtccttcacc
      541 aatacacggc cggaagccag cgcggctttc tgttcgtcat tagcggctgc ttcgcctttc
      601 tctttcttcg tgcgctccca ttgcgaccag gtgtaaacct tatcgccgta ctttttctcg
      661 gcaagtgcat aaccccaatc gcggaaagcg ccttcggtga acttcatgat attccctttg
      721 tgaacgattg tcaccgactt ccgcttgtgc cggatcgcgt agtcgatcgc gctgccgatg
      781 agccgctcgg agccgatcct ggaaacggct ttcaggccca ggcctacatt cacttcggct
      841 gaatcattcg aacctgccat gcgcagatat tccgcgcctt tttcttcagc tccgaagcgg
      901 atcttgttga actctttcgg gaattctttc gccaagaagt cgagcacttt tttcgcttcc
      961 ggtgagccgg caacgaattc gatacccgcg tagatgtctt cggtgttttc gcggaagatg
     1021 accatgtcca ccgcgccagg gttcttgacg ggtgacggta cgccttcgaa ccaacgaaca
     1081 gggcggaggc acacgtaaag gtcaaggatc tggcgaagcg ccacgttgag cgaacggatg
     1141 ccgccgccaa caggtgtggt taacgggcct ttgatgccga ccaggtattc cttgaacgca
     1201 gccagtgttt cgtcggggag ccagttcccg gtctgattga acgccttttc gccggccagg
     1261 acttctttcc aaacaatctt tttcttgccc tgataggctt tc
//
LOCUS       DAOTON010000849         1297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_83372_length_1297_cov_4.251208, whole genome shotgun sequence.
ACCESSION   DAOTON010000849 DAOTON010000000
VERSION     DAOTON010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83372_length_1297_cov_4.251208"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="P5292_13325"
     CDS             <1..533
                     /locus_tag="P5292_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRS40155.1"
                     /translation="TMKKPLLFTLALALSFCFGYAFKTLVDPVPKEPSTFKRVTGIGG
                     IFFKCKDPAALRAWYQTHLGLQTNAYGAVFEWWQGADSSKKGFTQWSPFKETTKYFEP
                     STKEFMINYRVADMERLVDALRKEGVTITDSIQAFDYGKFVHILDLEGNKIELWEPND
                     LVYEQMGVQMGSPTTK"
     gene            complement(677..>1297)
                     /locus_tag="P5292_13330"
     CDS             complement(677..>1297)
                     /locus_tag="P5292_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40156.1"
                     /translation="EVYGLGTGWDQVNAANTQVGHPFYGLGDPIIGFRHKHVLLSMRG
                     GAWGTPGVLPATLLPGTQYRQTYSLAVPEEYWGSSGQINTIDSASISLVGLVQQYSAN
                     SFDRRILQADAGKLTDLLTGTGSPNASPMFLVGPSPARDYLTVRSLRGTIARVFLFDL
                     AGRPVFSAASGEGSLRINTTELAAGSYILLVQGSDQQAMQYRVVIE"
BASE COUNT          301 a          388 c          355 g          253 t
ORIGIN      
        1 caacgatgaa aaagcccctc ctctttaccc tggccctcgc gctttcgttc tgtttcggct
       61 atgcctttaa gacattggtc gatccggtgc cgaaagaacc ttcgaccttc aaacgcgtca
      121 ccggcatcgg cgggatcttt ttcaagtgca aggaccccgc cgcgctgcgc gcctggtatc
      181 agacccacct cggactgcaa accaacgcct atggcgccgt cttcgaatgg tggcagggcg
      241 cagattcgag caagaagggc ttcacacagt ggagcccgtt caaggagacg acgaagtact
      301 tcgagccctc gacgaaagag ttcatgatca actaccgggt cgccgacatg gaacggctcg
      361 tcgacgcgct gcgaaaggaa ggcgtcacga tcaccgattc catccaggcc ttcgattacg
      421 gcaagttcgt acacatcctc gacctcgaag gcaataagat cgagctctgg gaaccgaacg
      481 acctcgtgta cgaacaaatg ggcgtccaga tgggcagccc gacgacgaag tgagactccg
      541 gaggtttatg accactggga catagcgatc aacgcagtgc tactttttca aaatcaccac
      601 aaattgtctg atcgctgaaa accatccgcc ggacgcaatg aactcgaatg atcgaacacg
      661 acgggcctga tgccggtcat tctatgacta ctcgatactg catcgcctgc tgatcgctgc
      721 cctgaaccaa taggatgtag ctgcccgccg cgagttcggt agtattgatc cggaggctgc
      781 cttcgcccga cgccgcggag aataccggtc gtccagcaag gtcaaacagg aacacgcgtg
      841 cgatcgtgcc ccgcaaggaa cgcaccgtca ggtaatcccg ggccggcgaa ggtcccacaa
      901 ggaacatcgg cgaggcgtta ggactgcctg ttccggtaag caggtctgtc agtttgccgg
      961 catccgcctg gagaatgcgt cggtcgaagg aattagcgct gtattgctga accaatccga
     1021 ccaggctgat tgaagccgaa tcaatcgtat tgatctggcc gctggatccc caatattcct
     1081 ctggcacagc gagcgagtag gtctgtcgat actgtgtacc aggcaggagc gtcgcgggta
     1141 gtacacccgg agtaccccat gcgccgccgc gcatcgacaa caagacatgc ttgtgtcgaa
     1201 atccgatgat cggatcaccg agtccgtaaa acggatgtcc aacctgcgtg ttcgccgcgt
     1261 tcacctggtc ccagccggtg cccagtccgt acacttc
//
LOCUS       DAOTON010000850         1296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_83487_length_1296_cov_3.072522, whole genome shotgun sequence.
ACCESSION   DAOTON010000850 DAOTON010000000
VERSION     DAOTON010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83487_length_1296_cov_3.072522"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="P5292_13335"
     CDS             complement(<1..210)
                     /locus_tag="P5292_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HRS40157.1"
                     /translation="MVFSSALFLLYFLPLFLLVYHIVGERWKNGVILLFSLFFYSWGA
                     PRFVFVLLALTGIDFFIVRKMYGTTD"
     gene            complement(266..925)
                     /locus_tag="P5292_13340"
     CDS             complement(266..925)
                     /locus_tag="P5292_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HRS40158.1"
                     /translation="MLDKNGIAKRIAREVKDGMYVNLGIGIPTLVANYIPAGVEVVLQ
                     SENGLLGIGPFPFEGEEDPDLINAGKQTVTTLKGSSFFDSAMSFGMIRAGKVDLTILG
                     AMEVSEQGDIANWKIPGKMVKGMGGAMDLVASAKHIIVAMQHVNKAGESKLLPECSLP
                     LTGRRCVKKIVTELAVLDVLSEGGFKLLERAPGVSVEEIVRSTAGKLIVEGEIPEMKL
                     D"
     gene            complement(1001..>1296)
                     /locus_tag="P5292_13345"
     CDS             complement(1001..>1296)
                     /locus_tag="P5292_13345"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRS40159.1"
                     /translation="FNGKEYLLEYAFDAPFAIVKAWKGDTDGNLIFRETARNFNPLMA
                     MAGKLTIAEVEELVPAGELDPDHIHTPGIYVHRIFQGPVYEKRIEQRTVRKRG"
BASE COUNT          285 a          358 c          357 g          296 t
ORIGIN      
        1 atccgttgtt ccgtacatct tgcgcacgat gaaaaaatcg atgcctgtca acgccagaag
       61 cacgaagacg aagcggggtg cgccccagct ataaaaaaag aggctgaaaa gcaggataac
      121 accgttcttc caccgttcac caacaatgtg ataaaccagg agaaacaagg gcaggaagta
      181 cagcagaaag agtgcgctgc tgaaaaccat ctataggcga atattgggga aagaaaaggc
      241 cgaggttggt gccccggccg tttggtcaat ccagtttcat ttccgggatc tcgccctcga
      301 caatcaactt gccggcggtc gagcgtacga tctcttcgac gcttaccccc ggagcgcgtt
      361 cgagtagttt gaagcctcct tccgacagta cgtccaatac ggccagttcg gtgacgatct
      421 tcttgacgca cctgcgaccg gtgaggggaa gtgaacattc cggcagcaac ttcgattcgc
      481 cggccttgtt gacgtgttgc atggccacga tgatgtgctt ggcggaagcg accaggtcca
      541 tcgccccgcc cattcccttc accatcttac ccgggatctt ccagttggcg atgtcgccct
      601 gttcgcttac ttccatggcg cctaagatgg tgagatcgac ctttccggcg cggatcatgc
      661 cgaaactcat ggcggaatcg aagaacgagg agcctttcag cgtcgtaacg gtctgcttgc
      721 cggcattgat cagatcagga tcttcttcgc cttcgaacgg aaaaggtccg ataccgagca
      781 atccattttc agattgcaac actacttcca cgccggcggg aatgtaattg gcaaccaggg
      841 tagggatccc gatgccgagg ttgacgtaca tcccgtcctt tacttcccgg gcgatgcgtt
      901 tggcgattcc gttcttatct aacatggctc gcgatgattg actttagaaa cttgtggcaa
      961 ttccaattac tcatctgggg aaatcggcta agaccttcga tcagccccgc ttccggaccg
     1021 tccgctgttc gatgcgtttc tcgtacacgg gaccctggaa gatgcgatgg acgtagatgc
     1081 ccggcgtgtg gatgtgatcg ggatccagtt cgcccgcggg aacgagttct tccacttccg
     1141 cgatggtcag cttgccggcc atcgccatga gcggattgaa gttgcgggcg gtctcccgga
     1201 agatcaggtt gccgtcggta tcgcctttcc aggctttcac aatggcgaag ggcgcgtcga
     1261 acgcgtactc cagcaggtat tcttttccgt tgaagg
//
LOCUS       DAOTON010000851         1295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_83619_length_1295_cov_2.707258, whole genome shotgun sequence.
ACCESSION   DAOTON010000851 DAOTON010000000
VERSION     DAOTON010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83619_length_1295_cov_2.707258"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1295
                     /locus_tag="P5292_13350"
     CDS             <1..>1295
                     /locus_tag="P5292_13350"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40160.1"
                     /translation="VAAQSGKVLAGGNFTRGSFRERYFIAGLRHSTKSVLDWKPTVNS
                     AVYDLSVAGNRLYLAGGFSEVESVPRWGSAAYDFPSGTLSNWDVSFGNGTLYSIFASG
                     DKVYAGGTFNMAGISERNSVACVDTVTGSALSWNPSANGNIRHINKSGNSMLLGGEHR
                     FLKGAPRTHLAAIDSASGLLLEDFSPNPNANVNDLLVTNGKLYVGGFFSNIGGAYHPF
                     LADLDFTYGNYQGLIAAPNAGVRDLALDSIGNIYLGGDFNQVDGTPVSYLAKLNAAGI
                     LQTWNPDPDNGVYCIEPTTNSISVGGAFTQFTSSSTSRPYFGQLDYSGNPTSYNPAPN
                     GSPICFHVNGNTFYTGGYFYTFDGQSRTHLSAIDLNTSTLLPFAPQLNGGVATLASYG
                     NLLFAGGDFNYIDTLNAPFIGAISTSTGFGVPFNVTLDG"
BASE COUNT          297 a          355 c          308 g          335 t
ORIGIN      
        1 gtgtcgccgc gcaatccggc aaggttctcg cgggaggaaa tttcacgcgt ggttccttcc
       61 gggaacgtta cttcattgca ggcctgcgac acagtacgaa atccgtactc gactggaaac
      121 ccacagtcaa tagcgcggtc tatgacctgt cagtagcagg aaatcgactt tacctggcag
      181 gcggtttcag cgaagtggaa agtgtcccgc gctgggggtc cgcggcctac gactttccat
      241 ccggtaccct ttccaactgg gatgtgtcat tcggtaatgg gaccttatac tccatcttcg
      301 cttccggtga taaagtctat gcaggcggca cctttaatat ggccggcatc tccgagcgta
      361 actcagtagc ctgtgttgac acggtaacag gttctgcgct ctcctggaat ccttcagcaa
      421 acgggaatat tcggcacatc aacaaatccg gaaattccat gcttctgggc ggcgagcatc
      481 gtttcctcaa aggagctccc cggacccatc ttgctgctat cgactccgcc agtggtcttc
      541 tactggaaga cttcagtccg aatcccaacg cgaatgtcaa cgatttactg gtcacgaacg
      601 ggaaattata tgtcggagga ttcttctcga acataggagg tgcctaccat ccttttcttg
      661 ccgatcttga ttttacctac ggcaattatc agggcttgat cgccgcaccg aatgcaggtg
      721 tccgagacct tgcgttggat tctatcggta acatttacct gggtggcgac ttcaaccaag
      781 tcgatggcac accggtttcg tatttggcta aactcaacgc ggcgggaatt cttcaaacct
      841 ggaaccccga tccggataat ggtgtctatt gcattgaacc gaccacaaac tccatttccg
      901 taggcggagc gtttacccaa ttcacttctt cgagtacctc gcgtccttat tttggacaat
      961 tggattatag cggcaatcca accagctaca acccggcacc gaatggaagc ccaatctgct
     1021 tccacgttaa cggaaatacg ttttataccg gcgggtactt ttacactttc gacggacaat
     1081 ccagaaccca tttatccgca atcgatctaa acacttctac ccttctaccc tttgcaccgc
     1141 agttgaacgg cggggtggca acacttgcat cctatggcaa tttgttgttc gcaggcggcg
     1201 atttcaatta tatcgacacg ctcaatgctc cctttattgg tgcgatcagc acgagtacag
     1261 gattcggtgt tcctttcaat gtgacattgg acgga
//
LOCUS       DAOTON010000852         1293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_83829_length_1293_cov_2.819063, whole genome shotgun sequence.
ACCESSION   DAOTON010000852 DAOTON010000000
VERSION     DAOTON010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83829_length_1293_cov_2.819063"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="P5292_13355"
     CDS             <1..314
                     /locus_tag="P5292_13355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40161.1"
                     /translation="IDDLQITGTLVNVDEVDETTLGYALYPNPTSAASHVQFKLNKNS
                     AVSLQVKDLTGRLVREVLNSDLGAGVHEFDIPTDGSGIYLIDLSVNGKHHIRRLVVTQ
                     D"
     gene            445..1125
                     /locus_tag="P5292_13360"
     CDS             445..1125
                     /locus_tag="P5292_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40162.1"
                     /translation="MRIQRLALFLLLLLSAGNPTSAQEVPEYKKNAFQFNLGNYGVNE
                     INFGFEHFFSARRSLEINGGLVYRNDFLVDMAKDWTNSLYFYERGFTVRAQYKLFKKK
                     PEDSKWRDYISPMIYFKYLYYGKTWFANELKNEKTGDPYDEYIYQTRFRDRFGFQFHF
                     GKIYEMNQTFALEFYYGVGLRGTLVNRIDVAKQDSANAPVYQVNWQDDRFYVRPSIHG
                     GVKLRVSF"
BASE COUNT          293 a          370 c          324 g          306 t
ORIGIN      
        1 acatcgatga tctccagatc accggcacgc tggtgaatgt ggatgaagta gacgagacca
       61 ccttgggtta tgcgctttat ccgaacccga cctccgctgc cagccatgtt cagttcaagt
      121 tgaacaagaa cagcgccgtc agcctgcagg tgaaagacct caccggccga ctggtacgcg
      181 aagtgctgaa ctccgacctt ggagccggcg tgcatgagtt cgacattcct accgacggat
      241 cgggcattta cctgatcgat ctttcggtga atggcaaaca tcatatccgc cgcctggtgg
      301 ttacgcagga ttgatctgcc ttctatcatg atgaaagcgg ctgcccttac cggcagccgc
      361 ttttttgttt aaaacgaagt ttggatatcg catcagggcc tttaaaaacc gatccttttt
      421 ccgatttttg ccgtagaaca acctatgcgc atccagcggc tcgccctttt cctcctgcta
      481 ctactttcgg ccggcaaccc tacctccgcg caggaggttc cggaatataa aaagaacgcg
      541 ttccagttca acctcggcaa ctacggggtg aacgagatca acttcggctt cgagcacttt
      601 ttctccgcgc ggcgtagtct tgagatcaac ggcggactgg tctatcggaa cgatttcctg
      661 gtcgacatgg cgaaagactg gaccaactcg ctttacttct acgaacgcgg tttcacggtc
      721 cgggcacagt acaaactctt caagaagaaa cccgaagatt ccaaatggcg cgactacatc
      781 tcgcccatga tttatttcaa gtatctgtat tacgggaaaa cctggttcgc gaacgaattg
      841 aaaaacgaaa agaccggcga tccctatgac gagtacatct accagacccg cttccgcgac
      901 cgcttcggat tccagtttca cttcggcaag atctacgaga tgaaccagac gttcgcgctg
      961 gagttctact acggcgtcgg cctgcgcggc acgctggtca accggatcga cgtcgccaaa
     1021 caagactccg cgaacgcccc ggtgtaccag gtcaactggc aggacgaccg cttttatgtg
     1081 cggccgtcga ttcacggcgg tgtaaagctg cgcgtttcct tctaacccgt taccgagaag
     1141 atttgaggag gcattgtgtt tgtcaatgcg cttccagcca gttcttaccc gaaccgattc
     1201 ccacttcgat cggaactttc agtttgaggg cgtgcttcat gcggtcctcc acgagttccc
     1261 tgacggtgtc gagttcttcc cggtggcaat cga
//
LOCUS       DAOTON010000853         1292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_83953_length_1292_cov_2.872272, whole genome shotgun sequence.
ACCESSION   DAOTON010000853 DAOTON010000000
VERSION     DAOTON010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83953_length_1292_cov_2.872272"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="P5292_13365"
     CDS             complement(<1..227)
                     /locus_tag="P5292_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40163.1"
                     /translation="MSSRKKNLSKSGNKQAPHAPALPPLAPGLFYTHRFAFQALFLFV
                     LGFLLYANSIPNEYALDDGLVIKENIWVQQGT"
     gene            complement(312..1178)
                     /locus_tag="P5292_13370"
     CDS             complement(312..1178)
                     /locus_tag="P5292_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HRS40164.1"
                     /translation="MQAVFRNYLSLVKFSHTVFALPFAVIGYFLGISRPGYSFDFLLF
                     LKVLACMVTARTAAMAFNRLIDRRIDAQNERTRSREIPAGTISPVAATALVAVSCLLF
                     ILTTWTINPLCFYLSPVALSVVLGYSYTKRFTWLCHLVLGLGLSLAPIGAYLAVTGVF
                     DFLPICFSLCVITWVSGFDIIYALQDEAFDRSLSLYSMPASFGKKKALGISSLLHMLS
                     AGFVILAGYLGDFGWLYWIGVTLFIALLIYQHSLVKPDDLRRVNLAFFTTNGIASLVF
                     AIFVVGELLLHH"
BASE COUNT          350 a          312 c          380 g          250 t
ORIGIN      
        1 gtgccctgct ggacccagat attttccttg atgaccagtc cgtcgtccaa agcgtattcg
       61 tttggaatac tgttggcata gagcaggaac ccgagcacga aaaggaaaag tgcctgaaaa
      121 gcgaaccggt gggtatagaa cagtccgggt gccaggggag gcagggcagg cgcatgcgga
      181 gcctgtttgt tccctgattt ggataagttc tttttacggg aagacactgc gatcggaatt
      241 ggtcgtttga ccaaagtacg aaggcagcgc agattgcgca aggcattcgc tgtgccggca
      301 atgcgcttaa atcagtggtg gagcagcaat tcgcccacta cgaagattgc gaaaaccagg
      361 ctggcgattc cgtttgtcgt gaaaaaggcc aggttcaccc tgcgcaggtc gtcgggcttt
      421 accaggctgt gctgatagat caacaaggca ataaatagcg tcaccccgat ccagtacagc
      481 caaccgaagt cacccagata gcccgcgagg atgacgaacc cggcagaaag catgtgtaac
      541 aacgaagaga ttcccagggc ttttttctta ccaaaggatg ccggcatcga gtagagtgaa
      601 agcgatcggt caaacgcttc gtcctgcaat gcatagatga tgtcgaaccc gcttacccag
      661 gtgatgacgc acagggaaaa acagatcggt aaaaagtcga acacaccggt aacggcaaga
      721 taagctccga tcggtgcgag tgacaagcca agtccgagga ccagatggca cagccaggtg
      781 aagcgtttgg tgtacgaata acccaatact accgacaagg caacagggct gaggtagaaa
      841 cacaacggat taatcgtcca ggtggtcagg atgaagagga ggcaactaac ggcgaccagc
      901 gcggtagctg ctacaggcga aatggttccc gccggtattt ccctggatct ggtacgttcg
      961 ttttgagcat cgatcctgcg gtcgatcaga cggttgaatg ccatcgcggc ggtccgcgct
     1021 gttaccatac acgccagaac ttttagaaac aacaggaagt cgaacgagta gccagggcgg
     1081 gagatgccca ggaaatatcc gataaccgca aagggtaagg cgaatacggt gtgactgaac
     1141 ttgacgaggg agaggtagtt tcgaaaaacg gcctgcatac cccgcaaagg taataaaccg
     1201 gcggggctat gaaccgttgt gtttaacgta ccactgccag ccggatggtg cgcaggatgt
     1261 cgcttctgat ctcgacctgc ggcttgacgg tg
//
LOCUS       DAOTON010000854         1291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_84068_length_1291_cov_2.521036, whole genome shotgun sequence.
ACCESSION   DAOTON010000854 DAOTON010000000
VERSION     DAOTON010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84068_length_1291_cov_2.521036"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="P5292_13375"
     CDS             complement(<1..571)
                     /locus_tag="P5292_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HRS40165.1"
                     /translation="MNTNEIKARFGIIGGSPLLEHAINVARQVAPTEISVLIFGESGV
                     GKEVFPQIIHQLSPRKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGAHEARKGYFEV
                     ANGGTIFLDEVGELPLNTQVRLLRVLETGEFIKVGSSKVQKTNVRIVGATNVDMQQAI
                     ANGKFREDLYYRLNTVPIRIPPLRDRKEDI"
     gene            complement(587..>1291)
                     /locus_tag="P5292_13380"
     CDS             complement(587..>1291)
                     /locus_tag="P5292_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRS40166.1"
                     /translation="QQQFVTFADLMERVAQVSPKLRIRFSTSNPRDITEAVLEVMAKY
                     PNICKYIHLPVQSGNSEVLEKMNRGYTREDYMQRIEAIRRIVPGCGVSTDIISGFCGE
                     TEEQHRDTLSLMQWSGYDYAFMFKYSERPGTLAARKYPDDIADEVKTRRLTEIIDLQN
                     KLSLESNRRDIGRTFEVLVESLSKRSDAHLSGRTTQNKVVVFPKQHYQPGDYVFVKVN
                     DCTSATLLGEATGRAD"
BASE COUNT          228 a          362 c          395 g          306 t
ORIGIN      
        1 agatgtcttc cttccggtca cgcagcggcg ggatccggat cggaacggtg ttgaggcggt
       61 agtagaggtc ttcgcggaac ttgccgttgg caatggcctg ctgcatgtca acgttggttg
      121 cgccgacaat gcgcacgttc gtcttctgca ctttcgagga accgactttg atgaactctc
      181 cggtctccaa tacgcgcaac aggcggacct gggtgttgag cggaagctcg cccacttcgt
      241 cgaggaagat cgtgccgccg ttcgctactt cgaagtaacc tttccgtgct tcatgcgcgc
      301 cggtgaacga gcctttctcg tgaccgaata gttccgagtc gatcgtgcct tcgggaattg
      361 cgccgcagtt cacggcgatg tacgggccgt gcttgcgcgg actgagctgg tggatgatct
      421 gcgggaacac ttccttaccc acgccgcttt ccccgaagat caggacgctg atctcggtcg
      481 gcgctacctg tcgggctaca ttgatggcat gctcgagcag gggagagccg ccgatgatgc
      541 cgaaacgcgc tttgatctcg ttcgtgttca tgctggtttc agtcgtttag tccgcgcgac
      601 ctgtcgcttc gcccagcagg gtggcggagg tgcaatcgtt cactttaacg aagacgtaat
      661 cgccgggctg atagtgttgc ttgggaaaca ccaccacttt attctgggtg gtgcgaccgc
      721 tgaggtgcgc gtccgaacgc ttgctcagcg actccaccag tacctcgaag gtgcgaccga
      781 tgtcgcgccg gttgctctcg agcgaaagtt tgttctgcag gtcgatgatc tcggttagcc
      841 ggcgggtttt gacctcgtca gcgatatcgt cgggatactt ccgcgccgcc agggtgccgg
      901 ggcgttcgga gtatttaaac atgaaggcgt aatcgtagcc ggaccattgc atcaacgaca
      961 aggtgtcgcg atgctgttct tcggtctcgc cgcagaatcc gctgatgata tcggtggaaa
     1021 caccgcagcc cggtacgatc cggcggatcg cttcgatgcg ctgcatgtag tcttcccgcg
     1081 tgtatccgcg attcatcttt tcgagcactt cgctgttgcc cgactgcacc ggaaggtgga
     1141 tgtacttaca gatgttggga tacttcgcca tcacctccag caccgcttcg gtgatgtcgc
     1201 gcggattgct ggtggaaaaa cggatccgca gcttcggact gacctgcgcg acgcgctcca
     1261 tcagatcggc gaaggtgacg aactgttgct g
//
LOCUS       DAOTON010000855         1290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_84174_length_1290_cov_2.498785, whole genome shotgun sequence.
ACCESSION   DAOTON010000855 DAOTON010000000
VERSION     DAOTON010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84174_length_1290_cov_2.498785"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1290
                     /locus_tag="P5292_13385"
     CDS             <1..>1290
                     /locus_tag="P5292_13385"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRS40167.1"
                     /translation="RGTQADGHGFISSVVEQGAAAIVCETLPEQRVAGVTYVRVQDSA
                     LALGQIAANFFGNPSEKLKLVGVTGTNGKTTTVTLLFQLFRRLGYGVGLLSTVRNQIN
                     DQVIPSTHTTPDPIQLNALLSAMVDEGCTHAFMEVSSHAVAQKRIAGLRFAGGVFTNI
                     THDHLDYHKTFEEYLKAKKGFFDQLSSDAFALVNADDRNGAVMLQNTRARKYTYALRT
                     MADYKARLMENQFSGLLLNIDGQEVLCRLVGSFNAYNLTATYAAAVQLGESKMEVLTA
                     LSLLAPVEGRFDYVASPANVIGVIDYAHTPDALQNVLSTIHDVMAGQGRVITIVGCGG
                     DRDAAKRPVMARIACELSDQVILTSDNPRSEDPAEIIRQMERGIPPTDSRKSLSIADR
                     KEAIKTAVTLARSGDIILVAGKGHEKYQEIKGVRHPF"
BASE COUNT          291 a          358 c          378 g          263 t
ORIGIN      
        1 gcgcggaacg caggcagacg ggcatggctt tatctccagt gtcgtggaac agggtgcagc
       61 cgccatcgtt tgtgaaacac ttccggagca gcgtgtcgcc ggcgtaacct atgtgcgggt
      121 gcaggacagt gccctggcct tgggacagat cgcggccaat ttcttcggca atccttctga
      181 aaaactgaag ctggtcggcg taaccggaac gaacggcaag accaccaccg tcaccttgtt
      241 gtttcaactg tttcgccgtc tgggctatgg tgtcggcttg ttgtcgactg tccgcaacca
      301 gatcaacgac caggttattc cgtcaaccca tacgactccc gacccgattc agttgaatgc
      361 gctgctatcc gcgatggtcg acgagggttg tacgcacgcg ttcatggaag tgagttcgca
      421 tgcggtcgcg caaaaacgca tcgccgggct gcgtttcgcg ggcggtgtct tcaccaatat
      481 cacacacgac catctcgatt atcataagac gttcgaggag tacctcaaag ccaagaaagg
      541 cttcttcgac cagctttcat ccgatgcatt cgcgctcgtc aatgccgatg accgcaatgg
      601 tgcggtgatg ttgcagaata cgcgcgcacg caaatacact tatgcgctgc ggacgatggc
      661 cgactataaa gcgcggttga tggagaatca gttttccgga ctgctcctga acatcgacgg
      721 acaggaggtg ctgtgtcgcc tggtcggcag tttcaacgcc tacaacctga ccgctaccta
      781 cgctgcagcc gtacaactcg gagaaagcaa aatggaagtc ctgaccgccc tgagtctgct
      841 ggcgccggta gaaggacgat tcgattatgt cgcgtcaccg gccaatgtga tcggtgtgat
      901 cgactatgca cacacgccgg atgcgctgca gaatgtgctc agcaccattc acgatgtcat
      961 ggccggacaa gggcgcgtca ttacgatcgt cgggtgtggc ggtgatcgcg acgcagcgaa
     1021 gcgtccggtg atggcgagga tcgcctgtga gttgagcgac caggtcatcc tgaccagcga
     1081 taatccgagg tcggaggatc cggccgagat catccggcaa atggaacgcg gcattccacc
     1141 gaccgacagc cgcaaatcgc tcagcattgc cgatcgcaaa gaagctatta aaacagcggt
     1201 cacactcgcg cggtctggag atatcatcct ggtcgcgggc aagggtcacg aaaagtatca
     1261 ggagatcaag ggagtgcgtc atccgttcga
//
LOCUS       DAOTON010000856         1289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_84286_length_1289_cov_2.330632, whole genome shotgun sequence.
ACCESSION   DAOTON010000856 DAOTON010000000
VERSION     DAOTON010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84286_length_1289_cov_2.330632"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="P5292_13390"
     CDS             <1..301
                     /locus_tag="P5292_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRS40168.1"
                     /translation="FTVAFYSAAQAEKMGLVNNVYTDQAALYEAARQMAAQITENAPL
                     AIECTKEVLNFSRYTSVSEGMALAIQKNMFLLTTDDIKEAFIAFMEKRQPNFKGK"
     gene            complement(382..>1289)
                     /locus_tag="P5292_13395"
     CDS             complement(382..>1289)
                     /locus_tag="P5292_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRS40169.1"
                     /translation="KLAKDVCVFIRSLGIQLSEGYGLTETSPVINFNEPEFNTSFDIS
                     KANWFQNKMLDLTVDLMVTKQAQGLSPYTNPIRALGLALAYNTVAHKLQIKPGTVGKP
                     ALWTEEKIAEDGEILVKGPQVFKGYWNLPEATKEAFTEDGWFKTGDIGEFDSDGFLRI
                     TDRKKELFVTSGGKNVAPHPIELAITGKPYIDQACLIGDAKKYLTALIVPDFPELHRY
                     AKHNGIPACSDKDLIANDQIKALIQKQVDEVNETLPRYEQIKYYKVLDKPFSVETGEL
                     TPTMKLKRRIVLENYKNEIESMYQS"
BASE COUNT          306 a          294 c          310 g          379 t
ORIGIN      
        1 ctttaccgtc gctttttatt ctgcagcgca ggctgaaaaa atgggactgg tcaacaatgt
       61 ctataccgat caagccgcgc tttatgaggc ggcccgccag atggcggcgc agataaccga
      121 gaacgcccct cttgcgattg aatgtaccaa ggaagtgctt aattttagtc gctacaccag
      181 tgtgagtgaa ggcatggcac tggcgataca aaaaaatatg tttctgctga ccaccgatga
      241 tatcaaagaa gcctttatcg ctttcatgga aaaacgacag ccgaatttta aagggaagta
      301 agaagtcagt tgcttcattg cgcatgaaaa aagagggatg gcccatggac gatccctctt
      361 tttttcgatg aaaattattt tctaactctg gtacatgctt tcgatttcgt ttttgtagtt
      421 ttcgagaaca attcttcgct tcagcttcat cgttggggtt agttctcccg tctcgacgct
      481 gaaaggtttg tccagtactt tgtagtactt gatttgttcg tagcggggca gtgtttcgtt
      541 gacttcatcc acctgcttct ggatcaacgc cttgatctgg tcattagcaa taagatcctt
      601 gtcggaacat gccggaatcc cgttgtgctt tgcataacga tgcagttccg gaaagtcagg
      661 cacgatcaga gcggttaaat attttttcgc gtcgccgatg aggcaggcct gatcaatgta
      721 tggtttgcct gtaatggcca gttcaatggg atgcggggct acatttttgc cgccggaagt
      781 tacaaacaat tctttcttgc gatcggtgat gcgaaggaat ccatcactgt caaattcacc
      841 gatatcccct gtcttgaacc atccgtcttc cgtgaaagct tcttttgttg cttcaggtaa
      901 attccagtat cccttaaata cctgcggacc cttcaccaga atttcaccat cttcagcgat
      961 tttttcctcg gtccagagag ccggtttgcc taccgttccg ggcttgatct gtagtttatg
     1021 agccacggtg ttataggcca gagcgagacc caaggcgcga atcgggttgg tataagggga
     1081 tagaccctgt gcctgtttgg tgaccatcag atccacggtc aggtcaagca tcttgttttg
     1141 aaaccagttg gctttgctga tatcaaaact ggtgttgaat tccggttcgt tgaaattgat
     1201 gaccggtgat gtttccgtca ggccgtaacc ttcacttaat tgaatgccca gtgaacggat
     1261 aaacacgcaa acgtctttgg caagtttgc
//
LOCUS       DAOTON010000857         1288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_84366_length_1288_cov_4.402271, whole genome shotgun sequence.
ACCESSION   DAOTON010000857 DAOTON010000000
VERSION     DAOTON010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84366_length_1288_cov_4.402271"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="P5292_13400"
     CDS             <1..1191
                     /locus_tag="P5292_13400"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRS40170.1"
                     /translation="ALLLFFIGSLFLQSGINAQDNAAPSFNFKNGLGIASPDSAFTMN
                     IRFRIQNRAAFTVAQEDEDTWDVSAVDARVRRMRLRFEGQVYDPRLTYLIQLSFSRGD
                     MDWDNSGVPNVLRDAMVFYKVSNKLNIALGQGKLPGNRQRVVSSGDQQFADRSIVNAA
                     LNIDRDFGFQFNYTEKTGDVYYILRGAVSSGEGRNSAGSDKGLCYTGRLELLPFGLFD
                     RRGDYFEGDLAREKKPKLSLAGGLSFNDRSVRTGGQLGRDLYSPVDIQTAIFDGLFKY
                     QGIALYGEYMQRETPEFSPVTENSSGAKRYVFAGEGMLAQASYLFKSNYEIAARYALT
                     HPAASIQSLEPERTNYTLGVTKYLKAHRVKLQSDVTYEETLNLSNDTEGKNWQFRFQV
                     ELGI"
BASE COUNT          302 a          358 c          335 g          293 t
ORIGIN      
        1 gcattactac tattctttat tggcagccta ttcctgcaga gcggcatcaa cgcacaggac
       61 aacgccgctc caagcttcaa cttcaagaac ggcctgggca ttgccagtcc ggacagcgcg
      121 ttcaccatga acatccgctt ccggatccag aaccgggctg cattcaccgt cgcacaagag
      181 gacgaggaca cctgggatgt atctgccgta gatgcccgcg tccgacgcat gcgcctccgc
      241 tttgaaggac aggtgtacga tccccggctc acctacctga tccaactgag cttcagtagg
      301 ggcgacatgg actgggacaa ttccggtgtg ccgaatgtgt tgcgtgacgc catggtgttt
      361 tacaaagtca gcaacaaact caacatcgcc ctggggcagg gaaagctacc cggtaatcgt
      421 cagcgtgtcg tttcttccgg agaccagcag tttgccgacc gttcgattgt taatgccgcg
      481 ttgaacattg accgggattt cggttttcag ttcaactata ccgagaaaac cggagacgtt
      541 tactacatcc tgcgtggcgc ggtgagttcc ggcgaaggac gcaactccgc aggctccgac
      601 aaaggattgt gttatacggg ccgactggaa ttactcccct tcggcctgtt cgatcgaaga
      661 ggagattatt tcgaaggcga cctggcccgg gagaaaaagc cgaaactgtc gctggccggt
      721 ggcttgtctt tcaacgaccg ctccgttcgt accggcggac aactcgggcg agatctttat
      781 agtcctgttg atattcaaac agccattttc gacggactgt tcaaatacca gggcatcgcg
      841 ctttacggtg aatacatgca acgcgaaact ccggaatttt ctcctgtgac ggaaaacagc
      901 agtggcgcca agcgctatgt atttgccggc gaaggcatgc ttgcccaggc gtcttacctt
      961 ttcaaaagca actatgaaat cgccgcccgg tatgccctca cccatccggc tgcttcgatt
     1021 caatcccttg aaccggaacg caccaattat acgctgggcg tgaccaagta tctcaaagcg
     1081 caccgcgtca agctccagtc cgatgtaact tatgaggaga cgcttaactt gtccaacgat
     1141 accgaaggaa agaactggca gttccgtttc caggtggaac ttggcatctg atcagtttgg
     1201 tattaaaaag acgagcgcgc agaagtcatc cgcgcgctcg tctttttaag ttagcagctg
     1261 gttattcgac taccagtcgg ctcatggc
//
LOCUS       DAOTON010000858         1288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_84423_length_1288_cov_1.904298, whole genome shotgun sequence.
ACCESSION   DAOTON010000858 DAOTON010000000
VERSION     DAOTON010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84423_length_1288_cov_1.904298"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1288
                     /locus_tag="P5292_13405"
     CDS             <1..>1288
                     /locus_tag="P5292_13405"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS40171.1"
                     /translation="TGTYTVMLIVQTATGCIDTVRQSLLLSNNRPTAAFTTTNVCFGE
                     QANFDDGSASNITVWDWDFGDGNGSNVPSPAHTYNASGNYVVTLSVTNADGCTSTTTR
                     PITIYTLPTANAGPNDTICRGSVATLTASGGVSYAWYPGGGTTATFTVSPTSTQDFYV
                     VVTDARGCTDRDSARVILRRSPNVSAGNTRRICLGDTTTLSAQANGSQPLTYLWTPGN
                     LATQQIQVHPNTTTNYYLTVRDSYGCAGYDTVQVIVNPLPTAATNPNQLICPGDSALL
                     IASGGLSYSWSGGGNNDSVYVTPATTTAYTVTVADANGCEDTEDILVTVSAPATANLG
                     PDRTICAGSSVSLAVGNGVAYLWNPGGQTTSSVSVSPLVTTDYTIRVTNFAGCRAWDT
                     IRVNVNDPPVIAAQVLGDVSCFGGADGQAMVAVNAGT"
BASE COUNT          260 a          407 c          366 g          255 t
ORIGIN      
        1 accgggacct acacggtgat gctcatcgtg caaacggcca ccggttgtat cgataccgtg
       61 cggcagtcgc tgctgttgtc aaacaaccgc ccgacggctg ctttcaccac taccaatgtt
      121 tgttttggcg aacaggcgaa tttcgacgac ggcagcgcgt ccaacatcac ggtgtgggat
      181 tgggatttcg gcgatggcaa cggttccaat gtgccatcac cggcgcatac gtacaacgca
      241 tccggcaatt atgtcgtgac gttatccgtc accaatgcgg acggatgtac ctcgacgaca
      301 acgcggccga tcacgatcta cacactgccc acggcaaatg ccggccccaa tgacaccatt
      361 tgccggggct ccgtggctac gctcaccgcg agcggcgggg tctcgtatgc ctggtatccg
      421 ggtggaggaa cgacggcgac cttcacggtt tcaccgacca gtacccagga cttttatgtg
      481 gtcgtaaccg acgcgcgcgg atgtaccgac cgcgacagcg cccgtgtgat cctgcgcaga
      541 agtccgaatg tcagtgcagg taacacacga cggatttgtc tgggcgatac caccaccctg
      601 agcgcacagg cgaacggctc gcaaccgctt acctatctct ggactccggg caacctggct
      661 acgcagcaga tacaggtaca tccgaacacc acgaccaact actacctcac cgtgcgggac
      721 agctacggct gcgcgggata cgatacggtg caggtgatcg tcaatccgct gccgaccgcc
      781 gctaccaatc cgaaccaatt gatctgcccg ggcgattccg ccttgttgat cgcctctggc
      841 ggcctcagtt actcctggag cggaggcggg aacaacgatt cggtttacgt aacgccggct
      901 accaccaccg cctataccgt caccgtggcg gatgccaacg gctgtgaaga caccgaagat
      961 attctggtca ccgtctccgc gccggctacc gcgaaccttg gccctgaccg gacgatctgt
     1021 gccggaagct ccgtgtcgct ggctgtcggc aatggcgtcg cctacctgtg gaatcctggc
     1081 ggtcaaacga cttcttccgt gagtgtaagt ccgctggtta ccaccgatta cacgatcagg
     1141 gtcaccaact tcgccggctg tcgggcctgg gataccattc gggtcaatgt gaacgatcct
     1201 ccggtcatcg ctgcgcaagt gctcggcgat gtttcctgtt tcggtggcgc cgacggacaa
     1261 gccatggttg cagtgaatgc cggaactc
//
LOCUS       DAOTON010000859         1283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_84850_length_1283_cov_4.433225, whole genome shotgun sequence.
ACCESSION   DAOTON010000859 DAOTON010000000
VERSION     DAOTON010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84850_length_1283_cov_4.433225"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="P5292_13410"
     CDS             <1..244
                     /locus_tag="P5292_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40172.1"
                     /translation="VYPNPASSRVVHLSYGDDQMQFYTITVRDITGREIPATVSRTGN
                     GDIQLEFSEIYMKPGSLYFIVATDGIETVQQKLLIQ"
     gene            365..>1283
                     /locus_tag="P5292_13415"
     CDS             365..>1283
                     /locus_tag="P5292_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRS40173.1"
                     /translation="MKLVTYRLGNRERIGLWHDGSIYNLPDAAKSLGFTALPDDMLEF
                     IRAGEASWEVARKINAKIRSGNSDLSPEAVGENALMAPVPRPTSCRDGYAFRQHVAAA
                     RRNRKVPMIPEFDQYPIFYFTNHNAIQGPGEIRCMPDHFQKLDFELESAIVIGKKGRN
                     IRASEADQYIFGYLIMNDMSARTLQMEEMVLNLGPAKGKDFSTVIGPWLVTPDELESH
                     RVSPKPGHVGNSYDLTMKCWVNDKLVSSGNMKDMDWTFAEIVERCAYGVDILPGDVIG
                     SGTVGTGCFLELNGTGLLQDPNYPVQWLQD"
BASE COUNT          299 a          337 c          358 g          289 t
ORIGIN      
        1 cgtgtacccg aacccggcca gcagtcgggt ggtccacctg agctacggcg acgaccagat
       61 gcagttctac accattacgg tgcgcgatat cacgggtcgg gagatccctg ccaccgtgtc
      121 acgcaccggt aacggggata tacaactgga attctcagaa atctatatga aacccggctc
      181 gctttacttt atcgtggcaa ccgacggaat cgaaacggtt cagcagaaac tgctgatcca
      241 gtaagacacc tttcatggtt tggtttggat gattggttag acaaccggag ggcccctatg
      301 gggcctttcg gttttttcgg gggtctcctc cccgtttcgt atttttgtga aaagccaatt
      361 cctcatgaaa cttgtcacct atcgccttgg caaccgggaa cgtatcggcc tttggcatga
      421 tggatccatc tataacctgc cggatgctgc caaatcgctc ggatttacag ccctcccgga
      481 cgatatgctc gaattcatcc gggccggtga ggcatcctgg gaagtcgcac ggaagatcaa
      541 tgcgaagatc cgatccggca acagcgatct tagcccggag gcggttggtg aaaacgccct
      601 gatggcacct gttccccgtc ccacttcatg tcgtgacgga tatgcgttcc gccaacatgt
      661 agcagcggcc agaaggaatc gaaaagtccc catgatcccg gagttcgatc agtatccgat
      721 cttctatttc acgaatcaca acgccatcca ggggccagga gaaatccgtt gtatgcctga
      781 ccatttccaa aagctggatt tcgaactgga gtcggcgatc gtcattggta agaagggacg
      841 caacatccgg gcatcggaag ccgatcaata tattttcggc tatctgatca tgaacgatat
      901 gagcgcgcgg acacttcaaa tggaagaaat ggtattgaac ctcggaccgg ccaagggcaa
      961 ggacttctcc accgtcatcg gcccctggct ggtgacgccc gacgaattgg agagccaccg
     1021 cgtcagcccc aagccgggtc atgtaggcaa cagctatgat ctgactatga aatgctgggt
     1081 gaacgataag ttggtcagca gtgggaacat gaaagacatg gactggacgt ttgccgagat
     1141 cgtggagcgc tgtgcttacg gcgtggatat acttcctggc gacgtgatcg gctccggcac
     1201 ggttggtacg ggttgttttc tggaattgaa cggcaccgga ttgctgcagg atccgaacta
     1261 tccggttcag tggttgcagg acg
//
LOCUS       DAOTON010000860         1278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_85371_length_1278_cov_4.085854, whole genome shotgun sequence.
ACCESSION   DAOTON010000860 DAOTON010000000
VERSION     DAOTON010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85371_length_1278_cov_4.085854"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..765)
                     /locus_tag="P5292_13420"
     CDS             complement(178..765)
                     /locus_tag="P5292_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40174.1"
                     /translation="MTARSRYRIFLLLLLLGPLRVSAQTVDISIGGSSNVDFTFNTIQ
                     KLTTGIVIPNAFTVNVEAIGTNWDLYMGAVTAVAGTWDNAQYYATSGNGAPPVGLLQA
                     RVHNLSGTPQISGYVPLQDIAVSTLDIIGNHLSAPDPSVNCGDASPIGTNTPGSYLTD
                     PQCYQFRVDLKVVPGMNYRPGLYTMQIEFIIAPDL"
     gene            complement(888..>1278)
                     /locus_tag="P5292_13425"
     CDS             complement(888..>1278)
                     /locus_tag="P5292_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40175.1"
                     /translation="GTLPSEILEMQAIQPNAVAAALTFNSFIGLSSNAGANTASLTPT
                     VDTQFLAGQFGQGVANSYAPATSTASPLTNKFLVHYRIKPGVPANFPNLTTQYPTVAA
                     ATFTATAPYAQPGYYDLEVVYTLTEDL"
BASE COUNT          297 a          320 c          383 g          278 t
ORIGIN      
        1 tataacgttt taacgtcgaa agttgaaggt tgaaggttga aggttgaaag taaagaccgc
       61 cttattcagt tgttcctggt ctacaattac taattactag ttactaatta ctagttacca
      121 ggccccccaa cttccccgtc acgctaaaag caccaaagca cggccgcctt ttcgatccta
      181 aagatccggc gcgatgatga attcgatctg catcgtgtac aaacccggac ggtaattcat
      241 gccgggaacg actttcagat ctacacggaa ctggtagcat tgcgggtcgg tcaggtaact
      301 tcccggcgta ttcgttccta tcggtgaagc atcaccgcaa ttcacggaag ggtcgggagc
      361 ggaaaggtga ttaccgatga tatcgagtgt cgacactgca atgtcctgca agggcacgta
      421 gccgctgatc tggggcgtac ctgaaaggtt gtgcacgcgg gcctgcaaca agccaaccgg
      481 aggtgcgccg tttccgctag tcgcgtagta ctgcgcgttg tcccaggtac cggccacagc
      541 cgtgaccgcg cccatgtata aatcccagtt ggtgccgatc gcttccacgt tcaccgtgaa
      601 ggcattcggg attacgatgc cggtggtcag cttttggatc gtattgaacg taaaatcaac
      661 attcgaactt ccaccgatcg agatgtccac cgtctgcgcg gaaacccgca gcgggccgag
      721 tagcaacagc agcaggaaaa ttcggtatcg gcttcgggcg gtcataagaa atgctcctgg
      781 tatgtactgc ttcgaccgga gaaggttgca ggggtaaaaa agaaaaagag gagcgggggg
      841 ccgtctcctc tttttccagg atgtaaatcg ggggtgtcgg gaatggctta gaggtcttcc
      901 gtcaacgtgt acaccacttc gagatcgtag taacccggct gggcatacgg tgcggtagcg
      961 gtgaaggtgg cggcagcaac cgtcggatac tgcgtcgtca ggttcgggaa gttggcagga
     1021 acacccggct tgatgcggta gtgaaccagg aacttgttcg tgagcggcga agccgtcgag
     1081 gtggcgggag catagctgtt ggctacaccc tgaccgaact gaccagcgag gaactgcgtg
     1141 tcaacggtcg gcgtcagcga agcggtgttc gcgccggcat tcgacgatag tccgatgaag
     1201 ctgttgaagg taagcgcagc ggcaaccgcg ttcggctgta tggcctgcat ttcgagaatt
     1261 tcagaaggca gcgtgccc
//
LOCUS       DAOTON010000861         1276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_85599_length_1276_cov_2.651925, whole genome shotgun sequence.
ACCESSION   DAOTON010000861 DAOTON010000000
VERSION     DAOTON010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85599_length_1276_cov_2.651925"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1276
                     /locus_tag="P5292_13430"
     CDS             <1..>1276
                     /locus_tag="P5292_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40176.1"
                     /translation="FPGPARRNAFTWSINGKIYLGCGNQGANCYRDLWQYDPATDSWL
                     QRTDFPGNNWNQYAAFTYNGKGYVVFGYASCGSSSASSQFWEYDPQTDSWNQLADVPF
                     VSIVSRGAVLGNKVYFLDTSGAWTYSYDFSSQTWSNSWPNAAGSGTGAPVFSTTIQGV
                     VHCFTENFVKYAHLPSTSWLRGTYLHNSLFHSETAFMAFADSVKIIGGGTGLSAFTQD
                     VWTYYPGCASTITAAITAPDTSFVGARNPLLNSSSIAPTTSQFLYHWRVDTADWGWFS
                     GDTAVVAFRYGTVPVSMVATNGYCYDSIATGVHIAQGMWSGKTTLPLEAKGRNFAVGA
                     GNAGVAYFGLGNDEDNNLLRDFWRYDCNSQRLERLPDFPFSPVHYGKFIFAAGHYYLL
                     GGDTNVAPWKTNLFYEFLPATGGWNRLPDFPGP"
BASE COUNT          278 a          332 c          332 g          334 t
ORIGIN      
        1 atttccgggt cctgcacgtc ggaacgcatt tacctggtcg atcaacggga aaatctattt
       61 aggctgtggc aaccagggcg ccaattgtta ccgggacctt tggcaatatg atccggcgac
      121 cgatagctgg ctgcagcgaa ctgattttcc cggcaacaac tggaaccaat atgcggcatt
      181 cacttataac ggaaaaggtt acgtcgtttt tggttatgcc tcctgcggat catcttcagc
      241 ctcatcacaa ttttgggaat acgaccctca aaccgacagt tggaatcaat tggccgatgt
      301 accctttgtc agcatagtta gccgcggagc cgtcctgggg aacaaagttt attttctgga
      361 cacatccggc gcgtggacct atagctatga tttctcttcc cagacttggt ccaacagctg
      421 gcccaatgcc gcgggaagcg gtaccggtgc cccggtgttt tcaacgacga ttcaaggagt
      481 cgtccactgt tttacggaga actttgtcaa gtatgcccac cttcccagca catcgtggct
      541 gcgtggtacc taccttcata atagtctgtt tcattcggag acggccttta tggctttcgc
      601 cgattcagta aaaataattg gcggtggtac cggcttgtcg gctttcacac aggatgtatg
      661 gacgtactat cccggatgtg ccagcaccat cacggccgct attacagctc ccgatacgag
      721 ttttgttggc gcaagaaatc ccctcctcaa ttccagttcg atcgctccaa cgaccagcca
      781 gtttttatat cactggcgtg tcgatacggc cgactggggt tggttttccg gcgatacggc
      841 tgtcgtagcc tttcgttatg gtacagtgcc ggtgtccatg gtggcaacga atggctattg
      901 ttacgactcc attgcaacgg gagttcacat cgcgcaaggg atgtggtccg gaaagaccac
      961 tttgccgctg gaagccaaag gacgcaactt cgcagtagga gccggtaacg ccggagtcgc
     1021 ctactttggt ttgggtaatg atgaggacaa taatctgtta cgcgactttt ggcggtacga
     1081 ttgtaacagt cagcggttgg agcgattacc cgattttcct tttagccccg tgcattacgg
     1141 taaattcatc tttgcggccg gacattatta cttactcgga ggcgatacga atgttgctcc
     1201 gtggaagacg aatctctttt acgaattctt gcccgcgacc ggcggctgga accgactgcc
     1261 ggatttcccg ggacca
//
LOCUS       DAOTON010000862         1271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_86103_length_1271_cov_2.886513, whole genome shotgun sequence.
ACCESSION   DAOTON010000862 DAOTON010000000
VERSION     DAOTON010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86103_length_1271_cov_2.886513"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="P5292_13435"
     CDS             complement(<1..515)
                     /locus_tag="P5292_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRS40177.1"
                     /translation="MSKHRVPKGLLEIYGARVHNLKNIDVTLPREQLVVITGLSGSGK
                     SSLAFDTIYAEGQRRYLESFSAYARQFLGNMERPDVDKISGLSPVISIEQKTTNLNPR
                     STVGTITEIYDFLRLLFARASEAFSYVTGEKMIRLSDEQIVELIQKKFDGRKVLLLSP
                     LVKARKGHYRE"
     gene            complement(585..>1271)
                     /locus_tag="P5292_13440"
     CDS             complement(585..>1271)
                     /locus_tag="P5292_13440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40178.1"
                     /translation="NSQPTVTLRSTSANGYAWSSGQTTRNITVNSQGSYRVTAIAANG
                     CRATSAPVIVSANGCTPPAVPVITVSGSTVLIPGQTVTLTSSAAGGWLWSTGATTQSI
                     VVSTAGTYTVRAYNAGSCFSTSNPVTVLLVSARFSADLSQPSTQSVPVLYPNPVKSTA
                     HLEYTAVTEGTARIRVFDLTGRLQLDQEFPSEPGLNRVEINTDPLPAGSYVLLLSGEQ
                     ERSVRMVVQD"
BASE COUNT          283 a          341 c          369 g          278 t
ORIGIN      
        1 aattcccggt aatgtccttt acgtgctttg accaggggtg aaagcaggag cactttgcgc
       61 ccgtcaaact tcttttgaat caattcgacg atctgctcat cactcaggcg gatcatcttc
      121 tcgcccgtca catagctgaa tgcttccgaa gcacgggcga acagcaggcg aagaaagtcg
      181 tagatctcgg taatggtgcc aacggtagaa cgtgggttca ggttcgtggt cttctgctca
      241 atcgagatga ccgggctcag tccgctgatc ttgtcgacat ccggccgttc catgttgccc
      301 aggaactgcc gggcataggc ggaaaaactc tccaggtagc gacgctgacc ttccgcatag
      361 atcgtgtcga aggcaagcga tgacttcccg cttccgctca gtccggtgat gaccaccaac
      421 tgttcacgcg gcagggtgac atcgatgttt ttcaggttgt gaacccgggc gccataaatt
      481 tccagcaagc ccttgggtac gcggtgcttc gacatataca gactacgccc tcccgctacg
      541 ggagaaaacg gcaatctgcg aaattacgca aaaagggcgt tcaattaatc ctgaacgacc
      601 attcggaccg atctttcttg ttcgccactg agtaacagca cataagaacc agcagggagc
      661 ggatccgtgt tgatctccac gcgattaagg ccgggctccg acgggaattc ctgatccagt
      721 tggaggcgtc ccgtcagatc gaatacgcgt atacgcgcag ttccttctgt aactgcggtg
      781 tactccaggt gggcggtgga tttcaccgga ttcgggtaaa gcactggcac cgactgtgtc
      841 gatggctgtg acaggtcagc actgaaacgg gcactgacca ggagcactgt cacaggattg
      901 gaagtggaaa agcaactccc ggcgttgtat gcgcgaacgg tataggttcc ggcagtggat
      961 actacgatcg actgcgtcgt tgctccggtc gaccacagcc atccgcctgc ggcgctgctg
     1021 gtcaaagtca cggtttgtcc gggaatcagt acggtggaac cactgacggt aatgaccgga
     1081 acagcgggcg gggtacaacc attcgcagaa acgatcaccg gggcggaagt ggccctgcaa
     1141 ccattggctg caatggcagt gacccggtaa ctgccttgtg aattgacggt gatgttacgc
     1201 gtggtctgcc cgctggacca tgcatacccg tttgccgagg tggagcgtag cgttacggtg
     1261 ggctgactat t
//
LOCUS       DAOTON010000863         1269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_86346_length_1269_cov_2.420099, whole genome shotgun sequence.
ACCESSION   DAOTON010000863 DAOTON010000000
VERSION     DAOTON010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86346_length_1269_cov_2.420099"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1269
                     /locus_tag="P5292_13445"
     CDS             <1..>1269
                     /locus_tag="P5292_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40179.1"
                     /translation="GNPSSNNLKLVIKSPSITWSGSGQITTSNPGTGSAYGNISFARA
                     GTSTFNRAGTHLVQQVYMTVESGNTLDLAIGNIQLSSHPTMAGLPLFLMVNNNGVLNM
                     RTNKLFSNALEMYSGISVLGRVRTQNVNGIYDATTNASFSTTVDDSLDYYLSSGSTVE
                     YNGVDNQIVSGVGLGRARSMQHRYGNLEINFGGTANTEFVYPTNAPNDSAVWVRGNLL
                     LTNGELNLDNDHVPSNSGGRMVYVETSAATGIQRTNGYIRSETEDGSGRLKWNIAAST
                     GAHVVPFGYDASNYIPFTYNMASGSTGSLYAATYRTNAANLPYPPTVTHVRNNSNVDN
                     SANTVDRFWYLQVSGTTTNAALTFVCTPSELGSIASPRAQRWVAPMVSWTNPPPGTQS
                     NPASNSALATGVGVYNNWWTLAGASNLLPI"
BASE COUNT          310 a          348 c          324 g          287 t
ORIGIN      
        1 acgggaatcc gtccagcaat aaccttaaac ttgtcatcaa aagcccgagc atcacctggt
       61 cgggaagtgg acagattacc acatccaatc ccggcactgg aagcgcatat ggcaatatct
      121 catttgcccg agcaggaact tccaccttta atcgtgcagg cacgcacctg gttcagcaag
      181 tgtatatgac agtggagtcc ggtaacacgt tggatcttgc cattggcaac attcaattgt
      241 ccagccaccc gactatggcc ggcttgccgc ttttcctgat ggtgaacaac aacggggtac
      301 tgaacatgcg taccaacaag ttgttctcca acgcgctcga aatgtattcc ggcatttccg
      361 tactgggtcg tgttcgcacc cagaacgtga acggtatcta tgatgctaca acgaatgcca
      421 gcttctctac aacagtggac gatagtctgg actactacct ttcgtctggc tcgacggttg
      481 aatataacgg tgtcgataat cagattgtat ccggagtcgg gcttggccgc gcccgcagta
      541 tgcagcatcg ttatggcaat ctggaaatca atttcggcgg taccgccaat acggagttcg
      601 tatatccaac caacgctcca aatgattccg cggtatgggt acgcggaaac ttgctgttga
      661 ccaatggcga attgaacctg gacaacgatc acgtcccttc gaatagcggc ggtcgaatgg
      721 tatatgtcga gaccagtgca gcaacgggaa tacaacgtac caacggctat atccgttcgg
      781 aaaccgaaga tggatcgggg cgtttgaagt ggaatatcgc tgcaagtacc ggcgcgcatg
      841 tggttccttt cggttatgac gcgagcaact atattccgtt cacgtataat atggcttcgg
      901 gaagtacggg aagcctctac gcggcaacct atcggacgaa cgcagccaac ctaccctatc
      961 cgccgacggt cacacatgtt cggaataaca gtaatgtgga taacagtgcc aacacggttg
     1021 accggttctg gtatctccag gtcagtggta ccactacgaa cgcagccttg acgtttgtct
     1081 gcacaccttc cgagctcgga tccatcgcca gcccacgggc tcagcgctgg gttgcaccta
     1141 tggtttcctg gaccaatccg cctcccggca cccagagcaa tccggcatcc aattccgcgc
     1201 ttgcaacagg cgtaggagtt tacaataact ggtggacgtt ggcgggagcc agtaacctcc
     1261 tgccgatcg
//
LOCUS       DAOTON010000864         1264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_86842_length_1264_cov_3.460711, whole genome shotgun sequence.
ACCESSION   DAOTON010000864 DAOTON010000000
VERSION     DAOTON010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86842_length_1264_cov_3.460711"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="P5292_13450"
     CDS             <1..298
                     /locus_tag="P5292_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010135974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRS40180.1"
                     /translation="GDDEYLGLLRDHLQELLREFSPDFVCYLSGVDVLATDKFGKLKL
                     TRAACQERDKIVFSTFRDRNIPVAVAMGGGYSPDLRTIVEAHCDTFRIARDLYC"
     gene            330..>1264
                     /locus_tag="P5292_13455"
     CDS             330..>1264
                     /locus_tag="P5292_13455"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRS40181.1"
                     /translation="MSVSMTAGWRSFRRYRASQWLAAARLMVAKRIVQAGWYKRRDVS
                     TTRRPGRLFTFRLQQSEYFDGQRFRIDSHDIPFSHGMDWQQSRREALVGIRLRGWSWL
                     RTEGLKEEHALGALLDFIATENGFTYRAEPYELSLRIQHACWWLGYHDRADVIVMQYI
                     ADAAARLRWLTEEHLSNNHLLENGFALCWAGLILDNASYRSRGLDILRTAWQSQVLPS
                     GSHSEQSPMYQHILLARCIETIALMRNCSKETEAGFLVPVVASLAGWLSYLQWRNGAF
                     PVINDSVERPLPEFSELSAALRALGIDQQQPVVAGP"
BASE COUNT          254 a          329 c          384 g          297 t
ORIGIN      
        1 cggagacgac gaatatctgg gtttacttcg cgatcatctg caggaactac tccgggagtt
       61 ctcgccggat tttgtctgtt acctttcggg tgttgatgtg ctggctactg ataagttcgg
      121 gaagttgaaa cttacccgcg cagcttgtca ggagcgcgac aaaatcgtct tctcgacgtt
      181 tcgtgaccgc aacatcccgg tggcagttgc gatgggcgga ggctattcgc ccgacctgcg
      241 aaccatcgtc gaggcgcatt gcgatacttt tcggatcgcg cgagatctgt attgctgagg
      301 gttaacggcc gaggtcgtat tttgtgcgca tgtccgtttc aatgaccgca ggttggcgat
      361 cgttccgccg ctaccgtgcc agccaatggc tcgcggcagc ccgactgatg gtcgcgaagc
      421 gaatcgtaca ggccggttgg tacaaacggc gggacgtttc aacgacgcgt cggccgggca
      481 ggttgtttac gttccggctg caacagtcgg agtacttcga cggacagcga ttccggatcg
      541 actcgcacga cataccgttc agccacggaa tggactggca gcagagcagg agggaagcac
      601 ttgttgggat cagacttcgc ggttggtcct ggttgcgtac cgaaggattg aaggaagaac
      661 acgcgcttgg agcattactg gatttcatcg ccacggagaa tggtttcacg tatcgtgcgg
      721 agccgtatga attgtcgctg cggatccaac atgcatgttg gtggctgggt tatcatgacc
      781 gggccgacgt aatcgtcatg caatacattg ccgatgcagc cgcacgattg cgttggctga
      841 cggaagagca cttatccaac aatcatttgc tcgaaaacgg tttcgccctg tgttgggcgg
      901 gcttgatcct ggacaatgcc tcgtatcgct cccgtggatt ggacatcctg cgaaccgcct
      961 ggcaaagcca ggtgcttcct tcgggtagcc attcggagca atccccgatg tatcagcata
     1021 tcctgttggc ccgctgcatc gagaccattg cgctcatgcg gaattgcagc aaagaaacgg
     1081 aagcaggatt cctggtaccg gttgttgcat ccttggcagg atggttaagt tatctgcaat
     1141 ggcggaacgg tgcttttccc gtaatcaacg acagcgtaga acggccgctt cccgagtttt
     1201 ccgaactgag cgcggctctt cgcgcgttgg ggatcgatca gcagcagccg gtggtcgcag
     1261 gtcc
//
LOCUS       DAOTON010000865         1264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_86894_length_1264_cov_2.021505, whole genome shotgun sequence.
ACCESSION   DAOTON010000865 DAOTON010000000
VERSION     DAOTON010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86894_length_1264_cov_2.021505"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="P5292_13460"
     CDS             complement(<1..576)
                     /locus_tag="P5292_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRS40182.1"
                     /translation="MKPIKLGIIREGKIPRDKRVPFTPEQCQQLRELFPHVTIVVQPS
                     DWRCYTNAEYEAAGIALQEDVSDCDILMGIKEVPKSELIPGKTYLFFSHTIKKQPHNR
                     GLLQHILREKIRLVDYECLVDLNGNRIIGFGRYAGIVGAYNGIMGYGLKYGLFNLKPA
                     HLCHDKKELFRELRKAHMSNLKIVITGGGRVA"
     gene            complement(634..>1264)
                     /locus_tag="P5292_13465"
     CDS             complement(634..>1264)
                     /locus_tag="P5292_13465"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS40183.1"
                     /translation="FYATLFGATPVGNTYTAGIAAARVQHYQQIAASVVNDSASTWNI
                     GEFEPQAGFGYSALGNGLFQFTDQSDNAQSISWDFGDGGVSTQASPSHTYTATGIYTV
                     TQVVSDGCTSDTSVQTVNVSLIGIEEAESVDILVFPNPVADMLRVRFNGQVVPERWAL
                     YTFDGRLAMEGRFTGQADAVLDLSALPAGSYELLLQQGDKRFRKSLFKR"
BASE COUNT          276 a          345 c          366 g          277 t
ORIGIN      
        1 cgccacgcga ccaccgccgg tgatgacgat cttgaggttc gacatgtggg ccttccgcag
       61 ttcgcggaag agttccttct tgtcgtggca gaggtgcgcg ggcttcaggt tgaacagccc
      121 gtacttcagc ccgtatccca tgatgccgtt gtaggcgccc acgatgccgg catagcgacc
      181 gaagccgatg atgcggttgc cgttcaggtc gaccaggcac tcgtaatcga ccagccggat
      241 cttctcccgc aggatgtgct gcagcaatcc gcggttgtgc ggttgcttct tgatggtgtg
      301 tgagaagaag aggtacgttt ttccggggat cagctccgat ttaggaactt ccttgatacc
      361 catcaggata tcacaatccg aaacatcctc ctgtaaagca atgccggctg cctcgtattc
      421 ggcgttggtg taacaacgcc agtcgctggg ttgcaccaca atggtgacat gcggaaaaag
      481 ctcccgcagt tgttggcatt gttcgggagt aaacggaacg cgtttgtcgc gggggatctt
      541 gccctcgcgg ataataccca acttgatcgg cttcatacgt actggaaagg ggcctgcaaa
      601 ggtacgccct tccgaagcag gaaaaaagcc ggcctatcgc ttaaaaaggc tctttcgaaa
      661 tcgtttatca ccctgttgaa gcagcaattc gtacgagcct gccggtaagg cgctcaggtc
      721 cagtaccgca tcggcttgcc cggtgaacct gccttccatg gccaatcgcc cgtcgaaggt
      781 gtagagcgcc cagcgttccg gtactacctg cccgttgaaa cgtacccgaa gcatgtcagc
      841 aaccggattc gggaaaacaa ggatgtcgac ggattcggct tcctcgattc cgatcaggga
      901 aacattgacg gtttgaacgg aggtatcgga agtgcagccg tcactcacca cctgggtgac
      961 cgtatagatt ccggttgcgg tatacgtatg cgacggactt gcctgagtgg aaacaccgcc
     1021 atctccgaaa tcccaggata tcgattgcgc gttgtcggac tgatcggtaa actggaagag
     1081 gccattgccc aaagcgctgt agccgaagcc ggcctgtggt tcaaactccc cgatgttcca
     1141 ggtggaagca ctgtcgttca cgacagaagc tgcgatctgc tggtagtgtt gaaccctggc
     1201 cgcggcaatg ccggcagtat aggtattccc gacaggagtc gctccgaaca gggtggcata
     1261 gaat
//
LOCUS       DAOTON010000866         1260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_87284_length_1260_cov_3.205809, whole genome shotgun sequence.
ACCESSION   DAOTON010000866 DAOTON010000000
VERSION     DAOTON010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87284_length_1260_cov_3.205809"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="P5292_13470"
     CDS             <1..332
                     /locus_tag="P5292_13470"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS40184.1"
                     /translation="AVLEDRPIGTVAVIPDSPSSVEVAKMGVDERCRGLGAGNALLDA
                     ALRWSVSRNFTTIHLDTADQLVEAIRLYERAGFRRKGDHHVHPVFKRTTFRMELAPEA
                     LERYRSA"
     gene            complement(329..>1260)
                     /locus_tag="P5292_13475"
     CDS             complement(329..>1260)
                     /locus_tag="P5292_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRS40185.1"
                     /translation="PAALDNRPLKFEEFETLVNQVIYVSATPAEYELQQSEGVLVEQV
                     IRPTGLLDPIIEVRPAINQVDDLLEEIDRTIKAGDRVLVTTLTKRMAEELTKYLTNLN
                     INCRYIHSEVETLERVELLRDLRLGNFDVLVGINLLREGLDLPEVSLVAVLDADKEGF
                     LRSNRSLTQTAGRAARNVNGRVIFYADKITESMQQTMDETNRRREKQLRYNEEHGITP
                     TQIRKSKDAILLQTKVADRRQLAEPGAYTEDFATPSIAADPVVQYMDQEQLKKLIGQT
                     RKSMEKAARELDFIEAARLRDELFQLEKMVNAH"
BASE COUNT          246 a          349 c          346 g          319 t
ORIGIN      
        1 tcgcagtact ggaggaccgt cccatcggca cagtagccgt aattcctgat agtccttcaa
       61 gtgttgaggt agcgaaaatg ggggttgatg aacggtgtcg cggcttaggt gccggcaacg
      121 ccttgttgga cgcggctttg cgctggtccg tcagtcgtaa ttttaccacc atccatctcg
      181 ataccgcgga ccagttggtc gaagcgattc ggctttatga gcgtgcggga ttccggcgta
      241 agggtgatca tcatgtccat ccggttttca aacgaaccac cttcaggatg gaattggctc
      301 cggaagcact tgaacgatat cgttccgcct aatgggcgtt caccattttt tccaattgga
      361 ataactcgtc ccgtaaacgg gccgcctcga tgaagtccag ttcacgtgct gctttctcca
      421 tggatttcct ggtctgcccg atgagtttct tcaattgctc ctgatccatg tactgcacga
      481 caggatcagc ggcgatcgat ggggtggcaa aatcttcggt ataggcaccg ggctccgcaa
      541 gctgccggcg gtcggccact ttggtctgca gtaatatcgc gtctttcgat ttgcggatct
      601 gcgtcggtgt gatcccatgt tcctcgttat accgcagctg tttttcccgc cgacgattgg
      661 tctcatccat cgtctgctgc atgctctcag tgatcttgtc agcatagaag attacccgcc
      721 cgttgacatt gcgcgcggcg cgtcctgccg tctgggtgag cgagcggttg ctgcgcagaa
      781 agccctcctt gtcggcgtcc aggaccgcga ccagagaaac ctcaggcaag tcaagacctt
      841 cccgcaacag gttaataccg acgagtacat caaagttgcc aagccgcagg tcgcgcagca
      901 actccacgcg ttccagggtc tccacttccg agtggatata gcggcagttg atgttcaggt
      961 tcgtgaggta cttggtgagt tcctcggcca ttcgtttcgt gagagtggta acgagcacac
     1021 gatctccggc tttgatcgtc cggtcgatct cttcgagtaa gtcgtcgacc tgattgatcg
     1081 ctggccgaac ttcaatgatg ggatcgagca aaccggtcgg ccggatgacc tgttccacca
     1141 gcacaccttc gctttgctgc aattcatatt cagccggggt ggcactcaca tagatgacct
     1201 ggttcaccag ggtttcaaat tcctcaaact tcagcggccg gttatccagc gcggctggta
//
LOCUS       DAOTON010000867         1259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_87392_length_1259_cov_3.382890, whole genome shotgun sequence.
ACCESSION   DAOTON010000867 DAOTON010000000
VERSION     DAOTON010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87392_length_1259_cov_3.382890"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1179)
                     /gene="mqnC"
                     /locus_tag="P5292_13480"
     CDS             complement(55..1179)
                     /gene="mqnC"
                     /locus_tag="P5292_13480"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HRS40186.1"
                     /translation="MNADVLLNRALRLEFLSAEEGQYLFLHAPTAELMDVAHQIRKKL
                     HPDNKVTWIIDRNVNTTNVCIANCKFCNFYRIPGHADAYITTIDQYKEKIEETFRYGG
                     EQLLLQGGHHPGLGLQFYIDTFRELKRLYPNLKLHALGPPEIAHITKLEGTSHTEVLR
                     ALMEAGLDSLPGAGAEILSDRVRRLISKGKCSGREWLDVMRAAHQLHLTTSATMMFGH
                     VETLEERFQHIIWLREVQSEKPADAKGFVSFIPWPFQDHDTILRRIKGVRNEVTGEEY
                     IRTIAISRILLPNIPNIQASWLTVGKEIAQICLHGGANDFGSIMIEENVVSAAGAPHR
                     FTSNGIQQAIREAGFEPQLRNQQFEDRQLPETIEQQLITY"
BASE COUNT          277 a          308 c          389 g          285 t
ORIGIN      
        1 gcaccagcgc ggcagaaagg aaaattcgaa gcagtgcggg acgcatgcgg agcatcagta
       61 ggtgatgagt tgctgttcga tggtttcggg taattggcgg tcctcgaact gttggttacg
      121 aagttgcggt tcgaatccgg cctcacgaat agcctgttga atgccatttg acgtgaagcg
      181 atgcggtgcg ccggcggcgg aaaccacgtt ctcttcgatc atgatggagc cgaaatcatt
      241 ggcgccgcca tggaggcaga tctgcgcgat ctccttgccg accgtcagcc agctcgcctg
      301 gatgttcgga atattcggca gcaggatgcg actgatggcg atggtgcgga tatactcttc
      361 gccggtcact tcattgcgta cgcctttgat gcgacggagg atggtatcgt ggtcctggaa
      421 gggccaggga atgaacgaga caaaaccctt cgcgtccgct ggcttctccg attggacttc
      481 acgcagccag atgatgtgct ggaagcgttc ttccagcgtc tccacatggc cgaacatcat
      541 ggtggcggaa gtcgtgagat gcagttggtg cgcggcgcgc atgacatcga gccattcgcg
      601 accggagcat ttccctttgc tgatcagccg gcgaacacga tcgctcagga tctcggcgcc
      661 ggcgccggga agtgaatcaa gtccggcttc catcagggca cgaagcacct cggtatgcga
      721 tgtcccttcg agcttggtga tatgcgcgat ctcgggaggc ccgagggcat gcaacttcag
      781 attcggataa agtcgtttga gttcccggaa agtgtcgata taaaattgta aaccaagtcc
      841 ggggtgatgt ccaccctgca acaagagttg ctccccgcca tagcggaagg tttcttcgat
      901 cttttctttg tactgatcga tggtggtgat gtaagcatcg gcatgtccgg ggatccggta
      961 gaagttgcaa aacttgcaat tcgcaatgca gacattcgtg gtattgacat tccggtcgat
     1021 gatccaggta accttgttat cgggatgcaa ctttttccgg atctggtggg caacatccat
     1081 caactccgcc gtgggagcat ggaggaacag gtactgccct tcttccgcgg agaggaattc
     1141 gaggcggagg gcgcggttta ggagaacgtc ggcattcatg gtaaagcggt gaacgggaac
     1201 acgttcggtt agagaacaac tgaaacgtct cctggttcca cgggaaagag cggaaaaat
//
LOCUS       DAOTON010000868         1259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_87420_length_1259_cov_2.436877, whole genome shotgun sequence.
ACCESSION   DAOTON010000868 DAOTON010000000
VERSION     DAOTON010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87420_length_1259_cov_2.436877"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1247
                     /locus_tag="P5292_13485"
     CDS             189..1247
                     /locus_tag="P5292_13485"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRS40187.1"
                     /translation="MEKPKPKSEPAIDMTSQVVQSATPATSAVDQEFPENRVDKLDKG
                     VLAIMQDRKNHYWFGTNEGAFRYDGKSLIVFTEKDGLRQNQVRQIQEDASGRIWFSTG
                     GYGVSYFDGKKIYSASDNYTSGSGKDWTIGKDDSWFPAGGGAYRYHDQSMTYLPFPEV
                     TANASTSRQAPTQLSAYGVYSLLKDRNGNWWFGTQARGVCRFDGKSFTWFTGSGLKGP
                     AVLALFESRDGTLWFGNNGRGLFRYDGKDLTNVTEEKGLANPEFIRNGRVLPGTLARV
                     LSLNEDNAGNLWIGTADAGVWRYDGESLINYTPADGLPDCAIETIYLDKQGQLWFGTN
                     GDGVYKFNDRRFSKALFN"
BASE COUNT          331 a          269 c          344 g          315 t
ORIGIN      
        1 atcgtgtctc aatgcatgaa cagagaaaat gatctaaagc aatgtctgtt acaccgcttg
       61 acctcaagtg ccggcaatta cttttttaat gtacacttgt gtaatctttt attgctaaaa
      121 atgtgtcgga aatgatgagt gttcggattt ttatttcgag ttgctgtttg attttgatca
      181 gctcgtgtat ggagaaacct aaaccaaaga gcgaaccggc aatcgatatg accagtcagg
      241 ttgttcaatc tgctactccg gctacaagcg ctgttgatca ggagttcccg gaaaaccggg
      301 tggacaagct tgacaaaggc gttcttgcca tcatgcagga tagaaagaat cactattggt
      361 tcggtacaaa cgaaggcgca tttcggtacg atgggaaatc attgattgtg tttactgaga
      421 aagacggact gcgtcaaaac caggtacgac aaatacagga ggatgcttcc ggaagaatct
      481 ggttttccac gggaggatac ggagtcagtt atttcgacgg taagaaaatt tattccgcgt
      541 cggataacta tacctccggc tccgggaaag attggaccat cgggaaagac gattcatggt
      601 tccctgccgg cggaggcgca taccgatacc atgatcaatc catgacctac ttgccattcc
      661 cggaggtcac ggctaacgca tcgacatccc ggcaagcccc gacgcaatta agcgcctacg
      721 gcgtctattc gctcctgaag gaccggaatg gaaattggtg gtttggcacc caggcgcggg
      781 gcgtttgtcg gttcgatgga aagtcgttca cctggttcac cggatcgggt ttgaaaggtc
      841 cggctgtcct ggccctattc gaatcccggg acggtaccct ctggtttgga aataatggaa
      901 gaggattgtt ccgttacgat ggaaaagacc tgacaaacgt taccgaagag aaagggctgg
      961 caaatcctga atttattcgg aatggtcgtg tcctgccagg tacactggcg cgggtcttgt
     1021 cgttgaatga agataatgcc ggtaacctgt ggatcggaac ggcggatgcc ggtgtatggc
     1081 gatatgacgg agaaagcctg atcaattata caccagcgga tgggttaccc gattgcgcga
     1141 ttgaaacgat ttatctggac aagcagggac agctttggtt tgggacgaat ggtgacgggg
     1201 tatataaatt caacgaccgt cgtttttcga aggccctttt caattaggga aaagcatac
//
LOCUS       DAOTON010000869         1257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_87626_length_1257_cov_2.649750, whole genome shotgun sequence.
ACCESSION   DAOTON010000869 DAOTON010000000
VERSION     DAOTON010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87626_length_1257_cov_2.649750"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1257)
                     /locus_tag="P5292_13490"
     CDS             complement(<1..>1257)
                     /locus_tag="P5292_13490"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS40188.1"
                     /translation="DITVCANAQTCFDIFSSDPDAGQNLTVTWDDAIPGATFTTSSGP
                     RPTATFCWTPTSAEIGNSYTFTATVRDDACPYYGSQTYAYTIHVIGISVNAGPDQSIA
                     CSDLATLTANASGGGPYTYLWNNGSTMQSITVGAGTWWVTASNGTCTATDTVVVTMPF
                     IPVADFTHSATSCLNTPIQFTDQSTTPGGIIFSWIWDFGDGSGSTQQNPTHQYSTPGT
                     YDVSLVIENSLGCTDTVTQTIVIAPPPVAAFTATSACVNSNVSFTDQTSPAGTSWNWT
                     FGDGGSSTLQNPTHTYASPGTYNVTLVSTSAGGCIDTVVNPVTVFPLPAINAGADQTI
                     CAGASTTLSASGGTTYTWNPGGNTGGTITVSPTTGTTYVVIGTDVNGCSISDTIRVSV
                     TALPRIAAGADVVICAGNAATLNASGG"
BASE COUNT          258 a          318 c          416 g          265 t
ORIGIN      
        1 ccgccgctgg cgttcagcgt ggcggcgtta cctgcgcaga ttaccacgtc ggcgccggcg
       61 gcgatgcggg gtagcgccgt taccgaaaca cggatcgtgt cactgatcga gcagccattg
      121 acatccgtgc cgatgaccac gtaggttgtt ccggtcgtcg ggctcaccgt aatggtgcca
      181 cccgtgtttc ctcccggatt ccaggtatag gtcgtgccac cggaggcgct cagtgtcgta
      241 cttgcgccgg cacaaatggt ctggtcggca ccggcattga ttgccggaag cgggaacacc
      301 gttaccgggt taacgaccgt gtcgatacag ccgccggcgc tggtggatac cagcgtaaca
      361 ttgtacgtgc cgggagaagc gtaggtatgg gtggggtttt gaagggtgga acttccgcca
      421 tcaccgaatg tccagttcca ggaagtgccg gcaggactcg tctggtccgt gaagcttacg
      481 ttgctgttga cgcaggcgct ggtcgcggta aaggccgcta ccggtggcgg ggctatgaca
      541 atcgtttgcg tcaccgtgtc ggtgcatccc aacgagtttt caatgaccag ggagacatca
      601 taggtgcccg gagtcgaata ttggtgggtt ggattttgct gcgtacttcc gctgccgtca
      661 ccgaaatccc agatccaact gaagataatg ccaccgggag tggtggattg atcggtaaat
      721 tgaatggggg tattcagaca gctcgtggcg gaatgtgtaa agtcggctac cgggatgaag
      781 ggcatggtaa ccaccacggt gtcggttgcg gtacaggtac cattcgatgc ggttacccac
      841 caggttccgg cacccacggt aatggactgc atcgtcgaac cgttgttcca caagtaggtg
      901 taaggaccgc cgccgcttgc attggcggtc agcgtggcga ggtcggaaca ggcgatcgac
      961 tggtccggac cggcattcac ggagataccg atcacatgga tggtataggc ataggtctgt
     1021 gaaccgtagt acggacaagc gtcatcgcgt acggtagcag tgaacgtata gctgttgccg
     1081 atttctgcac tggtaggtgt ccagcagaaa gtggcggtag gccttgggcc ggaggaggtg
     1141 gtaaaagtcg caccgggaat ggcatcgtcc caggtgacag tcagattctg accggcgtcg
     1201 ggatcagacg agaagatatc gaagcaagtc tgcgcattgg cgcaaacggt gatatcg
//
LOCUS       DAOTON010000870         1255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_87844_length_1255_cov_4.280833, whole genome shotgun sequence.
ACCESSION   DAOTON010000870 DAOTON010000000
VERSION     DAOTON010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87844_length_1255_cov_4.280833"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="P5292_13495"
     CDS             <1..439
                     /locus_tag="P5292_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRS40189.1"
                     /translation="AKALCKNTNTPVARMIEKGLMRLGKPVKEIEGAIENVAKLEIYK
                     MENNMNVLGTISGIAPMFGFLGTIFGVIKIFYQIALQNSLEINAISGGLYVKMISSAA
                     GLLVGMIAYACYHYLVYMIDRVINKMEINAVQFIDLLEEPVKG"
     gene            473..877
                     /locus_tag="P5292_13500"
     CDS             473..877
                     /locus_tag="P5292_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRS40190.1"
                     /translation="MNFRRRNRLVGEVYTASLNDIMFFLLLFFLITSTLATPNVLKLL
                     LPSAKSSSQSVKHPLTISVTADLQYAINNTPVPADQLEAAIEQAIKGQPDPTALLKVD
                     KTVQVQNLVDLLDIGNRKKVKMVLATQTSKQQ"
     gene            878..>1255
                     /locus_tag="P5292_13505"
     CDS             878..>1255
                     /locus_tag="P5292_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40191.1"
                     /translation="MATEAGIRSRSILISVLVHGLLFLILLFTVMSTPIPPFPETGGG
                     GGVIVNIGYVDEASGEVQPMSPNTTEDPNLSASVPVPSPDEQFATQEIEESPIAKEKP
                     DPAKTSPKTPRLTTPKTQPVKTEP"
BASE COUNT          316 a          369 c          318 g          252 t
ORIGIN      
        1 cgccaaggcg ctctgtaaaa acaccaacac cccggtggcc cgcatgatcg agaagggcct
       61 gatgcgcctc gggaaaccgg tgaaggagat cgaaggagcc atcgaaaacg tggccaagct
      121 cgaaatctac aagatggaga acaacatgaa cgtactgggt accatctccg gtatcgcccc
      181 gatgttcgga ttcctcggaa cgatttttgg cgtgatcaag atcttctatc agatcgccct
      241 gcaaaacagt cttgagatca acgccatctc cggcggtttg tatgtgaaga tgatctcctc
      301 ggcagccggt ctgctcgtag gtatgatcgc gtacgcctgt tatcactacc tggtgtacat
      361 gatcgaccgt gtcatcaaca aaatggagat caacgctgtc cagttcatcg acctgctgga
      421 agaacccgtg aaaggataag gtcgcagttc cggatctccg acaaccacta ccatgaactt
      481 ccgcagaaga aaccgactcg tcggcgaagt atacaccgct tcgttgaacg atatcatgtt
      541 ctttctcctg ctcttcttcc tgatcacctc tacgctggca acgccgaacg tactgaagct
      601 cctgcttccg tcggccaaga gcagcagcca gagcgtcaag caccccttga ccatttcagt
      661 gacagcggat ctgcagtatg ccatcaacaa tacgccggtt ccggcggatc agctggaagc
      721 ggcgatcgaa caggccatca aaggccagcc cgacccgacc gcgctgctga aagtcgacaa
      781 gaccgtccag gttcagaatc tggtcgacct gctcgatatc ggtaaccgga aaaaagtgaa
      841 aatggtgctt gccacccaaa cctccaagca gcagtaaatg gctacggaag ccggcattcg
      901 ttcgcgctcc atcctcattt ccgtactcgt acacgggttg ttgtttctca tcctgttgtt
      961 tacggtcatg tcgacaccca ttcctccttt tcctgaaaca ggaggcggag gcggagtgat
     1021 cgtgaacatc ggctacgtgg acgaagcgag tggagaagtg caacccatgt caccgaatac
     1081 cacggaggat cccaatctat cggcttcggt tcctgtccct tcgccggacg aacagttcgc
     1141 gacgcaggag atcgaagagt cgcctattgc caaagagaaa cccgacccgg ccaagaccag
     1201 tcccaagacg ccacgtctga cgacgcccaa gacccagccg gtgaaaacgg aaccg
//
LOCUS       DAOTON010000871         1253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_88142_length_1253_cov_2.044240, whole genome shotgun sequence.
ACCESSION   DAOTON010000871 DAOTON010000000
VERSION     DAOTON010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88142_length_1253_cov_2.044240"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="P5292_13510"
     CDS             <1..651
                     /locus_tag="P5292_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40192.1"
                     /translation="GVFNGELYAGGSFSQSGATPLRGVAKWSGTDWIDLGFGVRYTIP
                     GLMSFVHTFKTYQNSLIVSGGFDQIFYGNDTLNGTAVFAFTGNSVDTLGGGWPGKEVE
                     GVAEFNGQLFVGGGTYLDSYVAHYDLTVGVAAVQQDFRGLSVVRLPDGSLSLTSRQVL
                     ERVRVYDLSYRCLSDDRPLMTHFTFPFSVNGPCVLEVQIKGVPLRKLLLSGYVSEE"
     gene            678..1172
                     /locus_tag="P5292_13515"
     CDS             678..1172
                     /locus_tag="P5292_13515"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRS40193.1"
                     /translation="MHLKQLTDHYIRFNNWANQRLVRWLEQVPVDKLYEKVPSSFPSI
                     DATLQHVLRTQRFWHLFILERDHSTVNWSVRENEAPQILRELEEVSSRMEDDFLTFTE
                     ETLTRKLVLDMPWAKNSLCRYEYIQHIVNHGTFHRGQVVTIARSVGLTSGVPNTDYNI
                     FRCP"
BASE COUNT          280 a          326 c          345 g          302 t
ORIGIN      
        1 ggagttttca acggtgaact ctatgcaggc gggagcttta gtcagagtgg cgcaacccct
       61 ttgcggggtg tcgccaagtg gagcggcacc gattggattg acctcggctt cggggtgcgc
      121 tatacgattc cgggcttgat gagttttgtg catacgttca aaacctatca gaacagcctg
      181 atcgtaagcg gaggattcga ccagatcttc tatggcaatg acactttgaa cggtactgcc
      241 gtgttcgcct tcaccggtaa ctcggtggat acgctgggcg gtggctggcc gggcaaagaa
      301 gtggaaggag tggcggagtt caacgggcaa ttattcgttg gcgggggcac ctatctcgat
      361 tcctatgttg cccactatga tctgacggtg ggagttgcag ctgtacagca ggatttccgc
      421 ggcttgtccg ttgtccgttt accggatggt tcgcttagcc ttacctcacg gcaagtgctc
      481 gaacgagtac gcgtgtacga cctgagttat cgctgccttt cggatgaccg cccgctcatg
      541 acccacttca cctttccctt ttcggtaaat ggtccttgtg tgctggaagt acagatcaag
      601 ggcgtgcccc tgcgcaagct cctgctttcc ggctatgtca gtgaagaata ggacaatcat
      661 actatccttg atttcttatg cacctgaaac aactcaccga tcactacatc cgcttcaaca
      721 actgggctaa ccaacgtttg gtgcgttggc tggagcaggt tcctgtggac aagctgtatg
      781 aaaaagtgcc gtcgagcttt ccgagcatcg atgccaccct gcaacacgtc ttacgcaccc
      841 agcggttctg gcatttgttc attctggaac gggatcattc tacggtgaac tggagcgtac
      901 gcgaaaacga agcaccgcag atccttcggg aactggagga ggtatctagc aggatggaag
      961 atgattttct gacgtttaca gaggagacct tgacgcggaa actggtgctg gacatgccct
     1021 gggcgaagaa cagcctttgt cgctatgagt atatccaaca catcgtcaac cacgggacct
     1081 ttcatcgcgg acaagtggtg acgatcgcgc gaagtgtggg cctgaccagc ggcgtgccga
     1141 ataccgatta caacattttc cgttgtccgt aaccgaatcc tattcgatac catcctccat
     1201 ggctaagaac aagaccgaac ccactcaggc aagcgtcgaa cgattcatcc gca
//
LOCUS       DAOTON010000872         1248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_88648_length_1248_cov_3.412406, whole genome shotgun sequence.
ACCESSION   DAOTON010000872 DAOTON010000000
VERSION     DAOTON010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88648_length_1248_cov_3.412406"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="P5292_13520"
     CDS             complement(<1..511)
                     /locus_tag="P5292_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40194.1"
                     /translation="MKRKLYLTFFSAILALPLLAQPTLTTADLPVAGLAFTMVQDSTY
                     NAPIPSGGANVTWDYSGLQNLLIDTIGFGAAAGTPYAGSFPTSNLASQADNLTDWTYT
                     TNNSTGFYLDGVGTPTLTIRYNPPSLFAPVPFTYNQTRTNIARIVQDTVIIDTTGTPR
                     NVRFIQNQRG"
     gene            complement(611..>1248)
                     /locus_tag="P5292_13525"
     CDS             complement(611..>1248)
                     /locus_tag="P5292_13525"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRS40195.1"
                     /translation="LLASLLLFFPFLSLAQNGSDAKSQEVLKSVSAKYKSFKSLKASF
                     TVTIESPGKAKEVQKGTLYIKGDKYRLEFAGQDVISDGKTRWTYVKDANEIQIDNQRK
                     DENAITPTNIFTLYEKGWNSKYIGEAKEPSGPAHQIELVPIEPKKKNVFKVKIAVSKA
                     DKFIVSAKVFDKNGGFQTFQVDKLSPDILTDDTQFVYNATRYPGAEVVDLR"
BASE COUNT          296 a          284 c          329 g          339 t
ORIGIN      
        1 cgcctcgctg gttctggata aaccggacgt tacgaggcgt accggtcgta tcgataatta
       61 ccgtatcctg tacgatgcgc gctatgtttg ttctggtttg gttataagtg aacggaaccg
      121 gagcgaaaag ggaaggagga ttgtaacgta tggtcagcgt cggggtacct acgccatcaa
      181 gataaaaacc tgtgctgtta ttggtcgtgt aggtccagtc ggtaaggtta tcggcttgcg
      241 atgcaaggtt actggttgga aacgaccctg catagggcgt gccggcagca gccccgaagc
      301 caatcgtgtc gatcaacaga ttttgcaaac cggaataatc ccaggtcacg tttgctccgc
      361 ccgaagggat gggagcattg taggtggaat cctgcaccat cgtaaacgca agtccggcca
      421 cgggcaagtc tgcagtagtc agggtgggtt gggccagcaa cggaagtgcg aggatggccg
      481 aaaagaatgt caggtagagc ttccttttca tgctttgtta ttttacataa aagtagttgt
      541 tccgaattga tgctgatcgt gaaaatgcaa aaaggggctt aggccccttt ttgattgaga
      601 aaatacaccg ttatcggaga tcgactacct ccgcacccgg atatcgggtc gcgttataga
      661 cgaattgagt gtcatcggtt aaaatatccg gcgatagttt gtcgacctgg aaagtctgga
      721 atccaccatt cttgtcgaac acttttgccg aaacgataaa cttgtcagcc ttgctaacgg
      781 cgatctttac tttgaagacg ttctttttct tgggttcgat cggtactaac tcgatctggt
      841 gggccggccc cgaaggttct ttcgcttcac cgatgtactt ggaattccat cccttttcgt
      901 acaaggtgaa aatgttggtc ggcgtgatgg cgttctcgtc cttacgttga ttatcgatct
      961 ggatctcatt cgcatctttc acataggtcc agcgggtctt gccatccgag ataacatctt
     1021 gtcccgcgaa ttccagccgg tacttatctc ctttgatata caacgtccct ttttgaacct
     1081 ctttcgcttt tcccgggctt tcaatcgtca cggtgaaact ggccttaagg gatttgaagg
     1141 acttatactt cgcggaaacc gatttgagga cctcctgtga tttcgcatcg ctgccatttt
     1201 gagcaaggct caaaaaagga aaaaaaagca gaagcgaggc gagtagtg
//
LOCUS       DAOTON010000873         1248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_88662_length_1248_cov_2.825650, whole genome shotgun sequence.
ACCESSION   DAOTON010000873 DAOTON010000000
VERSION     DAOTON010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88662_length_1248_cov_2.825650"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="P5292_13530"
     CDS             <1..946
                     /locus_tag="P5292_13530"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRS40196.1"
                     /translation="TRQPCAEKGGTFVQRYNLTVSDLVFIIGPRINAAGRIHDARHAV
                     NLLISDNSADALANSITVNDHNTERKNLDVKITQEAMEIIEGDAQFAKRKATLLFRPD
                     WHKGVIGIVASRLTEKFYRPTIVLTQSGDDIITGSARSVKDFDIYNAIESCSDLLEQF
                     GGHMYAAGLTLRKENLEKFSERFEEVVAATIQDHMLVQEIEIDADLRLTDITSSFFNV
                     LKQFAPFGPGNMNPVFRTENVRDTGLSRIVGNNHLKLNLAEDETTRFGMDGIAFQLGQ
                     FYPFISRRIPFDICYTIEENTFNGKTTIQLNVKDIKVH"
     gene            complement(965..>1248)
                     /locus_tag="P5292_13535"
     CDS             complement(965..>1248)
                     /locus_tag="P5292_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018474886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRS40197.1"
                     /translation="GVHVDGAITAIIVAAVLAFLNAIVKPILVLLTIPITVVTLGLFL
                     LVINALMILAAQKIVPGFSVDGFWIALLFSIVLSVVNAVFEGIAGSSKN"
BASE COUNT          311 a          379 c          306 g          252 t
ORIGIN      
        1 cacccggcag ccctgcgcgg aaaagggagg aacttttgtt caacggtata atctcacggt
       61 cagcgacctg gtgttcatca tcgggccacg catcaatgcc gccggtcgta tccacgatgc
      121 gcgccacgcc gttaacctgc tgatctccga caactccgcc gacgccctgg ccaacagcat
      181 cacggtcaac gatcacaata cggagcggaa aaacctcgac gtcaagatca cccaggaagc
      241 gatggagatc atcgaaggcg atgcccaatt cgccaagcgg aaggcgaccc tgctctttcg
      301 ccccgactgg cacaaaggcg tcatcggtat cgttgcttct cgcctgacgg aaaaattcta
      361 ccgccccacg atcgtcctga cccagtccgg cgacgatatc atcaccggct ccgcccgttc
      421 cgtcaaggat ttcgatatct acaatgccat cgaatcctgc tccgacttgc tcgaacagtt
      481 cggcggccac atgtacgcgg ctggtctcac cttgcgtaag gagaacctcg aaaaattctc
      541 cgaacgattc gaagaggtcg tcgcagccac gatccaggac cacatgctgg tacaggagat
      601 cgagatcgat gccgacctgc gcctgaccga catcacgtct tcatttttta atgtcctcaa
      661 gcagtttgca ccctttggtc ccggtaatat gaatccggta ttccggacgg aaaatgtgcg
      721 cgataccggc ctctcacgca tcgtcggtaa caaccatttg aaattgaacc tggcggagga
      781 cgaaacgacc cgtttcggta tggatggcat cgccttccag ctcggacagt tctatccgtt
      841 catctcccgc cggatcccct tcgacatttg ttataccatc gaagaaaaca cgttcaatgg
      901 caagactacc attcaactga atgtgaagga cattaaagtc cactgatccc gtaagattct
      961 acgcttaatt tttcgagctg ccggcgatgc cttcgaacac ggcattcacg accgacaaca
     1021 cgatcgagaa caacaaggcg atccagaaac cgtcgaccga aaaacccggt acgatctttt
     1081 gcgcggccag gatcatcagc gcgttgatga ccagcaggaa aagtcccagg gtgaccaccg
     1141 taatcggaat ggtcagcagc accaggatcg gcttgacgat cgcgttcaga aatgcgagga
     1201 cggccgccac aatgatggct gtaatggccc cgtcaacatg tactccgg
//
LOCUS       DAOTON010000874         1242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_89357_length_1242_cov_1.936816, whole genome shotgun sequence.
ACCESSION   DAOTON010000874 DAOTON010000000
VERSION     DAOTON010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89357_length_1242_cov_1.936816"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..>1242
                     /locus_tag="P5292_13540"
     CDS             295..>1242
                     /locus_tag="P5292_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRS40198.1"
                     /translation="MMKEGWWQRLQRTRLVRYTNTYLKNRYDKAIQTRSRLKLQFLQF
                     FPFIVAATITGVFAYAYSRIFHYAELFSHTLLAYNRYAIFVVTPVCFLASWWLVWRYA
                     PFARGSGIPQVMASIELSQRGRNPFVDQLLSWKIILVKVISSTIKVIGGGILGREGPT
                     IQVAAAIFKLVHDRLPKWWTSVTASNVILAGGAAGLAAAFNTPLGGIVFAIEELSKHH
                     IRFYKSALFIAVIISGLAAQGLGGGYLYLGQAATNYDGWWIYLGIILVAIVTGYFGSV
                     MCSVILRIMKLMRRRRGSTTELLMVLGGAFFVAFFIFLIG"
BASE COUNT          255 a          309 c          361 g          317 t
ORIGIN      
        1 tcgacattta cctgcccgaa tggctggcga agtataacaa ggagatctat acgacaattg
       61 tggtgggcat cgccattgct acggttgtcc gggtgatgaa cggatgaagc atccggttcc
      121 tgcctgcgca agtggatgat tgcattgtta aggcttacgg attgacggtt tcatcctgtt
      181 atcgactggt tcaccttggc acttctttct tatattcgtt caaccgtagg gctgcatgga
      241 taaaggcgcc gatcaccgga aggagtcgga tgccgcccca tccaatttga aggcatgatg
      301 aaagaaggtt ggtggcaacg attacaacgt acccgtctgg ttcgttacac caacacgtac
      361 ctgaagaacc gatacgacaa ggcgatccag acccgctcca ggctgaagct gcagttcctg
      421 cagttctttc cattcatcgt agccgccacg atcaccggtg tattcgcgta tgcttacagc
      481 cggatcttcc actacgccga gttgttttcg cacacactac tggcctacaa ccggtacgcg
      541 atcttcgttg tcacgccggt ttgtttcctg gcgtcctggt ggctggtgtg gcgctatgcg
      601 ccatttgccc gcggaagcgg aattccgcaa gtgatggcct cgatcgaatt gagtcagcgc
      661 ggcaggaacc cgttcgtaga tcaattgctg agttggaaga tcatcctcgt gaaagtgatc
      721 tccagtacca tcaaagtcat cggcggtgga atactcggtc gagagggtcc gaccatacag
      781 gtcgcagcgg cgatcttcaa gctggtgcac gaccggcttc cgaaatggtg gacatccgtt
      841 accgcgagca acgtgatcct ggcgggcggg gcagccggct tggctgcggc attcaatacg
      901 ccgctcggtg gaatcgtctt tgcgatcgag gaattgtcga agcatcacat ccgattctat
      961 aagtcggctt tgttcatcgc ggtgatcatc tcgggtcttg cagcccaggg attgggagga
     1021 gggtatctgt atctcgggca ggctgccacc aactatgacg gttggtggat ttatctcggg
     1081 atcatactcg tggcgatcgt gacggggtac ttcggaagcg tcatgtgctc agtgatcctg
     1141 cgcatcatga aactgatgcg gagacggcgg gggagtacta cggaattgtt gatggtgtta
     1201 ggtggcgcgt tcttcgttgc ttttttcatt ttcttgattg ga
//
LOCUS       DAOTON010000875         1240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_89529_length_1240_cov_2.679325, whole genome shotgun sequence.
ACCESSION   DAOTON010000875 DAOTON010000000
VERSION     DAOTON010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89529_length_1240_cov_2.679325"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..216)
                     /gene="rpoZ"
                     /locus_tag="P5292_13545"
     CDS             complement(100..216)
                     /gene="rpoZ"
                     /locus_tag="P5292_13545"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRS40199.1"
                     /translation="MLDADRDKPTVIALREVAAGKVGIEMLVKAPPTPILSG"
     gene            215..331
                     /locus_tag="P5292_13550"
                     /pseudo
     CDS             215..331
                     /locus_tag="P5292_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902977.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
     gene            343..1098
                     /locus_tag="P5292_13555"
     CDS             343..1098
                     /locus_tag="P5292_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40200.1"
                     /translation="MKNHGLLLLALNCLLIPNTVPAQATFQPTTFSGLAAQEPISLVI
                     EQVGLRSIEGRLTDSQGTYPYTATVAGDSILHGELTIQQFQLAFSGKLTDRSNLRLNF
                     LAPDGSGAVSSVVLKEDVKSSQPVLAQPNAAHDSRLVGTWTSTVTETSGWGESLSILS
                     YVLVDVFYADGTYGSGKGDMQTMNSDYHGYDENIHVEKQPNVSWSTQGSTLYLDLLTE
                     NGPQKMQGSYAIIGDKLRITWSDGNEVELTRRK"
BASE COUNT          276 a          352 c          324 g          288 t
ORIGIN      
        1 acgcgcgctc gaccagggcg acgtcgggcg ggccgaggta gtggcccagg tgccgggtga
       61 actcggcgat ctccgacatg cccgggatgc cgggggcgct taccccgaca ggatcggcgt
      121 cggcggcgcc ttgacgagca tctcgatgcc gaccttgccc gccgcgacct cgcgcagcgc
      181 gatcaccgtc ggcttgtcgc ggtcggcgtc gagcatcggc gtcgacccgg ccgtctcgtc
      241 cgcaccgttc atcgcaacat tggtcgacgt tacagggctt gttatttact tcgggttcgc
      301 ttacctgctg ttgaagggtt cgatgcttta agtcgttgac gtatgaaaaa tcatggtttg
      361 cttttactag ccctgaactg tctgttgata cccaatactg ttcctgctca ggctacgttc
      421 caaccaacca ccttctccgg acttgcggcg caagaaccca tctccctggt aattgaacag
      481 gttgggttgc gctccatcga gggcaggcta accgattctc aaggcactta tccgtacacc
      541 gccacggttg caggcgactc tattcttcac ggcgaactca ccattcagca gtttcagctt
      601 gccttttccg gtaaactgac tgatcgatcc aacctgcggc ttaacttcct ggcacccgat
      661 ggttccggtg ctgtttccag cgtggtgttg aaagaagatg tcaagagcag tcagcccgtc
      721 ttggctcaac cgaacgctgc gcacgattcc cgtttggtcg gaacctggac atcaactgtt
      781 accgagacgt ccggctgggg agaaagcctt tccatattat catatgtact ggtagatgtg
      841 ttttacgcgg atggaacata cggatcaggg aagggcgaca tgcaaaccat gaattcggat
      901 taccacggat atgatgaaaa catccacgtt gaaaagcaac cgaacgtaag ctggtcgaca
      961 caaggtagca cgctctacct tgaccttctg actgaaaatg gcccgcaaaa aatgcaggga
     1021 agctatgcta tcataggcga caaactgcga attacctgga gtgacggaaa tgaagtggaa
     1081 ttaaccagaa gaaaatgaaa cggacctttt ctgtcttcat cctttcactc gtactttttg
     1141 cctgcggatc cgatcagtcg aaacaactgg aaagcgagct cctacccggc ccttcgcttc
     1201 ctacattgac ggaaaccttt accggtatcc tcccctgcaa
//
LOCUS       DAOTON010000876         1240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_89541_length_1240_cov_2.333333, whole genome shotgun sequence.
ACCESSION   DAOTON010000876 DAOTON010000000
VERSION     DAOTON010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89541_length_1240_cov_2.333333"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="P5292_13560"
     CDS             <1..547
                     /locus_tag="P5292_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRS40201.1"
                     /translation="MKPGAILINASRGTVVEIEPLAQALRDKKLLGAAVDVFPVEPKS
                     NKDEFQSPLRGLDNVIITPHVGGSTQEAQANIGLEVAEKLVRYSDNGTSTTSVNFPEV
                     ALPAHPGKHRILHIHANRPGVLGEINSKLSKLNVNILGQYLQTNDRIGYVVTDMDKRT
                     SSKALEALKKVKHTIRVRNLY"
     gene            547..1014
                     /locus_tag="P5292_13565"
     CDS             547..1014
                     /locus_tag="P5292_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRS40202.1"
                     /translation="MANLQPFIIRPARAADVPAMFALVKELALFEKAPEQVTNSEADM
                     LRDGFGEQPVFRSLVAECEGQVVGLAIFFVKYSTWKGKGLYLDDLIVTETYRGRGIGL
                     ALLKAYLEEARRSGARQVHWQVLDWNQPAIDLYKKMGASLEAEWLDCKMNVGE"
BASE COUNT          332 a          332 c          341 g          235 t
ORIGIN      
        1 catgaagccc ggcgccatcc tcatcaacgc ctcgcgcggc acggtggtgg agatcgagcc
       61 gctggcccag gcgctgcgcg ataaaaaact gctgggcgcg gcggtggatg tgttcccggt
      121 cgagcccaag agcaacaagg acgagttcca gtcgccgctg cgcgggctgg ataacgtcat
      181 catcacgccc catgtgggcg gctccacgca ggaggcgcag gccaacatcg gcctggaggt
      241 ggcggaaaag ctcgtccgct acagcgacaa cggcaccagc accacatcgg tcaactttcc
      301 cgaggtggcg ctgcccgcgc acccgggcaa gcaccgcatc ctgcacatcc acgccaaccg
      361 gcccggtgtc ctcggcgaaa tcaacagcaa gctctccaaa ttgaacgtca acatcctcgg
      421 tcaatacctg caaaccaacg accgtatcgg ctatgtagta accgatatgg acaaacgcac
      481 atcgtccaag gcattggaag cgctgaagaa agtcaagcat accattcgcg tccgcaacct
      541 gtattgatgg cgaacttgca accatttatc atccgtccgg cgcgtgcagc ggatgttccc
      601 gcgatgttcg cgctggtaaa ggaactggca ctcttcgaaa aagcgccaga acaggttacc
      661 aattccgaag cggacatgct ccgcgacgga tttggggaac aacccgtgtt ccgctctttg
      721 gttgctgaat gtgagggtca agtcgtgggg ctggccatct tcttcgtaaa gtactccacc
      781 tggaagggta aaggacttta cctcgacgac ctcatcgtta cagaaacgta ccgcggccgc
      841 ggcatcggac tggcactgct caaggcctac ctcgaagaag cccgacgctc cggtgcccgc
      901 caggtgcact ggcaagtcct cgactggaac caacccgcga tcgacctcta caagaaaatg
      961 ggcgcaagtt tggaagcgga atggttggat tgcaagatga atgttggtga atagtgaata
     1021 gtgaacagtg aacagtgaac agtaaacagt aaacagtaaa tagtaaatag taaatagtaa
     1081 atagcgaata gcgaatagcg aatagcgaaa aataaactgt aaacagtgaa tagtgaattt
     1141 tagctgccga agctttagcg taggcaggat ggaatagtga aaaatagtgc gaccgactgc
     1201 tttcgcaagg gttatggaaa aattatttgc aaattttatc
//
LOCUS       DAOTON010000877         1236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_89989_length_1236_cov_3.973751, whole genome shotgun sequence.
ACCESSION   DAOTON010000877 DAOTON010000000
VERSION     DAOTON010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89989_length_1236_cov_3.973751"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="P5292_13570"
     CDS             complement(<1..824)
                     /locus_tag="P5292_13570"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRS40203.1"
                     /translation="MSISTRIRTLLAACAILLAPATKAQLSFTNANNKIAGPMYSGCA
                     VTVTDVNFDGLDDILRMDQGHNVSLELQNRDGSFSNHYLTSIGSGSAWAMTCADVDHN
                     GWKDMIADGNSGIELVKIFESGGNITSTTTTLPNSGFFLQNATFCDMNNDGWIDLFCC
                     DDNAVSKLYVNDGSGNLFPSTIVNFAINPGINYNGDPADSGNYGSCWIDFDNDHDLDL
                     YVAHCRQSTSSPTDLRRINRLFVNDGNNNFTEAAGTYGIDIGWQTWTASFGDIDNDG"
     gene            complement(865..>1236)
                     /locus_tag="P5292_13575"
     CDS             complement(865..>1236)
                     /locus_tag="P5292_13575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40204.1"
                     /translation="MFTLNSAEIDSFRHMFQNADKTPFLMLRDSQWVNTTSIRPLNKP
                     LVDLYPTVSSGMPVTIKAINGNKILAVDCYSASGTRIQSVRPSSKTTVLEIQLDGSNG
                     YYFLRIRTEQGDTVRKVLLNR"
BASE COUNT          232 a          295 c          402 g          307 t
ORIGIN      
        1 ccatcgttgt caatgtcgcc gaagctcgcg gtccaggtct gccagccgat gtcgataccg
       61 taggtgccgg ccgcttcggt gaagttgttg ttgccatcgt tcacgaagag gcggttgatg
      121 cggcggaggt cggtcgggct ggacgtgctc tgacggcaat gcgcgacgta caagtcgagg
      181 tcgtggtcgt tgtcgaagtc gatccagcaa ctgccatagt tacccgaatc agccggatca
      241 ccgttgtagt taatgccggg gttgattgcg aaattgacga tcgtgctcgg gaacaggttg
      301 cccgacccgt cgttgacata gagtttgctg acggcattgt catcacagca gaacaggtcg
      361 atccagccgt cgttgttcat atcacagaac gtggcgttct ggaggaagaa tcccgaattc
      421 gggagcgtgg tggtggtgga agtgatgtta ccgcccgatt cgaagatctt gaccagttcg
      481 attccggagt ttccgtcggc gatcatgtct ttccagccat tatggtccac gtcggcgcag
      541 gtcatggccc aggcgctgcc ggagccgatg ctggtgaggt agtgattgct gaaactaccg
      601 tcacggttct gcagttccag gctaacgttg tgtccctggt ccatgcgtag gatgtcgtcg
      661 agaccgtcga agttcacgtc ggtcaccgtg accgcgcagc cgctgtacat cgggccggcg
      721 atcttgttgt tcgcgttggt aaagctcagt tgagctttcg tggccggagc aagcagaatg
      781 gcacaggcgg ccagtagcgt ccggattcgg gtagagatgc tcatgcgttt gaggtgatga
      841 ggttgttagg gttggttggt tggattatcg gttcaggagg accttgcgca cggtgtcgcc
      901 ttgttcggtc cttattctta ggaagtaata gccattggat ccgtccaatt ggatttccag
      961 tacagtggtt ttggacgaag gacgtaccga ctggatgcgc gttccggaag ccgagtagca
     1021 gtcgactgcc agaatcttgt ttccgttgat cgctttgatc gtaaccggca taccggagga
     1081 gaccgtgggg tacaggtcga ccaggggttt gttcaggggg cgtatcgatg tagtgttcac
     1141 ccactggctg tcgcgcagca tgaggaacgg ggtcttgtcg gcattctgga acatgtgccg
     1201 gaagctgtcg atctcggcac tgttgagcgt aaacat
//
LOCUS       DAOTON010000878         1236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_90007_length_1236_cov_2.779848, whole genome shotgun sequence.
ACCESSION   DAOTON010000878 DAOTON010000000
VERSION     DAOTON010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90007_length_1236_cov_2.779848"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="P5292_13580"
     CDS             complement(<1..213)
                     /locus_tag="P5292_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40205.1"
                     /translation="MNRKRYPMKAACSWLLALLLFSNLVQAQPANTYTFTTGTGVSLE
                     TLSAPVVLVGSGVDDTPASAASAIGFS"
     gene            complement(342..>1236)
                     /locus_tag="P5292_13585"
     CDS             complement(342..>1236)
                     /locus_tag="P5292_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HRS40206.1"
                     /translation="IVTDFRGCSSTSTPVSVSTALYPTIASLNPSSGCPGDSIDVAGT
                     NFSTVVSVDFFGVPAGFRIVNANLLRIEVPPGALSGSINLATSTGCSESSSMSFTVNT
                     CGGSLLDMTLLIQGYYDGAGGMTGTLYNSGISFDPTDCEFLHVCLMDAGTLTETDCFD
                     EVLKTSGSLQISIPGGYVGSSFYLKITGRNILETWTTAPVQIVSPFTVLDLTTAANTV
                     FGDNQVQVSTAPARWAIWSGDLNQDDLIEATDYSTMENDVQQFLFGYVPSDITGDGIV
                     EASDYALVENNSLLFLFSITP"
BASE COUNT          334 a          324 c          315 g          263 t
ORIGIN      
        1 tgaaaatccg atggccgaag cggcagatgc cggagtatca tctacaccgg aacccaccag
       61 caccaccgga gcgctgagcg tctccaaaga aacaccggta ccggttgtaa aggtgtacgt
      121 attcgcaggc tgtgcctgga ccaggttaga aaagagcagt agagcaagca gccagctaca
      181 ggctgccttc atcgggtagc gtttccggtt cattcgaggg ggcgtttctc ctaaatatcg
      241 ttgatttcag aagaaaaacc atacccaagt ccggttttta tgcaaacgcg acaatttact
      301 tggtaaacct ccggccgacc attacgacct ttcaaatcta atcagggggt tattgagaat
      361 aggaacaaca aactgttgtt ttctactagc gcataatccg aggcctcaac gattccatca
      421 ccggtaatgt cagatggcac atagccgaat aggaattgtt gcacatcgtt ttccatggta
      481 ctataatcag tcgcctcaat caagtcatcc tgattcaaat cgccactcca gattgcccaa
      541 cgggccggtg cagttgaaac ctgaacctgg ttgtcaccga agacggtgtt ggctgcagtt
      601 gtcagatcaa gtacagtgaa cggactgacg atctgaaccg gggcggtcgt ccaggtttcc
      661 agaatgtttc ggcccgtgat cttgaggtag aaggaagagc ccacataccc gccaggaatc
      721 gaaatctgca ggctgccgct cgttttcagg acttcatcga aacagtcggt ctccgtcagc
      781 gttccggcat ccatcaagca gacatgcagg aattcacaat cggtaggatc gaacgaaatt
      841 cccgaattgt acaaggtacc ggtcataccg cccgcgccgt cataataacc ctggatcagc
      901 aaggtcatgt ccaggagcga acctccgcag gtattcacag taaacgacat ggaagacgat
      961 tctgaacaac cggtgctggt agcaaggtta atggagccac tcagcgcgcc gggtggaact
     1021 tcgatccgaa ggaggtttgc attcacgatc ctgaagcctg ctggcacacc gaagaaatcc
     1081 accgagacga ctgtcgaaaa attagtgccc gccacatcga tggaatctcc cggacaaccc
     1141 gaactcggat tcagcgaggc gatcgtcgga tacaaggccg tagagacgct gaccggtgtt
     1201 gaggttgacg aacaccctct gaagtcagta actatt
//
LOCUS       DAOTON010000879         1235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_90087_length_1235_cov_2.990678, whole genome shotgun sequence.
ACCESSION   DAOTON010000879 DAOTON010000000
VERSION     DAOTON010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90087_length_1235_cov_2.990678"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1235)
                     /locus_tag="P5292_13590"
     CDS             complement(<1..>1235)
                     /locus_tag="P5292_13590"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS40207.1"
                     /translation="YAVQVTDANGCTRTETVTIAEPAALQSASNAAAVRCNGGSDGSV
                     SVSVQGGTSPYTYQWSTGASSSSVDQLAAGNYSVTVTDDNGCTITGAVAVTEPPALLL
                     STQGDAWLCIGQSTQIQASAAGGTPGYAFLWSNGAQTANQTVQPAATSTYAVTLTDAN
                     GCIAGPQSVVLQLYPALTAIVSNADTLCPGDPATLTAFASGSNGGPYSYNWNTGASGP
                     SITVSPATTTLYTVTVSDQCGSTPVQLQVPVNVVAAPSSNFLPNPASGCVPLTVQYVN
                     SGNLPAGTSYLWDFGDGATSTDRNPAHTFAQPGAYTVRHFVITPLGCASQTLVPAAVN
                     VHPNPIADFTAQPEEVSIFRPRVEMINNSVRSVRWNWDFGDGLGNSAEREPVYTYRDT
                     GNYTIRLVSRTEHGCPDTT"
BASE COUNT          259 a          360 c          366 g          250 t
ORIGIN      
        1 aggtagtatc ggggcaaccg tgttccgttc gactgaccag tcggatcgta tagttgccgg
       61 tatcgcggta cgtgtaaacc ggctcgcgct ccgccgaatt gccgaggccg tcgccgaaat
      121 cccaattcca acgcaccgag cggacactgt tgttgatcat ttccacccgt ggacggaaga
      181 tcgacacctc ttccggctgc gcggtaaagt cggcaatcgg attcggatga acattgaccg
      241 ctgcaggaac aagcgtttgc gatgcacaac ccaatggcgt gatcacgaag tgccgcactg
      301 tataggcgcc gggctgtgcg aacgtatggg ccgggttcct gtcagtgctg gtagcgccgt
      361 caccgaagtc ccagagataa ctggtgcccg ccggtaagtt accgctgttg acatattgca
      421 ccgtcagcgg aacacaacca ctcgccggat tcggcaggaa gttcgaggaa ggcgccgcga
      481 ccacgttcac cggcacctgc aactgcaccg gcgtgctgcc gcactggtcg ctgaccgtca
      541 ccgtgtagag cgtagtcgtt gcaggactga cggtgatcga aggtcctgaa gcaccggtat
      601 tccaattgta tgaatacggc ccgccgttac tgccgctggc aaaggccgtt aacgttgccg
      661 gatcgcccgg acaaagtgta tctgcattcg atacgatcgc cgtcagtgca ggatacaatt
      721 gcaacacgac ggattgcgga ccggcaatac atccattcgc gtcggtaagt gtaaccgcat
      781 acgtactggt agctgcaggc tgtacggtct gattggccgt ctgcgcgcca ttgctccaga
      841 ggaatgcata gccgggagtg ccgccggcgg cgcttgcctg gatctgcgtt gactggccga
      901 tacacaacca ggcgtcacct tgcgttgaaa gcagcaaagc aggtggctcc gtgaccgcga
      961 ccgcgccggt aatggtacag ccgttgtcgt cggtaaccgt tacgctgtag ttgccggccg
     1021 cgagttgatc cacgctggaa gaacttgcac cggtactcca ttgataagta taaggtgagg
     1081 tgccgccttg aacagaaacg ctgaccgatc cgtcgctacc tccattacag cgcactgccg
     1141 ccgcattcga tgcagactgc aatgccgcag gctcagcgat cgtcacggtt tccgtacggg
     1201 tacaaccgtt cgcatcggtg acctggacgg catat
//
LOCUS       DAOTON010000880         1233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_90364_length_1233_cov_2.046689, whole genome shotgun sequence.
ACCESSION   DAOTON010000880 DAOTON010000000
VERSION     DAOTON010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90364_length_1233_cov_2.046689"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1233
                     /locus_tag="P5292_13595"
     CDS             <1..>1233
                     /locus_tag="P5292_13595"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRS40208.1"
                     /translation="PAQCTDWRFGYSICCRNCAITTLAFAGGTCAGSPALYVEATLNN
                     VAAPTNSSPVFSNIPVSFLCIGQVFHYNHGAYNTDGDSITYAFITPRSAANTNVTFNA
                     GYTTTSPLTSSPAMSIDASGDITVAPTQTEVGVLAIIVREYRNGVLVGSVIRDMQIWT
                     VPCSNLLPTATGINGTNNFQIVACPGRPLNFTVNSADANASQTVSMNWNYAIPGATFT
                     PSAASRPVGTFSWTPTIADARPQPYSFTVTVQDNNCPSAGFQTYSYDVLVPPLSATAT
                     STNSRCSSPVNGTATALPVGTGPYQYAWSPGGATTATASNLGPGTYTATITDTYGCTT
                     TASTTLTAPPPVAISIAAQANVSCRNGNDGAAAITASGGTAPYTYSWSPNGGTASSAS
                     GLSVGNYAVTVTDANGCTR"
BASE COUNT          265 a          399 c          301 g          268 t
ORIGIN      
        1 accggcccaa tgcaccgact ggcggttcgg ctattccatc tgctgtcgta attgcgcaat
       61 caccacactg gcattcgcag gaggaacctg cgcgggatca cccgccctct atgtcgaagc
      121 tacgttgaac aacgtagctg cgccgaccaa ctcttctccg gtattctcca atattccggt
      181 ttctttcctg tgtatcggac aagtgtttca ctacaatcac ggtgcctaca ataccgacgg
      241 cgattcgatc acgtatgctt tcattacacc gcgttcagcg gccaatacga acgtgacgtt
      301 taatgcgggt tatacgacca ccagtccgct cacttcttcc ccggccatgt ccatcgacgc
      361 aagcggcgac atcaccgtcg ccccgacgca aacggaagtc ggcgtactgg ccatcattgt
      421 ccgcgaatac cggaacggcg tgttggtcgg cagtgtgatc cgcgatatgc agatctggac
      481 cgtcccctgc agtaacctgt tgccaacggc caccggcatc aacggtacga acaacttcca
      541 gattgtcgcc tgcccgggcc ggcctttgaa ctttacggtc aactcagccg atgcgaatgc
      601 ttcgcaaacc gtgtcgatga actggaacta tgcgattccc ggagcgactt tcacgccttc
      661 agcagcatca agaccggtgg gcacattctc ctggacgccg accatcgccg atgcacgacc
      721 gcaaccgtac agctttaccg taacggtgca ggacaacaac tgtccgagtg cgggattcca
      781 gacgtattct tatgatgtgc tggtcccccc tttgagtgcg accgccacgt ccaccaattc
      841 ccgttgttca agtcctgtga acggaactgc aacggcattg cccgtcggaa caggccccta
      901 tcaatacgcc tggtcgcctg gcggcgctac tactgcaact gccagcaacc tcggtcccgg
      961 aacctatact gccaccatca cggataccta tggctgtact accacagcat cgactactct
     1021 caccgctcca cctcctgttg ccatcagtat agcagcacag gcaaatgtct cttgcagaaa
     1081 cggcaacgat ggcgcggcgg ccatcaccgc ttcgggtgga accgcacctt atacctatag
     1141 ttggtcaccc aacggcggga cagcatcttc ggcttccgga ctttctgtcg gtaactacgc
     1201 agttacggtt actgatgcga acggctgtac gcg
//
LOCUS       DAOTON010000881         1231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_90569_length_1231_cov_2.477041, whole genome shotgun sequence.
ACCESSION   DAOTON010000881 DAOTON010000000
VERSION     DAOTON010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90569_length_1231_cov_2.477041"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="P5292_13600"
     CDS             complement(<1..1150)
                     /locus_tag="P5292_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HRS40209.1"
                     /translation="MNVRYLCLLLLGFLLLTSGSNATCPGGNSEVIVQIIPDSYPNEI
                     SWEIYDISGTLLGSGGPVGDTLCVPSNTCTQFIMHDSYGDGIYAPGGYWLYIDGALAT
                     SGNAFGSQATFQFNCPPGAYCTGPLPINTGTYTTLFDDSWYSFTPGQTGTYNLSTCTG
                     NTCNTKIWIYDNCPGLPYPEDAPGSYAFNDDFCGTQASLNVMLVAGTPYYFRIGDNND
                     DCNGPIDFSFSYVGPVQGCTDQSACNYNPLAVIDDGSCIYFPDPGCAGPDLRFDSVSF
                     VSSLSMAAHNAATCDVAEGCVTGYGQRYVLRFTSKIDNIGPQDYYIGTPQTQPNMFNL
                     NNCHGHAHYEGYGDYRLFDLNGNIVPAGHKNGFCVMDLCGMGQYSCGNM"
BASE COUNT          289 a          310 c          363 g          269 t
ORIGIN      
        1 ccatgtttcc gcagctatac tgtcccatgc cgcagaggtc catcacacag aatccgttct
       61 tgtgaccggc aggcacgatg ttaccgttca ggtcgaacag gcgataatca ccgtagcctt
      121 cgtagtgggc atgaccatgg cagttgttca ggttgaacat gttcggctgg gtctgcggcg
      181 tgccgatgta ataatcctgc gggccgatgt tgtcgatctt ggaagtaaag cggagtacat
      241 aacgctggcc gtaacccgtc acacaacctt ccgctacgtc gcaagtcgcg gcattgtgag
      301 cggccattga cagactgctc acgaacgaaa cggaatcgaa ccgaagatcg ggtcctgcac
      361 agcccggatc ggggaagtag atacaactgc cgtcatcgat cacggctaac ggattgtagt
      421 tacacgcgct ctgatcggtg caaccctgaa ccggacccac ataactgaag gagaaatcga
      481 tcggaccatt gcagtcgtcg ttgttgtcgc cgattcggaa gtaatagggt gttccggcaa
      541 cgagcatcac gttcaggctt gcctgtgtgc cgcagaaatc gtcgttgaaa gcataggatc
      601 ccggcgcgtc ttccggatag ggcaagccgg ggcagttgtc atagatccag atcttggtgt
      661 tgcaggtgtt acccgtacag gtggatagat tgtaggtgcc ggtctgacca ggtgtaaagg
      721 aataccagga atcgtcaaac agcgtggtgt acgtgccggt attgatcggc agcggcccgg
      781 tgcaataggc tccgggcgga cagttgaact ggaatgtggc ctggctgccg aaggcgttgc
      841 cggaagtggc tagcgctccg tcgatataca accagtaacc gcccggtgca tagatgccgt
      901 cgccatagct gtcgtgcatg atgaactggg tgcaggtatt cgacggtaca caaagcgtgt
      961 cgccaaccgg accacccgaa ccgagtaagg ttccggagat gtcatagatc tcccaactga
     1021 tttcgttggg ataactatcc ggaatgatct ggacgatcac ttcactgttg ccacccgggc
     1081 aggtggcgtt tgatccggaa gtcaggagta ggaagcccag caggagcagg cagaggtaac
     1141 gtacgttcat gaaagatgga ttgattcggc ctaatttacg ggaatattgt catgccgggg
     1201 cttgtaaatt cacataccca ttcacccatg a
//
LOCUS       DAOTON010000882         1229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_90850_length_1229_cov_1.916525, whole genome shotgun sequence.
ACCESSION   DAOTON010000882 DAOTON010000000
VERSION     DAOTON010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90850_length_1229_cov_1.916525"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="P5292_13605"
     CDS             <1..767
                     /locus_tag="P5292_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS40210.1"
                     /translation="SPLSVTFNGTSTGAAFYSWDFGDGTVLSEVELDTVSHTYTGNGS
                     YVPQFYLGFQLPVSQSFCYVPVPIFDTVKVTSLIGVDILEDTIVVTDGEQDTLHVFVT
                     DPNNNPPYTYNWTPASQVTQDLLDDETFYATTTGNTQYYYVSVPFGVSGCSAIDSVLV
                     VYRVCEGELKIPNVFTPDDDGKNDTYYVKDLCNYDGFEFRIYNRWGRIIYESSDPDFQ
                     WDGKTTGGELANEGVYYYVMKTKTKELKGWIQLIRE"
     gene            complement(773..>1229)
                     /locus_tag="P5292_13610"
     CDS             complement(773..>1229)
                     /locus_tag="P5292_13610"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRS40211.1"
                     /translation="SQVAGRSGRRSKQGKVIIQTRNPGHWVIRAVMNHDYASFYHQDM
                     EERRHFGYPPHCRLVSFTIRHREEALTHDAATRFCTLLRNKFGSRVHGPHIPVVHRVR
                     NQYIRHILLKIEREQKTAGVRETIRSCMREFYQERIYHPVQLFADVDPV"
BASE COUNT          285 a          345 c          319 g          280 t
ORIGIN      
        1 gcagtcccct ttccgtcacg ttcaacggaa caagtaccgg cgctgcgttc tactcttggg
       61 atttcggtga cggaacggtt ctcagtgaag tggaactcga caccgtttcg catacctaca
      121 ccggcaatgg tagctacgtt ccccaattct atctcggatt ccagctaccc gtcagccagt
      181 cgttctgtta tgtgccagta ccgatctttg acaccgtaaa ggtcacgtcc ctcatcggtg
      241 tcgacattct ggaagacacg atcgtagtca cggacggaga acaggacacg ctccacgtct
      301 tcgtcaccga tcccaataac aacccgccct acacctacaa ctggacccct gccagtcagg
      361 tgacccagga cctgctggac gacgagacgt tttacgccac gaccactggc aatacccagt
      421 actactatgt cagtgttccc tttggagttt ccggctgctc tgccatcgat tccgtgctcg
      481 tggtgtaccg tgtctgcgag ggcgaattga agatcccgaa cgtgttcacg ccggatgatg
      541 acggcaagaa cgatacctac tacgtcaagg acctttgcaa ttacgacggt ttcgagttcc
      601 gtatctacaa tcgctggggt cgcatcattt acgaatcttc ggacccggat tttcaatggg
      661 atggaaagac gaccggaggc gaactggcca acgaaggcgt ctactattac gtgatgaaga
      721 cgaaaacaaa ggagttgaaa ggctggatcc agttgatccg ggagtgatcg tcttatacgg
      781 gatcaacgtc cgcaaaaagc tggaccggat ggtagatccg ctcctggtaa aattcacgca
      841 tgcaggatcg aatggtctca cgaacgcccg cggttttctg ctcgcgttcg atcttgagca
      901 aaatgtggcg gatgtactga ttccgtacac ggtgaacgac cgggatatgt ggaccgtgga
      961 cccgcgaccc gaatttgttt cgtaacaaag tacagaaccg cgtagccgcg tcatgcgtca
     1021 gggcttcctc ccgatgacgg atcgtgaagg acaccaatcg gcaatgcggc ggataaccaa
     1081 agtggcgccg ctcttccatg tcctggtggt aaaaggaagc atagtcgtga ttcatcacgg
     1141 cccggatgac ccaatgcccc ggattacgcg tctggataat gacctttcct tgtttgcttc
     1201 tcctgcccga ccgacctgca acctgtgac
//
LOCUS       DAOTON010000883         1226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_91124_length_1226_cov_2.874466, whole genome shotgun sequence.
ACCESSION   DAOTON010000883 DAOTON010000000
VERSION     DAOTON010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91124_length_1226_cov_2.874466"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /gene="moaCB"
                     /locus_tag="P5292_13615"
     CDS             complement(<1..883)
                     /gene="moaCB"
                     /locus_tag="P5292_13615"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HRS40212.1"
                     /translation="MIDITHKSNTLRYARAQAVVRVSDPATLQAIREGKVPKGDVPAT
                     AKAAALLAIKRTSDVIPDCHPLPVEFADVRFEYSELELTIIVEVKTIYKTGVEVEAMH
                     GASVAALTAYDMLKPLDKGIEIAQVRLLEKRGGKTEFKEAYRKSLTSAVVVCSDSVSA
                     GRKEDEAGKAVLRRLEQLEIPVTGYHIIPDEVASIQALVKRLATEEHDLVVLTGGTGL
                     SPRDVTPEALQPILDRTIPGIAEAMRTHGQERMPYAMLSRSLAGLIGRTLVLALPGST
                     RGAEESMDALFPHVLHIF"
     gene            complement(891..>1226)
                     /locus_tag="P5292_13620"
     CDS             complement(891..>1226)
                     /locus_tag="P5292_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRS40213.1"
                     /translation="EIFLGQIRADKKGTETVEAIEFSAYREMADTAYQEFREQLFARH
                     SITCMHVYHSLGRVRAGELNLFVFVSAERRVAAMDACRELVEWIKKELPVWGKEVLSS
                     AAGEWKVNT"
BASE COUNT          267 a          396 c          307 g          256 t
ORIGIN      
        1 ggaagatatg caacacatgc gggaacaaag catccatact ttcctccgct ccccgcgttg
       61 atccgggcaa tgccaacacc agcgtacggc cgatcaaacc ggccagactg cgcgacaaca
      121 tcgcgtacgg catgcgctcc tgaccgtgcg ttcgcatcgc ctcggcgatt ccggggatcg
      181 tccggtcgag gatcggctgc aaggcttccg gggtgacgtc gcgcggcgac aagccggtgc
      241 cgcccgtgag caccacgagg tcatgctcct cagtggccaa acgcttcaca agcgcttgga
      301 tcgatgccac ttcatcgggt atgatatgat agccagtcac cggaatctcg agctgctcca
      361 gccgacgaag tacagcctta ccggcttcgt cctctttccg gcccgcggaa acggaatcgg
      421 aacaaacaac gacggccgaa gtcaacgact tccggtaggc ttccttgaac tcggtcttac
      481 cgccccgctt ctcaagcaac ctgacctgtg cgatctcgat tcccttatcc agcggcttca
      541 gcatatcgta ggcagtcagc gcggcaaccg atgctccatg catcgcttcc acctccacgc
      601 cggtcttgta aatcgtcttc acttccacaa tgatcgtgag ttccaattcg gaatattcaa
      661 accggacatc cgcgaattca accggtaacg gatgacaatc gggaatgaca tcacttgtcc
      721 ttttaatcgc caacaaggct gccgccttgg cggttgccgg cacgtcgcct ttgggcactt
      781 tcccttcgcg aatggcctgt agcgtcgcgg ggtcgctcac gcgcaccacg gcctgtgccc
      841 gggcatagcg tagcgtatta ctcttatggg tgatatcgat catgtacgtt tcaagtattg
      901 actttccact ccccggctgc agaagaaagg acttccttcc cccagacggg cagctctttc
      961 ttgatccatt cgaccagttc gcggcaggca tccatagccg caacacgacg ttcagcggaa
     1021 acgaaaacga agaggttcaa ctctcctgca cgcacccgcc caagcgaatg gtaaacatgc
     1081 atacaggtga tggaatgacg cgcgaacaac tgctcccgaa actcctgata agccgtgtcg
     1141 gccatctcac gataagcgct gaactcgatc gcctccaccg tttccgttcc cttcttgtcc
     1201 gcacggatct gcccaaggaa gatctc
//
LOCUS       DAOTON010000884         1226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_91167_length_1226_cov_2.082835, whole genome shotgun sequence.
ACCESSION   DAOTON010000884 DAOTON010000000
VERSION     DAOTON010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91167_length_1226_cov_2.082835"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..573)
                     /gene="ybeY"
                     /locus_tag="P5292_13625"
     CDS             complement(151..573)
                     /gene="ybeY"
                     /locus_tag="P5292_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRS40214.1"
                     /translation="MSATILFFKEDVRFVFRGADDLRKWIQKAAKAEGHRVAEINYIF
                     CSDRYLRKMNKQYLQHDYNTDIITFDNSPGPNIIGGDIFISIDRVKINAKTYGSTFTD
                     ELHRVMIHGILHLCGYRDKTEAEKVKMRGREEYWLAKR"
     gene            611..>1226
                     /locus_tag="P5292_13630"
     CDS             611..>1226
                     /locus_tag="P5292_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40215.1"
                     /translation="MLSRIKVTLLGSLLSWSCLAHAQDDMGWWNERHQWDGVSPWVNY
                     LTLSPKFMGPNALPVTEIRSGMLRNNLEVETRFGGYGSRGDNTFDTYGRVNVPLCNGR
                     VALEGWVTALEYFETDTTTRDLRAARTRSGKGTAGGDIHFSTQVQLVKDKAHWPDLLL
                     ELTMRTASGTRLRDARYTDASGYWFTLSAGKEKKTGGKLITAFRP"
BASE COUNT          250 a          321 c          360 g          295 t
ORIGIN      
        1 ggtttccagt ttccggttac gagtttcggg tttgttgaag gttgaaagtt tatttcagcc
       61 tcatttcctc gtctctttcg cctcgtccct ctttttgact tgaaacccga aacccgaaac
      121 tcgaaaccgg aaacaatgaa cggattgact tcaccttttc gctaaccaat attcctcccg
      181 ccccctcatc ttcacttttt ctgcttcggt cttgtcgcgg tagccgcaga ggtggaggat
      241 gccgtggatc atgacgcggt ggagctcgtc ggtgaaggtg ctgccgtagg tcttggcgtt
      301 gattttgacg cgatcgatcg agatgaaaat gtcgccgccg atgatgtttg gtccgggcga
      361 attgtcgaag gtgatgatgt cggtattgta atcgtgttgc aggtattgct tgttcatctt
      421 gcgcaggtag cgatcgctgc agaaaatgta attgatctcc gcgacccgat ggccttccgc
      481 tttagcggcc ttctggatcc acttgcgcag gtcgtcggcg ccccggaaga cgaagcgcac
      541 atcttctttg aagaacagga tggtggctga catacatcga aaggtacgaa tcgggtcgta
      601 tctttcgact atgctaagcc ggataaaagt cacgttgctg ggaagcctgt tgagctggtc
      661 ctgtcttgca catgcgcagg acgatatggg ctggtggaac gagcgtcatc agtgggatgg
      721 tgtttccccc tgggtcaact acctgacatt gtcgccgaag tttatgggcc cgaacgcgct
      781 gccggtgacc gagatccgca gcggaatgtt gcgcaataac ctggaagtgg aaacgcgttt
      841 cggcgggtac ggcagtcggg gcgacaatac ctttgatact tatggccgcg tgaacgtacc
      901 cttgtgcaac gggcgtgtcg cgctggaagg ctgggtcacc gcgctggaat atttcgagac
      961 cgatacgacc acccgcgacc tgcgcgcggc gcgcacgcga tccggaaaag gaaccgccgg
     1021 tggtgatatc cacttcagca cgcaggtgca actggtgaaa gacaaagccc actggcccga
     1081 tctgctgttg gaactgacga tgcgaaccgc ttccggcacc cgactgcgcg acgcgcgcta
     1141 taccgatgca tccggctatt ggttcacgct ctcggcggga aaggaaaaaa agaccggcgg
     1201 taagttgatc accgcgttcc gtccgt
//
LOCUS       DAOTON010000885         1225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_91257_length_1225_cov_2.854701, whole genome shotgun sequence.
ACCESSION   DAOTON010000885 DAOTON010000000
VERSION     DAOTON010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91257_length_1225_cov_2.854701"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="P5292_13635"
     CDS             complement(<1..790)
                     /locus_tag="P5292_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRS40216.1"
                     /translation="MANTSTVEKFVKYKVKDISLAEWGRKEIKLAEAEMPGLMALRAE
                     YGAQKPLKGARIAGCLHMTIQTAVLIETLVELGAEVTWSSCNIFSTQDHAAAAIAAAG
                     ISVYAWKGMNAEEFDWCIEQTLFFGEDRKPLNMILDDGGDLTNMVFDKYPELITGIKG
                     LSEETTTGVLRLHERMKNGTLPIPAINVNDSVTKSKFDNKYGCRESLVDAIRRATDLM
                     MAGKVAVVAGFGDVGKGSAESLRDSKVRVIVTEIDPICALQAAME"
     gene            928..>1225
                     /locus_tag="P5292_13640"
     CDS             928..>1225
                     /locus_tag="P5292_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore biosynthesis protein"
                     /protein_id="HRS40217.1"
                     /translation="MPIVFRYGNDDAALSGAVWQCTESTEALGESLSLSPESRALLAS
                     FRSERRRREWLTVRRLLNELHPDQPAPEILYSGNGRPSLSDGSHISISHSEHYVT"
BASE COUNT          238 a          357 c          341 g          289 t
ORIGIN      
        1 cttccatggc cgcctgcagg gcgcagatcg ggtcgatctc ggtcacgatc acgcggactt
       61 tggagtcgcg cagcgattcg gccgaaccct tgccaacgtc gccgaagccg gcaacgacgg
      121 caactttacc ggccatcatc agatcggtcg cgcgacgtat cgcgtcgacg agcgactcgc
      181 ggcagccgta tttgttgtcg aacttcgact tggtcacgga atcgttcacg ttgatcgcgg
      241 ggatcggcaa ggtgccgttc ttcatgcgct cgtggagacg gagaacaccg gtggtggtct
      301 cttccgacaa gcccttgatg ccggtgatca gctcggggta tttgtcgaat accatgttgg
      361 tcaggtcacc tccgtcgtcg aggatcatgt tgagcggctt gcggtcttct ccgaagaaca
      421 gcgtctgctc gatgcaccag tcgaattctt ccgcgttcat gcctttccag gcgtagaccg
      481 aaataccggc tgcggcaatt gctgcggcag cgtggtcctg ggtagagaaa atgttgcagg
      541 acgaccaggt cacttccgcg ccgagttcaa ccagcgtttc gatgagcaca gccgtttgaa
      601 tcgtcatgtg caggcatccg gcgatccggg cacccttgag cggcttctga gcgccgtact
      661 cggcacgcaa tgccatgagg cccggcattt cagcttcggc cagtttgatc tccttgcggc
      721 cccattcggc cagggaaatg tcctttacct tgtatttcac gaatttctcg actgtggagg
      781 tgtttgccat atggttttga agtgacttag tttgctgaat ttcaggctgc aaagatacga
      841 ttttaccggc ttattcccca accgaaaatc agggattttc tgctaaaacc gggctcagga
      901 agtatcttgc aggacaaaca gcgaggaatg ccaattgttt tccgatacgg caacgatgat
      961 gctgcgcttt ccggtgctgt ctggcagtgt accgaatcga cagaggccct tggcgaatcc
     1021 ctttcgttga gtcccgagtc tcgtgcgctc ctggcatcct tccgcagcga aagacgccgg
     1081 cgggaatggc tgaccgttcg ccggctgctc aacgaactgc atcccgatca gcccgccccc
     1141 gagatcctgt attccggaaa cggacgccct tcgctttccg atggcagcca catttccatt
     1201 tcacactccg aacactatgt aaccc
//
LOCUS       DAOTON010000886         1224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_91334_length_1224_cov_4.240376, whole genome shotgun sequence.
ACCESSION   DAOTON010000886 DAOTON010000000
VERSION     DAOTON010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91334_length_1224_cov_4.240376"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="P5292_13645"
     CDS             complement(<1..531)
                     /locus_tag="P5292_13645"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRS40218.1"
                     /translation="MKLKRLLILLFFPVFATGQTGLSLKEAMDRALQNNLEVRLARNN
                     AEAAEWNNHPGVAGALPTVNLSGGDVQQSTNIYQKLANGTIIERDGAAGNTMNGALAV
                     SYTLFNGYRISATRERLARLQEAGDAQLLSQIQSTLAAVIGRYYDVQRQEQVGQALTR
                     SRDFVAERVKVLETRAR"
     gene            complement(528..>1224)
                     /locus_tag="P5292_13650"
     CDS             complement(528..>1224)
                     /locus_tag="P5292_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRS40219.1"
                     /translation="LYHFNRFKSATVSASLAEGKTIGDGVEVMRGIADTLLDESFQTS
                     LSGPSRDFAESSSNILFAFLLALLLIYLILAAQFESFRDPLTIMITVPLALAGALLSL
                     WIFGQTLNIFSQIGMIMLIGLVTKNGILIVEFANQKRASGLPLREAVLEAASHRLRPI
                     LMTSLATALGALPIALSLGAAATSRIPLGIVIVGGLLFSLVLTLFVIPSLYLVITGKR
                     HDRVTAGDESESA"
BASE COUNT          265 a          349 c          367 g          243 t
ORIGIN      
        1 ccgcgcccgt gtttccaaca ccttgacacg ctccgcaacg aaatcccggc tgcgtgtaag
       61 cgcctgtccc acctgctcct gccgctgaac gtcgtagtag cgaccgatca ccgcggccaa
      121 ggtgctttgt atttgcgaca acaactgggc gtcacccgct tcttgcagcc gggcgagtcg
      181 ctcgcgcgtg gcgctgatgc gataaccgtt gaacagggta taggaaacgg cgagggcgcc
      241 gttcatcgta ttgccggcag cgccgtcgcg ctcgatgatc gtaccgttgg cgagtttttg
      301 atagatgttg gtactctgct gcacatcgcc tcccgacagg ttcacggtcg gcagtgcgcc
      361 tgccacaccc ggatggttgt tccattcggc cgcctccgca ttgttcctgg ccagacggac
      421 ttcgaggttg ttctgcaagg cacgatccat cgcttccttc agtgaaaggc cggtttgtcc
      481 cgttgcaaac accgggaaga aaagcaggat caggagtcgt ttcaacttca tgcggattcg
      541 gattcgtcgc cggccgttac ccgatcatgc cgcttgccgg tgatgacgag gtaaagcgac
      601 ggaattacga agagggtcag tacaagcgag aacagcaggc cgccgacgat gacaatcccg
      661 agtgggatgc ggctggtggc ggcggcgccc aggctgagcg caatcggcag agcaccgagc
      721 gccgtggcca ggctggtcat gagaatcggg cgaaggcgat gtgaggctgc ttctaacacc
      781 gcttcccgta aaggcaaacc cgacgctcgc ttttggttcg cgaattcaac gatgaggatg
      841 ccgttcttcg tcaccaggcc gatgagcatg atcatgccga tctgagagaa gatattaagc
      901 gtctgaccga agatccacaa gctcaacaag gctccggcaa gcgccagcgg tacggtgatc
      961 atgatcgtca gcggatcacg gaaactctcg aattgcgccg cgaggatcag gtagatcagc
     1021 aagagggcca gcagaaacgc gaagaggatg ttcgaagaac tttccgcgaa gtcgcgcgaa
     1081 ggaccactga gtgatgtctg aaacgattcg tccagcaagg tatccgcgat cccgcgcatc
     1141 acttcaacgc cgtctccgat cgtcttccct tccgcgaggg atgccgaaac ggtggctgat
     1201 ttgaatcgat tgaaatgata cagg
//
LOCUS       DAOTON010000887         1224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_91338_length_1224_cov_4.005988, whole genome shotgun sequence.
ACCESSION   DAOTON010000887 DAOTON010000000
VERSION     DAOTON010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91338_length_1224_cov_4.005988"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="P5292_13655"
     CDS             <1..911
                     /locus_tag="P5292_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40220.1"
                     /translation="SVLLSLLLLAGSATACEMCNCYLGINPNYNQHMIGVNFRHRVYQ
                     GVHVHQASDGHNHVHPGEVTPTIRENFQTTDLNLRLYPLPELQLFVNLPYSYNYIETN
                     GMVTDVIKGFGDAFILGQYQVLNTMPDSDNVRHRVFVGGGVKAPSGVYDKPSDDGEIE
                     AHYQPGTGTWDLLLAGSYLLKWRQWGASSDVTFRTGRKNDLGYHMGDRFNVNTSIFHQ
                     FVVKDWTMLPSLGAFFEQAGEDKQDELTVSNTGGWGVLGSAGFDLYYGRTSLHLAGQL
                     PLAQDLVGTQTENKFRLIAGLQFAFR"
     gene            1020..>1224
                     /locus_tag="P5292_13660"
     CDS             1020..>1224
                     /locus_tag="P5292_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40221.1"
                     /translation="MSIRYSLNNRVRLLLYLFIFSFSVPTSVWGAEAPWKETTPDKIP
                     NRELRTDLPVSYRSFELDLSVLQS"
BASE COUNT          290 a          294 c          315 g          325 t
ORIGIN      
        1 tttccgtcct gctgtccctc ttattgttag ccggatcagc aacagcctgt gaaatgtgca
       61 actgctatct cggaatcaat ccgaattata atcagcacat gatcggcgtg aatttccgtc
      121 accgcgtgta tcaaggagtg catgtacatc aggccagtga cggtcacaat catgtgcatc
      181 cgggggaagt gacgccgacg atccgggaga attttcaaac caccgatctg aacctgcggc
      241 tctatccttt gccggaattg caacttttcg tcaatcttcc ctacagctac aattacatcg
      301 agacgaacgg aatggtcacc gatgtgatca aaggatttgg cgatgctttc atcttgggcc
      361 agtatcaggt gctgaacacg atgcccgatt cggacaatgt acgtcatcgg gtttttgttg
      421 gaggaggtgt aaaagctcca agcggcgtgt acgacaagcc ttccgatgac ggagaaatcg
      481 aagcgcacta ccagcccgga actggcacat gggatttgtt attggcgggt agttacctgc
      541 tgaaatggcg gcaatggggc gctagtagcg atgtcacttt ccgcaccggg agaaagaacg
      601 atctgggtta tcacatgggc gatcggttta atgtcaatac ttccatattt catcagtttg
      661 ttgttaagga ttggacgatg ctacccagcc tgggagcgtt tttcgagcag gcaggtgagg
      721 ataagcagga tgaactgacg gtcagcaata ctggcggttg gggcgtgttg gggagtgccg
      781 gtttcgatct ctattatggt cgtacctctc tccacctggc cggacaactg ccattggccc
      841 aggatcttgt cggtacccaa accgagaata aattccgcct gattgccggt ctccagtttg
      901 cttttcgctg agttatttaa ctggaattca atgcattaaa atttcattaa agccgctgat
      961 ttcgggggta tatttctgtt ttacaattaa cccggactgc gaaccggtca aagccaccta
     1021 tgagcattcg ttactccctc aacaacaggg taaggctact gctttaccta ttcattttct
     1081 ctttcagtgt gccgacgtcg gtttggggcg ctgaagcacc ttggaaggag acgacacccg
     1141 ataagatacc gaatcgcgaa ctgcgaacgg atctgccagt ttcttatcga agctttgagc
     1201 tcgacttatc cgtgttgcaa tcca
//
LOCUS       DAOTON010000888         1224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_91396_length_1224_cov_2.076133, whole genome shotgun sequence.
ACCESSION   DAOTON010000888 DAOTON010000000
VERSION     DAOTON010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91396_length_1224_cov_2.076133"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="P5292_13665"
     CDS             <1..1191
                     /locus_tag="P5292_13665"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRS40222.1"
                     /translation="GSISLGYNIARDSMFMLIDNQRRNGQGATQNTLESAYTGQLAVG
                     YTDRTDAQTNFGFNTEHTFPQTYFSSAEPMKSDLHHLFPCDDLSNNQRGNNPFAEVTS
                     PTWSDGGSTSDGFRFEPRDAHKGRAARAMFCFTLRYQNYNGFLTSQENVLRNWHAAFP
                     PDAIDRARNEAIFSLQRNRNPFIDYPQFIDRITSISTLSTAPVVRSLDPMQDTIIYGS
                     VLPAATTVYTFVVVNNGNTAVNFSNFNLTPTPVLSFAGTGVDTTIGPGDALALQIACT
                     PSTMSAVNGFLTFNTDVPGSTSFSIPIYANDPVFSTIDETNRKSLLVFPNPASDLVWL
                     TTESITDTPVFVYDLAGRALGQLPTQQLGHRLQLSLAGLAPGCYLIGLHTIAGPVFTR
                     VVKE"
BASE COUNT          264 a          420 c          286 g          254 t
ORIGIN      
        1 ggttcgatct ccctgggtta caacatcgcc cgggattcga tgttcatgct gatcgacaac
       61 caacgccgca acggacaagg cgcgacgcag aatacactcg agagcgctta taccggacaa
      121 ctcgccgttg gctacaccga ccgtaccgat gcgcagacga acttcggttt caataccgaa
      181 catacgttcc cgcagactta tttcagcagc gccgagccga tgaaatcgga tctgcatcac
      241 ctgtttccct gcgacgacct gtccaacaat cagcgtggta acaatccctt cgcggaagta
      301 acctcaccca cctggtccga tggcggttcc acgtccgatg gctttcgctt cgaaccccgc
      361 gacgcgcaca agggacgcgc cgcacgcgcg atgttctgct tcacgctgcg ttaccagaat
      421 tacaacggct tcctcaccag ccaggagaat gtcctgcgca actggcacgc ggcctttccg
      481 cccgatgcga tcgaccgcgc acgcaacgaa gcgatcttct ccctgcagcg caaccgcaat
      541 ccgttcatcg actatcctca gttcatcgac cggatcacct cgatcagcac gctttccacc
      601 gctcccgtag tacgttcgct cgacccgatg caggatacga tcatctacgg ttcggtgctt
      661 cccgctgcta caacggttta taccttcgta gtggtaaaca atggcaacac agccgtgaac
      721 ttttccaact tcaacctgac gccaaccccc gtcctgtctt tcgcaggcac cggcgtcgat
      781 acgaccatcg gtcccggcga tgctctcgcg ttgcagatcg cctgcacgcc ttccaccatg
      841 tcggcggtca acggtttcct gaccttcaac accgacgtgc ccggcagtac gtcattctcg
      901 attccgatct acgcgaacga cccagtcttc agcacgatcg atgaaacgaa tcgtaaaagc
      961 ctgctcgttt tcccgaaccc ggcttccgac ctggtttggc tgacaaccga aagcatcacc
     1021 gacaccccgg tctttgtcta tgaccttgcc ggaagagctc tgggtcaact accgacacaa
     1081 caactcggtc accgcctgca actctccctc gccggactcg cgcccggctg ctacctcatc
     1141 ggcctgcaca ccatcgccgg cccggtgttt acaagagttg taaaggagta aatggttagt
     1201 aggaatcagc cgcatactgc tgac
//
LOCUS       DAOTON010000889         1221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_91706_length_1221_cov_3.458834, whole genome shotgun sequence.
ACCESSION   DAOTON010000889 DAOTON010000000
VERSION     DAOTON010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91706_length_1221_cov_3.458834"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1221
                     /locus_tag="P5292_13670"
     CDS             <1..>1221
                     /locus_tag="P5292_13670"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRS40223.1"
                     /translation="DGFARGDDEATTVAVDVLGNIFVTGYADWDTQQDNILLLKYDVS
                     GNLVWATNWNGSSYYDDMPTVIAIDPSGDVVVTGYTEPDTVTGSQDYITLKFASTNGS
                     LAWQAQYNRPGVTSGKDEAYGLAVDGLGDVYVTGRSSNGTDDDIVTIKYAGSTGIPAW
                     TVIYNSGNGDDRGLGLVLDNAGNVLVTGRNDNGNNDDIRTIKYNSNGVLQWSKFYNGP
                     ANQNDRGLAIAVDGSNNVYVTGQSDVDNSAVTDYDIVTVKYNASGVAQWARVEAGNAL
                     QDDIPSAIAVDGAGNAIVTGKSDQDPNALVTDNSYITILYNTAGVKQWTMLYAGSRTG
                     GSDISSSVCIGGSSIYIAGGSENTVTQKDATAIKYDYSGNEVWVRSYNGKGDFSESAR
                     AIVVDANNRSYAAG"
BASE COUNT          294 a          296 c          360 g          271 t
ORIGIN      
        1 acgacgggtt tgcaagaggg gatgatgaag cgactacggt agcggtcgac gttctgggga
       61 atatttttgt gaccggatat gccgactggg atacgcaaca ggataacatc ctgctgctca
      121 agtatgacgt gagtggaaat ctggtttggg ccaccaattg gaatggcagc tcttattacg
      181 atgatatgcc aacggtcatc gccatcgatc cttcaggcga cgtggtcgtg accggttaca
      241 cggaaccgga tacggtaacc ggcagccagg attacattac actcaagttc gcatccacga
      301 atggatcatt agcctggcag gcgcagtaca accgtcccgg tgttacgagt ggcaaagacg
      361 aagcctatgg ccttgcggtc gatgggctgg gagatgttta cgttaccggc cgcagctcca
      421 atggtacgga tgatgacatt gtcaccatca aatacgccgg aagtacgggc atcccggcct
      481 ggacggtcat ctacaattcc ggcaacggtg acgaccgggg gctcggtctt gtgctcgaca
      541 acgcgggcaa cgtactggtg accggtcgga atgataatgg caacaatgac gacatccgca
      601 ccatcaagta taattccaac ggcgtcctgc agtggagcaa gttctacaat ggccccgcca
      661 accagaacga tcgtggtctc gcgatcgcgg tggatggttc gaacaatgtg tatgtgacgg
      721 gtcagtcgga tgtcgacaat tccgccgtta ccgattatga cattgttacg gtaaagtaca
      781 atgcttcggg cgtcgcgcag tgggcgaggg tggaagccgg gaacgcgctg caggatgaca
      841 tcccttccgc catcgcggtg gatggggctg gtaacgcgat tgtgaccgga aagagcgacc
      901 aggatcccaa cgcgctcgtt accgacaact cctacataac tattttgtac aatacggccg
      961 gcgtgaaaca atggaccatg ttgtatgcag gtagtcgtac cggaggaagt gatatttctt
     1021 cctcggtttg tattggaggc tcttccattt acatcgcggg cggttccgag aatacggtca
     1081 cccaaaagga tgctaccgcg atcaaatatg attattccgg caatgaagta tgggtgagat
     1141 cctataacgg taagggggat ttttccgaaa gcgctcgcgc cattgtggtg gatgccaaca
     1201 accggtctta tgcagcaggt t
//
LOCUS       DAOTON010000890         1221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_91767_length_1221_cov_2.001715, whole genome shotgun sequence.
ACCESSION   DAOTON010000890 DAOTON010000000
VERSION     DAOTON010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91767_length_1221_cov_2.001715"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..>1221
                     /locus_tag="P5292_13675"
     CDS             164..>1221
                     /locus_tag="P5292_13675"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HRS40224.1"
                     /translation="MSISDPSVTAVLDLTYLGFHAMVMDPAGWYFIDPFTVGDSLHYI
                     VYFKRDARSGVPFECGSGTASQKLLPSNGSQVQRSAGTTLRTYRLAVGCTGEYAAFFG
                     GTVPGAMSGIVTSVNRVNAVYEQELAIRMVLVTNNDRVVYTNAATDPYTNSSGSTMLG
                     ENQTTLDSRIGSANYDIGHVFSTGGGGVASLRSPCTSSKARGVTGRGSPTGDAFDIDY
                     VAHEMGHQFGGNHTFNGNQGSCNGNRNASTAYEVGSGTTIMAYAGICGTDNTQSFSDP
                     YFHTISFDEIRNFVETGSGSTCGVASASGNQAPSCNGGANYTIPISTPFTLTGSGTDP
                     DGDRVTYLWEQFDLGAAGA"
BASE COUNT          266 a          332 c          347 g          276 t
ORIGIN      
        1 gcagcaagcg ccaatggaag tccccggaca accgttcgtg tcttccctgg tgatctcact
       61 tcccggtccg gatggccgtt cgatggattt caaagtccga gcatacgaaa tgatggagcc
      121 cggactaagc aggaagtttc ctgccctccg gacctatcag gctgtgagta tcagtgaccc
      181 aagtgtgacg gctgtactgg atctaaccta cctgggtttt catgcaatgg tgatggaccc
      241 ggccggctgg tattttatcg atccgttcac ggtcggtgat tcgctacact acatcgtcta
      301 tttcaaacgc gatgcgcggt ctggcgttcc attcgaatgc ggatcgggta ctgcatcgca
      361 gaaactctta ccgtcaaacg gttcacaggt acaacgatcc gcagggacca ccttgcgtac
      421 ctatcggctt gctgtcggtt gcaccggtga atatgctgct ttctttgggg gtacggtgcc
      481 cggtgccatg tcgggtatcg taacctcggt caatcgtgtg aatgcagtct atgaacagga
      541 gttggccatt cgcatggtcc tcgtgacaaa caacgatcgt gtggtttaca cgaatgcagc
      601 cacggatcct tacacgaata gttcgggttc gacgatgctg ggggagaacc agacgaccct
      661 cgattcgcgg atcggttcgg cgaattacga tatcggacac gtcttcagta ccggcggtgg
      721 cggagtcgca tcgctgcgtt caccctgcac ttcctcgaag gcacgtggcg tgacgggaag
      781 aggtagtccc acgggcgatg cattcgacat cgactacgtc gcccacgaaa tgggacacca
      841 gttcggaggc aaccatacct tcaacggcaa ccagggctct tgcaacggga accggaatgc
      901 ctcgacggcc tacgaggttg ggagtggaac gaccatcatg gcctacgcag gtatctgcgg
      961 aacggacaat acccaaagct ttagtgatcc gtatttccat accatcagct ttgatgaaat
     1021 caggaatttc gtcgagaccg gcagtggcag tacgtgcggt gttgcaagcg cttccggcaa
     1081 ccaggctcct tcctgcaatg gaggagcgaa ttatacgatt ccgatcagta caccctttac
     1141 cctcacaggc agcggaaccg atcccgatgg tgatcgggtt acttatctgt gggagcaatt
     1201 cgacctgggc gctgcaggcg c
//
LOCUS       DAOTON010000891         1220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_91850_length_1220_cov_3.038627, whole genome shotgun sequence.
ACCESSION   DAOTON010000891 DAOTON010000000
VERSION     DAOTON010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91850_length_1220_cov_3.038627"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="P5292_13680"
     CDS             complement(<1..181)
                     /locus_tag="P5292_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HRS40225.1"
                     /translation="MSEIKSQKKQKYTLEFEIKSSPKILFNYLSTASGLEEWFADKVN
                     IREGDYMFQWDGTEQR"
     gene            313..1056
                     /locus_tag="P5292_13685"
     CDS             313..1056
                     /locus_tag="P5292_13685"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRS40226.1"
                     /translation="MALINSLESDGNWLFRRRGQLPVVLFLLAIPAIAFTDYEQLSPA
                     FSHWMNWLAAGCSLLGFLVRAYTIGTTPRGTSGRNTAGQVAESLNSTGIYSLVRHPLY
                     LGNYLMWIGIVIFTKNIWFVIIVSLLYWLYYERIMFAEERFLERKFGDAYLKWASKLP
                     AFVPRLSGFVPSVIPFSVRSVLRREYSGVLATVIGFVFVEVMIGWFRWGTFSIGDHFL
                     VVLYVTIAMTLLLRSLKHYTSLLNEEGRS"
BASE COUNT          266 a          318 c          309 g          327 t
ORIGIN      
        1 cacgctgttc cgtaccgtcc cattgaaaca tgtaatcgcc ttcccgaata ttcactttgt
       61 cggcgaacca ttcctcaaga ccgctggcgg tactcaggta attgaaaagg atcttcggtg
      121 aagacttgat ctcgaattcg agggtatatt tctgcttctt ctgggacttg atttccgaca
      181 tcactagact acgttcaggg gccatagggc cagatttgag agggcgcaag atacctaaaa
      241 atacggttcg aacgacctga ttcgcatcat gaaaacgttc aaagccgatc tttattctat
      301 tttcgtgccc atatggcgct catcaactcg cttgaatcgg atggaaactg gctctttcgc
      361 agaaggggac aattgcccgt cgtcttgttt ctgctggcca ttccggcaat cgcgtttacg
      421 gactacgaac agctctcgcc ggcattcagt cattggatga actggctggc tgccggttgc
      481 agtctgttgg gattcctggt acgcgcgtac accatcggaa ccacgccgcg cggaacttcc
      541 ggcagaaata ccgccggaca agtagccgaa agcctgaaca gtaccggcat ttactcgctc
      601 gtgcgtcacc cgctttacct cggcaactac ctgatgtgga tcggcattgt gatatttacc
      661 aagaacatct ggtttgtcat catcgtcagt ttgctgtatt ggctctatta cgagcgaatc
      721 atgttcgccg aagaacgttt tctggaacga aagtttggcg atgcttacct gaaatgggcc
      781 agcaaacttc cggctttcgt tccccgactt tcgggatttg ttccaagtgt catcccgttt
      841 tccgttcgct cggtcctgcg ccgcgagtat tccggggtgc tcgccacggt gatcggattc
      901 gtcttcgtgg aagtgatgat cggctggttc cgctggggaa ccttctcgat cggtgatcac
      961 tttttggtgg tgctctatgt caccattgca atgaccttgt tactgcgaag cctgaaacac
     1021 tacaccagct tgctcaacga agagggacgt tcataaggta tcaagcagga gttgtatgaa
     1081 cttccccgtc gttcagccct atcgggcgat tcgttgatcg gagtcggaga accctccaca
     1141 aaaaataaca tcccgttcgg ttgaacggga tgttgatgta gcgagggagg gaatccctgt
     1201 ccgccgaacc ttccttgaat
//
LOCUS       DAOTON010000892         1220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_91860_length_1220_cov_2.802575, whole genome shotgun sequence.
ACCESSION   DAOTON010000892 DAOTON010000000
VERSION     DAOTON010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91860_length_1220_cov_2.802575"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="P5292_13690"
     CDS             complement(<1..611)
                     /locus_tag="P5292_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40227.1"
                     /translation="MAFYLTLSLYILAVLGGLILMISIRRVPRGTFNRLAVTHGLLGI
                     GAILSFVLVKNPDPEAPHYLLFAFIISGVLLSGLVWRVPTAFPVKIYFGLFALTFPLF
                     QFSPSMLVNFLLTFRYTDSLGKTFDLGNRYFIEEQQAVLRSKSTLRYKLVRKTGIYRS
                     TIARDIDFAGPVDSIRVLELSGNASISIRAYRIHNTYVSTEID"
     gene            656..>1220
                     /locus_tag="P5292_13695"
     CDS             656..>1220
                     /locus_tag="P5292_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40228.1"
                     /translation="MPQLRAILLLLLLNGLKMPAAIGSVGDTTMVRAFDHFLHQNCNS
                     GTGRFLFPSDSARFYKVMLHYQLSCPSFGCDIYDRIATLKLLRPTGEIDSTLTLAPSF
                     TVNGSRVDTLTCVLDTAYAYSYDTLSQSIDSVPLPGLTVLFYTDSLQPFTPTDTLTVW
                     PLYYYQYSFDASGNATDSTLIAPDSTFY"
BASE COUNT          289 a          314 c          313 g          304 t
ORIGIN      
        1 ctgtcgatct ccgtactcac ataggtgtta tgaatacgat aggctcgaat gctgatggag
       61 gcgtttcctg atagttccag gacgcggatg gaatcgaccg gtccggcaaa gtcgatgtcc
      121 cgtgcgatcg tactgcgata gatcccggtc ttccgtacga gtttgtatcg cagcgttgat
      181 ttggaacgca agaccgcctg ctgttcctcg atgaaatacc ggtttccgag gtcgaacgtt
      241 ttgcccaatg agtcggtgta acgaaaggtc aacagaaaat tgacaagcat ggaaggggaa
      301 aattggaaga gcggaaaggt gagtgcaaac aaaccgaaat agatttttac cgggaaggcc
      361 gttgggacgc gccagacgag ccccgacaac aggacaccgg aaatgataaa ggcgaagagc
      421 aggtaatgag gggcctccgg atcaggattc ttgaccagaa cgaaggatag gatggcgccg
      481 attcccagta gcccgtgggt aacagccaga cggttgaatg tcccccgggg tacccgtcga
      541 atgctgatca tcaggatcag tccgcccaga acggctaata tgtaaaggct gagcgtgaga
      601 taaaatgcca tgatccgggg tatctttctt aatttaccac ccaaagctat taaacatgcc
      661 tcaattgcgt gcgatcttat tgctgttgtt actcaatggg ttgaaaatgc ctgccgctat
      721 tggatcggtc ggcgatacga ccatggtccg tgcattcgac cattttctcc accagaattg
      781 taattccggt accggcaggt ttttgtttcc ttcggattcg gctcgctttt ataaagtaat
      841 gctccattac cagctgagtt gtccgtcttt cggatgcgac atctatgacc ggattgcaac
      901 cttgaaactc cttcgcccaa ccggagagat cgattcgact ttgacactgg cgccgtcctt
      961 taccgtgaac ggcagccggg ttgatacctt gacgtgcgtg ctcgatacgg cctatgcgta
     1021 cagctatgat accctcagtc aatcgatcga ttccgttcct ctgccgggac tgacggtgtt
     1081 gttttatacc gattccctac agccattcac gccgaccgat acgcttaccg tctggcccct
     1141 gtattattac cagtatagtt tcgacgcttc cggtaacgcc accgattcca ccctcattgc
     1201 cccggattct actttctacc
//
LOCUS       DAOTON010000893         1218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_92099_length_1218_cov_2.622528, whole genome shotgun sequence.
ACCESSION   DAOTON010000893 DAOTON010000000
VERSION     DAOTON010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92099_length_1218_cov_2.622528"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1218)
                     /locus_tag="P5292_13700"
     CDS             complement(<1..>1218)
                     /locus_tag="P5292_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40229.1"
                     /translation="PNVNAGADQTLTCTTTSITLIATSSTAGATFEWVGGPSGATKTV
                     SAAGTYTVIATNPANGCTASDEVVIGQDGNLPDVNAGADQSLSCTTTSITLTATSSTA
                     GATFAWVGGPSGASKTVTAAGTYTVVATNPANGCTASDEVVIGQDINTPNVNAGADQS
                     LTCTTTSITLTATSSTAGATFAWVGGPSGASKTVTAAGTYTVVATDPANGCTASDEVV
                     IGLNNNVPNANAGPDLTVGCGSGTVTLQGSSTTAGVDFSWTASNGGHIVSGGGSASPL
                     VDEAGTYTVVVTNPTNGCTASDFMIVTETQLPNCNISGNETICQGQSTNLCVGGGYAS
                     YQWSTGATTSCISVSQAGTYTVTVTNSDGCTSSCSKTVTVTGSTNCLISGNLNICQGQ
                     TTQLCAPAGYSSY"
BASE COUNT          231 a          339 c          389 g          259 t
ORIGIN      
        1 gataggaact gtagccggca ggagcacaca attgtgtcgt ctgaccctgg cagatgttga
       61 ggttgccgct gatcaggcaa ttggtgctgc ccgtgaccgt caccgtcttg ctgcagctac
      121 tggtgcaacc atcgctgttc gttaccgtga ccgtataggt gccggcctgg gaaacgctga
      181 tgcaggaagt agtcgcgccg gttgaccact ggtacgaggc gtagccgccg cctacacaga
      241 ggttggtcga ctgaccctga cagatcgttt catttccgga aatgttgcag ttcggcaatt
      301 gggtctcggt cacgatcatg aaatcggaag cggtacaacc gttcgtcggg ttcgtcacga
      361 caaccgtata ggtgccggct tcatccacca gcggagaggc ggagccgccg ccactcacga
      421 tatgcccgcc attggacgcg gtccaggaga aatcaacacc tgcggtcgtc gaagacccct
      481 gcagggtaac ggttccggag ccgcagccga ccgtcagatc aggtccggca ttggcgttcg
      541 ggacgttgtt gtttaatccg atcaccactt catcggaagc ggtgcagccg ttggccggat
      601 cagtggcgac cactgtgtag gtgccggcag ccgttacggt cttgctggca ccgctcggtc
      661 cgcctaccca ggcgaaggtc gcgcctgcag tggaggaggt agcagtaagt gtaatgctcg
      721 tggtggtaca ggtcaaggat tgatccgcac cggcgttgac gttcggtgtg ttgatgtcct
      781 gtccgattac cacttcatcc gaagcagtac aaccgttggc aggattggtg gcgacaaccg
      841 tgtacgtacc ggcagccgta acggtcttgc tggcgccact cggtccgcct acccatgcga
      901 aggtcgcacc ggcggtggaa gaggtggcgg tcagcgtgat gctggtcgtc gtacaggaca
      961 gcgactgatc agcgcctgca ttcacgtccg gtaagttgcc gtcttgcccg atgaccacct
     1021 cgtcggaagc ggtacagcca ttggcaggat tcgtggcgat caccgtgtag gttccggcag
     1081 cgctgacggt cttcgtagct ccactcggac caccaaccca ttcgaaggta gcgcctgcag
     1141 tggaagaggt cgctatcagg gtgatgctgg tagtggtaca ggtgagggtc tggtctgcgc
     1201 ctgcatttac attcggca
//
LOCUS       DAOTON010000894         1218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_92126_length_1218_cov_2.042132, whole genome shotgun sequence.
ACCESSION   DAOTON010000894 DAOTON010000000
VERSION     DAOTON010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92126_length_1218_cov_2.042132"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..577
                     /locus_tag="P5292_13705"
     CDS             71..577
                     /locus_tag="P5292_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40230.1"
                     /translation="MNRISILLFFLGLGTLAACKKQETYSLVPAIEYKSIFRAPATTG
                     FDSAVVVTISFTDGDGDIGYYPVGDSRNDPKFDDVNSQYYNNYVVKTYILESGVWQAD
                     PINVSARLPYMTPEGSNKALRGDIQRTLTLPPALNNDTLKYEVYIWDRALNRSNIITT
                     DAIVLRTQ"
     gene            complement(591..>1218)
                     /locus_tag="P5292_13710"
     CDS             complement(591..>1218)
                     /locus_tag="P5292_13710"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS40231.1"
                     /translation="ASVILSHQLYLPLPTSMQWAKGLVQRRLHRWLNRFGEIWVPDHP
                     GRENLSGGLSHPAPGNLRVEYIGPLSRFSTVSLQQVQQKTLDVVAVLSGPEPQRSLLE
                     SRIVARYQHTTMKVRIITGQPGVDRTRDSSGNIEFVGHLADEEFIDTLKRAGTIYCRS
                     GYSSLMDLDALGLKAVLIPTPGQTEQEYLAEHFRMKGWEVLSQSELSK"
BASE COUNT          285 a          333 c          321 g          279 t
ORIGIN      
        1 ggatcaatgc catagtagcg aatgggtaag cagaatcttt cgtaaattta accgttccct
       61 tccaatgacc ttgaatcgca tttcgatact gctgtttttc ctgggtcttg gaaccctggc
      121 cgcctgtaaa aagcaggaga cgtactcgct tgtacccgcg atcgagtaca aaagcatctt
      181 ccgggctccc gccacgaccg gcttcgattc ggccgtggtg gtgacgatct ccttcacgga
      241 cggagatggc gacatcggtt attatccggt cggcgatagc cggaacgacc cgaagttcga
      301 cgacgtgaac agccaatatt acaataacta tgtcgtaaag acctacattc ttgaatccgg
      361 tgtatggcag gctgatccga tcaacgtcag cgcccgattg ccctacatga ccccggaagg
      421 gagcaacaaa gcccttcgcg gggatatcca aagaaccctg acgttaccac cggccctgaa
      481 caacgacacc ttgaagtacg aggtgtacat ctgggaccgg gcgctgaatc gcagcaacat
      541 catcaccacg gatgccatcg tactccgaac acagtgatga atcgcacgcg ctactttgag
      601 agttccgact gcgagaggac ttcccatcct ttcattcgaa aatgttccgc caggtactcc
      661 tgttcggttt gaccaggtgt cgggatcaac acggccttta atcccaaggc atccagatcc
      721 atcaggctcg aataacctga gcgacaatag atcgttccgg cgcgtttcag ggtatcgata
      781 aattcctcgt ctgccaggtg cccgacgaat tcgatgttgc cggagctgtc tcgggtgcgg
      841 tcgacaccgg gttgacccgt aatgatccga actttcatcg ttgtatgctg gtagcgggca
      901 acgatccgtg attccagcaa actgcgttgt ggttccggtc cgctgaggac ggccactacg
      961 tccaaggtct tttgctgtac ttgctgcaag gaaacagtgc taaatcggga taacggaccg
     1021 atatattcga cccgaagatt cccgggagcc ggatgcgata gtcctccact aagattctct
     1081 cttcccggat ggtccgggac ccagatctcc ccgaagcggt ttaaccagcg gtgaagtctg
     1141 cgttgaacaa gtcccttggc ccattgcatg ctggtgggaa gtgggaggta cagttgatgc
     1201 gaaagtatga ccgatgct
//
LOCUS       DAOTON010000895         1214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_92549_length_1214_cov_3.430544, whole genome shotgun sequence.
ACCESSION   DAOTON010000895 DAOTON010000000
VERSION     DAOTON010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92549_length_1214_cov_3.430544"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="P5292_13715"
     CDS             <1..622
                     /locus_tag="P5292_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRS40232.1"
                     /translation="NSKTAEMCKLVENAYRDVNIAFANELSIICDKAGIDVNELIDLA
                     SKHPRVKILRPGPGVGGHCIAVDPWFIVSEFPSDARIIRLARQINNNKRDWVLKKIRS
                     QANEFKKKNGRAPVIACMGLSYKPDIDDLRESPALEIFHDLEKERFRVMAVEPNLRKH
                     PELEITDTLKAVGEADIIVYLVAHKEFRNLDPGSGKIILDFCGIAR"
     gene            699..>1214
                     /locus_tag="P5292_13720"
     CDS             699..>1214
                     /locus_tag="P5292_13720"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRS40233.1"
                     /translation="MISPNVRNTLLLRVSGLGLIYLLQVLLARLMGPKGFGDFTVIQA
                     WLNVLLVVSIFGFDTAALRFLPSLFKAKSFSKAQGFLKFSFRFITIMAIVCAVIAFIF
                     LLTTAKKSSVSFAEGFFWSLFLLPILAFIYQGSAVLRSLQRTNLAIVPVYLMLPVLLG
                     ITSLLHYYKYGR"
BASE COUNT          255 a          325 c          322 g          312 t
ORIGIN      
        1 caactcaaag accgccgaga tgtgcaaact ggtagagaac gcttaccgcg acgtgaacat
       61 cgccttcgcg aacgagctct ccatcatctg cgataaagcc gggatcgatg tgaatgaact
      121 catcgacctt gccagtaagc acccacgggt gaagatcctg cgccccgggc cgggtgtcgg
      181 ggggcactgc attgcggtcg atccgtggtt cattgtttct gaattcccat ccgatgcgcg
      241 catcatccgg ctcgcccgtc agatcaacaa taacaagcgg gattgggtat tgaagaagat
      301 ccgttcacag gcgaacgagt tcaagaaaaa gaacggacgg gcgccggtga tagcctgcat
      361 gggtctgagc tataaacccg atatcgacga tcttcgtgaa tctccggcat tggagatctt
      421 tcatgatctg gaaaaggaac gattccgggt aatggccgtg gaacccaatc tccgcaaaca
      481 cccggaactt gagatcaccg atacgctcaa ggctgtcgga gaggcggata tcattgtcta
      541 cctcgtcgcg cacaaggaat tccggaatct tgatcccggt agcggtaaga tcatcctcga
      601 cttctgcggc atcgcccgtt gatggtgagg tacggttcgc tcgaattcac cgtcccgaac
      661 ccgatccggt aaggaccggg ttcttcgttt gattgctgat gatatcgccc aacgtccgaa
      721 atacactgct gctgcgggtt tcaggtttag gcctgatcta cctcttgcag gtattgttgg
      781 cccggctcat gggcccgaag ggcttcggtg attttaccgt catacaagcc tggttgaacg
      841 tcttgctggt cgtcagcatc ttcggatttg atacggccgc gctgcggttc ctgccttccc
      901 tgttcaaggc aaagtccttc tccaaggctc agggcttttt gaagttctcg tttcgcttca
      961 tcaccatcat ggcgattgta tgtgcggtga tagcattcat cttcctgttg actaccgcta
     1021 agaaaagttc cgtgagcttt gcggaaggtt tcttctggtc cttgttcctt ttacccatcc
     1081 tggcgttcat ttatcagggc agtgccgtac tacgctcctt gcagcgtacg aacctggcca
     1141 ttgttccggt gtacctgatg ttgccggtct tgctcgggat cacttcactt ctgcattact
     1201 ataagtatgg aagg
//
LOCUS       DAOTON010000896         1214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_92570_length_1214_cov_2.762726, whole genome shotgun sequence.
ACCESSION   DAOTON010000896 DAOTON010000000
VERSION     DAOTON010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92570_length_1214_cov_2.762726"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="P5292_13725"
     CDS             complement(<1..629)
                     /locus_tag="P5292_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HRS40234.1"
                     /translation="MTTSKLKQTMAEIVRMPKMSDTMTEGVVAKWLKKVGDSIKPGDV
                     VAEIETDKATMEFESYQEGVLLYQGVSEGKAAPVDSVLAVLGKAGEEYQSLLDAEAAK
                     AVIKAPVAQPAAPAAAAPAPKPAPVSTPAPAPAPQPARPVAPVAASSNGDQRMKVSPL
                     AKRLANERGIDLSQVQGSGEHGRIVKRDIDWFKPGSFVASGAAAVGALT"
     gene            complement(655..>1214)
                     /locus_tag="P5292_13730"
     CDS             complement(655..>1214)
                     /locus_tag="P5292_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRS40235.1"
                     /translation="GDGATRQGALHEAFNMAMTWLLPVVYIIENNNYAMGTSVERTSN
                     VQELYKLGLSYDMPSEPVDGMSVEAVHEAMERAVNHARSGKGPYLLEINTYRYKGHSM
                     SDPAKYRTKEEVEEYKAKDPIEQVLITIRSKKYATEAEIETINTRVRDTVEDSVKFAE
                     ESPWPDPAELYTDVYVQADYPYIKE"
BASE COUNT          228 a          360 c          330 g          296 t
ORIGIN      
        1 tgtgtcaatg ctccgacagc ggcagcgccg ctggcaacga acgatcccgg cttgaaccag
       61 tcgatgtcgc gcttgacaat gcggccgtgt tcgccggatc cctgcacctg ggaaaggtcg
      121 attccgcgct cattcgccag tcgcttcgca agcggagaaa ctttcatgcg ctggtcgcca
      181 ttcgagcttg cggcaacagg agcaaccgga cgtgccggct gcggagcagg tgcgggtgcc
      241 ggtgtgctga caggagccgg tttcggcgca ggagcggcgg cggcaggagc agccggttgg
      301 gctaccggag ccttgatgac cgctttggcc gcttcggcat cgagcagtga ttggtattct
      361 tcgcccgctt tgcccagtac ggcaagtacg ctatcgaccg gtgcggcctt cccttcggaa
      421 acgccctggt acaataacac accttcctgg taagactcga attccatcgt agccttatcg
      481 gtctcaatct cggcaaccac gtcgccgggt ttaatggaat cccctacttt tttaagccac
      541 ttggcgacca cgccttcggt catcgtgtcg ctcatcttgg gcatccgtac gatctctgcc
      601 atcgtttgct ttagcttgct ggtggtcatt cgtcagtaac gaacgacacg tgaatcattc
      661 tttgatataa ggataatcag cctgaacgta aacgtccgta tacaattccg ccgggtccgg
      721 ccagggcgat tcctccgcaa attttaccga atcctctacc gtatcgcgca cgcgggtgtt
      781 gatcgtttcg atctccgctt cggtcgcgta cttcttggaa cggatcgtga tcaacacctg
      841 ttcgatcgga tctttcgcct tgtactcttc cacctcttcc ttcgtccggt acttggcggg
      901 atcgctcatc gaatgaccct tgtaacggta ggtattaatc tcaagcaggt aaggaccctt
      961 tcctgaacgc gcgtgattca cggcgcgttc catggcttcg tgaaccgctt ccacactcat
     1021 tccgtccacc ggctcggagg gcatatcgta cgaaagaccg agtttataca gttcctgcac
     1081 gttgctggtc cgttcgacgg atgtacccat cgcatagttg ttgttctcga taatatagac
     1141 gaccggaagc aaccaggtca tggccatgtt gaaggcctcg tgcaaggcac cctggcgtgt
     1201 ggcgccgtcg ccca
//
LOCUS       DAOTON010000897         1212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_92838_length_1212_cov_2.286085, whole genome shotgun sequence.
ACCESSION   DAOTON010000897 DAOTON010000000
VERSION     DAOTON010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92838_length_1212_cov_2.286085"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="P5292_13735"
     CDS             <1..978
                     /locus_tag="P5292_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="organic solvent tolerance protein OstA"
                     /protein_id="HRS40236.1"
                     /translation="FLGPTRINSSSNTMYCEQGWYNTNSQQTSFQGNSYLLTRTQMLR
                     GDSILYDRSSGIGRVFGHVSITDTVNRLIIRGDYGEHHELTDSSWVTGNAELVQIFDT
                     DSLFMHADTLLATAADSLPAASGDTAKQNNRNLFAFHHVRLFKPDLQGRCDSIAYTLK
                     DSTLRMFRDPVLWSGLNQLTADSIDIIAAGGGIHRLYLVNSAFIASRADSSQTGPIDS
                     LRFNQVRGKTMTGYFIDNNLYRIDVSGNGQTIYYTRNKEEKNFAVNRADCSDMTIYLE
                     ENKVKGLSLLQSPEGVLYPVKQISTRELRLKGFNWKEAQRPNDRAAIFR"
     gene            complement(982..>1212)
                     /locus_tag="P5292_13740"
     CDS             complement(982..>1212)
                     /locus_tag="P5292_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRS40237.1"
                     /translation="QGVAILLYGILVISVVDNVFRFVFQKKFADVHPLITEIGVIVGL
                     QLFGVPGIIFGPLLISYFLIMLRIFREEFLSV"
BASE COUNT          315 a          355 c          299 g          243 t
ORIGIN      
        1 ttcctgggac ccacccgtat taactcctcc agcaatacga tgtattgcga acaaggttgg
       61 tacaacacca acagtcagca gaccagcttt cagggaaatt cttatctcct gacccggacg
      121 cagatgcttc gtggagattc catactctat gaccgcagca gcgggatcgg ccgggtattc
      181 ggccatgtca gcatcaccga taccgtcaac cgcctgatca tccgcggaga ctacggggaa
      241 caccatgaac taacggatag ttcctgggtg accggaaatg ccgagttggt gcagatcttc
      301 gataccgaca gcctgttcat gcatgccgac accctcctgg ccaccgctgc cgattcactc
      361 cccgccgctt caggcgatac ggcaaagcag aacaaccgga acttgttcgc gttccatcat
      421 gtgcgcctgt ttaaaccgga cctgcaaggt cgttgcgatt ccatcgcgta tacactgaag
      481 gattcgaccc ttcggatgtt ccgggatcct gtcctctggt cggggctcaa ccaactcact
      541 gccgactcga tcgacatcat cgcagccggc ggcggaattc accggctgta cctggtcaat
      601 tctgcattca tcgcgtcacg tgccgacagc agtcaaacag ggccgatcga ttccttgcgc
      661 ttcaaccagg tcagaggtaa gaccatgacc ggctacttta tcgataataa tctctaccgg
      721 atcgacgtct ccggcaacgg tcagaccatt tattacacac gcaacaagga agaaaagaac
      781 ttcgccgtca accgggccga ttgcagcgac atgaccatct acctcgaaga aaataaggtc
      841 aaaggacttt ccctgctcca gtccccggag ggcgtattgt acccggtcaa gcagatcagt
      901 acccgggaac tgcgcctgaa aggttttaac tggaaggaag cccagcgtcc gaacgaccgg
      961 gcagcgatct ttcgataatc gtcagacgga gaggaattcc tcccggaata tccgcaacat
     1021 gatcaggaaa taggagatca gcagcggacc gaaaatgatt ccgggaacgc cgaacaattg
     1081 caatccgaca attaccccga tctcggtgat caggggatgt acatccgcga actttttctg
     1141 gaaaacgaaa cggaagacat tatccaccac ggagatcacg agaataccat agagcaggat
     1201 cgcgacaccc tg
//
LOCUS       DAOTON010000898         1211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_92924_length_1211_cov_3.044118, whole genome shotgun sequence.
ACCESSION   DAOTON010000898 DAOTON010000000
VERSION     DAOTON010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92924_length_1211_cov_3.044118"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1211)
                     /locus_tag="P5292_13745"
     CDS             complement(<1..>1211)
                     /locus_tag="P5292_13745"
                     /inference="COORDINATES: protein motif:HMM:NF037694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRS40238.1"
                     /translation="RFSNLRNLLGTFVPAMVTVRHDGDFNYLYFNDARVNTARYDVGG
                     RRSNSTGQFAFLYGDRDLYRPGETVHLNTIVRDEAWKPVADVPVKVRLVQPNGKEFRM
                     VRGQLSKQGSFSTDIPVPEAAMTGRYHAEIYTANDVLIAGSDIQIEEFMPDRIDVMVQ
                     LSKPSILSNEGLDVGISAVNFFGPPAANRRYEVEFSVVRKVFRPKGFDGYDFTVKTEN
                     GAALLSNDLHEGTTDENGKATVAFPAHPEWKDEGLLQGNAYVTVTDENGRPVNRTETF
                     PIHTQEVYFGAKLTEYYVNRGERFRIPVVAVNTEGKAVGASARIQVVRFDWYSAIERE
                     EYGSRYRYISHKKERMVFDQTVNIPAAGFDFVYTPAESGEYEIRVSGPDAEHGFSKNF
                     YAFGWGYTTNTS"
BASE COUNT          249 a          350 c          336 g          276 t
ORIGIN      
        1 gacgtgttgg tggtgtatcc ccatccgaac gcgtagaagt tcttcgagaa accatgttca
       61 gcgtccggac cgctgacccg gatctcatac tcgccggatt ccgccggtgt atagacgaag
      121 tcgaaacccg ctgcaggaat attgaccgtt tgatcgaaga ccatacgctc cttcttgtgc
      181 gagatgtagc gataacggct gccatactct tcgcgttcaa tcgcgctgta ccagtcaaaa
      241 cggaccacct ggatcctcgc gcttgctccg accgctttac cttcggtatt caccgccact
      301 accggaatcc tgaaccgttc gccgcggttg acatagtact cggtcaactt tgcaccgaag
      361 taaacctcct gcgtatggat cggaaacgtt tcggtgcggt tcaccgggcg accgttctca
      421 tcggtgacgg ttacatacgc gttaccctgg agcagtcctt cgtccttcca ttccggatgt
      481 gccgggaatg ccacggtggc cttgccgttc tcatccgtcg tgccttcgtg cagatcgtta
      541 ctcaggagag cagctccgtt ctcggtcttg acggtgaaat catatccgtc gaatcccttc
      601 gggcgaaaga ccttgcggac cacgctgaac tccacttcat accggcggtt agccgcaggc
      661 ggcccgaaga aattcacggc tgagatgccg acatccagtc cttcgtttga aaggatcgag
      721 ggttttgaca gttgcaccat cacgtcgatc cggtcgggca tgaattcctc gatctgaatg
      781 tcggatccgg cgatgaggac atcgttcgcc gtgtagattt ccgcgtgata gcgcccggtc
      841 atggccgcct ccggaacagg aatgtcggtc gaaaaggatc cctgcttgct gagctgacca
      901 cgcaccatgc ggaattcctt tccattcggt tgtacgagcc gcaccttgac cggcacatcc
      961 gccaccggct tccaggcctc atcgcgtacg atggtgttca ggtgtacggt ctcgccagga
     1021 cggtatagat cgcgatcgcc gtaaaggaaa gcgaactgtc cggtggaatt ggaacggcgg
     1081 ccgcccacat cgtaccgtgc ggtgttgact cgggcatcgt tgaagtagag gtaattgaaa
     1141 tcaccgtcgt gtcgaaccgt taccatggcc gggacaaaag tgccgaggag gttacgcaga
     1201 ttggaaaagc g
//
LOCUS       DAOTON010000899         1210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_93044_length_1210_cov_2.771429, whole genome shotgun sequence.
ACCESSION   DAOTON010000899 DAOTON010000000
VERSION     DAOTON010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93044_length_1210_cov_2.771429"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="P5292_13750"
     CDS             <1..624
                     /locus_tag="P5292_13750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40239.1"
                     /translation="LADSGRCYRFSFQHMLETEQFQDGGTVEYSLDRGWTWQTLGAAG
                     DPGWFNAGYITGLGLPPVAGFTGSVAQWTPASHDLVLDEPSEIVFRFRFGSDASIQMN
                     GWAIDQVCFSPVATCTIGLDEPNFDDIVSGPYPNPATTSASLVFRQGIGRCSVQLTDV
                     RGQVLSLPHAQESPDRIDLPLTGLSPGIYIISVDVDGRRLYRRLIVQ"
     gene            complement(698..>1210)
                     /gene="glyA"
                     /locus_tag="P5292_13755"
     CDS             complement(698..>1210)
                     /gene="glyA"
                     /locus_tag="P5292_13755"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476316.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRS40240.1"
                     /translation="MMSAVLDGAVFPGTQGGPLEHVIAGKAVAFGEDLQDDYLKYCVQ
                     VVKNAQAMSHAFMELGYKVISGGTDNHSMLIDLRSKDLTGKLAEQVLGKVSITVNKNM
                     VPFDDKSPFVTSGIRIGTPAMTTRGIKEKDAVRIVQLIDEALMNHDKETKLGSVLKKV
                     HTLTDKFVLY"
BASE COUNT          235 a          320 c          335 g          320 t
ORIGIN      
        1 cttgccgact ccggtcgatg ttaccggttc agctttcagc acatgttgga aacggaacaa
       61 ttccaggacg gaggaacggt ggagtacagt ctggatcgcg gttggacctg gcagaccctg
      121 ggcgccgccg gtgatccggg ctggttcaat gccggctata taaccggact tggattgcct
      181 cccgtcgcag gattcactgg ttcagttgcg caatggacac cggccagtca cgacctggtg
      241 ctggatgaac catcggaaat cgtcttccgt ttccgtttcg gttctgatgc ttcgattcaa
      301 atgaacggtt gggcgatcga tcaggtttgt ttcagccccg ttgctacctg tacgatcggt
      361 ctcgatgagc cgaatttcga tgacatcgta tcaggaccct atccgaatcc tgcgaccacg
      421 agcgcaagcc tcgtcttccg tcaaggcatc ggacggtgtt cggtgcagtt gacggatgtt
      481 cgaggtcagg ttttgtcgct tccgcatgcg caggaaagcc cggatcggat cgatcttccg
      541 ctgaccggtt tgtctcctgg tatttatatc atttccgtgg atgttgacgg aaggcgcctt
      601 taccgccgtc tgatcgtcca ataagcaatc gacccgactg acattgtcaa tggacaagtt
      661 caatcgggtc aatggttggt tttaaggaag tgtgtaatca gtacaacaca aacttgtcgg
      721 taagcgtatg cacttttttc aggacgctgc ccagcttcgt ttccttatca tgattcatca
      781 acgcctcgtc aatcaattgt acgatgcgca cggcgtcttt ttctttgatg ccgcgggtgg
      841 tcatggccgg ggtgccgatc cggatgccgg atgtgacaaa gggtgacttg tcgtcgaagg
      901 gcaccatgtt cttgttaacc gtgatgctga ctttgccaag tacctgttcc gccagcttcc
      961 cggtcagatc cttgctgcgc aggtcgatca acatgctgtg attgtcggtt cctccggaga
     1021 tcaccttgta accgagttcc atgaatgcat gcgacatggc ttgcgcgttc ttcacgactt
     1081 gcacgcaata cttcaggtaa tcgtcttgaa ggtcttcgcc gaaggcgact gctttgccgg
     1141 cgataacgtg ctcgagtggt ccgccctgtg tgccggggaa aacggctccg tcgagtaccg
     1201 cactcatcat
//
LOCUS       DAOTON010000900         1209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_93179_length_1209_cov_2.658579, whole genome shotgun sequence.
ACCESSION   DAOTON010000900 DAOTON010000000
VERSION     DAOTON010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93179_length_1209_cov_2.658579"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..873)
                     /locus_tag="P5292_13760"
     CDS             complement(154..873)
                     /locus_tag="P5292_13760"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRS40241.1"
                     /translation="MKPTRLFISILLLLSIATLSSCRKDWNGVRGDGPILTEDRIVSS
                     FHGVEYHLEGDVNIRRDSIFSVTVSSYGNYLPLIRTEVIGGKLIINSSKSLRDNEITV
                     DISMPSLDHLSLSGSGDIRTIDRFSDASLYVNLSGSGKVSYFGDVDDLRANISGSGMM
                     DLDGTANDATMTMSGSGKIAAFGMITNNNSATISGSGNIETFVSNHLDATISGSGTIR
                     YRGNPSVTTHISGSGSVVRVP"
     gene            complement(942..>1209)
                     /locus_tag="P5292_13765"
     CDS             complement(942..>1209)
                     /locus_tag="P5292_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40242.1"
                     /translation="TMHQPMQENHLHQFNPLTKFNLTAEKRLSKYFSLAAGPSLNVFY
                     AHLDDPDYAQVFSQLPPASLLDRQTIGNYDRSIWLGGKIALRFF"
BASE COUNT          271 a          297 c          336 g          305 t
ORIGIN      
        1 gtttcctgtt tcctgtttcc tgtttcctgt ttcctgtttc ctgttaatac caccacttca
       61 gcgagcagga ccttactatt taccattcac cactattcgc tattcgctat tcgctattca
      121 ctgttcactg tttactgttt actgttcact ccctcaaggc actctcacca ccgaaccaga
      181 acccgagatg tgcgtcgtaa ccgaagggtt gccgcggtag cggatcgtgc cggatccgga
      241 gatggtggcg tcgaggtgat tgctgacaaa cgtttcaatg ttgccggagc ccgaaatggt
      301 agcggagttg ttgttggtga tcatgccgaa cgcggcgatc tttcctgaac cgctcatggt
      361 catggtcgcg tcgttggcag taccgtcgag gtccatcatg ccactgccgg aaatgttggc
      421 gcgcaagtcg tctacgtctc cgaagtaact caccttaccc gatccgctca gattgacata
      481 cagggacgcg tcggagaaac ggtcgatcgt acggatgtcg cccgagccgg aaagcgagag
      541 gtgatcgagc gagggcattg agatatcgac agtgatctcg ttgtcgcgga gactttttga
      601 cgagttgatg atcagtttcc cgccgatcac ttccgtacgg atcagcggca ggtagttgcc
      661 atagctgcta acggttactg agaagatgga atcacggcgg atgtttacat caccttccag
      721 gtgatattcg acaccgtgga aggaagagac gatgcggtct tccgtcagga tgggaccatc
      781 accacgaacg ccgttccagt ctttacggca cgagctgagc gttgcaatgg acagtaggag
      841 caaaatgctg atgaaaaggc gggtaggttt catgggcttg ttctgtttat atacagaaga
      901 caagtccaag gggtcttacc cctatccctc gcgaaaaaaa atcagaagaa gcgcagcgct
      961 attttccctc ccagccagat gctgcgatcg tagttcccga tcgtctgtcg gtcgagtagg
     1021 gacgcaggcg gcaattggct gaaaacctgc gcataatcgg ggtcgtcgag gtgggcgtaa
     1081 aaaacgttca ggctgggccc cgctgcgagc gaaaagtatt ttgaaagacg cttttcagcc
     1141 gttaggttaa atttcgttaa ggggttgaat tgatggagat gattctcctg catcggttga
     1201 tgcatggta
//
LOCUS       DAOTON010000901         1205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_93677_length_1205_cov_2.780870, whole genome shotgun sequence.
ACCESSION   DAOTON010000901 DAOTON010000000
VERSION     DAOTON010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93677_length_1205_cov_2.780870"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="P5292_13770"
     CDS             complement(<1..672)
                     /locus_tag="P5292_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40243.1"
                     /translation="MKKFIQPLVVDNTPVVDLKADPYIQREGIVTLIPAKSNNDRTLS
                     FTFTGFTGKHYKTGETMWFGDVVKVNDDGSYKFRRITITDVREFNLNNPQDAAEWHII
                     RHHSNVKGSPNERSATWKVIDRFEEARQSLQQGQELRNFIDKILNMSGDQVKDLGRPF
                     NLNAEFNPVDVIRGELIRIATTNPNRLAKYFKDKDYFASMSVLHRCLAAGKVRNEPSR
                     GYIFND"
     gene            complement(745..>1205)
                     /locus_tag="P5292_13775"
     CDS             complement(745..>1205)
                     /locus_tag="P5292_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40244.1"
                     /translation="QLTAANALTANQRAVSGCTGTVLTGPEAVARGLIAAPLGNTAFN
                     IAGNKLIFVKNVGWDDPYKWYEVNNYNKSVRGNMWLFLHPGQVDRPNIEIISKGAYGI
                     NRSMVEAYLNGLTGGSEKPLHSVDAVSFEMLKEDMIVIYNTMACGILNAS"
BASE COUNT          250 a          308 c          330 g          317 t
ORIGIN      
        1 atcattgaag atgtatccgc gtgaaggctc gttgcgcact ttgccagcag cgaggcaccg
       61 gtgaagaacc gacatagatg caaagtagtc cttgtctttg aaatactttg ccaggcggtt
      121 gggattggta gttgcaatcc gaatcagctc tccacgaatg acatcgaccg ggttgaattc
      181 tgcattcagg ttgaacggcc ggcccagatc tttgacctgg tcgcccgaca tgttcagaat
      241 cttgtcgatg aaattgcgga gctcctggcc ttgttgcaag ctttggcgag cttcttcaaa
      301 gcgatcgatg actttccacg tagcgcttcg ctcgtttggc gagcctttga cgttgctgtg
      361 atgccgtatg atgtgccatt ctgcagcatc ttgcggattg ttcaggttga actcccgtac
      421 gtcagtaatg gtgatccttc ggaacttgta ggatccatca tcgttgactt taacaacatc
      481 tccaaaccac atcgtttcac ccgttttgta gtgctttccg gtaaaaccgg taaaggtgaa
      541 actcagcgtt ctgtcattgt tgctttttgc gggaatcagc gtaacaatgc cttcccgttg
      601 aatatacgga tctgctttta agtcgaccac cggagtatta tcaaccacca ggggttgaat
      661 gaattttttc atggctgttg ttgttaatag ttgagcacca gggcattaaa gaaagaagcg
      721 ggggccgaaa cccccgtctt ctcattagga cgcgttgagt atgccgcacg ccatcgtatt
      781 gtagatgacg atcatgtctt ccttcagcat ttcgaaggaa acggcatcca cggagtgcag
      841 cggcttttcg cttccgccag tcagaccgtt gaggtacgct tcaaccatcg agcgattgat
      901 gccataggca cctttgctga tgatctcgat gttgggtcgg tccacctggc ccggatggag
      961 aaacagccac atgttaccgc gaacggactt gttgtagttg ttcacctcgt accacttgta
     1021 cggatcgtcc caacccacgt tcttgacgaa gatcagcttg ttgccggcga tgttgaaggc
     1081 cgtgttgccg agcggggcgg cgatgaggcc gcgcgcgacc gcttccgggc ccgtgaggac
     1141 ggttcccgtg cacccggaga cggcgcgctg gttcgccgtg agcgcattgg cggccgtcag
     1201 ctggc
//
LOCUS       DAOTON010000902         1205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_93709_length_1205_cov_1.991304, whole genome shotgun sequence.
ACCESSION   DAOTON010000902 DAOTON010000000
VERSION     DAOTON010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93709_length_1205_cov_1.991304"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1205
                     /locus_tag="P5292_13780"
     CDS             <1..>1205
                     /locus_tag="P5292_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRS40245.1"
                     /translation="DNPSLTLVFSAGNNAQGDCGYGAGTPWGTISGGFKIGKNVIAVG
                     NLDAYEVIDPSSSHGPADDGRVKPDICANGRDQLSTDENNTYQLGGGTSAACPSVAGT
                     TAQLYQAYRELNSGSDPESPLIKATLLNTAEDIGNDGPDYSYGYGRINARRALQTLAN
                     RTYVLDSVGQGSSRNISITVPSGVRQLRVLLYWLDPAGDPSAAFQLVNDLDLTLSHAS
                     IGILQPWILDPTPNASTIGLPAVRGADHLNNVEQVTLDNPTAGTFTASVSGNVVPSGV
                     QKFYVVWEFRTDEIVLTYPTGGEGFAIGSEELLRWDAYGVSTPFTLEFSPDNGANWQP
                     IATVGPDIRQYAWTPIASQRSGEALVRITRGSDSDLSDETFTVSGIPQNLHVDFACPD
                     TMQIGWNAV"
BASE COUNT          258 a          350 c          333 g          264 t
ORIGIN      
        1 acgataatcc atccctcaca ctggtctttt ccgccgggaa caatgcccag ggcgattgcg
       61 gctacggcgc cggtactccc tggggtacga tcagcggcgg cttcaagatc ggcaagaacg
      121 tgatcgctgt tggcaacctc gatgcctacg aggtgatcga tcccagcagt tcccacggcc
      181 cggccgacga tggtcgtgtc aaaccggaca tctgcgccaa tggccgcgac cagctttcca
      241 ccgacgagaa caatacctac cagctcggag gaggcaccag cgctgcctgt ccttccgttg
      301 ccggaacaac cgctcaactg taccaggcct atcgtgagct gaacagtgga agtgaccccg
      361 agtctccact tatcaaggct accctcttga acactgcgga agatatcggc aatgacggac
      421 ctgattacag ttacggatat ggccgtatca atgcacgtcg ggcgttacag acattggcga
      481 accggacgta tgtactcgat tcggttggcc agggctcctc acgcaacatc tccattacgg
      541 tgccttccgg tgtccgccag cttcgcgtgt tgttgtactg gttggatccg gctggagatc
      601 cctccgcggc gtttcaactg gtgaatgacc tggatctgac cctgagccat gcgtccattg
      661 gcattttgca accctggata ctcgatccga cgccgaatgc ttcaaccatt ggtctgccgg
      721 ctgtacgtgg agccgatcac ctcaataacg tagaacaggt aacactcgat aatcccacgg
      781 ccggaacctt caccgcaagc gtgtcgggga acgttgtacc gtccggagtg cagaagtttt
      841 atgtggtatg ggaattccga acggatgaga tcgtgttaac gtacccgacc ggtggcgaag
      901 gctttgctat aggctcggaa gaattgctgc gctgggatgc ctacggcgtt tcgacgccgt
      961 tcacactcga gttttcgccg gacaatggcg cgaactggca accgatcgct accgttggcc
     1021 ccgatattcg tcaatacgca tggacaccca tcgcttccca gcgtagcggc gaagcattgg
     1081 ttcggattac acgtggttcg gattcagacc tgtcggatga gacgttcacg gtaagcggta
     1141 tcccacaaaa tcttcacgtg gattttgcct gcccggatac catgcagatc ggatggaatg
     1201 ccgta
//
LOCUS       DAOTON010000903         1199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_94486_length_1199_cov_1.683566, whole genome shotgun sequence.
ACCESSION   DAOTON010000903 DAOTON010000000
VERSION     DAOTON010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94486_length_1199_cov_1.683566"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="P5292_13785"
     CDS             <1..280
                     /locus_tag="P5292_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRS40246.1"
                     /translation="RGAALSKGGKAIIALPSRTQKGVSRITPFLKQGGGVVTTRAHVH
                     YVVTEHGIAYLFGKNLRERARELIRIAHPDDRELLEQAARDRFGRWSD"
     gene            325..>1199
                     /locus_tag="P5292_13790"
     CDS             325..>1199
                     /locus_tag="P5292_13790"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRS40247.1"
                     /translation="MRQFLFLVFSIVSAGLHLATAQTAGQQLWSQAVVHDVYLQFHQL
                     SYLDTLSTNYTADVYTVCDLTIDGQVLSNSGAKYKGNSSYNNPGPKKSFKLDLIEFVS
                     GQEYDGIKKFNLNNGFKDPSFLREKLMLDFLVSHDIAAPRCSYARVFVNGTYWGLYTL
                     VEEVNNDFCDRWFGSSSGNRFKGDPSGDLRWYGSSPNLYYNRYELDNNETANDWSDLI
                     RLIDKINNSGTAFHDSLAEVLNTWPFFRHWAADNLFANLDSYIGSGHNYFIYHESVSD
                     QFEWIHWDVNEAFGN"
BASE COUNT          289 a          327 c          292 g          291 t
ORIGIN      
        1 gcgtggcgcc gccttgtcaa agggcggaaa agccatcatc gcgctaccct cgcgtacaca
       61 gaaaggtgtt tcccggataa cgccctttct gaagcaaggc ggcggggtgg taacgacgcg
      121 cgctcacgtc cactatgtgg tcaccgaaca cgggattgcc tacctgttcg ggaagaacct
      181 gcgggaacgc gcccgcgaat tgatccgcat cgctcatccc gatgaccgcg aactgctcga
      241 gcaggccgcg cgtgatcgat ttgggcgatg gtcggattaa acccagctac ccattcgatg
      301 tccaacggac ttccatcacc cgccatgcgt caatttctgt tcctggtatt ttcgatcgtg
      361 tccgccggat tgcatcttgc aacggcccaa acagccggac aacagctttg gtcgcaagcc
      421 gtcgttcatg atgtgtacct gcaattccac cagctttcct atctcgatac gctgtcgacc
      481 aattatacag cggatgttta taccgtatgc gacctgacca tagacggcca agtgctttct
      541 aacagcggcg ctaaatacaa aggcaatagc tcttataaca atcccggacc gaagaaatct
      601 ttcaagcttg atttgatcga attcgtatcc ggtcaggaat atgatggcat caagaaattc
      661 aacctgaaca atggcttcaa agatcccagc tttctccggg aaaaactgat gctcgatttt
      721 ctggtttcac acgacatagc ggcacccaga tgctcctatg ccagggtctt cgtcaatggc
      781 acctattggg gtttatacac tttggtcgaa gaggtcaata acgacttttg cgaccgttgg
      841 ttcggtagca gttccggcaa ccggttcaag ggtgatcctt ccggtgacct gcgctggtat
      901 ggaagcagtc cgaacttgta ttacaaccgt tatgaactgg ataataacga aacggccaac
      961 gactggagtg acctgatccg actgatcgat aagatcaata acagcggaac agcctttcat
     1021 gattcactgg ccgaagtgct gaacacctgg cctttcttcc gccattgggc tgccgacaac
     1081 ctcttcgcca atctggattc ctacatcggc tccggtcaca attatttcat ttatcacgaa
     1141 agcgtttccg atcagtttga atggattcac tgggacgtca acgaagcgtt cgggaattt
//
LOCUS       DAOTON010000904         1197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_94652_length_1197_cov_3.041156, whole genome shotgun sequence.
ACCESSION   DAOTON010000904 DAOTON010000000
VERSION     DAOTON010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94652_length_1197_cov_3.041156"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="P5292_13795"
     CDS             <1..379
                     /locus_tag="P5292_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HRS40248.1"
                     /translation="GMTTSDIDYINVHGTSTPIGDPQEIKAIQTIFGEDAYKLNISST
                     KSMTGHLLGAAGAIEAIASILSVVNDMVPPTINHFTDDPAFDPKLNLTFNKAQKRTVR
                     AALSNTFGFGGHNASVIFRKYEG"
     gene            400..1122
                     /gene="rnc"
                     /locus_tag="P5292_13800"
     CDS             400..1122
                     /gene="rnc"
                     /locus_tag="P5292_13800"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRS40249.1"
                     /translation="MSFLSGIRHLFSKDKSFVRALRNLLGFTPRNLSLYHLAFKHRST
                     AEEHPSGIKMSNERLEYLGDAVLGAIIAEHLFKRFPFREEGFLTEMRSRLVNREQLNK
                     LAVKIGVDRFMQNSIDPGAKNRSAYGDAFEALIGAVYLDQGYNVTRQLVLNRIFRHHI
                     DVDVVEQTDSNFKSKLINWCQKEKRTVDFELVEEVENGGKRLLRVRVLVNGEEVAQGE
                     DFSKKRAEQIAAEKAIGILGIS"
BASE COUNT          295 a          354 c          315 g          233 t
ORIGIN      
        1 cggcatgacg acctccgaca tcgactacat caacgttcac ggcacgtcga ctccgatcgg
       61 cgatccgcag gaaatcaagg ccatccagac catcttcggc gaggatgcgt acaagctgaa
      121 catcagctcg acgaaatcga tgaccggtca cctgttgggt gcagccggcg ccattgaagc
      181 catcgcgtcg atcctttccg tcgtgaacga tatggttccg ccgaccatca accatttcac
      241 cgacgacccg gccttcgatc cgaaactgaa cctgaccttc aacaaggcgc aaaagcgcac
      301 cgttcgcgcg gccttgagca acaccttcgg ttttggcggg cacaatgctt cggtcatctt
      361 ccgcaagtac gaaggctgag cacagcatcg tccaaccgat tgagtttcct ctccggcata
      421 cggcacctct tttccaagga caaatcgttc gtccgcgccc tgcgcaacct cctggggttt
      481 acgccgcgta acctttcctt ataccatctt gcgttcaagc accggtccac tgccgaagaa
      541 catccgagcg gcatcaagat gagcaacgaa cgcctcgagt atctgggaga cgcggtatta
      601 ggagccatca tcgccgaaca cctcttcaaa cgattcccct ttcgagaaga aggctttctc
      661 acggagatgc gcagccggct cgtcaaccgc gaacaattga acaagctggc ggtaaagatc
      721 ggtgtcgacc gcttcatgca gaactcgatt gaccccggag ccaagaaccg ttcggcttat
      781 ggcgacgcct tcgaagccct gatcggagcc gtttacctgg accagggata caacgtcacc
      841 cggcagctcg tcctcaaccg catcttccga catcacatcg acgttgatgt cgttgaacaa
      901 accgacagca atttcaagag caagctcatc aactggtgcc agaaggagaa gcgcacggtc
      961 gactttgaat tggtggaaga agtggaaaat ggcgggaaac gcctgcttcg cgtccgggta
     1021 ctggtgaacg gtgaagaagt ggcgcagggc gaagactttt cgaaaaagcg ggctgaacag
     1081 atcgcggccg aaaaagcgat cggtatcctg ggtatctcct gaagtcagga agattccggc
     1141 ggggcggacg accgtcgcat aatcctccgt aatttcacac gggatgaaaa aagtcgt
//
LOCUS       DAOTON010000905         1196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_94766_length_1196_cov_3.392638, whole genome shotgun sequence.
ACCESSION   DAOTON010000905 DAOTON010000000
VERSION     DAOTON010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94766_length_1196_cov_3.392638"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="P5292_13805"
     CDS             <1..343
                     /locus_tag="P5292_13805"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRS40250.1"
                     /translation="KEDEFQLYQRLFHIINPHLPKPDLLVYLHKDVPKLKENIRNRGR
                     DYEQNIQDDYLGNLEKGYWDFFKQQDGMRIVVLDTNEVDFVRNMNDYNRILEILQQDF
                     PVGLHRILPKG"
     gene            455..>1196
                     /locus_tag="P5292_13810"
     CDS             455..>1196
                     /locus_tag="P5292_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRS40251.1"
                     /translation="MRFFYTLLAGLVMYAGTVSAQTSITQDSVRVDIKGDKMPRSNEF
                     RTWDIGAHIGFTYPNTDIAASDLTDDKVINKLGFGFNVTKFFTHSFALQASFIHAKLS
                     GIDNNKPYRYETTVNYDISLNALIQIGNIAFLKKTPNLGIYGYMGVGVMNVDPQVSLD
                     DGKTYLNGIYSQYQQPYDTMDYSNTTQLTIPFGVGVKYRIARPFSLSLEYTLRTTNFD
                     KLDGWYKLLSEDDAYSYFNVGLTYHLGAK"
BASE COUNT          298 a          333 c          306 g          259 t
ORIGIN      
        1 gaaggaagac gagttccagt tgtaccaacg gctctttcac atcatcaatc cgcacctgcc
       61 caagccggat ctgttggtct atctgcacaa ggacgtaccc aagctgaagg agaacatccg
      121 gaaccgcggg cgcgactatg agcagaacat ccaggacgat tatctgggca accttgaaaa
      181 gggctactgg gactttttca agcagcagga cggtatgcgg atcgtggttt tagacacgaa
      241 tgaggtggat tttgtgcgta atatgaacga ttacaaccgc atcctcgaga ttctccagca
      301 ggactttccg gtgggactgc accgaatact cccaaagggc tgaaaaccgc attgggtggg
      361 catttgcgcc ttgtcccatt ccgggaagtt gctaaatttg cgaaccttgt aacgggtaag
      421 gcggtataag tcagacccga taaactatca ttccatgaga tttttctaca cgctactggc
      481 cgggctggtc atgtatgccg gaaccgtttc cgcgcagacc agcatcacgc aggacagtgt
      541 ccgcgttgat atcaaaggcg acaagatgcc gcggtcgaac gagtttcgta cctgggacat
      601 cggcgcgcac atcggattca cctatccgaa taccgatatc gccgccagcg atctgacgga
      661 cgacaaagtg atcaacaagc tcggcttcgg tttcaatgtc acgaagttct tcacgcattc
      721 gttcgcgttg caagcctcct tcattcacgc caagctgtcc ggtatcgaca ataacaagcc
      781 gtaccgctac gagaccacgg tcaactacga catttcgctg aacgccctga tccagatcgg
      841 aaacatcgcg ttcctgaaga agaccccgaa cctgggcatc tacggttaca tgggcgtagg
      901 cgtcatgaac gtcgatccgc aggtttcact tgacgacggc aagacgtacc tcaacggtat
      961 ttacagccag taccagcagc cgtacgatac gatggattac tccaacacca cgcagctgac
     1021 gattccgttc ggggtaggtg tgaagtaccg catcgcccgt ccgttctcgc tgagcctgga
     1081 gtacacgctc cgcacgacga acttcgacaa actcgacggc tggtataagc tgctttcgga
     1141 agacgacgcc tattcctatt tcaacgtggg acttacctac caccttggcg cgaaag
//
LOCUS       DAOTON010000906         1194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_95088_length_1194_cov_1.962248, whole genome shotgun sequence.
ACCESSION   DAOTON010000906 DAOTON010000000
VERSION     DAOTON010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95088_length_1194_cov_1.962248"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..>1194
                     /locus_tag="P5292_13815"
     CDS             202..>1194
                     /locus_tag="P5292_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40252.1"
                     /translation="MVQLLKRIFPLVFGLVLGAGLTANAQTSNFRSLYLKDVDVWCGN
                     TTTENTILNYCSGNGFNYIIFYDLGSFDFSSTTKKNQLAAFMSRARQNYGITQFGASG
                     EVYSFFRDYIIPYNNGRTSSAEKFEVFNFEFEWWVSSSVASLYCSKYLTPNGYSCDSA
                     GAWRFSWNEFKKIDSAAAANGRISEYYLGWPTTGHMQQVVSRTDRLLLHAYRPTDVDV
                     YSYSRNRLIDAASTSATVTILPIFSSEPSFMGPWLASNPVTKPYTTYSNAYIAETGSW
                     KSRISLQGYTWFHYGYMPATTSAVATITTSGPTTFCSGGSVTLTANSGSAYLWSP"
BASE COUNT          270 a          348 c          296 g          280 t
ORIGIN      
        1 tggaggttag gtgaaccggg agtcaacatc gttaatatcc atttttgcct cagaatcacg
       61 aggataaacg ctgattatct tcgctcccgc tgaattcagg aggtccgggc attgcgacta
      121 acccggatga gtgatgaccg caaggtcagt ggttcggatc aacggaatcg gcaaacacac
      181 acactcagga aaatcaacaa catggttcaa ttgctgaagc gcatctttcc cctggtattc
      241 gggctggtac tgggagcagg tttgacagcc aatgcacaaa cgagcaactt ccggtcgctg
      301 tacctgaagg atgtagacgt ttggtgcggc aacaccacga cggagaatac catcctcaat
      361 tattgttcgg ggaatggttt caactacatc atcttctacg atctggggtc gttcgacttc
      421 agcagcacta ccaaaaagaa ccaactcgcc gctttcatga gccgtgcccg tcagaactac
      481 gggatcacgc aattcggcgc atcgggggag gtttattctt tcttccggga ttacatcatt
      541 ccctataaca acggccgtac ctccagcgcg gagaaattcg aagtattcaa tttcgaattc
      601 gagtggtggg tatcgtccag cgtagccagc ctctattgca gcaagtatct caccccgaac
      661 ggttactcct gtgattccgc cggtgcctgg cgtttttcct ggaacgagtt caagaaaatc
      721 gattcggcag ccgcagctaa cggccgcatt tccgagtact acctgggctg gcccacgacc
      781 ggacatatgc agcaggtggt aagccgcacc gaccggttgt tgctccacgc ttaccgtccg
      841 acggatgtgg acgtttactc ttattcgcgc aaccgcctga tcgacgccgc gtcgacttcc
      901 gcaaccgtca ccatcctccc gatcttttct tcggaaccct ccttcatggg accgtggctt
      961 gcttccaacc cggtaaccaa gccgtacacg acctactcca atgcctacat tgccgaaacc
     1021 ggttcctgga aatcgcggat cagcctgcag ggttatacct ggttccatta cggttatatg
     1081 cccgccacca cgagcgcggt agctaccatt acgaccagtg gtccgaccac cttctgttcc
     1141 ggcggctcgg ttacactgac ggccaattcg ggatccgctt atctctggag cccg
//
LOCUS       DAOTON010000907         1192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_95286_length_1192_cov_3.423043, whole genome shotgun sequence.
ACCESSION   DAOTON010000907 DAOTON010000000
VERSION     DAOTON010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95286_length_1192_cov_3.423043"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="P5292_13820"
     CDS             <1..682
                     /locus_tag="P5292_13820"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRS40253.1"
                     /translation="TQRLFEPAAHSSRGDWTELMSTSPYQLRPDDKVSVSVWDHEELS
                     VGSIYGIYNSNEVYGKWLLVDAEGNLLLPKTGKLKVGGMTIAAATDAVVREYSRLIPQ
                     PVVTLKVLNREVTVLGEVKSPGPITLEKERNSLIEILGRAGGPDYYANAGQVQLLRMK
                     GQQRIEYVVDLTKLSTVEQESLWVQAGDILYLPPQKRKSLQKESASLVPFAAILSSLA
                     VVLTVISK"
     gene            685..>1192
                     /locus_tag="P5292_13825"
     CDS             685..>1192
                     /locus_tag="P5292_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40254.1"
                     /translation="MNDPQQFKRLLLPALRGLPLILVSVIVAVLLTSRFLHYAVPRYE
                     STALLKLDEKANGVSDNNLFKDFDFFTANSKIITEEQVLQSPVLIEKALRLVDFGTSY
                     YRIGDIRQSELYRQTPFFVQCSLRDSSLLDHRISVQVLDSTRYLMSYNLAMKTVKDSG
                     RFDAPLFLP"
BASE COUNT          262 a          329 c          339 g          262 t
ORIGIN      
        1 gacgcagcga cttttcgaac cggccgcgca cagcagccgc ggcgattgga ctgaactgat
       61 gtcgacgtcg ccctatcaac tgcggcccga cgacaaggtg agcgtgagtg tctgggacca
      121 tgaggagttg agtgtcggtt ccatctacgg tatttacaat tccaatgaag tgtacggcaa
      181 gtggttgctg gtcgatgccg aaggcaacct gttgctcccc aagaccggca agctgaaggt
      241 cggcggaatg accatcgcgg cggctaccga tgcggtcgtg cgcgaatatt cccgactgat
      301 tccgcaaccg gttgttacgt tgaaagtcct gaatcgggaa gtcaccgtac tgggagaagt
      361 caaatcgccc ggaccgatca cgctggaaaa ggaacgtaat tcactcatcg agatcctggg
      421 ccgcgcaggc ggtccggatt attatgccaa cgccggacag gtccagttgc tgcgtatgaa
      481 aggtcagcaa cgcatcgagt atgtggttga tctgaccaaa ctcagcacgg tggagcagga
      541 gagcctctgg gtacaggcgg gcgatattct ctatctgccg ccacagaagc ggaagagtct
      601 gcagaaggaa tcggccagcc tggtaccgtt tgccgcgatc ctctcgagtc tcgccgtcgt
      661 gttaaccgtt atcagcaaat aaccatgaac gatccgcaac aattcaaacg actgctgttg
      721 cccgccctgc gcgggcttcc gctcatcttg gtcagcgtga tcgtagcggt gctgctgact
      781 tcgcggttcc tgcactatgc cgtaccgcgt tacgaaagca ccgcgcttct gaaactcgac
      841 gagaaggcga atggtgtttc cgataacaac ctgttcaagg atttcgactt cttcaccgcg
      901 aatagtaaga tcattaccga ggagcaggtc ctgcaatcgc cggtcctgat cgagaaggct
      961 ctccgcctgg tcgatttcgg cacctcgtac taccgcatcg gtgacattcg tcagagtgaa
     1021 ttgtatcggc aaacgccgtt tttcgtacag tgcagcctgc gcgacagcag tctgctcgac
     1081 catcgcattt ccgtgcaggt attggatagc acccgatatc tgatgagcta taacctggcg
     1141 atgaagaccg tgaaggactc cggccgtttc gatgcgccct tgtttcttcc gg
//
LOCUS       DAOTON010000908         1186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_96037_length_1186_cov_2.437666, whole genome shotgun sequence.
ACCESSION   DAOTON010000908 DAOTON010000000
VERSION     DAOTON010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96037_length_1186_cov_2.437666"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..103
                     /gene="rpsQ"
                     /locus_tag="P5292_13830"
     CDS             <1..103
                     /gene="rpsQ"
                     /locus_tag="P5292_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRS40255.1"
                     /translation="NESNVGDLVKITETRPMSKNKCWRLAQILERAK"
     gene            149..517
                     /gene="rplN"
                     /locus_tag="P5292_13835"
     CDS             149..517
                     /gene="rplN"
                     /locus_tag="P5292_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRS40256.1"
                     /translation="MIQQESRCVVADNSGAKEVLCIRVLGGTKKRYASLGDKIVVTVK
                     HALPSGNIKKGTVSKAVVVRTKKEVKRSDGSYIRFDDNAVVLLNAQDELRGTRIFGPV
                     ARELRDKQFMKIVSLAPEVL"
     gene            543..881
                     /gene="rplX"
                     /locus_tag="P5292_13840"
     CDS             543..881
                     /gene="rplX"
                     /locus_tag="P5292_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRS40257.1"
                     /translation="MERKNNKQAKLNVRKGDTVKVIAGDSKGAQGRVLEVLVSERRVL
                     VEGVNLVTKHTKPNAKNTQGGIVKQEAPIHISNVMVVDGKGNTTRVGRREEGGKLVRY
                     SKKSGEVIKK"
     gene            878..>1186
                     /gene="rplE"
                     /locus_tag="P5292_13845"
     CDS             878..>1186
                     /gene="rplE"
                     /locus_tag="P5292_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRS40258.1"
                     /translation="MSYVPRLKDKYRNDIVPALMKKFNYDSVMQVPRLLKISVNQGVG
                     DAVSDKKLIDSAVAEMSMITGQKAVATKSRKDIANFKLRAGVPVGVRVTLRETNMYEF
                     L"
BASE COUNT          323 a          302 c          353 g          208 t
ORIGIN      
        1 gaacgaatcg aacgtcggcg acctggtgaa gatcaccgag acccgtccga tgagtaagaa
       61 caaatgctgg cgcctcgccc agatcctgga gcgggctaag taatcccgta ggacagcaga
      121 atcattaatc agttaaccaa ggaaagtcat gatccagcag gaatcccggt gcgtagtagc
      181 cgacaacagc ggtgccaagg aagtactctg cattcgcgtg ttgggcggca ccaagaagcg
      241 ctatgcttcg ttgggcgaca agatcgtcgt cacggtaaag cacgcgctgc cctccggtaa
      301 tatcaaaaag ggtacggttt cgaaagccgt cgtggtccgc accaagaaag aagtgaagcg
      361 ttcggacggg tcgtacatcc gtttcgatga caacgccgtc gtgttgctga atgcacagga
      421 cgaattgcgc ggaacccgca tcttcggccc ggtagcacgt gaacttcgtg ataagcagtt
      481 catgaagatc gtctccctgg ctccggaagt actctgatct caaccgtttt agacaagaaa
      541 cgatggaaag aaaaaacaac aagcaagcga agctgaacgt ccgcaaaggg gacaccgtga
      601 aagtgattgc cggtgactcc aaaggcgcac agggacgcgt gctggaggtg ctcgtgagcg
      661 agcgccgcgt gctggtcgaa ggtgtcaacc tggtgaccaa gcacaccaag cccaacgcca
      721 agaacaccca gggcggcatt gtgaagcagg aagcgccgat tcacatctcg aatgtgatgg
      781 tcgtcgacgg caagggtaac acgacccgcg taggacgccg tgaagagggt ggaaaattag
      841 ttcgatactc taaaaagtcc ggggaggtaa tcaagaaatg agttacgtac cgcgcctgaa
      901 agacaagtac cggaatgaca tcgttccggc actgatgaag aaattcaatt acgacagcgt
      961 gatgcaggtt ccgcgcctgc tgaagatcag cgtgaatcag ggtgtcggcg acgccgtgag
     1021 cgacaagaag ctgatcgact ccgccgtagc cgagatgtcg atgatcacgg gtcagaaagc
     1081 cgtagcgacc aaatcgcgca aggacattgc gaacttcaag ctgcgtgccg gagtaccggt
     1141 cggcgtgcgc gtcacccttc gggagacgaa catgtacgaa ttcctg
//
LOCUS       DAOTON010000909         1182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_96544_length_1182_cov_2.551020, whole genome shotgun sequence.
ACCESSION   DAOTON010000909 DAOTON010000000
VERSION     DAOTON010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96544_length_1182_cov_2.551020"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="P5292_13850"
     CDS             <1..886
                     /locus_tag="P5292_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HRS40259.1"
                     /translation="AAAASPEGTSAVVSNPVTMTVSDGLPTISSFTPTSGTSGTTVTI
                     NGTNFVGVSAVKFNGTNASFTVVNATQITATVPAGATTGLISVTNNCGTGNSATNFTV
                     GAATANLSLQVLLQGMYIGSGQMIAAINPSGNPNACDTITVSLAAATSPYAILYSNKD
                     TINLSGLGVFTFPGAVIGSSYYIVVRHRNSLETWSAAPVSFSSSNISYNFQNSAAQAY
                     GSNQASLGGGKFGLHSGDTNQDDLIEASDYSAVENAVQSFLFGYVATDLNGDQLVEAS
                     DYSLVENNSLLFLFTAAP"
     gene            1015..1088
                     /locus_tag="P5292_13855"
     tRNA            1015..1088
                     /locus_tag="P5292_13855"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1049..1051,aa:Ala,seq:ggc)
BASE COUNT          280 a          343 c          295 g          264 t
ORIGIN      
        1 ggctgctgct gcatcgccgg aagggaccag cgcggtggtg tccaatccgg taaccatgac
       61 ggtcagcgac ggcctaccga cgatcagctc cttcacaccg acgagcggga catccggcac
      121 cactgttacg atcaacggca cgaacttcgt cggggtcagt gcggtgaagt tcaacggaac
      181 caatgcctcg ttcacggtgg tgaacgctac ccagatcacc gctacggttc cggccggcgc
      241 caccacagga ctgatcagtg tcaccaacaa ctgtggaacc ggaaacagtg caaccaactt
      301 caccgtaggt gcagccactg cgaacctgag tcttcaggtc ttgttacagg gcatgtatat
      361 cggcagcgga caaatgatcg cggccatcaa cccatccgga aatccgaatg cctgcgatac
      421 gatcaccgta tccctggctg ccgccacatc accctatgcc atcctgtatt cgaacaagga
      481 taccatcaac ctctccggac tcggcgtctt tacgttcccg ggcgcagtca tcggcagcag
      541 ttattacatc gtcgttcgcc accgcaatag tctcgaaacc tggagtgcag caccggtatc
      601 cttcagcagc tcgaacattt cgtacaattt ccaaaactcc gccgcgcaag cctatggcag
      661 caaccaggcc agcctgggag gtggcaagtt cggactccat tcaggagata cgaatcaaga
      721 tgacctcatt gaagcttccg actattccgc ggtggagaac gctgttcagt ccttcctctt
      781 cggctatgta gccaccgatc tgaacgggga tcaattggta gaggcatccg attattcgct
      841 ggtcgagaac aacagcttgc tgttcctgtt cactgccgca ccctaagcgc caatatctga
      901 acgttctgaa aatcaagggg gaaaatctct gcagcagccg ataccttgat ttcagttgaa
      961 aattcgtata tttgcaaccg cctaaacagc aaattctgct ctttaggcgg acacggggga
     1021 ttagctcagc tggctagagc gtttgcatgg catgcaaaag gtcatcggtt cgactccgat
     1081 attctccact caaaaggctg accgtttggt cggccttttt ttattggccc tgtcacgtgt
     1141 acggcattga ttaaattcgg cggatcatga gcatcagtta ct
//
LOCUS       DAOTON010000910         1180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_96815_length_1180_cov_3.088000, whole genome shotgun sequence.
ACCESSION   DAOTON010000910 DAOTON010000000
VERSION     DAOTON010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96815_length_1180_cov_3.088000"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..>1180
                     /locus_tag="P5292_13860"
     CDS             12..>1180
                     /locus_tag="P5292_13860"
                     /inference="COORDINATES: protein motif:HMM:NF014110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40260.1"
                     /translation="MVHFWNARDGWAMGDPNPSEFELYTTADGGDTWTPTDPANIPDP
                     LAGEYGIVGHYNVIGDNIWFDTNKGRVYYSTDRGQTWNVGTTAIAVPANGAIDICFWS
                     PTNGLARIYNQTTGANTVKFTTDGGATWSAGTITGTFFGSDVKHIPGTTSKLISTGAA
                     TGFVGSSFSDDGGLTWNTIETLAQRTALGVVDSMHMWTGGFTVSPTEDGIFKWATLEP
                     VLCNDPTINPGTATSDVTELCEGDTATFTATGVLAPNEGDFSGVSWIISSADITGISD
                     PLSDPSLVATYTFSFPAPATSIRLYINDGTLIDGSLVPYGLYYWTPVVFANATNPGST
                     APVFLSDLTLDPLCTYTGNSVPVTVYDPNDPACLGTGVAELNSNDFSVLANQSDA"
BASE COUNT          232 a          396 c          292 g          260 t
ORIGIN      
        1 ccttcccgaa cgtggtgcat ttctggaacg cccgtgacgg ctgggccatg ggtgatccga
       61 acccttccga gttcgaactc tacaccaccg ctgacggagg cgacacctgg accccgaccg
      121 atcctgccaa catccccgac ccgcttgccg gtgagtatgg tatcgttggt cactacaatg
      181 tgatcggtga caacatctgg ttcgacacca acaaaggccg tgtttactat tctacggacc
      241 gcggtcagac ctggaacgta ggcacgacgg caatcgctgt tcctgcaaac ggtgccatcg
      301 acatctgctt ctggtcaccg accaacggtc ttgcccgtat ctacaaccaa accaccggtg
      361 ccaataccgt taaattcacc accgacggtg gtgctacctg gtcggccggc acgatcaccg
      421 gaaccttctt cggttctgat gtgaagcaca ttcccggcac cacgtccaaa ctgatctcca
      481 ccggcgcagc taccggcttc gttggttcct ccttcagcga cgacggtggc ctcacctgga
      541 ataccattga aacgcttgcg cagcgcaccg cacttggtgt tgtcgactcc atgcacatgt
      601 ggaccggcgg ctttacggtc agcccgaccg aagacggcat tttcaagtgg gctacccttg
      661 agccagttct ttgcaacgat ccgaccatca atcccggaac cgcgacttcc gatgtgaccg
      721 aactgtgcga aggcgacacc gctaccttca ccgcgaccgg agtattggct ccgaacgagg
      781 gtgatttctc cggtgtgagc tggattatct cctctgccga catcaccggc atttcggacc
      841 cgctgagcga cccgagcctg gttgcgacct acacgttctc tttccctgct cctgccactt
      901 cgatccgtct ctacatcaac gatggtaccc tgatcgatgg ctccctggtt ccgtatggcc
      961 tgtactattg gaccccggtc gtattcgcca atgctaccaa ccccggttcg actgctccgg
     1021 tattcctgag cgacctcacc ctggatccgc tgtgtaccta caccggcaac tcggtaccgg
     1081 ttaccgttta cgacccgaac gatccggcct gtctgggtac cggtgtcgcc gaactcaaca
     1141 gcaacgactt cagcgttctt gctaaccagt ccgacgccaa
//
LOCUS       DAOTON010000911         1177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_97182_length_1177_cov_2.677362, whole genome shotgun sequence.
ACCESSION   DAOTON010000911 DAOTON010000000
VERSION     DAOTON010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97182_length_1177_cov_2.677362"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="P5292_13865"
     CDS             <1..575
                     /locus_tag="P5292_13865"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRS40261.1"
                     /translation="GARVYSDCVIGKDCTLHTGVVVGADGFGFTPNRENQYQKVAQIG
                     NVIIEDHVEIGANSTVDRATLGSTIIRKGAKLDNLIQIAHNVEIGENTVIAAQTGVAG
                     STKIGRDCMIGGQVGIVGHITIADQVKIAAQSGIGSSIVTPGEIVQGSPAFNIGDYKR
                     TYVVFRKLPQLERRIQELEQMLVDLKQLMG"
     gene            682..>1177
                     /locus_tag="P5292_13870"
     CDS             682..>1177
                     /locus_tag="P5292_13870"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRS40262.1"
                     /translation="MPENQIIAEATPKTAVAKPAAGVPVFQTTIKDSITISGVGLHTG
                     ASVNLTFRPAPEHHGYKFCRVDLPGKPVIDADVDFVGDTERGTTLVKDGARVSTIEHV
                     LAALVGMEIDNALMEVDGPEIPILDGSSLPFIELLERVGIVEQSAERKYFVLQENLTY
                     EDPSR"
BASE COUNT          273 a          283 c          341 g          280 t
ORIGIN      
        1 ccggtgcccg tgtctattcc gactgtgtaa tcgggaagga ttgtacgttg catacaggag
       61 tggtcgtagg agccgatggt ttcggattta caccgaaccg ggaaaaccaa taccagaaag
      121 ttgcccagat cggcaatgtg atcatcgaag accacgtgga gatcggggct aacagtacgg
      181 tcgatcgtgc gaccctggga tcaacgatca tccgcaaggg cgcaaagttg gataacctta
      241 ttcagatcgc ccacaatgtg gagataggag agaatactgt tattgctgca caaaccggag
      301 ttgccgggtc aaccaagatt ggacgcgatt gtatgatcgg tggccaggtt gggatcgttg
      361 gacatatcac aatcgcggat caggtcaaga tcgctgccca gtcgggtatc ggttccagta
      421 tcgtaacccc gggtgagatt gttcagggtt ctcccgcttt taacatcggc gactacaagc
      481 gaacctatgt ggtcttccga aagcttcctc agttggaacg caggatccag gagttggagc
      541 aaatgctggt ggatctaaaa cagctgatgg gttaagctgt ttttggcccg aattcgggct
      601 ttttactgtg gatttatttt ttaattttgg cgacccccaa ctttgtccgc ctacccggac
      661 atggggccaa tcatttttca gatgcctgaa aatcagatta ttgcagaggc tacgccgaag
      721 actgcagtag ccaagcctgc tgcgggtgtt ccggtttttc aaacgacgat caaagactcc
      781 attaccattt ccggagtggg attgcatacg ggtgcaagcg tcaacctgac atttcgcccg
      841 gctccggagc atcatggcta caagttctgt cgtgttgatc ttcccgggaa accggtcatc
      901 gatgcggatg tcgattttgt cggggatacc gaacgcggga ccacgctcgt caaggacggg
      961 gcccgggtca gtacgatcga acacgtgctt gcagcgctgg tgggaatgga gatcgataac
     1021 gctctcatgg aagttgacgg gccggagatt ccgatcctgg acggaagttc gctcccgttc
     1081 atcgaactgc tggagcgggt cggtattgtg gagcagtcgg ccgaacggaa gtactttgta
     1141 ttgcaggaga acctgaccta cgaagaccct tcccgca
//
LOCUS       DAOTON010000912         1177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_97220_length_1177_cov_2.263815, whole genome shotgun sequence.
ACCESSION   DAOTON010000912 DAOTON010000000
VERSION     DAOTON010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97220_length_1177_cov_2.263815"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1177
                     /locus_tag="P5292_13875"
     CDS             <1..>1177
                     /locus_tag="P5292_13875"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRS40263.1"
                     /translation="TATGLPAGSYSVTVMDANGCISTDNVTITEPSPVQAQASATAAL
                     CRSTATGAVSAQAAGGTGPYTYLWMPGSIAGASASNLNAGTYTVTVSDQNGCTSTATA
                     SVTQPTAVTASTSTQATQCNGSADGSATVSANGGTPGYTYSWFPTGGNNSTTIGVAAG
                     TYTVTVTDDNGCSITSTAVVGQPAAINVSTSSTPASCGSANGSATASVSGGAGSYSYA
                     WSPGGATGANASGLVAGSYTVIVTDANGCTQTANASVSNIGGPTIATAVVQQVSCFGG
                     NDGSATVNVSTGTAPFSFAWSPAGGSGATATGLSAGNFSVTVTDANGCISNDQVTITE
                     PTALALQTTTQPTLCFGSPSGSADVVVAGGTGPYSYAWAPGGASSASAINLSAGNYTV
                     "
BASE COUNT          255 a          371 c          319 g          232 t
ORIGIN      
        1 cgacagccac cggcttgcct gccggaagtt attcggttac cgtaatggat gccaatggtt
       61 gtatctcgac cgacaacgtg accattacgg aaccctcacc ggtacaggct caggccagtg
      121 cgacagcagc attgtgtcgc agcacagcaa ccggcgcggt cagcgcgcaa gctgccggcg
      181 gaaccggacc ctatacctat ctctggatgc cgggcagtat tgcgggcgcc tccgcttcca
      241 atctgaatgc aggaacttat acggttacag tatccgacca aaacggctgt acgtcaactg
      301 ccactgcttc tgtcactcag ccgaccgccg tcaccgcttc gacctcgaca caagccacac
      361 aatgtaacgg cagcgccgat ggtagcgcca cggtttccgc gaatggcggg acaccgggct
      421 acacgtatag ctggttcccg acaggcggca acaatagtac gacgattggc gtcgctgcag
      481 gaacctatac cgtaaccgta accgatgata acggttgttc catcacgtca accgccgtcg
      541 ttggccaacc ggccgcgatc aatgtcagca cttcttcaac tccggcatcc tgcggatcgg
      601 ctaacggatc ggcaactgcc agcgtgagcg gcggcgctgg ctcttattct tacgcctgga
      661 gccctggcgg tgcaaccgga gcaaatgcaa gcggacttgt agcaggcagc tacaccgtca
      721 tcgtcaccga cgccaacggg tgtacccaga ctgcgaatgc cagcgtttcc aatatcggcg
      781 gcccgaccat tgcaactgct gttgtgcaac aagtctcctg cttcggcgga aacgacggta
      841 gcgcgaccgt gaatgtcagc accggcacag cgccattcag ctttgcatgg tcccctgccg
      901 ggggttcggg cgcaactgca acgggacttt ctgccggcaa tttcagtgtt accgtaacgg
      961 atgcgaatgg ttgcatttcc aatgaccagg taacgatcac cgaaccgacc gccctagcgc
     1021 tgcagactac aacgcaaccc accttatgct tcggatcgcc atccggatca gcggatgtcg
     1081 tagttgccgg tggaaccgga ccctatagtt atgcctgggc tccaggtggt gcttcatccg
     1141 cttccgccat caatctcagc gccggaaatt atacagt
//
LOCUS       DAOTON010000913         1176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_97374_length_1176_cov_2.120428, whole genome shotgun sequence.
ACCESSION   DAOTON010000913 DAOTON010000000
VERSION     DAOTON010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97374_length_1176_cov_2.120428"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..>1176
                     /locus_tag="P5292_13880"
     CDS             109..>1176
                     /locus_tag="P5292_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40264.1"
                     /translation="MEWDQFLFKRISELVQWVRRPAADPQRQAAAVELETLKPRLTAL
                     ARLLSGRAVNIHPAEREGGWRGDQWLLPSRYDRSATQTGNVDFFLFRIFFLYGQERLR
                     HAFRGEEANSILQASACALLNADAVLAFLSNEFPGFDELHQRVKEDAMAGHDASDPAE
                     PWLYGRWYRLDDDSWCKLDANADAASKNTVAVEKDSKDYSERQAPAREETELLIVDSE
                     EQENYTLTHNFEKIETLDTFSGRWRDFDGSDDMDEHGEALQELDLRQLVRVDSPVHSV
                     YRTEFIQSLGLIETADAVHAGFHRCYDEWNFQQHGYRKGYCRVYPTFLKESLSGYAAT
                     TLSKHAGLARSMRLQAERYLTE"
BASE COUNT          268 a          286 c          367 g          255 t
ORIGIN      
        1 cggaatgccg ccgcggcttt cctgtacggt agcgatcgca gaaacgttga cggacgaccc
       61 ggaagtgcgt cagtcgctgg ttgacctgat caacatgacc ttttaacaat ggagtgggat
      121 cagtttctgt tcaaacggat ctccgaactc gtacaatggg tcagacgtcc cgcggcggat
      181 ccgcaacggc aagcggcggc ggtggagttg gaaaccctaa aaccgcgact gacagccctg
      241 gctcgtctct tgtcggggcg cgcggtgaac atacatcccg ccgagcgcga aggtggctgg
      301 cgaggagatc agtggttgtt gccgtcgcgg tatgaccgtt cggcaacgca aaccggcaat
      361 gttgattttt ttctattcag gatcttcttc ctgtatggtc aagagcgact gcgacatgct
      421 tttcgtggag aggaggcgaa tagtatacta caggcgagcg cgtgcgcgct gctgaacgcg
      481 gacgcggtgc tggcttttct gtcgaacgaa tttccgggat ttgacgaatt gcatcaacgc
      541 gtgaaagaag acgcgatggc cggacacgat gcgtcggatc cggccgaacc ctggttgtat
      601 ggccgttggt atcgactcga tgacgacagc tggtgcaagc tcgacgcaaa tgctgatgct
      661 gcatcgaaaa ataccgtggc agtagagaaa gattcgaagg attattcaga aaggcaggcg
      721 ccggcgcggg aggaaaccga gctgttgatc gtcgattcgg aggaacaaga gaactatacg
      781 ctcacccaca atttcgaaaa gatcgagacg ctggatacgt tctccggtcg gtggcgcgat
      841 tttgacggaa gcgacgacat ggatgagcat ggcgaagcat tgcaggaatt ggacctccgg
      901 caactggtac gcgtggatag cccggtgcat tcggtgtatc ggacggaatt cattcaatcg
      961 ctgggcctga tcgaaaccgc tgacgctgtt catgccggat ttcatcggtg ttacgacgaa
     1021 tggaatttcc agcaacatgg ttatcgtaag ggttattgcc gggtctatcc gaccttccta
     1081 aaagagtcat tgtcgggtta tgcggcgacg actttgagca aacacgccgg tttggcgcgt
     1141 tcgatgcggc tgcaggcaga gcgatacctg accgag
//
LOCUS       DAOTON010000914         1173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_97728_length_1173_cov_3.029517, whole genome shotgun sequence.
ACCESSION   DAOTON010000914 DAOTON010000000
VERSION     DAOTON010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97728_length_1173_cov_3.029517"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="P5292_13885"
     CDS             <1..248
                     /locus_tag="P5292_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40265.1"
                     /translation="GGGHTTSPMNTKYAVSGADFSQKSLTAGFGVRDKNVFLDFGYAY
                     TETKGYLQPYTLNFLEVAGVRETMVRSNFTMTFGVKF"
     gene            complement(416..>1173)
                     /locus_tag="P5292_13890"
     CDS             complement(416..>1173)
                     /locus_tag="P5292_13890"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRS40266.1"
                     /translation="STVSIRMRAGRNDDALQLLTRHYPDTFTYAFPFLTYKRGLARLR
                     KLDLGAESDFRAFLNQYKGMNYVRAAWQKLAWIQLLKGDPDGYRQVIQTCRTTGDQLV
                     DEDKEAFSEALGGDPPNKLLLRARLLFDGGYYDEAQDVIAGKPIDSFPRYRDQLEVTY
                     RLARILQRQGQEAKAIEYYETTLKNGSSARYYFAANSALSLGNLYEEKGDLQRALTYY
                     QRCLALDHHEYQNSIDQKAQAGLDRIAEALEKQ"
BASE COUNT          279 a          311 c          309 g          274 t
ORIGIN      
        1 ccggcggagg acacaccacc agtccgatga acaccaagta tgccgtaagc ggcgcggact
       61 tttcgcagaa gtcgctcacc gccggattcg gtgtacggga taagaatgta ttcctcgact
      121 tcggttacgc ctacaccgaa acgaaaggtt atctgcagcc ctacacgctt aacttcctcg
      181 aagtagccgg cgtacgggaa acaatggtac ggtcgaactt caccatgacg tttggggtga
      241 agttctaagg gtaactagtt ccgagtttcg ggtttcgagt tccgggtttg ataggggtac
      301 gtaggacgtg ggacgaaaac acttttaata ggaagccggg atcaataaag tactcagcac
      361 tttcaaccaa aaactttcaa ctttcaacca acccgaaacc cggaactaaa aaaccctatt
      421 gtttctccag cgcctccgcg atccgatcca gccctgcctg ggctttttga tcaatgctgt
      481 tttggtactc gtggtgatcc agggcgaggc aacgctgata ataagttagg gcgcgctgca
      541 ggtcaccttt ctcttcgtag agattaccga gagaaagcgc gctgttcgcg gcaaaataat
      601 aacgagccga agaaccgttc ttgagcgtgg tctcgtaata ctcgatcgct tttgcttctt
      661 gtccctgtcg ttgtaagata cgagccagac gatatgtcac ttccaactgg tcacgatacc
      721 gcgggaaact atcgatcggc ttaccggcaa tcacgtcttg cgcctcgtca taataaccgc
      781 cgtcgaagag caggcgggca cggagcaaca acttgttggg cgggtctccg ccgagcgcct
      841 cactgaaggc ttctttatct tcatctacca actggtcgcc cgtggttctg caggtttgga
      901 tcacctgccg ataaccgtcc ggatctcctt tgagcaactg tatccaagcc agcttctgcc
      961 aggcggcgcg aacgtagttc atgcctttgt actggttcag aaaagcacgg aaatcggatt
     1021 ccgctcccag gtcaagtttg cgcaagcggg ccaggcctcg cttataggtc agaaacggga
     1081 acgcataggt aaaggtgtca ggataatgcc gggtaagcaa ttgcagcgcg tcatcgttcc
     1141 ttccggcgcg catgcggatg cttacggtag aat
//
LOCUS       DAOTON010000915         1171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_97983_length_1171_cov_3.527778, whole genome shotgun sequence.
ACCESSION   DAOTON010000915 DAOTON010000000
VERSION     DAOTON010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97983_length_1171_cov_3.527778"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="P5292_13895"
     CDS             complement(<1..360)
                     /locus_tag="P5292_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40267.1"
                     /translation="MIHHFTKTSKRYLLNKVAVALMMICSQLLWVNEAKATHSAGSDL
                     TYTWVSGNTYRVTVAFYRDCAGVAAPNSITLNARSSSCSRNQNFTLNLVAGSGQEITF
                     PCRTAQTKCTNSSSSNAG"
     gene            complement(538..>1171)
                     /locus_tag="P5292_13900"
     CDS             complement(538..>1171)
                     /locus_tag="P5292_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRS40268.1"
                     /translation="EGIAVEKGTQEPLAGVLVELLNRKTGKKETAVTGPDGKFMFKLN
                     RETEYSVVGSKDGFFTNTEIVSTVGKVRSENMFVKLKLELEKIIINKPIVLENIYYDL
                     DKWDIRPDAAVELDKLVQTLKDNPGIRIELSSHTDSRADDKYNDVLSQKRAESAVQYL
                     TSRGIDASRMIAKGYGERVLVNGCKNDVKCTEEEHQANRRTEFKVIEMTK"
BASE COUNT          252 a          318 c          281 g          320 t
ORIGIN      
        1 acccgcgttg ctggaagaag aattcgtaca cttggtctga gcggtccggc acggaaaggt
       61 gatctcctgt ccggaacccg ctacgaggtt cagggtaaaa ttctgattac gcgaacagct
      121 cgaagagcgg gcgttcagtg tgatggaatt gggcgcggca acacccgcgc aatcccggta
      181 aaacgcaacg gttacccggt acgtatttcc gctcacccag gtgtaggtca gatcgctccc
      241 ggcagagtgc gtagccttcg cttcgttcac ccacaacagt tgtgagcaaa tcatcatcag
      301 cgccacggct actttgttca agagataccg cttcgaagtt ttggtaaaat gatggatcat
      361 ttcgtctgga attatttcgg gcgcgcggtt ggacacaagc gcctttaagg aagcaatttc
      421 gttgattttc attgccagac tgcctctgct tacacagcct ttttaacacg cttatacaca
      481 aattaaaaag gggctgcgcg atcagcacag cccctttttc agtcgcctgg taccaggtta
      541 ttttgtcatt tcgatcacct tgaattctgt ccggcggttg gcctggtgct cttcttcggt
      601 acacttgaca tcattcttgc aaccgtttac cagcacccgc tctccgtagc ctttcgcgat
      661 catccggctg gcatcgatac cacggctggt caggtattga acggccgatt cagcgcgttt
      721 ctgagagagt acgtcgttgt acttatcatc agcgcgacta tcggtatgcg aactcagttc
      781 gatccggatg ccggggttgt ccttgagcgt ttgtactaat ttatcaagtt caacagccgc
      841 gtcaggacgg atgtcccatt tatccaggtc gtagtagatg ttctccagga caatcggctt
      901 gttgatgatg atcttttcaa gttccaattt gagcttcacg aacatgttct cgctgcggac
      961 tttaccaacg gtgcttacga tctccgtatt ggtgaagaat ccgtccttac taccaacaac
     1021 actgtattcc gtctcccggt tcagcttgaa catgaactta ccgtccggac cggtaacagc
     1081 ggtttccttc ttcccggtct ttcggttcaa caattcaacc aacacaccgg ccagcggttc
     1141 ttgggtaccc ttctctacgg cgataccttc c
//
LOCUS       DAOTON010000916         1166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_98585_length_1166_cov_3.287129, whole genome shotgun sequence.
ACCESSION   DAOTON010000916 DAOTON010000000
VERSION     DAOTON010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98585_length_1166_cov_3.287129"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1166
                     /locus_tag="P5292_13905"
     CDS             <1..>1166
                     /locus_tag="P5292_13905"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRS40269.1"
                     /translation="PFVCLGQQFCFNHGAYDSEGDSLVYQLITPYDRAGTPVTYLAPF
                     TSNQPLSSSPAMSFNPQTGDICMTPTNLEVTVMAVLVKEYRNGVLIGSVERDIQVTVI
                     TCSNIIPTLTGINGTNSFAMTICAGEQTCFDVFSSDVDAGQNTFVTWDYAIPGADFTA
                     HPAPRETGTFCWTPTQADVNVIPYCFTVTVRDDNCPYTGSQIYSFCITVRGLDVDAGP
                     DANVVCNSYTTLTASATGGSGAYTYQWNTGQTTTAITAGAGVYTVTASDGTCSNSDTV
                     NVTPANGAPQAGFSIAQTCTSLAVGFTDQSTIVGGTITSWSWNFGDGGTSAAQSPTHT
                     YAATGTYTVMLIVQTATGCIDTVRQSLLLSNNRPTAAFTTTNVCFGEQANFDDG"
BASE COUNT          232 a          386 c          320 g          228 t
ORIGIN      
        1 tgccgttcgt ctgcctcgga cagcagttct gcttcaatca cggcgcgtac gattcggaag
       61 gcgactcgct cgtgtatcaa ctgatcaccc cctacgaccg ggccggtacg ccggtgactt
      121 acctcgcgcc gtttacttcg aaccagccgc tctcctcgag tccggccatg tcgttcaatc
      181 cgcagaccgg cgatatctgc atgaccccga ccaacctcga ggtgacggtc atggccgtgt
      241 tggtgaaaga gtaccgcaac ggcgtgctga tcggcagcgt ggagcgcgat atccaggtga
      301 cggtcattac ctgtagcaac atcatcccga cgctgaccgg catcaacggt accaacagct
      361 tcgcgatgac gatctgcgcg ggtgaacaaa cctgcttcga tgtgttctcc tccgacgtgg
      421 acgccggcca gaacaccttc gtgacctggg actatgccat ccccggcgca gatttcaccg
      481 ctcaccctgc gccgcgcgag accgggacgt tctgctggac gccgacccag gcggatgtga
      541 acgtgatccc gtattgcttc accgtcacgg tacgcgacga caattgtccg tacaccggtt
      601 cccagatcta ttccttctgc atcaccgtgc gcggactgga cgtggatgcc ggccccgatg
      661 ccaacgtggt ctgtaacagc tataccacac tgaccgcttc cgcaacagga ggcagcggtg
      721 cctataccta tcaatggaat accggccaga cgaccacggc catcaccgcc ggtgccggtg
      781 tgtacaccgt cacagccagc gatggaacct gttcgaacag cgataccgtg aatgtgacac
      841 ccgccaacgg cgcgccgcag gccggcttca gcatcgccca gacgtgtacc agtctggctg
      901 tcggttttac ggaccagtcg acgatcgtgg gtggaaccat cacttcctgg tcgtggaact
      961 tcggcgacgg cggaacatcc gctgcgcaaa gcccgacgca tacctatgcc gcgaccggta
     1021 cttacaccgt catgctgatc gtgcaaacgg ccaccggttg tatcgatacc gtacggcagt
     1081 cgctgctcct gtcgaacaac cgcccgacgg ccgctttcac cactaccaat gtttgttttg
     1141 gcgaacaggc gaatttcgac gacggc
//
LOCUS       DAOTON010000917         1166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_98596_length_1166_cov_2.836184, whole genome shotgun sequence.
ACCESSION   DAOTON010000917 DAOTON010000000
VERSION     DAOTON010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98596_length_1166_cov_2.836184"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="P5292_13910"
     CDS             complement(<1..465)
                     /locus_tag="P5292_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRS40270.1"
                     /translation="MPLINSFISWWMKKRMHQIDLFLKYPIEVQQEWFRRLVDSAKHT
                     EWGQRFDYRSIRTLEDFKSRVPVQNYDSLQPYIQRLMAGEQNILWPSEVKWFAKSSGT
                     TAGKSKFIPVSQEALDECHYKGGKDLLSIYCSNYPDTLIFTGKGLTLGGSHRA"
     gene            complement(492..>1166)
                     /locus_tag="P5292_13915"
     CDS             complement(492..>1166)
                     /locus_tag="P5292_13915"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRS40271.1"
                     /translation="HALCMGDEFQPDMVAGHSLGEFSALVANKVLSFQDGLRLVKTRA
                     FAMQKACELRPSTMAAILNLEDAVVEQICADISASGQIVVAANYNCPGQLVISGTIEG
                     VNEACEKLKAAGAKRALVLPVGGAFHSPLMEPARAELAEGIRTTTFSEPICPIYQNVT
                     AQAVTDTNAIRENLIAQLTAPVRWTQTIQAMIADGATSFVEVGPGKVLQGLVKKINKD
                     MEAVSA"
BASE COUNT          248 a          328 c          304 g          286 t
ORIGIN      
        1 agcccgatgg cttcctccca gggtaagtcc tttgccggta aagatcagcg tgtcaggata
       61 gttcgaacaa tagatactca gcaggtcttt ccctcctttg taatgacact catccagggc
      121 ttcctggcta acgggaatga acttgctctt cccggctgta gttccggaag atttggcgaa
      181 ccacttcact tccgagggcc agaggatatt ctgctcccct gccatcaggc gctgaatgta
      241 gggctgtaaa ctgtcgtagt tctgaaccgg tacccgggat ttaaaatctt ccagggtccg
      301 gatacttcgg taatcaaaac gctgacccca ttcggtatgc tttgcactgt cgaccagccg
      361 ccgaaaccat tcctgctgca cctcgatcgg atacttgagg aacaggtcga tctggtgcat
      421 gcgttttttc atccaccagc tgataaagga attgataagc ggcatcgttc cggtatttaa
      481 tattccgaac gtcaggcgga aacggcttcc atgtctttat tgatcttctt gaccaaacct
      541 tgcagcactt tcccggggcc gacttccacg aaagaagttg cgccatccgc gatcatggcc
      601 tggatggttt gtgtccagcg gaccggggcg gtcaactgcg cgatgaggtt ttcccggatc
      661 gcgttcgtat cggtaacagc ctgtgccgtc acgttttggt aaatcggaca gatcggttca
      721 ctgaacgttg tcgtacggat accttccgcc aactcggctc tggccggctc catcaagggt
      781 gaatggaatg cgcctcctac cggcaacacc agtgctcgct tggctccggc cgctttcagc
      841 ttttcgcagg cttcgttgac accttcgatc gttccggaga tcacgagttg tccgggacag
      901 ttgtagttgg ctgccacgac aatctggccg ctggccgata tatccgcgca gatctgttct
      961 acgaccgcgt cctcaaggtt cagaatggcc gccatggtgg agggacggag ttcacacgcc
     1021 ttctgcatcg cgaaagcacg ggtcttcaca agccgaaggc cgtcctgaaa ggaaagaact
     1081 ttgtttgcca ccagtgccga aaattcgccc aatgaatgac cggctaccat atccggctgg
     1141 aattcatcgc ccatgcaaag cgcatg
//
LOCUS       DAOTON010000918         1165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_98724_length_1165_cov_3.181982, whole genome shotgun sequence.
ACCESSION   DAOTON010000918 DAOTON010000000
VERSION     DAOTON010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98724_length_1165_cov_3.181982"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="P5292_13920"
     CDS             complement(<1..597)
                     /locus_tag="P5292_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40272.1"
                     /translation="MNSILRIALWAGCFFLIANNTIGQSLIRNMSSTDGAVNAIVKND
                     GVYYIGGNFNYVGLMTGGASLVTDTNDYPDMDFPTINGNVLTSVPDGNGGWFIGGTFS
                     YLNNINNTCLAHVRNDKSIDANWAANLSASYCGEVRSLFRAGDTLFVGGSFSSIGSSS
                     RNNLAAIRISTGAVLNWNPNCDGAVNTILVRDTTVYIGG"
     gene            complement(947..>1165)
                     /locus_tag="P5292_13925"
     CDS             complement(947..>1165)
                     /locus_tag="P5292_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40273.1"
                     /translation="ANTIRVRLQAKKAGNVRLEIMDLTGRVVTRMNQYVGQGLTIQHL
                     DATQLPAGTYIIRAEQNGVVGTGKVVKL"
BASE COUNT          305 a          272 c          299 g          289 t
ORIGIN      
        1 tccgccgatg taaacggtcg tgtccctgac taaaatggta ttgacagcgc catcgcaatt
       61 cgggttccag ttcaagactg ccccggtgga gatgcgaatg gcggccagat tgtttctgct
      121 gctgcttcca atgcttgaaa aactgccgcc gacgaaaagg gtatctcctg cacgaaacaa
      181 tgaccgaacc tcgccgcagt aactggcaga taaattggca gcccagttag catcaatgga
      241 tttatcattg cggacatgcg ccaggcaggt attgttaatg ttattcaggt aactgaaagt
      301 gcctccaatg aaccaaccac catttccgtc ggggacagat gtaagtacgt tgccattgat
      361 ggtcggaaag tccatgtcgg ggtagtcgtt ggtatccgtt accagcgaag cgccgccggt
      421 cattaacccg acatagttga agttcccgcc gatatagtaa acaccatcat tcttgacgat
      481 ggcattcaca gctccgtcgg tcgaactcat gttcctgatc agggattggc caatggtgtt
      541 gttggcgatc agaaaaaaac aacctgccca tagagcaatg cgtaaaatac tgttcatata
      601 aattggtgtg tagtcgtttg ttaaatcggg acgatcaatg tcccgttgtt gagtgattgg
      661 gggaatgttc atcagcctaa ttccgaaaag attcatcggg aattttgcag gtgcaattgg
      721 tagtgtcctt tccgttcgaa attgactaaa agccgcttcc agtaaggcct gcgaaaggag
      781 gggcaggttg aaacgaaact aggaattatc tcctgattcc tcaaaaaaat ccacatctca
      841 ttagccgact ttacctgttt gatcaggaga aaatgccgga atagagaaaa aacaaaaaaa
      901 acgcctcgtc caggacgagg cgttttaata acaatttaag atgcgtttac agtttcacca
      961 ctttaccagt gccaactacg ccattctgtt cagcacggat gatgtaggtg ccggcgggga
     1021 gttgcgtagc gtcgaggtgc tggatcgtga gaccctggcc aacgtactgg ttcatgcggg
     1081 tgaccacgcg acccgtcagg tccatgatct cgaggcggac attgccggct ttcttggcct
     1141 gcagacgtac acgaatcgtg ttggc
//
LOCUS       DAOTON010000919         1165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_98760_length_1165_cov_2.468468, whole genome shotgun sequence.
ACCESSION   DAOTON010000919 DAOTON010000000
VERSION     DAOTON010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98760_length_1165_cov_2.468468"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1165)
                     /locus_tag="P5292_13930"
     CDS             complement(<1..>1165)
                     /locus_tag="P5292_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HRS40274.1"
                     /translation="EVIKAKAEKVDGPKVLGKIDIEAETKAPKKTTSRKKKTDEPEAA
                     APAEVPAPTPIPTPTPVAETPVVPEAPVSPAPAVAAETPVTPAVPAAPVATPPATPET
                     PAKDADSEVHRVQFANLTGPTVLGKIELPTETRRKPAASSDGEKKKRKRTERKGGGTP
                     VPQDKQRGAGSTDRNAQQQKARKPGEKTEISDKEIQDKIKATLARLSGGGKSKASKIR
                     RAKREERAEERELESLEQGQKIIKVTEFVTANELAKLMNVTVTEIISTCMSLGLFVSI
                     NQRLDAETLSVVAEEFGFKVEFVSADVQDSVKVEEDSADDLKPRSPIVTVMGHVDHGK
                     TSLLDYVRKANVIAGEAGGITQHIGAYEVQLENGKKITFLDTPGHEAFTAMRAR"
BASE COUNT          158 a          341 c          368 g          298 t
ORIGIN      
        1 cgcgcgcgcg catggcggta aaggcttcgt gacccggcgt atcgaggaag gtgatcttct
       61 tgccgttctc gagctgtact tcgtaagcgc cgatgtgctg ggtgatgcca cccgcttctc
      121 ccgcgatcac gttcgccttg cggacgtagt cgagcaggga ggtcttaccg tggtcgacgt
      181 gacccatcac ggtgacgatc gggctgcgcg gcttgaggtc gtcggcgctg tcttcttcca
      241 ctttcacgga gtcctgtacg tcggcggaga cgaactcgac tttgaagccg aattcttccg
      301 cgaccacgct cagggtttcc gcgtcgaggc gttggttgat cgaaacgaac agaccgagcg
      361 acatacaggt cgagatgatc tcggtgaccg tgacgttcat cagcttggcc agttcattcg
      421 ccgtaacgaa ttcggtgacc ttgatgatct tctggccctg ttcgagtgat tccagttcgc
      481 gctcttccgc acgctcttcg cgcttggcac gtcggatctt ggaagccttc gattttccac
      541 caccgctcag gcgggccagg gtagctttga tcttgtcctg gatctccttg tcgctgatct
      601 cggttttctc gcccggcttg cgggctttct gctgttgggc attgcggtcc gtagagcccg
      661 cgccgcgttg cttgtcctgc ggaaccggcg tgccgccgcc tttgcgttcg gtacgcttgc
      721 gttttttctt ctcgccgtcg gatgacgcgg ccggcttgcg gcgcgtttca gtcggcagtt
      781 cgatcttacc cagcacggtc ggaccggtca ggttggcgaa ctgtacacgg tgaacttcgg
      841 agtcggcgtc cttggccggc gtctcgggag tagcgggcgg tgtcgcaacg ggtgctgcag
      901 gtaccgcggg cgtgaccggt gtttcggcag ccacagcagg agccggactt accggtgctt
      961 ccggaactac cggagtttcg gcaaccggtg tcggtgtcgg gataggagta ggagccggaa
     1021 cttcagccgg tgcggctgct tccggttcat cggttttctt cttacggctg gtggtcttct
     1081 tcggcgcctt ggtttcagct tcgatatcga tcttgcccaa taccttcggg ccgtcgacct
     1141 tctcggcctt ggctttgatg acctc
//
LOCUS       DAOTON010000920         1162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_99184_length_1162_cov_2.553749, whole genome shotgun sequence.
ACCESSION   DAOTON010000920 DAOTON010000000
VERSION     DAOTON010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99184_length_1162_cov_2.553749"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="P5292_13935"
     CDS             complement(<1..510)
                     /locus_tag="P5292_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40275.1"
                     /translation="MKKILAAIGVLSLSLNGMQAQESSKFNFSLARQLKHSRIQNREI
                     ALFVQGDASVVREKTEALGGTFKYSAGDIHAIRIPLSGIQELAACAEIRRIESNDLQL
                     EPLNDRMVEMNHVSEVHLGFNLPQGYQGDGVIMGIIDEGIDYTHPDFRDNSGRTRIAF
                     LWDQANINFD"
     gene            776..>1162
                     /locus_tag="P5292_13940"
     CDS             776..>1162
                     /locus_tag="P5292_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRS40276.1"
                     /translation="MSDDKKIIFSMVKVSKTFTTGKTVLKDIYLSFFYGAKIGILGLN
                     GAGKSTLLKIIAGLEKSFQGDVVFSPGYTVGYLSQEPELDPSKTAREIVMEGAQRVVD
                     LLKEFEEINNKFGEPMSDDEMNKLIER"
BASE COUNT          303 a          277 c          285 g          297 t
ORIGIN      
        1 atcgaaattg atgttggcct ggtcccaaag gaaggcaatg cgcgtccgcc cgctgttgtc
       61 gcggaagtcg gggtgcgtat agtcgatgcc ttcgtcgatg atgcccatga tgacgccatc
      121 tccctgatat ccttgcggca aattaaagcc gaggtggacc tcactcacat gattcatctc
      181 gaccatgcgg tcgttgagtg gttccaactg aaggtcgttg ctttcgattc gacggatctc
      241 ggcacaggct gccaactcct gaataccgct caacggtatc cggatagcat gaatatctcc
      301 ggccgaatac ttgaaggttc caccaagcgc ctccgtcttc tcgcgaacga cgcttgcatc
      361 accctgtacg aacaaggcga tctcgcgatt ctggatgcgg gaatgcttga gctgtcgggc
      421 cagcgaaaaa ttgaacttgg aagattcttg tgcctgcatt ccgttcaggg aaaggctcag
      481 cacgccgatt gcagcgagta tttttttcat tgggcttaaa ctcattccct atacccgaca
      541 gttgatgaaa atgttgcaga aaatcaatca gttcctttgc agttcaatag catctaactg
      601 cctaattatt aaagattttt accgaaattg gcgctccgaa aacgggaaag tcattcattc
      661 tggtaaaaat tcgaattgtt ggatcagggt gtttattcaa gtaattgatt atcaattaat
      721 tacataatag cgaatatcga ccctcccgtt cgaaatcaac aaagcaacgc tcactatgtc
      781 agacgataaa aagatcattt tttccatggt gaaggtctcc aagaccttta ctaccggtaa
      841 gacagttctc aaagacatct acctgtcctt cttctatggt gccaagatcg gtatcctggg
      901 tttgaacggt gccggtaaat caaccttgtt aaagatcatc gcagggttgg aaaaatcctt
      961 tcagggcgat gtggtttttt cgccgggcta caccgtcggt tacctgtcac aggagcccga
     1021 actcgacccg agcaagaccg cgcgcgagat cgtgatggaa ggcgcacagc gggtggtgga
     1081 tctgttgaag gaattcgaag agatcaacaa caagttcggg gaaccgatga gcgacgatga
     1141 gatgaataaa ctcatcgagc gt
//
LOCUS       DAOTON010000921         1160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_99481_length_1160_cov_2.131222, whole genome shotgun sequence.
ACCESSION   DAOTON010000921 DAOTON010000000
VERSION     DAOTON010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99481_length_1160_cov_2.131222"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1160
                     /locus_tag="P5292_13945"
     CDS             <1..>1160
                     /locus_tag="P5292_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40277.1"
                     /translation="YADKVGIYKFSIDQKGGTNPELVPIAEYVHNFGELPFWYLGGEY
                     SEEAEDSIAGDYEKDDKSDDDESAIYESFFHAAVPFWNEAINDHSDVTGAYRMHLWPQ
                     KWEVADECEYVEDDGTGRYPCNGGYIWNGQTKHKCPGCKGTGYKSATSPYETHLINRD
                     KFNDENNQISVPGVGYISVPTDATKMLEEKAENNLKKGLMALNMDVMNEIGLNQSGKA
                     KEMDRTELNDFLQRIADMMFEIHLQNIYYFFTKYMFGVTDPNKLEEIEPQIVKPTQFD
                     IYSSSELTNQFAEAKKAKLNSSYLQVKQAEIQNKEFQTNPQLLERLNLELSLDPLAEV
                     NPEDVSLKLSNGTITQETAIIHDNIKEFIERSMEEDAAFADKKPSEQKAVLL"
BASE COUNT          378 a          258 c          279 g          245 t
ORIGIN      
        1 cctatgcaga caaagtaggg atttacaagt tttccatcga tcagaaaggc ggcacaaacc
       61 cggaacttgt gcccattgcg gagtacgttc acaattttgg ggaattacca ttctggtatt
      121 tgggtggtga gtattcagaa gaagccgaag actcaatcgc cggagactac gagaaagacg
      181 acaagagcga tgacgacgaa agcgcgattt atgagtcgtt tttccatgcg gcagtaccgt
      241 tctggaatga ggcaattaac gaccattcag acgttacagg agcgtacaga atgcacctgt
      301 ggccccagaa atgggaagta gccgacgagt gcgaatacgt tgaggatgat ggaactggtc
      361 gatacccttg taacggaggg tatatctgga acgggcagac caagcacaag tgtcctgggt
      421 gtaagggtac gggctacaaa tcagcgacaa gcccatacga gactcaccta atcaaccgcg
      481 acaagttcaa cgacgaaaac aaccagattt cagttccggg tgttggttac atatccgttc
      541 ctacggatgc taccaaaatg ctggaagaaa aggccgaaaa caacctgaaa aagggtttga
      601 tggccttgaa tatggacgtt atgaacgaaa taggtcttaa ccaatcaggt aaagccaagg
      661 aaatggaccg gacagaattg aacgacttcc tgcaaaggat agccgacatg atgtttgaga
      721 tccacctaca aaatatttac tacttcttta ccaagtacat gttcggagtc accgatccga
      781 acaagctgga agaaatcgaa ccacagattg ttaagcccac acagtttgac atctattcgt
      841 caagtgagct tacaaaccaa ttcgcggagg ctaagaaagc caagttgaat tcctcgtacc
      901 tacaggtaaa gcaggcagaa attcagaaca aggaattcca gaccaatcca cagcttttgg
      961 aaagactcaa tcttgaactt tcgcttgacc ccctggcaga ggtaaacccg gaagatgttt
     1021 cattgaaact atccaatggg accattaccc aagaaaccgc gatcattcac gacaatatca
     1081 aagaattcat cgaacggtcg atggaggaag atgcagcatt cgccgataaa aagccaagcg
     1141 aacaaaaagc ggtccttttg
//
LOCUS       DAOTON010000922         1159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_99548_length_1159_cov_3.592391, whole genome shotgun sequence.
ACCESSION   DAOTON010000922 DAOTON010000000
VERSION     DAOTON010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99548_length_1159_cov_3.592391"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="P5292_13950"
     CDS             <1..823
                     /locus_tag="P5292_13950"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRS40278.1"
                     /translation="VRHPYPIRQWEKTQYGNLYTFDLEQPTYENGYPVNAYVCDIELR
                     EHWNKRSLHAFDSTDLRNQPLRWTLVRFLASKGWRKDGRAVDALSATEVASIEQGVAN
                     VNDQERSSLSGRIRQIVWEFERYQQGGNPSGHSVTQRLEFWKAAMGVIRSHPLFGTGT
                     GDLKEAYQAEYKRMGTQLRPEFRLRAHNQYLAVTAALGIFGLAVFLACWIYPVFAFGK
                     AKGLLVQAAWLITTISMLTEDTLETQAGATFAALFISLFLFAQPGNPAEERQEES"
     gene            complement(824..>1159)
                     /locus_tag="P5292_13955"
     CDS             complement(824..>1159)
                     /locus_tag="P5292_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRS40279.1"
                     /translation="ENPHDRFIPHDPARVGKLKGDLLHYSYFSVSEHIRQMNRFTDIA
                     SEAAYRGGKRTTWLALLFRPAVKFLRDYFFLAGFLDGYYGFIAARISAHANFLKYVKL
                     YELQRKKKS"
BASE COUNT          279 a          304 c          326 g          250 t
ORIGIN      
        1 cgtgcgtcat ccttatccga tccggcaatg ggaaaagacg cagtacggga atctttacac
       61 ctttgatctc gagcagccga cctatgaaaa cggttatccg gtcaatgcgt acgtatgtga
      121 tatcgaattg cgcgaacatt ggaacaaaag gagcctgcat gcattcgact ccaccgacct
      181 gcgcaaccaa cccttgcgtt ggacgctcgt ccgctttctc gcgtcaaaag gttggcgaaa
      241 agacggccgg gccgtggacg cgttaagcgc gaccgaagtc gcgtcgatag aacaaggtgt
      301 ggcgaacgta aacgatcagg aacggagcag tctcagcgga cgcatccgtc agatcgtgtg
      361 ggaattcgaa cgttatcagc aaggaggaaa ccccagcggc cattcggtca cccaacgcct
      421 tgaattttgg aaagccgcca tgggtgtgat ccgttcccat ccgcttttcg ggacgggcac
      481 cggcgacctc aaggaagcat accaggcaga atacaaacga atgggaacac aactccggcc
      541 ggaatttcgt ttgcgtgcgc acaatcaata cctggctgta accgcggcac ttggcatatt
      601 cggtttggcg gtatttctcg cttgctggat ttatccggtg ttcgcatttg gaaaagctaa
      661 gggattactg gttcaggcag cctggctgat caccacgatt tccatgctga cggaagacac
      721 gctcgaaacc caggcgggcg ccacgtttgc ggctttgttc atcagcttgt tcctgtttgc
      781 acaacccggg aacccggcgg aagagcgaca ggaggaatcc tgatcaggac ttcttctttc
      841 gctgcaattc gtacaacttg acgtacttca gaaagttggc gtgcgctgaa attcgtgcag
      901 cgatgaatcc gtagtagcca tcgagaaagc ccgccagaaa aaaatagtcg cgcaaaaact
      961 taaccgcggg ccggaacagc aaggccagcc aggttgtgcg ttttccgccg cgataggctg
     1021 cttccgaagc gatgtccgta aagcggttca tttgccggat gtgttcggag acactgaagt
     1081 acgaataatg caggaggtcg cccttcagtt taccgactcg tgcgggatcg tgtggaatga
     1141 accgatcgtg cgggttctc
//
LOCUS       DAOTON010000923         1158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_99668_length_1158_cov_3.761559, whole genome shotgun sequence.
ACCESSION   DAOTON010000923 DAOTON010000000
VERSION     DAOTON010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99668_length_1158_cov_3.761559"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="P5292_13960"
     CDS             complement(<1..1138)
                     /locus_tag="P5292_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40280.1"
                     /translation="MIGIQYCYLVTAVFDDRSESCASPEACASLRRELPVITNADVVT
                     TDAAAGQVFVAWGKPIELDTVHYPGPYEYRLFRSPGFVGPSFTQIATLVAPADTTFLD
                     NGINTVGSAWSYRVDFYYTNAGALTFKGSTTIASSVFLSVSPTDQANVLSWQEEVPWQ
                     NYRYDIFRQNPATLLFDSIASTTASTFTDTALTNGTEYCYFVRSIGTYGLPSLVDPIV
                     NRSQRVCGTPFDNVPPCAPDLEVFSSCNENRNELIWTNPNNTCADDVEKYYIYYSLTE
                     DGGFERIDSVIGANDTTYLHIDLPALSGCYRVTAVDDNGNETTDPETVCVDTCRQYVL
                     PSVFTPNKDGKNDLFHPCDSTTTLDLQLRNCPPYRNVKDIDLKLF"
BASE COUNT          274 a          290 c          343 g          251 t
ORIGIN      
        1 tgaatagctt caggtcaatg tccttgacat tccggtacgg cggacaattc ctgagctgga
       61 gatcaagcgt cgttgtcgaa tcgcaggggt ggaagaggtc gttcttaccg tccttgttcg
      121 gtgtgaatac cgaaggcagc acatattgac ggcacgtatc gacacaaact gtttcagggt
      181 cggtcgtttc gtttccatta tcatctaccg cggtgacccg ataacatcct gagagcgcgg
      241 gcaggtcgat gtgcaggtag gtcgtgtcgt ttgcgccgat caccgaatcg atccgttcga
      301 aaccaccgtc ttccgtaagc gaatagtaga tgtagtattt ctcgacatca tccgcacagg
      361 tattgttcgg gttggtccag atgagttcgt tccggttttc gttgcaggat gagaagactt
      421 ccagatccgg agcacagggt gggacgttat cgaagggtgt accacaaacc cgttgagacc
      481 ggttaacaat cggatccacc aaagaaggca agccataagt accaatgctg cgaacaaagt
      541 agcaatactc ggtaccgttg gtcaatgccg tatcggtgaa ggtggaagcc gttgtactgg
      601 cgatcgaatc gaaaagcaag gtggcgggat tctgccggaa gatgtcgtag cgataattct
      661 gccagggcac ttcctcctgc cagctcaaca cgtttgcctg gtcggtgggg cttacgctga
      721 ggaacacgct tgaagcgatg gttgtgcttc ctttgaatgt cagggcaccg gcattcgtgt
      781 aatagaagtc gacgcggtaa ctccaggcgg aacccactgt gttgattccg ttatcgagga
      841 aggtggtatc ggccggggcc accagggtcg cgatctgggt gaacgaaggg ccgacgaagc
      901 cgggagagcg aaacaagcgg tactcatacg gacccggata atggacagta tcgagttcga
      961 tcggtttgcc ccaggccacg aagacctgac cggccgcagc atcggtggtg accacatccg
     1021 cattcgtgat gaccggcaat tcccggcgca gggaggcaca agcctccggc gaagcacaac
     1081 tttccgaacg atcatcgaag accgcggtga cgaggtaaca atactgaata ccgatcacaa
     1141 gacctgcgcc gccgttgt
//
LOCUS       DAOTON010000924         1155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_100061_length_1155_cov_3.330000, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000924 DAOTON010000000
VERSION     DAOTON010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100061_length_1155_cov_3.330000"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="P5292_13965"
     CDS             complement(<1..664)
                     /locus_tag="P5292_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRS40281.1"
                     /translation="MPAERHLHIIAFDVPYPADYGGVIDIYHKIRALQQAGVRVHLHA
                     FTYVRQPAAELDDLCASVRYYPRRTNKTLLFHTFPYIVLSRQSDQLLADLQQDEYPIL
                     FEGLHTTWLLNEPALAPRRRVVRMHNIEHDYYRHLALVERNPFKRWYFNAEAEKLKSY
                     EAVLARAHAIAAIAPADALELSTRYARVDCVPAFHPHDEVAALPGRGDFALYHGNLSI
                     GEN"
     gene            complement(654..>1155)
                     /locus_tag="P5292_13970"
     CDS             complement(654..>1155)
                     /locus_tag="P5292_13970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRS40282.1"
                     /translation="GAEEAIRAMEFLDDFLLVFVGGGDVYEQLRLEVSSKGLSDRVIF
                     LPRQRPETLRAITRLADVGLSLDKDTNLNYRYSLPNKLFDYIQAGIPVIATSIPEVQR
                     IVEQYRVGGIVSDLRPEVLAGFIRDTFSDPGRIRTWKENAQLAADELNWDQEKTRLLD
                     IIEHAR"
BASE COUNT          257 a          306 c          338 g          254 t
ORIGIN      
        1 tgttctctcc gatactcagg ttaccatgat acagcgcgaa atcgccacgc ccgggcagcg
       61 cagccacttc gtcgtgtgga tgaaacgcgg gcacacagtc gacccgtgcg tatcgtgtgg
      121 acagttccag tgcatccgca ggagcgatcg cggcaattgc atgggcacga gcgaggaccg
      181 cttcgtagga tttgagtttc tccgcctccg cattgaaata ccatcgcttg aacgggttgc
      241 gttcgaccag ggcgagatga cggtagtagt cgtgttcgat gttgtgcatg cgcacgacac
      301 gtctccttgg cgccagggcc ggctcgttca gcagccaggt cgtatgcagg ccttcgaaca
      361 ggatggggta ttcgtcttgt tgcaaatccg ccagcaactg atcggattgg cgactcagga
      421 cgatgtacgg gaatgtatgg aagagcaacg tcttgttggt ccggcgagga tagtatcgga
      481 ccgacgcaca tagatcatcc aactcagcgg caggctggcg cacgtaggtg aacgcgtgca
      541 agtgaacgcg aacgcccgct tgctgaagtg cccggatctt atgatagata tcgatcacac
      601 cgccgtagtc ggccgggtac ggaacatcga aggcgatgat gtgcaggtga cgttcagcgg
      661 gcatgttcga taatatcaag caggcgggtc ttttcctgat cccaattcaa ttcgtcggcg
      721 gcaagttgtg cattttcttt ccaagtcctg atccgtccgg gatccgagaa ggtgtcgcga
      781 ataaaccctg ccagaacttc cggccggaga tcgcttacaa taccaccgac cctatactgt
      841 tccacgatgc gttgaacttc cggaatcgac gtggcgatga ccggaatacc agcctggatg
      901 taatcgaaaa gtttattcgg cagggagtag cggtaattca ggttggtgtc cttatcgagt
      961 gataagccga catcggccag gcgcgtgatt gcacgtaatg tttcgggacg ctgacgggga
     1021 aggaaaatga cccggtcaga cagtcccttg ctgcttactt ctaaccgtag ttgttcgtac
     1081 acatcaccac cgccgacgaa gaccaggaga aaatcgtcga ggaactccat ggcgcgtatc
     1141 gcttcctccg ctccg
//
LOCUS       DAOTON010000925         1151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_100598_length_1151_cov_2.769161, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000925 DAOTON010000000
VERSION     DAOTON010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100598_length_1151_cov_2.769161"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1151
                     /locus_tag="P5292_13975"
     CDS             <1..>1151
                     /locus_tag="P5292_13975"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRS40283.1"
                     /translation="QGYLRVRADGKVLELKKGLQLDRYKVHDIELVVDRLEVNDGATS
                     RLTESLTLGMKLGKGSVILFEPDQEKDHYYSRHLMCPTSGVSYDEPQPNTFSFNSPYG
                     ACRRCNGLGTISEVDLKSIIPDPKQTIKGGGIIPLGPQKDTWIFKQLIALGRKYNFNL
                     GTPIEEIPEESMNIILYGSDDVLNVAMDYSSGSTYHHSITWEGIVNFIIDQDNESASP
                     SLRRWIQGFMQQVECPECHGSRLKKEALYFRIANKNISELAHLDILQLSRWFERLEAD
                     LSARQKKIGHEVIKEIRKRLKFLLDVGLDYLTLHRSARTLSGGESQRIRLATQIGSQL
                     VGVLYILDEPSIGLHQRDNVRLIRSLQDLRDTGNTVIVVEHDKETILAP"
BASE COUNT          289 a          326 c          307 g          229 t
ORIGIN      
        1 agcagggcta tctccgtgtc cgtgccgatg gcaaagtgct ggaattgaaa aaagggctgc
       61 agctcgaccg ctacaaggta cacgatatcg aactggtcgt ggaccgtctc gaggtgaacg
      121 acggcgccac cagtcgctta acggaatcgc tgacgctcgg catgaagttg ggaaaagggt
      181 ctgtcattct cttcgaaccg gaccaggaga aagaccacta ttattcccga cacctgatgt
      241 gcccgacatc gggcgtcagc tacgacgagc cgcaaccgaa caccttctcc ttcaactcgc
      301 cctatggtgc ctgtcgccgg tgcaacggcc tcggcacgat cagcgaggtt gacctcaaga
      361 gcatcatccc cgaccccaag cagacgatca aaggcggagg tatcattccg ctgggaccgc
      421 agaaagacac ctggattttc aaacaactga tcgcgctggg aaggaagtat aatttcaacc
      481 tgggcacacc gatcgaggaa attcccgaag agtcgatgaa catcatcctc tacggttccg
      541 acgacgtgtt gaatgtggcc atggattact ccagcggatc gacctatcac cacagcatta
      601 cctgggaagg gatcgtcaat ttcatcattg accaggacaa cgaaagcgct tcgccgtcgc
      661 ttcgccgctg gatacaaggc ttcatgcaac aggtcgaatg tcccgagtgt cacggatccc
      721 gactcaagaa agaagcgctg tacttccgca tcgcgaacaa gaacatttcc gaactggcgc
      781 atcttgatat cctgcaattg agccggtggt tcgagcgtct ggaagcggat ctaagtgcgc
      841 gccagaagaa gatcggtcac gaggtcatca aagagatccg taaacggctg aagttcctgc
      901 ttgatgtcgg gctcgattac ctcacgctgc accgctccgc ccggacactc agcggcggcg
      961 aatcgcaacg catccgactg gctacccaga tcggatcgca attggttggg gtgctgtaca
     1021 tcctcgacga accgagtatc ggccttcatc aacgcgacaa tgtacggttg atacggtcac
     1081 ttcaggactt gcgagatacc ggtaataccg tgatcgtggt tgaacacgat aaggaaacca
     1141 tccttgcacc c
//
LOCUS       DAOTON010000926         1151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_100615_length_1151_cov_2.452555, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000926 DAOTON010000000
VERSION     DAOTON010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100615_length_1151_cov_2.452555"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1151
                     /locus_tag="P5292_13980"
     CDS             <1..>1151
                     /locus_tag="P5292_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40284.1"
                     /translation="IPTINDDVVIDAASFTATGQVLNLDSTFYYCNSMSWAGVQFNPT
                     FEGNGATLNLRGSIAFSAGMTLSNVNLVMSATTGSHTIDLAGQYAYAVTIDANASYSL
                     SSPLTCIGPVDLRAGSLNANAFDISCGGFNAGVGTTLVFGDITLSVNGGSNEIFTRTI
                     TLDVMPSGADQTSVHFNCVSGEMDCINNTTPFDSLIFQNAGTLRACQANYASATNTSY
                     ALNSILGTGEFSTSILLENCTINQLNGKLVYLMSGTTSSIYNLNLNSSCSDMGRLSTS
                     YAGAPPATLNAASGTISIDYAILEGIQASGGATFNANNVIDLGGNTGWNISALVTRDL
                     YWVGGTGDWNDPNHWSLSSGGPSAGCTPNRLDNVFFDANSLGAGDTVRL"
BASE COUNT          241 a          368 c          298 g          244 t
ORIGIN      
        1 ccatccctac gatcaatgac gatgtggtga ttgacgccgc ttcgttcacc gcaacgggtc
       61 aggtgctgaa cctcgactcc accttctact actgtaacag catgagctgg gcgggtgtcc
      121 agttcaatcc gacctttgaa ggcaacggcg ctacgctgaa cctgcgtggc agcatcgcat
      181 tctccgccgg tatgaccttg tccaatgtca acctggtgat gagcgccaca accggttcgc
      241 acaccatcga cctggccgga caatacgcgt atgccgtaac gatcgacgcg aacgcgtcct
      301 actccttgtc ttcgccactg acttgcatcg gtccggtcga cctgcgtgca ggaagtctca
      361 acgcgaatgc ttttgatata tcctgtggcg gattcaacgc gggcgtaggc accacgctgg
      421 tgtttggcga catcacgctt tcggtcaacg gcggaagcaa cgagatcttt acgcgcacga
      481 tcacgttgga tgtcatgcca tccggcgccg atcaaacctc cgtgcatttc aattgcgtaa
      541 gcggcgaaat ggattgtatc aacaacacca cgccgttcga ctccctaatt ttccagaatg
      601 ccggaacgct gcgagcctgc caggccaact acgcgagcgc gaccaatacc tcgtacgcgc
      661 tgaactcgat tctcggcacc ggcgaattct ccaccagtat cctcctggag aattgtacga
      721 tcaaccaact caacggtaag ctcgtgtacc tgatgtcggg caccacctcg agcatctaca
      781 acctcaacct gaattcatcg tgctccgaca tgggccgact gagcacgtcc tatgccggcg
      841 ctcctcccgc aacactgaac gcggcttccg gcacgatctc catcgactac gccatcctcg
      901 aaggcatcca ggccagcggc ggggcgactt tcaatgcgaa caacgtcatc gacctgggcg
      961 gcaacaccgg atggaatatc tcggcgctgg taacgcgtga cctttattgg gtgggcggta
     1021 ccggcgactg gaatgacccg aaccactggt ccctctcgag cggcggtcct tccgccggct
     1081 gtacgcccaa tcgactggat aatgtcttct tcgacgcgaa ttcgctcggc gctggcgata
     1141 ccgttcgtct t
//
LOCUS       DAOTON010000927         1147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_101170_length_1147_cov_2.995421, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000927 DAOTON010000000
VERSION     DAOTON010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101170_length_1147_cov_2.995421"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1147)
                     /locus_tag="P5292_13985"
     CDS             complement(<1..>1147)
                     /locus_tag="P5292_13985"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40285.1"
                     /translation="SATQCGPGSLTLTASAAQTIHWYSVPVGGVELATGTSFTTPPIG
                     VTTNYYVEAGDYCRSSRTLVQAVINPIPSPPSLTDAGRCDPGSVVLLASSPEAIYWFD
                     QAVGGTQVGAGASYNTPSLSATTAFYVETGDICRSVRDTVYAFIETTPALPVATGDSR
                     CGQGAVTLSATATADLHWFDVPSNGAELGTGSSFTTPVLTSTTTFYVEAGNTCRSARV
                     PVVATILTAPTTPVASDVNNCGDGSLTLTASSNGTMTWYDQPTGGTVVGTGGTFITPM
                     LNTTTVYYVEASNGTCTSPRVPVQAIIETQPAVPVITDAIICGLGSAILLATSPVQVT
                     WYDQATGGTALSTGASYQTPVLSATTTYYAEAGVACRSNRVPATVTIL"
BASE COUNT          250 a          306 c          367 g          224 t
ORIGIN      
        1 aggatggtaa cggtggccgg aacacgattg ctacgacaag cgactcccgc ttcggcgtag
       61 taggtagtcg tggcactcaa gacgggagtc tggtaggatg cgccggtact caaagcggtt
      121 ccgccggtag cctgatcata ccaggttacc tgtaccggag atgtcgcaag aagtatggcc
      181 gagcccaggc cgcagataat ggcgtccgta atgacaggaa ccgcgggttg cgtttcaatg
      241 atcgcctgaa caggaacgcg cggactggtg caggtgccgt tacttgcttc gacgtagtaa
      301 accgtggtcg tgttcagcat gggcgtaatg aaggtgccgc cagttccgac aaccgtgccg
      361 cctgtgggct gatcatacca ggtcatcgtt ccattcgagg aggccgtcag ggtgaggctt
      421 ccgtcaccgc aattgtttac atccgacgcg acaggagtgg tcggtgcggt aagaatcgtt
      481 gctacaaccg gaacacgagc actgcggcaa gtatttcccg cttcgacgta gaaggtcgtt
      541 gtggacgtca gtacaggagt ggtgaaactc gaaccggtac ccagttctgc tccgttcgag
      601 gggacatcaa accaatgcag gtcggctgtc gcagtggccg acaacgtcac agcgccttgt
      661 ccgcaacggc tgtcaccggt agcaaccggt aatgccggag tggtttcaat gaaggcatat
      721 acggtatccc gcacgctccg gcagatgtcg ccggtctcga cgtaaaaggc ggtggtagcg
      781 cttagtgaag gcgtgttata ggacgctccg gctccgacct gtgtgccacc cacagcttgg
      841 tcgaaccaat agatcgcttc cggtgaactg gcaagcaata ccacggatcc cggatcacag
      901 cgtcctgcat cggtaagcga cggaggagac ggaatcggat tgatgactgc ctggaccaac
      961 gtgcgcgagc tgcggcagta atcgccagcc tctacatagt aattcgtcgt gacgccaatc
     1021 ggcggggtgg taaatgaggt gccggtggcc aactccacgc cgccaaccgg tacggaatac
     1081 cagtggatcg tttgagcggc actggcggtc aatgtaaggg agccggggcc gcactgtgtc
     1141 gcggagg
//
LOCUS       DAOTON010000928         1145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_101474_length_1145_cov_3.577064, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000928 DAOTON010000000
VERSION     DAOTON010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101474_length_1145_cov_3.577064"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1145)
                     /locus_tag="P5292_13990"
     CDS             complement(<1..>1145)
                     /locus_tag="P5292_13990"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HRS40286.1"
                     /translation="YADADGDGYGNPLDTLRYCTLPSGYVANALDCNDNNNQVSPAAL
                     EVCNGIDDDCDLYIDEGCGPEIYCIGPSASYTPPSGPSYINQFSPFPTLSAAVFALNS
                     MPATGHRIFEFQNNYTSGSETFPIHVIYAGTATATATFRPRSDAASTIVVSGSLGSTF
                     GLLSFIGTDYVTFDGSPGGTMGTTIRLAFRNTYPLTSGPTVSFIENATNIRLNALQLQ
                     GGHAASSVIRIGASSGPVGNRELSFTNCEIRDDVNGILTIPDRGIFSDNSTGTGLLSN
                     SGVTISNNNFINLPDGIIVGPNGANGRWDVLSNHFFFNRSGTVKLSEGIFIQSNDTLS
                     LRIENNFIGGTQPSAGGSPMAETNASLAFAGIYVNIANASLVASVKG"
BASE COUNT          272 a          296 c          305 g          272 t
ORIGIN      
        1 tacccttgac ggatgcgacc agagaggcat tcgctatatt gacgtagatg cccgcaaatg
       61 caagactggc attcgtctcc gccatcggac tgccgcctgc tgacggctgg gttcctccga
      121 tgaaattgtt ttcaatcctg agcgaaagtg tatcatttga ttgtatgaaa atgccttccg
      181 atagttttac cgtacccgat ctgttaaaaa agaaatgatt ggatagcaca tcccatcggc
      241 cattggctcc attcggcccg acaataattc catcgggcag gttgatgaag ttgttgttgc
      301 tgatggtgac gcccgaattg ctcagcaaac cggttcctgt actgttatcg gaaaatattc
      361 cccggtcggg tatcgtcaga ataccgttga catcatcgcg gatctcacaa ttggtaaaac
      421 tcaactcccg gttgcccacc ggcccggaag aggctccgat ccgaatcacg gagctcgctg
      481 catgacctcc ctgcagttgg agagcattca gccgaatgtt ggtggcattc tcgataaaac
      541 ttaccgtagg tccggatgta agagggtacg tattccggaa ggccaggcgg atggttgttc
      601 ccatcgtacc tcccggtgat ccgtcgaagg tcacataatc ggttccaata aaggagagaa
      661 ggccgaatgt cgaccccaga cttccgctga caacaatcgt tgaagcagcg tcgctccttg
      721 gccggaacgt ggctgttgcc gtcgcggtgc cggcatagat gacgtgtatt gggaacgttt
      781 cactaccgga ggtataatta ttctggaatt cgaaaatccg gtgcccggtc gcgggcatgc
      841 tgttcaatgc aaataccgct gctgaaaggg tcggaaacgg tgagaactga ttgatgtaag
      901 aaggtcccga aggcggtgta tagcttgccg atggtccgat gcaatagatc tccggaccac
      961 agccttcgtc gatatacagg tcgcaatcgt cgtcaattcc gttgcacact tccaaagccg
     1021 cgggagacac ctggttgtta ttgtcgttgc aatccagcgc attcgccaca tagccggacg
     1081 gaagcgtaca ataacgcagt gtatccagcg ggttgccata gccgtcgcca tccgcatcgg
     1141 cataa
//
LOCUS       DAOTON010000929         1145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_101539_length_1145_cov_2.037615, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000929 DAOTON010000000
VERSION     DAOTON010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101539_length_1145_cov_2.037615"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..773
                     /gene="upp"
                     /locus_tag="P5292_13995"
     CDS             126..773
                     /gene="upp"
                     /locus_tag="P5292_13995"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRS40287.1"
                     /translation="MVHNLSQSNSLINEFVSELRDTAIQGDRMRFRRNLERIGEIFAY
                     EISKTLPYVSRDVTTPLGIANMQVLREQPVVGTILRAGLPLHQGVLNYFDRADNAFIS
                     AYRKHHKDGTFDIKLEYVSCPSLENRVLILADPMLATGSSMVMTAKAIRERGNPSHTH
                     IVAAVASTYGVEFVKSHLPENVTIWCAAIDDVLTAQSYIVPGLGDAGDLAYGTKL"
     gene            824..>1145
                     /locus_tag="P5292_14000"
     CDS             824..>1145
                     /locus_tag="P5292_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40288.1"
                     /translation="MEELLKILSLILISSVKFAIGPPLVYLTEEYDFTWLETNLYAIL
                     GGMIGVVFFMNFSDWLIMGWNKLRDYFRKKPEPVKDQVFTDPIADTDEPIHIHYSYVE
                     HGSVV"
BASE COUNT          251 a          329 c          301 g          264 t
ORIGIN      
        1 ggctcggtcg atcccaatct cgcacgattc taccgaagct tcggttcgga ggaagtcgta
       61 tatttacaaa tccgaaggaa tcggcttccg ttcccgttcc ggctattcaa atcctgaccc
      121 gacgtatggt ccacaacctc agccaatcga actccctcat caatgaattc gtatcggaat
      181 tgcgcgatac cgcgatccag ggagaccgga tgcgctttcg ccgtaacctg gaacgtatcg
      241 gggagatctt tgcttatgag atcagcaaga cgctgccgta tgtcagtcgt gacgtgacga
      301 caccgctggg tatcgccaac atgcaggtgt tacgggaaca accggttgtc ggtacaatcc
      361 tgcgcgccgg ccttccgctg catcaaggtg tactgaacta cttcgatcgc gccgacaacg
      421 cgttcatcag cgcttatcgg aagcaccaca aggacggcac ttttgacatc aagttggaat
      481 acgtctcctg cccttcgctt gaaaaccgtg tgctgatcct ggccgacccg atgctggcta
      541 ccggttcgtc gatggtcatg accgccaaag cgatccggga gcgtggcaac ccgtcgcata
      601 cgcatatagt cgccgctgtt gccagtacgt acggcgtgga attcgtcaag agtcatttgc
      661 ctgaaaacgt cacgatctgg tgcgccgcga tcgatgacgt gttgaccgcg cagtcctaca
      721 tcgttcccgg tctcggcgat gcgggcgatc ttgcttacgg cacgaaactt tgatcccggc
      781 cgggggttga tatctatagt aagcactgac tgctgcccca ccggtggagg aactcctgaa
      841 aatattgtcc ctgatcctga tcagcagcgt caagttcgcg atcggacctc cgcttgtata
      901 tctcaccgag gagtatgatt tcacctggct ggagaccaac ctgtacgcga tactcggcgg
      961 catgatcggt gtggtgttct tcatgaactt cagcgactgg ctgatcatgg gttggaacaa
     1021 gctacgcgac tatttccgaa aaaagcccga gccggtcaaa gaccaggtct tcaccgatcc
     1081 aatcgccgat accgacgaac cgatccacat ccattattcc tatgtcgagc atgggtccgt
     1141 cgttg
//
LOCUS       DAOTON010000930         1143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_101850_length_1143_cov_1.870404, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000930 DAOTON010000000
VERSION     DAOTON010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101850_length_1143_cov_1.870404"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="P5292_14005"
     CDS             complement(<1..1066)
                     /locus_tag="P5292_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40289.1"
                     /translation="MLSSNLIEDITLPNITNTSAAFTGITYTNAVLNNQQVLIDSNTV
                     RDINYTGRGNFRGYMLTPSPVSTLPNRVSRNVLEHIVVGNGTSSLFRGIYCDQGFTDL
                     DYNRVGDSTIANDIQVAGGSIMYGIFLSNTPNSVSRIRNNLVTNVTLTNPGSSVFTGL
                     HYYGSGLGVHEVKDNLVQQISTLSTNPTTESSGSLAYALVGISAAADGGGTLTFDGNT
                     VQELTVATTAAANPGVVGFFVSRSNSASAATVSNNQVYKLYNHAIGAGPAPLMTGFRI
                     HNTHDDPLYFSNNRIALSNGTSATPLNMYGIYDGVIGGVVVRTKYYHFNSVSIMGSGS
                     GRSAAFWHNSSSANIEIRNNI"
BASE COUNT          286 a          277 c          298 g          282 t
ORIGIN      
        1 aaatgttgtt tcggatctcg atattggcgc tgctcgagtt atgccaaaaa gctgccgaac
       61 gaccgctacc gcttcccatg atggagacgg agttgaaatg ataatacttg gtccggacaa
      121 caacacctcc aatgacacca tcataaattc catacatgtt cagtggagta gccgacgtgc
      181 cattactcag tgcgattcgg ttgttcgaaa agtacagtgg atcgtcatga gtgttgtgta
      241 tccggaaacc cgtcatcagg ggtgcgggac ccgcgccaat agcatggtta tagagtttat
      301 atacctggtt attggatacg gtagccgccg aggcagaatt ggatctggag acgaaaaagc
      361 caacgacacc cgggttagca gctgcggtag tggccaccgt cagttcctga accgtattgc
      421 catcgaacgt cagtgtccca cctccgtcgg ctgccgcgga aatgcctacc agagcataag
      481 caagagaacc ggaagattcc gtggtaggat tcgtggaaag cgtcgaaatc tgctgcacca
      541 gattgtcttt cacttcgtgc acgcccaatc cggagccata gtaatgtaat cctgtaaaca
      601 cactgctacc tggattggtc aaggttacat tcgtgaccag gttgttcctt atacgactga
      661 ctgaattcgg agtgttggag aggaagatcc cgtacatgat acttccacct gcaacctgaa
      721 tatcattggc aatcgtactg tcacctaccc gattgtagtc cagatcggta aatccctgat
      781 cacagtagat ccccctgaat aaactcgacg tcccgttacc gacaacgatg tgttccagaa
      841 cgtttcggga tacccggtta gggagcgtgg aaaccggcga aggcgtcaac atataacccc
      901 ggaagttacc cctgcccgtg tagttgatat cgcgtaccgt attgctgtcg atgagaacct
      961 gctggttgtt cagcactgca ttggtatacg tgattccggt gaacgccgca ctggtgttag
     1021 tgatattggg cagcgtgatg tcttcgatca ggttggaaga aagcatgaca ctctgggatg
     1081 tctggtcaga gatgttctcg attcctttga atgcgatcga ttgggtactc gaagcagcaa
     1141 tgt
//
LOCUS       DAOTON010000931         1142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_102005_length_1142_cov_1.736891, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000931 DAOTON010000000
VERSION     DAOTON010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102005_length_1142_cov_1.736891"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>1142
                     /locus_tag="P5292_14010"
     CDS             19..>1142
                     /locus_tag="P5292_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40290.1"
                     /translation="MKSATEVFSGSDIDPAFHFEGIAAQQSFIKYDLIEGSAADPYHD
                     FPDIFVDLYGNVTNRTGIGYRTIAPLPGAANRNGNGAGNQVLVFPTFTSITPGTTTAG
                     TFSVITINGNNFGPAYGGTTNLEFPDANNGGAGYISTPANHIVSWTATSIQARVPSGA
                     GSGFIRVTNNLNETSVSPINLTISYNESNVVSGGVYYQPDLINQSGSGGYIYRYNTTF
                     NGNAAAVASFERALQTWRCATFVNFSRSGTSAISCQALDGTNLVTFDGACALPAGVLG
                     VSYSFYSGCGGGVWYVSENDLKFRTNGTGGITWQFGPAAPAFAEYDFESVCVHELGHS
                     HQLGHTILPVTVMNYALPNGTARRTLTAGSEVAGGNDVMTR"
BASE COUNT          279 a          307 c          279 g          277 t
ORIGIN      
        1 gagaccggtc tgtttttttt gaaatcagcc acggaggttt tttccgggtc cgatattgac
       61 cccgcctttc acttcgaagg aattgcagca caacaatcgt ttatcaaata cgacctgatc
      121 gaaggctctg ctgcggatcc ttatcatgac tttccggata tcttcgtgga tctatacgga
      181 aatgtgacga atcgtaccgg gatcggttac cgtaccattg ctccacttcc cggggctgcc
      241 aaccgtaatg gcaacggtgc cggtaatcaa gtactggtat ttccgacttt caccagcatc
      301 acaccgggaa caacaactgc cgggacattt tccgttatca ccatcaatgg taacaacttt
      361 ggtccggcct acggcggaac caccaatctc gaatttcccg atgccaataa cggaggagcc
      421 ggatatatct ccacaccagc caatcatatt gtcagttgga cggcaaccag cattcaagcc
      481 cgtgttccaa gcggagccgg aagtggtttt atccgtgtca ccaacaactt gaacgagacc
      541 agtgtctccc ccatcaacct cacgatcagt tacaatgaat cgaatgtcgt atccggcgga
      601 gtctactacc aacctgatct gatcaatcaa agtggttcgg gtggttacat ttatcggtac
      661 aatacgacct tcaatggaaa cgctgcagcc gtcgcttcct tcgaacgcgc attgcaaacc
      721 tggcgctgcg caacctttgt taacttttcc agaagcggga ccagcgccat cagttgtcag
      781 gcacttgacg gcaccaatct ggtgaccttt gacggagcct gtgcgctccc tgcgggagta
      841 ctcggcgtat cgtatagttt ctactccggt tgtggcggag gcgtttggta tgtaagcgag
      901 aacgatctga aattccgaac aaatggtacc ggtggtatca cctggcaatt cggaccggca
      961 gcgccggcgt ttgcagaata cgatttcgaa tcggtctgtg tacatgaatt gggacacagt
     1021 catcagctcg gccataccat ccttccggtt acggtcatga actatgcatt gccaaatgga
     1081 acagcacgtc gtacccttac tgcaggaagc gaagtggcag gaggcaacga tgtaatgaca
     1141 cg
//
LOCUS       DAOTON010000932         1141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_102055_length_1141_cov_3.520258, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000932 DAOTON010000000
VERSION     DAOTON010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102055_length_1141_cov_3.520258"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1141)
                     /locus_tag="P5292_14015"
     CDS             complement(<1..>1141)
                     /locus_tag="P5292_14015"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRS40291.1"
                     /translation="GFFGCAFSLIQEKVNVINGTYATIASQSRGFYLGYGLTGEIRVG
                     DSWINTGVQYPTDNAWHNLTVVRAVDNTYLYIDGVLAATAGFVIPSPGPVFDNPIPTF
                     YIGSQFYGNSEVWNGAIDEVRVWNRALCEREVAEVAGCEISGSADGLVAVYNFNQGLA
                     EEPNPTFDNMLLDASGNGRHATTVNFNWSGSASNWIRAGGVATGNSCSSPSYTVAYFD
                     QDEDNFGRSDMTVVYNSLCDVPPSYRLVAGDCDDNNPAIHPRLTLPTGAIAFLPFNGN
                     AQDESGTGNNGIVQGATLTSDRFGHANNAYEFNGFSSFIEILNDGDFNVSDLTLAAWI
                     RPDGAGSGTVMSKSFGVGGLSSFIMNADPGADRLDVVYGADPANDT"
BASE COUNT          268 a          311 c          308 g          254 t
ORIGIN      
        1 cgtgtcgttt gccggatcag cgccataaac aacatccagc cggtcggcgc cgggatccgc
       61 gttcatgata aaggaactaa gaccgccgac accgaaggat ttactcatga ccgttccact
      121 acccgctcca tccggacgga tccaggcggc caatgtcaga tcggaaacgt tgaagtcgcc
      181 gtcgttgaga atttcaatga aggaggagaa accgttgaat tcataggcgt tgttcgcgtg
      241 accgaaccga tcactcgtca gggttgcgcc ttgaacaata ccgttgttgc cggtaccgct
      301 ttcgtcctgc gcgtttccat tgaatggcag gaaggcaatg gctcctgtcg gaagggtcag
      361 tcggggatga atggccgggt tgttgtcgtc acagtcgcct gcaaccagac ggtaggacgg
      421 tggtacatcg caaagcgaat tgtacacaac ggtcatgtca ctccgaccga aattatcctc
      481 gtcctggtcg aaatacgcga cggtgtaaga cggcgaagaa caactgtttc cggttgccac
      541 accgcctgca cggatccagt tggaagcaga gccactccaa ttgaaattca cggtcgtggc
      601 atgacgacca ttaccactgg catccaacaa catgttgtcg aacgtaggat tgggttcttc
      661 cgcgagtccc tggttgaaat tgtaaaccgc gacgagtccg tcagcggatc cgctgatctc
      721 acaacccgcg acttcggcga cttctctttc gcacaatgcg cggttccaga cgcggacttc
      781 gtcgatcgct ccattccaga cttcggagtt cccataaaac tgcgatccga tgtagaaggt
      841 cgggatcgga ttgtcaaaca ccggtccagg gcttggaatg acgaatccag ccgtggcagc
      901 caatacgccg tcgatgtaga gataggtatt gtccacggcg cggactaccg tgaggttgtg
      961 ccatgcatta tcggttggat attgtacgcc cgtattgatc caggaatcgc cgacccggat
     1021 ctcgccggtt aagccataac ccagataaaa accccggctc tgtgatgcga tcgtggcata
     1081 cgttccgttg attacattga ctttctcttg gatcaggcta aaagcgcatc cgaaaaagcc
     1141 t
//
LOCUS       DAOTON010000933         1140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_102280_length_1140_cov_2.126267, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000933 DAOTON010000000
VERSION     DAOTON010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102280_length_1140_cov_2.126267"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1140)
                     /locus_tag="P5292_14020"
     CDS             complement(<1..>1140)
                     /locus_tag="P5292_14020"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40292.1"
                     /translation="AATTYQWQISTDSTNWSDIPGATSRDLPGTTIGALYQKTFFRRI
                     IGGDACILAGAADQVVTVKIVTASGTVTDAACNGTATGSISATSDGESPFTYAWSNGQ
                     TSQTAINLAAGTYTVTITDNGNCTATTSFTVGEPDALDATASTTTANCSSNTGGASVT
                     VTGGTQPYSYLWSNGASGSSISNVAGGSYSVTITDANSCTFTLPVTIPTTSIPDVNAG
                     LDQTLTCSNASITLTATSSAPGATFAWTGGPNGASKTVTAAGTYTVTATDPASGCTAS
                     DEVNISQDIATPNVDAGTDQSLSCTITSATLTATSSTPGVTFDWVGGPSGASKTVTAA
                     GVYTVIATNPDNGCTASDIVSVSQDISTPNVNAGADQTLTCGTTS"
BASE COUNT          210 a          296 c          373 g          261 t
ORIGIN      
        1 atgctggtcg tgccgcatgt caacgtctga tcagctccgg cattgacgtt cggggtgctg
       61 atgtcctggc tgaccgaaac gatgtcggat gcggtgcagc cgttgtccgg attcgtggcg
      121 attaccgtat agacaccggc tgcggtcacg gtcttggatg ctccgctcgg gccaccgacc
      181 cagtcaaacg taacgccggg tgtgctggaa gttgctgtta aagtggcgct ggtgatcgta
      241 cagctgagcg attggtcagt gccggcatcc acattcggtg tggcgatgtc ctggctgatg
      301 ttgacctcgt cagatgcagt acatccgctg gcaggatccg tggcggttac agtataagta
      361 ccggcagcag tgaccgtctt ggacgcgccg ttcggtccgc cggtccaggc aaaggttgcg
      421 cccggtgcag aggatgtcgc ggtcagtgtg atggatgcgt tgctgcaggt gagtgtttgg
      481 tcaagccccg cattcacatc gggaatgctg gtagtcggaa tggtcaccgg cagggtgaag
      541 gtgcaactgt tcgcgtcggt gatggttacc gagtaactgc cgcctgctac gttggaaatc
      601 gaagatcccg aagcgccatt gctccacagg taggaatacg gttgcgttcc gccggttacg
      661 gtcacgctcg ccccaccggt attgctgctg caattcgccg tggtggttga tgccgttgca
      721 tcgagtgcat ccggttcgcc gaccgtgaag gaggttgttg ccgtacagtt gccgttatcg
      781 gtgatggtca ccgtgtaggt gcctgctgcg aggttgatcg cggtctggct ggtctgacca
      841 ttgctccatg cataggtgaa aggtgattca ccatccgatg tagcggagat cgaaccggtg
      901 gcggtaccgt tacaggccgc atcggttacg gtaccggaag cggtaacgat tttcaccgtc
      961 acgacctggt cggcagcgcc ggcgagaata caggcgtcac caccgataat gcgacggaaa
     1021 aaggtcttct gatacagcgc cccaatggtg gtgcccggaa gatcacgtga agtcgcaccc
     1081 ggaatatctg accaattggt actatccgta ctgatctgcc actggtaggt ggtggctgcg
//
LOCUS       DAOTON010000934         1138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_102521_length_1138_cov_3.230840, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000934 DAOTON010000000
VERSION     DAOTON010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102521_length_1138_cov_3.230840"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1138
                     /locus_tag="P5292_14025"
     CDS             <1..>1138
                     /locus_tag="P5292_14025"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HRS40293.1"
                     /translation="AIFNFGVKITANNPVDVFAYHHRLYFSDASIVLPIDEMATEYTV
                     MAQEDLSGNSPSEFVVLATRDSTVLEITPSDVTINFRPPNVPFNVRIDEGQTFQLQSY
                     GDLTGSRVRVLSGGKVAVFGGARQAHVHCLGADNTLYDECYPIERSGKFYVTVPFLGQ
                     GGDIFRTLSLQDSCILSINGTTVTTLNRGEYFDTLLATASTFSSTKLILMSQFNKSQQ
                     CNSSMLGDPSMVNLVPLELKKEDVSWISLDGPSGQQYVSSHFVNILTATSGVNALQLD
                     GNTVNFSPVPSLPGFSFARVAISSGPHRLTSPLGFQAVVYGFGDYNAHTFHLGYDDPD
                     SPVGVLDFESDGTLVEWPEGGSVRITLSDALLALKGTLRLELFDA"
BASE COUNT          244 a          324 c          301 g          269 t
ORIGIN      
        1 agccatcttc aattttggcg tcaagataac cgccaacaat cctgtggatg ttttcgcgta
       61 ccatcatcgc ctctacttct cggacgcgtc aatcgtattg ccgatcgacg agatggcgac
      121 ggaatacacc gtgatggccc aagaggatct gtctggaaat tcaccctccg aattcgtggt
      181 gctggccaca cgagacagta ccgtattgga aataacgcct tcggacgtga ccatcaactt
      241 ccgcccgccg aatgtaccct tcaacgtacg catcgacgaa gggcaaacct tccagttgca
      301 atcgtacggt gacctgaccg gcagccgggt gcgggtactc tccggcggaa aagtcgccgt
      361 gttcggcggc gcccgtcagg cgcatgtaca ttgtctggga gccgacaata ccttgtacga
      421 cgaatgttat cccatcgaac gttccgggaa gttctatgtc actgttccgt ttctcggtca
      481 aggcggtgat attttccgaa cgctttccct gcaggacagt tgcatcctga gcatcaacgg
      541 cacgacggtg actacgctga atcgcggaga atacttcgac accctgctgg ccacggcttc
      601 cacgttcagt tcgacgaagc tgatcctgat gagtcagttc aacaagagcc agcagtgcaa
      661 tagcagcatg ctgggcgatc cgtcgatggt gaatctggtt ccccttgaat tgaaaaaaga
      721 agatgtatcc tggatctcac tggatggccc ttcggggcaa caatatgtga gcagccattt
      781 cgtgaacatt ctcacagcta cttccggcgt aaacgcattg caattggacg gcaacacggt
      841 caacttttct ccggttcctt ccctgcccgg cttctccttc gcccgagtcg cgatcagctc
      901 cggcccgcac cgactgacca gtccactcgg tttccaggcg gtggtctatg gattcggtga
      961 ttacaatgcc catacctttc acctgggtta cgatgatccg gacagtccgg tgggtgtgct
     1021 cgatttcgaa tcggacggca ccctggtcga atggccggaa ggcggttcgg tgaggatcac
     1081 tctttccgat gcattgctgg ctctgaaagg aacgcttcga ctggaactgt tcgatgcg
//
LOCUS       DAOTON010000935         1137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_102642_length_1137_cov_3.704251, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000935 DAOTON010000000
VERSION     DAOTON010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102642_length_1137_cov_3.704251"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="P5292_14030"
     CDS             complement(<1..503)
                     /locus_tag="P5292_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRS40294.1"
                     /translation="MARNFISNKDETVRMFQNDLLDKLTRIHFSVPLFIFVPVILYFI
                     YQSFFTLGLTILAFGAYLVFGLFIWTLTEYVLHRFVFHWCPPGKWGERIHFIFHGVHH
                     DYPSDSKRLVMVPTVSIPLAALFFWLFRLMIGPVHVAPFFVGFLCGYLFYDMTHYAIH
                     HYNFKSK"
     gene            complement(547..>1137)
                     /locus_tag="P5292_14035"
     CDS             complement(547..>1137)
                     /locus_tag="P5292_14035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRS40295.1"
                     /translation="SVSVRDACGSTPARDSVLVTLRPAPVSSFIYDPVEPFSFDNTVS
                     FIDQSVNATSWTWIIADSDTVMTRNPVYTFEQPGTYNVTLITVSGNGCTDTLTYRIFI
                     REEIAVYYPNSFTPNKDGKNDLWLPIGQSLGTFTYAIYDRWGQIIFEGDEAHPWPGTY
                     KDSDRLIPEGVYVYRVDLKDDKFDPKVVAGRVTLIR"
BASE COUNT          312 a          275 c          314 g          236 t
ORIGIN      
        1 aacttgctct tgaagttgta atggtggatg gcatagtggg tcatgtcata aaacaaatac
       61 ccacacaaaa agccgacgaa gaagggagcg acgtgaacag gtccgatcat cagccggaac
      121 agccagaaaa agagtgctgc cagcggtata ctgacggtcg gaaccatgac cagtcgtttg
      181 ctatcgctcg gataatcgtg gtgtactcca tggaaaatga agtggatccg ttctccccat
      241 ttaccgggcg gacaccaatg aaagacaaag cggtgcagaa cgtattccgt cagggtccag
      301 atgaataggc cgaacacgag gtacgcaccg aacgcgagga tcgttaaccc taatgtgaaa
      361 aacgactggt aaatgaaata aagaatgacc ggaacgaaga tgaacagtgg cacggagaaa
      421 tggatccgcg tcagtttatc cagaaggtca ttctgaaaca tccggactgt ttcgtccttg
      481 ttgctgatga aattcctggc cacgacaata cgaaattaag cctggggaag gaatccggca
      541 agctgtttaa cggatgaggg taacccgccc ggcgacaacc tttggatcga atttatcgtc
      601 tttcaggtca acccggtaca catatacccc ttcggggatg agacggtcgg aatctttgta
      661 cgtgcccggc cagggatgcg cttcgtcccc ttcgaaaatg atctgtcccc accgatcgta
      721 gatcgcgtag gtgaaggtgc cgaggctttg tccgatcggc agccaaaggt cattcttgcc
      781 atccttattc ggcgtgaagc tgttcggata ataaacagcg atctcttccc ggatgaagat
      841 tcggtaggtc agggtatctg tgcatccgtt cccgctgacg gtaatcaagg tgacattgta
      901 ggtaccgggc tgttcgaacg tatagaccgg attccgggtc atcacggtat ccgaatcggc
      961 aatgatccag gtccaggagg tcgcattcac tgattgatcg atgaacgaca ccgtattgtc
     1021 gaaggagaaa ggctcgaccg gatcgtagat aaacgacgaa accggagcag gtcgcagcgt
     1081 caccaatacc gaatcacgcg ccggggtcga accacaggca tcgcggacgg aaaccga
//
LOCUS       DAOTON010000936         1136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_102789_length_1136_cov_2.969473, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000936 DAOTON010000000
VERSION     DAOTON010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102789_length_1136_cov_2.969473"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1136
                     /locus_tag="P5292_14040"
     CDS             <1..>1136
                     /locus_tag="P5292_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40296.1"
                     /translation="FIDQFKWFMDQKEIELSSSSTAAVQDTATGISLTGSDFISVEAR
                     QDSLRWKAPFARYSLKDFLIKAEKVAFIQTADASVIPDSGKVVVERYAKMRTLTDARV
                     LANNTTKYHTIYNANIDIQGRKRFSGTGDYDYIDENKVKHHFHFDEIGVDTTFQTVAR
                     GQLADTAGFPLSPQFLFKGDVRLTASKEYLNFSGFTKPNIQCEKIGRNWIRFSGDINP
                     ANVSIPIASPVTDDGAKLSAAVAQTSDSTGIYAAFLMPKQRSSDLEIISASGVLYFDK
                     ASKQFRITTAERLEKPAAAGNYLSLDDARCFVYGEGRLNFGSDFGQLNLTTVGNVINN
                     LVNDSTQFEIMAAIDFPFSDDALKVMSDQLSTNPNLQATQDLGRP"
BASE COUNT          280 a          327 c          284 g          245 t
ORIGIN      
        1 ttcatcgacc agttcaagtg gttcatggac caaaaggaga tcgaattgag ttcatcttcc
       61 acggcagctg tgcaggatac ggctaccgga atttcgctca ccgggtccga cttcatctcg
      121 gttgaggccc ggcaggattc cctgcgctgg aaagccccgt tcgcgcgcta ctccctgaag
      181 gacttcctga tcaaagccga aaaggtcgcc ttcatccaaa ccgccgacgc ttcggtcatt
      241 ccggatagcg gaaaggtcgt agtggaacgc tacgccaaaa tgcgcacgct caccgatgca
      301 cgggtgctgg ccaataacac gaccaaatac catacgatct acaacgccaa catcgacatt
      361 cagggacgca aacgcttcag cggtaccggt gactacgact acatcgacga gaataaagtg
      421 aaacaccatt ttcattttga cgagatcggc gtggacacca ctttccaaac cgtagcccgg
      481 ggacaactgg ccgataccgc cggattcccg ctcagtccgc agttcctgtt taaaggagat
      541 gtgcgcttga cagcttcaaa ggagtatctc aatttctcgg gctttaccaa acccaacatc
      601 cagtgtgaga aaatcgggcg taactggatc cgattcagtg gtgatatcaa cccggccaac
      661 gttagcattc cgatcgcttc acctgtgacc gacgatggag cgaagctttc cgcggctgtg
      721 gctcagacgt ccgacagcac cggcatttac gccgccttcc tcatgcccaa gcagcggtcc
      781 agtgacctgg agatcataag tgcttccggt gtgctgtatt tcgataaagc gagcaagcag
      841 ttccggataa cgactgcaga acgacttgaa aaaccggccg cagccggtaa ctacctgagc
      901 ctggatgatg ctcgttgctt tgtttatggc gaaggaaggt tgaatttcgg atccgatttc
      961 ggccagctca acctgactac tgttggtaat gtgatcaata atctcgtcaa cgacagtacg
     1021 cagttcgaga tcatggccgc catcgatttc ccgttctccg atgacgcctt gaaggtcatg
     1081 agcgatcagc tatccaccaa tccgaacctt caggcaaccc aggacctcgg ccgacc
//
LOCUS       DAOTON010000937         1134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_103055_length_1134_cov_3.615385, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000937 DAOTON010000000
VERSION     DAOTON010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103055_length_1134_cov_3.615385"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1134)
                     /locus_tag="P5292_14045"
     CDS             complement(<1..>1134)
                     /locus_tag="P5292_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40297.1"
                     /translation="PWVNAIGGSGATTASYTSPSLTGAPIYYRLKVSCSGNDAYSNSC
                     YVSASDCQFDVTYNTGISYSSIAGTGSNYSFPGTTTDDQTSNTLSLAGTTFQYKGQTV
                     TGLKACTNGWITFNTASTSTEYSNNIGTSSTTLRAILAPFWEDLVCTGNPGTTAGLNA
                     SMKYEIVGSLGSGSAQIICEWIGMETYSNAGPNLNFQVVLDENGNTITYNYGTMEGFN
                     GSANFGYAYTIGMNGWSFATPGSYTDVMALRDDFTTNFDYTPSTTANSGLNSKNYLPA
                     CNSQYVFNPVTNYAGPGSAPTYAPGNDESGSAFALSVNAGPCAQYCGTYYEGRGATAS
                     SGIPVCSAGSPGTPDDDVWFSFNATTSSQTITVRGGGGYDAVV"
BASE COUNT          269 a          280 c          342 g          243 t
ORIGIN      
        1 ggacaaccgc gtcataaccg ccgccaccac gaaccgtaat ggtttgagaa gatgtggtag
       61 cgttgaagga gaaccataca tcgtcatccg gagtaccggg agaacccgcg gaacagaccg
      121 ggattccgct ggaagccgta gcaccgcgac cttcgtagta ggtgccgcaa tactgggcac
      181 agggtccggc attcaccgac aaggcaaaag cagaaccgga ttcgtcgttg ccaggagcat
      241 aggtcggggc tgaacccgga cccgcgtagt tggtcactgg gttgaacaca tactggctgt
      301 tacaagccgg cagatagttc ttcgagttca ggccggagtt ggccgtggtc gaaggggtat
      361 agtcgaagtt cgtggtgaaa tcatcccgta gcgccatgac atcggtatag ctgcccggcg
      421 tggcaaagct ccatccattc atgccgatgg tgtaagcata accgaagtta gcgctgccgt
      481 tgaagccttc catcgtacca tagttgtagg tgatggtgtt gccgttctca tccagcacga
      541 cctggaagtt cagattcgga cctgcgttcg agtaggtctc catgccgatc cattcgcaga
      601 tgatctgcgc ggaaccggat ccaaggctgc caacgatctc gtatttcatc gaggcgttga
      661 gaccggcggt agtaccgggg ttaccggtac aaaccaagtc ttcccagaaa ggagccagga
      721 ttgcacgaag ggtggtggag gatgttccga tgttgttact gtattccgtc gaggtggatg
      781 ccgtgttgaa cgtgatccag ccgttggtac aggccttcaa gccggtgaca gtctgtcctt
      841 tgtactggaa ggtggttcca gccagcgaaa gggtgttcga tgtctgatca tccgtggtag
      901 tgccggggaa gctatagttc gatccggtgc cggcaatcga agagtacgaa atacccgtgt
      961 tgtaagtcac atcgaactga cagtccgaag cactcacgta acaagaattc gaataggcat
     1021 catttccaga acaactcact ttcaatcggt agtagattgg agcgccggtg agcgaaggag
     1081 aagtataaga ggcggtggtg gctccggagc caccaatagc gttcacccaa gggt
//
LOCUS       DAOTON010000938         1130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_103643_length_1130_cov_3.192558, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000938 DAOTON010000000
VERSION     DAOTON010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103643_length_1130_cov_3.192558"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="P5292_14050"
     CDS             <1..1051
                     /locus_tag="P5292_14050"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40298.1"
                     /translation="TGLPFHSYSLTGPIYVCAGTNGVNYSIGSVTGADTYAWTITGDA
                     TVVNGNGPSATVNFGPSWTSGNLTVTTTNTCGAYQRTFTIRSVTAQPGGITGQASALC
                     NGTNVAYSISPVAGATSYSWSVPAGSTIVTNSGTAITVNFGSVAGNICVIANNACGAS
                     PARCLAVTLVPAQPGVISGPTSVCKSSTQTYSVASVSGASIYNWTVTGGAAIAPQGTS
                     AIVNFNSALSGSAIVKVSTGNACGFSPARSLAVVVNLACRTAGEPDQATAMTVFPNPA
                     RDRIRVQFAATGTLEYLLKVSDLLGKEVAIHRLSATEGRNEYEIDLSGLERGIYLFSL
                     MTTNGTPSVQRVIIE"
BASE COUNT          244 a          332 c          315 g          239 t
ORIGIN      
        1 cacgggtctg ccgttccatt cttattcact caccggaccg atctacgtct gtgccggaac
       61 gaacggcgtg aactattcca tcggaagcgt caccggcgct gacacgtatg catggaccat
      121 taccggcgat gctacggttg tgaacggcaa cggtccttcg gctacggtga acttcggtcc
      181 gtcgtggacc agtggcaacc tcacggtaac gacgaccaac acatgcggag cctaccagcg
      241 gacctttacg atccgttcgg tgactgctca gcccggtggc atcaccggtc aggccagcgc
      301 gctttgtaat ggtacgaatg tcgcctactc gatctctccg gtcgccggcg cgacgagtta
      361 ttcgtggtcc gtgcctgccg gttcaacgat cgtaaccaac tccggaacag ccattacggt
      421 gaacttcgga agtgtggccg gtaacatctg cgtgatcgcg aacaacgcct gcggtgcaag
      481 tcctgcgcgt tgcctggccg tcacgctcgt accggctcaa ccgggtgtaa tctccggccc
      541 gacctccgtt tgcaaatcga gcacgcagac ctactcggtt gcttcggtaa gcggcgcttc
      601 gatctataac tggacggtca ccggtggcgc tgccatagcg cctcagggaa cttcagccat
      661 tgtgaatttc aattcggcgc tatcgggttc agccatagtg aaagtcagca ccggcaacgc
      721 ttgcggattc agtccggcac gcagcctggc cgtcgtcgtt aacctcgcct gccgcacagc
      781 gggcgagccc gatcaagcga ccgcgatgac ggtattcccg aatccggcgc gtgatcgcat
      841 ccgggtgcag tttgctgcca cgggtacgct ggagtatctg ttgaaggtca gcgacctcct
      901 tggaaaagaa gtcgcgatcc atcgactgag cgctacagaa ggtcgtaacg aatacgagat
      961 cgacctgagc ggactcgaac gcggcattta cttgttctcg cttatgacta ctaacggtac
     1021 tccatcggta cagcgcgtga tcatcgaata gaacaaccag tattaagaaa agcacagggg
     1081 ttgtaagtag ctacttacaa cccctgtcta attaaaaaga ggagtgaacg
//
LOCUS       DAOTON010000939         1130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_103650_length_1130_cov_2.797209, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000939 DAOTON010000000
VERSION     DAOTON010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103650_length_1130_cov_2.797209"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="P5292_14055"
     CDS             complement(<1..1073)
                     /locus_tag="P5292_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRS40299.1"
                     /translation="MVITRFYLFEKPARMDTLITSNRTLDLSYKQNFFTFEFAALNFL
                     FPEKNRYMYKMENLNEDWIYAGDRRYATYTNIDPGTYTFRVKACNNDGVWNDEGISIL
                     ITIRPPFYSTWWFRALAAIAITLIIFLYIRLRTNALMKQNVILEDKVTQRTQELQEKN
                     VELTKTMDDLRATQDQLVQSEKMASLGQLTAGIAHEIQNPLNFVNNFSELSMELLDEL
                     GGVQDRSEQGEIIGDLKQNLEKIVFHGKRADGIVKGMLQHSRAATAEKQSTDINRLVE
                     EYCSLAYHGMRAKDPAFNCTIERNFASDLPHARIIPQDISRVILNIVNNAFYAVDERK
                     RSGDKQFKPTVSIATRREGERLL"
BASE COUNT          210 a          307 c          333 g          280 t
ORIGIN      
        1 atgagcagcc gttcgccttc ccgtcgcgtc gcgatcgata ccgtcggctt gaactgcttg
       61 tcgccggatc gctttcgttc atcgactgcg tagaaggcgt tgttgacgat gttgaggatg
      121 acccggctga tgtcctgcgg aatgatccgg gcgtgcggca gatccgaagc aaaattccgt
      181 tcaatggtgc agttgaatgc agggtccttt gcacgcatgc cgtgatacgc caggctgcag
      241 tactcctcga ccagccggtt gatgtcggtg gattgcttct cggcagtcgc ggcgcgggaa
      301 tgttgtaaca tgcctttcac gattccgtcg gcacgcttac cgtgaaagac gatcttttcg
      361 agattctgtt taaggtcccc gatgatctca ccttgttcgc tccggtcctg gaccccgcct
      421 aactcgtcca gcaattccat cgacaattcg gaaaagttgt tgacgaagtt cagcggattc
      481 tggatctcgt gtgcgatgcc ggcggtcagt tgaccgagcg aggccatctt ttccgactga
      541 accaattggt cctgggtcgc gcggaggtcg tccatggtct tggtcaactc gacgttcttc
      601 tcctgcagtt cctgcgtgcg ctgcgtcacc ttgtcttcga ggatgacatt ctgcttcatc
      661 agcgcgtttg tccgtaagcg gatgtagagg aagatgatca gcgtgatcgc gatggctgcc
      721 agcgcccgga accaccaggt gctgtaaaac ggcggccgga tggtgatgag aatggaaatg
      781 ccttcgtcgt tccaaacacc gtcgttgttg caggccttca cccggaaggt gtacgtgccc
      841 gggtcgatgt tcgtatacgt ggcgtagcgg cggtctcctg cgtagatcca gtcctcgttc
      901 aggttctcca tcttgtacat gtaccggttt ttctccggaa agaggaagtt cagcgccgcg
      961 aattcgaagg tgaagaagtt ctgcttgtaa ctcaggtcga gggtgcggtt cgacgtgatg
     1021 agcgtatcca tccgcgccgg cttctcgaag aggtaaaacc gggtgatcac caccggcgcg
     1081 gatggatacg ctcatcacgt cgaaccgcac cctcgacctg agagcgtatc
//
LOCUS       DAOTON010000940         1128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_103933_length_1128_cov_3.123020, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000940 DAOTON010000000
VERSION     DAOTON010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103933_length_1128_cov_3.123020"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1128)
                     /locus_tag="P5292_14060"
     CDS             complement(<1..>1128)
                     /locus_tag="P5292_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRS40300.1"
                     /translation="KQHWTSAKSFDELAEKMSTTLQEIPGISVGFQFPVQMRFNELMT
                     GGRQDVVCKIFGDNLDSLARYATLLGEEVRQVPGAVDLYVEAVTGLPQLVVRYRREAL
                     LSYGLRVDEVNQAVQTAFAGASAGMLFEDDRRFDLVVRLNDNARTDLQDLRELPVLLH
                     DGSPIPLSQLADVNLEVGPNQIQRENARRRITVGFNVRGRDVQSIVEELKERVRTKLD
                     LPSGYSITYGGQFENLIAAKARLAIAVPIALGLILLLLYFAFGSIRQSVLVFSAIPLS
                     AIGGIVALWLRGMPFSISAGIGFIALFGVAVLNGIVLVTEFNLLKKSGVNSIKERIVK
                     GTRIRLRPVLMTAAVASLGFLPMALSKGAGAEVQRPLATVV"
BASE COUNT          258 a          334 c          303 g          233 t
ORIGIN      
        1 atcaccacag tggcgagcgg gcgttgcacc tcggccccgg cacccttact gagcgccatc
       61 ggcagaaagc ccagcgaagc cacggcagcg gtcatcaaca caggacgcaa acggatccgg
      121 gtacctttca cgattctctc tttaatggaa tttaccccgg actttttcag gaggttgaac
      181 tcggtcacca aaacgattcc gttcagtacg gctacaccga agagggcgat aaagcctatc
      241 ccggccgaaa tgctgaaagg catcccgcgt agccagaggg cgacaatgcc gccgatcgct
      301 gataagggga tggcgctaaa gaccaaaaca ctttgccgga tcgatccgaa ggcgaagtag
      361 aggagcagca ggatcaggcc cagtgctatc ggaaccgcaa tggcgagtct ggccttggcg
      421 gcgatcagat tttcgaactg gccgccgtaa gtgatcgaat agccggaagg aagatccaat
      481 ttggttcgca cacgttcttt gagttcttcc acgatgcttt gaacatcccg cccgcgtaca
      541 ttgaatccta ccgttatgcg gcgtcgggca ttctcccgct ggatctgatt gggaccgacc
      601 tccaggttga catccgccaa ttgcgagagc gggatcggac ttccgtcatg caacaggacc
      661 ggtaattccc ggaggtcctg aaggtcggtc ctggcgttat cgttcagtcg aaccaccagg
      721 tcgaaccggc ggtcatcctc gaacaacatc ccggccgagg cacccgcaaa tgcggtctgc
      781 acggcctggt tcacttcatc cacccgaaga ccatacgaaa gcaaggcttc gcgccggtac
      841 cggacaacca gttgcggcaa cccggtaacg gcttcgacat acaggtccac tgcaccggga
      901 acttgtcgta cttcttctcc aagtagtgtt gcgtaacggg ccagcgagtc caggttgtcg
      961 ccgaagatct tacacaccac atcctgcctt ccaccggtca tcagttcgtt gaatcgcatc
     1021 tgtaccggga actgaaagcc gaccgaaatg cctggaatct cttgcagggt agtactcatc
     1081 ttctctgcca gttcatcgaa cgattttgcg cttgtccagt gttgcttg
//
LOCUS       DAOTON010000941         1124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_104578_length_1124_cov_2.333957, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000941 DAOTON010000000
VERSION     DAOTON010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104578_length_1124_cov_2.333957"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="P5292_14065"
     CDS             <1..652
                     /locus_tag="P5292_14065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40301.1"
                     /translation="NCTVVSVKVYTDTPGLREFQLLDGGGNVVNSMQVNLPADTTRVN
                     LNFSITPGTGYRLTTNASINQSTLGTTTPRLKRSSLGVAYPYTINNVISITGSNQGQN
                     YYYYFYDWEVQEPGLVCVSERTPVLADITTAVGELAANSGLSEFPNPATSFLNIRSEK
                     AGDVNVRIYDAAARLLRQDSFRGMSHQVSIEDLASGIYILHLEKEGAVYQYRLTVN"
     gene            complement(902..>1124)
                     /locus_tag="P5292_14070"
     CDS             complement(902..>1124)
                     /locus_tag="P5292_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRS40302.1"
                     /translation="EGNRKIVVTRNLKEYEEQLTPYGFFRVHHSHMVNLNHLVRYVKG
                     EGGYVVMTDGSSVDVSRRKKEEFLARLAG"
BASE COUNT          275 a          342 c          267 g          240 t
ORIGIN      
        1 caactgcacg gtcgtttccg ttaaagtgta caccgatact ccgggcctcc gcgaattcca
       61 gctcctcgat ggcggtggta acgtcgtcaa cagcatgcaa gtcaacctgc cggccgacac
      121 cacccgcgtg aacctcaatt tcagcatcac ccccggtacc ggctatcgcc tgaccacgaa
      181 cgcctccatc aaccaatcca ccctgggcac cactaccccg cgcctcaagc gcagctcgct
      241 gggtgtcgct tatccgtata ccattaacaa cgtgatcagc atcaccggtt ccaaccaggg
      301 ccagaattac tactactact tctacgactg ggaagttcag gaacccggac tggtttgcgt
      361 ttccgagcgc acgccggtat tggccgacat caccaccgcg gtcggcgaac tcgccgctaa
      421 cagcggactg agtgagttcc cgaacccggc tacgtccttc ctgaacattc gttcggaaaa
      481 ggccggtgac gtgaacgtcc gcatctacga cgctgccgcc cgactgctcc gtcaggattc
      541 cttccgcggc atgagccacc aggtctccat cgaagacctc gccagcggca tctacatcct
      601 ccatctcgaa aaagaaggag ccgtctatca gtaccgcctc acggtgaatt gacagtaatt
      661 caaagcatga aaaaagcgac tgaggttagc tcagtcgctt tttttgtaac tcgtagttag
      721 taattagtaa ttagcaattg gtaattggtg attggtgatt ggtgattggt gattggtgaa
      781 aagacaaaaa aaattctaat tgaatcacta ataacaaata acaaatcact aacaacccgg
      841 aacccgccaa catgccgtag ctttggcaaa ggcagagaac acggaacaac aaaccccctt
      901 tctaccctgc cagccgcgcg agaaactcct ccttcttcct gcgggagacg tcgaccgagc
      961 tgccgtcggt catgaccaca taaccgcctt cgcctttgac gtagcgcacc aggtggttga
     1021 ggttgaccat gtgcgagtgg tggacacgga aaaagccgta cggggtcagt tgctcttcat
     1081 actccttgag gttccgcgtc acgacgatct tgcgattgcc ctcg
//
LOCUS       DAOTON010000942         1122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_104841_length_1122_cov_4.362699, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000942 DAOTON010000000
VERSION     DAOTON010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104841_length_1122_cov_4.362699"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="P5292_14075"
     CDS             complement(<1..320)
                     /locus_tag="P5292_14075"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRS40303.1"
                     /translation="MTGTLPRVVLHPKKERSLLQRHPWVFSGAIRSIDQGVSEGDWVE
                     VIGSDGRFLGAGHFHEGSIKVRLLSFHPTVGEQQFWNERILAAYRLRERLGLTNSKQT
                     TAYRL"
     gene            complement(367..984)
                     /locus_tag="P5292_14080"
     CDS             complement(367..984)
                     /locus_tag="P5292_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRS40304.1"
                     /translation="MAIVNRKLSKDFYRRSDVVGIARDLLGQILVTRINGTPTAGYIT
                     ETEAYAGISDRASHAFGDRNTPRTRIMYLEGGVAYVYLIYGIHSLFNVVTNQEGIPHA
                     VLVRSLFPFKGIEVMTDRRGRQLSPDKLCTGPGSVSRALGIHFSDTGTDLLGDRIWIE
                     RNELKLPDTLIRQGPRIGVDYAGPDALLPYRFHVRHPDLKSLWKL"
BASE COUNT          262 a          314 c          296 g          250 t
ORIGIN      
        1 aggcggtacg cggttgtttg cttcgaattc gtcaaaccca gtcgctcccg caggcggtat
       61 gccgccagta tgcgttcatt ccaaaactgt tgttcaccaa cagtcggatg aaaagacagg
      121 aggcgaactt tgatagatcc ttcatggaaa tgcccggccc ctaggaaacg gccgtcggac
      181 ccgattacct ccacccaatc gccttccgaa accccctggt caatggaacg aatggcgcca
      241 ctgaagaccc agggatgccg ctgaagaagc gaacgttcct ttttaggatg caatacgacg
      301 cgtggcaggg ttcctgtcat cctgcaaaga aaccacttcc ggttagatag cccaagggct
      361 gcaggatcaa agcttccaca gggatttcag gtccgggtgc ctgacatgga aacggtaggg
      421 aagtaaggca tccggtccgg cataatccac tccgattcgg ggcccttgcc ggatcagcgt
      481 atcggggagc ttcaattcat ttcgctcgat ccagatccgg tctcccagca gatcggttcc
      541 ggtatcgcta aaatggatgc cgagcgcacg cgagacgctc cccggaccgg tacacagttt
      601 atccggcgac aattgccgtc ctcttcgatc ggtcatgact tcgattccct tgaacggaaa
      661 aagacttctc actaagacgg catgcggaat tccttcctgg ttcgtgacaa cattgaagag
      721 cgaatgaatc ccgtagatta gatagacata agcgactccg ccttcgaggt acatgattcg
      781 ggttctcggc gtgttgcgat caccgaatgc atgggaagcc cgatccgata ttccggcata
      841 ggcttctgtt tccgtaatgt atccggcagt aggcgttccg ttgatacggg taaccaggat
      901 ctgacccaac aagtcccggg caatgccaac gacgtcgctt cggcgataaa aatccttcga
      961 caacttccgg ttcacgatcg ccatgacagg agttgctcct ccgaaaggtg aagctgatcg
     1021 tgctcgatca tccaggtgat gaccttcagt cgctgctctt cgccggcatc acggatggaa
     1081 tccaccaatt cctggagagc catcggtcgc agtttgagtt gt
//
LOCUS       DAOTON010000943         1122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_104847_length_1122_cov_3.462043, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000943 DAOTON010000000
VERSION     DAOTON010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104847_length_1122_cov_3.462043"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="P5292_14085"
     CDS             complement(<1..316)
                     /locus_tag="P5292_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40305.1"
                     /translation="MKRPFPLFPVHILGSLLFLSLPILYMRTPLSELTWDEFLRSEMT
                     LRDLVAHILMLGFFYLSYYLLIPRLFTTRRYGIFITVVVASLVAVYVVPNLFYPYRPH
                     FHP"
     gene            complement(414..1004)
                     /gene="nadD"
                     /locus_tag="P5292_14090"
     CDS             complement(414..1004)
                     /gene="nadD"
                     /locus_tag="P5292_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRS40306.1"
                     /translation="MKIGLYFGSFNPVHNGHMIIAGYVSEFTDLDQVWLVVSPHNPLK
                     PKQTLLNDYQRLAMVKLAIGNNRKIKATDIEFKLPKPNYTIDTITYLHEQFPSHTFVL
                     IMGADNLESFKKWKNWEMLLEQCELYVYPRKESDGGELLHHPRVKVINAPLVELSASF
                     IRESIRNKKDVRYMLPESVWDYVEEMHFYEKGPSND"
BASE COUNT          263 a          284 c          320 g          255 t
ORIGIN      
        1 ccggatggaa gtggggacga taagggtaaa agaggttggg caccacataa accgcaacca
       61 gcgaggcaac gaccactgtg atgaaaatcc cgtaccggcg ggtggtgaac aggcgcggga
      121 ttaggaggta gtagctcagg taaaagaacc cgagcatcag gatgtgcgcg accaggtccc
      181 gcaaggtcat ctccgaccga aggaactcgt cccaggtcag ctcgctcagc ggcgtccgca
      241 tgtacaggat cggcagcgag agaaacagca ggctgcccag gatgtgtacc ggaaagagcg
      301 gaaacggacg cttcatacag gatactcaaa cgtacgattt cccgcgttag cagggggaat
      361 tcttcgggga aacggaagat tcctgcggca gaaccgggct gtacgggctg ctgtcagtcg
      421 ttcgacggcc ccttttcgta aaagtgcatt tcttccacgt agtcccagac ggactcggga
      481 agcatgtagc gaacgtcttt cttgttccgg atggattccc ggatgaaact ggcggaaagc
      541 tcgaccaacg gggcgttgat caccttcacg cggggatgat gcaacagctc gccaccgtcg
      601 ctctctttgc gcgggtaaac gtacaactca cattgttcca gcaacatttc ccagttcttc
      661 cacttcttaa aactttccag gttgtccgcg cccatgatca gtacgaacgt atgggaagga
      721 aactgctcgt gcaggtaggt gatggtgtcg atcgtatagt tcggcttcgg cagcttgaac
      781 tcgatgtcgg tggctttgat cttccggttg ttcccgatgg ccagcttcac catcgccaaa
      841 cgttggtagt cgttcaacag ggtctgcttc ggcttcaacg gattgtgcgg cgacaccacc
      901 agccacacct ggtccaggtc cgtaaactcc gacacatacc ccgcaatgat catgtgcccg
      961 ttatggaccg ggttaaagga gccgaagtag aggccgattt tcataaagtg ggaactggta
     1021 actggtaatt tgtaactagt aactagtaat tggtgatttg tgatttgtga ttggggttat
     1081 tatctcgcgg ctaaatcgct atgccttatt tttcgtccct cg
//
LOCUS       DAOTON010000944         1122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_104866_length_1122_cov_2.832240, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000944 DAOTON010000000
VERSION     DAOTON010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104866_length_1122_cov_2.832240"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="P5292_14095"
     CDS             <1..1014
                     /locus_tag="P5292_14095"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRS40307.1"
                     /translation="ELMVVLLFLLAIWYHQLLGYGWMQLRGQLSIVWNARSVDEVLAD
                     PTTPDSVKAQLTYIHAVRRFAIDSLGLRDTRNYTTYYDQRGQPLLWVLTASEVFRIRP
                     YQWKFPVVGTVSYKGFFRKEVGEREAQLLEESGFDIDYGEVGAWSTLGWFRDPILSGM
                     LRRGQGRLAELIIHEMTHATLYVPGEVDFNENLASFIGEEGAKRFLNAHFGAEAPETI
                     RYLDFLHDYNALARHLVRGTEWLDSVYQTREFYKWGIAEKQLLKRDMIDSVLSALDTV
                     TFHDTLRAKRRFAGSRPNNAYFINYIRYDAMKDSLDRVFRTQFGSDFAAFLDFQQKRY
                     AVQ"
BASE COUNT          260 a          248 c          330 g          284 t
ORIGIN      
        1 gagctgatgg tcgttttact ttttttgctg gctatctggt atcaccaact gttgggctac
       61 ggatggatgc agttgcgcgg tcagttgtcc atcgtgtgga atgcgcgtag tgttgatgaa
      121 gtgttggcag atccgacaac acctgacagc gtcaaagctc agttgactta tatccatgca
      181 gtcagacgct ttgccattga ctcgctggga ttgcgcgata ccaggaacta taccacctac
      241 tacgaccagc gcggtcagcc gttgttgtgg gtcctgacgg ccagtgaagt ctttcgtatc
      301 cggccttacc aatggaaatt cccggtagtg ggtacggtgt cctataaagg gttctttcga
      361 aaggaagtcg gcgagcgtga agcgcagctg ttggaggaga gcgggttcga catcgactac
      421 ggcgaagtgg gagcctggag cacgttgggg tggttcaggg atccgatcct ctccggaatg
      481 ctgaggcgcg gacaaggaag attggcggag ctgatcattc atgaaatgac gcatgccacc
      541 ctgtatgttc cgggcgaggt cgatttcaat gagaacctgg cgagtttcat tggcgaagaa
      601 ggggcgaagc gatttctaaa cgcccacttc ggcgcggagg cgccagagac cattcggtat
      661 cttgatttcc tgcatgatta caatgcactg gcccgtcacc tggtgcgtgg taccgaatgg
      721 ctggattcag tttatcagac ccgagaattt tacaaatggg gaatcgcgga aaagcagttg
      781 ttgaaacgcg acatgatcga ttcggtgctg agtgcattgg ataccgtgac ctttcatgat
      841 acgctccggg ctaaacggcg gtttgccggt tctcgtccga acaatgccta ctttataaat
      901 tatatccggt atgatgcgat gaaagattcg ctggatcgcg tatttcggac gcaattcggg
      961 agtgattttg ctgcctttct ggattttcaa cagaaacgat acgctgtaca atgaagtctt
     1021 ccgtttcaat aaataaatcc aaaggagtat cgggggaagg gcttattgac cgattactag
     1081 aagaacgtca ttcgttgcag caaacggagc ggatcgcgcg gg
//
LOCUS       DAOTON010000945         1122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_104912_length_1122_cov_2.124649, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000945 DAOTON010000000
VERSION     DAOTON010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104912_length_1122_cov_2.124649"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="P5292_14100"
     CDS             <1..1059
                     /locus_tag="P5292_14100"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40308.1"
                     /translation="VGGTYSGPGVSNGSFDPAVAGVGNSVIAYSFTDANGCSASASTS
                     ITVQDCNNGGGGCTAPPSKPRYLIGPRIISCGQTGIVYSVPTDTAASSYVWSVPTGAV
                     IVADNGNSITVDFPAGAQTGDICVTAVNACGSSDPLCRTILVGGLYTRRIRGPQEVCR
                     TDSSVTYYIPQVAGAVSYNWTIGGNAGMTVSGNNVTINFSSCTLNWVVLRVEVTNACG
                     DVRSRSLWIHVRDCNGGGHDDDDDDDDDDDGDGHHGDDDRLLSPFANVTASLELQVFP
                     NPSAGLFMVAVPEGNAGTVRVTVVDLSGKELYRAEQTLDAGSQQLQIDGNAFAPGVYF
                     LRVEQDGAVGNERFIIAR"
BASE COUNT          222 a          280 c          363 g          257 t
ORIGIN      
        1 gttggaggaa cttatagcgg ccccggcgtg agtaacggct cctttgatcc ggcagtggcc
       61 ggggtaggga attccgtgat cgcatacagt tttaccgatg cgaacggatg ctccgcatcg
      121 gccagcacct cgatcacggt tcaggattgc aacaatggag gaggcgggtg tactgctccg
      181 ccaagcaaac cccgttacct gatcggccct cggatcatat cgtgcggtca gaccgggatc
      241 gtgtattcgg ttccgacgga tacggctgca agctcgtatg tgtggtcagt gccgacggga
      301 gcagtgatcg tagctgacaa cgggaactcg atcacggtgg atttcccggc cggcgcccaa
      361 acgggtgata tctgtgttac agccgtcaat gcctgcggca gcagcgatcc tttgtgtcgc
      421 acgattctgg tcggcggact gtatacacgc aggatccggg gtccgcagga agtttgccgg
      481 acggatagtt cggtcaccta ttacattccg caagtggcgg gtgccgtttc gtataactgg
      541 accatcggcg gaaatgccgg tatgacggtc agcggcaaca acgtgacgat caacttcagc
      601 agttgcacac tcaactgggt ggtgcttcgc gttgaagtca ccaatgcctg cggggatgtg
      661 cgcagccggt ccttgtggat ccacgtacgt gactgcaatg gcggcggcca tgatgacgat
      721 gacgacgatg atgacgatga tgatggtgac gggcatcatg gcgacgatga tcgtttgctg
      781 agtccgttcg cgaacgtcac cgctagtctg gaattgcagg tgttcccaaa tccttcggcg
      841 ggtctcttca tggtggcagt accggaaggt aacgccggaa cggttcgggt gactgtagtc
      901 gatctgagtg gtaaggagct ctaccgtgcg gagcaaacgc ttgatgcagg atcgcagcag
      961 ttgcagatcg acggtaatgc ttttgcgccg ggtgtttatt tcctgcgcgt tgagcaagac
     1021 ggcgcggtag gcaacgagcg cttcattatc gcacgttaag tactttgttc ctgttgatgt
     1081 tagcggcctg cccggaaggg tgggccgttt ttattaggga cg
//
LOCUS       DAOTON010000946         1121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_104994_length_1121_cov_3.293621, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000946 DAOTON010000000
VERSION     DAOTON010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104994_length_1121_cov_3.293621"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..>1121)
                     /locus_tag="P5292_14105"
     CDS             complement(177..>1121)
                     /locus_tag="P5292_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HRS40309.1"
                     /translation="DGTIRSLAQFRIGTILDYSVEGKESEVDLDHGLEQTLATIRRAK
                     GDPHIPFSVFKPSGFARFALLEKLNAGQTLSEAEQLEFTRFRKRVETICELGAANGVP
                     IFVDAEESWIQDVVDQLVREMMMRFNKERVMIFNTLQMYRHDRIDYLRQSLEHAKVNG
                     YKAGFKLVRGAYMEKERERAEKMGYPSPIQPDKTATDRDYDAALRLCMERIEDVAICA
                     GTHNEASSLLLTELMQSKGLATDHPHVWFSQLYGMSDHISFNLAKAGYNVCKYVPYGP
                     LTAVLPYLIRRAQENTSVAGQTGRELSLIIQERKRRRT"
BASE COUNT          242 a          291 c          300 g          288 t
ORIGIN      
        1 ctgatcaatg ccggcgaagt tggaggcctg gatgaattac tgtggaaaga caaactcgtt
       61 cgacggggag tgtatatgtt ccgcggatcg atcaccaatc gctacgttgc tgatgcgtcc
      121 aagctgccgt acaaggatct cgacctgtta caggctgccc gaatctgatc gccgcatcag
      181 gttcttctgc gtttccgttc ctggatgatg agtgaaagtt cccggcctgt ttgtccggca
      241 accgaagtgt tttcctgcgc tctgcgaatg aggtagggaa gtacagcggt caatggtccg
      301 tatggaacgt atttgcaaac gttgtagccg gctttcgcaa gattaaagct gatgtggtcg
      361 ctcattccgt aaagttgaga gaaccagacg tgcggatgat cagtggccag gcctttcgac
      421 tgcatgagtt cagtcaacaa caagctgctg gcttcgttgt gcgttccggc gcaaatcgcc
      481 acatcctcga tgcgttccat gcacaggcga agcgcggcat cataatcacg gtcggttgcg
      541 gtcttgtcgg gttggatcgg agaggggtac cccatttttt cggcacgttc ccgttccttt
      601 tccatatacg cgccacgtac cagtttgaag ccggctttat atccattcac tttggcgtgt
      661 tcaagcgatt gacggagata gtcgatcctg tcgtgccggt acatctggag ggtattgaag
      721 atcatgacac gctccttatt gaagcgcatc atcatttccc gtaccagttg gtccacaaca
      781 tcctggatcc aactctcttc ggcatcaacg aaaatgggta caccgttcgc tgctcccagt
      841 tcacagatcg tttccacacg tttccggaaa cgcgtgaatt ccagctgctc cgcttcggag
      901 agtgtctgtc ccgcattcaa cttttcaagt aaggcgaacc gggcaaagcc gctgggcttg
      961 aaaaccgaga aaggaatatg gggatcacct ttcgcgcgac ggatcgtagc cagcgtttgt
     1021 tctagaccgt ggtctaaatc cacctcgctt tctttacctt ccacagaata gtcgagaatg
     1081 gtgccgatcc tgaattgtgc cagggagcgg atggtaccgt c
//
LOCUS       DAOTON010000947         1116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_105813_length_1116_cov_1.954760, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000947 DAOTON010000000
VERSION     DAOTON010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105813_length_1116_cov_1.954760"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="P5292_14110"
     CDS             complement(<1..512)
                     /locus_tag="P5292_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRS40310.1"
                     /translation="MSSFEFLLLFASILASGSLIFLIKRIPPNALKLLLSFSGAYLFA
                     LSLLHLLPEVYSNGGAGIGIFILGGFFLQLLLEIFSEGIEHGHIHVHKHDHKHAFPLG
                     VILGLCIHSFLEGMPLVQHFTDANTQRSLLAGIILHHIPVALALTSMLLASHISKPAT
                     FFYLLLFASMA"
     gene            complement(558..>1116)
                     /locus_tag="P5292_14115"
     CDS             complement(558..>1116)
                     /locus_tag="P5292_14115"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRS40311.1"
                     /translation="LNKRGFDVCGFDLSKASIAHNRQFENDRLHFFVYDMRDVFRVNY
                     FDYVLNLFSSFGYFESERENYNAFRANAVACKPGGSIIVDFFNAKKVCAAGGNTVEQE
                     IDGITFTSRKYFTEHHVRKEVRFFAGGEERNFTEQVRLFNREDFHRFAQKAGLQVLQE
                     YGDYQLNPFDEAKSDRYILVAGKPA"
BASE COUNT          266 a          277 c          337 g          236 t
ORIGIN      
        1 gccatggaag cgaacagcaa caggtagaag aacgtggccg gcttgctgat gtgggaggcg
       61 agcagcatgc tggtcagggc cagggctacc gggatatgat gcaggatgat accggccagt
      121 agggagcgct gggtgttggc atcggtgaaa tgctggacca ggggcatgcc ttccaggaac
      181 gagtgaatgc acaatccgag gatgacgcct aacgggaacg cgtgcttgtg atcgtgtttg
      241 tggacgtgga tgtgaccgtg ctcgatccct tccgagaaga tctccaataa gagttggaga
      301 aagaaacctc caaggatgaa gatgccgatg ccggcgccgc cgttactgta aacctccggc
      361 aggaggtgga gcaggctgag ggcgaacagg taggcgccac tgaacgacag cagtagtttg
      421 agtgcgttcg gaggaatcct cttgatcagg aagatcaggc ttccactggc caggatggag
      481 gcaaagagca gtaggaattc gaaggatgac atgccgggcc gacgaagtta cgattccgcc
      541 gcttcttgaa aaagccgtca ggcaggcttg ccggcgacca ggatgtaacg gtcggacttc
      601 gcttcgtcga agggattcaa ctgataatct ccgtattcct gcagaacctg caagccggcc
      661 ttttgcgcga agcggtgaaa gtcttcccga ttgaaaagcc gtacctgctc ggtaaaattc
      721 cgttcctctc cgcctgcgaa gaaacgcact tccttccgca catggtgctc cgtaaagtac
      781 ttcctgctgg tgaaggtgat gccgtcgatt tcctgctcaa cggtgttccc accggcggcg
      841 cagacttttt tggcattgaa gaaatccacg ataatgctgc cgccgggttt acaggcgacc
      901 gcgttcgccc ggaaggcgtt gtagttctcc cgttcgcttt cgaagtatcc gaaactgctg
      961 aacaggttca acacgtaatc gaaatagttc acccgaaaga catcgcgcat gtcgtacacg
     1021 aaaaagtgca aacggtcgtt ttcgaactgg cgattgtgcg caatgctggc tttggaaagg
     1081 tcgaagccgc atacatcaaa tcctcgtttg ttcagg
//
LOCUS       DAOTON010000948         1111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_106620_length_1111_cov_1.785038, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000948 DAOTON010000000
VERSION     DAOTON010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106620_length_1111_cov_1.785038"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..>1111
                     /locus_tag="P5292_14120"
     CDS             192..>1111
                     /locus_tag="P5292_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRS40312.1"
                     /translation="MPITQEQVVDALRNVIEPDLKKDLITLNMVEDIVVDGKNVSFTV
                     VLTTPACPLKALIQSACVNAIVHFVDAEAKVTVNMTARTTSGRSSSDTQLPNVRNIIA
                     VASGKGGVGKSTVAANLAVALAKKGARVGLIDADIYGPSQHIMFGVENEQLYVNERDG
                     KQFMIPVDKYGVKMLSIGLMVDPNQPIPWRGPMASKALKQLFSDAEWGELDYLLIDLP
                     PGTGDIHLTLVSAVPVTGSVIVSTPQHVALADARKGIGMFRLDTINVPVLGLIENMAY
                     FTPAELPQNKYYIFGKEGCRKLAEELKVPFL"
BASE COUNT          262 a          313 c          289 g          247 t
ORIGIN      
        1 agtcgaactt tccctctgaa gaggtgattg ttccgagttc cgggttccga gttgaacatt
       61 tgattcttcc aacccgctcc agtatatagg cctgacattc agttatatac aatattaccg
      121 tacccggtaa ccaacccgga acccggaacc cggaacacga aactccaaac cctaataggt
      181 atctttgccc catgccaatt acccaagaac aggtcgtaga cgcgctgcgg aatgtgatcg
      241 agccggacct gaaaaaagac ctgattaccc tgaatatggt cgaggatatc gtggtggacg
      301 gtaagaacgt ctccttcacg gtcgtcttaa ccacccctgc ttgcccgtta aaggcgctca
      361 ttcaatcggc ctgcgtcaat gcgatcgttc atttcgttga tgccgaagct aaagtgaccg
      421 tgaacatgac ggcccgcacg acgagtggcc gcagctcttc cgatactcag ttacccaatg
      481 tccgcaatat catcgctgtt gcttccggca aaggtggcgt cggtaaatcg accgttgccg
      541 ccaatctcgc tgtcgccctc gccaagaagg gtgcgcgggt cggtttgatc gatgccgata
      601 tctacggtcc ttcccagcac atcatgttcg gggtggagaa cgagcagctt tacgtgaacg
      661 agcgcgacgg aaagcaattc atgatcccgg tcgacaaata cggcgtcaag atgctttcga
      721 tcggactgat ggtcgatccg aaccagccga ttccgtggcg tggtccgatg gcctccaaag
      781 cgctgaagca attgttcagc gacgcggaat ggggcgagtt ggattacctc ctgatcgacc
      841 ttcctcccgg aaccggcgat atccacctca cactcgtgtc tgcagtaccg gtgaccggct
      901 cggtcatcgt cagtacgccg caacacgttg ccctggcgga tgcccgaaaa ggtatcggaa
      961 tgttccgcct cgatacgatc aatgttccgg tactcgggtt gatcgagaac atggcatatt
     1021 tcacgccggc ggagttgccg caaaacaagt actacatctt cggaaaagaa ggctgcagaa
     1081 agttggccga agaactgaag gtcccgttcc t
//
LOCUS       DAOTON010000949         1106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_107353_length_1106_cov_2.658421, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000949 DAOTON010000000
VERSION     DAOTON010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107353_length_1106_cov_2.658421"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1106)
                     /locus_tag="P5292_14125"
     CDS             complement(<1..>1106)
                     /locus_tag="P5292_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40313.1"
                     /translation="WDALGSTPFLQGVDGSFTINDKAYVILHNSNEVWEFDPHGVPDV
                     NSAVCDTLYYSNGNQNWFAQLIRTSDGNLVTVGTENQIYNWANGDIVLSKYDTAFNLI
                     WSRKFYVGGGMDIANGVLQTSDGGLLIHKAFGNTNSPGSFSSGYILKTDSAGNLEWEQ
                     TLMGQSSGDNYSSAAVENSQGEFIIYGHVQDHPGCNGYATRMSKLSATGSILWSNCFP
                     LNPDRTGGIVKLPFSDTYISAYNDQSTGAVELRSWDDSGNQTGLINYQYGSQFNSAGS
                     VMPCPSGGFFLAGQFDRGNGNRNAFVTKFDDAMNMQWESTDSVYNENYFYNVFIDANG
                     TVYCTGGTNVTGQPGDLIMSVFDGSGQFIRHELY"
BASE COUNT          304 a          284 c          216 g          302 t
ORIGIN      
        1 gtagagctca tgcctgataa attgtccgga accatcaaat accgacatga tcaaatcacc
       61 cggttgtccc gttacgttag tacctcctgt acaataaact gttccgttag catcaataaa
      121 aacattataa aagtaattct cattgtatac gctgtctgtt gactcccatt gcatattcat
      181 tgcatcatcg aactttgtca caaatgcgtt ccggttccca tttccacgat caaactggcc
      241 ggctaagaaa aatccgcccg aaggacaagg catgacgctt ccggcagaat tgaactggct
      301 accatattgg tagtttatca aacccgtttg gtttccagaa tcgtcccatg aacgcagttc
      361 cactgcacct gttgactgat cattgtatgc tgaaatatag gtgtctgaaa acggcaactt
      421 cacaatccct ccggtccgat ccggattcaa cggaaaacaa tttgaccaaa ggattgaccc
      481 ggttgcggac aatttcgaca tcctcgttgc atatccgttg catcctggat ggtcttgaac
      541 atgtccatat atgataaatt caccttgcga attttccacc gctgcagaac tataattgtc
      601 acccgaactc tggcccatca gggtttgctc ccattccaaa ttaccagccg aatccgtctt
      661 caggatgtaa cccgatgaga agctgcccgg cgaattggta ttcccaaaag ccttatggat
      721 aagtaatcct ccatcactgg tttgaagaac cccgttcgcg atatccatac caccacccac
      781 atagaacttc ctggaccaga tcaaattgaa tgcggtatcg tatttactca gtacgatatc
      841 cccgtttgcc caattgtaaa tctgattctc tgtccctaca gtaacaaggt tgccatcgga
      901 ggtccggatg agttgagcaa accaattctg atttccattg ctgtaataca gggtgtcaca
      961 tacagcggaa ttgacatctg gaacgccgtg aggatcgaat tcccacactt catttgaatt
     1021 atgtaagatg acatacgctt tatcgttgat cgtaaatgat ccgtccactc cctggagaaa
     1081 tggagttgag ccgagtgcat cccatt
//
LOCUS       DAOTON010000950         1106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_107378_length_1106_cov_2.268316, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000950 DAOTON010000000
VERSION     DAOTON010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107378_length_1106_cov_2.268316"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1106
                     /locus_tag="P5292_14130"
     CDS             <1..>1106
                     /locus_tag="P5292_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40314.1"
                     /translation="ILRKNFQWLILLLTVSLLTAFLYLRYTHPVYQANSTLKIGMVNT
                     ANAVLNQPGSQMYELINGSNNAFAGDVELMRSREMVKRIIEKLPLEVTYYLRGAILDN
                     ELYEQCPFSIEYELLDSSIYGVLINVDFLPTGEYRFEYEHLGKTVSSTGRPGEWKSTP
                     EFKMRLQIVDSLSIQKHNKRIKDYPYYFCFNTTADLTEEYYENYSVTIVNPSAQTVRV
                     TFKDQNDRKAADFVNMAISEFNASEQERKSEGSIRSLAFIDETIASIDSELKISEVSL
                     ELFKTRNKIINPNENAHDALEHINQMVNDRVKYQLGLAVMMRLEKNLNTLEDIENLVP
                     LMAGDYEDDVIKKLLDRVQELQDKKASLRFMATD"
BASE COUNT          304 a          269 c          272 g          261 t
ORIGIN      
        1 cattctccgg aagaattttc aatggctgat tctgcttctt acggtcagtt tgctgacagc
       61 ctttctctac ctccgctaca cgcatccggt ttatcaggct aattccaccc ttaagatcgg
      121 catggtcaat accgcgaatg cggtactcaa tcagccgggg tctcagatgt atgaactgat
      181 caacggttcg aacaacgcgt tcgcgggcga cgttgaattg atgcgttcga gggagatggt
      241 gaagcggatc atcgaaaaac ttccgttgga ggtcacctac tatttgcgcg gagctatcct
      301 ggacaatgag ctttacgagc aatgtccgtt cagtatcgaa tacgagctgc tggattcgag
      361 catctatggc gtacttatca atgtcgactt tcttccaacc ggcgagtacc ggtttgaata
      421 tgaacacctg ggtaaaactg tttcctcgac cggtaggccg ggggaatgga agtcgacacc
      481 cgaattcaag atgcggctgc agatcgtcga cagcttatcc atccagaagc acaacaagcg
      541 tatcaaggac tatccctact atttctgttt caacactacc gccgacctta cggaggaata
      601 ttacgaaaac tactccgtca cgattgtcaa tccttccgct caaacggtca gggttacctt
      661 caaggatcag aacgaccgaa aagcggcgga cttcgtaaac atggcgatct ccgaattcaa
      721 cgcttctgaa caagagcgaa aatcggaagg ttcgatccgt tcacttgcat ttattgacga
      781 gactatcgca tcgatcgaca gcgaattgaa gatctcggaa gtttcgctgg agttgttcaa
      841 gacacggaat aagatcatca atccgaacga aaatgcgcat gacgcattgg aacacatcaa
      901 ccagatggtc aacgaccggg tgaagtacca gttgggcctg gcagtgatga tgcgacttga
      961 aaagaaccta aacaccctgg aagatataga gaaccttgtt cctctcatgg caggcgatta
     1021 cgaagacgat gtgatcaaga aactgcttga ccgggtgcag gaacttcagg acaagaaagc
     1081 cagtctgcgt ttcatggcga cagatg
//
LOCUS       DAOTON010000951         1104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_107668_length_1104_cov_3.192564, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000951 DAOTON010000000
VERSION     DAOTON010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107668_length_1104_cov_3.192564"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..>620
                     /locus_tag="P5292_14135"
     CDS             185..>620
                     /locus_tag="P5292_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40315.1"
                     /translation="MKPTRFTALVCLVAALALLLNTGCKKDSNSESENAMTALQEIGQ
                     AENINADMDHLDAEAAMTSGVSNQRTSETGRFGFGSCASILRDSINHTVTIDFGTGCT
                     GQDGRTRSGQILITYSGRYFDPGATWTVVFSDYHVDGRLIEGS"
     assembly_gap    621..630
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <631..1021
                     /locus_tag="P5292_14140"
     CDS             <631..1021
                     /locus_tag="P5292_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40316.1"
                     /translation="VTNNGFNAAGNMVWNIDAQNMRVTLPDGRWKTWNSQRTREMIDG
                     FGDSTCVNDVYRVNGTATGSNDRGDTFQATLTDLVREHDCRWITSGTISVSSSNRPDR
                     SLDFGSGACDDEATVTANGQSRIVHLR"
BASE COUNT          257 a          305 c          296 g          236 t
ORIGIN      
        1 acgcgcaccg actctatctg aaaaagggat tcaatattac ttccttccac ttcgccacca
       61 actgggggtg acttttttcg ctcgtcctta aacctcaagg cctaccggcg gtcatagggt
      121 tgtcgtttgc aaacacccct gcgttcggga cgaacgttac cccaacacct tcattcctct
      181 tactatgaaa ccaacacgtt tcacggccct ggtgtgtctt gtcgccgccc tggccctcct
      241 cttgaacacc ggttgcaaga aagacagtaa ttcagaaagc gaaaacgcga tgaccgctct
      301 ccaggagatc ggtcaggcgg aaaacatcaa cgcggacatg gatcatctgg atgccgaagc
      361 ggcgatgacc agcggtgtat ccaaccaacg cacctccgaa accggtcgct tcggcttcgg
      421 aagttgcgcc agcatcctgc gtgattcgat caaccacacg gtcacgatcg atttcgggac
      481 cggttgtacc ggacaggacg gaaggacgcg cagcggtcag atcctgatca cttattcggg
      541 tcgctacttc gatccgggcg ctacgtggac cgtcgtattt tccgactatc atgtagacgg
      601 gcggctcatc gaaggctcgc nnnnnnnnnn ggtgacgaat aacggtttca atgccgctgg
      661 taacatggtg tggaacatcg atgcgcaaaa catgcgggtt accttgcccg atgggcggtg
      721 gaaaacctgg aacagccaac gcacacggga gatgatcgac ggattcggtg acagtacctg
      781 cgtgaatgat gtctatcgtg taaatggtac cgctaccggc agtaatgatc gcggggatac
      841 atttcaagcg acccttaccg acctggttcg ggagcatgat tgccggtgga tcacgagcgg
      901 aacgatcagc gtgagcagtt ccaaccgtcc agaccgaagc ctcgatttcg gaagcggagc
      961 ctgcgacgac gaagcaacgg tcaccgcaaa cgggcagtcg cgtatcgtac acctgcgctg
     1021 aacttcagca gcatgaaaaa gccccgaagc agatgtttcg gggctttttt attttcttga
     1081 catttcagtt gatgccggga tcct
//
LOCUS       DAOTON010000952         1097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_108812_length_1097_cov_4.086372, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000952 DAOTON010000000
VERSION     DAOTON010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108812_length_1097_cov_4.086372"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="P5292_14145"
     CDS             complement(<1..543)
                     /locus_tag="P5292_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40317.1"
                     /translation="MSRITILRIVCLLALSTGSLITRAQVTPGFRSMVEAVLVDTSNR
                     YWDYPKPIAVTDPVLALMQDEFNRSDSLSLLFVEVSPANFRHLMQGEEVIDYCSRHDL
                     VATLTALAVHCNPDVRIKALQARLTRARIGLLINDKKLRNGRRVEENRVSGRFFLEIM
                     ERTPHDIPGSENATIHDLYMR"
     gene            745..>1097
                     /locus_tag="P5292_14150"
     CDS             745..>1097
                     /locus_tag="P5292_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRS40318.1"
                     /translation="MRFTILLLLLLIKLPVNGQALYFPPLTGTNWDTISPASLGWCTQ
                     YLDTIADFNAQRGSKAFLILKDGKIAVEQYYGTFTRDSLWYWASAGKTLTSFLTGIAQ
                     EEGYLDITRPTSDYLG"
BASE COUNT          281 a          310 c          246 g          260 t
ORIGIN      
        1 ccgcatgtac agatcgtgaa tggtggcgtt ctcactgccg ggaatatcat gcggcgtacg
       61 ctccatgatc tccaggaaga agcggccact gaccctgttc tcttccacgc gccgaccatt
      121 cctcagtttt ttatcattga tcaacaaacc gattctggcc cgggtcaacc gtgcctggag
      181 cgccttaata cgaacgtcgg ggttgcaatg gaccgccagc gccgtcaacg tagcgaccag
      241 atcatgccgt gaacaataat cgatcacctc ttcgccctgc atcaggtgcc tgaaattcgc
      301 aggagatact tcaacaaata aaagagatag ggaatcggaa cgattgaatt catcctgcat
      361 caacgctaag accggatcgg tgacggcaat aggcttcgga taatcccaat aacggttgct
      421 cgtatcgacc agtaccgctt ccaccatgct cctgaacccg ggagtaactt gcgcgcgtgt
      481 tatcagcgaa ccggttgata gcgccagcaa gcagacaatc cgaagtatcg tgatccttga
      541 catccgttga ataaattaaa ggaattaaac cactggtctg ctatagcgtc caatatattc
      601 gtgtcttttg tacaaacttc gtgcctgctc aagctgttaa ggcatatttc cgcctgcaac
      661 tgaaaaatgc caccattgcc tgagagctac tccacgaaaa acaatattga tcgtaaaact
      721 tccgaaccag catcctgcga taccatgcgc ttcacgatcc tgttactact tcttctgatc
      781 aagcttccgg taaacggtca agccttgtat ttcccgccgc tgacgggaac aaactgggac
      841 accatttctc ccgcttcgct cggctggtgt acgcagtacc tcgatacgat cgcggacttc
      901 aacgctcaac gaggcagcaa agcttttctc attctcaaag acggtaagat cgccgtagaa
      961 cagtactatg gaacctttac ccgcgatagc ctttggtact gggcatctgc cggaaaaacc
     1021 cttacctctt tcctgacggg tattgcacaa gaggaaggtt atctcgacat cacccgcccg
     1081 acgtccgatt acctggg
//
LOCUS       DAOTON010000953         1096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_109031_length_1096_cov_2.196926, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000953 DAOTON010000000
VERSION     DAOTON010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109031_length_1096_cov_2.196926"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="P5292_14155"
     CDS             <1..676
                     /locus_tag="P5292_14155"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRS40319.1"
                     /translation="VALSNISERTAAVILEPIQAEAGVRVPDPGYLQAVRKRCDETGT
                     LLLLDEIQTGMGRTGTLFRFEAEGIVPDVLILGKAFGAGMPLAAFLASPQLMRTLAHD
                     PVLGHITTFGGHPLSCAAALAGLQVTREDQLHQRARVAGEQFMKRLKHSRILQIRGEG
                     LLLALDLGSETLVQRLIPELAAHGIISDWFLFAPDCLRIAPPLILDESGIERACTTIL
                     DCLDAL"
     gene            686..>1096
                     /locus_tag="P5292_14160"
     CDS             686..>1096
                     /locus_tag="P5292_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS40320.1"
                     /translation="MNVLIVEDERQLSHEIEIFLSKQGYHCDVAFSGKSASEKIFVNS
                     YDFILLDVGLPDTSGFQLLKEAKEGGKDAAFILITARGSIDDKITGLDLGADDYLAKP
                     FSLLELQARMQAILRRKHGLKNNTIPIHDFVMDIQ"
BASE COUNT          244 a          315 c          286 g          251 t
ORIGIN      
        1 ggtcgcgctg tcgaacatca gtgaacggac cgcagccgtc atccttgaac ccatccaggc
       61 tgaagccggg gttcgtgtcc ctgacccggg ctatctgcaa gctgtccgaa aacgttgtga
      121 cgagaccggc accctgctcc tgctcgacga gatccaaacc ggcatgggac gaaccggaac
      181 tctcttccgg tttgaagcgg aaggaattgt tccggatgtc ctgatcctgg gtaaagcgtt
      241 cggagcgggc atgccgttgg ccgcctttct cgcttcaccg caactcatgc gaacactggc
      301 gcatgatccg gtactggggc acatcaccac gtttggtggt catccgcttt cctgcgctgc
      361 cgcgctggcg ggattgcaag tcacgcgcga ggatcaactc caccagcgcg cccgtgttgc
      421 cggtgaacag ttcatgaagc ggttaaaaca ttcacgtatt cttcaaattc gaggcgaggg
      481 attattgtta gctttggatc tcggcagcga aaccctggta cagcgtctca ttccggaact
      541 tgccgcacat ggaatcatat ccgattggtt cctgtttgct ccggattgtc ttcggatcgc
      601 tcctccgctc atcctggatg aaagcggcat cgagcgggcc tgtaccacga tcctcgactg
      661 tcttgacgca ctctgaacac ttacaatgaa cgtactgatc gtagaagacg aacgccagct
      721 ttcacacgag atcgagatct tcctttccaa gcaaggatat cattgcgatg tggcattcag
      781 cggcaaaagc gcatcggaaa aaatcttcgt caactcgtat gacttcatcc tgctcgatgt
      841 cggactgccg gataccagcg gttttcagct tctaaaagaa gccaaggaag gcggaaaaga
      901 tgccgctttc attctcatca ccgcccgcgg ttccatcgac gacaagatca ccggacttga
      961 ccttggtgcc gatgattatc tggctaagcc tttttcgttg ctcgaattgc aggcccggat
     1021 gcaggcgatc ctgcgcagaa agcacggact taagaacaac accatcccga tccacgattt
     1081 cgtgatggac atccag
//
LOCUS       DAOTON010000954         1095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_109134_length_1095_cov_3.573077, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000954 DAOTON010000000
VERSION     DAOTON010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109134_length_1095_cov_3.573077"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..>1095
                     /locus_tag="P5292_14165"
     CDS             355..>1095
                     /locus_tag="P5292_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40321.1"
                     /translation="MKELVRRLILLITIAATASLMPKKASATHLVGSDISYTCLGGNT
                     YRIDLTFYRDCRGSAAPLGVGIEFRSASCNQYFTDTLLLVTGTGNEITYPCPTLVTSC
                     DDPASTIPGIQEYQYSGIITFPMQCADWVISWSYCCRNCDITTMLVASPCLEGTNPGM
                     YIAATLDNLNLSCNSSPRFTNIPVAFLCVGQNFTYNHGVIDPDGDSLVYSLVNPLINA
                     TDSIPFLPGYSATNPITSSPAFAINSATG"
BASE COUNT          262 a          305 c          255 g          273 t
ORIGIN      
        1 ccctggcgat cgactatgcg atcaagcatg caaagaaggg gtctttcatt accatctgca
       61 gtgatgttgt gccggacgca ttggatcaga tcatgaagta caaggaagaa gaggatcact
      121 tcgagatcca caaggaggat attccgaacg cggtttccta agcagccaac ttacacttgg
      181 atttaaccca cccttaataa gaagggtggg tttttttatg attttaaagc attaaacaag
      241 cttgcgcatg gaacttttta gctgaactca agtaaaaatg cgttaagtct tacagtaccc
      301 ggaagcgggt gggggagcga atgccctcca ccgatccgac ccgaaagcaa ttccatgaaa
      361 gaacttgtcc gccgcctgat cctgttgatc accattgccg caacggccag tctgatgccg
      421 aaaaaggcct cggcaacgca cctggtcggt tccgacatct cctatacctg tctgggaggc
      481 aacacctacc ggatcgacct gaccttttac cgtgattgtc gtggatcggc agcaccgctc
      541 ggagtgggta tcgaattccg ctcggcaagt tgtaatcagt atttcaccga taccttgttg
      601 ctggtcaccg gcaccggtaa cgaaatcacc tatccgtgtc cgacccttgt cacgagttgt
      661 gacgatcccg cttccaccat tcccggaatc caggagtatc agtacagcgg catcatcacc
      721 ttcccgatgc agtgcgccga ttgggtgatc agttggtcct actgctgcag gaactgcgac
      781 attacaacaa tgttggtagc gtcaccttgt ttggaaggaa ccaatcccgg catgtacatc
      841 gccgcgacgc tcgataacct gaaccttagt tgcaattctt ctccgcgctt taccaacatt
      901 cctgtagctt ttctatgtgt cggacagaat ttcacttata accacggggt gatcgatccc
      961 gatggcgatt cgttggttta cagcctggtg aaccccctca tcaacgctac cgactccatt
     1021 ccatttttac ccggctactc ggctacgaat ccgatcacgt cttctcctgc atttgccatc
     1081 aacagcgcca ccggt
//
LOCUS       DAOTON010000955         1094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_109277_length_1094_cov_4.039461, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000955 DAOTON010000000
VERSION     DAOTON010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109277_length_1094_cov_4.039461"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="P5292_14170"
     CDS             complement(<1..677)
                     /locus_tag="P5292_14170"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRS40322.1"
                     /translation="MDYNEIAALIRLLDDSDSEIFTQIEQRLLHYGKPVIPFLEKAWG
                     GSLDALMQQRIENIIHRIQFEDLQRELRVWVETGAQDLIKATVMVARYQYPELKEADI
                     RSQLEKIRKDVWLEVNDGLTALEHIRVLNRVFFEIHGFSGNTQHYHAPQNSFINIALE
                     SRKGNPLMLSILYQEVAQGAGIPVYGVNLPEHFILCYKEDADPGSGSAGQVLFYVNPF
                     SKGDVFHR"
     gene            904..>1094
                     /locus_tag="P5292_14175"
     CDS             904..>1094
                     /locus_tag="P5292_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40323.1"
                     /translation="MKQICSIALVLISLVLSNSLLAQGSLMWSSGYAGSGDNSDCFNK
                     IISDDNSGSLIAVGYEMREG"
BASE COUNT          273 a          275 c          279 g          267 t
ORIGIN      
        1 cgatggaaaa catccccttt gctgaacggg ttgacataga agagtacctg acctgctgaa
       61 cctgatccgg gatcggcgtc ttctttgtaa cagaggatga agtgctcagg cagattgaca
      121 ccgtaaaccg gaattccggc accctgagcg acttcctgat acaggatgct caacataagc
      181 ggatttcctt tacgggattc cagtgctatg ttgataaagg aattttgagg agcgtggtag
      241 tgctgtgtgt tgccggagaa cccgtggatc tcgaaaaaca cccgattcag tacccggata
      301 tgctccagag ccgtgagtcc gtcgttcact tccagccaga catccttacg gatcttttcc
      361 agttgactgc gaatatccgc ctctttgagc tccgggtatt ggtatcgggc aaccatgacc
      421 gttgccttga tgaggtcctg agcgccggtt tcgacccata cacgcaactc acgctgtagg
      481 tcttcgaact ggatacggtg gatgatattt tcaatccgtt gctgcatcaa cgcgtccagc
      541 gagccacccc aggctttttc aagaaagggg atgacgggct tgccataatg caacaggcgt
      601 tgttcgatct gggtgaagat ctccgaatca gaatcatcca gaagtcggat aagggcagcg
      661 atttcgttat agtccatacg tcggttttcc tgaccagttt aaccgaagcg aggccccgca
      721 ggatacaccg gaaacaaaac cgttttcaac ataaacatgt tggaaccccg gaaagccttg
      781 cctggagcgg gaatccggga ttaaaatgcg gctaaaccgg ttgaaaatcc gacattcccg
      841 tgatcgccgg cgcggcggat tccactattt ttgcagttca ttcgaataag aaaacccttc
      901 tacatgaaac aaatttgttc cattgccctg gtattgatct ccttagtact gagcaacagc
      961 ctgctggcac aaggcagcct gatgtggtca tccggttatg ccggctccgg ggacaattca
     1021 gactgcttca acaagatcat ttcagacgat aattccggat cgttgatcgc agttggatac
     1081 gaaatgcgcg aagg
//
LOCUS       DAOTON010000956         1091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_109759_length_1091_cov_2.650579, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000956 DAOTON010000000
VERSION     DAOTON010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109759_length_1091_cov_2.650579"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="P5292_14180"
     CDS             complement(<1..1017)
                     /locus_tag="P5292_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40324.1"
                     /translation="MRNLYHLLVLAFLLPAIAEAQITIQSADLPSAGDTFRVSTAAPT
                     AGIDLTTTGANAVWDFSQLVSNGQTIDTFLSVNATASVFSVVFSDLPFNPNRANQATR
                     GQAFNLGTVNVSDVFNFFYNSSTQYKQPGFGATVNGAQLPIIYSSHDVHYEFPLSFGN
                     TAASNSGYELDLTSTLGFFFQVDRNRQTVVDGWGSVTTPHGTYNALRVVSTIVEQDSV
                     YIDSLGFGLNLPAITTVEYKWLANGEGIPVLQINTTATGTVTQIRYKDAPLTTGVQSL
                     NIISDLTAYPNPASNILTVRWNAANAAPVSLELRDLQGRIVFSNQLHGVAGTNIRVVD
                     LASLH"
BASE COUNT          248 a          271 c          319 g          253 t
ORIGIN      
        1 gtgaagtgag gccaggtcca ccacccggat attggtacct gcaacgccat gcaactggtt
       61 agaaaaaacg atccgaccct gcaggtccct cagttcaagg gataccggtg ctgcattggc
      121 cgcgttccag cgtacggtca ggatgttgct ggccggattc ggataggccg tcagatcact
      181 tattatattc agtgactgaa ctccggttgt aagcggagca tctttgtaac ggatttgtgt
      241 aaccgtgccc gttgccgtgg tattgatctg caatacggga atcccctctc cgtttgccag
      301 ccatttgtat tcgacggtcg tgatcgccgg gaggttcaaa ccaaatccaa gcgagtcgat
      361 gtagacactg tcctgttcga caattgtact gacaacccgc aaggcattat aagttccgtg
      421 cggagtcgtg actgaccccc atccatccac gacagtctgc cggttgcgat ccacctggaa
      481 gaagaatccg agggtgctcg tcaggtccag ttcgtagccg gagttcgaag cagcggtatt
      541 tccgaacgag agcgggaatt catagtggac atcgtgcgac gaatagatga tcggcagttg
      601 ggcgccgttg acagtcgcgc cgaagccggg ttgcttatac tgcgtgctgc tgttgtagaa
      661 gaaattgaag acatcgctga cattcaccgt tccaaggtta aacgcctggc cccgggttgc
      721 ctgattagcg cggttcggat tgaacggaag gtcgctgaac acgacggaaa acaccgaagc
      781 ggtcgcattg acacttaaga acgtatcgat cgtttgcccg ttggacacca actgggaaaa
      841 atcccatacg gcattggcgc cggtcgtggt cagatcgatt cccgcagttg gtgcagcggt
      901 actgacccgg aaggtgtctc cggcggaagg aagatcggcg gattggatgg tgatttgtgc
      961 ttctgcgatc gcaggcaaca ggaaagccag cacaagcaag tggtacaggt tacgcatttg
     1021 ggtgatagtt tccggtaaag ataccgttct cgtttggttg tcgtcggagt gcgactgaaa
     1081 ttcacccttg c
//
LOCUS       DAOTON010000957         1089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_110073_length_1089_cov_2.555126, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000957 DAOTON010000000
VERSION     DAOTON010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110073_length_1089_cov_2.555126"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="P5292_14185"
     CDS             complement(<1..1062)
                     /locus_tag="P5292_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HRS40325.1"
                     /translation="MDFFEGPVADPSDGVDNDRDSLIDAADTNQIGGEQIIMSRFVYY
                     NNDGSNIGNPNNAQQYYNYLRGIWKDGSPLVYGGNGYQTSGADSCDFMFPGDTDPNGW
                     GTNGTPLSQLFPWSETEPTPGGTPNQPDDRRFLQSAGSFTLQPGAVNYITTGAVWARA
                     SSGGPLASVKLLRITDNTAQDLFNNCFKVLDGPDAPNLVIRELDREVIFSIVNPTSSN
                     NYRELYAEVDKKAIDNGFTGSQFTFEGYKIFQLKDAAVSVTDLTDADKARLVFQCDIK
                     NGVSRIVNQYLQAETTTFLPVVEVEGADEGLKHSFRLTTDAFATGDPNMVNHKTYYFM
                     AIAYGYNKDQEVFDPYESAL"
BASE COUNT          242 a          277 c          316 g          254 t
ORIGIN      
        1 gagtgcgctt tcgtaaggat cgaatacttc ctggtccttg ttatagccgt acgcaatcgc
       61 catgaagtag tacgtcttat gattgaccat gttcgggtca ccggtggcga atgcatccgt
      121 ggtaagacgg aacgaatgct tcaagccctc atccgctcct tccacctcaa cgaccggaag
      181 gaaggtggtc gtttcagctt gcaggtattg gttgacgatc cggcttacac cattcttgat
      241 gtcgcactgg aatacgagtc tggctttgtc ggcatccgtg aggtcggtca ccgatacggc
      301 ggcatccttc agttggaaga tcttatagcc ttcgaaggtg aactgggagc ctgtgaaacc
      361 attatcaatg gctttcttgt ccacttccgc atacagttca cggtagttgt tggaagaagt
      421 tgggttgacg atcgagaaga tcacttcacg atccaattcc cggatcacca ggttcggcgc
      481 atccggacca tccaacactt taaagcagtt gttgaacagg tcctgagcgg tgttgtcggt
      541 gatgcgcagc agtttcacgg atgccagggg gccaccggaa gaagcgcggg cccaaacagc
      601 accggtcgtg atgtaattga cggcaccggg ttgcagggtg aatgaaccgg cagactggag
      661 gaaacgacgg tcatccggct ggttcggtgt accgcccgga gtaggttcgg tttccgacca
      721 ggggaacaac tgactcagcg gagtgccgtt ggtaccccag ccattcggat cggtatcgcc
      781 cgggaacatg aaatcacaag agtcagcacc ggaagtctga taaccgtttc cgccgtaaac
      841 cagcggagag ccgtccttcc agattccgcg caagtagttg tagtactgtt gcgcgttgtt
      901 cgggttaccg atgttggatc cgtcgttgtt ataataaacg aaacgcgaca tgatgatctg
      961 ttcaccgccg atctggttgg tatcggcagc atcgatcagg gagtcacggt cgttgtcaac
     1021 accgtcgcta gggtcggcca ccggaccttc gaagaagtcc atgccgatcg cgggaggatt
     1081 ggcaccata
//
LOCUS       DAOTON010000958         1088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_110191_length_1088_cov_3.680542, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000958 DAOTON010000000
VERSION     DAOTON010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110191_length_1088_cov_3.680542"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="P5292_14190"
     CDS             complement(<1..430)
                     /locus_tag="P5292_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HRS40326.1"
                     /translation="MRATNLLLLFLLTFFCAGVSRAQQIPLLERKISIRAVNRPVDEF
                     LQETGVVAGCTFSYAATVTRGMQPVTLDHRDRPLRELLDRIFSGKVNYKERGTHVILL
                     RVQPVDQEPEPTLFSVAGYVTDASTGERIASVTVYDKYSRA"
     gene            complement(417..>1088)
                     /locus_tag="P5292_14195"
     CDS             complement(417..>1088)
                     /locus_tag="P5292_14195"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRS40327.1"
                     /translation="FLVAPVAGPETILAAADSSITRALPDGSTISLNRGSTLTYASAS
                     YAKQRRVKLKGEAFFEVRHDAEHPFIVEAGGLEIRDVGTAFNIQAREDNRLVVITVTE
                     GEVSVSIPKHGDEALRPGQELVYDTEAGSVERRENNDPNIAAYKDKIFIFEDASLGVV
                     IHTLQDVYSVRMYLENERLGDCRITATFNDEPIEEITQIIAETLGLEASRTDSGYVFN
                     GNACY"
BASE COUNT          220 a          291 c          333 g          244 t
ORIGIN      
        1 atgcgcggct gtacttgtcg tacaccgtca cagatgcaat gcgttctccc gttgacgcgt
       61 ccgtcacgta accggcgacg gagaagaggg taggctccgg ttcctgatca accggctgca
      121 cccgtaggag tatgacatgt gttccgcgct ccttgtagtt gacctttccg gaaaatattc
      181 ggtcgagcaa ttcccgcagc ggacggtcgc ggtgatccag ggtcaccggt tgcatgccgc
      241 gggtcaccgt tgcggcgtaa ctgaaggtgc agcccgcgac gacgccggtt tcctgcagga
      301 attcatcaac ggggcgattg actgcccgaa tgctgatctt gcgttccagt aagggtatct
      361 gctgtgcacg cgaaacgcct gcacaaaaga aagtcaacag aaaaaggagg aggaggttag
      421 tagcacgcat tgccgttgaa aacatacccg ctgtcggtgc gactggcttc caggccaagc
      481 gtttcagcga tgatctgagt gatttcttcg atcggttcgt cattgaaagt tgccgtgatg
      541 cggcagtcac cgagtcgttc attttccagg tacatccgta cggaataaac atcctggagc
      601 gtatggatca ccacgccgag ggaggcgtct tcgaagatga agattttgtc cttgtaggcg
      661 gcgatgttcg ggtcgttatt ctcccggcgt tcaacgctgc ccgcttcggt atcgtagacc
      721 agttcctggc caggccgcag ggcttcatcg ccatgcttcg gaatgctgac gctgacctcg
      781 ccttcggtca cggtgatcac gaccaaccga ttgtcttcac gggcctggat gttgaaggcc
      841 gtaccaacgt cgcggatttc aagcccgcct gcttcgacga tgaatggatg ctccgcatcg
      901 tggcgcactt cgaagaatgc ttcgcctttc agtttgaccc ggcgttgttt ggcataggaa
      961 gcggaagcat acgtaagcgt actgccccgg ttgagggaga tggtggaccc gtcgggtaac
     1021 gcacgggtga tggaagagtc cgcagcggcg aggatggttt caggtccggc taccggcgcg
     1081 acgaggaa
//
LOCUS       DAOTON010000959         1086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_110656_length_1086_cov_1.347236, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000959 DAOTON010000000
VERSION     DAOTON010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110656_length_1086_cov_1.347236"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="P5292_14200"
     CDS             complement(<1..330)
                     /locus_tag="P5292_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40328.1"
                     /translation="MIQKFLLTVFLFVFGAINAQAQGPNITSWLQNTTGITGRHYVAG
                     NSTPIVDAALANIQTVQYSTNWVYVSTNGIPAYITGPFLDGNPSIAQSQNAIFRMPLN
                     PVQNTGTP"
     gene            complement(462..>1086)
                     /locus_tag="P5292_14205"
     CDS             complement(462..>1086)
                     /locus_tag="P5292_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRS40329.1"
                     /translation="NTLDEVLKRLRRSPLYPELFRAVFGDTVISGQRFLKAISAFMLT
                     LVSGDARYDRMKRGELTFTEQEANGYRLFQEHCNACHREPLFTNYAYANNGLLPDTAV
                     PDAGRYRITRVASDSLQFKVPSLRNLEYSYPYMHDGRFKTLSEVIRHYTNGIQIEPTL
                     SSELQKPIVLRPEEKVDLQAFLLTLSDSAFVFDTSHAFPRQLFFGKR"
BASE COUNT          252 a          274 c          327 g          233 t
ORIGIN      
        1 cggtgtgccg gtgttctgca ccggattcaa tggcatgcgg aagatcgcgt tctggctctg
       61 cgcgatcgaa ggattcccgt cgaggaaggg tccggtgata taagccggta tgccgttcgt
      121 actcacgtac acccagttcg tcgaatactg cacggtctgg atgttggcca gggcagcgtc
      181 aacgatcggc gtggaattgc cggcaacata atgccggccg gtaataccgg tcgtgttctg
      241 taaccaggag gtgatgttgg ggccctgcgc ctgggcgttg atggctccga atacgaaaag
      301 gaaaaccgtc aggaggaatt tctggatcat gggtgggtgc gggatgagga ccttggatga
      361 ctgaaacctc cgaaggttta tcgaatatcc ggaattccgg gagaattatt tcgctattca
      421 ccgataaaaa caccggcctg gccgacaccg ttgtcgtacg ctcagcgttt gccgaaaaac
      481 aactgccggg ggaaagcatg gctggtgtca aaaacgaaag cgctgtcgga aagcgtcaac
      541 agaaaagctt gcagatcgac tttctcctcc ggacgcagca cgatcggctt ttgtagttcc
      601 gacgatagtg ttggttcgat ttgaatgccg ttggtgtaat gccggatcac ctcagaaagt
      661 gtcttgaagc gaccatcgtg catataagga taggagtact ccaaattccg cagactggga
      721 actttgaact gcagactgtc ggatgcaacg cgcgtgatcc ggtaacggcc cgcatcgggc
      781 acggcggtgt caggaagcaa tccgttgtta gcataggcat agttggtaaa caatggttcg
      841 cggtgacagg cgttacaatg ctcctggaag agtcgataac cgttcgcctc ctgctccgtg
      901 aacgtcaact ccccgcgctt catccggtcg tagcgcgcgt cgccgctgac cagcgtaagc
      961 atgaaggcgg agatcgcctt gaggaaccgc tgaccgctga tcaccgtatc gccaaaaaca
     1021 gcgcggaaca attccggata caaaggcgag cgccgcaggc gtttcagcac ctcatcgagg
     1081 gtgttg
//
LOCUS       DAOTON010000960         1085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_110689_length_1085_cov_3.633010, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000960 DAOTON010000000
VERSION     DAOTON010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110689_length_1085_cov_3.633010"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1036)
                     /locus_tag="P5292_14210"
     CDS             complement(119..1036)
                     /locus_tag="P5292_14210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRS40330.1"
                     /translation="MNNKYTIFFALVLIFLASCQQNGTEKKTASGFTYRVYKNIDGPK
                     PKVGDYVTIHLVNRTADDSILFDSRKAGQPMRFRLERIPFEGSFEEGLTYISPGDSAT
                     FYVPADSLYDYLYKRKGIQVEQEKTSFRKGSMIRFDISLLRVEDYVTAEQEMLQRASE
                     MEKKERESLAGYVQAQRLDSLRQPEGYWLQQVEAGKGEKLDSGNVVVVEYTGYKLDGT
                     VVDDSRNSGKAYRFLPGARQVVPGWELAVKGKRVGDRLKIVLPSSLAYGAEGFSDPRT
                     GTYLIEPYTPLVFDIRILAQEPVEVSAGK"
BASE COUNT          244 a          299 c          264 g          278 t
ORIGIN      
        1 aaaccgaaca agagctcgtt cagacaagca gccttgccga ttggaaaaag aagaaaccgg
       61 gtttacataa acgtccctgt gtcttcatcc ttggtgcgta aatccaaaca gtggacggtt
      121 actttcctgc acttacttct accggctctt gtgcaaggat cctgatgtcg aacaccaggg
      181 gcgtgtacgg ttcaatcagg taggtacccg ttctggggtc gctgaatcct tccgctccgt
      241 aagccaaaga agaaggcaat acaattttca atcggtcgcc gacgcgtttc cccttcacgg
      301 ccagttccca accgggtact acttgtcggg cgcccggcag gaagcgatag gcttttcctg
      361 aattacggct gtcgtctacg accgtaccat cgagtttgta gccggtgtat tcgacgacga
      421 cgacattacc cgaatccagt ttctcccctt ttcctgcctc cacctgttgc agccagtagc
      481 cttccggctg ccggagtgaa tcgaggcgct gagcctgtac ataaccggcc aaggactccc
      541 gttctttctt ttccatttcg gaagcacgtt gcaacatttc ctgctccgcc gtcacataat
      601 cctcgacgcg cagcaagctg atatcaaacc ggatcatgga tcctttccgg aagctcgtct
      661 tctcctgctc tacctggata ccctttcgtt tatacaggta atcgtacaag gaatcagccg
      721 gcacataaaa agtagcgctg tcgccgggcg agatgtaggt cagaccttct tcgaatgatc
      781 cttcgaacgg aattcgctcc aaccggaacc gcatgggctg tccggctttt cttgaatcaa
      841 agaggatgct atcgtccgcc gtacgattga cgaggtgaat ggtcacatag tcaccgactt
      901 tcggtttggg gccgtctatg ttcttgtaaa cccgataggt gaaaccgctg gcggtctttt
      961 tttccgtacc attctgctgg caacttgcca gaaagatcaa cacgagggcg aagaatatcg
     1021 tgtacttatt gttcatgggt tgactttctt taactcgaga tccaggtaaa ccggtgtggc
     1081 accgg
//
LOCUS       DAOTON010000961         1084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_110848_length_1084_cov_3.439261, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000961 DAOTON010000000
VERSION     DAOTON010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110848_length_1084_cov_3.439261"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="P5292_14215"
     CDS             complement(<1..271)
                     /locus_tag="P5292_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRS40331.1"
                     /translation="MRSGIKKFGFLYSIALLALAGCGSGNKGELTGVLHREKWYQADP
                     YGMVYIPAGSYNMGPSDQDVNYSLTAQSKTVTIPAFYMDHTEITNN"
     gene            complement(426..>1084)
                     /locus_tag="P5292_14220"
     CDS             complement(426..>1084)
                     /locus_tag="P5292_14220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRS40332.1"
                     /translation="ADQLGAEKNLNIRGAYAYQAELGMGRLGVGVDVGYHQKSLDGSK
                     FVFNDAGDGNIPTGNVSGGSLDLGLGVYYATDRLYVGLSSTHLQEGDIKYDNVRTSLA
                     RHYWLMAGYSLDLTPSLTLKPAMLVKSDAVSTQLDVNANLMINNRFWVGAGYRLQDAV
                     IVTAGLEIMPNLKLGYSYDYTTSDIRTYSSGTHEVMLGYCFRPNKPIKRMFHRNVRML
                     "
BASE COUNT          228 a          300 c          290 g          266 t
ORIGIN      
        1 cgttgttcgt gatctcggtg tggtccatgt agaacgcagg gatcgttacg gtcttgctct
       61 gggccgtgag ggaataattc acgtcctggt cgctcggacc catattgtac gatcctgcag
      121 gaatgtatac catgccgtaa ggatcggcct gataccattt ttcccgatgg agcaccccgg
      181 tcaattctcc cttgtttccg ctgccgcagc cggcaagcgc caggagagcg atggaataga
      241 gaaacccgaa cttctttatc cctgatctca tccgattcat ggtctttgag gtgaaacgcg
      301 attagcgtgc taacgcagtt tttacgaact tattgaatat caattaacct tcgttctata
      361 acgagggcct cccccacggg tttcatcaaa tctaggctac gggacagtcc ccgccgcttc
      421 aattttcaga gcattcttac attccggtgg aacatgcgtt tgataggctt gttcggccgg
      481 aaacagtatc caagcatcac ttcatgggtt ccgcttgagt aggtccgtat gtcggaggtc
      541 gtatagtcgt aagaatatcc cagtttcagg ttcggcatga tctccaggcc ggccgtcacg
      601 atgacggcat cctgcaggcg gtaacccgcg cctacccaga accggttgtt gatcatcagg
      661 ttggcgttca catccagttg ggtggaaacg gcatccgatt tcacgagcat tgccggcttc
      721 agcgtcagtg atggcgtaag atccaggctg tagccggcca tgagccagta atgacgggcc
      781 aggctggtcc gtacgttatc gtatttgatg tcgccttcct gcaaatgcgt gctcgacaaa
      841 ccgacataca gtcgatcggt cgcatagtac acgcctaagc ccaggtcgag cgaaccgccg
      901 ctcacgttcc cggtggggat gttgccgtca ccggcatcgt tgaagacgaa cttggaaccg
      961 tcgagcgact tctgatggta gccaacgtcg acacccacgc ccagccgacc catgccgagt
     1021 tcagcctggt atgcgtaagc accgcgaatg ttcagattct tttccgctcc gagttggtcg
     1081 gccg
//
LOCUS       DAOTON010000962         1084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_110862_length_1084_cov_3.014577, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000962 DAOTON010000000
VERSION     DAOTON010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110862_length_1084_cov_3.014577"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..>1084)
                     /locus_tag="P5292_14225"
     CDS             complement(232..>1084)
                     /locus_tag="P5292_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40333.1"
                     /translation="PQKDMILRIVASNSDPEAREMEIKKMGKAYTEIAEGVLPKLRRS
                     AISINGEKVGRSDEQIAALAKSNPDSLSVEEILYAGTLTQDLNEKLSIYRTAQRLYPQ
                     EWRAANNTGMILFQQGDVDGAMAEFNKADQLNANNGIVKNNIGACYSRKGDRANAAAA
                     YAAAGNAGPEVKENMGILDIRNGNYSSAVSNYSGVSTFNAALARLLSGDKEGAMMTLE
                     ASPDKDSAIGFYLKAIIAARNGNAAGVTDNLRNACAKDASLKAAAAEDREFIKWFNDA
                     GFKASIQ"
BASE COUNT          213 a          283 c          325 g          263 t
ORIGIN      
        1 cgtcccgacg cgcgaccagc agcaacggct ggcacgtgaa cgagccaatg tattcgttgg
       61 tgagagcccg gctccggaag tgatccggga gatgagaatc ctttttgatc tgccgacaaa
      121 accgggagaa agcatctgac caactctacc gcattccgcg atgcgccaaa agaaaaaggg
      181 tgtccgacca agcagtcgga cacccttttt taatggtgtt ctatgagtgg attactggat
      241 cgaagccttg aagccagcgt cgttgaacca cttgatgaat tcgcggtctt cagcggcagc
      301 agccttgagg gaagcgtcct ttgcacaagc gttgcggagg ttgtccgtta caccggcagc
      361 gtttccgtta cgggcagcga taatggcttt gaggtagaaa ccgatcgcac tgtctttgtc
      421 gggcgaagcc tcgagcgtca tcatagcgcc ttctttgtca cccgagagca aacgagcaag
      481 cgcggcgttg aaggtggata ctccggagta gttggagaca gccgagctgt aattaccgtt
      541 gcggatatcg aggataccca tgttctcttt tacttccgga ccggcgtttc ctgctgcggc
      601 gtaggctgct gcggcattgg cgcgatcacc tttgcggctg tagcaggcac cgatgttgtt
      661 cttcacgata ccgttattgg cattgagctg gtcagccttg ttgaactcgg ccatggcgcc
      721 gtcgacatca ccctgctgga agaggatcat accggtgttg ttggctgcgc gccactcttg
      781 cgggtacaag cgctgtgccg tgcggtagat cgagagtttt tcgttcaggt cctgggtgag
      841 ggtaccggca tagaggatct cctcaacgct cagggaatcc ggattcgact tcgccagggc
      901 tgcgatctgt tcgtcggagc gacccacttt ctcgccgttg attgaaatcg cggagcgacg
      961 cagtttcggc agtacgcctt cagcgatctc ggtgtaggct ttgcccattt tcttgatttc
     1021 catttcgcgg gcttccggat cagagttgga agctacgatg cggaggatca tgtccttctg
     1081 gggc
//
LOCUS       DAOTON010000963         1084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_110873_length_1084_cov_2.839650, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000963 DAOTON010000000
VERSION     DAOTON010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110873_length_1084_cov_2.839650"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1084
                     /locus_tag="P5292_14230"
     CDS             <1..>1084
                     /locus_tag="P5292_14230"
                     /inference="COORDINATES: protein motif:HMM:NF040284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40334.1"
                     /translation="GVSGATPCASSCGTSINQTLTNGGTSNGTATYTVTPTANGCPGA
                     PYDVVVTVKPIPAAIIAPSTESFCGSGTTGFALSSPVSGATFSWTASQSNATGASASS
                     GATIAQLLTLNSGSPTGSVTYTVTPTANGCNGSTATATVSLLAAPSAVSASPSSTTIC
                     PGASVALAASGGLGSGSFVSGTGSSTSTALSNTSTLGPNPLQNYYGGSKQQMLWRASE
                     LTALGLTAGMQITSISINLGAANTSLALNSFRVKVQHTALTALTTSPVSTGWTTVYGP
                     TNYTPNTGQNVFTFSSPFTWNGTSNLLVEMNFSNNSSPTTGTNAATFNTGIGFTATTL
                     YRVDNQTPATLDGYTGTMTYSYTSRNN"
BASE COUNT          237 a          334 c          259 g          254 t
ORIGIN      
        1 gggcgtatcg ggtgcaactc catgtgcttc ttcctgcggt acgagtatta accagacact
       61 gacaaatggc ggaacctcaa atggcaccgc aacctatacc gttactccga ctgccaatgg
      121 ctgccctgga gcgccttacg atgtagttgt gactgtgaaa ccgatacctg ctgccatcat
      181 tgccccgtca actgaatcct tctgtggctc cggaaccacc ggctttgcat tgtcgagccc
      241 cgtttccggt gcaacctttt cctggacggc cagccagtcc aatgcaaccg gtgcatcggc
      301 ttccagcgga gctacgatag cgcaattgct tacccttaat tccggaagcc ccaccggatc
      361 tgttacctac accgtaactc ctacggccaa cggctgtaac ggttcgacag ctactgccac
      421 cgtgagctta ctggctgcgc cttcggctgt ttcagcttca ccttcatcta cgacaatctg
      481 tcctggtgcg agcgttgcac ttgccgcgag tggaggactg ggatcaggaa gtttcgtttc
      541 aggaacgggt tccagcacca gcacggctct atctaacacc agcacgcttg gaccgaaccc
      601 gctgcagaat tactacggcg gttcgaagca acaaatgctc tggcgtgcga gtgaactgac
      661 ggcccttggt ttgacggccg gaatgcagat cacttccatc tccattaacc tgggcgctgc
      721 caacacatcg cttgccctga acagcttccg cgttaaggtg caacacacgg cgcttactgc
      781 cctgacgact tccccggtat cgaccggctg gacaacggtc tacggtccta cgaattatac
      841 gccgaacacc ggccagaacg tattcacgtt ttcgtctccc tttacctgga acggtaccag
      901 taacctgttg gtggaaatga acttttcgaa caatagttcg cccactaccg gtaccaatgc
      961 tgccaccttc aatacgggta taggttttac ggcaactacc ttgtatcgtg tcgataacca
     1021 gacccctgcc acattggacg ggtataccgg cacgatgact tattcctata cctcgcgtaa
     1081 caat
//
LOCUS       DAOTON010000964         1083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_111032_length_1083_cov_3.158560, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000964 DAOTON010000000
VERSION     DAOTON010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111032_length_1083_cov_3.158560"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..810
                     /locus_tag="P5292_14235"
     CDS             40..810
                     /locus_tag="P5292_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRS40335.1"
                     /translation="MTGLQAQPAVIWPVRTEPFVLGITDIIRSTVMNEERTLNIYLPP
                     GYNEQDSVRYPVIYLLDGSVDEDFIHMAGLVYFLGFPWLNLCPPSIVVGIANVDRKRD
                     FTFPTTVEKDKKDFPTTGGSAAFIRYLGEEVIPYIRSRYKTNNTSTLIGQSFGGLLAT
                     EVLFTHPDWFSKYLIVSPSLWWDNQSLLNRTVTLATQPDVRIAVGKEGRVMERDAKAL
                     YRKLRPFYADKRRLRFHFFKKENHGSILLHAAYEGLQK"
     gene            862..>1083
                     /locus_tag="P5292_14240"
     CDS             862..>1083
                     /locus_tag="P5292_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40336.1"
                     /translation="MHQIRVDAQAGAAWKRLSKAEKRWVIGHLFVAKRAYRISLEARD
                     LATKLEQDARLDHDKDGGEVDAFRHAYWMA"
BASE COUNT          237 a          286 c          318 g          242 t
ORIGIN      
        1 cgcggaacgg ccgtactcgc ttgcctgttg tttctgacgt tgaccggtct ccaagcgcaa
       61 cctgctgtaa tctggccggt gagaaccgag ccgttcgtgc tggggatcac ggatatcatt
      121 cgctcgactg tgatgaacga ggagcgaacc ctgaacatct acctgccgcc cggttataac
      181 gaacaagata gtgtgcgtta cccggtcatt tatctgctcg acggctcggt ggatgaggat
      241 ttcattcaca tggccgggct ggtgtatttc ctcggatttc cctggctcaa cctgtgtccg
      301 ccatcgatcg ttgtggggat cgccaacgtc gaccgcaagc gcgacttcac cttcccgacg
      361 accgtcgaga aagacaagaa ggatttcccg accaccggcg gctcggcagc ctttatccga
      421 tatcttggcg aagaggtgat accctacata cggtcccgtt ataagacgaa taatacgagt
      481 acgctgattg gtcaatcatt cggcggacta ttggctaccg aagtattgtt tacccatccc
      541 gactggttct cgaagtacct gatcgtgagc ccaagtttgt ggtgggacaa ccagagcctt
      601 ctgaaccgga cggtcaccct ggcgacgcag cccgatgtca ggatcgcggt cgggaaggag
      661 ggacgtgtga tggaacgcga cgcgaaagca ctctaccgta agctgcgacc cttctacgca
      721 gacaagcgac gattgcgttt tcactttttc aagaaggaga accatggttc catactgctg
      781 cacgccgcct atgagggatt gcagaaataa acttgccggg atatgaagcg tgttgggggg
      841 atgctcctac tctgttgcct gatgcatcaa attcgggtcg acgcacaggc cggtgctgcc
      901 tggaagcgcc tcagcaaagc ggaaaagcgg tgggtcatcg ggcacttgtt tgtcgcgaaa
      961 cgcgcttacc gcatttcgct ggaggcgcgt gaccttgcaa cgaaattgga acaggacgcg
     1021 cgactggatc atgataagga tggaggggaa gtggacgcgt ttcggcatgc ttactggatg
     1081 gcc
//
LOCUS       DAOTON010000965         1082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_111180_length_1082_cov_2.949367, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000965 DAOTON010000000
VERSION     DAOTON010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111180_length_1082_cov_2.949367"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1082
                     /locus_tag="P5292_14245"
     CDS             <1..>1082
                     /locus_tag="P5292_14245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRS40337.1"
                     /translation="TEADLFANYIRRWNMLPQVCEDSAKALDLVRRESGLNLVIIDST
                     LVSGNAMELAESIRHLRPKDELPIVLFNADRTDNILFDYTSDVVSAVIPKNVDRSKVL
                     DILIGVFSVEDHQRSQHEEGLSRVGASLAEEFPVRILIAEDNQINQKLAQNIFEGLGY
                     RPVIVSNGQEVIDQLRRQVFDIIFMDVQMPELDGLEATRFIMNKMNLQHLPVIVAMTA
                     FALEGDKEKCLEAGMDDYISKPFMIEEIVERIRKWSKGREQAAHEEPSVPVVVVSSAT
                     SALIDQTVLDRLREMTAGSDPGFLVQVIGMFIEQARDIYHDLERAGASRDHLAISRLS
                     HKLKGSALNIGAKRIAETCRELELRS"
BASE COUNT          268 a          264 c          313 g          237 t
ORIGIN      
        1 acggaagctg atctctttgc caattacatc cggcgatgga atatgcttcc tcaggtttgt
       61 gaggactccg ccaaggcgct cgacctggtc cgtcgtgaaa gcggactcaa cctggtgatc
      121 attgacagta cgcttgtgag cggaaatgcg atggaactgg cggaaagcat ccgtcacctt
      181 cgtccgaagg atgaacttcc gatcgtcctg ttcaacgcgg accgtacgga caacatcctc
      241 ttcgattata cttcggatgt ggtttcggcc gttatcccta agaatgtcga ccgcagtaag
      301 gtgctggata tcctgatcgg agtattctct gtagaggacc atcagcgcag ccagcacgag
      361 gagggattgt cgcgagtagg agcaagtctc gcagaggaat ttccggtgcg catcctgatc
      421 gcggaggata accagatcaa ccagaaactg gcgcagaaca tctttgaagg cctgggctat
      481 cgtccggtga tcgtttcgaa tggacaggaa gtgatcgatc agcttcgccg acaggtcttc
      541 gacatcattt tcatggatgt acagatgcct gagttggatg gattggaggc gacaagattc
      601 atcatgaaca agatgaatct gcaacacctt ccggtcattg ttgccatgac cgcattcgcg
      661 ctggaaggcg ataaggaaaa atgcctggaa gccggaatgg acgattatat cagcaagccc
      721 ttcatgatcg aagagatcgt agagcggatc cgtaagtgga gcaagggccg tgaacaggcg
      781 gcgcatgaag aaccctcagt tccggtagtg gtggtttcct cggctaccag tgcgctgatc
      841 gaccagacgg tactcgacag gctccgcgaa atgacggctg gcagtgatcc cggattcctc
      901 gtacaggtta tcgggatgtt catcgagcag gctcgggata tttaccatga tctggagcgg
      961 gcaggagcaa gcagggacca tcttgcgatt tcacgactat cgcataaact caaaggttcg
     1021 gcactgaaca tcggagccaa gcgaattgcg gaaacctgtc gggaactcga gctcaggtcc
     1081 aa
//
LOCUS       DAOTON010000966         1077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_112084_length_1077_cov_2.727984, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000966 DAOTON010000000
VERSION     DAOTON010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112084_length_1077_cov_2.727984"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="P5292_14250"
     CDS             complement(<1..579)
                     /locus_tag="P5292_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HRS40338.1"
                     /translation="MATTPTLSGLELTKWRSKFITEYIRDTGFDTYMGDADTDIIHVV
                     NDLQTDGYTIRIPLMGKLGGSGVTGNTSLTGNEQQLDQYYQDISWEFRRQAVLATKKD
                     REKSAVDFMAQARPRLKEWSTENVKYRLIECFHKMSDGTPFSAADATTRNAFSAKNKD
                     RILYGSTQANYSGTHATGLTAIDNTDDKLSTKV"
     gene            complement(636..>1077)
                     /locus_tag="P5292_14255"
     CDS             complement(636..>1077)
                     /locus_tag="P5292_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40339.1"
                     /translation="QQEQFQQALLNQRLNTSEMFARNKHGDQIVDAAFQAAQQAGVLK
                     SFVGSRDCYGELVKWHRHQQVLARVGEDPDAYEKTLEDKIRAKVLEELKTGNRAVMPG
                     QTPPPVQQFPGSLADATGTGSNAAVPVSDEAIMGNVFGSNRKRK"
BASE COUNT          175 a          295 c          338 g          269 t
ORIGIN      
        1 taccttggtc gacagtttgt cgtccgtgtt gtcgattgcg gtcaggccgg tcgcatgcgt
       61 gcctgagtag ttcgcctgcg tgctgccgta gaggatgcga tccttgttct tggcagagaa
      121 cgcatttcgc gtggtcgcat cggctgccga gaacggcgtg ccgtccgaca ttttatggaa
      181 gcactcgatc agccggtatt tgacgttctc ggtcgaccat tctttcagac gcgggcgcgc
      241 ctgggccatg aaatcgacgg cggacttttc tcggtccttc ttcgtcgcga gcacggcctg
      301 gcgccgaaac tcccaggaaa tgtcctggta atactgatca agctgctgtt cgttgccggt
      361 caacgaggtg ttgccggtga cgccggagcc gccgagcttg cccatcagag ggatgcggat
      421 cgtgtatccg tccgtctgca ggtcgttcac gacgtggatg atgtcggtgt cggcatcgcc
      481 catgtaggtg tcaaagccgg tgtcacggat gtactccgtg atgaacttcg agcgccattt
      541 ggtaagctcg agcccggaaa gggtcggagt ggttgccatg ttggatgatc tttcgtgatc
      601 aggggttggg gatcacgaag cgcgtctgat gcggcttact ttcgctttcg gtttgaaccg
      661 aaaacgttgc ccatgatggc ttcgtcgcta acgggaacag cagcgttcga tcccgttccg
      721 gtcgcgtctg cgagggagcc tgggaactgc tgtactgggg gcggcgtctg tcccggcatc
      781 actgcccggt ttccggtctt caattcttcc agcaccttgg cgcggatctt gtcctcaagc
      841 gtcttctcat aggcgtccgg gtcttcaccc actcgggcta agacctgctg atggcgatgc
      901 catttcacca gttcgccgta gcaatctctg ctgccgacga aggatttgag aacaccggcc
      961 tgctgcgctg cctgaaacgc ggcgtcgacg atctgatcgc cgtgcttgtt ccgggcaaac
     1021 atctcggagg tgttcaagcg ctggttgagg agagcctgtt ggaattgttc ctgctgg
//
LOCUS       DAOTON010000967         1076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_112249_length_1076_cov_3.401567, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000967 DAOTON010000000
VERSION     DAOTON010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112249_length_1076_cov_3.401567"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..556
                     /locus_tag="P5292_14260"
     CDS             293..556
                     /locus_tag="P5292_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018754457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRS40340.1"
                     /translation="MSTAYKNCQSCGMPLKKDPKGGGTNADGSRSTMYCSLCYANGKF
                     LQPDWTASQMQSFVREKMKEMGIPGFLAGFFSKGVPRLERWKK"
     gene            623..916
                     /locus_tag="P5292_14265"
     CDS             623..916
                     /locus_tag="P5292_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40341.1"
                     /translation="MTILNSLSRPDRHLAEGLLSSVIATLFLFFIDEGNYDFRWMASF
                     GNWVVFLLYVVGTLPLYWLLARILLRETDSLKKIPLISLAGAIALIAVFVLIK"
BASE COUNT          273 a          282 c          255 g          266 t
ORIGIN      
        1 gtgctcaccg accaacagac cgcaggttcg ggattcccgt tttctcctgc cttcgcttgt
       61 ttcgagcaga tcaaggcaag aagtctcaac aagaattgca ggcgaaagaa cggctggacg
      121 gctgagtaaa gcatcccgaa acctaatgaa accaatcctg cttcctgatt aaggaaaagt
      181 taaaagaact gtcaggaatg caaataggtg attgccgaaa aagcgcattg catttccggt
      241 aacaagactg caacttcgta gcaccttctg ccatcactaa aatccagcac cgatgtcgac
      301 cgcctacaag aattgtcaaa gctgcggaat gccattaaag aaagacccca agggtggggg
      361 taccaatgcc gacggatcgc gcagcacgat gtactgcagc ttgtgttatg cgaatggaaa
      421 attcctccaa cccgactgga ccgcgagtca gatgcagtcg ttcgtcaggg agaagatgaa
      481 agaaatgggt atccctggct tcctggcggg cttcttcagc aaaggtgtgc cgcggctgga
      541 gcgttggaaa aagtaacgcc ttccgtttca tgtttccaat agcggtaaga caataattag
      601 agtccgatcg cgttcagatc gaatgaccat cctcaactcc ctctcccgcc ctgaccgaca
      661 tcttgctgaa ggtttacttt cctccgtcat cgccacgctc ttcctcttct tcatcgacga
      721 agggaactat gactttcgct ggatggcgtc ctttggcaac tgggttgttt ttctgttgta
      781 cgtggtcggt acgcttccgc tatactggtt actggcccgc atcctgctgc gcgagaccga
      841 cagtctgaaa aaaataccgc tcatttcgct tgccggcgca attgctttga tcgcagtatt
      901 cgtgttgatt aaatagccgg aggcgattca aacacatatc cgctattccg gaacttagta
      961 ccgcacgaac aacatgatca acttcatcgc ctccgtattt ctattgtatt ccacggcaca
     1021 agattatcgg tttgataagc ctgaacaatc ggtcgtgtta cctccggtat tgcacg
//
LOCUS       DAOTON010000968         1076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_112300_length_1076_cov_2.236043, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000968 DAOTON010000000
VERSION     DAOTON010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112300_length_1076_cov_2.236043"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="P5292_14270"
     CDS             complement(<1..228)
                     /locus_tag="P5292_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40342.1"
                     /translation="MYSVKHTNFDSNRKSICLWHVMPLQWILLLFLFFGQFNNSFAQA
                     TVTASGTWSTATWSPAAPVAGQSVIVNVGCTL"
     gene            complement(529..>1076)
                     /locus_tag="P5292_14275"
     CDS             complement(529..>1076)
                     /locus_tag="P5292_14275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40343.1"
                     /translation="SNVTGLTTSTPSPCMGTDILVTWNATNGASTYDVLVPVGYTVLS
                     GTPTSNTFAIVNAGFSNGSIGIRANSSCGNSGTATLAITPVGCRVAGSADVAAALRTE
                     VFPNPTTGTVNIRFNSENSDMQYVVNVTDLSGRVILSERNKASKGSNLHTLDLSGMAK
                     GTYLVSLLTDNGKEVVRVTVE"
BASE COUNT          264 a          242 c          311 g          259 t
ORIGIN      
        1 cagcgtacaa cctacattca cgatcacgct ttgaccggca acaggcgctg caggactcca
       61 ggtcgccgtg gaccaggttc cgcttgctgt tacggtagct tgtgcaaatg aattgttgaa
      121 ttgtccgaaa aacaagaaga gcaacaggat ccactgcaag ggcataacat gccaaaggca
      181 gatcgacttg cgattactgt cgaagttggt gtgtttcact gagtacatcg gaaaagcggg
      241 ttgatggccc ttttccaagt tgagtaaaaa tagtttttcc aagcctgatt atcaatactt
      301 gttcataaga aatgcgcagt aaaccattct aaaatatcta atttttgggg aaaatttcaa
      361 tttttagggt agagactatc gagcatttgg ccaatggggg gaatctgggg atgaatcgtt
      421 ggcaattggc tccataatcg acgtaaaatg ggctatccca gactgcttgt tgggataaaa
      481 aaagccggtc ccgaaaggga ccggcttttt gattaatcgg gtataggatt attctaccgt
      541 gacgcgtaca acttccttac cgttatcggt gaggaggctg accaggtagg ttccttttgc
      601 cataccgctc aggtcgaggg tgtggaggtt ggaacctttg gaggccttgt tacgctcaga
      661 gagaataaca cgaccgctca ggtcagtgac gttcactacg tactgcatgt cgctgttttc
      721 gctgttgaag cggatgttca ccgtgccggt ggtcggattc gggaacactt cggtgcgaag
      781 agctgctgct acgtcagcag atcctgcaac acggcaacct accggggtaa tggccagggt
      841 ggccgtaccg gagttaccac agctgctgtt ggcacggatg ccaatggacc cgttggagaa
      901 gcccgcgttc acgatagcga aggtattcga ggtcggtgta cccgagagta ccgtgtaacc
      961 aaccggaacc agtacgtcgt acgtagaagc gccgttggtg gcgttccagg tcaccaggat
     1021 gtcggtgccc atacacggag acggagtgga ggtggtcagg ccggtgacgt tggacg
//
LOCUS       DAOTON010000969         1075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_112426_length_1075_cov_3.281373, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000969 DAOTON010000000
VERSION     DAOTON010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112426_length_1075_cov_3.281373"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="P5292_14280"
     CDS             complement(<1..1016)
                     /locus_tag="P5292_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40344.1"
                     /translation="MFIARHSIRVFSAFFVLTFSVIPACPASVLSEQEIKQDLIEILS
                     NLEYLIDANMQGGELPEQGSTVQSLTESQMTLRQVLQSADAIMVTGVDSLTPQDLYLL
                     KMLSMNLQIARELTDLKNQLASGAKQERSFVQVGKKKIASGPIRMNPLSSGDALPASD
                     TEFPKANYSPSVLGDFNDSRIETTIAAITTVFSSFLNDATGLLSNELLKDTNLGIFGN
                     LNTINPLNLINDFIQDLTFKIHMTENRHDIAPLYIDFYLASLYNGISDICNNITASFG
                     ISFGVSVVVKFDVGINANFICIGSKTLANMHQFVYAKRSFMDSDIDSAEIEGSYERLE
                     YIHR"
BASE COUNT          275 a          258 c          267 g          275 t
ORIGIN      
        1 tgccggtgga tgtattccag ccgctcgtag gagccttcga tctccgccga atcgatatca
       61 ctatccataa aagatcgttt ggcatataca aactggtgca tattcgccag ggttttgctc
      121 ccgatgcaga tgaaattggc gttgatgccg acatcaaatt taacgacaac acttactcca
      181 aaactgatac cgaacgaagc ggtaatgttg ttgcagatat cggagattcc attgtagaga
      241 gaggccaggt aaaaatcgat atacaagggc gcgatatcat gccggttttc agtcatatgg
      301 attttgaatg tcaaatcctg tatgaagtcg ttgattaaat tcagagggtt tatcgtattc
      361 aagttgccaa aaatgcccaa attggtgtct tttaacaatt cattgctaag taatcccgtg
      421 gcgtcattga gaaaagaaga aaataccgtt gtaatagccg cgatcgtggt ttcgatccta
      481 ctatcattga agtcccctaa cacggatggg ctgtagttcg ccttcggaaa ctccgtgtcg
      541 ctcgccggca aggcatctcc ggaacttaga gggttcatgc ggatggggcc tgaagcaatt
      601 ttctttttgc cgacttgcac aaacgatctc tcttgcttcg ctcccgaagc cagttggttt
      661 ttcaaatcgg tcaattcgcg ggcgatttgc aagttcatgc tcagcatctt cagcaagtac
      721 aggtcctgcg gggtcaggga atcgaccccg gtgaccatga tggcatcggc gctttgcagg
      781 acttgccgga gggtcatctg gctttcagtc aaggactgca cggtgctgcc ctgctccggc
      841 agttcaccac cttgcatgtt cgcgtcgatg aggtattcca ggttggacag gatctcgatc
      901 aaatcctgct tgatttcctg ctcactgagg acggaggccg gacaagccgg aatcacagaa
      961 aaggtcaaca caaaaaacgc agaaaatacc ctgattgaat gacgtgcgat aaacatgatt
     1021 ctactcctcc taccaagatg gactggcggt caaggccata cgccggaatc ccggc
//
LOCUS       DAOTON010000970         1074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_112595_length_1074_cov_3.044161, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000970 DAOTON010000000
VERSION     DAOTON010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112595_length_1074_cov_3.044161"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="P5292_14285"
     CDS             complement(<1..215)
                     /locus_tag="P5292_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRS40345.1"
                     /translation="MKKLTLLVLRSFAGPLVLTFLIALFVLEMQFLWKYIDDLVGKGL
                     DTWIIVQLMVYVSVTLVPLALPLDILLS"
     gene            254..844
                     /locus_tag="P5292_14290"
     CDS             254..844
                     /locus_tag="P5292_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40346.1"
                     /translation="MTLSQDDLHVLCDTRFFPARFRIAQELDQLLSRLKDALSDELKS
                     WPLPVEGVDTSRGKIFRGENYRQFPYHLLDYPRRFDAKGVFAMRSLCRWGNEWSFTLH
                     LQGEGLQPFREKLVPARDRLLKAEVWWCTNDTPWEYHFEKDNYRKLVEIRPDEFEHRC
                     REGSFVKLSRQLPLARTGEVVEFGQDTFRLFRQCLS"
     gene            complement(839..>1074)
                     /locus_tag="P5292_14295"
     CDS             complement(839..>1074)
                     /locus_tag="P5292_14295"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HRS40347.1"
                     /translation="EQRARIVARKENQMIRYKWITEDKSKDETFFQFEIQQDEITGDV
                     ALIVTDFASDDEKAGNMMLWDSQIHALMHHIGS"
BASE COUNT          260 a          273 c          299 g          242 t
ORIGIN      
        1 gaaagcaaaa tgtccagtgg aagcgcaagc ggtaccaggg taaccgacac gtataccatc
       61 aactggacga tgatccaggt atcgagtcct tttccgacca ggtcgtcgat gtacttccag
      121 aggaactgca tctccaggac gaacaacgcg atcaggaaag tcaggaccag cggaccggca
      181 aaggagcgga gaaccagcag tgtaagcttc ttcacgtatc tttgaagctg aaaagcccgc
      241 gcaagttaag aaaatgacct taagccagga tgacctgcac gtactctgcg atacccggtt
      301 cttcccggcc cggtttcgca tcgctcagga attggatcag ctcttgtcgc gcctcaaaga
      361 tgccttgtcg gacgaactga agagctggcc gctaccggtt gaaggtgtcg ataccagcag
      421 gggcaagatc tttcgcgggg aaaactaccg gcagttcccg tatcacctgc tggactaccc
      481 gaggcggttc gatgcgaagg gagtcttcgc gatgcgttcg ctttgtcgct ggggaaatga
      541 atggagtttt acgctgcacc tgcagggaga gggcctgcaa ccctttcggg agaaactggt
      601 ccctgcacgg gatcgtttac tgaaagcgga ggtttggtgg tgtaccaacg atacaccctg
      661 ggagtaccat ttcgaaaagg acaactaccg gaaattggtg gaaatccgac ccgatgaatt
      721 cgaacatcgg tgccgggaag gatcgtttgt caagttaagc agacaactgc cgctcgcgcg
      781 aaccggagaa gtggtggaat tcggacagga tacttttcgc ctcttcaggc aatgcttgtc
      841 atgaaccgat gtggtgcatg agggcgtgga tttgagaatc ccacagcatc atgttgcccg
      901 ccttttcatc atccgatgcg aaatcggtca cgatcaaggc tacatcaccg gtgatctcgt
      961 cctgttggat ctcaaactga aaaaaggtct cgtcctttga tttatcctcg gtgatccact
     1021 tgtaccggat catctgattt tcttttcgcg ctacgatgcg ggcacgctgt tccg
//
LOCUS       DAOTON010000971         1068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_113671_length_1068_cov_2.953603, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000971 DAOTON010000000
VERSION     DAOTON010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113671_length_1068_cov_2.953603"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="P5292_14300"
     CDS             <1..431
                     /locus_tag="P5292_14300"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRS40348.1"
                     /translation="MHCDDPPCVRNCPSGVFYKRDDGVVMYDNSKCYGARVCRMVCPY
                     EAISFIEKVEPYFPQGFTPIEKYWDNASKEGTAAKCDFCIDRVTSGLKPACVKTCPTE
                     ALKFGDLNDPDSEVSKLILDRGGYQMHAELGTNPSVYYLP"
     gene            442..>1068
                     /locus_tag="P5292_14305"
     CDS             442..>1068
                     /locus_tag="P5292_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40349.1"
                     /translation="MISNEATKRVEALSTYTRLIPESQEIGPQYKAQADWKFIEVLSF
                     YLGGVGTGLYLLSQVFDYAIGLIVGYLLVVVGKNGAHLLASSKPFRAIRVLSKPGTSW
                     ISRGAYGIFLFAVLGLVDIALRTGVVPSENAALAKVVTILTACAAFFIMAYVGFVFAE
                     ARNIGFWNSPIIPALIICYSFCMGYALLLYMAPLTPNQFNFPAISLQFL"
BASE COUNT          215 a          262 c          319 g          272 t
ORIGIN      
        1 gcatgcactg cgacgatccg ccgtgcgtga gaaactgccc gtcgggtgtt ttttacaagc
       61 gggacgacgg ggtggtgatg tacgacaaca gcaaatgtta tggcgccagg gtgtgccgca
      121 tggtctgccc ttatgaggcg atatcgttta tcgaaaaagt cgagccctat tttccgcagg
      181 gatttacacc gatcgagaaa tactgggata acgcatccaa ggagggaacg gccgcgaagt
      241 gtgacttctg tatcgaccgg gtgacgtccg gtctgaaacc ggcttgcgtg aaaacgtgtc
      301 cgacggaagc actgaagttc ggtgatctga acgatcccga cagtgaagtt tccaaactta
      361 tcctggaccg gggcgggtac cagatgcacg ctgaactggg tacgaacccg tcggtgtatt
      421 atcttccata ggaggtagac catgatatcg aatgaggcaa ccaaacgagt tgaagcgctg
      481 tcgacctata cgcggcttat tccggagagt caggagatcg gccctcagta taaggcacag
      541 gcggactgga aatttatcga ggttctttcc ttctatctgg gaggcgtcgg aacggggctt
      601 tacctgttgt cgcaggtatt tgactacgcg atcgggttga tcgtggggta tctgctggtc
      661 gttgtcggca agaacggggc ccacctgctg gcctcctcca agccgttccg ggcgatacgc
      721 gtgctgagca agccggggac ttcctggatc agccggggcg cgtacggcat ttttttgttt
      781 gccgttttag gtttggtgga cattgccctg cggaccggtg tcgttccgtc tgaaaatgca
      841 gcattggcca aggtggtgac gattctgacg gcctgtgcgg ctttcttcat catggcctat
      901 gtagggtttg tgtttgcgga agcgcgcaat attggtttct ggaattctcc gatcatcccg
      961 gcgctgatta tatgctactc tttctgcatg ggttacgccc ttctgctgta catggctccg
     1021 ttaacgccga atcaattcaa tttcccggca atttccctgc agttcctg
//
LOCUS       DAOTON010000972         1067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_113902_length_1067_cov_1.995059, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000972 DAOTON010000000
VERSION     DAOTON010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113902_length_1067_cov_1.995059"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1067
                     /locus_tag="P5292_14310"
     CDS             <1..>1067
                     /locus_tag="P5292_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40350.1"
                     /translation="TSMGGAFTAVGGDFSSLSQNPAGMAIYRRSELTFTPSIYAGKTS
                     STFLGNTADDSRYNFNIANAGFVFTQPLTKNDEGEGWKSWNFGIGLNRINNFHSRSFY
                     EGFNLDNSFTDYLAQQAQGVGYENLDGYYEYLGYYTYLINPDSLNNYTSAVPFGHVTQ
                     RRSSEKRGAITETAFSVSGNYSNKLFLGATLGFNSLRYTEESTYEELDEQNLVDSLKQ
                     FQFDQSLTTRGVGVNLKFGMIYWINEYVRFGAAVHTPTWYNMNDSYQNTMQSRFDGGY
                     TARKQSPSGTFDYYMTTPFRAMGGLAFVFNQYGLVSADYEFNDISTARISATGFSFAD
                     VNNEIRRKYTATNTVRIGTEW"
BASE COUNT          249 a          337 c          259 g          222 t
ORIGIN      
        1 cacgtcgatg ggtggcgcgt tcaccgctgt cggcggcgac ttcagcagcc tgtcacagaa
       61 ccccgcggga atggcgatct atcgtcgctc ggagctgacc tttactcctt cgatctacgc
      121 gggtaagaca tcttccacct ttctgggcaa cactgccgac gacagtcgtt acaacttcaa
      181 catcgccaac gccggcttcg tcttcacgca accgctgacc aagaacgacg aaggtgaggg
      241 ctggaaaagc tggaacttcg gtatcgggct gaaccggatc aacaacttcc acagtcgttc
      301 cttctacgaa ggcttcaacc tcgacaactc cttcaccgat tatctcgctc agcaggcgca
      361 gggcgtcggc tacgagaacc tcgatgggta ttacgaatac cttggctact atacgtatct
      421 catcaacccc gactcgctga acaattacac cagcgccgtt ccgttcgggc acgtgacaca
      481 gcgccgctct tctgaaaagc gtggcgccat cacggaaaca gcgttttcgg tcagtggcaa
      541 ctacagcaac aagctcttcc tcggcgccac gctcggtttc aattcgctcc ggtacaccga
      601 ggaatccacc tacgaagaac tggatgagca gaacctcgtc gacagcctca aacagttcca
      661 attcgatcaa agccttacca cccgcggtgt aggcgtcaac ctgaaattcg gtatgatcta
      721 ttggatcaac gaatacgtcc gctttggcgc cgcggtccat acacctacct ggtacaacat
      781 gaacgactcg taccagaaca ccatgcagtc gcgcttcgac ggtggctaca ccgcgcggaa
      841 gcaatctccc tccggaacgt tcgattatta catgaccact cctttccgtg cgatgggcgg
      901 tctcgccttc gtcttcaacc aatatggcct cgtaagcgcc gattacgagt tcaacgatat
      961 ctccaccgca cgcatctcgg cgaccggttt cagcttcgcc gacgtgaaca atgagattcg
     1021 gcggaagtac acggcgacga acacggtacg catcggtacc gaatggc
//
LOCUS       DAOTON010000973         1066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_114023_length_1066_cov_2.831850, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000973 DAOTON010000000
VERSION     DAOTON010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114023_length_1066_cov_2.831850"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="P5292_14315"
     CDS             complement(<1..787)
                     /locus_tag="P5292_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRS40351.1"
                     /translation="MILRERIFIQLLLVPAILVCSLAGWAQSPLFRQYELRESRLKPR
                     ILCMANDRQGLLWLGTEDGLFYFNGLEFKRPYLADSVKNLSVTALAQDRQGHMWAGTD
                     KGIILRLHHYQQEAFVPEEGFPKVAISGIVEERDGTLFFSTHGEGIYYWNGKRLYNVD
                     TDDGLSDNYCYSLVLGPGNGVIAGTDNGISHVTVRDSTKKINVIGRAEGLTDDIVRVI
                     CPVKGGYWIGFQEGGVLFLDLRLKTLSRTTGYPAGIPSQVNSLC"
     gene            complement(784..>1066)
                     /locus_tag="P5292_14320"
     CDS             complement(784..>1066)
                     /locus_tag="P5292_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRS40352.1"
                     /translation="EAIFTGKIIEDIDLATDGTYAVRAKGKLKIHGVEQERIIRAKIE
                     IRNGVLHLVSQFTVLLADHSIKVPKVVHEKIASEINITVRAELTIKAMK"
BASE COUNT          244 a          290 c          261 g          271 t
ORIGIN      
        1 cgcagagcga attgacctgg ctcggtatgc cggcgggata ccctgtagtt cgtgataaag
       61 ttttcaggcg aaggtcgagg aacaggacac ctccctcctg aaaaccgatc cagtatccgc
      121 ccttgaccgg acaaatgact ctgacgatat catcggtaag gccttcggcg cgtccgatta
      181 cgttgatttt cttcgtgctg tcacgtaccg tgacatggga aattccatta tccgtcccgg
      241 cgatcacacc gtttccaggt ccgagtacca gcgagtaaca gtaattgtca ctgaggccat
      301 catcggtatc cacattgtac agtctcttcc cgttccaata atagatccct tctccgtgtg
      361 tcgagaagaa aagggttcca tcgcgttcct ccacgattcc actgatggcg actttgggaa
      421 atccctcttc cgggacaaag gcttcttgct gatagtggtg tagtcgaagg atgattccct
      481 tatccgtccc cgcccacatg tgtccctgac gatcctgtgc aagagcggtc acggacagat
      541 ttttcacact gtcggccaga taggggcgct tgaattcaag cccgttgaaa tagaataagc
      601 cgtcttccgt acccaaccag agtaaaccct gtcggtcatt cgccatgcag agaatacggg
      661 gtttcaacct gctctcgcgt aattcgtatt gccggaagag cggcgattgt gcccaccccg
      721 caagactgca aaccaggatg gctggaacta acagaagctg aatgaaaatt ctttcgcgca
      781 ggatcatttc attgccttga tcgtcagttc tgcacgcacg gtgatgttga tctcggacgc
      841 gatcttttcg tgaaccacct tgggtacttt gatcgaatga tcggccaaca agacggtaaa
      901 ttgggaaacc aggtgcaaca cgccgttacg gatttcgatc ttcgcccgga tgatacgttc
      961 ctgctccaca ccgtggattt tcaattttcc tttcgcgcga acggcatacg ttccatcggt
     1021 agcgaggtcg atgtcttcga tgatcttacc cgtgaaaatt gcttcc
//
LOCUS       DAOTON010000974         1064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_114404_length_1064_cov_2.733399, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000974 DAOTON010000000
VERSION     DAOTON010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114404_length_1064_cov_2.733399"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="P5292_14325"
     CDS             complement(<1..961)
                     /locus_tag="P5292_14325"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRS40353.1"
                     /translation="MAIIRPFKGIRPKKELVEKIASKPYDVLNEKEARAEAAGNNLSF
                     YHVIKPEIDFPDGYNVYAPEIYTKGKSNFDRMVQDGVFFKDKTDCLYIYAQTMKGRRQ
                     FGIVAAAAVDDYFNDIIKKHELTRPDKEEDRKNHVRVSKLNYEPVFFAYPNVPALDKI
                     VAGVVQAPAEYDFTADDGIRHEFWVISDGATVNQMVDLFAGIPATYVADGHHRTAAAA
                     LIGQELRKAKPDYSGKESFNFFLAVHFPDNQLAIMDYNRVVKDLNGLSNEQFLDSLRK
                     SFLVEPVGAAAYRPARLHEFSMYLDGQWYKLTAKTGTYDDADPI"
BASE COUNT          250 a          242 c          287 g          285 t
ORIGIN      
        1 cgatcgggtc agcgtcatcg tacgttccgg ttttggcggt gagtttatac cattgtccgt
       61 cgaggtacat gctgaattcg tggagtcgcg caggacggta cgccgcggca ccgaccggtt
      121 cgaccaagaa tgatttacgg agcgaatcga ggaattgttc gttgctcaaa ccgttgagat
      181 ccttgacaac acggttgtaa tccatgatgg ccaactggtt gtcaggaaag tggacagcca
      241 gaaagaaatt gaacgattct ttgcctgaat aatccggttt cgcttttcgt aactcctgac
      301 caatcagggc agcggcagcg gtacggtggt gaccatctgc gacataggta gcgggtatcc
      361 cggcgaataa atcaaccatc tggttgaccg ttgcgccatc cgagatcacc cagaactcgt
      421 gacgaatacc atcgtcagct gtaaagtcgt attcggccgg agcttgaact acacccgcga
      481 cgatcttgtc gagcgcagga acattcgggt atgcgaagaa cacgggttcg tagttgagct
      541 tcgatacgcg aacgtggttc ttgcggtctt cctctttatc cggacgggtc agttcgtgct
      601 ttttgatgat atcgttgaag taatcatcca ccgcggcggc ggctacgatg ccaaactgcc
      661 ggcgaccctt catggtctgg gcatagatgt agaggcaatc ggtcttatcc ttgaagaaaa
      721 cgccatcctg aaccatccga tcgaagttcg atttgccttt ggtatagatc tctggcgcat
      781 acacattgta accgtcagga aaatcgatct ccggcttgat tacatggtag aaggacagat
      841 tgtttccggc ggcttctgca cgagcctcct tttcgttcaa aacgtcataa ggcttcgaag
      901 cgatcttctc aacaagttct ttcttcggac ggattccttt aaaggggcgg atgatagcca
      961 tggtggtggg tagttgattc gggtttcggg gactggtagc tggtaatttg taattagtaa
     1021 ttagtaatta gtaattagta attagttgta atagttagaa cttc
//
LOCUS       DAOTON010000975         1060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_115164_length_1060_cov_2.255721, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000975 DAOTON010000000
VERSION     DAOTON010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115164_length_1060_cov_2.255721"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1060)
                     /locus_tag="P5292_14330"
     CDS             complement(<1..>1060)
                     /locus_tag="P5292_14330"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRS40354.1"
                     /translation="AHPWGSTNQPGVACGDDNVSDTPETQGWTTCNINGSVCNPPIIE
                     NVQNHMDYSYCTQMFTAGQAARMQAALNSAIGGRNNLWSPSNLAATGALASPAPTCTP
                     NADFYSSDQTACEGVALTFYDLSWNSPVSSRVWDFPGGSPSTSTDSSVVVTYPAAGVY
                     GVTLTVTNSAGSASVTKTNFVRISGAAQTVAPVTEDFSLAPSFPGVDGYVVNQDNGQT
                     WNRVTTVGASGSESVRIINYTNISGQIDDWITPSYDLSNMTGISLLFKVANARRNSSS
                     TDQLKVDYSLNCGETWQPRYNKSGAALATAGVVSVSFVPNATQWRQETVSLPTSVNLK
                     PTVRFRFRNVSDRGNNTYI"
BASE COUNT          208 a          281 c          347 g          224 t
ORIGIN      
        1 gatgtaggtg ttgttgcccc ggtcgcttac gttgcggaag cggaaacgga cagtcggttt
       61 caggttgacg gaagtcggca gcgaaacggt ttcctgacgc cattgcgtgg cgttcggtac
      121 gaagctcaca cttaccacac cagcggtagc gagggctgca cccgacttgt tataacgcgg
      181 ctgccaggtt tcgccacagt tcagactgta atcgactttg agctgatcgg tggaagagct
      241 gttgcggcgg gcattggcta ctttgaagag caacgagatg ccggtcatgt tcgagaggtc
      301 gtaggagggt gtgatccagt cgtcgatctg accggagatg ttggtgtagt tgatgatacg
      361 cacgctctcc gacccgctcg caccaaccgt cgttacccgg ttccaggtct ggccgttgtc
      421 ctggttgacc acatatccgt caacgccggg gaaactcgga gcgaggctga agtcttccgt
      481 cacaggagct acggtttgcg ccgcgccgct gatgcgaacg aagttggtct tggtgaccga
      541 ggccgatcct gcactgttgg taacagtcag ggtgacgcca taaacgccgg cagcggggta
      601 cgtgaccacc accgaagaat ctgtcgaggt actgggggag ccgcccggga agtcccatac
      661 acgggagctc accggactgt tccaggagag gtcgtaaaag gtgagggcaa cgccttcaca
      721 agcggtctgg tcgctcgaat agaaatcggc attcggcgta caggtcggtg caggagaagc
      781 cagcgccccg gtagccgcca ggttggaagg actccagagg ttgttgcgtc cgccgatggc
      841 gctgttcagc gcggcctgca tgcgtgcagc ctgtccggca gtgaacatct gtgtgcagta
      901 cgaatagtcc atgtggttct gcacgttctc gatgatcgga ggattacaga ctgaaccgtt
      961 gatgttgcag gtcgtccaac cctgagtctc cggggtgtcg gaaacgttgt catcaccaca
     1021 agcgacgccc ggctggttgg tgcttcccca tgggtgcgcg
//
LOCUS       DAOTON010000976         1057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_115718_length_1057_cov_2.173653, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000976 DAOTON010000000
VERSION     DAOTON010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115718_length_1057_cov_2.173653"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="P5292_14335"
     CDS             complement(<1..233)
                     /locus_tag="P5292_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40355.1"
                     /translation="MRKPRVPELATVILYQFEKFSASTHPPPPSETMPGPVLASLKET
                     SLQIGEGERQSLLSVPFGPFAQGTQSRVTTQPAR"
     gene            226..>1057
                     /locus_tag="P5292_14340"
     CDS             226..>1057
                     /locus_tag="P5292_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40356.1"
                     /translation="MRIVPNVAADDYDFALYQATSCAGLGSPVRCSYAANTGNTGMDN
                     TLNLTTNTAICGPANNGSDISEDVCGNGWVNDLAVTTGQNYYLLVNKWTPGGSGFTLD
                     WVLAGGATLNCSVLPIELLNFTAEAKNDRVDLQWSTSSETNNSYFTVERSQDARTFEP
                     IQIVPGAGNSSSERYYLSSDFEPLPGISYYRLRQTDFDGKFTFSEPVAVRYSNTVKSL
                     ALVPNPANDRVRVIFESGVEAGGTLRVFSLEGKLMQQQSLQMRRGTNSLDLDLTDWKS
                     G"
BASE COUNT          236 a          294 c          293 g          234 t
ORIGIN      
        1 cgggccggtt gggtggtgac cctagactgt gttccctgcg cgaacggacc gaacggcacc
       61 gataacagcg actgccgttc accttctccg atctgtagcg acgtttcctt cagtgacgca
      121 agtacggggc ccggcatcgt ctccgatggc ggcggcggat gtgtgctcgc ggagaatttt
      181 tcgaactggt acaagatcac cgttgccaat tccggaaccc tgggcttgcg catcgttcct
      241 aacgttgcag ccgacgatta cgactttgcg ctttaccagg caacatcctg tgccggactc
      301 ggaagcccgg tgcgctgctc ttatgccgcc aataccggta atacaggaat ggataacacg
      361 cttaatctta cgaccaatac ggcgatttgt ggccctgcta acaacggttc cgacatatcg
      421 gaggatgtat gcggtaacgg ctgggtgaat gacctggccg tgaccaccgg acagaactat
      481 tatctgctgg tcaacaagtg gacccccggc ggtagcggct ttactttgga ctgggtgctc
      541 gcgggtggtg caacactcaa ttgcagtgta cttcccatcg agttgctgaa ctttacagcg
      601 gaggcgaaga acgatcgcgt cgacctgcaa tggtcgacca gctcggaaac caataacagc
      661 tactttactg tggaacgcag ccaggatgcc cgcacattcg aaccgattca gattgtgccg
      721 ggggcaggca acagttcctc ggaacgttat tacctgagtt ccgatttcga gccgctacca
      781 ggtatcagtt actaccgcct gaggcaaact gatttcgatg gcaagttcac cttttccgaa
      841 ccggtcgcgg tgcgctattc caataccgtg aaatcactag cactggtacc caatccggct
      901 aacgatcgtg ttcgggtgat cttcgaaagc ggagtggaag caggcggaac gctccgcgtc
      961 ttctcactcg aagggaaatt aatgcagcag caatcactcc agatgcgccg cgggaccaat
     1021 tccctcgatc tggatttgac cgattggaaa agcggca
//
LOCUS       DAOTON010000977         1052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_116563_length_1052_cov_3.406219, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000977 DAOTON010000000
VERSION     DAOTON010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116563_length_1052_cov_3.406219"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..>1052
                     /locus_tag="P5292_14345"
     CDS             26..>1052
                     /locus_tag="P5292_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRS40357.1"
                     /translation="MIILAALLTAAAWPSMAVNPVYMGTLPAQVNESSGLSFTGGSSF
                     WTHNDGYGDNNLYKVSNTGTLSRTVTVLNAVNDDWEDLCHDVSRTWLYIGDFGNNDCD
                     RTNLRIYRVPYPSYVSGTTVTAEEISFTFPDQHAFPSPWMNFDVEAFFHFNGHLYLFT
                     KADGDAIGYTKMYRLSDDPGSYSATLVDSFYTNDRTTSADISPDGSSVILIANTRIHL
                     FRGFSGDDFFGGQHTLISINGTWAQREAVSFWSNNEIYLSNEDAGDGNELDYIDLSPW
                     IPAMTTGLSHINESVDFSVYPNPARNFLQANVGNINGSLLFELYDLTGKAVRTLRRES
                     ATEQLRMD"
BASE COUNT          223 a          337 c          270 g          222 t
ORIGIN      
        1 taacatccta cgcaacatac tccggttgat catcctagcc gctctgctca cggcagcggc
       61 ctggccgtcg atggcggtca acccggttta catgggtacg cttcctgccc aggtgaatga
      121 gtcctccgga ctttcgttca ccggcggttc cagcttctgg acgcacaacg acgggtacgg
      181 cgataacaac ctctacaagg tctccaatac cggaacgctt tcacgcaccg tcaccgtgct
      241 gaacgcggtg aacgacgatt gggaagacct ctgccacgat gtttcgcgca cctggttgta
      301 catcggtgat ttcggtaata acgattgtga ccggaccaac ctccggatct accgggtacc
      361 gtatccgtcc tatgtctctg gtacgacggt aacggcggag gagatcagct tcacctttcc
      421 ggaccagcat gcgttcccgt ctccctggat gaactttgat gtggaagcct tctttcattt
      481 caacggccac ctgtacctgt tcaccaaagc ggacggcgac gcgatcggtt acacgaagat
      541 gtaccgcttg tccgacgatc ccggctcgta tagcgcgacg cttgtcgaca gcttttacac
      601 caacgaccgc acgacctcgg cggatatcag tcccgacggc agcagcgtca ttctcattgc
      661 caacacgcgt atccacctgt tccgcggttt cagtggcgat gatttcttcg gcggtcagca
      721 taccctgatc agcatcaacg gcacctgggc acagcgcgag gccgtcagct tctggagcaa
      781 caacgagatt tatctctcga atgaagacgc cggcgacggc aacgaactcg attacatcga
      841 cctcagtccg tggatcccgg cgatgaccac cggcttgtcg cacatcaacg agagcgtcga
      901 cttctcggtc tatcccaatc ctgcgcgcaa cttcctccag gcgaacgtcg ggaacatcaa
      961 cggcagcctg ttgttcgaac tctacgacct cacgggcaaa gccgtgcgca cccttcgccg
     1021 cgaaagcgct acggaacaac tccgcatgga cg
//
LOCUS       DAOTON010000978         1052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_116567_length_1052_cov_3.278837, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000978 DAOTON010000000
VERSION     DAOTON010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116567_length_1052_cov_3.278837"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="P5292_14350"
     CDS             complement(<1..938)
                     /locus_tag="P5292_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRS40358.1"
                     /translation="MVQYWNGAYGLDYRIYPDSIVNINGSDTSRYFWRFSSTGLGRFD
                     AWSYDMVFDNLPDSISYPDIRNYRKPDTDQTLVSSFTCSDKVITVGSYANRNYYTNAN
                     FATTMDTSIVVGKLSSFSSKGPTRDGRIKPDITATGEWVLSCGTQAELNQLVAVEPEK
                     VAAGRKHKRSSGTSMSSPVVAGIAALYLQKNPSADWQEVKNALLRCADQDQYTGNNLP
                     DNDWGYGKVDAYAVVRGCTVGIEETGDSPYIDFGVYPNPASASTTFHFDLSLLRDNRE
                     PRIRITDPVGRTIKSFVLNRAAGTLEAAITDLAPGY"
BASE COUNT          206 a          269 c          324 g          253 t
ORIGIN      
        1 tagtaccccg gtgccagatc cgtgatggca gcctccagcg tgcctgcggc tctgttcaat
       61 acgaatgatt tgatggtccg gccgaccgga tcggtgatac ggatcctcgg ttcgcgatta
      121 tcacgcagta ggctgaggtc gaaatgaaag gtggtggatg ctgaagcggg gttggggtat
      181 acgccaaagt cgatatacgg actgtcgccg gtttcctcaa tgccaaccgt acatccgcga
      241 acgactgcgt aggcgtccac tttcccgtag ccccagtcgt tgtctggaag attgtttccg
      301 gtgtattgat cctggtctgc gcaacggagc aaggcgtttt tcacttcctg ccaatcggct
      361 gacgggttct tttggagata gagggcggca atgcctgcta cgaccggtga cgacatggac
      421 gtgccgctgc ttcgtttgtg cttgcgacct gcagcgacct tctcgggttc aacggctacg
      481 agctgattca gctctgcctg tgtgccgcac gataagaccc attcgccggt agcggtgatg
      541 tcgggcttta tgcgaccatc gcgtgttggt cctttgcttg aaaaggagga gagtttgccc
      601 acgacgatgg acgtgtccat ggtagtggcg aagttggcgt tggtgtaata attacgattg
      661 gcgtagctgc ctaccgtgat caccttatcg ctgcaggtga aactgctcac cagggtttgg
      721 tcggtgtccg gcttgcggta gttgcggatg tccggatagc tgatcgagtc gggcaggtta
      781 tcgaaaacca tgtcgtagct ccaggcgtcg aatcggccca ggccggtgga actgaatcgc
      841 cagaagtagc gggacgtatc ggaaccgttg atgttgacga tcgaatcagg atagatccgg
      901 taatccagtc catacgcgcc attccagtat tgcaccatgc tttcgatcag ccccagttga
      961 tttccctgcg cgtcaaacag ggtgtcggtc ttcaatactc cgggatcaag atcaccggct
     1021 cggaagggaa gctccgccag ggaagcgcgg gt
//
LOCUS       DAOTON010000979         1043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_118190_length_1043_cov_2.754049, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000979 DAOTON010000000
VERSION     DAOTON010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118190_length_1043_cov_2.754049"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1043)
                     /locus_tag="P5292_14355"
     CDS             complement(<1..>1043)
                     /locus_tag="P5292_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40359.1"
                     /translation="TLEGNATVTANISLDTTNVGAVPAGALLTLNGTIDGNTADTRQL
                     TLRCGQQTLSVSNAIGSTNRFSVLTIRADDVTSAASVRAQTINLYTGTPATRSMLIGD
                     AAGGVFSLNDAELALLTGFSTLTIGENGQQAASITLRTVVSPVSAAAVLVYANNGAGG
                     IVLDDEGTGTAFATGGAGNVTLVAGTGGITAASATNAFAELSTSGAVSLTSAGSIGSA
                     ANRIQFPAGQTNVVVNNAPNGVWFDGLGTLTLASITTNSTGASGLDVSTAGLLTLSGA
                     VSTSTGNITFNPSGNGVTANNTINGNSLTITNGGAVDLNGAITAPGGFSSTGTSFDNT
                     GATITTTNNAIVI"
BASE COUNT          227 a          287 c          273 g          256 t
ORIGIN      
        1 tgatgacaat tgcgttgttt gtcgtcgtga tcgtcgcacc ggtgttgtca aagctcgttc
       61 ccgtcgaact aaagccaccg ggcgctgtga tggctccatt gaggtcgaca gcaccaccgt
      121 ttgtaatagt aagtgaattt ccattaatgg tattattcgc cgttactcca ttgccactag
      181 gattaaaggt tatgttcccg gtggatgtcg atactgcgcc ggacaaagtc aaaaggcctg
      241 cagtgcttac gtcaagtccg gacgctccgg tcgagttcgt ggttatcgaa gctaaggtaa
      301 gcgtgccgag gccatcgaac caaaccccgt taggtgcatt atttaccacg acattggtct
      361 gtcctgcggg aaactgaatc cggttcgcag ccgagccgat actccccgcg cttgtcaggc
      421 ttacagcacc actcgtcgaa agttctgcaa aagcgttggt tgctgaggca gctgttatgc
      481 ccccggttcc tgcaaccagt gtgacgtttc cagccccgcc agtcgcgaat gccgtgcccg
      541 tgccttcgtc atcaagaacg ataccgccag cgccgttgtt ggcgtacact aataccgctg
      601 cggctgatac cggacttacc acggtccgca aggtgattga agcagcctgc tgtccgtttt
      661 ctccgattgt caatgtcgaa aaccctgtaa gcaaggcaag ttcagcatcg tttagggaga
      721 atacaccacc cgcagcatcg cctatcagca tggaccttgt agctggtgtt cctgtgtaca
      781 ggttgatagt ctgagccctc acactggctg cactagtaac atcatcagcg cgaatagtaa
      841 gtaccgaaaa cctattcgtc gaaccaatcg catttgatac cgagagggtc tgttgtccac
      901 aacgcagcgt caactggcgc gtgtcagcgg tattcccgtc gatagtgcca ttcagcgtga
      961 gtagtgcgcc tgccggcacc gctcctacgt ttgtggtatc cagactgatg ttggcagtaa
     1021 ccgtggcatt gccctcaagg gta
//
LOCUS       DAOTON010000980         1041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_118635_length_1041_cov_2.483773, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000980 DAOTON010000000
VERSION     DAOTON010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118635_length_1041_cov_2.483773"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1041
                     /locus_tag="P5292_14360"
     CDS             <1..>1041
                     /locus_tag="P5292_14360"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40360.1"
                     /translation="GANATSLTAGNYTVTATDANGCTVSSTFLISEPTLLSPLASSTD
                     ALCSGANNGTASVATNGGTPGYTYSWTPSGGTGANATGLGAGVYQVVVTDANGCTASA
                     TAIISEPSVLVLNVAGTSDVSCFNGADGTGSVTPTGGTANYSYAWSPSGGTGSSANGL
                     SAGSYTVTVTDQNGCTAQVPLTINQPTQLTVQASAIDARCNGSTDGSISAIANGGTGP
                     YGYTWSPGGAVTAGVNNVGAGNYSVTITDANGCTTTGSATVAQPAALNASATPVAVTC
                     NGGSNGTITVNVNGGTTGYSYSWFPGGATTANASGLSAGAYSVTITDANGCTTTAAAT
                     VTEPLAMQLSLSS"
BASE COUNT          235 a          296 c          292 g          218 t
ORIGIN      
        1 cggtgcgaat gctacaagtc tcaccgctgg taattataca gtcaccgcaa cggatgccaa
       61 tggctgcacc gtcagcagca cgttcttgat cagcgaaccg acgctcttgt caccgctggc
      121 aagcagcacc gatgccttat gcagtggcgc aaacaacgga actgccagtg tcgctaccaa
      181 tggcggcact cccggatata cctattcctg gactccatca ggcggaacgg gggcgaacgc
      241 gaccgggctt ggagcaggtg tttatcaggt cgtggtaacg gatgcaaacg gttgtactgc
      301 ttccgccaca gccatcatca gcgaaccgtc cgtcctggtt ttaaatgtcg cagggacctc
      361 ggatgtgagt tgtttcaatg gagctgacgg aaccggatcg gtgacgccca ccggcggaac
      421 cgccaactat agttatgcct ggtcaccgtc tggtggcact ggaagttcgg cgaacggact
      481 ttcagccggt agttacacgg taacggtcac cgatcaaaac ggatgcaccg cgcaggttcc
      541 gctcacgatc aatcaaccca cgcaactgac cgtacaagcc agcgccatcg atgcacgctg
      601 taacggatcc acggatggca gtatcagtgc gattgccaat ggaggaaccg gaccttatgg
      661 ttatacctgg agtcctggtg gagctgttac ggcgggtgtt aacaatgttg gagccgggaa
      721 ctattccgtt acgattaccg atgccaatgg ttgtacaact accggctcag cgaccgttgc
      781 acaaccggcc gcgcttaacg cttctgccac acctgtggct gttacctgca atggagggtc
      841 gaatggtacg attaccgtga atgtgaacgg tggtacaaca ggttattcct attcgtggtt
      901 cccgggaggt gccaccacgg cgaatgcatc gggcctttcg gcaggagcct acagtgtcac
      961 gatcacggat gccaacggct gtaccacgac tgcagccgca acggtcaccg aaccgttagc
     1021 gatgcaactc tccttatctt c
//
LOCUS       DAOTON010000981         1032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_120355_length_1032_cov_3.363357, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000981 DAOTON010000000
VERSION     DAOTON010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120355_length_1032_cov_3.363357"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="P5292_14365"
     CDS             <1..914
                     /locus_tag="P5292_14365"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRS40361.1"
                     /translation="SFHYRIRSAARAAGILLLLLVCLQRSAAQEQRGVMGIGISAGGT
                     NYIGELDDNFTLVFTRLGAGAHVNFLFFSRVHIRLAAFHGRVTADDARGTFSGNESRN
                     LNFFSDIDEAGIHVLYKLESRNKGFSKRNRIAPYLFAGLGYFRFDPKTTRNGVTYRLQ
                     KVGTEGQYLSSGNYPDPYKLQQFCIPMGIGFQVKITSNFDFGVETGFRRLFTDYLDDV
                     SGRYPDKAQMLQEQGPVALYLSDPSNDPDLPNGKASFSKRGNPKAMDWYVYTNAHLTY
                     YFTTALFGSYKLKNQFRDGSCKGLLRR"
BASE COUNT          231 a          288 c          272 g          241 t
ORIGIN      
        1 tgtcgttcca ttaccgcatc cggtcagccg cgcgtgccgc agggatcctc ctgctgttgc
       61 tggtatgctt acaacgaagc gcagctcagg aacagcgcgg cgtgatgggc atcggcatct
      121 cggccggcgg cacgaattac atcggcgaac tggacgacaa cttcacgctg gtgtttaccc
      181 ggctcggtgc cggtgcgcac gtcaacttcc ttttcttctc gcgcgtacac attcgcctgg
      241 ccgctttcca tggacgggtg acggccgacg acgcgcgcgg tacgttttcc ggaaacgaat
      301 cccgcaacct gaatttcttc agcgacatcg acgaggccgg catccacgtc ctgtacaaac
      361 tcgaatcgcg caacaaaggc ttttcgaagc gcaaccggat cgcgccctac cttttcgccg
      421 gtctcggcta cttccgtttc gatccgaaga ccacgcgcaa cggcgtcacg taccggttac
      481 agaaagtcgg taccgaaggc cagtacctct cttcgggcaa ctatcccgat ccgtacaagc
      541 tgcagcagtt ctgcattccg atgggcatcg gtttccaggt gaagatcacg tccaacttcg
      601 acttcggtgt ggagacgggc tttcgccggc tgtttacgga ttacctcgac gacgtcagcg
      661 gtcgctatcc cgacaaagcg caaatgttac aggaacaagg acccgtcgcg ctctaccttt
      721 ccgacccttc caacgaccct gatctgccaa acggaaaagc cagcttctcc aaacgcggca
      781 accccaaggc gatggactgg tacgtgtaca ccaatgcgca tctcacctat tatttcacca
      841 cagcactctt cggttcctat aaattgaaaa atcagttccg ggacgggagt tgtaaggggt
      901 tgttgaggag gtgaatgtgg tgattggtga ttggtgattg gtgattggtg actagtaatt
      961 ggtaattggt aattggtaat tagtaattag taactagaaa ctggtaacta gaaattggta
     1021 attactaatt ag
//
LOCUS       DAOTON010000982         1029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_120970_length_1029_cov_3.194045, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000982 DAOTON010000000
VERSION     DAOTON010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120970_length_1029_cov_3.194045"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1029
                     /gene="nadE"
                     /locus_tag="P5292_14370"
     CDS             <1..>1029
                     /gene="nadE"
                     /locus_tag="P5292_14370"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRS40362.1"
                     /translation="YVNHVGAQTEIIFDGGSLAMDRTGTIVSEAAYFEEDLLLVSLEN
                     DGQLTATKATDITRSAPKEKIERIYRALVLGIRDYFRKQGFRTATLGLSGGIDSAVVT
                     CLAADALGPENVKVLLMPSGFSSEGSVTDSLELIRANGVNHETIPIQSIFDAYLAALQ
                     PAFSGLPFNVAEENIQARIRGGLLMAQSNKFGSILLNTSNKSEIAVGYGTLYGDLCGG
                     ISVIGDVYKTDVFKLARFLNRDQARIPEAIITKAPSAELRPNQKDSDSLPEYDVLDPI
                     LFQYIEKRMSPSALVEMGNDPALVARILKLVNTNEYKRYQTPPILRVSPKAFGMGRRL
                     PIVGKYLS"
BASE COUNT          261 a          294 c          251 g          223 t
ORIGIN      
        1 gtatgtcaat cacgtcggag cacagacgga gatcatcttc gacggcggct cgctggcaat
       61 ggatcggaca ggaaccatcg tttcggaagc agcctacttc gaagaagacc tgctgttggt
      121 ttcacttgaa aacgatgggc aactcacggc aactaaagca accgacataa cacggtcagc
      181 cccgaaggag aagatcgaac gcatctaccg ggccctggta ctgggtatac gcgattattt
      241 caggaaacaa ggttttcgaa ctgctacgct gggactttcg ggcggcattg attccgctgt
      301 cgtgacctgc cttgcagccg atgcccttgg tcccgaaaac gtgaaagtac tgctgatgcc
      361 ttccggattc tcatcagaag gatcggtcac cgattccctc gaattgatcc gggctaacgg
      421 cgtgaaccac gaaacgattc ccatccaatc gatctttgat gcttatctgg ccgcactcca
      481 accggccttt tccggattac cgttcaatgt cgcggaagaa aacatccagg cgcgcatacg
      541 cggcggatta ctcatggccc aatccaataa gttcggttcg atcctgctga atacttccaa
      601 caagagtgag atcgcggtag gatacggaac actttacgga gatctgtgcg gcggaatatc
      661 ggtcatcggc gacgtctata aaacggacgt cttcaagctc gcccgtttcc tcaaccgcga
      721 tcaagcacgc attccggaag caatcattac caaagccccc tcggcggaat tacgtcccaa
      781 ccagaaagac agtgactcgc ttcccgaata cgacgtcctc gatcccattc tcttccaata
      841 catcgaaaag cgaatgagtc cctcagcttt ggttgagatg gggaacgatc ccgccctggt
      901 agcccgcatc ctcaagctgg tcaacaccaa cgaatacaaa cgctaccaga caccacccat
      961 tttacgggtc tccccgaaag cttttggcat gggaagacgg ttgccgatcg tcggtaagta
     1021 cttgtcata
//
LOCUS       DAOTON010000983         1026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_121757_length_1026_cov_1.622039, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000983 DAOTON010000000
VERSION     DAOTON010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121757_length_1026_cov_1.622039"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1026)
                     /locus_tag="P5292_14375"
     CDS             complement(<1..>1026)
                     /locus_tag="P5292_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40363.1"
                     /translation="GTGTDLTVNGTLRNAGTINMGGSVTASFGSTGTYIHNQNGGTIP
                     TATWNAASNCRLTGITNSYPSGAGQTFGHFTFNSPLVTDVEMSSNLTTAGNLNIFGTG
                     TRTLFLTNSSNNRTITVGANFNDSAGTFTAVNNSGTSTISVAGNFNLAGGTFNMKNGN
                     GTSAMSVTGDMNFTSGTMRLRSTSTVNNATLTLARHFTMSGGTLDFTSVAANSTMNVA
                     GNFSHTGGTMTESSTGVGSIVFNRSGTQVVTSGGTVSNAINYTVNSGSTLQLGTQLLG
                     SGSTGTFTLSSGGGLILGDPSGITSAENAGNIRVSGTRTYSTGGSYTYNGASAQQTGS
                     GLPATVAN"
BASE COUNT          189 a          259 c          340 g          238 t
ORIGIN      
        1 gttggcaacc gtcgccggta agccgcttcc agtctgttgc gcggaggcgc cgttgtaggt
       61 atagcttccg ccggtgctgt aggtgcgggt gccgcttacg cgaatgttac cggcgttttc
      121 agcggacgtg atcccggatg ggtcacctag aatcagtccg cctccggagg agagtgtgaa
      181 cgtcccggta ctgccgcttc caagcaattg cgtaccgagt tgtagcgtag aaccgctgtt
      241 gacggtatag ttgatcgcgt tgctcacggt gccaccgctg gtcaccactt gtgtgccgga
      301 tcggttgaag acgatggaac cgacgccggt tgaactctcg gtcatcgtgc cgccggtatg
      361 ggagaagttg ccggccacgt tcatcgtact gttggccgct acggaggtga agtccagcgt
      421 acccccgctc atggtgaaat gtctcgccag ggtaagcgtg gcattgttga cggtactggt
      481 cgagcgcagg cgcatcgtgc cggaggtgaa attcatatcg ccggtcacgg acatagccga
      541 agtgccgttg ccgtttttca tgttgaaggt cccgccggcc agattgaaat tccctgctac
      601 cgagatggta gaagtaccgg aattgttcac ggcagtaaag gtgccggcgc tgtcgttgaa
      661 gttcgcaccg acggtgatcg tgcggttgtt cgaggagttg gtgagaaata gggtgcgggt
      721 gcctgtgccg aaaatgttca ggttgccggc ggtggtgagg ttggagctca tctccacgtc
      781 ggtgacgagc ggactgttaa acgtaaaatg cccgaacgtc tgaccggctc cggaagggta
      841 ggagttggta atgccggtga gacggcagtt cgaagcggcg ttccaggtag cggtcggtat
      901 ggtgccgcca ttctggttgt ggatataagt acccgtgctt ccgaacgaag ccgtaacact
      961 accgcccatg ttgatcgtac cggcatttcg aagcgtaccg ttgactgtca ggtctgtgcc
     1021 ggttcc
//
LOCUS       DAOTON010000984         1025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_121943_length_1025_cov_1.609278, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000984 DAOTON010000000
VERSION     DAOTON010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121943_length_1025_cov_1.609278"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1025
                     /locus_tag="P5292_14380"
     CDS             <1..>1025
                     /locus_tag="P5292_14380"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRS40364.1"
                     /translation="AGGGTPVYAVTWNVPGQSGLVLDSMSAGNYSATLTDANGCTITT
                     SATIAAPDSITLQVIATNPRCYGSPDGSLIALAGGGTGAITYVWSPGTVDRDSLLDVP
                     AGTYVLLLTDANDCTRLDTFVIAQPDSIALQPLATPITCTGSRDGAVEVHPAGGTPGY
                     QFQWMPGNATDSVVTNLDTGSYRVVVTDANGCRDSVDVFVNDANPVSLVVAGPPVICT
                     GQTATLLATAIGTRPPYTFSWSDGSTLDSLVVQPSDTTSYEVYVTDSAGCRGEPVEIT
                     VNVYPPLDLSLDALPAICAGDSVLLHAVAGGGNGGPYSYNWNNGAVLTDEAMAYPVTD
                     SSFVVRV"
BASE COUNT          187 a          324 c          297 g          217 t
ORIGIN      
        1 agccggtggc ggaacacctg tatatgccgt tacctggaac gtgcccggtc aatccgggct
       61 ggtcctcgac agcatgtcag caggcaacta ttccgcgaca ctgacggatg ccaacggttg
      121 tacaataacg accagcgcca cgatcgcagc gcctgattca atcaccctgc aagtcatcgc
      181 aaccaatccg cgttgctacg gaagcccgga cggttcgctg atcgcgctcg ccggcggcgg
      241 tacgggagcg atcacctatg tatggtcgcc cggaacggtc gatcgcgatt ccttgcttga
      301 tgttccggcc ggcacgtatg tactcctgct caccgacgcg aacgactgta cccgtcttga
      361 taccttcgtt atcgcgcaac ccgattcgat cgcgcttcag ccactggcca cgccgatcac
      421 ctgcacgggt tcccgtgacg gtgcggtgga agtacatcca gccggtggta cgcccggcta
      481 tcagttccaa tggatgcccg gcaacgcgac ggattccgtc gtgacgaacc tcgataccgg
      541 ttcgtaccgc gtcgtagtca ctgacgcgaa tggttgtcgg gattccgttg atgttttcgt
      601 taacgatgcc aacccggtca gtctggtcgt agcaggcccg ccggtgatct gcaccggaca
      661 aacggctacg ctgcttgcca cggcgatcgg tacgcgtccg ccctacacgt tctcctggag
      721 cgacggttcc acgctcgaca gtctggtcgt gcaaccttcg gatacgacaa gctatgaggt
      781 ctatgtcact gacagcgccg gctgtcgcgg cgaaccggtg gagatcacgg tgaatgtgta
      841 tccgccgctc gacttgtcgc tcgacgcctt acccgccatt tgtgccggtg attccgtctt
      901 gttgcatgcg gtggccggcg gaggcaatgg cggcccgtat tcctacaact ggaacaacgg
      961 cgcggtcctc acggacgaag ccatggccta tccggtaaca gactcctcct tcgtcgtgag
     1021 ggttt
//
LOCUS       DAOTON010000985         1023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_122232_length_1023_cov_2.189050, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000985 DAOTON010000000
VERSION     DAOTON010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122232_length_1023_cov_2.189050"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="P5292_14385"
     CDS             complement(<1..192)
                     /locus_tag="P5292_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40365.1"
                     /translation="MRRLFFLTLLSLLFHLSAQSQPLQFQATRTHDNGSMGSILLAAP
                     DQGSVLAGHSALGNTGYLDL"
     gene            complement(204..875)
                     /gene="ccsA"
                     /locus_tag="P5292_14390"
     CDS             complement(204..875)
                     /gene="ccsA"
                     /locus_tag="P5292_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRS40366.1"
                     /translation="MKIAGLAWWKWLSLLLVLYAIVGGMLIPVPRLAILHETIRNLFY
                     HVTMWFSMMGLMMAALIFGIRYLRKGNLDDDLRADECVRTGVVVGILGLITGSIWARN
                     TWGAWWVNDAKLNGAAATMLVYFAYLILRGSMEDEHKRARITAVYSIFAFSLMIVFIM
                     ILPRMTDSLHPGNGGNPGFSSYDLDSRMRTIFYPAVLGWGLLMAWITEVRIRMKRLIR
                     EKEEL"
BASE COUNT          247 a          266 c          299 g          211 t
ORIGIN      
        1 aagatcgagg tacccggtat tccccagggc tgaatgcccg gccaacacgc tgccctggtc
       61 cggtgccgct aacagtatac ttcccatcga accgttatcg tgtgtacggg tcgcctggaa
      121 ttgtaagggt tgggattggg cggataagtg aaagagcagt gatagcaagg tcaggaagaa
      181 aagacgtctc atgtgctggt agttcatagt tcttcctttt cacggatcag tcgcttcatg
      241 cgaatgcgaa cttccgtaat ccaggccatg agcaatcccc atcctagtac ggccgggtag
      301 aagatggtcc gcatgcgtga atccagatca taggaactga agccgggatt ccctccgttg
      361 ccgggatgca gggaatcggt catgcgcggc agaatcatga tgaagacgat catcagcgaa
      421 aaggcgaaga tgctgtaaac ggctgtgatc ctcgcgcgct tatgctcatc ttccatcgag
      481 ccgcgcagga tgagataagc gaagtaaacc aacatcgtag cggccgcgcc gttcaacttg
      541 gcgtcgttta cccaccaggc tccccaggtg ttgcgggccc agatgctgcc ggtgatcagg
      601 cccagtatgc cgaccaccac accggtccgg acacactcgt cggcgcgaag gtcgtcatca
      661 agattccctt tgcgcaaata acggatgccg aagatgagtg cggccatcat caagcccatc
      721 atcgaaaacc acatggtcac atgatagaac aggttgcgga tcgtttcatg caggatggca
      781 agtcggggaa ccgggatgag cattcctccg acgatcgcgt agagtacgag cagcagggaa
      841 agccatttcc accaggcgag tccggcaatc ttcatgagtt gtggtgttga ttgggttggt
      901 agttcactct ttccaaagat agggaaacag caggtatgcg agggtgacga ccagcaggtt
      961 caaggcggcc agcaggatga tgtagtgcgt gctgaccgac cacgccagtc catccatggc
     1021 gtt
//
LOCUS       DAOTON010000986         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_123353_length_1017_cov_2.961538, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000986 DAOTON010000000
VERSION     DAOTON010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123353_length_1017_cov_2.961538"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..>1017
                     /locus_tag="P5292_14395"
     CDS             179..>1017
                     /locus_tag="P5292_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40367.1"
                     /translation="MMNKFVKTTLILLAVALSTQARQNVGQTSTSNGSGGSGSGRIFA
                     GCTPSKSRADLDINNVRTPIWINGDMWWDLVGNAEYEVPKGSGKNSLFAGAIWIGGKD
                     AAGNLKVAAQTYRQSGSDFWPGPVDTRDATITADVCSQYDKHWKITKAEVKDFKDYYD
                     LNGTAAGYPVSDVIKTWPGNGDPSKGQDQFLAPFVDRDNDGFYNWESGDYPKYDYSST
                     PDCSDRNVLLGDQTIWWVFNDVGNIHTETESQFAVGLEIRGHAFAFSTTNEINNMTFY
                     RYQ"
BASE COUNT          240 a          276 c          267 g          234 t
ORIGIN      
        1 tcacccggcg cgcaagcctt catcgactca aaagtcaacc cgcagtccta tatcgacttg
       61 tatgagattg cccagaacaa cccggggaat tatcagttac cacgtcgtat ccgcctgggt
      121 cttcagttga acttctgatc ataattattc cattcgttaa caatccatcc gcgatagcat
      181 gatgaacaag tttgtcaaaa ccacgctgat tctcctggct gtagctctca gcacgcaggc
      241 ccggcagaat gttggtcaga cgagcacgag caacgggagt gggggatcgg gttccggccg
      301 gatcttcgcc ggctgtaccc cgtccaagtc ccgtgcagac ctcgatatca acaacgttcg
      361 tacccccatc tggatcaacg gcgatatgtg gtgggacctc gtcggtaatg ccgagtacga
      421 agtgccgaaa ggttcaggaa agaactccct gttcgccggt gccatctgga tcggcggtaa
      481 ggacgctgcc ggtaacctga aagttgcggc ccaaacctac cgtcagtcgg gttcggactt
      541 ctggcccgga ccggtggata cccgtgatgc taccattact gcggacgttt gttcgcagta
      601 tgacaagcac tggaagatca ccaaggcgga ggttaaggac ttcaaggact actacgacct
      661 caacggaacc gctgcaggat atccggtttc ggatgtgatc aaaacctggc ctggtaacgg
      721 tgacccttcg aaagggcagg atcagttcct cgctccgttc gtggatcgcg acaacgatgg
      781 tttttacaac tgggagtcgg gcgattatcc gaagtacgac tattcttcga cccctgattg
      841 ttccgatcgt aacgtcctgt tgggcgatca gaccatctgg tgggtattca atgacgttgg
      901 aaacatccac acggaaactg aatctcagtt tgcggttgga ctggaaattc gcggacatgc
      961 gtttgccttc tcgacgacca acgagatcaa caacatgacc ttctatcgtt accagat
//
LOCUS       DAOTON010000987         1015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_123793_length_1015_cov_2.463542, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000987 DAOTON010000000
VERSION     DAOTON010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123793_length_1015_cov_2.463542"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..>1015
                     /locus_tag="P5292_14400"
     CDS             101..>1015
                     /locus_tag="P5292_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40368.1"
                     /translation="MLPEYVERVELPAGYNSCTASIVAVQTEPVQDISLLPADAAIKS
                     EFEVSTLIGFRKKQPVATVRVVPIRKSGSGYERLVRFQLQVTPSVNTQRTALRTYSTN
                     SVLASGSWFRIATTQQGVHKMTYQFLKDLGIDVDNIDPRRLRIYGNGGGQLPFNNSKF
                     RYDDLQENAILVQGENDGVFDNTDFVLFFATAQTRWKYDGTGETFSHQLNGYSDSTYY
                     FITTDLGSGKRIQHLAEASGTPNQTVSTYNDYQFIESELVNLLKSGREWYGESLDNLN
                     SSRSYNFSFPNLVTSEQATFRANMIARAV"
BASE COUNT          251 a          293 c          254 g          217 t
ORIGIN      
        1 gacccgatca cttcaatgga ctgcaccttt ccaaaaacaa accagtgaag attcgttttt
       61 ccgatccctc cactttgaag gatgtcatat gaacgaatcc atgctgccgg aatacgtaga
      121 gcgagtggaa cttccggccg gatacaattc ctgcactgct tccatcgtgg ccgtgcaaac
      181 cgaacccgtt caggacatca gccttctgcc ggccgatgct gcgatcaaat cggaattcga
      241 ggtaagtacg ctgatcggat tccgaaagaa gcagccggta gccaccgtgc gcgtagtacc
      301 gattcgcaag tcgggttccg gttacgagcg cctggtccgc ttccaattgc aagtgacgcc
      361 ctcggtaaat acgcagcgca ccgcgttgcg cacctattcg accaatagcg tactcgcaag
      421 cgggagctgg ttccgcatcg ccaccacgca acagggtgta cacaagatga cctatcagtt
      481 cctaaaagac cttggcatcg acgtggataa catcgacccg cgccggctga gaatctatgg
      541 gaacggcggc ggtcagctac ccttcaacaa ctccaagttc cggtacgacg acttacagga
      601 aaatgccatc ctggtgcaag gtgagaatga cggagttttt gacaataccg atttcgtgct
      661 cttcttcgcc accgctcaga cacgctggaa gtatgatggg accggtgaaa cctttagcca
      721 ccagttgaac ggatattcgg attcgacgta ttactttatc acgacggacc ttggttccgg
      781 caaacggatc cagcacctcg cggaagcatc gggaacccct aaccagaccg tttccaccta
      841 taacgattat cagttcatcg agtcagaatt ggtgaacctg ctcaagtcgg gacgtgaatg
      901 gtacggcgag agcctggata atcttaacag cagccgttcc tacaactttt cattcccgaa
      961 cctcgtcacc tccgagcagg caacgttccg ggccaacatg atcgcccggg cggtg
//
LOCUS       DAOTON010000988         1011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_124757_length_1011_cov_1.695607, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000988 DAOTON010000000
VERSION     DAOTON010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124757_length_1011_cov_1.695607"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..>1011
                     /locus_tag="P5292_14405"
     CDS             51..>1011
                     /locus_tag="P5292_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40369.1"
                     /translation="MNLNYGGTGGGNGNVVQGNVISNITGEGAITGITHSSSTTGGVV
                     SFFGNTITGLSSTGTGGSVTALASSSATSHVYSNVIGDLSSTGSASTVTGISISSATA
                     SNIYKNKVYGLTSNGASGIANGITVSAGTTSNIYNNIVGNLSAPTASLANAINGINIT
                     GGTTVNAYYNTVYFSASSSGANFGTTALFANTTPTVTLRNNLLHNASSANGTGLAVAY
                     RRSGTTLTSYGSASNNNDFVAATIYYDGTTPYSWSGYQALVATRDAASVNWTPVYLSL
                     VGNSANFLHIDPAVATLLESGAVNITTPAITDDYDANIRQGNGG"
BASE COUNT          237 a          282 c          242 g          250 t
ORIGIN      
        1 agaataacac gttctcgaac ataaccggag gtaccagtgc catcaccatc atgaacctta
       61 attatggtgg aacgggcggt ggtaatggaa acgttgttca aggaaacgtc atttcgaaca
      121 ttaccggtga gggtgcgata acgggtatta cccattccag cagcaccact ggcggtgtag
      181 tatccttttt cggaaatact attaccggcc ttagttctac cggaacgggt ggttcggtga
      241 cggcacttgc ttccagcagc gctaccagcc acgtttattc caacgtgatc ggcgaccttt
      301 cttcgaccgg ctctgccagc acggtaacag gtatcagcat ttcttcagcc acggcttcga
      361 atatttataa gaacaaagtc tacgggttga cttcgaatgg tgccagtggc atcgctaacg
      421 gtatcacggt ctctgccggt actacgtcca atatctataa taacatcgta ggtaacctgt
      481 cggctcccac ggccagcctt gctaatgcaa ttaacggtat caacattacc ggtggtacga
      541 cagtcaacgc ctactacaat acagtgtatt ttagtgctag cagttcgggt gccaacttcg
      601 gtactactgc tttattcgcg aatacgaccc ctacggtaac acttcgcaac aacttgctgc
      661 ataacgcttc aagtgcgaat ggtacaggct tggctgttgc ctatcgccgg tccggcacca
      721 cgttgacctc ttatggttca gcctccaaca ataacgactt tgtcgcagct accatttact
      781 atgacggtac cactccctat agctggagcg gctaccaggc cctggtggct acccgcgacg
      841 ctgcttctgt caactggact ccggtgtacc tgagcctggt tggtaacagc gccaacttcc
      901 tccacatcga tccggctgtg gccaccctgc tcgaatcggg tgcggtgaac atcaccactc
      961 ccgcgatcac ggacgactat gatgcaaaca tccgccaggg taatggtggt t
//
LOCUS       DAOTON010000989         1004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_126193_length_1004_cov_1.567966, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000989 DAOTON010000000
VERSION     DAOTON010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126193_length_1004_cov_1.567966"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1004)
                     /locus_tag="P5292_14410"
     CDS             complement(<1..>1004)
                     /locus_tag="P5292_14410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRS40370.1"
                     /translation="AMAGESLTIEKQLVLTGKGIDVEIIATPVRNEHRLVVSVSYFGR
                     DISARKQAEAELVRAREQAEQATQAKSSFLATMSHEIRTPLNGVIGMGKLLNQTALSP
                     KQQDYVDSILLSGEALLSVINDILDYSKIESAKMELEYKPFALKRCIEETFDLMASKA
                     IEKKLSLRYSVAKGTPSYIYGDLTRLRQVLMKLVSNAIKFTQQGGITIHVSKMREEGK
                     HVELLFEVQDTGLGIPADRIARLFQSFSQADASTARTYGGTGLGLAICKNLVELMHGS
                     IWVDSVPGQGSNFQFTIRTEAGTATEAVKSGRNGSSRLANAHVLLVSDDRTEADLFAN
                     "
BASE COUNT          227 a          291 c          252 g          234 t
ORIGIN      
        1 tagttggcaa agagatccgc ttccgtgcga tcgtcggata cgagcaacac atgcgcgtta
       61 gccaacctgc tggagccgtt tcgtccgctt ttaaccgctt ccgtcgcggt acccgcttcc
      121 gttcgaatcg tgaactgaaa gttcgaaccc tgccccggca cactatcgac ccagatggat
      181 ccgtgcatca actcgacaag atttttacag atagcaagac caagaccggt accgccgtaa
      241 gtccgcgccg tagaagcatc ggcctgtgaa aacgactgga acaaacgggc gatccgatcg
      301 gccggaatgc caagaccggt atcctgaact tcgaataaca actcaacatg cttgccctct
      361 tcccgcatct tcgagacatg aatggtgata ccaccctgtt gggtgaattt gatggcgttg
      421 gataccagct tcatcaaaac ctgacgcaag cgggtcagat ccccatagat ataggaaggt
      481 gtacctttcg ctactgaata ccgcagcgac aacttctttt caattgcctt ggaggccatg
      541 aggtcaaacg tttcctcgat gcaacgcttg agcgcgaacg gcttgtactc cagctccatc
      601 ttcgccgatt cgatcttgga ataatcgagg atatcgttga tcacggaaag cagcgcttca
      661 ccgctcagca ggatggagtc aacgtagtct tgctgtttgg gtgacagggc ggtttgattg
      721 agcaatttac ccatgccgat cacgccattg agcggcgtgc ggatctcgtg gctcatcgtt
      781 gccaggaaag acgacttcgc ctgggtggct tgttcggctt gttcacgggc acgaaccagt
      841 tccgcttccg cctgcttccg cgcagagata tcccgtccga aataggatac actgaccact
      901 aaccggtgct cattcctaac cggcgtcgcg atgatctcca catcgatccc cttgcctgtc
      961 agcaccagtt gcttctcgat ggtgagcgat tcacccgcca ttgc
//
LOCUS       DAOTON010000990         1003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_126334_length_1003_cov_2.300633, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000990 DAOTON010000000
VERSION     DAOTON010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126334_length_1003_cov_2.300633"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="P5292_14415"
     CDS             <1..798
                     /locus_tag="P5292_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRS40371.1"
                     /translation="GHGAVLRNEAGEEFMKRYDERGSLAPRDIVARAIDNEMKITGED
                     FVFLDCRHLDLEDFRSHFPNILSKCESLGIDIRKAMIPVVPAAHYICGGILVDEHGRS
                     SIQNLYAAGECACTGLHGGNRLASNSLLEALVYAHRSWLHAKEVIDGIRFEERVPDWN
                     AEGTTQPNELVLITHNRRELQEVMSSYVGIVRSNVRLKRALDRLRILYGETEALYERT
                     VLSPMLCELRNMITIGYLIVKAAMARPESVGLHYTLDYPPANQETSA"
     gene            795..>1003
                     /locus_tag="P5292_14420"
     CDS             795..>1003
                     /locus_tag="P5292_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40372.1"
                     /translation="MNRSFADQVFSIRNEQEFESVAMEVFRYQASENPVYREYLRQLQ
                     RDPADLRELRQVPFLPIDFFRTHRVV"
BASE COUNT          222 a          273 c          279 g          229 t
ORIGIN      
        1 gggcacggcg cggtattgcg caacgaggct ggagaggaat tcatgaaacg gtacgatgaa
       61 cgtggctcac ttgctccccg cgatattgtg gcgcgggcca tcgacaacga gatgaagatc
      121 accggggagg atttcgtttt tctggattgc cgtcatctgg acctggagga ttttcgttct
      181 catttcccca atatcctctc gaagtgcgaa tcgctcggca tcgacattcg taaagcgatg
      241 ataccggtgg tgccggccgc ccactatatc tgcggtggga ttttggtcga cgagcatggc
      301 cgttccagta ttcaaaacct ctatgcggcc ggtgaatgtg cctgtaccgg actacacggc
      361 gggaaccgat tggcatccaa ctccctgctc gaagccttgg tctatgcgca tcgatcctgg
      421 ctgcatgcga aagaggtgat cgacggtatc cgcttcgaag agcgcgtacc cgattggaac
      481 gcggaaggta ccacccagcc gaacgagttg gtattgatca cccacaaccg ccgtgaattg
      541 caggaagtga tgagcagtta tgtgggcatt gtccggtcaa acgttcgcct taaacgagcc
      601 ttggaccgat tacgcattct ttatggggaa acagaagccc tctacgaacg cacggtgctg
      661 agtccgatgt tgtgcgagct tcgcaacatg ataacgatcg gttatctgat cgtcaaagcg
      721 gccatggcta gaccggaaag cgtagggctt cattatacgc tcgattaccc tccggcgaat
      781 caggagacat cagcatgaac cgctcgttcg ctgatcaggt attttccatc cgcaacgaac
      841 aggagtttga atcggtcgcg atggaggtat tccggtatca ggcatcggag aaccctgttt
      901 atcgggaata cctgcgacaa ctccagcgcg atccggccga ccttcgcgaa ctgcgccagg
      961 tacccttcct tcccatcgac ttcttccgca cccatcgtgt cgt
//
LOCUS       DAOTON010000991         1002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_2868
            NODE_126534_length_1002_cov_2.642027, whole genome shotgun
            sequence.
ACCESSION   DAOTON010000991 DAOTON010000000
VERSION     DAOTON010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867892
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 17:21:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,886
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 17 of 21
            Pseudo Genes (internal stop)      :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126534_length_1002_cov_2.642027"
                     /isolate="AMR_MDS_2868"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="P5292_14425"
     CDS             complement(<1..465)
                     /locus_tag="P5292_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40373.1"
                     /translation="MDLEQVFPPNFLDKLKQLIKDDKDEFKHEYYRGCVRHCEEMSWH
                     VYGKKPERLLKRVRPREDPAITKYRLDSYEPITQSVCKKALSITHKIFDSGLWAVHFK
                     DDKNSQELKKYTLEKYPRFNSVVNYLANYILKKMIADPNAIILVQPYKYMIKG"
     gene            complement(495..>1002)
                     /locus_tag="P5292_14430"
     CDS             complement(495..>1002)
                     /locus_tag="P5292_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRS40374.1"
                     /translation="AYTQINCGIDQAGIVAVGFIDEHAYSTTPTKVNLESVGFYTNLL
                     NSSPPTLFVATKTRGEYPGGTPTEEDGFGKESTQITGAKHEATIEFEGVEENRNFVEG
                     INSKKWKLVFVLNGGKGVYVDTPATAYGKLVVPRGITTGAFWQMKISWQDFANPQTFD
                     APTSVFAE"
BASE COUNT          238 a          237 c          232 g          295 t
ORIGIN      
        1 tcccttaatc atgtacttgt acggctgaac taaaataatc gcgttagggt ccgcgatcat
       61 ctttttgagg atgtagttag ccaggtagtt taccacgctg ttaaatcgcg ggtacttctc
      121 cagggtgtac ttcttcagtt cctgtgaatt cttgtcgtcc ttgaaatgga ccgcccaaag
      181 ccctgaatcg aaaatcttgt gggtaatcga aagggctttt ttgcagactg attgtgtgat
      241 tggttcgtat gaatccagcc ggtatttggt aattgccgga tcttcacgcg gacggactct
      301 tttcagtagc cgttccggtt ttttcccata gacgtgccat gacatttcct cgcaatgcct
      361 aacgcatcca cgatagtatt cgtgtttgaa ttcgtctttg tcgtccttga ttaactgctt
      421 cagcttatca aggaaattgg gcgggaaaac ctgctctaaa tccatagtag aaaataaggg
      481 actaattcag ccccttattc tgcaaatacc gatgtaggag cgtcgaacgt ctgcgggtta
      541 gcaaagtcct gccagctaat tttcatctgc cagaacgcgc cagtggtaat accgcgaggc
      601 acaaccaact taccgtaagc agtagccggg gtgtcaacat aaacgccttt accgccattg
      661 agaacgaaaa caagtttcca cttcttggag ttgattccct ccacaaaatt tctgttttct
      721 tctacgccct caaattcgat agtggcctca tgctttgcgc cggtgatctg cgtggactct
      781 ttgccgaatc catcttcttc ggtaggtgta cctcctggat attcgccgcg tgttttagtt
      841 gcaacaaaca gggtcggagg ggacgagttg agaagattgg tatagaaacc aaccgattcc
      901 aggttaacct tcgtcggggt ggttgaatac gcatgttcgt caatgaatcc taccgctaca
      961 attccagcct gatcgatacc gcagtttatt tgggtatatg ca
//
